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Sample records for durissus terrificus cascavel

  1. Transferência do Hepatozoon tupinambis, parasita do lagarto Tupinambis teguixin, para a serpente cascavel (Crotalus durissus terrificus, por intermédio do mosquito Culex fatigans

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    Samuel B. Pessôa

    1974-01-01

    Full Text Available Os autores obtiveram a transferência do Hepatozoon tupinambis (Laveran e Salibeni, 1909 parasita do lagarto Teiidae, Tupinambis teguixin, L., para a serpente cascavel, Crotalus durissus terrificus (Laur., alimentando-a com mosquitos experimentalmente infectados. o parasita mantém os seus caracteres morfológicos no animal receptor, nos limites do tempo observado (cerca de 100 dias. O ofídio receptor apresentou cistos esquizogônicos do fígado.The authors achieved a transfer of the Hepatozoon tupinambis (Laveran and Salibeni, 1909, a parasite of the Sauria: Tupinambis teguixin, L., to a rattlesnake of the species Crotalus durissus terrificus, feeding the latter with experimentally infected mosquitoes. The parasite maintained its morphological characteristics in the recepting animal throughout the whole observation period (about 100 days. Schizogonic cysts were found in the liver of the rattlesnake.

  2. Clonagem, identificação e análise de genes de peptídeos tóxicos da cascavel sul americana, Crotalus durissus terrificus. Implicações evolutivas e funcionais

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    Gandhi Rádis Baptista

    2002-01-01

    O veneno de animais contém um arsenal de toxinas que desencadeia respostas fisiológicas e bioquímicas específicas. A crotamina, um peptídio catiônico (4,4 kDa, pI 9,5), é um dos componentes mais abundantes do veneno de cascavel Sul Arrericana (Crotalus durissus terrificus). No Brasil, há populações de C. d. terrificus que expressam ou não a crotamina no veneno. Em um único espécime de C. d. terrificus crotamina-positivo, foram isolados cDNAs precursores de duas isoformas de crotamina, dentre ...

  3. Perfil eletroforético das proteínas séricas de serpentes Crotalus durissus terrificus (cascavel criadas em cativeiro Serum protein electrophoresis profile of the rattlesnake Crotalus durissus terrificus kept in captivity

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    Joandes Henrique Fonteque

    2009-06-01

    Full Text Available As serpentes peçonhentas dos gêneros Bothrops e Crotalus têm sido mantidas em cativeiro visando à extração de venenos para a produção de imunobiológicos. O conhecimento da fisiologia desses animais e as alterações na concentração de proteínas e suas frações séricas são importantes para a identificação precoce de importantes enfermidades que cursam com estados de hipoproteinemia e hiperproteinemia. O objetivo do trabalho foi determinar a concentração de proteína total e o perfil eletroforético das proteínas séricas de serpentes Crotalus durissus terrificus (cascavel criadas em cativeiro. Foram colhidas amostras de sangue da veia coccígea ventral de 21 serpentes adultas e sadias, divididas em dois grupos: Grupo 1 de 12 machos com peso médio de 588,89±193,55g, e Grupo 2 de nove fêmeas com peso médio de 708,33±194,04g. A proteína total sérica foi determinada pelo método de refratometria e a eletroforese em gel de agarose. Obtiveram-se valores da proteína total sérica (g/dL de 4,51±0,50 para machos e de 4,82±0,72 para fêmeas, e para machos e fêmeas de 4,64±0,61. Foram identificadas pela eletroforese quatro frações protéicas (g/dL: albumina, a, b, g-globulinas e calculada a relação albumina:globulina. As serpentes fêmeas apresentaram maiores valores para as variáveis, albumina e para a relação albumina/globulina (AG diferindo significativamente (PThe poisonous snakes of the genera Crotalus and Bothrops have been kept in captivity with the purpose of extracting poison for the production of immunobiological. Knowledge of the physiology of these animals and serum proteins concentration changes are important for early identification of major diseases which lead to states of hypoproteinemia and hyperproteinemia. The objective was to determine the concentration of total protein and serum protein electrophoresis profile of Crotalus durissus terrificus (rattlesnake in captivity. Blood samples were taken from

  4. Evaluation of contraction in isolated corpus cavernosum from Crotalus durissus terrificus

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    Renata Lopes Rodrigues

    2013-01-01

    Resumo: O fato da cópula em serpentes ser muito prolongada, chegando a mais de 20 horas, sem que os mecanismos farmacológicos estejam conhecidos, motivou o nosso grupo a estudar o hemipênis de Crotalus durissus terrificus (cascavel). Diferentemente do observado em corpo cavernoso de outros mamíferos, o relaxamento induzido pela estimulação elétrica foi mais duradouro, além de resistente a ação da neurotoxina tetrodotoxina (TTX) em hemipênis de Crotalus. Uma hipótese inicial aventada foi que e...

  5. Afibrinogenemia following snake bite (Crotalus durissus terrificus

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    C. F. S. Amaral

    1988-08-01

    Full Text Available This paper reports two cases of afibrinogenemia with normal platelet count following Crotalus durissus terrificus, snake bite Both patients presented high output acute renal failure and case two also had increased blood levels of CPK and LDH compatible with the diagnosis of rhabdomyolysis. Case one was given an unknown amount of antivenom and was treated with epsilonaminocaproic acid and a fresh whole blood transfusion and showed recovery of the coagulation disturbance 40 hours following these measures. Case two was given an adequate amount of crotalide antivenom and the coagulation tests performed 12 hours later showed a normal partial thromboplastin time and fibrinogen 86 mg/100ml. Case one presented no haemorrhagic disturbances. Case two presented persistent bleeding following venopuncture and after removal of impetigo crust in the legs. Acute renal failure was treated conservatively and both patients were discharged from the hospital with recovery of the renal function.

  6. Compartment syndrome after South American rattlesnake (Crotalus durissus terrificus) envenomation.

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    Bucaretchi, F; De Capitani, E M; Hyslop, S; Mello, S M; Fernandes, C B; Bergo, F; Nascimento, F B P

    2014-07-01

    In order to report the outcome of a patient who developed compartment syndrome after South American rattlesnake (Crotalus durissus terrificus) envenomation, confirmed by subfascial pressure measurement and magnetic resonance imaging (MRI). A 63-year-old male was admitted 1 h after being bitten on the right elbow by a "large" snake, which was not brought for identification. Physical and laboratory features upon admission revealed two fang marks, local tense swelling, paresthesia, intense local pain, hypertension, coagulopathy, and CK = 1530 U/L (RV bite revealed generalized myalgia, muscle weakness, palpebral ptosis, and severe rhabdomyolysis (CK = 126,160 U/L) compatible with envenoming by C. d. terrificus. The patient was then treated with crotalic antivenom (200 mL, intravenously), fluid replacement, and urine alkalinization. Twenty-four-hour post-bite MRI showed marked muscular edema in the anterior compartment of the right forearm, with a high subfascial pressure (40 mmHg) being detected 1 h later. ELISA of a blood sample obtained upon admission, before antivenom infusion, revealed a high serum concentration of C. d. terrificus venom. No fasciotomy was performed and the patient was discharged seven days later without sequelae. Snakebite by C. d. terrificus with subfascial venom injection may lead to increased intracompartmental pressure.

  7. Crotalus durissus terrificus venom as a source of antitumoral agents

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    MA Soares

    2010-01-01

    Full Text Available The basic knowledge on neoplasms is increasing quickly; however, few advances have been achieved in clinical therapy against tumors. For this reason, the development of alternative drugs is relevant in the attempt to improve prognosis and to increase patients' survival. Snake venoms are natural sources of bioactive substances with therapeutic potential. The objective of this work was to identify and characterize the antitumoral effect of Crotalus durissus terrificus venom (CV and its polypeptide, crotoxin, on benign and malignant tumors, respectively, pituitary adenoma and glioblastoma. The results demonstrated that CV possess a powerful antitumoral effect on benign (pituitary adenoma and malignant (glioblastoma multiforme tumors with IC50 values of 0.96 ± 0.11 µg/mL and 2.15 ± 0.2 µg/mL, respectively. This antitumoral effect is cell-cycle-specific and dependent on extracellular calcium, an important factor for crotoxin phospholipase A2 activity. The CV antitumoral effect can be ascribed, at least partially, to the polypeptide crotoxin that also induced brain tumor cell death. In spite of the known CV nephrotoxicity and neurotoxicity, acute treatment with its antitumoral dose established in vitro was not found to be toxic to the analyzed animals. These results indicate the biotechnological potential of CV as a source of pharmaceutical templates for cancer therapy.

  8. DSC analysis of irradiated proteins from Crotalus durissus terrificus

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    Oliveira, Karina Corleto de; Silva, Monica Nascimento da; Goncalves, Karina de Oliveira; Spencer, Patrick Jack; Nascimento, Nanci do [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    Full text: Snake bites are a serious public health problem, especially in subtropical countries. In Brazil, the serum, the only effective treatment in case of snake bites, is produced in horses which, despite the large size, have a reduced lifespan due to the high toxicity of the antigen. It is known that ionizing radiation effects - direct and indirect - can modify the molecular structure, affecting the biological properties of proteins. Ionizing radiation has been employed to attenuate the toxicity of snake venoms, aiming to generate an improved antigen with low toxicity. Two proteins, purified from Crotalus durissus terrificus (Cdt) venom were tested in this work: crotoxin and crotamine. Crotoxin, the main toxic compound of Cdt venom, is a heterodimeric protein composed of two subunits: crotapotin and phospholipase A2. Crotamine is a highly basic polypeptide (pI - 10.3), with myotoxic activity and molecular weight of 4882 Da. It is composed of 42 amino acids residues and reticulated by three disulfide bonds. This study aimed to investigate the effects of radiation on crotoxin and crotamine using Differential Scanning Calorimetry (DSC). After isolation of the toxins by chromatographic techniques, they were irradiated with 2.0 kGy from {sup 60}Co source. The thermodynamics analysis, carried out in a METTLER TOLEDO, DSC 822e calorimeter, showed that irradiation promoted changes of the calorimetric profile. These changes suggest that, although radiation induced structural modifications of the protein, denaturation was only partial, since transition states could still be detected, suggesting that some structural elements were still present after irradiation. Taken together, our data suggest that following irradiation, the molecules underwent conformational changes, and that the remaining structural elements displayed a lower enthalpy, clearly indicating that the previously described loss of toxicity of irradiated toxins can be mostly ascribed to structural changes

  9. Histopathological evaluation in experimental envenomation of dogs with Crotalus durissus terrificus venom

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    F. Sangiorgio

    2008-01-01

    Full Text Available The present work evaluated histopathological aspects in experimental envenomation of dogs with Crotalus durissus terrificus venom. Twenty-eight mixed breed adult dogs were divided into three groups of seven animals each: Group I - only venom; Group II - venom + 50ml antiophidic serum + fluid therapy; Group III - venom + 50ml antiophidic serum + fluid therapy + urine alkalization. Lyophilized venom of Crotalus durissus terrificus was reconstituted in saline solution and inoculated subcutaneously at the dose of 1mg/kg body weight. Three animals of each group were subjected to euthanasia, and their muscular tissue, brain, spleen, kidneys, heart, lungs, stomach, small and large intestines, and popliteal lymph node fragments were collected for histopathological evaluation. There was myonecrosis in the inoculated limb, renal tubular degeneration, lymphoid hyperplasia of spleen, and unspecific reactive hepatitis. These results show the antigenicity and action of the venom on the immune system.

  10. Epidemiologia e quadro clínico do acidente por cascavel sul-americana (Crotalus durissus Epidemiology and clinical features of South American rattlesnakes (Crotalus durissus envenomation

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    Miguel Tanús Jorge

    1992-08-01

    Full Text Available Foram estudados 249 casos de acidentes por cascavel sul-americana (Crotalus durissus atendidos no HVB-IB, São Paulo, Brasil, de 1974 a 1990. Os acidentes foram mais comuns no período da tarde, nos meses de janeiro a abril e de outubro a dezembro. Dentre cem serpentes classificadas quanto à subespécie 99 eram C. d. terrificus. Pertenciam ao sexo masculino 80,7% dos pacientes. Os membros inferiores e superiores foram picados em, respectivamente, 66,4% e 29,2% dos casos. As manifestações clínicas mais freqüentes foram dor (61,0% e edema (55,0% no local da picada, ptose palpebral (75,9%, escurecimento da urina atribuível à mioglobinúria (38,6% e mialgia (36,1%. Nove pacientes foram submetidos a diálise devido a insuficiência renal aguda (3,6%, três apresentavam insuficiência respiratória que motivou intubação e/ou traqueostomia e um apresentou acidente vascular cerebral isquêmico. A alteração da coagulação sanguínea ocorreu em 48,1% dos pacientes. Oito amostras de sangue colhidas horas após a picada mostraram leucocitose sendo 6 com desvio a esquerda e, nos dias subseqüentes, tendência à normalização do número de leucócitos e aparecimento de eosinofilia. Atividade sérica da creatinoquinase apresentou-se aumentada em 20 dentre 21 pacientes, sendo maior no final das primeiras 24 horas após a picada, chegando a 2.377 vezes o valor de referência. A letalidade foi de 0,8%.Under study were two hundred and forty nine cases of accidents caused by South American rattlesnakes (Crotalus durissus admitted to the Vital Brasil Hospital, São Paulo, Brasil, from 1974 to 1990. The accidents were more common in the afternoon, from January to April and from October to December. Ninety nine out of 100 snakes classified as subspecies were C. d. terrificus. Most of the patients were males (80.7%. The inferior and superior limbs were bitten in 66.4% and 29.2% of the cases, respectively. The more frequent clinical manifestations were

  11. Purification and radiodination of gyroxin, toxin like trombin, of crotalus durissus terrificus venom; Purificacao e radioiodacao da giroxina (toxina semelhante a trombina) do veneno de crotalus durissus terrificus

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    Camillo, Maria A.P.; Paes, Paulo C.A.; Ribela, Maria T.C.P.; Rogero, Jose R. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)

    1997-12-01

    Snake`s venoms have attracted special interest in research because they are rich in bioactive compounds, which are scientific tools to study many physiological processes and are also important source of new therapeutic agents. Some thrombin-like enzymes are used as a reagent in laboratorial assays as well as in medical drugs. However, a neurotoxic syndrome called gyroxin syndrome or barrel rotation seems to be related to those enzymes. Pharmacokinetics and biodistribution studies of these toxins can contribute to clarifly this mechanisms and consequently, it will help to elaborate safer clinical prescriptions giving more information about possible nocive effects. This paper describes the first step of these studies with the thrombin-like enzyme (or gyroxin) from Crotalus durissus terrificus`s venom. The purification and radioiodination methods as well as electrophoretic analysis and biological assay are presented. (author). 9 refs., 5 figs., 1 tab.

  12. Pulmonary mechanic and lung histology injury induced by Crotalus durissus terrificus snake venom.

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    Nonaka, Paula Naomi; Amorim, César Ferreira; Paneque Peres, Ana Claudia; E Silva, César Augusto Melo; Zamuner, Stella R; Ribeiro, Wellington; Cogo, José Carlos; Vieira, Rodolfo P; Dolhnikoff, Marisa; de Oliveira, Luis Vicente Franco

    2008-06-01

    In the present work we investigated the effects of Crotalus durissus terrificus venom (CdtV) on the pulmonary mechanic events [static and dynamic elastance, resistive (DeltaP1) and viscoelastic pressures (DeltaP2)] and histology after intramuscular injection of saline solution (control) or venom (0.6 microg/g). The static and dynamic elastance values were increased significantly after 3 h of venom inoculation, but were reduced at control values in the other periods studied. The DeltaP1 values that correspond to the resistive properties of lung tissue presented a significant increase after 6h of CdtV injection, reducing to basal levels 12h after the venom injection. In DeltaP2 analysis, correspondent to viscoelastic components, an increase occurred 12 h after the venom injection, returning to control values at 24 h. CdtV also caused an increase of leukocytes recruitment (3-24 h) to the airways wall as well as to the lung parenchyma. In conclusion, C. durissus terrificus rattlesnake venom leads to lung injury which is reverted, after 24 h of inoculation.

  13. The Evolutionary Implications of Hemipenial Morphology of Rattlesnake Crotalus durissus terrificus (Laurent, 1768 (Serpentes: Viperidae: Crotalinae.

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    Marcovan Porto

    Full Text Available Most amniotes vertebrates have an intromittent organ to deliver semen. The reptile Sphenodon and most birds lost the ancestral penis and developed a cloaca-cloaca mating. Known as hemipenises, the copulatory organ of Squamata shows unique features between the amniotes intromittent organ. They are the only paired intromittent organs across amniotes and are fully inverted and encapsulated in the tail when not in use. The histology and ultrastructure of the hemipenes of Crotalus durissus rattlesnake is described as the evolutionary implications of the main features discussed. The organization of hemipenis of Crotalus durissus terrificus in two concentric corpora cavernosa is similar to other Squamata but differ markedly from the organization of the penis found in crocodilians, testudinata, birds and mammals. Based on the available data, the penis of the ancestral amniotes was made of connective tissue and the incorporation of smooth muscle in the framework of the sinusoids occurred independently in mammals and Crotalus durissus. The propulsor action of the muscle retractor penis basalis was confirmed and therefore the named should be changed to musculus hemipenis propulsor.The retractor penis magnus found in Squamata has no homology to the retractor penis of mammals, although both are responsible for the retraction of the copulatory organ.

  14. Kinetic characterization of gyroxin, a serine protease from Crotalus durissus terrificus venom.

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    Yonamine, Camila M; Kondo, Marcia Y; Juliano, Maria A; Icimoto, Marcelo Y; Baptista, Gandhi R; Yamane, Tetsuo; Oliveira, Vitor; Juliano, Luis; Lapa, Antônio J; Lima-Landman, Maria Teresa R; Hayashi, Mirian A F

    2012-12-01

    This work describes for the first time the characterization of the enzymatic features of gyroxin, a serine protease from Crotalus durissus terrificus venom, capable to induce barrel rotation syndrome in rodents. Measuring the hydrolysis of the substrate ZFR-MCA, the optimal pH for proteolytic cleavage of gyroxin was found to be at pH 8.4. Increases in the hydrolytic activity were observed at temperatures from 25 °C to 45 °C, and increases of NaCl concentration up to 1 M led to activity decreases. The preference of gyroxin for Arg residues at the substrate P1 position was also demonstrated. Taken together, this work describes the characterization of substrate specificity of gyroxin, as well as the effects of salt and pH on its enzymatic activity.

  15. Isolation and characterization of delta toxin from the venom of Crotalus durissus terrificus; Isolamento e caracterizacao da delta toxina do veneno de Crotalus durissus terrificus

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    Campos, Lucelia de Almeida

    2006-07-01

    The Crotalus durissus terrificus venom has been so far described as being of low complexity, with four major components described: convulxin, gyroxin, crotoxin and crotamine. In recent studies, other components of this venom were characterized as, for example, an analgesic factor. In 1980, Vital Brazil predicted the existence of a toxin which could be involved in platelet aggregation, and named it delta toxin. However, this toxin has never been isolated or characterized. The aim of the present work was to purify and characterize this toxin. After FPLC size exclusion chromatography followed by reverse phase HPLC, an homogeneous fraction was obtained, with a molecular weight of 14,074.92 Da. When analyzed by SOS-PAGE, this toxin presented an anomalous behavior, with a molecular weight of 14 kDa, while in 2D gels, spots around 40 kDa and with an isoelectrical point between 4 and 5 were observed suggesting isoforms with glicosilation microheterogeneity. After trypsin digestion, the fragments were submitted to the swissprot databank showing high homology (43% coverage, 15 matching peptides) with trocarin, a prothrombin activator from Tropidechis carinatus. These data were further confirmed by aminoacid analysis. The toxin was tested for its ability to activate factor II and X using synthetic substrates. Our data indicate a direct activation of factor X. The same toxin also behaved as a potent direct platelet aggregation activator on washed platelets. Assays with specific inhibitors indicate that neither metalloproteinase, nor serinoproteinase or t lectin domains are involved in the aggregating activity, since EDTA, benzamidin and D-galactose did not inhibit the toxin. In the present work, we were able to identify, purify and characterize a new toxin from the brazilian rattlesnake. It behaved as predicted by Vital-Brazil and displayed direct factor X activating properties, also inducing platelet aggregation, even at low concentrations. Our data also indicate that it is

  16. Effects of the Crotalus durissus terrificus snake venom on hepatic metabolism and oxidative stress.

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    da Silva, Jonas Golart; da Silva Soley, Bruna; Gris, Vanessa; do Rocio Andrade Pires, Amanda; Caderia, Silvia Maria Suter Correia; Eler, Gabrielle Jackin; Hermoso, Aparecida Pinto Munhos; Bracht, Adelar; Dalsenter, Paulo Roberto; Acco, Alexandra

    2011-01-01

    Snake venoms present different action mechanisms because of their complex composition, represented mainly by toxins and enzymes. This work aimed to investigate the effects of the Crotalus durissus terrificus(Cdt) venom in the liver. Wistar rats were inoculated intraperitoneally with saline (control) or Cdt venom. After 3, 4, or 6 h, the following parameters were analyzed: (a) hepatic function, (b) oxidative stress parameters, and (c) the metabolism of alanine in the isolated perfused liver. Plasma activities of alanine aminotransferase and aspartate aminotransferase and hepatic glutathione S-transferase and catalase presented significant elevation in rats inoculated with 300 μg ⋅ kg(-1) Cdt venom. Liver lipoperoxidation was enormously increased by venom doses of 100, 200, and 300 μg ⋅kg(-1) , whereas glutathione S-transferase was not changed. Perfused livers from rats inoculated with 1500 μg ⋅kg(-1) venom showed increased production of lactate, pyruvate, and ammonia when alanine was the metabolic substrate. These results demonstrate that the Cdt venom can produce several changes in hepatocytes. The causes of the changes are possibly related to the disequilibrium in the redox homeostasis but also to specific needs of the poisoned organism, for example, an increased supply of lactate and pyruvate in response to an increased activity of the Cori cycle. Copyright © 2010 Wiley Periodicals, Inc.

  17. American rattlesnake (Crotalus durissus terrificus bite accidents in dogs in Argentina

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    Koscinczuk P.

    2000-01-01

    Full Text Available The symptomatology and treatment of two dogs bitten by Crotalus durissus terrificus are described. Neurological signs were present few minutes after the accident with local anesthesia and ataxia of the affected limb and neurotoxic fascia. Alanine aminotransferase (ALT, aspartate aminotransferase (AST, creatinine kinase (CK, lactate dehydrogenase (LDH and calcium were evaluated in an attempt to investigate muscle damage. Renal failure was not observed but some alterations were detected in urine. Urine density was low and the urinary sediment contained granular clumps and small round cells. Muscle samples were obtained from both legs for histopathological study, showing edema and isolated necrotic fibers. Both dogs received treatment within four hours after the accident by intravenous route. The antivenom was administered diluted in 250ml of Ringer solution in a dose enough to inactivate more than 8mg of venom. Dexamethasone was applied previously to the antivenom. Clinical evolution was good and both patients were in good health condition on the second day after the accident.

  18. Pro- and Anti-Inflammatory Cytokines Release in Mice Injected with Crotalus durissus terrificus Venom

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    A. Hernández Cruz

    2008-01-01

    Full Text Available The effects of Crotalus durissus terrificus venom (Cdt were analyzed with respect to the susceptibility and the inflammatory mediators in an experimental model of severe envenomation. BALB/c female mice injected intraperitoneally presented sensibility to Cdt, with changes in specific signs, blood biochemical and inflammatory mediators. The venom induced reduction of glucose and urea levels and an increment of creatinine levels in serum from mice. Significant differences were observed in the time-course of mediator levels in sera from mice injected with Cdt. The maximum levels of IL-6, NO, IL-5, TNF, IL-4 and IL-10 were observed 15 min, 30 min, 1, 2 and 4 hours post-injection, respectively. No difference was observed for levels of IFN-γ. Taken together, these data indicate that the envenomation by Cdt is regulated both pro- and anti-inflammatory cytokine responses at time-dependent manner. In serum from mice injected with Cdt at the two first hours revealed of pro-inflammatory dominance. However, with an increment of time an increase of anti-inflammatory cytokines was observed and the balance toward to anti-inflammatory dominance. In conclusion, the observation that Cdt affects the production of pro- and anti-inflammatory cytokines provides further evidence for the role played by Cdt in modulating pro/anti-inflammatory cytokine balance.

  19. Thermolability of 28S ribosomal ribonucleic acid from the liver of Crotalus durissus terrificus (Ophidia, Reptilia).

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    Giorgini, J F; De Lucca, F L

    1973-09-01

    Instability of 28S rRNA of Crotalus durissus terrificus liver was observed during hotphenol extraction: purified 28S rRNA is converted into an 18S RNA component by heat treatment. It was also found that ;6S' and ;8S' low-molecular-weight RNA species were released during the thermal conversion. This conversion and the release of the low-molecular-weight species were also induced by 8m-urea and 80% (v/v) dimethyl sulphoxide at 0 degrees C. Evidence is presented that this phenomenon is an irreversible process and results from the rupture of hydrogen bonds. The 18S RNA product was shown to be homogeneous by polyacrylamide-gel electrophoresis and by sucrose-density-gradient centrifugation. The base composition of the 18S RNA products obtained by heat, urea or dimethyl sulphoxide treatments was similar. The C+G content of the 18S RNA product was different from that of the native 18S rRNA, but similar to that of 28S rRNA.

  20. Occurrence of Cryptosporidium (Apicomplexa, Cryptosporidiidae in Crotalus durissus terrificus (Serpentes, Viperidae in Brazil

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    Karasawa Andréa Satie Matsubara

    2002-01-01

    Full Text Available The objective of the present study was to investigate the prevalence of Cryptosporidium (Apicomplexa, Cryptosporidiidae in the snake Crotalus durissus terrificus (Serpentes, Viperidae. Fifty animals were evaluated for the presence of oocysts of Cryptosporidium sp. at the time of arrival and 30 and 60 days later. Intestinal washings with saline solution (1% body weight, fecal samples, and organ scrapings were collected during the study. Oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by a density gradient centrifugation technique. Smears were made with the sediment and submitted to modified acid-fast and auramine-rhodamine staining. Cryptosporidium-positive smears were used as controls for the experimental findings. The overall prevalence of Cryptosporidium sp. oocysts was 14%. Among the positive snakes, oocysts were detected only in the intestinal washing in two specimens, only in the feces in four specimens, and in both materials at least once in one specimen. The positive snakes were predominantly from Santa Maria da Serra city State of São Paulo (57.1%. We also observed that all of the examinations that presented positive results were obtained at least 27 days after the capture of the animals.

  1. Biochemical and biological evaluation of gyroxin isolated from Crotalus durissus terrificus venom

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    LC Barros

    2011-01-01

    Full Text Available Gyroxin, a thrombin-like enzyme isolated from Crotalus durissus terrificus venom and capable of converting fibrinogen into fibrin, presents coagulant and neurotoxic activities. The aim of the present study was to evaluate such coagulant and toxic properties. Gyroxin was isolated using only two chromatographic steps - namely gel filtration (Sephadex G-75 and affinity (Benzamidine Sepharose 6B - resulting in a sample of high purity, as evaluated by RP-HPLC C2/C18 and electrophoretic analysis that showed a molecular mass of 30 kDa. Gyroxin hydrolyzed specific chromogenic substrates, which caused it to be classified as a serine proteinase and thrombin-like enzyme. It was stable from pH 5.5 to 8.5 and inhibited by Mn²+, Cu²+, PMSF and benzamidine. Human plasma coagulation was more efficient at pH 6.0. An in vivo toxicity test showed that only behavioral alterations occurred, with no barrel rotation. Gyroxin was not able to block neuromuscular contraction in vitro, which suggests that its action, at the studied concentrations, has no effect on the peripheral nervous system.

  2. Functional and structural analysis of two fibrinogen-activating enzymes isolated from the venoms of Crotalus durissus terrificus and Crotalus durissus collilineatus

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    Daniela G.L.de Oliveira; Mário T.Murakami; Adelia C.O.Cintra; Jo(a)o J.Franco; Suely V.Sampaio; Raghuvir K.Arni

    2009-01-01

    Fibrinogen-activating enzymes,widely distributed in Crotalidae and Viperidae venoms,are single-chain glycosylated serine proteases that display high macromolecular selectivity and are often referred to as thrombin-like enzymes(TLEs).TLEs serve as structural models to extend our understanding of the structure-function relationships of blood coagulation factors,have been clinically used for the treatment of thrombotic diseases,and are used as tools in clinical assays.The combination of gel filtration and ion-exchange chromatography proved to be successful in obtaining milligram quantities of pure samples of TLEs from the venoms of Crotalus durissus terrificus(white venom)and Crotalus durissus collilineatus(yellow venom).Functional characterization indicates that both enzymes preferentially degrade the Bβ chain of bovine fibrinogen and possess edema-inducing and coagulant activities.However,the TLE from C.d.collilineatus venom shows twofold higher coagulant activity with a minimum coagulant dose(MCD)of 0.6 μg/μl,whereas the enzyme isolated from C.d terrificus indicated an MCD of 1.5μg/μl.Molecular modeling of gyroxin and structural comparisons with other highly conserved snake venom serine proteases,underlines the key role played by the surface charge distribution and the double insertion in the 174-surface loop in macromolecular substrate recognition by TLEs.

  3. Seeing beyond the tip of the iceberg: A deep analysis of the venome of the Brazilian Rattlesnake, Crotalus durissus terrificus

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    Rafael D. Melani

    2015-09-01

    Full Text Available The complete characterization of the snake venom protein components is a requirement for a systems-wide understanding of their biological context. In this work, we provide a deep proteomic characterization of Crotalus durissus terrificus venom using different bottom-up approaches. We identified more than five times more protein families than the sum of all identifications previously reported. For the first time in this sub-species, we report the identification of three new toxin families: CRISP, phospholipase-B, and SVVEGF. This work also describes proteins involved in regulation of toxin synthesis and processing that are present in venom.

  4. Intrahippocampal Infusion of Crotamine Isolated from Crotalus durissus terrificus Alters Plasma and Brain Biochemical Parameters

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    Rithiele Gonçalves

    2014-11-01

    Full Text Available Crotamine is one of the main constituents of the venom of the South American rattlesnake Crotalus durissus terrificus. Here we sought to investigate the inflammatory and toxicological effects induced by the intrahippocampal administration of crotamine isolated from Crotalus whole venom. Adult rats received an intrahippocampal infusion of crotamine or vehicle and were euthanized 24 h or 21 days after infusion. Plasma and brain tissue were collected for biochemical analysis. Complete blood count, creatinine, urea, glutamic oxaloacetic transaminase (GOT, glutamic pyruvic transaminase (GPT, creatine-kinase (CK, creatine kinase-muscle B (CK-MB and oxidative parameters (assessed by DNA damage and micronucleus frequency in leukocytes, lipid peroxidation and protein carbonyls in plasma and brain were quantified. Unpaired and paired t-tests were used for comparisons between saline and crotamine groups, and within groups (24 h vs. 21 days, respectively. After 24 h crotamine infusion promoted an increase of urea, GOT, GPT, CK, and platelets values (p ≤ 0.01, while red blood cells, hematocrit and leukocytes values decreased (p ≤ 0.01. Additionally, 21 days after infusion crotamine group showed increased creatinine, leukocytes, TBARS (plasma and brain, carbonyl (plasma and brain and micronucleus compared to the saline-group (p ≤ 0.01. Our findings show that crotamine infusion alter hematological parameters and cardiac markers, as well as oxidative parameters, not only in the brain, but also in the blood, indicating a systemic pro-inflammatory and toxicological activity. A further scientific attempt in terms of preserving the beneficial activity over toxicity is required.

  5. Tetrodotoxin-insensitive electrical field stimulation-induced contractions on Crotalus durissus terrificus corpus cavernosum.

    Science.gov (United States)

    Campos, Rafael; Mónica, Fabíola Z; Rodrigues, Renata Lopes; Rojas-Moscoso, Julio Alejandro; Moreno, Ronilson Agnaldo; Cogo, José Carlos; de Oliveira, Marco Antonio; Antunes, Edson; De Nucci, Gilberto

    2017-01-01

    Reptiles are the first amniotes to develop an intromitent penis, however until now the mechanisms involved in the electrical field stimulation-induced contraction on corpora cavernosa isolated from Crotalus durissus terrificus were not investigated. Crotalus and rabbit corpora cavernosa were mounted in 10 mL organ baths for isometric tension recording. Electrical field stimulation (EFS)-induced contractions were performed in presence/absence of phentolamine (10 μM), guanethidine (30 μM), tetrodotoxin (1 μM and 1mM), A-803467 (10 μM), 3-iodo-L-Tyrosine (1 mM), salsolinol (3 μM) and a modified Krebs solution (equimolar substitution of NaCl by N-methyl-D-glucamine). Immuno-histochemistry for tyrosine hydroxylase was also performed. Electrical field stimulation (EFS; 8 Hz and 16 Hz) caused contractions in both Crotalus and rabbit corpora cavernosa. The contractions were abolished by previous incubation with either phentolamine or guanethidine. Tetrodotoxin (1 μM) also abolished the EFS-induced contractions of rabbit CC, but did not affect EFS-induced contractions of Crotalus CC. Addition of A-803467 (10 μM) did not change the EFS-induced contractions of Crotalus CC but abolished rabbit CC contractions. 3-iodo-L-Tyrosine and salsolinol had no effect on EFS-induced contractions of Crotalus CC and Rabbit CC. Replacement of NaCl by N- Methyl-D-glucamine (NMDG) abolished EFS-induced contractions of rabbit CC, but did not affect Crotalus CC. The presence of tyrosine hydroxylase was identified in endothelial cells only of Crotalus CC. Since the EFS-induced contractions of Crotalus CC is dependent on catecholamine release, insensitive to TTX, insensitive to A803467 and to NaCl replacement, it indicates that the source of cathecolamine is unlikely to be from adrenergic terminals. The finding that tyrosine hydroxylase is present in endothelial cells suggests that these cells can modulate Crotalus CC tone.

  6. Interaction study of water radiolysis products with Crotalus durissus terrificus miotoxin; Estudo das interacoes dos produtos de radiolise da agua com a miotoxina do veneno de Crotalus durissus terrificus

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    Silva, Murilo Casare da

    2008-07-01

    Ionizing radiation has been satisfactorily employed for venoms detoxification. In this report, the radiation was employed to verify the effects caused by the radiolysis products of water on the Crotamine, toxin purified from Crotalus durissus terrificus venom. These effects were analyzed using some substances called 'scavengers', those substances competes for specific reactive species hindering them to act on the toxins molecules. In order to study the possible structural damages caused on the toxins, circular dichroism, fluorescence, nuclear magnetic resonance, amino acids analysis and intravital microscopy were employed. Our results indicate that ionizing radiation caused structure alterations, mainly, in secondary and tertiary structure of crotamine. In the irradiated crotamine, was not possible to determine tridimensional structure. And the crotamine toxic effect was removed by ionizing radiation. (author)

  7. Clinical and hematological alterations in dogs during experimental envenomation with Crotalus durissus terrificus venom and treated with antiophidic serum

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    R. M. B. Nogueira

    2006-04-01

    Full Text Available The present work aimed to evaluate the clinical and hematological aspects during experimental envenomation by Crotalus durissus terrificus in dogs treated with different antiophidic serum doses. Sixteen dogs were divided into two groups of eight animals each. Group I received 1mg/kg venom subcutaneously and 30mg antiophidic serum intravenously; Group II received 1mg/kg venom subcutaneously and 60mg antiophidic serum intravenously. In the clinical evaluation, we observed ataxia, moderate sedation, dilated pupils, sialorrhea, flaccid paralysis of mandibular muscles, and discreet edema at the site of venom inoculation. Evaluating red and white blood cells, we observed a decrease of hemoglobins, globular volume and erythrocytes, and an increase of plasmatic proteins, leukocytes, neutrophils, monocytes and lymphocytes. Clotting time increased and there was blood incoagulability with return to normal clotting time six hours after antiophidic serum administration. Animals treated with six antiophidic serum flasks had a faster recovery than the animals that received three serum flasks.

  8. Comparison of wildlife and captivity rattlesnakes (Crotalus durissus terrificus microbiota Comparação da microbiota de cascavéis (Crotalus durissus terrificus de vida-livre e cativeiro

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    Rui S. Ferreira Junior

    2009-12-01

    Full Text Available The study evaluated and compared the aerobic microbiota from the oral cavity, cloaca and venom of Crotalus durissus terrificus snakes, recently caught from the wild and kept under quarantine (WQ, individual captivity (IC and collective captivity (CC. Antimicrobial drug effectiveness on isolated agents also was assayed. From group I, II and III were isolated, respectively, 29 (63.04%, 38 (90.48% and 21 (42.86% microorganisms from the cloaca; 15 (32.61%, 3 (7.14% and 25 (51.02% microorganisms from the oral cavity; and, 2 (4.35%, 1 (2.38% and 3 (6.12% microorganisms from venom. The most frequent bacteria were Pseudomonas aeruginosa, Proteus vulgaris and Morganella morganii, with sensitivity to amikacin, gentamicin, norfloxacin, sulfazotrin and tobramycin. Snakes kept in semi-open captivity exhibited the fewest microorganisms in oral cavities, perhaps due to the environment in captivity, with different temperature gradients, running water, absence of daily handling, circulating air, possibility of moving around, daily cleaning, and sunlight access.Este estudo avaliou e comparou a microflora aeróbica da cavidade oral, cloaca e veneno de serpentes Crotalus durissus terrificus recém-capturadas da natureza e mantidas sob quarentena (WQ, mantidas em cativeiro coletivo (CC e em cativeiro individual (IC. A eficácia de drogas antimicrobianas de agentes isolados foi também avaliada. Foram isolados microorganismos dos grupos I, II e III respectivamente: 29 (63.04%, 38 (90.48% e 21 (42.86% da cloaca; 15 (32.61%, 3 (7.14% e 25 (51.02% da cavidade oral, e finalmente 2 (4.35%, 1 (2.38% e 3 (6.12% do veneno. As bactérias mais frequentes foram Pseudomonas aeruginosa, Proteus vulgaris e Morganella morganii, com sensibilidade para amikacina, gentamicina, norfloxacina, sulfazotrina e tobramicina. Serpentes mantidas no cativeiro semi-aberto mostraram menor número de agentes infecciosos em cavidade oral, talvez devido ao ambiente de cativeiro com diferentes

  9. Preliminary X-ray crystallographic studies of a tetrameric phospholipase A{sub 2} formed by two isoforms of crotoxin B from Crotalus durissus terrificus venom

    Energy Technology Data Exchange (ETDEWEB)

    Marchi-Salvador, D. P.; Corrêa, L. C.; Salvador, G. H. M.; Magro, A. J. [Departamento de Física e Biofísica, Instituto de Biociências, UNESP, CP 510, 18618-000 Botucatu-SP (Brazil); Oliveira, C. Z. [Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, FCFRP, USP, Ribeirão Preto-SP (Brazil); Iulek, J. [Departamento de Química, UEPG, Ponta Grossa-PR (Brazil); Soares, A. M. [Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, FCFRP, USP, Ribeirão Preto-SP (Brazil); Fontes, M. R. M., E-mail: fontes@ibb.unesp.br [Departamento de Física e Biofísica, Instituto de Biociências, UNESP, CP 510, 18618-000 Botucatu-SP (Brazil)

    2007-12-01

    Crotoxin B is a basic phospholipase A{sub 2} found in the venom of C. durissus terrificus and is one of the subunits that constitute crotoxin. Here, the crystallization, X-ray diffraction data collection and molecular-replacement solution of a novel tetrameric complex formed by two dimers of crotoxin B isoforms are presented. Crotoxin B is a basic phospholipase A{sub 2} found in the venom of Crotalus durissus terrificus and is one of the subunits that constitute crotoxin. This heterodimeric toxin, which is the main component of C. d. terrificus venom, is completed by an acidic, nontoxic and non-enzymatic component (crotoxin A) and is involved in important envenomation effects, such as neurological disorders, myotoxicity and renal failure. Although crotoxin was first crystallized in 1938, no crystal structure is currently available for crotoxin, crotoxin A or crotoxin B. In this work, the crystallization, X-ray diffraction data collection to 2.28 Å resolution and molecular-replacement solution of a novel tetrameric complex formed by two dimers of crotoxin B isoforms (CB1 and CB2) is presented.

  10. Single-step purification of crotapotin and crotactine from Crotalus durissus terrificus venom using preparative isoelectric focusing

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    Aguiar A.S.

    1997-01-01

    Full Text Available We describe the isolation of crotoxin, a presynaptic B-neurotoxin, as well as its subunits B (crotactine and A (crotapotin from lyophilized Crotalus durissus terrificus venom by a single-step preparative isoelectric focusing procedure. From 98 mg of dried venom protein 20.1 mg of crotactine and 13.1 mg of crotapotin were recovered in the first step of focalization and 4.2 mg in a second run. These values correspond to 35.7% of the total venom protein applied. Crotactine separated in the 9.3-7.0 pH range (tubes 1-6 and crotapotin in the 1.8-2.8 pH range (tubes 15-19 and both were homogeneous by SDS-PAGE and N-terminal amino acid analysis. Crotactine, a 12-kDa protein, presented hemolytic and phospholipase A2 activity. Thus, using isoelectric focusing we simultaneously purified both toxins in high yields. This method can be used as an alternative for the purification and characterization of proteins from other snake venoms under conditions in which biological activity is retained

  11. Crotoxin, the major toxin from the rattlesnake Crotalus durissus terrificus, inhibits ³H-choline uptake in guinea pig ileum

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    L.S. Kattah

    2000-09-01

    Full Text Available We examined the effect of crotoxin, the neurotoxic complex from the venom of the South American rattlesnake Crotalus durissus terrificus, on the uptake of ³H-choline in minces of smooth muscle myenteric plexus from guinea pig ileum. In the concentration range used (0.03-1 µM and up to 10 min of treatment, crotoxin decreased ³H-choline uptake by 50-75% compared to control. This inhibition was time dependent and did not seem to be associated with the disruption of the neuronal membrane, because at least for the first 20 min of tissue exposure to the toxin (up to 1 µM the levels of lactate dehydrogenase (LDH released into the supernatant were similar to those of controls. Higher concentrations of crotoxin or more extensive incubation times with this toxin resulted in elevation of LDH activity detected in the assay supernatant. The inhibitory effect of crotoxin on ³H-choline uptake seems to be associated with its phospholipase activity since the equimolar substitution of Sr2+ for Ca2+ in the incubation medium or the modification of the toxin with p-bromophenacyl bromide substantially decreased this effect. Our results show that crotoxin inhibits ³H-choline uptake with high affinity (EC25 = 10 ± 5 nM. We suggest that this inhibition could explain, at least in part, the blocking effect of crotoxin on neurotransmission.

  12. Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake venom treatment

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    B. P. Tamieti

    2007-01-01

    Full Text Available Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis.

  13. An Evaluation of 3-Rhamnosylquercetin, a Glycosylated Form of Quercetin, against the Myotoxic and Edematogenic Effects of sPLA2 from Crotalus durissus terrificus

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    Daniela de Oliveira Toyama

    2014-01-01

    Full Text Available This paper shows the results of quercitrin effects on the structure and biological activity of secretory phospholipase (sPLA2 from Crotalus durissus terrificus, which is the main toxin involved in the pharmacological effects of this snake venom. According to our mass spectrometry and circular dichroism results, quercetin was able to promote a chemical modification of some amino acid residues and modify the secondary structure of C. d. terrificus sPLA2. Moreover, molecular docking studies showed that quercitrin can establish chemical interactions with some of the crucial amino acid residues involved in the enzymatic activity of the sPLA2, indicating that this flavonoid could also physically impair substrate molecule access to the catalytic site of the toxin. Additionally, in vitro and in vivo assays showed that the quercitrin strongly diminished the catalytic activity of the protein, altered its Vmax and Km values, and presented a more potent inhibition of essential pharmacological activities in the C. d. terrificus sPLA2, such as its myotoxicity and edematogenic effect, in comparison to quercetin. Thus, we concluded that the rhamnose group found in quercitrin is most likely essential to the antivenom activities of this flavonoid against C. d. terrificus sPLA2.

  14. Dosimetria interna de radiofármacos derivados de polipeptídeos antitumorais isolados dos venenos de: Crotalus durissus terrificus e Scorpaena plumieri

    OpenAIRE

    Henrique Martins de Andrade

    2009-01-01

    A identificação de novos agentes terapêuticos e diagnósticos capazes de inibir o crescimento tumoral é essencial para melhoria no prognóstico de pacientes acometidos por tumores malignos (glioma, mama dentre outros). Nesse contexto, produtos naturais (vegetais e animais) constituem-se numa rica fonte de substâncias com potencial antitumoral. Polipeptídeos isolados dos venenos da serpente Crotalus durissus terrificus (Crtx) e do peixe Scorpaena plumieri (SPGP), apresentam atividade...

  15. Mitochondrial dysfunction induced by pancreatic and crotalic (Crotalus durissus terrificus) phospholipases A2 on rabbit proximal tubules suspensions.

    Science.gov (United States)

    Amora, Daniela N; Costa Martins, Alice M; Roeser, Nancy; Senter, Ruth; Ostrowsky, Tiffany; Weinberg, Joel M; Monteiro, Helena S A

    2008-12-15

    In the present study we show that phospholipases A2 isolated from porcine pancreas (PP-PLA2) and Crotalus durissus terrificus snake venom (SV-PLA2) induced dose-dependent increases of LDH release from rabbit proximal tubules in suspension. Both porcine and crotalic PLA(2)s induced increases in non-esterified fatty acid (NEFA) levels (microg of NEFA/mg of tubule protein). It was observed that the NEFA levels in the pellets were higher than in the supernatant for both PLA2, and were dose-dependent for the crotalic PLA2 group. Furthermore, snake venom PLA2 induced a decrease in mitochondrial membrane potential (DeltaPsi(m)) assessed by both JC-1 uptake and safranin O uptake. Porcine PLA2 produced no effects on JC-1 uptake with the highest concentrations and an unexpected increase in the group treated with the lowest concentration. In contrast, the safranin O method revealed decreases of energization with both phospholipases, so it had higher sensitivity to the presence of the increased NEFA levels. Addition of delipidated bovine serum albumin (dBSA) completely reversed the effects induced by phospholipases on DeltaPsi(m) measured with safranin O. Incubation with pancreatic and crotalic phospholipases A2 produced no changes on cell ATP levels. We conclude that the treatment of proximal tubule suspensions with porcine or crotalic phospholipases disturbed membrane integrity as well as mitochondrial function. Specific early NEFA-mediated mitochondrial effects of the phospholipases used in the present study are indicated by the benefit provided by dBSA.

  16. Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768

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    D. P. Netto

    2004-01-01

    Full Text Available Natural (NV and Cobalto60-irradiated (IrV Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0 and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV and the other natural venom (NV. Three antigen doses (venom were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (CBC, total plasma protein, fibrinogen, albumin, and globulin. At the end of the experiment, the animals were challenged with a LD50 for sheep and showed no signs of envenoming. The two groups did not present clinical alterations. Results of the total leukocyte count did not present interaction or time factor effect for both groups, but there was a different action between them, with the NV group presenting more cells than the IrV group. The leukocyte increase to 13,000/ml indicates that slight leukocytosis occurred in the week after the first inoculation in the NV group. There was no statistically significant difference between groups in the absolute count of segmented neutrophils, eosinophils, and lymphocytes but there were statistically significant oscillations in values at the different collecting times. The NV group presented an increase in the absolute neutrophil count after the first inoculation that persisted for 5 weeks. In the IrV group, the increase in neutrophils occurred only in the first week returning to normal in the following weeks. The alterations in the neutrophil count are indicative of systemic inflammatory response related to cytokine release; response was more marked in the NV group, showing its greater toxicity.

  17. Aspectos morfológicos e ultraestruturais de células sanguíneas de Crotalus durissus terrificus

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    Lívia M. Kindlovits

    Full Text Available RESUMO: A avaliação hematológica, de importância comprovada como um meio auxiliar de diagnóstico ao clínico de pequenos animais domésticos, vem se tornando comum em animais selvagens não apenas para a clínica, mas para a avaliação do manejo e como estudo auxiliar para a fisiologia das várias espécies. Tendo em vista o aumento da demanda para a produção de várias drogas de importância farmacêutica, a criação de serpentes peçonhentas vem se tornando comum a ponto destes animais já serem reconhecidos como sendo de produção. O conhecimento do manejo e da clínica destes animais ainda é escasso e a mortalidade é elevada nos criatórios, tornando urgente a ampliação destes. Embora alguns estudos hematológicos já tenham sido realizados em cascavéis (Crotalus durissus os dados analisados ainda são insipientes, notadamente em relação à caracterização das células do sangue e poucos estudos em microscopia eletrônica foram realizados em serpentes. Com o objetivo de caracterizar as células sanguíneas morfologicamente, sob microscopia óptica e ultraestrutural, foram coletadas amostras de sangue de 52 de indivíduos da subespécie Crotalus durissus terrificus para a realização de esfregaços sanguíneos e avaliação ultraestrutural. Concluiu-se que a coloração hematológica de Giemsa permite a avaliação morfológica e a diferenciação das células sanguíneas em serpentes assim como a visualização de hemoparasitos. A avaliação ultraestrutural permite evidenciar as organelas celulares e a diferenciação entre as células, inclusive entre os tipos leucocitários, porém ainda são necessários outros estudos para que seja elucidada a hipótese da existência dos eosinófilos na espécie estudada assim como é necessária melhor caracterização dos grânulos dos azurófilos para que se confirme uma possível diferença entre os monócitos típicos e os azurófilos.

  18. Hematological changes in sheep inoculated with natural and Cobalt{sub 60}-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)

    Energy Technology Data Exchange (ETDEWEB)

    Netto, D.P.; Alfieri, A.A.; Balarim, M.R.S. [Universidade Estadual de Londrina, PR (Brazil). Faculdade de Medicina Veterinaria e Zootecnia. Dept. de Medicina Veterinaria Preventiva]. E-mail: rnetto@uel.br; Chiacchio, S.B.; Bicudo, P.L. [UNESP, Botucatu, SP (Brazil). Faculdade de Medicina Veterinaria e Zootecnia; Nascimento, N. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Supervisao de Radiobiologia

    2004-07-01

    Natural (NV) and Cobalt{sub 60}-irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 m M, p H 3.0) and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV) and the other natural venom (NV). Three antigen doses (venom) were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count, total plasma protein, fibrinogen, albumin, and globulin. At the end of the experiment, the animals were challenged with a LD{sub 50} for sheep and showed no signs of envenoming. The two groups did not present clinical alterations. Results of the total leukocyte count did not present interaction or time factor effect for both groups, but there was a different action between them, with the NV group presenting more cells than the IrV group. The leukocyte increase to 13,000/{mu}l indicates that slight leucocytosis occurred in the week after the first inoculation in the NV group. There was no statistically significant difference between groups in the absolute count of segmented neutrophils, eosinophils, and lymphocytes but there were statistically significant oscillations in values at the different collecting times. The NV group presented an increase in the absolute neutrophil count after the first inoculation that persisted for 5 weeks. In the IrV group, the increase in neutrophils occurred only in the first week returning to normal in the following weeks. The alterations in the neutrophil count are indicative of systemic inflammatory response related to cytokine release; response was more marked in the N V group, showing its greater toxicity. (author)

  19. Crotoxin from Crotalus durissus terrificus is able to down-modulate the acute intestinal inflammation in mice.

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    Caroline de Souza Almeida

    Full Text Available Inflammatory bowel diseases (IBD is the result of dysregulation of mucosal innate and adaptive immune responses. Factors such as genetic, microbial and environmental are involved in the development of these disorders. Accordingly, animal models that mimic human diseases are tools for the understanding the immunological processes of the IBD as well as to evaluate new therapeutic strategies. Crotoxin (CTX is the main component of Crotalus durissus terrificus snake venom and has an immunomodulatory effect. Thus, we aimed to evaluate the modulatory effect of CTX in a murine model of colitis induced by 2,4,6- trinitrobenzene sulfonic acid (TNBS. The CTX was administered intraperitoneally 18 hours after the TNBS intrarectal instillation in BALB/c mice. The CTX administration resulted in decreased weight loss, disease activity index (DAI, macroscopic tissue damage, histopathological score and myeloperoxidase (MPO activity analyzed after 4 days of acute TNBS colitis. Furthermore, the levels of TNF-α, IL-1β and IL-6 were lower in colon tissue homogenates of TNBS-mice that received the CTX when compared with untreated TNBS mice. The analysis of distinct cell populations obtained from the intestinal lamina propria showed that CTX reduced the number of group 3 innate lymphoid cells (ILC3 and Th17 population; CTX decreased IL-17 secretion but did not alter the frequency of CD4+Tbet+ T cells induced by TNBS instillation in mice. In contrast, increased CD4+FoxP3+ cell population as well as secretion of TGF-β, prostaglandin E2 (PGE2 and lipoxin A4 (LXA4 was observed in TNBS-colitis mice treated with CTX compared with untreated TNBS-colitis mice. In conclusion, the CTX is able to modulate the intestinal acute inflammatory response induced by TNBS, resulting in the improvement of clinical status of the mice. This effect of CTX is complex and involves the suppression of the pro-inflammatory environment elicited by intrarectal instillation of TNBS due to the

  20. Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins

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    CARLOS H.M. OLIVEIRA

    Full Text Available ABSTRACT The enzyme inhibition by natural and/ or low-cost compounds may represent a valuable adjunct to traditional serotherapy performed in cases of snakebite, mainly with a view to mitigate the local effects of envenoming. The objective of this study was to evaluate possible interactions between vitamins and enzymes that comprise Bothrops atrox and Crotalus durissus terrificus venoms, in vitro. Proteolysis inhibition assays (substrates: azocasein, collagen, gelatin and fibrinogen, hemolysis, coagulation, hemagglutination were carried out using different proportions of vitamins in face of to inhibit minimum effective dose of each venom. The vitamins were responsible for reducing 100% of breaking azocasein by C.d.t. venom, thrombolysis induced by B. atrox and fibrinogenolysis induced by both venoms. It is suggested the presence of interactions between vitamin and the active site of enzymes, for example the interactions between hydrophobic regions present in the enzymes and vitamin E, as well as the inhibitions exercised by antioxidant mechanism.

  1. Pulmonary, microbiological and hematological changes in Crotalus durissus terrificus (Serpentes, Viperidae parasitized by nematodes of the genus Rhabdias (Nematoda, Rhabdiasidae Alterações Pulmonares, microbiológicas e hematológicas em Crotalus durissus terrificus (Serpentes, Viperidae parasitadas pelos nematódeos do gênero Rhabdias (Nematoda, Rhabdiasidae

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    K.R. Santos

    2008-06-01

    Full Text Available This study reported the pulmonary, microbiological, and hematological alterations in Crotalus durissus terrificus parasitized by nematodes of the genus Rhabdias. Histological, microbiological, and hematological analysis were performed on parasitized (n=6 and non-parasitized (n=6 snakes. Granulocytic and mononuclear cell infiltrates in the pulmonary parenchyma and epithelium were also observed during the histological analysis of parasitized snakes. Microbiological analysis of parasitized animals revealed the following Gram-negative bacteria: Citrobacter divergens, Burkholderia cepacia, Stenotrophomonas maltophilia, Proteus vulgaris, Enterobacter sakazakii, Enterobacter ammnigenus, Pseudomonas aeruginosa, Pantoea sp. and Providencia rettgeri. In non-parasitized snakes, the following species were identified: B. cepacia, Pseudomonas fluorescens, and Acinetobacter baumanii. Increased levels of plasmatic protein, decreased lymphocyte counts, and normal red blood cell values were observed in parasitized animals.Este trabalho relata as alterações pulmonares, microbiológicas e hematológicas em Crotalus durissus terrificus parasitadas pelo nematódeo do gênero Rhabdias. As análises histológicas, microbiológicas e hematológicas foram realizadas em serpentes parasitadas (n=6 e não parasitadas (n=6. Foram observados infiltrados de células granulocíticas e mononucleares no parênquima pulmonar durante a análise histopatológica das serpentes parasitadas. A análise microbiológica revelou as seguintes bactérias Gram-negativas; Citrobacter divergens, Burkholderia cepacia, Stenotrophomonas maltophilia, Proteus vulgaris, Enterobacter sakazakii, Enterobacter ammnigenus, Pseudomonas aeruginosa, Pantoea sp. e Providencia rettgeri. Nas serpentes não parasitadas foram identificadas: B. cepacia, Pseudomonas fluorescens e Acinetobacter baumanii. Nos animais parasitados observaram-se: aumento da concentração de proteína plasmática, diminuição da

  2. Humoral response and neutralization capacity of sheep serum inoculated with natural and Cobalt 60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)

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    Netto, D.P.; Alfieri, A.A. [Universidade Estadual de Londrina, PR (Brazil). Centro de Ciencias Agrarias, Dept. de Medicina Veterinaria Preventiva; Chiacchio, S.B.; Bicudo, P.L. [UNESP, SP (Brazil). Faculdade de Medicina Veterinaria e Zootecnia; Nascimento, N. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Supervisao de Radiobiologia]. E-mail: rnetto@uel.br

    2002-07-01

    The aim of this work was to investigate antigen irradiation on crotalic antivenom and the capacity of sheep as serum producers. Twelve sheep in two groups of six were inoculated with Crotalus durissus terrificus venom. One group was inoculated with natural venom (N V) and the other with Cobalt 60 gamma-irradiated venom (Ir V). Three antigen doses were given to the animals at monthly intervals for immunization. The toxic activity of the venom was assessed by LD 50 determination in mice. Blood samples were collected weekly analyses of serum neutralization capacity and potency. At the end of the experiment, the animals were challenged with a LD 50 for sheep showed no signs of envenoming. These results showed that toxicity of the irradiated venom was 4.4 times less than the natural venom. The sera from the irradiated group neutralized LD 50 14.6 times, and the sera from the natural group 4.4 times. Sera from the irradiated group were five times more potent. The two groups did not present clinical alterations. The results of this study show the potential for using sheep in crotalic antivenom production. The use of irradiated venom in sheep immunization induces a powerful and lasting humoral immune response shown by both the in vitro neutralization and potency tests and by the indirect ELISA antibody level detection technique. (author)

  3. Characterization of ribonucleic acids from the venom glands of Crotalus durissus terrificus (Ophidia, Reptilia) after manual extraction of the venom. Studies on template activity and base composition

    Science.gov (United States)

    De Lucca, F. L.; Imaizumi, M. T.; Haddad, A.

    1974-01-01

    RNA synthesis in the venom glands of Crotalus durissus terrificus was stimulated by the manual extraction of the venom (milking). RNA was extracted from venom glands activated by milking and fractionated by centrifugation through sucrose density gradients. Template activity for protein synthesis and base composition of the RNA fractions were studied. RNA fractions that sediment between 18S and 4S had the highest template activity. The base composition analysis indicated that the 28S and 18S rRNA have a C+G content of 65.4 and 58% respectively. The `melting' temperature (Tm) of DNA in 0.15m-NaCl–0.015m-trisodium citrate, pH7.0, was 85°C, corresponding to a C+G content of 38%. The base ratio of the RNA fractions that showed a high template activity was intermediate between that of rRNA and homologous DNA. The possible role of these fractions in the synthesis of the two main toxins (crotoxin and crotamine) of the South American rattlesnake's venom is discussed. ImagesPLATE 1 PMID:4463939

  4. Rabdomióilise secundária a acidente ofídico crotálico (Crotalus durissus terrificus

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    Renato Almeida Magalhães

    1986-08-01

    Full Text Available Relatam-se dois casos de rabdomiólise secundária a envenenamento produzido por Crotalus durissus terrificus. O diagnóstico da rabdomiólise baseou-se na mialgia intensa e generalizada apresentada pelos pacientes e na constatação de níveis séricos elevados de CPK, TGO e DHL. A confirmação do diagnóstico foi obtida no caso n.° 2 pela detecção de mioglobina no soro através de imunoeletroforese contra soro antimioglobina humana e por biópsia muscular. Esse paciente desenvolveu também, como complicação do envenenamento ofídico, quadro clínico e laboratorial de insuficiência renal aguda. Essa complicação foi atribuída à ação nefrotóxica e hemolítica do veneno crotálico e à hipotensão arterial apresentada pelo paciente, não se afastando a possibilidade de que a rabdomiólise tenha sido um fator contribuinte para a sua instalação. Foram constatadas hipocalcemla, hiperuricemia e hiperfosfatemia grave na fase oligúria da insuficiência renal aguda, alterações peculiares quando essa condição está associada à rabdomiólise.

  5. Effects of N-acetyl-L-cysteine on redox status and markers of renal function in mice inoculated with Bothrops jararaca and Crotalus durissus terrificus venoms.

    Science.gov (United States)

    Barone, Juliana Marton; Frezzatti, Rodrigo; Silveira, Paulo Flavio

    2014-03-01

    Renal dysfunction is an important aggravating factor in accidents caused by Crotalus durissus terrificus (Cdt) and Bothrops jararaca (Bj) bites. N-acetyl-l-cysteine (NAC) is well known as a nephroprotective antioxidant with low toxicity. The present study investigated the effects of NAC on redox status and markers of renal function in mice that received vehicle (controls) or venoms (v) of Cdt and Bj. In controls NAC promoted hypercreatinemia, hypouremia, hyperosmolality with decreased urea in urine, hyperproteinuria, decreased protein and increased dipeptidyl peptidase IV (DPPIV) in membrane-bound fraction (MF) from renal cortex (RC) and medulla (RM). NAC ameliorated or normalized altered creatinuria, proteinemia and aminopeptidase (AP) acid in MF, AP basic (APB) in soluble fraction (SF), and neutral AP in SF and MF from RC and RM in vBj envenomation. NAC ameliorated or normalized altered neutral AP in SF from RC and RM, and DPPIV and protein in MF from RC in vCdt envenomation. NAC ameliorated or restored renal redox status respectively in vCdt and vBj, and normalized uricemia in both envenomations. These data are promising perspectives that recommend the clinical evaluation of NAC as potential coadjuvant in the anti venom serotherapy for accidents with these snake's genera.

  6. Sulfated polysaccharide extracted of the green algae Caulerpa racemosa increase the enzymatic activity and paw edema induced by sPLA2 from Crotalus durissus terrificus venom

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    Camila L. Pires

    2013-08-01

    Full Text Available Sulfated polysaccharides derived from seaweed have shown great potential for use in the development of new drugs. In this study, we observed that a low-molecular-weight sulfated polysaccharide from Caulerpa racemosa, termed CrSP, could interact with secretory phospholipase A2 (sPLA2 isolated from Crotalus durissus terrificus venom. When native sPLA2 (14 kDa was incubated with CrSP, they formed a molecular complex (sPLA2:CrSP with a molecular mass of 32 kDa, approximately. Size exclusion chromatography experiments suggested that CrSP formed a stable complex with sPLA2. We belived that sPLA2 and SPCr are involved an ionic interaction between negatively charged CrSP and the positively charged basic amino acid residues of sPLA2, because this interaction induced significant changes in sPLA2 enzymatic and pharmacological activities. CrSP caused a significant increase in sPLA2 enzymatic and bactericidal activity and increased its edematogenic effect. A pharmacological assay showed that the myotoxic activity of sPLA2:CrSP is unrelated to its enzymatic activity and that sPLA2:CrSP may have a practical application as a natural antibacterial agent for use in humans and commercially raised animals.

  7. Phospholipase A2 isolated from the venom of Crotalus durissus terrificus inactivates dengue virus and other enveloped viruses by disrupting the viral envelope.

    Science.gov (United States)

    Muller, Vanessa Danielle; Soares, Ricardo Oliveira; dos Santos, Nilton Nascimento; Trabuco, Amanda Cristina; Cintra, Adelia Cristina; Figueiredo, Luiz Tadeu; Caliri, Antonio; Sampaio, Suely Vilela; Aquino, Victor Hugo

    2014-01-01

    The Flaviviridae family includes several virus pathogens associated with human diseases worldwide. Within this family, Dengue virus is the most serious threat to public health, especially in tropical and sub-tropical regions of the world. Currently, there are no vaccines or specific antiviral drugs against Dengue virus or against most of the viruses of this family. Therefore, the development of vaccines and the discovery of therapeutic compounds against the medically most important flaviviruses remain a global public health priority. We previously showed that phospholipase A2 isolated from the venom of Crotalus durissus terrificus was able to inhibit Dengue virus and Yellow fever virus infection in Vero cells. Here, we present evidence that phospholipase A2 has a direct effect on Dengue virus particles, inducing a partial exposure of genomic RNA, which strongly suggests inhibition via the cleavage of glycerophospholipids at the virus lipid bilayer envelope. This cleavage might induce a disruption of the lipid bilayer that causes a destabilization of the E proteins on the virus surface, resulting in inactivation. We show by computational analysis that phospholipase A2 might gain access to the Dengue virus lipid bilayer through the pores found on each of the twenty 3-fold vertices of the E protein shell on the virus surface. In addition, phospholipase A2 is able to inactivate other enveloped viruses, highlighting its potential as a natural product lead for developing broad-spectrum antiviral drugs.

  8. Phospholipase A2 isolated from the venom of Crotalus durissus terrificus inactivates dengue virus and other enveloped viruses by disrupting the viral envelope.

    Directory of Open Access Journals (Sweden)

    Vanessa Danielle Muller

    Full Text Available The Flaviviridae family includes several virus pathogens associated with human diseases worldwide. Within this family, Dengue virus is the most serious threat to public health, especially in tropical and sub-tropical regions of the world. Currently, there are no vaccines or specific antiviral drugs against Dengue virus or against most of the viruses of this family. Therefore, the development of vaccines and the discovery of therapeutic compounds against the medically most important flaviviruses remain a global public health priority. We previously showed that phospholipase A2 isolated from the venom of Crotalus durissus terrificus was able to inhibit Dengue virus and Yellow fever virus infection in Vero cells. Here, we present evidence that phospholipase A2 has a direct effect on Dengue virus particles, inducing a partial exposure of genomic RNA, which strongly suggests inhibition via the cleavage of glycerophospholipids at the virus lipid bilayer envelope. This cleavage might induce a disruption of the lipid bilayer that causes a destabilization of the E proteins on the virus surface, resulting in inactivation. We show by computational analysis that phospholipase A2 might gain access to the Dengue virus lipid bilayer through the pores found on each of the twenty 3-fold vertices of the E protein shell on the virus surface. In addition, phospholipase A2 is able to inactivate other enveloped viruses, highlighting its potential as a natural product lead for developing broad-spectrum antiviral drugs.

  9. Evidence for a respiratory component, similar to mammalian respiratory sinus arrhythmia, in the heart rate variability signal from the rattlesnake, Crotalus durissus terrificus.

    Science.gov (United States)

    Campbell, Hamish A; Leite, Cleo A C; Wang, Tobias; Skals, Marianne; Abe, Augusto S; Egginton, Stuart; Rantin, F Tadeu; Bishop, Charles M; Taylor, Edwin W

    2006-07-01

    Autonomic control of heart rate variability and the central location of vagal preganglionic neurones (VPN) were examined in the rattlesnake (Crotalus durissus terrificus), in order to determine whether respiratory sinus arrhythmia (RSA) occurred in a similar manner to that described for mammals. Resting ECG signals were recorded in undisturbed snakes using miniature datalogging devices, and the presence of oscillations in heart rate (fh) was assessed by power spectral analysis (PSA). This mathematical technique provides a graphical output that enables the estimation of cardiac autonomic control by measuring periodic changes in the heart beat interval. At fh above 19 min(-1) spectra were mainly characterised by low frequency components, reflecting mainly adrenergic tonus on the heart. By contrast, at fh below 19 min(-1) spectra typically contained high frequency components, demonstrated to be cholinergic in origin. Snakes with a fh >19 min(-1) may therefore have insufficient cholinergic tonus and/or too high an adrenergic tonus acting upon the heart for respiratory sinus arrhythmia (RSA) to develop. A parallel study monitored fh simultaneously with the intraperitoneal pressures associated with lung inflation. Snakes with a fhrate (fv). Adrenergic blockade by propranolol infusion increased the variability of the ventilation cycle, and the oscillatory component of the fh spectrum broadened accordingly. Infusion of atropine to effect cholinergic blockade abolished this HF component, confirming a role for vagal control of the heart in matching fh and fv in the rattlesnake. A neuroanatomical study of the brainstem revealed two locations for vagal preganglionic neurones (VPN). This is consistent with the suggestion that generation of ventilatory components in the heart rate variability (HRV) signal are dependent on spatially distinct loci for cardiac VPN. Therefore, this study has demonstrated the presence of RSA in the HRV signal and a dual location for VPN in the

  10. Comparación entre las técnicas de neutralización en ratón y E.L.I.S.A para determinar la potencia del anti-veneno crotalus durissus terrificus

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    María Cristina Forero CH.

    1984-12-01

    Full Text Available Las técnicas de neutralización en ratón y ELISA se compararon como métodos para determinar la potencia del antiveneno Crotalus durissus terrificus. lnformes en la literatura(3, (12, (13, sugerían la posibilidad de cambiar la técnica in vivo muy costosa por la técnica in vitro. Se utilizaron antivenenos procesados (Gamma-globulinas purificadas y no procesados. No se encontró correlación entre los títulos de neutralización y las lecturas de absorvancia obtenidas por ELISA para ninguna de las muestras. La falta de correlación se debe probablemente a que las técnicas miden dos poblaciones de anticuerpos diferentes. Mientras que con ELlSA se miden anticuerpos contra todos los antígenos del veneno, con la técnica de neutralización solo se miden anticuerpos contra la fracción letal. Se sugiere verificar nuevamente la correlación entre las dos técnicas usando como antígeno para ELISA la neurotoxina purificada. No se recomienda, en contra a lo afirmado por otros autores (3, el empleo de la técnica de ELlSA usando veneno total como antígeno, para determinar la potencia del antiveneno Crotalus durissus terrificus.

  11. Resposta imune e capacidade de neutralização de anticorpos produzidos em ovinos jovens imunizados com veneno de Crotalus durissus terrificus nativo e irradiado com Cobalto 60

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    Rui Seabra Ferreira Junior

    2009-06-01

    Full Text Available A técnica de Elisa foi utilizada para avaliar e comparar a resposta imune humoral de ovinos jovens para a produção de soro anticrotálico. Durante o processo de soroprodução, foi realizada a avaliação clínica dos animais. A capacidade de neutralização do soro produzido a partir de veneno de serpente Crotalus durissus terrificus, nativo (VN e irradiado (VIr com Cobalto-60 foi verificada por meio de desafios in vitro. Um grupo de seis animais recebeu veneno nativo, o segundo grupo recebeu veneno irradiado e o terceiro grupo foi o controle. Os animais receberam seis imunizações durante 84 dias com intervalo de 14 dias. Houve diferença significativa (p<5% no teste de ELISA do perfil de anticorpos produzidos pelos grupos experimentais (VN < VIr. O grupo imunizado com veneno irradiado apresentou perfil de anticorpos maior que o grupo imunizado com veneno nativo. A capacidade de neutralização do soro produzido a partir do VIr foi cinco vezes maior quando comparado ao soro produzido com VN. Estes resultados justificam o uso da radiação gama na destoxicação do veneno de Crotalus durissus terrificus como alternativa na produção de antiveneno.

  12. Rabbit IgG antibodies against Phospholipase A2 from Crotalus durissus terrificus neutralize the lethal activity of the venom Los anticuerpos IgG de conejos anti-fosfolipasa A2 de Crotalus durissus terrificus neutralizan la actividad letal del veneno

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    Juan P. Rodríguez

    2006-12-01

    Full Text Available Crotalus durissus terrificus (C.d.t. (South American rattlesnake venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2 and a non toxic acidic protein, crotapotin. We have produced and investigated the ability of IgG antibodies raised in rabbits against PLA2 to neutralize the lethality of the whole venom. PLA2 was isolated by gel filtration chromatography (Sephadex G-75. Specific antibodies were obtained by subcutaneous and intramuscular inoculation of PLA2 (700 µg with Freund adjuvant. Groups of six mice (20 + 2 g were inoculated with 0.5 ml i.p. of C. d. t. venom (4 µg or a mixture of venom that had been preincubated with the desired volume of IgG antibodies. Mortality, recorded 24 and 48 h after inoculation, showed that IgG anti-PLA2 were more effective than anticrotalic serum in neutralizing the lethal activity. These results demonstrate that it could be possible to obtain an anti-venom made by specific antibodies with a high level of protection against the lethal component of C.d.t. venom, and/or the inclusion of these antibodies as a supplement in heterologous anti-venoms.El veneno de Crotalus durissus terrificus (C.d.t. (Cascabel de Sud América posee actividad miotóxica y neurotóxica, actividades que también exhibe el complejo crotoxina, principal componente tóxico de este veneno. El complejo crotoxina está constituido por una fosfolipasa A2 básica (PLA2 y una proteína acídica no tóxica, el crotapotín. En este trabajo se estudió la capacidad neutralizante de anticuerpos IgG anti-PLA2 sobre la letalidad inducida por el veneno entero. El antígeno PLA2, fue aislado por cromatografía de filtración en gel (Sephadex G-75. Se inocularon conejos machos por vía subcutánea e intramuscular, con 700 µg de PLA2 y adyuvante para la obtención de anticuerpos específicos. La capacidad neutralizante del

  13. The comparison between the humoral response and the neutralizing capacity of sheep serum inoculated with natural venom and Co{sup 60} irradiated venom from Crotalus durissus terrificus (Laurenti, 1768)

    Energy Technology Data Exchange (ETDEWEB)

    Netto, D.P. [Universidade Estadual de Londrina, PR (Brazil). Centro de Ciencias Agrarias. Dept. de Medicina Veterinaria Preventiva]. E-mail: mnetto@uel.br

    2000-07-01

    Crotalus durissus terrificus venom was irradiated with Co{sup 60} to investigate the effects of antigen-irradiation on antivenom production in sheep. Twelve sheep were divided in two groups of 6. One group received irradiated, while the other received natural venom. Three doses of antigen were given at monthly intervals. The toxic activity of the venom was assessed by LD{sub 50} in mice. Weekly blood samples were obtained to evaluate anti-crotalic serum titers by indirect ELISA, neutralization capacity, and serum potency. A complete blood count, plasma protein and fibrinogen concentration, and serum albumin and globulin were also determined. At end of the experiment, the animals were challenged with ovine LD{sub 50}, without clinical abnormalities. (author)

  14. Quadros clínico-patológicos do envenenamento ofídico por Crotalus durissus terrificus e Bothrops spp. em animais de produção

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    Carlos Hubinger Tokarnia

    2014-04-01

    Full Text Available Foi realizada uma revisão dos quadros clínico-patológicos causados pelos venenos de Crotalus durissus terrificus e Bothrops spp. em bovinos, búfalos, ovinos equinos e suínos. Foram compilados os dados obtidos pela experimentação em animais de produção encontrados na literatura e os obtidos através de experimentação realizada por nossa equipe. Também foram revisados os casos naturais de envenenamento ofídico comunicados. Em dois Quadros foram lançados os mais importantes dados dessas revisões, que revelou diversos aspectos interessantes: 1 em nossos experimentos, o veneno de Crotalus durissus terrificus, quando injetado por via subcutânea em cavalos, causou um edema acentuado no local da aplicação, ao contrário do que tem sido observado em todas as outras espécies animais, aspecto não relatado na literatura; 2 em nossos experimentos, o veneno de diversas espécies de Bothrops, quando injetado por via subcutânea em bovinos, ovinos e equinos, não causou edema como em geral é relatado na literatura, e sim hemorragias subcutâneas acentuadas no local da aplicação. Nos casos não fatais este sangue era reabsorvido em poucos dias sem deixar sequelas. Exceção foi a reação ao veneno de Bothrops jararacussu, que causou edema nos ovinos experimentais, e tumefação acentuada que resultou em fístula com eliminação de líquido seroso nos equinos experimentais. O objetivo do presente estudo visa contribuir para o aperfeiçoamento do diagnóstico de acidentes ofídicos em animais de produção.

  15. Internal dosimetry of radiopharmaceuticals derived of antitumor polypeptide isolated from venoms: Crotalus durissus terrifucus and Scorpaena plumieri;Dosimetria interna de radiofarmacos derivados de polipeptideos antitumorais isolados dos venenos de: Crotalus durissus terrificus e Scorpaena plumieri

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Henrique Martins de

    2009-07-01

    The identification of new diagnostic and therapeutic agents capable of inhibiting tumor growth is essential for improving the prognosis of patients suffering from malignant tumors (glioma, breast and others). In this context, natural products (plants and animals) are a rich source of substances with potential antitumor. Despite knowledge of the etiology and pathology of tumors little progress has been observed in the area of diagnosis. Molecules of snake venoms have been shown to play an important role not only in the survival and proliferation of tumor cells but also in the process of tumor cell adhesion, migration and angiogenesis. Polypeptides isolated from the venom of the snake, Crotalus durissus terrificus, Crtx, and Scorpaena plumieri fish, SPGP, have antitumor activity against malignant tumors. It was shown that similar radio iodines Crtx and SPGP, {sup 125}I-Crtx and {sup 125}I-SPGP, can interact specifically with malignant tumors and induce cell death. Prototype-based radiopharmaceuticals Crtx and SPGP containing radioiodine 1311 were able to produce diagnostic images to accumulate specifically in the tumor site. The present study aimed at evaluating the potential radiological safety and diagnostic/therapeutic efficacy of {sup 131}I-Crtx {sup l31}I-SPGP and (evaluated from the biokinetic data in mice bearing Ehrlich tumor) were treated by the MIRD formalism to carry out internal dosimetry studies. Absorbed doses due to the uptake of {sup 131}I-Crtx and {sup 131}I-SPGP were determined in various organs of mice and implanted into the tumor. The results obtained for the animal model were extrapolated to humans by assuming a similar concentration ratio among the various tissues between mice and humans. In extrapolation, we used the masses of human organs of the phantom of Cristy/Eckerman. Both radiation penetrating and non penetrating of {sup 131}I on the tissue were considered in dose calculations. The absorbed dose in the bone marrow due to the

  16. Comparison of humoral immune response, neutralization capacity of anticrotalic serum in young ovines, clinical and weight evaluation between animals inoculated with Crotalus durissus terrificus venom, natural or Cobalt-60-irradiated

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira Junior, R.S. E-mail: rseabra@cevap.org.br

    2005-07-01

    The Elisa technique was used to evaluate and compare the humoral immune response of young ovine to anticrotalic serum production. During serum production, the clinical and weight evaluation of the animals was performed. The parameters utilized were complete blood count, and dosage of urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin. The animals weight was verified fortnightly during the experiment. The neutralization capacity of the serum produced from the snake Crotalus durissus terrificus natural (NV) and Cobalt-60-irradiated venom (IrV) was evaluated by in vitro challenges. One group of six animals received natural venom, the second group received irradiated venom, and the third was the control group. The animals received six immunizations during 84 days with an interval of 14 days. There was a significant difference (p<5%) in the ELISA test for the profile of the antibodies produced by the experimental groups (NV

  17. Effect of Mikania glomerata (Asteraceae) leaf extract combined with anti-venom serum on experimental Crotalus durissus (Squamata: Viperidae) envenomation in rats

    OpenAIRE

    Rafael Stuani Floriano; Rosa Maria Barilli Nogueira; Michiko Sakate; Cecília Braga Laposy; Yudney Pereira da Motta; Fabíola Sangiorgio; Heloísa Costa David; João Marcelo Nabas

    2009-01-01

    Crotalic envenomation represents the highest number of deaths when compared to other snakebite envenomations of medical interest. Crotalic venom has important characteristics such as neurotoxicity, myotoxicity, nephrotoxicity, and clotting and hemolytic action. We evaluated the clinical and laboratory aspects of Crotalus durissus terrificus experimental envenomation in Wistar rats treated with antivenom and the aqueous extract of the plant Mikania glomerata. The animals were divided into thre...

  18. Snake venomics of the Central American rattlesnake Crotalus simus and the South American Crotalus durissus complex points to neurotoxicity as an adaptive paedomorphic trend along Crotalus dispersal in South America.

    Science.gov (United States)

    Calvete, Juan J; Sanz, Libia; Cid, Pedro; de la Torre, Pilar; Flores-Díaz, Marietta; Dos Santos, M Cristina; Borges, Adolfo; Bremo, Adolfo; Angulo, Yamileth; Lomonte, Bruno; Alape-Girón, Alberto; Gutiérrez, José María

    2010-01-01

    We report a comparative venomic and antivenomic characterization of the venoms of newborn and adult specimens of the Central American rattlesnake, Crotalus simus, and of the subspecies cumanensis, durissus, ruruima, and terrificus of South American Crotalus durissus. Neonate and adult C. simus share about 50% of their venom proteome. The venom proteome of 6-week-old C. simus is predominantly made of the neurotoxic heterodimeric phospholipase A(2) (PLA(2) crotoxin) (55.9%) and serine proteinases (36%), whereas snake venom Zn(2+)-metalloproteinases (SVMPs), exclusively of class PIII, represent only 2% of the total venom proteins. In marked contrast, venom from adult C. simus comprises toxins from 7 protein families. A large proportion (71.7%) of these toxins are SVMPs, two-thirds of which belong to the PIII class. These toxin profiles correlate well with the overall biochemical and pharmacological features of venoms from adult (hemorrhagic) and newborn (neurotoxic) C. simus specimens. The venoms of the South American Crotalus subspecies belong to one of two distinct phenotypes. C. d. cumanensis exhibits high levels of SVMPs and low lethal potency (LD(50)), whereas C. d. subspecies terrificus, ruruima, and durissus have low SVMP activity and high neurotoxicity to mice. Their overall toxin compositions explain the outcome of envenomation by these species. Further, in all C. simus and C. durissus venoms, the concentration of neurotoxins (crotoxin and crotamine) is directly related with lethal activity, whereas lethality and metalloproteinase activity show an inverse relationship. The similar venom toxin profiles of newborn C. simus and adult C. durissus terrificus, ruruima, and durissus subspecies strongly suggests that the South American taxa have retained juvenile venom characteristics in the adult form (paedomorphism) along their North-South stepping-stone dispersal. The driving force behind paedomorphism is often competition or predation pressure. The increased

  19. Effect of Mikania glomerata (Asteraceae) leaf extract combined with anti-venom serum on experimental Crotalus durissus (Squamata: Viperidae) envenomation in rats.

    Science.gov (United States)

    Floriano, Rafael Stuani; Nogueira, Rosa Maria Barilli; Sakate, Michiko; Laposy, Cecília Braga; da Motta, Yudney Pereira; Sangiorgio, Fabíola; David, Heloísa Costa; Nabas, João Marcelo

    2009-12-01

    Crotalic envenomation represents the highest number of deaths when compared to other snakebite envenomations of medical interest. Crotalic venom has important characteristics such as neurotoxicity, myotoxicity, nephrotoxicity, and clotting and hemolytic action. We evaluated the clinical and laboratory aspects of Crotalus durissus terrificus experimental envenomation in Wistar rats treated with antivenom and the aqueous extract of the plant Mikania glomerata. The animals were divided into three groups: Group C (control); Group VS-venom and antivenom; Group VSM-venom, antivenom and aqueous extract of M. glomerata. Crotalic poison caused clinical and laboratory alterations in Wistar mice. Significant clinical alterations were: temperature decrease, edema in the venom inoculated member, sedation and a locomotion decrease in groups VS and VSM when compared with group C. A faster recovery from sedation was observed only for animals of group VSM when compared to VS. There was an increase in the number of leukocytes, neutrophils and creatine kinase in the VS and VSM groups, compared to group C. Wistar rats showed a high resistance to crotalic venom. Additional studies with different doses, time of treatment, different administration methods and histopathological and immunological studies are necessary to understand the action of M. glomerata in crotalic accidents.

  20. Projeto "Viver em Cascavel": análise do fluxo de informações Proyecto "Vivir en Cascavel": análisis del flujo de informaciones Project "To live in Cascavel": analysis of the information flow

    Directory of Open Access Journals (Sweden)

    Nelsi Salete Tonini Paiva

    2002-07-01

    Full Text Available O presente estudo estimou e analisou o tempo (em dias do fluxo das Declarações de Nascidos Vivos (DNV de risco atendidos no Projeto "Viver em Cascavel"- Paraná, 1996 a 1998, segundo os espaços organizativos (hospital, vigilância epidemiológica, unidade básica de saúde. Foram analisadas 303 declarações. Para análise do tempo de fluxo da DNV, foram construídos intervalos de 95% de confiança para os tempos médios populacionais (em dias. Os resultados apontam que a DNV demorou para fluir do hospital até o momento de realização da visita domiciliária, de 25 a 30 dias. A atenção ao recém nascido de risco deve acontecer nos prazos mais breves possíveis. Nesse sentido, o tempo de fluxo da DNV encontrado neste estudo, do nascimento à visita domiciliária, pode comprometer um dos objetivos do Projeto em questão, qual seja, a redução da morbimortalidade infantil no município.El presente estudio estimó y analizó el tiempo (en días de flujo de los certificados de nacimiento de niños con riesgo, atendidos en el Proyecto "Vivir en Cascavel" - Paraná, 1996 hasta 1998, según los espacios organizacionales (hospital, vigilancia epidemiológica, unidad básica de salud. Fueron analizados 303 certificados. Para el análisis del tiempo de flujo del certificado fueron construidos intervalos de 95% de confianza para los tiempos medios poblacionales (en días. Los resultados muestran que el tiempo promedio de flujo es largo, entre 25 y 30 días. La atención al recién nacido de riesgo debe acontecer en plazos lo más breves posibles y, en este sentido, el tiempo de flujo del certificado, desde el nacimiento hasta la visita domiciliaria, puede estar comprometiendo uno de los objetivos del proyecto analizado, o sea, la reducción de la morbi-mortalidad infantil en el municipio.This study estimated and evaluated the time period (in days concerning the flow of Live Birth Declarations (DNV for high-risk newborns assisted by the Project

  1. Avaliação do Centro de Atenção Psicossocial Infantil de Cascavel - PR Evaluation of the Child Psychosocial Care Center of Cascavel - PR

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    Ana Silvia Scandolara

    2009-12-01

    Full Text Available Estudo relacionado à avaliação de serviços em saúde mental, neste caso, o Centro de Atenção Psicossocial Infantil - CAPSi, do município de Cascavel-PR, serviço substitutivo implementado a partir da transformação da assistência psiquiátrica no Brasil. Objetivou avaliar qualitativamente o CAPSi. Os participantes foram 10 profissionais de saúde que atuam no CAPSi, sendo que os dados foram analisados sob a técnica do Discurso do Sujeito Coletivo (DSC (Lefèvre & Lefèvre, 2003. Como resultado, construíram-se 06 DSC e destacaram-se 12 temas geradores de significações: bom atendimento, estrutura física, recursos humanos, rede de serviços, acesso, referência, organização do serviço, família, atende as políticas de saúde mental, demanda, capacitação, redefinição de serviços, qualidade do serviço, papel do CAPSi e rotatividade de recursos humanos. Com base nos DSC o atendimento prestado no CAPSi é de boa qualidade, porém existem dificuldades de estrutura administrativa.This study is related to the evaluation of mental health services, in this case, the Child Psychosocial Care Center - (Centro de Atenção Psicossocial Infantil - CAPSi, in the city of Cascavel-PR, that is a substitute service implemented from the transformation of the psychiatric assistance in Brazil. It objectified to evaluate CAPSi qualitatively. The participants have been 10 health professionals who act in CAPSi, and the data has been analyzed under the technique of the General Subject Speech ( Discurso do Sujeito Coletivo - DSC (Lefèvre & Lefèvre, 2003. As result, 06 DSC have been constructed and 12 meaning-generating themes have been distinguished: good attendance, physical structure, human resources, net of services, access, reference, organization of the service, family, respect for the mental health policies, demand, qualification, redefinition of services, quality of the service, role of the CAPSi and rotation of human resources. Based on the

  2. Effect of Mikania glomerata (Asteraceae leaf extract combined with anti-venom serum on experimental Crotalus durissus (Squamata: Viperidae envenomation in rats

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    Rafael Stuani Floriano

    2009-12-01

    Full Text Available Crotalic envenomation represents the highest number of deaths when compared to other snakebite envenomations of medical interest. Crotalic venom has important characteristics such as neurotoxicity, myotoxicity, nephrotoxicity, and clotting and hemolytic action. We evaluated the clinical and laboratory aspects of Crotalus durissus terrificus experimental envenomation in Wistar rats treated with antivenom and the aqueous extract of the plant Mikania glomerata. The animals were divided into three groups: Group C (control; Group VS-venom and antivenom; Group VSM-venom, antivenom and aqueous extract of M. glomerata. Crotalic poison caused clinical and laboratory alterations in Wistar mice. Significant linical alterations were: temperature decrease, edema in the venom inoculated member, sedation and a locomotion decrease in groups VS and VSM when compared with group C. A faster recovery from sedation was observed only for animals of group VSM when compared to VS. There was an increase in the number of leukocytes, neutrophils and creatine kinase in the VS and VSM groups, compared to group C. Wistar rats showed a high resistance to crotalic venom. Additional studies with different doses, time of treatment, different administration methods and histopathological and immunological studies are necessary to understand the action of M. glomerata in crotalic accidents. Rev. Biol. Trop. 57 (4: 929-937. Epub 2009 December 01.El envenamiento crotálico representa el número más alto de muertes cuando es comparado con envenenamientos por mordeduras de otras serpientes de interés médico. El veneno crotálico tiene importantes características de acción neurotóxica, miotoxicidad, nefrotoxicidad, coagulación y acción hemolítica. Este trabajo evaluó los aspectos clínicos y de laboratorio del envenenamiento experimental con el veneno de la serpiente Crotalus durissus terrificus en las ratas Wistar tratadas con suero antiofídico y extracto acuoso de M

  3. Produção e caracterização parcial de nanocorpos ativos contra Crotoxina: uma neurotoxina da serpente Crotalus durissus terrificus.

    OpenAIRE

    Luiz, Marcos Barros

    2014-01-01

    Dissertação apresentada ao Programa de Pós- Graduação: Mestrado em Biologia Experimental (PGBIOEXP) da Fundação Universidade Federal de Rondônia (UNIR) como requisito final para a obtenção do título de Mestre em Biologia Experimental. Orientadora: Prof. Dra. Carla Freire Celedônio Fernandes. No Brasil cerca de 7,5 % dos casos de envenenamento ofídico são causados por serpentes do gênero Crotalus, os quais originam uma taxa de mortalidade de 1,8 %. Os efeitos neurotóxicos, miotóxicos e nefr...

  4. Irradiação global, direta e difusa, para a região de Cascavel, Estado do Paraná = Global, direct and diffuse irradiation in the region of Cascavel, Paraná State

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    Patricia Aparecida de Oliveira Drechmer

    2006-01-01

    Full Text Available O presente trabalho apresenta um estudo sobre os níveis das irradiações global, direta e difusa incidentes na superfície terrestre, a irradiação incidente no topo da atmosfera e a insolação relativa mensal, para a cidade de Cascavel-PR. As componentes da radiação solar foram monitoradas entre janeiro de 2001 e fevereiro de 2003 na Estação Experimental Agrometeorológica da Unioeste, Cascavel, Estado do Paraná. Na medição da radiação global foi utilizado um piranômetro KIPP & ZONEN-CM3 e para medir a componente direta foiutilizado um pireliômetro EPPLEY-NIP acoplado a um rastreador solar. A radiação difusa foi calculada pela diferença entre as radiações global e direta. Os níveis médios mensais das irradiações global, direta e difusa, foram respectivamente, 51,8%, 31,7% e 20,17% dairradiação incidente no topo da atmosfera. O índice de insolação relativa apresentou valor máximo em agosto (61,43% e mínimo em fevereiro (34,35%, períodos caracterizados respectivamente por dias de céu limpo e nublados no hemisfério sul.This paper reports a study about the diffuse, direct and global radiation levels on surface of earth, the irradiation over the top of atmosphere and the monthly relative sunstroke in the city of Cascavel, State of Paraná, southern Brazil. The solar radiation components were monitored from January, 2001 to February, 2003 at Estação Experimental Agrometeorológica of Unioeste, Cascavel, Parana State. Global radiation was monitored by a pyranometer KIPP & ZONEN-CM3 and direct radiation was monitored by a pyrheliometer EPPLEY-NIP coupled to a solar tracer. The diffuse radiation wasmonitored through the difference between global and direct radiation. Monthly average levels of global, direct and diffuse irradiations were 51.8%, 31.7% and 20.17% of the irradiation in the top of atmosphere. The relative isolation index showed the maximum value in August (61.43% and the minimum in February (34.35%. These

  5. Is the population of Crotalus durissus (Serpentes, Viperidae) expanding in Brazil?

    Science.gov (United States)

    Duarte, Marcelo Ribeiro; Menezes, Frederico Alcântara

    2013-12-05

    Crotalus durissus are found from Mexico to northern Argentina in a highly disjunct distribution. According to some studies, this species is prone to occupy areas disturbed by human activities and floods comprise a plausible method of dispersal as inferred for some North American rattlesnakes. Based on the literature, it seems plausible that Crotalus durissus expanded their natural distribution in Brazil due to floods, but only in a few municipalities in Rio de Janeiro State. Data entries of Butantan Institute, in São Paulo, Brazil, from 1998 to 2012 show a declining tendency of snakes brought by donors. In addition, research shows no evidence of Crotalus durissus being an expanding species in the Brazilian territory.

  6. Atendimento de crianças realizado pelo SIATE de Cascavel no ano de 2004

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    Márcio Briccius

    2008-03-01

    Full Text Available Os acidentes com crianças vêm-se constituindo, cada vez mais, em uma relevante causa de morbimortalidade. As injúrias físicas respondem por 98% das causas de mortes nos países em desenvolvimento. O estudo teve como objetivo, identificar os tipos de acidentes envolvendo crianças de zero a doze anos atendidas pelo SIATE de Cascavel. Trata-se de estudo descritivo, desenvolvido com a consulta realizada aos arquivos do SIATE, sobre os atendimentos realizado à crianças no ano de 2004. Obteve-se como resultado o registro de 379 atendimentos envolvendo crianças. As principais causas foram os acidentes de trânsito (63,6% e a queda (20,3%, atingindo meninos (63,6% com idade entre 10 a 12 anos (33%. Dentre os acidentes de trânsito a colisão (31,1% e o atropelamento (18,2% foram os mais freqüentes. Já no ambiente domiciliar os atendimentos foram devidos a destelhamentos, queda de tanque de lavar roupas, queimaduras, afogamentos, intoxicações e eventos envolvendo cavalo e abelha. Houve ainda registro devido a agressão, estupro e ferimento por arma de fogo. Conclui-se que, as crianças têm sofrido injúrias diversas decorrentes dos acidentes de trânsito, doméstico além de violências físicas e sexuais, indicando a necessidade de intensificar as estratégias voltadas à prevenção de acidentes de trânsito.

  7. Movimentos pendulares das cidades interioranas de porte médio de Cascavel e Toledo, no Paraná Movimientos pendulares de las ciudades del interior de porte medio de Cascavel y Toledo, en Paraná Commuting in medium-size cities of Cascavel and Toledo, Paraná

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    Cristiano Stamm

    2008-06-01

    Full Text Available O objetivo desse artigo é examinar o fenômeno do movimento pendular de trabalhadores intermunicipais entre as cidades interioranas de porte médio de Cascavel e Toledo, localizadas na Mesorregião Oeste do Estado do Paraná. A dinâmica econômico-populacional das distintas regiões do interior do Brasil vem imprimindo novas modalidades de deslocamentos populacionais, as quais eram quase exclusivas dos grandes centros urbanos. Os estudos dessa natureza tornam-se importantes, pois levantam informações relevantes para o planejamento urbano-regional e no ordenamento da dinâmica territorial. O movimento pendular observado nesse estudo é do tipo cruzado, o que significa que há movimento casa-trabalho originado nas duas cidades, em razão de ambas serem pólos regionais. O resultado da pesquisa evidenciou que a rede primária de proteção social (a família é um fator importante e, em particular, das cidades de porte médio, como é caso de Cascavel e Toledo. O fluxo de trabalhadores pendulares entre Cascavel e Toledo apresenta um papel fundamental no desenho da rede urbana da região. Desta forma, verificou-se que algumas questões urbanas apresentadas nos grandes centros urbanos e nas regiões metropolitanas do país, tais como os movimentos pendulares, são também temas importantes para as cidades de menor porte populacional.El objetivo de este artículo es examinar el fenómeno del movimiento pendular de trabajadores intermunicipales entre las ciudades del interior de porte medio de Cascavel y Toledo, localizadas en la Mesoregión Oeste del Estado de Paraná. La dinámica económico-poblacional de las distintas regiones del interior de Brasil viene imprimiendo nuevas modalidades de desplazamientos poblacionales, las cuales eran casi exclusivas de los grandes centros urbanos. Los estudios de esta naturaleza se tornan importantes, pues exponen informaciones relevantes para la planificación urbano-regional y en el ordenamiento de la din

  8. Evidência sorológica da infecção aguda pelo Toxoplasma gondii em gestantes de Cascavel, Paraná Serological evidence of acute Toxoplasma gondii infection in pregnant women in Cascavel, Paraná

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    Sônia de Lucena Mioranza

    2008-12-01

    Full Text Available Para verificar a ocorrência da toxoplasmose em Cascavel, Paraná, cidade próxima a região onde ocorreu o maior surto epidêmico descrito mundialmente, 334 amostras de soros de gestantes foram triadas pelo ensaio imunoenzimático comercial IgG no Laboratório Municipal de Cascavel, e confirmadas no Instituto de Medicina Tropical de São Paulo por imunofluorescência IgG, ensaio imunoenzimático e avidez de IgG in house. A soropositividade pelo IgG comercial foi 54,2%, pela imunofluorescência 54,8% e pelo IgG in house 53,9%, com boa concordância entre imunofluorescência/IgG comercial (Kappa=0,963781; co-positividade=97,8%; co-negatividade=98,7% e imunofluorescência/IgG in house (Kappa=0,975857; co-positividade=97,8%; co-negatividade=100%. A evidência de infecção aguda nas gestantes foi similar tanto pela avidez de IgG (2,4% ao ano como pela análise estatística de tendência (teste χ2 por faixa etária (2% ao ano, sugerindo que a triagem sorológica pré-natal e a vigilância epidemiológica são imprescindíveis para redução do risco da toxoplasmose na região, embora sem evidência de surto epidêmico.In order to investigate the incidence of toxoplasmosis in Cascavel, Paraná, a city near the region where the largest reported epidemic outbreak in the world occurred, 334 serum samples from pregnant women were screened using a commercial IgG immunoenzymatic assay at the Municipal Laboratory in Cascavel and were confirmed at the Institute of Tropical Medicine in São Paulo, by means of IgG immunofluorescence, immunoenzymatic assaying and the in-house IgG avidity test. The IgG seropositivity from the commercial test was 54.2%, from immunofluorescence 54.8% and from the in-house IgG 53.9%, with good agreement between immunofluorescence and the commercial IgG test (kappa = 0.963781; co-positivity = 97.8%; co-negativity = 98,7% and between immunofluorescence and the in-house IgG (kappa = 0.975857; co-positivity = 97.8%; co

  9. Avaliação qualitativa de pulverizadores da região de Cascavel, Estado do Paraná = Sprayers qualitative evaluation in chemical application in Cascavel, State of Paraná

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    João Cleber Modernel da Silveira

    2006-10-01

    Full Text Available No processo de tratamento fitossanitário, o controle das características da pulverização deve ser realizado com o objetivo de garantir a eficiência dos tratamentos, evitando-se perdas de produtos sem causar danos ao meio e ao próprio homem. Dessa forma, é fundamental que os pulverizadores estejam em perfeito estado de conservação. O objetivo deste trabalho foi realizar na região de Cascavel, Estado do Paraná, uma avaliação qualitativa de pulverizadores agrícolas. Foram checados, no campo, o estado e presença dos principais componentes de 62 pulverizadores. Os pulverizadores avaliados foram divididos em três categorias: montado, arrasto e autopropelido. Observou-se que os equipamentos avaliadosestão com manutenção inadequada. Considerando a presença e o estado de alguns componentes (bicos, manômetros, filtros de linhas, entre outros, apenas 17% dos equipamentos estão em condições adequadas de uso.Spray quality control should be accomplished in the process of chemical treatment, aiming to guarantee the efficiency, avoiding loss of product and damages to the environment. It is fundamental, thus, sprayers optimum conservation. This work aimed to carry out a qualitative evaluation of the sprayers in farms in the municipality of Cascavel, State of Paraná, southern Brazil. The conditions and presence (or absence of main components in 62 sprayers were checked in the field. The appraised sprayers were divided in three categories: attached to tractors, PTO-driven trailed and self-propelled. The equipments showed inadequate maintenance. Considering the presence and the conditions of some components (nozzles, filters of lines, nozzles filters, among others, only 17% of the equipment evaluated was in good condition.

  10. Avaliação socioeconômica e de fatores que levam a violência com detentos de Cascavel/PR Evaluación socio-económica y de factores que levan a la violencia com detenidos de Cascavel/PR Socioeconomic and factor evaluation that lead to violence with inmates of Cascavel/PR

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    Thiago Eugênio Armani

    2010-06-01

    Full Text Available A violência não é caracterizada apenas por sequestros, roubos, assaltos, mas também por se destruir a identidade social, cultural e econômica de um povo. A violência aprisiona a capacidade de um país evoluir, porque coloca seus jovens no mundo das drogas, da prostituição, do analfabetismo, enfim na marginalização social, pois todo o desenvolvimento de um povo só acontece graças a uma evolução social, cultural, econômica, psíquica e biológica, que é ditada principalmente pela educação empregada nas escolas. Baseado nisto este trabalho analisou as características sociais, educacionais e econômicas de sujeitos entre 18 e 60 anos que se encontram em cárcere privado na cidade de Cascavel/PR. Através da aplicação de um questionário com perguntas e respostas objetivas, que a partir do seu preenchimento forneceu ideia de quais são os possíveis fatores geradores de violência, com a pesquisa sendo realizada diretamente com os detentos. Foi constatado que há problema educacional, social e econômico com os detentos cascavelenses, porque os mesmos possuem pouca escolaridade, sofrem com desemprego, baixa renda, entre outros problemas, que influenciam a entrada para a criminalidade.La violencia no sólo se caracteriza por los secuestros, robos, asaltos, sino también por la destrucción de la identidad social, cultural y económica de las personas. La violencia aprisiona la capacidad de un país evolucionar por que coloca sus jóvenes en el mundo de las drogas, de la prostitución, del analfabetismo, en fin en la marginalización social, pues todo el desarrollo de un pueblo sólo pasa a través de la evolución social, cultural, económica, psicológica y biológica, que es dictada principalmente por la educación en las escuelas. Este trabajo analizó las características sociales, educacionales y económicas de sujetos entre 18 y 60 años que están en una casa de detención en la ciudad de Cascavel/PR. Mediante la aplicaci

  11. Formas de uso medicinal da babosa e camomila pela população urbana de Cascavel, Estado do Paraná = Medicinal use of the Aloe and Chamomile for the urban population of Cascavel, Paraná State

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    Claudia Tatiana Araujo da Cruz-Silva

    2010-07-01

    Full Text Available A etnobotânica se caracteriza por buscar entender a relação entre as plantas e o homem, podendo-se através desta conhecer a utilização das plantas medicinais como forma de tratamento. O objetivo deste trabalho foi realizar um levantamento sobre as formas de utilização da Babosa (Aloe vera L. e da Camomila (Matricaria chamomilla L.. A coleta de dados envolveu entrevistas de forma aleatória a 400 pessoas, através de um questionário semi-estruturado, no município de Cascavel, Estado do Paraná. Observou-se que 65% da população utilizam à babosa ou a camomila na cura ou alívio de doenças. A maioria dosentrevistados possui renda de 2 a 4 salários mínimos (60,25%, idade entre 28 a 38 anos (30,75%, tendo concluído o ensino médio (33,75%. O principal motivo pelo qual a população se utiliza de plantas medicinais é por ser natural (71,84%. A forma de preparo mais freqüente da camomila foi por infusão (63,38%, utilizando as flores (92%. Para a babosa 100% utilizam suas folhas na forma de cataplasma (43,88%. Apenas 3% da população relataram ter apresentado algum tipo de reação adversa, durante o período de utilização. Conclui-se que o uso destas plantas pela população é freqüente, sendo um recurso adicional ao uso de medicamentos.The ethnobotany, which is characterized by look for to understand the relationship between the plants and the man, being been able through this to know the use of the medicinal plants as treatment. The objective of this work was to accomplish a rising on the forms of use of the Aloe (Aloe vera L. and of the Chamomile (Matricaria chamomilla L.. The collection of data involved interviews in a random way to a sample of 400 people through a semi-structured questionnaire, in the city of Cascavel, Paraná State. It was observed that 65% of the population use to aloe or the chamomile in the cure or relief of diseases. Most of the interviewees possesses income from 2 to 4 minimum wages (60.25%, age

  12. Eficiência de um conjunto fotovoltaico em condições reais de trabalho na região de Cascavel = Efficiency of the photovoltaic systems under real work conditions on region of Cascavel

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    Estor Gnoatto

    2008-07-01

    Full Text Available No presente trabalho, foi desenvolvido um estudo sobre um sistemafotovoltaico autônomo, com armazenamento de energia, instalado na Estação Experimental Agrometeorológica da Universidade Estadual do Oeste do Paraná, Campus Cascavel, a uma Latitude 24º59’ Sul, Longitude de 53º26’ Oeste, com altitude de 682 m, em condições reaisde campo, durante o ano de 2002. Foi avaliada a eficiência dos painéis fotovoltaicos em condições reais de campo, sua curva característica foi determinada utilizando, na aquisição de dados, um micrologger da Campbell Scientific-Inc, modelo CR10X, programado pararealizar uma leitura por segundo de cada canal e armazenar a média aritmética de 5 minutos dos dados das componentes de radiação solar global no plano horizontal e global na incidência do painel; temperatura ambiente e temperatura de operação do painel; tensão ecorrente do sistema fotovoltaico. Os resultados mostram que a eficiência média mensal registrou valores na faixa de 8,65 a 9,17%, com uma média de 8,84%, com variação percentual entre o índice máximo e mínimo de 5,67%. Pode, assim, representar fornecimento médio mensal de energia de 5,57 kWh por painel.The purpose of the present work was to develop a study of anautonomous photovoltaic system, with energy storage, installed at the Experimental Agrometeorological Station of the Universidade Estadual do Oeste do Paraná, Cascavel Campus, located at 24º59’S 53º26’W, 682 meters of altitude, under real field conditions, during the year of 2002. The efficiency of the photovoltaic modules was evaluated underreal field conditions and its characteristic linear fit was determined. A Campbell Scientific CR10X micrologger was used to acquire data, programmed to conduct one reading per second from each channel and store the five-minute arithmetic data average from thereadings of the incidence of global direct solar radiation horizontal and global on the panel, as well as area

  13. Crescimento, composição corporal, desempenho motor de escolares de 07a 10 de idade do municipio de Cascavel-Parana

    OpenAIRE

    Evandro Rogerio Roman

    2004-01-01

    Resumo: O objetivo deste estudo foi analisar o crescimento físico, composição corporal e desempenho motor de escolares de 7 a 10 anos, de ambos os gêneros. Para tanto, a amostra foi constituída por 1.267 escolares, sendo 654 meninos e 613 meninas, pertencentes à pública de ensino do município de Cascavel-PR. Medidas antropométricas de massa corporal, estatura e espessura de dobras cutâneas foram obtidas para a determinação do índice de massa corporal (IMC), da gordura corporal relativa e da m...

  14. A "Pedagogia" da Feira Livre de SÃo Bento: narrativas, saberes e prÃticas educativas na cidade de Cascavel - CE

    OpenAIRE

    Francisca Eliana Santos da Silva

    2014-01-01

    Neste estudo, buscamos compreender as prÃticas educativas e os saberes presentes na feira livre de SÃo Bento, localizada na cidade de Cascavel-CE a qual dista de Fortaleza 64 km. Para a realizaÃÃo dessa pesquisa, escolhemos como suporte teÃrico BrandÃo (2005); Durkheim (2007); Burke (2003); Huberman (1976) e Certeau (1998). Optamos pelo enfoque qualitativo e utilizamos a pesquisa bibliogrÃfica como suporte no esclarecimento do tema investigado. Usamos tambÃm o estudo de caso que proporcionou ...

  15. Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Alessandra Snak

    2015-12-01

    Full Text Available Digestive and respiratory tracts parasite’s protozoan, Cryptosporidium spp. now-a-days is a major zoonotic agent, it causes self-limiting diarrhea, remaining in the body passively until the moment immune system decreases, leading to an increase in its multiplication in the mucosa and appearance of clinical signs. As there are few studies on cryptosporidiosis in wild free-living and captive animals, especially in Brazil, this study aimed to identify the presence of Cryptosporidium spp. in feces of captive animals in Cascavel, PR Municipal Zoo. Between 2011 and 2012 there have been four collections of bird feces and five mammalian feces totaling 65 and 118 samples respectively. Samples were sent to the laboratory of Veterinary Parasitology of Federal University of Parana. The feces were diluted, centrifuged and the pellets were used to make blades which were stained by the Ziehl-Neelsen modified method and observed under a microscope with 1000X magnification. Then the blades containing the oocysts were observed under a capture microscope, where they were measured. Mammals showed 49.15% and birds 44.61% of positivity. Oocysts’ sizes ranged from 3,54?m to 5,81?m with an average of 4,32?m for birds and 3,11?m to 5,60?m averaging 4,63?m to mammals. As of yet, there isn’t effective treatment against this parasite and considering that it’s a zoonotic disease, preventive measures should be taken to prevent transmission to humans.

  16. The adrenergic retulation of the cardiovascular system in the South American rattlesnake, Crotalus durissus

    DEFF Research Database (Denmark)

    Galli, G.L.J.; Jensen, Nini Skovgaard; Abe, A.S.

    2007-01-01

    The present study investigates adrenergic regulation of the systemic and pulmonary circulations of the anaesthetised South American rattlesnake, Crotalus durissus. Haemodynamic measurements were made following bolus injections of adrenaline and adrenergic antagonists administered through a systemic...... arterial catheter. Adrenaline caused a marked systemic vasoconstriction that was abolished by phentolamine, indicating this response was mediated through α-adrenergic receptors. Injection of phentolamine gave rise to a pronounced vasodilatation (systemic conductance (Gsys) more than doubled), while...... injection of propranolol caused a systemic vasoconstriction, pointing to a potent α-adrenergic, and a weaker β-adrenergic tone in the systemic vasculature of Crotalus. Overall, the pulmonary vasculature was far less responsive to adrenergic stimulation than the systemic circulation. Adrenaline caused...

  17. Daily and seasonal activity patterns of free range South-American rattlesnake (Crotalus durissus

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    ALEXANDRO M. TOZETTI

    2013-09-01

    Full Text Available This study aimed at describing daily and seasonal variation in the activity of a population of South-American rattlesnakes (Crotalus durissus in a savanna like habitat (Cerrado in Southeastern Brazil. Seasonal and daily activities of snakes were evaluated by the number of captures of snakes during road surveys, accidental encounters, and relocations by radio-tracking. Our results show that climatic variables such as air temperature and rainfall have little influence on the activity pattern of rattlesnakes. Our findings indicate that rattlesnakes spend most of the day resting and most of the night in ambush posture. The South-American rattlesnake is active throughout the year with a discrete peak in activity of males during the matting season. The possibility of maintaining activity levels even during the coldest and driest season can facilitate the colonization of several habitats in South America. This possibility currently facilitates the colonization of deforested areas by rattlesnakes.

  18. Características dos doadores de córneas do Banco de Olhos de Cascavel: impacto do exame anti-HBc para hepatite B

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    Cesar Nobuo Shiratori

    2011-02-01

    Full Text Available OBJETIVOS: Traçar o perfil dos doadores de tecidos oculares humanos na região de atuação do Banco de Olhos de Cascavel - Paraná (PR e verificar o impacto da positividade da sorologia positiva para hepatite B (anti-HBc no descartes dos tecidos oculares. MÉTODOS: Estudo do tipo transversal de dados do Banco de Olhos de Cascavel do período de março de 2006 a abril de 2007. Informações como idade, sexo, causa de óbito, tempo entre óbito e enucleação e resultados sorológicos de doadores foram coletadas. Dados foram agrupados para caracterização da população de estudo e distribuídos segundo positividade da sorologia para hepatite B (anti-HBc. RESULTADOS: A média de idade dos doadores foi de 54,2 ± 20,6 anos e a maioria era do sexo masculino (64,7%. Tempo médio entre óbito e enucleação foi de 3,8 ± 2,4 horas, a principal causa de óbito foram doenças cardiovasculares (34,5%. Positividade para os marcadores anti-HBc e HBsAg foi de 47,4% e 1,5%, respectivamente, sendo os exames sorológicos para hepatite B a maior causa de descarte dos tecidos oculares doados. Doadores com sorologia positiva para anti-HBc apresentaram maior média de idade comparados com doadores com sorologia negativa (p<0,001. Somente 0,8% das doações apresentaram sorologia positiva para hepatite C. Não houve doações com sorologia para HIV positiva no período do estudo. CONCLUSÕES: Implementação do Banco de Olhos de Cascavel aumentou significativamente a disponibilização de córneas para transplante no Estado do Paraná. A principal contraindicação para disponibilização de tecidos oculares foi a positividade na sorologia para anti-HBc. Estudos de biosegurança devem ser realizados a fim de investigar o potencial de transmissão da hepatite B de doadores positivos para esse marcador. A inclusão desses doadores como doações efetivas acarretaria maior disponibilização de tecidos para transplantes.

  19. Avaliação dos níveis de iluminação natural e artificial nas residências convencional e inovadora do ‘Projeto CASA’, Unioeste, campus de Cascavel, Estado do Paraná = Evaluation of natural and artificial lighting in conventional and innovative residences of ‘Projeto CASA’, Unioeste, Cascavel Campus, Paraná

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Camargo Nogueira

    2010-07-01

    Full Text Available Este trabalho teve por objetivo comparar os níveis de iluminação natural e artificial nas residências convencional e inovadora do ‘Projeto CASA’ (Centro de Análise de Sistemas Alternativos de Energia da Unioeste – campus de Cascavel. As medidas foram realizadas com a utilização de um Luxímetro digital, nos diversos cômodos das residências, e em diferentes horas do dia. Como resultado, verificou-se que a residência inovadora apresentou maior uniformidade na distribuição da iluminância, estando seus níveisadequados aos valores mínimos estabelecidos na NBR 5413.The purpose of this work was to compare the natural and artificial lighting levels in the conventional and innovative residences of ‘Projeto CASA’ (Center for the Analysis of Alternative Energy Systems of Unioeste – Cascavel campus. The measures were carriedout using a digital light meter, in the several rooms of the residences, at different hours of the day. It was verified that the innovative residence presented a larger uniformity in lighting distribution, being in agreement with the minimum values established in NBR 5413.

  20. Estudo clínico e laboratorial de pacientes com artrite reumatoide diagnosticados em serviços de reumatologia em Cascavel, PR, Brasil Clinical and laboratory features of patients with rheumatoid arthritis diagnosed at rheumatology services in the Brazilian municipality of Cascavel, PR, Brazil

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    Juliano Maximiano David

    2013-02-01

    Full Text Available INTRODUÇÃO: Estudos epidemiológicos brasileiros sobre artrite reumatoide são bastante escassos, e os dados existentes hoje são majoritariamente de literatura internacional. OBJETIVOS: Determinar a incidência e algumas características clínicas e laboratoriais de pacientes com artrite reumatoide em Cascavel, PR, Brasil. PACIENTES E MÉTODOS: Os dados foram coletados entre agosto de 2010 e julho de 2011 em todos os serviços de saúde do município que possuíam atendimento na especialidade de reumatologia: um hospital universitário, o Centro Regional de Especialidades do Consórcio Intermunicipal de Saúde do Oeste do Paraná (CRE-CISOP e quatro clínicas privadas da cidade. RESULTADOS: Foram identificados 38 pacientes com diagnóstico de artrite reumatoide, resultando em uma incidência estimada de 13,4 casos/100.000 habitantes/ano. Trinta e dois pacientes eram do gênero feminino, com média de idade de 47,6 anos. A faixa etária com maior incidência foi > 40 anos. O tempo médio entre os primeiros sintomas e o diagnóstico foi de 12,4 meses. O fator reumatoide foi positivo em 68,4% dos casos, e 18,4% já apresentavam alterações radiológicas no momento do diagnóstico. O tratamento farmacológico dos pacientes também foi avaliado e mostrou estar de acordo com o encontrado na literatura. CONCLUSÃO: A incidência de artrite reumatoide obtida em Cascavel está abaixo das incidências observadas em estudos internacionais.INTRODUCTION: Brazilian epidemiological studies on rheumatoid arthritis are scarce, thus all data currently available originate from the international literature. OBJECTIVES: To determine the incidence and some clinical and laboratory characteristics of patients with rheumatoid arthritis in the municipality of Cascavel, state of Paraná, Brazil. PATIENTS AND METHODS: Data were collected between August 2010 and July 2011 in all health services of the municipality of Cascavel that provided health care in Rheumatology: a

  1. Strategy for subduing prey in the predatory behavior of Crotalus durissus collilineatus Amaral, 1926, in captivity

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    Vera Lucia de Campos Brites

    2006-12-01

    Full Text Available Studies of the predatory behavior of six specimens of hatchlings from the same clutch of Crotalus durissus collilineatus, born and raised in captivity, were conducted. The animal focal technique (observing the snakes through a glass visor was used in studying the snakes in order to minimize stress. The snakes were fed mice sequentially and were observed until they no longer sought the prey. Different capture strategies were adopted by this subspecies after the first occasion on which prey was caught. Percentages of the total number of capture strategies observed indicate that 20% (n = 4 made use of seizure, and 80% (n = 16 made use of poisoning, as a mean of subduing prey. The strategy of seizing prey manifested itself on the third or fourth occasion that prey was offered and appeared to be linked to the small size of the prey. It was observed that in 95% (n = 20 of the cases, ingestion began with the cranium of the prey. Regardless of the body part that was ingested first by the serpent, all serpents displayed adjustment behavior of the cranium/head and the jaws (“yawning”.

  2. Gross and histologic features of gastritis due to Ophidascaris arndti in tropical rattlesnakes ( Crotalus durissus

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    É.M. Mello

    Full Text Available ABSTRACT The tropical rattlesnake (Crotalus durissus is a snake of great importance for biomedical industry since its poison is used for the production of antiophidic serum and researches. Several conditions related to animal health, such as diseases and parasites, which can promote the reduction of poison production by these snakes should be investigated. Accordingly, the aim of this study was to characterize the gross and microscopic lesions related to the presence of Ophidascaris arndti in stomachs of tropical rattlesnakes. The gastrointestinal tract of thirty-five South American rattlesnakes captured in Southeastern region of Brazil were analyzed and nineteen animals showed infestation by the parasites, found in the small intestine and, especially, in the stomach of the hosts. Grossly, lesions were characterized by mucosal ulcers occasionally associated with hemorrhage. Microscopic alterations included histiocytic granulomas, fibrosis, necrosis, and hemorrhage. Based on these findings, the diagnosis of a parasitic granulomatous disease was made. The lesions may be related to the cause of death in captivity snakes, since the lesions can promote secondary infections by opportunistic bacteria. Moreover, the intense inflammatory response accompanied by fibrosis may be related to poor functioning of the gastric snakes, which it may exhibit frequent regurgitation of the food.

  3. Comprometimento respiratório secundário a acidente ofídico crotálico (Crotalus durissus Respiratory abnormalities following Crotalus durissus snakebite

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    Carlos Faria Santos Amaral

    1991-08-01

    Full Text Available São analisados três pacientes que apresentaram comprometimento da função respiratória após acidente por Crotalus durissus. As manifestações respiratórias surgiram nas primeiras 48 horas após a picada do ofídio e consistiram de dispnéia, taquipnéia, uso da musculatura acessória da respiração (casos 1 e 2 e batimento das aletas nasais (caso 2. Dois pacientes (casos 1 e 2 apresentaram insuficiência respiratória aguda. O diagnóstico desta complicação no caso 1 foi clínico pois o paciente apresentou apnéia. O paciente do caso 2, 24 horas após o acidente ofídico apresentou dificuldade respiratória intensa e períodos de apnéia sendo intubado, permanecendo em respiração espontânea. Houve agravamento dos sinais clínicos de insuficiência respiratória e a determinação de pH e gases do sangue arterial mostrou em relação ao exame inicial elevação da pressão parcial de gás carbônico (40 mmHg para 50,3 mm Hg caracterizando insuficiência ventilatória aguda. Ambos foram tratados com emprego de ventilação artificial mecânica, tendo o paciente do caso 1 permanecido no ventilador durante 33 dias e o do caso 2 durante 15 dias. Ambos desenvolveram insuficiência renal aguda, necessitaram de diálise peritoneal e recuperaram a função renal. A paciente do caso 3, apesar dos sintomas e sinais de comprometimento respiratório não apresentou alterações do pH e gases arteriais. Espirometria realizada 58 horas após o acidente mostrou capacidade vital forçada (CVF e volume espirado no primeiro segundo (VEF1 abaixo do previsto (60 e 67% respectivamente. As espirometrias realizadas nos dias subseqüentes evidenciaram melhora progressiva destes parâmetros. No 10º dia após o acidente constatou-se aumento de 20% da CVF e de 17% do VEF1 comparativamente ao exame inicial. A relação entre VEF1 e a CVF manteve-se praticamente inalterada e em valores próximos ao previsto, caracterizando distúrbio ventilatório do tipo

  4. Estimativa da irradiação solar global com partição mensal e sazonal para a região de Cascavel - PR Evaluation of global solar irradiation with sazonal and monthly partition for the area of Cascavel - PR, Brazil

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    Melania I. Valiati

    2005-04-01

    Full Text Available No presente trabalho, mostram-se equações de estimativa da irradiação solar global (R G, por meio do modelo de Angstrom, com partições sazonal e mensal para a região de Cascavel - PR. Os dados experimentais foram cedidos pelo IAPAR, coletados na sua estação meteorológica localizada na COODETEC/Cascavel - PR, no período de 1983 a 1998. Dos 16 anos de dados, 12 anos foram utilizados para cálculo dos coeficientes (a e b e quatro anos para a validação das equações. Os coeficientes de determinação encontrados foram superiores a 80% para as duas partições. O mínimo da R G é superestimado e o máximo é subestimado quando comparados com o mínimo e o máximo para dados reais, sendo esses encontrados no solstício de inverno e equinócio de primavera, respectivamente. A variação sazonal e mensal do coeficiente "a" foi menor (0,16 a 0,19 e 0,14 a 0,21 e do coeficiente "b" maior (0,34 a 0,43 e 0,32 a 0,44. As maiores variações dos erros médios diários ocorreram no equinócio de primavera (-19,45% a 27,28% e as menores no equinócio de outono (-11,32% a 10,61%. O ajuste mais eficaz das equações foi encontrado para a partição mensal.This research shows equations of evaluation of global solar irradiation (GR through Angstrom's model with seasonal monthly partitions for the region of Cascavel -PR, Brazil. The experimental data were given by IAPAR and collected in its meteorological station located in COODETEC/Cascavel - PR, Brazil, from 1983 to 1998. Twelve of the sixteen years of data were used for calculation of the coefficients (a and b and four years for the validation of the equations. The determination coefficients found were superior to 80% for both partitions. The minimum (GR is overestimated and the maximum is underestimated when compared to the minimum and maximum for real data, been these found in the winter solstice and spring equinox, respectively. The seasonal and monthly variation of the coefficient "a" was lower

  5. Avaliação farmacognóstica e microbiológica da droga vegetal camomila (Chamomilla recutita L. comercializada como alimento em Cascavel - Paraná Pharmacognostic and microbiological evaluation of the medicinal plant chamomile (Chamomilla recutita L. commercialized as food in Cascavel Municipality, Paraná State, Brazil

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    P.S.R Lucca

    2010-06-01

    Full Text Available As plantas medicinais, dentre elas a camomila, têm sido muito utilizadas na forma de droga vegetal pela população em geral, como importantes alternativas alimentícias e terapêuticas. Considerando que a fiscalização sanitária destes produtos é precária, este quadro torna-se preocupante, visto que, um produto em condições inadequadas para consumo pode acarretar vários riscos ao consumidor. Diante disso, este trabalho teve por objetivo realizar avaliação farmacognóstica e microbiológica em quinze amostras de chá de camomila comercializadas na cidade de Cascavel, Paraná. A metodologia adotada para a realização dos testes foi a preconizada pela Farmacopéia Brasileira (1988, 1996, 1998. O estudo de autenticidade revelou que todas as amostras eram constituídas por Chamomilla recutita L., porém na maioria das amostras os capítulos florais apresentavam-se excessivamente destruídos. Seis amostras apresentaram teor de materiais estranhos acima de 5%. Quanto ao doseamento de óleo essencial, todas as amostras foram insatisfatórias, visto que, apresentaram apenas traços de óleos essenciais, ou seja, valores abaixo de 0,4%, sendo este valor o mínimo exigido pela Farmacopéia. Com relação às análises de bolores e leveduras foi verificado que quatro amostras apresentaram valores acima do limite de 10(4 UFC mL-1, conforme preconizado pela Organização Mundial de Saúde. A partir dos dados obtidos concluiu-se que a camomila comercializada na cidade de Cascavel - Paraná apresenta problemas com relação à qualidade, sendo necessário definir medidas adequadas de controle higiênico sanitário ao longo da cadeia de produção, para garantir a qualidade e segurança destes produtos.Medicinal plants, including chamomile, have been largely used by the general population as important food and therapeutic alternatives. Considering that the sanitary control of such products is precarious, this situation becomes worrisome since a

  6. AVALIAÇÃO NUTRICIONAL DE PRATICANTES DE MUSCULAÇÃO COM OBJETIVO DE HIPERTROFIA MUSCULAR DO MUNICÍPIO DE CASCAVEL, PR

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    Ana Flávia de Oliveira1

    2009-06-01

    Full Text Available This study aimed to assess diet and investigate the use of nutritional supplements and anabolic steroids in practicing workout. Eleven male gender individuals were evaluated for who practice workout with the objective of muscle hypertrophy of the municipality of Cascavel – Paraná. The dietary evaluation was carried out through dietary investigators. The micronutrients intake, macronutrients and dietary fibers were analyzed according to the Recommended Dietary Allowances. It was collected information about the use of nutritional supplements and anabolic steroids. In regard to the nutritional orientation only four (36.4% individuals have received this type of service. Regarding the use of food supplements, five (45.5% make use, and cited seven different types. Regarding the use of anabolic steroids three of the individuals (27.3% reported using or have already made use Eight (72.7% had energy consumption more than 3000 kcal/d and the average of micronutrients intake presented a non balanced diet, low in carbohydrates (40.2 ± 8.4% and high in fat (36.0 ± 5.9%. Through this study, it was observed that the individuals evaluated are conscious about the appropriate food to obtain muscle hypertrophy, there is erroneous eating habits, indicating possible risks to health in the long term and even decrease in athletic performance.

  7. Os jornais diários da cidade de Cascavel (PR e seus discursos sobre a prostituição feminina (1976-1990

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    Fábio Lopes Alves

    2010-01-01

    Full Text Available The object of the present paper is to investigate the representations of female prostitution in the periodical press of Cascavel (PR, Brazil. The journals analyzed are the newspapers Hoje and O Paraná. It aims to understand how some women, who do not figure in the annals of the feminist movement, that don't have an exceptional and exemplary trajectory to the young ladies and not even show up on the social columns of the press, are represented by these media. In this context, this discussion is structured as follows, at first, it establishes a dialog with Roger Chartier to reach the representation concept. After that, the subject will be about how the female prostitution was represented by written press. At this point, the article highlights the journalistic reports that alluded to the risk of social contagion offered by these women to the society, while they are catching the AIDS and syphilis virus. At the end, it interacts with the philosopher Michel Foucault, to realize how the disciplining of the bodies through journalistic speeches happened.

  8. Tracing an invasion: landbridges, refugia, and the phylogeography of the Neotropical rattlesnake (Serpentes: Viperidae: Crotalus durissus).

    Science.gov (United States)

    Wüster, Wolfgang; Ferguson, Julia E; Quijada-Mascareñas, J Adrian; Pook, Catharine E; Salomão, Maria da Graça; Thorpe, Roger S

    2005-04-01

    Abstract Pleistocene fragmentation of the Amazonian rainforest has been hypothesized to be a major cause of Neotropical speciation and diversity. However, the role and even the reality of Pleistocene forest refugia have attracted much scepticism. In Amazonia, previous phylogeographical studies have focused mostly on organisms found in the forests themselves, and generally found speciation events to have predated the Pleistocene. However, molecular studies of open-formation taxa found both north and south of the Amazonian forests, probably because of vicariance resulting from expansion of the rainforests, may provide novel insights into the age of continuous forest cover across the Amazon basin. Here, we analyse three mitochondrial genes to infer the phylogeography of one such trans-Amazonian vicariant, the Neotropical rattlesnake (Crotalus durissus), which occupies primarily seasonal formations from Mexico to Argentina, but avoids the rainforests of Central and tropical South America. The phylogeographical pattern is consistent with gradual dispersal along the Central American Isthmus, followed by more rapid dispersal into and across South America after the uplift of the Isthmus of Panama. Low sequence divergence between populations from north and south of the Amazon rainforest is consistent with mid-Pleistocene divergence, approximately 1.1 million years ago (Ma). This suggests that the Amazonian rainforests must have become fragmented or at least shrunk considerably during that period, lending support to the Pleistocene refugia theory as an important cause of distribution patterns, if not necessarily speciation, in Amazonian forest organisms. These results highlight the potential of nonforest species to contribute to an understanding of the history of the Amazonian rainforests themselves.

  9. Effects of Crotalus durissus collilineatus venom in the isolated rat kidney.

    Science.gov (United States)

    Amora, Daniela N; Sousa, Ticiana M; Martins, Alice M C; Barbosa, Paulo S F; Magalhães, Marta R; Toyama, Marcus H; Fonteles, Manassés C; de Menezes, Dalgimar B; Monteiro, Helena S A

    2006-03-01

    Ophidian accidents caused by the subspecies Crotalus durissus are responsible for high morbity and mortality rates. Acute renal failure is a common complication observed in these accidents. The aim of the present study was to investigate the renal effects promoted by the venom of C. d. collilineatus and its fractions, crotoxin and phospholipase A2. C. d. collilineatus (Cdc; 30 microg mL(-1)), crotoxin (CTX; 10 microg mL(-1)) and phospholipase A2 (PLA2; 10 microg mL(-1)) were tested in isolated rat kidney. The first 30 min of each experiment were used as an internal control and Cdc or its fractions, CTX and PLA2 were added to the system after this period. All experiments lasted 120 min. The venom of Cdc decreased perfusion pressure (PP; control120 = 110.3 +/- 3.69 mmHg; Cdc120 = 96.7+/-8.1 mmHg), renal vascular resistance (RVR; control120 = 6.42+/-0.78 mmHg mL g(-1) min(-1); Cdc120 = 4.8+/-0.56 mmHg/mL g(-1) min(-1)), urinary flow (UF; control120 = 0.19+/-0.03 mL g(-1) min(-1); Cdc120 = 0.12 +/- 0.01 mL g(-1) min(-1)), and glomerular filtration rate (GFR; control120 = 0.79 +/- 0.07 mL g(-1) min(-1); Cdc120 = 0.53 +/- 0.09 mL g(-1) min(-1)), but had no effect on the percent of sodium tubular transport (%TNa+), percent of chloride tubular transport (%TK+) and percent of potassium tubular transport (%TCl-). CTX and PLA2 reduced the GFR, while UF, PP and RVR remained stable during the full 120 min of perfusion. Crotoxin administration also diminished the %TK+ (control120 = 69.94 +/- 6.49; CTX120 = 33.28 +/- 4.78) and %TCl- (control120 = 79.53 +/- 2.67; CTX120 = 64.62 +/- 6.93). PLA2 reduced the %TK+, but exerted no effect on the %TNa+ or on that of TCl-. In conclusion, the C. d. collilineatus venom altered the renal functional parameters evaluated. We suggest that crotoxin and phospholipase A2 were involved in this process, since the renal effects observed would be due to the synergistic action of the components of the venom.

  10. Renal and vascular effects of Crotalus durissus cumanensis venom and its crotoxin fraction

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    TP Pereira

    2011-01-01

    Full Text Available In this study, we evaluated the actions of Crotalus durissus cumanensis venom (CDCmV, and its crotoxin (Crtx fraction, on renal and vascular functions in Wistar rats. In isolated perfused kidneys, CDCmV (10 µg/mL significantly increased the perfusion pressure (PP from 110.7 ± 2.4 to 125.3 ± 2.8 mmHg after 30 minutes. This effect was accompanied by an increased renal vascular resistance (RVR from 5.4 ± 0.1 to 6.2 ± 0.2 mmHg/mL.g-1.min-1. We observed decreases in urinary flow (UF from 0.13 ± 0.01 to 0.05 ± 001 mL.g-1.min-1 and glomerular filtration rate (GFR from 0.66 ± 0.06 to 0.18 ± 0.02 mL.g-1.min-1. Crtx did not change PP or RVR, but diminished GFR (from 0.65 ± 0.05 to 0.26 ± 003 mL.g-1.min-1 and UF (from 0.11 ± 0.008 to 0.09 ± 0.008 mL.g-1.min-1. Both CDCmV and Crtx reduced the percentage of tubular transport of sodium, chloride and potassium. The cytotoxicity of these substances against MDCK cells was tested by the MTT method: only CDCmV caused a decrease in the cell viability with an IC50 of 5.4 µg/mL. In endothelium-intact isolated aortic rings, CDCmV (0.1 to 30 µg/mL increased the sustained phenylephrine-induced contraction to a value of 130.0 ± 6.6% of its corresponding control, but showed a relaxant effect in endothelium-denuded preparations. Similar results were observed in aortic rings contracted with potassium (40 mM. Crtx was ineffective in aortic ring assays. Thus, it is reasonable to suggest that the renal effects induced by the CDCmV may be due to its influence on the endothelium's ability to release factors that can alter the contractile behavior of vascular smooth muscle. In conclusion, CDCmV is toxic to kidney cells. It changes parameters of the renal function including the glomerular filtration rate, renal vascular resistance and tubular transport. The actions induced by CDCmV also involve endothelium-dependent vasoactive properties. Their effects may be only partially attributed to Crtx.

  11. Biochemical and biological characterization of two serine proteinases from Colombian Crotalus durissus cumanensis snake venom.

    Science.gov (United States)

    Patiño, Arley Camilo; Pereañez, Jaime Andrés; Gutiérrez, José María; Rucavado, Alexandra

    2013-03-01

    Two clotting serine proteinases, named Cdc SI and Cdc SII, were isolated and characterized for the first time from Colombian Crotalus durissus cumanensis snake venom. The enzymes were purified using two chromatographic steps: molecular exclusion on Sephacryl S-200 and RP-HPLC on C8 Column. The molecular masses of the proteins, determined by MALDI-TOF mass spectrometry, were 28,561.4 and 28,799.2 Da for Cdc SI and Cdc SII, respectively. The aim of the present study was to evaluate enzymatic, coagulant and toxic properties of the two enzymes. The serine proteinases hydrolyzed specific chromogenic substrate (BaPNA) and exhibited a Michaelis-Menten behavior. Cdc SI had V(max) of 0.038 ± 0.003 nmol/min and K(M) of 0.034 ± 0.017 mM, while Cdc SII displayed values of V(max) of 0.267 ± 0.011 nmol/min and K(M) of 0.145 ± 0.023 mM. N-terminal sequences were VIGGDEXNIN and VIGGDICNINEHNFLVALYE for Cdc SI and Cdc SII, respectively. Molecular masses, N-terminal sequences, inhibition assays, and enzymatic profile suggest that Cdc SI and Cdc SII belong to the family of snake venom thrombin-like enzymes. These serine proteinases differed in their clotting activity on human plasma, showing a minimum coagulant dose of 25 μg and 0.571 μg for Cdc SI and Cdc SII, respectively. Enzymes also showed coagulant activity on bovine fibrinogen and degraded chain α of this protein. Toxins lack hemorrhagic and myotoxic activities, but are capable to induce defibrin(ogen)ation, moderate edema, and an increase in vascular permeability. These serine proteinases may contribute indirectly to the local hemorrhage induced by metalloproteinases, by causing blood clotting disturbances, and might also contribute to cardiovascular alterations characteristic of patients envenomed by C. d. cumanensis in Colombia.

  12. Qualidade de medicamentos isentos de prescrição: um estudo com marcas de dipirona comercializadas em uma drogaria de Cascavel (PR, Brasil Quality of over-the-counter medicines: a study with dipyrone brands commercialized in a drugstore in Cascavel city (Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    André Leandro Knappmann

    2010-11-01

    Full Text Available A dipirona é um fármaco muito utilizado pela população brasileira. É considerado seguro mesmo em gestantes, lactentes e crianças, mas é proibido em alguns países do mundo pelo suposto papel de causar agranulocitose e anemia aplástica. Em 2001, a Agência Nacional de Vigilância Sanitária (Anvisa considerou que os medicamentos com esse fármaco apresentavam bom risco-benefício em relação a outros de indicação semelhante. Porém, de nada adianta segurança no uso de um medicamento se este é de baixa qualidade. É comum encontrar no mercado brasileiro medicamentos fora dos padrões, o que se constitui em um risco para a população em geral. Dessa forma, foram analisadas sete amostras de marcas diferentes de dipirona solução oral comercializados em uma farmácia de Cascavel (PR. Os resultados demonstram que a fiscalização quanto à qualidade de medicamentos similares precisa ser aprimorada, pois eles foram os que mais apresentaram desvios de qualidade.Dipyrone is an antipyretic and analgesic medicine very used by the Brazilian population. The administration is considered safe even in pregnant women, nurseling and children, but is forbidden in some countries, as supposedly causes agranulocytosis and aplastic anemia. In 2001, National Health Surveillance Agency (Anvisa approved the commercialization of this medicine in Brazil. However, it does not matter the safeness in the use of a medicine advances, if it does not have quality. Based on this quality, this work was elaborated, that analyzes seven samples of commercialized different marks of dipyrone oral solution in pharmaceutical establishment in the Cascavel city, Paraná, Brazil. The results demonstrate that the quality control of similar drugs must be improved as were the ones that presented quality deviations.

  13. Assessing the influence of mechanical ventilation on blood gases and blood pressure in rattlesnakes

    DEFF Research Database (Denmark)

    Bertelsen, Mads F.; Buchanan, Rasmus; Jensen, Heidi M.

    2015-01-01

    , randomized trial. ANIMALS: Twenty one fasted adult South American rattlesnakes (Crotalus durissus terrificus). METHODS: Snakes were anesthetized with propofol (15 mg kg(-1) ) intravenously, endotracheally intubated and assigned to one of four ventilation regimens: Spontaneous ventilation, or mechanical...

  14. Antropometria, maturação sexual e idade da menarca de acordo com o nível socioeconômico de meninas escolares de Cascavel (PR Antropometry, sexual maturation and menarcheal age according to socioeconomic status of schoolgirls from Cascavel (PR

    Directory of Open Access Journals (Sweden)

    Everton Paulo Roman

    2009-01-01

    Full Text Available OBJETIVO: Avaliar a idade da menarca e a antropometria de meninas escolares de diferentes níveis socioeconômicos em Cascavel (Estado do Paraná -Região Sul do Brasil. MÉTODOS: Estudo transversal realizado no ano de 2006 com 2.761 meninas em 27 escolas públicas e particulares. A idade da menarca foi obtida pelo método status quo. Para a avaliação da maturação sexual foi realizada a autoavaliação de acordo com os critérios descritos por Tanner. O nível socioeconômico foi obtido por meio do questionário da ABEP (2003. Avaliou-se o peso e a estatura, obtendo-se o índice de massa corporal (IMC. Foram utilizados a análise estatística descritiva e o teste de Mann-Whitney, com nível de significância de 5%. RESULTADOS: A idade da menarca geral foi 12,2±1,2 anos e 12,1±1,1; 12,3±1,2 e 12,4±1,2 anos para os tercis alto, médio e baixo de nível socioeconômico, respectivamente. Foram constatados valores superiores para as meninas pós-menarca no peso e na estatura dos 10 aos 17 anos de idade e no IMC dos 11 aos 14 anos de idade. CONCLUSÕES: As meninas de nível socioeconômico alto apresentaram idade da menarca em idade inferior aos demais níveis socioeconômicos. Embora estas diferenças tenham sido observadas em níveis socioeconômicos distintos, biologicamente foram consideradas pequenas.OBJECTIVE: To evaluate age of menarche and anthropometric data of schoolgirls of different socioeconomic levels in Cascavel, state of Parana, in Southern Brazil. METHODS: A cross-sectional study was carried out in 2006 with 2,761 girls evaluated in 27 private and public schools. Age at menarche was obtained by the status quo method. Sexual maturity was assessed by self evaluation, according to Tanner's criteria. Socioeconomic level was obtained through the ABEP (2003 questionnaire. Data of weight, stature and Body Mass Index (BMI were obtained. Data were analyzed by descriptive statistics and Mann-Whitney test at 5% significance. RESULTS: Age

  15. Formas de uso medicinal da babosa e camomila pela população urbana de Cascavel, Estado do Paraná - doi: 10.4025/actascihealthsci.v32i2.6446 Medicinal use of the Aloe and Chamomile for the urban population of Cascavel, Paraná State - doi: 10.4025/actascihealthsci.v32i2.6446

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    Claudia Tatiana Araujo Cruz-Silva

    2010-09-01

    Full Text Available A etnobotânica se caracteriza por buscar entender a relação entre as plantas e o homem, podendo-se através desta conhecer a utilização das plantas medicinais como forma de tratamento. O objetivo deste trabalho foi realizar um levantamento sobre as formas de utilização da Babosa (Aloe vera L. e da Camomila (Matricaria chamomilla L.. A coleta de dados envolveu entrevistas de forma aleatória a 400 pessoas, através de um questionário semi-estruturado, no município de Cascavel, Estado do Paraná. Observou-se que 65% da população utilizam à babosa ou a camomila na cura ou alívio de doenças. A maioria dos entrevistados possui renda de 2 a 4 salários mínimos (60,25%, idade entre 28 a 38 anos (30,75%, tendo concluído o ensino médio (33,75%. O principal motivo pelo qual a população se utiliza de plantas medicinais é por ser natural (71,84%. A forma de preparo mais freqüente da camomila foi por infusão (63,38%, utilizando as flores (92%. Para a babosa 100% utilizam suas folhas na forma de cataplasma (43,88%. Apenas 3% da população relataram ter apresentado algum tipo de reação adversa, durante o período de utilização. Conclui-se que o uso destas plantas pela população é freqüente, sendo um recurso adicional ao uso de medicamentos.The ethnobotany, which is characterized by look for to understand the relationship between the plants and the man, being been able through this to know the use of the medicinal plants as treatment. The objective of this work was to accomplish a rising on the forms of use of the Aloe (Aloe vera L. and of the Chamomile (Matricaria chamomilla L.. The collection of data involved interviews in a random way to a sample of 400 people through a semi-structured questionnaire, in the city of Cascavel, Paraná State. It was observed that 65% of the population use to aloe or the chamomile in the cure or relief of diseases. Most of the interviewees possesses income from 2 to 4 minimum wages (60.25%, age

  16. Estudo sobre a evasão de pacientes em psicoterapia oferecida pela clínica escola da Unipan/Anhanguera de Cascavel-PR por membros da comunidade de baixa renda

    OpenAIRE

    Siqueira, Vinicius Reis de; Unipan - Anhanguera

    2013-01-01

    Considerando-se a importância da psicoterapia individual para os psicólogos, e o fato de existir um número consideravelmente alto de desistências da psicoterapia por pessoas que possuem uma baixa renda, este estudo buscou verificar os possíveis motivos que levam a evasão desta população na clinica escola da UNIPAN/Anhanguera de Cascavel/PR. Para tanto houve um processo de seleção dos atendimentos realizados no ano de 2010 para alcançar esta população (de baixa renda que desistiu em até 7 enco...

  17. Irradiação global, direta e difusa, para a região de Cascavel, Estado do Paraná - DOI: 10.4025/actascitechnol.v28i1.1288

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    Patrícia Aparecida de Oliveira Drechmer

    2006-03-01

    Full Text Available O presente trabalho apresenta um estudo sobre os níveis das irradiações global, direta e difusa incidentes na superfície terrestre, a irradiação incidente no topo da atmosfera e a insolação relativa mensal, para a cidade de Cascavel-PR. As componentes da radiação solar foram monitoradas entre janeiro de 2001 e fevereiro de 2003 na Estação Experimental Agrometeorológica da Unioeste, Cascavel, Estado do Paraná. Na medição da radiação global foi utilizado um piranômetro KIPP & ZONEN-CM3 e para medir a componente direta foi utilizado um pireliômetro EPPLEY-NIP acoplado a um rastreador solar. A radiação difusa foi calculada pela diferença entre as radiações global e direta. Os níveis médios mensais das irradiações global, direta e difusa, foram respectivamente, 51,8%, 31,7% e 20,17% da irradiação incidente no topo da atmosfera. O índice de insolação relativa apresentou valor máximo em agosto (61,43% e mínimo em fevereiro (34,35%, períodos caracterizados respectivamente por dias de céu limpo e nublados no hemisfério sul

  18. Influência da temperatura corporal de cascavéis (Crotalus durissus submetidas à anestesia com cetamina Influence of body temperature on rattlesnakes (Crotalus durissus anesthetized with ketamine

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    Adriano B. Carregaro

    2009-12-01

    Full Text Available O estudo objetivou verificar a influência da temperatura corporal nos parâmetros fisiológicos e nos períodos de indução e recuperação anestésicos de cascavéis (Crotalus durissus anestesiadas com cetamina. Os animais foram previamente submetidos à hipotermia (HIPO (The aim of the study was to verify the influence of the body temperature under physiological values and latency and recovery times on rattlesnakes anesthetized with ketamine. The animals were previously submitted to hypothermia (HYPO (<22°C and normothermia (30°C (NORMO and then, anesthetized with 80 mg/kg IM of ketamine. Latency and recovery times were evaluated by head tonus, muscular tonus and righting reflex. Heart rate (HR, time of apnea and body temperature were measured before and 5, 10, 15, 30, 60, 90 and 120 minutes after ketamine administration. Blood gases parameters were measured before, 30 and 60 minutes. It was not observed difference on latency time in both groups. The recovery time was higher on HYPO (5,5 hours compared to NORMO (3,5 hours. HR was higher on NORMO compared to HYPO. Time of apnea was the same pattern on both groups. Compared to basal levels, time of apnea was shorter between 5 to 30 min on both groups. Respiratory acidosis was observed only at 0 min in NORMO. SvO2 was higher after 30 min, the same as with PvO2 in both groups. PvCO2 reduced after 30 min in both groups. It was evident that body temperature exerts intense influence on the recovery time on rattlesnakes anesthetized with ketamine.

  19. EFECTO DEL AZUL DE METILENO EN LA HIPOTENSIÓN Y BRADICARDIA ASOCIADAS A LA ADMINISTRACIÓN DE UNA DOSIS LETAL DEL VENENO TOTAL DE CASCABEL VENEZOLANA (Crotalus durissus cumanensis) EN RATAS

    DEFF Research Database (Denmark)

    Briceno, Elena; Zerpa, Hector; Ascanio, Elias

    2010-01-01

    The effects of pre treatment with methylene blue (MB) on the cardiovascular effect caused by the administration of total venom (TV) of Crotalus durissus cumanensis was studied in adult rats, allocated into two groups: group I, six rats treated with TV (single dose: 1.5 mg/kg, IV) and group II, si...

  20. EFECTO DEL AZUL DE METILENO EN LA HIPOTENSIÓN Y BRADICARDIA ASOCIADAS A LA ADMINISTRACIÓN DE UNA DOSIS LETAL DEL VENENO TOTAL DE CASCABEL VENEZOLANA (Crotalus durissus cumanensis) EN RATAS

    DEFF Research Database (Denmark)

    Briceno, Elena; Zerpa, Hector; Ascanio, Elias

    2010-01-01

    The effects of pre treatment with methylene blue (MB) on the cardiovascular effect caused by the administration of total venom (TV) of Crotalus durissus cumanensis was studied in adult rats, allocated into two groups: group I, six rats treated with TV (single dose: 1.5 mg/kg, IV) and group II, si...

  1. Espaço urbano e criminalidade violenta: análise da distribuição espacial dos homicídios no município de Cascavel/PR

    Directory of Open Access Journals (Sweden)

    Fernanda Pamplona Ramão

    2010-02-01

    Full Text Available A pesquisa buscou examinar e compreender a incidência desigual dos homicídios no perímetro urbano da cidade de Cascavel, no Paraná, entre os anos 2000 e 2006. Procurou-se entender porque esse tipo de crime variou de uma unidade administrativa para outra e a relação existente entre esse fenômeno e desigualdades socioeconômicas, de infra-estrutura e serviços urbanos. Partiu-se da discussão sobre o processo de urbanização desordenada no Brasil, que favoreceu a emergência e/ou a intensificação de diversas problemáticas, dentre as quais a da criminalidade violenta e a dos homicídios. A cidade de Cascavel apresenta altas taxas de homicídio, sendo que poucas unidades administrativas as concentram. Nesse contexto e com base nas explicações postuladas na literatura de referência, realizou-se uma pesquisa empírica sobre a incidência desigual dos homicídios no referido município e sua associação com indicadores de desigualdade. Utilizou-se a técnica estatística de Análise Exploratória de Dados Espaciais (AEDE, além de uma análise de sobreposição espacial de informações. Constatou-se que o fenômeno em estudo apresentou forte associação com as desigualdades ao longo do espaço urbano. Pode-se afirmar, portanto, que os cenários de "sobreposição de carências", conseqüentemente de baixa qualidade de vida da população residente, potencializaram a ocorrência de conflitos violentos que resultaram em morte.

  2. Avaliação da resposta inflamatória hematológica em cascavéis (Crotalus durissus Linnaeus, 1758 inoculadas com BCG Assessment of blood inflammatory response in BCG stimulated rattlesnakes (Crotalus durissus Linnaeus, 1758

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    Wellington Bandeira da Silva

    2009-12-01

    Full Text Available A criação de serpentes peçonhentas em cativeiro para produção de soros antipeçonhas possui crescente importância para a saúde pública devido ao aumento do número de notificações de acidentes ofídicos a cada ano no Brasil. Iniciado no século XX, ainda hoje essa atividade apresenta alguns desafios como a instalação de doenças no plantel. O hemograma é um exame de triagem clínica que auxilia no diagnóstico de diversas moléstias que acometem diferentes espécies de animais, no entanto ainda pouco estudado em serpentes. A caracterização das alterações hematológicas em cascavéis inoculadas experimentalmente com BCG pode servir de base na utilização deste exame no auxílio ao diagnóstico de infecções bacterianas na espécie. Dessa forma, foram realizados exames hematológicos em 10 serpentes da espécie Crotalus durissus pertencentes ao plantel da Divisão de Herpetologia do Instituto Vital Brazil. Os animais foram divididos em dois grupos (Grupos 1 e 2, homogêneos entre si em relação ao peso e proporção sexual. Os dois grupos foram inoculados com BCG e submetidos à coleta de sangue antes da inoculação e em três momentos pós-inoculação (3º, 5º, e 7º dias para o Grupo 1 e 11º, 17º e 21º dias para o Grupo 2. O hemograma foi realizado por método semidireto pela utilização de líquido de Natt e Herrick e as lâminas foram coradas pelo Giemsa. Observou-se anemia discreta, com redução dos valores de concentração de hemoglobina corpuscular média e da hemoglobina globular média no Grupo 1 que foi relacionada à doença inflamatória. A trombocitopenia observada no Grupo 2 sugeriu a atuação deste tipo celular em processos inflamatórios. Um único animal do Grupo 1 apresentou granulocitose e alguns animais apresentaram discreta azurofilia. Observaram-se alterações morfológicas nos leucócitos. Os granulócitos apresentaram granulações grosseiras e os azurófilos apresentaram aumento de tamanho e

  3. Intraspecies differences in hemostatic venom activities of the South American rattlesnakes, Crotalus durissus cumanensis, as revealed by a range of protease inhibitors.

    Science.gov (United States)

    Salazar, Ana M; Aguilar, Irma; Guerrero, Belsy; Girón, María E; Lucena, Sara; Sánchez, Elda E; Rodríguez-Acosta, Alexis

    2008-09-01

    Crotalus durissus cumanensis is an endemic rattlesnake found in Venezuela and Colombia. In this study, a comparative analysis of hemorrhagic, coagulation and fibrino(geno)lytic activities in the presence or absence of protease inhibitors was performed with venoms of the same species Crotalus durissus cumanensis, from seven geographical regions of Venezuela (Lagunetica, Santa Teresa, Carrizales, Guarenas, Anzoátegui, Margarita and Maracay). Lagunetica, Carrizales and Anzoátegui venoms induced hemorrhagic activity. All venoms, except that of snakes from the Carrizales region presented thrombin-like activity, which was inhibited completely by phenylmethanesulfonyl fluoride and ethylene glycol-bis-N, N,N',N'-tetraacetic acid. This effect of the latter could be explained by the high chelant calcium effect, which is a cofactor for the fibrin polymerization process. Soybean trypsin inhibitor was effective on Santa Teresa venom. Antithrombin III/Hep complex and phenantroline partially inhibited this activity in all venoms except Margarita and Anzoátegui, respectively, which were not inhibited. Serine protease inhibitors were more effective against thrombin, kallikrein and plasmin-like amidolytic activities. Additionally, metalloprotease inhibitors significantly inhibited the t-PA-like amidolytic activity and completely the hemorrhagic and fibrino(geno)lytic activities. In conclusion, the thrombin-like activity observed in these venoms was partially reduced by serine protease inhibitors, indicating the possible presence of catalytic domains in those enzymes that do not interact with these inhibitors. Using protease inhibitors on venom hemostatic activities could contribute to our understanding of the active components of snake venom on the hemostatic system, and further reveal the intraspecies variation of venoms, which is important in the treatment of envenomation, and in addition represents an interesting model for the study of venom in hemostasis.

  4. Thalidomide and pentoxifylline block the renal effects of supernatants of macrophages activated with Crotalus durissus cascavella venom

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    A.M.C. Martins

    2004-10-01

    Full Text Available Because thalidomide and pentoxifylline inhibit the synthesis and release of tumor necrosis factor-alpha (TNF-alpha, we determined the effect of these drugs on the renal damage induced by supernatants of macrophages activated with Crotalus durissus cascavella venom in order to identify the role of TNF-alpha in the process. Rat peritoneal macrophages were collected with RPMI medium and stimulated in vitro with C.d. cascavella venom (10 µg/ml in the absence and presence of thalidomide (15 µM or pentoxifylline (500 µM for 1 h and washed and kept in culture for 2 h. Supernatant (1 ml was tested on an isolated perfused rat kidney (N = 6 for each group. The first 30 min of each experiment were used as control. The supernatant was added to the perfusion system. All experiments lasted 120 min. The toxic effect of the preparation of venom-stimulated macrophages on renal parameters was determined. At 120 min, thalidomide (Thalid and pentoxifylline (Ptx inhibited (P < 0.05 the increase in perfusion pressure caused by the venom (control = 114.0 ± 1.3; venom = 137.1 ± 1.5; Thalid = 121.0 ± 2.5; Ptx = 121.4 ± 4.0 mmHg, renal vascular resistance (control = 4.5 ± 0.2; venom = 7.3 ± 0.6; Thalid = 4.5 ± 0.9; Ptx = 4.8 ± 0.6 mmHg/ml g-1 min-1, urinary flow (control = 0.23 ± 0.001; venom = 0.44 ± 0.01; Thalid = 0.22 ± 0.007; Ptx = 0.21 ± 0.009 ml g-1 min-1, glomerular filtration rate (control = 0.72 ± 0.06; venom = 1.91 ± 0.11; Thalid = 0.75 ± 0.04; Ptx = 0.77 ± 0.05 ml g-1 min-1 and the decrease in percent tubular sodium transport (control = 77.0 ± 0.9; venom = 73.9 ± 0.66; Thalid = 76.6 ± 1.1; Ptx = 81.8 ± 2.0%, percent tubular chloride transport (control = 77.1 ± 1.2; venom = 71.4 ± 1.1; Thalid = 77.6 ± 1.7; Ptx = 76.8 ± 1.2%, and percent tubular potassium transport (control = 72.7 ± 1.1; venom = 63.0 ± 1.1; Thalid = 72.6 ± 1.0; Ptx = 74.8 ± 1.0%, 30 min before and during the stimulation of macrophages with C.d. cascavella venom

  5. Eficiência de um conjunto fotovoltaico em condições reais de trabalho na região de Cascavel - DOI: 10.4025/actascitechnol.v30i2.5496

    Directory of Open Access Journals (Sweden)

    Estor Gnoatto

    2008-10-01

    Full Text Available No presente trabalho, foi desenvolvido um estudo sobre um sistema fotovoltaico autônomo, com armazenamento de energia, instalado na Estação Experimental Agrometeorológica da Universidade Estadual do Oeste do Paraná, Campus Cascavel, a uma Latitude 24°59' Sul, Longitude de 53°26' Oeste, com altitude de 682 m, em condições reais de campo, durante o ano de 2002. Foi avaliada a eficiência dos painéis fotovoltaicos em condições reais de campo, sua curva característica foi determinada utilizando, na aquisição de dados, um micrologger da Campbell Scientific-Inc, modelo CR10X, programado para realizar uma leitura por segundo de cada canal e armazenar a média aritmética de 5 minutos dos dados das componentes de radiação solar global no plano horizontal e global na incidência do painel; temperatura ambiente e temperatura de operação do painel; tensão e corrente do sistema fotovoltaico. Os resultados mostram que a eficiência média mensal registrou valores na faixa de 8,65 a 9,17%, com uma média de 8,84%, com variação percentual entre o índice máximo e mínimo de 5,67%. Pode, assim, representar fornecimento médio mensal de energia de 5,57 kWh por painel.

  6. Avaliação da interação de compostos ativos hidratantes com modelo de biomembrana de Crotalus durissus por meio de calorimetria exploratória diferencial e espectroscopia RAMAN

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    MICHELE TAKAOKA

    2010-06-01

    Full Text Available O objetivo foi verificar as alterações no estrato córneo em modelos alternativos de membrana após a aplicação de ativos hidratantes envolvendo métodos biofísicos. O modelo de biomembrana utilizado foi a muda de pele de Crotalus durissus e os ativos hidratantes foram: uréia, silício orgânico, extrato vegetal de Imperata cylindrica, reação de xilitol e glicose e componentes de NMF. Os resultados da avaliação das alterações do modelo por meio de Espectroscopia Raman com Transformada de Fourier sugerem que os ativos hidratantes confirmam segurança necessária, pois não alteraram de forma acentuada a estrutura do estrato córneo. Utilizando-se Calorimetria Exploratória Diferencial pode-se indicar que a solução de silício orgânico e o gel hidrofílico com uréia apresentaram melhor poder hidratante. Palavras-chave: Estrato córneo. Hidratação. Métodos biofísicos. Crotalus durissus. FT-Raman, DSC. ABSTRACT Study of the interaction of moisturizers with a Crotalus durissus biomembrane by differential scanning calorimetry and RAMAN spectroscopy The objective of this research was to use biophysical techniques to investigate the alterations induced in a biomembrane model of the stratum corneum by the application of moisturizers. The biomembrane was obtained from the skin shed by the rattlesnake Crotalus durissus and the active moisturizing compounds were: urea, dimethylsilanol hyaluronate, Imperata cylindrical plant extract, carbohydrates and natural moisturizing factors (NMF components. Results from FT-Raman spectroscopy suggested that the moisturizers were safe, since they did not promote modifications in the structure of the stratum corneum. Differential scanning calorimetry results indicated that the solution containing the organic silicon compound and the gel with urea showed the best hydrating effects on the stratum corneum. Keywords: Stratum corneum. Hydration. Biophysical techniques. Crotalus durissus

  7. cDNA and deduced primary structure of basic phospholipase A2 with neurotoxic activity from the venom secretion of the Crotalus durissus collilineatus rattlesnake

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    F.H.R. Fagundes

    2010-03-01

    Full Text Available To illustrate the construction of precursor complementary DNAs, we isolated mRNAs from whole venom samples. After reverse transcription polymerase chain reaction (RT-PCR, we amplified the cDNA coding for a neurotoxic protein, phospholipase A2 D49 (PLA2 D49, from the venom of Crotalus durissus collilineatus (Cdc PLA2. The cDNA encoding Cdc PLA2 from whole venom was sequenced. The deduced amino acid sequence of this cDNA has high overall sequence identity with the group II PLA2 protein family. Cdc PLA2 has 14 cysteine residues capable of forming seven disulfide bonds that characterize this group of PLA2 enzymes. Cdc PLA2 was isolated using conventional Sephadex G75 column chromatography and reverse-phase high performance liquid chromatography (RP-HPLC. The molecular mass was estimated using matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF mass spectrometry. We tested the neuromuscular blocking activities on chick biventer cervicis neuromuscular tissue. Phylogenetic analysis of Cdc PLA2 showed the existence of two lines of N6-PLA2, denominated F24 and S24. Apparently, the sequences of the New World’s N6-F24-PLA2 are similar to those of the agkistrodotoxin from the Asian genus Gloydius. The sequences of N6-S24-PLA2 are similar to the sequence of trimucrotoxin from the genus Protobothrops, found in the Old World.

  8. Impactos ambientais decorrentes da ocupação de áreas reguladoras do aporte de areia: a planície Costeira da Caponga, município de Cascavel, litoral leste cearense.

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    Antonio Jeovah de Andrade Meireles

    2008-03-01

    Full Text Available Pour définir les impacts environnementaux associés à l'occupation humaine dans des secteurs caractérisés par l'apport de sédiments (bypass sur la plage, via des champ de dunes et l’estuaire, ont été réalisées des études géo-environnementales dans la plaine côtière du Caponga, en y incluant un promontoire, une bande littorale et l’embouchure du canal estuarien de même nom. Les résultats ont été représentés dans des modèles évolutifs, intégrés à partir de la définition des flux de matière et d'énergie qui transitent dans la zone côtière. Les conséquences de l'expansion urbaine sur le promontoire, les dunes, la plage et le canal estuarien ont été caractérisées afin de définir des mesures adaptées de planification et de gestion.Environmental impacts caused by the occupation of areas regulating the sand input to the coast line in the coastal plain of Caponga, Cascavel town, East Coast of Ceará. To determine the environmental impacts related to human occupation in areas characterized by the input of sediments (bypass to the coastal line, through dunes and estuary, we carried out geoenvironmental studies in the coastal plain of Caponga. They included sections represented by a headland, coastal line and river mouth of the estuarine channel of Caponga. The results were represented in integrated evolutionary models based on the definition of flows of matter and energy that occur in the coastal area. The effects of urban expansion on the headland, dunes, shore and estuarine channel were characterized so as to define adequate measures of planning and management.Para definir os impactos ambientais associados à ocupação humana em áreas caracterizadas pelo aporte de sedimentos (bypass para a faixa de praia, via campo de dunas e estuário, foram realizados estudos geoambientais na planície costeira da Caponga, abrangendo setores representados por um promontório, faixa litorânea e desembocadura do canal estuarino

  9. Comparison of the effect of Crotalus simus and Crotalus durissus ruruima venoms on the equine antibody response towards Bothrops asper venom: implications for the production of polyspecific snake antivenoms.

    Science.gov (United States)

    Dos-Santos, Maria Cristina; Arroyo, Cynthia; Solano, Sergio; Herrera, María; Villalta, Mauren; Segura, Alvaro; Estrada, Ricardo; Gutiérrez, José María; León, Guillermo

    2011-02-01

    Antivenoms are preparations of immunoglobulins purified from the plasma of animals immunized with snake venoms. Depending on the number of venoms used during the immunization, antivenoms can be monospecific (if venom from a single species is used) or polyspecific (if venoms from several species are used). In turn, polyspecific antivenoms can be prepared by purifying antibodies from the plasma of animals immunized with a mixture of venoms, or by mixing antibodies purified from the plasma of animals immunized separately with single venom. The suitability of these strategies to produce polyspecific antibothropic-crotalic antivenoms was assessed using as models the venoms of Bothrops asper, Crotalus simus and Crotalus durissus ruruima. It was demonstrated that, when used as co-immunogen, C. simus and C. durissus ruruima venoms exert a deleterious effect on the antibody response towards different components of B. asper venom and in the neutralization of hemorrhagic and coagulant effect of this venom when compared with a monospecific B. asper antivenom. Polyspecific antivenoms produced by purifying immunoglobulins from the plasma of animals immunized with venom mixtures showed higher antibody titers and neutralizing capacity than those produced by mixing antibodies purified from the plasma of animals immunized separately with single venom. Thus, despite the deleterious effect of Crotalus sp venoms on the immune response against B. asper venom, the use of venom mixtures is more effective than the immunization with separate venoms for the preparation of polyspecific bothropic-crotalic antivenoms. Copyright © 2010 Elsevier Ltd. All rights reserved.

  10. Arterial acid-base status during digestion and following vascular infusion of NaHCO(3) and HCl in the South American rattlesnake, Crotalus durissus.

    Science.gov (United States)

    Arvedsen, Sine K; Andersen, Johnnie B; Zaar, Morten; Andrade, Denis; Abe, Augusto S; Wang, Tobias

    2005-12-01

    Digestion is associated with gastric secretion that leads to an alkalinisation of the blood, termed the "alkaline tide". Numerous studies on different reptiles and amphibians show that while plasma bicarbonate concentration ([HCO(3)(-)](pl)) increases substantially during digestion, arterial pH (pHa) remains virtually unchanged, due to a concurrent rise in arterial PCO(2) (PaCO(2)) caused by a relative hypoventilation. This has led to the suggestion that postprandial amphibians and reptiles regulate pHa rather than PaCO(2). Here we characterize blood gases in the South American rattlesnake (Crotalus durissus) during digestion and following systemic infusions of NaHCO(3) and HCl in fasting animals to induce a metabolic alkalosis or acidosis in fasting animals. The magnitude of these acid-base disturbances were similar in magnitude to that mediated by digestion and exercise. Plasma [HCO(3)(-)] increased from 18.4+/-1.5 to 23.7+/-1.0 mmol L(-1) during digestion and was accompanied by a respiratory compensation where PaCO(2) increased from 13.0+/-0.7 to 19.1+/-1.4 mm Hg at 24 h. As a result, pHa decreased slightly, but were significantly below fasting levels 36 h into digestion. Infusion of NaHCO(3) (7 mmol kg(-1)) resulted in a 10 mmol L(-1) increase in plasma [HCO(3)(-)] within 1 h and was accompanied by a rapid elevation of pHa (from 7.58+/-0.01 to 7.78+/-0.02). PaCO(2), however, did not change following HCO(3)(-) infusion, which indicates a lack of respiratory compensation. Following infusion of HCl (4 mmol kg(-1)), plasma pHa decreased by 0.07 units and [HCO(3)(-)](pl) was reduced by 4.6 mmol L(-1) within the first 3 h. PaCO(2), however, was not affected and there was no evidence for respiratory compensation. Our data show that digesting rattlesnakes exhibit respiratory compensations to the alkaline tide, whereas artificially induced metabolic acid-base disturbances of same magnitude remain uncompensated. It seems difficult to envision that the central and

  11. Estabilidad de la actividad neutralizante del antiveneno ofídico conservado a 4°C y a 16°C contra el veneno de Cascabel (Crotalus durissus cumanensis de Colombia

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    Jaime Ramírez

    1995-12-01

    Full Text Available Se determinó la actividad neutralizante de la letalidad de varios lotes de antiveneno polivalente (no liofilizado, producidos por el Instituto Nacional de Salud, los cuales fueron retados contra una mezcla de venenos de cascabel (Crotalus durissus cumanensis de Colombia. Se compara la actividad de los diferentes lotes almacenados en oscuridad a las temperaturas de 4°C y 16°C. Se estableció el porcentaje remanente de la potencia de cada lote para cada temperatura en los diferentes años a lo largo del período del estudio. Se concluye que el antiveneno almacenando en las condiciones recomendadas, mantiene casi la totalidad de la capacidad de neutralización (97.9% luego de tres años de almacenamiento, lo quecorresponde a lafechade expiración del producto. En cambio, el antiveneno almacenado a 16°C y oscuridad, luego de dos años de almacenamiento, mantiene el 87.9% de su capacidad protectora contra veneno de cascabel, protección similara la encontrada bajo las mismas condiciones de almacenamiento, paraveneno de Taya X, pasados dos años de almacenamiento (l, sustentando la recomendación de descartar el producto, transcurridos dos años de almacenamiento en estas condiciones.

  12. Snakebites by Crotalus durissus ssp in children in Campinas, São Paulo, Brazil Acidentes por serpentes Crotalus durisssus ssp em crianças em Campinas, São Paulo, Brasil

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    Fábio BUCARETCHI

    2002-01-01

    Full Text Available From January, 1984 to March, 1999, 31 children under 15 y old (ages 1-14 y, median 8 y were admitted after being bitten by rattlesnakes (Crotalus durissus ssp. One patient was classified as "dry-bite", 3 as mild envenoming, 9 as moderate envenoming and 18 as severe envenoming. Most patients had neuromuscular manifestations, such as palpebral ptosis (27/31, myalgia (23/31 and weakness (20/31. Laboratory tests suggesting rhabdomyolysis included an increase in total blood creatine kinase (CK, 28/29 and lactate dehydrogenase (LDH, 25/25 levels and myoglobinuria (14/15. The main local signs and symptoms were slight edema (20/31 and erythema (19/31. Before antivenom (AV administration, blood coagulation disorders were observed in 20/25 children that received AV only at our hospital (incoagulable blood in 17/25. AV early reactions were observed in 20 of these 25 cases (9/9 patients not pretreated and 11/16 patients pretreated with hydrocortisone and histamine H1 and H2 antagonists. There were no significant differences in the frequency of patients with AV early reactions between the groups that were and were not pretreated (Fisher's exact test, p = 0.12. Patients admitted less than and more than 6 h after the bite showed the same risk of developing severe envenoming (Fisher's exact test, p = 1. No children of the first group (De janeiro de 1984 a março de 1999, 31 crianças com menos de 15 anos de idade (1 a 14 anos, mediana = 8 anos foram admitidas após terem sido picadas por Crotalus durissus ssp. Uma criança não apresentou manifestações clínicas de envenenamento, enquanto 3 foram classificadas como acidente leve, 9 como moderado e 18 como grave. A maioria das crianças apresentou envolvimento neuromuscular, tais como ptose palpebral (27/31, mialgia (23/31 e fraqueza (20/31. Alterações laboratoriais sugerindo rabdomiólise também foram observadas, como aumento das enzimas séricas CK (28/29 e LDH (25/25 e mioglobinúria (14/15. As

  13. A study on the venom yield of venomous snake species from Argentina.

    Science.gov (United States)

    de Roodt, A R; Dolab, J A; Galarce, P P; Gould, E; Litwin, S; Dokmetjian, J C; Segre, L; Vidal, J C

    1998-12-01

    A study on the venom yield of snakes from Argentina over a three year period was carried out on adult specimens of Bothrops alternatus (n = 74); Bothrops neuwiedii (n = 127); Bothrops ammodytoides (n = 30); Bothrops moojeni (n = 14); Bothrops jararaca (n = 14); B. jararacussu (n = 6); Crotalus durissus terrificus (n = 120) and Micrurus spp. (n = 6) as well as with 12 specimens of newborn C. d. terrificus kept in captivity. While for each species there was a positive correlation between venom yield and number of snakes milked, the correlation with the snake's body weights after individual milkings was even better, suggesting that the size of the snakes is more important in determining the venom yield than the number of snakes milked or the specimen's sex. Individual milkings indicated that, in addition to the snake size, when the amount of venom is normalized per 100 g body weight there is a species specific difference in venom yield. It follows the order B. jararacussu > B. moojeni approximately = B. jararaca approximately = B. alternatus > B. neuwiedii> Micrurus spp approximately = B. ammodytoides> C. d. terrificus. Although the venom yield per 100 g body weight of newborn C. d. terrificus specimens is 2-fold higher than that of adults, no correlation was observed between venom yield and body weight.

  14. Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin

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    G. Rádis-Baptista

    2005-12-01

    Full Text Available Snake venom (sv C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD. A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX, from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa and beta (CVXbeta , 12.6 kDa, joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria.

  15. Crotoxin in humans: analysis of the effects on extraocular and facial muscles Crotoxina em humanos: estudo da ação em músculos extraoculares e faciais

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    Geraldo de Barros Ribeiro

    2012-12-01

    Full Text Available PURPOSE: Crotoxin is the main neurotoxin of South American rattlesnake Crotalus durissus terrificus. The neurotoxic action is characterized by a presynaptic blockade. The purpose of this research is to assess the ability of crotoxin to induce temporary paralysis of extraocular and facial muscles in humans. METHODS: Doses of crotoxin used ranged from 2 to 5 units (U, each unit corresponding to one LD50. We first applied 2U of crotoxin in one of the extraocular muscles of 3 amaurotic individuals to be submitted to ocular evisceration. In the second stage, we applied crotoxin in 12 extraocular muscles of 9 patients with strabismic amblyopia. In the last stage, crotoxin was used in the treatment of blepharospasm in another 3 patients. RESULTS: No patient showed any systemic side effect or change in vision or any eye structure problem after the procedure. The only local side effects observed were slight conjunctival hyperemia, which recovered spontaneously. In 2 patients there was no change in ocular deviation after 2U crotoxin application. Limitation of the muscle action was observed in 8 of the 12 applications. The change in ocular deviation after application of 2U of crotoxin (9 injections was in average 15.7 prism diopters (PD. When the dose was 4U (2 applications the change was in average 37.5 PD and a single application of 5U produced a change of 16 PD in ocular deviation. This effect lasted from 1 to 3 months. Two of the 3 patients with blepharospasm had the hemifacial spasm improved with crotoxin, which returned after 2 months. CONCLUSIONS: This study provides data suggesting that crotoxin may be a useful new therapeutic option for the treatment of strabismus and blepharospasm. We expect that with further studies crotoxin could be an option for many other medical areas.OBJETIVO: A crotoxina é a principal neurotoxina da cascavel sul-americana Crotalus durissus terrificus e sua ação neurotóxica caracteriza-se por um bloqueio pr

  16. Aspectos clínico-patológicos e laboratoriais do envenenamento crotálico experimental em bovinos Clinical and pathological aspects of experimental Crotalus poisoning in cattle

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    Flávio A.S. Graça

    2008-06-01

    Full Text Available Reproduziu-se experimentalmente o envenenamento crotálico, através da inoculação, por via subcutânea, do veneno de Crotalus durissus terrificus (cascavel sul-americana em dez bovinos mestiços. Dois animais foram utilizados como controle. O bovino que recebeu dose de 0,03mg/kg de peso corporal, morreu 7h40min após a inoculação. A dose de 0,015mg/kg causou a morte em quatro de sete bovinos inoculados, enquanto os dois animais que receberam 0,0075mg/kg adoeceram discretamente e se recuperaram. Os sintomas tiveram início entre 1h30min e 13h45min após a inoculação. A evolução oscilou entre 5h25min e 45h para os animais que morreram e entre 33h15min e 17 dias entre os animais que se recuperaram. Os principais sinais nervosos observados foram diminuição da resposta aos estímulos externos, reflexos hipotônicos, arrastar dos cascos no solo, aparente apatia, paralisia do globo ocular e da língua, decúbito esternal e lateral. Verificaram-se também adipsia e, por vezes, petéquias nas mucosas vaginal e conjuntival. Houve discreto a moderado aumento do tempo de sangramento e moderado aumento do tempo de tromboplastina parcial ativada. Houve moderada leucocitose com neutrofilia, linfopenia relativa, eosinopenia, monocitose e discreto aumento do número de bastões. Foi evidenciado significativo aumento dos níveis séricos de creatinaquinase, contudo, não foram observadas alterações significativas através da urinálise. À necropsia constataram-se edema quase imperceptível no local da inoculação, discretas petéquias e sufusões no epicárdio, omento, vesícula biliar e mucosa da bexiga em alguns dos animais envenenados experimentalmente. Os exames histopatológicos revelaram necrose (hialinização de grupos de miócitos ou em miócitos isolados em dez diferentes músculos esqueléticos examinados, próximos ou distantes do local de inoculação em todos os animais necropsiados. Concluí-se que o envenenamento por Crotalus Sul

  17. Study of crotoxin on the induction of paralysis in extraocular muscle in animal model Estudo da crotoxina na indução de paralisia da musculatura extraocular em modelo animal

    Directory of Open Access Journals (Sweden)

    Geraldo de Barros Ribeiro

    2012-10-01

    crotoxina é a principal toxina do veneno da cobra cascavel sul-americana Crotalus durissus terrificus e causa bloqueio da neurotransmissão na junção neuromuscular. O objetivo deste estudo foi avaliar a ação e aplicabilidade da crotoxina na indução de paralisia da musculatura extrínseca ocular, e comparar seus efeitos com os da toxina botulínica do tipo A (TB-A. MÉTODOS: A crotoxina, com DL50 de 1,5 µg, foi aplicada no músculo reto superior direito de dez coelhos da raça neozelandesa, em concentrações que variaram de 0,015 µg a 150 µg. Em dois coelhos, utilizou-se 2 unidades de toxina botulínica do tipo A para análise comparativa. A avaliação da paralisia foi realizada através de eletromiografia seriada. Após a recuperação, que ocorreu em dois meses, seis coelhos foram sacrificados para estudo anátomopatológico. RESULTADOS: Os animais não apresentaram sinais de intoxicação sistêmica. Ptose palpebral transitória foi observada em quase todos os animais e permaneceu por até 14 dias. As toxinas causaram um bloqueio imediato da captação dos potenciais elétricos. A recuperação foi gradativa no período aproximado de um mês, observando-se sinais evidentes de regeneração no registro eletromiográfico. Os efeitos da crotoxina na paralização do músculo injetado foram proporcionais à concentração. A crotoxina, na concentração de 1,5 µg, induziu alterações semelhantes às da toxina botulínica do tipo A. Os achados anátomo-patológicos foram localizados somente na região em que se aplicou as toxinas, não havendo necrose de fibras musculares em nenhuma amostra analisada. As alterações causadas pela crotoxina também foram proporcionais à concentração utilizada e similares a toxina botulínica do tipo A na concentração de 1,5 µg. CONCLUSÃO: A crotoxina foi capaz de induzir paralisia transitória do músculo reto superior. Este efeito foi caracterizado pela redução na amplitude dos potenciais de ação e sinais inespec

  18. Crotamine and crotoxin interact with tumor cells and trigger cell death

    Energy Technology Data Exchange (ETDEWEB)

    Soares, Marcella Araugio; Pujatti, Priscilla Brunelli; Santos, Raquel Gouvea dos [Centro de Desenvolvimento da Tecnologia Nuclear CDTN/CNEN-MG, Belo Horizonte, MG (Brazil)]. E-mails: maso@cdtn.br; santosr@cdtn.br; Dias, Consuelo Latorre Fortes [Fundacao Ezequiel Dias FUNED, Belo Horizonte, MG (Brazil); Chavez Olortegui, Carlos Delfin [Universidade Federal de Minas Gerais UFMG, Belo Horizonte, MG (Brazil). Inst. de Ciencias Biologicas; Santos, Wagner Gouvea dos [Medical College of Virginia, Richmond, VA (United States). Neurosurgery Dept.

    2007-07-01

    Crotoxin (Crtx) and Crotamine (Crota) are polypeptides isolated from Crotalus durissus terrificus snake venom (CV). Previous reports have been shown therapeutic effects of Crotalus durissus terrificus venom and Crtx on skin, breast and lung tumours, although, the mechanisms of this antitumoral effect are still unknown. The aim of this work was to investigate the antitumoral effect of Crtx and Crota on brain tumours cells (GH3 and RT2) in vitro and their capacity of interaction with these tumour cells membranes. Cell survival after Crtx and Crota treatment was evaluated by MTT assay in different times post-treatment and apoptosis was evaluated by DAPI staining. In order to evaluate the specific interaction of Crtx and Crota, these polypeptides were radiolabelled, using {sup 125}I as radiotracer and binding assays were performed. The results were compared with the binding in nontumoral brain tissue. Crtx and Crota induced apoptosis on both tumour cells lineages but, Crota was more powerful than Crtx 90% and 20% cell death for RT2 cells; 80% and 20% cell death for GH3 cells, respectively). Both {sup 125}I-Crtx and {sup 125}I-Crota bound specifically in glioblastoma membranes. Nonetheless, CV polypeptides recognised glioblastoma cells with higher specificity than normal brain tissue. These results suggest that the Crtx and Crota interactions with the plasmatic membrane of tumour cells may be the first step of the cascade of signalling that trigger their antitumoral effect. (author)

  19. Prevalence of Hepatozoon spp. (Apicomplexa, Hepatozoidae among recently captured Brazilian snakes

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    O'Dwyer L.H.

    2003-01-01

    Full Text Available The goal of this study was to determine the prevalence of Hepatozoon spp. infection in recently captured snakes from Botucatu, São Paulo State, Brazil. Blood was collected from all snakes by ventral tail venipuncture. Blood smears were air dried, fixed with methanol, and stained with 10% Giemsa solution. The slides were microscopically examined for detection of hemoparasites by light microscopy at 250x magnification. A total of 238 snakes from 23 species were examined, of which 135 (56.7% were venomous and 103 (43.3% non-venomous snakes. The more numerous venomous species sampled were Crotalus durissus terrificus (n=108 and Bothrops jararaca (n=17 and non-venomous snakes were Oxyrhopus guibei (n=35, Boa constrictor amarali (n=18, and Waglerophis merremi (n=13. Hepatozoon spp. infection was detected in 39 (16.4% snakes. The prevalence in venomous and non-venomous snakes was 20.0% and 11.7%, respectively. The highest prevalences observed were 38.9% for Boa constrictor amarali, 35.3% for Bothrops jararaca, and 19.4% for Crotalus durissus terrificus.

  20. Envenomations by Bothrops and Crotalus snakes induce the release of mitochondrial alarmins.

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    Irene Zornetta

    Full Text Available Skeletal muscle necrosis is a common manifestation of viperid snakebite envenomations. Venoms from snakes of the genus Bothrops, such as that of B. asper, induce muscle tissue damage at the site of venom injection, provoking severe local pathology which often results in permanent sequelae. In contrast, the venom of the South American rattlesnake Crotalus durissus terrificus, induces a clinical picture of systemic myotoxicity, i.e., rhabdomyolysis, together with neurotoxicity. It is known that molecules released from damaged muscle might act as 'danger' signals. These are known as 'alarmins', and contribute to the inflammatory reaction by activating the innate immune system. Here we show that the venoms of B. asper and C. d. terrificus release the mitochondrial markers mtDNA (from the matrix and cytochrome c (Cyt c from the intermembrane space, from ex vivo mouse tibialis anterior muscles. Cyt c was released to a similar extent by the two venoms whereas B. asper venom induced the release of higher amounts of mtDNA, thus reflecting hitherto some differences in their pathological action on muscle mitochondria. At variance, injection of these venoms in mice resulted in a different time-course of mtDNA release, with B. asper venom inducing an early onset increment in plasma levels and C. d. terrificus venom provoking a delayed release. We suggest that the release of mitochondrial 'alarmins' might contribute to the local and systemic inflammatory events characteristic of snakebite envenomations.

  1. [Pharmacologic and enzymatic effects of snake venoms from Antioquia and Choco (Colombia)].

    Science.gov (United States)

    Otero, R; Guillermo Osorio, R; Valderrama, R; Augusto Giraldo, C

    1992-01-01

    We compared several pharmacological and enzymatic effects induced by 11 snake venoms from seven species, six of them from different geographic areas of Antioquia and Choco, north-west of Colombia, South America (Bothrops atrox, B. nasutus, B. schlegelii, B. punctatus, Lachesis muta, Micrurus mipartitus), and Crotalus durissus terrificus venom, from specimens captured in other provinces of the country (Tolima, Huila, Meta and Atlantico). Differences were observed in edema-forming, hemorrhage, defibrination, indirect hemolysis, myonecrosis, proteolysis and lethal activity between venoms from different genera or species, as well as according to the geographic area of origin in B. atrox and B. nasutus snake venoms. Bothrops venoms, in particular B. atrox and L. muta, produced major local effects. All of the venoms, including M. mipartitus, had myotoxic effects. The most defibrinating venoms were B. atrox, L. muta, B. punctatus and C. d. terrificus. All of the venoms had indirect hemolytic activity; the venom of M. mipartitus being greatest. The most lethal venoms were those of C. d. terrificus and M. mipartitus. Within Bothrops species, the venom of B. schlegelii was the least active in terms of local and systemic pathologic effects.

  2. Evaluation of snake venom phospholipase A{sub 2}: hydrolysis of non-natural esters

    Energy Technology Data Exchange (ETDEWEB)

    Pirolla, Renan A.S.; Baldasso, Paulo A.; Marangoni, Sergio; Moran, Paulo J.S.; Rodrigues, Jose Augusto R., E-mail: jaugusto@iqm.unicamp.b [University of Campinas (UNICAMP), SP (Brazil). Inst. of Chemistry. Dept. of Organic Chemistry

    2011-07-01

    Phospholipase A2 from the rattlesnake Crotalus durissus terrificus was employed for the first time to test its enantioselectivity on the hydrolysis of different non-natural esters. It was observed that the structure of this small enzyme is restrictive in the choice of its lipase action with non-natural substrates. Two forms of the enzyme were used; free and as its cross-linked enzyme aggregate (CLEA). With all substrates, the free enzyme showed activity similar to the CLEA preparation. The advantage of the CLEA phospholipase is the possibility to reuse it in several consecutive reactions without a decrease of activity and selectivity with good but higher yields and ee than with the free enzyme. (author)

  3. {sup 131}I-CRTX internal dosimetry: animal model and human extrapolation

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Henrique Martins de; Ferreira, Andrea Vidal; Soares, Marcella Araugio; Silveira, Marina Bicalho; Santos, Raquel Gouvea dos [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN-CNEN-MG), Belo Horizonte, MG (Brazil)], e-mail: hma@cdtn.br

    2009-07-01

    Snake venoms molecules have been shown to play a role not only in the survival and proliferation of tumor cells but also in the processes of tumor cell adhesion, migration and angiogenesis. {sup 125}I-Crtx, a radiolabeled version of a peptide derived from Crotalus durissus terrificus snake venom, specifically binds to tumor and triggers apoptotic signalling. At the present work, {sup 125}I-Crtx biokinetic data (evaluated in mice bearing Erlich tumor) were treated by MIRD formalism to perform Internal Dosimetry studies. Doses in several organs of mice were determinate, as well as in implanted tumor, for {sup 131}I-Crtx. Doses results obtained for animal model were extrapolated to humans assuming a similar concentration ratio among various tissues between mouse and human. In the extrapolation, it was used human organ masses from Cristy/Eckerman phantom. Both penetrating and non-penetrating radiation from {sup 131}I in the tissue were considered in dose calculations. (author)

  4. Effects of gamma radiation on snake venoms

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    Nascimento, N.; Spencer, P.J.; Andrade, H.F.; Guarnieri, M.C.; Rogero, J.R

    1998-06-01

    Ionizing radiation is able to detoxify several venoms, including snake venoms, without affecting significantly their immunogenic properties. In order to elucidate this phenomena, we conceived a comparative pharmacological study between native and irradiated (2,000 Gy) crotoxin, the main toxin of the South American rattlesnake Crotalus durissus terrificus. Crotoxin was isolated and purified by molecular exclusion chromatography, pI precipitation and, subsequently submitted to irradiation. Gel filtration of the irradiated toxin resulted in some high molecular weight aggregates formation. Crotoxin toxicity decreased two folds after irradiation, as determined by LD{sub 50} in mice. Native and irradiated crotoxin biodistribution ocurred in the same general manner, with renal elimination. However, in contrast to irradiated crotoxin, the native form was initially retained in kidneys. A later concentration (2-3 hr) appeared in phagocytic mononuclear cells rich organs (liver and spleen) and neural junction rich organs (muscle and brain)

  5. Comparative morphocytologic study of native and irradiated crotoxin on CBA/J mice tissues and cells; Estudo morfocitologico comparativo de crotoxina nativa e irradiada em tecidos e celulas de camundongos CBA/J

    Energy Technology Data Exchange (ETDEWEB)

    Cardi, Bruno A.

    1995-12-31

    Snake venoms submitted to gamma radiation are better immunogens, have less toxic activity and maintain immunogenic and antigenic properties, favoring its use in anti-sera production which is the main therapy in human accidents. Severe human accidents attributed to neurotoxicity, are caused by Crotalus durissus terrificus, which main toxin - crotoxin - acts on neuro muscular junctions, as detected by electrophysiological studies and could be detoxified by gamma radiation. In order to evaluate the biological effects of this irradiated protein, we analyzed, at morphological and immunohistochemical levels, organs and cells from CBA/J mice challenged with crude venom or purified crotoxin, in an acute intoxication model. Both toxins were submitted to 2 kGy irradiation with a Co-60 source, in water solution. (author). 140 refs., 10 figs., 1 tab.

  6. Effects of gamma radiation on snake venoms

    Science.gov (United States)

    Nascimento, N.; Spencer, P. J.; Andrade, H. F.; Guarnieri, M. C.; Rogero, J. R.

    1998-06-01

    Ionizing radiation is able to detoxify several venoms, including snake venoms, without affecting significantly their immunogenic properties. Inn order to elucidate this phenomena, we conceived a comparative pharmacological study between native and irradiated (2,000 Gy) crotoxin, the main toxin of the South American rattlesnake Crotalus durissus terrificus. Crotoxin was isolated and purified by molecular exclusion chromatography, pI precipitation and, susbequentely submitted to irradiaiton. Gel filtration of the irradiated toxin resulted in some high molecular weight aggregates formation. Crotoxin toxicity decreased two folds after irradiation, as determined by LD 50 in mice. Native and irradiated crotoxin biodistribution ocured in the same general manner, with renal elimination. However, in contrast to irradiated crotoxin, the native form was initially retained in kidneys. A later concentration (2-3 hr) appeared in phagocytic mononuclear cells rich organs (liver and spleen) and neural junction rich organs (muscle and brain).

  7. Acidente crotálico na infância: aspectos clínicos, laboratoriais, epidemiológicos e abordagem terapêutica

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    Palmira Cupo

    1991-06-01

    Full Text Available Foram atendidas no Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto/USP, durante os anos de 1980 a 1989, 21 crianças vítimas de acidente crotálico, 16 das quais apresentaram sinais clínicos de envenenamento grave à admissão e 5, de envenenamento moderado. Quatro pacientes (20% desenvolveram necrose tubular aguda, dois dos quais necessitaram métodos dialíticos. Um paciente (5% foi a óbito no 13.° dia do acidente, após intercorrências graves, como hemorragia digestiva e insuficiência respiratória aguda. São apresentadas as características clínicas, laboratoriais e epidemiológicas do acidente crotálico nesses pacientes, enfatizando os conhecimentos atuais sobre a fisiopatologia do envenenamento por Crotalus durissus terrificus. Ao mesmo tempo, pretendeu-se contribuir para a correta abordagem terapêutica, no que diz respeito ao emprego adequado do soro antiveneno e à prevenção da necrose tubular aguda, a mais séria e grave complicação do acidente crotálico.At the Hospital of Clinics of the Faculty of Medicine of Ribeirão Preto/USP during the years 1980-1989 21 children have been attended after rattlesnake bite: 16 severe and 5 with moderate envenomation. Four (20% developed acute tubular necrosis 2 necessitating dialysis. One patient died 13 days after the bite and grave complications including digestive hemorrhage and acute respiratory insuficiency. All patients preserved clinical laboratory and epidemiological characteristics of Crotalus durissus terrificus envenomation. We also comment on the correct management of such patients specially related to antivenin dosage and the prevention ofacute tubular the most serious complication of such an accident.

  8. A new heterologous fibrin sealant as a scaffold to cartilage repair-Experimental study and preliminary results.

    Science.gov (United States)

    de Barros, Caio Nunes; Miluzzi Yamada, Ana Lúcia; Junior, Rui Seabra F; Barraviera, Benedito; Hussni, Carlos Alberto; de Souza, Jaqueline Brandão; Watanabe, Marcos Jun; Rodrigues, Celso Antônio; Garcia Alves, Ana Liz

    2016-07-01

    Autologous fibrin gel is commonly used as a scaffold for filling defects in articular cartilage. This biomaterial can also be used as a sealant to control small hemorrhages and is especially helpful in situations where tissue reparation capacity is limited. In particular, fibrin can act as a scaffold for various cell types because it can accommodate cell migration, differentiation, and proliferation. Despite knowledge of the advantages of this biomaterial and mastery of the techniques required for its application, the durability of several types of sealant at the site of injury remains questionable. Due to the importance of such data for evaluating the quality and efficiency of fibrin gel formulations on its use as a scaffold, this study sought to analyze the heterologous fibrin sealant developed from the venom of Crotalus durissus terrificus using studies in ovine experimental models. The fibrin gel developed from the venom of this snake was shown to act as a safe, stable, and durable scaffold for up to seven days, without causing adverse side effects. Fibrin gel produced from the venom of the Crotalus durissus terrificus snake possesses many clinical and surgical uses. It presents the potential to be used as a biomaterial to help repair skin lesions or control bleeding, and it may also be used as a scaffold when applied together with various cell types. The intralesional use of the fibrin gel from the venom of this snake may improve surgical and clinical treatments in addition to being inexpensive and adequately consistent, durable, and stable. The new heterologous fibrin sealant is a scaffold candidate to cartilage repair in this study.

  9. A new heterologous fibrin sealant as a scaffold to cartilage repair—Experimental study and preliminary results

    Science.gov (United States)

    de Barros, Caio Nunes; Miluzzi Yamada, Ana Lúcia; Junior, Rui Seabra F; Barraviera, Benedito; Hussni, Carlos Alberto; de Souza, Jaqueline Brandão; Watanabe, Marcos Jun; Rodrigues, Celso Antônio

    2015-01-01

    Autologous fibrin gel is commonly used as a scaffold for filling defects in articular cartilage. This biomaterial can also be used as a sealant to control small hemorrhages and is especially helpful in situations where tissue reparation capacity is limited. In particular, fibrin can act as a scaffold for various cell types because it can accommodate cell migration, differentiation, and proliferation. Despite knowledge of the advantages of this biomaterial and mastery of the techniques required for its application, the durability of several types of sealant at the site of injury remains questionable. Due to the importance of such data for evaluating the quality and efficiency of fibrin gel formulations on its use as a scaffold, this study sought to analyze the heterologous fibrin sealant developed from the venom of Crotalus durissus terrificus using studies in ovine experimental models. The fibrin gel developed from the venom of this snake was shown to act as a safe, stable, and durable scaffold for up to seven days, without causing adverse side effects. Fibrin gel produced from the venom of the Crotalus durissus terrificus snake possesses many clinical and surgical uses. It presents the potential to be used as a biomaterial to help repair skin lesions or control bleeding, and it may also be used as a scaffold when applied together with various cell types. The intralesional use of the fibrin gel from the venom of this snake may improve surgical and clinical treatments in addition to being inexpensive and adequately consistent, durable, and stable. The new heterologous fibrin sealant is a scaffold candidate to cartilage repair in this study. PMID:26264444

  10. Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine.

    Science.gov (United States)

    Teixeira-Araújo, Ricardo; Castanheira, Patrícia; Brazil-Más, Leonora; Pontes, Francisco; Leitão de Araújo, Moema; Machado Alves, Maria Lucia; Zingali, Russolina Benedeta; Correa-Netto, Carlos

    2017-01-01

    Snakebite treatment requires administration of an appropriate antivenom that should contain antibodies capable of neutralizing the venom. To achieve this goal, antivenom production must start from a suitable immunization protocol and proper venom mixtures. In Brazil, antivenom against South American rattlesnake (Crotalus durissus terrificus) bites is produced by public institutions based on the guidelines defined by the regulatory agency of the Brazilian Ministry of Health, ANVISA. However, each institution uses its own mixture of rattlesnake venom antigens. Previous works have shown that crotamine, a toxin found in Crolatus durissus venom, shows marked individual and populational variation. In addition, serum produced from crotamine-negative venoms fails to recognize this molecule. In this work, we used an antivenomics approach to assess the cross-reactivity of crotalic antivenom manufactured by IVB towards crotamine-negative venom and a mixture of crotamine-negative/crotamine-positive venoms. We show that the venom mixture containing 20% crotamine and 57% crotoxin produced a strong immunogenic response in horses. Antivenom raised against this venom mixture reacted with most venom components including crotamine and crotoxin, in contrast to the antivenom raised against crotamine-negative venom. These results indicate that venomic databases and antivenomics analysis provide a useful approach for choosing the better venom mixture for antibody production and for the subsequent screening of antivenom cross-reactivity with relevant snake venom components.

  11. A taxonomic bibliography of the South American snakes of the Crotalus durissus complex (Serpentes, Viperidae

    Directory of Open Access Journals (Sweden)

    VANZOLINI PAULO E.

    2002-01-01

    Full Text Available A survey is made of the taxonomic literature on South American rattlesnakes (genus Crotalus, family Viperidae. Two main areas are emphasized: the attribution of the names proposed in the eighteenth century by Linnaeus and Laurenti, and the current scheme of division in subspecies. The attribution of names is examined based on the original descriptions and on relevant previous and contemporary literature. The presently adopted scheme, proposed by Klauber (1941, 1972 is found not entirely satisfactory, but reasonable enough - besides being hallowed by use. The scheme of geographical differentiation, intrinsically important and with broad practical implications (differentiation of the venom is found to be the culmination of a long series of deficient analyses, and in urgent need of proper investigation.

  12. Reactividad inmunoquímica de sueros anti- Caiman yacare y Caiman latirostris frente a sueros de diferentes especies

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    de Roodt, Adolfo Rafael

    2010-06-01

    Full Text Available Se estudió la reactividad inmunoquímica entre los sueros de distintas especies de reptiles frente a sueros hiperinmunes experimentales anti-suero de Caiman yacare y anti-suero de Caiman latirostris. Los sueros que se probaron fueron los homólogos de Caiman yacare, Caiman latirostris y los heterólogos de Alligator missisipiensis, Tupinambis merinae, Tupinambis rufescens, Chelonoidis chilensis, Clelia rustica, Waglerophis merremii, Lystrophys dorbignyi, Phyton molurus, Boa constrictor occidentalis, Eunectes notaeus, Crotalus durissus terrificus, Bothrops alternatus, Bothrops diporus, Bothrops jararaca, Bothrops jararacussu, Bothrops moojeni, Pitangus sulphuratus y Gallus gallus. La reactividad inmunoquímica se determinó mediante las técnicas de doble inmunodifusión y ELISA, mostrándose importante entre los sueros de los crocodrílidos y baja entre estos y los de las otras especies de reptiles estudiadas. Se observó mayor reactividad entre los antisueros anti-Caiman respecto a los sueros de Caiman latirostris y Caiman yacare que frente al suero de Alligator missisipiensis. Además, se encontró una fuerte reactividad entre ambos sueros anti-Caiman y el de Gallus gallus poniendo en evidencia la fuerte reactividad entre los sueros de arcosaurios. In order to study the immunochemical reactivity among sera from different species of reptiles regarding sera from Caiman, the immunoreactivity of sera from reptiles against antisera to Caiman yacare or anti-Caiman latirostris sera was studied. These hiperimmune sera were tested against sera from Alligator missisipiensis, Tupinambis merinae, Tupinambis rufescens, Chelonoidis chilensis, Clelia rustica, Waglerophis merremii, Lystrophys dorbignyi, Phyton molurus, Boa constrictor occidentalis, Eunectes notaeus, Crotalus durissus terrificus, Bothrops alternatus, Bothrops neuwiedii, Bothrops jararaca, Bothrops jararacussu, Bothrops moojeni, Pitangus sulphuratus and Gallus gallus. The immunochemical

  13. The complementarity-determining region sequences in IgY antivenom hypervariable regions

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    David Gitirana da Rocha

    2017-08-01

    Full Text Available The data presented in this article are related to the research article entitled "Development of IgY antibodies against anti-snake toxins endowed with highly lethal neutralizing activity" (da Rocha et al., 2017 [1]. Complementarity-determining region (CDR sequences are variable antibody (Ab sequences that respond with specificity, duration and strength to identify and bind to antigen (Ag epitopes. B lymphocytes isolated from hens immunized with Bitis arietans (Ba and anti-Crotalus durissus terrificus (Cdt venoms and expressing high specificity, affinity and toxicity neutralizing antibody titers were used as DNA sources. The VLF1, CDR1, CDR2, VLR1 and CDR3 sequences were validated by BLASTp, and values corresponding to IgY VL and VH anti-Ba or anti-Cdt venoms were identified, registered [Gallus gallus IgY Fv Light chain (GU815099/Gallus gallus IgY Fv Heavy chain (GU815098] and used for molecular modeling of IgY scFv anti-Ba. The resulting CDR1, CDR2 and CDR3 sequences were combined to construct the three - dimensional structure of the Ab paratope.

  14. Protease activated receptors (PARS) mediation in gyroxin biological activity; Mediacao dos receptores ativados por proteases (PARs) em atividades biologicas da giroxina

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    Silva, Jose Alberto Alves da

    2009-07-01

    Gyroxin is a serine protease enzyme from the South American rattlesnake (Crotalus durissus terrificus) venom; it is only partially characterized and has multiple activities. Gyroxin induces blood coagulation, blood pressure decrease and a neurotoxic behavior named barrel rotation. The mechanisms involved in this neurotoxic activity are not known. Whereas gyroxin is a member of enzymes with high potential to become a new drug with clinical applications such as thrombin, batroxobin, ancrod, tripsyn and kalicrein, it is important to find out how gyroxin works. The analysis on agarose gel electrophoresis and circular dichroism confirmed the molecules' integrity and purity. The gyroxin intravenous administration in mice proved its neurotoxicity (barrel rotation). In vivo studies employing intravital microscopy proved that gyroxin induces vasodilation with the participation of protease activated receptors (PARs), nitric oxide and Na+K+ATPase. The leukocytes' adherence and rolling counting indicated that gyroxin has no pro inflammatory activity. Gyroxin induced platelet aggregation, which was blocked by inhibitors of PAR1 and PAR4 receptors (SCH 79797 and tcY-NH{sub 2}, respectively). Finally, it was proved that the gyroxin temporarily alter the permeability of the blood brain barrier (BBB). Our study has shown that both the protease-activated receptors and nitric oxide are mediators involved in the biological activities of gyroxin. (author)

  15. Amino acid sequence and disulfide bond assignment of myotoxin a isolated from the venom of prairie rattlesnake (Crotalus viridis viridis)

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    Fox, J.W.; Elzinga, M.; Tu, A.T.

    1979-02-20

    The primary structure of myotoxin a, a myotoxin protein from the venom of the North American rattlesnake Crotalus viridis viridis, was determined and the position of the disulfide bonds assigned. The toxin was isolated, carboxymethylated, and cleaved by cyanogen bromide, and the resultant peptides were isolated. The cyanogen bromide peptides were subjected to amino acid sequence analysis. In order to assign the positions of the three disulfide bonds, the native toxin was cleaved sequentially with cyanogen bromide and trypsin. A two peptide unit connected by one disulfide bond was isolated and characterized, and a three-peptide unit connected by two disulfide bonds was isolated. One peptide in the three-peptide unit was identified as Cys-Cys-Lys. In order to establish the linkages between the peptides and Cys-Cys-Lys, one cycle of Edman degradation was carried out such that the Cys-Cys bond was cleaved. Upon isolation and analysis of the cleavage products, the disulfide bonds connecting the three peptides were determined. The positions of the disulfide bridges of myotoxin a were determined to be totally different from those of neurotoxins isolated from snake venoms. The sequence of myotoxin a was compared with the sequences of other snake venom toxins using the computer program RELATE to determine whether myotoxin a is similar to any other types of toxins. From the computer analysis, myotoxin a did not show any close relationship to other toxins except crotamine from the South American rattlesnake Crotalus durissus terrificus.

  16. Structural aspects of crotoxin modified by ionizing radiation; Aspectos estruturais da crotoxina modificada pela radiacao ionizante

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina Corleto de; Albero, Felipe Guimaraes; Zezell, Denise Maria; Spencer, Patrick Jack; Nascimento, Nanci do, E-mail: kcorleto@usp.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2010-03-15

    Ionizing radiation has proven to be an excellent tool for reducing the toxicity of venoms and isolated toxins, resulting in better immunogens for serum production, the only effective treatment in case of snake bites, and contributing to the welfare of serum-producing animals. Since the action of gamma radiation of venoms and toxins has not been yet fully clarified from the structural point of view, we proposed in this paper, to characterize the crotoxin, a venom protein of the species Crotalus durissus terrificus by Circular Dichroism (CD) and Infrared Spectroscopy (FTIR) techniques. After chromatographic techniques, crotoxin was irradiated with 2.0 kGy ({sup 60}Co source). The CD spectra obtained of native and irradiated crotoxin solutions showed changes between the samples in characteristic regions of -sheet and-helix . The Infrared analyse showed expressive changes in the spectra of the native and irradiated crotoxin (amide I band region). These tests showed that crotoxin when subjected to gamma radiation, showed changes in their structural conformation compared with the samples in the native state. Such changes probably occur in the secondary structure and may explain its neurotoxic activity loss. (author)

  17. Walker 256 Tumor Growth Suppression by Crotoxin Involves Formyl Peptide Receptors and Lipoxin A4

    Science.gov (United States)

    Brigatte, Patrícia; Faiad, Odair Jorge; Ferreira Nocelli, Roberta Cornélio; Landgraf, Richardt G.; Palma, Mario Sergio; Cury, Yara; Curi, Rui; Sampaio, Sandra Coccuzzo

    2016-01-01

    We investigated the effects of Crotoxin (CTX), the main toxin of South American rattlesnake (Crotalus durissus terrificus) venom, on Walker 256 tumor growth, the pain symptoms associated (hyperalgesia and allodynia), and participation of endogenous lipoxin A4. Treatment with CTX (s.c.), daily, for 5 days reduced tumor growth at the 5th day after injection of Walker 256 carcinoma cells into the plantar surface of adult rat hind paw. This observation was associated with inhibition of new blood vessel formation and decrease in blood vessel diameter. The treatment with CTX raised plasma concentrations of lipoxin A4 and its natural analogue 15-epi-LXA4, an effect mediated by formyl peptide receptors (FPRs). In fact, the treatment with Boc-2, an inhibitor of FPRs, abolished the increase in plasma levels of these mediators triggered by CTX. The blockage of these receptors also abolished the inhibitory action of CTX on tumor growth and blood vessel formation and the decrease in blood vessel diameter. Together, the results herein presented demonstrate that CTX increases plasma concentrations of lipoxin A4 and 15-epi-LXA4, which might inhibit both tumor growth and formation of new vessels via FPRs. PMID:27190493

  18. Structural aspects of crotalic venom proteins modified by ionizing radiation; Aspectos estruturais de proteinas do veneno crotalico modificadas por radiacao ionizante

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina Corleto de

    2010-07-01

    Snake bites are a serious public health problem, especially in subtropical countries. In Brazil, the Ministry of Health notified around 26 000 accidents in 2008. The genus Crotalus (rattlesnakes) accounts for approximately 7% of the total, with a high mortality rate of 72% when untreated with the specific serum, the only effective treatment in case of snake bites. In Brazil, the serum is produced in horses which, despite the large size, have a reduced lifespan due to the high toxicity of the antigen. Ionizing radiation has proven to be an excellent tool for reducing the toxicity of venoms and isolated toxins, resulting in better immunogens for serum production, and contributing to the welfare of serum producing animals. Since the action of gamma radiation on venoms and toxins has not been yet fully clarified from the structural point of view, we proposed in this paper, to characterize two toxins of the species Crotalus durissus terrificus: crotoxin and crotamine. After isolation of the toxins of interest by chromatographic techniques, they were subjected to structural analysis with the application of the following methods: Fluorescence, Circular Dichroism, Differential Calorimetry and Infrared Spectroscopy. These tests showed that both crotamine as crotoxin when subjected to gamma radiation, showed changes in their structural conformation compared with the samples in the native state. Such changes probably occur in the secondary and tertiary structure and may explain the changes on the biological activity of these toxins. (author)

  19. Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

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    A Della Torre

    2011-01-01

    Full Text Available Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins. The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9 and without (-S9 metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9 and showed signs of mutagenic activity in TA97a and TA102 (both -S9 strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties.

  20. Morphologic and morphometric analysis of Hepatozoon spp. (Apicomplexa, Hepatozoidae of snakes

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    Moço Tatiana Cristina

    2002-01-01

    Full Text Available Hepatozoon species are the most abundant hemoparasites of snakes. Its identification has been based mainly on the morphologic characterization of the gamonts in the peripheral blood of the vertebrate host and also of the cysts found in the internal organs of the vertebrate and invertebrate hosts. Using a computerized image analysis system, we studied five species of Hepatozoon from recently captured snakes in Botucatu, State of São Paulo, Brazil, to evaluate the importance of the morphology and morphometry of the gamonts for the characterization of Hepatozoon species and to analyze the morphologic changes induced in the erythrocytes by the parasite. The studied species were H. terzii of Boa constrictor amarali, Hepatozoon sp. of Crotalus durissusterrificus, H. philodryasi of Philodryas patagoniensis, and H. migonei and H. cyclagrasi of Hydrodynastes gigas. We observed three different groups, one of them including the species H. terzii, H. philodryasi and Hepatozoon sp. of C. durissus terrificus; and the other two consisting of H. migonei and H. cyclagrasi. Degree of alterations in the erythrocytes was variable and it may be useful for characterization of Hepatozoon species.

  1. Physico-chemical characterization of gamma rays irradiated crotamine

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina Corleto de; Spencer, Patrick Jack; Nascimento, Nanci do [Instituto de Pesquisas Energeticas e Nucleares (IPEN-CNEN/SP), Sao Paulo, SP (Brazil)], e-mail: kcorleto@usp.br

    2009-07-01

    Ionizing radiation can change the molecular structure and affect the biological properties of biomolecules. It has been employed to attenuate animal toxins. Crotamine, a toxin from Crotalus durissus terrificus (Cdt), is a highly basic polypeptide (pI - 10.3), with myotoxic activity and molecular weight of 4882 Da. It is composed of 42 amino acids residues and reticulated by three disulfide bonds. This study aimed the characterization of irradiated crotamine using Circular Dichroism (CD), Fluorescence Spectroscopy and Differential Scanning Calorimetry (DSC) techniques. We used size exclusion and ion-exchange chromatography to purify it from Cdt crude venom. The pure crotamine was irradiated with 2.0 kGy from a {sup 60}Co source. Native and irradiated crotamine were analyzed in a fluorescence spectrophotometer (Hitachi F-4500), under excitation wavelength at 275 nm and the emission was scanned from 300 to 500 nm. The analysis of fluorescence quenching showed that the irradiated form displayed a lower quantum yield when compared to the native form. CD spectra, obtained from a Jasco, J-180 spectropolarimeter, of native and irradiated crotamine solutions, showed a discrete change between the samples, from apparently ordered conformation to a random coil. Finally, the thermodynamics analysis, realized in a calorimeter METTLER TOLEDO, DSC 822e, showed that irradiation promoted changes in the calorimetric profile. Our results indicate that irradiation leads to progressive changes in the structure of the toxin, which could explain the decrease in myotoxic activity. (author)

  2. Physical and functional association of the Src family kinases Fyn and Lyn with the collagen receptor glycoprotein VI-Fc receptor gamma chain complex on human platelets.

    Science.gov (United States)

    Ezumi, Y; Shindoh, K; Tsuji, M; Takayama, H

    1998-07-20

    We have previously shown that uncharacterized glycoprotein VI (GPVI), which is constitutively associated and coexpressed with Fc receptor gamma chain (FcRgamma) in human platelets, is essential for collagen-stimulated tyrosine phosphorylation of FcRgamma, Syk, and phospholipase Cgamma2 (PLCgamma2), leading to platelet activation. Here we investigated involvement of the Src family in the proximal signals through the GPVI-FcRgamma complex, using the snake venom convulxin from Crotalus durissus terrificus, which specifically recognizes GPVI and activates platelets through cross-linking GPVI. Convulxin-coupled beads precipitated the GPVI-FcRgamma complex from platelet lysates. Collagen and convulxin induced tyrosine phosphorylation of FcRgamma, Syk, and PLCgamma2 and recruited tyrosine-phosphorylated Syk to the GPVI-FcRgamma complex. Using coprecipitation methods with convulxin-coupled beads and antibodies against FcRgamma and the Src family, we showed that Fyn and Lyn, but not Yes, Src, Fgr, Hck, and Lck, were physically associated with the GPVI-FcRgamma complex irrespective of stimulation. Furthermore, Fyn was rapidly activated by collagen or cross-linking GPVI. The Src family-specific inhibitor PP1 dose-dependently inhibited collagen- or convulxin-induced tyrosine phosphorylation of proteins including FcRgamma, Syk, and PLCgamma2, accompanied by a loss of aggregation and ATP release reaction. These results indicate that the Src family plays a critical role in platelet activation via the collagen receptor GPVI-FcRgamma complex.

  3. Physical and Functional Association of the Src Family Kinases Fyn and Lyn with the Collagen Receptor Glycoprotein VI-Fc Receptor γ Chain Complex on Human Platelets

    Science.gov (United States)

    Ezumi, Yasuharu; Shindoh, Keisuke; Tsuji, Masaaki; Takayama, Hiroshi

    1998-01-01

    We have previously shown that uncharacterized glycoprotein VI (GPVI), which is constitutively associated and coexpressed with Fc receptor γ chain (FcRγ) in human platelets, is essential for collagen-stimulated tyrosine phosphorylation of FcRγ, Syk, and phospholipase Cγ2 (PLCγ2), leading to platelet activation. Here we investigated involvement of the Src family in the proximal signals through the GPVI–FcRγ complex, using the snake venom convulxin from Crotalus durissus terrificus, which specifically recognizes GPVI and activates platelets through cross-linking GPVI. Convulxin-coupled beads precipitated the GPVI–FcRγ complex from platelet lysates. Collagen and convulxin induced tyrosine phosphorylation of FcRγ, Syk, and PLCγ2 and recruited tyrosine-phosphorylated Syk to the GPVI–FcRγ complex. Using coprecipitation methods with convulxin-coupled beads and antibodies against FcRγ and the Src family, we showed that Fyn and Lyn, but not Yes, Src, Fgr, Hck, and Lck, were physically associated with the GPVI–FcRγ complex irrespective of stimulation. Furthermore, Fyn was rapidly activated by collagen or cross-linking GPVI. The Src family–specific inhibitor PP1 dose-dependently inhibited collagen- or convulxin-induced tyrosine phosphorylation of proteins including FcRγ, Syk, and PLCγ2, accompanied by a loss of aggregation and ATP release reaction. These results indicate that the Src family plays a critical role in platelet activation via the collagen receptor GPVI–FcRγ complex. PMID:9670039

  4. Biodistribution and SPECT imaging of {sup 125/131}I-crotoxin on mice bearing Ehrlich solid tumour

    Energy Technology Data Exchange (ETDEWEB)

    Soares, Marcella Araugio; Santos, Raquel Gouvea dos [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)], e-mail: marcellaaraugio@yahoo.com.br, e-mail: santosr@cdtn.br; Silveira, Marina B.; Simal, Carlos [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Faculdade de Medicina; Dias, Consuelo L. Fortes [Fundacao Ezequiel Dias (FUNED), Belo Horizonte, MG (Brazil)

    2009-07-01

    The search of specific radiopharmaceuticals to be used in breast tumour diagnosis is relevant to complement the techniques applied in conventional medicine. Crotalus durissus terrificus venom (CV) and its main polypeptide, Crotoxin (Crtx), are natural source of several bioactive substances with therapeutical potential. The aim of this work was to evaluate the binding of Crtx with tumour targets in vivo, as well as, evaluate its applicability for breast tumours diagnosis. Crtx was labelled with {sup 125/131}I using lactoperoxidase method and radiochemical analysis was performed by chromatography. {sup 125}I-Crtx was used for biodistribution and pharmacokinetics studies on swiss mice bearing Ehrlich solid tumour, while {sup 131}I-Crtx was used for single photon emission computed tomography (SPECT) imaging. Crtx presented specific binding sites on Ehrlich tumour cells and had a rapid blood clearance (T{sub 1/2}= 201.1 min.). Intratumoral administration increased significantly the activity delivered into the tumour site (128-fold higher) and reduced the kidney burden (7.2-fold lower). {sup 131}I-Crxt demonstrated to interact with tumour cells for until 72 hours allowing good quality images of tumour. Our results indicate the biotechnological potential of Crtx as template for radiopharmaceutical design for cancer diagnosis. (author)

  5. Novo método de dosagem de soros antipeçonhentos em camundongos lactentes: I - dosagem do soro anticrotálico New method for dosage of antivenoms sera in baby mice: I-Anticrotalic serum's dosage

    Directory of Open Access Journals (Sweden)

    Bruno Soerensen

    1984-08-01

    Full Text Available A atividade biológica dos soros antipeçonhentos pode ser determinada "in vivo" em pombos, coelhos, cobaios e camundongos adultos, não existindo, entretanto, nenhum método que possa ser recomendado internacionalmente. Estudou-se comparativamente aos métodos tradicionais de dosagem de soros em pombos e camundongos adultos, a validade do uso de camundongos lactentes de 6 a 7 dias, pesando 4 a 5g, inoculados pela via subcutânea. Inicialmente foi determinada a toxidez do veneno de Crotalus durissus terríficus através do estudo da sintomatologia do envenenamento e da atividade letal. O estudo comparativo dos três métodos forneceu maior concordância de resultados em DE100 entre as dosagens realizadas com camundongos lactentes e adultos. A DE100 e DL50 determinadas em camundongos lactentes forneceu resultados mais constantes que os dos métodos de camundongos adultos e pombos. O uso deste novo método permite eliminar a dificuldade de obtenção do atual animal de prova; a utilização de um maior número de animais por ponto de avaliação biológica, possibilitando uma maior precisão e conseguindo-se uma uniformidade nas características exigidas neste tipo de dosagem como peso, idade e linhagem, visando a reprodução sistemática dos resultados.The biological activity of antiserum against venoms can be assessed by "in vivo" tests using pigeons, rabbits, guinea-pigs and adult mice although there is no International standardized reaction for it. The traditional methods using pigeons and adult mice were comparatively studied with those using mice of 4-5 g (6-7 days of age, inoculated by subcutaneous injection. The toxicity of the Crotalus durissus terrificus venom was initially determined by studying the symptomatology after administration of venom and lethal activity. The comparative study of these three methods showed that the best concordance in the results of the ED100 was obtained using adult and baby mice. The most homogeneous ED100

  6. Alterações hematológicas em bovinos submetidos ao envenenamento crotálico

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    P. M. Ferreira

    2005-03-01

    Full Text Available RESUMO: O quadro clínico observado ao exame do animal acidentado pela cascavél sul americana (Crotalus durissus terrificus , portadora de veneno contendo quantidades significativas da toxina crotamina, é de difícil diferenciação clínica com outras doenças. Com o objetivo de se estabelecer alterações hematológicas que auxiliem o diagnóstico desta condição mórbida, cinco fêmeas bovinas mestiças, com idade variando entre dois e três anos, foram inoculadas com veneno crotálico do tipo crotamina positivo, utilizando a dose de 0,03 mg por quilo de pêso vivo, na região glútea, por via intramuscular. Colheu-se amostras de sangue para realizar os exames laboratoriais antes e a cada duas horas após a inoculação, até a morte dos animais. Verificou-se que: o número de hemácias, o volume corpuscular médio ( VCM , a hemoglobina corpuscular média ( HCM , os números de leucócitos totais, de linfócitos, de neutrófilos e de monócitos, tiveram aumentos significativos e que os números de leucócitos totais apresentaram comportamento atípico, caracterizado por leucocitoses com valores atingindo até 60.000 celulas/mm,3 a cada seis horas, alternadas por quedas para em torno de 13.000 células/mm3. Pelo exposto, concluiu-se que a observação destas alterações poderá auxiliar o estabelecimento do diagnóstico desta afecção nos bovinos. PALAVRAS CHAVE: Envenenamento crotálico; hemograma; bovinos. SUMMARY: The clinical findings observed at examination of an animal attacked by South American rattle snake (Crotalus durissus terrificus , that has poison containing significative crotamine toxin quantites are difficult to distinguish from other diseases. With the aim of establishing haematological parameters of diagnostic value for this condition diagnosis, five crossbred female cattle, from two to three years old, were inoculated with

  7. In Vitro Antiophidian Mechanisms of Hypericum brasiliense Choisy Standardized Extract: Quercetin-Dependent Neuroprotection

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    Cháriston André Dal Belo

    2013-01-01

    Full Text Available The neuroprotection induced by Hypericum brasiliense Choisy extract (HBE and its main active polyphenol compound quercetin, against Crotalus durissus terrificus (Cdt venom and crotoxin and crotamine, was enquired at both central and peripheral mammal nervous system. Cdt venom (10 μg/mL or crotoxin (1 μg/mL incubated at mouse phrenic nerve-diaphragm preparation (PND induced an irreversible and complete neuromuscular blockade, respectively. Crotamine (1 μg/mL only induced an increase of muscle strength at PND preparations. At mouse brain slices, Cdt venom (1, 5, and 10 μg/mL decreased cell viability. HBE (100 μg/mL inhibited significantly the facilitatory action of crotamine (1 μg/mL and was partially active against the neuromuscular blockade of crotoxin (1 μg/mL (data not shown. Quercetin (10 μg/mL mimicked the neuromuscular protection of HBE (100 μg/mL, by inhibiting almost completely the neurotoxic effect induced by crotoxin (1 μg/mL and crotamine (1 μg/mL. HBE (100 μg/mL and quercetin (10 μg/mL also increased cell viability in mice brain slices. Quercetin (10 μg/mL was more effective than HBE (100 μg/mL in counteracting the cell lysis induced by Cdt venom (1 and 10 μg/mL, resp.. These results and a further phytochemical and toxicological investigations could open new perspectives towards therapeutic use of Hypericum brasiliense standardized extract and quercetin, especially to counteract the neurotoxic effect induced by snake neurotoxic venoms.

  8. Ação neuro-muscular do veneno crotálico: dados preliminares Neuromuscular action of crotalid venom: preliminar data

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    Maria Dorvalina Silva

    1996-03-01

    Full Text Available Estudamos 6 pacientes, 2 cães e um coelho com intoxicação crotálica. Avaliamos a condução nervosa periférica sensitiva e motora, a transmissão neuromuscular e eletromiografias. As biópsias de músculo foram processadas por histoquímica. Os 6 pacientes apresentaram mononeuropatia sensitiva no nervo periférico adjacente ao local da inoculação do veneno e encontramos evidências histoquímicas de miopatia mitocondrial. Os defeitos da transmissão neuromuscular foram mínimos. A maioria dos autores admite que veneno crotálico determina síndrome miastênica. Nossos achados indicam que ptose palpebral, facies miastênico e fraqueza muscular observados após acidente crotálico, correspondem provavelmente a miopatia mitocondrial, muitas vezes transitória e reversível.We studied 6 patients and 2 dogs that have been bitten by South American rattlesnake Crotalus durissus terrificus and one rabbit inoculated with crotalid venom. We analized sensory and motor peripheral nerve conduction, repetitive stimulation for studying neuromuscular transmission and electromyographies. Muscle biopsies were processed by histochemistry. All patients had peripheral mononeuropathy of the closest sensitive nerve to the area of snakebite. The neuromuscular transmission alterations were minimal. Muscle histochemistry of 4 patients, 2 dogs and 1 rabbit showed findings of mitochondrial myopathy. The majority of authors admit that crotalid venom causes myastenic syndrome. Our findings suggest that palpebral ptosis, myastenic facies and muscular weakness observed after crotalid poisoning are, probably, due to transient and reversible mitochondrial myopathy. As far as we know, this is the first report on the ability of the venom of this rattlesnake to cause local sensitive mononeuropathy and the first muscle histochemistry showing mitochondrial myopathy in humans poisoned by crotalid venom.

  9. Crotoxin in humans: analysis of the effects on extraocular and facial muscles

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    Geraldo de Barros Ribeiro

    2012-12-01

    Full Text Available PURPOSE: Crotoxin is the main neurotoxin of South American rattlesnake Crotalus durissus terrificus. The neurotoxic action is characterized by a presynaptic blockade. The purpose of this research is to assess the ability of crotoxin to induce temporary paralysis of extraocular and facial muscles in humans. METHODS: Doses of crotoxin used ranged from 2 to 5 units (U, each unit corresponding to one LD50. We first applied 2U of crotoxin in one of the extraocular muscles of 3 amaurotic individuals to be submitted to ocular evisceration. In the second stage, we applied crotoxin in 12 extraocular muscles of 9 patients with strabismic amblyopia. In the last stage, crotoxin was used in the treatment of blepharospasm in another 3 patients. RESULTS: No patient showed any systemic side effect or change in vision or any eye structure problem after the procedure. The only local side effects observed were slight conjunctival hyperemia, which recovered spontaneously. In 2 patients there was no change in ocular deviation after 2U crotoxin application. Limitation of the muscle action was observed in 8 of the 12 applications. The change in ocular deviation after application of 2U of crotoxin (9 injections was in average 15.7 prism diopters (PD. When the dose was 4U (2 applications the change was in average 37.5 PD and a single application of 5U produced a change of 16 PD in ocular deviation. This effect lasted from 1 to 3 months. Two of the 3 patients with blepharospasm had the hemifacial spasm improved with crotoxin, which returned after 2 months. CONCLUSIONS: This study provides data suggesting that crotoxin may be a useful new therapeutic option for the treatment of strabismus and blepharospasm. We expect that with further studies crotoxin could be an option for many other medical areas.

  10. Anti-tumor activity of CrTX in human lung adenocarcinoma cell line A549

    Institute of Scientific and Technical Information of China (English)

    Bin YE; Yan XIE; Zheng-hong QIN; Jun-chao WU; Rong HAN; Jing-kang HE

    2011-01-01

    Aim:To assess the cytotoxic effect of crotoxin (CrTX),a potent neurotoxin extracted from the venom of the pit viper Crotalus durissus terrificus,in human lung adenocarcinoma A549 cells and investigated the underlying mechanisms.Methods:A549 cells were treated with gradient concentrations of CrTX,and the cell cycle and apoptosis were analyzed using a flow cytometric assay.The changes of cellular effectors p53,caspase-3 and cleaved caspase-3,total P38MAPK and pP38MAPK were investigated using Western blot assays.A549 xenograft model was used to examine the inhibition of CrTX on tumor growth in vivo.Results:Treatment of A549 cells with CrTX (25-200 μg/mL) for 48 h significantly inhibited the cell growth in a dose-dependent manner (IC50=78 μg/mL).Treatment with CrTX (25 iJg/mL) for 24 h caused G1 arrest and induced cell apoptosis.CrTX (25 μg/mL) significantly increased the expression of wt p53,cleaved caspase-3 and phospho-P38MAPK.Pretreatment with the specific P38MAPK inhibitor SB203580 (5 μmol/L) significantly reduced CrTX-induced apoptosis and cleaved caspase-3 level,but G1 arrest remained unchanged and highly expressed p53 sustained.Intraperitoneal injection of CrTX (10 μg/kg,twice a week for 4 weeks) significantly inhibited A549 tumor xenograft growth,and decreased MVD and VEGF levels.Conclusion:CrTX produced significant anti-tumor effects by inducing cell apoptosis probably due to activation of P38MAPK and caspase-3,and by cell cycle arrest mediated by increased wt p53 expression.In addition,CrTX displayed anti-angiogenic effects in vivo.

  11. Autophagy is involved in cytotoxic effects of crotoxin in human breast cancer cell line MCF-7 cells

    Institute of Scientific and Technical Information of China (English)

    Ci-hui YAN; Ya-ping YANG; Zheng-hong QIN; Zhen-lun GU; Paul REID; Zhong-qin LIANG

    2007-01-01

    Aim: To investigate the role of crotoxin (CrTX)-induced autophagy in the death of MCF-7 cells, a caspase-3-deficient, human breast cancer cell line. Methods: Cul-tured MCF-7 cells were treated with various doses of CrTX, a phospholipase A2(PLA2) isolated from the venom of the South American rattlesnake, Crotalus durissus terrificus. The cytotoxicity of CrTX in the presence and absence of caspase inhibitors was measured with methyl thiazolyl tetrazolium (MTT) and lactate dehydrogenase (LDH) leakage assays. The activation of autophagy was determined with transmission electron microscope and monodansylcadaverin(MDC) labeling. The upregulation of lysosomal enzymes, the release of cyto-chrome c (cyto-c), and the nuclear translocation of the apoptosis inducing factor(AIF) were examined by immunoblotting and immunofluorescence. Results: CrTX inhibited the viability of MCF-7 cells in a dose- and time-dependent manner. CrTX-activated autophagy was revealed by punctuate MDC labeling, and an increase in the formation of autophagosomes as well as apoptosis, as evidenced by nuclear condensation and fragmentation. The activation of cathepsin B, D, and L, in addition to the release of cytochrome c and the relocation of AIF into nuclei, were observed after CrTX treatment. Autophagy inhibitors 3-methyladenine (3-MA),NH4Cl, and the pan-caspase inhibitor, Z-Val-Ala-Asp-fluoromethylketone (Z-Vad-fmk), attenuated CrTX-induced cell death. Conclusion: An autophagic mecha-nism contributes to the apoptosis of MCF-7 cells induced by CrTX.

  12. Structural analysis of irradiated crotoxin by spectroscopic techniques

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina C. de; Fucase, Tamara M.; Silva, Ed Carlos S. e; Chagas, Bruno B.; Buchi, Alisson T.; Viala, Vincent L.; Spencer, Patrick J.; Nascimento, Nanci do, E-mail: kcorleto@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Biotecnologia

    2013-07-01

    Snake bites are a serious public health problem, especially in subtropical countries. In Brazil, the serum, the only effective treatment in case of snake bites, is produced in horses which, despite of their large size, have a reduced lifespan due to the high toxicity of the antigen. Ionizing radiation has been successfully employed to attenuate the biological activity of animal toxins. Crotoxin, the main toxic compound from Crotalus durissus terrificus (Cdt), is a heterodimeric protein composed of two subunits: crotapotin and phospholipase A{sub 2}. Previous data indicated that this protein, following irradiation process, undergoes unfolding and/or aggregation, resulting in a much lower toxic antigen. The exact mechanisms and structural modifications involved in aggregation process are not clear yet. This work investigates the effects of ionizing radiation on crotoxin employing Infrared Spectroscopy, Circular Dichroism and Dynamic Light Scattering techniques. The infrared spectrum of lyophilized crotoxin showed peaks corresponding to the vibrational spectra of the secondary structure of crotoxin, including β-sheet, random coil, α-helix and β-turns. We calculated the area of these spectral regions after adjusting for baseline and normalization using the amide I band (1590-1700 cm{sup -1}), obtaining the variation of secondary structures of the toxin following irradiation. The Circular Dichroism spectra of native and irradiated crotoxin suggests a conformational change within the molecule after the irradiation process. This data indicates structural changes between the samples, apparently from ordered conformation towards a random coil. The analyses by light scattering indicated that the irradiated crotoxin formed multimers with an average molecular radius 100 folds higher than the native toxin. (author)

  13. Binding studies of the antitumoral radiopharmaceutical 125I-Crotoxin to Ehrlich ascites tumor cells

    Energy Technology Data Exchange (ETDEWEB)

    Silveira, Marina B.; Santos, Raquel G. dos [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Dias, Consuelo L. Fortes [Fundacao Ezequiel Dias (FUNED), Belo Horizonte, MG (Brazil)], e-mail: consuelo@pq.cnpq.br; Cassali, Geovanni D. [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Lab. de Patologia Comparada], e-mail: cassalig@icb.ufmg.br

    2009-07-01

    The development of tools for functional diagnostic imaging is mainly based on radiopharmaceuticals that specifically target membrane receptors. Crotoxin (Crtx), a polypeptide isolated from Crotalus durissus terrificus venom, has been shown to have an antitumoral activity and is a promising bioactive tracer for tumor detection. More specific radiopharmaceuticals are being studied to complement the techniques applied in the conventional medicine against breast cancer, the most frequent cause of death from malignant disease in women. Crtx's effect has been shown to be related with the overexpression of epidermal growth factor receptor (EGFR), present in high levels in 30 to 60% of breast tumor cells. Our objective was to evaluate Crtx as a tracer for cancer diagnosis, investigating its properties as an EGFR-targeting agent. Ehrlich ascites tumor cells (EAT cells) were used due to its origin and similar characteristics to breast tumor cells, specially the presence of EGFR. Crtx was labeled with 125I and binding experiments were performed. To evaluate the specific binding in vitro of Crtx, competition binding assay was carried out in the presence of increasing concentrations of non-labelled crotoxin and epidermal growth factor (EGF). Specific binding of 125I-Crtx to EAT cells was determined and the binding was considered saturable, with approximately 70% of specificity, high affinity (Kd = 19.7 nM) and IC50 = 1.6 x 10-11 M. Our results indicate that Crtx's interaction with EAT cells is partially related with EGFR and increases the biotechnological potential of Crtx as a template for radiopharmaceutical design for cancer diagnosis. (author)

  14. Microbiological evaluation of different strategies for management of snakes in captivity.

    Science.gov (United States)

    Campagner, M V; Bosco, S M G; Bagagli, E; Cunha, M L R S; Jeronimo, B C; Saad, E; Biscola, N P; Ferreira, R S; Barraviera, B

    2012-01-01

    Keeping snakes in captivity to produce venom for scientific research and production of inputs is now a worldwide practice. Maintaining snakes in captivity involves capture, infrastructure investments, management techniques, and appropriate qualified personnel. Further, the success of the project requires knowledge of habitat, nutrition, and reproduction, and control of opportunistic infections. This study evaluated the management of snakes in three types of captivity (quarantine, intensive, and semiextensive) and diagnosed bacterial and fungal contaminants. A bacteriological profile was obtained by swabbing the oral and cloacal cavities, scales, and venoms of healthy adult snakes from Bothrops jararaca (Bj) and Crotalus durissus terrificus (Cdt). There was predominance of Enterobacteriaceae, especially non-fermenting Gram-negative bacilli excluding Pseudomonas spp and Gram- positive bacteria. Statistically, intensive captivity resulted in the highest number of bacterial isolates, followed by recent capture (quarantine) and by semiextensive captivity. No statistical difference was found between Bj and Cdt bacterial frequency. In vitro bacterial susceptibility testing found the highest resistance against the semisynthetic penicillins (amoxicillin and ampicillin) and highest sensitivity to amicacin and tobramycin aminoglycosides. To evaluate mycological profile of snakes from intensive captivity, samples were obtained from two healthy Bj and one B. moojeni, one B. pauloensis, and one Cdt showing whitish lesions on the scales suggestive of ringworm. Using conventional methods and DNA-based molecular procedures, five samples of Trichosporon asahii were identified. Despite the traditional role of intense captivity in ophidian venom production, semiextensive captivity was more effective in the present study by virtue of presenting superior control of bacterial and fungal transmission, easier management, lowest cost, and decreased rate of mortality; therefore, it should be

  15. Study of crotoxin on the induction of paralysis in extraocular muscle in animal model

    Directory of Open Access Journals (Sweden)

    Geraldo de Barros Ribeiro

    2012-10-01

    Full Text Available PURPOSE: Crotoxin is the major toxin of the venom of the South American rattlesnake Crotalus durissus terrificus, capable of causing a blockade of the neurotransmitters at the neuromuscular junction. The objective of this study was to appraise the action and effectiveness of the crotoxin induced paralysis of the extraocular muscle and to compare its effects with the botulinum toxin type A (BT-A. METHODS: The crotoxin, with LD50 of 1.5 µg, was injected into the superior rectus muscle in ten New Zealand rabbits. The concentration variance was 0.015 up to 150 µg. Two rabbits received 2 units of botulinum toxin type A for comparative analysis. The evaluation of the paralysis was performed using serial electromyography. After the functional recovery of the muscles, which occurred after two months, six rabbits were sacrificed for anatomopathology study. RESULTS: The animals did not show any evidence of systemic toxicity. Transitory ptosis was observed in almost every animal and remained up to fourteen days. These toxins caused immediate blockade of the electrical potentials. The recovery was gradual in the average of one month with regeneration signs evident on the electromyography. The paralysis effect of the crotoxin on the muscle was proportional to its concentration. The changes with 1.5 µg crotoxin were similar to those produced by the botulinum toxin type A. The histopathology findings were localized to the site of the injection. No signs of muscle fiber's necrosis were seen in any sample. The alterations induced by crotoxin were also proportional to the concentration and similar to botulinum toxin type A in concentration of 1.5 µg. CONCLUSION: Crotoxin was able to induce transitory paralysis of the superior rectus muscle. This effect was characterized by reduction of action potentials and non-specific signs of fibrillation. Crotoxin, in concentration of 1.5 µg was able to induce similar effects as botulinum toxin type A.

  16. Rattlesnake Phospholipase A2 Increases CFTR-Chloride Channel Current and Corrects ∆F508CFTR Dysfunction: Impact in Cystic Fibrosis.

    Science.gov (United States)

    Faure, Grazyna; Bakouh, Naziha; Lourdel, Stéphane; Odolczyk, Norbert; Premchandar, Aiswarya; Servel, Nathalie; Hatton, Aurélie; Ostrowski, Maciej K; Xu, Haijin; Saul, Frederick A; Moquereau, Christelle; Bitam, Sara; Pranke, Iwona; Planelles, Gabrielle; Teulon, Jacques; Herrmann, Harald; Roldan, Ariel; Zielenkiewicz, Piotr; Dadlez, Michal; Lukacs, Gergely L; Sermet-Gaudelus, Isabelle; Ollero, Mario; Corringer, Pierre-Jean; Edelman, Aleksander

    2016-07-17

    Deletion of Phe508 in the nucleotide binding domain (∆F508-NBD1) of the cystic fibrosis transmembrane regulator (CFTR; a cyclic AMP-regulated chloride channel) is the most frequent mutation associated with cystic fibrosis. This mutation affects the maturation and gating of CFTR protein. The search for new high-affinity ligands of CFTR acting as dual modulators (correctors/activators) presents a major challenge in the pharmacology of cystic fibrosis. Snake venoms are a rich source of natural multifunctional proteins, potential binders of ion channels. In this study, we identified the CB subunit of crotoxin from Crotalus durissus terrificus as a new ligand and allosteric modulator of CFTR. We showed that CB interacts with NBD1 of both wild type and ∆F508CFTR and increases their chloride channel currents. The potentiating effect of CB on CFTR activity was demonstrated using electrophysiological techniques in Xenopus laevis oocytes, in CFTR-HeLa cells, and ex vivo in mouse colon tissue. The correcting effect of CB was shown by functional rescue of CFTR activity after 24-h ΔF508CFTR treatments with CB. Moreover, the presence of fully glycosylated CFTR was observed. Molecular docking allowed us to propose a model of the complex involving of the ABCβ and F1-like ATP-binding subdomains of ΔF508-NBD1. Hydrogen-deuterium exchange analysis confirmed stabilization in these regions, also showing allosteric stabilization in two other distal regions. Surface plasmon resonance competition studies showed that CB disrupts the ∆F508CFTR-cytokeratin 8 complex, allowing for the escape of ∆F508CFTR from degradation. Therefore CB, as a dual modulator of ΔF508CFTR, constitutes a template for the development of new anti-CF agents.

  17. Biochemical and pharmacological characterization of irradiated crotamine by gamma rays of {sup 60}Co; Caracterizacao bioquimica e farmacologica da crotamina irradiada por raios gama de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina Corleto

    2014-07-01

    The serum production in Brazil, the only effective treatment in cases of snakebites, uses horses that although large size, have reduced l lifespan compared with horses not immunized. Ionizing radiation has been shown as an excellent tool in reducing the toxicity of venoms and toxins isolated, and promote the achievement of better immunogens for serum production, and contributing to the welfare of serum-producing animals. It is known, however, that the effects of ionizing radiation on protein are characterized by various chemical modifications, such as fragmentation, cross-linking due to aggregation and oxidation products generated by water radiolysis. However, the action of gamma radiation on toxins is not yet fully understood structurally and pharmacologically, a fact that prevents the application of this methodology in the serum production process. So we proposed in this paper the characterization of crotamine, an important protein from the venom of Crotalus durissus terrificus species, irradiated with {sup 60}Co gamma rays. After isolating the toxin by chromatographic techniques and testing to prove the obtaining of pure crotamine, it was irradiated with gamma rays and subjected to structural analysis, Fluorescence and Circular Dichroism. Using high hydrostatic pressure tests were also conducted in order to verify that the conformational changes caused by radiation suffer modifications under high pressures. From the pharmacological point of view, muscle contraction tests were conducted with the objective of limiting the action of crotamine in smooth muscle as well as the change in the action of toxin caused structural changes to the front. Analysis of Circular Dichroism and Fluorescence showed changes in structural conformation of crotamine when subjected to gamma radiation and that such changes possibly occurring in the secondary and tertiary structure of the protein. The observed in pharmacological tests showed that the irradiated crotamine was less effective

  18. Vipericidins: a novel family of cathelicidin-related peptides from the venom gland of South American pit vipers.

    Science.gov (United States)

    Falcao, C B; de La Torre, B G; Pérez-Peinado, C; Barron, A E; Andreu, D; Rádis-Baptista, G

    2014-11-01

    Cathelicidins are phylogenetically ancient, pleiotropic host defense peptides-also called antimicrobial peptides (AMPs)-expressed in numerous life forms for innate immunity. Since even the jawless hagfish expresses cathelicidins, these genetically encoded host defense peptides are at least 400 million years old. More recently, cathelicidins with varying antipathogenic activities and cytotoxicities were discovered in the venoms of poisonous snakes; for these creatures, cathelicidins may also serve as weapons against prey and predators, as well as for innate immunity. We report herein the expression of orthologous cathelicidin genes in the venoms of four different South American pit vipers (Bothrops atrox, Bothrops lutzi, Crotalus durissus terrificus, and Lachesis muta rhombeata)-distant relatives of Asian cobras and kraits, previously shown to express cathelicidins-and an elapid, Pseudonaja textilis. We identified six novel, genetically encoded peptides: four from pit vipers, collectively named vipericidins, and two from the elapid. These new venom-derived cathelicidins exhibited potent killing activity against a number of bacterial strains (S. pyogenes, A. baumannii, E. faecalis, S. aureus, E. coli, K. pneumoniae, and P. aeruginosa), mostly with relatively less potent hemolysis, indicating their possible usefulness as lead structures for the development of new anti-infective agents. It is worth noting that these South American snake venom peptides are comparable in cytotoxicity (e.g., hemolysis) to human cathelicidin LL-37, and much lower than other membrane-active peptides such as mastoparan 7 and melittin from bee venom. Overall, the excellent bactericidal profile of vipericidins suggests they are a promising template for the development of broad-spectrum peptide antibiotics.

  19. The unequal influences of the left and right vagi on the control of the heart and pulmonary artery in the rattlesnake, Crotalus durissus

    DEFF Research Database (Denmark)

    Taylor, E.W.; Andrade, Denis V.; Abe, Augusto A.

    2009-01-01

    Autonomic control of the cardiovascular system in reptiles includes sympathetic components but heart rate (fH), pulmonary blood flow ( pul) and cardiac shunt patterns are primarily controlled by the parasympathetic nervous system. The vagus innervates both the heart and a sphincter on the pulmonary...

  20. Anatomia descritiva e comparativa do sistema urinário de Crotalus durissus Linnaeus, 1758, Bothrops neuwiedi Wagler, 1824 e B. moojeni Hoge, 1965 (Ophidia, Viperidae)

    OpenAIRE

    Silva, Luisa Helena Rocha da

    2008-01-01

    As serpentes desenvolveram uma imensa variedade de especializações quanto ao uso do habitat e nicho ocupados ao longo de sua evolução. As diferenças mais notáveis estão na forma, no tamanho do corpo e na textura superficial, o que reflete na diversidade de comportamentos e adaptações ecológicas e fisiológicas. Apesar do número de espécies e por isso mesmo, das diversas possibilidades de estudos que poderiam ser realizados, poucos são os trabalhos abordando a anatomia e fisiologia das serpente...

  1. Aspectos clínico-patológicos e laboratoriais do envenenamento crotálico experimental em equinos

    Directory of Open Access Journals (Sweden)

    Cinthia Távora A. Lopes

    2012-09-01

    Full Text Available Descrevem-se os quadros clínico-patológicos e laboratoriais de equinos inoculados experimentalmente com a peçonha de Caudisona durissa terrificus (Crotalus durissus terrificus na antiga nomenclatura, com a finalidade de fornecer subsídios que favoreçam a compreensão desse tipo de acidente ofídico em equinos. O veneno liofilizado foi diluído em 1ml de solução salina a 0,9% e inoculado por via subcutânea em cinco equinos, nas doses de 0,12mg/kg (um animal, 0,066mg/kg (dois animais e 0,03mg/kg (dois animais. O veneno causou a morte do equino que recebeu a dose de 0,12mg/kg e de um dos dois que receberam a dose de 0,066mg/kg, com evolução de 27h27min e 52h29min, respectivamente. O segundo animal que recebeu a dose de 0,066mg/kg também adoeceu, mas recuperou-se após 12 dias da inoculação. A dose de 0,03mg/kg determinou quadros não fatais do envenenamento, com período de evolução que variou entre 6 e 10 dias. O quadro clínico caracterizou-se por considerável aumento de volume no local de inoculação (escápula que se estendeu por todo o membro, apatia e cabeça baixa, alterações locomotoras evidenciadas pelo arrastar das pinças no solo, decúbito e dificuldade para levantar, redução dos reflexos auricular, palatal, do lábio superior e de ameaça, e aumento das frequências cardíaca e respiratória. Os exames laboratoriais revelaram leucocitose por neutrofilia e linfocitose em apenas dois animais. Houve aumento das enzimas creatina quinase (CK, dehidrogenase láctica (DHL e da ureia, e também redução nos níveis séricos de cálcio, fósforo e magnésio. O tempo de tromboplastina parcial ativada (TTPA aumentou nos equinos que morreram. Os achados de necropsia foram edema do tecido subcutâneo em todo o membro em que foi aplicado o veneno, sufusões no epicárdio dos ventrículos cardíacos esquerdo e direito, e bexiga com áreas hemorrágicas em grande parte da mucosa. Ao exame histopatológico observaram-se f

  2. Use of I-131- CRTX for targeting malignant adenocarcinoma in mice: biodistribution and radiation dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Raquel Gouvea dos; Soares, Marcella Araugio; Andrade, Henrique Martins de, E-mail: santosr@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Santos, Marcos Antonio da Cunha [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Departamento de Estatistica

    2008-07-01

    Snake venoms molecules have been shown to play a role not only in the survival and proliferation of tumour cells but also in the processes of tumour cell adhesion, migration and angiogenesis. We have shown that {sup 125}I-Crtx, a radiolabeled version of a protein derived from Crotalus durissus terrificus snake venom (Cdt), specifically binds to tumor and triggers apoptotic death. This study reports the biokinetic profile of {sup 99m}Tc-Cdt and {sup 125/131}I-Crtx in Swiss mice bearing Ehrlich solid tumor and MIRD formulation was applied to calculate the absorbed radiation doses for various organs and tumor site. Biokinetic evaluations were performed up to 24 h after intravenous (i.v) or intratumor (i.tu.) injection of {sup 99m}Tc-Cdt or {sup 125/131}I-Crtx. Time-activity curves were generated for the main organs by fitting the organ specific mass mean counts. The radiation dose from {sup 131}I-Crtx was calculated based on non penetrating radiation in the mouse model. Biokinetics data from {sup 99m}Tc-Cdt after i.v. injection in mice tumor model showed rapid blood clearance (T{sub 1/2}= 36.1 ± 2.4 min.), slow tumor clearance (T{sub 1/2}: 108.3 ± 19.5 min.) and indicated the kidneys as the main excretion pathway. Interaction studies in vitro demonstrated that {sup 125}I-Crtx recognize specific sites on Erlich tumor cell membrane. Upon intravenous and intratumor administration of {sup 131}I-Crtx in mice bearing Erlich tumor, it was observed high uptake in tumor site in vivo (Ã =72kBq x h/g) resulting in a high absorbed dose radiation to tumor site. Distributions of {sup 125/131}I-Crtx i.v. were only significant in tumor, stomach, liver and kidneys, reflecting non-specific uptake of Crtx in normal excretion tissues in vivo. Intratumoral administration reduced significantly the radiation dose to the kidneys (42-fold lower) and increased the uptake by the tumor site (128- fold higher). {sup 131}I control was run in a parallel experiment and showed no significant tumor

  3. Obtainment of crotoxin/phema-g-LDPE and crotoxin/PCL systems;Obtencao dos sistemas bioconjugados crotoxina/PEBD-g-phema e crotoxina/PCL

    Energy Technology Data Exchange (ETDEWEB)

    Lorenzetti, Solange Gubbelini

    2006-07-01

    The aim of the work was the obtainment of polymeric matrices immobilized with crotoxin purified from crude venom of rattle snake. A matrix was processed by gamma irradiation by the grafting of a hydrogel onto a polymeric film which resulted in a copolymer for the chemical immobilization of crotoxin. The second matrix was attained by the entrapment of crotoxin in microspheres of epsilon-polycaprolactone. After the purification, the crotoxin proceeding from the snake Crotalus durissus terrificus was evaluated biochemical and biologically. The lethal dose (LD50%) of the toxin was 0.09/kg animal. The test of cytotoxicity not revealed any significant difference between the tumoral cells and the respective normal control cells in culture. Grafting copolymers were used as scaffold for the chemical immobilization of the purified crotoxin. For this purpose the low density polyethylene (LDPE) and the hydrophilic monomer 2-hydroxy-ethyl-methacrylate (HEMA) were copolymerized in a {sup 60}Co source. The copolymers (LDPE-g-PHEMA) showed grafting levels in the range of 2 and 50 %. In the infrared spectroscopy analysis (FTIR-ATR) it was observed in the copolymer, carbonyl groups (C=O) and hydroxyl groups -OH due to the grafting of PHEMA. The MEV micrographs showed a smooth surface for the virgin LDPE and a rough surface for the LDPE-g-PHEMA, owing to the presence of grafted PHEMA. The hydrophilicity was observed by the determination of water content in the copolymer after immersion in water. By the diffusion coefficient it was noted that from 30 % grafting degree, the copolymers become less hydrophilic due to the crosslinking increase among the chains in PHEMA. The biocompatibility of the LDPE-g-PHEMA was proved by the cytotoxicity test. At the end, the immobilized copolymer, the entrapped crotoxin and the free crotoxin was tested 'in vivo'. During 20 days, C3H strain mice were observed in their weight, behavior and motor changes. The results demonstrated that the group

  4. Biochemical and pharmacological studies of native and irradiated crotamine with gamma radiation of {sup 60}Co; Estudo bioquimico e farmacologico das crotaminas nativa e irradiada com radiacao gama de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Mitake, Malvina Boni

    2000-07-01

    Ionizing radiation can change the molecular structure and affect the biological properties of biomolecules. This has been employed to attenuate animal toxins. Crotamine is a strongly basic polypeptide from the South American rattlesnake venom, composed of 42 amino acid residues. It induces skeletal muscle spasms leading to a spastic paralysis of hind limbs in mice. The objective of this thesis was carry out biochemical and pharmacological studies of native and irradiated crotamine with {sup 60} Co. Crotamine was purified from Crotalus durissus terrificus venom by Sephadex G-100 gel filtration followed by ion exchange chromatography, using a Fast Performance Liquid Chromatography (FPLC) system. It was irradiated at 2 mg/ml in 0.15 M Na Cl with 2.0 kGy gamma radiation emitted by a {sup 60} Co source. The native and irradiated crotamine were evaluated by biochemical characterization, toxic activity (LD{sub 50} and biodistribution. The native and irradiated crotamine were labelled with 29.6 MBq of {sup 125} I using chloramine T method, and separated in a Sephadex G-50 column. Male Swiss mice (35{+-} 5 g), were injected i.p. with o.1 mL (2.4 x 10{sup 6} cpm/mouse) of {sup 125} I native crotamine or with 0.4 mL (1.3 x 10{sup 6} cpm/mouse) of {sup 125} I irradiated crotamine. At 0.08; 0.25; 0.5; 1; 2; 4; 8; 12 and 24 hours the animal were killed by ether inhalation. Blood, spleen, liver, kidneys, brain, lungs, heart, and skeletal muscle were collected in order to determine radioactivity content. The results showed that gamma radiation did not change the protein concentration, the electroforetic profile or the primary structure of the protein, although differences were shown by spectroscopic techniques. The gamma radiation diminished the toxicity of crotamine, but it did not abolish bioactivity. Biodistribution studies showed that native and irradiated crotamine have hepatic metabolism and renal elimination. The native and irradiated crotamine have affinity by skeletal

  5. Involvement of formyl peptide receptors in the stimulatory effect of crotoxin on macrophages co-cultivated with tumour cells.

    Science.gov (United States)

    Costa, E S; Faiad, O J; Landgraf, R G; Ferreira, A K; Brigatte, P; Curi, R; Cury, Y; Sampaio, S C

    2013-11-01

    Crotoxin (CTX) is the main neurotoxic component of Crotalus durissus terrificus snake venom. It inhibits tumour growth and modulates the function of macrophages, which are essential cells in the tumour microenvironment. The present study investigated the effect of CTX on the secretory activity of monocultured macrophages and macrophages co-cultivated with LLC-WRC 256 cells. The effect of the macrophage secretory activities on tumour cell proliferation was also evaluated. Macrophages pre-treated with CTX (0.3 μg/mL) for 2 h were co-cultivated with LLC-WRC 256 cells, and the secretory activity of the macrophages was determined after 12, 24 and 48 h. The co-cultivation of CTX-treated macrophages with the tumour cells caused a 20% reduction in tumour cell proliferation. The production of both H2O2 and NO was increased by 41% and 29% after 24 or 48 h of co-cultivation, respectively, compared to the values for the co-cultures of macrophages of control. The level of secreted IL-1β increased by 3.7- and 3.2-fold after 12 h and 24 h of co-cultivation, respectively. Moreover, an increased level of LXA4 (25%) was observed after 24 h of co-cultivation, and a 2.3- and 2.1-fold increased level of 15-epi-LXA4 was observed after 24 h and 48 h, respectively. Boc-2, a selective antagonist of formyl peptide receptors, blocked both the stimulatory effect of CTX on the macrophage secretory activity and the inhibitory effect of these cells on tumour cell proliferation. Taken together, these results indicate that CTX enhanced the secretory activity of macrophages, which may contribute to the antitumour activity of these cells, and that activation of formyl peptide receptors appears to play a major role in this effect.

  6. Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the North-East region of Argentina Consumo de fibrinógeno plasmático en ratones por acción de enzima con actividad trombínica presente en el veneno de cascabel del nordeste argentino

    Directory of Open Access Journals (Sweden)

    Silvana L. Maruñak

    2004-12-01

    Full Text Available Due to variability of venom components from the same species of snakes that inhabit different regions, particular properties of the venom of Crotalus durissus terrificus that inhabits the North-East of Argentina were studied. Gyroxin, a thrombin-like enzyme, was isolated from this venom by gel filtration and affinity chromatography, it was found to be homogeneous according to SDS-PAGE, with a molecular weight of 33 kDa. "Gyroxin syndrome" in mice was tested and it showed changes in the animal behavior, confirming that the isolated thrombin-like enzyme is gyroxin. Effects of this enzyme and the crude venom on mice plasmatic fibrinogen levels were determined. The mice plasma fibrinogen decreased rapidly until incoagulability during the first hour after thrombin-Iike enzyme injection, then reaching its normal level 10 hours after injection; whereas crude venom resulted in a 60% decrease of the mice plasma fibrinogen, reaching its normal level after the same period of time. After 1 hour of gyroxin inoculation, intravascular coagulation was observed in histological cuttings of lung, cardiac muscle and liver. The isolated enzyme showed strong hydrolyzing activity on fibrinogen and fibrin in vitro, whereas the crude venom exhibited weak hydrolyzing activity on both substrates. It is probable that this very low activity is due to the low percentage of the enzyme in the crude venom. Decreasing of plasmatic fibrinogen levels may be due to either the coagulant or hydrolyzing actions of the enzyme.Teniendo en cuenta la variabilidad de los componentes del veneno de serpientes de una misma especie que habitan regiones diferentes, se decidió estudiar las propiedades particulares del veneno de Crotalus durissus terrificus que habita el nordeste de Argentina, Giroxina, una enzima con actividad trombínica, fue aislada del veneno por cromatografía de filtración por gel y de afinidad; se comprobó su homogeneidad y se determinó su peso molecular, 33 kDa, por

  7. [First record of Zoophthora radicans (Entomophthorales: Entomophthoraceae) on adults of the Paraguay tea psyllid, Gyropsylla spegazziniana Lizer & Trelles (Hemiptera: Psyllidae), in Brazil].

    Science.gov (United States)

    Alves, Luis F A; Leite, Luis G; Oliveira, Daian G P de

    2009-01-01

    We report the first occurrence of Zoophthora radicans infecting adults of Gyropsylla spegazziniana Lizer & Trelles in a commercial Paraguay tea plantation (Ilex paraguariensis), in Cascavel, PR, Brazil. The fungus prevalence was high (90% of mortality), considered a natural epizooty.

  8. Health Data Concerning Ecuador

    Science.gov (United States)

    1960-01-27

    publishes the Bolletin de Miedicina y Cirugia - The Ecuadorian Society of Pediatrics sponsors extension courses and lectures for physicians. The MedicaI...serum terrificus snake) cascabel Lachesis rnuta Bushmaster Hernatoxin + Antilaquetic serum proteolysin . oral snakes Ylicrurus (Elaps) Coralilla

  9. Study of crotoxin mechanism of action to mammary carcinomas and evaluation of its potential as a radiopharmaceutical; Estudo do mecanismo de acao da crotoxina em tumores mamarios e avaliacao do seu potenctial radiofarmaceutico

    Energy Technology Data Exchange (ETDEWEB)

    Silveira, Marina Bicalho

    2010-07-01

    Crotoxin, the main component of Crotalus durissus terrificus snake venom, has been studied since 1938. It is a natural polypeptidic complex with pharmacological potential because of its antitumoral properties which has attracted great interest for diagnosis and therapy of oncological diseases. However, Crotoxin mechanism of action and sites of specific interaction on tumor cells are still misunderstood. Breast cancer is the second most frequent type in the world and the most common cancer in women. About 30 to 60% of mammary tumors overexpress epidermal growth factor receptor (EGFR), a transmembrane protein related to cell proliferation. Since literature has reported that Crotoxin antitumoral effect is more potent on cells with EGFR overexpression the objectives of this work were to evaluate Crotoxin cytotoxic effects on mammary tumor cells human breast carcinoma (MCF-7) and Ehrlich tumor cells (murine ascitics carcinoma), and to investigate the specific molecular interaction of Crotoxin on Ehrlich tumor cells. Initially, Crotoxin was radiolabelled with iodine-125 ({sup 125}I-Crotoxin) and iodine-131 ({sup 131}I-Crotoxin). Saturation and competition assay were carried out to characterize Crotoxin in vitro interaction; Crotoxin biodistribution studies and singlephoton emission computed tomography (SPECT) of mice bearing Ehrlich tumor have been evaluated to describe in vivo interaction. Our results showed that Crotoxin presented cytotoxic effect against Ehrlich with DL{sub 50} in vitro (concentration of compound which is lethal for 50% of cells) of about one micromolar, but did not present significant effect against MCF-7. Morphological alterations characteristic of apoptosis suggests programmed cell death. {sup 125}I-Crotoxin interaction with Ehrlich tumor cells was saturable with approximately 70% specificity, and presented K{sub d}=24.98 nmol/L and B{sub max}=16,570 sites/cell for low affinity binding sites and K{sub d}=0.06 nmol/L and B{sub max}=210 sites

  10. Energy inputs and outputs in the wheat production at different localizations; Balanco de energia na producao de trigo em diferentes locais

    Energy Technology Data Exchange (ETDEWEB)

    Gollmann, Pedro; Greco, Marcelo; Campos, Alessandro Torres [Universidade Estadual do Oeste do Parana (UNIOESTE), Marechal Candido Rondon, PR (Brazil). Grupo de Pesquisas em Ambiencia do Oeste do Parana], e-mail: jefersonklein@yahoo.com.br; Klein, Jeferson [Universidade Estadual do Oeste do Parana (UNIOESTE), Marechal Candido Rondon, PR (Brazil); Palagi, Celso A.; Jurach, Jorge J. [Cooperativa Central de Pesquisa Agricola Tecnologia da Nossa Terra (COODETEC), Cascavel, PR (Brazil)

    2004-07-01

    The intensifying and modernization of agriculture, in spite of improving the productivity, it caused a complete petroleum derived dependence, so much in the form of fuels, lubricants and machinery, as in the form of inputs for the production. This work had as objective to esteem the energy inputs and outputs and the energy conversion, in two different local, in the west of Parana State/Brazil. The largest consumption was observed for the direct energy category, with 95.6% and 95.28% in Cascavel and Palotina, respectively. In the direct energy flow, the Palotina production spent 5,0% more. The consumption of fossil energy was of 22.32% for the Palotina farm and of 23.84% for the Cascavel farm. The energy conversion was of 3.2 and 1.8, for the wheat productions in Cascavel and Palotina, respectively. (author)

  11. Descrição da larva de último estádio de Micrathyria pseudeximia Westfall (Odonata, Libellulidae

    Directory of Open Access Journals (Sweden)

    Marina Schmidt Dalzochio

    2009-08-01

    Full Text Available A larva de último estádio de Micrathyria pseudeximia Westfall, 1992 é descrita e ilustrada a partir de material procedente de Cascavel, Paraná, Brasil. A caracterização do gênero com base nas larvas é ampliada.Description of the Ultimate Stadium Larva of Micrathyria pseudeximia Westfall(Odonata, Libellulidae.Abstract. The larva of ultimate stadium of Micrathyria pseudeximia Westfall, 1992 is described and illustrated based on material collected in Cascavel Municipality, Paraná State, Brazil. The generic concept based on the larvae is amplified.

  12. Scenedesmaceae (Chlorophyceae – Chlorococcales) de um lago artificial urbano: Desmodesmus e Scenedesmus - DOI: 10.4025/actascibiolsci.v29i3.483 Scenedesmaceae (Chlorophyceae – Chlorococcales) of an urban artificial lake: Desmodesmus and Scenedesmus - DOI: 10.4025/actascibiolsci.v29i3.483

    OpenAIRE

    Norma Catarina Bueno; Carina Moresco

    2007-01-01

    Trata-se do inventário florístico de Scenedesmaceae no Lago Municipal de Cascavel (24°82’S, 53º28´W), Paraná, Brasil. As amostras foram coletadas na região litorânea, no período de janeiro de 2002 a outubro de 2003. Foram registrados 21 táxons, sendo 13 do gênero Desmodesmus e oito pertencentes ao gênero Scenedesmus. O trabalho apresenta descrição, chave e ilustrações para a identificação das espécies.An inventory of Scenedesmaceae was made in the Lago Municipal de Cascavel (24°82’S, 53º 28´W...

  13. RÁDIO COMUNITÁRIA NA TRÍPLICE FRONTEIRA : participação étnica e de gênero na Rádio Norte FM

    OpenAIRE

    Inês Amarante, Maria; Inés Varela, Sonia

    2016-01-01

    International audience; This article presents part of the research “Community Radiophonic in the Triple Border: gender, ethnicity and social inclusion media”, whose proposal is a thorough study of this groups participation in community radio stations, in the regions of the borders, between the State of Parana, Paraguay and Argentina and how these vehicles have favored the communicative citizenship. Highlighting the case of Radio North FM, in the city of Cascavel, western region of Parana. Cur...

  14. Allelopathy of Camelina sativa Boiss. (Brassicaceae) on germination and early development of Bidens pilosa (L.) and Glycine max (L.) Merr

    OpenAIRE

    Jéssica da Silva; Andréa Maria Teixeira Fortes; Fernanda Melo Gomes; Tassiane Terezinha Pinto; Thaliny Bonamigo; Nayara Parisoto Boiago

    2011-01-01

    This study evaluated the possible presence of allelopathic potential in camelina, as well as its effect on germination and early growth of soybean seedlings and beggarticks, in order, to verify the possibility of cultivating it with soybeans in a culture rotation system and its use as a herbicide. The experiments were carried out at the Laboratory of Plant Physiology of Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil. The design was completely randomized and the evaluations...

  15. Radiographic evaluation of the effect of obesity on alveolar bone in rats with ligature-induced periodontal disease

    Directory of Open Access Journals (Sweden)

    do Nascimento CM

    2013-10-01

    Full Text Available Cassiane Merigo do Nascimento,1 Tiago Cassol,2 Fernanda Soares da Silva,3 Maria Lucia Bonfleur,4 Carlos Augusto Nassar,5 Patricia Oehlmeyer Nassar5 1Biologica Science and Health Center, State University of West Paraná (UNIOESTE, Cascavel, Paraná, Brazil; 2State University of West Paraná (UNIOESTE, Cascavel, Paraná, Brazil; Department of 3Pharmacy, 4Fisiology, 5Periodontology, Dental School, State University of West Paraná (UNIOESTE, Cascavel, Paraná, Brazil Abstract: There is evidence that the lack of metabolic control of obese patients may accelerate periodontitis. The aim of this study was to evaluate radiographically the effect of cafeteria-diet-induced obesity on alveolar bone loss in rats subjected to periodontal disease. Twenty male Wistar rats were randomly divided into four groups: 1 control group, 2 control and ligature group; 3 cafeteria group; and 4 cafeteria and ligature group. The animals were evaluated for obesity and euthanized, and the mandible of each rat was removed to perform a radiographic evaluation of alveolar bone loss and its effect on diet-induced obesity. The results showed greater alveolar bone loss in the mice in Group 4 (P<0.01. Thus, we concluded that obese mice, on average, showed greater radiographic evidence of alveolar bone loss than mice undergoing induction of obesity. Keywords: periodontal disease, radiography, obesity

  16. Morfologia de Osteodermos de Crocodilomorfos do Sítio Paleontológico de Peirópolis (Bacia Bauru, Cretáceo Superior.

    Directory of Open Access Journals (Sweden)

    Thiago da Silva Marinho

    2006-07-01

    Full Text Available Osteoderms are dermal bony plates situated over the epiderm andcovered by queratin layer. Until now are recognized at the Paleontological Site of Peirópolis three distinct crocodylomorph species, the Peirosauridae Peirosaurus termini Price, 1955 and Uberabasuchus terrificus Carvalho; Ribeiro & Ávilla, 2004 and the Trematochampsidae Itasuchus jesuinoi Price, 1955. The peirosaurids have nostrils frontally positioned within the skull and are regarded as terrestrial crocodylomorphs. The trematochampsids differentlyfrom the peirosaurids have the nostrils positioned at the dorsal portion of the skull, similar to the extant crocodiles with aquatic habits. The osteoderms morphological analysis of P. tormini, U. terrificus and I. jesuinoi corroborate, with the cranial evidences, for the terrestrial or aquatic lifestyle of these animals.

  17. [Women's body language during the post-partum period: language understanding based on the work of Maurice Merleau-Ponty].

    Science.gov (United States)

    Machineski, Gicelle Galvan; Schneider, Jacó Fernando; Bastos, Carmen Célia Barradas Correia

    2006-09-01

    This study aimed understanding the woman's language during the post-partum period as to her perceptions of her body language. This study was based in the work of Maurice Merleau-Pont. Ten women living in Cascavel, State of Parand, Brazil, were interviewed from February to May 2005. This study allowed the understanding of the meaning that the woman gives to her existence in the post childbirth period. This study may support training of health professionals in terms of understanding how women experience the post-partum period, thereby allowing better care of these patients.

  18. "Meninos do Cense" : as relações de estigmatização, violência e disciplinarização de adolescentes em conflito com a lei internados

    OpenAIRE

    Raquel Ribeiro Moreira

    2011-01-01

    Esta tese tem como objetivo de pesquisa os modos de determinação e de tratamento de adolescentes em conflito com a lei internados. Para isso, escolhemos o CENSE II de Cascavel, instituição considerada modelar na América Latina no tratamento de jovens em cumprimento de medida socioeducativa, como nosso “lugar de investigação”. Tomamos, para isso, a Análise de Discurso de linha francesa, fundada por Michel Pêcheux, como sustentação teórica de nossas análises. Como corpus, trabalhamos mais espec...

  19. Workplace health promotion: analysis of actions proposed by graduates of a training course (2012

    Directory of Open Access Journals (Sweden)

    Francielle Bertusso

    2014-12-01

    Full Text Available The incorporation of the workers knowledge in the actions of health promotion and prevention can strengthen the search for better working conditions and the protection of workers' health. This bibliographic study aimed to analyze, from the theoretical framework of occupational health, the actions proposed by graduates of a training course for health promotion for healthcare workers in 2012. Of the 221 projects analyzed, 1Enfermeira, aluna especial do Programa de Mestrado em Biociências e Saúde da Universidade Estadual do Oeste do Paraná, campus de Cascavel. E-mail: franbertusso@hotmail.com 2 Doutora em Saúde Coletiva, Professora adjunta do Curso de Enfermagem do Centro de Ciências Biológicas e da Saúde da Universidade Estadual do Oeste do Paraná, campus de Cascavel, Pr. 3 Enfermeiro da Secretaria de Estado da Saúde do Paraná - SESA. Aluno especial do Programa de Mestrado em Biociências e Saúde da Universidade Estadual do Oeste do Paraná, Campus de Cascavel, Pr. 4 Doutora em Enfermargem pela Universidade de São Paulo. Professora do Programa de Mestrado em Biociências e Saúde do Centro de Ciências Biológicas e da Saúde da Universidade Estadual do Oeste do Paraná, campus de Cascavel health workers were the priority target audience (84%, as well as managers (12%, from various regions of the country with a predominance of proposals from the southeast region (107 projects and northeast region (55 projects, and in several workplace activities. Program proposals attempted to solve or minimize three sets of problems: a related to the work environment, b labor management and c the workers' health. Examination of the proposals pointed to a working reality permeated by incidents of accidents by exposure to biological material, ionizing radiation, needlestick and sharps injuries, and injuries related to musculoskeletal overload, mental health and mental and behavioral disorder such as depression, alcoholism, stress, burnout and moral

  20. Global solar irradiation in function of the insolation reason; Irradiacao solar global em funcao da razao de insolacao

    Energy Technology Data Exchange (ETDEWEB)

    Recieri, Reinaldo Prandini; Ferruzzi, Yuri; Silva, Suedemio de Lima [Universidade Estadual do Oeste do Parana (UNIOESTE), Marechal Candido Rondon, PR (Brazil). Fac. de Agronomia; Quallio, Silvana [Universidade Estadual do Oeste do Parana (UNIOESTE), PR (Brazil). Fac. de Biologia; Batista, Vitor Roberto Lourenco [Universidade Estadual do Oeste do Parana (UNIOESTE), PR (Brazil). Fac. de Engenharia Eletrica

    2004-07-01

    The present work shows the reason (K{sub t}=R{sub G}/R{sub o}) of global solar irradiation for the irradiation in the top of the atmosphere in the horizontal incidence in function of the insolation reason (n/N), objectifying itself to find an equation esteem the daily global solar irradiation in the region of Cascavel/PR. The experimental data had been gotten in Station Agrometeorological of the IAPAR (Agronomic Institute of the Parana) mounted in the Coodetec/Cascavel-PR (Latitude 24,89 deg S, Longitude 53,40 deg O and altitude of 682m). The component of global the solar irradiation was monitored by an actinograph and the hour of solar brightness (n) through a heliograph (R. FUESS Berlin-stegutz) in a period of 17 years, of which four had been used in the validation of the model. The distribution of the daily values (K{sub t} x n/N), related through simple linear regression, supplied an equation of R{sub G}/R{sub o}=0,16+0,40n/N estimate with raised determination coefficient (R{sup 2} = 88.89%), in the validation of the model got a -9,76% shunting line varying 11,44% with average error in the 0,84% order, these statistical values shows that global the solar irradiation, in the region of Rattlesnake, can be esteem with good precision. (author)

  1. Scenedesmaceae (Chlorophyceae – Chlorococcales de um lago artificial urbano: Desmodesmus e Scenedesmus = Scenedesmaceae (Chlorophyceae – Chlorococcales of an urban artificial lake: Desmodesmus and Scenedesmus

    Directory of Open Access Journals (Sweden)

    Norma Catarina Bueno

    2007-07-01

    Full Text Available Trata-se do inventário florístico de Scenedesmaceae no Lago Municipal de Cascavel (24°82’S, 53º28´W, Paraná, Brasil. As amostras foram coletadas na região litorânea, no período de janeiro de 2002 a outubro de 2003. Foram registrados 21 táxons, sendo 13 do gênero Desmodesmus e oito pertencentes ao gênero Scenedesmus. O trabalhoapresenta descrição, chave e ilustrações para a identificação das espécies.An inventory of Scenedesmaceae was made in the Lago Municipal de Cascavel (24°82’S, 53º 28´W, Paraná, Brazil. The samples were collected in littoral zone, from January 2002 to October 2003. Twenty-one taxa were registered, 13 belong to Desmodesmus and eight to Scenedesmus genera. This paper presents description, key and illustrations to identify species.

  2. Scenedesmaceae (Chlorophyceae – Chlorococcales de um lago artificial urbano: Desmodesmus e Scenedesmus - DOI: 10.4025/actascibiolsci.v29i3.483 Scenedesmaceae (Chlorophyceae – Chlorococcales of an urban artificial lake: Desmodesmus and Scenedesmus - DOI: 10.4025/actascibiolsci.v29i3.483

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    Norma Catarina Bueno

    2007-12-01

    Full Text Available Trata-se do inventário florístico de Scenedesmaceae no Lago Municipal de Cascavel (24°82’S, 53º28´W, Paraná, Brasil. As amostras foram coletadas na região litorânea, no período de janeiro de 2002 a outubro de 2003. Foram registrados 21 táxons, sendo 13 do gênero Desmodesmus e oito pertencentes ao gênero Scenedesmus. O trabalho apresenta descrição, chave e ilustrações para a identificação das espécies.An inventory of Scenedesmaceae was made in the Lago Municipal de Cascavel (24°82’S, 53º 28´W, Paraná, Brazil. The samples were collected in littoral zone, from January 2002 to October 2003. Twenty-one taxa were registered, 13 belong to Desmodesmus and eight to Scenedesmus genera. This paper presents description.

  3. Effect of suramin on myotoxicity of some crotalid snake venoms

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    E.Z. Arruda

    2002-06-01

    Full Text Available We investigated the protective effect of suramin, an enzyme inhibitor and an uncoupler of G protein from receptors, on the myotoxic activity in mice of different crotalid snake venoms (A.c. laticinctus, C.v. viridis, C.d. terrificus, B. jararacussu, B. moojeni, B. alternatus, B. jararaca, L. muta. Myotoxicity was evaluated in vivo by injecting im the venoms (0.5 or 1.0 mg/kg dissolved in physiological saline solution (0.1 ml and measuring plasma creatine kinase (CK activity. Two experimental approaches were used in mice (N = 5 for each group. In protocol A, 1 mg of each venom was incubated with 1.0 mg suramin (15 min, 37ºC, in vitro, and then injected im into the mice at a dose of 1.0 mg/kg (in vivo. In protocol B, venoms, 1.0 mg/kg, were injected im 15 min prior to suramin (1.0 mg/kg, iv. Before and 2 h after the im injection blood was collected by orbital puncture. Plasma was separated and stored at 4ºC for determination of CK activity using a diagnostic kit from Sigma. Preincubation of some venoms (C.v. viridis, A.c. laticinctus, C.d. terrificus and B. jararacussu with suramin reduced (37-76% the increase in plasma CK, except for B. alternatus, B. jararaca or L. muta venoms. Injection of suramin after the venom partially protected (34-51% against the myotoxicity of B. jararacussu, A.c. laticinctus and C.d. terrificus venom, and did not protect against C.v. viridis, L. muta, B. moojeni, B. alternatus or B. jararaca venoms. These results show that suramin has an antimyotoxic effect against some, but not all the North and South American crotalid snake venoms studied here.

  4. Incidência e aspectos clínico-laboratoriais do Lúpus eritematoso sistêmico em cidade do Sul do Brasil Incidence and clinical-laboratory aspects of systemic lupus erythematosus in a Southern brazilian city

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    Carlos Alberto Kenji Nakashima

    2011-06-01

    Full Text Available INTRODUÇÃO: Estudos epidemiológicos brasileiros sobre o lúpus eritematoso sistêmico (LES são bastante escassos e os dados existentes hoje são praticamente todos de literatura internacional. OBJETIVOS: Determinar a incidência e algumas características clínicas e laboratoriais de pacientes com LES em Cascavel, Paraná - Brasil. PACIENTES E MÉTODOS: Os dados foram coletados entre agosto de 2007 e julho de 2008 em todos os serviços de saúde do município que possuíam atendimentos na especialidade de Reumatologia: um hospital universitário, um ambulatório público e três clínicas privadas da cidade. RESULTADOS: Foram identificados 14 pacientes com diagnóstico de LES, resultando em uma incidência estimada de 4,8 casos/100.000 habitantes/ano. Todos os pacientes eram do sexo feminino, com média de idade de 41,5 anos. A faixa etária com maior incidência foi a de 30 - 39 anos e 92,8% apresentaram quatro ou mais dos 11 critérios do American College of Rheumatology (ACR para o diagnóstico de LES. O tratamento farmacológico dos pacientes também foi avaliado e mostrou estar de acordo com o Consenso Brasileiro para o tratamento de LES. CONCLUSÃO: A incidência obtida em Cascavel/PR está próxima das incidências observadas em estudos internacionais.INTRODUCTION: Brazilian epidemiological studies on systemic lupus erythematosus (SLE are scarce, and currently available data originate almost entirely from international literature. OBJECTIVES: To determine the incidence and some clinical and laboratory characteristics of patients with SLE in the municipality of Cascavel, state of Paraná, Brazil. PATIENTS AND METHODS: Data were collected from August 2007 to July 2008 in all health services of Cascavel providing health care in rheumatology: a university-affiliated hospital, a public outpatient clinic, and three private clinics. RESULTS: The study identified 14 patients diagnosed with SLE, which resulted in an estimated incidence of 4

  5. The Late Cretaceous fauna and flora of the Uberaba area (Minas Gerais State, Brazil)

    Science.gov (United States)

    Candeiro, Carlos Roberto A.; Santos, Adriano R.; Bergqvist, Lílian P.; Ribeiro, Luiz Carlos B.; Apesteguía, Sebastián

    2008-03-01

    The Uberaba area, in Minas Gerais State, Brazil, yields a rich continental fauna and flora from the Late Cretaceous Uberaba and Marília formations. This paper reviews the diversity of the biota recorded from these formations. The most significant taxa from Peirópolis are the frog Baurubatrachus pricei, the turtle Cambaremys langertoni, the lizard Pristiguana brasiliensis, the crocodyliforms Itasuchus jesuinoi, Peirosaurus tormini and Uberabasuchus terrificus, the titanosaurian Baurutitan britoi, Trigonosaurus pricei, Aeolosaurus sp., indeterminate titanosaurians, and abelisaurid, carcharodontosaurid and maniraptoran theropods. Together with faunas of a similar age in Argentina and Madagascar, the assemblages contribute to a better understanding of Late Cretaceous Gondwanan faunas as a whole.

  6. Contribuição à biologia de serpentes da Bahia, Brasil: I. vivíparas Contribution to reproductive biology of snakes in Bahia, Brazil: I. viviparous

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    Rejane Maria Lira-da-Silva

    1994-01-01

    Full Text Available Great part of lhe avaiable data about snakes reprodution refers to species coming from subtropical and temperate regions. In Brazil, the data is rather rare and can be found in various works where information is restricted. Results from studies developed with five viviparous snakes - Crotalus durissus cascavella (Wagler, 1824. Bothrops erythromelas(Amaral, 1923, B. leucurus (Wagler, 1824, Helicops leopardinus (Schlegel, 1873 and Thamnodynastes strigilis (Thiinberg, 1787 - which come from the Northeast of Brazil (Bahia are described. Data about pregnancy and birth, number, sex ratio, length and weight of neonates is given and discussed.

  7. Structural studies of the oligomeric formation crotoxin and their complexes; Estudos estruturais da formacao oligomerica da crotoxina e seus complexos

    Energy Technology Data Exchange (ETDEWEB)

    Morelli, Humberto A.S.; Fernandes, Carlos A.H.; Magro, Angelo J.; Fontes, Marcos R.M.; Fernandez, Roberto M., E-mail: rmorato@ibb.unesp.br [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Inst. de Biociencias. Dept. de Fisica e Biofisica; Bicev, Renata N.; Oliveira, Cristiano L.P. de [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Inst. de Fisica. Dept. de Fisica Experimental; Barioni, Marina B.; Ito, Amando S. [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Fac. de Filosofia, Ciencias e Letras. Dept. de Fisica

    2013-08-15

    The crotoxin is a phospholipase A{sub 2} that is the mainly responsible for the neurotoxic effect of the snakes' venom of the species Crotalus durissus, popularly known as rattlesnakes. In the crotoxin there are two subunits: an acidic, non-toxic and devoid of enzymatic action, (called crotoxin A or CA); and a basic, toxic with enzymatic activity (called crotoxin B or CB). The main objective of this work is to study the CA, CB and the complex CA + CB using the Small-Angle X-ray Scattering (SAXS), Optical absorption spectrometry and static fluorescence techniques. (author)

  8. Ação de venenos de serpentes brasileiras sôbre a alexina ou complemento

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    F. da Rocha Lagôa

    1947-06-01

    Full Text Available O autor estudou a ação inativante, sôbre o complemento de cobaia, alguns venenos de serpentes brasileiras pertencentes às famílias dos Elapideos e Crotalideos. Da primeira, foi utilizado veneno de Micrurus frontalis, da segunda, foram usados venenos de espécies pertencentes aos gêneros Crotalus (C. terrificus e Bothrops (B. atrox, B. neuwiedii, B. jararaca, B. ja-raracussú, B. cotiara e B. alternata. O venenos de M. frontalis e C. terri¬ficus se revelaram incapazes de inativar o complemento, ao passo que os diversos de Bothrops empregados se mostraram altamente inativantes, destruindo sempre o 4.° componente do complemento (C4, fração idêntica à afetada pela ação da amônea.The author shows in this paper the results of the inactivation of com¬plement or alexin by some Brazilian snakes venoms of the Elapideae and Cro¬talideae families. The venom of Micrurus frontalis (Elapideae family and of Crotalus terrificus (Crotalideae family did not destroy the complement; but the venoms of Bothrops generus (B. atrox, B. neuwiedii, B. jararaca, B. jararacussú, B. cotiara e B. alternata, (Crotalideae family are highly active in the inactivation complement, always destroing the fourth component, whichis exactly the same component that is detroyed by ammonia.

  9. A percepção dos profissionais sobre a assistência ao parto domiciliar planejado La percepción de los profesionales acerca de la atención al parto domiciliario planeado The perception of professionals regarding planned home birth

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    Tatianne Cavalcanti Frank

    2013-03-01

    Full Text Available Estudo qualitativo com objetivo de compreender a percepção dos profissionais no acompanhamento do parto domiciliar planejado. Foram entrevistados oito profissionais que atuaram em partos domiciliares, em Cascavel/PR. A análise revelou que o domicílio, enquanto local de assistência, possibilita o protagonismo da mulher e da família pela tranquilidade, calma e autonomia. O ambiente é seguro quando se segue requisitos como baixo risco gestacional, avaliação adequada, no decorrer da evolução do parto, presença de materiais adequados, rede transdisciplinar e local pré-definido para encaminhamentos. Os profissionais apontam, ainda, como fundamental, a participação familiar no processo. Conclui-se que o parto domiciliar é uma excelente estratégia para transformar e melhorar a qualidade da atenção obstétrica.Estudio cualitativo con el objetivo de comprender la percepción de los profesionales en el acompañamiento del parto domiciliario planeado. Fueron entrevistados ocho profesionales que trabajaron en los partos domiciliarios en Cascavel/PR. El análisis reveló que el domicilio como un lugar de atención posibilita el protagonismo de la mujer y de la familia por la tranquilidad, calma y autonomía. El ambiente es seguro cuando se siguen los requisitos tales como bajo riesgo gestacional, evaluación adecuada de la evolución del parto, presencia de materiales adecuados, red transdisciplinaria y local predefinido para encaminamientos. Los profesionales apuntan también como fundamental la participación familiar en el proceso. Se concluyó que el parto domiciliario es una excelente estrategia para transformar y mejorar la calidad de la atención obstétrica.This is a qualitative study aimed to understand the perception of professionals regarding planned home birth. Eight professionals who took part in home births in Cascavel/PR were interviewed. The analysis revealed that home, as the care place, allows more prominence to women and

  10. Acompanhamento de enfermagem em saúde pública, assessoramento pedagógico e agrícola numa comunidade de assentados sem-terra na zona rural: relato de experiência Acompañamiento de enfermería en salud pública, asesoramiento pedagógico y agrícola en una comunidad de asentados "sin tierra" en la zona rural: relato de experiencia Public health nursing, pedagogical and agricultural ad vice in a rural community of settled landless people: an experience

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    Jacó Fernando Schneider

    1995-01-01

    Full Text Available O estudo relata uma experiência no acompanhamento de Enfermagem, assessoramento pedagógico e agrícola desenvolvido em uma comunidade de assentados sem-terra na zona rural da cidade de Cascavel, estado do Paraná, que teve como premissa básica a integração docente/discente, oferecendo oportunidade de socialização do saber, integração teoria e prática e contato direto com a comunidade. Aponta para a necessidade da enfermagem compreender melhor este tipo de realidade buscando, através da observação e vivência, uma visão crítica com possibilidades de intervenção.El estudio relata una experiencia en el acompañamiento de enfermería, asesoramiento pedagógico y agrícola desarrollado en una comunidad de asentados "sin tierra" en la zona rural perteneciente al municipio de Cascavel, estado del Paraná, que tuvo como principio básico la integración docente/alumno, ofreciendo oportunidad de socialización del saber, integración teoría y práctica y contacto directo con la comunidad. Señala la necessidad de la enfermería comprender mejor este tipo de realidad, buscando por medio de la observación y vivencia, una visión crítica con posibilidades de intervención.This study reports an experience in nursing accompaniment, pedagogical and agricultural advice in a community of settled people without land in the countryside of Cascavel, state of Paraná. The basic premise was student/teacher integration offering the opportunity of knowledge socialization, integration of theory and practice and direct contact with the community. It points out that nursing needs a better understanding of this type of reality seeking, by observation and living, a critical vision with the possibility of intervention.

  11. [Toxicity of venoms from snakes of medical importance in México].

    Science.gov (United States)

    de Roodt, Adolfo R; Estévez-Ramírez, Judith; Paniagua-Solís, Jorge F; Litwin, Silvana; Carvajal-Saucedo, Alejandro; Dolab, Jorge A; Robles-Ortiz, Luis E; Alagón, Alejandro

    2005-01-01

    The characterization of the toxic activities of snake venoms is necessary to understand the physiopathology of the envenomation and to test the potency of the antivenoms used to treat this pathology. Because of the lack of data on the toxic activities of venoms from Mexican snakes of medical importance, we studied the venoms from Bothrops asper, Athropoides nummifrr, Agkistrodon billineatus, Crotalus durissus durissus, Crotalus basiliscus, Crotalus scutulatus, Crotalus atrox and Micrurus nigrocinctus. The studies performed were: SDS-PAOE, determination of lethal potency, hemorrhagic, necrotizing, coagulation on plasma and fibrinogen, phospholipasic and fibri(noge)nolytic activities. In addition we studied the neutralizing capacity of the toxic activities of an antivenom currently used for the treatment of snakebites in Mexico. The venom from viperids showed important hemorrhagic, necrotizing, coagulative on plasma, prothrombinic, fibrinogenolytic and phospholipase activities. The venoms with the highest lethal potency were those of Micrurus nigrocinctus and Crotalus scutulatus; however, the viperine venom that globally displayed the most potent toxic activities was from Bothrops asper. All the venoms showed toxic activities of similar range to those described for other American venomous snakes. The activity on plasma or fibrinogen varied widely among the different venoms but all displayed capacity to act on the coagulation system. The antivenom tested not only neutralized the lethality B. asper venom but also its other toxic activities.

  12. The Defense of Socio-Educational Internment: Feature of the Hygienist Principles

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    Juliana Biazze Feitosa

    2014-04-01

    Full Text Available Youth violence and interventions to affront it are the focus of our scientific research. The aim of this study is evaluate the existence of the aspects contained in the justifications of hygienism judgments of teenagers who meet admission youth work. In this sense, we 21 sentences that determined the internment and its supporting materials which makes up the judicial process of teenagers that have entered the year 2010 in the Center of Socio-education II in Cascavel Paraná State. The results led us to conclude that nowadays, notwithstanding the Children and Adolescent, we are still, by judicial determination, removing the Teen Delinquency situation and closed the judicial term returning it to the same context from which it was withdrawn. Enshrined due differences in shape and time note this routing aspects of the ideology of mental hygiene and the minor doctrine.

  13. THE ISSUE OF EDUCATION IN MARX: SOME NOTES

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    João Carlos da Silva

    2011-02-01

    Full Text Available : Article as a result of readability and reflections carried out in the study group on the works of Marx and Engels, within the group of search HISTEDOPR - GT, Cascavel, PR. The question educational not received a specific treatment in the work of Marx, even if many seek in their formulations, theoreticalmethodological principles for the process of teaching-learning. Its educational thinking must be observed in the whole production marxist. This text to understand, in the light of historical change, the question educational, based on some of his writings. Their conception of education is immersed in horizon of socioeconomic relations, central object of discussion. We will see that education, in Marx, presents a politicalideological perspective, even before the determinant economic. Understand the analysis of Marx about the educative phenomenon does without to observe its way to understand the society.

  14. Enteroparasitos em materiais fecal e subungueal de manipuladores de alimentos, Estado do Paraná, Brasil - DOI: 10.4025/actascihealthsci.v31i2.4935 Enteroparasites in fecal and subungual matter from food handlers, Parana State, Brazil- DOI: 10.4025/actascihealthsci.v31i2.4935

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    Dina Lúcia Moraes Falavigna

    2009-09-01

    Full Text Available Este estudo indica a presença de enteroparasitos em material fecal e subungueal de 343 manipuladores de alimentos de Cascavel, Estado do Paraná, Brasil, de ambos os gêneros e com idade entre 14 e 75 anos. O material fecal foi analisado pelos métodos de Lutz, Ritchie modificado e Ziehl-Neelsen modificado e o subungueal, pelo Ritchie. O material fecal foi positivo para 131 (38,2% manipuladores. As espécies mais prevalentes foram Endolimax nana (67,9%, Entamoeba coli (35,9%, Blastocystis sp. (28,2%, Entamoeba histolytica/díspar (10,1% e Giardia duodenalis (8,4%, e E. nana foi diferente (p = 0,00 em relação às demais. A infecção por protozoários foi maior do que por helmintos (p = 0,00. Foi verificada associação entre a positividade para algum parasito e o gênero masculino e a categoria ocupacional (p ≤ 0,05. O material subungueal foi positivo em 17 indivíduos (5,0%, com encontro de E. nana (2,9%, E. coli (1,2%, G. duodenalis (0,3% e a associação de E. nana e E. coli (0,6%, não havendo convergência com o material fecal (p = 0,00. A ocorrência de enteroparasitos nos manipuladores de alimentos de Cascavel, Estado do Paraná é alta, indicando condições higiênicas inadequadas e a necessidade de medidas educativas rígidas, para se evitar que agentes patogênicos para o homem sejam propagados para os alimentos pela manipulação.This study shows the presence de enteroparasites in fecal and subungual material of 343 food handlers in the city of Cascavel, Paraná State, Brazil, from both genders and ages between 14 and 75 years. Fecal samples were analyzed using Lutz, modified Ritchie and Ziehl-Neelsen techniques, and subungual material was analyzed using the Ritchie method. Fecal samples were positive in 131 (38.2% handlers. Species with highest prevalence were Endolimax nana (67.9%, Entamoeba coli (35.9%, Blastocystis sp. (28.2%, Entamoeba histolytica/dispar (10.1% and Giardia duodenalis (8.4%, with E. nana being significantly

  15. Deficiência de ferro, prevalência de anemia e fatores associados em crianças de creches públicas do oeste do Paraná, Brasil Iron deficiency and prevalence of anemia and associated factors in children attending public daycare centers in western Paraná, Brazil

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    Valdete Carreira Rodrigues

    2011-06-01

    Full Text Available OBJETIVO: Avaliar o estado nutricional de ferro, a prevalência de anemia e fatores associados, em crianças de 6 a 24 meses frequentadoras de creche pública em Cascavel, Região Oeste do Paraná, Brasil. MÉTODOS: O estudo transversal foi realizado com amostra aleatória de 256 crianças. A coleta de dados (questionário, medidas antropométricas e amostras de sangue ocorreu de julho a setembro de 2007. A deficiência de ferro foi avaliada em termos de transferrina, hemoglobina, volume corpuscular médio, ferro sérico e eosinófilos. Na análise estatística dos dados foram obtidas as odds ratio bruta e ajustada (regressão logística, bem como os respectivos níveis de significância (p-valor. Para identificar diferenças significativas entre as medidas quantitativas, adotou-se a Análise de Variância e o teste de comparação múltipla de Tukey. RESULTADOS: A prevalência da anemia foi de 29,7%, sendo que 77,3% das amostras apresentaram baixa concentração de ferro. A antropometria não apontou deficiência de macronutrientes, porém mostrou obesidade acima dos índices médios. Os fatores associados à anemia e à deficiência de ferro foram: doenças frequentes na família (OR=10,02, condições de moradia (OR=5,05, tempo de creche (OR=3,05, número de moradores na residência (OR=2,83 e falta de saneamento (OR=2,20. CONCLUSÃO: A prevalência de anemia e a elevada deficiência de ferro detectada evidenciam um grave problema de saúde pública entre os pré-escolares do município de Cascavel, Paraná. Apesar da amplitude do problema, a anemia não está sendo reconhecida, prevenida e tratada adequadamente. Neste estudo são sugeridas algumas possíveis intervenções.OBJECTIVE: This study assessed the iron levels and prevalence of anemia and associated factors in children aged 6 to 24 months attending public daycare centers in Cascavel, Western Paraná, Brazil. METHODS: This cross-sectional study included 256 randomly sampled children

  16. QTL mapping for protein content in soybean cultivated in two tropical environments Mapeamento de QTL quanto ao conteúdo de proteína em soja cultivada em dois ambientes tropicais

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    Taís Cristina Bastos Soares

    2008-11-01

    Full Text Available The objectives of this study were to detect quantitative trait loci (QTL for protein content in soybean grown in two distinct tropical environments and to build a genetic map for protein content. One hundred eighteen soybean recombinant inbred lines (RIL, obtained from a cross between cultivars BARC 8 and Garimpo, were used. The RIL were cultivated in two distinct Brazilian tropical environments: Cascavel county, in Paraná, and Viçosa county, in Minas Gerais (24º57'S, 53º27'W and 20º45'S, 42º52'W, respectively. Sixty-six SSR primer pairs and 65 RAPD primers were polymorphic and segregated at a 1:1 proportion. Thirty poorly saturated linkage groups were obtained, with 90 markers and 41 nonlinked markers. For the lines cultivated in Cascavel, three QTL were mapped in C2, E and N linkage groups, which explained 14.37, 10.31 and 7.34% of the phenotypic variation of protein content, respectively. For the lines cultivated in Viçosa, two QTL were mapped in linkage groups G and #1, which explained 9.51 and 7.34% of the phenotypic variation of protein content. Based on the mean of the two environments, two QTL were identified: one in the linkage group E (9.90% and other in the group L (7.11%. In order for future studies to consistently detect QTL effects of different environments, genotypes with greater stability should be used.Os objetivos deste trabalho foram detectar QTL relativos ao conteúdo de proteína, em soja cultivada em dois ambientes tropicais divergentes, e construir um mapa genético para o conteúdo de proteína em genótipos adaptados a condições tropicais. Foram usadas 118 linhagens recombinantes endogâmicas de soja, obtidas do cruzamento entre as cultivares BARC 8 e Garimpo. A população de linhagens recombinantes endogâmicas foi cultivada em dois ambientes contrastantes: Cascavel, PR, e Viçosa, MG (24º57'S, 53º27'W; e 20º45'S, 42º52'W, respectivamente. Sessenta e seis pares de iniciadores SSR e 65 iniciadores RAPD

  17. Aspectos morfológicos de frutos e sementes e caracterização citogenética de Crotalaria lanceolata E. Mey. (Papilionoideae - Fabaceae Morphological aspects of fruits and seeds and cytogenetic characterization of Crotalaria lanceolata E. Mey. (Papilionoideae - Fabaceae

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    Débora Aparecida Verde de Andrade

    2008-09-01

    Full Text Available Frutos, sementes e plântulas de Crotalaria lanceolata, conhecida popularmente como guizo-de-cascavel, chocalho-de-cobra, xique-xique ou feijão-de-guizo, planta tóxica infestante que ocorre no Estado de São Paulo, foram estudadas morfologicamente e citogeneticamente. Os frutos são secos, deiscentes, polispérmicos e do tipo legume. As sementes são reniformes e o embrião é constituído de eixo embrionário e dois cotilédones. A testa pode apresentar variadas tonalidades de castanhos. A germinação é epígea e fanerocotiledonar. A espécie apresenta número cromossômico diplóide 2n = 16 com formulação cariotípica 12M + 4SM e comprimento cromossômico médio geral de 3,340 ± 0,689.Crotalaria lanceolata, known popularly as "guizo-de-cascavel", "xique-xique" or "feijão-de-guizo", is a toxic weed that occurs in the state of Sao Paulo. Fruits, seeds and seedlings were studied morphologically and cytogenetically. The fruits are dry, dehiscent, polyspermic legumes. The seeds are kidney shaped and the embryo consists of an embryonic axis and two cotyledons. The seed tegument comes in various chestnut tones. Germination is phanerocotylar and epigeal. The species has a mitotic chromosome number 2n = 16 with karyotype formula 12M + 4SM; overall mean chromosome length is 3.340 ± 0.689.

  18. Pentastomídeos de répteis do Brasil: revisão dos cephalobaenidae

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    A. Arandas Rêgo

    1983-12-01

    Full Text Available Foram estudados os Cephalobaenidae (Pentastomida, depositados na coleção helmintológica do Instituto Oswaldo Cruz e na coleção de parasitologia do Instituto Butantan. São redescritas e discutidas as espécies, Cephalobaena tetrapoda, C. freitasi, C. giglioli, Raillietiella furcocerca e Mahafaliella venteli. Esses parasitas foram coletados dos répteis: Lachesis sp., Drymarchon c. corais, Xenodon merremii, Crotatus terrificus, Amphisbaena sp., Tropidurus torquatus, Bothrops atrox, Mabuya punctata e de Bufo paracnemis (anfíbio.In this work the author studies Cephalobaenidae parasites using specimens from the helminthological collection of the Oswaldo Cruz and Butantan Institutes. This material was collected from Lachesis sp., Drymarchon corais, Xenodon merremii, Crotalus terrificus, Amphisbaena sp., Tropidurus torquatus, Bothrops atrox, Mabuya punctata (Reptilia and Bufo paracnemis (Amphibia. The species studied are Cephalobaena tetrapoda Heymons, 1922, Cephalobaena giglioli (Hett, 1924 comb. n., Cephalobaena freitasi (Motta & Gomes, 1968 comb. n., Raillietiella furcocerca (Diesing, 1836 and Mahafaliella venteli (Motta, 1965. C. recurvocauda becomes a synonym of C. tetrapoda and as do the specimens that Motta called erronously R. furcocerca. Raillietiella giglioli is changed to Cephalobaena giglioli (Hett, 1924 comb. n. The author describes here the male of C. giglioli for the first time. Travassostulida freitasi and T. acutiacanthus enter in synonymy with C. freitasi, and T. acutiacanthus is considered to be a subspecies of C. freitasi. Raillietiella gomesi becomes a synonym of R. furcocerca. The author discusses Mahafaliella venteli, and also questions the validity of the genus Gretillaria proposed by Motta for some species of Raillietiella. The latter is considered a synonym of Raillietiella.

  19. Physiological importance of the coronary arterial blood supply to the rattlesnake heart

    DEFF Research Database (Denmark)

    Hagensen, Mette; Abe, Augusto S.; Falk, Erling;

    2008-01-01

    supply to the outer compact layer in untreated snakes. Electrocardiogram (ECG), blood pressure (Psys) and heart rate (fH) were measured at rest and during enforced activity at day 1 and 4. Four days after occlusion of the coronary circulation, the snakes could still maintain a Psys and fH of 5.2±0.2 k......Pa and 58.2±2.2 beats min-1, respectively, during activity and the ECG was not affected. This was not different from sham-operated snakes. Thus, while the outer compact layer of the rattlesnake heart clearly has an extensive coronary supply, rattlesnakes sustain a high blood pressure and heart rate during...... remains unknown. In the present study we investigate the effects of permanent coronary artery occlusion in the South American rattlesnake (Crotalus durissus) on the ability to maintain heart rate and blood pressure at rest and during short term activity. We used colored silicone rubber (Microfil...

  20. Minimal volume regulation after shrinkage of red blood cells from five species of reptiles

    DEFF Research Database (Denmark)

    Kristensen, Karina; Berenbrink, Michael; Koldkjær, Pia

    2008-01-01

    Red blood cells (RBCs) from most vertebrates restore volume upon hypertonic shrinkage and the mechanisms underlying this regulatory volume increase (RVI) have been studied extensively in these cells. Despite the phylogenetically interesting position of reptiles, very little is known about their red...... cell function. The present study demonstrates that oxygenated RBCs in all major groups of reptiles exhibit no or a very reduced RVI upon ~ 25% calculated hyperosmotic shrinkage. Thus, RBCs from the snakes Crotalus durissus and Python regius, the turtle Trachemys scripta and the alligator Alligator...... was not characterized. It seems, therefore, that the RVI response based on NHE activation was lost among the early sauropsids that gave rise to modern reptiles and birds, while it was retained in mammals. An RVI response has then reappeared in birds, but based on activation of the NKCC. Alternatively, the absence...

  1. Immunogenicity of Bothrops atrox (Ophidia: Viperidae venom and its evaluation by immunoenzymatic methods Inmunogenicidad del veneno de Bothrops atrox (Ophidia: Viperidae y su evaluación por métodos inmunoenzimáticos

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    Gustavo A. Sandoval

    2012-02-01

    Full Text Available The immunogenicity of Bothrops atrox, “jergón”, venom was studied using ELISA and Western Blot methods, as well as cross-reactivity patterns against venoms of Bothrops brazili, Lachesis muta and Crotalus durissus. For this purpose, New Zealand white rabbits (2 kg aprox were immunized with four 500 μg doses of B. atrox venom in a period of 90 days. Antibody production was followed using ELISA technique, and title of hiper-immune serum was determined at the end of immunization protocol. Additionally, electrophoretic patterns of venoms were analyzed by SDS-PAGE and venom reactivity against obtained serum by ELISA and Western Blot. Immunization schedule allowed a pronounced antibody production since day 20 of protocol. At the end of process, serum title was 256000, which demonstrated both efficacy and usefulness of the developed procedure. On the other hand, studied venoms showed a heterogenic protein composition according to their electrophoretic patterns, whereas cross-reactivity values of 23,7%, 4,0% and 1,8% were obtained between B. atrox venom and B. brazili, L. muta and C. durissus venoms, respectively, using immunoenzymatic methods. According to our results, this procedure constitutes an initial step for further assays directed to optimization in immunoserum production for envenoming treatment and development of kits for diagnosis and species identification of snakes.Se estudió la inmunogenicidad del veneno de la serpiente Bothrops atrox, “jergón”, utilizando los métodos inmunoenzimáticos de ELISA y Western Blot, así como los patrones de reactividad cruzada empleando los venenos de las serpientes Bothrops brazili, Lachesis muta y Crotalus durissus. Para este fin se inmunizaron conejos albinos Nueva Zelanda (2 kg aprox con cuatro dosis de 500 μg del veneno de B. atrox en un periodo de 90 días. La producción de anticuerpos fue monitoreada mediante la técnica de ELISA, determinándose el título del suero hiperinmune obtenido

  2. Estabilidade e estudo de penetração cutânea in vitro da rutina veiculada em uma emulsão cosmética através de um modelo de biomembrana alternativo Stability and in vitro penetration study of rutin incorporated in a cosmetic emulsion through an alternative model biomembrane

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    André Rolim Baby

    2008-06-01

    Full Text Available A rutina é empregada como antioxidante e na prevenção da fragilidade capilar. Estudos de penetração in vitro através da pele humana seria a situação ideal, entretanto, há dificuldades de sua obtenção e manutenção de sua viabilidade. Entre os demais modelos de membrana, a muda de pele de cobra se apresenta como estrato córneo puro, fornecendo barreira similar ao humano e é obtida sem a morte do animal. Os objetivos desta pesquisa foram desenvolver e avaliar a estabilidade de uma emulsão cosmética contendo rutina e, como promotor de penetração cutânea, o propilenoglicol; e avaliar a penetração e a retenção cutânea in vitro da referida substância ativa da formulação, empregando um modelo de biomembrana alternativo. A emulsão foi desenvolvida com rutina e propilenoglicol, ambos a 5,0% p/p. Quantificou-se a rutina das emulsões por espectrofotometria a 361,0 nm, método previamente validado. A penetração e retenção cutânea in vitro foram realizadas em células de difusão vertical com muda de pele de cobra de Crotalus durissus, como modelo de biomembrana alternativo, e água destilada e álcool etílico absoluto 99,5% (1:1, como fluido receptor. O experimento foi conduzido em um período de seis horas, a 37,0 ± 0,5 ºC e agitação constante de 300 rpm. Empregou-se o método espectrofotométrico validado a 410,0 nm para a quantificação da rutina após penetração e retenção cutânea. A emulsão não promoveu a penetração cutânea da rutina através da muda de pele de C. durissus, retendo 0,931 ± 0,0391 mg de rutina/mg de muda de pele de cobra. Nas condições de armazenamento a 25,0 ± 2,0 ºC; 5,0 ± 0,5 ºC e 45,0 ± 0,5 ºC, a emulsão apresentou-se quimicamente estável durante 30 dias. De acordo com os resultados, a emulsão não favoreceu a penetração cutânea da rutina, mas apenas sua retenção no estrato córneo de C. durissus, condição considerada estável no período de 30 dias.Rutin is

  3. Estudo da morbidade em uma população idosa Estudio de la morbilidad en la población de ancianos Morbidity study in an elder population

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    Elizabeth Aparecida de Souza

    2011-06-01

    Full Text Available Este estudo objetivou identificar os agravos que mais acometeram os idosos no período de 1998 a 2005 e a capacidade funcional dos residentes na área de abrangência de quatro unidades básicas de saúde de diferentes regiões, no Município de Cascavel, Paraná, em 2007. Os dados de morbidade foram pesquisados no Sistema de Informações Hospitalar do Sistema Único de Saúde, e foram realizadas entrevistas com 141 idosos. No período estudado, a morbidade pouco variou: as doenças do aparelho circulatório foram responsáveis pelo maior número de internamentos. A média geral de doenças referidas pelos idosos foi de 2, com destaque para as cardiovasculares. Dentre os entrevistados, 136 (96,5% afirmaram realizar todas as atividades cotidianas, sem o auxílio de outras pessoas. O estudo mostra que é necessário implantar serviços voltados para as doenças crônicas, com foco na manutenção da máxima capacidade funcional pelo maior tempo possível, valorizando a autonomia e a autodeterminação dos idosos.El objetivo del estudio fue identificar las enfermedades que más afectaron a los ancianos en el período entre 1998 y 2005 y la capacidad funcional de los residentes en el área de cobertura de cuatro unidades básicas de salud de diferentes regiones en la cuidad de Cascavel, Paraná, en 2007. Los datos de morbilidad fueron investigados en el Sistema de Información Hospitalario del Sistema Único de Salud, e se realizaron encuestas con 141 ancianos. En el periodo estudiado, la morbilidad ha variado poco, las enfermedades del sistema circulatorio fueron responsables por el mayor número de internamientos. El promedio general de las enfermedades referidas por los mayores fue de 2, con relieve para las cardiovasculares. Entre los encuestados, 136 (o sea el 96,5% afirmaron realizar todas las actividades cotidianas sin la ayuda de los demás. El estudio muestra la necesidad de implementar servicios dirigidos a las enfermedades crónicas, con

  4. O tornar-se cuidadora na senescência Conviertendo cuidador en la vejez Becoming caregiver in the old age

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    Elizabeth Braz

    2009-06-01

    Full Text Available Este estudo, desenvolvido no município de Cascavel PR, com senescentes cuidadoras domiciliares de idosos dependentes, teve como objetivo verificar os motivos que as levaram a executarem esse papel. A metodologia utilizada foi a qualitativa, e a Representação Social foi eleita como caminho teórico. Foram critérios de inclusão: sexo feminino; idade superior ou igual a 60 anos; cuidadores domiciliares principais de idosos dependentes por um período igual ou superior a três meses. A coleta de dados deu-se entre novembro de 2006 a janeiro de 2007. A população constituiu-se de 13 senescentes, sendo 46,1% esposas, o que atribuímos a própria representação social construída. A representação social também está relacionada à figura da mulher, geralmente a mais velha da família, viúva ou solteira. Sem recursos e tratados com desigualdade pelo sistema, o idoso cuidador e, nesse caso, a idosa cuidadora senescente tornam-se mais um ponto vulnerável em nossa sociedade.Estudio, desarrollado en la ciudad de Cascavel-PR, con los cuidadoras ancianas domiciliarios de dependientes envejecidos, tenía como el objetivo, verificar las razones para ejecutar este papel. La metodología usada era cualitativa y como camino teórico la representación social. Los criterios de inclusión fueran: sexo femenino, edad igual o superior a los sesenta años, cuidadores principales domiciliarios de dependientes envejecidos, por un período igual o superior los tres meses. La recogida de datos fue hecha desde noviembre de 2006 hasta enero de 2007. La población consistió en 13 envejecido, siendo 46.1% esposas, qué atribuimos la representación social construida. La representación social también está relacionada, a la figura de la mujer, generalmente más vieja de la familia, viuda o soltera. Tratados sin recursos, el cuidador envejecido y en este caso, la cuidadora envejecida, se convierte en más un punto vulnerable en nuestra sociedad.The present study

  5. O tipo vivido de familiares de usuários de um centro de atenção psicossocial infantil El tipo vivido de familiares de usuarios de un centro de atención psicosocial infantil The experience lived by clients' family members of a child psychosocial care center

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    Gicelle Galvan Machineski

    2013-03-01

    Full Text Available O objetivo desta investigação foi compreender o tipo vivido de familiares de usuários de um Centro de Atenção Psicossocial Infantil. Trata-se de um estudo qualitativo, de abordagem fenomenológica, desenvolvido em um Centro de Atenção Psicossocial Infantil (CAPSi, localizado no município de Cascavel, Paraná. Os participantes da pesquisa foram onze familiares de usuários do referido serviço e que mais participavam dos cuidados com os usuários. Realizaram-se entrevistas semiestruturadas, e as informações foram analisadas por meio da metodologia da fenomenologia social. O tipo vivido dos familiares de usuários de um CAPSi mostrou-se como sendo: aquele que vivencia o comportamento do usuário; refere o encaminhamento de outros serviços; espera por tratamento; deseja a melhora do quadro clínico e vivencia o apoio do serviço aos familiares. Nesse sentido, o estudo pode contribuir para a reflexão sobre as práticas de Enfermagem em saúde mental aos usuários e familiares.El objetivo de esta investigación fue comprender el tipo vivido de familiares de usuarios de un Centro de Atención Psicosocial Infantil. Se trata de un estudio cualitativo de abordaje fenomenológico, desarrollado en un Centro de Atención Psicosocial Infantil (CAPSi, ubicado en el municipio de Cascavel, Paraná. Los participantes del estudio fueron once familiares de usuarios del referido servicio y que más participaban de los cuidados con los usuarios. Se realizaron entrevistas semiestructuradas y las informaciones se analizaron por medio de la metodología de la fenomenología social. El tipo vivido de los familiares de usuarios de un CAPSi se mostró como siendo: aquel que convive con el comportamiento del usuario; refiere el encaminamiento de otros servicios; espera por tratamiento; desea la mejora del cuadro clínico y convive con el apoyo de servicios a los familiares. En este sentido, el estudio puede contribuir para la reflexión sobre la práctica de la

  6. Criança hospitalizada: percepção das mães sobre o vínculo afetivo criança-família Niño hospitalizado: el vinculo afectivo niño-família Hospitalized children: mothers' perception about the affective child-family link

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    Beatriz Rosana Gonçalves de Oliveira

    1999-12-01

    Full Text Available Este trabalho objetivou verificar a percepção da mãe que tem seu filho doente hospitalizado, sobre a importância de sua permanência durante o período de internação para manutenção do vínculo afetivo criança-família. Os dados empíricos foram coletados com mães de crianças hospitalizadas, que não estavam acompanhando a internação do seu filho, num hospital público em Cascavel - Paraná, utilizando-se entrevista semi-estruturada. A análise dos dados demonstrou que as mães reconhecem a importância em permanecer com seu filho num período de estresse como o da hospitalização, porém, os fatores externos que influenciam seu cotidiano, muitas vezes não permitem que exerçam sua vontade de ficar com seus filhos.Este trabajo tiene como objetivo verificar la percepción de la madre que tiene su hijo enfermo hospitalizado, sobre la importancia de su permanencia durante el periodo de internación para la manutención del vinculo afectivo del niño-familia. Los dados empíricos fueron colectados con madres de niños hospitalizados, que no estaban acompañando la internación de su hijo, en un hospital publico en Cascavel ¾ Paraná, utilizase una entrevista semi-estructurada. El análisis de los datos demostró que las madres reconocen la importancia en permanecer con su hijo en un periodo de estrés como de la hospitalización, sin embargo, los factores externos que influencian su cotidiano, muchas veces no permiten que ejerzan su voluntad de quedar con sus hijos.This purpose of this research was to verify the perception of the mother who has her child sick and hospitalized, about the importance of her permanence during the hospitalization time for the maintenance of the affective child-family link. Empiric data were collected with mother's of hospitalized children, who were accompanying the hospitalization of their children in a public hospital at the Cascavel city in the West of Paraná, Brazil, through semi-structured interviews

  7. RÁDIO COMUNITÁRIA NA TRÍPLICE FRONTEIRA: participação étnica e de gênero na Rádio Norte FM

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    Maria Inês Amarante

    2016-08-01

    Full Text Available O artigo apresenta um recorte da pesquisa “Radiofonia Comunitária na Tríplice Fronteira: gênero, etnia e inclusão social midiática”, cuja proposta é um estudo aprofundado sobre a participação desses grupos nas rádios comunitárias das regiões das fronteiras entre o Paraná, o Paraguai e a Argentina e o modo como estas emissoras têm favorecido a cidadania comunicativa. Em destaque, o caso da Rádio Norte FM da cidade de Cascavel, na região oeste do Paraná. Atualmente, a emissora emite 24 horas e também pode ser ouvida online. Ela abre espaço para as manifestações culturais locais e procura dar vez e voz às reivindicações de diversos movimentos sociais, cedendo-lhes espaço ao longo da programação. Este trabalho foi realizado a partir de pesquisas bibliográfica e documental, visita de campo, entrevistas e questionários dirigidos a diretores e comunicadores.   PALAVRAS-CHAVE: Radiodifusão Comunitária; Gênero; Etnia; Participação. ABSTRACT This article presents part of the research “Community Radiophonic in the Triple Border: gender, ethnicity and social inclusion media”, whose proposal is a thorough study of this groups participation in community radio stations, in the regions of the borders, between the State of Parana, Paraguay and Argentina and how these vehicles have favored the communicative citizenship. Highlighting the case of Radio North FM, in the city of Cascavel, western region of Parana. Currently, the radio remains 24 hours on the air and is present online. It makes room for cultural events in all its expressions and it seeks to give time and voice to the demands of social movements giving them spaces along with the schedule of the programs. This work is based on bibliographic and documentary research, field visits, interviews and questionnaires addressed to directors and communicators.   KEY WORDS: Community Radios, gender, ethnicity and social participation.     RESUMEN El artículo presenta

  8. Determinação da curva característica de um painel fotovoltaico em condições reais de trabalho = Determination of the characteristic curve of a photovoltaic panel in real conditions of work

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    Estor Gnoatto

    2005-07-01

    Full Text Available No presente trabalho foi desenvolvido um estudo sobre um sistemafotovoltaico autônomo, com armazenamento de energia, instalado na Estação Experimental Agrometeorológica da Universidade Estadual do Oeste do Paraná, Estado do Paraná, Campus Cascavel, a uma latitude 24º 59’ Sul, longitude de 53º 26’ Oeste, com altitude de 682 metros, sob condições reais de campo, durante o ano de 2002. Foi determinada a curva característica do painel, utilizando uma aquisição de dados um "micrologger" da Campbell Scientific-INC modelo CR10X, programado para realizar uma leitura por segundo de cada canal e armazenar a média aritmética de cinco minutos dos dados das componentes detemperatura de operação do painel; tensão e corrente do sistema fotovoltaico. Baseando-se nesses dados pode-se determinar que a curva característica do painel em condições reais de campo apresenta uma perda de 24% em seu rendimento em relação ao padrão STC (condições padrão de teste.The purpose of the present work was to develop the study of anautonomous photovoltaic system, with storage of energy, installed in the Agrometeorological Experimental Station of Universidade Estadual do Oeste do Paraná, State of Paraná, Campus Cascavel, at 24º 59’ latitude south, 53º26 longitude west, 682 meters of altitude, under real field conditions, during the year 2002. Efficacy of the photovoltaic panels has been evaluated under real field conditions, as well as to determine its characteristic linear fit. A 'micrologger' Campbell Scientific-INC model CR10X was used to acquire data, and it was programmed to accomplish a reading per second in each channel and to store the arithmetic average of data from five minutes of operational temperature of the panel, photovoltaic system tension and current. According to results, it could determined that the panel characteristic linear fit in real field conditions shows 24% less field than standard STC (Standard Test Conditions.

  9. Influência do espaçamento e população de plantas sobre doenças de final de ciclo e oídio e caracteres agronômicos em soja = Influence of row spacing and plant population on late season disease severity, powdery mildew and agronomic characters in soybean

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    Jorge Luiz Knebel

    2006-07-01

    Full Text Available Este trabalho foi conduzido em Cascavel, Estado do Paraná, na safra de2004/2005, utilizando a cultivar de soja CD 202, para avaliar a influência do espaçamento e da população de plantas sobre a severidade de (DFC doenças de final de ciclo (Septoria glycines e Cercospora kikuchii e oídio da soja (Microsphaera diffusa e caracteres agronômicos da cultura. Os tratamentos foram 3 espaçamentos (22,5; 45,0 e 67,5 cm e 3 populações (200; 400; e 600 mil plantas ha-1. As DFC tiveram menor severidade no espaçamento reduzido e na população mais baixa, enquanto a ocorrência de oídio não se alterou. Em todos os espa��amentos, quanto maior a população de plantas, maior a altura final e, conseqüentemente, maior o acamamento de plantas. O número de legumes/planta foi superior no maior espaçamento e reduziu com o aumento da população; o número de grãos/planta diminuiu com o aumento da população; e a produtividade foi superior no menor espaçamento.This study was carried out in Cascavel, Paraná State, during the growing season 2004/2005, using soybean cultivar CD 202. The aim was to evaluate the influence of row-spacing and plant population on lateseason disease severity (Septoria glycines and Cercospora kikuchii, soybean powdery mildew (Microsphaera diffusa and agronomic characters of the culture. The treatments were three row spacing (22.5; 45.0 and 67.5 cm and three plant populations (200; 400; and 600 thousand plants ha-1. Late season disease had smaller severity in the reduced spacing and in the lowest population, while the powdery mildew occurrence did not modify with these factors. In all spacing, as larger the plants density, larger the plants height and consequently larger the plants fallen. The number pods per plant was higher with the largest spacing and it decreased with the population increase; the number of grains per plant decreased with the population increase; and the productivity was higher in the smallest spacing.

  10. Análise de um sistema de aquecimento de água para residências rurais, utilizando energia solar A water heating system analysis for rural residences, using solar energy

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    Luiz H. Basso

    2010-02-01

    Full Text Available A conscientização da importância do meio ambiente tem incentivado o estudo de novas fontes energéticas renováveis e menos poluentes. Dentre essas fontes, a energia solar destaca-se por ser perene e limpa. A utilização da energia solar em sistemas de aquecimento de água residencial rural pode colaborar com a economia de energia elétrica, base da matriz energética brasileira. Conhecer os fatores que influenciam na operação de um sistema de aquecimento de água por energia solar é importante na determinação de sua viabilidade técnica, visando a sua difusão em residências rurais. Para tanto, construiu-se um protótipo, no câmpus da Faculdade Assis Gurgacz, em Cascavel - PR, com características similares a um equipamento utilizado em residências para dois habitantes, para funcionar com circulação natural ou termossifão e sem auxílio de sistema de aquecimento complementar. O equipamento mostrou-se viável tecnicamente, alcançando a temperatura mínima para banho de 35 °C, sempre que a radiação solar foi superior a 3.500 Wh m-2, o que aconteceu para a maioria dos dias estudados.The awareness of the importance of the environment has stimulated the study of new renewed energy sources and less pollutant. Amongst these sources, solar energy stands alone for being perennial and clean. The use of solar energy in systems of agricultural residential water heating, can complement the economy of electric energy, base of the Brazilian energy matrix. Knowing the factors that influence the operation of a system of water heating by solar energy is important in determining their technical viabilities targeting their distribution in agricultural residences. To evaluate equipment of water heating for solar energy, a prototype was constructed in the campus of Assis Gurgacz College, in Cascavel,State of Paraná, Brazil, with similar characteristics to equipment used in residences for two inhabitants, to function with natural circulation or

  11. Distribuição de frequência e temporal de chuvas intensas =

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    Luciana Espíndula de Quadros

    2011-07-01

    Full Text Available A distribuição generalizada de valores extremos (GEV tem tido grandeaceitação para discrição dos eventos máximos naturais. Estudos sobre o assunto mostram que a distribuição GEV ajusta valores mais próximos à realidade quando há necessidade de extrapolação dos dados amostrais para grandes períodos de retornos e com o intuito de contribuir para o planejamento de atividades que são influenciadas pela intensidade de água precipitada foi ajustado um modelo de distribuição de probabilidade de chuva intensa por meio da GEV, utilizando momentos LH para estimar seus parâmetros e o teste estatístico proposto por Wang (1998 para verificação da qualidade dos ajustes desenvolvidos noambiente Matlab. Analisaram-se séries históricas de precipitação máximas para diferentes durações obtidas de pluviográfos no município de Cascavel, Estado do Paraná. Além disso, as referidas séries foram ajustadas pela distribuição Gumbel para efeito de comparação coma GEV. Este trabalho mostra que a distribuição Gumbel subestima a distribuição GEV para grandes períodos de retorno.The generalized extreme value distribution (GEV has had great acceptance for discretion of the maximum natural events. Previous studies show that GEV distribution fits values closer to realitywhen there is need for extrapolation of sampling data for longer periods of returns. In order to contribute to the planning of activities that are influenced by the intensity of precipitated water, we adjusted a model of probability distribution of heavy rain through GEV, using LH moments for estimating its parameters and statistical test proposed by Wang (1998 for checking the quality of the adjustments developed in the Matlab. We analyzed historicaltime series of maximum rainfall for different durations obtained from rain gauges in the city of Cascavel, Paraná State. Moreover, these series were fitted by Gumbel distribution for purposes of comparison with the GEV

  12. Plano de amostragem para Dichopelmus notus (Eriophyidae na cultura da erva-mate Sampling plan for Dichopelmus notus (Eriophyidae on mate-tea orchard

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    Guilherme Bertoldo

    2008-06-01

    Full Text Available O objetivo deste trabalho foi avaliar a amostragem presença-ausência para Dichopelmus notus Keifer (Eriophyidae na cultura da erva-mate. O trabalho foi realizado em plantação comercial, em Cascavel, PR, entre abril de 1999 e julho de 2000. Procederam-se a coletas quinzenais de 240 folhas em diferentes partes de 10 plantas. A contagem dos ácaros nas folhas foi realizada, em laboratório, com auxílio de microscópio estereoscópico. Foi determinado o padrão de agregação com o modelo da potência de Taylor, e também a proporção de folhas infestadas e o número de amostras requeridas foram estimados por análise de regressão. D. notus apresentou distribuição agregada. A proporção de folhas infestadas estimadas pelo modelo matemático mostrou ser um parâmetro confiável para se estimar a densidade populacional do ácaro, sendo que o número de amostras requeridas foi pequeno, o que viabiliza a aplicação prática do método.The objective of this research was to evaluate presence-absence sampling plan for Dichopelmus notus Keifer (Eriophyidae on mate-tea orchard. This study was conducted in Cascavel, State of Paraná, Brazil, from April 1999 to July 2000, in a mate-tea tree commercial plantation. Biweekly sampling of 240 leaves in different parts of 10 plants was conducted, and the number of mites was registered. The aggregation pattern was determined using the Taylor’s power law model. The proportion of the infested leaves and the number of required samples were estimated by regression analysis. D. notus presented aggregated distribution. The estimated proportion of infested leaves showed to be reliable in order to estimate the population density of mites. The number of sample unities required to estimate infestation density was low, which makes practical application of this sampling method to be feasible.

  13. Alelopatia de cultivos de cobertura vegetal sobre plantas infestantes = Allelopathy of cover crop on weed plants

    Directory of Open Access Journals (Sweden)

    Luciene Kazue Tokura

    2006-07-01

    Full Text Available O presente trabalho avaliou o potencial alelopático de cultivos de cobertura vegetal de trigo, aveia preta, milheto, nabo forrageiro e colza sobre o desenvolvimento de plantas infestantes e verificou qual das coberturas vegetais exerce maior controle sobre as mesmas. Os cultivos de cobertura vegetal foram implantados sob preparo convencional (uma aração e uma gradagem no Núcleo Experimental de Engenharia Agrícola (NEEA, da Universidade Estadual do Oeste do Paraná (Unioeste, Cascavel, Estado do Paraná. Mensalmente, realizou-se o acompanhamento e identificação das plantas infestantes emersas nas áreas de cobertura vegetal no período de agosto de 2000 a agosto de 2001. Os resultados obtidos permitiram concluir que das espécies encontradas, o capim marmelada foi o que apresentou maior potencial alelopático e a erva-de-santa-maria o menor. As coberturasvegetais que apresentaram melhor controle do total de plantas infestantes presentes na área experimental, incluindo àquelas com reconhecido potencial alelopático, foram aveia preta, colza, nabo forrageiro e milheto.This work evaluated the cover crop allelopathic potential of wheat, black oat, pearl millet, turnip and rape on the development of weed plants. It also verified which cover crop has larger control on the weed plants. The cover crop was implanted under conventional tillage (one disk plowing plus one disk harrowing in the Experimental Nucleus of Agricultural Engineering (NEEA, of the State University of the West of Paraná (Unioeste, Cascavel, Paraná State. Monthly (from August 2000 to August 2001, weed plants identification in the cover crop area was made. Results showed that from the found species, the alexander grass was the one that presented larger allelopathic potential, and, the mexican-tea was the one that presented smaller control. The vegetable coverings that presented larger control of the total of weed plants in the experimental area, including those with

  14. Profile of users of drugs for the treatment of chronic hepatitis B available through the Brazilian Public Health System

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    Astrid Wiens

    Full Text Available Treatment for chronic hepatitis B in Brazil are funded by the Ministry of Health and by the state Departments of Health. Clinical protocol and therapeutic guidelines approve the use of adefovir, entecavir, interferon-a, lamivudine, and tenofovir for the treatment of chronic hepatitis B. The aim of this study was to establish the profile of users of these drugs in the state of Paraná. A cross-sectional study was conducted with patients under treatment in Paraná in August 2011. The following data were obtained: gender, hepatitis B used drug, International Classification of Diseases, and regional health unit. The monthly cost of these drugs for the public health system was also calculated. 1,093 patients registered were found, 70% male, and 2.6% co-infected with the delta agent. Tenofovir was the drug most commonly used (355 users. The highest prevalence was found in the regional health units of Pato Branco, Cascavel, Foz do Iguaçú, Francisco Beltrão, Toledo, Londrina, and Maringá. The annual cost for the public health system in Paraná was U$1,066,867. Through this study it was possible to investigate the distribution and profile of users of drugs for the treatment of chronic hepatitis B in Paraná in August 2011.

  15. Comportamento sexual de risco entre estudantes universitárias dos cursos de ciências da saúde Risky sexual behavior among university students in health science courses

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    Angela Maria Moser

    2007-04-01

    Full Text Available OBJETIVO: Avaliar o comportamento sexual de estudantes do sexo feminino do Setor de Ciências da Saúde da Universidade Federal do Paraná e do Setor de Ciências Biológicas e da Saúde da Universidade Estadual do Oeste do Paraná, Campus Cascavel. MÉTODOS: Participaram todas as estudantes matriculadas nos referidos setores, no mês de junho de 2001, com idade entre 18 e 24 anos completos, sendo que a amostra final foi constituída por 572 estudantes em Curitiba e 395 em Cascavel. Foram avaliados a idade, relacionamento familiar, religiosidade, participação em aulas de educação sexual, idade de início de atividade sexual, uso de métodos anticoncepcionais e preservativos, e número de parceiros para avaliar a conduta sexual. Sexo seguro foi definido pelo uso de preservativo em todas ou na maioria das relações sexuais pelas estudantes monogâmicas, incluindo as estudantes poligâmicas com uso de preservativo em todas as relações sexuais. Sexo inseguro foi definido pelo uso ocasional de preservativo pelas estudantes monogâmicas, e pelo não uso sistemático entre as estudantes poligâmicas. O instrumento de coleta de dados foi um questionário auto-respondido. A análise dos dados foi feita no SPSS, utilizando o teste do Qui-quadrado de Pearson e Yates, teste do p de Wilcoxon-Gehan, análise bivariada e regressão logística. RESULTADOS: Não houve diferença significativa na conduta sexual de risco entre as estudantes e a cidade de estudo. Cerca de 50% das estudantes de 18 a 20 anos e 70% das de 21 a 24 anos tinham vida sexual ativa. A abstinência esteve associada a menor idade, maior freqüência a culto religioso e ao bom relacionamento das estudantes com os pais. A prática de sexo seguro esteve diretamente associada a menor idade e a morar fora da residência da família. Ter participado de aulas de educação sexual esteve associada a maior abstinência, mas não a sexo seguro, e a relação não se manteve na an

  16. A water heating system analysis for rural residences, using solar energy; Analise de um sistema de aquecimento de agua para residencias rurais, utilizando energia solar

    Energy Technology Data Exchange (ETDEWEB)

    Basso, Luiz H.; Souza, Samuel N.M. de; Siqueira, Jair A.C.; Nogueira, Carlos E.C.; Santos, Reginaldo F. [Universidade Estadual do Oeste do Parana (UNIOESTE), Cascavel, PR (Brazil). Programa de Pos-Graduacao em Engenharia Agricola], emails: melegsouza@yahoo.com, ssouza@unioeste.br, jairsiqueira@unioeste.br, cecn1@yahoo.com.br, rfsantos@unioeste.br

    2010-01-15

    The awareness of the importance of the environment has stimulated the study of new renewed energy sources and less pollutant. Amongst these sources, solar energy stands alone for being perennial and clean. The use of solar energy in systems of agricultural residential water heating, can complement the economy of electric energy, base of the Brazilian energy matrix. Knowing the factors that influence the operation of a system of water heating by solar energy is important in determining their technical viabilities targeting their distribution in agricultural residences. To evaluate equipment of water heating for solar energy, a prototype was constructed in the campus of Assis Gurgacz College, in Cascavel,State of Parana, Brazil, with similar characteristics to equipment used in residences for two inhabitants, to function with natural circulation or thermo siphon and without help of a complementary heating system. The equipment revealed technical viability, reaching the minimum temperature for shower, of 35 deg C, whenever the solar radiation was above the 3,500 Wh m{sup -2}, for the majority of the studied days. (author)

  17. Aerobic fitness and biological and sociodemographic indicators in female school children

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    Roberto Régis Ribeiro

    2013-05-01

    Full Text Available Aerobic fitness is an important health-related fitness component. The objective of this study was to analyze the influence of age, growth, body composition, sexual maturation and socioeconomic status on the aerobic fitness of female school children attending public schools in Cascavel, Paraná, Brazil. Across-sectional epidemiological study was conducted in 2006 on 1,317 school children aged 8 to17 years. Body mass, height and triceps and medial calf skinfold thickness were measured and relative body fat (%BF was calculated. Sexual maturation was self-assessed using a breast development scale (M1 to M5. The questionnaire proposed by the Brazilian Association of Research Companies was used to assess socioeconomic status. Aerobic fitness was estimated by the 20-m shuttle runtest. The results were analyzed using two-way ANOVA, Tukey post hoc test, Spearman and Pearson correlation coefficients, and multiple linear regression with 95% confidence intervals. Three variables (age, %BF and socioeconomic status showed an interaction (p<0.05 in the prediction of VO2peakrel (R2=0.73. All independent variables (body mass, height, age, %BF, socioeconomic status, and sexual maturation were significant predictors (p<0.05 of VO2picoabs (R2=0.88. In conclusion, chronological age and sexual maturation were the best predictors of VO2peakabs (positive and VO2peakrel (negative.

  18. Scientific controversies on biological knowledge construction: investigating a continued formation course for teachers with respect for human biological evolution

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    Marcelo Erdmann Bulla

    2016-08-01

    Full Text Available The research here presented has as central theme the human biological evolution, its scientific controversies and the continued formation of science and biology teachers. We evaluate the development of a teaching sequence on the topic, emphasizing the scientific controversy regarding the supposed fossil hominid Ardipithecus ramidus (“Ardi” in a continued formation course for teachers of science and biology of basic public network Cascavel-PR and region. The empirical work involved collecting data from the responses provided by teachers to an initial questionnaire and a final. The analysis and data discussion has highlighted the importance of scientific controversy for the development of scientific knowledge and the urgency to insert the contents of human evolution in subjects on the initial formation of courses in licentiate of Biological Sciences. It is necessary also to offer continued formation courses to include such content for teachers already inserted in schools. We conclude that teaching biology and science using scientific controversies may be in satisfactory teaching tool to introduce the history and nature of science, since scientific activity is permeated by conflicts.

  19. EXPERIÊNCIAS, SEMELHANÇAS E ALGUMAS PARTICULARIDADES NA ORGANIZAÇÃO DO TRABALHO NA CATAÇÃO DE MATERIAIS RECICLÁVEIS NAS COOPERATIVAS DE RECICLAGEM: COAAFI E COOTACAR

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    Solange Queiroz Ribeiro

    2013-12-01

    Full Text Available O artigo tem como foco principal entender o trabalho coletivo de dois grupos de trabalhadores catadores organizados em cooperativas de reciclagem, mais propriamente, visando apreender a organização do trabalho como enfrentamento as questões conflitantes do capital, assim como, a luta para além da organização interna e a trajetória dos trabalhadores na catação dos resíduos sólidos, compostos por materiais recicláveis, que sobrevivem a partir do que é considerado lixo para alguns e meio de sobrevivência para outros, nos municípios de Cascavel e Foz do Iguaçu, região Oeste do Paraná. O estudo revela sua importância no sentido de entender a dinâmica geográfica do trabalho na catação dos resíduos, pois a Geografia enquanto ciência humana permite entender suas relações sociais, assim como é o caso destes trabalhadores. Palavras chave: Organização do trabalho; trabalho; catação de recicláveis; Geografia. 

  20. Calorific value for compositions with biodiesel of fat chicken and diesel oil; Valor calorifico para composicoes com biodiesel da gordura de frango e oleo diesel

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Marcelo Jose da [Universidade de Campinas (FEAGRI/UNICAMP), SP (Brazil). Fac. de Engenharia Agricola], email: marcelo.jose@feagri.unicamp.br; Souza, Samuel N.M. de; Souza, Abel A. de; Martins, Gislaine I. [Universidade Estadual do Oeste do Parana (CCET/UNIOESTE), Cascavel, PR (Brazil). Centro de Ciencias Exatas e Tecnologicas], emails: ssouza@unioeste.br, abel.sza@hotmail.com, iastiaque@yahoo.com.br

    2011-07-01

    The biodiesel fuel is a renewable source of alternative fuel used in diesel cycle engines. The production of biodiesel involves the reaction of methanol with fatty acids of animal or vegetable. The production of biodiesel from chicken fat can be very attractive for some regions from Brazil with high poultry production, as in the Parana West and Santa Catarina West. In this study , the goal was the lower calorific value of the compositions between biodiesel and diesel oil: 100% Diesel oil (B0), 20% biodiesel (B20), 40% biodiesel (B40), 60% biodiesel (B60), 80% biodiesel (B80 ), 100% biodiesel (B100). The biodiesel used was acquired in the Centre for Development and Diffusion of technologies on the Assis Gurgacz College, in Cascavel city. The nominal production capacity of the unit is 900 liters on period of 8 hours. The model of the calorimeter used, was the E2K. The lower calorific value of B100 composition was 35.388 MJ kg-1 and the diesel oil was 41.299 MJ kg-1. With the measuring of the caloric value of six samples mix of diesel oil and biodiesel, was obtained a linear function decrease of the calorific value when increased it the proportion of biodiesel from chicken fat into fuel. (author)

  1. NURSING PROCESS, ACCORDING TO THE CONCEPTUAL MODEL OF WANDA HORTA, APPLIED TO A FAMILY MET BY THE REGIONAL CORE OF HOME CARE, DISTRITO FEDERAL.

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    Prince Vangeris Silva Fernandes de Lima

    2014-05-01

    Full Text Available The case report describes the nursing care, systematized according to the conceptual model of Wanda Horta, who was dismissed to a family accompanied by a core team of Regional home care, an administrative region of the Federal District through prioritization, based on the scale of Cascavel, a member of this for such a public service. The context part according to reports from the Discipline community health nursing in ' 02 and ' Stage, mandatory mode sold to students from the undergraduate program in nursing, University of Brasilia. At this juncture, described the history and nursing diagnoses, a welfare plan, as well as the prescription, the evolution and prognosis of nursing. In the residence there was the importance of specific health care provided not only by the nurse, but for the entire multidisciplinary team, customers with chronic clinical pictures and aggravated, but stable, that demand care that exceed those offered by primary health care. Seeking the completeness of the care, professionals and students involved started for the opportunity to visualize and better understand the patterns of relationships established between the client, family and healthcare professionals, with an emphasis on how the actors involved in this scenario mobilized in support of ailing client.

  2. Mudanças no trabalho e na vida de bancários portadores de Lesões por Esforços Repetitivos: LER

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    Murofuse Neide Tiemi

    2001-01-01

    Full Text Available A LER constitui-se num dos graves problemas de saúde dos trabalhadores na atualidade. Com o objetivo de analisar as principais mudanças no trabalho e na vida dos portadores de LER de membros de uma Associação de Portadores de LER de Cascavel-PR, foi desenvolvido o estudo, de natureza qualitativa. Foram realizadas entrevistas semi-estruturadas, com nove bancários. Os primeiros sintomas surgiram no período coincidente com a automação e a introdução de novas tecnologias no sistema financeiro brasileiro e as mudanças na organização do trabalho. Aumento do volume de trabalho e da digitação, horas-extra, ausências de pausas, monotonia, repetitividade, ritmo intenso e controle da produtividade e tensão constituíram os principais elementos. O agravamento das lesões afetou a capacidade produtiva e implicou na dependência para execução de atividades no trabalho e fora dele. O desafio está em garantir os direitos desses trabalhadores.

  3. Allelopathy of Camelina sativa Boiss. (Brassicaceae on germination and early development of Bidens pilosa (L. and Glycine max (L. Merr

    Directory of Open Access Journals (Sweden)

    Jéssica da Silva

    2011-12-01

    Full Text Available This study evaluated the possible presence of allelopathic potential in camelina, as well as its effect on germination and early growth of soybean seedlings and beggarticks, in order, to verify the possibility of cultivating it with soybeans in a culture rotation system and its use as a herbicide. The experiments were carried out at the Laboratory of Plant Physiology of Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil. The design was completely randomized and the evaluations were held daily. The parameters under analysis were: germination percentage, time and average speed of germination, and average root lenght. The data obtained underwent the F-Test and the mean values were compared through Tukey’s test, at a 5% probability level. The results confi rm the presence of allelopathic potential in camelina. It was found that this species can be considered an option for cultivating with soybean, due to the positive allelophatic interference caused in the culture and because it can be used in the control of weeds such as beggartick, having in mind that that it has delayed the development of the seedlings tested.

  4. Acesso ao cuidado à saúde da criança em serviços de atenção primária

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    Rosane Meire Munhak da Silva

    2014-10-01

    Full Text Available Realizou-se uma pesquisa de abordagem qualitativa com base no referencial metodológico da hermenêutica dialética, com o objetivo de identificar o atributo acesso da atenção primária para a resolução dos problemas de saúde de crianças menores de um ano a partir dos relatos de pais e cuidadores. Envolveram-se 16 cuidadores de crianças atendidas em unidades de pronto atendimento de Cascavel-PR, em 2010. Foram reconhecidas quatro categorias temáticas: Aconselhamento familiar ao buscar atenção à saúde da criança; Ausência de acolhimento ao primeiro contato; Presença de classificação de risco para atenção à saúde da criança; Barreiras que impedem o acesso à atenção à saúde. Concluiu-se que as famílias exibiram dificuldades para alcançar resolutividade aos problemas de saúde dos filhos, mediante a falta de acesso aos serviços de atenção primária.

  5. O (DESINTERESSE DOS PROFESSORES FRENTE ÀS ATIVIDADES EM CONTRATURNO

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    Juliana Alves Da Silva Ubinski

    2015-01-01

    Full Text Available La educación a tiempo completo ha sido un tema tratado en la investigación educativa y en la elaboración de políticas educativas brasileñas. Algunos cambios en estas políticas han posibilitado innovaciones con respecto a una mayor permanencia del alumnado en el centro educativo, atendiendo algunas demandas sociales al respecto. La motivación de los profesores responsables de las actividades extra curriculares es esencial para el desarrollo efectivo de los estudiantes. Este trabajo tiene como objetivo presentar las percepciones de los profesores con respecto a las actividades curriculares complementadas con un horario extraclase. La investigación se llevó a cabo con profesores de una escuela pública de la ciudad de Cascavel, Paraná, Brasil, mediante la aplicación de un cuestionario. Los resultados obtenidos demuestran la necesidad de buscar un mayor compromiso de los profesores en relación con las actividades extracurriculares en las escuelas donde trabajan, para que así se sientan motivados a fomentar esta práctica.

  6. Evaluation of the principle of coordination in primary health care of the child in Londrina-Pr

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    Gisleine Tíemi Souza

    2015-11-01

    Full Text Available Primary Care is a set of universally accessible services that promotes and protects health and prevents and treats diseases, and it is considered the initial access to the Health System. Four main essential attributes are present: accessibility, continuity, integrality and coordination. The coordination is the network of several health related services and actions that must be synchronized and continuous regardless of the location in which they occur. The goal of this study was to evaluate this coordination principle in Primary Care provided to children in 39 UBS (tr. From Basic Health Unit in the urban area of the city of Londrina. The research is multicentric (Londrina, Cascavel and João Pessoa, descriptive and quantitative – with use of PCATool-Brasil for children. In this instrument, coordination is subdivided in integration of care (which refers to the relation between Basic Health Care and specialties and the information system (which evaluates the health data and file availability. Amongst the 609 subjects submitted to interview, only 29.2% reported that a specialist examined the child. The coordination-integration of care score was 7.393 and the coordination-information system score was 7.620. From the eight questions concerning the coordination attribute that can be numeric-evaluated, three had scores below 6.6. The score should be higher than that to meet the concept of Primary Care. The conclusion is that the coordination attribute had a high score despite the three questions with a low score.

  7. Determinação da curva característica de um painel fotovoltaico em condições reais de trabalho - DOI: 10.4025/actascitechnol.v27i2.1484

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    Estor Gnoatto

    2005-03-01

    Full Text Available No presente trabalho foi desenvolvido um estudo sobre um sistema fotovoltaico autônomo, com armazenamento de energia, instalado na Estação Experimental Agrometeorológica da Universidade Estadual do Oeste do Paraná, Estado do Paraná, Campus Cascavel, a uma latitude 24º 59’ Sul, longitude de 53º 26’ Oeste, com altitude de 682 metros, sob condições reais de campo, durante o ano de 2002. Foi determinada a curva característica do painel, utilizando uma aquisição de dados um "micrologger" da Campbell Scientific-INC modelo CR10X, programado para realizar uma leitura por segundo de cada canal e armazenar a média aritmética de cinco minutos dos dados das componentes de temperatura de operação do painel; tensão e corrente do sistema fotovoltaico. Baseando-se nesses dados pode-se determinar que a curva característica do painel em condições reais de campo apresenta uma perda de 24% em seu rendimento em relação ao padrão STC (condições padrão de teste

  8. Effects of a labor gym program in mental workload of workers from rectory of UNIOESTE.

    Science.gov (United States)

    Galvão, Isabele Maia; Vilagra, José Mohamud; Moreira, Helenara Salvati Bertolossi; Moro, Antônio Renato Pereira; Cruz, Roberto Moraes; Reis, Pedro Ferreira; Moreira, Mauricio Bertolossi

    2012-01-01

    The present work is a quality-quantitative study of cause-effect type. The objective of this study was to investigate the effect of a Labor Gym (LG) program in mental workload of workers from rectory of the Universidade Estadual do Oeste do Paraná (Unioeste) Cascavel. The sample consisted of 35 employees. For data collection, it was used the NASA-TLX questionnaire to evaluate the mental workload of employees. Then, the participants performed sessions of LG, totaling 20 classes. After this period the employess were re-evaluated. To characterize the sample, data were tabulated and a descriptive analysis was performed using the SPSS 15.0. The interpretation of the NASA-TLX questionnaire was based on its own methodology. Before the LG sessions, the total average dimensions analyzed was 79 (sd: 11,05), representing a high rate of mental workload. After the period of LG, the total average of final mental workload decreased, but it remained with a high rate (x:75, sd: 13.42). In relation to the comparative analysis of the initial and final averages of mental workload, by t test (bipolar), there was no significant difference (p = 0.071668).

  9. Temporomandibular joint sounds and disc dislocations incidence after orotracheal intubation.

    Science.gov (United States)

    Rodrigues, Estela T; Suazo, Iván C; Guimarães, Antonio S

    2009-01-01

    The aim of this study was to analyze the temporomandibular joint (TMJ) disc displacement and articular sounds incidence after orotracheal intubation. A prospective cohort study was conducted in the Hospital Universitário do Oeste do Paraná (HUOP), in Cascavel, Brazil. 100 patients (aged 14-74 years, mean 44 years), 34 male and 66 female, in need of surgical procedure with orotracheal intubation were evaluated. The anterior disc displacement with reduction incidence and the nonclassifiable sounds incidence by the Research Diagnostic Criteria Axis I was evaluated in all patients after orotracheal intubation. The patients was evaluated one day before and until two days after the procedure. Eight percent present with anterior disc displacement with reduction and 10% presented nonclassifiable sounds after the orotracheal intubation. There was no correlation of any kind regarding gender related influence in the incidence of disc dislocations (P = 0.2591) and TMJ sounds (P = 0.487). Although anterior disc dislocations and TMJ sounds after anesthetic with orotracheal intubation presented a low incidence (8%-10%), it is recommended that the evaluation of TMJ signs and symptoms be done before the anesthetic procedure to take care with susceptible patients manipulation.

  10. Uso de álcool entre adolescentes escolares: um estudo-piloto

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    Luana Dallo

    2011-12-01

    Full Text Available O uso de álcool entre adolescentes é um tema que preocupa profissionais da saúde pelos prejuízos acarretados e pela displicência da sociedade quanto ao uso desta droga por essa faixa etária. Este artigo analisa o consumo de álcool entre alunos do ensino médio de três escolas de Cascavel-PR. O instrumento utilizado foi o Alcohol Use Disordens Identification Test (AUDIT, além de levantamento de informações sobre o nível socioeconômico, religião e o beber problemático de familiar. O resultado mostrou que 34,7% dos estudantes bebem de maneira arriscada, não havendo diferenças significativas por escola, gênero e nível socioeconômico. Ter familiar que bebe foi considerado fator de risco e ter religião, fator protetor. Esta pesquisa pode alertar a sociedade paranaense para a necessidade de estudos mais amplos, com o objetivo de desenvolver políticas públicas que incluam projetos de prevenção e intervenção dirigidos à população jovem.

  11. Mudanças no trabalho e na vida de bancários portadores de Lesões por Esforços Repetitivos: LER

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    Neide Tiemi Murofuse

    Full Text Available A LER constitui-se num dos graves problemas de saúde dos trabalhadores na atualidade. Com o objetivo de analisar as principais mudanças no trabalho e na vida dos portadores de LER de membros de uma Associação de Portadores de LER de Cascavel-PR, foi desenvolvido o estudo, de natureza qualitativa. Foram realizadas entrevistas semi-estruturadas, com nove bancários. Os primeiros sintomas surgiram no período coincidente com a automação e a introdução de novas tecnologias no sistema financeiro brasileiro e as mudanças na organização do trabalho. Aumento do volume de trabalho e da digitação, horas-extra, ausências de pausas, monotonia, repetitividade, ritmo intenso e controle da produtividade e tensão constituíram os principais elementos. O agravamento das lesões afetou a capacidade produtiva e implicou na dependência para execução de atividades no trabalho e fora dele. O desafio está em garantir os direitos desses trabalhadores.

  12. EXISTENCIAL DE SUAS VIVÊNCIAS

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    Elionésia Marta dos Santos

    2011-01-01

    Full Text Available El estudio tuvo como objetivo comprender los sentimientos de las personas que sufren dolor por la muerte de un ser querido con cáncer. Se trata de una investigación de carácter cualitativo, a la luz de la fenomenología existencial de Martin Heidegger, realizada entre abril y junio de 2009. Los seis participantes entrevistados se seleccionaron de los archivos del Programa de Asistencia y Cuidado en el Hogar, en la ciudad de Cascavel-PR. A los familiares se les hizo la siguiente pregunta: "¿Cómo se está sintiendo por la muerte de su ser querido?" Del análisis surgieron tres temáticas existenciales: Angustia ante la posibilidad de una muerte inminente, el descubrimiento de la propia finitud, y, experimentar la temporalidad de estar en duelo. Se concluye que el enfermero debe caminar al lado de la persona que está de luto, ya que él necesita sentirse apoyado en todo el proceso, pues vivir el duelo significa transitar un camino para sobrevivir a la ausencia de un ser querido y encontrar un nuevo sentido para la existencia

  13. Methemoglobinemia associated with loxoscelism Meta-hemoglobinemia associada ao loxoscelismo

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    Orlando C. de O. Barreto

    1990-02-01

    Full Text Available In twenty five patients who presented the cutaneous form of loxoscelism, serum haptoglobin and lactic dehydrogenase, erythrocyte glucose-6-phosphate dehydrogenase, glutathione reductase, glutathione peroxidase, methemoglobin, bilirubin and reticulocytes were investigated after bite. No hemolysis was detected but an increase in methemoglobin was found in 54% of the cases; in 7% it was between 1.1% and 2%, in 27% it ranged from 2.1% to 4%, and in 20% from 4.1% to 8%. Blood samples of a normal, blood group 0 individual and of a patient who exhibited methemoglobinemia after Loxosceles bite were incubated separately with antisera against Loxosceles gaucho, Crotalus terrificus, Bothrops jararaca, with Loxosceles gaucho venom and 0.3% phenol. No methemoglobin was found after 1, 4,8 and 15 days in both sets of samples. At the 25th day all the samples, including the controls, exhibited similar methemoglobin reductase decrease. The data suggest that the methemoglobinemia which occurs in 50% of the patients probably arises from in vivo venom metabolism, inasmuch as the crude venom does not induce methemoglobinemia.Vinte e cinco pacientes que apresentaram a forma cutânea do loxoscelismo foram estudados após a picada, determinando-se a glicose-6-fosfato desidrogenase, glutationa redutase e glutationa peroxidase eritrocitárias, haptoglobina e latico desidrogenase séricas, bilirrubina, reticulócitos e meta-hemoglobina. Não foi observada hemólise aumentada, mas foi detectado aumento da meta-hemoglobina em 54% dos casos: em 7% entre 1,1% e 2%, em 27% variou de 2,1% a 4%, e em 20% de 4,1 a 8%. Amostras de sangue de um indivíduo normal do grupo 0 de uma paciente que exibiu meta-hemoglobina após picada por Loxosceles foram incubadas separadamente com anti-soros contra Loxosceles gancho, Crotalus terrificus e Bothrops jararaca, com veneno de Loxosceles gaucho e fenol a 3%, e não se detectou aumento de meta-hemoglobina depois de 1, 4, 8 e 15 dias em todas

  14. A STUDY OF THE COMPETITION OF LECITHIN AND ANTITOXIN FOR CL. WELCHII LECITHINASE

    Science.gov (United States)

    Zamecnik, Paul C.; Lipmann, Fritz

    1947-01-01

    Lecithin has been found to interfere with the combining reaction of Cl. welchii alpha toxin (lecithinase) and its antitoxin. If the lecithinase is first brought into contact with lecithin, and the antitoxin is then added, the antitoxin fails to stop the enzymatic reaction, but gradually decelerates it. If the lecithinase is brought into contact with both lecithin and antitoxin at the same instant, it appears to combine in part with each, and the enzymatic process takes place at a reduced rate, which gradually declines further. If the lecithinase is first brought into contact with antitoxin, before the lecithin is added, the enzymatic reaction is completely inhibited. This ability of lecithin to inhibit the antitoxin-toxin combination cannot be explained adequately as a non-specific coating of the toxin-enzyme by the lecithin. It is rather suggested that lecithin and antitoxin compete specifically for combination with the same regions on the enzyme molecule. Lecithin has similarly been found to interfere with the combination of Crotalus terrificus venom and its antiserum. The above findings provide a partial explanation for the lack of effectiveness of antitoxin when given late in the course of Cl. welchii infection. PMID:19871624

  15. Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-Like/Gyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom

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    Frank Denis Torres-Huaco

    2013-01-01

    Full Text Available We report a rapid purification method using one-step chromatography of SVSP Rhombeobin (LMR-47 from Lachesis muta rhombeata venom and its procoagulant activities and effects on platelet aggregation. The venom was fractionated by a single chromatographic step in RP-HPLC on a C8 Discovery BIO Wide Pore, showing high degree of molecular homogeneity with molecular mass of 47035.49 Da. Rhombeobin showed amidolytic activity upon BAρNA, with a broad optimum pH (7–10 and was stable in solution up to 60°C. The amidolytic activity was inhibited by serine proteinase inhibitors and reducing agents, but not chelating agents. Rhombeobin showed high coagulant activity on mice plasma and bovine fibrinogen. The deduced amino acid sequence of Rhombeobin showed homology with other SVSPs, especially with LM-TL (L. m. muta and Gyroxin (C. d. terrificus. Rhombeobin acts, in vitro, as a strong procoagulant enzyme on mice citrated plasma, shortening the APTT and PT tests in adose-dependent manner. The protein showed, “ex vivo”, a strong defibrinogenating effect with 1 µg/animal. Lower doses activated the intrinsic and extrinsic coagulation pathways and impaired the platelet aggregation induced by ADP. Thus, this is the first report of a venom component that produces a venom-induced consumptive coagulopathy (VICC.

  16. Aspectos citoquímicos e morfológicos de elementos sanguíneos das serpentes dos gêneros Bothrops e Crotalus mantidas em cativeiro no serpentário do Instituto Vital Brasil

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    L.M. Kindlovits

    Full Text Available RESUMO A criação de serpentes peçonhentas em cativeiro vem se tornando prática cada vez mais difundida no país. Dessa forma, o conhecimento do manejo e da clínica de serpentes se torna prioritário, a fim de permitir maior sobrevida dos animais. No que concerne a serpentes peçonhentas, dados hematológicos já foram descritos na literatura, no entanto, apesar dos recursos utilizados, os dados analisados ainda são insipientes. Com o objetivo de caracterizar, morfologicamente, as células sanguíneas e de esclarecer diferenças morfológicas e funcionais, foram coletadas amostras de sangue de 50 serpentes pertencentes ao plantel do Instituto Vital Brasil. Foram confeccionados e analisados por microscopia óptica e citoquimicamente os esfregaços sanguíneos corados por métodos de Romanowsky e citoquímicos. Foi possível diferenciar as células, caracterizar e confirmar a existência de eosinófilo em B. atrox e C. durissus. Concluiu-se que a caracterização celular pode fornecer evidências indispensáveis ao entendimento da fisiologia de serpentes.

  17. Parasitological and immunological diagnoses from feces of captive-bred snakes at Vital Brazil Institute

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    Janaína Lima de Souza

    Full Text Available Fecal samples from 56 snakes at the Vital Brazil Institute, in the city of Niterói, Rio de Janeiro, were tested using the sedimentation and flotation techniques to investigate the evolutionary forms of parasites such as helminths and protozoa, and using enzyme immunoassay techniques to detect antigens of Cryptosporidium sp. and Giardiasp. Among the animals tested, 80.3% were positive for parasites. Out of these, there were 16 Bothrops jararaca, 16 B. jararacussu and 13 Crotalus durissus. The prevalence of parasitic nematodes was 41.1%, and nematodes were found in all three snake species. Among these, the most frequent finding was eggs of Kalicephalus sp., which were diagnosed in 25% of the snakes. The positivity for protozoa detected using parasite concentration techniques was 75%, including oocysts of Caryospora sp. in 75%, cysts with morphology similar to Giardia sp. 3.6%, amoeboid cysts in 41.1% and unsporulated coccidia oocysts in 8.9%. Immunoassays for Cryptosporidium sp. antigens produced positive findings in 60.7%. Pseudoparasites were detected in 64.3%. These results show that there is a need to improve the sanitary handling of captive-bred snakes, and also for the animal house that supplies rodents to feed them. The results also highlight that diagnostic tests should be performed periodically on stool specimens from captive-bred snakes.

  18. [The efficacy of the bothropic-crotalic antivenom in the neutralization of the main Bothrops jararacussu venom effects].

    Science.gov (United States)

    dos-Santos, M C; Gonçalves, L R; Fortes-Dias, C L; Cury, Y; Gutiérrez, J M; Furtado, M de F

    1992-01-01

    Myonecrosis is one of the effects of Bothrops jararacussu venom, from which a myotoxin was isolated showing structural homology to phospholipase A2 (PLA2), but without enzymatic activity. Such myotoxic activity is also present in the Crotalus durissus terrifucus venom, and is attributed to crotoxin and to PLA2 (crotoxin B), the basic component of the crotoxin complex. The Bothrops jararacussu venom showed three proteins with immunologic identity to PLA2 from crotoxin. The bothropic (AB) and the bothropic/crotalic (AB/C) anti-venoms, two commercial polyvalent anti-venoms produced at Instituto Butantan, were compared in order to assess their capacity for neutralization of the lethal, hemorrhagic, coagulant and myotoxic activities of Bothrops jararacussu venom. Both anti-venoms showed the same level of hemorrhagic activity neutralization. However, AB/C was about three times more efficient than AB in neutralizing the myotoxic activity, and two times more potent for neutralization of lethality and coagulant activity of Bothrops jararacussu venom. These data suggest that the use of AB/C could be of value in the treatment of patients bitten by snakes of this species.

  19. Substituted thiobenzoic acid S-benzyl esters as potential inhibitors of a snake venom phospholipase A2: Synthesis, spectroscopic and computational studies

    Science.gov (United States)

    Henao Castañeda, I. C.; Pereañez, J. A.; Jios, J. L.

    2012-11-01

    4-Chlorothiobenzoic acid S-benzyl ester (I), 3-nitrothiobenzoic acid S-benzyl ester (II), 4-nitrothiobenzoic acid S-benzyl ester (III) and 4-methylthiobenzoic acid S-benzyl ester (IV) were prepared and characterized by 1H and 13C NMR, Mass spectrometry and IR spectroscopy. Quantum chemical calculations were performed with Gaussian 09 to calculate the geometric parameters and vibrational spectra. Phospholipase A2 (PLA2) was purified from Crotalus durissus cumanensis venom by molecular exclusion chromatography, followed by reverse phase-high performance liquid chromatography. Two studies of the inhibition of phospholipase A2 activity were performed using phosphatidilcholine and 4-nitro-3-octanoyloxybenzoic acid as substrates, in both cases compound II showed the best inhibitory ability, with 74.89% and 69.91% of inhibition, respectively. Average percentage of inhibition was 52.49%. Molecular docking was carried out with Autodock Vina using as ligands the minimized structures of compounds (I-IV) and as protein PLA2 (PDB code 2QOG). The results suggest that compounds I-IV could interact with His48 at the active site of PLA2. In addition, all compounds showed Van der Waals interactions with residues from hydrophobic channel of the enzyme. This interaction would impede normal catalysis cycle of the PLA2.

  20. Parasitological and immunological diagnoses from feces of captive-bred snakes at Vital Brazil Institute.

    Science.gov (United States)

    Souza, Janaína Lima de; Barbosa, Alynne da Silva; Vazon, Adriana Prado; Uchôa, Claudia Maria Antunes; Nunes, Beatriz Coronato; Cortez, Myrian Bandeira Vianna; Silva, Valmir Laurentino da; Más, Leonora Brazil; Melgarejo, Aníbal Rafael; Bastos, Otilio Machado Pereira

    2014-01-01

    Fecal samples from 56 snakes at the Vital Brazil Institute, in the city of Niterói, Rio de Janeiro, were tested using the sedimentation and flotation techniques to investigate the evolutionary forms of parasites such as helminths and protozoa, and using enzyme immunoassay techniques to detect antigens of Cryptosporidium sp. and Giardia sp. Among the animals tested, 80.3% were positive for parasites. Out of these, there were 16 Bothrops jararaca, 16 B. jararacussu and 13 Crotalus durissus. The prevalence of parasitic nematodes was 41.1%, and nematodes were found in all three snake species. Among these, the most frequent finding was eggs of Kalicephalus sp., which were diagnosed in 25% of the snakes. The positivity for protozoa detected using parasite concentration techniques was 75%, including oocysts of Caryospora sp. in 75%, cysts with morphology similar to Giardia sp. 3.6%, amoeboid cysts in 41.1% and unsporulated coccidia oocysts in 8.9%. Immunoassays for Cryptosporidium sp. antigens produced positive findings in 60.7%. Pseudoparasites were detected in 64.3%. These results show that there is a need to improve the sanitary handling of captive-bred snakes, and also for the animal house that supplies rodents to feed them. The results also highlight that diagnostic tests should be performed periodically on stool specimens from captive-bred snakes.

  1. Evaluación comparativa de dos métodos para determinar la actividad de fosfolipasa A en venenos de serpientes

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    Fanny Lazo

    2014-06-01

    Full Text Available Se ha evaluado la actividad de fosfolipasa A en seis venenos de serpientes comparando las ventajas de un método espectrofotométrico, previamente adaptado a nuestras condiciones de laboratorio, frente al método macroscópico de retardo en la coagulación de una emulsión lipoproteica. En ambos casos, la actividad de fosfolipasa A estuvo presente en los venenos de Micrurus spixii, Crotalus durissus, Bothrops brazili, Lachesis muta y Bothrops atrox y la actividad decreció en orden. En cambio, la actividad de la enzima en el veneno de Micrurus surinamensis sólo fue detectada por el método espectrofotométrico. Así mismo, los resultados basados en la cantidad de veneno utilizado para medir la actividad, mostraron una mayor sensibilidad con el método espectrofotométrico en comparación con el método macroscópico ya que se requirieron cantidades menores en el orden de 2 a 10 veces.

  2. Anti-platelet effect of cumanastatin 1, a disintegrin isolated from venom of South American Crotalus rattlesnake.

    Science.gov (United States)

    Da Silva, Manuel; Lucena, Sara; Aguilar, Irma; Rodríguez-Acosta, Alexis; Salazar, Ana M; Sánchez, Elda E; Girón, Maria E; Carvajal, Zoila; Arocha-Piñango, Carmen L; Guerrero, Belsy

    2009-03-01

    Disintegrins have been previously described in the venom of several snake families inhibiting signal transduction, cell-cell interactions, and cell-matrix interactions and may have therapeutic potential in heart attacks, thrombotic diseases, and cancers. This investigation describes the first disintegrin isolated from South American Crotalus venom (Venezuelan rattlesnake Crotalus durissus cumanensis), which inhibits platelet adhesion to matrix proteins. C. d. cumanensis crude venom was first separated on a Sephadex G-100 column into 4 fractions (SI to SIV). Crude venom and SIII fraction significantly diminished platelet adhesion to fibrinogen (Fg) and to fibronectin (Fn). Anti-adhesive SIII fraction was further separated by DEAE-Sephacel followed by C-18 reverse phase high performance liquid chromatography (HPLC). The platelet anti-adhesive fraction obtained was designated as cumanastatin-1. This disintegrin has a mass of 7.442 kDa as determined by mass spectrometry (MALDI-TOF/TOF) and pI of 8.5. Cumanastatin-1 also inhibited ADP-induced platelet aggregation with an IC(50) of 158 nM. However, it did not significantly inhibit collagen and thrombin-induced platelet aggregation. Cumanastatin-1 considerably inhibited anti-alpha(IIb)beta(3) integrin binding to platelets in a dose-dependent manner; however, it did not present any effect on the alpha(5)beta(1) integrin or on P-selectin.

  3. Oral microbiota of Brazilian captive snakes

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    MG Fonseca

    2009-01-01

    Full Text Available The present work aimed to determine the oral microbiotic composition of snakes from São José do Rio Preto city, São Paulo State, Brazil. Ten snake species, comprising the families Boidae, Colubridae, Elapidae and Viperidae, were submitted to microbiological examination of their oral cavity, which indicated positivity for all buccal samples. Gram-negative bacilli, gram-negative cocci bacilli, gram-positive bacilli and gram-positive cocci were isolated from the snakes. Among isolated bacterium species, the occurrence of coagulase-negative staphylococci in the buccal cavity of Crotalus durissus (Viperiade, Eunectes murinus (Boidae, Mastigodryas bifossatus (Colubridae and Bacillus subtilis, common to oral cavity of Bothrops alternatus (Viperidae and Phalotris mertensi (Colubridae, was detected. It was observed higher diversity of isolated bacteria from the oral cavity of Micrurus frontalis (Elapidae and Philodryas nattereri (Colubridae, as well as the prevalence of gram-positive baccillus and gram-positive cocci. The composition of the oral microbiota of the studied snakes, with or without inoculating fangs, is diverse and also related to the formation of abscesses at the bite site in the victims of the ophidian accidents, and to pathogenic processes in the snakes that host these microorganisms.

  4. Ocorrência de Commelina villosa como planta daninha em áreas agrícolas no estado do Paraná-PR, Brasil Occurence of Commelina villosa as weed in Paraná State, Brazil

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    Dalva C. Rocha

    2000-01-01

    Full Text Available As trapoerabas pertencem à família Commelinaceae e são plantas daninhas de difícil controle em diferentes regiões do país. No Brasil, a espécie Commelina benghalensis destaca-se como a principal trapoeraba infestante nas culturas de soja, milho, café e citros. Outras duas espécies desse gênero, Commelina diffusa e Commelina erecta, também são conhecidas como infestantes de ocorrência freqüente no território nacional. Commelina villosa está registrada, até o momento, apenas para os Estados da Bahia e Goiás além do Distrito Federal. O presente trabalho teve como objetivos caracterizar e registrar a ocorrência de C. villosa no Estado do Paraná, onde pode estar sendo confundida com outras trapoerabas, principalmente C. benghalensis. Exemplares de C. villosa e C. benghalensis foram coletados, de forma aleatória, em lavouras de soja, feijão e milho, nos municípios de Ponta Grossa, Tibagi, Piraí do Sul, Guarapuava, Pato Branco, Francisco Beltrão, Cascavel, Campo Mourão e Londrina. Parte desse material foi herborizado para a confecção de exsicatas e outra parte foi mantida in vivo, cultivada no Departamento de Botânica, do Instituto de Biociências de Botucatu-UNESP. Caracteres morfológicos descritivos e quantitativos foram avaliados e as espécies comparadas entre si. C. villosa distinguiu-se de C. benghalensis por apresentar folhas maiores (9,76 x 3,26 cm, elíptica a elípticaestreita, sésseis, de coloração verde escura com manchas violáceas na face inferior, filetes translúcidos, entre outras características. A ocorrência de C. villosa no Paraná foi constatada em todos os municípios amostrados, com exceção de Campo Mourão e Londrina.Commelinaceae are weeds with unsatisfactory control in different parts of the country. Commelina benghalensis is the main Commelinaceae weed in soybean, corn, coffee and orange crops in Brazil. Commelina diffusa and Commelina erecta are also known as common weeds in national

  5. Unidades de manejo a partir de dados de produtividade = Determination of management zones using yield data.

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    Lairton Milani

    2006-10-01

    Full Text Available A agricultura de precisão é um conjunto de tecnologias que visa o aumento da eficiência com base no manejo diferenciado de áreas agrícolas. Neste contexto, é importante estabelecer metodologias nos quais informações de produtividade, solo ou indicadores compostos possam ser utilizados para a determinação de unidades de manejo. O objetivo deste trabalho foi desenvolver uma metodologia para definição de unidades de manejo em função de mapas de produtividade de cinco safras agrícolas. Foi medida a produtividade da cultura soja, durante os anos de 1998 a 2002, de uma área localizada no município de Cascavel, Estado do Paraná, e utilizando técnicas de geoestatística foram gerados mapas de produtividade. A área amostrada possui 1,74 ha, sendo constituída de 256 parcelas, 128 com manejo químico localizado e 128 sem manejo químico localizado. Na colheita, utilizou-se uma colhedora de parcelas e verificou-se dependência espacial para os sistemas de cultivo, em todos os anos. Os valores pontuais de produtividade de soja de cada ano foram padronizadosutilizando-se a técnica denominada escore padrão, em seguida reclassificados em baixo, médio e alto, o que possibilitou a comparação das produtividades de diferentes anos e a geração de um mapa de produtividade média. Com a produtividade padronizada e ocoeficiente de variação classificados em baixo, médio e alto foi gerado um mapa com as unidades de manejo. A metodologia mostrou-se eficiente para identificar regiões homogêneas.Precision agriculture is a set of technologies that aims the efficiency increase based on the differentiated management of agricultural areas. In this context, it is important to establish methodologies to use the yield information, soil or indicators in the determination of management zones. The aim of this paper was to develop a methodology for the definition of management zones according to yield maps of five growing seasons. The soybean yield was

  6. Avaliação genética em erva-mate pelo procedimento BLUP individual multivariado sob interação genótipo x ambiente Genetic evaluation in Ilex paraguariensis by the multivariate individual BLUP procedure with genotype-environment interaction

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    Rosangela Maria Simeão

    2002-11-01

    Full Text Available Os objetivos deste trabalho foram estimar parâmetros genéticos e fenotípicos e realizar a predição de valores genéticos de matrizes e procedências de erva-mate (Ilex paraguariensis. Foram avaliadas 164 progênies de oito procedências, em três locais (Ivaí, PR, Guarapuava, PR e Rio Azul, PR, em relação ao caráter produção de massa foliar (PMF. Os componentes de variância, parâmetros genéticos e valores genéticos foram estimados pelo procedimento REML/BLUP individual (máxima verossimilhança restrita/melhor predição linear não viciada, realizando a análise multivariada para os três locais. Os coeficientes de herdabilidade individual, no sentido restrito, para o caráter PMF foram 0,15 em Ivaí, 0,62 em Guarapuava e 0,23 em Rio Azul. A baixa magnitude desses coeficientes em Ivaí e Rio Azul demanda a utilização de métodos de seleção que utilizem todos os efeitos aleatórios do modelo estatístico. O efeito de procedências foi significativo em Ivaí e Rio Azul, com correlação fenotípica intraclasse de 0,13 em Ivaí e de 0,09 em Rio Azul. As procedências apresentaram correlação genética de 0,95 entre os locais Ivaí e Rio Azul. Nesses locais, as procedências foram mais estáveis nos diferentes ambientes do que as progênies. Foram preditos os valores genéticos em relação a todas as procedências e matrizes em todos os locais quanto ao caráter avaliado. As melhores procedências são Barão de Cotegipe, Quedas do Iguaçu, Cascavel e Ivaí.The objectives of this paper were to estimate genetic and phenotypic parameters and to predict breeding values for parents and provenances of Ilex paraguariensis. Eight provenances with 164 progenies were evaluated on three sites (Ivaí, PR, Guarapuava, PR and Rio Azul, PR for the trait leaf weight (LW. All the variance components, parameters and breeding values were obtained by REML/BLUP (restricted maximum likelihood/best linear unbiased prediction procedure at the

  7. Efeito do armazenamento à baixa temperatura (-4 ºC na cor e no teor de acidez da farinha de trigo Effect of storage at low temperature (-4 ºC in the color and acidity content of wheat flour

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    Fernanda Ortolan

    2010-03-01

    Full Text Available Durante o armazenamento de farinha de trigo, podem ocorrer mudanças bioquímicas que resultam em alterações nutricionais e tecnológicas, sendo que em baixa temperatura estas mudanças ocorrem mais lentamente. Entre as alterações, pode-se citar o aumento da acidez, a redução do pH e as modificações na sua cor. O presente trabalho teve por objetivo avaliar os efeitos do armazenamento à baixa temperatura (-4 ºC na cor e na acidez da farinha de trigo durante seis meses e correlacionar os resultados obtidos para investigar se a acidez tem relação com a cor da farinha. Foram utilizadas farinhas provenientes de dez genótipos de trigo cultivados em Cascavel, no Estado do Paraná. A cor foi determinada no sistema CIEL*a*b* através dos parâmetros de cor: L* (luminosidade, a* e b* (coordenadas de cromaticidade. Usou-se procedimento da AOAC para determinação da acidez de lipídios. As farinhas apresentaram tendência ao branqueamento durante o período de seis meses de armazenamento, com ocorrência de leve aumento da acidez, mas dentro dos teores aceitáveis. A acidez não foi considerada um fator envolvido na alteração da cor das farinhas no armazenamento à baixa temperatura, pois não houve correlação entre estes dois parâmetros.During wheat flour storage, biochemical changes can occur which lead to nutritional and technological alterations, but in low temperature these changes can occur more slowly. Among the reactions, we may note are an increase in acidity, reduction of pH and modifications to its color. The present work aimed to evaluate the effects of storage at low temperature (-4 ºC on the color and acidity of wheat flour over six months and to correlate the results obtained to discover whether the acidity has any relationship with the color of the flour. Flours of ten wheat genotypes, cultivated in Cascavel, in Paraná State were used. The color was determined via the CIEL*a*b* system using the color parameters: L

  8. Propriedades físicas de Latossolo Vermelho distroférrico típico sob sistemas de manejo na sucessão soja-milho no período de três anos = Physical properties in distrofic Red Latosol under management systems in the succession soybean-maize in three years

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    Reny Adilmar Prestes Lopes

    2007-12-01

    Full Text Available Este trabalho avaliou alterações das propriedades físicas teor de água, densidade e porosidade do solo em áreas sob sistemas de semeadura direta e preparo convencional nas culturas de soja e milho em três anos agrícolas. O experimento foi instalado no NúcleoExperimental de Engenharia Agrícola da Unioeste (Cascavel, Estado do Paraná. Nos dois primeiros anos foi implantada a cultura de soja; no terceiro ano, milho, como cultura de verão, e aveia preta e nabo forrageiro, como cobertura de inverno. Durante os três anos,foram observados redução do teor de água e densidade do solo e aumento da porosidade. As variações nas propriedades físicas do solo mostraram relação diretamente proporcional entre teor de água e densidade do solo e inversamente para porosidade. O solo apresentoumelhorias nas condições físicas pelo acréscimo de porosidade e redução de densidade com as culturas de aveia preta+nabo forrageiro e milho. O manejo soja/milho em rotação com aveia preta+nabo forrageiro mostrou-se mais adequado na melhoria das condições físicas dosolo do que os sistemas de manejo, uma vez que nenhum dos sistemas teve maior destaque na melhoria das propriedades físicas avaliadas em todo o período.This study evaluated alterations in the physical properties water content, soil density and porosity in areas under no tillage and tillage systems in the cultures of soybean and maize in three agricultural years. The experiment was carried out at the Experimental Nucleus of Agricultural Engineering of Unioeste (Cascavel, state of Paraná. Soybean culture occurred in the first two years and in the third year maize, as summer crop, black oats and forage turnip as winter cover crops. During these three years the study observed reduction of water content and soil density andincrease of porosity . The variations as regards the physical properties of the soil showed direct proportional relation between water content and soil density and was

  9. Quantitative genetic analysis of methylxanthines and phenolic compounds in mate progenies Análise genética quantitativa de metilxantinas e compostos fenólicos em progênies de erva-mate

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    Euclides Lara Cardozo Junior

    2010-02-01

    Full Text Available The objective of this work was to determine the contents of methylxanthines, caffeine and theobromine, and phenolic compounds, chlorogenic and caffeic acids, in 51 mate progenies (half-sib families and estimate the heritability of genetic parameters. Mate progenies were from five Brazilian municipalities: Pinhão, Ivaí, Barão de Cotegipe, Quedas do Iguaçu, and Cascavel. The progenies were grown in the Ivaí locality. The contents of the compounds were obtained by high performance liquid chromatography (HPLC. The estimation of genetic parameters by the restricted maximum likelihood (REML and the prediction of genotypic values via best linear unbiased prediction (BLUP were obtained by the Selegen - REML/BLUP software. Caffeine (0.248-1.663% and theobromine (0.106-0.807% contents were significantly different (p0.5. The two different progeny groups determined for chlorogenic (1.365-2.281% and caffeic (0.027-0.037% acid contents were not significantly different (pO objetivo deste trabalho foi determinar o teor de metilxantinas, cafeína e teobromina, e de compostos fenólicos, ácido clorogênico e ácido cafeico, em 51 progênies de erva-mate e estimar componentes de variância e herdabilidade. As progênies de erva-mate eram oriundas de cinco municipios brasileiros: Pinhão, Ivaí, Barão do Cotegipe, Quedas do Iguaçu e Cascavel. Essas progênies foram cultivadas na localidade de Ivaí. O conteúdo dos compostos foi obtido por cromatografia líquida de alta eficiência (CLAE. Na estimativa dos componentes da variância e dos parâmetros genotípicos, utilizou-se a metodologia de modelos mistos para a obtenção da melhor predição linear não viciada (BLUP dos efeitos genotípicos e o processo da máxima verossimilhança restrita (REML, processados pelo programa Selegen - REML/BLUP. Os conteúdos de cafeína (0,248-1,663 % e teobromina (0,106-0.807% foram significativamente (p0,5. Foram determinados dois diferentes grupos de progênie para

  10. Teor de nitrato em alface hidropônica em função de vazões e períodos de pós-colheita Nitrate content in hydroponic lettuce in function of flow rate and post-harvest periods

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    Antonio Aprígio

    2012-09-01

    Full Text Available O objetivo do trabalho foi determinar o teor de nitrato (NO3- presente em plantas de alface (Lactuca sativa L. da cultivar Vera, conduzida por hidroponia, em função de vazões e períodos de pós-colheita. O experimento foi realizado no município de Cascavel, PR, em casa de vegetação da Universidade Estadual do Oeste do Paraná (UNIOESTE. As plantas foram nutridas mediante utilização de uma solução nutritiva caracterizada por possuir condutividade elétrica média de 1,2 dS m-1 e que, ao percorrer toda a bancada de cultivo, retornava ao tanque de irrigação e reiniciava o processo, constituindo, desta forma, um sistema fechado. No experimento, utilizou-se o delineamento inteiramente casualizado com oito repetições que foram representadas, individualmente, por duas plantas de alface, no esquema fatorial (3 x 4. Os tratamentos resultaram da combinação entre três vazões (0,5; 1,0 e 1,5 L min-1 e quatro períodos de armazenamento pós-colheita (0; 24; 48 e 72 h. A determinação do teor de nitrato foi realizada por meio do método colorimétrico e os valores obtidos foram inferiores ao limite máximo exigido pela Comunidade Europeia. Os fatores investigados influenciaram significativamente os teores médios de nitrato.The objective of this study was to determine the nitrate (NO3- content present in lettuce plants (Lactuca sativa L. of the Vera cultivar, produced under hydroponic system, in function of different flow rates and post-harvest periods. The experiment was conducted in the city of Cascavel-PR, in a greenhouse of the Universidade Estadual do Oeste do Paraná (UNIOESTE. The plants were nourished by the use of a nutrient solution, characterized by mean electrical conductivity of 1.2 dS m-1 which after passing the entire growing bench, returned to the irrigation tank and restarted the process, forming a closed system. The experiment was conducted in completely randomized design with eight replications, which were represented

  11. Suscetibilidade de Oligonychus yothersi (Acari: Tetranychidae ao fungo Beauveria bassiana Susceptibility of Oligonychus yothersi (Acari: Tetranychidae to the fungus Beauveria bassiana

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    Renato Cassol de Oliveira

    2002-03-01

    Full Text Available A cultura da erva-mate (Ilex paraguariensis é uma importante atividade econômica no Sul do Brasil. Com o aumento de consumo nos anos oitenta, cresceram também as áreas de monocultura, gerando condições favoráveis para o aumento populacional de insetos e ácaros fitófagos, entre eles o ácaro vermelho Oligonychus yothersi. Este estudo avaliou a suscetibilidade do ácaro vermelho O. yothersi a vários isolados do fungo Beauveria bassiana. O experimento foi conduzido em Cascavel, PR. Discos foliares de erva-mate (2,2 cm² foram previamente infestados com 20 fêmeas adultas. Alíquotas de 1 mL de cada suspensão de conídios dos diferentes isolados de B. bassiana, ajustadas na concentração de 1,0x10(8 conídios mL-1 foram pulverizadas, separadamente, sobre um conjunto de 5 discos. Os discos foliares foram mantidos flutuando em água destilada, em caixas plásticas (3 cm de diâmetro, sob condições controladas. Diariamente, os ácaros mortos foram transferidos para câmara úmida, para confirmação de mortalidade causada pelo fungo. A mortalidade total variou entre 77 e 98% (6 dias após a aplicação, não permitindo diferenciar os isolados quanto à virulência (P>0,05. Já a mortalidade confirmada variou entre 19 e 75%, permitindo diferenciá-los (PThe Paraguay tea (Ilex paraguariensis crop is an important economical activity in Southern Brazil. With the increase of tea consumption in the 80s, the number of monoculture crops also increased, creating favorable conditions for the development of phytophagous insect and mite populations, such as the red mite Oligonychus yothersi. The objective of this study was to evaluate the susceptibility of the red mite O. yothersi to the several strains of the Beauveria bassiana fungus. The experiments were carried out in Cascavel, PR, Brazil. Leaf disks of Paraguay tea (2.2 cm² were previously infested with 20 adult females. Five disks were inoculated with 1 mL of each conidial suspension of B

  12. A eficiência de um programa de reabilitação audiológica em idosos com presbiacusia e seus familiares The efficiency of an auditory rehabilitation program in elderly people with presbycusis and their family

    Directory of Open Access Journals (Sweden)

    Christine Vieira Ruschel

    2007-06-01

    Full Text Available OBJETIVO: O objetivo dessa pesquisa foi desenvolver um programa de treinamento de estratégias de comunicação para indivíduos idosos, portadores de deficiência auditiva, usuários de aparelhos de amplificação sonora individual e seus familiares. Além disso, objetivamos verificar a contribuição da participação de familiares no processo de reabilitação audiológica desses sujeitos e promover melhor adaptação dos mesmos em relação ao uso do aparelho auditivo. MÉTODOS: Fizeram parte da pesquisa 30 indivíduos com presbiacusia da cidade de Cascavel (PR. A amostra foi divida em dois grupos, o grupo 1 (grupo controle foi constituído por 15 indivíduos que receberam uma única sessão de orientação e o grupo 2 (grupo experimental constituiu-se de 15 idosos que participaram de um programa de reabilitação fonoaudiológica fracionado em seis sessões. RESULTADOS: Foram encontradas diferenças estatisticamente significativas entre os grupos controle e experimental, quando comparadas as entrevistas dos pacientes e familiares, em aspectos como: manuseio da prótese auditiva, uso do telefone com a prótese auditiva e uso do diálogo em ambientes ruidosos. CONCLUSÕES: Neste estudo constatou-se que os idosos tiveram necessidade de acompanhamento fonoaudiológico após a adaptação do aparelho auditivo, pois sozinhos, muitas vezes, não conseguiam manusear a prótese auditiva e utilizá-la de maneira adequada.PURPOSE: The aim of this research consisted on developing a training program of communicative strategies for elderly people with auditory problems, users of individual hearing aids, and their families. Additionally, we had the aim to verify the contribution of family intervention during the process of auditory rehabilitation and to promote better adaptation of these individuals regarding the use of their hearing aids. METHODS: Thirty elderly subjects with presbycusis from the city of Cascavel (PR - Brazil took part in this research

  13. O cuidado nos centros municipais educacionais infantis em relação às infecções respiratórias agudas = The care in the municipal centers for children education about acute respiratory infections

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    Eloeth Kaliska Piva

    2012-01-01

    Full Text Available As infecções respiratórias agudas (IRA são causa de morbidade e mortalidade na infância, e sua crescente manifestação e transmissão nos centros educacionais infantis têm sugestionado maior atenção aos cuidados prestados nesses locais as crianças. Este estudo teve por objetivo conhecer as atitudes preventivas e curativas do cuidado proporcionado pelos monitores educacionais dos Centros Municipais Educacionais Infantis da Cidade de Cascavel - PR diante das IRA. Para tanto, realizamos entre novembro a dezembro de 2008, uma pesquisa qualitativa com 12 monitores educacionais, por meio da entrevista semi-estruturada gravada. Os aspectos emergentes das entrevistas foram pontuados e orientado na ótica do cuidado integral de José Ricardo de Carvalho Mesquita Ayres. As ações das monitoras educacionais em relação as IRA têm caráter curativo, sendo fundamentadas no cuidado técnico e entendimentos causais. Nessas ações faltam elementos que compõem o cuidado integral como o movimento, a interação, a reconstrução de identidades e alteridades, a não-causalidade, a plasticidade, a temporalidade e a responsabilidade. Diante disso, enfatiza-se a necessidade de preparar os monitores e as instituições para o cuidado integral. E, sobretudo, a articulação dos centros educacionais aos setores de saúde promovendo a aplicação de estratégias para o controle, prevenção e promoção da saúde das crianças.Acute respiratory infections are the causes of morbidity and mortality during infancy and childhood. Their increasing manifestation and transmission in day-care centers have demanded greater attention for children’ care in such institutions. Current research investigates prevention and care activities provided by educational monitors of day-care center in Cascavel, Paraná State, Brazil, with regard to acute respiratory infections. A qualitative research was undertaken with 12 educational monitors between November and December 2008

  14. Spatial variability of soil physical attributes and its effects on wheat yield / Variabilidade espacial de atributos físicos do solo e seus efeitos sobre a produtividade do trigo

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    Miguel Angel Uribe-Opazo

    2009-07-01

    Full Text Available The objective of this paper was to study the spatial variability influence of soil physical attributes in wheat yield. The geostatistics techniques used were semivariogram and contour maps produced by interpolation through usual kriging representing the spatial variability of the soil physical attributes: soil water content, soil total porosity, soil compaction degree and soil resistance to penetration.. Experimental data were at a Rhodic Ferralsol from the Agricultural Engineering Experimental Nucleus at UNIOESTE – Cascavel Campus. Soil physical attributes and wheat yield maps presented a variability standard regarding distribution in experimental area, showing low wheat yield where there was high soil compaction degree, low soil total porosity and low soil water content also presenting good wheat yield where low and medium soil resistance to penetration, medium and high soil water content and low soil compaction degree occurred. Among the studied soil physical attributes studied, soil resistance to penetration was the best attribute in correlation with wheat yield. The second best attribute was soil compaction degree. This paper enabled us to understand in a better way the spatial dependence structure of the studied attributes, as well as the concepts and geostatistics applications.Este trabalho teve como objetivo estudar a influência da variabilidade espacial dos atributos físicos do solo sobre a produtividade de trigo. Foram utilizadas técnicas de geoestatística como a construção de semivariogramas e confecção de mapas temáticos, produzidos por interpolação por krigagem de atributos físicos do solo, tais como: conteúdo gravimétrico de água no solo, porosidade total, grau de compactação e resistência do solo à penetração. Os dados experimentais foram obtidos em uma área de Latossolo Vermelho-Escuro, pertencente ao Núcleo Experimental de Engenharia Agrícola da Universidade Estadual do Oeste do Paraná – Campus de

  15. terapêutica

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    Marisa Ines Tomazzoni

    2006-01-01

    Full Text Available Se trata de un estudio etnobotánico e investigación de opinión, que tuvo como objetivo ampliar el conocimiento sobre el uso de las plantas medicinales en la comunidad de la Municipalidad de Cascavel-PR, con miras a apoyar en la implantación de los fitoterápicos en la red pública de la salud. La muestra correspondió a 50 familias del área de la jurisdicción de una Unidad Básica de Salud, seleccionadas mediante una muestra estratificada proporcional. Para la obtención de los datos se optó por una investigación de carácter exploratório-descriptivo, a través de la entrevista semiestructurada. Como resultado, se observo que el 96% de la población albo manifestó el uso de la plantas; fueron registradas 271 referencias etnobotánicas, englobando 75 etnoespecies; 40 propiedades terapéuticas estaban relacionadas al uso doméstico. El uso de las plantas es bastante difundido y la transferencia eel conocimiento etnobotánico sigue los padrones de las comunidades trradicionales, no existiendo bloqueos en éste proceso. Se constató también, que las plantas medicinales para la comunidad representan un factor importante para la manutención de las condiciones de la salud, formando parte de un saber local preservado.

  16. Body image dissatisfaction and anthropometric indicators in male children and adolescents.

    Science.gov (United States)

    Ferrari, E P; Minatto, G; Berria, J; Silva, S F Dos S; Fidelix, Y L; Ribeiro, R R; Santos, K D; Petroski, E L

    2015-10-01

    The aim of this study was to investigate the association between body image dissatisfaction and body mass index (BMI) and body fat percentage (BF%) and to identify which of these anthropometric indicators are more strongly associated, and finally to estimate the prevalence of overweight and high body adiposity in male children and adolescents, according to maturational stages. Overall, 1499 students aged from 7 to 17 years from Cascavel, PR, Brazil, were evaluated. Body image was self-rated through the body silhouette scale. Body weight, height and triceps and subscapular skinfolds were measured and BMI and BF% were calculated. Sexual maturity was self-assessed by the development of pubic hair. Data analysis used the Fisher exact test, the χ(2)-test and multinomial logistic regression. Body image dissatisfaction because of excess weight was associated with BMI and BF%, whereas in prepubertal students, this association did not remain in the adjusted analysis. In pubescent students, both BMI (odds ratio (OR)=5.25, confidence interval (CI) 95%=3.06-9.01) and BF% (OR=2.42, CI 95%=1.60-3.66), and in post-pubescent students for BMI (OR=3.77, CI 95%=1.33-10.70), the association remained. Body image dissatisfaction because of thinness was associated only with BF% in pubescent (OR=0.50, CI 95%= 0.33-0.75) and post-pubescent students (OR=0.38, CI 95%= 0.16-0.94). Body image dissatisfaction was associated with BMI and BF%, especially in pubescent and post-pubescent students.

  17. Inoculation with Azospirillum, associated with nitrogen fertilization in maize

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    Maria do Carmo Lana

    2012-06-01

    Full Text Available The biological nitrogen fixation is an alternative to supply the nitrogen needed for maize. The objective of this study was to evaluate the development and yield of maize in response to inoculation with Azospirillum associated with nitrogen fertilization. We conducted two field experiments in the summer harvest, the first in the 2000/2001 crop year in the region of Marechal Cândido Rondon, under conventional tillage, and second in the 2002/2003 agricultural year in the region of Cascavel, under no tillage. The experimental design in both experiments was a randomized complete block, with four replications, 2x2x2 factorial, with two levels of nitrogen at sowing (zero and 20 kg ha-1, two levels of inoculum (zero and 200 g ha-1 and two levels of nitrogen in topdressing (zero and 100 kg ha-1. There was evaluated the height of ear insertion, total plant height, leaf N content, shoot dry biomass and grain yield. The height of ear insertion and total plant height were not influenced by the factors under study. Nitrogen fertilization at sowing increased the leaf N content, causing the opposite effect when combined with inoculation. Inoculation with Azospirillum in the absence of nitrogen, provide productivity increases of 15.4% and 7.4% for 2000/2001 and 2002/2003 crops, respectively. The inoculation provided productivity similar to that obtained with 100 kg ha-1 in topdressing in crop 2000/2001, while in association with the topdressing, reduced productivity and shoot dry biomass in crop 2002/2003.

  18. Directory of Open Access Journals (Sweden)

    Giseli Minatto

    2014-01-01

    Full Text Available We aimed to estimate the prevalence of high body adiposity and its association with musculoskeletal fitness in male children and adolescents according to socioeconomic status. A cross-sectional epidemiological study was carried out with 1,531 school children (6-17 years old attending public schools in Cascavel, state of Paraná, Brazil. Body adiposity was estimated based on skinfold thickness. Information was also collected on chronological age, socioeconomic status, sexual maturation, performance in physical tests such as sit and reach, 1-minute sit-up, stationary long jump and Shuttle run. Statistical analyses were performed (Student’s unpaired t test and Poisson regression taking into consideration socioeconomic status (high and low+middle, with p<0.05. High body adiposity was observed in 30.4% of the sample, and was greater (p<0.05 among those of high socioeconomic status (33.3% vs 28.3%. After adjustment for all variables, high body adiposity was associated with low abdominal resistance (PR=1.44; CI95%=1.05-1.99 and lower limb power (PR=2.09; CI95%=1.46-1.98 in the low socioeconomic status group. In the high socioeconomic status group, the outcome was associated with low abdominal resistance (PR=1.72; CI95%=1.17-2.51 and with intermediate (PR=2.83; CI95%=1.76-4.55 and low (PR=3.90; CI95%=2.38-6.38 lower limb power. In both socioeconomic levels, lower musculoskeletal fitness (abdominal resistance and lower limb power was associated with high body adiposity. However, the magnitude of the association between muscular capacity and high body adiposity seems to differ according to socioeconomic status.

  19. Rede e apoio social familiar no seguimento do recém-nascido pré-termo e baixo peso ao nascer

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    Débora Falleiros de Mello

    2010-03-01

    Full Text Available Um dos aspectos ligados à sobrevida do recém-nascido (RN pré-termo (PT e de baixo peso ao nascer (BPN é relativo ao período após a alta hospitalar. Este estudo tem por objetivo identificar a rede social e o apoio social de famílias com filho PT e BPN egresso da UTI neonatal, a partir da utilização dos instrumentos genograma e ecomapa com estas famílias no município de Cascavel-Paraná. É um estudo descritivo, com abordagem qualitativa, sendo que a partir dos relatos de seis famílias com filhos egressos de UTIN, acompanhadas por meio de visitas domiciliares nos três primeiros meses após a alta hospitalar, obtiveram-se dados que foram agrupados ao redor de três temas: A rede social como integrante do seguimento do PT e BPN no contexto da família; O apoio social nas experiências das famílias com bebê PT e BPN; A vulnerabilidade e o contexto da rede e do apoio social. Emergiu no estudo, um conjunto de atores e cenários que fazem parte da rede ligada às famílias no seu cotidiano. A atuação da enfermagem ganha mais sentido com o conhecimento dessa rede, que proporciona uma integração entre saberes práticos e saberes técnicos.

  20. Alelopatia de Camelina sativa Boiss. (Brassicaceae sobre a germinação e desenvolvimento inicial de Bidens pilosa (L. e Glycine max (L. Merr

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    Jéssica da Silva

    2011-09-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2011v24n4p17 O presente trabalho buscou avaliar a possível presença de potencial alelopático na camelina, bem como o seu efeito sobre a germinação e o desenvolvimento inicial de plântulas de soja e picão-preto, a fim de verificar a possibilidade do cultivo dela com a soja no sistema de rotação de culturas e de seu uso como herbicida. Os experimentos foram realizados no Laboratório de Fisiologia Vegetal da Universidade Estadual do Oeste do Paraná, Cascavel, Paraná. O delineamento foi inteiramente casualizado e as avaliações realizadas diariamente. Os parâmetros avaliados foram: porcentagem de germinação, tempo e velocidade média de germinação e comprimento médio de raiz. Os dados obtidos foram submetidos ao Teste F e as médias comparadas pelo Teste de Tukey, a 5% de probabilidade. Os resultados obtidos comprovam a presença de potencial alelopático na camelina. Foi verificado que esta espécie pode ser considerada uma opção para o cultivo com a soja devido à interferência alelopática positiva provocada na cultura e ainda, que ela apresenta capacidade para utilização no controle de plantas invasoras como o picão-preto, já que atuou atrasando o desenvolvimento das plântulas testadas.

  1. Energy cost simulations for heating in greenhouses for five cities in Brazil; Simulacoes do custo de energia para calefacao em casas de vegetacao para cinco cidades brasileiras

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Celso Eduardo Lins de [Universidade Estadual do Oeste do Parana (UNIOESTE), Cascavel, PR (Brazil)], e-mail: celsooli@unioeste.br; La Plaza, Saturnino de; Garcia, Jose Luis [Universidad Politecnica de Madrid (UPM), Madrid, (Spain). Escuela Tecnica Superior de Ingenieros Agronomos

    2004-07-01

    Using the values of the global heat loss coefficient (U) calculated with the equations of the lineal model developed by OLIVEIRA (2003), and with the 30 year-old values, the energy can be calculated by square meter that would be necessary to obtain a temperature set point of 15 deg C, to a greenhouse of 132 m{sup 2}. They were chosen the cities of Cascavel, Curitiba, Florianopolis, Porto Alegre and Sao Paulo. To each place the medium day of the months was used to determine the convenience of the heating that the minimum average went under 15 deg C. Observing the medium minimum temperatures is easy to verify that the support of alone heat would be justified for a crop with high energy need and excellent commercial value, as it can be the case of the crop of some ornamental species. The value of estimated energy was multiplied by the Euros cost for Kwh for fuel-oil, natural gas and diesel, being simulate the heating use for heat fan and radiant floor, combined or not with two techniques of energy saving: use a double layer or plastic tunnel over the crop. Should even consider that in all the chosen places frost risk exists, what can influence in the profitability of many crops. The city of Florianopolis went to that presented smaller thermal solicitations and, therefore smaller costs, the city of Curitiba already demonstrated the needs of a support system for eight months and, therefore, larger costs. The technical more efficient it is the use of combined radiant floor with plastic tunnel, being the combustible more economic is fuel-oil. (author)

  2. VIABILIDADE AMBIENTAL E FINANCEIRA EM UMA MICROEMPRESA FAMILIAR: VALE O INVESTIMENTO?/ Environmental and financial feasibility in a family microenterprise: is the investment worth?

    Directory of Open Access Journals (Sweden)

    Gustavo Yuho Endo

    2016-08-01

    Full Text Available Este artigo objetivou apresentar os custos de adequação às exigências da legislação ambiental no licenciamento de uma oficina mecânica familiar no município de Cascavel, estado do Paraná, atuando na melhoria dos processos de gerenciamento de resíduos sólidos produzidos pela empresa e analisando se os custos de adequação são viáveis financeira e ambientalmente. O trabalho utilizou o método de estudo de caso intrínseco e uma pesquisa de toda legislação que afeta o setor, com posterior verificação das mudanças necessárias à legislação e precificação dessas alterações. Em paralelo foi desenvolvido uma pesquisa da disposição a pagar a mais por produtos/serviços ambientalmente corretos, o que resultou em valores percentuais aplicados à fórmula. Como resultado pode-se verificar que diante dos custos auferidos e fazendo-se o cálculo de viabilidade ambiental e financeira, o empresário obtém um retorno sobre o investimento que se justifica, sendo que além do retorno financeiro, ainda evita as sanções pela não adequação à legislação vigente. Conclui-se, portanto, que além da questão ambiental, no caso em estudo, é também financeiramente viável o investimento nas adequações propostas, mesmo sendo esta uma empresa de pequeno porte e familiar.

  3. Association between cardiorespiratory fitness and body fat in girls

    Science.gov (United States)

    Minatto, Giseli; de Sousa, Thiago Ferreira; de Carvalho, Wellington Roberto Gomes; Ribeiro, Roberto Régis; Santos, Keila Donassolo; Petroski, Edio Luiz

    2016-01-01

    Abstract Objective: To estimate the prevalence of low cardiorespiratory fitness and its association with excess body fat, considering the sexual maturation and economic level in female adolescents. Methods: Cross-sectional, epidemiological study of 1223 adolescents (10-17 years) from the public school system of Cascavel, PR, Brazil, in 2006. We analyzed the self-assessed sexual maturation level (prepubertal, pubertal and post-pubertal), the economic level (high and low) through a questionnaire and body fat (normal and high) through triceps and subscapular skinfolds. The 20-meter back-and-forth test was applied to estimate maximum oxygen consumption. Cardiorespiratory fitness was assessed according to reference criteria and considered low when the minimum health criterion for age and sex was not met. Chi-square test and logistic regression were applied, with a significance level of 5%. Results: The prevalence of low cardiorespiratory fitness was 51.3%, being associated with all study variables (p<0.001). At the crude analysis, adolescents with high body fat were associated with low cardiorespiratory fitness, when compared to those with normal body fat (OR=2.76; 95%CI: 2.17-3.52). After adjustment by sexual maturation, this association remained valid and showed an effect that was 1.8-fold higher (95%CI: 1.39-2.46) and after adjusting by economic level, the effect was 1.9-fold higher (95%CI: 1.45-2.61). Conclusions: Approximately half of the assessed girls showed unsatisfactory levels of cardiorespiratory fitness for health, which was associated with high body fat, regardless of sexual maturation level and economic level. Effective public health measures are needed, with particular attention to high-risk groups. PMID:27131896

  4. AVALIAÇÃO E COMPARAÇÃO ENTRE KITS LABORATORIAIS “D-DÍMERO” COMO DIAGNÓSTICO DE TROMBOSE VENOSA PROFUNDA

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    Rafaela Natiéli LIMA

    2015-12-01

    Full Text Available O presente estudo teve como objetivos realizar análise comparativa entre kits laboratoriais D-Dímero para Trombose Venosa Profunda (TVP pelos métodos de Imunoturbidimetria (metodologia 1 e Quimiluminescência (metodologia 2, ambos da marca Siemens, e comparar o resultado das análises realizadas, com o diagnóstico clínico confirmatório do paciente, analisando seus prontuários. A amostra foi constituída por uma população de 95 voluntários que realizaram o teste laboratorial D-Dímero, em um laboratório de análises clínicas da cidade de Cascavel-PR. Os resultados foram comparados através dos parâmetros para validação sorológica, através dos cálculos de especificidade, o qual foi de 33% em ambos os métodos, sensibilidade, o qual foi de 89% e 90%, e a eficiência de 68% e 69%, respectivamente para as metodologias 1 e 2. O valor preditivo positivo (VPP foi de 69% em ambos os testes, o valor preditivo negativo (VPN foi de 33%, a prevalência foi de 62%, e a prevalência sorológica foi de 81%. Conclui-se assim que, os testes analisados são pouco específicos para TVP, não podendo ser utilizados como método diagnóstico, mas sim para excluir prováveis suspeitas de tromboembolismo venoso.

  5. PROFUNDIDADE DO LENÇOL FREÁTICO NO CULTIVO DE CANOLA

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    Eduardo de De Rossi

    2015-12-01

    Full Text Available Desde os primórdios do surgimento da agricultura os níveis do lençol freático são parte importante na produção de alimentos, já nos dias atuais, esta produção se abrange também para setores como o energético e ambiental. A canola (Brassica napus L., no Brasil, teve nos últimos anos um aumento expressivo de produção devido ao grande interesse, principalmente alimentar e energético. Um fator que poderia facilitar o seu plantio é o conhecimento de sua necessidade hídrica, em relação à profundidade do lençol freático. Neste sentido, este trabalho teve como objetivo avaliar o efeito da variação de níveis de lençol freático no crescimento inicial da canola. O trabalho foi realizado em lisímetros com diâmetro de 20 cm em casa de vegetação da Unioeste, campus Cascavel. O delineamento experimental foi inteiramente casualizado com seis tratamentos e seis repetições de profundidade do lençol freático: 0,1; 0,2; 0,3; 0,4; 0,5 e 0,6 m. Avaliou-se a altura de planta, número de folhas, área foliar, comprimento radicular, massa fresca e seca.

  6. Estudo retrospectivo das complicações intra-operatórias na cirurgia de revascularização do miocárdio

    Directory of Open Access Journals (Sweden)

    Ariana Rodrigues Silva Carvalho

    2008-12-01

    Full Text Available As complicações intra-operatórias na cirurgia de revascularização miocárdica (CRM quando reconhecidas previamente podem guiar os cuidados prestados na sala operatória, dando continuidade na Unidade de Terapia Intensiva e durante toda internação. Quando a equipe conhece essas complicações sofridas pelo indivíduo poderá prever e prover cuidados mais específicos na seqüência de sua evolução cirúrgica. O objetivo desse estudo foi investigar a freqüência e tipos de complicações observadas no intra-operatório da CRM. Trata-se de um estudo retrospectivo, descritivo, transversal, baseado em dados secundários, com amostra intencional de 123 sujeitos. A coleta de dados realizou-se por meio do acesso ao banco de dados do Instituto de Moléstias Cardiovasculares do Oeste do Paraná, em Cascavel. Os dados foram analisados no programa computadorizado Statistic, versão 6,0. Os tipos de complicações observadas nesse período foram: cardiovasculares, pulmonares, alteração nos vasos do enxerto e sangramento. Dentre essas, a que obteve maior freqüência foi “alteração nos vasos do enxerto”, em 33 (26,8% casos. As complicações no intra-operatório podem não ser passíveis de prevenção quando consideradas as características individuais de cada sujeito, entretanto, todos os esforços precisam ser direcionados para que o evento, quando ocorrer, seja bem atendido, evitando dessa forma, complicações adicionais maiores.

  7. Frequency of potential interactions between drugs in medical prescriptions in a city in southern Brazil

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    Genici Weyh Bleich

    Full Text Available CONTEXT AND OBJECTIVE: Drug interactions form part of current clinical practice and they affect between 3 and 5% of polypharmacy patients. The aim of this study was to identify the frequency of potential drug-drug interactions in prescriptions for adult and elderly patients. TYPE OF STUDY AND SETTING: Cross-sectional pharmacoepidemiological survey in the Parque Verde housing project, municipality of Cascavel, Paraná, Brazil, between December 2006 and February 2007. METHODS: Stratified cluster sampling, proportional to the total number of homes in the housing project, was used. The sample consisted of 95 homes and 96 male or female patients aged 19 or over, with medical prescriptions for at least two pharmaceutical drugs. Interactions were identified using DrugDigest, Medscape and Micromedex softwares. RESULTS: Most of the patients were female (69.8%, married (59.4% and in the age group of 60 years or over (56.3%, with an income less than or equal to three minimum monthly salaries (81.3% and less than eight years of schooling (69.8%; 90.6% of the patients were living with another person. The total number of pharmaceutical drugs was 406 (average of 4.2 medications per patient. The drugs most prescribed were antihypertensives (47.5%. The frequency of drug interactions was 66.6%. Among the 154 potential drug interactions, 4.6% were classified as major, 65.6% as moderate and 20.1% as minor. CONCLUSION: The high frequency of drug prescriptions with a potential for differentiated interactions indicates a situation that has so far been little explored, albeit a reality in household surveys.

  8. Methodology for quantifying the technological level of energy in electric motors; Metodologia para quantificacao do nivel tecnologico energetico de motores eletricos

    Energy Technology Data Exchange (ETDEWEB)

    Konopatzki, Evandro Andre; Bianchessi, Joel Marcos [Faculdade Assis Gurgacz (FAG), Cascavel, PR (Brazil); Oliveira, Celso Eduardo Lins de [Universidade de Sao Paulo (FZEA-USP), Pirassununga, SP (Brazil). Fac. de Zootecnia e Engenharia de Alimentos; Konopatzki, Angelica da Silva Lima [Faculdade de Ciencias Aplicadas de Cascavel (FACIAP), PR (Brazil). Curso de Ciencia da Computacao; Marim, Cesar [Universidade Estadual do Oeste do Parana (UNIOESTE), Cascavel, PR (Brazil)

    2006-07-01

    The market competition created in agricultural industry because of its insertion in the domestic and foreign markets, verified by the increase in GDP share of agribusiness in the Brazilian GDP, led us to have better control of costs and inputs. Thus the final value of products sold by these agribusinesses can download increasing the space in the consumer market. Electricity is one of the inputs now being studied and this paper presents a model for determining the coefficients that allow the comparison of different existing technologies for electric motors inserting an indicator of technological innovation called Technological Level Energy (NTE). The NTE was developed and tested in Storage Units of Grain (UAG) in the region of Cascavel - Parana where data were collected in six UAG, analyzed in medium weighted, had levels of different energy technology development. The main elements used to create the NTE are the number of engines installed in UAG, the lifetime of the engine considered from its manufacture and motor plate data such as income and the power factor. As a comparative process, each region can create your NTE and analyze your situation in a national context. The UAG will use the NTE to technological innovation and planning projects such as depreciation, financing and cross-examination of financial resources for the exchange of electric motors. Thus the NTE will aid in comparative indication of the timing for replacement of electric motors. Moment from which the energy demand, according to the obsolescence of equipment, are considered excessive compared to other more modern UAG. (author)

  9. Prevalence of serological markers of hepatitis B virus in pregnant women from Paraná State, Brazil

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    D.A. Bertolini

    2006-08-01

    Full Text Available The prevalence of hepatitis B virus (HBV in Brazil increases from South to North but moderate to elevated prevalence has been detected in the Southwest of Paraná State. The prevalence of serological markers of HBV was evaluated in 3188 pregnant women from different counties in Paraná State and relevant epidemiological features were described. The prevalence of HBV markers in pregnant women for the state as a whole was 18.5% (95% CI = 17.2-19.9, ranging from 7.2% in Curitiba to 38.5% in Francisco Beltrão. The endemicity of HBV marker prevalence in pregnant women was intermediate in Cascavel, Foz do Iguaçu, and Francisco Beltrão, and low in Curitiba, Londrina, Maringá, and Paranaguá. Multiple logistic regression showed that HBV marker prevalence increased with age, was higher among black women, among women of Italian and German descent, and among women who had family members in neighboring Rio Grande do Sul State. Univariate analysis showed that HBV marker prevalence was also higher among women with no education or only primary education, with a lower family income and whose families originated from the South Region of Brazil. Pregnant women not having positive HBV markers (anti-HBc, HBsAg or anti-HBs detected by ELISA corresponded to 73.7% of the population studied, implying that HBV vaccination needs to be reinforced in Paraná State. The highest prevalence was found in three counties that received the largest number of families from Santa Catarina and Rio Grande do Sul, where most immigrants were of German or Italian ascendance. This finding probably indicates that immigrants that came to this area brought HBV infection to Southwestern Paraná State.

  10. A rational design for the nanoencapsulation of poisonous animal venoms in liposomes prepared with natural phospholipids.

    Science.gov (United States)

    da Costa, Maria Helena Bueno; Sant'Anna, Osvaldo A; Quintilio, Wagner; Schwendener, Reto Albert; de Araujo, Pedro Soares

    2012-11-01

    Liposomes have been used since the 1970's to encapsulate drugs envisaging enhancement in efficacy and therapeutic index, avoidance of side effects and increase in the encapsulated agent stability. The major problem when encapsulating snake venoms is the liposomal membrane instability caused by venom phospholipases. Here the results obtained encapsulating Crotalus durissimus terrificus and a pool of Bothropic venoms within liposomes (LC and LB, respectively) used to produce anti-venom sera are presented. The strategy was to modify the immunization protocol to enhance antibody production and to minimize toxic effects by encapsulating inactivated venoms within stabilized liposomes. Chemically modified venoms were solubilized in a buffer containing an inhibitor and a chelating agent. The structures of the venoms were analyzed by UV, CD spectroscopy and ELISA. In spite of the differences in the helical content between natural and modified venoms, they were recognized by horse anti-sera. To maintain long-term stability, mannitol was used as a cryoprotectant. The encapsulation efficiencies were 59 % (LB) and 99 % (LC), as followed by filtration on Sephacryl S1000. Light scattering measurements led us to conclude that both, LB (119 ±47 nm) and LC (147±56 nm) were stable for 22 days at 4 °C, even after lyophilization. Genetically selected mice and mixed breed horses were immunized with these formulations. The animals did not show clinical symptoms of venom toxicity. Both, LB and LC enhanced by at least 30 % the antibody titers 25 days after injection and total IgG titers remained high 91 days after immunization. The liposomal formulation clearly exhibited adjuvant properties.

  11. Peripheral Sensitization Increases Opioid Receptor Expression and Activation by Crotalphine in Rats

    Science.gov (United States)

    Zambelli, Vanessa Olzon; Fernandes, Ana Carolina de Oliveira; Gutierrez, Vanessa Pacciari; Ferreira, Julio Cesar Batista; Parada, Carlos Amilcar; Mochly-Rosen, Daria; Cury, Yara

    2014-01-01

    Inflammation enhances the peripheral analgesic efficacy of opioid drugs, but the mechanisms involved in this phenomenon have not been fully elucidated. Crotalphine (CRP), a peptide that was first isolated from South American rattlesnake C.d. terrificus venom, induces a potent and long-lasting anti-nociceptive effect that is mediated by the activation of peripheral opioid receptors. Because the high efficacy of CRP is only observed in the presence of inflammation, we aimed to elucidate the mechanisms involved in the CRP anti-nociceptive effect induced by inflammation. Using real-time RT-PCR, western blot analysis and ELISA assays, we demonstrate that the intraplantar injection of prostaglandin E2 (PGE2) increases the mRNA and protein levels of the µ- and κ-opioid receptors in the dorsal root ganglia (DRG) and paw tissue of rats within 3 h of the injection. Using conformation state-sensitive antibodies that recognize activated opioid receptors, we show that PGE2, alone does not increase the activation of these opioid receptors but that in the presence of PGE2, the activation of specific opioid receptors by CRP and selective µ- and κ-opioid receptor agonists (positive controls) increases. Furthermore, PGE2 down-regulated the expression and activation of the δ-opioid receptor. CRP increased the level of activated mitogen-activated protein kinases in cultured DRG neurons, and this increase was dependent on the activation of protein kinase Cζ. This CRP effect was much more prominent when the cells were pretreated with PGE2. These results indicate that the expression and activation of peripheral opioid receptors by opioid-like drugs can be up- or down-regulated in the presence of an acute injury and that acute tissue injury enhances the efficacy of peripheral opioids. PMID:24594607

  12. Atividade antiinflamatória do extrato aquoso de Arrabidaea chica (Humb. & Bonpl. B. Verl. sobre o edema induzido por venenos de serpentes amazônicas Anti-inflammatory activity of the aqueous extract of Arrabidaea chica (Humb. & Bonpl. B. Verl. on the self-induced inflammatory process from venoms amazonians snakes

    Directory of Open Access Journals (Sweden)

    Denys Paixão Costa de Oliveira

    2009-06-01

    Full Text Available O estudo investigou o efeito antiinflamatório do extrato aquoso da Arrabidaea chica (Humb. & Bonpl. B. Verl., Bignoniaceae, popularmente conhecida como "crajiru", sobre o edema induzido por venenos de serpentes amazônicas dos gêneros Brothrops e Crotalus, em camundongos albinos, por via oral, intraperitoneal e subcutânea. O efeito anti-edematogênico foi avaliado pela medição do diâmetro dos coxins das patas posteriores, sendo medidos as 1, 3, 6, 12 e 24 horas, para B. atrox e 1, 3 e 6 horas para Crotalus durissus ruruima, e também avaliado por histopatologia. O estudo mostrou que o efeito inibitório do extrato aquoso para o gênero Bothrops, pelas vias subcutânea e intraperitoneal (12 horas foi de 55,87% e 65,70%, respectivamente. Para o gênero Crotalus o efeito inibitório do extrato pela via subcutânea após 3 horas foi de 33,55% e após 6 horas de 79,81%. Pela via intraperitoneal após 3 horas foi de 48,02% e após 6 horas de 92,52%. Na análise histopatológica, o infiltrado de granulócitos e a miocitólise foram os efeitos inflamatórios inibidos mais significativamente. Os resultados sugerem a presença no extrato aquoso de A. chica de substâncias com atividade inibitória sobre os efeitos inflamatórios dos venenos das serpentes Bothrops atrox e Crotalus durissus ruruima.The study investigated the anti-inflammatory effects of the aqueous extract (EAq, the Arrabidaea chica (Humb. & Bonpl. B. Verl., Bignoniaceae, known as the "crajiru", tested on the paw oedema induced in inflammatory process by venoms Amazon snakes from the Bothrops and Crotalus species, on albino mice. This process was done by an oral, intraperitoneal and subcutaneous way. The cushions thickness was measured at time breaks of 1, 3, 6, 12 and 24 hours, Bothrops genus and 1, 3 and 6 hours, Crotalus genus, and a histopathologic analysis was made. The Bothrops genus, was used as an inhibitory effect for the subcutaneous and intraperitoneal way (12 hours, and it

  13. Eficacia experimental de anticuerpos IgY producidos en huevos, contra el veneno de la serpiente peruana Bothrops atrox Experimental efficacy of IgY antibodies produced in eggs against the venom of the Peruvian snake Bothrops atrox

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    Julio C. Mendoza

    2012-03-01

    Full Text Available Objetivos. Desarrollar un protocolo de inmunización para producir inmunoglobulinas IgY de origen aviar contra el veneno de la serpiente peruana Bothrops atrox y evaluar la capacidad neutralizante. Materiales y métodos. Se inmunizaron seis gallinas de postura de la raza hy line brown con 500 μg/dosis de veneno de B. atrox en un periodo de dos meses. Cada semana, los huevos fueron colectados para el aislamiento de inmunoglobulinas IgY a partir de la yema, usando dos pasos consecutivos con αcido caprνlico y sulfato de amonio. La detecciσn de anticuerpos se realizσ por inmunodifusiσn doble mientras que el tνtulo y reactividad cruzada se determinaron por las técnicas de ELISA y Western blot. El cálculo de DL50 y de la DE50 del antiveneno IgY producido se realizó utilizando el método de Probits. Resultados. La masa de anticuerpos aislados fue de 8,5 ± 1,35 mg de IgY/mL de yema. Asimismo, la DE50 del antiveneno aviar fue calculada en 575 μL de antiveneno/mg de veneno. Adicionalmente, los ensayos de reactividad cruzada mostraron que el veneno de B. atrox comparte mas epνtopes comunes con el veneno de B. brazili (47% que con otros veneno del mismo género, en tanto que los venenos de Lachesis muta (19% y Crotalus durissus (12% mostraron una baja reactividad cruzada. Conclusiones. Se ha obtenido IgY purificada contra el veneno de B. atrox con capacidad neutralizante y se ha demostrado su utilidad como herramienta inmunoanalítica para evaluar la reactividad cruzada con venenos de otras especies.Objectives. To develop an immunization protocol in order to produce avian IgY immunoglobulins against Bothrops atrox Peruvian snake venom and to evaluate its neutralizing capacity. Materials and methods. Six Hy Line Brown hens were immunized each two weeks using 500μg/doses of B. atrox venom in a period of two months. Each week, eggs were collected for IgY isolation from yolk using two consecutive steps with caprilic acid and ammonium sulfate

  14. Development of snake-directed antipredator behavior by wild white-faced capuchin monkeys: I. Snake-species discrimination.

    Science.gov (United States)

    Meno, Whitney; Coss, Richard G; Perry, Susan

    2013-03-01

    Young animals are known to direct alarm calls at a wider range of species than adults. Our field study examined age-related differences in the snake-directed antipredator behavior of infant, juvenile, and adult white-faced capuchin monkeys (Cebus capucinus) in terms of alarm calling, looking behavior, and aggressive behavior. In the first experiment, we exposed infant and juvenile white-faced capuchins to realistic-looking inflatable models of their two snake predators, the boa constrictior (Boa constrictor) and neotropical rattlesnake (Crotalus durissus) and a white airplane as a novel control. In the second experiment, infants, juveniles, and adults were presented photographic models of a coiled boa constrictor, rattlesnake, indigo snake (Drymarchon corais), a noncapuchin predator, and a white snake-like model. We found that antipredator behavior changed during the immature stage. Infants as young as 4 months old were able to recognize snakes and display antipredator behavior, but engaged in less snake-model discrimination than juveniles. All age classes exhibited a lower response to the white snake-like model, indicating that the absence of color and snake-scale patterns affected snake recognition. Infants also showed a higher level of vigilance after snake-model detection as exhibited by a higher proportion of time spent looking and head cocking at the models. Aggressive antipredator behavior was found in all age classes, but was more prevalent in juveniles and adults than infants. This study adds to the knowledge of development of antipredator behavior in primates by showing that, although alarm calling behavior and predator recognition appear at a very young age in capuchins, snake-species discrimination does not become apparent until the juvenile stage.

  15. Novo método de dosagem de soros antipeçonhentos em camundongos lactentes: I - dosagem do soro anticrotálico

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    Bruno Soerensen

    1984-08-01

    Full Text Available A atividade biológica dos soros antipeçonhentos pode ser determinada "in vivo" em pombos, coelhos, cobaios e camundongos adultos, não existindo, entretanto, nenhum método que possa ser recomendado internacionalmente. Estudou-se comparativamente aos métodos tradicionais de dosagem de soros em pombos e camundongos adultos, a validade do uso de camundongos lactentes de 6 a 7 dias, pesando 4 a 5g, inoculados pela via subcutânea. Inicialmente foi determinada a toxidez do veneno de Crotalus durissus terríficus através do estudo da sintomatologia do envenenamento e da atividade letal. O estudo comparativo dos três métodos forneceu maior concordância de resultados em DE100 entre as dosagens realizadas com camundongos lactentes e adultos. A DE100 e DL50 determinadas em camundongos lactentes forneceu resultados mais constantes que os dos métodos de camundongos adultos e pombos. O uso deste novo método permite eliminar a dificuldade de obtenção do atual animal de prova; a utilização de um maior número de animais por ponto de avaliação biológica, possibilitando uma maior precisão e conseguindo-se uma uniformidade nas características exigidas neste tipo de dosagem como peso, idade e linhagem, visando a reprodução sistemática dos resultados.

  16. Insuficiência renal aguda secundária a acidentes ofídicos botrópico e crotálico. Análise de 63 casos

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    Carlos Faria Santos Amaral

    1986-08-01

    Full Text Available Sessenta e três pacientes com insuficiência renal aguda secundária a acidente ofídico foram tratados no CTI do Hospital das Clínicas da UFMG. Em 32 pacientes (51% o acidente foi produzido por serpentes do gênero Bothrops (grupo bio-trópico e em 32 pacientes (49% pela cascavel sul-americana (grupo crotálico. As principais complicações apresentadas pelos pacientes foram a uremia (100% dos casos, hiperpotassemia (89% dos casos, anemia (78% dos casos, infecção urinária (37% dos casos, hiper-hidratação (17% dos casos, parada cardíaca (14% dos casos e edema agudo dos pulmões (11% dos casos. Cinco pacientes do grupo crotálico (16% tiveram insuficiência respiratória aguda atribuída à ação neurotóxica do veneno, quatro dos quais se recuperaram completamente. Sete pacientes do grupo botrópico (22% tiveram necrose cortical renal diagnosticada em cinco através da biópsia renal e em dois na necropsia. Quarenta e cinco pacientes (71% foram tratados com diálise peritoneal e a hemodiálise foi necessária em dois pacientes, um dos quais havia sido submetido a diálise peritoneal. Em 17 pacientes (27% o tratamento foi conservador. Cinqüenta e cinco pacientes receberam alta hospitalar, quatro dos quais com insuficiência renal crônica secundária a necrose cortical renal e oito (13% faleceram. Os óbitos foram atribuídos a edema pulmonar agudo em quatro pacientes, a estado de choque em dois pacientes e a coma e infecção respiratória após parada cardíaca em dois pacientes.

  17. Hepatitis B virus genotypes from European origin explains the high endemicity found in some areas from southern Brazil.

    Science.gov (United States)

    Bertolini, Dennis Armando; Gomes-Gouvêa, Michele Soares; Guedes de Carvalho-Mello, Isabel Maria Vicente; Carvalho-Mello, Isabel Maria Vicente Guedes de; Saraceni, Cláudia Patara; Sitnik, Roberta; Grazziotin, Felipe Gobbi; Laurino, Jomar Pereira; Laurindo, Jomar Pereira; Fagundes, Nelson Jurandi Rosa; Carrilho, Flair José; Pinho, João Renato Rebello

    2012-08-01

    Southern Brazil is considered an area of low Hepatitis B endemicity, but some areas of higher endemicity have been described in the Southwest of Paraná and Santa Catarina states. The aim of this study was to evaluate viral genotypes circulating throughout Paraná state. PCR amplification and partial sequencing of the S gene was carried out in 228 samples from HBsAg positive candidate blood donors. Samples have been collected in seven different counties (Cascavel, Curitiba, Foz do Iguaçu, Francisco Beltrão, Maringá, Londrina and Paranaguá). The most common HBV genotype in Paraná state was D (82.9%; 189/228), followed by A (14.1%; 32/228). Genotypes F (1.3%; 3/228), C (1.3%; 3/228) and H (0.4%; 1/228) were also found. Distribution of genotypes was different in the studied counties, but genotype D was the most frequent in all of them. In Francisco Beltrão, all studied samples belonged to genotype D. The high prevalence of HBV genotype D in South of Brazil is explained by the intense migration of settlers from Europeans countries. Subgenotypes A1 and A2 were identified circulating in all cities where HBV/A was found. As observed in other areas of Brazil, HBV/A1 is more frequent than the HBV/A2 in Paraná state and its presence was significantly larger in black and mulatto individuals. Genotype C was found only in individuals with Asian ancestry from Londrina and Maringá. Most HBV/F sequences identified in this study were classified as subgenotype F2a that was previously described in Brazil. The sole case of subgenotype F4 was from Foz do Iguaçu city, near to Northern Argentina, where F4 is highly prevalent. The single genotype H sample was from Curitiba. This is the first case of this genotype described in Brazil. Further studies should be carried out to determine if more genotype H samples can be found in other populations from Brazil.

  18. Tendências de consumo e preço de comercialização do pinhão (semente da Araucaria angustifolia (Bert. O. Ktze., no estado do Paraná / Trends of consumption and commercialization price of the Brazilian-pine nut – seed of Araucaria angustifolia (Bert. O. Ktze., in the state of Parana

    Directory of Open Access Journals (Sweden)

    Rafaelo Balbinot

    2008-09-01

    Full Text Available Este trabalho teve por objetivo estudar a evolução do preço e do volume de comercialização de pinhão, semente da Araucaria angustifolia (Bert. O. Ktze (Pinheiro-do-Paraná, no Estado do Paraná, no período de agosto1994 a fevereiro de 2002. Apesar do importante papel desempenhado poreste produto na economia, principalmente de famílias de baixa renda, e na cultura do Paraná, são quase inexistentes as informações e estudos sobre suas infl uências econômicas. Com a análise dos dados obtidos nas Centrais Estaduais de Abastecimento (CEASA´s de Curitiba, Maringá, Londrina, Foz do Iguaçu e Cascavel, os principais centros consumidoresdo Estado, foi possível observar uma grande variação dos preços, efeito da sazonalidade de sua produção. Também observou-se uma redução no preço real médio da saca de 20 kg do produto de R$25,00 em agosto de 1994 para R$8,47 em julho de 2002 , que corresponde a um preço nominal de R$18,88, sendo que, para manter o valor real de 1994, este teria que estar sendo comercializado a R$53,50. Quanto ao volume, aproximadamente 8,4 mil toneladas (cerca 80,7% do total, foramcomercializadas pelo CEASA de Curitiba, maior centro consumidor do Estado. A variação anual do volume comercializado chegou a 50% entre 2000 e 2001, sendo que a média anual de comercialização é de 1.300 toneladas, com tendência de manter esta média e a variação. Não foi detectada a presença de componente cíclico. Também não foi observada relação entre o volume comercializado e os preços praticados.

  19. Proposta de um Mapa Estratégico para uma Universidade Pública

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    Vinicius Abilio Martins

    2015-08-01

    Full Text Available A ferramenta de tradução da estratégia, medição e avaliação do desempenho organizacional chamado Balanced Scorecard (BSC vem sendo estudada de forma crescente desde a sua publicação em 1990. O formato inicialmente proposto é aplicado muito bem às empresas privadas. Porém, sua aplicação em organizações não pertencentes ao ramo privado não foi contemplado nos estudos iniciais. Este artigo tem por objetivo apresentar uma proposição de um mapa estratégico na forma do Balanced Scorecard para a Universidade Estadual do Oeste do Paraná (UNIOESTE, localizada na região Oeste daquele estado. Foram utilizados dados secundários relacionados ao Plano de Desenvolvimento Institucional, dentre outros documentos relacionados com a estratégia da instituição. Este estudo de caso se classifica como exploratório e qualitativo. A UNIOESTE possui, além da Reitoria, 5 campis localizados nas cidades de Cascavel, Toledo, Foz do Iguaçu, Marechal Cândido Rondon e Francisco Beltrão, além do Hospital Universitário do Oeste do Paraná (HUOP. Conta com corpo docente composto por 1.229 docentes e 1.267 agentes universitários. Para o problema desta pesquisa, tem-se: é possível a criação de um Balanced Scorecard para uma Universidade Pública? Para o tratamento dos dados, utilizou-se a análise de conteúdo, apoiado pelo software ATLAS.ti. Ao final do trabalho, propôs-se um total de 5 perspectivas: Educacional, Financeira, Sociedade, Aprendizado e Crescimento e Processos Internos, distribuídos nestas perspectivas, 16 objetivos estratégicos e 30 indicadores estratégicos. A contribuição deste artigo consiste em apresentar as especificidades não abordadas no modelo tradicional, proposto por Kaplan e Norton, a uma instituição que se apresenta nas características de instituição pública e voltada para o ensino superior.

  20. Longitudinal distribution and seasonality of macroalgae in a subtropical stream impacted by organic pollution Distribuição longitudinal e sazonalidade de macroalgas em um riacho subtropical impactado por poluição orgânica

    Directory of Open Access Journals (Sweden)

    Cleto Kaveski Peres

    2010-06-01

    Full Text Available AIM: This study was carried out to assess longitudinal and temporal distribution and the effects of organic pollution on macroalgal communities in a subtropical stream; METHODS: The occurrence and percent cover of stream macroalgae were investigated during seasonal period in four sampling sites along the course of the Cascavel Stream, in Paraná State, subtropical area of Brazil. Sampling sites were randomly chosen; however, their location in relation to urban organic pollution sources was taken into consideration. Besides, several stream variables usually related to organic pollution in aquatic ecosystems were measured; RESULTS: Eleven macroalgal taxa were found during the samplings. Chlorophyta was the prevailing division (4 species or 36.3% of the total richness, followed by Cyanophyta and Rhodophyta (3 or 27.3% and Heterokontophyta (1 or 9.1%. Analysis of variance (ANOVA showed no significant differences in species diversity, percent cover and diversity index among seasons or the sampling sites. In addition, species diversity was not correlated with any environmental variable in particular. On the other hand, the relative composition of the stream macroalgal communities showed wide spatial and temporal variation. Thus, Chlorophyta had higher values of richness and percent cover in sampling site with higher levels of organic pollution, while Rhodophyta was never found in this segment. Taking seasonality into consideration, Chlorophyta had higher values of percent cover in winter, but Rhodophyta and Cyanophyta in spring and fall, respectively; CONCLUSIONS: In general terms, in this study macroalgal community structures were slightly affected by organic pollution, mainly by increase in richness and percent cover of Chlorophyta associated with the absence of Rhodophyta in the most polluted sampling site. The analysis of the richness throughout the stretch of the stream showed that the highest values were found in the intermediate part, being

  1. Geoprocessing applied to the phytosociological survey in permanent plots El geo-procesamiento aplicado al levantamiento fito-sociológico en parcelas permanentes Geoprocessamento aplicado ao levantamento fitossociológico em parcelas permanentes

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    Luiz Gilberto Bertotti

    2009-12-01

    Full Text Available The goal with this work was to create a georeferenced data bank to follow the phytosociological changes of types of trees along the time and its geographic distribution in the field of study. To the phytosociological survey permanent parcels were plotted in the field of study along the floodplain of the rivers Carro Quebrado and Cascavel, both located in the Campus of the Universidade Estadual do Centro Oeste (UNICENTRO – CEDETEG, in Guarapuava city, Parana state. In each part the attributes analyzed were: sociologic position (SP; class of crown (CC; characteristics of trees (CT; quality conditions of the trunk (QCT; conditions (Cs. These parts were put into spaces with use of the Global Positioning System (GPS and the data were sent to the SPRING software with the intention to build a cadastral data bank to follow and analyze the development of the local vegetation. The creation of this data bank allowed following the development of trees in a time scale, showing the evolution of the part of the forest studied.Este trabajo en su objeto pretende crear un banco de informaciones geo-referenciado para acompañar los cambios fitosociológicos de los indivíduos arbóreos a lo largo del tiempo y su distribuccíon geográfica en el área del estudio. Demarcamos parcelas permanentes a lo largo de la planicie de inundación de los rios “Carro  Quebrado” y Cascavel” que están localizados en el campus de la Universidad Estadual Del Centro Oeste del Estado del Paraná, municipalidad de Guarapuava ( UNICENTRO –CEDETEG.En cada parcela de tierra separada evaluamos distintos atributos: PS: localización sociológica; CC: tipo de copa; CA: características de los árboles; CCT: condiciones de calidad del tronco; Cs: otras condiciones.  Estas parcelas fueron especializadas a partir de la utilización del Sistema de  Pocsicionamiento Global (GPS y todos los datos fuero exportados para el sftware SPRING a fin de construir el banco de informaciones

  2. Gênero e distribuição espacial da população no oeste do Paraná

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    Lucir Reinaldo Alves

    2013-04-01

    Full Text Available Neste artigo analisa-se o perfil da localização e distribuição espacial da população masculina e feminina no oeste do Paraná. Os dados de população coletados pelo Instituto Brasileiro de Geografia e Estatística (IBGE foram desagrupados em quatro variáveis: população urbana feminina, população rural feminina, população urbana masculina e população rural masculina, distribuídos nos cinquenta municípios que compõem a Região. O período estudado abrangeu os anos de 2000 e 2010. Os dados foram analisados a partir de alguns indicadores de localização consagrados pela análise regional. Os resultados mostraram que Cascavel, Foz do Iguaçu e Toledo possuem uma população urbana masculina e feminina mais numerosa que os demais municípios. Já os demais municípios estão consolidando suas economias, seja na transformação agroalimentar, seja na prestação de serviços, fato que se reflete em uma maior participação da população urbana, que cresce mais rapidamente em relação a população rural.Palavras-chave | Distribuição espacial da população; gênero; oeste do Paraná; região.Código JEL | J16; R12; 018. GENDER AND SPATIAL DISTRIBUTION OF POPULATION IN WESTERN PARANÁAbstractThis paper analyzes the profile of the location and spatial distribution of male and female population inWestern Paraná. Population data collected by the Brazilian Institute of Geography and Statistics (IBGE were grouped into four variables: urban female population, rural female population, urban male population and rural male population, distributed in the fifty municipalities that comprise the region. The periods studied are the years of 2000 and 2010. Data were analyzed from some location indicators established by the regional analysis. The results showed that Cascavel, Foz do Iguaçu andToledohave a male and female urban population larger than the other municipalities. The other municipalities are consolidating their economies, whether

  3. Modulation of the pharmacological effects of enzymatically-active PLA2 by BTL-2, an isolectin isolated from the Bryothamnion triquetrum red alga

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    Nagano Celso S

    2008-06-01

    Full Text Available Abstract Background An interaction between lectins from marine algae and PLA2 from rattlesnake was suggested some years ago. We, herein, studied the effects elicited by a small isolectin (BTL-2, isolated from Bryothamnion triquetrum, on the pharmacological and biological activities of a PLA2 isolated from rattlesnake venom (Crotalus durissus cascavella, to better understand the enzymatic and pharmacological mechanisms of the PLA2 and its complex. Results This PLA2 consisted of 122 amino acids (approximate molecular mass of 14 kDa, its pI was estimated to be 8.3, and its amino acid sequence shared a high degree of similarity with that of other neurotoxic and enzymatically-active PLA2s. BTL-2 had a molecular mass estimated in approximately 9 kDa and was characterized as a basic protein. In addition, BTL-2 did not exhibit any enzymatic activity. The PLA2 and BTL-2 formed a stable heterodimer with a molecular mass of approximately 24–26 kDa, estimated by molecular exclusion HPLC. In the presence of BTL-2, we observed a significant increase in PLA2 activity, 23% higher than that of PLA2 alone. BTL-2 demonstrated an inhibition of 98% in the growth of the Gram-positive bacterial strain, Clavibacter michiganensis michiganensis (Cmm, but only 9.8% inhibition of the Gram-negative bacterial strain, Xanthomonas axonopodis pv passiflorae (Xap. PLA2 decreased bacterial growth by 27.3% and 98.5% for Xap and Cmm, respectively, while incubating these two proteins with PLA2-BTL-2 inhibited their growths by 36.2% for Xap and 98.5% for Cmm. PLA2 significantly induced platelet aggregation in washed platelets, whereas BTL-2 did not induce significant platelet aggregation in any assay. However, BTL-2 significantly inhibited platelet aggregation induced by PLA2. In addition, PLA2 exhibited strong oedematogenic activity, which was decreased in the presence of BTL-2. BTL-2 alone did not induce oedema and did not decrease or abolish the oedema induced by the 48

  4. Modulation of the pharmacological effects of enzymatically-active PLA2 by BTL-2, an isolectin isolated from the Bryothamnion triquetrum red alga

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    Oliveira, Simone CB; Fonseca, Fabiana V; Antunes, Edson; Camargo, Enilton A; Morganti, Rafael P; Aparício, Ricardo; Toyama, Daniela O; Beriam, Luís OS; Nunes, Eudismar V; Cavada, Benildo S; Nagano, Celso S; Sampaio, Alexandre H; Nascimento, Kyria S; Toyama, Marcos H

    2008-01-01

    Background An interaction between lectins from marine algae and PLA2 from rattlesnake was suggested some years ago. We, herein, studied the effects elicited by a small isolectin (BTL-2), isolated from Bryothamnion triquetrum, on the pharmacological and biological activities of a PLA2 isolated from rattlesnake venom (Crotalus durissus cascavella), to better understand the enzymatic and pharmacological mechanisms of the PLA2 and its complex. Results This PLA2 consisted of 122 amino acids (approximate molecular mass of 14 kDa), its pI was estimated to be 8.3, and its amino acid sequence shared a high degree of similarity with that of other neurotoxic and enzymatically-active PLA2s. BTL-2 had a molecular mass estimated in approximately 9 kDa and was characterized as a basic protein. In addition, BTL-2 did not exhibit any enzymatic activity. The PLA2 and BTL-2 formed a stable heterodimer with a molecular mass of approximately 24–26 kDa, estimated by molecular exclusion HPLC. In the presence of BTL-2, we observed a significant increase in PLA2 activity, 23% higher than that of PLA2 alone. BTL-2 demonstrated an inhibition of 98% in the growth of the Gram-positive bacterial strain, Clavibacter michiganensis michiganensis (Cmm), but only 9.8% inhibition of the Gram-negative bacterial strain, Xanthomonas axonopodis pv passiflorae (Xap). PLA2 decreased bacterial growth by 27.3% and 98.5% for Xap and Cmm, respectively, while incubating these two proteins with PLA2-BTL-2 inhibited their growths by 36.2% for Xap and 98.5% for Cmm. PLA2 significantly induced platelet aggregation in washed platelets, whereas BTL-2 did not induce significant platelet aggregation in any assay. However, BTL-2 significantly inhibited platelet aggregation induced by PLA2. In addition, PLA2 exhibited strong oedematogenic activity, which was decreased in the presence of BTL-2. BTL-2 alone did not induce oedema and did not decrease or abolish the oedema induced by the 48/80 compound. Conclusion The

  5. Snake venomics and antivenomics of Bothrops colombiensis, a medically important pitviper of the Bothrops atrox-asper complex endemic to Venezuela: Contributing to its taxonomy and snakebite management.

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    Calvete, Juan J; Borges, Adolfo; Segura, Alvaro; Flores-Díaz, Marietta; Alape-Girón, Alberto; Gutiérrez, José María; Diez, Nardy; De Sousa, Leonardo; Kiriakos, Demetrio; Sánchez, Eladio; Faks, José G; Escolano, José; Sanz, Libia

    2009-03-06

    The taxonomic status of the medically important pitviper of the Bothrops atrox-asper complex endemic to Venezuela, which has been classified as Bothrops colombiensis, remains incertae cedis. To help resolving this question, the venom proteome of B. colombiensis was characterized by reverse-phase HPLC fractionation followed by analysis of each chromatographic fraction by SDS-PAGE, N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of tryptic peptides. The venom contained proteins belonging to 8 types of families. PI Zn(2+)-metalloproteinases and K49 PLA(2) molecules comprise over 65% of the venom proteins. Other venom protein families comprised PIII Zn(2+)-metalloproteinases (11.3%), D49 PLA(2)s (10.2%), l-amino acid oxidase (5.7%), the medium-sized disintegrin colombistatin (5.6%), serine proteinases (1%), bradykinin-potentiating peptides (0.8%), a DC-fragment (0.5%), and a CRISP protein (0.1%). A comparison of the venom proteomes of B. colombiensis and B. atrox did not support the suggested synonymy between these two species. The closest homologues to B. colombiensis venom proteins appeared to be toxins from B. asper. A rough estimation of the similarity between the venoms of B. colombiensis and B. asper indicated that these species share approximately 65-70% of their venom proteomes. The close kinship of B. colombiensis and B. asper points at the ancestor of B. colombiensis as the founding Central American B. asper ancestor. This finding may be relevant for reconstructing the natural history and cladogenesis of Bothrops. Further, the virtually indistinguishable immunological crossreactivity of a Venezuelan ABC antiserum (raised against a mixture of B. colombiensis and Crotalus durissus cumanensis venoms) and the Costa Rican ICP polyvalent antivenom (generated against a mixture of B. asper, Crotalus simus, and Lachesis stenophrys venoms) towards the venoms of B. colombiensis and B. asper, supports this

  6. Quadro clínico-patológico do envenenamento crotálico experimental em bubalinos comparado com o de bovinos

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    José Diomedes Barbosa

    2011-11-01

    Full Text Available O estudo teve por objetivo verificar a sensibilidade dos bubalinos à peçonha de Crotalus durissus terriiicus, estudar o quadro clínico-patológico e laboratorial nessa espécie e estabelecer comparações com o verificado em bovinos. A inoculação do veneno liofilizado de Crotalus durissus terriiicus, diluído em 1ml de solução fisiológica, foi feita na região da articulação úmero-rádio-ulnar, por via subcutânea, em três bubalinos (doses de 0,015; 0,03; e 0,066mg/kg e em dois bovinos (doses de 0,03 e 0,066mg/kg. O bubalino que recebeu a dose de 0,03mg/ kg apresentou sinais clínicos graves recuperou-se seis dias após, e o bovino que recebeu a mesma dose morreu com evolução de 22h56min. A dose de 0,066mg/kg causou a morte tanto do bovino quanto do bubalino, com evolução clínica de 4h23min e 8h12min, respectivamente. O bubalino que recebeu a dose de 0,015mg/kg, recuperou-se com evolução de 48 horas. Os sinais clínicos tiveram início dentro de 3h58min no bubalino que morreu, e nos bubalinos que adoeceram mas se recuperaram, dentro de 17h25min e 24h00min após a inoculação do veneno. Nos dois bovinos que morreram (com doses de 0,03 e 0,066mg/kg, os primeiros sinais clínicos foram observados 6h10min e 6h31min após a inoculação do veneno. A inoculação do veneno produziu nos búfalos e bovinos um quadro nervoso de paralisia flácida. Os principais sinais observados tanto nos búfalos quanto nos bovinos, foram discreto aumento de volume no local da inoculação, dificuldade respiratória caracterizada por respiração predominantemente abdominal, apatia, sialorreia, dificuldade para se levantar quando estimulados, evolução para decúbito esternal permanente, seguido de decúbito lateral e movimentos de pedalagem, e diminuição dos reflexos relacionados aos pares de nervos cranianos. Nos bubalinos adicionalmente foi observado aumento da base de sustentação, arrastar das pinças dos membros posteriores, marcha lenta e

  7. Milk line as an indicator of the harvesting time of three hybrid seeds of corn (Zea mays L.

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    Célio Trzeciak dos Santos

    2005-03-01

    Full Text Available The experiment was carried out to evaluate the time for harvesting seeds of three hybrids of corn (Zea mays L. at their best physiological quality and the use of the milk line as an indicator of the physiological seed maturity. Single hybrids CD 1723 and CD 5501 and double hybrid OC 705 seeds were collected for 59 days, every four days, starting 23 days after female flowering. Seed dry weight, moisture content, germination, vigour (cold and accelerated aging tests, black layer formation and seed milk line development were analysed. The harvesting time started 47 days after female flowering with high physiological quality of the seeds identified by a joint analysis of those seven characteristics of them. The milk line at stage 4 proved to be the best indicator of the time to harvest corn seeds for maximum physiological quality, because different stages of milk line development in corn seeds could be easily identified in the filed without any special equipment.O experimento foi conduzido durante o ano agrícola de 1996/97 em área experimental da Cooperativa de Desenvolvimento Econômico e Tecnológico (Coodetec, em Cascavel, Paraná, com o objetivo de avaliar o efeito da época de colheita de três híbridos de milho (Zea mays L. na qualidade fisiológica das sementes e o uso da linha de solidificação do endosperma como indicativo da maturidade fisiológica das sementes. O plantio foi realizado em 30 de setembro de 1996. Amostras de sementes dos híbridos simples CD 1723 e CD 5501, bem como do híbrido duplo OC 705, foram colhidas em intervalos de quatro dias, durante 59 dias, iniciando-se a colheita no vigésimo terceiro dia após o florescimento feminino. As características avaliadas nas sementes foram o acúmulo de matéria seca, o conteúdo de umidade, a germinação, o vigor (pelos testes de frio e de envelhecimento acelerado, a formação da camada preta e o desenvolvimento da linha de solidificação do endosperma. A colheita dos tr

  8. Animal-based medicines used in ethnoveterinary practices in the semi-arid region of Northeastern Brazil

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    Wedson M.S. Souto

    2012-09-01

    principais espécies citadas foram: o carneiro - Ovis aries (UV=0,89, a raposa - Cerdocyon thous (UV=0,79, o camaleão - Iguana iguana (UV=0,79 e a cascavel - Caudisona durissa (Linnaeus, 1758 (UV=0,74. Os tratamentos mais freqüentemente citados relacionaram-se a doenças inflamatórias, dermatológicas e desordens obstetrícias. Semelhante a outros estudos, a etnoveterinária local estabelece conexões com a etnomedicina para humanos. Os resultados sugerem que similaridades no repertório de recursos medicinais escolhidos pelos moradores locais refletem a acessibilidade/disponbilidade dos recursos. Nosso resultados ajudam a preservar o conhecimento etnoveterinário, o qual é importante para aumentar nossa compreensão das relações entre seres humanos, a sociedades e a natureza, e também elaborar estratégias mais efetivas para conservar os recursos naturais. Outros estudos para validação científica da eficácia e efeitos colaterais destes produtos zooterapêuticos são necessários antes que possam ser recomendados ou não para o uso.

  9. CONSUMO DE LEITE DE VACA E DERIVADOS ENTRE AGRICULTORES DA REGIÃO OESTE DO PARANÁ

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    Heloísa TOMBINI

    2012-04-01

    Full Text Available O leite é a principal fonte de cálcio na alimentação humana, cuja importância está relacionada às suas funções no organismo humano, pois contribui para a formação do tecido ósseo, promove o crescimento, regula o sistema nervoso e aumenta a resistência a infecções. Seus principais nutrientes são: proteínas de alta qualidade, gorduras, carboidratos, além de ser rico em vitaminas e minerais. O objetivo deste estudo foi identificar o consumo de leite de vaca e derivados na população rural residente na região oeste do Paraná. O estudo transversal descritivo foi realizado durante o 20º Show Rural Coopavel, no ano de 2009, em Cascavel, Paraná, com agricultores moradores das áreas urbana e rural dessa região. Para verificar os hábitos alimentares foi aplicado o teste “Como está a sua Alimentação?” validado pelo Ministério da Saúde, cuja classificação segue as porções diárias de leite e derivados recomendadas pela pirâmide alimentar para a população adulta. Dos 372 agricultores que participaram do teste, 193 responderam sobre o consumo de leite, e destes, 160 afirmaram consumir leite e derivados, dos quais 82,5% apresentaram consumo maior ou igual à recomendação e 17,5% apresentaram consumo menor que a recomendação. Em relação à adequação de consumo, 82,7% das mulheres e 82,2% dos homens consomem leite e derivados dentro das recomendações estabelecidas pela pirâmide alimentar, assim como 81,6% dos adultos e 89,5% dos idosos. Segundo as classes econômicas, o consumo adequado de leite e derivados foi verificado em 85,7% na classe A, 90,2% na classe B, 77,5% na classe C e 83,3% na classe D+E, não havendo diferença significativa entre estas variáveis. O tipo de leite mais consumido pelos agricultores foi o leite integral (86,1% e apenas 13,9% relataram ingerir leite desnatado. De acordo com o local de domicílio 20% residia na zona urbana e 80% no meio rural, sendo que dos residentes na cidade, 90

  10. Revascularização do miocárdio em pacientes após a oitava década de vida

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    ALMEIDA Rui Manuel Sequeira de

    2002-01-01

    Full Text Available OBJETIVO: Avaliar a morbi-mortalidade hospitalar e a qualidade de vida de pacientes, acima de 70 anos de vida, submetidos à operação de revascularização do miocárdio (CRM. CASUÍSTICA E MÉTODOS: No período de julho de 1992 a fevereiro de 2000, foram realizadas 507 CRM, no Serviço de Cirurgia Cardiovascular do Instituto de Moléstias Cardiovasculares de Cascavel. Em 70 destes casos os pacientes tinham idade igual ou superior a 70 anos. Neste grupo predominou o sexo masculino, em 57% dos casos, e a idade média foi de 72,9 anos (70-85 anos. Vinte e seis pacientes apresentavam hipertensão arterial sistêmica, 25 doença pulmonar obstrutiva crônica importante, 17 diabete melito e 8 insuficiência renal crônica, no pré-operatório. Trinta e sete pacientes apresentavam infarto agudo do miocárdio (IAM pré-operatório, sete haviam sido submetidos à angioplastia transluminal percutânea, sete apresentavam lesão de tronco de artéria coronária esquerda e um havia sido submetido à CRM anteriormente. Foram realizados 2,8 enxertos/ paciente, sendo usados condutos arteriais em 53% dos casos. Foi realizada endarterectomia em sete artérias, aneurismectomia de ventrículo esquerdo em sete pacientes e ventriculectomia parcial esquerda em um. A operação foi realizada em caráter de emergência em nove casos. Houve necessidade de contrapulsação aórtica em quatro pacientes. RESULTADOS: O tempo médio de permanência na UTI e no hospital foi de 4 (1-24 e 12,2 (3-34 dias, respectivamente. A mortalidade hospitalar geral foi de 7,1%. Quando analisada por subgrupos, a mortalidade dos pacientes de 70 a 74 anos (57 casos foi de 5,3%, e a dos últimos 35 casos de 2,8%. No pós-operatório imediato, as complicações mais freqüentes foram: insuficiência respiratória (10, arritmia atrial (7, alteração de conduta (6, infecção pulmonar (6, embolia pulmonar (5, síndrome de baixo débito (4, IAM (3, AVC (3, insuficiência renal aguda (4 e

  11. ESTIMATIVA DA EROSIVIDADE DE CHUVAS NO ESTADO DO PARANÁ PELO MÉTODO DA PLUVIOMETRIA: ATUALIZAÇÃO COM DADOS DE 1986 A 2008

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    Paulo Cesar Waltrick

    2015-02-01

    Full Text Available Três estimativas de erosividade de chuvas no Estado do Paraná foram feitas nas décadas de 1980-90, mas nenhuma outra depois. Os objetivos deste trabalho foram atualizar, numa quarta estimativa, as informações de erosividade anual das chuvas para o Paraná, com dados pluviométricos de 1986 a 2008; e gerar informações de erosividade mensal para identificar a distribuição temporal da erosividade. Foram utilizados os métodos pluviográfico e pluviométrico de determinação de erosividade. O primeiro serviu para revalidar equações prévias de correlação entre erosividade por pluviografia e por pluviometria, isso feito em três localidades; já o segundo, para calcular o coeficiente de chuva em 114 localidades, utilizando-se dados de precipitação média mensal e anual, e então a erosividade, com as equações de correlação revalidadas. Mapas de erosividade anual e mensal foram gerados por interpolações geoestatísticas. A erosividade variou entre 5.449 e 12.581 MJ mm ha-1 h-1 ano-1 e aumentou no sentido oeste, a partir da região metropolitana de Curitiba; e no sentido sul, a partir do noroeste paranaense. Isso resultou em maiores erosividades nas regiões centro-sul (Guarapuava, centro-ocidental (Campo Mourão, oeste (Cascavel e, principalmente, sudoeste (Pato Branco. Também houve incremento de erosividade a partir da região metropolitana de Curitiba, no sentido leste, indicando o litoral como outra região crítica em termos de erosividade. A maior erosividade mensal ocorreu em janeiro (média estadual de 1394 MJ mm ha-1 h-1 mês-1, seguida por tendência de diminuição até agosto (331 MJ mm ha-1 h-1 mês-1, quando então tornou a aumentar. Porém, ocorrem dois importantes repiques de erosividade: um em maio e outro em outubro (843 e 1.173 MJ mm ha-1 h-1 mês-1, respectivamente, principalmente nas regiões sudoeste e oeste. Esses repiques coincidem com períodos de implantação de culturas de inverno ou de verão nessas

  12. Aspectos clínico-patológicos da intoxicação experimental pelas sementes de Crotalaria mucronata (Fabaceae em bovinos Experimental poisoning by Crotalaria mucronata (Fabaceae seeds in cattle

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    Murilo R. Boghossian

    2007-04-01

    Full Text Available Sementes moídas de Crotalaria mucronata Desv. (=Crotalaria striata DC., Crotalaria pallida Ait., Crotalaria saltiana, com nomes populares de "xique-xique" ou "guizo-de-cascavel", foram administradas por via oral a 9 bovinos. As doses diárias de 1g/kg, 2g/kg, 3g/kg cada uma a um bovino, e 5g/kg em dois de três bovinos, dadas durante 61 a 63 dias, não causaram intoxicação. A dose de 5g/kg em um bovino, 7,5g/kg em dois bovinos e 10g/kg em um bovino, dadas durante 47-61 dias, causaram sintomas entre 47 e 80 dias após o início da administração e a morte entre 3 horas e 5 dias após o início dos sintomas. Os principais sintomas foram pulso venoso positivo da veia jugular, respiração abdominal, taquicardia, inapetência, fezes ressequidas, edema sub-mandibular e fraqueza. Os achados de necropsia foram palidez pulmonar, hidropericárdio, hidrotórax, hidroperitôneo, edema de mesentério, aumento da consistência hepática, alterações de cor do fígado, dilatação de ventrículo cardíaco direito e edema da parede ruminal. As principais alterações histológicas concentraram-se nos pulmões, sob forma de espessamento das paredes alveolares e da parede das arteríolas com diminuição da luz e fibrose periarteriolar; havia também lesões hepáticas e cardíacas de menor importância. Pode-se concluir que as principais lesões causadas pela ingestão das sementes de C. mucronata durante períodos prolongados, devem-se à dificuldade de passagem do sangue pelos vasos pulmonares em função da hipertensão arterial decorrente de fibrose e espessamento arteriolar determinada pela ação pneumotóxica da planta.Experiments were performed to define the clinical and pathological picture of prolonged administration of the seeds of Crotalaria mucronata Desv. to cattle, in order to obtain additional information about this toxicosis. The ground seeds were administered orally to 9 bovines. Doses of 1g/kg/day, 2g/kg/day, 3g/kg/day each in one bovine

  13. Contribuição para o conhecimento da intoxicação pelo veneno dos "escorpiões"

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    Octavio de Magalhães

    1928-01-01

    0,gr.000.106.15. Pour 1.149 T. serrulatus, la moyenne pour une piqûre est.......0,gr.000.246.30. La quantité a varié, selon les individus, de 0,gr.000.035.71, à 0,gr.000.436.01 de venin pur, pour une piqûre. D'après ce qui vient d'étre dit, on peut voir combien la quantité de venin éjaculé varie, chaque fois, chez les scorpions. L'unité-piqûre ne peut done pas ètre utiliseé pour des expériences dèlicates. Le mieux est de se servir de venin pur, et c'est ce que nous avons fait pour les expériences minutieuses. Quand on n'en possède pas, on peut établir pour chaque série des expériences à tenter-la dose minima mortelle en poids (grammes et fractions de vésicules. D'après les bases ici consignées, et avec une trés petite erreur, on peut calculer la quantité de venin pur de cette dóse. Ce calcul est d'ailleurs dispensable. On peut s'en rapporter simplement au poids sec des vésicules totales et dire que la D. m. m. est de tant de milligr. secs. Comme le venin se conserve mal dans les vésicules, il faut, dans ce procédé, doser la D. m. m. toutes les fois que l'on veut procéder á une sériê d'expériences. Le venin desséché rappelle, d'après le temps de conservations au Laboratoire, celui de Crotatus terrificus et celui des Lachesis (quand il est vieux. Il est retenu au passage en partie, par les bougies Berkfeld et Chamberland. La conservation en état de dessication est la meilleure. Ainsi gardé, à l'abri de la lumierè, aux approches de 0,gr., pendant 8 mois, il perd à peine 1,2 à 1,4 de sa valeur primitive. L'echauffement à 100 gr. trouble une dissolution de venin dans l'eau distilleé; sans atteindre toutefois son pouvoir toxique, quand on l'injecte par la voie intra-cérébrale. Nous avons fait l'experience par 11 voies diverses. Sur des animaux sensibles, nous n'avons pas obtenu de phénomènes toxiques, apparemment, par les voies suivantes: 1 buccale; 2 gastrique; 3 rectale; 4 chambre oculaire antérieure; 5 cornéenne; 6