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Sample records for durch rhodopseudomonas palustris

  1. Sulfoacetate generated by Rhodopseudomonas palustris from taurine.

    Science.gov (United States)

    Denger, Karin; Weinitschke, Sonja; Hollemeyer, Klaus; Cook, Alasdair M

    2004-10-01

    Genes thought to encode (a) the regulator of taurine catabolism under carbon-limiting or nitrogen-limiting conditions and (b) taurine dehydrogenase were found in the genome of Rhodopseudomonas palustris. The organism utilized taurine quantitatively as a sole source of nitrogen (but not of carbon) for aerobic and photoheterotrophic growth. No sulfate was released, and the C-sulfonate bond was recovered stoichiometrically as sulfoacetate, which was identified by mass spectrometry. An inducible sulfoacetaldehyde dehydrogenase was detected. R. palustris thus contains a pathway to generate a natural product that was previously believed to be formed solely from sulfoquinovose.

  2. Sulfoacetate generated by Rhodopseudomonas palustris from taurine

    OpenAIRE

    Denger, Karin; Weinitschke, Sonja; Hollemeyer, Klaus; Cook, Alasdair M.

    2004-01-01

    Genes thought to encode (a) the regulator of taurine catabolism under carbon-limiting or nitrogen-limiting conditions and (b) taurine dehydrogenase were found in the genome of Rhodopseudomonas palustris. The organism utilized taurine quantitatively as a sole source of nitrogen (but not of carbon) for aerobic and photoheterotrophic growth. No sulfate was released, and the C-sulfonate bond was recovered stoichiometrically as sulfoacetate, which was identified by mass spectrometry. An inducible ...

  3. Essential Genome of the Metabolically Versatile Alphaproteobacterium Rhodopseudomonas palustris

    Science.gov (United States)

    Pechter, Kieran B.; Gallagher, Larry; Pyles, Harley; Manoil, Colin S.

    2015-01-01

    ABSTRACT Rhodopseudomonas palustris is an alphaproteobacterium that has served as a model organism for studies of photophosphorylation, regulation of nitrogen fixation, production of hydrogen as a biofuel, and anaerobic degradation of aromatic compounds. This bacterium is able to transition between anaerobic photoautotrophic growth, anaerobic photoheterotrophic growth, and aerobic heterotrophic growth. As a starting point to explore the genetic basis for the metabolic versatility of R. palustris, we used transposon mutagenesis and Tn-seq to identify 552 genes as essential for viability in cells growing aerobically on semirich medium. Of these, 323 have essential gene homologs in the alphaproteobacterium Caulobacter crescentus, and 187 have essential gene homologs in Escherichia coli. There were 24 R. palustris genes that were essential for viability under aerobic growth conditions that have low sequence identity but are likely to be functionally homologous to essential E. coli genes. As expected, certain functional categories of essential genes were highly conserved among the three organisms, including translation, ribosome structure and biogenesis, secretion, and lipid metabolism. R. palustris cells divide by budding in which a sessile cell gives rise to a motile swarmer cell. Conserved cell cycle genes required for this developmental process were essential in both C. crescentus and R. palustris. Our results suggest that despite vast differences in lifestyles, members of the alphaproteobacteria have a common set of essential genes that is specific to this group and distinct from that of gammaproteobacteria like E. coli. IMPORTANCE Essential genes in bacteria and other organisms are those absolutely required for viability. Rhodopseudomonas palustris has served as a model organism for studies of anaerobic aromatic compound degradation, hydrogen gas production, nitrogen fixation, and photosynthesis. We used the technique of Tn-seq to determine the essential genes of

  4. Metabolic engineering of Rhodopseudomonas palustris for squalene production.

    Science.gov (United States)

    Xu, Wen; Chai, Changbin; Shao, Lingqiao; Yao, Jia; Wang, Yang

    2016-05-01

    Squalene is a strong antioxidant used extensively in the food, cosmetic and medicine industries. Rhodopseudomonas palustris TIE-1 was used as the host because of its ability to grow photosynthetically using solar energy and carbon dioxide from atmosphere. The deletion of the shc gene resulted in a squalene production of 3.8 mg/g DCW, which was 27-times higher than that in the wild type strain. For constructing a substrate channel to elevate the conversion efficiency, we tried to fuse crtE gene with hpnD gene. By fusing the two genes, squalene content was increased to 12.6 mg/g DCW, which was 27.4 % higher than that resulted from the co-expression method. At last, the titer of squalene reached 15.8 mg/g DCW by co-expressing the dxs gene, corresponding to 112-fold increase relative to that for wild-type strain. This study provided novel strategies for improving squalene yield and demonstrated the potential of producing squalene by Rhodopseudomonas palustris.

  5. Insights into arsenic multi-operons expression and resistance mechanisms in Rhodopseudomonas palustris CGA009

    OpenAIRE

    Zhao, Chungui; Zhang, Yi; Chan, Zhuhua; Chen, Shicheng; YANG, SUPING

    2015-01-01

    Arsenic (As) is widespread in the environment and causes numerous health problems. Rhodopseudomonas palustris has been regarded as a good model organism for studying arsenic detoxification since it was first demonstrated to methylate environmental arsenic by conversion to soluble or gaseous methylated species. However, the detailed arsenic resistance mechanisms remain unknown though there are at least three arsenic-resistance operons (ars1, ars2, and ars3) in R. palustris. In this study, we i...

  6. Insights into arsenic multi-operons expression and arsenic resistance mechanisms in Rhodopseudomonas palustris CGA009

    OpenAIRE

    Chungui eZhao; Yi eZhang; Zhuhua eChan; Shicheng eChen; Suping eYang

    2015-01-01

    Arsenic (As) is widespread in the environment and causes numerous health problems. Rhodopseudomonas palustris has been regarded as a good model organism for studying arsenic detoxification since it was first demonstrated to methylate environmental arsenic by conversion to soluble or gaseous methylated species. However, the detailed arsenic resistance mechanisms remain unknown though there are at least three arsenic-resistance operons (ars1, ars2 and ars3) in R. palustris. In this study, we i...

  7. Influence of growth rate and starvation on fluorescent in situ hybridization of Rhodopseudomonas palustris

    NARCIS (Netherlands)

    Oda, Y; Slagman, SJ; Meijer, WG; Forney, LJ; Gottschal, JC

    In situ hybridization with a fluorescently labeled 16S rRNA-targeted probe was examined using Rhodopseudomonas palustris as a model organism, which had been grown at different rates and under different conditions of growth and starvation. The specific growth rate did not affect the percentage of

  8. Growth performance and meat quality of broiler chickens supplemented with Rhodopseudomonas palustris in drinking water.

    Science.gov (United States)

    Xu, Q Q; Yan, H; Liu, X L; Lv, L; Yin, C H; Wang, P

    2014-01-01

    1. The effect of the bacterium, Rhodopseudomonas palustris, on the growth performance and meat quality of broiler chickens was investigated. 2. A total of 900-d-old Arbor Acres broilers were allocated to three experimental treatments for 6 weeks. Chicks were administered with R. palustris in drinking water as follows: (i) control group without R. palustris; (ii) treatment 1 (R1) with R. palustris of 8 × 10(9) cells per chick per day in drinking water; (iii) treatment 2 (R2) with R. palustris of 1.6 × 10(10) cells per chick per day in drinking water. 3. The results showed that, compared with that of control, both groups of R. palustris treatment increased daily weight gain and improved feed conversion ratio of broiler chickens significantly during the whole growing period of 6 weeks. 4. Both total and glutamic acid contents of chicken breast fillet in R. palustris treatment R2 were higher, while the fat content was lower, than those of the control group. Furthermore, R. palustris treatments also improved sensory attributes of chicken breast fillet. 5. As a probiotic providing rich nutrients and biological active substances, R. palustris administration in drinking water displayed a growth promoting effect and improved meat quality of broiler chickens.

  9. Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris

    Energy Technology Data Exchange (ETDEWEB)

    Larimer, Frank W [ORNL; Chain, Patrick S. G. [Lawrence Livermore National Laboratory (LLNL); Hauser, Loren John [ORNL; Lamerdin, Jane [Lawrence Livermore National Laboratory (LLNL); Malfatti, Stephanie [Lawrence Livermore National Laboratory (LLNL); Do, Long [Lawrence Livermore National Laboratory (LLNL); Land, Miriam L [ORNL; Pelletier, Dale A [ORNL; Beatty, Thomas [University of British Columbia, Vancouver; Lang, Andrew S. [University of British Columbia, Vancouver; Tabita, F Robert [Ohio State University, The, Columbus; Gibson, Janet L. [University of Iowa; Hanson, Thomas E. [Ohio State University, The, Columbus; Bobst, Cedric [Ohio State University, The, Columbus; Torres y Torres, Janelle L. [University of Iowa; Peres, Caroline [University of Iowa; Harrison, Faith H. [University of Iowa; Gibson, Jane [University of Iowa; Harwood, Caroline S [University of Washington, Seattle

    2004-01-01

    Rhodopseudomonas palustris is among the most metabolically versatile bacteria known. It uses light, inorganic compounds, or organic compounds, for energy. It acquires carbon from many types of green plant-derived compounds or by carbon dioxide fixation, and it fixes nitrogen. Here we describe the genome sequence of R. palustris, which consists of a 5,459,213-base-pair (bp) circular chromosome with 4,836 predicted genes and a plasmid of 8,427 bp. The sequence reveals genes that confer a remarkably large number of options within a given type of metabolism, including three nitrogenases, five benzene ring cleavage pathways and four light harvesting 2 systems. R. palustris encodes 63 signal transduction histidine kinases and 79 response regulator receiver domains. Almost 15% of the genome is devoted to transport. This genome sequence is a starting point to use R. palustris as a model to explore how organisms integrate metabolic modules in response to environmental perturbations.

  10. Experimental and Computational Investigation of Biofilm Formation by Rhodopseudomonas palustris Growth under Two Metabolic Modes.

    Directory of Open Access Journals (Sweden)

    Chase Kernan

    Full Text Available We examined biofilms formed by the metabolically versatile bacterium Rhodopseudomonas palustris grown via different metabolic modes. R. palustris was grown in flow cell chambers with identical medium conditions either in the presence or absence of light and oxygen. In the absence of oxygen and the presence of light, R. palustris grew and formed biofilms photoheterotrophically, and in the presence of oxygen and the absence of light, R. palustris grew and formed biofilms heterotrophically. We used confocal laser scanning microscopy and image analysis software to quantitatively analyze and compare R. palustris biofilm formation over time in these two metabolic modes. We describe quantifiable differences in structure between the biofilms formed by the bacterium grown heterotrophically and those grown photoheterotrophically. We developed a computational model to explore ways in which biotic and abiotic parameters could drive the observed biofilm architectures, as well as a random-forest machine-learning algorithm based on structural differences that was able to identify growth conditions from the confocal imaging of the biofilms with 87% accuracy. Insight into the structure of phototrophic biofilms and conditions that influence biofilm formation is relevant for understanding the generation of biofilm structures with different properties, and for optimizing applications with phototrophic bacteria growing in the biofilm state.

  11. Evolution of low-light adapted peripheral light-harvesting complexes in strains of Rhodopseudomonas palustris.

    Science.gov (United States)

    Kotecha, Abhay; Georgiou, Theonie; Papiz, Miroslav Z

    2013-03-01

    Purple bacteria have peripheral light-harvesting (PLH) complexes adapted to high-light (LH2) and low-light (LH3, LH4) growth conditions. The latter two have only been fully characterised in Rhodopseudomonas acidophila 7050 and Rhodopseudomonas palustris CGA009, respectively. It is known that LH4 complexes are expressed under the control of two light sensing bacteriophytochromes (BphPs). Recent genomic sequencing of a number of Rps. palustris strains has provided extensive information on PLH genes. We show that both LH3 and LH4 complexes are present in Rps. palustris and have evolved in the same operon controlled by the two adjacent BphPs. Two rare marker genes indicate that a gene cluster CL2, containing LH2 genes and the BphP RpBphP4, was internally transferred within the genome to form a new operon CL1. In CL1, RpBphP4 underwent gene duplication to RpBphP2 and RpBphP3, which evolved to sense light intensity rather than spectral red/far-red intensity ratio. We show that a second LH2 complex was acquired in CL1 belonging to a different PLH clade and these two PLH complexes co-evolved together into LH3 or LH4 complexes. The near-infrared spectra provide additional support for our conclusions on the evolution of PLH complexes based on genomic data.

  12. Biohydrogen production from CO-rich syngas via a locally isolated Rhodopseudomonas palustris PT.

    Science.gov (United States)

    Pakpour, Fatemeh; Najafpour, Ghasem; Tabatabaei, Meisam; Tohidfar, Masoud; Younesi, Habiboallah

    2014-05-01

    Biohydrogen production through water–gas shift (WGS) reaction by a biocatalyst was conducted in batch fermentation. The isolated photosynthetic bacterium Rhodopseudomonas palustris PT was able to utilize carbon monoxide and simultaneously produce hydrogen. Light exposure was provided as an indispensable requirement for the first stage of bacterial growth, but throughout the hydrogen production stage, the energy requirement was met through the WGS reaction. At ambient pressure and temperature, the effect of various sodium acetate concentrations in presence of CO-rich syngas on cell growth, carbon monoxide consumption, and biohydrogen production was also investigated. Maximal efficiency of hydrogen production in response to carbon monoxide consumption was recorded at 86 % and the highest concentration of hydrogen at 33.5 mmol/l was achieved with sodium acetate concentration of 1.5 g/l. The obtained results proved that the local isolate; R. palustris PT, was able to utilize CO-rich syngas and generate biohydrogen via WGS reaction.

  13. Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris.

    Science.gov (United States)

    Austin, Samantha; Kontur, Wayne S; Ulbrich, Arne; Oshlag, J Zachary; Zhang, Weiping; Higbee, Alan; Zhang, Yaoping; Coon, Joshua J; Hodge, David B; Donohue, Timothy J; Noguera, Daniel R

    2015-07-21

    Lignocellulosic biomass hydrolysates hold great potential as a feedstock for microbial biofuel production, due to their high concentration of fermentable sugars. Present at lower concentrations are a suite of aromatic compounds that can inhibit fermentation by biofuel-producing microbes. We have developed a microbial-mediated strategy for removing these aromatic compounds, using the purple nonsulfur bacterium Rhodopseudomonas palustris. When grown photoheterotrophically in an anaerobic environment, R. palustris removes most of the aromatics from ammonia fiber expansion (AFEX) treated corn stover hydrolysate (ACSH), while leaving the sugars mostly intact. We show that R. palustris can metabolize a host of aromatic substrates in ACSH that have either been previously described as unable to support growth, such as methoxylated aromatics, and those that have not yet been tested, such as aromatic amides. Removing the aromatics from ACSH with R. palustris, allowed growth of a second microbe that could not grow in the untreated ACSH. By using defined mutants, we show that most of these aromatic compounds are metabolized by the benzoyl-CoA pathway. We also show that loss of enzymes in the benzoyl-CoA pathway prevents total degradation of the aromatics in the hydrolysate, and instead allows for biological transformation of this suite of aromatics into selected aromatic compounds potentially recoverable as an additional bioproduct.

  14. Insights into arsenic multi-operons expression and resistance mechanisms in Rhodopseudomonas palustris CGA009

    Science.gov (United States)

    Zhao, Chungui; Zhang, Yi; Chan, Zhuhua; Chen, Shicheng; Yang, Suping

    2015-01-01

    Arsenic (As) is widespread in the environment and causes numerous health problems. Rhodopseudomonas palustris has been regarded as a good model organism for studying arsenic detoxification since it was first demonstrated to methylate environmental arsenic by conversion to soluble or gaseous methylated species. However, the detailed arsenic resistance mechanisms remain unknown though there are at least three arsenic-resistance operons (ars1, ars2, and ars3) in R. palustris. In this study, we investigated how arsenic multi-operons contributed to arsenic detoxification in R. palustris. The expression of ars2 or ars3 operons increased with increasing environmental arsenite (As(III)) concentrations (up to 1.0 mM) while transcript of ars1 operon was not detected in the middle log-phase (55 h). ars2 operon was actively expressed even at the low concentration of As(III) (0.01 μM), whereas the ars3 operon was expressed at 1.0 μM of As(III), indicating that there was a differential regulation mechanism for the three arsenic operons. Furthermore, ars2 and ars3 operons were maximally transcribed in the early log-phase where ars2 operon was 5.4-fold higher than that of ars3 operon. A low level of ars1 transcript was only detected at 43 h (early log-phase). Arsenic speciation analysis demonstrated that R. palustris could reduce As(V) to As(III). Collectively, strain CGA009 detoxified arsenic by using arsenic reduction and methylating arsenic mechanism, while the latter might occur with the presence of higher concentrations of arsenic. PMID:26441915

  15. Crystal structure of the RC-LH1 core complex from Rhodopseudomonas palustris.

    Science.gov (United States)

    Roszak, Aleksander W; Howard, Tina D; Southall, June; Gardiner, Alastair T; Law, Christopher J; Isaacs, Neil W; Cogdell, Richard J

    2003-12-12

    The crystal structure at 4.8 angstrom resolution of the reaction center-light harvesting 1 (RC-LH1) core complex from Rhodopseudomonas palustris shows the reaction center surrounded by an oval LH1 complex that consists of 15 pairs of transmembrane helical alpha- and beta-apoproteins and their coordinated bacteriochlorophylls. Complete closure of the RC by the LH1 is prevented by a single transmembrane helix, out of register with the array of inner LH1 alpha-apoproteins. This break, located next to the binding site in the reaction center for the secondary electron acceptor ubiquinone (UQB), may provide a portal through which UQB can transfer electrons to cytochrome b/c1.

  16. Uranium interaction with two multi-resistant environmental bacteria: Cupriavidus metallidurans CH34 and Rhodopseudomonas palustris.

    Directory of Open Access Journals (Sweden)

    Isabelle Llorens

    Full Text Available Depending on speciation, U environmental contamination may be spread through the environment or inversely restrained to a limited area. Induction of U precipitation via biogenic or non-biogenic processes would reduce the dissemination of U contamination. To this aim U oxidation/reduction processes triggered by bacteria are presently intensively studied. Using X-ray absorption analysis, we describe in the present article the ability of Cupriavidus metallidurans CH34 and Rhodopseudomonas palustris, highly resistant to a variety of metals and metalloids or to organic pollutants, to withstand high concentrations of U and to immobilize it either through biosorption or through reduction to non-uraninite U(IV-phosphate or U(IV-carboxylate compounds. These bacterial strains are thus good candidates for U bioremediation strategies, particularly in the context of multi-pollutant or mixed-waste contaminations.

  17. Hydrogen production under salt stress conditions by a freshwater Rhodopseudomonas palustris strain.

    Science.gov (United States)

    Adessi, Alessandra; Concato, Margherita; Sanchini, Andrea; Rossi, Federico; De Philippis, Roberto

    2016-03-01

    Hydrogen represents a possible alternative energy carrier to face the growing request for energy and the shortage of fossil fuels. Photofermentation for the production of H2 constitutes a promising way for integrating the production of energy with waste treatments. Many wastes are characterized by high salinity, and polluted seawater can as well be considered as a substrate. Moreover, the application of seawater for bacterial culturing is considered cost-effective. The aims of this study were to assess the capability of the metabolically versatile freshwater Rhodopseudomonas palustris 42OL of producing hydrogen on salt-containing substrates and to investigate its salt stress response strategy, never described before. R. palustris 42OL was able to produce hydrogen in media containing up to 3 % added salt concentration and to grow in media containing up to 4.5 % salinity without the addition of exogenous osmoprotectants. While the hydrogen production performances in absence of sea salts were higher than in their presence, there was no significant difference in performances between 1 and 2 % of added sea salts. Nitrogenase expression levels indicated that the enzyme was not directly inhibited during salt stress, but a regulation of its expression may have occurred in response to salt concentration increase. During cell growth and hydrogen production in the presence of salts, trehalose was accumulated as a compatible solute; it protected the enzymatic functionality against salt stress, thus allowing hydrogen production. The possibility of producing hydrogen on salt-containing substrates widens the range of wastes that can be efficiently used in production processes.

  18. How posttranslational modification of nitrogenase is circumvented in Rhodopseudomonas palustris strains that produce hydrogen gas constitutively.

    Science.gov (United States)

    Heiniger, Erin K; Oda, Yasuhiro; Samanta, Sudip K; Harwood, Caroline S

    2012-02-01

    Nitrogenase catalyzes the conversion of dinitrogen gas (N(2)) and protons to ammonia and hydrogen gas (H(2)). This is a catalytically difficult reaction that requires large amounts of ATP and reducing power. Thus, nitrogenase is not normally expressed or active in bacteria grown with a readily utilized nitrogen source like ammonium. nifA* mutants of the purple nonsulfur phototrophic bacterium Rhodopseudomonas palustris have been described that express nitrogenase genes constitutively and produce H(2) when grown with ammonium as a nitrogen source. This raised the regulatory paradox of why these mutants are apparently resistant to a known posttranslational modification system that should switch off the activity of nitrogenase. Microarray, mutation analysis, and gene expression studies showed that posttranslational regulation of nitrogenase activity in R. palustris depends on two proteins: DraT2, an ADP-ribosyltransferase, and GlnK2, an NtrC-regulated P(II) protein. GlnK2 was not well expressed in ammonium-grown NifA* cells and thus not available to activate the DraT2 nitrogenase modification enzyme. In addition, the NifA* strain had elevated nitrogenase activity due to overexpression of the nif genes, and this increased amount of expression overwhelmed a basal level of activity of DraT2 in ammonium-grown cells. Thus, insufficient levels of both GlnK2 and DraT2 allow H(2) production by an nifA* mutant grown with ammonium. Inactivation of the nitrogenase posttranslational modification system by mutation of draT2 resulted in increased H(2) production by ammonium-grown NifA* cells.

  19. In vitro assessment of gastrointestinal viability of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaeroides

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The objectives of this study were to assess the potential of two photosynthetic bacteria (PSB), Rhodopseudomonas palustris HZ0301 and Rhodobacter sphaeroides HZ0302, as probiotics in aquaculture. The viability of HZ0301 and HZ0302 in simulated gastric transit conditions (pH 2.0, pH 3.0 and pH 4.0 gastric juices) and in simulated small intestinal transit conditions (pH 8.0, with or without 0.3% bile salts) was tested. The effects of HZ0301 and HZ0302 on the viability and permeability of intestinal epithelial cell in primary culture of tilapias, Oreochromis nilotica, were also detected. All the treatments were determined with three replicates. The simulated gastric transit tolerance of HZ0301 and HZ0302 strains was pH-dependent and correspondingly showed lower viability at pH 2.0 after 180 min compared with pH 3.0 and pH 4.0. Both HZ0301 and HZ0302 were tolerant to simulated small intestine transit with or without bile salts in our research. Moreover, there was no significant difference (P>0.05) among three treatments including the control and the groups treated with HZ0301 or HZ0302 both in intestinal epithelial cell viability and membrane permeability, showing no cell damage. In summary, this study demonstrated that HZ0301 and HZ0302 had high capacity of upper gastrointestinal transit tolerance and were relatively safe for intestinal epithelial cells of tilapias.

  20. Hydrogen Photoproduction by Rhodopseudomonas palustris 42OL Cultured at High Irradiance under a Semicontinuous Regime

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    Pietro Carlozzi

    2012-01-01

    Full Text Available The main goal of this study was to increase the hydrogen production rate improving the culture technique and the photobioreactor performances. Experiments were carried out at a constant culture temperature of 30°C and at an average irradiance of 480 W m−2 using a cylindrical photobioreactor (4.0 cm, internal diameter. The culture technique, namely, the semicontinuous regime for growing Rhodopseudomonas palustris 42OL made it possible to achieve a very high daily hydrogen production rate of 594 ± 61 mL (H2 L−1 d−1. This value, never reported for this strain, corresponds to about 25 mL (H2 L−1 h−1, and it was obtained when the hydraulic retention time (HRT was of 225 hours. Under the same growth conditions, a very high biomass production rate (496 ± 45 mg (dw L−1 d−1 was also achieved. Higher or lower HRTs caused a reduction in both the hydrogen and the biomass production rates. The malic-acid removal efficiency (MAre was always higher than 90%. The maximal hydrogen yield was 3.03 mol H2 mol MA−1 at the HRT of 360 hours. The highest total energy conversion efficiency was achieved at the HRT of 225 hours.

  1. Physiology and Mechanism of Phototrophic Fe(II) Oxidation by Rhodopseudomonas palustris TIE-1

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    Jiao, Y.; Newman, D.

    2007-12-01

    Phototrophic Fe(II)-oxidizing bacteria use electrons from ferrous iron [Fe(II)] and energy from light to drive reductive CO2 fixation. This metabolism is thought to be ancient in origin, and plays an important role in environmental iron cycling. It has been implicated in the deposition of Banded Iron Formations, a class of ancient sedimentary iron deposits. Consistent with this hypothesis, we discovered that hydrogen gas, a thermodynamically favorable electron donor to Fe(II), in an Archean atmosphere would not have inhibited phototrophic Fe(II) oxidation. To understand this physiology and the connection to BIF formation at the molecular level, the mechanisms of phototrophic Fe(II) oxidation were examined in a model organism Rhodopseudomonas palustris TIE-1. Increased expression of a putative decaheme c-type cytochrome, encoded by pioA, was observed when cells were grown under Fe(II)-oxidizing conditions. Two genes located immediately downstream of pioA in the same operon, pioB and pioC, encode a putative outer membrane beta-barrel protein and a putative high potential iron-sulfur protein, respectively. Deletion studies demonstrated that all three genes are involved in phototrophic Fe(II) oxidation. This study provides our first insight into the molecular mechanisms of this metabolism, which will be further characterized by in vitro biochemical studies.

  2. Statistical considerations on the formation of circular photosynthetic light-harvesting complexes from Rhodopseudomonas palustris.

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    Taniguchi, Masahiko; Henry, Sarah; Cogdell, Richard J; Lindsey, Jonathan S

    2014-07-01

    Depending on growth conditions, some species of purple photosynthetic bacteria contain peripheral light-harvesting (LH2) complexes that are heterogeneous owing to the presence of different protomers (containing different αβ-apoproteins). Recent spectroscopic studies of Rhodopseudomonas palustris grown under low-light conditions suggest the presence of a C 3-symmetric LH2 nonamer comprised of two distinct protomers. The software program Cyclaplex, which enables generation and data-mining of virtual libraries of molecular rings formed upon combinatorial reactions, has been used to delineate the possible number and type of distinct nonamers as a function of numbers of distinct protomers. The yield of the C 3-symmetric nonamer from two protomers (A and B in varying ratios) has been studied under the following conditions: (1) statistical, (2) enriched (preclusion of the B-B sequence), and (3) seeded (pre-formation of an A-B-A block). The yield of C 3-symmetric nonamer is at most 0.98 % under statistical conditions versus 5.6 % under enriched conditions, and can be dominant under conditions of pre-seeding with an A-B-A block. In summary, the formation of any one specific nonamer even from only two protomers is unlikely on statistical grounds but must stem from enhanced free energy of formation or a directed assembly process by as-yet unknown factors.

  3. Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol.

    Science.gov (United States)

    Doud, Devin F R; Holmes, Eric C; Richter, Hanno; Molitor, Bastian; Jander, Georg; Angenent, Largus T

    2017-01-01

    Rhodopseudomonas palustris is a versatile microbe that encounters an innate redox imbalance while growing photoheterotrophically with reduced substrates. The resulting excess in reducing equivalents, together with ATP from photosynthesis, could be utilized to drive a wide range of bioconversions. The objective of this study was to genetically modify R. palustris to provide a pathway to reduce n-butyrate into n-butanol for maintaining redox balance. Here, we constructed and expressed a plasmid-based pathway for n-butanol production from Clostridium acetobutylicum ATCC 824 in R. palustris. We maintained the environmental conditions in such a way that this pathway functioned as the obligate route to re-oxidize excess reducing equivalents, resulting in an innate selection pressure. The engineered strain of R. palustris grew under otherwise restrictive redox conditions and achieved concentrations of 1.5 mM n-butanol at a production rate of 0.03 g L(-1) day(-1) and a selectivity (i.e., products compared to the consumed substrate) of close to 40%. Since the theoretical maximum selectivity is 45%, the engineered strain converted close to its maximum selectivity. The innate redox imbalance of R. palustris can be used to drive the reduction of n-butyrate into n-butanol after expression of a plasmid-based enzyme from a butanol-producing Clostridium strain.

  4. Continuous photo-hydrogen production from acetate using Rhodopseudomonas palustris WP 3-5

    Energy Technology Data Exchange (ETDEWEB)

    Yeh, Kuei-Ling; Lo, Yung-Chung [National Cheng Kung Univ., Tainan, Taiwan (China). Dept. of Chemical Engineering; Chen, Chun-Yen [National Cheng Kung Univ., Tainan, Taiwan (China). Dept. of Chemical Engineering; National Cheng Kung Univ., Tainan, Taiwan (China). Sustainable Environment Research Centre; Chang, Jo-Shu [National Cheng Kung Univ., Tainan, Taiwan (China). Dept. of Chemical Engineering; National Cheng Kung Univ., Tainan, Taiwan (China). Sustainable Environment Research Centre; National Cheng Kung Univ., Tainan, Taiwan (China). Microalgae Biotechnology and Bioengineering Lab.

    2010-07-01

    Phototropic hydrogen production using components of dark fermentation metabolites (i.e. acetate) is an ideal biohydrogen production route since it enables the highest yield possible (i.e., 12 mol H{sub 2}/mol hexose) and could also reduce COD content in the dark fermentation effluent to mitigate the environmental burden. In this work, a purple nonsulfur photosynthetic bacterial isolate Rhodopseudomonas palustris WP3-5 was utilized to produce H{sub 2}. To assess the performance of the photo-H{sub 2} production system, the continuous photobioreactor was operated at a hydraulic retention time (HRT) of 48 h under different acetate concentrations. The optimal H{sub 2} production efficiency occurred when the acetate concentration was 3000 mg COD/l, leading to an overall H{sub 2} production rate and a H{sub 2} yield of 20.4 ml/h/l and 0.98 mol H{sub 2}/mol acetate, respectively. In all tests, the acetate conversion was nearly constant at 87.0{+-}3.1%. Next, the preferable HRT leading to the best H{sub 2} production performance was identified. The results show that at an acetate concentration of 3000 mg COD/L, H{sub 2} production rate increased with a decrease in HRT, giving the best performance at HRT=12 h with an overall H{sub 2} production rate of 42.05 ml/h/l and a H{sub 2} yield of 1.39 mol H{sub 2}/mol acetate. Operation under the aforementioned optimal substrate concentration and HRT, the overall H{sub 2} production rate could be enhanced two-fold higher when compared with that obtained from preliminary tests. In addition, the HRT setting was found to play a key role in influencing the performance of continuous H{sub 2} production by R. palustris WP3-5. The proposed continuous cultures seem to be a favourable choice of bioreactor strategy, possessing the potential to achieve higher H{sub 2} production efficiency with an excellent stability. (orig.)

  5. A novel electrophototrophic bacterium Rhodopseudomonas palustris strain RP2, exhibits hydrocarbonoclastic potential in anaerobic environments

    Directory of Open Access Journals (Sweden)

    Krishnaveni Venkidusamy

    2016-07-01

    Full Text Available An electrophototrophic, hydrocarbonoclastic bacterium Rhodopseudomonas palustris stain RP2 was isolated from the anodic biofilms of hydrocarbon fed microbial electrochemical remediation systems (MERS. Salient properties of the strain RP2 were direct electrode respiration, dissimilatory metal oxide reduction, spore formation, anaerobic nitrate reduction, free living diazotrophy and the ability to degrade n-alkane components of petroleum hydrocarbons in anoxic, photic environments. In acetate fed microbial electrochemical cells, a maximum current density of 305±10 mA/m2 (1000Ω was generated (power density 131.65±10 mW/m2 by strain RP2 with a coulombic efficiency of 46.7 ± 1.3%. Cyclic voltammetry studies showed that anaerobically grown cells of strain RP2 is electrochemically active and likely to transfer electrons extracellularly to solid electron acceptors through membrane bound compounds, however, aerobically grown cells lacked the electrochemical activity. The ability of strain RP2 to produce current (maximum current density 21±3 mA/m2; power density 720±7 µW/m2, 1000Ω using petroleum hydrocarbon (PH as a sole energy source was also examined using an initial concentration of 800 mg l-1 of diesel range hydrocarbons (C9- C36 with a concomitant removal of 47.4 ± 2.7% hydrocarbons in MERS. Here, we also report the first study that shows an initial evidence for the existence of a hydrocarbonoclastic behavior in the strain RP2 when grown in different electron accepting and illuminated conditions (anaerobic and MERS degradation. Such observations reveal the importance of photoorganotrophic growth in the utilization of hydrocarbons from contaminated environments. Identification of such novel petrochemical hydrocarbon degrading electricigens, not only expands the knowledge on the range of bacteria known for the hydrocarbon bioremediation but also shows a biotechnological potential that goes well beyond its applications to MERS.

  6. Effects of pH on the peripheral light-harvesting antenna complex for Rhodopseudomonas palustris

    Institute of Scientific and Technical Information of China (English)

    FENG Juan; LI XueFeng; LIU Yan

    2008-01-01

    In this work steady-state absorption spectroscopy, circular dichroism spectroscopy and sub-micro-second time-resolved absorption spectroscopy were used to investigate the effect of pH on the struc-tures and functions of LH2 complex for Rhodopseudomonas palustris. The results revealed that: (1) B800 Bchla was gradually transformed to free pigments absorbing around 760 nm on the minutes timescale upon the induction of strong acidic pH, and subsequently there disappeared the CD signal for Qy band of B800 in the absence of B800. In addition, Carotenoids changed with the similar tendency to B850 BChl. (2) The introduction of strong basic pH gave rise to no significant changes for B800 Bchla, while B850 BChla experienced remarkable spectral blue-shift from 852 to 837 nm. Similar phe-nomenon was seen for the CD signal for Qy band of B850. Carotenoids displayed strong and pH-independent CD signals in the visible range. (3) In the case of both physiological and basic pH, broad and asymmetrical positive Tn←T1 transient absorption appeared following the pulsed photo-excitation of Car at 532 nm. By contrast, the featureless and weak positive signal was observed on the sub-microsecond timescale in the acidic pH environment. The aforementioned experimental results indicated that acidic pH-induced removal of B800 Bchla prevented the generation of the caro-tenoid triplet state (3Car*), which is known to be essential for the photo-protection function. Neverthe-less, carotenoids can still perform this important physiological role under the basic pH condition, where the spectral blue shift of B850 exerts little effect on the overall structure of the cyclic aggregate, therefore favoring the formation of carotenoid triplet state.

  7. Low light adaptation: energy transfer processes in different types of light harvesting complexes from Rhodopseudomonas palustris.

    Science.gov (United States)

    Moulisová, Vladimíra; Luer, Larry; Hoseinkhani, Sajjad; Brotosudarmo, Tatas H P; Collins, Aaron M; Lanzani, Guglielmo; Blankenship, Robert E; Cogdell, Richard J

    2009-12-01

    Energy transfer processes in photosynthetic light harvesting 2 (LH2) complexes isolated from purple bacterium Rhodopseudomonas palustris grown at different light intensities were studied by ground state and transient absorption spectroscopy. The decomposition of ground state absorption spectra shows contributions from B800 and B850 bacteriochlorophyll (BChl) a rings, the latter component splitting into a low energy and a high energy band in samples grown under low light (LL) conditions. A spectral analysis reveals strong inhomogeneity of the B850 excitons in the LL samples that is well reproduced by an exponential-type distribution. Transient spectra show a bleach of both the low energy and high energy bands, together with the respective blue-shifted exciton-to-biexciton transitions. The different spectral evolutions were analyzed by a global fitting procedure. Energy transfer from B800 to B850 occurs in a mono-exponential process and the rate of this process is only slightly reduced in LL compared to high light samples. In LL samples, spectral relaxation of the B850 exciton follows strongly nonexponential kinetics that can be described by a reduction of the bleach of the high energy excitonic component and a red-shift of the low energetic one. We explain these spectral changes by picosecond exciton relaxation caused by a small coupling parameter of the excitonic splitting of the BChl a molecules to the surrounding bath. The splitting of exciton energy into two excitonic bands in LL complex is most probably caused by heterogenous composition of LH2 apoproteins that gives some of the BChls in the B850 ring B820-like site energies, and causes a disorder in LH2 structure.

  8. Low light adaptation: Energy transfer processes in different types of light harvesting complexes from Rhodopseudomonas palustris

    Energy Technology Data Exchange (ETDEWEB)

    Moulisova, Vladimira; Luer, Larry; Hoseinkhani, Sajjad; Brotosudarmo, Tatas H.P.; Collins, Aaron M.; Lanzani, Guglielmo; Blankenship, R. E.; Cogdell, Richard J

    Energy transfer processes in photosynthetic light harvesting 2 (LH2) complexes isolated from purple bacterium Rhodopseudomonas palustris grown at different light intensities were studied by ground state and transient absorption spectroscopy. The decomposition of ground state absorption spectra shows contributions from B800 and B850 bacteriochlorophyll (BChl) a rings, the latter component splitting into a low energy and a high energy band in samples grown under low light (LL) conditions. A spectral analysis reveals strong inhomogeneity of the B850 excitons in the LL samples that is well reproduced by an exponential-type distribution. Transient spectra show a bleach of both the low energy and high energy bands, together with the respective blue-shifted exciton-to-biexciton transitions. The different spectral evolutions were analyzed by a global fitting procedure. Energy transfer from B800 to B850 occurs in a mono-exponential process and the rate of this process is only slightly reduced in LL compared to high light samples. In LL samples, spectral relaxation of the B850 exciton follows strongly nonexponential kinetics that can be described by a reduction of the bleach of the high energy excitonic component and a red-shift of the low energetic one. We explain these spectral changes by picosecond exciton relaxation caused by a small coupling parameter of the excitonic splitting of the BChl a molecules to the surrounding bath. The splitting of exciton energy into two excitonic bands in LL complex is most probably caused by heterogenous composition of LH2 apoproteins that gives some of the BChls in the B850 ring B820-like site energies, and causes a disorder in LH2 structure.

  9. Phenotype fingerprinting suggests the involvement of single-genotype consortia in degradation of aromatic compounds by Rhodopseudomonas palustris.

    Directory of Open Access Journals (Sweden)

    Tatiana V Karpinets

    Full Text Available Anaerobic degradation of complex organic compounds by microorganisms is crucial for development of innovative biotechnologies for bioethanol production and for efficient degradation of environmental pollutants. In natural environments, the degradation is usually accomplished by syntrophic consortia comprised of different bacterial species. This strategy allows consortium organisms to reduce efforts required for maintenance of the redox homeostasis at each syntrophic level. Cellular mechanisms that maintain the redox homeostasis during the degradation of aromatic compounds by one organism are not fully understood. Here we present a hypothesis that the metabolically versatile phototrophic bacterium Rhodopseudomonas palustris forms its own syntrophic consortia, when it grows anaerobically on p-coumarate or benzoate as a sole carbon source. We have revealed the consortia from large-scale measurements of mRNA and protein expressions under p-coumarate, benzoate and succinate degrading conditions using a novel computational approach referred as phenotype fingerprinting. In this approach, marker genes for known R. palustris phenotypes are employed to determine the relative expression levels of genes and proteins in aromatics versus non-aromatics degrading condition. Subpopulations of the consortia are inferred from the expression of phenotypes and known metabolic modes of the R. palustris growth. We find that p-coumarate degrading conditions may lead to at least three R. palustris subpopulations utilizing p-coumarate, benzoate, and CO2 and H2. Benzoate degrading conditions may also produce at least three subpopulations utilizing benzoate, CO2 and H2, and N2 and formate. Communication among syntrophs and inter-syntrophic dynamics in each consortium are indicated by up-regulation of transporters and genes involved in the curli formation and chemotaxis. The N2-fixing subpopulation in the benzoate degrading consortium has preferential activation of the

  10. Promoting effects of a single Rhodopseudomonas palustris inoculant on plant growth by Brassica rapa chinensis under low fertilizer input.

    Science.gov (United States)

    Wong, Wai-Tak; Tseng, Ching-Han; Hsu, Shu-Hua; Lur, Huu-Sheng; Mo, Chia-Wei; Huang, Chu-Ning; Hsu, Shu-Chiung; Lee, Kung-Ta; Liu, Chi-Te

    2014-09-17

    Several Rhodopseudomonas palustris strains have been isolated from rice paddy fields in Taiwan by combining the Winogradsky column method and molecular marker detection. These isolates were initially screened by employing seed germination and seedling vigor assays to evaluate their potential as inoculants. To fulfill the demand in the present farming system for reducing the application of chemical fertilizers, we assessed the plant growth-promoting effects of the R. palustris YSC3, YSC4, and PS3 inoculants on Brassica rapa chinensis (Chinese cabbage) cultivated under a half quantity of fertilizer. The results obtained showed that supplementation with approximately 4.0×10(6) CFU g(-1) soil of the PS3 inoculant at half the amount of fertilizer consistently produced the same plant growth potential as 100% fertility, and also increased the nitrogen use efficiency of the applied fertilizer nutrients. Furthermore, we noted that the plant growth-promotion rate elicited by PS3 was markedly higher with old seeds than with new seeds, suggesting it has the potential to boost the development of seedlings that were germinated from carry-over seeds of poor quality. These beneficial traits suggest that the PS3 isolate may serve as a potential PGPR inoculant for integrated nutrient management in agriculture.

  11. Promoting Effects of a Single Rhodopseudomonas palustris Inoculant on Plant Growth by Brassica rapa chinensis under Low Fertilizer Input

    Science.gov (United States)

    Wong, Wai-Tak; Tseng, Ching-Han; Hsu, Shu-Hua; Lur, Huu-Sheng; Mo, Chia-Wei; Huang, Chu-Ning; Hsu, Shu-Chiung; Lee, Kung-Ta; Liu, Chi-Te

    2014-01-01

    Several Rhodopseudomonas palustris strains have been isolated from rice paddy fields in Taiwan by combining the Winogradsky column method and molecular marker detection. These isolates were initially screened by employing seed germination and seedling vigor assays to evaluate their potential as inoculants. To fulfill the demand in the present farming system for reducing the application of chemical fertilizers, we assessed the plant growth-promoting effects of the R. palustris YSC3, YSC4, and PS3 inoculants on Brassica rapa chinensis (Chinese cabbage) cultivated under a half quantity of fertilizer. The results obtained showed that supplementation with approximately 4.0×106 CFU g−1 soil of the PS3 inoculant at half the amount of fertilizer consistently produced the same plant growth potential as 100% fertility, and also increased the nitrogen use efficiency of the applied fertilizer nutrients. Furthermore, we noted that the plant growth-promotion rate elicited by PS3 was markedly higher with old seeds than with new seeds, suggesting it has the potential to boost the development of seedlings that were germinated from carry-over seeds of poor quality. These beneficial traits suggest that the PS3 isolate may serve as a potential PGPR inoculant for integrated nutrient management in agriculture. PMID:25130882

  12. Single-genotype syntrophy by Rhodopseudomonas palustris is not a strategy to aid redox balance during anaerobic degradation of lignin monomers

    Directory of Open Access Journals (Sweden)

    Devin F. R. Doud

    2016-07-01

    Full Text Available Rhodopseudomonas palustris has emerged as a model microbe for the anaerobic metabolism of p-coumarate, which is an aromatic compound and a primary component of lignin. However, under anaerobic conditions, R. palustris must actively eliminate excess reducing equivalents through a number of known strategies (e.g., CO2 fixation, H2 evolution to avoid lethal redox imbalance. Others had hypothesized that to ease the burden of this redox imbalance, a clonal population of R. palustris could functionally differentiate into a pseudo-consortium. Within this pseudo-consortium, one sub-population would perform the aromatic moiety degradation into acetate, while the other sub-population would oxidize acetate, resulting in a single-genotype syntrophy through acetate sharing. Here, the objective was to test this hypothesis by utilizing microbial electrochemistry as a research tool with the extracellular-electron-transferring bacterium Geobacter sulfurreducens as a reporter strain replacing the hypothesized acetate-oxidizing sub-population. We used a 2x4 experimental design with pure cultures of R. palustris in serum bottles and co-cultures of R. palustris and G. sulfurreducens in bioelectrochemical systems. This experimental design included growth medium with and without bicarbonate to induce non-lethal and lethal redox imbalance conditions, respectively, in R. palustris. Finally, the design also included a mutant strain (NifA* of R. palustris, which constitutively produces H2, to serve both as a positive control for metabolite secretion (H2 to G. sulfurreducens, and as a non-lethal redox control for without bicarbonate conditions. Our results demonstrate that acetate sharing between different sub-populations of R. palustris does not occur while degrading p-coumarate under either non-lethal or lethal redox imbalance conditions. This work highlights the strength of microbial electrochemistry as a tool for studying microbial syntrophy.

  13. Phototrophic Growth and Accumulation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate by Purple Nonsulfur Bacterium Rhodopseudomonas palustris SP5212

    Directory of Open Access Journals (Sweden)

    M. Mukhopadhyay

    2013-01-01

    Full Text Available The ability of the phototrophic bacterium Rhodopseudomonas palustris SP5212 to produce polyhydroxyalkanoates (PHAs, poly(3-hydroxybutyrate-co-3-hydroxyvalerate [P(3HB-co-3HV] in particular was, assessed in acetate medium supplemented with hydroxybutyrate and valerate as cosubstrates. The isolate accumulated the polymer accounting for some 49.06% and 30% of cell dry weight when grown in hydroxybutyrate and valerate, respectively. PHA accumulation as well as 3HV monomer incorporation (30 mol% was maximum at 0.1% hydroxybutyrate, while valerate at 0.1% and 0.3% was suitable for total polymer accumulation and 3HV monomer incorporation, respectively. Cosupplementation of hydroxybutyrate and valerate in the ratio of 3 : 1 led to the accumulation of PHA accounting for 54% of cell dry weight, which contained more than 50 mol% of 3HV monomer. Moreover, the biphasic cultivation conditions with hydroxybutyrate as cosubstrate have improved the quality as well as quantity of the accumulated copolymer significantly.

  14. Antisense RNA that Affects Rhodopseudomonas palustris Quorum-Sensing Signal Receptor Expression

    Science.gov (United States)

    2012-01-01

    terminal region of the Vibrio fischeri LuxR pro- tein contains an inducer-independent lux gene activating domain. Proc Natl Acad Sci USA 88:11115...synthesis requires exogenous p-coumarate (1). The R. palustris signal synthesis gene , rpaI, codes for a member of the large LuxI family of AHL synthases, and...the adjacent signal receptor gene , rpaR, codes for a member of the LuxR family of transcription factors. Although rpaI and rpaR are not cotran

  15. Evolution of KaiC-Dependent Timekeepers: A Proto-circadian Timing Mechanism Confers Adaptive Fitness in the Purple Bacterium Rhodopseudomonas palustris.

    Directory of Open Access Journals (Sweden)

    Peijun Ma

    2016-03-01

    Full Text Available Circadian (daily rhythms are a fundamental and ubiquitous property of eukaryotic organisms. However, cyanobacteria are the only prokaryotic group for which bona fide circadian properties have been persuasively documented, even though homologs of the cyanobacterial kaiABC central clock genes are distributed widely among Eubacteria and Archaea. We report the purple non-sulfur bacterium Rhodopseudomonas palustris (that harbors homologs of kaiB and kaiC only poorly sustains rhythmicity in constant conditions-a defining characteristic of circadian rhythms. Moreover, the biochemical characteristics of the Rhodopseudomonas homolog of the KaiC protein in vivo and in vitro are different from those of cyanobacterial KaiC. Nevertheless, R. palustris cells exhibit adaptive kaiC-dependent growth enhancement in 24-h cyclic environments, but not under non-natural constant conditions. Therefore, our data indicate that Rhodopseudomonas does not have a classical circadian rhythm, but a novel timekeeping mechanism that does not sustain itself in constant conditions. These results question the adaptive value of self-sustained oscillatory capability for daily timekeepers and establish new criteria for circadian-like systems that are based on adaptive properties (i.e., fitness enhancement in rhythmic environments, rather than upon observations of persisting rhythms in constant conditions. We propose that the Rhodopseudomonas system is a "proto" circadian timekeeper, as in an ancestral system that is based on KaiC and KaiB proteins and includes some, but not necessarily all, of the canonical properties of circadian clocks. These data indicate reasonable intermediate steps by which bona fide circadian systems evolved in simple organisms.

  16. High-throughput transcriptome sequencing analysis provides preliminary insights into the biotransformation mechanism of Rhodopseudomonas palustris treated with alpha-rhamnetin-3-rhamnoside.

    Science.gov (United States)

    Bi, Lei; Guan, Chun-jie; Yang, Guan-e; Yang, Fei; Yan, Hong-yu; Li, Qing-shan

    2016-04-01

    The purple photosynthetic bacterium Rhodopseudomonas palustris has been widely applied to enhance the therapeutic effects of traditional Chinese medicine using novel biotransformation technology. However, comprehensive studies of the R. palustris biotransformation mechanism are rare. Therefore, investigation of the expression patterns of genes involved in metabolic pathways that are active during the biotransformation process is essential to elucidate this complicated mechanism. To promote further study of the biotransformation of R. palustris, we assembled all R. palustris transcripts using Trinity software and performed differential expression analysis of the resulting unigenes. A total of 9725, 7341 and 10,963 unigenes were obtained by assembling the alpha-rhamnetin-3-rhamnoside-treated R. palustris (RPB) reads, control R. palustris (RPS) reads and combined RPB&RPS reads, respectively. A total of 9971 unigenes assembled from the RPB&RPS reads were mapped to the nr, nt, Swiss-Prot, Gene Ontology (GO), Clusters of Orthologous Groups (COGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) (E-value <0.00001) databases using BLAST software. A total of 3360 unique differentially expressed genes (DEGs) in RPB versus RPS were identified, among which 922 unigenes were up-regulated and 2438 were down-regulated. The unigenes were mapped to the KEGG database, resulting in the identification of 7676 pathways among all annotated unigenes and 2586 pathways among the DEGs. Some sets of functional unigenes annotated to important metabolic pathways and environmental information processing were differentially expressed between the RPS and RPB samples, including those involved in energy metabolism (18.4% of total DEGs), carbohydrate metabolism (36.0% of total DEGs), ABC transport (6.0% of total DEGs), the two-component system (8.6% of total DEGs), cell motility (4.3% of total DEGs) and the cell cycle (1.5% of total DEGs). We also identified 19 transcripts annotated as hydrolytic

  17. The open, the closed, and the empty: time-resolved fluorescence spectroscopy and computational analysis of RC-LH1 complexes from Rhodopseudomonas palustris.

    Science.gov (United States)

    Beyer, Sebastian R; Müller, Lars; Southall, June; Cogdell, Richard J; Ullmann, G Matthias; Köhler, Jürgen

    2015-01-29

    We studied the time-resolved fluorescence of isolated RC-LH1 complexes from Rhodopseudomonas palustris as a function of the photon fluence and the repetition rate of the excitation laser. Both parameters were varied systematically over 3 orders of magnitude. On the basis of a microstate description we developed a quantitative model for RC-LH1 and obtained very good agreement between experiments and elaborate simulations based on a global master equation approach. The model allows us to predict the relative population of RC-LH1 complexes with the special pair in the neutral state or in the oxidized state P(+) and those complexes that lack a reaction center.

  18. Acetate-dependent photoheterotrophic growth and the differential requirement for the Calvin-Benson-Bassham reductive pentose phosphate cycle in Rhodobacter sphaeroides and Rhodopseudomonas palustris.

    Science.gov (United States)

    Laguna, Rick; Tabita, F Robert; Alber, Birgit E

    2011-02-01

    Rhodobacter sphaeroides ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO)-deletion strain 16 was capable of photoheterotrophic growth with acetate, while Rhodopseudomonas palustris RubisCO-deletion strain 2040 could not grow under these conditions. The reason for this difference lies in the fact that Rba. sphaeroides and Rps. palustris use different pathways for acetate assimilation, the ethylmalonyl-CoA pathway, and glyoxylate-bypass cycle, respectively. The ethylmalonyl-CoA pathway is distinct from the glyoxylate cycle as one molecule of CO(2) and one molecule of HCO(3) (-) per three molecules of acetyl-CoA are co-assimilated to form two malate molecules. The glyoxylate cycle directly converts two acetyl-CoA molecules to malate. Each pathway, therefore, also dictates at what point, CO(2) and reductant are consumed, thereby determining the requirement for the Calvin-Benson-Bassham reductive pentose phosphate cycle.

  19. The effect of dilution and L-malic acid addition on bio-hydrogen production with Rhodopseudomonas palustris from effluent of an acidogenic anaerobic reactor

    Energy Technology Data Exchange (ETDEWEB)

    Azbar, Nuri; Cetinkaya Dokgoz, F.Tuba [Ege University, Faculty of Engineering, Bioengineering Department, 35100 Izmir (Turkey)

    2010-05-15

    In this study, H{sub 2} was produced from cheese whey wastewater in a two-stage biological process: i) first stage; thermophilic dark fermentation ii) second stage; the photo fermentation using Rhodopseudomonas palustris strain DSM 127 (R. palustris). The effect of both dilution and addition of L-malic acid on the hydrogen production was investigated. Among the dilution rates used, 1/5 dilution ratio was found to produce the best hydrogen production (349 ml H{sub 2}/g COD{sub fed}). On the other hand, It was seen that the mixing the effluent with L-malic acid at increasing ratios had further positive effect and improved the hydrogen production significantly. It was concluded that dilution of the feeding helps to reduce the nitrogen content and the volatile fatty acid content that might be otherwise harmful to the photo-heterotrophic organisms. Overall hydrogen production yield (for dark + photo fermentation) was found to vary 2 and 10 mol H{sub 2}/mol lactose. Second conclusion is that cheese whey effluent should be mixed with a co-substrate containing L-malic acid such as apple juice processing effluents before fed into the photo fermentation reactor. (author)

  20. Preservation of H2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R. palustris latex coatings

    Energy Technology Data Exchange (ETDEWEB)

    Piskorska, M. [Univ. of South Carolina, Aiken, SC (United States); Soule, T. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Gosse, J. L. [North Carolina State Univ., Raleigh, NC (United States); Milliken, C. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Flickinger, M. C. [North Carolina State Univ., Raleigh, NC (United States); Smith, G. W. [Univ. of South Carolina, Aiken, SC (United States); Yeager, C. M. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2013-01-21

    To assess the applicability of latex cell coatings as an ‘off-the-shelf’ biocatalyst, the effect of osmoprotectants, temperature, humidity and O2 on preservation of H2 production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H2 production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H2 production activity, whereas considerable H2 production was still detected in sucrose- and trehalose-stabilized coatings. The relative humidity level at which the coatings were stored had a significant impact on the recovery and subsequent rates of H2 production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H2 (0–0.1% headspace accumulation), whereas those stored at < 5% humidity retained 27–53% of their H2 production activity after 8 weeks of storage. In conWhen stored in argon at < 5% humidity and room temperature, R. palustris coatings retained full H2 production activity for 3 months, implicating oxidative damage as a key factor limiting coating storage. Overall, the results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state.

  1. Characterization of pII Family (GlnK1, GlnK2, GlnB) Protein Uridylylation in Response to Nitrogen Availability for Rhodopseudomonas palustris

    Energy Technology Data Exchange (ETDEWEB)

    Connelly, Heather M [ORNL; Pelletier, Dale A [ORNL; Lu, Tse-Yuan [ORNL; Lankford, Patricia K [ORNL; Hettich, Robert {Bob} L [ORNL

    2006-01-01

    The GlnK and GlnB proteins are members of the pII signal transduction protein family, which is essential in nitrogen regulation due to this protein family's ability to sense internal cellular ammonium levels and control cellular response. The role of GlnK in nitrogen regulation has been studied in a variety of bacteria but previously has been uncharacterized in the purple nonsulfur anoxygenic phototropic bacterium Rhodopseudomonas palustris. R. palustris has tremendous metabolic versatility in its modes of energy generation and carbon metabolism, and it employs a sensitive nitrogen-ammonium regulation system that may vary from that of other commonly studied bacteria. In R. palustris, there are three annotated forms of pII proteins: GlnK1, GlnK2, and GlnB. Here we describe, for the first time, the characterization of GlnK1, GlnK2, and GlnB modifications as a response to nitrogen availability, thereby providing information about how this bacterium regulates the AmtB ammonium transporter and glutamine synthetase, which controls the rate of glutamate to glutamine conversion. Using a strategy of creating C-terminally tagged GlnK and GlnB proteins followed by tandem affinity purification in combination with top-down mass spectrometry, four isoforms of the GlnK2 and GlnB proteins and two isoforms of the GlnK1 protein were characterized at high resolution and mass accuracy. Wild-type or endogenous expression of all three proteins was also examined under normal ammonium conditions and ammonium starvation to ensure that the tagging and affinity purification methods employed did not alter the natural state of the proteins. All three proteins were found to undergo uridylylation under ammonium starvation conditions, presumably to regulate the AmtB ammonium transporter and glutamine synthetase. Under high-ammonium conditions, the GlnK1, GlnK2, and GlnB proteins are unmodified. This experimental protocol involving high-resolution mass spectrometry measurements of intact

  2. Single-Molecule Spectroscopy Reveals that Individual Low-Light LH2 Complexes from Rhodopseudomonas palustris 2.1.6. Have a Heterogeneous Polypeptide Composition

    Science.gov (United States)

    Brotosudarmo, Tatas H.P.; Kunz, Ralf; Böhm, Paul; Gardiner, Alastair T.; Moulisová, Vladimíra; Cogdell, Richard J.; Köhler, Jürgen

    2009-01-01

    Abstract Rhodopseudomonas palustris belongs to the group of purple bacteria that have the ability to produce LH2 complexes with unusual absorption spectra when they are grown at low-light intensity. This ability is often related to the presence of multiple genes encoding the antenna apoproteins. Here we report, for the first time to our knowledge, direct evidence that individual low-light LH2 complexes have a heterogeneous αβ-apoprotein composition that modulates the site energies of Bchl a molecules, producing absorption bands at 800, 820, and 850 nm. The arrangement of the Bchl a molecules in the “tightly coupled ring” can be modeled by nine αβ-Bchls dimers, such that the Bchls bound to six αβ-pairs have B820-like site energies and the remaining Bchl a molecules have B850-like site energies. Furthermore, the experimental data can only be satisfactorily modeled when these six αβ-pairs with B820 Bchl a molecules are distributed such that the symmetry of the assembly is reduced to C3. It is also clear from the measured single-molecule spectra that the energies of the electronically excited states in the mixed B820/850 ring are mainly influenced by diagonal disorder. PMID:19720038

  3. Evaluation of the effects of different liquid inoculant formulations on the survival and plant-growth-promoting efficiency of Rhodopseudomonas palustris strain PS3.

    Science.gov (United States)

    Lee, Sook-Kuan; Lur, Huu-Sheng; Lo, Kai-Jiun; Cheng, Kuan-Chen; Chuang, Chun-Chao; Tang, Shiueh-Jung; Yang, Zhi-Wei; Liu, Chi-Te

    2016-09-01

    Biofertilizers can help improve soil quality, promote crop growth, and sustain soil health. The photosynthetic bacterium Rhodopseudomonas palustris strain PS3 (hereafter, PS3), which was isolated from Taiwanese paddy soil, can not only exert beneficial effects on plant growth but also enhance the efficiency of nutrient uptake from applied fertilizer. To produce this elite microbial isolate for practical use, product development and formulation are needed to permit the maintenance of the high quality of the inoculant during storage. The aim of this study was to select a suitable formulation that improves the survival and maintains the beneficial effects of the PS3 inoculant. Six additives (alginate, polyethylene glycol [PEG], polyvinylpyrrolidone-40 [PVP], glycerol, glucose, and horticultural oil) were used in liquid-based formulations, and their capacities for maintaining PS3 cell viability during storage in low, medium, and high temperature ranges were evaluated. Horticultural oil (0.5 %) was chosen as a potential additive because it could maintain a relatively high population and conferred greater microbial vitality under various storage conditions. Furthermore, the growth-promoting effects exerted on Chinese cabbage by the formulated inoculants were significantly greater than those of the unformulated treatments. The fresh and dry weights of the shoots were significantly increased, by 10-27 and 22-40 %, respectively. Horticultural oil is considered a safe, low-cost, and easy-to-process material, and this formulation would facilitate the practical use of strain PS3 in agriculture.

  4. Signaling-State Formation Mechanism of a BLUF Protein PapB from the Purple Bacterium Rhodopseudomonas palustris Studied by Femtosecond Time-Resolved Absorption Spectroscopy.

    Science.gov (United States)

    Fujisawa, Tomotsumi; Takeuchi, Satoshi; Masuda, Shinji; Tahara, Tahei

    2014-12-26

    We studied the signaling-state formation of a BLUF (blue light using FAD) protein, PapB, from the purple bacterium Rhodopseudomonas palustris, using femtosecond time-resolved absorption spectroscopy. Upon photoexcitation of the dark state, FADH(•) (neutral flavin semiquinone FADH radical) was observed as the intermediate before the formation of the signaling state. The kinetic analysis based on singular value decomposition showed that FADH(•) mediates the signaling-state formation, showing that PapB is the second example of FADH(•)-mediated formation of the signaling state after Slr1694 (M. Gauden et al. Proc. Natl. Acad. Sci. U.S.A. 2006, 103, 10895-10900). The mechanism of the signaling-state formation is discussed on the basis of the comparison between femtosecond time-resolved absorption spectra of the dark state and those obtained by exciting the signaling state. FADH(•) was observed also with excitation of the signaling state, and surprisingly, the kinetics of FADH(•) was indistinguishable from the case of exciting the dark state. This result suggests that the hydrogen bond environment in the signaling state is realized before the formation of FADH(•) in the photocycle of PapB.

  5. Isolation of Rhp-PSP, a member of YER057c/YjgF/UK114 protein family with antiviral properties, from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b.

    Science.gov (United States)

    Su, Pin; Feng, Tuizi; Zhou, Xuguo; Zhang, Songbai; Zhang, Yu; Cheng, Ju'e; Luo, Yuanhua; Peng, Jing; Zhang, Zhuo; Lu, Xiangyang; Zhang, Deyong; Liu, Yong

    2015-11-04

    Rhodopseudomonas palustris strain JSC-3b isolated from a water canal adjacent to a vegetable field produces a protein that was purified by bioactivity-guided fractionation based on ammonium sulfate precipitation, ion-exchange absorption and size exclusion. The protein was further identified as an endoribonuclease L-PSP (Liver-Perchloric acid-soluble protein) by shotgun mass spectrometry analysis and gene identification, and it is member of YER057c/YjgF/UK114 protein family. Herein, this protein is designated Rhp-PSP. Rhp-PSP exhibited significant inhibitory activities against tobacco mosaic virus (TMV) in vivo and in vitro. To our knowledge, this represents the first report on the antiviral activity of a protein of the YER057c/YjgF/UK114 family and also the first antiviral protein isolated from R. palustris. Our research provides insight into the potential of photosynthetic bacterial resources in biological control of plant virus diseases and sustainable agriculture.

  6. Spectroscopic evidence for triplet excitation energy transfer among carotenoids in the LH2 complex from photosynthetic bacterium Rhodopseudomonas palustris

    Institute of Scientific and Technical Information of China (English)

    FENG Juan; WANG Qian; ZHANG Xujia; HUANG Youguo; AI Xicheng; ZHANG Xingkang; ZHANG Jianping

    2004-01-01

    The LH2 complex from Rhodopsudomonas (Rps.) palustris is unique in the heterogeneous carotenoid compositions. The dynamics of triplet excited state Carotenoids (3Car*) has been investigated by means of sub-microsecond time-resolved absorption spectroscopy both at physiological temperature (295 K) and at cryogenic temperature (77 K). Broad and asymmetric Tn←T1 transient absorption was observed at room temperature following the photo-excitation of Car at 532 nm, which suggests the contribution from various carotenoid compositions having different numbers of conjugated C=C double bonds (NC=C). The triplet absorption bands of different carotenoids, which superimposed at room temperature, could be clearly distinguished upon decreasing the temperature down to 77 K. At room temperature the shorter-wavelength side of the main Tn←T1 absorption band decayed rapidly to reach a spectral equilibration with a characteristic time constant of~1 μs, the same spectral dynamics, however, was not observed at 77 K. The aforementioned spectral dynamics can be explained in terms of the triplet-excitation transfer among heterogeneous carotenoid compositions. Global spectral analysis was applied to the time-resolved spectra at room temperature, which revealed two spectral components peaked at 545 and 565 nm, and assignable to the Tn←T1 absorption of Cars with NC=C=11 and NC=C=13, respectively. Surprisingly, the decay time constant of a shorter-con- jugated Car, I.e. 0.72 μs (aerobic) and 1.36 μs (anaerobic), is smaller than that of a longer-con- jugated Car, I.e. 2.12 μs (aerobic) and 3.75 μs (anaerobic), which is contradictory to the general rule of carotenoids and relative polyenes. The results are explained in terms of triplet-excitation transfer among different types of Cars. It is postulated that two Cars with different conjugation lengths coexist in an α,β-subunit in the LH2 complex.

  7. 沼泽红假单胞菌16S rDNA PCR快速检测方法研究%Study on the Detection Method of Rhodopseudomonas Palustris with 16S rDNA PCR

    Institute of Scientific and Technical Information of China (English)

    王妍力; 耿亚亚; 郝葆青

    2016-01-01

    Objective:according to the species attributes and highly conservation of 16S rDNA sequence of photosynthetic bacteria, the specific primers were designed, and the PCR reaction con-ditions were optimized, in order to explore the identifying methods to Rhodopseudomonas palustris. Method: the chromosomal DNA was extracted from Rhodopseudomonas palustris cell, and it was iso-lated and purified. The 16S rDNA gene sequences of the Pseudomonas genus, Escherichia coil and Nocardiopsis sp came from the GenBank respectively, and the gene sequence fragments of variable region were analyzed and compared with each other for the design of the species-specific primers. Several factors that may affect the PCR amplification were analyzed, and also some pre-tests were done in order to determine the best conditions for the PCR amplification. And then the PCR ampli-fication was done, the PCR products were cloned and sequenced. Meanwhile morphological and bio-chemical characteristics of Rhodopseudomonas palustris were tested. Conclusion:the method was de-veloped to detect Rhodopseudomonas palustris by PCR amplification with species-specific primers, which had the properties of strong specificity, high sensitivity, good repeatability, high maneuver-ability and low price. The whole process from extracting the DNA to the PCR amplification was about 3 h for identifying the strains of Rhodopseudomonas palustris.%目的:依据反映光合菌物种属性和高度保守性的16S rDNA序列,设计其特异性引物,优化并确定PCR反应条件,探索沼泽红假单胞菌快速鉴别的方法。方法:提取沼泽红假单胞菌菌体染色体DNA,分离和纯化;从GenBank分别获取假单胞菌属、大肠杆菌和诺卡氏菌属的16S rDNA基因序列,分析比较并确定其可变区的基因序列片段,设计种属特异引物;对影响PCR扩增的因素进行分析和预试,确定PCR扩增的最佳条件;随后进行PCR扩增试验,对其产

  8. Research Advance on the Mechanism of Anaerobic Catabolism of Aromatic Compounds by Rhodopseudomonas palustris%沼泽红甲单胞菌对芳香族化合物的厌氧降解机制研究进展

    Institute of Scientific and Technical Information of China (English)

    舒巧玉; 刘洋; 肖莉; 韩志萍; 叶金云

    2011-01-01

    The anaerobic catabolism of aromatic compounds by microorganism was very important for the sustainable development of biosphere and recycling of chemicals in environment. The paper summarized the mechanism of anaerobic catabolism of aromatic compounds by R. Palustris and the enzymes and genes related to it,and introduced the application of R. Palustris in degradation of aromatic compounds,which will provide reference for solving environmental pollution and biology energy study.%微生物厌氧降解芳香族化合物对于环境生化循环和生物圈的可持续发展具有重要作用.综述了沼泽红甲单胞菌(Rhodopseudomonas palustris)对芳香族化合物的厌氧降解机制、厌氧降解途径中的关键酶及其编码基因以及降解应用,为解决环境污染问题和生物能源研究提供了参考.

  9. Studies of interaction of homo-dimeric ferredoxin-NAD(P)+ oxidoreductases of Bacillus subtilis and Rhodopseudomonas palustris, that are closely related to thioredoxin reductases in amino acid sequence, with ferredoxins and pyridine nucleotide coenzymes.

    Science.gov (United States)

    Seo, Daisuke; Okabe, Seisuke; Yanase, Mitsuhiro; Kataoka, Kunishige; Sakurai, Takeshi

    2009-04-01

    Ferredoxin-NADP(+) oxidoreductases (FNRs) of Bacillus subtilis (YumC) and Rhodopseudomonas palustris CGA009 (RPA3954) belong to a novel homo-dimeric type of FNR with high amino acid sequence homology to NADPH-thioredoxin reductases. These FNRs were purified from expression constructs in Escherichia coli cells, and their steady-state reactions with [2Fe-2S] type ferredoxins (Fds) from spinach and R. palustris, [4Fe-4S] type Fd from B. subtilis, NAD(P)(+)/NAD(P)H and ferricyanide were studied. From the K(m) and k(cat) values for the diaphorase activity with ferricyanide, it is demonstrated that both FNRs are far more specific for NADPH than for NADH. The UV-visible spectral changes induced by NADP(+) and B. subtilis Fd indicated that both FNRs form a ternary complex with NADP(+) and Fd, and that each of the two ligands decreases the affinities of the others. The steady-state kinetics of NADPH-cytochrome c reduction activity of YumC is consistent with formation of a ternary complex of NADPH and Fd during catalysis. These results indicate that despite their low sequence homology to other FNRs, these enzymes possess high FNR activity but with measurable differences in affinity for different types of Fds as compared to other more conventional FNRs.

  10. 一株沼泽红假单胞菌对氮磷的同化能力%The Nitrogen and Phosphate Assimilating Capacity of a Rhodopseudomonas palustris Strain

    Institute of Scientific and Technical Information of China (English)

    刘敏; 梁运祥; 赵述淼; 谢贻天

    2012-01-01

    采用单因素和正交试验研究了不同营养成分对一株沼泽红假单胞菌氮磷同化能力的影响.结果表明,KH2PO4对沼泽红假单胞茵同化氮磷的能力有显著的影响.在同等发酵生物量的情况下,当NH4Cl为0.05 g/L、KH2PO4为1.40 g/L、酵母抽提物为1.00 g/L时,可使沼泽红假单胞茵的氮磷同化率分别由原来的32.51%和5.98%提高到48.02%和7.76%.%The effect of different nutriednt types on assimilative capacity to nitrogen and phosphate of a Rhodopseudomonas palustris strain was studied through single element and orthogonal experiments. The results showed that KH2PO4 was a notable factor affecting the assimilative capacity of this bacterium to nitrogen and phosphate. In condition of same fermented biomass, the assimilation efficiency of R. Palustris for nitrogen and phosphate could raise up to 48.02% and 7.76% instead of 32.51% and 5.98% respectively, when the content of NH4C1 was 0.05 g/L, KH2PO4 was 1.40 g/L and yeast extracts was 1.00 g/L.

  11. 光照厌氧条件下沼泽红假单胞菌对砷的抗性及其机制%Arsenic resistance mechanisms in Rhodopseudomonas palustris under anaerobic and light conditions

    Institute of Scientific and Technical Information of China (English)

    吕常江; 张永雨; 赵春贵; 郭少伟; 杨素萍; 陈少华

    2012-01-01

    采用UV-Vis和HPLC-ICP-MS分析方法,在光照厌氧条件下研究了沼泽红假单胞菌(Rhodopseudomonas palustris CQV97)对砷(As)的抗性和机制.结果表明,As(Ⅴ)与As(Ⅲ)对R.palustris CQV97的半数效应浓度(EC50)分别为2.3mmol·L-1和0.9mmol·L-1;该菌株能够将As(Ⅴ)还原为As(Ⅲ),不能将As(Ⅲ)转化为As(Ⅴ)或甲基砷;在含有0.1mmol·L-1As(Ⅴ)的培养基中培养80h,细胞积累的总As可达1.32mg·g-1(以干重计),其中,9.8%存在于细胞质中,4.9%与细胞膜的脂质相结合,其余被认为吸附在细胞表面;全细胞、细胞质、细胞膜所含的As(Ⅲ)和As(Ⅴ)的相对比例分别为16.3%和83.7%、12.1%和87.9%、16.5%和83.5%.静息细胞砷吸附结果表明,与0℃孵育细胞相比,25℃孵育细胞对As(Ⅴ)和As(Ⅲ)吸附量较高;灭活细胞对As(Ⅴ)的吸附量进一步提高,而对As(Ⅲ)的吸附量则降低.因此,光照厌氧条件下,R.palustrisCQV97对As具有较强的抗性和吸附特性,对As(Ⅴ)的抗性和吸附性均明显高于As(Ⅲ);其抗砷机制为细胞质As(Ⅴ)的还原途径,具体为在细胞内将As(Ⅴ)还原为As(Ⅲ),继而As(Ⅲ)被转运至细胞外,维持胞内As的含量在较低水平.本研究可为深入理解光合细菌对无机砷的抗性机制、砷的地球化学循环及砷的环境污染和生物修复提供理论参考.%The arsenic resistance mechanisms of a photosynthetic bacterium, Rhodopseudomonas palustris CQV97, under anaerobic and light conditions, were investigated in this study. It was revealed that the median effective concentration (EC50) of arsenite [As(Ⅲ)] and arsenate [As(Ⅴ)] for this bacterium were 0.9 mmol · L^-1 and 2.3 mmol · L^-1, respectively. In the cells of R. palustris CQV97, As(Ⅴ) could be reduced to As(Ⅲ), whereas As(Ⅲ) could not be transformed back to As(Ⅴ). Moreover, the cells lacked arsenic biomethylation ability. When R

  12. Effects of Vitamin B on Hydrogen Production and Sewage Treatment by Rhodopseudomonas palustris%B族维生素对沼泽红假单胞菌产氢以及降解废水的影响

    Institute of Scientific and Technical Information of China (English)

    傅春蓉; 赵红卫; 刘丽梅; 狄姗姗; 黄亮; 方为茂

    2011-01-01

    The research aimed to discuss the effects of Vitamin B on cell growth,Hydrogen productivity with simulated wastewater by Rhodopseudomonas palustris.The hydrogen production and wastewater treatment conditions were investigated using the basic medium supplemented with different kinds of vitamin B.The results showed that vitamin B has an impact on the cell growth of Rhodopseudomonas palustris,hydrogen productivity and simulated wastewater treatment capacity.The results based on the hydrogen production suggested that the contrast group,which has not any vitamin B,was the minimum volume of hydrogen,and the cumulative yield was 57.88mL/L.The results also showed that VB2 was beneficial to produce hydrogen,the yield was 83.25mL/L when lacking of VB2,while it was 128.83mL/L when adding all kinds of vitamin B.The yield of 337.43mL/L when lacking of VB4 showed that VB4 inhibited hydrogen production.As for the wastewater treatment,only the vitamin B complex has highest COD removal rate of 64.706%.%研究了B族维生素对沼泽红假单胞菌生长和产氢的影响以及其对模拟废水的处理效果。考察了在基础培养基中加入不同配比B族维生素时的产氢状况和废水处理效果。结果表明,B族维生素对沼泽红假单胞菌的生长,产氢以及处理废水能力都是有影响的。从产氢量的角度来看,空白对照组(未添加任何B族系列维生素)的产氢量最少,产氢量累积达到57.88 mL/L,加入全部维生素累积量128.83 mL/L,缺少VB2的产氢累积为83.25 mL/L,低于加入全部维生素累积量,因此VB2对产氢有促进作用,而缺少VB4的产氢量累积高达337.43 mL/L,高于加入全部维生素累积量,因此VB4对产氢有抑制作用。而对于废水处理效果来看,只加入复合VB的COD去除率达到最高值64.706%。

  13. 沼泽红假单胞菌的分离鉴定及对蕹菜生长的影响%Research on Isolation, Identification of Rhodopseudomonas palustris and Its Effects on the Growth of Ipomoea aquatica

    Institute of Scientific and Technical Information of China (English)

    黄小春; 王小东; 赵攀

    2014-01-01

    One strain of photosynthetic bacteria was enriched and isolated from soil amendment imported from Japan, named as GHR -507. The morphology and physiological -biochemical characteristics of this strain showed it belonged to Rhodopseudomonas palustris. With Ipomoea aquatica as the test material, and the leaves were sprayed by 500 times dilution of GHR-507 at young seedling stage, growth period, foliar application to spray water amount as controls. The plot yields were investigated in ripeness stage. The results showed that the production of first and second batch compared with antitheses increase 31.03%, 20.74%, respectively.%从引进的日本土壤改良剂中富集分离得到1株光合细菌,通过形态学观察及生理生化特性鉴定,鉴定为该菌株属于红假单胞菌属。以泰国柳叶蕹菜为试验材料,用GRH-507菌液稀释500倍在蕹菜幼苗期、生长期进行叶面喷施,以等量的清水作对照,在成熟期调查蕹菜的产量。结果表明,喷施500倍GRH-507菌液稀释液能提高蕹菜的产量,两次收割产量分别比对照增产31.03%、20.74%。

  14. Effect of dilution and L-malic acid addition on bio-hydrogen production with Rhodopseudomonas palustris from effluent of an acidogenic anaerobic reactor

    Energy Technology Data Exchange (ETDEWEB)

    Azbar, N.; Tuba, F.; Dokgoz, C. [Bioengineering Dept., Faculty of Engineering, Ege Univ., Izmir (Turkey)], E-mail: nuri.azbar@ege.edu.tr

    2009-07-01

    In this study, H{sub 2} was produced in a two-stage biological process: I) first stage; the dark fermentation of cheese whey wastewater, which is rich in lactose, by mixed anaerobic culture grown at thermophilic temperature in a continuously running fermentor and ii) second stage; the photo-fermentation of the residual medium by R. palustris strain (DSM 127) at 31{sup o}C under illumination of 150 W in batch mode, respectively. In the first part of the study, the effluent from the dark fermentation reactor was used either as it is (no dilution) or after dilution with distilled water at varying ratios such as 1/2 , 1/5, 1/10 (1 volume effluent/5 volume distilled water) before used in photo-fermentation experiments. In the second part of the study, L-malic acid at varying amounts was added into the hydrogen production medium in order to have L-malic acid concentrations ranging from 0 to 4 g/l. Non-diluted and pre-diluted mediums with or without L-malic acid addition were also tested for comparison purpose (as controls). Prior to the hydrogen production experiments, all samples were subjected to pH adjustment, (pH 6.7) and sterilized by autoclave at 121{sup o}C for 15 min. In regards to the experiments in which the effect of dilution of the effluent from dark fermentation was studied, it was observed that dilution of the effluent from dark fermentation resulted in much better hydrogen productions. Among the dilution rates used, the experiments operated with 1/5 dilution ratio produced the best hydrogen production (241 ml H{sub 2}/ g COD{sub fed}). On the other hand, it was seen that the mixing the effluent with L-malic acid (0 - 4 g/l) at increasing ratios (studied from 0% L-malic acid up to 100% by volume in the mixture) had further positive effect and improved the hydrogen production. The bioreactors containing only L-malic acid media resulted in the best hydrogen production (438 ml H{sub 2} / g COD{sub fed}). It was found that, undiluted raw cheese whey wastewater

  15. Biosynthesis of Silver Nanoparticles Using the Phototrophic Bacteria Rhodopseudomonas palustris and Its Antimicrobial Activity Against Escherichia coli and Staphylococcus aureus%沼泽红假单胞菌生物合成银纳米粒子及其抗菌作用

    Institute of Scientific and Technical Information of China (English)

    柴春镜; 白红娟

    2010-01-01

    近年来,利用沼泽红假单胞菌合成银纳米粒子作为一种可靠和环境友好的方法出现.主要利用沼泽红假单胞菌的细胞滤液来还原银离子.制备的纳米粒子用紫外可见光谱(UV-vis)、X射线衍射光谱(XRD)和透射电镜(TEM)进行表征.含有银粒子溶液的UV-vis光谱显示在420 nm-460 nm处出现银纳米粒子的吸收峰.TEM图像表明所形成的银纳米粒子的粒径范围为5 nm-20 nm.纳米粒子的XRD图谱证明产物为金属银.所制备的银纳米粒子对大肠杆菌和金黄色葡萄球菌作抑菌性试验.%The use of Rhodopseudomonas palustris in biosynthesis of silver nanoparticles (AgNPs) emerges as a reliable and eco-friendly approach in recent years. This report focuses on extracellular biosynthesis of AgNPs using cell filtrate of Rhodopseudomonas palustris. These nanoparticles were characterized by UV-vis spectrum, X-ray diffraction (XRD) spectrum and transmission electron microscopy (TEM). UV-vis spectrum of the aqueous medium containing silver ion showed a peak between 420 nm-460 nm corresponding to the plasmon absorbance of AgNPs. TEM micrograph showed formation of the AgNPs in the range of 5 nm-20 nm. XRD of the nanoparticles confirmed the formation of metallic silver. The AgNPs were evaluated for their antimicrobial activities against Escherichia coli and Staphyloccocus aureus.

  16. Denitrifying Characterizations from Inorganic Nitrogen-polluted Wastewater by Rhodopseudomonas palustris CQV97%沼泽红假单胞菌CQV97菌株对污染水体三氮去除特性研究

    Institute of Scientific and Technical Information of China (English)

    赵玥; 赵春贵; 陈龑; 杨素萍

    2012-01-01

    在不同污染程度模拟水体中,利用沼泽红假单胞菌CQV97,在厌氧光照条件下,研究了水体中氨氯、硝态氮和亚硝态氮含量、菌体生物量和水体pH的变化关系.随时间延长,CQV97菌株对氨氮、硝态氮或亚硝态氮去除量增大,生物量增加,水体pH升高;随氨氮浓度提高,生物量增加,氨氮低于33.2 mg/L能被完全去除,最大去除量达84.2 mg/L,水体pH维持在9.2~9.4;随硝态氮浓度的升高,菌体生物量降低,浓度低于216.96 mg/L能被完全去除,pH维持在9.1~9.3.随亚硝态氮浓度增加,菌体生长延滞期延长,生物量和pH升高幅度降低,浓度低于128.2mg/L能被完全去除.结果表明,CQV97菌株对氨氮、硝氮和亚硝氮具有良好的去除能力.%The relationship among removal amount and removal rate of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen,biomass as well as pH change in simulation wastewater polluted by different nitrogen compounds with Rhodopseudomonas palustris CQV97 were investigated under anaerobically in the light. The results showed that strain CQV 97 had better capability of ammonia nitrogen removal. The removal a-mount and removal rate of ammonia nitrogen, biomass and pH increased during 10 d. With the increasing ammonia nitrogen concentration, the removal amount of ammonia nitrogen and biomass enhanced, pH reached at 9. 2~9. 4,but the apparent removal rate of ammonia nitrogen reduced. Less than 33. 2 mg/L ammonia nitrogen could be completely removed. The maximal removal amount of ammonia nitrogen reached 84.2 mg/L. In the nitrate nitrogen-containing polluted wastewater, strain CQV97 could utilize, transform and rapidly and effectively remove nitrate nitrogen, cell growth and pH gradually increased while the nitrate nitrogen was constantly removed. With increasing nitrate nitrogen concentration, the biomass decreased, pH maintained at 9. 1~9. 3. The nitrate nitrogen could be removed completely and the maximal removal

  17. Hybrid Culture on Impact of Hydrogen Production and Metabolism of Rhodopseudomonas palustris%混合培养对沼泽红假单孢菌产氢及代谢的影响

    Institute of Scientific and Technical Information of China (English)

    叶姜瑜; 刘婧; 廖强

    2009-01-01

    混合培养逐渐引起人们的关注,将沼泽红假单孢菌Rhodopseudomonas palustris和厌氧细菌Enterobacter aerogenes PCM 532的混合培养,并进行生长代谢、产氢及正交实验、Biolog碳源代谢实验.结果表明,混合菌在厌氧光照条件下生长迅速,在好氧黑暗条件下也有一定生长;混合培养的产氢量高于纯培养;正交实验得到,对混合培养产氖影响最大的是底物和混合比例;Biolog微孔板代谢中混合菌可以利用纯菌种培养不能利用的碳源.混合培养表现为协同作用,促进细菌生长和提高产氢能力.

  18. 芳香族氨基酸对沼泽红假单胞菌合成CoQ10的影响%Effects of aromatic amino acids on the biosynthesis of CoQ10 in Rhodopseudomonas palustris

    Institute of Scientific and Technical Information of China (English)

    蒋世云; 余龙江; 申晓林; 熊欣; 简艳

    2008-01-01

    CoQ10 has been used not only as a drug but also as a food supplement because of its various Dhysi ological and biochemical activities.Microbial fermentation is a main way for the commercial production of CoQ10.The quinoid nucleus of CoQ10 and aromatic amino acids are synthesized from the shikimate pathway in bacteria and there are some effects of them on another biosynthesis.The impacts of aromatic amino acids on the biosynthesis of CoQ10 in Rhodopseudomnas palustris J001 were investigated with feeding aromatic amino acids at the end of the logarithmic phase during incubation.The results showed that the feeding of tryptophan(above 15 mg/L) strongly repressed the biosynthesis of CoQ10.There were also some slightly unfavorable effects of feeding of phenylalanine(50 mg/L)and tyrosine(above 200 rag/L)on the biosynthesis of CoQl0.However,the biosynthe sis of CoQ10 could be enhanced by feeding phenylalanine(above 75 mg/L)Or tyrosine(from 75 to 1 75 mg/L).The content of CoQ10 in the strain was found to be the highest up tO(25.6±1.3)mg CoQ10/g dried biomass when feeding phenylalanine(1 00 mg/L)+tyrosine(1 50 mg/L),which was 52.2% higher than that in the control with no additional amino acids in the medium.The results indicated that the biosynthesis of CoqIo was affected by the three aromatic amino acids.It can be concluded that the biosynthesis of CoQ10 in strain is favorable by feeding appropriate amount of phenylalanien and tyrosine without tryptophan.%CoQ10具有呼吸链电子传递者、抗氧化性、调控基因表达等多种生理生化功能.目前不仅用作药物也用作食品添加剂.微生物发酵法是当前生产CoQ10的主要方法.在细菌中,芳香族氨基酸和CoQ10的苯核环都是通过莽草酸途径合成;它们在生物体中各自的合成存在着相互调控作用.本试验通过在培养过程中添加芳香族氨基酸来考察了其对沼泽红假单胞菌J001合成CoQ10的影响.结果表明:当色氨酸添加量≥15 mg/L时对CoQ10

  19. Effizienzsteigerung durch intelligente Planung

    Science.gov (United States)

    Maier, Walther; Bader, Alexander

    Das Umfeld der produzierenden Unternehmen hat sich in den letzten Jahren sehr gewandelt. Dieser Wandel ist geprägt durch die Folgen der Globalisierung und deren Folgeerscheinungen. Produzierende Unternehmen versuchen auf diese geänderten Rahmenbedingungen durch Steigerung ihrer Produktivität und Flexibilität zu reagieren.

  20. Chemische Analyse durch Spectralbeobachtungen

    OpenAIRE

    Kirchhoff, Gustav Robert; Bunsen, Robert Wilhelm

    2008-01-01

    1860 fanden Gustav R. Kirchhoff und Robert W. Bunsen in ihrem Heidelberger Labor heraus, dass das durch ein Prisma geleitete Licht erhitzter chemischer Elemente charakterische Linien erzeugt. Diese Linien erlauben die eindeutige Fernidentifizierung der beteiligten Stoffe. Die beiden Forscher publizierten ihre Entdeckung in den Annalen der Physik und Chemie.

  1. Manifestations of native topology in the denatured state ensemble of Rhodopseudomonas palustris cytochrome c'.

    Science.gov (United States)

    Dar, Tanveer A; Schaeffer, R Dustin; Daggett, Valerie; Bowler, Bruce E

    2011-02-15

    To provide insight into the role of local sequence in the nonrandom coil behavior of the denatured state, we have extended our measurements of histidine-heme loop formation equilibria for cytochrome c' to 6 M guanidine hydrochloride. We observe that there is some reduction in the scatter about the best fit line of loop stability versus loop size data in 6 M versus 3 M guanidine hydrochloride, but the scatter is not eliminated. The scaling exponent, ν(3), of 2.5 ± 0.2 is also similar to that found previously in 3 M guanidine hydrochloride (2.6 ± 0.3). Rates of histidine-heme loop breakage in the denatured state of cytochrome c' show that some histidine-heme loops are significantly more persistent than others at both 3 and 6 M guanidine hydrochloride. Rates of histidine-heme loop formation more closely approximate random coil behavior. This observation indicates that heterogeneity in the denatured state ensemble results mainly from contact persistence. When mapped onto the structure of cytochrome c', the histidine-heme loops with slow breakage rates coincide with chain reversals between helices 1 and 2 and between helices 2 and 3. Molecular dynamics simulations of the unfolding of cytochrome c' at 498 K show that these reverse turns persist in the unfolded state. Thus, these portions of the primary structure of cytochrome c' set up the topology of cytochrome c' in the denatured state, predisposing the protein to fold efficiently to its native structure.

  2. Blutdrucksenkung durch Entspannung

    OpenAIRE

    Wertgen T

    2014-01-01

    Unser Lebensstil ist geprägt durch Informationsflut, permanente Erreichbarkeit und Bewegungsarmut. Stresssymptome und gleichzeitig hohe Blutdruckwerte finden wir bei vielen Patienten. In diesem Artikel werden das Achtsamkeitstraining, das Biofeedback-Kohärenztraining, die kontemplative Meditation und die medizinische Hypnose als mögliche therapeutische Verfahren bei arterieller Hypertonie besprochen. Der Einsatz dieser Methoden bei Patienten, die unter unerwünschten Wirkungen bei medikament...

  3. Blutdrucksenkung durch Entspannung

    Directory of Open Access Journals (Sweden)

    Wertgen T

    2014-01-01

    Full Text Available Unser Lebensstil ist geprägt durch Informationsflut, permanente Erreichbarkeit und Bewegungsarmut. Stresssymptome und gleichzeitig hohe Blutdruckwerte finden wir bei vielen Patienten. In diesem Artikel werden das Achtsamkeitstraining, das Biofeedback-Kohärenztraining, die kontemplative Meditation und die medizinische Hypnose als mögliche therapeutische Verfahren bei arterieller Hypertonie besprochen. Der Einsatz dieser Methoden bei Patienten, die unter unerwünschten Wirkungen bei medikamentöser antihypertensiver Therapie leiden, bei frühem Hypertoniestadium, hoher Motivation oder hohem Stresslevel ist insbesondere in Kombination sinnvoll.

  4. Spontaneous Nif- mutants of Rhodopseudomonas capsulata.

    OpenAIRE

    Wall, J D; Love, J.; Quinn, S P

    1984-01-01

    Revertible, spontaneous Nif- mutants of Rhodopseudomonas capsulata have been shown to accumulate in cultures growing photosynthetically with an amino acid as the nitrogen source such that H2 is maximally produced. The majority of such strains carry mutations which are clustered in a short region of the chromosome, probably representing one or two genes. Because this cluster includes temperature-sensitive mutations, it is also likely that it identifies the structural gene of a polypeptide. The...

  5. Small RNAs of the Bradyrhizobium/Rhodopseudomonas lineage and their analysis.

    Science.gov (United States)

    Madhugiri, Ramakanth; Pessi, Gabriella; Voss, Björn; Hahn, Julia; Sharma, Cynthia M; Reinhardt, Richard; Vogel, Jörg; Hess, Wolfgang R; Fischer, Hans-Martin; Evguenieva-Hackenberg, Elena

    2012-01-01

    Small RNAs (sRNAs) play a pivotal role in bacterial gene regulation. However, the sRNAs of the vast majority of bacteria with sequenced genomes still remain unknown since sRNA genes are usually difficult to recognize and thus not annotated. Here, expression of seven sRNAs (BjrC2a, BjrC2b, BjrC2c, BjrC68, BjrC80, BjrC174 and BjrC1505) predicted by genome comparison of Bradyrhizobium and Rhodopseudomonas members, was verified by RNA gel blot hybridization, microarray and deep sequencing analyses of RNA from the soybean symbiont Bradyrhizobium japonicum USDA 110. BjrC2a, BjrC2b and BjrC2c belong to the RNA family RF00519, while the other sRNAs are novel. For some of the sRNAs we observed expression differences between free-living bacteria and bacteroids in root nodules. The amount of BjrC1505 was decreased in nodules. By contrast, the amount of BjrC2a, BjrC68, BjrC80, BjrC174 and the previously described 6S RNA was increased in nodules, and accumulation of truncated forms of these sRNAs was observed. Comparative genomics and deep sequencing suggest that BjrC2a is an antisense RNA regulating the expression of inositol-monophosphatase. The analyzed sRNAs show a different degree of conservation in Rhizobiales, and expression of homologs of BjrC2, BjrC68, BjrC1505, and 6S RNA was confirmed in the free-living purple bacterium Rhodopseudomonas palustris 5D.

  6. Durch das Leben lernen

    Directory of Open Access Journals (Sweden)

    Ingolf Waßmann

    2014-12-01

    Full Text Available Web 2.0 und soziale Netzwerke gaben erste Impulse für neue Formen der Online-Lehre, welche die umfassende Vernetzung von Objekten und Nutzern im Internet nachhaltig einsetzen. Die Vielfältigkeit der unterschiedlichen Systeme erschwert aber deren ganzheitliche Nutzung in einem umfassenden Lernszenario, das den Anforderungen der modernen Informationsgesellschaft genügt. In diesem Beitrag wird eine auf dem Konnektivismus basierende Plattform für die Online-Lehre namens “Wiki-Learnia” präsentiert, welche alle wesentlichen Abschnitte des lebenslangen Lernens abbildet. Unter Einsatz zeitgemäßer Technologien werden nicht nur Nutzer untereinander verbunden, sondern auch Nutzer mit dedizierten Inhalten sowie ggf. zugehörigen Autoren und/oder Tutoren verknüpft. Für ersteres werden verschiedene Kommunikations-Werkzeuge des Web 2.0 (soziale Netzwerke, Chats, Foren etc. eingesetzt. Letzteres fußt auf dem sogenannten “Learning-Hub”-Ansatz, welcher mit Hilfe von Web-3.0-Mechanismen insbesondere durch eine semantische Metasuchmaschine instrumentiert wird. Zum Aufzeigen der praktischen Relevanz des Ansatzes wird das mediengestützte Juniorstudium der Universität Rostock vorgestellt, ein Projekt, das Schüler der Abiturstufe aufs Studium vorbereitet. Anhand der speziellen Anforderungen dieses Vorhabens werden der enorme Funktionsumfang und die große Flexibilität von Wiki-Learnia demonstriert.

  7. Radiosensibilisierung durch BRAF Inhibitoren.

    Science.gov (United States)

    Strobel, Sophia Boyoung; Pätzold, Sylvie; Zimmer, Lisa; Jensen, Alexandra; Enk, Alexander; Hassel, Jessica Cecile

    2017-07-01

    In der letzten Zeit wurden in der Literatur vermehrt erhöhte Hauttoxizitäten während einer Kombinationstherapie mit BRAF Inhibitoren und Radiotherapie beschrieben. Wir berichten über sieben Melanompatienten in einem nicht resezierbaren Stadium III oder IV, die eine kombinierte Behandlung aus Bestrahlung und BRAF-Inhibitor erhielten. Bei allen Patienten konnte durch die Kombinationstherapie ein gutes lokales Ansprechen erreicht werden. Nur bei zwei Patienten wurde eine schwere Radiodermatitis (CTCAE Grad 3 bzw. 4) beobachtet. Bei diesen Patienten, die beide Vemurafenib erhielten, trat die Radiodermatitis nach ein bzw. zwei Wochen auf und resultierte in einer Unterbrechung der BRAF-Inhibitor Behandlung.. Die kumulative Dosis bis zum Zeitpunkt der Strahlendermatitis betrug 10 Gy bzw. 35 Gy. Bei allen anderen Vemurafenibpatienten konnten nur milde Reaktionen im Sinne einer Radiodermatitis CTCAE Grad 2, beim Dabrafenibpatienten CTCAE Grade 1 diagnostiziert werden. Bei einem Patienten wurde eine Recalldermatitis nach 14 Tagen einer beendeten Strahlentherapie mit einer kumulativen Dosis von 30 Gy diagnostiziert. Schwere Toxizitätsreaktionen der Haut unter einer BRAF-Inhibitionen treten nicht häufig auf und sind meistens gut therapierbar. Deshalb sollte die Kombinationstherapie bei aggressiv wachsenden Melanomen eine Therapieoption bleiben. Obwohl ein erhöhtes Risiko der Hauttoxizität unter einer Kombinationstherapie von Radiatio und BRAF-Inhibitoren besteht, wird diese von den meisten Patienten gut toleriert. Sequenzielle Therapie anstelle von gleichzeitiger Behandlung scheint die Toxizitätreaktionen nicht zu verhindern. © 2017 Deutsche Dermatologische Gesellschaft (DDG). Published by John Wiley & Sons Ltd.

  8. Single-molecule spectroscopy on RC-LH1 complexes of Rhodopseudomonas acidophila strain 10050.

    Science.gov (United States)

    Böhm, Paul S; Southall, June; Cogdell, Richard J; Köhler, Jürgen

    2013-03-21

    We have revisited the RC-LH1 complex from Rhodopseudomonas (Rps.) acidophila for single-molecule spectroscopy. For the current study the pigment-protein complexes were stabilized in the detergent buffer solution using a relatively mild detergent (dodecyl-β-D-maltoside (DDM) instead of lauryldimethylamine N-oxide (LDAO)). This leads to a significant reduction of the fraction of broken/dissociated RC-LH1 complexes with respect to previous studies and has allowed us to investigate a sufficiently large sample of individual RC-LH1 complexes. For most of the complexes the fluorescence-excitation spectra exhibit a narrow spectral feature at the red end of the spectrum. Analysis of the statistics of the spectral properties yields a close resemblance with the results obtained on RC-LH1 complexes from Rps. palustris for which a low-resolution X-ray structure is available. Based on this comparison we come to the conclusion that for both species the RC-LH1 complex can be described by the same structural model, that is, an overall elliptical assembly of pigments that features a gap.

  9. Irritation durch Waschen und Desinfizieren

    OpenAIRE

    Weimer, Caroline Maria

    2006-01-01

    Ziel dieser Studie war die Irritation der Haut, hervorgerufen durch alkoholische Desinfektionsmittel und das Detergens Natriumlaurylsulfat (0,5% NLS) in einem repetitiven Testdesign zu untersuchen. Mittels nicht invasiver Untersuchungsmethoden quantifizierten wir die irritativen Effekte von Sterillium®, 2-Propanol 45% v/v, 1-Propanol 30% v/v, welches die alkoholische Grundlage von Sterillium® darstellt sowie von Wasser und NLS 0,5...

  10. Hydrogen production by Rhodopseudomonas palustris WP 3-5 in a serial photobioreactor fed with hydrogen fermentation effluent.

    Science.gov (United States)

    Lee, Chi-Mei; Hung, Guo-Jan; Yang, Chu-Fang

    2011-09-01

    In this study, a lab-scale serial photobioreactor composed of three column reactors was constructed and continuously operated to investigate several parameters influencing photohydrogen production when using the synthetic wastewater and the anaerobic hydrogen fermentation effluents as the influents. The results indicated that better hydrogen production rate was obtained when the serial photobioreactor was operated under cellular recycling at a short HRT of 8h. The serial photobioreactor maintained high hydrogen content ca. 80% in the produced gas and 0.4× dilution ratio was the suitable ratio for hydrogen production. When the photobioreactor fed with the real wastewater (Effluent 1) containing 100 mg/L NH4Cl, Column 1 reactor successfully reduced ammonia concentration to about 60 mg/L for cell synthesis, resulting in a steady hydrogen production in the following two column reactors. The average hydrogen production rate was 205 mL-H2/L/d.

  11. Genome sequence of Kocuria palustris strain W4

    DEFF Research Database (Denmark)

    Herschend, Jakob; Raghupathi, Prem Krishnan; Røder, Henriette Lyng;

    2016-01-01

    We report the 3.09 Mb draft genome sequence ofKocuria palustrisW4, isolated from a slaughterhouse in Denmark.......We report the 3.09 Mb draft genome sequence ofKocuria palustrisW4, isolated from a slaughterhouse in Denmark....

  12. Genome sequence of Kocuria palustris strain W4

    DEFF Research Database (Denmark)

    Herschend, Jakob; Raghupathi, Prem Krishnan; Røder, Henriette Lyng

    2016-01-01

    We report the 3.09 Mb draft genome sequence ofKocuria palustrisW4, isolated from a slaughterhouse in Denmark.......We report the 3.09 Mb draft genome sequence ofKocuria palustrisW4, isolated from a slaughterhouse in Denmark....

  13. Complete Genome Sequence of Kocuria palustris MU14/1.

    Science.gov (United States)

    Calcutt, Michael J; Foecking, Mark F

    2015-10-15

    Presented here is the first completely assembled genome sequence of Kocuria palustris, an actinobacterial species with broad ecological distribution. The single, circular chromosome of K. palustris MU14/1 comprises 2,854,447 bp, has a G+C content of 70.5%, and contains a deduced gene set of 2,521 coding sequences.

  14. Complete Genome Sequence of Kocuria palustris MU14/1

    OpenAIRE

    2015-01-01

    Presented here is the first completely assembled genome sequence of Kocuria palustris, an actinobacterial species with broad ecological distribution. The single, circular chromosome of K. palustris MU14/1 comprises 2,854,447 bp, has a G+C content of 70.5%, and contains a deduced gene set of 2,521 coding sequences.

  15. Krank durch Sport: Anorexia athletica

    Directory of Open Access Journals (Sweden)

    Friedmann-Bette B

    2012-01-01

    Full Text Available Sportler aus Sportarten, in denen ein geringes Körpergewicht vorteilhaft für die Leistungsfähigkeit ist, gehen oft durch Reduktion der Kalorienzufuhr bei gleichzeitig hohem Energieverbrauch ein erhebliches Energiedefizit ein. Dabei kommt es häufig zu Essstörungen, für die in besonders gefährdeten Sportarten eine Prävalenz von bis zu 80 % berichtet wird und die sich zur Anorexia nervosa oder Bulimia nervosa entwickeln können. Als Folge des Energiedefizits können Konzentrationsänderungen von Stoffwechselhormonen, Störungen der Hypothalamus- Hypophysen-Gonaden-Achse und des Knochenstoffwechsels mit Abnahme der Knochendichte bis hin zur Osteoporose und einem erhöhten Risiko für Stressfrakturen beobachtet werden. Der Symptomkomplex aus Essstörung, sekundärer Amenorrhö und verminderter Knochendichte wird auch als „Female Athlete Triad“ bezeichnet, wobei in den vergangenen Jahren als viertes Symptom eine endotheliale Dysfunktion beschrieben wurde. Die effektivste Therapie ist das Herstellen eines dauerhaften Energiegleichgewichts, wofür eine tägliche Energieverfügbarkeit von mindestens 30 kcal/kg Magergewicht erforderlich ist. Eine hierdurch bedingte Gewichtszunahme kann den Sportlern nicht immer vermittelt werden, weshalb durch eine sorgfältig zusammengestellte Ernährung zumindest auf eine ausreichende Zufuhr von Kalzium, Vitamin D und Eiweiß geachtet werden muss. Oftmals ist die zusätzliche Verabreichung von Kalzium- und Vitamin-D-Präparaten angezeigt sowie eine Östrogensubstitution. Allerdings ist ein orales Kontrazeptivum als einzige Therapie – anders als früher angenommen – nicht ausreichend zur Behandlung oder Vermeidung einer Knochendichteminderung. Zur Prävention der Anorexia athletica sind die Aufklärung der Sportler, Trainer und Betreuer und ein Screening im Rahmen von Sporttauglichkeitsuntersuchungen, vor allem aber auch strukturelle Maßnahmen durch die Sportfachverbände erforderlich (wie zum Beispiel im

  16. The Photosynthetic Reaction Center from the Purple Bacterium Rhodopseudomonas viridis

    Science.gov (United States)

    Deisenhofer, Johann; Michel, Hartmut

    1989-09-01

    The history and methods of membrane protein crystallization are described. The solution of the structure of the photosynthetic reaction center from the bacterium Rhodopseudomonas viridis is described, and the structure of this membrane protein complex is correlated with its function as a light-driven electron pump across the photosynthetic membrane. Conclusions about the structure of the photosystem II reaction center from plants are drawn, and aspects of membrane protein structure are discussed.

  17. Aberrante Epigynenbildungen bei der Wolfspinne Pardosa palustris (Araneae, Lycosidae

    Directory of Open Access Journals (Sweden)

    Martin, Dieter

    2013-11-01

    Full Text Available Two cases of aberrant epigyne shape in Pardosa palustris (Linnaeus, 1758 are described. Characteristic is the absence of the posterior lateral parts of the septum. Possible causes, such as `genital damage` during mating or the effects of parasite infestation, are discussed.

  18. Variability of Caltha palustris L. populations in garden culture

    Directory of Open Access Journals (Sweden)

    Krystyna Falińska

    2014-01-01

    Full Text Available On the basis of studies performed in the experimental garden the character of the variability of Caltha palustris L. populations is described. Individuals were bred under uniform conditions from diaspores of meadow, springwood, flood-plain forest and alder forest populations. The results obtained allow to evaluate the hypothesis concerning the ecological preference of cytotypes (S m i t 1967, 1968 and the somewhat different ecological requirements of two subspecies: C. palustris ssp. palustris and C. palustris ssp. cornuta. It was found that each population includes individuals with different cytotypes. The situation is similar as far as subspecies are concerned, distinguished on the basis of fruit morphology (Fig. 1. It should be stressed, however, that, investigations of many years duration raised serious doubts as to the diagnostic value of fruit morphology (Figs. 2, 3. On the basis of the preserved differences between the populations in shoot habitus, reproduction and phenology in garden culture, a springwood and an alder forest ecotype were distinguished. Meadow and flood-plain populations exhibited a transitional character with certain similarities both to the alder forest and to the springwood populations.

  19. Understory plant biomass dynamics of prescribed burned Pinus palustris stands

    Science.gov (United States)

    C.A. Gonzalez-Benecke; L.J. Samuelson; T.A. Stokes; W.P. Cropper Jr; T.A. Martin; K.H. Johnsen

    2015-01-01

    Longleaf pine (Pinus palustris Mill.) forests are characterized by unusually high understory plant species diversity, but models describing understory ground cover biomass, and hence fuel load dynamics, are scarce for this fire-dependent ecosystem. Only coarse scale estimates, being restricted on accuracy and geographical extrapolation,...

  20. Decolorization of Anthraquinone dye by Rhodopseudomonas XL-1

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Rhodopseudomonas XL-1 gained from textile wastewater can effectively decolorize anthraquinone dye. Under anaerobic condition, 93 percent of the anthraquinone dye is decolorized , which is higher than that under aerobic condition. The optimum pH is 6~9 and the optimum temperature is 20~40℃ for the anthraquinone dye decolorization by XL-1 . XL-1 can not decolorize the anthraquinone dye when it is the sole carbon source. Microbial cometabolism and decolorization of the dye take place in the presence of some other carbon source(0.2~0.4g/100ml)called cometabolic substrate. The cometabolic substrate can be peptone, glucose, sodium acetate, beef extract, amylum, etc. The change of molecular structure of the dye before and after decolorized by XL-1 is studied by UV-Vis absorption spectrum. The results indicate that its molecular structure is changed evidently.

  1. Beschichtung von Kohlenstofffasern durch Chemische Gasphasenabscheidung (CVD)

    OpenAIRE

    Schmidt, Stephan

    2005-01-01

    Der Einsatz faserverstärkter Verbundwerkstoffe mit metallischer und keramischer Matrix bietet den Vorteil von Kombination und optimaler Ausnutzung verschiedenster Werkstoffeigenschaften. Die Verwendung einer durch Kohlenstofffasern verstärkten keramischen Matrix erlangte dabei in der kürzeren Vergangenheit vermehrtes Interesse. Grundlegend für den erfolgreichen Einsatz von Faserverbundwerkstoffen ist dabei ein optimiertes Faser-Matrix-Interface, also eine "optimale" Haftung der Fasern innerha...

  2. Erweiterung modellbasierter Segmentierung durch lokale Deformationskriterien

    Science.gov (United States)

    Erdt, Marius; Tulchiner, Roman; Sakas, Georgios

    Modellbasierte Ansätze sind heutzutage Stand der Technik zur automatischen Organsegmentierung in medizinischen Bilddatensätzen. In dieser Arbeit wird ein Verfahren vorgestellt, welches die modellbasierte Segmentierung durch lokale Deformationskriterien erweitert, um eine bessere lokale Anpassung der Oberflächenmodelle an Bildstrukturen sowohl hoher als auch niedriger Frequenz zu erreichen. Die beschriebene Methode wird anhand von Computer-Tomographie Datensätzen der Niere beschrieben und evaluiert.

  3. Stabilisierung von pharmazeutischen Proteinlösungen durch Vakuumtrocknung

    OpenAIRE

    2003-01-01

    Das Ziel der vorliegenden Arbeit war es, verschiedene Vakuumtrocknungs-Verfahren für die Trocknung von Proteinen verfahrenstechnisch zu optimieren. Dies geschah zum einen durch die Verwendung unterschiedlicher Geräte und zum anderen durch den Einsatz spezieller Rezepturmodifizierungen. Dabei war es notwendig, das Verhalten der Proteine während des Trocknungsprozesses durch zahlreiche Versuchsansätze und mit mehreren analytischen Methoden zu charakterisieren. Das bei der Trocknung auftretende ...

  4. Einsparungspotential bei Antihypertensiva durch integriertes, ambulantes Hypertonikertraining

    Directory of Open Access Journals (Sweden)

    Kurz RW

    2001-01-01

    Full Text Available Die arterielle Hypertonie stellt unverändert einen der wichtigsten Risikofaktoren für Herzkreislauferkrankungen dar. Aufgrund der hohen Prävalenz und der zumeist lebenslangen Therapieindikation verursacht die antihypertensive Behandlung hohe Kosten für das Gesundheitswesen. Für verschiedene nicht-pharmakologische Maßnahmen konnte ein blutdrucksenkender Effekt nachgewiesen werden. In einem Versuch, einige dieser Therapiemodalitäten zu kombinieren, wurde am Zentrum für Ambulante Rehabilitation ein integriertes, ambulantes Trainingsprogramm erstellt und wird hypertensiven Patienten angeboten. Dieses Programm kombiniert Bewegungstherapie, Patientenschulung, Entspannungstechniken und Streßmanagement. Bei Bedarf wird individuell eine Psychotherapie angeboten. Die Effektivität des vorgestellten Programms kann durch eine gesteigerte körperliche Leistungsfähigkeit und eine verbesserte Blutdruckregulation trotz signifikanter Reduktion des Medikamentenbedarfs unter Beweis gestellt werden. Gegenwärtig stellen die stark steigenden Medikamentenkosten für die Allgemeinheit Anlaß zur Sorge dar und haben eine teilweise emotional geführte Diskussion um die zukünftige Entwicklung des Gesundheitssystems entfacht. In einer Kosten-Nutzen-Analyse haben wir im weiteren versucht, die Kosten für das beschriebene Trainingsprogramm zu erfassen und haben diese den potentiellen Einsparungen durch reduzierten Medikamentenverbrauch gegenübergestellt. Die vorliegende Arbeit präsentiert eine Zwischenauswertung einer derzeit laufenden Untersuchung und gibt eine Übersicht über die relevante Literatur zum Thema der nicht-phamakologischen Therapie der Hypertonie.

  5. Effects of the Medium Composition on the Components of the Electrochemical Proton Gradient in Rhodopseudomonas sphaeroides

    NARCIS (Netherlands)

    Michels, Paul A.M.; Hellingwerf, K; Lolkema, Juke S.; Friedberg, Ilan; Konings, Wilhelmus

    1981-01-01

    The magnitude and composition of the proton motive force (Δµ~H+) has been measured in chromatophores and whole cells of Rhodopseudomonas sphaeroides as a function of the ionic composition of the buffer in which the energy-transducing membranes are suspended. Measurements with the flow-dialysis techn

  6. Energy Coupling of Facilitated Transport of Inorganic Ions in Rhodopseudomonas sphaeroides

    NARCIS (Netherlands)

    Hellingwerf, K; Friedberg, Ilan; Lolkema, Juke S.; Michels, Paul A.M.; Konings, Wilhelmus

    1982-01-01

    Within the scope of a study on the effects of changes in medium composition on the proton motive force in Rhodopseudomonas sphaeroides, the energy coupling of sodium, phosphate, and potassium (rubidium) transport was investigated. Sodium was transported via an electroneutral exchange system against

  7. Effects of the Medium Composition on the Components of the Electrochemical Proton Gradient in Rhodopseudomonas sphaeroides

    NARCIS (Netherlands)

    Michels, Paul A.M.; Hellingwerf, K; Lolkema, Juke S.; Friedberg, Ilan; Konings, Wilhelmus

    1981-01-01

    The magnitude and composition of the proton motive force (Δµ~H+) has been measured in chromatophores and whole cells of Rhodopseudomonas sphaeroides as a function of the ionic composition of the buffer in which the energy-transducing membranes are suspended. Measurements with the flow-dialysis techn

  8. Review Essay: Eine Reise durch die Grounded Theory

    OpenAIRE

    2007-01-01

    In "Constructing Grounded Theory" begleitet Kathy CHARMAZ die Leser(innen) durch den Forschungsprozess. Ausgehend von einem historischen Rückblick auf die Entwicklung der Grounded Theory Methodologie führt sie die Lesenden von der Datenerfassung über das Kodieren zum Schreiben von Memos und schließlich zum ersten Entwurf der eigenen Grounded Theory. Durch die zahlreichen Beispiele aus ihrer eigenen Forschung erhalten Lesende jedoch mehr als einen theoretischen Überblick; sie haben vielmehr di...

  9. Genetic Mapping of the Rhodopseudomonas capsulata Chromosome Shows Non-clustering of Genes Involved in Nitrogen Fixation

    National Research Council Canada - National Science Library

    WILLISON, JOHN C; AHOMBO, GABRIEL; CHABERT, JACQUELINE; MAGNIN, JEAN-PIERRE; VIGNAIS, PAULETTE M

    1985-01-01

    ... Nucléaires de Grenoble, 85 X, 38041 Grenoble Cedex, France ABSTRACT Summary: The mutant R plasmid pTH10 was used to construct a circular linkage map of the Rhodopseudomonas capsulata B10 chromosome...

  10. Reproductive strategies of Caltha palustris L. under various living conditions

    Directory of Open Access Journals (Sweden)

    Cezary Werpachowski

    2014-01-01

    Full Text Available Reproductive effort and strategies of Caltha palustris were compared for four phytocoeno­ses located in the southern Biebrza basin: sedge reedswamp (Caricetum gracilis, sedge-moss community (Caricetum limoso-diandrae, willow-birch brushwood (Salix cinerea-Betula pubescens, and alder-bog forest (Carici elongatae-Alnetum, The individuals of analysed populations were found to differ with respect to their biomass allocation to generative and vegetative reproduction. Three types of reproductive strategies were distinguished: generative (G, vegetative (V and intermediate (G/V. The individuals growing in the alder-bog forest had the highest reproductive effort (V strategy, while those from sedge reedswamp - the lowest (G strategy.

  11. Lymnaea palustris and Lymnaea fuscus are potential but uncommon intermediate hosts of Fasciola hepatica in Sweden.

    Science.gov (United States)

    Novobilský, Adam; Kašný, Martin; Beran, Luboš; Rondelaud, Daniel; Höglund, Johan

    2013-08-29

    Lymnaea palustris and L. fuscus are members of the European stagnicolines (Gastropoda: Lymnaeidae). The role of stagnicolines in transmission of Fasciola hepatica has been often proposed. To assess the possible relationship between these two stagnicolines and F. hepatica in Sweden, field monitoring in parallel with experimental infections of L. palustris and L. fuscus were conducted. Stagnicoline snails were collected and identified on pastures grazed by either sheep or cattle on four farms suffering from fasciolosis in Sweden during 2011-2012. Field-collected L. palustris and L. fuscus were examined for F. hepatica DNA by PCR. In the laboratory, different age groups of L. palustris, L. fuscus and G. truncatula were each exposed to two F. hepatica miracidia and main infection characteristics were obtained. One field-collected L. palustris (out of n = 668) contained F. hepatica as determined by PCR. On the other hand, stagnicolines artificially exposed to F. hepatica miracidia resulted in successful infection with fully differentiated cercariae, but only in juvenile snails (size, 1-2 mm at exposure) and with a prevalence of 51% and 13% in L. palustris and L. fuscus, respectively. In contrast, 90% of juvenile (size, 1-2 mm) and 92% of preadult G. truncatula (size, ≥ 2-4 mm), respectively, were successfully infected. Delayed, reduced and/or no spontaneous cercarial shedding was observed in the two stagnicolines when compared to G. truncatula. However, at snail dissection most cercariae from L. fuscus and L. palustris were able to encyst similarly to those from G. truncatula. Both L. fuscus and L. palustris can sustain larval development of F. hepatica but with an apparent level of age resistance. The finding of a single F. hepatica positive specimen of L. palustris, together with infection characteristics from the experimental infection, suggest that L. palustris is a more suitable snail vector of F. hepatica than L. fuscus. The reduced growth observed in both

  12. Growth and hydrogen production of Rhodoseudomonas palustris CQK-O1 in a gas sparging photobioreactor%鼓泡式光生物制氢反应器中光合细菌的生长及产氢

    Institute of Scientific and Technical Information of China (English)

    屈晓凡; 廖强; 朱恂; 王永忠; 李俊

    2011-01-01

    Batch culture hydrogen production by the purple non-sulfur phototrophic bacteria Rhodoseudomonas palustris CQK-01 was experimentally investigated in a novel gas sparging photobioreactor. The effects of sparging frequency on the growth and hydrogen production performance of Rhodoseudomonas palustris CQK-01 are discussed. The experimental results showed that sparging Ar gas into the reactor effectively reduced hydrogen concentration in the culture and hence weakened the product feedback inhibition, resulting in enhancement of hydrogen production performance. The gas sparging frequency was found to be an important factor for bacterial growth and hydrogen production by photosynthetic bacteria(PSB), and the optimum hydrogen production performance of the bioreactor was achieved under gas sparging with an interval of 3 h. The total hydrogen production, light conversion efficiency, . Hydrogen yield, average substrate consumption rate and average hydrogen production rate were 33.25 mmol, 18.46%, 1.79 mol (H2)/mol (glucose), 0.28 mmol L"1 IT1 and 0.50 mmol L"1 h~\\ respectively.%提出了一种新型的鼓泡式光生物制氢反应器,并将Rhodopseudomonas palustris CQK-01光合产氢菌在光生物制氢反应器中进行序批式培养,以葡萄糖为碳源底物,以590 nm单波长光为光源,研究了不同鼓泡条件下光合细菌的生长、产氢及底物降解特性.实验结果表明,在光生物制氢反应器中适当鼓入氩气气泡可以有效降低反应器内液相氢浓度,减少产物反馈抑制作用,促进产氢性能的提高,并且不同鼓泡频率对光合细菌的生长和产氢有较大影响.实验中间隔3h鼓泡时光合细菌产氢性能最佳,反应器最大产氢量33.25 mmol,光能转化效率为18.46%,产氢得率为1.79 mol (H2)/mol (glucose),平均底物消耗速率为0.28 mmol L-1 h-1和平均产氢速率为0.50 mmol L-1 h-1.

  13. Biosynthetic and Bioenergetic Functions of Citric Acid Cycle Reactions in Rhodopseudomonas capsulata

    OpenAIRE

    Beatty, J. Thomas; Gest, Howard

    1981-01-01

    Rhodopseudomonas capsulata can grow in a number of alternative modes, including (i) photosynthetic, defined here as anaerobic growth with light as the energy source, and (ii) heterotrophic, referring to aerobic heterotrophic growth in darkness. The functions of citric acid cycle sequences in these growth modes were investigated using wild-type and appropriate mutant strains. Results of growth tests and O2 utilization experiments showed that in the heterotrophic mode, energy conversion is depe...

  14. Nobel lecture. The photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis.

    OpenAIRE

    Deisenhofer, J.; Michel, H

    1989-01-01

    In our lectures we first describe the history and methods of membrane protein crystallization, before we show how the structure of the photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis was solved. Then the structure of this membrane protein complex is correlated with its function as a light-driven electron pump across the photosynthetic membrane. Finally we draw conclusions on the structure of the photosystem II reaction centre from plants and discuss the aspec...

  15. Hybridization of cloned Rhodopseudomonas capsulata photosynthesis genes with DNA from other photosynthetic bacteria.

    OpenAIRE

    Beatty, J T; Cohen, S N

    1983-01-01

    The homology of Rhodopseudomonas capsulata DNA segments carrying photosynthesis genes with sequences present in total DNA from certain other photosynthetic and non-photosynthetic bacterial species was determined by hybridization. R. capsulata DNA fragments that carry loci for production of peptide components of the photosynthetic reaction center and light-harvesting I antenna complex were found to hybridize to DNA from some photosynthetic species. However, fragments that carry carotenoid or b...

  16. Cyanide reduction by nitrogenase in intact cells of Rhodopseudomonas gelatinose Molisch.

    Science.gov (United States)

    Materassi, R; Balloni, W; Florenzano, G

    1977-01-01

    Intact cells of Rhodopseudomonas gelatinosa, grown in N-free medium, reduce cyanide to methane and ammonia at a rate 5--8 times lower than acetylene. Cyanide reduction in whole cells is distinctly more sensitive to cyanide inhibition compared with enzyme preparations. These results are discussed in view of the exploitation of nitrogen-fixing photobacteria in the anaerobic detoxification of cyanide-containing wastewaters.

  17. Biosynthetic and Bioenergetic Functions of Citric Acid Cycle Reactions in Rhodopseudomonas capsulata

    OpenAIRE

    Beatty, J. Thomas; Gest, Howard

    1981-01-01

    Rhodopseudomonas capsulata can grow in a number of alternative modes, including (i) photosynthetic, defined here as anaerobic growth with light as the energy source, and (ii) heterotrophic, referring to aerobic heterotrophic growth in darkness. The functions of citric acid cycle sequences in these growth modes were investigated using wild-type and appropriate mutant strains. Results of growth tests and O2 utilization experiments showed that in the heterotrophic mode, energy conversion is depe...

  18. Innovation durch Paradigmenwechsel - zur Bone Welding® Technologie

    Science.gov (United States)

    Mayer, Jörg; Plasonig, Gerhard

    Innovation entstand und entsteht in der Medizin häufig aus dem Bedürfnis des Klinikers heraus, bestehende chirurgische Techniken zu verbessern oder durch die Einführung neuer Methoden, chirurgische Zugänge zu ermöglichen, welche für den Patienten weniger traumatisch und für den Chirurgen technisch einfacher und damit sicherer sind. Historisch gesehen wurden Innovationen bis weit in die zweite Hälfte des 20. Jahrhunderts vor allem durch Kliniker angeregt und auch massgeblich mitentwickelt. Als illustrative Beispiele hierfür seien die heute immer noch führenden und auch kommerziell sehr erfolgreichen Entwicklungen von Osteosynthesetechniken und Implantaten durch die AO (Arbeitsgemeinschaft für Osteosynthesefragen) oder auch die Einführung endoskopischer Operationstechniken erwähnt.

  19. Draft genome sequence of methanobacterium formicicum DSM 3637, an archaebacterium isolated from the methane producer amoeba pelomyxa palustris 

    OpenAIRE

    Gutiérrez Pozo, Gabriel

    2012-01-01

    Here is reported the draft genome sequence of Methanobacterium formicicum DSM 3637, which was isolated from the methane- producing amoeba Pelomyxa palustris. This bacterium was determined to be an endosymbiont living in the cytoplasm of P. palustris and the source of methane; however, the global characteristics of its genome suggest a free-living lifestyle rather than an endosymbiotic one.

  20. Draft genome sequence of Methanobacterium formicicum DSM 3637, an Archaebacterium isolated from the methane producer amoeba Pelomyxa palustris.

    Science.gov (United States)

    Gutiérrez, Gabriel

    2012-12-01

    Here is reported the draft genome sequence of Methanobacterium formicicum DSM 3637, which was isolated from the methane-producing amoeba Pelomyxa palustris. This bacterium was determined to be an endosymbiont living in the cytoplasm of P. palustris and the source of methane; however, the global characteristics of its genome suggest a free-living lifestyle rather than an endosymbiotic one.

  1. Polar Constituents, Essential Oil and Antioxidant Activity of Marsh Woundwort (Stachys palustris L.).

    Science.gov (United States)

    Venditti, Alessandro; Frezza, Claudio; Bianco, Armandodoriano; Serafini, Mauro; Cianfaglione, Kevin; Nagy, David U; Iannarelli, Romilde; Caprioli, Giovanni; Maggi, Filippo

    2016-12-10

    Stachys palustris, well-known as marsh woundwort, is a perennial herb growing in wet environments of Europe. Its tubers, leaves and seeds are eaten raw or cooked. Alike other Stachys species, the plant is also used as a traditional remedy. Despite S. palustris has been consumed for centuries, little is known about its chemical constituents. In this work the main secondary metabolites of S. palustris from Hungary and France have been analysed. From the plant ethanolic extracts, ethanoid glucosides, isoscutellarein derivatives, caffeoyl-quinic acids and iridoids have been isolated and structurally characterized by NMR. The essential oils were analysed by GC/MS and showed (E)-phytol, fatty acids and carbonylic compounds as the most abundant compounds. The radical scavenging capacity of plant ethanolic extracts, as evaluated by the DPPH assay, was noteworthy, with IC50 values of 92.08 - 105.42 μg/ml.

  2. A theoretical study of electronic excited states of photosynthetic reaction center in Rhodopseudomonas viridis

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The electronic singlet vertical excited states of photosynthetic reaction center (PSRC) in Rhodopseudomonas (Rps.) viridis were investigated by ZINDO and INDO/S methods. The effects of the interactions of pigment-pigment and pigment-protein on the electronic excitations were examined. The calculation results showed that the interactions of pigment-pigment and pigment-protein play an important role in reasonably assigning the experimental absorption and circular dichroism (CD) spectra of PSRC in Rps. virids. By comparing the theoretically computed excited states with the experimental absorption and CD spectra, satisfactory assignments of the experimental spectroscopic peaks were achieved.

  3. Isoprenoid hydrocarbons produced by thermal alteration of Nostoc muscorum and Rhodopseudomonas spheroides

    Science.gov (United States)

    Philp, R. P.; Brown, S.; Calvin, M.

    1978-01-01

    The potential of algae and photosynthetic bacteria to serve as precursors of kerogen was studied to determine what factors affect the relative rates of formation of precursor hydrocarbons. Cells of Nostoc muscorum and Rhodopseudomonas spheroides were subjected to thermal alteration (by heating samples in glass tubes sealed under nitrogen) for two, four, and twelve weeks. Both unextracted and extracted cells in the absence and presence of montmorillonite were investigated, and the isoprenoid hydrocarbons produced in these experiments were determined. Phytane and five isomeric phytenes were the main hydrocarbons observed; their relative rates of formation in the different experimental conditions are described. No phytadienes, pristane, or pristenes were detected.

  4. Gentoxizität durch hormonell stimulierte Proliferation

    OpenAIRE

    Semmel, Britta Birgit

    2006-01-01

    Hormone spielen bei der Kanzerogenese eine wichtige Rolle, indem sie vor allem auf die Phase der Promotion einwirken und die Proliferation bereits initiierter Zellen steigern können. In dieser Arbeit wurden humane Ovarialkarzinomzellen mit Östrogen, Insulin, IGF und EGF zur Proliferation angeregt, woraus eine erhöhte Mikrokernrate resultierte. Mikrokerne sind chromatinhaltige Strukturen, die außerhalb des Zellkerns liegen. Somit lag nahe, dass durch die Steigerung der Proliferation eine genet...

  5. Transformation einer Vorlesung durch E-Learning-Elemente

    Directory of Open Access Journals (Sweden)

    Werner Sesink

    2005-08-01

    Full Text Available Die Veranstaltungsform Vorlesung ist vor allem in geistes- und sozialwissenschaftlichen Studienrichtungen umstritten: mangelnde Aktivierung der Studierenden, kaum Dialog und Diskurs, wenig Möglichkeiten zum Feedback. In diesem Beitrag soll an einem Beispiel aus der Lehrpraxis des Studienfachs Pädagogik an der TU Darmstadt gezeigt werden, dass und wie es möglich ist, eine Vorlesung durch Einsatz von E-Learning-Elementen so zu transformieren, dass das aktive und diskursive Arbeiten mit den Vorlesungsinhalten erfolgreich angeregt wird.

  6. 1-Aminocyclopropane-1-Carboxylate Oxidase Activity Limits Ethylene Biosynthesis in Rumex palustris during Submergence

    Science.gov (United States)

    Vriezen, Wim H.; Hulzink, Raymond; Mariani, Celestina; Voesenek, Laurentius A.C.J.

    1999-01-01

    Submergence strongly stimulates petiole elongation in Rumex palustris, and ethylene accumulation initiates and maintains this response in submerged tissues. cDNAs from R. palustris corresponding to a 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene (RP-ACO1) were isolated from elongating petioles and used to study the expression of the corresponding gene. An increase in RP-ACO1 messenger was observed in the petioles and lamina of elongating leaves 2 h after the start of submergence. ACC oxidase enzyme activity was measured in homogenates of R. palustris shoots, and a relevant increase was observed within 12 h under water with a maximum after 24 h. We have shown previously that the ethylene production rate of submerged shoots does not increase significantly during the first 24 h of submergence (L.A.C.J. Voesenek, M. Banga, R.H. Thier, C.M. Mudde, F.M. Harren, G.W.M. Barendse, C.W.P.M. Blom [1993] Plant Physiol 103: 783–791), suggesting that under these conditions ACC oxidase activity is inhibited in vivo. We found evidence that this inhibition is caused by a reduction of oxygen levels. We hypothesize that an increased ACC oxidase enzyme concentration counterbalances the reduced enzyme activity caused by low oxygen concentration during submergence, thus sustaining ethylene production under these conditions. Therefore, ethylene biosynthesis seems to be limited at the level of ACC oxidase activity rather than by ACC synthase in R. palustris during submergence. PMID:10482674

  7. Identification of floral genes for sex determination in Calamus palustris Griff. by using suppression subtractive hybridization.

    Science.gov (United States)

    Ng, C Y; Wickneswari, R; Choong, C Y

    2014-08-07

    Calamus palustris Griff. is an economically important dioecious rattan species in Southeast Asia. However, dioecy and onset of flowering at 3-4 years old render uncertainties in desired female:male seedling ratios to establish a productive seed orchard for this rattan species. We constructed a subtractive library for male floral tissue to understand the genetic mechanism for gender determination in C. palustris. The subtractive library produced 1536 clones with 1419 clones of high quality. Reverse Northern screening showed 313 clones with differential expression, and sequence analyses clustered them into 205 unigenes, including 32 contigs and 173 singletons. The subtractive library was further validated with reverse transcription-quantitative polymerase chain reaction analysis. Homology identification classified the unigenes into 12 putative functional proteins with 83% unigenes showing significant match to proteins in databases. Functional annotations of these unigenes revealed genes involved in male flower development, including MADS-box genes, pollen-related genes, phytohormones for flower development, and male flower organ development. Our results showed that the male floral genes may play a vital role in sex determination in C. palustris. The identified genes can be exploited to understand the molecular basis of sex determination in C. palustris.

  8. Morphological variability of the Caltha palustris L. complex (Ranunculaceae in Poland

    Directory of Open Access Journals (Sweden)

    Elżbieta Cieślak

    2011-01-01

    Full Text Available The paper presents results of biosystematic analysis of the critical group of Caltha palustris L. based on 71 populations from Poland. After a preliminary statistical analysis, fourteen morphological characters (nine quantitative and five qualitative, describing size and shape of basal leaves and mature follicles as well as stem morphology, were selected for the clearest differentiation of the complex. Several groups of morphotypes were distinguished within the complex, based on the statistical analysis of this group of characters. Against this background the taxonomy of the complex is proposed. Two species - Caltha laeta Schott, Nyman and Kotschy and Caltha palustris L. - and three subspecies within C. palustris (C. p. subsp. palustris, C. p. subsp. cornuta (Schott, Nyman and Kotschy Hegi and C. p. subsp. radicans (T.F. Forst. Syme, were distinguished in Poland. The status of the taxa within the Polish flora is defined. The paper includes also a key for determination of the complex taxa, as well as their detailed descriptions.

  9. Untersuchungen zur Trockenentschwefelung von Brenngasen durch Partialoxidation von H2S an Herdofenkoks

    OpenAIRE

    Bauersfeld, Dirk

    2009-01-01

    Die vorliegende Arbeit befasst sich mit Untersuchungen zur Trockenentschwefelung von Brenngasen durch Partialoxidation von H2S an Herdofenkoks. Hierzu wurden Versuche in der Technikumsanlage VTE 2004 mit einem simulierten PHTW Gas durchgeführt. Es zeigte sich, dass der COS-Abbau nicht wie bisher angenommen durch die COS-Partialoxidation sondern durch die COS-Hydrolyse erfolgt. Die COS-Hydrolyse gewinnt dabei mit abnehmender Raumbelastung an Bedeutung. Der Entschwefelungsgrad erhöht sich mit s...

  10. Cholinesterase inhibitory activity and chemical constituents of Stenochlaena palustris fronds at two different stages of maturity

    Directory of Open Access Journals (Sweden)

    Nelson Jeng-Yeou Chear

    2016-04-01

    Full Text Available Stenochlaena palustris fronds are popular as a vegetable in Southeast Asia. The objectives of this study were to evaluate the anticholinesterase properties and phytochemical profiles of the young and mature fronds of this plant. Both types of fronds were found to have selective inhibitory effect against butyrylcholinesterase compared with acetylcholinesterase. However, different sets of compounds were responsible for their activity. In young fronds, an antibutyrylcholinesterase effect was observed in the hexane extract, which was comprised of a variety of aliphatic hydrocarbons, fatty acids, and phytosterols. In the mature fronds, inhibitory activity was observed in the methanol extract, which contained a series of kaempferol glycosides. Our results provided novel information concerning the ability of S. palustris to inhibit cholinesterase and its phytochemical profile. Further research to investigate the potential use of this plant against Alzheimer's disease is warranted, however, young and mature fronds should be distinguished due to their phytochemical differences.

  11. Vergesellschaftung und Ökologie der Sumpf-Siegwurz (Gladiolus palustris) in Südbayern

    OpenAIRE

    Schmitt, Barbara; Fartmann, Thomas; Hölzel, Norbert

    2014-01-01

    Die seltene und in den Anhängen II und IV der FFH-Richtlinie aufgeführte Sumpf-Siegwurz (Gladiolus palustris) kommt deutschlandweit in größeren Populationen nur noch in Südbayern vor. Trotz der hohen naturschutzfachlichen Bedeutung liegen keine überregionalen wissenschaftlichen Studien zur Vergesellschaftung und Ökologie der Art vor. Diese Arbeit analysiert mit Hilfe von numerischen Klassifikations- und Ordinationsverfahren anhand von 151 Vegetationsaufnahmen die Vergesellschaftung und Stando...

  12. Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale.

    Science.gov (United States)

    Le Roux, Christine; Tournier, Estelle; Lies, Adrien; Sanguin, Hervé; Chevalier, Gérard; Duponnois, Robin; Mousain, Daniel; Prin, Yves

    2016-01-01

    This work aimed at characterizing 12 isolates of the genus Tuber including Tuber melanosporum (11 isolates) and Tuber brumale (one isolate). This was done using internal transcribed spacer (ITS) sequences, confirming their origin. Analysis of their mating type revealed that both MAT1-1 and MAT1-2 exist within these isolates (with 3 and 8 of each, respectively). We observed that each of these cultures was consistently associated with one bacterium that was intimately linked to fungal growth. These bacterial associates failed to grow in the absence of fungus. We extracted DNA from bacterial colonies in the margin of mycelium and sequenced a nearly complete 16S rDNA gene and a partial ITS fragment. We found they all belonged to the genus Rhodopseudomonas, fitting within different phylogenetic clusters. No relationships were evidenced between bacterial and fungal strains or mating types. Rhodopseudomonas being a sister genus to Bradyrhizobium, we tested the nodulation ability of these bacteria on a promiscuously nodulating legume (Acacia mangium), without success. We failed to identify any nifH genes among these isolates, using two different sets of primers. While the mechanisms of interaction between Tuber and Rhodopseudomonas remain to be elucidated, their interdependency for in vitro growth seems a novel feature of this fungus.

  13. Amerikanisierung durch Internationalisierung: Die Expansion der International Communication Association (ICA

    Directory of Open Access Journals (Sweden)

    Thomas Wiedemann

    2016-12-01

    Full Text Available Basierend auf der Soziologie Bourdieus problematisiert dieser Beitrag die Bemühungen der International Communication Association (ICA, sich über die Öffnung ihrer Führungsetage für Wissenschaftler außerhalb der USA in eine wahrhaft internationale Fachgesellschaft zu verwandeln und der Herausforderung einer global vernetzten Disziplin zu begegnen. Geleistet werden soll so ein kritischer Beitrag zur Selbstreflexion der Kommunikationswissenschaft, zu verstehen als Deutungsangebot und Ausgangspunkt für die wissenschaftliche Diskussion. Die Untersuchung von Habitus und Kapital der 29 ICA-Präsidenten und ICA Fellows aus der internationalen Scientific Community zeigt, dass die weltweit größte kommunikationswissenschaftliche Fachgesellschaft trotz der Ausweitung ihrer Führungsriege immer noch deutlichen US-Einflüssen unterliegt. Die neuen ICAWürdenträger, die für nationale und fachliche Vielfalt stehen sollen, stammen aus Weltregionen, die eine besondere Nähe zu den Vereinigten Staaten auszeichnet, und wurden an US-Universitäten sozialisiert oder stark von der US-amerikanischen Forschungstradition geprägt. Ausnahmen („Einkäufe“ von führenden Vertretern anderer Fachgesellschaften oder alternativer Paradigmen bestätigen die Regel. Die Internationalisierung der ICA-Führungsetage veränderte demzufolge weniger den Machtpol im Fach als vielmehr die Kommunikationswissenschaft weltweit. Zwar gelangten neue Perspektiven ins Zentrum der Disziplin. Im Gegenzug fand jedoch eine Amerikanisierung nationaler Felder statt, allen voran durch ICA Fellows als Vorbilder im Kampf um wissenschaftliches Kapital. Die Bemühungen der ICA, sich durch die Expansion ihrer Führungsriege zu internationalisieren, dürften somit die Machtstrukturen im Fach weiter verfestigt haben.

  14. Endemic Marsh Mongoose Herpestes palustris (Carnivora: Herpestidae of East Kolkata Wetlands, India: a status report

    Directory of Open Access Journals (Sweden)

    J.K. Mallick

    2009-04-01

    Full Text Available Marsh Mongoose Herpestes palustris is the only extant endemic mammal of the East Kolkata wetlands, which has been declared a RAMSAR site in 2002. Since its first description by the scientists of the Zoological Survey of India, the population of this species has dwindled to an alarming state due to reclamation of the Salt Lake City and Rajarhat expansion, as well as from other anthropogenic causes. Recently, during a field survey only a small population of this endangered mongoose was found in a single location. Immediate conservation measures are required to be taken by the concerned authorities to stop its probable extinction in the near future.

  15. Energy transfer via sunshades and light deflection elements; Energietransport durch Sonnenschutzsysteme und Lichtlenkelemente

    Energy Technology Data Exchange (ETDEWEB)

    Haug, I.

    2001-07-01

    The author investigated energy transfer via sunshades and light deflection elements. The information will be required for the development of external wall elements. The investigations are funded by Deutsche Bundesstiftung Umwelt. [German] Im Rahmen dieser Arbeit wurde der Energietransport durch eine herkoemmliche Waermeschutzverglasung mit innen liegender Jalousie, durch eine neuartige, lichtumlenkende LIF-Verglasung und durch eine lichtstreuende Kapillarverglasung untersucht. - Die Kenntnisse ueber den Energietransport werden benoetigt, um energetische Kennzahlen fuer Fassadenelemente bestimmen zu koennen, die in der Bauphysik und hinsichtlich des aktuellen Gesetzgebungsverfahren eine wesentliche Rolle spielen. - Der Energietransport, der sich aus Strahlungstransport und Waermetransport zusammensetzt, und die zugehoerigen energetischen Kennzahlen wurden fuer die genannten Systeme modelliert und messtechnisch erfasst. Diese Arbeit wurde durch die Deutsche Bundesstiftung Umwelt gefoerdert. (orig.)

  16. Israelische Public Diplomacy und ihre Wahrnehmung durch deutsche Journalisten

    Directory of Open Access Journals (Sweden)

    Florian Fleischer

    2014-07-01

    Full Text Available Public Diplomacy stellt insbesondere für Staaten in internationalen Konflikten wie Israel ein wichtiges Kommunikationsinstrument dar, um ausländische Bevölkerungen zu erreichen. Der Artikel zeigt die Ergebnisse einer Studie, in der untersucht wurde, wie die israelische Public Diplomacy von deutschen Journalisten aufgenommen und verarbeitet wird. Journalisten kommt dabei eine Schlüsselrolle als Mittler zwischen dem israelischen Staat und der deutschen Bevölkerung zu. In Anlehnung an den Katalog der Public Diplomacy-Maßnahmen von Gilboa (2006 wurde die Relevanz einzelner Maßnahmen für Journalisten erfragt. Die Ergebnisse zeigen, dass persönliche Kontakte für Journalisten nach wie vor eine wichtige Quelle darstellen und nicht von Cyber-Public Diplomacy ersetzt werden können. Staatliche Public Diplomacy wird zwar wahrgenommen, jedoch wird ihre Objektivität bezweifelt, während NGOs und Stiftungen als vertrauenswürdiger eingestuft werden. Des Weiteren wird Israel in der Öffentlichkeit nach wie vor dominierend mit dem Nahostkonflikt identifiziert, was sich nach Meinung der befragten Journalisten auch in Zukunft nicht durch gezielte Öffentlichkeitsarbeit verhindern lässt.

  17. Beeinflussung der Schilddrüsenfunktion durch Medikamente

    Directory of Open Access Journals (Sweden)

    Wolf P

    2014-01-01

    Full Text Available Die Funktion der Schilddrüse unterliegt einem strengen Regelkreis, der zahlreiche Angriffspunkte für unerwünschte Wechselwirkungen mit verschiedenen Medikamenten und Wirkstoffklassen bietet, teilweise mit klinisch relevanten Folgen. In dieser Übersichtsarbeit sollen daher einerseits die häufigsten Medikamentenwirkungen auf die Schilddrüsenfunktion beschrieben sowie andererseits auch auf die Folgen einer gestörten Resorption bei bereits bestehender Schilddrüsenhormonsubstitutionstherapie hingewiesen werden. Während diese Wechselwirkungen beispielsweise für Amiodaron oder Lithium schon lange bekannt sind, kommt es vor allem durch die Entwicklung neuer immunmodulierender Wirkstoffklassen, wie Tyrosinkinaseinhibitoren und monoklonaler Autoantikörper, zu gehäuften Beobachtungen von Schilddrüsenfunktionsbeeinträchtigungen. Neben Lithium wird auch auf die Wechselwirkung anderer Psychopharmaka eingegangen sowie der Einfluss von Wirkstoffklassen wie Gallensäurebinder, Antazida und Phosphatbinder auf die Resorption von Levothyroxin bei bestehender Schilddrüsenhormonsubstitutionstherapie näher beschrieben. Das Ziel dieser Arbeit ist es daher, einen für den praktisch tätigen Arzt tauglichen Überblick über Medikamentenwirkungen auf die Schilddrüse zu schaffen.

  18. Keratitis with Kocuria palustris and Rothia mucilaginosa in Vitamin A Deficiency

    Directory of Open Access Journals (Sweden)

    R.M. Mattern

    2014-02-01

    Full Text Available Purpose: To present a case of unusual corneal infection early in the course of peripheral ulcerative keratitis in a patient with severe vitamin A deficiency. Method: Single observational case report in urban USA. Case Presentation: An alcoholic patient with pancreatitis, chronic diarrhea, and vitamin A deficiency presented with a marginal corneal ulcer from which two bacteria of the family Micrococcaceae were cultured and identified by genome sequence analysis, namely Kocuria palustris and Rothia mucilaginosa. Soon after, severe bilateral peripheral ulcerative keratitis developed, later accompanied by eyelid cellulitis of one lid. These conditions improved with antibiotics, treatment of the underlying gastrointestinal conditions, and treatment of the vitamin deficiency. Conclusion: Susceptibility to keratitis with unusual bacteria of the Micrococcaceae family can occur in the setting of alcoholism-related gastrointestinal disease with severe vitamin A deficiency. To our knowledge, K. palustris is a species not previously identified in any human disease, and the Kocuria genus has not previously been reported as a participant in eye infection. Documented cases of R. mucilaginosa in ocular disease are rare. These unusual infections heralded the onset of severe marginal corneal melts.

  19. Random walk, zonation and the food searching strategy of Terebralia palustris (Mollusca, Potamididae) in Kenya

    Science.gov (United States)

    Vannini, Marco; Cannicci, Stefano; Mrabu, Elisha; Rorandelli, Rocco; Fratini, Sara

    2008-12-01

    Terebralia palustris is a common mud-whelk present at a particularly high density in all Indo-West Pacific mangroves. Young snails feed on nothing but mud while larger specimens are able to feed on fallen leaves too. In Kenya (Mida Creek) under the canopy, competition for mangrove leaves can be very high due to the high density of Sesarmidae crabs. On open exposed muddy platforms, no Sesarmidae occur but the leaf density is very low because the leaves are only randomly present as they are deposited and removed twice a day by the tide. However, the snail density is always very high, raising the question as to whether the snails use a special searching strategy to optimize their resource finding rather than a purely random movement. By analyzing the snails' movements on a uniform area at different levels and comparing them with simulated random paths, we could show that the snails' movements are not purely random. The distribution of different size classes of T. palustris in Mida Creek was known to be quite odd: the same simulation approach suggests that the zonation asymmetry could reasonably be due to the stochastic recruitment of juveniles in space and time and maintained by a substantial long-lasting spatial inertia.

  20. The potential of Thelypteris palustris and Asparagus sprengeri in phytoremediation of arsenic contamination.

    Science.gov (United States)

    Anderson, LaShunda L; Walsh, Maud; Roy, Amitava; Bianchetti, Christopher M; Merchan, Gregory

    2011-02-01

    The potential of two plants, Thelypteris palustris (marsh fern) and Asparagus sprengeri (asparagus fern), for phytoremediation of arsenic contamination was evaluated. The plants were chosen for this study because of the discovery of the arsenic hyperaccumulating fern, Pteris vittata (Ma et al., 2001) and previous research indicating asparagus fern's ability to tolerate > 1200 ppm soil arsenic. Objectives were (1) to assess if selected plants are arsenic hyperaccumulators; and (2) to assess changes in the species of arsenic upon accumulation in selected plants. Greenhouse hydroponic experiments arsenic treatment levels were established by adding potassium arsenate to solution. All plants were placed into the hydroponic experiments while still potted in their growth media. Marsh fern and Asparagus fern can both accumulate arsenic. Marsh fern bioaccumulation factors (> 10) are in the range of known hyperaccumulator, Pteris vittata Therefore, Thelypteris palustris is may be a good candidate for remediation of arsenic soil contamination levels of arsenic. Total oxidation of As (III) to As (V) does not occur in asparagus fern. The asparagus fern is arsenic tolerant (bioaccumulation factors < 10), but is not considered a good potential phytoremediation candidate.

  1. Moerasmelkdistel (Sonchus palustris L.) en Grote engelwortel (Angelica archangelica L.) langs het Twentekanaal: indicatoren van gebiedsvreemd water

    NARCIS (Netherlands)

    Boedeltje, G.

    1991-01-01

    In the Netherlands Sonchus palustris is a species characteristic of the borders of mostly oligohaline waters. Its optimum is in the western part of the country. In 1934 Angelica archangelica was found for the first time in the Netherlands along the river Waal. Since that time this species has expand

  2. Ethylene regulates fast apoplastic acidification and expansin A transcription during submergence-induced petiole elongation in Rumex palustris

    NARCIS (Netherlands)

    Vreeburg, RAM; Benschop, JJ; Peeters, AJM; Colmer, TD; Ammerlaan, AHM; Staal, M; Elzenga, TM; Staals, RHJ; Darley, CP; McQueen-Mason, SJ; Voesenek, LACJ

    2005-01-01

    The semi-aquatic dicot Rumex palustris responds to complete submergence by enhanced elongation of young petioles. This elongation of petiole cells brings leaf blades above the water surface, thus reinstating gas exchange with the atmosphere and increasing survival in flood-prone environments. We alr

  3. Exciton Dynamics in LH1 and LH2 of Rhodopseudomonas Acidophila and Rhodobium Marinum Probed with Accumulated Photon Echo and Pump-Probe Measurements

    NARCIS (Netherlands)

    Lampoura, Stefania S.; Grondelle, Rienk van; Stokkum, Ivo H.M. van; Cogdell, Richard J.; Wiersma, Douwe A.; Duppen, Koos; van Stokkum, I.H.N.

    2000-01-01

    Exciton dynamics in the B850 and B875 bands of isolated complexes of Rhodopseudomonas acidophila (strain 10050 and 7050) and in the B875 band of isolated complexes of Rhodobium marinum were investigated by means of accumulated photon echo and pump-probe techniques at different temperatures and wavel

  4. The phosphoenolpyruvate-dependent fructose-specific phosphotransferase system in Rhodopseudomonas sphaeroides : Energetics of the phosphoryl group transfer from phosphoenolpyruvate to fructose

    NARCIS (Netherlands)

    Lolkema, Juke S.; Hoeve-Duurkens, Ria H. ten; Robillard, George T.

    1986-01-01

    Energy coupling to fructose transport in Rhodopseudomonas sphaeroides is achieved by phosphorylation of the membrane-spanning fructose-specific carrier protein, EIIFru. The phosphoryl group of phosphoenolpyruvate is transferred to EIIFru via the cytoplasmic component SF (soluble factor). The standar

  5. The phosphoenolpyruvate-dependent fructose-specific phosphotransferase system in Rhodopseudomonas sphaeroides : Distribution of EIIFru over the membranes of phototrophically grown Rps. sphaeroides

    NARCIS (Netherlands)

    Lolkema, Juke S.; Hoeve-Duurkens, Ria H. ten; Robillard, George T.

    1986-01-01

    The distribution of the fructose carrier over the membranes of Rhodopseudomonas sphaeroides was studied in cells grown under light saturation and light limitation. Three types of membranes were isolated after disruption of the cells in a French press. All three types were present in the cells grown

  6. The Transmembrane Electrical Potential in Rhodopseudomonas sphaeroides Determined from the Distribution of Tetraphenylphosphonium after Correction for Its Binding to Cell Components

    NARCIS (Netherlands)

    Lolkema, Juke S.; Abbing, Arend; Hellingwerf, K; Konings, Wilhelmus

    1983-01-01

    The membrane potential was determined in intact cells of Rhodopseudomonas sphaeroides from the distribution of the lipophilic cation tetraphenylphosphonium (Ph4P+) after correction for probe binding to cell components. The concentration of Ph4P+ in the external medium of the cells was recorded with

  7. EFEK IMUNOMODULATOR EKSTRAK AIR CINCAU HITAM (Mesona palustris BL TERHADAP KARSINOGENESIS MENCIT [Immunomodulatory Effects of Water Extracts of Black Cincau (Mesona palustris BL on Carcinogenesis in Mice

    Directory of Open Access Journals (Sweden)

    Tri Dewanti W.1*

    2012-06-01

    Full Text Available Janggelan or black cincau (Mesona palustris BL is commonly consumed as dessert and also used as herbal remedy in the folk medicine. This study aimed to test the potency of water extract of black cincau in inhibiting fibrosarcoma cancer induced by benzo(apyrene. Mice used in this study were divided into 6 groups. Solution of benzo(apyrene 0.3% was given during two days for five times to induce cancer. Water extract of black cincau at concentration of 100, 500 and 1000 mg/kg BW was given daily for 2 weeks before initiation (induction of benzo(apyrene during the initiation and until 4 weeks after initiation. The IFN-γ was observed with IFN-y ELISA Kit immunosurveillance parameters while the CD8+, NK cells and macrophages were observed with a flow cytometer. Cancer incidence was observed by palpation every week until 10 weeks after benzo(apyrene last induction. The results showed that a high dose of water extract of black cincau (1000 mg/kg BW increased IFN-γ expression and immunosurveillance of CD8+, NK cells and macrophages of the mice. The treatment also increased cell apoptosis and reduced the cancer incidence in mice by 57%.

  8. Experiments on accumulation of phosphorus in the plants Myosotis palustris, Glyceria maxima and Nasturtium officinale

    Directory of Open Access Journals (Sweden)

    O. Prokopchuk

    2016-10-01

    Full Text Available The problem of availability of quality water is highly relevant today, so the technologies of prediction and prevention of water pollution and purification are very important. Biological methods of cleaning, in paticular cleaning water by the so-called method of biosorption, have been increasingly used in the last decade. This method means the removal of dangerous substances and improvement of water condition by using aquatic organisms, in particular plants. Therefore, in view of the rich experience of research conducted in the biosorption sphere, we decided to predict the effectiveness of this method by using the cumulative ability of higher water plants to absorb phosphorus compounds. For this purpose, we selected water and plant samples (Glyceria maxima (C. Hartm. Holmb., Nasturtium officinale R. Br., Myosotis palustris (L. L. from the river Seret (Ternopil, Ukraine. The plants were placed into sterilized glass jars filled with 3 liters of water from the river Seret (control samples and still tap water with addition of sodium phosphate with phosphorus concentration of 3.5 mg/dm³ (research sample, which were cultured in laboratory conditions for four months. We determined the content of phosphates, permanganate and dichromate oxidation in the water and the total content of phosphorus in the plants. We traced the dynamic of organic substances and the content of phosphates in the water, the accumulation of phosphorus in plants and the rate of accumulation of phosphorus in the plants and in the water. We calculated correlation coefficients to detect the dependence between phosphorus indicators in the aquatic plants and the concentration of phosphate ions in the water. We found that M. palustris had the greatest capacity to accumulate phosphorus and the highest rate of phosphorus accumulation from water, which allows us to consider it the most effective aquatic plant for absorption of elements and decreasing water pollution. We also established

  9. Population structure and mating-type genes of Colletotrichum graminicola from Agrostis palustris.

    Science.gov (United States)

    Chen, Fajun; Goodwin, Paul H; Khan, Adalat; Hsiang, Tom

    2002-05-01

    Eighty-seven isolates of Colletotrichum graminicola, mostly from Agrostis palustris, were collected in grass fields, most of which were in Ontario, Canada. Specific primers were designed to amplify the mating-type (MAT) genes and, among 35 isolates tested, all yielded a band of the expected size for MAT2. For six isolates, the MAT2 PCR products were sequenced and found to be similar to that reported for MAT2 of C. graminicola from maize. Based on 119 polymorphic bands from 10 random amplified polymorphic DNA primers, analyses of genetic distances were found to generally cluster isolates by host and geographic origin. Among 42 isolates from a grass field in Ontario, significant spatial autocorrelation was found to occur within a 20-m distance, implying that this is the effective propagule dispersal distance. Although clonal propagation was observed in the 87 isolates with 67 unique genotypes, the extent of genetic variation in local populations implies some occurrence of sexual or asexual recombination.

  10. Current status of Marsh Crocodiles Crocodylus palustris (Reptilia: Crocodylidae in Vishwamitri River, Vadodara City, Gujarat, India

    Directory of Open Access Journals (Sweden)

    R. Vyas

    2012-11-01

    Full Text Available Data presented here is based on a three year study (2008-2010 on a population of Mugger Crocodylus palustris inhabiting Vishwamitri River near Vadodara City, Gujarat State, India. In total, 155 Muggers were counted in the 25km river stretch during 2010. In all, 40 burrows were observed along the river bank, and the same were clumped in certain sections of the river. Muggers fed eight species of birds, and domestic livestock in addition to scavenging. Eight instances of human-crocodile conflicts were observed including four human causalities. A total 90 Muggers were rescued from the urban areas and the same were relocated elsewhere in the river system. Various types of threats to Mugger were also noticed including habitat loss, alteration and soil erosion and mortality due to rail traffic. The present study suggests further research to propose strategies to conserve this population.

  11. Effects of Restoration Techniques on Soil Carbon and Nitrogen Dynamics in Florida Longleaf Pine (Pinus palustris) Sandhill Forests

    OpenAIRE

    Martin Lavoie; Mack, Michelle C; John K. Hiers; Scott Pokswinski; Analie Barnett; Louis Provencher

    2014-01-01

    Historic fire suppression and intensive forest management in longleaf pine (Pinus palustris) sandhill forests has resulted in hardwood encroachment and degradation of this fire-dependent ecosystem. Active management is now required to restore native community structure and composition, but little is known about the long-term impacts of typical restoration techniques on ecosystem properties. In 1994, the Longleaf Pine Restoration Project (LPRP) was established in fire-excluded longleaf pine sa...

  12. Bioremediation of CCA-Treated Wood By Brown-Rot Fungi Fomitopsis Palustris, Coniophora Puteana, and Laetiporus Sulphureus

    OpenAIRE

    Kartal, S Nami; Munir, Erman; Kamitani, Tomo

    2008-01-01

    This study evaluated oxalic acid accumulation and bioremediation of chromated copper arscnate (CCA)-treated wood by three brown-rot fungi Fomitopsis palustris Coniophora puteane, and Laetiporus sulphureas. The fungi were first cultivated in a fermentation broth to accumulate oxalic acid. Bioremediation of CCA-treated wood was then carried out by leaching of heavy metals with oxalic acid over a 10-day fermentation period. Higher amounts of oxalic acid were produced by F. polustris and L. sulp...

  13. Evidence against observational spatial memory for cache locations of conspecifics in marsh tits Poecile palustris.

    Science.gov (United States)

    Urhan, A Utku; Emilsson, Ellen; Brodin, Anders

    2017-01-01

    Many species in the family Paridae, such as marsh tits Poecile palustris, are large-scale scatter hoarders of food that make cryptic caches and disperse these in large year-round territories. The perhaps most well-known species in the family, the great tit Parus major, does not store food itself but is skilled in stealing caches from the other species. We have previously demonstrated that great tits are able to memorise positions of caches they have observed marsh tits make and later return and steal the food. As great tits are explorative in nature and unusually good learners, it is possible that such "memorisation of caches from a distance" is a unique ability of theirs. The other possibility is that this ability is general in the parid family. Here, we tested marsh tits in the same experimental set-up as where we previously have tested great tits. We allowed caged marsh tits to observe a caching conspecific in a specially designed indoor arena. After a retention interval of 1 or 24 h, we allowed the observer to enter the arena and search for the caches. The marsh tits showed no evidence of such observational memorization ability, and we believe that such ability is more useful for a non-hoarding species. Why should a marsh tit that memorises hundreds of their own caches in the field bother with the difficult task of memorising other individuals' caches? We argue that the close-up memorisation procedure that marsh tits use at their own caches may be a different type of observational learning than memorisation of caches made by others. For example, the latter must be done from a distance and hence may require the ability to adopt an allocentric perspective, i.e. the ability to visualise the cache from the hoarder's perspective. Members of the Paridae family are known to possess foraging techniques that are cognitively advanced. Previously, we have demonstrated that a non-hoarding parid species, the great tit P. major, is able to memorise positions of caches that

  14. Toxicity of sulfide to early life-stages of wild rice (Zizania palustris).

    Science.gov (United States)

    Fort, Douglas J; Todhunter, Kevin; Fort, Troy D; Mathis, Michael B; Walker, Rachel; Hansel, Mike; Hall, Scott; Richards, Robin; Anderson, Kurt

    2017-02-07

    Wild rice (Zizania palustris) sensitivity to sulfide is not well understood. Since sulfate in surface waters is reduced to sulfide by anaerobic bacteria in sediments and historical information indicated that 10 mg/L sulfate in Minnesota surface water reduced Z. palustris abundance, the Minnesota Pollution Control Agency (MPCA) established 10 mg/L sulfate as a water quality criterion in 1973. A 21-day daily-renewal hydroponic study was conducted to evaluate sulfide toxicity to wild rice and the potential mitigation of sulfide toxicity by Fe. The hydroponic design used hypoxic test media for seed and root exposure and aerobic headspace for the vegetative portion of the plant. Test concentrations were 0.3, 1.6, 3.1, 7.8, and 12.5 mg/L sulfide in test media with 0.8, 2.8, and 10.8 mg/L total Fe used to evaluate the impact of iron on sulfide toxicity. Visual assessments (i.e., no plants harvested) of seed activation, mesocotyl emergence, seedling survival, and phytoxicity were conducted 10 days following dark-phase exposure. Each treatment was also evaluated for time to 30% emergence (ET30), total plant biomass, root and shoot lengths, and signs of phytotoxicity at study conclusion (21 days). Results from this study indicated that exposure of developing wild rice to sulfide at ≥3.1 mg sulfide/L in the presence of 0.8 mg/L iron reduced mesocotyl emergence. Sulfide toxicity was mitigated by the addition of iron at 2.8 and 10.8 mg /L relative to the control value of 0.8 mg Fe/L, demonstrating the importance of iron in mitigating sulfide toxicity to wild rice. Ultimately, determination of site-specific sulfate criteria that consider factors that alter toxicity, including sediment Fe and organic carbon, are necessary. This article is protected by copyright. All rights reserved.

  15. NCBI nr-aa BLAST: CBRC-XTRO-01-2525 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-2525 ref|NP_947919.1| putative alginate o-acetyltransferase AlgI [Rhod...opseudomonas palustris CGA009] emb|CAE28018.1| putative alginate o-acetyltransferase AlgI [Rhodopseudomonas palustris CGA009] NP_947919.1 0.79 37% ...

  16. NCBI nr-aa BLAST: CBRC-AGAM-05-0012 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-05-0012 ref|YP_781556.1| filamentous haemagglutinin family outer membrane... protein [Rhodopseudomonas palustris BisA53] gb|ABJ06576.1| filamentous haemagglutinin family outer membrane protein [Rhodopseudomonas palustris BisA53] YP_781556.1 0.001 26% ...

  17. NCBI nr-aa BLAST: CBRC-HSAP-02-0064 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-02-0064 ref|YP_534057.1| hypothetical protein RPC_4214 [Rhodopseudomonas ...palustris BisB18] gb|ABD89738.1| hypothetical protein RPC_4214 [Rhodopseudomonas palustris BisB18] YP_534057.1 6.2 24% ...

  18. NCBI nr-aa BLAST: CBRC-CJAC-01-1274 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-1274 ref|NP_945566.1| possible OmpA family member [Rhodopseudomonas pa...lustris CGA009] emb|CAE25657.1| possible OmpA family member [Rhodopseudomonas palustris CGA009] NP_945566.1 1e-09 28% ...

  19. NCBI nr-aa BLAST: CBRC-ACAR-01-1002 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-1002 ref|YP_534416.1| NADH dehydrogenase (quinone) [Rhodopseudomonas p...alustris BisB18] gb|ABD90097.1| NADH dehydrogenase (quinone) [Rhodopseudomonas palustris BisB18] YP_534416.1 0.56 28% ...

  20. NCBI nr-aa BLAST: CBRC-LAFR-01-0681 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-0681 ref|YP_783685.1| periplasmic sensor hybrid histidine kinase [Rhodopseudomonas... palustris BisA53] gb|ABJ08705.1| periplasmic sensor hybrid histidine kinase [Rhodopseudomonas palustris BisA53] YP_783685.1 3.5 33% ...

  1. NCBI nr-aa BLAST: CBRC-SARA-01-0844 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-0844 ref|NP_949451.1| putative conjugal transfer protein trbI [Rhodopseudomonas... palustris CGA009] emb|CAE29556.1| putative conjugal transfer protein trbI [Rhodopseudomonas palustris CGA009] NP_949451.1 0.38 29% ...

  2. NCBI nr-aa BLAST: CBRC-TSYR-01-1170 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TSYR-01-1170 ref|YP_001989230.1| protein of unknown function DUF214 [Rhodopseudomonas... palustris TIE-1] gb|ACE98754.1| protein of unknown function DUF214 [Rhodopseudomonas palustris TIE-1] YP_001989230.1 0.021 27% ...

  3. NCBI nr-aa BLAST: CBRC-BTAU-01-2653 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-BTAU-01-2653 ref|YP_780396.1| glycosyl transferase, group 1 [Rhodopseudomonas ...palustris BisA53] gb|ABJ05416.1| glycosyl transferase, group 1 [Rhodopseudomonas palustris BisA53] YP_780396.1 5.7 23% ...

  4. NCBI nr-aa BLAST: CBRC-MEUG-01-2127 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MEUG-01-2127 ref|YP_781289.1| acyltransferase 3 [Rhodopseudomonas palustris Bi...sA53] gb|ABJ06309.1| acyltransferase 3 [Rhodopseudomonas palustris BisA53] YP_781289.1 8.4 40% ...

  5. NCBI nr-aa BLAST: CBRC-PABE-26-0217 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-26-0217 ref|YP_568915.1| inner-membrane translocator [Rhodopseudomonas pa...lustris BisB5] gb|ABE39014.1| inner-membrane translocator [Rhodopseudomonas palustris BisB5] YP_568915.1 0.88 25% ...

  6. NCBI nr-aa BLAST: CBRC-XTRO-01-1921 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-1921 ref|YP_778945.1| hypothetical protein RPE_0004 [Rhodopseudomonas ...palustris BisA53] gb|ABJ03965.1| conserved hypothetical protein [Rhodopseudomonas palustris BisA53] YP_778945.1 1e-06 30% ...

  7. NCBI nr-aa BLAST: CBRC-LAFR-01-1572 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-1572 ref|YP_531650.1| hypothetical protein RPC_1772 [Rhodopseudomonas ...palustris BisB18] gb|ABD87331.1| conserved hypothetical protein [Rhodopseudomonas palustris BisB18] YP_531650.1 4.5 35% ...

  8. NCBI nr-aa BLAST: CBRC-PMAR-01-0526 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PMAR-01-0526 ref|YP_532646.1| hypothetical protein RPC_2779 [Rhodopseudomonas ...palustris BisB18] gb|ABD88327.1| hypothetical protein RPC_2779 [Rhodopseudomonas palustris BisB18] YP_532646.1 1.6 28% ...

  9. NCBI nr-aa BLAST: CBRC-PABE-17-0031 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-17-0031 ref|NP_945566.1| possible OmpA family member [Rhodopseudomonas pa...lustris CGA009] emb|CAE25657.1| possible OmpA family member [Rhodopseudomonas palustris CGA009] NP_945566.1 8e-04 35% ...

  10. NCBI nr-aa BLAST: CBRC-GGAL-04-0040 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGAL-04-0040 ref|YP_779456.1| OmpA/MotB domain protein [Rhodopseudomonas palus...tris BisA53] gb|ABJ04476.1| OmpA/MotB domain protein [Rhodopseudomonas palustris BisA53] YP_779456.1 2e-07 28% ...

  11. NCBI nr-aa BLAST: CBRC-EEUR-01-1516 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-1516 ref|NP_949454.1| conjugal transfer protein trbL [Rhodopseudomonas... palustris CGA009] emb|CAE29559.1| conjugal transfer protein trbL [Rhodopseudomonas palustris CGA009] NP_949454.1 0.035 30% ...

  12. NCBI nr-aa BLAST: CBRC-DMEL-06-0076 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DMEL-06-0076 ref|YP_782397.1| transport system permease protein [Rhodopseudomonas... palustris BisA53] gb|ABJ07417.1| transport system permease protein [Rhodopseudomonas palustris BisA53] YP_782397.1 5.4 28% ...

  13. NCBI nr-aa BLAST: CBRC-GGAL-19-0001 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGAL-19-0001 ref|YP_530427.1| 3-dehydroquinate synthase [Rhodopseudomonas palu...stris BisB18] gb|ABD86108.1| 3-dehydroquinate synthase [Rhodopseudomonas palustris BisB18] YP_530427.1 1.1 28% ...

  14. NCBI nr-aa BLAST: CBRC-ACAR-01-1102 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-1102 ref|YP_780869.1| protein of unknown function DUF1109 [Rhodopseudomonas... palustris BisA53] gb|ABJ05889.1| protein of unknown function DUF1109 [Rhodopseudomonas palustris BisA53] YP_780869.1 3.5 23% ...

  15. NCBI nr-aa BLAST: CBRC-ETEL-01-1481 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ETEL-01-1481 ref|YP_484687.1| Chromosome segregation protein SMC [Rhodopseudomonas... palustris HaA2] gb|ABD05776.1| Chromosome segregation protein SMC [Rhodopseudomonas palustris HaA2] YP_484687.1 4.8 29% ...

  16. NCBI nr-aa BLAST: CBRC-ACAR-01-0693 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0693 ref|NP_946813.1| possible chloride channel [Rhodopseudomonas palu...stris CGA009] emb|CAE26907.1| possible chloride channel [Rhodopseudomonas palustris CGA009] NP_946813.1 0.41 22% ...

  17. NCBI nr-aa BLAST: CBRC-DDIS-04-0046 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DDIS-04-0046 ref|YP_487907.1| hypothetical protein RPB_4309 [Rhodopseudomonas ...palustris HaA2] gb|ABD08996.1| conserved hypothetical protein [Rhodopseudomonas palustris HaA2] YP_487907.1 2e-24 36% ...

  18. NCBI nr-aa BLAST: CBRC-TSYR-01-1170 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TSYR-01-1170 ref|NP_945550.1| hypothetical protein RPA0197 [Rhodopseudomonas p...alustris CGA009] emb|CAE25641.1| possible ABC transporter permeases [Rhodopseudomonas palustris CGA009] NP_945550.1 0.021 27% ...

  19. NCBI nr-aa BLAST: CBRC-DSIM-01-0060 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DSIM-01-0060 ref|YP_779660.1| MATE efflux family protein [Rhodopseudomonas pal...ustris BisA53] gb|ABJ04680.1| MATE efflux family protein [Rhodopseudomonas palustris BisA53] YP_779660.1 0.005 28% ...

  20. NCBI nr-aa BLAST: CBRC-DYAK-06-0065 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DYAK-06-0065 ref|NP_946413.1| hypothetical protein RPA1062 [Rhodopseudomonas p...alustris CGA009] emb|CAE26505.1| conserved hypothetical protein [Rhodopseudomonas palustris CGA009] NP_946413.1 0.002 24% ...

  1. NCBI nr-aa BLAST: CBRC-OLAT-26-0102 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-26-0102 ref|YP_782770.1| phosphate acetyltransferase [Rhodopseudomonas pa...lustris BisA53] gb|ABJ07790.1| phosphate acetyltransferase [Rhodopseudomonas palustris BisA53] YP_782770.1 0.040 30% ...

  2. NCBI nr-aa BLAST: CBRC-ACAR-01-0548 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0548 ref|NP_948279.1| NADH dehydrogenase subunit M [Rhodopseudomonas p...alustris CGA009] emb|CAE28379.1| NADH-ubiquinone dehydrogenase chain M [Rhodopseudomonas palustris CGA009] NP_948279.1 3.6 26% ...

  3. NCBI nr-aa BLAST: CBRC-DMEL-06-0076 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DMEL-06-0076 ref|YP_569610.1| transport system permease protein [Rhodopseudomonas... palustris BisB5] gb|ABE39709.1| transport system permease protein [Rhodopseudomonas palustris BisB5] YP_569610.1 1.8 29% ...

  4. NCBI nr-aa BLAST: CBRC-TSYR-01-1170 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TSYR-01-1170 ref|YP_483908.1| hypothetical protein RPB_0286 [Rhodopseudomonas ...palustris HaA2] gb|ABD04997.1| Protein of unknown function DUF214 [Rhodopseudomonas palustris HaA2] YP_483908.1 0.18 26% ...

  5. NCBI nr-aa BLAST: CBRC-TGUT-25-0000 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-25-0000 ref|YP_783714.1| NUDIX hydrolase [Rhodopseudomonas palustris BisA...53] gb|ABJ08734.1| NUDIX hydrolase [Rhodopseudomonas palustris BisA53] YP_783714.1 3.5 34% ...

  6. NCBI nr-aa BLAST: CBRC-XTRO-01-2832 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-2832 ref|NP_945901.1| hypothetical protein RPA0548 [Rhodopseudomonas p...alustris CGA009] emb|CAE25992.1| Protein of unknown function UPF0118 [Rhodopseudomonas palustris CGA009] NP_945901.1 1.5 30% ...

  7. NCBI nr-aa BLAST: CBRC-LAFR-01-1714 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-1714 ref|YP_783685.1| periplasmic sensor hybrid histidine kinase [Rhodopseudomonas... palustris BisA53] gb|ABJ08705.1| periplasmic sensor hybrid histidine kinase [Rhodopseudomonas palustris BisA53] YP_783685.1 1.8 33% ...

  8. NCBI nr-aa BLAST: CBRC-XTRO-01-0524 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0524 ref|YP_534270.1| 2-keto-3-deoxygluconate permease [Rhodopseudomonas... palustris BisB18] gb|ABD89951.1| 2-keto-3-deoxygluconate permease [Rhodopseudomonas palustris BisB18] YP_534270.1 1e-137 76% ...

  9. NCBI nr-aa BLAST: CBRC-SARA-01-1393 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-1393 ref|NP_946691.1| hypothetical protein RPA1341 [Rhodopseudomonas p...alustris CGA009] emb|CAE26784.1| conserved hypothetical protein [Rhodopseudomonas palustris CGA009] NP_946691.1 2.4 28% ...

  10. NCBI nr-aa BLAST: CBRC-DYAK-06-0053 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DYAK-06-0053 ref|NP_949396.1| hypothetical protein RPA4060 [Rhodopseudomonas p...alustris CGA009] emb|CAE29501.1| hypothetical protein [Rhodopseudomonas palustris CGA009] NP_949396.1 0.55 27% ...

  11. NCBI nr-aa BLAST: CBRC-DYAK-01-0018 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DYAK-01-0018 ref|YP_530531.1| major facilitator superfamily MFS_1 [Rhodopseudomonas... palustris BisB18] gb|ABD86212.1| major facilitator superfamily MFS_1 [Rhodopseudomonas palustris BisB18] YP_530531.1 5.7 23% ...

  12. NCBI nr-aa BLAST: CBRC-OPRI-01-0197 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OPRI-01-0197 ref|YP_001990157.1| hypothetical protein Rpal_1140 [Rhodopseudomonas... palustris TIE-1] gb|ACE99681.1| hypothetical protein Rpal_1140 [Rhodopseudomonas palustris TIE-1] YP_001990157.1 3.4 40% ...

  13. NCBI nr-aa BLAST: CBRC-XTRO-01-0170 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0170 ref|YP_531251.1| inner-membrane translocator [Rhodopseudomonas pa...lustris BisB18] gb|ABD86932.1| inner-membrane translocator [Rhodopseudomonas palustris BisB18] YP_531251.1 1e-120 62% ...

  14. NCBI nr-aa BLAST: CBRC-MLUC-01-0588 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MLUC-01-0588 ref|YP_779456.1| OmpA/MotB domain-containing protein [Rhodopseudomonas... palustris BisA53] gb|ABJ04476.1| OmpA/MotB domain protein [Rhodopseudomonas palustris BisA53] YP_779456.1 0.011 29% ...

  15. NCBI nr-aa BLAST: CBRC-OANA-01-2119 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-2119 ref|YP_530142.1| ABC transporter related [Rhodopseudomonas palust...ris BisB18] gb|ABD85823.1| ABC transporter related [Rhodopseudomonas palustris BisB18] YP_530142.1 5.8 42% ...

  16. NCBI nr-aa BLAST: CBRC-XTRO-01-0337 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0337 ref|YP_567194.1| inner-membrane translocator [Rhodopseudomonas pa...lustris BisB5] gb|ABE37293.1| inner-membrane translocator [Rhodopseudomonas palustris BisB5] YP_567194.1 7e-62 42% ...

  17. NCBI nr-aa BLAST: CBRC-XTRO-01-0435 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0435 ref|YP_782315.1| hypothetical protein RPE_3403 [Rhodopseudomonas ...palustris BisA53] gb|ABJ07335.1| conserved hypothetical protein [Rhodopseudomonas palustris BisA53] YP_782315.1 3e-25 24% ...

  18. NCBI nr-aa BLAST: CBRC-OANA-01-2190 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-2190 ref|YP_484804.1| diguanylate phosphodiesterase [Rhodopseudomonas ...palustris HaA2] gb|ABD05893.1| diguanylate phosphodiesterase [Rhodopseudomonas palustris HaA2] YP_484804.1 3.2 32% ...

  19. NCBI nr-aa BLAST: CBRC-XTRO-01-0435 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0435 ref|YP_533184.1| hypothetical protein RPC_3323 [Rhodopseudomonas ...palustris BisB18] gb|ABD88865.1| conserved hypothetical protein [Rhodopseudomonas palustris BisB18] YP_533184.1 4e-27 26% ...

  20. NCBI nr-aa BLAST: CBRC-FCAT-01-1212 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-1212 ref|YP_530164.1| ammonium transporter [Rhodopseudomonas palustris... BisB18] gb|ABD85845.1| ammonium transporter [Rhodopseudomonas palustris BisB18] YP_530164.1 0.024 24% ...

  1. Anthelmintic, antimicrobial, antioxidant and cytotoxic activity of Caltha palustris var. alba Kashmir, India.

    Science.gov (United States)

    Mubashir, Sofi; Dar, Mohd Yousuf; Lone, Bashir A; Zargar, M Iqbal; Shah, Wajaht A

    2014-08-01

    The methanolic extract obtained from the root portion of Caltha palustris var. alba was evaluated for its anthelmintic efficacy against gastrointestinal nematodes of sheep under both in vitro and in vivo conditions using worm motility inhibition (WMI) assay and fecal egg count reduction (FECR) assay, respectively. The extract was subjected to antimicrobial activity using agar-well diffusion method against different bacterial strains. In addition the extract was evaluated for cytotoxic and antioxidant activity against cultured THP-1(Leukemia), A-549 (Lung), HCT-15 (Colon), Cervix (HeLa) and PC-3(Prostrate) cell lines by SRB and DPPH radical scavenging assays. The extract used resulted in mean %WMI of 94.44%, as observed when the worms were put in lukewarm buffer for 30 min after exposure to different treatments. The mean mortality index of the sample was 0.95. The lethal concentration (LC50) was 0.11 mg·mL(-1). Cell lines were exposed to concentration of 100 μg·mL(-1) of extract for 48 h, which reduced the viability of these cell lines. The same plant extract also showed 55.58% DPPH radical scavenging activity.

  2. The neurobiology and behavior of the American water shrew (Sorex palustris).

    Science.gov (United States)

    Catania, Kenneth C

    2013-06-01

    American water shrews (Sorex palustris) are aggressive predators that dive into streams and ponds to find prey at night. They do not use eyesight for capturing fish or for discriminating shapes. Instead they make use of vibrissae to detect and attack water movements generated by active prey and to detect the form of stationary prey. Tactile investigations are supplemented with underwater sniffing. This remarkable behavior consists of exhalation of air bubbles that spread onto objects and are then re-inhaled. Recordings for ultrasound both above and below water provide no evidence for echolocation or sonar, and presentation of electric fields and anatomical investigations provide no evidence for electroreception. Counts of myelinated fibers show by far the largest volume of sensory information comes from the trigeminal nerve compared to optic and cochlear nerves. This is in turn reflected in the organization of the water shrew's neocortex, which contains two large somatosensory areas and much smaller visual and auditory areas. The shrew's small brain with few cortical areas may allow exceptional speed in processing sensory information and producing motor output. Water shrews can accurately attack the source of a water disturbance in only 50 ms, perhaps outpacing any other mammalian predator.

  3. A new heterolobosean amoeba Solumitrus palustris n. g., n. sp. isolated from freshwater marsh soil.

    Science.gov (United States)

    Anderson, O Roger; Wang, Wen; Faucher, Sebastien P; Bi, Keran; Shuman, Howard A

    2011-01-01

    During the course of research on the bacterial feeding behavior and resistance of amoebae to virulent pathogens, we isolated a new strain of amoeba from organic rich soil at the margin of freshwater swamp in the northeastern United States. Light microscopic morphology is characteristically heterolobosean, resembling vahlkampfiids, including a broadened, limax shape, and eruptive locomotion, but occasionally becoming more contracted and less elongated with lateral or anterior bulges and somewhat branching sparse, uroidal filaments. Electron microscopic evidence, including mitochondria with flattened cristae surrounded by rough endoplasmic reticulum, further indicates a heterolobosean affinity. The solitary nucleus contains a centrally located nucleolus. Cysts are rounded with occasionally an eccentrically located nucleus. The cyst walls are relatively thin, becoming crenated, and loosely enclosing the cyst when mature. Molecular genetic evidence places this isolate among the Heterolobosea, branching most closely in a clade including Allovahlkampfia spelaea and previously isolated, un-named strains of soil amoebae. Based on differentiated features, including morphology of the uroid, cyst wall structure, and molecular genetic evidence that distinguish it from A. spelaea, a new genus and species, Solumitrus palustris, is proposed for this new heterolobosean.

  4. Cloning and sequencing of complete -crystallin cDNA from embryonic lens of Crocodylus palustris

    Indian Academy of Sciences (India)

    Raman Agrawal; Reena Chandrashekhar; Anurag Kumar Mishra; Jetty Ramadevi; Yogendra Sharma; Ramesh K Aggarwal

    2002-06-01

    -Crystallin is a taxon-specific structural protein found in eye lenses. We present here the cloning and sequencing of complete -crystallin cDNA from the embryonic lens of Crocodylus palustris and establish it to be identical to the -enolase gene from non-lenticular tissues. Quantitatively, the -crystallin was found to be the least abundant crystallin of the crocodilian embryonic lenses. Crocodile -crystallin cDNA was isolated by RT-PCR using primers designed from the only other reported sequence from duck and completed by 5′- and 3′-rapid amplification of cDNA ends (RACE) using crocodile gene specific primers designed in the study. The complete -crystallin cDNA of crocodile comprises 1305 bp long ORF and 92 and 409 bp long untranslated 5′-and 3′-ends respectively. Further, it was found to be identical to its putative counterpart enzyme -enolase, from brain, heart and gonad, suggesting both to be the product of the same gene. The study thus provides the first report on cDNA sequence of -crystallin from a reptilian species and also re-confirms it to be an example of the phenomenon of gene sharing as was demonstrated earlier in the case of peking duck. Moreover, the gene lineage reconstruction analysis helps our understanding of the evolution of crocodilians and avian species.

  5. Toxicity of sulfate and chloride to early life stages of wild rice (Zizania palustris).

    Science.gov (United States)

    Fort, Douglas J; Mathis, Michael B; Walker, Rachel; Tuominen, Lindsey K; Hansel, Mike; Hall, Scott; Richards, Robin; Grattan, S R; Anderson, Kurt

    2014-12-01

    Despite the importance of wild rice (Zizania palustris) in the Great Lakes region of North America, its sensitivity to sulfate is not well understood. A 21-d hydroponic experiment was performed to determine the toxicity of sulfate to wild rice seeds and seedlings. Effects of 6 sulfate concentrations ranging from 10 mg/L to 5000 mg/L and of chloride salts at equivalent conductivity were evaluated to determine whether adverse effects were attributable to sulfate or to conductivity-related stress. Sulfate treatment decreased root length, shoot length, and leaf number, and increased phytotoxic effects at concentrations of 5000 mg/L relative to a 50 mg/L control. The time to 30% mesocotyl emergence decreased at 2500 mg/L sulfate, indicating a potential stimulatory effect. Sulfate exposures of ≤ 5000 mg/L had no effect on 5 additional end points. Multiple regression analysis indicated that most observed changes could be attributed to conductivity-related stress rather than sulfate per se, with the exception of shoot length and leaf number. Chloride was more toxic than sulfate, as determined by root length and phytotoxicity. In summary, sulfate concentrations below 5000 mg/L did not adversely affect early-life stage wild rice during a 21-d period, and effects at 5000 mg/L sulfate were attributable to conductivity-related stress rather than sulfate toxicity in 2 of 4 end points.

  6. Stenochlaena palustris aqueous extract reduces hepatic peroxidative stress in Marmota caligata with induced fever

    Directory of Open Access Journals (Sweden)

    Adenan

    2010-12-01

    Full Text Available The kelakai fern (Stenochlaena palustris contains bioactive substances, such as flavonoids, steroids, and alkaloids. The kelakai is used by the Kenyah Dayak communities for the treatment of anemia, fever, and cutaneous disorders. Fever is increased temperature regulation of the body, which process indirectly increases the production of peroxides, leading to peroxidative stress. The aim of this research study was to evaluate the influence of an aqueous extract of the kelakai fern on peroxidative stress in the hoary marmot (Marmota caligata with experimentally-induced fever. This was a true experimental study, with post test-only and control group design, involving 6 groups, each made up of 4 animals. Peroxide concentration was measured by a modification of the FOX2 method and peroxidase activity by the Kanehira method. The kelakai extract was administered to the animals by gavage at weight-adjusted dosages between 7.75 x 10-4 mg/kg body weight to 1.24 x 10-2 mg/kg body weight. Data were analyzed using singe linear regression test. The results showed that kelakai extract dose significantly reduced the peroxide levels, regression equation with the Y = 2.513-106.03 X (R2 = 0.8057, indicating that 80.5% variation of peroxide levels is predicted by the kelakai extract dose. From the results we conclude that kelakai aqueous extract decreases peroxide levels in hoary marmots with experimentally-induced fever.

  7. Aptitude of Lymnaea palustris and L. stagnalis to Fasciola hepatica larval development through the infection of several successive generations of 4-mm-high snails.

    Science.gov (United States)

    Vignoles, P; Rondelaud, D; Dreyfuss, G

    2016-06-01

    Bimiracidial infections of Lymnaea palustris and Lymnaea stagnalis (shell height at exposure, 4 mm) with Fasciola hepatica were carried out during six successive snail generations to determine if prevalence and intensity of snail infection increased over time through descendants issuing from eggs laid by parents already exposed to this digenean. Controls were constituted by a French population of Galba truncatula (a single generation) infected according to the same protocol. In a first experiment performed with the F1 to F5 generations of L. palustris, the prevalence and intensity of F. hepatica infection in snails progressively increased. Immature rediae and a few cercariae-containing rediae of the digenean were observed in L. stagnalis from the F3 generation, but no free cercaria was noted in the bodies of this lymnaeid from the F4 to F6 generations. In another experiment carried out with the F6 generation of L. palustris, the prevalence of F. hepatica infection and the number of shed cercariae were significantly lower in L. palustris than in G. truncatula. This mode of snail infection suggests an explanation for cases of human fasciolosis occurring in central France after the collection of wild watercress from beds where L. palustris was the sole lymnaeid.

  8. Multiple benefits through intelligent combination. Economic inventory optimization through the use of photovoltaics; Mehrfachnutzen durch intelligente Kombination. Wirtschaftliche Bestandsoptimierung durch Nutzung von Photovoltaik

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2013-10-15

    By installing a PV system the roof areas are additionally used for power generation. To ensure the operability permanently, they must be carefully planned, built and maintained. Based on three demonstration examples it is shown how inventory optimization can be achieved by maintaining high quality standards (tennis hall Oberthal and Ottweiler, warehouse roof of the building material dealer Lauer). [German] Durch die Installation einer PV-Anlage werden die Dachflaechen zusaetzlich zur Energiegewinnung genutzt. Um die Funktionsfaehigkeit dauerhaft zu gewaehrleisten, muessen sie sorgfaeltig geplant, gebaut und gewartet werden. Anhand von drei Demonstrationsbeispielen (Tennishalle Oberthal und Ottweiler, Lagerhallendach des Baustoffhaendlers Lauer) wird gezeigt, wie die Bestandsoptimierung unter Einhaltung hoher Qualitaetsstandards geloest werden kann.

  9. Prevalence of Food Impaction-Induced Periodontitis in Conventionally Housed Marsh Rice Rats (Oryzomys palustris).

    Science.gov (United States)

    Messer, Jonathan G; Jiron, Jessica M; Chen, Hung-Yuan; Castillo, Evelyn J; Mendieta Calle, Jorge L; Reinhard, Mary K; Kimmel, Donald B; Aguirre, J Ignacio

    2017-02-01

    Marsh rice rats (Oryzomys palustris) fed a pelleted diet high in sucrose and casein have been used as a model for moderate to severe periodontitis. Here we characterize the prevalence, location, and histopathologic features of food-impaction lesions (FIL), a unique type of oral event, in rice rats fed standard pelleted rodent chow from weaning until 34 wk of age. Healthy female rats (n = 90; age, 4 wk) were weaned into groups (n = 10 to 24) and were euthanized at 4, 16, 22, 28, or 34 wk of age. At necropsy, high-resolution photographs of the 4 jaw quadrants were examined by 3 independent observers to determine the presence, number, and location of FIL. In addition, gross periodontitis was scored (scale, 0 to 4), and the hemimaxillar surface area containing FIL was measured. Serial sections of decalcified jaws were assessed histologically. The prevalence of FIL increased with age, and was 0% (baseline), 59.1%, 69.6%, 81.8% and 80.0% in rats at age 4, 16, 22, 28, and 34 wk, respectively. FIL were predominantly located (93.9%) in the maxillary palatal surfaces of the interproximal area between molars 2 and 3 and did not affect mandibular surfaces. The percentage of the hemimaxillar surface area occupied by FIL was 6.83%, 4.82%, 2.88%, and 6.52% in rats at age 16, 22, 28, and 34 wk, respectively. Histopathologic changes in FIL varied from localized gingivitis to larger, localized periodontitis-like lesions. These data indicate that FIL are common in rice rats fed standard rodent chow, are slight to mild in severity, and are localized to specific regions in the oral cavity, thus suggesting they may be a suitable model for local maxillary periodontitis when fed standard rodent chow.

  10. Forest floor depth mediates understory vigor in xeric Pinus palustris ecosystems.

    Science.gov (United States)

    Hiers, J Kevin; O'Brien, Joseph J; Will, Rodney E; Mitchell, Robert J

    2007-04-01

    Longleaf pine (Pinus palustris) woodlands and savannas are among the most frequently burned ecosystems in the world with fire return intervals of 1-10 years. This fire regime has maintained high levels of biodiversity in terms of both species richness and endemism. Land use changes have reduced the area of this ecosystem by >95%, and inadequate fire frequencies threaten many of the remnants today. In the absence of frequent fire, rapid colonization of hardwoods and shrubs occurs, and a broad-leaved midstory develops. This midstory encroachment has been the focus of much research and management concern, largely based on the assumption that the midstory reduces understory plant diversity through direction competition via light interception. The general application of this mechanism of degradation is questionable, however, because midstory density, leaf area, and hardwood species composition vary substantially along a soil moisture gradient from mesic to extremely xeric sites. Reanalysis of recently reported data from xeric longleaf pine communities suggests that the development of the forest floor, a less conspicuous change in forest structure, might cause a decline in plant biodiversity when forests remain unburned. We report here a test of the interactions among fire, litter accumulation, forest floor development, and midstory canopy density on understory plant diversity. Structural equation modeling showed that within xeric sites, forest floor development was the primary factor explaining decreased biodiversity. The only effects of midstory development on biodiversity were those mediated through forest floor development. Boundary line analysis of functional guilds of understory plants showed sensitivity to even minor development of the forest floor in the absence of fire. These results challenge the prevailing management paradigm and suggest that within xeric longleaf pine communities, the primary focus of managed fire regime should be directed toward the

  11. Structural change by reorganisation in the coal mining industry; Strukturwandel durch Neuordnung im Steinkohlenbergbau

    Energy Technology Data Exchange (ETDEWEB)

    Beimann, W. [Montan-Grundstuecksgesellschaft mbH (MGG), Essen (Germany)

    1998-06-10

    The renewed retrenchment of the German coal mining industry on the basis of the decisions in March 1977 is changing the image of the former coal mining companies and regions. The RAG group counteracted this change at an early stage by diversification and internationalisation of its activities. However, the structural change in the area is also assisted in various ways: By investments, training in occupations with a promising future and by preparing and making available the former colliery sites. With the `Flaeche Plus` concept of MGG the RAG group is intensifying its efforts in the marketing, development and utilisation of the areas. (orig./MSK) [Deutsch] Die erneute Rueckfuehrung des deutschen Steinkohlenbergbaus aufgrund der Beschluesse vom Maerz 1997 veraendert das Bild der Steinkohlenunternehmen und -regionen. Der RAG-Konzern begegnete diesem Wandel fruehzeitig durch eine Diversifizierung und Internationalisierung der Aktivitaeten. Aber auch der Strukturwandel im Revier wird vielfaeltig unterstuetzt: Durch Investitionen, die Ausbildung in zukunftstraechtigen Berufen und durch Aufbereitung und Zurverfuegungstellung der ehemaligen Bergbauflaechen. Mit dem Konzept `Flaeche Plus` der MGG bemueht sich der RAG-Konzern auch intensiv um die Vermarktung, Entwicklung und Nutzung der Flaechen. (orig./MSK)

  12. Retrospektive Bestimmung der elektromagnetischen Exposition durch analoge Rundfunksender im Rahmen von KiSS

    Directory of Open Access Journals (Sweden)

    J. Philipp

    2007-06-01

    Full Text Available Im Rahmen einer epidemiologischen Fall-Kontroll-Studie zur Untersuchung eines möglichen Zusammenhangs zwischen der Häufigkeit kindlicher Leukämien und elektromagnetischer Strahlung (,,KiSS" – Kindliche Leukämien und Expositionen in der Umgebung von hochfrequenten Sendestationen soll die Exposition durch leistungsstarke analoge Rundfunksender retrospektiv (Zeitraum 1982–2003 quantifiziert werden. Die zu betrachtenden Sendernetze für AM-Hörfunk, FM-Hörfunk und analoges Fernsehen unterscheiden sich nicht nur hinsichtlich der Modulationsart und der von den Einzelsendern abgestrahlten Leistung, sondern auch in der Netzkonfiguration und den je nach Wellenbereich verschiedenen Strahlungseigenschaften der Sendeantennen. Damit sind bei diesen drei Rundfunkdiensten sowohl die absolute Größe als auch die räumliche Struktur der hervorgerufenen Exposition verschieden. Es wird dargelegt, wie die für die Prognose der Rundfunkversorgung verwendeten Rechenverfahren zur Modellierung der Feldstärke für die Modellierung der Exposition herangezogen und durch Kontrollmessungen validiert werden und wie trotz Wahrung der Vertraulichkeit der dabei unabdingbar zu verwendenden Senderbetriebsdaten eine unerwünschte Beeinflussung der Studienergebnisse durch die Senderbetreiber ausgeschlossen wird.

  13. Ecosystem engineering potential of the gastropod Terebralia palustris (Linnaeus, 1767) in mangrove wastewater wetlands - A controlled mesocosm experiment

    Energy Technology Data Exchange (ETDEWEB)

    Penha-Lopes, Gil, E-mail: gil.penha-lopes@biology-research.co [Centro de Oceanografia - Laboratorio Maritimo da Guia, Departamento de Biologia Animal, Faculdade de Ciencias da Universidade de Lisboa, Avenida Na, Senhora do Cabo 939, 2750-374 Cascais (Portugal); Department of Analytical and Environmental Chemistry, Vrije Universiteit Brussels, Brussels (Belgium); Bartolini, Fabrizio [Dipartimento di Biologia Evoluzionistica, Universita degli Studi di Firenze, via Romana 17, I-50125 Firenze (Italy); Limbu, Samwel [University of Dar es Salaam, Department of Aquatic Sciences and Fisheries, P.O. Box 35064, Dar es Salaam (Tanzania, United Republic of); Cannicci, Stefano [Dipartimento di Biologia Evoluzionistica, Universita degli Studi di Firenze, via Romana 17, I-50125 Firenze (Italy); Mgaya, Yunus [University of Dar es Salaam, Department of Aquatic Sciences and Fisheries, P.O. Box 35064, Dar es Salaam (Tanzania, United Republic of); Kristensen, Erik [Institute of Biology, University of Southern Denmark, DK-5230 Odense M (Denmark); Paula, Jose [Centro de Oceanografia - Laboratorio Maritimo da Guia, Departamento de Biologia Animal, Faculdade de Ciencias da Universidade de Lisboa, Avenida Na, Senhora do Cabo 939, 2750-374 Cascais (Portugal)

    2010-01-15

    The effect of different sewage concentrations (0, 20, 60 and 100%), vegetation (Bare, Avicennia marina or Rhizophora mucronata) and immersion periods (immersion/emersion period of 12/12 h or 3/3 days just for 100%) conditions were studied for 6 months on survival and growth rates of Terebralia palustris (Linnaeus, 1767). Gastropods' activity and ecosystem engineering preformed at bare and A. marina planted cells and 3 sewage conditions (0, 20 and 60%) were determined. Survival rates were higher than 70% in all treatments. Growth rate decreased significantly with increasing sewage concentrations (mainly at unplanted conditions) and longer immersion periods. A complete shift (from immersion to emersion periods) and a significant decrease in mobility and consequently its engineer potential, due to sewage contamination, lead to a 3-4 fold decrease in the amount of sediment disturbed. Sewage contamination, primary producers' abundance and environmental conditions may have influenced the gastropods survival, growth and its ecosystem engineering potential. - Terebralia palustris high ecosystem engineering potential in constructed mangrove wetlands.

  14. Hydrogen gas production by fermentation from various organic wastewater using Clostridium butyricum NCIB 9576 and Rhodopseudomonas sphaeroides E15-1

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Young Sue; Kim, Hyun Kyung; Rye, Hye Yeon; Lee, In Gu; Kim, Mi Sun [Biomass Research Team, Korea Institute of Energy Research, Taejeon (Korea)

    2000-03-01

    Anaerobic fermentation using Clostidium butyricum NCIB 9576, and phto-fermentation using Rhodopseudomonas sphaeroides E15-1 were studied for the production of hydrogen from Makkoli, fruits (orange and apple, watermelon and melon) and Tofu wastewaters. From the Makkoli wastewater, which contained 0.94 g/liter sugars and 2.74 g/liter solubel starch, approximately 49 mM H{sub 2}/liter wastewater was produced during the initial 18h of the anaerobic fermentation with pH control between 6.5-7.0. Several organic acids such as butyric acid, acetic acid, propionic acid, lactic acid and ethanol were also produced. From watermelon and melon wastewater, which contained 43 g/liter sugars, generated about approximately 71 mM H{sub 2}/liter wastewater was produced during the initial 24h of the anaerobic fermentation. Tofu wastewater, pH 6.5, containing 12.6 g/liter soluble starch and 0.74 g/liter sugars, generated about 30mM H{sub 2}/liter wastewater, along with some organic acids, during the initial 24 h of anaerobic fermentation. Makkoli and Tofu wastewaters as substrates for the photo-fermentation by Rhodopseudomonas sphaeroides E15-1 produced approximately 37.9 and 22.2 {mu}M H{sub 2}/ml wastewaters, respectively for 9 days of incubation under the average of 9,000010,000 lux illumination at the surface of reactor using tungsten halogen lamps. Orange and apple wastewater, which contained 93.4 g/l produced approximately 13.1 {mu}M H{sub 2}/ml wastewater only for 2 days of photo-fermentation and the growth of Rhodopseudomonas spnaeroides E15-1 and hydrogen production were stopped. 22 refs, 4 figs., 2 tabs.

  15. Synchrotron Small-Angle X-Ray Scattering Investigation on Integral Membrane Protein Light-Harvesting Complex LH2 from Photosynthetic Bacterium Rhodopseudomonas Acidophila

    Institute of Scientific and Technical Information of China (English)

    DU Lu-Chao; WENG Yu-Xiang; HONG Xin-Guo; XIAN Ding-Chang; Kobayashi Katsumi

    2006-01-01

    @@ Structures of membrane protein in solution are different from that in crystal phase. We present the primary results of small angle x-ray scattering (SAXS) resolved topological structures of a light harvesting antenna membrane protein complex LH2 from photosynthetic bacteria Rhodopseudomonas acidophila in detergent solution for the first time. Our results show that the elliptical shape of the LH2 complex in solution clearly deviates from its circular structure in crystal phase determined by x-ray diffraction. This result provides an insight into the structure and function interplay in LH2.

  16. Effects of drought and shade on growth and water use of Quercus alba, Q. bicolor, Q. imbricaria and Q. palustris seedlings

    Science.gov (United States)

    Joseph J. McCarthy; Jeffrey O. Dawson

    1991-01-01

    Growth and water use efficiency were determined for 2-year-old white oak (Quercus alba), swamp white oak (Q. imbricaria) and pin oak (Q. palustris) seedlings grown under three shade treatments (30, 55 and 73%) and two irrigation regimes (container capacity and mild drought). With species and water regimes...

  17. H2 metabolism in the photosynthetic bacterium Rhodopseudomonas capsulata: production and utilization of H2 by resting cells.

    Science.gov (United States)

    Hillmer, P; Gest, H

    1977-02-01

    Photoproduction of H2 and activation of H2 for CO2 reduction (photoreduction) by Rhodopseudomonas capsulata are catalyzed by different enzyme systems. Formation of H2 from organic compounds is mediated by nitrogenase and is nto inhibited by an atmosphere of 99% H2. Cells grown photoheterotrophically on C4 dicarboxylic acids (with glutamate as N source) evolve H2 from the C4 acids and also from lactate and pyruvate; cells grown on C3 carbon sources, however, are inactive with the C4 acids, presumably because they lack inducible transport systems. Ammonia is known to inhibit N2 fixation by photosynthetic bacteria, and it also effectively prevents photoproduction of H2; these effects are due to inhibition and, in part, inactivation of nitrogenase. Biosynthesis of the latter, as measured by both H2 production and acetylene reduction assays, is markedly increased when cells are grown at high light intensity; synthesis of the photoreduction system, on the other hand, is not appreciably influenced by light intensity during photoheterotrophic growth. The photoreduction activity of cells grown on lactate + glutamate (which contain active nitrogenase) is greatly activated by NH4+, but this effect is not observed in cells grown with NH4+ as N source (nitrogenase repressed) or in a Nif- mutant that is unable to produce H2. Lactate, malate, and succinate, which are readily used as growth substrates by R. capsulata and are excellent H donors for photoproduction of H2, abolish photoreduction activity. The physiological significances of this phenomenon and of the reciprocal regulatory effects of NH4+ on H2 production and photoreduction are discussed.

  18. Innovation im Mittelstand am Beispiel Der Senkung Von Mobilitätskosten Durch Schwarmintelligenz"

    Science.gov (United States)

    Fischer, Hieronymus

    Die zunehmende Vernetzung von Einzelsystemen im Fahrzeug wird durch den Einsatz weiterer Kommunikationsverbindungen (CAR2X-Kommunikation) zu einer hierarchischen Struktur führen, die den Austausch von Informationen zwischen einer Vielzahl von verteilten heterogenen Subsystemen auf mobilen und stationären Plattformen ermöglicht. Die primären Ziele einer solchen Vernetzung von Cyber-Physical Systems sind die Bereitstellung neuer Dienste im Fahrzeug, die Erhöhung der Verkehrssicherheit sowie die effektive Steuerung der weiter wachsenden Verkehrsströme.

  19. Effects of landfills on the limnology of associated running waters; Gefaehrdung von Oberflaechengewaessern durch Altablagerungen

    Energy Technology Data Exchange (ETDEWEB)

    Baier, B. [Hohenheim Univ., Stuttgart (Germany). Inst. fuer Zoologie; Kappus, B. [Hohenheim Univ., Stuttgart (Germany). Inst. fuer Zoologie; Boehmer, J. [Hohenheim Univ., Stuttgart (Germany). Inst. fuer Zoologie; Nill, A. [Hohenheim Univ., Stuttgart (Germany). Inst. fuer Zoologie; Rahmann, H. [Hohenheim Univ., Stuttgart (Germany). Inst. fuer Zoologie

    1997-10-01

    Five small brooks influenced by runoff from landfills were surveyed from May 1993 to March 1994 using chemical and biological methods. Results showed clear impacts on the macroinvertebrate fauna as a result of pollutants in three of the five brooks studied. (orig.) [Deutsch] Im Einflussbereich von Altdeponien wurden fuenf kleine Fliessgewaesser von Mai 1993 bis Maerz 1994 wasserchemisch und biologisch untersucht. Anhand der Untersuchungsergebnisse wurde eine Gefaehrdungsabschaetzung der Oberflaechengewaesser durch die Altablagerungen vorgenommen. Danach waren bei drei der fuenf untersuchten Deponien aufgrund von Schadstoffeintraegen Auswirkungen auf die Makrobenthosfauna der angrenzenden Fliessgewaesser erkennbar. (orig.)

  20. Anabole Wirkungen auf den Knochen durch pulsatiles exogenes Parathormon - ein wiederentdecktes Therapieprinzip

    Directory of Open Access Journals (Sweden)

    Bernecker PM

    2002-01-01

    Full Text Available Parathormon (PTH wird in den Zellen der Nebenschilddrüse produziert und in enger Abhängigkeit vom Serumspiegel des ionisierten Kalziums in die Zirkulation abgegeben. Ein Abfall des Serum-Kalziums führt zu einem augenblicklichen Ansteigen der PTH-Ausschüttung aus der Nebenschilddrüse, ohne daß "second messenger"-Mechanismen zum Tragen kommen; viel mehr wird die Kalziumkonzentration durch einen auf der Oberfläche der Nebenschilddrüsenzellen befindlichen Kalzium-empfindlichen Rezeptor direkt durch die Zelle erfaßt. PTH wirkt auf verschiedene Weise; Ziel der Wirkung ist in jedem Fall eine Anhebung des Serum-Kalzium-Spiegels. Aus der Extrazellulärflüssigkeit (ECF des Knochens, die durch das Havers'sche Kanalsystem zirkuliert, kann recht rasch Kalzium mobilisiert werden. In der Knochen-ECF sind ca. 1-3 Gramm Kalzium in lockerer Ionenbindung gespeichert; diese können unter dem Einfluß von Parathormon sehr rasch in das Blutkompartment übergeführt werden. Am Knochen stimuliert Parathormon die Knochenresorption, auch hier mit dem physiologischen Ziel, Kalzium freizusetzen. Da der Knochenstoffwechsel jedoch in definierten "Knochenumbaueinheiten" (bone remodelling units = BMU erfolgt, in denen Knochenresorption und Knochenformation eng gekoppelt sind und sequentiell ablaufen, wurde die Steuerung dieser Umbauvorgänge durch PTH schon vor längerer Zeit untersucht. Die Überraschung war groß, als man feststellen mußte, daß die den Knochen abbauenden Osteoklasten keinerlei Rezeptoren für PTH, sondern nur für dessen physiologischen Gegenspieler, das Calcitonin, besitzen. PTH-Rezeptoren wurden dagegen in großer Dichte auf Osteoblasten, also Knochen-aufbauenden Zellen, gefunden. Es wird daher angenommen, daß der initiale Reiz für die Knochenresorption auf die Osteoklasten indirekt übermittelt wird, und zwar über bisher unbekannte second-messaging-Mechanismen; Prostaglandine der E- und F-Serien als auch Interleukin-1 wurden hier als solche

  1. Population structure, density and food sources of Terebralia palustris (Potamididae: Gastropoda) in a low intertidal Avicennia marina mangrove stand (Inhaca Island, Mozambique)

    Science.gov (United States)

    Penha-Lopes, Gil; Bouillon, Steven; Mangion, Perrine; Macia, Adriano; Paula, José

    2009-09-01

    Population structure and distribution of Terebralia palustris were compared with the environmental parameters within microhabitats in a monospecific stand of Avicennia marina in southern Mozambique. Stable carbon and nitrogen isotope analyses of T. palustris and potential food sources (leaves, pneumatophore epiphytes, and surface sediments) were examined to establish the feeding preferences of T. palustris. Stable isotope signatures of individuals of different size classes and from different microhabitats were compared with local food sources. Samples of surface sediments 2.5-10 m apart showed some variation (-21.2‰ to -23.0‰) in δ13C, probably due to different contributions from seagrasses, microalgae and mangrove leaves, while δ15N values varied between 8.7‰ and 15.8‰, indicating that there is a very high variability within a small-scale microcosm. Stable isotope signatures differed significantly between the T. palustris size classes and between individuals of the same size class, collected in different microhabitats. Results also suggested that smaller individuals feed on sediment, selecting mainly benthic microalgae, while larger individuals feed on sediment, epiphytes and mangrove leaves. Correlations were found between environmental parameters and gastropod population structure and distribution vs. the feeding preferences of individuals of different size classes and in different microhabitats. While organic content and the abundance of leaves were parameters that correlated best with the total density of gastropods (>85%), the abundance of pneumatophores and leaves, as well as grain size, correlated better with the gastropod size distribution (>65%). Young individuals (height < 3 cm) occur predominantly in microhabitats characterized by a low density of leaf litter and pneumatophores, reduced organic matter and larger grain size, these being characteristic of lower intertidal open areas that favour benthic microalgal growth. With increasing shell

  2. Etablierung eines Tiermodells des hepatozellul(a)ren Karzinoms durch den Einsatz von allogenischer Implantationstechnik

    Institute of Scientific and Technical Information of China (English)

    QIAN; Jun; FENG; Gansheng; Trübenbach; J; Huppert; P; E; Pereira; P; L; Claussen; C; D

    2001-01-01

    Etablierung eines tierexperimentellen Modells des hepatozellularen Karzinoms(HCC) bei ACI-Ratten durch den Einsatz von allogenischer Implantationstechnik und Beobachtung durch die MR-Untersuchung. Bei einer mannlichen ACI-Ratte wurde die Tumorzellen yon Morris Hepatoma 3924A in die Flanke subkutan injiziert. 13 Tage danach erfolgte die Implantation eines soliden Tumorfragments (1 mm3) aus dem gewachsenen Tumor subkapsular in den linkem Leberlappen bei anderen mannlichen ACI-Ratten (n= 12). 14 Tage nach Implantation wurden die Tumorvolumina kernspintomographisch bestimmt. 14 Tage nach Implantation wurde der isolierte und solide Lebertumor bei allen ACI-Ratten kernspintomographisch beobachtet. Das durchschnittliche Tumorvolumen betrug hierbei 0,090± 0,008 cm3. Die Angehrate der Tumorimplantation war 100 %. Eine auffallende Hypointensitat der Lasion im Vergleich zum umgebenden Lebergewebe im Tlw-Bild und eine massig vermehrte Signalintensitat im T2w-Bild mit inhomogener Struktur waren bei allen implantierten Ratten erkennbar. Mit einer guten Angehrate des Implantations und geeignetem Tumorwachstumsverhalten steht dieses Verfahren zur Etablierung eines Tiermodells des HCCs zur Verfugung. Es ist einfach zu kontrollieren und leicht reproduzierbar.

  3. Intensifying enhanced biological phosphorus removal by calciumdolomitehydrate; Intensivierung der vermehrten biologischen Phosphatelimination durch Dolomitkalkhydrat

    Energy Technology Data Exchange (ETDEWEB)

    Soelter, M. [AKDOLIT GmbH, Wuelfrath (Germany)

    1999-02-01

    Utilization of enhanced biological phosphorus removal is increasingly considered an essential part in designing wastewater treatment plant due to ecological and economical advantages. Effects of Akdophos (=special dolime-hydrate) were investigated and monitored in operational tests at the industrial effluent clarification plant of Gerolsteiner Brunnen and the municipal plant Dremmen. Results of a full year cyclus demonstrate the efficiency of Akdophos in stabilizing and intensifying the enhanced Bio-P removal. Cost-analysis shows a considerable cost cutting potential regarding precipitants as well as improvement of sludge volume index with only low increase of excess sludge. Dry product dosage of Akdophos to the aeration tank has been proved a reliable and cost-effective process method. (orig.) [Deutsch] Oekokogische und oekonomische Vorteile bestaetigen zunehmend die Nutzung der erweiterten biologischen Phosphatelimination als wesentlichen Bestandteil der Konzeption von Abwasserreinigungsanlagen. Anhand grosstechnischer Versuche auf der Klaeranlage des Gerolsteiner Brunnen und auf der Klaeranlage Dremmen (Stadt Heinsberg) wurde die Wirkung von Akdorphos (=speziell hergestelltes Dolomitkalkhydrat) auf die biologischen Vorgaenge von Klaeranlagen untersucht. Im Betrieb ueber einen vollstaendigen Jahreszyklus konnte nachgewiesen werden, dass die biologische Phosphatelimination durch eine geringfuegige Dosierung von Akdophos intensiviert und stabilisiert wird. Die Kostenanalyse in beiden betrachteten Faellen zeigt neben einer Kostenreduktion bei den Faellmitteln weitere Einsparungen durch deutlich verbesserte Schlammabsetzeigenschaften bei sehr geringem Schlammehranfall. Die Dosierung von Akdophos erfolgt vorzugsweise trocken und unmittelbar in die Belebung. Die entsprechende Anlagentechnik hat sich als betriebssicher und kostenguenstig erwiesen. (orig.)

  4. Ecosystem engineering potential of the gastropod Terebralia palustris (Linnaeus, 1767) in mangrove wastewater wetlands--a controlled mesocosm experiment.

    Science.gov (United States)

    Penha-Lopes, Gil; Bartolini, Fabrizio; Limbu, Samwel; Cannicci, Stefano; Mgaya, Yunus; Kristensen, Erik; Paula, José

    2010-01-01

    The effect of different sewage concentrations (0, 20, 60 and 100%), vegetation (Bare, Avicennia marina or Rhizophora mucronata) and immersion periods (immersion/emersion period of 12/12 h or 3/3 days just for 100%) conditions were studied for 6 months on survival and growth rates of Terebralia palustris (Linnaeus, 1767). Gastropods' activity and ecosystem engineering preformed at bare and A. marina planted cells and 3 sewage conditions (0, 20 and 60%) were determined. Survival rates were higher than 70% in all treatments. Growth rate decreased significantly with increasing sewage concentrations (mainly at unplanted conditions) and longer immersion periods. A complete shift (from immersion to emersion periods) and a significant decrease in mobility and consequently its engineer potential, due to sewage contamination, lead to a 3-4 fold decrease in the amount of sediment disturbed. Sewage contamination, primary producers' abundance and environmental conditions may have influenced the gastropods survival, growth and its ecosystem engineering potential.

  5. Temperature-induced dissociation reaction and dynamics of light-harvesting complex Ⅱ isolated from purple photosynthetic bacterium Rps. palustris

    Institute of Scientific and Technical Information of China (English)

    FENG Juan; LI XueFeng; LIU Yuan

    2007-01-01

    Steady-state absorption spectroscopy, circular dichroism, and resonance Raman spectroscopy have been used to investigate the thermal stability of LH2 complex isolated from purple photosynthetic bacterium Rps. Palustris. The results show that: 1) upon increasing the temperature, a transition from B800 and B850 to free bacteriochlorophyll (B780) happens; 2) a gradual decrease and disappearance of CD signal in visible region occur; 3) a shift of the frequency, belonging to C=C and C-C stretching vibration, to higher wavenumber takes place. It is suggested that LH2 complex can be dissociated in the presence of B800, B850 and carotenoids simultaneously. Single-exponential fitting on the dynamic decay curves gives the apparent time constants of hundreds of minutes for various pigments.

  6. Deutsch Durch Audio-Visuelle Methode: An Audio-Lingual-Oral Approach to the Teaching of German.

    Science.gov (United States)

    Dickinson Public Schools, ND. Instructional Media Center.

    This teaching guide, designed to accompany Chilton's "Deutsch Durch Audio-Visuelle Methode" for German 1 and 2 in a three-year secondary school program, focuses major attention on the operational plan of the program and a student orientation unit. A section on teaching a unit discusses four phases: (1) presentation, (2) explanation, (3)…

  7. A comparative study of the seed germination capabilities of Anacamptis palustris (Orchidaceae, a threatened terrestrial orchid, and other more common Anacamptis species, by asymbiotic culture in vitro

    Directory of Open Access Journals (Sweden)

    Sara Magrini

    2012-02-01

    Full Text Available The increasing emphasis on terrestrial orchid conservation has led to conservation actions for a wide range of threatened Mediterranean species. Many terrestrial orchids are currently at great risk for extinction as a result of a multiplicity of threatening processes. We focus on orchid seed germination capabilities in vitro, specifically germination capability of a threatened species, Anacamptis palustris, compared to three other more common species (A. laxiflora, A. morio, and A. papilionacea, and also discuss its potential impact on orchid distribution and conservation. Asymbiotic germination tests were performed with mature seeds using BM-1 medium. In vitro seed germination and protocorm developmental stages were evaluated up to 20 weeks after sowing. Significant differences in seedling development were detected among the species, and a correlation was evident between the rarity of the species and their germinability. Thus, the presence of intrinsic, biological factors that affect and limit the distribution of A. palustris may exist.

  8. Value-added services through professional innovation management; Mehrwert durch professionelles Innovationsmanagement

    Energy Technology Data Exchange (ETDEWEB)

    Niemeier, D. [Accenture GmbH, Frankfurt am Main (Germany); Scholtissek, S.

    2001-02-01

    Competition in the deregulated internal market in electricity has changed this market into a commodity market. All traders in the energy sector today are looking for innovative marketing and sales strategies linked to the product 'electric power' and the related services. Cooperation within the framework of a multi-utility enterprise is a key example in this context. The article discusses successful business management strategies. (orig./CB) [German] Wettbewerb hat den Energiemarkt in einen Commodity-Markt verwandelt. Alle Energieversorger arbeiten heute an den gleichen Ideen wie energienahe Dienstleistungen, Innovationsbeispiel Multi Utility, Powerline Communication oder dezentrale Erzeugung. Damit diese Produkte schnell dem Kunden zur Verfuegung stehen, bedarf es einer Professionalisierung des Innovationsmanagements durch klar definierte Strategien, kreatives organisatorisches und effizientes Wissensmanagement sowie flexible Start-up-Strukturen. (orig./CB)

  9. Die Stadt tanzt! Über den Prozess der Raumproduktion durch illegale Partys in Berlin

    Directory of Open Access Journals (Sweden)

    Chantal Remmert

    2014-10-01

    Full Text Available Wer an einem sonnigen Sonntagnachmittag durch die nahe Peripherie Berlins streift, hat große Chancen, auf eine unangemeldete, sogenannte illegale Party zu stoßen. Diese werden von unterschiedlichen Akteur_innen veranstaltet und besucht, die sich aktiv an einem Prozess der Aneignung des städtischen Raums beteiligen. Eine weitere Akteurin ist die Polizei, deren Aufgabe ist es, die Partys aufzulösen und zu unterbinden. Die Interaktion der verschiedenen Akteur_innen sowie ihre Rolle und Wirkung im Prozess der Entstehung, der Durchführung und Auflösung dieser räumlichen Situation sollen hier näher untersucht werden. Die These, dass illegale Partys Prozesse der Raumproduktion mit subversivem Potenzial sind, stützt sich auf empirische Beobachtungen sowie auf theoretische Überlegungen.

  10. Pretreatment of excess sludge by electroporation; Vorbehandlung von Ueberschussschlamm durch Elektroporation

    Energy Technology Data Exchange (ETDEWEB)

    Kopplow, O.; Barjenbruch, M. [Univ. Rostock, Rostock (Germany); Heinz, V. [TU Berlin, Berlin (Germany)

    2003-07-01

    Disintegration of sewage sludge destroys microorganisms and releases cell constituents, thus making carbon available more quickly for reactions. Electroporation is a high-power pulsed technology which has hardly ever been tested in Germany. The paper presents laboratory results, which are compared with other processes (high-pressure homogeniser, thermal treatment). (orig.) [German] Der anaerobe Stabilisationsprozess haengt unter anderem von der Bioverfuegbarkeit des Kohlenstoffs ab. Durch eine Vorbehandlung (Desintegration) des Schlammes kann infolge der Zerstoerung von Mikroorganismen und der Freisetzung von Zellinhaltsstoffen der Kohlenstoff mikrobiell besser und schneller umgesetzt werden. Die Elektroporation, eine Technologie der Hochleistungspulstechnik, wird als Desintegrationsverfahren in der Literatur genannt, es liegen aber kaum Ausfuehrungen dazu in Deutschland vor. Erste Ergebnisse, die in Laborversuchen mit der Elektroporation von Ueberschussschlamm gewonnen wurden, werden hier vorgestellt und mit anderer Verfahren (Hochdruckhomogenisator, thermische Behandlung) verglichen. (orig.)

  11. Die Behandlung Adolf Hitlers im Lazarett Pasewalk 1918: Historische Mythenbildung durch einseitige bzw. spekulative Pathographie

    Directory of Open Access Journals (Sweden)

    Armbruster J

    2009-01-01

    Full Text Available Das Interesse an der Person Adolf Hitlers (1889–1945 scheint auch über 60 Jahre nach seinem Tod ungebrochen. Im Zuge dessen finden sich unter pathographischen Betrachtungen auch Tendenzen, Fragmente aus dem Kontext der Gesamtbiographie herauszulösen, in ihrer Bedeutung zu überhöhen und unter Vernachlässigung der Komplexität historischer Strukturen und Prozesse zu deuten. Dies lässt sich beispielhaft an einer Reihe von Arbeiten zeigen, die sich u. a. der Behandlung einer Kriegsverletzung Hitlers 1918 im Reservelazarett Pasewalk widmen und mit spekulativen Thesen einen Status historischer Authentizität beanspruchen. Während in Bezug auf Hitlers Gesundheitszustand ein Teil überlieferter somatischer Befunde gewisse Rückschlüsse zulässt, fehlen solche Dokumente im Hinblick auf seinen psychischen Zustand. In den vergangenen Jahren rückte der Psychiater Edmund Robert Forster (1878–1933 zunehmend in den Fokus des Interesses. Auf Grundlage eines Berichtes des US-Marine-Geheimdienstes aus dem Jahre 1943 wurde die These entwickelt, dass Forster Hitler behandelt habe und sich vornehmlich aufgrund dessen 1933 suizidierte. Einige Arbeiten bauen unter Ausblendung alternativer Möglichkeiten diese Theorie auf Grundlage der Romanvorlage „Der Augenzeuge“ von Ernst Weiß (1882–1940 bis zu einer vermeintlich gesicherten Hypnosetherapie Hitlers durch Forster aus. Die Stilisierung Forsters zum „Schöpfer“ Hitlers kann angesichts des Aufgreifens durch die Medien als Beispiel für die Entwicklung eines Mythos herangezogen werden. Der Tragik des Schicksals Forsters wird auf diese Weise wenig Rechnung getragen. Gleichzeitig birgt dies die Gefahr, dass Hitler zu einem Teil von seiner Verantwortung entbunden wird. Insofern sollte eine Diagnostik ex post überhaupt nur mit größter Zurückhaltung erfolgen.

  12. Fluorescence-excitation and emission spectra from LH2 antenna complexes of Rhodopseudomonas acidophila as a function of the sample preparation conditions.

    Science.gov (United States)

    Kunz, Ralf; Timpmann, Kõu; Southall, June; Cogdell, Richard J; Köhler, Jürgen; Freiberg, Arvi

    2013-10-10

    The high sensitivity of optical spectra of pigment-protein complexes to temperature and pressure is well known. In the present study, we have demonstrated the significant influence of the environments commonly used in bulk and single-molecule spectroscopic studies at low temperatures on the LH2 photosynthetic antenna complex from Rhodopseudomonas acidophila. A transfer of this LH2 complex from a bulk-buffer solution into a spin-coated polymer film results in a 189 cm(-1) blue shift of the B850 excitonic absorption band at 5 K. Within the molecular exciton model, the origin of this shift could be disentangled into three parts, namely to an increase of the local site energies, a contraction of the exciton band, and a decrease of the displacement energy.

  13. Size-dependent distribution and feeding habits of Terebralia palustris in mangrove habitats of Gazi Bay, Kenya

    Science.gov (United States)

    Pape, Ellen; Muthumbi, Agnes; Kamanu, Chomba Peter; Vanreusel, Ann

    2008-03-01

    The gastropod Terebralia palustris often dominates the surface of muddy to sandy substrates of intertidal mudflats and mangrove forests, where they clearly destabilize the sediment. In the present study, it was investigated whether and to what extent the behaviour of juvenile and adult snails differs among habitats (mudflat vs. mangrove stand) in a Sonneratia alba mangal at Gazi Bay, Kenya. For this purpose we: (1) examined their distribution along three land-sea transects; and (2) applied stable isotope analysis to determine the feeding patterns of different-sized snails from the mangrove and mudflat habitats. Additionally, we investigated if these gastropods exert an impact on microphytobenthic (diatom) biomass, and whether this is size-dependent. The latter objective was met by either enclosing or excluding different-sized snails from experimental cages on the intertidal mudflat and the subsequent assessment of a change in pigment concentration of the sediment surface. In agreement with several previous studies conducted in other mangroves and geographical locations, a spatial segregation was demonstrated between juveniles (more common on the mudflat) and adults (more common in the mangrove forest). On the intertidal mudflat juveniles avoided sediment patches characterized by highly saline water in intertidal pools and a high mud content, while adults tended to dwell on substrates covered by a high amount of leaf litter. Stable carbon isotope analysis of the foot tissue of snails sampled from the S. alba stand and the mudflat indicated a transition in food source when a shell length of 51 mm is reached. Considering the δ13C value of juveniles, it seems they might be selecting for microphytobenthos, which might explain their preference for the mudflat. The diet of size classes found in both habitats did not differ significantly, although juveniles inhabiting the mangrove forest were slightly more depleted in 13C compared to those residing on the mudflat

  14. Modeling climate change, urbanization, and fire effects on Pinus palustris ecosystems of the southeastern U.S.

    Science.gov (United States)

    Costanza, Jennifer K; Terando, Adam J; McKerrow, Alexa J; Collazo, Jaime A

    2015-03-15

    Managing ecosystems for resilience and sustainability requires understanding how they will respond to future anthropogenic drivers such as climate change and urbanization. In fire-dependent ecosystems, predicting this response requires a focus on how these drivers will impact fire regimes. Here, we use scenarios of climate change, urbanization and management to simulate the future dynamics of the critically endangered and fire-dependent longleaf pine (Pinus palustris) ecosystem. We investigated how climate change and urbanization will affect the ecosystem, and whether the two conservation goals of a 135% increase in total longleaf area and a doubling of fire-maintained open-canopy habitat can be achieved in the face of these drivers. Our results show that while climatic warming had little effect on the wildfire regime, and thus on longleaf pine dynamics, urban growth led to an 8% reduction in annual wildfire area. The management scenarios we tested increase the ecosystem's total extent by up to 62% and result in expansion of open-canopy longleaf by as much as 216%, meeting one of the two conservation goals for the ecosystem. We find that both conservation goals for this ecosystem, which is climate-resilient but vulnerable to urbanization, are only attainable if a greater focus is placed on restoration of non-longleaf areas as opposed to maintaining existing longleaf stands. Our approach demonstrates the importance of accounting for multiple relevant anthropogenic threats in an ecosystem-specific context in order to facilitate more effective management actions.

  15. Modeling climate change, urbanization, and fire effects on Pinus palustris ecosystems of the southeastern U.S.

    Science.gov (United States)

    Costanza, Jennifer; Terando, Adam; McKerrow, Alexa; Collazo, Jaime

    2015-01-01

    Managing ecosystems for resilience and sustainability requires understanding how they will respond to future anthropogenic drivers such as climate change and urbanization. In fire-dependent ecosystems, predicting this response requires a focus on how these drivers will impact fire regimes. Here, we use scenarios of climate change, urbanization and management to simulate the future dynamics of the critically endangered and fire-dependent longleaf pine (Pinus palustris) ecosystem. We investigated how climate change and urbanization will affect the ecosystem, and whether the two conservation goals of a 135% increase in total longleaf area and a doubling of fire-maintained open-canopy habitat can be achieved in the face of these drivers. Our results show that while climatic warming had little effect on the wildfire regime, and thus on longleaf pine dynamics, urban growth led to an 8% reduction in annual wildfire area. The management scenarios we tested increase the ecosystem's total extent by up to 62% and result in expansion of open-canopy longleaf by as much as 216%, meeting one of the two conservation goals for the ecosystem. We find that both conservation goals for this ecosystem, which is climate-resilient but vulnerable to urbanization, are only attainable if a greater focus is placed on restoration of non-longleaf areas as opposed to maintaining existing longleaf stands. Our approach demonstrates the importance of accounting for multiple relevant anthropogenic threats in an ecosystem-specific context in order to facilitate more effective management actions.

  16. An evaluation of memory accuracy in food hoarding marsh tits Poecile palustris--how accurate are they compared to humans?

    Science.gov (United States)

    Brodin, Anders; Urhan, A Utku

    2013-07-01

    Laboratory studies of scatter hoarding birds have become a model system for spatial memory studies. Considering that such birds are known to have a good spatial memory, recovery success in lab studies seems low. In parids (titmice and chickadees) typically ranging between 25 and 60% if five seeds are cached in 50-128 available caching sites. Since these birds store many thousands of food items in nature in one autumn one might expect that they should easily retrieve five seeds in a laboratory where they know the environment with its caching sites in detail. We designed a laboratory set up to be as similar as possible with previous studies and trained wild caught marsh tits Poecile palustris to store and retrieve in this set up. Our results agree closely with earlier studies, of the first ten looks around 40% were correct when the birds had stored five seeds in 100 available sites both 5 and 24h after storing. The cumulative success curve suggests high success during the first 15 looks where after it declines. Humans performed much better, in the first five looks most subjects were 100% correct. We discuss possible reasons for why the birds were not doing better.

  17. Effects of Restoration Techniques on Soil Carbon and Nitrogen Dynamics in Florida Longleaf Pine (Pinus palustris Sandhill Forests

    Directory of Open Access Journals (Sweden)

    Martin Lavoie

    2014-03-01

    Full Text Available Historic fire suppression and intensive forest management in longleaf pine (Pinus palustris sandhill forests has resulted in hardwood encroachment and degradation of this fire-dependent ecosystem. Active management is now required to restore native community structure and composition, but little is known about the long-term impacts of typical restoration techniques on ecosystem properties. In 1994, the Longleaf Pine Restoration Project (LPRP was established in fire-excluded longleaf pine sandhills of Eglin Air Force Base, Florida, to explore the effects of restoration treatments on plant and animal community composition and soil processes. Experimental treatments applied included three hardwood reduction techniques and delayed burn. Reference sites were concurrently monitored. Fifteen years later, we revisited the LPRP plots to determine whether soil processes showed lasting treatment effects. This study showed that there were no differences in soil C and N between the reference and the fire-suppressed plots prior to the treatments, suggesting that soil C and N were relatively resistant to degradation. This study also showed that the restoration treatments had a significant effect by reducing soil C, but this effect was only short-lived (<3 years. In addition, a MRPP (multi-response permutation procedure analysis showed that only the herbicide treatment was still different from the reference plots 15 years after the initial treatments. Thus, this study suggests that repeated fires (or lack of or hardwood removal treatments have little detectable effect on soil nutrients in these nutrient-poor ecosystems.

  18. Verhaltensweisen und Medien - Änderungen der Habitusformen durch den Gebrauch von neuen Medien in Österreichs Schulen

    Directory of Open Access Journals (Sweden)

    Ursula Dopplinger

    2014-03-01

    Full Text Available Stellt man die Frage, wie sich die Habitusformen jener Personen, die direkt oder indirekt im österreichische Schulwesen involviert sind, durch den Gebrauch von (neuen Medien geändert haben, müssen im Vorfeld einige theoretische und epistemologische Aspekte aufgezeigt werden. Diese bilden die Grundlage, um darstellen zu können, was für eine zielführende Umsetzung eines Bildungsprozesses in Hinblick auf die Mediendidaktik und der daraus resultierenden Medienkompetenz der Schüler/innen in Österreich notwendig ist. Mit anderen Worten wird der Frage nachgegangen, welche Rolle der Mediale Habitus in den sozialen Feldern des Unterrichts spielt? Normen, die dabei zum Tragen kommen und welche Prozesse durch den Einsatz von neuen Medien im Unterricht hervorgerufenen werden, veranschaulicht dieser Artikel.

  19. Differentielle Regulation von Schlüsselgenen der gastralen Säuresekretion durch Gastrin, oxidativen Stress und Helicobacter pylori

    OpenAIRE

    Höcker, Michael

    2002-01-01

    Die transkriptionelle Aktivierung des HDC Gens sowie des Chromogranin A Gens in ECL-Zellen der Magenmucosa repräsentiert einen zentralen Mechanismus der Säureregulation durch Gastrin und scheint ausserdem Bedeutung für die Pathogenese der gastroduodenalen Ulkuskrankheit zu haben. Unsere Untersuchungen identifizieren erstmals die molekularen Mechanismen der Gastrin-abhängigen Regulation beider Gene und definieren die beteiligten Transkriptionsfaktoren, regulatorischen DNA-Elemente und intrazel...

  20. Light-Driven Amino Acid Uptake in Streptococcus cremoris or Clostridium acetobutylicum Membrane Vesicles Fused with Liposomes Containing Bacterial Reaction Centers

    NARCIS (Netherlands)

    Crielaard, Wim; Driessen, Arnold J.M.; Molenaar, Douwe; Hellingwerf, K; Konings, Wilhelmus

    Reaction centers of the phototrophic bacterium Rhodopseudomonas palustris were introduced as proton motive force-generating systems in membrane vesicles of two anaerobic bacteria. Liposomes containing reaction center-light-harvesting complex I pigment protein complexes were fused with membrane

  1. Mechatronics in the car: success through efficient methodological development; Mechatronik im Kraftfahrzeug: Erfolg durch effiziente Entwicklungsmethodik

    Energy Technology Data Exchange (ETDEWEB)

    Dieterle, W.; Dilger, E. [Bosch (R.) GmbH, Gerlingen (Germany). Zentralbereich Forschung und Vorausentwicklung; Buerger, K.G. [Bosch (R.) GmbH (Germany)

    2001-04-01

    The ever increasing requirements with respect to fuel consumption, emissions as well as safety and comfort necessitate ever increasing complex functionalities in the car. They are realized, as a rule, by a combination of mechanics, hydraulics, electrics/electronics and, more and more, software. In the past, the development of the various electric/electronic, mechanical or hydraulic components tended to happen at separate levels, nowadays the focus is more on a comprehensive approach to the overall system. In this context, the integrated development of 'mechatronic' components, including the respective software, leads to a targeted solution. (orig.) [German] Staendig steigende Anforderungen hinsichtlich Kraftstoffverbrauch, Emissionen sowie Sicherheit und Komfort bedingen immer komplexere Funktionalitaeten im Kraftfahrzeug. Sie werden in der Regel durch eine Kombination von Mechanik, Hydraulik, Elektrik/Elektronik und zunehmend auch Software realisiert. Nachdem in der Vergangenheit die Entwicklung meist noch auf getrennten Ebenen verlief, steht heute ein uebergreifender, das Gesamtsystem umfassender Ansatz im Vordergrund. Dieser Beitrag der Robert Bosch GmbH berichtet ueber aktuelle Mechatronik-Entwicklungen. Dabei sorgt die integrierte Entwicklung der 'mechatronischen' Komponenten unter Einbezug der zugehoerigen Software fuer eine zielgerichtete Loesung. (orig.)

  2. Processing of maize plants by rotary kiln pyrolysis; Veredlung von Maispflanzen durch Pyrolyse im Drehrohrreaktor

    Energy Technology Data Exchange (ETDEWEB)

    Klose, W.; Wiest, W. [Kassel Univ. (Gesamthochschule) (Germany). Inst. fuer Thermische Energietechnik

    1996-12-31

    The fuel quality of maize plants is to be characterized by short, elementary and thermonalysis. The plants will be pyrolyzed in order to facilitate transport and storage. The formal kinetic parameters of three parallel reactions describing solid matter decomposition are defined by means of TG-DSC, and the reaction enthalpy is measured. Pyrolysis experiments in a rotary kiln converter in the kg range show a strong dependence of the product spectrum on process temperature. In particular, the pyrolysis gas yield increases with temperature at the expense of pyrolysis oil and water. (orig) [Deutsch] Zur energetischen Nutzung von Kulturpflanzen wird die brennstofftechnische Charakterisierung von Maispflanzen durch Kurz-, Elementar- und Thermoanalyse durchgefuehrt. Zur Reduzierung des Aufwands fuer Transport und Lagerung sollen die Pflanzen pyrolysiert weren. Mit Hilfe der TG-DSC werden formalkinetische Parameter von drei Parallelreaktionen zur Beschreibung der Feststoffzersetzung bestimmt und die Reaktionsenthalpie gemessen. Pyrolyseversuche in einem Drehrohrreaktor im Kilogramm-Massstab ergeben eine starke Abhaengigkeit des Produktspektrums von der Prozesstemperatur. Insbesondere steigt die Pyrolysegasausbeute auf Kosten der Bildung von Pyrolyseoel und Wasser mit der Temperatur stark an. (orig)

  3. Medizintechnik: Neue Möglichkeiten zur Beurteilung menschlicher Eizellen durch das Polarisationsmikroskopische System OCTAX ICSI GUARDTM

    Directory of Open Access Journals (Sweden)

    2006-01-01

    Full Text Available Polarisationsmikroskopie dient der nicht-invasiven Visualisierung von Strukturen, die in der herkömmlichen Lichtmikroskopie keinen oder zu wenig Kontrast aufweisen. Dabei nutzt man die sogenannten doppelbrechenden Eigenschaften von Objekten mit molekular hoch geordneten Strukturen, wie sie z. B. in Form der Mikrotubuli vorliegen.br bKomponenten von OCTAX ICSI Guard™:/bbr Der vorliegende Bericht befaßt sich mit OCTAX ICSI Guard™, einem polarisationsmikroskopischen System, das speziell für die Anforderungen in der Reproduktionsmedizin entwickelt wurde. Mit wenigen optischen Zusatzkomponenten können moderne Inversmikroskope aufgerüstet werden, um polarisationsmikroskopische Bilder von Eizellen zu erhalten. Die optischen Komponenten von ICSI Guard™ beschränken sich auf einen Grünfilter, einen zirkularen Polarisator und einen einstellbaren Flüssigkristall-Analyseschieber, mit dem das zirkular polarisierte Licht gefiltert wird. Die Bilder werden von einer hochauflösenden USB2-Kamera aufgenommen und durch die ICSI Guard™-Bildverarbeitungssoftware ausgewertet und visualisiert. Dies ermöglicht die Livedarstellung doppellichtbrechender Zellstrukturen unabhängig von ihrer Orientierung. Auf dem Bildschirm wird das polarisationsmikroskopische Bild (orange dem Hellfeldbild der Zelle (grün überlagert.br bPolarisationsmikroskopie an menschlichen Eizellen:/bbr In Eizellen haben meiotische Spindeln sowie die Zona pellucida doppelbrechende Eigenschaften. Die Spindel ist verantwortlich für die fehlerfreie Verteilung der Chromosomen bzw. Chromatiden während der meiotischen Reifeteilungen. Sie durchläuft während der verschiedenen Phasen der Meiose dynamische Veränderungen, die auf molekularer Ebene im Auf-, Um-, und Abbau von Mikrotubuli des Spindelapparates begründet sind. Unter guten Laborbedingungen können mit Hilfe der Polarisationsmikroskopie in mindestens 80 % aller Eizellen Spindeln nachgewiesen werden [1]. Die Zona pellucida ist eine

  4. Kocurin, the True Structure of PM181104, an Anti-Methicillin-Resistant Staphylococcus aureus (MRSA) Thiazolyl Peptide from the Marine-Derived Bacterium Kocuria palustris

    Science.gov (United States)

    Martín, Jesús; Sousa, Thiciana da S.; Crespo, Gloria; Palomo, Sara; González, Ignacio; Tormo, José R.; de la Cruz, Mercedes; Anderson, Matthew; Hill, Russell T.; Vicente, Francisca; Genilloud, Olga; Reyes, Fernando

    2013-01-01

    A new thiazolyl peptide, kocurin (1), was isolated from culture broths of a marine-derived Kocuria palustris. Its structural elucidation was accomplished using a combination of spectroscopic and chemical methods, including HRMS, extensive 1D and 2D NMR analysis, MS/MS fragmentation, and chemical degradation and Marfey’s analysis of the resulting amino acid residues. The structure herein reported corrects that previously assigned to PM181104 (3). Kocurin displayed activity against methicillin-resistant Staphylococcus aureus (MRSA), with MIC values in the submicromolar range. PMID:23380989

  5. Kocurin, the True Structure of PM181104, an Anti-Methicillin-Resistant Staphylococcus aureus (MRSA Thiazolyl Peptide from the Marine-Derived Bacterium Kocuria palustris

    Directory of Open Access Journals (Sweden)

    Fernando Reyes

    2013-02-01

    Full Text Available A new thiazolyl peptide, kocurin (1, was isolated from culture broths of a marine-derived Kocuria palustris. Its structural elucidation was accomplished using a combination of spectroscopic and chemical methods, including HRMS, extensive 1D and 2D NMR analysis, MS/MS fragmentation, and chemical degradation and Marfey’s analysis of the resulting amino acid residues. The structure herein reported corrects that previously assigned to PM181104 (3. Kocurin displayed activity against methicillin-resistant Staphylococcus aureus (MRSA, with MIC values in the submicromolar range.

  6. Continuous high-temperature recycling of waste by the Thermoselect process; Unterbrechungsloses Hochtemperaturrecycling - Abfallveredelung durch Thermoselect

    Energy Technology Data Exchange (ETDEWEB)

    Stahlberg, R. [Thermoselect Engineering S.r.l., Verbania (Italy)

    1998-09-01

    Based on the experience with the Thermoselect plant at Fondotoce, Italy (100 Mg/d), which was commissioned in 1992, a 225,000 Mg/a plant with three units (720 Mg/d) is being constructed at Karlsruhe, Germany. The plant is scheduled for commissioning in 1998. The contribution presents the product balance, the approved emission levels, and the energy uses envisaged for the Karlsruhe project. Final product quality data and energy yields illustrate the differences from conventional thermal processes. An ecobalance comparing the Thermoselect process with grate furnaces shows that water, soil and air pollution is much reduced by the new Termoselect process. Apart from high product quality, the plant also has the advantage of reduced dumping cost and no long-term ecological hazards. (orig./SR) [Deutsch] Auf Basis der grosstechnischen Erfahrung mit einer seit 1992 in Fondotoce (Italien) betriebenen THERMOSELECT-Anlage (Kapazitaet 100 Mg/d), Restmuell umweltgerecht zu behandeln, wird 1998 der Aufbau einer 225.000 Mg/a-Anlage mit 3 Linien (720 Mg/d) in Karlsruhe (Deutschland) abgeschlossen. Die Produktebilanz und die genehmigten Emissionswerte sind in dem Artikel belegt. Gezeigt wird die fuer das Projekt Karlsruhe vorgesehene Energienutzung. Die Endprodduktqualitaeten und die Energieausbeute verdeutlichen die Unterschiede zu bisherigen konventionellen thermischen Verfahren. Eine erarbeitete Oekobilanz mit einem Vergleich von THERMOSELECT und Rostfeuerung zeigt die deutlich geringe Belastung der Umweltkompartimente Wasser, Boden und Luft durch das neue THERMOSELECT-Verfahren. Neben hohen Produktqualitaeten wird - verglichen mit traditionellen Techniken - ein deutlich verringerter Entsorgungspreis erreicht, wobei gleichzeitig oekologische Belastungen auszuschliessen sind. (orig./SR)

  7. Ionenquelle mit Plasma-Anregung durch Mikrowellen bei Elektronen-Zyklotron-Resonanz

    Science.gov (United States)

    Hammer, K.; Weissmantel, C.

    Mit der Mikrowellen-Entladung bei Zyklotron-Resonanz ist eine effektive Plasma-Anregung möglich; es lassen sich Ionisationsgrade um 0,1 in Edel- und Reaktivgasen erreichen. Die Nutzung für Ionenquellen bietet gegenüber der unselbständigen Niederdruck-Bogenentladung eine Reihe von Vorteilen, insbesondere durch den Wegfall der Glühkatode. Es wird eine Breitstrahl-Ionenquelle beschrieben, die bei einer Extraktionsspannung von 1 kV Ionenströme bis zu 150 mA liefert. Die Ionenstromdichte läßt sich über einen Strahldurchmesser von 150 mm weitgehend konstant halten. Damit erfüllt die Mikrowellen-Ionenquelle Forderungen, wie sie beim Einsatz von Ionenstrahlverfahren in der Mikroelektronik gestellt werden.Translated AbstractIon Source with Plasma Generation by Microwaves in Electron Cyclotron ResonanceThe microwave discharge operated at cyclotron resonance allows to achieve an effective plasma generation; degrees of ionization of about 0,1 are feasible. The utilization in ion sources offers compared with non-selfsustained are discharges several advantages, in particular because the hot cathode is emitted. A broad-beam ion source is described which yields ion currents up to 150 mA at an extraction voltage of 1 kV. The ion current density could be maintained nearly constant over a beam diameter of 150 mm. Hence, the microwave ion source is shown to match the requirements for applications of ion beam processes in microelectronics.

  8. Dust explosion hazards. Comments on a topical debate; Gefahren durch Staubexplosionen. Anmerkungen zu einer aktuellen Debatte

    Energy Technology Data Exchange (ETDEWEB)

    Uth, H.J. [Umweltbundesamt, Berlin (Germany). Fachgebiet Anlagensicherheit und Stoerfallvorsorge; Wietfeldt, P.

    1999-06-01

    The Federal Republic of Germany as a Member State of the EU is about to translate the Seveso II EU Directive into national law. The directive shall be incorporated into the German emergency management ordinance (StoerfallV) currently under review, and then will be a part of the German emission control law. A draft ordinance for amendment prepared by the German BMU as the responsible ministry was distributed to the parties involved for discussion and comment in January 1999, and the resulting ministry draft ordinance recently has been presented for debate. Especially representatives of the branches of industry concerned argued that the existing provisions in the German StoerfallV, relating to protection against hazards emanating from dust/air mixtures, are made redundant by the incorporation of the Seveso II EU Directive and should be deleted. The authors examine whether this opinion is right in the context of intention of the legislator as well as facts. (orig./CB) [Deutsch] Die Bundesrepublik Deutschland hat die Anforderungen der Seveso-II-Richtlinie in nationales Recht umzusetzen. Dies soll im Rahmen des Immissionschutzrechts durch eine grundlegende Novellierung der Stoerfall-Verordnung geschehen. Hierzu hat das Bundesumweltministerium einen Entwurf erstellt und die nach Paragraph 7 BImSchG erforderliche Anhoerung der beteiligten Kreise sowie der Bundeslaender Mitte Januar 1999 durchgefuehrt. Inzwischen liegt ein ueberarbeiteter Referentenentwurf vor. Bei den Eroerterungen des Entwurfs wurde insbesondere von den Vertretern der betroffenen Industrien vorgetragen, dass die Regelung zum Schutz vor explosionsfaehigen Staub/Luft-Gemischen im Rahmen der Stoerfall-Verordnung ueberfluessig seien und bei der Umsetzung der Seveso-II-Richtlinie in nationales Recht nicht weiter uebernommen werden sollten. (orig.)

  9. Purification of L-glutamate-dependent citrate lyase from Clostridium sphenoides and electron microscopic analysis of citrate lyase isolated from Rhodopseudomonas gelatinosa, Streptococcus diacetilactis and C. sphenoides.

    Science.gov (United States)

    Antranikian, G; Klinner, C; Kümmel, A; Schwanitz, D; Zimmermann, T; Mayer, F; Gottschalk, G

    1982-08-01

    Citrate lyase from Clostridium sphenoides was purified 72-fold with a yield of 11%. In contrast to citrate lyase from other sources the activity of this enzyme was strictly dependent on the presence of L-glutamate. The purified enzyme was only stable in the presence of 150 mM L-glutamate or 7 mM L-glutamate plus glycerol, sucrose or bovine serum albumin. Changes of the L-glutamate pool and of enzyme activity in growing cells of C. sphenoides indicated that citrate lyase activity in this organism was regulated by the intracellular L-glutamate concentration. Citrate lyase isolated from C. sphenoides, Rhodopseudomonas gelatinosa and Streptococcus diacetilactis was investigated by electron microscopy using the negative staining technique. Three different projections of enzyme molecules were observed: 'star' form, 'ring' form and 'triangle' form. In samples from R. gelatinosa and S. diacetilactis, star and ring forms occurred in a ratio of about 1:9. Using the enzyme from S. diacetilactis it was demonstrated that this ratio could be altered in favour of the star form by the addition of citrate or tricarballylate. The triangle form was observed in less than 1% of all evaluated molecules and may represent a transition form. In lyase samples from C. sphenoides there existed a correlation between enzyme activity and the proportion of stars and rings at varying concentrations of L-glutamate.

  10. A Tribal Story Written in Silica: Using Phytoliths to Research the Effects of Mining on Past Wild Rice (Zizania palustris) Abundance in Sandy Lake, Minnesota

    Science.gov (United States)

    Clarke, I. R.; Jones, M. A.; Yost, C. L.; Drake, C.; Ladwig, J. L.; Myrbo, A.; Howes, T.

    2014-12-01

    Wild rice (Zizania palustris, manoomin) is an emergent aquatic plant that grows annually in the northern Great Lakes region of North America. This region is also rich in iron ore deposits and correspondingly has an extensive history of mining activities. Wild rice no longer grows in some areas where it was previously abundant. Sandy Lake, located in St. Louis County on federally protected lands that are ceded territory of the Fond du Lac Band of Lake Superior Chippewa in Minnesota and downstream of the nearby U.S. Steel Minntac mine, was selected as a test site. This lake has a history of ricing activities by the Ojibwe (Chippewa) People, for whom manoomin has cultural importance. Lake cores were taken on June 17, 2014 by LacCore and FDLRM staff and samples were obtained. This project used phytolith analysis to answer the question of past wild rice presence and abundance in Sandy Lake. Phytoliths are microscopic opal silica deposits produced in some plants. Zizania palustris produces phytolith morphotypes that are unequivocally diagnostic of this species in this region. Microscopic slides were prepared and analyzed for wild rice phytoliths. Concentration values ranged from 25 to 4379 phytoliths per cm3/year, and wild rice accumulation figures ranged from 7 to 789 phytoliths/cm2/year, the maximum values of which occurred in the 1920s and generally declined to the current lowest levels observed. Mining has likely impacted wild rice populations by causing increased sulfate levels and possibly contributing to higher lake levels.

  11. 光合细菌与产气肠杆菌协同产氢特性分析%Characteristic Analysis of Cooperation Hydrogen Production Using Rhodopseudomonas sp.DT and Enterobacter Aerogenes

    Institute of Scientific and Technical Information of China (English)

    张晓蓉; 龚双娇; 廖惠敏; 杨冬梅; 陈义光

    2009-01-01

    Cooperation hydrogen production was carried out using Rhodopseudomonas sp. DT and Entero-bacter aerogenes. The effects of the initial ratio of Rhodopseudomonas sp. DT and E. aerogenes, culture temperature, and carbon source on the cooperation hydrogen production were investigated. The results suggested that cooperation hydrogen production rate was highly affected by the initial ratio of Rhodopseudomonas sp. DT and E. aerogenes. The mixed bacteria of Rhodopseudomonas sp. DT and E. aerogenes with 1:1 initial ratio benefited to the cooperation hydrogen production, which led the hydrogen production rate and duration of gas production to 3.1 mol H_2/mol glucose and 81 h, respectively. The pH dynamics analysis of culture medium further discovered that the pH of the mixed bacteria with 1:1 initial ratio changed from 6 to 7 smaller than other conditions, which was probably fitted to produce hydrogen. Furthermore, the mixed bacteria with 1:1 initial ratio had the higher hydrogen production efficiency at temperatures of 28? and 37? than at 20?, and without any hydrogen production at temperature of 50?. The carbon sources of glucose, succinate acid, malic acid could be used to produce hydrogen by the mixed bacteria. Even the solu-ble starch, unused by Rhodopseudomonas sp. DT, was also decomposed by the mixed bacteria to produce hydrogen with the conversion efficiency of 8.22%. The glucose was the optimal carbon resource, and the conversion efficiency could reach to 36.11%. The results, further, implied that the cooperation hydrogen production could enlarge the use of the carbon sources.%对光合细菌(Rhodopseudomonas sp.DT)与产气肠杆菌(Enterobacter aerogenes)进行了发酵产氢试验,考察了不同起始接种比例、培养温度及碳源条件下混合菌协同产氢特性.结果表明:光合细菌与产气肠杆菌初始接种比例对协同产氢影响较大,初始接种比例为1:1最有利于协同产氢,产氢效率和产氢周期达到了3.1 mol H_2/mol

  12. Action of sulfurous acid on pollen. [Hepatica triloba; Helleborus orientalis; Vinca minor; Viola tricolor; Primula officinalis; Lilium candidum; Petunia; Pisum; Helleborus viridus; Galanthus nivealis; Vinca major; Convallaria maialis; Narcissus poeticus; Caltha palustris; Cystisus laburnum; Orchis maculata; Bilbergia; Eranthus; Crocus

    Energy Technology Data Exchange (ETDEWEB)

    Sabachnikoff, V.

    1912-01-23

    The following ornamental plants: Hepatica triloba, Helleborus orientalis, Vinca minor, Viola tricolor, Primula officinalis, Lilium candidum, Petunia, Pisum, Helleborus viridus, Galanthus nivealis, Vinca major, Convallaria maialis, Narcissus poeticus, Caltha palustris, Cystisus laburnum, Orchis maculata, Bilbergia, Eranthus, and Crocus were tested for seed production. Exposure to sulfuric acid ranged from three to forty-eight hours. Responses were noted for varying concentrations.

  13. Pollution and pollution tolerance in the case of polycyclic aromatic hydrocarbons (PAH); Belastung durch Polyzyklische aromatische Kohlenwasserstoffe (PAK)

    Energy Technology Data Exchange (ETDEWEB)

    Renger, M.; Mekiffer, B. [Technische Univ. Berlin (Germany). Inst. fuer Oekologie-Bodenkunde

    1997-12-31

    The purpose of the present follow-up project was to examine the contamination with polycyclic aromatic hydrocarbons (PAH) of different anthropogenic urban soils including clay soils containing demolition waste, household waste, ash, and residues from a coking plant. A further task was to analyse, or infer from other part-projects, standard soil parameters such as organic carbon content, pH, and anion levels in order to clarify any relationships between PAH contamination and the more easily determinable soil characteristics. Furthermore, the sorption behaviour for PAH of selected anthropogenic urban soils was to be characterised by means of batch experiments. [Deutsch] Im Rahmen des Anschlussvorhabens sollte die Kontamination von anthropogenen Stadtboeden- darunter Truemmerschutt-, Hausmuell-, Asche- sowie Kokereilehmboden- durch polyzyklische aromatische Kohlenwasserstoffe (PAK) untersucht werden. Zusaetzlich sollten die bodenkundlichen Standardparameter Corg, pH-Wert, Anionengehalte und KAKpot analysiert bzw. von den anderen Teilvorhaben uebernommen werden, um Zusammenhaenge zwischen der PAK-Kontamination und relativ leicht zu bestimmenden bodenkundlichen Kennwerten klaeren zu koennen. Das Sorptionsverhalten ausgewaehlter anthropogener Stadtboeden fuer PAK sollte durch Batchversuche charakterisiert werden. (orig./SR)

  14. Microbiological studies in enhanced sewage sludge degradation through cell membrane break-up; Mikrobiologische Untersuchungen zum verbesserten Klaerschlammabbau durch Zellaufschluss. Mechanische und thermische Behandlung von Schlaemmen

    Energy Technology Data Exchange (ETDEWEB)

    Battenberg, S.; Naeveke, R. [Gesellschaft fuer Biotechnologische Forschung mbH, Braunschweig (Germany). Bereich Mikrobiologie

    1999-07-01

    Mechanical disintegration breaks up the flake structure of excess sludge and solubilizes organic constituents. Bacterial cells are rendered soluble. Thermal conditioning of excess sludge, too, can release sludge constituents. This makes for enhanced and accelerated hydrolysis of polymeric sludge constituents in subsequent anaerobic processing. The yield of useful biogas is increased. (orig.) [German] Durch die mechanische Desintegration von Ueberschussschlamm wird die Flockenstruktur zerschlagen und organische Inhaltsstoffe werden in Loesung gebracht. Bakterienzellen werden aufgeschlossen. Auch durch eine thermische Behandlung von Ueberschussschlamm koennen Schlamminhaltsstoffe freigesetzt werden. Dadurch wird bei einer anschliessenden anaeroben Behandlung die Hydrolyse der polymeren Schlamminhaltsstoffe gefoerdert und beschleunigt. Die Ausbeute an verwertbarem Biogas wird gesteigert. (orig.)

  15. 沼泽红假单胞菌对微囊藻毒素的降解%Degradation of microcystin by Rhodopseudomonas palustras.

    Institute of Scientific and Technical Information of China (English)

    李保珍; 冯佳; 谢树莲

    2012-01-01

    以铜绿微囊藻为材料,通过固相萃取-高效液相色谱方法( SPE-HPLC),研究了沼泽红假单胞菌对微囊藻毒素MC-LR的降解作用.结果表明:沼泽红假单胞菌在厌氧、光照强度2000 lx、35℃、pH 7.0、乙酸钠为碳源、菌液初始浓度OD680为0.325和初始MC-LR为3mg·L-1时,6d降解率为36.5%,12d达到最高,降解率为78.7%.此降解条件和蓝藻水华爆发的环境条件基本一致,因此该菌株在水华爆发季节消除水中的微囊藻毒素方面具有应用潜力.%Taking Microcystis aeruginosa as test material, SPE-HPLC was adopted to study the degradation of microcystin-LR by Rhodopseudomonas palustras. Under the conditions of anaero bic, light intensity 20001×35 ℃ , pH 7.0, carbon source sodium acetate, and initial concentration OD680 of the bacilli being 0. 325 , R. Palustras could degrade 36. 5% and 78. 7% of the microcystin-LR with an initial concentration 3 mg ? L-1 in 6 d and 12 d, respectively. These deg radation conditions were basically the same environmental conditions of algal blooms, and hence, R. Palustras could have practical value for removing microcystin-LR from the water body during algal blooms.

  16. Does the reconstitution of RC-LH1 complexes from Rhodopseudomonas acidophila strain 10050 into a phospholipid bilayer yield the optimum environment for optical spectroscopy?

    Science.gov (United States)

    Böhm, Paul S; Kunz, Ralf; Southall, June; Cogdell, Richard J; Köhler, Jürgen

    2013-12-01

    We have investigated reaction-center light-harvesting 1 (RC-LH1) complexes from Rhodopseudomonas (Rps.) acidophila in detergent buffer solution and reconstituted into a phospholipid bilayer and compared the results with the outcome of an earlier study conducted on RC-LH1 immobilized in polyvinyl alcohol (PVA). The aim of this study was to test whether the immobilization of the complexes in a PVA matrix might lead to a deterioration of the proteins and thereby limit the accessible information that can be obtained from optical spectroscopy. It has been found that the complexes dissolved in a detergent buffer solution are subject to fast spectral dynamics preventing any meaningful application of single-molecule spectroscopy. In contrast, for the bilayer samples it is revealed that the reconstitution process results in a significantly larger fraction of broken complexes with respect to the preparation of the complexes in a PVA film. Moreover, we find that for the intact complexes the statistics of the key spectral features, such as the spectral separations of the bands and the mutual orientation of their transition-dipole moments, show no variation dependent on using either a bilayer or PVA as a matrix. Given the additional effort involved in the reconstitution process, the lower amount of intact RC-LH1 complexes and, concerning the decisive spectral details, the identical results with respect to embedding the complexes in a PVA matrix, we come to the conclusion that the immobilization of these proteins in a PVA matrix is a good choice for conducting low-temperature experiments on individual light-harvesting complexes.

  17. Femtosecond and hole-burning studies of B800`s excitation energy relaxation dynamics in the LH2 antenna complex of Rhodopseudomonas acidophila (strain 10050)

    Energy Technology Data Exchange (ETDEWEB)

    Wu, H.M.; Savikhin, S.; Reddy, N.R.S.; Jankowiak, R.; Struve, W.S.; Small, G.J. [Ames Lab., IA (United States)]|[Iowa State Univ., Ames, IA (United States); Cogdell, R.J. [Univ. of Glasgow (United Kingdom)

    1996-07-18

    One- and two-color pump/probe femtosecond and hole-burning data are reported for the isolated B800-850 (LH2) antenna complex of Rhodopseudomonas acidophila (strain 10050). The two-color profiles are interpretable in terms of essentially monophasic B800{yields}B850 energy transfer with kinetics ranging from 1.6 to 1.1 ps between 19 and 130 K for excitation at or to the red of the B800 absorption maximum. The B800 zero-phonon hole profiles obtained at 4.2 K with burn frequencies located near or to the red of this maximum yielded a transfer time of 1.8 ps. B800 hole-burning data (4.2 K) are also reported for chromatophores at ambient pressure and pressures of 270 and 375 MPa. At ambient pressure the B800-B850 energy gap is 950 cm{sup -1}, while at 270 and 375 MPa it is close to 1000 and 1050 cm{sup -1}, respectively. However, no dependence of the B800{yields}B850 transfer time on pressure was observed. The resilience of the transfer rate to pressure-induced changes in the energy gap and the weak temperature dependence of the rate are consistent with the model that has the spectral overlap (of Foerster theory) provided by the B800 fluorescence origin band and weak vibronic absorption bands of B850. However, both the time domain and hole-burning data establish that there is an additional relaxation channel for B800, which is observed when excitation is located to the blue of the B800 absorption maximum. 40 refs., 11 figs., 6 tabs.

  18. The Didactic Model "LdL" (Lernen Durch Lehren) as a Way of Preparing Students for Communication in a Knowledge Society

    Science.gov (United States)

    Grzega, Joachim; Schoner, Marion

    2008-01-01

    Based on studies in learning psychology, biology and education, the original technique "Lernen durch Lehren" ("LdL)" (German for "learning by teaching") has been elaborated into a meta-model ("Meta") "LdL" that aims at giving students a platform to acquire the competencies considered necessary for knowledge societies. Ninety-seven former students…

  19. Charakterisierung der molekularen Determinanten zur hochaffinen Bindung der kompatiblen Soluten Glycin Betain, Prolin Betain, Ectoin und Hydroxyectoin durch Substratbindeproteine von bakteriellen ABC-Transportern

    OpenAIRE

    Sohn-Bösser, Linda

    2006-01-01

    Die Akkumulation von kompatiblen Soluten ist ein weit verbreiteter Schutzmechanismus gegen variierende Umweltbedingungen und wird in vielen Spezies der Bacteria und Archaea verwendet. Das einige kompatible Solute nicht nur osmoprotektive Wirkung haben, sondern generell Protein-stabilisierende Substanzen sind, ist durch in vitro Experimente belegt. Der Protein-stabilisierende Effekt wird in dem „preferential exclusion model“ beschr...

  20. Contrast generation in the nuclear-spin tomography by pulsed ultrasound; Kontrasterzeugung in der Kernspintomographie durch gepulsten Ultraschall

    Energy Technology Data Exchange (ETDEWEB)

    Oehms, Ole Benjamin

    2009-07-10

    In the framework of this thesis a combined method of ultrasound and nuclear-spin tomography is presented. Via ultrasound pulses by the sound-radiation force in liquids and tissue phantoms motions are generated, which depend on ther viscoelastic properties. This motions are made visible by a motion-sensitive tomograph sequence in the phase image of the tomograph in form of a phase change. The first measurements on simple phantoms and liquids are presented. [German] Im Rahmen dieser Arbeit wird eine kombinierte Methode aus Ultraschall und Kernspintomographie vorgestellt. Ueber Ultraschallpulse werden durch die Schallstrahlungskraft in Fluessigkeiten und Gewebephantomen Bewegungen erzeugt, die von den viskoelastischen Eigenschaften abhaengen. Diese Bewegungen werden mit einer bewegungssensitiven Tomographensequenz im Phasenbild des Tomographen in Form einer Phasenaenderung sichtbar gemacht. Die ersten Messungen an einfachen Phantomen und Fluessigkeiten werden praesentiert. (orig.)

  1. Verbesserung der Lebensqualität bei Kinderwunsch-Behandlung durch den Gebrauch patientenfreundlicher Follitropin-Fertigpens

    Directory of Open Access Journals (Sweden)

    Bitzer J

    2011-01-01

    Full Text Available Zur Behandlung des unerfüllten Kinderwunsches unterziehen sich viele Frauen einer assistierten Reproduktionstherapie mit ovarieller Stimulation. Dazu erfolgen tägliche subkutane Injektionen mit Gonadotropinen, welche die Patientinnen eigenständig durchführen können. Häufig haben Patientinnen dabei Angst vor Spritzen und injektionsbedingten Schmerzen. Die vorliegende Beobachtungsstudie zum Einfluss der Anwendung des Follitropin alfa-Fertigpens (Gonal-f®-Pen auf die Lebensqualität der Patientinnen zeigt die Ergebnisse einer Befragung von 170 Frauen zu Angst und Zufriedenheit mit diesem Applikationssystem. Die Erfahrungen wurden vor und nach den Behandlungszyklen auf numerischen Analogskalen quantifiziert und nachfolgend verglichen. Die Studie ergab, dass durch die Verwendung des Gonal-f®-Pens die Angst vor der Injektion und vor Schmerzen signifikant abnahm. Zudem waren die Patientinnen mit dem Applikationssystem äußerst zufrieden.

  2. Cytogenetic characterization and fluorescence in situ hybridization of (GATA)10 repeats on established primary cell cultures from Indian water snake (Natrix piscator) and Indian mugger (Crocodylus palustris) embryos.

    Science.gov (United States)

    Rao, L; Turlapati, R; Patel, M; Panda, B; Tosh, D; Mangalipalli, S; Tiwari, A; Orunganti, V P; Rose, D; Anand, A; Kulashekaran, M K; Priya, S R; Mishra, R K; Majumdar, K; Aggarwal, R K; Singh, L

    2009-01-01

    Sex determination among reptiles has continued to draw the attention of geneticists and the mechanisms involved have been extensively studied and documented in the past 3 decades. The setting up of primary cell lines of reptilian tissues is an important tool in the present study which is a unique aspect not applied in earlier studies. Establishing the cell lines from various species of reptiles would help in our understanding of the mechanisms of evolution and differentiation of sex chromosomes. Therefore, in the present study, we have established for the first time primary cell cultures from Indian water snake (Natrix piscator) and Indian mugger (Crocodylus palustris) embryos. In the preliminary growth stage, 2 types of cells, fibroblast- and epithelial-like, were found to be attached and proliferating in vitro. These fibroblast-like cell cultures were later overtaken by epithelial cells. The cell lines were grown in minimal essential medium supplemented with fetal bovine serum and subcultured for a period of 8-10 months. The morphology of cell types was kept under constant observation microscopically. Interestingly, at a subsequent passage of the cells sporadically scattered neuronal-like and beating cells were observed. The suitable temperature for growth of these cell cultures was 28-30 degrees C. Chromosome analysis was performed from the actively proliferating cells, which revealed 5 pairs of macrochromosomes and 15 pairs of microchromosomes in Natrix piscator, and 15 pairs of only macrochromosomes in Crocodylus palustris. (GATA)(n) repeats are well known to be associated with sex chromosomes. Fluorescence in situ hybridization performed with (GATA)(10) repeats delineated the W chromosome in the cells of Natrix piscator which has so far not been reported. This cell culture method has presently only been applied to water snakes and crocodile embryos in the current study, but it will be employed in other reptilian species and could go a long way to being a

  3. Konstruktion, Aufbau und Erprobung einer Einzelionen-Bestrahlungsapparatur und Untersuchung der Inaktivierung von Sporen von bacillus subtilis durch einzelne sehr schwere Ionen (Z $>$ 27) in Abhängigkeit vom Stossparameter

    CERN Document Server

    Weisbrod, U

    1988-01-01

    Konstruktion, Aufbau und Erprobung einer Einzelionen-Bestrahlungsapparatur und Untersuchung der Inaktivierung von Sporen von bacillus subtilis durch einzelne sehr schwere Ionen (Z $>$ 27) in Abhängigkeit vom Stossparameter

  4. Stufenweise Ionisation positiv geladener Kohlenstoffund Stickstoffionen durch Elektronenstoss ($C^{2+} \\to C^{3+} \\to C^{4+} \\to C^{5+} \\to C^{6+}$ und $N^{3+} \\to N^{4+} \\to N^{5+} \\to N^{6+} \\to N^{7+}$)

    CERN Document Server

    Donets, E D; Donets, E D no 1; Ilyushchenko, V I no 1

    1975-01-01

    Stufenweise Ionisation positiv geladener Kohlenstoffund Stickstoffionen durch Elektronenstoss ($C^{2+} \\to C^{3+} \\to C^{4+} \\to C^{5+} \\to C^{6+}$ und $N^{3+} \\to N^{4+} \\to N^{5+} \\to N^{6+} \\to N^{7+}$)

  5. Quantitative fractography under light microscopy: A digital image processing approach; Quantitative Fraktographie mittels Lichtmikroskopie: Naeherung durch digitale Bildverarbeitung

    Energy Technology Data Exchange (ETDEWEB)

    Horovistiz, A.L.; Ribeiro, L.M.F.; Campos, K.A.; Jesuino, G.A.; Guimaraes, V.A.; Hein, L.R.O. [UNESP, Guaratingueta, SP (Brazil)

    2003-02-01

    This work is an example of the improvement on quantitative fractography by means of digital image processing and light microscopy. Two techniques are presented to investigate the quantitative fracture behavior of Ti-4Al-4V heat-treated alloy specimens, under Charpy impact testing. The first technique is the Minkowski method for fractal dimension measurement from surface profiles, revealing the multifractal character of Ti-4Al-4V fracture. It was not observed a clear positive correlation of fractal values against Charpy energies for Ti-4Al-4V alloy specimens, due to their ductility, microstructural heterogeneities and the dynamic loading characteristics at region near the V-notch. The second technique provides an entire elevation map of fracture surface by extracting in-focus regions for each picture from a stack of images acquired at successive focus positions, then computing the surface roughness. Extended-focus reconstruction has been used to explain the behavior along fracture surface. Since these techniques are based on light microscopy, their inherent low cost is very interesting for failure investigations. (orig.) [German] Diese Arbeit ist ein Beispiel fuer die Verbesserung der quantitativen Fraktographie mittels digitaler Bildverarbeitung und Lichtmikroskopie. Zur Untersuchung des quantitativen Bruchverhaltens von waermebehandelten Ti-4Al-4V-Proben im Charpy-Kerbschlagversuch werden zwei Techniken vorgestellt. Die erste Technik ist die Minkowski-Methode zur Messung der fraktalen Dimensionen aus Oberflaechenprofilen, welche den multifraktalen Charakter des Bruches von Ti-4Al-4V ergibt. Es wurde keine eindeutige positive Korrelation zwischen den fraktalen Werten und den Charpyenergien der Ti-4Al-4V-Proben aufgrund deren Duktilitaet, Gefuegeheterogenitaeten und dynamischen Belastungscharakteristiken im Bereich um den V-Kerb beobachtet. Die zweite Methode bietet eine vollstaendige Erhoehungsabbildung der Bruchoberflaeche durch Extraktion der Fokusierungsbereiche

  6. [Peter von Brackel. Christlich Gesprech von der grawsamen Zerstörung in Lifland durch den Muscowiter vom 58. Jar her geschehenn...] / Stefan Donecker

    Index Scriptorium Estoniae

    Donecker, Stefan, 1977-

    2015-01-01

    Arvustus: Christlich Gesprech von der grawsamen Zerstörung in Lifland durch den Muscowiter vom 58. Jar her geschehenn: auch ihren Ursachen mit einer kurtzen Predig und Vermanung, wie beid, Gotlosenn unnd Frommen, diese schreckliche Mutation fruchtbarlich behertzigen und ihnen zu Nutz machen sollen: durch Timannum Brakel Livoniensem, der Gemeine Christi vonn der Augsburgischen Confession Prediger zu Anttorf einfeltig gestellet unnd inn Druck verfertiget = Darstellung der Geschichte Livlands vor und während des "Livländischen Krieges" bis 1578 in gereimter Dialogform und Prosa von dem Prediger und Geschichtsschreiber Timann Brakel : Originaltext und Übersetzung ins Hochdeutsche mit Kommentaren, Ergänzungen, Bildern und dem Lebenslauf des Timann Brakel. (Beiträge zur baltischen Geschichte, 19). Verlag Harro von Hirschheydt. Wedemark 2012

  7. [Peter von Brackel. Christlich Gesprech von der grawsamen Zerstörung in Lifland durch den Muscowiter vom 58. Jar her geschehenn...] / Stefan Donecker

    Index Scriptorium Estoniae

    Donecker, Stefan, 1977-

    2015-01-01

    Arvustus: Christlich Gesprech von der grawsamen Zerstörung in Lifland durch den Muscowiter vom 58. Jar her geschehenn: auch ihren Ursachen mit einer kurtzen Predig und Vermanung, wie beid, Gotlosenn unnd Frommen, diese schreckliche Mutation fruchtbarlich behertzigen und ihnen zu Nutz machen sollen: durch Timannum Brakel Livoniensem, der Gemeine Christi vonn der Augsburgischen Confession Prediger zu Anttorf einfeltig gestellet unnd inn Druck verfertiget = Darstellung der Geschichte Livlands vor und während des "Livländischen Krieges" bis 1578 in gereimter Dialogform und Prosa von dem Prediger und Geschichtsschreiber Timann Brakel : Originaltext und Übersetzung ins Hochdeutsche mit Kommentaren, Ergänzungen, Bildern und dem Lebenslauf des Timann Brakel. (Beiträge zur baltischen Geschichte, 19). Verlag Harro von Hirschheydt. Wedemark 2012

  8. Post-treatment of hydrocarbon-burdened effluents by means of adsorption to bentonites; Nachbehandlung kohlenwasserstoffhaltiger Abwaesser durch Adsorption an Bentonite

    Energy Technology Data Exchange (ETDEWEB)

    Breuker, F. [Fachhochschule Muenster, Burgsteinfurt (Germany). Fachbereich Chemieingenieurwesen; Nussbaumer, A. [Edelhoff Entsorgung West GmbH und Co., Hagen (Germany)

    1996-06-01

    During chemical/physical treatment of liquid toxic waste, e.g. emulsions or oil/water mixtures, a separate process stage of hydrocarbon adsorption may be necessary after hydroxide precipitation of the metal salts as soon as the metal freight is reduced by the application of anaerobic biological processes. This is possible, in principle, with the aid of bentonites. Instead of the discontinuous process investigated here, a continuous process would be better suited in practice. (orig.) [Deutsch] Bei der chemisch-physikalischen Behandlung fluessiger Sonderabfaelle wie Emulsionen oder Oel-Wasser-Gemischen kann nach einer Hydroxidfaellung der Metallsalze ein separates Verfahren zur Adsorption von Kohlenwasserstoffen erforderlich sein, sobald die Metallfracht durch den Einsatz einer anaeroben Biologie reduziert wird. Dies ist durch den Einsatz von Bentoniten grundsaetzlich moeglich. Anstelle der hier untersuchten diskontinuierlichen Verfahrensweise waere fuer die betriebliche Praxis ein kontinuierliches Verfahren guenstiger. (orig.)

  9. Climate protection by reducing the emissions of greenhouse gases in households and the tertiary sector through climate-conscious behaviour. Vol. 1; Klimaschutz durch Minderung von Treibhausgasemissionen im Bereich Haushalte und Kleinverbrauch durch klimagerechtes Verhalten. Bd. 1. Private Haushalte

    Energy Technology Data Exchange (ETDEWEB)

    Brohmann, B.; Cames, M.

    2000-06-01

    The aim of the project was to identify areas in households and the tertiary sector in which changes in behaviour could result in energy conservation and thus a reduction of greenhouse gas emissions, and to quantify the potentials for 1995, 2005 and 2020. A second focus was on the analysis and evaluation of programmes and instruments to realise the potentials. With literature evaluation, expert interviews, and a household servey potentials and further technical development have been identified. In sum, behavioural measures can contribute to the CO2 reduction by 64 million tons in 1995 in households and 27 in the commercial sector in which the potential decreases to 18 million tons in 2020 due to the autonomous technical development. Adequate promotion programmes can help to realise 20-30% of the potential by 2020. (orig.) [German] Ziel des Vorhabens war, im Sektor private Haushalte und Kleinverbrauch Bereiche zu identifizieren, in denen Verhaltensaenderungen zur Energieeinsparung fuehren koennen, und diese Potenziale fuer 1995, 2005 und 2020 zu quantifizieren. Darauf aufbauend waren Programme und Instrumente zur Umsetzung aufzuzeigen und zu bewerten. Gestuetzt auf Literaturrecherchen und Expertengespraeche wurden Einzelpotenziale, Rahmenbedingungen, Entwicklungstrends in der Technik und im Ausstattungsgrad ermittelt. Insgesamt koennten Verhaltensmassnahmen im Haushaltssektor die CO2-Emissionen im Basisjahr 1995 um 64 Mio, im Kleinverbrauch um 27 Mio t vermindern. Bis 2020 bleibt dieses Potenzial im Haushaltssektor in etwa gleich. Im Kleinverbrauch sinkt es infolge der autonomen Technikentwicklung auf 18 Mio t ab. Durch geeignete Programme koennen bis 2020 etwa 20-30% des Potenzials erreicht werden. (orig.)

  10. Replacement of coal-fuelled stoves by modern room heating systems in multistorey dwellings; Abloesung der Kohle-Einzelofenheizung durch moderne Raumheizungssysteme in Mehrgeschossbauten

    Energy Technology Data Exchange (ETDEWEB)

    Lindner, K. [Institut fuer Energetik und Umwelt gGmbH, Leipzig (Germany)

    1997-12-31

    In eastern Germany, approximately one million residential units in multi-storey apartment buildings are still heated with coal-fired stoves. Not all building societies or private house-owners can afford to substitute modern heating systems for individual coal stoves within the foreseeable future. Problems encountered when modernizing heating systems are described. A way of pre-financing modernization is by loans given by the tenants to the building society. The paper deals with individual aspects, such as contract conditions, organization and experience with this model, as well as its advantages for the landlord and tenant. (MSK) [Deutsch] In Ostdeutschland werden noch rund 1 Million Wohnungen in mehrgeschossigen Wohnhaeuern mit Kohle geheizt. Nicht alle Wohnungsgenossenschaften oder privaten Hauseigentuemer sind finanziell in der Lage in absehbarer Zeit die Kohle-Einzelheizung durch moderne Heizungssysteme abzuloesen. Im Folgenden werden die Probleme bei der Heizungsmodernisierung beschrieben. Der Weg Heizungsmodernisierung durch Mieterdarlehen wird in einzelnen Punkten wie Finanzierung durch Mieterdarlehen, deren Organisation und Erfahrungen mit diesem Modell sowie die Vorteile fuer Vermieter und Mieter dargelegt.

  11. Ischemia of the lower limb by a persistent sciatic artery - a potential interventional therapy approach; Ischaemie der unteren Extremitaet durch eine persistierende A. ischiadica - ein moeglicher interventioneller Therapieansatz

    Energy Technology Data Exchange (ETDEWEB)

    Tomczak, R.; Goerich, J.; Brambs, H.J. [Ulm Univ. (Germany). Klinik und Poliklinik fuer Radiologie; Pamler, R. [Ulm Univ. (Germany). Klinik fuer Gefaesschirurgie

    2000-08-01

    The sciatic artery is a rarely seen variant of the vessels of the lower limb. Anatomically it is the prolongation of the inferior gluteal artery and accompanies the sciatic nerve from the sciatic foramen to the knee. More than other vessels, the sciatic artery shows vasculopathies like diffuse dilatation or aneurysms. This report describes an interesting case of embolic acute ischemia, caused by a persistent sciatic artery with an aneurysm. We treated the ischemia with intraA.I lysis and bridged the aneurysm with an covered stent. (orig.) [German] Die A. ischiadica ist eine selten zu findende anatomische Variante des Gefaesssystems der unteren Extremitaet. Anatomisch entspricht die A. ischiadica einer Verlaengerung der A. glutea inferior und begleitet den N. ischiadicus ab dem Foramen ischiadicum bis zum Knie, wo sie in die Unterschenkelarterien muendet und zu den muskelversorgenden Arterien gehoert. Die A. ischiadica neigt aufgrund eines minderwertigen Gefaessaufbaus zu Vaskulopathien. Insbesondere aneurysmale Dilatationen der A. ischiadica sind mit einer hohen Inzidenz beschrieben worden. Der vorliegende Fallbericht beschreibt den interessanten Fall einer akuten Ischaemie im Bereich des Unterschenkels, ausgeloest durch Thromben aus einer aneurysmatisch veraenderten A. ischiadica. Die akute Ischaemie wurde erfolgreich durch eine intraarterielle Lyse behandelt. Das Aneurysma wurde sekundaer durch eine beschichtete Stentprothese ausgeschaltet. (orig.)

  12. Karyomorphological Studies in Some Species of Parnassia L. (Saxifragaceae s.l. in East Asia and Intraspecific Polyploidy of P. palustris L.

    Directory of Open Access Journals (Sweden)

    Tsuneo Funamoto

    2012-01-01

    Full Text Available Karyomorphological information is one of the most important characters for cytotaxonomy. We described karyomorphology of 14 species of Parnassia in East Asia. They had commonly the resting chromosomes of the simple chromocenter type and the mitotic prophase chromosomes of the proximal type. The somatic chromosome number of 2n=14 was shown in three species, that of 2n=18 was shown in six species, that of 2n=18 or 36 was shown in two species, that of 2n=32 was shown in one species, that of 2n=36 or 36+1∼8 s was shown in one species, and that of 2n=18, 27, 36 or 45 was shown in one species. They were commonly monomodal (gradual decrease in length from the largest to the smallest chromosomes. However, their absolute chromosome sizes were different from each other. Average chromosome sizes were recognized as three types small (1.21 μm, medium (1.7–2.36 μm, and large (3.1–3.75 μm. Parnassia palustris showed four cytotypes that could be likely cytogeographically correlated with habitats, polyploidy, and sizes of pollen grains.

  13. High-resolution bioactivity profiling combined with HPLC-HRMS-SPE-NMR: α-Glucosidase inhibitors and acetylated ellagic acid rhamnosides from Myrcia palustris DC. (Myrtaceae).

    Science.gov (United States)

    Wubshet, Sileshi G; Moresco, Henrique H; Tahtah, Yousof; Brighente, Inês M C; Staerk, Dan

    2015-08-01

    Type 2 diabetes (T2D) is an endocrine metabolic disease with a worldwide prevalence of more than 8%, and an expected increase close to 50% in the next 15-20years. T2D is associated with severe and life-threatening complications like retinopathy, neuropathy, nephropathy, and cardiovascular diseases, and therefore improved drug leads or functional foods containing α-glucosidase inhibitors are needed for management of blood glucose. In this study, leaves of Myrcia palustris were investigated by high-resolution α-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR. This led to identification of casuarinin, myricetin 3-O-β-d-(6″-galloyl)galactopyranoside, kaempferol 3-O-β-d-galactopyranoside, myricetin, and quercetin as α-glucosidase inhibitors. In addition, four acetylated ellagic acid rhamnosides, i.e., 4-O-(2″,4″-O-diacetyl-α-l-rhamnopyranosyl)ellagic acid, 4-O-(2″,3″-O-diacetyl-α-l-rhamnopyranosyl)ellagic acid, 4-O-(3″,4″-O-diacetyl-α-l-rhamnopyranosyl)ellagic acid, and 4-O-(2″,3″,4″-O-triacetyl-α-l-rhamnopyranosyl)ellagic acid were identified.

  14. Advantages of variable-speed operation of hydraulic turbo-engines; Vorteile durch den drehzahlvariablen Betrieb von hydraulischen Stroemungsmaschinen

    Energy Technology Data Exchange (ETDEWEB)

    Harbort, T. [Stuttgart Univ. (Germany). Inst. fuer Stroemungsmechanik und Hydraulische Stroemungsmaschinen

    1997-12-31

    The performance of current hydraulic turbo-engines in the variable speed sector is monitored and judged. The study covers radial and axial engines as well as Pelton turbines. Variable-speed operation of hydraulic turbo-engines can be realized by means of different combinations of electrical rotating machines and frequency converters. The operating range of the frequency converter plays an important role in the optimization of performance and is taken into account. The smoothness of run of reaction turbines and their cavitation performance can be enhanced by speed regulation. But above all, efficiency is more or less substantially enhanced during partial load or in the case of greatly varying heights of drop. The latter holds true also of Pelton turbines. (orig.) [Deutsch] Das Betriebsverhalten der gaengigen hydraulischen Stroemungsmaschinen wird in Hinblick auf den drehzahlvariablen Betrieb erfasst und beurteilt. Die Untersuchung erfolgt fuer Radialmaschinen, Axialmaschinen und Peltonturbinen. Der drehzahlvariable Betrieb hydraulischer Stroemungsmaschinen kann mit verschiedenen Kombinationen von elektrischen Maschinen und Frequenzumrichtern realisiert werden. Der Arbeitsbereich des Frequenzumrichters spielt eine wichtige Rolle fuer die Optimierung des Betriebsverhaltens und wird beruecksichtigt. Bei Ueberdruckturbinen kann man durch Drehzahlregelung eine groessere Laufruhe sowie ein guenstigeres Kavitationsverhalten erreichen. Vor allem aber sind im Teillastbereich oder bei stark schwankenden Fallhoehen mehr oder weniger grosse Wirkungsgradgewinne erzielbar. Das letztere gilt auch fuer Peltonturbinen. (orig.)

  15. Altitude dependence of trace substance deposition from clouds to forests. Final report; Hoehenabhaengigkeit der Spurenstoffdeposition durch Wolken auf Waelder. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Pahl, S.; Winkler, P.

    1995-12-31

    Novel forest decline is particularly pronounced in the area of the ridges of medium-range mountains. Whereas acid precipitation was viewed as its sole cause early on in the discussions, it turned out later that the impact of trace gases, too, contributes to the damaging of forests. This report wants to point out the importance of fog interception, which equally plays a part in the pollutant receipts of forests. The deposition of fog water to a forest stand depends very much on altitude, so that trace substance deposition, too, is to be expected to be dependent on altitude. By attempting to quantify this effect, the report helps to pinpoint areas of relevance of this deposition pathway (orig./KW) [Deutsch] Die neuartigen Waldschaeden sind in den Kammlagen der Mittelgebirge besonders ausgepraegt. Waehrend in der anfaenglichen Diskussion die sauren Niederschlaege als alleinige Ursache angesehen wurden, zeigte sich spaeter, dass auch Einwirkungen von Spurengasen zur Schaedigung des Waldes beitragen. Dieser Bericht soll auf die Bedeutung der Nebelinterzeption aufmerksam machen, die ebenfalls zum Schadstoffeintrag in den Wald beitraegt. Die Deposition von Wolkenwasser auf einen Waldbestand ist stark abhaengig von der Hoehenlage, in der sich der Waldbestand befindet, so dass auch eine Hoehenabhaengigkeit des Spurenstoffeintrages zu erwarten ist. Durch den Versuch der Quantifizierung traegt dieser Bericht dazu bei, Gebiete zu erkennen, in denen dieser Eintragspfad eine Rolle spielt. (orig./KW)

  16. Neue Möglichkeiten in der archäologischen arbeit durch den Einsatz digitales Bildauswertung und photogrammetrischer Messtechniken

    Directory of Open Access Journals (Sweden)

    Jan Wesbuer

    2005-01-01

    Full Text Available Die digitale Bildbearbeitung und die digitale Photogrammetrie haben in den letzten Jahren, neben vielen anderen Gebieten, die Dokumentation von Ausgrabungsstätten beeinflusst. Die Photogrammetrie hat ihren Ursprung in der Vermessungskunde. Sie wird heutzutage in vielen Bereichen der Ingenieurwissenschaften eingesetzt und hat somit viele Schnittbereiche zu angrenzenden wissenschaftlichen Gebieten. In der Archäologie hat sie daher die Möglichkeit, in vielen Bereichen die herkömmliche Geodäsie zu erweitern bzw. neue Möglichkeiten der Visualisierung zu schaffen oder bei der Bildauswertung zu helfen.Durch die Bildbearbeitung ist es heutzutage möglich, schnell und vor Ort die Dokumentation vorzunehmen. Mit ihrer Hilfe können z.B. nicht orthogonale Aufnahmen perspektivisch entzerrt werden, so dass in ihnen wieder gemessen werden kann.Der Artikel unterteilt sich in 3 Bereiche:• als erstes werden die Möglichkeiten der digitalen Bildbearbeitung bzw. Auswertung von Photos dargestellt.• danach wird auf die photogrammetrische Auswertung und Vermessung von Ausgrabungsstätten eingegangen. • und zuletzt werden einige Visualisierungsmöglichkeiten gezeigt, die mit Hilfe der photogrammetrischen Auswertung und 3DModellerstellung realisiert werden können.

  17. Retrospektive Analyse von Zufallsbefunden, die bei Patienten mit kutanem malignen Malignom durch (18) F-Fluordeoxyglucose-PET/CT erhoben wurden.

    Science.gov (United States)

    Conrad, Franziska; Winkens, Thomas; Kaatz, Martin; Goetze, Steven; Freesmeyer, Martin

    2016-08-01

    Bei der (18) F-Fluordeoxyglucose-Positronenemissionstomographie/Computertomographie (FDG-PET/CT) ergeben sich häufig Zufallsbefunde. In der vorliegenden Studie konzentrierten wir uns auf mittels FDG-PET/CT erhaltene Zufallsbefunde bei Patienten mit kutanem Melanom und überprüften deren Relevanz hinsichtlich weiterer diagnostischer Maßnahmen und Interventionen. Die Krankenakten von 181 konsekutiven Melanom-Patienten wurden retrospektiv ausgewertet, um das Management von Zufallsbefunden zu dokumentieren. Der Schwerpunkt lag dabei auf den histologischen Befunden. Bei 33 von 181 (18 %) Patienten lagen 39 relevante Zufallsbefunde vor, und zwar im Kolorektalbereich (n = 15 Patienten), in der Schilddrüse (n = 8), der Prostata (n = 2), dem Bewegungsapparat (n = 2), in Lymphknoten (n = 2), der Parotis (n = 1), den Mandeln (n = 1), den Nieren (n = 1) und der Gallenblase (n = 1). Bei 25 Patienten schlossen sich weitere diagnostische Verfahren an, wobei in 21 Fällen ein klinisches Korrelat nachgewiesen wurde. Bei 16 von 21 Patienten ergab sich eine Neoplasie, darunter fünf maligne Läsionen (vier Kolonkarzinome und ein Prostatakarzinom). Die Malignome wurden frühzeitig diagnostiziert und in der Mehrzahl der Fälle erfolgreich entfernt. Der Einsatz der FDG-PET/CT als Routine-Diagnostik wird in den Leitlinien empfohlen und ist indiziert bei malignem Melanom ab Stadium IIC. In dieser Studie wurden auf effektive Weise ansonsten nicht erkannte Krebserkrankungen, insbesondere Kolonkarzinome, detektiert. In den meisten Fällen war eine frühe Intervention möglich. Zufallsbefunde durch FDG-PET/CT sollten, unter Berücksichtigung des Zustands und der Wünsche des Patienten, mit den geeigneten diagnostischen Maßnahmen abgeklärt werden. © 2016 Deutsche Dermatologische Gesellschaft (DDG). Published by John Wiley & Sons Ltd.

  18. Potential of energy conservation in heating systems by means of blower convectors; Energieeinsparungspotential in Heizungsystemen durch den Einsatz von Geblaesekonvektoren

    Energy Technology Data Exchange (ETDEWEB)

    Binder, Andreas; Raisch, Ingo

    2011-07-01

    Blowers are used for heating in buildings, mostly under windows, in corners, or in niches. When heating systems are modernized, it is a useful strategy to reduce the flow temperature, but this means that the existing radiator is underdimensioned. A convective blower is presented that fits into most heating niches and provides the same capacity at lower water temperatures. Disadvantages are electric power consumption, higher noise, and faster soiling. Advantages are fast and comfortable room temperature control and the possibility of cooling. Theoretical assessments are made to investigate the potential savings resulting from convective blowers as well as their effects in terms of comfort and air quality. [German] In Gebaeuden werden zur Raumheizung in vielen Faellen Radiatoren eingesetzt. Diese werden beispielsweise unter Fenstern oder in Ecken platziert. Der Architekt sieht fuer den Einbau der Geraete oftmals eine Nische vor. Wird das komplette Heizungssystem saniert, ist es unter energetischen Gesichtspunkten sinnvoll die Vorlauftemperatur zu reduzieren. Der vorhandene Heizkoerper ist damit unterdimensioniert. Es wird ein Geblaesekonvektor entwickelt, der in eine Vielzahl der architektonisch ueblichen Heiznischen passt. Bei Abgabe der gleichen thermischen Leistung, ist es mit diesem Geraet moeglich die Heizung bei geringeren Wassertemperaturen zu betreiben. Die Beheizung von Raeumen mittels Konvektoren ist aber auch mit einigen Nachteilen behaftet, so wird elektrische Energie benoetigt, die Geraete emittieren Schall und neigen staerker zur Verschmutzung. Dem gegenueber stehen einige Vorteile wie einer schnelleren und komfortableren Regelung der Raumtemperatur und die Moeglichkeit zu Kuehlen. Es werden theoretische Abschaetzungen durchgefuehrt, wie gross das Einsparpotential durch Geblaesekonvektoren sein kann, und wie sich die Umstellung auf den Komfort, und die Luftqualitaet auswirkt.

  19. Potential and possibilities of supplying energy from biomass and biogas; Potentiale und Moeglichkeiten der Energiebereitstellung durch Biomasse und Biogas

    Energy Technology Data Exchange (ETDEWEB)

    Sonnenberg, H. [Bundesforschungsanstalt fuer Landwirtschaft, Braunschweig (Germany). Inst. fuer Betriebstechnik; Weiland, P.; Ahlgrimm, H.J. [Bundesforschungsanstalt fuer Landwirtschaft (FAL), Braunschweig (Germany). Inst. fuer Technologie

    1998-06-01

    Agriculture`s potential contribution to the energy supply of the ``town of the future`` through the conversion of biomass to energy, including biogas production, is a rather modest one. Supposing that the share of total renewable energy in Germany`s primary energy demand rises to approximately 4%, then the proportion of biomass from biotic raw materials especially produced for the purpose will at the most make up an eighth of this amount. Beyond this, biomass is burdened with other drawbacks such as low supply efficiency, limited availability, and weather-dependent reliability. On the other hand, biomass is well suited for conversion to solid, liquid, and gaseous fuels, including inexpensive ones with low energy density (solid fuels), mostly used for stationary heating applications, as well as more expensive ones such as liquid fuels with a high energy density for mobile applications in the automotive sector. Thanks to its capacity to regenerate, biomass is an inexhaustible resource. Moreover, its natural life cycle has a small impact on the environment. [Deutsch] Der Beitrag, den die Landwirtschaft durch energetische Nutzung von Biomasse, z.B. auch mit der Erzeugung von Biogas, zur Energieversorgung der `Stadt der Zukunft` leisten kann, nimmt sich bescheiden aus. Wird erwartet, dass innerhalb des naechsten Jahrzehnts der Anteil regenerativer Energien insgesamt auf etwa 4% des Primaerenergie-Verbrauchs Deutschlands ansteigen koennte, so duerfte Biomasse als speziell zur Energiegewinnung angebaute nachwachsende Rohstoffe mit bestensfalls 0,5 Prozentpunkten daran beteiligt sein. Es beduerfen darueber hinaus auch Nachteile, wie geringe Bereitstellungseffizienz, beschraenkte Verfuegbarkeit und witterungsabhaengige Zuverlaessigkeit, der Beachtung. Die Biomasse kann jedoch mit Erfolg in feste, fluessige und gasfoermige Energietraeger konvertiert werden, sowohl in preiswerte mit geringer Energiedichte (Festbrennstoffe) fuer bevorzugt stationaeren Heizungs-Einsatz als auch

  20. Micorrizas arbusculares asociadas a Júcaro de ciénaga ( Bucida palustris y Soplillo ( Lysiloma latisiliquum en la Reserva de la Biosfera Ciénaga de Zapata, Cuba.

    Directory of Open Access Journals (Sweden)

    Raquel M. Rodríguez Rodríguez

    2014-01-01

    Full Text Available Se evaluó y caracterizó el e status micorrízico arbuscular de Bucida palustris (j úcaro de ciénaga y Lysiloma latisiliquum (soplillo , especies distintivas de bosque de ciénaga y bosque semidecíduo con humedad fluctuante, respectivamente, en la Reserva de la Biosfera Ciénaga de Zapata . Se determinó además, la densidad de esporas rizosféricas de hongos micorrizógenos arbusculares y se identificó la especie predominante en cada caso. Ambas especies vegetales resultaron micótrofas arbusculares con elevados porcentajes de colonización (> 9 0 % y densidad visual ( >25 %. La densidad de esporas rizosféricas asociadas a Lysiloma latisiliquum fue casi 2,5 veces mayor que en Bucida palustris y las especies predominantes fueron Funneliformis halonatus y Racocetra alborosea , respectivamente. La m orfología Tipo - Paris se identificó en Lysiloma latisiliquum y el Tipo - Arum en Bucida palustris . Se observaron además de los enrollados hifales y arbúsculos determinantes de los tipos morfológicos anteriormente descritos, esporas y vesículas intrarradicales en ambas especies. Este trabajo constituye el primer reporte de micorrizas arbusculares asociadas a especies vegetales en humedales cubanos. Estudios acerca de su funcionamiento y diversidad, así como su relación con plantas cubanas permitirá una mejor co mprensión de esta simbiosis y facilitará la utilización de micorrizas nativas en estrategias de conservación y uso sostenible de plantas y ecosistemas.

  1. Genome Assembly of the Fungus Cochliobolus miyabeanus, and Transcriptome Analysis during Early Stages of Infection on American Wildrice (Zizania palustris L..

    Directory of Open Access Journals (Sweden)

    Claudia V Castell-Miller

    Full Text Available The fungus Cochliobolus miyabeanus causes severe leaf spot disease on rice (Oryza sativa and two North American specialty crops, American wildrice (Zizania palustris and switchgrass (Panicum virgatum. Despite the importance of C. miyabeanus as a disease-causing agent in wildrice, little is known about either the mechanisms of pathogenicity or host defense responses. To start bridging these gaps, the genome of C. miyabeanus strain TG12bL2 was shotgun sequenced using Illumina technology. The genome assembly consists of 31.79 Mbp in 2,378 scaffolds with an N50 = 74,921. It contains 11,000 predicted genes of which 94.5% were annotated. Approximately 10% of total gene number is expected to be secreted. The C. miyabeanus genome is rich in carbohydrate active enzymes, and harbors 187 small secreted peptides (SSPs and some fungal effector homologs. Detoxification systems were represented by a variety of enzymes that could offer protection against plant defense compounds. The non-ribosomal peptide synthetases and polyketide synthases (PKS present were common to other Cochliobolus species. Additionally, the fungal transcriptome was analyzed at 48 hours after inoculation in planta. A total of 10,674 genes were found to be expressed, some of which are known to be involved in pathogenicity or response to host defenses including hydrophobins, cutinase, cell wall degrading enzymes, enzymes related to reactive oxygen species scavenging, PKS, detoxification systems, SSPs, and a known fungal effector. This work will facilitate future research on C. miyabeanus pathogen-associated molecular patterns and effectors, and in the identification of their corresponding wildrice defense mechanisms.

  2. Separation of ammonia from aqueous solutions by diffusion through membranes. Final report; Abtrennung von Ammoniak aus waessrigen Loesungen durch Membrandiffusion. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Weidlich, H.G.; Friedrich, G. [Ingenieurgesellschaft fuer Energie- und Kraftwerkstechnik mbH (IEK), Cottbus (Germany); Schwarzer, F. [SEP Gesellschaft fuer Technische Studien, Entwicklung, Planung mbH, Ismaning (Germany)

    2001-04-01

    The separation of ammonia from aqueous solutions by diffusion through membranes comprises the diffusion of gaseous ammonia through the pores of a non-wettable membrane into an acid, in which it is converted to non-volatile ammonium. For an effective separation of ammonia the pH-value and/or the temperature have to be increased to such a degree, that the ammonium, contained in the waste-water, is converted mainly to ammonia. Experimental plants were constructed on the basis of process parameters determined by laboratory tests, and tested for the treatment of liquid manure and of waste-water from power plants. The separation of ammonia from liquid manure was possible but couldn't be performed economically. On the other hand the treatment of waste-water from power plants seems to be promising also economically. In comparison with the stripping processes the membrane diffusion is characterized by a very low energy consumption and economical advantages specially at small capacities. (orig.) [German] Zur Abtrennung von Ammoniak aus waessrigen Loesungen durch Membrandiffusion diffundiert gasfoermiges Ammoniak durch die Poren einer nicht benetzbaren Membran in eine Saeure, in der es zu nicht fluechtigen Ammoniak umgesetzt wird. Zur wirkungsvollen Abtrennung von Ammoniak muessen pH-Wert und/oder die Temperatur soweit angehoben werden, dass das im Abwasser enthaltene Ammonium zum groessten Teil in Ammoniak umgewandelt wird. Nach Ermittlung geeigneter Verfahrensparameter in Laborversuchen wurden Versuchsanlagen zur Aufbereitung von Guelle und Abwaessern aus den Kraftwerksbereich erprobt. Die Ammoniakabtrennung aus Guelle erwies sich dabei zwar als moeglich, aber nicht als auf wirtschaftliche Weise durchfuehrbar. Die Aufbereitung von Kraftwerksabwaessern erscheint dagegen auch in wirtschaftlicher Hinsicht als interessant. Im Vergleich zu den Strippverfahren zeichnet sich die Membrandiffusion durch einen sehr geringen Energieeinsatz aus und weist in wirtschaftlicher

  3. Reduction of exhaust gas and sound emissions of diesel trolleys by alternative drives; Verminderung der Abgas- und Schallemissionen von Dieselkatzen durch alternative Antriebe

    Energy Technology Data Exchange (ETDEWEB)

    Wehner, G. [Deutsche Montan Technologie GmbH, Bochum (Germany). Geschaeftsbereich Car Synergies Div.

    2001-08-02

    It was to be investigated within the framework of a research and development project whether it is possible to replace the diesel hydraulic power transmission in diesel trolleys by diesel electric power. The aim was thus to increase the efficiency, reduce the maintenance costs and also increase the maximum speed. (orig.) [German] Im Rahmen eines Forschungs- und Entwicklungsvorhabens sollte untersucht werden, ob es moeglich ist, die dieselhydraulische Kraftuebertragung bei Dieselkatzen durch eine dieselelektrische zu ersetzen. Somit sollten eine Vergroesserung des Wirkungsgrads und eine Verminderung des Wartungsaufwands erreicht werden. Auch eine Erhoehung der Hoechstgeschwindigkeit wurde angestrebt. (orig.)

  4. Hautreizungen durch Propylenglykol

    DEFF Research Database (Denmark)

    Andersen, Klaus Ejner; Storrs, F J

    1982-01-01

    Propylene glycol is used in foods, medicine and cosmetics because it is a good solvent which has, simultaneously, moisture-regulating, antiseptic, and preservative effects. Propylene glycol may produce eczematous skin reactions of toxic and, more rarely, of allergic nature. Positive patch test...... reactions to propylene glycol are difficult to interpret. Allergic reactions may be confirmed by a clear clinical relevance, repeated local skin provocation (usage test), or oral provocation. In the Department of Dermatology, University of Oregon, 84 patients were patch tested with 100% propylene glycol...... with positive reactions to patch tests showed an itchy eczematous eruption after oral provocation with 15 ml propylene glycol. Skin reactions due to propylene glycol are rare and should not bring the preparation into unnecessary discredit. The possibility of propylene glycol allergy should be recognized...

  5. Hautreizungen durch Propylenglykol

    DEFF Research Database (Denmark)

    Andersen, Klaus Ejner; Storrs, F J

    1982-01-01

    Propylene glycol is used in foods, medicine and cosmetics because it is a good solvent which has, simultaneously, moisture-regulating, antiseptic, and preservative effects. Propylene glycol may produce eczematous skin reactions of toxic and, more rarely, of allergic nature. Positive patch test...... reactions to propylene glycol are difficult to interpret. Allergic reactions may be confirmed by a clear clinical relevance, repeated local skin provocation (usage test), or oral provocation. In the Department of Dermatology, University of Oregon, 84 patients were patch tested with 100% propylene glycol....... Five of 12 patch test-positive patients had allergic reactions while seven had irritant reactions. In the Department of Dermatology, Gentofte Hospital, 248 consecutive eczema patients were patch-tested with propylene glycol in concentrations of 100%, 20%, and 2% in water. Two of five patients...

  6. Durch das Leben lernen

    OpenAIRE

    Ingolf Waßmann; Daniel Versick; Anja Thomanek; Djamshid Tavangarian

    2014-01-01

    Web 2.0 und soziale Netzwerke gaben erste Impulse für neue Formen der Online-Lehre, welche die umfassende Vernetzung von Objekten und Nutzern im Internet nachhaltig einsetzen. Die Vielfältigkeit der unterschiedlichen Systeme erschwert aber deren ganzheitliche Nutzung in einem umfassenden Lernszenario, das den Anforderungen der modernen Informationsgesellschaft genügt. In diesem Beitrag wird eine auf dem Konnektivismus basierende Plattform für die Online-Lehre namens “Wiki-Learnia” präsentiert...

  7. Long-time preservation of biologic cells through cold application: basic processes, influence quantities, and process optimation; Langzeitkonservierung biologischer Zellen durch Kaelteanwendung: Grundlegende Vorgaenge, Einflussparameter und Verfahrensoptimierung

    Energy Technology Data Exchange (ETDEWEB)

    Heschel, I. [Helmholtz-Inst. fuer Biomedizinische Technik an der RWTH Aachen (Germany); Sputtek, A. [Helmholtz-Inst. fuer Biomedizinische Technik an der RWTH Aachen (Germany); Nunner, B. [Helmholtz-Inst. fuer Biomedizinische Technik an der RWTH Aachen (Germany); Rau, G. [Helmholtz-Inst. fuer Biomedizinische Technik an der RWTH Aachen (Germany)

    1994-12-31

    In diverse fields of human medicine there is a demand for cryopreserved biologic cells. Cooling cells down to temperatures in the range of -80 C to -196 C brings their entire metabolism to a virtual standstill, so that they can then be preserved for longer periods of time in the vital state. Development of successful cryopreservation methods presupposes an exact knowledge of the diverse mechanisms of cell impairment through freezing (e.g. osmotic stress, intracellular ice formation). In order to achieve sufficient cell survival rates at technically feasible cooling rates it is necessary to use cryoprotective additives (e.g. dimethyl sulphoxide, hydroxyethyl starch). Process optimation is indispensable and involves precise coordination of the influence parameters cell type and concentration; type and composition of the protective additive; and cooling and warming rate. (orig.) [Deutsch] In den verschiedensten Bereichen derr Humanmedizin besteht Bedarf fuer kryokonservierte biologische Zellen. Durch die Abkuehlung der Zellen auf Temperaturen im Bereich von -80 C bis -196 C kommen die Stoffwechselvorgaenge praktisch zum Stillstand, so dass die Zellen bei Erhalt ihrer Vitalitaet langzeitgelagert werden koennen. Zur Entwicklung erfolgreicher Kryokonservierungsverfahren ist die genaue Kenntnis der vielfaeltigen Zellschaedigungsmechanismen beim Einfrieren (z.B. osmotische Belastung, intrazellulaere Eisbildung) erforderlich. Erst durch den Zusatz von Kryoprotektiven (z.B. Dimethylsulfoxid, Hydroxyethylstaerke) gelingt die Kryokonservierung der Zellen in ausreichender Anzahl bei technisch realisierbaren Kuehlraten. Bei der Verfahrensoptimierung ist die genaue Abstimmung der Einflussparameter Zellart und -konzentration, Schutzadditivart und -zusammensetzung sowie Kuehl- und Erwaermungsrate zwingend erforderlich. (orig.)

  8. Long-time preservation of biologic cells through cold application: basic processes, influence quantities, and process optimation; Langzeitkonservierung biologischer Zellen durch Kaelteanwendung: Grundlegende Vorgaenge, Einflussparameter und Verfahrensoptimierung

    Energy Technology Data Exchange (ETDEWEB)

    Heschel, I. [Technische Hochschule Aachen (Germany). Helmholtz-Inst. fuer Biomedizinische Technik; Sputtek, A. [Technische Hochschule Aachen (Germany). Helmholtz-Inst. fuer Biomedizinische Technik; Nunner, B. [Technische Hochschule Aachen (Germany). Helmholtz-Inst. fuer Biomedizinische Technik; Rau, G. [Technische Hochschule Aachen (Germany). Helmholtz-Inst. fuer Biomedizinische Technik

    1994-12-31

    In diverse fields of human medicine there is a demand for cryopreserved biologic cells. Cooling cells down to temperatures in the range of -80 C and -196 C brings their entire metabolism to a virtual standstill, so that they can then be preserved for longer periods in the vital state. Development of successful cryopreservation methods presupposes an exact knowledge of the diverse mechanisms of cell impairment through freezing (e.g. osmotic stress, intracellular ice formation). In order to achieve sufficient cell survival rates at technically feasible cooling rates if is necessary to use cryoprotective additives (e.g. dimethyl sulphoxide, hydroxyethyl starch). Process optimation is indispensable and involves precise coordination of the influence parameters cell type and concentration; type and composition of the protective additive; and cooling and warming rate. (orig.) [Deutsch] In den verschiedensten Bereichen der Humanmedizin besteht Bedarf fuer kryokonservierte biologische Zellen. Durch die Abkuehlung der Zellen auf Temperaturen im Bereich von -80 C bis -196 C kommen die Stoffwechselvorgaenge praktisch zum Stillstand, so dass die Zellen bei Erhalt ihrer Vitalitaet langzeitgelagert werden koennen. Zur Entwicklung erfolgreicher Kryokonservierungsverfahren ist die genaue Kenntnis der vielfaeltigen Zellschaedigungsmechanismen beim Einfrieren (z.B. osmotische Belastung, intrazellulaere Eisbildung) erforderlich. Erst durch den Zusatz von Kryoprotektiven (z.B. Dimethylsulfoxid, Hydroxyethylstaerke) gelingt die Kryokonservierung der Zellen in ausreichender Anzahl bei technisch realisierbaren Kuehlraten. Bei der Verfahrensoptimierung ist die genaue Abstimmung der Einflussparameter Zellart und -konzentration, Schutzadditivart und -zusammensetzung sowie Kuehl- und Erwaermungsrate zwingend erforderlich. (orig.)

  9. Fetal lung development on MRI. Normal course and impairment due to premature rupture of membranes; Fetale Lungenentwicklung in der MRT. Normaler Verlauf und Beeintraechtigung durch vorzeitigen Blasensprung

    Energy Technology Data Exchange (ETDEWEB)

    Kasprian, G. [Medizinische Universitaet Wien (Austria). Klinik fuer Radiodiagnostik; Zentrum fuer Anatomie und Zellbiologie der Medizinischen Universitaet Wien (Austria). Arbeitsgruppe Integrative Morphologie; Brugger, P.C. [Zentrum fuer Anatomie und Zellbiologie der Medizinischen Universitaet Wien (Austria). Arbeitsgruppe Integrative Morphologie; Helmer, H.; Langer, M. [Medizinische Universitaet Wien (Austria). Klinik fuer Frauenheilkunde; Balassy, C.; Prayer, D. [Medizinische Universitaet Wien (Austria). Klinik fuer Radiodiagnostik

    2006-02-15

    A well-organized interplay between many molecular factors as well as mechanical forces influence fetal lung development. At the end of this complex process a sufficiently sized and structurally mature organ should ensure the postnatal survival of the newborn. Besides prenatal ultrasonography, magnetic resonance imaging (MRI) can now be used to investigate normal and pathological human lung growth in utero. Oligohydramnios, due to premature rupture of membranes (PROM), is an important risk factor for compromised fetal lung growth. In these situations MR volumetry can be used to measure the size of the fetal lung quite accurately. Together with the evaluation of lung signal intensities on T2-weighted sequences, fetuses with pulmonary hypoplasia can be readily detected. (orig.) [German] Die fetale Lungenentwicklung wird einerseits durch eine Vielzahl molekularer Faktoren und andererseits durch mechanisch-physiologische Kraefte beeinflusst. Ein geordnetes Zusammenspiel dieser Mechanismen fuehrt zu einem ausreichend grossen und strukturell reifen Organ, das sofort nach der Geburt das Ueberleben des Neugeborenen sicherstellt. Neben der praenatalen Ultraschalluntersuchung bietet nun auch die Magnetresonanztomographie (MRT) die Moeglichkeit, die normale und pathologische fetale Lungenentwicklung zu untersuchen. Ein wesentlicher Risikofaktor fuer eine Beeintraechtigung der Lungenentwicklung ist die verminderte Fruchtwassermenge nach vorzeitigem Blasensprung. In diesen Faellen kann die MR-Volumetrie dazu eingesetzt werden, die Groesse der fetalen Lungen relativ genau zu bestimmen. Gemeinsam mit der Beurteilung der MR-Signalintensitaeten des Lungengewebes auf T2-gewichteten Sequenzen koennen Feten mit hypoplastischen Lungen mit zunehmender Sicherheit bereits praenatal identifiziert werden. (orig.)

  10. Properties of polymer-silver nanocomposites fabricated by co-sputtering; Eigenschaften von Polymer-Silber-Nanokompositen hergestellt durch Co-Sputtern

    Energy Technology Data Exchange (ETDEWEB)

    Schuermann, Ulrich

    2006-07-01

    In this thesis polymer-metal nanocomposites were fabricated by simultaneous sputtering of silver and polymers from two independent magnetron sputtering sources. By the netted structure and the dielectric properties PTFE is suited for the use as matrix material. The metal content of the composite films was determined via the measurement of EDX intensities. The size of the nanoparticles was determined by means of TEM and XRD and lies in the range of 10 nm. The composite materials show at the percolation limit a sudden transition of the properties from polymer- to metal-like behaviour. The specific resistance decreases in a range between 35 and 40 % silver content by at least nine orders of magnitude. The optical properties change with the metal content. The absorption caused by plasmon resonance, which has at small filling degrees its maximum at about 000 nm and has an intense yellow colour of the composite material as consequence shifts with increasing silver content to longer wavelengths. The difference in the optical.behaviour of the nanocomposites and the pure polymers can be used for the fabrication of Bragg reflectors. Small changes in the metal content can in the polymer-silver nanocomposites in the range of the percolation threshold effect a drastic change in ther properties, by which applications as sensors are thinkable. [German] In dieser Arbeit wurden Polymer-Metall-Nanokomposite durch gleichzeitiges Sputtern von Silber und Polymeren aus zwei unabhaengigen Magnetron-Sputterquellen hergestellt. Durch die vernetzte Struktur und die dielektrischen Eigenschaften eignet sich PTFE zur Verwendung als Matrixmaterial. Der Metallgehalt der Kompositfilme wurde ueber die Messung von EDX-Intensitaeten ermittelt. Die Groesse der Nanopartikel wurde mit Hilfe von TEM und XRD bestimmt und liegt im Bereich von 10 nm. Die Kompositmaterialien zeigen an der Perkolationsgrenze einen schlagartigen Uebergang der Eigenschaften vom polymer- zum metallaehnlichen Verhalten. Der

  11. Properties of polymer-silver nanocomposites fabricated by co-sputtering; Eigenschaften von Polymer-Silber-Nanokompositen hergestellt durch Co-Sputtern

    Energy Technology Data Exchange (ETDEWEB)

    Schuermann, Ulrich

    2006-07-01

    In this thesis polymer-metal nanocomposites were fabricated by simultaneous sputtering of silver and polymers from two independent magnetron sputtering sources. By the netted structure and the dielectric properties PTFE is suited for the use as matrix material. The metal content of the composite films was determined via the measurement of EDX intensities. The size of the nanoparticles was determined by means of TEM and XRD and lies in the range of 10 nm. The composite materials show at the percolation limit a sudden transition of the properties from polymer- to metal-like behaviour. The specific resistance decreases in a range between 35 and 40 % silver content by at least nine orders of magnitude. The optical properties change with the metal content. The absorption caused by plasmon resonance, which has at small filling degrees its maximum at about 000 nm and has an intense yellow colour of the composite material as consequence shifts with increasing silver content to longer wavelengths. The difference in the optical.behaviour of the nanocomposites and the pure polymers can be used for the fabrication of Bragg reflectors. Small changes in the metal content can in the polymer-silver nanocomposites in the range of the percolation threshold effect a drastic change in ther properties, by which applications as sensors are thinkable. [German] In dieser Arbeit wurden Polymer-Metall-Nanokomposite durch gleichzeitiges Sputtern von Silber und Polymeren aus zwei unabhaengigen Magnetron-Sputterquellen hergestellt. Durch die vernetzte Struktur und die dielektrischen Eigenschaften eignet sich PTFE zur Verwendung als Matrixmaterial. Der Metallgehalt der Kompositfilme wurde ueber die Messung von EDX-Intensitaeten ermittelt. Die Groesse der Nanopartikel wurde mit Hilfe von TEM und XRD bestimmt und liegt im Bereich von 10 nm. Die Kompositmaterialien zeigen an der Perkolationsgrenze einen schlagartigen Uebergang der Eigenschaften vom polymer- zum metallaehnlichen Verhalten. Der

  12. Unterstützung der IT-Service-Management-Prozesse an der Technischen Universität München durch eine Configuration-Management-Database

    Science.gov (United States)

    Knittl, Silvia

    Hochschulprozesse in Lehre und Verwaltung erfordern durch die steigende Integration und IT-Unterstützung ein sogenanntes Business Alignment der IT und damit auch ein professionelleres IT-Service-Management (ITSM). Die IT Infrastructure Library (ITIL) mit ihrer Beschreibung von in der Praxis bewährten Prozessen hat sich zum de-facto Standard im ITSM etabliert. Ein solcher Prozess ist das Konfigurationsmanagement. Es bildet die IT-Infrastruktur als Konfigurationselemente und deren Beziehungen in einem Werkzeug, genannt Configuration Management Database (CMDB), ab und unterstützt so das ITSM. Dieser Bericht beschreibt die Erfahrungen mit der prototypischen Einführung einer CMDB an der Technischen Universität München.

  13. Reducing available amounts of heavy metals in contaminated soils by adding iron oxides. Final report; Verringerung der Bioverfuegbarkeit von Schwermetallen in kontaminierten Boeden durch Zugabe von Eisenoxiden. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Pluquet, E.; Mueller, I.

    1998-02-01

    This project was part of a German-French research co-operation and investigated the practicability to immobilize heavy metals by adding iron oxides to contaminated soils (harbour sediment, bracky marsh contaminated by emission of a zinc/lead smelter, alluvial Gley soil contaminated by mining activity). Pot and field trials were carried out using three different soils. Five iron bearing materials were added to the soil (1% pure Fe in soil dry matter): Red mud from Al-industry, Fe-precipitation sludge from drinking water treatment, bog iron ore, unused steel shot and steel shot waste from surface treatment of rolled steel plates. When mixed with the soil NH{sub 4}NO{sub 3} and DTPA extractable amounts of heavy metals and uptake by plants was markedly reduced. It was demonstrated that precipitated Fe-bearing sludge from drinking water treatment was a very effective material reducing the NH{sub 4}NO{sub 3} soluble amounts by 40-50% (DTPA 30-40%) in the pot tests while the uptake by plants was reduced by 40% and more. Heavy metals were strongly bound in a fixed order Cd>Pb>Zn>Cu and stayed immobilized until the end of project. Heavy metals were immobilized to a certain extent in field trials, but less effect was observed on concentration in plants and soil extracts compared with the pot tests. In practice the treatment should exceed the 1% pure Fe tested and the application should more homogeneously affect the soil layer where the plant roots mainly grow. (orig.) [Deutsch] Im Rahmen eines deutsch-franzoesischen Projektverbundes wurde die Moeglichkeit der Immobilisierung von Schwermetallen in kontaminierten Boeden durch gezielte Zufuhr von Eisenoxiden untersucht. Die praxisorientierten Untersuchungen wurden in Gefaess- und Feldversuchen mit drei unterschiedlichen Boeden (Spuelfeldboden aus Hafenschlick, immissionsbelastete Brackmarsch, durch Bergbau belasteter Auenboden) ausgefuehrt. Dazu wurden fuenf eisen(oxid)haltige Materialien (Rotschlamm aus der Al

  14. BioCrack {sup registered}. Enhanced gas yield by electrokinetic disintegration; BioCrack {sup registered}. Steigerung der Gasausbeute durch elektrokinetische Desintegration

    Energy Technology Data Exchange (ETDEWEB)

    Liebich, Markus [Hugo Vogelsang Maschinenbau GmbH (Germany)

    2011-07-01

    In the electrokinetic disintegration process, the fluid flows through a pipeline system to which a HV field is applied. The electric forces of the HV field deform and destabilise the cell membrane. Once the deformation exceeds the elastic resistance of the cell membrane, the cell membrane will become permeable, and the cell content will be released and can be used for gas production. The advantages of theprocess are the low investment cost, simple retrofitting, high availability, and low energy cost (35 W per module). [German] Bei der elektrokinetischen Desintegration durchfliesst das Medium ein Rohrleitungssystem in dem ein Hochspannungsfeld anliegt. Durch die auftretenden elektrischen Kraefte im Hochspannungsfeld wird die Zellmembran verformt und destabilisiert. Uebersteigt die Verformung die elastische Widerstandkraft der Zellmembran, so wird diese durchlaessig. Dadurch werden die Zellinhaltsstoffe freigesetzt und koennen zur Gaserzeugung genutzt werden. Die Vorteile dieses Verfahrens liegen in den geringen Investitionskosten gegenueber anderen Desintegrationsverfahren, der einfachen Nachruestbarkeit, der hoher Anlagenverfuegbarkeit sowie in den geringen Energiekosten (35 W pro Modul).

  15. Improved energetic balance of supermarket refrigerators by efficient working fluids and new technology; Verbesserung der energetischen Gesamtbilanz von Supermarkt-Kaelteeinrichtungen durch die Verwendung eines effizienten Kaeltetraegers und neuer Technik

    Energy Technology Data Exchange (ETDEWEB)

    Svensson, K. [Frigotech AB, Stockholm (Sweden); Hellsten, J. [Aspen Petroleum AB, Hindaas (Sweden)

    2000-07-01

    Secondary refrigeration systems were first developed in Sweden. Aspen Petroleum, a Swedish company which uses exclusively environment-friendly products, developed a new, non-polluting and highly efficient refrigerant, Aspen Temper, which is biodegradable and made of renewable materials. Aspen Temper comprises a patented, highly effective and reliable corrosion protection technology. This contribution describes a new cooperative project of Aspen Petroleum and Frigotech, Stockholm, in the field of supermarket refrigeration systems. [German] In Schweden gibt es eine laengere und gute Erfahrung mit Sekundaerkaelteanlagen im Bereich Supermarktkaelte. Die Technik der Sekundaerkaelteanlagen wurde in Schweden entwickelt und entspricht die Erwartungen, nicht nur ein umweltschonendes Konzept fuer den Supermarkt zu sein, sondern auch den Energieverbrauch zu reduzieren und gleichzeitig die Produktqualitaet des Lebensmittels zu erhoehen. Durch den schnellen Ausstieg aus den FCKW's und die Begrenzung der maximalen Fuellmenge mit Kaeltemittel, hat der Markt fuer Sekundaerkaelte im Supermarktbereich frueh in Schweden begonnen. Aspen Petroleum, ein Schwedisches Unternehmen das nur mit umweltschonenden Produkten arbeitet, hat einen neuartigen, patentierten, umweltschonenden und sehr effizienten Kaelte- und Waermetraeger entwickelt und ist seit ein paar Jahren damit auf dem Markt. Der Name dieser Fluessigkeit ist Aspen Temper, die biologisch abbaubar ist und aus regenerativen Rohstoffen hergestellt wird. Im Vergleich mit anderen, aehnlichen Fluessigkeiten auf dem Markt, enthaelt Aspen Temper eine sehr effektive und zuverlaessige Korrosionsschutztechnologie, die auch patentiert ist. Durch eine Zusammenarbeit der Firma Aspen und der Installateurfirma Frigotech in Stockholm, die ausserdem Kaeltemaschinen in Modulbauweise liefert, ist ein sehr interessantes Konzept entstanden, das in diesem Vortrag, inklusive interessanter Referenzanlagen in Supermaerkten praesentiert wird. Diese

  16. Rhodopseudomonas acidophila strain 10050 contains photosynthetic LH2 antenna complexes that are not enriched with phosphatidylglycerol, and the phospholipids have a fatty acyl composition that is unusual for purple non-sulfur bacteria.

    Science.gov (United States)

    Russell, Nicholas J; Coleman, Julie K; Howard, Tina D; Johnston, Evelyn; Cogdell, Richard J

    2002-12-01

    The phospholipid composition of Rhodopseudomonas acidophila strain 10050 grown aerobically or anaerobically in the light was determined. The major phospholipids present in the aerobic cells were phosphatidylethanolamine (PE; 54%), phosphatidylglycerol (PG; 24%) and cardiolipin (diphosphatidylglycerol, DPG) (14%), together with phosphatidylcholine (PC; 5%). On moving the cells to anaerobic photosynthetic growth in the light PE remained the major phospholipid (37-49%), but there was a major change in the proportion of PC, which increased to 31-33%, and corresponding reductions in the contents of PG to 11-16% and DPG to 4-5%. The fatty acid composition of the phospholipids was unusual, compared with other purple non-sulfur photosynthetic bacteria, in that it contained 16:0 (29%), 17:1 (20%) and 19:1 (9%) plus several mainly unsaturated 2-OH fatty acids (9% total) as major components, when grown aerobically in the dark. In contrast when grown photosynthetically under anaerobic conditions there was <2% 17:1 or 19:1 present, while the amounts of 16:1 and 18:1 increased, and 16:0 decreased. The phospholipid composition of the purified light-harvesting complex 2 (LH2) complex was PE (43%), PC (42%) and DPG (15%). Unexpectedly, there was no PG associated with the purified LH2. These findings contrast with previous studies on several other photosynthetic bacteria, which had shown an increase in PG upon photosynthetic growth [Biochem. J. 181 (1979) 339]. The prior hypothesis that phosphatidylglycerol has some specific role to play in the function of light-harvesting complexes cannot be true for Rps. acidophila. It is suggested that specific integral membrane proteins may strongly influence the phospholipid content of the host membranes into which they are inserted.

  17. MR imaging after osteotomy of the proximal tibia with high-grade steel staples; MR-Bildgebung nach Tibiakopf-Umstellungsosteosynthese mit Fixierung durch Edelstahl-Klammern

    Energy Technology Data Exchange (ETDEWEB)

    Schick, F. [Abt. fuer Radiologische Diagnostik, Tuebingen Univ. (Germany)]|[Physikalisches Inst., Tuebingen Univ. (Germany); Duda, S. [Abt. fuer Radiologische Diagnostik, Tuebingen Univ. (Germany); Heinrich, F.E. [Abt. fuer Allgemeine Orthopaedie, Tuebingen Univ. (Germany)

    1993-12-31

    Metallic implants may change static as well as radio frequency magnetic fields in the surrounding tissues during MR imaging. The influence of staples made of high-grade steel on MR spin-echo, inversion recovery spin-echo, and gradient-echo imaging techniques was evaluated. The healing in 6 patients after osteotomy of the proximal tibia was investigated. The artifacts in spin-echo techniques are influenced by the amplitudes of the static field gradients. The applied echo times and repetition times are irrelevant. The artifacts which result from the staples are linear structures with high signal superimposed to the regular signals in the images. In contrast gradient-echo techniques show a loss of signal near the staples depending on the echo time used. The inversion recovery method allows to determine the distribution of the water protons in the surroundings of the osteotomy. Sequential MR imaging is suitable to non-invasively examine the healing process after osteotomy. Some special aspects of MR techniques in the presence of metallic foreign bodies must be considered. (orig.) [Deutsch] Der Einfluss von Edelstahl-Fixationsklammern, die durch ihre magnetischen Eigenschaften sowohl das statische als auch das Hochfrequenzfeld bei der MR-Tomographie beeinflussen, wird fuer Spinecho-, Inversion-Recovery-Spinecho- und Gradientenecho-Verfahren untersucht. Dabei konnte der Heilungsverlauf bei 6 Patienten nach Tibiakopf-Osteosynthese verfolgt werden. Es wird gezeigt, dass bei Spinecho-Verfahren die verwendeten Feldgradientenamplituden die Artefakte beeinflussen, waehrend die gewaehlte Echozeit und Repetitionszeit unerheblich sind. Die Artefakte zeichnen sich durch linienfoermige Hyperintensitaeten aus, die richtig ortskodierten Strukturen ueberlagert sind. Dagegen ergaben Gradientenecho-Verfahren echozeitabhaengige Signalausloeschungen im Bereich der Fixationsklammern. Das Inversion-Recovery-Verfahren erlaubt eine selektive Beurteilung der Wassersignale im Osteotomiebereich

  18. Light-Driven Amino Acid Uptake in Streptococcus cremoris or Clostridium acetobutylicum Membrane Vesicles Fused with Liposomes Containing Bacterial Reaction Centers

    NARCIS (Netherlands)

    Crielaard, Wim; Driessen, Arnold J.M.; Molenaar, Douwe; Hellingwerf, K; Konings, Wilhelmus

    1988-01-01

    Reaction centers of the phototrophic bacterium Rhodopseudomonas palustris were introduced as proton motive force-generating systems in membrane vesicles of two anaerobic bacteria. Liposomes containing reaction center-light-harvesting complex I pigment protein complexes were fused with membrane vesic

  19. A comparison of the unfolding and dissociation of the large ribosome subunits from Rhodopseudomonas spheroides N.C.I.B. 8253 and Escherichia coli M.R.E. 600.

    Science.gov (United States)

    Robinson, A; Sykes, J

    1973-08-01

    1. The behaviour of the large ribosomal subunit from Rhodopseudomonas spheroides (45S) has been compared with the 50S ribosome from Escherichia coli M.R.E. 600 (and E. coli M.R.E. 162) during unfolding by removal of Mg(2+) and detachment of ribosomal proteins by high univalent cation concentrations. The extent to which these processes are reversible with these ribosomes has also been examined. 2. The R. spheroides 45S ribosome unfolds relatively slowly but then gives rise directly to two ribonucleoprotein particles (16.6S and 13.7S); the former contains the intact primary structure of the 16.25S rRNA species and the latter the 15.00S rRNA species of the original ribosome. No detectable protein loss occurs during unfolding. The E. coli ribosome unfolds via a series of discrete intermediates to a single, unfolded ribonucleoprotein unit (19.1S) containing the 23S rRNA and all the protein of the original ribosome. 3. The two unfolded R. spheroides ribonucleoproteins did not recombine when the original conditions were restored but each simply assumed a more compact configuration. Similar treatments reversed the unfolding of the E. coli 50S ribosomes; replacement of Mg(2+) caused the refolding of the initial products of unfolding and in the presence of Ni(2+) the completely unfolded species (19.1S) again sedimented at the same rate as the original ribosomes (44S). 4. Ribosomal proteins (25%) were dissociated from R. spheroides 45S ribosomes by dialysis against a solution with a Na(+)/Mg(2+) ratio of 250:1. During this process two core particles were formed (21.2S and 14.2S) and the primary structures of the two original rRNA species were conserved. This dissociation was not reversed. With E. coli 50S approximately 15% of the original ribosomal protein was dissociated, a single 37.6S core particle was formed, the 23S rRNA remained intact and the ribosomal proteins would reassociate with the core particle to give a 50S ribosome. 5. The ribonuclease activities in R

  20. Energy saving by enhanced temperature measurement of stock materials of heat- and heat treatment process. Final report; Energieeinsparung durch verbesserte Nutzguttemperaturbestimmung bei Waerm- und Waermebehandlungsprozessen. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Ley, I.; Klima, R.

    1999-06-01

    Thermoelementes, welches moeglichst gut im Strahlungsaustausch mit dem Nutzgut steht, gemessen. Somit kann die Oberflaechentemperatur weitgehend unabhaengig vom Emissionsgrad des Nutzgutes gemessen werden. Die Vorteile der Thermoelementsonde konnten durch eine Installation an einem Waermeofen und einer Waermebehandlungsanlage deutlich aufgezeigt werden. Durch die dauerhafte betriebliche Installation konnten umfangreiche Verbesserungsmassnahmen zur Energieeinsparung und zur Qualitaetsverbesserung an den Oefen realisiert werden. Die Thermoelementsonde ermoeglicht eine strenge Optimierung mittels modellgestuetzter Steuerung und damit deutliche Produktverbesserungen und Energieeinsparungen. (orig.)

  1. Die Analyse der Inhibition des Monozyten chemotaktischen Proteins-1 (MCP-1) und der Stimulation durch MCP-1 auf die Koloniebildung und die Zytokinexpression von Plattenepithelkarzinomen der Kopf-Hals-Region im FLAVINO-Assay

    OpenAIRE

    Körner, Carolin

    2015-01-01

    Das Monozyten chemotaktische Protein-1 (MCP-1) ist ein CC-Chemokin, das in seiner Rolle als Chemoattraktor auf Monozyten in der Genese von Malignomen eine wesentliche Rolle spielt. Dabei kann es sowohl zur lokalen Tumorabwehr als auch zur Tumorgenese, Tumor-angiogenese und Metastasierung beitragen. Die vorliegende Arbeit untersucht die MCP-1-Inhibition und die Stimulation durch MCP-1 auf die Koloniebildung und die Zytokinexpression von Plattenepithelkarzinomen der Kopf-Hals-Region (HNSCC) im ...

  2. 孔雀鱼体表变形虫病及其防治研究%Prevention and Treatment of the Disease Caused by Pelomyxa palustris Greeff in Guppy(Poecilia reticulate)

    Institute of Scientific and Technical Information of China (English)

    李赫; 李文宽; 胡宗云; 富丽静; 宋文华; 王庆海; 张涛; 张健

    2016-01-01

    2014年10-11月,辽宁某地温室大棚观赏鱼场养殖的孔雀鱼(Poecilia reticulate)普遍暴发了体表变形虫病,其病原初步鉴定为池沼多核变形虫 Pelomyxa palustris Greeff。病鱼的主要症状是游动缓慢,食欲减退,以至不摄食;体表两侧局部溃烂,镜检病灶处可见大量虫体。防治试验结果表明:乙酸和过氧化氢混合使用(1∶3),浓度为0.5%~1.0%,浸泡4~5 min ,有一定杀虫效果。盐酸奎宁浓度为500~1500 mg/L ,浸泡15 min ,有一定杀虫效果。青蒿素+ T M P+高甲醚(300 mg/L∶60 mg/L∶10 mg/L )浸泡20~30 min ,杀虫效果较好。%From October to November in 2014 ,Guppy cultivated in greenhouse of ornamental fish farm in Liaoyang area commonly broke out amoebiasis .The pathogen was preliminarily identified as Pelo‐myxa palustris .Principal apparent symptoms of diseased fish were swimming slowly ,decreased ap‐petitie ,up to no feeding and fester on partial surface of both sides .A large number of P .p alustris were found in microscopic examination .Experiment results showed that both soaking the diseased fish in 0.5% ~1 .0% mixed liquid of acetic acid and hydrogen peroxide (1:3) for 4~5 min and in 500~1 500 mg/L of Quinine hydrochloride for 15 min had some effect .It had better control effect by soaking the diseased fish in mixed liquid of artemisinin ,TMP and artemether(300 mg/L∶60 mg/L∶10 mg/L)for 20~30 min .

  3. THERMOSELECT. Continuous environment-friendly treatment of residues by gasification and direct melting; THERMOSELECT. Unterbrechungslose umweltgerechte Restabfallbehandlung durch Vergasung und Direkteinschmelzung

    Energy Technology Data Exchange (ETDEWEB)

    Gaeng, P.; Klein, K.; Stahlberg, R.; Weisenburger, P. [Thermoselect Suedwest GmbH, Karlsruhe (Germany)

    1996-12-31

    The Thermoselect process comprises waste compaction, degassing, gasification with pure oxygen, and melting of inorganic components in a continuous closed cycle process. The high-quality products can be recycled almost completely: The synthesis gas can be used as feedstock or combusted, also in high-efficiency power generation processes. The other products, e.g. the high-grade mineral and metal granulates, can be utilized without restrictions. The residual fraction which must be dumped is very small. (orig) [Deutsch] Mit Thermoselect ist eine neue Technik auf dem Markt, die durch die konsequente Umsetzung der Verfahrensschritte Abfallverdichtung, Entgasung, Vergasung mit reinem Sauerstoff und Einschmelzung der anorganischen Muellbestandteile in einem unterbrechungslosen geschlossenen Prozess hochwertige Produkte aus Abfaellen erzeugt, die nahezu vollstaendig verwertet werden koennen. Das erzeugte Synthesegas kann sowohl stofflich als auch thermisch genutzt werden, wobei der Einsatz von stromerzeugungsverfahren mit hohen Wirkungsgraden moeglich ist. Die ubrigen Produkte, z.B. das mineralische und das metallische Granulat, erreichen eine Qualitaet, die eine uneingeschraenkte Nutzung zulaesst. Die Anteile der z.Zt. noch zu entsorgenden Reststoffe ist gering, wobei derzeit meist wirtschafatliche Gruende deren Entsorgung nahelegen. (orig)

  4. Use of dexpanthenol and aloe vera to influence the irradiation response of the oral mucous membrane (mouse); Beeinflussung der Strahlenreaktion der Mundschleimhaut (Maus) durch Dexpanthenol {+-} Aloe vera

    Energy Technology Data Exchange (ETDEWEB)

    Schlichting, S. [Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie, Dresden Univ. (Germany); Spekl, K.; Doerr, W. [Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie, Dresden Univ. (Germany)]|[Experimentelles Zentrum, Medizinische Fakultaet Carl Gustav Carus, Dresden Univ. (Germany)

    2004-07-01

    In summarising the outcome of the present study it can be said spraying the agent on the oral mucous membrane once a day had an effect on the incidence of mucous membrane ulceration in the case of both placebo and dexpanthenol treatment. However, there was no significant difference between placebo and dexpanthenol treatment, the only finding being a slight prolongation of latency time through aloe vera. These experimental findings give good reason to critically reconsider the clinical use of dexpanthenol as a supportive treatment for the prevention of radiogenic mucositis enoralis following irradiation of tumours in the head and neck region. However thorough oral lavage is an effective means of moderating the irradiation response of the oral mucous membrane. [German] Zusammenfassend ist festzustellen, dass in den vorliegenden Versuchen mit einmal taeglichem Aufspruehen des Praeparates auf die Mundschleimhaut sowohl die Placebo - wie auch die Dexpanthenol-Behandlung die Inzidenz von Schleimhautulzerationen modifiziert hat; zwischen Placebo- und Dexpanthenol-Behandlung ergab sich jedoch kein signifikanter Unterschied. Lediglich eine geringe Verlaengerung der Latenzzeit durch Aloe vera war zu beobachten. Auf der Basis dieser experimentellen Ergebnisse muss der klinische Einsatz von Dexpanthenol im Rahmen der Supportivtherapie zur Prophylaxe der radiogenen Mucositis enoralis bei der Bestrahlung von Kopf-Hals-Tumoren kritisch ueberdacht werden. Regelmaessige, intensive Mundspuelungen sind jedoch ein probates Mittel zur Verminderung der Strahlenreaktion der Mundschleimhaut. (orig.)

  5. Reactor for biological elimination of poorly degradable hydrocarbons by adjustment of substrate-specific retention times; Reaktor zur biologischen Eliminierung schwer abbaubarer Kohlenwasserstoffe durch Einstellung substratspezifischer Verweilzeiten

    Energy Technology Data Exchange (ETDEWEB)

    Brambach, R.

    1997-11-01

    Industrial waste water cleaning increasingly makes use of biological processes based on immobilized biomass, in order to ensure the aimed elimination of toxic substances. A novel, efficient reactor technique was developed, by which dangerous, poorly degradable dissolved hydrocarbons are selectively retained in the bioreactor thanks to an integrated membrane, while other substances are quickly discharged from the system via the effluent. With a synthetic waste water, this selective retention method achieved substrate-specific retention times which were five to fifty times higher than those of a merely hydrodynamically operated reactor system. In addition, the decoupling of high reactor and low effluent concentrations results in a higher biochemical activity in the reactor in comparison with conventional process techniques. (orig.) [Deutsch] In der industriellen Abwasserreinigung werden verstaerkt Bioverfahrenstechniken mit immobilisierter Biomasse eingesetzt, um eine gezielte Eliminierung gefaehrlicher Stoffe durchzufuehren. - Hierzu wurde eine neuartige leistungsfaehige Reaktortechnik entwickelt, die mittels einer im Bioreaktor integrierten Membran gefaehrliche, schwer abbaubare geloeste Kohlenwasserstoffe selektiv im Bioreaktor zurueckhaelt, waehrend andere Stoffe das System schnell ueber den Ablauf verlassen. Durch den stoffselektiven Rueckhalt wurden an einem synthetischen Abwasser substratspezifische Verweilzeiten erzielt, die um den Faktor 5 bis 50 ueber denen eines rein hydrodynamisch bestimmten Reaktorsystems liegen. Darueber hinaus wird aufgrund der Entkopplung von hoher Reaktor- und niedriger Ablaufkonzentration eine im Vergleich zu herkoemmlichen Verfahrenstechniken hoehere biochemische Aktivitaet im Reaktor erzielt. (orig.)

  6. CO{sub 2} reduction potential of the use of plastic wastes as feedstock in various energy conversion processes; CO{sub 2}-Reduktionspotential durch den Einsatz von Plastikabfaellen in verschiedenen Energiewandlungsprozessen

    Energy Technology Data Exchange (ETDEWEB)

    Romey, I.; Obladen, M. [Essen Univ. (Germany)

    2000-07-01

    Plastic wastes are available in large quantities in Germany (almost 800,000 t per year) and their reutilisation is compulsory under the Recycling Economy Law. This may either be done by raw material recycling or by energy conversion processes. The use of plastic wastes characterised by a high calorific value and low specific CO{sub 2} emissions constitutes a high potential for reducing CO{sub 2} emissions. The CO{sub 2} emissions caused by the additional energy input for preparing and pretreating the plastic are in most cases more than compensated for by the favourable H/C ratio. This paper compares altogether six energy conversion processes in terms of their CO{sub 2} balance. Each individual process is compared with and assessed on the basis of a reference scenario, in accordance with the method of Life Cycle Analysis (LCA). It transpires that in general none of the three process variants materials recycling, raw material recycling, and energy recovery is superior to any other in terms of the CO{sub 2} balance. With the more favourable processes the use of plastic wastes results in a reduction of up to 10% in CO{sub 2} emissions. [German] Plastikabfaelle sind in Deutschland in einer grossen Menge (knapp 800.000 t pro anno) verfuegbar und muessen gemaess Kreislaufwirtschaftsgesetz wiederverwertet werden. Dies ist sowohl durch Rohstoffrecycling als auch in Energiewandlungsprozessen moeglich. Durch den Einsatz von Plastikabfall mit einem charakteristisch hohen Brennwert und niedrigen spezifischen CO{sub 2}-Emissionen ergibt sich ein hohes Potential zur Reduzierung der CO{sub 2}-Emissionen. Die CO{sub 2}-Emissionen bedingt durch den Mehraufwand an Energie fuer die Aufbereitung und die Vorbehandlung des Plastik werden in der Regel durch den Einsatz des Brennstoffs mit dem guenstigeren H/C-Verhaeltnis ueberkompensiert. Im einzelnen werden sechs Energiewandlungsprozesse dargestellt und unter dem Aspekt der CO{sub 2}-Bilanz miteinander verglichen. Dabei wird gemaess der

  7. Substitution of wastes for fuels and raw materials in high-temperature processes; Substitution von Brennstoffen und Rohstoffen durch Abfaelle in Hochtemperaturprozessen

    Energy Technology Data Exchange (ETDEWEB)

    Scholz, R. [Technische Univ. Clausthal, Clausthal-Zellerfeld (Germany). Inst. fuer Energieverfahrenstechnik; Beckmann, M. [Clausthaler Umwelttechnik-Institut GmbH (CUTEC), Clausthal-Zellerfeld (Germany)

    1998-09-01

    burning processes in the cement industry. To do justice to the steel and iron industry it also deals with fuel substitution in a natural gas fired cokeless cupola furnace. [Deutsch] Die energetische und die stoffliche Verwertung von Abfaellen ist ein bereits seit langem diskutiertes Thema. Dabei interessieren zunaechst die Fragestellungen im Zusammenhang mit dem jeweils spezifischen Einsatz wie z.B. bei der Co-Verbrennung von Klaerschlamm in Kraftwerksfeuerungen, der Substitution von Primaerbrennstoffen durch Kunststoffabfaelle beim Brennprozess in der Zementindustrie usw. Darueber hinaus wird weiter der Vergleich der jeweiligen Anwendung untereinander sowie die Gegenueberstellung mit den zum Stand der Technik gehoerenden thermischen Abfallbehandlungsverfahren diskutiert. Diese Verfahrensvergleiche muessen selbstverstaendlich unter Einbeziehung aller insgesamt erforderlichen Aufwendungen an Zusatzstoffen und Zusatzenergie mit gleichen Randbedingungen durchgefuehrt werden. Dabei sind u.a. die sich aus den spezifischen Eigenschaften der Abfallstoffe fuer den jeweiligen Anwendungsfall ergebenden Besonderheiten zu beruecksichtigen. Das betrifft insbesondere die Auswirkungen einer Substitution von Primaerbrennstoffen durch Ersatzbrennstoffe aus Abfaellen (Sekundaerbrennstoffe) z.B. bei einem Brennprozess auf die Waermeuebertragungsbedingungen, die Stroemungsverhaeltnisse und damit zusammenhaengend auf die Temperaturverteilungen, den Guttransport und den spezifischen Energieaufwand. Ersatzbrennstoffe muessen sich sowohl aus stofflicher Sicht als auch aus brennstoff- und waermetechnischer Sicht fuer eine Substitution eignen. In dem vorliegenden Beitrag wird insbesondere auf die Anforderungen an Abfaellen, die zur Substitution von Primaerbrennstoffen dienen aus brennstoff- und waermetechnischer Sicht eingegangen. Hierzu werden zunaechst kurz einige wesentliche Gesichtspunkte der Waermeuebertragung in Feuerungen und Industrieoefen eroertert. Wichtig fuer die Bewertung einer

  8. Degradation of organic pollutants in sewage sludge by aerobic-thermophilic sludge treatment. Final report; Abbau organischer Schadstoffe im Klaerschlamm durch aerob-thermophile Schlammbehandlung. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Prechtl, S.

    1999-07-01

    Klaerschlamm. Im organischen Anteil des Klaerschlammfeststoffes sind bis heute ueber 300 verschiedene organische Spurenstoffe nachgewiesen worden. Neben den in der Klaerschlammverordnung erfassten Stoffgruppen (Dioxine/Furane und PCB) sind weitere Stoffgruppen als relevant eingestuft worden. Um die in diesem Bereich z.T. noch unzureichende Datenlage zu erweitern wurde im Forschungsvorhaben die Moeglichkeit untersucht, durch eine aerob-thermophile Schlammbehandlung (ATS) eine Verbesserung der Klaerschlammqualitaet, bei der Stoffgruppe der Phthalate (Di-(2-ethylhexyl)phthalat), den polyzklischen aromatischen Kohlenwasserstoffen (PAK) und beim 4-Nonylphenol (Abbauprodukt von nichtionischen Tensiden), zu erreichen. Zur Beurteilung der Abbaueffektivitaet aus der komplexen Matrix Klaerschlamm bildete die Schadstoffanalytik mittels HPLC und GC/MS einen Schwerpunkt des Vorhabens. Der Gehalt an Di-(2-ethylhexyl)phthalat (DEHP) und 4-Nonylphenol (4-NP) konnte in Laborversuchen mit dotierten Schlaemmen um 70% bzw. 50% und in undotierten Schlaemmen um 61% bzw. 53% reduziert werden. Bei halbtechnischen Versuchen lag die Reduktion fuer DEHP bei 14% und fuer 4-NP bei 68%. Fuer Pyren liess sich in Laborversuchen ein Abbau bis zu 57% erreichen, im Vergleich zu halbtechnischen Untersuchungen mit 22% Abbau. Die Ergebnisse erster grundlegender Untersuchungen zu einer Verfahrenskombination aus verkuerzter Faulstufe und ATS-Folgestufe zeigten fuer DEHP eine Reduktion von ca. 60%. Der Gehalt an einzelnen PAK konnten bis Anthracen reduziert werden. Fuer hoeherkondensierte PAK (Grundbelastung im ppb-Bereich) ergab sich keine einheitliche Aussage. Ein Abbau von 4-NP wurde bei den Versuchen zur Verfahrenskombination durch die Neubildung von 4-NP aus Alkylphenolethoxylaten unter anaeroben und aeroben Bedingungen ueberdeckt. Die erhaltenen Untersuchungsergebnisse bestaetigen die Korrelation zwischen einer Hygienisierung des Klaerschlamms und der entsprechenden Verweilzeit im Reaktorsystem. Sowohl in der

  9. Drag-out of stainless-steel alloying constituents through condensates which collect in high-efficiency boilers; Austrag von Legierungsbestandteilen aus nichtrostenden Staehlen durch Kondensate in Brennwertanlagen

    Energy Technology Data Exchange (ETDEWEB)

    Arlt, N. [Thyssen Stahl AG, Werk Krefeld (Germany). Werkstofftechnik Rostfrei; Schlerkmann, H. [Mannesmannroehren-Werke AG, Duisburg (Germany). Korrosionschemie; Stellfeld, I. [Krupp Hoesch Stahl AG, Werksgruppe Duesseldorf (Germany). Metallurgische Technologie; Steinbeck, G. [Verein Deutscher Eisenhuettenleute (VDEh), Duesseldorf (Germany). Fachbereich Werkstofftechnik

    1994-11-07

    Heating systems which are equipped with high- efficiency boilers save energy because the energy content of the flue gases is utilized through flue gas condensation. Stainless steel is an important high-efficiency boiler material. The chromium and nickel concentrations of acid condensates from high- efficiency systems in waste water were analyzed. Stainless-steel samples were immersed in a model condensate over a period of 10 weeks. Fresh model condensates were prepared after one week and three weeks to measure metal dissolution as a function of time. The longer the samples were immersed the more did the nickel concentrations in the model concentrate decrease. Without exception, the concentrations were clearly below the maximum permissible values laid down in leaflet M 251 which is issued by Abwassertechnische Vereinigung e.V. The expected inadmissibly high nickel concentrations did not occur in the acid condensates. (orig.) [Deutsch] Heizungsanlagen mit Brennwertkesseln arbeiten energiesparend, da durch eine Abgaskondensation der Energieinhalt der Abgase nutzbar gemacht wird. Ein wichtiger Konstruktionswerkstoff fuer Brennwertanlagen ist nichtrostender Stahl. Untersucht werden sollte die Frage, ob die sauren Kondensate der Brennwertanlagen, die ins Abwasser abgefuehrt werden unzulaessig hohe Chrom- und Nickelkonzentrationen aufweisen koennen. Zu diesem Zweck wurden Proben aus nichtrostenden Staehlen ueber einen Gesamtzeitraum von 10 Wochen in einem Modellkondensat ausgelagert. Dabei wurde das Modellkondensat nach einer Woche und nach drei Wochen Auslagerungzeit frisch angesetzt, um die zeitliche Abhaengigkeit der Metallaufloesung zu erfassen. Es wurde festgestellt, dass die Nickelkonzentrationen im Modellkondensat mit zunehmender Auslagerungsdauer drastisch abnehmen; sie lagen in allen Faellen deutlich unter den zulaessigen Richtwerten des Merkblattes M 251 der Abwassertechnischen Vereinigung e.V. Die Vermutung, dass unzulaessig hohe Konzentrationen an Nickel in

  10. Epiphyseal injuries of the distal tibia. Does MRI provide useful additional information?; Epiphysenfugenverletzungen der distalen Tibia. Sinnvolle Mehrinformation durch die MRT?

    Energy Technology Data Exchange (ETDEWEB)

    Iwinska-Zelder, J.; Schmidt, S.; Ishaque, N.; Klose, K.J.; Hoppe, M. [Marburg Univ. (Germany). Abt. fuer Strahlendiagnostik; Schmitt, J.; Gotzen, L. [Marburg Univ. (Germany). Medizinisches Zentrum fuer Operative Medizin

    1999-01-01

    Plain film radiography often underestimates the extent of injury in children with epiphyseal fracture. Especially Salter-Harris V fractures (crush fracture of the epiphyseal plate) are often primarily not detected. MRI of the ankle was performed in 10 children aged 9-17 (mean 14) years with suspected epiphyseal injury using 1.0-T Magnetom Expert. The fractures were classified according to the Salter-Harris-Rang-Odgen classification and compared with the results of plain radiography. In one case MRI could exclude epiphyseal injury; in four cases the MRI findings changed the therapeutic management. The visualisation of the fracture in three orthogonal planes and the possibility of detection of cartilage and ligamentous injury in MR imaging makes this method superior to conventional radiography and CT. With respect to radiation exposure MRI instead of CT should be used for the diagnosis of epiphyseal injuries in children. (orig.) [Deutsch] Die konventionelle Roentgendiagnostik unterschaetzt haeufig das Ausmass der kindlichen Extremitaetenfrakturen mit Epiphysenbeteiligung (Typ Salter-Harris). Insbesondere werden die Kompressionsfrakturen der Wachstumsfuge (Salter-Harris V) primaer haeufig nicht erkannt. Prospektiv wurden 10 Kinder im Alter von 9-17 Jahren (Durchschnittsalter = 14 J.) mit Verdacht auf eine epiphysaere Fraktur der distalen Tibia magnetresonanztomographisch (1.0-Tesla Magnetom Expert), untersucht. Die MRT-Ergebnisse wurden auf der Basis der Klassifikation nach Salter-Harris-Rang-Odgen mit den konventionellen Roentgenbildern verglichen. In einem Fall, bei einem 15jaehrigen Patienten, gelang durch die MRT der Ausschluss einer epiphysaeren Verletzung. In 7 Faellen fuehrte der MRT-Einsatz zu einer Aenderung der Klassifikation nach Salter-Harris. Hieraus resultierte bei 4 Patienten ein Therapiewechsel. Da Frakturen, die sie begleitenden Knorpellaesionen und ligamentaere Verletzungen multiplanar dargestellt werden koennen, weist die MRT deutliche Vorteile

  11. Differentiation of impregnation by non-impregnation of high volatile coal seams; Differenzierung der Anwendung der Traenkarbeit durch Traenkverzicht in fuer die Traenkung ungeeigneten Floezpartien

    Energy Technology Data Exchange (ETDEWEB)

    Henke, B. [Deutsche Montan Technologie GmbH, Essen (Germany). Gas and Fire Div.

    2004-07-01

    Feststellung der Eignung einer Kohlenprobe fuer den Traenkverzicht eroeffnet die Moeglichkeit, durch selektive Betrachtung einzelner Floeze bis hin zu einzelnen Abbaubetrieben den Traenkverzicht auszuweiten. (orig.)

  12. Study on the planning of a demonstration plant for hydrogen fuel production by electrolysis using caching in salt caverns under pressure; Studie ueber die Planung einer Demonstrationsanlage zur Wasserstoff-Kraftstoffgewinnung durch Elektrolyse mit Zwischenspeicherung in Salzkavernen unter Druck

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-02-05

    In a two year research project the long-term storage of hydrogen as energy carrier has been assessed in detail for large renewable electricity quantities in underground storage caverns in Germany. Next to a regions analysis for potential storage sites, the benchmarking against other large scale storage concepts, a comparative and perspective analysis of alkaline and PEM-electrolysers also potential business cases for Power-to-Hydrogen in the mobility sector as well as for the chemical, electricity and natural gas industry have been analysed. [German] Durch den geplanten Ausbau der Stromerzeugung aus fluktuierenden, erneuerbaren Energien in Deutschland ruecken Speichertechnologien fuer elektrische Energie immer staerker in den Fokus des industriellen und politischen Interesses. Eine vielversprechende Moeglichkeit fuer eine Langzeitspeicherung bei hohen Anteilen von Wind- und Photovoltaikanlagen sind Speichertechnologien wie die Wasserstoffspeicherung mit Hilfe von Wasserelektrolyseuren. Diese koennen erneuerbaren Strom in grossen Mengen und bei entsprechender Steuerung selektiv in Zeiten mit hohem Dargebot z.B. an Windstrom chemisch speichern. Der gespeicherte Wasserstoff kann dann entweder zu einem spaeteren Zeitpunkt wieder rueckverstromt oder direkt stofflich verwertet werden, z.B. als Kraftstoff fuer den Verkehrssektor, als chemischer Rohstoff oder fuer den Hausenergiebereich durch Einspeisung in das Erdgastransportnetz. Thema der vorliegenden Studie sind Analysen und Planungen fuer die Erprobung des Gesamtsystems ''Wasserstoff-Elektrolyse-Speicherung'' in energiewirtschaftlich relevanten Dimensionen. Dazu werden mit einem neuen Ansatz techno-oekonomische Entwicklungspfade fuer Wasserstoff-Systeme unterschiedlicher Groesse und Technologien charakterisiert und technologische Risiken bei der Realisierung dieser Systeme identifiziert und bewertet. Diese Arbeiten werden ergaenzt durch Arbeiten auf dem Gebiet der Salzkavernenspeicherung in Form

  13. [Crepidula beklemishevi gen. et sp. n. and Dimeiospora palustris gen. et sp. n. (Microspora: Amblyosporidae)--new microsporidian genera and species from blood-sucking mosquitoes (Diptera: Culicidae) from the south of the western Siberia].

    Science.gov (United States)

    Simakova, A V; Pankova, T F; Issi, I V

    2003-01-01

    Microsporidia parasitizing the adipose body of mosquito larvae of Anopheles beklemishevi and Aedes punctor has been studied. Two new genera of microsporidia are described based on lightmicroscopic and ultrastructural characteristics of spores and sporogony stages. The spore wall of Crepidula beklemishevi gen. n. et sp. n. is formed by two-membrane exospore, thick exospore, bilayer endospore and thin plasmolemma. Spores with single nucleus, polar filament anisofilar, with 6-7 coils (2+ 4-5), polaroplast consisting of three parts: macrochelicoidal, microhelicoidal and lamellar. Fixed spores 4.2 +/- 0.22 x 2 +/- 0.01 microns. The sporogony of Dimeiospora palustris gen. et. n. results in spore formation of two different types. Spores of the first type are oviform, with thick wall, single-nuclear, 6.1 x 4.9 microns. Spore wall with three layers, about 370 nm. Exospore electron-dense, subexospore moderately electrondense. Exospore and subexospore irregularly pleated on the almost spore surface and slightly thinner on anterior end only. Endospore electron-translucent. Polar filament anisofilar, with 9 coils (3 + 6). Polaroplas consists of three parts: lamellar, fine bubbled, and coarse bubbled. Spores of the second type broad-ovate, with apical pole narrower, distal pole concave, 4.6 x 3.7 microns. Spore wall with three layer, 355 nm. Exospore on the apical end irregularly pleated, consists of thin electrondense exospore, subexospore of variable electron density, endospore electron-translucent. Polar filament anisofilar, with 13 coils (3 + 10). Polaroplast has two parts: lamellar and vesicular.

  14. A 400-year phytolith-based reconstruction of wild rice (Zizania palustris) abundance from Mud Lake core sediments, Fond du Lac Band of Lake Superior Chippewa Reservation, Minnesota, USA.

    Science.gov (United States)

    Munoz, R.; Caylor, E.; Yost, C. L.; Drake, C.; Ladwig, J. L.; Myrbo, A.; Howes, T.

    2014-12-01

    Wild rice (Zizania palustris L.) is an aquatic grass with spiritual and subsistence significance to Native people of the Great Lakes region of North America. Mud Lake (Mashkiigwaagamaag), located on the Fond du Lac Band of Lake Superior Chippewa Reservation in Carlton County, Minnesota, USA, once supported an extensive population of wild rice (manoomin). However, early 20th century attempts to ditch and drain surrounding wetlands for landuse intensification severely altered the natural hydrological system that supports wild rice. Fond du Lac Resource Management (FDLRM) technicians are currently working to increase the wild rice population in Mud Lake. As part of these efforts, this phytolith study was undertaken to better understand how wild rice abundance has fluctuated over the past 400 years, with particular emphasis on the 19th and 20th centuries. Phytoliths are microscopic opal silica plant remains that are incorporated into soils and lake sediments after the plant-parts that contain them decay. Wild rice produces phytolith morphotypes that are unequivocally diagnostic. Mud Lake core MNMN-MUD11-1C-1P-1 (46°43'38.39"N, 92°42'2.45"W) was piston cored by LacCore (National Lacustrine Core Facility) and FDLRM technicians on 24 May 2011. Initial core descriptions, multi-sensor core logging, phytolith sampling and phytolith extractions were completed during the summer of 2014 at LacCore. Wild rice phytolith identification and quantification was conducted on twelve samples using brightfield microscopy at 400x magnification. Wild rice phytolith concentration values ranged from 68 to 2,300 phytoliths/cm3. Wild rice accumulation rates ranged from 9 to 383 phytoliths/ cm2/yr, peaking in 1952 AD. Wild rice abundance in Mud Lake appears to be influenced by a complex set of variables that include anthropogenic disturbance, climatic events and aquatic plant community succession.

  15. Climate protection by reducing the emissions of greenhouse gases in households and the tertiary sector through climate-conscious behaviour. Vol. 2; Klimaschutz durch Minderung von Treibhausgasemissionen im Bereich Haushalte und Kleinverbrauch durch klimagerechtes Verhalten. Bd. 2. Gewerbe, Handel und Dienstleistung

    Energy Technology Data Exchange (ETDEWEB)

    Boede, U.; Gruber, E. [Fraunhofer-Institut fuer Systemtechnik und Innovationsforschung (ISI), Karlsruhe (Germany); Deutscher, P.; Elsberger, M.; Rouvel, L. [Technische Univ., Muenchen (Germany). Lehrstuhl fuer Energiewirtschaft und Innovationsforschung; Brakhage, A.; Jaekel, M. [Abacus, Trier (Germany); Renner, G. [Innovative Energieberatung, Koeln (Germany); Brohmann, B.; Cames, M.; Herold, A.

    2000-08-01

    The aim of the project was to identify areas in households and the tertiary sector in which changes in behaviour could result in energy conservation and thus a reduction of greenhouse gas emissions, and to quantify the potentials for 1995, 2005 and 2020. A second focus was on the analysis and evaluation of programmes and instruments to realise the potentials. With literature evaluation, expert interviews, and a household servey potentials and further technical development have been identified. In sum, behavioural measures can contribute to the CO2 reduction by 64 million tons in 1995 in households and 27 in the commercial sector in which the potential decreases to 18 million tons in 2020 due to the autonomous technical development. Adequate promotion programmes can help to realise 20-30% of the potential by 2020. (orig.) [German] Ziel des Vorhabens war, im Sektor private Haushalte und Kleinverbrauch Bereiche zu identifizieren, in denen Verhaltensaenderungen zur Energieeinsparung fuehren koennen, und diese Potenziale fuer 1995, 2005 und 2020 zu quantifizieren. Darauf aufbauend waren Programme und Instrumente zur Umsetzung aufzuzeigen und zu bewerten. Gestuetzt auf Literaturrecherchen und Expertengespraeche wurden Einzelpotenziale, Rahmenbedingungen, Entwicklungstrends in der Technik und im Ausstattungsgrad ermittelt. Insgesamt koennten Verhaltensmassnahmen im Haushaltssektor die CO2-Emissionen im Basisjahr 1995 um 64 Mio, im Kleinverbrauch um 27 Mio t vermindern. Bis 2020 bleibt dieses Potenzial im Haushaltssektor in etwa gleich. Im Kleinverbrauch sinkt es infolge der autonomen Technikentwicklung auf 18 Mio t ab. Durch geeignete Programme koennen bis 2020 etwa 20-30% des Potenzials erreicht werden. (orig.)

  16. Protecting people and the environment - nuclear applications outside the energy sector; Schutz von Mensch und Umwelt durch nukleare Anwendungen ausserhalb des Energiesektors

    Energy Technology Data Exchange (ETDEWEB)

    Regge, P.P. de [PCI Lab., Internationale Atomenergie Organisation (IAEO), Wien (Austria)

    2002-07-01

    Sicherheitsnormen unterstuetzt. Folgende Schwerpunkte sind aufgrund ihrer besonderen Bedeutung fuer Mensch und Umwelt von besonderem Interesse: - Nuklearmedizin und Gesundheitsvorsorge - Veterinaermedizin und Viehzucht - Bodenkultivierung und Fuengung - Umweltschutz und Wasserversorgung - Pflanzenanbau - Schaedlingsbekaempfung - Landminenraeumung und Schutz von Kulturguetern. Die IAEO kann mit ihren Arbeiten und Erfahrungen von mehreren Jahrzehnten auf umfangreiche Erfolge verweisen. In vielen Laendern konnten durch die Entwicklung und Anwendung nuklearer Technologien z.B. wertvolle Beitraege zur Verbesserung der Ernaehrungssituation, des Gesundheitsschutzes und des Schutzes der Umwelt geleistet werden. Eine Fortfuehrung dieser Aktivitaeten der IAEO liegt im allgemeinen Interesse einer zukunftsweisenden internationalen Entwicklung und Zusammenarbeit. (orig.)

  17. Verminderung des Personalbedarfs durch den Einsatz einer computerbasierten Histologieprüfung [Reduction of the need for personel with a computer-based histology examination

    Directory of Open Access Journals (Sweden)

    Woermann, Ulrich

    2006-08-01

    Histologieprüfung insgesamt benötigten Personentage von 21 auf 6 zu reduzieren. Der materielle Aufwand ist mit 2 Prüfungs-PCs und einem Server-PC bescheiden. Sicherheitsprobleme bestehen bei einem in sich geschlossenen lokalen Netzwerk nicht. Durch kontinuierliche Speicherung der Prüfungsdaten auf dem Server wird ein Datenverlust vorgebeugt. Psychometrisch war die computerbasierte Histologieprüfung gleichwertig zur früher benutzten mündlich-strukturierten Prüfung. Der Einsatz von Computern kann den hohen Ressourcenbedarf von OSCE bzw. OSPE vermindern. Er kann somit mithelfen, dass ein wertvolles Prüfungsverfahren nicht an der beschränkten Verfügbarkeit von Personl scheitert.

  18. Instruments. Climate protection of reduction of no-load losses in electric appliances and equipment; Klimaschutz durch Minderung von Leerlaufverlusten bei Elektrogeraeten. Instrumente

    Energy Technology Data Exchange (ETDEWEB)

    Rath, U.; Hellmann, R. [Ingenieurbuero fuer Energieberatung, Haustechnik und Oekologische Konzepte GbR (eboek), Tuebingen (Germany); Moehring-Hueser, W.; Wortmann, K.; Bregas, J. [Energiestiftung Schleswig-Holstein, Kiel (Germany); Mordziol, C. [Umweltbundesamt, Berlin (Germany)

    1999-12-01

    Two studies on the subject of 'Climate Protection through Reduction of No-load Losses in Electric Applicances and Equipment' have been performed on behalf of the German Federal Ministry for the Environment, Nature Conservation and Nuclear Safety and the German Federal Environmental Agency. The first study, carried out by the Tuebingen-based engineering bureau eboek, has been published in the Federal Environmental Agency's TEXTE series (Texte 45/97, 2{sup nd} edition, 1998). It was the starting point for a multitude of activities among them two information campaigns carried out in 1998 and 1999 that were funded by the Federal Ministry for the Environment and the German Section of the Friends of the Earth (BUND). The study also served as a basis for decisions taken by the German Bundestag and the Bundesrat (Council of Constituent States). The second study on the subject was carried out by the engineering bureau eboek and Energiestiftung Schleswig-Holstein. Building on the results obtained in the first study, it presents estimates of relevant data for the European Union and examines various approaches for possible measures to reduce no-load losses as to their suitability. The studies impressively show that effective climate protection can be achieved in all relevant sectors, and in many cases even save costs. The results are detailed below. (orig.) [German] Im Auftrag des Bundesministeriums fuer Umwelt, Naturschutz und Reaktorsicherheit sowie des Umweltbundesamtes wurden zwei Studien zum Thema: 'Klimaschutz durch Minderung von Leerlaufverlusten bei Elektrogeraeten' durchgefuehrt: Die erste, vom Ingenieurbuero fuer Energieberatung, Haustechnik und oekologische Konzepte (eboek), Tuebingen, earbeitete Studie wurde in der Reihe TEXTE des UBA veroeffentlicht (Texte 45/97, 2. Auflage 1998). Sie bildete den Ausgangspunkt fuer eine Vielzahl von Aktivitaeten, unter anderem zwei vom Bundesumweltministerium finanzierte und vom Bund fuer Umwelt und

  19. Herausforderungen durch die deutsche Wiedervereinigung

    Science.gov (United States)

    Stäglin, Reiner

    Die Wiedervereinigung stellte auch die Statistik vor große Aufgaben. Die als Organ der staatlichen Planung staatsnah orientierte Statistik der DDR musste auf das zur Neutralität und wissenschaftlichen Unabhängigkeit verpflichtete System der Bundesrepublik umgestellt werden. Ebenso verlangten die Universitäten eine Neuorientierung. Die Deutsche Statistische Gesellschaft hat sich vor allem dreier Aufgaben mit großem Engagement, aber auch mit Bedachtsamkeit angenommen: Aufnahme und Integration der Statistiker aus den neuen Bundesländern in die Gesellschaft, Begleitung der Neuausrichtung des Faches Statistik an deren Hochschulen und Sicherung sowie Nutzung von Datenbeständen der ehemaligen DDR.

  20. Low-cost development of the Algyoefield in Hungary by means of horizontal re-entry boreholes; Die kostenguenstige Entwicklung des Algyoe-West-Feldes in Ungarn durch horizontale Re-Entry-Bohrungen

    Energy Technology Data Exchange (ETDEWEB)

    Kinzel, H. [Weatherford Oil Tool GmbH, Langenhagen (Germany); Osz, A. [Magyar Olaj (MOL), Budapest (Hungary); Kerk, T. [Becfield Drilling Services, Edemissen-Berkhoepen (Germany)

    1998-12-31

    Planning and implementation of a typical re-entry borehole in southern Hungary are described. The cost is about half that of a new borehole, and the available infrastructure can be used. The production index of a typical Algyoehorizontal borehole is higher by a factor of 12 than for a vertical borehole in the same field. (orig.) [Deutsch] Anhand einer Beispielbohrung (Algyoe488) wird die Planung und Durchfuehrung einer typischen Re-Entry Bohrung in Suedungarn beschrieben. Durch die weitgehende Verwendung von standardisierten Komponenten und Verfahren sowie durch die enge Zusammenarbeit zweier deutscher Service Unternehmen mit dem Auftraggeber wurde bei insgesamt 35 Horizontalbohrungen in Ungarn der Effekt der Lernkurve zur optimierten Erstellung der Bohrung und damit zur Kostensenkung effektiv eingesetzt. Die so aufgearbeiteten Bohrungen werden im Vergleich zu einer neuen Bohrung fuer etwa die Haelfte der Kosten erstellt. Die vorhandene Infrastruktur des Feldes kann weiter verwendet werden. Der Produktionsindex einer typischen AlgyoeHorizontalbohrung liegt um den Faktor 12 hoeher als eine Vertikalbohrung im gleichen Feld. (orig.)

  1. Selective network protection and economically efficient network operation in underground areas with high explosion hazard by automation of protection systems. Final report; Selektiver Netzschutz und wirtschaftlicher Netzbetrieb in schlagwettergefaehrdeten Bereichen durch Automatisierung der Schutztechnik. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Eickhoff, F. [Deutsche Montan Technologie GmbH, Bochum (Germany). Gas and Fire Division

    2002-07-01

    Electric power supply systems for coal mines in Germany from 2003 must adapt to developments in mining and to changing standards. Future systems should be more efficient, more compact, and with less network losses. Automatic network protection of underground networks of 5 kV and more is problematic due to economic considerations. Advantages are found in higher safety, protective earthing and faster information on disturbances. Economic considerations and formal boundary conditions concerning the acceptance of explosion protection systems and electrotechnical safety requirements permit only automation of existing switches with the aid of available protection systems. [German] Zur Realisierung einer elektrischen Energieversorgung im Steinkohlenbergbau in Deutschland fuer die Jahre bis 2003 und spaeter wird eine Anpassung an die bergtechnischen Entwicklungen notwendig. Zudem muss eine Anpassung an geaenderte Normenvorgaben erfolgen. In der Energieversorgung sind Entwicklungen im Gange, die Versorgung anders zu strukturieren, um den Abbaubetrieb leistungsfaehiger, kompakter und verlustaermer mit elektrischer Energie versorgen zu koennen. Die weitergehende Automatisierung des Netzschutzes ab 5 kV im Bereich unter Tage ist aufgrund der wirtschaftlichen Situation nur eingeschraenkt durchfuehrbar. Die moeglichen Sicherheitsvorteile liegen nach Auswertung bisheriger Forschungsvorhaben im Verhaeltnis von Ergebnis und Aufwand insbesondere: - im Erdschlussschutz der Hochspannungsnetze und - in der Stoerungsklaerung durch detaillierte Informationen des Netzschutzes. Die wirtschaftlichen Vorgaben und formalen Randbedingungen bei der Zulassung bezueglich Explosionsschutz und elektrotechnischer Sicherheitsanforderungen erlauben daher nur eine Automatisierung vorhandener Schalter durch Verwendung entsprechender vorhandener Schutzgeraete. (orig.)

  2. Energy conservation in refrigerating machines by combining of thermo-static expansion valve and interior heat transfer material; Energieeinsparung in Kaelteanlagen durch Kombination von thermostatischem Expansionsventil und innerem Waermeuebertrager

    Energy Technology Data Exchange (ETDEWEB)

    Tambovtsev, Alexander

    2007-12-15

    bekannten Komponenten TEV und IWUeals inkompatibel miteinander, weil die einfache Aneinanderreihung zu einem instabilen Regelverhalten fuehrt. Nach detaillierter Analyse der Ursachen dieser Instabilitaet wird die Hypothese aufgestellt, dass durch eine absichtliche ''Verschlechterung'' des IWUeein stabiler Arbeitsbereich gefunden werden kann. Bei dieser ''Verschlechterung'' kann es sich z. B. um den Bypass eines Teils des Hochdruckstromes oder um eine Gleichstromanordnung der Stroeme im Waermeuebertrager handeln. Diese Hypothese wurde primaer durch Experimente, aber zusaetzlich auch durch Simulationsrechnungen bestaetigt. Dafuer wurde ein Versuchsstand aufgebaut, mit welchem verschiedene Konfigurationen und Regelstrategien getestet wurden. Es wurde eine deutliche Verringerung des Energieverbrauchs gegenueber herkoemmlichen Anordnungen erreicht. (orig.)

  3. Remediation of heavy-metal contaminated sediments by means of bioleaching. Study: project results - economic efficiency - market assessment. Final report; Reinigung schwermetallbelasteter Sedimente durch Bioleaching. Studie: Projektergebnisse - Wirtschaftlichkeit - Marktbewertung. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Loeser, C.; Zehnsdorf, A.; Hoffmann, P.; Seidel, H.; Schmerold, R.

    2002-07-01

    Sedimente mit Sauerstoff in Kontakt, gehen die Schwermetalle durch mikrobielle Oxidations- und Versauerungsprozesse teilweise in Loesung. Dieses natuerliche Bioleaching, welches unkontrolliert ein Gefahrenpotential fuer die Umwelt darstellt, soll durch Aktivierung der autochthonen schwefeloxidierenden Bakterien (z.B. Thiobacilli) beschleunigt und zur Reinigung der Sedimente genutzt werden. Ziel ist es, ein naturnahes Verfahren zur Sedimentdekontamination unter Nutzung des natuerlichen biologischen Solubilisierungspotentials bereitzustellen. Die gereinigten Sedimente sollen in den Stoffkreislauf als Bodensubstrat zurueckgefuehrt werden. Die Verfahrensentwicklung erfolgte am Beispiel von hochbelasteten Sedimenten der Weissen Elster aus dem Suedraum Leipzig. Aus oekonomischen Gruenden soll das Bioleaching der Schwermetalle durch Festbettlaugung nach dem Perkolationsprinzip erfolgen. Das Verfahren erfordert mehrere Prozessstufen. Der Schwerpunkt der Untersuchungen im Labor- und Technikumsmassstab lag auf der Erarbeitung und technischen Umsetzung von Massnahmen zur Beschleunigung der Metallsolubilisierung. (orig.)

  4. Measurements of size distributions of radon progeny for improved quantification of the lung cancer risk emanating from exposure to radon decay products; Messungen der Groessenverteilungen von Radon-Folgeprodukten zur Verbesserung der Quantifizierung des durch Radonexposition verursachten Lungenkrebsrisikos

    Energy Technology Data Exchange (ETDEWEB)

    Haninger, T.

    1997-12-31

    A major issue in radiation protection is to protect the population from the harmful effects of exposure to radon and radon progeny. Quantification of the lung cancer risk emanating from exposure to radon decay products in residential and working environments poses problems, as epidemiologic studies yield information deviating from the results obtained by the indirect method of assessment based on dosimetric respiratory tract models. One important task of the publication here was to characterize the various exposure conditions and to quantify uncertainties that may result from application of the ``dose conversion convention``. A special aerosol spectrometer was therefore designed and built in order to measure the size distributions of the short-lived radon decay products in the range between 0.5 nm and 10 000 nm. The aerosol spectrometer consists of a three-step diffusion battery with wire nets, an 11-step BERNER impactor, and a detector system with twelve large-surface proportional detectors. From the measured size distributions, dose conversion coefficients, E/P{sup eq}, were calculated using the PC software RADEP; the RADEP program was developed by BIRCHALL and JAMES and is based on the respiratory tract model of the ICRP. The E/P{sup eq} coefficients indicate the effective dose E per unit exposure P{sup eq} to radon decay products. (orig./CB) [Deutsch] Eines der groessten Probleme des Strahlenschutzes ist der Schutz der Bevoelkerung vor einer Strahlenexposition durch Radon und seine Folgeprodukte. Die Quantifizierung des Lungenkrebsrisikos, das durch Radonexpositionen in Wohnungen und an Arbeitsplaetzen verursacht wird, ist ein grosses Problem, weil epidemiologische Studien ein anderes Ergebnis liefern, als die indirekte Methode der Abschaetzung mit dosimetrischen Atemtrakt-Modellen. Eine wichtige Aufgabe der vorliegenden Arbeit war es, unterschiedliche Expositionsbedingungen zu charakterisieren und die Unsicherheiten zu quantifizieren, die sich aus der

  5. Enhanced biological phosphorus removal under alternating anaerobic and anoxic conditions. Pt. 2. The sorption-denitrification-P-removal-system; Vermehrte biologische Phosphorelimination durch alternierende anaerob/anoxische Milieubedingungen. T. 2. S-DN-P-Biofilmverfahren

    Energy Technology Data Exchange (ETDEWEB)

    Sieker, C.A. [Berliner Wasserbetriebe, Berlin (Germany); Hegemann, W. [Technische Univ. Berlin (Germany). Fachgebiet Siedlungswasserwirtschaft

    2000-07-01

    The Sorption-Denitrification-P-Removal-system (S-DN-P-system) is a process which combines biological excess P-removal and denitrification in a biofilm system. The accumulation of polyP organisms is achieved by sequencing anaerobic and anoxic conditions. The biofilm is in alternating contact with primary treated wastewater (anaerobic phase) and nitrified wastewater (anoxic phase). In the anaerobic phase, P-Release takes place and readily biodegradable organic substrate is fermented to volatile fatty acids and absorbed by the polyP organisms. In addition to this, other organic substrate is physically/chemically adsorbed in the biofilm. In the anoxic phase the biofilm dentirifies the ab/adsorbed organic substrate and, at the same time, P-uptake and polyP formation occurs. The research on the S-DN-P-system shows that P-removal in the anaerobic/anoxic system works well and ab- and adsorption mechanisms nearly remove all of the readily biodegradable substrate in the anaerobic phase. Consequently, this substrate can be used for denitrification in the anoxic phase. The S-DN-P-Process is a very promising concept. However, up to now the technical scale-up is unclear. This is subject to further investigation at the Technical University of Berlin. (orig.) [German] Das S-DN-P-Verfahren ist ein Biofilmverfahren, welches die vermehrte biologische Phosphorelemination und die Denitrifikation kombiniert. Die Anreicherung von denitrifizierenden polyP-Organismen wird durch alternierende anaerob-anoxische Milieubedingungen erreicht. Der Biofilm wird abwechselnd mit vorgeklaertem Abwasser beschickt (Sorptionsphase) und nitrifiziertem Abwasser (Denitrifikationsphase). In der anaeroben Sorptionsphase erfolgt eine P-Rueckloesung sowie die Aufnahme und zellinterne Speicherung von organischen Saeuren durch polyP-Organismen. Darueber hinaus werden andere organische Substanzen im Biofilm chemisch/physikalisch adsorbiert. In der Denitrifikationsphase werden die zuvor gespeicherten und

  6. Measurements of size distributions of radon progeny for improved quantification of the lung cancer risk emanating from exposure to radon decay products; Messungen der Groessenverteilungen von Radon-Folgeprodukten zur Verbesserung der Quantifizierung des durch Radonexposition verursachten Lungenkrebsrisikos

    Energy Technology Data Exchange (ETDEWEB)

    Haninger, T.

    1997-12-31

    A major issue in radiation protection is to protect the population from the harmful effects of exposure to radon and radon progeny. Quantification of the lung cancer risk emanating from exposure to radon decay products in residential and working environments poses problems, as epidemiologic studies yield information deviating from the results obtained by the indirect method of assessment based on dosimetric respiratory tract models. One important task of the publication here was to characterize the various exposure conditions and to quantify uncertainties that may result from application of the ``dose conversion convention``. A special aerosol spectrometer was therefore designed and built in order to measure the size distributions of the short-lived radon decay products in the range between 0.5 nm and 10 000 nm. The aerosol spectrometer consists of a three-step diffusion battery with wire nets, an 11-step BERNER impactor, and a detector system with twelve large-surface proportional detectors. From the measured size distributions, dose conversion coefficients, E/P{sup eq}, were calculated using the PC software RADEP; the RADEP program was developed by BIRCHALL and JAMES and is based on the respiratory tract model of the ICRP. The E/P{sup eq} coefficients indicate the effective dose E per unit exposure P{sup eq} to radon decay products. (orig./CB) [Deutsch] Eines der groessten Probleme des Strahlenschutzes ist der Schutz der Bevoelkerung vor einer Strahlenexposition durch Radon und seine Folgeprodukte. Die Quantifizierung des Lungenkrebsrisikos, das durch Radonexpositionen in Wohnungen und an Arbeitsplaetzen verursacht wird, ist ein grosses Problem, weil epidemiologische Studien ein anderes Ergebnis liefern, als die indirekte Methode der Abschaetzung mit dosimetrischen Atemtrakt-Modellen. Eine wichtige Aufgabe der vorliegenden Arbeit war es, unterschiedliche Expositionsbedingungen zu charakterisieren und die Unsicherheiten zu quantifizieren, die sich aus der

  7. Einheitliche Dosis-Messgrössen durch die Umsetzung der Richtlinie 96/29/EURATOM. Auswirkungen auf die Dartstellung und die Weitergabe der Einheit Sievert für die Messgrössen, auf die Messtechnik und auf Bauartprüfungen

    CERN Document Server

    Ambrosi, P; Ankerhold, U; Behrens, R; Böhm, J; Helmstädter, K; Schuhmacher, H; Wissmann, F; Zimbal, A

    2003-01-01

    Einheitliche Dosis-Messgrössen durch die Umsetzung der Richtlinie 96/29/EURATOM. Auswirkungen auf die Dartstellung und die Weitergabe der Einheit Sievert für die Messgrössen, auf die Messtechnik und auf Bauartprüfungen

  8. Superconductors for the medium-voltage grid. A superconducting power cable running through the inner city of Essen passes a two-year field test; Supraleiter fuer das Mittelspannungsnetz. Ein supraleitendes Stromkabel quer durch die Essener Innenstadt besteht zweijaehrigen Feldtest

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, Franz

    2017-04-01

    Scientists are testing the longest high-temperature superconducting cable in the world under real conditions in Essen. One kilometre long, it connects two substations in the inner city. It replaces a conventional 110 kV line and renders one substation in the inner city obsolete. After two years of testing, it has passed the field test. It could be a blueprint for the future power supply system in urban areas. [German] Wissenschaftler testen in Essen das laengste Hochtemperatur-Supraleiterkabel der Welt unter realen Bedingungen. Mit einer Laenge von einem Kilometer verbindet es zwei Umspannstationen quer durch die Innenstadt. Es ersetzt eine konventionelle 110-kV-Leitung und macht eine Umspannanlage im Stadtzentrum ueberfluessig. In einer zweijaehrigen Erprobung hat es den Praxistest bestanden. Es koennte eine Blaupause fuer die kuenftige Stromversorgung in Ballungsraeumen sein.

  9. Mathematical modelling of transport of gaseous and liquid substances induced by pyrite oxidation in spoil banks of lignite mines; Mathematische Modellierung der durch Pyritoxidation induzierten Stofftransporte in Braunkohleabraumkippen in gasfoermiger und fluessiger Phase

    Energy Technology Data Exchange (ETDEWEB)

    Ludwig, R.

    2001-07-01

    Pyrite (FeS{sub 2}) oxidation due to open-pit lignite mining activities may result in acid mine drainage and dissolved metals in the soil and ground water of the overburden spoil piles. The pyrite oxidation takes place firstly during the mining process and at the temporary surfaces of the mine, secondly in deep zones of the overburden spoil pile as a result of gas transport through the unsaturated zone. The second process continues after mine closure possibly over several decades and therefore contaminates the environment for a long time. Whereas some studies deal with a minimisation by geochemical means, this research focuses on a minimisation by impacting the physical boundary conditions. Special attention is given to the transport of oxygen from the soil surface to the pyrite oxidation zone. Soil column experiments with columns of 2.90 m height yield experimental data concerning the pyrite oxidation and the related transport processes in the unsaturated zone. Experimental data are compared to simulation results obtained with the model MUSIC; it calculates coupled reaction and transport processes in a one-dimensional soil profile using the finite volume method. Pyrite oxidation is described by means of a first order kinetics, the gas transport takes place by diffusion as well as convection. The transport of solutes in the soil water is described by the convection-dispersion equation (Cl{sup -}, Fe{sup 2+} and SO{sub 4}{sup 2-}). The simulations are able to reproduce the measured time series of oxygen concentrations in the soil profile. (orig.) [German] Die durch Braunkohlegewinnung im Tagebau hervorgerufene Pyritoxidation (Pyrit: FeS{sub 2}) kann zu Versauerung, Schwermetallfreisetzung und Aufmineralisierung des Boden- und Grundwassers in den Abraumkippen fuehren. Die Pyritoxidation findet zum einen waehrend des Abbaubetriebs und an den temporaeren Oberflaechen des Tagebaus statt, zum anderen in tieferen Bereichen der Abraumkippe durch Gastransport durch die

  10. Rezension von: Ulf Gebken, Söhnke Vosgerau (Hg.: Fußball ohne Abseits. Ergebnisse und Perspektiven des Projekts ‚Soziale Integration von Mädchen durch Fußball‘. Wiesbaden: Springer VS 2014.

    Directory of Open Access Journals (Sweden)

    Robert Claus

    2014-12-01

    Full Text Available Seit 2006 wurden im Rahmen des vom Deutschen Fußball-Bund durchgeführten Modellprojektes „Soziale Integration von Mädchen durch Fußball“ an über 200 Standorten Schul-AGs in sogenannten Brennpunktbezirken aufgebaut. Im vorliegenden Sammelband beleuchten die Autor/-innen ausführlich die Funktion von Sport für die pädagogische Kompetenzvermittlung. Zudem erbringen sie einen umfassenden Projektbericht und leisten ein Plädoyer für die Modernisierung des Verhältnisses zwischen Schule und Vereinen, Lehrplan und Ehrenamt. Somit stellt das Buch, wie auch das Projekt, einen enorm wichtigen Beitrag zur Geschlechtergerechtigkeit im Sport dar. Gleichzeitig jedoch liest sich eine Reihe an Texten etwas zu sperrig für den Bericht einer Praxisforschung, und Begriffe im Feld der ‚Integration’ bleiben schwammig.

  11. Molecular Regulation of Photosynthetic Carbon Dioxide Fixation in Nonsulfur Purple Bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Tabita, Fred Robert [The Ohio State Univ., Columbus, OH (United States)

    2015-12-01

    The overall objective of this project is to determine the mechanism by which a transcriptional activator protein affects CO2 fixation (cbb) gene expression in nonsulfur purple photosynthetic bacteria, with special emphasis to Rhodobacter sphaeroides and with comparison to Rhodopseudomonas palustris. These studies culminated in several publications which indicated that additional regulators interact with the master regulator CbbR in both R. sphaeroides and R. palustris. In addition, the interactive control of the carbon and nitrogen assimilatory pathways was studied and unique regulatory signals were discovered.

  12. Obstructed surgical porto-systemic shunts in the early postoperative period: interventional therapy by angioplasty (PTA) and stent placement; Obstruktion chirurgischer portosystemischer Shunts in der fruehen postoperativen Phase: interventionelle Behandlung durch Angioplastie und Stentimplantation

    Energy Technology Data Exchange (ETDEWEB)

    Strunk, H.; Textor, J.; Koenig, R.; Wilhelm, K.; Schild, H. [Bonn Univ. (Germany). Radiologische Klinik; Wolff, M. [Bonn Univ. (Germany). Chirurgische Klinik und Poliklinik

    2001-05-01

    Recurrent variceal bleeding in patients treated with surgical porto-systemic shunting is most often due to shunt stenoses or occlusion. Radiological interventional procedures are a possible method of therapy and our experience herein is described in this report. Patients and Methods: from 1997 to 1999 54 patients with recurrent variceal bleeding were treated with a surgical porto-systemic shunt procedure. Of these early shunt occlusion occurred in 5 patients, which was treated with percutaneous transcatheter techniques. Results: in only one patient was PTA alone sufficient to reestablish shunt patency, in four patient stent placement was necessary in addition. In the follow-up period 1 patient died 26 month after intervention with (autopsy-proven) patent shunt, in one patient shunt reocclusion occurred after 11 months and in 3 patients the shunt is still patent. Conclusions: PTA, if necessary in combination with stent placement, is an attractive alternative method of therapy in case of an early surgical porto-systemic shunt occlusion. (orig.) [German] Rezidivblutungen nach chirurgischer portosystemischer Shuntanlage sind meistens durch eine Shuntstenose oder einen Shuntverschluss bedingt. Hier stellen interventionelle Eingriffe einen moeglichen therapeutischen Ansatz dar, ueber den im Folgenden berichtet werden soll. Patienten und Methode: zwischen 1997 und 1999 wurde bei insgesamt 54 Patienten wegen rezidivierender Blutungen ein operativer portosystemischer Shunt angelegt. Von diesen zeigten fuenf Patienten in der unmittelbaren postoperativen Periode einen Shuntverschluss, der radiologisch interventionell therapiert wurde. Ergebnisse: bei einem der fuenf Patienten genuegte zur Wiederherstellung des Flusses eine alleinige Ballondilatation (PTA), bei vier Patienten musste zusaetzlich eine Stentimplantation durchgefuehrt werden. In der weiteren Nachsorge ist ein Pat. nach 26 Wochen mit autoptisch offenem Shunt verstorben, bei 3 Pat. ist der Shunt offen, bei einem

  13. Evaluation of measures of the German Ministry of Economics (BMWi) in support of enhanced use of renewable energy technologies (1994 - 1998). Final report; Evaluierung der Foerderung von Massnahmen zur Nutzung erneuerbarer Energien durch das Bundesministerium fuer Wirtschaft (1994 - 1998). Endbericht

    Energy Technology Data Exchange (ETDEWEB)

    Reichert, J.; Gruber, E.; Mannsbart, W.

    1999-01-01

    The document presents an analysis of a governmental support programme for enhanced use of renewable energy technologies, installed for the period from 1994 to 1998. The '100 million Deutschmark programme' financed by the German Federal Ministry of Economics (BMWi) was intended to boost applications of renewable energy technologies in the residential and commercial sector. The objectives of the programme were to improve market penetration of renewable energy technologies, eg. by inducing a decline in costs, so as to bring those generation technologies closer to the break-even point of economic efficiency, or make them achieve economic efficiency on a broader basis through more widespread applications. The evaluation presented in the document was to show the programme's achievements with respect to the following aspects and criteria: Comparison with other governmental programmes, in particular those of Land governments. Indirect effects on employment, imports and exports, or in terms of incentives for technological enhancements. Assessment of direct effects on the marketing situation and the technologies. The conclusions drawn were to include recommendations for a successor programme. (orig./CB) [German] Vom Bundesministerium fuer Wirtschaft (BMWi) wurde die Nutzung erneuerbarer Energien durch Investitionszuschuesse fuer private Haushalte und Unternehmen von 1994 bis 1998 in einem '100-Millionen-DM-Programm' gefoerdert. Es war als Breitenprogramm ausgelegt, sollte die Nachfrage staerken und Marktimpulse in Form von Kostendegressionen geben, so dass die erneuerbaren Energien der Wirtschaftlichkeit naeher kommen oder sie erreichen. Die vorliegende Evaluation sollte den Erfolg des Programmes durch Aussagen zu den nachstehenden Themenschwerpunkten analysieren: - Vergleich des Foerderprogramms mit anderen Programmen, insbesondere denjenigen auf Laenderebene. - Die indirekten Wirkungen auf Arbeitsplaetze, Import und Export, Anstoss fuer technische

  14. Lignin removal from paper mill wastewaters in sequencing batch reactors (SBR) by adsorption to the sludge; Entfernung von Lignin aus Papierfabrikabwaessern in Sequencing-Batch-Reaktoren (SBR) durch Schlamm-Adsorption

    Energy Technology Data Exchange (ETDEWEB)

    Schiegl, C.; Kriebitzsch, K.; Helmreich, B.; Wilderer, P.A. [Technische Univ. Muenchen, Garching (Germany). Lehrstuhl und Pruefamt fuer Wasserguete- und Abfallwirtschaft

    1997-11-01

    Lignin is known to be poorly biodegradable. During the mechanical pulp lignin gets dissolved in the water because of high temperatures and pressures. By biological treatment of mechanical pulp wastewater the amount of lignin is decreased. Experiments about lignin adsorption were carried out in a lab scale batch-test with different sludge ages to clarify whether lignin is removed from the waste water by biodegradation or by adsorption to the activated sludge. By carrying out pyrolysis-gas chromatography/mass spectrometry (pyGC/MS) the amount of lignin was quantified for the different sludges. Up to 30 weight percent of lignin could be adsorbed to the activated sludge which proves the excellent adsorption properties of the activated sludge. The adsorbed quantity of lignin depends on the sludge age which results in a higher adsorptive capacity of the sludge at lower sludge ages than at higher ones. A total removal of the lignin from the wastewater could be reached if there was a satisfying high content of sludge for total lignin adsorption or a relatively low amount of lignin in the influent. (orig.) [Deutsch] Lignin gilt als biologisch schwerabbaubar. Beim Holzschliffprozess wird aufgrund der hohen Temperaturen und Druecke Lignin im Wasser geloest. Durch die biologische Reinigung von Holzschliffabwasser in Sequencing-Batch Reaktoren (SBR) kann die Menge an geloestem Lignin reduziert werden. Um zu klaeren, ob Lignin biologisch metabolisiert wird oder aber lediglich durch Adsorption an Schlamm aus dem Abwasser entfernt wird, werden Batch-Versuche zur Adsorption des Lignin im Labormassstab mit verschiedenen Schlammaltern durchgefuehrt. Mit Hilfe der Pyrolyse-Gaschromatographie/Massenspektrometrie (pyGC/MS) wird der Lingingehalt der einzelnen Schlaemme quantifiziert. Der Belebtschlamm erweist sich dabei als hervorragendes Adsorbermaterial, an dem sich Lignin mit bis zu 30 Gewichtsprozent adsorbieren laesst. Die Adsorptionskapazitaet der einzelnen Schlaemme sinkt mit

  15. Integrating large-scale functional genomics data to dissect metabolic networks for hydrogen production

    Energy Technology Data Exchange (ETDEWEB)

    Harwood, Caroline S

    2012-12-17

    The goal of this project is to identify gene networks that are critical for efficient biohydrogen production by leveraging variation in gene content and gene expression in independently isolated Rhodopseudomonas palustris strains. Coexpression methods were applied to large data sets that we have collected to define probabilistic causal gene networks. To our knowledge this a first systems level approach that takes advantage of strain-to strain variability to computationally define networks critical for a particular bacterial phenotypic trait.

  16. Radiation exposure to the patient caused by single-photon transmission measurement for 3D whole-body PET; Die Strahlenexposition des Patienten durch die Einzelphotonen-Transmissionsmessung bei der PET

    Energy Technology Data Exchange (ETDEWEB)

    Schaefer, A.; Donsch, P.; Kirsch, C.M. [Universitaet des Saarlandes, Homburg/Saar (Germany). Abt. fuer Nuklearmedizin; Seifert, H. [Universitaet des Saarlandes, Homburg/Saar (Germany). Abt. Strahlentherapie der Radiologischen Klinik

    2000-11-01

    Patienten durch die Transmissionsmessung mittels Einzelphotonenquellen bei der PET. Material und Methode: Zwei Cs-137-Punktquellen (E{gamma}=662 keV, A=614 MBq) werden zur Transmissionsmessung im Einzelphotonenmodus an einem 3D-Scanner (ECAT ART) eingesetzt. Bei der Simulation einer Ganzkoerper-Transmission (axiale Laenge: 75 cm, 6 ueberlappende Bettpositionen), werden Dosismessungen mit Thermolumineszenzdosimetern unter Verwendung eines Thorax- und eines Abdomenphantoms durchgefuehrt. Aus den Messwerten wurde in Anlehnung an den Report Nr. 60 der ICRP die effektive Dosis fuer die Transmissionsmessung abgeschaetzt. Ergebnisse: Bei einer Gesamtaufnahmedauer von 360 min (60 min pro Bettposition) ergaben sich folgende Energiedosen: Oberflaeche (Xyphoid) 189 {mu}Gy, Herz 196 {mu}Gy, Lunge 234 {mu}Gy, BWS 240 {mu}Gy, Niere 207 {mu}Gy, Leber 204 {mu}Gy, Gonaden 205 {mu}Gy, Schilddruese 249 {mu}Gy und Blase 185 {mu}Gy, aus denen sich ein Konversionsfaktor von 1,7*10{sup -4} mSv/(h*MBq) errechnete. Die Abschaetzung der effektiven Dosis fuer den Patienten aufgrund einer Transmissionsmessung (Akquisitionszeit von 3,2 min pro Bettposition) ergab einen Wert von 11 {mu}Sv. Die Abschaetzung des Verhaeltnisses der Konversionsfaktoren durch Transmissionsmessung im Einzelphotonen- und im Koinzidenzmodus (zwei Ge-68/Ga-68-Linienquellen mit jeweils 40 MBq) ergab einen Wert von 0,18. Der Vergleich zwischen den effektiven Dosen durch die Transmission im Einzelphotonen-Modus und die Emission (bei Injektion von 250 MBq FDG) ergab ein Verhaeltnis von 2,3*10{sup -3}. Schlussfolgerung: Die Strahlenexposition der Patienten durch die Transmissionsmessung in der 3D-PET ist vernachlaessigbar gering. Sie wird durch die Verwendung der Einzelphotonenmethode mit kollimierten Punktquellen relativ hoher Aktivitaet im Vergleich zur Koinzidenzmethode mit nicht-kollimierten Linienquellen relativ niedriger Aktivitaet weiter reduziert. (orig.)

  17. Reduction of the acoustic noise emission of wind turbines by modification of rotor bade tip shapes, trailing edges and pitch angles. Final report; Geraeuschminderung durch Modifikation der Blattspitze, der Blatthinterkante und des Anstellwinkels von Windkraftanlagen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Betke, K. [Oldenburg Univ. (Germany). Inst. fuer Technische und Angewandte Physik; Gabriel, J.; Klug, H. [Deutsches Windenergie-Institut gGmbH (DEWI), Wilhelmshaven (Germany); Schumacher, K.; Wittwer, G. [Abeking und Rasmussen Faserverbundtechnik GmbH, Lemwerder (Germany); Litzka [Foerdergesellschaft Windenergie e.V., Brunsbuettel (Germany); Seel [Seewind Windenergiesysteme GmbH, Walzbachtal (Germany); Petersen; Partmann [Tacke Windtechnik GmbH und Co. KG, Rheine (Germany); Kolbert [Ventis Energietechnik GmbH, Braunschweig (Germany)

    1997-12-31

    In cooperation with manufacturers of wind turbines and rotor blades acoustic measurements on modified tip shapes, trailing edges and pitch angles were performed in a wind tunnel and on real turbines. The results provide manufacturers, developers and operators of wind turbines with a tool to reduce the noise radiation from wind turbines. Significant noise reductions were obtained by tip shape modifications. The sound power level of a modified 600 kW wind turbine was less than 98 dB(A). Modifications of the trailing edge were less effective than expected from theory. Sharp trailing edges were almost as effective as serrated trailing edges but both require special procedures in production and handling. Pitch angle modifications led to significant noise reductions when the angle of attack was reduced. The power curve of the modified turbine was measured and resulted in an energy loss in the range of 1% while the noise was reduced by 2 dB(A). (orig.) [Deutsch] In Zusammenarbeit mit Herstellern von Windenergieanlagen und Rotorblaettern wurden akustisch wirksame Modifikationen der Blattspitze, der Blatthinterkante und des Blatteinstellwinkels im Windkanal vermessen. Die gewonnenen Erkenntnisse wurden bei unterschiedlichen realen Windenergieanlagen umgesetzt und die erzielte Geraeuschminderung durch direkte Vergleichsmessungen vor Ort bestimmt. Aus den Ergebnissen des Projektes lassen sich allgemeine Aussagen ueber Geraeuschminderungsmassnahmen an Windenergieanlagen ableiten. Es hat sich gezeigt, dass bei vielen Windenergieanlagen deutliche Reduzierungen der Geraeuschabstrahlung durch eine Veraenderung der Blattspitzenform erzielt werden koennen (erzwungener Umschlag) vermieden werden kann. Die Modifikation der Blattspitze fuehrte zu dem niedrigsten Schalleistungspegel in der 600 kW - Leistungsklasse von unter 98 dB(A). Bei den Modifikationen der Hinterkante konnte der erhoffte Erfolg bei saegezahnfoermigen Hinterkanten im Windkanal nicht nachgewiesen werden. Im

  18. Measurement of in-vivo dosage increase due to dental alloys during therapeutic irradiation of the mouth cavity; In-vivo-Bestimmung der Dosiserhoehung durch Dentallegierungen bei therapeutischer Bestrahlung der Mundhoehle

    Energy Technology Data Exchange (ETDEWEB)

    Thilmann, C. [Frankfurt Univ. (Germany). Abt. fuer Strahlentherapie; Adamietz, I.A. [Frankfurt Univ. (Germany). Abt. fuer Strahlentherapie; Rahn, R. [Zentrum der Zahn-, Mund- und Kieferheilkunde, Klinikum der Johann-Wolfgang-Goethe-Universitaet Frankfurt a.M. (Germany); Mose, S. [Frankfurt Univ. (Germany). Abt. fuer Strahlentherapie; Saran, F. [Frankfurt Univ. (Germany). Abt. fuer Strahlentherapie; Schopohl, B. [Frankfurt Univ. (Germany). Abt. fuer Strahlentherapie; Boettcher, H.D. [Frankfurt Univ. (Germany). Abt. fuer Strahlentherapie

    1995-08-01

    The degree of dosage increase in the immediate surrounding of metallic dental materials was measured in an in-vivo study during therapeutic irradiation with 60 Co gamma rays in the area of mouth cavity of 11 patients. Measurements were carried out by thermoluminescent dosimetry at permamently fixed golden teeth and alloy specimens containing gold and palladium and amalgam. The following relative dodage values according to a simultanelusly measured reference value were measured at the surface of the different dental materials: 161% near fixed golden caps, 168% near the specimen containing gold in a high percentage, 133% near the specimen of palladium and 161% near the specimen of amalgam. The in vivo measured dosage increases due to metallic dental prosthesis are less than values obtained using back scatter arramgements for irradiating phantoms. Despite this, they could be of clinical relevance. Thus the usage of a mucous membrane protection during irradiation with 60 Co, as a means of preventing local lesions of the oral mucosa, due to dental alloys within the treatment volume remains inevitable. (orig.) [Deutsch] In einer In-Vivo-Studie wurde thermolumineszenzdosimetrisch bei insgesamt elf Patienten, die im Bereich der Mundhoehle bestrahlt wurden, das Ausmass der Dosiserhoehung in unmittelbarer Umgebung dentaler metallischer Werkstoffe gemessen. Gemessen wurde an feststizenden Goldkronen, an Proben einer gold- und einer palladiumhaltigen Legierung und an Amalgam waehrend therapeutischer Bestrahlung mit Kobalt-60-Gammastrahlen. Folgende relativen Dosiswerte an der Oberflaeche der einzelnen zahnaertzlichen Materialien, bezogen auf einen simultan bestimmten Referenzwert, wurden bestimmt: an fixierten Goldkronen bis 161%, an der goldhaltigen Probe bis 168%, an der palladiumhaltigen bis 133% und an Amalgam bis 161%. Die in vivo gemessenen Dosiserhoehungen durch dentale metallische Werkstoffe sind geringer als die am Modell in Rueckstreugeometrie bei der zur Probe

  19. New epoxide polymers generated by metal organic catalysts (chelates) and their application in composite structures. Pt. 2. Final report; Neue Epoxidpolymere durch Metallorgano-Katalysatoren und ihr Einsatz in Faserverbundstrukturen. T. 2. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Merz, T.; Raeckers, B.

    1999-08-01

    Epoxy resins cured by catalysts based on chelate compound can offer an additional potential concerning storage and heat stability, toughness and curing characteristic. To prove these advantages and to prepare a serial application was target of the project, by which physico-chemical investigations, determination of mechanical properties and component tests were used. Besides an improvement of storage time and a minimized curing cycle a satisfactory processing behaviour and mechanical properties approaching currently used aircraft materials became evident. Serial production of fibre reinforced components for automotive industry is basically possible, because all requirements concerning costs, performance and station time were met. The results can also be used for new applications in other technical fields like apparatus construction or sporting goods industry. (orig.) [German] Mittels Metallorganokatalysatoren gehaertete Epoxidharze versprechen gegenueber herkoemmlichen Systemen zusaetzliche Potentiale hinsichtlich Lager- und Waermestabilitaet sowie Zaehigkeit und Haertungscharakteristik. Diese nachzuweisen und eine zukuenftige Serienanwendung vorzubereiten war Ziel des Projektes, wozu physico-chemische Untersuchungen, mechanische Kennwertermittlungen und Bauteilversuche dienten. Neben verbesserter Lagerstabilitaet und minimierten Haertungszyklen konnten gute Verarbeitungseingeschaften und ein Leistungsprofil nachgewiesen werden, das nahe an das heutiger Luftfahrtwerkstoffe heranreicht. Die Serienherstellung von Faserverbundbauteilen im Automobilbau ist prinzipiell moeglich, da durch die entwickelten Katalysatoren alle Vorgaben hinsichtlich Kosten, Leistungsfaehigkeit und Taktzeiten erreicht werden konnten. Hierdurch koennen sich auch fuer andere Bereiche (z.B. Geraetebau, Sportartikelindustrie) neue Anwendungsmoeglichkeiten eroeffnen. (orig.)

  20. The production of wear protection coatings reinforced with tungsten carbide by temperature-controlled welding with the CO{sub 2} laser; Herstellung wolframkarbidverstaerkter Verschleissschutzschichten durch temperaturgeregeltes Auftragschweissen mit dem CO{sub 2}-Laser

    Energy Technology Data Exchange (ETDEWEB)

    Nowotny, S.; Boddin, G.C.; Luft, A.; Techel, A. [Fraunhofer-Inst. fuer Werkstoffphysik und Schichttechnologie, Dresden (Germany); Uelze, A. [Hochschule fuer Technik und Wirtschaft Dresden (Germany)

    1995-12-31

    Protective coatings can be produced by laser coating with metal alloys reinforced by carbide, whose resistance to abrasive wear with a content of 40 to 50% by volume of hard material is comparable to sintered TC-Co hard metal. Due to the remaining ductile material behaviour and the metallurgical binding to the substrate, the coatings have high impact, fatigue and adhesion strengths. The use of process control leads to a stable coating process and to increased safety and reproduceability when working in narrow parameter areas. (orig./RHM) [Deutsch] Durch das Laserbeschichten mit karbidverstaerkten Metallegierungen koennen Schutzschichten erzeugt werden, deren Widerstand gegen Abrasivverschleiss bereits bei einem Hartstoff-Volumengehalt von 40 bis 50% gesintertem WC-Co-Hartmetall vergleichbar ist. Aufgrund des verbleibenden duktilen Werkstoffverhaltens und der metallurgischen Bindung zum Substrat verfuegen die Schichten ueber hohe Schlag-, Ermuedungs- und Haftfestigkeiten. Der Einsatz der Prozessregelung fuehrt zu einem stabilen Beschichtungsvorgang und zu einer erhoehten Sicherheit und Reproduzierbarkeit beim Arbeiten in eng begrenzten Parameterbereichen. (orig./RHM)

  1. Partizipative medienpädagogische Aktionsforschung. Methodologische Überlegungen anlässlich einer Untersuchung der Medienkompetenz von und durch SchülerInnen an Neuen Mittelschulen in Wien aus Sicht des Theorie-Praxis-Problems.

    Directory of Open Access Journals (Sweden)

    Christian Swertz

    2015-12-01

    Full Text Available Für die Medienpädagogik ist wie für jede Pädagogik das Theorie-Praxis-Problem relevant. Im Beitrag wird partizipative Aktionsforschung als Methode zur Gestaltung von Theorie-Praxis-Korrelationen untersucht. Dazu wird zunächst das Theorie-Praxis-Problem als Relation der Korrelationen zwischen Theorie und Praxis in Forschungs- und Unterrichtsmethoden bestimmt. Anschließend wird durch Interpretationen von Theorien zur partizipativen Aktionsforschung und von Daten aus einem Projekt untersucht, ob die Relation von Forschung und Unterricht so gestaltet werden kann, dass zu souveränen Akten in solidarisch-libertären Gemeinschaften aufgefordert wird. Die Ergebnisse zeigen, dass dies möglich und die Methode also medienpädagogisch relevant ist. As for any education, the theory-practice transformation problem is relevant for media education. In this paper, participatory action research is investigated as a method to design the correlation of theory and practice. To do so, the theory-practice problem is understood as the relation of the correlations between theory and practice in research and teaching methods. Subsequently, it is researched if the relation of research and teaching can be designed to call for sovereign acts in solidary-libertarian communities. The research is conducted by interpreting participatory action research theories and project data. It is shown that it is possible to call for sovereign acts. Thus the suggested method can be considered as relevant for media education.

  2. Cutting unemployment by a factor of 2 through energy efficiency and renewable energy use? Sustainable economic development for Switzerland in the 21st century; Halbierung der Arbeitslosigkeit durch Energieeffizienz und erneuerbare Energien? Nachhaltige Wirtschaftsentwicklung fuer die Schweiz im 21. Jahrhundert

    Energy Technology Data Exchange (ETDEWEB)

    Weizsaecker, E. von; Lehmann, H.; Meliss, M. [and others

    1999-07-01

    , andere Studien, die sich um weitere Aspekte der oben aufgefuehrten Fragen kuemmern, erlaeutert und die Potentiale der erneuerbaren Energietechnologien aufgezeigt. Die Studie untersucht ausschliesslich den Effekt der Einfuehrung des 'Solarrappens' in Hoehe von 0.005 CHF/kWh (Abgabe auf alle nicht erneuerbaren Energietraeger, welche gesamthaft ca. 880 Mio. CHF/Jahr liefern soll). Sie foerdert die Diskussion um die durch Energieeffizienz und erneuerbare Energien ausgeloesten Arbeitsplatzeffekte im ganzen Mitteleuropa. Fuer die Schweiz kommt die Studie zum Schluss, dass die vorgeschlagene Energieabgabe bei den ausgeloesten Investitionen eine Hebelwirkung von 5.5 bis 9.4 aufweist. Dementsprechend werden jaehrlich 60,000 bis 210,000 neue Arbeitsplaetze geschaffen, einen kleinen Teil davon in den Zulieferlaendern der Schweizer Wirtschaft. Dem gegenueber stehen Minderumsaetze und negative Beschaeftigungseffekte in der Mineraloel-, Gas- und Elektrizitaetswirtschaft. Per Saldo ergeben sich positive Beschaeftigungseffekte in der Hoehe von 63,000 Arbeitsplaetzen (Durchschnittswert mehrerer Szenarien). Es gibt dazu keine prinzipiellen technischen oder finanziellen Hindernisse, jedoch sind wesentliche Veraenderungen auf oekonomischer, rechtlicher und institutioneller Ebene noetig, auf denen Energiesysteme operieren. Die Studie entstand durch die Zusammenarbeit vom Bundesamt fuer Energie, einem unabhaengigen europaeischen Wissenschaftsinstitutes, mehreren Universitaeten, Vertretern innovativer Wirtschaftsverbaende und NGOs.

  3. Male gonadal dose an adjuvant 3-D-pelvic irradiation after anterior resection of rectal cancer. Influence to fertility; Radiogene Hodenbelastung durch Streustrahlung bei adjuvanter 3-D-Beckenbestrahlung nach anteriorer Resektion beim Rektumkarzinom. Einfluss auf die Fertilitaet

    Energy Technology Data Exchange (ETDEWEB)

    Piroth, M.D.; Hensley, F.; Wannenmacher, M.; Zierhut, D. [Radiologische Klinik der Univ. Heidelberg (Germany). Abt. fuer Klinische Radiologie

    2003-11-01

    Maenner erkranken. Wir untersuchten die radiogene Hodenbelastung durch Streustrahlung bei adjuvanter Beckenbestrahlung nach Operation eines Rektumkarzinoms. Die gemessenen Strahlendosen am Hoden wurden zu den Daten der Literatur in Beziehung gesetzt. Patienten und Methode: Wir fuehrten bei 18 Patienten mit thermolumineszenzdetektoren (TLD) In-vivo-Messungen zur Bestimmung der streustrahlenbedingten Gonadendosis durch. Die TLDs wurden an vier definierten Punkten des Skrotums fixiert. Die Bestrahlung erfolgte 3-D-geplant in Drei-Felder-Technik in Bauchlage im Lochbrett. Die Gesamtdosis betrug 50,4 Gy, die Einzeldosis 1,8 Gy taeglich. Nach 45 Gy erfolgte eine Modifikation der Bestrahlungstechnik auf opponierende und kranial verkuerzte Gegenfelder zur kompletten Duenndarmschonung. Ergebnisse: Die mittlere Gonadendosis aller 18 Patienten pro Bestrahlungsfraktion betrug 0,057 Gy (median 0,05 Gy), wobei die Mittelwerte der einzelnen Patienten zwischen 0,035 und 0,114 Gy lagen. Die Standardabweichung betrug 0,02 Gy. Nach 28 Fraktionen, 50,4 Gy entsprechend, errechnete sich kumulativ eine Gesamtdosis am Hoden von 1,60 Gy (0,98-3,19 Gy). Schlussfolgerungen: Das maennliche Keimepithel reagiert auf Bestrahlung ausserordentlich sensibel im Sinne eines negativen Fraktionierungseffektes. Bei Gesamtdosen > 1,5 Gy, fraktioniert appliziert, gilt die Azoospermie als meist irreversibel. Die von uns ermittelte Gonadendosis fuehrt nach den Daten der Literatur sehr wahrscheinlich zu einer dauerhaften Sterilitaet. Wegen der geringen Patientenanzahl sind die Daten vorsichtig zu interpretieren, dennoch muss der Patient ueber die hohe Wahrscheinlichkeit einer bleibenden Infertilitaet aufgeklaert werden. Eine moegliche praetherapeutische Spermakryokonservierung sollte mit dem Patienten besprochen werden. (orig.)

  4. Reduction of construction cost and carbon dioxide emissions by heat contracting. Obstacles and solutions. Final report; Baukostensenkung und CO{sub 2}-Minderung durch Waerme-Contracting. Hemmnisse und Loesungswege. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Ebert, V.; Liebernickel, T.; Froemel, T.; Kues, H.

    2000-11-01

    The poor condition of many heating systems is caused by a conflict of interest between investors and users. Landlords tended to opt for low investment cost, while the accompanying higher consumption cost was paid by their tenants. The only thing that has changed here is the fact that the 'secondary cost' has become a criterion for new tenants' decision to rent an apartment. Heat contracting may be a way out of the problem as contractors are interested in high-efficiency heating systems and optimally controlled operation. Heat contracting is a service concept of private and communal organisations that involves payment, construction and operation of a heating system by the contractor depending on the contracting model used. [German] Die relativ schlechte und veraltete heizungstechnische Ausruestung der Wohngebaeude in Deutschland ist auf den lange Jahre bestehenden Interessenkonflikt zwischen Investor und Nutzer im Mietwohnungsbau zurueckzufuehren. Durch die Umlagefaehigkeit der Heizkosten auf die Nutzer (Mieter) war der Gebaeudeeigentuemer i.d.R. bestrebt, eine Anlage zu guenstigen Investitionskosten zu erwerben. Die daraus resultierenden, relativ hoeheren Verbrauchskosten waren als Betriebskosten auf die Mieter umlegbar und belasteten den Vermieter nicht. An dieser Situation hat sich bis heute prinzipiell nichts geaendert; allerdings sind die Nebenkosten mit der Hauptkostenposition 'Heizkosten' zu einem Kriterium der Vermietbarkeit geworden, so dass der Vermieter heute die Kosten moeglichst niedrig halten muss. Ein Ausweg aus dem Interessenkonflikt und der neuen Vermietsituation koennte das Waerme-Contracting sein. Seitens des Contractors ist der Einsatz hocheffizienter Anlagentechnik und optimale, d.h. ueberwachte Betriebsbedingungen fuer einen wirtschaftlichen Erfolg unabdingbar. Waerme-Contracting ist ein Dienstleistungskonzept von privatwirtschaftlichen und kommunalen Unternehmen, die - je nach Contracting

  5. Engine 3E. NO{sub x} reduction by means of homogenisation of mixtures inside combustion chambers. Final report; Engine 3E. NO{sub x}-Reduktion durch Homogenisierung des Gemisches in Brennkammern. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Zarzalis, N.; Homann; Schubert

    1999-11-05

    This is the final report of the Technology Project 'NO{sub x} reduction by means of homogenisation of mixtures inside combustion chambers', which is part of the Engine 3E 2010 programme of the BMBF, which was to promote research in aircraft engineering. In this project, technologies were developed to achieve a 60% reduction of NO{sub x} emissions as compared to ICAO '95 in an annular combustion chamber in realistic operating conditions where the emissions of substances oher than NO{sub x} were to be of the same level as the emissions of modern jet drives. Further, the design of the new combustion chamber was to be conventional in order to retain the possibility of converting existing propulsion systems to the new technology. [German] Diese Notiz enthaelt den Abschlussbericht des Technologievorhabens 'NO{sub x}-Reduktion durch Homogenisierung des Gemisches in Brennkammern'. Das Vorhaben ist Teil des Luftfahrtfoerderprogramms Engine 3E 2010 der Bundesregierung und wird vom Bundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie (BMBF) unter dem Foerderkennzeichen 20T9540 gefoerdert. Das Programm wurde am 01. Juli 1995 begonnen und endete am 30. Juni 1999. Entsprechend dem Foerderantrag wurden in dem Vorhaben Einzeltechnologien erarbeitet, die erforderlich sind, um eine 60% Reduktion der NO{sub x}-Emissionen gegenueber ICAO '95 in einer Ringbrennkammer unter realistischen Betriebsbedingungen zu demonstrieren, wobei die restlichen Emissionen das Emissionsniveau moderner Triebwerke nicht ueberschreiten sollten. Darueber hinaus sollte das aeussere Design der zu entwickelnden Brennkammer sind von konventionellen Brennkammern nicht unterscheiden, um die Moeglichkeit der Umruestung von alten Triebwerken mit der Brennkammern der neuen Technologie offen zu halten. (orig.)

  6. Minimally invasive treatment of abcesses by CT-guided drainage with a basket catheter system; Minimal invasive Behandlung von Abszessen durch CT-gesteuerte Drainage mit einem Korbkatheter-System

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, R.D.; Henrich, H.; Buddenbrock, B.; Barkhausen, J.; Langer, R. [Universitaetsklinikum Essen (Germany). Inst. fuer Roentgendiagnostik OZ II; Erhard, J. [Universitaetsklinikum Essen (Germany). Abt. fuer Allgemeine Chirurgie

    1997-09-01

    58 patients with abscesses in different locations and origins have been treated by an interventional radiologic procedure. On the whole 77 basket-katheters were placed under CT-guidance into abscess formations of different size and localization. 36 patients developed an abscess after surgery, two patients archieved abscess drainage after embolisation of a tumor. In 20 patients the abscess was a complication of a septic infectious disease. Open surgery was avoided in 41/58 patients of these patients 9/41 received only percutaneous drainage and 32/41 patients suffering from illness were given antibiotic medication according to the resistogram in combination with percutaneous drainage. 17/58 ptients required secondary surgery but with a lower risk because of the smaller abscess volume and the better clinical constitution after percutaneous drainage. A minimally invasive management of abscesses using a basket-catheter system is successful even in localisations deep inside the body and hard to reach. An irreversible catheter occlusion followed by the implantation of a new catheter system could be avoided in all patients. (orig.) [Deutsch] 58 Patienten, mit abszedierenden Entzuendungen am Koerperstamm wurden radiologisch interventionell durch Einbringen von insgesamt 77 Korbkathetern versorgt. 36mal war der Abszess eine postoperative Komplikation, 2mal wurde eine Abszessdrainage nach einer Tumorembolisation erforderlich. Bei weiteren 20 Patienten waren keine chirurgischen Massnahmen vorausgegangen. 41/58 der Patienten wurden ohne operativen Eingriff geheilt, davon fuehrte 9mal die alleinige Drainage zum Erfolg, bei den uebrigen 32 Patienten bestand die erfolgreiche Behandlung aus Drainage in Kombination mit einer spezifischen systemischen Antibiose nach Resistenzbestimmung. 17/58 Patienten wurden sekundaer operiert. Das Einbringen und die Entfernung eines grosslumigen Korbkathetersystems ist auch bei tiefliegenden, schwer zugaenglichen Abszessen problemlos moeglich

  7. Reduction of construction cost and carbon dioxide emissions by heat contracting. Obstacles and solutions. Final report; Baukostensenkung und CO{sub 2}-Minderung durch Waerme-Contracting. Hemmnisse und Loesungswege. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Ebert, V.; Liebernickel, T.; Froemel, T.; Kues, H.

    2000-11-01

    The poor condition of many heating systems is caused by a conflict of interest between investors and users. Landlords tended to opt for low investment cost, while the accompanying higher consumption cost was paid by their tenants. The only thing that has changed here is the fact that the 'secondary cost' has become a criterion for new tenants' decision to rent an apartment. Heat contracting may be a way out of the problem as contractors are interested in high-efficiency heating systems and optimally controlled operation. Heat contracting is a service concept of private and communal organisations that involves payment, construction and operation of a heating system by the contractor depending on the contracting model used. [German] Die relativ schlechte und veraltete heizungstechnische Ausruestung der Wohngebaeude in Deutschland ist auf den lange Jahre bestehenden Interessenkonflikt zwischen Investor und Nutzer im Mietwohnungsbau zurueckzufuehren. Durch die Umlagefaehigkeit der Heizkosten auf die Nutzer (Mieter) war der Gebaeudeeigentuemer i.d.R. bestrebt, eine Anlage zu guenstigen Investitionskosten zu erwerben. Die daraus resultierenden, relativ hoeheren Verbrauchskosten waren als Betriebskosten auf die Mieter umlegbar und belasteten den Vermieter nicht. An dieser Situation hat sich bis heute prinzipiell nichts geaendert; allerdings sind die Nebenkosten mit der Hauptkostenposition 'Heizkosten' zu einem Kriterium der Vermietbarkeit geworden, so dass der Vermieter heute die Kosten moeglichst niedrig halten muss. Ein Ausweg aus dem Interessenkonflikt und der neuen Vermietsituation koennte das Waerme-Contracting sein. Seitens des Contractors ist der Einsatz hocheffizienter Anlagentechnik und optimale, d.h. ueberwachte Betriebsbedingungen fuer einen wirtschaftlichen Erfolg unabdingbar. Waerme-Contracting ist ein Dienstleistungskonzept von privatwirtschaftlichen und kommunalen Unternehmen, die - je nach Contracting

  8. Separation of uranium from aqueous solutions using calix[6]arenes in liquid-liquid extraction as well as solid phase extraction; Abtrennung von Uran aus waessriger Loesung durch Calix[6]arene mittels Fluessig-Fluessig-Extraktion sowie Festphasen-Extraktion

    Energy Technology Data Exchange (ETDEWEB)

    Schmeide, K.; Geipel, G.; Bernhard, G.

    2004-11-01

    The suitability of different calyx[n] arene types for uranyl extraction from liquid solutions was examined by means of liquid-liquid extraction using aqueous phases and organic solvents of varying compositions. It was found that COOH-derivatised calyx[6] arenes have good extraction properties and can even be used in the acid pH range. The use of calixarene-modified fleeces for the separation of uranyl from aqueous phases was examined in batch experiments with pH and uranyl concentration as variables and in the presence or absence of competing ions. The results showed that calixarene-modified fleeces can be used for uranium separation starting from pH 4. At pH 5, up to a maximum of 7.6 x 10{sup -7} mol uranium can be bound per 1 g of calixarene-modified fleece. The separation of uranyl from synthetic pit waters was examined as a means of testing the separation capacity of calixarene-modified fleeces in environmentally sensitive waters. Studies on the reversibility of uranium bonding to calixarene-treated polyester fleeces have shown that under environmentally realistic conditions (neutral pH range) the uranium is firmly bound to the calixarene-modified fleeces and cannot be mobilised. By contrast, in acidic environments calixarene-modified fleeces are capable of near-complete regeneration. Such regenerated textile filter materials can then be used for further uranium separation cycles. [German] Die Eignung verschiedener Calix[n]aren-Typen fuer die Uranylextraktion aus waessriger Loesung wurde mittels Fluessig-Fluessig-Extraktion unter Variation der Zusammensetzung der waessrigen Phase und des organischen Loesungsmittels untersucht. Es konnte gezeigt werden, dass COOH-derivatisierte Calix[6]arene gute Extraktionseigenschaften aufweisen und selbst im sauren pH-Bereich angewendet werden koennen. Die Uranylabtrennung aus waessriger Loesung durch calixarenmodifizierte Vliese wurde in Abhaengigkeit des pH-Wertes und der Uranylkonzentration in Abwesenheit und Gegenwart von

  9. Durch Kooperation und Vernetzung zu neuen Aufgabenfeldern – Serviceentwicklung in der Bibliothek der Medizinischen Fakultät Mannheim [Opening up new tasks through cooperation and networking – service development in the Library for the Medical Faculty of Mannheim

    Directory of Open Access Journals (Sweden)

    Semmler-Schmetz, Martina

    2013-09-01

    Full Text Available [english] The Library for the Medical Faculty of Mannheim of the University of Heidelberg supplies literature and information for research, teaching and further education purposes at the “Universitätsmedizin Mannheim”. Cooperation and networking with the central facilities, departments, working groups, and information multipliers result in new, future-oriented areas of work that go beyond traditional library tasks. New services for students focus primarily on academic research and writing skills, while information and consulting services which are flexible in terms of time and location are being developed for staff members of the clinical departments and institutes. The recent cooperation with actors in the field of evidence-based medicine has yielded the innovative service “systematic literature search” which is designed to support doctors and scientists in the preparation of grant proposals for clinical trials, systematic reviews, and guidelines. Currently, the library is widening access to its new services to include doctors, scientists, and other health practitioners in the region, and evaluating chargeable services for these user groups.[german] Die Bibliothek der Medizinischen Fakultät Mannheim der Universität Heidelberg dient der Literatur- und Informationsversorgung für Forschung, Lehre, Fort- und Weiterbildung an der Universitätsmedizin Mannheim. Neue, zukunftsorientierte Aufgabenfelder, die über die klassischen bibliothekarischen Arbeitsschwerpunkte hinausgehen, erschließt sich die Bibliothek durch enge Kooperation und Vernetzung mit den zentralen Einrichtungen und Abteilungen, Arbeitskreisen und Multiplikatoren innerhalb der Universitätsmedizin Mannheim. Neue Services für die Zielgruppe der Studierenden richten sich vor allem auf den Bereich des wissenschaftlichen Arbeitens, während für Mitarbeiter in den Einrichtungen, Kliniken und Instituten der Universitätsmedizin zeitlich und räumlich flexible Informations

  10. Optimised utilisation of existing incinerators by installation of upstream reactors for treatment of waste with high calorifica value - HYBRID waste treatment plants; Optimierte Nutzung bestehender Abfallverbrennungsanlagen durch Errichtung vorgeschalteter Reaktoren zur Behandlung heizwertreicher Abfaelle - HYBRID-Abfallbehandlungsanlagen

    Energy Technology Data Exchange (ETDEWEB)

    El Labani, M.

    2000-07-01

    Abfallverbrennungsanlagen nachtraeglich um leistungsfaehige Rauchgasreinigungssysteme ergaenzt werden. Dies fuehrte zu einem erheblichen Anstieg der Kosten und Behandlungsgebuehren mit dem entsprechenden Druck zur optimierten Nutzung. Zusaetzliche Behandlungskapazitaeten werden benoetigt fuer heizwertreiche Abfaelle. Sie koennen zwar in konventionellen Abfallverbrennungsanlagen fuer Hausmuell behandelt werden, bedingen aber aufgrund des hoeheren Heizwertes eine Durchsatzreduzierung was die Kostensituation weiter negativ beeinflusst. Daher favorisieren aktuelle Konzepte die Behandlung heizwertreicher Abfaelle in spezifischen, dezentralen Anlagen. Die ueblicherweise kleinen Anlagen gehen einher mit hohen Nebenkosten der Errichtung. Zur Abschaetzung des Optimierungspotentials bestehender Abfallverbrennungsanlagen wurde deren Auslastungssituation untersucht. Bei umfangreichen Messkampagnen am MHKW Darmstadt konnte selbst bei 100%iger Auslastung der Verbrennungseinheit in der Rauchgasreinigungsanlage eine Luecke aufgezeigt werden, die durch eine entsprechend angepasste Vorschaltanlage aufgefuellt werden koennte. Eine solche Vorschaltanlage definiert zusammen mit der bestehenden Abfallverbrennungsanlage eine HYBRID-Abfallbehandlungsanlage. Die Bereitstellung eines Instrumentariums zur Unterstuetzung der Entscheidungsfindung bei der Konzeption solcher Anlagen ist Gegenstand dieser Arbeit. (orig.)

  11. Verhaltenstherapeutische Angstbehandlung durch Strategische Kurzzeittherapie (SKT

    Directory of Open Access Journals (Sweden)

    Hauke G

    2008-01-01

    Full Text Available Die besondere Stärke verhaltenstherapeutischer Angstbehandlung in der Praxis liegt in ihren erprobten Methoden für eine zügige Symptomreduktion. Daneben richten neuere Entwicklungen der Verhaltenstherapie zusätzlich ihre Aufmerksamkeit auf funktionelle Zusammenhänge hinter der Symptomatik. Sie entwickelten sich aus der Erfahrung, dass eine reine Symptombehandlung oft nicht ausreicht, um ein stabiles Behandlungsergebnis zu erzielen. Strategische Kurzzeittherapie bündelt diese Entwicklungen zu einem stringenten Behandlungsansatz. Unter Berücksichtigung primärer und sekundärer Emotionen lassen sich Reaktionsketten konzipieren, die ein tieferes Verständnis der Angstsymptomatik ermöglichen. Die Therapiekonzeption erarbeitet dabei kognitiv-affektive Schemata der Patienten, so genannte Überlebensregeln, die in entscheidender Weise die Entwicklung von zielführenden Therapiestrategien definieren.

  12. Health injuries by noise; Gesundheitsgefahren durch Laerm

    Energy Technology Data Exchange (ETDEWEB)

    Plath, P. [Klinik fuer Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie, Bochum Univ., Prosper-Hospital, Recklinghausen (Germany)

    1995-07-01

    Within the civilized world, noise is a common danger for health. There is not only the well known hearing loss by occupational noise, but there are also a lot of noise induced hearing losses by noise injuries in private life which are comparable to the occupational ones. The sources of these injuries within private life mostly are unknown and can be found in household goods as well as from listening to loud music. Also the danger from loud cracks mostly is underestimated, so as the cracks from children toys often reach more than 150 dB(Al). Alladay`s noise levels, e.g. in public trafic, also reach dangerous levels, and the sum of all these noise injuries results in a lesion of the cilias of the outer hair cells in the Corti`s organ. The resulting hearing loss is called `sociacusis` and increases the effect of physiological presbyacusis to an amount that results in the fact, that more than 10% of all adults in civilized countries have a severe, handicapping deafness. The immediate effect of noise in this regard is intensified by the effects of disturbing noise onto the psychovegetative and hormonal regulations by chronical stress. So people in civilized countries are going to become a society of hard hearing subjects, and deafness will become a common peoples` disease. (orig.) [Deutsch] Laerm ist in der zivilisierten Welt ein allgemeines Gesundheitsproblem. Es gibt nicht nur die berufliche Laermschwerhoerigkeit, die gut erforscht ist, sondern schaedlicher Laerm tritt sehr oft auch im Privatleben auf und verursacht Gehoerschaeden, die der beruflich bedingten Laermschwerhoerigkeit vergleichbar sind. Die Gehoergefaehrdung im Privatleben ist oft unbekannt und betrifft neben lauten Geraeten im Haushalt und dem Hoeren zu lauter Musik auch die oft in ihrer Gefaehrlichkeit unterschaetzten Knalle, die selbst bei Kinderspielzeug Spitzenwerte der Pegel von ueber 150 dB(Al) erreichen. Die allgemeine zivilisatorische Laermbelastung, z.B. im oeffentlichen Verkehr, die oft schaedigende Ausmasse erreicht, im Beurteilungspegel aber unter den Grenzwerten bleibt, bewirkt im Laufe der Jahre ebenfalls bleibende Schaeden an der Zilien der aeusseren Haarzellen des Corti-Organs, die eine ueber die physiologische Alterung hinausgehende `Soziakusis` bewirken. Schliesslich haben die staendigen Geraeusch- und Laermbelaestigungen und -stoerungen des Alltagslebens deutliche Effekte auf die neurovegetativen und hormonellen Regulationen des Organismus und verursachen somit mittelbar ueber Stressreaktionen ebenfalls Hoerstoerungen in Form von Hoerstuerzen und Tinnitus. Der alltaegliche Laerm, dem zu wenig Bedeutung zugemessen wird, droht Schwerhoerigkeit, von der mehr als 10% aller Erwachsenen betroffen sind, zur Volkskrankheit werden zu lassen. (orig.)

  13. Modulation der GABAergen Wirkung durch Lebensmittelinhaltsstoffe

    OpenAIRE

    2016-01-01

    Lebensmittel bzw. Lebensmittelinhaltsstoffe können die neuronale Aktivität beeinflussen und somit eine neurophysiologische Wirkung auslösen. γ-Aminobuttersäure (GABA), bekannt als der wichtigste inhibitorische Neurotransmitter, spielt eine wichtige Rolle bei der Regulation der Aktivität der exzitatorischen Neurotransmission. Aus diesem Grund ist das GABAerge System von großer Bedeutung für die Behandlung von neurophysiologischen Störungen und ein Target für verschiedene Medikamentengruppen, u...

  14. Legitimation allein durch Entwicklung? : Das Beispiel Ruanda

    OpenAIRE

    Stroh, Alexander

    2007-01-01

    "In Ruanda soll 2008 zum zweiten Mal nach dem Genozid (1994) ein Teil des Parlaments gewählt werden. Eine Debatte um die Bedeutung der Qualität dieser Wahlen für die weitere Entwicklungszusammenarbeit ist noch nicht erkennbar. Obgleich sich global ein allgemeiner Zusammenhang zwischen Demokratie und Entwicklungszuwendungen etabliert hat, wird die autokratische Herrschaft in Ruanda von Geberseite akzeptiert. Das Land erhält gegenwärtig so viel Entwicklungshilfe wie nie zuvor in Friedenszeiten....

  15. Internationale Wachstumsstrategien produzierender Familienunternehmen durch Mergers & Acquisitions

    NARCIS (Netherlands)

    Sachs, Andreas Fabian Constantin Johannes

    2007-01-01

    Family businesses are experiencing a revival in the German economy. In the face of the challenges of globalization and the ongoing relocation of production facilities, “home base oriented” enterprises meet with increasing attention and gain in importance. Family-owned enterprises have indeed been ne

  16. Characteristics of purple nonsulfur bacteria grown under Stevia residue extractions.

    Science.gov (United States)

    Xu, J; Feng, Y; Wang, Y; Lin, X

    2013-11-01

    As a consequence of the large-scale cultivation of Stevia plants, releases of plant residues, the byproduct after sweetener extraction, to the environment are inevitable. Stevia residue and its effluent after batching up contain large amounts of organic matters with small molecular weight, which therefore are a potential pollution source. Meanwhile, they are favourite substrates for micro-organism growths. This investigation was aimed to utilize the simulated effluent of Stevia residue to enrich the representative purple nonsulfur bacterium (PNSB), Rhodopseudomonas palustris (Rps. palustris), which has important economic values. The growth profile and quality of Rps. palustris were characterized by spectrophotometry, compared to those grown in common PNSB mineral synthetic medium. Our results revealed that the simulated effluent of Stevia residue not only stimulated Rps. palustris growth to a greater extent, but also increased its physiologically active cytochrome concentrations and excreted indole-3-acetic acid (IAA) content. This variation in phenotype of Rps. palustris could result from the shift in its genotype, further revealed by the repetitive sequence-based PCR (rep-PCR) fingerprinting analysis. Our results showed that the effluent of Stevia residue was a promising substrate for microbial growth.

  17. „Wir verbrachten mehr als 24 Stunden, ohne etwas anderes als Schokolade und Limonade zu uns zu nehmen“. Hinweise in Alexander von Humboldts Tagebuchaufzeichnungen zu Fragen der Verpflegung auf der Forschungsreise durch Spanisch-Amerika

    Directory of Open Access Journals (Sweden)

    Ursula Thiemer-Sachse

    2013-12-01

    Full Text Available Zusammenfassung In Alexander von Humboldts Tagebüchern seiner berühmten Forschungsreise durch Spanisch-Amerika finden sich Bemerkungen und Hinweise für künftige Leser seiner Reisedarstellungen zu Problemen der Verpflegung. Zumeist werden Engpässe und Missstände erwähnt sowie Fragen soziopolitischer Situation verdeutlicht. Sie beziehen sich auf Extrembedingungen in stadtfernen Gebieten und im Kontakt mit den indigenen Trägern und Helfern während der einzelnen Exkursionen. Die Darstellungen der alltäglichen Gegebenheiten sind zugleich eine interessante Quelle für Verhaltensmuster der verschiedenen Schichten der spanischkolonialen Gesellschaft wie der Forschungsreisenden, die mit den Schwierigkeiten umzugehen hatten, sich anzupassen verstanden oder ihre eigenen Verhaltensmuster entwickelt haben. Damit ermöglichen Humboldts Aufzeichnungen zugleich auch auf diesem Gebiet manchen Blick auf den seither erfolgten Kulturwandel. Resumen En los diarios del famoso viaje de estudios de Alejandro de Humboldt por la América española se encuentran observaciones y notas sobre problemas de aprovisionamiento, en favor de lectores futuros de sus relaciones de viaje. En la mayoría de los casos Humboldt menciona situaciones precarias o ilustra cuestiones de carácter sociopolítico. Frecuentemente, sus apuntes se refieren a condiciones extremas en regiones remotas y en contacto con los cargadores y ayudantes indígenas durante las diferentes excursiones. Las descripciones de los acontecimientos cotidianos al mismo tiempo son una fuente interesante sobre los modos de proceder de las diferentes capas de la sociedad colonial española así como de los viajeros científicos que tuvieron que enfrentar dificultades a las cuales había que adaptarse o desarrollar modelos propios de comportamiento. Por esto al mismo tiempo las noticias de Humboldt también en este asunto posibilitan más de una vez la atención al cambio cultural que se ha realizado hasta hoy

  18. Recall dermatitis caused by reexposition of decotaxel following irradiation of the brain. Case report and review of literature; Recall-Dermatitis durch Docetaxel-Reexposition nach Gehirnbestrahlung. Fallbeobachtung und Literaturuebersicht

    Energy Technology Data Exchange (ETDEWEB)

    Giesel, B.U.; Kutz, G.G.; Thiel, H.J. [Klinikum Bamberg (Germany). Klinik fuer Strahlentherapie und Radioonkologie

    2001-09-01

    -Hals-Region integriert. Hierbei muss gerade in der palliativen Situation einer Verstaerkung der Nebenwirkungen durch Interaktion der einzelnen Therapiekomponenten besondere Aufmerksamkeit geschenkt werden. Wir berichten deshalb ueber eine schwere Recall-Dermatitis, die wir bei zwei Patientinnen mit einem Mammakarzinom nach Gehirnbestrahlung und anschliessender Reexposition mit Docetaxel beobachtet haben. Patienten und Methode: Von Januar bis Maerz 1999 wurden an unserer Klinik zwei Patientinnen mit bereits fortgeschritten metastasiertem Mammakarzinom wegen neu aufgetretener Gehirnmetastasierung bzw. Meningeosis carcinomatosa einer Ganzhirnbestrahlung unterzogen (2 Gy fuenfmal woechentlich bis zu einer Referenzdosis von 50 Gy). Beide Patientinnen wurden seit Oktober bzw. November 1998 mehrfach mit Docetaxel (Taxotere {sup cirledR}) 30 mg/m{sup 2} Koerperoberflaeche (KOF) woechentlich bzw. 100 mg/m{sup 2} KOF monatlich behandelt. Nach Abschluss der Bestrahlung wurde die Docetaxel-Behandlung fortgesetzt. Ergebnisse: Von beiden Patientinnen wurde Docetaxel vor und waehrend der Bestrahlung gut vertragen. Erst nach Abschluss der Bestrahlung und Docetaxel-Reexposition kam es reproduzierbar zu einem massiven Erythem im Bereich der gesamten bestrahlten Kopfhaut mit Ausbildung feuchter Epitheliolysen und Krustenbildung (CTC-Grad IV). Schlussfolgerung: Die in unseren beiden Faellen beobachtete Dermatitis nach Bestrahlung und Docetaxel-Reexposition ist Ausdruck eines Recall-Phaenomens. Der zugrunde liegende Pathomechanismus ist derzeit noch nicht vollstaendig geklaert. Es bleibt zu untersuchen, inwieweit die Taxane mit der Strahlentherapie - auch im Hinblick auf zukuenftige primaere Radiochemotherapiekonzepte - kombiniert werden koennen, ohne schwerwiegende Toxizitaeten im Bereich der strahlenexponierten Haut oder Schleimhaut auszuloesen. (orig.)

  19. Fundamentals for the development of individual risk assessment by UVB irradiation. Final report; Grundlagen zur Entwicklung einer personenbezogenen Risikoabschaetzung durch UV-B-Strahlung. T. A: Exogene Biomarker zur UV-Personendosimetrie. T. B: Mechanismen der UV-Suszeptibilitaet. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Horneck, G.; Rettberg, P.; Scherer, K.; Scharfetter-Kochanek, K.; Wlaschek, M.; Schneider, L.

    2002-07-01

    -sensitiven Bacillus subtilis-Sporen erwies sich als besonders geeignet, die UV-Belastung einzelner Individuen zu dokumentieren. Der DLR-Biofilm, der die Schaedigung der DNA nach UVA und UVB-Exposition biologisch wichtend erfasst, wurde parallel mit dem elektronischen UV-Breitbandmessgeraet X2000 weiterentwickelt. Es zeigte sich, dass die Farbaenderungen in der Haut sich nicht stufenfoermig, sondern trotz einer Dosisabhaengigkeit kontinuierlich ausbilden. Die Personendosimetrie fuehrte zur Bestimmung und Korrelation biologisch gewichteter effektiver Dosisbereiche der klinischen Parameter MED, IPD und PPD. Die Identifizierung einer in vitro fuer diverse Biomarker dosisabhaengig ablaufenden Zellantwort liess sich nicht generell in das komplexe in vivo System der Haut uebertragen. Es konnte herausgearbeitet werden, dass es moeglich ist, in der Praxis UV-Expositionsdosen unbekannter Groesse ueber den DLR-Biofilm direkt und unmittelbar in relative Einheiten eines biologisch erzeugten Schadens in vivo zu uebersetzen. Mit diesen Arbeiten ist eine personenbezogene in vivo Dosiserfassung und Korrelation der UV-Effekte in der Pathogenese von Hauttumorentstehung und -ausbreitung durch UV-Strahlung zumindest partiell moeglich geworden. (orig.)

  20. Der Einfluss von Anonymität in der Lehrevaluation durch Studierende [The Effects of Anonymity on Student Ratings of Teaching and Course Quality in a Bachelor Degree Programme

    Directory of Open Access Journals (Sweden)

    Schaffner, Noemi

    2013-08-01

    is being taken seriously; consequently, they feel able to provide a constructive and honest evaluation.[german] Zielsetzung und Fragestellung: Unterscheiden sich die Ergebnisse anonymisierter und personalisierter Evaluationen? Methodik: Während zweier Jahre wurden bei jeder Modulevaluation alle Studierenden randomisiert den Halbgruppen „anonym“ und „personalisiert“ zugeteilt. Die Qualität des Moduls in seinen relevanten Aspekten wurde mit einem standardisierten Fragebogen erhoben. Zusätzlich konnten optionale Textantworten formuliert werden. Drei unabhängige Personen bewerteten die Aussagequalität der Antworten gemäss einem Leitfaden. Dieser beinhaltete die fünf Dimensionen positiv-negativ, differenziert-absolut, Nennung einer Person-allgemein, Befehl enthaltend-neutral und optisch akzentuiert-blank. Der Datensatz bestand aus 615 Fragebogen, davon waren 306 in anonymer Form. Mit einer multivariaten Varianzanalyse wurde überprüft, ob sich zwischen anonym und personalisiert erhobenen Daten ein Unterschied sowohl bei den durch Skalen bewerteten Aspekten als auch bei der Qualität der optionalen Textantworten zeigte. Zusätzlich wurde untersucht, ob sich die Häufigkeit der optionalen Textantworten unterschied.Ergebnisse: In der Aussagequalität liess sich kein signifikanter Unterschied zwischen anonym und personalisiert erhobenen Daten nachweisen. Hingegen haben Studierende aus der personalisierten Gruppe tendenziell häufiger optionale Textantworten geliefert.Schlussfolgerung: Personalisierte Evaluationen generieren dann keine verfälschten Resultate im Sinne der sozialen Erwünschtheit, wenn der Evaluationszirkel geschlossen und transparent ist: Die Verantwortlichen melden den Studierenden die Evaluationsergebnisse konsequent zurück und Änderungs-, Optimierungswünsche und Umsetzungsmöglichkeiten werden mit ihnen diskutiert. Die Studierenden erfahren so, dass ihre Rückmeldungen ernst genommen werden. So können sie sich echte Kritik erlauben.

  1. Redirection of metabolism for hydrogen production

    Energy Technology Data Exchange (ETDEWEB)

    Harwood, Caroline S.

    2011-11-28

    This project is to develop and apply techniques in metabolic engineering to improve the biocatalytic potential of the bacterium Rhodopseudomonas palustris for nitrogenase-catalyzed hydrogen gas production. R. palustris, is an ideal platform to develop as a biocatalyst for hydrogen gas production because it is an extremely versatile microbe that produces copious amounts of hydrogen by drawing on abundant natural resources of sunlight and biomass. Anoxygenic photosynthetic bacteria, such as R. palustris, generate hydrogen and ammonia during a process known as biological nitrogen fixation. This reaction is catalyzed by the enzyme nitrogenase and normally consumes nitrogen gas, ATP and electrons. The applied use of nitrogenase for hydrogen production is attractive because hydrogen is an obligatory product of this enzyme and is formed as the only product when nitrogen gas is not supplied. Our challenge is to understand the systems biology of R. palustris sufficiently well to be able to engineer cells to produce hydrogen continuously, as fast as possible and with as high a conversion efficiency as possible of light and electron donating substrates. For many experiments we started with a strain of R. palustris that produces hydrogen constitutively under all growth conditions. We then identified metabolic pathways and enzymes important for removal of electrons from electron-donating organic compounds and for their delivery to nitrogenase in whole R. palustris cells. For this we developed and applied improved techniques in 13C metabolic flux analysis. We identified reactions that are important for generating electrons for nitrogenase and that are yield-limiting for hydrogen production. We then increased hydrogen production by blocking alternative electron-utilizing metabolic pathways by mutagenesis. In addition we found that use of non-growing cells as biocatalysts for hydrogen gas production is an attractive option, because cells divert all resources away from growth and

  2. The orientation of the primary donor in bacterial photosynthesis.

    Science.gov (United States)

    Frank, H A; Friesner, R; Nairn, J A; Dismukes, G C; Sauer, K

    1979-09-11

    The triplet state EPR spectra of magnetically aligned whole cells of Rhodopseudomonas viridis and Rhodopseudomonas palustris display a marked dependence on the orientation of the static EPR field with respect to the alignment field direction. This observation implies that the primary donor species on which the triplets are localized are ordered within the membranes. We have developed a theoretical model for the system to enable calculation of the orientation of the magnetic axes of the primary donor species with respect to the membranes in which they reside. The triplet state spectra are generated by an ensemble of partially ordered magnetic systems and a computer simulation of the experimental results. The triplet orientation is very similar for the two organisms studied, where one axis lies predominantly in the plane of the membrane and the other two axes have approximately equal projections onto the normal to the membrane.

  3. Characterization of clouds and aerosols by lidar remote sensing with regard to the transfer of UV radiation. Final report; Leitthema 4: Solare UV-B-Strahlung. Teilvorhaben: Charakterisierung von Wolken und Aerosolen durch Lidar-Fernerkundung hinsichtlich des UV-Strahlungstransfers. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Jaeger, H.; Muecke, R.; Kreipl, S.

    2000-05-01

    The investigations characterize aerosols and clouds over Garmisch-Partenkirchen by lidar remote sensing, and support the interpretation of measurements of the transfer of UV radiation. Extensive tests of calibration procedures were performed, and the lidar system was modified to meet the requirements. The focal point were measurements accompanying the CUVRA (Characteristics of the UV Radiation field in the Alps) campaign at IFU in March 1999. 767 lidar measurements in two wavelength channels were performed on 12 days during this campaign. Aerosol and cloud situations were characterized by determining lower and upper layer heights (time resolution down to 1 minute, height resolution down to 15 meters), multi layer situations from ground to the tropopause region (up to 5 aerosol and cloud layers), optical depths at 532 nm and 355 nm (range 0.01 to 3), wavelength dependences of the optical depth between 532 nm and 355 nm (range of wavelength exponent 0 to -2), and lidar ratios (extinction/backscatter) at 532 nm (range 20 to 38). (orig.) [German] Die Untersuchungen charakterisieren Aerosole und Wolken ueber Garmisch-Partenkirchen durch Lidar-Fernerkundung und dienen der Interpretation von Messungen des UV-Strahlungsflusses. Hierzu waren umfangreiche Arbeiten zum Testen von Eichverfahren notwendig, ebenso wie Modifizerungen des Lidarsystems. Schwerpunkt der Untersuchungen waren Messungen waehrend der CUVRA (Characteristics of the UV Radiation field in the Alps) Messkampagne im Maerz 1999 am IFU. Waehrend dieser Kampagne wurden an 12 Messtagen 767 Lidarmessungen in jeweils zwei Wellenlaengenkanaelen durchgefuehrt. Die Aerosol- und Wolkensituationen wurden charakterisiert durch die Bestimmung von Schichtunter- und obergrenzen (Zeitaufloesung bis zu 1 Minute, Hoehenaufloesung bis zu 15 m), der Mehrschichtigkeit vom Boden bis zum Tropopausenbereich (bis zu 5 Aerosol- und Wolkenschichten), der optischen Dicken bei 532 nm und 355 nm (Bereich 0.01 bis 3), der

  4. GenBank blastx search result: AK060364 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK060364 001-008-H11 U75363.1 Rhodopseudomonas palustris cyclohex-1-ene-1-carboxyl-CoA hydratase (bad...k), cyclohex-1-ene-1-carboxylate CoA ligase (aliA), cyclohexanecarboxyl-CoA dehydrogenase (bad...J), 2-ketocyclohexanecarboxyl-CoA hydrolase (badI), putative 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (bad...H), putative regulatory protein (badR), putative NADPH:quinone oxidoreductase (bad...C), and benzoyl-CoA reductase subunits (badD), (badE), (badF) and (badG) genes, complete cds.|BCT BCT 8e-11 +3 ...

  5. GenBank blastx search result: AK243161 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243161 J100034I22 U75363.1 RPU75363 Rhodopseudomonas palustris cyclohex-1-ene-1-c...arboxyl-CoA hydratase (badk), cyclohex-1-ene-1-carboxylate CoA ligase (aliA), cyclohexanecarboxyl-CoA dehydrogenase (bad...J), 2-ketocyclohexanecarboxyl-CoA hydrolase (badI), putative 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (bad...H), putative regulatory protein (badR), putative NADPH:quinone oxidoreductase (bad...C), and benzoyl-CoA reductase subunits (badD), (badE), (badF) and (badG) genes, complete cds. BCT 3e-40 1 ...

  6. GenBank blastx search result: AK061856 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK061856 001-040-G03 U75363.1 Rhodopseudomonas palustris cyclohex-1-ene-1-carboxyl-CoA hydratase (bad...k), cyclohex-1-ene-1-carboxylate CoA ligase (aliA), cyclohexanecarboxyl-CoA dehydrogenase (bad...J), 2-ketocyclohexanecarboxyl-CoA hydrolase (badI), putative 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (bad...H), putative regulatory protein (badR), putative NADPH:quinone oxidoreductase (bad...C), and benzoyl-CoA reductase subunits (badD), (badE), (badF) and (badG) genes, complete cds.|BCT BCT 2e-25 +2 ...

  7. GenBank blastx search result: AK242840 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242840 J090070E16 U75363.1 RPU75363 Rhodopseudomonas palustris cyclohex-1-ene-1-c...arboxyl-CoA hydratase (badk), cyclohex-1-ene-1-carboxylate CoA ligase (aliA), cyclohexanecarboxyl-CoA dehydrogenase (bad...J), 2-ketocyclohexanecarboxyl-CoA hydrolase (badI), putative 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (bad...H), putative regulatory protein (badR), putative NADPH:quinone oxidoreductase (bad...C), and benzoyl-CoA reductase subunits (badD), (badE), (badF) and (badG) genes, complete cds. BCT 5e-24 1 ...

  8. GenBank blastx search result: AK105359 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK105359 001-119-C12 U75363.1 Rhodopseudomonas palustris cyclohex-1-ene-1-carboxyl-CoA hydratase (bad...k), cyclohex-1-ene-1-carboxylate CoA ligase (aliA), cyclohexanecarboxyl-CoA dehydrogenase (bad...J), 2-ketocyclohexanecarboxyl-CoA hydrolase (badI), putative 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (bad...H), putative regulatory protein (badR), putative NADPH:quinone oxidoreductase (bad...C), and benzoyl-CoA reductase subunits (badD), (badE), (badF) and (badG) genes, complete cds.|BCT BCT 4e-15 +2 ...

  9. GenBank blastx search result: AK243680 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243680 J100090I20 U75363.1 RPU75363 Rhodopseudomonas palustris cyclohex-1-ene-1-c...arboxyl-CoA hydratase (badk), cyclohex-1-ene-1-carboxylate CoA ligase (aliA), cyclohexanecarboxyl-CoA dehydrogenase (bad...J), 2-ketocyclohexanecarboxyl-CoA hydrolase (badI), putative 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (bad...H), putative regulatory protein (badR), putative NADPH:quinone oxidoreductase (bad...C), and benzoyl-CoA reductase subunits (badD), (badE), (badF) and (badG) genes, complete cds. BCT 9e-20 1 ...

  10. Fluorescence enhancement of light-harvesting complex 2 from purple bacteria coupled to spherical gold nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Bujak, Ł. [Nicolaus Copernicus Univ., Torun (Poland). Inst. of Physics; Czechowski, N. [Nicolaus Copernicus Univ., Torun (Poland). Inst. of Physics; Piatkowski, D. [Nicolaus Copernicus Univ., Torun (Poland). Inst. of Physics; Litvin, R. [Nicolaus Copernicus Univ., Torun (Poland). Inst. of Physics; Mackowski, S. [Nicolaus Copernicus Univ., Torun (Poland). Inst. of Physics; Brotosudarmo, T. H. P. [Ma Chung Univ., Malang (Indonesia). Ma Chung Research Center for Photosynthetic Pigments; Pichler, S. [Univ. of Glasgow, Scotland (United Kingdom). Inst. of Molecular, Cell and Systems Biology; Cogdell, R. J. [Univ. Linz (Austria). Inst. fur Halbleiter-und Festkorperphysik; Heiss, W. [Univ. Linz (Austria). Inst. fur Halbleiter-und Festkorperphysik

    2011-10-24

    The influence of plasmon excitations in spherical gold nanoparticles on the optical properties of a light-harvesting complex 2 (LH2) from the purple bacteria Rhodopseudomonas palustris has been studied. Systematic analysis is facilitated by controlling the thickness of a silica layer between Au nanoparticles and LH2 complexes. Fluorescence of LH2 complexes features substantial increase when these complexes are separated by 12 nm from the gold nanoparticles. At shorter distances, non-radiative quenching leads to a decrease of fluorescence emission. The enhancement of fluorescence originates predominantly from an increase of absorption of pigments comprising the LH2 complex.

  11. DNA-directed spatial assembly of photosynthetic light-harvesting proteins.

    Science.gov (United States)

    Henry, Sarah L; Withers, Jamie M; Singh, Ishwar; Cooper, Jonathan M; Clark, Alasdair W; Burley, Glenn A; Cogdell, Richard J

    2016-01-28

    This manuscript describes the surface immobilization of a light-harvesting complex to prescribed locations directed by the sequence-selective recognition of duplex DNA. An engineered light-harvesting complex (RC-LH1) derived from Rhodopseudomonas (Rps.) palustris containing the zinc finger (ZF) domain zif268 was prepared. The zif268 domain directed the binding of zfRC-LH1 to target double-stranded DNA sequences both in solution and when immobilized on lithographically defined micro-patterns. Excitation energy transfer from the carotenoids to the bacteriochlorophyll pigments within zfRC-LH1 confirmed that the functional and structural integrity of the complex is retained after surface immobilization.

  12. Carbon Dynamics of Pinus palustris Ecosystems Following Drought

    Directory of Open Access Journals (Sweden)

    Gregory Starr

    2016-04-01

    Full Text Available Drought can affect forest structure and function at various spatial and temporal scales. Forest response and recovery from drought may be a result of position within landscape. Longleaf pine forests in the United States have been observed to reduce their carbon sequestration capacity during drought. We collected eddy covariance data at the ends of an edaphic longleaf pine gradient (xeric and mesic sites over seven years; two years of normal rainfall were followed by 2.5 years of drought, then 2.5 years of normal or slightly above-average rainfall. Drought played a significant role in reducing the physiological capacity of the sites and was compounded when prescribed fire occurred during the same periods. The mesic site has a 40% greater basal area then the xeric site, which accounts for its larger sequestration capacity; however, both sites show the same range of variance in fluxes over the course of the study. Following drought, both sites became carbon sinks. However, the xeric site had a longer carry-over effect and never returned to pre-drought function. Although this study encompassed seven years, we argue that longer studies with greater spatial variance must be undertaken to develop a more comprehensive understanding of forest response to changing climate.

  13. Posttranslational modification of a vanadium nitrogenase.

    Science.gov (United States)

    Heiniger, Erin K; Harwood, Caroline S

    2015-08-01

    In microbes that fix nitrogen, nitrogenase catalyzes the conversion of N2 to ammonia in an ATP-demanding reaction. To help conserve energy some bacteria inhibit nitrogenase activity upon exposure to ammonium. The purple nonsulfur phototrophic bacterium Rhodopseudomonas palustris strain CGA009 can synthesize three functional nitrogenase isoenzymes: a molybdenum nitrogenase, a vanadium nitrogenase, and an iron nitrogenase. Previous studies showed that in some alphaproteobacteria, including R. palustris, molybdenum nitrogenase activity is inhibited by ADP-ribosylation when cells are exposed to ammonium. Some iron nitrogenases are also posttranslationally modified. However, the posttranslational modification of vanadium nitrogenase has not been reported. Here, we investigated the regulation of the alternative nitrogenases of R. palustris and determined that both its vanadium nitrogenase and its iron nitrogenase activities were inhibited and posttranslationally modified when cells are exposed to ammonium. Vanadium nitrogenase is not found in all strains of R. palustris, suggesting that it may have been acquired by horizontal gene transfer. Also, phylogenetic analyses of the three nitrogenases suggest that VnfH, the target of ADP-ribosylation, may be the product of a gene duplication of nifH, the molybdenum nitrogenase homolog.

  14. Investigations of the acceleration of microbial degradation of PAH in soils with high PAH contamination by coffein in practical conditions. Final report; Untersuchungen zur Beschleunigung des mikrobiellen Abbaus von polycyclischen aromatischen Kohlenwasserstoffen in Boeden mit hoher PAH-Kontamination durch Coffein unter Praxisbedingungen. Anhang. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Grimmer, G.; Collins, H.J.

    1998-01-01

    fAH degradation by Mucor circinelloides was proved several times in the course of this project. Although PAH contamination could never be completely removed, a number of parameters were identified that influence successful PAH degradation, e.g. soil homogeneity, oxygen supply to the microorganisms, pH, and nutrient supply. The effect of the complexing agent coffein on PAH degradation, which had been described already in preliminary investigations, was validated several times. Optimisation experiments showed that the pre-determined optimum coffein concentration of 0.3% in the nutrient broth provided the best results also in the present investigations. There was no suggestion of a negative effect of various cations on the growth of Mucor circinelloides which may be mobilized in the soils by the acid nutrient broth. A comparison of residual PAH concentrations after microbial treatment in the model soil ``tar-pitch-doped quartz sand`` and the soil ``gas works`` showed that different residual concentrations must be expected depending on the soil material. This volume presents a summary of the results. (orig./SR) [Deutsch] Im Rahmen dieses Projektes konnte der Abbau von PAH durch Mucor circinelloides mehrfach nachgewiesen werden. Es wurde in keinem Fall eine vollstaendige Entfernung der PAH-Kontamination erreicht, jedoch stellten sich eine Reihe von Parametern als wesentlich fuer einen erfolgreichen PAH-Abbau heraus (Homogenitaet des Bodenmaterials, Versorgung der Mikroorganisme mit Sauerstoff, pH-Wert, Naehrstoffversorgung). Der bereits in Voruntersuchungen beschriebene Effekt des Komplexbildners Coffein auf den PAH-Abbau wurde bei den durchgefuehrten Untersuchungen mehrfach bestaetigt. Optimierungsexperimente fuehrten zu dem Ergebnis, dass die bereits als optimal ermittelte Coffeinkonzentration von 0,3% im Naehrmedium auch fuer die hier untersuchten Boeden die besten Ergebnisse liefert. Ein negativer Einfluss verschiedener Kationen auf das Wachstum von Mucor

  15. Wireless energy transmission through the abdominal wall for operating implanted actuators and recharging implanted energy storages with due consideration to the simultaneous undisturbed transcutaneous transmission of encoded signals for controlling various functions of the implant; Drahtloser Energietransfer durch die Bauchdecke zum Betrieb von implantierten Aktoren und zur Wiederaufladung von implantierten Energiespeichern unter Beruecksichtigung der gleichzeitigen ungestoerten transkutanen Uebertragung von codierten Signalen zur Steuerung verschiedener Funktionen eines Implantats

    Energy Technology Data Exchange (ETDEWEB)

    Peter, S.

    2007-07-01

    integrierten Energiespeicher damit geladen werden koennen, ohne Energieleitungen durch die Bauchdecke hindurch fuehren zu muessen. Dieses ziel wurde durch die Entwicklung und Anwendung eines auf die Anforderungen im urologischen Bereich eingesetzten medicinischen Implantats abgestimmten induktiven und damit transkutanen Energietransfer realisiert. Dazu wurde unter anderem ein umfangreiches Simulationsmodell zur Berechnung mit der Finiten-Element-Methode erstellt. Als Resultat ergeben sich eine Reihe von Vorteilen fuer die betroffenen Patienten: vollstaendige Vermeidung von Infektionen die sonst durch eine staendig offene ''Wunde'' in der Bauchdecke, bedingt durch die hindurch tretende Energieleitung, vorhanden waere; hierdurch ergibt sich wiederum der Vorteil, dass die Patienten weniger Medikamente zu sich nehmen muessen und die Kosten fuer eine staendige Wundbehandlung entfallen; weitestgehende Unabhaengigkeit von der stationaeren Energieversorgung im Betrieb des Systems (ueber mehrere Tage keine Notwendigkeit staendig eine Netzsteckdose in der Naehe haben zu muessen), damit geht eine wesentlich gesteigerte Mobilitaet der Patienten einher. Die Entwicklung einer transkutanen Signaluebertragung mit Hilfe von Infrarot bzw. RF Technologie hat die Rueckgewinnung einer willentlichen Steuerung der Miktion erst entscheidend ermoeglicht und beseitigt damit eine der Hauptaengste von betroffenen Patienten, ein fremdbestimmtes und durch ein von kurzen festgelegten Intervallen wiederkehrender Entleerungstaetigkeit (rund um die Uhr) gepraegtes Leben fuehren zu muessen. Die zusaetzliche Abfragemoeglichkeit des Reservoirfuellstandes ermoeglicht dabei eine Kontrolle zu beliebigen Zeitpunkten und fuehrt deshalb zu einer entscheidend verbesserten Planbarkeit der Toilettengaenge. Dies stellte das zweite Ziel dar. Die Kombination des transkutanen Energie- und Signaltransfers fuehrte u.a. zu einem Gesamtsystem, das die Lebensqualitaet der von einer Totaloperation betroffenen

  16. Development and comparison of membrane methods for improving the performance of municipal waster water purification by combination of activation biology and microfiltration and construction and optimisation of an experimental plant; Entwicklung und Vergleich von Membranverfahren zur Leistungssteigerung der kommunalen Abwasserreinigung durch die Kombination von Belebungsbiologie und Mikrofiltration sowie Bau und Optimierung einer technischen Versuchsanlage

    Energy Technology Data Exchange (ETDEWEB)

    Finner, M.

    2003-12-17

    The goal of the present project was to develop a membrane-based activation method, optimise it on an experimental plant and operate this plant alongside a conventional municipal water treatment plant over a one-year period. An important part of the project consisted in making a technical comparison between conventional water purification and the membrane-based activation method. The goal was to optimise the membrane-based activation method to a point where its use in place of conventional methods based on activation biology could be considered promising also in economic terms. [German] In dem Projekt war es vorgesehen, ein Membranbelebungsverfahren zu entwickeln, durch den Betrieb einer technischen Anlage zu optimieren und diese parallel zu einer konventionellen kommunalen Klaeranlage ueber einen Zeitraum von einem Jahr zu betreiben. Ein wesentlicher Projektbestandteil war der technische Vergleich der konventionellen Abwasserreinigung mit dem Membranbelebungsverfahren. Ziel war es, das Membranbelebungsverfahren technisch soweit zu optimieren, dass sein Einsatz als Alternative zu konventionellen Belebungsbiologien kuenftig auch unter wirtschaftlichen Bedingungen als erfolgversprechend gelten kann. (orig.)

  17. Risks and opportunities of the liberalized electricity market. Partial project: BoFiT, an integrated decision support system for retaining the competitiveness of the cogeneration technology. Final report; Risiken und Chancen des liberalisierten Strommarktes. Teilprojekt: Integrierte Entscheidungsunterstuetzung durch BoFiT zur Erhaltung der Wettbewerbsfaehigkeit der Kraft-Waerme-Kopplung. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Stock, G.; Scheidt, M.

    2002-06-04

    The energy management system called BoFiT and specific new applications are explained which have been developed as one project task of the coordinated research project sponsored by the German ministry of economics,(BMWi), entitled ''Risks and opportunities of the liberalized electricity market''. The major objective of the project is: Finding efficient strategies to ensure the competitiveness of the cogeneration technology in the deregulated power and gas markets, in particular for the purpose of enhancing the penetration of the ecologically beneficial, cogenerated district heating supply in those markets. The focus of this report is on a specific application of the integrated BoFiT decision support system, for which the ''model for microsimulation of spot transactions at the Power Exchange based on a multi-agent system'' has been developed and is explained in great detail. (orig./CB) [German] Mit dem Energiemanagementsystem BoFiT soll den Unternehmen eine effektive und operativ nutzbare Entscheidungsunterstuetzung angeboten werden. Wesentliche Ziele des Verbundprojektes des BMWi sind: Unterstuetzung der KWK und speziell der darauf basierenden oekologisch sinnvollen Fernwaermeversorgung durch Anpassung von Arbeitsablaeufen und Werkzeugen zur Findung betriebswirtschaftlich optimaler Einsatz- und Betriebsstrategien; Kostenoptimierung der Kraftwerke, Vertraege und Stromhandelsaktivitaeten unter den Randbedingungen der deregulierten Strom- und Gasmaerkte. Als Beispiel einer solchen integrierten Entscheidungsunterstuetzung wird das ''Modell zur Mikrosimulation des Spothandels von Strom auf der Basis eines Multi-Agenten-Systems'' ausfuehrlich beschrieben. (orig./CB)

  18. Possible risk and probability of causation of bone and liver cancer due to the occupational alpha-ray exposure of workers at the previous WISMUT Uranium Mining Company; Moegliches Risiko und Verursachungs-Wahrscheinlichkeit von Knochen-und Leberkrebs durch die berufliche Alphastrahlen-Exposition von Beschaeftigten der ehemaligen WISMUT-AG

    Energy Technology Data Exchange (ETDEWEB)

    Jacobi, W.; Roth, P. [GSF - Forschungszentrum fuer Umwelt und Gesundheit Neuherberg GmbH, Oberschleissheim (Germany). Inst. fuer Strahlenschutz; Nosske, D. [Bundesamt fuer Strahlenschutz, Neuherberg (Germany). Inst. fuer Strahlenhygiene

    1998-03-01

    moegliche Risikobeitrg diskutiert, der durch das Trinken von radioaktivem Grubenquellwaessern verursacht sein koennte. Die resultierenden Risikowerte pro Einheit der Exposition sind im Anhang in Abhaengigkeit von der Zeit nach Exposition aufgelistet. Beispielhaft werden fuer Referenzwerte der Exposition von Untertage-Bergarbeitern das zu erwartende zusaetzliche absolute und relative Strahlenrisiko sowie die daraus folgende Verursachungs-Wahrscheinlichkeit in Abhaengigkeit vom Alter bei Exposition und vom Alter bei Krebsinzidenz dargestellt. In Bezug auf die Latenzzeitverteilung zeigen die Ergebnisse, dass ein konstantes relatives Risikomodell - wie es von UNSCEAR fuer solide Tumore auf Grund der epidemiologischen Daten von den Atombomben-Ueberlebenden abgeleitet wird - im Falle von Knochen- und Leberkrebs durch inkorporierte, alpha-strahlende Radionuklide nicht zutreffend ist. - Abschliessend wird empfohlen, die im vorliegenden Bericht entwickelten Risikomodelle bei der Entscheidung ueber die Anerkennung von Knochen- und Leberkrebs als strahlenbedingte Berufskrankheit (BK Nr. 2402) bei WISMUT-Beschaeftigten anzuwenden. (orig./GL)

  19. A fast method for measurement and clearance of building rubble containing {alpha}-emitting radionuclides: Direct {alpha} spectroscopy at thin, large-surface measuring sources - automation of the method. Final report; Schnelles Freimessverfahren fuer alpha-aktive Nuklide in Bauschutt durch Direktmessung von grossflaechigen duennen Messpraeparaten - Automatisierung des Verfahrens. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Nebelung, C.; Barz, B. [Forschungszentrum Rossendorf e.V. (FZR), Dresden (Germany); Henninger, J.; Dang, T.H. [Technische Univ. Dresden (Germany)

    2001-08-01

    . (orig.) [German] Beim Abriss von Nuklearanlagen fallen grosse Mengen Bauschutt an, die mit Radionukliden kontaminiert sein koennen. Da diese Kontaminationen haeufig nahe der Freigabewerte liegen, ist fuer die Entscheidung, ob das Material als radioaktiver Abfall behandelt werden muss oder freigegeben werden kann, eine schnelle und exakte Messmethode notwendig. Eine direkte Messung der {alpha}-aktiven Nuklide im stehenden Gebaeude ist auf Grund der geringen Reichweite der {alpha}-Strahlung und ihrer Absorption im Beton nicht moeglich. Die uebliche chemische Analyse der {alpha}-aktiven Nuklide ist sehr arbeits- und zeitaufwendig und somit ungeeignet. Im Rahmen dieses Projektes wurde die Methode der direkten {alpha}-Spektrometrie nach nur mechanischer Praeparation des Betons bis zur technischen Anwendungsreife weiterentwickelt. In einem aus zwei Schritten bestehenden Zerkleinerungsprozess wurden Suspensionen mit mittleren Partikeldurchmessern von 0,5 {mu}m hergestellt. Die Messproben mit einem Durchmesser von 20 cm und Schichtdicken zwischen 1 und 2,5 {mu}m wurden durch Spruehen auf die Probentraeger und anschliessendes Trocknen hergestellt. Fuer die Stabilitaet der Suspensionen wurden Peptisatoren und Tenside getestet, die agglomeratfreie Suspensionen und damit gleichmaessige Schichten garantieren. Die Spektren dieser Proben wurden in einer Gitterionisationskammer (GIK) aufgenommen. Es wurden Untersuchungen zur Verringerung des Nulleffektes der GIK durchgefuehrt, um die Nachweisgrenze der Methode zu verringern. Die Spektrenauswertung erfolgte durch Peakfitting mit einer der Peakform angepassten kombinierten Gaussschen und exponentiellen Funktion. Eine zweite Methode zur Spektrenanalyse ist das verbesserte Programm WINKRUM, basierend auf Strahlungstransportgerechnungen. Dieses Modell beruecksichtigt Partikelgroessenverteilung und Packungsdichte, spezifische Dichte, Schichtdicke, Parameter der GIK und die Energie der Actiniden. Mit dieser Direktmessung duenner Messproben

  20. Quantification of the magnetization-transfer contrast effect: can it yield additional information in differentiation of musculoskeletal lesions particularly in separation of benign from malignant lesions; Quantifizierung des Magnetization Transfer Contrast (MTC) Effektes durch Berechnung von MT-Quotienten: Ergeben sich Zusatzinformationen fuer die Differenzierung benigner und maligner Erkrankungen des Bewegungsapparates?

    Energy Technology Data Exchange (ETDEWEB)

    Vahlensieck, M.; Traeber, F.; Schild, H. [Radiologische Universitaetsklinik Bonn (Germany); Gieseke, J. [Philips Medizinsysteme (Germany)

    1999-12-01

    Purpose: To investigate the potential information of the amount of magnetization-transfer effect in musculoskeletal lesions and to compare MT ratios from benign and malignant musculoskeletal lesions. Material and Method: 49 patients with malignant tumors (3 osteosarcoma, 3 malignant fibrous histiocytoma, 4 chondrosarcoma, 2 Ewing sarcomas) and benign lesions (8 chondroma, 2 fibrous dysplasia, 3 osteoid-osteoma, 6 ganglion cyst, 3 cyst, 3 osteomyelitis, 4 tendinitis, 3 rotator cuff tear, 5 scar tissue) were scanned using routine MRI protocols including T{sub 1}- and T{sub 2}-weighted spin echo as well as T{sub 2}*-weighted gradient echo (FFE) sequences at 1.5 Tesla (ACS II, Philips Medical). Additionally MTC images were generated by combining the FFE sequence and the off-resonance MT technique (-1500 Hz off-resonance frequency, 1770 flip angle and 50 ms pulse duration). MT ratios were calculated as SI{sub o}-SI{sub m}/SI{sub o}. Results: The MT ratio of benign lesions was 26{+-}15%, that of malignant lesions was 22{+-}6%. The difference was statistically not significant. As expected muscle showed a high MT ratio of 50{+-}8%. Scar tissue demonstrated an MT ratio of 39{+-}16% which was significantly higher than the tumor MT ratios. Conclusion: MTC (MT ratios) failed to show significant differences between benign and malignant lesions as was expected due to basic differences in cellularity, rate of mitosis and chromatin content. MTC might however gain more importance in separating scar tissue from recurrent tumor in the future. (orig.) [German] Zielsetzung: Durch die Quantifizierung des Magnetization Transfer Contrastes sollte untersucht werden, ob sich Zusatzinformationen in der Magnetresonanztomographie des Stuetz- und Bewegungsapparates ergeben. Insbesondere sollte ermittelt werden, ob gut- und boesartige Laesionen unterschiedliche MT-Quotienten aufweisen. Material und Methode: 49 Patienten mit boesartigen Tumoren (3 Osteosarkom, 4 Chondrosarkom, 3 Malignes

  1. Comparative analysis of the toxic effects of natural toxins and harmful substances produced by conventional processing methods or by irradiation and of toxicity tests; Vergleichende Darstellung der toxischen Wirkung nativer sowie durch herkoemmliche Verarbeitungsmethoden oder Strahlenbehandlung entstandener Schadstoffe in Lebensmitteln und Methoden ihrer toxikologischen Pruefung

    Energy Technology Data Exchange (ETDEWEB)

    Dahlhelm, H.; Arndt, K.; Groeger, G.; Schreiber, G.A.; Boegl, K.W.

    1994-12-31

    Aufschlussverfahren und Extraktkonsmethoden fuer komplexe Lebensmittel dargestellt, die bei der toxikologischen Pruefung von Nahrungsmittlen in in-vito-Systemen verwendet wurden. Die durch Strahlenbehandlung von Lebensmitteln und Lebensmittelbestandteilen gebildeten Radiolyseprodukte und die Ergebnisse ihrer Toxizitaetspruefung werden behandelt. Lebensmittel, die mit Strahlendosen bis zu 10 kGy behandelt werden, gelten als toxikologisch sicher. Eine Uebersicht der toxikologisch geprueften bestrahlten Lebensmittel sowie der dabei applizierten Maximaldosen liefern Tabellen am Ende des Kapitels 8. Unter den toxikoligischen Untersuchungen an bestrahlten Lebensmitteln finden vor allem jene Erwaehnung, die Anlass zu Diskussionen ueber gesundheitlich bedenkliche Effekte so behandelter Produkte gegeben haben. Daneben wird auch auf die Schwierigkeiten der Toxizitaetspruefung strahlenbehandelter Lebensmittel im Fuetterungsezperiment eingegangen. Die verwendeten Kurzzeittests zur Ermittlung der Genotoxizitaet strahlenbehandelter Lebensmittel und wesentliche Ergebnisse der toxikologischen Pruefung sind in Tabellen dargestellt. Ein Ueberblick zum Vorkommen, der Haeufigkeit und den gesundheitlichen Risiken nativer Toxine und durch herkoemmliche Zubereitungs- und Konservierungsmethoden erzeugter Schadstoffe in Lebensmitteln soll den Vergleich mit eventuellen gesundheitlichen Gefahren strahlenbehandelter Lebensmittel ermoeglichen. Im Schlusskapitel wird die Aussagekraft von Tierexperimenten und von in-vitro-Untersuchungen fuer die Voraussage der toxischen Wirkung schaedlicher Substanzen in Lebensmitteln beim Menschen diskutiert. (VHE)

  2. Investigations into the acceleration of microbial degradation of PAH in soils with high PAH contamination by coffein in practical conditions. Central analytical procedure and microbial effects; Untersuchungen zur Beschleunigung des mikrobiellen Abbaus von polycyclischen aromatischen Kohlenwasserstoffen in Boeden mit hoher PAH-Kontamination durch Coffein unter Praxisbedingungen. Teilprojekt A und B. Zentrale Analytik und mikrobiologische Begleituntersuchungen. Entwicklung einer optimalen Anwendungstechnik sowie oekonomische Evaluierung in Relation zu bestehenden Verfahren. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Grimmer, G.; Collins, H.J.; Muennich, K.

    1998-01-01

    PAH degradation by Mucor circinelloides was proved several times in the course of this project. Although PAH contamination could never be completely removed, a number of parameters were identified that influence successful PAH degradation, e.g. soil homogeneity, oxygen supply to the microorganisms, pH, and nutrient supply. The effect of the complexing agent coffein on PAH degradation, which had been described already in preliminary investigations, was validated several times. Optimisation experiments showed that the pre-determined optimum coffein concentration of 0.3% in the nutrient broth provided the best results also in the present investigation. There was no suggestion of a negative effect of various cations on the growth of Mucor circinelloides which may be mobilized in the soils by the acid nutrient broth. (orig./SR) [Deutsch] Im Rahmen dieses Projektes konnte der Abbau von PAH durch Mucor circinelloides mehrfach nachgewiesen werden. Es wurde in keinem Fall eine vollstaendige Entfernung der PAH-Kontamination erreicht, jedoch stellten sich eine Reihe von Parametern als wesentlich fuer einen erfolgreichen PAH-Abbau heraus (Homogenitaet des Bodenmaterials, Versorgung der Mikroorganisme mit Sauerstoff, pH-Wert, Naehrstoffversorgung). Der bereits in Voruntersuchungen beschriebene Effekt des Komplexbildners Coffein auf den PAH-Abbau wurde bei den durchgefuehrten Untersuchungen mehrfach bestaetigt. Optimierungsexperimente fuehrten zu dem Ergebnis, dass die bereits als optimal ermittelte Coffeinkonzentration von 0,3% im Naehrmedium auch fuer die hier untersuchten Boeden die besten Ergebnisse liefert. Ein negativer Einfluss verschiedener Kationen auf das Wachstum von Mucor circinelloides, die durch das saure Naehrmedium eventuell in den Boeden mobilisiert werde, war nicht festzustellen. Der Vergleich der PAH-Restgehalte nach dem mikrobiellen PAH-Abbau bei dem Modellboden `teerpechdotierter Quarzsand` und dem Boden `Gaswerk` zeigt, dass je nach untersuchtem

  3. High-productivity laser deposition welding using powder and wire. Final report of the project 'Higher efficiency of thermal coating plants by a combination of energy sources'; Hochproduktives Laser-Auftragschweissen mit Pulver und Draht. Ergebnisbericht zum Verbundvorhaben ''Wirkungsgradsteigerung thermischer Beschichtungsanlagen durch Energiequellenkombination''. SPRAYNERGY

    Energy Technology Data Exchange (ETDEWEB)

    Nowotny, Steffen (ed.)

    2013-05-01

    Excellent precision, maximum mechanical strength and selective properties of the generated coatings and 3D structures caused a breakthrough of laser deposition welding in industrial production. However, the technology is limited by its low deposition rates in those cases where large-surface components are to be coated while laser-typical corrosion and wear resistance characteristics are to be achieved. Examples are, e.g. hydraulic cylinders for offshore applications, tools for petroleum or gas production and mining, or large-scale conveyors. In these cases, modern laser hybrid technologies open up new applications. By effectively combining the laser energy with other energy sources, e.g. inductive heating, autogenic flames or electric resistance, high deposition rates are achieved with excellent coating qualities, which makes the laser process competitive also in the case of large-surface coatings. (orig./AKB) [German] Exzellente Praezision, hoechste mechanische Festigkeit und massgeschneiderte Eigenschaften von Oberflaechenschichten wie auch generativ erzeugten 3D-Strukturen: diese Merkmale haben dem Auftragschweissen mit Laserstrahlen zum Durchbruch in der industriellen Produktion verholfen. Die geringen Auftragraten setzen der Technologie jedoch in jenen Faellen Grenzen, wo es um Flaechenbeschichtungen grosser Bauteile geht, deren Einsatz aber dennoch die lasertypischen Schichteigenschaften hinsichtlich Korrosions- oder Verschleissbestaendigkeit erfordert. Beispiele sind lange Hydraulikzylinder im Offshore-Bereich, Werkzeuge der Oel- und Gasfoerderung und des Bergbaus sowie grosse Foerderschnecken. Hier erweitern moderne Laser-Hybridtechnologien wirkungsvoll die Einsatzmoeglichkeiten. Durch effiziente Kombination der Laserenergie mit anderen Energiequellen in Form induktiver Erwaermung, autogener Flammen oder elektrischem Widerstand werden hohe Auftragraten bei gleichzeitigem Erhalt der besonderen Schichtqualitaet erreicht, die das Laserverfahren auch bei

  4. Reduction of heating energy demand of residential buildings and of gas emission relevant for the greenhouse effect by using sand-lime building material in order to avoid thermal bridges in brick wall construction. Final report; Reduzierung des Heizenergiebedarfs von Wohngebaeuden und der Emissionen treibhausrelevanter Gase durch Einsatz von Kalksand-Spezialbaustoffen zur Vermeidung von Waermebruecken im Mauerwerksbau. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Eden, W. [Forschungsvereinigung ' Kalk-Sand' e.V., Hannover (Germany); Buhl, J.C. [Hannover Univ. (Germany). Inst. fuer Mineralogie

    2001-11-01

    The yearly energy consumption in the Federal Republic of Germany in the field of space heating still amounts to 30% of the yearly overall energy consumption. The sand-lime brick industry has the opportunity to make a contribution to energy conservation by avoiding too high heat flows over thermal bridges. Detailed constructional solutions do not always suffice to minimise the effects of thermal bridges. The use of bricks with low thermal conductivity and simultaneous high stone pressure resistance is useful for all detailed solutions. The influence of environmentally friendly, available and technically perfect lightweight aggregates for the production of sand-lime bricks has been investigated systematically and in detail in the frame of this research project. The main target of the project is to guarantee the manufacturing of bricks with high norm pressure resistance and simultaneous low thermal conductivity. This research target has been reached. (orig.) [German] Der jaehrliche Energieverbrauch in der Bundesrepublik Deutschland fuer den Sektor Raumwaerme betraegt immer noch ca. 30% vom jaehrlichen Gesamtenergieverbrauch. Fuer die Kalksandsteinindustrie besteht die Moeglichkeit, einen Beitrag zur Energieeinsparung durch Vermeidung von erhoehten Waermestroemen ueber Waermebruecken zu leisten. Nicht immer sind rein konstruktive Detailloesungen ausreichend, um die Auswirkung von Waermebruecken zu minimieren. Bei allen Detailpunkten ist der Einsatz von Mauersteinen mit einer niedrigen Waermeleitfaehigkeit und gleichzeitig einer hohen Steindruckfestigkeit sinnvoll. Mit dem Forschungsvorhaben wurde zum einen der Einfluss umweltfreundlicher, verfuegbarer und technisch einwandfreier Leichtzuschlaege fuer die Herstellung von Kalksand-Leichtsteinen systematisch und detailliert untersucht. Hauptziel dieses Projektes ist es, Mauersteine mit einer hohen Normdruckfestigkeit bei gleichzeitig niedriger Waermeleitfaehigkeit sicher herzustellen. Das Forschungsziel wurde erreicht

  5. Harmful loading of water courses caused by precipitation. Phase 2. Part project 8: Loading of harmful substances by precipitation. Pt. A, B, and C. Pt. A: Harmful substances introduced by atmospheric deposition dust.Pt. B: Sources and mobility of harmful substances. Pt. C: Load of harmful substances from roof and road surfaces; NIEDERschlagsbedingte SCHadstoffbeLAstung der Gewaesser - Phase 2. Teilprojekt 8: Schadstoffbelastung durch Niederschlag. T. A, B und C. T. A: Schadstoffeintrag durch atmosphaerische Staubdeposition. T. B: Quellen und Mobilitaet von Schadstoffen. T. C: Schadstofffrachten von Dach- und Strassenflaechen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Luetzner, K.; Grothkopp, H.; Gebhard, V.; Rennert, S.; Huth, B.

    1997-02-28

    Stadtgebieten ist die Belastung der Oberflaechenabfluesse nicht allein von der Verkehrsdichte abhaengig. Dies gilt hoechstens ueber die Partikelbeladung hinsichtlich einiger Elemente. Das Fahrverhalten, z.B. haeufiges Anfahren und Bremsen, ist hier ebenso von Bedeutung. Weiterhin beeinflussen die Hintergrundbelastung, Abspuelungen von unbefestigten Flaechen sowie weitere Aktivitaeten (z.B. intensive Bautaetigkeit) die Belastung von Nebenstrassen. Im Verlauf der untersuchten Niederschlagsereignisse war nach ca. 6 mm Niederschlag die Auswaschung der bodennahen Luftschicht beendet. Nach einem gefallenen Niederschlag von jeweils 15 mm konnte bei zwei Ereignissen die nahezu vollstaendige Abspuelung der partikulaeren Fracht festgestellt werden. Rund 80% der Schwermetallbelastung (bei Cu nur ca. 50%) des Einzugsgebietes werden durch die Emissionen des Strassenverkehrs verursacht. Kupferdaecher emittieren rund 40% der Cu-Fracht. Die Schwermetallfracht der Dachabfluessen ist ueberwiegend in der fluessigen Phase enthalten (ausser Pb, Fe), dagegen ist die Fracht der Strassenabfluesse zu ueber 90% partikulaer gebunden. Ein Rueckhalt dieser Schwermetallfracht koennte somit ueber den AFS-Rueckhalt geschehen. Entsprechend der durchgefuehrten Berechnung auf Grundlage bekannter Mittelwerte entfernt die Stadtreinigung Dresden gegenwaertig etwa die Haelfte der Fe- und Ni-Gesamtfracht sowie rund 40% der Cu-, Pb- und Zn-Gesamtfracht des Einzugsgebietes. (orig./AJ)

  6. Towards understanding the biological function of hopanoids (Invited)

    Science.gov (United States)

    Doughty, D. M.; Hunter, R.; Summons, R. E.; Newman, D. K.

    2010-12-01

    Rhodopseudomonas palustris TIE-1 expresses bacterial hopanoid lipids that are structurally similar and evolutionarily related to eukaryotic sterols. The genome of R. palustris TIE-1 contains two copies of the hpnN gene (hpnN1 and hpnN2) that are orthologs of genes encoding eukaryotic sterol and lipid transporters. Hopanoid localization to the outer membrane was found to be dependent upon hpnN1. Since the cell cycle of R. palustris TIE-1 is obligately bimodal with each cell division resulting in the generation of one mother and one swarmer cell, evidence was obtained that hopanoids where specifically localized to the outer membrane of mother cells. The sequestration of hopanoids to the mother cells was also disrupted by the deletion of the hpnN1 gene. Mutants lacking the hopanoid transporters were able to grow normally at 30 °C but showed decreased growth at 38 °C. The hopanoid transporter mutant formed cellular filaments when grown at elevated temperature. Because sedimentary steranes and hopanes comprise some of the earliest evidence for the emergence of distinct bacteria and eukaryotic phyla, a better appreciation of the function of hopanoids will improve our ability to interpret the evolution of life on Earth.

  7. AFM imaging of bacteria in liquid media immobilized on gelatin coated mica surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Doktycz, M.J.; Sullivan, C.J.; Hoyt, P.R.; Pelletier, D.A.; Wu, S.; Allison, D.P

    2003-10-15

    Immobilization of particulates, especially biomolecules and cells, onto surfaces is critical for imaging with the atomic force microscope (AFM). In this paper, gelatin coated mica surfaces are shown to be suitable for immobilizing and imaging both gram positive, Staphylococcus aureus, and gram negative, Escherichia coli, bacteria in both air and liquid environments. Gelatin coated surfaces are shown to be superior to poly-L-lysine coated surfaces that are commonly used for the immobilization of cells. This cell immobilization technique is being developed primarily for live cell imaging of Rhodopseudomonas palustris. The genome of R. palustris has been sequenced and the organism is the target of intensive studies aimed at understanding genome function. Images of R. palustris grown both aerobically and anaerobically in liquid media are presented. Images in liquid media show the bacteria is rod shaped and smooth while images in air show marked irregularity and folding of the surface. Significant differences in the vertical dimension are also apparent with the height of the bacteria in liquid being substantially greater than images taken in air. In air immobilized bacterial flagella are clearly seen while in liquid this structure is not visible. Additionally, significant morphological differences are observed that depend on the method of bacterial growth.

  8. Enhancement of photoheterotrophic biohydrogen production at elevated temperatures by the expression of a thermophilic clostridial hydrogenase.

    Science.gov (United States)

    Lo, Shou-Chen; Shih, Shau-Hua; Chang, Jui-Jen; Wang, Chun-Ying; Huang, Chieh-Chen

    2012-08-01

    The working temperature of a photobioreactor under sunlight can be elevated above the optimal growth temperature of a microorganism. To improve the biohydrogen productivity of photosynthetic bacteria at higher temperatures, a [FeFe]-hydrogenase gene from the thermophile Clostridium thermocellum was expressed in the mesophile Rhodopseudomonas palustris CGA009 (strain CGA-CThydA) using a log-phase expression promoter P( pckA ) to drive the expression of heterogeneous hydrogenase gene. In contrast, a mesophilic Clostridium acetobutylicum [FeFe]-hydrogenase gene was also constructed and expressed in R. palustris (strain CGA-CAhydA). Both transgenic strains were tested for cell growth, in vivo hydrogen production rate, and in vitro hydrogenase activity at elevated temperatures. Although both CGA-CThydA and CGA-CAhydA strains demonstrated enhanced growth over the vector control at temperatures above 38 °C, CGA-CThydA produced more hydrogen than the other strains. The in vitro hydrogenase activity assay, measured at 40 °C, confirmed that the activity of the CGA-CThydA hydrogenase was higher than the CGA-CAhydA hydrogenase. These results showed that the expression of a thermophilic [FeFe]-hydrogenase in R. palustris increased the growth rate and biohydrogen production at elevated temperatures. This transgenic strategy can be applied to a broad range of purple photosynthetic bacteria used to produce biohydrogen under sunlight.

  9. Contactless detection of the structure and selected parameters of a two-phase-flow by pattern recognition based ultrasonic techniques. Final report; Beruehrungslose Detektion der Struktur und Messung ausgewaehlter Parameter von Zweiphasenstroemungen durch Mustererkennungsverfahren unter Verwendung von Ultraschall als Messsonde. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Prasser, H.M.; Schuetz, P.; Kossok, N.

    1997-11-01

    , wenn die thermodynamischen und geometrischen Randbedingungen nahezu konstant bleiben. Durch die Erweiterung der Trainingsmatrizen um einen, die thermodynamischen Eigenschaften determinierenden Parameter (z.B. Temperatur, Druck) kann das Klassifizierungsverfahren auch auf Faelle mit veraenderlichen Stoffwerten angewandt werden. Das Mustererkennungsverfahren und die Ultraschalltechnik wurden experimentell optimiert und erprobt. Die Ultraschallsignale wurden ausserdem mit Hilfe der Methode des unueberwachten Lernens klassifiziert. Die gefundenen Gruppen von Signalmustern weisen eine deutliche Aehnlichkeit zu bekannten Stroemungskarten auf. Es wird gezeigt, dass das Verfahren des unueberwachten Lernens sich fuer die objektive Aufstellung von Stroemungskarten eignet. In einem speziellen Kapitel werden die Ergebnisse von Tests zur Fuellstandsmessung in einem Segment der Hauptumwaelzleitung von Druckwasserreaktoren vorgestellt. (orig.)

  10. MR-Imaging optimisation of the articular hip cartilage by using a T{sub 1}-weighted 3-dimensional gradient-echo sequence and the application of a hip joint traction; Magnetresonanztomographische Optimierung der Hueftknorpeldarstellung durch die Wahl einer T{sub 1}-Volumen-Gradienten-Echo-Sequenz und die Anwendung einer Hueftgelenkstraktion

    Energy Technology Data Exchange (ETDEWEB)

    Rosenberg, R. [Heidelberg Univ. (Germany). Orthopaedische Klinik und Poliklinik; Bernd, L. [Heidelberg Univ. (Germany). Orthopaedische Klinik und Poliklinik; Wrazidlo, W. [ATOS-Praxisklinik, Heidelberg (Germany). Radiologische Gemeinschaftspraxis Drs. Lederer, Schneider und Wrazidlo; Lederer, W. [ATOS-Praxisklinik, Heidelberg (Germany). Radiologische Gemeinschaftspraxis Drs. Lederer, Schneider und Wrazidlo; Schneider, S. [ATOS-Praxisklinik, Heidelberg (Germany). Radiologische Gemeinschaftspraxis Drs. Lederer, Schneider und Wrazidlo

    1995-10-01

    Images of three animal cadaver hips, 8 dissected patient femoral heads and 18 hip joints of human corpses, all either with arthrosis stage I-III or artificial cartilage defects, were compared with their corresponding anatomic sections. Additional histomorphologic examinations of the arthrotic cartilages were conducted, and MR-Imaging of 20 healthy and 21 arthrotic patient hips was performed using a specific traction method. Using a T{sub 1}-weighted 3-dimensional gradient-echo sequence and a traction of the hip joint, it was possible due to the low-signal imaging of the joint space to separate in vivo the high-signal femoral head cartilage from the high-signal acetabular cartilage. In horizontal position of the phase-encoding parameter, minimisation of the chemical-shift artifact, mainly in the ventro-lateral areas, was accomplished. MRI measurements of the articular cartilage widths showed significant correlations (p < 0.001) with the corresponding anatomic sections. At the same time the T{sub 1} 3-dimensional gradient-echo sequence of the lateral femoral head with r = 0.94 showed the lowest deviations of the measurements. It was possible with MR-Imaging to distinguish four cartilage qualities. (orig./MG) [Deutsch] Im experimentellen Teil der Studie wurden den MRT-Bildern von drei Kadavertierhueften, 8 resezierten Patientenhueftkoepfen und 18 Leichenhueftgelenken, an denen entweder artifizielle Knorpeldefekte gesetzt wurden oder die ein Koxarthrose-Stadium I-III aufwiesen, die korrespondierenden makroskopischen Kryomikrotomschnitte zugeordnet. Bei den Koxarthrosen erfolgten zusaetzliche histomorphologische Knorpeluntersuchungen. Im klinischen Teil der Studie wurden 20 gesunde und 21 arthrotische Probandenhueftgelenke mit einem speziellen Traktionsverfahren untersucht. Unter Anwendung einer T{sub 1}-Volumen-Gradienten-Echo-Sequenz und einer Traktion am zu untersuchenden Hueftgelenk konnte in vivo durch die signalarme Darstellung des Gelenkspaltes der

  11. CNS metabolism in high-risk drug abuse, German version. Insights gained from {sup 1}H- and {sup 31}P MRS and PET; ZNS-Stoffwechsel bei Missbrauch von Hochrisikodrogen. Erkenntnisse durch {sup 1}H- und {sup 31}P-MRS sowie PET

    Energy Technology Data Exchange (ETDEWEB)

    Bodea, S.V. [Klinik fuer Diagnostische und Interventionelle Neuroradiologie, Universitaetsklinikum des Saarlandes, Homburg/Saar (Germany)

    2017-06-15

    Methamphetamin-, Kokain- und Heroinkonsumenten bis Maerz 2017 wurde ueber PubMed-Recherchen erforscht. Suchbegriffe wie ''methamphetamine'', ''cocaine'', ''heroin'', ''MR spectroscopy'', ''PET'' wurden benutzt. MRT- und PET-Verfahren sind unerlaessliche Werkzeuge bei der Erforschung der zerebralen Reaktionen auf illegale Drogen. Zukuenftige Durchbrueche werden voraussichtlich durch die Untersuchung neuer Neurotransmittersysteme in der PET sowie die Abbildung von Phosphor- und Kohlenstoffmetaboliten in der MRT erfolgen. (orig.)

  12. Die Porsche-Übernahme durch VW verursacht ein Wettbewerbsproblem

    OpenAIRE

    Dudenhöffer, Ferdinand

    2009-01-01

    Der VW-Konzern hat Porsche übernommen, ohne dass Besorgnisse über wettbewerbspolitische Fragen aufkamen. Denn auf den ersten Blick hat sich die Marktmacht des gesamten Unternehmens nicht wesentlich vergrößert. Dies gilt allerdings nicht für den Premium-Markt wie Ferdinand Dudenhöffer findet.

  13. Imageverbesserung durch mediumgerechte Umsetzung der Corporate Identity im Internet

    OpenAIRE

    Gans, Thilo

    2004-01-01

    Die beiden Themenschwerpunkte werden zunächst in Teil A separat voneinander behandelt: ·Corporate Identity und Corporate Image in Kapitel I, ·E-.Business, einschließlich Internet als spezielles Medium in Kapitel II. Neben einer allgemeinen Grundlegung wollen wir insbesondere verdeutlichen, warum Corporate Identity (Eigenbild) und Corporate Image (Fremdbild) keineswegs übereinstimmen müssen, und welche negativen Folgen eine Abweichung nach sich zieht. Abschließend werden spezielle Ziele der ...

  14. Vermittlung von "Visual Literacy" durch Computeranimation im Kunstunterricht

    OpenAIRE

    Ko Hoang, Youn-Ju

    2010-01-01

    HTML> Untitled Document A b s t r a c t The aim of the present study is to give a theoretical and practical basis for teaching "Visual Literacy" in the framework of media education with emphasis on computer animation. Because of the technical possibility of reproduction and diffusion of pictures, the visual media have gained a stronger influence on our everyday life. The pictures diffused by the media do not directly reflect external reality, they r...

  15. Israelische Public Diplomacy und ihre Wahrnehmung durch deutsche Journalisten

    OpenAIRE

    Florian Fleischer; Katharina Füser; Johanna Isermeyer

    2014-01-01

    Public Diplomacy stellt insbesondere für Staaten in internationalen Konflikten wie Israel ein wichtiges Kommunikationsinstrument dar, um ausländische Bevölkerungen zu erreichen. Der Artikel zeigt die Ergebnisse einer Studie, in der untersucht wurde, wie die israelische Public Diplomacy von deutschen Journalisten aufgenommen und verarbeitet wird. Journalisten kommt dabei eine Schlüsselrolle als Mittler zwischen dem israelischen Staat und der deutschen Bevölkerung zu. In Anlehnun...

  16. Energy - achieving an optimum through information. Energie - optimal durch Information

    Energy Technology Data Exchange (ETDEWEB)

    Gitt, W.

    1986-01-01

    What have computer programs in common with everyday human behaviour. Or the birds' passage, or photosynthesis, or the chemical reactions in a cell. They all primarily are information-controlled processes. The book under review deals with 'information' and 'energy', two main concepts in today's technological world. 'Energy' during the last few years has become a significant criterion with regard to technological progress. 'Information' is not only a main term in informatics terminology, but also a central concept for example in biology, linguistics, and communication science. The author shows that every 'information' is the result of an intellectual and purposeful process. The concept of information is taken as the red thread leading the author's journey through manifold strata of modern life, asking questions, finding answers, discussing problems. The wide spectrum of aspects discussed, including for instance a new approach to the Bible, and the remarkable examples presented by the author, make this book a treasure of knowledge, and of faith.

  17. Saving energy through improved combustion; Energiesparen durch bessere Verbrennung

    Energy Technology Data Exchange (ETDEWEB)

    Stadelmann, M.

    2010-07-01

    This short article discusses how controlling oxygen supply can reduce residual oxygen in the flue gases of the burners of heating systems. The author discusses how the necessary amount of excess oxygen fed into the burner's flame can be controlled and thus minimised. In this way, the efficiency of the heating system can be improved and, as a result, energy can be saved. Also, the various factors influencing the amount of excess oxygen to be provided are discussed and typical figures for various types of burners, e.g. wood, oil, natural gas, are quoted. The control of the speed of the ventilators in the burners is also looked at. Savings in CO{sub 2} emissions and the pay-back for the oxygen control system are also discussed.

  18. Ausbreitung terrestrischer Wirbelloser durch Fließgewässer

    OpenAIRE

    2004-01-01

    Fragmentation und Isolation werden als wichtigste Gründe für den Rückgang der Biodiversität in der heutigen Kulturlandschaft angesehen. Allerdings sind generelle Schlussfolgerungen zur Fragmentation, dem Individuenaustausch zwischen Populationen sowie zur Aussterbewahrscheinlichkeit zu überprüfen, wenn sie ausschließlich Daten zur aktiven Ausbreitung bestimmter Taxa berücksichtigen. Ausbreitungsdistanzen bei passiver Verf...

  19. Degradation of phenols by microorganisms. Abbau von Phenolen durch Mikroorganismen

    Energy Technology Data Exchange (ETDEWEB)

    Klapp, K.U.; Wachtendonk, D. von (Lebensmittel- und chemisches Untersuchungsamt Aachen (Germany))

    1994-11-01

    Aerobic degradation of phenols is via oxidations, dealkylation and hydrolysis. These reactions are catalysed by enzymes. Upon completion of the reactions and elimation of eventual nitro, amino or sulfate acid residues, protocatechuate, catechol and gentisic acid remain as main intermediate products. There then follows ring splitting, which may take the form of a meta or para splitting. The end products are acetaldehyde, pyruvate, 3-oxo-adipate and fumarate, which are transferred into the citrate cycle. Protocatechuate is hard to analyse because of its reactivity. Searching for a phenol-metabolizing micro-organisms, Pseudomonas putida was insolated following a short adaptation time. Exotoxicity for Pseudomonads is at 350 mg/l phenol, but under optimized conditions it may be higher. (orig./EF)

  20. Multiple microembolizations by elemental mercury. Multiple Mikroembolisationen durch elementares Quecksilber

    Energy Technology Data Exchange (ETDEWEB)

    Wedekind, G. (Radiologische Abt., Krankenhaus Porz am Rhein (Germany)); Beyer, D. (Radiologische Abt., Krankenhaus Porz am Rhein (Germany))

    1994-08-01

    Elemental mercury is usually found in the vascular system following intravenous injection. It is distributed in the body in two different ways, which are described in detail. We report the case of a 39-year-old male, who injected elemental mercury in his cubital vein in a suicide attept. This fact was detected accidentally during clinical treatment. Microemboli of metallic mercury were found in the lungs, liver, spleen, kidneys, central nervous system and other organs. A high serum concentration of dissolved and oxidized mercury was detected. No signs of chronic mercury intoxication in the form an organic functional disturbance were obvious. (orig.)

  1. Erfahrbares Programmieren durch den zielgerichten Einsatz von Flugdrohnen im Informatikunterricht

    Directory of Open Access Journals (Sweden)

    Raphael Zender

    2015-11-01

    Full Text Available Today, teaching on fundamental programming skills is one of the essential IT-related topics in schools. This trend is brought forward by simple development environments like Scratch. Those abstract from detailed programming concepts in terms of graphical building blocks. Exotic runtime platforms like robots further increase the attractiveness. The solution “ScratchDrone” provides quadrocopters as an innovative new platform to run Scratch programs. The modular system architecture of ScratchDrone allows children to program on multiple layers of abstraction depending on their individual learning progress. The combination of a multi-layer didactical model, the movement in 3D space, as well as the natural human fascination for flying results in a high learning motivation for young programmers.

  2. Die Leben Einsteins eine Reise durch die Geschichte der Physik

    CERN Document Server

    Fiami

    2005-01-01

    Jeder kennt die Namen Einstein, Newton oder Galilei. aber was weiss man über sie? Hier ein Porträt Einsteins anhand von sechs Meilensteinen aus der Geschichte der Physik. Einstein tritt auf als Protagonist in verschiedenen Epochen und bei verschiedenen Entdeckungen, die die Welt verändert haben.

  3. Cost reduction through combined solutions?; Kostensenkung durch Kombinationsloesungen?

    Energy Technology Data Exchange (ETDEWEB)

    Bilitewski, B. [Technische Univ. Dresden (Germany). Inst. fuer Abfallwirtschaft und Altlasten

    1998-09-01

    The implementation of material-specific residue treatment processes requires reconstruction measures both in the case of mechanical-biological and in that of thermal treatment plants. Such measures involves great uncertainties and wide variation in cost prediction. The present paper deals with various factors that influence the costs of residual waste treatment. [Deutsch] Zur Umsetzung der stoffspezifischen Restabfallbehandlung sind Anpassungen sowohl der derzeit betriebenen mechanisch-biologischen als auch der thermischen Anlagen erforderlich, was zu Unsicherheiten und grossen Schwankungsbreiten bei den Kostenprognosen fuehrt. Der Bericht beleuchtet verschiedene Einflussgroessen auf die Kosten der Behandlung des Restabfalls. (orig./SR)

  4. Ionizing radiation induced cataract; Katarakt-Induktion durch ionisierende Strahlung

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, W.U. [Universitaetsklinikum Essen (Germany). Inst. fuer Medizinische Strahlenbiologie

    2013-07-01

    Until recently it was believed that the cataract (opacity of the eye lens) is a deterministic effect with a dose threshold of several Gray in dependence on the exposure conditions. Studies in Hiroshima and Nagasaki, in the vicinity of Chernobyl, of American radiologic technologists, astronauts, and patients after having received several computer tomographies of the head region, however, have shown that this assumption is not correct. It had been overlooked in the past that with decreasing dose the latency period is increasing. Therefore, the originally available studies were terminated too early. The more recent studies show that, in the case of a threshold existing at all, it is definitely below 0.8 Gy independently of an acute or a chronic exposure. All studies, however, include 0 Gy in the confidence interval, so that the absence of a dose threshold cannot be excluded. The German Commission on Radiological Protection (Strahlenschutzkommission, SSK) suggested therefore among others: targeted recording of the lens dose during activities which are known to be associated with possible significant lens exposure, examination of the lens should be included as appropriate in the medical monitoring of people occupationally exposed to radiation, if there is potentially high lens exposure, adoption of research strategies to develop a basic understanding of the mechanisms underlying radiation induced cataracts. The International Commission on Radiological Protection (ICRP) actually assumes a threshold dose of 0.5 Gy and, based on this assumption, has recommended in 2011 to reduce the dose limit for the eye lens from 150 mSv in a year to 20 mSv in a year for people occupationally exposed to ionising radiation. (orig.)

  5. A history of solar architecture; Zeitreise durch die Solararchitektur

    Energy Technology Data Exchange (ETDEWEB)

    Hastings, R. [AEU GmbH, Wallisellen (Switzerland)

    2005-07-01

    This journey from the beginning of the 20th century to the present examines the developments in building with the sun with a focus on housing. It begins in the USA and then follows parallel paths in Europe, ending with a prognosis for the future. The way is marked by milestones in the form of technological breakthroughs or changes in politics or economics which dramatically affected the advance in ''solar architecture''. Two different courses are tracked: the engineering approach using active solar and technical systems and the architecture approach where the building is the collector, storage and distributor of solar heat. Both ways influence the aesthetics of a house. How energy concepts have been integrated into architecture has led to fascinating design variations, from the Californian hippy solar houses in the 1970's to modern industrial design housing. Along the journey some ways have proven to be dead-ends, some allowed accelerated progress. It is interesting to learn from the journey in order to make wise choices as we continue our way (orig.)

  6. Optimierung mikrobieller Produktionsstämme durch quantitative Proteomanalysen

    OpenAIRE

    Wolff, Daniel

    2011-01-01

    Das Ziel der vorliegenden Arbeit war die quantitative Analyse der Organismen Escherichia coli und Pichia ciferrii mittels gerichteter und ungerichteter Proteomik. Auf diese Weise sollten für die Bioindustrie relevante Stoffwechselwege gezielt untersucht, eventuelle bottle necks identifiziert sowie Merkmale des Zentralmetabolismus aufgeklärt werden. In Bezug auf den E. Coli-Stamm, einem heterologen Produzenten des Polyphenols Resveratrol, konnten neun Kandidaten eines putativen ...

  7. Innovation durch Partnering im deutschen Schlüsselfertigbau

    OpenAIRE

    Schmolke, Daniel

    2008-01-01

    In der angloamerikanischen Baubranche werden bereits seit Jahren innovative Managementkonzepte zur Steuerung partnerschaftlicher Kooperationen nach dem Leitgedanken des Partnering zwischen Haupt- und Nachunternehmern im Schlüsselfertigbau erfolgreich umgesetzt. Hierzulande bleibt der Einsatz derartiger Partnering-Modelle bislang jedoch weitgehend unbeachtet. Das Ziel der Arbeit besteht daher darin, wesentliche Elemente und Erfahrungen des angloamerikanischen Partnering-Konzeptes im Hinb...

  8. Integration durch Integrationskurse? Eine Verlaufsstudie bei iranischen Migrantinnen und Migranten

    OpenAIRE

    Yazdani, Keyghobad

    2014-01-01

    Die Dissertation beschäftigt sich aus qualitativer Perspektive mit der subjektiven Sichtweise der iranischen Migranten (im Lande Bremen) zum Ertrag der Integrationskurse zu ihrer sprachlichen und sozialen Integration in Deutschland. Die Schwerpunkte der Studie waren: Spracherwerb und sprachliche Integration , Akkulturation und soziokulturelle Integration und Selbstkonzept und persönliche Einstellungen und Befindlichkeiten . Das Endergebnis der Studie lautet: Integrationskurse in diesem Umfang...

  9. Neurologische Diagnostik unterstützt durch molekulargenetische Untersuchungen

    Directory of Open Access Journals (Sweden)

    Parigger S

    2002-01-01

    Full Text Available Patienten mit lange bestehenden neurologischen Defiziten, deren Diagnosen vor Jahren oder Jahrzehnten gestellt wurden, verlocken nicht sehr zur neuerlichen Durchuntersuchung. Die Diagnose "Multiple Sklerose", in den 70er Jahren gestellt, ohne Aussicht auf Therapie, hielt Herrn F. M. in den folgenden Jahren von Neurologischen Abteilungen fern. Im Oktober 2000 wird an der kardiologischen Abteilung des Hauses ein 66jähriger Mann wegen Angina pectoris-Beschwerden und klinischen Zeichen von Linksherzdekompensation aufgenommen. Zur neurologischen Begutachtung kommt ein Mann in reduziertem Ernährungszustand (EZ, der rollstuhlpflichtig ist. Er hat angeblich Multiple Sklerose (MS; eine rezente Kraftminderung und Ungeschicklichkeit der linken Oberextremität (OE wurden festgestellt.

  10. Erschließung externer Ressourcen durch Unternehmensnetzwerke

    Science.gov (United States)

    Bergmann, Lars; Lacker, Thomas

    Jedes Unternehmensnetzwerk basiert auf einem sozialen Netzwerk. Einer häufig zitierten Definition zufolge ist dies: " (…) a specific set of linkages among a defined set of actors, with the additional property that the characteristics of these linkages as a whole may be used to interpret the social behaviour of the actors involved.“ (Mitchell 1969)

  11. Schnelle Rasterkraftmikroskopie durch moderne Regelungstechnik und mechatronische Systemintegration

    NARCIS (Netherlands)

    Steininger, J.; Kuiper, S.; Schitter, G.; Ito, S.

    2012-01-01

    In atomic force microscopy (AFM) high-performance and high precision control of the scanning-system is crucial. At high imaging speeds the dynamic behaviour of the scanner may cause imaging artefacts limiting the maximum imaging rate. This contribution discusses recent improvements for faster imagin

  12. Landeskunde durch Weinkunde: A Taste of German Culture.

    Science.gov (United States)

    Peacock, Dennis E.

    1978-01-01

    Describes a cultural unit centered on German wines. After a series of preparatory lessons showing how the topic touches on geography, history, and social customs, the unit culminated in a wine tasting. (EJS)

  13. Vollzug durch das Bild : Vertrag und Eid als Sakrament

    OpenAIRE

    Schlie, Heike

    2012-01-01

    Jede Kultur operiert mit Zeichen, und jede Zeichenoperation birgt in sich ein Konzept der Beziehung zwischen Zeichen und Bezeichnetem. Schriftlich gefasste Theorien zu dieser Beziehung entstehen in Mittelalter und Früher Neuzeit vor allem da, wo die Zeichenoperation von hoher Relevanz und dabei in ihrer genauen Bedeutung strittig ist. In der christlichen Kultur der Vormoderne hat die theologische Notwendigkeit einer Exegese der Konsekrationsformel hoc est corpus meum zu einer Arbeit am Zeiche...

  14. Gesellschaftliche Teilhabe Älterer durch Alltagsmobilität

    Directory of Open Access Journals (Sweden)

    Helga Pelizäus-Hoffmeister

    2013-11-01

    Full Text Available Alltagsmobilität – die Mobilität in der näheren Wohnumgebung – gilt in der aktuellen Mobilitätsforschung als ein wesentliches Fundament gesellschaftlicher Teilhabe. Zahlreiche Studien verweisen darauf, dass sie mit zunehmendem Alter sinkt, was dementsprechend mit einem Verlust an gesellschaftlicher Integration und sinkender Lebensqualität assoziiert wird. Dennoch existieren bislang keine Erkenntnisse darüber, wie dieser Prozess auf der Subjektebene konkret gestaltet wird, wie Veränderungen vor dem Hintergrund welcher (räumlichen Wahrnehmungen und Überzeugungen von den Individuen initiiert werden. Hier setzt die dem Beitrag zugrunde liegende Untersuchung an: Das Ziel ist es, auf der Basis einer qualitativ orientierten Untersuchung herauszuarbeiten, wie Veränderungen der Mobilität im Kontext der subjektiven Konstruktionsleistungen und Handlungen der Älteren in einer gegenstandsorientierten Theorie gefasst und erklärt werden können. In einem zweiten Schritt werden prospektiv Thesen darüber entwickelt, in welcher Weise der Einsatz eines technischen Hilfsmittels – eines "Mobilitätsassistenten", der im Rahmen eines vom BMBF (Bundesministerium für Bildung und Forschung geförderten Projekts entwickelt wird – auf die Wahrnehmungen und Überzeugungen der Älteren Einfluss nehmen und darauf aufbauend deren Mobilität fördern könnte. URN: http://nbn-resolving.de/urn:nbn:de:0114-fqs1401113

  15. Energy transparency by business intelligence; Energietransparenz durch Business Intelligence

    Energy Technology Data Exchange (ETDEWEB)

    Flieder, Karl [Academia Nova, Schwechat (Austria). Business Process Management and Engineering

    2012-10-15

    The efficiency of energy is one of several key performance parameters, when it comes to the questions how innovative and economical a plant operates compared to its competitors. However, in order to evaluate energy saving potentials in the industry, we need transparency first: A clear picture of the energy consumption based on products and processes. This transparency can be accomplished through business intelligence (BI). This term encompasses analytical processes and tools that can help to transform enterprise data and information into applied knowledge. In this article, we focus on transparency of the energy consumption through a targeted analysis by making use of business intelligence.

  16. Sustainable development through continuous optimization; Nachhaltige Entwicklung durch kontinuierliche Optimierung

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2011-07-01

    Lafarge operates one of Germany's most modern cement plants in Woessingen (Fig. 1). With the changeover from the semi-dry (Lepol) process to the dry process, which was completed in 2009 after only 17 months, the products were improved, the emissions were significantly reduced and the fuel mix was optimized. ZKG INTERNATIONAL has talked to the plant manager, Lutz Weber, about his experience with operating the new plant (see webcode ZKG0M6RR). Continuous optimization and modernization is traditional at Woessingen. Over the years this has enabled it to maintain its competitiveness and improve it still further. An open door day was held in September 2010 on the 60{sup th} anniversary of the cement plant at Woessingen to which politicians, neighbours and customers were invited to see the current state of the plant for themselves. Thanks to the new production line with a 5-stage cyclone preheater with calciner and combustion chamber from A TEC (Fig. 2) the way is open for a successful future for the Lafarge plant at Woessingen. (orig.)

  17. Allergien durch Stiche und Bisse von Insekten und Spinnentieren

    DEFF Research Database (Denmark)

    Blank, S; Schiener, M; Ollert, M.

    2016-01-01

    Allergic reactions due to stings of Hymenoptera species, such as honeybees or yellow jackets, are well known as severe allergies with potentially fatal outcome. Much less common is that also bites of blood-sucking insects (mosquitos, horseflies, flies, bugs, lice and fleas) and arachnids (ticks...

  18. Rechtsmissbrauch durch „AGG-Hopping“ / Steffen Krieger

    Index Scriptorium Estoniae

    Krieger, Steffen

    2016-01-01

    Euroopa Kohtu otsusest, mis puudutab töökohale kandideerija staatust eesmärgiga esitada kahju hüvitamise nõudeid kui teda ei valita tööle ja sellise teguviisi käsitlemist õiguse kuritarvitamisena. Vt. ka lk. 699-700

  19. Defect size dependent contrast reduction and additional blurring from scattered radiation reduce the quality of radiological images. A comparison of films and digital detectors; Fehlergroessenabhaengige Kontrastreduktion und zusaetzliche Unschaerfe durch Streustrahlung reduzieren die Bildqualitaet radiologischer Abbildungen. Film und digitale Detektoren im Vergleich

    Energy Technology Data Exchange (ETDEWEB)

    Ewert, Uwe; Beckmann, Joerg; Bellon, Carsten; Jaenisch, Gerd-Ruediger; Zscherpel, Uwe; Jechow, Mirko [Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin (Germany); Grosser, Anja [Technische Fachhochschule Wildau (Germany)

    2008-07-01

    wurde eine zusaetzliche Kontrastreduktion, her-vorgerufen durch Streustrahlung, gefunden, wenn der Detektor sich nahe am Objekt befindet (Kontakttechnik). Der Effekt haengt von der Fehlergroesse, dem Abstand zwi-schen Detektor und Objekt und der Zwischenfilterung ab.

  20. Reduzierbarkeit der Abbrecherquote im Humanmedizinstudium durch das Hamburger Auswahlverfahren für Medizinische Studiengänge - Naturwissenschaftsteil (HAM-Nat [Possible reduction of the medical school dropout number by the Hamburg Assessment test for Medicine - part Natural sciences (HAM-Nat

    Directory of Open Access Journals (Sweden)

    Harendza, Sigrid

    2008-05-01

    eines zukünftigen „guten Arztes“ zu entwickeln und zu validieren. Daher beschränkt sich das Auswahlverfahren in Hamburg auf das pragmatische Ziel die Studienabbrecherzahl zu reduzieren, damit möglichst viele Studienanfänger durch gute Lehre zu „guten Ärzten“ ausgebildet werden. Methodik: Zu diesem Zweck wurde der HAM-Nat (Hamburger Auswahlverfahren für Medizinische Studiengänge - Naturwissenschaftsteil mit 52 Multiple-choice Fragen auf Schulniveau über naturwissenschaftliche Kenntnisse und ihre Anwendung entwickelt, zunächst an 6 Schulen in 3 Bundesländern erprobt und dann mit 333 Studienanfängern im Oktober 2006 durchgeführt. Das Testergebnis und die Abiturnote wurden mit dem Studienerfolg in den ersten beiden Semestern korreliert. Als Studienerfolgsparameter wurde die Anzahl der bestandenen Leistungsnachweise verwendet. Ergebnisse: Das HAM-Nat-Ergebnis korreliert nach dem 2. Semester höher mit dem Studienerfolg als die Abiturnote, von der es relativ unabhängig ist. Insbesonders zeigt die Simulation eines Auswahlverfahrens unter Berücksichtigung von HAM-Nat-Ergebnis und Abiturnote eine deutliche Reduktion der Zahl der Studienabbrecher im Vergleich zur jetzigen Auswahl nur nach Abiturnote. Weitere abgefragte mögliche Studienauswahlparameter wie belegte naturwissenschaftliche Kurse oder eine abgeschlossene Berufsausbildung korrelieren nicht mit besserem Studienerfolg. Schlussfolgerung: Der HAM-Nat ist ein wertvolles zusätzliches Auswahlverfahren für Studienbewerber in der Medizin. Aufgrund dieser Ergebnisse hat die Medizinische Fakultät Hamburg beschlossen, den HAM-Nat für die Studienbewerberauswahl zum Wintersemester 2008/2009 einzuführen.

  1. Calvin cycle mutants of photoheterotrophic purple nonsulfur bacteria fail to grow due to an electron imbalance rather than toxic metabolite accumulation.

    Science.gov (United States)

    Gordon, Gina C; McKinlay, James B

    2014-03-01

    Purple nonsulfur bacteria grow photoheterotrophically by using light for energy and organic compounds for carbon and electrons. Disrupting the activity of the CO2-fixing Calvin cycle enzyme, ribulose 1,5-bisphosphate carboxylase (RubisCO), prevents photoheterotrophic growth unless an electron acceptor is provided or if cells can dispose of electrons as H2. Such observations led to the long-standing model wherein the Calvin cycle is necessary during photoheterotrophic growth to maintain a pool of oxidized electron carriers. This model was recently challenged with an alternative model wherein disrupting RubisCO activity prevents photoheterotrophic growth due to the accumulation of toxic ribulose-1,5-bisphosphate (RuBP) (D. Wang, Y. Zhang, E. L. Pohlmann, J. Li, and G. P. Roberts, J. Bacteriol. 193:3293-3303, 2011, http://dx.doi.org/10.1128/JB.00265-11). Here, we confirm that RuBP accumulation can impede the growth of Rhodospirillum rubrum (Rs. rubrum) and Rhodopseudomonas palustris (Rp. palustris) RubisCO-deficient (ΔRubisCO) mutants under conditions where electron carrier oxidation is coupled to H2 production. However, we also demonstrate that Rs. rubrum and Rp. palustris Calvin cycle phosphoribulokinase mutants that cannot produce RuBP cannot grow photoheterotrophically on succinate unless an electron acceptor is provided or H2 production is permitted. Thus, the Calvin cycle is still needed to oxidize electron carriers even in the absence of toxic RuBP. Surprisingly, Calvin cycle mutants of Rs. rubrum, but not of Rp. palustris, grew photoheterotrophically on malate without electron acceptors or H2 production. The mechanism by which Rs. rubrum grows under these conditions remains to be elucidated.

  2. Exhaustive Database Searching for Amino Acid Mutations in Proteomes

    Energy Technology Data Exchange (ETDEWEB)

    Hyatt, Philip Douglas [ORNL; Pan, Chongle [ORNL

    2012-01-01

    Amino acid mutations in proteins can be found by searching tandem mass spectra acquired in shotgun proteomics experiments against protein sequences predicted from genomes. Traditionally, unconstrained searches for amino acid mutations have been accomplished by using a sequence tagging approach that combines de novo sequencing with database searching. However, this approach is limited by the performance of de novo sequencing. The Sipros algorithm v2.0 was developed to perform unconstrained database searching using high-resolution tandem mass spectra by exhaustively enumerating all single non-isobaric mutations for every residue in a protein database. The performance of Sipros for amino acid mutation identification exceeded that of an established sequence tagging algorithm, Inspect, based on benchmarking results from a Rhodopseudomonas palustris proteomics dataset. To demonstrate the viability of the algorithm for meta-proteomics, Sipros was used to identify amino acid mutations in a natural microbial community in acid mine drainage.

  3. Generation of Hydrogen from Photolysis of Organic Acids by Photosynthetic Bacteria

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Photodecomposition of ten kinds of organic acids by Rhodopseudomonas palustris for producing hydrogen has been investigated. By using acetate as hydrogen donor, dynamics of hydrogen production and cell growth has been determined; the influences of acetate concentration, temperature, light intensity and the effects of the interaction among metal ions (Fe3+, Ni2+), acetate and glutamate in aqueous solution on hydrogen production have been examined for optimizing the conditions of H2 generation. The results show that H2 production is partially correlated with cell growth; Ni2+ inhibits hydrogen production, but enhances cell growth; Fe3+ promotes hydrogen production evidently. The highest rate of H2 production is 22.1 mL L-1 h-1 under the conditions of 35 ~ 37℃, 6000 ~ 8000 lx, 30 mmolL-1 of acetate, 9 mmolL-1 of glutamate, and 50 (molL-1 of Fe3+.

  4. Redox cycling of Fe(II) and Fe(III) in magnetite by Fe-metabolizing bacteria

    Science.gov (United States)

    Byrne, James M.; Klueglein, Nicole; Pearce, Carolyn; Rosso, Kevin M.; Appel, Erwin; Kappler, Andreas

    2015-03-01

    Microorganisms are a primary control on the redox-induced cycling of iron in the environment. Despite the ability of bacteria to grow using both Fe(II) and Fe(III) bound in solid-phase iron minerals, it is currently unknown whether changing environmental conditions enable the sharing of electrons in mixed-valent iron oxides between bacteria with different metabolisms. We show through magnetic and spectroscopic measurements that the phototrophic Fe(II)-oxidizing bacterium Rhodopseudomonas palustris TIE-1 oxidizes magnetite (Fe3O4) nanoparticles using light energy. This process is reversible in co-cultures by the anaerobic Fe(III)-reducing bacterium Geobacter sulfurreducens. These results demonstrate that Fe ions bound in the highly crystalline mineral magnetite are bioavailable as electron sinks and electron sources under varying environmental conditions, effectively rendering magnetite a naturally occurring battery.

  5. A multiphase mixture model for substrate concentration distribution characteristics and photo-hydrogen production performance of the entrapped-cell photobioreactor.

    Science.gov (United States)

    Guo, Cheng-Long; Cao, Hong-Xia; Pei, Hong-Shan; Guo, Fei-Qiang; Liu, Da-Meng

    2015-04-01

    A multiphase mixture model was developed for revealing the interaction mechanism between biochemical reactions and transfer processes in the entrapped-cell photobioreactor packed with gel granules containing Rhodopseudomonas palustris CQK 01. The effects of difference operation parameters, including operation temperature, influent medium pH value and porosity of packed bed, on substrate concentration distribution characteristics and photo-hydrogen production performance were investigated. The results showed that the model predictions were in good agreement with the experimental data reported. Moreover, the operation temperature of 30 °C and the influent medium pH value of 7 were the most suitable conditions for photo-hydrogen production by biodegrading substrate. In addition, the lower porosity of packed bed was beneficial to enhance photo-hydrogen production performance owing to the improvement on the amount of substrate transferred into gel granules caused by the increased specific area for substrate transfer in the elemental volume.

  6. Arsenic removal from contaminated soil via biovolatilization by genetically engineered bacteria under laboratory conditions

    Institute of Scientific and Technical Information of China (English)

    Shuang Liu; Fan Zhang; Jian Chen; Guoxin Sun

    2011-01-01

    In Rhodopseudomonas palustris,an arsM gene,encoding bacterial and archaeal homologues of the mammalian Cyt19 As(ⅢH)S-adenosylmethionine methytransferase,was regulated by arsenicals.An expression of arsM was introduced into strains for the methylation of arsenic.When arsM was expressed in Sphingomonas desiccabilis and Bacillus idriensis,it had 10 folds increase of methyled arsenic gas compared to wild type in aqueous system.In soil system,about 2.2%-4.5% of arsenic was removed by biovolatilization during 30 days.This study demonstrated that arsenic could be removed through volatilization from the contaminated soil by bacteria which have arsM gene expressed.These results showed that it is possible to use microorganisms expressing arsM as an inexnensive,effìcient strategy for arsenic bioremediation from contaminated water and soil.

  7. The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium

    Energy Technology Data Exchange (ETDEWEB)

    Brotosudarmo, Tatas H. P. [Univ. of Glasgow, Scotland (United Kingdom). Inst. of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences; Collins, Aaron M. [Washington Univ., St. Louis, MO (United States). Depts. of Biology and Chemistry; Gall, Andrew [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France). Inst. de Biologie et Technologies de Saclay et CNRS; Roszak, Aleksander W. [Univ. of Glasgow, Scotland (United Kingdom). Dept. of Chemistry, WestChem; Gardiner, Alastair T. [Univ. of Glasgow, Scotland (United Kingdom). Inst. of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences; Blankenship, Robert E. [Washington Univ., St. Louis, MO (United States). Depts. of Biology and Chemistry; Cogdell, Richard J. [Univ. of Glasgow, Scotland (United Kingdom). Inst. of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences

    2011-11-15

    The differing composition of LH2 (peripheral light-harvesting) complexes present in Rhodopseudomonas palustris 2.1.6 have been investigated when cells are grown under progressively decreasing light intensity. Analysis of the absorption spectra reveals there must be more than two types of LH2 complexes present. Purified HL (high-light) and LL (low-light) LH2 complexes have mixed apoprotein compositions. The HL complexes contain PucABa and PucABb apoproteins. The LL complexes contain PucABa, PucABd and PucBb-only apoproteins. This mixed apoprotein composition can explain their resonance Raman spectra.

  8. The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium.

    Science.gov (United States)

    Brotosudarmo, Tatas H P; Collins, Aaron M; Gall, Andrew; Roszak, Aleksander W; Gardiner, Alastair T; Blankenship, Robert E; Cogdell, Richard J

    2011-11-15

    The differing composition of LH2 (peripheral light-harvesting) complexes present in Rhodopseudomonas palustris 2.1.6 have been investigated when cells are grown under progressively decreasing light intensity. Detailed analysis of their absorption spectra reveals that there must be more than two types of LH2 complexes present. Purified HL (high-light) and LL (low-light) LH2 complexes have mixed apoprotein compositions. The HL complexes contain PucAB(a) and PucAB(b) apoproteins. The LL complexes contain PucAB(a), PucAB(d) and PucB(b)-only apoproteins. This mixed apoprotein composition can explain their resonance Raman spectra. Crystallographic studies and molecular sieve chromatography suggest that both the HL and the LL complexes are nonameric. Furthermore, the electron-density maps do not support the existence of an additional Bchl (bacteriochlorophyll) molecule; rather the density is attributed to the N-termini of the α-polypeptide.

  9. GeO2-SiO2-chitosan-medium-coated hollow optical fiber for cell immobilization.

    Science.gov (United States)

    Zhong, Nian-Bing; Zhu, Xun; Liao, Qiang; Wang, Yong-Zhong; Chen, Rong

    2013-08-15

    A GeO(2)-SiO(2)-chitosan-medium (GSCM)-coated hollow optical fiber (HOF) is proposed. The HOF consists of three parts: the fiber core (air), cladding (SiO(2)), and coating (GSCM), which shows the highest refractive index of the three. The HOF's luminescence properties and surface morphology are investigated. Their adsorption capacity for Rhodopseudomonas palustris CQK 01 is also assayed. We discovered that when the amount of 2GeO(2)-SiO(2) sol dopant is 0.9 mass percent, the HOF exhibits the highest luminous intensity and uniform light distribution, and the adsorption capacity for the cell is 3.2 times higher than that of a normal solid optical fiber.

  10. Neural network modeling of the light profile in a novel photobioreactor.

    Science.gov (United States)

    Salazar-Peña, R; Alcaraz-González, V; González-Álvarez, V; Snell-Castro, R; Méndez-Acosta, H O

    2014-06-01

    An artificial neural network (ANN) was implemented to model the light profile pattern inside a photobioreactor (PBR) that uses a toroidal light arrangement. The PBR uses Tequila vinasses as culture medium and purple non-sulfur bacteria Rhodopseudomonas palustris as biocatalyzer. The performance of the ANN was tested for a number of conditions and compared to those obtained by using deterministic models. Both ANN and deterministic models were validated experimentally. In all cases, at low biomass concentration, model predictions yielded determination coefficients greater than 0.9. Nevertheless, ANN yielded the more accurate predictions of the light pattern, at both low and high biomass concentration, when the bioreactor radius, the depth, the rotational speed of the stirrer and the biomass concentration were incorporated in the ANN structure. In comparison, most of the deterministic models failed to correlate the empirical data at high biomass concentration. These results show the usefulness of ANNs in the modeling of the light profile pattern in photobioreactors.

  11. Energy conservation with alternative `silent` (passive) cooling systems in office buildings and energetic evaluation of cold generation for `silent cooling`. German contribution to the IEA Programme ECBS Annex 28, low energy cooling. Final report; Energieeinsparung durch den Einsatz von alternativen Systemen der `stillen` (passiven) Kuehlung in Buerogebaeuden und energetische Bewertung der Kaelteerzeugung fuer die `stille Kuehlung`. Deutsche Beteiligung an dem IEA-Programm ECBS Annex 28, low energy cooling. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Laabs, K.D.; Wolkenhauer, H. [STULZ GmbH, Hamburg (Germany); Heinrich, G.; Franzke, U.; Seifert, C. [Institut fuer Luft- und Kaeltetechnik GmbH, Dresden (Germany); Steimle, F.; Mengede, B. [Essen Univ. (Gesamthochschule) (Germany). Inst. fuer Angewandte Thermodynamik und Klimatechnik

    1997-06-01

    wissenschaftlichen Instituten ein ausgewaehltes System untersucht. Das System besteht vornehmlich aus unterschiedlichsten Formen und Anordnungen von Raumkuehlflaechen und/oder Waermetauschern, die in einem Standardbuero installiert sind. Durch Temperaturdifferenzen zwischen Raumkuehlflaechen und Raumluft stellt sich die notwendige Luftbewegung ohne mechanische Unterstuetzung ein. Hierbei wird im wesentlichen nur Wasser transportiert, welches als Energietraeger ein bedeutend geringeres Volumen als Luft benoetigt und damit zu Energieeinsparung fuehrt. Dieses System wurde in mehreren Varianten Versuchen unterzogen und bewertet mit dem Ziel, ein Auslegungswerkzeug zu erarbeiten. (orig.)

  12. Improved hydrogen production by coupled systems of hydrogenase negative photosynthetic bacteria and fermentative bacteria in reverse micelles

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Anita [Centre for Biotechnology, University of Allahabad, Allahabad 211002 (India); Misra, Krishna [Indo-Russian Center for Bioinformatics, Indian Institute of Information Technology, Allahabad 211011 (India)

    2008-11-15

    Significant improvement in biological hydrogen production is achieved by the use of coupled bacterial cells in reverse micellar systems. Two coupled systems (a) Rhodopseudomonas palustris CGA009/Citrobacter Y19, and (b) Rhodobacter sphaeroides 2.4.1/Citrobacter Y19 bacteria have been immobilized separately in aqueous pool of the reverse micelles fabricated by various surfactants (AOT, CBAC and SDS) and apolar organic solvents (benzene and isooctane). The gene for uptake hydrogenase enzyme has been manipulated further for hydrogen generation. Mutants deficient in uptake hydrogenase (Hup{sup -}) were obtained from R. palustris CGA009 and R. sphaeroides 2.4.1, and entrapped with Citrobacter Y19 in the reverse micellar systems. More than two fold increase in hydrogen production was obtained by the use of Hup{sup -} mutants instead of wild-type photosynthetic bacteria together with Citrobacter Y19. Addition of sodium dithionite, a reducing agent to AOT/H{sub 2}O/isooctane reverse micellar system with the coupled systems of wild-type photosynthetic bacteria and fermentative bacterium Y19 effected similar increase in hydrogen production rate as it is obtained by the use of mutants. CBAC/H{sub 2}O/isooctane reverse micellar system is used for the first time for hydrogen production and is as promising as AOT/H{sub 2}O/isooctane reverse micellar system. All reverse micellar systems of coupled bacterial cultures gave encouraging hydrogen production (rate as well as yield) compared to uncoupled bacterial culture. (author)

  13. Tracking energy transfer between light harvesting complex 2 and 1 in photosynthetic membranes grown under high and low illumination.

    Science.gov (United States)

    Lüer, Larry; Moulisová, Vladimíra; Henry, Sarah; Polli, Dario; Brotosudarmo, Tatas H P; Hoseinkhani, Sajjad; Brida, Daniele; Lanzani, Guglielmo; Cerullo, Giulio; Cogdell, Richard J

    2012-01-31

    Energy transfer (ET) between B850 and B875 molecules in light harvesting complexes LH2 and LH1/RC (reaction center) complexes has been investigated in membranes of Rhodopseudomonas palustris grown under high- and low-light conditions. In these bacteria, illumination intensity during growth strongly affects the type of LH2 complexes synthesized, their optical spectra, and their amount of energetic disorder. We used a specially built femtosecond spectrometer, combining tunable narrowband pump with broadband white-light probe pulses, together with an analytical method based on derivative spectroscopy for disentangling the congested transient absorption spectra of LH1 and LH2 complexes. This procedure allows real-time tracking of the forward (LH2 → LH1) and backward (LH2←LH1) ET processes and unambiguous determination of the corresponding rate constants. In low-light grown samples, we measured lower ET rates in both directions with respect to high-light ones, which is explained by reduced spectral overlap between B850 and B875 due to partial redistribution of oscillator strength into a higher energetic exciton transition. We find that the low-light adaptation in R. palustris leads to a reduced elementary backward ET rate, in accordance with the low probability of two simultaneous excitations reaching the same LH1/RC complex under weak illumination. Our study suggests that backward ET is not just an inevitable consequence of vectorial ET with small energetic offsets, but is in fact actively managed by photosynthetic bacteria.

  14. Preferential incorporation of coloured-carotenoids occurs in the LH2 complexes from non-sulphur purple bacteria under carotenoid-limiting conditions.

    Science.gov (United States)

    Gall, Andrew; Henry, Sarah; Takaichi, Shinichi; Robert, Bruno; Cogdell, Richard J

    2005-11-01

    The effect of growing Rhodopseudomonas (Rps.) acidophila and Rps. palustris in the presence of different concentrations of the carotenoid (Car) biosynthetic inhibitor diphenylamine (DPA) has been investigated. Growth with sub-maximal concentrations of DPA induces Car limitation. The exact response to DPA is species dependent. However, both Rps. acidophila and Rps. palustris respond by preferentially incorporating the limiting amount of coloured Cars into their LH2 complexes at the expense of the RC-LH1 complexes. As inhibition by DPA becomes more severe there is an increase in the percentage of Cars with reduced numbers of conjugated C=C bonds. The effect of this changed Car composition on the structure and function of the antenna complexes has been investigated using absorption, fluorescence, CD and Raman spectroscopies. The results show that although the presence of Car molecules is important for the stability of the LH2 complexes that the overall native structure can be maintained by the presence of many different Cars.

  15. Assembling and exploring the Cochliobolus miyabeanus genome of a strain pathogenic on wildrice (Zizania palustris)

    Science.gov (United States)

    The genome of a strain of C. miyabeanus was shotgun sequenced by paired-end reads with Illumina HiSeq 2000 technology. The genome was assembled with AbySS software yielding a total size of 34.96 Mb (114X), with N50 = 99.43 kb contained in the largest 105 scaffolds and a maximum scaffold length of 40...

  16. Time series analysis of forest carbon dynamics: recovery of Pinus palustris physiology following a prescribed fire

    Science.gov (United States)

    G. Starr; C. L. Staudhammer; H. W. Loescher; R. Mitchell; A. Whelan; J. K. Hiers; J. J. O’Brien

    2015-01-01

    Frequency and intensity of fire determines the structure and regulates the function of savanna ecosystems worldwide, yet our understanding of prescribed fire impacts on carbon in these systems is rudimentary. We combined eddy covariance (EC) techniques and fuel consumption plots to examine the short-term response of longleaf pine forest carbon dynamics to one...

  17. Characterizing Soil Organic Carbon Recalcitrance in Longleaf Pine (Pinus palustris Mill) Stands

    Science.gov (United States)

    Butnor, J. R.; Samuelson, L. J.; Anderson, P. H.; Gonzalez-Benecke, C. A.; Boot, C. M.; Cotrufo, M. F.; Heckman, K. A.; Jackson, J. A.; Johnsen, K. H.; Stokes, T.; Swanston, C.

    2015-12-01

    Historically, longleaf pine (LLP) stands in the southeastern US experienced frequent fires. Today managed LLP stands are burned at 2-5 year intervals to reduce fuels and hardwood competition and manage for biodiversity. These are not stand replacing fires, though considerable amounts of biomass are burned and the conversion rate to biochemically stabilized black carbon (BC) is unknown. The primary mechanisms for long-term carbon sequestration in soil are mineral association, biochemical transformation (e.g. pyrogenesis) and physical protection. We quantified the recalcitrance of soil organic carbon (SOC) and its oxidation resistant fraction (SOCR; defined as residual SOC following H2O2 treatment and dilute HNO3 digestion) using radiocarbon dating (SOC and SOCR) and benzene polycarboxylic acids (BPCA) as molecular markers for polyaromatic C associated with BC. Mineral stabilized C is largely represented by SOCR contents and BC by total BPCA contents. Soils were collected by depth (0-10, 10-20, 20-50, 50-100 cm) at 14 managed LLP stands in Louisiana (LA), Georgia (GA) and North Carolina (NC) burned every two to five years. Across all sites, SOC and SOCR contents declined with soil depth, though SOCR:SOC increased with depth (0.13, 0.15, 0.22, 0.31). SOCR was more 14C depleted than SOC and Δ14C values became more negative with soil depth (SOCR: -195, -318, -458, -553 vs. SOC 23, -39, -156, -334), indicating that SOCR had a much longer mean residence time. The Δ14C values correspond to mean ages of SOCR ranging from 1777 to 6969 years and of SOC from 84 to 3319 years. We obtained very low BPCA yield from SOCR, and it is unclear whether BC was absent or not accessible with the BPCA method. Preliminary analysis of total BPCA (bulk soil) indicates interactions between soil series and depth. Total BPCA concentration of SOC in the upper 10 cm was 136 g kg-1 C in LA and more than six times the concentration in GA and NC. On deep sands in NC, the highest BPCA concentration of SOC was found at 50-100 cm (68 g kg-1 C), which was three times higher than in GA or LA at that depth. Variation in bulk soil Δ14C was largely explained by SOCR:SOC and soil depth (combined partial R2 0.84), while BPCA:SOC contributed an additional 0.02 partial R2. This implies that SOC recalcitrance is largely controlled by mineral association versus pyrogenic transformation.

  18. Dependences of longleaf pine (Pinus palustris) natural reproduction on environments above ground

    Institute of Scientific and Technical Information of China (English)

    Qiuju Guo; Dexiang Wang; Xunru Ai; Lan Yao

    2014-01-01

    We studied relations between natural seedling reproduction and above ground environment in a longleaf pine ecosystem. Forty-eight 0.05 ha circular plots were sampled under single-tree selection, group-tree selection and control stands in three main longleaf pine areas in south Alabama, USA. We measured six above-ground environment factors, viz. canopy closure, stand density, basal area, average tree height, understory cover and PAR under canopy. We employed forward, back-ward and stepwise selection regression to produce one model. Three main variables:canopy closure, stand density and basal area, were left in the model; light, PAR and understory cover were not incorporated into the model at the 0.10 significance level. Basal area was a positive pa-rameter, while canopy closure and stand density were negative parame-ters. Canopy closure was the main parameter in the model. The model proved to be meaningful, and has potential to provide useful guidance for future work.

  19. Photosynthetic consequences of phenotypic plasticity in response to submergence: Rumex palustris as a case study

    NARCIS (Netherlands)

    Mommer, L.; Pons, T.L.; Visser, E.J.W.

    2006-01-01

    Survival and growth of terrestrial plants is negatively affected by complete submergence. This is mainly the result of hampered gas exchange between plants and their environment, since gas diffusion is severely reduced in water compared with air, resulting in O2 deficits which limit aerobic respirat

  20. Study on Kinetic Parameters of Degradating Phenol by Photosynthetic Bacteria%光合细菌降解苯酚的动力学参数研究

    Institute of Scientific and Technical Information of China (English)

    刘宏芳

    2011-01-01

    苯酚是炼焦(油)、塑料、化工等行业生产过程中的主要污染物。随着经济的快速发展,各类含酚废水已经严重威胁着人类的生存环境。利用微生物处理含酚废水是一种经济有效且无二次污染的方法。本文主要研究了光合细菌-沼泽红假单胞菌降解含酚废水的动力学参数。实验结果表明,沼泽红假单胞菌对含酚废水具有很好的降解性能,正常状态下,该菌最大比生长速率μmax为8.00 mg/g.h,半速率常数Ks为247.92 mg/L,产率系数Y为5.88 mg/mg,内源呼吸系数Kd为0.29 d-1。%Phenol is the significant raw material or midst substance of coking plant,oil refining,plastic and medicine composing etc.More and more waste water containing phenol without treatment is threatening the environment heavily with the rapid development of economy.The method of microorganism biodegradation is economic and effective to treat with phenol waste water without second pollution.The kinetic parameters of the degradation of the phenol were studied with Rhodopseudomonas palustri.The experimental results show that Rhodopseudomonas palustris had excellent biodegradability for phenol wastewater.Under normal conditions,μmax is 8.00 mg/g·h,Ks is 247.92 mg/L,Y is 5.88 mg/mg and Kd is 0.29 d-1.

  1. 一种净化水质的复合生物修复系统%A composite bioremediation system for water purification

    Institute of Scientific and Technical Information of China (English)

    袁亚光; 张弛; 谢卫平; 赵福庚; 钦佩

    2012-01-01

    在实验室内利用人工模拟方法,选择水蕹菜(Ipomoea aquatica)、泥鳅(Misgurus anguillicaudatus)、沼泽红假单胞菌(Rhodopseudomonas palustris)为工程物种,构建一套水生经济植物-水生动物-微生物复合生物修复系统进行污水修复,研究该系统中动植物生物量及水质指标的变化.结果表明,在23 d的实验周期中,水体铵态氮(NH4+-N)下降96.5%,硝态氮(NO3--N)下降82.2%,总磷(TP)下降53.2%,化学需氧量(CODMn)下降24.5%.水蕹菜平均增重31.2%,泥鳅平均增重6.1%.这种复合的生物修复模式具有较好的经济效益与环境效益.%By the method of artificial simulation in laboratory, and with water spinach (Ipomoea aquatic), loach (Misgurus anguillkaudatus) , and a kind of pseudomonad (Rhodopseudomonas palustris) as the engineering species, a composite bioremediation system of aquatic economic plant - aquatic animal - microorganism was constructed to improve the water quality of sewage. The biomass of I. aquatic and M. anguillicaudatus in the system and the water quality indices were investigated. Within the 23 days experimental period, the nutrient concentrations in the sewage decreased significantly, with the removal rates of NH4+-N, NO3--N, TP, and CODMn being 96.5%, 82. 2%, 53. 2%, and 24.5%, respectively. Meanwhile, the biomass of I. aquatic and M. anguillicaudatus was averagely increased by 31.2% and 6. 1% , respectively. All the results suggested that this composite bioremediation model had good economic and environmental benefits.

  2. Die Übertragungsgenauigkeit von okklusalen Verhältnissen in den Artikulator durch verschiedene Registriermaterialien

    OpenAIRE

    Jansen, Stephan

    2007-01-01

    Im Rahmen der vorliegenden Untersuchung wurden verschiedene Registriermaterialien auf ihre Übertragungsgenauigkeit beziehungsweise Eignung in bezug auf eine Reproduktion von Kieferrelationsverhältnissen bei voll bezahnten Modellen geprüft. In einem ersten Versuchsteil wurden die verschiedenen Registriermaterialien (neben Wachsen und dem Abformgips außerdem Elastomere, und zwar bis auf Ramitec Penta® [einer Polyether-Bißregistrierpaste] ausschließlich schnellanbindende additionsvernetzende ...

  3. Schwellenwertbestimmung von Gewebeschädigungen durch Hochfrequenzfelder im MRT im Schweinemodell

    OpenAIRE

    Piaskowski, Markus Jan

    2012-01-01

    This experiment was performed in order to establish thresholds for muscle tissue damage induced by radiofrequency hyperthermia using cryostat-sections and temperature measurements. The measured variables were the specific absorption rate (SAR), the cumulative equivalent minutes at 43 ºC and the maximum temperature (T_max). To this end, 11 pigs of German Landrace were anesthesized intravenously (TIVA). Radiofrequency was applied as whole body SAR with 2,5-5,2 W/kg for 30-60 min in a birdca...

  4. Von Whorf zu Malotki - Eine Reise durch Hopi-Raum und Hopi-Zeit

    DEFF Research Database (Denmark)

    Robering, Klaus

    2014-01-01

    The article investigates the views of Whorf on the linguistic representation of space and time and their reception in German linguistics in the 1960s. Its first part summarizes both Whorf’s general conception of the relationship between language and cognition and his semantic analysis of the way...... how the Hopi language represents temporal and spatial matters. The Hopi conception of space and time (as conceived by Whorf) is contrasted with the views of classical and relativistic physics. The third part is devoted to the reception of Whorf’s views in German linguistics in the 1960s. A brief...... of Helmut Gipper, who in turn was a disciple of Weisgerber) are summarized and compared with Whorf’s original views. The article ends with a brief comment upon the treatment of space and time in Neo-Whorfianism....

  5. Zugang zu neuen FuE-Konzepten durch innovative Verfahren der Pflanzenphänotypisierung

    Directory of Open Access Journals (Sweden)

    Altmann, Thomas

    2011-10-01

    Full Text Available The development and use of automated procedures of non- or minimal invasive monitoring of plant traits and features opens new opportunities for the identification of genetic factors determining plant characteristics and performance parameters, for the investigation of environmental effects on trait expression, for the selection of individuals or lines with preferred features from large populations, for the optimization of plant production systems, and for the recognition of novel uses of plants. This is achieved through development and application of precise and unbiased measurement procedures (not influenced by human perception, which (ideally are amenable to high-throughput approaches. They enable more accurate and much larger scale investigations or screenings than hitherto possible, they allow the detection of features that previously could not be assessed, and they offer access to monitoring and quantification of dynamic processes through repeated measurements over time. To this end, (novel sensor and detection systems have to be developed and an implemented, automation procedures have to be adapted and established, corresponding facilities have to be constructed and installed, which support monitoring and/or simulation of certain environmental parameters (in addition to the measurement of plant features and effective data analysis tools have to be developed and implemented.

  6. Von Whorf zu Malotki - Eine Reise durch Hopi-Raum und Hopi-Zeit

    DEFF Research Database (Denmark)

    Robering, Klaus

    2014-01-01

    discussion of classical German philosophy of language (Hamann, Herder, Humboldt) prepares the stage for relating Whorf’s views to those of Leo Weisgerber’s ‘Inhaltbezogene Grammatik’, then the dominant trend in German linguistics. The studies of ‘Hopi space’ and ‘Hopi time’, by Ekkehart Malotki (a disciple...

  7. Zweiwegintegration durch zweisprachige Bildung? Ergebnisse aus der Staatlichen Europa-Schule Berlin

    Science.gov (United States)

    Meier, Gabriela

    2012-06-01

    While there is no simple recipe of how to respond to the multitude of languages present in many European schools, this article presents a promising alternative to monolingual education. The focus is on Staatliche Europa-Schule Berlin (SESB), a two-way immersion (TWI) model that unites children whose mother tongue is German with children whose mother tongue is another locally spoken language in one class and teaches them together in two languages. Thus in this model, offered by 17 primary schools and 13 secondary schools in Berlin, pupils learn in two languages from and with each other. Based on a largely quantitative, quasi-experimental study with 603 students, evidence is provided that there are a number of peace-linguistic benefits that can promote two-way social integration, besides fostering personal and societal multilingualism. This suggests that TWI education as practised in Berlin could serve as an educational model for other multilingual parts of Europe.

  8. Renewable energies supported by GIS and land management; Erneuerbare Energien unterstuetzt durch GIS und Landmanagement

    Energy Technology Data Exchange (ETDEWEB)

    Klaerle, Martina (ed.)

    2012-11-01

    The author of the book under consideration reports on the support of renewable energy sources by Geographical Information Systems (GIS) and land management. The first part of this book is addressed to actual land political and planning legal fundamentals of the energy policy turnaround as well as on future developments of the planning instruments. The second part of this book presents GIS based tools and concrete application examples which are very valuable for regional authorities in the implementation of the energy policy turnaround: solar plant cadastre, holistic potential analysis for all forms of renewable energy systems, visibility studies, flexible power grids and so forth.

  9. Durch intrinsische defekte induzierte uphill-diffusion von Ag und Cu in CdTe

    CERN Document Server

    Wagner, Frank

    In the framework of the present thesis, the diffusion of Ag in CdTe was investigated by the radiotracer $^{111}$Ag. Thereby the focus was on the possibility to create a Ag flux from regions of low Ag concentration to regions of high Ag concentration (uphill diffusion). The experimentally observed diffusion profiles are explained in the framework of a thermodynamic diffusion model, taking into account the defect charge state and the defect interaction. The distribution of the charged defects produces a electric field, which leads to a drift of the charged defects. The experimental data are well explained assuming that Ag is incorporated interstitially and ionized (Agi$^{+}$). The Agi$^{+}$ concentration then reflects the profile of the Fermi level, which again is determined by the intrinsic defect distribution or, more precisely, the deviation from stoichiometry. On the basis of the experimental data it is possible to gather information on the thermodynamic properties of extrinsic as well as intrinsic defects....

  10. Degradation of petroleum hydrocarbons through microorganisms. Abbau von Mineraloel-Kohlenwasserstoffen durch Mikroorganismen

    Energy Technology Data Exchange (ETDEWEB)

    Wachtendonk, D. v. (Lebensmittel- und chemisches Untersuchungsamt Aachen (Germany)); Klapp, K.U.

    1994-12-01

    Before organic decontaminations by microorganisms are carried through the properties and behaviour of the soils to be cleaned up should be known or established in order to permit rapid and through cleanup. Relevant factors are, especially, the particle size and homogenicity of the soil, which may be optimized by different additives and techniques. When these factors are known and after addition of appropriate amounts of nutrients, ''on-site'' microbiologicaal soil cleanup is to be given preference over any other method of decontamination. (orig.)

  11. Vorhersage des Krankheitsverlaufes von leichten kognitiven Beeinträchtigungen durch automatisierte MRT Morphometrie

    Science.gov (United States)

    Fritzsche, Klaus H.; Schlindwein, Sarah; Stieltjes, Bram; Essig, Marco; Meinzer, Hans-Peter

    Die leichte kognitive Beeinträchtigung (LKB) gilt als Anzeichen für ein erhöhtes Risiko der Entwicklung einer Alzheimerdemenz. Eine fundierte klinische Prognose für den Krankheitsverlauf kann aber bis dato nicht gegeben werden. Das Ziel dieser Arbeit besteht darin, eine möglichst präzise Vorhersage mittels automatisierter Morphometrie des Hippokampus im MRT-Bild zu treffen. In einer Studie mit 18 Probanden mit LKB wurde eine Prädiktionsgenauigkeit für die Entwicklung einer späteren Demenz von 83.3% erzielt. Eine manuelle Vergleichsmethode erreichte mit 55.6% Trefferquote keine signifikante Vorhersagegenauigkeit. Das automatische Verfahren erfüllt viele wichtige Voraussetzungen für den routinemäßigen klinischen Einsatz mit dem Potential, die klinische Vorhersage des Krankheitsverlaufes bei der LKB zu verbessern.

  12. More effectivity in the gas supply through network codes; Mehr Effektivitaet bei der Gasversorgung durch Netzkodizes

    Energy Technology Data Exchange (ETDEWEB)

    Kremer, Andreas [EVB Energy Solutions GmbH, Velbert (Germany). EVB Akademie

    2013-02-15

    Already in the year 2003, the European Commission has regulated the complete opening of the market by means of the regulation 2003/55/EG in order to develop the European Single Market positively. Practically, in Germany industrial customers can freely choose their gas suppliers since 1st July, 2004, while private customers can choose their gas suppliers since 1st July, 2007. The regulation 2003/55/EG is valid for the gas market inclusive natural gas, liquefied natural gas (LNG), biogas, gas from biomass and any other types of gas which can be transported through the natural gas distribution system.

  13. Safety margins and retrofit. The technical calculation perspective; Sicherheitsmargen durch Nachruestung aus Sicht der technischen Berechnung

    Energy Technology Data Exchange (ETDEWEB)

    Daichendt, Matthias [Kraftanlagen Heidelberg GmbH, Heidelberg (Germany). Systemtechnik - Technische Berechnungen

    2016-01-15

    Safety margins are an essential factor of the safety philosophy for nuclear power plants. They support to cover future requirements even today. The basic safety concept is one key topic as also aspects of process engineering, the dimensioning and mechanical analysis of systems and ageing management. Calculations with today's capabilities are an integral part of the determination of safety margins. They can be used to analyse and to assess retrofit measures.

  14. Signatures of dynamics in charge transport through organic molecules; Dynamisches Verhalten beim Ladungstransport durch organische Molekuele

    Energy Technology Data Exchange (ETDEWEB)

    Secker, Daniel

    2008-06-03

    The aim of the thesis at hand was to investigate dynamical behaviour in charge transport through organic molecules experimentally with the help of the mechanically controlled break junction (MCBJ) technique. the thesis concentrates on the complex interaction between the molecular contact configuration and the electronic structure. it is shown that by variation of the electrode distance and so by a manipulation of the molecule and contact configuration the electronic structure as well as the coupling between the molecule and the electrodes is affected. The latter statement is an additional hint how closely I-V-characteristics depend on the molecular contact configuration. Depending on the applied voltage and so the electric field there are two different configurations preferred by the molecular contact. A potential barrier between these two states is the origin of the hysteresis. A central part of the thesis is dealing with measurements of the current noise. Finally it can be concluded that the detailed discussion reveals the strong effect of dynamical interactions between the atomic configuration of the molecular contact and the electronic structure on the charge transport in single molecule junctions. (orig.)

  15. Bone alterations by stress in athletes. Schaedigung des Knochens durch Ueberlastung bei Leistungssportlern

    Energy Technology Data Exchange (ETDEWEB)

    Doege, H. (Bezirkskrankenhaus ' Friedrich Wolf' , Abt. fuer Nuklearmedizin, Chemnitz (Germany))

    1990-01-01

    This report describes our experiences with the bone imaging in athletes. We studied 10 athletes and 10 other patients with spondylolisthesis of the lumbar spine and 16 athletes with suspicion of alterations of extremities. An increased uptake of this radiopharmaceutical was detected in six of 10 athletes with spondylolisthesis caused probably by stress fracture. Bone scans were negative in seven of 16 athletes with suspicion of lesion of extremities. In the remaining 9 patients scans were abnormal and showed periosteal injuries, epiphyseal alteration, joint abnormalities, tibial stress fractures and couvert fracture. It was also abnormal in bone injuries not evident in radiography. (orig.).

  16. Climate protection by reducing cooling demands in buildings; Klimaschutz durch Reduzierung des Energiebedarfs fuer Gebaeudekuehlung

    Energy Technology Data Exchange (ETDEWEB)

    Bettgenhaeuser, Kjell; Boermans, Thomas; Offermann, Markus; Krechting, Anja; Becker, Daniel [Ecofys Germany GmbH, Koeln (Germany)

    2011-06-15

    The aim of this study is to conduct estimation on the potential reduction in electricity demand from cooling appliances in buildings in Germany. Current electricity demand and greenhouse-gas emissions will be investigated through desk research for residential and non-residential buildings. Based on building simulations, conventional, alternative and renewable technologies will be compared for different reference buildings. An economic and environmental assessment will evaluate the technologies per reference building in further detail. The main result will be an estimation of the potential energy demand reduction for the alternative/ regenerative technologies in the building stock. This will be based on the conditioned floor area and retrofit rates per system. Furthermore, the influence of cooling in buildings on energy demand will be annotated. Barriers in the reduction of energy demand will be described possible actions will be discussed along with types of policy instruments and consumer information. (orig.)

  17. Rising the exploitation of substrates by thermal digestate processing; Steigerung der Substratausnutzung durch thermische Gaerrestbehandlung

    Energy Technology Data Exchange (ETDEWEB)

    Seick, Ingolf; Gebhardt, Sebastian [Hochschule Magdeburg-Stendal (Germany). Fachbereich Wasser- und Kreislaufwirtschaft

    2013-10-01

    The paper introduces a new process for biogas plants to increase the utilization of substrate and to reduce the required feeding. By a thermal treatment of a partial flow of the digestate the gas yield is increased considerably, whereupon a recirculation is carried out into the fermentation process. The digestate taken from the secondary fermenter is separated to a liquid and a solid phase. The solid phase is treated thermally and led back into the main fermenter discontinuously. The discharge of a subset of the untreated digestate shall avoid accumulation of inert fractions. Batch fermentation tests with separated digestate of a typical biogas plant have shown an increase of the VS-specific gas and methane yields, e.g. of approx. 90% after a 10-minute treatment in a lab-scale high pressure autoclave at 170 C and 8 bar. Simulations point out that a long-term use of the process in biogas plants can be possible effectively. (orig.)

  18. Simulation of biogas production by adding biochar; Steigerung des Biogasertrages durch die Zugabe von Pflanzenkohle

    Energy Technology Data Exchange (ETDEWEB)

    Roedger, Jan-Markus; Ganagin, Waldemar; Krieg, Andreas; Roth, Christian; Loewen, Achim [HAWK Hildesheim/Holzminden/Goettingen, Goettingen (Germany). Fachgebiet Nachhaltige Energie- und Umwelttechnik (NEUTec)

    2013-09-15

    One instrument to withdraw carbon dioxide from the atmosphere is the so called Biochar. Through photosynthesis and a thermochemical biomass treatment (pyrolysis) the bound carbon will be stabilized and thereby stored permanently. The product (biochar) is intended to be mainly used as soil enhancer on arable land. Early analyses indicate no economic feasibility yet. Therefore new ways of utilization need to be identified along the agricultural value chain to generate additional earnings. One option is the application of biochar as an additive within the biogas process. Different international research institutes were able to measure an increase in biogas production due to the biochar addition. One reason might be accumulation of methanogenic bacteria as well as the increase of the surface area to improve the microbial functionality which leads to additional gas production. The study presented here investigated the optimal process stage within the fermentation process (main digester or secondary fermenter) to add the biochar and thereby improve the gas production. By adding biochar to the main digester an increase in gas production of approximately 9 % over a time span of 91 days was measured. To analyse the effect within the secondary fermenter two different approaches were tested. The effect of the surface area was assessed by comparing the influence of original biochar and large char particles. The original Biochar led to an increase of more than 13 % and the large particles even enhanced methane production by 24 % over 91 days. (orig.)

  19. "Neue Liebe, neues Leben" - Ein Goethe-Gedicht in der Vertonung durch Ludwig van Beethoven

    Directory of Open Access Journals (Sweden)

    Körndle, Franz

    2001-12-01

    Full Text Available Ludwig van Beethoven composed two versions of Goethe's poem „Neue Liebe, neues Leben" in 1798/99 and 1810 respectively. A comparison of both versions reveals a change in the composer's intention. At first in several passages he followed the more traditional technique of transferring single words of the text directly into musical figures. Later on he seemed more intent on expressing the general mood and eliminated some of the rather simplistic details in favour of a more subjective rendering of the poems content.

    [de] Ludwig van Beethoven vertonte das Goethe-Gedicht „Neue Liebe, neues Leben" in zwei Fassungen 1798/99 und 1810. Der Vergleich der beiden Fassungen ergibt, daß sich in der Zeit zwischen diesen beiden Fassungen die Vorstellungen des Komponisten von der musikalischen Umsetzung teilweise gewandelt haben müssen. Zunächst scheint Beethoven eher nach der traditionellen Méthode einer direkten Übertragung von Begriffen aus dem Text komponiert zu haben. Später war er dann aber eher an einer subjektiven Darstellung des Inhalts interessiert und drângte drängte zu einfach gerate Einzelheiten zugunsten der - nunmehr freilich recht differenziert gestalteten - Gesamtstimmung zurück.

  20. Thermische Charakterisierung selbstevakuierender Kryodämmmaterialien durch Ausfrieren von Kohlenstoffdioxid als Füllgas

    OpenAIRE

    Geisler, Matthias

    2011-01-01

    Gegenstand der vorliegenden Arbeit ist die thermische Charakterisierung des Einflusses von ausfrierenden Füllgasen auf die Wärmeleitfähigkeit von Wärmedämmstoffen für kryogene Anwendungen am Beispiel von Kohlenstoffdioxid. Im Allgemeinen wird mit dem Wärmedämmmaterial der Wärmeverlust eines Gegenstandes herabgesetzt, der eine Temperaturdifferenz im Vergleich zur Umgebung aufweist. Um die Gesamtwärmeleitfähigkeit eines Dämmstoffes bei kryogenen Temperaturen, d.h. < 200 K, zu minimieren, wird d...

  1. Multifunctional facades - design optimisation with CAD; Multifunktionale Fassaden - Effektive Auslegung durch CAD-Einsatz

    Energy Technology Data Exchange (ETDEWEB)

    Viotto, M. [Institut fuer Solare Energieversorgungstechnik (ISET), Kassel (Germany). Abt. fuer Anlagen- und Messtechnik

    1998-02-01

    The energetically optimal design of PV facades requires the consideration of many technical conditions (different elevations/orientations of modules; shading possibility; high and different module temperatures). It also requires the interface with architecture, civil and electrical engineering. To support this planning process, the software program PV-CAD was developed which rapidly calculates energy yield under conditions of inhomogeneous irradiance, shading, thermal behaviour of modules and electrical layout. PV-CAD is compatible with other civil and electrical engineering CAD programs and incorporates extensive databases (weather data; modules and inverter types). (orig.) [Deutsch] Fuer optimalen Energieertrag sind zahlreiche technische Randbedingungen bei der Auslegung einer Photovoltaik (PV)-Fassade zu beruecksichtigen (unterschiedliche Ausrichtungen/Neigungen der Module; moegliche Teilabschattung; hoehere und unterschiedliche Betriebstemperaturen). Installation und Betrieb einer PV-Fassade erfordern die Abstimmung zwischen Architektur, Bauwesen und Elektrotechnik. Zur Unterstuetzung der Planer wurde das Programm PV-CAD entwickelt, das den Energieertrag unter Beruecksichtigung von inhomogener Einstrahlung und Abschattung, Temperaturverhalten der Fassadenmodule sowie unterschiedlichen elektrischen Anlagenkonzepten schnell ermittelt; es fuegt sich nahtlos in CAD-Arbeitsumgebungen ein. (orig.)

  2. Positron emission tomography in presurgical diagnosis of partial epilepsies. Praeoperative Lokalisationsdiagnostik bei fokaler Epilepsie durch PET

    Energy Technology Data Exchange (ETDEWEB)

    Hajek, M.; Leenders, K.L. (Universitaetsspital Zurich (Switzerland). Neurologische Klinik Paul Scherrer Institut, Villigen (Switzerland)); Wieser, H.G. (Universitaetsspital Zurich (Switzerland). Neurologische Klinik)

    1992-06-01

    We present results of studies in which positron emission tomography was applied to the presurgical evaluation of epileptics. Emphasis is placed on results of PET studies with various tracers in partial epilepsies and on the use of PET in age-related epileptic syndromes in children. (orig.).

  3. Element pollution of exhaust aftertreatment systems by using biodiesel; Elementbelastungen von Abgasnachbehandlungssystemen durch Biodiesel

    Energy Technology Data Exchange (ETDEWEB)

    Stein, Hendrik; Wilharm, Thomas [ASG Analytik-Service Gesellschaft mbH, Neusaess (Germany); Winkler, Markus [DEUTZ AG, Koeln (Germany); Knuth, Hans-Walter

    2012-06-15

    Biodiesel is a particularly attractive fuel for agricultural machinery. However, the introduction of new emission standards has made the use of exhaust gas treatment systems in agricultural vehicles essential. The combination of biodiesel and exhaust gas treatment causes problems, because the biodiesel contains traces of inorganic elements. These turn into ash during the combustion process in the engine, which can result in permanent damage to the components of the exhaust gas treatment system. Deutz and ASG have investigated the impact of current grades of biodiesel on the systems in real-life operation. (orig.)

  4. Verminderte Tiefbrunnenergiebigkeit durch Versandung und Versinterung bei Veränderung der Grundwasserchemie

    Science.gov (United States)

    Knuth, Christoph; Wisotzky, Frank; Becke, Norbert

    2017-01-01

    In three deep wells of a water works in the Lower Rhine Valley (Germany) decreasing well yield has been observed. To investigate the reasons for this decline, solids analysis (microscopy, Cinorg measurements, XRD-, SEM-analysis) was completed and hydrogeochemical calculations were carried out. The samples examined from the bottom of the wells indicate both hydromechanical entry of sand from the aquifer into the well, and formation of incrustations in the form of calcite within the well. The water analysis results show that almost all measured ion concentrations have increased over the past 10 years, which indicates a general change of the hydrochemical conditions of the aquifer. Hydrogeochemical models suggest that oversaturation exists regarding the mineral phases iron hydroxide and calcium carbonate, and therefore the formation of incrustations is possible.

  5. Vorintegration durch Onlineberatung – Ein Projekt der Jugendmigrationsdienste (JMD: www.almanyayolu.org

    Directory of Open Access Journals (Sweden)

    Özcan Ülger

    2014-04-01

    Full Text Available The project pre-integration through online counseling supports young people from Turkey who have decided to migrate to Germany via a free online counseling platform. This is achieved together with the Goethe Institute, with whom most of the Young people have been in contact previous to their voyage to Germany. Through this cooperation, the migrants are being accompanied and supported even before their entrance to Germany. The project is co-financed by the European Integration Fund and the Federal Ministry of Family Affairs, Senior Citizens, Women and Youth (BMFSFJ. The service bureau for youth migration Services is responsible for Project management.

  6. Inhibition of hydrate formation by kinetic inhibitors. Literature study; Inhibierung von Erdgashydraten durch kinetische Inhibitoren. Literaturstudie

    Energy Technology Data Exchange (ETDEWEB)

    Eberhardt, E.; Meyn, V.; Rahimian, I. [Institut fuer Erdoel- und Erdgasforschung, Clausthal-Zellerfeld (Germany)

    2000-04-01

    The aim of this study was to represent the state-of-the art of the inhibition of gas hydrates. Corresponding to recent publications the kinetic inhibition was considered in particular. Special inhibitors were validated using a set of criteria derived from different experimental test methods. Best results were obtained by the application of terpolymer VC-713 especially in relation to nucleation and crystal growth, followed by PVCap (polyvinylcaprolactame) and THI (threshold hydrate inhibitor), the chemical structure of which is derived from the antifreeze glycopeptids of antarcitc winter flounder. (orig.) [German] Die vorliegende Literaturstudie gibt den derzeitigen Stand der Kenntnis zur Inhibierung von Gashydraten wieder. Entsprechend der neueren Literatur wird insbesondere auf die kinetische Inhibierung eingegangen. Zur Beurteilung der verschiedenen Inhibitoren werden Bewertungskriterien zur Validierung der mit unterschiedlichen Untersuchungsmethoden erzielten experimentellen Ergebnisse angegeben. Anhand dieser Vorgehensweise zeigte sich, dass mit dem Terpolymer VC-713 die besten Ergebnisse, insbesondere im Hinblick auf Keimbildung und Wachstum, erzielt werden konnten. Sehr gute Ergebnisse wurden auch mit dem Polyvinylcaprolactam (PVCap) und den aus den Antigefrierpeptiden der antarktischen Winterflunder abgeleiteten Threshold Hydrate Inhibitoren (THI) erhalten. (orig.)

  7. Natural gas production by fracking in Germany; Erdgasgewinnung durch Fracking in Deutschland

    Energy Technology Data Exchange (ETDEWEB)

    Muehlendahl, Karl Ernst v.; Otto, Matthias [Kinderumwelt gGmbH der Deutschen Akademie fuer Kinder- und Jugendmedizin, Osnabrueck (Germany)

    2012-11-01

    ExxonMobil is performing exploration tests in Lower Saxony concerning unconventional sources for natural gas production using hydraulic fracturing. The contribution is dealing with environmental policy related questions that affect public health and should be part of the information of physicians. The contribution covers information on the issues hydraulic fracturing, water consumption and application of chemicals, drilling accidents - blow-out, energy balance, legal questions, summarizing recommendations of neutral expert team, higher-ranking points of view with respect to environmental protection and compatibility. [German] Bei umweltpolitischen Fragen, die auch die Gesundheit der Bevoelkerung tangieren, sollten aerzte informiert sein, um ggf. sachkundig mitsprechen zu koennen. Der Bereitstellung einer notwendigen Basisinformation zu dem Thema Fracking, das in betroffenen Gebieten in Niedersachsen und im Muensterland viel diskutiert wird, dienen die nachfolgenden Ausfuehrungen.

  8. Experimenteller Nachweis der Symptomreduktion bei Patienten mit Kältekontakturtikaria durch Dosissteigerung von Bilastin

    OpenAIRE

    Spohr, Adrian

    2014-01-01

    Background: Cold contact urticaria (CCU) is characterized by itchy wheal and flare responses due to the release of histamine and other pro-inflammatory mediators after exposure to cold. The treatment of choice in CCU are non-sedating H1 antihistamines. If patients cannot be sufficiently controlled with standard dosages, the current EAACI/GA2LEN/EDF/WAO-guideline for the management of urticaria recommends high-dose H1 blockers up to 4 times the standard dose. Objective: Aim of the study is...

  9. Institutionalisierung durch Imitation? Die Europäisierung des Parlamentarismus in Mittel- und Osteuropa / Aron Buzogany

    Index Scriptorium Estoniae

    Buzogany, Aron

    2015-01-01

    Artikli teemaks on parlamentarismi euroopastumine Kesk- ja Ida-Euroopa riikides (sh Eesti), püüdes leida vastus küsimusele, kuidas on rahvusriikide parlamendid suutnud institutsionaalselt euroopastumise väljakutsele vastata?

  10. The influence of environmental factors on male fertility; Beeintraechtigung der maennlichen Fertilitaet durch Umweltfaktoren

    Energy Technology Data Exchange (ETDEWEB)

    Jung, A.; Schill, W.B. [Giessen Univ. (DE). Hautklinik (Zentrum fuer Dermatologie und Andrologie); Schuppe, H.C. [Duesseldorf Univ. (Germany). Hautklinik; Koehn, F.M. [Muenchen Univ. (Germany). Dermatologische Klinik und Poliklinik

    2000-07-01

    It is of increasing importance to assess the relationship between impaired male fertility and environmental factors. Due to the complex development and regulation of spermatogenesis, a variety of sites may be affected by exogenous noxae such as coffee, tobacco, alcohol, pharmaceuticals, professional agents, environmental chemicals and also physical factors. However, reliable findings in humans are only available for a few noxae. Unfortunately, results from animal experimental studies cannot be simply transferred. In the andrological practice, detailed history taking is of utmost importance for early recognition of possible exposure risks. Thorough analysis of the ejaculate is a prerequisite for detection and differentiation especially of disturbed spermatogenesis. Under aspects of occupational and environmental medicine, systematic search for data according to standardized criteria in exposed and unexposed men is imperative to find out potential reproductive toxins. (orig.) [German] Die Erfassung von Zusammenhaengen zwischen Einschraenkungen der maennlichen Fertilitaet und Umweltfaktoren gewinnt zunehmend an Bedeutung. Aufgrund der komplexen Entwicklung und Regulation der Spermatogenese ergeben sich vielfaeltige Angriffsmoeglichkeiten fuer exogene Noxen wie Genussmittel, Pharmaka, Arbeitsstoffe, Umweltchemikalien und auch physikalische Faktoren. Gesicherte Erkenntnisse liegen jedoch beim Menschen nur fuer wenige Noxen vor. Leider lassen sich tierexperimentelle Befunde nur begrenzt uebertragen. In der andrologischen Praxis kommt einer ausfuehrlichen Anamnese ein wichtiger Stellenwert zu, um moegliche Expositionsrisiken fruehzeitig erfassen zu koennen. Detaillierte Ejakulatanalysen sind hier zur Erfassung und Differenzierung, insbesonders von Spermatogenesestoerungen, unerlaesslich. Unter arbeits- und umweltmedizinischen Gesichtspunkten sind systematische Datenerhebungen nach standardisierten Kriterien bei exponierten und nicht exponierten Maennern unerlaesslich, um potentielle Reproduktionstoxine fruehzeitig erfassen zu koennen. (orig.)

  11. Modulation systemischer Chemokinspiegel durch rekombinantes Interferon-beta bei Patienten mit multipler Sklerose

    OpenAIRE

    Merzyn, Cornelia

    2009-01-01

    Multiple Sklerose (MS) ist eine chronisch-entzündliche Erkrankung des Zentralen Nervensystems mit deutlich ausgeprägten Autoimmunphänomenen. Das derzeit meistverwendete Therapeutikum zur Sekundärprophylaxe von Krankheitsschüben ist rekombinantes Interferon-β (IFN-β). Wirk- und Nebenwirkungsmechanismen des Medikaments werden bisher nur partiell verstanden. In der Pathogenese der MS spielt eine Familie chemotaktisch wirksamer Zytokine, der Chemokine, eine entscheidende Rolle. Ziel die...

  12. Der Erwerb der Verbflexion durch thailändische Immigrantinnen in der Schweiz. Eine Bestandaufnahme.

    Directory of Open Access Journals (Sweden)

    Attaviriyanupap, Korakoch

    2006-01-01

    Full Text Available This article focuses on the acquisition of German verb inflection by native speakers of Thai, an isolated language which has no concept of inflection at all. The acquisition process of German verb morphology is analyzed based on all the verb inflectional affixes found in the corpus consisting of spontaneous utterances in Standard German produced by16 female immigrants living in German-speaking Switzerland. It aims to find out a systematic acquisition order of verb inflectional morphemes and the explanation to this sequence, especially to answer the following four questions: 1 What is the acquisition order of verb inflectional morphemes found in this group of informants 2 Are there any differences between the acquisition of finite and that of non-finite verbs? 3 Are there any differences in the verb morphology acquisition of different types of verbs? 4 Does the acquisition of verb inflection by these informants share more similiarities with the instructed or with the natural acquisition of German as a second language?

  13. Modulation der Blutfette durch körperliche Aktivität

    Directory of Open Access Journals (Sweden)

    2004-01-01

    Full Text Available Gesteigerte körperliche Aktivität vermindert das kardiovaskuläre Risiko und klassische Risikofaktoren und ist ein zentraler Angriffspunkt für therapeutische Lebensstilmodifikationen. Regelmäßiges körperliches Training führt zu signifikanten Verbesserungen im Lipidprofil: einer Steigerung der Serumkonzentration des HDL-Cholesterins, einer Reduktion der Triglyzeride und einer Zunahme der LDL- sowie der HDL-Partikeldurchmesser. Aktivitätsänderungen der Schlüsselproteine Lipoproteinlipase, Cholesterinester-Transfer-Protein und Lecithin- Cholesterin-Acyltransferase sind wesentliche Mechanismen hinter diesen quantitativen und qualitativen Veränderungen der Lipide und Lipoproteine. Das Ausmaß des Trainings beeinflußt die Lipide mehr als die Trainingsintensität, was vom gesundheitlichen Aspekt von besonderer Bedeutung ist. Ein Training 3- bis 4mal pro Woche bei moderater Intensität über jeweils 30–45 Minuten scheint ausreichend. Der positive Effekt auf das Lipidprofil konnte sowohl bei Frauen als auch bei Männern, bei jüngeren und bei älteren Menschen sowie in der Primär- und Sekundärprävention nachgewiesen werden. Der alleinige Effekt körperlicher Aktivität auf das Lipidprofil reicht insbesondere in der Sekundärprävention nicht aus, um die Zielwerte – vor allem für das LDL-Cholesterin – zu erreichen. Hier muß ein multimodaler Ansatz einschließlich medikamentöser Therapie gewählt werden.

  14. Persistent environmental pollutants. Detoxification by means of fungi; Persistente Umweltschadstoffe. Detoxifizierung durch Pilze

    Energy Technology Data Exchange (ETDEWEB)

    Sietmann, Rabea; Schauer, Frieder [Ernst-Moritz-Arndt-Universitaet, Greifswald (Germany). Inst. fuer Mikrobiologie

    2010-01-15

    Microorganisms either may use environmental pollutants as a source of carbon or attack pollutants oxidatively in the presence of a growth substrate. Furthermore, accumulated toxic products partly can be removed from a cell and oxidized or mineralized by microorganisms. The structural chemical analysis of the accumulated metabolites is performed by means of high-performance liquid chromatography (HPLC), gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). This analysis supplies information on the mechanism of degradation of the environmental pollutants. Under this aspect, the authors of the contribution under consideration report on the use of fungi (aspergillus, candida, cunninghamella, fusarium, mucor, paecilomyces, penicillium, pycnoporus, trametes, trichosporon) in the degradation of biphenyl and its derivatives. The following mechanisms of degradation are discussed: Primary oxidation, hydroxylation, ring scission, formation of conjugates, oligomerisation and dehalogenation.

  15. Spinal trauma: first aid from cross-sectional imaging; Trauma der Wirbelsaeule: erste Hilfe durch Schnittbildverfahren

    Energy Technology Data Exchange (ETDEWEB)

    Schueller, G.; Schueller-Weidekamm, C. [Emergency Radiology Schueller, Neerach (Switzerland)

    2014-09-15

    The diagnosis of the traumatized spine is one of the key issues for trauma radiologists. The cross-sectional imaging procedures, computed tomography (CT) and magnetic resonance imaging (MRI) are the essential methods in spinal trauma radiology. These modalities are of great help in accurately assessing injury patterns and extent and in providing indications of patient outcome. In contrast to cross-sectional imaging, radiography has a role in the evaluation of minor spinal trauma only. It is generally accepted that trauma radiologists do not use typical classifications to evaluate the spine partly because such an ideal classification system does not yet exist. Not least because of this classification difficulty, eponyms and synonyms are widely used to describe traumatology of the spine as a high level of specific information is included in these various terms. The members of the trauma team should be aware of the strengths and limitations of the methods used in the assessment of the spine. This article provides a brief outline of fundamental knowledge about the diagnosis of spinal trauma. (orig.) [German] Die Beurteilung der verletzten Wirbelsaeule nimmt fuer Traumaradiologen eine zentrale Stellung ein. Die Schnittbildverfahren CT und MRT sind ihre wesentlichen Arbeitsmethoden. Sie helfen dabei, schnell und mit hoher Zuverlaessigkeit Aussagen ueber Art und Ausmass von Verletzungen zu treffen sowie Hinweise auf die Prognose der Patienten zu geben. Die Projektionsradiographie hat ihre Bedeutung lediglich in der Diagnostik des Bagatelltraumas und ist in ihrer Aussagekraft auch dort nicht unumstritten. Traumaradiologen bedienen sich nicht ausschliesslich typischer Klassifikationen des Wirbelsaeulentraumas, z. T. auch deshalb, da es die ideale Klassifikation aus heutiger Sicht nicht gibt. Vielmehr ist es wichtig, auch ueber Eponyme und Synonyme Bescheid zu wissen, da sie ein hohes Mass an spezifischen Informationen der spinalen Verletzungen verinnerlichen. Alle Mitglieder eines Traumateams sollen sich der Staerken und Schwaechen der verwendeten radiologischen Techniken bei der Beurteilung des Wirbelsaeulentraumas bewusst sein. Dieser Artikel gibt einen kurzen Abriss ueber die wesentlichen diagnostischen Kenntnisse der traumatisierten Wirbelsaeule. (orig.)

  16. Visualisierung analoger Schaltungen durch 3-D Animation von transienten SPICE-Simulationen

    Directory of Open Access Journals (Sweden)

    J. Becker

    2007-06-01

    Full Text Available Für das Zeichnen analoger Schaltpläne wird oft versucht, die Potentialverteilung in der entsprechenden Schaltung auszunutzen, um eine Platzierung der Bauteile nach abfallendem Potential vorzunehmen. Mit Hilfe von Computerunterstützung gelingt es, eine verallgemeinerte dreidimensionale Platzierungsstrategie anzuwenden, die allein auf Grund der Potentialwerte einer Schaltung die automatische Generierung einer technisch exakten Potentialdarstellung erlaubt. Somit ist es möglich, die Ergebnisse von transienten SPICE-Simulationen in jedem Zeitschritt darzustellen und eine Animation des zeitlichen Verhaltens zu erzeugen. Die Umsetzung dieser Methode zur Einbettung in eine webbasierte Lern – und Arbeitsplattform wird im Folgenden erläutert.

  17. EU-Erweiterung: Herausforderung durch die Zuwanderung aus den neuen Mitgliedsländern

    OpenAIRE

    Georges de Ménil

    2004-01-01

    In seiner Rede beim 3. Munich Economic Summit am 18. Juni 2004 setzte sich Prof. Georges de Ménil, Ecole des Hautes Etudes en Sciences Sociales, Paris, und Stern School, New York University, mit der neuen EU-Freizügigkeitsrichtlinie auseinander. Da seiner Ansicht nach die Zuwanderung aus den neuen Mitgliedsländern langfristig für die Gastländer von großem Nutzen sein wird, sollte man sie nicht behindern. Die Poliik sollte vielmehr mit Reformen des Arbeitsmarktes und der Sozialsysteme reagiere...

  18. Unterstützung naturwissenschaftlicher Grundbildung durch Schulklassenbesuche in naturwissenschaftlich-technischen Museen

    OpenAIRE

    Neubauer, Katrin

    2016-01-01

    Die Dissertation vergleicht systematisch und theoriebasiert die motivationalen und kognitiven Wirkungen unterschiedlicher Besuchsformen von Schulklassenbesuchen im naturwissenschaftlich-technischen Museum. Dabei werden sowohl motivationale und kognitive Lernergebnisse als auch Lernprozesse der Schüler in Abhängigkeit der unterschiedlichen Besuchsformen untersucht. Die Ergebnisse verdeutlichen das Potenzial von Museen für die Interessensentwicklung und den Wissenserwerb und zeigen relevante le...

  19. Lightweight construction through miniaturization of components and systems; Leichtbau durch Miniaturisierung von Komponenten, Bauteilen und Systemen

    Energy Technology Data Exchange (ETDEWEB)

    Michel, B. [Fraunhofer-Institut fuer Zuverlaessigkeit und Mikrointegration (IZM), Berlin (Germany)

    2000-07-01

    The paper deals with advantages and problems concerning the application of microsystem technology in the field of lightweight construction. Miniaturization by means of advanced microtechnologies and lightweight materials will become more and more important also in lightweight construction, if it is possible to solve the questions of reliability and the problem of the costs as well. Composite materials and material compounds are playing a decisive role for further progress of lightweight construction. It is necessary to accomplish the compatibility requirements between the different materials on the 'small scale level' in a sufficient way. Above all the problems concerned with the so-called 'thermal misfit' have to be taken into account arising from the microdeformations of the different materials with different material parameters (e.g. CTE). New requirements arise which are related to research activities in different fields (e.g. materials research, materials mechanics, design, and various technologies as well). The author presents some practical applications from different fields, e.g. automotives, information and communication technology etc. showing as well the resources and the existing limits for the further progress of microsystem technology in lightweight construction. (orig.) [German] Leichtbau und Miniaturisierung, Mikrosystemtechnik etc. scheinen auf den ersten Blick kaum miteinander in Beziehung zu stehen. Dieser Eindruck ist aber falsch. Der generelle Trend zur Miniaturisierung betrifft nicht nur die Mikroelektronik - wo er naturgemaess betraechtliche Auswirkungen hat - sondern nahezu alle Bereiche der modernen Technik. Die Mikrotechnologien werden zunehmend wichtig fuer alle Technologiebereiche, die Mikrowerkstoffe ('Micro Materials'), d.h. diejenigen Werkstoffe, die in besonderem Masse die Miniaturisierung vorantreiben sollen, sind nicht nur im Bereich der Mikrosystemtechnik von Bedeutung, sondern haben auch im Bereich der Systeme ihre z.T. sehr breiten Einsatzgebiete. Eine der wichtigsten Triebkraefte fuer die Miniaturisierung ist die Frage der Kosten. Das trifft in gleichem Masse auch auf den Leichtbau zu. Die damit im Zusammenhang stehenden Fragen der Gewichts- bzw. Volumenreduzierung sind von aehnlicher Tragweite in beiden Bereichen. Erfahrungen des Leichtbaus mit Verbundwerkstoffen (Faserverbunde, Schichtverbunde) sind sehr wichtig fuer Anwendungen im Bereich der Mikrosystemtechnik, z.B. im Bereich der Aufbau- und Verbindungstechniken, im Packaging usw. Vom Einsatz der sogenannten 'Hochtemperaturelektronik' erwartet man sich in den naechsten Jahren deutliche Fortschritte auch in der Mikrosystemtechnik, in der Mikromechatronik, vor allem im Automobilbau. Dabei ist man schon froh, den Bereich oberhalb von 150 C erschliessen zu koennen. Umfassende Konzepte der Thermomechanischen Vertraeglichkeit (TMV) - oder im Englischen TMC (Thermo-Mechanical Compatibility) werden nicht minder wichtig als die bekannten EMV(EMC)-Vertraeglichkeiten in der Elektrotechnik. Der Autor wird im folgenden auf einige Probleme der Zuverlaessigkeit noch naeher eingehen. (orig.)

  20. Energy management through building automation. Fundamentals - Technologies - Applications; Energiemanagement durch Gebaeudeautomation. Grundlagen - Technologien - Anwendungen

    Energy Technology Data Exchange (ETDEWEB)

    Aschendorf, Bernd [Fachhochschule Dortmund (Germany). Fachbereich Informations- und Elektrotechnik

    2014-02-01

    The books available in the market consider only the use of individual buildings bus systems, but not to compare with each other with respect to cost-benefit and applicability. In this book, a total of 40 different systems, such as radio bus systems, PEHA-PHC, EIB, LCN, LON, PLC systems, investigated for their possible use in the various categories of buildings. The comparison refers to all levels of the automation pyramid from fieldbus, to automation to the control level and considers in particular the usability for SmartMetering-based energy management. [German] Die am Markt vorhandenen Buecher betrachten lediglich den Einsatz einzelner Gebaeudebussysteme, nicht jedoch den Vergleich untereinander mit Bezug auf Kosten/Nutzen und Anwendbarkeit. In diesem Buch werden insgesamt 40 verschiedene Systeme, wie z.B. Funkbussysteme, PEHA-PHC, EIB-, LCN-, LON-, SPS-Systeme, auf deren Einsatzmoeglichkeit in den verschiedenen Gebaeudekategorien untersucht. Der Vergleich bezieht sich auf alle Ebenen der Automatisierungspyramide vom Feldbus ueber die Automatisierung bis zur Leitebene und betrachtet insbesondere auch die Verwendbarkeit fuer smartMetering-basiertes Energiemanagement.

  1. Air conditioning using waste heat from fuel cells; Konzeptstudie: Klimatisierung durch Abwaermenutzung aus Brennstoffzellen - Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Gantenbein, P.; Luzzi, A.; Spirig, M. [Hochschule fuer Technik Rapperswil (HSR), Institut fuer Solartechnik (SPF), Rapperswil (Switzerland); Schuler, A.; Nerlich, V. [Hexis AG, Winterthur (Switzerland)

    2007-07-01

    This concept study for the Swiss Federal Office of Energy (SFOE) reports on work done at the University of Applied Sciences in Rapperswil, Switzerland on possibilities of using the waste heat from fuel cell stacks to provide heating and, in the summertime, cooling using an absorption refrigeration system. The study evaluates the technical, economical and market-relevant aspects of such systems. The methods used in making comparisons with conventional reference systems, including reviews of existing information and expert questioning, are discussed. The results obtained are presented and the results of sensitivity analyses are discussed. These include electricity feed-in tariffs and gas prices, pay-back times, capital interest rates, etc. Further, barriers encountered such as patents and other market hindrances are discussed. The report is completed with a comprehensive appendix.

  2. Biogenic methane from hydrothermal gasification of biomass; Biogenes Methan durch hydrothermale Vergasung von Biomasse

    Energy Technology Data Exchange (ETDEWEB)

    Schubert, M.; Vogel, F.

    2007-09-15

    This final report for the Swiss Federal Office of Energy (SFOE) reports on work done in the area of gasification of biomass. The use of dung, manure and sewage sludge as sources of energy is described and discussed. Hydrothermal gasification is proposed as an alternative to conventional gas-phase processes. The aim of the project in this respect is discussed. Here, a catalytic process that demonstrates the gasification of wet biomass to synthetic natural gas (SNG) in a continuously operating plant on a laboratory scale is being looked at. Difficulties encountered in preliminary tests are discussed. Long-term catalyst stability and the installations for the demonstration of the process are discussed, and gasification tests with ethanol are commented on.

  3. Challenges of future power transmission grids; Herausforderungen durch die Netze der Zukunft

    Energy Technology Data Exchange (ETDEWEB)

    Grasmann, Stefan

    2012-06-06

    The energy market is facing many innovations in the micro scale. Micro-power plants are gaining ground. On the other hand, the reliability of electric power supply is under discussion. The contribution attempts to thrown some light on the effects of current trends on the solar industry.

  4. Verminderte Tiefbrunnenergiebigkeit durch Versandung und Versinterung bei Veränderung der Grundwasserchemie

    Science.gov (United States)

    Knuth, Christoph; Wisotzky, Frank; Becke, Norbert

    2017-03-01

    In three deep wells of a water works in the Lower Rhine Valley (Germany) decreasing well yield has been observed. To investigate the reasons for this decline, solids analysis (microscopy, Cinorg measurements, XRD-, SEM-analysis) was completed and hydrogeochemical calculations were carried out. The samples examined from the bottom of the wells indicate both hydromechanical entry of sand from the aquifer into the well, and formation of incrustations in the form of calcite within the well. The water analysis results show that almost all measured ion concentrations have increased over the past 10 years, which indicates a general change of the hydrochemical conditions of the aquifer. Hydrogeochemical models suggest that oversaturation exists regarding the mineral phases iron hydroxide and calcium carbonate, and therefore the formation of incrustations is possible.

  5. Mikrobieller Abbau von 14 C-markiertem Benzo[a]pyren durch PAK-adaptierte Bakterienmischkulturen

    OpenAIRE

    Schwiening, Susanne

    2000-01-01

    In the last years the biodegradation of polycylic aromatic hydrocarbons (PAH) for the bioremediation of polluted soils was studied. Important for a successfull remediation is the degradation of the high molecular weight PAH, a major class of carcinogenic and persistent organic compounds. So the aim of this work was to study the biodegradation of the 5-ring benzo[a]pyrene (BaP) as a priority pollutant of the high molecular weight PAH. With the use of 14C labeled BaP the degradation could be qu...

  6. "Fidelity" und die verbesserte Unterstützung audiovisueller Analysen durch Software

    OpenAIRE

    Silver, Christina; Patashnick, Jennifer

    2011-01-01

    Seit den letzten 30 Jahren wurden sukzessive Programme zur Unterstützung qualitativer Datenanalysen entwickelt. Allerdings ist ihre Nutzung noch immer nicht sehr verbreitet. Zugleich hat sich qualitative Forschung selbst verändert: von Projekten, die kleine, textbasierte Datensets nutzten hin zu Projekten, in denen große Mengen an multimedialen Daten oder unterschiedlichste Datensorten erhoben, verwaltet und organisiert werden. Für die Softwareentwicklung gingen mit diesen Veränderungen eini...

  7. Infarkte an mesenterischen Lymphknoten und intestinale Gangrän durch Ascaris lumbricoides bei einem Kind

    OpenAIRE

    Bhutia, Kincho Lhasong; Dey, Subhajeet; Singh, Varun; Gupta, Amlan

    2011-01-01

    Ascaris lumbricoides or round worm infestation is quite common in the developing world. It affects all age groups but is more common in children. Most of the cases remain asymptomatic. The usual presentation is an intestinal obstruction. The physicians should be aware of this condition and consider it in the differential diagnosis when faced with such a case. The rare fatal complications include bleeding, perforation and gangrene. In Entwicklungsländern ist der Befall mit Ascaris lumbricoi...

  8. Determinanten des Verhaltens : Verhaltensprädiktion durch eine Weiterentwicklung der Theory of Planned Behavior

    OpenAIRE

    Hilkenmeier, Frederic; van Treeck, Joost

    2007-01-01

    "Obwohl die Theory of Planned Behavior eines der einflussreichsten Modelle der Sozialpsychologie ist, konnte die Wirkweise ihrer Komponenten bis heute nicht eindeutig geklärt werden. Die theoretisch naheliegende und oft publizierte Vermutung, zwischen Verhaltensabsicht und Kontrollmöglichkeit bestehe eine Interaktion, ist bisher nie eindeutig nachgewiesen worden. Diese Studie entwickelt ein Modell, das den Gedanken der Interaktivität gezielt in die Theorie integriert. Die Bestimmung von Kennw...

  9. Damage analysis. Product improvement through damage analysis; Schadensanalyse. Produktverbesserung durch Schadensanalyse

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2011-07-01

    Within the 37th VDI annual meeting from 19th to 20th May, 2011 in Wuerzburg (Federal Republic of Germany) the following lectures and posters were presented: (1) Malpractice of material processing and heat processing of large gear wheels (P. Sommer); (2) Damages by faulty heat treatment - Case studies: Glow testing at a heat exchanger and wheel breakage of a high-strength screw connection (A. Thomas); (3) Crack formation in pole end plates of high-performance generators of a pumped-storage power plant - Causes and possibilities of remedy (J. Kinder); (4) Grind burn inspection for damage prevention at wind turbine gearboxes - Use of different processes for the investigation of peripheral-zone properties of case-hardened components (T. Griggel); (5) Damage inspection in coal mines using products from the degradation process as an example - damage - inspection - solution: This is the working method of the certification body (C. Kleine-Hegermann); (6) Damages at the sealing rings - Causes of the failure at radial shaft rings (K. Marchetti); (7) Thermal analyses at faulty plastic components (O. Jacobs); (8) Application of the micro computer tomography at damages of fibre-reinforced materials (H. Dinnebier); (9) The significance of 'material defects' from the view of lay people, lawyers and engineers - 'Material defect' in the literature, set of rules and expert opinion (C. Klinger); (10) Material defects from a legal view (P. Henseler); (11) Significance of material defects from the view of an engineering insurer (C. Harden); (12) Wear analyses by means of RNT and non-destructive surface analytics (K. Poehlmann); (13) Damages by means of non-metallic inclusions using ICE 3 as an example - Significance and localisation of single non-metallic inclusions in large components (D. Bettge); (14) Cathodic corrosion protection of pipeline steels (H.-G. Schoeneich); (15) Non-destructive and destructive investigations when assessing damages of corrosion at a piping system (A. Zunkel); (16) Condition monitoring at metallic components in indoor swimming pools - Atmospheric loading and materials selection (M. Faller).

  10. Randschichthärtung von rostfreiem stahl durch Gasnitrierung und Gascarburierung bei niedringen Temperaturen

    DEFF Research Database (Denmark)

    Christiansen, Thomas; Somers, Marcel A.J.

    2005-01-01

    and carbon atoms are incorporated in the substrate under the development of expanded austenite. Crystallographic data, absorption isotherms, diffusion coefficients and the thermal stability were investigated for homogeneous foils of expanded austenite with different nitrogen contents. Hardness and residual...... treatments are enabled by a newly patented pretreatment, which replaces the passive chromium oxide layer by a very thin catalytic nickel layer. In the subsequent thermochemical treatment carbon and/or nitrogen containing species from the gas mixture dissociate at the Ni-surface. Simultaneously nitrogen...

  11. Zielgerichtete Chemotherapie solider Tumoren durch thermosensitive Liposomen in Kombination mit Doxorubicin, Gemcitabin und Mitomycin C

    OpenAIRE

    Limmer, Simone

    2014-01-01

    Auf DPPG2 basierende thermosensitive Liposomen (TSL) mit Hyperthermie (HT) induzierter zielgerichteter Wirkstofffreisetzung sind eine viel-versprechende Behandlungsstrategie in der Krebstherapie. TSL können als Wirkstoffträgersysteme die Zirkulationszeit und Anreicherung von Wirkstoffen im Zielgewebe erhöhen. Die vielfältigen Krebserkrankungen zeigen unterschiedliches Tumoransprechen auf die routinemäßig eingesetzten Zytostatika. Daher wäre es vorteilhaft, verschiedene Wirkstoffe in TSL e...

  12. Fulmar Litter EcoQO monitoring along Durch and North Sea coasts

    NARCIS (Netherlands)

    Franeker, van J.A.

    2011-01-01

    Wastes from ships are an important source of litter in the marine environment in the Southern North Sea and cause serious economic and ecological damage. Inadequacies in the ship to shore waste delivery procedures are considered a major factor in illegal discharges. The European Union addressed the

  13. Optimisation of industrial processes through modern maintenance management; Optimierung von Betriebsablaeufen durch modernes Instandhaltungsmanagement

    Energy Technology Data Exchange (ETDEWEB)

    Helmich, P.; Klocke, A.

    1997-12-31

    Maintenance is becoming an increasingly important issue for companies facing growing demands from the market with regard to quality, cost reduction, and security of supply. This gives rise to a multiplicity of tasks which have to be carried out in accordance with official licenses, legal regulations, guidelines, and ordinances. The present paper discusses requirements on maintenance planning and management tools (German: IPS). It deals with the technical features as well as with the cost/benefit aspects of the use of such tools. [Deutsch] Vor dem Hintergrund steigender Anforderungen des Marktes hinsichtlich Qualitaet, Kosteneinsparung und Lieferzuverlaessigkeit gewinnt die Instandhaltung in modernen Unternehmen zunehmend an Bedeutung. Die vielfaeltigen Aufgabenstellungen, die sich daraus ergeben, muessen unter Beruecksichtigung behoerdlicher Genehmigungen, einschlaegiger Gesetze, Richtlinien und Verordnungen durchgefuehrt werden. Im Beitrag werden die Anforderungen an Instandhaltungsplanungs und -steuerungswerkzeuge (IPS-Werkzeuge) diskutiert. Neben den technischen Merkmalen werden Kosten/Nutzen-Gesichtspunkte solcher Werkzeuge thematisiert. (orig./RHM)

  14. Teilflächenspezifisches Kartoffelpflanzen durch eine GPS-gestützte Pflanzgutablage

    Directory of Open Access Journals (Sweden)

    Yves Reckleben

    2014-08-01

    Full Text Available Precision management makes it possible to align cultivation intensity with demand and thus adjust expenses to the necessary level. The proportion of normal-sized, marketable commodity – i. e. size grade 40 to 60 mm – can thus be increased in potato growing too. Planting distances adapted to the soil properties seem to achieve this objective. Yields could be improved further, especially in intensive potato-growing regions where irrigation and fertilizing already contribute to a consistently high yield potential in the rather light soils. Different planting distances on individual soil plots were tested in the authors’ own trials. Planting distances of 31.50 cm in light soils, 24.50 cm in medium soils and 27.50 cm in heavy soils produced the best results. Both the overall yield and the proportion of marketable goods were improved.

  15. Rational use of energy by thermal insulation of residential buildings. Rationelle Energienutzung durch Waermeschutz von Wohngebaeuden

    Energy Technology Data Exchange (ETDEWEB)

    Feist, W. (Inst. Wohnen und Umwelt, Darmstadt (Germany))

    1992-01-01

    Processes in buildings need to be studied in detail to determine the technical potential of energy savings. Simulation models for thermal behaviour and simulation calculations prove that the technologies available today and the building input justifiable for the central-European climate will allow so-called passive-systems buildings to be built. Such a passive-systems building was built in Darmstadt Kranichstein. The heart of these passive-systems buildings is an excellent thermal insulation, its meticulous execution and the reduction of heat losses by ventilation. (BWI)

  16. Von und durch Neid erzählen. Rhetoriken des Neids in Konrads Engelhard

    Directory of Open Access Journals (Sweden)

    Eva Lieberich

    2016-12-01

    Full Text Available In academic debates of the 12th and 13th centuries, accusing one’s opponent of envy became a widespread feature. In the tradition of Roman literature, which connected virtus and invidia, the envy-reproach device was used to discredit critics as well as to strengthen one’s own position in intellectual discussions. This ‘rhetoric of envy’ was soon taken up in vernacular literature. Existing research has shown that envy was invoked in prologues and epilogues as a means for an author to secure a favorable reception of his work. This essay argues that envy was also used to direct the reader’s understanding of the histoire. By examining how and when the narrator talks about envy in Konrad’s Engelhard, I aim to show that envy reshapes the ethics of the narrative: In the trial scene, truthful accusations are transformed into envious speech acts. Envy shifts attention from the transgression of the protagonist to the moral failure of the antagonist. To conclude, I suggest the wider relevance of this special form of strategic narration by comparing Engelhard with other texts that involve the trial of a protagonist.

  17. Auswirkungen der Feldenkraismethode - Bewusstheit durch Bewegung - unter besonderer Berücksichtigung psychosomatischer Aspekte

    OpenAIRE

    Busch, Wolfgang

    2010-01-01

    Die vorliegende Arbeit beschäftigt sich mit den Auswirkungen von Feldenkrais-Bewegungsübungen auf ein Klientel, dass aus freien Stücken an Feldenkraiskursen eines niedergelassenen Feldenkraislehrers teilnimmt, wobei insbesondere gesundheitliche und psychosomatische Aspekte betrachtet werden. Feldenkrais formulierte die theoretischen Grundlagen der Methodik auf einem breiten wissenschaftlichen Fundament verschiedener Fachrichtungen, wie Medizin, Neurobiologie, Psychologie, Physik, Systemtheori...

  18. 222 Blasenhalssuspensionen nach Stamey und Burch: eine Erhebung der Langzeitergebnisse durch Fragebogen

    Directory of Open Access Journals (Sweden)

    Müller G

    2000-01-01

    Full Text Available Von 1984 bis 1996 haben wir 296 Patientinnen wegen einer Stressinkontinenz operiert. Während wir in den ersten Jahren die Nadelsuspensionen nach Stamey (n = 63 bevorzugten, wechselten wir später zur Kolposuspension nach Burch (n = 233. Unsere Untersuchung soll die Ergebnisse beider Operationsmethoden miteinander vergleichen. Die Frühkomplikationen stellten wir anhand der Krankenblätter fest. Eine Blasenentleerungsstörung trat bei 25,4 % (16/63 der nach Stamey Operierten auf, während dies nur bei 8,6 % (20/233 der nach Burch Operierten der Fall war. Nach der Stamey-Operation mußten bei 12,7 % (8/63 der Patientinnen ein oder beide Suspensionsfäden wieder entfernt werden. Dies war nach der Burch-Operation nicht erforderlich. Andere Komplikationen traten nur sporadisch auf. Zur Beurteilung des subjektiven Operationserfolges verschickten wir einen Fragebogen, der von 35 nach Stamey und von 187 nach Burch Operierten beantwortet wurde. Die mittlere Beobachtungszeit nach der Operation nach Stamey betrug 9,7 Jahre, nach der Operation nach Burch 4,5 Jahre. Der Zeitraum der absoluten Kontinenz nach der Operation wurde nach Angaben von 104 Patientinnen mit einem Follow-up von mindestens 6 Jahren festgestellt. 21,9 % (7/33 der nach Stamey und 8,4 % (6/71 der nach Burch Operierten wurden primär nicht völlig kontinent. Nach zwei Jahren waren nach beiden Operationen noch etwa 50 % absolut trocken und nach 7 Jahren etwa 30 %. Die subjektive Beurteilung des Operationsergebnisses war aber unterschiedlich: 56,2 % (105/187 der "Burch"-Patientinnen bezeichneten ihren Zustand als geheilt oder erheblich gebessert, 20,3 % (38/187 als mäßig gebessert und 23,5 % (44/187 als unverändert oder verschlechtert, während nur 42,9 % (15/35 der "Stamey"-Patientinnen einen Erfolg sahen, 22,9 % (8/35 einen mäßigen Erfolg und 34,2 % (12/35 einen Mißerfolg. Die Operation nach Burch hatte in unserem Krankengut weniger Frühkomplikationen zur Folge, und ihre Langzeitergebnisse wurden von den Patientinnen günstiger bewertet als das Verfahren nach Stamey, obwohl die Rezidivhäufigkeit nahezu gleich war.

  19. Pyritoxidationsprozesse im cretazischen Emschermergel infolge von aeroben Bedingungen durch den Emscherumbau

    Science.gov (United States)

    Droste, Björn; Wisotzky, Frank

    2015-09-01

    Due to the restoration of the Emscher river system, large amounts of the Emschermergel, a Cretaceous sediment containing significant amounts of carbonate and pyrite, will be released from its anoxic state. The oxidation of pyrite results in the release of protons, iron and sulfate. In order to evaluate the impact of the reaction products, extensive laboratory investigations were carried out. The methodology can detect pyrite content in the range of magnitude of the Rhenish lignite mining area (average 0.21 wt.% Pyritic-S). The formation of a naturally-occurring weathering horizon could also be detected. Under laboratory conditions, further oxidation of the weathered laboratory samples could be observed. Column experiments show significant release of sulfate at high concentrations. Hydrochemical modelling and acid-base accounting show neutralization of the free protons by the effective carbonate buffer in the sediment.

  20. Emanzipation durch Entwicklungspolitik? Einige Überlegungen zu Fragen globaler Ungleichheit

    Directory of Open Access Journals (Sweden)

    Daniel Bendix

    2015-09-01

    Full Text Available Seit es Entwicklungspolitik gibt, sieht sich diese mannigfaltiger Kritik ausgesetzt. Im Gegensatz zu anderen kritischen Sichtweisen geht es Post-Development-Ansätzen um die Infragestellung von Entwicklungspolitik an sich. Anstatt also vor allem Misserfolge und Mängel in den Blick zu nehmen, kritisieren Post-Development-Autor*innen Entwicklungspolitik auch in Fällen, in denen sie nach eigenen Maßstäben erfolgreich ist, und zwar u.a. mit dem Verweis auf die Reproduktion kolonialen Denkens, der Ausklammerung des problematischen Gesellschaftsmodells des Nordens, der Etablierung von Herrschaftsverhältnissen, der zerstörerischen Wirkung von Entwicklungshilfeprojekten und Modernisierung sowie der Reduktion eines guten Lebens auf sozioökonomische Indikatoren. Eine Politik, die globale sozioökonomische Ungleichheit als Problem sieht, ohne die Post-Development-Kritik zu ignorieren, muss sich der Frage stellen, wie eine solche auf der Grundlage der Reflexion und Umgehung der skizzierten problematischen Strukturen traditioneller Entwicklungszusammenarbeit aussehen kann. Dies führt dieser Beitrag aus.

  1. Förderung beruflicher Handlungskompetenz durch reflektiertes Erfahrungslernen mit digitalen Medien

    OpenAIRE

    Jan Hellriegel

    2017-01-01

    Eine gelingende Entwicklung beruflicher Handlungsfähigkeit in der dualen Berufsausbildung ist auf eine Kooperation von Lehrenden, Ausbildenden und Auszubildenden angewiesen. Häufig scheitert diese Zusammenarbeit jedoch an den spezifischen Lernkulturen und der räumlichen Trennung der verschiedenen Lernorte. Das BMBF-geförderte Forschungsprojekt KOLA: «Kompetenzorientiertes Lernen im Arbeitsprozess mit digitalen Medien» greift diese Problematik auf und verfolgt das Ziel, mithilfe digitaler Medi...

  2. Förderung beruflicher Handlungskompetenz durch reflektiertes Erfahrungslernen mit digitalen Medien

    Directory of Open Access Journals (Sweden)

    Jan Hellriegel

    2017-02-01

    Full Text Available Eine gelingende Entwicklung beruflicher Handlungsfähigkeit in der dualen Berufsausbildung ist auf eine Kooperation von Lehrenden, Ausbildenden und Auszubildenden angewiesen. Häufig scheitert diese Zusammenarbeit jedoch an den spezifischen Lernkulturen und der räumlichen Trennung der verschiedenen Lernorte. Das BMBF-geförderte Forschungsprojekt KOLA: «Kompetenzorientiertes Lernen im Arbeitsprozess mit digitalen Medien» greift diese Problematik auf und verfolgt das Ziel, mithilfe digitaler Medien eine Lernortkooperation zu befördern. Erprobt wird das entwickelte System, bestehend aus einer App und einer Browser-basierten Plattform, in saarländischen Ausbildungsstätten des Elektrohandwerks im Untersuchungszeitraum von Oktober 2014 bis September 2017. Im Rahmen dieses Projektes wird ein lerntheoretischer Ansatz verfolgt, der sich im Kontext von Konstruktivismus und Pragmatismus verorten lässt. Der vorliegende Beitrag fokussiert dabei das didaktische Konzept des Projektes, welches einerseits die didaktischen Grundannahmen und andererseits das hieraus abgeleitete Nutzungsszenario darstellt.

  3. Beeinflussung des Essentiellen Tremors und des Tremors beim Morbus Parkinson durch transkranielle Gleichstromstimulation

    OpenAIRE

    Hellriegel, Helge

    2010-01-01

    Ziel dieser Arbeit war es die Rolle des primärmotorischen Kortex bei der Entstehung und Weiterleitung des Essentiellen Tremors und des Tremors beim Morbus Parkinson zu untersuchen. Hierzu wurde eine transkranielle Gleichstromstimulation über dem primärmotorischen Kortex bei Patienten mit oben genannten Tremorsyndromen durchgeführt und die klinischen sowie elektrophysiologisch messbaren Änderungen des Tremors beurteilt.

  4. Abstrahierendes Lernen durch aktive Modellbildung: Evaluation eines Prozesses und einer Lernumgebung

    Directory of Open Access Journals (Sweden)

    Till Schümmer

    2015-11-01

    Full Text Available Experts and novices typically show different competencies in learning by abstraction. While novices typically consider the concrete example, experts often abstract from an example towards the underlying principles. This article presents a process and a computer-supported learning environment for learning through abstraction. An evaluation of the learning environment shows that pilot students were able to construct an abstract model from experience reports using the learning environment. The fewer common surface features the examples had, the easier was the abstraction. We also found that learners reflect on their learning process and adjusted the process to their specific learning preferences by following a modified process for learning through abstraction.

  5. Deblockierung durch Kooptation? Eine Fallstudie zur Aufnahme der Katholisch-Konservativen in die schweizerische Landesregierung 1891

    National Research Council Canada - National Science Library

    Christian Bolliger; Regula ZURCHER

    2004-01-01

    Kommt die Regierungsmehrheit in einer halbdirekten Demokratie gut organisierten Minderheiten nicht entgegen, riskiert sie, dass der Gesetzgebungsprozess mittels Volksrechten blockiert wird. Nach Neidhart (1970...

  6. In vitro -Untersuchungen zum Kaliumtransport durch das Epithel des Pansens beim Schaf

    OpenAIRE

    Oberhäuser, Simone

    2010-01-01

    The transport of potassium across the sheep rumen was studied with the USSING chamber technique by using 86Rb. a. In all experiments a net secretion of potassium was observed. There was a large variability of the unidirectional Rb fluxes. b. The manipulation of the Na transport and therefore the activity of the basolateral Na+-K+-ATPase by replacement of anions (Cl-, HCO3 -, SCFA) by gluconate or by a decrease of the incubation temperature did not influence transport of Rb significantl...

  7. Das Universum der Sterne. Himmelsbeobachtungen und Streifzüge durch die moderne Astronomie.

    Science.gov (United States)

    Rowan-Robinson, M.

    This book is a German translation, by M. Röser, of the English original published in 1990 (see 52.003.046). Contents: 1. Der Halleysche Komet. 2. Alpha Centauri - Der sonnennächste Stern. 3. Sirius und sein Begleiter, ein Weißer Zwerg. 4. Polaris - Der Polarstern. 5. Wega - Ein Planetensystem im Entstehen? 6. Mira - Der erste bekannte veränderliche Stern. 7. Der Ring-Nebel - "Todeskampf" eines sonnenähnlichen Sterns. 8. Der Orion-Nebel - Die Geburt neuer Sterne. 9. Delta Cephei - Die Meßlatte des Weltalls. 10. Algol - Der Teufelsstern. 11. Nova Aquilae - Der neue Stern von 1918. 12. Der Krebs-Nebel - Die Supernova des Jahres 1054. 13. Die Hyaden und Plejaden - Sternhaufen. 14. Die Milchstraße - Unsere Galaxis. 15. Die Magellanschen Wolken - Unsere Nachbarn im Weltall. 16. Der Andromeda-Nebel - Zwilling unserer Galaxis. 17. Messier 87 - Eine Radiogalaxie. 18. 3C 273 - Rätselhafter Quasar. 19. Messier 82 - Eine "Starburst"-Galaxie. 20. Der Virgo-Haufen - Ein reicher Galaxienhaufen. 21. Das Universum.

  8. Energy efficiency innovative processes during beer production; Rationelle Energienutzung durch innovative Verfahren bei der Bierwuerzebereitung

    Energy Technology Data Exchange (ETDEWEB)

    Antoni, D.; Stammel, V.; Meyer-Pitroff, R.

    2004-07-01

    The beverages industry is one of the most energy-intensive industries in the nutritional sector, and breweries are among the highest energy consumers. About 40 percent of the heat consumption of a brewery is used for beer heating. The contribution presents the example of thin film and vacuum evaporation in order to show how energy can be saved while maintaining or even improving the quality of the final product, saving resources and reducing CO2 emissions. (orig.)

  9. EUREGIO MRSA-net Twente/Münsterland : Search & Follow durch euregionale Netzwerkbildung

    NARCIS (Netherlands)

    Friedrich, A W

    2009-01-01

    Staphylococcus aureus causes the majority of healthcare-associated infections worldwide. Particularly critical are infections caused by methicillin-resistant Staphylococcus aureus (MRSA), for which there are few possibilities of antibiotic therapy. It is known that the occurrence of MRSA is directly

  10. Ländliche Entwicklung durch erneuerbare Energien - Das Beispiel Photovoltaik in Niederbayern

    Science.gov (United States)

    Zink, Roland

    2013-09-01

    Der Ausbau erneuerbarer Energien ist in Deutschland eine zentrale und politisch konsensfähige Forderung, um die Energieversorgung klimafreundlich und nachhaltig zu gestalten. Speziell ländliche Regionen rücken dabei in den Fokus, bieten sie doch aufgrund ihrer hohen Flächenverfügbarkeit große Potenziale für die flächenintensiven dezentralen Energieformen. Folglich erkennen Kommunen zunehmend das Entwicklungspotenzial dieser Technologien und sehen sie auch als Chance, neue wirtschaftliche Impulse vor Ort zu setzen. Der Beitrag skizziert die rasante Entwicklung der Photovoltaik im Regierungsbezirk Niederbayern und diskutiert anhand des Beispiels die Folgen für eine nachhaltige Regionalentwicklung.

  11. Decontamination of nuclear graphite by thermal processing; Dekontamination von Nukleargraphit durch thermische Behandlung

    Energy Technology Data Exchange (ETDEWEB)

    Florjan, Monika W.

    2010-04-15

    The main problem in view of the direct disposal of the nuclear graphite is its large volume. This waste contains long-lived and short-lived radionuclides which determine the waste strategy. The irradiated graphite possess high amount of the {sup 14}C isotope. The main object of the present work was the selective separation of {sup 14}C isotope from the isotope {sup 12}C by thermal treatment (pyrolysis, partial oxidation). A successful separation could reduce the radiotoxicity and offer a different disposal strategy. Three different graphite types were investigated. The samples originate from the reflector and from the flaking of spherical fuel elements of the high-temperature reactor (AVR) Juelich. The samples from the thermal column of the research reactor (Merlin, Juelich) were also investigated. The maximum tritium releases were obtained both in inert gas atmosphere (N{sub 2}) and under water vapour-oxidizing conditions at 1280 C and 900 C. Furthermore it could be shown that 28% of {sup 14}C could be released under inert gas conditions at a 1280 C. By additive of oxidizing agent such as water vapour and oxygen the {sup 14}C release could be increased. Under water vapour-oxidizing conditions at a temperature of 1280 C up to 93% of the {sup 14}C was separated from the graphite. The matrix corrosion of 5.4% was obtained. The selective separation of the {sup 14}C is possible, because a substantial part of the radiocarbon is bound near the grain boundary surfaces. (orig.)

  12. Vorsprung durch Technik: multi-display learning spaces and art-historical method

    OpenAIRE

    Bligh, Brett; Lorenz, Katharina

    2010-01-01

    The trajectory and heuristic success of Art History as a discipline has always been inseparably linked to the technical means of visualizing the material that is at its core. When in the late 19th century first analogous, then double-slide projection was introduced, associated methodological opportunities were identified and formalised through debate within the discipline. This led to a profound change in the discipline’s analytical rhetoric, installing vis-à-vis or comparative viewing as the...

  13. Verhandlung von FID-Lizenzen durch das Kompetenzzentrum für Lizenzierung – Statusbericht

    Directory of Open Access Journals (Sweden)

    Ursula Stanek

    2015-12-01

    Full Text Available Seit Januar 2014 steht das DFG-geförderte Kompetenzzentrum für Lizenzierung als Dienstleister für Fachinformationsdienste für die Wissenschaft (FID bereit. Nahezu alle Einrichtungen, die entweder bereits einen FID betreuen oder 2014 einen entsprechenden DFG-Antrag stellten, haben sich mit Verhandlungsaufträgen für eine Vielzahl von Produkten oder mit Interesse an der Nutzung der technischen Infrastruktur beim Kompetenzzentrum gemeldet. Es wird schwerpunktmäßig über den aktuellen Stand hinsichtlich der Verhandlung und des Abschlusses von FID-Lizenzen berichtet. Since January 2014 all Specialized Information Services for Research (FID can make use of the services of the Centre of Competence (KfL funded by the DFG. Almost all institutions which already provide an FID or intend to submit a proposal for an FID have contacted the KfL. Most institutions request negotiations for various electronic resources, some are also interested in using the technical infrastructure of the KfL. The article concentrates on the experience in negotiating FID-licenses.

  14. Geschlechterunterschiede beim kardialen Remodeling der extrazellulären Matrix durch Laufradtraining und Myokardinfarkt im Mausmodell

    OpenAIRE

    Ehrenberg, Nadine

    2010-01-01

    Cardiovascular diseases, especially the myocardial infarction, are the major cause of death in industrialized countries. Incidence and mortality show obvious gender-differences. Until menopause, women rarely have myocardial infarctions and mortality is also low. Postmeno-pausal however, the risk adapts quite fast and incidence and mortality rise. The reasons for the higher mortality in males are at least in the mouse model more fatal cardiac adaptations in extracellular matrix remodeling. Mal...

  15. Wie man Elementarteilchen entdeckt vom Zyklotron zum LHC : ein Streifzug durch die Welt der Teilchenbeschleuniger

    CERN Document Server

    Freytag, Carl

    2016-01-01

    Dieses Buch erklärt die physikalischen Grundlagen und die Technologien der Elementarteilchenforschung und beschreibt allgemeinverständlich Teilchenbeschleuniger und -detektoren sowie ihr Zusammenspiel. An einigen Meilensteinen der Forschung – von der Erzeugung von Transuranen über die Entdeckung exotischer Mesonen bis zum Higgs-Boson – zeigen die Autoren den Weg von der Theorie über das Experiment zum Forschungsergebnis auf. Gravitonen, Higgs-Teilchen, Neutrinos und Quarks – das Interesse an den kleinsten uns bekannten Teilchen ist seit Jahrzehnten ungebrochen und rückt damit auch die Laboratorien in den Blick, die an die Grenzen der Physik vorstoßen: Neben dem größten Experimentierfeld, das wir haben – dem Universum selbst - sind es die gigantischen Maschinen der Elementarteilchenphysik in Großforschungseinrichtungen wie dem CERN und dem DESY. Mit ihnen versuchen Forscher weltweit unter Einsatz extrem hoher Energien Zustände zu simulieren, wie sie zum Beginn unseres Universums kurz nach d...

  16. Operative Behandlung der Hemiplegia laryngis sinistra beim Pferd durch Transplantation des Nervus cervicalis 1

    OpenAIRE

    Molnar, Laslo

    2010-01-01

    The transplantation of the nervus cevicalis 1 as a surgical method of treating hemiplegia laryngis sinistra was first published by Fulton et al. (1991 and 1992). The surgical method described in this publication is based on the findings in those publications. This technique of transplanting the nervus cervicalis 1 as a donor nerve attempts to restore the function of the musculus cricoarytenoideus dorsalis under stress. In the course of this work preliminary anatomic studies of 32 horses’ head...

  17. Energy saving by smart metering with consumption feedback; Energieeinsparung durch Smart Metering mit Verbrauchs-Feedback

    Energy Technology Data Exchange (ETDEWEB)

    Hoffmann, Patrick [Institut fuer ZukunftsEnergieSysteme (IZES), Saarbruecken (Germany); Friedrich, Malte [Institut fuer Soziologische Meinungsforschung (IsoMe), Berlin (Germany); Kerber-Clasen, Stefan [Institut fuer Sozialforschung und Sozialwirtschaft e.V., Saarbruecken (Germany); Frey, Guenther

    2012-08-15

    While the German legislative, e.g. in the EnWG (Power Industry Act) assumes that electricity consumption metering will contribute to energy conservation in any case, many studies have arrived at a different contribution: Only a combination of consumption metering and direct feedback methods will result in significant energy savings. A recent research project (''Moderne Energiesparsysteme fuer Haushalte'') analysed an energy conservation system of this type. The findings will provide socio-economic insight into the background of successful energy conservation, and they will show how feedback methods can be optimised.

  18. Intensified fermentation of sewage sludge by ultrasonic disintegration; Intensivierung der Schlammfaulung durch Klaerschlammdesintegration mit Ultraschall

    Energy Technology Data Exchange (ETDEWEB)

    Nickel, K. [WAVES Wasser- u. Umwelttechnologien GmbH, Hamburg (Germany); Neis, U. [TU Hamburg-Harburg, Hamburg (Germany)

    2003-07-01

    Biodegradation of municipal sewage sludge is often limited due to low biological availability of sludge particles. Enzymatic hydrolysis is slow and therefore anaerobic fermentation and biogas yield is not efficient. Hydrolysis can be accelerated by sludge desintegration and cell disruption with ultrasound in the frequency range above 20 kHz through cavitation effects. The article describes potential and experiences with ultrasound pretreatment of sewage sludge. (uke)

  19. Energy obtained from the sea - controlled energy conversion cell. Energiegewinnung durch Seegang - gesteuerte Energieumwandlungszelle

    Energy Technology Data Exchange (ETDEWEB)

    Gierl, A.

    1982-02-25

    The use of energy from the sea is obtained by pumping water to a higher level, from which the potential energy can be converted into electricity. The energy absorbed is given up by a tube, which represents a piston pump, via a lifting body which is forced into vertical movement between guides. In order to minimise the friction losses, the height of the lifting body must be small compared to double the wave height. Also, the force of piston resistance must be made less than thhe weight of the lifting body. Thus the height of lift becomes a maximum, and the lifting body is only lowered when it is free of hydrodynamic upthrust forces.

  20. Lässt sich Untreue durch Geschlecht, Einstellung oder Persönlichkeit vorhersagen?

    OpenAIRE

    Helms, Lillan; Bierhoff, Hans-Werner

    2001-01-01

    "Diese Untersuchung befasst sich mit der Frage, ob sich Männer und Frauen hinsichtlich der Häufigkeit und der Art der Untreue unterscheiden und ob Liebesstile, Bindungsstile, Sexuelle Einstellungen und Geschlechtsrollenorientierung einen Einfluss auf das berichtete Untreueverhalten haben. Befragt wurden 96 Personen zwischen 19 und 35 Jahren, die zur Zeit der Untersuchung in einer Beziehung lebten. Die Ergebnisse zeigten, dass Männer und Frauen gleich häufig über Untreue berichteten und dass s...