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Sample records for dynamics control cell

  1. Direct Adaptive Aircraft Control Using Dynamic Cell Structure Neural Networks

    Science.gov (United States)

    Jorgensen, Charles C.

    1997-01-01

    A Dynamic Cell Structure (DCS) Neural Network was developed which learns topology representing networks (TRNS) of F-15 aircraft aerodynamic stability and control derivatives. The network is integrated into a direct adaptive tracking controller. The combination produces a robust adaptive architecture capable of handling multiple accident and off- nominal flight scenarios. This paper describes the DCS network and modifications to the parameter estimation procedure. The work represents one step towards an integrated real-time reconfiguration control architecture for rapid prototyping of new aircraft designs. Performance was evaluated using three off-line benchmarks and on-line nonlinear Virtual Reality simulation. Flight control was evaluated under scenarios including differential stabilator lock, soft sensor failure, control and stability derivative variations, and air turbulence.

  2. Intermediate filaments: a dynamic network that controls cell mechanics.

    Science.gov (United States)

    Gruenbaum, Yosef; Aebi, Ueli

    2014-01-01

    In humans the superfamily of intermediate filament (IF) proteins is encoded by more than 70 different genes, which are expressed in a cell- and tissue-specific manner. IFs assemble into approximately 10 nm-wide filaments that account for the principal structural elements at the nuclear periphery, nucleoplasm, and cytoplasm. They are also required for organizing the microtubule and microfilament networks. In this review, we focus on the dynamics of IFs and how modifications regulate it. We also discuss the role of nuclear IF organization in determining nuclear mechanics as well as that of cytoplasmic IFs organization in maintaining cell stiffness, formation of lamellipodia, regulation of cell migration, and permitting cell adhesion.

  3. Topological defects control collective dynamics in neural progenitor cell cultures

    Science.gov (United States)

    Kawaguchi, Kyogo; Kageyama, Ryoichiro; Sano, Masaki

    2017-04-01

    Cultured stem cells have become a standard platform not only for regenerative medicine and developmental biology but also for biophysical studies. Yet, the characterization of cultured stem cells at the level of morphology and of the macroscopic patterns resulting from cell-to-cell interactions remains largely qualitative. Here we report on the collective dynamics of cultured murine neural progenitor cells (NPCs), which are multipotent stem cells that give rise to cells in the central nervous system. At low densities, NPCs moved randomly in an amoeba-like fashion. However, NPCs at high density elongated and aligned their shapes with one another, gliding at relatively high velocities. Although the direction of motion of individual cells reversed stochastically along the axes of alignment, the cells were capable of forming an aligned pattern up to length scales similar to that of the migratory stream observed in the adult brain. The two-dimensional order of alignment within the culture showed a liquid-crystalline pattern containing interspersed topological defects with winding numbers of +1/2 and -1/2 (half-integer due to the nematic feature that arises from the head-tail symmetry of cell-to-cell interaction). We identified rapid cell accumulation at +1/2 defects and the formation of three-dimensional mounds. Imaging at the single-cell level around the defects allowed us to quantify the velocity field and the evolving cell density; cells not only concentrate at +1/2 defects, but also escape from -1/2 defects. We propose a generic mechanism for the instability in cell density around the defects that arises from the interplay between the anisotropic friction and the active force field.

  4. Hoxb1b controls oriented cell division, cell shape and microtubule dynamics in neural tube morphogenesis.

    Science.gov (United States)

    Zigman, Mihaela; Laumann-Lipp, Nico; Titus, Tom; Postlethwait, John; Moens, Cecilia B

    2014-02-01

    Hox genes are classically ascribed to function in patterning the anterior-posterior axis of bilaterian animals; however, their role in directing molecular mechanisms underlying morphogenesis at the cellular level remains largely unstudied. We unveil a non-classical role for the zebrafish hoxb1b gene, which shares ancestral functions with mammalian Hoxa1, in controlling progenitor cell shape and oriented cell division during zebrafish anterior hindbrain neural tube morphogenesis. This is likely distinct from its role in cell fate acquisition and segment boundary formation. We show that, without affecting major components of apico-basal or planar cell polarity, Hoxb1b regulates mitotic spindle rotation during the oriented neural keel symmetric mitoses that are required for normal neural tube lumen formation in the zebrafish. This function correlates with a non-cell-autonomous requirement for Hoxb1b in regulating microtubule plus-end dynamics in progenitor cells in interphase. We propose that Hox genes can influence global tissue morphogenesis by control of microtubule dynamics in individual cells in vivo.

  5. Multiple control and dynamic response of the Xenopus melanotrope cell.

    NARCIS (Netherlands)

    Kolk, S.M.; Kramer, B.M.; Cornelisse, L.N.; Scheenen, W.J.J.M.; Jenks, B.G.; Roubos, E.W.

    2002-01-01

    Some amphibian brain-melanotrope cell systems are used to study how neuronal and (neuro)endocrine mechanisms convert environmental signals into physiological responses. Pituitary melanotropes release alpha-melanophore-stimulating hormone (alpha-MSH), which controls skin color in response to backgrou

  6. Dynamic Thermal Model and Temperature Control of Proton Exchange Membrane Fuel Cell Stack

    Institute of Scientific and Technical Information of China (English)

    邵庆龙; 卫东; 曹广益; 朱新坚

    2005-01-01

    A dynamic thermal transfer model of a proton exchange membrane fuel cell (PEMFC) stack is developed based on energy conservation in order to reach better temperature control of PEMFC stack. Considering its uncertain parameters and disturbance, we propose a robust adaptive controller based on backstepping algorithm of Lyaponov function. Numerical simulations indicate the validity of the proposed controller.

  7. Improving dynamic performance of proton-exchange membrane fuel cell system using time delay control

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Young-Bae [Mechanical Engineering Department, Chonnam National University, Gwangju (Korea)

    2010-10-01

    Transient behaviour is a key parameter for the vehicular application of proton-exchange membrane (PEM) fuel cell. The goal of this presentation is to construct better control technology to increase the dynamic performance of a PEM fuel cell. The PEM fuel cell model comprises a compressor, an injection pump, a humidifier, a cooler, inlet and outlet manifolds, and a membrane-electrode assembly. The model includes the dynamic states of current, voltage, relative humidity, stoichiometry of air and hydrogen, cathode and anode pressures, cathode and anode mass flow rates, and power. Anode recirculation is also included with the injection pump, as well as anode purging, for preventing anode flooding. A steady-state, isothermal analytical fuel cell model is constructed to analyze the mass transfer and water transportation in the membrane. In order to prevent the starvation of air and flooding in a PEM fuel cell, time delay control is suggested to regulate the optimum stoichiometry of oxygen and hydrogen, even when there are dynamical fluctuations of the required PEM fuel cell power. To prove the dynamical performance improvement of the present method, feed-forward control and Linear Quadratic Gaussian (LQG) control with a state estimator are compared. Matlab/Simulink simulation is performed to validate the proposed methodology to increase the dynamic performance of a PEM fuel cell system. (author)

  8. Improving dynamic performance of proton-exchange membrane fuel cell system using time delay control

    Science.gov (United States)

    Kim, Young-Bae

    Transient behaviour is a key parameter for the vehicular application of proton-exchange membrane (PEM) fuel cell. The goal of this presentation is to construct better control technology to increase the dynamic performance of a PEM fuel cell. The PEM fuel cell model comprises a compressor, an injection pump, a humidifier, a cooler, inlet and outlet manifolds, and a membrane-electrode assembly. The model includes the dynamic states of current, voltage, relative humidity, stoichiometry of air and hydrogen, cathode and anode pressures, cathode and anode mass flow rates, and power. Anode recirculation is also included with the injection pump, as well as anode purging, for preventing anode flooding. A steady-state, isothermal analytical fuel cell model is constructed to analyze the mass transfer and water transportation in the membrane. In order to prevent the starvation of air and flooding in a PEM fuel cell, time delay control is suggested to regulate the optimum stoichiometry of oxygen and hydrogen, even when there are dynamical fluctuations of the required PEM fuel cell power. To prove the dynamical performance improvement of the present method, feed-forward control and Linear Quadratic Gaussian (LQG) control with a state estimator are compared. Matlab/Simulink simulation is performed to validate the proposed methodology to increase the dynamic performance of a PEM fuel cell system.

  9. Chronophin coordinates cell leading edge dynamics by controlling active cofilin levels

    Science.gov (United States)

    Delorme-Walker, Violaine; Seo, Ji-Yeon; Gohla, Antje; Fowler, Bruce; Bohl, Ben; DerMardirossian, Céline

    2015-01-01

    Cofilin, a critical player of actin dynamics, is spatially and temporally regulated to control the direction and force of membrane extension required for cell locomotion. In carcinoma cells, although the signaling pathways regulating cofilin activity to control cell direction have been established, the molecular machinery required to generate the force of the protrusion remains unclear. We show that the cofilin phosphatase chronophin (CIN) spatiotemporally regulates cofilin activity at the cell edge to generate persistent membrane extension. We show that CIN translocates to the leading edge in a PI3-kinase–, Rac1-, and cofilin-dependent manner after EGF stimulation to activate cofilin, promotes actin free barbed end formation, accelerates actin turnover, and enhances membrane protrusion. In addition, we establish that CIN is crucial for the balance of protrusion/retraction events during cell migration. Thus, CIN coordinates the leading edge dynamics by controlling active cofilin levels to promote MTLn3 cell protrusion. PMID:26324884

  10. Neuropeptides control the dynamic behavior of airway mucosal dendritic cells.

    Science.gov (United States)

    Voedisch, Sabrina; Rochlitzer, Sabine; Veres, Tibor Z; Spies, Emma; Braun, Armin

    2012-01-01

    The airway mucosal epithelium is permanently exposed to airborne particles. A network of immune cells patrols at this interface to the environment. The interplay of immune cells is orchestrated by different mediators. In the current study we investigated the impact of neuronal signals on key functions of dendritic cells (DC). Using two-photon microscopic time-lapse analysis of living lung sections from CD11c-EYFP transgenic mice we studied the influence of neuropeptides on airway DC motility. Additionally, using a confocal microscopic approach, the phagocytotic capacity of CD11c(+) cells after neuropeptide stimulation was determined. Electrical field stimulation (EFS) leads to an unspecific release of neuropeptides from nerves. After EFS and treatment with the neuropeptides vasoactive intestinal peptide (VIP) or calcitonin gene-related peptide (CGRP), airway DC in living lung slices showed an altered motility. Furthermore, the EFS-mediated effect could partially be blocked by pre-treatment with the receptor antagonist CGRP(8-37). Additionally, the phagocytotic capacity of bone marrow-derived and whole lung CD11c(+) cells could be inhibited by neuropeptides CGRP, VIP, and Substance P. We then cross-linked these data with the in vivo situation by analyzing DC motility in two different OVA asthma models. Both in the acute and prolonged OVA asthma model altered neuropeptide amounts and DC motility in the airways could be measured. In summary, our data suggest that neuropeptides modulate key features motility and phagocytosis of mouse airway DC. Therefore altered neuropeptide levels in airways during allergic inflammation have impact on regulation of airway immune mechanisms and therefore might contribute to the pathophysiology of asthma.

  11. Molecular Network Dynamics of Cell Cycle Control: Periodicity of Start and Finish.

    Science.gov (United States)

    Palmisano, Alida; Zámborszky, Judit; Oguz, Cihan; Csikász-Nagy, Attila

    2017-01-01

    The cell division cycle is controlled by a complex regulatory network which ensures that the phases of the cell cycle are executed in the right order. This regulatory network receives signals from the environment, monitors the state of the DNA, and decides timings of cell cycle events. The underlying transcriptional and post-translational regulatory interactions lead to complex dynamical responses, such as the oscillations in the levels of cell cycle proteins driven by intertwined biochemical reactions. A cell moves between different phases of its cycle similar to a dynamical system switching between its steady states. The complex molecular network driving these phases has been investigated in previous computational systems biology studies. Here, we review the critical physiological and molecular transitions that occur in the cell cycle and discuss the role of mathematical modeling in elucidating these transitions and understand cell cycle synchronization.

  12. A genetic strategy for the dynamic and graded control of cell mechanics, motility, and matrix remodeling.

    Science.gov (United States)

    MacKay, Joanna L; Keung, Albert J; Kumar, Sanjay

    2012-02-08

    Cellular mechanical properties have emerged as central regulators of many critical cell behaviors, including proliferation, motility, and differentiation. Although investigators have developed numerous techniques to influence these properties indirectly by engineering the extracellular matrix (ECM), relatively few tools are available to directly engineer the cells themselves. Here we present a genetic strategy for obtaining graded, dynamic control over cellular mechanical properties by regulating the expression of mutant mechanotransductive proteins from a single copy of a gene placed under a repressible promoter. With the use of constitutively active mutants of RhoA GTPase and myosin light chain kinase, we show that varying the expression level of either protein produces graded changes in stress fiber assembly, traction force generation, cellular stiffness, and migration speed. Using this approach, we demonstrate that soft ECMs render cells maximally sensitive to changes in RhoA activity, and that by modulating the ability of cells to engage and contract soft ECMs, we can dynamically control cell spreading, migration, and matrix remodeling. Thus, in addition to providing quantitative relationships between mechanotransductive signaling, cellular mechanical properties, and dynamic cell behaviors, this strategy enables us to control the physical interactions between cells and the ECM and thereby dictate how cells respond to matrix properties.

  13. A polarised population of dynamic microtubules mediates homeostatic length control in animal cells.

    Directory of Open Access Journals (Sweden)

    Remigio Picone

    Full Text Available Because physical form and function are intimately linked, mechanisms that maintain cell shape and size within strict limits are likely to be important for a wide variety of biological processes. However, while intrinsic controls have been found to contribute to the relatively well-defined shape of bacteria and yeast cells, the extent to which individual cells from a multicellular animal control their plastic form remains unclear. Here, using micropatterned lines to limit cell extension to one dimension, we show that cells spread to a characteristic steady-state length that is independent of cell size, pattern width, and cortical actin. Instead, homeostatic length control on lines depends on a population of dynamic microtubules that lead during cell extension, and that are aligned along the long cell axis as the result of interactions of microtubule plus ends with the lateral cell cortex. Similarly, during the development of the zebrafish neural tube, elongated neuroepithelial cells maintain a relatively well-defined length that is independent of cell size but dependent upon oriented microtubules. A simple, quantitative model of cellular extension driven by microtubules recapitulates cell elongation on lines, the steady-state distribution of microtubules, and cell length homeostasis, and predicts the effects of microtubule inhibitors on cell length. Together this experimental and theoretical analysis suggests that microtubule dynamics impose unexpected limits on cell geometry that enable cells to regulate their length. Since cells are the building blocks and architects of tissue morphogenesis, such intrinsically defined limits may be important for development and homeostasis in multicellular organisms.

  14. Slug controls stem/progenitor cell growth dynamics during mammary gland morphogenesis.

    Directory of Open Access Journals (Sweden)

    Mayssa Nassour

    Full Text Available BACKGROUND: Morphogenesis results from the coordination of distinct cell signaling pathways controlling migration, differentiation, apoptosis, and proliferation, along stem/progenitor cell dynamics. To decipher this puzzle, we focused on epithelial-mesenchymal transition (EMT "master genes". EMT has emerged as a unifying concept, involving cell-cell adhesion, migration and apoptotic pathways. EMT also appears to mingle with stemness. However, very little is known on the physiological role and relevance of EMT master-genes. We addressed this question during mammary morphogenesis. Recently, a link between Slug/Snai2 and stemness has been described in mammary epithelial cells, but EMT master genes actual localization, role and targets during mammary gland morphogenesis are not known and we focused on this basic question. METHODOLOGY/PRINCIPAL FINDINGS: Using a Slug-lacZ transgenic model and immunolocalization, we located Slug in a distinct subpopulation covering about 10-20% basal cap and duct cells, mostly cycling cells, coexpressed with basal markers P-cadherin, CK5 and CD49f. During puberty, Slug-deficient mammary epithelium exhibited a delayed development after transplantation, contained less cycling cells, and overexpressed CK8/18, ER, GATA3 and BMI1 genes, linked to luminal lineage. Other EMT master genes were overexpressed, suggesting compensation mechanisms. Gain/loss-of-function in vitro experiments confirmed Slug control of mammary epithelial cell luminal differentiation and proliferation. In addition, they showed that Slug enhances specifically clonal mammosphere emergence and growth, cell motility, and represses apoptosis. Strikingly, Slug-deprived mammary epithelial cells lost their potential to generate secondary clonal mammospheres. CONCLUSIONS/SIGNIFICANCE: We conclude that Slug pathway controls the growth dynamics of a subpopulation of cycling progenitor basal cells during mammary morphogenesis. Overall, our data better define a

  15. Slug Controls Stem/Progenitor Cell Growth Dynamics during Mammary Gland Morphogenesis

    Science.gov (United States)

    Selmi, Abdelkader; Côme, Christophe; Faraldo, Maria-Luisa M.; Deugnier, Marie-Ange; Savagner, Pierre

    2012-01-01

    Background Morphogenesis results from the coordination of distinct cell signaling pathways controlling migration, differentiation, apoptosis, and proliferation, along stem/progenitor cell dynamics. To decipher this puzzle, we focused on epithelial-mesenchymal transition (EMT) “master genes”. EMT has emerged as a unifying concept, involving cell-cell adhesion, migration and apoptotic pathways. EMT also appears to mingle with stemness. However, very little is known on the physiological role and relevance of EMT master-genes. We addressed this question during mammary morphogenesis. Recently, a link between Slug/Snai2 and stemness has been described in mammary epithelial cells, but EMT master genes actual localization, role and targets during mammary gland morphogenesis are not known and we focused on this basic question. Methodology/Principal Findings Using a Slug–lacZ transgenic model and immunolocalization, we located Slug in a distinct subpopulation covering about 10–20% basal cap and duct cells, mostly cycling cells, coexpressed with basal markers P-cadherin, CK5 and CD49f. During puberty, Slug-deficient mammary epithelium exhibited a delayed development after transplantation, contained less cycling cells, and overexpressed CK8/18, ER, GATA3 and BMI1 genes, linked to luminal lineage. Other EMT master genes were overexpressed, suggesting compensation mechanisms. Gain/loss-of-function in vitro experiments confirmed Slug control of mammary epithelial cell luminal differentiation and proliferation. In addition, they showed that Slug enhances specifically clonal mammosphere emergence and growth, cell motility, and represses apoptosis. Strikingly, Slug-deprived mammary epithelial cells lost their potential to generate secondary clonal mammospheres. Conclusions/Significance We conclude that Slug pathway controls the growth dynamics of a subpopulation of cycling progenitor basal cells during mammary morphogenesis. Overall, our data better define a key mechanism

  16. Transcriptional regulation is a major controller of cell cycle transition dynamics

    DEFF Research Database (Denmark)

    Romanel, Alessandro; Jensen, Lars Juhl; Cardelli, Luca

    2012-01-01

    in various organisms showed the importance of positive feedbacks in other transitions as well. Here we investigate if a universal control system with transcriptional regulation(s) and post-translational positive feedback(s) can be proposed for the regulation of all cell cycle transitions. Through...... computational modeling, we analyze the transition dynamics in all possible combinations of transcriptional and post-translational regulations. We find that some combinations lead to 'sloppy' transitions, while others give very precise control. The periodic transcriptional regulation through the activator...... or the inhibitor leads to radically different dynamics. Experimental evidence shows that in cell cycle transitions of organisms investigated for cell cycle dependent periodic transcription, only the inhibitor OR the activator is under cyclic control and never both of them. Based on these observations, we propose...

  17. Dynamic Control of Electric Output Characteristics of Proton Exchange Membrane Fuel Cell System

    Institute of Scientific and Technical Information of China (English)

    刘星则; 朱新坚

    2005-01-01

    This paper discusses dynamic characteristics of proton exchange membrane fuel cell (PEMFC) under rapid fluctuation of power demand. Wavelet neural network is adopted in the identification of the characteristic curve to predict the voltage. The system control scheme of the voltage and power is introduced. The corresponding schemes for voltage and power control are studied. MATLAB is used to simulate the control system. The results reveal that the adopted control schemes can produce expected effects. Corresponding anti-disturbance and robustness simulation are also carried out. The simulation results show that the implemented control schemes have better robustness and adaptability.

  18. Hybrid Dynamic Modeling and Control of Molten Carbonate Fuel Cell Stack Shutdown

    Institute of Scientific and Technical Information of China (English)

    LI Yong; CAO Guang-yi; ZHU Xin-jian

    2007-01-01

    A hybrid automaton modeling approach that incorporates state space partitioning, phase dynamic modeling and control law synthesis by control strategy is utilized to develop a hybrid automaton model of molten carbonate fuel cell (MCFC) stack shutdown. The shutdown operation is divided into several phases and their boundaries are decided according to a control strategy, which is a set of specifications about the dynamics of MCFC stack during shutdown. According to the control strategy, the specification of increasing stack temperature is satisfied in a phase that can be modeled accurately. The model for phase that has complex dynamic is approximated. The duration of this kind of phase is decreased to minimize the error caused by model approximation.

  19. Cdc42-dependent actin dynamics controls maturation and secretory activity of dendritic cells

    DEFF Research Database (Denmark)

    Schulz, Anna M; Stutte, Susanne; Hogl, Sebastian

    2015-01-01

    Cell division cycle 42 (Cdc42) is a member of the Rho guanosine triphosphatase family and has pivotal functions in actin organization, cell migration, and proliferation. To further study the molecular mechanisms of dendritic cell (DC) regulation by Cdc42, we used Cdc42-deficient DCs. Cdc42...... disruption, our results propose that Cdc42 control of actin dynamics keeps DCs in an immature state, and cessation of Cdc42 activity during DC maturation facilitates secretion as well as rapid up-regulation of intracellular molecules to the cell surface....

  20. Fuel cell-gas turbine hybrid system design part II: Dynamics and control

    Science.gov (United States)

    McLarty, Dustin; Brouwer, Jack; Samuelsen, Scott

    2014-05-01

    Fuel cell gas turbine hybrid systems have achieved ultra-high efficiency and ultra-low emissions at small scales, but have yet to demonstrate effective dynamic responsiveness or base-load cost savings. Fuel cell systems and hybrid prototypes have not utilized controls to address thermal cycling during load following operation, and have thus been relegated to the less valuable base-load and peak shaving power market. Additionally, pressurized hybrid topping cycles have exhibited increased stall/surge characteristics particularly during off-design operation. This paper evaluates additional control actuators with simple control methods capable of mitigating spatial temperature variation and stall/surge risk during load following operation of hybrid fuel cell systems. The novel use of detailed, spatially resolved, physical fuel cell and turbine models in an integrated system simulation enables the development and evaluation of these additional control methods. It is shown that the hybrid system can achieve greater dynamic response over a larger operating envelope than either individual sub-system; the fuel cell or gas turbine. Results indicate that a combined feed-forward, P-I and cascade control strategy is capable of handling moderate perturbations and achieving a 2:1 (MCFC) or 4:1 (SOFC) turndown ratio while retaining >65% fuel-to-electricity efficiency, while maintaining an acceptable stack temperature profile and stall/surge margin.

  1. Control-oriented dynamic fuzzy model and predictive control for proton exchange membrane fuel cell stack

    Institute of Scientific and Technical Information of China (English)

    LI Xi; DENG Zhong-hua; CAO Guang-yi; ZHU Xin-jian; WEI Dong

    2006-01-01

    Proton exchange membrane fuel cell (PEMFC) stack temperature and cathode stoichiometric oxygen are very important control parameters. The performance and lifespan of PEMFC stack are greatly dependent on the parameters. So, in order to improve the performance index, tight control of two parameters within a given range and reducing their fluctuation are indispensable.However, control-oriented models and control strategies are very weak junctures in the PEMFC development. A predictive control algorithm was presented based on their model established by input-output data and operating experiences. It adjusts the operating temperature to 80 ℃. At the same time, the optimized region of stoichiometric oxygen is kept between 1.8-2.2. Furthermore, the control algorithm adjusts the variants quickly to the destination value and makes the fluctuation of the variants the least. According to the test results, compared with traditional fuzzy and PID controllers, the designed controller shows much better performance.

  2. STK16 regulates actin dynamics to control Golgi organization and cell cycle

    Science.gov (United States)

    Liu, Juanjuan; Yang, Xingxing; Li, Binhua; Wang, Junjun; Wang, Wenchao; Liu, Jing; Liu, Qingsong; Zhang, Xin

    2017-01-01

    STK16 is a ubiquitously expressed, myristoylated, and palmitoylated serine/threonine protein kinase with underexplored functions. Recently, it was shown to be involved in cell division but the mechanism remains unclear. Here we found that human STK16 localizes to the Golgi complex throughout the cell cycle and plays important roles in Golgi structure regulation. STK16 knockdown or kinase inhibition disrupts actin polymers and causes fragmented Golgi in cells. In vitro assays show that STK16 directly binds to actin and regulates actin dynamics in a concentration- and kinase activity-dependent way. In addition, STK16 knockdown or kinase inhibition not only delays mitotic entry and prolongs mitosis, but also causes prometaphase and cytokinesis arrest. Therefore, we revealed STK16 as a novel actin binding protein that resides in the Golgi, which regulates actin dynamics to control Golgi structure and participate in cell cycle progression. PMID:28294156

  3. Experimental characterization of active acoustic metamaterial cell with controllable dynamic density

    Science.gov (United States)

    Akl, Wael; Baz, Amr

    2012-10-01

    Controlling wave propagation pattern within acoustic fluid domains has been the motivation for the acoustic metamaterials developments to target applications ranging from acoustic cloaking to passive noise control techniques. Currently, various numerical and analytical approaches exist to predict the fluid domain material properties necessary for specific propagation pattern. Physical attempts to realize such material properties have revealed engineered material constructions that are focused on predefined wave propagation patterns. In the current paper, coupled fluid-structure one-dimensional metamaterial cell, in which piezoelectric active ingredient has been introduced, is manufactured to achieve controllable dynamic density. The density-controllable cell has been manufactured by coupling a water-filled cavity with piezoelectric elements in a cell of 4.5 cm length and 4.1 cm diameter subject to impulse excitation. A finite element model of the cell has been developed and its predictions are validated against the experimental results. The validated model is utilized to predict the changes in the pressure gradient inside the developed cell which is a direct measure of the changes introduced to the dynamic density of the acoustic metamaterial domain. With such predictions, it is demonstrated that densities as high as 3.2 gm/cm3 and as low as 0.72 gm/cm3 can be achieved experimentally for excitation frequencies ranging between 100 Hz and 500 Hz.

  4. Dynamics & Control

    Science.gov (United States)

    2012-03-06

    DISTRIBUTION A: Approved for public release; distribution is unlimited. Quantum Linear Systems Theory : Matt James, Ian Peterson • Why Quantum Control? Is...Classical linear systems theory has a history going back some 50 years, to the birth of modern control theory with Kalman’s foundational work on filtering...and LQG optimal control. Gaussian Distributions play a fundamental role in classical linear systems theory . • The PIs have shown that classical

  5. Dynamic modeling and controllability analysis of an ethanol reformer for fuel cell application

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, Vanesa M.; Serra, Maria; Riera, Jordi [Institut de Robotica i Informatica Industrial (CSIC-UPC), Llorens i Artigas 4-6, 08028 Barcelona (Spain); Lopez, Eduardo [Institut de Tecniques Energetiques, Universitat Politecnica de Catalunya, Diagonal 647, ed. ETSEIB, 08028 Barcelona (Spain); Planta Piloto de Ingenieria Quimica (CONICET-UNS), Camino de la Carrindanga km7, 8000 Bahia Blanca (Argentina); Llorca, Jordi [Institut de Tecniques Energetiques, Universitat Politecnica de Catalunya, Diagonal 647, ed. ETSEIB, 08028 Barcelona (Spain)

    2010-09-15

    This work presents a controllability analysis of a low temperature ethanol reformer based on a cobalt catalyst for fuel cell application. The study is based on a non-linear dynamic model of a reformer which operates in three separate stages: ethanol dehydrogenation to acetaldehyde and hydrogen, acetaldehyde steam reforming, and water-gas-shift reaction. The controllability analysis is focused on the rapid dynamics due to mass balances and is based on a linearization of the complex non-linear model of the reformer. RGA, CN and MRI analysis tools are applied to the linear model suggesting that a good performance can be obtained with decentralized control for frequencies up to 0.1 rad s{sup -1}. (author)

  6. Microtubule-associated protein-4 controls nanovesicle dynamics and T cell activation.

    Science.gov (United States)

    Bustos-Morán, Eugenio; Blas-Rus, Noelia; Martin-Cófreces, Noa Beatriz; Sánchez-Madrid, Francisco

    2017-04-01

    The immune synapse (IS) is a specialized structure formed at the contact area between T lymphocytes and antigen-presenting cells (APCs) that is essential for the adaptive immune response. Proper T cell activation requires its polarization towards the APC, which is highly dependent on the tubulin cytoskeleton. Microtubule-associated protein-4 (MAP4) is a microtubule (MT)-stabilizing protein that controls MTs in physiological processes, such as cell division, migration, vesicular transport or primary cilia formation. In this study, we assessed the role of MAP4 in T cell activation. MAP4 decorates the pericentrosomal area and MTs of the T cell, and it is involved in MT detyrosination and stable assembly in response to T cell activation. In addition, MAP4 prompts the timely translocation of the MT-organizing center (MTOC) towards the IS and the dynamics of signaling nanovesicles that sustains T cell activation. However, MAP4 acts as a negative regulator of other T cell activation-related signals, including diacylglycerol (DAG) production and IL2 secretion. Our data indicate that MAP4 acts as a checkpoint molecule that balances positive and negative hallmarks of T cell activation.

  7. Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures

    Science.gov (United States)

    Cerruti, Benedetta; Puliafito, Alberto; Shewan, Annette M.; Yu, Wei; Combes, Alexander N.; Little, Melissa H.; Chianale, Federica; Primo, Luca; Serini, Guido; Mostov, Keith E.; Celani, Antonio

    2013-01-01

    The growth of a well-formed epithelial structure is governed by mechanical constraints, cellular apico-basal polarity, and spatially controlled cell division. Here we compared the predictions of a mathematical model of epithelial growth with the morphological analysis of 3D epithelial structures. In both in vitro cyst models and in developing epithelial structures in vivo, epithelial growth could take place close to or far from mechanical equilibrium, and was determined by the hierarchy of time-scales of cell division, cell–cell rearrangements, and lumen dynamics. Equilibrium properties could be inferred by the analysis of cell–cell contact topologies, and the nonequilibrium phenotype was altered by inhibiting ROCK activity. The occurrence of an aberrant multilumen phenotype was linked to fast nonequilibrium growth, even when geometric control of cell division was correctly enforced. We predicted and verified experimentally that slowing down cell division partially rescued a multilumen phenotype induced by altered polarity. These results improve our understanding of the development of epithelial organs and, ultimately, of carcinogenesis. PMID:24145168

  8. The planar cell polarity effector protein Wdpcp (Fritz) controls epithelial cell cortex dynamics via septins and actomyosin.

    Science.gov (United States)

    Park, Tae Joo; Kim, Su Kyoung; Wallingford, John B

    2015-01-09

    Planar cell polarity (PCP) signaling controls polarized behaviors in diverse tissues, including the collective cell movements of gastrulation and the planar polarized beating of motile cilia. A major question in PCP signaling concerns the mechanisms linking this signaling cascade with more general cytoskeletal elements to drive polarized behavior. Previously, we reported that the PCP effector protein Wdpcp (formerly known as Fritz) interacts with septins and is critical for collective cell migration and cilia formation. Here, we report that Wdpcp is broadly involved in maintaining cortical tension in epithelial cells. In vivo 3D time-lapse imaging revealed that Wdpcp is necessary for basolateral plasma membrane stability in epithelial tissues, and we further show that Wdpcp controls cortical septin localization to maintain cortical rigidity in mucociliary epithelial cells. Finally, we show that Wdpcp acts via actomyosin to maintain balanced cortical tension in the epithelium. These data suggest that, in addition to its role in controlling plasma membrane dynamics in collective mesenchymal cell movements, Wdpcp is also essential for normal cell cortex stability during epithelial homeostasis.

  9. Cdc42-dependent actin dynamics controls maturation and secretory activity of dendritic cells

    Science.gov (United States)

    Schulz, Anna M.; Stutte, Susanne; Hogl, Sebastian; Luckashenak, Nancy; Dudziak, Diana; Leroy, Céline; Forné, Ignasi; Imhof, Axel; Müller, Stephan A.; Brakebusch, Cord H.; Lichtenthaler, Stefan F.

    2015-01-01

    Cell division cycle 42 (Cdc42) is a member of the Rho guanosine triphosphatase family and has pivotal functions in actin organization, cell migration, and proliferation. To further study the molecular mechanisms of dendritic cell (DC) regulation by Cdc42, we used Cdc42-deficient DCs. Cdc42 deficiency renders DCs phenotypically mature as they up-regulate the co-stimulatory molecule CD86 from intracellular storages to the cell surface. Cdc42 knockout DCs also accumulate high amounts of invariant chain–major histocompatibility complex (MHC) class II complexes at the cell surface, which cannot efficiently present peptide antigens (Ag’s) for priming of Ag-specific CD4 T cells. Proteome analyses showed a significant reduction in lysosomal MHC class II–processing proteins, such as cathepsins, which are lost from DCs by enhanced secretion. As these effects on DCs can be mimicked by chemical actin disruption, our results propose that Cdc42 control of actin dynamics keeps DCs in an immature state, and cessation of Cdc42 activity during DC maturation facilitates secretion as well as rapid up-regulation of intracellular molecules to the cell surface. PMID:26553928

  10. Sox17-Mediated XEN Cell Conversion Identifies Dynamic Networks Controlling Cell-Fate Decisions in Embryo-Derived Stem Cells

    Directory of Open Access Journals (Sweden)

    Angela C.H. McDonald

    2014-10-01

    Full Text Available Little is known about the gene regulatory networks (GRNs distinguishing extraembryonic endoderm (ExEn stem (XEN cells from those that maintain the extensively characterized embryonic stem cell (ESC. An intriguing network candidate is Sox17, an essential transcription factor for XEN derivation and self-renewal. Here, we show that forced Sox17 expression drives ESCs toward ExEn, generating XEN cells that contribute to ExEn when placed back into early mouse embryos. Transient Sox17 expression is sufficient to drive this fate change during which time cells transit through distinct intermediate states prior to the generation of functional XEN-like cells. To orchestrate this conversion process, Sox17 acts in autoregulatory and feedforward network motifs, regulating dynamic GRNs directing cell fate. Sox17-mediated XEN conversion helps to explain the regulation of cell-fate changes and reveals GRNs regulating lineage decisions in the mouse embryo.

  11. Control of vertebrate core planar cell polarity protein localization and dynamics by Prickle 2.

    Science.gov (United States)

    Butler, Mitchell T; Wallingford, John B

    2015-10-01

    Planar cell polarity (PCP) is a ubiquitous property of animal tissues and is essential for morphogenesis and homeostasis. In most cases, this fundamental property is governed by a deeply conserved set of 'core PCP' proteins, which includes the transmembrane proteins Van Gogh-like (Vangl) and Frizzled (Fzd), as well as the cytoplasmic effectors Prickle (Pk) and Dishevelled (Dvl). Asymmetric localization of these proteins is thought to be central to their function, and understanding the dynamics of these proteins is an important challenge in developmental biology. Among the processes that are organized by the core PCP proteins is the directional beating of cilia, such as those in the vertebrate node, airway and brain. Here, we exploit the live imaging capabilities of Xenopus to chart the progressive asymmetric localization of fluorescent reporters of Dvl1, Pk2 and Vangl1 in a planar polarized ciliated epithelium. Using this system, we also characterize the influence of Pk2 on the asymmetric dynamics of Vangl1 at the cell cortex, and we define regions of Pk2 that control its own localization and those impacting Vangl1. Finally, our data reveal a striking uncoupling of Vangl1 and Dvl1 asymmetry. This study advances our understanding of conserved PCP protein functions and also establishes a rapid, tractable platform to facilitate future in vivo studies of vertebrate PCP protein dynamics.

  12. Exploring the Altered Dynamics of Mammalian Central Carbon Metabolic Pathway in Cancer Cells: A Classical Control Theoretic Approach.

    Directory of Open Access Journals (Sweden)

    Debjyoti Paul

    Full Text Available In contrast with normal cells, most of the cancer cells depend on aerobic glycolysis for energy production in the form of adenosine triphosphate (ATP bypassing mitochondrial oxidative phosphorylation. Moreover, compared to normal cells, cancer cells exhibit higher consumption of glucose with higher production of lactate. Again, higher rate of glycolysis provides the necessary glycolytic intermediary precursors for DNA, protein and lipid synthesis to maintain high active proliferation of the tumor cells. In this scenario, classical control theory based approach may be useful to explore the altered dynamics of the cancer cells. Since the dynamics of the cancer cells is different from that of the normal cells, understanding their dynamics may lead to development of novel therapeutic strategies.We have developed a model based on the state space equations of classical control theory along with an order reduction technique to mimic the actual dynamic behavior of mammalian central carbon metabolic (CCM pathway in normal cells. Here, we have modified Michaelis Menten kinetic equation to incorporate feedback mechanism along with perturbations and cross talks associated with a metabolic pathway. Furthermore, we have perturbed the proposed model to reduce the mitochondrial oxidative phosphorylation. Thereafter, we have connected proportional-integral (PI controller(s with the model for tuning it to behave like the CCM pathway of a cancer cell. This methodology allows one to track the altered dynamics mediated by different enzymes.The proposed model successfully mimics all the probable dynamics of the CCM pathway in normal cells. Moreover, experimental results demonstrate that in cancer cells, a coordination among enzymes catalyzing pentose phosphate pathway and intermediate glycolytic enzymes along with switching of pyruvate kinase (M2 isoform plays an important role to maintain their altered dynamics.

  13. Dynamic Thermal Model And Control Of A Pem Fuel Cell System

    DEFF Research Database (Denmark)

    Liso, Vincenzo; Nielsen, Mads Pagh

    2013-01-01

    A lumped parameter dynamic model is developed for predicting the stack performance, temperatures of the exit reactant gases and coolant liquid outlet in a proton-exchange membrane fuel cell (PEMFC) system. The air compressor, humidifier and cooling heat exchanger models are integrated to study...... the fuel cell system. A PID temperature control is implemented to study the effect of stack temperature on settling times of other variables such as stack voltage, air flow rate, oxygen excess ratio and net power of the stack. The model allows an assessment of the effect of operating parameters (stack...... power output, cooling water flow rate, air flow rate, and environmental temperature) and parameter interactions on the system thermal performance. The model represents a useful tool to determine the operating temperatures of the various components of the thermal system, and thus to fully assess...

  14. Bidirectional control of the inner dynamics of focal adhesions promotes cell migration

    OpenAIRE

    2009-01-01

    Focal adhesions (FA) are bidirectional mechanical biosensors that allow cells to integrate intracellular and extracellular cues. Their function is tightly regulated by changes in molecular composition and also by variation in the spatio-temporal dynamics of FA components within this structure. A closely regulated turnover of FA proteins within FA sites allows cells to respond appropriately to their environment, thereby impacting on cell shape and function. FA protein dynamics are linked to FA...

  15. Dynamic power flow controllers

    Science.gov (United States)

    Divan, Deepakraj M.; Prasai, Anish

    2017-03-07

    Dynamic power flow controllers are provided. A dynamic power flow controller may comprise a transformer and a power converter. The power converter is subject to low voltage stresses and not floated at line voltage. In addition, the power converter is rated at a fraction of the total power controlled. A dynamic power flow controller controls both the real and the reactive power flow between two AC sources having the same frequency. A dynamic power flow controller inserts a voltage with controllable magnitude and phase between two AC sources; thereby effecting control of active and reactive power flows between two AC sources.

  16. Controlled light exposure microscopy reveals dynamic telomere microterritories throughout the cell cycle

    NARCIS (Netherlands)

    de Vos, W.H.; Hoebe, R.A.; Joss, G.H.; Haffmans, W.; Baatout, S.; van Oostveldt, P.; Manders, E.M.M.

    2009-01-01

    Telomeres are complex end structures that confer functional integrity and positional stability to human chromosomes. Despite their critical importance, there is no clear view on telomere organization in cycling human cells and their dynamic behavior throughout the cell cycle. We investigated

  17. Dynamic modeling and predictive control in solid oxide fuel cells first principle and data-based approaches

    CERN Document Server

    Huang, Biao; Murshed, A K M Monjur

    2012-01-01

    The high temperature solid oxide fuel cell (SOFC) is identified as one of the leading fuel cell technology contenders to capture the energy market in years to come. However, in order to operate as an efficient energy generating system, the SOFC requires an appropriate control system which in turn requires a detailed modelling of process dynamics. Introducting state-of-the-art dynamic modelling, estimation, and control of SOFC systems, this book presents original modelling methods and brand new results as developed by the authors. With comprehensive coverage and bringing together many

  18. Dynamic modeling, experimental evaluation, optimal design and control of integrated fuel cell system and hybrid energy systems for building demands

    Science.gov (United States)

    Nguyen, Gia Luong Huu

    obtained experimental data, the research studied the control of airflow to regulate the temperature of reactors within the fuel processor. The dynamic model provided a platform to test the dynamic response for different control gains. With sufficient sensing and appropriate control, a rapid response to maintain the temperature of the reactor despite an increase in power was possible. The third part of the research studied the use of a fuel cell in conjunction with photovoltaic panels, and energy storage to provide electricity for buildings. This research developed an optimization framework to determine the size of each device in the hybrid energy system to satisfy the electrical demands of buildings and yield the lowest cost. The advantage of having the fuel cell with photovoltaic and energy storage was the ability to operate the fuel cell at baseload at night, thus reducing the need for large battery systems to shift the solar power produced in the day to the night. In addition, the dispatchability of the fuel cell provided an extra degree of freedom necessary for unforeseen disturbances. An operation framework based on model predictive control showed that the method is suitable for optimizing the dispatch of the hybrid energy system.

  19. Vehicle Dynamics and Control

    CERN Document Server

    Rajamani, Rajesh

    2012-01-01

    Vehicle Dynamics and Control provides a comprehensive coverage of vehicle control systems and the dynamic models used in the development of these control systems. The control system applications covered in the book include cruise control, adaptive cruise control, ABS, automated lane keeping, automated highway systems, yaw stability control, engine control, passive, active and semi-active suspensions, tire-road friction coefficient estimation, rollover prevention, and hybrid electric vehicle. In developing the dynamic model for each application, an effort is made to both keep the model simple enough for control system design but at the same time rich enough to capture the essential features of the dynamics. A special effort has been made to explain the several different tire models commonly used in literature and to interpret them physically. In the second edition of the book, chapters on roll dynamics, rollover prevention and hybrid electric vehicles have been added, and the chapter on electronic stability co...

  20. Modelling and Simulation of the Dynamics of the Antigen-Specific T Cell Response Using Variable Structure Control Theory.

    Science.gov (United States)

    Anelone, Anet J N; Spurgeon, Sarah K

    2016-01-01

    Experimental and mathematical studies in immunology have revealed that the dynamics of the programmed T cell response to vigorous infection can be conveniently modelled using a sigmoidal or a discontinuous immune response function. This paper hypothesizes strong synergies between this existing work and the dynamical behaviour of engineering systems with a variable structure control (VSC) law. These findings motivate the interpretation of the immune system as a variable structure control system. It is shown that dynamical properties as well as conditions to analytically assess the transition from health to disease can be developed for the specific T cell response from the theory of variable structure control. In particular, it is shown that the robustness properties of the specific T cell response as observed in experiments can be explained analytically using a VSC perspective. Further, the predictive capacity of the VSC framework to determine the T cell help required to overcome chronic Lymphocytic Choriomeningitis Virus (LCMV) infection is demonstrated. The findings demonstrate that studying the immune system using variable structure control theory provides a new framework for evaluating immunological dynamics and experimental observations. A modelling and simulation tool results with predictive capacity to determine how to modify the immune response to achieve healthy outcomes which may have application in drug development and vaccine design.

  1. Dynamic Thermal Model And Control Of A Pem Fuel Cell System

    DEFF Research Database (Denmark)

    Liso, Vincenzo; Nielsen, Mads Pagh

    2013-01-01

    A lumped parameter dynamic model is developed for predicting the stack performance, temperatures of the exit reactant gases and coolant liquid outlet in a proton-exchange membrane fuel cell (PEMFC) system. The air compressor, humidifier and cooling heat exchanger models are integrated to study th...

  2. GFAP isoforms control intermediate filament network dynamics, cell morphology, and focal adhesions.

    Science.gov (United States)

    Moeton, Martina; Stassen, Oscar M J A; Sluijs, Jacqueline A; van der Meer, Vincent W N; Kluivers, Liselot J; van Hoorn, Hedde; Schmidt, Thomas; Reits, Eric A J; van Strien, Miriam E; Hol, Elly M

    2016-11-01

    Glial fibrillary acidic protein (GFAP) is the characteristic intermediate filament (IF) protein in astrocytes. Expression of its main isoforms, GFAPα and GFAPδ, varies in astrocytes and astrocytoma implying a potential regulatory role in astrocyte physiology and pathology. An IF-network is a dynamic structure and has been functionally linked to cell motility, proliferation, and morphology. There is a constant exchange of IF-proteins with the network. To study differences in the dynamic properties of GFAPα and GFAPδ, we performed fluorescence recovery after photobleaching experiments on astrocytoma cells with fluorescently tagged GFAPs. Here, we show for the first time that the exchange of GFP-GFAPδ was significantly slower than the exchange of GFP-GFAPα with the IF-network. Furthermore, a collapsed IF-network, induced by GFAPδ expression, led to a further decrease in fluorescence recovery of both GFP-GFAPα and GFP-GFAPδ. This altered IF-network also changed cell morphology and the focal adhesion size, but did not alter cell migration or proliferation. Our study provides further insight into the modulation of the dynamic properties and functional consequences of the IF-network composition.

  3. PEM fuel stack dynamics, constraining supervisory control for propulsion systems in fuel cell busses

    NARCIS (Netherlands)

    Tazelaar, Edwin; Veenhuizen, Bram; Middelman, E.; Bosch, P. van den

    2013-01-01

    The last decade several prototypes of fuel cell busses have been presented [1, 2]. A closer observation of these prototypes shows remarkable differences in both sizing and control of the system components. Some busses are essentially electric vehicles with a relative low power fuel cell system used

  4. Fuel sensor-less control of a liquid feed fuel cell under dynamic loading conditions for portable power sources (I)

    Energy Technology Data Exchange (ETDEWEB)

    Chang, C.L. [Institute of Nuclear Energy Research (INER), No. 1000, Wunhua Road, Jiaan Village, Longtan Township, Taoyuan County 32546 (China); National Taiwan University (China); Chen, C.Y.; Liou, D.H. [Institute of Nuclear Energy Research (INER), No. 1000, Wunhua Road, Jiaan Village, Longtan Township, Taoyuan County 32546 (China); Sung, C.C. [National Taiwan University (China)

    2008-07-15

    This work presents a novel fuel sensor-less control scheme for a liquid feed fuel cell system that operates under dynamic loading conditions and is suitable for portable power sources. The proposed technique utilizes the operating characteristics of a fuel cell, such as voltage, current and power, to control the supply of liquid fuel and regulate its concentration. As verified by systematic experiments, this scheme controls effectively the supply of fuel under dynamic loading conditions and pushes the system toward higher power output. The primary features and advantages of sensor-less fuel control are as follows. When the fuel concentration sensor is excluded, the cost of a liquid feed fuel cell system is decreased and system volume and weight are reduced, thereby increasing specific energy density and design simplicity, and shortening system response time. Notably, temperature compensation for measurement data is unnecessary. With a decreased number of components, the control scheme improves durability and reliability of liquid feed fuel cells. These advantages will help commercialization of liquid feed fuel cells as portable power sources. (author)

  5. The Neural Mechanism Exploration of Adaptive Motor Control: Dynamical Economic Cell Allocation in the Primary Motor Cortex.

    Science.gov (United States)

    Li, Wei; Guo, Yangyang; Fan, Jing; Ma, Chaolin; Ma, Xuan; Chen, Xi; He, Jiping

    2017-05-01

    Adaptive flexibility is of significance for the smooth and efficient movements in goal attainment. However, the underlying work mechanism of the cerebral cortex in adaptive motor control still remains unclear. How does the cerebral cortex organize and coordinate the activity of a large population of cells in the implementation of various motor strategies? To explore this issue, single-unit activities from the M1 region and kinematic data were recorded simultaneously in monkeys performing 3D reach-to-grasp tasks with different perturbations. Varying motor control strategies were employed and achieved in different perturbed tasks, via the dynamic allocation of cells to modulate specific movement parameters. An economic principle was proposed for the first time to describe a basic rule for cell allocation in the primary motor cortex. This principle, defined as the Dynamic Economic Cell Allocation Mechanism (DECAM), guarantees benefit maximization in cell allocation under limited neuronal resources, and avoids committing resources to uneconomic investments for unreliable factors with no or little revenue. That is to say, the cells recruited are always preferentially allocated to those factors with reliable return; otherwise, the cells are dispatched to respond to other factors about task. The findings of this study might partially reveal the working mechanisms underlying the role of the cerebral cortex in adaptive motor control, wherein is also of significance for the design of future intelligent brain-machine interfaces and rehabilitation device.

  6. Fuel sensor-less control of a liquid feed fuel cell under dynamic loading conditions for portable power sources (II)

    Energy Technology Data Exchange (ETDEWEB)

    Chang, C.L.; Chen, C.Y.; Liou, D.H.; Chang, C.Y.; Cha, H.C. [Institute of Nuclear Energy Research (INER), No. 1000, Wunhua Rd., Jiaan Village, Longtan Township, Taoyuan County 32546 (China); Sung, C.C. [National Taiwan University (China)

    2010-03-01

    This work presents a new fuel sensor-less control scheme for liquid feed fuel cells that is able to control the supply to a fuel cell system for operation under dynamic loading conditions. The control scheme uses cell-operating characteristics, such as potential, current, and power, to regulate the fuel concentration of a liquid feed fuel cell without the need for a fuel concentration sensor. A current integral technique has been developed to calculate the quantity of fuel required at each monitoring cycle, which can be combined with the concentration regulating process to control the fuel supply for stable operation. As verified by systematic experiments, this scheme can effectively control the fuel supply of a liquid feed fuel cell with reduced response time, even under conditions where the membrane electrolyte assembly (MEA) deteriorates gradually. This advance will aid the commercialization of liquid feed fuel cells and make them more adaptable for use in portable and automotive power units such as laptops, e-bikes, and handicap cars. (author)

  7. Fractional Dynamics and Control

    CERN Document Server

    Machado, José; Luo, Albert

    2012-01-01

    Fractional Dynamics and Control provides a comprehensive overview of recent advances in the areas of nonlinear dynamics, vibration and control with analytical, numerical, and experimental results. This book provides an overview of recent discoveries in fractional control, delves into fractional variational principles and differential equations, and applies advanced techniques in fractional calculus to solving complicated mathematical and physical problems.Finally, this book also discusses the role that fractional order modeling can play in complex systems for engineering and science. Discusses how fractional dynamics and control can be used to solve nonlinear science and complexity issues Shows how fractional differential equations and models can be used to solve turbulence and wave equations in mechanics and gravity theories and Schrodinger’s equation  Presents factional relaxation modeling of dielectric materials and wave equations for dielectrics  Develops new methods for control and synchronization of...

  8. Dynamized Preparations in Cell Culture

    Directory of Open Access Journals (Sweden)

    Ellanzhiyil Surendran Sunila

    2009-01-01

    Full Text Available Although reports on the efficacy of homeopathic medicines in animal models are limited, there are even fewer reports on the in vitro action of these dynamized preparations. We have evaluated the cytotoxic activity of 30C and 200C potencies of ten dynamized medicines against Dalton's Lymphoma Ascites, Ehrlich's Ascites Carcinoma, lung fibroblast (L929 and Chinese Hamster Ovary (CHO cell lines and compared activity with their mother tinctures during short-term and long-term cell culture. The effect of dynamized medicines to induce apoptosis was also evaluated and we studied how dynamized medicines affected genes expressed during apoptosis. Mother tinctures as well as some dynamized medicines showed significant cytotoxicity to cells during short and long-term incubation. Potentiated alcohol control did not produce any cytotoxicity at concentrations studied. The dynamized medicines were found to inhibit CHO cell colony formation and thymidine uptake in L929 cells and those of Thuja, Hydrastis and Carcinosinum were found to induce apoptosis in DLA cells. Moreover, dynamized Carcinosinum was found to induce the expression of p53 while dynamized Thuja produced characteristic laddering pattern in agarose gel electrophoresis of DNA. These results indicate that dynamized medicines possess cytotoxic as well as apoptosis-inducing properties.

  9. Dynamic Long-Range Chromatin Interaction Controls Expression of IL-21 in CD4+ T Cells.

    Science.gov (United States)

    Park, Joo-Hong; Choi, Yeeun; Song, Min-Ji; Park, Keunhee; Lee, Jong-Joo; Kim, Hyoung-Pyo

    2016-05-15

    IL-21, a pleiotropic cytokine strongly linked with autoimmunity and inflammation, regulates diverse immune responses. IL-21 can be potently induced in CD4(+) T cells by IL-6; however, very little is known about the mechanisms underlying the transcriptional regulation of the Il21 gene at the chromatin level. In this study, we demonstrated that a conserved noncoding sequence located 49 kb upstream of the Il21 gene contains an enhancer element that can upregulate Il21 gene expression in a STAT3- and NFAT-dependent manner. Additionally, we identified enhancer-blocking insulator elements in the Il21 locus, which constitutively bind CTCF and cohesin. In naive CD4(+) T cells, these upstream and downstream CTCF binding sites interact with each other to make a DNA loop; however, the Il21 promoter does not interact with any cis-elements in the Il21 locus. In contrast, stimulation of CD4(+) T cells with IL-6 leads to recruitment of STAT3 to the promoter and novel distal enhancer region. This induces dynamic changes in chromatin configuration, bringing the promoter and the regulatory elements in close spatial proximity. The long-range interaction between the promoter and distal enhancer region was dependent on IL-6/STAT3 signaling pathway but was disrupted in regulatory T cells, where IL-21 expression was repressed. Thus, our work uncovers a novel topological chromatin framework underlying proper transcriptional regulation of the Il21 gene.

  10. Dynamic water management of polymer electrolyte membrane fuel cells using intermittent RH control

    Science.gov (United States)

    Hussaini, I. S.; Wang, C. Y.

    A novel method of water management of polymer electrolyte membrane (PEM) fuel cells using intermittent humidification is presented in this study. The goal is to maintain the membrane close to full humidification, while eliminating channel flooding. The entire cycle is divided into four stages: saturation and de-saturation of the gas diffusion layer followed by de-hydration and hydration of membrane. By controlling the duration of dry and humid flows, it is shown that the cell voltage can be maintained within a narrow band. The technique is applied on experimental test cells using both plain and hydrophobic materials for the gas diffusion layer and an improvement in performance as compared to steady humidification is demonstrated. Duration of dry and humid flows is determined experimentally for several operating conditions.

  11. Dynamic water management of polymer electrolyte membrane fuel cells using intermittent RH control

    KAUST Repository

    Hussaini, I.S.

    2010-06-01

    A novel method of water management of polymer electrolyte membrane (PEM) fuel cells using intermittent humidification is presented in this study. The goal is to maintain the membrane close to full humidification, while eliminating channel flooding. The entire cycle is divided into four stages: saturation and de-saturation of the gas diffusion layer followed by de-hydration and hydration of membrane. By controlling the duration of dry and humid flows, it is shown that the cell voltage can be maintained within a narrow band. The technique is applied on experimental test cells using both plain and hydrophobic materials for the gas diffusion layer and an improvement in performance as compared to steady humidification is demonstrated. Duration of dry and humid flows is determined experimentally for several operating conditions. © 2010 Elsevier B.V. All rights reserved.

  12. Dual control cell reaction ensemble molecular dynamics: A method for simulations of reactions and adsorption in porous materials

    Science.gov (United States)

    Lísal, Martin; Brennan, John K.; Smith, William R.; Siperstein, Flor R.

    2004-09-01

    We present a simulation tool to study fluid mixtures that are simultaneously chemically reacting and adsorbing in a porous material. The method is a combination of the reaction ensemble Monte Carlo method and the dual control volume grand canonical molecular dynamics technique. The method, termed the dual control cell reaction ensemble molecular dynamics method, allows for the calculation of both equilibrium and nonequilibrium transport properties in porous materials such as diffusion coefficients, permeability, and mass flux. Control cells, which are in direct physical contact with the porous solid, are used to maintain the desired reaction and flow conditions for the system. The simulation setup closely mimics an actual experimental system in which the thermodynamic and flow parameters are precisely controlled. We present an application of the method to the dry reforming of methane reaction within a nanoscale reactor model in the presence of a semipermeable membrane that was modeled as a porous material similar to silicalite. We studied the effects of the membrane structure and porosity on the reaction species permeability by considering three different membrane models. We also studied the effects of an imposed pressure gradient across the membrane on the mass flux of the reaction species. Conversion of syngas (H2/CO) increased significantly in all the nanoscale membrane reactor models considered. A brief discussion of further potential applications is also presented.

  13. Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells.

    Science.gov (United States)

    Niopek, Dominik; Benzinger, Dirk; Roensch, Julia; Draebing, Thomas; Wehler, Pierre; Eils, Roland; Di Ventura, Barbara

    2014-07-14

    The function of many eukaryotic proteins is regulated by highly dynamic changes in their nucleocytoplasmic distribution. The ability to precisely and reversibly control nuclear translocation would, therefore, allow dissecting and engineering cellular networks. Here we develop a genetically encoded, light-inducible nuclear localization signal (LINuS) based on the LOV2 domain of Avena sativa phototropin 1. LINuS is a small, versatile tag, customizable for different proteins and cell types. LINuS-mediated nuclear import is fast and reversible, and can be tuned at different levels, for instance, by introducing mutations that alter AsLOV2 domain photo-caging properties or by selecting nuclear localization signals (NLSs) of various strengths. We demonstrate the utility of LINuS in mammalian cells by controlling gene expression and entry into mitosis with blue light.

  14. Optimizing the dynamics of a two-cell DC-DC buck converter by time delayed feedback control

    Science.gov (United States)

    Feki, M.; El Aroudi, A.; Robert, B. G. M.; Martínez-Salamero, L.

    2011-11-01

    A study of the dynamical behavior of a two-cell DC-DC buck converter under a digital time delayed feedback control (TDFC) is presented. Various numerical simulations and dynamical aspects of this system are illustrated in the time domain and in the parameter space. Without TDFC, the system may present many undesirable behaviors such as sub-harmonics and chaotic oscillations. TDFC is able to widen the stability range of the system. Optimum values of parameters giving rise to fast response while maintaining stable periodic behavior are given in closed form. However, it is detected that in a certain region of the parameter space, the stabilized periodic orbit may coexist with a chaotic attractor. Boundary between basins of attraction are obtained by means of numerical simulations.

  15. Hippo pathway effectors control cardiac progenitor cell fate by acting as dynamic sensors of substrate mechanics and nanostructure

    KAUST Repository

    Mosqueira, Diogo

    2014-03-25

    Stem cell responsiveness to extracellular matrix (ECM) composition and mechanical cues has been the subject of a number of investigations so far, yet the molecular mechanisms underlying stem cell mechano-biology still need full clarification. Here we demonstrate that the paralog proteins YAP and TAZ exert a crucial role in adult cardiac progenitor cell mechano-sensing and fate decision. Cardiac progenitors respond to dynamic modifications in substrate rigidity and nanopattern by promptly changing YAP/TAZ intracellular localization. We identify a novel activity of YAP and TAZ in the regulation of tubulogenesis in 3D environments and highlight a role for YAP/TAZ in cardiac progenitor proliferation and differentiation. Furthermore, we show that YAP/TAZ expression is triggered in the heart cells located at the infarct border zone. Our results suggest a fundamental role for the YAP/TAZ axis in the response of resident progenitor cells to the modifications in microenvironment nanostructure and mechanics, thereby contributing to the maintenance of myocardial homeostasis in the adult heart. These proteins are indicated as potential targets to control cardiac progenitor cell fate by materials design. © 2014 American Chemical Society.

  16. Hippo pathway effectors control cardiac progenitor cell fate by acting as dynamic sensors of substrate mechanics and nanostructure.

    Science.gov (United States)

    Mosqueira, Diogo; Pagliari, Stefania; Uto, Koichiro; Ebara, Mitsuhiro; Romanazzo, Sara; Escobedo-Lucea, Carmen; Nakanishi, Jun; Taniguchi, Akiyoshi; Franzese, Ornella; Di Nardo, Paolo; Goumans, Marie José; Traversa, Enrico; Pinto-do-Ó, Perpetua; Aoyagi, Takao; Forte, Giancarlo

    2014-03-25

    Stem cell responsiveness to extracellular matrix (ECM) composition and mechanical cues has been the subject of a number of investigations so far, yet the molecular mechanisms underlying stem cell mechano-biology still need full clarification. Here we demonstrate that the paralog proteins YAP and TAZ exert a crucial role in adult cardiac progenitor cell mechano-sensing and fate decision. Cardiac progenitors respond to dynamic modifications in substrate rigidity and nanopattern by promptly changing YAP/TAZ intracellular localization. We identify a novel activity of YAP and TAZ in the regulation of tubulogenesis in 3D environments and highlight a role for YAP/TAZ in cardiac progenitor proliferation and differentiation. Furthermore, we show that YAP/TAZ expression is triggered in the heart cells located at the infarct border zone. Our results suggest a fundamental role for the YAP/TAZ axis in the response of resident progenitor cells to the modifications in microenvironment nanostructure and mechanics, thereby contributing to the maintenance of myocardial homeostasis in the adult heart. These proteins are indicated as potential targets to control cardiac progenitor cell fate by materials design.

  17. Dynamic PID loop control

    Energy Technology Data Exchange (ETDEWEB)

    Pei, L.; Klebaner, A.; Theilacker, J.; Soyars, W.; Martinez, A.; Bossert, R.; DeGraff, B.; Darve, C.; /Fermilab

    2011-06-01

    The Horizontal Test Stand (HTS) SRF Cavity and Cryomodule 1 (CM1) of eight 9-cell, 1.3GHz SRF cavities are operating at Fermilab. For the cryogenic control system, how to hold liquid level constant in the cryostat by regulation of its Joule-Thompson JT-valve is very important after cryostat cool down to 2.0 K. The 72-cell cryostat liquid level response generally takes a long time delay after regulating its JT-valve; therefore, typical PID control loop should result in some cryostat parameter oscillations. This paper presents a type of PID parameter self-optimal and Time-Delay control method used to reduce cryogenic system parameters oscillation.

  18. Dynamic PID loop control

    CERN Document Server

    Pei, L; Theilacker, J; Soyars, W; Martinez, A; Bossert, R; DeGraff, B; Darve, C

    2012-01-01

    The Horizontal Test Stand (HTS) SRF Cavity and Cryomodule 1 (CM1) of eight 9-cell, 1.3GHz SRF cavities are operating at Fermilab. For the cryogenic control system, how to hold liquid level constant in the cryostat by regulation of its Joule-Thompson JT-valve is very important after cryostat cool down to 2.0 K. The 72-cell cryostat liquid level response generally takes a long time delay after regulating its JT-valve; therefore, typical PID control loop should result in some cryostat parameter oscillations. This paper presents a type of PID parameter self-optimal and Time-Delay control method used to reduce cryogenic system parameters' oscillation.

  19. Dynamics of cell orientation

    Science.gov (United States)

    de, Rumi; Zemel, Assaf; Safran, Samuel A.

    2007-09-01

    Many physiological processes depend on the response of biological cells to mechanical forces generated by the contractile activity of the cell or by external stresses. Using a simple theoretical model that includes the forces due to both the mechanosensitivity of cells and the elasticity of the matrix, we predict the dynamics and orientation of cells in both the absence and presence of applied stresses. The model predicts many features observed in measurements of cellular forces and orientation including the increase with time of the cellular forces in the absence of applied stress and the consequent decrease of the force in the presence of quasi-static stresses. We also explain the puzzling observation of parallel alignment of cells for static and quasi-static stresses and of nearly perpendicular alignment for dynamically varying stresses. In addition, we predict the response of the cellular orientation to a sinusoidally varying applied stress as a function of frequency.

  20. Dynamics of immigration control.

    Science.gov (United States)

    Djajic, S

    1999-01-01

    This paper examines the dynamic implications of US border control policies and internal enforcement measures for the pattern of illegal immigration and the sectoral allocation of clandestine foreign workers. Efforts to counteract illegal immigration into the US have been increasing steadily following the passage of the Immigration Reform and Control Act of 1986. The purpose of the Act is to reduce illegal immigration with the aid of three instruments: 1) employer sanctions; 2) increased controls along the border; and 3) a legalization program designed to meet the existing demand for agricultural labor in geographic locations that are in proximity of Mexico, the principal source of clandestine foreign labor. The effect of tougher border control measures increases the cost of illegal entry, discouraging clandestine inflows. On the other hand, these measures induce migrants to increase their own anti-detection efforts, reducing the probability of detection and the deportation rate. If the latter effect should dominate, the steady-state stock of clandestine foreign labor will actually increase in response to more vigorous border control measures. Explicit consideration of the role of networks in the clandestine labor market suggests the need for a drastic policy change. This policy change should target illegal migration in areas with high concentrations of clandestine foreign workers. Complementary measures should accompany this policy change to prevent unbalanced enforcement measures.

  1. Single cell dynamic phenotyping

    OpenAIRE

    Katherin Patsch; Chi-Li Chiu; Mark Engeln; Agus, David B.; Parag Mallick; Shannon M. Mumenthaler; Daniel Ruderman

    2016-01-01

    Live cell imaging has improved our ability to measure phenotypic heterogeneity. However, bottlenecks in imaging and image processing often make it difficult to differentiate interesting biological behavior from technical artifact. Thus there is a need for new methods that improve data quality without sacrificing throughput. Here we present a 3-step workflow to improve dynamic phenotype measurements of heterogeneous cell populations. We provide guidelines for image acquisition, phenotype track...

  2. Extracellular pH dynamically controls cell surface delivery of functional TRPV5 channels.

    NARCIS (Netherlands)

    Lambers, T.T.; Oancea, E.; Groot, T. de; Topala, C.N.; Hoenderop, J.G.J.; Bindels, R.J.M.

    2007-01-01

    Extracellular pH has long been known to affect the rate and magnitude of ion transport processes among others via regulation of ion channel activity. The Ca(2+)-selective transient receptor potential vanilloid 5 (TRPV5) channel constitutes the apical entry gate in Ca(2+)-transporting cells, contribu

  3. On the dynamics of StemBells: Microbubble-conjugated stem cells for ultrasound-controlled delivery

    Science.gov (United States)

    Kokhuis, Tom J. A.; Naaijkens, Benno A.; Juffermans, Lynda J. M.; Kamp, Otto; van der Steen, Antonius F. W.; Versluis, Michel; de Jong, Nico

    2017-07-01

    The use of stem cells for regenerative tissue repair is promising but hampered by the low number of cells delivered to the site of injury. To increase the delivery, we propose a technique in which stem cells are linked to functionalized microbubbles, creating echogenic complex dubbed StemBells. StemBells are highly susceptible to acoustic radiation force which can be employed after injection to push the StemBells locally to the treatment site. To optimally benefit from the delivery technique, a thorough characterization of the dynamics of StemBells during ultrasound exposure is needed. Using high-speed optical imaging, we study the dynamics of StemBells as a function of the applied frequency from which resonance curves were constructed. A theoretical model, based on a modified Rayleigh-Plesset type equation, captured the experimental resonance characteristics and radial dynamics in detail.

  4. Spacecraft attitude dynamics and control

    Science.gov (United States)

    Chobotov, Vladimir A.

    This overview of spacecraft dynamics encompasses the fundamentals of kinematics, rigid-body dynamics, linear control theory, orbital environmental effects, and the stability of motion. The theoretical treatment of each issue is complemented by specific references to spacecraft control systems based on spin, dual-spin, three-axis-active, and reaction-wheel methodologies. Also examined are control-moment-gyro, gravity-gradient, and magnetic control systems with attention given to key issues such as nutation damping, separation dynamics of spinning bodies, and tethers. Environmental effects that impinge on the application of spacecraft-attitude dynamics are shown to be important, and consideration is given to gravitation, solar radiation, aerodynamics, and geomagnetics. The publication gives analytical methods for examining the practical implementation of the control techniques as they apply to spacecraft.

  5. Controlling Chemistry in Dynamic Nanoscale Systems

    DEFF Research Database (Denmark)

    Jesorka, Aldo; Lizana, Ludvig; Konkoli, Zoran

    2011-01-01

    Spatial organization and shape dynamics are inherent properties of biological cells and cell interiors. There are strong indications that these features are important for the in vivo control of reaction parameters in biochemical transformations. Nanofluidic model devices founded on surfactant...... of the concept. Controlled release of chol-DNA molecules from SU-8 surfaces gives the possibility to dynamically change surface and/or solution properties in micro and nanoreactor applications, opening access to stable 2D chemistry on surface-based devices with potential for easy interfacing with conventional...

  6. SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae

    DEFF Research Database (Denmark)

    Garcia Vanegas, Katherina; Lehka, Beata Joanna; Mortensen, Uffe Hasbro

    2017-01-01

    to the pathway control state where production was optimized by downregulating an essential gene TSC13, hence, reducing formation of a byproduct. Conclusions We have successfully integrated two CRISPR tools, one for genetic engineering and one for pathway control, into one system and successfully used it for cell...

  7. SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae

    DEFF Research Database (Denmark)

    Garcia Vanegas, Katherina; Lehka, Beata Joanna; Mortensen, Uffe Hasbro

    2017-01-01

    to the pathway control state where production was optimized by downregulating an essential gene TSC13, hence, reducing formation of a byproduct. Conclusions We have successfully integrated two CRISPR tools, one for genetic engineering and one for pathway control, into one system and successfully used it for cell...

  8. Microbial Cell Dynamics Lab (MCDL)

    Data.gov (United States)

    Federal Laboratory Consortium — The Microbial Cell Dynamics Laboratory at PNNL enables scientists to study the molecular details of microbes under relevant environmental conditions. The MCDL seeks...

  9. Optimal Control of Evolutionary Dynamics

    CERN Document Server

    Chakrabarti, Raj; McLendon, George

    2008-01-01

    Elucidating the fitness measures optimized during the evolution of complex biological systems is a major challenge in evolutionary theory. We present experimental evidence and an analytical framework demonstrating how biochemical networks exploit optimal control strategies in their evolutionary dynamics. Optimal control theory explains a striking pattern of extremization in the redox potentials of electron transport proteins, assuming only that their fitness measure is a control objective functional with bounded controls.

  10. Stochastic dynamics and control

    CERN Document Server

    Sun, Jian-Qiao; Zaslavsky, George

    2006-01-01

    This book is a result of many years of author's research and teaching on random vibration and control. It was used as lecture notes for a graduate course. It provides a systematic review of theory of probability, stochastic processes, and stochastic calculus. The feedback control is also reviewed in the book. Random vibration analyses of SDOF, MDOF and continuous structural systems are presented in a pedagogical order. The application of the random vibration theory to reliability and fatigue analysis is also discussed. Recent research results on fatigue analysis of non-Gaussian stress proc

  11. SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae.

    Science.gov (United States)

    Vanegas, Katherina García; Lehka, Beata Joanna; Mortensen, Uffe Hasbro

    2017-02-08

    The yeast Saccharomyces cerevisiae is increasingly used as a cell factory. However, cell factory construction time is a major obstacle towards using yeast for bio-production. Hence, tools to speed up cell factory construction are desirable. In this study, we have developed a new Cas9/dCas9 based system, SWITCH, which allows Saccharomyces cerevisiae strains to iteratively alternate between a genetic engineering state and a pathway control state. Since Cas9 induced recombination events are crucial for SWITCH efficiency, we first developed a technique TAPE, which we have successfully used to address protospacer efficiency. As proof of concept of the use of SWITCH in cell factory construction, we have exploited the genetic engineering state of a SWITCH strain to insert the five genes necessary for naringenin production. Next, the naringenin cell factory was switched to the pathway control state where production was optimized by downregulating an essential gene TSC13, hence, reducing formation of a byproduct. We have successfully integrated two CRISPR tools, one for genetic engineering and one for pathway control, into one system and successfully used it for cell factory construction.

  12. Manual for Dynamic Triaxial Cell

    DEFF Research Database (Denmark)

    Pedersen, Thomas Schmidt; Ibsen, Lars Bo

    This report is a test report that describes the test setup for a dynamic triaxial cell at the Laboratory for Geotechnique at Aalborg University.......This report is a test report that describes the test setup for a dynamic triaxial cell at the Laboratory for Geotechnique at Aalborg University....

  13. Workload control dynamics in practice

    NARCIS (Netherlands)

    Soepenberg, G. D.; Land, M. J.; Gaalman, G. J. C.

    2012-01-01

    Workload control (WLC) is a well established production planning and control concept for make-to-order companies. The main insights into WLC are being gained from stationary simulation studies. Knowledge on its functioning in practice is limited, especially in a dynamic setting. The aim of this pape

  14. Cell control report

    CERN Document Server

    2013-01-01

    Please note this is a Short Discount publication. This extensive report provides an essential overview of cells and their use as factory automation building blocks. The following issues are discussed in depth: Cell integration Cell software and standards Future technologies applied to cells Plus Cell control applications including: - rotary parts manufacturing - diesel engine component development - general cell control development at the General Electric Corporation - a vendor list.

  15. Epigenetic dynamics across the cell cycle

    DEFF Research Database (Denmark)

    Kheir, Tony Bou; Lund, Anders H.

    2010-01-01

    Progression of the mammalian cell cycle depends on correct timing and co-ordination of a series of events, which are managed by the cellular transcriptional machinery and epigenetic mechanisms governing genome accessibility. Epigenetic chromatin modifications are dynamic across the cell cycle......, and are shown to influence and be influenced by cell-cycle progression. Chromatin modifiers regulate cell-cycle progression locally by controlling the expression of individual genes and globally by controlling chromatin condensation and chromosome segregation. The cell cycle, on the other hand, ensures...... a correct inheritance of epigenetic chromatin modifications to daughter cells. In this chapter, we summarize the current knowledge on the dynamics of epigenetic chromatin modifications during progression of the cell cycle....

  16. Traffic jams: dynamics and control.

    Science.gov (United States)

    Orosz, Gábor; Wilson, R Eddie; Stépán, Gábor

    2010-10-13

    This introductory paper reviews the current state-of-the-art scientific methods used for modelling, analysing and controlling the dynamics of vehicular traffic. Possible mechanisms underlying traffic jam formation and propagation are presented from a dynamical viewpoint. Stable and unstable motions are described that may give the skeleton of traffic dynamics, and the effects of driver behaviour are emphasized in determining the emergent state in a vehicular system. At appropriate points, references are provided to the papers published in the corresponding Theme Issue.

  17. Conformational Dynamics of the Focal Adhesion Targeting Domain Control Specific Functions of Focal Adhesion Kinase in Cells

    KAUST Repository

    Kadaré, Gress

    2015-01-02

    Focal adhesion (FA) kinase (FAK) regulates cell survival and motility by transducing signals from membrane receptors. The C-terminal FA targeting (FAT) domain of FAK fulfils multiple functions, including recruitment to FAs through paxillin binding. Phosphorylation of FAT on Tyr925 facilitates FA disassembly and connects to the MAPK pathway through Grb2 association, but requires dissociation of the first helix (H1) of the four-helix bundle of FAT. We investigated the importance of H1 opening in cells by comparing the properties of FAK molecules containing wild-type or mutated FAT with impaired or facilitated H1 openings. These mutations did not alter the activation of FAK, but selectively affected its cellular functions, including self-association, Tyr925 phosphorylation, paxillin binding, and FA targeting and turnover. Phosphorylation of Tyr861, located between the kinase and FAT domains, was also enhanced by the mutation that opened the FAT bundle. Similarly phosphorylation of Ser910 by ERK in response to bombesin was increased by FAT opening. Although FAK molecules with the mutation favoring FAT opening were poorly recruited at FAs, they efficiently restored FA turnover and cell shape in FAK-deficient cells. In contrast, the mutation preventing H1 opening markedly impaired FAK function. Our data support the biological importance of conformational dynamics of the FAT domain and its functional interactions with other parts of the molecule.

  18. Biped control via nonlinear dynamics

    Science.gov (United States)

    Hmam, Hatem M.

    1992-09-01

    This thesis applies nonlinear techniques to actuate a biped system and provides a rigorous analysis of the resulting motion. From observation of human locomotion, it is believed that the 'complex' dynamics developed by the aggregation of multiple muscle systems can be generated by a reduced order system which captures the rough details of the locomotion process. The investigation is begun with a simple model of a biped system. Since the locomotion process is cyclic in nature, we focus on applying the topologically similar concept of limit cycles to the simple model in order to generate the desired gaits. A rigorous analysis of the biped dynamics shows that the controlled motion is robust against dynamical disturbances. In addition, different biped gaits are generated by merely adjusting some of the limit cycle parameters. More dynamical and actuation complexities are then added for realism. First, two small foot components are added and the overall biped motion under the same control actuation is analyzed. Due to the physical constraints on the feet, it is shown using singular perturbation theory how the gross behavior of the biped dynamics are dictated by those of the reduced model. Next, an analysis of the biped dynamics under added nonlinear elasticities in the legs is carried out. Moreover, using a slightly modified model, forward motion is generated in the sagittal plane. At each step, a small amount of energy is consistently derived from the vertical plane and converted into a forward motion. Stability of the forward dynamics is guaranteed by appropriate foot placement. Finally, the robustness of the controlled biped dynamics is rigorously analyzed and illustrated through extensive computer simulations.

  19. Dynamic Controllability and Dispatchability Relationships

    Science.gov (United States)

    Morris, Paul Henry

    2014-01-01

    An important issue for temporal planners is the ability to handle temporal uncertainty. Recent papers have addressed the question of how to tell whether a temporal network is Dynamically Controllable, i.e., whether the temporal requirements are feasible in the light of uncertain durations of some processes. We present a fast algorithm for Dynamic Controllability. We also note a correspondence between the reduction steps in the algorithm and the operations involved in converting the projections to dispatchable form. This has implications for the complexity for sparse networks.

  20. Theory of controlled quantum dynamics

    CERN Document Server

    De Martino, S; Illuminati, F; Martino, Salvatore De; Siena, Silvio De; Illuminati, Fabrizio

    1997-01-01

    We introduce a general formalism, based on the stochastic formulation of quantum mechanics, to obtain localized quasi-classical wave packets as dynamically controlled systems, for arbitrary anharmonic potentials. The control is in general linear, and it amounts to introduce additional quadratic and linear time-dependent terms to the given potential. In this way one can construct for general systems either coherent packets moving with constant dispersion, or dynamically squeezed packets whose spreading remains bounded for all times. In the standard operatorial framework our scheme corresponds to a suitable generalization of the displacement and scaling operators that generate the coherent and squeezed states of the harmonic oscillator.

  1. Real-time imaging of bHLH transcriptional factors reveals their dynamic control in the multipotency and fate choice of neural stem cells

    Directory of Open Access Journals (Sweden)

    Itaru eImayoshi

    2015-08-01

    Full Text Available The basic-helix-loop-helix (bHLH transcription factors Ascl1/Mash1, Hes1, and Olig2 regulate the fate choice of neurons, astrocytes, and oligodendrocytes, respectively; however, these factors are coexpressed in self-renewing multipotent neural stem cells (NSCs even before cell fate determination. This fact raises the possibility these fate determination factors are differentially expressed between self-renewing and differentiating NSCs with unique expression dynamics. Real-time imaging analysis utilizing fluorescent proteins is a powerful strategy for monitoring expression dynamics. Fusion with fluorescent reporters makes it possible to analyze the dynamic behavior of specific proteins in living cells. However, it is technically challenging to conduct long-term imaging of proteins, particularly those with low expression levels, because a high-sensitivity and low-noise imaging system is required, and very often bleaching of fluorescent proteins and cell toxicity by prolonged laser exposure are problematic. Furthermore, to analyze the functional roles of the dynamic expression of cellular proteins, it is essential to image reporter fusion proteins that are expressed at comparable levels to their endogenous expression. In this review, we introduce our recent reports about the dynamic control of bHLH transcription factors in multipotency and fate choice of NSCs, focusing on real-time imaging of fluorescent reporters fused with bHLH transcription factors. Our imaging results indicate that bHLH transcription factors are expressed in an oscillatory manner by NSCs, and that one of them becomes dominant during fate choice. We propose that the multipotent state of NSCs correlates with the oscillatory expression of several bHLH transcription factors, whereas the differentiated state correlates with the sustained expression of a single bHLH transcription factor.

  2. Real-time imaging of bHLH transcription factors reveals their dynamic control in the multipotency and fate choice of neural stem cells.

    Science.gov (United States)

    Imayoshi, Itaru; Ishidate, Fumiyoshi; Kageyama, Ryoichiro

    2015-01-01

    The basic-helix-loop-helix (bHLH) transcription factors Ascl1/Mash1, Hes1, and Olig2 regulate the fate choice of neurons, astrocytes, and oligodendrocytes, respectively; however, these factors are coexpressed in self-renewing multipotent neural stem cells (NSCs) even before cell fate determination. This fact raises the possibility that these fate determination factors are differentially expressed between self-renewing and differentiating NSCs with unique expression dynamics. Real-time imaging analysis utilizing fluorescent proteins is a powerful strategy for monitoring expression dynamics. Fusion with fluorescent reporters makes it possible to analyze the dynamic behavior of specific proteins in living cells. However, it is technically challenging to conduct long-term imaging of proteins, particularly those with low expression levels, because a high-sensitivity and low-noise imaging system is required, and very often bleaching of fluorescent proteins and cell toxicity by prolonged laser exposure are problematic. Furthermore, to analyze the functional roles of the dynamic expression of cellular proteins, it is essential to image reporter fusion proteins that are expressed at comparable levels to their endogenous expression. In this review, we introduce our recent reports about the dynamic control of bHLH transcription factors in multipotency and fate choice of NSCs, focusing on real-time imaging of fluorescent reporters fused with bHLH transcription factors. Our imaging results indicate that bHLH transcription factors are expressed in an oscillatory manner by NSCs, and that one of them becomes dominant during fate choice. We propose that the multipotent state of NSCs correlates with the oscillatory expression of several bHLH transcription factors, whereas the differentiated state correlates with the sustained expression of a single bHLH transcription factor.

  3. Voltage Controlled Dynamic Demand Response

    DEFF Research Database (Denmark)

    Bhattarai, Bishnu Prasad; Bak-Jensen, Birgitte; Mahat, Pukar

    2013-01-01

    . An adaptive dynamic model has been developed to determine composite voltage dependency of an aggregated load on feeder level. Following the demand dispatch or control signal, optimum voltage setting at the LV substation is determined based on the voltage dependency of the load. Furthermore, a new technique...

  4. Control of high affinity interactions in the talin C terminus: how talin domains coordinate protein dynamics in cell adhesions.

    Science.gov (United States)

    Himmel, Mirko; Ritter, Anett; Rothemund, Sven; Pauling, Björg V; Rottner, Klemens; Gingras, Alexandre R; Ziegler, Wolfgang H

    2009-05-15

    In cell-extracellular matrix junctions (focal adhesions), the cytoskeletal protein talin is central to the connection of integrins to the actin cytoskeleton. Talin is thought to mediate this connection via its two integrin, (at least) three actin, and several vinculin binding sites. The binding sites are cryptic in the head-to-rod autoinhibited cytoplasmic form of the protein and require (stepwise) conformational activation. This activation process, however, remains poorly understood, and there are contradictory models with respect to the determinants of adhesion site localization. Here, we report turnover rates and protein-protein interactions in a range of talin rod domain constructs varying in helix bundle structure. We conclude that several bundles of the C terminus cooperate to regulate targeting and concomitantly tailor high affinity interactions of the talin rod in cell adhesions. Intrinsic control of ligand binding activities is essential for the coordination of adhesion site function of talin.

  5. Controlling dynamics in diatomic systems

    Indian Academy of Sciences (India)

    Praveen Kumar; Harjinder Singh

    2007-09-01

    Controlling molecular energetics using laser pulses is exemplified for nuclear motion in two different diatomic systems. The problem of finding the optimized field for maximizing a desired quantum dynamical target is formulated using an iterative method. The method is applied for two diatomic systems, HF and OH. The power spectra of the fields and evolution of populations of different vibrational states during transitions are obtained.

  6. Theory of controlled quantum dynamics

    Energy Technology Data Exchange (ETDEWEB)

    De Martino, Salvatore; De Siena, Silvio; Illuminati, Fabrizio [Dipartimento di Fisica, Universita di Salerno, and INFN, Sezione di Napoli, Gruppo collegato di Salerno, Baronissi (Italy)

    1997-06-07

    We introduce a general formalism to obtain localized quantum wavepackets as dynamically controlled systems, in the framework of Nelson stochastic quantization. We show that in general the control is linear, and it amounts to introducing additional time-dependent terms in the potential. In this way one can construct for general systems either coherent packets following classical motion with constant dispersion, or coherent packets following classical motion whose time-dependent dispersion remains bounded for all times. We show that in the operatorial language our scheme amounts to introducing a suitable generalization to arbitrary potentials of the displacement and scaling operators that generate the coherent and squeezed states of the harmonic oscillator. (author)

  7. Dynamic trajectory control of gliders

    CERN Document Server

    Dilão, Rui

    2013-01-01

    We propose a new dynamic control algorithm in order to direct the trajectory of a glider to a pre-assigned target point. The algorithms runs iteratively and the approach to the target point is self-correcting. The algorithm is applicable to any non-powered lift-enabled vehicle (glider) travelling in planetary atmospheres. As a proof of concept, we have applied the new algorithm to the command and control of the trajectory of the Space Shuttle during the Terminal Area Energy Management (TAEM) phase.

  8. Cell Control Engineering

    DEFF Research Database (Denmark)

    Lynggaard, Hans Jørgen Birk; Alting, Leo

    1996-01-01

    The engineering process of creating cell control systems is described, and a Cell Control Engineering (CCE) concept is defined. The purpose is to assist people, representing different disciplines in the organisation, to implement cell controllers by addressing the complexity of having many systems...... in physically and logically different and changing manufacturing environments. The defined CCE concept combines state-of-the-art of commercially available enabling technologies for automation system software development, generic cell control models and guidelines for the complete engineering process....... It facilitates the understanding of the task and structure of cell controllers and uses this knowledge directly in the implementation of the system. By applying generic models CCE facilitates reuse of software components and maintenance of applications. In many enterprises, software makes up an increasing part...

  9. Transcription Dynamics in Living Cells.

    Science.gov (United States)

    Lenstra, Tineke L; Rodriguez, Joseph; Chen, Huimin; Larson, Daniel R

    2016-07-01

    The transcription cycle can be roughly divided into three stages: initiation, elongation, and termination. Understanding the molecular events that regulate all these stages requires a dynamic view of the underlying processes. The development of techniques to visualize and quantify transcription in single living cells has been essential in revealing the transcription kinetics. They have revealed that (a) transcription is heterogeneous between cells and (b) transcription can be discontinuous within a cell. In this review, we discuss the progress in our quantitative understanding of transcription dynamics in living cells, focusing on all parts of the transcription cycle. We present the techniques allowing for single-cell transcription measurements, review evidence from different organisms, and discuss how these experiments have broadened our mechanistic understanding of transcription regulation.

  10. 质子交换膜燃料电池电堆的动态热模型及其温度控制%Dynamic Thermal Model and Temperature Control of Proton Exchange Membrane Fuel Cell Stack

    Institute of Scientific and Technical Information of China (English)

    邵庆龙; 卫东; 曹广益; 朱新坚

    2005-01-01

    A dynamic thermal transfer model of a proton exchange membrane fuel cell (PEMFC) stack is developed based on energy conservation in order to reach better temperature control of PEMFC stack. Considering its uncertain parameters and disturbance, we propose a robust adaptive controller based on backstepping algorithm of Lyaponov function. Numerical simulations indicate the validity of the proposed controller.

  11. Development of a new type of high pressure calorimetric cell, mechanically agitated and equipped with a dynamic pressure control system: Application to the characterization of gas hydrates

    Energy Technology Data Exchange (ETDEWEB)

    Plantier, F., E-mail: frederic.plantier@univ-pau.fr; Missima, D.; Torré, J.-P. [Univ Pau and Pays Adour, CNRS, TOTAL - UMR 5150 – LFC-R - Laboratoire des Fluides Complexes et leurs Réservoirs, BP 1155 – PAU, F-64013 (France); Marlin, L. [Univ Pau and Pays Adour, IPRA FR2952 - Fédération de Recherche- Atelier de Physique Générale, BP 1155 – PAU, F-64013 (France)

    2013-12-15

    A novel prototype of calorimetric cell has been developed allowing experiments under pressure with an in situ agitation system and a dynamic control of the pressure inside the cell. The use of such a system opens a wide range of potential practical applications for determining properties of complex fluids in both pressurized and agitated conditions. The technical details of this prototype and its calibration procedure are described, and an application devoted to the determination of phase equilibrium and phase change enthalpy of gas hydrates is presented. Our results, obtained with a good precision and reproducibility, were found in fairly good agreement with those found in literature, illustrate the various interests to use this novel apparatus.

  12. The Statistical Dynamics of Nonequilibrium Control

    Science.gov (United States)

    Rotskoff, Grant Murray

    Living systems, even at the scale of single molecules, are constantly adapting to changing environmental conditions. The physical response of a nanoscale system to external gradients or changing thermodynamic conditions can be chaotic, nonlinear, and hence difficult to control or predict. Nevertheless, biology has evolved systems that reliably carry out the cell's vital functions efficiently enough to ensure survival. Moreover, the development of new experimental techniques to monitor and manipulate single biological molecules has provided a natural testbed for theoretical investigations of nonequilibrium dynamics. This work focuses on developing paradigms for both understanding the principles of nonequilibrium dynamics and also for controlling such systems in the presence of thermal fluctuations. Throughout this work, I rely on a perspective based on two central ideas in nonequilibrium statistical mechanics: large deviation theory, which provides a formalism akin to thermodynamics for nonequilibrium systems, and the fluctuation theorems which identify time symmetry breaking with entropy production. I use the tools of large deviation theory to explore concepts like efficiency and optimal coarse-graining in microscopic dynamical systems. The results point to the extreme importance of rare events in nonequilibrium dynamics. In the context of rare dynamical events, I outline a formal approach to predict efficient control protocols for nonequilibrium systems and develop computational tools to solve the resulting high dimensional optimization problems. The final chapters of this work focus on applications to self-assembly dynamics. I show that the yield of desired structures can be enhanced by driving a system away from equilibrium, using analysis inspired by the theory of the hydrophobic effect. Finally, I demonstrate that nanoscale, protein shells can be modeled and controlled to robustly produce monodisperse, nonequilibrium structures strikingly similar to the

  13. ON COMPLEX DYNAMIC CONTROL SYSTEMS

    Institute of Scientific and Technical Information of China (English)

    CHENG Daizhan

    2003-01-01

    This paper presents some recent works on the control of dynamic systems, which have certain complex properties caused by singularity of the nonlinear structures, structure-varyings, or evolution process etc. First, we consider the structure singularity of nonlinear control systems. It was revealed that the focus of researches on nonlinear control theory is shifting from regular systems to singular systems. The singularity of nonlinear systems causes certain complexity. Secondly, the switched systems are considered. For such systems the complexity is caused by the structure varying. We show that the switched systems have significant characteristics of complex systems. Finally, we investigate the evolution systems. The evolution structure makes complexity, and itself is a proper model for complex systems.

  14. Power system dynamics and control

    CERN Document Server

    Kwatny, Harry G

    2016-01-01

    This monograph explores a consistent modeling and analytic framework that provides the tools for an improved understanding of the behavior and the building of efficient models of power systems. It covers the essential concepts for the study of static and dynamic network stability, reviews the structure and design of basic voltage and load-frequency regulators, and offers an introduction to power system optimal control with reliability constraints. A set of Mathematica tutorial notebooks providing detailed solutions of the examples worked-out in the text, as well as a package that will enable readers to work out their own examples and problems, supplements the text. A key premise of the book is that the design of successful control systems requires a deep understanding of the processes to be controlled; as such, the technical discussion begins with a concise review of the physical foundations of electricity and magnetism. This is followed by an overview of nonlinear circuits that include resistors, inductors, ...

  15. Dynamics and Control of a Maglev Vehicle

    Directory of Open Access Journals (Sweden)

    Won ko

    2006-06-01

    Full Text Available In this paper, dynamics of a Maglev vehicle was analyzed and controls utilizing an optimized damping and an LQR algorithms were designed to stabilize the vehicle. The dynamics of magnetically levitated and propelled Maglev vehicle are complex and inherently unstable. Moreover, 6-DOF system dynamics is highly nonlinear and coupled. The proposed control schemes provide the dynamic stability and controllability, which computer simulations confirmed the effectiveness.

  16. On dynamic decoupling and dynamic path controllability in economic systems

    NARCIS (Netherlands)

    Nijmeijer, Henk

    1989-01-01

    In this paper the dynamic decouplability and dynamic path controllability of nonlinear discrete-time economic systems in state space form are discussed. Based on the observation that both properties are equivalent, a (theoretical) efficient way of target path controllability is proposed. This is ill

  17. Simulating Flexible-Spacecraft Dynamics and Control

    Science.gov (United States)

    Fedor, Joseph

    1987-01-01

    Versatile program applies to many types of spacecraft and dynamical problems. Flexible Spacecraft Dynamics and Control program (FSD) developed to aid in simulation of large class of flexible and rigid spacecraft. Extremely versatile and used in attitude dynamics and control analysis as well as in-orbit support of deployment and control of spacecraft. Applicable to inertially oriented spinning, Earth-oriented, or gravity-gradient-stabilized spacecraft. Written in FORTRAN 77.

  18. Control of high affinity interactions in the talin C terminus: how talin domains coordinate protein dynamics in cell adhesions.

    OpenAIRE

    2009-01-01

    In cell-extracellular matrix junctions (focal adhesions), the cytoskeletal protein talin is central to the connection of integrins to the actin cytoskeleton. Talin is thought to mediate this connection via its two integrin, (at least) three actin, and several vinculin binding sites. The binding sites are cryptic in the head-to-rod autoinhibited cytoplasmic form of the protein and require (stepwise) conformational activation. This activation process, however, remains poorly understood, and the...

  19. Multibody system dynamics, robotics and control

    CERN Document Server

    Gerstmayr, Johannes

    2013-01-01

    The volume contains 19 contributions by international experts in the field of multibody system dynamics, robotics and control. The book aims to bridge the gap between the modeling of mechanical systems by means of multibody dynamics formulations and robotics. In the classical approach, a multibody dynamics model contains a very high level of detail, however, the application of such models to robotics or control is usually limited. The papers aim to connect the different scientific communities in multibody dynamics, robotics and control. Main topics are flexible multibody systems, humanoid robots, elastic robots, nonlinear control, optimal path planning, and identification.

  20. Dynamic Programming Method for Impulsive Control Problems

    Science.gov (United States)

    Balkew, Teshome Mogessie

    2015-01-01

    In many control systems changes in the dynamics occur unexpectedly or are applied by a controller as needed. The time at which a controller implements changes is not necessarily known a priori. For example, many manufacturing systems and flight operations have complicated control systems, and changes in the control systems may be automatically…

  1. Dynamic optimization and adaptive controller design

    Science.gov (United States)

    Inamdar, S. R.

    2010-10-01

    In this work I present a new type of controller which is an adaptive tracking controller which employs dynamic optimization for optimizing current value of controller action for the temperature control of nonisothermal continuously stirred tank reactor (CSTR). We begin with a two-state model of nonisothermal CSTR which are mass and heat balance equations and then add cooling system dynamics to eliminate input multiplicity. The initial design value is obtained using local stability of steady states where approach temperature for cooling action is specified as a steady state and a design specification. Later we make a correction in the dynamics where material balance is manipulated to use feed concentration as a system parameter as an adaptive control measure in order to avoid actuator saturation for the main control loop. The analysis leading to design of dynamic optimization based parameter adaptive controller is presented. The important component of this mathematical framework is reference trajectory generation to form an adaptive control measure.

  2. Nonlinear-dynamical arrhythmia control in humans.

    Science.gov (United States)

    Christini, D J; Stein, K M; Markowitz, S M; Mittal, S; Slotwiner, D J; Scheiner, M A; Iwai, S; Lerman, B B

    2001-05-08

    Nonlinear-dynamical control techniques, also known as chaos control, have been used with great success to control a wide range of physical systems. Such techniques have been used to control the behavior of in vitro excitable biological tissue, suggesting their potential for clinical utility. However, the feasibility of using such techniques to control physiological processes has not been demonstrated in humans. Here we show that nonlinear-dynamical control can modulate human cardiac electrophysiological dynamics by rapidly stabilizing an unstable target rhythm. Specifically, in 52/54 control attempts in five patients, we successfully terminated pacing-induced period-2 atrioventricular-nodal conduction alternans by stabilizing the underlying unstable steady-state conduction. This proof-of-concept demonstration shows that nonlinear-dynamical control techniques are clinically feasible and provides a foundation for developing such techniques for more complex forms of clinical arrhythmia.

  3. Controlled interfacial electron dynamics in highly efficient Zn2 SnO4 -based dye-sensitized solar cells.

    Science.gov (United States)

    Shin, Seong Sik; Kim, Dong Wook; Hwang, Daesub; Suk, Jae Ho; Oh, Lee Seul; Han, Byung Suh; Kim, Dong Hoe; Kim, Ju Seong; Kim, Dongho; Kim, Jin Young; Hong, Kug Sun

    2014-02-01

    Among ternary oxides, Zn2 SnO4 (ZSO) is considered for dye-sensitized solar cells (DSSCs) because of its wide bandgap, high optical transmittance, and high electrical conductivity. However, ZSO-based DSSCs have a poor performance record owing largely to the absence of systematic efforts to enhance their performance. Herein, general strategies are proposed to improve the performance of ZSO-based DSSCs involving interfacial engineering/modification of the photoanode. A conformal ZSO thin film (blocking layer) deposited at the fluorine-doped tin oxide-electrolyte interface by pulsed laser deposition suppressed the back-electron transfer effectively while maintaining a high optical transmittance, which resulted in a 22 % improvement in the short-circuit photocurrent density. Surface modification of ZSO nanoparticles (NPs) resulted in an ultrathin ZnO shell layer, a 9 % improvement in the open-circuit voltage, and a 4 % improvement in the fill factor because of the reduced electron recombination at the ZSO NPs-electrolyte interface. The ZSO-based DSSCs exhibited a faster charge injection and electron transport than their TiO2 -based counterparts, and their superior properties were not inhibited by the ZnO shell layer, which indicates their feasibility for highly efficient DSSCs. Each interfacial engineering strategy could be applied to the ZSO-based DSSC independently to lead to an improved conversion efficiency of 6 %, a very high conversion efficiency for a non-TiO2 based DSSC.

  4. Flight Dynamics and Controls Discipline Overview

    Science.gov (United States)

    Theodore, Colin R.

    2012-01-01

    This presentation will touch topics, including but not limited to, the objectives and challenges of flight dynamics and controls that deal with the pilot and the cockpit's technology, the flight dynamics and controls discipline tasks, and the full envelope of flight dynamics modeling. In addition, the LCTR 7x10-ft wind tunnel test will also be included along with the optimal trajectories for noise abatement and its investigations on handling quality. Furthermore, previous experiments and their complying results will also be discussed.

  5. Robot Arm Control Exploiting Natural Dynamics

    Science.gov (United States)

    1999-06-01

    and the approach taken in this thesis is the role of the robot dynamics . In traditional control, the robot is viewed as a general purpose manipulator...robot, and the robot control enforces that command. The robot dynamics are generally ignored or canceled, and certainly do not play a part in how the...task is planned. The approach taken in this thesis is the opposite: the robot dynamics are crucial for the performance of the task as they determine

  6. Neural dynamics for mobile robot adaptive control

    OpenAIRE

    Oubbati, Mohamed

    2006-01-01

    In this thesis, we investigate how dynamics in recurrent neural networks can be used to solve some specific mobile robot problems. We have designed a motion control approach based on a novel recurrent neural network. The advantage of this approach is that, no knowledge about the dynamic model is required, and no synaptic weight changing is needed in presence of time varying parameters. Furthermore, this approach allows a single fixed-weight network to act as a dynamic controller for several d...

  7. Shape dynamics of growing cell walls

    CERN Document Server

    Banerjee, Shiladitya; Dinner, Aaron R

    2015-01-01

    We introduce a general theoretical framework to study the shape dynamics of actively growing and remodeling surfaces. Using this framework we develop a physical model for growing bacterial cell walls and study the interplay of cell shape with the dynamics of growth and constriction. The model allows us to derive constraints on cell wall mechanical energy based on the observed dynamics of cell shape. We predict that exponential growth in cell size requires a constant amount of cell wall energy to be dissipated per unit volume. We use the model to understand and contrast growth in bacteria with different shapes such as spherical, ellipsoidal, cylindrical and toroidal morphologies. Coupling growth to cell wall constriction, we predict a discontinuous shape transformation, from partial constriction to cell division, as a function of the chemical potential driving cell-wall synthesis. Our model for cell wall energy and shape dynamics relates growth kinetics with cell geometry, and provides a unified framework to d...

  8. Dynamic Algorithm for LQGPC Predictive Control

    DEFF Research Database (Denmark)

    Hangstrup, M.; Ordys, A.W.; Grimble, M.J.

    1998-01-01

    In this paper the optimal control law is derived for a multi-variable state space Linear Quadratic Gaussian Predictive Controller (LQGPC). A dynamic performance index is utilized resulting in an optimal steady state controller. Knowledge of future reference values is incorporated into the control...

  9. 质子交换膜燃料电池系统电气输出的动态控制%Dynamic Control of Electric Output Characteristics of Proton Exchange Membrane Fuel Cell System

    Institute of Scientific and Technical Information of China (English)

    刘呈则; 朱新坚

    2005-01-01

    This paper discusses dynamic characteristics of proton exchange membrane fuel cell (PEMFC) under rapid fluctuation of power demand. Wavelet neural network is adopted in the identification of the characteristic curve to predict the voltage. The system control scheme of the voltage and power is introduced. The corresponding schemes for voltage and power control are studied. MATLAB is used to simulate the control system. The results reveal that the adopted control schemes can produce expected effects. Corresponding anti-disturbance and robustness simulation are also carried out. The simulation results show that the implemented control schemes have better robustness and adaptability.

  10. Dynamic Control of Posture Across Locomotor Tasks

    OpenAIRE

    Earhart, Gammon M.

    2013-01-01

    Successful locomotion depends on postural control to establish and maintain appropriate postural orientation of body segments relative to one another and to the environment, and to ensure dynamic stability of the moving body. This paper provides a framework for considering dynamic postural control, highlighting the importance of coordination, consistency, and challenges to postural control posed by various locomotor tasks such as turning and backward walking. The impacts of aging and various ...

  11. Hybrid Predictive Control for Dynamic Transport Problems

    CERN Document Server

    Núñez, Alfredo A; Cortés, Cristián E

    2013-01-01

    Hybrid Predictive Control for Dynamic Transport Problems develops methods for the design of predictive control strategies for nonlinear-dynamic hybrid discrete-/continuous-variable systems. The methodology is designed for real-time applications, particularly the study of dynamic transport systems. Operational and service policies are considered, as well as cost reduction. The control structure is based on a sound definition of the key variables and their evolution. A flexible objective function able to capture the predictive behaviour of the system variables is described. Coupled with efficient algorithms, mainly drawn from the area of computational intelligence, this is shown to optimize performance indices for real-time applications. The framework of the proposed predictive control methodology is generic and, being able to solve nonlinear mixed-integer optimization problems dynamically, is readily extendable to other industrial processes. The main topics of this book are: ●hybrid predictive control (HPC) ...

  12. Controlling edge dynamics in complex networks

    CERN Document Server

    Nepusz, Tamás

    2011-01-01

    The interaction of distinct units in physical, social, biological and technological systems naturally gives rise to complex network structures. Networks have constantly been in the focus of research for the last decade, with considerable advances in the description of their structural and dynamical properties. However, much less effort has been devoted to studying the controllability of the dynamics taking place on them. Here we introduce and evaluate a dynamical process defined on the edges of a network, and demonstrate that the controllability properties of this process significantly differ from simple nodal dynamics. Evaluation of real-world networks indicates that most of them are more controllable than their randomized counterparts. We also find that transcriptional regulatory networks are particularly easy to control. Analytic calculations show that networks with scale-free degree distributions have better controllability properties than uncorrelated networks, and positively correlated in- and out-degre...

  13. Dynamics and control of technical systems

    CERN Document Server

    Balthazar, José M; Kaczmarczyk, Stefan

    2014-01-01

    The main topics of this Special Issue are linear and, mainly, nonlinear dynamics, chaos and control of systems and structures and their applications in different field of science and engineering. According to the goal of the Special Issue, the selected contributions are divided into three major parts: ""Vibration Problems in Vertical Transportation Systems"", ""Nonlinear Dynamics, Chaos and Control of Elastic Structures"" and ""New Strategies and Challenges for Aerospace and Ocean Structures Dynamics and Control"". The discussion of real problems in aerospace and how these problems can be unde

  14. A noise-controlled dynamic bifurcation

    CERN Document Server

    Lythe, G D

    1997-01-01

    We consider a slow passage through a point of loss of stability. If the passage is sufficiently slow, the dynamics are controlled by additive random disturbances, even if they are extremely small. We derive expressions for the `exit value' distribution when the parameter is explicitly a function of time and the dynamics are controlled by additive Gaussian noise. We derive a new expression for the small correction introduced if the noise is coloured (exponentially correlated). There is good agreement with results obtained from simulation of sample paths of the appropriate stochastic differential equations. Multiplicative noise does not produce noise-controlled dynamics in this fashion.

  15. Dynamic control of posture across locomotor tasks.

    Science.gov (United States)

    Earhart, Gammon M

    2013-09-15

    Successful locomotion depends on postural control to establish and maintain appropriate postural orientation of body segments relative to one another and to the environment and to ensure dynamic stability of the moving body. This article provides a framework for considering dynamic postural control, highlighting the importance of coordination, consistency, and challenges to postural control posed by various locomotor tasks, such as turning and backward walking. The impacts of aging and various movement disorders on postural control are discussed broadly in an effort to provide a general overview of the field and recommendations for assessment of dynamic postural control across different populations in both clinical and research settings. Suggestions for future research on dynamic postural control during locomotion also are provided and include discussion of opportunities afforded by new and developing technologies, the need for long-term monitoring of locomotor performance in everyday activities, gaps in our knowledge of how targeted intervention approaches modify dynamic postural control, and the relative paucity of literature regarding dynamic postural control in movement disorder populations other than Parkinson's disease.

  16. Robust control synthesis for uncertain dynamical systems

    Science.gov (United States)

    Byun, Kuk-Whan; Wie, Bong; Sunkel, John

    1989-01-01

    This paper presents robust control synthesis techniques for uncertain dynamical systems subject to structured parameter perturbation. Both QFT (quantitative feedback theory) and H-infinity control synthesis techniques are investigated. Although most H-infinity-related control techniques are not concerned with the structured parameter perturbation, a new way of incorporating the parameter uncertainty in the robust H-infinity control design is presented. A generic model of uncertain dynamical systems is used to illustrate the design methodologies investigated in this paper. It is shown that, for a certain noncolocated structural control problem, use of both techniques results in nonminimum phase compensation.

  17. Controlling edge dynamics in complex networks

    OpenAIRE

    Nepusz, Tamás; Vicsek, Tamás

    2012-01-01

    The interaction of distinct units in physical, social, biological and technological systems naturally gives rise to complex network structures. Networks have constantly been in the focus of research for the last decade, with considerable advances in the description of their structural and dynamical properties. However, much less effort has been devoted to studying the controllability of the dynamics taking place on them. Here we introduce and evaluate a dynamical process defined on the edges ...

  18. Flexible access control for dynamic collaborative environments

    NARCIS (Netherlands)

    Dekker, Mari Antonius Cornelis

    2009-01-01

    Access control is used in computer systems to control access to confidential data. In this thesis we focus on access control for dynamic collaborative environments where multiple users and systems access and exchange data in an ad hoc manner. In such environments it is difficult to protect confident

  19. State estimation for integrated vehicle dynamics control

    NARCIS (Netherlands)

    Zuurbier, J.; Bremmer, P.

    2002-01-01

    This paper discusses a vehicle controller and a state estimator that was implemented and tested in a vehicle equipped with a combined braking and chassis control system to improve handling. The vehicle dynamics controller consists of a feed forward body roll compensation and a feedback stability con

  20. Control of robot dynamics using acceleration control

    Science.gov (United States)

    Workman, G. L.; Prateru, S.; Li, W.; Hinman, Elaine

    1992-01-01

    Acceleration control of robotic devices can provide improvements to many space-based operations using flexible manipulators and to ground-based operations requiring better precision and efficiency than current industrial robots can provide. This paper reports on a preliminary study of acceleration measurement on robotic motion during parabolic flights on the NASA KC-135 and a parallel study of accelerations with and without gravity arising from computer simulated motions using TREETOPS software.

  1. Solved problems in dynamical systems and control

    CERN Document Server

    Tenreiro-Machado, J; Valério, Duarte; Galhano, Alexandra M

    2016-01-01

    This book presents a collection of exercises on dynamical systems, modelling and control. Each topic covered includes a summary of the theoretical background, problems with solutions, and further exercises.

  2. Dynamics and Controls in Maglev Systems

    Science.gov (United States)

    1992-09-01

    and Alscher, H. 1986. "The Magnetic Train Transrapid 06," Proc. Int. Conf. Maglev and Linear Drives, May 14-16, 1986, Vancouver, B.C., Canada, Publ. by...AD-A263 087 ANL-92/43It Il~l Iif IIt[11 Materials and Components Dynamics and Controls Technology Division Materials and Components in Maglev ...Argonne, Illinois 60439 Distribution Category: All Transportation Systems Reports (UC-330) Dynamics and Controls in Maglev Systems by Y. Cai and S. S

  3. Dynamic modeling, simulation and control of energy generation

    CERN Document Server

    Vepa, Ranjan

    2013-01-01

    This book addresses the core issues involved in the dynamic modeling, simulation and control of a selection of energy systems such as gas turbines, wind turbines, fuel cells and batteries. The principles of modeling and control could be applied to other non-convention methods of energy generation such as solar energy and wave energy.A central feature of Dynamic Modeling, Simulation and Control of Energy Generation is that it brings together diverse topics in thermodynamics, fluid mechanics, heat transfer, electro-chemistry, electrical networks and electrical machines and focuses on their appli

  4. Dynamic 3D cell culture via a chemoselective photoactuated ligand.

    Science.gov (United States)

    Westcott, Nathan P; Luo, Wei; Goldstein, Jeffrey; Yousaf, Muhammad N

    2014-09-01

    A new strategy to create a dynamic scaffold for three-dimensional (3D) cell experiments based on a photo-activated cell adhesive peptide ligand is described. After polymerization, the inert matrix becomes cell adhesive by chemoselective modification through the conjugation of oxyamine-terminated ligands. Furthermore, spatial and temporal control of cell culture within the 3D matrix was achieved by the use of a biospecific photoprotected peptide and visualized by confocal microscopy.

  5. About supramolecular systems for dynamically probing cells

    NARCIS (Netherlands)

    Brinkmann, J.; Cavatorta, E.; Sankaran, S.; Schmidt, B.; van Weerd, Jasper; Jonkheijm, Pascal

    2014-01-01

    This article reviews the state of the art in the development of strategies for generating supramolecular systems for dynamic cell studies. Dynamic systems are crucial to further our understanding of cell biology and are consequently at the heart of many medical applications. Increasing interest has

  6. Resilience and Controllability of Dynamic Collective Behaviors

    CERN Document Server

    Komareji, Mohammad

    2014-01-01

    The network paradigm is used to gain insight into the structural root causes of the resilience of consensus in dynamic collective behaviors, and to analyze the controllability of the swarm dynamics. Here we devise the dynamic signaling network which is the information transfer channel underpinning the swarm dynamics of the directed interagent connectivity based on a topological neighborhood of interactions. The study of the connectedness of the swarm signaling network reveals the profound relationship between group size and number of interacting neighbors, which is found to be in good agreement with field observations on flock of starlings [Ballerini et al. (2008) Proc. Natl. Acad. Sci. USA, 105: 1232]. Using a dynamical model, we generate dynamic collective behaviors enabling us to uncover that the swarm signaling network is a homogeneous clustered small-world network, thus facilitating emergent outcomes if connectedness is maintained. Resilience of the emergent consensus is tested by introducing exogenous e...

  7. Discrete Control Processes, Dynamic Games and Multicriterion Control Problems

    Directory of Open Access Journals (Sweden)

    Dumitru Lozovanu

    2002-07-01

    Full Text Available The discrete control processes with state evaluation in time of dynamical system is considered. A general model of control problems with integral-time cost criterion by a trajectory is studied and a general scheme for solving such classes of problems is proposed. In addition the game-theoretical and multicriterion models for control problems are formulated and studied.

  8. Resilience and controllability of dynamic collective behaviors.

    Science.gov (United States)

    Komareji, Mohammad; Bouffanais, Roland

    2013-01-01

    The network paradigm is used to gain insight into the structural root causes of the resilience of consensus in dynamic collective behaviors, and to analyze the controllability of the swarm dynamics. Here we devise the dynamic signaling network which is the information transfer channel underpinning the swarm dynamics of the directed interagent connectivity based on a topological neighborhood of interactions. The study of the connectedness of the swarm signaling network reveals the profound relationship between group size and number of interacting neighbors, which is found to be in good agreement with field observations on flock of starlings [Ballerini et al. (2008) Proc. Natl. Acad. Sci. USA, 105: 1232]. Using a dynamical model, we generate dynamic collective behaviors enabling us to uncover that the swarm signaling network is a homogeneous clustered small-world network, thus facilitating emergent outcomes if connectedness is maintained. Resilience of the emergent consensus is tested by introducing exogenous environmental noise, which ultimately stresses how deeply intertwined are the swarm dynamics in the physical and network spaces. The availability of the signaling network allows us to analytically establish for the first time the number of driver agents necessary to fully control the swarm dynamics.

  9. Dynamic control of chaotic resonators

    KAUST Repository

    Di Falco, A.

    2016-02-16

    We report on the all-optical control of chaotic optical resonators based on silicon on insulator (SOI) platform. We show that simple non-chaotic cavities can be tuned to exhibit chaotic behavior via intense optical pump- ing, inducing a local change of refractive index. To this extent we have fabricated a number of devices and demonstrated experimentally and theoretically that chaos can be triggered on demand on an optical chip. © 2016 SPIE.

  10. Intelligent control algorithm for ship dynamic positioning

    Directory of Open Access Journals (Sweden)

    Meng Wang

    2014-12-01

    Full Text Available Ship motion in the sea is a complex nonlinear kinematics. The hydrodynamic coefficients of ship model are very difficult to accurately determine. Establishing accurate mathematical model of ship motion is difficult because of changing random factors in the marine environment. Aiming at seeking a method of control to realize ship positioning, intelligent control algorithms are adopt utilizing operator's experience. Fuzzy controller and the neural network controller are respectively designed. Through simulations and experiments, intelligent control algorithm can deal with the complex nonlinear motion, and has good robustness. The ship dynamic positioning system with neural network control has high positioning accuracy and performance.

  11. Dynamics and Control of DNA Sequence Amplification

    CERN Document Server

    Marimuthu, Karthikeyan

    2014-01-01

    DNA amplification is the process of replication of a specified DNA sequence \\emph{in vitro} through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first-principles biophysical modeling and control theory. Amplification of DNA is formulated as a problem in control theory with optimal solutions that can differ considerably from strategies typically used in practice. Using the Polymerase Chain Reaction (PCR) as an example, sequence-dependent biophysical models for DNA amplification are cast as control systems, wherein the dynamics of the reaction are controlled by a manipulated input variable. Using these control systems, we demonstrate that there exists an optimal temperature cycling strategy for geometric amplification of any DNA sequence and formulate optimal control problems that can be used to derive the optimal tempe...

  12. Cluster-based control of nonlinear dynamics

    CERN Document Server

    Kaiser, Eurika; Spohn, Andreas; Cattafesta, Louis N; Morzynski, Marek

    2016-01-01

    The ability to manipulate and control fluid flows is of great importance in many scientific and engineering applications. Here, a cluster-based control framework is proposed to determine optimal control laws with respect to a cost function for unsteady flows. The proposed methodology frames high-dimensional, nonlinear dynamics into low-dimensional, probabilistic, linear dynamics which considerably simplifies the optimal control problem while preserving nonlinear actuation mechanisms. The data-driven approach builds upon a state space discretization using a clustering algorithm which groups kinematically similar flow states into a low number of clusters. The temporal evolution of the probability distribution on this set of clusters is then described by a Markov model. The Markov model can be used as predictor for the ergodic probability distribution for a particular control law. This probability distribution approximates the long-term behavior of the original system on which basis the optimal control law is de...

  13. Learning Dynamic Control of Body Roll Orientation

    OpenAIRE

    Vimal, Vivekanand Pandey; Lackner, James R.; DiZio, Paul

    2015-01-01

    Our objective was to examine how the control of orientation is learned in a task involving dynamically balancing about an unstable equilibrium point, the gravitational vertical, in the absence of leg reflexes and muscle stiffness. Subjects (n=10) used a joystick to set themselves to the gravitational vertical while seated in a multi-axis rotation system device (MARS) programmed with inverted pendulum dynamics. The MARS is driven by powerful servomotors and can faithfully follow joystick comma...

  14. Dynamic Actin Controls Polarity Induction de novo in Protoplasts

    Institute of Scientific and Technical Information of China (English)

    Beatrix Zaban; Jan Maisch; Peter Nick

    2013-01-01

    Cell polarity and axes are central for plant morphogenesis.To study how polarity and axes are induced de novo,we investigated protoplasts of tobacco Nicotiana tabacum cv.BY-2 expressing fluorescentlytagged cytoskeletal markers.We standardized the system to such a degree that we were able to generate quantitative data on the temporal patterns of regeneration stages.The synthesis of a new cell wall marks the transition to the first stage of regeneration,and proceeds after a long preparatory phase within a few minutes.During this preparatory phase,the nucleus migrates actively,and cytoplasmic strands remodel vigorously.We probed this system for the effect of anti-cytoskeletal compounds,inducible bundling of actin,RGD-peptides,and temperature.Suppression of actin dynamics at an early stage leads to aberrant tripolar cells,whereas suppression of microtubule dynamics produces aberrant sausagelike cells with asymmetric cell walls.We integrated these data into a model,where the microtubular cytoskeleton conveys positional information between the nucleus and the membrane controlling the release or activation of components required for cell wall synthesis.Cell wall formation is followed by the induction of a new cell pole requiring dynamic actin filaments,and the new cell axis is manifested as elongation growth perpendicular to the orientation of the aligned cortical microtubules.

  15. Dynamic actin controls polarity induction de novo in protoplasts.

    Science.gov (United States)

    Zaban, Beatrix; Maisch, Jan; Nick, Peter

    2013-02-01

    Cell polarity and axes are central for plant morphogenesis. To study how polarity and axes are induced de novo, we investigated protoplasts of tobacco Nicotiana tabacum cv. BY-2 expressing fluorescently-tagged cytoskeletal markers. We standardized the system to such a degree that we were able to generate quantitative data on the temporal patterns of regeneration stages. The synthesis of a new cell wall marks the transition to the first stage of regeneration, and proceeds after a long preparatory phase within a few minutes. During this preparatory phase, the nucleus migrates actively, and cytoplasmic strands remodel vigorously. We probed this system for the effect of anti-cytoskeletal compounds, inducible bundling of actin, RGD-peptides, and temperature. Suppression of actin dynamics at an early stage leads to aberrant tripolar cells, whereas suppression of microtubule dynamics produces aberrant sausage-like cells with asymmetric cell walls. We integrated these data into a model, where the microtubular cytoskeleton conveys positional information between the nucleus and the membrane controlling the release or activation of components required for cell wall synthesis. Cell wall formation is followed by the induction of a new cell pole requiring dynamic actin filaments, and the new cell axis is manifested as elongation growth perpendicular to the orientation of the aligned cortical microtubules.

  16. Approaches to Learning to Control Dynamic Uncertainty

    Directory of Open Access Journals (Sweden)

    Magda Osman

    2015-10-01

    Full Text Available In dynamic environments, when faced with a choice of which learning strategy to adopt, do people choose to mostly explore (maximizing their long term gains or exploit (maximizing their short term gains? More to the point, how does this choice of learning strategy influence one’s later ability to control the environment? In the present study, we explore whether people’s self-reported learning strategies and levels of arousal (i.e., surprise, stress correspond to performance measures of controlling a Highly Uncertain or Moderately Uncertain dynamic environment. Generally, self-reports suggest a preference for exploring the environment to begin with. After which, those in the Highly Uncertain environment generally indicated they exploited more than those in the Moderately Uncertain environment; this difference did not impact on performance on later tests of people’s ability to control the dynamic environment. Levels of arousal were also differentially associated with the uncertainty of the environment. Going beyond behavioral data, our model of dynamic decision-making revealed that, in actual fact, there was no difference in exploitation levels between those in the highly uncertain or moderately uncertain environments, but there were differences based on sensitivity to negative reinforcement. We consider the implications of our findings with respect to learning and strategic approaches to controlling dynamic uncertainty.

  17. Dynamic Surface Control and Its Application to Lateral Vehicle Control

    Directory of Open Access Journals (Sweden)

    Bongsob Song

    2014-01-01

    Full Text Available This paper extends the design and analysis methodology of dynamic surface control (DSC in Song and Hedrick, 2011, for a more general class of nonlinear systems. When rotational mechanical systems such as lateral vehicle control and robot control are considered for applications, sinusoidal functions are easily included in the equation of motions. If such a sinusoidal function is used as a forcing term for DSC, the stability analysis faces the difficulty due to highly nonlinear functions resulting from the low-pass filter dynamics. With modification of input variables to the filter dynamics, the burden of mathematical analysis can be reduced and stability conditions in linear matrix inequality form to guarantee the quadratic stability via DSC are derived for the given class of nonlinear systems. Finally, the proposed design and analysis approach are applied to lateral vehicle control for forward automated driving and backward parallel parking at a low speed as well as an illustrative example.

  18. Phosphorylation site dynamics of early T-cell receptor signaling

    DEFF Research Database (Denmark)

    Chylek, Lily A; Akimov, Vyacheslav; Dengjel, Jörn

    2014-01-01

    a systems-level understanding of how these components cooperate to control signaling dynamics, especially during the crucial first seconds of stimulation. Here, we used quantitative proteomics to characterize reshaping of the T-cell phosphoproteome in response to TCR/CD28 co-stimulation, and found...... that diverse dynamic patterns emerge within seconds. We detected phosphorylation dynamics as early as 5 s and observed widespread regulation of key TCR signaling proteins by 30 s. Development of a computational model pointed to the presence of novel regulatory mechanisms controlling phosphorylation of sites...

  19. Dynamic control of the space tethered system

    Science.gov (United States)

    Malashin, A. A.; Smirnov, N. N.; Bryukvina, O. Yu.; Dyakov, P. A.

    2017-02-01

    We discuss the problem of simultaneous dynamical stabilization and suppression of transverse and longitudinal vibrations of the space tethered system deployed along a certain trajectory. The dynamics of the system is described by a system of nonlinear partial differential equations for the longitudinal and transverse waves and we consider a non-classical version of the problem with one moving boundary. We formulate a mathematical model and perform the analytic and numerical analysis of the boundary control problem based on the Lyapunov method. A scheme of the deployment mechanism is suggested. It includes a control torque and transverse displacement of the boundary and ensures stable deployment of the whole system.

  20. Cell Division, Differentiation and Dynamic Clustering

    CERN Document Server

    Kaneko, K; Kaneko, Kunihiko; Yomo, Tetsuya

    1993-01-01

    A novel mechanism for cell differentiation is proposed, based on the dynamic clustering in a globally coupled chaotic system. A simple model with metabolic reaction, active transport of chemicals from media, and cell division is found to show three successive stages with the growth of the number of cells; coherent growth, dynamic clustering, and fixed cell differentiation. At the last stage, disparity in activities, germ line segregation, somatic cell differentiation, and homeochaotic stability against external perturbation are found. Our results, in consistency with the experiments of the preceding paper, imply that cell differentiation can occur without a spatial pattern. From dynamical systems viewpoint, the new concept of ``open chaos" is proposed, as a novel and general scenario for systems with growing numbers of elements, also seen in economics and sociology.A

  1. Mitochondrial dynamics and morphology in beta-cells.

    Science.gov (United States)

    Stiles, Linsey; Shirihai, Orian S

    2012-12-01

    Mitochondrial dynamics contribute to the regulation of mitochondrial shape as well as various mitochondrial functions and quality control. This is of particular interest in the beta-cell because of the key role mitochondria play in the regulation of beta-cell insulin secretion function. Moreover, mitochondrial dysfunction has been suggested to contribute to the development of Type 2 Diabetes. Genetic tools that shift the balance of mitochondrial fusion and fission result in alterations to beta-cell function and viability. Additionally, conditions that induce beta-cell dysfunction, such as exposure to a high nutrient environment, disrupt mitochondrial morphology and dynamics. While it has been shown that mitochondria display a fragmented morphology in islets of diabetic patients and animal models, the mechanism behind this is currently unknown. Here, we review the current literature on mitochondrial morphology and dynamics in the beta-cell as well as some of the unanswered question in this field.

  2. Regulation and controlled synchronization for complex dynamical systems

    NARCIS (Netherlands)

    Huijberts, H.J.C.; Nijmeijer, Henk; Willems, R.M.A.

    2000-01-01

    In this paper we investigate the problem of controlled synchronization as a regulator problem. In controlled synchronization one is given autonomous transmitter dynamics and controlled receiver dynamics. The question is to find a (output) feedback controller that achieves matching between

  3. Universal signal generator for dynamic cell stimulation.

    Science.gov (United States)

    Piehler, Andreas; Ghorashian, Navid; Zhang, Ce; Tay, Savaş

    2017-06-27

    Dynamic cell stimulation is a powerful technique for probing gene networks and for applications in stem cell differentiation, immunomodulation and signaling. We developed a robust and flexible method and associated microfluidic devices to generate a wide-range of precisely formulated dynamic chemical signals to stimulate live cells and measure their dynamic response. This signal generator is capable of digital to analog conversion (DAC) through combinatoric selection of discrete input concentrations, and outperforms existing methods by both achievable resolution, dynamic range and simplicity in design. It requires no calibration, has minimal space requirements and can be easily integrated into microfluidic cell culture devices. The signal generator hardware and software we developed allows to choose the waveform, period and amplitude of chemical input signals and features addition of well-defined chemical noise to study the role of stochasticity in cellular information processing.

  4. Cell-Size Control

    Science.gov (United States)

    Amodeo, Amanda A.; Skotheim, Jan M.

    2015-01-01

    Cells of a given type maintain a characteristic cell size to function efficiently in their ecological or organismal context. They achieve this through the regulation of growth rates or by actively sensing size and coupling this signal to cell division. We focus this review on potential size-sensing mechanisms, including geometric, external cue, and titration mechanisms. Mechanisms that titrate proteins against DNA are of particular interest because they are consistent with the robust correlation of DNA content and cell size. We review the literature, which suggests that titration mechanisms may underlie cell-size sensing in Xenopus embryos, budding yeast, and Escherichia coli, whereas alternative mechanisms may function in fission yeast. PMID:26254313

  5. Control theory of digitally networked dynamic systems

    CERN Document Server

    Lunze, Jan

    2013-01-01

    The book gives an introduction to networked control systems and describes new modeling paradigms, analysis methods for event-driven, digitally networked systems, and design methods for distributed estimation and control. Networked model predictive control is developed as a means to tolerate time delays and packet loss brought about by the communication network. In event-based control the traditional periodic sampling is replaced by state-dependent triggering schemes. Novel methods for multi-agent systems ensure complete or clustered synchrony of agents with identical or with individual dynamic

  6. Micromechanical Devices for Control of Cell-Cell Interaction, and Methods of Use Thereof

    Science.gov (United States)

    Bhatia, Sangeeta N. (Inventor); Hui, Elliot (Inventor)

    2017-01-01

    The development and function of living tissues depends largely on interactions between cells that can vary in both time and space; however, temporal control of cell-cell interaction is experimentally challenging. By employing a micromachined silicon substrate with moving parts, herein is disclosed the dynamic regulation of cell-cell interactions via direct manipulation of adherent cells with micron-scale precision. The inventive devices and methods allow mechanical control of both tissue composition and spatial organization. The inventive device and methods enable the investigation of dynamic cell-cell interaction in a multitude of applications, such as intercellular communication, spanning embryogenesis, homeostasis, and pathogenic processes.

  7. Controlling Complex Systems and Developing Dynamic Technology

    Science.gov (United States)

    Avizienis, Audrius Victor

    In complex systems, control and understanding become intertwined. Following Ilya Prigogine, we define complex systems as having control parameters which mediate transitions between distinct modes of dynamical behavior. From this perspective, determining the nature of control parameters and demonstrating the associated dynamical phase transitions are practically equivalent and fundamental to engaging with complexity. In the first part of this work, a control parameter is determined for a non-equilibrium electrochemical system by studying a transition in the morphology of structures produced by an electroless deposition reaction. Specifically, changing the size of copper posts used as the substrate for growing metallic silver structures by the reduction of Ag+ from solution under diffusion-limited reaction conditions causes a dynamical phase transition in the crystal growth process. For Cu posts with edge lengths on the order of one micron, local forces promoting anisotropic growth predominate, and the reaction produces interconnected networks of Ag nanowires. As the post size is increased above 10 microns, the local interfacial growth reaction dynamics couple with the macroscopic diffusion field, leading to spatially propagating instabilities in the electrochemical potential which induce periodic branching during crystal growth, producing dendritic deposits. This result is interesting both as an example of control and understanding in a complex system, and as a useful combination of top-down lithography with bottom-up electrochemical self-assembly. The second part of this work focuses on the technological development of devices fabricated using this non-equilibrium electrochemical process, towards a goal of integrating a complex network as a dynamic functional component in a neuromorphic computing device. Self-assembled networks of silver nanowires were reacted with sulfur to produce interfacial "atomic switches": silver-silver sulfide junctions, which exhibit

  8. Chaotic dynamics of controlled electric power systems

    Science.gov (United States)

    Kozlov, V. N.; Trosko, I. U.

    2016-12-01

    The conditions for appearance of chaotic dynamics of electromagnetic and electromechanical processes in energy systems described by the Park-Gorev bilinear differential equations with account for lags of coordinates and restrictions on control have been formulated. On the basis of classical equations, the parameters of synchronous generators and power lines, at which the chaotic dynamics of energy systems appears, have been found. The qualitative and quantitative characteristics of chaotic processes in energy associations of two types, based on the Hopf theorem, and methods of nonstationary linearization and decompositions are given. The properties of spectral characteristics of chaotic processes have been investigated, and the qualitative similarity of bilinear equations of power systems and Lorentz equations have been found. These results can be used for modernization of the systems of control of energy objects. The qualitative and quantitative characteristics for power energy systems as objects of control and for some laws of control with the feedback have been established.

  9. Design and control of swarm dynamics

    CERN Document Server

    Bouffanais, Roland

    2016-01-01

    The book is about the key elements required for designing, building and controlling effective artificial swarms comprised of multiple moving physical agents. Therefore this book presents the fundamentals of each of those key elements in the particular frame of dynamic swarming, specifically exposing the profound connections between these elements and establish some general design principles for swarming behaviors. This scientific endeavor requires an inter-disciplinary approach: biomimetic inspiration from ethology and ecology, study of social information flow, analysis of temporal and adaptive signaling network of interaction, considerations of control of networked real-time systems, and lastly, elements of complex adaptive dynamical systems. This book offers a completely new perspective on the scientific understanding of dynamic collective behaviors thanks to its multi-disciplinary approach and its focus on artificial swarm of physical agents. Two of the key problems in understanding the emergence of swarm ...

  10. Dynamic Control of Kinematically Redundant Robotic Manipulators

    Directory of Open Access Journals (Sweden)

    Erling Lunde

    1987-07-01

    Full Text Available Several methods for task space control of kinematically redundant manipulators have been proposed in the literature. Most of these methods are based on a kinematic analysis of the manipulator. In this paper we propose a control algorithm in which we are especially concerned with the manipulator dynamics. The algorithm is particularly well suited for the class of redundant manipulators consisting of a relatively small manipulator mounted on a larger positioning part.

  11. Power system dynamics stability and control

    CERN Document Server

    Padiyar, K R

    2008-01-01

    Modern power systems tend to be very Complex not only due to increasing Demand for quality power, but also on Account of extensive interconnections and increasing dependence on control for optimum utilization for existing resources. A good Knowledge of system dynamics and control is Essential for secure operation of the system. This book is intended to serve the needs of the Student and practicing engineers. A Large number of illustrative examples are included to provide an insight into the application of the theory.

  12. Controlling Cell Function with Geometry

    Science.gov (United States)

    Mrksich, Milan

    2012-02-01

    This presentation will describe the use of patterned substrates to control cell shape with examples that illustrate the ways in which cell shape can regulate cell function. Most cells are adherent and must attach to and spread on a surface in order to survive, proliferate and function. In tissue, this surface is the extracellular matrix (ECM), an insoluble scaffold formed by the assembly of several large proteins---including fibronectin, the laminins and collagens and others---but in the laboratory, the surface is prepared by adsorbing protein to glass slides. To pattern cells, gold-coated slides are patterned with microcontact printing to create geometric features that promote cell attachment and that are surrounded by inert regions. Cells attach to these substrates and spread to adopt the shape defined by the underlying pattern and remain stable in culture for several days. Examples will be described that used a series of shapes to reveal the relationship between the shape of the cell and the structure of its cytoskeleton. These geometric cues were used to control cell polarity and the tension, or contractility, present in the cytoskeleton. These rules were further used to control the shapes of mesenchymal stem cells and in turn to control the differentiation of these cells into specialized cell types. For example, stem cells that were patterned into a ``star'' shape preferentially differentiated into bone cells whereas those that were patterned into a ``flower'' shape preferred a fat cell fate. These influences of shape on differentiation depend on the mechanical properties of the cytoskeleton. These examples, and others, reveal that shape is an important cue that informs cell function and that can be combined with the more common soluble cues to direct and study cell function.

  13. The Matrix exponential, Dynamic Systems and Control

    DEFF Research Database (Denmark)

    Poulsen, Niels Kjølstad

    2004-01-01

    The matrix exponential can be found in various connections in analysis and control of dynamic systems. In this short note we are going to list a few examples. The matrix exponential usably pops up in connection to the sampling process, whatever it is in a deterministic or a stochastic setting...

  14. Controlling Chemistry in Dynamic Nanoscale Systems

    DEFF Research Database (Denmark)

    Jesorka, Aldo; Lizana, Ludvig; Konkoli, Zoran

    2011-01-01

    of the concept. Controlled release of chol-DNA molecules from SU-8 surfaces gives the possibility to dynamically change surface and/or solution properties in micro and nanoreactor applications, opening access to stable 2D chemistry on surface-based devices with potential for easy interfacing with conventional...

  15. The Matrix exponential, Dynamic Systems and Control

    DEFF Research Database (Denmark)

    Poulsen, Niels Kjølstad

    2004-01-01

    The matrix exponential can be found in various connections in analysis and control of dynamic systems. In this short note we are going to list a few examples. The matrix exponential usably pops up in connection to the sampling process, whatever it is in a deterministic or a stochastic setting...

  16. Dynamic culture improves cell reprogramming efficiency.

    Science.gov (United States)

    Sia, Junren; Sun, Raymond; Chu, Julia; Li, Song

    2016-06-01

    Cell reprogramming to pluripotency is an inefficient process and various approaches have been devised to improve the yield of induced pluripotent stem cells. However, the effect of biophysical factors on cell reprogramming is not well understood. Here we showed that, for the first time, dynamic culture with orbital shaking significantly improved the reprogramming efficiency in adherent cells. Manipulating the viscosity of the culture medium suggested that the improved efficiency is mainly attributed to convective mixing rather than hydrodynamic shear stress. Temporal studies demonstrated that the enhancement of reprogramming efficiency required the dynamic culture in the middle but not early phase. In the early phase, fibroblasts had a high proliferation rate, but as the culture became over-confluent in the middle phase, expression of p57 was upregulated to inhibit cell proliferation and consequently, cell reprogramming. Subjecting the over confluent culture to orbital shaking prevented the upregulation of p57, thus improving reprogramming efficiency. Seeding cells at low densities to avoid over-confluency resulted in a lower efficiency, and optimal reprogramming efficiency was attained at a high seeding density with dynamic culture. Our findings provide insight into the underlying mechanisms of how dynamic culture condition regulate cell reprogramming, and will have broad impact on cell engineering for regenerative medicine and disease modeling.

  17. Dynamics and control of DNA sequence amplification

    Energy Technology Data Exchange (ETDEWEB)

    Marimuthu, Karthikeyan [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Chakrabarti, Raj, E-mail: raj@pmc-group.com, E-mail: rajc@andrew.cmu.edu [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Division of Fundamental Research, PMC Advanced Technology, Mount Laurel, New Jersey 08054 (United States)

    2014-10-28

    DNA amplification is the process of replication of a specified DNA sequence in vitro through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first-principles biophysical modeling and control theory. Amplification of DNA is formulated as a problem in control theory with optimal solutions that can differ considerably from strategies typically used in practice. Using the Polymerase Chain Reaction as an example, sequence-dependent biophysical models for DNA amplification are cast as control systems, wherein the dynamics of the reaction are controlled by a manipulated input variable. Using these control systems, we demonstrate that there exists an optimal temperature cycling strategy for geometric amplification of any DNA sequence and formulate optimal control problems that can be used to derive the optimal temperature profile. Strategies for the optimal synthesis of the DNA amplification control trajectory are proposed. Analogous methods can be used to formulate control problems for more advanced amplification objectives corresponding to the design of new types of DNA amplification reactions.

  18. Dynamics and control of underactuated multibody spacecraft

    Science.gov (United States)

    Cho, Sangbum

    In this dissertation, we develop equations of motion for a class of multibody spacecraft consisting of a rigid base body and multiple rigid appendages connected to the base body. There has been much prior research on this topic; however, much of this research is not appropriate for nonlinear control design purposes. The motion of a multibody spacecraft is described by the position and attitude of a base body in an inertial frame and by the relative position and attitude of the attached bodies with respect to the base body; these latter quantities define the shape of the multibody spacecraft. Our aim is to develop equations of motion that reveal important nonlinear coupling effects between the translation, rotation and shape dynamics, but are simple enough for control design purposes. A rotation matrix is used to represent the attitude of the spacecraft. This allows us to avoid complexity related to the use of parameter representations such as Euler angles. Hamilton's variational principle gives three sets of nonlinear equations of motion. The latter part of this dissertation presents results of control problems for several underactuated multibody spacecraft examples. These include spacecraft with an unactuated internal sliding mass, spacecraft with unactuated fuel slosh dynamics, tethered spacecraft with attachment point actuation and the triaxial attitude control testbed with two proof mass actuation devices. These examples illustrate important features related to the dynamics and control of various underactuated multibody spacecraft. Differences in geometries of the spacecraft and gravitational assumptions require adoption of different types of control schemes. We use the multibody equations in this dissertation to formulate control equations for the models and to construct feedback controllers that achieves asymptotic stability (or convergence) to the desired (relative) equilibrium manifolds. Computer simulations demonstrate the effectiveness of the controllers.

  19. Optimal Control of Isometric Muscle Dynamics

    Directory of Open Access Journals (Sweden)

    Robert Rockenfeller

    2015-03-01

    Full Text Available We use an indirect optimal control approach to calculate the optimal neural stimulation needed to obtain measured isometric muscle forces. The neural stimulation of the nerve system is hereby considered to be a control function (input of the system ’muscle’ that solely determines the muscle force (output. We use a well-established muscle model and experimental data of isometric contractions. The model consists of coupled activation and contraction dynamics described by ordinary differential equations. To validate our results, we perform a comparison with commercial optimal control software.

  20. Dynamical Adaptation in Terrorist Cells/Networks

    DEFF Research Database (Denmark)

    Hussain, Dil Muhammad Akbar; Ahmed, Zaki

    2010-01-01

    Typical terrorist cells/networks have dynamical structure as they evolve or adapt to changes which may occur due to capturing or killing of a member of the cell/network. Analytical measures in graph theory like degree centrality, betweenness and closeness centralities are very common and have long...

  1. Sorting cells by their dynamical properties

    Science.gov (United States)

    Henry, Ewan; Holm, Stefan H.; Zhang, Zunmin; Beech, Jason P.; Tegenfeldt, Jonas O.; Fedosov, Dmitry A.; Gompper, Gerhard

    2016-10-01

    Recent advances in cell sorting aim at the development of novel methods that are sensitive to various mechanical properties of cells. Microfluidic technologies have a great potential for cell sorting; however, the design of many micro-devices is based on theories developed for rigid spherical particles with size as a separation parameter. Clearly, most bioparticles are non-spherical and deformable and therefore exhibit a much more intricate behavior in fluid flow than rigid spheres. Here, we demonstrate the use of cells’ mechanical and dynamical properties as biomarkers for separation by employing a combination of mesoscale hydrodynamic simulations and microfluidic experiments. The dynamic behavior of red blood cells (RBCs) within deterministic lateral displacement (DLD) devices is investigated for different device geometries and viscosity contrasts between the intra-cellular fluid and suspending medium. We find that the viscosity contrast and associated cell dynamics clearly determine the RBC trajectory through a DLD device. Simulation results compare well to experiments and provide new insights into the physical mechanisms which govern the sorting of non-spherical and deformable cells in DLD devices. Finally, we discuss the implications of cell dynamics for sorting schemes based on properties other than cell size, such as mechanics and morphology.

  2. Controlling complex Langevin dynamics at finite density

    Energy Technology Data Exchange (ETDEWEB)

    Aarts, Gert; Bongiovanni, Lorenzo [Swansea University, Department of Physics, College of Science, Swansea (United Kingdom); Seiler, Erhard [Max-Planck-Institut fuer Physik (Werner-Heisenberg-Institut), Muenchen (Germany); Sexty, Denes [Universitaet Heidelberg, Institut fuer Theoretische Physik, Heidelberg (Germany); Stamatescu, Ion-Olimpiu [Universitaet Heidelberg, Institut fuer Theoretische Physik, Heidelberg (Germany); FEST, Heidelberg (Germany)

    2013-07-15

    At nonzero chemical potential the numerical sign problem in lattice field theory limits the use of standard algorithms based on importance sampling. Complex Langevin dynamics provides a possible solution, but it has to be applied with care. In this review, we first summarise our current understanding of the approach, combining analytical and numerical insight. In the second part we study SL(N,C) gauge cooling, which was introduced recently as a tool to control complex Langevin dynamics in nonabelian gauge theories. We present new results in Polyakov chain models and in QCD with heavy quarks and compare various adaptive cooling implementations. (orig.)

  3. Dynamics and control strategies for a butanol fermentation process.

    Science.gov (United States)

    Mariano, Adriano Pinto; Costa, Caliane Bastos Borba; Maciel, Maria Regina Wolf; Maugeri Filho, Francisco; Atala, Daniel Ibraim Pires; de Angelis, Dejanira de Franceschi; Maciel Filho, Rubens

    2010-04-01

    In this work, mathematical modeling was employed to assess the dynamic behavior of the flash fermentation process for the production of butanol. This process consists of three interconnected units as follows: fermentor, cell retention system (tangential microfiltration), and vacuum flash vessel (responsible for the continuous recovery of butanol from the broth). Based on the study of the dynamics of the process, suitable feedback control strategies [single input/single output (SISO) and multiple input/multiple output (MIMO)] were elaborated to deal with disturbances related to the process. The regulatory control consisted of keeping sugar and/or butanol concentrations in the fermentor constant in the face of disturbances in the feed substrate concentration. Another objective was the maintenance of the proper operation of the flash tank (maintenance of the thermodynamic equilibrium of the liquid and vapor phases) considering that oscillations in the temperature in the tank are expected. The servo control consisted of changes in concentration set points. The performance of an advanced controller, the dynamic matrix control, and the classical proportional-integral controller was evaluated. Both controllers were able to regulate the operating conditions in order to accommodate the perturbations with the lowest possible alterations in the process outputs. However, the performance of the PI controller was superior because it showed quicker responses without oscillations.

  4. Synthetic multicellular oscillatory systems: controlling protein dynamics with genetic circuits

    Energy Technology Data Exchange (ETDEWEB)

    Koseska, Aneta [Interdisciplinary Center for Dynamics of Complex Systems, University of Potsdam, D-14469 Potsdam (Germany); Volkov, Evgenii [Department of Theoretical Physics, Lebedev Physical Institute, Leninskii 53, Moscow (Russian Federation); Kurths, Juergen, E-mail: akoseska@uni-potsdam.de [Institute of Physics, Humboldt University Berlin, D-10099 Berlin (Germany)

    2011-10-15

    Synthetic biology is a relatively new research discipline that combines standard biology approaches with the constructive nature of engineering. Thus, recent efforts in the field of synthetic biology have given a perspective to consider cells as 'programmable matter'. Here, we address the possibility of using synthetic circuits to control protein dynamics. In particular, we show how intercellular communication and stochasticity can be used to manipulate the dynamical behavior of a population of coupled synthetic units and, in this manner, finely tune the expression of specific proteins of interest, e.g. in large bioreactors.

  5. Success Stories in Control: Nonlinear Dynamic Inversion Control

    Science.gov (United States)

    Bosworth, John T.

    2010-01-01

    NASA plays an important role in advancing the state of the art in flight control systems. In the case of Nonlinear Dynamic Inversion (NDI) NASA supported initial implementation of the theory in an aircraft and demonstration in a space vehicle. Dr. Dale Enns of Honeywell Aerospace Advanced Technology performed this work in cooperation with NASA and under NASA contract. Honeywell and Lockheed Martin were subsequently contracted by AFRL to create "Design Guidelines for Multivariable Control Theory". This foundational work directly contributed to the advancement of the technology and the credibility of the control law as a design option. As a result Honeywell collaborated with Lockheed Martin to produce a Nonlinear Dynamic Inversion controller for the X-35 and subsequently Lockheed Martin did the same for the production Lockheed Martin F-35 vehicle. The theory behind NDI is to use a systematic generalized approach to controlling a vehicle. Using general aircraft nonlinear equations of motion and onboard aerodynamic, mass properties, and engine models specific to the vehicle, a relationship between control effectors and desired aircraft motion can be formulated. Using this formulation a control combination is used that provides a predictable response to commanded motion. Control loops around this formulation shape the response as desired and provide robustness to modeling errors. Once the control law is designed it can be used on a similar class of vehicle with only an update to the vehicle specific onboard models.

  6. Controlling flow time delays in flexible manufacturing cells

    NARCIS (Netherlands)

    Slomp, J.; Caprihan, R.; Bokhorst, J. A. C.

    2009-01-01

    Flow time delays in Flexible Manufacturing Cells (FMCs) are caused by transport and clamping/reclamping activities. This paper shows how dynamic scheduling parameters may control the flow times of jobs and the available task windows for flow time delays.

  7. Sensitivity Analysis of Centralized Dynamic Cell Selection

    DEFF Research Database (Denmark)

    Lopez, Victor Fernandez; Alvarez, Beatriz Soret; Pedersen, Klaus I.;

    2016-01-01

    mechanism and solutions involving cell switching in general. Simulation results show that such solutions can greatly benefit from the use of receivers with interference suppression capabilities and a larger number of antennas, with a maximum data rate gain of 120%. High performance gains are observed...... with two different traffic models, and it is not necessary to be able to connect to a large number of cells in order to reap most of the benefits of the centralized dynamic cell selection....

  8. Dynamic control of metamaterials at terahertz frequencies

    Science.gov (United States)

    Shrekenhamer, David

    Progress in the field of metamaterials has started coming to a point where the field may finally begin to emerge as a viable solution to many electromagnetic challenges facing the community. No where is that more true then at terahertz frequencies where there lies an immense opportunity for growth. The development of mature technologies within this region of the electromagnetic spectrum would provide a valuable resource to become available for a multitude of applications. In order to achieve this, the necessary first steps of identifying viable materials and paths to integrate these with metamaterials will need to be completed. In this dissertation, we examine several different paths to achieve dynamic metamaterial electromagnetic response at terahertz frequencies, and demonstrate several paths to package these devices into imaging systems. In Chapter 1, we introduce the basic theory and design principles of metamaterials. We also describe the experimental techniques involved in the study of terahertz metamaterials. Chapter 2 presents a computational and experimental study investigating the integration of high electron mobility transistors with metamaterials allowing for high speed modulation of incident terahertz radiation. In Chapters 3 and 4, we investigate several different paths to create tunable terahertz metamaterial absorbers. Chapter 3 presents an investigation where we encapsulate a metametarial absorber unit cell with liquid crystals. We study both computationally and experimentally the tuning mechanism of the absorber as the liquid crystal refractive index is controlled as a function of the applied electric field strength and modulation frequency. In Chapter 4, we form a doped semiconducting metamaterial spatial light modulator with multi-color super-pixels composed of arrays of electronically controlled terahertz metamaterial absorbers. We computationally and experimentally study the independent tunability of each pixel in the spatial array and

  9. Hybrid dynamical systems observation and control

    CERN Document Server

    Defoort, Michael

    2015-01-01

    This book is a collection of contributions defining the state of current knowledge and new trends in hybrid systems – systems involving both continuous dynamics and discrete events – as described by the work of several well-known groups of researchers. Hybrid Dynamical Systems presents theoretical advances in such areas as diagnosability, observability and stabilization for various classes of system. Continuous and discrete state estimation and self-triggering control of nonlinear systems are advanced. The text employs various methods, among them, high-order sliding modes, Takagi–Sugeno representation and sampled-data switching to achieve its ends. The many applications of hybrid systems from power converters to computer science are not forgotten; studies of flexible-joint robotic arms and – as representative biological systems – the behaviour of the human heart and vasculature, demonstrate the wide-ranging practical significance of control in hybrid systems. The cross-disciplinary origins of study ...

  10. Numerical Investigations of Dynamic Stall Control

    Directory of Open Access Journals (Sweden)

    Florin FRUNZULICA

    2014-04-01

    Full Text Available In this paper we investigated numerically the dynamic stall phenomenon and the possibilities to control it, with application to vertical axis wind turbines (for urban users. The Phenomenon appear at low tip speed ratio (TSR<4 and it has a great impact on structural integrity of the wind turbine and power performances. For this reason we performed a computational study of dynamic stall around NACA 0012 airfoil in pitching motion at relative low Reynolds number (105. Also, we performed the same analysis for four flow control methods: two passive (Gurney flap and slot and two active (blowing jet on the rounded trailing edge and synthetic jet periodically activated. The Results are compared to those of an existing experimental case test.

  11. Electron dynamics controlled via self-interaction

    CERN Document Server

    Tamburini, Matteo; Di Piazza, Antonino

    2013-01-01

    The dynamics of an electron in a strong laser field can be significantly altered by radiation reaction. This usually results in a strongly damped motion, with the electron losing a large fraction of its initial energy. Here we show that the electron dynamics in a bichromatic laser pulse can be indirectly controlled by a comparatively small radiation reaction force through its interplay with the Lorentz force. By changing the relative phase between the two frequency components of the bichromatic laser field, an ultrarelativistic electron bunch colliding head-on with the laser pulse can be deflected in a controlled way, with the deflection angle being independent of the initial electron energy. The effect is predicted to be observable with intensities available at upcoming laser facilities.

  12. Energy Management Dynamic Control Topology In MANET

    Science.gov (United States)

    Madhusudan, G.; Kumar, TNR

    2017-08-01

    Topology management via per-node transmission power adjustment has been shown effective in extending network lifetime. The existing algorithms constructs static topologies which fail to take the residual energy of network nodes, and cannot balance energy consumption efficiently. To address this problem, a Light Weighted Distributed Topology Control algorithm EMDCT(Energy Management Dynamic Control Topology ) is proposed in this paper. Based on the link metric of the network, both the energy consumption rate level and residual energy levels at the two end nodes are considered. EMDCT generates a Dynamic Topology that changes with the variation of node energy without the aid of location information, each node determines its transmission power according to local network information, which reduces the overhead complexity of EMDCT greatly. The experiment results show that EMDCT preserves network connectivity and manitains minimum-cost property of the network also it can extend network lifetime more remarkably.

  13. Dynamics of Dengue epidemics using optimal control

    CERN Document Server

    Rodrigues, Helena Sofia; Torres, Delfim F M

    2010-01-01

    We present an application of optimal control theory to Dengue epidemics. This epidemiologic disease is an important theme in tropical countries due to the growing number of infected individuals. The dynamic model is described by a set of nonlinear ordinary differential equations, that depend on the dynamic of the Dengue mosquito, the number of infected individuals, and the people's motivation to combat the mosquito. The cost functional depends not only on the costs of medical treatment of the infected people but also on the costs related to educational and sanitary campaigns. Two approaches to solve the problem are considered: one using optimal control theory, another one by discretizing first the problem and then solving it with nonlinear programming. The results obtained with OC-ODE and IPOPT solvers are given and discussed. We observe that with current computational tools it is easy to obtain, in an efficient way, better solutions to Dengue problems, leading to a decrease of infected mosquitoes and individ...

  14. Dynamic Characteristics of Explicit Control Protocol

    Institute of Scientific and Technical Information of China (English)

    CHENG Shuang-mei; LI Jian-hua; GUO Chuan-xiong

    2008-01-01

    The dynamic characteristics of eplicit control protocol (XCP) were investigated with single bottle-neck on the microscopic time-scale. Analysis and simulation results show that the bandwidth utilization of an XCP bottleneck link converges to 1 at exponential rate, persistent congestion cannot occur at the bottleneck link, and throughput of an arbitrary subset of XCP flows at the bottleneck link converges to its fair share in exponential rate. The XCP has high bandwidth utilization and good fairness properties.

  15. Cell Control Engineering

    DEFF Research Database (Denmark)

    Lynggaard, Hans Jørgen Birk; Alting, Leo

    1996-01-01

    strategy. The concept has been validated and verified at Odense Steel Shipyard Ltd. in several robotic arc welding systems in the production using an application enabler, UNIX, X, SQL and (wireless) Ethernet. Integration with off-line programming, production planning, monitoring and maintenance has been...... of any production installation and in addition the software element is often very critical as it is the integrating, controlling and co-ordinating system component. As such, a CCE concept providing high quality and high functionality as well as flexibility is an important aspect of a company's automation...

  16. Neuromechanical tuning of nonlinear postural control dynamics

    Science.gov (United States)

    Ting, Lena H.; van Antwerp, Keith W.; Scrivens, Jevin E.; McKay, J. Lucas; Welch, Torrence D. J.; Bingham, Jeffrey T.; DeWeerth, Stephen P.

    2009-06-01

    Postural control may be an ideal physiological motor task for elucidating general questions about the organization, diversity, flexibility, and variability of biological motor behaviors using nonlinear dynamical analysis techniques. Rather than presenting "problems" to the nervous system, the redundancy of biological systems and variability in their behaviors may actually be exploited to allow for the flexible achievement of multiple and concurrent task-level goals associated with movement. Such variability may reflect the constant "tuning" of neuromechanical elements and their interactions for movement control. The problem faced by researchers is that there is no one-to-one mapping between the task goal and the coordination of the underlying elements. We review recent and ongoing research in postural control with the goal of identifying common mechanisms underlying variability in postural control, coordination of multiple postural strategies, and transitions between them. We present a delayed-feedback model used to characterize the variability observed in muscle coordination patterns during postural responses to perturbation. We emphasize the significance of delays in physiological postural systems, requiring the modulation and coordination of both the instantaneous, "passive" response to perturbations as well as the delayed, "active" responses to perturbations. The challenge for future research lies in understanding the mechanisms and principles underlying neuromechanical tuning of and transitions between the diversity of postural behaviors. Here we describe some of our recent and ongoing studies aimed at understanding variability in postural control using physical robotic systems, human experiments, dimensional analysis, and computational models that could be enhanced from a nonlinear dynamics approach.

  17. Dynamic nonprehensile manipulation: Controllability, planning, and experiments

    Energy Technology Data Exchange (ETDEWEB)

    Lynch, K.M. [Northwestern Univ., Evanston, IL (United States). Dept. of Mechanical Engineering; Mason, M.T. [Carnegie Mellon Univ., Pittsburgh, PA (United States). Robotics Inst.

    1999-01-01

    The authors are interested in using low-degree-of-freedom robots to perform complex tasks by nonprehensile manipulation (manipulation without a form- or force-closure grasp). By not grasping, the robot can use gravitational, centrifugal, and Coriolis forces as virtual motors to control more degrees of freedom of the part. The part`s extra motion freedoms are exhibited as rolling, slipping, and free flight. This paper describes controllability, motion planning, and implementation of planar dynamic nonprehensile manipulation. The authors show that almost any planar object is controllable by point contact, and the controlling robot requires only two degrees of freedom (a point translating in the plane). They then focus on a one-joint manipulator (with a two-dimensional state space), and show that even this simplest of robots, by using slipping and rolling, can control a planar object to a full-dimensional subset of its six-0dimensional state space. The authors have developed a one-joint robot to perform a variety of dynamic tasks, including snatching an object from a table, rolling an object on the surface of the arm, and throwing and catching. Nonlinear optimization is used to plan robot trajectories that achieve the desired object motion via coupling forces through the nonprehensile contact.

  18. Chaos control applied to heart rhythm dynamics

    Energy Technology Data Exchange (ETDEWEB)

    Borem Ferreira, Bianca, E-mail: biaborem@gmail.com [Universidade Federal do Rio de Janeiro, COPPE, Department of Mechanical Engineering, P.O. Box 68.503, 21.941.972 Rio de Janeiro, RJ (Brazil); Souza de Paula, Aline, E-mail: alinedepaula@unb.br [Universidade de Brasi' lia, Department of Mechanical Engineering, 70.910.900 Brasilia, DF (Brazil); Amorim Savi, Marcelo, E-mail: savi@mecanica.ufrj.br [Universidade Federal do Rio de Janeiro, COPPE, Department of Mechanical Engineering, P.O. Box 68.503, 21.941.972 Rio de Janeiro, RJ (Brazil)

    2011-08-15

    Highlights: > A natural cardiac pacemaker is modeled by a modified Van der Pol oscillator. > Responses related to normal and chaotic, pathological functioning of the heart are investigated. > Chaos control methods are applied to avoid pathological behaviors of heart dynamics. > Different approaches are treated: stabilization of unstable periodic orbits and chaos suppression. - Abstract: The dynamics of cardiovascular rhythms have been widely studied due to the key aspects of the heart in the physiology of living beings. Cardiac rhythms can be either periodic or chaotic, being respectively related to normal and pathological physiological functioning. In this regard, chaos control methods may be useful to promote the stabilization of unstable periodic orbits using small perturbations. In this article, the extended time-delayed feedback control method is applied to a natural cardiac pacemaker described by a mathematical model. The model consists of a modified Van der Pol equation that reproduces the behavior of this pacemaker. Results show the ability of the chaos control strategy to control the system response performing either the stabilization of unstable periodic orbits or the suppression of chaotic response, avoiding behaviors associated with critical cardiac pathologies.

  19. Controlling Proton Delivery through Catalyst Structural Dynamics

    Energy Technology Data Exchange (ETDEWEB)

    Cardenas, Allan Jay P. [Center for Molecular Electrocatalysis, Pacific Northwest National Laboratory, P.O. Box 999, K2-57 Richland WA 99352 USA; 221 Science Center, State University of New York at Fredonia, Fredonia NY 14063 USA; Ginovska, Bojana [Center for Molecular Electrocatalysis, Pacific Northwest National Laboratory, P.O. Box 999, K2-57 Richland WA 99352 USA; Kumar, Neeraj [Center for Molecular Electrocatalysis, Pacific Northwest National Laboratory, P.O. Box 999, K2-57 Richland WA 99352 USA; Hou, Jianbo [Center for Molecular Electrocatalysis, Pacific Northwest National Laboratory, P.O. Box 999, K2-57 Richland WA 99352 USA; Raugei, Simone [Center for Molecular Electrocatalysis, Pacific Northwest National Laboratory, P.O. Box 999, K2-57 Richland WA 99352 USA; Helm, Monte L. [Center for Molecular Electrocatalysis, Pacific Northwest National Laboratory, P.O. Box 999, K2-57 Richland WA 99352 USA; Appel, Aaron M. [Center for Molecular Electrocatalysis, Pacific Northwest National Laboratory, P.O. Box 999, K2-57 Richland WA 99352 USA; Bullock, R. Morris [Center for Molecular Electrocatalysis, Pacific Northwest National Laboratory, P.O. Box 999, K2-57 Richland WA 99352 USA; O' Hagan, Molly [Center for Molecular Electrocatalysis, Pacific Northwest National Laboratory, P.O. Box 999, K2-57 Richland WA 99352 USA

    2016-09-27

    The fastest synthetic molecular catalysts for production and oxidation of H2 emulate components of the active site of natural hydrogenases. The role of controlled structural dynamics is recognized as a critical component in the catalytic performance of many enzymes, including hydrogenases, but is largely neglected in the design of synthetic molecular cata-lysts. In this work, the impact of controlling structural dynamics on the rate of production of H2 was studied for a series of [Ni(PPh2NC6H4-R2)2]2+ catalysts including R = n-hexyl, n-decyl, n-tetradecyl, n-octadecyl, phenyl, or cyclohexyl. A strong correlation was observed between the ligand structural dynamics and the rates of electrocatalytic hydrogen production in acetonitrile, acetonitrile-water, and protic ionic liquid-water mixtures. Specifically, the turnover frequencies correlate inversely with the rates of ring inversion of the amine-containing ligand, as this dynamic process dictates the positioning of the proton relay in the second coordination sphere and therefore governs protonation at either catalytically productive or non-productive sites. This study demonstrates that the dynamic processes involved in proton delivery can be controlled through modifications of the outer coordination sphere of the catalyst, similar to the role of the protein architecture in many enzymes. The present work provides new mechanistic insight into the large rate enhancements observed in aqueous protic ionic liquid media for the [Ni(PPh2NR2)]2+ family of catalysts. The incorporation of controlled structural dynamics as a design parameter to modulate proton delivery in molecular catalysts has enabled H2 production rates that are up to three orders of magnitude faster than the [Ni(PPh2NPh2)]2+complex. The observed turnover frequencies are up to 106 s-1 in acetonitrile-water, and over 107 s-1 in protic ionic liquid-water mixtures, with a minimal increase in overpotential. This material is based upon work supported as part of

  20. Simulation, optimal control and parametric sensitivity analysis of a molten carbonate fuel cell using a partial differential algebraic dynamic equation system; Simulation, Optimale Steuerung und Sensitivitaetsanalyse einer Schmelzkarbonat-Brennstoffzelle mithilfe eines partiellen differential-algebraischen dynamischen Gleichungssystems

    Energy Technology Data Exchange (ETDEWEB)

    Sternberg, K.

    2007-02-08

    Molten carbonate fuel cells (MCFCs) allow an efficient and environmentally friendly energy production by converting the chemical energy contained in the fuel gas in virtue of electro-chemical reactions. In order to predict the effect of the electro-chemical reactions and to control the dynamical behavior of the fuel cell a mathematical model has to be found. The molten carbonate fuel cell (MCFC) can indeed be described by a highly complex,large scale, semi-linear system of partial differential algebraic equations. This system includes a reaction-diffusion-equation of parabolic type, several reaction-transport-equations of hyperbolic type, several ordinary differential equations and finally a system of integro-differential algebraic equations which describes the nonlinear non-standard boundary conditions for the entire partial differential algebraic equation system (PDAE-system). The existence of an analytical or the computability of a numerical solution for this high-dimensional PDAE-system depends on the kind of the differential equations and their special characteristics. Apart from theoretical investigations, the real process has to be controlled, more precisely optimally controlled. Hence, on the basis of the PDAE-system an optimal control problem is set up, whose analytical and numerical solvability is closely linked to the solvability of the PDAE-system. Moreover the solution of that optimal control problem is made more difficult by inaccuracies in the underlying database, which does not supply sufficiently accurate values for the model parameters. Therefore the optimal control problem must also be investigated with respect to small disturbances of model parameters. The aim of this work is to analyze the relevant dynamic behavior of MCFCs and to develop concepts for their optimal process control. Therefore this work is concerned with the simulation, the optimal control and the sensitivity analysis of a mathematical model for MCDCs, which can be characterized

  1. Adaptive Dynamic Surface Control for Generator Excitation Control System

    Directory of Open Access Journals (Sweden)

    Zhang Xiu-yu

    2014-01-01

    Full Text Available For the generator excitation control system which is equipped with static var compensator (SVC and unknown parameters, a novel adaptive dynamic surface control scheme is proposed based on neural network and tracking error transformed function with the following features: (1 the transformation of the excitation generator model to the linear systems is omitted; (2 the prespecified performance of the tracking error can be guaranteed by combining with the tracking error transformed function; (3 the computational burden is greatly reduced by estimating the norm of the weighted vector of neural network instead of the weighted vector itself; therefore, it is more suitable for the real time control; and (4 the explosion of complicity problem inherent in the backstepping control can be eliminated. It is proved that the new scheme can make the system semiglobally uniformly ultimately bounded. Simulation results show the effectiveness of this control scheme.

  2. Wake flow control using a dynamically controlled wind turbine

    Science.gov (United States)

    Castillo, Ricardo; Wang, Yeqin; Pol, Suhas; Swift, Andy; Hussain, Fazle; Westergaard, Carsten; Texas Tech University Team

    2016-11-01

    A wind tunnel based "Hyper Accelerated Wind Farm Kinematic-Control Simulator" (HAWKS) is being built at Texas Tech University to emulate controlled wind turbine flow physics. The HAWKS model turbine has pitch, yaw and speed control which is operated in real model time, similar to that of an equivalent full scale turbine. Also, similar to that of a full scale wind turbine, the controls are developed in a Matlab Simulink environment. The current diagnostic system consists of power, rotor position, rotor speed measurements and PIV wake characterization with four cameras. The setup allows up to 7D downstream of the rotor to be mapped. The purpose of HAWKS is to simulate control strategies at turnaround times much faster than CFD and full scale testing. The fundamental building blocks of the simulator have been tested, and demonstrate wake steering for both static and dynamic turbine actuation. Parameters which have been studied are yaw, rotor speed and combinations hereof. The measured wake deflections for static yaw cases are in agreement with previously reported research implying general applicability of the HAWKS platform for the purpose of manipulating the wake. In this presentation the general results will be introduced followed by an analysis of the wake turbulence and coherent structures when comparing static and dynamic flow cases. The outcome of such studies could ultimately support effective wind farm wake flow control strategies. Texas Emerging Technology Fund (ETF).

  3. Learning Dynamics for Robot Control under Varying Contexts

    OpenAIRE

    Petkos, Georgios

    2008-01-01

    High fidelity, compliant robot control requires a sufficiently accurate dynamics model. Often though, it is not possible to obtain a dynamics model sufficiently accurately or at all using analytical methods. In such cases, an alternative is to learn the dynamics model from movement data. This thesis discusses the problems specific to dynamics learning for control under nonstationarity of the dynamics. We refer to the cause of the nonstationarity as the context of the dynamics. ...

  4. Magnetic Field Control of Combustion Dynamics

    Science.gov (United States)

    Barmina, I.; Valdmanis, R.; Zake, M.; Kalis, H.; Marinaki, M.; Strautins, U.

    2016-08-01

    Experimental studies and mathematical modelling of the effects of magnetic field on combustion dynamics at thermo-chemical conversion of biomass are carried out with the aim of providing control of the processes developing in the reaction zone of swirling flame. The joint research of the magnetic field effect on the combustion dynamics includes the estimation of this effect on the formation of the swirling flame dynamics, flame temperature and composition, providing analysis of the magnetic field effects on the flame characteristics. The results of experiments have shown that the magnetic field exerts the influence on the flow velocity components by enhancing a swirl motion in the flame reaction zone with swirl-enhanced mixing of the axial flow of volatiles with cold air swirl, by cooling the flame reaction zone and by limiting the thermo-chemical conversion of volatiles. Mathematical modelling of magnetic field effect on the formation of the flame dynamics confirms that the electromagnetic force, which is induced by the electric current surrounding the flame, leads to field-enhanced increase of flow vorticity by enhancing mixing of the reactants. The magnetic field effect on the flame temperature and rate of reactions leads to conclusion that field-enhanced increase of the flow vorticity results in flame cooling by limiting the chemical conversion of the reactants.

  5. The BOXES Methodology Black Box Dynamic Control

    CERN Document Server

    Russell, David W

    2012-01-01

    Robust control mechanisms customarily require knowledge of the system’s describing equations which may be of the high order differential type.  In order to produce these equations, mathematical models can often be derived and correlated with measured dynamic behavior.  There are two flaws in this approach one is the level of inexactness introduced by linearizations and the other when no model is apparent.  Several years ago a new genre of control systems came to light that are much less dependent on differential models such as fuzzy logic and genetic algorithms. Both of these soft computing solutions require quite considerable a priori system knowledge to create a control scheme and sometimes complicated training program before they can be implemented in a real world dynamic system. Michie and Chambers’ BOXES methodology created a black box system that was designed to control a mechanically unstable system with very little a priori system knowledge, linearization or approximation.  All the method need...

  6. NSLS-II Control of Dynamic Aperture

    Energy Technology Data Exchange (ETDEWEB)

    Bengtsson,J.

    2008-10-31

    We have outlined how, by an intuitive approach, the on- and off-momentum dynamic aperture for a synchrotron light source can be estimated from a nonlinear system of algebraic equations for the sextupole/multipole strengths. The approach has only two free parameters: the relative weight for resonance vs. tune shift terms and the tune footprint for stable trajectories in a modern third generation synchrotron light source. In other words, we have established a control theory approach for the medium term (10{sup 3} turns) stability for a dynamic system described by a nonlinear system of ordinary differential equations. Equipped with a predictive, quantitative model for stability, we have then evaluated how to improve the control of the dynamics by analyzing and modifying the properties of the corresponding algebraic system. In particular, by changing the number- and characteristics of the parameters, i.e., we have not evaluated how the underlying (linear) optics could be improved. We have also validated our conjectures by numerical simulations with a realistic model. Presumably, our conclusions, summarized in Section 1.0, are a direct result of the presented analysis and observations.

  7. Nonlinear dynamics of cell orientation

    Science.gov (United States)

    Safran, S. A.; de, Rumi

    2009-12-01

    The nonlinear dependence of cellular orientation on an external, time-varying stress field determines the distribution of orientations in the presence of noise and the characteristic time, τc , for the cell to reach its steady-state orientation. The short, local cytoskeletal relaxation time distinguishes between high-frequency (nearly perpendicular) and low-frequency (random or parallel) orientations. However, τc is determined by the much longer, orientational relaxation time. This behavior is related to experiments for which we predict the angle and characteristic time as a function of frequency.

  8. Spacecraft Dynamics and Control Program at AFRPL

    Science.gov (United States)

    Das, A.; Slimak, L. K. S.; Schloegel, W. T.

    1986-01-01

    A number of future DOD and NASA spacecraft such as the space based radar will be not only an order of magnitude larger in dimension than the current spacecraft, but will exhibit extreme structural flexibility with very low structural vibration frequencies. Another class of spacecraft (such as the space defense platforms) will combine large physical size with extremely precise pointing requirement. Such problems require a total departure from the traditional methods of modeling and control system design of spacecraft where structural flexibility is treated as a secondary effect. With these problems in mind, the Air Force Rocket Propulsion Laboratory (AFRPL) initiated research to develop dynamics and control technology so as to enable the future large space structures (LSS). AFRPL's effort in this area can be subdivided into the following three overlapping areas: (1) ground experiments, (2) spacecraft modeling and control, and (3) sensors and actuators. Both the in-house and contractual efforts of the AFRPL in LSS are summarized.

  9. Attosecond VUV Coherent Control of Molecular Dynamics

    CERN Document Server

    Ranitovic, P; Riviere, P; Palacios, A; Tong, X M; Toshima, N; Gonzalez-Castrillo, A; Martin, L; Martin, F; Murnane, M M; Kapteyn, H C

    2014-01-01

    High harmonic light sources make it possible to access attosecond time-scales, thus opening up the prospect of manipulating electronic wave packets for steering molecular dynamics. However, two decades after the birth of attosecond physics, the concept of attosecond chemistry has not yet been realized. This is because excitation and manipulation of molecular orbitals requires precisely controlled attosecond waveforms in the deep ultraviolet, which have not yet been synthesized. Here, we present a novel approach using attosecond vacuum ultraviolet pulse-trains to coherently excite and control the outcome of a simple chemical reaction in a deuterium molecule in a non-Born Oppenheimer regime. By controlling the interfering pathways of electron wave packets in the excited neutral and singly-ionized molecule, we unambiguously show that we can switch the excited electronic state on attosecond timescales, coherently guide the nuclear wave packets to dictate the way a neutral molecule vibrates, and steer and manipula...

  10. Nonsmooth mechanics models, dynamics and control

    CERN Document Server

    Brogliato, Bernard

    2016-01-01

    Now in its third edition, this standard reference is a comprehensive treatment of nonsmooth mechanical systems refocused to give more prominence to control and modelling. It covers Lagrangian and Newton–Euler systems, detailing mathematical tools such as convex analysis and complementarity theory. The ways in which nonsmooth mechanics influence and are influenced by well-posedness analysis, numerical analysis and simulation, modelling and control are explained. Contact/impact laws, stability theory and trajectory-tracking control are given in-depth exposition connected by a framework formed from complementarity systems and measure-differential inclusions. Links are established with electrical circuits with set-valued nonsmooth elements and with other nonsmooth dynamical systems like impulsive and piecewise linear systems. Nonsmooth Mechanics (third edition) has been substantially rewritten, edited and updated to account for the significant body of results that have emerged in the twenty-first century—incl...

  11. Wnt signaling and stem cell control

    Institute of Scientific and Technical Information of China (English)

    Roel Nusse

    2008-01-01

    Wnt signaling has been implicated in the control over various types of stem cells and may act as a niche factor to maintain stem cells in a self-renewing state.As currently understood,Wnt proteins bind to receptors of the Frizzled and LRP families on the cell surface.Through several cytoplasmic relay components,the signal is transduced to B-catenin,which then enters the nucleus and forms a complex with TCF to activate transcription of Wnt target genes.Wnts can also signal through tyrosine kinase receptors,in particular the ROR and RYK receptors,leading to alternative modes of Wnt signaling.During the growth of tissues,these ligands and receptors are dynamically expressed,often transcriptionally controlled by Wnt signals themselves,to ensure the right balance between proliferation and differentiation.Isolated Wnt proteins are active on a variety of stem cells,including neural,mammary and embryonic stem cells.In general,Wnt proteins act to maintain the undifferentiated state of stem cells,while other growth factors instruct the cells to proliferate.These other factors include FGF and EGF,signaling through tyrosine kinase pathways.

  12. Intergenerational continuity of cell shape dynamics in Caulobacter crescentus

    Science.gov (United States)

    Wright, Charles S.; Banerjee, Shiladitya; Iyer-Biswas, Srividya; Crosson, Sean; Dinner, Aaron R.; Scherer, Norbert F.

    2015-03-01

    We investigate the intergenerational shape dynamics of single Caulobacter crescentus cells using a novel combination of imaging techniques and theoretical modeling. We determine the dynamics of cell pole-to-pole lengths, cross-sectional widths, and medial curvatures from high accuracy measurements of cell contours. Moreover, these shape parameters are determined for over 250 cells across approximately 10000 total generations, which affords high statistical precision. Our data and model show that constriction is initiated early in the cell cycle and that its dynamics are controlled by the time scale of exponential longitudinal growth. Based on our extensive and detailed growth and contour data, we develop a minimal mechanical model that quantitatively accounts for the cell shape dynamics and suggests that the asymmetric location of the division plane reflects the distinct mechanical properties of the stalked and swarmer poles. Furthermore, we find that the asymmetry in the division plane location is inherited from the previous generation. We interpret these results in terms of the current molecular understanding of shape, growth, and division of C. crescentus.

  13. The effect of applied control strategy on the current-voltage correlation of a solid oxide fuel cell stack during dynamic operation

    Directory of Open Access Journals (Sweden)

    Szmyd Janusz S.

    2014-09-01

    Full Text Available This paper discusses the transient characteristics of the planar type SOFC cell stack, of which the standard output is 300 W. The transient response of the voltage to the manipulation of an electric current was investigated. The effects of the response and of the operating condition determined by the operating temperature of the stack were studied by mapping a current-voltage (I-V correlation. The current-based fuel control (CBFC was adopted for keeping the fuel utilization factor at constant while the value of the electric current was ramped at the constant rate. The present experimental study shows that the transient characteristics of the cell voltage are determined by primarily the operating temperature caused by the manipulation of the current. Particularly, the slope of the I-V curve and the overshoot found on the voltage was remarkably influenced by the operating temperature. The different values of the fuel utilization factor influence the height of the settled voltages. The CBFC has significance in determining the slope of the I-V characteristic, but the different values ofthe fuel utilization factor does not affect the slope as the operating temperature does. The CBFC essentially does not alter the amplitude of the overshoot on the voltage response, since this is dominated by the operating temperature and its change is caused by manipulating the current.

  14. Control-volume representation of molecular dynamics.

    Science.gov (United States)

    Smith, E R; Heyes, D M; Dini, D; Zaki, T A

    2012-05-01

    A molecular dynamics (MD) parallel to the control volume (CV) formulation of fluid mechanics is developed by integrating the formulas of Irving and Kirkwood [J. Chem. Phys. 18, 817 (1950)] over a finite cubic volume of molecular dimensions. The Lagrangian molecular system is expressed in terms of an Eulerian CV, which yields an equivalent to Reynolds' transport theorem for the discrete system. This approach casts the dynamics of the molecular system into a form that can be readily compared to the continuum equations. The MD equations of motion are reinterpreted in terms of a Lagrangian-to-control-volume (LCV) conversion function ϑ(i) for each molecule i. The LCV function and its spatial derivatives are used to express fluxes and relevant forces across the control surfaces. The relationship between the local pressures computed using the volume average [Lutsko, J. Appl. Phys. 64, 1152 (1988)] techniques and the method of planes [Todd et al., Phys. Rev. E 52, 1627 (1995)] emerges naturally from the treatment. Numerical experiments using the MD CV method are reported for equilibrium and nonequilibrium (start-up Couette flow) model liquids, which demonstrate the advantages of the formulation. The CV formulation of the MD is shown to be exactly conservative and is, therefore, ideally suited to obtain macroscopic properties from a discrete system.

  15. Human T Cell Memory: A Dynamic View

    Directory of Open Access Journals (Sweden)

    Derek C. Macallan

    2017-02-01

    Full Text Available Long-term T cell-mediated protection depends upon the formation of a pool of memory cells to protect against future pathogen challenge. In this review we argue that looking at T cell memory from a dynamic viewpoint can help in understanding how memory populations are maintained following pathogen exposure or vaccination. For example, a dynamic view resolves the apparent paradox between the relatively short lifespans of individual memory cells and very long-lived immunological memory by focussing on the persistence of clonal populations, rather than individual cells. Clonal survival is achieved by balancing proliferation, death and differentiation rates within and between identifiable phenotypic pools; such pools correspond broadly to sequential stages in the linear differentiation pathway. Each pool has its own characteristic kinetics, but only when considered as a population; single cells exhibit considerable heterogeneity. In humans, we tend to concentrate on circulating cells, but memory T cells in non-lymphoid tissues and bone marrow are increasingly recognised as critical for immune defence; their kinetics, however, remain largely unexplored. Considering vaccination from this viewpoint shifts the focus from the size of the primary response to the survival of the clone and enables identification of critical system pinch-points and opportunities to improve vaccine efficacy.

  16. Collective dynamics of cell migration and cell rearrangements

    Science.gov (United States)

    Kabla, Alexandre

    Understanding multicellular processes such as embryo development or cancer metastasis requires to decipher the contributions of local cell autonomous behaviours and long range interactions with the tissue environment. A key question in this context concerns the emergence of large scale coordination in cell behaviours, a requirement for collective cell migration or convergent extension. I will present a few examples where physical and mechanical aspects play a significant role in driving tissue scale dynamics. Geometrical confinement is one of the key external factors influencing large scale coordination during collective migration. Using a combination of in vitro experiments and numerical simulations, we show that the velocity correlation length, measured in unconfined conditions, provides a convenient length scale to predict the dynamic response under confinement. The same length scale can also be used to quantify the influence range of directional cues within the cell population. Heterogeneity within motile cell populations is frequently associated with an increase in their invasive capability and appears to play an important role during cancer metastasis. Using in silico experiments, we studied the way cell invasion is influenced by both the degree of cell coordination and the amount of variability in the motile force of the invading cells. Results suggest that mechanical heterogeneity dramatically enhances the invasion rate through an emerging cooperative process between the stronger and weaker cells, accounting for a number of observed invasion phenotypes. Effective convergent extension requires on a consistent orientation of cell intercalation at the tissue scale, most often in relation with planar cell polarity mechanisms to define the primary axes of deformation. Using a novel modelling approach for cells mechanical interactions, we studied the dynamics of substrate free motile cell populations. Ongoing work shows in particular that nematic order emerges

  17. Advances in dynamical systems and control

    CERN Document Server

    Zgurovsky, Mikhail

    2016-01-01

    Focused on recent advances, this book covers theoretical foundations as well as various applications. It presents modern mathematical modeling approaches to the qualitative and numerical analysis of solutions for complex engineering problems in physics, mechanics, biochemistry, geophysics, biology and climatology. Contributions by an international team of respected authors bridge the gap between abstract mathematical approaches, such as applied methods of modern analysis, algebra, fundamental and computational mechanics, nonautonomous and stochastic dynamical systems on the one hand, and practical applications in nonlinear mechanics, optimization, decision making theory and control theory on the other. As such, the book will be of interest to mathematicians and engineers working at the interface of these fields. .

  18. Cell biology. Metabolic control of cell death.

    Science.gov (United States)

    Green, Douglas R; Galluzzi, Lorenzo; Kroemer, Guido

    2014-09-19

    Beyond their contribution to basic metabolism, the major cellular organelles, in particular mitochondria, can determine whether cells respond to stress in an adaptive or suicidal manner. Thus, mitochondria can continuously adapt their shape to changing bioenergetic demands as they are subjected to quality control by autophagy, or they can undergo a lethal permeabilization process that initiates apoptosis. Along similar lines, multiple proteins involved in metabolic circuitries, including oxidative phosphorylation and transport of metabolites across membranes, may participate in the regulated or catastrophic dismantling of organelles. Many factors that were initially characterized as cell death regulators are now known to physically or functionally interact with metabolic enzymes. Thus, several metabolic cues regulate the propensity of cells to activate self-destructive programs, in part by acting on nutrient sensors. This suggests the existence of "metabolic checkpoints" that dictate cell fate in response to metabolic fluctuations. Here, we discuss recent insights into the intersection between metabolism and cell death regulation that have major implications for the comprehension and manipulation of unwarranted cell loss.

  19. Dynamic analysis of magnetic nanoparticles crossing cell membrane

    Science.gov (United States)

    Pedram, Maysam Z.; Shamloo, Amir; Ghafar-Zadeh, Ebrahim; Alasty, Aria

    2017-01-01

    Nowadays, nanoparticles (NPs) are used in a variety of biomedical applications including brain disease diagnostics and subsequent treatments. Among the various types of NPs, magnetic nanoparticles (MNPs) have been implemented by many research groups for an array of life science applications. In this paper, we studied MNPs controlled delivery into the endothelial cells using a magnetic field. Dynamics equations of MNPs were defined in the continuous domain using control theory methods and were applied to crossing the cell membrane. This study, dedicated to clinical and biomedical research applications, offers a guideline for the generation of a magnetic field required for the delivery of MNPs.

  20. Neural network based dynamic controllers for industrial robots.

    Science.gov (United States)

    Oh, S Y; Shin, W C; Kim, H G

    1995-09-01

    The industrial robot's dynamic performance is frequently measured by positioning accuracy at high speeds and a good dynamic controller is essential that can accurately compute robot dynamics at a servo rate high enough to ensure system stability. A real-time dynamic controller for an industrial robot is developed here using neural networks. First, an efficient time-selectable hidden layer architecture has been developed based on system dynamics localized in time, which lends itself to real-time learning and control along with enhanced mapping accuracy. Second, the neural network architecture has also been specially tuned to accommodate servo dynamics. This not only facilitates the system design through reduced sensing requirements for the controller but also enhances the control performance over the control architecture neglecting servo dynamics. Experimental results demonstrate the controller's excellent learning and control performances compared with a conventional controller and thus has good potential for practical use in industrial robots.

  1. Dynamics of B cells in germinal centres.

    Science.gov (United States)

    De Silva, Nilushi S; Klein, Ulf

    2015-03-01

    Humoral immunity depends on the germinal centre (GC) reaction during which somatically mutated high-affinity memory B cells and plasma cells are generated. Recent studies have uncovered crucial cues that are required for the formation and the maintenance of GCs and for the selection of high-affinity antibody mutants. In addition, it is now clear that these events are promoted by the dynamic movements of cells within and between GCs. These findings have resolved the complexities of the GC reaction in greater detail than ever before. This Review focuses on these recent advances and discusses their implications for the establishment of humoral immunity.

  2. Dynamics and control of morphing aircraft

    Science.gov (United States)

    Seigler, Thomas Michael

    The following work is directed towards an evaluation of aircraft that undergo structural shape change for the purpose of optimized flight and maneuvering control authority. Dynamical equations are derived for a morphing aircraft based on two primary representations; a general non-rigid model and a multi-rigid-body. A simplified model is then proposed by considering the altering structural portions to be composed of a small number of mass particles. The equations are then extended to consider atmospheric flight representations where the longitudinal and lateral equations are derived. Two aspects of morphing control are considered. The first is a regulation problem in which it is desired to maintain stability in the presence of large changes in both aerodynamic and inertial properties. From a baseline aircraft model various wing planform designs were constructed using Datcom to determine the required aerodynamic contributions. Based on nonlinear numerical evaluations adequate stabilization control was demonstrated using a robust linear control design. In maneuvering, divergent characteristics were observed at high structural transition rates. The second aspect considered is the use of structural changes for improved flight performance. A variable span aircraft is then considered in which asymmetric wing extension is used to effect the rolling moment. An evaluation of the variable span aircraft is performed in the context of bank-to-turn guidance in which an input-output control law is implemented.

  3. Dynamic Discontinuous Control for Active Control of Mechanical Vibrations

    Directory of Open Access Journals (Sweden)

    Orestes Llanes Santiago

    2010-02-01

    Full Text Available This article shows the use of the discontinuous control using dynamic sliding modes for the active isolation of vibrations in mechanical systems. This type of control law constitutes a robust feedback control policy due to its insensitivity to external disturbance inputs, certain immunity to model parameter variations, within known bounds, and to the ever present modelling errors.  The whole theoretical analysis is applied to a lineal model of two degrees of freedom of the vehicle's suspension where the irregularities of the land represent of direct  way the external interferences to the system . To carry out the isolation an electro-hydraulic operator it is used. Simulations are performed which validate the proposed approach.

  4. NASA Armstrong Flight Research Center Dynamics and Controls Branch

    Science.gov (United States)

    Jacobson, Steve

    2015-01-01

    NASA Armstrong continues its legacy of exciting work in the area of Dynamics and Control of advanced vehicle concepts. This presentation describes Armstrongs research in control of flexible structures, peak seeking control and adaptive control in the Spring of 2015.

  5. Dynamic congestion control mechanisms for MPLS networks

    Science.gov (United States)

    Holness, Felicia; Phillips, Chris I.

    2001-02-01

    Considerable interest has arisen in congestion control through traffic engineering from the knowledge that although sensible provisioning of the network infrastructure is needed, together with sufficient underlying capacity, these are not sufficient to deliver the Quality of Service required for new applications. This is due to dynamic variations in load. In operational Internet Protocol (IP) networks, it has been difficult to incorporate effective traffic engineering due to the limited capabilities of the IP technology. In principle, Multiprotocol Label Switching (MPLS), which is a connection-oriented label swapping technology, offers new possibilities in addressing the limitations by allowing the operator to use sophisticated traffic control mechanisms. This paper presents a novel scheme to dynamically manage traffic flows through the network by re-balancing streams during periods of congestion. It proposes management-based algorithms that will allow label switched routers within the network to utilize mechanisms within MPLS to indicate when flows are starting to experience frame/packet loss and then to react accordingly. Based upon knowledge of the customer's Service Level Agreement, together with instantaneous flow information, the label edge routers can then instigate changes to the LSP route and circumvent congestion that would hitherto violate the customer contacts.

  6. Spacecraft formation flying: Dynamics, control and navigation

    Science.gov (United States)

    Alfriend, Kyle Terry; Vadali, Srinivas Rao; Gurfil, Pini; How, Jonathan; Breger, Louis S.

    2009-12-01

    Space agencies are now realizing that much of what has previously been achieved using hugely complex and costly single platform projects - large unmanned and manned satellites (including the present International Space Station) - can be replaced by a number of smaller satellites networked together. The key challenge of this approach, namely ensuring the proper formation flying of multiple craft, is the topic of this second volume in Elsevier's Astrodynamics Series, Spacecraft Formation Flying: Dynamics, control and navigation. In this unique text, authors Alfriend et al. provide a coherent discussion of spacecraft relative motion, both in the unperturbed and perturbed settings, explain the main control approaches for regulating relative satellite dynamics, using both impulsive and continuous maneuvers, and present the main constituents required for relative navigation. The early chapters provide a foundation upon which later discussions are built, making this a complete, standalone offering. Intended for graduate students, professors and academic researchers in the fields of aerospace and mechanical engineering, mathematics, astronomy and astrophysics, Spacecraft Formation Flying is a technical yet accessible, forward-thinking guide to this critical area of astrodynamics.

  7. Dynamic heterogeneity and DNA methylation in embryonic stem cells.

    KAUST Repository

    Singer, Zakary S

    2014-07-01

    Cell populations can be strikingly heterogeneous, composed of multiple cellular states, each exhibiting stochastic noise in its gene expression. A major challenge is to disentangle these two types of variability and to understand the dynamic processes and mechanisms that control them. Embryonic stem cells (ESCs) provide an ideal model system to address this issue because they exhibit heterogeneous and dynamic expression of functionally important regulatory factors. We analyzed gene expression in individual ESCs using single-molecule RNA-FISH and quantitative time-lapse movies. These data discriminated stochastic switching between two coherent (correlated) gene expression states and burst-like transcriptional noise. We further showed that the "2i" signaling pathway inhibitors modulate both types of variation. Finally, we found that DNA methylation plays a key role in maintaining these metastable states. Together, these results show how ESC gene expression states and dynamics arise from a combination of intrinsic noise, coherent cellular states, and epigenetic regulation.

  8. Controls of interaction dynamics of orbital assembly

    Science.gov (United States)

    Su, Renjeng

    1991-01-01

    Building structures and spacecraft in orbit will require technologies for positioning, docking/berthing, and joining orbital structures. A fundamental problem underlying the operation of docking and berthing is that of controlling the contact dynamics of mechanical structures actuated by active mechanisms such as robotic devices. Control systems must be designed to control these active mechanisms so that both the free space motions and contact motions are stable and satisfy specifications on position accuracy and bounds on contact forces. For the large orbital structures of the future, the problem of interactive dynamics and control is fundamentally different in several ways than it was for spacecraft docking in the past. First, future space structures must be treated as flexible structures - the operations of docking, berthing, and assembly will need to respect the vibrations of the structures. Second, the assembly of these structures will require multiple-point contact, rather than the essentially single-point positioning of conventional spacecraft docking. Third, some assembly operations require the subassemblies to be brought and held in contact so that successful joining can be accomplished. A preliminary study of contact stability and compliance control design has resulted in the development of an analytical method and a design method to analyze stability. The analytical method analyzes the problem of stability when an actively-controlled structure contacts a passive structure. This method makes it possible to accurately estimate the stiffness of the passive structures with which the contact motion will become unstable. The analytic results suggest that passivity is neither achievable in practice, nor necessary as a design concept. A contact control system need only be passive up to a certain frequency; beyond that frequency the system can be stabilized with sufficiently small gains. With this concept the Center developed a design methodology for achieving

  9. Electron dynamics and its control in molecules

    Science.gov (United States)

    de Vivie-Riedle, Regina

    2014-03-01

    The accessibility of few femtosecond or even attoseconds pulses opens the door to direct observation of electron dynamics. The idea to steer chemical reactions by localization of electronic wavepackets is intriguing, since electrons are directly involved in bond breaking and formation. The formation of a localized electronic wavepacket requires the superposition of two or more appropriate electronic states. Its guidance is only possible within the coherence time of the system and has to be synchronized with the vibrational molecular motions. In theoretical studies we elucidate the role of electron wavepacket motion for the control of molecular processes. We give three examples with direct connection to experiments. From our analysis, we extract the systems requirements defining the time window for intramolecular electronic coherence, the basis for efficient control. Based on these findings we map out a photoreaction that allows direct control by guiding electronic wavepackets. The carrier envelope of a femtosecond few cycle IR pulse is the control parameter that steers the photoreaction through a conical intersection.

  10. Adaptive control of force microscope cantilever dynamics

    Science.gov (United States)

    Jensen, S. E.; Dougherty, W. M.; Garbini, J. L.; Sidles, J. A.

    2007-09-01

    Magnetic resonance force microscopy (MRFM) and other emerging scanning probe microscopies entail the detection of attonewton-scale forces. Requisite force sensitivities are achieved through the use of soft force microscope cantilevers as high resonant-Q micromechanical oscillators. In practice, the dynamics of these oscillators are greatly improved by the application of force feedback control computed in real time by a digital signal processor (DSP). Improvements include increased sensitive bandwidth, reduced oscillator ring up/down time, and reduced cantilever thermal vibration amplitude. However, when the cantilever tip and the sample are in close proximity, electrostatic and Casimir tip-sample force gradients can significantly alter the cantilever resonance frequency, foiling fixed-gain narrow-band control schemes. We report an improved, adaptive control algorithm that uses a Hilbert transform technique to continuously measure the vibration frequency of the thermally-excited cantilever and seamlessly adjust the DSP program coefficients. The closed-loop vibration amplitude is typically 0.05 nm. This adaptive algorithm enables narrow-band formally-optimal control over a wide range of resonance frequencies, and preserves the thermally-limited signal to noise ratio (SNR).

  11. Optimizing Dynamical Network Structure for Pinning Control

    Science.gov (United States)

    Orouskhani, Yasin; Jalili, Mahdi; Yu, Xinghuo

    2016-04-01

    Controlling dynamics of a network from any initial state to a final desired state has many applications in different disciplines from engineering to biology and social sciences. In this work, we optimize the network structure for pinning control. The problem is formulated as four optimization tasks: i) optimizing the locations of driver nodes, ii) optimizing the feedback gains, iii) optimizing simultaneously the locations of driver nodes and feedback gains, and iv) optimizing the connection weights. A newly developed population-based optimization technique (cat swarm optimization) is used as the optimization method. In order to verify the methods, we use both real-world networks, and model scale-free and small-world networks. Extensive simulation results show that the optimal placement of driver nodes significantly outperforms heuristic methods including placing drivers based on various centrality measures (degree, betweenness, closeness and clustering coefficient). The pinning controllability is further improved by optimizing the feedback gains. We also show that one can significantly improve the controllability by optimizing the connection weights.

  12. Dynamic synchrony of local cell assembly.

    Science.gov (United States)

    Sakurai, Yoshio; Takahashi, Susumu

    2008-01-01

    In the present paper, we focus on the problem of the dynamic size of a cell assembly and discuss how we can detect synchronized firing of a local cell assembly consisting of closely neighboring neurons in the working brain. A local cell assembly is difficult to detect because of the problem of spike overlapping of neighboring neurons, which cannot be overcome by ordinary spike-sorting techniques. We introduce a unique technique of spike-sorting that combines independent component analysis (ICA) and an ordinary sorting method to separate individual neighboring neurons and analyze their firing synchrony in behaving animals. One of our experiments employing this method showed that some closely neighboring neurons in the monkey prefrontal cortex have dynamic and sharp synchrony of firing reflecting local cell assemblies during working-memory processes. Another experiment showed that our other method (ICSort) of novel spike-sorting by ICA using special electrodes (dodecatrodes) can distinguish firing signals from the soma and those from the dendrites of individual neurons in behaving rats and suggests that the somatic and dendritic signals have different roles in information processing. This indicates that functional connectivity among neurons may be more dynamic and complex and spikes from the soma and dendrites of individual neurons should be considered in the investigation of the activity of local cell assemblies. We finally propose that detailed and real features of a local cell assembly consisting of closely neighboring neurons should be examined further and detection of local cell assemblies could be applied to the development of neuronal prosthetic devices, that is, brain-machine interfaces (BMIs).

  13. Approximate Dynamic Programming for Self-Learning Control

    Institute of Scientific and Technical Information of China (English)

    Derong Liu

    2005-01-01

    This paper introduces a self-learning control approach based on approximate dynamic programming. Dynamic programming was introduced by Bellman in the 1950's for solving optimal control problems of nonlinear dynamical systems. Due to its high computational complexity, the applications of dynamic programming have been limited to simple and small problems. The key step in finding approximate solutions to dynamic programming is to estimate the performance index in dynamic programming. The optimal control signal can then be determined by minimizing (or maximizing) the performance index. Artificial neural networks are very efficient tools in representing the performance index in dynamic programming. This paper assumes the use of neural networks for estimating the performance index in dynamic programming and for generating optimal control signals, thus to achieve optimal control through self-learning.

  14. Dynamic control of knee axial deformities

    Directory of Open Access Journals (Sweden)

    E. E. Malyshev

    2013-01-01

    Full Text Available The authors have evaluated the clinical examination of the patients with axial malalignments in the knee by the original method and device which was named varovalgometer. The measurements were conducted by tension of the cord through the spina iliaca anterior superior and the middle of the lower pole of patella. The deviation of the center of the ankle estimated by metal ruler which was positioned perpendicular to the lower leg axis on the level of the ankle joint line. The results of comparison of our method and computer navigation in 53 patients during the TKA show no statistically significant varieties but they differ by average 5° of valgus in clinical examination in comparison with mechanical axis which was identified by computer navigation. The dynamic control of axial malalignment can be used in clinical practice for estimation of the results of treatment of pathology with axial deformities in the knee; for the control of reduction and secondary displacement of the fractures around the knee; for assessment of instability; in planning of correctional osteotomies and intraoperative control of deformity correction; for estimation of Q angle in subluxation and recurrent dislocation of patella; in planning of TKA; during the growth of child it allows to assess the progression of deformity.

  15. Modeling cell dynamics under mobile phone radiation.

    Science.gov (United States)

    Minelli, Tullio Antonio; Balduzzo, Maurizio; Milone, Francesco Ferro; Nofrate, Valentina

    2007-04-01

    Perturbations by pulse-modulated microwave radiation from GSM mobile phones on neuron cell membrane gating and calcium oscillations have been suggested as a possible mechanism underlying activation of brain states and electroencephalographic epiphenomena. As the employ of UMTS phones seems to reveal other symptoms, a unified phenomenological framework is needed. In order to explain possible effects of mobile phone radiation on cell oscillations, GSM and UMTS low-frequency envelopes have been detected, recorded and used as input in cell models. Dynamical systems endowed with contiguous regular and chaotic regimes suitable to produce stochastic resonance can both account for the perturbation of the neuro-electrical activity and even for the low intensity of the signal perceived by high sensitive subjects. Neuron models of this kind can be employed as a reductionist hint for the mentioned phenomenology. The Hindmarsh-Rose model exhibits frequency enhancement and regularization phenomena induced by weak GSM and UMTS. More realistic simulations of cell membrane gating and calcium oscillations have been performed with the help of an adaptation of the Chay-Keizer dynamical system. This scheme can explain the suspected subjective sensitivity to mobile phone signals under the thermal threshold, in terms of cell calcium regularity mechanisms. Concerning the two kinds of emission, the stronger occupation of the ELF band of last generation UMTS phones is compensated by lower power emitted.

  16. Triggering cell adhesion, migration or shape change with a dynamic surface coating.

    Science.gov (United States)

    van Dongen, Stijn F M; Maiuri, Paolo; Marie, Emmanuelle; Tribet, Christophe; Piel, Matthieu

    2013-03-25

    There's an APP for that: cell-repellent APP (azido-[polylysine-g-PEG]) is used to create substrates for spatially controlled dynamic cell adhesion. The simple addition of a functional peptide to the culture medium rapidly triggers cell adhesion. This highly accessible yet powerful technique allows diverse applications, demonstrated through tissue motility assays, patterned coculturing and triggered cell shape change.

  17. Modeling Robot Dynamic Performance for Endpoint Force Control

    Science.gov (United States)

    1988-08-01

    Task Dynamics 55 2.5.1 The Dynamic Workpiece Model 55 2.5.2 Adding Robot Dynamics 56 2.5.3 Adding Actuator Dynamics 56 Tabie I o iiau 6 2.6 Grip...motion control system. Robot dynamics couple with the task dynamics in a very complex way. When the robot makes contact with the environment, the impact...robot flexibility or actuator dynamics. 2.5.2 Adding Robot Dynamics Figure 2.29 shows the robot now represented by two lumped masses, as in the robot

  18. DYNAMIC CONGESTION CONTROL IN WDM OPTICAL NETWORK

    Directory of Open Access Journals (Sweden)

    Sangita Samajpati

    2013-02-01

    Full Text Available This paper is based on Wavelength Division Multiplexing (WDM optical networking. In this optical networking, prior to data transfer, lightpath establishment between source and destination nodes is usually carried out through a wavelength reservation protocol. This wavelength is reserved corresponding to a route between the source and destination and the route is chosen following any standard routing protocol based on shortest path. The backward reservation protocol is implemented initially. A fixed connected and weighted network is considered. The inputs of this implementation are the fixed network itself and its corresponding shortest path matrix. After this initial level of implementation, the average node usage over a time period is calculated and various thresholds for node usage are considered. Above threshold value, request arriving at that path selects its next shortest path. This concept is implemented on various wavelengths. The output represents the performance issues of dynamic congestion control.

  19. A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids.

    Science.gov (United States)

    Massai, Diana; Isu, Giuseppe; Madeddu, Denise; Cerino, Giulia; Falco, Angela; Frati, Caterina; Gallo, Diego; Deriu, Marco A; Falvo D'Urso Labate, Giuseppe; Quaini, Federico; Audenino, Alberto; Morbiducci, Umberto

    2016-01-01

    A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydrodynamics establishing within the culture chamber enabling dynamic cell suspension in an environment favourable to mass transport, under a wide range of tunable shear stress conditions. The design phase of the device has been supported by multiphysics modelling and has provided a comprehensive analysis of the operating principles of the bioreactor. Moreover, an explanatory example is herein presented with multiphysics simulations used to set the proper bioreactor operating conditions for preliminary in vitro biological tests on a human lung carcinoma cell line. The biological results demonstrate that the ultralow shear dynamic suspension provided by the device is beneficial for culturing cancer cell spheroids. In comparison to the static suspension control, dynamic cell suspension preserves morphological features, promotes intercellular connection, increases spheroid size (2.4-fold increase) and number of cycling cells (1.58-fold increase), and reduces double strand DNA damage (1.5-fold reduction). It is envisioned that the versatility of this bioreactor could allow investigation and expansion of different cell types in the future.

  20. Spatiotemporal control of cardiac anisotropy using dynamic nanotopographic cues.

    Science.gov (United States)

    Mengsteab, Paulos Y; Uto, Koichiro; Smith, Alec S T; Frankel, Sam; Fisher, Elliot; Nawas, Zeid; Macadangdang, Jesse; Ebara, Mitsuhiro; Kim, Deok-Ho

    2016-04-01

    Coordinated extracellular matrix spatiotemporal reorganization helps regulate cellular differentiation, maturation, and function in vivo, and is therefore vital for the correct formation, maintenance, and healing of complex anatomic structures. In order to evaluate the potential for cultured cells to respond to dynamic changes in their in vitro microenvironment, as they do in vivo, the collective behavior of primary cardiac muscle cells cultured on nanofabricated substrates with controllable anisotropic topographies was studied. A thermally induced shape memory polymer (SMP) was employed to assess the effects of a 90° transition in substrate pattern orientation on the contractile direction and structural organization of cardiomyocyte sheets. Cardiomyocyte sheets cultured on SMPs exhibited anisotropic contractions before shape transition. 48 h after heat-induced shape transition, the direction of cardiomyocyte contraction reoriented significantly and exhibited a bimodal distribution, with peaks at ∼45 and -45° (P < 0.001). Immunocytochemical analysis highlighted the significant structural changes that the cells underwent in response to the shift in underlying topography. The presented results demonstrate that initial anisotropic nanotopographic cues do not permanently determine the organizational fate or contractile properties of cardiomyocytes in culture. Given the importance of surface cues in regulating primary and stem cell development, investigation of such tunable nanotopographies may have important implications for advancing cellular maturation and performance in vitro, as well as improving our understanding of cellular development in response to dynamic biophysical cues.

  1. Dynamics and control of tethered spacecraft - A brief overview

    Science.gov (United States)

    Modi, V. J.; Lakshmanan, P. K.; Misra, A. K.

    1990-01-01

    Work related to the dynamics of tether connected orbiting systems and their control during deployment, operational, and retrieval phases is briefly reviewed. In particular, attention is given to the modeling of tether dynamics and control, end bodies tethered at point masses, deployment dynamics and tension control, and thruster and offset control. Directions of future efforts aimed at gaining a better understanding of the performance of tethered systems are outlined.

  2. Epigenetic control of cell identity and plasticity

    KAUST Repository

    Orlando, Valerio

    2014-04-02

    The DNA centered dogma for genetic information and cell identity is now evolving into a much more complex and flexible dimension provided by the discovery of the Epigenome. This comprises those chromosome structural and topological components that complement DNA information and contribute to genome functional organization. Current concept is that the Epigenome constitutes the dynamic molecular interface allowing the Genome to interact with the Environment. Exploring how the genome interacts with the environment is a key to fully understand cellular and complex organism mechanisms of adaptation and plasticity. Our work focuses on the role of an essential, specialized group or chromatin associated proteins named Polycomb (PcG) that control maintenance of transcription programs during development and in adult life. In particular PcG proteins exert epigenetic “memory” function by modifying chromosome structures at various levels to maintain gene silencing in particular through cell division. While in the past decade substantial progress was made in understanding PcG mechanisms acting in development and partially during cell cycle, very little is known about their role in adult post-mitotic tissues and more in general the role of the epigenome in adaptation. To this, we studied the role of PcG in the context of mammalian skeletal muscle cell differentiation. We previously reported specific dynamics of PRC2 proteins in myoblasts and myotubes, in particular the dynamics of PcG Histone H3 K27 Methyl Transferases (HMT), EZH2 and EZH1, the latter apparently replacing for EZH2 in differentiated myotubes. Ezh1 protein, although almost identical to Ezh2, shows a weak H3K27 HMT activity and its primary function remains elusive. Recent ChIPseq studies performed in differentiating muscle cells revealed that Ezh1 associates with active and not repressed regulatory regions to control RNA pol II elongation. Since H3K27 tri-methylation levels are virtually steady in non

  3. Stochastic dynamics of interacting haematopoietic stem cell niche lineages.

    Directory of Open Access Journals (Sweden)

    Tamás Székely

    2014-09-01

    Full Text Available Since we still know very little about stem cells in their natural environment, it is useful to explore their dynamics through modelling and simulation, as well as experimentally. Most models of stem cell systems are based on deterministic differential equations that ignore the natural heterogeneity of stem cell populations. This is not appropriate at the level of individual cells and niches, when randomness is more likely to affect dynamics. In this paper, we introduce a fast stochastic method for simulating a metapopulation of stem cell niche lineages, that is, many sub-populations that together form a heterogeneous metapopulation, over time. By selecting the common limiting timestep, our method ensures that the entire metapopulation is simulated synchronously. This is important, as it allows us to introduce interactions between separate niche lineages, which would otherwise be impossible. We expand our method to enable the coupling of many lineages into niche groups, where differentiated cells are pooled within each niche group. Using this method, we explore the dynamics of the haematopoietic system from a demand control system perspective. We find that coupling together niche lineages allows the organism to regulate blood cell numbers as closely as possible to the homeostatic optimum. Furthermore, coupled lineages respond better than uncoupled ones to random perturbations, here the loss of some myeloid cells. This could imply that it is advantageous for an organism to connect together its niche lineages into groups. Our results suggest that a potential fruitful empirical direction will be to understand how stem cell descendants communicate with the niche and how cancer may arise as a result of a failure of such communication.

  4. Laminar separation bubbles: Dynamics and control

    Indian Academy of Sciences (India)

    Sourabh S Diwan; O N Ramesh

    2007-02-01

    This work is an experimental investigation of the dynamics and control of the laminar separation bubbles which are typically present on the suction surface of an aerofoil at a large angle of attack. A separation bubble is produced on the upper surface of a flat plate by appropriately contouring the top wall of the wind tunnel. First, a basic (unforced) separation bubble is obtained to set a benchmark for further experiments. Parametric study is done where the reference velocity is decreased to quantify its effect on the aspect ratio of the bubble. It is found that with decrease in Reynolds number, the height of the bubble increases at a greater rate than the length. This feature could be useful in characterising separation bubbles especially from the point of view of low Reynolds number aerofoil design. Artificial disturbance is introduced at two different initial amplitudes (infinitesimal and finite) upstream of separation location and hotwire anemometry is used to trace the wave packet as it is advected downstream. The evolution of wave packets is seen to take place in two distinct stages. Finite amplitude forcing causes periodic quenching of the bubble. Interestingly, even an infinitesimally small forcing is seen to modify and thereby control the separation bubble.

  5. Stochastic and coherent dynamics of single and coupled beta cells

    DEFF Research Database (Denmark)

    phenomenon, modeled by a slow-fast nonlinear system of ordinary differential equations (ODEs). The single cell oscillations are complex as the dynamical behavior is a result of traversing a series of saddle node and homoclinic bifurcations, controlled by the slow variable. We shall present results...... is the simplest reaction-diffusion partial differential equation....... on the burst period as function of an external applied stochastic term and use a technique for reducing the stochastic differential equations to ODEs for the average and higher order moments. The later method is approximate and we shall discuss the limits of validity. The individual beta cells are coupled...

  6. Dynamic Response during PEM Fuel Cell Loading-up

    OpenAIRE

    Jun Gou; Pengcheng Li; Xing Yuan; Pucheng Pei

    2009-01-01

    A study on the effects of controlling and operating parameters for a Proton Exchange Membrane (PEM) fuel cell on the dynamic phenomena during the loading-up process is presented. The effect of the four parameters of load-up amplitudes and rates, operating pressures and current levels on gas supply or even starvation in the flow field is analyzed based accordingly on the transient characteristics of current output and voltage. Experiments are carried out in a single fuel cell with an active ar...

  7. Computer control of a microgravity mammalian cell bioreactor

    Science.gov (United States)

    Hall, William A.

    1987-01-01

    The initial steps taken in developing a completely menu driven and totally automated computer control system for a bioreactor are discussed. This bioreactor is an electro-mechanical cell growth system cell requiring vigorous control of slowly changing parameters, many of which are so dynamically interactive that computer control is a necessity. The process computer will have two main functions. First, it will provide continuous environmental control utilizing low signal level transducers as inputs and high powered control devices such as solenoids and motors as outputs. Secondly, it will provide continuous environmental monitoring, including mass data storage and periodic data dumps to a supervisory computer.

  8. Microtubule dynamics control HGF-induced lung endothelial barrier enhancement.

    Directory of Open Access Journals (Sweden)

    Xinyong Tian

    Full Text Available Microtubules (MT play a vital role in many cellular functions, but their role in peripheral actin cytoskeletal dynamics which is essential for control of endothelial barrier and monolayer integrity is less understood. We have previously described the enhancement of lung endothelial cell (EC barrier by hepatocyte growth factor (HGF which was associated with Rac1-mediated remodeling of actin cytoskeleton. This study investigated involvement of MT-dependent mechanisms in the HGF-induced enhancement of EC barrier. HGF-induced Rac1 activation was accompanied by phosphorylation of stathmin, a regulator of MT dynamics. HGF also stimulated MT peripheral growth monitored by time lapse imaging and tracking analysis of EB-1-decorated MT growing tips, and increased the pool of acetylated tubulin. These effects were abolished by EC pretreatment with HGF receptor inhibitor, downregulation of Rac1 pathway, or by expression of a stathmin-S63A phosphorylation deficient mutant. Expression of stathmin-S63A abolished the HGF protective effects against thrombin-induced activation of RhoA cascade, permeability increase, and EC barrier dysfunction. These results demonstrate a novel MT-dependent mechanism of HGF-induced EC barrier regulation via Rac1/PAK1/stathmin-dependent control of MT dynamics.

  9. Characterizing motility dynamics in human RPE cells

    Science.gov (United States)

    Liu, Zhuolin; Kurokawa, Kazuhiro; Zhang, Furu; Miller, Donald T.

    2017-02-01

    Retinal pigment epithelium (RPE) cells are vital to health of the outer retina, however, are often compromised in ageing and ocular diseases that lead to blindness. Early manifestation of RPE disruption occurs at the cellular level, but while in vivo biomarkers at this scale hold considerable promise, RPE cells have proven extremely challenging to image in the living human eye. Recently we addressed this problem by using organelle motility as a novel contrast agent to enhance the RPE cell in conjunction with 3D resolution of adaptive optics-optical coherence tomography (AO-OCT) to section the RPE layer. In this study, we expand on the central novelty of our method - organelle motility - by characterizing the dynamics of the motility in individual RPE cells, important because of its direct link to RPE physiology. To do this, AO-OCT videos of the same retinal patch were acquired at approximately 1 min intervals or less, time stamped, and registered in 3D with sub-cellular accuracy. Motility was quantified by an exponential decay time constant, the time for motility to decorrelate the speckle field across an RPE cell. In two normal subjects, we found the decay time constant to be just 3 seconds, thus indicating rapid motility in normal RPE cells.

  10. Muscle Stem Cell Fate Is Controlled by the Cell-Polarity Protein Scrib

    Directory of Open Access Journals (Sweden)

    Yusuke Ono

    2015-02-01

    Full Text Available Satellite cells are resident skeletal muscle stem cells that supply myonuclei for homeostasis, hypertrophy, and repair in adult muscle. Scrib is one of the major cell-polarity proteins, acting as a potent tumor suppressor in epithelial cells. Here, we show that Scrib also controls satellite-cell-fate decisions in adult mice. Scrib is undetectable in quiescent cells but becomes expressed during activation. Scrib is asymmetrically distributed in dividing daughter cells, with robust accumulation in cells committed to myogenic differentiation. Low Scrib expression is associated with the proliferative state and preventing self-renewal, whereas high Scrib levels reduce satellite cell proliferation. Satellite-cell-specific knockout of Scrib in mice causes a drastic and insurmountable defect in muscle regeneration. Thus, Scrib is a regulator of tissue stem cells, controlling population expansion and self-renewal with Scrib expression dynamics directing satellite cell fate.

  11. Equivalence Between Approximate Dynamic Inversion and Proportional-Integral Control

    Science.gov (United States)

    2008-09-29

    Hovakimyan, E. Lavretsky, and C. Cao, “Dynamic inversion of multi- input nonaffine systems via time-scale separation,” in Proceedings of the American Control Conference , Minneapolis...Adaptive dynamic inversion for nonaffine-in-control systems via time-scale separation: Part II,” in Proceedings of the American Control Conference , Portland

  12. Efficient Quantum Private Communication Based on Dynamic Control Code Sequence

    Science.gov (United States)

    Cao, Zheng-Wen; Feng, Xiao-Yi; Peng, Jin-Ye; Zeng, Gui-Hua; Qi, Jin

    2016-12-01

    Based on chaos and quantum properties, we propose a quantum private communication scheme with dynamic control code sequence. The initial sequence is obtained via chaotic systems, and the control code sequence is derived by grouping, XOR and extracting. A shift cycle algorithm is designed to enable the dynamic change of control code sequence. Analysis shows that transmission efficiency could reach 100 % with high dynamics and security.

  13. Impulsive Stabilization of Uncertain Dynamical Systems and Chaos Control

    Institute of Scientific and Technical Information of China (English)

    LIUBin; YAOJian; FANGJinqing; LIUXinzhi

    2004-01-01

    In this paper, a general impulsive control problem for uncertain dynamical systems is investigated.By utilizing the method of Lyapunov functions, a set of stability criteria for uncertain impulsive dynamical systems are established. These obtained results are then appliedto derive conditions under which an uncertain dynamical system can be robustly stabilized by an impulsive control law. Finally, we demonstrate our method by controlling the famous Lorenz system with uncertain perturbation.

  14. Efficient Quantum Private Communication Based on Dynamic Control Code Sequence

    Science.gov (United States)

    Cao, Zheng-Wen; Feng, Xiao-Yi; Peng, Jin-Ye; Zeng, Gui-Hua; Qi, Jin

    2017-04-01

    Based on chaos and quantum properties, we propose a quantum private communication scheme with dynamic control code sequence. The initial sequence is obtained via chaotic systems, and the control code sequence is derived by grouping, XOR and extracting. A shift cycle algorithm is designed to enable the dynamic change of control code sequence. Analysis shows that transmission efficiency could reach 100 % with high dynamics and security.

  15. Dynamics and control of tethered spacecraft during deployment and retrieval

    Science.gov (United States)

    Modi, V. J.; Lakshmanan, P. K.; Misra, A. K.

    The potential of tether-connected orbiting systems has led to numerous studies of their dynamics and control during deployment, operational (stationkeeeping), and retrieval phases. This paper examines some of the important aspects of the studies, including the modeling of tether dynamics and control, and system dynamics and control. Significant conclusions based on these studies are discussesd, and future research that would aid in a better understanding of the system performance is outlined.

  16. Dynamics of Cell Ensembles on Adhesive Micropatterns: Bridging the Gap between Single Cell Spreading and Collective Cell Migration.

    Directory of Open Access Journals (Sweden)

    Philipp J Albert

    2016-04-01

    Full Text Available The collective dynamics of multicellular systems arise from the interplay of a few fundamental elements: growth, division and apoptosis of single cells; their mechanical and adhesive interactions with neighboring cells and the extracellular matrix; and the tendency of polarized cells to move. Micropatterned substrates are increasingly used to dissect the relative roles of these fundamental processes and to control the resulting dynamics. Here we show that a unifying computational framework based on the cellular Potts model can describe the experimentally observed cell dynamics over all relevant length scales. For single cells, the model correctly predicts the statistical distribution of the orientation of the cell division axis as well as the final organisation of the two daughters on a large range of micropatterns, including those situations in which a stable configuration is not achieved and rotation ensues. Large ensembles migrating in heterogeneous environments form non-adhesive regions of inward-curved arcs like in epithelial bridge formation. Collective migration leads to swirl formation with variations in cell area as observed experimentally. In each case, we also use our model to predict cell dynamics on patterns that have not been studied before.

  17. Analysis of stochastic stem cell models with control.

    Science.gov (United States)

    Yang, Jienian; Sun, Zheng; Komarova, Natalia L

    2015-08-01

    Understanding the dynamics of stem cell lineages is of central importance both for healthy and cancerous tissues. We study stochastic population dynamics of stem cells and differentiated cells, where cell decisions, such as proliferation vs. differentiation decisions, or division and death decisions, are under regulation from surrounding cells. The goal is to understand how different types of control mechanisms affect the means and variances of cell numbers. We use the assumption of weak dependencies of the regulatory functions (the controls) on the cell populations near the equilibrium to formulate moment equations. We then study three different methods of closure, showing that they all lead to the same results for the highest order terms in the expressions for the moments. We derive simple explicit expressions for the means and the variances of stem cell and differentiated cell numbers. It turns out that the variance is expressed as an algebraic function of partial derivatives of the controls with respect to the population sizes at the equilibrium. We demonstrate that these findings are consistent with the results previously obtained in the context of particular systems, and also present two novel examples with negative and positive control of division and differentiation decisions. This methodology is formulated without any specific assumptions on the functional form of the controls, and thus can be used for any biological system.

  18. Dynamic metabolic flux analysis of plant cell wall synthesis.

    Science.gov (United States)

    Chen, Xuewen; Alonso, Ana P; Shachar-Hill, Yair

    2013-07-01

    The regulation of plant cell wall synthesis pathways remains poorly understood. This has become a bottleneck in designing bioenergy crops. The goal of this study was to analyze the regulation of plant cell wall precursor metabolism using metabolic flux analysis based on dynamic labeling experiments. Arabidopsis T87 cells were cultured heterotrophically with (13)C labeled sucrose. The time course of ¹³C labeling patterns in cell wall precursors and related sugar phosphates was monitored using liquid chromatography tandem mass spectrometry until steady state labeling was reached. A kinetic model based on mass action reaction mechanisms was developed to simulate the carbon flow in the cell wall synthesis network. The kinetic parameters of the model were determined by fitting the model to the labeling time course data, cell wall composition, and synthesis rates. A metabolic control analysis was performed to predict metabolic regulations that may improve plant biomass composition for biofuel production. Our results describe the routes and rates of carbon flow from sucrose to cell wall precursors. We found that sucrose invertase is responsible for the entry of sucrose into metabolism and UDP-glucose-4-epimerase plays a dominant role in UDP-Gal synthesis in heterotrophic Aradidopsis cells under aerobic conditions. We also predicted reactions that exert strong regulatory influence over carbon flow to cell wall synthesis and its composition.

  19. Dynamics of cell polarity in tissue morphogenesis: a comparative view from Drosophila and Ciona.

    Science.gov (United States)

    Veeman, Michael T; McDonald, Jocelyn A

    2016-01-01

    Tissues in developing embryos exhibit complex and dynamic rearrangements that shape forming organs, limbs, and body axes. Directed migration, mediolateral intercalation, lumen formation, and other rearrangements influence the topology and topography of developing tissues. These collective cell behaviors are distinct phenomena but all involve the fine-grained control of cell polarity. Here we review recent findings in the dynamics of polarized cell behavior in both the Drosophila ovarian border cells and the Ciona notochord. These studies reveal the remarkable reorganization of cell polarity during organ formation and underscore conserved mechanisms of developmental cell polarity including the Par/atypical protein kinase C (aPKC) and planar cell polarity pathways. These two very different model systems demonstrate important commonalities but also key differences in how cell polarity is controlled in tissue morphogenesis. Together, these systems raise important, broader questions on how the developmental control of cell polarity contributes to morphogenesis of diverse tissues across the metazoa.

  20. Toward Control of Universal Scaling in Critical Dynamics

    Science.gov (United States)

    2016-01-27

    to synergistically combine two powerful and very successful theories for non-linear stochastic dynamics of cooperative multi-component systems , namely...we have now defined various tractable theoretical model systems that will allow the external control of universal dynamical scaling features through...competition models in evolutionary game theory and population dynamics . His calculations specifically address the purported mapping of these systems

  1. Identification and stochastic control of helicopter dynamic modes

    Science.gov (United States)

    Molusis, J. A.; Bar-Shalom, Y.

    1983-01-01

    A general treatment of parameter identification and stochastic control for use on helicopter dynamic systems is presented. Rotor dynamic models, including specific applications to rotor blade flapping and the helicopter ground resonance problem are emphasized. Dynamic systems which are governed by periodic coefficients as well as constant coefficient models are addressed. The dynamic systems are modeled by linear state variable equations which are used in the identification and stochastic control formulation. The pure identification problem as well as the stochastic control problem which includes combined identification and control for dynamic systems is addressed. The stochastic control problem includes the effect of parameter uncertainty on the solution and the concept of learning and how this is affected by the control's duel effect. The identification formulation requires algorithms suitable for on line use and thus recursive identification algorithms are considered. The applications presented use the recursive extended kalman filter for parameter identification which has excellent convergence for systems without process noise.

  2. Mechanosensing Dynamics of Red blood Cells

    Science.gov (United States)

    Wan, Jiandi

    2015-11-01

    Mechanical stress-induced deformation of human red blood cells (RBCs) plays important physiopathological roles in oxygen delivery, blood rheology, transfusion, and malaria. Recent studies demonstrate that, in response to mechanical deformation, RBCs release adenosine-5'-triphosphate (ATP), suggesting the existence of mechanotransductive pathways in RBCs. Most importantly, the released ATP from RBCs regulates vascular tone and impaired release of ATP from RBCs has been linked to diseases such as type II diabetes and cystic fibrosis. To date, however, the mechanisms of mechanotransductive release of ATP from RBCs remain unclear. Given that RBCs experience shear stresses continuously during the circulation cycle and the released ATP plays a central role in vascular physiopathology, understanding the mechanotransductive release of ATP from RBCs will provide not only fundamental insights to the role of RBCs in vascular homeostasis but also novel therapeutic strategies for red cell dysfunction and vascular disease. This talk describes the main research in my group on integrating microfluidic-based approaches to study the mechanosensing dynamics of RBCs. Specifically, I will introduce a micro?uidic approach that can probe the dynamics of shear-induced ATP release from RBCs with millisecond resolution and provide quantitative understandings of the mechanosensitive ATP release processes in RBCs. Furthermore, I will also describe our recent findings about the roles of the Piezo1 channel, a newly discovered mechanosensitive cation channel in the mechanotransductive ATP release in RBCs. Last, possible functions of RBCs in the regulation of cerebral blood flow will be discussed.

  3. Sperm Cell Dynamics in Shallow Chambers

    Science.gov (United States)

    Condat, Carlos; Marconi, Veronica; Guidobaldi, Alejandro; Giojalas, Laura; Silhanek, Alejandro; Jeyaram, Yogesh; Moshchalkov, Victor

    2015-03-01

    Self-propelled microorganisms are attracted to surfaces. This makes their dynamic behavior in restricted geometries very different from that observed in the bulk. Here we analyze the motion of spermatozoids confined to shallow chambers, investigating the nature of the cell trajectories and their accumulation near the side boundaries. Observed cell trajectories are composed of a succession of quasi-circular and quasi-linear segments. This suggests that the cells follow a path of intermittent trappings near the top and down surfaces separated by stretches of quasi-free motion near the center of the gap. Use of microstructured petal-shaped edges limits accumulation near the borders and contributes to increase the concentration in the chamber interior. System stabilization occurs over times of the order of minutes, which agrees well with a theoretical estimate that assumes that the cell mean-square displacement is largely due to the quasi-linear segments. Pure quasi-circular trajectories would require several hours to stabilize. Our estimates also indicate that stabilization proceeds 2.5 times faster in the rosette geometries than in the smooth-edged chambers, which is another practical reason to prefer the former.

  4. New Highly Dynamic Approach for Thrust Vector Control

    Science.gov (United States)

    Hecht, M.; Ettl, J.; Grothe, D.; Hrbud, I.

    2015-09-01

    For a new launcher system a thrust vector control system is needed. This launch vehicle system consists of two rockets which are namely the VS-50 (two-stage suborbital vehicle) and the VLM-1 (three-stage microsatellite launch vehicle). VLM-1 and VS-50 are developed in a cooperation between the German Aerospace Center (DLR) and the Brazilian Aeronautics and Space Institute (IAE). To keep these two rockets on its trajectory during flight a highly dynamic thrust vector control system is required. For the purpose of developing such a highly dynamic thrust vector control system a master thesis was written by the author. The development includes all mechanical constructions as well as control algorithms and electronics design. Moreover an optimization of control algorithms was made to increase the dynamic capabilities of the thrust vector control system. The composition of the right components plus the sophisticated control algorithm make the thrust vector control system highly dynamic.

  5. Automated live cell imaging systems reveal dynamic cell behavior.

    Science.gov (United States)

    Chirieleison, Steven M; Bissell, Taylor A; Scelfo, Christopher C; Anderson, Jordan E; Li, Yong; Koebler, Doug J; Deasy, Bridget M

    2011-07-01

    Automated time-lapsed microscopy provides unique research opportunities to visualize cells and subcellular components in experiments with time-dependent parameters. As accessibility to these systems is increasing, we review here their use in cell science with a focus on stem cell research. Although the use of time-lapsed imaging to answer biological questions dates back nearly 150 years, only recently have the use of an environmentally controlled chamber and robotic stage controllers allowed for high-throughput continuous imaging over long periods at the cell and subcellular levels. Numerous automated imaging systems are now available from both companies that specialize in live cell imaging and from major microscope manufacturers. We discuss the key components of robots used for time-lapsed live microscopic imaging, and the unique data that can be obtained from image analysis. We show how automated features enhance experimentation by providing examples of uniquely quantified proliferation and migration live cell imaging data. In addition to providing an efficient system that drastically reduces man-hours and consumes fewer laboratory resources, this technology greatly enhances cell science by providing a unique dataset of temporal changes in cell activity. Copyright © 2011 American Institute of Chemical Engineers (AIChE).

  6. Improved generalized cell mapping for global analysis of dynamical systems

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Three main parts of generalized cell mapping are improved for global analysis. A simple method, which is not based on the theory of digraphs, is presented to locate complete self-cycling sets that corre- spond to attractors and unstable invariant sets involving saddle, unstable periodic orbit and chaotic saddle. Refinement for complete self-cycling sets is developed to locate attractors and unstable in- variant sets with high degree of accuracy, which can start with a coarse cell structure. A nonuniformly interior-and-boundary sampling technique is used to make the refinement robust. For homeomorphic dissipative dynamical systems, a controlled boundary sampling technique is presented to make gen- eralized cell mapping method with refinement extremely accurate to obtain invariant sets. Recursive laws of group absorption probability and expected absorption time are introduced into generalized cell mapping, and then an optimal order for quantitative analysis of transient cells is established, which leads to the minimal computational work. The improved method is applied to four examples to show its effectiveness in global analysis of dynamical systems.

  7. Improved generalized cell mapping for global analysis of dynamical systems

    Institute of Scientific and Technical Information of China (English)

    ZOU HaiLin; XU JianXue

    2009-01-01

    Three main parts of generalized cell mapping are improved for global analysis. A simple method, whichis not based on the theory of digraphs, is presented to locate complete self-cycling sets that corre-spond to attractors and unstable invariant sets involving saddle, unstable periodic orbit and chaotic saddle. Refinement for complete self-cycling sets is developed to locate attractors and unstable in-variant sets with high degree of accuracy, which can start with a coarse cell structure. A nonuniformly interior-and-boundary sampling technique is used to make the refinement robust. For homeomorphic dissipative dynamical systems, a controlled boundary sampling technique is presented to make gen-eralized cell mapping method with refinement extremely accurate to obtain invariant sets. Recursive laws of group absorption probability and expected absorption time are introduced into generalized cell mapping, and then an optimal order for quantitative analysis of transient cells is established, which leads to the minimal computational work. The improved method is applied to four examples to show its effectiveness in global analysis of dynamical systems.

  8. Model based control of dynamic atomic force microscope

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Chibum [Department of Mechanical System Design Engineering, Seoul National University of Science and Technology, Seoul 139-743 (Korea, Republic of); Salapaka, Srinivasa M., E-mail: salapaka@illinois.edu [Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801 (United States)

    2015-04-15

    A model-based robust control approach is proposed that significantly improves imaging bandwidth for the dynamic mode atomic force microscopy. A model for cantilever oscillation amplitude and phase dynamics is derived and used for the control design. In particular, the control design is based on a linearized model and robust H{sub ∞} control theory. This design yields a significant improvement when compared to the conventional proportional-integral designs and verified by experiments.

  9. Model based control of dynamic atomic force microscope.

    Science.gov (United States)

    Lee, Chibum; Salapaka, Srinivasa M

    2015-04-01

    A model-based robust control approach is proposed that significantly improves imaging bandwidth for the dynamic mode atomic force microscopy. A model for cantilever oscillation amplitude and phase dynamics is derived and used for the control design. In particular, the control design is based on a linearized model and robust H(∞) control theory. This design yields a significant improvement when compared to the conventional proportional-integral designs and verified by experiments.

  10. Adaptive Control of Robot Manipulators With Uncertain Kinematics and Dynamics

    OpenAIRE

    Wang, Hanlei

    2014-01-01

    In this paper, we investigate the adaptive control problem for robot manipulators with both the uncertain kinematics and dynamics. We propose two adaptive control schemes to realize the objective of task-space trajectory tracking irrespective of the uncertain kinematics and dynamics. The proposed controllers have the desirable separation property, and we also show that the first adaptive controller with appropriate modifications can yield improved performance, without the expense of conservat...

  11. Simulation and Experimental Investigation of Structural Dynamic Frequency Characteristics Control

    Directory of Open Access Journals (Sweden)

    Bing Li

    2012-04-01

    Full Text Available In general, mechanical equipment such as cars, airplanes, and machine tools all operate with constant frequency characteristics. These constant working characteristics should be controlled if the dynamic performance of the equipment demands improvement or the dynamic characteristics is intended to change with different working conditions. Active control is a stable and beneficial method for this, but current active control methods mainly focus on vibration control for reducing the vibration amplitudes in the time domain or frequency domain. In this paper, a new method of dynamic frequency characteristics active control (DFCAC is presented for a flat plate, which can not only accomplish vibration control but also arbitrarily change the dynamic characteristics of the equipment. The proposed DFCAC algorithm is based on a neural network including two parts of the identification implement and the controller. The effectiveness of the DFCAC method is verified by several simulation and experiments, which provide desirable results.

  12. Dynamics and Control of Humanoid Robots: A Geometrical Approach

    CERN Document Server

    Ivancevic, Vladimir G

    2011-01-01

    his paper reviews modern geometrical dynamics and control of humanoid robots. This general Lagrangian and Hamiltonian formalism starts with a proper definition of humanoid's configuration manifold, which is a set of all robot's active joint angles. Based on the `covariant force law', the general humanoid's dynamics and control are developed. Autonomous Lagrangian dynamics is formulated on the associated `humanoid velocity phase space', while autonomous Hamiltonian dynamics is formulated on the associated `humanoid momentum phase space'. Neural-like hierarchical humanoid control naturally follows this geometrical prescription. This purely rotational and autonomous dynamics and control is then generalized into the framework of modern non-autonomous biomechanics, defining the Hamiltonian fitness function. The paper concludes with several simulation examples. Keywords: Humanoid robots, Lagrangian and Hamiltonian formalisms, neural-like humanoid control, time-dependent biodynamics

  13. Human regulatory B cells control the TFH cell response.

    Science.gov (United States)

    Achour, Achouak; Simon, Quentin; Mohr, Audrey; Séité, Jean-François; Youinou, Pierre; Bendaoud, Boutahar; Ghedira, Ibtissem; Pers, Jacques-Olivier; Jamin, Christophe

    2017-07-01

    Follicular helper T (TFH) cells support terminal B-cell differentiation. Human regulatory B (Breg) cells modulate cellular responses, but their control of TFH cell-dependent humoral immune responses is unknown. We sought to assess the role of Breg cells on TFH cell development and function. Human T cells were polyclonally stimulated in the presence of IL-12 and IL-21 to generate TFH cells. They were cocultured with B cells to induce their terminal differentiation. Breg cells were included in these cultures, and their effects were evaluated by using flow cytometry and ELISA. B-cell lymphoma 6, IL-21, inducible costimulator, CXCR5, and programmed cell death protein 1 (PD-1) expressions increased on stimulated human T cells, characterizing TFH cell maturation. In cocultures they differentiated B cells into CD138(+) plasma and IgD(-)CD27(+) memory cells and triggered immunoglobulin secretions. Breg cells obtained by Toll-like receptor 9 and CD40 activation of B cells prevented TFH cell development. Added to TFH cell and B-cell cocultures, they inhibited B-cell differentiation, impeded immunoglobulin secretions, and expanded Foxp3(+)CXCR5(+)PD-1(+) follicular regulatory T cells. Breg cells modulated IL-21 receptor expressions on TFH cells and B cells, and their suppressive activities involved CD40, CD80, CD86, and intercellular adhesion molecule interactions and required production of IL-10 and TGF-β. Human Breg cells control TFH cell maturation, expand follicular regulatory T cells, and inhibit the TFH cell-mediated antibody secretion. These novel observations demonstrate a role for the Breg cell in germinal center reactions and suggest that deficient activities might impair the TFH cell-dependent control of humoral immunity and might lead to the development of aberrant autoimmune responses. Copyright © 2016 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  14. An Analytical Dynamics Approach to the Control of Mechanical Systems

    Science.gov (United States)

    Mylapilli, Harshavardhan

    A new and novel approach to the control of nonlinear mechanical systems is presented in this study. The approach is inspired by recent results in analytical dynamics that deal with the theory of constrained motion. The control requirements on the dynamical system are viewed from an analytical dynamics perspective and the theory of constrained motion is used to recast these control requirements as constraints on the dynamical system. Explicit closed form expressions for the generalized nonlinear control forces are obtained by using the fundamental equation of mechanics. The control so obtained is optimal at each instant of time and causes the constraints to be exactly satisfied. No linearizations and/or approximations of the nonlinear dynamical system are made, and no a priori structure is imposed on the nature of nonlinear controller. Three examples dealing with highly nonlinear complex dynamical systems that are chosen from diverse areas of discrete and continuum mechanics are presented to demonstrate the control approach. The first example deals with the energy control of underactuated inhomogeneous nonlinear lattices (or chains), the second example deals with the synchronization of the motion of multiple coupled slave gyros with that of a master gyro, and the final example deals with the control of incompressible hyperelastic rubber-like thin cantilever beams. Numerical simulations accompanying these examples show the ease, simplicity and the efficacy with which the control methodology can be applied and the accuracy with which the desired control objectives can be met.

  15. Dynamic cell culture system (7-IML-1)

    Science.gov (United States)

    Cogoli, Augusto

    1992-01-01

    This experiment is one of the Biorack experiments being flown on the International Microgravity Laboratory 1 (MIL-1) mission as part of an investigation studying cell proliferation and performance in space. One of the objectives of this investigation is to assess the potential benefits of bioprocessing in space with the ultimate goal of developing a bioreactor for continuous cell cultures in space. This experiment will test the operation of an automated culture chamber that was designed for use in a Bioreactor in space. The device to be tested is called the Dynamic Cell Culture System (DCCS). It is a simple device in which media are renewed or chemicals are injected automatically, by means of osmotic pumps. This experiment uses four Type I/O experiment containers. One DCCS unit, which contains a culture chamber with renewal of medium and a second chamber without a medium supply fits in each container. Two DCCS units are maintained under zero gravity conditions during the on-orbit period. The other two units are maintained under 1 gh conditions in a 1 g centrifuge. The schedule for incubator transfer is given.

  16. Dynamic contrast-enhanced MRI to predict response to vinorelbine-cisplatin alone or with rh-endostatin in patients with non-small cell lung cancer and bone metastases: a randomised, double-blind, placebo-controlled trial.

    Science.gov (United States)

    Zhang, Rui; Wang, Zhi-Yu; Li, Yue-Hua; Lu, Yao-Hong; Wang, Shuai; Yu, Wen-Xi; Zhao, Hui

    2016-10-01

    Metastatic bone disease is a frequent complication of advanced non-small-cell lung cancer and causes skeletal-related events which result in a poor prognosis. A standard method to assess the therapeutic response of bone metastases does not currently exist. We used dynamic contrast-enhanced MRI to obtain quantitative measures to assess the suitability of this technique to gauge therapeutic response to vinorelbine-cisplatin plus rh-endostatinfor previously untreated non-small cell lung cancer with bone metastases. We did a phase 4, randomised, prospective, double-blind, placebo-controlled clinical trial in Shanghai Sixth People's Hospital, Shanghai, China. Inclusion criteria were non-small-cell lung cancer patients with bone metastases confirmed by pathology or cytology; available imaging data of pelvic metastatic lesions; aged 18 to 75 years old; expected survival at least 3 months; not receiving taxane, bevacizumab, thalidomide, rh-endostatin, or bisphosphonate; not having radiation therapy within 3 months of enrollment into study; normal results of routine blood tests, liver and kidney function, and electrocardiogram; absence of cardiovascular disease, autoimmune disease, vasculitis, severe infection, diabetes, and other concomitant disease; and signed informed consent. Exclusion criteria were receiving granulocyte colony stimulating factor or granulocyte-macrophage colony stimulating factor during chemotherapy, intolerance to adverse reaction, and allergy to contrast agents. Patients were randomly assigned to treatment group and control group at a ratio of 2:1 by random code generation by an independent biostatistician in a double-blind fashion. Participants received either vinorelbine-cisplatin plus rh-endostatin or vinorelbine-cisplatin plus placebo. Vinorelbine (25 mg/m(2)) and cisplatin (75 mg/m(2)) were administered intravenously on the first day of a 21 day cycle. Patients received rh-endostatin (7·5 mg/m(2)) or placebo on days 1-14 of a cycle. The primary

  17. Modeling and control of fuel cell systems and fuel processors

    Science.gov (United States)

    Pukrushpan, Jay Tawee

    Fuel cell systems offer clean and efficient energy production and are currently under intensive development by several manufacturers for both stationary and mobile applications. The viability, efficiency, and robustness of this technology depend on understanding, predicting, and controlling the unique transient behavior of the fuel cell system. In this thesis, we employ phenomenological modeling and multivariable control techniques to provide fast and consistent system dynamic behavior. Moreover, a framework for analyzing and evaluating different control architectures and sensor sets is provided. Two fuel cell related control problems are investigated in this study, namely, the control of the cathode oxygen supply for a high-pressure direct hydrogen Fuel Cell System (FCS) and control of the anode hydrogen supply from a natural gas Fuel Processor System (FPS). System dynamic analysis and control design is carried out using model-based linear control approaches. A system level dynamic model suitable for each control problem is developed from physics-based component models. The transient behavior captured in the model includes flow characteristics, inertia dynamics, lumped-volume manifold filling dynamics, time evolving spatially-homogeneous reactant pressure or mole fraction, membrane humidity, and the Catalytic Partial Oxidation (CPOX) reactor temperature. The goal of the FCS control problem is to effectively regulate the oxygen concentration in the cathode by quickly and accurately replenishing oxygen depleted during power generation. The features and limitations of different control configurations and the effect of various measurement on the control performance are examined. For example, an observability analysis suggests using the stack voltage measurement as feedback to the observer-based controller to improve the closed loop performance. The objective of the FPS control system is to regulate both the CPOX temperature and anode hydrogen concentration. Linear

  18. Advances in analysis and control of timedelayed dynamical systems

    CERN Document Server

    Sun, Jianqiao

    2013-01-01

    Analysis and control of timedelayed systems have been applied in a wide range of applications, ranging from mechanical, control, economic, to biological systems. Over the years, there has been a steady stream of interest in timedelayed dynamic systems, this book takes a snap shot of recent research from the world leading experts in analysis and control of dynamic systems with time delay to provide a bird's eye view of its development. The topics covered in this book include solution methods, stability analysis and control of periodic dynamic systems with time delay, bifurcations, stochastic dy

  19. Dynamic range control of audio signals by digital signal processing

    Science.gov (United States)

    Gilchrist, N. H. C.

    It is often necessary to reduce the dynamic range of musical programs, particularly those comprising orchestral and choral music, for them to be received satisfactorily by listeners to conventional FM and AM broadcasts. With the arrival of DAB (Digital Audio Broadcasting) a much wider dynamic range will become available for radio broadcasting, although some listeners may prefer to have a signal with a reduced dynamic range. This report describes a digital processor developed by the BBC to control the dynamic range of musical programs in a manner similar to that of a trained Studio Manager. It may be used prior to transmission in conventional broadcasting, replacing limiters or other compression equipment. In DAB, it offers the possibility of providing a dynamic range control signal to be sent to the receiver via an ancillary data channel, simultaneously with the uncompressed audio, giving the listener the option of the full dynamic range or a reduced dynamic range.

  20. Coupled dynamic systems and Le Chatelier's principle in noise control

    Science.gov (United States)

    Maidanik, G.; Becker, K. J.

    2004-05-01

    Investigation of coupling an externally driven dynamic system-a master dynamic system-to a passive one-an adjunct dynamic system-reveals that the response of the adjunct dynamic system affects the precoupled response of the master dynamic system. The responses, in the two dynamic systems when coupled, are estimated by the stored energies (Es) and (E0), respectively. Since the adjunct dynamic system, prior to coupling, was with zero (0) stored energy, E0s=0, the precoupled stored energy (E00) in the master dynamic system is expected to be reduced to (E0) when coupling is instituted; i.e., one expects E0control of the master dynamic system would result from the coupling. It is argued that the change in the disposition of the stored energies as just described may not be the only change. The coupling may influence the external input power into the master dynamic system which may interfere with the expected noise control. Indeed, the coupling may influence the external input power such that the expected beneficial noise control may not materialize. Examples of these kinds of noise control reversals are cited.

  1. Optimal control of HIV/AIDS dynamic: Education and treatment

    Science.gov (United States)

    Sule, Amiru; Abdullah, Farah Aini

    2014-07-01

    A mathematical model which describes the transmission dynamics of HIV/AIDS is developed. The optimal control representing education and treatment for this model is explored. The existence of optimal Control is established analytically by the use of optimal control theory. Numerical simulations suggest that education and treatment for the infected has a positive impact on HIV/AIDS control.

  2. System level modeling and component level control of fuel cells

    Science.gov (United States)

    Xue, Xingjian

    This dissertation investigates the fuel cell systems and the related technologies in three aspects: (1) system-level dynamic modeling of both PEM fuel cell (PEMFC) and solid oxide fuel cell (SOFC); (2) condition monitoring scheme development of PEM fuel cell system using model-based statistical method; and (3) strategy and algorithm development of precision control with potential application in energy systems. The dissertation first presents a system level dynamic modeling strategy for PEM fuel cells. It is well known that water plays a critical role in PEM fuel cell operations. It makes the membrane function appropriately and improves the durability. The low temperature operating conditions, however, impose modeling difficulties in characterizing the liquid-vapor two phase change phenomenon, which becomes even more complex under dynamic operating conditions. This dissertation proposes an innovative method to characterize this phenomenon, and builds a comprehensive model for PEM fuel cell at the system level. The model features the complete characterization of multi-physics dynamic coupling effects with the inclusion of dynamic phase change. The model is validated using Ballard stack experimental result from open literature. The system behavior and the internal coupling effects are also investigated using this model under various operating conditions. Anode-supported tubular SOFC is also investigated in the dissertation. While the Nernst potential plays a central role in characterizing the electrochemical performance, the traditional Nernst equation may lead to incorrect analysis results under dynamic operating conditions due to the current reverse flow phenomenon. This dissertation presents a systematic study in this regard to incorporate a modified Nernst potential expression and the heat/mass transfer into the analysis. The model is used to investigate the limitations and optimal results of various operating conditions; it can also be utilized to perform the

  3. Output tracking control of mobile manipulators based on dynamical sliding-mode control

    Institute of Scientific and Technical Information of China (English)

    WU Yuxiang; FENG Ying; HU Yueming

    2007-01-01

    A dynamical sliding-mode controller is devised to track the output of mobile manipulators. During the investi- gation, firstly a reduced dynamic model considering the dynamics of the driving motor is developed for mobile manipulators. Then, the system is decomposed into four lower-dimensional subsystems by means of diffeomorphism and nonlinear input transformation. Moreover, a design method of the dynamical sliding-mode controller that is applied to the output tracking of mobile manipulators is proposed. The simulation results indicate that the dynamical sliding-mode controller can not only track the given trajec- tory correctly but also reduce the chattering of sliding-mode control system considerably.

  4. DYNAMICS BASED CONTROL OF A SKID STEERING MOBILE ROBOT

    Directory of Open Access Journals (Sweden)

    Osama Elshazly

    2016-06-01

    Full Text Available In this paper, development of a reduced order, augmented dynamics-drive model that combines both the dynamics and drive subsystems of the skid steering mobile robot (SSMR is presented. A Linear Quadratic Regulator (LQR control algorithm with feed-forward compensation of the disturbances part included in the reduced order augmented dynamics-drive model is designed. The proposed controller has many advantages such as its simplicity in terms of design and implementation in comparison with complex nonlinear control schemes that are usually designed for this system. Moreover, the good performance is also provided by the controller for the SSMR comparable with a nonlinear controller based on the inverse dynamics which depends on the availability of an accurate model describing the system. Simulation results illustrate the effectiveness and enhancement provided by the proposed controller.

  5. Dynamic Primitives in the Control of Locomotion

    Directory of Open Access Journals (Sweden)

    Neville eHogan

    2013-06-01

    Full Text Available Humans achieve locomotor dexterity that far exceeds the capability of modern robots, yet this is achieved despite slower actuators, imprecise sensors and vastly slower communication. We propose that this spectacular performance arises from encoding motor commands in terms of dynamic primitives. We propose three primitives as a foundation for a comprehensive theoretical framework that can embrace a wide range of upper- and lower-limb behaviors. Building on previous work that suggested discrete and rhythmic movements as elementary dynamic behaviors, we define submovements and oscillations: As discrete movements cannot be combined with sufficient flexibility, we argue that suitably-defined submovements are primitives. As the term rhythmic may be ambiguous, we define oscillations as the corresponding class of primitives. We further propose mechanical impedances as a third class of dynamic primitives, necessary for interaction with the physical environment. Combination of these three classes of primitive requires care. One approach is through a generalized equivalent network: a virtual trajectory composed of simultaneous and/or sequential submovements and/or oscillations that interacts with mechanical impedances to produce observable forces and motions. Reliable experimental identification of these dynamic primitives presents challenges: Identification of mechanical impedances is exquisitely sensitive to assumptions about their dynamic structure; identification of submovements and oscillations is sensitive to their assumed form and to details of the algorithm used to extract them. Some methods to address these challenges are presented. Some implications of this theoretical framework for locomotor rehabilitation are considered.

  6. Phase resetting reveals network dynamics underlying a bacterial cell cycle.

    Directory of Open Access Journals (Sweden)

    Yihan Lin

    Full Text Available Genomic and proteomic methods yield networks of biological regulatory interactions but do not provide direct insight into how those interactions are organized into functional modules, or how information flows from one module to another. In this work we introduce an approach that provides this complementary information and apply it to the bacterium Caulobacter crescentus, a paradigm for cell-cycle control. Operationally, we use an inducible promoter to express the essential transcriptional regulatory gene ctrA in a periodic, pulsed fashion. This chemical perturbation causes the population of cells to divide synchronously, and we use the resulting advance or delay of the division times of single cells to construct a phase resetting curve. We find that delay is strongly favored over advance. This finding is surprising since it does not follow from the temporal expression profile of CtrA and, in turn, simulations of existing network models. We propose a phenomenological model that suggests that the cell-cycle network comprises two distinct functional modules that oscillate autonomously and couple in a highly asymmetric fashion. These features collectively provide a new mechanism for tight temporal control of the cell cycle in C. crescentus. We discuss how the procedure can serve as the basis for a general approach for probing network dynamics, which we term chemical perturbation spectroscopy (CPS.

  7. Dynamic decoupling nonlinear control method for aircraft gust alleviation

    Science.gov (United States)

    Lv, Yang; Wan, Xiaopeng; Li, Aijun

    2008-10-01

    A dynamic decoupling nonlinear control method for MIMO system is presented in this paper. The dynamic inversion method is used to decouple the multivariable system. The nonlinear control method is used to overcome the poor decoupling effect when the system model is inaccurate. The nonlinear control method has correcting function and is expressed in analytic form, it is easy to adjust the parameters of the controller and optimize the design of the control system. The method is used to design vertical transition mode of active control aircraft for gust alleviation. Simulation results show that the designed vertical transition mode improves the gust alleviation effect about 34% comparing with the normal aircraft.

  8. MEMS-based dynamic cell-to-cell culture platforms using electrochemical surface modifications

    Science.gov (United States)

    Chang, Jiyoung; Yoon, Sang-Hee; Mofrad, Mohammad R. K.; Lin, Liwei

    2011-05-01

    MEMS-based biological platforms with the capability of both spatial placements and time releases of living cells for cell-to-cell culture experiments have been designed and demonstrated utilizing electrochemical surface modification effects. The spatial placement is accomplished by electrochemical surface modification of substrate surfaces to be either adhesive or non-adhesive for living cells. The time control is achieved by the electrical activation of the selective indium tin oxide co-culture electrode to allow the migration of living cells onto the electrode to start the cell-to-cell culture studies. Prototype devices have a three-electrode design with an electrode size of 50 × 50 µm2 and the separation gaps of 2 µm between them. An electrical voltage of -1.5 V has been used to activate the electrodes independently and sequentially to demonstrate the dynamic cell-to-cell culture experiments of NIH 3T3 fibroblast and Madin Darby canine kidney cells. As such, this MEMS platform could be a basic yet versatile tool to characterize transient cell-to-cell interactions.

  9. Beta cell dynamics: beta cell replenishment, beta cell compensation and diabetes.

    Science.gov (United States)

    Cerf, Marlon E

    2013-10-01

    Type 2 diabetes, characterized by persistent hyperglycemia, arises mostly from beta cell dysfunction and insulin resistance and remains a highly complex metabolic disease due to various stages in its pathogenesis. Glucose homeostasis is primarily regulated by insulin secretion from the beta cells in response to prevailing glycemia. Beta cell populations are dynamic as they respond to fluctuating insulin demand. Beta cell replenishment and death primarily regulate beta cell populations. Beta cells, pancreatic cells, and extra-pancreatic cells represent the three tiers for replenishing beta cells. In rodents, beta cell self-replenishment appears to be the dominant source for new beta cells supported by pancreatic cells (non-beta islet cells, acinar cells, and duct cells) and extra-pancreatic cells (liver, neural, and stem/progenitor cells). In humans, beta cell neogenesis from non-beta cells appears to be the dominant source of beta cell replenishment as limited beta cell self-replenishment occurs particularly in adulthood. Metabolic states of increased insulin demand trigger increased insulin synthesis and secretion from beta cells. Beta cells, therefore, adapt to support their physiology. Maintaining physiological beta cell populations is a strategy for targeting metabolic states of persistently increased insulin demand as in diabetes.

  10. Dynamic Intelligent Feedback Scheduling in Networked Control Systems

    Directory of Open Access Journals (Sweden)

    Hui-ying Chen

    2013-01-01

    Full Text Available For the networked control system with limited bandwidth and flexible workload, a dynamic intelligent feedback scheduling strategy is proposed. Firstly, a monitor is used to acquire the current available network bandwidth. Then, the new available bandwidth in the next interval is predicted by using LS_SVM approach. At the same time, the dynamic performance indices of all control loops are obtained with a two-dimensional fuzzy logic modulator. Finally, the predicted network bandwidth is dynamically allocated by the bandwidth manager and the priority allocator in terms of the loops' dynamic performance indices. Simulation results show that the sampling periods and priorities of control loops are adjusted timely according to the network workload condition and the dynamic performance of control loops, which make the system running in the optimal state all the time.

  11. Structure-based control of complex networks with nonlinear dynamics

    CERN Document Server

    Zañudo, Jorge G T; Albert, Réka

    2016-01-01

    Given the network of interactions underlying a complex system, what can we learn about controlling such a system solely from its structure? Over a century of research in control theory has given us tools to answer this question, which were widely applied in science and engineering. Yet the current tools do not always consider the inherently nonlinear dynamics of real systems and the naturally occurring system states in their definition of "control", a term whose interpretation varies across disciplines. Here we use a new mathematical framework for structure-based control of networks governed by a broad class of nonlinear dynamics that includes the major dynamic models of biological, technological, and social processes. This framework provides realizable node overrides that steer a system towards any of its natural long term dynamic behaviors and which are guaranteed to be effective regardless of the dynamic details and parameters of the underlying system. We use this framework on several real networks, compar...

  12. Dynamic inflow compensation for pitch controlled wind turbines

    Energy Technology Data Exchange (ETDEWEB)

    Van Engelen, T.G.; Van der Hooft, E.L. [ECN Wind Energy, Petten (Netherlands)

    2004-11-01

    An algorithm has been developed that provides filter parameters for pitch control loops in order to compensate for dynamic inflow effects. This improves the loop stability, especially near rated conditions. The filter behaviour reflects the 'inverse of the normalised' rotor-integral dynamic wake behaviour in accordance with the ECN differential equation wake model. Any (existing) pitch controller can be upgraded with such a filter. The algorithm has been implemented and incorporated in the ECN controlled design tool.

  13. Dynamic Teams and Decentralized Control Problems with Substitutable Actions

    OpenAIRE

    Asghari, Seyed Mohammad; Nayyar, Ashutosh

    2016-01-01

    This paper considers two problems -- a dynamic team problem and a decentralized control problem. The problems we consider do not belong to the known classes of "simpler" dynamic team/decentralized control problems such as partially nested or quadratically invariant problems. However, we show that our problems admit simple solutions under an assumption referred to as the substitutability assumption. Intuitively, substitutability in a team (resp. decentralized control) problem means that the ef...

  14. Dynamics and Control of a Class of Underactuated Mechanical Systems

    OpenAIRE

    Reyhanoglu, Mahmut; van der Schaft, Arjan; McClamroch, N. Harris; Kolmanovsky, Ilya

    1999-01-01

    This paper presents a theoretical framework for the dynamics and control of underactuated mechanical systems, defined as systems with fewer inputs than degrees of freedom. Control system formulation of underactuated mechanical systems is addressed and a class of underactuated systems characterized by nonintegrable dynamics relations is identified. Controllability and stabilizability results are derived for this class of underactuated systems. Examples are included to illustrate the results; t...

  15. Control of Exciton Dynamics in Nanodots for Quantum Operations

    Science.gov (United States)

    Chen, Pochung; Piermarocchi, C.; Sham, L. J.

    2001-08-01

    We present a theory to further a new perspective of proactive control of exciton dynamics in the quantum limit. Circularly polarized optical pulses in a semiconductor nanodot are used to control the dynamics of two interacting excitons of opposite polarizations. Shaping of femtosecond laser pulses keeps the quantum operation within the decoherence time. Computation of the fidelity of the operations and application to the complete solution of a minimal quantum computing algorithm demonstrate in theory the feasibility of quantum control.

  16. Approximate Dynamic Programming in Tracking Control of a Robotic Manipulator

    OpenAIRE

    Marcin Szuster; Piotr Gierlak

    2016-01-01

    This article focuses on the implementation of an approximate dynamic programming algorithm in the discrete tracking control system of the three-degrees of freedom Scorbot-ER 4pc robotic manipulator. The controlled system is included in an articulated robots group which uses rotary joints to access their work space. The main part of the control system is a dual heuristic dynamic programming algorithm that consists of two structures designed in the form of neural networks: an actor and a critic...

  17. Chaos control of chaotic dynamical systems using backstepping design

    Energy Technology Data Exchange (ETDEWEB)

    Yassen, M.T. [Mathematics Department, Faculty of Science, Mansoura University, Mansoura 35516 (Egypt)] e-mail: mtyassen@yahoo.com

    2006-01-01

    This work presents chaos control of chaotic dynamical systems by using backstepping design method. This technique is applied to achieve chaos control for each of the dynamical systems Lorenz, Chen and Lue systems. Based on Lyapunov stability theory, control laws are derived. We used the same technique to enable stabilization of chaotic motion to a steady state as well as tracking of any desired trajectory to be achieved in a systematic way. Numerical simulations are shown to verify the results.

  18. Discrete-Time Controllability for Feedback Quantum Dynamics

    CERN Document Server

    Albertini, Francesca

    2010-01-01

    Controllability properties for discrete-time, Markovian quantum dynamics are investigated. We find that, while in general the controlled system is not finite-time controllable, feedback control allows for arbitrary asymptotic state-to-state transitions. Under further assumption on the form of the measurement, we show that finite-time controllability can be achieved in a time that scales linearly with the dimension of the system, and we provide an iterative procedure to design the unitary control actions.

  19. The Dynamic Model of Allocation Control in Venture Capital

    Institute of Scientific and Technical Information of China (English)

    TIAN Zeng-rui

    2008-01-01

    The allocation of control and stock in venture capital is the key point of the venture capital project. This paper develops a dynamic model of control and stock and profoundly analyses how to allocate the control between the entrepreneur and the venture capitalist. The model reveals the relationship of control and stock's structure, the time and the degree of imparting the control to the entrepreneur or the venture capitalist, the condition of retracting the control and compensation accordingly.

  20. Controlling carrier dynamics at the nanoscale

    Science.gov (United States)

    Cánovas, Enrique; Bonn, Mischa

    2016-10-01

    This Special issue is motivated by the occasion of the International Conference on Charge Carrier Dynamics at the Nanoscale (CCDNano), held in Santiago de Compostela (Spain) in September 2015. As chairs for the CCDNano meeting, we aimed at bringing together experts from different scientific fields in order to trigger interdisciplinary discussions and collaborations; the ultimate goal of the conference was to serve as a platform to advance and help unifying methodologies and theories from different research sub-fields. We also aimed at a deeper understanding of charge dynamics to contribute to the development of improved or novel nanostructured devices. This special issue keeps that spirit, and intends to provide an overview of ongoing research efforts regarding charge carrier dynamics at the nanoscale.

  1. Molecular mechanisms controlling the cell cycle in embryonic stem cells.

    Science.gov (United States)

    Abdelalim, Essam M

    2013-12-01

    Embryonic stem (ES) cells are originated from the inner cell mass of a blastocyst stage embryo. They can proliferate indefinitely, maintain an undifferentiated state (self-renewal), and differentiate into any cell type (pluripotency). ES cells have an unusual cell cycle structure, consists mainly of S phase cells, a short G1 phase and absence of G1/S checkpoint. Cell division and cell cycle progression are controlled by mechanisms ensuring the accurate transmission of genetic information from generation to generation. Therefore, control of cell cycle is a complicated process, involving several signaling pathways. Although great progress has been made on the molecular mechanisms involved in the regulation of ES cell cycle, many regulatory mechanisms remain unknown. This review summarizes the current knowledge about the molecular mechanisms regulating the cell cycle of ES cells and describes the relationship existing between cell cycle progression and the self-renewal.

  2. Cell-cycle times and the tumour control probability.

    Science.gov (United States)

    Maler, Adrian; Lutscher, Frithjof

    2010-12-01

    Mechanistic dynamic cell population models for the tumour control probability (TCP) to date have used a simplistic representation of the cell cycle: either an exponential cell-cycle time distribution (Zaider & Minerbo, 2000, Tumour control probability: a formulation applicable to any temporal protocol of dose delivery. Phys. Med. Biol., 45, 279-293) or a two-compartment model (Dawson & Hillen, 2006, Derivation of the tumour control probability (TCP) from a cell cycle model. Comput. Math. Methods Med., 7, 121-142; Hillen, de Vries, Gong & Yurtseven, 2009, From cell population models to tumour control probability: including cell cycle effects. Acta Oncol. (submitted)). Neither of these simplifications captures realistic cell-cycle time distributions, which are rather narrowly peaked around the mean. We investigate how including such distributions affects predictions of the TCP. At first, we revisit the so-called 'active-quiescent' model that splits the cell cycle into two compartments and explore how an assumption of compartmental independence influences the predicted TCP. Then, we formulate a deterministic age-structured model and a corresponding branching process. We find that under realistic cell-cycle time distributions, lower treatment intensities are sufficient to obtain the same TCP as in the aforementioned models with simplified cell cycles, as long as the treatment is constant in time. For fractionated treatment, the situation reverses such that under realistic cell-cycle time distributions, the model requires more intense treatment to obtain the same TCP.

  3. The Quadrotor Dynamic Modeling and Indoor Target Tracking Control Method

    Directory of Open Access Journals (Sweden)

    Dewei Zhang

    2014-01-01

    Full Text Available A reliable nonlinear dynamic model of the quadrotor is presented. The nonlinear dynamic model includes actuator dynamic and aerodynamic effect. Since the rotors run near a constant hovering speed, the dynamic model is simplified at hovering operating point. Based on the simplified nonlinear dynamic model, the PID controllers with feedback linearization and feedforward control are proposed using the backstepping method. These controllers are used to control both the attitude and position of the quadrotor. A fully custom quadrotor is developed to verify the correctness of the dynamic model and control algorithms. The attitude of the quadrotor is measured by inertia measurement unit (IMU. The position of the quadrotor in a GPS-denied environment, especially indoor environment, is estimated from the downward camera and ultrasonic sensor measurements. The validity and effectiveness of the proposed dynamic model and control algorithms are demonstrated by experimental results. It is shown that the vehicle achieves robust vision-based hovering and moving target tracking control.

  4. Dynamic imaging of cell-free and cell-associated viral capture in mature dendritic cells.

    Science.gov (United States)

    Izquierdo-Useros, Nuria; Esteban, Olga; Rodriguez-Plata, Maria T; Erkizia, Itziar; Prado, Julia G; Blanco, Julià; García-Parajo, Maria F; Martinez-Picado, Javier

    2011-12-01

    Dendritic cells (DCs) capture human immunodeficiency virus (HIV) through a non-fusogenic mechanism that enables viral transmission to CD4(+) T cells, contributing to in vivo viral dissemination. Although previous studies have provided important clues to cell-free viral capture by mature DCs (mDCs), dynamic and kinetic insight on this process is still missing. Here, we used three-dimensional video microscopy and single-particle tracking approaches to dynamically dissect both cell-free and cell-associated viral capture by living mDCs. We show that cell-free virus capture by mDCs operates through three sequential phases: virus binding through specific determinants expressed in the viral particle, polarized or directional movements toward concrete regions of the cell membrane and virus accumulation in a sac-like structure where trapped viral particles display a hindered diffusive behavior. Moreover, real-time imaging of cell-associated viral transfer to mDCs showed a similar dynamics to that exhibited by cell-free virus endocytosis leading to viral accumulation in compartments. However, cell-associated HIV type 1 transfer to mDCs was the most effective pathway, boosted throughout enhanced cellular contacts with infected CD4(+) T cells. Our results suggest that in lymphoid tissues, mDC viral uptake could occur either by encountering cell-free or cell-associated virus produced by infected cells generating the perfect scenario to promote HIV pathogenesis and impact disease progression.

  5. Mitochondrial dynamics and the cell cycle

    Directory of Open Access Journals (Sweden)

    Penny M.A. Kianian

    2014-05-01

    Full Text Available Nuclear-mitochondrial (NM communication impacts many aspects of plant development including vigor, sterility and viability. Dynamic changes in mitochondrial number, shape, size, and cellular location takes place during the cell cycle possibly impacting the process itself and leading to distribution of this organelle into daughter cells. The genes that underlie these changes are beginning to be identified in model plants such as Arabidopsis. In animals disruption of the drp1 gene, a homolog to the plant drp3A and drp3B, delays mitochondrial division. This mutation results in increased aneuploidy due to chromosome mis-segregation. It remains to be discovered if a similar outcome is observed in plants. Alloplasmic lines provide an opportunity to understand the communication between the cytoplasmic organelles and the nucleus. Examples of studies in these lines, especially from the extensive collection in wheat, point to the role of mitochondria in chromosome movement, pollen fertility and other aspects of development. Genes involved in NM interaction also are believed to play a critical role in evolution of species and interspecific cross incompatibilities.

  6. Environmental and physiological control of dynamic photosynthesis

    NARCIS (Netherlands)

    Kaiser, M.E.

    2016-01-01

    Irradiance is the main driver of photosynthesis. In natural conditions, irradiance incident on a leaf often fluctuates, due to the movement of leaves, clouds and the sun. These fluctuations force photosynthesis to respond dynamically, however with delays that are subject to rate constants of underly

  7. System and Method for Dynamic Aeroelastic Control

    Science.gov (United States)

    Suh, Peter M. (Inventor)

    2015-01-01

    The present invention proposes a hardware and software architecture for dynamic modal structural monitoring that uses a robust modal filter to monitor a potentially very large-scale array of sensors in real time, and tolerant of asymmetric sensor noise and sensor failures, to achieve aircraft performance optimization such as minimizing aircraft flutter, drag and maximizing fuel efficiency.

  8. Dynamic formation control for autonomous underwater vehicles

    Institute of Scientific and Technical Information of China (English)

    燕雪峰; 古锋; 宋琛; 胡晓琳; 潘毅

    2014-01-01

    Path planning and formation structure forming are two of the most important problems for autonomous underwater vehicles (AUVs) to collaborate with each other. In this work, a dynamic formation model was proposed, in which several algorithms were developed for the complex underwater environment. Dimension changeable particle swarm algorithm was used to find an optimized path by dynamically adjusting the number and the distribution of the path nodes. Position relationship based obstacle avoidance algorithm was designed to detour along the edges of obstacles. Virtual potential point based formation-keeping algorithm was employed by incorporating dynamic strategies which were decided by the current states of the formation. The virtual potential point was used to keep the formation structure when the AUV or the formation was deviated. Simulation results show that an optimal path can be dynamically planned with fewer path nodes and smaller fitness, even with a concave obstacle. It has been also proven that different formation-keeping strategies can be adaptively selected and the formation can change its structure in a narrow area and restore back after passing the obstacle.

  9. Sliding-Mode Control of PEM Fuel Cells

    CERN Document Server

    Kunusch, Cristian; Mayosky, Miguel

    2012-01-01

    Recent advances in catalysis technologies and new materials make fuel cells an economically appealing and clean energy source with massive market potential in portable devices, home power generation and the automotive industry. Among the more promising fuel-cell technologies are proton exchange membrane fuel cells (PEMFCs). Sliding-Mode Control of PEM Fuel Cells demonstrates the application of higher-order sliding-mode control to PEMFC dynamics. Fuel-cell dynamics are often highly nonlinear and the text shows the advantages of sliding modes in terms of robustness to external disturbance, modelling error and system-parametric disturbance using higher-order control to reduce chattering. Divided into two parts, the book first introduces the theory of fuel cells and sliding-mode control. It begins by contextualising PEMFCs both in terms of their development and within the hydrogen economy and today’s energy production situation as a whole. The reader is then guided through a discussion of fuel-cell operation pr...

  10. Dynamic Control of a Space Robot System With No Thrust Jets Controlled Base

    Science.gov (United States)

    1991-08-01

    properties of the system are studied. It is found that the free-flying space robot dynamics cannot be linearly expressed in terms of dynamic parameters...control. Nearly all of these algorithms are based on the property of linwar parameterization in the robot dynamics , i.e., possibility of selecting a...proper set of equivalent parameters such that the robot dynamics depends linearly on these parameters. These controllers can take full consideration

  11. DRACULA: Dynamic range control for broadcasting and other applications

    Science.gov (United States)

    Gilchrist, N. H. C.

    The BBC has developed a digital processor which is capable of reducing the dynamic range of audio in an unobtrusive manner. It is ideally suited to the task of controlling the level of musical programs. Operating as a self-contained dynamic range controller, the processor is suitable for controlling levels in conventional AM or FM broadcasting, or for applications such as the compression of program material for in-flight entertainment. It can, alternatively, be used to provide a supplementary signal in DAB (digital audio broadcasting) for optional dynamic compression in the receiver.

  12. Control of stage by stage changing linear dynamic systems

    Directory of Open Access Journals (Sweden)

    Barseghyan V.R.

    2012-01-01

    Full Text Available In this paper, the control problems of linear dynamic systems stage by stage changing and the optimal control with the criteria of quality set for the whole range of time intervals are considered. The necessary and sufficient conditions of total controllability are also stated. The constructive solving method of a control problem is offered, as well as the definitions of conditions for the existence of programmed control and motions. The explicit form of control action for a control problem is constructed. The method for solving optimal control problem is offered, and the solution of optimal control of a specific target is brought.

  13. Dynamic analysis and control of novel moving mass flight vehicle

    Science.gov (United States)

    Li, Jianqing; Gao, Changsheng; Jing, Wuxing; Wei, Pengxin

    2017-02-01

    In terms of the moving mass control technology, the configuration of internal moving masses is a key challenge. In order to reduce the complexity of configuring these moving masses in a flight vehicle, a combination bank-to-turn control mode with the single moving mass and reaction jet is proposed in this paper. To investigate the dynamics and the potential of the control mechanism, an attitude dynamic model with single moving mass is generated. The dynamic analysis indicates that the control stability, control authority and dynamic behavior of the pitch channel are determined by the mass ratio of the moving mass to the system and the difference between the mass center of the moving mass and the mass center of the vehicle body. Interestingly, control authority increases proportionally with increasing mass ratio and also with decreasing the magnitude of the static margin. To deal with the coupling caused by the additional inertia moment which is generated by the motion of the moving mass, an adaptive control law by using dynamic inversion theory and the extended state observer is designed. Also, a compensator is designed for eliminating the influence of the servo actuator's dynamics on attitude of the flight vehicle. Finally, the simulation results validate the quality of the proposed adaptive controller which ensures a good performance in the novel configuration with internal moving mass.

  14. Active Control of Solar Array Dynamics During Spacecraft Maneuvers

    Science.gov (United States)

    Ross, Brant A.; Woo, Nelson; Kraft, Thomas G.; Blandino, Joseph R.

    2016-01-01

    Recent NASA mission plans require spacecraft to undergo potentially significant maneuvers (or dynamic loading events) with large solar arrays deployed. Therefore there is an increased need to understand and possibly control the nonlinear dynamics in the spacecraft system during such maneuvers. The development of a nonlinear controller is described. The utility of using a nonlinear controller to reduce forces and motion in a solar array wing during a loading event is demonstrated. The result is dramatic reductions in system forces and motion during a 10 second loading event. A motion curve derived from the simulation with the closed loop controller is used to obtain similar benefits with a simpler motion control approach.

  15. Fuzzy Logic Based Control of Power of PEM Fuel Cell System for Residential Application

    Directory of Open Access Journals (Sweden)

    Khaled MAMMAR

    2009-07-01

    Full Text Available This paper presents a dynamic model of Fuel cell system for residential power generation. The models proposed include a fuel cell stack model, reformer model and DC/AC inverter model. Furthermore a fuzzy logic (FLC controller is used to control active power of PEM fuel cell system. The controller modifies the hydrogen flow feedback from the terminal load. Simulation results confirmed the high performance capability of the fuzzy logic controller to control power generation.

  16. Fuzzy Logic Based Control of Power of PEM Fuel Cell System for Residential Application

    OpenAIRE

    Khaled MAMMAR; CHAKER, Abdelkader

    2009-01-01

    This paper presents a dynamic model of Fuel cell system for residential power generation. The models proposed include a fuel cell stack model, reformer model and DC/AC inverter model. Furthermore a fuzzy logic (FLC) controller is used to control active power of PEM fuel cell system. The controller modifies the hydrogen flow feedback from the terminal load. Simulation results confirmed the high performance capability of the fuzzy logic controller to control power generation.

  17. Fuel cell with internal flow control

    Science.gov (United States)

    Haltiner, Jr., Karl J.; Venkiteswaran, Arun [Karnataka, IN

    2012-06-12

    A fuel cell stack is provided with a plurality of fuel cell cassettes where each fuel cell cassette has a fuel cell with an anode and cathode. The fuel cell stack includes an anode supply chimney for supplying fuel to the anode of each fuel cell cassette, an anode return chimney for removing anode exhaust from the anode of each fuel cell cassette, a cathode supply chimney for supplying oxidant to the cathode of each fuel cell cassette, and a cathode return chimney for removing cathode exhaust from the cathode of each fuel cell cassette. A first fuel cell cassette includes a flow control member disposed between the anode supply chimney and the anode return chimney or between the cathode supply chimney and the cathode return chimney such that the flow control member provides a flow restriction different from at least one other fuel cell cassettes.

  18. Dynamic aspects of segmented mirror position control

    Science.gov (United States)

    Kaercher, Hans J.

    2006-02-01

    Extreme large optical telescopes will operate in an open environment and may be excited by wind effects. The position control of the mirror segments may need fast control, and the position actuators and the related control loops may be separated in a conventional slow, iso-static and a fast reaction-mass type system. There exists some experience with wind excitations of airborne telescopes, e.g. the Stratospheric Observatory for Infrared Astronomy SOFIA. The pointing control system of that telescope is equipped with several dedicated design features, as a vibration isolations system, a flexible body control system and an active mass damper system to handle excitations in different frequency ranges. These features may be transferred to the position control systems of segmented mirrors. The paper will give some system engi-neers recommendations for designing those systems.

  19. Environmental and physiological control of dynamic photosynthesis

    OpenAIRE

    Kaiser, M. E.

    2016-01-01

    Irradiance is the main driver of photosynthesis. In natural conditions, irradiance incident on a leaf often fluctuates, due to the movement of leaves, clouds and the sun. These fluctuations force photosynthesis to respond dynamically, however with delays that are subject to rate constants of underlying processes, such as regulation of electron transport, activation states of enzymes in the Calvin cycle, and stomatal conductance (gs). For example, in leaves adapted to low irradiance that are s...

  20. Nonlinear Dynamics and Control of Flexible Structures

    Science.gov (United States)

    1991-03-01

    Freedom," Ph.D. Thesis, Department of Theoretical and Applied Mechanics, Cornell University, in preparation. 5I I URI Reorts Islam , Saiful and Mircea...Theoretical and Applied Mechanics I S. Islam Civil and Environmental Engineering I 2! I 3 URI Accomplishments 3 -Nonlinear Dynamics and Chaos in Flexible...Structures with Symmetry," 31 (1991) 265-285. Islam , S. and M. Grigoriu, "Nonlinear Random Vibration of Pin-Jointed Trusses with Imperfections," in

  1. Unmodeled Dynamics in Robust Nonlinear Control

    Science.gov (United States)

    2000-08-01

    IEEE Transactions on Automatic Control , vol. 44, pp. 1975–1981, 1999. [6] D. Bestle...systems,” IEEE Transactions on Automatic Control , vol. 41, pp. 876–880, 1996. 95 [9] C.I. Byrnes and A. Isidori, “New results and examples in...Output-feedback stochastic nonlinear stabilization,” IEEE Transactions on Automatic Control , vol. 44, pp. 328–333, 1999. [14] J. Eker and K.J.

  2. DYNAMICAL VARIABLE STRUCTURE CONTROL OF A HELICOPTER IN VERTICAL FLIGHT

    OpenAIRE

    Sira-Ramirez, Hebertt; Zribi, Mohamed; Ahmad, Shaheen

    1991-01-01

    In this article, a dynamical multivariable discontinuous feedback control strategy of the sliding nlode type is proposed for the altitude stabilization of a nonlinear helicopter model in vertical flight. Vlrhile retaining the basic robustness features associated to sliding mode control policies, the proposed approach also results in smoothed out (i.e., non-chattering) input trajectories and controlled state variable responses.

  3. Dynamic stabilization methods for bilateral control of remote manipulation

    Science.gov (United States)

    Handlykken, M.

    1982-01-01

    This paper discusses and analyses several control strategies for generalized, bilateral master/slave manipulator systems. In these manipulators the two arms have different dynamic/kinematic properties which implies a more difficult control problem. Attention is focused on torque/force driven arms. Required performance for the necessary control computer(s) is discussed.

  4. Dynamics and Control of Adaptive Shells with Curvature Transformations

    OpenAIRE

    1995-01-01

    Adaptive structures with controllable geometries and shapes are rather useful in many engineering applications, such as adaptive wings, variable focus mirrors, adaptive machines, micro-electromechanical systems, etc. Dynamics and feedback control effectiveness of adaptive shells whose curvatures are actively controlled and continuously changed are evaluated. An adaptive piezoelectric laminated cylindrical shell composite with continuous curvature changes is studied, and its natural frequencie...

  5. Dynamical Epidemic Suppression Using Stochastic Prediction and Control

    Science.gov (United States)

    2004-10-28

    reduce the rate of input of susceptibles. By using the PDF flux, we are able to distinguish regions used in other chaos control schemes that are...use this information in a control algo- stochastic chaos control methods that account specifically for rithm to prevent bursting dynamics (that is, to

  6. Lipid signalling dynamics at the β-cell plasma membrane.

    Science.gov (United States)

    Wuttke, Anne

    2015-04-01

    Pancreatic β-cells are clustered in islets of Langerhans and secrete insulin in response to increased concentrations of circulating glucose. Insulin in turn acts on liver, muscle and fat tissue to store energy and normalize the blood glucose level. Inappropriate insulin release may lead to impaired glucose tolerance and diabetes. In addition to glucose, other nutrients, neural stimuli and hormonal stimuli control insulin secretion. Many of these signals are perceived at the plasma membrane, which is also the site where insulin granules undergo exocytosis. Therefore, it is not surprising that membrane lipids play an important role in the regulation of insulin secretion. β-cells release insulin in a pulsatile fashion. Signalling lipids integrate the nutrient and neurohormonal inputs to fine-tune, shape and co-ordinate the pulsatility. An important group of signalling lipids are phosphoinositides and their downstream messengers. This MiniReview will discuss new insights into lipid signalling dynamics in β-cells obtained from live-cell imaging experiments with fluorescent translocation biosensors. The plasma membrane concentration of several phosphoinositides and of their downstream messengers changes rapidly upon nutrient or neurohormonal stimulation. Glucose induces the most complex spatio-temporal patterns, typically involving oscillations of messenger concentrations, which sometimes are locally restricted. The tightly controlled levels of lipid messengers can mediate specific binding of downstream effectors to the plasma membrane, contributing to the appropriate regulation of insulin secretion.

  7. Satellite Dynamic Damping via Active Force Control Augmentation

    Science.gov (United States)

    Varatharajoo, Renuganth

    2012-07-01

    An approach that incorporates the Active Force Control (AFC) technique into a conventional Proportional-Derivative (PD) controller is proposed for a satellite active dynamic damping towards a full attitude control. The AFC method has been established to facilitate a robust motion control of dynamical systems in the presence of disturbances, parametric uncertainties and changes that are commonly prevalent in the real-world environment. The usefulness of the method can be extended by introducing intelligent mechanisms to approximate the mass or inertia matrix of the dynamic system to trigger the compensation effect of the controller. AFC is a technique that relies on the appropriate estimation of the inertial or mass parameters of the dynamic system and the measurements of the acceleration and force signals induced by the system if practical implementation is ever considered. In AFC, it is shown that the system subjected to a number of disturbances remains stable and robust via the compensating action of the control strategy. We demonstrate that it is possible to design a spacecraft attitude feedback controller that will ensure the system dynamics set point remains unchanged even in the presence of the disturbances provided that the actual disturbances can be modeled effectively. In order to further facilitate this analysis, a combined energy and attitude control system (CEACS) is proposed as a model satellite attitude control actuator. All the governing equations are established and the proposed satellite attitude control architecture is made amenable to numerical treatments. The results show that the PD-AFC attitude damping performances are superiorly better than that of the solely PD type. It is also shown that the tunings of the AFC system gains are crucial to ensure a better attitude damping performance and this process is mandatory for AFC systems. Finally, the results demonstrate an important satellite dynamic damping enhancement capability using the AFC

  8. Incremental approximate dynamic programming for nonlinear flight control design

    NARCIS (Netherlands)

    Zhou, Y.; Van Kampen, E.J.; Chu, Q.P.

    2015-01-01

    A self-learning adaptive flight control design for non-linear systems allows reliable and effective operation of flight vehicles in a dynamic environment. Approximate dynamic programming (ADP) provides a model-free and computationally effective process for designing adaptive linear optimal

  9. Dynamic Control of Completely Free-Flying Space Robot System

    Energy Technology Data Exchange (ETDEWEB)

    Lee, J.J. [Korea Advanced Energy Research Inst., Daeduk-Danji (Korea, Republic of). Korea Nuclear Safety Center; Xu, Y.; Kanade, T. [Carnegie-Mellon Univ., Pittsburgh, PA (United States). Robotics Inst.

    1993-03-01

    In this paper we discuss dynamic control of a completely- free-flying space robot system where the base attitude is not controlled. We first derive the system dynamic formulations in joint space and in inertia space, based on Lagrangian dynamics and linear and angular momentum conservation laws. The properties of completely free-flying robot system dynamics are studied. The nonlinear parameterization, one of the most important properties of the system dynamics, is demonstrated in theory and by a case study. Based on the system dynamic model in inertial space, globally stable dynamics control schemes are then proposed. Two algorithms are presented for the normal regulation problem and trajectory tracking applications. The PD algorithm is simple and easy to implement. The dynamic control algorithm has a fast and accurate system response even for the system with small mass/inertia ratio of the base with respect to the robot. The effectiveness of proposed algorithms is demonstrated by simulation studies. Future research work is identified. (author). 22 refs., 10 figs.

  10. Importance of Dynamic Inflow Model Predictive Control of Wind Turbines

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Knudsen, Torben; Overgaard, Anders;

    2015-01-01

    The efficiency of including dynamic inflow in the model based design of wind turbine controller has been discussed for many years in the wind energy community with out getting to a safe conclusion. This paper delivers a good argument in favor of including dynamic inflow. The main contributions...

  11. Improving the Dynamics of Suspension Bridges using Active Control Systems

    DEFF Research Database (Denmark)

    Thoft-Christensen, Palle

    Improving the dynamics of suspension bridge using active control is discussed in this paper. The main dynamic problem with long suspension bridges is the aeroelastic phenomenon called flutter. Flutter oscillations of a bridge girder is a stability problem and the oscillations are perpendicular...

  12. Kinematics and Dynamics of Motion Control Based on Acceleration Control

    Science.gov (United States)

    Ohishi, Kiyoshi; Ohba, Yuzuru; Katsura, Seiichiro

    The first IEEE International Workshop on Advanced Motion Control was held in 1990 pointed out the importance of physical interpretation of motion control. The software servoing technology is now common in machine tools, robotics, and mechatronics. It has been intensively developed for the numerical control (NC) machines. Recently, motion control in unknown environment will be more and more important. Conventional motion control is not always suitable due to the lack of adaptive capability to the environment. A more sophisticated ability in motion control is necessary for compliant contact with environment. Acceleration control is the key technology of motion control in unknown environment. The acceleration control can make a motion system to be a zero control stiffness system without losing the robustness. Furthermore, a realization of multi-degree-of-freedom motion is necessary for future human assistance. A human assistant motion will require various control stiffness corresponding to the task. The review paper focuses on the modal coordinate system to integrate the various control stiffness in the virtual axes. A bilateral teleoperation is a good candidate to consider the future human assistant motion and integration of decentralized systems. Thus the paper reviews and discusses the bilateral teleoperation from the control stiffness and the modal control design points of view.

  13. Dynamics systems vs. optimal control--a unifying view.

    Science.gov (United States)

    Schaal, Stefan; Mohajerian, Peyman; Ijspeert, Auke

    2007-01-01

    In the past, computational motor control has been approached from at least two major frameworks: the dynamic systems approach and the viewpoint of optimal control. The dynamic system approach emphasizes motor control as a process of self-organization between an animal and its environment. Nonlinear differential equations that can model entrainment and synchronization behavior are among the most favorable tools of dynamic systems modelers. In contrast, optimal control approaches view motor control as the evolutionary or development result of a nervous system that tries to optimize rather general organizational principles, e.g., energy consumption or accurate task achievement. Optimal control theory is usually employed to develop appropriate theories. Interestingly, there is rather little interaction between dynamic systems and optimal control modelers as the two approaches follow rather different philosophies and are often viewed as diametrically opposing. In this paper, we develop a computational approach to motor control that offers a unifying modeling framework for both dynamic systems and optimal control approaches. In discussions of several behavioral experiments and some theoretical and robotics studies, we demonstrate how our computational ideas allow both the representation of self-organizing processes and the optimization of movement based on reward criteria. Our modeling framework is rather simple and general, and opens opportunities to revisit many previous modeling results from this novel unifying view.

  14. A summary of the Dynamics Explorer /DE/-2 spacecraft attitude control operations and dynamics

    Science.gov (United States)

    Stengle, T. H.

    1982-01-01

    A summary of attitude control operations and observed attitude dynamics for the Dynamics Explorer (DE)-2 spacecraft is presented. By performing a systematic analysis of spacecraft drift and through optimization of modeling parameters in dynamics simulators, insight is given into spacecraft dynamics, techniques for reducing drift, and methods for streamlining operational procedures. This paper discusses how attitude and momentum drift were reduced for DE-2 by changing spacecraft geometry, altering operational procedures and making timely use of the control modes available. Attempts to correlate spacecraft drift activity with known environmental variables are made with only limited success.

  15. Nonlinear Dynamics and Control of Flexible Structures

    Science.gov (United States)

    1990-10-10

    control problem is to drive the outputs asymptotically to zero. Since output regulation problem seeks to enforce the set of constraints I hi() = , i = 1...K an m x m constant matrix, solves the output regulation problem if sliding can be achieved. In sliding the equivalent control is, Uq = -B(x)-KAz - B

  16. Motor Control Research Requires Nonlinear Dynamics

    Science.gov (United States)

    Guastello, Stephen J.

    2006-01-01

    The author comments on the original article "The Cinderella of psychology: The neglect of motor control in the science of mental life and behavior," by D. A. Rosenbaum. Rosenbaum draws attention to the study of motor control and evaluates seven possible explanations for why the topic has been relatively neglected. The point of this comment is that…

  17. Motor Control Research Requires Nonlinear Dynamics

    Science.gov (United States)

    Guastello, Stephen J.

    2006-01-01

    The author comments on the original article "The Cinderella of psychology: The neglect of motor control in the science of mental life and behavior," by D. A. Rosenbaum. Rosenbaum draws attention to the study of motor control and evaluates seven possible explanations for why the topic has been relatively neglected. The point of this comment is that…

  18. Model-based control of fuel cells (2): Optimal efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Golbert, Joshua; Lewin, Daniel R. [PSE Research Group, Wolfson Department of Chemical Engineering, Technion IIT, Haifa 32000 (Israel)

    2007-11-08

    A dynamic PEM fuel cell model has been developed, taking into account spatial dependencies of voltage, current, material flows, and temperatures. The voltage, current, and therefore, the efficiency are dependent on the temperature and other variables, which can be optimized on the fly to achieve optimal efficiency. In this paper, we demonstrate that a model predictive controller, relying on a reduced-order approximation of the dynamic PEM fuel cell model can satisfy setpoint changes in the power demand, while at the same time, minimize fuel consumption to maximize the efficiency. The main conclusion of the paper is that by appropriate formulation of the objective function, reliable optimization of the performance of a PEM fuel cell can be performed in which the main tunable parameter is the prediction and control horizons, V and U, respectively. We have demonstrated that increased fuel efficiency can be obtained at the expense of slower responses, by increasing the values of these parameters. (author)

  19. Fluid dynamics and noise in bacterial cell-cell and cell-surface scattering

    CERN Document Server

    Drescher, Knut; Cisneros, Luis H; Ganguly, Sujoy; Goldstein, Raymond E; 10.1073/pnas.1019079108

    2011-01-01

    Bacterial processes ranging from gene expression to motility and biofilm formation are constantly challenged by internal and external noise. While the importance of stochastic fluctuations has been appreciated for chemotaxis, it is currently believed that deterministic long-range fluid dynamical effects govern cell-cell and cell-surface scattering - the elementary events that lead to swarming and collective swimming in active suspensions and to the formation of biofilms. Here, we report the first direct measurements of the bacterial flow field generated by individual swimming Escherichia coli both far from and near to a solid surface. These experiments allowed us to examine the relative importance of fluid dynamics and rotational diffusion for bacteria. For cell-cell interactions it is shown that thermal and intrinsic stochasticity drown the effects of long-range fluid dynamics, implying that physical interactions between bacteria are determined by steric collisions and near-field lubrication forces. This dom...

  20. Chattering-Free Sliding Mode Control with Unmodeled Dynamics

    Science.gov (United States)

    Krupp, Don; Shtessel, Yuri B.

    1999-01-01

    Sliding mode control systems are valued for their robust accommodation of uncertainties and their ability to reject disturbances. In this paper, a design methodology is proposed to eliminate the chattering phenomenon affecting sliding mode controlled plants with input unmodeled actuator dynamics of second order or greater. The proposed controller design is based on the relative degrees of the plant and the unmodeled actuator dynamics and the ranges of the uncertainties of the plant and actuator. The controller utilizes the pass filter characteristics of the physical actuating device to provide a smoothing effect on the discontinuous control signal rather than introducing any artificial dynamics into the controller design thus eliminating chattering in the system's output response.

  1. Adaptive Fuzzy Dynamic Surface Control for Uncertain Nonlinear Systems

    Institute of Scientific and Technical Information of China (English)

    Xiao-Yuan Luo; Zhi-Hao Zhu; Xin-Ping Guan

    2009-01-01

    In this paper, a robust adaptive fuzzy dynamic surface control for a class of uncertain nonlinear systems is proposed. A novel adaptive fuzzy dynamic surface model is built to approximate the uncertain nonlinear functions by only one fuzzy logic system. The approximation capability of this model is proved and the model is implemented to solve the problem that too many approximators are used in the controller design of uncertain nonlinear systems. The shortage of "explosion of complexity" in backstepping design procedure is overcome by using the proposed dynamic surface control method. It is proved by constructing appropriate Lyapunov candidates that all signals of closed-loop systems are semi-globaily uniformly ultimate bounded. Also, this novel controller stabilizes the states of uncertain nonlinear systems faster than the adaptive sliding mode controller (SMC). Two simulation examples are provided to illustrate the effectiveness of the control approach proposed in this paper.

  2. Discharging dynamics in an electrolytic cell

    Science.gov (United States)

    Feicht, Sarah E.; Frankel, Alexandra E.; Khair, Aditya S.

    2016-07-01

    We analyze the dynamics of a discharging electrolytic cell comprised of a binary symmetric electrolyte between two planar, parallel blocking electrodes. When a voltage is initially applied, ions in the electrolyte migrate towards the electrodes, forming electrical double layers. After the system reaches steady state and the external current decays to zero, the applied voltage is switched off and the cell discharges, with the ions eventually returning to a uniform spatial concentration. At voltages on the order of the thermal voltage VT=kBT /q ≃25 mV, where kB is Boltzmann's constant, T is temperature, and q is the charge of a proton, experiments on surfactant-doped nonpolar fluids observe that the temporal evolution of the external current during charging and discharging is not symmetric [V. Novotny and M. A. Hopper, J. Electrochem. Soc. 126, 925 (1979), 10.1149/1.2129195; P. Kornilovitch and Y. Jeon, J. Appl. Phys. 109, 064509 (2011), 10.1063/1.3554445]. In fact, at sufficiently large voltages (several VT), the current during discharging is no longer monotonic: it displays a "reverse peak" before decaying in magnitude to zero. We analyze the dynamics of discharging by solving the Poisson-Nernst-Planck equations governing ion transport via asymptotic and numerical techniques in three regimes. First, in the "linear regime" when the applied voltage V is formally much less than VT, the charging and discharging currents are antisymmetric in time; however, the potential and charge density profiles during charging and discharging are asymmetric. The current evolution is on the R C timescale of the cell, λDL /D , where L is the width of the cell, D is the diffusivity of ions, and λD is the Debye length. Second, in the (experimentally relevant) thin-double-layer limit ɛ =λD/L ≪1 , there is a "weakly nonlinear" regime defined by VT≲V ≲VTln(1 /ɛ ) , where the bulk salt concentration is uniform; thus the R C timescale of the evolution of the current magnitude

  3. Dynamical Systems and Control Theory Inspired by Molecular Biology

    Science.gov (United States)

    2014-10-02

    in both bacterial and eukaryotic signaling pathways. A common theme in the systems biology literature is that certain systems whose output variables...AFRL-OSR-VA-TR-2014-0282 DYNAMICAL SYSTEMS AND CONTROL THEORY INSPIRED BY MOLECULAR BIOLOGY Eduardo Sontag RUTGERS THE STATE UNIVERSITY OF NEW JERSEY...Standard Form 298 (Re . 8-98) v Prescribed by ANSI Std. Z39.18 DYNAMICAL SYSTEMS AND CONTROL THEORY INSPIRED BY MOLECULAR BIOLOGY AFOSR FA9550-11-1-0247

  4. Robustified time-optimal control of uncertain structural dynamic systems

    Science.gov (United States)

    Liu, Qiang; Wie, Bong

    1991-01-01

    A new approach for computing open-loop time-optimal control inputs for uncertain linear dynamical systems is developed. In particular, the single-axis, rest-to-rest maneuvering problem of flexible spacecraft in the presence of uncertainty in model parameters is considered. Robustified time-optimal control inputs are obtained by solving a parameter optimization problem subject to robustness constraints. A simple dynamical system with a rigid-body mode and one flexible mode is used to illustrate the concept.

  5. Dynamic control of the flow of terahertz light

    DEFF Research Database (Denmark)

    Cooke, David; Jepsen, Peter Uhd

    2009-01-01

    Guided propagation of THz light has been intensely developed recently. We describe our efforts towards dynamic, optical control of the flow of light in waveguide structures, enabling reconfigurable photonic components for the terahertz frequency range.......Guided propagation of THz light has been intensely developed recently. We describe our efforts towards dynamic, optical control of the flow of light in waveguide structures, enabling reconfigurable photonic components for the terahertz frequency range....

  6. Intrinsic dynamics induce global symmetry in network controllability.

    Science.gov (United States)

    Zhao, Chen; Wang, Wen-Xu; Liu, Yang-Yu; Slotine, Jean-Jacques

    2015-02-12

    Controlling complex networked systems to desired states is a key research goal in contemporary science. Despite recent advances in studying the impact of network topology on controllability, a comprehensive understanding of the synergistic effect of network topology and individual dynamics on controllability is still lacking. Here we offer a theoretical study with particular interest in the diversity of dynamic units characterized by different types of individual dynamics. Interestingly, we find a global symmetry accounting for the invariance of controllability with respect to exchanging the densities of any two different types of dynamic units, irrespective of the network topology. The highest controllability arises at the global symmetry point, at which different types of dynamic units are of the same density. The lowest controllability occurs when all self-loops are either completely absent or present with identical weights. These findings further improve our understanding of network controllability and have implications for devising the optimal control of complex networked systems in a wide range of fields.

  7. Handbook of electrical power system dynamics modeling, stability, and control

    CERN Document Server

    Eremia, Mircea

    2013-01-01

    Complete guidance for understanding electrical power system dynamics and blackouts This handbook offers a comprehensive and up-to-date treatment of power system dynamics. Addressing the full range of topics, from the fundamentals to the latest technologies in modeling, stability, and control, Handbook of Electrical Power System Dynamics provides engineers with hands-on guidance for understanding the phenomena leading to blackouts so they can design the most appropriate solutions for a cost-effective and reliable operation. Focusing on system dynamics, the book details

  8. Analysis and control of complex dynamical systems robust bifurcation, dynamic attractors, and network complexity

    CERN Document Server

    Imura, Jun-ichi; Ueta, Tetsushi

    2015-01-01

    This book is the first to report on theoretical breakthroughs on control of complex dynamical systems developed by collaborative researchers in the two fields of dynamical systems theory and control theory. As well, its basic point of view is of three kinds of complexity: bifurcation phenomena subject to model uncertainty, complex behavior including periodic/quasi-periodic orbits as well as chaotic orbits, and network complexity emerging from dynamical interactions between subsystems. Analysis and Control of Complex Dynamical Systems offers a valuable resource for mathematicians, physicists, and biophysicists, as well as for researchers in nonlinear science and control engineering, allowing them to develop a better fundamental understanding of the analysis and control synthesis of such complex systems.

  9. Fuzzy controller for an uncertain dynamical system

    DEFF Research Database (Denmark)

    Kulczycki, P.; Wisniewski, Rafal

    2002-01-01

    The present paper deals with the time-optimal control for mechanical systems with uncertain load. A fuzzy approach is used in the design of suboptimal feedback controllers, robust with respect to the load. Statistical kernel estimators are used for the specification of crucial parameters. The met......The present paper deals with the time-optimal control for mechanical systems with uncertain load. A fuzzy approach is used in the design of suboptimal feedback controllers, robust with respect to the load. Statistical kernel estimators are used for the specification of crucial parameters....... The methodology proposed in this work may be easily adopted to other modeling uncertainties of mechanical systems, e.g. motion resistance....

  10. Control problems of discrete-time dynamical systems

    CERN Document Server

    Hasegawa, Yasumichi

    2013-01-01

    This monograph deals with control problems of discrete-time dynamical systems which include linear and nonlinear input/output relations. It will be of popular interest to researchers, engineers and graduate students who specialized in system theory. A new method which produces manipulated inputs is presented in the sense of state control and output control. This monograph provides new results and their extensions which can also be more applicable for nonlinear dynamical systems. To present the effectiveness of the method, many numerical examples of control problems are  provided as well.

  11. Rac and Rho GTPases in cancer cell motility control

    Directory of Open Access Journals (Sweden)

    Parri Matteo

    2010-09-01

    Full Text Available Abstract Rho GTPases represent a family of small GTP-binding proteins involved in cell cytoskeleton organization, migration, transcription, and proliferation. A common theme of these processes is a dynamic reorganization of actin cytoskeleton which has now emerged as a major switch control mainly carried out by Rho and Rac GTPase subfamilies, playing an acknowledged role in adaptation of cell motility to the microenvironment. Cells exhibit three distinct modes of migration when invading the 3 D environment. Collective motility leads to movement of cohorts of cells which maintain the adherens junctions and move by photolytic degradation of matrix barriers. Single cell mesenchymal-type movement is characterized by an elongated cellular shape and again requires extracellular proteolysis and integrin engagement. In addition it depends on Rac1-mediated cell polarization and lamellipodia formation. Conversely, in amoeboid movement cells have a rounded morphology, the movement is independent from proteases but requires high Rho GTPase to drive elevated levels of actomyosin contractility. These two modes of cell movement are interconvertible and several moving cells, including tumor cells, show an high degree of plasticity in motility styles shifting ad hoc between mesenchymal or amoeboid movements. This review will focus on the role of Rac and Rho small GTPases in cell motility and in the complex relationship driving the reciprocal control between Rac and Rho granting for the opportunistic motile behaviour of aggressive cancer cells. In addition we analyse the role of these GTPases in cancer progression and metastatic dissemination.

  12. A programmable metasurface with dynamic polarization, scattering and focusing control

    Science.gov (United States)

    Yang, Huanhuan; Cao, Xiangyu; Yang, Fan; Gao, Jun; Xu, Shenheng; Li, Maokun; Chen, Xibi; Zhao, Yi; Zheng, Yuejun; Li, Sijia

    2016-10-01

    Diverse electromagnetic (EM) responses of a programmable metasurface with a relatively large scale have been investigated, where multiple functionalities are obtained on the same surface. The unit cell in the metasurface is integrated with one PIN diode, and thus a binary coded phase is realized for a single polarization. Exploiting this anisotropic characteristic, reconfigurable polarization conversion is presented first. Then the dynamic scattering performance for two kinds of sources, i.e. a plane wave and a point source, is carefully elaborated. To tailor the scattering properties, genetic algorithm, normally based on binary coding, is coupled with the scattering pattern analysis to optimize the coding matrix. Besides, inverse fast Fourier transform (IFFT) technique is also introduced to expedite the optimization process of a large metasurface. Since the coding control of each unit cell allows a local and direct modulation of EM wave, various EM phenomena including anomalous reflection, diffusion, beam steering and beam forming are successfully demonstrated by both simulations and experiments. It is worthwhile to point out that a real-time switch among these functionalities is also achieved by using a field-programmable gate array (FPGA). All the results suggest that the proposed programmable metasurface has great potentials for future applications.

  13. Ureaplasma parvum infection alters filamin a dynamics in host cells

    Directory of Open Access Journals (Sweden)

    Brown Mary B

    2011-04-01

    Full Text Available Abstract Background Ureaplasmas are among the most common bacteria isolated from the human urogenital tract. Ureaplasmas can produce asymptomatic infections or disease characterized by an exaggerated inflammatory response. Most investigations have focused on elucidating the pathogenic potential of Ureaplasma species, but little attention has been paid to understanding the mechanisms by which these organisms are capable of establishing asymptomatic infection. Methods We employed differential proteome profiling of bladder tissues from rats experimentally infected with U. parvum in order to identify host cell processes perturbed by colonization with the microbe. Tissues were grouped into four categories: sham inoculated controls, animals that spontaneously cleared infection, asymptomatic urinary tract infection (UTI, and complicated UTI. One protein that was perturbed by infection (filamin A was used to further elucidate the mechanism of U. parvum-induced disruption in human benign prostate cells (BPH-1. BPH-1 cells were evaluated by confocal microscopy, immunoblotting and ELISA. Results Bladder tissue from animals actively colonized with U. parvum displayed significant alterations in actin binding proteins (profilin 1, vinculin, α actinin, and filamin A that regulate both actin polymerization and cell cytoskeletal function pertaining to focal adhesion formation and signal transduction (Fisher's exact test, P U. parvum perturbed the regulation of filamin A. Specifically, infected BPH-1 cells exhibited a significant increase in filamin A phosphorylated at serine2152 (P ≤ 0.01, which correlated with impaired proteolysis of the protein and its normal intracellular distribution. Conclusion Filamin A dynamics were perturbed in both models of infection. Phosphorylation of filamin A occurs in response to various cell signaling cascades that regulate cell motility, differentiation, apoptosis and inflammation. Thus, this phenomenon may be a useful

  14. Dynamic behaviour of human neuroepithelial cells in the developing forebrain

    Science.gov (United States)

    Subramanian, Lakshmi; Bershteyn, Marina; Paredes, Mercedes F.; Kriegstein, Arnold R.

    2017-01-01

    To understand how diverse progenitor cells contribute to human neocortex development, we examined forebrain progenitor behaviour using timelapse imaging. Here we find that cell cycle dynamics of human neuroepithelial (NE) cells differ from radial glial (RG) cells in both primary tissue and in stem cell-derived organoids. NE cells undergoing proliferative, symmetric divisions retract their basal processes, and both daughter cells regrow a new process following cytokinesis. The mitotic retraction of the basal process is recapitulated by NE cells in cerebral organoids generated from human-induced pluripotent stem cells. In contrast, RG cells undergoing vertical cleavage retain their basal fibres throughout mitosis, both in primary tissue and in older organoids. Our findings highlight developmentally regulated changes in mitotic behaviour that may relate to the role of RG cells to provide a stable scaffold for neuronal migration, and suggest that the transition in mitotic dynamics can be studied in organoid models. PMID:28139695

  15. Asymmetric cell division during T cell development controls downstream fate

    Science.gov (United States)

    Pham, Kim; Shimoni, Raz; Charnley, Mirren; Ludford-Menting, Mandy J.; Hawkins, Edwin D.; Ramsbottom, Kelly; Oliaro, Jane; Izon, David; Ting, Stephen B.; Reynolds, Joseph; Lythe, Grant; Molina-Paris, Carmen; Melichar, Heather; Robey, Ellen; Humbert, Patrick O.; Gu, Min

    2015-01-01

    During mammalian T cell development, the requirement for expansion of many individual T cell clones, rather than merely expansion of the entire T cell population, suggests a possible role for asymmetric cell division (ACD). We show that ACD of developing T cells controls cell fate through differential inheritance of cell fate determinants Numb and α-Adaptin. ACD occurs specifically during the β-selection stage of T cell development, and subsequent divisions are predominantly symmetric. ACD is controlled by interaction with stromal cells and chemokine receptor signaling and uses a conserved network of polarity regulators. The disruption of polarity by deletion of the polarity regulator, Scribble, or the altered inheritance of fate determinants impacts subsequent fate decisions to influence the numbers of DN4 cells arising after the β-selection checkpoint. These findings indicate that ACD enables the thymic microenvironment to orchestrate fate decisions related to differentiation and self-renewal. PMID:26370500

  16. Modeling, analysis and control of fuel cell hybrid power systems

    Science.gov (United States)

    Suh, Kyung Won

    Transient performance is a key characteristic of fuel cells, that is sometimes more critical than efficiency, due to the importance of accepting unpredictable electric loads. To fulfill the transient requirement in vehicle propulsion and portable fuel cell applications, a fuel cell stack is typically coupled with a battery through a DC/DC converter to form a hybrid power system. Although many power management strategies already exist, they all rely on low level controllers that realize the power split. In this dissertation we design controllers that realize various power split strategies by directly manipulating physical actuators (low level commands). We maintain the causality of the electric dynamics (voltage and current) and investigate how the electric architecture affects the hybridization level and the power management. We first establish the performance limitations associated with a stand-alone and power-autonomous fuel cell system that is not supplemented by an additional energy storage and powers all its auxiliary components by itself. Specifically, we examine the transient performance in fuel cell power delivery as it is limited by the air supplied by a compressor driven by the fuel cell itself. The performance limitations arise from the intrinsic coupling in the fluid and electrical domain between the compressor and the fuel cell stack. Feedforward and feedback control strategies are used to demonstrate these limitations analytically and with simulations. Experimental tests on a small commercial fuel cell auxiliary power unit (APU) confirm the dynamics and the identified limitations. The dynamics associated with the integration of a fuel cell system and a DC/DC converter is then investigated. Decentralized and fully centralized (using linear quadratic techniques) controllers are designed to regulate the power system voltage and to prevent fuel cell oxygen starvation. Regulating these two performance variables is a difficult task and requires a compromise

  17. Controlling chaos in dynamical systems described by maps

    Energy Technology Data Exchange (ETDEWEB)

    Crispin, Y.; Marduel, C. [Embry-Riddle Aeronautical Univ., Daytona Beach, FL (United States)

    1994-12-31

    The problem of suppressing chaotic behavior in dynamical systems is treated using a feedback control method with limited control effort. The proposed method is validated on archetypal systems described by maps, i.e. discrete-time difference equations. The method is also applicable to dynamical systems described by flows, i.e. by systems of ordinary differential equations. Results are presented for the one-dimensional logistic map and for a two-dimensional Lotka-Volterra map describing predator-prey population dynamics. It is shown that chaos can be suppressed and the system stabilized about a period-1 fixed point of the maps.

  18. Controlling the dynamic range of a Josephson parametric amplifier

    Energy Technology Data Exchange (ETDEWEB)

    Eichler, Christopher; Wallraff, Andreas [ETH Zuerich, Department of Physics, Zuerich (Switzerland)

    2014-12-01

    One of the central challenges in the development of parametric amplifiers is the control of the dynamic range relative to its gain and bandwidth, which typically limits quantum limited amplification to signals which contain only a few photons per inverse bandwidth. Here, we discuss the control of the dynamic range of Josephson parametric amplifiers by using Josephson junction arrays. We discuss gain, bandwidth, noise, and dynamic range properties of both a transmission line and a lumped element based parametric amplifier. Based on these investigations we derive useful design criteria, which may find broad application in the development of practical parametric amplifiers. (orig.)

  19. Dynamic Flow Control Strategies of Vehicle SCR Urea Dosing System

    Institute of Scientific and Technical Information of China (English)

    LIN Wei; ZHANG Youtong; ASIF Malik

    2015-01-01

    Selective Catalyst Reduction(SCR) Urea Dosing System(UDS) directly affects the system accuracy and the dynamic response performance of a vehicle. However, the UDS dynamic response is hard to keep up with the changes of the engine’s operating conditions. That will lead to low NOX conversion efficiency or NH3 slip. In order to optimize the injection accuracy and the response speed of the UDS in dynamic conditions, an advanced control strategy based on an air-assisted volumetric UDS is presented. It covers the methods of flow compensation and switching working conditions. The strategy is authenticated on an UDS and tested in different dynamic conditions. The result shows that the control strategy discussed results in higher dynamic accuracy and faster dynamic response speed of UDS. The inject deviation range is improved from being between–8%and 10%to–4%and 2%and became more stable than before, and the dynamic response time was shortened from 200 ms to 150 ms . The ETC cycle result shows that after using the new strategy the NH3 emission is reduced by 60%, and the NOX emission remains almost unchanged. The trade-off between NOX conversion efficiency and NH3 slip is mitigated. The studied flow compensation and switching working conditions can improve the dynamic performance of the UDS significantly and make the UDS dynamic response keep up with the changes of the engine’s operating conditions quickly.

  20. Torque-stiffness-controlled dynamic walking with central pattern generators.

    Science.gov (United States)

    Huang, Yan; Vanderborght, Bram; Van Ham, Ronald; Wang, Qining

    2014-12-01

    Walking behavior is modulated by controlling joint torques in most existing passivity-based bipeds. Controlled Passive Walking with adaptable stiffness exhibits controllable natural motions and energy efficient gaits. In this paper, we propose torque-stiffness-controlled dynamic bipedal walking, which extends the concept of Controlled Passive Walking by introducing structured control parameters and a bio-inspired control method with central pattern generators. The proposed walking paradigm is beneficial in clarifying the respective effects of the external actuation and the internal natural dynamics. We present a seven-link biped model to validate the presented walking. Effects of joint torque and joint stiffness on gait selection, walking performance and walking pattern transitions are studied in simulations. The work in this paper develops a new solution of motion control of bipedal robots with adaptable stiffness and provides insights of efficient and sophisticated walking gaits of humans.

  1. Dynamical modeling of the cell cycle and cell fate emergence in Caulobacter crescentus.

    Directory of Open Access Journals (Sweden)

    César Quiñones-Valles

    Full Text Available The division of Caulobacter crescentus, a model organism for studying cell cycle and differentiation in bacteria, generates two cell types: swarmer and stalked. To complete its cycle, C. crescentus must first differentiate from the swarmer to the stalked phenotype. An important regulator involved in this process is CtrA, which operates in a gene regulatory network and coordinates many of the interactions associated to the generation of cellular asymmetry. Gaining insight into how such a differentiation phenomenon arises and how network components interact to bring about cellular behavior and function demands mathematical models and simulations. In this work, we present a dynamical model based on a generalization of the Boolean abstraction of gene expression for a minimal network controlling the cell cycle and asymmetric cell division in C. crescentus. This network was constructed from data obtained from an exhaustive search in the literature. The results of the simulations based on our model show a cyclic attractor whose configurations can be made to correspond with the current knowledge of the activity of the regulators participating in the gene network during the cell cycle. Additionally, we found two point attractors that can be interpreted in terms of the network configurations directing the two cell types. The entire network is shown to be operating close to the critical regime, which means that it is robust enough to perturbations on dynamics of the network, but adaptable to environmental changes.

  2. Dynamical modeling of the cell cycle and cell fate emergence in Caulobacter crescentus.

    Science.gov (United States)

    Quiñones-Valles, César; Sánchez-Osorio, Ismael; Martínez-Antonio, Agustino

    2014-01-01

    The division of Caulobacter crescentus, a model organism for studying cell cycle and differentiation in bacteria, generates two cell types: swarmer and stalked. To complete its cycle, C. crescentus must first differentiate from the swarmer to the stalked phenotype. An important regulator involved in this process is CtrA, which operates in a gene regulatory network and coordinates many of the interactions associated to the generation of cellular asymmetry. Gaining insight into how such a differentiation phenomenon arises and how network components interact to bring about cellular behavior and function demands mathematical models and simulations. In this work, we present a dynamical model based on a generalization of the Boolean abstraction of gene expression for a minimal network controlling the cell cycle and asymmetric cell division in C. crescentus. This network was constructed from data obtained from an exhaustive search in the literature. The results of the simulations based on our model show a cyclic attractor whose configurations can be made to correspond with the current knowledge of the activity of the regulators participating in the gene network during the cell cycle. Additionally, we found two point attractors that can be interpreted in terms of the network configurations directing the two cell types. The entire network is shown to be operating close to the critical regime, which means that it is robust enough to perturbations on dynamics of the network, but adaptable to environmental changes.

  3. A Fuzzy-Neural Network Control of Nonlinear Dynamic Systems

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    In this paper,an adaptive dynamic control scheme based on a fuzzy neural network is presented,that presents utilizes both feed-forward and feedback controller elements.The former of the two elements comprises a neural network with both identification and control role,and the latter is a fuzzy neural algorithm,which is introduced to provide additional control enhancement.The feedforward controller provides only coarse control,whereas the feedback oontroller can generate on-line conditional proposition rule automatically to improve the overall control action.These properties make the design very versatile and applicable to a range of industrial applications.

  4. Dynamics and control of trajectory tubes theory and computation

    CERN Document Server

    Kurzhanski, Alexander B

    2014-01-01

    This monograph presents theoretical methods involving the Hamilton–Jacobi–Bellman formalism in conjunction with set-valued techniques of nonlinear analysis to solve significant problems in dynamics and control. The emphasis is on issues of reachability, feedback control  synthesis under complex state constraints, hard or double bounds on controls, and performance in finite time. Guaranteed state estimation, output feedback control, and hybrid dynamics are also discussed. Although the focus is on systems with linear structure, the authors indicate how to apply each approach to nonlinear and nonconvex systems. The main theoretical results lead to computational schemes based on extensions of ellipsoidal calculus that provide complete solutions to the problems. These computational schemes in turn yield software tools that can be applied effectively to high-dimensional systems. Dynamics and Control of Trajectory Tubes: Theory and Computation will interest graduate and senior undergraduate students, as well as...

  5. Complex systems and networks dynamics, controls and applications

    CERN Document Server

    Yu, Xinghuo; Chen, Guanrong; Yu, Wenwu

    2016-01-01

    This elementary book provides some state-of-the-art research results on broad disciplinary sciences on complex networks. It presents an in-depth study with detailed description of dynamics, controls and applications of complex networks. The contents of this book can be summarized as follows. First, the dynamics of complex networks, for example, the cluster dynamic analysis by using kernel spectral methods, community detection algorithms in bipartite networks, epidemiological modeling with demographics and epidemic spreading on multi-layer networks, are studied. Second, the controls of complex networks are investigated including topics like distributed finite-time cooperative control of multi-agent systems by applying homogenous-degree and Lyapunov methods, composite finite-time containment control for disturbed second-order multi-agent systems, fractional-order observer design of multi-agent systems, chaos control and anticontrol of complex systems via Parrondos game and many more. Third, the applications of ...

  6. Yaw control for active damping of structural dynamics

    Energy Technology Data Exchange (ETDEWEB)

    Ekelund, T. [Chalmers Univ. of Technology, Goeteborg (Sweden). Control Engineering Lab.

    1996-12-01

    Yaw torque control for reduction of structural dynamic loads in a two-bladed wind turbine is investigated. The models are obtained using rigid-body mechanics. Linear quadratic control theory is utilized for design and analysis. The analysis of two simple examples, where the teeter angle and the tower lateral bending motion are regarded, shows that a time-varying controller has some advantages compared with a time-invariant controller. 6 refs, 9 figs

  7. Nonlineat observer-controller design for dynamic positioning of ships

    Institute of Scientific and Technical Information of China (English)

    何黎明; 田作华; 施颂椒

    2004-01-01

    The main focus is nonlinear model-based dynamic positioning (DP) control system design. A nonlinear uniform global exponential stability (UGES) observer produces noise-free estimates of the position, the slowly varying environmental disturbances and the velocity, which are used in a proportional-derivative (PD) + feedforward control law. The stability of this observer-controller system is proved by introducing a specific nonlinear cascaded system. The simulation results have successfully demonstrated the performance of designed DP control system.

  8. Control uncertain continuous-time chaotic dynamical system

    Institute of Scientific and Technical Information of China (English)

    齐冬莲; 赵光宙

    2003-01-01

    The new chaos control method presented in this paper is useful for taking advantage of chaos. Based on sliding mode control theory, this paper provides a switching manifold controlling strategy of chaotic system, and also gives a kind of adaptive parameters estimated method to estimate the unknown systems' parameters by which chaotic dynamical system can be synchronized. Taking the Lorenz system as example, and with the help of this controlling strategy, we can synchronize chaotic systems with unknown parameters and different initial conditions.

  9. Control uncertain continuous-time chaotic dynamical system.

    Science.gov (United States)

    Qi, Dong-Lian; Zhao, Guang-Zhou

    2003-01-01

    The new chaos control method presented in this paper is useful for taking advantage of chaos. Based on sliding mode control theory, this paper provides a switching manifold controlling strategy of chaotic system, and also gives a kind of adaptive parameters estimated method to estimate the unknown systems' parameters by which chaotic dynamical system can be synchronized. Taking the Lorenz system as example, and with the help of this controlling strategy, we can synchronize chaotic systems with unknown parameters and different initial conditions.

  10. MODELLING AND FUZZY LOGIC CONTROL OF PEM FUEL CELL SYSTEM POWER GENERATION FOR RESIDENTIAL APPLICATION

    OpenAIRE

    Khaled MAMMAR; CHAKER, Abdelkader

    2010-01-01

    This paper presents a dynamic model of Fuel cell system for residential power generation. The models proposedinclude a fuel cell stack model, reformer model and DC/AC inverter model. More then an analytical details ofhow active and reactive power output of a proton-exchange-membrane (PEM) fuel cell system is controlled.Furthermore a fuzzy logic (FLC) controller is used to control active power of PEM fuel cell system. Thecontroller modifies the hydrogen flow feedback from the terminal load. Si...

  11. Sec16 alternative splicing dynamically controls COPII transport efficiency.

    Science.gov (United States)

    Wilhelmi, Ilka; Kanski, Regina; Neumann, Alexander; Herdt, Olga; Hoff, Florian; Jacob, Ralf; Preußner, Marco; Heyd, Florian

    2016-08-05

    The transport of secretory proteins from the endoplasmic reticulum (ER) to the Golgi depends on COPII-coated vesicles. While the basic principles of the COPII machinery have been identified, it remains largely unknown how COPII transport is regulated to accommodate tissue- or activation-specific differences in cargo load and identity. Here we show that activation-induced alternative splicing of Sec16 controls adaptation of COPII transport to increased secretory cargo upon T-cell activation. Using splice-site blocking morpholinos and CRISPR/Cas9-mediated genome engineering, we show that the number of ER exit sites, COPII dynamics and transport efficiency depend on Sec16 alternative splicing. As the mechanistic basis, we suggest the C-terminal Sec16 domain to be a splicing-controlled protein interaction platform, with individual isoforms showing differential abilities to recruit COPII components. Our work connects the COPII pathway with alternative splicing, adding a new regulatory layer to protein secretion and its adaptation to changing cellular environments.

  12. Performance, Career Dynamics, and Span of Control

    DEFF Research Database (Denmark)

    Smeets, Valerie Anne Rolande; Waldman, Michael; Warzynski, Frederic Michel Patrick

    . In this paper we first extend the theoretical literature on the scale-of-operations effect to allow firms’ beliefs concerning a manager’s ability to evolve over the manager’s career, where much of our focus is the determinants of span of control. We then empirically investigate testable predictions from......There is an extensive theoretical literature based on what is called the scale-of-operations effect, i.e., the idea that the return to managerial ability is higher the more resources the manager influences with his or her decisions. This idea leads to various testable predictions including...... that higher ability managers should supervise more subordinates, or equivalently, have a larger span of control. And although some of this theory’s predictions have been empirically investigated, there has been little systematic investigation of the theory’s predictions concerning span of control...

  13. Developmental control of cell division

    NARCIS (Netherlands)

    Boxem, M. (Mike)

    2002-01-01

    During development of multicellular organisms, cell divisions need to be coordinated with the developmental program of the entire organism. Although the mechanisms that drive cells through the division cycle are well understood, very little is known about the pathways that link extracellular signals

  14. Developmental control of cell division

    NARCIS (Netherlands)

    Boxem, M. (Mike)

    2002-01-01

    During development of multicellular organisms, cell divisions need to be coordinated with the developmental program of the entire organism. Although the mechanisms that drive cells through the division cycle are well understood, very little is known about the pathways that link extracellular signals

  15. Dynamics and Controls of a Conceptual Jovian Moon Tour Spacecraft

    Science.gov (United States)

    Quadrelli, Marco B.; Mettler, Edward; Langmaier, Jerry K.

    2004-01-01

    The dynamics and control challenges presented by a conceptual Jovian Moon Tour spacecraft are summarized in this paper. Attitude and orbital dynamics interactions are present due to the designed low-thrust trajectory, and controls structure interactions are also present due to the non-collocated sensor-actuator pairs on board the flexible spacecraft. A finite-element based simulation model is described which is capable of handling the complex orbital and attitude dynamics arising during the low-thrust spiraling maneuvers of the spacecraft. A few numerical simulations demonstrate that some of the challenges hitherto identified can be faced via integrated dynamics and control analysis, and that reasonable assessments of the pointing performance can be made.

  16. Modest Interference with Actin Dynamics in Primary T Cell Activation by Antigen Presenting Cells Preferentially Affects Lamellal Signaling.

    Directory of Open Access Journals (Sweden)

    Kole T Roybal

    Full Text Available Dynamic subcellular distributions of signaling system components are critical regulators of cellular signal transduction through their control of molecular interactions. Understanding how signaling activity depends on such distributions and the cellular structures driving them is required for comprehensive insight into signal transduction. In the activation of primary murine T cells by antigen presenting cells (APC signaling intermediates associate with various subcellular structures, prominently a transient, wide, and actin-associated lamellum extending from an interdigitated T cell:APC interface several micrometers into the T cell. While actin dynamics are well established as general regulators of cellular organization, their role in controlling signaling organization in primary T cell:APC couples and the specific cellular structures driving it is unresolved. Using modest interference with actin dynamics with a low concentration of Jasplakinolide as corroborated by costimulation blockade we show that T cell actin preferentially controls lamellal signaling localization and activity leading downstream to calcium signaling. Lamellal localization repeatedly related to efficient T cell function. This suggests that the transient lamellal actin matrix regulates T cell signaling associations that facilitate T cell activation.

  17. Dedicated EGR engine with dynamic load control

    Science.gov (United States)

    Hayman, Alan W.; McAlpine, Robert S.; Keating, Edward J.

    2016-09-06

    An internal combustion engine comprises a first engine bank and a second engine bank. A first intake valve is disposed in an intake port of a cylinder of the first engine bank, and is configured for metering the first flow of combustion air by periodically opening and closing according to a first intake valve lift and duration characteristic. A variable valve train control mechanism is configured for affecting the first intake valve lift and duration characteristic. Either a lift or duration of the first intake valve is modulated so as to satisfy an EGR control criterion.

  18. Attitude Dynamics and Control of Solar Sails

    Science.gov (United States)

    Sperber, Evan

    Solar sails are space vehicles that rely on solar radiation pressure in order to generate forces for thrust and attitude control torques. They exhibit characteristics such as large moments of inertia, fragility of various system components, and long mission durations that make attitude control a particularly difficult engineering problem. Thrust vector control (TVC) is a family of sailcraft attitude control techniques that is on a short list of strategies thought to be suitable for the primary attitude control of solar sails. Every sailcraft TVC device functions by manipulating the relative locations of the composite mass center (cm) of the sailcraft and the center of pressure (cp) of at least one of its reflectors. Relative displacement of these two points results in body torques that can be used to steer the sailcraft. This dissertation presents a strategy for the large-angle reorientation of a sailcraft using TVC. Two forms of TVC, namely the panel and ballast mass translation methods are well represented in the literature, while rigorous studies regarding a third form, gimballed mass rotation, are conspicuously absent. The gimballed mass method is physically realized by placing a ballast mass, commonly the sailcraft's scientific payload, at the tip of a gimballed boom that has its base fixed at some point on the sailcraft. A TVC algorithm will then strategically manipulate the payload boom's gimbal angles, thereby changing the projection of the sailcraft cm in the plane of the sail. This research demonstrates effective three-axis attitude control of a model sailcraft using numerical simulation of its nonlinear equations of motion. The particular TVC algorithm developed herein involves two phases---the first phase selects appropriate gimbal rates with the objective that the sailcraft be placed in the neighborhood of its target orientation. It was discovered, however that concomitantly minimizing attitude error as well as residual body rate was not possible using

  19. Dynamic Simulation of Carbonate Fuel Cell-Gas Turbine Hybrid Systems

    Energy Technology Data Exchange (ETDEWEB)

    Roberts, R.A. (U. of California, Irvine, CA); Brouwer, J. (U. of California, Irvine, CA); Liese, E.A.; Gemmen, R.S.

    2006-04-01

    Hybrid fuel cell/gas turbine systems provide an efficient means of producing electricity from fossil fuels with ultra low emissions. However, there are many significant challenges involved in integrating the fuel cell with the gas turbine and other components of this type of system. The fuel cell and the gas turbine must maintain efficient operation and electricity production while protecting equipment during perturbations that may occur when the system is connected to the utility grid or in stand-alone mode. This paper presents recent dynamic simulation results from two laboratories focused on developing tools to aid in the design and dynamic analyses of hybrid fuel cell systems. The simulation results present the response of a carbonate fuel cell/gas turbine, or molten carbonate fuel cell/gas turbine, (MCFC/GT) hybrid system to a load demand perturbation. Initial results suggest that creative control strategies will be needed to ensure a flexible system with wide turndown and robust dynamic operation.

  20. Solar Dynamic Power System Stability Analysis and Control

    Science.gov (United States)

    Momoh, James A.; Wang, Yanchun

    1996-01-01

    The objective of this research is to conduct dynamic analysis, control design, and control performance test of solar power system. Solar power system consists of generation system and distribution network system. A bench mark system is used in this research, which includes a generator with excitation system and governor, an ac/dc converter, six DDCU's and forty-eight loads. A detailed model is used for modeling generator. Excitation system is represented by a third order model. DDCU is represented by a seventh order system. The load is modeled by the combination of constant power and constant impedance. Eigen-analysis and eigen-sensitivity analysis are used for system dynamic analysis. The effects of excitation system, governor, ac/dc converter control, and the type of load on system stability are discussed. In order to improve system transient stability, nonlinear ac/dc converter control is introduced. The direct linearization method is used for control design. The dynamic analysis results show that these controls affect system stability in different ways. The parameter coordination of controllers are recommended based on the dynamic analysis. It is concluded from the present studies that system stability is improved by the coordination of control parameters and the nonlinear ac/dc converter control stabilize system oscillation caused by the load change and system fault efficiently.

  1. Process for control of cell division

    Science.gov (United States)

    Cone, C. D., Jr. (Inventor)

    1977-01-01

    A method of controlling mitosis of biological cells was developed, which involved inducing a change in the intracellular ionic hierarchy accompanying the cellular electrical transmembrane potential difference (Esubm) of the cells. The ionic hierarchy may be varied by imposing changes on the relative concentrations of Na(+), K(+) and Cl(-), or by directly imposing changes in the physical Esubm level across the cell surface.

  2. Cdc42-mediated tubulogenesis controls cell specification

    DEFF Research Database (Denmark)

    Kesavan, Gokul; Sand, Fredrik Wolfhagen; Greiner, Thomas Uwe

    2009-01-01

    Understanding how cells polarize and coordinate tubulogenesis during organ formation is a central question in biology. Tubulogenesis often coincides with cell-lineage specification during organ development. Hence, an elementary question is whether these two processes are independently controlled......, or whether proper cell specification depends on formation of tubes. To address these fundamental questions, we have studied the functional role of Cdc42 in pancreatic tubulogenesis. We present evidence that Cdc42 is essential for tube formation, specifically for initiating microlumen formation and later...... for maintaining apical cell polarity. Finally, we show that Cdc42 controls cell specification non-cell-autonomously by providing the correct microenvironment for proper control of cell-fate choices of multipotent progenitors. For a video summary of this article, see the PaperFlick file with the Supplemental Data...

  3. Control of Orbit and Control of Chaos in a Class of Dynamic System

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    The problem of control of orbit for the dynamic system ~ + x ( 1 -x ) ( x - a ) = 0 is discussed. Any unbounded orbit of the dynamic system can be controlled to become a bounded periodic orbit by adding a periodic stepexcitation to the system. By using a nonlinear feedback control law presented in this paper the chaos of the dynamicsystem with excitation and damping is stabilized. This method is more effectual than the linear feedback control

  4. Forced dynamic position control of PMSM with DTC utilization

    Science.gov (United States)

    Malek, Michal

    2012-11-01

    Almost one and a half century after the publication of Maxwell’s On Governors, feedback theory with PID controllers in cascade structure is still an essential part of control structures of most controlled electric drives. There are a few control strategies which are “ready” to replace it but they usually miss one of the essential fundamentals of every successful approach - simplicity hand in hand with lucidity. But there is one close relative which is simple and powerful at the same time, is not excessively abstract and without complicated mathematics. The name of this technique is Forced Dynamic Control. In this paper forced dynamic control is presented together with direct torque controlled PMSM drive as unique combination of simple algorithms for inner and outer loop of cascade structure.

  5. Nonequilibrium population dynamics of phenotype conversion of cancer cells.

    Directory of Open Access Journals (Sweden)

    Joseph Xu Zhou

    Full Text Available Tumorigenesis is a dynamic biological process that involves distinct cancer cell subpopulations proliferating at different rates and interconverting between them. In this paper we proposed a mathematical framework of population dynamics that considers both distinctive growth rates and intercellular transitions between cancer cell populations. Our mathematical framework showed that both growth and transition influence the ratio of cancer cell subpopulations but the latter is more significant. We derived the condition that different cancer cell types can maintain distinctive subpopulations and we also explain why there always exists a stable fixed ratio after cell sorting based on putative surface markers. The cell fraction ratio can be shifted by changing either the growth rates of the subpopulations (Darwinism selection or by environment-instructed transitions (Lamarckism induction. This insight can help us to understand the dynamics of the heterogeneity of cancer cells and lead us to new strategies to overcome cancer drug resistance.

  6. Optimal control methods for controlling bacterial populations with persister dynamics

    Science.gov (United States)

    Cogan, N. G.

    2016-06-01

    Bacterial tolerance to antibiotics is a well-known phenomena; however, only recent studies of bacterial biofilms have shown how multifaceted tolerance really is. By joining into a structured community and offering shared protection and gene transfer, bacterial populations can protect themselves genotypically, phenotypically and physically. In this study, we collect a line of research that focuses on phenotypic (or plastic) tolerance. The dynamics of persister formation are becoming better understood, even though there are major questions that remain. The thrust of our results indicate that even without detailed description of the biological mechanisms, theoretical studies can offer strategies that can eradicate bacterial populations with existing drugs.

  7. Understanding the dynamical control of animal movement

    Science.gov (United States)

    Edwards, Donald

    2008-03-01

    Over the last 50 years, neurophysiologists have described many neural circuits that transform sensory input into motor commands, while biomechanicians and behavioral biologists have described many patterns of animal movement that occur in response to sensory input. Attempts to link these two have been frustrated by our technical inability to record from the necessary neurons in a freely behaving animal. As a result, we don't know how these neural circuits function in the closed loop context of free behavior, where the sensory and motor context changes on a millisecond time-scale. To address this problem, we have developed a software package, AnimatLab (www.AnimatLab.com), that enables users to reconstruct an animal's body and its relevant neural circuits, to link them at the sensory and motor ends, and through simulation, to test their ability to reproduce appropriate patterns of the animal's movements in a simulated Newtonian world. A Windows-based program, AnimatLab consists of a neural editor, a body editor, a world editor, stimulus and recording facilities, neural and physics engines, and an interactive 3-D graphical display. We have used AnimatLab to study three patterns of behavior: the grasshopper jump, crayfish escape, and crayfish leg movements used in postural control, walking, reaching and grasping. In each instance, the simulation helped identify constraints on both nervous function and biomechanical performance that have provided the basis for new experiments. Colleagues elsewhere have begun to use AnimatLab to study control of paw movements in cats and postural control in humans. We have also used AnimatLab simulations to guide the development of an autonomous hexapod robot in which the neural control circuitry is downloaded to the robot from the test computer.

  8. Relevant parameters in models of cell division control

    Science.gov (United States)

    Grilli, Jacopo; Osella, Matteo; Kennard, Andrew S.; Lagomarsino, Marco Cosentino

    2017-03-01

    A recent burst of dynamic single-cell data makes it possible to characterize the stochastic dynamics of cell division control in bacteria. Different models were used to propose specific mechanisms, but the links between them are poorly explored. The lack of comparative studies makes it difficult to appreciate how well any particular mechanism is supported by the data. Here, we describe a simple and generic framework in which two common formalisms can be used interchangeably: (i) a continuous-time division process described by a hazard function and (ii) a discrete-time equation describing cell size across generations (where the unit of time is a cell cycle). In our framework, this second process is a discrete-time Langevin equation with simple physical analogues. By perturbative expansion around the mean initial size (or interdivision time), we show how this framework describes a wide range of division control mechanisms, including combinations of time and size control, as well as the constant added size mechanism recently found to capture several aspects of the cell division behavior of different bacteria. As we show by analytical estimates and numerical simulations, the available data are described precisely by the first-order approximation of this expansion, i.e., by a "linear response" regime for the correction of size fluctuations. Hence, a single dimensionless parameter defines the strength and action of the division control against cell-to-cell variability (quantified by a single "noise" parameter). However, the same strength of linear response may emerge from several mechanisms, which are distinguished only by higher-order terms in the perturbative expansion. Our analytical estimate of the sample size needed to distinguish between second-order effects shows that this value is close to but larger than the values of the current datasets. These results provide a unified framework for future studies and clarify the relevant parameters at play in the control of

  9. Dynamic consistency for Stochastic Optimal Control problems

    CERN Document Server

    Carpentier, Pierre; Cohen, Guy; De Lara, Michel; Girardeau, Pierre

    2010-01-01

    For a sequence of dynamic optimization problems, we aim at discussing a notion of consistency over time. This notion can be informally introduced as follows. At the very first time step $t_0$, the decision maker formulates an optimization problem that yields optimal decision rules for all the forthcoming time step $t_0, t_1, ..., T$; at the next time step $t_1$, he is able to formulate a new optimization problem starting at time $t_1$ that yields a new sequence of optimal decision rules. This process can be continued until final time $T$ is reached. A family of optimization problems formulated in this way is said to be time consistent if the optimal strategies obtained when solving the original problem remain optimal for all subsequent problems. The notion of time consistency, well-known in the field of Economics, has been recently introduced in the context of risk measures, notably by Artzner et al. (2007) and studied in the Stochastic Programming framework by Shapiro (2009) and for Markov Decision Processes...

  10. Descending corticospinal control of intersegmental dynamics.

    Science.gov (United States)

    Gritsenko, Valeriya; Kalaska, John F; Cisek, Paul

    2011-08-17

    To make an accurate movement, the CNS has to overcome the inherent complexities of the multijoint limb. For example, interaction torques arise when motion of individual arm segments propagates to adjacent segments causing their movement without any muscle contractions. Since these passive joint torques significantly add to the overall torques generated by active muscular contractions, they must be taken into account during planning or execution of goal-directed movements. We investigated the role of the corticospinal tract in compensating for the interaction torques during arm movements in humans. Twelve subjects reached to visual targets with their arm supported by a robotic exoskeleton. Reaching to one target was accompanied by interaction torques that assisted the movement, while reaching to the other target was accompanied by interaction torques that resisted the movement. Corticospinal excitability was assessed at different times during movement using single-pulse transcranial magnetic stimulation (TMS) over the upper-arm region of M1 (primary motor cortex). We found that TMS responses in shoulder monoarticular and elbow-shoulder biarticular muscles changed together with the interaction torques during movements in which the interaction torques were resistive. In contrast, TMS responses did not correlate with assistive interaction torques or with co-contraction. This suggests that the descending motor command includes compensation for passive limb dynamics. Furthermore, our results suggest that compensation for interaction torques involves the biarticular muscles, which span both shoulder and elbow joints and are in a biomechanically advantageous position to provide such compensation.

  11. Dynamics and control of a class of underactuated mechanical systems

    NARCIS (Netherlands)

    Reyhanoglu, Mahmut; Schaft, van der Arjan; McClamroch, N. Harris; Kolmanovsky, Ilya

    1999-01-01

    This paper presents a theoretical framework for the dynamics and control of underactuated mechanical systems, defined as systems with fewer inputs than degrees of freedom. Control system formulation of underactuated mechanical systems is addressed and a class of underactuated systems characterized b

  12. Dynamics and Control of a Class of Underactuated Mechanical Systems

    NARCIS (Netherlands)

    Reyhanoglu, Mahmut; Schaft, Arjan van der; McClamroch, N. Harris; Kolmanovsky, Ilya

    1999-01-01

    This paper presents a theoretical framework for the dynamics and control of underactuated mechanical systems, defined as systems with fewer inputs than degrees of freedom. Control system formulation of underactuated mechanical systems is addressed and a class of underactuated systems characterized b

  13. Study of optimal control problems for hybrid dynamical systems

    Institute of Scientific and Technical Information of China (English)

    Gao Rui; Wang Lei; Wang Yuzhen

    2006-01-01

    From the viewpoint of continuous systems, optimal control problem is proposed for a class of controlled Hybrid dynamical systems. Then a mathematical method- HDS minimum principle is put forward, which can solve the above problem. The HDS minimum principle is proved by means of Ekeland's variational principle.

  14. Dynamic modelling and process control of ZnS precipitation

    NARCIS (Netherlands)

    König, J.; Keesman, K.J.; Veeken, A.H.M.; Lens, P.N.L.

    2006-01-01

    This paper presents the dynamic modelling and design of a control strategy for the ZnS precipitation process. During lab¿scale experiments, the sulfide concentration in a precipitator was controlled at a prespecified pS value by manipulating the flow from a buffer vessel. Batch tests showed that the

  15. Dynamic regulation of Schwann cell enhancers after peripheral nerve injury.

    Science.gov (United States)

    Hung, Holly A; Sun, Guannan; Keles, Sunduz; Svaren, John

    2015-03-13

    Myelination of the peripheral nervous system is required for axonal function and long term stability. After peripheral nerve injury, Schwann cells transition from axon myelination to a demyelinated state that supports neuronal survival and ultimately remyelination of axons. Reprogramming of gene expression patterns during development and injury responses is shaped by the actions of distal regulatory elements that integrate the actions of multiple transcription factors. We used ChIP-seq to measure changes in histone H3K27 acetylation, a mark of active enhancers, to identify enhancers in myelinating rat peripheral nerve and their dynamics after demyelinating nerve injury. Analysis of injury-induced enhancers identified enriched motifs for c-Jun, a transcription factor required for Schwann cells to support nerve regeneration. We identify a c-Jun-bound enhancer in the gene for Runx2, a transcription factor induced after nerve injury, and we show that Runx2 is required for activation of other induced genes. In contrast, enhancers that lose H3K27ac after nerve injury are enriched for binding sites of the Sox10 and early growth response 2 (Egr2/Krox20) transcription factors, which are critical determinants of Schwann cell differentiation. Egr2 expression is lost after nerve injury, and many Egr2-binding sites lose H3K27ac after nerve injury. However, the majority of Egr2-bound enhancers retain H3K27ac, indicating that other transcription factors maintain active enhancer status after nerve injury. The global epigenomic changes in H3K27ac deposition pinpoint dynamic changes in enhancers that mediate the effects of transcription factors that control Schwann cell myelination and peripheral nervous system responses to nerve injury.

  16. On the control of opinion dynamics in social networks

    Science.gov (United States)

    Liu, Zhihong; Ma, Jianfeng; Zeng, Yong; Yang, Li; Huang, Qiping; Wu, Hongliang

    2014-09-01

    This paper presents a framework to analyze the controllability of opinion dynamics in social networks using DeGroot model (DeGroot, 1974). We show how the opinion, or attitude about some common questions of interest in a population can be controlled by a committed node who consistently proselytizes the opposing opinion and is immune to influence. Some criteria are established to guarantee that opinion dynamics of networks can be perfectly or partially controlled. We also find that the opinion fluctuation is determined by the smallest negative eigenvalue of an influence matrix.

  17. On the minimax feedback control of uncertain dynamic systems.

    Science.gov (United States)

    Bertsekas, D. P.; Rhodes, I. B.

    1971-01-01

    In this paper the problem of optimal feedback control of uncertain discrete-time dynamic systems is considered where the uncertain quantities do not have a stochastic description but instead are known to belong to given sets. The problem is converted to a sequential minimax problem and dynamic programming is suggested as a general method for its solution. The notion of a sufficiently informative function, which parallels the notion of a sufficient statistic of stochastic optimal control, is introduced, and conditions under which the optimal controller decomposes into an estimator and an actuator are identified.

  18. Pulse Control Assisted Dynamical Decoupling in a Central Spin Model

    Science.gov (United States)

    Li, Zhao-Yan; Wei, Yong-Bo; Wang, Zhao-Ming; Gu, Yong-Jian; Li, Wen-Dong; Ma, Xiao-Ping

    2017-02-01

    We study pulse control assisted dynamical decoupling through a central spin model in a total Hilbert space. We find that the effective decoupling can be realized by applying a sequence of external pulses. Compared with the bang-bang control which needs infinite strength and infinitesimal short pulses, we show that there is a large parameter space that allows an effective nonperturbative dynamical control. Furthermore, our numerical calculation shows that the reliability can be held for random pulses, such as random pulse time interval or random strength.

  19. Wind turbine control systems: Dynamic model development using system identification and the fast structural dynamics code

    Energy Technology Data Exchange (ETDEWEB)

    Stuart, J.G.; Wright, A.D.; Butterfield, C.P.

    1996-10-01

    Mitigating the effects of damaging wind turbine loads and responses extends the lifetime of the turbine and, consequently, reduces the associated Cost of Energy (COE). Active control of aerodynamic devices is one option for achieving wind turbine load mitigation. Generally speaking, control system design and analysis requires a reasonable dynamic model of {open_quotes}plant,{close_quotes} (i.e., the system being controlled). This paper extends the wind turbine aileron control research, previously conducted at the National Wind Technology Center (NWTC), by presenting a more detailed development of the wind turbine dynamic model. In prior research, active aileron control designs were implemented in an existing wind turbine structural dynamics code, FAST (Fatigue, Aerodynamics, Structures, and Turbulence). In this paper, the FAST code is used, in conjunction with system identification, to generate a wind turbine dynamic model for use in active aileron control system design. The FAST code is described and an overview of the system identification technique is presented. An aileron control case study is used to demonstrate this modeling technique. The results of the case study are then used to propose ideas for generalizing this technique for creating dynamic models for other wind turbine control applications.

  20. Dual adaptive dynamic control of mobile robots using neural networks.

    Science.gov (United States)

    Bugeja, Marvin K; Fabri, Simon G; Camilleri, Liberato

    2009-02-01

    This paper proposes two novel dual adaptive neural control schemes for the dynamic control of nonholonomic mobile robots. The two schemes are developed in discrete time, and the robot's nonlinear dynamic functions are assumed to be unknown. Gaussian radial basis function and sigmoidal multilayer perceptron neural networks are used for function approximation. In each scheme, the unknown network parameters are estimated stochastically in real time, and no preliminary offline neural network training is used. In contrast to other adaptive techniques hitherto proposed in the literature on mobile robots, the dual control laws presented in this paper do not rely on the heuristic certainty equivalence property but account for the uncertainty in the estimates. This results in a major improvement in tracking performance, despite the plant uncertainty and unmodeled dynamics. Monte Carlo simulation and statistical hypothesis testing are used to illustrate the effectiveness of the two proposed stochastic controllers as applied to the trajectory-tracking problem of a differentially driven wheeled mobile robot.

  1. Positive explorers: modeling dynamic control in normal aging.

    Science.gov (United States)

    Glass, Brian D; Osman, Magda

    2017-01-01

    Situations in which there are multiple changes occurring all at once and which demand complex decisions to be made are common throughout life, but little is known about how normal aging influences performance on these types of scenarios. To determine performance differences associated with normal aging, we test older and younger adults in a dynamic control task. The task involves the control of a single output variable over time via multiple and uncertain input controls. The Single Limited Input, Dynamic Exploratory Responses (SLIDER) computational model, is implemented to determine the behavioral characteristics associated with normal aging in a dynamic control task. Model-based analysis demonstrates a unique performance signature profile associated with normal aging. Specifically, older adults exhibit a positivity effect in which they are more influenced by positively valenced feedback, congruent with previous research, as well as enhanced exploratory behavior.

  2. Position Control of Linear Synchronous Motor Drives with Exploitation of Forced Dynamics Control Principles

    Directory of Open Access Journals (Sweden)

    Jan Vittek

    2004-01-01

    Full Text Available Closed-loop position control of mechanisms directly driven by linear synchronous motors with permanent magnets is presented. The control strategy is based on forced dynamic control, which is a form of feedback linearisation, yielding a non-liner multivariable control law to obtain a prescribed linear speed dynamics together with the vector control condition of mutal orthogonality between the stator current and magnetic flux vectors (assuming perfect estimates of the plant parameters. Outer position control loop is closed via simple feedback with proportional gain. Simulations of the design control sysstem, including the drive with power electronic switching, predict the intended drive performance.

  3. Fabrication of dynamic self-assembled monolayers for cell migration and adhesion studies.

    Science.gov (United States)

    Westcott, Nathan P; Yousaf, Muhammad N

    2011-01-01

    How cells interact with the extracellular matrix (ECM) is important for a number of fundamental -processes in cell biology. However, the ECM is highly complex and in order to simplify the matrix for cell biological studies, it has been modeled with self-assembled monolayers (SAMs) of alkanethiolates on gold substrates. In this chapter, we outline procedures to create dynamic surfaces by functionalizing SAMs. SAMs based on quinone, oxyamine, and alcohol-terminated thiols were used to immobilize cell adhesive peptides with spatial control. Cells were seeded to these surfaces to provide cell co-culture -patterns suitable for biological studies.

  4. Rotation of single live mammalian cells using dynamic holographic optical tweezers

    Science.gov (United States)

    Bin Cao; Kelbauskas, Laimonas; Chan, Samantha; Shetty, Rishabh M.; Smith, Dean; Meldrum, Deirdre R.

    2017-05-01

    We report on a method for rotating single mammalian cells about an axis perpendicular to the optical system axis through the imaging plane using dynamic holographic optical tweezers (HOTs). Two optical traps are created on the opposite edges of a mammalian cell and are continuously transitioned through the imaging plane along the circumference of the cell in opposite directions, thus providing the torque to rotate the cell in a controlled fashion. The method enables a complete 360° rotation of live single mammalian cells with spherical or near-to spherical shape in 3D space, and represents a useful tool suitable for the single cell analysis field, including tomographic imaging.

  5. Maximal entanglement achievable by controlled dynamics

    CERN Document Server

    Serafini, Alessio

    2009-01-01

    We consider the feedback control of quantum systems comprised of any number of bosonic degrees of freedom. We derive a general upper bound for the logarithmic negativity achievable, at steady state, with continuous Gaussian measurements on the environment and linear driving on the system. Our results apply to rotating wave system-bath couplings and to any quadratic system's Hamiltonian. Furthermore, we apply this upper bound to parametric processes, show it to be tight, and compare it to feedback strategies limited to local measurements.

  6. Desmosome dynamics in migrating epithelial cells requires the actin cytoskeleton

    Science.gov (United States)

    Roberts, Brett J.; Pashaj, Anjeza; Johnson, Keith R.; Wahl, James K.

    2011-01-01

    Re-modeling of epithelial tissues requires that the cells in the tissue rearrange their adhesive contacts in order to allow cells to migrate relative to neighboring cells. Desmosomes are prominent adhesive structures found in a variety of epithelial tissues that are believed to inhibit cell migration and invasion. Mechanisms regulating desmosome assembly and stability in migrating cells are largely unknown. In this study we established a cell culture model to examine the fate of desmosomal components during scratch wound migration. Desmosomes are rapidly assembled between epithelial cells at the lateral edges of migrating cells and structures are transported in a retrograde fashion while the structures become larger and mature. Desmosome assembly and dynamics in this system are dependent on the actin cytoskeleton prior to being associated with the keratin intermediate filament cytoskeleton. These studies extend our understanding of desmosome assembly and provide a system to examine desmosome assembly and dynamics during epithelial cell migration. PMID:21945137

  7. On the quasi-controllability of continuous-time dynamic fuzzy control systems

    Energy Technology Data Exchange (ETDEWEB)

    Feng Yuhu [Department of Applied Mathematics, Dong Hua University, Shanghai 200051 (China)]. E-mail: yhfeng@dhu.edu.cn; Hu Liangjian [Department of Applied Mathematics, Dong Hua University, Shanghai 200051 (China)

    2006-10-15

    This paper gives the controllability analysis of continuous-time dynamic fuzzy control system from the aspect of fuzzy differential equations. The fuzzy state is different from the crisp state, as the counterpart of the controllability concept in the classical control theory, the controllable target state must be restricted within some limits. Hence, the concepts of admissible controllable state subset and quasi-controllability are introduced to describe the controllability property for fuzzy control system. The sufficient and necessary conditions for the fuzzy control system to be quasi-controllable are obtained and some examples are given to demonstrate the problems discussed in this paper.

  8. Novel coupling scheme to control dynamics of coupled discrete systems

    Science.gov (United States)

    Shekatkar, Snehal M.; Ambika, G.

    2015-08-01

    We present a new coupling scheme to control spatio-temporal patterns and chimeras on 1-d and 2-d lattices and random networks of discrete dynamical systems. The scheme involves coupling with an external lattice or network of damped systems. When the system network and external network are set in a feedback loop, the system network can be controlled to a homogeneous steady state or synchronized periodic state with suppression of the chaotic dynamics of the individual units. The control scheme has the advantage that its design does not require any prior information about the system dynamics or its parameters and works effectively for a range of parameters of the control network. We analyze the stability of the controlled steady state or amplitude death state of lattices using the theory of circulant matrices and Routh-Hurwitz criterion for discrete systems and this helps to isolate regions of effective control in the relevant parameter planes. The conditions thus obtained are found to agree well with those obtained from direct numerical simulations in the specific context of lattices with logistic map and Henon map as on-site system dynamics. We show how chimera states developed in an experimentally realizable 2-d lattice can be controlled using this scheme. We propose this mechanism can provide a phenomenological model for the control of spatio-temporal patterns in coupled neurons due to non-synaptic coupling with the extra cellular medium. We extend the control scheme to regulate dynamics on random networks and adapt the master stability function method to analyze the stability of the controlled state for various topologies and coupling strengths.

  9. Multispectral fingerprinting for improved in vivo cell dynamics analysis

    Directory of Open Access Journals (Sweden)

    Cooper Cameron HJ

    2010-09-01

    Full Text Available Abstract Background Tracing cell dynamics in the embryo becomes tremendously difficult when cell trajectories cross in space and time and tissue density obscure individual cell borders. Here, we used the chick neural crest (NC as a model to test multicolor cell labeling and multispectral confocal imaging strategies to overcome these roadblocks. Results We found that multicolor nuclear cell labeling and multispectral imaging led to improved resolution of in vivo NC cell identification by providing a unique spectral identity for each cell. NC cell spectral identity allowed for more accurate cell tracking and was consistent during short term time-lapse imaging sessions. Computer model simulations predicted significantly better object counting for increasing cell densities in 3-color compared to 1-color nuclear cell labeling. To better resolve cell contacts, we show that a combination of 2-color membrane and 1-color nuclear cell labeling dramatically improved the semi-automated analysis of NC cell interactions, yet preserved the ability to track cell movements. We also found channel versus lambda scanning of multicolor labeled embryos significantly reduced the time and effort of image acquisition and analysis of large 3D volume data sets. Conclusions Our results reveal that multicolor cell labeling and multispectral imaging provide a cellular fingerprint that may uniquely determine a cell's position within the embryo. Together, these methods offer a spectral toolbox to resolve in vivo cell dynamics in unprecedented detail.

  10. Adaptive Dynamic Programming for Control Algorithms and Stability

    CERN Document Server

    Zhang, Huaguang; Luo, Yanhong; Wang, Ding

    2013-01-01

    There are many methods of stable controller design for nonlinear systems. In seeking to go beyond the minimum requirement of stability, Adaptive Dynamic Programming for Control approaches the challenging topic of optimal control for nonlinear systems using the tools of  adaptive dynamic programming (ADP). The range of systems treated is extensive; affine, switched, singularly perturbed and time-delay nonlinear systems are discussed as are the uses of neural networks and techniques of value and policy iteration. The text features three main aspects of ADP in which the methods proposed for stabilization and for tracking and games benefit from the incorporation of optimal control methods: • infinite-horizon control for which the difficulty of solving partial differential Hamilton–Jacobi–Bellman equations directly is overcome, and  proof provided that the iterative value function updating sequence converges to the infimum of all the value functions obtained by admissible control law sequences; • finite-...

  11. Controllability of open quantum systems with Kraus-map dynamics

    Energy Technology Data Exchange (ETDEWEB)

    Wu Rong; Pechen, Alexander; Brif, Constantin; Rabitz, Herschel [Department of Chemistry, Princeton University, Princeton, NJ 08544 (United States)

    2007-05-25

    This paper presents a constructive proof of complete kinematic state controllability of finite-dimensional open quantum systems whose dynamics are represented by Kraus maps. For any pair of states (pure or mixed) on the Hilbert space of the system, we explicitly show how to construct a Kraus map that transforms one state into another. Moreover, we prove by construction the existence of a Kraus map that transforms all initial states into a predefined target state (such a process may be used, for example, in quantum information dilution). Thus, in sharp contrast to unitary control, Kraus-map dynamics allows for the design of controls which are robust to variations in the initial state of the system. The capabilities of non-unitary control for population transfer between pure states illustrated for an example of a two-level system by constructing a family of non-unitary Kraus maps to transform one pure state into another. The problem of dynamic state controllability of open quantum systems (i.e., controllability of state-to-state transformations, given a set of available dynamical resources such as coherent controls, incoherent interactions with the environment, and measurements) is also discussed.

  12. Controlled shear filtration: A novel technique for animal cell separation.

    Science.gov (United States)

    Vogel, J H; Kroner, K H

    1999-06-20

    A novel rotary microfiltration technique specifically suited for the separation of animal cells has been developed. The concept allows the independent adjustment of wall shear stress, transmembrane pressure, and residence time, allowing straightforward optimization of the microfiltration process. By using a smooth, conically shaped rotor, it is possible to establish a controlled shear field in which animal cells experience a significant hydrodynamic lift away from the membrane surface. It is shown in preliminary experiments that shear-induced cell-rupture speeds up membrane clogging and that cell debris poses the most significant problem in harvesting of BHK cell cultures by dynamic microfiltration. However, a threshold value of shear stability exists which depends on the frequency of passing the shear field, the residence time in the shear field, as well as on cell status. By operating close to this threshold value, cell viability can be maintained while concentration polarization is efficiently minimized. By applying this concept, it is possible to attain flux rates several times higher compared to conventional crossflow filtration. Controlled shear filtration (CSF) can be used for batch harvesting as well as for cell retention in high cell density systems. In batch harvesting of hIL-2 from rBHK cell culture, a constant flux rate of 290 L h-1 m-2 has been adjusted without indication of membrane clogging or fouling.

  13. Feasibility of Applying Controllable Lubrication to Dynamically Loaded Journal Bearings

    DEFF Research Database (Denmark)

    Estupinan, Edgar Alberto; Santos, Ilmar

    2009-01-01

    A multibody dynamic model of the main mechanical components of a hermetic reciprocating compressor is presented in this work. Considering that some of the mechanical elements are interconnected via thin fluid films, the multibody dynamic model is coupled to the equations from the dynamics...... levels, wear and power losses of the system components. From the point of view of actively controlled lubrication and specifically for the case of dynamically loaded journal bearings, the injection pressure should be controlled in time domain. However, taking into account that the gas pressure...... and reaction forces in a reciprocating compressor have a cyclic behavior, periodic oil pressure injection rules based on the instantaneous crank angle and load bearing condition can be established. In this paper, several bearing configurations working under different oil pressure injection rules conditions...

  14. Dynamic Simulation of a Proton Exchange Membrane Fuel Cell System For Automotive Applications

    DEFF Research Database (Denmark)

    Rabbani, Raja Abid; Rokni, Masoud

    2012-01-01

    A dynamic model of the PEMFC system is developed to investigate the behaviour and transient response of the fuel cell system for automotive applications. The system accounts for the fuel cell stack with coolant, humidifier, heat exchangers and pumps. Governing equations for fuel cell and humidifier...... sufficient insight for further in-depth analysis of PEMFC and prove to be a basis for efficient control and design methodologies....

  15. Dynamics and control of Lorentz-augmented spacecraft relative motion

    CERN Document Server

    Yan, Ye; Yang, Yueneng

    2017-01-01

    This book develops a dynamical model of the orbital motion of Lorentz spacecraft in both unperturbed and J2-perturbed environments. It explicitly discusses three kinds of typical space missions involving relative orbital control: spacecraft hovering, rendezvous, and formation flying. Subsequently, it puts forward designs for both open-loop and closed-loop control schemes propelled or augmented by the geomagnetic Lorentz force. These control schemes are entirely novel and represent a significantly departure from previous approaches.

  16. System Dynamics and Adaptive Control for MEMS Gyroscope Sensor

    OpenAIRE

    Juntao Fei; Hongfei Ding

    2010-01-01

    This paper presents an adaptive control approach for Micro-Electro-Mechanical Systems (MEMS) z-axis gyroscope sensor. The dynamical model of MEMS gyroscope sensor is derived and adaptive state tracking control for MEMS gyroscope is developed. The proposed adaptive control approaches can estimate the angular velocity and the damping and stiffness coefficients including the coupling terms due to the fabrication imperfection. The stability of the closed-loop systems is established with the propo...

  17. System Dynamics and Adaptive Control for MEMS Gyroscope Sensor

    OpenAIRE

    Juntao Fei; Hongfei Ding

    2011-01-01

    This paper presents an adaptive control approach for Micro-Electro-Mechanical Systems (MEMS) z-axis gyroscope sensor. The dynamical model of MEMS gyroscope sensor is derived and adaptive state tracking control for MEMS gyroscope is developed. The proposed adaptive control approaches can estimate the angular velocity and the damping and stiffness coefficients including the coupling terms due to the fabrication imperfection. The stability of the closed-loop systems is established with the propo...

  18. On Chattering-Free Dynamic Sliding Mode Controller Design

    OpenAIRE

    Jeang-Lin Chang

    2012-01-01

    For a class of linear MIMO uncertain systems, a dynamic sliding mode control algorithm that avoids the chattering problem is proposed in this paper. Without using any differentiator, we develop a modified asymptotically stable second-order sliding mode control law in which the proposed controller can guarantee the finite time convergence to the sliding mode and can show that the system states asymptotically approach to zero. Finally, a numerical example is explained for demonstrating the appl...

  19. Controllable subspace of edge dynamics in complex networks

    Science.gov (United States)

    Pang, Shao-Peng; Hao, Fei

    2017-09-01

    For the edge dynamics in some real networks, it may be neither feasible nor necessary to be fully controlled. An accompanying issue is that, when the external signal is applied to a few nodes or even a single node, how many edges can be controlled? In this paper, for the edge dynamics system, we propose a theoretical framework to determine the controllable subspace and calculate its generic dimension based on the integer linear programming. This framework allows us not only to analyze the control centrality, i.e., the ability of a node to control, but also to uncover the controllable centrality, i.e., the propensity of an edge to be controllable. The simulation results and analytic calculation show that dense and homogeneous networks tend to have larger control centrality of nodes and controllable centrality of edges, but the negatively correlated in- and out-degrees of nodes or edges can reduce the two centrality. The positive correlation between the control centrality of node and its out-degree leads to that the distribution of control centrality, instead of that of controllable centrality, is encoded by the out-degree distribution of networks. Meanwhile, the positive correlation indicates that the nodes with high out-degree tend to play more important roles in control.

  20. Adaptive control of ROVs with actuator dynamics and saturation

    Directory of Open Access Journals (Sweden)

    Ola-Erik Fjellstad

    1992-07-01

    Full Text Available A direct model reference adaptive controller (MRAC is derived for an underwater vehicle with significant thruster dynamics and limited thruster power. The reference model decomposition (RMD technique is used to compensate for the thruster dynamics. A reference model adjustment (RMA technique modifying the reference model acceleration is used to avoid thruster saturation. The design methods are simulated for the yawing motion of an underwater vehicle.

  1. Multiple model-informed open-loop control of uncertain intracellular signaling dynamics.

    Directory of Open Access Journals (Sweden)

    Jeffrey P Perley

    2014-04-01

    Full Text Available Computational approaches to tune the activation of intracellular signal transduction pathways both predictably and selectively will enable researchers to explore and interrogate cell biology with unprecedented precision. Techniques to control complex nonlinear systems typically involve the application of control theory to a descriptive mathematical model. For cellular processes, however, measurement assays tend to be too time consuming for real-time feedback control and models offer rough approximations of the biological reality, thus limiting their utility when considered in isolation. We overcome these problems by combining nonlinear model predictive control with a novel adaptive weighting algorithm that blends predictions from multiple models to derive a compromise open-loop control sequence. The proposed strategy uses weight maps to inform the controller of the tendency for models to differ in their ability to accurately reproduce the system dynamics under different experimental perturbations (i.e. control inputs. These maps, which characterize the changing model likelihoods over the admissible control input space, are constructed using preexisting experimental data and used to produce a model-based open-loop control framework. In effect, the proposed method designs a sequence of control inputs that force the signaling dynamics along a predefined temporal response without measurement feedback while mitigating the effects of model uncertainty. We demonstrate this technique on the well-known Erk/MAPK signaling pathway in T cells. In silico assessment demonstrates that this approach successfully reduces target tracking error by 52% or better when compared with single model-based controllers and non-adaptive multiple model-based controllers. In vitro implementation of the proposed approach in Jurkat cells confirms a 63% reduction in tracking error when compared with the best of the single-model controllers. This study provides an experimentally

  2. Multiple model-informed open-loop control of uncertain intracellular signaling dynamics.

    Science.gov (United States)

    Perley, Jeffrey P; Mikolajczak, Judith; Harrison, Marietta L; Buzzard, Gregery T; Rundell, Ann E

    2014-04-01

    Computational approaches to tune the activation of intracellular signal transduction pathways both predictably and selectively will enable researchers to explore and interrogate cell biology with unprecedented precision. Techniques to control complex nonlinear systems typically involve the application of control theory to a descriptive mathematical model. For cellular processes, however, measurement assays tend to be too time consuming for real-time feedback control and models offer rough approximations of the biological reality, thus limiting their utility when considered in isolation. We overcome these problems by combining nonlinear model predictive control with a novel adaptive weighting algorithm that blends predictions from multiple models to derive a compromise open-loop control sequence. The proposed strategy uses weight maps to inform the controller of the tendency for models to differ in their ability to accurately reproduce the system dynamics under different experimental perturbations (i.e. control inputs). These maps, which characterize the changing model likelihoods over the admissible control input space, are constructed using preexisting experimental data and used to produce a model-based open-loop control framework. In effect, the proposed method designs a sequence of control inputs that force the signaling dynamics along a predefined temporal response without measurement feedback while mitigating the effects of model uncertainty. We demonstrate this technique on the well-known Erk/MAPK signaling pathway in T cells. In silico assessment demonstrates that this approach successfully reduces target tracking error by 52% or better when compared with single model-based controllers and non-adaptive multiple model-based controllers. In vitro implementation of the proposed approach in Jurkat cells confirms a 63% reduction in tracking error when compared with the best of the single-model controllers. This study provides an experimentally

  3. Development and dynamics of cell polarity at a glance.

    Science.gov (United States)

    Campanale, Joseph P; Sun, Thomas Y; Montell, Denise J

    2017-04-01

    Cells exhibit morphological and molecular asymmetries that are broadly categorized as cell polarity. The cell polarity established in early embryos prefigures the macroscopic anatomical asymmetries characteristic of adult animals. For example, eggs and early embryos have polarized distributions of RNAs and proteins that generate global anterior/posterior and dorsal/ventral axes. The molecular programs that polarize embryos are subsequently reused in multiple contexts. Epithelial cells require apical/basal polarity to establish their barrier function. Migrating cells polarize in the direction of movement, creating distinct leading and trailing structures. Asymmetrically dividing stem cells partition different molecules between themselves and their daughter cells. Cell polarity can develop de novo, be maintained through rounds of cell division and be dynamically remodeled. In this Cell Science at a Glance review and poster, we describe molecular asymmetries that underlie cell polarity in several cellular contexts. We highlight multiple developmental systems that first establish cell/developmental polarity, and then maintain it. Our poster showcases repeated use of the Par, Scribble and Crumbs polarity complexes, which drive the development of cell polarity in many cell types and organisms. We then briefly discuss the diverse and dynamic changes in cell polarity that occur during cell migration, asymmetric cell division and in planar polarized tissues.

  4. Impulsive and hybrid dynamical systems stability, dissipativity, and control

    CERN Document Server

    Haddad, Wassim M; Nersesov, Sergey G

    2014-01-01

    This book develops a general analysis and synthesis framework for impulsive and hybrid dynamical systems. Such a framework is imperative for modern complex engineering systems that involve interacting continuous-time and discrete-time dynamics with multiple modes of operation that place stringent demands on controller design and require implementation of increasing complexity--whether advanced high-performance tactical fighter aircraft and space vehicles, variable-cycle gas turbine engines, or air and ground transportation systems. Impulsive and Hybrid Dynamical Systems goes beyond similar

  5. System Dynamics and Adaptive Control for MEMS Gyroscope Sensor

    Directory of Open Access Journals (Sweden)

    Juntao Fei

    2011-01-01

    Full Text Available This paper presents an adaptive control approach for Micro-Electro-Mechanical Systems (MEMS z-axis gyroscope sensor. The dynamical model of MEMS gyroscope sensor is derived and adaptive state tracking control for MEMS gyroscope is developed. The proposed adaptive control approaches can estimate the angular velocity and the damping and stiffness coefficients including the coupling terms due to the fabrication imperfection. The stability of the closed-loop systems is established with the proposed adaptive control strategy. Numerical simulation is investigated to verify the effectiveness of the proposed control scheme.

  6. The dynamics and control of large flexible space structures

    Science.gov (United States)

    Bainum, P. M.; Krishna, R.; Kumar, V. K.; Reddy, A. S. S. R.

    1981-01-01

    The dynamics and attitude and shape control of very large, inherently flexible spacecraft systems were investigated. Increasingly more complex examples were examined, beginning with a uniform free-free beam, next a free-free uniform plate/platform and finally by considering a thin shallow spherical shell structure in orbit. The effects devices were modeled. For given sets of assumed actuator locations, the controllability of these systems was first established. Control laws for each of the actuators were developed based on decoupling techniques (including distributed modal control) pole placement algorithms and a application of the linear regulator problem for optical control theory.

  7. Controllability analysis of decentralised linear controllers for polymeric fuel cells

    Energy Technology Data Exchange (ETDEWEB)

    Serra, Maria; Aguado, Joaquin; Ansede, Xavier; Riera, Jordi [Institut de Robotica i Informatica Industrial, Universitat Politecnica de Catalunya - Consejo Superior de Investigaciones Cientificas, C. Llorens i Artigas 4, 08028 Barcelona (Spain)

    2005-10-10

    This work deals with the control of polymeric fuel cells. It includes a linear analysis of the system at different operating points, the comparison and selection of different control structures, and the validation of the controlled system by simulation. The work is based on a complex non linear model which has been linearised at several operating points. The linear analysis tools used are the Morari resiliency index, the condition number, and the relative gain array. These techniques are employed to compare the controllability of the system with different control structures and at different operating conditions. According to the results, the most promising control structures are selected and their performance with PI based diagonal controllers is evaluated through simulations with the complete non linear model. The range of operability of the examined control structures is compared. Conclusions indicate good performance of several diagonal linear controllers. However, very few have a wide operability range. (author)

  8. Exploring Neural Cell Dynamics with Digital Holographic Microscopy

    KAUST Repository

    Marquet, Pierre

    2013-04-21

    In this talk, I will present how digital holographic microscopy, as a powerful quantitative phase technique, can non-invasively measure cell dynamics and especially resolve local neuronal network activity through simultaneous multiple site optical recording.

  9. Hybrid internal model control and proportional control of chaotic dynamical systems

    Institute of Scientific and Technical Information of China (English)

    齐冬莲; 姚良宾

    2004-01-01

    A new chaos control method is proposed to take advantage of chaos or avoid it. The hybrid Internal Model Control and Proportional Control learning scheme are introduced. In order to gain the desired robust performance and ensure the system's stability, Adaptive Momentum Algorithms are also developed. Through properly designing the neural network plant model and neural network controller, the chaotic dynamical systems are controlled while the parameters of the BP neural network are modified. Taking the Lorenz chaotic system as example, the results show that chaotic dynamical systems can be stabilized at the desired orbits by this control strategy.

  10. Hybrid internal model control and proportional control of chaotic dynamical systems.

    Science.gov (United States)

    Qi, Dong-lian; Yao, Liang-bin

    2004-01-01

    A new chaos control method is proposed to take advantage of chaos or avoid it. The hybrid Internal Model Control and Proportional Control learning scheme are introduced. In order to gain the desired robust performance and ensure the system's stability, Adaptive Momentum Algorithms are also developed. Through properly designing the neural network plant model and neural network controller, the chaotic dynamical systems are controlled while the parameters of the BP neural network are modified. Taking the Lorenz chaotic system as example, the results show that chaotic dynamical systems can be stabilized at the desired orbits by this control strategy.

  11. Modeling and Simulating Dynamics of Missiles with Deflectable Nose Control

    Institute of Scientific and Technical Information of China (English)

    Gao Yuan; Gu Liangxian; Pan Lei

    2009-01-01

    This article investigates the dynamic characteristics of deflectable nose missiles with rotary single-channel control. After introduction of effective attack and sideslip angles as well as quasi-body coordinates based on the spin characteristics of the missile's body, an integrated rigid kinetic model of missile with deflectable nose control is set up in the quasi-body coordinates considering the interaction between the missile's nose and body by using rootless multi-rigid-body system dynamics and is linearized. Then an analysis with simulation is conducted to investigate the coupling characteristics between the channels, the influences of nose deflection on the body and the dynamic characteristics of missile's body. The results indicate that various channels of missiles with deflectable nose control are coupled cross-linked; the nose deflection tends to make the body move in the opposite direction and, finally, evidences the correctness and reasonability of the kinetic model proposed by this article.

  12. Controllability of Weighted and Directed Networks with Nonidentical Node Dynamics

    Directory of Open Access Journals (Sweden)

    Linying Xiang

    2013-01-01

    Full Text Available The concept of controllability from control theory is applied to weighted and directed networks with heterogenous linear or linearized node dynamics subject to exogenous inputs, where the nodes are grouped into leaders and followers. Under this framework, the controllability of the controlled network can be decomposed into two independent problems: the controllability of the isolated leader subsystem and the controllability of the extended follower subsystem. Some necessary and/or sufficient conditions for the controllability of the leader-follower network are derived based on matrix theory and graph theory. In particular, it is shown that a single-leader network is controllable if it is a directed path or cycle, but it is uncontrollable for a complete digraph or a star digraph in general. Furthermore, some approaches to improving the controllability of a heterogenous network are presented. Some simulation examples are given for illustration and verification.

  13. Model-based dynamic control and optimization of gas networks

    Energy Technology Data Exchange (ETDEWEB)

    Hofsten, Kai

    2001-07-01

    This work contributes to the research on control, optimization and simulation of gas transmission systems to support the dispatch personnel at gas control centres for the decision makings in the daily operation of the natural gas transportation systems. Different control and optimization strategies have been studied. The focus is on the operation of long distance natural gas transportation systems. Stationary optimization in conjunction with linear model predictive control using state space models is proposed for supply security, the control of quality parameters and minimization of transportation costs for networks offering transportation services. The result from the stationary optimization together with a reformulation of a simplified fluid flow model formulates a linear dynamic optimization model. This model is used in a finite time control and state constrained linear model predictive controller. The deviation from the control and the state reference determined from the stationary optimization is penalized quadratically. Because of the time varying status of infrastructure, the control space is also generally time varying. When the average load is expected to change considerably, a new stationary optimization is performed, giving a new state and control reference together with a new dynamic model that is used for both optimization and state estimation. Another proposed control strategy is a control and output constrained nonlinear model predictive controller for the operation of gas transmission systems. Here, the objective is also the security of the supply, quality control and minimization of transportation costs. An output vector is defined, which together with a control vector are both penalized quadratically from their respective references in the objective function. The nonlinear model predictive controller can be combined with a stationary optimization. At each sampling instant, a non convex nonlinear programming problem is solved giving a local minimum

  14. A geometrical approach to control and controllability of nonlinear dynamical networks.

    Science.gov (United States)

    Wang, Le-Zhi; Su, Ri-Qi; Huang, Zi-Gang; Wang, Xiao; Wang, Wen-Xu; Grebogi, Celso; Lai, Ying-Cheng

    2016-04-14

    In spite of the recent interest and advances in linear controllability of complex networks, controlling nonlinear network dynamics remains an outstanding problem. Here we develop an experimentally feasible control framework for nonlinear dynamical networks that exhibit multistability. The control objective is to apply parameter perturbation to drive the system from one attractor to another, assuming that the former is undesired and the latter is desired. To make our framework practically meaningful, we consider restricted parameter perturbation by imposing two constraints: it must be experimentally realizable and applied only temporarily. We introduce the concept of attractor network, which allows us to formulate a quantifiable controllability framework for nonlinear dynamical networks: a network is more controllable if the attractor network is more strongly connected. We test our control framework using examples from various models of experimental gene regulatory networks and demonstrate the beneficial role of noise in facilitating control.

  15. Protein dynamics in individual human cells: experiment and theory.

    Directory of Open Access Journals (Sweden)

    Ariel Aharon Cohen

    Full Text Available A current challenge in biology is to understand the dynamics of protein circuits in living human cells. Can one define and test equations for the dynamics and variability of a protein over time? Here, we address this experimentally and theoretically, by means of accurate time-resolved measurements of endogenously tagged proteins in individual human cells. As a model system, we choose three stable proteins displaying cell-cycle-dependant dynamics. We find that protein accumulation with time per cell is quadratic for proteins with long mRNA life times and approximately linear for a protein with short mRNA lifetime. Both behaviors correspond to a classical model of transcription and translation. A stochastic model, in which genes slowly switch between ON and OFF states, captures measured cell-cell variability. The data suggests, in accordance with the model, that switching to the gene ON state is exponentially distributed and that the cell-cell distribution of protein levels can be approximated by a Gamma distribution throughout the cell cycle. These results suggest that relatively simple models may describe protein dynamics in individual human cells.

  16. Switch Control between Different Speeds for a Passive Dynamic Walker

    Directory of Open Access Journals (Sweden)

    Limei Liu

    2012-12-01

    Full Text Available To make a biped robot walk stably at various speeds, a novel switch control approach is proposed to make the gaits switch smoothly between different walking speeds. The switch controller is designed based on the Lyapunov stability theory and the sufficient condition is given to make the closed-loop system stable. This controller can allow the robot to reach the stable gaits corresponding to the various speeds and improve the robustness of switch process. Potential energy compensation control has been studied in the dynamic model of a passive dynamic walking robot with knees. The functional relationship between the initial states and the walking speed is obtained. Numerical simulations are provided to verify the effectiveness of the control strategy.

  17. Switch Control Between Different Speeds for a Passive Dynamic Walker

    Directory of Open Access Journals (Sweden)

    Limei Liu

    2012-12-01

    Full Text Available To make a biped robot walk stably at various speeds, a novel switch control approach is proposed to make the gaits switch smoothly between different walking speeds. The switch controller is designed based on the Lyapunov stability theory and the sufficient condition is given to make the closed‐loop system stable. This controller can allow the robot to reach the stable gaits corresponding to the various speeds and improve the robustness of switch process. Potential energy compensation control has been studied in the dynamic model of a passive dynamic walking robot with knees. The functional relationship between the initial states and the walking speed is obtained. Numerical simulations are provided to verify the effectiveness of the control strategy.

  18. Z Number Based Fuzzy Inference System for Dynamic Plant Control

    Directory of Open Access Journals (Sweden)

    Rahib H. Abiyev

    2016-01-01

    Full Text Available Frequently the reliabilities of the linguistic values of the variables in the rule base are becoming important in the modeling of fuzzy systems. Taking into consideration the reliability degree of the fuzzy values of variables of the rules the design of inference mechanism acquires importance. For this purpose, Z number based fuzzy rules that include constraint and reliability degrees of information are constructed. Fuzzy rule interpolation is presented for designing of an inference engine of fuzzy rule-based system. The mathematical background of the fuzzy inference system based on interpolative mechanism is developed. Based on interpolative inference process Z number based fuzzy controller for control of dynamic plant has been designed. The transient response characteristic of designed controller is compared with the transient response characteristic of the conventional fuzzy controller. The obtained comparative results demonstrate the suitability of designed system in control of dynamic plants.

  19. Dynamics and Control of Adaptive Shells with Curvature Transformations

    Directory of Open Access Journals (Sweden)

    H.S. Tzou

    1995-01-01

    Full Text Available Adaptive structures with controllable geometries and shapes are rather useful in many engineering applications, such as adaptive wings, variable focus mirrors, adaptive machines, micro-electromechanical systems, etc. Dynamics and feedback control effectiveness of adaptive shells whose curvatures are actively controlled and continuously changed are evaluated. An adaptive piezoelectric laminated cylindrical shell composite with continuous curvature changes is studied, and its natural frequencies and controlled damping ratios are evaluated. The curvature change of the adaptive shell starts from an open shallow shell (30° and ends with a deep cylindrical shell (360°. Dynamic characteristics and control effectiveness (via the proportional velocity feedback of this series of shells are investigated and compared at every 30° curvature change. Analytical solutions suggest that the lower modes are sensitive to curvature changes and the higher modes are relatively insensitive.

  20. Improvements and applications of entrainment control for nonlinear dynamical systems.

    Science.gov (United States)

    Liu, Fang; Song, Qiang; Cao, Jinde

    2008-12-01

    This paper improves the existing entrainment control approaches and develops unified schemes to chaos control and generalized (lag, anticipated, and complete) synchronization of nonlinear dynamical systems. By introducing impulsive effects to the open-loop control method, we completely remove its restrictions on goal dynamics and initial conditions, and derive a sufficient condition to estimate the upper bound of impulsive intervals to ensure the global asymptotic stability. We then propose two effective ways to implement the entrainment strategy which combine open-loop and closed-loop control, and we prove that the feedback gains can be chosen according to a lower bound or be tuned with an adaptive control law. Numerical examples are given to verify the theoretical results and to illustrate their applications.

  1. Photosensitive chitosan to control cell attachment.

    Science.gov (United States)

    Cheng, Nan; Cao, Xudong

    2011-09-01

    An approach to control cell adhesion using a photocleavable molecule on chitosan has been developed and studied. Photocleavable 4,5-dimethoxy-2-nitrobenzyl chloroformate (NVOC) was introduced into chitosan to control the surface properties. The two UV illuminations with a photomask controlled the cleavage of NVOC and the presentation of deprotected amines on one chitosan surface spatially and temporally. The following immobilizations of cell repulsive poly(ethylene glycol) after the first illumination and cell adhesive sequence Arg-Gly-Asp-Ser (RGDS) after the second illumination on the surface helped create surface heterogeneity. Fourier transform infrared spectroscopy (FTIR), water contact angle, and UV-visible spectroscopy were used to characterize the surfaces and photoactivation during the process. To study the cell attachment and morphology on our designed surfaces, NIH/3T3 fibroblast cell was used. Cell number and morphology on the surfaces were investigated. The cell study demonstrated the feasibility of the surfaces on the control of cell adhesion and the formation of cell patterns by UV illuminations and the following immobilizations of different biomolecules. Crown Copyright © 2011. Published by Elsevier Inc. All rights reserved.

  2. Dynamics of cell degradation. [nickel cadmium batteries

    Science.gov (United States)

    Mcdermott, P. P.

    1978-01-01

    The use of chemical and physical data as a supplement to linear regression models in the prediction of cell failure is discussed. Principal factors to be considered are the positive thickness and weight, and the negative thickness. A model for cell degradation and failure in accelerated life test cells is presented and predictions based on a teardown analysis are included.

  3. Multi-Scale Dynamics, Control, and Simulation of Granular Spacecraft

    Science.gov (United States)

    Quadrelli, Marco B.; Basinger, Scott; Swartzlander, Grover

    2013-01-01

    In this paper, we present some ideas regarding the modeling, dynamics and control aspects of granular spacecraft. Granular spacecraft are complex multibody systems composed of a spatially disordered distribution of a large number of elements, for instance a cloud of grains in orbit. An example of application is a spaceborne observatory for exoplanet imaging, where the primary aperture is a cloud instead of a monolithic aperture. A model is proposed of a multi-scale dynamics of the grains and cloud in orbit, as well as a control approach for cloud shape maintenance and alignment, and preliminary simulation studies are carried out for the representative imaging system.

  4. Linear dynamical quantum systems analysis, synthesis, and control

    CERN Document Server

    Nurdin, Hendra I

    2017-01-01

    This monograph provides an in-depth treatment of the class of linear-dynamical quantum systems. The monograph presents a detailed account of the mathematical modeling of these systems using linear algebra and quantum stochastic calculus as the main tools for a treatment that emphasizes a system-theoretic point of view and the control-theoretic formulations of quantum versions of familiar problems from the classical (non-quantum) setting, including estimation and filtering, realization theory, and feedback control. Both measurement-based feedback control (i.e., feedback control by a classical system involving a continuous-time measurement process) and coherent feedback control (i.e., feedback control by another quantum system without the intervention of any measurements in the feedback loop) are treated. Researchers and graduates studying systems and control theory, quantum probability and stochastics or stochastic control whether from backgrounds in mechanical or electrical engineering or applied mathematics ...

  5. Nonlinear systems techniques for dynamical analysis and control

    CERN Document Server

    Lefeber, Erjen; Arteaga, Ines

    2017-01-01

    This treatment of modern topics related to the control of nonlinear systems is a collection of contributions celebrating the work of Professor Henk Nijmeijer and honoring his 60th birthday. It addresses several topics that have been the core of Professor Nijmeijer’s work, namely: the control of nonlinear systems, geometric control theory, synchronization, coordinated control, convergent systems and the control of underactuated systems. The book presents recent advances in these areas, contributed by leading international researchers in systems and control. In addition to the theoretical questions treated in the text, particular attention is paid to a number of applications including (mobile) robotics, marine vehicles, neural dynamics and mechanical systems generally. This volume provides a broad picture of the analysis and control of nonlinear systems for scientists and engineers with an interest in the interdisciplinary field of systems and control theory. The reader will benefit from the expert participan...

  6. Controlled surface chemistries and quantitative cell response

    Science.gov (United States)

    Plant, Anne L.

    2002-03-01

    Living cells experience a large number of signaling cues from their extracellular matrix. As a result of these inputs, a variety of intracellular signaling pathways are apparently initiated simultaneously. The vast array of alternative responses that result from the integration of these inputs suggests that it may be reasonable to look for cellular response not as an 'on' or 'off' condition but as a distribution of responses. A difficult challenge is to determine whether variations in responses from individual cells arise from the complexity of intracellular signals or are due to variations in the cell culture environment. By controlling surface chemistry so that every cell 'sees' the same chemical and physical environment, we can begin to assess how the distribution of cell response is affected strictly by changes in the chemistry of the cell culture surface. Using the gene for green fluorescent protein linked to the gene for the promoter of the extracellular matrix protein, tenascin, we can easily probe the end product in a signaling pathway that is purported to be linked to surface protein chemistry and to cell shape. Cell response to well-controlled, well-characterized, and highly reproducible surfaces prepared using soft lithography techniques are compared with more conventional ways of preparing extracellular matrix proteins for cell culture. Using fluorescence microscopy and image analysis of populations of cells on these surfaces, we probe quantitatively the relationship between surface chemistry, cell shape and variations in gene expression endpoint.

  7. Dynamics and Control of a Flexible Solar Sail

    Directory of Open Access Journals (Sweden)

    Jiafu Liu

    2014-01-01

    Full Text Available Solar sail can merely make use of solar radiation pressure (SRP force as the thrust for space missions. The attitude dynamics is obtained for the highly flexible solar sail with control vanes, sliding masses, and a gimbaled control boom. The vibration equations are derived considering the geometric nonlinearity of the sail structure subjected to the forces generated by the control vanes, solar radiation pressure (SRP, and sliding masses. Then the dynamic models for attitude/vibration controller design and dynamic simulation are obtained, respectively. The linear quadratic regulator (LQR based and optimal proportional-integral (PI based controllers are designed for the coupled attitude/vibration models with constant disturbance torques caused by the center-of-mass (cm/center-of-pressure (cp offset, respectively. It can be concluded from the theoretical analysis and simulation results that the optimal PI based controller performs better than the LQR based controller from the view of eliminating the steady-state errors. The responses with and without the geometrical nonlinearity are performed, and the differences are observed and analyzed. And some suggestions are also presented.

  8. Robust adaptive control of nonlinearly parameterized systems with unmodeled dynamics

    Institute of Scientific and Technical Information of China (English)

    LIU Yu-sheng; CHEN Jiang; LI Xing-yuan

    2006-01-01

    Many physical systems such as biochemical processes and machines with friction are of nonlinearly parameterized systems with uncertainties.How to control such systems effectively is one of the most challenging problems.This paper presents a robust adaptive controller for a significant class of nonlinearly parameterized systems.The controller can be used in cases where there exist parameter and nonlinear uncertainties,unmodeled dynamics and unknown bounded disturbances.The design of the controller is based on the control Lyapunov function method.A dynamic signal is introduced and adaptive nonlinear damping terms are used to restrain the effects of unmodeled dynamics,nonlinear uncertainties and unknown bounded disturbances.The backstepping procedure is employed to overcome the complexity in the design.With the proposed method,the estimation of the unknown parameters of the system is not required and there is only one adaptive parameter no matter how high the order of the system is and how many unknown parameters.there are.It is proved theoretically that the proposed robust adaptive control scheme guarantees the stability of nonlinearly parameterized system.Furthermore,all the states approach the equilibrium in arbitrary precision by choosing some design constants appropriately.Simulation results illustrate the effectiveness of the proposed robust adaptive controller.

  9. Matrix expression and vaccination control for epidemic dynamics over dynamic networks

    Institute of Scientific and Technical Information of China (English)

    Peilian GUO; Yuzhen WANG

    2016-01-01

    This paper investigates epidemic dynamics over dynamic networks via the approach of semi-tensor product of matrices. First, a formal susceptible-infected-susceptible epidemic dynamic model over dynamic networks (SISED-DN) is given. Second, based on a class of determinate co-evolutionary rule, the matrix expressions are established for the dynamics of individual states and network topologies, respectively. Then, all possible final spreading equilibria are obtained for any given initial epidemic state and network topology by the matrix expression. Third, a sufficient and necessary condition of the existence of state feedback vaccination control is presented to make every individual susceptible. The study of illustrative examples shows the effectiveness of our new results.

  10. Infrared-Controlled Welding of Solar Cells

    Science.gov (United States)

    Paulson, R.; Finnell, S. E.; Decker, H. J.; Hodor, J. R.

    1982-01-01

    Proposed apparatus for welding large arrays of solar cells to flexible circuit substrates would sense infrared emission from welding spot. Emission would provide feedback for control of welding heat. Welding platform containing optical fibers moves upward through slots in movable holding fixture to contact solar cells. Fibers pick up infrared radiation from weld area.

  11. Cell shape dynamics: from waves to migration.

    Directory of Open Access Journals (Sweden)

    Meghan K Driscoll

    Full Text Available We observe and quantify wave-like characteristics of amoeboid migration. Using the amoeba Dictyostelium discoideum, a model system for the study of chemotaxis, we demonstrate that cell shape changes in a wave-like manner. Cells have regions of high boundary curvature that propagate from the leading edge toward the back, usually along alternating sides of the cell. Curvature waves are easily seen in cells that do not adhere to a surface, such as cells that are electrostatically repelled from surfaces or cells that extend over the edge of micro-fabricated cliffs. Without surface contact, curvature waves travel from the leading edge to the back of a cell at -35 µm/min. Non-adherent myosin II null cells do not exhibit these curvature waves. At the leading edge of adherent cells, curvature waves are associated with protrusive activity. Like regions of high curvature, protrusive activity travels along the boundary in a wave-like manner. Upon contact with a surface, the protrusions stop moving relative to the surface, and the boundary shape thus reflects the history of protrusive motion. The wave-like character of protrusions provides a plausible mechanism for the zig-zagging of pseudopods and for the ability of cells both to swim in viscous fluids and to navigate complex three dimensional topography.

  12. Delaunay-Object-Dynamics: cell mechanics with a 3D kinetic and dynamic weighted Delaunay-triangulation.

    Science.gov (United States)

    Meyer-Hermann, Michael

    2008-01-01

    Mathematical methods in Biology are of increasing relevance for understanding the control and the dynamics of biological systems with medical relevance. In particular, agent-based methods turn more and more important because of fast increasing computational power which makes even large systems accessible. An overview of different mathematical methods used in Theoretical Biology is provided and a novel agent-based method for cell mechanics based on Delaunay-triangulations and Voronoi-tessellations is explained in more detail: The Delaunay-Object-Dynamics method. It is claimed that the model combines physically realistic cell mechanics with a reasonable computational load. The power of the approach is illustrated with two examples, avascular tumor growth and genesis of lymphoid tissue in a cell-flow equilibrium.

  13. Dynamics of spacecraft control laboratory experiment (SCOLE) slew maneuvers

    Science.gov (United States)

    Kakad, Y. P.

    1987-01-01

    This is the first of two reports on the dynamics and control of slewing maneuvers of the NASA Spacecraft Control Laboratory Experiment (SCOLE). In this report, the dynamics of slewing maneuvers of SCOLE are developed in terms of an arbitrary maneuver about any given axis. The set of dynamical equations incorporate rigid-body slew maneuver and three-dimensional vibrations of the complete assembly comprising the rigid shuttle, the flexible beam, and the reflector with an offset mass. The analysis also includes kinematic nonlinearities of the entire assembly during the maneuver and the dynamics of the interaction between the rigid shuttle and the flexible appendage. The final set of dynamical equations obtained for slewing maneuvers is highly nonlinear and coupled in terms of the flexible modes and the rigid-body modes. The equations are further simplified and evaluated numerically to include the first ten flexible modes and the SCOLE data to yield a model for designing control systems to perform slew maneuvers.

  14. Generation and precise control of dynamic biochemical gradients for cellular assays

    CERN Document Server

    Saka, Yasushi; Giuraniuc, Claudiu V

    2016-01-01

    Spatial gradients of diffusible signalling molecules play crucial roles in controlling diverse cellular behaviour such as cell differentiation, tissue patterning and chemotaxis. Here we present a microfluidic platform for cellular assays that can generate and control diffusion-based gradients dynamically. A unique design of the device eliminates cross-flow between the source and sink channels, thereby stabilising gradients by passive diffusion. The platform also enables quick and flexible control of chemical concentration that makes highly dynamic gradients in diffusion chambers. Budding yeast cells cultured in a gradient of a chemical inducer expressed a reporter fluorescence protein in a concentration-dependent manner. This microfluidic platform serves as a versatile prototype applicable to a broad range of biomedical investigations.

  15. Balancing Control Strategy for Li-Ion Batteries String Based on Dynamic Balanced Point

    Directory of Open Access Journals (Sweden)

    Dong-Hua Zhang

    2015-03-01

    Full Text Available The Li-ion battery is becoming the optimal choice for the Electric Vehicle’s (EV power supply. In order to protect the Li-ion battery from charging damage and to prolong the battery’s life, a special control strategy based on the dynamic balanced point along with a non-dissipative equalizer is presented. The main focus of the proposed control strategy is to insure that the individual cell of a battery pack will be rapidly, efficiently and simultaneously balanced, by adjusting equalizing current of each cell dynamically. In this paper, a model of a four series connected Li-ion batteries pack has been established to evaluate the proposed control strategy. Superior performance is demonstrated by the simulation and experiment.

  16. Control uncertain continuous-time chaotic dynamical system

    Institute of Scientific and Technical Information of China (English)

    齐冬莲; 赵光宙

    2003-01-01

    The new chaos control method presented in this paper is useful for taking advantage of chaos.Based on sliding mode control theory, this paper provides a switching manifold controlling strategy of chaoticsystem, and also gives a kind of adaptive parameters estimated method to estimate the unknown systems' pa-rameters by which chaotic dynamical system can be synchronized. Taking the Lorenz system as example, and with the help of this controlling strategy, we can synchronize chaotic systems with unknown parameters and different initial conditions.

  17. Flight Dynamics and Control of Elastic Hypersonic Vehicles Uncertainty Modeling

    Science.gov (United States)

    Chavez, Frank R.; Schmidt, David K.

    1994-01-01

    It has been shown previously that hypersonic air-breathing aircraft exhibit strong aeroelastic/aeropropulsive dynamic interactions. To investigate these, especially from the perspective of the vehicle dynamics and control, analytical expressions for key stability derivatives were derived, and an analysis of the dynamics was performed. In this paper, the important issue of model uncertainty, and the appropriate forms for representing this uncertainty, is addressed. It is shown that the methods suggested in the literature for analyzing the robustness of multivariable feedback systems, which as a prerequisite to their application assume particular forms of model uncertainty, can be difficult to apply on real atmospheric flight vehicles. Also, the extent to which available methods are conservative is demonstrated for this class of vehicle dynamics.

  18. Controlled Activation of Protein Rotational Dynamics Using Smart Hydrogel Tethering

    Energy Technology Data Exchange (ETDEWEB)

    Beech, Brenda M.; Xiong, Yijia; Boschek, Curt B.; Baird, Cheryl L.; Bigelow, Diana J.; Mcateer, Kathleen; Squier, Thomas C.

    2014-09-05

    Stimulus-responsive hydrogel materials that stabilize and control protein dynamics have the potential to enable a range of applications to take advantage of the inherent specificity and catalytic efficiencies of proteins. Here we describe the modular construction of a hydrogel using an engineered calmodulin (CaM) within a polyethylene glycol (PEG) matrix that involves the reversible tethering of proteins through an engineered CaM-binding sequence. For these measurements, maltose binding protein (MBP) was isotopically labeled with [13C] and [15N], permitting dynamic structural measurements using TROSY-HSQC NMR spectroscopy. Upon initial formation of hydrogels protein dynamics are suppressed, with concomitant increases in protein stability. Relaxation of the hydrogel matrix following transient heating results in the activation of protein dynamics and restoration of substrate-induced large-amplitude domain motions necessary for substrate binding.

  19. TOG Proteins Are Spatially Regulated by Rac-GSK3β to Control Interphase Microtubule Dynamics.

    Directory of Open Access Journals (Sweden)

    Kathryn P Trogden

    Full Text Available Microtubules are regulated by a diverse set of proteins that localize to microtubule plus ends (+TIPs where they regulate dynamic instability and mediate interactions with the cell cortex, actin filaments, and organelles. Although individual +TIPs have been studied in depth and we understand their basic contributions to microtubule dynamics, there is a growing body of evidence that these proteins exhibit cross-talk and likely function to collectively integrate microtubule behavior and upstream signaling pathways. In this study, we have identified a novel protein-protein interaction between the XMAP215 homologue in Drosophila, Mini spindles (Msps, and the CLASP homologue, Orbit. These proteins have been shown to promote and suppress microtubule dynamics, respectively. We show that microtubule dynamics are regionally controlled in cells by Rac acting to suppress GSK3β in the peripheral lamellae/lamellipodium. Phosphorylation of Orbit by GSK3β triggers a relocalization of Msps from the microtubule plus end to the lattice. Mutation of the Msps-Orbit binding site revealed that this interaction is required for regulating microtubule dynamic instability in the cell periphery. Based on our findings, we propose that Msps is a novel Rac effector that acts, in partnership with Orbit, to regionally regulate microtubule dynamics.

  20. Relevant parameters in models of cell division control

    CERN Document Server

    Grilli, Jacopo; Kennard, Andrew S; Lagomarsino, Marco Cosentino

    2016-01-01

    A recent burst of dynamic single-cell growth-division data makes it possible to characterize the stochastic dynamics of cell division control in bacteria. Different modeling frameworks were used to infer specific mechanisms from such data, but the links between frameworks are poorly explored, with relevant consequences for how well any particular mechanism can be supported by the data. Here, we describe a simple and generic framework in which two common formalisms can be used interchangeably: (i) a continuous-time division process described by a hazard function and (ii) a discrete-time equation describing cell size across generations (where the unit of time is a cell cycle). In our framework, this second process is a discrete-time Langevin equation with a simple physical analogue. By perturbative expansion around the mean initial size (or inter-division time), we show explicitly how this framework describes a wide range of division control mechanisms, including combinations of time and size control, as well a...

  1. On Controllability and Observability of Fuzzy Dynamical Matrix Lyapunov Systems

    Directory of Open Access Journals (Sweden)

    M. S. N. Murty

    2008-04-01

    Full Text Available We provide a way to combine matrix Lyapunov systems with fuzzy rules to form a new fuzzy system called fuzzy dynamical matrix Lyapunov system, which can be regarded as a new approach to intelligent control. First, we study the controllability property of the fuzzy dynamical matrix Lyapunov system and provide a sufficient condition for its controllability with the use of fuzzy rule base. The significance of our result is that given a deterministic system and a fuzzy state with rule base, we can determine the rule base for the control. Further, we discuss the concept of observability and give a sufficient condition for the system to be observable. The advantage of our result is that we can determine the rule base for the initial value without solving the system.

  2. CONTROLLABILITY OF ESSENTIALLY VARIOUS-SPEED SINGULARLY PERTURBED DYNAMIC SYSTEMS

    Directory of Open Access Journals (Sweden)

    T. Kopeikina

    2013-01-01

    Full Text Available The paper considers the controllability problem of essentially various-speed singularly perturbed dynamic system consisting of three subsystems of different dimensions, containing a small parameter to a variable degree as a multiplier for derivatives.   A method for studying complete and relative controllability of such systems has been proposed in the paper. The method is based on investigation of a controllability matrix rank. The matrix is composed of solution components of algebraic recurrent equations, which are drawn directly in accordance with the studied system of differential equations. The obtained effective algebraic conditions of controllability, expressed through parameters of the investigated system are obtained are illustrated by the case of essentially various-speed singularly perturbed dynamic system of fifth order with rational powers of small parameter.

  3. Survey of orbital dynamics and control of space rendezvous

    Institute of Scientific and Technical Information of China (English)

    Luo Yazhong; Zhang Jin; Tang Guojin

    2014-01-01

    Rendezvous orbital dynamics and control (RODC) is a key technology for operating space rendezvous and docking missions. This paper surveys the studies on RODC. Firstly, the basic relative dynamics equation set is introduced and its improved versions are evaluated. Secondly, studies on rendezvous trajectory optimization are commented from three aspects:the linear rendez-vous, the nonlinear two-body rendezvous, and the perturbed and constrained rendezvous. Thirdly, studies on relative navigation are briefly reviewed, and then close-range control methods including automated control, manual control, and telecontrol are analyzed. Fourthly, advances in rendezvous trajectory safety and robust analysis are surveyed, and their applications in trajectory optimization are discussed. Finally, conclusions are drawn and prospects of studies on RODC are presented.

  4. Cascaded adaptive control of ocean vehicles with significant actuator dynamics

    Directory of Open Access Journals (Sweden)

    Thor I. Fossen

    1994-04-01

    Full Text Available This paper presents a cascade adaptive control scheme for marine vehicles where the non-linear equations of motion include a model of the actuator dynamics. The adaptive controller does not require the parameters of the vehicle dynamics and the actuator time constants to be known a priori. Both the velocity and position tracking errors are shown to converge to zero by applying Barbalat's lemma. Global asymptotic stability is proven for the velocity scheme while the position/attitude controller is only proven to be convergent. Furthermore, all parameter estimates are shown to be bounded. Computer simulations of an ROV speed control system and an autopilot for automatic ship steering are used to illustrate the design methodology.

  5. Local Nucleosome Dynamics Facilitate Chromatin Accessibility in Living Mammalian Cells

    Directory of Open Access Journals (Sweden)

    Saera Hihara

    2012-12-01

    Full Text Available Genome information, which is three-dimensionally organized within cells as chromatin, is searched and read by various proteins for diverse cell functions. Although how the protein factors find their targets remains unclear, the dynamic and flexible nature of chromatin is likely crucial. Using a combined approach of fluorescence correlation spectroscopy, single-nucleosome imaging, and Monte Carlo computer simulations, we demonstrate local chromatin dynamics in living mammalian cells. We show that similar to interphase chromatin, dense mitotic chromosomes also have considerable chromatin accessibility. For both interphase and mitotic chromatin, we observed local fluctuation of individual nucleosomes (∼50 nm movement/30 ms, which is caused by confined Brownian motion. Inhibition of these local dynamics by crosslinking impaired accessibility in the dense chromatin regions. Our findings show that local nucleosome dynamics drive chromatin accessibility. We propose that this local nucleosome fluctuation is the basis for scanning genome information.

  6. Randomized Dynamical Decoupling Techniques for Coherent Quantum Control

    CERN Document Server

    Viola, L; Viola, Lorenza; Santos, Lea F.

    2006-01-01

    The need for strategies able to accurately manipulate quantum dynamics is ubiquitous in quantum control and quantum information processing. We investigate two scenarios where randomized dynamical decoupling techniques become more advantageous with respect to standard deterministic methods in switching off unwanted dynamical evolution in a closed quantum system: when dealing with decoupling cycles which involve a large number of control actions and/or when seeking long-time quantum information storage. Highly effective hybrid decoupling schemes, which combine deterministic and stochastic features are discussed, as well as the benefits of sequentially implementing a concatenated method, applied at short times, followed by a hybrid protocol, employed at longer times. A quantum register consisting of a chain of spin-1/2 particles interacting via the Heisenberg interaction is used as a model for the analysis throughout.

  7. Two-mode dynamics in pulse-modulated control systems

    DEFF Research Database (Denmark)

    Zhusubaliyev, Z.T.; Yanochkina, O.O.; Mosekilde, Erik

    2010-01-01

    as an example the paper provides a survey of three new mechanisms of torus bifurcation that can be observed in pulse-modulated control systems. The paper concludes with a discussion of the influence that operation in the torus regimes will have on the efficiency of the converter......Pulse-modulated converter systems play an important role in modern power electronics. Systems of this type also deserve considerable theoretical interest because of the complex interplay they exhibit between ordinary (smooth) bifurcations and so-called border-collision bifurcations generated...... by the switching dynamics. Particularly interesting are the unusual transitions to torus dynamics, i.e., to a mode of behavior in which the regular switching dynamics is modulated by another oscillatory mode that may arise through instability in the feedback control. Using the model of a two-level DC/DC converter...

  8. Predictive control and identification: Applications to steering dynamics

    DEFF Research Database (Denmark)

    Hansen, Anca Daniela

    1996-01-01

    The main objective of the present thesis is to enhance insight into predictive controller design and identification in connection with steering dynamics. In Chapter 2, the dynamics of ship steering are reviewed. Models of different complexity, suitable for control systems design are presented....... The influence of wind, waves and currents on the chip motions are also discussed. Chapter 3 deals with the model reduction problem. Some basic concepts are explained, due to their role in the reduction of the dynamic models. Two model reductions techniques, based on singular values, are described....... The theoretical properties of these methods are studied and their performance is examined via simulation on a stochastic linear Mariner Class Vessel model. In Chapter 4, the attention is focused on the derivation of an extended GPC. This extended strategy implies a generalization of the model structure...

  9. Controllability, observability, realizability, and stability of dynamic linear systems

    OpenAIRE

    Davis, John M.; Gravagne, Ian A.; Jackson, Billy J.; Marks II, Robert J.

    2009-01-01

    We develop a linear systems theory that coincides with the existing theories for continuous and discrete dynamical systems, but that also extends to linear systems defined on nonuniform time scales. The approach here is based on generalized Laplace transform methods (e.g. shifts and convolution) from the recent work [13]. We study controllability in terms of the controllability Gramian and various rank conditions (including Kalman's) in both the time invariant and time varying settings...

  10. Optimal control of stochastic magnetization dynamics by spin current

    Science.gov (United States)

    Wang, Yong; Zhang, Fu-Chun

    2013-05-01

    Fluctuation-induced stochastic magnetization dynamics plays an important role in spintronics devices. Here we propose that it can be optimally controlled by spin currents to minimize or maximize the Freidlin-Wentzell action functional of the system hence to increase or decrease the probability of the large fluctuations. We apply this method to study the thermally activated magnetization switching problem and to demonstrate the merits of the optimal control strategy.

  11. A dynamic epidemic control model on uncorrelated complex networks

    Institute of Scientific and Technical Information of China (English)

    Pei Wei-Dong; Chen Zeng-Qiang; Yuan Zhu-Zhi

    2008-01-01

    In this paper,a dynamic epidemic control model on the uncorrelated complex networks is proposed.By means of theoretical analysis,we found that the new model has a similar epidemic threshold as that of the susceptible-infectedrecovered (SIR) model on the above networks,but it can reduce the prevalence of the infected individuals remarkably.This result may help us understand epidemic spreading phenomena on real networks and design appropriate strategies to control infections.

  12. Quantum Sensing of Noisy and Complex Systems under Dynamical Control

    Directory of Open Access Journals (Sweden)

    Gershon Kurizki

    2016-12-01

    Full Text Available We review our unified optimized approach to the dynamical control of quantum-probe interactions with noisy and complex systems viewed as thermal baths. We show that this control, in conjunction with tools of quantum estimation theory, may be used for inferring the spectral and spatial characteristics of such baths with high precision. This approach constitutes a new avenue in quantum sensing, dubbed quantum noise spectroscopy.

  13. Dynamics of cell polarity in tissue morphogenesis: a comparative view from Drosophila and Ciona [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Michael T. Veeman

    2016-06-01

    Full Text Available Tissues in developing embryos exhibit complex and dynamic rearrangements that shape forming organs, limbs, and body axes. Directed migration, mediolateral intercalation, lumen formation, and other rearrangements influence the topology and topography of developing tissues. These collective cell behaviors are distinct phenomena but all involve the fine-grained control of cell polarity. Here we review recent findings in the dynamics of polarized cell behavior in both the Drosophila ovarian border cells and the Ciona notochord. These studies reveal the remarkable reorganization of cell polarity during organ formation and underscore conserved mechanisms of developmental cell polarity including the Par/atypical protein kinase C (aPKC and planar cell polarity pathways. These two very different model systems demonstrate important commonalities but also key differences in how cell polarity is controlled in tissue morphogenesis. Together, these systems raise important, broader questions on how the developmental control of cell polarity contributes to morphogenesis of diverse tissues across the metazoa.

  14. Population dynamics and population control of Galium aparine L.

    NARCIS (Netherlands)

    Weide, van der R.Y.

    1993-01-01

    The population biology of Galium aparine L. needs to be better understood, in order to be able to rationalize decisions about the short- and long-term control of this weed species for different cropping practices.A population dynamics model was developed to simulate the basic processes of the life c

  15. Dynamically tuned magnetostrictive spring with electrically controlled stiffness

    Science.gov (United States)

    Scheidler, Justin J.; Asnani, Vivake M.; Dapino, Marcelo J.

    2016-03-01

    This paper presents the design and testing of an electrically controllable magnetostrictive spring that has a dynamically tunable stiffness (i.e., a magnetostrictive Varispring). The device enables in situ stiffness tuning or stiffness switching for vibration control applications. Using a nonlinear electromechanical transducer model and an analytical solution of linear, mechanically induced magnetic diffusion, Terfenol-D is shown to have a faster rise time to stepped voltage inputs and a significantly higher magnetic diffusion cut-off frequency relative to Galfenol. A Varispring is manufactured using a laminated Terfenol-D rod. Further rise time reductions are achieved by minimizing the rod’s diameter and winding the electromagnet with larger wire. Dynamic tuning of the Varispring’s stiffness is investigated by measuring the Terfenol-D rod’s strain response to dynamic, compressive, axial forces in the presence of sinusoidal or square wave control currents. The Varispring’s rise time is \\lt 1 ms for 1 A current switches. Continuous modulus changes up to 21.9 GPa and 500 Hz and square wave modulus changes (dynamic {{Δ }}E effect) up to 12.3 GPa and 100 Hz are observed. Stiffness tunability and tuning bandwidth can be considerably increased by operating about a more optimal bias stress and improving the control of the electrical input.

  16. Integrated vehicle dynamics control using State Dependent Riccati Equations

    NARCIS (Netherlands)

    Bonsen, B.; Mansvelders, R.; Vermeer, E.

    2010-01-01

    In this paper we discuss a State Dependent Riccati Equations (SDRE) solution for Integrated Vehicle Dynamics Control (IVDC). The SDRE approach is a nonlinear variant of the well known Linear Quadratic Regulator (LQR) and implements a quadratic cost function optimization. A modified version of this

  17. Dynamic self-assembly and control of microfluidic particle crystals

    Science.gov (United States)

    Lee, Wonhee; Amini, Hamed; Stone, Howard A.; Di Carlo, Dino

    2010-01-01

    Engineered two-phase microfluidic systems have recently shown promise for computation, encryption, and biological processing. For many of these systems, complex control of dispersed-phase frequency and switching is enabled by nonlinearities associated with interfacial stresses. Introducing nonlinearity associated with fluid inertia has recently been identified as an easy to implement strategy to control two-phase (solid-liquid) microscale flows. By taking advantage of inertial effects we demonstrate controllable self-assembling particle systems, uncover dynamics suggesting a unique mechanism of dynamic self-assembly, and establish a framework for engineering microfluidic structures with the possibility of spatial frequency filtering. Focusing on the dynamics of the particle–particle interactions reveals a mechanism for the dynamic self-assembly process; inertial lift forces and a parabolic flow field act together to stabilize interparticle spacings that otherwise would diverge to infinity due to viscous disturbance flows. The interplay of the repulsive viscous interaction and inertial lift also allow us to design and implement microfluidic structures that irreversibly change interparticle spacing, similar to a low-pass filter. Although often not considered at the microscale, nonlinearity due to inertia can provide a platform for high-throughput passive control of particle positions in all directions, which will be useful for applications in flow cytometry, tissue engineering, and metamaterial synthesis. PMID:21149674

  18. Graphene: A Dynamic Platform for Electrical Control of Plasmonic Resonance

    DEFF Research Database (Denmark)

    Emani, Naresh Kumar; Kildishev, Alexander V.; Shalaev, Vladimir M.

    2015-01-01

    Graphene has recently emerged as a viable platform for integrated optoelectronic and hybrid photonic devices because of its unique properties. The optical properties of graphene can be dynamically controlled by electrical voltage and have been used to modulate the plasmons in noble metal nanostru...

  19. General problems of dynamics and control of vibratory gyroscopes

    CSIR Research Space (South Africa)

    Shatalov, MY

    2008-05-01

    Full Text Available A general model of operation of vibratory gyroscopes, which is applicable to a broad class of instruments, including cylindrical, disc and micro-machined gyros, is formulated on the basis of analysis of dynamics and control of a hemispherical...

  20. Dynamic Positioning of Ships: A nonlinear control design study

    NARCIS (Netherlands)

    Muhammad, S.

    2012-01-01

    Dynamic positioning (DP) is relatively a new technique used to maintain the position and heading of ships in various offshore operations. Due to the features like better safety and operating efficiency, DP systems are becoming more and more popular. This thesis mainly focusses on the control system

  1. Integrated vehicle dynamics control using State Dependent Riccati Equations

    NARCIS (Netherlands)

    Bonsen, B.; Mansvelders, R.; Vermeer, E.

    2010-01-01

    In this paper we discuss a State Dependent Riccati Equations (SDRE) solution for Integrated Vehicle Dynamics Control (IVDC). The SDRE approach is a nonlinear variant of the well known Linear Quadratic Regulator (LQR) and implements a quadratic cost function optimization. A modified version of this m

  2. Optimal Estimator Synthesis for Ship Dynamic Object Control

    OpenAIRE

    BIYA MOTTO Frederic

    2014-01-01

    Optimal estimator synthesis algorithm for dynamic ship system control is considered. Estimator is constructed in the form of Kalman gain as steady-state Kalman filter. Estimator design is realised by MATLAB codes using account of wind gust noise with a set spectral intensity

  3. Integration of Predictive Routing Information with Dynamic Traffic Signal Control

    Science.gov (United States)

    1994-05-01

    vehicles without the on-board guidance aid (Harris, S., Rabone , A., et.al., 1992). The simulation developed was called ROute GUidance Simulation (ROGUS...Florida. Harris, S., Rabone , A., et.al. 1992. ROGUS: A Simulation of Dynamic Route Guidance Systems. Traffic Engineering and Control(33)327-329

  4. Model of the human controller of a dynamic system

    NARCIS (Netherlands)

    Wewerinke, P.H.

    1986-01-01

    In de last two decades considerable research effort has been devoted to the study of human involvement in dynamic systems. Especially human control behavior has been investigated, but in the restricted sense of continuous regulating against random disturbances, so as to minimize the system state dev

  5. Integrating Biological Systems in the Process Dynamics and Control Curriculum

    Science.gov (United States)

    Parker, Robert S.; Doyle, Francis J.; Henson, Michael A.

    2006-01-01

    The evolution of the chemical engineering discipline motivates a re-evaluation of the process dynamics and control curriculum. A key requirement of future courses will be the introduction of theoretical concepts and application examples relevant to emerging areas, notably complex biological systems. We outline the critical concepts required to…

  6. Fluids as Dynamic Templates for Cytoskeletal Proteins in Plant Cells

    CERN Document Server

    Lofthouse, J T

    2008-01-01

    The Dynamic Template model of biological cell membranes and the cytoplasm as spatially organised fluid layers is extended to plant cells, and is shown to offer a feasible shear driven mechanism for the co-alignment of internal and external fibres observed during growth and tropic responses

  7. Dynamically Authorized Role-Based Access Control for Grid Applications

    Institute of Scientific and Technical Information of China (English)

    YAO Hanbing; HU Heping; LU Zhengding; LI Ruixuan

    2006-01-01

    Grid computing is concerned with the sharing and coordinated use of diverse resources in distributed "virtual organizations". The heterogeneous, dynamic and multi-domain nature of these environments makes challenging security issues that demand new technical approaches. Despite the recent advances in access control approaches applicable to Grid computing, there remain issues that impede the development of effective access control models for Grid applications. Among them there are the lack of context-based models for access control, and reliance on identity or capability-based access control schemes. An access control scheme that resolve these issues is presented, and a dynamically authorized role-based access control (D-RBAC) model extending the RBAC with context constraints is proposed. The D-RABC mechanisms dynamically grant permissions to users based on a set of contextual information collected from the system and user's environments, while retaining the advantages of RBAC model. The implementation architecture of D-RBAC for the Grid application is also described.

  8. Walking Robots Dynamic Control Systems on an Uneven Terrain

    Directory of Open Access Journals (Sweden)

    MUNTEANU, M. S.

    2010-05-01

    Full Text Available The paper presents ZPM dynamic control of walking robots, developing an open architecture real time control multiprocessor system, in view of obtaining new capabilities for walking robots. The complexity of the movement mechanism of a walking robot was taken into account, being a repetitive tilting process with numerous instable movements and which can lead to its turnover on an uneven terrain. The control system architecture for the dynamic robot walking is presented in correlation with the control strategy which contains three main real time control loops: balance robot control using sensorial feedback, walking diagram control with periodic changes depending on the sensorial information during each walk cycle, predictable movement control based on a quick decision from the previous experimental data. The results obtained through simulation and experiments show an increase in mobility, stability in real conditions and obtaining of high performances related to the possibility of moving walking robots on terrains with a configuration as close as possible to real situations, respectively developing new technological capabilities of the walking robot control systems for slope movement and walking by overtaking or going around obstacles.

  9. Nonlinear Dynamics of Controlled Synchronizations of Manipulator System

    Directory of Open Access Journals (Sweden)

    Qingkai Han

    2014-01-01

    Full Text Available The nonlinear dynamics of the manipulator system which is controlled to achieve the synchronization motions is investigated in the paper. Firstly, the control strategies and modeling approaches of the manipulator system are given, in which the synchronization goal is defined by both synchronization errors and its derivatives. The synchronization controllers applied on the manipulator system include neuron synchronization controller, improved OPCL synchronization controller, and MRAC-PD synchronization controller. Then, an improved adaptive synchronized control strategy is proposed in order to estimate online the unknown structure parameters and state variables of the manipulator system and to realize the needed synchronous compensation. Furthermore, a robust adaptive synchronization controller is also researched to guarantee the dynamic stability of the system. Finally, the stability of motion synchronizations of the manipulator system possessing nonlinear component is discussed, together with the effect of control parameters and joint friction and others. Some typical motions such as motion bifurcations and the loss of synchronization of it are obtained and illustrated as periodic, multiperiodic, and/or chaotic motion patterns.

  10. Approximate Dynamic Programming in Tracking Control of a Robotic Manipulator

    Directory of Open Access Journals (Sweden)

    Marcin Szuster

    2016-02-01

    Full Text Available This article focuses on the implementation of an approximate dynamic programming algorithm in the discrete tracking control system of the three-degrees of freedom Scorbot-ER 4pc robotic manipulator. The controlled system is included in an articulated robots group which uses rotary joints to access their work space. The main part of the control system is a dual heuristic dynamic programming algorithm that consists of two structures designed in the form of neural networks: an actor and a critic. The actor generates the suboptimal control law while the critic approximates the difference of the value function from Bellman’s equation with respect to the state. The residual elements of the control system are the PD controller, the supervisory term and an additional control signal. The structure of the supervisory term derives from the stability analysis performed using the Lyapunov stability theorem. The control system works online, the neural networks’ weights-adaptation procedure is performed in every iteration step, and the neural networks’ preliminary learning process is not required. The performance of the control system was verified by a series of computer simulations and experiments performed using the Scorbot-ER 4pc robotic manipulator.

  11. Daple Coordinates Planar Polarized Microtubule Dynamics in Ependymal Cells and Contributes to Hydrocephalus

    Directory of Open Access Journals (Sweden)

    Maki Takagishi

    2017-07-01

    Full Text Available Motile cilia in ependymal cells, which line the cerebral ventricles, exhibit a coordinated beating motion that drives directional cerebrospinal fluid (CSF flow and guides neuroblast migration. At the apical cortex of these multi-ciliated cells, asymmetric localization of planar cell polarity (PCP proteins is required for the planar polarization of microtubule dynamics, which coordinates cilia orientation. Daple is a disheveled-associating protein that controls the non-canonical Wnt signaling pathway and cell motility. Here, we show that Daple-deficient mice present hydrocephalus and their ependymal cilia lack coordinated orientation. Daple regulates microtubule dynamics at the anterior side of ependymal cells, which in turn orients the cilial basal bodies required for the directional cerebrospinal fluid flow. These results demonstrate an important role for Daple in planar polarity in motile cilia and provide a framework for understanding the mechanisms and functions of planar polarization in the ependymal cells.

  12. Coupled Flow-Structure-Biochemistry Simulations of Dynamic Systems of Blood Cells Using an Adaptive Surface Tracking Method

    OpenAIRE

    Hoskins, M.H.; Kunz, R.F.; Bistline, J.E.; Dong, C.

    2009-01-01

    A method for the computation of low Reynolds number dynamic blood cell systems is presented. The specific system of interest here is interaction between cancer cells and white blood cells in an experimental flow system. Fluid dynamics, structural mechanics, six-degree-of freedom motion control and surface biochemistry analysis components are coupled in the context of adaptive octree-based grid generation. Analytical and numerical verification of the quasi-steady assumption for the fluid mecha...

  13. Signaling and Dynamic Actin Responses of B Cells on Topographical Substrates

    Science.gov (United States)

    Ketchum, Christina; Sun, Xiaoyu; Fourkas, John; Song, Wenxia; Upadhyaya, Arpita

    B cells become activated upon physical contact with antigen on the surface of antigen presenting cells, such as dendritic cells. Binding of the B cell receptor with antigen initiates actin-mediated spreading of B cells, signaling cascades and eventually infection fighting antibodies. Lymphocytes, including B cells and T cells, have been shown to be responsive to the physical parameters of the contact surface, such as antigen mobility and substrate stiffness. However the roll of surface topography on lymphocyte function is unknown. Here we investigate the degree to which substrate topography controls actin-mediated spreading and B cell activation using nano-fabricated surfaces and live cell imaging. The model topographical system consists of 600 nanometer tall ridges with spacing varying between 800 nanometers and 5 micrometers. Using TIRF imaging we observe actin dynamics, B cell receptor motion and calcium signaling of B cells as they spread on the ridged substrates. We show that the spacing between ridges had a strong effect on the dynamics of actin and calcium influx on B cells. Our results indicate that B cells are highly sensitive to surface topography during cell spreading and signaling activation.

  14. Cell Adhesion on Dynamic Supramolecular Surfaces Probed by Fluid Force Microscopy-Based Single-Cell Force Spectroscopy.

    Science.gov (United States)

    Sankaran, Shrikrishnan; Jaatinen, Leena; Brinkmann, Jenny; Zambelli, Tomaso; Vörös, Janos; Jonkheijm, Pascal

    2017-04-25

    Biomimetic and stimuli-responsive cell-material interfaces are actively being developed to study and control various cell-dynamics phenomena. Since cells naturally reside in the highly dynamic and complex environment of the extracellular matrix, attempts are being made to replicate these conditions in synthetic biomaterials. Supramolecular chemistry, dealing with noncovalent interactions, has recently provided possibilities to incorporate such dynamicity and responsiveness in various types of architectures. Using a cucurbit[8]uril-based host-guest system, we have successfully established a dynamic and electrochemically responsive interface for the display of the integrin-specific ligand, Arg-Gly-Asp (RGD), to promote cell adhesion. Due to the weak nature of the noncovalent forces by which the components at the interface are held together, we expected that cell adhesion would also be weaker in comparison to traditional interfaces where ligands are usually immobilized by covalent linkages. To assess the stability and limitations of our noncovalent interfaces, we performed single-cell force spectroscopy studies using fluid force microscopy. This technique enabled us to measure rupture forces of multiple cells that were allowed to adhere for several hours on individual substrates. We found that the rupture forces of cells adhered to both the noncovalent and covalent interfaces were nearly identical for up to several hours. We have analyzed and elucidated the reasons behind this result as a combination of factors including the weak rupture force between linear Arg-Gly-Asp and integrin, high surface density of the ligand, and increase in effective concentration of the supramolecular components under spread cells. These characteristics enable the construction of highly dynamic biointerfaces without compromising cell-adhesive properties.

  15. Macromolecular Dynamics in Red Blood Cells Investigated Using Neutron Spectroscopy

    CERN Document Server

    Stadler, Andreas Maximilian; Demmel, Franz; Artmann, Gerhard; 10.1098/rsif.2010.0306

    2011-01-01

    We present neutron scattering measurements on the dynamics of hemoglobin (Hb) in human red blood cells in vivo. Global and internal Hb dynamics were measured in the ps to ns time- and {\\AA} length-scale using quasielastic neutron backscattering spectroscopy. We observed the cross-over from global Hb short-time to long-time self-diffusion. Both short- and long-time diffusion coefficients agree quantitatively with predicted values from hydrodynamic theory of non-charged hard-sphere suspensions when a bound water fraction of around 0.23g H2O/ g Hb is taken into account. The higher amount of water in the cells facilitates internal protein fluctuations in the ps time-scale when compared to fully hydrated Hb powder. Slower internal dynamics of Hb in red blood cells in the ns time-range were found to be rather similar to results obtained with fully hydrated protein powders, solutions and E. coli cells.

  16. Relation Between the Cell Volume and the Cell Cycle Dynamics in Mammalian cell

    Science.gov (United States)

    Magno, A. C. G.; Oliveira, I. L.; Hauck, J. V. S.

    2016-08-01

    The main goal of this work is to add and analyze an equation that represents the volume in a dynamical model of the mammalian cell cycle proposed by Gérard and Goldbeter (2011) [1]. The cell division occurs when the cyclinB/Cdkl complex is totally degraded (Tyson and Novak, 2011)[2] and it reaches a minimum value. At this point, the cell is divided into two newborn daughter cells and each one will contain the half of the cytoplasmic content of the mother cell. The equations of our base model are only valid if the cell volume, where the reactions occur, is constant. Whether the cell volume is not constant, that is, the rate of change of its volume with respect to time is explicitly taken into account in the mathematical model, then the equations of the original model are no longer valid. Therefore, every equations were modified from the mass conservation principle for considering a volume that changes with time. Through this approach, the cell volume affects all model variables. Two different dynamic simulation methods were accomplished: deterministic and stochastic. In the stochastic simulation, the volume affects every model's parameters which have molar unit, whereas in the deterministic one, it is incorporated into the differential equations. In deterministic simulation, the biochemical species may be in concentration units, while in stochastic simulation such species must be converted to number of molecules which are directly proportional to the cell volume. In an effort to understand the influence of the new equation a stability analysis was performed. This elucidates how the growth factor impacts the stability of the model's limit cycles. In conclusion, a more precise model, in comparison to the base model, was created for the cell cycle as it now takes into consideration the cell volume variation

  17. Self-Organized Biological Dynamics and Nonlinear Control

    Science.gov (United States)

    Walleczek, Jan

    2006-04-01

    The frontiers and challenges of biodynamics research Jan Walleczek; Part I. Nonlinear Dynamics in Biology and Response to Stimuli: 1. External signals and internal oscillation dynamics - principal aspects and response of stimulated rhythmic processes Friedemann Kaiser; 2. Nonlinear dynamics in biochemical and biophysical systems: from enzyme kinetics to epilepsy Raima Larter, Robert Worth and Brent Speelman; 3. Fractal mechanisms in neural control: human heartbeat and gait dynamics in health and disease Chung-Kang Peng, Jeffrey M. Hausdorff and Ary L. Goldberger; 4. Self-organising dynamics in human coordination and perception Mingzhou Ding, Yanqing Chen, J. A. Scott Kelso and Betty Tuller; 5. Signal processing in biochemical reaction networks Adam P. Arkin; Part II. Nonlinear Sensitivity of Biological Systems to Electromagnetic Stimuli: 6. Electrical signal detection and noise in systems with long-range coherence Paul C. Gailey; 7. Oscillatory signals in migrating neutrophils: effects of time-varying chemical and electrical fields Howard R. Petty; 8. Enzyme kinetics and nonlinear biochemical amplification in response to static and oscillating magnetic fields Jan Walleczek and Clemens F. Eichwald; 9. Magnetic field sensitivity in the hippocampus Stefan Engström, Suzanne Bawin and W. Ross Adey; Part III. Stochastic Noise-Induced Dynamics and Transport in Biological Systems: 10. Stochastic resonance: looking forward Frank Moss; 11. Stochastic resonance and small-amplitude signal transduction in voltage-gated ion channels Sergey M. Bezrukov and Igor Vodyanoy; 12. Ratchets, rectifiers and demons: the constructive role of noise in free energy and signal transduction R. Dean Astumian; 13. Cellular transduction of periodic and stochastic energy signals by electroconformational coupling Tian Y. Tsong; Part IV. Nonlinear Control of Biological and Other Excitable Systems: 14. Controlling chaos in dynamical systems Kenneth Showalter; 15. Electromagnetic fields and biological

  18. Model-based dynamic control and optimization of gas networks

    Energy Technology Data Exchange (ETDEWEB)

    Hofsten, Kai

    2001-07-01

    This work contributes to the research on control, optimization and simulation of gas transmission systems to support the dispatch personnel at gas control centres for the decision makings in the daily operation of the natural gas transportation systems. Different control and optimization strategies have been studied. The focus is on the operation of long distance natural gas transportation systems. Stationary optimization in conjunction with linear model predictive control using state space models is proposed for supply security, the control of quality parameters and minimization of transportation costs for networks offering transportation services. The result from the stationary optimization together with a reformulation of a simplified fluid flow model formulates a linear dynamic optimization model. This model is used in a finite time control and state constrained linear model predictive controller. The deviation from the control and the state reference determined from the stationary optimization is penalized quadratically. Because of the time varying status of infrastructure, the control space is also generally time varying. When the average load is expected to change considerably, a new stationary optimization is performed, giving a new state and control reference together with a new dynamic model that is used for both optimization and state estimation. Another proposed control strategy is a control and output constrained nonlinear model predictive controller for the operation of gas transmission systems. Here, the objective is also the security of the supply, quality control and minimization of transportation costs. An output vector is defined, which together with a control vector are both penalized quadratically from their respective references in the objective function. The nonlinear model predictive controller can be combined with a stationary optimization. At each sampling instant, a non convex nonlinear programming problem is solved giving a local minimum

  19. Dynamic Colloidal Molecules Maneuvered by Light-Controlled Janus Micromotors.

    Science.gov (United States)

    Gao, Yirong; Mou, Fangzhi; Feng, Yizheng; Che, Shengping; Li, Wei; Xu, Leilei; Guan, Jianguo

    2017-07-12

    In this work, we propose and demonstrate a dynamic colloidal molecule that is capable of moving autonomously and performing swift, reversible, and in-place assembly dissociation in a high accuracy by manipulating a TiO2/Pt Janus micromotor with light irradiation. Due to the efficient motion of the TiO2/Pt Janus motor and the light-switchable electrostatic interactions between the micromotor and colloidal particles, the colloidal particles can be captured and assembled one by one on the fly, subsequently forming into swimming colloidal molecules by mimicking space-filling models of simple molecules with central atoms. The as-demonstrated dynamic colloidal molecules have a configuration accurately controlled and stabilized by regulating the time-dependent intensity of UV light, which controls the stop-and-go motion of the colloidal molecules. The dynamic colloidal molecules are dissociated when the light irradiation is turned off due to the disappearance of light-switchable electrostatic interaction between the motor and the colloidal particles. The strategy for the assembly of dynamic colloidal molecules is applicable to various charged colloidal particles. The simulated optical properties of a dynamic colloidal molecule imply that the results here may provide a novel approach for in-place building functional microdevices, such as microlens arrays, in a swift and reversible manner.

  20. Optimum control criteria for an electric driver for dynamic systems

    Directory of Open Access Journals (Sweden)

    Cecilia E. Sandoval Ruiz

    2010-04-01

    Full Text Available This work was aimed at optimising control parameters for variable speed control and post-tensioning torque by con-trolling the current of DC motors forming part of a dynamic system (in a paper-making production line by designing controllers based on absolute value and optimal symmetry criteria. A mathematical model representing an electric drive system for controlling DC motor speed and torque in a paper-rolling system was defined, a control algorithm adjusting the parameters was designed and an electric drive system simulated. The methodology consisted of a lite-rature search and a field study for defining the model and establishing the parameters to be simulated, thereby pre-senting an analytical and descriptive project. The results led to observing the efficiency of the controllers so designed and the overall drive system for which speed and current (power continue being the reference signal, leading to the conclusion that the criteria selected for control design were suitable and representing an input in developing applica-tions for monitoring dynamic electric drive systems.

  1. Dynamic Modelling and Adaptive Traction Control for Mobile Robots

    Directory of Open Access Journals (Sweden)

    A. Albagul

    2008-11-01

    Full Text Available Mobile robots have received a great deal of research in recent years. A significant amount of research has been published in many aspects related to mobile robots. Most of the research is devoted to design and develop some control techniques for robot motion and path planning. A large number of researchers have used kinematic models to develop motion control strategy for mobile robots. Their argument and assumption that these models are valid if the robot has low speed, low acceleration and light load. However, dynamic modelling of mobile robots is very important as they are designed to travel at higher speed and perform heavy duty work. This paper presents and discusses a new approach to develop a dynamic model and control strategy for wheeled mobile robot which I modelled as a rigid body that roles on two wheels and a castor. The motion control strategy consists of two levels. The first level is dealing with the dynamic of the system and denoted as `Low' level controller. The second level is developed to take care of path planning and trajectory generation.

  2. Dynamic Modelling and Adaptive Traction Control for Mobile Robots

    Directory of Open Access Journals (Sweden)

    A. Albagul

    2004-09-01

    Full Text Available Mobile robots have received a great deal of research in recent years. A significant amount of research has been published in many aspects related to mobile robots. Most of the research is devoted to design and develop some control techniques for robot motion and path planning. A large number of researchers have used kinematic models to develop motion control strategy for mobile robots. Their argument and assumption that these models are valid if the robot has low speed, low acceleration and light load. However, dynamic modelling of mobile robots is very important as they are designed to travel at higher speed and perform heavy duty work. This paper presents and discusses a new approach to develop a dynamic model and control strategy for wheeled mobile robot which I modelled as a rigid body that roles on two wheels and a castor. The motion control strategy consists of two levels. The first level is dealing with the dynamic of the system and denoted as ‘Low’ level controller. The second level is developed to take care of path planning and trajectory generation.

  3. Hybrid control and motion planning of dynamical legged locomotion

    CERN Document Server

    2012-01-01

    "This book provides a comprehensive presentation of issues and challenges faced by researchers and practicing engineers in motion planning and hybrid control of dynamical legged locomotion. The major features range from offline and online motion planning algorithms to generate desired feasible periodic walking and running motions and tow-level control schemes, including within-stride feedback laws, continuous time update laws and event-based update laws, to asymptotically stabilize the generated desired periodic orbits. This book describes the current state of the art and future directions across all domains of dynamical legged locomotion so that readers can extend proposed motion planning algorithms and control methodologies to other types of planar and 3D legged robots".

  4. Unified power flow controller: modeling and dynamic characteristic

    Science.gov (United States)

    Bach, D. H.; Loc, H. D.

    2005-12-01

    Unified power flow controller (UPFC) consists two converters. There are three purposes of this paper, firstly to illustrate the UPFC device based VSC designs, then to describe a decoupling method the UPFC's controller into two separate control systems of the shunt and the series converters respectively in realizing an appropriate coordination between them. Finally, using the Matlab tool to build a discrete simulator for the UPFC with 12 pulse converters. The simulation results show that the developed UPFC model is reflected the static and dynamic characteristics of the UPFC. The harmonics of the output of the model were analyzed. Using the simple power system with UPFC as an example, the dynamics characteristics were studied. The fault status of the system with UPFC was analyzed too.

  5. Decoupling Control of Lateral Dynamics for 4WS Vehicle

    Institute of Scientific and Technical Information of China (English)

    刘奋; 张建武; 屈求真

    2003-01-01

    An four wheel steering (4WS)feedback contrlol system that simultanelusly achieves both body sideslip angle and yaw rate responses always desirable regardless of changes in vehicle dynamics.Quantitative feedback theory (QFT)is offered as the main tool for designing the control law.Inverted decoupling is also employed to make multivariable quantitative feedback design easier.Varilus nonlinear analyses are carried ut and show that the proposed comtrol system is a robust decoupling controller which not only makesd body sideslip angle and yaw rate of the vehicle track the desired reference input sigmals respectively,byt also satisfies the rrequirement of robustness for the control system.The results also indicate that the contral system can make it available to realize ideal lateral steering dynamics tracking for vehicles.

  6. Spatial stochastic dynamics enable robust cell polarization.

    Directory of Open Access Journals (Sweden)

    Michael J Lawson

    Full Text Available Although cell polarity is an essential feature of living cells, it is far from being well-understood. Using a combination of computational modeling and biological experiments we closely examine an important prototype of cell polarity: the pheromone-induced formation of the yeast polarisome. Focusing on the role of noise and spatial heterogeneity, we develop and investigate two mechanistic spatial models of polarisome formation, one deterministic and the other stochastic, and compare the contrasting predictions of these two models against experimental phenotypes of wild-type and mutant cells. We find that the stochastic model can more robustly reproduce two fundamental characteristics observed in wild-type cells: a highly polarized phenotype via a mechanism that we refer to as spatial stochastic amplification, and the ability of the polarisome to track a moving pheromone input. Moreover, we find that only the stochastic model can simultaneously reproduce these characteristics of the wild-type phenotype and the multi-polarisome phenotype of a deletion mutant of the scaffolding protein Spa2. Significantly, our analysis also demonstrates that higher levels of stochastic noise results in increased robustness of polarization to parameter variation. Furthermore, our work suggests a novel role for a polarisome protein in the stabilization of actin cables. These findings elucidate the intricate role of spatial stochastic effects in cell polarity, giving support to a cellular model where noise and spatial heterogeneity combine to achieve robust biological function.

  7. Collective Calcium Dynamics in Networks of Communicating Cells

    Science.gov (United States)

    Byrd, Tommy; Potter, Garrett; Sun, Bo; Mugler, Andrew

    Cells can sense and encode information about their environment with remarkable precision. These properties have been studied extensively for single cells, but intercellular communication is known to be important for both single- and multicellular organisms. Here, we examine calcium dynamics of fibroblast cells exposed to external ATP stimuli, and the effects of communication and stimulus strength on cells' response. Experimental results show that increasing communication strength induces a greater fraction of cells to exhibit oscillatory calcium dynamics, but the frequencies of oscillation do not systematically shift with ATP strength. We developed a model of calcium signaling by adding noise, communication, and cell-to-cell variability to the model of Tang and Othmer. This model reproduces cells' increased tendency to oscillate as a function of communication strength, and frequency encoding is nearly removed at the global level. Our model therefore suggests that the propensity of cells to oscillate, rather than frequency encoding, determines the response to external ATP. These results suggest that the system lies near a critical boundary separating non-oscillatory and oscillatory calcium dynamics.

  8. Dynamical Allocation of Cellular Resources as an Optimal Control Problem: Novel Insights into Microbial Growth Strategies

    Science.gov (United States)

    Giordano, Nils; Mairet, Francis; Gouzé, Jean-Luc

    2016-01-01

    Microbial physiology exhibits growth laws that relate the macromolecular composition of the cell to the growth rate. Recent work has shown that these empirical regularities can be derived from coarse-grained models of resource allocation. While these studies focus on steady-state growth, such conditions are rarely found in natural habitats, where microorganisms are continually challenged by environmental fluctuations. The aim of this paper is to extend the study of microbial growth strategies to dynamical environments, using a self-replicator model. We formulate dynamical growth maximization as an optimal control problem that can be solved using Pontryagin’s Maximum Principle. We compare this theoretical gold standard with different possible implementations of growth control in bacterial cells. We find that simple control strategies enabling growth-rate maximization at steady state are suboptimal for transitions from one growth regime to another, for example when shifting bacterial cells to a medium supporting a higher growth rate. A near-optimal control strategy in dynamical conditions is shown to require information on several, rather than a single physiological variable. Interestingly, this strategy has structural analogies with the regulation of ribosomal protein synthesis by ppGpp in the enterobacterium Escherichia coli. It involves sensing a mismatch between precursor and ribosome concentrations, as well as the adjustment of ribosome synthesis in a switch-like manner. Our results show how the capability of regulatory systems to integrate information about several physiological variables is critical for optimizing growth in a changing environment. PMID:26958858

  9. Dynamical Allocation of Cellular Resources as an Optimal Control Problem: Novel Insights into Microbial Growth Strategies.

    Directory of Open Access Journals (Sweden)

    Nils Giordano

    2016-03-01

    Full Text Available Microbial physiology exhibits growth laws that relate the macromolecular composition of the cell to the growth rate. Recent work has shown that these empirical regularities can be derived from coarse-grained models of resource allocation. While these studies focus on steady-state growth, such conditions are rarely found in natural habitats, where microorganisms are continually challenged by environmental fluctuations. The aim of this paper is to extend the study of microbial growth strategies to dynamical environments, using a self-replicator model. We formulate dynamical growth maximization as an optimal control problem that can be solved using Pontryagin's Maximum Principle. We compare this theoretical gold standard with different possible implementations of growth control in bacterial cells. We find that simple control strategies enabling growth-rate maximization at steady state are suboptimal for transitions from one growth regime to another, for example when shifting bacterial cells to a medium supporting a higher growth rate. A near-optimal control strategy in dynamical conditions is shown to require information on several, rather than a single physiological variable. Interestingly, this strategy has structural analogies with the regulation of ribosomal protein synthesis by ppGpp in the enterobacterium Escherichia coli. It involves sensing a mismatch between precursor and ribosome concentrations, as well as the adjustment of ribosome synthesis in a switch-like manner. Our results show how the capability of regulatory systems to integrate information about several physiological variables is critical for optimizing growth in a changing environment.

  10. Solving Optimal Control Problems by Exploiting Inherent Dynamical Systems Structures

    Science.gov (United States)

    Flaßkamp, Kathrin; Ober-Blöbaum, Sina; Kobilarov, Marin

    2012-08-01

    Computing globally efficient solutions is a major challenge in optimal control of nonlinear dynamical systems. This work proposes a method combining local optimization and motion planning techniques based on exploiting inherent dynamical systems structures, such as symmetries and invariant manifolds. Prior to the optimal control, the dynamical system is analyzed for structural properties that can be used to compute pieces of trajectories that are stored in a motion planning library. In the context of mechanical systems, these motion planning candidates, termed primitives, are given by relative equilibria induced by symmetries and motions on stable or unstable manifolds of e.g. fixed points in the natural dynamics. The existence of controlled relative equilibria is studied through Lagrangian mechanics and symmetry reduction techniques. The proposed framework can be used to solve boundary value problems by performing a search in the space of sequences of motion primitives connected using optimized maneuvers. The optimal sequence can be used as an admissible initial guess for a post-optimization. The approach is illustrated by two numerical examples, the single and the double spherical pendula, which demonstrates its benefit compared to standard local optimization techniques.

  11. Dynamic Feedback Controlling Chaos in Current-Mode Boost Converter

    Institute of Scientific and Technical Information of China (English)

    LU Wei-Guo; ZHOU Luo-Wei; LUO Quan-Ming

    2007-01-01

    A method for the control of chaos in the current-mode boost converter is presented by using the first-order dynamic feedback control. The feedback part consists of a resistance and a capacitance in series. The system to be controlled is treated as a third-order model, and then the discrete mapping model is obtained by using the data-sampling method. By analysing the position of the maximum norm eigenvalue, the stable range of feedback gain is ascertained out and its optimization is also carried out. Finally, the results of simulation and experiment confirm the correctness of the theoretical analysis and the validity of the proposed means.

  12. Dynamic control of crystallinity in polymer film casting process

    Directory of Open Access Journals (Sweden)

    Thananchai Leephakpreeda

    2005-05-01

    Full Text Available This paper presents an approach for dynamic control of crystallinity in polymer film casting process. As known, the transients of crystallization dictate the microstructures of semi-crystalline polymer during solidification. In turn, the properties of finished products can be determined by adjustable variables in polymer film casting process such as temperature of chill roll. In this work, an experimental model of the solidification in film casting process is derived by a system identification technique. This model is used to design a digital feedback controller including a state estimator. The simulation results show the effectiveness of the proposed control technique on an extruded film.

  13. Advanced WEC Dynamics & Controls FY16 Testing Report

    Energy Technology Data Exchange (ETDEWEB)

    Coe, Ryan Geoffrey [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Bacelli, Giorgio [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Wilson, David G. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Patterson, David Charles [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2016-10-01

    A model-scale wave tank test was conducted in the interest of improving control systems design of wave energy converters (WECs). The success of most control strategies is based directly upon the availability of a reduced-order model with the ability to capture the dynamics of the system with sufficient accuracy. For this reason, the test described in this report, which is the first in a series of planned tests on WEC controls, focused on system identification (system ID) and model validation.

  14. Dynamic Performance of Grid Converters using Adaptive DC Voltage Control

    DEFF Research Database (Denmark)

    Trintis, Ionut; Sun, Bo; Guerrero, Josep M.;

    2014-01-01

    This paper investigates a controller that ensures minimum operating dc-link voltage of a back-to-back converter system. The dc-link voltage adapts its reference based on the system state, reference given by an outer loop to the dc-link voltage controller. The operating dc-link voltage should...... be kept as low as possible to increase the power conversion efficiency and increase the reliability of converters. The dynamic performance of the proposed controller is investigated by simulations and experiments....

  15. Control dynamics of severe acute respiratory syndrome transmission

    Institute of Scientific and Technical Information of China (English)

    WANG Haiying; RONG Feng; KE Fujiu; BAI Yilong

    2003-01-01

    Severe acute respiratory syndrome (SARS) is a serious disease with many puzzling features. We present a simple, dynamic model to assess the epidemic potential of SARS and the effectiveness of control measures. With this model, we analysed the SARS epidemic data in Beijing. The data fitting gives the basic case reproduction number of 2.16 leading to the outbreak, and the variation of the effective reproduction number reflecting the control effect. Noticeably, our study shows that the response time and the strength of control measures have significant effects on the scale of the outbreak and the lasting time of the epidemic.

  16. Decentralized control and filtering in interconnected dynamical systems

    CERN Document Server

    Mahmoud, Magdi S

    2011-01-01

    Based on the many approaches available for dealing with large-scale systems (LSS), Decentralized Control and Filtering in Interconnected Dynamical Systems supplies a rigorous framework for studying the analysis, stability, and control problems of LSS. Providing an overall assessment of LSS theories, it addresses model order reduction, parametric uncertainties, time delays, and control estimator gain perturbations. Taking readers on a guided tour through LSS, the book examines recent trends and approaches and reviews past methods and results from a contemporary perspective. It traces the progre

  17. Quality by control: Towards model predictive control of mammalian cell culture bioprocesses.

    Science.gov (United States)

    Sommeregger, Wolfgang; Sissolak, Bernhard; Kandra, Kulwant; von Stosch, Moritz; Mayer, Martin; Striedner, Gerald

    2017-07-01

    The industrial production of complex biopharmaceuticals using recombinant mammalian cell lines is still mainly built on a quality by testing approach, which is represented by fixed process conditions and extensive testing of the end-product. In 2004 the FDA launched the process analytical technology initiative, aiming to guide the industry towards advanced process monitoring and better understanding of how critical process parameters affect the critical quality attributes. Implementation of process analytical technology into the bio-production process enables moving from the quality by testing to a more flexible quality by design approach. The application of advanced sensor systems in combination with mathematical modelling techniques offers enhanced process understanding, allows on-line prediction of critical quality attributes and subsequently real-time product quality control. In this review opportunities and unsolved issues on the road to a successful quality by design and dynamic control implementation are discussed. A major focus is directed on the preconditions for the application of model predictive control for mammalian cell culture bioprocesses. Design of experiments providing information about the process dynamics upon parameter change, dynamic process models, on-line process state predictions and powerful software environments seem to be a prerequisite for quality by control realization. © 2017 The Authors. Biotechnology Journal published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Dynamic Microenvironment Induces Phenotypic Plasticity of Esophageal Cancer Cells Under Flow

    Science.gov (United States)

    Calibasi Kocal, Gizem; Güven, Sinan; Foygel, Kira; Goldman, Aaron; Chen, Pu; Sengupta, Shiladitya; Paulmurugan, Ramasamy; Baskin, Yasemin; Demirci, Utkan

    2016-12-01

    Cancer microenvironment is a remarkably heterogeneous composition of cellular and non-cellular components, regulated by both external and intrinsic physical and chemical stimuli. Physical alterations driven by increased proliferation of neoplastic cells and angiogenesis in the cancer microenvironment result in the exposure of the cancer cells to elevated levels of flow-based shear stress. We developed a dynamic microfluidic cell culture platform utilizing eshopagael cancer cells as model cells to investigate the phenotypic changes of cancer cells upon exposure to fluid shear stress. We report the epithelial to hybrid epithelial/mesenchymal transition as a result of decreasing E-Cadherin and increasing N-Cadherin and vimentin expressions, higher clonogenicity and ALDH positive expression of cancer cells cultured in a dynamic microfluidic chip under laminar flow compared to the static culture condition. We also sought regulation of chemotherapeutics in cancer microenvironment towards phenotypic control of cancer cells. Such in vitro microfluidic system could potentially be used to monitor how the interstitial fluid dynamics affect cancer microenvironment and plasticity on a simple, highly controllable and inexpensive bioengineered platform.

  19. Entrainment of cell division in phytoplankton with dynamic energy budgets

    Science.gov (United States)

    Muller, Erik B.; Ananthasubramaniam, Bharath; Klanjšček, Tin; Nisbet, Roger M.

    2011-11-01

    We explore the entrainment behavior of cell division in phytoplankton in the context of Dynamic Energy Budget (DEB) theory. In particular, we explore the range of DEB and environmental parameter values within which a cell divides at regular intervals in a periodic light environment with abundant nutrients and investigate the impact of parameter values on the phase of cell division. We consider three types of cells that differ in the evolution of surface area to volume ratio during the cell cycle: cells with a constant shape (isomorphs), cells with a constant surface area (V0-morphs) and cells with a constant surface area to volume ratio (V1-morphs), the latter being the default choice in studies on the population dynamics of unicellular organisms because of its desirable mathematical implications. Only in isomorphs and V0-morphs, however, cell division can be entrained to a periodic light. Regular cell division in V1 is purely coincidental, as it depends on exact choices for parameter values. We attribute this to the fact that V1-morphs lack the negative feedback of size on the dynamics of reserves in V0-morphs and isomorphs. Because entrained isomorphs and V0-morphs divide during the dark hours in our simulations, these two shapes can represent the division behavior of phytoplankton species that complete the cell cycle during the night, such as dinoflagellates and coccolithophores. A description of the division behavior of species completing the cell cycle during the day, such as silicon dependent diatoms and cyanobacteria, requires a more complex model than used in this paper. Furthermore, we explore the robustness of our findings by randomizing model parameters and introducing unevenness in biomass separation between daughter cells during cell division. We conclude that especially the entrainment in V0-morphs is relatively insensitive to perturbations.

  20. Dynamic three-dimensional micropatterned cell co-cultures within photocurable and chemically degradable hydrogels.

    Science.gov (United States)

    Sugiura, Shinji; Cha, Jae Min; Yanagawa, Fumiki; Zorlutuna, Pinar; Bae, Hojae; Khademhosseini, Ali

    2016-08-01

    In this paper we report on the development of dynamically controlled three-dimensional (3D) micropatterned cellular co-cultures within photocurable and chemically degradable hydrogels. Specifically, we generated dynamic co-cultures of micropatterned murine embryonic stem (mES) cells with human hepatocellular carcinoma (HepG2) cells within 3D hydrogels. HepG2 cells were used due to their ability to direct the differentiation of mES cells through secreted paracrine factors. To generate dynamic co-cultures, mES cells were first encapsulated within micropatterned photocurable poly(ethylene glycol) (PEG) hydrogels. These micropatterned cell-laden PEG hydrogels were subsequently surrounded by calcium alginate (Ca-Alg) hydrogels containing HepG2 cells. After 4 days, the co-culture step was halted by exposing the system to sodium citrate solution, which removed the alginate gels and the encapsulated HepG2 cells. The encapsulated mES cells were then maintained in the resulting cultures for 16 days and cardiac differentiation was analysed. We observed that the mES cells that were exposed to HepG2 cells in the co-cultures generated cells with higher expression of cardiac genes and proteins, as well as increased spontaneous beating. Due to its ability to control the 3D microenvironment of cells in a spatially and temporally regulated manner, the method presented in this study is useful for a range of cell-culture applications related to tissue engineering and regenerative medicine. Copyright © 2013 John Wiley & Sons, Ltd.