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Sample records for dynal reli sso

  1. SSoNΔ and SsoNΔlong: two thermostable esterases from the same ORF in the archaeon Sulfolobus solfataricus?

    Directory of Open Access Journals (Sweden)

    Luigi Mandrich

    2006-01-01

    Full Text Available Previously, we reported from the Sulfolobus solfataricus open reading frame (ORF SSO2517 the cloning, overexpression and characterization of an esterase belonging to the hormone-sensitive lipase (HSL family and apparently having a deletion at the N-terminus, which we named SsoNΔ. Searching the recently reported Sulfolobus acidocaldarius genome by sequence alignment, using SSO2517 as a query, allowed identity of a putative esterase (ORF SAC1105 sharing high sequence similarity (82% with SSO2517. This esterase displays an N-terminus and total length similar to other known esterases of the HSL family. Analysis of the upstream DNA sequence of SS02517 revealed the possibility of expressing a longer version of the protein with an extended N-terminus; however, no clear translation signal consistent with a longer protein version was detected. This new version of SSO2517 was cloned, over-expressed, purified and characterized. The resulting protein, named SsoNΔlong, was 15-fold more active with the substrate p-nitrophenyl hexanoate than SsoNΔ. Furthermore, SsoNΔlong and SsoNΔ displayed different substrate specificities for triacylglycerols. These results and the phylogenetic relationship between S. solfataricus and S. acidocaldarius suggest a common origin of SSO2517 and SAC1105 from an ancestral gene, followed by divergent evolution. Alternatively, a yet-to-be discovered mechanism of translation that directs the expression of SsoNΔlong under specific metabolic conditions could be hypothesized.

  2. Theoretical characterisation of the SSO, cyclic SOS and SOS isomers

    Science.gov (United States)

    Denis, Pablo A.

    2010-01-01

    The SSO, cyclic SOS and open SOS isomers have been investigated, employing the CCSD(T) methodology and the cc-pV(X + d)Z X = 3, 4, 5, 6 basis sets. The anharmonic force fields have been calculated to predict the fundamental vibrational frequencies, rotational constants, vibration-rotation corrections, anharmonic corrections to zero-point energies, and structural parameters. At the CCSD(T)/CBS limit and including corrections for scalar relativistic effects, complete quadruple excitations, spin-orbit and core-valence correlation effects, the estimated enthalpies of formation are -13.9 ± 1, 32.1 ± 1, and -51.2 ± 1 kcal/mol, for SSO, cyclic-SOS and open-SOS, respectively. Finally, it was found that the asymmetric SO stretch of the open and cyclic SOS isomers are located in a nearly identical position and it is likely that SOS also maybe present in the surface of Io.

  3. QR-SSO - Towards a QR-Code based Single Sign-On system

    OpenAIRE

    Mukhopadhyay, Syamantak; Argles, David

    2011-01-01

    Today internet users use a single identity to access multiple services. With single sign-on (SSO), users don’t have to remember separate username/password for each service provider, which helps the user to browse through the web seamlessly. SSO is however susceptible to phishing attacks. This paper describes a new anti phishing SSO model based on mobile QR code. Apart from preventing phishing attacks this new model is also safe against man in the middle & reply attacks.

  4. The Sso7d protein of Sulfolobus solfataricus: in vitro relationship among different activities

    Science.gov (United States)

    Guagliardi, Annamaria; Cerchia, Laura; Rossi, Mosè

    2002-01-01

    The physiological role of the nonspecific DNA-binding protein Sso7d from the crenarchaeon Sulfolobus solfataricus is unknown. In vitro studies have shown that Sso7d promotes annealing of complementary DNA strands (Guagliardi et al. 1997), induces negative supercoiling (Lopez-Garcia et al. 1998), and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner (Guagliardi et al. 2000). In this study, we examined the relationships among the binding of Sso7d to double-stranded DNA, its interaction with protein aggregates, and its ATPase activity. Experiments with 1-anilinonaphthalene-8-sulfonic acid as probe demonstrated that exposed hydrophobic surfaces in Sso7d are responsible for interactions with protein aggregates and double-stranded DNA, whereas the site of ATPase activity has a non-hydrophobic character. The interactions of Sso7d with double-stranded DNA and with protein aggregates are mutually exclusive events, suggesting that the disassembly activity and the DNA-related activities of Sso7d may be competitive in vivo. In contrast, the hydrolysis of ATP by Sso7d is independent of the binding of Sso7d to double-stranded DNA or protein aggregates. PMID:15803646

  5. The Sulfolobus solfataricus AAA protein sso090, a homologue of the eukaryotic ESCRT vps4 ATPase

    NARCIS (Netherlands)

    Hobel, Cedric F. V.; Albers, Sonja V.; Driessen, Arnold J. M.; Lupas, Andrei N.

    Sso0909 is a protein of the thermo-acidophilic crenarchaeon sulfolobus solfatoricus, annotated as a p60 katanin-like ATPase. We present here results supporting the hypothesis that Sso0909 is an orthologue of the eukaryotic ESCRT (enclosomal sorting complex required for transport) ATPase Vps4

  6. Rely-Guarantee Protocols

    Science.gov (United States)

    2014-05-01

    Universidade Nova de Lisboa, Caparica, Portugal. This document is a companion technical report of the paper, “Rely-Guarantee Protocols”, to appear in the...Yemini. Typestate: A programming language concept for enhancing software reliability. IEEE Trans. Software Eng., 1986. [29] K. Svendsen, L. Birkedal, and

  7. Design of pH sensitive binding proteins from the hyperthermophilic Sso7d scaffold.

    Directory of Open Access Journals (Sweden)

    Nimish Gera

    Full Text Available We have engineered pH sensitive binding proteins for the Fc portion of human immunoglobulin G (hIgG (hFc using two different strategies - histidine scanning and random mutagenesis. We obtained an hFc-binding protein, Sso7d-hFc, through mutagenesis of the Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus; Sso7d-hFc was isolated from a combinatorial library of Sso7d mutants using yeast surface display. Subsequently, we identified a pH sensitive mutant, Sso7d-his-hFc, through systematic evaluation of Sso7d-hFc mutants containing single histidine substitutions. In parallel, we also developed a yeast display screening strategy to isolate a different pH sensitive hFc binder, Sso7d-ev-hFc, from a library of mutants obtained by random mutagenesis of a pool of hFc binders. In contrast to Sso7d-hFc, both Sso7d-his-hFc and Sso7d-ev-hFc have a higher binding affinity for hFc at pH 7.4 than at pH 4.5. The Sso7d-mutant hFc binders can be recombinantly expressed at high yield in E. coli and are monomeric in solution. They bind an epitope in the CH3 domain of hFc that has high sequence homology in all four hIgG isotypes (hIgG(1-4, and recognize hIgG(1-4 as well as deglycosylated hIgG in western blotting assays. pH sensitive hFc binders are attractive candidates for use in chromatography, to achieve elution of IgG under milder pH conditions. However, the surface density of immobilized hFc binders, as well as the avidity effect arising from the multivalent interaction of dimeric hFc with the capture surface, influences the pH dependence of dissociation from the capture surface. Therefore, further studies are needed to evaluate if the Sso7d mutants identified in this study are indeed useful as affinity ligands in chromatography.

  8. The Sso7d DNA-binding protein from Sulfolobus solfataricus has ribonuclease activity.

    Science.gov (United States)

    Shehi, E; Serina, S; Fumagalli, G; Vanoni, M; Consonni, R; Zetta, L; Dehò, G; Tortora, P; Fusi, P

    2001-05-25

    Sso7d is a small, basic, abundant protein from the thermoacidophilic archaeon Sulfolobus solfataricus. Previous research has shown that Sso7d can bind double-stranded DNA without sequence specificity by placing its triple-stranded beta-sheet across the minor groove. We previously found RNase activity both in preparations of Sso7d purified from its natural source and in recombinant, purified protein expressed in Escherichia coli. This paper provides conclusive evidence that supports the assignment of RNase activity to Sso7d, shown by the total absence of activity in the single-point mutants E35L and K12L, despite the preservation of their overall structure under the assay conditions. In keeping with our observation that the residues putatively involved in RNase activity and those playing a role in DNA binding are located on different surfaces of the molecule, the activity was not impaired in the presence of DNA. If a small synthetic RNA was used as a substrate, Sso7d attacked both predicted double- and single-stranded RNA stretches, with no evident preference for specific sequences or individual bases. Apparently, the more readily attacked bonds were those intrinsically more unstable.

  9. The Polybasic Juxtamembrane Region of Sso1p Is Required for SNARE Function In Vivo†

    OpenAIRE

    Van Komen, Jeffrey S.; Bai, Xiaoyang; Rodkey, Travis L.; Schaub, Johanna; MCNEW, JAMES A.

    2005-01-01

    Exocytosis in Saccharomyces cerevisiae requires the specific interaction between the plasma membrane t-SNARE complex (Sso1/2p;Sec9p)and a vesicular v-SNARE (Snc1/2p). While SNARE proteins drive membrane fusion, many aspects of SNARE assembly and regulation are ill defined. Plasma membrane syntaxin homologs (including Sso1p) contain a highly charged juxtamembrane region between the transmembrane helix and the “SNARE domain” or core complex domain. We examined this region in vitro and in vivo b...

  10. Peningkatan Sistem Keamanan Autentikasi Single Sign On (SSO Menggunakan Algoritma AES dan One-Time Password Studi Kasus: SSO Universitas Ubudiyah Indonesia

    Directory of Open Access Journals (Sweden)

    Zuhar Musliyana

    2016-03-01

    Full Text Available Single Sign On (SSO merupakan model autentikasi independen yang diimplementasikan Universitas Ubudiyah Indonesia (UUI menggunakan Message-Digest Algorithm 5 (MD5 dan web service NuSOAP berbasis bahasa pemograman PHP. Sistem ini berjalan pada protokol Hypertext Transfer Protocol (HTTP. Faktanya penggunaan protokol HTTP ini sangat rentan terhadap berbagai jenis serangan karena data dikirim dalam bentuk plaintext tanpa ada proses enkripsi dan penerapan algoritma MD5 pada autentikasi login juga rentan terhadap serangan dictionary attacks dan rainbow tables. Disisi lain, Penggunaan web service NuSOAP juga menciptakan celah keamanan karena pengiriman dan penerimaan payload tidak dienkripsi. Saat ini diketahui sudah ada beberapa metode yang dapat digunakan untuk meningkatkan pengamanan kerentanan tersebut diantaranya yaitu menggunakan Hypertext Transfer Protocol Secure (HTTPS, Secure Hypertext Transfer Protocol (SHTTP dan Completely Automated Public Turing test to tell Computers and Humans Apart (CAPTCHA. Namun beberapa hasil penelitian terkait memperlihatkan masih terdapat beberapa kelemahan dari penggunaan HTTPS, SHTTP dan CAPTCHA. Penelitian ini mengusulkan penggunaan algoritma Advanced Encryption Standard (AES dengan pembangkit kunci dinamis dan metode One-Time Password (OTP berbasis sinkronisasi waktu dengan kombinasi salt untuk meningkatkan keamanan pada autentikasi SSO UUI. Hasil pengujian menunjukkan penerapan algoritma AES dan OTP  dapat mengamankan proses autentikasi SSO  dari serangan dictionary attack dan rainbow table.

  11. Overexpression, purification, crystallization and data collection of Sulfolobus solfataricus Sso6206, a novel highly conserved protein

    Energy Technology Data Exchange (ETDEWEB)

    McEwan, Andrew R.; Liu, Huanting; Oke, Muse; Carter, Lester; Powers, Helen; Dorward, Mark; McMahon, Stephen A.; White, Malcolm F.; Naismith, James H., E-mail: naismith@st-and.ac.uk [The Centre for Biomolecular Sciences, The University, St Andrews KY16 9ST,Scotland (United Kingdom)

    2006-03-01

    The novel protein Sso6206 has been crystallized; interestingly, the protein may form large multi-subunit oligomers. Sso6206, a 10.5 kDa protein from Sulfolobus solfataricus, has been overexpressed, purified and crystallized. The protein crystallizes in space group P6{sub 1/5}22, with unit-cell parameters a = b = 157.8, c = 307.3 Å. The crystals are hexagonal bipyramids and a data set has been collected to 2.4 Å resolution. Molecular replacement cannot be attempted as no convincing model can be identified. Crystals of selenomethionine-variant protein have not yet been obtained. Interestingly, crystal packing, gel filtration and mass spectrometry all suggest the native protein forms a multi-subunit oligomer consisting of >9 subunits.

  12. The astrometric Gaia-FUN-SSO observation campaign of 99 942 Apophis

    CERN Document Server

    Thuillot, W; Ivantsov, A; Desmars, J; Assafin, M; Eggl, S; Hestroffer, D; Rocher, P; Carry, B; David, P; Abe, L; Andreev, M; Arlot, J -E; Asami, A; Ayvasian, V; Baransky, A; Belcheva, M; Bendjoya, Ph; Bikmaev, I; Burkhonov, O A; Camci, U; Carbognani, A; Colas, F; Devyatkin, A V; Ehgamberdiev, Sh A; Enikova, P; Eyer, L; Galeev, A; Gerlach, E; Godunova, V; Golubaev, A V; Gorshanov, D L; Gumerov, R; Hashimoto, N; Helvaci, M; Ibryamov, S; Inasaridze, R Ya; Khamitov, I; Kostov, A; Kozhukhov, A M; Kozyryev, Y; Krugly, Yu N; Kryuchkovskiy, V; Kulichenko, N; Maigurova, N; Manilla-Robles, A; Martyusheva, A A; Molotov, I E; Nikolov, G; Nikolov, P; Nishiyama, K; Okumura, S; Palaversa, L; Parmonov, O; Peng, Q Y; Petrova, S N; Pinigin, G I; Pomazan, A; Rivet, J -P; Sakamoto, T; Sakhibullin, N; Sergeev, O; Sergeyev, A V; Shulga, O V; Suarez, O; Sybiryakova, Y; Takahashi, N; Tarady, V; Todd, M; Urakawa, S; Uysal, O; Vaduvescu, O; Vovk, V; Zhang, X -L

    2015-01-01

    Astrometric observations performed by the Gaia Follow-Up Network for Solar System Objects (Gaia-FUN-SSO) play a key role in ensuring that moving objects first detected by ESA's Gaia mission remain recoverable after their discovery. An observation campaign on the potentially hazardous asteroid (99 942) Apophis was conducted during the asteroid's latest period of visibility, from 12/21/2012 to 5/2/2013, to test the coordination and evaluate the overall performance of the Gaia-FUN-SSO . The 2732 high quality astrometric observations acquired during the Gaia-FUN-SSO campaign were reduced with the Platform for Reduction of Astronomical Images Automatically (PRAIA), using the USNO CCD Astrograph Catalogue 4 (UCAC4) as a reference. The astrometric reduction process and the precision of the newly obtained measurements are discussed. We compare the residuals of astrometric observations that we obtained using this reduction process to data sets that were individually reduced by observers and accepted by the Minor Plane...

  13. Differential active site loop conformations mediate promiscuous activities in the lactonase SsoPox.

    Directory of Open Access Journals (Sweden)

    Julien Hiblot

    Full Text Available Enzymes are proficient catalysts that enable fast rates of Michaelis-complex formation, the chemical step and products release. These different steps may require different conformational states of the active site that have distinct binding properties. Moreover, the conformational flexibility of the active site mediates alternative, promiscuous functions. Here we focused on the lactonase SsoPox from Sulfolobus solfataricus. SsoPox is a native lactonase endowed with promiscuous phosphotriesterase activity. We identified a position in the active site loop (W263 that governs its flexibility, and thereby affects the substrate specificity of the enzyme. We isolated two different sets of substitutions at position 263 that induce two distinct conformational sampling of the active loop and characterized the structural and kinetic effects of these substitutions. These sets of mutations selectively and distinctly mediate the improvement of the promiscuous phosphotriesterase and oxo-lactonase activities of SsoPox by increasing active-site loop flexibility. These observations corroborate the idea that conformational diversity governs enzymatic promiscuity and is a key feature of protein evolvability.

  14. Strong Enrichment of Aromatic Residues in Binding Sites from a Charge-neutralized Hyperthermostable Sso7d Scaffold Library*

    Science.gov (United States)

    Kiefer, Jonathan D.; Srinivas, Raja R.; Lobner, Elisabeth; Tisdale, Alison W.; Mehta, Naveen K.; Yang, Nicole J.; Tidor, Bruce; Wittrup, K. Dane

    2016-01-01

    The Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus is an attractive binding scaffold because of its small size (7 kDa), high thermal stability (Tm of 98 °C), and absence of cysteines and glycosylation sites. However, as a DNA-binding protein, Sso7d is highly positively charged, introducing a strong specificity constraint for binding epitopes and leading to nonspecific interaction with mammalian cell membranes. In the present study, we report charge-neutralized variants of Sso7d that maintain high thermal stability. Yeast-displayed libraries that were based on this reduced charge Sso7d (rcSso7d) scaffold yielded binders with low nanomolar affinities against mouse serum albumin and several epitopes on human epidermal growth factor receptor. Importantly, starting from a charge-neutralized scaffold facilitated evolutionary adaptation of binders to differentially charged epitopes on mouse serum albumin and human epidermal growth factor receptor, respectively. Interestingly, the distribution of amino acids in the small and rigid binding surface of enriched rcSso7d-based binders is very different from that generally found in more flexible antibody complementarity-determining region loops but resembles the composition of antibody-binding energetic hot spots. Particularly striking was a strong enrichment of the aromatic residues Trp, Tyr, and Phe in rcSso7d-based binders. This suggests that the rigidity and small size of this scaffold determines the unusual amino acid composition of its binding sites, mimicking the energetic core of antibody paratopes. Despite the high frequency of aromatic residues, these rcSso7d-based binders are highly expressed, thermostable, and monomeric, suggesting that the hyperstability of the starting scaffold and the rigidness of the binding surface confer a high tolerance to mutation. PMID:27582495

  15. Phage display selection of tight specific binding variants from a hyperthermostable Sso7d scaffold protein library.

    Science.gov (United States)

    Zhao, Ning; Schmitt, Margaret A; Fisk, John D

    2016-04-01

    Antibodies, the quintessential biological recognition molecules, are not ideal for many applications because of their large size, complex modifications, and thermal and chemical instability. Identifying alternative scaffolds that may be evolved into tight, specific binding molecules with improved physical properties is of increasing interest, particularly for biomedical applications in resource-limited environments. Hyperthermophilic organisms, such as Sulfolobus solfataricus, are an attractive source of highly stable proteins that may serve as starting points for alternative molecular recognition scaffolds. We describe the first application of phage display to identify binding proteins based on the S. solfataricus protein Sso7d scaffold. Sso7d is a small cysteine-free DNA-binding protein (approximately 7 kDa, 63 amino acids), with a melting temperature of nearly 100 °C. Tight-binding Sso7d variants were selected for a diverse set of protein targets from a 10(10) member library, demonstrating the versatility of the scaffold. These Sso7d variants are able to discriminate among closely related human, bovine and rabbit serum albumins. Equilibrium dissociation constants in the nanomolar to low micromolar range were measured via competitive ELISA. Importantly, the Sso7d variants continue to bind their targets in the absence of the phage context. Furthermore, phage-displayed Sso7d variants retain their binding affinity after exposure to temperatures up to 70 °C. Taken together, our results suggest that the Sso7d scaffold will be a complementary addition to the range of non-antibody scaffold proteins that may be utilized in phage display. Variants of hyperthermostable binding proteins have potential applications in diagnostics and therapeutics for environments with extreme conditions of storage and deployment.

  16. The Gaia-FUN-SSO observation campaign of 99942 Apophis: A preliminary test for the network

    CERN Document Server

    Bancelin, D; Ivantsov, A; Desmars, J; Assafin, M; Eggl, S; Hestroffer, D; Rocher, P; Carry, B; David, P

    2016-01-01

    In order to test the coordination and evaluate the overall performance of the Gaia-FUN-SSO, an observation campaign on the Potentially Hazardous Asteroid (99 942) Apophis was conducted from 12/21/2012 to 5/2/2013 providing 2732 high quality astrometric observations. We show that a consistent reduction of astrometric campaigns with reliable stellar catalogs substantially improves the quality of astrometric results. We present evidence that the new data will help to reduce the orbit uncertainty of Apophis during its close approach in 2029.

  17. A recombinase paralog from the hyperthermophilic crenarchaeon Sulfolobus solfataricus enhances SsoRadA ssDNA binding and strand displacement.

    Science.gov (United States)

    Graham, William J; Haseltine, Cynthia A

    2013-02-15

    Homologous recombination (HR) is a major pathway for the repair of double-strand DNA breaks, a highly deleterious form of DNA damage. The main catalytic protein in HR is the essential RecA-family recombinase, which is conserved across all three domains of life. Eukaryotes and archaea encode varying numbers of proteins paralogous to their main recombinase. Although there is increasing evidence for the functions of some of these paralog proteins, overall their mechanism of action remains largely unclear. Here we present the first biochemical characterization of one of the paralog proteins, SsoRal3, from the crenarchaeaon Sulfolobus solfataricus. The SsoRal3 protein is a ssDNA-dependent ATPase that can catalyze strand invasion at both saturating and subsaturating concentrations. It can bind both ssDNA and dsDNA, but its binding preference is altered by the presence or absence of ATP. Addition of SsoRal3 to SsoRadA nucleoprotein filaments reduces total ATPase activity. Subsaturating concentrations of SsoRal3 increase the ssDNA binding activity of SsoRadA approximately 9-fold and also increase the persistence of SsoRadA catalyzed strand invasion products. Overall, these results suggest that SsoRal3 functions to stabilize the SsoRadA presynaptic filament. Copyright © 2012 Elsevier B.V. All rights reserved.

  18. Lying relies on the truth.

    Science.gov (United States)

    Debey, Evelyne; De Houwer, Jan; Verschuere, Bruno

    2014-09-01

    Cognitive models of deception focus on the conflict-inducing nature of the truth activation during lying. Here we tested the counterintuitive hypothesis that the truth can also serve a functional role in the act of lying. More specifically, we examined whether the construction of a lie can involve a two-step process, where the first step entails activating the truth, based upon which a lie response can be formulated in a second step. To investigate this hypothesis, we tried to capture the covert truth activation in a reaction-time based deception paradigm. Together with each question, we presented either the truth or lie response as distractors. If lying depends on the covert activation of the truth, deceptive responses would thus be facilitated by truth distractors relative to lie distractors. Our results indeed revealed such a "covert congruency" effect, both in errors and reaction times (Experiment 1). Moreover, stimulating participants to use the distractor information by increasing the proportion of truth distractor trials enlarged the "covert congruency" effects, and as such confirmed that the effects operate at a covert response level (Experiment 2). Our findings lend support to the idea that lying relies on a first step of truth telling, and call for a shift in theoretical thinking that highlights both the functional and interfering properties of the truth activation in the lying process. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. Interactions within the yeast t-SNARE Sso1p that control SNARE complex assembly.

    Science.gov (United States)

    Munson, M; Chen, X; Cocina, A E; Schultz, S M; Hughson, F M

    2000-10-01

    In the eukaryotic secretory and endocytic pathways, transport vesicles shuttle cargo among intracellular organelles and to and from the plasma membrane. Cargo delivery entails fusion of the transport vesicle with its target, a process thought to be mediated by membrane bridging SNARE protein complexes. Temporal and spatial control of intracellular trafficking depends in part on regulating the assembly of these complexes. In vitro, SNARE assembly is inhibited by the closed conformation adopted by the syntaxin family of SNAREs. To visualize this closed conformation directly, the X-ray crystal structure of a yeast syntaxin, Sso1p, has been determined and refined to 2.1 A resolution. Mutants designed to destabilize the closed conformation exhibit accelerated rates of SNARE assembly. Our results provide insight into the mechanism of SNARE assembly and its intramolecular and intermolecular regulation.

  20. Crystal structure of a novel archaeal AAA+ ATPase SSO1545 from Sulfolobus solfataricus

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Qingping; Rife, Christopher L.; Carlton, Dennis; Miller, Mitchell D.; Krishna, S. Sri; Elsliger, Marc-André; Abdubek, Polat; Astakhova, Tamara; Chiu, Hsiu-Ju; Clayton, Thomas; Duan, Lian; Feuerhelm, Julie; Grzechnik, Slawomir K.; Hale, Joanna; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K.; Klock, Heath E.; Knuth, Mark W.; Kumar, Abhinav; McMullan, Daniel; Morse, Andrew T.; Nigoghossian, Edward; Okach, Linda; Oommachen, Silvya; Paulsen, Jessica; Reyes, Ron; van den Bedem, Henry; Hodgson, Keith O.; Wooley, John; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A.; (Scripps); (SSR); (SSRL); (JCSG); (UCSD)

    2009-08-28

    Signal transduction ATPases with numerous domains (STAND), a large class of P-loop NTPases, belong to AAA+ ATPases. They include AP(apoptotic)-ATPases (e.g., animal apoptosis regulators CED4/Apaf-1, plant disease resistance proteins, and bacterial AfsR-like transcription regulators), NACHT NTPases (e.g. CARD4, NAIP, Het-E-1, TLP1), and several other less well-characterized families. STAND differ from other P-loop NTPases by their unique sequence motifs, which include an hhGRExE (h, hydrophobic; x, any residue) motif at the N-terminal region, a GxP/GxxP motif at the C-terminal region of the NTPase domain, in addition to a C-terminal helical domain and additional domains such as WD40, TPR, LRR or catalytic modules. Despite significant biological interests, structural coverage of STAND proteins is very limited and only two other structures are currently known: the cell death regulators Apaf-1 and CED-4. Here, we report the crystal structure of SSO1545 from Sulfolobus solfataricus, which was determined using the semi-automated, high-throughput pipeline of the Joint Center for Structural Genomics (JCSG; http://www.jcsg.org), as part of the National Institute of General Medical Sciences' Protein Structure Initiative (PSI). SSO1545 (NP-342973.1), a representative of the archaeal STANDs, is a member of Pfam PF01637 and encodes a protein of 356 residues with calculated molecular weight and isoelectric point of 41.7 kD and 8.2, respectively.

  1. Global identification of hnRNP A1 binding sites for SSO-based splicing modulation

    DEFF Research Database (Denmark)

    Bruun, Gitte H; Doktor, Thomas K; Borch-Jensen, Jonas;

    2016-01-01

    for this deregulation by blocking other SREs with splice-switching oligonucleotides (SSOs). However, the location and sequence of most SREs are not well known. RESULTS: Here, we used individual-nucleotide resolution crosslinking immunoprecipitation (iCLIP) to establish an in vivo binding map for the key splicing...... regulatory factor hnRNP A1 and to generate an hnRNP A1 consensus binding motif. We find that hnRNP A1 binding in proximal introns may be important for repressing exons. We show that inclusion of the alternative cassette exon 3 in SKA2 can be significantly increased by SSO-based treatment which blocks an iCLIP......-identified hnRNP A1 binding site immediately downstream of the 5' splice site. Because pseudoexons are well suited as models for constitutive exons which have been inactivated by pathogenic mutations in SREs, we used a pseudoexon in MTRR as a model and showed that an iCLIP-identified hnRNP A1 binding site...

  2. Research on Shaft Subsynchronous Oscillation Characteristics of Parallel Generators and SSDC Application in Mitigating SSO of Multi-Generators

    Directory of Open Access Journals (Sweden)

    Shen Wang

    2015-02-01

    Full Text Available Subsynchronous oscillation (SSO of generators caused by high voltage direct current (HVDC systems can be solved by applying supplemental subsynchronous damping controller (SSDC. SSDC application in mitigating SSO of single-generator systems has been studied intensively. This paper focuses on SSDC application in mitigating SSO of multi-generator systems. The phase relationship of the speed signals of the generators under their common mechanical natural frequencies is a key consideration in SSDC design. The paper studies in detail the phase relationship of the speed signals of two generators in parallel under their shared mechanical natural frequency, revealing regardless of whether the two generators are identical or not, there always exists a common-mode and an anti-mode under their common natural frequency, and the phase relationship of the speed signals of the generators depends on the extent to which the anti-mode is stimulated. The paper further demonstrates that to guarantee the effectiveness of SSDC, the anti-phase mode component of its input signal should be eliminated. Based on the above analysis, the paper introduces the design process of SSDC for multi-generator systems and verifies its effectiveness through simulation in Power Systems Computer Aided Design/Electromagnetic Transients including Direct Current (PSCAD/EMTDC.

  3. Brain Relies on Two Timekeepers for Sleep

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_160407.html Brain Relies on Two Timekeepers for Sleep Study shows ... internal "hourglass" affect how different parts of your brain respond to sleep deprivation, a new study shows. ...

  4. The Arginine Pairs and C-Termini of the Sso7c4 from Sulfolobus solfataricus Participate in Binding and Bending DNA

    Science.gov (United States)

    Huang, Chun-Hsiang; Ko, Tzu-Ping; Chiang, Cheng-Hung; Lin, Kuan-Fu; Chang, Yuan-Chih; Lin, Po-Yen; Tsai, Hui-Hsu Gavin; Wang, Andrew H.-J.

    2017-01-01

    The Sso7c4 from Sulfolobus solfataricus forms a dimer, which is believed to function as a chromosomal protein involved in genomic DNA compaction and gene regulation. Here, we present the crystal structure of wild-type Sso7c4 at a high resolution of 1.63 Å, showing that the two basic C-termini are disordered. Based on the fluorescence polarization (FP) binding assay, two arginine pairs, R11/R22′ and R11′/R22, on the top surface participate in binding DNA. As shown in electron microscopy (EM) images, wild-type Sso7c4 compacts DNA through bridging and bending interactions, whereas the binding of C-terminally truncated proteins rigidifies and opens DNA molecules, and no compaction of the DNA occurs. Moreover, the FP, EM and fluorescence resonance energy transfer (FRET) data indicated that the two basic and flexible C-terminal arms of the Sso7c4 dimer play a crucial role in binding and bending DNA. Sso7c4 has been classified as a repressor-like protein because of its similarity to Escherichia coli Ecrep 6.8 and Ecrep 7.3 as well as Agrobacterium tumefaciens ACCR in amino acid sequence. Based on these data, we proposed a model of the Sso7c4-DNA complex using a curved DNA molecule in the catabolite activator protein-DNA complex. The DNA end-to-end distance measured with FRET upon wild-type Sso7c4 binding is almost equal to the distance measured in the model, which supports the fidelity of the proposed model. The FRET data also confirm the EM observation showing that the binding of wild-type Sso7c4 reduces the DNA length while the C-terminal truncation does not. A functional role for Sso7c4 in the organization of chromosomal DNA and/or the regulation of gene expression through bridging and bending interactions is suggested. PMID:28068385

  5. An Atom Trap Relying on Optical Pumping

    CERN Document Server

    Bouyer, P; Dahan, M B; Michaud, A; Salomon, C; Dalibard, J

    1994-01-01

    We have investigated a new radiation pressure trap which relies on optical pumping and does not require any magnetic field. It employs six circularly polarized divergent beams and works on the red of a $J_{g} \\longrightarrow J_{e} = J_{g} + 1$ atomic transition with $J_{g} \\geq 1/2$. We have demonstrated this trap with cesium atoms from a vapour cell using the 852 nm $J_{g} = 4 \\longrightarrow J_{e} = 5$ resonance transition. The trap contained up to $3 \\cdot 10^{7}$ atoms in a cloud of $1/\\sqrt{e}$ radius of 330 $\\mu$m.

  6. Evidence that the xylanase activity from Sulfolobus solfataricus Oalpha is encoded by the endoglucanase precursor gene (sso1354) and characterization of the associated cellulase activity.

    Science.gov (United States)

    Maurelli, Luisa; Giovane, Alfonso; Esposito, Alessandra; Moracci, Marco; Fiume, Immacolata; Rossi, Mosè; Morana, Alessandra

    2008-09-01

    Sulfolobus solfataricus strain Oalpha was previously isolated for its ability to grow on minimal medium supplemented with xylan as a carbon source. The strain exhibited thermostable xylanase activity but several attempts to identify the gene encoding for the activity failed. Further studies showed that the xylanase displayed activity on carboxymethylcellulose (CMC) and the new activity was characterized. It exhibited an optimal temperature and pH of 95 degrees C and 3.5, respectively, and a half-life of 53 min at 95 degrees C. The enzyme, which was demonstrated to be glycosylated, hydrolyzed CMC in an endo-manner releasing cellobiose and other cello-oligomers. Analysis of the tryptic fragments by tandem mass spectrometry led to identification of the endoglucanase precursor, encoded by the sso1354 gene, as the protein possessing dual activity. The efficiency of the SSO1354 protein in degrading cellulosic and hemicellulosic fractions contained in agronomic residues was tested at low pH and high temperature. Cellulose and xylan were degraded to glucose and xylose at 90 degrees C, pH 4 by an enzyme mix consisting of SSO1354 and additional glycosyl hydrolases from S. solfataricus Oalpha. Given its role in saccharification processes requiring high temperatures and acidic environments, SSO1354 represents an interesting candidate for the utilization of agro-industrial waste for fuel production.

  7. Enabling Secure XMPP Communications in Federated IoT Clouds Through XEP 0027 and SAML/SASL SSO

    Science.gov (United States)

    Celesti, Antonio; Fazio, Maria; Villari, Massimo

    2017-01-01

    Nowadays, in the panorama of Internet of Things (IoT), finding a right compromise between interactivity and security is not trivial at all. Currently, most of pervasive communication technologies are designed to work locally. As a consequence, the development of large-scale Internet services and applications is not so easy for IoT Cloud providers. The main issue is that both IoT architectures and services have started as simple but they are becoming more and more complex. Consequently, the web service technology is often inappropriate. Recently, many operators in both academia and industry fields are considering the possibility to adopt the eXtensible Messaging and Presence Protocol (XMPP) for the implementation of IoT Cloud communication systems. In fact, XMPP offers many advantages in term of real-time capabilities, efficient data distribution, service discovery and inter-domain communication compared to other technologies. Nevertheless, the protocol lacks of native security, data confidentiality and trustworthy federation features. In this paper, considering an XMPP-based IoT Cloud architectural model, we discuss how can be possible to enforce message signing/encryption and Single-Sign On (SSO) authentication respectively for secure inter-module and inter-domain communications in a federated environment. Experiments prove that security mechanisms introduce an acceptable overhead, considering the obvious advantages achieved in terms of data trustiness and privacy. PMID:28178214

  8. Enabling Secure XMPP Communications in Federated IoT Clouds Through XEP 0027 and SAML/SASL SSO.

    Science.gov (United States)

    Celesti, Antonio; Fazio, Maria; Villari, Massimo

    2017-02-07

    Nowadays, in the panorama of Internet of Things (IoT), finding a right compromise between interactivity and security is not trivial at all. Currently, most of pervasive communication technologies are designed to work locally. As a consequence, the development of large-scale Internet services and applications is not so easy for IoT Cloud providers. The main issue is that both IoT architectures and services have started as simple but they are becoming more and more complex. Consequently, the web service technology is often inappropriate. Recently, many operators in both academia and industry fields are considering the possibility to adopt the eXtensible Messaging and Presence Protocol (XMPP) for the implementation of IoT Cloud communication systems. In fact, XMPP offers many advantages in term of real-time capabilities, efficient data distribution, service discovery and inter-domain communication compared to other technologies. Nevertheless, the protocol lacks of native security, data confidentiality and trustworthy federation features. In this paper, considering an XMPP-based IoT Cloud architectural model, we discuss how can be possible to enforce message signing/encryption and Single-Sign On (SSO) authentication respectively for secure inter-module and inter-domain communications in a federated environment. Experiments prove that security mechanisms introduce an acceptable overhead, considering the obvious advantages achieved in terms of data trustiness and privacy.

  9. Enabling Secure XMPP Communications in Federated IoT Clouds Through XEP 0027 and SAML/SASL SSO

    Directory of Open Access Journals (Sweden)

    Antonio Celesti

    2017-02-01

    Full Text Available Nowadays, in the panorama of Internet of Things (IoT, finding a right compromise between interactivity and security is not trivial at all. Currently, most of pervasive communication technologies are designed to work locally. As a consequence, the development of large-scale Internet services and applications is not so easy for IoT Cloud providers. The main issue is that both IoT architectures and services have started as simple but they are becoming more and more complex. Consequently, the web service technology is often inappropriate. Recently, many operators in both academia and industry fields are considering the possibility to adopt the eXtensible Messaging and Presence Protocol (XMPP for the implementation of IoT Cloud communication systems. In fact, XMPP offers many advantages in term of real-time capabilities, efficient data distribution, service discovery and inter-domain communication compared to other technologies. Nevertheless, the protocol lacks of native security, data confidentiality and trustworthy federation features. In this paper, considering an XMPP-based IoT Cloud architectural model, we discuss how can be possible to enforce message signing/encryption and Single-Sign On (SSO authentication respectively for secure inter-module and inter-domain communications in a federated environment. Experiments prove that security mechanisms introduce an acceptable overhead, considering the obvious advantages achieved in terms of data trustiness and privacy.

  10. 基于虚拟身份的单点登录方案%A Mechanism of SSO Based On Virtual Identity

    Institute of Scientific and Technical Information of China (English)

    庄小君; 吴昊

    2007-01-01

    单点登录(SSO)技术是Internet中一项非常重要的安全技术.随着Internet的广泛使用,如何实现单点登录也是一个热点问题.文章首先介绍了单点登录技术的基本原理,然后介绍了微软Passport单点登录协议以及自由联盟的单点登录协议.提出了一种基于虚拟身份的单点登录方案,并把此方案与微软Passport单点登录协议以及自由联盟的单点登录协议进行了比较.

  11. 540 nm diode-pumped passively Nd:SSO/KTP green laser with a single-walled carbon nanotube saturable absorber

    Science.gov (United States)

    Cheng, K.; Zhao, S. Z.; Yang, K. J.; Li, G. Q.; Li, D. C.; Zhang, G.; Wang, Y. G.; Zheng, L. H.; Wu, F.; Su, L. B.; Xu, J.

    2013-05-01

    The fluorescence spectrum of the Nd:Sc2SiO5 (Nd:SSO) crystal is measured. The central wavelength of the fluorescence band is 1080.1 nm with a full width at half-maximum (FWHM) of 3.4 nm. By using a single-walled carbon nanotube saturable absorber as a passive Q-switcher, a diode-pumped passively Q-switched intracavity frequency doubling Nd:Sc2SiO5/KTP green laser operating at 540 nm is demonstrated for the first time. The maximum average output power is 0.89 W at an incident pump power of 11.8 W, corresponding to an optical conversion efficiency of 7.5% and a slope efficiency of 10.2%. At the same incident pump power, a minimum pulse width of 94.8 ns and a maximum peak power of 19.1 W can be obtained.

  12. Design and implementation of SSO Construction System Based on CAS%基于CAS构建SSO系统的设计与实现

    Institute of Scientific and Technical Information of China (English)

    李勇

    2012-01-01

    The number of enterprise business system is increasing with the application of information technology in enterprise. As usual, each business system needs a separate login, which inevitably brings a lot of inconvenience both in operation and management. Designing SSO system for the enterprise is an effective solution to solve the problem. A kind of solution named SSO system is discussed based on CAS construction and through the EIP system of the Agricultural Development Bank of China using this kind of solution to demonstrates the scheme.%随着信息技术在企业应用中的普及,企业业务系统的数量不断增加.通常,进入每个业务系统都需要进行一次单独的登录,这势必会在操作和管理上带来诸多不便.因此,为企业设计SSO(Single Sign On,单点登录)系统是解决以上问题的一个有效方案,论述一种基于CAS(Central Authentication Service,中央认证服务)构建SSO系统的方案,并通过在中国农业发展银行EIP系统中使用解决过程中的实践,从而论证了该方案的可行性.

  13. Frequency of alleles and haplotypes of the human leukocyte antigen system in Bauru, São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Luana de Cassia Salvadori

    2014-04-01

    Full Text Available Background: HLA allele identification is used in bone marrow transplant programs as HLA compatibility between the donor and recipient may prevent graft rejection. Objective: This study aimed to estimate the frequency of alleles and haplotypes of the HLA system in the region of Bauru and compare these with the frequencies found in other regions of the country. Methods: HLA-A*, HLA-B*, and HLA-DRB1* allele frequencies and haplotypes were analyzed in a sample of 3542 volunteer donors at the National Registry of Voluntary Bone Marrow Donors (REDOME in Bauru. HLA low resolution typing was performed using reverse line blot with the Dynal Reli(tm SSO-HLA Typing Kit and automated Dynal AutoReli(tm48 device (Invitrogen, USA. Results: Twenty, 36, and 13 HLA-A*, HLA-B*, and HLA-DRB1* allele groups, respectively, were identified. The most common alleles for each locus were HLA-A*02, HLA-B*35, and HLA-DRB1*07. The most frequent haplotype was A*01-B*08-DRB1*03. Allele and haplotype frequencies were compared to other regions in Brazil and the similarities and differences among populations are shown. Conclusion: The knowledge of the immunogenic profile of a population contributes to the comprehension of the historical and anthropological aspects of different regions. Moreover, this helps to find suitable donors quickly, thereby shortening waiting lists for transplants and thus increasing survival rates among recipients.

  14. Relying on topic subsets for system ranking estimation

    NARCIS (Netherlands)

    Hauff, C.; Hiemstra, Djoerd; de Jong, Franciska M.G.; Azzopardi, Leif

    2009-01-01

    Ranking a number of retrieval systems according to their retrieval effectiveness without relying on costly relevance judgments was first explored by Soboroff et al [6]. Over the years, a number of alternative approaches have been proposed. We perform a comprehensive analysis of system ranking

  15. Relying on topic subsets for system ranking estimation

    NARCIS (Netherlands)

    Hauff, C.; Hiemstra, Djoerd; de Jong, Franciska M.G.; Azzopardi, Leif

    2009-01-01

    Ranking a number of retrieval systems according to their retrieval effectiveness without relying on costly relevance judgments was first explored by Soboroff et al [6]. Over the years, a number of alternative approaches have been proposed. We perform a comprehensive analysis of system ranking estima

  16. Covert face recognition relies on affective valence in congenital prosopagnosia.

    Science.gov (United States)

    Bate, Sarah; Haslam, Catherine; Jansari, Ashok; Hodgson, Timothy L

    2009-06-01

    Dominant accounts of covert recognition in prosopagnosia assume subthreshold activation of face representations created prior to onset of the disorder. Yet, such accounts cannot explain covert recognition in congenital prosopagnosia, where the impairment is present from birth. Alternatively, covert recognition may rely on affective valence, yet no study has explored this possibility. The current study addressed this issue in 3 individuals with congenital prosopagnosia, using measures of the scanpath to indicate recognition. Participants were asked to memorize 30 faces paired with descriptions of aggressive, nice, or neutral behaviours. In a later recognition test, eye movements were monitored while participants discriminated studied from novel faces. Sampling was reduced for studied--nice compared to studied--aggressive faces, and performance for studied--neutral and novel faces fell between these two conditions. This pattern of findings suggests that (a) positive emotion can facilitate processing in prosopagnosia, and (b) covert recognition may rely on emotional valence rather than familiarity.

  17. ReLIS: Research Papers on Library and Information Science

    OpenAIRE

    Subirats-Coll, Imma; Barrueco, José-Manuel

    2002-01-01

    ReLIS is realtionated with the international tendencies of open archives of scientific Literature, which guarantees its quality, continuity and also extension possibility. ] applies to the philosophy harnessed by the Open Archives Initiative, and of its future products come from that, at the moment are in phase of study on the part of our equipment. OAI develop and promote the interoperability to facilitate the diffusion of contents, to offer the access to e-prints and to increase the communi...

  18. Suitability study of sampled-data model of TCSC for SSO analysis%TCSC采样-数据模型在次同步振荡分析中的适用性研究

    Institute of Scientific and Technical Information of China (English)

    向昌明; 吴熙; 蒋平

    2013-01-01

    Since frequency of the analysis component of Subsynchronous Oscillation (SSO) problem is neither at the power frequency nor within the range of low frequency, Quasi-Steady-State (QSS) model cannot be used to analyze SSO. For the same reason, we also cannot consider the analysis component of SSO as electricity signals at power frequency with low frequency modulation to analyze SSO problem. The dynamic model TCSC must be effective throughout the whole frequency range of SSO. Hence, verification of the suitability of TCSC dynamic model is of great significance. This paper proposes a method to verify the suitability of TCSC dynamic model through comparing damping characteristics throughout the whole frequency range of SSO. On one hand, sample-data model of TCSC is adopted, and small signal analysis method is utilized to establish state space matrices of system. Then a series of matric transformation is applied so as to obtain electrical damping characteristics of system. On the other hand, time simulation model is constructed in PSCAD, where the model of TCSC is precise to the device-level. Then, test signal method is adopted to obtain electrical damping characteristics of system. Finally, electrical damping characteristics obtained by the two methods are compared. The simulation result shows that the sample-data-based model of TCSC is accurate and suitable for the analysis of SSO problem.%由于电力系统次同步振荡(SSO)问题要分析的是既非工频又非低频的成分,故不能采用工频准稳态模型或认为工频电量上有低频调制来进行分析,使用的TCSC动态数学模型必须在整个次同步振荡范围内都有效,因此TCSC动态模型适用性验证的工作非常重要。提出通过对比含TCSC系统在整个次同步频域内的阻尼特性来验证TCSC动态模型的适用性。一方面,TCSC采用采样-数据模型,运用小扰动分析法建立系统的状态空间矩阵,并通过矩阵变换直接求取系统

  19. Number Comparison and Number Line Estimation Rely on Different Mechanisms

    Directory of Open Access Journals (Sweden)

    Delphine Sasanguie

    2013-12-01

    Full Text Available The performance in comparison and number line estimation is assumed to rely on the same underlying representation, similar to a compressed mental number line that becomes more linear with age. We tested this assumption explicitly by examining the relation between the linear/logarithmic fit in a non-symbolic number line estimation task and the size effect (SE in a non-symbolic comparison task in first-, second-, and third graders. In two experiments, a correlation between the estimation pattern in number line estimation and the SE in comparison was absent. An ANOVA showed no difference between the groups of children with a linear or a logarithmic representation considering their SE in comparison. This suggests that different mechanisms underlie both basic number processing tasks.

  20. da Vinci decoded: does da Vinci stereopsis rely on disparity?

    Science.gov (United States)

    Tsirlin, Inna; Wilcox, Laurie M; Allison, Robert S

    2012-11-01

    In conventional stereopsis, the depth between two objects is computed based on the retinal disparity in the position of matching points in the two eyes. When an object is occluded by another object in the scene, so that it is visible only in one eye, its retinal disparity cannot be computed. Nakayama and Shimojo (1990) found that a precept of quantitative depth between the two objects could still be established for such stimuli and proposed that this precept is based on the constraints imposed by occlusion geometry. They named this and other occlusion-based depth phenomena "da Vinci stereopsis." Subsequent research found quantitative depth based on occlusion geometry in several other classes of stimuli grouped under the term da Vinci stereopsis. However, Nakayama and Shimojo's findings were later brought into question by Gillam, Cook, and Blackburn (2003), who suggested that quantitative depth in their stimuli was perceived based on conventional disparity. In order to understand whether da Vinci stereopsis relies on one type of mechanism or whether its function is stimulus dependent we examine the nature and source of depth in the class of stimuli used by Nakayama and Shimojo (1990). We use three different psychophysical and computational methods to show that the most likely source for depth in these stimuli is occlusion geometry. Based on these experiments and previous data we discuss the potential mechanisms responsible for processing depth from monocular features in da Vinci stereopsis.

  1. EMTP-Based Smart Optimization of TCSC Parameters Oriented for Mitigating Wind Farm SSO%面向风电场SSO抑制的TCSC参数电磁暂态智能优化方法

    Institute of Scientific and Technical Information of China (English)

    黄杰; 陈武晖; 董德勇; 谭伦农

    2015-01-01

    抑制风电场次同步振荡(sub-synchronous oscillations, SSO)的晶闸管可控串联补偿装置(thyristor controlled series compensation,TCSC)参数优化问题是强非线性参数优化问题,很难用解析方法求解.文中将单纯形算法(Simplex)与电磁暂态仿真程序结合的非线性参数优化方法引入到抑制SSO 的 TCSC 参数的优化.该算法使用电磁暂态程序的仿真结果计算优化目标函数值,Simplex算法应用电磁暂态程序计算的优化目标函数结果智能地指导 TCSC 参数寻优方向,快速可靠地寻到最优参数,缩短仿真耗时.最后,针对风电不确定性,初步分析了风速变化对参数优化结果的影响,根据所采用的单机等值系统仿真结果分析,风速变化对TCSC参数优化结果的影响并不显著.%Optimization of TCSC parameters oriented to mitigating wind farm sub-synchronous oscillations (SSO) is a strongly nonlinear parameter optimization problem, which is difficult to solve with analytic method. In this paper, nonlinear parametric optimization method, combining Simplex algorithm with electromagnetic transient simulation program (EMTP), is used to optimize TCSC parameters for suppressing wind farm SSO. The proposed method calculates objective functions using EMTP simulation results. Based on the objective functions, simplex algorithm can guide the optimization direction of TCSC parameters intelligently, find optimum parameters quickly and reliably, and reduce simulation-consuming time. Finally, in terms of wind power uncertainty, this paper preliminarily analyses the impact of wind speed change on the results of parameter optimization. It is concluded from simulation results of single-machine equivalent case that the influence of wind speed change on optimization results of TCSC parameters is not significant.

  2. 47 CFR 15.717 - TVBDs that rely on spectrum sensing.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false TVBDs that rely on spectrum sensing. 15.717... Television Band Devices § 15.717 TVBDs that rely on spectrum sensing. (a) Parties may submit applications for certification of TVBDs that rely solely on spectrum sensing to identify available channels. Devices authorized...

  3. Identification of anthropogenic and natural inputs of sulfate into a karstic coastal groundwater system in northeast China: evidence from major ions, δ13CDIC and δ34SSO4

    Science.gov (United States)

    Han, Dongmei; Song, Xianfang; Currell, Matthew J.

    2016-05-01

    The hydrogeochemical processes controlling groundwater evolution in the Daweijia area of Dalian, northeast China, were characterised using hydrochemistry and isotopes of carbon and sulfur (δ13CDIC and δ34SSO4). The aim was to distinguish anthropogenic impacts as distinct from natural processes, with a particular focus on sulfate, which is found at elevated levels (range: 54.4 to 368.8 mg L-1; mean: 174.4 mg L-1) in fresh and brackish groundwater. The current investigation reveals minor seawater intrusion impact (not exceeding 5 % of the overall solute load), in contrast with extensive impacts observed in 1982 during the height of intensive abstraction. This indicates that measures to restrict groundwater abstraction have been effective. However, hydrochemical facies analysis shows that the groundwater remains in a state of ongoing hydrochemical evolution (towards Ca-Cl type water) and quality degradation (increasing nitrate and sulfate concentrations). The wide range of NO3 concentrations (74.7-579 mg L-1) in the Quaternary aquifer indicates considerable input of fertilisers and/or leakage from septic systems. Both δ13C (-14.5 to -5.9 permil) and δ34SSO4 (+5.4 to +13.1 permil) values in groundwater show increasing trends along groundwater flow paths. While carbonate minerals may contribute to increasing δ13CDIC and δ34SSO4 values in deep karstic groundwater, high loads of agricultural fertilisers reaching the aquifer via irrigation return flow are likely the main source of the dissolved sulfate in Quaternary groundwater, as shown by distinctive isotopic ratios and a lack of evidence for other sources in the major ion chemistry. According to isotope mass balance calculations, the fertiliser contribution to overall sulfate has reached an average of 62.1 % in the Quaternary aquifer, which has a strong hydraulic connection to the underlying carbonate aquifer. The results point to an alarming level of impact from the local intensive agriculture on the groundwater

  4. Wireless Indoor Positioning Relying on Observations of Received Power and Mean Delay

    DEFF Research Database (Denmark)

    Begusic, Stjepan; Urup, Daniel Nygaard; Kolonic, Jasmina

    2013-01-01

    This contribution introduces position estimation methods relying on observations of the received power and mean delay obtained in a wideband multi-link scenario. In particular, one- and two-step methods are introduced based on statistical models of the observed link parameters. The proposed methods...... are tested on data from a wideband measurement campaign. The results show that including observations of mean delay of the wideband links can notably improve positioning accuracy as compared to relying on observations of received power alone....

  5. Reliģiskā tematika un simbolisms "Heavy Metal" subkultūrā

    OpenAIRE

    Vizla, Edmunds

    2008-01-01

    Šajā darbā tiek aplūkota Heavy Metal subkultūras attieksme pret reliģiju, tās tematiku un simboliem. Vairākās nodaļās tiek apkopoti tie galvenie simboli un tēmas, ar kurām darbojas Heavy Metal subkultūrā, izmantojot autentiskus avotus dziesmu tekstu veidā. Padziļināti tiek aplūkota kristietība, sātanisms un okultisms, senās reliģijas un pagānisms, kā arī ateisms. Viss darbs kopumā sniedz ieskatu pašā Heavy Metal subkultūras reliģiskās domāšanas iekšienē, ar spilgtiem piemēriem ilustrējot to r...

  6. Cortical control of object-specific grasp relies on adjustments of both activity and effective connectivity

    DEFF Research Database (Denmark)

    Tia, Banty; Takemi, Mitsuaki; Kosugi, Akito

    2017-01-01

    The cortical mechanisms of grasping have been extensively studied in macaques and humans. Here, we investigated whether common marmosets could rely on similar mechanisms despite striking differences in manual dexterity. Two common marmosets were trained to grasp-and-pull three objects eliciting d...

  7. 77 FR 70117 - Purchase of Certain Debt Securities by Business and Industrial Development Companies Relying on...

    Science.gov (United States)

    2012-11-23

    ... Business Administration, small entities in the financial investment industry include entities with $7... Development Companies Relying on an Investment Company Act Exemption AGENCY: Securities and Exchange... a new rule under the Investment Company Act of 1940 (``Investment Company Act'') to establish a...

  8. Bidirectional Regulation of Innate and Learned Behaviors That Rely on Frequency Discrimination by Cortical Inhibitory Neurons.

    Directory of Open Access Journals (Sweden)

    Mark Aizenberg

    2015-12-01

    Full Text Available The ability to discriminate tones of different frequencies is fundamentally important for everyday hearing. While neurons in the primary auditory cortex (AC respond differentially to tones of different frequencies, whether and how AC regulates auditory behaviors that rely on frequency discrimination remains poorly understood. Here, we find that the level of activity of inhibitory neurons in AC controls frequency specificity in innate and learned auditory behaviors that rely on frequency discrimination. Photoactivation of parvalbumin-positive interneurons (PVs improved the ability of the mouse to detect a shift in tone frequency, whereas photosuppression of PVs impaired the performance. Furthermore, photosuppression of PVs during discriminative auditory fear conditioning increased generalization of conditioned response across tone frequencies, whereas PV photoactivation preserved normal specificity of learning. The observed changes in behavioral performance were correlated with bidirectional changes in the magnitude of tone-evoked responses, consistent with predictions of a model of a coupled excitatory-inhibitory cortical network. Direct photoactivation of excitatory neurons, which did not change tone-evoked response magnitude, did not affect behavioral performance in either task. Our results identify a new function for inhibition in the auditory cortex, demonstrating that it can improve or impair acuity of innate and learned auditory behaviors that rely on frequency discrimination.

  9. Dieva koncepcija Senās Ēģiptes reliģijā

    OpenAIRE

    Lielpētere, Rasa

    2010-01-01

    Darba nosaukums ir „Dieva koncepcija senās Ēģiptes reliģijā”. Darba tēze ir: Senās Ēģiptes reliģijā valdīja monoteisms. Darba mērķis ir noskaidrot ko vairāk par kristietības saknēm – seno ēģiptiešu priekšstatu par Dievu. Darba pirmajā nodaļā sniegs ieskats Senās Ēģiptes reliģijā, kas palīdz izprast apstākļus un domāšanu Senajā Ēģiptē. Apraksts veidots, sākot no sociālā stāvokļa Senajā Ēģiptē un beidzot ar seno ēģiptiešu svētajiem rakstiem. Otrā nodaļa apspriež ēģiptologu izvir...

  10. Anemonefishes rely on visual and chemical cues to correctly identify conspecifics

    Science.gov (United States)

    Johnston, Nicole K.; Dixson, Danielle L.

    2017-09-01

    Organisms rely on sensory cues to interpret their environment and make important life-history decisions. Accurate recognition is of particular importance in diverse reef environments. Most evidence on the use of sensory cues focuses on those used in predator avoidance or habitat recognition, with little information on their role in conspecific recognition. Yet conspecific recognition is essential for life-history decisions including settlement, mate choice, and dominance interactions. Using a sensory manipulated tank and a two-chamber choice flume, anemonefish conspecific response was measured in the presence and absence of chemical and/or visual cues. Experiments were then repeated in the presence or absence of two heterospecific species to evaluate whether a heterospecific fish altered the conspecific response. Anemonefishes responded to both the visual and chemical cues of conspecifics, but relied on the combination of the two cues to recognize conspecifics inside the sensory manipulated tank. These results contrast previous studies focusing on predator detection where anemonefishes were found to compensate for the loss of one sensory cue (chemical) by utilizing a second cue (visual). This lack of sensory compensation may impact the ability of anemonefishes to acclimate to changing reef environments in the future.

  11. Poison frogs rely on experience to find the way home in the rainforest.

    Science.gov (United States)

    Pašukonis, Andrius; Warrington, Ian; Ringler, Max; Hödl, Walter

    2014-11-01

    Among vertebrates, comparable spatial learning abilities have been found in birds, mammals, turtles and fishes, but virtually nothing is known about such abilities in amphibians. Overall, amphibians are the most sedentary vertebrates, but poison frogs (Dendrobatidae) routinely shuttle tadpoles from terrestrial territories to dispersed aquatic deposition sites. We hypothesize that dendrobatid frogs rely on learning for flexible navigation. We tested the role of experience with the local cues for poison frog way-finding by (i) experimentally displacing territorial males of Allobates femoralis over several hundred metres, (ii) using a harmonic direction finder with miniature transponders to track these small frogs, and (iii) using a natural river barrier to separate the translocated frogs from any familiar landmarks. We found that homeward orientation was disrupted by the translocation to the unfamiliar area but frogs translocated over similar distances in their local area showed significant homeward orientation and returned to their territories via a direct path. We suggest that poison frogs rely on spatial learning for way-finding in their local area.

  12. Fully automatic assignment of small molecules' NMR spectra without relying on chemical shift predictions.

    Science.gov (United States)

    Castillo, Andrés M; Bernal, Andrés; Patiny, Luc; Wist, Julien

    2015-08-01

    We present a method for the automatic assignment of small molecules' NMR spectra. The method includes an automatic and novel self-consistent peak-picking routine that validates NMR peaks in each spectrum against peaks in the same or other spectra that are due to the same resonances. The auto-assignment routine used is based on branch-and-bound optimization and relies predominantly on integration and correlation data; chemical shift information may be included when available to fasten the search and shorten the list of viable assignments, but in most cases tested, it is not required in order to find the correct assignment. This automatic assignment method is implemented as a web-based tool that runs without any user input other than the acquired spectra. Copyright © 2015 John Wiley & Sons, Ltd.

  13. Logical Characterisation of Concept Transformations from Human into Machine relying on Predicate Logic

    DEFF Research Database (Denmark)

    Badie, Farshad

    2016-01-01

    Providing more human-like concept learning in machines has always been one of the most significant goals of machine learning paradigms and of human-machine interaction techniques. This article attempts to provide a logical specification of conceptual mappings from humans’ minds into machines......’ knowledge bases. I will focus on the representation of the mappings (transformations) relying on First-Order Predicate Logic. Additionally, the structure of concepts in the common ground between humans and machines will be analysed. It seems quite necessary to pay attention to the philosophy...... of constructivism and constructivist models of knowing. This research constructs a conceptual ground for expressing and analysing concepts in the common ground between humanistic and informatics sciences and in the context of human-machine interplays....

  14. Logical Characterisation of Concept Transformations from Human into Machine relying on Predicate Logic

    DEFF Research Database (Denmark)

    Badie, Farshad

    2016-01-01

    Providing more human-like concept learning in machines has always been one of the most significant goals of machine learning paradigms and of human-machine interaction techniques. This article attempts to provide a logical specification of conceptual mappings from humans’ minds into machines......’ knowledge bases. I will focus on the representation of the mappings (transformations) relying on First-Order Predicate Logic. Additionally, the structure of concepts in the common ground between humans and machines will be analysed. It seems quite necessary to pay attention to the philosophy...... of constructivism and constructivist models of knowing. This research constructs a conceptual ground for expressing and analysing concepts in the common ground between humanistic and informatics sciences and in the context of human-machine interplays....

  15. RELY: A reliability modeling system for analysis of sodium-sulfur battery configurations

    Energy Technology Data Exchange (ETDEWEB)

    Hostick, C.J.; Huber, H.D.; Doggett, W.H.; Dirks, J.A.; Dovey, J.F.; Grinde, R.B.; Littlefield, J.S.; Cuta, F.M.

    1987-06-01

    In support of the Office of Energy Storage and Distribution of the US Department of Energy (DOE), Pacific Northwest Laboratory has produced a microcomputer-based software package, called RELY, to assess the impact of sodium-sulfur cell reliability on constant current discharge battery performance. The Fortran-based software operates on IBM microcomputers and IBM-compatibles that have a minimum of 512K of internal memory. The software package has three models that provide the following: (1) a description of the failure distribution parameters used to model cell failure, (2) a Monte Carlo simulation of battery life, and (3) a detailed discharge model for a user-specified battery discharge cycle. 6 refs., 31 figs., 4 tabs.

  16. Cochlear implant users rely on tempo rather than on pitch information during perception of musical emotion.

    Science.gov (United States)

    Caldwell, Meredith; Rankin, Summer K; Jiradejvong, Patpong; Carver, Courtney; Limb, Charles J

    2015-09-01

    The purpose of this study was to investigate the extent to which cochlear implant (CI) users rely on tempo and mode in perception of musical emotion when compared with normal hearing (NH) individuals. A test battery of novel four-bar melodies was created and adapted to four permutations with alterations of tonality (major vs. minor) and tempo (presto vs. largo), resulting in non-ambiguous (major key/fast tempo and minor key/slow tempo) and ambiguous (major key/slow tempo, and minor key/fast tempo) musical stimuli. Both CI and NH participants listened to each clip and provided emotional ratings on a Likert scale of +5 (happy) to -5 (sad). A three-way ANOVA demonstrated an overall effect for tempo in both groups, and an overall effect for mode in the NH group. The CI group rated stimuli of the same tempo similarly, regardless of changes in mode, whereas the NH group did not. A subgroup analysis indicated the same effects in both musician and non-musician CI users and NH listeners. The results suggest that the CI group relied more heavily on tempo than mode in making musical emotion decisions. The subgroup analysis further suggests that level of musical training did not significantly impact this finding. CI users weigh temporal cues more heavily than pitch cues in inferring musical emotion. These findings highlight the significant disadvantage of CI users in comparison with NH listeners for music perception, particularly during recognition of musical emotion, a critically important feature of music.

  17. Pneumococcal Competence Coordination Relies on a Cell-Contact Sensing Mechanism.

    Directory of Open Access Journals (Sweden)

    Marc Prudhomme

    2016-06-01

    Full Text Available Bacteria have evolved various inducible genetic programs to face many types of stress that challenge their growth and survival. Competence is one such program. It enables genetic transformation, a major horizontal gene transfer process. Competence development in liquid cultures of Streptococcus pneumoniae is synchronized within the whole cell population. This collective behavior is known to depend on an exported signaling Competence Stimulating Peptide (CSP, whose action generates a positive feedback loop. However, it is unclear how this CSP-dependent population switch is coordinated. By monitoring spontaneous competence development in real time during growth of four distinct pneumococcal lineages, we have found that competence shift in the population relies on a self-activated cell fraction that arises via a growth time-dependent mechanism. We demonstrate that CSP remains bound to cells during this event, and conclude that the rate of competence development corresponds to the propagation of competence by contact between activated and quiescent cells. We validated this two-step cell-contact sensing mechanism by measuring competence development during co-cultivation of strains with altered capacity to produce or respond to CSP. Finally, we found that the membrane protein ComD retains the CSP, limiting its free diffusion in the medium. We propose that competence initiator cells originate stochastically in response to stress, to form a distinct subpopulation that then transmits the CSP by cell-cell contact.

  18. Frugivorous bats maintain functional habitat connectivity in agricultural landscapes but rely strongly on natural forest fragments.

    Directory of Open Access Journals (Sweden)

    Simon P Ripperger

    Full Text Available Anthropogenic changes in land use threaten biodiversity and ecosystem functioning by the conversion of natural habitat into agricultural mosaic landscapes, often with drastic consequences for the associated fauna. The first step in the development of efficient conservation plans is to understand movement of animals through complex habitat mosaics. Therefore, we studied ranging behavior and habitat use in Dermanura watsoni (Phyllostomidae, a frugivorous bat species that is a valuable seed disperser in degraded ecosystems. Radio-tracking of sixteen bats showed that the animals strongly rely on natural forest. Day roosts were exclusively located within mature forest fragments. Selection ratios showed that the bats foraged selectively within the available habitat and positively selected natural forest. However, larger daily ranges were associated with higher use of degraded habitats. Home range geometry and composition of focal foraging areas indicated that wider ranging bats performed directional foraging bouts from natural to degraded forest sites traversing the matrix over distances of up to three hundred meters. This behavior demonstrates the potential of frugivorous bats to functionally connect fragmented areas by providing ecosystem services between natural and degraded sites, and highlights the need for conservation of natural habitat patches within agricultural landscapes that meet the roosting requirements of bats.

  19. Motor imagery modulation of body sway is task-dependent and relies on imagery ability

    Directory of Open Access Journals (Sweden)

    Thiago eLemos

    2014-05-01

    Full Text Available AbstractIn this study we investigate to what extent the effects of motor imagery on postural sway are constrained by movement features and the subject’s imagery ability. Twenty-three subjects were asked to imagine three movements using the kinesthetic modality: rising on tiptoes, whole-body forward reaching, and whole-body lateral reaching. After each task, subjects reported the level of imagery vividness and were subsequently grouped into a HIGH group (scores ≥3, moderately intense imagery or a LOW group (scores ≤2, mildly intense imagery. An eyes closed trial was used as a control task. Center of gravity (COG coordinates were collected, along with surface EMG of the deltoid (medial and anterior portion and lateral gastrocnemius muscles. COG variability was quantified as the amount of fluctuations in position and velocity in the forward-backward and lateral directions. Changes in COG variability during motor imagery were observed only for the HIGH group. COG variability in the forward-backward direction was increased during the rising on tiptoes imagery, compared with the control task (p=0.01 and the lateral reaching imagery (p=0.02. Conversely, COG variability in the lateral direction was higher in rising on tiptoes and lateral reaching imagery than during the control task (p0.08 or task (p>0.46 for any of the tested muscles. In summary, motor imagery influences body sway dynamics in a task-dependent manner, and relies on the subject’ imagery ability.

  20. Organogenesis relies on SoxC transcription factors for the survival of neural and mesenchymal progenitors.

    Science.gov (United States)

    Bhattaram, Pallavi; Penzo-Méndez, Alfredo; Sock, Elisabeth; Colmenares, Clemencia; Kaneko, Kotaro J; Vassilev, Alex; Depamphilis, Melvin L; Wegner, Michael; Lefebvre, Véronique

    2010-04-12

    During organogenesis, neural and mesenchymal progenitor cells give rise to many cell lineages, but their molecular requirements for self-renewal and lineage decisions are incompletely understood. In this study, we show that their survival critically relies on the redundantly acting SoxC transcription factors Sox4, Sox11 and Sox12. The more SoxC alleles that are deleted in mouse embryos, the more severe and widespread organ hypoplasia is. SoxC triple-null embryos die at midgestation unturned and tiny, with normal patterning and lineage specification, but with massively dying neural and mesenchymal progenitor cells. Specific inactivation of SoxC genes in neural and mesenchymal cells leads to selective apoptosis of these cells, suggesting SoxC cell-autonomous roles. Tead2 functionally interacts with SoxC genes in embryonic development, and is a direct target of SoxC proteins. SoxC genes therefore ensure neural and mesenchymal progenitor cell survival, and function in part by activating this transcriptional mediator of the Hippo signalling pathway.

  1. Cell sorting enriches Escherichia coli mutants that rely on peptidoglycan endopeptidases to suppress highly aberrant morphologies.

    Science.gov (United States)

    Laubacher, Mary E; Melquist, Amy L; Chandramohan, Lakshmi; Young, Kevin D

    2013-02-01

    Bacterial morphology imparts physiological advantages to cells in different environments and, judging by the fidelity with which shape is passed to daughter cells, is a tightly regulated characteristic. Surprisingly, only in the past 10 to 15 years has significant headway been made in identifying the mechanisms by which cells create and maintain particular shapes. One reason for this is that the relevant discoveries have relied heavily on the arduous, somewhat subjective process of manual microscopy. Here, we show that flow cytometry, coupled with the sorting capability of fluorescence-activated cell sorting (FACS), can detect, quantify, and enrich bacteria with morphological alterations. The light scattering properties of several highly aberrant morphological mutants of Escherichia coli were characterized by flow cytometry. Cells from a region that overlapped the distribution of normal rod-shaped cells were collected by FACS and reincubated. After 4 to 15 iterations of this enrichment process, suppressor mutants were isolated that returned almost all the population to a near-normal shape. Suppressors were successfully isolated from strains lacking three or four penicillin binding proteins (PBPs) but not from a mutant lacking a total of seven PBPs. The peptidoglycan endopeptidase, AmpH, was identified as being important for the suppression process, as was a related endopeptidase, MepA. The results validate the use of cell sorting as a means for studying bacterial morphology and identify at least one new class of enzymes required for the suppression of cell shape defects.

  2. Sensory map transfer to the neocortex relies on pretarget ordering of thalamic axons.

    Science.gov (United States)

    Lokmane, Ludmilla; Proville, Rémi; Narboux-Nême, Nicolas; Györy, Ildiko; Keita, Maryama; Mailhes, Caroline; Léna, Clément; Gaspar, Patricia; Grosschedl, Rudolf; Garel, Sonia

    2013-05-06

    Sensory maps, such as the representation of mouse facial whiskers, are conveyed throughout the nervous system by topographic axonal projections that preserve neighboring relationships between adjacent neurons. In particular, the map transfer to the neocortex is ensured by thalamocortical axons (TCAs), whose terminals are topographically organized in response to intrinsic cortical signals. However, TCAs already show a topographic order early in development, as they navigate toward their target. Here, we show that this preordering of TCAs is required for the transfer of the whisker map to the neocortex. Using Ebf1 conditional inactivation that specifically perturbs the development of an intermediate target, the basal ganglia, we scrambled TCA topography en route to the neocortex without affecting the thalamus or neocortex. Notably, embryonic somatosensory TCAs were shifted toward the visual cortex and showed a substantial intermixing along their trajectory. Somatosensory TCAs rewired postnatally to reach the somatosensory cortex but failed to form a topographic anatomical or functional map. Our study reveals that sensory map transfer relies not only on positional information in the projecting and target structures but also on preordering of axons along their trajectory, thereby opening novel perspectives on brain wiring.

  3. Regulatory theory: commercially sustainable markets rely upon satisfying the public interest in obtaining credible goods.

    Science.gov (United States)

    Warren-Jones, Amanda

    2017-10-01

    Regulatory theory is premised on the failure of markets, prompting a focus on regulators and industry from economic perspectives. This article argues that overlooking the public interest in the sustainability of commercial markets risks markets failing completely. This point is exemplified through health care markets - meeting an essential need - and focuses upon innovative medicines as the most desired products in that market. If this seemingly invulnerable market risks failure, there is a pressing need to consider the public interest in sustainable markets within regulatory literature and practice. Innovative medicines are credence goods, meaning that the sustainability of the market fundamentally relies upon the public trusting regulators to vouch for product quality. Yet, quality is being eroded by patent bodies focused on economic benefits from market growth, rather than ensuring innovatory value. Remunerative bodies are not funding medicines relative to market value, and market authorisation bodies are not vouching for robust safety standards or confining market entry to products for 'unmet medical need'. Arguably, this failure to assure quality heightens the risk of the market failing where it cannot be substituted by the reputation or credibility of providers of goods and/or information such as health care professionals/institutions, patient groups or industry.

  4. Quality of record linkage in a highly automated cancer registry that relies on encrypted identity data

    Directory of Open Access Journals (Sweden)

    Schmidtmann, Irene

    2016-06-01

    Full Text Available Objectives: In the absence of unique ID numbers, cancer and other registries in Germany and elsewhere rely on identity data to link records pertaining to the same patient. These data are often encrypted to ensure privacy. Some record linkage errors unavoidably occur. These errors were quantified for the cancer registry of North Rhine Westphalia which uses encrypted identity data. Methods: A sample of records was drawn from the registry, record linkage information was included. In parallel, plain text data for these records were retrieved to generate a gold standard. Record linkage error frequencies in the cancer registry were determined by comparison of the results of the routine linkage with the gold standard. Error rates were projected to larger registries.Results: In the sample studied, the homonym error rate was 0.015%; the synonym error rate was 0.2%. The F-measure was 0.9921. Projection to larger databases indicated that for a realistic development the homonym error rate will be around 1%, the synonym error rate around 2%.Conclusion: Observed error rates are low. This shows that effective methods to standardize and improve the quality of the input data have been implemented. This is crucial to keep error rates low when the registry’s database grows. The planned inclusion of unique health insurance numbers is likely to further improve record linkage quality. Cancer registration entirely based on electronic notification of records can process large amounts of data with high quality of record linkage.

  5. Secreted autotransporter toxin (Sat) triggers autophagy in epithelial cells that relies on cell detachment.

    Science.gov (United States)

    Liévin-Le Moal, Vanessa; Comenge, Yannick; Ruby, Vincent; Amsellem, Raymonde; Nicolas, Valérie; Servin, Alain L

    2011-07-01

    The secreted autotransporter toxin, Sat, which belongs to the subfamily of serine protease autotransporters of Enterobacteriaceae, acts as a virulence factor in extraintestinal and intestinal pathogenic strains of Escherichia coli. We observed that HeLa cells exposed to the cell-free culture supernatant of recombinant strain AAEC185p(Sat-IH11128) producing the Sat toxin (CFCS(Sat) ), displayed dramatic disorganization of the F-actin cytoskeleton before loosening cell-to-cell junctions and detachment. Examination of the effect of Sat on GFP-microtubule-associated protein light chain 3 (LC3) HeLa cells revealed that CFCS(Sat) -induced autophagy follows CFCS(Sat) -induced F-actin cytoskeleton rearrangement. The induced autophagy shows an acceleration of the autophagy flux soon after Sat treatment, followed later by a blockade of the flux leading to the accumulation of large GFP-LC3-positive vacuoles in the cell cytoplasm. CFCS(Sat) did not induce cell detachment in autophagy-deficient mouse embryonic fibroblasts in contrast with wild-type mouse embryonic fibroblasts. The CFCS(Sat) -induced large GFP-LC3 dots do not display the characteristics of autophagolysosomes including expression of cathepsin D and Lamp-1 and 2 proteins, and Lysotracker Red- and DQ-BSA-positive labelling. We provide evidences that CFCS(Sat) -induced autophagy is not a cell response intended to get rid of the intracellular toxin. By a pharmacological blockers approach, we found that the blockade of Erk1/2 and p38 MAPKs, but not JNK, inhibited the CFCS(Sat) -induced autophagy and cell detachment whereas phosphatidylinositol-3 kinase blockers inhibiting canonical autophagy were inactive. When attached CFCS(Sat) -treated cells start to detach they showed caspase-independent cell death and rearrangements of the focal adhesion-associated vinculin and paxillin. Collectively, our results support that Sat triggers autophagy in epithelial cells that relies on its cell-detachment effect. © 2011 Blackwell

  6. Traceable quantum sensing and metrology relied up a quantum electrical triangle principle

    Science.gov (United States)

    Fang, Yan; Wang, Hengliang; Yang, Xinju; Wei, Jingsong

    2016-11-01

    Hybrid quantum state engineering in quantum communication and imaging1-2 needs traceable quantum sensing and metrology, which are especially critical to quantum internet3 and precision measurements4 that are important across all fields of science and technology-. We aim to set up a mode of traceable quantum sensing and metrology. We developed a method by specially transforming an atomic force microscopy (AFM) and a scanning tunneling microscopy (STM) into a conducting atomic force microscopy (C-AFM) with a feedback control loop, wherein quantum entanglement enabling higher precision was relied upon a set-point, a visible light laser beam-controlled an interferometer with a surface standard at z axis, diffractometers with lateral standards at x-y axes, four-quadrant photodiode detectors, a scanner and its image software, a phase-locked pre-amplifier, a cantilever with a kHz Pt/Au conducting tip, a double barrier tunneling junction model, a STM circuit by frequency modulation and a quantum electrical triangle principle involving single electron tunneling effect, quantum Hall effect and Josephson effect5. The average and standard deviation result of repeated measurements on a 1 nm height local micro-region of nanomedicine crystal hybrid quantum state engineering surface and its differential pA level current and voltage (dI/dV) in time domains by using C-AFM was converted into an international system of units: Siemens (S), an indicated value 0.86×10-12 S (n=6) of a relative standard uncertainty was superior over a relative standard uncertainty reference value 2.3×10-10 S of 2012 CODADA quantized conductance6. It is concluded that traceable quantum sensing and metrology is emerging.

  7. High sugar-induced insulin resistance in Drosophila relies on the lipocalin Neural Lazarillo.

    Directory of Open Access Journals (Sweden)

    Matthieu Y Pasco

    Full Text Available In multicellular organisms, insulin/IGF signaling (IIS plays a central role in matching energy needs with uptake and storage, participating in functions as diverse as metabolic homeostasis, growth, reproduction and ageing. In mammals, this pleiotropy of action relies in part on a dichotomy of action of insulin, IGF-I and their respective membrane-bound receptors. In organisms with simpler IIS, this functional separation is questionable. In Drosophila IIS consists of several insulin-like peptides called Dilps, activating a unique membrane receptor and its downstream signaling cascade. During larval development, IIS is involved in metabolic homeostasis and growth. We have used feeding conditions (high sugar diet, HSD that induce an important change in metabolic homeostasis to monitor possible effects on growth. Unexpectedly we observed that HSD-fed animals exhibited severe growth inhibition as a consequence of peripheral Dilp resistance. Dilp-resistant animals present several metabolic disorders similar to those observed in type II diabetes (T2D patients. By exploring the molecular mechanisms involved in Drosophila Dilp resistance, we found a major role for the lipocalin Neural Lazarillo (NLaz, a target of JNK signaling. NLaz expression is strongly increased upon HSD and animals heterozygous for an NLaz null mutation are fully protected from HSD-induced Dilp resistance. NLaz is a secreted protein homologous to the Retinol-Binding Protein 4 involved in the onset of T2D in human and mice. These results indicate that insulin resistance shares common molecular mechanisms in flies and human and that Drosophila could emerge as a powerful genetic system to study some aspects of this complex syndrome.

  8. High sugar-induced insulin resistance in Drosophila relies on the lipocalin Neural Lazarillo.

    Science.gov (United States)

    Pasco, Matthieu Y; Léopold, Pierre

    2012-01-01

    In multicellular organisms, insulin/IGF signaling (IIS) plays a central role in matching energy needs with uptake and storage, participating in functions as diverse as metabolic homeostasis, growth, reproduction and ageing. In mammals, this pleiotropy of action relies in part on a dichotomy of action of insulin, IGF-I and their respective membrane-bound receptors. In organisms with simpler IIS, this functional separation is questionable. In Drosophila IIS consists of several insulin-like peptides called Dilps, activating a unique membrane receptor and its downstream signaling cascade. During larval development, IIS is involved in metabolic homeostasis and growth. We have used feeding conditions (high sugar diet, HSD) that induce an important change in metabolic homeostasis to monitor possible effects on growth. Unexpectedly we observed that HSD-fed animals exhibited severe growth inhibition as a consequence of peripheral Dilp resistance. Dilp-resistant animals present several metabolic disorders similar to those observed in type II diabetes (T2D) patients. By exploring the molecular mechanisms involved in Drosophila Dilp resistance, we found a major role for the lipocalin Neural Lazarillo (NLaz), a target of JNK signaling. NLaz expression is strongly increased upon HSD and animals heterozygous for an NLaz null mutation are fully protected from HSD-induced Dilp resistance. NLaz is a secreted protein homologous to the Retinol-Binding Protein 4 involved in the onset of T2D in human and mice. These results indicate that insulin resistance shares common molecular mechanisms in flies and human and that Drosophila could emerge as a powerful genetic system to study some aspects of this complex syndrome.

  9. Fruit production in three masting tree species does not rely on stored carbon reserves.

    Science.gov (United States)

    Hoch, Günter; Siegwolf, Rolf T W; Keel, Sonja G; Körner, Christian; Han, Qingmin

    2013-03-01

    Fruiting is typically considered to massively burden the seasonal carbon budget of trees. The cost of reproduction has therefore been suggested as a proximate factor explaining observed mast-fruiting patterns. Here, we used a large-scale, continuous (13)C labeling of mature, deciduous trees in a temperate Swiss forest to investigate to what extent fruit formation in three species with masting reproduction behavior (Carpinus betulus, Fagus sylvatica, Quercus petraea) relies on the import of stored carbon reserves. Using a free-air CO2 enrichment system, we exposed trees to (13)C-depleted CO2 during 8 consecutive years. By the end of this experiment, carbon reserve pools had significantly lower δ(13)C values compared to control trees. δ(13)C analysis of new biomass during the first season after termination of the CO2 enrichment allowed us to distinguish the sources of built-in carbon (old carbon reserves vs. current assimilates). Flowers and expanding leaves carried a significant (13)C label from old carbon stores. In contrast, fruits and vegetative infructescence tissues were exclusively produced from current, unlabeled photoassimilates in all three species, including F. sylvatica, which had a strong masting season. Analyses of δ(13)C in purified starch from xylem of fruit-bearing shoots revealed a complete turn-over of starch during the season, likely due to its usage for bud break. This study is the first to directly demonstrate that fruiting is independent from old carbon reserves in masting trees, with significant implications for mechanistic models that explain mast seeding.

  10. You can't rely on color, yet we all do 2.0 (Manuscript Only)

    Science.gov (United States)

    van Nes, Floris L.

    2014-01-01

    Everybody views and uses color from early childhood onwards. But this magnificent property of all objects around us turns out to be elusive if you try to specify it and communicate it to another person. Also, people often don't know what effects color may have under different conditions. However, color is so important and omnipresent, that people can hardly avoid to 'rely on it' - so they do, in particular on its predictability. Thus, there is a discrepancy between the seeming self-evidence of color and the difficulty in specifying it accurately, for the prevailing circumstances. In order to analyze this situation, and possibly remedy it, a short historic perspective of the utilization and specification of color is given. The 'utilization' includes the emotional effects of color, which are important in, for instance, interior decorating but also play a role in literature and religion. 'Specification' begins with the early efforts by scientists, philosophers and artists to bring some order and understanding in what was observed with and while using color. Color has a number of basic functions: embellishment; attracting attention; coding; and bringing order in text by causing text parts presented in the same color to be judged as belonging together. People with a profession that involves color choices for many others, such as designers and manufacturers of products, including electronic visual displays, should have a fairly thorough knowledge of colorimetry and color perception. Unfortunately, they often don't, simply because for 'practitioners' whose work involves different aspects, applying color being only one of those, the available tools for specifying and applying color turn out to be too difficult to use. Two consequences of an insufficient knowledge of the effects color may have are given here. The first of these consequences, on color blindness, relates to 8% of the population, but the second one, on reading colored text, bears on everyone. Practical

  11. Optimization of cDNA microarrays procedures using criteria that do not rely on external standards

    Directory of Open Access Journals (Sweden)

    Beisvag Vidar

    2007-10-01

    Full Text Available Abstract Background The measurement of gene expression using microarray technology is a complicated process in which a large number of factors can be varied. Due to the lack of standard calibration samples such as are used in traditional chemical analysis it may be a problem to evaluate whether changes done to the microarray procedure actually improve the identification of truly differentially expressed genes. The purpose of the present work is to report the optimization of several steps in the microarray process both in laboratory practices and in data processing using criteria that do not rely on external standards. Results We performed a cDNA microarry experiment including RNA from samples with high expected differential gene expression termed "high contrasts" (rat cell lines AR42J and NRK52E compared to self-self hybridization, and optimized a pipeline to maximize the number of genes found to be differentially expressed in the "high contrasts" RNA samples by estimating the false discovery rate (FDR using a null distribution obtained from the self-self experiment. The proposed high-contrast versus self-self method (HCSSM requires only four microarrays per evaluation. The effects of blocking reagent dose, filtering, and background corrections methodologies were investigated. In our experiments a dose of 250 ng LNA (locked nucleic acid dT blocker, no background correction and weight based filtering gave the largest number of differentially expressed genes. The choice of background correction method had a stronger impact on the estimated number of differentially expressed genes than the choice of filtering method. Cross platform microarray (Illumina analysis was used to validate that the increase in the number of differentially expressed genes found by HCSSM was real. Conclusion The results show that HCSSM can be a useful and simple approach to optimize microarray procedures without including external standards. Our optimizing method is highly

  12. The Long-Term Multicenter Observational Study of Dabigatran Treatment in Patients With Atrial Fibrillation (RELY-ABLE) Study

    DEFF Research Database (Denmark)

    Connolly, S. J.; Wallentin, L.; Ezekowitz, M. D.

    2013-01-01

    Background During follow-up of between 1 and 3 years in the Randomized Evaluation of Long-term Anticoagulation Therapy (RE-LY) trial, 2 doses of dabigatran etexilate were shown to be effective and safe for the prevention of stroke or systemic embolism in patients with atrial fibrillation. There i.......3 years of continued treatment with dabigatran after RE-LY, there was a higher rate of major bleeding with dabigatran 150 mg twice daily in comparison with 110 mg, and similar rates of stroke and death. Clinical Trial Registration URL: . Unique identifier: NCT00808067....

  13. Anemia predicts thromboembolic events, bleeding complications and mortality in patients with atrial fibrillation : insights from the RE-LY trial

    NARCIS (Netherlands)

    Westenbrink, B. D.; Alings, M.; Connolly, S. J.; Eikelboom, J.; Ezekowitz, M. D.; Oldgren, J.; Yang, S.; Pongue, J.; Yusuf, S.; Wallentin, L.; van Gilst, W. H.

    2015-01-01

    BackgroundAnemia may predispose to thromboembolic events or bleeding in anticoagulated patients with atrial fibrillation (AF). ObjectivesTo investigate whether anemia is associated with thromboembolic events and bleeding in patients with AF. Patients and methodsWe retrospectively analyzed the RE-LY

  14. Anemia predicts thromboembolic events, bleeding complications and mortality in patients with atrial fibrillation : insights from the RE-LY trial

    NARCIS (Netherlands)

    Westenbrink, B. D.; Alings, M.; Connolly, S. J.; Eikelboom, J.; Ezekowitz, M. D.; Oldgren, J.; Yang, S.; Pongue, J.; Yusuf, S.; Wallentin, L.; van Gilst, W. H.

    BackgroundAnemia may predispose to thromboembolic events or bleeding in anticoagulated patients with atrial fibrillation (AF). ObjectivesTo investigate whether anemia is associated with thromboembolic events and bleeding in patients with AF. Patients and methodsWe retrospectively analyzed the RE-LY

  15. 16 CFR 1115.5 - Reporting of failures to comply with a voluntary consumer product safety standard relied upon by...

    Science.gov (United States)

    2010-01-01

    ... voluntary consumer product safety standard relied upon by the Commission under section 9 of the CPSA. 1115.5 Section 1115.5 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS SUBSTANTIAL PRODUCT HAZARD REPORTS General Interpretation § 1115.5 Reporting of failures to...

  16. Immobilization of α-Amylase from Anoxybacillus sp. SK3-4 on ReliZyme and Immobead Supports

    Directory of Open Access Journals (Sweden)

    Ummirul Mukminin Kahar

    2016-09-01

    Full Text Available α-Amylase from Anoxybacillus sp. SK3-4 (ASKA is a thermostable enzyme that produces a high level of maltose from starches. A truncated ASKA (TASKA variant with improved expression and purification efficiency was characterized in an earlier study. In this work, TASKA was purified and immobilized through covalent attachment on three epoxide (ReliZyme EP403/M, Immobead IB-150P, and Immobead IB-150A and an amino-epoxide (ReliZyme HFA403/M activated supports. Several parameters affecting immobilization were analyzed, including the pH, temperature, and quantity (mg of enzyme added per gram of support. The influence of the carrier surface properties, pore sizes, and lengths of spacer arms (functional groups on biocatalyst performances were studied. Free and immobilized TASKAs were stable at pH 6.0–9.0 and active at pH 8.0. The enzyme showed optimal activity and considerable stability at 60 °C. Immobilized TASKA retained 50% of its initial activity after 5–12 cycles of reuse. Upon degradation of starches and amylose, only immobilized TASKA on ReliZyme HFA403/M has comparable hydrolytic ability with the free enzyme. To the best of our knowledge, this is the first report of an immobilization study of an α-amylase from Anoxybacillus spp. and the first report of α-amylase immobilization using ReliZyme and Immobeads as supports.

  17. 78 FR 29392 - Embedded Digital Devices in Safety-Related Systems, Systems Important to Safety, and Items Relied...

    Science.gov (United States)

    2013-05-20

    ... COMMISSION Embedded Digital Devices in Safety-Related Systems, Systems Important to Safety, and Items Relied... Regulatory Issue Summary (RIS) 2013-XX, ``Embedded Digital Devices in Safety-Related Systems, Systems... basic components with embedded digital devices. DATES: Submit comments by July 19, 2013....

  18. Analysis of complete genomes suggests that many prokaryotes do not rely on hairpin formation in transcription termination.

    OpenAIRE

    Washio, T.; Sasayama, J; Tomita, M.

    1998-01-01

    Free energy values of mRNA tertiary structures around stop codons were systematically calculated to surmise the hairpin-forming potential for all genes in each of the 16 complete prokaryote genomes. Instead of trying to detect each individual hairpin, we averaged the free energy values around the stop codons over the entire genome to predict how extensively the organism relies on hairpin formation in the process of transcription termination. The free energy values of Escherichia coli K-12 sho...

  19. Flower bats (Glossophaga soricina and fruit bats (Carollia perspicillata rely on spatial cues over shapes and scents when relocating food.

    Directory of Open Access Journals (Sweden)

    Gerald G Carter

    Full Text Available BACKGROUND: Natural selection can shape specific cognitive abilities and the extent to which a given species relies on various cues when learning associations between stimuli and rewards. Because the flower bat Glossophaga soricina feeds primarily on nectar, and the locations of nectar-producing flowers remain constant, G. soricina might be predisposed to learn to associate food with locations. Indeed, G. soricina has been observed to rely far more heavily on spatial cues than on shape cues when relocating food, and to learn poorly when shape alone provides a reliable cue to the presence of food. METHODOLOGY/PRINCIPAL FINDINGS: Here we determined whether G. soricina would learn to use scent cues as indicators of the presence of food when such cues were also available. Nectar-producing plants fed upon by G. soricina often produce distinct, intense odors. We therefore expected G. soricina to relocate food sources using scent cues, particularly the flower-produced compound, dimethyl disulfide, which is attractive even to G. soricina with no previous experience of it. We also compared the learning of associations between cues and food sources by G. soricina with that of a related fruit-eating bat, Carollia perspicillata. We found that (1 G. soricina did not learn to associate scent cues, including dimethyl disulfide, with feeding sites when the previously rewarded spatial cues were also available, and (2 both the fruit-eating C. perspicillata and the flower-feeding G. soricina were significantly more reliant on spatial cues than associated sensory cues for relocating food. CONCLUSIONS/SIGNIFICANCE: These findings, taken together with past results, provide evidence of a powerful, experience-independent predilection of both species to rely on spatial cues when attempting to relocate food.

  20. Relying on known or exploring for new? Movement patterns and reproductive resource use in a tadpole-transporting frog

    Directory of Open Access Journals (Sweden)

    Kristina B. Beck

    2017-08-01

    Full Text Available Animals relying on uncertain, ephemeral and patchy resources have to regularly update their information about profitable sites. For many tropical amphibians, widespread, scattered breeding pools constitute such fluctuating resources. Among tropical amphibians, poison frogs (Dendrobatidae exhibit some of the most complex spatial and parental behaviors—including territoriality and tadpole transport from terrestrial clutches to ephemeral aquatic deposition sites. Recent studies have revealed that poison frogs rely on spatial memory to successfully navigate through their environment. This raises the question of when and how these frogs gain information about the area and suitable reproductive resources. To investigate the spatial patterns of pool use and to reveal potential explorative behavior, we used telemetry to follow males of the territorial dendrobatid frog Allobates femoralis during tadpole transport and subsequent homing. To elicit exploration, we reduced resource availability experimentally by simulating desiccated deposition sites. We found that tadpole transport is strongly directed towards known deposition sites and that frogs take similar direct paths when returning to their home territory. Frogs move faster during tadpole transport than when homing after the deposition, which probably reflects different risks and costs during these two movement phases. We found no evidence for exploration, neither during transport nor homing, and independent of the availability of deposition sites. We suggest that prospecting during tadpole transport is too risky for the transported offspring as well as for the transporting male. Relying on spatial memory of multiple previously discovered pools appears to be the predominant and successful strategy for the exploitation of reproductive resources in A. femoralis. Our study provides for the first time a detailed description of poison frog movement patterns during tadpole transport and corroborates

  1. The immune toxicity of titanium dioxide on primary pulmonary alveolar macrophages relies on their surface area and crystal structure.

    Science.gov (United States)

    Liu, Ran; Yin, Li-hong; Pu, Yue-pu; Li, Yun-hui; Zhang, Xiao-qiang; Liang, Ge-yu; Li, Xiao-bo; Zhang, Juan; Li, Yan-fen; Zhang, Xue-yan

    2010-12-01

    Surface properties are critical to assess effects of titanium dioxide (TiO2) primary nanoparticles on the immune function of pulmonary alveolar macrophage (PAMs). In this study the immune toxicity of TiO2 primary nanoparticles on PAMs relies on their surface area and crystal structure were determined. The primary PAMs of rats exposed to different sizes and crystal structure of TiO2 particles at different dosages for 24 hrs were evaluated for cytokines, phagocytosis, chemotaxis and surface molecules expression. Nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) level of PAMs significantly increased when exposed to TiO2 primary particles and there were significant association with the exposure total surface area and crystal structure of TiO2 particles in the former. TiO2 particles showed significant inhibiting effects on phagocytotic ability, chemotactic ability, Fc receptors and MHC-II molecular expression of macrophages compared with control. Exposure dosage and crystal structure of TiO2 particles play effects on phagocytotic ability and chemotactic ability of PAMs. These results suggested that TiO2 nanoparticles could induce the release of inflammatory mediators, initiate the inflammation development and inhibit the immune function of PAMs associated with non-specific immunity and specific immunity relies on surface area and crystal structure. NO activity might be a candidate marker indicating the TiO2 exposure burden and cell damage in PAMs.

  2. Episodic feeling-of-knowing relies on noncriterial recollection and familiarity: Evidence using an online remember-know procedure.

    Science.gov (United States)

    Isingrini, Michel; Sacher, Mathilde; Perrotin, Audrey; Taconnat, Laurence; Souchay, Céline; Stoehr, Hélène; Bouazzaoui, Badiâa

    2016-04-01

    We examined the hypothesis that feeling-of-knowing judgments rely on recollection as well as on familiarity prompted by the cue presentation. A remember-know-no memory procedure was combined with the episodic FOK procedure employing a cue-target pair memory task. The magnitude of FOK judgments and FOK accuracy were examined as a function of recollection, familiarity, or the "no memory" option. Results showed that the proportion of R and K responses was similar. FOK accuracy and magnitude of FOK judgments were higher for R and K responses than for N responses. FOK accuracy related to R and K responses were above chance level, but FOK was not accurate in the "no memory" condition. Finally, both FOK magnitude and FOK accuracy were related more to recollection than to familiarity. These results support the hypothesis that both recollection and familiarity are determinants of the FOK process, although they suggest that recollection has a stronger influence.

  3. Optimal myelin elongation relies on YAP activation by axonal growth and inhibition by Crb3/Hippo pathway

    Science.gov (United States)

    Fernando, Ruani N.; Cotter, Laurent; Perrin-Tricaud, Claire; Berthelot, Jade; Bartolami, Sylvain; Pereira, Jorge A.; Gonzalez, Sergio; Suter, Ueli; Tricaud, Nicolas

    2016-01-01

    Fast nerve conduction relies on successive myelin segments that electrically isolate axons. Segment geometry—diameter and length—is critical for the optimization of nerve conduction and the molecular mechanisms allowing this optimized geometry are partially known. We show here that peripheral myelin elongation is dynamically regulated by stimulation of YAP (Yes-associated protein) transcription cofactor activity during axonal elongation and limited by inhibition of YAP activity via the Hippo pathway. YAP promotes myelin and non-myelin genes transcription while the polarity protein Crb3, localized at the tips of the myelin sheath, activates the Hippo pathway to temper YAP activity, therefore allowing for optimal myelin growth. Dystrophic Dy2j/2j mice mimicking human peripheral neuropathy with reduced internodal lengths have decreased nuclear YAP which, when corrected, leads to longer internodes. These data show a novel mechanism controlling myelin growth and nerve conduction, and provide a molecular ground for disease with short myelin segments. PMID:27435623

  4. Estimation of the Patients' Adaptation to Noble Alloy Dentures Relying on the Parameters of Biological Fluids in Oral Cavities

    Institute of Scientific and Technical Information of China (English)

    LEBEDENKO I. Yu.; PARUNOV V. A.; KITKINA T. B.

    2012-01-01

    For the study of the effect of Plagodent and Palladent noble alloy dentures (OJSC "SIC ‘Supermetal’",Russia),the elemental compositions of the fluids obtained from gingival sulcus of abutment teeth of metal-ceramic dentures with frames made of the above-stated dental alloys,have been investigated.Response of white blood cells and fibroblasts in the gingival fluid and the mixed saliva of the patients a long time after prosthetic repair,relying on the content of proinflammatory interleukins IL-1 β and IL-6,anti-inflammatory interleukins IL-4 and IL-10,the factor of tumor necrosis TNF-α and lactoferrin,has been investigated.The results obtained have convincingly proved the biosafety of the Plagodent and Palladent noble alloys.

  5. Influence of Computerized Sounding Out on Spelling Performance for Children who do and not rely on AAC

    Science.gov (United States)

    McCarthy, Jillian H.; Hogan, Tiffany P.; Beukelman, David R.; Schwarz, Ilsa E.

    2015-01-01

    Purpose Spelling is an important skill for individuals who rely on augmentative alternative communication (AAC). The purpose of this study was to investigate how computerized sounding out influenced spelling accuracy of pseudo-words. Computerized sounding out was defined as a word elongated, thus providing an opportunity for a child to hear all the sounds in the word at a slower rate. Methods Seven children with cerebral palsy, four who use AAC and three who do not, participated in a single subject AB design. Results The results of the study indicated that the use of computerized sounding out increased the phonologic accuracy of the pseudo-words produced by participants. Conclusion The study provides preliminary evidence for the use of computerized sounding out during spelling tasks for children with cerebral palsy who do and do not use AAC. Future directions and clinical implications are discussed. PMID:24512195

  6. Can we rely on the antiretroviral treatment as the only means for human immunodeficiency virusprevention? A Public Health perspective.

    Science.gov (United States)

    Mozalevskis, Antons; Manzanares-Laya, Sandra; García de Olalla, Patricia; Moreno, Antonio; Jacques-Aviñó, Constanza; Caylà, Joan A

    2015-11-01

    The evidence that supports the preventive effect of combination antiretroviral treatment (cART) in HIV sexual transmission suggested the so-called 'treatment as prevention' (TAP) strategy as a promising tool for slowing down HIV transmission. As the messages and attitudes towards condom use in the context of TAP appear to be somehow confusing, the aim here is to assess whether relying on cART alone to prevent HIV transmission can currently be recommended from the Public Health perspective. A review is made of the literature on the effects of TAP strategy on HIV transmission and the epidemiology of other sexual transmitted infections (STIs) in the cART era, and recommendations from Public Health institutions on the TAP as of February 2014. The evolution of HIV and other STIs in Barcelona from 2007 to 2012 has also been analysed. Given that the widespread use of cART has coincided with an increasing incidence of HIV and other STIs, mainly amongst men who have sex with men, a combination and diversified prevention methods should always be considered and recommended in counselling. An informed decision on whether to stop using condoms should only be made by partners within stable couples, and after receiving all the up-to-date information regarding TAP. From the public health perspective, primary prevention should be a priority; therefore relying on cART alone is not a sufficient strategy to prevent new HIV and other STIs. Copyright © 2014 Elsevier España, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

  7. 49 CFR 40.371 - On what information does an initiating official rely in deciding whether to start a PIE proceeding?

    Science.gov (United States)

    2010-10-01

    ... rely in deciding whether to start a PIE proceeding? 40.371 Section 40.371 Transportation Office of the... start a PIE proceeding? (a) An initiating official may rely on credible information from any source as the basis for starting a PIE proceeding. (b) Before sending a correction notice (see § 40.373),...

  8. 17 CFR 270.12d1-3 - Exemptions for investment companies relying on section 12(d)(1)(F) of the Act.

    Science.gov (United States)

    2010-04-01

    ...) Exemption from sales charge limits. A registered investment company (“acquiring fund”) that relies on... company (“acquired fund”) may offer or sell any security it issues through a principal underwriter or... companies relying on section 12(d)(1)(F) of the Act. 270.12d1-3 Section 270.12d1-3 Commodity and...

  9. BÜSYAT Periodicals Retrieval System BÜSYAT Süreli Yayın Sorgulama Sistemi

    Directory of Open Access Journals (Sweden)

    Meltem Özturan

    1995-06-01

    Full Text Available Nowadays it gets more difficult to access information in periodicals due to the increase in the number of these periodicals. But, on the other hand, computers are widely used today in different fields to complete jobs easily and efficiently. One of those applications is the easy and quick access to the required information in periodicals. User interfaces that form the bridge between human and machine undertake a critical role in access operations. Though as an extension of ergonomy discipline, a great experience of user interface design has been accumulated, still there would be some new system-specific design criteria for each new application. In this paper, a periodicals retrieval system and a software called BÜSYAT, designed and developed to take into account of the above mentioned considerations, are discussed and presented. Günümüzde sayısı gittikçe artmakta olan süreli yayınlarda istenen bilgiye erişim gitgide zorlaşmaktadır. Ancak, diğer taraftan hızlandırıcı ve kolaylaştırıcı özelliği olan bilgisayarlar pek çok alanda kullanılmaktadır. Söz konusu uygulamalardan birisi de süreli yayınlarda istenen bilgiye kolay ve hızlı erişimin sağlanabilmesidir. Bilgisayarlar ile kullanıcılar arasındaki bağlantıyı kuran kullanıcı arayüzleri ise bilgiye erişim uygulamalarında kritik bir yol üstlenmektedir. Her ne kadar ergonomi disiplininin uzantısı olarak son yıllarda bilgisayar programlarında arayüzü tasarımı konusunda büyük bir birikim olmuşsa da, yine de her program için kendi özgün koşullarına bağlı olarak yeni bir tasarım çalışması gerekmektedir. Bu makalede, yukarıda belirtilen hususlar doğrultusunda, süreli yayınlarda bilgi erişim amaçlı tasarlanmış bir sistem ve bu tasarım doğrultusunda geliştirilmiş BÜSYAT adlı yazılım tartışılmakta ve tanıtılmaktadır.

  10. Social Plasticity Relies on Different Neuroplasticity Mechanisms across the Brain Social Decision-Making Network in Zebrafish

    Science.gov (United States)

    Teles, Magda C.; Cardoso, Sara D.; Oliveira, Rui F.

    2016-01-01

    Social living animals need to adjust the expression of their behavior to their status within the group and to changes in social context and this ability (social plasticity) has an impact on their Darwinian fitness. At the proximate level social plasticity must rely on neuroplasticity in the brain social decision-making network (SDMN) that underlies the expression of social behavior, such that the same neural circuit may underlie the expression of different behaviors depending on social context. Here we tested this hypothesis in zebrafish by characterizing the gene expression response in the SDMN to changes in social status of a set of genes involved in different types of neural plasticity: bdnf, involved in changes in synaptic strength; npas4, involved in contextual learning and dependent establishment of GABAergic synapses; neuroligins (nlgn1 and nlgn2) as synaptogenesis markers; and genes involved in adult neurogenesis (wnt3 and neurod). Four social phenotypes were experimentally induced: Winners and Losers of a real-opponent interaction; Mirror-fighters, that fight their own image in a mirror and thus do not experience a change in social status despite the expression of aggressive behavior; and non-interacting fish, which were used as a reference group. Our results show that each social phenotype (i.e., Winners, Losers, and Mirror-fighters) present specific patterns of gene expression across the SDMN, and that different neuroplasticity genes are differentially expressed in different nodes of the network (e.g., BDNF in the dorsolateral telencephalon, which is a putative teleost homolog of the mammalian hippocampus). Winners expressed unique patterns of gene co-expression across the SDMN, whereas in Losers and Mirror-fighters the co-expression patterns were similar in the dorsal regions of the telencephalon and in the supracommissural nucleus of the ventral telencephalic area, but differents in the remaining regions of the ventral telencephalon. These results

  11. Analysis of complete genomes suggests that many prokaryotes do not rely on hairpin formation in transcription termination.

    Science.gov (United States)

    Washio, T; Sasayama, J; Tomita, M

    1998-12-01

    Free energy values of mRNA tertiary structures around stop codons were systematically calculated to surmise the hairpin-forming potential for all genes in each of the 16 complete prokaryote genomes. Instead of trying to detect each individual hairpin, we averaged the free energy values around the stop codons over the entire genome to predict how extensively the organism relies on hairpin formation in the process of transcription termination. The free energy values of Escherichia coli K-12 shows a sharp drop, as expected, at 30 bp downstream of the stop codon, presumably due to hairpin-forming sequences. Similar drops are observed for Haemophilus influenzae Rd, Bacillus subtilis and Chlamydia trachomatis, suggesting that these organisms also form hairpins at their transcription termination sites. On the other hand, 12 other prokaryotes- Mycoplasma genitalium, Mycoplasma pneumoniae, Synechocystis PCC6803, Helicobacter pylori, Borrelia burgdorferi, Methanococcus jannaschii, Archaeoglobus fulgidus, Methanobacterium thermoautotrophicum, Aquifex aeolicus, Pyrococcus horikoshii, Mycobacterium tuberculosis and Treponema pallidum -show no apparent decrease in free energy value at the corresponding regions. This result suggests that these prokaryotes, or at least some of them, may never form hairpins at their transcription termination sites.

  12. A second mechanism for aluminum resistance in wheat relies on the constitutive efflux of citrate from roots.

    Science.gov (United States)

    Ryan, Peter R; Raman, Harsh; Gupta, Sanjay; Horst, Walter J; Delhaize, Emmanuel

    2009-01-01

    The first confirmed mechanism for aluminum (Al) resistance in plants is encoded by the wheat (Triticum aestivum) gene, TaALMT1, on chromosome 4DL. TaALMT1 controls the Al-activated efflux of malate from roots, and this mechanism is widespread among Al-resistant genotypes of diverse genetic origins. This study describes a second mechanism for Al resistance in wheat that relies on citrate efflux. Citrate efflux occurred constitutively from the roots of Brazilian cultivars Carazinho, Maringa, Toropi, and Trintecinco. Examination of two populations segregating for this trait showed that citrate efflux was controlled by a single locus. Whole-genome linkage mapping using an F(2) population derived from a cross between Carazinho (citrate efflux) and the cultivar EGA-Burke (no citrate efflux) identified a major locus on chromosome 4BL, Xce(c), which accounts for more than 50% of the phenotypic variation in citrate efflux. Mendelizing the quantitative variation in citrate efflux into qualitative data, the Xce(c) locus was mapped within 6.3 cM of the microsatellite marker Xgwm495 locus. This linkage was validated in a second population of F(2:3) families derived from a cross between Carazinho and the cultivar Egret (no citrate efflux). We show that expression of an expressed sequence tag, belonging to the multidrug and toxin efflux (MATE) gene family, correlates with the citrate efflux phenotype. This study provides genetic and physiological evidence that citrate efflux is a second mechanism for Al resistance in wheat.

  13. Spatial reorganization of the endoplasmic reticulum during mitosis relies on mitotic kinase cyclin A in the early Drosophila embryo.

    Science.gov (United States)

    Bergman, Zane J; Mclaurin, Justin D; Eritano, Anthony S; Johnson, Brittany M; Sims, Amanda Q; Riggs, Blake

    2015-01-01

    Mitotic cyclin-dependent kinase with their cyclin partners (cyclin:Cdks) are the master regulators of cell cycle progression responsible for regulating a host of activities during mitosis. Nuclear mitotic events, including chromosome condensation and segregation have been directly linked to Cdk activity. However, the regulation and timing of cytoplasmic mitotic events by cyclin:Cdks is poorly understood. In order to examine these mitotic cytoplasmic events, we looked at the dramatic changes in the endoplasmic reticulum (ER) during mitosis in the early Drosophila embryo. The dynamic changes of the ER can be arrested in an interphase state by inhibition of either DNA or protein synthesis. Here we show that this block can be alleviated by micro-injection of Cyclin A (CycA) in which defined mitotic ER clusters gathered at the spindle poles. Conversely, micro-injection of Cyclin B (CycB) did not affect spatial reorganization of the ER, suggesting CycA possesses the ability to initiate mitotic ER events in the cytoplasm. Additionally, RNAi-mediated simultaneous inhibition of all 3 mitotic cyclins (A, B and B3) blocked spatial reorganization of the ER. Our results suggest that mitotic ER reorganization events rely on CycA and that control and timing of nuclear and cytoplasmic events during mitosis may be defined by release of CycA from the nucleus as a consequence of breakdown of the nuclear envelope.

  14. Kinetochore function and chromosome segregation rely on critical residues in histones H3 and H4 in budding yeast.

    Science.gov (United States)

    Ng, Tessie M; Lenstra, Tineke L; Duggan, Nicole; Jiang, Shuangying; Ceto, Steven; Holstege, Frank C P; Dai, Junbiao; Boeke, Jef D; Biggins, Sue

    2013-11-01

    Accurate chromosome segregation requires that sister kinetochores biorient and attach to microtubules from opposite poles. Kinetochore biorientation relies on the underlying centromeric chromatin, which provides a platform to assemble the kinetochore and to recruit the regulatory factors that ensure the high fidelity of this process. To identify the centromeric chromatin determinants that contribute to chromosome segregation, we performed two complementary unbiased genetic screens using a library of budding yeast mutants in every residue of histone H3 and H4. In one screen, we identified mutants that lead to increased loss of a nonessential chromosome. In the second screen, we isolated mutants whose viability depends on a key regulator of biorientation, the Aurora B protein kinase. Nine mutants were common to both screens and exhibited kinetochore biorientation defects. Four of the mutants map near the unstructured nucleosome entry site, and their genetic interaction with reduced IPL1 can be suppressed by increasing the dosage of SGO1, a key regulator of biorientation. In addition, the composition of purified kinetochores was altered in six of the mutants. Together, this work identifies previously unknown histone residues involved in chromosome segregation and lays the foundation for future studies on the role of the underlying chromatin structure in chromosome segregation.

  15. Catalytic ozonation not relying on hydroxyl radical oxidation: A selective and competitive reaction process related to metal-carboxylate complexes

    KAUST Repository

    Zhang, Tao

    2014-01-01

    Catalytic ozonation following non-hydroxyl radical pathway is an important technique not only to degrade refractory carboxylic-containing organic compounds/matter but also to avoid catalyst deactivation caused by metal-carboxylate complexation. It is unknown whether this process is effective for all carboxylates or selective to special molecule structures. In this work, the selectivity was confirmed using O3/(CuO/CeO2) and six distinct ozone-resistant probe carboxylates (i.e., acetate, citrate, malonate, oxalate, pyruvate and succinate). Among these probe compounds, pyruvate, oxalate, and citrate were readily degraded following the rate order of oxalate>citrate>pyruvate, while the degradation of acetate, malonate, and succinate was not promoted. The selectivity was independent on carboxylate group number of the probe compounds and solution pH. Competitive degradation was observed for carboxylate mixtures following the preference order of citrate, oxalate, and finally pyruvate. The competitive degradation was ascribed to competitive adsorption on the catalyst surface. It was revealed that the catalytically degradable compounds formed bidentate chelating or bridging complexes with surface copper sites of the catalyst, i.e., the active sites. The catalytically undegradable carboxylates formed monodentate complexes with surface copper sites or just electrostatically adsorbed on the catalyst surface. The selectivity, relying on the structure of surface metal-carboxylate complex, should be considered in the design of catalytic ozonation process. © 2013 Elsevier B.V.

  16. Spatial Reorganization of the Endoplasmic Reticulum during Mitosis Relies on Mitotic Kinase Cyclin A in the Early Drosophila Embryo

    Science.gov (United States)

    Bergman, Zane J.; Mclaurin, Justin D.; Eritano, Anthony S.; Johnson, Brittany M.; Sims, Amanda Q.; Riggs, Blake

    2015-01-01

    Mitotic cyclin-dependent kinase with their cyclin partners (cyclin:Cdks) are the master regulators of cell cycle progression responsible for regulating a host of activities during mitosis. Nuclear mitotic events, including chromosome condensation and segregation have been directly linked to Cdk activity. However, the regulation and timing of cytoplasmic mitotic events by cyclin:Cdks is poorly understood. In order to examine these mitotic cytoplasmic events, we looked at the dramatic changes in the endoplasmic reticulum (ER) during mitosis in the early Drosophila embryo. The dynamic changes of the ER can be arrested in an interphase state by inhibition of either DNA or protein synthesis. Here we show that this block can be alleviated by micro-injection of Cyclin A (CycA) in which defined mitotic ER clusters gathered at the spindle poles. Conversely, micro-injection of Cyclin B (CycB) did not affect spatial reorganization of the ER, suggesting CycA possesses the ability to initiate mitotic ER events in the cytoplasm. Additionally, RNAi-mediated simultaneous inhibition of all 3 mitotic cyclins (A, B and B3) blocked spatial reorganization of the ER. Our results suggest that mitotic ER reorganization events rely on CycA and that control and timing of nuclear and cytoplasmic events during mitosis may be defined by release of CycA from the nucleus as a consequence of breakdown of the nuclear envelope. PMID:25689737

  17. The infectious intracellular lifestyle of Salmonella enterica relies on the adaptation to nutritional conditions within the Salmonella-containing vacuole.

    Science.gov (United States)

    Diacovich, Lautaro; Lorenzi, Lucía; Tomassetti, Mauro; Méresse, Stéphane; Gramajo, Hugo

    2016-12-09

    Salmonella enterica serovar Typhimurium (S. Typhimurium) is a Gram-negative pathogen that causes various host-specific diseases. During their life cycle, Salmonellae survive frequent exposures to a variety of environmental stresses, e.g. carbon-source starvation. The virulence of this pathogen relies on its ability to establish a replicative niche, named Salmonella-containing vacuole, inside host cells. However, the microenvironment of the SCV and the bacterial metabolic pathways required during infection are largely undefined. In this work we developed different biological probes whose expression is modulated by the environment and the physiological state of the bacterium. We constructed transcriptional reporters by fusing promoter regions to the gfpmut3a gene to monitor the expression profile of genes involved in glucose utilization and lipid catabolism. The induction of these probes by a specific metabolic change was first tested in vitro, and then during different conditions of infection in macrophages. We were able to determine that Entner-Doudoroff is the main metabolic pathway utilized by Salmonella during infection in mouse macrophages. Furthermore, we found sub-populations of bacteria expressing genes involved in pathways for the utilization of different sources of carbon. These populations are modified in presence of different metabolizable substrates, suggesting the coexistence of Salmonella with diverse metabolic states during the infection.

  18. Influence of computerized sounding out on spelling performance for children who do and do not rely on AAC.

    Science.gov (United States)

    McCarthy, Jillian H; Hogan, Tiffany P; Beukelman, David R; Schwarz, Ilsa E

    2015-05-01

    Spelling is an important skill for individuals who rely on augmentative and alternative communication (AAC). The purpose of this study was to investigate how computerized sounding out influenced spelling accuracy of pseudo-words. Computerized sounding out was defined as a word elongated, thus providing an opportunity for a child to hear all the sounds in the word at a slower rate. Seven children with cerebral palsy, four who use AAC and three who do not, participated in a single subject AB design. The results of the study indicated that the use of computerized sounding out increased the phonologic accuracy of the pseudo-words produced by participants. The study provides preliminary evidence for the use of computerized sounding out during spelling tasks for children with cerebral palsy who do and do not use AAC. Future directions and clinical implications are discussed. We investigated how computerized sounding out influenced spelling accuracy of pseudowords for children with complex communication needs who did and did not use augmentative and alternative communication (AAC). Results indicated that the use of computerized sounding out increased the phonologic accuracy of the pseudo-words by participants, suggesting that computerized sounding out might assist in more accurate spelling for children who use AAC. Future research is needed to determine how language and reading abilities influence the use of computerized sounding out with children who have a range of speech intelligibility abilities and do and do not use AAC.

  19. An Overview of the Characteristics and Function of Vitamin C in Various Tissues: Relying on its Antioxidant Function

    Directory of Open Access Journals (Sweden)

    Abolfazl Akbari

    2016-10-01

    Full Text Available Vitamin C (L-ascorbic acid or ascorbate is a biomolecule that participates in many biochemical processes. It is an essential nutrient for humans, however, in some species such as rodents and guinea pigs is synthesized. It has a variety of functions in the body that we might venture to say make it a very important antioxidant nature and pro-oxidant. L-ascorbic acidic a reduced form of vitamin C and dehydroascorbic acid (DHA is the oxidized form of ascorbate, both L-ascorbic acid and dihydroascorbic acid retain the vitamin C activity. Dehydro-ascorbate is reconverted to ascorbate in the cytosol by cytochrome b reductase and thioredoxin reductase in reactions involving NADH and NADPH, respectively. Ascorbate is transported into the cell via the sodium-dependent vitamin C transporters (SVCTs, which causes accumulation of ascorbate within cells against a concentration gradient. Dehydroascorbic acid, the oxidized form of ascorbate, is transported via glucose transporters family (GLUTs. The highest concentrations of ascorbate in the body are found in brain and adrenal gland. Vitamin C also acts as a co-factor in several enzyme reactions. This vitamin is an essential biochemical factor in the reproductive process. The pharmacophore of vitamin C is the ascorbate, ascorbate is an antioxidant.Ascorbate is a neuromodulator of glutamatergic and dopaminergic system and related behaviors. It also improves components of the immune system. Given the wide role of ascorbate, further investigation is necessary to evaluate the exact mechanism(s underlying these effects. In this review we will consider a short overview of the characteristics and function of vitamin C (relying on antioxidant function in various tissues.

  20. Regulation of Carotenoid Biosynthesis by Shade Relies on Specific Subsets of Antagonistic Transcription Factors and Cofactors1[OPEN

    Science.gov (United States)

    Bou-Torrent, Jordi; Toledo-Ortiz, Gabriela; Ortiz-Alcaide, Miriam; Cifuentes-Esquivel, Nicolas; Halliday, Karen J.; Martinez-García, Jaime F.; Rodriguez-Concepcion, Manuel

    2015-01-01

    Carotenoids are photosynthetic pigments essential for the protection against excess light. During deetiolation, their production is regulated by a dynamic repression-activation module formed by PHYTOCHROME-INTERACTING FACTOR1 (PIF1) and LONG HYPOCOTYL5 (HY5). These transcription factors directly and oppositely control the expression of the gene encoding PHYTOENE SYNTHASE (PSY), the first and main rate-determining enzyme of the carotenoid pathway. Antagonistic modules also regulate the responses of deetiolated plants to vegetation proximity and shade (i.e. to the perception of far-red light-enriched light filtered through or reflected from neighboring plants). These responses, aimed to adapt to eventual shading from plant competitors, include a reduced accumulation of carotenoids. Here, we show that PIF1 and related photolabile PIFs (but not photostable PIF7) promote the shade-triggered decrease in carotenoid accumulation. While HY5 does not appear to be required for this process, other known PIF antagonists were found to modulate the expression of the Arabidopsis (Arabidopsis thaliana) PSY gene and the biosynthesis of carotenoids early after exposure to shade. In particular, PHYTOCHROME-RAPIDLY REGULATED1, a transcriptional cofactor that prevents the binding of true transcription factors to their target promoters, was found to interact with PIF1 and hence directly induce PSY expression. By contrast, a change in the levels of the transcriptional cofactor LONG HYPOCOTYL IN FAR RED1, which also binds to PIF1 and other PIFs to regulate shade-related elongation responses, did not impact PSY expression or carotenoid accumulation. Our data suggest that the fine-regulation of carotenoid biosynthesis in response to shade relies on specific modules of antagonistic transcriptional factors and cofactors. PMID:26082398

  1. Summer kill rates and predation pattern in a wolf-moose system: can we rely on winter estimates?

    Science.gov (United States)

    Sand, Håkan; Wabakken, Petter; Zimmermann, Barbara; Johansson, Orjan; Pedersen, Hans C; Liberg, Olof

    2008-05-01

    So far the vast majority of studies on large carnivore predation, including kill rates and consumption, have been based on winter studies. Because large carnivores relying on ungulates as prey often show a preference for juveniles, kill rates may be both higher and more variable during the summer season than during the rest of the year leading to serious underestimates of the total annual predation rate. This study is the first to present detailed empirical data on kill rates and prey selection in a wolf-moose system during summer (June-September) as obtained by applying modern Global Positioning System-collar techniques on individual wolves (Canis lupus) in Scandinavia. Moose (Alces alces) was the dominant prey species both by number (74.4%) and biomass (95.6%); 89.9% of all moose killed were juveniles, representing 76.0% of the biomass consumed by wolves. Kill rate in terms of the kilogram biomass/kilogram wolf per day averaged 0.20 (range: 0.07-0.32) among wolf territories and was above, or well above, the daily minimum food requirements in most territories. The average number of days between moose kills across wolf territories and study periods was 1.71 days, but increased with time and size of growing moose calves during summer. Over the entire summer (June-September, 122 days), a group (from two to nine) of wolves killed a total of 66 (confidence interval 95%; 56-81) moose. Incorporation of body growth functions of moose calves and yearlings and wolf pups over the summer period showed that wolves adjusted their kill rate on moose, so the amount of biomass/kilogram wolf was relatively constant or increased. The kill rate was much higher (94-116%) than estimated from the winter period. As a consequence, projecting winter kill rates to obtain annual estimates of predation in similar predator-prey systems may result in a significant underestimation of the total number of prey killed.

  2. Marked Effect of Double Stapling Technique(DST) in Rectal Cancer(RC) Sphinctersparing Operation(SSO)%双吻合器技术在超低位直肠癌保肛术中的显效性分析(附108例报告)

    Institute of Scientific and Technical Information of China (English)

    童国俊; 张贵阳; 刘剑; 郑照正; 陈燕

    2013-01-01

    [目的]研究超低位直肠癌(rectal cancer,RC)使用双吻合器技术(double stapling technique,DST)在低位保肛手术(sphinctersparing operation,SSO)中的价值.[方法]对本院外科108例2005年1月到2010年1月保肛的RC手术患者按双吻合器吻合法(DST组)及其他吻合法(对照组)分为两组,分别对肿瘤距肛缘的距离、术后吻合口瘘例数、吻合口狭窄的例数、术后1月内死亡率、平均住院时间、平均住院费用、吻合口复发率7项指标进行比较分析以及对DST病例的排便功能进行分析.[结果]低位及超低位RC病例运用DST明显较多,差异有统计学意义(P<0.05);术后吻合口瘘、吻合口狭窄例数DST组低于对照组,差异有统计学意义(P<0.05);术后1月内死亡率、平均住院时间DST组明显较低,差异有统计学意义(P<0.05);平均住院费用、吻合口复发率无明显差异(P>005).DST组术后1年内排便功能基本恢复.[结论]在严格掌握低位SSO适应症的前提下,使用DST是安全有效的.%[Objective] Colorectal anastomosis in deep pelvis was very difficult to perform during Dixon operation. In this study we evaluated the role of double stapling technique in low colorectal anastomosis. [Method] By method of anastomosis(DST or not), 108 cases were divided into two groups which were admitted in our department of general surgery from Jan.2005 to Jan.2010 and performed by Dixon .We analyzed seven indexes which were the anal margin of carcinoma ,the number of anastomotic breakdown and stricture .mortality within one month ,mean time and cost of admission and the incidence of anastomotic recurrence. [Result] DST was more available in low and super low colorectal anastomosis ;There was of significant difference in the lndexes of the number of anastomotic breakdown and stricture .mortality within one month, meantime of admission between the two groups(P<0.05); while there was no significant difference in the indexes of mean

  3. Dažādu reliģiju duālā praktika un sinkrētisms. Katoļticība un Joruba

    OpenAIRE

    2014-01-01

    Darba tēma ir dažādu reliģiju duālā praktika un un sinkrētisms starp kristietību un jorubu. Darba mērķis ir izanalizēt sinkrētismu starp šīm divām reliģijām un to duālo praktiku. Pētījuma jautājumi ir sekojoši: kas ir jorubas reliģija un kādas ir tās īpašības un ticības, kā kristietība ir ietekmējusi jorubas reliģiju, kāds ir jorubas un kristietības sinkrētisma process un kā jorubas simboli tiek izmantoti mākslā. Darba gaitā tika secināts, ka kristietībai ir bijusi liela ietekme uz jorubu...

  4. Research Advances: Pacific Northwest National Laboratory Finds New Way to Detect Destructive Enzyme Activity--Hair Dye Relies on Nanotechnology--Ways to Increase Shelf Life of Milk

    Science.gov (United States)

    King, Angela G.

    2007-01-01

    Recent advances in various research fields are described. Scientists at the Pacific Northwest National Laboratory have found a new way to detect destructive enzyme activity, scientists in France have found that an ancient hair dye used by ancient people in Greece and Rome relied on nanotechnology and in the U.S. scientists are developing new…

  5. Research Advances: Pacific Northwest National Laboratory Finds New Way to Detect Destructive Enzyme Activity--Hair Dye Relies on Nanotechnology--Ways to Increase Shelf Life of Milk

    Science.gov (United States)

    King, Angela G.

    2007-01-01

    Recent advances in various research fields are described. Scientists at the Pacific Northwest National Laboratory have found a new way to detect destructive enzyme activity, scientists in France have found that an ancient hair dye used by ancient people in Greece and Rome relied on nanotechnology and in the U.S. scientists are developing new…

  6. Biological adhesion of the flatworm Macrostomum lignano relies on a duo-gland system and is mediated by a cell type-specific intermediate filament protein

    NARCIS (Netherlands)

    Lengerer, Birgit; Pjeta, Robert; Wunderer, Julia; Rodrigues, Marcelo; Arbore, Roberto; Schaerer, Lukas; Berezikov, Eugene; Hess, Michael W.; Pfaller, Kristian; Egger, Bernhard; Obwegeser, Sabrina; Salvenmoser, Willi; Ladurner, Peter

    2014-01-01

    Background: Free-living flatworms, in both marine and freshwater environments, are able to adhere to and release from a substrate several times within a second. This reversible adhesion relies on adhesive organs comprised of three cell types: an adhesive gland cell, a releasing gland cell, and an an

  7. Effect of GLUMA desensitizer on the retention of full metal crowns cemented with Rely X U200 self-adhesive cement

    Science.gov (United States)

    Lawaf, Shirin; Jalalian, Ezatallah; Roshan, Roshanak

    2016-01-01

    PURPOSE Considering the importance of retention in the success and long-term clinical service of fixed partial dentures (FPDs) as well as the existing controversy regarding the effect of GLUMA desensitizer on the retention of full metal crowns cemented with RelyX U200 self-adhesive cement, this study aimed to assess the effect of GLUMA desensitizer on the retention of full metal crowns cemented using RelyX U200. MATERIALS AND METHODS In this experimental study, 20 sound human premolars were prepared; a 0.5 mm chamfer finish line was prepared above the cementoenamel junction. The teeth were randomly assigned to two groups: a desensitizer group (n = 10, treated with GLUMA desensitizer) and a control (n = 10, no surface treatment). Full metal crowns were fabricated of base metal alloy and had a ring. All crowns were cemented with RelyX U200 and subjected to retention test by using a universal testing machine. The data were analyzed using SPSS version 20 and independent t-test. RESULTS The mean tensile bond strength was significantly higher in the GLUMA desensitizer group (230.63 ± 63.8 N) compared to the control group (164.45 ± 39.3 N) (P≤.012). CONCLUSION GLUMA desensitizer increases the tensile bond strength of RelyX U200 self-adhesive cement to dentin. PMID:27826391

  8. [Zeme, vara un reliģija viduslaikos un jaunajas laikos Baltijas jūras reģionā] / Marija Golubeva

    Index Scriptorium Estoniae

    Golubeva, Marija, 1973-

    2011-01-01

    Arvustus: Zeme, vara un reliģija viduslaikos un jaunajas laikos Baltijas jūras reģionā / Hrsg. von Andris Šnē. Acta Universitatis Latviensis ; 725. (Riga: Verlag Latvijas Universitātes Akadēmiskais apgāds, 2009)

  9. [Zeme, vara un reliģija viduslaikos un jaunajas laikos Baltijas jūras reģionā] / Marija Golubeva

    Index Scriptorium Estoniae

    Golubeva, Marija, 1973-

    2011-01-01

    Arvustus: Zeme, vara un reliģija viduslaikos un jaunajas laikos Baltijas jūras reģionā / Hrsg. von Andris Šnē. Acta Universitatis Latviensis ; 725. (Riga: Verlag Latvijas Universitātes Akadēmiskais apgāds, 2009)

  10. Lying relies on the truth

    NARCIS (Netherlands)

    Debey, E.; De Houwer, J.; Verschuere, B.

    2014-01-01

    Cognitive models of deception focus on the conflict-inducing nature of the truth activation during lying. Here we tested the counterintuitive hypothesis that the truth can also serve a functional role in the act of lying. More specifically, we examined whether the construction of a lie can involve a

  11. Lying relies on the truth

    NARCIS (Netherlands)

    Debey, E.; De Houwer, J.; Verschuere, B.

    2014-01-01

    Cognitive models of deception focus on the conflict-inducing nature of the truth activation during lying. Here we tested the counterintuitive hypothesis that the truth can also serve a functional role in the act of lying. More specifically, we examined whether the construction of a lie can involve a

  12. Cytotoxicity Comparison of Harvard Zinc Phosphate Cement Versus Panavia F2 and Rely X Plus Resin Cements on Rat L929-fibroblasts.

    Science.gov (United States)

    Mahasti, Sahabi; Sattari, Mandana; Romoozi, Elham; Akbar-Zadeh Baghban, Alireza

    2011-01-01

    Resin cements, regardless of their biocompatibility, have been widely used in restorative dentistry during the recent years. These cements contain hydroxy ethyl methacrylate (HEMA) molecules which are claimed to penetrate into dentinal tubules and may affect dental pulp. Since tooth preparation for metal ceramic restorations involves a large surface of the tooth, cytotoxicity of these cements would be more important in fixed prosthodontic treatments. The purpose of this study was to compare the cytotoxicity of two resin cements (Panavia F2 and Rely X Plus) versus zinc phosphate cement (Harvard) using rat L929-fibroblasts in vitro. In this experimental study, ninety hollow glass cylinders (internal diameter 5-mm, height 2-mm) were made and divided into three groups. Each group was filled with one of three experimental cements; Harvard Zinc Phosphate cement, Panavia F2 resin cement and Rely X Plus resin cement. L929- Fibroblast were passaged and subsequently cultured in 6-well plates of 5×10(5) cells each. The culture medium was RPMI_ 1640. All samples were incubated in CO2. Using enzyme-linked immune-sorbent assay (ELISA) and (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) (MTT) assay, the cytotoxicity of the cements was investigated at 1 hour, 24 hours and one week post exposure. Statistical analyses were performed via two-way ANOVA and honestly significant difference (HSD) Tukey tests. This study revealed significant differences between the three cements at the different time intervals. Harvard cement displayed the greatest cytotoxicity at all three intervals. After 1 hour Panavia F2 showed the next greatest cytotoxicity, but after 24-hours and oneweek intervals Rely X Plus showed the next greatest cytotoxicity. The results further showed that cytotoxicity decreased significantly in the Panavia F2 group with time (pcytotoxicity increased significantly in the Rely X Plus group with time (pcytotoxicity with time. Although this study has limitations

  13. Drugs with anti-oxidant properties can interfere with cell viability measurements by assays that rely on the reducing property of viable cells.

    Science.gov (United States)

    Shenoy, Niraj; Stenson, Mary; Lawson, Joshua; Abeykoon, Jithma; Patnaik, Mrinal; Wu, Xiaosheng; Witzig, Thomas

    2017-02-27

    Cell viability assays such as Cell Titer Blue and Alamar Blue rely on the reducing property of viable cells to reduce the reagent dye to a product which gives a fluorescent signal. The current manufacture-recommended protocols do not take into account the possibility of the reagent substrate being reduced directly to the fluorescent product by drugs with an anti-oxidant property. After suspecting spurious results while determining the cytotoxic potential of a drug of interest (DOI) with known anti-oxidant property against a renal cell cancer (RCC) cell line, we aimed to establish that drugs with anti-oxidant property can indeed cause false-negative results with the current protocols of these assays by direct reduction of the reagent substrate. We also aimed to counter the same with a simple modification added to the protocol. Through our experiments, we conclusively demonstrate that drugs with anti-oxidant properties can indeed interfere with cell viability measurements by assays that rely on the reducing property of viable cells. A simple modification in the protocol, as elaborated in the manuscript, can prevent spurious results with these otherwise convenient assays.Laboratory Investigation advance online publication, 27 February 2017; doi:10.1038/labinvest.2017.18.

  14. A series of meta-analytic tests of the depletion effect: Self-control does not seem to rely on a limited resource.

    Science.gov (United States)

    Carter, Evan C; Kofler, Lilly M; Forster, Daniel E; McCullough, Michael E

    2015-08-01

    Failures of self-control are thought to underlie various important behaviors (e.g., addiction, violence, obesity, poor academic achievement). The modern conceptualization of self-control failure has been heavily influenced by the idea that self-control functions as if it relied upon a limited physiological or cognitive resource. This view of self-control has inspired hundreds of experiments designed to test the prediction that acts of self-control are more likely to fail when they follow previous acts of self-control (the depletion effect). Here, we evaluated the empirical evidence for this effect with a series of focused, meta-analytic tests that address the limitations in prior appraisals of the evidence. We find very little evidence that the depletion effect is a real phenomenon, at least when assessed with the methods most frequently used in the laboratory. Our results strongly challenge the idea that self-control functions as if it relies on a limited psychological or physical resource. (c) 2015 APA, all rights reserved).

  15. High school students rely on phone and analysis of the state of personality%高中生手机依赖与人格状况分析

    Institute of Scientific and Technical Information of China (English)

    吴玉芳

    2015-01-01

    随着移动互联网的迅猛发展,青少年网民的急剧增加,对手机依赖也趋向年轻化,为了了解高中生手机依赖的现状及其与焦虑、人格特征的关系,随机选取福州某中学168名学生,采用问卷调查的形式,运用列联表相关分析等统计方法分析高中生手机依赖与焦虑、人格特征的关系。结果表明,高中生手机依赖与焦虑无相关关系,手机依赖在人格特质三个维度(内外向、精神质以及神经质)上均存在显著差异,所对应的的概率值分别为0.004<0.05、0.023<0.05、0.04<0.05.%With the rapid development of mobile Internet, the rapid increase of teenagers, the dependency on mobile phones tend to be younger, in order to understand the present situation of the mobile phone rely on high school students and its relationship with anxiety, personality characteristics, at the middle school, 168 students randomly selected from Fuzhou, in the form of questionnaire survey, the use of contingency table analysis and so on related statistical methods to analyze mobile phone rely on high school students and anxiety, the relationship between personality traits. Results show that high school students phone no correlation with anxiety, rely on mobile phone on personality traits three dimensions (inside and outside, psychoticism and neuroticism) there were significant differences, the corresponding probability value was 0.004 < 0.05, 0.023 < 0.05, 0.04 < 0.05.

  16. 依托社区居家养老--老年社区设计探索%Relying on Community Home Care--Elderly Community Design Exploration

    Institute of Scientific and Technical Information of China (English)

    杨媛婷

    2013-01-01

      人口老龄化是未来发展的一个重要趋势。如何解决老龄人口的养老问题成为我们所要面临的重大课题。依托社区居家养老是符合我国国情的老年人主要养老模式。那么如何创造适合老年人需求的居住社区,已经逐渐成为社区设计所急待解决的问题。本文通过对老年人的生理特征、心理特征、行为特点等方面的调查与分析,对老年社区居住空间、老年社区环境的空间功能及细部设计原则进行探索研究,提出适合老年人居家养老的社区设计方法,使老年人真正做到依托社区居家养老。%The aging of the population is an important trend of development in the future. How to solve the pension proble-m of aging population has become a major issue we faced. Re-lying on the community home endowment is the pension mode for china. So how to create suitable for the needs of the elderly living community, has become the urgent problems in commu-nity design. Though the investigation and analysis of old peop-le’s physiological characteristics, the psychological characteri-stics, behavioral characteristics, this paper studies the spatial f-unction and detail design principle of environment of the old c-ommunity living space, the old community, the community de-sign suitable for the elderly pension law, made old people truly rely on the community home endowment.

  17. No gender differences in the frequencies of HLA-DRB3/B4/B5 heterozygotes in newborns and adults in Koreans.

    Science.gov (United States)

    Song, Eun Young; Roh, Eun Youn; Shin, Sue; Yoon, Jong Hyun; Park, Myoung Hee

    2012-01-01

    HLA class II haplotypes often contain a second expressed HLA-DRB locus tightly linked to the classical HLA-DRB1 locus on the haplotype, which can be either HLA-DRB3, -DRB4 or -DRB5. These encode the HLA-DR51, -DR52 or -DR53 supertypic specificities and mark the ancestral lineages. HLA-DRB3/B4/B5 heterozygote excess in Welsh male newborns has been reported, suggesting a possibility of male-specific major histocompatibility complex (MHC)-mediated prenatal selection. However, it has not been confirmed in newborns of other ethnic groups or in adult populations. We analyzed the HLA-DRB1 and HLA-DRB3/B4/B5 genes in Korean newborns and healthy adults to examine whether MHC-mediated prenatal or postnatal selection exists. A total of 1,038 newborns (cord blood registry, 516 males and 522 females) and 2,082 healthy adults (hematopoietic stem cell donor registry, 1,111 males and 971 females) were HLA typed. HLA-DRB1/B3/B4/B5 DNA typing was performed using Dynal RELI HLA-DRB SSO Kit (Dyanl Biotech, Wirral, U.K.). Genotype frequencies and homozygosity and heterozygosity rates for DRB3/B4/B5 supertypic loci were compared between males and females in newborns and adults. There were no significant differences in the HLA-DRB3/B4/B5 homozygosity and heterozygosity rates between males and females in both newborns and adults. In the comparison between newborns and adults, homozygosity rate was significantly higher in newborn females than in adult females (31.0% vs 25.0%, p=0.01). Whether there is an age-related change from newborns toward adults has not been well studied in other populations, and further studies are warranted. In conclusion, male-specific heterozygosity excess reported in Welsh newborns has not been observed in Korean population, and there might be some ethnic differences in the gender-specific prenatal selection events.

  18. Moralized Rationality: Relying on Logic and Evidence in the Formation and Evaluation of Belief Can Be Seen as a Moral Issue.

    Science.gov (United States)

    Ståhl, Tomas; Zaal, Maarten P; Skitka, Linda J

    2016-01-01

    In the present article we demonstrate stable individual differences in the extent to which a reliance on logic and evidence in the formation and evaluation of beliefs is perceived as a moral virtue, and a reliance on less rational processes is perceived as a vice. We refer to this individual difference variable as moralized rationality. Eight studies are reported in which an instrument to measure individual differences in moralized rationality is validated. Results show that the Moralized Rationality Scale (MRS) is internally consistent, and captures something distinct from the personal importance people attach to being rational (Studies 1-3). Furthermore, the MRS has high test-retest reliability (Study 4), is conceptually distinct from frequently used measures of individual differences in moral values, and it is negatively related to common beliefs that are not supported by scientific evidence (Study 5). We further demonstrate that the MRS predicts morally laden reactions, such as a desire for punishment, of people who rely on irrational (vs. rational) ways of forming and evaluating beliefs (Studies 6 and 7). Finally, we show that the MRS uniquely predicts motivation to contribute to a charity that works to prevent the spread of irrational beliefs (Study 8). We conclude that (1) there are stable individual differences in the extent to which people moralize a reliance on rationality in the formation and evaluation of beliefs, (2) that these individual differences do not reduce to the personal importance attached to rationality, and (3) that individual differences in moralized rationality have important motivational and interpersonal consequences.

  19. Automatic and intentional number processing both rely on intact right parietal cortex: A combined fMRI and neuronavigated TMS study.

    Directory of Open Access Journals (Sweden)

    Roi eCohen Kadosh

    2012-02-01

    Full Text Available Practice and training usually lead to performance increase in a given task. In addition, a shift from intentional towards more automatic processing mechanisms is often observed. It is currently debated whether automatic and intentional processing is subserved by the same or by different mechanism(s, and whether the same or different regions in the brain are recruited. Previous correlational evidence provided by behavioural, neuroimaging, modelling, and neuropsychological studies addressing this question yielded conflicting results. Here we used transcranial magnetic stimulation (TMS to compare the causal influence of disrupting either left or right parietal cortex during automatic and intentional numerical processing, as reflected by the size congruity effect and the numerical distance effect, respectively. We found a functional hemispheric asymmetry within parietal cortex with only the TMS-induced right parietal disruption impairing both automatic and intentional numerical processing. In contrast, disrupting the left parietal lobe with TMS, or applying sham stimulation, did not affect performance during automatic or intentional numerical processing. The current results provide causal evidence for the functional relevance of right, but not left, parietal cortex for intentional and automatic numerical processing, implying that at least within the parietal cortices, automatic and intentional numerical processing rely on the same underlying hemispheric lateralization.

  20. Females and males rely on different cortical regions in Raven's Matrices reasoning capacity: evidence from a voxel-based morphometry study.

    Directory of Open Access Journals (Sweden)

    Wenjing Yang

    Full Text Available Raven's Matrices test (RMT is a non-verbal test designed to assess individuals' ability to reason and solve new problems without relying extensively on declarative knowledge derived from schooling or previous experience. Despite a large number of behavioral studies that demonstrated gender differences in Raven's Matrices reasoning ability, no neural evidence supported this difference. In this study, voxel-based morphometry (VBM was used in an attempt to uncover the gender-specific neural basis of Raven's Matrices reasoning ability as measured by the combined Raven's Matrices test (CRT in 370 healthy young adults. The behavioral results showed no difference between males and females. However, the VBM results showed that the relationship between reasoning ability and regional gray matter volume (rGMV differed between sexes. The association between CRT scores and rGMV in the dorsolateral prefrontal cortex (associated with visuospatial ability was significantly greater in males than in females, whereas the reverse was true for the inferior frontal cortex (relating to verbal reasoning ability and the medial frontal cortex (engaged in information binding where the association was greater in females. These findings suggest that males and females use differently structured brains in different ways to achieve similar levels of overall Raven's Matrices reasoning ability.

  1. When Excessive Perturbation Goes Wrong and Why IPUMS-International Relies Instead on Sampling, Suppression, Swapping, and Other Minimally Harmful Methods to Protect Privacy of Census Microdata

    Science.gov (United States)

    Cleveland, Lara; McCaa, Robert; Ruggles, Steven; Sobek, Matthew

    2016-01-01

    IPUMS-International disseminates population census microdata at no cost for 69 countries. Currently, a series of 212 samples totaling almost a half billion person records are available to researchers. Registration is required for researchers to gain access to the microdata. Statistics from Google Analytics show that IPUMS-International’s lengthy, probing registration form is an effective deterrent for unqualified applicants. To protect data privacy, we rely principally on sampling, suppression of geographic detail, swapping of records across geographic boundaries, and other minimally harmful methods such as top and bottom coding. We do not use excessively perturbative methods. A recent case of perturbation gone wrong— the household samples of the 2000 census of the USA (PUMS), the 2003–2006 American Community Survey, and the 2004–2009 Current Population Survey—, an empirical study of the impact of perturbation on the usability of UK census microdata—the Individual SARs of the 1991 census of the UK—, and a mathematical demonstration in a timely compendium of statistical confidentiality practices confirm the wisdom of IPUMS microdata management protocols and statistical disclosure controls.

  2. Cytosolic NADPH homeostasis in glucose-starved procyclic Trypanosoma brucei relies on malic enzyme and the pentose phosphate pathway fed by gluconeogenic flux.

    Science.gov (United States)

    Allmann, Stefan; Morand, Pauline; Ebikeme, Charles; Gales, Lara; Biran, Marc; Hubert, Jane; Brennand, Ana; Mazet, Muriel; Franconi, Jean-Michel; Michels, Paul A M; Portais, Jean-Charles; Boshart, Michael; Bringaud, Frédéric

    2013-06-21

    All living organisms depend on NADPH production to feed essential biosyntheses and for oxidative stress defense. Protozoan parasites such as the sleeping sickness pathogen Trypanosoma brucei adapt to different host environments, carbon sources, and oxidative stresses during their infectious life cycle. The procyclic stage develops in the midgut of the tsetse insect vector, where they rely on proline as carbon source, although they prefer glucose when grown in rich media. Here, we investigate the flexible and carbon source-dependent use of NADPH synthesis pathways in the cytosol of the procyclic stage. The T. brucei genome encodes two cytosolic NADPH-producing pathways, the pentose phosphate pathway (PPP) and the NADP-dependent malic enzyme (MEc). Reverse genetic blocking of those pathways and a specific inhibitor (dehydroepiandrosterone) of glucose-6-phosphate dehydrogenase together established redundancy with respect to H2O2 stress management and parasite growth. Blocking both pathways resulted in ∼10-fold increase of susceptibility to H2O2 stress and cell death. Unexpectedly, the same pathway redundancy was observed in glucose-rich and glucose-depleted conditions, suggesting that gluconeogenesis can feed the PPP to provide NADPH. This was confirmed by (i) a lethal phenotype of RNAi-mediated depletion of glucose-6-phosphate isomerase (PGI) in the glucose-depleted Δmec/Δmec null background, (ii) an ∼10-fold increase of susceptibility to H2O2 stress observed for the Δmec/Δmec/(RNAi)PGI double mutant when compared with the single mutants, and (iii) the (13)C enrichment of glycolytic and PPP intermediates from cells incubated with [U-(13)C]proline, in the absence of glucose. Gluconeogenesis-supported NADPH supply may also be important for nucleotide and glycoconjugate syntheses in the insect host.

  3. How and When Do Insects Rely on Endogenous Protein and Lipid Resources during Lethal Bouts of Starvation? A New Application for 13C-Breath testing.

    Directory of Open Access Journals (Sweden)

    Marshall D McCue

    Full Text Available Most of our understanding about the physiology of fasting and starvation comes from studies of vertebrates; however, for ethical reasons, studies that monitor vertebrates through the lethal endpoint are scant. Insects are convenient models to characterize the comparative strategies used to cope with starvation because they have diverse life histories and have evolved under the omnipresent challenge of food limitation. Moreover, we can study the physiology of starvation through its natural endpoint. In this study we raised populations of five species of insects (adult grasshoppers, crickets, cockroaches, and larval beetles and moths on diets labeled with either 13C-palmitic acid or 13C-leucine to isotopically enrich the lipids or the proteins in their bodies, respectively. The insects were allowed to become postabsorptive and then starved. We periodically measured the δ13C of the exhaled breath to characterize how each species adjusted their reliance on endogenous lipids and proteins as energy sources. We found that starving insects employ a wide range of strategies for regulating lipid and protein oxidation. All of the insects except for the beetle larvae were capable of sharply reducing reliance on protein oxidation; however, this protein sparing strategy was usually unsustainable during the entire starvation period. All insects increased their reliance on lipid oxidation, but while some species (grasshoppers, cockroaches, and beetle larvae were still relying extensively on lipids at the time of death, other species (crickets and moth larvae allowed rates of lipid oxidation to return to prestarvation levels. Although lipids and proteins are critical metabolic fuels for both vertebrates and insects, insects apparently exhibit a much wider range of strategies for rationing these limited resources during starvation.

  4. Can grass phytoliths and indices be relied on during vegetation and climate interpretations in the eastern Himalayas? Studies from Darjeeling and Arunachal Pradesh, India

    Science.gov (United States)

    Biswas, Oindrila; Ghosh, Ruby; Paruya, Dipak Kumar; Mukherjee, Biswajit; Thapa, Kishore Kumar; Bera, Subir

    2016-02-01

    While documenting the vegetation response to climatic changes in mountains, the use of grass phytolith data relies on the ability of phytolith assemblages or indices to differentiate the elevationally stratified vegetation zones. To infer the potential and limitations of grass phytolith assemblages and indices to reconstruct vegetation vis-à-vis climate in the Himalayan mountain regions, we analyzed phytolith assemblages from 66 dominant grasses and 153 surface soils from four different forest types along the c. 130-4000 m a.s.l. elevation gradients in the Darjeeling and Arunachal Himalayas. Grass short cell phytolith assemblages from modern grasses show significant variability with rising elevation. To test the reliability of the above observation, phytoliths from the soil samples were subjected to linear discriminant analysis (DA). DA classified 85.3% and 92.3% of the sites to their correct forest zones in the Darjeeling and Arunachal Himalayas respectively. Relative abundance of bilobate, cross, short saddle, plateau saddle, rondel and trapeziform types allow discrimination of the phytolith assemblage along the elevation gradient. Canonical correspondence analysis (CCA) on the soil phytolith data further revealed their relationships with the climatic variables. Temperature and evapotranspiration were found to be the most influential for differential distribution of grass phytolith assemblages with rising elevation in the eastern Himalayas. We also tested the reliability of phytolith indices (Ic, Iph and Fs) for tracing the dominance of different grass subfamilies in the eastern Himalayas. Ic proved to be most reliable in discriminating C3/C4 grass along the elevation gradient while Iph and Fs proved to be less reliable. We observed that in the monsoon dominated eastern Himalayas, a little adjustment in Ic index may enhance the accuracy of interpretations. In future studies more precise identification of phytolith sub-types from additional sites in the eastern

  5. Stepped MS(All) Relied Transition (SMART): An approach to rapidly determine optimal multiple reaction monitoring mass spectrometry parameters for small molecules.

    Science.gov (United States)

    Ye, Hui; Zhu, Lin; Wang, Lin; Liu, Huiying; Zhang, Jun; Wu, Mengqiu; Wang, Guangji; Hao, Haiping

    2016-02-11

    Multiple reaction monitoring (MRM) is a universal approach for quantitative analysis because of its high specificity and sensitivity. Nevertheless, optimization of MRM parameters remains as a time and labor-intensive task particularly in multiplexed quantitative analysis of small molecules in complex mixtures. In this study, we have developed an approach named Stepped MS(All) Relied Transition (SMART) to predict the optimal MRM parameters of small molecules. SMART requires firstly a rapid and high-throughput analysis of samples using a Stepped MS(All) technique (sMS(All)) on a Q-TOF, which consists of serial MS(All) events acquired from low CE to gradually stepped-up CE values in a cycle. The optimal CE values can then be determined by comparing the extracted ion chromatograms for the ion pairs of interest among serial scans. The SMART-predicted parameters were found to agree well with the parameters optimized on a triple quadrupole from the same vendor using a mixture of standards. The parameters optimized on a triple quadrupole from a different vendor was also employed for comparison, and found to be linearly correlated with the SMART-predicted parameters, suggesting the potential applications of the SMART approach among different instrumental platforms. This approach was further validated by applying to simultaneous quantification of 31 herbal components in the plasma of rats treated with a herbal prescription. Because the sMS(All) acquisition can be accomplished in a single run for multiple components independent of standards, the SMART approach are expected to find its wide application in the multiplexed quantitative analysis of complex mixtures.

  6. Moralized Rationality: Relying on Logic and Evidence in the Formation and Evaluation of Belief Can Be Seen as a Moral Issue

    Science.gov (United States)

    Skitka, Linda J.

    2016-01-01

    In the present article we demonstrate stable individual differences in the extent to which a reliance on logic and evidence in the formation and evaluation of beliefs is perceived as a moral virtue, and a reliance on less rational processes is perceived as a vice. We refer to this individual difference variable as moralized rationality. Eight studies are reported in which an instrument to measure individual differences in moralized rationality is validated. Results show that the Moralized Rationality Scale (MRS) is internally consistent, and captures something distinct from the personal importance people attach to being rational (Studies 1–3). Furthermore, the MRS has high test-retest reliability (Study 4), is conceptually distinct from frequently used measures of individual differences in moral values, and it is negatively related to common beliefs that are not supported by scientific evidence (Study 5). We further demonstrate that the MRS predicts morally laden reactions, such as a desire for punishment, of people who rely on irrational (vs. rational) ways of forming and evaluating beliefs (Studies 6 and 7). Finally, we show that the MRS uniquely predicts motivation to contribute to a charity that works to prevent the spread of irrational beliefs (Study 8). We conclude that (1) there are stable individual differences in the extent to which people moralize a reliance on rationality in the formation and evaluation of beliefs, (2) that these individual differences do not reduce to the personal importance attached to rationality, and (3) that individual differences in moralized rationality have important motivational and interpersonal consequences. PMID:27851777

  7. How and When Do Insects Rely on Endogenous Protein and Lipid Resources during Lethal Bouts of Starvation? A New Application for 13C-Breath testing.

    Science.gov (United States)

    McCue, Marshall D; Guzman, R Marena; Passement, Celeste A; Davidowitz, Goggy

    2015-01-01

    Most of our understanding about the physiology of fasting and starvation comes from studies of vertebrates; however, for ethical reasons, studies that monitor vertebrates through the lethal endpoint are scant. Insects are convenient models to characterize the comparative strategies used to cope with starvation because they have diverse life histories and have evolved under the omnipresent challenge of food limitation. Moreover, we can study the physiology of starvation through its natural endpoint. In this study we raised populations of five species of insects (adult grasshoppers, crickets, cockroaches, and larval beetles and moths) on diets labeled with either 13C-palmitic acid or 13C-leucine to isotopically enrich the lipids or the proteins in their bodies, respectively. The insects were allowed to become postabsorptive and then starved. We periodically measured the δ13C of the exhaled breath to characterize how each species adjusted their reliance on endogenous lipids and proteins as energy sources. We found that starving insects employ a wide range of strategies for regulating lipid and protein oxidation. All of the insects except for the beetle larvae were capable of sharply reducing reliance on protein oxidation; however, this protein sparing strategy was usually unsustainable during the entire starvation period. All insects increased their reliance on lipid oxidation, but while some species (grasshoppers, cockroaches, and beetle larvae) were still relying extensively on lipids at the time of death, other species (crickets and moth larvae) allowed rates of lipid oxidation to return to prestarvation levels. Although lipids and proteins are critical metabolic fuels for both vertebrates and insects, insects apparently exhibit a much wider range of strategies for rationing these limited resources during starvation.

  8. An optimized single chain TCR scaffold relying on the assembly with the native CD3-complex prevents residual mispairing with endogenous TCRs in human T-cells.

    Science.gov (United States)

    Knies, Diana; Klobuch, Sebastian; Xue, Shao-An; Birtel, Matthias; Echchannaoui, Hakim; Yildiz, Oezlem; Omokoko, Tana; Guillaume, Philippe; Romero, Pedro; Stauss, Hans; Sahin, Ugur; Herr, Wolfgang; Theobald, Matthias; Thomas, Simone; Voss, Ralf-Holger

    2016-04-19

    Immunotherapy of cancer envisions the adoptive transfer of T-cells genetically engineered with tumor-specific heterodimeric α/β T-cell receptors (TCRα/β). However, potential mispairing of introduced TCRα/β-chains with endogenous β/α-ones may evoke unpredictable autoimmune reactivities. A novel single chain (sc)TCR format relies on the fusion of the Vα-Linker-Vβ-fragment to the TCR Cβ-domain and coexpression of the TCR Cα-domain capable of recruiting the natural CD3-complex for full and hence, native T-cell signaling. Here, we tested whether such a gp100(280-288)- or p53(264-272) tumor antigen-specific scTCR is still prone to mispairing with TCRα. In a human Jurkat-76 T-cell line lacking endogenous TCRs, surface expression and function of a scTCR could be reconstituted by any cointroduced TCRα-chain indicating mispairing to take place on a molecular basis. In contrast, transduction into human TCRα/β-positive T-cells revealed that mispairing is largely reduced. Competition experiments in Jurkat-76 confirmed the preference of dcTCR to selfpair and to spare scTCR. This also allowed for the generation of dc/scTCR-modified cytomegalovirus/tumor antigen-bispecific T-cells to augment T-cell activation in CMV-infected tumor patients. Residual mispairing was prevented by strenghtening the Vα-Li-Vβ-fragment through the design of a novel disulfide bond between a Vα- and a linker-resident residue close to Vβ. Multimer-stainings, and cytotoxicity-, IFNγ-secretion-, and CFSE-proliferation-assays, the latter towards dendritic cells endogenously processing RNA-electroporated gp100 antigen proved the absence of hybrid scTCR/TCRα-formation without impairing avidity of scTCR/Cα in T-cells. Moreover, a fragile cytomegalovirus pp65(495-503)-specific scTCR modified this way acquired enhanced cytotoxicity. Thus, optimized scTCR/Cα inhibits residual TCR mispairing to accomplish safe adoptive immunotherapy for bulk endogenous TCRα/β-positive T-cells.

  9. [Analysis of EDS fingerprint spectra of mineral drug montmorillonite powder relying on dual index grade sequence individualized pattern recognition method and their quickly quality evaluation].

    Science.gov (United States)

    Zou, Hua-Bin; Ayiguzaili; Ablimiti; Zhai, Hong

    2013-12-01

    EDS(energy dispersive spectrometer) element fingerprint spectra is able to quickly measure the kinds and the contents of elements in any mineral drug. In dual index grade sequence individualized pattern recognition method, common (quantity) index and variation (quantity) index ratios of any two samples' fingerprint spectra are calculated, and the individualized dual index sequence of each sample is constructed relying on its own EDS fingerprint spectra as the reference. Then the mean common (quantity) index ratio P and the standard deviation S of all samples in each sample's individualized dual index sequence are computed. On this basis, for each sample, its own similarity scale function P> or =P+xS is built up. By this function, the optimum x suitable for optimized classification/cluster of all samples is determined, and the individualized characteristics sequence of one sample, to which samples in the individualized characteristics sequence are significantly similar, is decided also. Finally, depending on these individualized characteristics sequences, the optimized classification/cluster of all samples can be carried out perfectly without any prior knowledge related to them. This method is not only suitable for the quantitative analysis on fingerprint spectra being of only common peaks, but also fits for that being of both common and variant peaks. In this study, the EDS element fingerprint spectra of seven mineral drug montmorillonite powder samples from different companies were detected. Then common (quantity) index and variant (quantity) index ratios of peak area (or contents of majorly active element Fe, Al, Ca, Mg, Si) among different EDS fingerprint spectra were obtained. In the similarity scale function P> or =P+xS, when x= 0. 5, these seven mineral drug montmorillonite powder samples could be quickly identified with high resolution, be classified into two groups, and their quality could be evaluated precisely. In general EDS element fingerprint spectra

  10. Optimization of covalent immobilization of Trichoderma reesei cellulase onto modified ReliZyme HA403 and Sepabeads EC-EP supports for cellulose hydrolysis, in buffer and ionic liquids/buffer media.

    Science.gov (United States)

    Bilgin, Ramazan; Yalcin, M Serkan; Yildirim, Deniz

    2016-08-01

    The covalent immobilization of Trichoderma reesei cellulase onto modified ReliZyme HA403 and Sepabeads EC-EP supports were carried out. The optimal immobilization conditions were determined using response surface methodology. The hydrolysis of cellulose using the free and immobilized cellulase preparations in ionic liquids (IL) using cosolvents was investigated. The hydrolytic activities in buffer medium containing 25% (v/v) of 1-butyl-3-methylimidazolium hexafluorophosphate were around 2.6-, 1.6-, and 5.5-fold higher than the activities in buffer medium. The retained initial activities were 32% and 57%, respectively for cellulase preparations immobilized onto Sepabeads EC-EP support and onto modified ReliZyme HA403 support after 5 reuses.

  11. Summary of the 2010 AHPBA/SSO/SSAT Consensus Conference on HCC

    Directory of Open Access Journals (Sweden)

    Gitonga Munene

    2011-01-01

    Full Text Available Under the auspices of the American Hepato-Pancreato-Biliary Association, an expert consensus conference was convened in January 2010 on the multidisciplinary management of hepatocellular carcinoma. The goals of the conference were to address knowledge gaps in the optimal preparation of patients with HCC for operative therapy, best methods to control HCC while awaiting liver transplantation, and developing a multidisciplinary approach to these patients with implementation of novel systemic therapies.

  12. AMBIGUITY OF LOCI DURING HLA-TYPING ON SSO TECHNOLOGY AND ATTEMPT TO RESOLVE THEM

    Directory of Open Access Journals (Sweden)

    M. A. Loginova

    2010-01-01

    Full Text Available Sequence specific oligonucleotides typing was used to identify human leukocyte antigen (HLA-A, B, DRB1 alleles from 705 recruited volunteers with Volga Federal District for unrelated hematopoietic stem cell registry and 155 of their number at locus HLA-C. 48 samples cannot be entered into the database because of ambiguities in the identification of allelic loci on HLA-class I – HLA-A, HLA-B. To resolution of ambiguity use reagents kits AlleleSEQR HLA Sequencing, which allowed to reveal the ambiguity of the locus HLA-A, 44% of cases, the loci HLA-B and HLA-C – 40% of cases. Application software HARPs Finder showed the possibility of resolution of all identified allelic ambiguities (with the exception of types of ambiguity – A*03/A*32 or A*74:13/A*32:04 and A*01/A*11 or A*36:04/A*11 with the addition of basic kits AlleleSEQR HLA Sequencing reagents kit Al- leleSEQR HARPs. 

  13. Twee reliëfs herenigd

    NARCIS (Netherlands)

    van Haarlem, W.

    2016-01-01

    Recently, the Museum acquired a new relief from the tomb of Saiempetref (reign of Seti I), belonging to a piece which was already in the collection. It has three registers; the upper one is showing the Solar Boat, the middle one the deceased and his wife offering to Osiris, and the third register

  14. Should We Rely on Intelligence Cycle?

    OpenAIRE

    2015-01-01

    Intelligence cycle is a systematic process which is usually applied in order to obtain intelligence from raw data. Today the world has been experiencing dazzling changes on many fields as well as technology. With the help of the new emerging technologies the data that have to be handled for intelligence is much more than ever. In addition to new technological contributions, there are discussions about intelligence cycle whether it’s being out-dated and old fashioned. Intelligence cycle was ma...

  15. Electricity Saving Relies on Mechanism Innovation

    Institute of Scientific and Technical Information of China (English)

    Ye Chun; Ye Qing

    2007-01-01

    @@ Energy saving is a vital important issue concerning sustainable development of social economy including national economic security, international competitiveness, resources conservation and environmental protection. Energy saving has now become one of the most important national strategy of China in the 21st Century. The Author believes, mechanism innovation to realize new type of management system, that is, to stipulate quota and efficiency standards of power utilization,to set up incentive and restrictive mechanism taking electricity price as a leverage so as to intensify the leading role of electricity price on power demand that electricity saving needs.

  16. 水产食品特定腐败菌与货架期的预测和延长%Specific spoilage organisms from aquatic product and prediction & prolongation of the shelf life

    Institute of Scientific and Technical Information of China (English)

    杨宪时; 许钟; 肖琳琳

    2004-01-01

    Fresh fish and lightly preserved fish products are welcome by the global market, however, they are also among the most perishable food products. The research on specific spoilage organisms (SSO) reveals the spoilage process of aquatic product. This paper reviews the current knowledge (past ten years) on SSO of fresh fish and lightly preserved fish products with particular emphasis on characteristics of SSO and how to apply this concept to determine, predict and extend the shelf life of aquatic product. During storage, the microflora changes owing to different abilities of the microorganisms to tolerate the preservation conditions. SSO is defined as special microorganisms which can increase rapidly during preservation and has the ability to produce off- odours and off- flavours associated with spoilage, and spoilage metabolites. Identification of an SSO relies on comparison of the sensory and chemical characteristics of spoiled product with those of isolates from the spoilage microflora. Generally, the SSO of fresh fish may be a single species or genus, but the ones of lightly preserved fish products will be more complex. One exciting area for use of SSO aims to obtain quantitative knowledge about probable behavior of SSO and their function during the progression of spoilage. Thus mathematical models on the growth of SSO are established to evaluate the quality lost degree of product, which provide a sound information for the rational development of devices to monitor loss of products shelf life. Models for the growth of Pseudomonas spp, S. putrefaciens, P. phosphoreum have been established, and validated for shelf life prediction of seafood successfully. Another application field of SSO intends to develop the techniques to prolong the shelf life of food products by inhibiting SSO targetedly. Targeted inhibition of spoilage bacteria during preservation reduces their growth and results in a significant extension of shelf life in despite of the activity of non

  17. The RELi{sub x}Sn{sub 2} (RE=La–Nd, Sm, and Gd; 0≤x<1) series revisited. Synthesis, crystal chemistry, and magnetic susceptibilities

    Energy Technology Data Exchange (ETDEWEB)

    Makongo, Julien P.A.; Suen, Nian-Tzu; Guo, Shengping [Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716 (United States); Saha, Shanta; Greene, Richard; Paglione, Johnpierre [Department of Physics, University of Maryland, College Park, MD 20742 (United States); Bobev, Svilen, E-mail: bobev@udel.edu [Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716 (United States)

    2014-03-15

    This study is concerned with the ternary compounds RELi{sub x}Sn{sub 2} (RE=La–Nd, Sm, and Gd; 0≤x<1), which have been previously thought to be the stoichiometric RELiSn{sub 2} phases. These materials crystallize with the base-centered orthorhombic space group Cmcm (No. 63), and can be formally assigned with the CeNiSi{sub 2} structure type (Pearson symbol oC16). Our systematic single-crystal X-ray diffraction studies revealed substantial Li-deficiencies in all cases, with SmSn{sub 2} (space group Cmmm, ZrGa{sub 2} structure type, Pearson symbol oC12) and GdSn{sub 2} (space group Cmcm, ZrSi{sub 2} structure type, Pearson symbol oC12) being completely lithium-free. The structure refinements also uncovered positional disorder on the Sn site neighboring the vacancies. The Sn-disorder and the Li-deficiency correlate, and vary monotonically with the decreased size of the rare-earth atoms in the order RE=La–Nd. The SmSn{sub 2} and GdSn{sub 2} structures are devoid of any disorder. Temperature-dependent studies of the magnetic response of the title compounds are also presented and discussed. -- Graphical abstract: RELi{sub x}Sn{sub 2} (RE=La–Nd, 0≤x<1) crystallize in a defect variants of the CeNiSi{sub 2} structure type (a). The Sn-disorder and the Li-deficiency correlate, and vary monotonically with the decreased size of the rare-earth atoms in the order RE=La–Nd. The SmSn{sub 2} (b) and GdSn{sub 2} (c) structures are devoid of any disorder. Highlights: • The crystal structures of the RELi{sub x}Sn{sub 2} (RE=La–Nd, 0≤x<1) compounds are revised using single-crystal X-ray diffraction data. • The structure is a filled derivative of the ZrSi{sub 2} structure type or defect variant of the CeNiSi{sub 2} structure type. • SmSn{sub 2} is isotypic with the ZrGa{sub 2} structure, while RESn{sub 2} (RE=Gd–Lu) are isotypic with the ZrSi{sub 2} structure.

  18. 依托博客重构大学英语专业写作评价模式%Reconstruction of Evaluation Model of Writing of English Major Relying on Blog

    Institute of Scientific and Technical Information of China (English)

    王晓南; 毛力

    2011-01-01

    Blog, as a new media, increasingly draws the attention of English educators. To explore the conjunction point of the blog and teaching evaluation of English writing, this paper discussed the reconstruction of writing evaluation model in English major relying on blog and designed out its relevant teaching plan.%博客作为一种新兴媒介日益引起英语教育者的关注,为探索博客与英语写作教学评价的契合点,本文探讨了依托博客重构大学英语专业写作评价模式并设计出了其相关教案.

  19. 依托食品专业社团创新工学结合人才培养模式%Relying on the food professional association to innovate the work-study combination talent training mode

    Institute of Scientific and Technical Information of China (English)

    张孔海; 豆成林; 魏明奎

    2014-01-01

    依托食品专业社团---食品科技协会,模拟企业运行机制,创新工学结合人才培养模式,实现教学做一体化、课内课外一体化、校内校外一体化,促进学生专业技能与综合素质的提高,开辟培养学生职业能力与素养的新途径。%Relying on a food professional association --- food science and technology association , simulating enter-prise’ s operating mechanism , innovating talent training mode of work -study combination , realizing integration of teach-ing, classroom curriculum integration and inside and outside university integration , improving students ’ professional and overall quality , and opening up a new way to train students ’ professional ability and quality .

  20. Does the Spatial Distribution of the Parasitic Mite Varroa jacobsoni Oud. (Mesostigmata: Varroidae) in Worker Brood of Honey Bee Apis Mellifera L. (Hymenoptera: Apidae) Rely on an Aggregative Process?

    Science.gov (United States)

    Salvy, M.; Capowiez, Y.; Le Conte, Y.; Salvy, M.; Clément, J.-L.

    Varroa jacobsoni is an ectoparasite of honey bees which reproduces in capped brood cells. Multi-infestation is frequently observed in worker brood and can be interpreted as an aggregative phenomenon. The aim of this study was to determine whether the distribution of V. jacobsoni in worker brood cells relies on a random or an aggregative process. We studied the distribution of Varroa females in capped worker brood at similar age by comparing, by a Monte Carlo test, the observed frequency distribution of mites per cell to simulated distributions based on a random process. A complementary approach, using the "nearest neighbor distances" (NND) with Monte Carlo tests, was investigated to study the spatial distribution (a) between mites in different cells and (b) between infested cells in brood. The observed distributions did not differ significantly from that expected by a random process, and we conclude that there is no aggregation during invasion of V. jacobsoni in worker brood.

  1. Spatial imagery relies on a sensory independent, though sensory sensitive, functional organization within the parietal cortex: a fMRI study of angle discrimination in sighted and congenitally blind individuals.

    Science.gov (United States)

    Bonino, Daniela; Ricciardi, Emiliano; Bernardi, Giulio; Sani, Lorenzo; Gentili, Claudio; Vecchi, Tomaso; Pietrini, Pietro

    2015-02-01

    Although vision offers distinctive information to space representation, individuals who lack vision since birth often show perceptual and representational skills comparable to those found in sighted individuals. However, congenitally blind individuals may result in impaired spatial analysis, when engaging in 'visual' spatial features (e.g., perspective or angle representation) or complex spatial mental abilities. In the present study, we measured behavioral and brain responses using functional magnetic resonance imaging in sighted and congenitally blind individuals during spatial imagery based on a modified version of the mental clock task (e.g., angle discrimination) and a simple recognition control condition, as conveyed across distinct sensory modalities: visual (sighted individuals only), tactile and auditory. Blind individuals were significantly less accurate during the auditory task, but comparable-to-sighted during the tactile task. As expected, both groups showed common neural activations in intraparietal and superior parietal regions across visual and non-visual spatial perception and imagery conditions, indicating the more abstract, sensory independent functional organization of these cortical areas, a property that we named supramodality. At the same time, however, comparisons in brain responses and functional connectivity patterns across experimental conditions demonstrated also a functional lateralization, in a way that correlated with the distinct behavioral performance in blind and sighted individuals. Specifically, blind individuals relied more on right parietal regions, mainly in the tactile and less in the auditory spatial processing. In sighted, spatial representation across modalities relied more on left parietal regions. In conclusions, intraparietal and superior parietal regions subserve supramodal spatial representations in sighted and congenitally blind individuals. Differences in their recruitment across non-visual spatial processing in

  2. 基于依托项目模式的商务英语专业实训研究与实践%Business English professional practical research and practice based on relying on the project model

    Institute of Scientific and Technical Information of China (English)

    冯巧丽

    2015-01-01

    作为一种以学习者为中心的教学方法,因其能够培养学生的创新能力、批判性思维的能力和合作学习的能力,依托项目模式在外语教学领域有着很大的发展潜力.本文以基于依托项目的商务英语专业实训课程为例,使用定性研究方法,结合定量数据,介绍了依托项目的语言实训教学的具体实施过程,并通过与传统教学方法的比较,指出其在学习者能力培养和反思,以及促进教师各方面提高等方面的特点与优势.%As a learner centered teaching methods, to cultivate students' innovation ability, critical thinking ability and cooperative learning ability, based on a project model has great potential for development in the field of foreign language teaching. In this paper, based on relying on the project of business English professional training courses, for example, using qualitative research methods, combined with the quantitative data, this paper introduces the implementation process of the relying on language training teaching project, and comparing with the traditional teaching methods, points out that the learners ability training and reflection, and improve teachers' various aspects.

  3. 依托网络媒体开展新时期大学生思想政治工作%Relying on Online Media to Carry Out the Ideological and Political Work

    Institute of Scientific and Technical Information of China (English)

    蓝振师; 朱家全; 韦容

    2012-01-01

    随着人类进入信息社会,网络以其独特的方式影响着社会各个领域,社会环境也发生了复杂而深刻的变化,思想政治工作面临着越来越多的困难和前所未有的挑战。本文从新时期对大学生思想政治工作的新要求出发,分析网络媒体在大学生思想政治工作中的作用,并提出了如何依托网络媒体开展大学生思想政治工作的开展。%As mankind enters the information society,the network in its own way affect all areas of society,the social environment has also undergone a complex and profound changes,more and more difficult and unprecedented challenges facing the ideological and political work.From the new requirements of the new era of ideological and political work,analysis of the role of online media in the ideological and political work,and how to rely on the network media to carry out ideological and political work carried out.

  4. Relying on Local Unique Advantages to Promote Transfer of Shaanxi Rural Labour%依托本地特色优势,促进陕西农村劳动力转移

    Institute of Scientific and Technical Information of China (English)

    钱黎阳; 楼旭明

    2011-01-01

    The transfer of rural labour is related to farmers' income, also is the basic premise of achieve social stability. This thesis, taking Shaanxi province as an example,presented the transfer of rural surplus labour should not only face industrial departments, but also rely on local unique advantages to achieve internal transfer, and developing the distinctive labor export, characteristic forestry, characteristic tourism and related special industry are the specific ways of transfer of rural surplus labour.%农村劳动力的转移关系到农民增收,也是实现社会稳定的一个基本前提.本文以西部省份-陕西省为例,从分析农村剩余劳动力形成的原因入手,认为农村剩余劳动力的转移不仅应面向工业部门,而且还应依托本地特色优势实现内部转移,其转移的具体方式为发展有特色的劳务输出、特色林业、特色旅游业和相关的特色产业.

  5. 依托住宅产业化开发蒸压砂加气混凝土成套体系技术%Exploitation of Complete Sets of Sand Autoclaved Aerated Concrete System Technology Relying on the Housing Industrialization

    Institute of Scientific and Technical Information of China (English)

    王超华

    2015-01-01

    Relying on the advantage of group industrialization to develop research and development and application of sand autoclaved aerated concrete materials for the maintenance system of the green, environmental protection,energy saving technology,formed sets of system technology of the sand autoclaved aerated insulation,exterior insulation,partition panel,steel structure within the envelope insulation panels etc.,then summarized the construction method of complete system.%依托集团产业化优势,大力开展以蒸压砂加气混凝土材料为建筑围护体系的绿色、环保、节能技术研发与应用,形成了蒸压砂加气自保温、外墙外保温、內隔墙板、钢结构外围护保温墙板等多项成套体系技术,进而总结提炼出成套体系施工工法。

  6. An Investigation on the impact of using problem-based trainings in the in-service courses on the teachers' performance and capabilities, by relying on the elementary sixth grade mathematics book (case study: the city of Ahvaz

    Directory of Open Access Journals (Sweden)

    Pari Cheraq

    2017-02-01

    Full Text Available Changes in the administrative structure of the education system and the need to coordinate with the world of science and technology and hence, the need to update the general and technical information and knowledge, have doubled the importance and necessity of holding the new and efficient in-service trainings for the teachers. In this study, the attempt was taken to examine and analyze the impact of using problem-based trainings during the in-service courses on the teachers' performance and capabilities, by relying on the mathematics book of the sixth grade elementary level (case study: the city of Ahvaz. To do so, using a quasi-experimental study, 37 teachers of the sixth grade were selected from the elementary schools of Ahvaz city. For the in-service training courses on the teaching of mathematics, the problem-based learning method was used. After conducting the problem-based teaching method and the traditional method of teaching, through using the test and the standard questionnaire, the data about the teaching practice and the capabilities of the teachers were collected. Using t-test for the paired and independent samples, it was demonstrated that the problem-based in-service trainings, enhance the teachers' capabilities and performance regarding the problem-solving skills.

  7. Cultivation Full-time Professional Master Relying on the Public Laboratory%依托公共实验基地培养全日制专业硕士

    Institute of Scientific and Technical Information of China (English)

    姚睿; 王友仁; 陈则王; 周翟和; 万晓冬

    2014-01-01

    In this paper,instructional and teaching methodologies to cultivate applied cross-disciplinary full-time professional master have been explored relying on the graduate public experimental teaching base. Further reforms have been carried on the curriculum system and teaching content of the curriculum set of high and new technology in automation field,such as DSP,ARM,MCU,FPGA and PLC,etc.;meanwhile,teaching model,teaching method as well as the opening mechanism of the public experimental teaching base have also been explored,so as to train the capabilities of the engineering application,and science and technology of full-time professional master.%本文依托研究生公共实验教学基地,以自动化领域高新技术工程应用与科技创新能力培养为抓手,探索应用复合型全日制专业硕士生的教育教学方法;对DSP、ARM、单片机、FPGA和PLC等自动化领域高新技术系列课程的课程体系与教学内容、教学模式与教学方法,以及公共实验基地的开放机制进行了深入改革,取得了良好效果。

  8. Establishment of a Predictive In Vitro Assay for Assessment of the Hepatotoxic Potential of Oligonucleotide Drugs

    Science.gov (United States)

    Sewing, Sabine; Boess, Franziska; Moisan, Annie; Bertinetti-Lapatki, Cristina; Minz, Tanja; Hedtjaern, Maj; Tessier, Yann; Schuler, Franz; Singer, Thomas; Roth, Adrian B.

    2016-01-01

    Single stranded oligonucleotides (SSO) represent a novel therapeutic modality that opens new space to address previously undruggable targets. In spite of their proven efficacy, the development of promising SSO drug candidates has been limited by reported cases of SSO-associated hepatotoxicity. The mechanisms of SSO induced liver toxicity are poorly understood, and up to now no preclinical in vitro model has been established that allows prediction of the hepatotoxicity risk of a given SSO. Therefore, preclinical assessment of hepatic liability currently relies on rodent studies that require large cohorts of animals and lengthy protocols. Here, we describe the establishment and validation of an in vitro assay using primary hepatocytes that recapitulates the hepatotoxic profile of SSOs previously observed in rodents. In vitro cytotoxicity upon unassisted delivery was measured as an increase in extracellular lactate dehydrogenase (LDH) levels and concomitant reduction in intracellular glutathione and ATP levels after 3 days of treatment. Furthermore, toxic, but not safe, SSOs led to an increase in miR-122 in cell culture supernatants after 2 days of exposure, revealing the potential use of miR122 as a selective translational biomarker for detection of SSO-induced hepatotoxicity. Overall, we have developed and validated for the first time a robust in vitro screening assay for SSO liver safety profiling which allows rapid prioritization of candidate molecules early on in development. PMID:27442522

  9. Establishment of a Predictive In Vitro Assay for Assessment of the Hepatotoxic Potential of Oligonucleotide Drugs.

    Directory of Open Access Journals (Sweden)

    Sabine Sewing

    Full Text Available Single stranded oligonucleotides (SSO represent a novel therapeutic modality that opens new space to address previously undruggable targets. In spite of their proven efficacy, the development of promising SSO drug candidates has been limited by reported cases of SSO-associated hepatotoxicity. The mechanisms of SSO induced liver toxicity are poorly understood, and up to now no preclinical in vitro model has been established that allows prediction of the hepatotoxicity risk of a given SSO. Therefore, preclinical assessment of hepatic liability currently relies on rodent studies that require large cohorts of animals and lengthy protocols. Here, we describe the establishment and validation of an in vitro assay using primary hepatocytes that recapitulates the hepatotoxic profile of SSOs previously observed in rodents. In vitro cytotoxicity upon unassisted delivery was measured as an increase in extracellular lactate dehydrogenase (LDH levels and concomitant reduction in intracellular glutathione and ATP levels after 3 days of treatment. Furthermore, toxic, but not safe, SSOs led to an increase in miR-122 in cell culture supernatants after 2 days of exposure, revealing the potential use of miR122 as a selective translational biomarker for detection of SSO-induced hepatotoxicity. Overall, we have developed and validated for the first time a robust in vitro screening assay for SSO liver safety profiling which allows rapid prioritization of candidate molecules early on in development.

  10. Ege bölgesi standart süreli yıllık maksimum yağışları için en uygun dağılımlar

    Directory of Open Access Journals (Sweden)

    Halil Karahan

    2013-03-01

    Full Text Available Su kaynaklarının temel girdisi olan yağışların; miktarı, süresi, şiddeti, alansal ve zamansal değişimi v.b. özelliklerinin bilinmesi; su kaynakları, tarım, kentleşme, drenaj, taşkın kontrolü ve ulaşım gibi farklı sektörlere ait planlama, tasarım, inşaat ve işletme çalışmaları için gereklidir. Belirtilen faaliyetlerin gerçekleştirilebilmesi için; mevcut gözlemlere dayalı, güvenilir ve gerçekçi tahminlerin yapılması gerekir. Güvenilir tahmin yapabilmenin ilk aşaması ise mevcut gözlemlerin güvenirliğinin test edilmesidir. Bu çalışmada; DMİ Genel Müdürlüğü tarafından işletilen Ege Bölgesi il ve ilçe merkezlerinde yer alan meteoroloji istasyonlarında (1929-2005 yıllarında ölçülen standart süreli maksimum yağış değerlerinin hangi dağılıma uyduklarını belirlemek için; Kolmogorov-Smirnov, Anderson-Darling ve Ki-Kare dağılım uygunluk testleri uygulanmıştır. Anderson-Darling testine göre gözlemlerin tümüne yakını GEV dağılımına uyum gösterirken, Kolmogorov-Smirnov ve Ki-Kare testlerine göre kısa, orta ve uzun süreli yağış gözlemleri için genellikle GEV, Gamma ve Log-Normal dağılımın uygun olduğu görülmüştür. Seçilen olasılık dağılımının parametrelerini belirlemek için farklı dağılımlara göre maksimum olabilirlik (LN2, LN3, EXP2, Gamma3, olasılık-ağırlıklı momentler (LP3,Gamma2, L-momentler (GEV ve en küçük kareler (Weibull2 yöntemleri kullanılmıştır.

  11. Tackle“Three New Dimensional Rural Issues” Relying on Innovation-Driven Development Strategy%依靠创新驱动发展战略发展“新三农”

    Institute of Scientific and Technical Information of China (English)

    张来武

    2014-01-01

    The article first explores the backgrounds of bringing forward “Four New Modernization” and“Four Moderni-zations Synchronization”,pointing out that agricultural modernization is the base of industrialization ,informatization and urbanization.Then the article analyses the challenges in the process of China's agricultural modernization in terms of re-sources and environments , market demands , production factors , as well as organization models , putting forward that we must rely on innovation-driven development strategy to tackle “Three New Dimensional Rural Issues” -“modern new agriculture,urbanized new countryside and professional new farmer”.Finally the article discusses how to handle “Three New Dimensional Rural Issues” depending on innovation-driven development strategy , combining with the practice of Technical Task Force ( TTF) system and rural sci-tech entrepreneurship.%本文首先分析了“新四化”以及“四化同步”提出的背景,认为农业现代化是工业化、信息化和城镇化的基础和支撑;随后从资源环境、市场需求、生产要素和组织模式等方面分析了新时期我国农业现代化面临的挑战,提出要依靠创新驱动发展战略发展“新三农”---现代化的新农业、城镇化的新农村以及职业化的新农民;最后结合科技特派员制度和农村科技创业实践,探讨了新时期如何依靠创新驱动发展战略发展“新三农”。

  12. Suggestions for Development of High-end Fine Quality Agriculture by Relying on Scientific and Technological Innovation%依靠科技创新促精品高端农业发展的建议

    Institute of Scientific and Technical Information of China (English)

    温淑萍

    2014-01-01

    介绍了精品高端农业概念的发展,分析了发展精品高端农业的必要性及现存共性问题,提出依靠科技创新,促精品高端农业发展的建议。并提出各类农产品的加工标准及其信息化、自动化生产线成套设备设施的研发是精品高端农业科技创新的一个重要方向;提出精品高端农业发展的一个主流模式是合理布局农产品区域加工中心企业,以此关联产业协会及农产品生产主体,形成“初级农产品由生产主体按相关标准生产-加工产品统一质量规格标准经生产线加工包装-商品区域品牌市场化联营联销-产品质量主要由产业协会全程追溯监管”的品牌化生产模式。%A discussion is made on the concept of the high-end fine quality agriculture. The necessity and existing common problems in the development of the high-end fine quality agriculture are elaborated. Some suggestions for promoting the development of the high-end fine quality agriculture by relying on scientific and technological innovation are put forward. It is also put forward that the R&D of various agriculture products process standards and their informationized and automatic process equipment and facilities are the main direction of scientific and technological innovation in the aspect of the high-end fine quality agriculture. A mainstream pattern for development of the high-end fine quality agriculture is to arrange the regional core agricultural products process enterprises in this way that the agriculture industry association and the agricultural products producing bodies are combined together and consist a brand products process pattern: primary agricultural products produced by the farmer or agricultural company according to the standards, made products processed and packaged by processing lines according to the unified quality standards, the commodity labeled local brands sale in market by chain stores, the quality of the products is

  13. 依托冀中南经济区优势促进邯郸产业发展%Relying on ji-zhong-nan economic zone advantage to promote the handan industry development

    Institute of Scientific and Technical Information of China (English)

    孙健夫; 宁志玲

    2014-01-01

    Handan is a national famous city with long history and culture. It is located in the southern part of hebei province, shanxi-hebei-shandong-henan area over the four provinces and the central plains economic zone centre, Handan is an important part in the hebei economic development due to its rich mineral resources. Relying on its unique advantages, the economy develops constantly. But in the new situation, its economic development is faced with many difficulties and challenges. This article is mainly about handan industry development and its pillar industry based on the advantage of resources. Under the beijing-tianjin-hebei regional integration background, there are lots ofopportunities and challenges for handan to adjust its industrial structure. Moreover, it points out how to transform and upgrade the handan’s industrial structure in the future. Through analysis of the handan’s industry problem, I hope that it can provide certain reference to the handan industry development for the future.%邯郸市是国家历史文化名城,地处河北省南部,晋冀鲁豫四省交界和中原经济区腹心,矿产资源丰富,是河北经济发展的重要力量,依据其独特的优势,经济不断发展。但是在新形势下,邯郸经济发展也面临着许多困难与挑战。文章主要从邯郸经济发展拥有的优势、基于优势建立起来的支柱产业,融合了京津冀一体化大背景下,邯郸进行产业结构调整面临的机遇与挑战以及未来邯郸应当如何转型升级发展等问题展开分析。

  14. EPO relies upon novel signaling of Wnt1 that requires Akt1, FoxO3a, GSK-3β, and β-catenin to foster vascular integrity during experimental diabetes.

    Science.gov (United States)

    Chong, Zhao Zhong; Hou, Jinling; Shang, Yan Chen; Wang, Shaohui; Maiese, Kenneth

    2011-05-01

    caspase activation during elevated D-glucose. Our studies identify critical elements of the protective cascade for EPO that rely upon modulation of Wnt1, Akt1, FoxO3a, GSK-3β, β-catenin, and mitochondrial apoptotic pathways for the development of new strategies against DM vascular complications.

  15. 依托家教网的电子学习档案的设计和有效使用%The Design and Effective Use of Electronic Learning Files Relying on Tutor Nets

    Institute of Scientific and Technical Information of China (English)

    陈曼曼; 杨小娟

    2013-01-01

    Based on the currently popular tutors network, the pa-per studies the entry of its present situation, the background and significance. It analyzes the importance of electronic learning archives in recording and evaluating students' learning process, and finds the possibility of combining electronic learning archives with tutoring nets. Through various experimental investigation and analysis of literature, this paper puts forward the module function of electronic learning archives relying on the tutoring nets and supported by various learning theories, and eventually establishes the modules of electronic learning archives based on theory and practice investigation, and explains the deficiency in its use and practice. It is of profound significance to continuously research electronic learning archives in the background of further development of individualized education.%  本文以现在流行的家教网为切入点进行分析,研究了其现状、背景和意义。对电子学习档案在学生学习过程中的重要记录评价作用进行分析,研究发现电子学习档案和家教网相结合的可能性。通过各种实验调查,文献分析研究,提出依托家教网的电子学习档案在各种学习理论支持下应该具有的模块功能,最终依托理论和实践调查,建立起电子学习档案的各个模块,并且对其使用和实践中的不足给以说明。在个别化教育深入发展的同时对依托家教网的电子学习档案的不断研究意义深远。

  16. Performance and appraisal assessment integrated system for management of nosocomial infections relying on informatization%依托信息化手段构建医院感染绩效考核一体化管理体系

    Institute of Scientific and Technical Information of China (English)

    黄荔红; 吴鲤霞; 王佳; 刘玉光; 游荔君

    2012-01-01

    OBJECTIVE To explore a scientific, objective, efficient hospital infection management new pattern, improve the quality of hospital infection management. METHODS Based on computer network technology, a performance assessment of integrated management system of hospital infection was developed. The incidence of . hospital infections, pathogen detection rate, hospital infection knowledge training participation rates, antibiotic use rates in inpatients, liquid soap and disinfectant hand consumption ratio were compared through the application of performance appraisal of integrated management system. RESULTS Before the use of performance appraisal of integrated management system, the hospital infection knowledge training participation rates of the medical staff and the submission rate of the pathogens were 91. 30% and 34. 87%, respectively, which were 97. 80% and 47.98% after the use of the system, the consumption of the hand disinfectants and the sanitizer increased significantly; the incidence of nosocomial infections and the utilization rate of antibiotics were 3. 78% and 58. 93% before the use of system and were 2. 20% and 43. 72% after the application, the differences were statistically significant (P<0.05). CONCLUSION The application of hospital infections of performance appraisal management integrated system relying on the informatization can effectively improve the whole quality of hospital infection management and management efficiency.%目的 探索一种科学、客观、高效的医院感染管理新模式,提高医院感染管理质量.方法 依托计算机网络技术研发一套医院感染绩效考核一体化管理体系,医院感染管理对应用医院感染绩效考核一体化管理体系前后医院感染发病率、病原送检率、医院感染知识培训参学率、住院患者抗菌药物使用率、洗手液和手消毒剂消耗量进行比效.结果 在应用绩效考核一体化管理体系前医务人员医院感染知识培训参学率

  17. Nursing care for patients with copd on long-term oxygen therapyUzun süreli oksijen tedavisi alan koah hastalarında hemşirelik bakımı

    Directory of Open Access Journals (Sweden)

    Türkan Karaca

    2016-05-01

    çinde kronik solunum yolu hastalıkları, kalp damar hastalıkları, kanserler ve diyabet ile beraber dünyanın en büyük katilleri olup, her yıl tahminen 35 milyon ölüme neden olmaktadır.  Bu verilere ek olarak, dünyada ölüm nedeni sıralamasında solunum sistemi hastalıklarından, KOAH dördüncü sırada yer almaktadır. Ayrıca bu raporda, 2030 yılı için öngörülen ölüm nedeni sıralamasında, KOAH’ın üçüncü sıraya yükseleceği tahmin edilmektedir (DSÖ, 2010.   Teknolojide ve sağlık alanında ki gelişmeler, hastalıklara erken tanı koymasına ve hastalık durumunda en uygun tedavi yöntemini uygulamasına olanak sağlamaktadır. KOAH’da en sık kullanılan tedavi yöntemi uuzun süreli oksijen tedavisidir. Bu nedenle hemşirelerin kaliteli bir hemşirelik bakımı vermeleri için, oksijen tedavisinin temel kavramlarını, uygulama yöntemlerini, gelişebilecek komplikasyonları bilmesi önemlidir.

  18. The Transfer of Acquired Spatial Associations Relies on Verbal Working Memory%习得性空间联结的迁移依赖于语义工作记忆

    Institute of Scientific and Technical Information of China (English)

    王力; 陈安涛

    2012-01-01

    employment of Chinese characters in the verbal WM load task could be problematical, since it has been demonstrated that Chinese characters may engage spatial processing in addition to verbal processing; on the other hand, pseudo-characters may have some linguistic properties. Therefore, it is premature to conclude that the acquired associations are represented as verbal codes in WM. In order to address the limitations of Experiment 1, in Experiment 2, the stimuli of WM load task were the Chinese characters presented through auditory modality in the verbal WM load task and the pseudo-characters were replaced with black filled squares. The characteristics of these stimuli may ensure that the verbal WM load and spatial WM load occupied the phonological loop and the visuospatial sketchpad of the working memory, respectively. The results showed significant reversal Simon effects in the spatial dual-task, but no normal or reversal Simon effect was observed in the verbal dual-task, which perfectly accords with Experiment 1. Hence, we can confirm that the acquired associations are represented as verbal codes in WM. Straightforwardly, the present study strongly suggested that the transfer of acquired associations relies on the verbal working memory.%采用练习迁移范式与双任务范式相结合的设计来探讨练习所习得的空间联结在工作记忆中如何表征.被试先进行不一致空间的刺激一反应映射练习任务,五分钟后,随机迁移到单任务(Simon任务)或者双任务(Simon任务+语义工作记忆负荷任务或空间工作记忆负荷任务).结果发现:不一致的练习能使单任务出现反转的Simon效应,但语义工作记忆负荷会使反转的Simon效应消失,而空间工作记忆负荷却对反转的Simon效应没有影响.实验结果表明练习产生的空间联结依赖于语义工作记忆.

  19. Comparative study of enamel adhesion between RelyX™ Unicem® (3M), a self-adhesive bonding agent, and the combination of MIP® (3M), a hydrophilic adhesive, and Transbond Supreme Low Viscosity® (3M), a traditional hydrophobic adhesive.

    Science.gov (United States)

    Dubernard, Charles; Raynal, Perrine; Tramini, Paul

    2013-09-01

    Although the bond strength of self-adhesive bonding agents is inferior to that of other families of adhesives, it is still adequate for orthodontic purposes provided prior enamel etching is performed. To determine the efficacy of RelyX™ Unicem(®) (3M) self-adhesive cement both in vitro and in vivo and to compare it with the combination of MIP(®) (3M), a moisture-insensitive primer, with a traditional hydrophobic adhesive, Transbond Supreme Low Viscosity(®) (3M). Comparison of bonding results on 23 trial dentures using RelyX™ Unicem(®) (3M) with bonding results on 29 trial dentures using a combination of MIP(®) and Transbond Supreme Low Viscosity(®) (3M), by means of a multipurpose Instron(®) 4444 testing machine. the breaking force of MIP(®)/Transbond Supreme Low Viscosity(®) (3M) (mean: 144±37.5 Newtons) was significantly higher than that of RelyX™ Unicem(®) (3M) (mean=110±26 Newtons) (P=0.001). A 12-month prospective, randomized, monocentric, single-blind clinical study in order to investigate the failure rate of orthodontic attachments according to the type of adhesive used, and the precise site of the debonding. Bracket bonding was performed on 16 patients with randomized allocation of the two adhesives to each of the semi-arches. The failure rates were: 15.3% for the MIP(®)/Transbond Supreme Low Viscosity(®) (3M) combination and 8.2% for the RelyX™ Unicem(®) (3M), with a significant difference (P=0.039). The more posterior the bonded teeth, the greater the superiority of RelyX™ Unicem(®) (3M). The in vivo results did not concord with those obtained in vitro. RelyX™ Unicem(®) (3M) exhibited lower adhesion values in vitro and yet it presented a debonding rate almost half that of the MIP(®)/Transbond Supreme Low Viscosity(®) (3M). The viscosity of RelyX™ Unicem(®) (3M) and its moisture tolerance would appear to account for these results. With prior etching, RelyX™ Unicem(®) (3M), a self-adhesive, self-etching bonding

  20. Evaluation of the Use of Periodicals Collection of the Higher Education Council Documentation and Online Search Center: Arts and Humanities Periodicals YÖK Dokümantasyon ve Uluslararası Bilgi Tarama Merkezi Koleksiyonundaki Sanat ve Beşeri Bilimler Konulu Süreli Yayınların Kullanım Değerlendirmesi

    Directory of Open Access Journals (Sweden)

    Elçin Özbudak

    1995-06-01

    Full Text Available Evaluation of the use perodicals collections in libraries is important as perodicals report the results of the latest scientific research and they are usually more expensive than other types of library materials. In this study we evaluate the use of Arts and Humanities periodicals in the collection of the Higher Education Council Documentation Center. Although the use of Arts and Humanities periodicals was found to be lower than, say, Biomedical periodicals collection, the number of journal titles in Arts and Humanities appears to be quite satisfactory. Some suggestions are made so as to increase the use of Arts and Humanities periodicals collection. Bilimsel araştırmalar için en güncel bilgileri veren ve maliyeti diğer materyallere göre daha fazla olan süreli yayınların ne oranda kullanıldığının araştırılması, koleksiyon değerlendirmesi açısından önemlidir. Bu amaçla, Yükseköğretim Kurulu Dokümantasyon ve Uluslararası Bilgi Tarama Merkezi süreli yayınlar koleksiyonunun genel bir değerlendirmesi yapılarak "Sanat ve Beşeri Bilimler" konulu süreli yayınların kullanımları incelenmiş, Yükseköğretim Kurulu Dokümantasyon ve Uluslararası Bilgi Tarama Merkezi 'nde önemli ve büyük bir süreli yayınlar koleksiyonu bulunduğu, "Sanat ve Beşeri Bilimler" konulu süreli yayınların da yeterli olduğu sonuçları çıkartılmıştır. "Sanat ve Beşeri Bilimler" konulu süreli yayınların kullanımlarının nispeten düşük oluşu, bu alanda çalışan araştırmacıların süreli yayınlara yeterince ilgi göstermemelerine bağlanarak, kullanımı artırmak için öneriler getirilmiştir.

  1. Sample Subset Optimization Techniques for Imbalanced and Ensemble Learning Problems in Bioinformatics Applications.

    Science.gov (United States)

    Yang, Pengyi; Yoo, Paul D; Fernando, Juanita; Zhou, Bing B; Zhang, Zili; Zomaya, Albert Y

    2014-03-01

    Data sampling is a widely used technique in a broad range of machine learning problems. Traditional sampling approaches generally rely on random resampling from a given dataset. However, these approaches do not take into consideration additional information, such as sample quality and usefulness. We recently proposed a data sampling technique, called sample subset optimization (SSO). The SSO technique relies on a cross-validation procedure for identifying and selecting the most useful samples as subsets. In this paper, we describe the application of SSO techniques to imbalanced and ensemble learning problems, respectively. For imbalanced learning, the SSO technique is employed as an under-sampling technique for identifying a subset of highly discriminative samples in the majority class. In ensemble learning, the SSO technique is utilized as a generic ensemble technique where multiple optimized subsets of samples from each class are selected for building an ensemble classifier. We demonstrate the utilities and advantages of the proposed techniques on a variety of bioinformatics applications where class imbalance, small sample size, and noisy data are prevalent.

  2. An efficient circle detector not relying on edge detection

    Science.gov (United States)

    Cai, Jia; Huang, Panfeng; Chen, Lu; Zhang, Bin

    2016-06-01

    Accurate and efficient detection of circular modules fixed on non-cooperative target is a key technology for Tethered Space Robot. This paper presents an efficient circle detector based on region-growing of gradient and histogram distribution of Euclidean distance. Region-growing of gradient is applied to generate arc support regions from single point. And the corresponding square fitting areas are defined to accelerate the detection and decrease storage. A histogram is then used to count frequency of the distances that participates in the accumulator and the parameters of each circle are acquired. Finally, a verification strategy of circular integrity is designed to test the detection results. We have tested our algorithm on 35 images dealing with kinds of circles and ellipses. Experimental results demonstrate that our method is able to detect circular objects under occlusion, image noises and moderate shape deformations with a good precision.

  3. Does avian magnetoreception rely on both magnetite and maghemite?

    CERN Document Server

    Winklhofer, Michael

    2008-01-01

    Recently, a putative magnetoreceptor in the upper-beak skin of homing pigeons was chemically characterized using X-ray flourescence and absorbtion studies [Fleissner et al., 2007. A novel concept of Fe-mineral based magnetoreception: histological and physiochemical data from the upper beak of homing pigeons. Naturwissenschaften 94 (8), 631-642]. In this short communication we point out that the novel concept propagated is based on conclusions that are not supported by data and, in fact, stand in contradiction to previously published crystallographic and magnetic data.

  4. Towards Concept Understanding relying on Conceptualisation in Constructivist Learning

    DEFF Research Database (Denmark)

    Badie, Farshad

    2017-01-01

    , through this constructivism to a pedagogical theory of learning. I will mainly focus on conceptual and epistemological analysis of humans’ conceptualisations based on their own mental objects (schemata). Subsequently, I will propose an analytical specification of humans’ conceptualisations...... and understandings over their mental structures in the framework of constructivism, and I will clarify my logical [and semantic] conceptions of humans’ concept understandings. This research focuses on philosophy of education and on logics of human learning. It connects with the topics ‘Cognition in Education...

  5. Towards Concept Understanding relying on Conceptualisation in Constructivist Learning

    DEFF Research Database (Denmark)

    Badie, Farshad

    2017-01-01

    This research works within the framework of constructivist learning (based on constructivist epistemology) and examines learning as an activity of construction, and it posits that knowledge acquisition (and learning) are transformative through self-involvement in some subject matter. Thus it lead...

  6. Why cryptography should not rely on physical attack complexity

    CERN Document Server

    Krämer, Juliane

    2015-01-01

    This book presents two practical physical attacks. It shows how attackers can reveal the secret key of symmetric as well as asymmetric cryptographic algorithms based on these attacks, and presents countermeasures on the software and the hardware level that can help to prevent them in the future. Though their theory has been known for several years now, since neither attack has yet been successfully implemented in practice, they have generally not been considered a serious threat. In short, their physical attack complexity has been overestimated and the implied security threat has been underestimated. First, the book introduces the photonic side channel, which offers not only temporal resolution, but also the highest possible spatial resolution. Due to the high cost of its initial implementation, it has not been taken seriously. The work shows both simple and differential photonic side channel analyses. Then, it presents a fault attack against pairing-based cryptography. Due to the need for at least two indepe...

  7. Unlocking good design does not rely on designers alone

    DEFF Research Database (Denmark)

    Rodil, Kasper; Eskildsen, Søren; Morrison, Ann;

    This article describes on-going research in the design of sustainable systems to support transfer of indigenous knowledge between rural elders and city living youths in Namibia. Specifically we here address the importance of co-design and highlight some of our findings through experiences gathere...

  8. The Failing Heart Relies on Ketone Bodies as a Fuel.

    Science.gov (United States)

    Aubert, Gregory; Martin, Ola J; Horton, Julie L; Lai, Ling; Vega, Rick B; Leone, Teresa C; Koves, Timothy; Gardell, Stephen J; Krüger, Marcus; Hoppel, Charles L; Lewandowski, E Douglas; Crawford, Peter A; Muoio, Deborah M; Kelly, Daniel P

    2016-02-23

    Significant evidence indicates that the failing heart is energy starved. During the development of heart failure, the capacity of the heart to utilize fatty acids, the chief fuel, is diminished. Identification of alternate pathways for myocardial fuel oxidation could unveil novel strategies to treat heart failure. Quantitative mitochondrial proteomics was used to identify energy metabolic derangements that occur during the development of cardiac hypertrophy and heart failure in well-defined mouse models. As expected, the amounts of proteins involved in fatty acid utilization were downregulated in myocardial samples from the failing heart. Conversely, expression of β-hydroxybutyrate dehydrogenase 1, a key enzyme in the ketone oxidation pathway, was increased in the heart failure samples. Studies of relative oxidation in an isolated heart preparation using ex vivo nuclear magnetic resonance combined with targeted quantitative myocardial metabolomic profiling using mass spectrometry revealed that the hypertrophied and failing heart shifts to oxidizing ketone bodies as a fuel source in the context of reduced capacity to oxidize fatty acids. Distinct myocardial metabolomic signatures of ketone oxidation were identified. These results indicate that the hypertrophied and failing heart shifts to ketone bodies as a significant fuel source for oxidative ATP production. Specific metabolite biosignatures of in vivo cardiac ketone utilization were identified. Future studies aimed at determining whether this fuel shift is adaptive or maladaptive could unveil new therapeutic strategies for heart failure. © 2016 American Heart Association, Inc.

  9. Mayaro virus infection cycle relies on casein kinase 2 activity.

    Science.gov (United States)

    Barroso, Madalena M S; Lima, Carla S; Silva-Neto, Mário A C; Da Poian, Andrea T

    2002-09-06

    Replication of Mayaro virus in Vero cells induces dramatic cytopathic effects and cell death. In this study, we have evaluated the role of casein kinase 2 (CK2) during Mayaro virus infection cycle. We found that CK2 was activated during the initial stages of infection ( approximately 36% after 4h). This activation was further confirmed when the enzyme was partially purified from the cellular lysate either by Mono Q 5/5Hr column or heparin-agarose column. Using this later column, we found that the elution profile of CK2 activity from infected cells was different from that obtained for control cell enzyme, suggesting a structural modification of CK2 after infection. Treatment of infected cells with a cell-permeable inhibitor of CK2, dichloro-1-(beta-D-ribofuranosyl)benzimidazole (DRB), abolished the cytopathic effect in a dose-dependent manner. Together this set of data demonstrates for the first time that CK2 activity in host cells is required in Mayaro virus infection cycle.

  10. Hepatitis C virus relies on lipoproteins for its life cycle.

    Science.gov (United States)

    Grassi, Germana; Di Caprio, Giorgia; Fimia, Gian Maria; Ippolito, Giuseppe; Tripodi, Marco; Alonzi, Tonino

    2016-02-14

    Hepatitis C virus (HCV) infects over 150 million people worldwide. In most cases, HCV infection becomes chronic causing liver disease ranging from fibrosis to cirrhosis and hepatocellular carcinoma. Viral persistence and pathogenesis are due to the ability of HCV to deregulate specific host processes, mainly lipid metabolism and innate immunity. In particular, HCV exploits the lipoprotein machineries for almost all steps of its life cycle. The aim of this review is to summarize current knowledge concerning the interplay between HCV and lipoprotein metabolism. We discuss the role played by members of lipoproteins in HCV entry, replication and virion production.

  11. Pallet industry relying more on recovered wood material

    Science.gov (United States)

    J. Bejune; R. Bush; Philip A. Araman; B. Hansen; D. Cumbo

    2002-01-01

    This article is a research report titled, Wood Use Trends in the Pallet and Container Industry: 1992 - 1999, that was published by the authors earlier this year. The research was conducted by Virginia Tech in collaboration with the U.S. Forest Service Southern Research Station. Part one, published in the September, 2002 issue of Pallet Enterprise, summarized the...

  12. Legal research when relying on open access: a primer

    NARCIS (Netherlands)

    van Dijck, Gijs

    2016-01-01

    With more and more information disclosed online and with open-access policies on the rise, legal academic research is becoming more accessible. The potential impact of this development is enormous, particularly in areas or jurisdictions where offline information is scarce and where access to subscri

  13. A Connectionist Treatment of Grammar for Generation: Relying on Emergents

    Science.gov (United States)

    1990-01-01

    specifically, ( Stemberger 1985) suggested that human speakers can be modeled as having many ’phrase structure units’ being ’partially activated’ si...Second European Workshop on Natural Language Generation. Stemberger , J. P. (1985). An Interactive Activation Model of Language Production. In

  14. Reinforcement learning in multidimensional environments relies on attention mechanisms.

    Science.gov (United States)

    Niv, Yael; Daniel, Reka; Geana, Andra; Gershman, Samuel J; Leong, Yuan Chang; Radulescu, Angela; Wilson, Robert C

    2015-05-27

    In recent years, ideas from the computational field of reinforcement learning have revolutionized the study of learning in the brain, famously providing new, precise theories of how dopamine affects learning in the basal ganglia. However, reinforcement learning algorithms are notorious for not scaling well to multidimensional environments, as is required for real-world learning. We hypothesized that the brain naturally reduces the dimensionality of real-world problems to only those dimensions that are relevant to predicting reward, and conducted an experiment to assess by what algorithms and with what neural mechanisms this "representation learning" process is realized in humans. Our results suggest that a bilateral attentional control network comprising the intraparietal sulcus, precuneus, and dorsolateral prefrontal cortex is involved in selecting what dimensions are relevant to the task at hand, effectively updating the task representation through trial and error. In this way, cortical attention mechanisms interact with learning in the basal ganglia to solve the "curse of dimensionality" in reinforcement learning.

  15. Intermediation in Foreign Trade: When do Exporters Rely on Intermediaries?

    DEFF Research Database (Denmark)

    Schröder, Philipp J.H.; Trabold, H.; Trübswetter, P.

    2005-01-01

    The paper explores the question of why trade intermediaries (TIs) are frequently used as agents for exports to some countries but not to others. First, we adapt a standard intra-industry trade model with variable export costs (e.g. transport) and fixed export costs (e.g. market access) to include...... a TI that is able to pool market access cost. This framework suggests explanatory factors for the TI share in a country's exports, which are largely in line with the literature. Second, we test these explanatory factors with a new data set based on French customs information. The paper finds that: (i......) higher market access costs increase the TI share, (ii) smaller export markets feature a larger TI share, (iii) network effects are important determinants of trade intermediation....

  16. Intermediation in Foreign Trade: When do Exporters Rely on Intermediaries?

    DEFF Research Database (Denmark)

    Schröder, Philipp; Trabold, Harald; Trübswetter, Parvati

    2003-01-01

    The paper explores theoretically and empirically why trade intermediaries (TIs) are frequently used as agents for exports to some countries but not to others. We adapt a standard intra-industry trade model with variable export costs (e.g. transport) and fixed export costs (e.g. market access......) to include a TI that is able to pool market access cost. From this framework explanatory factors for the TI share in a country's exports are derived and subsequently tested with a new data set based on French customs information. The paper finds that: (i) higher market access costs increase the TI share, (ii......) smaller export markets feature a larger TI share, (iii) the TI share is independent from variable (distance-dependent) export costs....

  17. Legal research when relying on open access : A primer

    NARCIS (Netherlands)

    van Dijck, Gijs

    2016-01-01

    With more and more information disclosed online and with open-access policies on the rise, legal academic research is becoming more accessible. The potential impact of this development is enormous, particularly in areas or jurisdictions where offline information is scarce and where access to subscri

  18. Une herbe qui relie les parties du monde…

    Directory of Open Access Journals (Sweden)

    Louise Bénat-Tachot

    2011-06-01

    Full Text Available Le livre 50 de la Historia natural y moral de las Indias est la conclusion de trente-cinq années d’expérience américaine, de compilation de récits, de témoignages, d’entrevues dont Gonzalo Fernandez de Oviedo se fait le garant. Peu étudié et surtout classé comme une forme littéraire de l’histoire où intervient le miraculeux, cet ensemble de 19 chapitres présente néanmoins un intérêt singulier pour comprendre le monde colonial et l’importance que la navigation comme expérience commune partagée a pu avoir dans les sociétés en voie de constitution. Ce livre n’est donc pas simplement une vision assombrie de l’Amérique, aux antipodes de l’utopie, mais plutôt la claire affirmation d’un changement radical de la sensibilité sociale et de la culture matérielle. Le naufrage n’est pas autre chose que le coût de la modernité d’une navigation-monde ibérique qui dominera la deuxième moitié du XVIe siècle.El libro 50 de la Historia natural y moral de las Indias de Gonzalo Fernández de Oviedo se presenta como la conclusión de treinta y cinco años de experiencia americana del autor, durante la cual acumuló relatos, testimonios, realizó entrevistas, como cronista al servicio de la corona. Este libro, poco estudiado, se suele definir como un texto híbrido de « literatura » en el que se mezcla todo tipo de naufragios (sucesos milagrosos, curiosos o espantosos a lo largo de 19 capítulos. Sin embargo presenta un interés singular para el historiador por la visión del mundo colonial que dibuja y la importancia concedida a la navegación, como experiencia compartida por la mayoría, en el proceso de construcción de la sociedad colonial. Este libro de los naufragios no es solo un panorama oscurecido de las Indias Occidentales, contrapuesto al discurso utópico, es más bien la clara afirmación de un cambio radical en la sensibilidad y en la cultura material. El naufragio no es sino el coste de la modernidad, de la navegación mundializada ibérica que domina los mares en el siglo XVI.

  19. Teaching Mathematical Proofs That Rely on Ideas from Physics.

    Science.gov (United States)

    Hanna, Gila; Jahnke, H. Niels; DeBruyn, Ysbrand; Lomas, Dennis

    2001-01-01

    Describes empirical research into the effectiveness of using concepts and principles of physics in the teaching of geometrical proofs. Demonstrates, tentatively, that proofs from physics could be a viable addition to the mathematics curriculum. (Author/MM)

  20. 依托国家级科研平台促进林业创新人才培养实践与思考%Practice and Consideration on Cultivation of Forestry Innovative Talents relying on State-level Scientiifc Research Platform

    Institute of Scientific and Technical Information of China (English)

    王君; 胡冬梅; 程武; 康向阳

    2015-01-01

    It is an effective approach to strengthen the cultivation of innovative talents relying on the existing high-level scientiifc research platform. The authors made some studies on the mode of undergraduate innovative talent cultivation relying on National Engineering Laboratory for Tree Breeding of Beijing Forestry University. A group of innovative elite students majored in biology and forestry were cultivated. Strengthening combination of classroom teaching and scientific research training, encouraging joint innovation crossing disciplines, and enhancing the sustainable culture have positive signiifcance for forestry innovative talent cultivation.%依托高等学校科研平台优势开展科研训练,是加强创新型人才培养的有效途径。基于北京林业大学林木育种国家工程实验室,对本科创新人才培养模式进行了一些有益的探索,培养了一批具有创新精神的生物学和林学专业优秀本科生,认为加强学研结合、鼓励跨学科联合创新、突出持续性培养,对于林业创新人才涌现具有积极意义。

  1. Result analysis of comprehensive nursing intervention on elderly patients at home with insomnia relying on sedative-hypnotic drugs for a long time%对居家长期使用镇静催眠药物的老年失眠患者综合护理干预结果分析

    Institute of Scientific and Technical Information of China (English)

    郝惠云

    2014-01-01

    目的:探讨综合护理干预对居家长期使用镇静催眠药物老年失眠患者的有效性。方法:对32例使用镇静催眠药物超过1年的老年失眠患者的睡眠和用药情况进行调查,并进行为期1年的综合护理干预。结果:老年失眠患者痊愈15例(46.87%),有效13例(40.63%),无效4例(12.5%)。镇静催眠药物服用人数明显减少,用药量下降。有药物依赖者11例(34.38%),其中7例(77.78%)在干预后消除了依赖。结论:综合护理干预,对居家长期使用镇静催眠药物的老年失眠患者,是必要的和有效的。%Objective:To discuss effectiveness of comprehensive nursing intervention on elderly patients at home with insomnia relying on sedative-hypnotic drugs for a long time. Methods:An investigation on sleeping state and drug usage had been done on 32 elderly patients with insomnia relying on sedative hypnotic drugs for over one year, and the comprehensive nursing intervention was car-ried on for more than one year. Results:15 cases (46. 87%) were cured, 13 cases (40. 63%) were effective, and 4 cases (12. 5%) were ineffective. The number of the patients using sedative-hypnotic drugs was significantly reduced, and the average drug usage was also declined. There were 11 drug-dependent cases (34. 38%); of which, 7 patients (77. 78%) succeeded in eliminating the de-pendence on drugs after the intervention. Conclusions:It is necessary and effective to have comprehensive nursing intervention on the elderly patients at home with insomnia relying on sedative hypnotic drugs for a long time.

  2. To Explore the Path of Sustainable Development in the Areas that Rely on Resources:A Case Study of Shenmu City in Shaanxi Province%资源依托区可持续发展的路径探索——以陕西省神木县为例

    Institute of Scientific and Technical Information of China (English)

    许仲举

    2011-01-01

    分析了神木县资源型经济的优势和劣势,提出了资源型经济转型的必要性和迫切性,进一步探讨了以优势产业为依托,统筹兼顾,协调区域经济社会发展思路和对策.%The advantages and disadvantages of resource-based economic of Shenmu county are analyzed, of the necessity and urgency of the transformation of resource-based economy are proposed, the ideas and strategies about relying on competitive industries, making overall plans and taking all factors into consideration, coordinating regional economic and social development are discussed.

  3. Akranların Sunduğu Sabit Bekleme Süreli Öğretim Gelişimsel Geriliği Olan Öğrencilere Tanıtıcı Levhaların Öğretiminde Etkili midir?

    Directory of Open Access Journals (Sweden)

    Serhat Yıldırım

    2002-07-01

    Full Text Available Araştırmada normal gelişim gösteren öğreten akranların sabit bekleme süreli öğretimi kullanarak gelişimsel geriliği olan öğrenen akranlarına tanıtıcı levhaları öğretmeleri değerlendirilmiştir. Araştırma iki amaç etrafında toplanmıştır: (a Öğreten akranlar gelişimsel geriliği olan Öğrenen akranlarına tanıtıcı levhaları öğretmek üzere, sabit bekleme süreli öğretimi güvenilir bicinule kullanabilirler mi? (b sabit bekleme süreli öğretim, öğrenen akranlara tanıtıcı levhaları öğretmekte etkili midir? Ayrıca, araştırmada akranlar tarafından sunulan sabit bekleme süreli öğretimin kişilerarası genelleme etkisi ve uygulama tamamlandıktan I, 3 ve 4 hafta sonra da kalıcılık etkisi incelenmiştir. Araştırmaya normal gelişim gösteren üç öğrenci öğreten akran, gelişimsel gerilik gösteren üç Öğrenci de öğrenen akran olarak katılmıştır. Tüm oturumlar birebir öğretim düzenlemesi ile gerçekleştirilmiştir. Araştırmada tek-denekli araştırma modellerinden yoklama evreli davranışlar arası çoklu yoklama modeli kullanılmış ve deneklerle replike edilmiştir. Araştırmada uygulama, yoklama, öğretim genelleme ve izleme oturumları düzenlenmiştir. Araştırma sonucunda. Öğreten akranların sabit bekleme süreli öğretimi güvenilir biçimde uyguladıkları görülmüştür. Gelişimsel geriliği olan öğrenen akranlar tanıtıcı levhaları etkili biçimde öğrenmişlerdir. Öğrenen akranlar tanıtıcı levhaları %I00 düzeyde genelleyebilmişlerdir. Ayrıca, öğrenen akranların tanıtıcı levhaların kalıcılığını yüksek düzeyde korudukları görülmüştür. The purpose of the present study was to investigate the effects of constant time delay procedure delivered by peer tutors on teaching expressively identifying community signs to the peer tutees with developmental disabilities. The study was twofold: (a Can peer tutors deliver constant

  4. Estimation of the impact of warfarin's time-in-therapeutic range on stroke and major bleeding rates and its influence on the medical cost avoidance associated with novel oral anticoagulant use-learnings from ARISTOTLE, ROCKET-AF, and RE-LY trials.

    Science.gov (United States)

    Amin, Alpesh; Deitelzweig, Steve; Jing, Yonghua; Makenbaeva, Dinara; Wiederkehr, Daniel; Lin, Jay; Graham, John

    2014-01-01

    Warfarin's time-in-therapeutic range (TTR) is highly variable among patients with nonvalvular atrial fibrillation (NVAF). The objective of this study was to estimate the impact of variations in wafarin's TTR on rates of stroke/systemic embolism (SSE) and major bleedings among NVAF patients in the ARISTOTLE, ROCKET-AF, and RE-LY trials. Additionally, differences in medical costs for clinical endpoints when novel oral anticoagulants (NOACs) were used instead of warfarin at different TTR values were estimated. Quartile ranges of TTR values and corresponding event rates (%/patient - year = %/py) of SSE and major bleedings among NVAF patients treated with warfarin were estimated from published literature and FDA documents. The associations of SSE and major bleeding rates with TTR values were evaluated by regression analysis and then the calculated regression coefficients were used in analysis of medical cost differences associated with use of each NOAC versus warfarin (2010 costs; US payer perspective) at different TTRs. Each 10 % increase in warfarin's TTR correlated with a -0.32%/py decrease in SSE rate (R(2) = 0.61; p estimated medical cost decreased from -$902 to -$83 for apixaban, from -$506 to +$314 for rivaroxaban, and from -$596 to +$223 for dabigatran. Among NVAF patients there is a significant negative correlation between warfarin's TTR and SSE rate, but not major bleedings. The variations in warfarin's TTR impacted the economic comparison of use of individual NOACs versus warfarin.

  5. 依托校园文化提高高职院校单招生自我管理能力调查研究%Investigation on Improving the Self-Management Ability of Independent Enrollment Students in Higher Vocational Colleges Relying on the Campus Culture

    Institute of Scientific and Technical Information of China (English)

    胡雪峰; 苏燕华; 李韵

    2014-01-01

    The work of independent enrollment students leads to many new problems in students management in higher vocational colleges. Improving self-management ability of independent enrollment students can make these problems solved efficiently. According to analyzing the characteristics of independent enrollment students, the author puts forward a series of measures to improve self-management ability of independent enrollment students relying on the campus culture, and provides a theoretical basis to cultivate the independent enrollment students.%单招生工作的开展,使高职院校学生管理出现了许多新问题,提高单招生自我管理能力能够使这些问题得到有效解决。本文通过分析单招生的特点,提出了依托校园文化提高单招生自我管理能力的系列措施,为单招生的培养提供了理论依据。

  6. Study on Present Development of Academic Research in Higher Vocational Colleges and Necessity of Relying on Scientific and Academic Research to Rejuvenate Higher Vocational Education%高职科研形势分析和实施“科研兴校”战略之必然

    Institute of Scientific and Technical Information of China (English)

    王进思

    2012-01-01

    The four major responsibilities of higher vocational education are training qualified workforce, undertaking scientific and academic research project, serving the social development and inheriting and passing on culture. To higher vocational colleges, the paper maintains that training qualified workforce is the core and the most important, while the scientific and academic research is the supporting force, serving social development is the ultimate objective, passing on culture is the fundamental responsibility. After studying the recent development of scientific and academic research in higher vocation colleges, the paper puts forward that the scientific and academic research is growing in importance, con- sidering which researchers and scholars should be motivated to devote themselves to the academic research. Finally, it elaborates on the necessity of relying on scientific and academic research to rejuvenate higher vocational education.%高职教育四大职能:"培养人才、科学研究、社会服务、文化传承",人才培养是核心,科学研究是支撑,社会服务是需要,文化传承是根本,科研工作的重要地位日益明显。为了提高高职教育工作者的科研积极性,文章分析了高职当前科研工作形势,阐述了实施"科研兴校"战略的必然要求。

  7. 元朝统治者信奉藏传佛教倚重萨迦派的原因探析%Reason Analysis of the Rulers of the Yuan Dynasty Believing in Tibetan Buddhism and Relying Heavily on the Sakyapa Sect

    Institute of Scientific and Technical Information of China (English)

    李文萍; 张付新

    2015-01-01

    The rulers the Yuan dynasty converted to Tibetan Buddhism and relied heavily on the Sakyapa sect to manage local affairs in Tibet, which became one of their management and governance policies in Tibet. An analy⁃sis has been done on the reasons of why the Yuan’s rulers executed the policy by referring to a large number of historical facts, and it is found that the rebellions of other Mongolian tribes in Central Asia and the close relation⁃ship between the Sakyapa and Mongolian royalty were the main reasons why the Yuan rulers adopted this policy.%元朝统治者选择信仰藏传佛教,并倚重萨迦派管理西藏地方事务,成为其管理和治理西藏的策略之一。文章基于大量的历史事实,分析了藏传佛教界对蒙古王室的积极传教,元朝中央为应对中亚蒙古宗王的反叛以及萨迦派与元王室的联姻等是影响元统治者采取这一政策的重要因素。

  8. From Permanent Neutrality to Relying on the United States--Japanese Government's National Security Conception in the Early Postwar Period%从永久中立到依附美国--简析战后初期日本政府的国家安全构想

    Institute of Scientific and Technical Information of China (English)

    刘树良

    2014-01-01

    In the early period of postwar, Japanese government worked hard to explore a way of how to defend Japan's national security from its demilitarization. Generally speaking, Japan's national security conception experienced an evolution process from permanent neutrality to relying on the United Nations, and ifnally to counting on the United States. Under the international environment of the conlfict between the United States and the Soviet Union, and facing the fact of occupancy by the United States, the policymakers of Japanese government made a strategic decision to depend on the United States by its “follow”mentality. Now the Japanese national security policy, which cultivated the conception of depending on the United States, is in an important transition period. It is noteworthy that how the Abe cabinet will execute its national security policy.%战后初期的日本政府从非武装化的现状出发,积极需求维护国家安全之路。概而言之,其国家安全构想经历了一个从永久中立国到依靠联合国,再到依附美国的演变过程。日本政府决策层最终选择依附美国,是在美苏对立的国际环境下,在被美国占领这一最大现实中,以“追随强者”的结盟心态做出的重大战略决择。在依附美国构想的基础上形成的战后日本国家安全政策目前正处于重要转型期,安倍内阁的国家安全政策走向特别值得关注。

  9. On the Significance andPoints of the Creation of Educational Environment in Kindergarten---Relying on Values Guidance Rather than Behavior Control%营造“峡谷式”幼儿教育环境的意义与要旨

    Institute of Scientific and Technical Information of China (English)

    田虎

    2016-01-01

    The creation of education environment in kindergarten should rely on the guidance of values rather than the control of behavior .Education environment of controlling type is to the disadvantage of developing children ’ s sense of security and freedom .Education environment of guide type can improve children ’ s independence ,creation and cooperation in educational activities ,and lay a solid foundation for the healthy growth of children .The creation of guide type education environment can satisfy the needs of developing children’s different concepts and behaviors ,ensure of children’s coordination ,and promote the development of children .%峡谷式教育环境的价值约束表现为指导性观点而非控制性行为。峡谷式教育环境能让幼儿既有确定感和可控制感,又有安全感和自由感。营造峡谷式教育环境,能够有效激发幼儿参与活动的主动性与创造性,保持幼儿活动的生成性,增强幼儿活动的合作性。营造峡谷式教育环境,需要革新教育思想,优化课程资源,改进师生关系,完善评价方式,增强幼儿观念和行动的自主性与协调性,帮助幼儿做最好的自己。

  10. Software developments in automated structure solution and crystallographic studies of the Sso10a2 and human C1 inhibitor protein

    NARCIS (Netherlands)

    Waterreus, Willem-Jan

    2013-01-01

    CRANK is a suite that links different macromolecular X-ray crystallographic programs to solve macromolecular crystal structures automatically from experimental phasing data. In chapter 2, several new algorithms implemented within CRANK increase the robustness and speed of the structure solution proc

  11. Analysis and Optimization of SSO Satellite Thermal Control Subsystem%太阳同步轨道卫星热控分系统分析及优化

    Institute of Scientific and Technical Information of China (English)

    吴文瑞; 黄海

    2012-01-01

    定义地心日照轨道坐标系,并在此坐标系下简化卫星与地球相对位置的复杂计算,以及卫星轨道外热流分析过程中相关角度的计算,使轨道外热流的分析仿真更加快速、简洁。以正六棱柱形卫星为例,建立热网络模型,对其表面在一圈轨道内所受的轨道外热流进行仿真,并结合仿真结果计算进出地影区时卫星内部的温度。在此基础上,建立以热控分系统多层隔热材料质量最小化为目标的优化问题;在满足高低温工况卫星内部温度在-10~+35℃范围内的约束下,对多层隔热材料厚度和散热窗大小进行了优化。%An Earth-original sunshine orbital coordinate system is defined.In this coordinate system,the complex computation of the relative position between satellite and the Earth can be bypassed,so the calculations of related angles in satellite orbital heat flux analysis become simple,and the satellite thermal analysis can be quickly accomplished.A thermal network model of a six-prism shaped satellite is built.The orbital heat flux on the surfaces of the satellite is simulated and applied in the model to obtain the satellite internal temperature at the start and end of eclipse period.Based on the model,an optimization of thermal control system to minimize multi-layer insulations mass in limitation of the satellite internal temperatures between-10℃ and +35℃ in both cold and hot cases is established.Multi-layer insulations thickness and radiator window area are optimized.

  12. 我国农村公共体育服务“以县为主”发展模式的困境与突破%Predicament and Breakthrough of the Development Model of "Relying Mainly on County" of the Public Sports Service in China's Rural Areas

    Institute of Scientific and Technical Information of China (English)

    郇昌店; 肖林鹏; 杨茜萍

    2012-01-01

    摘要:分税制以来,我国农村公共体育服务秉承“以县为主”的发展模式。这种发展模式体现了政府对公共服务职责的认定,但是在发展过程中面临一些困境:县级政府承担公共服务事权过多,体育事权在供给序列中较为滞后;大部制改革后,地方政府体育行政部门合并。供给能力有所降低;地方政府成悬浮型政府,制度内供给与制度外供给并存。并提出突破困境的建议:合理划分我国政府公共体育服务的事权,并逐渐法制化;建立以省市统筹为主、多级负责的财政投入体制;提高农村居民的决策能力.建立需求导向的供给体制。%The public sports service in China's rural areas has adopted the development model of "relying mainly on countyi" since the implementation of tax-sharing system. This kind of model shows government's acknowledgment of the responsibility of public service. Yet the development of the public sports service is facing some difficulties. The county governments take too much responsibility in public service. Sports responsibility is relatively lagging behind in the order of supply. After the administrative system reform, the merger of the sports administrative departments of the local government lowers the supply ability to some extent. The local governments have become suspended ones. The supplies inside and outside the system co- exist. The article suggests dividing rationally the Chinese government's power for public sports service and gradually realizing legalization, establishing a financial investment system of planning by the provinces or cities and directing by the different levels, improving rural residents' decision-making ability and forming a demand-oriented supply system.

  13. Analysis of EDS Fingerprint Spectra of Mineral Drug Montmorillonite Powder Relying on Dual Index Grade Sequence Individualized Pattern Recognition Method and their Quickly Quality Evaluation%矿物药蒙脱石散EDS指纹图谱双指标等级序列个性化模式识别分析及快速质量评价

    Institute of Scientific and Technical Information of China (English)

    邹华彬; 阿依古再丽·阿不力米提; 翟红

    2013-01-01

    EDS(energy dispersive spectrometer )element fingerprint spectra is able to quickly measure the kinds and the contents of elements in any mineral drug .In dual index grade sequence individualized pattern recognition method ,common (quantity) in-dex and variation (quantity) index ratios of any two samples’ fingerprint spectra are calculated ,and the individualized dual index sequence of each sample is constructed relying on its own EDS fingerprint spectra as the reference .Then the mean common (quantity ) index ratio P and the standard deviation S of all samples in each sample’s individualized dual index sequence are com-puted .On this basis ,for each sample ,its own similarity scale function P≥ P+ xS is built up .By this function ,the optimum x suitable for optimized classification/cluster of all samples is determined ,and the individualized characteristics sequence of one sample ,to which samples in the individualized characteristics sequence are significantly similar ,is decided also .Finally ,depen-ding on these individualized characteristics sequences ,the optimized classification/cluster of all samples can be carried out per-fectly without any prior knowledge related to them .This method is not only suitable for the quantitative analysis on fingerprint spectra being of only common peaks ,but also fits for that being of both common and variant peaks .In this study ,the EDS ele-ment fingerprint spectra of seven mineral drug montmorillonite powder samples from different companies were detected .Then common (quantity) index and variant (quantity) index ratios of peak area (or contents of majorly active element Fe ,Al ,Ca , Mg ,Si) among different EDS fingerprint spectra were obtained .In the similarity scale function P≥ P+ xS ,when x=0.5 ,these seven mineral drug montmorillonite powder samples could be quickly identified with high resolution , be classified into two groups ,and their quality could be evaluated precisely .In general EDS element fingerprint

  14. Feasibility of the Establishment of Community HyPertensive patients Self -management GrouP Relying on Social Net-work%依托社区高血压患者社会网络关系建立自我管理小组的可行性研究

    Institute of Scientific and Technical Information of China (English)

    廖娜; 张东; 段英伟; 王崴; 于国龙; 苏清香

    2014-01-01

    目的:了解社区内高血压患者之间的网络关系及特征,探讨高血压患者依托社会网络关系组建和扩展社区自我管理的可行性。方法2013年1-5月,采用多阶段随机抽样的方法抽取北京市西城区4个社区卫生服务站1074例高血压患者,采用问卷调查和测量的方法调查性别、年龄、血压等基本情况,采用提名生成法对社会网络度数中心度进行调查。使用构成比、度数中心度、Spearman等级相关等进行统计描述和分析。结果社区高血压患者社会网络度数中心度为0的占31.0%(333/1074),为1的占41.4%(445/1074),≥2的占27.6%(296/1074)。社会网络度数中心度为1的社区高血压患者中,64.5%(287/445)的患者选择家属为其社会网络关系对象;社会网络度数中心度≥2的社区高血压患者中,61.1%(181/296)的患者选择社区内病友为其社会网络关系对象。社区高血压患者社区活动组织参与率为8.7%(93/1074),社区高血压患者的社会网络度数中心度与参加社区活动组织数呈正相关关系(rs =0.521,P<0.05)。患者之间的小群体中存在中心人物。结论社区高血压患者之间社会网络特征较松散,社区活动组织内部的高血压患者之间网络关系较为密切,可作为社区高血压自我管理小组覆盖范围扩展的基础,社会网络分析有助于完成自我管理小组长的选择。%Objective To understand the social network and its characteristics among hypertensive patients in the com-munity and explore the feasibility of establishing and expanding self-management group relying on the social networking of the pa-tients. Methods 1 074 hypertensive patients were extracted from 4 community health service stations of Xicheng district with Multi-stage random sampling method. They were investigated about age,gender and blood pressure using questionnaires and physical examination. Degree

  15. Relying on Geographical Advantages of Red Culture and Promoting the Development of National Defense Education in Depth and Breadth to Explore the Ways of Developing Weinan Normal University's National Defense Education Activity%依托地域红色文化资源推动国防教育纵深发展--渭南师范学院国防教育活动途经探索

    Institute of Scientific and Technical Information of China (English)

    马耀斌

    2014-01-01

    面对国防教育活动形式单一,缺乏特色的现状,渭南师范学院结合学校所处的地域资源和地域文化优势,探索性地依托地域红色文化资源优势,通过对学生进行红色校史教育、革命圣地教育、“战地学风”宣讲等活动,有步骤地推动国防教育活动朝纵深发展,使国防教育引领学风校风,不断激发学生报效国家的自觉性和坚定性。%In the face of national defense education activities in the form of a single, lack of features, combined with the re-gional resources and geographical and cultural advantages in Weinan Normal University, the paper is exploratory to rely on the re-gional advantage of red cultural resources, by the red-school history education for students, the sacred place of the revolution of edu-cation, and the preaching activities of“the Battlefield Study Style”, step by step to promote the national defense education activities in depth development.

  16. Paper-Based Analytical Devices Relying on Visible-Light-Enhanced Glucose/Air Biofuel Cells.

    Science.gov (United States)

    Wu, Kaiqing; Zhang, Yan; Wang, Yanhu; Ge, Shenguang; Yan, Mei; Yu, Jinghua; Song, Xianrang

    2015-11-01

    A strategy that combines visible-light-enhanced biofuel cells (BFCs) and electrochemical immunosensor into paper-based analytical devices was proposed for sensitive detection of the carbohydrate antigen 15-3 (CA15-3). The gold nanoparticle modified paper electrode with large surface area and good conductibility was applied as an effective matrix for primary antibodies. The glucose dehydrogenase (GDH) modified gold-silver bimetallic nanoparticles were used as bioanodic biocatalyst and signal magnification label. Poly(terthiophene) (pTTh), a photoresponsive conducting polymer, served as catalyst in cathode for the reduction of oxygen upon illumination by visible light. In the bioanode, electrons were generated through the oxidation of glucose catalyzed by GDH. The amount of electrons is determined by the amount of GDH, which finally depended on the amount of CA15-3. In the cathode, electrons from the bioanode could combine with the generated holes in the HOMO energy level of cathode catalysts pTTh. Meanwhile, the high energy level photoexcited electrons were generated in the LUMO energy level and involved in the oxygen reduction reaction, finally resulting in an increasing current and a decreasing overpotential. According to the current signal, simple and efficient detection of CA15-3 was achieved.

  17. Mouse V1 population correlates of visual detection rely on heterogeneity within neuronal response patterns

    NARCIS (Netherlands)

    J.S. Montijn; P.M. Goltstein; C.M.A. Pennartz

    2015-01-01

    Previous studies have demonstrated the importance of the primary sensory cortex for the detection, discrimination and awareness of visual stimuli, but it is unknown how neuronal populations in this area process detected and undetected stimuli differently. Critical differences may reside in the mean

  18. Neuroprotective effects of 17β-estradiol rely on estrogen receptor membrane initiated signals

    Directory of Open Access Journals (Sweden)

    Marco eFiocchetti

    2012-04-01

    Full Text Available 17β-estradiol (E2 exerts protective effects in the central nervous system besides its crucial role in many physiological and pathological events. E2 effects are not restricted to the brain areas related with the control of the reproductive function, but rather are widespread throughout the developing and the adult brain. E2 actions are mediated by estrogen receptors (i.e., ERα and ERβ belonging to the nuclear receptor super family. As members of the ligand-regulated transcription factor family, the actions of ERs in the brain were thought to mediate only the E2 long-term transcriptional effects. However, a growing body of evidence has emerged indicating the presence of rapid, membrane initiated E2 effects in the brain which result independent from ER transcriptional activities and involved in E2-induced neuroprotection. Aim of this review is to focus on the rapid effects of E2 in the brain taking into account the specific contribution of the signaling pathway of ERβ subtype in neuroprotective actions of E2.

  19. Galileo IOV Electrical Power Subsystem Relies On Li-Ion Batter Charge Management Controlled By Hardware

    Science.gov (United States)

    Douay, N.

    2011-10-01

    In the frame of GALILEO In-Orbit Validation program which is composed of 4 satellites, Thales Alenia Space France has designed, developed and tested the Electrical Power Subsystem. Besides some classical design choices like: -50V regulated main power bus provided by the PCDU manufactured by Terma (DK), -Solar array, manufactured by Dutch-Space (NL), using Ga-As triple junction technology from Azur Space Power Solar GmbH, -SAFT (FR) Lithium-ion Battery for which cell package balancing function is required, -Solar Array Drive Mechanism, provided by RUAG Space Switzerland, to transfer the power. This subsystem features a fully autonomous, failure tolerant, battery charge management able to operate even after a complete unavailability of the on-board software. The battery charge management is implemented such that priority is always given to satisfy the satellite main bus needs in order to maintain the main bus regulation under MEA control. This battery charge management principle provides very high reliability and operational robustness. So, the paper describes : -the battery charge management concept using a combination of PCDU hardware and relevant battery lines monitoring, -the functional aspect of the single point failure free S4R (Sequential Switching Shunt Switch Regulator) and associated performances, -the failure modes isolated and passivated by this architecture. The paper will address as well the autonomous balancing function characteristics and performances.

  20. Polymerase gamma mutator mice rely on increased glycolytic flux for energy production.

    Science.gov (United States)

    Saleem, Ayesha; Safdar, Adeel; Kitaoka, Yu; Ma, Xiaoxing; Marquez, Olivia S; Akhtar, Mahmood; Nazli, Aisha; Suri, Rahul; Turnbull, John; Tarnopolsky, Mark A

    2015-03-01

    Several studies have illustrated that the polymerase gamma mutator (PolG) mice have reduced mitochondrial content secondary to systemic mitochondrial dysfunction, and subsequently a lower capacity to perform aerobic respiration and endurance exercise. We sought to delineate the extent of glycolysis as a means of energy production in the PolG mice in the absence of optimal mitochondrial function. PolG mice display an enhanced reliance on glycolysis as compared to their wild-type counterparts. This is evident by the resting hypoglycemia, higher PFK content, and elevated plasma lactate levels in the PolG mice. In vitro experiments provide further proof that PolG derived dermal fibroblasts have a higher rate of, and capacity for, glycolysis. PolG mice also have enhanced capacity to perform hepatic gluconeogenesis that is likely enhancing the Cori cycle capacity. Copyright © 2015 © Elsevier B.V. and Mitochondria Research Society. Published by Elsevier B.V. All rights reserved.

  1. Dépendre de la distribution d’aide alimentaire caritative / Relying on charity

    Directory of Open Access Journals (Sweden)

    Christine César

    2009-04-01

    Full Text Available Ce travail qualitatif de socio-anthropologie est centré sur l’analyse du récit de vie d’une famille de sans-papiers. L’objectif est de décrire et d’analyser de façon approfondie l’articulation entre l’aide alimentaire et les conditions de vie de personnes y ayant recours. Cette approche par l’alimentation apporte un éclairage particulier sur la situation des personnes se trouvant sans autorisation de travail sur le territoire, car les sans-papiers constituent une des figures extrêmes de dépendance au soutien mis en place par la société civile, les associations caritatives. Si la situation des sans-papiers se situe à la marge de la marge, elle révèle cependant des logiques de fonctionnement qui soulignent plus largement la dimension heuristique des pratiques alimentaires pour appréhender la variété des situations de pauvreté.Depending on charitable food aid distribution: the case of undocumented immigrants.This qualitative socio-anthropological work is focused on the analysis of a life narrative of an undocumented immigrant family. The objective of this paper is to describe and analyze in detail the relations between food aid and the living conditions of people dependent on charitable donations. This method of obtaining food provides a particular perspective on the situation of undocumented immigrants and reveals their extreme dependence on public programs, as seen by their submissive behavior when receiving aid from charitable associations. Analyzing the situation of undocumented immigrants who belong to the extreme margins of poverty reveals more than what influences their dietary practices: it helps to better understand the various situations of poverty.

  2. Improving listening comprehension skills relying on metacognitive strategies - focus on vocabulary and specific l2 instruction

    Directory of Open Access Journals (Sweden)

    Jerotijević-Tišma Danica

    2016-01-01

    Full Text Available The present paper aims at investigating the application of an instructional method specifically focused on the expansion of metacognitive awareness and its effect on Serbian EFL students’ listening comprehension. The current study is a follow-up research of a similar study by Vandergrift and Tafaghodtari (2010. However, we sought to expand the previous research by investigating the relationship between the students’ current level of L2 (target language vocabulary and listening test scores. Our study likewise differed in the sample of participants, the target language, teaching and testing material used, and the duration of the very experiment. To answer the proposed research questions we conducted an experiment with 57 Serbian secondary school EFL (English as a Foreign Language learners divided into experimental (n=27 and control group (n=30. The results of the pre- and post-tests of the two groups showed the beneficial effects of developing metacognitive strategies and the strong positive correlation between the level of vocabulary and listening comprehension. The paper underlines important pedagogical implications especially regarding the enhancement of metacognitive awareness and vocabulary proficiency of students in order to improve performance on listening comprehension tasks.

  3. Even simple forms of social learning rely on intention attribution in marmoset monkeys (Callithrix jacchus).

    Science.gov (United States)

    Burkart, Judith; Kupferberg, Aleksandra; Glasauer, Stefan; van Schaik, Carel

    2012-05-01

    Intention attribution guides the cognitively most demanding forms of social learning, such as imitation, thereby scaffolding cumulative cultural evolution. However, it is not thought to be necessary for more basic forms of social learning. Here we present evidence that in marmoset monkeys (Callithrix jacchus) even most basic forms of social learning such as enhancement depend on intention attribution. Marmosets perceived the behavior of a conspecific and a conspecific-like robot, but not that of a moving black box, as goal directed. Their subsequent choice behavior was shaped by social facilitation and stimulus enhancement, that is, by very simple forms of social learning, but only when exposed to the conspecific and robot, which they previously had perceived as intentional agents. We discuss the implications of this finding for contemporary debates about social learning, including emulation learning and ghost control studies, the necessity of goal-directed copying for cumulative cultural evolution, and the limits of current classification systems of social learning for the evolution of social and asocial learning.

  4. Cochrane and its prospects in Bosnia and Herzegovina: Relying on Cochrane Croatia

    Directory of Open Access Journals (Sweden)

    Mersiha Mahmić-Kaknjo

    2015-05-01

    Full Text Available In this article we describe Cochrane and its products: Cochrane systematic reviews (CSRs and other Cochrane evidence. Cochrane is a unique, international, non-profit organisation that offers health care providers, health care consumers and other decision makers unbiased and highly reliable information on health, which is pivotal for conscientious and responsible decision making in overall healthcare. Cochrane offers the highest ranked evidence in Evidence Based Medicine (EBM – systematic reviews. Currently, CSRs are freely available in BH, and therefore, they ought to be widely used, and understood. We will present the new Cochrane Strategy to 2020, which was the main topic of the 6th Croatian Cochrane Symposium (CroCoS, as well as explore prospects for spreading Cochrane activities to Bosnia and Herzegovina (BH, through collaboration with Cochrane Croatia. BH has no officially organized Cochrane activity, as yet. We hope that this article will raise awareness about Cochrane in BH, help promote its activities, and deepen the existing collaboration with Cochrane Croatia. There are already some changes being introduced concerning Cochrane – at least, in one half, the Federation of BH (FBH. Two documents symbolising official recognition of policy changes towards Cochrane have recently been published in the Official Gazette of FBH. Conclusion. Since founding a BH Cochrane Branch would be costly and difficult to achieve in a complicated environment, such as the one we have, BH could use the good will, experience, knowledge, and translated educational, training and web materials of Cochrane Croatia, particularly given the language similarities, to promote evidence based medicine in BH.

  5. We rely more on mobiles and portable devices but remember fewer things

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    According to a survey released on Friday,the boom in mobiles and portable devices that store reams of personal information has created a generation incapable of memorizing simple things.A quarter of those polled said they couldn’t remember their landline number,while two-thirds couldn’recall the birthdays of more than three friends or family members.

  6. Stage-specific functions of Semaphorin7A during adult hippocampal neurogenesis rely on distinct receptors.

    Science.gov (United States)

    Jongbloets, Bart C; Lemstra, Suzanne; Schellino, Roberta; Broekhoven, Mark H; Parkash, Jyoti; Hellemons, Anita J C G M; Mao, Tianyi; Giacobini, Paolo; van Praag, Henriette; De Marchis, Silvia; Ramakers, Geert M J; Pasterkamp, R Jeroen

    2017-03-10

    The guidance protein Semaphorin7A (Sema7A) is required for the proper development of the immune and nervous systems. Despite strong expression in the mature brain, the role of Sema7A in the adult remains poorly defined. Here we show that Sema7A utilizes different cell surface receptors to control the proliferation and differentiation of neural progenitors in the adult hippocampal dentate gyrus (DG), one of the select regions of the mature brain where neurogenesis occurs. PlexinC1 is selectively expressed in early neural progenitors in the adult mouse DG and mediates the inhibitory effects of Sema7A on progenitor proliferation. Subsequently, during differentiation of adult-born DG granule cells, Sema7A promotes dendrite growth, complexity and spine development through β1-subunit-containing integrin receptors. Our data identify Sema7A as a key regulator of adult hippocampal neurogenesis, providing an example of how differential receptor usage spatiotemporally controls and diversifies the effects of guidance cues in the adult brain.

  7. The chronic pain conundrum: should we CHANGE from relying on past history to assessing prognostic factors?

    Science.gov (United States)

    Pergolizzi, Joseph; Ahlbeck, Karsten; Aldington, Dominic; Alon, Eli; Collett, Beverly; Coluzzi, Flaminia; Huygen, Frank; Jaksch, Wolfgang; Kocot-Kępska, Magdalena; Mangas, Ana Cristina; Margarit, Cesar; Mavrocordatos, Philippe; Morlion, Bart; Müller-Schwefe, Gerhard; Nicolaou, Andrew; Pérez Hernández, Concepción; Sichere, Patrick; Varrassi, Giustino

    2012-02-01

    Despite limited empirical support, chronic pain has traditionally been defined mainly on the basis of its duration, which takes no account of the causative mechanisms or its clinical significance. For this commentary on current pain management practice, the CHANGE PAIN Advisory Board considered the evidence for adopting a prognostic definition of chronic pain. The rationale underlying this approach is to take psychological and behavioural factors into account, as well as the multidimensional nature of pain. Measures of pain intensity, interference with everyday activities, role disability, depression, duration and number of pain sites are used to calculate a risk score, which indicates the likelihood of a patient having pain in the future. The consistency of a prognostic definition with the concept of integrated patient care was also considered. When this method was compared with the number of pain days experienced over the previous 6 months--in patients with back pain, headache or orofacial pain--it was a better predictor of clinically significant pain 6 months later for all three pain conditions. Further evidence supporting this approach is that several factors other than the duration of pain have been shown to be important prognostic indicators, including unemployment, functional disability, anxiety and self-rated health. The use of a multifactorial risk score may also suggest specific measures to improve outcomes, such as addressing emotional distress. These measures should be undertaken as part of an integrated pain management strategy; chronic pain is a biopsychosocial phenomenon and all aspects of the patient's pain must be dealt with appropriately and simultaneously for treatment to be effective. The implementation of a prognostic definition and wider adoption of integrated care could bring significant advantages. However, these measures require improved training in pain management and structural revision of specialist facilities, for which political support is essential.

  8. CERN birthplace of the World Wide Web relies on Day's Communiqué

    CERN Multimedia

    2001-01-01

    In close collaboration with CERN, Day developed a platform to enable interested research groups to integrate disparate data sources and to author content based on individual needs... Days Virtual Repository - The ContentBus - allows the integration of data sources and provides for the presentation of all work processes at CERN, from pure text to complex research data. With data procured in real time from the original location, it avoids data redundancy, insufficient scalability and unneccessary allocation of resources for constant data reconcilliation and additional database licenses.

  9. Detecting Bias in Meta-Analyses of Distance Education Research: Big Pictures We Can Rely On

    Science.gov (United States)

    Bernard, Robert M.; Borokhovski, Eugene; Tamim, Rana M.

    2014-01-01

    This article has two interrelated purposes. The first is to explain how various forms of bias, if introduced during any stage of a meta-analysis, can provide the consumer with a misimpression of the state of a research literature. Five of the most important bias-producing aspects of a meta-analysis are presented and discussed. Second, armed with…

  10. Viroids: from genotype to phenotype just relying on RNA sequence and structural motifs

    Directory of Open Access Journals (Sweden)

    Ricardo eFlores

    2012-06-01

    Full Text Available As a consequence of two unique physical properties, small size and circularity, viroid RNAs do not code for proteins and thus depend on RNA sequence/structural motifs for interacting with host proteins that mediate their invasion, replication, spread, and circumvention of defensive barriers. Viroid genomes fold up on themselves adopting collapsed secondary structures wherein stretches of nucleotides stabilized by Watson-Crick pairs are flanked by apparently unstructured loops. However, compelling data show that they are instead stabilized by alternative non-canonical pairs and that specific loops in the rod-like secondary structure, characteristic of Potato spindle tuber viroid and most other members of the family Pospiviroidae, are critical for replication and systemic trafficking. In contrast, rather than folding into a rod-like secondary structure, most members of the family Avsunvioidae adopt multibranched conformations occasionally stabilized by kissing loop interactions critical for viroid viability in vivo. Besides these most stable secondary structures, viroid RNAs alternatively adopt during replication transient metastable conformations containing elements of local higher-order structure, prominent among which are the hammerhead ribozymes catalyzing a key replicative step in the family Avsunvioidae, and certain conserved hairpins that also mediate replication steps in the family Pospiviroidae. Therefore, different RNA structures ⎯either global or local ⎯ determine different functions, thus highlighting the need for in-depth structural studies on viroid RNAs.

  11. An Esterification Kinetics Experiment that Relies on the Sense of Smell

    Science.gov (United States)

    Bromfield-Lee, Deborah C.; Oliver-Hoyo, Maria T.

    2009-01-01

    This experiment involves an esterification synthesis to study reaction kinetics where students explore these topics utilizing the sense of smell rather than the traditional approach of using spectroscopic methods. Students study the effects of various factors including the concentration of the carboxylic acid and the amounts of the catalyst or…

  12. Nature does not rely on long-lived electronic quantum coherence for photosynthetic energy transfer

    CERN Document Server

    Duan, Hong-Guang; Cogdell, Richard; Ashraf, Khuram; Stevens, Amy L; Thorwart, Michael; Miller, R J Dwayne

    2016-01-01

    During the first steps of photosynthesis, the energy of impinging solar photons is transformed into electronic excitation energy of the light-harvesting biomolecular complexes. The subsequent energy transfer to the reaction center is understood in terms of exciton quasiparticles which move on a grid of biomolecular sites on typical time scales less than 100 femtoseconds (fs). Since the early days of quantum mechanics, this energy transfer is described as an incoherent Forster hopping with classical site occupation probabilities, but with quantum mechanically determined rate constants. This orthodox picture has been challenged by ultrafast optical spectroscopy experiments with the Fenna-Matthews-Olson protein in which interference oscillatory signals up to 1.5 picoseconds were reported and interpreted as direct evidence of exceptionally long-lived electronic quantum coherence. Here, we show that the optical 2D photon echo spectra of this complex at ambient temperature in aqueous solution do not provide evidenc...

  13. 76 FR 69545 - Conditions and Requirements for Relying on Component Part Testing or Certification, or Another...

    Science.gov (United States)

    2011-11-08

    ... supply chain to include the manufacturers of the subcomponents used in component parts. (Response 6... design or manufacturing process, including the sourcing of component parts. The final rule on ``Testing... finished product on the finished product certifier. Another commenter liked the strong chain of custody and...

  14. Breast cancer stem cells rely on fermentative glycolysis and are sensitive to 2-deoxyglucose treatment.

    Science.gov (United States)

    Ciavardelli, D; Rossi, C; Barcaroli, D; Volpe, S; Consalvo, A; Zucchelli, M; De Cola, A; Scavo, E; Carollo, R; D'Agostino, D; Forlì, F; D'Aguanno, S; Todaro, M; Stassi, G; Di Ilio, C; De Laurenzi, V; Urbani, A

    2014-07-17

    A number of studies suggest that cancer stem cells are essential for tumour growth, and failure to target these cells can result in tumour relapse. As this population of cells has been shown to be resistant to radiation and chemotherapy, it is essential to understand their biology and identify new therapeutic approaches. Targeting cancer metabolism is a potential alternative strategy to counteract tumour growth and recurrence. Here we applied a proteomic and targeted metabolomic analysis in order to point out the main metabolic differences between breast cancer cells grown as spheres and thus enriched in cancer stem cells were compared with the same cells grown in adherent differentiating conditions. This integrated approach allowed us to identify a metabolic phenotype associated with the stem-like condition and shows that breast cancer stem cells (BCSCs) shift from mitochondrial oxidative phosphorylation towards fermentative glycolysis. Functional validation of proteomic and metabolic data provide evidences for increased activities of key enzymes of anaerobic glucose fate such as pyruvate kinase M2 isoform, lactate dehydrogenase and glucose 6-phopshate dehydrogenase in cancer stem cells as well as different redox status. Moreover, we show that treatment with 2-deoxyglucose, a well known inhibitor of glycolysis, inhibits BCSC proliferation when used alone and shows a synergic effect when used in combination with doxorubicin. In conclusion, we suggest that inhibition of glycolysis may be a potentially effective strategy to target BCSCs.

  15. Usefulness and dangers of relying on grant acknowledgments in an observatory bibliography

    Science.gov (United States)

    Winkelman, Sherry; Rots, Arnold

    2016-07-01

    The purpose of this paper is to present a quantitative assessment of how well grant and/or program acknowledgments reflect the science impact of Chandra observing, archive, and theory programs and to assess whether observatory acknowledgments alone are a good indicator for inclusion in an observatory bibliography. For grant citations we find that curators will often need to determine the correct grant being cited and they will need to assess relationship between the content of a paper and the grant proposal being cited for statistics to be meaningful. We also find a significant number of papers can be attributed to observing programs through grant links only and that performing full-text searches against the ADS for grant numbers can lead to additional articles for inclusion in the bibliography. When looking at acknowledgment sections as a whole, we find that using an observatory acknowledgment as the sole source for determining inclusion in a bibliography will greatly underestimate the number of science papers attributable to the observatory.

  16. Damage-induced DNA replication stalling relies on MAPK-activated protein kinase 2 activity

    DEFF Research Database (Denmark)

    Köpper, Frederik; Bierwirth, Cathrin; Schön, Margarete

    2013-01-01

    DNA damage can obstruct replication forks, resulting in replicative stress. By siRNA screening, we identified kinases involved in the accumulation of phosphohistone 2AX (γH2AX) upon UV irradiation-induced replication stress. Surprisingly, the strongest reduction of phosphohistone 2AX followed...... replication impaired by gemcitabine or by Chk1 inhibition. This rescue strictly depended on translesion DNA polymerases. In conclusion, instead of being an unavoidable consequence of DNA damage, alterations of replication speed and origin firing depend on MK2-mediated signaling....... knockdown of the MAP kinase-activated protein kinase 2 (MK2), a kinase currently implicated in p38 stress signaling and G2 arrest. Depletion or inhibition of MK2 also protected cells from DNA damage-induced cell death, and mice deficient for MK2 displayed decreased apoptosis in the skin upon UV irradiation...

  17. Brucella Intracellular Life Relies on the Transmembrane Protein CD98 Heavy Chain.

    Science.gov (United States)

    Keriel, Anne; Botella, Eric; Estrach, Soline; Bragagnolo, Gabriel; Vergunst, Annette C; Feral, Chloe C; O'Callaghan, David

    2015-06-01

    Brucella are intracellular bacterial pathogens that use a type IV secretion system (T4SS) to escape host defenses and create a niche in which they can multiply. Although the importance of Brucella T4SS is clear, little is known about its interactions with host cell structures. In this study, we identified the eukaryotic protein CD98hc as a partner for Brucella T4SS subunit VirB2. This transmembrane glycoprotein is involved in amino acid transport, modulation of integrin signaling, and cell-to-cell fusion. Knockdown of CD98hc expression in HeLa cells demonstrated that it is essential for Brucella infection. Using knockout dermal fibroblasts, we confirmed its role for Brucella but found that it is not required for Salmonella infection. CD98hc transiently accumulates around the bacteria during the early phases of infection and is required for both optimal bacterial uptake and intracellular multiplication of Brucella. These results provide new insights into the complex interplay between Brucella and its host.

  18. Toward a Better Nutritional Aiding in Disasters: Relying on Lessons Learned during the Bam Earthquake.

    Science.gov (United States)

    Nekouie Moghadam, Mahmoud; Amiresmaieli, Mohammadreza; Hassibi, Mohammad; Doostan, Farideh; Khosravi, Sajad

    2017-03-27

    Introduction Examining various problems in the aftermath of disasters is very important to the disaster victims. Managing and coordinating food supply and its distribution among the victims is one of the most important problems after an earthquake. Therefore, the purpose of this study was to recognize problems and experiences in the field of nutritional aiding during an earthquake.

  19. Self-awareness in neurodegenerative disease relies on neural structures mediating reward-driven attention

    Science.gov (United States)

    Shany-Ur, Tal; Lin, Nancy; Rosen, Howard J.; Sollberger, Marc; Miller, Bruce L.

    2014-01-01

    Accurate self-awareness is essential for adapting one’s tasks and goals to one’s actual abilities. Patients with neurodegenerative diseases, particularly those with right frontal involvement, often present with poor self-awareness of their functional limitations that may exacerbate their already jeopardized decision-making and behaviour. We studied the structural neuroanatomical basis for impaired self-awareness among patients with neurodegenerative disease and healthy older adults. One hundred and twenty-four participants (78 patients with neurodegenerative diseases including Alzheimer’s disease, behavioural variant frontotemporal dementia, right-temporal frontotemporal dementia, semantic variant and non-fluent variant primary progressive aphasia, and 46 healthy controls) described themselves on the Patient Competency Rating Scale, rating observable functioning across four domains (daily living activities, cognitive, emotional control, interpersonal). All participants underwent structural magnetic resonance imaging. Informants also described subjects’ functioning on the same scale. Self-awareness was measured by comparing self and informant ratings. Group differences in discrepancy scores were analysed using general linear models, controlling for age, sex and disease severity. Compared with controls, patients with behavioural variant frontotemporal dementia overestimated their functioning in all domains, patients with Alzheimer’s disease overestimated cognitive and emotional functioning, patients with right-temporal frontotemporal dementia overestimated interpersonal functioning, and patients with non-fluent aphasia overestimated emotional and interpersonal functioning. Patients with semantic variant aphasia did not overestimate functioning on any domain. To examine the neuroanatomic correlates of impaired self-awareness, discrepancy scores were correlated with brain volume using voxel-based morphometry. To identify the unique neural correlates of overlooking versus exaggerating deficits, overestimation and underestimation scores were analysed separately, controlling for age, sex, total intracranial volume and extent of actual functional decline. Atrophy related to overestimating one’s functioning included bilateral, right greater than left frontal and subcortical regions, including dorsal superior and middle frontal gyri, lateral and medial orbitofrontal gyri, right anterior insula, putamen, thalamus, and caudate, and midbrain and pons. Thus, our patients’ tendency to under-represent their functional decline was related to degeneration of domain-general dorsal frontal regions involved in attention, as well as orbitofrontal and subcortical regions likely involved in assigning a reward value to self-related processing and maintaining accurate self-knowledge. The anatomic correlates of underestimation (right rostral anterior cingulate cortex, uncorrected significance level) were distinct from overestimation and had a substantially smaller effect size. This suggests that underestimation or ‘tarnishing’ may be influenced by non-structural neurobiological and sociocultural factors, and should not be considered to be on a continuum with overestimation or ‘polishing’ of functional capacity, which appears to be more directly mediated by neural circuit dysfunction. PMID:24951639

  20. Increasing Uncertainty: The Dangers of Relying on Conventional Forces for Nuclear Deterrence

    Science.gov (United States)

    2016-03-14

    threat of assured retaliation to convince an enemy not to attack. Some people have the misconception that deterrence did not come into existence until...posture. Foreign Perspective The security environment has changed dramatically in the five years since the NPR’s publication —but not for the better, as...a long tradition in the former Soviet Union and continues in mod- ern Russia. Prior to the US-led “reset” in US-Russia relations in 2009, Russian

  1. Building Traversing NAT IPv6 Tunnel Gateway System Relies on Netfilter/Iptable Framework

    Institute of Scientific and Technical Information of China (English)

    YU Yong; WANG wei; HAN He-qin; SUN wei

    2006-01-01

    The transition from IPv4 to IPv6 is doomed to be a long process. The network Address translation (NAT) technology is used very popularly in IPv4 network to make up the shortage of network address. It is a desiderated problem to make the users behind NAT gateway to access to IPv6 networks. By studying the transition technology from IPv4 to IPv6 and introducing NAT technology in IPv6, a scenario is put forward through 6to4 tunnel The scenario is implemented and the gateway system's performance is analyzed.

  2. Enhancing memory performance after organic brain disease relies on retrieval processes rather than encoding or consolidation.

    Science.gov (United States)

    Hildebrandt, Helmut; Gehrmann, Annika; Modden, Claudia; Eling, Paul

    2011-02-01

    Neuropsychological rehabilitation of memory performance is still a controversial topic, and rehabilitation studies have not analyzed to which stage of memory processing (encoding, consolidation, or retrieval) enhancement may be attributed. We first examined the efficacy of a computer training program for stroke patients, based on a previous study (Hildebrandt, Clausing, Janssen, & Modden, 2007a) for memory-impaired patients of a rehabilitation unit and compared it with the standard group treatment. In a second randomized controlled experiment, we trained two groups of 15 patients with mild to moderate memory disorders, caused by organic brain lesions, with the same two treatment approaches. We used several standard tests to analyze improvement of memory functions, focusing on separate parameters for encoding, consolidation, and retrieval. We developed for that purpose a new word-list learning test, which allowed assessment of response to novelty and a systematic comparison of free recall after learning of semantically structured and nonstructured word lists. The first treatment experiment showed significant improvement of verbal learning for patients treated with the computer software program. The second experiment showed that memory improvement was based exclusively on retrieval processes, whereas no specific change was found for encoding and consolidation. However, the two groups of the second experiment showed no significant differences for the treatment, although the absolute scores pointed in the same direction as in the first experiment.

  3. Taian Companion Machinery Co.,Ltd:Seize Opportunities Relying on Self-Intellectual Innovatio

    Institute of Scientific and Technical Information of China (English)

    Sherry Liu

    2011-01-01

    Since its first participation of ShanghaiTex 2011 last year,Companion Machinery Co.,Ltd hasmade an important step,"letting theworld know Companion Machinery,and making Companion Machinery go outside" by upholding concepts of "Being friends, partners and collaborators".

  4. Relying on Your Own Best Judgment: Imputing Values to Missing Information in Decision Making.

    Science.gov (United States)

    Johnson, Richard D.; And Others

    Processes involved in making estimates of the value of missing information that could help in a decision making process were studied. Hypothetical purchases of ground beef were selected for the study as such purchases have the desirable property of quantifying both the price and quality. A total of 150 students at the University of Iowa rated the…

  5. Defense Headquarters: Geographic Combatant Commands Rely on Subordinate Commands for Mission Management and Execution

    Science.gov (United States)

    2016-06-30

    Additionally, in November 2015, the Secretary of Defense directed a review of DOD’s organizational structures and is considering a number of actions... changes have occurred since the 2011 Unified Command Plan was published. Page 2 GAO-16-652R Defense Headquarters sufficiently reliable for the...commands. • We found that the combatant commands utilize subordinate commands to execute assigned missions. The organizational construct DOD created

  6. Direct conversion of root primordium into shoot meristem relies on timing of stem cell niche development.

    Science.gov (United States)

    Rosspopoff, Olga; Chelysheva, Liudmila; Saffar, Julie; Lecorgne, Lena; Gey, Delphine; Caillieux, Erwann; Colot, Vincent; Roudier, François; Hilson, Pierre; Berthomé, Richard; Da Costa, Marco; Rech, Philippe

    2017-04-01

    To understand how the identity of an organ can be switched, we studied the transformation of lateral root primordia (LRP) into shoot meristems in Arabidopsis root segments. In this system, the cytokinin-induced conversion does not involve the formation of callus-like structures. Detailed analysis showed that the conversion sequence starts with a mitotic pause and is concomitant with the differential expression of regulators of root and shoot development. The conversion requires the presence of apical stem cells, and only LRP at stages VI or VII can be switched. It is engaged as soon as cell divisions resume because their position and orientation differ in the converting organ compared with the undisturbed emerging LRP. By alternating auxin and cytokinin treatments, we showed that the root and shoot organogenetic programs are remarkably plastic, as the status of the same plant stem cell niche can be reversed repeatedly within a set developmental window. Thus, the networks at play in the meristem of a root can morph in the span of a couple of cell division cycles into those of a shoot, and back, through transdifferentiation. © 2017. Published by The Company of Biologists Ltd.

  7. Spider orb webs rely on radial threads to absorb prey kinetic energy.

    Science.gov (United States)

    Sensenig, Andrew T; Lorentz, Kimberly A; Kelly, Sean P; Blackledge, Todd A

    2012-08-07

    The kinetic energy of flying insect prey is a formidable challenge for orb-weaving spiders. These spiders construct two-dimensional, round webs from a combination of stiff, strong radial silk and highly elastic, glue-coated capture spirals. Orb webs must first stop the flight of insect prey and then retain those insects long enough to be subdued by the spiders. Consequently, spider silks rank among the toughest known biomaterials. The large number of silk threads composing a web suggests that aerodynamic dissipation may also play an important role in stopping prey. Here, we quantify energy dissipation in orb webs spun by diverse species of spiders using data derived from high-speed videos of web deformation under prey impact. By integrating video data with material testing of silks, we compare the relative contributions of radial silk, the capture spiral and aerodynamic dissipation. Radial silk dominated energy absorption in all webs, with the potential to account for approximately 100 per cent of the work of stopping prey in larger webs. The most generous estimates for the roles of capture spirals and aerodynamic dissipation show that they rarely contribute more than 30 per cent and 10 per cent of the total work of stopping prey, respectively, and then only for smaller orb webs. The reliance of spider orb webs upon internal energy absorption by radial threads for prey capture suggests that the material properties of the capture spirals are largely unconstrained by the selective pressures of stopping prey and can instead evolve freely in response to alternative functional constraints such as adhering to prey.

  8. Something To Rely On : The Influence of Stable and Fleeting Drivers on Moral Behavior

    NARCIS (Netherlands)

    G.G. van Houwelingen (Gijs)

    2015-01-01

    markdownabstract__Abstract__ In virtually any situation we are bound to encounter short-lived influences that lure us to act in a certain way. The influence of such ‘fleeting drivers’ may or may not be in line our long-term goals and commitments (‘stable drivers’). Moral behaviour in particular oft

  9. Desiccation tolerance in the tardigrade Richtersius coronifer relies on muscle mediated structural reorganization.

    Science.gov (United States)

    Halberg, Kenneth Agerlin; Jørgensen, Aslak; Møbjerg, Nadja

    2013-01-01

    Life unfolds within a framework of constraining abiotic factors, yet some organisms are adapted to handle large fluctuations in physical and chemical parameters. Tardigrades are microscopic ecdysozoans well known for their ability to endure hostile conditions, such as complete desiccation--a phenomenon called anhydrobiosis. During dehydration, anhydrobiotic animals undergo a series of anatomical changes. Whether this reorganization is an essential regulated event mediated by active controlled processes, or merely a passive result of the dehydration process, has not been clearly determined. Here, we investigate parameters pivotal to the formation of the so-called "tun", a state that in tardigrades and rotifers marks the entrance into anhydrobiosis. Estimation of body volume in the eutardigrade Richtersius coronifer reveals an 87 % reduction in volume from the hydrated active state to the dehydrated tun state, underlining the structural stress associated with entering anhydrobiosis. Survival experiments with pharmacological inhibitors of mitochondrial energy production and muscle contractions show that i) mitochondrial energy production is a prerequisite for surviving desiccation, ii) uncoupling the mitochondria abolishes tun formation, and iii) inhibiting the musculature impairs the ability to form viable tuns. We moreover provide a comparative analysis of the structural changes involved in tun formation, using a combination of cytochemistry, confocal laser scanning microscopy and 3D reconstructions as well as scanning electron microscopy. Our data reveal that the musculature mediates a structural reorganization vital for anhydrobiotic survival, and furthermore that maintaining structural integrity is essential for resumption of life following rehydration.

  10. Desiccation tolerance in the tardigrade Richtersius coronifer relies on muscle mediated structural reorganization.

    Directory of Open Access Journals (Sweden)

    Kenneth Agerlin Halberg

    Full Text Available Life unfolds within a framework of constraining abiotic factors, yet some organisms are adapted to handle large fluctuations in physical and chemical parameters. Tardigrades are microscopic ecdysozoans well known for their ability to endure hostile conditions, such as complete desiccation--a phenomenon called anhydrobiosis. During dehydration, anhydrobiotic animals undergo a series of anatomical changes. Whether this reorganization is an essential regulated event mediated by active controlled processes, or merely a passive result of the dehydration process, has not been clearly determined. Here, we investigate parameters pivotal to the formation of the so-called "tun", a state that in tardigrades and rotifers marks the entrance into anhydrobiosis. Estimation of body volume in the eutardigrade Richtersius coronifer reveals an 87 % reduction in volume from the hydrated active state to the dehydrated tun state, underlining the structural stress associated with entering anhydrobiosis. Survival experiments with pharmacological inhibitors of mitochondrial energy production and muscle contractions show that i mitochondrial energy production is a prerequisite for surviving desiccation, ii uncoupling the mitochondria abolishes tun formation, and iii inhibiting the musculature impairs the ability to form viable tuns. We moreover provide a comparative analysis of the structural changes involved in tun formation, using a combination of cytochemistry, confocal laser scanning microscopy and 3D reconstructions as well as scanning electron microscopy. Our data reveal that the musculature mediates a structural reorganization vital for anhydrobiotic survival, and furthermore that maintaining structural integrity is essential for resumption of life following rehydration.

  11. Processing of Neutrophil α-Defensins Does Not Rely on Serine Proteases In Vivo

    DEFF Research Database (Denmark)

    Glenthøj, Andreas; Nickles, Katrin; Cowland, Jack;

    2015-01-01

    in promyelocytes: Neutrophil elastase (NE), cathepsin G (CG), and proteinase 3 (PR3), all of which are able to process recombinant proHNP into HNP in vitro. We investigated whether serine proteases are in fact responsible for processing of proHNP in human bone marrow cells and in human and murine myeloid cell...... lines. Subcellular fractionation of the human promyelocytic cell line PLB-985 demonstrated proHNP processing to commence in fractions containing endoplasmic reticulum. Processing of 35S-proHNP was insensitive to serine protease inhibitors. Simultaneous knockdown of NE, CG, and PR3 did not decrease pro...

  12. Enabling Eco-Friendly Choices by Relying on the Proportional-Thinking Heuristic

    Directory of Open Access Journals (Sweden)

    Cleotilde Gonzalez

    2013-01-01

    Full Text Available Ecological (eco taxes are promising mechanisms to enable eco-friendly decisions, but few people prefer them. In this study, we present a way in which eco-tax options may be communicated to general public to encourage their payment. Our implementation (called “information presentation” takes advantage of the non-linear relationship between eco-tax payments and CO2 emissions and the human reliance on the proportional-thinking heuristic. According to the proportional-thinking heuristic, people are likely to prefer a small eco-tax increase and judge larger eco-tax increases to cause proportionally greater CO2 emissions reductions. In an online study, participants were asked to choose between eco-tax increases in two problems: In one, a smaller eco-tax increase resulted in greater CO2 emissions reduction, while in the other, a smaller tax increase resulted in lesser CO2 emissions reduction. Although the larger eco-tax increase did not reduce CO2 emissions the most, across both problems, people judged larger eco-tax increases to cause proportionally greater reductions in CO2 emissions and preferred smaller tax increases. Thus, eco-tax policies would benefit by presenting information in terms of eco-tax increases, such that smaller eco-tax increases (which are more attractive and are likely to be chosen by people cause greater CO2 emissions reductions.

  13. Self-awareness in neurodegenerative disease relies on neural structures mediating reward-driven attention.

    Science.gov (United States)

    Shany-Ur, Tal; Lin, Nancy; Rosen, Howard J; Sollberger, Marc; Miller, Bruce L; Rankin, Katherine P

    2014-08-01

    Accurate self-awareness is essential for adapting one's tasks and goals to one's actual abilities. Patients with neurodegenerative diseases, particularly those with right frontal involvement, often present with poor self-awareness of their functional limitations that may exacerbate their already jeopardized decision-making and behaviour. We studied the structural neuroanatomical basis for impaired self-awareness among patients with neurodegenerative disease and healthy older adults. One hundred and twenty-four participants (78 patients with neurodegenerative diseases including Alzheimer's disease, behavioural variant frontotemporal dementia, right-temporal frontotemporal dementia, semantic variant and non-fluent variant primary progressive aphasia, and 46 healthy controls) described themselves on the Patient Competency Rating Scale, rating observable functioning across four domains (daily living activities, cognitive, emotional control, interpersonal). All participants underwent structural magnetic resonance imaging. Informants also described subjects' functioning on the same scale. Self-awareness was measured by comparing self and informant ratings. Group differences in discrepancy scores were analysed using general linear models, controlling for age, sex and disease severity. Compared with controls, patients with behavioural variant frontotemporal dementia overestimated their functioning in all domains, patients with Alzheimer's disease overestimated cognitive and emotional functioning, patients with right-temporal frontotemporal dementia overestimated interpersonal functioning, and patients with non-fluent aphasia overestimated emotional and interpersonal functioning. Patients with semantic variant aphasia did not overestimate functioning on any domain. To examine the neuroanatomic correlates of impaired self-awareness, discrepancy scores were correlated with brain volume using voxel-based morphometry. To identify the unique neural correlates of overlooking versus exaggerating deficits, overestimation and underestimation scores were analysed separately, controlling for age, sex, total intracranial volume and extent of actual functional decline. Atrophy related to overestimating one's functioning included bilateral, right greater than left frontal and subcortical regions, including dorsal superior and middle frontal gyri, lateral and medial orbitofrontal gyri, right anterior insula, putamen, thalamus, and caudate, and midbrain and pons. Thus, our patients' tendency to under-represent their functional decline was related to degeneration of domain-general dorsal frontal regions involved in attention, as well as orbitofrontal and subcortical regions likely involved in assigning a reward value to self-related processing and maintaining accurate self-knowledge. The anatomic correlates of underestimation (right rostral anterior cingulate cortex, uncorrected significance level) were distinct from overestimation and had a substantially smaller effect size. This suggests that underestimation or 'tarnishing' may be influenced by non-structural neurobiological and sociocultural factors, and should not be considered to be on a continuum with overestimation or 'polishing' of functional capacity, which appears to be more directly mediated by neural circuit dysfunction.

  14. A tumour suppressor network relying on the polyamine-hypusine axis.

    Science.gov (United States)

    Scuoppo, Claudio; Miething, Cornelius; Lindqvist, Lisa; Reyes, José; Ruse, Cristian; Appelmann, Iris; Yoon, Seungtai; Krasnitz, Alexander; Teruya-Feldstein, Julie; Pappin, Darryl; Pelletier, Jerry; Lowe, Scott W

    2012-07-12

    Tumour suppressor genes encode a broad class of molecules whose mutational attenuation contributes to malignant progression. In the canonical situation, the tumour suppressor is completely inactivated through a two-hit process involving a point mutation in one allele and chromosomal deletion of the other. Here, to identify tumour suppressor genes in lymphoma, we screen a short hairpin RNA library targeting genes deleted in human lymphomas. We functionally identify those genes whose suppression promotes tumorigenesis in a mouse lymphoma model. Of the nine tumour suppressors we identified, eight correspond to genes occurring in three physically linked 'clusters', suggesting that the common occurrence of large chromosomal deletions in human tumours reflects selective pressure to attenuate multiple genes. Among the new tumour suppressors are adenosylmethionine decarboxylase 1 (AMD1) and eukaryotic translation initiation factor 5A (eIF5A), two genes associated with hypusine, a unique amino acid produced as a product of polyamine metabolism through a highly conserved pathway. Through a secondary screen surveying the impact of all polyamine enzymes on tumorigenesis, we establish the polyamine-hypusine axis as a new tumour suppressor network regulating apoptosis. Unexpectedly, heterozygous deletions encompassing AMD1 and eIF5A often occur together in human lymphomas and co-suppression of both genes promotes lymphomagenesis in mice. Thus, some tumour suppressor functions can be disabled through a two-step process targeting different genes acting in the same pathway.

  15. Efficient peroxydisulfate activation process not relying on sulfate radical generation for water pollutant degradation

    KAUST Repository

    Zhang, Tao

    2014-05-20

    Peroxydisulfate (PDS) is an appealing oxidant for contaminated groundwater and toxic industrial wastewaters. Activation of PDS is necessary for application because of its low reactivity. Present activation processes always generate sulfate radicals as actual oxidants which unselectively oxidize organics and halide anions reducing oxidation capacity of PDS and producing toxic halogenated products. Here we report that copper oxide (CuO) can efficiently activate PDS under mild conditions without producing sulfate radicals. The PDS/CuO coupled process is most efficient at neutral pH for decomposing a model compound, 2,4-dichlorophenol (2,4-DCP). In a continuous-flow reaction with an empty-bed contact time of 0.55 min, over 90% of 2,4-DCP (initially 20 μM) and 90% of adsorbable organic chlorine (AOCl) can be removed at the PDS/2,4-DCP molar ratio of 1 and 4, respectively. Based on kinetic study and surface characterization, PDS is proposed to be first activated by CuO through outer-sphere interaction, the rate-limiting step, followed by a rapid reaction with 2,4-DCP present in the solution. In the presence of ubiquitous chloride ions in groundwater/industrial wastewater, the PDS/CuO oxidation shows significant advantages over sulfate radical oxidation by achieving much higher 2,4-DCP degradation capacity and avoiding the formation of highly chlorinated degradation products. This work provides a new way of PDS activation for contaminant removal. © 2014 American Chemical Society.

  16. Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.

    Science.gov (United States)

    Ulbricht, Anna; Eppler, Felix J; Tapia, Victor E; van der Ven, Peter F M; Hampe, Nico; Hersch, Nils; Vakeel, Padmanabhan; Stadel, Daniela; Haas, Albert; Saftig, Paul; Behrends, Christian; Fürst, Dieter O; Volkmer, Rudolf; Hoffmann, Bernd; Kolanus, Waldemar; Höhfeld, Jörg

    2013-03-04

    Mechanical tension is an ever-present physiological stimulus essential for the development and homeostasis of locomotory, cardiovascular, respiratory, and urogenital systems. Tension sensing contributes to stem cell differentiation, immune cell recruitment, and tumorigenesis. Yet, how mechanical signals are transduced inside cells remains poorly understood. Here, we identify chaperone-assisted selective autophagy (CASA) as a tension-induced autophagy pathway essential for mechanotransduction in muscle and immune cells. The CASA complex, comprised of the molecular chaperones Hsc70 and HspB8 and the cochaperone BAG3, senses the mechanical unfolding of the actin-crosslinking protein filamin. Together with the chaperone-associated ubiquitin ligase CHIP, the complex initiates the ubiquitin-dependent autophagic sorting of damaged filamin to lysosomes for degradation. Autophagosome formation during CASA depends on an interaction of BAG3 with synaptopodin-2 (SYNPO2). This interaction is mediated by the BAG3 WW domain and facilitates cooperation with an autophagosome membrane fusion complex. BAG3 also utilizes its WW domain to engage in YAP/TAZ signaling. Via this pathway, BAG3 stimulates filamin transcription to maintain actin anchoring and crosslinking under mechanical tension. By integrating tension sensing, autophagosome formation, and transcription regulation during mechanotransduction, the CASA machinery ensures tissue homeostasis and regulates fundamental cellular processes such as adhesion, migration, and proliferation. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. Arsenate tolerance in Silene paradoxa does not rely on phytochelatin-dependent sequestration.

    Science.gov (United States)

    Arnetoli, Miluscia; Vooijs, Riet; ten Bookum, Wilma; Galardi, Francesca; Gonnelli, Cristina; Gabbrielli, Roberto; Schat, Henk; Verkleij, Jos A C

    2008-04-01

    Arsenate tolerance, As accumulation and As-induced phytochelatin accumulation were compared in populations of Silene paradoxa, one from a mine site enriched in As, Cu and Zn, the other from an uncontaminated site. The mine population was significantly more arsenate-tolerant. Arsenate uptake and root-to-shoot transport were slightly but significantly higher in the non-mine plants. The difference in uptake was quantitatively insufficient to explain the difference in tolerance between the populations. As accumulation in the roots was similar in both populations, but the mine plants accumulated much less phytochelatins than the non-mine plants. The mean phytochelatin chain length, however, was higher in the mine population, possibly due to a constitutively lower cellular glutathione level. It is argued that the mine plants must possess an arsenic detoxification mechanism other than arsenate reduction and subsequent phytochelatin-based sequestration. This alternative mechanism might explain at least some part of the superior tolerance in the mine plants.

  18. A tumour suppressor network relying on the polyamine–hypusine axis

    Science.gov (United States)

    Scuoppo, Claudio; Miething, Cornelius; Lindqvist, Lisa; Reyes, José; Ruse, Cristian; Appelmann, Iris; Yoon, Seungtai; Krasnitz, Alexander; Teruya-Feldstein, Julie; Pappin, Darryl; Pelletier, Jerry; Lowe, Scott W.

    2012-01-01

    Tumour suppressor genes encode a broad class of molecules whose mutational attenuation contributes to malignant progression. In the canonical situation, the tumour suppressor is completely inactivated through a two-hit process involving a point mutation in one allele and chromosomal deletion of the other1. Here, to identify tumour suppressor genes in lymphoma, we screen a short hairpin RNA library targeting genes deleted in human lymphomas. We functionally identify those genes whose suppression promotes tumorigenesis in a mouse lymphoma model. Of the nine tumour suppressors we identified, eight correspond to genes occurring in three physically linked ‘clusters’, suggesting that the common occurrence of large chromosomal deletions in human tumours reflects selective pressure to attenuate multiple genes. Among the new tumour suppressors are adenosylmethionine decarboxylase 1 (AMD1) and eukaryotic translation initiation factor 5A (eIF5A), two genes associated with hypusine, a unique amino acid produced as a product of polyamine metabolism through a highly conserved pathway2. Through a secondary screen surveying the impact of all polyamine enzymes on tumorigenesis, we establish the polyamine–hypusine axis as a new tumour suppressor network regulating apoptosis. Unexpectedly, heterozygous deletions encompassing AMD1 and eIF5A often occur together in human lymphomas and co-suppression of both genes promotes lymphomagenesis in mice. Thus, some tumour suppressor functions can be disabled through a two-step process targeting different genes acting in the same pathway. PMID:22722845

  19. Radiation of the Nod-independent Aeschynomene relies on multiple allopolyploid speciation events.

    Science.gov (United States)

    Arrighi, Jean-François; Chaintreuil, Clémence; Cartieaux, Fabienne; Cardi, C; Rodier-Goud, M; Brown, Spencer C; Boursot, Marc; D'Hont, Angélique; Dreyfus, Bernard; Giraud, Eric

    2014-03-01

    • The semi-aquatic legumes belonging to the genus Aeschynomene constitute a premium system for investigating the origin and evolution of unusual symbiotic features such as stem nodulation and the presence of a Nod-independent infection process. This latter apparently arose in a single Aeschynomene lineage. But how this unique Nod-independent group then radiated is not yet known. • We have investigated the role of polyploidy in Aeschynomene speciation via a case study of the pantropical A. indica and then extended the analysis to the other Nod-independent species. For this, we combined SSR genotyping, genome characterization through flow cytometry, chromosome counting, FISH and GISH experiments, molecular phylogenies using ITS and single nuclear gene sequences, and artificial hybridizations. • These analyses demonstrate the existence of an A. indica polyploid species complex comprising A. evenia (C. Wright) (2n = 2x = 20), A. indica L. s.s. (2n = 4x = 40) and a new hexaploid form (2n = 6x = 60). This latter contains the two genomes present in the tetraploid (A. evenia and A. scabra) and another unidentified genome. Two other species, A. pratensis and A. virginica, are also shown to be of allopolyploid origin. • This work reveals multiple hybridization/polyploidization events, thus highlighting a prominent role of allopolyploidy in the radiation of the Nod-independent Aeschynomene.

  20. A Distinguisher-Based Attack of a Homomorphic Encryption Scheme Relying on Reed-Solomon Codes

    CERN Document Server

    Gauthier, Valérie; Tillich, Jean-Pierre

    2012-01-01

    Bogdanov and Lee suggested a homomorphic public-key encryption scheme based on error correcting codes. The underlying public code is a modified Reed-Solomon code obtained from inserting a zero submatrix in the Vandermonde generating matrix defining it. The columns that define this submatrix are kept secret and form a set $L$. We give here a distinguisher that detects if one or several columns belong to $L$ or not. This distinguisher is obtained by considering the code generated by component-wise products of codewords of the public code (the so called "square code"). This operation is applied to punctured versions of this square code obtained by picking a subset $I$ of the whole set of columns. It turns out that the dimension of the punctured square code is directly related to the cardinality of the intersection of $I$ with $L$. This allows an attack which recovers the full set $L$ and which can then decrypt any ciphertext.

  1. Chromatin determinants of the inner-centromere rely on replication factors with functions that impart cohesion.

    Science.gov (United States)

    Abe, Takuya; Kawasumi, Ryotaro; Arakawa, Hiroshi; Hori, Tetsuya; Shirahige, Katsuhiko; Losada, Ana; Fukagawa, Tatsuo; Branzei, Dana

    2016-10-18

    Replication fork-associated factors promote genome integrity and protect against cancer. Mutations in the DDX11 helicase and the ESCO2 acetyltransferase also cause related developmental disorders classified as cohesinopathies. Here we generated vertebrate model cell lines of these disorders and cohesinopathies-related genes. We found that vertebrate DDX11 and Tim-Tipin are individually needed to compensate for ESCO2 loss in chromosome segregation, with DDX11 also playing complementary roles with ESCO2 in centromeric cohesion. Our study reveals that overt centromeric cohesion loss does not necessarily precede chromosome missegregation, while both these problems correlate with, and possibly originate from, inner-centromere defects involving reduced phosphorylation of histone H3T3 (pH3T3) in the region. Interestingly, the mitotic pH3T3 mark was defective in all analyzed replication-related mutants with functions in cohesion. The results pinpoint mitotic pH3T3 as a postreplicative chromatin mark that is sensitive to replication stress and conducts with different kinetics to robust centromeric cohesion and correct chromosome segregation.

  2. Gustatory Habituation in "Drosophila" Relies on "Rutabaga" (Adenylate Cyclase)-Dependent Plasticity of GABAergic Inhibitory Neurons

    Science.gov (United States)

    Paranjpe, Pushkar; Rodrigues, Veronica; VijayRaghavan, K.; Ramaswami, Mani

    2012-01-01

    In some situations, animals seem to ignore stimuli which in other contexts elicit a robust response. This attenuation in behavior, which enables animals to ignore a familiar, unreinforced stimulus, is called habituation. Despite the ubiquity of this phenomenon, it is generally poorly understood in terms of the underlying neural circuitry. Hungry…

  3. DIGITAL BOOKS RELY ON SOCIAL READING TO FIND NEW SUCCESS PROPOSALS AND FORMATS

    Directory of Open Access Journals (Sweden)

    Berta García Orosa

    2016-07-01

    Full Text Available Digital reading development has reached turning point. In view of the recent data, the edition of electronic books has dropped for the second consecutive year in 2014, while paper books production has increased (MECD, 2015. The last report from the Fundación Telefónica (2015 points out that six out ten Spanish people prefer printed books instead of the digital ones. This article presents the symptoms of a possible change of tendency in digital books. There is a need for a new digital ecosystem of digital texts production and reception. Its major focus should be the social reading, understood as a space for reception, interpretation, and creation.

  4. Hantavirus infection mimicking leptospirosis: how long are we going to rely on clinical suspicion?

    Science.gov (United States)

    Dahanayaka, Niroshana Jathum; Agampodi, Suneth Buddhika; Bandaranayaka, Anoma Kumari; Priyankara, Sumudu; Vinetz, Joshep M

    2014-08-13

    Hantavirus infections and leptospirosis can have similar clinical and epidemiological features. We present here a case study of a young farmer with fever during the post-flood leptospirosis outbreak in Anuradhapura, Sri Lanka, in 2011. He presented with a classical clinical picture of leptospirosis and was managed and notified as a case of leptospirosis. Retrospective analysis of a stored serum sample confirmed acute hantavirus infection. Diagnosis of newly identified or emerging infectious diseases such as hantavirus infection is challenging due to the lack of diagnostic facilities in developing countries. This case highlights the need for improving diagnostic facilities, educating medical staff, and conducting population-based prospective studies on hantavirus infections in Sri Lanka.

  5. Why auditors increasingly rely on analytical procedures : an empirical approach in Portugal

    OpenAIRE

    Pinho, Carlos; Santos, Paula; Martins, António Eduardo

    2013-01-01

    Congresso realizado em Lisboa de 10-11 de outubro de 2013 The increasing sophistication and quantity of transactions considered to be financial facts included in accounting records and financial statements raises additional issues in terms of financial auditing. Finding more corroborating evidence for the claims made in balances, transaction classes and disclosures is naturally made more difficult by the fact that accounting information and financial reports are increasingly complex bo...

  6. Monkeys Rely on Recency of Stimulus Repetition When Solving Short-Term Memory Tasks

    Science.gov (United States)

    Wittig, John H., Jr.; Richmond, Barry J.

    2014-01-01

    Seven monkeys performed variants of two short-term memory tasks that others have used to differentiate between selective and nonselective memory mechanisms. The first task was to view a list of sequentially presented images and identify whether a test matched any image from the list, but not a distractor from a preceding list. Performance was best…

  7. Monkeys Rely on Recency of Stimulus Repetition When Solving Short-Term Memory Tasks

    Science.gov (United States)

    Wittig, John H., Jr.; Richmond, Barry J.

    2014-01-01

    Seven monkeys performed variants of two short-term memory tasks that others have used to differentiate between selective and nonselective memory mechanisms. The first task was to view a list of sequentially presented images and identify whether a test matched any image from the list, but not a distractor from a preceding list. Performance was best…

  8. Polymodal Responses in C. elegans Phasmid Neurons Rely on Multiple Intracellular and Intercellular Signaling Pathways

    Science.gov (United States)

    Zou, Wenjuan; Cheng, Hankui; Li, Shitian; Yue, Xiaomin; Xue, Yadan; Chen, Sixi; Kang, Lijun

    2017-01-01

    Animals utilize specialized sensory neurons enabling the detection of a wide range of environmental stimuli from the presence of toxic chemicals to that of touch. However, how these neurons discriminate between different kinds of stimuli remains poorly understood. By combining in vivo calcium imaging and molecular genetic manipulation, here we investigate the response patterns and the underlying mechanisms of the C. elegans phasmid neurons PHA/PHB to a variety of sensory stimuli. Our observations demonstrate that PHA/PHB neurons are polymodal sensory neurons which sense harmful chemicals, hyperosmotic solutions and mechanical stimulation. A repulsive concentration of IAA induces calcium elevations in PHA/PHB and both OSM-9 and TAX-4 are essential for IAA-sensing in PHA/PHB. Nevertheless, the PHA/PHB neurons are inhibited by copper and post-synaptically activated by copper removal. Neuropeptide is likely involved in copper removal-induced calcium elevations in PHA/PHB. Furthermore, mechanical stimulation activates PHA/PHB in an OSM-9-dependent manner. Our work demonstrates how PHA/PHB neurons respond to multiple environmental stimuli and lays a foundation for the further understanding of the mechanisms of polymodal signaling, such as nociception, in more complex organisms. PMID:28195191

  9. Motor learning and cross-limb transfer rely upon distinct neural adaptation processes.

    Science.gov (United States)

    Stöckel, Tino; Carroll, Timothy J; Summers, Jeffery J; Hinder, Mark R

    2016-08-01

    Performance benefits conferred in the untrained limb after unilateral motor practice are termed cross-limb transfer. Although the effect is robust, the neural mechanisms remain incompletely understood. In this study we used noninvasive brain stimulation to reveal that the neural adaptations that mediate motor learning in the trained limb are distinct from those that underlie cross-limb transfer to the opposite limb. Thirty-six participants practiced a ballistic motor task with their right index finger (150 trials), followed by intermittent theta-burst stimulation (iTBS) applied to the trained (contralateral) primary motor cortex (cM1 group), the untrained (ipsilateral) M1 (iM1 group), or the vertex (sham group). After stimulation, another 150 training trials were undertaken. Motor performance and corticospinal excitability were assessed before motor training, pre- and post-iTBS, and after the second training bout. For all groups, training significantly increased performance and excitability of the trained hand, and performance, but not excitability, of the untrained hand, indicating transfer at the level of task performance. The typical facilitatory effect of iTBS on MEPs was reversed for cM1, suggesting homeostatic metaplasticity, and prior performance gains in the trained hand were degraded, suggesting that iTBS interfered with learning. In stark contrast, iM1 iTBS facilitated both performance and excitability for the untrained hand. Importantly, the effects of cM1 and iM1 iTBS on behavior were exclusive to the hand contralateral to stimulation, suggesting that adaptations within the untrained M1 contribute to cross-limb transfer. However, the neural processes that mediate learning in the trained hemisphere vs. transfer in the untrained hemisphere appear distinct.

  10. Something To Rely On : The Influence of Stable and Fleeting Drivers on Moral Behavior

    NARCIS (Netherlands)

    G.G. van Houwelingen (Gijs)

    2015-01-01

    markdownabstract__Abstract__ In virtually any situation we are bound to encounter short-lived influences that lure us to act in a certain way. The influence of such ‘fleeting drivers’ may or may not be in line our long-term goals and commitments (‘stable drivers’). Moral behaviour in particular oft

  11. Cognitive agents - a procedural perspective relying on the predictability of Object-Action-Complexes (OACs)

    DEFF Research Database (Denmark)

    Wörgötter, Florentin; Agostino, A.; Krüger, Norbert

    2009-01-01

    Embodied cognition suggests that complex cognitive traits can only arise when agents have a body situated in the world. The aspects of embodiment and situatedness are being discussed here from the perspective of linear systems theory. This perspective treats bodies as dynamic, temporally variable...... entities, which can be extended (or curtailed) at their boundaries. We show how acting agents can, for example, actively extend their body for some time by incorporating predictably behaving parts of the world and how this affects the transfer functions. We suggest that primates have mastered...... and behaviour. Hence, in this article we have tried to show how predictability can be used to augment the agent's body and to acquire knowledge about the external world, possibly leading to more advanced cognitive traits....

  12. Arsenate tolerance in Silene paradoxa does not rely on phytochelatin-dependent sequestration

    Energy Technology Data Exchange (ETDEWEB)

    Arnetoli, Miluscia [Section of Plant Ecology and Physiology, Department of Plant Biology, University of Florence, via Micheli 1, 50121 Firenze (Italy)], E-mail: miluscia@gmail.com; Vooijs, Riet; Bookum, Wilma ten [Institute of Molecular and Cellular Biology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam (Netherlands); Galardi, Francesca; Gonnelli, Cristina; Gabbrielli, Roberto [Section of Plant Ecology and Physiology, Department of Plant Biology, University of Florence, via Micheli 1, 50121 Firenze (Italy); Schat, Henk; Verkleij, Jos A.C. [Institute of Molecular and Cellular Biology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam (Netherlands)

    2008-04-15

    Arsenate tolerance, As accumulation and As-induced phytochelatin accumulation were compared in populations of Silene paradoxa, one from a mine site enriched in As, Cu and Zn, the other from an uncontaminated site. The mine population was significantly more arsenate-tolerant. Arsenate uptake and root-to-shoot transport were slightly but significantly higher in the non-mine plants. The difference in uptake was quantitatively insufficient to explain the difference in tolerance between the populations. As accumulation in the roots was similar in both populations, but the mine plants accumulated much less phytochelatins than the non-mine plants. The mean phytochelatin chain length, however, was higher in the mine population, possibly due to a constitutively lower cellular glutathione level. It is argued that the mine plants must possess an arsenic detoxification mechanism other than arsenate reduction and subsequent phytochelatin-based sequestration. This alternative mechanism might explain at least some part of the superior tolerance in the mine plants. - Neither decreased uptake nor phytochelatins seem to play a role in the As tolerance in Silene paradoxa.

  13. The Low Pitch of High-Frequency Complex Tones Relies on Temporal Fine Structure Information

    DEFF Research Database (Denmark)

    Santurette, Sébastien; Dau, Torsten

    2010-01-01

    structure (TFS) cues has been a matter of debate. It is also controversial up to which frequency TFS information remains available, and to what extent envelope cues become dominant as frequency increases. Using a pitch-matching paradigm, this study investigated whether the pitch of transposed tones...

  14. Intra-ER sorting of the peroxisomal membrane protein Pex3 relies on its luminal domain

    Directory of Open Access Journals (Sweden)

    Mohammad H. Fakieh

    2013-06-01

    Pex3 is an evolutionarily conserved type III peroxisomal membrane protein required for peroxisome formation. It is inserted into the ER membrane and sorted via an ER subdomain (the peroxisomal ER, or pER to peroxisomes. By constructing chimeras between Pex3 and the type III ER membrane protein Sec66, we have been able to separate the signals that mediate insertion of Pex3 into the ER from those that mediate sorting within the ER to the pER subdomain. The N-terminal 17-amino acid segment of Pex3 contains two signals that are each sufficient for sorting to the pER: a chimeric protein containing the N-terminal domain of Pex3 fused to the transmembrane and cytoplasmic segments of Sec66 sorts to the pER in wild type cells, and does not colocalise with peroxisomes. Subsequent transport to existing peroxisomes requires the Pex3 transmembrane segment. When expressed in Drosophila S2R+ cells, ScPex3 targeting to peroxisomes is dependent on the intra-ER sorting signals in the N-terminal segment. The N-terminal segments of both human and Drosophila Pex3 contain intra-ER sorting information and can replace that of ScPex3. Our analysis has uncovered the signals within Pex3 required for the various steps of its transport to peroxisomes. Our generation of versions of Pex3 that are blocked at each stage along its transport pathway provides a tool to dissect the mechanism, as well as the molecular machinery required at each step of the pathway.

  15. Persistent cell migration and adhesion rely on retrograde transport of β(1) integrin.

    Science.gov (United States)

    Shafaq-Zadah, Massiullah; Gomes-Santos, Carina S; Bardin, Sabine; Maiuri, Paolo; Maurin, Mathieu; Iranzo, Julian; Gautreau, Alexis; Lamaze, Christophe; Caswell, Patrick; Goud, Bruno; Johannes, Ludger

    2016-01-01

    Integrins have key functions in cell adhesion and migration. How integrins are dynamically relocalized to the leading edge in highly polarized migratory cells has remained unexplored. Here, we demonstrate that β1 integrin (known as PAT-3 in Caenorhabditis elegans), but not β3, is transported from the plasma membrane to the trans-Golgi network, to be resecreted in a polarized manner. This retrograde trafficking is restricted to the non-ligand-bound conformation of β1 integrin. Retrograde trafficking inhibition abrogates several β1-integrin-specific functions such as cell adhesion in early embryonic development of mice, and persistent cell migration in the developing posterior gonad arm of C. elegans. Our results establish a paradigm according to which retrograde trafficking, and not endosomal recycling, is the key driver for β1 integrin function in highly polarized cells. These data more generally suggest that the retrograde route is used to relocalize plasma membrane machinery from previous sites of function to the leading edge of migratory cells.

  16. The achievement gap: Should we rely on SAT scores to tell us anything about it?

    Directory of Open Access Journals (Sweden)

    Dale Whittington

    2004-04-01

    Full Text Available Increasing numbers of students taking the SAT have declined to identify their race/ethnicity. I examined the impact of non-respondents on the validity of reported racial/ethnic differences and year-to-year changes in test performance. Using an analysis reported by Wainer (1988 and SAT data from 1996 to 2003, I confirmed Wainer’s findings that non-respondents prevent accurate estimations of group differences based on SAT data. I then explored the impact of College Board press release information on news reports about the achievement gap. I found frequent reports of racial/ethnic differences in SAT scores and year-to-year changes in scores but negligible consideration of non-respondents. Press releases and media reports should include information about non-respondents and their impact on accuracy of reported differences based on race/ethnicity.

  17. To what extent does organic farming rely on nutrient inflows from conventional farming?

    Science.gov (United States)

    Nowak, Benjamin; Nesme, Thomas; David, Christophe; Pellerin, Sylvain

    2013-12-01

    Organic farming is increasingly recognized as a prototype for sustainable agriculture. Its guidelines ban the use of artificial fertilizers. However, organic farms may import nutrients from conventional farming through material exchanges. In this study, we aimed at estimating the magnitude of these flows through the quantification of nitrogen, phosphorus and potassium inflows from conventional farming to organic farming. Material inflows and outflows were collected for two cropping years on 63 farms. The farms were located in three French agricultural districts distributed over a gradient of farming activity defined by both the stocking rate and the ratio of the farm area under arable crops. Our results showed that on average, inflows from conventional farming were 23%, 73% and 53% for nitrogen, phosphorus and potassium, respectively. These inflows were strongly determined by the farm production systems. However, for farms similar in terms of production systems, the inflows also depended on the local context, such as the proximity of organic livestock farms: the reliance of organic farming on conventional farming was lower in mixed than in specialized districts. These results highlight the necessity to quantify the contribution of nutrient inflows from conventional farming when assessing organic farming and development scenarios.

  18. The Florida Harvester Ant, Pogonomyrmex badius, Relies on Germination to Consume Large Seeds.

    Science.gov (United States)

    Tschinkel, Walter R; Kwapich, Christina L

    2016-01-01

    The Florida harvester ant, Pogonomyrmex badius, is one of many ant species and genera that stores large numbers of seeds in damp, underground chambers for later consumption. A comparison of the sizes of seeds recovered from storage chambers with those of seed husks discarded following consumption revealed that the used seeds are far smaller than stored seeds. This difference in use-rate was confirmed in field and laboratory colonies by offering marked seeds of various sizes and monitoring the appearance of size-specific chaff. Because foragers collect a range of seed sizes but only open small seeds, large seeds accumulate, forming 70% or more of the weight of seed stores. Major workers increase the rates at which small and medium seeds are opened, but do not increase the size range of opened seeds. Experiments limiting ant access to portions of natural seed chambers showed that seeds germinate during storage, but that the ants rapidly remove them. When offered alongside non germinating seeds, germinating seeds were preferentially fed to larvae. The rate of germination during the annual cycle was determined by both burial in artificial chambers at various depths and under four laboratory temperatures. The germination rate depends upon the species of seed, the soil/laboratory temperature and/or the elapsed time. The seasonal soil temperature cycle generated germination patterns that vary with the mix of locally-available seeds. Taken together, exploitation of germination greatly increases the resources available to the ants in space and time. While the largest seeds may have the nutritional value of 15 small seeds, the inability of workers to open large seeds at will precludes them from rapid use during catastrophic events. The harvester ant's approach to seed harvesting is therefore two-pronged, with both immediate and delayed payoffs arising from the tendency to forage for a wide variety of seeds sizes.

  19. A College That Relied on NeXT Computers Plans To Switch to Apple.

    Science.gov (United States)

    Wilson, David L.

    1997-01-01

    Allegheny College (Pennsylvania), which uses NeXT computers, was dismayed when the technically superior operating system was orphaned but are now delighted that the company has been bought by Apple Computer and will make the operating system standard on Apple computers. The object-oriented operating system allows relatively unsophisticated users…

  20. Short-term Internet search using makes people rely on search engines when facing unknown issues.

    Science.gov (United States)

    Wang, Yifan; Wu, Lingdan; Luo, Liang; Zhang, Yifen; Dong, Guangheng

    2017-01-01

    The Internet search engines, which have powerful search/sort functions and ease of use features, have become an indispensable tool for many individuals. The current study is to test whether the short-term Internet search training can make people more dependent on it. Thirty-one subjects out of forty subjects completed the search training study which included a pre-test, a six-day's training of Internet search, and a post-test. During the pre- and post- tests, subjects were asked to search online the answers to 40 unusual questions, remember the answers and recall them in the scanner. Un-learned questions were randomly presented at the recalling stage in order to elicited search impulse. Comparing to the pre-test, subjects in the post-test reported higher impulse to use search engines to answer un-learned questions. Consistently, subjects showed higher brain activations in dorsolateral prefrontal cortex and anterior cingulate cortex in the post-test than in the pre-test. In addition, there were significant positive correlations self-reported search impulse and brain responses in the frontal areas. The results suggest that a simple six-day's Internet search training can make people dependent on the search tools when facing unknown issues. People are easily dependent on the Internet search engines.

  1. Investigating the Acquisition of Software Systems that Rely on Open Architecture and Open Source Software

    Science.gov (United States)

    2010-03-01

    Copyright (c) 1996-2008, Daniel Stenberg <daniel@haxx.se>. 16. PostgreSQL Database Management System 17. FreeType. Copyright (c) 2007 The FreeType...may not be open for access, review, modification, or possible redis - tribution [10]. If proprietary, they often cannot be redistributed, and so such

  2. Potential pitfalls of relying on hydrogen sulfide (H2S) production to identify Salmonella in feed

    Science.gov (United States)

    Salmonella can be difficult to assess and isolate in poultry feed due to stress, uneven distribution and poor growth. Previous studies have shown that several strains of Salmonella can be affected by environmental changes, resulting in H2S-negative colonies. This is a major concern, as H2S productio...

  3. Future Prospects: Scientific Quality Relies on the Critical Reviews from Our Peers

    Institute of Scientific and Technical Information of China (English)

    Chun-Ming Liu

    2009-01-01

    @@ From Executive Editor to Editor-in-Chief is a big jump for me, and means many more responsibilities.I could feel the pressure on my shoulders;from the hopes of my predecessors,my colleagues, and the board members. As a journal with a 57-year history,JIPB has built its credit over the years,publishing many original works in both Chinese and English.However,in the international scientific community,JIPB is still a minor player, especially under the current not-so-healthy impact factor-driven publishing environment.

  4. Evaluating Parental Disagreement in ADHD Diagnosis: Can We Rely on a Single Report From Home?

    Science.gov (United States)

    Caye, Arthur; Machado, Julia D; Rohde, Luís A

    2017-05-01

    Few studies assessed factors associated with the agreement/disagreement between fathers and mothers when rating ADHD symptoms of their offspring. Teachers and both parents assessed a referred sample of 98 children and adolescents aged 6 to 16 years ( M age = 9.79, SD = 2.59) using the Swanson, Nolan, and Pelham (SNAP-IV) rating scale. The agreement was assessed for each of the items of the scale and correlated with variables measuring children's features, socioeconomic adversity, family functioning, and parental psychopathology. Mean agreement between parents was moderate for the inattentive and good for the hyperactive-impulsive construct. Mothers tended to report more symptoms than fathers. The agreement was lower in those families where parents had discrepant educational levels. Our findings suggest a significant cross-informant disagreement between parents on symptoms of ADHD. Discrepant parental education has a relevant role in explaining parental disagreement in reporting ADHD symptoms.

  5. How do groundwater-dependent lakes react if the aquifer they rely on is being pumped?

    Science.gov (United States)

    Vainu, Marko; Terasmaa, Jaanus

    2015-04-01

    Groundwater is a valuable source of drinking water, but at the same time it is the primary contributor to the existence of many surface water bodies. If the latter truth is overlooked in water resources management, and ground- and surface water are not considered as a single resource, then the sustainability of groundwater-dependent ecosystems will become under threat. The necessity for implementing an integrated management of ground- and surface water has also been stressed in the EU Water Framework Directive. This study aims to evaluate the effect of increased groundwater abstraction to groundwater and lake levels; and to evaluate the effect of increased groundwater abstraction to the seepage patterns in one example lake. The Kurtna Lake District in northeastern Estonia contains almost 40 small lakes which are situated in and around the Kurtna Kame Field and constitute an EU Special Area of Conservation. The sands that form the kame field contain a Quaternary groundwater aquifer. Water has been pumped from the aquifer for household use with varying rates since the 1970s, but starting from the summer of 2012 the average pumping rate was increased by 51% compared to the year before. During the current study the water levels of five lakes were monitored regularly from May 2012 to June 2013 - before and after the increase in the pumping rate. The water levels dropped 0.3 to 0.7 m during the year in three closed-basin lakes closest to the abstraction wells, but did not change neither in a flow-through lake nor in a closed-basin lake situated 1.6 km from the wells. Groundwater level in the aquifer (monitored by the Estonian Geological Survey) dropped up to 0.8 m near the abstraction wells in the course of the year, but did not change further from the wells. The estimates of average annual groundwater recharge were derived for the twelve months before both June 2012 and June 2013. Although the recharge rate was lower in the first year, the water-level drop was nevertheless caused by the higher pumping rate, because the effects of lower recharge would have affected the whole aquifer, not just a part of it. At the beginning of June 2012 and June 2013 also lake-bed seepage was directly measured in the bottom of one of the lakes (Lake Martiska). Seepage measurements in the first year showed that outseepage from L. Martiska occurred in the northern part of the lake, which is situated closest to the abstraction wells. For the second year the area of outseepage had widened towards the western shore of the lake. Therefore the increased abstraction rate had initiated the shrinking of these lakes near the abstraction wells that were hydraulically connected to the aquifer. The results of the rare opportunity to analyse lake-groundwater interactions both before and after increased human intervention provide a cautionary example of what may happen to and in groundwater-dependent lakes if the surface water-groundwater interactions are not fully clarified before the intervention.

  6. A Semantic Representation of Adult Learner's Self Awareness relying On Learner's Developing Conceptions of Learning

    DEFF Research Database (Denmark)

    Badie, Farshad

    2016-01-01

    Learning is the reflective activity that enables the learner to draw upon her/his previous experiences and background knowledge to conceptualise, realise, understand and evaluate the present, so as to shape her/his future actions and to construct and develop new knowledge for her(him)self. Learning...... strongly depends on meetings of awarenesses (or self realisations) which we see as achieved through the experiences that mentors and learners undertake jointly. Self realisation is a type of self organisation process and always organises itself. In fact, self realisation is always going to be continued...

  7. Why science? to know, to understand, and to rely on results

    CERN Document Server

    Newton, Roger G

    2012-01-01

    This book aims to describe, for readers uneducated in science, the development of humanity's desire to know and understand the world around us through the various stages of its development to the present, when science is almost universally recognized - at least in the Western world - as the most reliable way of knowing. The book describes the history of the large-scale exploration of the surface of the earth by sea, beginning with the Vikings and the Chinese, and of the unknown interiors of the American and African continents by foot and horseback. After the invention of the telescope, visual exploration of the surfaces of the Moon and Mars were made possible, and finally a visit to the Moon. The book then turns to our legacy from the ancient Greeks of wanting to understand rather than just know, and why the scientific way of understanding is valued. For concreteness, it relates the lives and accomplishments of six great scientists, four from the nineteenth century and two from the twentieth. Finally, the boo...

  8. Urinary concentration does not exclusively rely on plasma vasopressin. A study between genders

    DEFF Research Database (Denmark)

    Graugaard-Jensen, Charlotte; Hvistendahl, Gitte M; Frøkiaer, Jørgen

    2014-01-01

    inpatient study under standardized conditions for measurements of plasma vasopressin, oxytocin, sodium and osmolality. Urine was fractionally collected for measurements of electrolytes, aquaporin-2 and prostaglandin E2. ResultsPlasma vasopressin expressed a diurnal rhythm with a night-time increase in both......AimWe investigated the influence of gender on the diurnal regulation of urine production with special focus on vasopressin, oxytocin and prostaglandin E2. MethodsFifteen young women in mid-follicular phase and 22 young men (20-33years) were included. All participants underwent a 24-h circadian...

  9. 77 FR 39561 - Advanced Braking Technologies That Rely on Forward-Looking Sensors; Request for Comments

    Science.gov (United States)

    2012-07-03

    ... test scenarios: driving over a 1-inch thick steel plate lying flat on the pavement (a plate often used..., NHTSA intends to use a rigid mechanical link between the surrogate vehicle and the towing vehicle to...

  10. Gustatory Habituation in "Drosophila" Relies on "Rutabaga" (Adenylate Cyclase)-Dependent Plasticity of GABAergic Inhibitory Neurons

    Science.gov (United States)

    Paranjpe, Pushkar; Rodrigues, Veronica; VijayRaghavan, K.; Ramaswami, Mani

    2012-01-01

    In some situations, animals seem to ignore stimuli which in other contexts elicit a robust response. This attenuation in behavior, which enables animals to ignore a familiar, unreinforced stimulus, is called habituation. Despite the ubiquity of this phenomenon, it is generally poorly understood in terms of the underlying neural circuitry. Hungry…

  11. The "contemporary synthesis": when politically inclusive genomic science relies on biological notions of race.

    Science.gov (United States)

    Fullwiley, Duana

    2014-12-01

    This essay outlines the emergence of a contemporary synthesis regarding racial thinking in genetic science and in society more broadly. A departure from what Julian Huxley in 1942 termed the "modern synthesis," the contemporary version does not purport to leave race thinking behind in favor of evolution, population genetics, and population-based accounts of natural selection and human diversity. Specifically, the contemporary synthesis blends old concepts (such as that of pure human "types," located within continental land masses) with new attitudes (democratic inclusion, multicultural diversity, and anti-racism). Through various examples, the essay shows how this new synthesis combines ideas about human biological difference that draw on measures of physical characteristics and human genetic material that are both race and population based, yet conflated. This specific amalgam allows old notions of racial types to thrive through conceptual framings that comprise ideas that were once imagined to have the potential to liberate society from racial thinking--and that today remain attached to ideas of progress. As an emergent dynamic, the contemporary synthesis holds the possibility of reinvigorating racism, while simultaneously possessing the potential to promote antiracist science education, disease awareness, and social justice efforts.

  12. Persistent identifiers for web service requests relying on a provenance ontology design pattern

    Science.gov (United States)

    Car, Nicholas; Wang, Jingbo; Wyborn, Lesley; Si, Wei

    2016-04-01

    Delivering provenance information for datasets produced from static inputs is relatively straightforward: we represent the processing actions and data flow using provenance ontologies and link to stored copies of the inputs stored in repositories. If appropriate detail is given, the provenance information can then describe what actions have occurred (transparency) and enable reproducibility. When web service-generated data is used by a process to create a dataset instead of a static inputs, we need to use sophisticated provenance representations of the web service request as we can no longer just link to data stored in a repository. A graph-based provenance representation, such as the W3C's PROV standard, can be used to model the web service request as a single conceptual dataset and also as a small workflow with a number of components within the same provenance report. This dual representation does more than just allow simplified or detailed views of a dataset's production to be used where appropriate. It also allow persistent identifiers to be assigned to instances of a web service requests, thus enabling one form of dynamic data citation, and for those identifiers to resolve to whatever level of detail implementers think appropriate in order for that web service request to be reproduced. In this presentation we detail our reasoning in representing web service requests as small workflows. In outline, this stems from the idea that web service requests are perdurant things and in order to most easily persist knowledge of them for provenance, we should represent them as a nexus of relationships between endurant things, such as datasets and knowledge of particular system types, as these endurant things are far easier to persist. We also describe the ontology design pattern that we use to represent workflows in general and how we apply it to different types of web service requests. We give examples of specific web service requests instances that were made by systems at Australia's National Computing Infrastructure and show how one can 'click' through provenance interfaces to see the dual representations of the requests using provenance management tooling we have built.

  13. Thought on Establishment of the Base of Agricultural Headquarters Rely on the Brand-agriculture Exhibition——Case Study on Seeds Industry Headquarter Bases in Fengtai District%对依托品牌农业会展打造农业总部基地的思考——以打造北京丰台种业总部基地为例

    Institute of Scientific and Technical Information of China (English)

    马俊哲

    2012-01-01

    In order to use the superior resources of urban and rural areas to develop modern agriculture, especially the modern seeds industry, we should used the idea of headquarters economy and the influence of Beijing seeds congress brand effect, established seeds industry headquarter bases in Fengtai District, formed the model of seeds industry headquarter bases, so that we could accelerate the construction of the capital of seeds industry in Beijing. The results showed that compared with the disadvantages the advantages of rely on the brand effect of Beijing seeds congress to establish seeds industry headquarter bases in Fengtai district were more, and many disadvantages could turned into advantages by efforts, such as small scale of seeds enterprise, high cost of entering, strong regional of seeds production and sales, etc. These Problems could be solved by enterprise annexing, improving management and enlarging the influence of exhibition. In order to accelerate seeds industry headquarter bases in Fengtai District, we should pay attention to definite the regional range and function orientation of seeds industry headquarter bases in Fengtai District, develop leading enterprise, introduced the seeds enterprises headquarter, strengthen the power of policy support.%为了充分有效地和用城乡优势资源发展现代农业特别是现代种业,运用总部经济的发展理念,并借助具有20年历史和影响的北京种子大会的品牌效应,打造丰台种业总部基地,形成企业内部在空间上分离且耦合的总部种业发展模式,以加快北京的“种业之都”建设.研究表明:依托北京种子大会品牌打造丰台种业总部基地发展总部种业的有利条件多于不利条件的,而且许多不利条件都可以通过自身努力转化为有利条件,如种业企业规模小、入驻成本高、种子生产销售区域性强等,都可以通过引导种业企业购并、改善经营管理和进一步扩大展会影响面等予以

  14. Research of SSO between Lotus Domino and WebSphere%Lotus Domino和WebSphere平台上单点登录技术的研究与实现

    Institute of Scientific and Technical Information of China (English)

    杨兆赞

    2004-01-01

    实现跨 WebSphere 和 Domino 服务器的单点登录,使得公司能够结合利用 WebSphere 服务器在事务方面的优势和 Domino 服务器在协作方面的优势,由此产生的 Web 应用同时扩展了WebSphere环境和Domino环境的性能.本文对实现单点登录功能的技术原理进行了研究,同时提出了具体实现方法.

  15. Influence of chromium on the initial corrosion behavior of low alloy steels in the CO2-O2-H2S-SO2 wet-dry corrosion environment of cargo oil tankers

    Institute of Scientific and Technical Information of China (English)

    Qing-he Zhao; Wei Liu; Jie Zhao; Dong Zhang; Peng-cheng Liu; Min-xu Lu

    2015-01-01

    The influence of Cr on the initial corrosion behavior of low-alloy steels exposed to a CO2–O2–H2S–SO2wet–dry corrosion envi-ronment was investigated using weight-loss measurements, scanning electron microscopy, N2 adsorption tests, X-ray diffraction analysis, and electrochemical impedance spectroscopy. The results show that the corrosion rate increases with increasing Cr content in samples subjected to corrosion for 21 d. However, the rust grain size decreases, its specific surface area increases, and it becomes more compact and denser with increasing Cr content, which indicates the enhanced protectivity of the rust. The results of charge transfer resistance (Rct) calculations indicate that higher Cr contents can accelerate the corrosion during the first 7 d and promote the formation of the enhanced protective inner rust after 14 d; the formed protective inner rust is responsible for the greater corrosion resistance during long-term exposure.

  16. Standardized observation of neighbourhood disorder: does it work in Canada?

    Directory of Open Access Journals (Sweden)

    Zaidi Qamar

    2010-02-01

    Full Text Available Abstract Background There is a growing body of evidence that where you live is important to your health. Despite numerous previous studies investigating the relationship between neighbourhood deprivation (and structure and residents' health, the precise nature of this relationship remains unclear. Relatively few investigations have relied on direct observation of neighbourhoods, while those that have were developed primarily in US settings. Evaluation of the transferability of such tools to other contexts is an important first step before applying such instruments to the investigation of health and well-being. This study evaluated the performance of a systematic social observational (SSO tool (adapted from previous studies of American and British neighbourhoods in a Canadian urban context. Methods This was a mixed-methods study. Quantitative SSO ratings and qualitative descriptions of 176 block faces were obtained in six Toronto neighbourhoods (4 low-income, and 2 middle/high-income by trained raters. Exploratory factor analysis was conducted with the quantitative SSO ratings. Content analysis consisted of independent coding of qualitative data by three members of the research team to yield common themes and categories. Results Factor analysis identified three factors (physical decay/disorder, social accessibility, recreational opportunities, but only 'physical decay/disorder' reflected previous findings in the literature. Qualitative results (based on raters' fieldwork experiences revealed the tool's shortcomings in capturing important features of the neighbourhoods under study, and informed interpretation of the quantitative findings. Conclusions This study tested the performance of an SSO tool in a Canadian context, which is an important initial step before applying it to the study of health and disease. The tool demonstrated important shortcomings when applied to six diverse Toronto neighbourhoods. The study's analyses challenge previously

  17. Sorbitan sesquioleate; a rare cause of contact allergy in consecutively patch tested dermatitis patients

    DEFF Research Database (Denmark)

    Bennike, Niels Højsager; Johansen, Jeanne Duus

    2016-01-01

    BACKGROUND: Sorbitan sesquioleate (SSO) has been added to fragrance mix I (FM I) as an emulsifier since the 1990s. Being a contact allergen itself, SSO has the potential to cause false-positive reactions to FM I. Recent results obtained with selected FM I-positive patients have shown that 5% have...... concomitant positive reactions to SSO. OBJECTIVES: To investigate the 5-year prevalence of contact allergy to SSO and evaluate the importance of SSO allergy when patch test results for FM I are interpreted. METHODS: This was a retrospective database study of consecutively patch tested eczema patients (n = 4 6...... 3 7) from 2010 to 2014. All patients were tested with our baseline series including FM I and SSO 20% in pet. RESULTS: Sensitization to SSO was identified in 9 (0.2%) patients. The proportion of FM I-positive patients with concomitant positive reactions to SSO was 1.4%. CONCLUSIONS: SSO is a rare...

  18. Training college students' creative team rely-ing on the opening laboratory%依托实验室开放加强大学生创新团队的培养

    Institute of Scientific and Technical Information of China (English)

    耿欣

    2015-01-01

    实验室开放对大学生团队建设提供了肥沃的土壤。本文结合我校实验室开放的现状,针对大学生创新团队的活动,从团队的建设与管理、团队活动项目的设置等方面进行了总结与分析,为大学生创新团队的活动提供了新的思路。%Open laboratory provides fertile soil on the team construction of College students. In light of the current situation of our laboratory, according to the college students' innovation team activities, from construction and management, team project settings are summarized and analyzed, and provides a new idea for college students' innovative team activities.

  19. Mycobacterium leprae intracellular survival relies on cholesterol accumulation in infected macrophages: a potential target for new drugs for leprosy treatment

    Science.gov (United States)

    Mattos, Katherine A; Oliveira, Viviane C G; Berrêdo-Pinho, Marcia; Amaral, Julio J; Antunes, Luis Caetano M; Melo, Rossana C N; Acosta, Chyntia C D; Moura, Danielle F; Olmo, Roberta; Han, Jun; Rosa, Patricia S; Almeida, Patrícia E; Finlay, B Brett; Borchers, Christoph H; Sarno, Euzenir N; Bozza, Patricia T; Atella, Georgia C; Pessolani, Maria Cristina V

    2014-01-01

    We recently showed that Mycobacterium leprae (ML) is able to induce lipid droplet formation in infected macrophages. We herein confirm that cholesterol (Cho) is one of the host lipid molecules that accumulate in ML-infected macrophages and investigate the effects of ML on cellular Cho metabolism responsible for its accumulation. The expression levels of LDL receptors (LDL-R, CD36, SRA-1, SR-B1, and LRP-1) and enzymes involved in Cho biosynthesis were investigated by qRT-PCR and/or Western blot and shown to be higher in lepromatous leprosy (LL) tissues when compared to borderline tuberculoid (BT) lesions. Moreover, higher levels of the active form of the sterol regulatory element-binding protein (SREBP) transcriptional factors, key regulators of the biosynthesis and uptake of cellular Cho, were found in LL skin biopsies. Functional in vitro assays confirmed the higher capacity of ML-infected macrophages to synthesize Cho and sequester exogenous LDL-Cho. Notably, Cho colocalized to ML-containing phagosomes, and Cho metabolism impairment, through either de novo synthesis inhibition by statins or depletion of exogenous Cho, decreased intracellular bacterial survival. These findings highlight the importance of metabolic integration between the host and bacteria to leprosy pathophysiology, opening new avenues for novel therapeutic strategies to leprosy. PMID:24552180

  20. The neuroimmune changes induced by cohabitation with an Ehrlich tumor-bearing cage mate rely on olfactory information.

    Science.gov (United States)

    Alves, Glaucie J; Ribeiro, Alison; Palermo-Neto, João

    2012-01-01

    Cohabitation for 14 days with Ehrlich tumor-bearing mice was shown to increase locomotor activity, to decrease hypothalamic noradrenaline (NA) levels, to increase NA turnover and to decrease innate immune responses and decrease the animals' resistance to tumor growth. Cage mates of a B16F10 melanoma-bearer mice were also reported to show neuroimmune changes. Chemosignals released by Ehrlich tumor-bearing mice have been reported to be relevant for the neutrophil activity changes induced by cohabitation. The present experiment was designed to further analyze the effects of odor cues on neuroimmune changes induced by cohabitation with a sick cage mate. Specifically, the relevance of chemosignals released by an Ehrlich tumor-bearing mouse was assessed on the following: behavior (open-field and plus maze); hypothalamic NA levels and turnover; adrenaline (A) and NA plasmatic levels; and host resistance induced by tumor growth. To comply with such objectives, devices specifically constructed to analyze the influence of chemosignals released from tumor-bearing mice were employed. The results show that deprivation of odor cues released by Ehrlich tumor-bearing mice reversed the behavioral, neurochemical and immune changes induced by cohabitation. Mice use scents for intraspecies communication in many social contexts. Tumors produce volatile organic compounds released into the atmosphere through breath, sweat, and urine. Our results strongly suggest that volatile compounds released by Ehrlich tumor-injected mice are perceived by their conspecifics, inducing the neuroimmune changes reported for cohabitation with a sick companion. Copyright © 2011 Elsevier Inc. All rights reserved.

  1. Analysis of complete genomes suggests that many prokaryotes do not rely on hairpin formation in transcription termination

    National Research Council Canada - National Science Library

    Washio, T; Sasayama, J; Tomita, M

    1998-01-01

    Free energy values of mRNA tertiary structures around stop codons were systematically calculated to surmise the hairpin-forming potential for all genes in each of the 16 complete prokaryote genomes...

  2. Adaptation of the symbiotic Mesorhizobium-chickpea relationship to phosphate deficiency relies on reprogramming of whole-plant metabolism

    National Research Council Canada - National Science Library

    Nasr Esfahani, Maryam; Kusano, Miyako; Nguyen, Kien Huu; Watanabe, Yasuko; Ha, Chien Van; Saito, Kazuki; Sulieman, Saad; Herrera-Estrella, Luis; Tran, L S

    2016-01-01

    .... To elucidate the mechanisms involved in nodule acclimation to low Pi availability, two Mesorhizobium-chickpea associations exhibiting differential symbiotic performances, Mesorhizobium ciceri CP-31 (McCP-31...

  3. Gephyrin-mediated γ-aminobutyric acid type A and glycine receptor clustering relies on a common binding site

    DEFF Research Database (Denmark)

    Maric, Hans-Michael; Mukherjee, Jayanta; Tretter, Verena

    2011-01-01

    Gephyrin is the major protein determinant for the clustering of inhibitory neurotransmitter receptors. Earlier analyses revealed that gephyrin tightly binds to residues 398-410 of the glycine receptor β subunit (GlyR β) and, as demonstrated only recently, also interacts with GABA(A) receptors (GABA......(A)Rs) containing the α1, α2, and α3 subunits. Here, we dissect the molecular basis underlying the interactions between gephyrin and GABA(A)Rs containing these α-subunits and compare them to the crystal structure of the gephyrin-GlyR β complex. Biophysical and biochemical assays revealed that, in contrast to its...... that are central for gephyrin binding. Consistent with the biochemical data, mutations of the corresponding residues within the cytoplasmic domain of α2 subunit-containing GABA(A)Rs attenuated clustering of these receptors at postsynaptic sites in hippocampal neurons. Taken together, our experiments provide key...

  4. Catalytic ozonation of oxalate with a cerium supported palladium oxide: An efficient degradation not relying on hydroxyl radical oxidation

    KAUST Repository

    Zhang, Tao

    2011-11-01

    The cerium supported palladium oxide (PdO/CeO 2) at a low palladium loading was found very effective in catalytic ozonation of oxalate, a probe compound that is difficult to be efficiently degraded in water with hydroxyl radical oxidation and one of the major byproducts in ozonation of organic matter. The oxalate was degraded into CO 2 during the catalytic ozonation. The molar ratio of oxalate degraded to ozone consumption increased with increasing catalyst dose and decreasing ozone dosage and pH under the conditions of this study. The maximum molar ratio reached around 1, meaning that the catalyst was highly active and selective for oxalate degradation in water. The catalytic ozonation, which showed relatively stable activity, does not promote hydroxyl radical generation from ozone. Analysis with ATR-FTIR and in situ Raman spectroscopy revealed that 1) oxalate was adsorbed on CeO 2 of the catalyst forming surface complexes, and 2) O 3 was adsorbed on PdO of the catalyst and further decomposed to surface atomic oxygen (*O), surface peroxide (*O 2), and O 2 gas in sequence. The results indicate that the high activity of the catalyst is related to the synergetic function of PdO and CeO 2 in that the surface atomic oxygen readily reacts with the surface cerium-oxalate complex. This kind of catalytic ozonation would be potentially effective for the degradation of polar refractory organic pollutants and hydrophilic natural organic matter. © 2011 American Chemical Society.

  5. Enterocyte loss of polarity and gut wound healing rely upon the F-actin-severing function of villin

    Science.gov (United States)

    Efficient wound healing is required to maintain the integrity of the intestinal epithelial barrier because of its constant exposure to a large variety of environmental stresses. This process implies a partial cell depolarization and the acquisition of a motile phenotype that involves rearrangements ...

  6. Do Adults with Cochlear Implants Rely on Different Acoustic Cues for Phoneme Perception than Adults with Normal Hearing?

    Science.gov (United States)

    Moberly, Aaron C.; Lowenstein, Joanna H.; Tarr, Eric; Caldwell-Tarr, Amanda; Welling, D. Bradley; Shahin, Antoine J.; Nittrouer, Susan

    2014-01-01

    Purpose: Several acoustic cues specify any single phonemic contrast. Nonetheless, adult, native speakers of a language share weighting strategies, showing preferential attention to some properties over others. Cochlear implant (CI) signal processing disrupts the salience of some cues: In general, amplitude structure remains readily available, but…

  7. Early CT perfusion mismatch in acute stroke is not time-dependent but relies on collateralization grade

    Energy Technology Data Exchange (ETDEWEB)

    Baumgarten, Louisa von; Straube, Andreas [University of Munich Hospitals, Department of Neurology, Munich (Germany); Thierfelder, Kolja M.; Beyer, Sebastian E.; Baumann, Alena B.; Bollwein, Christine; Reiser, Maximilian F.; Sommer, Wieland H. [Ludwig-Maximilians-University Hospital Munich, Institute for Clinical Radiology, Munich (Germany); Janssen, Hendrik [Ludwig-Maximilians-University Hospital Munich, Department of Neuroradiology, Munich (Germany)

    2016-04-15

    Factors that determine the extent of the penumbra in the initial diagnostic workup using whole brain CT Perfusion (WB-CTP) remain unclear. The purpose of the current study was to determine a possible dependency of the initial mismatch size between cerebral blood flow (CBF) and cerebral blood volume (CBV) from time after symptom onset, leptomeningeal collateralization, and occlusion localization in acute middle cerebral artery (MCA) infarctions. Out of an existing cohort of 992 consecutive patients receiving multiparametric CT scans including WB-CTP due to suspected stroke, we included patients who had (1) a witnessed time of symptom onset, (2) an infarction of the MCA territory as documented by follow-up imaging, and (3) an initial CBF volume of >10 ml. CBF and CBV lesion sizes, collateralization grade, and the site of occlusion were determined. We included 103 patients. Univariate analysis showed that time from symptom onset (168 +/- 91.2 min) did not correlate with relative or absolute mismatch volumes (p = 0.458 and p = 0.921). Higher collateralization gradings were associated with small absolute mismatch volumes (p = 0.004 and p < 0.001). Internal carotid artery (ICA) occlusions were associated with large absolute mismatch volumes (p = 0.004). Multivariate analysis confirmed that ICA occlusion was associated with large absolute mismatch volumes (p = 0.005), and high collateral grade was associated with small absolute mismatch volumes (p = 0.017). There is no significant correlation between initial CTP mismatch and time after symptom onset. Predictors of mismatch size include the extent of the collaterals and a proximal location of the occlusion. (orig.)

  8. Marketing communications in industrial B2b markets enhancing the value of the corporate brand relying on common added values

    Directory of Open Access Journals (Sweden)

    Jose Ignacio Monrabal

    2013-09-01

    Full Text Available Today, industrial Business-to-Business (B2B markets are mainly characterized by a highly trained customer for making rational decisions in a highly competitive and global market, requiring more than ever organizations to approach their markets with a single and consistent message. Such demanding scenario requires to define a corporate brand transmitting in one message all the advantages that a Customer may appreciate in the long-term, based not only on what the company stands for in the market, but also the benefits of all its products throughout its portfolio. Such elements are referred as Common Added Values (CAVs, being some general examples the technology, quality, innovation and reliability; the capacity, infrastructure and distribution network; after-sales service policies, support and training; or the price and financial policies. This paper tries to explain the source of this need, describing the main differences between a brand communications model focused on the product or including the main B2B corporate values; and highlighting the main CAVs, to get an industry player either small or large, can succeed in generating brand equity through an integrated marketing communications strategy.

  9. Leishmania (Viannia braziliensis growth in vitro culture relies more on folic acid availability than Leihsmania (Leishmania amazonensis

    Directory of Open Access Journals (Sweden)

    Andrea Niño

    2005-05-01

    Full Text Available We compared the in vitro growth of promastigotes from two Leishmania species in TC-100 and Schneider media. Leishmania (Leishmania amazonensis replication rates were similar in both tissue culture media and reached maximum rates by 48 h. In contrast Leishmania (Viannia braziliensis growth was significantly greater in TC-100 but maximum rates were achieved by 96 h. Folic acid appears to be the limiting factor and supplementation of Schneider media with this nutrient improved L. (V. braziliensis replication rates and decreased the time of maximum replication to 48 h.

  10. Organizational Strategic Planning and Execution: Should Governmental Organizations Rely on Strategic Planning for the Success of the Organization?

    Science.gov (United States)

    2007-06-01

    Competition that it faces. 16 The analysis of internal factors will include (IV) the company’s marketing mix ; (V) the internal resources; and (VI) a variety...Government; Economy; Socio-Demography; Technology) III Industry & Competition IV Marketing Mix (Outputs) A B C V Internal Resource

  11. Multiple source genes of HAmo SINE actively expanded and ongoing retroposition in cyprinid genomes relying on its partner LINE

    Directory of Open Access Journals (Sweden)

    Gan Xiaoni

    2010-04-01

    Full Text Available Abstract Background We recently characterized HAmo SINE and its partner LINE in silver carp and bighead carp based on hybridization capture of repetitive elements from digested genomic DNA in solution using a bead-probe 1. To reveal the distribution and evolutionary history of SINEs and LINEs in cyprinid genomes, we performed a multi-species search for HAmo SINE and its partner LINE using the bead-probe capture and internal-primer-SINE polymerase chain reaction (PCR techniques. Results Sixty-seven full-size and 125 internal-SINE sequences (as well as 34 full-size and 9 internal sequences previously reported in bighead carp and silver carp from 17 species of the family Cyprinidae were aligned as well as 14 new isolated HAmoL2 sequences. Four subfamilies (type I, II, III and IV, which were divided based on diagnostic nucleotides in the tRNA-unrelated region, expanded preferentially within a certain lineage or within the whole family of Cyprinidae as multiple active source genes. The copy numbers of HAmo SINEs were estimated to vary from 104 to 106 in cyprinid genomes by quantitative RT-PCR. Over one hundred type IV members were identified and characterized in the primitive cyprinid Danio rerio genome but only tens of sequences were found to be similar with type I, II and III since the type IV was the oldest subfamily and its members dispersed in almost all investigated cyprinid fishes. For determining the taxonomic distribution of HAmo SINE, inter-primer SINE PCR was conducted in other non-cyprinid fishes, the results shows that HAmo SINE- related sequences may disperse in other families of order Cypriniforms but absent in other orders of bony fishes: Siluriformes, Polypteriformes, Lepidosteiformes, Acipenseriformes and Osteoglossiforms. Conclusions Depending on HAmo LINE2, multiple source genes (subfamilies of HAmo SINE actively expanded and underwent retroposition in a certain lineage or within the whole family of Cyprinidae. From this perspective, HAmo SINE should provide useful phylogenetic makers for future analyses of the evolutionary relationships among species in the family Cyprinidae.

  12. Zebrafish second heart field development relies on progenitor specification in anterior lateral plate mesoderm and nkx2.5 function.

    Science.gov (United States)

    Guner-Ataman, Burcu; Paffett-Lugassy, Noelle; Adams, Meghan S; Nevis, Kathleen R; Jahangiri, Leila; Obregon, Pablo; Kikuchi, Kazu; Poss, Kenneth D; Burns, Caroline E; Burns, C Geoffrey

    2013-03-01

    Second heart field (SHF) progenitors perform essential functions during mammalian cardiogenesis. We recently identified a population of cardiac progenitor cells (CPCs) in zebrafish expressing latent TGFβ-binding protein 3 (ltbp3) that exhibits several defining characteristics of the anterior SHF in mammals. However, ltbp3 transcripts are conspicuously absent in anterior lateral plate mesoderm (ALPM), where SHF progenitors are specified in higher vertebrates. Instead, ltbp3 expression initiates at the arterial pole of the developing heart tube. Because the mechanisms of cardiac development are conserved evolutionarily, we hypothesized that zebrafish SHF specification also occurs in the ALPM. To test this hypothesis, we Cre/loxP lineage traced gata4(+) and nkx2.5(+) ALPM populations predicted to contain SHF progenitors, based on evolutionary conservation of ALPM patterning. Traced cells were identified in SHF-derived distal ventricular myocardium and in three lineages in the outflow tract (OFT). We confirmed the extent of contributions made by ALPM nkx2.5(+) cells using Kaede photoconversion. Taken together, these data demonstrate that, as in higher vertebrates, zebrafish SHF progenitors are specified within the ALPM and express nkx2.5. Furthermore, we tested the hypothesis that Nkx2.5 plays a conserved and essential role during zebrafish SHF development. Embryos injected with an nkx2.5 morpholino exhibited SHF phenotypes caused by compromised progenitor cell proliferation. Co-injecting low doses of nkx2.5 and ltbp3 morpholinos revealed a genetic interaction between these factors. Taken together, our data highlight two conserved features of zebrafish SHF development, reveal a novel genetic relationship between nkx2.5 and ltbp3, and underscore the utility of this model organism for deciphering SHF biology.

  13. Diagnosis of sclerosing hemangioma of lung: Don′t rely on fine-needle aspiration cytology diagnosis alone

    Directory of Open Access Journals (Sweden)

    Kaushik Saha

    2013-01-01

    Full Text Available Sclerosing hemangioma is a rare variety of benign pulmonary neoplasm. It usually presents as asymptomatic, solitary, peripheral, circumscribed lesions in middle-aged women. Here, we describe a 46-year-old woman presenting to us for evaluation of right parahilar lung mass. Previous chest radiography done 10 years back showed a lung mass of almost similar size. Computed Tomography (CT-guided fine-needle aspiration cytology (FNAC was suggestive of adenocarcinoma of lung. A well-circumscribed, capsulated, ovoid mass measuring 5.6 cm × 4 cm × 3 cm, adjacent to the transverse fissure of the right lung was excised by lateral thoracotomy. Histopathological examination along with immunohistochemistry was suggestive of sclerosing hemangioma of lung. A pathologist must consider the clinicoradiological features before coming to a final diagnosis of lung malignancy from FNAC. Whenever there is any confusion regarding lung mass, thoracotomy must be done for arriving at an exact diagnosis from histopathology.

  14. Decoding temporal structure in music and speech relies on shared brain resources but elicits different fine-scale spatial patterns.

    Science.gov (United States)

    Abrams, Daniel A; Bhatara, Anjali; Ryali, Srikanth; Balaban, Evan; Levitin, Daniel J; Menon, Vinod

    2011-07-01

    Music and speech are complex sound streams with hierarchical rules of temporal organization that become elaborated over time. Here, we use functional magnetic resonance imaging to measure brain activity patterns in 20 right-handed nonmusicians as they listened to natural and temporally reordered musical and speech stimuli matched for familiarity, emotion, and valence. Heart rate variability and mean respiration rates were simultaneously measured and were found not to differ between musical and speech stimuli. Although the same manipulation of temporal structure elicited brain activation level differences of similar magnitude for both music and speech stimuli, multivariate classification analysis revealed distinct spatial patterns of brain responses in the 2 domains. Distributed neuronal populations that included the inferior frontal cortex, the posterior and anterior superior and middle temporal gyri, and the auditory brainstem classified temporal structure manipulations in music and speech with significant levels of accuracy. While agreeing with previous findings that music and speech processing share neural substrates, this work shows that temporal structure in the 2 domains is encoded differently, highlighting a fundamental dissimilarity in how the same neural resources are deployed.

  15. Can We Rely on Predicted Basal Metabolic Rate in Patients With Intestinal Failure on Home Parenteral Nutrition?

    DEFF Research Database (Denmark)

    Andersen, Anders Skallerup; Pedersen, Louis Nygaard; Olesen, Søren Schou

    2017-01-01

    evaluated the accuracy of predicted BMR in clinically stable patients with IF dependent on home parenteral nutrition (HPN). METHODS: In total, 103 patients with IF were included. We used indirect calorimetry for assessment of BMR and calculated predicted BMR using different equations based on anthropometric......BACKGROUND AND AIMS: Intestinal failure (IF) is a serious and common complication of short bowel syndrome with patients depending on parental nutrition (PN) support. Effective nutrition management requires an accurate estimation of the patient's basal metabolic rate (BMR) to avoid underfeeding...

  16. Mast cell tryptase stimulates myoblast proliferation; a mechanism relying on protease-activated receptor-2 and cyclooxygenase-2

    Directory of Open Access Journals (Sweden)

    Côté Claude H

    2011-10-01

    Full Text Available Abstract Background Mast cells contribute to tissue repair in fibrous tissues by stimulating proliferation of fibroblasts through the release of tryptase which activates protease-activated receptor-2 (PAR-2. The possibility that a tryptase/PAR-2 signaling pathway exists in skeletal muscle cell has never been investigated. The aim of this study was to evaluate whether tryptase can stimulate myoblast proliferation and determine the downstream cascade. Methods Proliferation of L6 rat skeletal myoblasts stimulated with PAR-2 agonists (tryptase, trypsin and SLIGKV was assessed. The specificity of the tryptase effect was evaluated with a specific inhibitor, APC-366. Western blot analyses were used to evaluate the expression and functionality of PAR-2 receptor and to assess the expression of COX-2. COX-2 activity was evaluated with a commercial activity assay kit and by measurement of PGF2α production. Proliferation assays were also performed in presence of different prostaglandins (PGs. Results Tryptase increased L6 myoblast proliferation by 35% above control group and this effect was completely inhibited by APC-366. We confirmed the expression of PAR-2 receptor in vivo in skeletal muscle cells and in satellite cells and in vitro in L6 cells, where PAR-2 was found to be functional. Trypsin and SLIGKV increased L6 cells proliferation by 76% and 26% above control, respectively. COX-2 activity was increased following stimulation with PAR-2 agonist but its expression remained unchanged. Inhibition of COX-2 activity by NS-398 abolished the stimulation of cell proliferation induced by tryptase and trypsin. Finally, 15-deoxy-Δ-12,14-prostaglandin J2 (15Δ-PGJ2, a product of COX-2-derived prostaglandin D2, stimulated myoblast proliferation, but not PGE2 and PGF2α. Conclusions Taken together, our data show that tryptase can stimulate myoblast proliferation and this effect is part of a signaling cascade dependent on PAR-2 activation and on the downstream activation of COX-2.

  17. Mast cell tryptase stimulates myoblast proliferation; a mechanism relying on protease-activated receptor-2 and cyclooxygenase-2

    OpenAIRE

    Côté Claude H; Tremblay Marie-Hélène; Duchesne Elise

    2011-01-01

    Abstract Background Mast cells contribute to tissue repair in fibrous tissues by stimulating proliferation of fibroblasts through the release of tryptase which activates protease-activated receptor-2 (PAR-2). The possibility that a tryptase/PAR-2 signaling pathway exists in skeletal muscle cell has never been investigated. The aim of this study was to evaluate whether tryptase can stimulate myoblast proliferation and determine the downstream cascade. Methods Proliferation of L6 rat skeletal m...

  18. PARKINSON'S DISEASE PATIENTS WITH DOMINANT HEMIBODY AFFECTED BY THE DISEASE RELY MORE ON VISION TO MAINTAIN UPRIGHT POSTURAL CONTROL.

    Science.gov (United States)

    Lahr, Juliana; Pereira, Marcelo Pinto; Pelicioni, Paulo Henrique Silva; De Morais, Luana Carolina; Gobbi, Lilian Teresa Bucken

    2015-12-01

    This study assesses the association between disease onset side (dominant or non-dominant) and vision on postural control of Parkinson's disease patients. Patient volunteers composed two groups, according to the onset side affected: Dominant group (n=9; M age=66.1 yr., SD=7.2; 6 women, 3 men) and Non-dominant group (n=9; M age=67.4 yr., SD=6.4; 6 women, 3 men). The groups' postural control was assessed by posturography during quiet upright stance in two conditions, Eyes open and Eyes closed. Two-way analyses of variance (ANOVAs; group×condition) with repeated measures for the second factor assessed the differences associated with affected hemibody and vision on postural control. Analyses indicated that patients with the dominant side affected also presented significantly greater variation in center of pressure than those with the non-dominant side affected, mainly in the Eyes closed condition. The results demonstrate a higher reliance on vision in the dominant side, possibly to compensate somatosensory system impairments. These results also highlight the importance of analyzing the hemibody affected by the disease when postural control is assessed in this population.

  19. Terminal axonal arborization and synaptic bouton formation critically rely on abp1 and the arp2/3 complex.

    Directory of Open Access Journals (Sweden)

    Nicole Koch

    Full Text Available Neuronal network formation depends on properly timed and localized generation of presynaptic as well as postsynaptic structures. Although of utmost importance for understanding development and plasticity of the nervous system and neurodegenerative diseases, the molecular mechanisms that ensure the fine-control needed for coordinated establishment of pre- and postsynapses are still largely unknown. We show that the F-actin-binding protein Abp1 is prominently expressed in the Drosophila nervous system and reveal that Abp1 is an important regulator in shaping glutamatergic neuromuscular junctions (NMJs of flies. STED microscopy shows that Abp1 accumulations can be found in close proximity of synaptic vesicles and at the cell cortex in nerve terminals. Abp1 knock-out larvae have locomotion defects and underdeveloped NMJs that are characterized by a reduced number of both type Ib synaptic boutons and branches of motornerve terminals. Abp1 is able to indirectly trigger Arp2/3 complex-mediated actin nucleation and interacts with both WASP and Scar. Consistently, Arp2 and Arp3 loss-of-function also resulted in impairments of bouton formation and arborization at NMJs, i.e. fully phenocopied abp1 knock-out. Interestingly, neuron- and muscle-specific rescue experiments revealed that synaptic bouton formation critically depends on presynaptic Abp1, whereas the NMJ branching defects can be compensated for by restoring Abp1 functions at either side. In line with this presynaptic importance of Abp1, also presynaptic Arp2 and Arp3 are crucial for the formation of type Ib synaptic boutons. Interestingly, presynaptic Abp1 functions in NMJ formation were fully dependent on the Arp2/3 complex, as revealed by suppression of Abp1-induced synaptic bouton formation and branching of axon terminals upon presynaptic Arp2 RNAi. These data reveal that Abp1 and Arp2/3 complex-mediated actin cytoskeletal dynamics drive both synaptic bouton formation and NMJ branching. Our data furthermore shed light on an intense bidirectional functional crosstalk between pre- and postsynapses during the development of synaptic contacts.

  20. Instructive Bilingualism: Can Bilingual Children with Specific Language Impairment Rely on One Language in Learning a Second One?

    Science.gov (United States)

    Armon-Lotem, Sharon

    2010-01-01

    Only a decade ago, a very few researchers considered the study of language disorders in bilingual population worth pursuing. It was mostly argued that there were enough challenges in studying bilingualism, and even more challenges in the study of specific language impairment (SLI). So why complicate things and combine the two domains?

  1. Paper-based biosensor relying on flower-like reduced graphene guided enzymatically deposition of polyaniline for Pb(2+) detection.

    Science.gov (United States)

    Ge, Shenguang; Wu, Kaiqing; Zhang, Yan; Yan, Mei; Yu, Jinghua

    2016-06-15

    A multi-amplified paper-based electrochemical strategy using Pb(2+) dependent DNAzyme as the recognition unit for Pb(2+) detection was developed. In this work, flower-like reduced graphene (FrGO) was prepared utilizing flower-like ZnO as template, which was first one step grown on the gold nanoparticles modified paper working electrode (Au-PWE). After being treated with acid and then modified with Au, a novel sensor platform named Au/FrGO/Au-PWE with large specific surface area and good electrical conductivity was fabricated. The Mn2O3 nanoparticle-assembled hierarchical hollow spheres (H-Mn2O3) was served as nanocarrier to immobilize GOx, HRP and signal strand (S3), resulting to the formation of S3/H-Mn2O3/HRP/GOx bioconjugations. In the presence of Pb(2+), the DNAzyme (S1) was activated and the substrate strand (S2) was cleaved. After the incubation with S3/H-Mn2O3/HRP/GOx in 0.1M HAc-NaAc solution (pH 4.3) containing 30 mM aniline and 15 mM glucose, a readily measurable "turn-on" electrochemical signal could be measured. On the basis of the signal amplification strategy of Au/FrGO/Au-PWE sensing platform and S3/H-Mn2O3/HRP/GOx bioconjugations, the developed biosensor exhibited a good linear response toward over a wide range of concentration from 0.005 to 2000 nM.

  2. Development of a millimetrically scaled biodiesel transesterification device that relies on droplet-based co-axial fluidics

    Science.gov (United States)

    Yeh, S. I.; Huang, Y. C.; Cheng, C. H.; Cheng, C. M.; Yang, J. T.

    2016-07-01

    In this study, we investigated a fluidic system that adheres to new concepts of energy production. To improve efficiency, cost, and ease of manufacture, a millimetrically scaled device that employs a droplet-based co-axial fluidic system was devised to complete alkali-catalyzed transesterification for biodiesel production. The large surface-to-volume ratio of the droplet-based system, and the internal circulation induced inside the moving droplets, significantly enhanced the reaction rate of immiscible liquids used here – soybean oil and methanol. This device also decreased the molar ratio between methanol and oil to near the stoichiometric coefficients of a balanced chemical equation, which enhanced the total biodiesel volume produced, and decreased the costs of purification and recovery of excess methanol. In this work, the droplet-based co-axial fluidic system performed better than other methods of continuous-flow production. We achieved an efficiency that is much greater than that of reported systems. This study demonstrated the high potential of droplet-based fluidic chips for energy production. The small energy consumption and low cost of the highly purified biodiesel transesterification system described conforms to the requirements of distributed energy (inexpensive production on a moderate scale) in the world.

  3. Design of a Simple and Modular 2-DOF Ankle Physiotherapy Device Relying on a Hybrid Serial-Parallel Robotic Architecture

    Directory of Open Access Journals (Sweden)

    Christos E. Syrseloudis

    2011-01-01

    Full Text Available The aim of this work is to propose a new 2-DOF robotic platform with hybrid parallel-serial structure and to undertake its parametric design so that it can follow the whole range of ankle related foot movements. This robot can serve as a human ankle rehabilitation device. The existing ankle rehabilitation devices present typically one or more of the following shortcomings: redundancy, large size, or high cost, hence the need for a device that could offer simplicity, modularity, and low cost of construction and maintenance. In addition, our targeted device must be safe during operation, disallow undesirable movements of the foot, while adaptable to any human foot. Our detailed study of foot kinematics has led us to a new hybrid architecture, which strikes a balance among all aforementioned goals. It consists of a passive serial kinematics chain with two adjustable screws so that the axes of the chain match the two main ankle-axes of typical feet. An active parallel chain, which consists of two prismatic actuators, provides the movement of the platform. Thus, the platform can follow the foot movements, thanks to the passive chain, and also possesses the advantages of parallel robots, including rigidity, high stiffness and force capabilities. The lack of redundancy yields a simpler device with lower size and cost. The paper describes the kinematics modelling of the platform and analyses the force and velocity transmission. The parametric design of the platform is carried out; our simulations confirm the platform's suitability for ankle rehabilitation.

  4. Reducing costs of managing and accessing navigation and ancillary data by relying on the extensive capabilities of NASA's spice system

    Science.gov (United States)

    Semenov, Boris V.; Acton, Charles H., Jr.; Bachman, Nathaniel J.; Elson, Lee S.; Wright, Edward D.

    2005-01-01

    The SPICE system of navigation and ancillary data possesses a number of traits that make its use in modern space missions of all types highly cost efficient. The core of the system is a software library providing API interfaces for storing and retrieving such data as trajectories, orientations, time conversions, and instrument geometry parameters. Applications used at any stage of a mission life cycle can call SPICE APIs to access this data and compute geometric quantities required for observation planning, engineering assessment and science data analysis. SPICE is implemented in three different languages, supported on 20+ computer environments, and distributed with complete source code and documentation. It includes capabilities that are extensively tested by everyday use in many active projects and are applicable to all types of space missions - flyby, orbiters, observatories, landers and rovers. While a customer's initial SPICE adaptation for the first mission or experiment requires a modest effort, this initial effort pays off because adaptation for subsequent missions/experiments is just a small fraction of the initial investment, with the majority of tools based on SPICE requiring no or very minor changes.

  5. Participatory learning and knowledge assessment with a game-based method relying on student-generated questions

    CERN Document Server

    Abad, Enrique; Gil, Julia

    2015-01-01

    A game based on student-generated multiple-choice questions (MCQs) was used to promote participatory learning and as a knowledge assessment tool in the framework of an elementary course in Photonics. Under the instructor's guidance, students were asked to author MCQs, including both question stems and four possible answers (three distractors and one correct answer). They were told that good enough questions would enter a repository from which MCQs for the final exam could be drawn. The student-generated MCQs were then reviewed by the instructor, who discarded unsuitable questions and made amendments to ensure quality standards. The resulting repository (with the key to the correct answers) was made available to the students, whereupon a subset of questions were selected by the instructor to set the MCQ test for the final exam (consisting of a MCQ test based on student-generated questions and a problem-solving part set entirely by the instructor). The MCQ repository was large enough to ensure that rote learnin...

  6. Proximal fibular stress fractures in children and adolescents, what should we rely on? Lessons learned from a case.

    Science.gov (United States)

    de Pina, Carlos Aguiar Ramos; Balacó, Inês; Serrano, Pedro Ruas; Matos, Gabriel

    2015-02-16

    Fibula fractures are the third most common stress fractures in children and adolescents. The triad of localised periosteal reaction, endosteal thickening and radiolucent cortical lines, localised in the distal third of the fibula along with a typical clinical history is in most cases sufficient to establish the diagnosis. Proximal fibula stress lesions are a very rare finding, with few reports in the literature. Stress fractures in this location demand careful investigation before a definitive diagnosis can be made. In the presence of non-specific MRI findings, stress fracture should be a diagnosis of exclusion. The authors report a case referred to their department by a sports medicine physician with a suspicion of stress fracture of the proximal fibula without characteristic imaging findings, which was ultimately confirmed as a stress lesion only by biopsy.

  7. 12 CFR 221.117 - When bank in “good faith” has not relied on stock as collateral.

    Science.gov (United States)

    2010-01-01

    ... the margin stock as collateral,” contained in paragraph (2)(iv) of the definition of indirectly... because of fluctuations in market value of the stock, but instead was payable on one or more fixed... stock as collateral. 221.117 Section 221.117 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED)...

  8. Enterocyte loss of polarity and gut wound healing rely upon the F-actin-severing function of villin.

    Science.gov (United States)

    Ubelmann, Florent; Chamaillard, Mathias; El-Marjou, Fatima; Simon, Anthony; Netter, Jeanne; Vignjevic, Danijela; Nichols, Buford L; Quezada-Calvillo, Roberto; Grandjean, Teddy; Louvard, Daniel; Revenu, Céline; Robine, Sylvie

    2013-04-09

    Efficient wound healing is required to maintain the integrity of the intestinal epithelial barrier because of its constant exposure to a large variety of environmental stresses. This process implies a partial cell depolarization and the acquisition of a motile phenotype that involves rearrangements of the actin cytoskeleton. Here we address how polarized enterocytes harboring actin-rich apical microvilli undergo extensive cell remodeling to drive injury repair. Using live imaging technologies, we demonstrate that enterocytes in vitro and in vivo rapidly depolarize their microvilli at the wound edge. Through its F-actin-severing activity, the microvillar actin-binding protein villin drives both apical microvilli disassembly in vitro and in vivo and promotes lamellipodial extension. Photoactivation experiments indicate that microvillar actin is mobilized at the lamellipodium, allowing optimal migration. Finally, efficient repair of colonic mechanical injuries requires villin severing of F-actin, emphasizing the importance of villin function in intestinal homeostasis. Thus, villin severs F-actin to ensure microvillus depolarization and enterocyte remodeling upon injury. This work highlights the importance of specialized apical pole disassembly for the repolarization of epithelial cells initiating migration.

  9. The walking-induced transient hack concept is valid & relies on a transient early-exercise hypoxemia.

    Directory of Open Access Journals (Sweden)

    Antoine Bruneau

    Full Text Available BACKGROUND: Decreased arterial oxygen pressure obtained at peak exercise is strong evidence of walking-induced hypoxemia, assuming that the lower pressure occurs just before exercise is stopped. Using empirical predefined models and transcutaneous oximetry, we have shown that some patients reporting exercise intolerance show a minimal value at the onset of walking and a post-exercise overshoot. These changes are referred to as transcutaneous "walking-induced transient hacks". METHODS: In 245 patients, walking-induced transcutaneous oxygen pressure changes in the chest were analyzed using observer-independent clustering techniques. Clustering classes were compared to the profile types previously proposed with the cross-correlation technique. The classifications of patients according to both approaches were compared using kappa statistics. In 10 patients showing a hack on transcutaneous oximetry, we analyzed the results of direct iterative arterial sampling recorded during a new walking treadmill test. RESULTS: Clustering analysis resulted in 4 classes that closely fit the 4 most frequently proposed empirical models (cross-correlation coefficients: 0.93 to 0.97. The kappa between the two classifications was 0.865. In 10 patients showing transcutaneous hacks, the minimal direct arterial oxygen pressure value occurred at exercise onset, and these patients exhibited a recovery overshoot reaching a maximum at two minutes of recovery, confirming the walking-induced transient hypoxemia. CONCLUSIONS: In patients reporting exercise intolerance, transcutaneous oximetry could help to detect walking-induced transient hypoxemia, while peak-exercise arterial oximetry might be normal.

  10. Evaluation Techniques of Creating Coherence in Poems of Kaiser Aminpour Relying on the Theory of Halliday’s Linguistics

    Directory of Open Access Journals (Sweden)

    Reza Sattari

    2016-05-01

    Full Text Available AbstractOne of the linguistics theories that are utilized in present age in the analysis of literary texts is the theory of Halliday’s linguistics which is called theory of systemic - functional linguistics. Halliday’s theory in the second half of the twentieth century, in contrast to the "generative linguistics" approach, which is based on formal language like a mental and individual phenomenon.    Hallidayan called the semantic, literal, syntax and logical relationship of words as coherence of a text and believes that the coherence of the text, includes all the semantic relations by which, every piece of speech or writing can serve as text. Hence, cohesion, referred to relations that relates all elements of a sentence to the elements of other sentences. From Hallidayan's  point of view, elements of coherence of a text are divided into two parts: grammatical and lexical. Grammatical cohesion includes factors such as referrals, replacements, removals and relevancy, and lexical coherence includes repetition and collocation in literary language.   Kaiser is one of the contemporary famous poets that have composed many poets in different molds of poetry. Kaiser Manipur's poems benefit the integration due to the poet's fluency in Persian and his familiarity with the vocabulary and grammatical and lexical capacities of Persian literature. In this research, we have studied the cohesion in Kaiser Manipur's poems from two views of lexical and grammatical by using Holidaying theory of coherence as well as the techniques that the poet has applied to reach the cohesion in his poems.    The method of research in the present paper is descriptive-analytical and all the information and data has been collected from the sources of library and electronic. In this paper, we first briefly discussed the Holidaying coherence theory, and then, with a special approach to this theory, the most important techniques that Kaiser Manipur has benefited to create the coherence in his poems and poetry, analyzed and investigated.   The Research results indicate that the poems of Kaiser Manipur have a specific integration due to the poet's fluency in Persian and his familiarity with the vocabulary and grammatical and lexical capacities of Persian literature. Hence, techniques of creating coherence in two aspects of grammatical cohesion and lexical cohesion in Kaiser Manipur's poetry are considerable. Grammatical coherence in his poems is achieved through techniques such as references, omission, and relevance. And the techniques of lexica coherence in his poems are: using phonemes, repeating the words, pun, repeated sentence, the balance, the heart of the matter, proportion, contrast, sequence and so on.    Kaiser Aminpour has widely used the techniques of lexical and grammatical coherence in his poems, so that in most of his poems we can see several techniques used together in a way to reach the mentioned coherence. Hence, we can say he is a poet who knows his language as well as words and has the complete power of selection and combination of words and sentences. And his poems are very coherent and the sentences and phrases are intertwined and related to each other in his poetry.Keywords: Kaiser Aminpour, Hallidayan, functional linguistics, grammatical cohesion, lexical cohesion.References 1.      Abdollahi, Manije (2005. Vertical link and semantic cohesion in the ghazals of Hafez. Journal of Humanities and Social Sciences, University of Shiraz, (Vol. 22. No 3(44. P.p: 124- 134. [In Persian].2.      Afrashi, Azita (1999. A look to the issue of collocation of words. Language and Literature, No 7 & 8. p.p: 73- 83. [In Persian].3.      Aghahosseini, H. & Gholami, Mojahed. (2010. Indian poetry style aesthetic effects on the resistance poetry of Kaiser Aminpour's poetry. Journal of resistance literature at Kerman Shahid Bahonar University. Year, 1. No, 2. P.p: 1- 29. [In Persian]. 4.      Ahmadzadeh, Mostafa. (2008. Linguistic interpretation of Surah Al-Asr. Islamic Studies: Quran and Hadith Sciences. Year 40. No, 3/18. P.p: 11-35. [In Persian].5.       Alavi-moghaddam, M. (1998. Contemporary literary criticism theories (formalism and structuralism. Tehran: Samt. [In Persian].6.      Aminpour, Kaisar (2011. Complete collection of kaisar Aminpour's poems. 8th ed., Tehran: Morvarid. [In Persian].Abdollahi, M. (2005.  7.      Behjo, Zohre (1998. Encoding scheme of methods of coherence in Persian language. Farhang, No 25 & 26. P.p: 189- 212. [In Persian].8.      Fotohi, M. (2008. Three voices, three colors, three styles of Aminpour’s poem. Literature Studies, University of Guilan. No 5. P.p: 9-30. [In Persian].9.      Ghobadi, H. & Rezaei-Jamkarani, A. (2011. Review, analysis and criticism of four Persian odes based on the teachings of systemic-functional linguistics. Journal of Persian Language and Literature. No 20. P.p: 69- 93. [In Persian].10.  Gholamhosseinzade, GH. & Norouzi, H. (2010.  Role of Phonetic repeating in lexical Cohesion in prosodic Persian poetry. Journal of Literature and Language University of Kerman. No, 27. P.p: 251- 281. [In Persian].11.  Halliday, M. A. K & Hasan, R. (1976 Cohesion in English. London: Longman, [In English].12.  Halliday, M. A. K. (1985. An Introduction to Functional Grammar. London: Edward Arnold. [In English].13.  Jahangiri, N. & Zakipour, Sh. (2005. Lexical cohesion in Persian short stories for children. Literary Notes (Journal of Ferdowsi University of Mashhad. No 151. P.p: 1-20. [In Persian].14.  Khanjan, A. & Mirza, Z. (2006. Introduction to the functional poetry (linguistic approaches. Journal of Persian Language and Literature. No 7. P.p: 83- 101. [In Persian].15.  Lotfipour-saedi, K. (1992. An introduction to the principles and methods of translation. Tehran: Academic publishing. [In Persian].16.  Martine, A. (2001. Phonetic transformation balance. (H. Milanian Trans. Terhan: Hermes. [In Persian].17.  Moein od-Dini, F. (2003.  Techniques to create coherence in kalila and demna. Farhang, No 47 & 47. P.p: 303- 326. [In Persian].18.  Mohajer, M. & Nabavi, M. (1997. Towards the linguistic poetry: the functional approach. Tehran: Markaz. [In Persian].19.  Natel Khanlari, P. (2007. Persian poetry weight. 7th ed., Tehran: toos. [In Persian].20.  Panahi, S. (2002. The process of collocation and collocation compounds in Persian language. Journal of Academy. No19. P.p: 199- 211. [In Persian].21.  Rastgoo, M. (2004. Speak art layout (art of badie. Tehran: Samt. [In Persian].22.  Rouhani, M. (2011.  Study of Repeats' Functions in Contemporary Poetry (based on poetry of Sepehri, Shamloo and Forough. Journal of Boostan Adab (university of Shiraz. Year 3. No 2/8. P.p: 145- 168. [In Persian].23.  Safavi, K. (2008. Introduction to Semantics. 3rd ed., Tehran: Sore-ye Mehr. [In Persian]. 24.  Safavi, K. (1994. From linguistics to literature. (Vol: 1. Tehran: Cheshme. [In Persian].25.  Salehi, F. (2007.  Semantics and functional grammar of Halliday. Literature Book of the Month. No 8. P.p: 32-41. [In Persian].26.  Sarli, N. & Ishani, T. (2011.  Coherence and Coordination of cohesion Theory and its Application in a Minimal Story Persian (The Story of the Ladder. Journal of Language Scholarships (university of az-Zahra. Year 2. No 4. P.p: 51- 77. [In Persian].27.  Shabanloo, A. & Maleksabet, M. & Jalali, Y. (2008.  Grammatical cohesion process in the long poem of am'aq Bukhari. Journal of Gawhar-i Guya. No 5. P.p: 165- 188. [In Persian].28.  Shafi’ee kadkani, M. (2005. Music of Lyrics. (8th ed., Tehran: Agah. [In Persian].29.  Shamisa, S. (2007. Figures of speech: a new look to Ba'die. 2nd ed., Tehran: Mitra. [In Persian].30.  Vafaei, A. & Alinoori, Z. (2010. Artistic symmetry in two axes of succession and Companion in poems of Kaiser Aminpour. Journal of Persian Language and Literature. Year 2. No 6. P: 99- 118. [In Persian].31.  Velek, R. & Varen, A. (2003. Theory of Literatur. (Z. Movahhed & P. Mohajer Trans. Tehran: Elmi- Farhangi. [In Persian].Yahaghi, M. & Fallahi, M. (2010.  Saadi's and Bidel Dehlavi's sonnets text cohesion, evaluation and comparison of ten sonnets of Sa'di, and ten sonnets of Bidel. Journal of Literature and Language University of Kerman. No 27. P: 327- 346. [In Persian].

  11. Nocturnal Foraging by Red-Legged Kittiwakes, a Surface Feeding Seabird That Relies on Deep Water Prey During Reproduction.

    Science.gov (United States)

    Kokubun, Nobuo; Yamamoto, Takashi; Kikuchi, Dale M; Kitaysky, Alexander; Takahashi, Akinori

    2015-01-01

    Narrow foraging specialization may increase the vulnerability of marine predators to climate change. The red-legged kittiwake (Rissa brevirostris) is endemic to the Bering Sea and has experienced drastic population fluctuations in recent decades, presumably due to climate-driven changes in food resources. Red-legged kittiwakes are presumed to be a nocturnal surface-foraging seabird that feed almost entirely on deep water Myctophidae fishes. However, there is little empirical evidence confirming their nocturnal foraging activity during the breeding season. This study investigated the foraging behavior of red-legged kittiwakes by combining GPS tracking, accelerometry, and dietary analyses at the world's largest breeding colony of red-legged kittiwakes on St. George I. GPS tracking of 5 individuals revealed that 82.5% of non-flight behavior (including foraging and resting) occurred over the ocean basin (bottom depth >1,000 m). Acceleration data from 4 birds showed three types of behaviors during foraging trips: (1) flight, characterized by regular wing flapping, (2) resting on water, characterized by non-active behavior, and (3) foraging, when wing flapping was irregular. The proportions of both foraging and resting behaviors were higher at night (14.1 ± 7.1% and 20.8 ± 14.3%) compared to those during the day (6.5 ± 3.0% and 1.7 ± 2.7%). The mean duration of foraging (2.4 ± 2.9 min) was shorter than that of flight between prey patches (24.2 ± 53.1 min). Dietary analyses confirmed myctophids as the dominant prey (100% by occurrence and 98.4 ± 2.4% by wet-weight). Although the sample size was limited, these results suggest that breeding red-legged kittiwakes concentrated their foraging on myctophids available at the surface during nighttime in deep water regions. We propose that the diel patterns and ephemeral nature of their foraging activity reflected the availability of myctophids. Such foraging specialization may exacerbate the vulnerability of red-legged kittiwakes to climate change in the Bering Sea.

  12. Thiol-Based Peroxidases and Ascorbate Peroxidases: Why Plants Rely on Multiple Peroxidase Systems in the Photosynthesizing Chloroplast?

    Science.gov (United States)

    Dietz, Karl-Josef

    2016-01-01

    Photosynthesis is a highly robust process allowing for rapid adjustment to changing environmental conditions. The efficient acclimation depends on balanced redox metabolism and control of reactive oxygen species release which triggers signaling cascades and potentially detrimental oxidation reactions. Thiol peroxidases of the peroxiredoxin and glutathione peroxidase type, and ascorbate peroxidases are the main peroxide detoxifying enzymes of the chloroplast. They use different electron donors and are linked to distinct redox networks. In addition, the peroxiredoxins serve functions in redox regulation and retrograde signaling. The complexity of plastid peroxidases is discussed in context of suborganellar localization, substrate preference, metabolic coupling, protein abundance, activity regulation, interactions, signaling functions, and the conditional requirement for high antioxidant capacity. Thus the review provides an opinion on the advantage of linking detoxification of peroxides to different enzymatic systems and implementing mechanisms for their inactivation to enforce signal propagation within and from the chloroplast.

  13. Relying on satiety cues in food consumption : studies on the role of social context, appearance focus, and mindfulness

    NARCIS (Netherlands)

    Veer, van de E.

    2013-01-01

    Dit proefschrift richt zich op de vraag hoe een consumptiemoment de hoeveelheid consumptie op een later moment kan beïnvloeden. Gedurende de dag eten consumenten op verschillende momenten. Specifiek richt dit proefschrift zich op de vraag hoe de sociale context van een consumptie-episode latere cons

  14. Embracing Advancement: You Can't Rely on Outside Funding. How to Build a Strong Internal Fundraising Machine

    Science.gov (United States)

    Heaton, Paul

    2014-01-01

    In the past 7 years of working at and in community colleges, the author has seen two distinct trends: (1) Community colleges appear to be ushering in a new era of advancement; and (2) Community colleges themselves are often the biggest impediment to successful advancement efforts. Despite millions of grateful learners and business and community…

  15. How to Quickly Cast off Poverty in Poverty-stricken Mountainous Areas Relying on the "Whole Village Advancement" Model?

    Institute of Scientific and Technical Information of China (English)

    Zhongna; YANG; Jijun; TANG; Xiaoling; YU

    2013-01-01

    We carry out a field survey on the implementation of the"Whole Village Advancement"poverty alleviation model in Lisizhuang Village,Shunping County,Hebei Province.Based on the"Whole Village Advancement"poverty alleviation model,we put forth the following recommendations:(i)Oriented by the market,fostering the function of"blood-refreshing";(ii)Strengthening the new ideas of using projects to attract investment;(iii)Implementing the new strategy of"driven by the able person";(iv)Improving the farmers’quality;(v)Adhering to the diversification of financing channels.

  16. Tonoplast targeting of VHA-a3 relies on a Rab5-mediated but Rab7-independent vacuolar trafficking route.

    Science.gov (United States)

    Feng, Qiang-Nan; Zhang, Yan; Li, Sha

    2017-04-01

    Vacuolar trafficking routes and their regulators have recently drawn lots of attention in plant cell biology. A recent study reported the discovery of a plant-specific vacuolar trafficking route, i.e., a direct ER-to-vacuole route, through analysis of VHA-a3 subcellular targeting, a key component for the tonoplast V-ATPases. Our recent findings showed that VHA-a3 targets to the tonoplast through a Rab5-mediated but Rab7-independent pathway, shedding new lights on the unconventional vacuolar trafficking route in plant cells. © 2017 Institute of Botany, Chinese Academy of Sciences.

  17. Motor learning relies on integrated sensory inputs in ADHD, but over-selectively on proprioception in autism spectrum conditions.

    Science.gov (United States)

    Izawa, Jun; Pekny, Sarah E; Marko, Mollie K; Haswell, Courtney C; Shadmehr, Reza; Mostofsky, Stewart H

    2012-04-01

    The brain builds an association between action and sensory feedback to predict the sensory consequence of self-generated motor commands. This internal model of action is central to our ability to adapt movements and may also play a role in our ability to learn from observing others. Recently, we reported that the spatial generalization patterns that accompany adaptation of reaching movements were distinct in children with autism spectrum disorder (ASD) as compared with typically developing (TD) children. To test whether the generalization patterns are specific to ASD, here, we compared the patterns of adaptation with those in children with attention deficit hyperactivity disorder (ADHD). Consistent with our previous observations, we found that in ASD, the motor memory showed greater than normal generalization in proprioceptive coordinates compared with both TD children and children with ADHD; children with ASD also showed slower rates of adaptation compared with both control groups. Children with ADHD did not show this excessive generalization to the proprioceptive target, but they did show excessive variability in the speed of movements with an increase in the exponential distribution of responses (τ) as compared with both TD children and children with ASD. The results suggest that slower rate of adaptation and anomalous bias towards proprioceptive feedback during motor learning are characteristics of autism, whereas increased variability in execution is a characteristic of ADHD.

  18. One of Brazil's Largest Clothing Manufacturers Relies on Gerber Equipment to Support Aggresive Growth Strategy

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Gerber Technology announces that one of Brazil's largest and most recognized apparel companies,Cia.Hering,has chosen Gerber to expand its facilities in the state of Santa Catarina and equip its newest factories in the states of Goias and Rio Grande do Norte with design and manufacturing solutions valued at $1.4 million.

  19. Critical appraisal of quantitative PCR results in colorectal cancer research: Can we rely on published qPCR results?

    NARCIS (Netherlands)

    Dijkstra, J.R.; Kempen, L.C.L.T. van; Nagtegaal, I.D.; Bustin, S.A.

    2014-01-01

    The use of real-time quantitative polymerase chain reaction (qPCR) in cancer research has become ubiquitous. The relative simplicity of qPCR experiments, which deliver fast and cost-effective results, means that each year an increasing number of papers utilizing this technique are being published. B

  20. Critical appraisal of quantitative PCR results in colorectal cancer research: can we rely on published qPCR results?

    Science.gov (United States)

    Dijkstra, J R; van Kempen, L C; Nagtegaal, I D; Bustin, S A

    2014-06-01

    The use of real-time quantitative polymerase chain reaction (qPCR) in cancer research has become ubiquitous. The relative simplicity of qPCR experiments, which deliver fast and cost-effective results, means that each year an increasing number of papers utilizing this technique are being published. But how reliable are the published results? Since the validity of gene expression data is greatly dependent on appropriate normalisation to compensate for sample-to-sample and run-to-run variation, we have evaluated the adequacy of normalisation procedures in qPCR-based experiments. Consequently, we assessed all colorectal cancer publications that made use of qPCR from 2006 until August 2013 for the number of reference genes used and whether they had been validated. Using even these minimal evaluation criteria, the validity of only three percent (6/179) of the publications can be adequately assessed. We describe common errors, and conclude that the current state of reporting on qPCR in colorectal cancer research is disquieting. Extrapolated to the study of cancer in general, it is clear that the majority of studies using qPCR cannot be reliably assessed and that at best, the results of these studies may or may not be valid and at worst, pervasive incorrect normalisation is resulting in the wholesale publication of incorrect conclusions. This survey demonstrates that the existence of guidelines, such as MIQE, is necessary but not sufficient to address this problem and suggests that the scientific community should examine its responsibility and be aware of the implications of these findings for current and future research.

  1. Spleen-Preserving Distal Pancreatectomy with Resection of the Splenic Vessels. Should One Rely on the Short Gastric Arteries?

    Directory of Open Access Journals (Sweden)

    Vyacheslav Ivanovich Egorov

    2011-09-01

    Full Text Available Context Knowing the collaterals is essential for a spleen-preserving distal pancreatectomy with resection of the splenic vessels. Objective To ascertain the sources of the blood supply to the spleen after a spleen-preserving distal pancreatectomy with resection of the splenic vessels. Methods Perfusion of the cadaveric left gastric and right gastroepiploic arteries with methylene blue after occlusion of all the arteries except the short gastric arteries (n=10. Intraoperative color Doppler ultrasound was used for the evaluation of the hilar arterial blood flow at distal pancreatectomy (n=23 after 1 clamping of the splenic artery alone, 2 clamping of the splenic and left gastroepiploic arteries and 3 clamping of the splenic and short gastric arteries. CT angiography of the gastric and splenic vessels before and after a spleen-preserving distal pancreatectomy (n=10. Results Perfusion of the cadaveric arteries revealed no effective direct or indirect (through the submucous gastric arterial network communication between the left gastric and the branches of the short gastric arteries. In no case did intraoperative color Doppler ultrasound detect any hilar arterial blood flow after the clamping of the splenic and left gastroepiploic arteries. The clamping of the short gastric arteries did not change the flow parameters. In none of the cases did a post-spleen-preserving distal pancreatectomy with resection of the splenic vessels CT angiography delineate the short gastric vessels supplying the spleen. In all cases, the gastroepiploic arcade was the main arterial pathway feeding the spleen. Conclusion Experimental, intra- and postoperative instrumental investigations did not show the short gastric arteries to be engaged in the blood supply to the spleen after a spleen-preserving distal pancreatectomy with resection of the splenic vessels. In all cases, the left gastroepiploic artery was the main collateral vessel.

  2. IL-7-Induced Proliferation of Human Naive CD4 T-Cells Relies on Continued Thymic Activity

    Science.gov (United States)

    Silva, Susana L.; Albuquerque, Adriana S.; Matoso, Paula; Charmeteau-de-Muylder, Bénédicte; Cheynier, Rémi; Ligeiro, Dário; Abecasis, Miguel; Anjos, Rui; Barata, João T.; Victorino, Rui M. M.; Sousa, Ana E.

    2017-01-01

    Naive CD4 T-cell maintenance is critical for immune competence. We investigated here the fine-tuning of homeostatic mechanisms of the naive compartment to counteract the loss of de novo CD4 T-cell generation. Adults thymectomized in early childhood during corrective cardiac surgery were grouped based on presence or absence of thymopoiesis and compared with age-matched controls. We found that the preservation of the CD31− subset was independent of the thymus and that its size is tightly controlled by peripheral mechanisms, including prolonged cell survival as attested by Bcl-2 levels. Conversely, a significant contraction of the CD31+ naive subset was observed in the absence of thymic activity. This was associated with impaired responses of purified naive CD4 T-cells to IL-7, namely, in vitro proliferation and upregulation of CD31 expression, which likely potentiated the decline in recent thymic emigrants. Additionally, we found no apparent constraint in the differentiation of naive cells into the memory compartment in individuals completely lacking thymic activity despite upregulation of DUSP6, a phosphatase associated with increased TCR threshold. Of note, thymectomized individuals featuring some degree of thymopoiesis were able to preserve the size and diversity of the naive CD4 compartment, further arguing against complete thymectomy in infancy. Overall, our data suggest that robust peripheral mechanisms ensure the homeostasis of CD31− naive CD4 pool and point to the requirement of continuous thymic activity to the maintenance of IL-7-driven homeostatic proliferation of CD31+ naive CD4 T-cells, which is essential to secure T-cell diversity throughout life. PMID:28154568

  3. Vaginal dose assessment in image-guided brachytherapy for cervical cancer: Can we really rely on dose-point evaluation?

    Science.gov (United States)

    Limkin, Elaine Johanna; Dumas, Isabelle; Rivin Del Campo, Eleonor; Chargari, Cyrus; Maroun, Pierre; Annède, Pierre; Petit, Claire; Seisen, Thomas; Doyeux, Kaya; Tailleur, Anne; Martinetti, Florent; Lefkopoulos, Dimitri; Haie-Meder, Christine; Mazeron, Renaud

    2016-01-01

    Although dose-volume parameters in image-guided brachytherapy have become a standard, the use of posterior-inferior border of the pubic symphysis (PIBS) points has been recently proposed in the reporting of vaginal doses. The aim was to evaluate their pertinence. Nineteen patients who received image-guided brachytherapy after concurrent radiochemotherapy were included. Per treatment, CT scans were performed at Days 2 and 3, with reporting of the initial dwell positions and times. Doses delivered to the PIBS points were evaluated on each plan, considering that they were representative of one-third of the treatment. The movements of the applicator according to the PIBS point were analysed. Mean prescribed doses at PIBS -2, PIBS, PIBS +2 were, respectively, 2.23 ± 1.4, 6.39 ± 6.6, and 31.85 ± 36.06 Gy. Significant differences were observed between the 5 patients with vaginal involvement and the remaining 14 at the level of PIBS +2 and PIBS: +47.60 Gy and +7.46 Gy, respectively (p = 0.023 and 0.03). The variations between delivered and prescribed doses at PIBS points were not significant. However, at International commission on radiation units and measurements rectovaginal point, the delivered dose was decreased by 1.43 ± 2.49 Gy from the planned dose (p = 0.019). The delivered doses at the four points were strongly correlated with the prescribed doses with R(2) ranging from 0.93 to 0.95. The movements of the applicator in regard of the PIBS point assessed with the Digital Imaging and Communications in Medicine coordinates were insignificant. The doses evaluated at PIBS points are not impacted by intrafractional movements. PIBS and PIBS +2 dose points allow distinguishing the plans of patients with vaginal infiltration. Further studies are needed to correlate these parameters with vaginal morbidity. Copyright © 2016 American Brachytherapy Society. Published by Elsevier Inc. All rights reserved.

  4. So you want to work? What employers say about job skills, recruitment and hiring employees who rely on AAC.

    Science.gov (United States)

    Bryen, Diane Nelson; Potts, Blyden B; Carey, Allison C

    2007-06-01

    In order to better understand employer perspectives with respect to hiring and working with people who use AAC and the kinds of employment barriers they believe exist for these individuals, a study into the job requirements, recruitment processes, and hiring processes of 27 employers in the United States was conducted. Interviews with the 27 employers focused on 48 jobs in 25 unique categories, many of which had previously been identified as desirable by 38 adults who used AAC (some of whom were employed and some of whom were seeking employment). Findings suggest that, while level of education is still important to employers, skills such as time management, problem solving, communication, use of an understandable and standard voice, and basic technology may be even more so. The study also found that having an effective job-related network is important, as is the ability to provide credible references and do well during in-person interviews with potential employers.

  5. Autoimmunity conferred by chs3-2D relies on CSA1, its adjacent TNL-encoding neighbour.

    Science.gov (United States)

    Xu, Fang; Zhu, Chipan; Cevik, Volkan; Johnson, Kaeli; Liu, Yanan; Sohn, Kee; Jones, Jonathan D; Holub, Eric B; Li, Xin

    2015-03-05

    Plant innate immunity depends on the function of a large number of intracellular immune receptor proteins, the majority of which are structurally similar to mammalian nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) proteins. CHILLING SENSITIVE 3 (CHS3) encodes an atypical Toll/Interleukin 1 Receptor (TIR)-type NLR protein with an additional Lin-11, Isl-1 and Mec-3 (LIM) domain at its C-terminus. The gain-of-function mutant allele chs3-2D exhibits severe dwarfism and constitutively activated defense responses, including enhanced resistance to virulent pathogens, high defence marker gene expression, and salicylic acid accumulation. To search for novel regulators involved in CHS3-mediated immune signaling, we conducted suppressor screens in the chs3-2D and chs3-2D pad4-1 genetic backgrounds. Alleles of sag101 and eds1-90 were isolated as complete suppressors of chs3-2D, and alleles of sgt1b were isolated as partial suppressors of chs3-2D pad4-1. These mutants suggest that SAG101, EDS1-90, and SGT1b are all positive regulators of CHS3-mediated defense signaling. Additionally, the TIR-type NLR-encoding CSA1 locus located genomically adjacent to CHS3 was found to be fully required for chs3-2D-mediated autoimmunity. CSA1 is located 3.9 kb upstream of CHS3 and is transcribed in the opposite direction. Altogether, these data illustrate the distinct genetic requirements for CHS3-mediated defense signaling.

  6. Enterocyte loss of polarity and gut wound healing rely upon the F-actin–severing function of villin

    Science.gov (United States)

    Ubelmann, Florent; Chamaillard, Mathias; El-Marjou, Fatima; Simon, Anthony; Netter, Jeanne; Vignjevic, Danijela; Nichols, Buford L.; Quezada-Calvillo, Roberto; Grandjean, Teddy; Louvard, Daniel; Revenu, Céline; Robine, Sylvie

    2013-01-01

    Efficient wound healing is required to maintain the integrity of the intestinal epithelial barrier because of its constant exposure to a large variety of environmental stresses. This process implies a partial cell depolarization and the acquisition of a motile phenotype that involves rearrangements of the actin cytoskeleton. Here we address how polarized enterocytes harboring actin-rich apical microvilli undergo extensive cell remodeling to drive injury repair. Using live imaging technologies, we demonstrate that enterocytes in vitro and in vivo rapidly depolarize their microvilli at the wound edge. Through its F-actin–severing activity, the microvillar actin-binding protein villin drives both apical microvilli disassembly in vitro and in vivo and promotes lamellipodial extension. Photoactivation experiments indicate that microvillar actin is mobilized at the lamellipodium, allowing optimal migration. Finally, efficient repair of colonic mechanical injuries requires villin severing of F-actin, emphasizing the importance of villin function in intestinal homeostasis. Thus, villin severs F-actin to ensure microvillus depolarization and enterocyte remodeling upon injury. This work highlights the importance of specialized apical pole disassembly for the repolarization of epithelial cells initiating migration. PMID:23520048

  7. Bridge-induced chromosome translocation in yeast relies upon a Rad54/Rdh54-dependent, Pol32-independent pathway.

    Directory of Open Access Journals (Sweden)

    Valentina Tosato

    Full Text Available While in mammalian cells the genetic determinism of chromosomal translocation remains unclear, the yeast Saccharomyces cerevisiae has become an ideal model system to generate ad hoc translocations and analyze their cellular and molecular outcome. A linear DNA cassette carrying a selectable marker flanked by perfect homologies to two chromosomes triggers a bridge-induced translocation (BIT in budding yeast, with variable efficiency. A postulated two-step process to produce BIT translocants is based on the cooperation between the Homologous Recombination System (HRS and Break-Induced Replication (BIR; however, a clear indication of the molecular factors underlying the genetic mechanism is still missing. In this work we provide evidence that BIT translocation is elicited by the Rad54 helicase and completed by a Pol32-independent replication pathway. Our results demonstrate also that Rdh54 is involved in the stability of the translocants, suggesting a mitotic role in chromosome pairing and segregation. Moreover, when RAD54 is over-expressed, an ensemble of secondary rearrangements between repeated DNA tracts arise after the initial translocation event, leading to severe aneuploidy with loss of genetic material, which prompts the identification of fragile sites within the yeast genome.

  8. Retrieving Targeted Results from a Static File Repository using a Keyword matching Mechanism relying on a Cluster-based Algorithm

    Directory of Open Access Journals (Sweden)

    Madalina ZURINI

    2013-07-01

    Full Text Available Due to the fact that the web based solutions designed for learning contain, among the compulsory functions, the uploading of didactic materials of the person doing the examining (on a regular basis this being the professor and the possibility of accessing these by the examinee (on a regular basis this being the student, within this paper we have chosen to set the goal of finding a resolution that will enable the access of content relevant to the person being examined. Hereby we have suggested a prototype which will capacitate the singling out and grouping of documents depending on the keywords, which will be followed by a visual search depending on the distance between two documents, by the recurrence of the closest k documents to the one being the element of interest - reaching the optimal alternative in case of a performance oriented point of view. The algorithm needed for the extraction of this data is presented within the paper. An optimization model is proposed in order to reduce the time consuming component in regards to the minimization of differences in the quality of the documents resulted in the automatic search using a k Nearest Neighbour grid search engine.

  9. Brain, not brawn : tiny Bruin Instruments relies on innovation to win a global niche for its chemical pumps

    Energy Technology Data Exchange (ETDEWEB)

    Byfield, M.

    2010-06-15

    Bruin Instruments is a chemical pump manufacturer based in Alberta. The company has designed low pressure pumps for shale gas operations and has also developed low volume chemical injection pumps to reduce the use of expensive chemicals in field pumps. The company uses nitriting main rods in their pumps to improve durability, and has added clear plastic lids to their chemical pump mechanisms to allow operators to monitor liquid flow without removing the lid. The company is now investigating methods of applying solar technology to its pumps, and is in the process of developing a controller that combines solar control and regulation functions in a single device. The company's manufacturing and repairs operations now account for 80 per cent of its revenue. Its line of pumps will soon be carried by suppliers in the United States. While the recent economic downturn has led to cutbacks, Bruin Instruments has made a long-term commitment to expanding its international clientele. 2 figs.

  10. How to reduce resistance to movement of alkane liquid drops across tilted surfaces without relying on surface roughening and perfluorination.

    Science.gov (United States)

    Urata, Chihiro; Masheder, Benjamin; Cheng, Dalton F; Hozumi, Atsushi

    2012-12-21

    Alkylsilane-derived monolayer-covered surfaces generally display a reasonably good level of hydrophobicity but poor oleophobicity. Here, we demonstrate that the physical attributes of alkylsilane-derived surfaces (liquid-like or solid-like) are dependent on the alkyl chain length and density, and these factors subsequently have significant influence upon the dynamic dewetting behavior toward alkanes (C(n)H(2n+2), where n = 7-16). In this study, we prepared and characterized hybrid films through a simple sol-gel process based on the cohydrolysis and co-condensation of a mixture of a range of alkyltriethoxysilanes (C(n)H(2n+1)Si(OEt)(3), where n = 3, 6, 8, 10, 12, 14, 16, and 18) and tetramethoxysilane (TMOS). Surprisingly, when the carbon number (C(n)) of alkyl chain was 10 and below, the produced hybrid films were all smooth, highly transparent, and showed negligible contact angle (CA) hysteresis. On these hybrid surfaces, 5 μL drops of alkanes (n-hexadecane, n-dodecane, and n-decane) could move easily at low tilt angles (oleophobicity. Therefore, the critical C(n) of alkyl chain used for determining final dynamic dewetting behavior against alkane liquids was 12. Furthermore, our hybrid surfaces exhibited excellent antifingerprint properties, particularly demonstrating low adhesion and easy removal from the surface.

  11. Restoration of the Cirsio dissecti-Molinietum in The Netherlands : Can we rely on soil seed banks?

    NARCIS (Netherlands)

    Matus, G; Verhagen, R; Bekker, RM; Grootjans, AP; Bruelheide, H.

    2003-01-01

    Vegetation and soil seed banks of a threatened Atlantic fen meadow community were studied using recent phytosociological records and seedling emergence from soil samples. Similarly managed but differently degraded stands that suffered different levels of species impoverishment were compared. The act

  12. A CORF computational model of a simple cell that relies on LGN input outperforms the Gabor function model

    NARCIS (Netherlands)

    Azzopardi, George; Petkov, Nicolai

    Simple cells in primary visual cortex are believed to extract local contour information from a visual scene. The 2D Gabor function (GF) model has gained particular popularity as a computational model of a simple cell. However, it short-cuts the LGN, it cannot reproduce a number of properties of real

  13. Lactoperoxidase folding and catalysis relies on the stabilization of the alpha-helix rich core domain: a thermal unfolding study.

    Science.gov (United States)

    Boscolo, Barbara; Leal, Sónia S; Ghibaudi, Elena M; Gomes, Cláudio M

    2007-09-01

    Lactoperoxidase (LPO) belongs to the mammalian peroxidase family and catalyzes the oxidation of halides, pseudo-halides and a number of aromatic substrates at the expense of hydrogen peroxide. Despite the complex physiological role of LPO and its potential involvement in carcinogenic mechanisms, cystic fibrosis and inflammatory processes, little is known on the folding and structural stability of this protein. We have undertaken an investigation of the conformational dynamics and catalytic properties of LPO during thermal unfolding, using complementary biophysical techniques (differential scanning calorimetry, electron spin resonance, optical absorption, fluorescence and circular dichroism spectroscopies) together with biological activity assays. LPO is a particularly stable protein, capable of maintaining catalysis and structural integrity up to a high temperature, undergoing irreversible unfolding at 70 degrees C. We have observed that the first stages of the thermal denaturation involve a minor conformational change occurring at 40 degrees C, possibly at the level of the protein beta-sheets, which nevertheless does not result in an unfolding transition. Only at higher temperature, the protein hydrophobic core, which is rich in alpha-helices, unfolds with concomitant disruption of the catalytic heme pocket and activity loss. Evidences concerning the stabilizing role of the disulfide bridges and the covalently bound heme cofactor are shown and discussed in the context of understanding the structural stability determinants in a relatively large protein.

  14. Do open-cycle hatcheries relying on tourism conserve sea turtles? Sri Lankan developments and economic-ecological considerations.

    Science.gov (United States)

    Tisdell, Clem; Wilson, Clevo

    2005-04-01

    By combining economic analysis of markets with ecological parameters, this article considers the role that tourism-based sea turtle hatcheries (of an open-cycle type) can play in conserving populations of sea turtles. Background is provided on the nature and development of such hatcheries in Sri Lanka. The modeling facilitates the assessment of the impacts of turtle hatcheries on the conservation of sea turtles and enables the economic and ecological consequences of tourism, based on such hatcheries, to be better appreciated. The results demonstrate that sea turtle hatcheries serving tourists can make a positive contribution to sea turtle conservation, but that their conservation effectiveness depends on the way they are managed. Possible negative effects are also identified. Economic market models are combined with turtle population survival relationships to predict the conservation impact of turtle hatcheries and their consequence for the total economic value obtained from sea turtle populations.

  15. Transcription of the major neurospora crassa microRNA-like small RNAs relies on RNA polymerase III.

    Directory of Open Access Journals (Sweden)

    Qiuying Yang

    Full Text Available Most plant and animal microRNAs (miRNAs are transcribed by RNA polymerase II. We previously discovered miRNA-like small RNAs (milRNAs in the filamentous fungus Neurospora crassa and uncovered at least four different pathways for milRNA production. To understand the evolutionary origin of milRNAs, we determined the roles of polymerases II and III (Pol II and Pol III in milRNA transcription. Our results show that Pol III is responsible for the transcription of the major milRNAs produced in this organism. The inhibition of Pol III activity by an inhibitor or by gene silencing abolishes the production of most abundant milRNAs and pri-milRNAs. In addition, Pol III associates with these milRNA producing loci. Even though silencing of Pol II does not affect the synthesis of the most abundant milRNAs, Pol II or both Pol II and Pol III are associated with some milRNA-producing loci, suggesting a regulatory interaction between the two polymerases for some milRNA transcription. Furthermore, we show that one of the Pol III-transcribed milRNAs is derived from a tRNA precursor, and its biogenesis requires RNase Z, which cleaves the tRNA moiety to generate pre-milRNA. Our study identifies the transcriptional machinery responsible for the synthesis of fungal milRNAs and sheds light on the evolutionary origin of eukaryotic small RNAs.

  16. Immigrant and Refugee Students across "Receiving" Nations: To What Extent Can Educators Rely on PISA for Answers?

    Science.gov (United States)

    Rubinstein-Avila, Eliane

    2016-01-01

    Massive population shift is a current global reality--especially given some of the latest development on European shores; some are calling it a humanitarian crisis. Although the United States (US) receives a large number of immigrants (documented and not) and about 70,000 refugees each year, it is certainly not the only nation to do so.…

  17. Postprandial insulin action relies on meal composition and hepatic parasympathetics: dependency on glucose and amino acids: Meal, parasympathetics & insulin action.

    Science.gov (United States)

    Afonso, Ricardo A; Gaspar, Joana M; Lamarão, Iva; Lautt, W Wayne; Macedo, M Paula

    2016-01-01

    Insulin sensitivity (IS) increases following a meal. Meal composition affects postprandial glucose disposal but still remains unclear which nutrients and mechanisms are involved. We hypothesized that gut-absorbed glucose and amino acids stimulate hepatic parasympathetic nerves, potentiating insulin action. Male Sprague-Dawley rats were 24 h fasted and anesthetized. Two series of experiments were performed. (A) IS was assessed before and after liquid test meal administration (10 ml.kg(-1), intraenteric): glucose + amino acids + lipids (GAL, n=6); glucose (n=5); amino acids (n=5); lipids (n=3); glucose + amino acids (GA, n=9); amino acids + lipids (n=3); and glucose + lipids (n=4). (B) Separately, fasted animals were submitted to hepatic parasympathetic denervation (DEN); IS was assessed before and after GAL (n=4) or GA administration (n=4). (A) Both GAL and GA induced significant insulin sensitization. GAL increased IS from 97.9±6.2 mg glucose/kg bw (fasting) to 225.4±18.3 mg glucose/kg bw (Pamino acids trigger a vagal reflex that involves hepatic parasympathetic nerves.

  18. Evaluation of Isolation Methods for Circulating Tumor Cells (CTCs

    Directory of Open Access Journals (Sweden)

    Galatea Kallergi

    2016-11-01

    Full Text Available Background: Detection of CTCs is a poor prognostic factor for many cancer types; however, their very low frequency represents an obstacle for their detection. The objective of the current study was to compare the performance of commonly used methods for CTCs isolation. Methods: The evaluated methods using spiking experiments of MCF7, SKBR3 and MDA MB-231 breast cancer cell lines were (i ficoll density gradient separation (DGS, (ii red blood cell lysis (Erythrolysis isolation, (iii positive immunomagnetic selection (EpCAM Dynal beads, (iv two different negative immunomagnetic separation systems (Dynal vs Miltenyi CD45 beads as well as (v the Cell Search platform and (vi the ISET system. Results: The recovery rates of Erythrolysis and DGS were 39% and 24%, respectively. Magnetic isolations are ranked from the worse to the best recovery rate as follows:, Myltenyi-anti-CD45 microbeads (24%; Dynal-anti-EpCAM beads (75%; Dynabeads-anti-CD45 (97%. CTCs isolation from blood samples using the CellSearch and ISET systems revealed that the recovery rate for Cell Search and ISET was 52% and 95%, respectively. Conclusions: Dynal-anti-CD45 beads have the best recovery rate compared to other magnetic methods. Furthermore the recovery rate of ISET was higher compared to Cell Search, especially for the more aggressive MDA-MB 231 cell line.

  19. Multisite evaluation of point of care CD4 testing in Papua New Guinea.

    Directory of Open Access Journals (Sweden)

    Malin Malagun

    Full Text Available Laboratory-based CD4 monitoring of HIV patients presents challenges in resource limited settings (RLS including frequent machine breakdown, poor engineering support and limited cold chain and specimen transport logistics. This study assessed the performance of two CD4 tests designed for use in RLS; the Dynal assay and the Alere PIMA test (PIMA. Accuracy of Dynal and PIMA using venous blood was assessed in a centralised laboratory by comparison to BD FACSCount (BD FACS. Dynal had a mean bias of -50.35 cells/µl (r(2 = 0.973, p<0.0001, n = 101 and PIMA -22.43 cells/µl (r(2= 0.964, p<0.0001, n = 139 compared to BD FACS. Similar results were observed for PIMA operated by clinicians in one urban (n = 117 and two rural clinics (n = 98. Using internal control beads, PIMA precision was 10.34% CV (low bead mean 214.24 cells/µl and 8.29% (high bead mean 920.73 cells/µl and similar %CV results were observed external quality assurance (EQA and replicate patient samples. Dynal did not perform using EQA and no internal controls are supplied by the manufacturer, however duplicate testing of samples resulted in r(2 = 0.961, p<0.0001, mean bias =  -1.44 cells/µl. Using the cut-off of 350 cells/µl compared to BD FACS, PIMA had a sensitivity of 88.85% and specificity of 98.71% and Dynal 88.61% and 100%. A total of 0.44% (2/452 of patient samples were misclassified as "no treat" and 7.30% (33/452 "treat" using PIMA whereas with Dynal 8.91% (9/101 as "treat" and 0% as "no treat". In our setting PIMA was found to be accurate, precise and user-friendly in both laboratory and clinic settings. Dynal performed well in initial centralized laboratory evaluation, however lacks requisite quality control measures, and was technically more difficult to use, making it less suitable for use at lower tiered laboratories.

  20. Multisite evaluation of point of care CD4 testing in Papua New Guinea.

    Science.gov (United States)

    Malagun, Malin; Nano, Gideon; Chevallier, Caroline; Opina, Ragagalo; Sawiya, Gola; Kivavia, Joseph; Kalinoe, Albina; Nathaniel, Kathalina; Kaminiel, Oscillah; Millan, John; Carmone, Andrea; Dini, Mary; Palou, Theresa; Topma, Kum; Lavu, Evelyn; Markby, Jessica

    2014-01-01

    Laboratory-based CD4 monitoring of HIV patients presents challenges in resource limited settings (RLS) including frequent machine breakdown, poor engineering support and limited cold chain and specimen transport logistics. This study assessed the performance of two CD4 tests designed for use in RLS; the Dynal assay and the Alere PIMA test (PIMA). Accuracy of Dynal and PIMA using venous blood was assessed in a centralised laboratory by comparison to BD FACSCount (BD FACS). Dynal had a mean bias of -50.35 cells/µl (r(2) = 0.973, p<0.0001, n = 101) and PIMA -22.43 cells/µl (r(2)= 0.964, p<0.0001, n = 139) compared to BD FACS. Similar results were observed for PIMA operated by clinicians in one urban (n = 117) and two rural clinics (n = 98). Using internal control beads, PIMA precision was 10.34% CV (low bead mean 214.24 cells/µl) and 8.29% (high bead mean 920.73 cells/µl) and similar %CV results were observed external quality assurance (EQA) and replicate patient samples. Dynal did not perform using EQA and no internal controls are supplied by the manufacturer, however duplicate testing of samples resulted in r(2) = 0.961, p<0.0001, mean bias =  -1.44 cells/µl. Using the cut-off of 350 cells/µl compared to BD FACS, PIMA had a sensitivity of 88.85% and specificity of 98.71% and Dynal 88.61% and 100%. A total of 0.44% (2/452) of patient samples were misclassified as "no treat" and 7.30% (33/452) "treat" using PIMA whereas with Dynal 8.91% (9/101) as "treat" and 0% as "no treat". In our setting PIMA was found to be accurate, precise and user-friendly in both laboratory and clinic settings. Dynal performed well in initial centralized laboratory evaluation, however lacks requisite quality control measures, and was technically more difficult to use, making it less suitable for use at lower tiered laboratories.

  1. Sequence Classification: 893608 [

    Lifescience Database Archive (English)

    Full Text Available Sec9p, that binds v-SNARE Snc2p; also required for sporulation; syntaxin homolog that is functionally redundant with Sso2p; Sso1p || http://www.ncbi.nlm.nih.gov/protein/6325024 ...

  2. Visualizadores de imagens médicas gratuitos: é possível trabalhar apenas com eles? Freeware medical image viewers: can we rely only on them?

    Directory of Open Access Journals (Sweden)

    Filipe Ramos Barra

    2010-10-01

    Full Text Available OBJETIVO: Pesquisar visualizadores de imagens médicas gratuitos disponíveis na internet capazes de funcionar como cliente PACS (picture archiving and communication system e avaliar suas principais funções e a viabilidade do uso em computadores pessoais. MATERIAIS E MÉTODOS: Foi feita pesquisa, no Google e em sites especializados, por programas gratuitos disponíveis para o Windows. Foram encontrados cerca de 70, sendo 11 capazes de funcionar como cliente PACS, e selecionados seis destes para análise: ClearCanvas Workstation, KPACS, Onis, Synedra View Personal, Mito e Tudor DicomViewer. Com base nas necessidades dos autores, 16 funções foram avaliadas. RESULTADOS: Dos seis programas avaliados, dois possuem 10 das 16 funções avaliadas e um possui apenas duas. Três realizam MPR (reconstrução multiplanar, um realiza MIP (reconstrução por projeção de intensidade máxima, dois realizam VR (renderizações volumétricas, dois funcionam como servidor PACS, dois geram CDs, um realiza fusão de imagens, três permitem utilizar múltiplos monitores e apenas um não é compatível com Windows 7. CONCLUSÃO: Diversos programas gratuitos estão disponíveis e não existe nenhum completo. Cabe ao usuário analisar e selecionar o programa que melhor se enquadra nas suas necessidades, porém, os programas Onis, Synedra e ClearCanvas se destacam, cada um com suas peculiaridades. É totalmente viável o uso de programas gratuitos para o dia-a-dia do radiologista.OBJECTIVE: To search in the internet for freeware medical image viewers capable of running as a PACS (picture archiving and communication system client, and to evaluate their main functions as well as the feasibility of their use in personal computers. MATERIALS AND METHODS: The Google search engine and specialized sites were utilized in the search for freeware softwares for Windows. The authors have found about 70 and among them 11 were able to run as PACS clients. Six were selected for analysis: ClearCanvas Workstation, KPACS, Onis, Synedra View Personal, Mito and Tudor DicomViewer. Sixteen functions selected according to the authors' needs were evaluated. RESULTS: Among the six applications, two presented 10 of the 16 functions, and one of them presented only two. Three perform MPR (multiplanar reconstruction, one performs MIP (maximum intensity projection, two perform VR (volume rendering, two can run as a PACS server, two can create CDs, one performs images fusion, three allow the use of multiple monitors and only one is not compatible with Windows 7. CONCLUSION: Although several freeware applications are available, no one of them is complete. It is up to the users to analyze and select the software that best suits their needs. However, Onis, Synedra and ClearCanvas stand out because of their own peculiarities. The use of freeware image viewers is entirely feasible in the radiologists' daily routine.

  3. Short-term retention of a single word relies on retrieval from long-term memory when both rehearsal and refreshing are disrupted

    National Research Council Canada - National Science Library

    Rose, Nathan S; Buchsbaum, Bradley R; Craik, Fergus I. M

    2014-01-01

    Many working memory (WM) models propose that the focus of attention (or primary memory) has a capacity limit of one to four items, and therefore, that performance on WM tasks involves retrieving some items from long-term...

  4. Ways to Solving the problem of preferring Petition to relying on Law%破解"信访不信法"路在何方

    Institute of Scientific and Technical Information of China (English)

    陈青松

    2011-01-01

    "Petition" is normal and legitimate behavior for citizens to safeguard their rights. It is not the fundamental path to satisfy citizen demands, but in current system, is still an important defence line for social justice. Preferring petition to law is mainly a result that officials do not act or act improperly and government officials should bear primary responsibility, not the citizens. Many deficiencies in judicial process are important reasons for citizen to prefer petition rather than to laws. To alleviate the problem, the most fundamental path is to reinforce governance on laws including that CPC should hold reins of governance, government administration should be conducted according to the law, and judiciary administration should be impartial.%信访是民众维护自身权利的正常合法行为,虽然它不是解决民众诉求的根本路径,但在当前的体制下,仍是维护社会正义的重要防线.至于"信访不信法"现象的产生主要是官员不作为或乱作为的结果,应该是政府官员而不是民众对它负主要责任;司法过程本身存在的诸多不足也是民众信"访"超过信"法"的重要原因.当前要缓解"信访不信法"现象最根本的出路在于党依法执政、政府依法行政,司法机关公正司法,共同建设法治国家.

  5. Learners with Low Self-Efficacy for Information Literacy Rely on Library Resources Less Often But Are More Willing to Learn How to Use Them

    Directory of Open Access Journals (Sweden)

    Dominique Daniel

    2014-09-01

    Full Text Available Objectives – To determine whether there is a relationship between self-efficacy (i.e., confidence regarding information literacy skills and self-efficacy for distance learning; and to compare the use of electronic resources by high and low information literacy self-efficacy distance learners and their interest in learning more about searching. Design – Online survey. Setting – A small public university in the United States of America. Subjects – Undergraduate and graduate students enrolled in one or more online courses. Most respondents were in their twenties, 76% were female, 59% were undergraduates, and 69% were full time students. Methods – Students were asked six demographic questions, eight questions measuring their self-efficacy for information literacy, and four questions measuring their self-efficacy for online learning. All self-efficacy questions were adapted from previous studies and used a one to five Likert scale. The response rate was 6.2%. Correlational analysis was conducted to test the first two hypotheses (students who have higher self-efficacy for information seeking are more likely to have higher self-efficacy for online learning and for information manipulation. Descriptive analysis was used for the remaining hypotheses, to test whether students who have higher information literacy self-efficacy are more likely to have high library skills (hypothesis three and are more interested in learning about how to use library resources (hypothesis four. Among respondents high information literacy self-efficacy and low self-efficacy groups were distinguished, using the mean score of information literacy self-efficacy. Main Results – There was a significant correlation between self-efficacy for information seeking and self-efficacy for online learning (r = .27, as well as self-efficacy for information manipulation (r = .79. Students with high information seeking self-efficacy were more likely to use library databases (28.72%, while low self-efficacy respondents more often chose commercial search engines (30.98%. However those respondents were more likely to be interested in learning how to use library resources. Conclusion – Distance students with higher self-efficacy for information seeking and use also had higher self-efficacy for online learning. It is important to encourage such self-efficacy since studies have shown that it relates to better information literacy skills and a higher ability to be self-regulated learners. Confident learners process information, make effective decisions, and improve their learning more easily. Furthermore many respondents in this survey had little or false knowledge of how to use appropriate resources for their learning needs. This points to the need for effective library instruction. This study also shows that low self-efficacy students would like to have library instruction, especially to help them plan specific research assignments.

  6. A functional network of the tumor suppressors APC, hDlg, and PTEN, that relies on recognition of specific PDZ-domains.

    Science.gov (United States)

    Sotelo, Natalia S; Valiente, Miguel; Gil, Anabel; Pulido, Rafael

    2012-08-01

    APC and PTEN are tumor suppressor proteins that bind through their C-termini to the PDZ domain containing-hDlg scaffolding protein. We have found that co-expression of PTEN and hDlg enhanced the negative regulation of the PI3K/Akt pathway by PTEN, indicating the physiologic importance of these interactions. APC and PTEN share other PDZ domain containing-interacting partners, including the MAGI scaffolding proteins and the MAST family of protein kinases. Mutational analysis revealed that the C-terminal PDZ-binding motifs from APC and PTEN were differentially recognized by distinct PDZ domains. APC bound to the three PDZ domains from hDlg, whereas PTEN mainly bound to PDZ-2/hDlg. This indicates the existence of overlapping, but distinct PDZ-domain recognition patterns by APC and PTEN. Furthermore, a ternary complex formed by APC, PTEN, and hDlg was detected, suggesting that hDlg may serve as a platform to bring in proximity APC and PTEN tumor suppressor activities. In line with this, tumor-related mutations targeting the PDZ-2/hDlg domain diminished its interaction with APC and PTEN. Our results expand the PDZ-domain counterparts for the tumor suppressor APC, show that APC and PTEN share PDZ-domain partners but have individual molecular determinants for specific recognition of PDZ domains, and suggest the participation of the tumor suppressors APC, PTEN, and hDlg in PDZ-domain interaction networks which may be relevant in oncogenesis.

  7. Vardenafil demonstrates first-dose success and reliability of penetration and maintenance of erection in men with erectile dysfunction - RELY-II.

    Science.gov (United States)

    Valiquette, Luc; Montorsi, Francesco; Auerbach, Stephen

    2008-06-01

    Vardenafil has been shown to be efficacious in patients with erectile dysfunction (ED). We evaluated first-dose and repeat-dose response to vardenafil 20 mg. This randomized, placebo-controlled study consisted of a 4-week, treatment-free run-in phase; a 1-week, open-label challenge phase; and a 12-week, double-blind treatment phase. Primary efficacy was assessed in terms of reliability of insertion based on dose as measured by the Sexual Encounter Profile question 2 (SEP2). We assessed safety by evaluating adverse events (AEs). Baseline patient characteristics in the 2 treatment groups were similar. The most common comorbidities were hypertension (41%), dyslipidemia (28%) and diabetes mellitus (24%). Of the 573 patients receiving the 20-mg vardenafil challenge dose, 464 (81%) achieved first-time successful penetration (SEP2), and 401 (70%) reported successful erection maintenance (SEP3). Patients receiving vardenafil 20 mg had statistically (p reliability of insertion was seen within the first 4 weeks of treatment. Vardenafil therapy was statistically (p < 0.001) and clinically superior to placebo for all secondary efficacy end points as well. Most AEs associated with vardenafil were mild to moderate, with headache, flushing and nasal congestion most frequently reported. Vardenafil 20 mg had a high first-dose success rate for both SEP2 (81%) and SEP3 (70%); this was maintained through to the study end point (85% for SEP2 and 78% for SEP3). These findings were achieved in men with frequently associated comorbidities including hypertension, dyslipidemia and diabetes.

  8. Enhancing drought relief comprehensive capacity relying on plan%依托规划全面提升抗旱减灾综合能力

    Institute of Scientific and Technical Information of China (English)

    万群志

    2013-01-01

    简要介绍了《全国抗旱规划》编制背景和基本情况,分析了抗旱应急水源工程、旱情监测预警系统、抗旱服务组织等抗旱规划主要建设内容的建设管理现状和发挥的抗旱作用,探讨了抗旱规划组织实施工作中存在的主要问题和困难,并有针对性地提出了加快推进《全国抗旱规划》实施的政策建议和相关措施.

  9. Relying on the library constructing joint knowledge aid system assumption%依托图书馆构建联合知识援助体系设想

    Institute of Scientific and Technical Information of China (English)

    唐高芳

    2012-01-01

    论述了知识援助的内含及重要意义,分析了图书馆在知识援助中的重要作用,结合国内外知识援助的一些做法,提出了依托图书馆构建联合知识援助体系的设想。%This paper discusses the contents and the important significance of knowledge aid, analyzing on the important role of the library in knowledge aid, combining with the aid of some domestic and foreign knowledge based on practice, and puts forward the library construction knowledge aid system of the joint assumption.

  10. Apicoplast-Localized Lysophosphatidic Acid Precursor Assembly Is Required for Bulk Phospholipid Synthesis in Toxoplasma gondii and Relies on an Algal/Plant-Like Glycerol 3-Phosphate Acyltransferase.

    Directory of Open Access Journals (Sweden)

    Souad Amiar

    2016-08-01

    Full Text Available Most apicomplexan parasites possess a non-photosynthetic plastid (the apicoplast, which harbors enzymes for a number of metabolic pathways, including a prokaryotic type II fatty acid synthesis (FASII pathway. In Toxoplasma gondii, the causative agent of toxoplasmosis, the FASII pathway is essential for parasite growth and infectivity. However, little is known about the fate of fatty acids synthesized by FASII. In this study, we have investigated the function of a plant-like glycerol 3-phosphate acyltransferase (TgATS1 that localizes to the T. gondii apicoplast. Knock-down of TgATS1 resulted in significantly reduced incorporation of FASII-synthesized fatty acids into phosphatidic acid and downstream phospholipids and a severe defect in intracellular parasite replication and survival. Lipidomic analysis demonstrated that lipid precursors are made in, and exported from, the apicoplast for de novo biosynthesis of bulk phospholipids. This study reveals that the apicoplast-located FASII and ATS1, which are primarily used to generate plastid galactolipids in plants and algae, instead generate bulk phospholipids for membrane biogenesis in T. gondii.

  11. Growth of triple-negative breast cancer cells relies upon coordinate autocrine expression of the proinflammatory cytokines IL-6 and IL-8.

    Science.gov (United States)

    Hartman, Zachary C; Poage, Graham M; den Hollander, Petra; Tsimelzon, Anna; Hill, Jamal; Panupinthu, Nattapon; Zhang, Yun; Mazumdar, Abhijit; Hilsenbeck, Susan G; Mills, Gordon B; Brown, Powel H

    2013-06-01

    Triple-negative breast cancers (TNBC) are aggressive with no effective targeted therapies. A combined database analysis identified 32 inflammation-related genes differentially expressed in TNBCs and 10 proved critical for anchorage-independent growth. In TNBC cells, an LPA-LPAR2-EZH2 NF-κB signaling cascade was essential for expression of interleukin (IL)-6, IL-8, and CXCL1. Concurrent inhibition of IL-6 and IL-8 expression dramatically inhibited colony formation and cell survival in vitro and stanched tumor engraftment and growth in vivo. A Cox multivariable analysis of patient specimens revealed that IL-6 and IL-8 expression predicted patient survival times. Together these findings offer a rationale for dual inhibition of IL-6/IL-8 signaling as a therapeutic strategy to improve outcomes for patients with TNBCs.

  12. 以综合大学为依托培养各类优秀体育人才%Cultivating various excellent sports talents by relying on multiversities

    Institute of Scientific and Technical Information of China (English)

    胡红梅; 彭洪涛

    2007-01-01

    从社会发展对新型体育人才的需求出发,对综合性大学发展体育新兴学科的优势和可行性进行分析.认为社会发展对体育人才的需求在类别上和层次上都提出了更高的要求,现在的体育人才培养模式已不能满足社会的需求.综合性大学建设和发展体育新兴学科具有人文、学术环境优势,多学科优势.综合性大学发展体育学科符合国家体育科技发展战略,资源共享能解决学科建设与资源不足的矛盾,学科渗透为体育学科提供了发展空间,具有切实可行性.并且探讨了在综合性大学发展体育新兴学科的具体措施.

  13. Relying on the National Mobile Disaster Hospital as a business continuity strategy in the aftermath of a tornado: The Louisville experience.

    Science.gov (United States)

    Kearns, Randy D; Stringer, Lew; Craig, James; Godette-Crawford, Regina; Black, Paul S; Andra, David L; Winslow, James

    2017-01-01

    On 28th April, 2014, a tornado left much of Louisville, MS and the local hospital, Winston Medical Center, in ruin. In the USA, temporary hospital solutions have been used to augment the mainstream healthcare system since the American Civil War. As memories fade, however, the necessary readiness for a temporary hospital also fades, at times leaving a patchwork of resources either underfunded or abandoned. With the creation of the Hospital Preparedness Program, several temporary hospital solutions were created in various states across the USA. In the present case, Mississippi and North Carolina resources were used in Louisville in the aftermath of a direct impact that destroyed the hospital and nursing home. In the hours that followed, after lives were saved and patients safely relocated, a frank assessment confirmed the structural loss of the hospital. Local emergency responders, hospital staff, state and federal representatives all rallied with the aim of saving the community's only hospital. The steps taken in Louisville and the deliberate restoration of these essential services offer a learning opportunity for all involved in healthcare disaster preparedness, response and recovery.

  14. Adiponectin regulates bone mass accrual through two opposite mechanisms, one local and one central, that both rely on FoxO1

    Science.gov (United States)

    Kajimura, Daisuke; Lee, Ha Won; Riley, Kyle J.; Arteaga-Solis, Emilio; Ferron, Mathieu; Zhou, Bin; Clarke, Christopher J.; Hannun, Yusuf A.; DePinho, Ronald A; Guo, Edward X.; Mann, J. John; Karsenty, Gerard

    2013-01-01

    Summary The synthesis of adiponectin, an adipokine with ill-defined functions in animals fed a normal diet, is enhanced by the osteoblast-derived hormone osteocalcin. Here we show that adiponectin signals back in osteoblasts to hamper their proliferation and favor their apoptosis, altogether decreasing bone mass and circulating osteocalcin levels. Adiponectin fulfills these functions, independently of its known receptors and signaling pathways, by decreasing FoxO1 activity in a PI3 kinase-dependent manner. Overtime however, these local effects are masked because adiponectin signals in neurons of the locus coeruleus, also through FoxO1, to decrease the sympathetic tone thereby increasing bone mass, and decreasing energy expenditure. This study reveals that adiponectin has the unusual ability to regulate the same function in two opposite manners depending on where it acts and that it opposes, partially, leptin’s influence on the sympathetic nervous system. It also proposes that adiponectin regulation of bone mass occurs through a PI3 kinase-FoxO1 pathway. PMID:23684624

  15. Apoptotic DNA Degradation into Oligonucleosomal Fragments, but Not Apoptotic Nuclear Morphology, Relies on a Cytosolic Pool of DFF40/CAD Endonuclease*

    Science.gov (United States)

    Iglesias-Guimarais, Victoria; Gil-Guiñon, Estel; Gabernet, Gisela; García-Belinchón, Mercè; Sánchez-Osuna, María; Casanelles, Elisenda; Comella, Joan X.; Yuste, Victor J.

    2012-01-01

    Apoptotic cell death is characterized by nuclear fragmentation and oligonucleosomal DNA degradation, mediated by the caspase-dependent specific activation of DFF40/CAD endonuclease. Here, we describe how, upon apoptotic stimuli, SK-N-AS human neuroblastoma-derived cells show apoptotic nuclear morphology without displaying concomitant internucleosomal DNA fragmentation. Cytotoxicity afforded after staurosporine treatment is comparable with that obtained in SH-SY5Y cells, which exhibit a complete apoptotic phenotype. SK-N-AS cell death is a caspase-dependent process that can be impaired by the pan-caspase inhibitor q-VD-OPh. The endogenous inhibitor of DFF40/CAD, ICAD, is correctly processed, and dff40/cad cDNA sequence does not reveal mutations altering its amino acid composition. Biochemical approaches show that both SH-SY5Y and SK-N-AS resting cells express comparable levels of DFF40/CAD. However, the endonuclease is poorly expressed in the cytosolic fraction of healthy SK-N-AS cells. Despite this differential subcellular distribution of DFF40/CAD, we find no differences in the subcellular localization of both pro-caspase-3 and ICAD between the analyzed cell lines. After staurosporine treatment, the preferential processing of ICAD in the cytosolic fraction allows the translocation of DFF40/CAD from this fraction to a chromatin-enriched one. Therefore, the low levels of cytosolic DFF40/CAD detected in SK-N-AS cells determine the absence of DNA laddering after staurosporine treatment. In these cells DFF40/CAD cytosolic levels can be restored by the overexpression of their own endonuclease, which is sufficient to make them proficient at degrading their chromatin into oligonucleosome-size fragments after staurosporine treatment. Altogether, the cytosolic levels of DFF40/CAD are determinants in achieving a complete apoptotic phenotype, including oligonucleosomal DNA degradation. PMID:22253444

  16. System-Events Toolbox--Activating Urban Places for Social Cohesion through Designing a System of Events That Relies on Local Resources

    Science.gov (United States)

    Fassi, Davide; Motter, Roberta

    2014-01-01

    This paper is a reflection on the use of public spaces in towns and the development of a system-events toolbox to activate them towards social cohesion. It is the result of a 1 year action research developed together with POLIMI DESIS Lab of the Department of Design to develop design solutions to open up the public spaces of the campus to the…

  17. No transfer between conditions in balance training regimes relying on tasks with different postural demands: Specificity effects of two different serious games.

    Science.gov (United States)

    Naumann, Tim; Kindermann, Stefan; Joch, Michael; Munzert, Jörn; Reiser, Mathias

    2015-03-01

    Despite the increasing use of video games involving whole body movements to enhance postural control in health prevention and rehabilitation, there is no consistent proof that training effects actually transfer to other balance tasks. The present study aimed to determine whether training effects on two different video-game-based training devices were task-specific or could be transferred to either postural control in quiet stance or to performance on the other device. 37 young healthy adults were split into three groups: two intervention groups that trained for 30min on either the Nintendo(®) Wii Fit Balance Board or the MFT Challenge Disc(®) three times per week for 4 weeks and a control group that received no training. All games require participants to control virtual avatars by shifting the center of mass in different directions. Both devices differ in their physical properties. The Balance Board provides a stable surface, whereas the Challenge Disc can be tilted in all directions. Dependent variables were the game scores on both devices and the center of pressure (COP) displacements measured via force plate. At posttest, both intervention groups showed significant increases in performance on the trained games compared to controls. However, there were no relevant transfer effects to performance on the untrained device and no changes in COP path length in quiet stance. These results suggest that training effects on both devices are highly specific and do not transfer to tasks with different postural demands.

  18. Cryotolerance of porcine in vitro-produced blastocysts relies on blastocyst stage and length of in vitro culture prior to vitrification.

    Science.gov (United States)

    Morató, Roser; Castillo-Martín, Míriam; Yeste, Marc; Bonet, Sergi

    2014-12-04

    The aim of our study was to assess whether the cryotolerance of in vitro-produced embryos could be influenced by the length of in vitro culture and size of blastocoel cavity before vitrification, using the pig as a model. For this purpose we analysed the cryoresistance and apoptosis rate of blastocysts at different stages of development as derived on Day 5 and 6 of in vitro culture. Blastocysts were subsequently vitrified, warmed and cultured for 24h. Re-expansion rates were recorded at 3 and 24h and total cell number and apoptotic cells were determined at 24h. Day-6 blastocysts showed the highest rates of survival after warming, which indicates higher quality compared with Day-5 blastocysts. Higher re-expansion rates were observed for expanded blastocysts and those in the process of hatching when compared with early blastocysts. Total cell number and apoptotic cells were affected by blastocyst stage, vitrification-warming procedures and length of in vitro culture, as expanding and hatching-hatched blastocysts from Day 6 presented higher percentages of apoptotic cells than fresh blastocysts and blastocysts vitrified at Day 5. Our findings suggest that the cryotop vitrification method is useful for the cryopreservation of porcine blastocysts presenting a high degree of expansion, particularly when vitrification is performed after 6 days of in vitro culture. Furthermore, these results show that faster embryo development underlies higher blastocyst cryotolerance and provide evidence that blastocoel cavity expansion before vitrification is a reliable index of in vitro-produced embryo quality and developmental potential.

  19. Mechanisms of NMDA Receptor- and Voltage-Gated L-Type Calcium Channel-Dependent Hippocampal LTP Critically Rely on Proteolysis That Is Mediated by Distinct Metalloproteinases.

    Science.gov (United States)

    Wiera, Grzegorz; Nowak, Daria; van Hove, Inge; Dziegiel, Piotr; Moons, Lieve; Mozrzymas, Jerzy W

    2017-02-01

    Long-term potentiation (LTP) is widely perceived as a memory substrate and in the hippocampal CA3-CA1 pathway, distinct forms of LTP depend on NMDA receptors (nmdaLTP) or L-type voltage-gated calcium channels (vdccLTP). LTP is also known to be effectively regulated by extracellular proteolysis that is mediated by various enzymes. Herein, we investigated whether in mice hippocampal slices these distinct forms of LTP are specifically regulated by different metalloproteinases (MMPs). We found that MMP-3 inhibition or knock-out impaired late-phase LTP in the CA3-CA1 pathway. Interestingly, late-phase LTP was also decreased by MMP-9 blockade. When both MMP-3 and MMP-9 were inhibited, both early- and late-phase LTP was impaired. Using immunoblotting, in situ zymography, and immunofluorescence, we found that LTP induction was associated with an increase in MMP-3 expression and activity in CA1 stratum radiatum. MMP-3 inhibition and knock-out prevented the induction of vdccLTP, with no effect on nmdaLTP. L-type channel-dependent LTP is known to be impaired by hyaluronic acid digestion. We found that slice treatment with hyaluronidase occluded the effect of MMP-3 blockade on LTP, further confirming a critical role for MMP-3 in this form of LTP. In contrast to the CA3-CA1 pathway, LTP in the mossy fiber-CA3 projection did not depend on MMP-3, indicating the pathway specificity of the actions of MMPs. Overall, our study indicates that the activation of perisynaptic MMP-3 supports L-type channel-dependent LTP in the CA1 region, whereas nmdaLTP depends solely on MMP-9. Various types of long-term potentiation (LTP) are correlated with distinct phases of memory formation and retrieval, but the underlying molecular signaling pathways remain poorly understood. Extracellular proteases have emerged as key players in neuroplasticity phenomena. The present study found that L-type calcium channel-dependent LTP in the CA3-CA1 hippocampal projection is critically regulated by the activity of matrix metalloprotease 3 (MMP-3), in contrast to NMDAR-dependent LTP regulated by MMP-9. Moreover, the induction of LTP was associated with an increase in MMP-3 expression and activity. Finally, we found that the digestion of hyaluronan, a principal extracellular matrix component, disrupted the MMP-3-dependent component of LTP. These results indicate that distinct MMPs might act as molecular switches for specific types of LTP. Copyright © 2017 the authors 0270-6474/17/371240-17$15.00/0.

  20. ERPs show that classroom-instructed late second language learners rely on the same prosodic cues in syntactic parsing as native speakers.

    Science.gov (United States)

    Nickels, Stefanie; Opitz, Bertram; Steinhauer, Karsten

    2013-12-17

    The loss of brain plasticity after a 'critical period' in childhood has often been argued to prevent late language learners from using the same neurocognitive mechanisms as native speakers and, therefore, from attaining a high level of second language (L2) proficiency [7,11]. However, more recent behavioral and electrophysiological research has challenged this 'Critical Period Hypothesis', demonstrating that even late L2 learners can display native-like performance and brain activation patterns [17], especially after longer periods of immersion in an L2 environment. Here we use event-related potentials (ERPs) to show that native-like processing can also be observed in the largely under-researched domain of speech prosody - even when L2 learners are exposed to their second language almost exclusively in a classroom setting. Participants listened to spoken sentences whose prosodic boundaries would either cooperate or conflict with the syntactic structure. Previous work had shown that this paradigm is difficult for elderly native speakers, however, German L2 learners of English showed very similar ERP components for on-line prosodic phrasing as well as for prosody-syntax mismatches (garden path effects) as the control group of native speakers. These data suggest that L2 immersion is not always necessary to master complex L2 speech processing in a native-like way. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  1. Adenosine Diphosphate Ribosylation Factor-GTPaseActivating Protein Stimulates the Transport of AUX1Endosome, Which Relies on Actin Cytoskeletal Organization in Rice Root DevelopmentF

    Institute of Scientific and Technical Information of China (English)

    Cheng Du; Yunyuan XU; Yingdian Wang; Kang Chong

    2011-01-01

    Polar auxin transport,which depends on polarized subcellular distribution of AUXIN RESISTANT 1/LIKE AUX1 (AUX1/LAX) influx carriers and PIN-FORMED (PIN) efflux carriers,mediates various processes of plant growth and development.Endosomal recycling of PIN1 is mediated by an adenosine diphosphate (ADP)ribosylation factor (ARF)-GTPase exchange factor protein,GNOM.However,the mediation of auxin influx carrier recycling is poorly understood.Here,we report that overexpression of OsAGAP,an ARF-GTPase-activating protein in rice,stimulates vesicle transport from the plasma membrane to the Golgi apparatus in protoplasts and transgenic plants and induces the accumulation of early endosomes and AUX1.AUX1 endosomes could partially colocalize with FM4-64 labeled early endosome after actin disruption.Furthermore,OsAGAP is involved in actin cytoskeletal organization,and its overexpression tends to reduce the thickness and bundling of actin filaments.Fluorescence recovery after photobleaching analysis revealed exocytosis of the AUX1 recycling endosome was not affected in the OsAGAP overexpression cells,and was only slightly promoted when the actin filaments were completely disrupted by Lat B.Thus,we propose that AUX1 accumulation in the OsAGAP overexpression and actin disrupted cells may be due to the fact that endocytosis of the auxin influx carrier AUX1 early endosome was greatly promoted by actin cytoskeleton disruption.

  2. Fine Needle Aspiration and Medullary Thyroid Carcinoma: The Risk of Inadequate Preoperative Evaluation and Initial Surgery When Relying Upon FNAB Cytology Alone

    NARCIS (Netherlands)

    Essig, G.F.; Porter, K.; Schneider, D.; Debora, A.; Lindsey, S.C.; Busonero, G.; Fineberg, D.; Fruci, B.; Boelaert, K.; Smit, J.W.A.; Meijer, J.A.M.; Duntas, L.; Sharma, N.; Costante, G.; Filetti, S.; Sippel, R.S.; Biondi, B.; Topliss, D.J.; Pacini, F.; Maciel, R.M.; Walz, P.C.; Kloos, R.T.

    2013-01-01

    Objectives: To evaluate the diagnostic accuracy of fine-needle aspiration biopsy (FNAB) to preoperatively diagnose medullary thyroid cancer (MTC) among multiple international centers and evaluate how the cytological diagnosis alone could impact patient management.Methods: We performed a retrospectiv

  3. Anti-Aspergillus human host defence relies on type 1 T helper (Th1), rather than type 17 T helper (Th17), cellular immunity

    NARCIS (Netherlands)

    L.Y.A. Chai (Louis); F. van de Veerdonk (Frank); R.J. Marijnissen (Renoud); S.C. Cheng; A.L. Khoo; M. Hectors (Magda); K. Lagrou (Katrien); A.G. Vonk (Alieke); J. Maertens (Johan); L.A.B. Joosten (Leo); B.J. Kullberg (Bart Jan); M.G. Netea (Mihai)

    2010-01-01

    textabstractSummary Both interferon-γ-producing type 1 T helper (Th1)- and interleukin-17 (IL-17)-producing Th17 cells have been proposed to be involved in anti-fungal host defence. Although invasive aspergillosis is one of the most severe human fungal infections, little is known regarding the relat

  4. Anti-Aspergillus human host defence relies on type 1 T helper (Th1), rather than type 17 T helper (Th17), cellular immunity.

    NARCIS (Netherlands)

    Chai, L.; Veerdonk, F.L. van de; Marijnissen, R.J.; Cheng, S.C.; Khoo, A.L.; Hectors, M.P.C.; Lagrou, K.; Vonk, A.G.; Maertens, J.; Joosten, L.A.B.; Kullberg, B.J.; Netea, M.G.

    2010-01-01

    Both interferon-gamma-producing type 1 T helper (Th1)- and interleukin-17 (IL-17)-producing Th17 cells have been proposed to be involved in anti-fungal host defence. Although invasive aspergillosis is one of the most severe human fungal infections, little is known regarding the relative importance o

  5. Protection against Paracoccidioides brasiliensis infection in mice treated with modulated dendritic cells relies on inhibition of interleukin-10 production by CD8+ T cells

    Science.gov (United States)

    Alves da Costa, Thiago; Di Gangi, Rosária; Martins, Paula; Longhini, Ana Leda Figueiredo; Zanucoli, Fábio; de Oliveira, Alexandre Leite Rodrigues; Stach-Machado, Dagmar Ruth; Burger, Eva; Verinaud, Liana; Thomé, Rodolfo

    2015-01-01

    Paracoccidioidomycosis is a systemic infection prevalent in Latin American countries. Disease develops after inhalation of Paracoccidioides brasiliensis conidia followed by an improper immune activation by the host leucocytes. Dendritic cells (DCs) are antigen-presenting cells with the unique ability to direct the adaptive immune response by the time of activation of naive T cells. This study was conducted to test whether extracts of P. brasiliensis would induce maturation of DCs. We found that DCs treated with extracts acquired an inflammatory phenotype and upon adoptive transfer conferred protection to infection. Interestingly, interleukin-10 production by CD8+ T cells was ablated following DC transfer. Further analyses showed that lymphocytes from infected mice were high producers of interleukin-10, with CD8+ T cells being the main source. Blockage of cross-presentation to CD8+ T cells by modulated DCs abolished the protective effect of adoptive transfer. Collectively, our data show that adoptive transfer of P. brasiliensis-modulated DCs is an interesting approach for the control of infection in paracoccidioidomycosis. PMID:26302057

  6. Prolonged propagation of rat neural stem cells relies on inhibiting autocrine/paracrine bone morphogenetic protein and platelet derived growth factor signals

    Institute of Scientific and Technical Information of China (English)

    Yirui Sun; Liangfu Zhou; Xing Wu; Hua Liu; Qiang Yuan; Ying Mao; Jin Hu

    2011-01-01

    Continuous expansion of rat neural stem cell lines has not been achieved due to proliferation arrest and spontaneous differentiation in vitro. In the current study, neural precursor cells derived from the subventricular zone of adult rats spontaneously underwent astroglial and oligodendroglial differentiation after limited propagation. This differentiation was largely induced by autocrine or paracrine bone morphogenetic protein and platelet derived growth factor signals. The results showed that, by inhibiting bone morphogenetic protein and platelet derived growth factor signals, adult rat neural precursor cells could be extensively cultured in vitro as tripotent stem cell lines. In addition to adult rat neural stem cells, we found that bone morphogenetic protein antagonists can promote the proliferation of human neural stem cells. Therefore, the present findings illustrated the role of autocrine or paracrine bone morphogenetic protein and platelet derived growth factor signaling in determining neural stem cell self-renewal and differentiation. By antagonizing both signals, the long-term propagation of rat neural stem cell lines can be achieved.

  7. Chip-interleaved optical code division multiple access relying on a photon-counting iterative successive interference canceller for free-space optical channels.

    Science.gov (United States)

    Zhou, Xiaolin; Zheng, Xiaowei; Zhang, Rong; Hanzo, Lajos

    2013-07-01

    In this paper, we design a novel Poisson photon-counting based iterative successive interference cancellation (SIC) scheme for transmission over free-space optical (FSO) channels in the presence of both multiple access interference (MAI) as well as Gamma-Gamma atmospheric turbulence fading, shot-noise and background light. Our simulation results demonstrate that the proposed scheme exhibits a strong MAI suppression capability. Importantly, an order of magnitude of BER improvements may be achieved compared to the conventional chip-level optical code-division multiple-access (OCDMA) photon-counting detector.

  8. A photon-counting spatial-diversity-and-multiplexing MIMO scheme for Poisson atmospheric channels relying on Q-ary PPM.

    Science.gov (United States)

    Zhou, Xiaolin; Zhang, Dingchen; Zhang, Rong; Hanzo, Lajos

    2012-11-19

    A novel Photon-Counting Spatial-Diversity-and-Multiplexing (PC-SDM) scheme is proposed for high-speed Free-Space Optical (FSO) transmission over shot-noise limited Poisson channels experiencing turbulence-induced fading. In particular, Iterative Parallel Interference Cancellation (Iter-PIC) aided Q-ary Pulse Position Modulation (Q-PPM) is employed. Simulation results demonstrate that our proposed scheme exhibits a high integrity and a high throughput, while mitigating the effects of multi-stream interference and background radiation noise.

  9. Can we rely on simulated patients' satisfaction with their consultation for assessing medical students' communication skills? A cross-sectional study.

    Science.gov (United States)

    Gude, T; Grimstad, H; Holen, A; Anvik, T; Baerheim, A; Fasmer, O B; Hjortdahl, P; Vaglum, P

    2015-12-18

    In medical education, teaching methods offering intensive practice without high utilization of faculty resources are needed. We investigated whether simulated patients' (SPs') satisfaction with a consultation could predict professional observers' assessment of young doctors' communication skills. This was a comparative cross-sectional study of 62 videotaped consultations in a general practice setting with young doctors who were finishing their internship. The SPs played a female patient who had observed blood when using the toilet, which had prompted a fear of cancer. Immediately afterwards, the SP rated her level of satisfaction with the consultation, and the scores were dichotomized into satisfaction or dissatisfaction. Professional observers viewed the videotapes and assessed the doctors' communication skills using the Arizona Communication Interview Rating Scale (ACIR). Their ratings of communication skills were dichotomized into acceptable versus unacceptable levels of competence. The SPs' satisfaction showed a predictive power of 0.74 for the observers' assessment of the young doctors and whether they reached an acceptable level of communication skills. The SPs' dissatisfaction had a predictive power of 0.71 for the observers' assessment of an unacceptable communication level. The two assessment methods differed in 26% of the consultations. When SPs felt relief about their cancer concern after the consultation, they assessed the doctors' skills as satisfactory independent of the observers' assessment. Accordance between the dichotomized SPs' satisfaction score and communication skills assessed by observers (using the ACIR) was in the acceptable range. These findings suggest that SPs' satisfaction scores may provide a reliable source for assessing communication skills in educational programs for medical trainees (students and young doctors). Awareness of the patient's concerns seems to be of vital importance to patient satisfaction.

  10. Biconcave shape of human red-blood-cell ghosts relies on density differences between the rim and dimple of the ghost's plasma membrane.

    Science.gov (United States)

    Hoffman, Joseph F

    2016-12-20

    The shape of the human red blood cell is known to be a biconcave disk. It is evident from a variety of theoretical work that known physical properties of the membrane, such as its bending energy and elasticity, can explain the red-blood-cell biconcave shape as well as other shapes that red blood cells assume. But these analyses do not provide information on the underlying molecular causes. This paper describes experiments that attempt to identify some of the underlying determinates of cell shape. To this end, red-blood-cell ghosts were made by hypotonic hemolysis and then reconstituted such that they were smooth spheres in hypo-osmotic solutions and smooth biconcave discs in iso-osmotic solutions. The spherical ghosts were centrifuged onto a coated coverslip upon which they adhered. When the attached spheres were changed to biconcave discs by flushing with an iso-osmotic solution, the ghosts were observed to be mainly oriented in a flat alignment on the coverslip. This was interpreted to mean that, during centrifugation, the spherical ghosts were oriented by a dense band in its equatorial plane, parallel to the centrifugal field. This appears to be evidence that the difference in the densities between the rim and the dimple regions of red blood cells and their ghosts may be responsible for their biconcave shape.

  11. Rabies virus infection induces type I interferon production in an IPS-1 dependent manner while dendritic cell activation relies on IFNAR signaling.

    Science.gov (United States)

    Faul, Elizabeth J; Wanjalla, Celestine N; Suthar, Mehul S; Gale, Michael; Wirblich, Christoph; Schnell, Matthias J

    2010-07-22

    As with many viruses, rabies virus (RABV) infection induces type I interferon (IFN) production within the infected host cells. However, RABV has evolved mechanisms by which to inhibit IFN production in order to sustain infection. Here we show that RABV infection of dendritic cells (DC) induces potent type I IFN production and DC activation. Although DCs are infected by RABV, the viral replication is highly suppressed in DCs, rendering the infection non-productive. We exploited this finding in bone marrow derived DCs (BMDC) in order to differentiate which pattern recognition receptor(s) (PRR) is responsible for inducing type I IFN following infection with RABV. Our results indicate that BMDC activation and type I IFN production following a RABV infection is independent of TLR signaling. However, IPS-1 is essential for both BMDC activation and IFN production. Interestingly, we see that the BMDC activation is primarily due to signaling through the IFNAR and only marginally induced by the initial infection. To further identify the receptor recognizing RABV infection, we next analyzed BMDC from Mda-5-/- and RIG-I-/- mice. In the absence of either receptor, there is a significant decrease in BMDC activation at 12h post infection. However, only RIG-I-/- cells exhibit a delay in type I IFN production. In order to determine the role that IPS-1 plays in vivo, we infected mice with pathogenic RABV. We see that IPS-1-/- mice are more susceptible to infection than IPS-1+/+ mice and have a significantly increased incident of limb paralysis.

  12. Rabies virus infection induces type I interferon production in an IPS-1 dependent manner while dendritic cell activation relies on IFNAR signaling.

    Directory of Open Access Journals (Sweden)

    Elizabeth J Faul

    Full Text Available As with many viruses, rabies virus (RABV infection induces type I interferon (IFN production within the infected host cells. However, RABV has evolved mechanisms by which to inhibit IFN production in order to sustain infection. Here we show that RABV infection of dendritic cells (DC induces potent type I IFN production and DC activation. Although DCs are infected by RABV, the viral replication is highly suppressed in DCs, rendering the infection non-productive. We exploited this finding in bone marrow derived DCs (BMDC in order to differentiate which pattern recognition receptor(s (PRR is responsible for inducing type I IFN following infection with RABV. Our results indicate that BMDC activation and type I IFN production following a RABV infection is independent of TLR signaling. However, IPS-1 is essential for both BMDC activation and IFN production. Interestingly, we see that the BMDC activation is primarily due to signaling through the IFNAR and only marginally induced by the initial infection. To further identify the receptor recognizing RABV infection, we next analyzed BMDC from Mda-5-/- and RIG-I-/- mice. In the absence of either receptor, there is a significant decrease in BMDC activation at 12h post infection. However, only RIG-I-/- cells exhibit a delay in type I IFN production. In order to determine the role that IPS-1 plays in vivo, we infected mice with pathogenic RABV. We see that IPS-1-/- mice are more susceptible to infection than IPS-1+/+ mice and have a significantly increased incident of limb paralysis.

  13. At the Scene of the Crime: Law Enforcement Agencies and Lawmakers Rely on Studies from the Institute for Social Research To Inform Policy-Making.

    Science.gov (United States)

    Maurer, Rachel

    1998-01-01

    The University of New Mexico's Institute for Social Research operates eight centers that conduct policy-related research on criminal justice, criminology, delinquency, and social problems. Projects have focused on inequities in criminal sentencing in New Mexico, juveniles' access to and use of guns, New Mexico's need for a juvenile prison, and the…

  14. Do clinicians decide relying primarily on Bayesians principles or on Gestalt perception? Some pearls and pitfalls of Gestalt perception in medicine.

    Science.gov (United States)

    Cervellin, Gianfranco; Borghi, Loris; Lippi, Giuseppe

    2014-08-01

    Clinical judgment is a foundation of medical practice and lies at the heart of a physician's knowledge, expertise and skill. Although clinical judgment is an active part of all medical fields, thus including diagnosis and therapy, communication and decision making, it is still poorly defined. It can be considered a synthesis of intuition (mainly based on Gestalt principles) and an analytical approach. Gestalt perception finds its rationale in the evidence that perception of any given object or experience exhibits intrinsic qualities that cannot be completely reduced to visual, auditory, tactile, olfactory, or gustatory components. Thus, perceptions are not constructed in a "bottom-up" fashion from such elements, but are instead globally perceived, in a more "top-down" fashion. Gestalt perception, if cautiously and carefully combined with structured (techno)logical tools, would permit one to defoliate the often too-many-branches built diagnostic trees, and help physicians to better develop their competency. On the other hand, the practice of evidence-based medicine lies in the integration of individual clinical expertise and judgment with the best available external clinical evidence from systematic research. This article is aimed at providing some general concepts about Gestalt perception, and to discuss some aspects of clinical practice potentially influenced by this approach.

  15. Transcriptomic analysis by RNA-seq reveals AP-1 pathway as key regulator that green tea may rely on to inhibit lung tumorigenesis.

    Science.gov (United States)

    Pan, Jing; Zhang, Qi; Xiong, Donghai; Vedell, Peter; Yan, Ying; Jiang, Hui; Cui, Peng; Ding, Feng; Tichelaar, Jay W; Wang, Yian; Lubet, Ronald A; You, Ming

    2014-01-01

    Green tea is a promising chemopreventive agent for lung cancer. Multiple signaling events have been reported, however, the relative importance of these mechanisms in mediating the chemopreventive function of green tea is unclear. In the present study, to examine the involvement of AP-1 in green tea polyphenols induced tumor inhibition, human NSCLC cell line H1299 and mouse SPON 10 cells were identified as AP-1 dependent, as these two lines exhibit high constitutive AP-1 activity, and when TAM67 expression was induced with doxycycline, cell growth was inhibited and correlated with suppressed AP-1 activity. RNA-seq was used to determine the global transcriptional effects of AP-1 inhibition and also uncover the possible involvement of AP-1 in tea polyphenols induced chemoprevention. TAM67 mediated changes in gene expression were identified, and within down-regulated genes, AP-1 was identified as a key transcription regulator. RNA-seq analysis revealed that Polyphenon E-treated cells shared 293 commonly down-regulated genes within TAM67 expressing H1299 cells, and by analysis of limited Chip-seq data, over 10% of the down-regulated genes contain a direct AP-1 binding site, indicating that Polyphenon E elicits chemopreventive activity by regulating AP-1 target genes. Conditional TAM67 expressing transgenic mice and NSCLC cell lines were used to further confirm that the chemopreventive activity of green tea is AP-1 dependent. Polyphenon E lost its chempreventive function both in vitro and in vivo when AP-1 was inhibited, indicating that AP-1 inhibition is a major pathway through which green tea exhibits chemopreventive effects.

  16. N-methyl-D-aspartate receptor inhibition by an apolipoprotein E-derived peptide relies on low-density lipoprotein receptor-associated protein.

    Science.gov (United States)

    Sheng, Zhenyu; Prorok, Mary; Brown, Brigid E; Castellino, Francis J

    2008-08-01

    The effects of a synthetic apoE peptide, viz., residues 133-149 (apoE[133-149]), a mimetic that comprises the apoE receptor binding domain, on N-methyl-D-aspartate (NMDA)/glycine-induced ion flow through NMDA receptor (NMDAR) channels, have been investigated. The activity of apoE[133-149] was found to depend on the low-density lipoprotein receptor-related protein (LRP). Competition experiments with receptor-associated protein (RAP) and activated alpha(2)-macroglobulin (alpha(2)M*), two proteins that compete for apoE binding to LRP, demonstrate that apoE[133-149] inhibition of NMDAR function is mediated at a locus in LRP that overlaps with the binding sites of RAP and alpha(2)M*. A coreceptor of LRP, cell surface heparin sulfate proteoglycan, did not function in this system. Additional electrophysiology experiments demonstrated that the inhibitory potency of apoE[133-149] was threefold greater for NMDAR-transfected wild-type Chinese hamster ovary (CHO) cells compared with NMDAR-transfected CHO cells deficient in LRP. Studies with truncation and replacement variants of the apoE peptide demonstrated that the NMDAR inhibitory properties of these peptides correlate with their binding affinities for LRP. These novel results indicate that apoE functions as an inhibitor of NMDAR ion channels indirectly via LRP, and are suggestive of a participatory role for LRP in NMDAR-based neuropathies.

  17. 依靠信息化提升医院管理水平%Improving Hospital Management Level Relying on Informatization

    Institute of Scientific and Technical Information of China (English)

    王志华; 尚华; 李包罗

    2011-01-01

    The application of Hospital Information System is the important sign of a hospital ' s scientific managementand the key point of improving a hospital ' s management level. The paper studies the Hospital Information System ofTaiwan Chang Gung Memorial Hospital. Through the introduction of the organizational structure of Taiwan Chang GungMemorial Hospital ' s informatization team and the cases, in which they improve management level of their hospitalrelying on informatization, the paper analyses how to apply information system to improve a hospital ' s managementlevel.%医院信息系统的运用,是医院科学管理的重要标志,也是提升医院整体管理水平的关键着力点.以台湾长庚纪念医院信息系统为对象,通过介绍长庚医院信息化团队的组织架构及其依靠信息化提升医院管理水平的实例,分析如何通过信息化建设来提升医院管理水平.

  18. Static Enforcement of Static Separation-of-Duty Policies in Usage Control Authorization Models

    Science.gov (United States)

    Lu, Jianfeng; Li, Ruixuan; Hu, Jinwei; Xu, Dewu

    Separation-of-Duty (SoD) is a fundamental security principle for prevention of fraud and errors in computer security. It has been studied extensively in traditional access control models. However, the research of SoD policy in the recently proposed usage control (UCON) model has not been well studied. This paper formulates and studies the fundamental problem of static enforcement of static SoD (SSoD) policies in the context of UCONA, a sub-model of UCON only considering authorizations. Firstly, we define a set-based specification of SSoD policies, and the safety checking problem for SSoD in UCONA. Secondly, we study the problem of determining whether an SSoD policy is enforceable. Thirdly, we show that it is intractable (coNP-complete) to direct statically enforce SSoD policies in UCONA, while checking whether a UCONA state satisfies a set of static mutually exclusive attribute (SMEA) constraints is efficient, which provides a justification for using SMEA constraints to enforce SSoD policies. Finally, we introduce a indirect static enforcement for SSoD policies in UCONA. We show how to generate the least restrictive SMEA constraints for enforcing SSoD policies in UCONA, by using the attribute-level SSoD requirement as an intermediate step. The results are fundamental to understanding SSoD policies in UCON.

  19. An Expert System-Based Site Security Officer

    OpenAIRE

    Adeniran, Olusola; Ikuomola, Ronke; Sodiya, Adesina Simon

    2007-01-01

    A Site Security Officer (SSO) who is a network security staff that responds to alarms from an Intrusion Detection System (IDS), is always faced with the critical problem of low response time when the network becomes big. Even a skilled SSO is hard-pressed and less productive when collecting and analyzing IDS output manually as the frequency of intrusion increases. In this work, an Expert System-Based SSO (ExSSO) is designed to correct this problem. The design presents an architecture that enc...

  20. The 'French Fry' VAC technique: hybridisation of traditional open wound NPWT with closed incision NPWT.

    Science.gov (United States)

    Chopra, Karan; Tadisina, Kashyap K; Singh, Devinder P

    2016-04-01

    Surgical site occurrences (SSO), specifically surgical site infections represent a significant burden in the US health care system. It has been hypothesised that postoperative dressing can help drive down SSO. We describe the successful use of a novel technique combining both closed incision and open negative pressure wound therapy in the management of a high-risk wound associated with lymphoedema of obesity.

  1. System for calibration of instruments of x-ray measurement (CIR-X) applying the PGCS; Sistema para calibracion de instrumentos de medicion de rayos X (CIR-X) aplicando el PGCS

    Energy Technology Data Exchange (ETDEWEB)

    Gaytan G, E.; Rivero G, T.; Cruz E, P.; Tovar M, V.M.; Vergara M, F.J. [ININ, 52750 La Marquesa, Estado de Mexico (Mexico)]. e-mail: egg@nuclear.inin.mx

    2007-07-01

    The Department of Metrology of Ionizing Radiations of the ININ carries out calibration of instruments for X-ray measurement that determine the operation parameters in X-ray diagnostic machines of the health and private sectors. To facilitate this task, the Department of Automation and Instrumentation developed a system for acquisition and signals processing coming from a reference voltage divider with traceability at NIST that is connected directly to the X-rays tube. The system is integrated by the X-ray unit, the X-ray measurement equipment Dynalizer IIIU of RADCAL, a data acquisition card, a personal computer and the acquisition software and signals processing. (Author)

  2. Proceedings of the Ship Control Systems Symposium (7th) Held in Bath, England on 24-27 September 1981. Volume 1

    Science.gov (United States)

    1984-09-27

    fModernization Program ( TRiUMP ). It has taken the Canadian Navy almost seven years to get approval to develop SHINMACS and to award a contract for an...S W~LFL.JS~ ~St1EL -OA L - - - - - - - iIEPTI Zi 5 M0Mnhne rplinMcieyCnrlSse Test Handset Dn a ec t S, Dynale p lo R:<v~~~ vn s!-.. ti yr Elniirio Ac ...programmed in BASIC. The controller unit was completed by adding an ADO (0)D4) and a DAC (ZNS251). (figure 3). MICRO- STEERING ADC COMPUTER D] AC

  3. The Effects of IGFBP3 Induction by TFG-B in Breast Tumorigenesis

    Science.gov (United States)

    2000-09-01

    P. FREDERICK,’ LIHUA PAN ,2 ANITA J. BORTON,1 YUAN ZHUANG, 2 AND XIAO-FAN WANG’: Departnent of Pharmacology and Cancer Biologp’ and Department of...RNA wvere consisrmsed thtrouigh tse qatliifiihcaitiini isf tilse protection tusiisg lDynalseads- Moutse pans T ( Dynal. Eake Success. N.Y.) andt 01...Weinstein, G. S. Bova, W. B. Isaacs, P. Cairns, H. Nawroz, D. Sidransky, Decapentaplegic. Nature 388:304-308. R. A. Casero , P. S. Meltzer, S. A. Hahn, and S

  4. The Training of Language Aesthetic Taste Should Rely on the Application of Modern Educational Technology%语文审美情趣的培养要靠现代教育技术的应用

    Institute of Scientific and Technical Information of China (English)

    潘虹

    2012-01-01

    Language teaching materials in primary and secondary schools selected the essay,the subject matter is di-verse,rich in content,reflect all aspects of the language from a different angle and depth of aesthetic taste,to show stu-dents the extremely rich and wide range of aesthetic content.Modern educational technology with its convenient,ex-tremely rich,audio-visual blend staggered movement,such as infection and strong features and replaced by the text,sound,image in one of the advantages of traditional "textbooks + chalk + blackboard" teaching methods,teachers in-dispensable tool,more and more applied to the day-to-day classroom.It makes the perfect realization of the cultivation of aesthetic taste in language teaching.%中小学语文教材所选取的范文,题材多样、内容丰富,从不同角度和深度反映了语文各个方面的审美情趣,向学生展示了极其丰富和广泛的美学内容。现代教育技术凭着其方便快捷、声情并茂、视听交融、动静交错、感染力强等特点和集文字、声音、图像于一体的优势取代了传统的"课本+粉笔+黑板"的教学手段,成为教师不可缺少的工具,被越来越多应用到日常的课堂中去。它使语文教学中的审美情趣的培养得到了完美的实现。

  5. Internet: a negatividade do discurso da mídia versus a positividade da experiência pessoal. À qual dar crédito? Internet: Negative media discourse versus positive personal experience Which should we rely on?

    Directory of Open Access Journals (Sweden)

    Ana Maria Nicolaci-da-Costa

    2002-01-01

    Full Text Available Tendo em vista a insistência da mídia brasileira em divulgar resultados de pesquisas estrangeiras, principalmente norte-americanas, que sistematicamente apontam os efeitos negativos do uso intensivo da Internet, foi realizada uma pesquisa em profundidade com usuários "pesados" brasileiros. Foram minuciosamente entrevistados 20 homens e mulheres que se conectam diariamente à Rede durante no mínimo duas horas de seu tempo de lazer. O levantamento de seus hábitos, preferências e opiniões revela que esses usuários enfrentam um dilema de difícil solução. Por um lado, sequer cogitam se contrapor a um discurso que lhes é passado como científico -- e, portanto, confiável -- , mesmo quando este avalia o uso intensivo da Internet sob a ótica da patologia. Por outro, são instados por seu cotidiano, em cuja rotina a Internet se inseriu sem maiores problemas, a dar crédito à sua própria experiência positiva na Rede. Para resolver o problema, lançam mão de estratégias de distanciamento, relativização e até mesmo desqualificação bem-humorada do discurso da patologia divulgado pela mídia.Given Brazilian media's insistence in making public the results of foreign, mainly American, investigations that systematically point out the negative effects of the intensive use of the Internet, an in-depth research was conducted on Brazilian "heavy" users. Twenty men and women, who log on to the Net daily for at least two hours of their leisure time, were interviewed in detail. Knowledge of their habits, preferences and opinions reveals that these users are faced with a difficult dilemma. On the one hand, they do not dare criticize the negative discourse that reaches them through the media. They consider it trustworthy because of its alleged scientific bases. On the other hand, they are urged by their daily life, which has absorbed the Internet in its routine without any major problems, to give credit to their own positive experience on the Net. In order to solve the problem, they resort to strategies that distance themselves from what the media say and even disqualify it in a good-humoured manner.

  6. Research on hyperbaric oxygen therapy for relieing concurrent diseases after radiotherapy of cervix cancer%高压氧治疗缓解宫颈癌放疗后并发症研究

    Institute of Scientific and Technical Information of China (English)

    李启明; 李宁; 郑成位; 陈正堂

    2006-01-01

    目的 改善由宫颈癌盆腔放射引起的严重放射性直肠炎.方法 24例宫颈癌外照射或合并近距离照射后放射性直肠炎患者,经过药物及内窥镜治疗无效者,进行2.4个大气压100%氧气;90min治疗;每周治疗5~7次;治疗10~28个疗程.结果 高压氧治疗结束后89%(18例患者中16例)出血症状减轻或消失;71%(17例中12例)疼痛症状缓解或消失;62%(13例中8例)排便紧迫感减轻或消失.结论 高压氧治疗对于难治性放射性直肠炎有较高的有效率和耐受性是治疗这种常见、难治病例的重要选择.

  7. The GnRH receptor and the response of gonadotrope cells to GnRH pulse frequency code. A story of an atypical adaptation of cell function relying on a lack of receptor homologous desensitization.

    Directory of Open Access Journals (Sweden)

    Christian Bleux

    2010-01-01

    Full Text Available Brain control of the reproductive system is mediated through hypothalamic gonadotropin-releasing hormone (GnRH which activates specific receptors (GnRHR present at the surface of the pituitary gonadotropes to trigger secretion of the two gonadotropins LH and FSH. A unique feature of this system is the high dependence on the secretion mode of GnRH, which is basically pulsatile but undergoes considerable fluctuations in pulse frequency pattern in response to endogenous or external factors. How the physiological fluctuations of GnRH secretion that orchestrate normal reproduction are decoded by the gonadotrope cell machinery to ultimately control gonadotropin release and/or subunit gene transcription has been the subject of intensive studies during the past decades. Surprisingly, the mammalian GnRHR is unique among G protein-coupled receptor family as it lacks the carboxy-terminal tail usually involved in classical endocytotic process. Accordingly, it does not desensitize properly and internalizes very poorly. Both this atypical intrinsic property and post-receptor events may thus contribute to decode the GnRH signal. This includes the participation of a network of signaling pathways that differently respond to GnRH together with a growing amount of genes differentially sensitive to pulse frequency. Among these are two pairs of genes, the transcription factors EGR-1 and NAB, and the regulatory factors activin and follistatin, that function as intracellular autoregulatory feedback loops controlling respectively LHbeta and FSHbeta gene expression and hence, LH and FSH synthesis. Pituitary gonadotropes thus represent a unique model of cells functionally adapted to respond to a considerably fluctuating neuroendocrine stimulation, from short individual pulses to sustained GnRH as observed at the proestrus of ovarian cycle. Altogether, the data emphasize the adaptative reciprocal complementarity of hypothalamic GnRH neurones and pituitary gonadotropes to function as an original unit.

  8. 以"生长教学法"为依托促邓小平理论进学生头脑%Relying on the "Forming Teaching Method" and Impressing Deng Xiao ping's Theory on the Students' Mind

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    本文运用"生长教学法"教学思维,进行课教学改革和建设.提出了邓小平理论"进头脑"的教学目标,在课堂教学模式方面,实行了三段式教学,着力解决了邓小平理论"三进"工作中最关键的一环--进头脑工作.创设了"生成考核法",巩固和增强学生学习邓小平理论的学习效果.

  9. EL EBOOK BUSCA EN LA LECTURA SOCIAL LA PROPUESTA QUE IMPULSE NUEVOS FORMATOS DE ÉXITO/DIGITAL BOOKS RELY ON SOCIAL READING TO FIND NEW SUCCESS PROPOSALS AND FORMATS

    National Research Council Canada - National Science Library

    Berta García Orosa; Xosé López García

    2016-01-01

      Digital reading development has reached turning point. In view of the recent data, the edition of electronic books has dropped for the second consecutive year in 2014, while paper books production has increased (MECD, 2015...

  10. Promoteing the Western Underdeveloped Areas Leaping Development Relying on independent innovation%依靠自主创新推动西部欠发达地区跨越式发展

    Institute of Scientific and Technical Information of China (English)

    郑文

    2014-01-01

    The analysis of China's West owes the manifestations and causes of economic and technological advanced backward area , expounds the independent innovation to change the backward situation,the realization of the practical significance and the feasibility of development by leaps and bounds,and how the less developed western regions through independent innovation to achieve unconventional, leap forward development.%分析我国西部欠发达地区经济科技落后的表现及原因,阐述自主创新对于改变落后状况,实现跨越式发展的现实意义和可行性,以及西部欠发达地区如何通过自主创新实现超常规、跨越式发展。

  11. The Exploration and Thoughts on Development of Characteristic Tobacco Relying on the Original Ecological in Liangshan Area%凉山山地原生态特色烟叶开发实践与思考

    Institute of Scientific and Technical Information of China (English)

    杜如万; 戴培刚; 王剑; 王勇; 刘东阳; 董建新; 陈玉蓝; 胡海洲; 张玉

    2016-01-01

    Tobacco production is facing severe and complicated situation according to the idea of ecological consumption, green consumption and healthy consumption, it is urgent to adjust measures to local conditions and take the road of regional characteristics. Liangshan has the inherent advantages of developing high quality flue-cured tobacco leaf, and has made remarkable achievements in the construction of modern tobacco agriculture. Facing the new situation,Liangshan tobacco overcome challenges, seize the development opportunities, exploringand thinking the development of characteristics tobacco around the original ecological, aimed at the construction of theory system, technologysystem and culturesystem, what’s more, exploring the develop road of ecological, quality and safety for Liangshan tobacco. This paper summarizes the background, advantages, and task planning of the development of the original ecological tobacco leaf in Liangshan mountainous region, which provides reference for the national tobacco production.%随着生态消费、绿色消费、健康消费理念的日渐兴起,烟叶生产面临着严峻且复杂的形势,迫切要求因地制宜,走区域特色之路。凉山具有发展特色优质烟叶的先天优势,在现代烟草农业建设过程中取得了显著的成绩。面对新形势,凉山烟草直面市场挑战,抓住发展机遇,围绕山地原生态特色烟叶开发进行了深入的探索与思考,旨在构建凉山烟叶生产的理论、技术、文化三大体系,探索生态化、品质化、安全化的发展之路。概述了凉山山地原生态特色烟叶开发的背景、优势、总体思路和主要措施,为全国烟叶生产走区域特色之路提供参考。

  12. Relying on health education,doing a good job of women menopausal health care%依托健康教育,做好妇女更年期保健

    Institute of Scientific and Technical Information of China (English)

    于梅

    2015-01-01

    加强妇女更年期保健能够提高妇女的身体健康。加强更年期妇女生殖健康保健是妇产科学和全社会的重要任务。本文主要对妇女更年期保健的健康教育问题进行阐述。%Strengthening the women menopausal health care can improve the health of women.Strengthening menopause women reproductive health care is an important task for obstetrics and gynecology and the whole society.In this paper,the author mainly elaborates the health education problem of women menopausal health care.

  13. Salt shock-inducible Photosystem I cyclic electron transfer in Synechocystis PCC6803 relies on binding of ferredoxin : NADP(+) reductase to the thylakoid membranes via its CpcD phycobilisome-linker homologous N-terminal domain

    NARCIS (Netherlands)

    van Thor, JJ; Jeanjean, R; Havaux, M; Sjollema, KA; Joset, F; Hellingwerf, KJ; Matthijs, HCP

    2000-01-01

    Relative to ferredoxin:NADP(+) reductase (FNR) from chloroplasts, the comparable enzyme in cyanobacteria contains an additional 9 kDa domain at its amino-terninus, The domain is homologous to the phycocyanin associated linker polypeptide CpcD of the light harvesting phycobilisome antennae. The pheno

  14. «Université virtuelle française» à l'Université de Hiroshima : Essai de créer les cours de la langue française reliés à Internet

    OpenAIRE

    平手, 友彦

    2003-01-01

    Nous avons installe un site ≪Universite virtuelle frangaise≫ a l'Universite de Hiroshima. Ce site a pour but I'enseignement de la langue francaise par Internet. Dans ce contexte, le mot ≪Universite virtuelle≫ n'indique pas la simulation d'une universite reelle par des images de synthese tridimensionnelles, mais la possibilite d'acceder n'importe quand, depuis n'importe ou et aussi souvent qu'on le souhaite sur le site grace au WEB. Notre site se compose actuellement de quatre cours caracteris...

  15. Relying on "Three Centers" to Improve the Young Teachersˊ Teaching Ability%依托“三个中心”提升青年教师教学能力

    Institute of Scientific and Technical Information of China (English)

    张华瑞; 苟健; 戴立益

    2014-01-01

    教师是教育事业发展的基础,是提高高等教育质量、办好人民满意的高等教育的关键。华东师范大学立足学校现有资源,探索教师教学发展中心、教师教育实验教学中心和大学生学业发展指导中心的联动机制,有效提升青年教师教学能力,成为国内同类院校开展教师专业发展的重要借鉴。%As the basic guarantee of the educational development,teachers are the key factor to improve the quality of higher education and launch higher education that satisfies the needs of the people. Based on the existing resources,East China Normal University explores the linkage mechanism of teachersˊteaching center, experimental teaching center for teacher education and center for college studentsˊAcademic development guid-ance,effectively improves young teachersˊteaching ability,and it becomes an important reference for domes-tic universities and colleges to carry out teachersˊ professional development.

  16. L’incertitude dans l’utilisation des médias géolocalisés : étude de deux applications reliées à la mobilité urbaine

    Directory of Open Access Journals (Sweden)

    Julia Salles

    2015-12-01

    Full Text Available À partir de la différentiation entre les pratiques stratégiques et tactiques, développée par Michel de Certeau (1990, et du concept de l’espace hybride (De Souza et Silva, 2006 ; Nova, 2009, ce travail distinguera les différents rapports à l’incertitude lors du déplacement en milieu urbain par les utilisateurs des médias géolocalisés, notamment par l’étude de deux applications pour appareils mobiles, Foursquare/Swarm et Walking the Edit.

  17. 依托“两双基地”建设“两双师资”队伍探析%Analysis on The Construction of "Two-type Teachers" Team, Relying on the "Two-double Base"

    Institute of Scientific and Technical Information of China (English)

    罗星海

    2012-01-01

    Two-type quality and two-type teachers' team structure is the key issue to improve the teaching quality of higher vocational education. It is also a pressing matter of the moment for construction of two-type Teachers' team, the key point and long-term plan. The paper starts with analysis of situation and problems of higher vocational col- lege teachers team construction, puts forward that "two-double base" is the effective platform for construction of" two-type teacher" team. The paper discusses from solving teachers' quantity, structure, quality, ability, stability, and other aspects of the system, puts forward to further study and exploration of the problem.%双师素质和双师结构的“两双师资”队伍建设是提高高职教育教学质量的关键,是高职院校的当务之急、关键之点、长远之计。文章从分析高职学院师资队伍建设的现状与问题人手,提出了“两双基地“是建设“两双师资”队伍的有效平台,从解决教师队伍数量、结构、素质、能力、稳定性、制度等方面进行了阐述,提出了需进一步研究与探索的问题。

  18. 论广告生存与超越的根本——广告创意与消费心理%The Base Advertising Relies on and Transcends——Ad Conception and Consumer Psychology

    Institute of Scientific and Technical Information of China (English)

    喻珊; 吴东良; 侯立丽

    2007-01-01

    @@ 在人类文明的进程中,各种语言伴随着地域环境的不同经历着产生、发展与变革.但没有一种语言系统能像广告一样,呈现出持久和强大的生命力.社会经济的发展,人类文明的进步,一切人类的活动都离不开广告的作用.

  19. The Threshold of Protection from Liver-Stage Malaria Relies on a Fine Balance between the Number of Infected Hepatocytes and Effector CD8+ T Cells Present in the Liver

    Science.gov (United States)

    Longley, Rhea J.; Gola, Anita; Ulaszewska, Marta; Lambe, Teresa; Hill, Adrian V. S.

    2017-01-01

    Since the demonstration of sterile protection afforded by injection of irradiated sporozoites, CD8+ T cells have been shown to play a significant role in protection from liver-stage malaria. This is, however, dependent on the presence of an extremely high number of circulating effector cells, thought to be necessary to scan, locate, and kill infected hepatocytes in the short time that parasites are present in the liver. We used an adoptive transfer model to elucidate the kinetics of the effector CD8+ T cell response in the liver following Plasmodium berghei sporozoite challenge. Although effector CD8+ T cells require malaria is reliant on CD8+ T cells being able to locate infected hepatocytes, resulting in a protection threshold dependent on a fine balance between the number of infected hepatocytes and CD8+ T cells present in the liver. With such a fine balance determining protection, achieving a high number of CD8+ T cells will be critical to the success of a cell-mediated vaccine against liver-stage malaria. PMID:28087668

  20. Into the Curriculum. Art: Pueblo Storyteller Figures [and] Physical Education: Games That Rely on Feet [and] Reading/Language Arts: Movie Reviews [and] Reading/Language Arts: Reader's Choice [and] Science: Float or Sink [and] Social Studies: Buildings and Designs.

    Science.gov (United States)

    Crane, Jean; Rains, Annette

    1996-01-01

    Presents six curriculum guides for art, physical education, reading/language arts, science, and social studies. Each guide identifies library media skills objectives; curriculum objectives; grade levels; print and nonprint resources; instructional roles; the activity; and procedures for completion, evaluation, and follow-up activities. (AEF)

  1. Vatenfall relies on biomass. Short rotation plantations shall secure the energy supply of a thermal power station in Berlin; Vattenfall setzt auf Biomasse. Kurzumtriebsplantagen sollen Versorgung eines Berliner Heizkraftwerkes sichern

    Energy Technology Data Exchange (ETDEWEB)

    Biernath, Dieter

    2013-04-01

    The Vattenfall subsidiary Energy Crops (Hamburg, Federal Republic of Germany) looks for farmers for contract farming of short rotation plantations in order to secure the energy supply of a thermal power station in Berlin (Federal Republic of Germany). Energy Crops supports farmers with seedlings, equipment and consultancy. However, Energy Crops looks for the correct methods.

  2. Rely on the advantage, grasp the opportunity and speed up the development of Fujian modern fishery%立足优势抢抓机遇加快建设福建现代渔业

    Institute of Scientific and Technical Information of China (English)

    刘修德

    2011-01-01

    Under the premise of summarizing present development basis of provincial fishery, the paper analyzed the opportuni- ties and challenges which modem fishery construction are facing, pointing out the aims and methods to develop Fujian modem fishery, and put forward some countermeasures : greatly develop newly efficient fishery to augment new advantages of fishery de- velopment; reform the traditional fishery by using modem technology, so as to improve the fishery scientific and technical con- tent; establish development concept of wining success through quality and carry out the quality management in the production process; open in every aspect to the outside world, to promote fishery participation in international cooperation and competition on a broader scale, in greater depth and at a high level; intensive protect fishery resources to consolidate the foundation of contain- able development; implement the fishery policy of "supporting and benefiting fisherman", to guarantee the rights and interests of fisherman in line with the laws.%本文在总结当前全省渔业发展基础的前提下,分析了福建现代渔业建设面临的机遇和挑战,指出了福建现代渔业建设的目标和实现途径,提出了福建现代渔业建设的对策措施——大力发展新型高效渔业,增创渔业发展新优势;用现代科学技术改造传统渔业,提高渔业科技含量;培养以质取胜的发展理念,实施生产全过程质量管理;全方位扩大对外开放,促进渔业在更大范围、更广领域、更高层次上参与国际竞争与合作;加强渔业资源养护,巩固可持续发展的基础;贯彻落实支渔惠渔政策,依法保障渔民合法权益。

  3. 依托课题研究,提升园本特色的有效性策略研究%Research on Effective Strategies of Improving Kinder-garten-based Characteristics Relying on Subject Study

    Institute of Scientific and Technical Information of China (English)

    林宁宁

    2014-01-01

    Doing and playing activity is the kindergarten-based characteristics of Qixunkou Kindergarten. For a long time, start-ing from the traditional doing and playing activity, our kinder-garten has been deepening the exploration on it, gradually refin-ing and strengthening it, sustainably and deeply developing the educational value of doing and playing, so as to broaden our thinking and make it bigger and stronger. In the kinder-garten-based characteristic research on doing and playing in our kindergarten, we adhere to two footholds, one is the game as the carrier and the other is children's development as the goal. In the nearly 20 years of research and discussion on kindergarten-based characteristic development, we regard children's development as the goal, pay attention to case studies, and continuously explore effective strategies of promoting the kindergarten-based sustain-able development.%做做玩玩活动是旗汛口幼儿园的园本特色。长期以来,我园从传统的“做做玩玩”活动入手,深入探索,逐步细化、强化,持续、深入地挖掘“做做玩玩”的教育价值,拓宽思路,使之做强做大。我园的做做玩玩园本特色研究坚持两个立足点,一是以游戏为载体,二是以幼儿发展为目标。在幼儿园长达近20年的园本特色发展研讨中,我们以幼儿发展为目标,关注案例研究,不断探索促进园本特色可持续发展的有效策略。

  4. Can mothers rely on the Brazilian health system for their deliveries? An assessment of use of the public system and out-of-pocket expenditure in the 2004 Pelotas Birth Cohort Study, Brazil

    Directory of Open Access Journals (Sweden)

    Bertoldi Andréa D

    2008-03-01

    Full Text Available Abstract Background In a country where comprehensive free health care is provided via a public health system (SUS, an unexpected high frequency of catastrophic out-of-pocket expenditure has been described. We studied how deliveries were financed among mothers of a birth cohort and whether they were an important source of household out-of-pocket expenditure. Methods All deliveries occurring in the city of Pelotas, Brazil, during 2004, were recruited for a birth cohort study. All mothers were interviewed just after birth and three months later. Comprehensive data on the pregnancy, delivery, birth conditions and newborn health were collected, along with detailed information on expenses related to the delivery. Results The majority of the deliveries (81% were financed by the public health system, a proportion that increased to more than 95% among the 40% poorest mothers. Less than 1% of these mothers reported some out-of-pocket expenditure. Even among those mothers covered by a private health plan, nearly 50% of births were financed by the SUS. Among the 20% richest, a third of the deliveries were paid by the SUS, 50% by private health plans and 17% by direct payment. Conclusion The public health system offered services in quantity and quality enough to attract even beneficiaries of private health plans and spared mothers from the poorest strata of the population of practically any expense.

  5. 依靠国际化经营快速发展的马来西亚国家石油公司%Petronas: Relies on Internationalized Management for Rapid Development

    Institute of Scientific and Technical Information of China (English)

    朱和

    2001-01-01

    经过近10年的努力,在政府的支持下,马来西亚国家石油公司(Petronas)已发展成为亚洲地区为数不多的国际化经营搞得最好的一体化综合性石油公司之一.在2000年度Petronas的营业收入中,国内部分只占20.3%,出口占48.4%,国际化经营部分占31.3%.目前,Petronas已经在全球26个国家或地区开展了国际合作业务.Petronas国际化经营有几个特点:(1)广泛合作,以亚洲国家为主开展国际化经营;(2)扬长避短,突出和扩展优势业务;(3)合资为主,"请进来"与"走出去"兼而有之,以降低风险;(4)以卓有成效的国际化经营带动公司整体业务的迅速发展.Petronas国际化经营的成功得益于政府的鼎力支持,优越的地理位置,对外合作的悠久历史,以灵活方式合理规避风险以及重视企业形象宣传,广交朋友,沟通信息等.

  6. Thinking about Promoting People's Livelihood Development Relying on Information Construction%关于依托信息化建设促进民生发展的思考

    Institute of Scientific and Technical Information of China (English)

    许曼

    2012-01-01

    In the contemporary development way, information construction and the people's livelihood development are hot social concem. This artide from the concept of people's livelihood, in the informatization construction and discussed based on the relation of the people's livelihood, and further promote the development of informatization construction of people's livelihood the approach of thinking and described.%在当代,信息化建设和民生发展都是备受关注的社会热点问题。文章从民生的概念谈起,在探讨信息化建设与民生关系的基础上,进一步对信息化建设促进民生发展的途径进行了思考和阐述。

  7. Relying on the"Internet plus"to construct a new mode of food safety supervision%依托“互联网+”构建食品安全监管新模式

    Institute of Scientific and Technical Information of China (English)

    赵燕

    2016-01-01

    With the transformation and upgrading of the Internet era of food industry in China,and puts forward new requirements for food safety supervision work. The traditional manual supervision mode is restricted by the method of technical means and methods,there are a lot of security loopholes. Must be leveraging "Internet plus" way of thinking and technical means,through the development and application of information technology the platform,accelerate the construction of food safety electronic supervision system,strengthen the food safety traceability management and whole process supervision,construction supervision model of big data,the establishment of government regulation and public supervision integrated social supervision system of governance,so as to realize the full and fine food safety management,to create a new model of food industry safety control.%随着互联网时代我国食品工业的转型升级,对食品安全监管工作也提出了新的要求。传统的人工监管模式受到技术手段和方式方法的制约,存在大量安全监管漏洞。必须借力“互联网+”的思维方式和技术手段,通过信息化平台的开发与应用,加快推进食品安全电子监管系统建设,强化食品安全溯源管理和全流程监管,构建大数据监管模型,建立政府监管和社会监督有机结合的社会共治监管体系,从而实现食品安全管控的全程化和精细化,开创食品产业安全管控新模式。

  8. Recovering marine resources can not only rely on hot season fishing off%单纯依靠休渔难以摆脱资源危急的厄运

    Institute of Scientific and Technical Information of China (English)

    王耀富

    2000-01-01

    @@ 在百年交替、千年更迭的世纪之交,我国海洋渔业成功地实施了新的休渔制度,不仅使我国海洋渔业资源保护措施日臻完善,也使我国海洋渔业资源管理水平,跨越了一个新的高度.

  9. 关于依靠微信进行图书馆服务推广的研究%Study of rely on WeChat for library services

    Institute of Scientific and Technical Information of China (English)

    程雅倩

    2015-01-01

    本论文研究了高校图书馆通过微信建立交流的方法,国内很多高校图书馆已经提出了将微信作为一种营销工具的方式。本文研究了图书馆服务推广的现状和问题,探讨了微信在图书馆推广服务中能够起到的作用,并对如何更好的使用微信提出了对策。%This paper studied the university library through WeChat establish communication method, the domestic many university libraries have put forward the WeChat as a way of marketing tool. This paper studies the present situation and problems of the library service promotion, this paper discusses the WeChat can play a role in promotion service in the library, and how to better use WeChat countermeasures are put forward.

  10. How to promote the Integrated Marketing Communications relying on web2.0%基于web2.0的整合营销传播策略探讨——以新浪个案为例

    Institute of Scientific and Technical Information of China (English)

    张艳

    2007-01-01

    web2.0的兴起引发了传播形态、媒体环境及营销手段等种种变化,这些均为整合营销传播的实施提出了新的契机,关键在于我们能否充分发挥web2.0的应用元素,推进整合营销传播的执行.本文针对这一问题结合典型案例分析,提出了三点可操作性策略分析.

  11. Establishment of Breast Cancer Screening Mechanism Relied on Service System for Women and Children Health Care%依托妇幼卫生服务体系建立乳腺癌筛查机制

    Institute of Scientific and Technical Information of China (English)

    杨蓉; 付泽鸿; 张斌; 杨少萍; 张丹; 李玉霞

    2012-01-01

    Objective To explore the effects of service system for women and children health care on breast cancer screening. Methods A random cluster sampling was taken in the central districts of Wuhan, and 33 019 women within 35~59 years old were chosen to be study samples. Servise system for women and children health care was utilized to organize and manage breast cancer screening. Breast cancer screening method was carried out by combination of clinic breast examination with molybdenum target X-ray and high-frequency ultrasound examination. All lesions and prognosis were determined by pathology. Screening rates,review rates,breast cancer detection rate were used to evaluate the effect of service system for women and children health care on breast cancer screening. Results 30 478 of 33 019 women participated the breast cancer screening. The screen rate was 92.30 %. The rates of screening molybdenum target and ultrasound examination was 92. 47 %0. All patients who were diagnosed as breast cancer or suspected cancer through combination of clinical examination,molybdenum target X-ray and ultrasound underwent surgery. 25 of women were diagnosed as breast cancer by pathology. The detection rate of the breast cancer screening was 82.03/100 000. Conclusion Depending on the breast cancer screening process organized by health service system for women and children care, the adherence to screening in women was increased and the medical technology was integrated better, which greatly enhanced the quality of breast cancer screening%目的 探讨妇幼卫生服务网络在乳腺癌筛查中的作用.方法 采取随机整群抽样的方法,在武汉市中心城区抽取35~59岁妇女33019名为研究对象,利用妇幼卫生服务网络对筛查工作进行组织管理,采用乳腺临床体检、钼靶X线摄片和彩超检查相结合的筛查方案,所有病变诊断及转归的判定均以组织病理学检查为依据.计算筛查率、复查率和乳腺癌检出率等指标,评价妇幼卫生服务体系对乳腺癌筛查的作用.结果 33019名妇女中,30478名妇女参与了乳腺癌筛查,筛查率为92.30%;钼靶或彩超的复查率为92.47%.通过临床检查、钼靶和彩超联合检查的乳腺癌或可疑癌患者均接受手术治疗,最终25人确诊为乳腺癌,乳腺癌检出率为82.03/100 000.结论 利用妇幼卫生服务网络组织乳腺癌筛查,提高了人群顺应性,技术力量能够得到整合,大大提高乳腺癌筛查的质量.

  12. Salt shock-inducible Photosystem I cyclic electron transfer in Synechocystis PCC6803 relies on binding of ferredoxin : NADP(+) reductase to the thylakoid membranes via its CpcD phycobilisome-linker homologous N-terminal domain

    NARCIS (Netherlands)

    van Thor, JJ; Jeanjean, R; Havaux, M; Sjollema, KA; Joset, F; Hellingwerf, KJ; Matthijs, HCP

    2000-01-01

    Relative to ferredoxin:NADP(+) reductase (FNR) from chloroplasts, the comparable enzyme in cyanobacteria contains an additional 9 kDa domain at its amino-terninus, The domain is homologous to the phycocyanin associated linker polypeptide CpcD of the light harvesting phycobilisome antennae. The pheno

  13. Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3´-Translational Enhancer that Communicates with the Corresponding 5´-Region through a Long-Distance RNA-RNA Interaction.

    Science.gov (United States)

    Blanco-Pérez, Marta; Pérez-Cañamás, Miryam; Ruiz, Leticia; Hernández, Carmen

    2016-01-01

    Cap-independent translational enhancers (CITEs) have been identified at the 3´-terminal regions of distinct plant positive-strand RNA viruses belonging to families Tombusviridae and Luteoviridae. On the bases of their structural and/or functional requirements, at least six classes of CITEs have been defined whose distribution does not correlate with taxonomy. The so-called TED class has been relatively under-studied and its functionality only confirmed in the case of Satellite tobacco necrosis virus, a parasitic subviral agent. The 3´-untranslated region of the monopartite genome of Pelargonium line pattern virus (PLPV), the recommended type member of a tentative new genus (Pelarspovirus) in the family Tombusviridae, was predicted to contain a TED-like CITE. Similar CITEs can be anticipated in some other related viruses though none has been experimentally verified. Here, in the first place, we have performed a reassessment of the structure of the putative PLPV-TED through in silico predictions and in vitro SHAPE analysis with the full-length PLPV genome, which has indicated that the presumed TED element is larger than previously proposed. The extended conformation of the TED is strongly supported by the pattern of natural sequence variation, thus providing comparative structural evidence in support of the structural data obtained by in silico and in vitro approaches. Next, we have obtained experimental evidence demonstrating the in vivo activity of the PLPV-TED in the genomic (g) RNA, and also in the subgenomic (sg) RNA that the virus produces to express 3´-proximal genes. Besides other structural features, the results have highlighted the key role of long-distance kissing-loop interactions between the 3´-CITE and 5´-proximal hairpins for gRNA and sgRNA translation. Bioassays of CITE mutants have confirmed the importance of the identified 5´-3´ RNA communication for viral infectivity and, moreover, have underlined the strong evolutionary constraints that may operate on genome stretches with both regulatory and coding functions.

  14. Survey on prevalence rate of healthcare-associated infections in hospitalized patients relying on informatization%依托信息化进行医院感染现患率调查

    Institute of Scientific and Technical Information of China (English)

    牟霞; 徐艳; 张骥; 王翀; 刘玮; 陈京; 杨怀

    2014-01-01

    OBJECTIVE To understand the basic distribution of healthcare-associated infections and its characteristics ,improve the quality of management of healthcare-associated infections and provide a scientific basis for prevention and control of the healthcare associated infections .METHODS The healthcare associated infections were diagnosed according to the criteria for diagnosis of nosocomial infections issued by the Minister of Health . Surveys were made by means of information system of hospital-associated infection management .Through the software for real-time monitoring of healthcare-associated infections ,the patients of early warning were found out by bed .The data were analyzed by SPSS13 .0 software .RESULTS The expected number of the survey was 2186 , and the actual number of the surveys was 2186 . Actually 100% of the selected patients took part in the investigation .The number of healthcare-associated infections was 86 patients with prevalence rate of 3 .93% ,and 122 cases with prevalence rate of 5 .58% .The top highest healthcare-associated infection rates were 47 .62% , 26 .67% and 17 .65% in ICU , hematology department and neurosurgery department respectively . The main infection site of healthcare-associated infection was lower respiratory tract accounting for 46 .72% .The usage rate of antibiotics was 50 .24% and the pathogenic detection rate of antibiotics users was 40 .38% .The usage rate of restricted antibiotics was 22 .22% with detection rate of 43 .48% .The usage rate of special antibiotics was 3 .38%with detection rate of 57 .14% .The top three pathogens causing health-associated infection were gram negative bacteria including Klebsiella pneumonia ,Acinetobacter baumannii and Pseudomonas aeruginosa accounting for 20 .45% ,18 .20% and 13 .93% respectively . It costed merely 12 hours to investigate the prevalence rate . CONCLUSION Prevalence rate survey of healthcare-associated infection by means of informatization can significantly improve the efficiency of healthcare-associated infection management .In order to promote the development of healthcare-associated infection management ,Informatization construction should be given enough attension .%目的:了解医院感染的分布状况及其特点,提高医院感染管理质量,为医院感染的预防与控制提供科学依据。方法依据原卫生部《医院感染诊断标准》,利用信息化手段,通过医院感染实时监控软件逐床查阅预警病例,进行医院感染现患率调查,数据应用SPSS13.0软件进行统计分析。结果应监测患者2186例,实际监测2186例,实查率为100.00%;发生医院感染患者86例、122例次,医院感染现患率为3.93%、例次感染率为5.58%;IC U、血液科、神经外科医院感染率最高,分别为47.62%、26.67%、17.65%,医院感染部位分布以下呼吸道为主,占46.72%;抗菌药物使用率为50.24%,抗菌药物使用者病原学送检率为40.38%;限制类抗菌药物使用率22.22%,送检率43.48%;特殊类抗菌药物使用率3.38%,送检率57.14%;引起医院感染的前3位病原菌均为革兰阴性菌,分别为肺炎克雷伯菌、鲍氏不动杆菌、铜绿假单胞菌,分别占20.45%、18.20%、13.93%;医院感染现患率调查时间仅用12 h。结论依托信息化手段进行医院感染现患率调查,显著提高医院感染管理效率,应给予医院感染管理信息化建设足够的重视,使医院感染管理更加完善。

  15. 依托EAM信息平台实现一流的设备维修中心%Rely on the information platform realize a first level rebuilding center

    Institute of Scientific and Technical Information of China (English)

    冯亚东

    2004-01-01

    企业资产管理系统简称EAM(Enterprise Asset Management),是一个面向资产密集型企业的信息化解决方案的总称.通过企业资产管理系统在设备大修中的科学应用,规范了维修过程,提高了管理质量,实现了设备大修全过程项目管理.

  16. 依托无线物联网技术的实时医疗健康监测系统%Real-time Health Monitoring System Relying on Wireless IOT Technology

    Institute of Scientific and Technical Information of China (English)

    杨龙频; 周林; 陈阳阳; 杨龙麟

    2013-01-01

    以国家自然科学基金项目“基于物联网技术的呼吸、脉搏异变及跌落的实时监测与报警的关键技术研究”为背景,提出基于无线传感器网络支持WiFi/3G/LTE协议标准中继传感节点构建的一个无处不在的实时医疗健康监测系统。在项目研究中,可穿戴式医疗传感设备是用来测量人类生命体征(如温度、血压、跌倒监测等),设备中内置的无线通信模块通过公用无线通信网络定时传输到实时医疗监测及分析系统。本文研究的架构是借助无线通信网络技术和优势,采用无线中继低功耗技术,自动计算最短路径进行测量数据传输,提高传输效率,降低功耗影响。%Based on the National Natural Science Fund Project which is Key technology problem research for drop and vital signs monitoring and real-time alarm system based on IoT (number 61171190) as the background. An advanced ubiquitous healthcare monitoring system is proposed with the aid of wireless sensor network based on the WiFi/3G/LTE relay sensor nodes. In this work, wearable healthcare devices are used to measure the human body vital signs (for example: Temperature, pressure, fall monitoring), Device built-in wireless communication module via a public wireless communication network to transmit real-time medical monitoring and analysis system. Our propose to set up framework through a wireless communication network technologies and the advantages of using low-power radio relay technology to automatically calculate the shortest path measurement data to improve the transmission efficiency, lower power consumption impact.

  17. IN THE AZERBAIJAN FIELD TURKISH LITERATURE, THE FIRST PERIODICAL PUBLICATIONS AND “MOLLA NESREDDIN DERGISI” / AZERBAYCAN SAHASI TÜRK EDEBIYATINDA ILK SÜRELI YAYIN FAALIYETLERI VE “MOLLA NESREDDIN DERGISI”

    Directory of Open Access Journals (Sweden)

    Dr. Mithat DURMUŞ

    2008-12-01

    Full Text Available In the Azerbaijan Field Turkish Literature, the firstperiodical publication studies started at the end of the19th century. This date includes the same period for theTurkish Field Turkish Literature. In this study, in whichlevel the periodical publication activities at bothgeography are and through which aims the experiencesare realized are tried to be explained. It is given greatimportance to the “Molla Nesreddin” magazine which waspublished at the and of the 19th century and at thebeginning of the 20th century and which is an greatexample for the Azerbaijan Field Turkish Literature.

  18. Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3´-Translational Enhancer that Communicates with the Corresponding 5´-Region through a Long-Distance RNA-RNA Interaction.

    Directory of Open Access Journals (Sweden)

    Marta Blanco-Pérez

    Full Text Available Cap-independent translational enhancers (CITEs have been identified at the 3´-terminal regions of distinct plant positive-strand RNA viruses belonging to families Tombusviridae and Luteoviridae. On the bases of their structural and/or functional requirements, at least six classes of CITEs have been defined whose distribution does not correlate with taxonomy. The so-called TED class has been relatively under-studied and its functionality only confirmed in the case of Satellite tobacco necrosis virus, a parasitic subviral agent. The 3´-untranslated region of the monopartite genome of Pelargonium line pattern virus (PLPV, the recommended type member of a tentative new genus (Pelarspovirus in the family Tombusviridae, was predicted to contain a TED-like CITE. Similar CITEs can be anticipated in some other related viruses though none has been experimentally verified. Here, in the first place, we have performed a reassessment of the structure of the putative PLPV-TED through in silico predictions and in vitro SHAPE analysis with the full-length PLPV genome, which has indicated that the presumed TED element is larger than previously proposed. The extended conformation of the TED is strongly supported by the pattern of natural sequence variation, thus providing comparative structural evidence in support of the structural data obtained by in silico and in vitro approaches. Next, we have obtained experimental evidence demonstrating the in vivo activity of the PLPV-TED in the genomic (g RNA, and also in the subgenomic (sg RNA that the virus produces to express 3´-proximal genes. Besides other structural features, the results have highlighted the key role of long-distance kissing-loop interactions between the 3´-CITE and 5´-proximal hairpins for gRNA and sgRNA translation. Bioassays of CITE mutants have confirmed the importance of the identified 5´-3´ RNA communication for viral infectivity and, moreover, have underlined the strong evolutionary constraints that may operate on genome stretches with both regulatory and coding functions.

  19. Specificities and pH profiles of adenine and hypoxanthine-guanine-xanthine phosphoribosyltransferases (nucleotide synthases) of the thermoacidophile archaeon Sulfolobus solfataricus

    DEFF Research Database (Denmark)

    Hansen, Michael Riis; Jensen, Kristine Steen; Rasmussen, Mads Skytte

    2014-01-01

    Two open reading frames in the genome of Sulfolobus solfataricus (SSO2341 and SSO2424) were cloned and expressed in E. coli. The protein products were purified and their enzymatic activity characterized. Although SSO2341 was annotated as a gene (gpT-1) encoding a 6-oxopurine phosphoribosyltransfe......Two open reading frames in the genome of Sulfolobus solfataricus (SSO2341 and SSO2424) were cloned and expressed in E. coli. The protein products were purified and their enzymatic activity characterized. Although SSO2341 was annotated as a gene (gpT-1) encoding a 6-oxopurine...... phosphoribosyltransferase (PRTase), the protein product turned out to be a PRTase highly specific for adenine and we suggest that the reading frame should be renamed apT. The other reading frame SSO2424 (gpT-2) proved to be a true 6-oxopurine PRTase active with hypoxanthine, xanthine and guanine as substrates, and we...... suggest that the gene should be renamed gpT. Both enzymes exhibited unusual profiles of activity versus pH. The adenine PRTase showed the highest activity at pH 7.5-8.5, but had a distinct peak of activity also at pH 4.5. The 6-oxo PRTase showed maximal activity with hypoxanthine and guanine around pH 4...

  20. Survey Tingkat Penggunaan Single Sign On pada 500 Situs Peringkat Teratas Alexa.com

    Directory of Open Access Journals (Sweden)

    I Wayan Manik Suhartanta

    2017-04-01

    Full Text Available Advances in Internet technology has provided a new market for the growth of the service provider. New sites appear in large numbers every year. This gives a new problem for users of the service. Users are forced to memorize many username and password to access the service. Overcoming it is a new emerging technology, the technology called Single Sign On (SSO. SSO offers simple authentication process for users to access many services sites. But the level of confidence of users and service providers, in the use of SSO technology have not been investigated. In this research shows that the rate of utilizing the SSO technology of the top 500 sites on alexa.com by 31% (155 websites. And the level of utilization of the SSO more than one provider, by 41%. In studies it appears that the most used SSO services provider is google followed by facebook and twitter. (turnitin check 9% Keyword— Single Sign On (SSO, Computer Security, Authentication DOI: 10.24843/MITE.1601.07

  1. Shoreline type and subsurface oil persistence in the Exon Valdez spill zone of Prince William Sound, Alaska

    Energy Technology Data Exchange (ETDEWEB)

    Page, D.S. [Bowdoin College, Brunswick, ME (United States). Dept. of Chemistry; Boehm, P.D. [Exponent Inc., Maynard, MA (United States); Neff, J.M. [Neff and Associates, Duxbury, MA (United States)

    2008-07-01

    The grounding of the Exxon Valdez in Prince William Sound (PWS), Alaska in the spring of 1989 resulted in the release of 258,000 barrels of Alaska North Slope crude oil into the marine environment. Nearly 800 km of shoreline were oiled to some degree. There was an unprecedented oil spill cleanup effort following the spill. The shoreline surveys of the spill zone were synthesized in this paper in an effort to demonstrate the relationship between shoreline type and persistence of subsurface oil (SSO) residues. Shoreline surveys of surface and SSO indicate rapid initial oil loss with a decline from about 800 linear km of PWS shoreline in 1989 to about 10 km of oiled shoreline in 1992. The period of rapid loss was attributed to natural physical process, biodegradation and cleanup activities that removed accessible spill remnants from shorelines. This was followed by a slower natural average loss rate for less accessible surface and SSO deposits of about 22 per cent per year for the period 1992-2001. This paper emphasized that shoreline type plays a key role in determining SSO persistence. The geology of PWS is complex. Many of the shorelines where SSO persists have armouring layers composed of hard, dense clasts, such as the quartzite boulders and cobblestones that can protect SSO deposits. Eighteen years after the spill, persistent SSO deposits in PWS shorelines remain protected from tidal water-washing and biodegradation by a surface boulder/cobble armour and low sediment porosity. The SSO deposits are in a physical/chemical form and location where they do not pose a health risk to intertidal biological communities and animals. The surveys continue to substantiate that remaining SSO deposits in PWS continue to degrade and go away slowly. 37 refs., 5 tabs., 7 figs.

  2. Q/SdR.04.896-2008 SQR7130A217/Model A Car

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    1.Background As the development of global economy demands an increasingly large quantity of energy,the energy crisis is getting more severe.Auto manufacturers in Japan,America,and Europe have all began to develop hybrid power vehicles or its technology platform since 1990s,so as to save energy.Relied on its THS system which consists of the planet wheel and e-CVT,TOYOTA developed a series of strong hybrid power vehicles,such as Prius,Lexus,Highlander,Camry,etc.Based on its IMA system,HONDA developed a series of mild hybrid power vehicles,such as Insight,Civic,Accord,etc.On the basis of its Belt-driven Starter Generator (BSG) system,French SPA Group developed a series of weak hybrid power vehicles,such as Citroen C3,Citroen C2,Citroen 1007,Citroen 207,etc.In America the research of hybrid power vehicle is being done actively by a group of relevant parties consisting of Department of Transport,Department of Defense,major auto companies and relevant organizations under the leadership of Department of Energy and with the large quantity ofinvestment from GE Motors Co.and CHRYSLER.

  3. effects of temperature on the physicochemical properties of ...

    African Journals Online (AJOL)

    dell

    traditionally processed vegetable oils deteriorate significantly at elevated temperatures (p < 0.05). ... whereby the formulations of palm oil (PO) and sesame oil (SSO) appeared to be more stable than ... substantial production of oil from sesame.

  4. Space shuttle with common fuel tank for liquid rocket booster and main engines (supertanker space shuttle)

    Science.gov (United States)

    Thorpe, Douglas G.

    1991-01-01

    An operation and schedule enhancement is shown that replaces the four-body cluster (Space Shuttle Orbiter (SSO), external tank, and two solid rocket boosters) with a simpler two-body cluster (SSO and liquid rocket booster/external tank). At staging velocity, the booster unit (liquid-fueled booster engines and vehicle support structure) is jettisoned while the remaining SSO and supertank continues on to orbit. The simpler two-bodied cluster reduces the processing and stack time until SSO mate from 57 days (for the solid rocket booster) to 20 days (for the liquid rocket booster). The areas in which liquid booster systems are superior to solid rocket boosters are discussed. Alternative and future generation vehicles are reviewed to reveal greater performance and operations enhancements with more modifications to the current methods of propulsion design philosophy, e.g., combined cycle engines, and concentric propellant tanks.

  5. 76 FR 28036 - Request for Comments and Announcement of Workshop on Standard-Setting Issues

    Science.gov (United States)

    2011-05-13

    ... demand a royalty that reflects not only the ex ante market value of the patented invention, but also... provide more definition of RAND? Absent an SSO's definition or express limitations given by the...

  6. The Rules of Standard Setting Organizations: an Empirical Analysis

    OpenAIRE

    Chiao, Benjamin; Lerner, Josh; Tirole, Jean

    2006-01-01

    This paper empirically explores the procedures employed by standard-setting organizations. Consistent with Lerner-Tirole (2004), we find (a) a negative relationship between the extent to which an SSO is oriented to technology sponsors and the concession level required of sponsors and (b) a positive correlation between the sponsor-friendliness of the selected SSO and the quality of the standard. We also develop and test two extensions of the earlier model: the presence of provisions mandating ...

  7. Hybrid Films Based on a Bridged Silsesquioxane Doped with Goethite and Montmorillonite Nanoparticles as Sorbents of Wastewater Contaminants

    Directory of Open Access Journals (Sweden)

    Carolina V. Waiman

    2016-01-01

    Full Text Available The synthesis and characterization of silsesquioxane (SSO films with pendant dodecyl groups and doped with goethite (Gt or montmorillonite (MMT nanoparticles were carried out and the new materials tested as sorbents of diverse contaminants. The synthetic method used yielded SSO films with the inorganic substrates homogeneously distributed within the polymeric matrices. The new materials were characterized by SEM, FTIR, XRD, and DSC and tested to evaluate their capability for adsorbing metallic cations, organic dyes, and phosphate, frequent contaminants of industrial effluents. All films were found suitable for removing metallic cations. Results also showed that the SSO films undoped and doped with Gt are primarily apt for anionic compounds removal. Although the SSO films doped with MMT are capable of removing cationic contaminants from aqueous samples, the stiffness of the SSO matrix hinders MMT properties as an adsorbent. The possibility of dispersing nanoparticulate systems in the stable and chemically inert SSO matrices simplifies their application for contaminant removal, particularly because it makes the separation process of the absorbed pollutant from the treated medium easier.

  8. Efficient and Secure Single Sign on Mechanism for Distributed Network

    Directory of Open Access Journals (Sweden)

    Madhavi A. Indalkar

    2014-07-01

    Full Text Available Distributed network act as core part to access the various services which are available in the network. But the security related to distributed network is main concern. In this paper single sign-on SSO mechanism is introduced which gives access to all services by allowing to sign on only once by users. In this mechanism once user logs in to the Trusted Authority Center TAC then application or services which are register to trusted center will automatically verifies the user’s credentials details and these credentials like password or digital signature will be only one for all applications or services. Unlike all other previous mechanisms where in, if user wants to have access multiple services then for every service distinct user credentials (username, password must be required. SSO act as single authentication window to user for admittance multiple service providers in networks. Previously introduced technique based SSO technology proved to be secure over well-designed SSO system, but fails to provide security during communication. So here emphasis is given on authentication as open problem and on to refining the already proposed SSO process. And to do this along with RSA algorithm which was used in previous SSO process, we will be using MAC algorithm, which is intended to provide secured pathway for communication over distributed network.TAC i.e. Trusted Authority Center is used for sending token integrated with private and shared public key to user. Keywords -

  9. Manufacture of low-benzo(a)pyrene sesame seed (Sesamum indicum L.) oil using a self-designed apparatus.

    Science.gov (United States)

    Yi, Ji Yoon; Kim, Hui Ju; Chung, Myong-Soo

    2017-01-01

    The aim of this study was to lower benzo(a)pyrene (BaP) contents in sesame seed oil (SSO) during manufacture by using a self-designed apparatus, to determine its optimal conditions, and to analyze antioxidants in SSO which might be related to BaP content reduction. Washing and spin-drying steps reduce exogenous BaP contamination, and the reduced moisture in seeds lowered BaP content in final SSO. A ventilation system in the roasting step inhibits BaP formation and reabsorption, followed by a controlled compression step. The optimal condition, a single washing cycle with 2-min spin-drying, 1350-rpm ventilation, and a single compression cycle, reduced the BaP content in SSO to 2.93 μg/kg, where the raw seeds had been spiked with 10-μg/kg BaP. Total phenolic contents showed a reversal pattern to the distribution of BaP contents. Sesamol and sesamolin were quantified by a high performance liquid chromatography-ultraviolet detector, and it was suggested that sesamol which is a strong antioxidant might have prevented BaP formation during the roasting step. This study enabled the commercial production of low-BaP SSO, and the data could be used in further investigations of the BaP content reduction mechanism with quantitative chemical analysis of the SSO composition.

  10. trans-Fatty acid isomers in two sesame (Sesamum indicum L.) seed byproducts under processing.

    Science.gov (United States)

    Dhibi, Madiha; Mechri, Beligh; Cheraif, Imed; Hammami, Mohamed

    2010-12-08

    The present study has been inspired by the growing need for rigorously controlling the nutritional quality and safety of food products. The impact of application in the food industry on fatty acids composition, trans-fatty acids (TFAs), and conjugated linoleic acid (CLA) profiles were investigated in a highly consumed candy byproduct of sesame seed (chamia) in comparison to fresh sesame seed oil (SSO) and heated SSO under simulated frying experiments. The effect of treatment on SSO was studied by determining the TFA and CLA changes. Results showed significant differences between the two byproducts in TFA and CLA amounts. Total TFAs were found to be significantly higher in chamia than fresh SSO (1.31 versus 0.066%, respectively; p < 0.05) and even higher than all heated SSO from 2 to 10 h at 180 °C (1.31 versus 0.33%, respectively; p < 0.05). A significant linear relationship was found between trans-monounsaturated fatty acid (MUFA), trans-polyunsaturated fatty acid (PUFA), and total TFA and the time of processing, with a correlation coefficient (R(2)) greater than 0.9 for TFA and PUFA, with a higher correlation assigned to PUFA (r = 0.988; p < 0.001), followed by TFA (r = 0.959; p < 0.01) and MUFA (r = 0.844; p < 0.05). Principal component analysis of the fatty acid (FA) profiles showed discrimination between chamia and both fresh and heated SSO. A high stability of SSO against isomerization reactions as compared to their chamia sample counterpart has been noted. These findings suggest that the food industry engenders relatively higher changes in fatty acid configurations than the frying process.

  11. The use of the CELLection kit in the isolation of carcinoma cells from mononuclear cell suspensions

    DEFF Research Database (Denmark)

    Werther, K; Normark, M; Hansen, B F;

    2000-01-01

    A study was performed to evaluate in vitro the sensitivity, specificity and variability of a new immunomagnetic microbead isolation technique which provides subsequent immunological staining of captured carcinoma cells. In a mixture of peripheral blood mononuclear cells (PBMCs) and human carcinoma...... cells the epithelial cancer cells were isolated with the Dynal((R)) RAM IgG1 CELLection Kit using Dynabeads M-280 coated with a rat monoclonal antibody (Mab) against mouse IgG1. The rat Mab was biotinylated and attached to Dynabeads via streptavidin and a DNA linker. The anti-epithelial monoclonal mouse...... an average recovery of approximately 60% of a human colon carcinoma cell line HCC-2998 seeded in 5.10(6) PBMCs was obtained, and the recovered cells could subsequently be immunologically stained for the surface antigen CD87 (urokinase plasminogen activator receptor). No positive stained cells were found...

  12. Oil persistence on beaches in Prince William Sound - a review of SCAT surveys conducted from 1989 to 2002.

    Science.gov (United States)

    Taylor, Elliott; Reimer, Doug

    2008-03-01

    In 2002, 13 years after the Exxon Valdez oil spill (EVOS), 39 selected sites in Prince William Sound (PWS) were re-surveyed following established shoreline cleanup assessment team (SCAT) field observation procedures to document surface and sub-surface oiling conditions in shoreline sediments and to compare results with those from previous Shoreline Cleanup Assessment Team (SCAT) surveys and other surveys in PWS. The selected sites are locations where EVOS oil persisted in 1992, at the time the Federal and State On-Scene Coordinators determined that the cleanup was complete and that further cleanup activities would provide no net environmental benefit. These sites had been included in a 2001 NOAA survey of shoreline oiling conditions and account for 88% of the sub-surface oil residues (SSO) oil documented by that study. The 2002 field survey found isolated occurrences of residual EVOS surface oil residues (SO) in the form of weathered asphalt pavement at 15 of the 39 sites. This residual SO typically consisted of asphalt in mixed sand/gravel substrate, located within a wave shadow effect created by boulders or bedrock in the upper intertidal to supratidal zone. Residual SO, expressed as a continuous oil cover, was less than 200 m(2) within the approximately 111,120 m(2) surveyed. A total of 1182 pits were dug at locations where SSO residues were present in 1992. Six of the 39 sites and 815 (68%) of the pits contained no residual SSO. Eighty-three percent of pits with SSO residues were found primarily in middle to upper intertidal locations. SSO residues commonly occurred in a discontinuous approximately 3 cm thick band 5-10 cm below the boulder/cobble or pebble/gravel veneer. The SO and SSO occurrences in the 2002 survey closely match the locations where they were found in 1992 and earlier surveys; however, in 2002 residual SSO patches are more discontinuous and thinner than they were in the earlier surveys. These sites are biased toward SSO persistence; those that

  13. Structure and regulatory role of the C-terminal winged helix domain of the archaeal minichromosome maintenance complex

    Science.gov (United States)

    Wiedemann, Christoph; Szambowska, Anna; Häfner, Sabine; Ohlenschläger, Oliver; Gührs, Karl-Heinz; Görlach, Matthias

    2015-01-01

    The minichromosome maintenance complex (MCM) represents the replicative DNA helicase both in eukaryotes and archaea. Here, we describe the solution structure of the C-terminal domains of the archaeal MCMs of Sulfolobus solfataricus (Sso) and Methanothermobacter thermautotrophicus (Mth). Those domains consist of a structurally conserved truncated winged helix (WH) domain lacking the two typical ‘wings’ of canonical WH domains. A less conserved N-terminal extension links this WH module to the MCM AAA+ domain forming the ATPase center. In the Sso MCM this linker contains a short α-helical element. Using Sso MCM mutants, including chimeric constructs containing Mth C-terminal domain elements, we show that the ATPase and helicase activity of the Sso MCM is significantly modulated by the short α-helical linker element and by N-terminal residues of the first α-helix of the truncated WH module. Finally, based on our structural and functional data, we present a docking-derived model of the Sso MCM, which implies an allosteric control of the ATPase center by the C-terminal domain. PMID:25712103

  14. Sequence conversion by single strand oligonucleotide donors via non-homologous end joining in mammalian cells.

    Science.gov (United States)

    Liu, Jia; Majumdar, Alokes; Liu, Jilan; Thompson, Lawrence H; Seidman, Michael M

    2010-07-23

    Double strand breaks (DSBs) can be repaired by homology independent nonhomologous end joining (NHEJ) pathways involving proteins such as Ku70/80, DNAPKcs, Xrcc4/Ligase 4, and the Mre11/Rad50/Nbs1 (MRN) complex. DSBs can also be repaired by homology-dependent pathways (HDR), in which the MRN and CtIP nucleases produce single strand ends that engage homologous sequences either by strand invasion or strand annealing. The entry of ends into HDR pathways underlies protocols for genomic manipulation that combine site-specific DSBs with appropriate informational donors. Most strategies utilize long duplex donors that participate by strand invasion. Work in yeast indicates that single strand oligonucleotide (SSO) donors are also active, over considerable distance, via a single strand annealing pathway. We examined the activity of SSO donors in mammalian cells at DSBs induced either by a restriction nuclease or by a targeted interstrand cross-link. SSO donors were effective immediately adjacent to the break, but activity declined sharply beyond approximately 100 nucleotides. Overexpression of the resection nuclease CtIP increased the frequency of SSO-mediated sequence modulation distal to the break site, but had no effect on the activity of an SSO donor adjacent to the break. Genetic and in vivo competition experiments showed that sequence conversion by SSOs in the immediate vicinity of the break was not by strand invasion or strand annealing pathways. Instead these donors competed for ends that would have otherwise entered NHEJ pathways.

  15. Single Sign-On in Cloud Federation using CloudSim

    Directory of Open Access Journals (Sweden)

    Manoj V. Thomas

    2015-05-01

    Full Text Available Single Sign-On (SSO is an authentication mechanism in which a Cloud Service Consumer (CSC needs to be authenticated only once while accessing vari-ous services from multiple service providers, or when accessing multiple services from the same service provid-er. In the case of Cloud Federation, the consumers can get services from various Cloud Service Providers (CSPs who are members of the federation, and SSO can be used to verify the legitimate users without requiring them to get authenticated with each service provider separately. CloudSim is a popular tool used for simulating various cloud computing scenarios. As of now, the simulator lacks effective user authentication and authorization methods with it. In this paper, we discuss the design and implementation of SSO mechanism in the Cloud Federa-tion scenario using the CloudSim toolkit. We have used the Fully Hashed Menezes-Qu-Vanstone (FHMQV pro-tocol for the key exchange and the Symmetric Key En-cryption technique AES-128 for encrypting the identity tokens. We give the workflow model for the proposed approach of SSO in the Cloud Federation and also, the execution time taken in the simulation for various Single Sign-On scenarios where the number of SSO required varies are also shown.

  16. A new hybrid optimization method inspired from swarm intelligence: Fuzzy adaptive swallow swarm optimization algorithm (FASSO

    Directory of Open Access Journals (Sweden)

    Mehdi Neshat

    2015-11-01

    Full Text Available In this article, the objective was to present effective and optimal strategies aimed at improving the Swallow Swarm Optimization (SSO method. The SSO is one of the best optimization methods based on swarm intelligence which is inspired by the intelligent behaviors of swallows. It has been able to offer a relatively strong method for solving optimization problems. However, despite its many advantages, the SSO suffers from two shortcomings. Firstly, particles movement speed is not controlled satisfactorily during the search due to the lack of an inertia weight. Secondly, the variables of the acceleration coefficient are not able to strike a balance between the local and the global searches because they are not sufficiently flexible in complex environments. Therefore, the SSO algorithm does not provide adequate results when it searches in functions such as the Step or Quadric function. Hence, the fuzzy adaptive Swallow Swarm Optimization (FASSO method was introduced to deal with these problems. Meanwhile, results enjoy high accuracy which are obtained by using an adaptive inertia weight and through combining two fuzzy logic systems to accurately calculate the acceleration coefficients. High speed of convergence, avoidance from falling into local extremum, and high level of error tolerance are the advantages of proposed method. The FASSO was compared with eleven of the best PSO methods and SSO in 18 benchmark functions. Finally, significant results were obtained.

  17. Cost-effectiveness of skin-barrier-enhancing emollients among preterm infants in Bangladesh

    Science.gov (United States)

    LeFevre, Amnesty; Shillcutt, Samuel D; Saha, Samir K; Ahmed, ASM Nawshad Uddin; Ahmed, Saifuddin; Chowdhury, MAK Azad; Law, Paul A; Black, Robert; Santosham, Mathuram

    2010-01-01

    Abstract Objective To evaluate the cost-effectiveness of topical emollients, sunflower seed oil (SSO) and synthetic Aquaphor, versus no treatment, in preventing mortality among hospitalized preterm infants (effective if they fell below a ceiling ratio based on the per capita gross national income of Bangladesh (United States dollars, US$ 470). Findings Aquaphor and SSO were both highly cost-effective relative to control, reducing neonatal mortality by 26% and 32%, respectively. SSO cost US$ 61 per death averted and US$ 2.15 per YLL averted (I$ 6.39, international dollars, per YLL averted). Aquaphor cost US$ 162 per death averted and US$ 5.74 per YLL averted (I$ 17.09 per YLL averted). Results were robust to sensitivity analysis. Aquaphor was cost-effective relative to SSO with 77% certainty: it cost an incremental US$ 26 more per patient treated, but averted 1.25 YLLs (US$ 20.74 per YLL averted). Conclusion Topical therapy with SSO or Aquaphor was highly cost-effective in reducing deaths from infection among the preterm neonates studied. The choice of emollient should be made taking into account budgetary limitations and ease of supply. Further research is warranted on additional locally available emollients, use of emollients in community-based settings and generalizability to other geographic regions. PMID:20428367

  18. STEADY-STATE AND IDLE OPTIMIZA- TION OF INTERNAL COMBUSTION ENGINE CONTROL STRATEGIES FOR HYBRID ELECTRIC VEHICLES

    Institute of Scientific and Technical Information of China (English)

    WANG Feng; MAO Xiaojian; YANG Lin; ZHUO Bin

    2008-01-01

    A novel steady-state optimization (SSO) of internal combustion engine (ICE) strategy is proposed to maximize the efficiency of the overall powertrain for hybrid electric vehicles, in which the ICE efficiency, the efficiencies of the electric motor (EM) and the energy storage device are all explicitly taken into account. In addition, a novel idle optimization of ICE strategy is implemented to obtain the optimal idle operating point of the ICE and corresponding optimal parking generation power of the EM using the view of the novel SSO of ICE strategy. Simulations results show that potential fuel economy improvement is achieved relative to the conventional one which only optimized the ICE efficiency by the novel SSO of ICE strategy, and fuel consumption per voltage increment decreases a lot during the parking charge by the novel idle optimization of ICE strategy.

  19. Resposta à aplicação e recuperação de enxofre em cultivos de casa de vegetação em solos com diferentes teores de argila e matéria orgânica Responses to sulfur application and recuperation in greenhouse crops in soils with different clay and organic matter content

    Directory of Open Access Journals (Sweden)

    Danilo dos Santos Rheinheimer

    2007-04-01

    Full Text Available A dinâmica e a disponibilidade do enxofre têm sido pouco estudadas em relação a outros nutrientes, apesar da sua importância como nutriente à planta. Este estudo teve como objetivo avaliar a resposta de algumas culturas à aplicação de sulfato (S-SO4-2 em solos com diferentes teores de argila e matéria orgânica, bem como avaliar a recuperação de enxofre, através de um balanço deste elemento no solo e na planta. O experimento consistiu de seis cultivos sucessivos (canola, soja, feijoeiro, gergelim, trevo vesiculoso e trigo, em vasos em casa de vegetação, utilizando-se quatro tipos de solos e quatro doses de S-SO4-2, com quatro repetições distribuídas ao acaso. Avaliou-se a produção de matéria seca, o teor e o acúmulo de enxofre absorvido pelas culturas. Amostras de solo foram coletadas na camada de 0-10cm após cada cultivo, para determinação do teor de S-SO4-2 disponível. Dentre as culturas estudadas, somente a canola respondeu à aplicação de S-SO4-2. Para as demais culturas, não houve relação entre os teores de S-SO4-2 da camada de 0-10cm e a produção de matéria seca. Solos com diferentes teores de argila e matéria orgânica tiveram comportamento similar em relação à resposta das culturas à fertilização com enxofre; entretanto, a disponibilidade deste nutriente foi maior nos solos mais argilosos.The sulfur (S dynamic and availability is less studied than others nutrients, even though S is an essential nutrient for crops production. This study was carried out to evaluate the crop responses to S-SO4-2 application in different soils and to study S recuperation by balancing it in soil and plants. The study was composed by a greenhouse experiment with six successive crops (canola, soybean, black bean, sesame, clover and wheat using four soils and four S-SO4-2 levels (0, 5, 10 and 20mg kg-1 to evaluate immediate and residual effect of S-SO4-2 application. Soil samples were taken from 0-10 cm layer before and

  20. Solution accuracies of finite element reentry heat transfer and thermal stress analyses of Space Shuttle Orbiter

    Science.gov (United States)

    Ko, William L.

    1988-01-01

    Accuracies of solutions (structural temperatures and thermal stresses) obtained from different thermal and structural FEMs set up for the Space Shuttle Orbiter (SSO) are compared and discussed. For studying the effect of element size on the solution accuracies of heat-transfer and thermal-stress analyses of the SSO, five SPAR thermal models and five NASTRAN structural models were set up for wing midspan bay 3. The structural temperature distribution over the wing skin (lower and upper) surface of one bay was dome shaped and induced more severe thermal stresses in the chordwise direction than in the spanwise direction. The induced thermal stresses were extremely sensitive to slight variation in structural temperature distributions. Both internal convention and internal radiation were found to have equal effects on the SSO.

  1. SeDiCi: An Authentication Service Taking Advantage of Zero-Knowledge Proofs

    Science.gov (United States)

    Grzonkowski, Sławomir

    Transmission of users' profiles over insecure communication means is a crucial task of today's ecommerce applications. In addition, the users have to createmany profiles and remember many credentials. Thus they retype the same information over and over again. Each time the users type their credentials, they expose them to phishing or eavesdropping attempts.These problems could be solved by using Single Sign-on (SSO). The idea of SSO is that the users keep using the same set of credentials when visiting different websites. For web-aplications, OpenID1. is the most prominent solution that partially impelemtns SSO. However, OpenID is prone to phishing attempts and it does not preserve users' privacy [1].

  2. Application of Open Source Software by the Lunar Mapping and Modeling Project

    Science.gov (United States)

    Ramirez, P.; Goodale, C. E.; Bui, B.; Chang, G.; Kim, R. M.; Law, E.; Malhotra, S.; Rodriguez, L.; Sadaqathullah, S.; Mattmann, C. A.; Crichton, D. J.

    2011-12-01

    The Lunar Mapping and Modeling Project (LMMP), led by the Marshall Space Flight center (MSFC), is responsible for the development of an information system to support lunar exploration, decision analysis, and release of lunar data to the public. The data available through the lunar portal is predominantly derived from present lunar missions (e.g., the Lunar Reconnaissance Orbiter (LRO)) and from historical missions (e.g., Apollo). This project has created a gold source of data, models, and tools for lunar explorers to exercise and incorporate into their activities. At Jet Propulsion Laboratory (JPL), we focused on engineering and building the infrastructure to support cataloging, archiving, accessing, and delivery of lunar data. We decided to use a RESTful service-oriented architecture to enable us to abstract from the underlying technology choices and focus on interfaces to be used internally and externally. This decision allowed us to leverage several open source software components and integrate them by either writing a thin REST service layer or relying on the API they provided; the approach chosen was dependent on the targeted consumer of a given interface. We will discuss our varying experience using open source products; namely Apache OODT, Oracle Berkley DB XML, Apache Solr, and Oracle OpenSSO (now named OpenAM). Apache OODT, developed at NASA's Jet Propulsion Laboratory and recently migrated over to Apache, provided the means for ingestion and cataloguing of products within the infrastructure. Its usage was based upon team experience with the project and past benefit received on other projects internal and external to JPL. Berkeley DB XML, distributed by Oracle for both commercial and open source use, was the storage technology chosen for our metadata. This decision was in part based on our use Federal Geographic Data Committee (FGDC) Metadata, which is expressed in XML, and the desire to keep it in its native form and exploit other technologies built on

  3. EXPRESSION, PURIFICATION, AND SMALL ANGLE X-RAY SCATTERING OF DNA REPLICATION AND REPAIR PROTEINS FROM THE HYPERTHERMOPHILE SULFOLOBUS SOLFATARICUS

    Energy Technology Data Exchange (ETDEWEB)

    Patterson, S.M.; Hatherill, J.R.; Hammel, M.; Hura, G.L.; Tainer, J.A.; Yannone, S.M.

    2008-01-01

    Vital molecular processes such as DNA replication, transcription, translation, and maintenance occur through transient protein interactions. Elucidating the mechanisms by which these protein complexes and interactions function could lead to treatments for diseases related to DNA damage and cell division control. In the recent decades since its introduction as a third domain, Archaea have shown to be simpler models for complicated eukaryotic processes such as DNA replication, repair, transcription, and translation. Sulfolobus solfataricus is one such model organism. A hyperthermophile with an optimal growth temperature of 80°C, Sulfolobus protein-protein complexes and transient protein interactions should be more stable at moderate temperatures, providing a means to isolate and study their structure and function. Here we provide the initial steps towards characterizing three DNA-related Sulfolobus proteins with small angle X-ray scattering (SAXS): Sso0257, a cell division control and origin recognition complex homolog, Sso0768, the small subunit of the replication factor C, and Sso3167, a Mut-T like protein. SAXS analysis was performed at multiple concentrations for both short and long exposure times. The Sso0257 sample was determined to be either a mixture of monomeric and dimeric states or a population of dynamic monomers in various conformational states in solution, consistent with a fl exible winged helix domain. Sso0768 was found to be a complex mixture of multimeric states in solution. Finally, molecular envelope reconstruction from SAXS data for Sso3167 revealed a novel structural component which may function as a disordered to ordered region in the presence of its substrates and/or protein partners.

  4. La ecotoxicidad y el pasto japonesa (Leersia sp.) en la fitorremediación de un Gleysol contaminado con petróleo fresco, intemperizado y sulfatos.

    OpenAIRE

    Arias Trinidad, Alfredo

    2013-01-01

    El estudio de ecotoxicidad y fitorremediación de un Gleysol (GL) contaminado con petróleo fresco (PF) e intemperizado (PI) y sulfatos solubles (S-SO4), se realizo para seleccionar indicadores de toxicidad biológicos, evaluar el potencial del pasto japonesa (PJ) en la descontaminación de estos suelos, la presencia de S-SO4 en su interacción con los hidrocarburos, las bacterias sulfato reductoras y actividad enzimática de la arilsulfatasa (ASA). Dos GLs se usaron para establecer los bioensayos ...

  5. The archaeal “7 kDa DNA-binding” proteins: extended characterization of an old gifted family

    OpenAIRE

    Valentina Kalichuk; Ghislaine Béhar; Axelle Renodon-Cornière; Georgi Danovski; Gonzalo Obal; Jacques Barbet; Barbara Mouratou; Frédéric Pecorari

    2016-01-01

    International audience; The " 7 kDa DNA-binding " family, also known as the Sul7d family, is composed of chromatin proteins from the Sulfolobales archaeal order. Among them, Sac7d and Sso7d have been the focus of several studies with some characterization of their properties. Here, we studied eleven other proteins alongside Sac7d and Sso7d under the same conditions. The dissociation constants of the purified proteins for binding to double-stranded DNA (dsDNA) were determined in phosphate-buff...

  6. Advances of evolutionary computation methods and operators

    CERN Document Server

    Cuevas, Erik; Oliva Navarro, Diego Alberto

    2016-01-01

    The goal of this book is to present advances that discuss alternative Evolutionary Computation (EC) developments and non-conventional operators which have proved to be effective in the solution of several complex problems. The book has been structured so that each chapter can be read independently from the others. The book contains nine chapters with the following themes: 1) Introduction, 2) the Social Spider Optimization (SSO), 3) the States of Matter Search (SMS), 4) the collective animal behavior (CAB) algorithm, 5) the Allostatic Optimization (AO) method, 6) the Locust Search (LS) algorithm, 7) the Adaptive Population with Reduced Evaluations (APRE) method, 8) the multimodal CAB, 9) the constrained SSO method.

  7. Subsynchronous Oscillation Problem Research in the UHVDC System of a Regional Power Grid in China

    Directory of Open Access Journals (Sweden)

    Qu Ying

    2016-01-01

    Full Text Available Along with the grid structure being more and more complex and the rapid development of the HVDC system, studying the subsynchronous oscillation (SSO problem on HVDC system has more engineering practice significance. The paper studies subsynchronous oscillations problem of generators near the ±800kV UHVDC converter station, and analyzes the subsynchronous oscillation possibilities through PSCAD/EMTDC simulation. At last, though the researched UHVDC thermal plants have none SSO risk but it needs other measures to make the relevant generators return on normal operation.

  8. 依托生活之本 丰富德育之源——由“东方神童”的故事看中国德育的现状%Relying on the Principle of Life and Enriching the Source of Moral Education

    Institute of Scientific and Technical Information of China (English)

    俞美亚

    2011-01-01

    Starting off from a story of "oriental child prodigy", this paper analyzes the status quo of Chinese moral education. For a long time, under the influence of utilitarianism, doctrinarism and empiricism, moral education is gradually far away from life. The%本文从“东方神童”的故事引入,对中国的德育现状进行了分析。长期以来,在功利主义、教条主义、经验主义等各种因素的影响下,德育逐渐与生活疏离。在此,笔者结合自己的班级管理经验谈论了如何在生活中开展德育工作,让德育工作生活化。

  9. 依托太行山红色文化推进大学生社会主义核心价值观教育%To promote the education of college students’ socialist core values relying on the Taihang red culture

    Institute of Scientific and Technical Information of China (English)

    苗丽

    2016-01-01

    红色文化是在中国共产党领导下中国人民经过长期浴血奋战中形成的伟大精神产物和物质载体。太行山红色文化囊括了革命先辈浴血前行的功绩,更有燕赵人民不屈不挠、奋勇抵抗的事迹见证。高校要充分发挥红色文化与社会主义核心价值观的育人功能,将红色文化内涵转变为当代大学生的精神气质,从多个方向,多个层次发掘和拓展大学生的红色人文主义精神,从而实现社会主义核心价值观教育的实效性。%Red Culture is the material carrier and spiritual product from CPC leading Chinese people fighting for liberation. The Taihang Red Culture consists of the achievements of the revolutionary martyrs, and the proof of Yan Zhao people fighting bravely against the enemy. Colleges and universities should make use of the cultivating functions of the Red Culture and the socialist core values, converting the Red Culture connotation into the college students’ spirit. It is suggested to explore and expand the red humanist spirit in college students, realizing the effectiveness of education of socialist core values.

  10. Rely on the Library Website Platform for Network Information Advisory Services:——Concurrently Discusses the Homepage Making Principle and the Homepage Maintenance%依靠图书馆网页平台开展网络信息咨询服务——兼谈网页制作原则和网页维护

    Institute of Scientific and Technical Information of China (English)

    周崇洲

    2006-01-01

    图书馆网页是网络信息咨询服务的主要平台,具有非常重要的意义,高校图书馆网页的制作必须遵循一定的原则,利用图书馆网页可以开展多种形式的网络信息咨询服务,注重网页的后期维护,是开展网络信息咨询服务的有效保障.

  11. 坚持民族文化传统依靠自主科技创新推进馒头生产经营产业化%ADHERING TO THE TRADITIONAL CULTURE; RELYING ON INDEPENDENT SCI- TECH INNOVATION;PROMOTING THE INDUSTRALIZATION OF BUNS AS STAPLE FOOD

    Institute of Scientific and Technical Information of China (English)

    苏东民

    2007-01-01

    馒头是由中国人发明、发展的一类发酵面团汽蒸食品,具有鲜明的民族文化特色,但其生产经营尚未完全实现工业化、现代化.中国应该坚定自己的文化选择,坚持本民族的食品文化传统,以馒头作为重要主食发展更为营养、安全、健康的主食体系.发展馒头主食产业必须立足于社会经济发展的需要,通过加强自主科技创新,以适应食品消费特点的研究来引领相关工艺和设备的研究开发工作.

  12. Biomechanical performance of resin composites relying on biomimetic analogs and calcium phosphates for dentin remineralization = : Desempenho biomecânico de compósitos experimentais com análogos biomiméticos e fosfatos de cálcio para a remineralização da dentina

    OpenAIRE

    2015-01-01

    O objetivo do estudo foi gerar uma nova metodologia restauradora, através de um compósito fluído fotopolimerizável com fosfatos de cálcio e incorporação de tri-metafosfato de sódio em primer autocondicionante para auto-produção de fosfoproteína biomineralizadora da matriz de colágeno na interface de união. A hipótese desse trabalho foi de que a inovadora metodologia irá recuperar as propriedades mecânicas, remineralizará e aumentará a longevidade da interface de união. No Capítulo 1 foi reali...

  13. Raciocínio sobre probabilidades condicionais: as evidências a favor da hipótese freqüentista se fundamentam em comparações errôneas Reasoning about conditional probabilities: the evidence for the frequentist hypothesis has relied on flawed comparisons

    Directory of Open Access Journals (Sweden)

    David O'Brien

    2004-04-01

    Full Text Available Gigerenzer e Hoffrage (1995 e Cosmides e Tooby (1996 propuseram uma hipótese freqüentista a qual afirma que, embora as pessoas raramente façam julgamentos sobre probabilidades condicionais que estejam de acordo com padrões normativos do teorema de Bayes, tais julgamentos podem ser feitos mais comumente quando um problema é apresentado em termos de freqüências do que em probabilidades. Esses dois artigos apresentam 10 experimentos com 89 versões de problemas que apóiam sua afirmação, com sujeitos resolvendo, mais freqüentemente, de forma consistente, problemas no formato freqüentista do que no formato probabilista. Os resultados de dois experimentos relatados no presente estudo mostram, contudo, que os achados destes autores podem ser explicados como resultantes de dois problemas metodológicos que têm relação com a presença versus ausência de um formato de resposta e com o uso de números inteiros versus frações decimais. No Experimento 1, encontrou-se que os problemas freqüentistas eram resolvidos apenas quando apresentados com um formato de resposta que pode estimular suposições precisas e, no Experimento 2, que problemas de formato freqüentista e probabilista eram resolvidos na mesma freqüência quando apresentados com formato de resposta e com números inteiros. Os resultados são discutidos em termos das suas implicações contra a hipótese freqüentista.Gigerenzer and Hoffrage (1995 and Cosmides and Tooby (1996 proposed a frequentist hypothesis that claims that although people rarely make judgements about conditional probabilities that concord with the normative standards of Bayes's theorem, such judgements can be elicited when a problem is presented in terms of frequencies rather than probabilities. These two articles together reported 10 experiments with 89 problem versions in support of their prediction, with people consistently solving frequentist-formatted problems more frequently than probabilist-formatted problems. The results of two experiments reported here, however, show that their results can be explained as resulting from two experimental confounds that have to do with the presence vs. absence of a response format and with the use of whole numbers vs. decimal fractions. In Experiment 1 we found that the frequentist problems were solved only when presented with a response format that can encourage accurate guesses, and in Experiment 2 we found that frequentist- and probabilist- formatted problems were solved equally often when presented with the response format and with whole numbers rather than with decimal fractions. The results are discussed in terms of their negative implications for the frequentist hypothesis.

  14. Research on the Reform of the “Wine Knowledge and Mixing Technology” Curriculum Relying on Industry Jobs%依托行业岗位的《酒水知识与调酒技术》课程改革研究

    Institute of Scientific and Technical Information of China (English)

    钟真

    2016-01-01

    The content of higher vocational education curriculum should take the“position”as the mainline,take“employment”as the guide and take “work”as the core.This paper puts forward the reform of teaching content、teaching methods and means of examination in “Wine knowledge and technology”curriculum,according to the specific needs of corresponding position in the market.%高职教育课程内容应该以“岗位”为主线,以“就业”为导向,以“工作任务”为核心,文章依据市场酒水岗位的具体需求,提出《酒水知识与调酒技术》课程的教学内容改革、教学方法改革和考核手段改革。

  15. Cultivating Practical Food Talents Relying on Region Industry Economy---Taking the “Topic Learning Method ”as an Example%依托区域产业经济,培养应用型食品人才--以“课题学习法”为例

    Institute of Scientific and Technical Information of China (English)

    邢淑婕; 刘开华

    2015-01-01

    Investigating and analyzing the status of talent demand of Henan Province food business ,discussing the application of “topic learning method ” in characteristic talent and innovative talents cultivation of food specialty and combining the characteristic and talent training of food specialty of Xinyang College of Agriculture and Forestry , all this provides a new way of how to train practical food engi-neering talents .%调查分析了河南省食品企业人才需求现状,结合信阳农林学院食品专业特点与人才培养的现状,探讨了“课题学习法”在食品专业特色人才、创新人才培养中的应用,为应用型食品工程人才的培养提供了新途径。

  16. 提高顾客忠诚依赖目标产品还是支持性服务--顾客满意度与顾客忠诚度元分析研究%Dose It Rely on Target Product or Supportive Service to Improve Customer Loyalty---A Meta-Analysis on Customer satisfaction and Customer Loyalty

    Institute of Scientific and Technical Information of China (English)

    林业进; 张红娜

    2014-01-01

    It is a great marketing target of enterprise to cultivate the customer loyalty through improving customer satisfaction.Under the process of improving customer loyalty,different customer satisfaction aspect makes different sense.In this study,the meta-analysis is done to research the relationship between customer satisfaction and customer loyalty with 141 empirical researches which contain 21 Chinese researches and 120 foreign references and the total sample size is 72670.In this study,it draws the conclusion is that the first it is positive relationship between customer satisfaction and customer loyalty and the second is different customer satisfaction aspect has different influence to customer loyalty;target product satisfaction has deeper degree of influence than supportive service satisfaction to customer loyalty and the third is attitude loyalty is more sensitive with supportive service satisfaction ,and behavior loyalty is more sensitive with target product satisfaction.%通过提高顾客满意度从而培养忠诚客户一直是企业的营销目标。通过对国内外核心期刊的141篇(英文120篇,中文为21篇)实证研究进行元分析,总样本量为72670。得出结论为:(1)顾客满意度与顾客忠诚度正相关;(2)目标产品满意度对顾客忠诚度的影响程度大于支持性服务满意度对顾客忠诚度的影响;(3)态度忠诚对支持性服务满意度更为敏感,行为忠诚对目标产品满意度更为敏感。

  17. 依托行业·注重实训·提升技能——以‘镶行社计调业务——组团计调”为例%Relying on Industry, Focus on Training, Upgrade the Skills Take "The Travel Agency Adjustment Business-group Adjustment" as an example

    Institute of Scientific and Technical Information of China (English)

    赵丽娅

    2012-01-01

    With the development of tourism, travel agents require more tour guides service personnel, especially the talent to master the special skills and practical skills. Actual adjustment for the work process as the main line, to organize course content of the "travel agency adjustment services" to the logical order to complete the actual work tasks, career job require- ments and the needs of the students' development, design-level tourism adjustment for the business curriculum, students have engaged in the work of adjustment for the quality and the formation of the corresponding professional competence.%随着旅游业的发展,旅行社对导游服务人才的需求也越来越大,特别是掌握专业技能和实践技能的人才。本文以实际计调工作过程为主线,以完成实际工作任务的逻辑顺序来对旅行社计调业务课程的教学内容进行组织,从企业的职业岗位要求和学生发展的需求出发,设计中职旅游计调业务的课程体系,使学生具备从事计调工作的素质并形成相应的职业能力。

  18. Engineering expression of the heavy metal transporter MerC in Saccharomyces cerevisiae for increased cadmium accumulation

    Energy Technology Data Exchange (ETDEWEB)

    Kiyono, Masako; Miyahara, Kiyomi; Sone, Yuka; Nakamura, Ryosuke; Sakabe, Kou [Kitasato Univ., Tokyo (Japan). Dept. of Public Health and Molecular Toxicology; Pan-Hou, Hidemitsu [Setsunan Univ., Osaka (Japan). Faculty of Pharmaceutical Sciences; Uraguchi, Shimpei [Tokyo Univ. (Japan). Biotechnology Research Center

    2010-03-15

    The merC gene from the Tn21-encoded mer operon has potential uses as a molecular tool for bioremediation. It was overexpressed as the fusion proteins MerC-Sso1p or MerC-Vam3p in Saccharomyces cerevisiae. Green fluorescent protein (GFP)-MerC-Sso1p fusion proteins located primarily in the plasma membrane, although some protein was detected in the endoplasmic reticulum. In contrast, GFP-MerC-Vam3p was expressed in the vacuolar membranes. These results suggest that yeast Sso1p and Vam3p are essential for targeting molecules to the plasma and vacuolar membranes, respectively. Significantly more cadmium ions were accumulated by yeast cells expressing MerC-Sso1p than with MerC-Vam3p or control cells. These results suggest that expression of MerC in the plasma membrane may be a particularly promising strategy for improving accumulation of cadmium in yeast. (orig.)

  19. Transcriptome profiles of hybrid poplar (Populus trichocarpa × deltoides) reveal rapid changes in undamaged, systemic sink leaves after simulated feeding by forest tent caterpillar (Malacosoma disstria).

    Science.gov (United States)

    Philippe, Ryan N; Ralph, Steven G; Mansfield, Shawn D; Bohlmann, Jörg

    2010-11-01

    • Poplar has been established as a model tree system for genomic research of the response to biotic stresses. This study describes a series of induced transcriptome changes and the associated physiological characterization of local and systemic responses in hybrid poplar (Populus trichocarpa × deltoides) after simulated herbivory. • Responses were measured in local source (LSo), systemic source (SSo), and systemic sink (SSi) leaves following application of forest tent caterpillar (Malacosoma disstria) oral secretions to mechanically wounded leaves. • Transcriptome analyses identified spatially and temporally dynamic, distinct patterns of local and systemic gene expression in LSo, SSo and SSi leaves. Galactinol synthase was strongly and rapidly upregulated in SSi leaves. Genome analyses and full-length cDNA cloning established an inventory of poplar galactinol synthases. Induced changes of galactinol and raffinose oligosaccharides were detected by anion-exchange high-pressure liquid chromatography. • The LSo leaves showed a rapid and strong transcriptome response compared with a weaker and slower response in adjacent SSo leaves. Surprisingly, the transcriptome response in distant, juvenile SSi leaves was faster and stronger than that observed in SSo leaves. Systemic transcriptome changes of SSi leaves have signatures of rapid change of metabolism and signaling, followed by later induction of defense genes.

  20. Estimation of low-temperature spectra behavior in Nd-doped Sc2SiO5 single crystal.

    Science.gov (United States)

    Zheng, Lihe; Xu, Jun; Su, Liangbi; Li, Hongjun; Wang, Qingguo; Ryba-Romanowski, Witold; Lisiecki, Radosław; Wu, Feng

    2009-11-15

    High optical quality Nd-doped Sc(2)SiO(5) crystal with size of diameter 27 mm x 60 mm was obtained by Czochralski method. An x-ray diffraction pattern of Nd:SSO crystal confirmed that the as-grown crystal was isostructual with the SSO crystal. Absorption and emission spectra were recorded at 10 K. The Judd-Ofelt theory was applied to obtain standard parameters Omega t(t=2,4,6) and the fitting result of experimental absorption line strengths, which provided the F3/24 radiative lifetime (tau(rad)) of 219 micros and the luminescence branching ratio beta of 0.57 for the (4)F(3/2)-(4)I(9/2) laser transition. The stimulated emission cross section of 1.04 x 10(-19) cm(2) at 1074 nm was calculated using the Füchtbauer-Ladenburg equation. The (4)F(3/2) luminescence lifetimes with 215 micros at 10 K and 198 micros at 300 K were determined from luminescence decay curves, indicating high quantum efficiency in the Nd:SSO crystal. All these results showed that Nd:SSO would be a promising gain media in solid-state lasers.

  1. CERN Summer Student Report

    CERN Document Server

    Storo Nyflott, Martin

    2016-01-01

    This report discusses several projects, involving integration of GitHub and Jenkins through SSO, standardization of CMake find-modules, automation of lcgcmake dependency updates, implementation of Jenkins email-templates, and setting up compilation of Fedora using Docker on Travis. Each project is discussed individually, while the time-phase among each project may overlap each other.

  2. Predicting Visibility of Aircraft

    Science.gov (United States)

    Watson, Andrew; Ramirez, Cesar V.; Salud, Ellen

    2009-01-01

    Visual detection of aircraft by human observers is an important element of aviation safety. To assess and ensure safety, it would be useful to be able to be able to predict the visibility, to a human observer, of an aircraft of specified size, shape, distance, and coloration. Examples include assuring safe separation among aircraft and between aircraft and unmanned vehicles, design of airport control towers, and efforts to enhance or suppress the visibility of military and rescue vehicles. We have recently developed a simple metric of pattern visibility, the Spatial Standard Observer (SSO). In this report we examine whether the SSO can predict visibility of simulated aircraft images. We constructed a set of aircraft images from three-dimensional computer graphic models, and measured the luminance contrast threshold for each image from three human observers. The data were well predicted by the SSO. Finally, we show how to use the SSO to predict visibility range for aircraft of arbitrary size, shape, distance, and coloration. PMID:19462007

  3. The Use of Chaff in Space as a Jamming Device between Ground Stations and Satellites

    Science.gov (United States)

    1988-12-01

    of Fi•Yu es Figure Page 1. Rasic Orbital Parameters . . . . . . . .... 2. Satellite Orientation for Cases I, II, III, and IV...Moti r on Spin Direction PCe of sot on c.SSO..!Path of notion Case.X AngLe of Tk~t Case-III Cast IV Figure 2. SatelLite Orientation for Cases I, II

  4. De Sepulcro Laurentii Vallae quid veri habeat : Tracing the Tomb Monument of Lorenzo Valla in St. John Lateran, Rome

    NARCIS (Netherlands)

    de Jong, Johannes

    2014-01-01

    Zusammenfassung: In diesem Artikel wird die Geschichte des Grabmals von Lorenzo Valla (ca. 1407–1457) nachgezeichnet: Der Sarkophag und die Skulptur des liegenden Mannes in der Cappella del Crocifi sso der Kirche San Giovanni in Laterano (Rom) werden im Allgemeinen als das Grabmal Vallas angesehen.

  5. Analysis of received AIS data from a LEO Cubesat

    DEFF Research Database (Denmark)

    Larsen, Jesper Abildgaard; Mortensen, Hans Peter; Tausen, Mathias

    2014-01-01

    The AAUSAT3 satellite is a 1U cubesat, which has been developed by students at Aalborg Uni- versity, Denmark in collaboration with the Danish Maritime Authority. The satellite was launched into a polar DD-SSO orbit of 800 km altitude on February 25th 2013 on a mission to monitor ships from space...

  6. Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.

    Directory of Open Access Journals (Sweden)

    Birgit Märtens

    Full Text Available Recent studies identified a 5´ to 3´ exoribonuclease termed Sso-RNase J in the crenarchaeon Sulfolobus solfataricus (Sso, which has been reclassified to the aCPSF2 (archaeal cleavage and polyadenylation specificity factor 2 group of β-CASP proteins. In this study, the Sso-aCPSF2 orthologue of Sulfolobus acidocaldarius (Saci-aCPSF2 was functionally characterized. Like Sso-aCPSF2, Saci-aCPSF2 degrades RNA with 5´ to 3´ directionality in vitro. To address the biological significance of Saci-aCPSF2, a deletion mutant was constructed, and the influence of Saci-aCPSF2 on the transcriptome profile was assessed employing high throughput RNA sequencing. This analysis revealed 560 genes with differential transcript abundance, suggesting a considerable role of this enzyme in RNA metabolism. In addition, bioinformatic analyses revealed several transcripts that are preferentially degraded at the 5´ end. This was exemplarily verified for two transcripts by Northern-blot analyses, showing for the first time that aCPSF2 proteins play a role in 5' to 3' directional mRNA decay in the crenarchaeal clade of Archaea.

  7. Tweet for the cure: A snapshot of Twitter usage by 3 U.S. oncologic professional societies

    Directory of Open Access Journals (Sweden)

    Sachin R. Jhawar, MD

    2017-07-01

    Conclusion: ASTRO's use of Twitter lags behind ASCO and SSO. Although all 3 societies show increased Twitter use during their annual meetings, they should work toward more meaningful engagement throughout the year. The new metrics of tweet density and supporter ratio will serve as benchmarks for member engagement in future studies.

  8. CHARACTERIZATION OF SINGLE-STRAND ORIGINS OF CRYPTIC ROLLING-CIRCLE PLASMIDS FROM BACILLUS-SUBTILIS

    NARCIS (Netherlands)

    MEIJER, WJJ; VENEMA, G; BRON, S

    1995-01-01

    In this paper we describe the isolation and characterization of single strand origins (SSOs) of several cryptic Bacillus subtilis plasmids which use the rolling-circle mechanism of replication, The plasmids used in this study involved pTA1015, pTA1020, pTA1030, pTA1040, pTA1050 and pTA1060, The SSO

  9. Review on Countermeasures for Sub-Synchronous Oscillations in Power Systems%电力系统次同步振荡抑制方法概述

    Institute of Scientific and Technical Information of China (English)

    王瑞闯

    2011-01-01

    The paper presents four types of Subsynchronous Oscillation (SSO), including the inductive generator effect,torsion oscillation. transient torque amplification and SSO caused by other electrical equipment. The paper describes the basic concept and generation mechanism of the SSO. reviews the restraining methods used to counter the SSO and the current development of each method. and finally points out the issues for further studies.%介绍了次同步振荡研究的感应发电机效应、扭转相互作用、暂态力矩放大以及由其他电气装置引起次同步振荡的主要内容.阐述了次同步振荡的基本概念及产生的机理,并介绍了目前抑制次同步振荡的方法及发展现状,指出了需进一步研究的问题.

  10. De Sepulcro Laurentii Vallae quid veri habeat : Tracing the Tomb Monument of Lorenzo Valla in St. John Lateran, Rome

    NARCIS (Netherlands)

    de Jong, Johannes

    2014-01-01

    Zusammenfassung: In diesem Artikel wird die Geschichte des Grabmals von Lorenzo Valla (ca. 1407–1457) nachgezeichnet: Der Sarkophag und die Skulptur des liegenden Mannes in der Cappella del Crocifi sso der Kirche San Giovanni in Laterano (Rom) werden im Allgemeinen als das Grabmal Vallas angesehen.

  11. Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.

    Science.gov (United States)

    Märtens, Birgit; Amman, Fabian; Manoharadas, Salim; Zeichen, Lukas; Orell, Alvaro; Albers, Sonja-Verena; Hofacker, Ivo; Bläsi, Udo

    2013-01-01

    Recent studies identified a 5´ to 3´ exoribonuclease termed Sso-RNase J in the crenarchaeon Sulfolobus solfataricus (Sso), which has been reclassified to the aCPSF2 (archaeal cleavage and polyadenylation specificity factor 2) group of β-CASP proteins. In this study, the Sso-aCPSF2 orthologue of Sulfolobus acidocaldarius (Saci-aCPSF2) was functionally characterized. Like Sso-aCPSF2, Saci-aCPSF2 degrades RNA with 5´ to 3´ directionality in vitro. To address the biological significance of Saci-aCPSF2, a deletion mutant was constructed, and the influence of Saci-aCPSF2 on the transcriptome profile was assessed employing high throughput RNA sequencing. This analysis revealed 560 genes with differential transcript abundance, suggesting a considerable role of this enzyme in RNA metabolism. In addition, bioinformatic analyses revealed several transcripts that are preferentially degraded at the 5´ end. This was exemplarily verified for two transcripts by Northern-blot analyses, showing for the first time that aCPSF2 proteins play a role in 5' to 3' directional mRNA decay in the crenarchaeal clade of Archaea.

  12. The implementation and investigation of securing web applications upon multi-platform for a single sign-on functionality

    Directory of Open Access Journals (Sweden)

    Hsien-Yu Lee

    2016-03-01

    Full Text Available Nowadays, the world is moving toward mobilized communities as the development of internet and web applications. Almost all of the daily activities and transactions can be done through the internet. Web applications become trends as e-business and e-commerce systems continue to make our lives easier and better without our noticing. These web applications were developed from various frameworks, programming languages and platforms etc. over the internet. Computer technology is a time saving and quality improving revolution. When the enterprise will build up the vision of computer digitalization, i.e. hosting e-Enterprise engineering, cloud platform or information systems integration, etc. Under the web world, the single sign-on (SSO concept was invented as problem-solving method about one login as you go authorized systems. In other words, web securities are confronting some challenges under the networked era of the emergency web. Therefore the SSO functionality based on multiple platforms and web enabling technologies is put forward and it also secures web applications developed from diverse programming. The proposed method of secure login utility is created as an obvious solution to the general functionality of SSO. Hereby, we also will reveal two application instances of the secure login utility to implement the idea of SSO realized in the enterprise systems.

  13. Compact intra-cavity pumped low-threshold passively Q-switched Ho:Sc2SiO5 laser by a LD-pumped Tm:YAP laser at room temperature

    Science.gov (United States)

    Yang, Xiao-tao; Xie, Wen-qiang; Liu, Long; Li, Lin-jun

    2017-08-01

    A compact intra-cavity pumped low-threshold passively Q-switched (PQS) Ho:Sc2SiO5 (Ho:SSO) laser is reported for the first time. The Tm:YAlO3 (Tm:YAP) crystal and the Ho:SSO crystal are placed in the same laser cavity. A laser diode with a central wavelength of 793 nm is used to realize the output of the Ho:SSO laser. Both the continuous wave (CW) and PQS operation are investigated. A Cr2+:ZnSe is used as the saturable absorber in the PQS Ho:SSO laser. For the CW mode, the laser threshold is only 750 mW, which is 980 mW in the PQS mode. A maximum pulse energy of 699 µJ is primarily obtained, corresponding to the pulse width of 96 ns. The maximum repetition frequency is 1.46 kHz. The maximum pulse peak power can be calculated to be 7.28 kW. The beam quality factor M 2 is calculated to be 1.4 with the maximum output power.

  14. APPROACH TO "FURTHER-REASONABLE-PROGRESS" TO ATTAIN WATER QUALITY STANDARDS

    Science.gov (United States)

    Watershed managers in urban areas in the US are pursuing optimum strategies or plans for managing wastewater treatment plants, stormwater systems, sanitary sewer overflows (SSO) and combined sewer overflows (CSO). Often these strategies are based on an iterative process or a "fur...

  15. 78 FR 28014 - FTA Supplemental Fiscal Year 2013 Apportionments, Allocations, and Program Information

    Science.gov (United States)

    2013-05-13

    ... Readiness for SSO Grant Application and Certification Process 4. Certification of State Safety Oversight... 3A, ``Section 5307 Operating Assistance Special Rule Operator Caps,'' for the systems in large urbanized areas. The calculation of the operating assistance caps in Table 3-A has been updated to...

  16. The assessment and management of older cancer patients : A SIOG surgical task force survey on surgeons' attitudes

    NARCIS (Netherlands)

    Ghignone, F; van Leeuwen, B. L.; Montroni, I; Huisman, M G; Somasundar, P; Cheung, K L; Audisio, R A; Ugolini, G

    Aim: The Surgical Task Force at SIOG (International Society of Geriatric Oncology) designed this survey to explore the surgical oncologists' approach toward elderly cancer patients. Methods: A web-based survey was sent to all members of ESSO (European Society of Surgical Oncology) and SSO (Society

  17. Lessons learned after one year in space for the AAUSAT3 satellite

    DEFF Research Database (Denmark)

    Larsen, Jesper Abildgaard; Mortensen, Hans Peter; Jessen, Troels

    2014-01-01

    The AAUSAT3 satellite is a 1U cubesat, which has been developed by students at Aalborg University, Denmark in collaboration with the Danish Maritime Authority. The satellite was launched into a polar DD-SSO orbit of 800 km altitude on February 25th 2013 on a mission to monitor ships from space...

  18. 78 FR 46005 - NPDES Electronic Reporting Rule

    Science.gov (United States)

    2013-07-30

    ....oeca@epa.gov , Attention Docket ID No. EPA- HQ-OECA-2009-0274. Mail: Send the original and three copies... not limited to any pipe, ditch, channel, tunnel, conduit, well, discrete fissure, container, rolling... Significant Noncompliance (according to EPA policy and guidance) SSL Secure Socket Layer SSO Sanitary Sewer...

  19. Software Simulates Sight: Flat Panel Mura Detection

    Science.gov (United States)

    2008-01-01

    In the increasingly sophisticated world of high-definition flat screen monitors and television screens, image clarity and the elimination of distortion are paramount concerns. As the devices that reproduce images become more and more sophisticated, so do the technologies that verify their accuracy. By simulating the manner in which a human eye perceives and interprets a visual stimulus, NASA scientists have found ways to automatically and accurately test new monitors and displays. The Spatial Standard Observer (SSO) software metric, developed by Dr. Andrew B. Watson at Ames Research Center, measures visibility and defects in screens, displays, and interfaces. In the design of such a software tool, a central challenge is determining which aspects of visual function to include while accuracy and generality are important, relative simplicity of the software module is also a key virtue. Based on data collected in ModelFest, a large cooperative multi-lab project hosted by the Optical Society of America, the SSO simulates a simplified model of human spatial vision, operating on a pair of images that are viewed at a specific viewing distance with pixels having a known relation to luminance. The SSO measures the visibility of foveal spatial patterns, or the discriminability of two patterns, by incorporating only a few essential components of vision. These components include local contrast transformation, a contrast sensitivity function, local masking, and local pooling. By this construction, the SSO provides output in units of "just noticeable differences" (JND) a unit of measure based on the assumed smallest difference of sensory input detectable by a human being. Herein is the truly amazing ability of the SSO, while conventional methods can manipulate images, the SSO models human perception. This set of equations actually defines a mathematical way of working with an image that accurately reflects the way in which the human eye and mind behold a stimulus. The SSO is

  20. Tensions of network security and collaborative work practice: understanding a single sign-on deployment in a regional hospital.

    Science.gov (United States)

    Heckle, Rosa R; Lutters, Wayne G

    2011-08-01

    Healthcare providers and their IT staff, working in an effort to balance appropriate accessibility with stricter security mandates, are considering the use of a single network sign-on approach for authentication and password management. Single sign-on (SSO) promises to improve usability of authentication for multiple-system users, increase compliance, and help curb system maintenance costs. However, complexities are introduced when SSO is placed within a collaborative environment. These complexities include unanticipated workflow implications that introduce greater security vulnerability for the individual user. OBJECTIVES AND METHODOLOGY: In this work, we examine the challenges of implementing a single sign-on authentication technology in a hospital environment. The aim of the study was to document the factors that affected SSO adoption within the context of use. The ultimate goal is to better inform the design of usable authentication systems within collaborative healthcare work sites. The primary data collection techniques used are ethnographically informed - observation, contextual interviews, and document review. The study included a cross-section of individuals from various departments and varying rolls. These participants were a mix of both clinical and administrative staff, as well as the Information Technology group. The field work revealed fundamental mis-matches between the technology and routine work practices that will significantly impact its effective adoption. While single sign-on was effective in the administrative offices, SSO was not a good fit for collaborative areas. The collaborative needs of the clinical staff unearthed tensions in its implementation. An analysis of the findings revealed that the workflow, activities, and physical environment of the clinical areas create increased security vulnerabilities for the individual user. The clinical users were cognizant of these vulnerabilities and this created resistance to the implementation due

  1. OPeNDAP servers like Hyrax and TDS can easily support common single-sign-on authentication protocols using the Apache httpd and related software; adding support for these protocols to clients can be more challenging

    Science.gov (United States)

    Gallagher, J. H. R.; Potter, N.; Evans, B. J. K.

    2016-12-01

    OPeNDAP, in conjunction with the Australian National University, documented the installation process needed to add authentication to OPeNDAP-enabled data servers (Hyrax, TDS, etc.) and examined 13 OPeNDAP clients to determine how best to add authentication using LDAP, Shibboleth and OAuth2 (we used NASA's URS). We settled on a server configuration (architecture) that uses the Apache web server and a collection of open-source modules to perform the authentication and authorization actions. This is not the only way to accomplish those goals, but using Apache represents a good balance between functionality, leveraging existing work that has been well vetted and includes support for a wide variety of web services, include those that depend on a servlet engine such as tomcat (which both Hyrax and TDS do). Or work shows how LDAP, OAuth2 and Shibboleth can all be accommodated using this readily available software stack. Also important is that the Apache software is very widely used and is fairly robust - extremely important for security software components. In order to make use of a server requiring authentication, clients must support the authentication process. Because HTTP has included authentication for well over a decade, and because HTTP/HTTPS can be used by simply linking programs with a library, both the LDAP and OAuth2/URS authentication schemes have almost universal support within the OPeNDAP client base. The clients, i.e. the HTTP client libraries they employ, understand how to submit the credentials to the correct server when confronted by an HTTP/S Unauthorized (401) response. Interestingly OAuth2 can achieve it's SSO objectives while relying entirely on normative HTTP transport. All 13 of the clients examined worked.The situation with Shibboleth is different. While Shibboleth does use HTTP, it also requires the client to either scrape a web page or support the SAML2.0 ECP profile, which, for programmatic clients, means using SOAP messages. Since working with

  2. Extended HLA haplotypes in a Carib Amerindian population: the Yucpa of the Perija Range.

    Science.gov (United States)

    Layrisse, Z; Guedez, Y; Domínguez, E; Paz, N; Montagnani, S; Matos, M; Herrera, F; Ogando, V; Balbas, O; Rodríguez-Larralde, A

    2001-09-01

    Eleven MHC loci haplotypes have been defined among a Carib speaking Amerindian population; the Yucpa, inhabiting the northern section of the Perija Range, on the limits between Colombia and Venezuela. This tribe has been known with the name of "Motilones mansos" and is located close to the Chibcha-Paeze speaking Bari or "Motilones bravos." Seventy-three full blooded Yucpa living at the villages of Aroy, Marewa, and Peraya, were selected using a genealogy previously collected by an anthropologist and tested for Bf-C4AB complement allotypes and by serology, high resolution PCR-SSO and SBT typing for HLA class 1 and class 2 alleles. Combinations of 6 HLA-A, 6 HLA-B, 5 HLA-C, 1 Bf, 3 C4AB, 3 DQA1, 3DQB1 and 2 DPA1 and 2 DPB1 alleles present in this population originate 17 different haplotypes, 3 of which represent 63% of the haplotypic constitution of the tribe. The presence of 13 individuals homozygous for 11-loci haplotypes corroborates the existence of the following allelic combinations: DRB1*0411 DQA1*03011 DQB1*0302 DPA1*01 DPB1*0402 with HLA-A*6801 C*0702 B*3909 BfS C4 32 (f = 0.3372) or with A*0204 C*0702 B*3905 (f = 0.1977) and a third haplotype which differs only in DRB1*0403 and A*2402 (f = 0.0930). The results demonstrate the isolation of the tribe and the existence of high frequencies of a reduced number of "Amerindian" ancestral and novel class 1 and class 2 alleles (B*1522, DRB1*0807) with significant linkage disequilibria. These results will be useful to test the hypothesis that differentiation of Amerindian tribal groups will have to rely on haplotypes and micropolymorphism rather than allelic lineage frequencies due to the uniformity shown thus far by the putative descendants of the original Paleo-Indians.

  3. Grafted vertebral fracture after implant removal in a patient with spine-shortening vertebral osteotomy.

    Science.gov (United States)

    Nakashima, Hiroaki; Yukawa, Yasutsugu; Ito, Keigo; Machino, Masaaki; Kanbara, Shunsuke; Morita, Daigo; Imagama, Shiro; Ishiguro, Naoki; Kato, Fumihiko

    2015-02-01

    We experienced the rare complication of a vertebral fracture that was caused by implant removal after bony fusion had been achieved in a patient who underwent spine-shortening osteotomy (SSO) for tethered cord syndrome (TCS). We propose that the removal of the implant used for SSO should be contraindicated. The patient (a 27-year-old female) presented to our institution with a history of progressive severe lower back pain, gait disturbance, and urinary incontinence. As an infant, she had undergone surgery for spina bifida with lipoma. Magnetic resonance imaging of the spine revealed tethering of the spinal cord to a lipoma. We performed SSO at the level of the L1 vertebra level. After spine shortening and fixation using a posterior approach, the L1 vertebral body was completely removed anteriorly and replaced with a left iliac bone graft. The patient's symptoms improved after surgery. After bony fusion was achieved after surgery, we decided to remove the spinal implant after we explained the advantages and disadvantages of the procedure to the patient. We performed implant removal surgery safely 2 years later; however, the patient complained of severe lower back pain 10 days after the surgery without any history of trauma. Reconstruction computed tomography showed fracture of the grafted vertebra. We performed a repeat posterior fixation, which relieved the lower back pain; she has experienced no complications in the subsequent 5 years. In summary, we report a case of a rare complication of the fracture of a grafted vertebra after removal of an implant used in SSO for TCS. Spinal stability could not be maintained without the spinal posterior implant after SSO. Postoperative fracture after spinal implant removal is rare but possible, and patients must be informed of this potential risk.

  4. Targeted Mutagenesis and Combinatorial Library Screening Enables Control of Protein Orientation on Surfaces and Increased Activity of Adsorbed Proteins.

    Science.gov (United States)

    Cruz-Teran, Carlos A; Carlin, Kevin B; Efimenko, Kirill; Genzer, Jan; Rao, Balaji M

    2016-08-30

    While nonspecific adsorption is widely used for immobilizing proteins on solid surfaces, the random nature of protein adsorption may reduce the activity of immobilized proteins due to occlusion of the active site. We hypothesized that the orientation a protein assumes on a given surface can be controlled by systematically introducing mutations into a region distant from its active site, thereby retaining activity of the immobilized protein. To test this hypothesis, we generated a combinatorial protein library by randomizing six targeted residues in a binding protein derived from highly stable, nonimmunoglobulin Sso7d scaffold; mutations were targeted in a region that is distant from the binding site. This library was screened to isolate binders that retain binding to its cognate target (chicken immunoglobulin Y, cIgY) as well as exhibit adsorption on unmodified silica at pH 7.4 and high ionic strength conditions. A single mutant, Sso7d-2B5, was selected for further characterization. Sso7d-2B5 retained binding to cIgY with an apparent dissociation constant similar to that of the parent protein; both mutant and parent proteins saturated the surface of silica with similar densities. Strikingly, however, silica beads coated with Sso7d-2B5 could achieve up to 7-fold higher capture of cIgY than beads coated with the parent protein. These results strongly suggest that mutations introduced in Sso7d-2B5 alter its orientation relative to the parent protein, when adsorbed on silica surfaces. Our approach also provides a generalizable strategy for introducing mutations in proteins so as to improve their activity upon immobilization, and has direct relevance to development of protein-based biosensors and biocatalysts.

  5. Возможности сфинктеросохраняющего лечения больных местно-распространенным первично-неоперабельным раком прямой кишки

    Directory of Open Access Journals (Sweden)

    Yu. A. Barsukov

    2012-01-01

    Full Text Available The aim of this study was to compare results of sphincter-sparing operations (SSO and abdominoperineal resections (APR in patients  undergoing combined treatment for unresectable locally-advanced rectal cancer.Methods. During September 2007 – January 2011 59 patients were enrolled. Original treatment scheme (RF patent № 2414936 was developed including radiotherapy 40 Gy in 4 Gy fractions, capecitabine 650 mg/m2 bid per os days 1-22, oxaliplatin 50 mg/m2 iv days 3, 10, 17, local hyperthermia on days 8, 12, 15, 17, 2 applications of metronidazole 10 g/m2 per rectum in a polymeric composition. Surgery was carried out following 6–8 weeks. SSO were carried out in 36 patients, APR in 23 patients. Study endpoints included 2-year OS and DFS, local recurrence and distant metastases rate, postoperative complications rate.Results. No significant differences in survival were observed: 2-year OS was 93.2 and 85.6 % (log- rank test p = 0.157 for SSO and APR groups accordingly, 2-year DFS was 88 and 71.9 % (log-rank test p = 0.064. Four (11.1 % patients in SSO group and 4 (17.4 % patients in APR group (р = 0.5511 developed local recurrences, 4 (11.1 % and 7 (30.4 % (р = 0.1293 developed distant metastases. Postoperative complications rate was 27.8 % (n = 10 and 39.1 % (n = 9 (р = 0.5181 in SSO and APR groups accordingly.Conclusions. Sphincter-sparting surgery is justified for unresectable locally advanced rectal cancer when technically feasible.  

  6. Comparative Ethanol Productivities of Two Different Recombinant Fermenting Strains on Source-Separated Organic Waste

    Directory of Open Access Journals (Sweden)

    Valeriy Bekmuradov

    2014-10-01

    Full Text Available Production of biofuel such as ethanol from lignocellulosic biomass is a beneficial way to meet sustainability and energy security in the future. The main challenge in bioethanol conversion is the high cost of processing, in which enzymatic hydrolysis and fermentation are the major steps. Among the strategies to lower processing costs are utilizing both glucose and xylose sugars present in biomass for conversion. An approach featuring enzymatic hydrolysis and fermentation steps, identified as separate hydrolysis and fermentation (SHF was used in this work. Proposed solution is to use "pre-processing" technologies, including the thermal screw press (TSP and cellulose-organic-solvent based lignocellulose fractionation (COSLIF pretreatments. Such treatments were conducted on a widely available feedstock such as source separated organic waste (SSO to liberate all sugars to be used in the fermentation process. Enzymatic hydrolysis was featured with addition of commercial available enzyme, Accellerase 1500, to mediate enzymatic hydrolysis process. On average, the sugar yield from the TSP and COSLIF pretreatments followed by enzymatic hydrolysis was remarkable at 90%. In this work, evaluation of the SSO hydrolysate obtained from COSLIF and enzymatic hydrolysis pretreaments on ethanol yields was compared by fermentation results with two different recombinant strains: Zymomonas mobilis 8b and Saccharomyces cerevisiae DA2416. At 48 hours of fermentation, ethanol yield was equivalent to 0.48g of ethanol produced per gram of SSO biomass by Z.mobilis 8b and 0.50g of ethanol produced per gram of SSO biomass by S. cerevisiae DA2416. This study provides important insights for investigation of the source-separated organic (SSO waste on ethanol production by different strains and becomes a useful tool to facilitate future process optimization for pilot scale facilities.

  7. Comparative Study on the Typing of Killer Immunoglobulin-Like Receptor by Using Two Different Genotyping Methods%两种不同的KIR基因分型方法的对比研究

    Institute of Scientific and Technical Information of China (English)

    金士正; 甄建新; 何柳媚; 徐筠娉; 邓志辉

    2012-01-01

    目的 探讨常用的序列特异性引物-PCR(PCR-SSP)及流式序列特异性寡核苷酸探针(Flow-rSSO)杂交方法在杀伤细胞免疫球蛋白样受体(KIR)框架基因分型结果中的符合率和准确性.方法 随机选择2011年6月77例深圳造血干细胞捐献者的外周血样,分别用PCR-SSP及Flow-rSSO商品化试剂盒进行KIR框架基因定型,分别通过凝胶电泳图及HLA Fusion 2.0 Research软件分析法,分析结果的一致性.对初检结果不一致的样本,采用同一厂家、不同批号的商品化试剂盒进行复检,并采用美国国立卫生研究院癌症研究所KIR PCR-SSP方法进行检测.结果 77例样本中,75例完全一致,另2例(2.6%) KIR2DL2的结果不一致,PCR-SSP方法对KIR 2DL2的初检、复检检测结果均为阴性,但用Flow-rSSO Lot#004批号试剂盒检测结果为阳性.更换新的Flow-rSSO Lot# 005批号试剂盒复检,结果与PCR-SSP方法的检测结果相一致.结论 为保证KIR基因分型的准确性,开展KIR基因分型的室内、室间质控工作至关重要.%Objective To explore the accuracy of killer immunoglobulin-like receptor (KIR) genotyping by using the sequence specific primer-PCR (PCR-SSP)and flow reverse sequence specific oligonucleotide probe(Flow-rSSO)assays.Methods A total of 77 samples from randomly selected stem cell volunteer donors in Shenzhen were subjected to KIR genotyping using the commercial PCR-SSP and Flow-rSSO kits,respectively.The accordance of KIR genotyping results was evaluated and the reason causing the different results was analyzed in this study.Samples with inconclusive and different genotyping results were retested using the same PCR SSP and Flow-rSSO commercial kits with updated lot number and also typed by PCR-SSP method established by National Cancer Institute. Results In the 77 tested samples,the KIR genotypes of 75 samples were in accordance by PCR-SSP and Flow rSSO commercial kit. However,the other two samples showed different results

  8. 深圳毗邻香港依托珠三角关系辨析--与《珠三角重新整合与"龙头"城市之争》一文的商榷%On SZ's adjoining H.K and relying on Pearl River Delta——discussion with the article of "The reintegration of Pearl River Delta and the competition to be the head city

    Institute of Scientific and Technical Information of China (English)

    魏达志

    2004-01-01

    @@ 最近,拜读了刊登在2003年11月总第110期胡靖一文,一方面为作者对广州这座城市的热情和挚爱所感动;另一方面也对文章中所表达的大量的片面性观点表示惊讶.因为这篇文章(以下简称胡文)不仅语气上感性太多、理性不足;而且在论证上争论太多、学理太少;因此,有必要对胡文的若干观点作进一步的商榷、探讨或说澄清.

  9. Ruling of 15 April 1999 regarding the technical conditions of junction of the autonomous electric energy production installations to the HTA and BT public networks not connected to a large interconnected network; Arrete du 15 avril 1999 relatif aux conditions techniques de raccordement des installations de production autonome d'energie electrique aux reseaux publics HTA et BT non relies a un grand reseau interconnecte

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-06-15

    The document issued by the State Secretariat for Industry, concerning the technical conditions of junction of the autonomous electric energy production installations to the HTA and BT public networks not connected to a large interconnected network, takes into account the directive no.98/34/CE of European Parliament and Council of 22 June 1998, the notification no.98/0477/F, as well as a number of laws, decrees and rulings concerning the energy distribution and supply. The present ruling contains five articles stipulating the conditions of junction to the public networks. An appendix is given, regarding the junction to HTA and BT networks. It contains the following seven sections: 1. Field of application; 2. Administration in case of normal and perturbed regime; 3. Functioning of the signal transmission (175 Hz or another close frequency); 4. Decoupling protections; 5. Network management and control; 6. Participation in primary frequency tuning; 7. Participation in the local voltage setting. Also two informative appendices are given. The first one presents the characteristics of insular networks in Guadeloupe and the neighbouring islands, Martinique, Reunion, Guiana and Corsica. The second informative appendix gives the information necessary to be provided in the frame of network management and control. A table presents the information to be supplied to the power station and the control center, respectively, according to the section five of the first appendix.

  10. The involvement of bacterial quorum sensing in the spoilage of refrigerated Litopenaeus vannamei.

    Science.gov (United States)

    Zhu, Suqin; Wu, Haohao; Zeng, Mingyong; Liu, Zunying; Wang, Ying

    2015-01-02

    Quorum-sensing signals in refrigerated shrimp (Litopenaeus vannamei) undergoing spoilage were examined using bioreporter assays, thin-layer chromatography and gas chromatography-mass spectrometry, and the results revealed the presence of three types of autoinducers including acetylated homoserine lactones (AHLs) (i.e., N-hexanoyl-homoserine lactone, N-oxohexanoyl-homoserine lactone and N-octanoyl-homoserine lactone), autoinducer-2, and cyclic dipeptides (i.e., cyclo-(L-Pro-L-Leu), cyclo-(L-Leu-L-Leu) and cyclo-(L-Pro-L-Phe)). Autoinducer-2, rather than any AHL, was detected in extracts from pure cultures of the specific spoilage organisms (SSO), i.e., Shewanella putrefaciens (SS01) and Shewanella baltica (SA02). As for the cyclic peptides, only SA02 was determined to produce cyclo-(L-Pro-L-Leu). According to the transcription levels of LuxR (the master quorum-sensing regulator) in the SSO in response to exogenous autoinducers, the SSO could sense AHLs and cyclo-(L-Leu-L-Leu), rather than autoinducer-2, cyclo-(L-Leu-L-Leu) and cyclo-(L-Pro-L-Phe). In accordance with the results of LuxR expression, the production of biofilm matrixes and extracellular proteases in the SSO was regulated by exogenous AHLs and cyclo-(L-Pro-L-Leu), rather than 4,5-dihydroxy-2,3-pentanedione (the autoinducer-2 precursor), cyclo-(L-Leu-L-Leu) and cyclo-(L-Pro-L-Phe). Exogenous N-hexanoyl-homoserine lactone and cyclo-(L-Pro-L-Leu) increased the growth rates and population percentages of the SSO in shrimp samples under refrigerated storage, and interestingly, exogenous 4,5-dihydroxy-2,3-pentanedione also increased the population percentages of the SSO in vivo by inhibiting the growth of the competing bacteria. However, according to the levels of TVB-N and the volatile organic components in the shrimp samples, exogenous 4,5-dihydroxy-2,3-pentanedione did not accelerate the shrimp spoilage process as N-hexanoyl-homoserine lactone and cyclo-(L-Pro-L-Leu) did. In summary, our results suggest that

  11. Research on fatigue life expenditure and torsional vibration check of a 600 MW unit under subsynchronous oscillation%600 MW机组次同步振荡下轴系扭振校核及疲劳寿命损耗计算

    Institute of Scientific and Technical Information of China (English)

    崔亚辉; 张俊杰; 顾煜炯; 金铁铮; 孙鹏; 渠福来; 范景利

    2016-01-01

    Low-amplitude subsynchronous oscillation (SSO)problem occurred frequently during operation of a supercritical 600 MW unit.To solve this problem,the Riccati transfer matrix method was applied to es-tablish lumped-mass model for this unit.The torsional vibration natural frequency of shafting was solved, and the result was compared with the actual measured torsional vibration natural frequency of shafting.It was proved that the calculation results agreed well with the actual measured results,this model could be used to analyze the torsional vibration of this unit.Moreover,the relative value of torsional vibration stress shafting was solved based on the torsional vibration mode of this unit shafting,and the dangerous section of shafting was determined under SSO.The relationship between speed difference of the unit head and the fa-tigue life of the most dangerous section under SSO was established,the torsional vibration safety and fa-tigue life expenditure of the unit when the low-amplitude SSO occurred were evaluated.It provides impor-tant references for the same type units to analyze SSO.%针对某超临界600 MW机组在运行过程中经常出现的低振幅次同步振荡(SSO)问题,本文采用Riccati传递矩阵法建立了该机组的集中质量模型,求解了轴系的扭振固有频率,并与轴系的实测扭振固有频率进行了对比,证明了该模型计算结果与实测结果相符,可用于机组扭振分析。同时,应用本文模型,根据该机组轴系的扭振振型求解了轴系的扭振应力相对值,确定了SSO下轴系的危险截面;建立了次同步振荡下机头转速差与最危险截面疲劳寿命之间的关系,可对该机组发生低振幅SSO时的扭振安全性和疲劳寿命损耗进行评估。

  12. A comprehensive sulfur and oxygen isotope study of sulfur cycling in a shallow, hyper-euxinic meromictic lake

    Science.gov (United States)

    Gilhooly, William P.; Reinhard, Christopher T.; Lyons, Timothy W.

    2016-09-01

    Mahoney Lake is a permanently anoxic and sulfidic (euxinic) lake that has a dense plate of purple sulfur bacteria positioned at mid-water depth (∼7 m) where free sulfide intercepts the photic zone. We analyzed the isotopic composition of sulfate (δ34SSO4 and δ18OSO4), sulfide (δ34SH2S), and the water (δ18OH2O) to track the potentially coupled processes of dissimilatory sulfate reduction and phototrophic sulfide oxidation within an aquatic environment with extremely high sulfide concentrations (>30 mM). Large isotopic offsets observed between sulfate and sulfide within the monimolimnion (δ34SSO4-H2S = 51‰) and within pore waters along the oxic margin (δ34SSO4-H2S > 50‰) are consistent with sulfate reduction in both the sediments and the anoxic water column. Given the high sulfide concentrations of the lake, sulfur disproportionation is likely inoperable or limited to a very narrow zone in the chemocline, and therefore the large instantaneous fractionations are best explained by the microbial process of sulfate reduction. Pyrite extracted from the sediments reflects the isotopic composition of water column sulfide, suggesting that pyrite buried in the euxinic depocenter of the lake formed in the water column. The offset between sulfate and dissolved sulfide decreases at the chemocline (δ34SSO4-H2S = 37‰), a trend possibly explained by elevated sulfate reduction rates and inconsistent with appreciable disproportionation within this interval. Water column sulfate exhibits a linear response in δ18OSO4-δ34SSO4 and the slope of this relationship suggests relatively high sulfate reduction rates that appear to respond to seasonal changes in the productivity of purple sulfur bacteria. Although photosynthetic activity within the microbial plate influences the δ18OSO4-δ34SSO4 relationship, the biosignature for photosynthetic sulfur bacteria is restricted to the oxic/anoxic transition zone and is apparently minor relative to the more prevalent process of

  13. Imaging riometer observation in South Atlantic Anomaly

    Science.gov (United States)

    Makita, Kazuo; Hoshino, Mituo; Kato, Yasuo; Masanori, Nishino; Schuch, Nelson Jorge; Foppiano, Alberto J.; Ovalle, Ellias; Tanaka, Yoshi; Monreal, Ricardo

    The total geomagnetic field intensity is especially weak around the southern part of Latin America. This region is called South Atlantic Anomaly (SAA). We named this singularity region as "Geomagnetic Hole". High energetic particles larger than 100keV are precipitating into Geomagnetic Hole from Radiation belt. Since the intensity of Geomagnetic Hole is sharply decreasing now, the quantity of particle precipitation in this region will become more large near future. In order to examine Geomagnetic Hole phenomena, imaging riometer, photometer and other instruments are installed at INPE southern space observatory (SSO) and started observation since 1997. We also installed similar instruments and continue the observation at Concepcion / Punta Arenas in Chile and Kakioka in Japan. We study Cosmic Noise Absorption (CNA) obtained by imaging riometer and their relationships to particle data by GOES Satellite. From our analysis results, typical CNA events are observed in associated with big geomagnetic storm period at SSO and GOES particle date shows sharp decrease of energetic particle in this time. It suggests that radiation belt particles are precipitating to Geomagnetic Hole during ge-omagnetic storm period. Sometimes, CNA events are also observed during big substorm period and during the X-ray flare event. We also examined Punta Arenas, Concepcion and Kakioka imaging riometer data during geomagnetic storm time. However, typical CNA corresponding to SSO event is not found in our analysis. Our preliminary result suggests that particle precip-itation region is confined in a limited area around SSO, Brazil. On the other hands, Traveling Ionosphere Disturbance (TID) events are also observed at Concepcion and Kakioka as well as SSO by imaging riometer. Generally, number density of ionosphere electron increases and electron density disturbance with stripe-like structures are traveling during TID event. From imaging riometer observation, similar stripe-like CNA structure are

  14. Spectroscopic studies of Yb3+-doped rare earth orthosilicate crystals

    Science.gov (United States)

    Campos, S.; Denoyer, A.; Jandl, S.; Viana, B.; Vivien, D.; Loiseau, P.; Ferrand, B.

    2004-06-01

    Infrared transmission and Raman scattering have been used to study Raman active phonons and crystal-field excitations in Yb3+-doped yttrium, lutetium and scandium orthosilicate crystals (Y2SiO5 (YSO), Lu2SiO5 (LSO) and Sc2SiO5 (SSO)), which belong to the same C2h6 crystallographic space group. Energy levels of the Yb3+ ion 2F5/2 manifold are presented. In the three hosts, Yb3+ ions experience high crystal field strength, particularly in Yb:SSO. Satellites in the infrared transmission spectra have been detected for the first time in the Yb3+-doped rare earth orthosilicates. They could be attributed to perturbed Yb3+ sites of the lattices or to magnetically coupled Yb3+ pairs.

  15. Processes affecting δ34S and δ18O values of dissolved sulfate in alluvium along the Canadian River, central Oklahoma, USA

    Science.gov (United States)

    Tuttle, Michele L.W.; Breit, George N.; Cozzarelli, Isabelle M.

    2009-01-01

    The δ34S and δ18O values for dissolved sulfate in groundwater are commonly used in aquifer studies to identify sulfate reservoirs and describe biogeochemical processes. The utility of these data, however, often is compromised by mixing of sulfate sources within reservoirs and isotope fractionation during sulfur redox cycling. Our study shows that, after all potential sulfate sources are identified and isotopically characterized, the δ34SSO4 and δ18OSO4 values differentiate processes such as sulfate-source mixing, sulfide oxidation, barite dissolution, and organosulfur decomposition. During bacterial reduction of sulfate, the values reflect kinetic sulfur isotope fractionation and exchange of oxygen isotopes between sulfate and water. Detailed analysis of the chemistry (Cl and SO4 concentrations) and isotopic composition (δ2HH2Oand δ18OH2O) of groundwater in an alluvial aquifer in Central Oklahoma, USA allowed the identification of five distinct end members that supply water to the aquifer (regional groundwater flowing into the study area, river water, leachate from a closed landfill that operated within the site, rain, and surface runoff). The δ34SSO4 and δ18OSO4 values in each end member differentiated three sources of sulfate: sulfate dissolved from Early to Late Permian rocks within the drainage basin (δ34SSO4 = 8–12‰ and δ18OSO4 = 10‰), iron sulfides oxidized by molecular oxygen during low water-table levels (δ34SSO4 = − 16‰ and δ18OSO4 = 10‰), and organosulfur compounds (predominately ester sulfates) from decomposition of vegetation on the surface and from landfill trash buried in the alluvium (δ34SSO4 = 8‰ and δ18OSO4 = 6‰). During bacterial reduction of these sulfate sources, similar isotope fractionation processes are recorded in the parallel trends of increasing δ34SSO4 and δ18OSO4 values. When extensive reduction occurs, the kinetic sulfur isotope fractionation (estimated by εH2S–SO4 = − 23

  16. Annual Site Environmental Report: 2015 (ASER) for the SLAC National Accelerator Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Sabba, Dellilah [SLAC National Accelerator Lab., Menlo Park, CA (United States)

    2016-09-01

    This report, prepared by the SLAC National Accelerator Laboratory (SLAC) for the U.S. Department of Energy (DOE), SLAC Site Office (SSO), provides a comprehensive summary of the environmental program activities at SLAC for calendar year 2015. Annual Site Environmental Reports (ASERs) are prepared for all DOE sites with significant environmental activities, and distributed to relevant external regulatory agencies and other interested organizations or individuals. To the best of my knowledge, this report accurately summarizes the results of the 2015 environmental monitoring, compliance, and restoration programs at SLAC. This assurance can be made based on SSO and SLAC review of the ASER, and quality assurance protocols applied to monitoring and data analyses at SLAC.

  17. 基于Liferay门户与Lotus Domino OA单点登录系统的实现%Implementation of Single Sign-On system Based on Liferay Portal and Lotus Domino OA

    Institute of Scientific and Technical Information of China (English)

    郭成宝

    2014-01-01

    Achieving the SSO(Single Sign-On) between Liferay Portal and Lotus Domino OA, with the advantages of Liferay platform and Domino platform, then we can have better collaboration. Liferay Portal is the effective platform that is running for ofifce automation. This paper introduces the implementation of SSO and the key technologies were discussed.%本文介绍了Liferay门户与lotus Domino OA服务器的单点登录的实现,系统介绍Liferay门户应用优势和Lotus Domino平台在企业协作方面的优势,描述了单点登录认证方式的实现原理,并对其关键技术进行了讨论。

  18. Determination of DQB1 alleles using PCR amplification and allele-specific primers.

    Science.gov (United States)

    Lepage, V; Ivanova, R; Loste, M N; Mallet, C; Douay, C; Naoumova, E; Charron, D

    1995-10-01

    Molecular genotyping of HLA class II genes is commonly carried out using polymerase chain reaction (PCR) in combination with sequence-specific oligotyping (PCR-SSO) or a combination of the PCR and restriction fragment length polymorphism methods (PCR-RFLP). However, the identification of the DQB1 type by PCR-SSO and PCR-RFLP is very time-consuming which is disadvantageous for the typing of cadaveric organ donors. We have developed a DQB1 typing method using PCR in combination with allele-specific amplification (PCR-ASA), which allows the identification of the 17 most frequent alleles in one step using seven amplification mixtures. PCR allele-specific amplification HLA-DQB1 typing is easy to perform, and the results are easy to interpret in routine clinical practice. The PCR-ASA method is therefore better suited to DQB1 typing for organ transplantation than other methods.

  19. Trusted computing strengthens cloud authentication.

    Science.gov (United States)

    Ghazizadeh, Eghbal; Zamani, Mazdak; Ab Manan, Jamalul-lail; Alizadeh, Mojtaba

    2014-01-01

    Cloud computing is a new generation of technology which is designed to provide the commercial necessities, solve the IT management issues, and run the appropriate applications. Another entry on the list of cloud functions which has been handled internally is Identity Access Management (IAM). Companies encounter IAM as security challenges while adopting more technologies became apparent. Trust Multi-tenancy and trusted computing based on a Trusted Platform Module (TPM) are great technologies for solving the trust and security concerns in the cloud identity environment. Single sign-on (SSO) and OpenID have been released to solve security and privacy problems for cloud identity. This paper proposes the use of trusted computing, Federated Identity Management, and OpenID Web SSO to solve identity theft in the cloud. Besides, this proposed model has been simulated in .Net environment. Security analyzing, simulation, and BLP confidential model are three ways to evaluate and analyze our proposed model.

  20. Trusted Computing Strengthens Cloud Authentication

    Directory of Open Access Journals (Sweden)

    Eghbal Ghazizadeh

    2014-01-01

    Full Text Available Cloud computing is a new generation of technology which is designed to provide the commercial necessities, solve the IT management issues, and run the appropriate applications. Another entry on the list of cloud functions which has been handled internally is Identity Access Management (IAM. Companies encounter IAM as security challenges while adopting more technologies became apparent. Trust Multi-tenancy and trusted computing based on a Trusted Platform Module (TPM are great technologies for solving the trust and security concerns in the cloud identity environment. Single sign-on (SSO and OpenID have been released to solve security and privacy problems for cloud identity. This paper proposes the use of trusted computing, Federated Identity Management, and OpenID Web SSO to solve identity theft in the cloud. Besides, this proposed model has been simulated in .Net environment. Security analyzing, simulation, and BLP confidential model are three ways to evaluate and analyze our proposed model.