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Sample records for duodenum

  1. [Vascular compression of the duodenum].

    Science.gov (United States)

    Acosta, B; Guachalla, G; Martínez, C; Felce, S; Ledezma, G

    1991-01-01

    The acute vascular compression of the duodenum is a well-recognized clinical entity, characterized by recurrent vomiting, abdominal distention, weight loss, post prandial distress. The cause of compression is considered to be effect produced as a result of the angle formed by the superior mesenteric vessels and sometimes by one of its first two branches, and vertebrae and paravertebral muscles, when the angle between superior mesenteric vessels and the aorta it's lower than 18 degrees we can saw this syndrome. The duodenojejunostomy is the best treatment, as well as in our patient.

  2. Pseudomelanosis of stomach, duodenum, and jejunum.

    Science.gov (United States)

    Rustagi, Tarun; Mansoor, Muhammad S; Gibson, Joanna A; Kapadia, Cyrus R

    2015-02-01

    Pseudomelanosis is a rare finding during upper gastrointestinal endoscopy, and is most commonly seen in the duodenum. Involvement of other organs in the upper gastrointestinal tract is extremely rare, with only 1 reported case involving the stomach, duodenum, and jejunum. We present a case of a 60-year-old woman with mild anemia and hematemesis, who was found to have characteristic speckled pattern of gray-black pigmentation on endoscopic examination. To the best of our knowledge, this is the second reported case of pseudomelanosis involving the stomach, duodenum, and jejunum.

  3. Gastrointestinal stromal tumor (gist) of the duodenum.

    Science.gov (United States)

    Ghazanfar, Shahriyar; Sial, Khadim S; Quraishy, M S

    2007-06-01

    This is a report of a rare gastrointestinal stromal tumor of the duodenum in a 75 years old man who presented with recurrent episodes of intestinal obstruction and melena. The patient underwent successful Whipple's procedure.

  4. Vagal innervation of the rat duodenum.

    Science.gov (United States)

    Zhang, X; Renehan, W E; Fogel, R

    2000-02-14

    Electrophysiologic and anterograde tract tracing studies have demonstrated that the vagus nerve innervates the duodenum. These studies, however, have provided little information regarding the finer anatomic topography within the vagal complex. In this study, the retrograde neuronal tracers WGA-HRP or DiI, applied to the duodenum, were used to characterize the vagal afferent and efferent innervation of this portion of the gastrointestinal tract. This approach labeled a substantial number of motor neurons in both the medial and lateral columns of the dorsal motor nucleus of the vagus (DMNV). Vagal motor neurons innervating the duodenum were seen across the medial-lateral extent of the DMNV and between 600 microm rostral to obex and 1600 microm caudal to obex. The three branches of the vagus nerve contained efferent fibers to the duodenum. The gastric branch of the vagus nerve was the pathway that connected the majority of DMNV neurons with the duodenum. These neurons were located in the medial and middle thirds of the DMNV. The celiac branch to the duodenum was composed of axons from the majority of lateral column neurons but also contained axons from neurons in the medial column. The hepatic branch of the vagus nerve contained only a small number of cell axons. Some neurons were located medially whereas others were in the lateral third of the duodenum. Although central terminations of vagal primary afferents from the duodenum were not found in previous tract tracing studies, we observed a large number of terminals in the subpostremal/commissural region of the nucleus of the solitary tract. Similar to the motor fibers, most afferent fibers from the duodenum were located in the gastric branch of the vagus nerve, although the hepatic and celiac branches also contained afferent neurons. These results demonstrate that the vagal innervation of the duodenum is unique, being an amalgam of what would be expected following labeling of more proximal and distal portions of the

  5. Neural connections between antrum and duodenum

    DEFF Research Database (Denmark)

    Kraglund, K; Schrøder, H D; Stødkilde-Jørgensen, H

    1983-01-01

    Postprandial coordination of antroduodenal motility partly takes place via intrinsic mural pathways. The nature and origin of these nerve fibers have not yet been clarified. In this investigation using fluorochromic substances injected into the antrum and duodenum it was demonstrated that common...

  6. Dieulafoy's lesion of duodenum: a case report

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    Wagholikar Gajanan D

    2003-01-01

    Full Text Available Abstract Background Dieulafoy's lesion is an uncommon but important cause of recurrent upper gastrointestinal bleeding. Extragastric location of Dieulafoy's lesion is rare. We report two cases of Dieulafoy's lesion of the duodenum and discuss the management of this extremely uncommon entity. Case presentation Two cases of massive upper gastro-intestinal bleeding in young adults due to Dieulafoy's lesion of the duodenum are reported. Endoscopic diagnosis was possible in both cases. Hemostasis was achieved successfully by endoscopic adrenaline injection. The endoscopic appearance, pitfalls in the diagnosis and management of this rare lesion are discussed. Conclusions Endoscopic diagnosis of extragastric Dieulafoy's lesion can be difficult because of the small size and obscure location of the lesion. Increased awareness and careful and early endoscopic evaluation following the bleeding episode are the key to accurate diagnosis. Adrenaline injection is one of the important endoscopic modalities for control of bleeding.

  7. Nonceliac Diaphragm Disease of the Duodenum

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    Hugh J Freeman

    2000-01-01

    Full Text Available A 58-year-old, Indo-Canadian man presented with upper abdominal pain and the clinical features of a partial, gastric outlet obstruction. Subsequent studies, including endoscopic examination, revealed diaphragm-like strictures in the descending duodenum. Other reported causes such as celiac disease and drug-induced small bowel diaphragms were excluded. Possibly, the changes seen in this patient were related to ethnic food-induced, mucosal injury to the upper gastrointestinal tract. Further studies are needed to evaluate potential toxicity or protective effects of different ethnic diets and their relationship with the development of different intestinal diseases.

  8. Bouveret syndrome: gallstone ileus of the duodenum.

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    Englert, Zachary P; Love, Katie; Marilley, Mark D; Bower, Curtis E

    2012-10-01

    This is a case of a 59-year-old woman with Bouveret syndrome. An initial endoscopic approach to management is described. Gallstone ileus occurs when a gallstone passes from a cholecystoduodenal fistula or a choledochoduodenal fistula into the gastrointestinal tract and causes obstruction, usually at the ileocecal valve. Bouveret syndrome is a variant of gallstone ileus where the gallstone lodges in the duodenum or pylorus causing a gastric outlet obstruction. The endoscopic and surgical management of this process are important to keep in mind and may be evolving as endoscopic therapies improve.

  9. Cystic Brunner's gland hamartoma in the duodenum: A case report

    Institute of Scientific and Technical Information of China (English)

    Beom Jin Park; Min Ju Kim; Jeong Hyeon Lee; Sung-Soo Park; Deuk Jae Sung; Sung Bum Cho

    2009-01-01

    Cystic Brunner's gland hamartoma in the duodenum is exceedingly rare, although microscopic examination may sometimes reveal a Brunner's gland hamartoma containing dilated ducts in the duodenum. We present a case of large cystic Brunner's gland hamartoma in the duodenum with a long stalk, which is described in light of multidetector-row computed tomography, magnetic resonance imaging, and a modified small bowel series, together with pathologic correlation and differential diagnosis.

  10. Malignant phyllode tumor metastatic to the duodenum

    Institute of Scientific and Technical Information of China (English)

    Oktar Asoglu; Hasan Karanlik; Umut Barbaros; Hakan Yanar; Yersu Kapran; Mustafa Kecer; Mesut Parlak

    2006-01-01

    Phyllode tumor (PT) is extremely rare tumor of the breast. Distant metastasis occurs in 10-20% of patients with malignant phyllode tumor. The most common sites of metastases are the lungs and bones. Although theoretically any organ may have metastasis, an isolated duodenum metastasis has not been documented as yet in the English-language literature. We report herein a case with a isolated duodenal metastasis from PT of breast in a 31 year-old-woman who underwent right mastectomy 4 years before because of the recurrent malignant PT. She presented to our hospital with massive upper gastrointestinal bleeding. Clinical evaluation revealed a huge mass originated from duodenum. Urgent laparotomy and pancreaticoduodenectomy were carried out in order to remove the bleeding duodenal mass.The pathologic examination of the resected specimen showed a malignant spindle cell tumor consistent with metastatic malignant PT. Our case of gastrointestinal bleeding due to an isolated duodenal metastasis as a result of hematogenous spread from malignant phyllode tumor of breast is unique in the English literature and pancreaticoduodenectomy is a curative treatment for patients with isolated duodenal involvement.

  11. Inverted cystic tubulovillous adenoma involving Brunner's glands of duodenum

    Institute of Scientific and Technical Information of China (English)

    Ji Hoon Kim; Jong-Jae Park; Jung Woo Choi; Yeon Seok Seo; Beom Jae Lee; Jong Fun Yeon; Jae Seon Kim; Kwan Soo Byun; Young-Tae Bak; Insun Kim

    2007-01-01

    Benign neoplasia of the duodenum are very rare.Moreover, duodenal tubulovillous adenomas are more uncommon lesions. The microscopic structure of tubulovillous adenoma has frond-like projection of mucosa with branching papillary structure and generally upward growth into the lumen. We describe a 72-year-old man who showed aduodenal tubulovillous adenoma with unusual inverted cystic growth pattern.Interestingly, this tubulovillous adenomatous lesion was interrupted by gastric metaplasia in the deep portion of the cyst and was closely surrounded by Brunner's glands. Although histogenesis of gastric metaplasia of duodenum is not fully understood, Brunner's glands has been suggested as a precursor for gastric metaplasia.Therefore, these findings argued that this adenoma arises from Brunner's glands through gastric metaplasia.This is the first case of inverted cystic tubulovillous adenoma involving Brunner's glands of duodenum with gastric metaplasia.

  12. Emerging role of narrow band imaging in duodenum

    Institute of Scientific and Technical Information of China (English)

    Amit; Kumar; Dutta; Ashok; Chacko

    2015-01-01

    Endoscopy using magnification narrow band imaging(m NBI) allows detailed assessment of mucosal surface and vascular pattern. This may help in better identification and prediction of the nature of the lesion. The role of this technology in duodenum is still evolving. Studies have shown that m NBI has high accuracy in predicting villous atrophy in the duodenum. Limited data suggests that this technique can provide additional information on duodenal polyps,nodules and ampullary tumour which can help guide their management. In this paper we describe the technique for duodenal assessment using NBI and review the existing literature evaluating its role in diagnosis of various duodenal pathologies.

  13. Carcinoma of the duodenum after trauma, radiotherapy and chemotherapy.

    Science.gov (United States)

    Bayens, Y C; Wiggers, T; Meerwaldt, J H; Vroom, T M; Van Geel, A N

    1991-10-01

    The case history is reported of a patient with a carcinoma of the duodenum 30 years after blunt abdominal trauma at the site of the 'scar' in the duodenum. Thirteen years after the trauma the patient was treated with chemotherapy and abdominal irradiation for a relapse of Hodgkin's disease. At follow-up, 25 months after the operation, he had no local recurrence of Hodgkin's disease or duodenal cancer. The possible relation between the cancer and the abdominal trauma, chemotherapy and abdominal irradiation is discussed.

  14. Obstructing Gangliocytic Paraganglioma in the Third Portion of the Duodenum

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    Carlos M. Nuño-Guzmán

    2012-07-01

    Full Text Available Gangliocytic paragangliomas are infrequent tumors almost exclusively found in the second portion of the duodenum. An unusual case of a gangliocytic paraganglioma in the third portion of the duodenum with obstructive symptoms is herein reported. A 16-year-old male patient presented with epigastric pain, postprandial plenitude and reflux. A barium swallow failed to demonstrate abnormalities. Endoscopy showed a pedunculated submucosal tumor, originating at the third duodenal portion and causing partial obstruction. Biopsy was not performed due to the risk of bleeding. CT scan demonstrated a polypoid lesion. Through a transmesocolic approach and an anterior duodenotomy, resection of the tumor was performed. No lymph node or other organ affection was found. Histologic examination revealed a gangliocytic paraganglioma. Immunohistochemical examination was performed. Gangliocytic paragangliomas originating in the third or fourth portion of the duodenum, as in the present case, are extremely rare. Characteristic histologic features including epithelioid cells, spindle-shaped cells and ganglion-like cells were met. The majority of cases manifest with a similar benign behavior. Local resection of the tumor is recommended for these cases. An infrequent case of a gangliocytic paraganglioma located in the third portion of the duodenum, with a less common clinical presentation, is herein reported.

  15. Perforation of the duodenum by an ingested toothbrush

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    We report a rare case of duodenal perforation caused by an ingested 12-cm long toothbrush handle. A 22-year-old female presented with intermittent epigas- tric pain for 6 d after swallowing a broken toothbrush. The swallowed toothbrush could not be removed from the second portion of the duodenum by endoscopy. Laparotomy revealed a perforation in the anterior wall of the duodenal bulb. The toothbrush was removed via the perforation which was debrided and closed. There were no postoperative complications.

  16. Perforation of the duodenum by an ingested toothbrush

    OpenAIRE

    Chao, Hsiao-Hsiang; Chao, Tzu-Chieh

    2008-01-01

    We report a rare case of duodenal perforation caused by an ingested 12-cm long toothbrush handle. A 22-year-old female presented with intermittent epigastric pain for 6 d after swallowing a broken toothbrush. The swallowed toothbrush could not be removed from the second portion of the duodenum by endoscopy. Laparotomy revealed a perforation in the anterior wall of the duodenal bulb. The toothbrush was removed via the perforation which was debrided and closed. There were no postoperative compl...

  17. STRUKTUR HISTOLOGI DUODENUM, JEJENUM, DAN ILEUM SAPI BALI

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    Ni Nyoman Werdi Susari

    2009-03-01

    Full Text Available A study to find out the histological structures of small intestine (ie. duodenum, jejunum, and ileum of bali cattle have been carried out. A total of 20 small intestines were collected from the abattoir at Pesanggaran, Denpasar Bali. Histological examinations were performed on Haematoxyline Eosin stained small intestines slides. The duodenum, jejunum and ileum of the small intestines have four mucosa membranes: i mucosa membrane, ii sub mucosa membrane, iii muscular membrane and, iv serous membrane. The average thickness of mucosa membrane of duodenum, jejunum and ileum was 36,3±13,5 mm; 29,9±3,1 mm; and 38,8±11,9 mm, respectively. Each part has villi with an average length of 27,5±8,3 mm; 20,1±3,7 mm; and 18,5±4,3 mm, respectively. This mucosa membranes consist of single columnar epithelium, muscularis mucosa, and lamina propria. The average thickness of sub mucosa membrane was 47,3±15,3 mm; 10,4±2,6 mm; and 16,9±5,6 mm, respectively. Sub mucosa membrane consisted of connective tissue. The average thickness of muscular membrane was 46,9±8,8 mm; 28,1±5,1 mm; and 62,4±11,3 mm, respectively, which consisted of circular and longitudinal smooth muscle. The average thickness of serous membrane was 19,9±3,1 mm; 11,9 ±3,0 mm; and 12,1±3,6 mm, respectively, which consisted of mainly connective tissue. Goblet cells were seen through all the epithel of small intestines with the highest number seen in the ileum. Specific structure of duodenum, jejunum, and ileum which is Brunner’s glands in the sub mucosa membranes of duodenum, circular plicae in the jejunum and mesenteric gland in the ileum were also observed.

  18. Strongyloidiasis of duodenum clinically masquerading as gastric malignancy

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    V Srinivasa Murthy

    2013-01-01

    Full Text Available A 58-year-old male patient presented with pain abdomen since 1 month and bilious vomiting since 1 week. After admission to our hospital, in view of his age and clinical presentation, upper gastrointestinal endoscopy was done and biopsy sent for histopathological examination. A diagnosis of Strongyloidiasis of duodenum was made. Herein, we report such an unusual case, where the diagnosis was first made by duodenal biopsy, rather than simple stool examination. Since the case clinically masqueraded as gastric malignancy, the importance of routine simple stool examination and role of pathologist in identifying the parasite is hereby highlighted.

  19. Roll seat belt induced injury of the duodenum.

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    Bergqvist, D; Hedelin, H

    1976-05-01

    A case of duodenal rupture with a roll three-point seatbelt is described. It is apparently the seventh reported case of duodenal rupture in safety belt users. A female driver fell asleep, and her car went off the road, rolling forward in a ditch, slowing slightly, and then came to a sudden stop. The rupture was unusual: on the first part of duodenum, intraperitoneal, and longitudinal. The rupture mechanism is discussed, and the deficiencies of the roll seatbelt pointed out in accidents like the one described.

  20. Gallstone ileus of duodenum with huge duodenal stone

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    Mohammad Ehsan

    2006-12-01

    Full Text Available Gastrointestinal obstruction by a gallstone is an uncommon but important complication of biliary stone disease which mostly affects the elderly. The classic triad of radiological features includes pneumobilia, ectopic gallstone and evidence of intestinal obstruction. Terminal ileum is the most common site of obstruction, followed by jejunum and gastric outlet. We present a case of duodenal gallstone ileus of a large, fluid-density mixed biliary stone with a peripheral rim of hyperdensity (very fine calcification in CT scan. KEY WORDS: Gallstone ileus, duodenum, intestinal obstruction.

  1. Large gangliocytic paraganglioma of the duodenum:A rare entity

    Institute of Scientific and Technical Information of China (English)

    Alejandra; Gordillo; Hernández; Eduardo; Dominguez-Adame; Lanuza; Auxiliadora; Cano; Matias; Rosario; Perez; Huertas; Katherine; Maria; Gallardo; Rodriguez; Purificacion; Gallinato; Perez; Fernando; Oliva; Mompean

    2015-01-01

    Gangliocytic paragangliomas are rare tumors that almost exclusively occur within the second portion of the duodenum. Although these tumors generally have a benign clinical course, they have the potential to recur or metastasize to regional lymph nodes. The case report presented here describes a 57-year-old female patient with melena, progressive asthenia, anemia, and a mass in the second-third portion of the duodenum that was treated by local excision. The patient was diagnosed with a friable bleeding tumor. The histologic analysis showed that the tumor was a 4 cm gangliocytic paraganglioma without a malignant cell pattern. In the absence of local invasion or distant metastasis, endoscopic resection represents a feasible, curative therapy. Although endoscopic polypectomy is currently considered the treatment of choice, it is not recommended if the size of the tumor is > 3 cm and/or there is active or recent bleeding. Patients diagnosed with a gangliocytic paraganglioma should be closely followed-up for possible local recurrence.

  2. CYSTIC DEGENERATION OF THE DUODENUM ASSOCIATED WITH CHRONIC PANCREATITIS

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    A. G. Kriger

    2016-01-01

    Full Text Available Inflammatory cystic lesion of the descending part of the duodenum, mainly in the field of small duodenal papilla in chronic pancreatitis (CP is described as “duodenal dystrophy” (DD. The pathogenesis of this condition has not been studied and treatment strategy is not defined.Purpose. Investigation of cystic inflammatory transformation of duodenal wall pathogenesis in patients with CP, described as a DD, and evaluate the clinical ef ficiency of surgical treatment.Material and methods. Eighty two patients with DD were retrospectively included over 12 years. The diagnosis of DD was established by transabdominal ultrasound, CT, MRI and endosonography. Initially, all patients were treated conservatively. 74 patients required surgical treatment subsequently after conservative treatment with a median duration of 2 years. 34 patients underwent pancreaticoduodenectomy (PD, 21 patients underwent duodenum resection, 15 – duodenum preserving pancreatic head resection of (DPPHR. 4 patients underwent palliative operations. Diagnosis of CP and DD was verified by histological study of surgical specimens. Not operated patients (8 are under observation. Long-term results of surgical treatment were evaluated in 47 patients with a median follow-up was 49.9 months.Results. Histological examination resulted that in 69.9% of DD was related with groove pancreatitis, with ectopic pancreatic tissue – in 30.1%. DD was associated with CP in 92.6% of cases. Clinical presentation of DD was not related with etiology and showed typical symptoms of CP: abdominal pain occurred in 98.8% of patients, body weight loss – 61.7%, duodenal obstruction – 35.8%, biliary hypertension – 34.1%. The overall morbidity was 35.1%. Overall postoperative mortality was 1.37% (1 patient. 66% of patients had no clinical symptoms postoperatively, a significantimprovement – 32%, no effect – 2%.Conclusion. The most cases of DD is related with groove pancreatitis, less

  3. [Clinical-epidemiological aspects of gastric and duodenum ulcer].

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    Larichev, A B; Maĭorov, M I; Favstov, S V; Shalop'ev, A G

    2014-01-01

    An analysis of reporting and statistical data showed the considerable changes in clinical-epidemiological indices of gastric and duodenum ulcer at the period from 1998 to 2012. The prevalence of the disease and the number of primary patients decreased in 2-3 times. The reduction of the rate of perforations and ulcerous bleeding had been observed. However, the authors noted, that a tendency of frequency of occurrence increased and efficacy indices reduced in the last years. The rate of postoperative lethality raised in the cases of perforated ulcer. On this basis, the authors recommend to reconsider the existing opinion about further extension of out-patient treatment of patients with given pathology.

  4. Biological clocks in the duodenum and the diurnal regulation of duodenal and plasma serotonin.

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    Elizabeth Ebert-Zavos

    Full Text Available Serotonin in blood plasma is primarily synthesized in the duodenum, as brain derived serotonin does not cross the blood-brain barrier. Because serotonin in the brain and retina is synthesized under the control of a circadian clock, we sought to determine if a circadian clock in the duodenum regulates serotonin synthesis and release in blood. We examined gene expression in the duodenum of chickens at different times of the day and found that the duodenum rhythmically expresses molecular circadian clock genes and genes controlling serotonin biosynthesis, specifically tryptophan hydroxylase, in a light dark cycle (LD. Analysis of the duodenum and blood plasma showed that the amount of serotonin in the duodenum varies across the day and that serotonin profiles in blood plasma are also rhythmic in LD, but were not rhythmic in constant darkness. Because serotonin in the gut affects duodenal nutrient absorption and gut motility, the control of serotonin production in the duodenum by LD cycles could provide an additional mechanism by which the external environment controls nutrient uptake and digestive function. The diurnal regulation of plasma serotonin may also serve as an additional biochemical signal in the blood encoding time and could be used by target tissues to indicate the status of nutrient absorption.

  5. After 3 years of starvation: duodenum swallowed remaining stomach.

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    Hillenbrand, Andreas; Waidner, Uta; Henne-Bruns, Doris; Maria Wolf, Anna; Buttenschoen, Klaus

    2009-05-01

    A 42-year-old morbidly obese patient (BMI 44.1 kg/m(2)) was admitted to our emergency room with upper abdominal pain, nausea, and cholestasis. Nine years ago, a vertical banded gastroplasty had been performed (former BMI 53.5 kg/m(2)) with a subsequent weight loss to BMI 33.0 kg/m(2). After regaining weight up to a BMI of 47.6 kg/m(2), 5 years ago a conversion to a gastric bypass was realized. A computed tomography of the abdomen showed an invagination of the remaining stomach into the duodenum causing obstruction of the orifice of common bile duct. The patient underwent an open desinvagination of the intussusception and resection of the remaining stomach. Gastroduodenal intussusception is rare and mostly secondary to gastric lipoma. To prevent this rare but serious complication, the remaining stomach could be fixed at the crura of the diaphragm, tagged to the anterior abdominal wall by temporary gastrostomy tube, or resected.

  6. Retroperitoneal perforation of the duodenum from biliary stent erosion.

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    Miller, George; Yim, Duke; Macari, Michael; Harris, Marsha; Shamamian, Peter

    2005-01-01

    Endoscopically placed biliary stents have supplanted surgical decompression as the preferred treatment option for patients with obstructive jaundice from advanced pancreatic cancer. An unusual complication of indewelling biliary stents is duodenal perforation into the retroperitoneum. We describe the case of a patient with end-stage pancreatic cancer who presented with an acute abdomen from erosion of a previously placed bile duct stent through the wall of the second portion of the duodenum. Although our patient presented with advanced symptoms, clinical presentations can vary from mild abdominal discomfort and general malaise to overt septic shock. Definitive diagnosis is best made with computed tomography (CT) imaging, which can detect traces of retroperitoneal air and fluid. Treatment options vary from nonoperative management with antibiotics, bowel rest, and parenteral alimentation in the most stable patients to definitive surgery with complete diversion of gastric contents and biliary flow from the affected area in patients with clinical symptoms or radiologic evidence suggesting extensive contamination. Complications of management can include duodenal fistulization, residual retroperitoneal or intrabdominal abscess, and ongoing sepsis. This report highlights the salient issues in the presentation, diagnosis, and modern management of patients with this rare complication of indwelling biliary stents.

  7. Angiodysplasia in gaint diverticulum of transverse duodenum causing massive gastrointestinal bleeding: a case report

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    Choi, Pil Yeob; Lee, Sang Wook; Kwon, Jae Soo; Sung, Young Soon; Rho, Myoung Ho; Hwon, Oh Joon [Sungkyunkwan Univ. College of Medicine, Seoul (Korea, Republic of)

    1998-12-01

    The incidence of duodenal diverticulum found incidentally during upper gastrointestinal roentgenographic examination varies between 2% and 5%. The majority of cases occur along the medial aspect of the second portion of the duodenum, within 2.5 cm of the ampulla of Vater. The majority of duodenal diverticual are asymptomatic, but in some cases, complications such as diverticulitis, hemorrhage, perforation, and fistula formation occur in the third and fourth portions of the duodenum. We describe a case of giant diverticulum of the transverse duodenum, revealed by UGI and angiography, massive gastrointestinal bleeding in a 80-year-old patient.=20.

  8. Brunner's gland adenoma of duodenum:A case report and literature review

    Institute of Scientific and Technical Information of China (English)

    Yu-Ping Gao; Jian-Shan Zhu; Wen-Jun Zheng

    2004-01-01

    AIM: To analyze the clinicopathological features of Brunner's gland adenoma of the duodenum.METHODS: A rare case of Brunner's gland adenoma of the duodenum was described and related literature was reviewed.RESULTS: Brunner's gland adenoma of the duodenum appeared to be nodular hyperplasia of the normal Brunner's gland with an unusual admixture of normal tissues, including ducts, adipose tissue and lymphoid tissue. We suggested that it might be designated as a duodenal hamartoma rather than a true neoplasm.CONCLUSION: The most common location of the lesion is the posterior wall of the duodenum near the junction of its first and second portions. It can result in gastrointestinal hemorrhage and duodenal obstruction. Endoscopic polypectomy is a worthy treatment for benign Brunner's gland adenomas,as malignant changes in these tumors have never been proven.

  9. Serotonin- and Somatostatin-Positive Goblet Cell Carcinoid of the Duodenum

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    Ohara,Ichiyou

    2012-08-01

    Full Text Available In the duodenum, mixed exocrine-endocrine tumors exhibiting both neuroendocrine and glandular differentiations [cf. appendiceal goblet cell carcinoids (GCCs] are rare. We present a Japanese case with a duodenal GCC that was found during pathologic examination of a gastrectomy specimen removed for gastric mucosal cancer. The tumor was widely distributed within both the first portion of the duodenum and the gastric antrum, although mucosal involvement was observed only in the duodenum. The tumor cells formed solid nests, trabeculae, or tubules, and some displayed a goblet cell appearance. They were immunoreactive against antibodies for both serotonin and somatostatin, and showed an argentaffin reaction (similar to a “midgut” enterochromaffin cell carcinoid. Ultra-structurally, the tumor cells had an amphicrine nature. Physicians encounter GCC in the duodenum only rarely, and its discovery may be incidental. Its diagnosis will be challenging and will require careful clinical and pathologic examinations.

  10. Clips migration to duodenum as a rare complication of laparoscopic cholecystectomy

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    Muammer Bilici

    2016-03-01

    Full Text Available Endoclip migration into the duodenum is an extremely rare complication of laparoscopic cholecystectomy. The patients usually present with bleeding ulcer. Here we report a 65-year-old female patient with a complaint of abdominal pain and dyspepsia due to clip migration into the duodenum after laparoscopic cholecystectomy secondary to symptomatic cholelithiasis 15 months previously. Ultrasonography and liver function tests were normal. Endoscopy showed metal clips in the second part of duodenum. The clips were removed endoscopically. No active bleeding was noted. In this case report, we present diagnosis and management of clips migration into wall of duodenum as a complication of laparoscopic cholecystectomy. [Cukurova Med J 2016; 41(0.100: 71-74

  11. Intra Luminal Metastasis to Duodenum: A Histological Surprise

    Science.gov (United States)

    Aroul, Tirou; Kuppusamy, Sasikumar; Gunashekaran, Vijay

    2016-01-01

    Carcinoma cervix is one of the most common malignancies seen in women worldwide and more so in the Indian subcontinent. Carcinoma cervix is known for its orderly lymphatic spread. Skip metastasis or intraluminal metastasis is not a frequent occurrence. A 50-year-old diabetic and hypertensive female patient who was diagnosed to have carcinoma cervix (stage II A 2) Grade II to III and post radiotherapy (3 years back) presented to the surgical outpatient with dyspeptic symptoms and vague abdominal pain. On examination she was found to have a 1x1cm hard, fixed, left supraclavicular node and a palpable liver. Ultrasound abdomen revealed multiple retro peritoneal lymphadenopathy and Contrast Enhanced Computerised Tomography (CECT) abdomen revealed secondary deposits in the pancreatico-duodenal groove infiltrating the duodenal C-loop and pancreatic head, with multiple retroperitoneal nodes with necrosis. Fine needle Aspiration Cytology (FNAC) from left supraclavicular node revealed metastatic squamous cell carcinoma. Oesophagogastroduodenoscopy showed 2 sessile lesions in the anterior wall of duodenum with mucosa stretched and central umbilication with almost complete luminal obstruction. Biopsy from the duodenal lesion also turned out positive for metastatic squamous cell carcinoma. Gynaecological examination was negative for any local recurrence. Patient was managed symptomatically for dyspepsia and is currently undergoing chemotherapy. She is at present on regular follow-up and is asymptomatic for the bowel metastasis. Given the frequency of cancer cervix and the rarity of intra luminal metastasis, this case report serves to reiterate the fact that the abdomen is truly a pandora’s box. PMID:27790514

  12. Andrological aspects of the perforative ulcer of the duodenum

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    M. A. Nurtdinov

    2015-01-01

    Full Text Available Authors on a scale of vector definition of the sexual constitution investigated 115 patients with a perforative ulcer of a duodenum, in three age groups: the 1st from 18 to 28 years, the 2nd from 29 to 56 years and the 3 rd is more senior than 57 years. In this scale the following criteria are defined: the I vector – awakenings a libido; II – age of the first ejaculation; III – a trochanteric index (growth relation to foot length; IV – character of pilosis of a pubis; V – the maximum excess; VI – times of entry into a strip of a conditional and physiological rhythm after a marriage (pays off on time of stabilization of sexual activity; VII – time of entry into a strip of a conditional and physiological rhythm – absolute age. The statistical analysis was carried out in the environment of Statistica 12 Trial, with calculation of criterion of Chi-square with Yates,s amendment and approximation of variables. It is established that at patients with a perforative ulcer various disharmonies of adolescenty development are noted. Aged from 18 till 28 years (before marriage the perforative ulcer meets against a retardation of sexual development, high sexual activity and the periods of abstinency. Aged from 28 till 56 years the perforative ulcer proceeds against the average sexual constitution (breaking the adolescenty of manifestations with high excess practice of sexual intercourses and breaking the adolescenty of manifestations. At patients 57 years are more senior perforation arises against the high sexual constitution and activity. 

  13. Andrological aspects of the perforative ulcer of the duodenum

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    M. A. Nurtdinov

    2015-04-01

    Full Text Available Authors on a scale of vector definition of the sexual constitution investigated 115 patients with a perforative ulcer of a duodenum, in three age groups: the 1st from 18 to 28 years, the 2nd from 29 to 56 years and the 3 rd is more senior than 57 years. In this scale the following criteria are defined: the I vector – awakenings a libido; II – age of the first ejaculation; III – a trochanteric index (growth relation to foot length; IV – character of pilosis of a pubis; V – the maximum excess; VI – times of entry into a strip of a conditional and physiological rhythm after a marriage (pays off on time of stabilization of sexual activity; VII – time of entry into a strip of a conditional and physiological rhythm – absolute age. The statistical analysis was carried out in the environment of Statistica 12 Trial, with calculation of criterion of Chi-square with Yates,s amendment and approximation of variables. It is established that at patients with a perforative ulcer various disharmonies of adolescenty development are noted. Aged from 18 till 28 years (before marriage the perforative ulcer meets against a retardation of sexual development, high sexual activity and the periods of abstinency. Aged from 28 till 56 years the perforative ulcer proceeds against the average sexual constitution (breaking the adolescenty of manifestations with high excess practice of sexual intercourses and breaking the adolescenty of manifestations. At patients 57 years are more senior perforation arises against the high sexual constitution and activity. 

  14. Solitary extramedullary plasmacytoma of the duodenum and pancreas: A case report and review of the literature

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    Ignjatović Mile

    2016-01-01

    Full Text Available Introduction. The extramedullary plasmacytomas (EMPs are rare tumors of plasma cell disorders which are rarely found in the duodenum. We presented a case of solitary EMPs involving the duodenum and pancreas successfully treated by surgical resection after failure of chemotherapy. Case report. A 55-year-old female with previously diagnosed solitary EMP of the duodenum was admitted to our institution after failure of three cycles of vincristine, adriablastine, dexamethasone (VAD chemotherapy regimen with an upper gastrointestinal obstruction. On admission computed tomography of the abdomen showed tumor in the region of the second part of duodenum and uncinate process of the pancreas with a complete duodenal obstruction. Intraoperatively a tumor formation was in the region of the second duodenal part, originated from the wall of duodenum with the total diameter of 7 x 5 cm, covering the entire circumference of duodenal wall leaded to a narrowing of duodenal lumen to the thigh gap with an upper gastrointestinal obstruction. Infiltration in the head of the pancreas and uncinate process were also found. The Whipple’s procedure was performed but postoperative course was complicated by rapidly refilling chylous ascites which was resolved 4 days after the surgery. Conclusion. Each patient with gastrointestinal EMPs should be considered separately and in timely manner, thus adequate treatment could provide local disease control.

  15. Resection of the angle of Treitz and distal diverticulization of the duodenum in penetrating abdominal injuries.

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    Ruso, Luis; Taruselli, Roberto; Metcalfe, Matthew; Maddern, Guy

    2004-01-01

    Access to the 4th part of the duodenum in the region of the ligament of Treitz can be very difficult. Primary repair or traditional duodenal diverticulization is often technically challenging for managing trauma at this location. Due to the frequent concomitant injuries and hemorrhage, a quick, simple and safe repair technique is highly desirable. 3 patients with penetrating injuries to the 4th part of the duodenum were managed by a technique affording good exposure, and involving linear stapling across the bowel proximal and distal to the site of injury, with a jejuno-duodenal anastomosis to the 2nd part of the duodenum, the proximal jejunum having been delivered through a window fashioned in the transverse mesocolon. All patients survived and suffered no complications of their duodenal repair. The technique described offers a relatively simple, apparently safe and effective approach to a difficult problem in trauma surgery. Copyright 2004 S. Karger AG, Basel

  16. Management of gallstone ileus with stone impacted in the ascending part of duodenum.

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    Kirac, Iva; Nikolić, Marko; Kulis, Tomislav; Kovacević, Dujo; Lovrencić-Prpić, Gordana; Kirac, Petar

    2010-09-01

    Gallstone ileus with impaction of gallstone in distal duodenum is an extremely rare complication of cholecystolithiasis. Gallstone ileus in itself accounts for less than 1% of these complications with 800 cases described so far. However, there are less than 15 cases described with the impaction of gallstone in distal duodenum. We report a case of an 84-year-old male patient in whom gallstone impacted in distal duodenum was found by ultrasonography and confirmed by computed tomography. Cholecystitis and a remaining large gallstone in the gallbladder were found intraoperatively, which made us opt for one-step procedure, i.e. lithotomy and fistula repair with cholecystectomy. Due to the rare position of impacted gallstone, we believe that cases like this should be reported in detail in order to generate enough data for establishing optimal treatment options.

  17. Development and distribution of mast cells and neuropeptides in human fetus duodenum

    Institute of Scientific and Technical Information of China (English)

    Xiao-Yu Chen; Xue-Mei Jia; You-Su Jia; Xiao-Rong Chen; Hui-Zhu Wang; Wei-Qin Qi

    2004-01-01

    AIM: To study the developmental regularities and heterogeneity of mast cells (MC) in human fetus duodenum and the distribution and developmental regularities of substance P(SP), calcitonin gene-related peptide (CGRP)-immunoreactive (IR) peptidergic nerves in fetus duodenum,as well as the relationship between MC, SP and CGRP- IR peptidergic nerves.METHODS: Duodena from 21 cases of human fetus and one term infant were stained by hematoxylin-eosin (HE),toluidine blue (TB) and immunohistochemical avidin-biotinylated peroxidase complex (ABC) method.RESULTS: Lobe-shape intestinal villi in duodenum were already developed at the twelfth week. At the 21st wk,muscular mucosa appeared gradually, and four layers were observed in the wall of duodenum. TB staining showed that the granules in the immature MC were pale violet,while the mature MC were strong violet in color by TB staining. Connective tissue MC (CTMC) appeared occasionally in submucosa and muscular layer of duodenum at the16th wk. While the mucosa MC (MMC) appeared at the18th wk. At the 22nd wk, both CTMC and MMC were activated, and distributed in the surrounding blood vessels and ganglions. The verge of some MC were unclear, and showed degranular phenomena. At the 14th wk, SP and CGRP-IR nerve fibers and cells appeared in the myenteric and submucous plexuses in small intestine, and the responses were turn strongly. Neurons were light to deep brown, and nerve fibers were present as varicose and liner profiles. On the corresponding site of serial sections, SP and CGRP immunohistochemical reactions were coexisted in one nerve fiber or cell. Some of MC showed SP and CGRP-IR positive staining.CONCLUSION: There are two heterogeneous kinds of MC in duodenum, MMC and CTMC. MC might play an important role in regulating blood circulation and sensation.

  18. Postprandial fullness correlates with rapid inflow of gastric content into duodenum but not with chronic gastritis

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    Yamamichi Nobutake

    2011-12-01

    Full Text Available Abstract Background The aim of this study is evaluating the correlation of postprandial fullness with chronic gastritis or rapid inflow of gastric content into duodenum, based on double-contrast barium X-ray imaging. Methods 253 healthy subjects who underwent upper gastrointestinal barium X-ray examination were analyzed. Chronic gastritis was judged from mucosal atrophy and hypertrophic thickened folds on barium X-ray images. For the gastric excretion, the tips of barium flow on the single-contrast frontal barium X-ray images of the stomach were classified into four categories; V type (all the barium remained in the stomach, V-H type (some barium had flowed into the duodenum but the tip of barium remained in the proximal half of the duodenal bulb, H-V type (some barium had flowed into the duodenum and the tip of barium was in the distal half of duodenal the bulb, but no barium was observed in the descending part of the duodenum, and H type (some barium had flowed into the descending part of the duodenum. The chi-square test and Cochran-Mantel-Haenzel test were used for evaluation. Results Chronic gastritis was observed in 72 subjects, among which 21 subjects (29.2% presented with postprandial fullness. For the remaining 181 subjects without chronic gastritis, 53 subjects (29.3% complained of postprandial fullness. There is no significant correlation between chronic gastritis and postprandial fullness (p = 0.973. For the rapid flow of gastric content into duodenum, all the 253 subjects comprised 136 subjects with V type (in the stomach, 40 subjects with V-H type (in the proximal half of the duodenal bulb, 21 subjects with H-V type (in the distal half of the duodenal bulb, and 56 subjects with H type (in the descending part of the duodenum. Postprandial fullness was present in 30 subjects with V type (22.1%, 9 subjects with V-H type (22.5%, 8 subjects with H-V type (38.1%, and 27 subjects with H type (48.2%. There is a distinct correlation between

  19. Postprandial fullness correlates with rapid inflow of gastric content into duodenum but not with chronic gastritis

    Science.gov (United States)

    2011-01-01

    Background The aim of this study is evaluating the correlation of postprandial fullness with chronic gastritis or rapid inflow of gastric content into duodenum, based on double-contrast barium X-ray imaging. Methods 253 healthy subjects who underwent upper gastrointestinal barium X-ray examination were analyzed. Chronic gastritis was judged from mucosal atrophy and hypertrophic thickened folds on barium X-ray images. For the gastric excretion, the tips of barium flow on the single-contrast frontal barium X-ray images of the stomach were classified into four categories; V type (all the barium remained in the stomach), V-H type (some barium had flowed into the duodenum but the tip of barium remained in the proximal half of the duodenal bulb), H-V type (some barium had flowed into the duodenum and the tip of barium was in the distal half of duodenal the bulb, but no barium was observed in the descending part of the duodenum), and H type (some barium had flowed into the descending part of the duodenum). The chi-square test and Cochran-Mantel-Haenzel test were used for evaluation. Results Chronic gastritis was observed in 72 subjects, among which 21 subjects (29.2%) presented with postprandial fullness. For the remaining 181 subjects without chronic gastritis, 53 subjects (29.3%) complained of postprandial fullness. There is no significant correlation between chronic gastritis and postprandial fullness (p = 0.973). For the rapid flow of gastric content into duodenum, all the 253 subjects comprised 136 subjects with V type (in the stomach), 40 subjects with V-H type (in the proximal half of the duodenal bulb), 21 subjects with H-V type (in the distal half of the duodenal bulb), and 56 subjects with H type (in the descending part of the duodenum). Postprandial fullness was present in 30 subjects with V type (22.1%), 9 subjects with V-H type (22.5%), 8 subjects with H-V type (38.1%), and 27 subjects with H type (48.2%). There is a distinct correlation between postprandial

  20. Features of Clinical Course of Perforated Ulcers of Stomach and Duodenum in Children

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    A.G. Zaporozhchenko

    2013-12-01

    Full Text Available We examined 52 patients aged from 14 to 17 years with perforated ulcer of stomach and the duodenum, operated using laparoscopic and open ways. It is established, that perforated ulcer of stomach and the duodenum in children onset suddenly, the are characterized by absence of «ulcer anamnesis» and clinical picture of acute peritonitis in 77 % of children. Maximum efficiency of diagnosis of perforated gastric and duodenal ulcers is achieved by using a comprehensive diagnostic program that includes the use of phased diagnostic measures in accordance with their resolution in each case.

  1. Laparoscopic Pancreaticoduodenectomy for the Management of Localized Crohn's Disease of the Duodenum.

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    Xingjun, Guo; Feng, Zhu; Min, Wang; Renyi, Qin

    2016-08-01

    Crohn's disease of the duodenum is an uncommon condition. Our case was an extremely rare manifestation of Crohn's disease, who presented with obstruction of the pylorus and the first and the second parts of the duodenum. Because of the severity of the obstruction, he underwent laparoscopic pancreaticoduodenectomy. Postoperative pancreatic leakage and bowel fistula were not observed, and there was no morbidity during the follow-up period. There was also no disturbance in digestive function, postoperatively. This is the first case employing laparoscopic pancreaticoduodenectomy to cure benign lesions leading to duodenal obstruction. Minimally invasive laparoscopic pancreaticoduodenectomy technology shows a very big advantage in treating this rare benign Crohn's disease.

  2. 5-Hydroxytryptamine Induces Electrogenic Secretion in the Duodenum of Gerbil (Gerbillus cheesmani

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    Fawzia Y. Al-Balool

    2007-01-01

    Full Text Available The effect of serosally added 5-hydroxytryptamine (5-HT 100 µ­M on the short circuit-current (Isc across duodenum taken from fed, starved (4 days, water ad lib and undernourished (50% control food intake for 21 days gerbils (Gerbillus cheesmani were investigated. The effect of the neurotoxin, tetrodotoxin (TTX 10 µM and atropine (100 µ­M on the maximum increase in Isc induced by 5-HT were also studied. The 5-HT-induced Isc were higher in unstripped than in the stripped sheets in the three feeding conditions. TTX reduced the maximum increase in Isc induced by 5-HT across stripped and unstripped sheets taken from fed, starved and undernourished gerbils. Atropine decreased the 5-HT-induced Isc of stripped sheets in the three feeding conditions and it also decreased the 5-HT-induced Isc in unstripped sheets in fed duodenum. Therefore, the duodenal response to 5-HT occur partly by activation of a nonneural pathway and partly by activating electrogenic ion transport via muscarinic neural mechanism. It also showed that the 5-HT-induced Isc was chloride-dependent in fed duodenum and were chloride and bicarbonate dependent in the duodenum taken from starved and undernourished gerbil The results also showed that the increase in 5-HT-induced Isc as a results of starvation and undernourishment were TTX-sensitive and both chloride and bicarbonate dependent.

  3. [A case of tuberculous lymphadenitis found as an abdominal mass penetrating the duodenum].

    Science.gov (United States)

    Tokiwa, Kayoko; Hozawa, Shigenari; Kojima, Seiichirou; Kobayashi, Keinji; Watanabe, Kenichi; Mine, Tetsuya; Kijima, Hiroshi; Imai, Yutaka; Miyachi, Hayato

    2006-08-01

    A 42-year-old woman who complained of epigastralgia was referred to our hospital because of an abdominal mass found by ultrasonography. CT and MRI scans revealed that the abdominal mass, 4 cm in diameter, located on the left side of the right kidney. Gastroduodenoscopy detected a deep ulcerative lesion covered with a yellowish coat in the second portion of duodenum. A sonolucent area at the bottom of the ulcerative lesion seemed to expand to the abdominal mass on ultrasonic endoscopy. Tuberculosis was one possible differential diagnosis. Pathological examination including Ziehl-Neelsen staining using biopsy specimens taken from the bottom of the ulcerative lesion did not show tuberculosis infection. However, polymerase chain reaction analysis using the biopsy specimens revealed that gene expression of tuberculosis was positive. We determined that tuberculous lymphadenitis penetrated the duodenum forming an ulcerative lesion in the duodenum. The administration of anti-tubercular medicine for 6 months male the abdominal mass disappeared and the duodenal ulcerative lesion formed an ulcer scar. We report here a case of tuberculous lymphadenitis penetrating the duodenum which was successfully treated by conservative therapy without surgical treatment.

  4. Different mechanisms of actions of genistein and quercetin on spontaneous contractions of rabbit duodenum

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    Diego Santos-Fagundes

    2015-07-01

    Full Text Available Flavonoids are known to relax precontracted intestinal smooth muscle and delay intestinal transit or intestinal peristalsis. The aim of this study was to determine the effects of genistein and quercetin on spontaneous contractions of rabbit duodenum in vitro in an organ bath. Genistein and quercetin (0.1-10 µM reduced the amplitude of spontaneous contractions in the longitudinal and circular smooth muscle of rabbit duodenum, but they did not modify the frequency. Bay K8644 (L-type Ca2+ channel activator, apamin, charybdotoxin, and tetraetylammonium (K+ channel blockers reverted the inhibition of amplitude of spontaneous contractions induced by genistein in longitudinal and circular smooth muscle. H-89 (protein kinase A inhibitor antagonized the reduction of the amplitude of spontaneous contractions induced by quercetin in longitudinal and circular smooth muscle of duodenum, while 2,5-dideoxiadenosine (adenylyl cyclase inhibitor reverted only the reduction of the amplitude in circular smooth muscle. In conclusion, genistein and quercetin reduce the spontaneous contractions in the duodenum by different mechanisms of actions. The effect of genistein would be mediated by Ca2+ and K+ channels, while the effect of quercetin would be mediated by cAMP and protein kinase A.

  5. Fulminant phlegmonitis of the esophagus, stomach, and duodenum due to Bacillus thuringiensis

    Science.gov (United States)

    Matsumoto, Hisatake; Ogura, Hiroshi; Seki, Masafumi; Ohnishi, Mitsuo; Shimazu, Takeshi

    2015-01-01

    We report a case of phlegmonitis of the esophagus, stomach, and duodenum in patient in an immunocompromised state. Culture of gastric juice and blood yielded Bacillus thuringiensis. This case showed that even low-virulence bacilli can cause lethal gastrointestinal phlegmonous gastritis in conditions of immunodeficiency. PMID:25834344

  6. Fulminant phlegmonitis of the esophagus, stomach, and duodenum due to Bacillus thuringiensis.

    Science.gov (United States)

    Matsumoto, Hisatake; Ogura, Hiroshi; Seki, Masafumi; Ohnishi, Mitsuo; Shimazu, Takeshi

    2015-03-28

    We report a case of phlegmonitis of the esophagus, stomach, and duodenum in patient in an immunocompromised state. Culture of gastric juice and blood yielded Bacillus thuringiensis. This case showed that even low-virulence bacilli can cause lethal gastrointestinal phlegmonous gastritis in conditions of immunodeficiency.

  7. Perforated Duodenum – An Unusual Etiology of Fournier's Disease: A Case Report

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    Yung-Chin Lee

    2003-12-01

    Full Text Available Fournier's disease, a form of necrotizing fasciitis, is a rapidly progressing subcutaneous infection of the male genitalia. We report a case of Fournier's disease with the unusual etiology of a perforated duodenum. This patient suffered from progressive right scrotal swelling after percutaneous transhepatic cholangeal drainage. Scrotal exploration revealed a large abscess with muscle necrosis that had spread up to the right retroperitoneal space. Radiologic studies and second abdominal exploration documented the origin as a perforated duodenum. The pus distribution in this case suggested that the infection process differed from that in previous reports. In future cases of Fournier's disease involving previous abdominal events, we recommend that abdominal origins be carefully surveyed before scrotal exploration.

  8. Pedunculated Brunner's gland hamartoma of the duodenum causing upper gastrointestinal hemorrhage

    Institute of Scientific and Technical Information of China (English)

    Shoji Hirasaki; Motoharu Kubo; Atsushi Inoue; Yasuyuki Miyake; Hisako Oshiro

    2009-01-01

    A case of pedunculated Brunner's gland hamartoma (BGH) of the duodenum causing upper gastrointestinal (GI) hemorrhage is reported. The patient was a 47-year-old man who visited our hospital for further evaluation of tarry stools and shortness of breath.Endoscopic examination of the upper digestive tract revealed a large peduncular polyp with bleeding,about 30 mm in diameter, arising from the wall of the second portion of the duodenum. GI bleeding occurred from the base of the stalk of the polyp. Endoscopic polypectomy was performed. Histological examination of the specimen revealed that the main body of the polyp contained several lobules of mature Brunner'gland with areas of cystic dilatation. The surface epithelium consisted of normal duodenal mucosa with areas of focal ulceration. This polyp was diagnosed as a BGH. The symptom of tarry stools resolved after endoscopic resection. Our case shows that treatment is necessary for duodenal BGH if GI bleeding occurs.

  9. A gastrointestinal stromal tumor of the duodenum masquerading as a pancreatic head tumor

    Institute of Scientific and Technical Information of China (English)

    Sung Ho Kwon; Jung Woo Shin; Neung Hwa Park; Do Ha Kim; Hee Jeong Cha; Seok Won Jung; Byung Chul Kim; Jae Serk Park; In Du Jeong; Jong Hwa Lee; Yang Won Nah; Sung Jo Bang

    2007-01-01

    Gastrointestinal stromal tumor (GIST) represents the most common kind of mesenchymal tumor that arises from the alimentary tract. GIST is currently defined as a gastrointestinal tract mesenchymal tumor showing CD117 (c-kit protein) positivity at immunohistochemistry.Throughout the whole length of the gastrointestinal tract, GIST arises most commonly from the stomach followed by the small intestine, the colorectum, and the esophagus. Only 3%-5% of GISTs occur in the duodenum, and especially, if GIST arises from the C loop of the duodenum, it can be difficult to differentiate from the pancreas head mass because of its anatomical proximity. Here, we report a case of duodenal GIST,which was assessed as a pancreatic head tumor preoperatively.

  10. Entero-hepatic pathobiology: histopathology and semi-quantitative bacteriology of the duodenum

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    NMK Ito

    2004-03-01

    Full Text Available Duodenum swabs were harvested from 80 broilers in 15 flocks of 8 broiler farms and were submitted to bacteriological evaluation. Five broiler breeders were sampled to obtain comparative data. Macro and microscopic lesions in the gut and the liver were evaluated in all birds submitted to swab harvesting and also broilers of two slaughter plants. Semiquantitative bacterial examination in duodenum swabs demonstrated higher microorganism growth in blood agar than in MacConkey medium. Staphylococcus sp, Enterococcus sp and Escherichia coli were found in some broiler flocks. An increased cellularity of the muscular mucosae of duodenum villi and/or the presence of some gametocytes or schizonts of coccidia were found in the lesioned gut in addition to high bacterial growth in blood agar. In some flocks, enlarged yellowish livers with vacuolated hepatocytes and/or increased reticuloendothelial response were seen in some birds. Liver and gut samples were collected in two slaughter plants in order to establish a pattern of response in broilers after withdrawal of growth promoters. Livers with macroscopic lesions presented microscopic lesions in the parenchyma such as increased leukocyte response in the sinusoidal space, vacuolar degeneration of hepatocytes or focal necrosis in the perilobular region, increased size or number of lymphoid nests in the perilobular region, the perivascular space and surrounding the biliary duct and increased extramedular granulocytopoiesis. Duodenum with hyperemic patches showed increased cellular response in the muscular mucosa of villi. These observations support the concept that semi-quantitative bacteriology and liver lesion evaluation could be used as a model to study gut bacterial imbalance in chickens.

  11. Adenocarcinoma of the third portion of the duodenum in a man with CREST syndrome

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    Fragulidis Georgios

    2008-10-01

    Full Text Available Abstract Background CREST (Calcinosis, Raynaud's phenomenon, Esophageal dysmotility, Sclerodactyly and Telangiectasias syndrome has been rarely associated with other malignancies (lung, esophagus.This is the first report of a primary adenocarcinoma of the third portion of the duodenum in a patient with CREST syndrome. Case presentation A 54-year-old male patient with CREST syndrome presented with colicky postprandial pain of the upper abdomen, diminished food uptake and a 6-Kg-body weight loss during the previous 2 months. An ulcerative lesion in the third portion of the duodenum was revealed during duodenoscopy, with a diagnosis of adenocarcinoma on biopsy specimen histology. The patient underwent a partial pancreatoduodenectomy. No adjuvant therapy was instituted and follow-up is negative for local recurrence or metastases 21 months postoperatively. Conclusion CREST syndrome has been associated with colon cancer, gastric polyps, familial adenomatous polyposis (FAP syndrome and Crohn's disease; however, this is the first report of a primary adenocarcinoma of the duodenum in a patient with CREST syndrome. However, any etiologic relationship remains to be further investigated.

  12. Tebal Struktur Histologis Duodenum Ayam Pedaging yang Diberi Kombinasi Tylosin dan Gentamicin

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    I Gede Gilang Ikra Raditya

    2013-12-01

    Usaha ternak broiler, sejak tahun 1998 semakin menonjol perannya dalam mempersempit kesenjangan terhadap meningkatnya kebutuhan akan daging. Daging ayam broiler dipilih sebagai salah satu alternatif, karena kita tahu bahwa ayam broiler sangat efisen diproduksi. Tujuan dilakukan penelitian ini adalah untuk mengetahui tebal struktur histologis duodenum ayam yang diberi kombinasi tylosin dan gentamicin. Metode yang dipakai pada penelitian ini menggunakan 32 ayam pedaging yang di bagi dalam 4 kelompok yang di mana masing masing kelompok terbagi atas 8 ayam. Hasil penelitian ini sendiri adalah ketebalan struktur histologis duodenum pada kontrol (P0 rata-rata 7,2 ?m, perlakuan P1 rata-rata 7,2 ?m, perlakuan P2 rata-rata adalah 7,6 ?m, dan perlakuan P3 rata-rata adalah 7,9 ?m. P3 lebih tebal dari P2 dan P2 lebih besar dari P1 dan P0. Kesimpulan dari penelitian ini adalah kombinasi tylosin dan gentamicin ini efektif untuk menyeimbangkan flora normal yang ada di dalam duodenum sehingga membuat pertumbuhan ayam menjadi sempurna.

  13. Extramedullary plasmocytoma associated with a massive deposit of amyloid in the duodenum

    Institute of Scientific and Technical Information of China (English)

    Fabiana Pirani Carneiro; Maria de Nazareth Machado Sobreira; Livia Bravo Maia; Alesso Cervantes Sartorelli; Luiz Eduardo de Almeida Prado Franceschi; Mauro Brito Brandao; Bárbara Wosnjuk Calaca; Fernando Silva Lustosa; Joao Vieira Lopes

    2009-01-01

    We report a rare case of extramedullary plasmocytoma associated with a massive deposit of amyloid in the duodenum. A 72-year-old Japanese man was admitted to our hospital presenting with a 3-mo history of epigastric pain, vomiting and weight loss. On computed tomography (CT) a wall thickening of the fourth part of the duodenum was observed. Multiple biopsies obtained from the lesion showed infiltration of plasma cells and lymphocytes, but they were not conclusive. The patient underwent resection of the lesion and, on histopathological examination, the lesion consisted of a dense and diffuse infiltrate of plasma cells and a few admixed lymphocytes with reactive follicles extending to the muscular propria. An extensive deposition of amyloid was also observed. Immunohistochemical stains revealed that a few plasmacytoid cells showed λ light chain staining, though most were κ light chain positive. These cells also were positive for CD138 and CD56 but negative for CD20 and CD79. The findings were consistent with extramedullary plasmocytoma associated with a massive deposit of amyloid in duodenum. A subsequent workup for multiple myeloma was completely negative. The patient showed no signs of local recurrence or dissemination of the disease after 12 mo follow-up. Because of the association of plasmocytoma and amyloidosis, the patient must be followed up because of the possible systemic involvement of the neoplasm and amyloidosis in future.

  14. Multiple carcinoids in the duodenum, pancreas and stomach accompanied with type A gastritis: A case report

    Institute of Scientific and Technical Information of China (English)

    Takeo Bamba; Shin-ichi Kosugi; Tatsuo Kanda; Toshihiro Tsubono; Yasuo Sakai; Nobuyuki Musha; Noriko Ishihara; Katsuyoshi Hatakeyama

    2007-01-01

    We report a case of multiple duodenal, pancreatic, and gastric carcinoids. A 67-year old woman was admitted to our hospital for treatment of a duodenal carcinoid.Laboratory tests revealed that the patient was associated with macrocytic anemia and hypergastrinemia, and type A gastritis was shown by gastrofiberscopy.During surgery, another tumor was incidentally found in the head of the pancreas. The tumors in the duodenum and pancreas were completely excised by pancreatoduodenectomy and immunohistologically diagnosed as gastrin-and serotonin-producing carcinoids,respectively. Pathological examination revealed that in addition to the grossly found carcinoids, there were subclinical carcinoids, one of which was an endocrine cell micronest, located in the stomach and duodenum.The tumors in the duodenum, pancreas, and stomach showed different characteristics from one another morphologically and immunochemically. Although no definitive evidence has been obtained, some sort of genetic anomaly may have been involved in this case,and hypergastrinemia due to duodenal gastrinoma may induce multiple gastric carcinoids.

  15. A large gastrointestinal stromal tumor of the duodenum: a case report

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    Al-Ahmad Firas

    2011-09-01

    Full Text Available Abstract Introduction Gastrointestinal stromal tumors of the duodenum are uncommon. They can reach a large size. Diagnosis can be elusive and managing them can be difficult. Our case report aims to increase awareness and highlight some issues related to the diagnosis and management of duodenal gastrointestinal stromal tumors. Case presentation We present the case of a 38-year-old Middle Eastern woman with a large, slowly-growing gastrointestinal stromal tumor of the duodenum. Her complaints were minor epigastric discomfort and swelling. A pancreaticoduodenectomy with complete tumor excision was performed. She was doing very well with no evidence of disease recurrence when she was last seen 34 months after her operation. Conclusion Gastrointestinal stromal tumors of the duodenum should be suspected in any patient with a duodenal wall mass. Extramural growth and central ulceration with or without bleeding should alert the endoscopist to the possibility of a duodenal gastrointestinal stromal tumor diagnosis. There is more than one surgical approach available; however, complete surgical excision, with negative margins, is the absolute requirement. Preoperative imatinib mesylate can be considered in unresectable or borderline resectable cases.

  16. Is it safe to perform endoscopic band ligation for the duodenum? A pilot study in ex vivo porcine models.

    Science.gov (United States)

    Kakutani, Hiroshi; Sasaki, Shigemasa; Ueda, Kaoru; Takakura, Kazuki; Sumiyama, Kazuki; Imazu, Hiroo; Hino, Syoryoku; Kawamura, Muneo; Tajiri, Hisao

    2013-04-01

    In the digestive tract, endoscopic band ligation (EBL) has been routinely used for the treatment of variceal bleeding and superficial malignancies. In recent years, endoscopic treatments for duodenal varices, adenoma, and cancer have also actively incorporated EBL. Although there have been a number of reports on the risks associated with the use of EBL in the esophagus, stomach, and colon, few studies have focused on EBL in the duodenum. We performed EBL procedures to evaluate the risks associated with the use of EBL in the duodenum. Overall, EBLs were performed at nine sites in duodenum sampled from a pig immediately after sacrifice. Submucosal saline injections were placed in three of the nine studied sites. Regardless of saline injection, the full thickness of the duodenal wall was ligated in all attempts. Routine EBL is not recommended in the duodenum because the risk of perforation is unacceptably high.

  17. Microarray based gene expression analysis of Sus Scrofa duodenum exposed to zearalenone: significance to human health.

    Science.gov (United States)

    Braicu, Cornelia; Cojocneanu-Petric, Roxana; Jurj, Ancuta; Gulei, Diana; Taranu, Ionelia; Gras, Alexandru Mihail; Marin, Daniela Eliza; Berindan-Neagoe, Ioana

    2016-08-17

    Zearalenone (ZEA) is a secondary metabolite produced by Fusarium species. ZEA was proved to exert a wide range of unwanted side effects, but its mechanism of action, particularly at duodenum levels, remains unclear. In our study based on the microarray technology we assessed the alteration of gene expression pattern Sus scrofa duodenum which has been previously exposed to ZEA. Gene expression data was validated by qRT-PCR and ELISA. The gene expression data were further extrapolated the results to their human orthologues and analyzed the data in the context of human health using IPA (Ingenuity Pathways Analysis). Using Agilent microarray technology, we found that gene expression pattern was significantly affected by ZEA exposure, considering a 2-fold expression difference as a cut-off level and a p-value < 0.05. In total, we found 1576 upregulated and 2446 downregulated transcripts. About 1084 genes (764 downregulated and 751 overexpressed) were extrapolated to their human orthologues. IPA analysis showed various altered key cellular and molecular pathways. As expected, we observed a significant alteration of immune response related genes, MAPK (mitogen activate protein kinases) pathways or Toll-Like Receptors (TLRs). What captured our attention was the modulation of pathways related to the activation of early carcinogenesis. Our data demonstrate that ZEA has a complex effect at duodenum level. ZEA is able to activate not only the immune response related genes, but also those relate to colorectal carcinogenesis. The effects can be more dramatic when connected with the exposure to other environmental toxic agents or co-occurrence with different microorganisms.

  18. Sporadic versus hereditary gastrinomas of the duodenum and pancreas: Distinct clinico-pathological and epidemiological features

    Institute of Scientific and Technical Information of China (English)

    Martin Anlauf; Wolfram T Knoefel; Henning Dralle; Paul Komminoth; Philipp U Heitz; Aurel Perren; Günter Kl(o)ppel; Nele Garbrecht; Tobias Henopp; Anja Schmitt; Regina Schlenger; Andreas Raffel; Markus Krausch; Oliver Gimm; Claus F Eisenberger

    2006-01-01

    Gastrinomas are defined as gastrin secreting tumors that are associated with Zollinger-Ellison syndrome (ZES). ZES is characterized by elevated fasting gastrin serum levels, positive secretin stimulation test and clinical symptoms such as recurrent peptic ulcer disease, gastroesophageal reflux disease and occasional diarrhea. Genetically, nonhereditary (sporadic) gastrinomas are distinguished from hereditary gastrinomas, which are associated with multiple endocrine neoplasia type 1 (MEN1) syndrome. In general, duodenal gastrinomas are small and solitary if they are sporadic and multiple as well as hereditary. The sporadic gastrinomas occur in the duodenum or in the pancreas while the hereditary gastrinomas almost all occur in the duodenum. Our series of 77 sporadic duodenal neuroendocrine tumors (NETs) includes 18 patients (23.4%) with gastrinomas and ZES. Of 535 sporadic NETs in the pancreas collected from the NET archives of the departments of pathology (4.5%) suffered from sporadic pancreatic gastrinomas and ZES. These NETs have to be distinguished from tumors with immunohistochemical positivity for gastrin but without evidence of ZES. An additional 19 patients suffered from MEN1 and ZES. These patients showed exclusively duodenal gastrinomas, but not pancreatic gastrinomas. The prognosis of sporadic and MEN1-associated duodenal gastrinomas is better than that of pancreatic gastrinomas, since they progress slowly to liver metastasis. In summary, sporadic and MEN1-associated gastrinomas in the duodenum and pancreas show different clinico-pathological and genetic features. The incidence of sporadic duodenal gastrin-producing tumors is increasing, possibly due to optimized diagnostic procedures. In contrast, pancreatic MEN1-associated gastrinomas seem to be extremely rare. A considerable subset of tumors with immunohistochemical expression of gastrin but without evidence of ZES should be designated as functionally inactive NETs expressing gastrin, but not as

  19. Management of Locally Advanced Renal Cell Carcinoma with Invasion of the Duodenum

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    Andrew T. Schlussel

    2013-01-01

    Full Text Available Renal cell carcinoma (RCC is rare but aggressive, with greater than 20% of patients presenting with stage III or IV, disease. Surgical resection of the primary tumor regardless of stage is the treatment of choice, and en bloc resection of involved organs provides the only potential chance for cure. This case report describes a patient with metastatic right-sided RCC with invasion of the inferior vena cava and duodenum managed by en block resection and pancreaticoduodenectomy. This report will review the workup and treatment of locally advanced RCC, as well as the role of cytoreductive nephrectomy in the setting of metastatic disease.

  20. Cadmium inhibition of vitamin D-mediated responses in organ-cultured embryonic chick duodenum

    Energy Technology Data Exchange (ETDEWEB)

    Corradino, R.A.

    1979-01-01

    When added to the medium, cadmium inhibits 1..cap alpha..,25-dihydroxycholecalciferol-mediated responses in the organ-cultured embryonic chick duodenum: decreases induction of a specific calcium-binding protein (CaBP), prevents the elevation of alkaline phosphatase activity, and reduces the ability of the tissue to absorb radiocalcium at the mucosal surface. The cadmium effect is clearly not generalized cytotoxicity. These results may be taken as evidence that cadmium can interfere with vitamin D action at the level of the target organ itself and is not necessarily secondary to alteration in vitamin D metabolism.

  1. Central vagal stimulation activates enteric cholinergic neurons in the stomach and VIP neurons in the duodenum in conscious rats.

    Science.gov (United States)

    Yuan, Pu-Qing; Kimura, Hiroshi; Million, Mulugeta; Bellier, Jean-Pierre; Wang, Lixin; Ohning, Gordon V; Taché, Yvette

    2005-04-01

    The influence of central vagal stimulation induced by 2h cold exposure or intracisternal injection of thyrotropin-releasing hormone (TRH) analog, RX-77368, on gastro-duodenal enteric cholinergic neuronal activity was assessed in conscious rats with Fos and peripheral choline acetyltransferase (pChAT) immunoreactivity (IR). pChAT-IR was detected in 68%, 70% and 73% of corpus, antrum and duodenum submucosal neurons, respectively, and in 65% of gastric and 46% of duodenal myenteric neurons. Cold and RX-77368 induced Fos-IR in over 90% of gastric submucosal and myenteric neurons, while in duodenum only 25-27% of submucosal and 50-51% myenteric duodenal neurons were Fos positive. In the stomach, cold induced Fos-IR in 93% of submucosal and 97% of myenteric pChAT-IR neurons, while in the duodenum only 7% submucosal and 5% myenteric pChAT-IR neurons were Fos positive. In the duodenum, cold induced Fos in 91% of submucosal and 99% of myenteric VIP-IR neurons. RX-77368 induces similar percentages of Fos/pChAT-IR and Fos/VIP-IR neurons. These results indicate that increased central vagal outflow activates cholinergic neurons in the stomach while in the duodenum, VIP neurons are preferentially stimulated.

  2. Subcellular distribution of nitric oxide synthase isoforms in the rat duodenum

    Institute of Scientific and Technical Information of China (English)

    Petra Talapka; Nikolett Bódi; Izabella Battonyai; (E)va Fekete; Mária Bagyánszki

    2011-01-01

    AIM:To study the cell-type specific subcellular distribution of the three isoforms of nitric oxide synthase (NOS) in the rat duodenum. METHODS:Postembedding immunoelectronmicroscopy was performed,in which primary antibodies for neuronal NOS (nNOS),endothelial NOS (eNOS),and inducible NOS (iNOS),were visualized with protein A-gold-conjugated secondary antibodies.Stained ultrathin sections were examined and photographed with a Philips CM10 electron microscope equipped with a MEGAVIEW II camera.The specificity of the immunoreaction in all cases was assessed by omitting the primary antibodies in the labeling protocol and incubating the sections only in the protein A-gold conjugated secondary antibodies. RESULTS:Postembedding immunoelectronmicroscopy revealed the presence of nNOS,eNOS,and iNOS immunoreactivity in the myenteric neurons,the enteric smooth muscle cells,and the endothelium of capillaries running in the vicinity of the myenteric plexus of the rat duodenum.The cell type-specific distributions of the immunogold particles labeling the three different NOS isozymes were revealed.In the control experiments,in which the primary antiserum was omitted,virtually no postembedding gold particles were observed. CONCLUSION:This postembedding immunoelectronmicroscopic study provided the first evidence of celltype- specific differences in the subcellular distributions of NOS isoforms.

  3. Two synchronous somatostatinomas of the duodenum and pancreatic head in one patient

    Institute of Scientific and Technical Information of China (English)

    Radoje B (C)olovi(c); Slavko V Mati(c); Marjan T Micev; Nikica M Grubor; Henry Dushan Atkinson; Stojan M Latin(c)i(c)

    2009-01-01

    Somatostatinomas are extremely rare neuroendocrine tumors of the gastrointestinal tract, first described in the pancreas in 1977 and in the duodenum in 1979. They may be functional and cause somatostatinoma or inhibitory syndrome, but more frequently are non-functioning pancreatic endocrine tumors that produce somatostatin alone. They are usually single, malignant, large lesions, frequently associated with metastases, and generally with poor prognosis. We present the unique case of a 57-year-old woman with two synchronous non-functioning somatostatinomas, one solid duodenal lesion and one cystic lesion within the head of the pancreas, that were successfully resected with a pylorus-preserving Whipple's procedure. No secondaries were found in the liver, or in any of the removed regional lymph nodes. The patient had an uneventful recovery, and remains well and symptom-free at 18 mo postoperatively. This is an extremely rare case of a patient with two synchronous somatostatinomas of the duodenum and the pancreas. The condition is discussed with reference to the literature.

  4. Neuroendocrine Tumors in the Stomach, Duodenum, and Pancreas Accompanied by Novel MEN1 Gene Mutation.

    Science.gov (United States)

    Yang, Min A; Lee, Woong Ki; Shin, Hong Shik; Park, Sung Hyun; Kim, Byung Sun; Kim, Ji Woong; Cho, Jin Woong; Yun, So Hee

    2017-03-25

    Multiple endocrine neoplasia type 1 (MEN1) syndrome is a relatively rare disease, characterized by the occurrence of multiple endocrine tumors in the parathyroid and pituitary glands as well as the pancreas. Here, we report a case of MEN1 with neuroendocrine tumors (NETs) in the stomach, duodenum, and pancreas. A 53-year-old man visited our hospital to manage gastric NET. Five years prior to his visit, he had undergone surgery for incidental meningioma. His brother had pancreatic nodules and a history of surgery for adrenal adenoma. His brother's daughter also had pancreatic nodules, but had not undergone surgery. The lesion was treated by endoscopic submucosal dissection and diagnosed as a grade 1 NET. Another small NET was detected in the second duodenal portion, resected by endoscopic submucosal dissection, which was also diagnosed as a grade 1 NET. During evaluation, three nodules were detected in the pancreas, and no evidence of pituitary, parathyroid tumors, or metastasis was observed. After surgery, the pancreatic lesions were diagnosed as NETs, with the same immunohistochemical patterns as those of the stomach and duodenum. Genetic testing was performed, and a heterozygous mutation was detected in the MEN1 gene, which is located on 11q13.

  5. Togetherness of the Multiple Diverticulosis of the First Part of the Duodenum with Neurofibromatosis Type I: A Case Report

    Directory of Open Access Journals (Sweden)

    Ozgur Turk

    2014-04-01

    Full Text Available Duedonal diverticulosis is common in clinical practice. Most of duedonal diverticulosis is located at the second part of duodenum; incidence is reported as 67%, incidence of third and forth part is %20. Multiple diverticulosis of the first part of the duodenum is a rare case. There is not any knowledge togetherness of neurofibromatosis type 1 and duedonal diverticulosis. Here we reported incidentally diagnosed multiple diverticulosis of duodenum that is located in the first part in a neurofibromatosis type 1 patient with multiple neurofibromatosis nodules. Neurofibromatosis could affect various organs. Along the digestive system carcinoid tumors and neuroendocrine tumors are more encountered diseases. Endoscopic examinations and abdominal imaging may be useful for the diagnosis of gastrointestinal coexisting disease in neurofibromatosis type 1 . In conclusion neurofibromatosis type 1 could affect multiple systems and it should evaluate carefully.

  6. [6 years' personal experience with duodenum-sparing procedures in chronic pancreatitis].

    Science.gov (United States)

    Sváb, J; Fried, M; Bortlík, M; Lukás, M; Horejs, J

    2000-06-01

    The First Surgical Clinic of the First Medical Faculty Charles University and General Faculty Hospital Prague made operations of the pancreas ever since 1971. In the work sooner or later all approaches to surgical treatment of pancreatitis were reflected. The authors present a brief review of results and their own experience since 1994 when duodenum sparing operations were introduced. Indications for surgical treatment were based on the diagnosis by US, CT and ERCP, in exceptional cases MR, after evaluation by a pancreatologist, roentgenologist and surgeon. The group of patients with chronic pancreatitis was extended by 15 patients from a group operated because of preoperative suspicion of a malignant pancreatic tumour not confirmed during and after surgery. In those Whipple's operation was performed. The same operation was performed in three patients with chronic pancreatitis with serious changes in the area of the head of the pancreas. In 111 patients a drainage and duodenum sparing operation was performed. Of these in 46x according to Neger, 9x according to Frey, 10x modification of these operations, 37x Partington-Rochelle's procedure. The authors did not record postoperative complications after the classical Beger operation and the hospital stay was on average by five days shorter as compared with the classical method of Whipple. When evaluating postoperative complaints and problems (pain, malnutrition, physical constitution and social position) the authors recorded equally favourable results as after non-complicated duodenopancreatectomy. They varied, depending on the patient s co-operation round 87% while after longitudinal drainage of the duct a satisfactory result was recorded in 78% of the operated patients. The authors consider Beger's operation logical because of the removal of the main tissue mass of the head of the pancreas, responsible for pain, complications caused by fibrosis in the area round the bile duct and duodenum, responsible for the

  7. Noncontiguous finished genome sequence and description of Fusobacterium massiliense sp. nov. isolated from human duodenum

    Directory of Open Access Journals (Sweden)

    M. Mailhe

    2017-03-01

    Full Text Available The strain Marseille-P2749T (= CSUR P2749=DSM 103085 was isolated as part of culturomics study from a liquid duodenum sample from a French man. Bacterial cells were Gram-negative bacilli, fusiform shaped and non–spore forming, and they grew in microaerophilic and anaerobic atmosphere. Its genome is 1 809 169 bp long and contains 1646 protein-coding genes. The DNA G+C content was 27.33 mol%. This strain exhibited a 95.9% sequence similarity with Fusobacterium periodonticum, the phylogenetically closest species with standing in nomenclature. Strain Marseille-P2749T is suggested to be a novel species belonging to the genus Fusobacterium, for which the name Fusobacterium massiliense sp. nov. is proposed.

  8. The role of adrenergic receptors in the motility of duodenum and choledochoduodenal junction in the pig.

    Science.gov (United States)

    Blichowski, A; Andrzejewski, W; Gaszyński, W; Kozulski, W

    1977-01-01

    The role of adenergic receptors in the motility of duodenum and choledochoduodenal junction in the pig. Acta Physiol. Pol., 1977, 28 (6): 521-528. The choldeochoduodenal junction in the Vietnamese pig is functionally and anatomically a part of duodenal wall. In view of this, investigations were carried out for establishing the role of adrenergic receptors in the development of motor function of this part of the intestinal tract. The experiments were performed on domestic Vietnamese pigs (Sus scrofa domestica) and they showed that after stimulation of alpha and beta adrenergic receptors the motor activity of the duodenal muscular coat and the choledochoduodenal junction is inhibited. The obtained results suggest similar reactions of the adrenergic receptors in both examined parts of the intestinal tract in the pig.

  9. The role of mast cell in tissue morphogenesis. Thymus, duodenum, and mammary gland as examples.

    Science.gov (United States)

    Ribatti, Domenico; Crivellato, Enrico

    2016-02-01

    Mast cells (MCs) are strategically located at host/environment interfaces like skin, airways, and gastro-intestinal and uro-genital tracts. MCs also populate connective tissues in association with blood and lymphatic vessels and nerves. MCs are absent in avascular tissues, such as mineralized bone, cartilage, and cornea. MCs have various functions and different functional subsets of MCs are encountered in different tissues. However, we do not' know exactly what is the physiological function of MC. Most of these functions are not essential for life, as various MC-deficient strains of mice and rats seems to have normal life spans. In this review article, we have reported and discussed the literature data concerning the role of MCs in tissue morphogenesis, and in particular their role in the development of thymus, duodenum, and mammary gland.

  10. Gastrointestinal stromal tumour of the duodenum in a 7-year-old boy

    Energy Technology Data Exchange (ETDEWEB)

    Hughes, Jacqueline Ann; Reidy, John [Guy' s Hospital NHS Trust, Department of Radiology, London (United Kingdom); Cook, Jane Valmai [Epsom and St Helier' s Hospitals NHS Trust, Department of Radiology, Queen Mary' s Hospital for Children, Carshalton (United Kingdom); Said, Ahmid; Towu, Emmanuel [University Hospital Lewisham NHS Trust, Department of Paediatric Surgery, London (United Kingdom); Chong, Sonny K. [Epsom and St Helier' s Hospitals NHS Trust, Department of Gastrointestinal Medicine, Queen Mary' s Hospital for Children, Carshalton, Surrey (United Kingdom)

    2004-12-01

    We report a 7-year-old boy presenting with an acute upper gastrointestinal (GI) haemorrhage subsequently diagnosed to have a very rare duodenal gastrointestinal stromal tumour (GIST). Endoscopy, pertechnetate and red cell scans were negative. Abdominal US detected a 17-mm mass lesion of the third part of the duodenum. This was confirmed on CT and shown to be hypervascular on selective angiography. At laparotomy, a 20-mm submucosal duodenal lesion was found associated with mucosal ulceration. Immunohistochemical analysis revealed it to be positive for CD117 (c-KIT protein) consistent with a GIST. We emphasize the importance of a thorough abdominal US examination in children with GI haemorrhage and the consideration of GIST in the diagnosis after the common causes have been excluded. (orig.)

  11. Duodenum-derived fibromatosis that invaded the muscular layer of intestinal wall: A rare case report.

    Science.gov (United States)

    Li, Jiannan; Le, Hanxiang; Chai, Wei; Zhou, Yan; Jin, Lifang; Liu, Tongjun; Zhang, Kai

    2017-08-01

    Intraabdominal fibromatosis is a rare benign tumor that often affects the mesentery or retroperitoneum, and can infiltrate adjacent organs. However, it is rare for fibromatosis to arise from the small intestinal wall. A 27-year-old female with a tangible abdominal tumor is described. The computed tomography (CT) scan revealed a 7.5 cm, small intestine-associated tumor in the right abdomen. The patient received tumor resection and intestinal anastomosis. Further pathological examination confirmed the tumor as a duodenal fibromatosis that infiltrated the intestinal wall. To the best of our knowledge, this is the first report of a duodenum-derived fibromatosis that invaded the muscular layer of the intestine. Our study demonstrated that an unexplained abdominal mass should be noteworthy and properly treated.

  12. Histological structure of duodenum in gilts receiving low doses of zearalenone and deoxynivalenol in feed.

    Science.gov (United States)

    Lewczuk, Bogdan; Przybylska-Gornowicz, Barbara; Gajęcka, Magdalena; Targońska, Krystyna; Ziółkowska, Natalia; Prusik, Magdalena; Gajęcki, Maciej

    2016-01-01

    Deoxynivalenol (DON) and zearalenone (ZEN), produced by microfungi of the Fusarium family, are among the most commonly occurring mycotoxins. They are considered important factors affecting human and animal health as well as livestock productivity. The aim of this study was to determine the effect of low doses of these mycotoxins on the histological structure of the pig duodenum. The study was performed on 72 gilts, with initial weights of approximately 25kg, divided into 4 equal groups. Group I received per os ZEN (40μg/kg BW), group II-DON (12μg/kg BW), group III-ZEN (40μg/kg BW) and DON (12μg/kg BW), and group IV-vehicle. The pigs were killed after 1, 2, 3, 4, 5 and 6 weeks of the treatment, and the duodenum samples were prepared for histological investigations. The slides were digitalized and subjected to morphometrical analysis. The treatment with DON and ZEN did not change the architecture of the mucosa or the ratio between goblet and adsorptive cells in the epithelium. The administration of DON induced an increase in the number of lymphocytes in the mucosal epithelium. Both mycotoxins, administered alone or together, increased the quantity of lymphocytes, plasma cells and macrophages with black-brown granules in the lamina propria. The time-courses of changes in the number of defense system cells evoked by DON and ZEN were different. In conclusion, dietary exposure to low doses of Fusarium mycotoxins should be considered an important risk factor for subclinical inflammation in the small intestine. Copyright © 2015 Elsevier GmbH. All rights reserved.

  13. Diagnosis, treatment and prognosis of neuroendocrine tumor in stomach and duodenum

    Directory of Open Access Journals (Sweden)

    Xiang-yao WANG

    2016-04-01

    Full Text Available Objective  To investigate the clinicopathological characteristics and prognosis of patients with neuroendocrine tumor in stomach and duodenum for early diagnosis. Methods  The clinical, endoscopic and pathological data of 20 patients admitted to the PLA General Hospital from Jan. 2012 to Jan. 2015 and diagnosed as gastric and duodenal neuroendocrine tumor were collected for retrospective analysis. The histopathological classification of the disease was made according to the WHO 2010 Classification of the Neuroendocrine Neoplasms. Result  Ten male and 10 female patients aged between 35 and 77 (mean 55.5±10.6 years old were recruited in the present study. Tumor located in the stomach in 13 cases, and in duodenum in 7 cases. The maximum diameter of the tumor was 0.2-2.5cm. Endoscopic features included polypoid protrusion, hemispheric submucosal protrusion, and mucosal erosion. All the patients were treated endoscopically, among them, four patients were treated with electrocoagulation and electrosection, 10 by endoscopic resection (EMR, and 6 by endoscopic submucosal dissection (ESD. In one patient, surgical excision was done after ESD. Biopsy under gastroscopy and endoscopic ultrasonography were conducive to the diagnosis and treatment. According to the histopathological classification, 19 cases were classified as NET grade 1, and another one as NET grade 2. The follow-up study showed no metastasis and recurrence. Conclusions  The early diagnosis and treatment for gastric and duodenal neuroendocrine tumor can lead to satisfactory results. DOI: 10.11855/j.issn.0577-7402.2016.03.12

  14. Effect of caloric restriction on myenteric neuroplasticity in the rat duodenum during aging.

    Science.gov (United States)

    da Silva Porto, Gisele; Bertaglia Pereira, Joice Naiara; Tibúrcio, Vanessa Graciele; Stabille, Sandra Regina; Garcia de Faria, Haroldo; de Melo Germano, Ricardo; de Britto Mari, Renata

    2012-05-21

    The objective of this study was to evaluate the effects of caloric restriction (CR) on myenteric neurons in the duodenum of Wistar rats during aging. Thirty rats were divided into three groups: the C group (six-month-old animals that were fed a normal diet from weaning until six months of age), the SR group (18-month-old animals that were fed a normal diet from weaning until 18 months of age) and the CR group (18-month-old animals that were fed a 30% CR diet after six months of age). After 12 months, the animals were euthanized. Whole-mount preparations of the duodenums were either stained with Giemsa or underwent NADPH-diaphorase histochemistry to determine the general myenteric neuron population and the nitrergic neuron subpopulation (NADPH-d+), respectively. The NADPH-d-negative (NADPH-d-) neuron population was estimated based on the difference between the Giemsa-stained and NADPH-d+ neurons. The neurons were counted, and the cell body areas were measured. Aging was associated with neuronal loss in the SR group, which was minimized by caloric restriction in the CR group. The density (mm(2)) of the Giemsa-stained neurons was higher in the SR group (79.09 ± 6.25) than in the CR (92.37 ± 11.6) and C (111.68 ± 15.26) groups. The density of the NADPH-d+ neurons was higher in the SR group (44.90 ± 5.88) than in the C (35.75 ± 1.6) and RC (39.14 ± 7.02) groups. The density of NADPH-d- neurons was higher in the CR (49.73 ± 12.08) and C (75.64 ± 17.05) groups than in the SR group (33.82 ± 4.5). In the C group, 32% and 68% of the Giemsa-stained myenteric neurons were NADPH-d+ or NADPH-d-, respectively. With aging (SR group), the percentage of nitrergic neurons (56.77%) increased, whereas the percentage of NADPH-d- neurons (43.22%) decreased. In the CR group, the change in the percentage of nitrergic (42.37%) and NADPH-d- (57.62%) neurons was lower. As NADPH-d- neurons will be mostly cholinergic neurons, CR appears to reduce the loss of cholinergic neurons during

  15. CT appearance of the duodenum and mesenteric vessels in children with normal and abnormal bowel rotation

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, George A. [Harvard Medical School and Children' s Hospital Boston, Department of Radiology, Boston, MA (United States)

    2011-11-15

    Demonstration of the third duodenal segment (D3) in retroperitoneal location has been recently proposed as a method for excluding malrotation. This study was performed to determine whether a retroperitoneal third duodenal segment can reliably exclude malrotation. CTs of 38 patients with proven malrotation and 100 patients without malrotation were evaluated for the location of the duodenum/proximal small bowel, and the relationship of the superior mesenteric vein (SMV) to superior mesenteric artery (SMA). The D3 segment was in normal retroperitoneal location in 100% of control patients, compared to 2.5% or (1 of 38) of patients with malrotation. Nine of 11 patients (91%) with malrotation imaged prior to surgery had the proximal bowel in an abnormal location, while all 100 control patients had it in a normal location. The SMV was in normal relationship to the SMA in 11/38 patients (29%) with malrotation, compared to 79% of normal controls. In 10 controls, a branch of the SMV was partially wrapped around the SMA, potentially mimicking partial mesenteric volvulus. A retroperitoneal location of the D3 segment makes the diagnosis of malrotation unlikely but not impossible. Additional imaging of the duodenojejunal junction or cecum may be necessary to reliably exclude intestinal malrotation. (orig.)

  16. Suppression of calpain expression by NSAIDs is associated with inhibition of cell migration in rat duodenum.

    Science.gov (United States)

    Silver, Kristopher; Littlejohn, A; Thomas, Laurel; Bawa, Bhupinder; Lillich, James D

    2017-05-15

    Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used for the alleviation of pain and inflammation, but these drugs are also associated with a suite of negative side effects. Gastrointestinal (GI) toxicity is particularly concerning since it affects an estimated 70% of individuals taking NSAIDs routinely, and evidence suggests the majority of toxicity is occurring in the small intestine. Traditionally, NSAID-induced GI toxicity has been associated with indiscriminate inhibition of cyclooxygenase isoforms, but other mechanisms, including inhibition of cell migration, intestinal restitution, and wound healing, are likely to contribute to toxicity. Previous efforts demonstrated that treatment of cultured intestinal epithelial cells (IEC) with NSAIDs inhibits expression and activity of calpain proteases, but the effects of specific inhibition of calpain expression in vitro or the effects of NSAIDs on intestinal cell migration in vivo remain to be determined. Accordingly, we examined the effect of suppression of calpain protease expression with siRNA on cell migration in cultured IECs and evaluated the effects of NSAID treatment on epithelial cell migration and calpain protease expression in rat duodenum. Our results show that calpain siRNA inhibits protease expression and slows migration in cultured IECs. Additionally, NSAID treatment of rats slowed migration up the villus axis and suppressed calpain expression in duodenal epithelial cells. Our results are supportive of the hypothesis that suppression of calpain expression leading to slowing of cell migration is a potential mechanism through which NSAIDs cause GI toxicity. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Investigation of a role for reduction in ferric iron uptake by mouse duodenum.

    Science.gov (United States)

    Raja, K B; Simpson, R J; Peters, T J

    1992-06-10

    59Fe uptake rates by mouse duodenal fragments incubated in vitro were markedly reduced by non-permeable reagents, ferricyanide (oxidising agent) and ferrozine (Fe2+ chelator), in the medium; ferrocyanide had no effect. Reduction of Fe3+, as reflected by an increase in ferrozine-(Fe2+)-chelatable iron, was observed in the presence of the tissue fragments. The generation of Fe2+ occurred linearly with time, was independent of the medium ferrozine concentration, and was not due to release of reducing factors from the duodenal fragments. Fe(3+)-reducing activity was mainly present on the mucosal surface and was localised primarily to the proximal region of the small intestine. Changes in Fe3+ reduction rates closely parallelled the changes in duodenal 59Fe uptake, when metabolic inhibitors or modulators of membrane potential were included in the medium. The enhancement in duodenal mucosal 59Fe uptake in chronic hypoxic and iron-deficient mice parallelled the changes in the tissue reduction of medium Fe3+. Moreover, the rates of reduction were quantitatively similar to rates of uptake. These observations indicate that a sequential reduction and uptake process operates for Fe3+ uptake in mouse duodenum.

  18. Endoscopic and histopathological study on the duodenum of Strongyloides stercoralis hyperinfection

    Institute of Scientific and Technical Information of China (English)

    Kazuto Kishimoto; Akira Hokama; Tetsuo Hirata; Yasushi Ihama; Manabu Nakamoto; Nagisa Kinjo; Fukunori Kinjo; Jiro Fujita

    2008-01-01

    AIM: To investigate endoscopic and histopathological findings in the duodenum of patients with Strongyloides stercoralis (5. stercoralis) hyperinfection.METHODS: Over a period of 23 years (1984-2006), we investigated 25 patients with 5. stercoralis hyperinfection who had had an esophagogastroduodenoscopy before undergoing treatment for strongyloidiasis. The clinical and endoscopic findings were analyzed retrospectively.RESULTS: Twenty-four (96%) of the patients investigated were under immunocompromised condition which was mainly due to a human T lymphotropic virus type 1 (HTLV-1) infection. The abnormal endoscopic findings, mainly edematous mucosa, white villi and erythematous mucosa, were observed in 23 (92%) patients. The degree of duodenitis including villous atrophy/destruction and inflammatory cell infiltration corresponded to the severity of the endoscopic findings. The histopathologic yield for identifying larvae was 71.4% by duodenal biopsy. The endoscopic findings of duodenitis were more severe in patients whose biopsies were positive for larvae than those whose biopsies were negative (Endoscopic severity score: 4.86 ± 2.47 vs 2.71 ± 1.38, P < 0.05).CONCLUSION: Our study clearly demonstrates that, in addition to stool analysis, endoscopic observation and biopsies are very important. We also emphasize that 5. stercoralis and HTLV-1 infections should be ruled out before immunosuppressive therapy is administered in endemic regions.

  19. A Rare Case of an Intraductal Papillary Mucinous Neoplasm of Pancreas Fistulizing Into Duodenum With Adult Polycystic Kidney Disease

    Science.gov (United States)

    Pipaliya, Nirav; Rathi, Chetan; Parikh, Pathik; Patel, Ruchir; Ingle, Meghraj; Sawant, Prabha

    2015-01-01

    Intraductal papillary mucinous neoplasm (IPMN) accounts for 20-50% of all cystic neoplasms of the pancreas. Rarely, IPMN, whether benign or malignant, can fistulize into adjacent organs like duodenum, stomach or common bile duct. IPMN can be associated with other diseases like Peutz-Jeghers syndrome and familial adenomatous polyposis. Association with adult polycystic kidney disease (ADPKD) is extremely rare. We report a case of a 60-year-old male with a large IPMN in the head of the pancreas diagnosed by magnetic resonance imaging, endoscopic ultrasound and cyst fluid analysis. It was complicated by fistula formation into the second part of the duodenum. Patient was simultaneously having adult polycystic kidney disease. There is only one case report of uncomplicated IPMN with ADPKD in the literature so far. And even rarer, there is no any case report of fistulizing IPMN with ADPKD reported so far, to the best of our knowledge. PMID:27785296

  20. Intraductal Papillary Mucinous Neoplasm Occurring In Pancreatic Heterotopia Of The Duodenum: Two Cases And A Review Of The Literature

    Directory of Open Access Journals (Sweden)

    Sarah M Choi

    2008-09-01

    Full Text Available Context Heterotopic pancreas is usually an incidental finding during pathologic evaluation of gastrointestinal polyps or lesions encoun-tered during endoscopy for nonspecific symptoms or unrelated conditions. However, the same neoplastic processes that occur in normal pancreas also can occur in pancreatic heterotopias. Case report We report two cases of intraductal papillary mucinous neoplasms arising in pancreatic heterotopia within the duodenum of two patients. These cases are among the first reports of neoplasia occurring in pancre-atic heterotopia of the duodenum. Both patients are being managed expectantly, as there is currently no consensus regarding the proper follow up in these cases, particularly those that have been incompletely excised. Conclusion These cases highlight the potential for neo-plasia in pancreatic heterotopia and emphasize the importance of careful evaluation of these lesions. Close clinical follow up and possible excision may be warranted in patients with concerning pathologic or clinical findings.

  1. Duplication Cyst in the Third Part of the Duodenum Presenting with Gastric Outlet Obstruction and Severe Weight Loss

    Science.gov (United States)

    Shaheen, Osama; Sara, Samer; Safadi, Mhd Firas; Alsaid, Bayan

    2015-01-01

    Duodenal duplication is a rare developmental abnormality which is usually diagnosed in infancy and childhood, but less frequently in adulthood. We report a case of a 16-year-old female with a duplication cyst in the third part of the duodenum. The patient presented with symptoms of gastric outlet obstruction, including severe anorexia and weight loss. The diagnosis was made preoperatively by CT scan and upper endoscopy. The cyst was successfully treated by marsupialization on the duodenum using a GIA stapler. Duodenal duplication presents with a wide variety of symptoms. Although illusive, many cases can be properly diagnosed preoperatively by using the appropriate imaging modalities. Treatment choices are tailored according to the size and location of the cyst, in addition to its relation to adjacent structures. The outcomes are favorable in the majority of patients. PMID:26649220

  2. Role of superoxide dismutase enzymes and ascorbate in protection of nitrergic relaxation against superoxide anions in mouse duodenum

    Institute of Scientific and Technical Information of China (English)

    M Ata SECILMIS; Olcay Ergurhan KIROGLU; Nuran OGULENER

    2008-01-01

    Aim: The aim of this study was to investigate whether superoxide dismutase (SOD) enzymes and ascorbate play a role in the protection of the nitrergic relax-ation against superoxide anion inhibition in the mouse duodenum. Methods: The effects of exogenous SOD, N,N'-bis(salicylidene) ethylenediamine chlo-ride (EUK-8; a synthetic cell-permeable mimetic of the manganese SOD [Mn SOD] and ascorbate on relaxant responses induced by nitrergic nerve stimulation), exogenous nitric oxide (NO), and nitroglycerin were investigated in isolated mouse duodenum tissues. Results: Diethyidithiocarbamate (DETCA) inhibited the relaxation to exogenous NO and nitroglycerin, but not relaxation to electri-cal field stimulation (EFS). SOD and ascorbate partially prevented the inhibi-tory effect of DETCA on relaxation to NO, abut not to nitroglycerin. The DETCA-induced inhibition on nitroglycerin was prevented by ELrK-8. Hemoglobin, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazolinel-oxyl-3-oxide, and hydroxo-cobalamin inhibited the relaxation to NO, but not to EFS and nitroglycerin in the presence of DETCA. Pyrogallol and hydroquinone inhibited the relaxation to NO, but not to EFS and nitroglycerin. This inhibition was prevented by exog-enous SOD and ascorbate, but was not prevented by EUK-8. Pyrogallol and hy-droquinone did not inhibit the EFS-induced relaxation in the presence of DETCA. Duroquinone and 6-anilino-5.8-quinolinedione inhibited the relaxation to EFS, NO, and nitroglycerin, and this inhibition was prevented by EUK-8. Conclusion: These results suggest that the nitrergic neurotransmission in the mouse duode-num is protected by endogenous tissue antioxidants against superoxide anions, and Mn SOD, in addition to copper/zinc SOD, can protect NO from attack from superoxide anion generators intracellularly. Also, the possibility that the endog-enous neurotransmitter may not be the free NO but a NO-containing or NO-generating molecule in the mouse duodenum remains open.

  3. Intractable duodenal ulcer caused by transmural migration of gossypiboma into the duodenum - a case report and literature review

    Science.gov (United States)

    2014-01-01

    Background Gossypiboma is a term used to describe a mass that forms around a cotton sponge or abdominal compress accidentally left in a patient during surgery. Transmural migration of an intra-abdominal gossypiboma has been reported to occur in the digestive tract, bladder, vagina and diaphragm. Open surgery is the most common approach in the treatment of gossypiboma. However, gossypibomas can be extracted by endoscopy while migrating into the digestive tract. We report a case of intractable duodenal ulcer caused by transmural migration of gossypiboma successfully treated by duodenorrhaphy. A systemic literature review is provided and a scheme of the therapeutic approach is proposed. Case presentation A 61-year-old Han Chinese man presented with intermittent epigastric pain for the last 10 months. He had undergone laparoscopic cholecystectomy conversion to open cholecystectomy for acute gangrenous cholecystitis 10 months ago at another hospital. Transmural migration of gossypiboma into the duodenum was found. Endoscopic intervention failed to remove the entire gauze, and duodenal ulcer caused by the gauze persisted. Surgical intervention was performed and the gauze was removed successfully. The penetrated ulcer was repaired with duodenorrhaphy. The postoperative period was uneventful. We systematically reviewed the literature on transmural migration of gossypiboma into duodenum and present an overview of published cases. Our PubMed search yielded seven reports of transmural migration of retained surgical sponge into the duodenum. Surgical interventions were necessary in two patients. Conclusion Transmural migration of gossypiboma into the duodenum is a rare surgical complication. The treatment strategies include endoscopic extraction and surgical intervention. Prompt surgical intervention should be considered for emergent conditions such as active bleeding, gastrointestinal obstruction, or intra-abdominal sepsis. For non-emergent conditions, surgical intervention

  4. Histological and morphometrical indices of chicken - broilers duodenum by the influence of probiotics, used in different doses

    OpenAIRE

    KOTSIUMBAS G.I.; KOSTYNJUK A.K.; SHCHEBENTOVSKA O.M.

    2012-01-01

    This work deals with histostructure and histomorphometrical indices of hair length, crypt depth and muscle layer thickness, the wall of chickens-broilers duodenum by the influence of different doses of probiotics. If was determined that using probiotics “Probion’’ and “Вio’’ in different doses favour veritable rising of hair length, the thickness of muscle layer wall, that indicates the active process of digestion, absorbion and improvement and normalization of poultry intestine tube digestio...

  5. Histological and morphometrical indices of chicken - broilers duodenum by the influence of probiotics, used in different doses

    OpenAIRE

    KOTSIUMBAS G.I.; KOSTYNJUK A.K.; SHCHEBENTOVSKA O.M.

    2012-01-01

    This work deals with histostructure and histomorphometrical indices of hair length, crypt depth and muscle layer thickness, the wall of chickens-broilers duodenum by the influence of different doses of probiotics. If was determined that using probiotics “Probion’’ and “Вio’’ in different doses favour veritable rising of hair length, the thickness of muscle layer wall, that indicates the active process of digestion, absorbion and improvement and normalization of poultry intestine tube digestio...

  6. Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats

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    Jacqueline Nelisis Zanoni

    2011-03-01

    Full Text Available CONTEXT: Peripheral neuropathy is one of the chronic complications of diabetes mellitus and is directly related to gastrointestinal consequences of the disease. Myenteric neurons are affected in some pathological conditions such as diabetic neuropathy. The imbalance between cellular antioxidants and free radicals, leading to an increase in oxidative stress, is considered one of the main factors responsible for neuronal damages in diabetes. Drugs that reduce the oxidative stress may play a significant role in the treatment of neurological complications of diabetes mellitus. OBJECTIVE: To evaluate the effect of L-glutamine supplementation on the myenteric neurons from the cecum and duodenum of Wistar rats with streptozotocin-induced diabetes mellitus. METHODS: The animals were divided in four groups (n = 5: non-treated normoglycemics, normoglycemics treated with L-glutamine, non-treated diabetics and diabetics treated with L-glutamine from the 4th day of diabetes induction on. The amino acid L-glutamine was added to their diet at 1%. Giemsa's technique was employed to stain the myenteric neurons. We determined the cell body area of 500 neurons in each group studied. The quantitative analysis was performed by sampling in an area of 16.6 mm² in the cecum and 3.6 mm² in the duodenum of each animal. RESULTS: After the supplementation with L-glutamine in the duodenum, we observed a preservation of neuronal density in groups normoglycemic and diabetic (P<0.05. We also observed a preservation of the cell bodies area in diabetic animals (group treated with L-glutamine (P<0.05. In the cecum, that preservation was not evident. CONCLUSION: Supplementation with L-glutamine (1% promoted a neuroprotective effect on the myenteric neurons from the duodenum of rats, both in terms of natural aging and of diabetes mellitus.

  7. Primary small-cell neuroendocrine carcinoma of the duodenum – a case report and review of literature

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    Nagai Hideo

    2004-08-01

    Full Text Available Abstract Background Small-cell neuroendocrine carcinoma in the duodenum is an extremely rare neoplasm with poor prognosis. Case presentation A 57-year-old man presented with sudden onset gastrointestinal bleeding and fainting attacks. Duodenoscopy and hypotonic duodenography revealed a 3 × 3 cm protruding tumor with ulcerations situated opposite the ampulla of Vater in the second part of the duodenum. Local excision of the tumor was performed, followed by adjuvant chemotherapy with 5-fluoro uracil and leucovorin. Examination of the tumor by immunohistochemistry and electron microscopy indicated it to be neuroendocrine in nature, expressing synaptophysin and AE1/AE3, and containing dense core granules. The patient showed no sign of recurrence and has been disease-free for more than 48 months after surgery. Conclusions Most cases of small-cell neuroendocrine carcinoma in the duodenum show rapid progression of the disease, and even radical surgery with or without chemotherapy do not prevent death. We report a rare subtype of small-cell neuroendocrine carcinoma. This subtype appears to have a much better prognosis, and may be amenable to local excision, if the lesion is away from the ampulla of Vater.

  8. Mechanism for enhanced absorption of a solid dispersion formulation of LY2300559 using the artificial stomach duodenum model.

    Science.gov (United States)

    Polster, Christopher S; Wu, Sy-Juen; Gueorguieva, Ivelina; Sperry, David C

    2015-04-06

    An artificial stomach duodenum (ASD) model has been used to demonstrate the performance difference between two formulations of LY2300559, a low-solubility acidic developmental drug. The two formulations investigated were a conventional high-shear wet granulation (HSWG) formulation and a solid dispersion formulation. A pharmacokinetic study in humans demonstrated the enhanced performance of the solid dispersion formulation relative to the HSWG formulation. The Cmax and AUC of the solid dispersion was 2.6 and 1.9 times greater, respectively, compared to the HSWG formulation. In the ASD, the solid dispersion formulation performance was characterized by three main phases: (1) rapid release in the stomach, creating a supersaturated concentration of drug, (2) precipitation in the stomach, and (3) rapid redissolution of the precipitate in the duodenum to concentration levels that are supersaturated relative to crystalline drug. A series of complementary experiments were employed to describe this performance behavior mechanistically. Imaging experiments with a pH indicating dye showed that local pH gradients from meglumine in the solid dispersion formulation were responsible for creating a high initial supersaturation concentration in the stomach. Upon dissipation of meglumine, the drug precipitated in the stomach as an amorphous solid. Because the precipitated drug is in an amorphous form, it can then rapidly redissolve as it transits to the more neutral environment of the duodenum. This unexpected sequence of physical state changes gives a mechanistic explanation for the enhanced in vivo performance of the solid dispersion formulation relative to the HSWG formulation.

  9. Assessment of the mutagenic potential of hexavalent chromium in the duodenum of big blue® rats.

    Science.gov (United States)

    Thompson, Chad M; Young, Robert R; Dinesdurage, Harshini; Suh, Mina; Harris, Mark A; Rohr, Annette C; Proctor, Deborah M

    2017-09-01

    A cancer bioassay on hexavalent chromium Cr(VI) in drinking water reported increased incidences of duodenal tumors in B6C3F1 mice at exposures of 30-180ppm, and oral cavity tumors in F344 rats at 180ppm. A subsequent transgenic rodent (TGR) in vivo mutation assay in Big Blue® TgF344 rats found that exposure to 180ppm Cr(VI) in drinking water for 28days did not increase cII transgene mutant frequency (MF) in the oral cavity (Thompson et al., 2015). Herein, we extend our analysis to the duodenum of these same TgF344 rats. At study termination, duodenum chromium levels were below either the limit of detection or quantification in control rats, but were 24.6±3.8μg/g in Cr(VI)-treated rats. The MF in control (23.2×10(-6)) and Cr(VI)-treated rats (22.7×10(-6)) were nearly identical. In contrast, the MF in the duodenum of rats exposed to 1-ethyl-1-nitrosourea for six days (study days 1, 2, 3, 12, 19, 26) increased 24-fold to 557×10(-6). These findings indicate that mutagenicity is unlikely an early initiating event in Cr(VI)-induced intestinal carcinogenesis. Copyright © 2017 Elsevier Inc. All rights reserved.

  10. Helicobacter species ribosomal DNA in the pancreas, stomach and duodenum of pancreatic cancer patients

    Institute of Scientific and Technical Information of China (English)

    Hans-Olof Nilsson; Unne Stenram; Ingemar Ihse; Torkel Wadstr(o)m

    2006-01-01

    AIM: To determine whether gastric and enteric Helico-bacter species are associated with pancreatic cancer.METHODS: Patients with exocrine pancreatic cancer(n = 40), neuroendocrine cancer (n = 14), multipleendocrine neoplasia type 1 (n = 8), and chronic pan-creatitis (n = 5) were studied. Other benign pancreat-ic diseases (n = 10) and specimens of normal pancre-as (n = 7) were included as controls. Pancreatic tissuespecimens were analyzed by Helicobacter-specificPCR-assay and products were characterized by dena-turing gradient electrophoresis and DNA-sequencing.From a subset of the pancreatic cancer patients, gas-tric and/or duodenal tissue as well as gallbladder andductus choledochus tissue were analyzed. Gallbladderand choledochus samples were included as controls.Stomach and duodenum samples were investigated toanalyze whether a gastric helicobacter might dissemi-nate to the pancreas in pancreatic cancer patients.Pancreatic specimens were analyzed by Bacteroides-specific PCR for detecting the translocation of indig-enous gut microbes to the diseased pancreas.RESULTS: Helicobacter DNA was detected in pancreas(tumor and/or surrounding tissue) of 75% of patientswith exocrine cancer, 57% of patients with neuro-endocrine cancer, 38% of patients with multiple eh-docrine neoplasia, and 60% of patients with chronicpancreatitis. All samples from other benign pancreaticdiseases and normal pancreas were negative. Thirty-three percent of the patients were helicobacter-positive in gastroduodenal specimens. Surprisingly,H. bilis was identified in 60% of the positive gastro-duodenal samples. All gallbladder and ductus cho-ledochus specimens were negative for helicobacter.Bacteroides PCR-assay was negative for all pancreaticsamples.CONCLUSION: Helicobacter DNA commonly detectedin pancreatic cancer suggests a possible role of theemerging pathogens in the development of chronicpancreatitis and pancreatic cancer.

  11. Periampullary mass--a rare presentation of poorly differentiated neuroendocrine cancer of duodenum in a young adult: a case report and review of literature.

    Science.gov (United States)

    Singh, Neha; Nayak, Hemanta K; Bagchi, Avishek; Kar, Premashis

    2012-10-09

    Poorly differentiated neuroendocrine tumour in the periampullary region of the duodenum is a rare entity. This entity usually present in old men. Here we report a periampullary poorly differentiated neuroendocrine cancer (PDEC) of duodenum presenting in a young man with subacute history of jaundice, abdominal pain, pancreatitis and constitutional symptoms. MRI localised the tumour and endoscopy-guided biopsy of the lesion proved the diagnosis. Although palliative surgery and chemotherapy were planned, the patient opted to leave against medical advice.

  12. Myenteric neuronal plasticity induced by Toxoplasma gondii (genotype III on the duodenum of rats

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    Rodrigo M. Papazian-Cabanas

    2012-09-01

    Full Text Available The effects of acute and chronic infection caused by Toxoplasma gondii on duodenal myenteric neurons were analyzed. Eighteen rats were assigned into four groups: Acute Control Group (ACG, n=4; Acute Experimental Group (AEG, n=4; Chronic Control Group (CCG, n=5; and Chronic Experimental Group (CEG, n=5. Rats from the AEG and CEG were inoculated orally with 105 genotype III (BTU-II strain tachyzoites of T. gondii isolated from a dog with neurological signs. Acute groups were killed after 24 hours after the inoculation and the chronic groups after 30 days. Whole-mount from the duodenum were stained with Giemsa. The population density of myenteric neurons, as well the body cell, nuclear and cytoplasmic area were analyzed. Both acute and chronic toxoplasmic infection did not provoke neuronal loss. On the other hand, plastic alterations were observed: decreasing of the nuclear and cytoplasmic area during the acute phase and neuronal hypertrophy during the chronic phase.Foram analisados os efeitos da infecção aguda e crônica provocada pelo Toxoplasma gondii sobre os neurônios mientéricos do duodeno. Dezoito ratos foram divididos em quatro grupos: Grupo Controle Agudo (GCA, n= 4, Grupo Experimental Agudo (GEA, n=4, Grupo Controle Crônico (GCC, n=5 e Grupo Experimental Crônico (GEC, n=5. Os animais do GEA e GEC receberam por via oral 10 5 taquizoítos de Toxoplasma gondii da cepa BTUII (genótipo III isolada de um cão com sintomatologia neurológica. Os grupos agudos foram submetidos à eutanásia após 24 horas e os crônicos após 30 dias da inoculação. Preparados totais do duodeno foram corados com Giemsa. A densidade populacional dos neurônios mientéricos, bem como a área do corpo celular, núcleo e citoplasma foram analisados. Ambas, as infecções toxoplásmicas aguda e crônica não provocaram a perda neuronal. Por outro lado, alterações plásticas foram observadas: diminuição da área nuclear e citoplasmática durante a fase

  13. Expression of iron-related proteins in the duodenum is up-regulated in patients with chronic inflammatory disorders

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    Molly Jacob

    2015-01-01

    Full Text Available Mechanisms responsible for derangements in iron homeostasis in chronic inflammatory conditions are not entirely clear. The aim of this study was to test the hypothesis that inflammation affects expression of iron-related proteins in the duodenum and monocytes in patients with chronic inflammatory disorders, thus contributing to dysregulated iron homeostasis. Duodenal mucosal samples and peripheral blood monocytes obtained from patients with chronic inflammatory disorders, viz. ulcerative colitis (UC, Crohn’s disease (CD and rheumatoid arthritis (RA, were used for gene and protein expression studies. Haemoglobin levels were significantly lower and serum C-reactive protein (CRP levels significantly higher in those in the disease groups. Gene expression of several iron-related proteins in the duodenum was significantly up-regulated in patients with UC and CD. In those with UC, it was found that protein expression of divalent metal transporter (DMT1 and ferroportin, which are involved in absorption of dietary non-heme iron, was also significantly higher in the duodenal mucosa. Gene expression of the duodenal proteins of interest correlated positively with one another and negatively with haemoglobin. Gene expression of iron-related proteins in monocytes was studied in patients with UC and found to be unaffected. In a separate group of patients with UC, serum hepcidin levels were found to be significantly lower than in control subjects. In conclusion, expression of iron related proteins was up-regulated in the duodenum of patients with chronic inflammatory conditions in this study. The effects appeared to be secondary to anemia and the consequent erythropoietic drive.

  14. Right hemicolectomy combined with pancreatico-duodenectomy for the treatment of colon carcinoma invading the duodenum or pancreas

    Institute of Scientific and Technical Information of China (English)

    SONG Xin-ming; LAN Ping; WANG Lei; ZHAN Wen-hua; WANG Jian-ping; HE Yu-long; LIAN Lei; CAI Guan-fu; ZHOU Hong-feng; HUANG Bao-yu

    2006-01-01

    @@ Locally advanced carcinoma in sigmoid colon and rectum often invades adjacent organs in the pelvis; whereas local invasion of the pancreas or duodenum is rare. Consequently combined hemicolectomy and pancreatico-duodenectomy in the treatment of locally advanced colorectal carcinoma is uncommon.1 To the best of our knowledge, there are currently only three such cases reported in the Chinese literature to date.2 This paper reports five cases of synchronous right hemicolectomy (RH) and pancreatico-duodenectomy(PD) for the treatment of locally advanced right colon carcinoma.

  15. Postruminal synthesis modifies the odd- and branched-chain fatty acid profile from the duodenum to milk.

    Science.gov (United States)

    Vlaeminck, B; Gervais, R; Rahman, M M; Gadeyne, F; Gorniak, M; Doreau, M; Fievez, V

    2015-07-01

    Milk odd- and branched-chain fatty acids (OBCFA) have been suggested as potential biomarkers for rumen function. The potential of milk OBCFA as a biomarker depends on whether their profile reflects the profile observed in the duodenum. The objective of this study was to evaluate whether the OBCFA profile in duodenum samples is reflected in plasma and milk. For this, 2 dairy cattle experiments were used. In experiment 1, 4 Holstein cows fitted with rumen and proximal duodenum cannulas were used in a 4×4 Latin square design. The treatments consisted of 2 nitrogen levels (143 vs. 110g of crude protein/kg of dry matter for high and low N, respectively) combined with either 1 of the 2 energy sources (i.e., starch from barley, corn, and wheat or fiber from soybean hulls and dehydrated beet pulp). In experiment 2, 4 Holstein cows fitted with rumen and proximal duodenum cannulas were used in a 3×3 Latin square design, with the treatments consisting of 3 diets: (1) RNB-, a diet with a crude protein content of 122g/kg of dry matter, predicted to provide protein digested in the small intestine according to the requirement of the animals, but with a shortage of rumen degradable protein; (2) RNB- to which 6g/d of niacin was added through inclusion in the mineral and vitamin premix, and (3) RNB- to which urea was added to balance rumen degradable N supply resulting in a CP content of 156g/kg of dry matter. In both experiments, samples of duodenal digesta, plasma, and milk were collected and analyzed for fatty acids. Additionally, lipids in plasma samples were separated in lipid classes and analyzed for fatty acids. The OBCFA profile in milk was enriched in 15:0, iso-17:0, anteiso-17:0, and cis-9-17:1 as compared with duodenal samples, and milk secretions even exceeded duodenal flows, which suggests occurrence of postruminal synthesis, such as de novo synthesis, desaturation, and elongation. The postruminal modification of the OBCFA profile might hamper the application of OBCFA

  16. The effect of free and protected oils on the digestion of dietary carbohydrates between the mouth and duodenum of sheep.

    Science.gov (United States)

    McAllan, A B; Knight, R; Sutton, J D

    1983-05-01

    Sheep fitted with rumen and re-entrant duodenal cannulas were given diets of approximately 200 g hay and 400 g concentrate mixture alone, or supplemented daily with 40 g linseed or coconut oils free or protected with formaldehyde-casein in a 5 x 5 Latin-square arrangement. Chromic oxide paper was given as a marker at feeding time and passage to the duodenum of neutral-detergent fibre (NDF) and different sugars were estimated from the values for constituent:marker at the duodenum. Contributions of microbial carbohydrates to these flows were estimated from amounts of RNA present. The carbohydrate composition of mixed rumen bacteria from sheep rumen digesta were similar regardless of diet. Of the sugars entering the duodenum all the rhamnose and ribose and 0.51, 0.24 and 0.35 of the mannose, galactose and starch-glucose respectively, were contributed by the microbes. Virtually all the arabinose, xylose and cellulose-glucose were contributed by the diet. For sheep receiving the basal ration, coefficients of digestibility between mouth and duodenum, corrected where necessary for microbial contribution, were 0.95, 0.66, 0.67, 0.62, 0.45 and 0.51 for starch-glucose, mannose, arabinose, galactose, xylose and cellulose-glucose respectively. Corresponding values when free-oil-supplemented diets were given were 0.95, 0.55, 0.38, 0.55, 0.01 and -0.02 respectively. Values for diets supplemented with linseed oil or coconut oil did not differ significantly. Addition of protected oils to the basal feed also resulted in depressed digestibilities of dietary structural sugars but to a far lesser extent than those observed with the free oils. Apparent digestibility of NDF was altered in the same direction as those of the main structural sugars, averaging 0.50, 0.17 and 0.29 in animals receiving the basal, free-oil-supplemented or protected-oil-supplemented diets respectively. The reasons for the difference between NDF and discrete carbohydrate analytical totals are discussed.

  17. Application of an Artificial Stomach-Duodenum Reduced Gastric pH Dog Model for Formulation Principle Assessment and Mechanistic Performance Understanding.

    Science.gov (United States)

    Lee, Chen-Ming; Luner, Paul E; Locke, Karen; Briggs, Katherine

    2017-02-23

    The objective of this study was to develop an artificial stomach-duodenum (ASD) dissolution model as an in vitro evaluation tool that would simulate the gastrointestinal physiology of gastric pH-reduced dogs as a method to assess formulations for a poorly soluble free acid compound with ng/mL solubility. After establishing the ASD model with well controlled duodenum pH, five formulations each applying different solubilization principles were developed and their performance in the ASD model and in vivo in dogs was evaluated. Excellent correlations were obtained between dog AUC and ASD AUC of five formulations evaluated with SIF (r(2) = 0.987) and FaSSIF (r(2) = 0.989) as the duodenum dissolution medium, indicating that the approach of infusing NaOH into duodenum compartment to maintain duodenum pH of an ASD worked properly in simulating gastric pH-reduced dog. Raman spectroscopy was used to study drug dissolution kinetics associated with different solubilization principles and the results suggested that the solubilization principles performed as designed. Spectroscopic results also identified that the compound formed a gel during dissolution and HPMC maintained the drug gelled state to avoid further solid form conversion. The implication of the compound physical gelation to drug dissolution kinetics and in vivo exposure are discussed.

  18. A prospective study of differences in duodenum compared to remaining small bowel motion between radiation treatments: Implications for radiation dose escalation in carcinoma of the pancreas

    Directory of Open Access Journals (Sweden)

    Wahab Sasha H

    2006-09-01

    Full Text Available Abstract Purpose As a foundation for a dose escalation trial, we sought to characterize duodenal and non-duodenal small bowel organ motion between fractions of pancreatic radiation therapy. Patients and methods Nine patients (4 women, 5 men undergoing radiation therapy were enrolled in this prospective study. The patients had up to four weekly CT scans performed during their course of radiation therapy. Pancreas, duodenum and non-duodenal small bowel were then contoured for each CT scan. On the initial scan, a four-field plan was generated to fully cover the pancreas. This plan was registered to each subsequent CT scan. Dose-volume histogram (DVH analyses were performed for the duodenum, non-duodenal small bowel, large bowel, and pancreas. Results With significant individual variation, the volume of duodenum receiving at least 80% of the prescribed dose was consistently greater than the remaining small bowel. In the patient with the largest inter-fraction variation, the fractional volume of non-duodenal small bowel irradiated to at least the 80% isodose line ranged from 1% to 20%. In the patient with the largest inter-fraction variation, the fractional volume of duodenum irradiated to at least the 80% isodose line ranged from 30% to 100%. Conclusion The volume of small bowel irradiated during four-field pancreatic radiation therapy changes substantially between fractions. This suggests dose escalation may be possible. However, dose limits to the duodenum should be stricter than for other segments of small bowel.

  19. Evaluation of the areas of neuronal cell bodies and nuclei in the myenteric plexus of the duodenum of adult rats

    Directory of Open Access Journals (Sweden)

    MIRANDA-NETO MARCILÍO H.

    2000-01-01

    Full Text Available This study compared the areas of cell body and nucleus profiles of the myenteric neurons in the antimesenteric and intermediate regions of the duodenum of adult rats. Five male rats were used. The duodenum was removed and dissected to whole-mount preparations, which were stained by the Giemsa technique. The areas of cell body and nucleus profiles of 100 neurons, 50 from each region, of each animal, were assessed with image analyser. Based on the global mean±SD of the areas of cell body profiles, neurons were labelled as small, medium or large. It was observed that the neurons did not differ significantly in size or incidence between the antimesenteric and intermediate regions. However, the nuclei of the small and medium neurons were significantly smaller in the latter region. It is discussed that the smaller nuclear size could be related to the cell bodies being slightly smaller on this region and to a possible smaller biosynthetic activity which would influence nuclear size.

  20. Optimizing the villi visualization by tethered capsule OCT endomicroscopy for comprehensive imaging of human duodenum (Conference Presentation)

    Science.gov (United States)

    Dong, Jing; Gora, Michalina J.; Beaulieu-Ouellet, Emilie; Queneherve, Lucille H.; Grant, Catriona N.; Rosenberg, Mireille; Nishioka, Norman S.; Fasano, Alessio; Tearney, Guillermo J.

    2017-02-01

    Celiac disease (CD) affects around 1% of the global population and can cause serious long-term symptoms including malnutrition, fatigue, and diarrhea, amongst others. Despite this, it is often left undiagnosed. Currently, a tissue diagnosis of CD is made by random endoscopic biopsy of the duodenum to confirm the existence of microscopic morphologic alterations in the intestinal mucosa. However, duodenal endoscopic biopsy is problematic because the morphological changes can be focal and endoscopic biopsy is plagued by sampling error. Additionally, tissue artifacts can also an issue because cuts in the transverse plane can make duodenal villi appear artifactually shortened and can bias the assessment of intraepithelial inflammation. Moreover, endoscopic biopsy is costly and poorly tolerated as the patient needs to be sedated to perform the procedure. Our lab has previously developed technology termed tethered capsule OCT endomicroscopy (TCE) to overcome these diagnostic limitations of endoscopy. TCE involves swallowing an optomechanically-engineered pill that generates 3D images of the GI tract as it traverses the lumen of the organ via peristalsis, assisted by gravity. In several patients we have demonstrated TCE imaging of duodenal villi, however the current TCE device design is not optimal for CD diagnosis as the villi compress when in contact with the smooth capsule's wall. In this work, we present methods for structuring the outer surface of the capsule to improve the visualization of the villi height and crypt depth. Preliminary results in humans suggest that new TCE capsule enables better visualization of villous architecture, making it possibly to comprehensively scan the entire duodenum to obtain a more accurate tissue diagnosis of CD.

  1. Role of the duodenum in regulation of plasma ghrelin levels and body mass index after subtotal gastrectomy

    Institute of Scientific and Technical Information of China (English)

    Hai-Tao Wang; Qi-Cheng Lu; Qing Wang; Rong-Chao Wang; Yun Zhang; Hai-Long Chen; Hong Zhao; Hai-Xin Qian

    2008-01-01

    AIM: To investigate the role of the duodenum in the regulation of plasma ghrelin levels and body mass index (BMI), and the correlation between them after subtotal gastrectomy.METHODS: Forty-two patients with To-1No-1M0 gastric cancer were divided into two groups after gastrectomy according to digestive reconstruction pattern,Billroth I group (n = 23) and Billroth Ⅱ group (n = 19).Ghrelin levels were determined with radioimmunoassay (RIA) before and on d 1, 7, 30 and 360 after gastrectomy,and BMI was also measured.RESULTS: The two groups had identical postoperative trends in ghrelin alterations during the early stage, both decreasing sharply to a nadir on d 1 (36.7% vs 35.7%),then markedly increasing on d 7 (51.0% vs 51.1%). On d 30, ghrelin levels in the Billroth I group were slightly higher than those in the Billroth Ⅱ group. However,those of the Billroth I group recovered to 93.6% on d 360, which approached, although lower than, the preoperative levels, and no statistically significant difference was observed. Those of the Billroth Ⅱ group recovered to only 81.6% and manifested significant discrepancy with preoperative levels (P = 0.033).Compared with preoperative levels, ghrelin levels of the two groups decreased by 6.9% and 18.4% and BMI fell by 3.3% and 6.4%, respectively. The linear regression correlations were revealed in both groups between decrease of ghrelin level and BMI (R12 = 0.297, P = 0.007;R22 = 0.559, P < 0.001).CONCLUSION: Anatomically and physiologically, the duodenum compensatively promotes ghrelin recovery and accordingly enhances BMI after gastrectomy.Regarding patients with insufficient ghrelin secretion,ghrelin is positively associated with BMI.

  2. Cholecystokinin but not ghrelin stimulates mucosal bicarbonate secretion in rat duodenum: independence of feeding status and cholinergic stimuli.

    Science.gov (United States)

    Sjöblom, Markus; Lindqvist, Ramin; Bengtsson, Magnus W; Jedstedt, Gunilla; Flemström, Gunnar

    2013-05-10

    Cholecystokinin (CCK) is an important regulator of food digestion but its influence on small intestinal secretion has received little attention. We characterized effects of CCK-8, ghrelin and some related peptides on duodenal HCO3(-) secretion in vivo and demonstrated CCK-induced calcium signaling in acutely isolated enterocytes. A segment of proximal duodenum with intact blood supply was cannulated in situ in anaesthetized rats. Mucosal HCO3(-) secretion was continuously recorded (pH-stat). Peptides were administrated to the duodenum by close intra-arterial infusion. Clusters of duodenal enterocytes were attached to the bottom of a perfusion chamber. The intracellular calcium concentration ([Ca(2+)]i) was examined by dual-wavelength imaging. CCK-8 (3.0, 15 and 60 pmol/kg,h) caused dose-dependent increases (psecretion in both overnight fasted and continuously fed animals. The CCK1R-antagonist devazepide but neither the CCK2R-antagonist YMM022 nor the melatonin MT2-selective antagonist luzindole inhibited the rise in secretion. Atropine decreased sensitivity to CCK-8. The appetite-related peptide ghrelin was without effect on the duodenal secretion in fasted as well as fed animals. Superfusion with CCK-8 (1.0-50 nM) induced [Ca(2+)]i signaling in acutely isolated duodenal enterocytes. After an initial peak response, [Ca(2+)]i returned to near basal values within 3-5min. Devazepide but not YMM022 inhibited this [Ca(2+)]i response. Low doses of CCK-8 stimulate duodenal alkaline secretion and induce enterocyte [Ca(2+)]i signaling by an action at CCK1 receptors. The results point to importance of CCK in the rapid postprandial rise in mucosa-protective duodenal secretion.

  3. Burkitt's lymphoma of the duodenum in a patient with AIDS Linfoma de Burkitt do duodeno em um paciente com AIDS

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    Marcelo Corti

    2007-06-01

    Full Text Available Non-Hodgkin's lymphoma of B-cell type is the second most common neoplasm after Kaposi's sarcoma, among patients with human immunodeficiency virus infection. Most non-Hodgkin's lymphoma cases that are associated with acquired immunodeficiency syndrome involve extranodal sites, especially the digestive tract and the central nervous system. We report a case of primary lymphoma of the duodenum in a patient with AIDS. Upper gastrointestinal endoscopy revealed pseudopolypoid masses found in the second portion of the duodenum. A complete diagnostic study including histological, immunohistochemical and virological analyses showed high-grade B-cell Burkitt's lymphoma. The Epstein-Barr virus genome was detected in biopsies by immunohistochemical and in situ hybridization.O linfoma não-Hodgkin de células B é a segunda neoplasia mais comum em pacientes com infecção pelo vírus da imunodeficiência humana depois do sarcoma de Kaposi. A maioria dos casos de linfoma não-Hodgkin associados com a síndrome da imunodeficiência adquirida envolve locais extraganglionares, especialmente o trato digestivo e o sistema nervoso central. Nós relatamos um caso de linfoma primário do duodeno em um paciente com AIDS. Uma endoscopia digestiva alta mostrou massas pseudopolipóides encontradas na segunda porção do duodeno. Um estudo diagnóstico completo incluindo exames histológicos, imunohistoquímicos e virológicos mostrou um linfoma de células B tipo Burkitt. Detectou-se genoma do vírus Epstein-Barr em biópsias por hibridização in situ e imuno-histoquímica.

  4. Fluid mechanical consequences of pendular activity, segmentation and pyloric outflow in the proximal duodenum of the rat and the guinea pig.

    Science.gov (United States)

    de Loubens, Clément; Lentle, Roger G; Love, Richard J; Hulls, Corrin; Janssen, Patrick W M

    2013-06-06

    We conducted numerical experiments to study the influence of non-propagating longitudinal and circular contractions, i.e. pendular activity and segmentation, respectively, on flow and mixing in the proximal duodenum. A lattice-Boltzmann numerical method was developed to simulate the fluid mechanical consequences for each of 22 randomly selected sequences of high-definition video of real longitudinal and radial contractile activity in the isolated proximal duodenum of the rat and guinea pig. During pendular activity in the rat duodenum, the flow was characterized by regions of high shear rate. Mixing was so governed by shearing deformation of the fluid that increased the interface between adjacent domains and accelerated their inter-diffusion (for diffusion coefficients approx. less than 10(-8) m² s(-1)). When pendular activity was associated with a slow gastric outflow characteristic of post-prandial period, the dispersion was also improved, especially near the walls. Mixing was not promoted by isolated segmentative contractions in the guinea pig duodenum and not notably influenced by pylorus outflow. We concluded that pendular activity generates mixing of viscous fluids 'in situ' and accelerates the diffusive mass transfer, whereas segmentation may be more important in mixing particulate suspensions with high solid volume ratios.

  5. Histological and Histomorphometric studies of ethanol-injured pylorus and duodenum of Wistar rats pre-treated with Moringa oliefera extract

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    Olayemi Kafilat Olaibi

    2014-04-01

    Full Text Available Background: This study investigated the effects of pre-treatment with Moringa oleifera extract on the histomorphology and histomorphometry of the pylorus and duodenum of rats following ethanol-induced gastrointestinal injury. Methods: Following extract and ethanol treatment, pylorus and duodenum of the rats were processed for histological procedures, and tissue quantification of total antioxidant capacity, to access the protective abilities of the extract. Results: We showed that Moringa oleifera extract significantly reduces (p<0.05 total lesion area of the secretory portion of stomach following ethanol induced damage. Also the extract, to a great extent preserved the histological integrity of the pylorus and duodenum, and also significantly improved (p<0.05 the % of glandular mucosa of the pylorus that was intact and the ratio of villi height to crypt depth following ethanol damage. There was significant difference in pyloric and duodenal concentration of total antioxidant capacity between ethanol injured rats compared to control and extract treated groups. Conclusion: The present study confirms the antiulcer properties of Moringa oleifera extract, and demonstrates that the extract preserves the histological integrity and dimensions of the various layers of pylorus and duodenum of Wistar rats, thereby preventing a decrease gastrointestinal surface area following ethanol induced injury.

  6. Phospholipid Profile of the Stomach and Duodenum of Normal Rabbits Fed with Supplements of Unripe Pawpaw (Carica papaya) and Unripe Plantain (Musa sapientum) Extract

    Science.gov (United States)

    Eriyamremu, G. E.; Asagba, S. O.; Osagie, V. E.; Ojeaburu, S. I.; Lolodi, O.

    This study reports the effect of unripe pawpaw and/or unripe plantain extracts on the phospholipid profile of the stomach and duodenum of normal rabbits. Supplementation of chow with unripe pawpaw or plantain extract significantly reduced (p< 0.05) the weight gained by rabbits compared with the control. The total phospholipids content in the stomach and duodenum were significantly increased (p< 0.05) in the test groups compared to the control. In the stomach and duodenum, phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SGM) were significantly increased (p< 0.05) in the test groups compared to the control. Pawpaw occasioned a decrease in the PE/PC ratio in both tissues as against an increase observed in the SGM/PC ratio. The results presented suggests that unripe pawpaw meal and unripe plantain extract alter the phospholipid profile of the stomach and duodenum in ways which may affect membrane fluidity of these tissues and would have profound effect on the gastro-duodenal mucosa and thus have implication(s) for gastric and duodenal ulcers in rabbits.

  7. Studies on changes in bulks of body per dose and in the positioning of duodenum by respiration when treating pancreatic cancer patients with radiation therapy

    Energy Technology Data Exchange (ETDEWEB)

    Jang, Hyeong Jun; Chun, Geum Seong; Park, Yeong Gyu [Dept. of Radiation Oncology, Seoul St Mary' s Hospital, Seoul (Korea, Republic of)

    2014-06-15

    In the case of treating pancreatic cancer, the importance is put on the spread of dose. Changes take place in duodenum in accordance with respiration. Thus, in this paper, I am going to trace the positioning of duodenum and the changes in bulks of body per dose by scanning the patients' Kilo voltage Cone-Beam CT using the hospital equipped CT-on rail System. Seeing three patients, I have acquired KVCBCT by using CT-on rail System and spotted the change in positioning at duodenum after comparing with the preliminary image of treatment plan by using SYNGO Software. Then, I followed the change in the bulk of duodenum and analyzed the changes in bulks of body on the same dose by transmitting the acquired KVCBCT into Pinnacle, a treatment plan system. The changes in the positioning shall be as set forth like this: 1.2 cm, 1.0 cm in Left-Right Direction, 0 cm, 0.8 cm in Craniocaudal Direction, 0.1 cm, and 1.0 cm in Anterior-Posterior Direction. Patient number one showed that his bulks in body had increased by maximum 460%, minimum 120%, the bulks in patient number two had increased by maximum 490%, minimum 160%, and the bulks of patient number three had increased by maximum 150%. But Minimum volume decreased 30%. Patient number one showed only a little bit of change at first when compared with the preliminary treatment plan. However, the dose increased the bulks in the patient's body: V{sub 10} 118%, V{sub 20} 117%, V{sub 30} 400%, and V{sub 40} 480% In treating patients with radiation therapy using 3D-CRT, the dose amount penetrated into duodenum needs to be minimized by planning appropriate treatment beforehand. In order to establish an appropriate treatment plan it is required to comprehend the changes at positioning of the duodenum by respiration and predict the changes in the bulks of duodenum by setting precise Planning Target Volume.

  8. Retinoic acid-activated Ndrg1a represses Wnt/β-catenin signaling to allow Xenopus pancreas, oesophagus, stomach, and duodenum specification.

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    Tiejun Zhang

    Full Text Available How cells integrate multiple patterning signals to achieve early endoderm regionalization remains largely unknown. Between gastrulation and neurulation, retinoic acid (RA signaling is required, while Wnt/β-catenin signaling has to be repressed for the specification of the pancreas, oesophagus, stomach, and duodenum primordia in Xenopus embryos. In attempt to screen for RA regulated genes in Xenopus endoderm, we identified a direct RA target gene, N-myc downstream regulated gene 1a (ndrg1a that showed expression early in the archenteron roof endoderm and late in the developing pancreas, oesophagus, stomach, and duodenum. Both antisense morpholino oligonucleotide mediated knockdown of ndrg1a in Xenopus laevis and the transcription activator-like effector nucleases (TALEN mediated disruption of ndrg1 in Xenopus tropicalis demonstrate that like RA signaling, Ndrg1a is specifically required for the specification of Xenopus pancreas, oesophagus, stomach, and duodenum primordia. Immunofluorescence data suggest that RA-activated Ndrg1a suppresses Wnt/β-catenin signaling in Xenopus archenteron roof endoderm cells. Blocking Wnt/β-catenin signaling rescued Ndrg1a knockdown phenotype. Furthermore, overexpression of the putative Wnt/β-catenin target gene Atf3 phenocopied knockdown of Ndrg1a or inhibition of RA signaling, while Atf3 knockdown can rescue Ndrg1a knockdown phenotype. Lastly, the pancreas/stomach/duodenum transcription factor Pdx1 was able to rescue Atf3 overexpression or Ndrg1a knockdown phenotype. Together, we conclude that RA activated Ndrg1a represses Wnt/β-catenin signaling to allow the specification of pancreas, oesophagus, stomach, and duodenum progenitor cells in Xenopus embryos.

  9. Central neuronal mechanisms of intestinal electrical stimulation: effects on duodenum distention-responsive (DD-R) neurons in the VMH of rats.

    Science.gov (United States)

    Zhang, Jing; Zhu, Hongbing; Chen, J D Z

    2009-06-19

    Intestinal electrical stimulation (IES) has been shown to produce inhibitory effects on gastric contractions, gastric emptying, food intake and body weight in rats and dogs, suggesting a therapeutic potential for obesity. The aims of this study were (1) to test the hypothesis that the neurons in the VMH are involved in the central mechanisms of IES treatment for obesity; (2) to compare the effects of IES at the duodenum and IES at the ileum on neuronal activities of the VMH; (3) to better understand if the neuronal activity modulated by IES was mediated via the vagal pathway. Extracellular potentials of neurons in the VMH were recorded in 18 anesthetized rats. IES at the duodenum or ileum was performed in duodenal-distention responsive (DD-R) neurons with 3 sets of parameters (IES-1 with trains of short-pulses: 4mA, 2s-on, 3s-off, 2ms, 20Hz; IES-2 with long-pulses: 6mA, 20cpm, 100ms; IES-3, same as IES-1 but 40Hz). IES-1 at the duodenum and the ileum activated 70.6% and 73.3% of the DD-R neurons, respectively. Similar percentages of the neurons were activated with IES-3 at the duodenum and the ileum (70.6% vs. 66.7%, P=0.91), respectively. IES-2 at these locations activated only 25% and 46.2% of the DD-R neurons, respectively (P>0.05). IES at the duodenum with parameter set, IES-1 or IES-3 was significantly more potent than the parameter set, IES-2 (neuronal activation: 70.6% vs. 25%, PDD, and IES with trains of short-pulses seems more effective than IES with long-pulses. The vagal pathway and extra-vagal pathways are involved in the modulatory effects of IES on the central neurons in the satiety center.

  10. Characterization of the myenteric neuronal population and subpopulation of the duodenum of adult wistar rat fed with hypoproteic chow

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    Débora M.G. Sant'Ana

    2012-09-01

    Full Text Available The effects of severe protein malnutrition (4% on myenteric neurons of Wistar rat duodenum, in relation to a standard 22%-protein diet for rodents, were assessed in this study. Segments of the duodenum from 10 rats from each nutritional group were submitted to the elaboration of whole mounts - 5 stained with Giemsa to determine the total population of myenteric neurons and the others stained by a histochemical method to detect nervous cells through the NADPH-diaphorase enzyme activity for studying the subpopulation of nitrergic neurons. The area of 100 neurons per animal, totalizing 2,000 neurons, were randomly measured by using the Image Pro-Plus®software. Malnourished rats presented 34.38% lower body weight and 10.60% duodenum length reduction when compared to the control group. Quantitative analysis demonstrated no significant differences between control and malnourished group by using Giemsa; however, as the organ reduction was not followed by an increase inversely proportional to the density of neurons, the condition imposed suggests the loss of neurons from the total population. Nevertheless, through NADPH-d histochemistry, there was a neuronal density increase for the malnourished group. There was no significant difference between the groups for both techniques with respect to the morphometric analysis of the body cell.Objetivou-se avaliar os efeitos da desnutrição protéica severa (4% sobre os neurônios mientéricos do duodeno de ratos Wistar, comparando-se a uma dieta padrão para roedores de 22% de proteína. Para este estudo, segmentos do duodeno de 10 ratos de cada grupo nutricional foram submetidos à elaboração de preparados de membrana, sendo cinco corados pelo método de Giemsa, para estimar a população mientérica total e os demais corados pelo método histoquímico de evidenciação de células nervosas, por meio da atividade da enzima NADPH-diaforase para estudo da subpopulação de neurônios nitr

  11. Immunohistochemical and ultrastructural characteristics of interstitial cells of Cajal in the rabbit duodenum. Presence of a single cilium.

    Science.gov (United States)

    Junquera, Concepción; Martínez-Ciriano, Carmen; Castiella, Tomás; Serrano, Pedro; Azanza, María Jesús; Junquera, Santiago Ramón y Cajal

    2007-01-01

    Santiago Ramón y Cajal discovered a new type of cell related to the myenteric plexus and also to the smooth muscle cells of the circular muscle layer of the intestine. Based on their morphology, relationships and staining characteristics, he considered these cells as primitive neurons. One century later, despite major improvements in cell biology, the interstitial cells of Cajal (ICCs) are still controversial for many researchers. The aim of study was to perform an immunohistochemical and ultrastructural characterization of the ICCs in the rabbit duodenum. We have found interstitial cells that are positive for c-Kit, CD34 and nestin and are also positive for Ki67 protein, tightly associated with somatic cell proliferation. By means of electron microscopy, we describe ICCs around enteric ganglia. They present triangular or spindle forms and a very voluminous nucleus with scarce perinuclear chromatin surrounded by a thin perinuclear cytoplasm that expands with long cytoplasmic processes. ICC processes penetrate among the smooth muscle cells and couple with the processes of other ICCs located in the connective tissue of the circular muscle layer and establish a three-dimensional network. Intercellular contacts by means of gap-like junctions are frequent. ICCs also establish gap-like junctions with smooth muscle cells. We also observe a population of interstitial cells of stellate morphology in the connective tissue that sur-rounds the muscle bundles in the circular muscle layer, usually close to nervous trunks. These cells establish different types of contacts with the muscle cells around them. In addition, the presence of a single cilium showing a structure 9 + 0 in an ICC is demonstrated for the first time. In conclusion, we report positive staining c-Kit, CD34, nestin and Ki 67. ICCs fulfilled the usual transmission electron microscopy (TEM) criteria. A new ultrastructural characteristic of at least some ICCs is demonstrated: the presence of a single cilium. Some

  12. Interleukin-1 modulatory effect on the action of chemotherapeutic drugs and localized irradiation of the lip, duodenum, and tumor

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    Zaghloul, M.S.; Dorie, M.J.; Kallman, R.F. (Stanford Univ. School of Medicine, Stanford, CA (United States))

    1993-06-15

    This study was conducted to examine the radioprotective and radiochemoprotective capabilities of interleukin 1[beta] (IL-1) on two acute-reacting normal tissues of the C3H mouse, the mucosa of the lip and the duodenum. Also assessed was the modulating effect of IL-1 on tumor growth in the same strain of mice. IL-1 was administered to C3H-Km mice in combination with fractionated irradiation, or with cyclophosphamide, cisplatin, or 5-fluorouracil (5FU) followed by irradiation. Normal tissue damage was evaluated in the mouse lip, using a subjective scoring system for tissue reaction, and in the duodenum, using the crypt cell survival assay. RIF-1 fibrosarcoma tumor response was assayed with the regrowth delay method. IL-1 protected against the acute reaction produced by fractionated irradiation in the lip mucosa, shifting the dose-response curve by 3.8 Gy. IL-1 was protective when injected intraperitoneally 24 hr before CY or c-DDP, which were given immediately before the first of five daily radiation dose fractions. The dose-response curves for cyclophosphamide and cisplatin were shifted 4.0 Gy and 1.6 Gy, respectively. IL-1 did not protect against 5FU toxicity when treatments were administered in that same sequence; however, when 5FU was given 4 or 8 hr before IL-1 and the first radiation dose fraction followed 20 or 16 hr later, there was significant protection and the curves were separated by 1.5 Gy or 3.5 Gy. IL-1 also protected duodenal crypt cells against the cytocidal effect of fractionated irradiation, with a dose difference of 1.5 Gy and an improvement of crypt survival of 11.7%. It was even more immediately before the first of five daily radiation doses, with the dose differences of 4.4 and 5.3 Gy, respectively, and improvements of crypt survival of 33.8 and 29.9%, respectively. There was no modification by IL-1 of the effect of irradiation alone on the RIF-1 tumor. 45 refs., 8 figs., 1 tab.

  13. Amino acid limitation and flow to the duodenum at four stages of lactation. 2. Extent of lysine limitation.

    Science.gov (United States)

    Schwab, C G; Bozak, C K; Whitehouse, N L; Olson, V M

    1992-12-01

    Four multiparous Holstein cows with ruminal and duodenal cannulas were assigned to 4 x 4 Latin squares at peak (wk 4), early (wk 14 to 16), mid (wk 21 to 23), and late (wk 29 to 31) lactation to determine, in the presence of supplemental Met, the extent of Lys limitation and its required contribution to total essential AA in duodenal digesta. Treatments were duodenal infusions of 1) water alone or water with 2) 10 g/d of DL-Met plus 10 g/d of L-Lys, 3) 10 g/d of Met plus 20 g/d of Lys, and 4) 10 g/d of Met plus 30 g/d of Lys; quantities were reduced by 20% in late lactation. Rations were corn based (corn and grass-legume silages, corn meal, wheat middlings, soybean meal, and distillers dried grains with solubles) and most limiting in Lys and Met. Intakes of ruminally degraded and undegraded intake protein (percentage of NRC requirements) were (peak) 115, 97; (early) 112, 83; (mid) 113, 87; and (late) 127, 96. Contribution of Lys to passage of total essential AA to the duodenum without infusions were 13.2, 12.4, 13.8, and 14.8% at the four respective stages of lactation. Extent of Lys limitation determined from responses in content and yield of milk protein approximated 25, 20, and 10 g/d during peak, early, and midlactation.(ABSTRACT TRUNCATED AT 250 WORDS)

  14. Duodenum inclusion in alimentary transit for preventing or correcting nutritional deficiencies resulting from Roux-en-y gastric bypass in obesity treatment

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    REGINALDO CENEVIVA

    2016-04-01

    Full Text Available ABSTRACT Nutritional and metabolic complications can develop after Roux-en-Y gastric bypass (RYGB when there is an exaggerated response to the anatomical and functional changes or when there is inadequate nutritional supplementation. Severe malnutrition is rare, but deficiencies of vitamin B12, iron, calcium and thiamin, metabolic bone disease and gallstones are common after RYGB. Shortage of vitamin B12, iron, calcium and also cholelithiasis are caused at least partially by excluding the duodenum and proximal jejunum from food transit. We designed a new procedure, with the maintenance of the duodenum and proximal jejunum in the gastrointestinal transit through interposition of jejunal loop, as a primary operation to prevent such deficiencies or as corrective surgery for severe malnutrition after RYGB with failure in responding to conservative treatment.

  15. Synchronous Occurrence of Diffuse Large B-cell Lymphoma of the Duodenum and Gastrointestinal Stromal Tumor of the Ileum in a Patient with Immune Thrombocytopenic Purpura

    Science.gov (United States)

    Takahashi, Tohru; Maruyama, Yumiko; Saitoh, Mayuko; Itoh, Hideto; Yoshimoto, Mitsuru; Tsujisaki, Masayuki; Nakayama, Masato

    2016-01-01

    A 64 year-old woman with steroid-dependent immune thrombocytopenia developed anemia. Esophagogastroduodenoscopy revealed the presence of a tumor, which was diagnosed to be diffuse large B-cell lymphoma, in the second portion of the duodenum. 18F-fluorodeoxy glucose positron emission tomography showed an increased uptake mass in the pelvic cavity as well as in the duodenum. Though the duodenal tumor disappeared after 4 cycles of chemotherapy, the pelvic mass did not shrink in size. As a result, laparoscopic resection of the pelvic tumor was performed and the tumor was histologically diagnosed to be a gastrointestinal stromal tumor. Subsequently, the patient was treated with 2 more cycles of the chemotherapy. Eventually, thrombocytopenia completely resolved. PMID:27746431

  16. CARCINOID TUMOR OF THE DUODENUM: a rare tumor at an unusual site. Case series from a single institution

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    Jaques WAISBERG

    2013-03-01

    Full Text Available Context Duodenal carcinoids are extremely rare, and their characteristics and biological behavior have not been fully elucidated. Objective To analyze the clinicopathological characteristics of patients with resected duodenal carcinoids. Methods Twenty patients (12 females and 8 males were investigated. Their average age was 66.4 ± 5.8 years old (43 to 88 years old. The data corresponding to the clinical picture, diagnosis, treatment, and prognosis of patients with duodenal carcinoid tumors subjected to resection over a period of 18 years (1993-2011 were analyzed. Results The most common symptoms were dyspepsia (50% and epigastric pain (45% followed by weight loss (10% and vomiting (5%. Carcinoid syndrome was not observed in any patient. The lesion was located on the first part of the duodenum in 15 (75% patients, the second part in 4 (20% patients, and the third part in 1 (5% patient. The diagnosis of a carcinoid tumor was established through an endoscopic excision biopsy in 19 (95% patients and an histopathological examination of the surgical specimen in 1 (5% patient. The average tumor size was 1.1 cm ± 0.4 cm (0.3 cm to 6.0 cm. Nineteen (95% patients were initially treated by endoscopic resection of the duodenal lesion. One patient (5%, whose tumor was on the third part of the duodenum underwent a duodenectomy of the third and fourth duodenal parts and duodenojejunal anastomosis. The duodenal carcinoid resection margin was involved in four (20% patients. Four (20% patients were subjected to a partial gastrectomy to fully remove the lesion. The tumor was restricted to the submucosal layer in 16 (80% cases, and it penetrated into the muscular layer in 4 (20% cases. All patients exhibited positive chromogranin A, neuron-specific enolase, and/or synaptophysin immunostaining. The average duration of the follow-up period was 39.6 months (3 to 96 months. Twelve (60% of the 20 cases in this series are alive without any evidence of active

  17. Lipidomic and spatio-temporal imaging of fat by mass spectrometry in mice duodenum during lipid digestion.

    Science.gov (United States)

    Seyer, Alexandre; Cantiello, Michela; Bertrand-Michel, Justine; Roques, Véronique; Nauze, Michel; Bézirard, Valérie; Collet, Xavier; Touboul, David; Brunelle, Alain; Coméra, Christine

    2013-01-01

    Intestinal absorption of dietary fat is a complex process mediated by enterocytes leading to lipid assembly and secretion of circulating lipoproteins as chylomicrons, vLDL and intestinal HDL (iHDL). Understanding lipid digestion is of importance knowing the correlation between excessive fat absorption and atherosclerosis. By using time-of-flight secondary ion mass spectrometry (TOF-SIMS), we illustrated a spatio-temporal localization of fat in mice duodenum, at different times of digestion after a lipid gavage, for the first time. Fatty acids progressively increased in enterocytes as well as taurocholic acid, secreted by bile and engaged in the entero-hepatic re-absorption cycle. Cytosolic lipid droplets (CLD) from enterocytes were originally purified separating chylomicron-like, intermediate droplets and smaller HDL-like. A lipidomic quantification revealed their contents in triglycerides, free and esterified cholesterol, phosphatidylcholine, sphingomyelin and ceramides but also in free fatty acids, mono- and di-acylglycerols. An acyl-transferase activity was identified and the enzyme monoacylglycerol acyl transferase 2 (MGAT2) was immunodetected in all CLD. The largest droplets was also shown to contain the microsomal triglyceride transfer protein (MTTP), the acyl-coenzyme A-cholesterol acyltransferases (ACAT) 1 and 2, hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL). This highlights the fact that during the digestion of fats, enterocyte CLD contain some enzymes involved in the different stages of the metabolism of diet fatty acids and cholesterol, in anticipation of the crucial work of endoplasmic reticulum in the process. The data further underlines the dual role of chylomicrons and iHDL in fat digestion which should help to efficiently complement lipid-lowering therapy.

  18. 16S rRNA gene pyrosequencing reveals bacterial dysbiosis in the duodenum of dogs with idiopathic inflammatory bowel disease.

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    Jan S Suchodolski

    Full Text Available BACKGROUND: Canine idiopathic inflammatory bowel disease (IBD is believed to be caused by a complex interaction of genetic, immunologic, and microbial factors. While mucosa-associated bacteria have been implicated in the pathogenesis of canine IBD, detailed studies investigating the enteric microbiota using deep sequencing techniques are lacking. The objective of this study was to evaluate mucosa-adherent microbiota in the duodenum of dogs with spontaneous idiopathic IBD using 16 S rRNA gene pyrosequencing. METHODOLOGY/PRINCIPAL FINDINGS: Biopsy samples of small intestinal mucosa were collected endoscopically from healthy dogs (n = 6 and dogs with moderate IBD (n = 7 or severe IBD (n = 7 as assessed by a clinical disease activity index. Total RNA was extracted from biopsy specimens and 454-pyrosequencing of the 16 S rRNA gene was performed on aliquots of cDNA from each dog. Intestinal inflammation was associated with significant differences in the composition of the intestinal microbiota when compared to healthy dogs. PCoA plots based on the unweighted UniFrac distance metric indicated clustering of samples between healthy dogs and dogs with IBD (ANOSIM, p<0.001. Proportions of Fusobacteria (p = 0.010, Bacteroidaceae (p = 0.015, Prevotellaceae (p = 0.022, and Clostridiales (p = 0.019 were significantly more abundant in healthy dogs. In contrast, specific bacterial genera within Proteobacteria, including Diaphorobacter (p = 0.044 and Acinetobacter (p = 0.040, were either more abundant or more frequently identified in IBD dogs. CONCLUSIONS/SIGNIFICANCE: In conclusion, dogs with spontaneous IBD exhibit alterations in microbial groups, which bear resemblance to dysbiosis reported in humans with chronic intestinal inflammation. These bacterial groups may serve as useful targets for monitoring intestinal inflammation.

  19. A case report of duodenum lymphangioma and literature review%十二指肠淋巴管瘤1例并文献复习

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    李素娟; 苏秉忠; 杜华

    2015-01-01

    Objective: To investigate the clinicopathologic characteristics, diagnosis, treatment and prognosis of lymphangioma of duodenum.Methods: One case of lymphangioma at descending duodenum in adult was analyzed by means of gastroscopy and immunohistochemical examination with review of the 1iterature. Results:A 68-year-old female, presenting with abdominal discomfort, was diagnosed as duodenum lymphangioma. hTere was an irregular milky granular polypoid lesions in descending duodenum by gastroscopy. The pathological diagnosis was irregularly expanded lymphatics within intestinal muscular layer, of which the lumens contained lymph. Immunohistochemically, vascular epithelia were positive for D2-40, negative for CD34. Regular follow-up of patient, and endoscopic morphology did not change significantly after one year. Conclusion: Lymphangioma in descending duodenum is rare in adults. For patients with atypical symptoms of the upper abdomen, duodenal descending lesions should be considered the possibility of lymphangioma, and should be given individual treatment according to different clinical manifestations.%目的:探讨十二指肠淋巴管瘤的临床病理特点、诊断、治疗及预后。方法:1例上腹不适的患者除一般的血清学、尿液、腹部CT等检查外,接受胃镜检查并取粘膜行病理活检。结果:胃镜示十二指肠降部见不规则乳白色颗粒样隆起,病理示粘膜固有层见大小不一扩张的淋巴管,其腔内见淋巴液。免疫组化CD34阴性,D2-40阳性。患者定期随访,一年后内镜下形态未见明显改变。结论:十二指肠淋巴管瘤在成人中可能并不多见。对于上腹部不典型症状,十二指肠降部见隆起型病变的患者应考虑到淋巴管瘤的可能性。并根据不同临床表现个体化治疗。

  20. Effect of fluoxetine on depression-induced changes in the expression of vasoactive intestinal polypeptide and corticotrophin releasing factor in rat duodenum

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    AIM: To investigate changes in vasoactive intestinal polypeptide (VIP) and corticotrophinreleasing factor (CRF) in the plasma and duodenum of chronic stressinduced depressed rats and the effects of fluoxetine hydrochloride (fluoxetine) treatment on depressioninduced changes in VIP and CRF.METHODS: A Sprague-Dawley rat model of chronic stress-induced depression was produced. Thirty experimental rats were randomly divided into the following groups: control group, saline-treated depressed group, and fluoxetine-treated depressed group. Openfield testing was performed to assess the rats' behavior.VIP and CRF levels in plasma were measured by ELISA.Immunofluorescence techniques combined with laser scanning confocal microscopy (LSCM) were used to investigate VIP and CRF expression in the duodenum.RESULTS: The open-field behavior, both crossing and rearing, of depression model rats, decreased significantly compared with those of normal control rats over 5 min.Defecation times increased significantly. Compared to the control group, FITC fluorescence of duodenal CRF expression and plasma CRF levels in the depressed rats increased significantly (fluorescence intensity of duodenal CRF: 11.82 ± 2.54 vs 25.17 ± 4.63; plasma CRF: 11.82 ± 2.54 ng/L vs 25.17 ± 4.63 ng/L, P<0.01),whereas duodenal VIP expression and plasma VIP levels decreased significantly (fluorescence intensity of duodenal VIP: 67.37 ± 18.90 vs 44.51 ± 16.37; plasma VIP: 67.37 ± 18.90 ng/L vs 44.51±16.37 ng/L, P < 0.01).Fluoxetine improved depressed behavior, increased VIP expression and decreased CRF expression in plasma and the duodenal tissue of depressed rats.CONCLUSION: Chronic stress can induce injury to the duodenum, accompanied by increasing CRF and decreasing VIP in the plasma and duodenum. Treatment with fluoxetine can ameliorate pathological changes in the duodenum of depressed rats, which suggests that antidepressants are an effective therapeutic agent for some duodenal diseases caused by

  1. Stomach and duodenum

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    2008439 The effects of H.pylori and its crude extracted proteins on isolated rabbit parietal cells acid secretion.GUO Tao(郭涛), et al. Dept Gastroenterol, PUMC & CAMS, Beijing 100730. Chin J Intern Med 2008;47(7):566-569. Objective To explore the effects of H. pylori and crude extracted proteins secreted byH. pylori (broth culture filtrate protein, BCF-P) on acid secretion from isolated rabbit parietal cells.

  2. Stomach and duodenum

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    2009195 The expression of heat shock protein 70/90 in patients with atrophic gastritis or gastric cancer and its significance.SUN Leimin(孙蕾民),et al.Dept Gastroenterol,Sir Run Run Shaw Hosp,Zhejiang Univ,Hangzhou 310016.Chin J Dig,2009;29(3);164-167.

  3. Stomach and duodenum

    Institute of Scientific and Technical Information of China (English)

    1992-01-01

    920683 The relationship between the fast-ing gastric bile acids and non-ulcer dyspep-sia. FENG Weihua(冯伟华), et al. Dept Bio-chemist, Changhai Hosp, 2nd Milit Med Coll.Shanghai 200433 Chin. J Digest 1992; 12(2):77-79. In the present study, the concentrations ofthe fasting gastric bile acid from 49 patients with

  4. Stomach and duodenum

    Institute of Scientific and Technical Information of China (English)

    1995-01-01

    950320 Light and electron microscopic histochemicalstudies on alkaline phosphatase (ALP) isoenzymes ingastric cancer.SU Yinghao(苏英豪),et al.DeptPathol,Anhui Med Univ,Hefei,230032.Chin J Pathol1994;23(6):338-340.By using light and electron microscopic histochemi-cal techniques,the activities and distributions of ALPisoenzymes in gastric cancers and benign gastric dis-eases were examined.The results showed:Nagao,Re-gan and Kasahara isoenzymes,which were not ex-pressed in normal gastric mucosa and non-malignant

  5. Recurrent Renal Cell Carcinoma with Synchronous Tumor Growth in Azygoesophageal Recess and Duodenum: A Rare Cause of Anemia and Upper Gastrointestinal Bleeding

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    Vamshidhar R. Vootla

    2015-01-01

    Full Text Available Renal cell carcinoma (RCC has potential to present with distant metastasis several years after complete resection. The common sites of metastases include the lungs, bones, liver, renal fossa, and brain. RCCs metastasize rarely to the duodenum, and duodenal metastasis presenting with acute gastrointestinal bleed is infrequently reported in literature. We present a case of synchronous presentation of duodenal and azygoesophageal metastasis manifesting as acute upper gastrointestinal bleeding, four years after undergoing nephrectomy for RCC. The patient underwent further workup and was treated with radiation. The synchronous presentation is rare and stresses the importance of searching for recurrence of RCC in patients presenting with acute gastrointestinal bleeding.

  6. CARCINOID TUMOR OF THE DUODENUM: a rare tumor at an unusual site. Case series from a single institution

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    Jaques WAISBERG

    2013-03-01

    Full Text Available Context Duodenal carcinoids are extremely rare, and their characteristics and biological behavior have not been fully elucidated. Objective To analyze the clinicopathological characteristics of patients with resected duodenal carcinoids. Methods Twenty patients (12 females and 8 males were investigated. Their average age was 66.4 ± 5.8 years old (43 to 88 years old. The data corresponding to the clinical picture, diagnosis, treatment, and prognosis of patients with duodenal carcinoid tumors subjected to resection over a period of 18 years (1993-2011 were analyzed. Results The most common symptoms were dyspepsia (50% and epigastric pain (45% followed by weight loss (10% and vomiting (5%. Carcinoid syndrome was not observed in any patient. The lesion was located on the first part of the duodenum in 15 (75% patients, the second part in 4 (20% patients, and the third part in 1 (5% patient. The diagnosis of a carcinoid tumor was established through an endoscopic excision biopsy in 19 (95% patients and an histopathological examination of the surgical specimen in 1 (5% patient. The average tumor size was 1.1 cm ± 0.4 cm (0.3 cm to 6.0 cm. Nineteen (95% patients were initially treated by endoscopic resection of the duodenal lesion. One patient (5%, whose tumor was on the third part of the duodenum underwent a duodenectomy of the third and fourth duodenal parts and duodenojejunal anastomosis. The duodenal carcinoid resection margin was involved in four (20% patients. Four (20% patients were subjected to a partial gastrectomy to fully remove the lesion. The tumor was restricted to the submucosal layer in 16 (80% cases, and it penetrated into the muscular layer in 4 (20% cases. All patients exhibited positive chromogranin A, neuron-specific enolase, and/or synaptophysin immunostaining. The average duration of the follow-up period was 39.6 months (3 to 96 months. Twelve (60% of the 20 cases in this series are alive without any evidence of active

  7. Influence of substitution at C/sub 24/ on the calcium-binding protein-stimulating activity of vitamin D metabolites in chick embryonic duodenum

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    Parkes, C.O. (Univ. of British Columbia, Vancouver); Deluca, H.F.

    1979-01-01

    The production of calcium-binding protein, in vitro, by embryonic chick duodenum has been used to assess the potency of vitamin D compounds. The introduction of an hydroxyl on 1-, 25-, or 24R-position enhanced biological activity while the introduction of both 1..cap alpha..- and 25-hydroxyls produced maximal activity. However, 24R-hydroxylation of 1,25-dihydroxyvitamin D/sub 3/ diminished activity. The vitamin D/sub 2/ side chain on 25-hydroxy-vitamin D or 1,25-dihydroxyvitamin D did not greatly diminish activity in contrast to the fact that the vitamin D/sub 2/ compounds are 10% as active as the vitamin D/sub 3/ compounds in vivo in the chick. These results support the idea that the target organs of the chick do not discriminate against the vitamin D/sub 2/ side chain and that the discrimination in this species is at the level of metabolism.

  8. Operation approaches for colon cancer invading duodenum%结肠癌侵犯十二指肠的手术方式探讨

    Institute of Scientific and Technical Information of China (English)

    王坤; 刘伟昌; 施海

    2016-01-01

    Objective To investigate the operation approaches of colon cancer invading duodenum and to evaluate the clinical effect. Methods A retrospective analysis of twenty six cases of colorectal cancer patients admitted to Xijing Hospital from January 2013 to December 2014 were analyzed according to different clinical features, surgical methods and the survival of the patients. Results Radical right hemicolectomy was carried out in 8 cases, the radical right hemicolectomy resection combined with duodenal wall defect discontinuous full-thickness suture in 10 cases, and 4 cases underwent right hemicolectomy, duodenum jejunum end to side anastomosis + jejunum Roux-en-Y anastomosis, jejunum nutrition tube fistulation. 1 patient underwent right hemicolectomy, pancreas duodenum to intestinal resection, radical resection, resection of the gastric antrum and duodenal bulb, and 1 case of gastrojejunal anastomosis of the colon. The other 2 cases were given transverse colon and ileum side to side anastomosis. 1 patient had postoperative colon ileum anastomotic fistula. 12-month survival rate was 84.6%, and 30-month survival rate was 76.9%. Conclusion Reasonable imaging evaluation, aggressive surgical treatment combined with the right surgical methods can improve the prognosis of patients with colon cancer invading duodenum.%目的:探讨结肠癌侵犯十二指肠的手术方式,评估临床疗效。方法回顾性分析第四军医大学附属西京医院2013年1月至2014年12月收治的结肠癌侵犯十二指肠患者共26例,结合患者不同的临床特点及手术方式进行生存分析。结果根治性右半结肠切除术8例,根治性右半结肠切除术+十二指肠壁缺损间断全层缝合术10例,右半结肠切除、空肠十二指肠端侧吻合+空肠Roux-en-Y吻合、空肠营养管造瘘术4例,右半结肠切除、胰十二指肠切除术1例,根治性右半结肠切除、胃窦及球部切除、胃空肠吻合术1例,

  9. Effect of niacin supplementation on rumen fermentation characteristics and nutrient flow at the duodenum in lactating dairy cows fed a diet with a negative rumen nitrogen balance.

    Science.gov (United States)

    Aschemann, Martina; Lebzien, Peter; Hüther, Liane; Südekum, Karl-Heinz; Dänicke, Sven

    2012-08-01

    The aim of the present experiment was to ascertain if a daily niacin supplementation of 6 g/cow to lactating dairy cow diets can compensate for the decrease in rumen microbial fermentation due to a negative rumen nitrogen balance (RNB). A total of nine ruminally and duodenally fistulated lactating multiparous German Holstein cows was used. The diets consisted of 10 kg dry matter (DM) maize silage and 7 kg DM concentrate and differed as follows: (i) Diet RNB- (n = 6) with energy and utilisable crude protein (CP) at the duodenum (uCP) according to the average requirement of the animals, but with a negative RNB (-0.41 g N/MJ metabolisable energy [ME]); (ii) Diet RNB0 (n = 7) with energy, uCP, and RNB (0.08 g N/MJ ME) according to the average requirement of the animals; and (iii) Diet NA (nicotinic acid; n = 5), which was the same diet as RNB-, but supplemented with 6 g niacin/d. The negative RNB affected the rumen fermentation pattern and reduced ammonia content in rumen fluid and the daily duodenal flows of microbial CP (MP) and uCP. Niacin supplementation increased the apparent ruminal digestibility of neutral detergent fibre. The efficiency of microbial protein synthesis per unit of rumen degradable CP was higher, whereby the amount of MP reaching the duodenum was unaffected by niacin supplementation. The number of protozoa in rumen fluid was higher in NA treatment. The results indicated a more efficient use of rumen degradable N due to changes in the microbial population in the rumen when niacin was supplemented to diets deficient in RNB for lactating dairy cows.

  10. The Clinical Application of the Duodenum Closed Exclusion%十二指肠缝闭式旷置术临床应用研究

    Institute of Scientific and Technical Information of China (English)

    张钦增; 侯化丽; 张利标; 金鑫; 孙宗林

    2013-01-01

      目的:比较幽门旷置十二指肠憩室化各种术式,探讨十二指肠缝闭式旷置术的临床应用可能性及方法。方法:通过近12年32例十二指肠缝闭式旷置术患者的临床资料分析,对比其他手术方法,观察手术并发症、手术时间及术后恢复情况。结果:32例患者中2例出现胃瘫,经禁食、胃肠减压、肠内外营养等,分别与本次手术后第15天、21天恢复;1例切口感染,经换药痊愈;1例胃癌晚期患者出院后半年因恶性消耗衰竭而亡,其余患者恢复顺利,无近期与手术有关死亡病例。结论:十二指肠缝闭式旷置术术式简便、缝闭准确、并发症少、手术时间短、创伤小、更符合损伤控制原则,尤其适用于损伤严重、年老体弱及术中生命体征不平稳者。%Objective:To compare various duodenal diverticulization operation with pylorus exclusion,investigate the possibility of clinical application and method of the duodenum closed exclusion. Method:According to the clinical data of 32 cases with the duodenum closed exclusion during the past 4 years,to compare with others technique,observe the complication,operative time and postoperative recovery. Result:In the 32 cases,2 cases recovered in the postoperative fifteenth day and twenty-first day which occured gastroplegia. 1 case occurred postoperative infection and recovered after change dressings,1 case with advanced stage of gastric carcinoma died after half a year,because of cachexy. Others successfully recovered,and there was no dead case who was relevant to operation in the near future. Conclusion:The duodenum closed exclusion is convenient and accurate,the trauma is little and complication is few,the operative time is short,it is more coincident with the principle of damage control,especially applies to these cases who are aged and physically weak,injure severely and don’t have stable vital sign during operation.

  11. Duodenum inclusion in alimentary transit for preventing or correcting nutritional deficiencies resulting from Roux-en-y gastric bypass in obesity treatment.

    Science.gov (United States)

    Ceneviva, Reginaldo

    2016-01-01

    Nutritional and metabolic complications can develop after Roux-en-Y gastric bypass (RYGB) when there is an exaggerated response to the anatomical and functional changes or when there is inadequate nutritional supplementation. Severe malnutrition is rare, but deficiencies of vitamin B12, iron, calcium and thiamin, metabolic bone disease and gallstones are common after RYGB. Shortage of vitamin B12, iron, calcium and also cholelithiasis are caused at least partially by excluding the duodenum and proximal jejunum from food transit. We designed a new procedure, with the maintenance of the duodenum and proximal jejunum in the gastrointestinal transit through interposition of jejunal loop, as a primary operation to prevent such deficiencies or as corrective surgery for severe malnutrition after RYGB with failure in responding to conservative treatment. Complicações nutricionais e metabólicas podem se desenvolver após a derivação gástrica em Y de Roux (DGYR) quando há uma resposta exagerada às mudanças anatômicas e funcionais ou quando há suplementação nutricional inadequada. A desnutrição grave é rara, mas deficiências de vitamina B12, ferro, cálcio e tiamina, doença óssea metabólica e cálculos biliares são comuns após a DGYR. Dessas deficiências mencionadas, a de vitamina B12, de ferro, de cálcio e também a colelitíase, são causadas, ao menos parcialmente, pela exclusão do duodeno e jejuno proximal. Um novo procedimento com a manutenção do duodeno e do jejuno proximal no trânsito gastrointestinal, mediante interposição de alça jejunal, foi idealizado como operação primária para prevenir essas deficiências ou como cirurgia corretiva de desnutrição grave após DGYR com falha na resposta a exaustivas tentativas de tratamento conservador.

  12. Pancreatite aguda devida a hematoma intramural do duodeno por uso de anticoagulante Acute pancreatitis due to intramural hematoma of the duodenum by use of anticoagulant therapy

    Directory of Open Access Journals (Sweden)

    Samer FARHOUD

    2001-01-01

    Full Text Available Racional - A hemorragia intramural espontânea do duodeno causada por complicações da terapêutica anticoagulante é rara e seu tratamento controverso. Objetivo -- Apresentar a experiência advinda do tratamento de doente com essa condição clínica. Relato do caso - Expõe-se o caso de uma mulher de 71 anos de idade, que há 3 meses fazia uso de anticoagulante oral para tratamento de trombose venosa profunda dos membros inferiores. Apresentou-se com cefaléia e dores abdominais intensas no andar superior do abdome, associadas a náuseas e vômitos. Os exames laboratoriais e de imagem comprovaram o diagnóstico de surto agudo de pancreatite, decorrente de hematoma intramural de duodeno. Os valores de protrombina (49,7 s e o sangramento de tecidos moles cervicais e urinário, sugeriam complicação da terapêutica anticoagulante. Resultados - A terapêutica conservadora foi efetiva, tendo a doente recebido alta, assintomática, no 10º dia de internação. Conclusão - É recomendado o emprego do anticoagulante em doses menores nos doentes de risco e adequado controle dos parâmetros da coagulação. Acredita-se ser ideal a conduta conservadora e recomenda-se a cirurgia somente nos casos que evoluem com complicações.Background - Spontaneous intramural hemorrhage of the duodenum due to anticoagulant therapy is rare and the treatment is controversial. Objective - To present the acquired knowledge with the treatment of these disease. Case report - A 71-year-old women receiving for a 3 month period an anticoagulant therapy presented cervical bleeding of soft tissues and symptoms of acute pancreatitis and high small bowel obstruction. Early noninvasive diagnosis by computed tomographic scan was possible and conservative therapy proved successful in complete resolution of the pancreatitis and obstructive symptoms, with resumption of oral intake in the fourth day of treatment. The frequency of bleeding in high risk patients during warfarin therapy

  13. The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums.

    Science.gov (United States)

    Ragone, María Inés; Sella, Mariana; Conforti, Paula; Volonté, María G; Consolini, Alicia E

    2007-09-05

    The spasmolytic effects of an acqueous extract of cedrón (AEC) were studied on rat isolated duodenums. This plant (Aloysia citriodora Palau, Verbenaceae) is widely used for gastrointestinal disorders and as eupeptic in South America. AEC non-competitively inhibited the dose-response curve (DRC) of Ach (IC50 of 1.34 +/- 0.49 mg lyophilized/mL) and the DRC of Ca(2+) in high-[K(2-)](o) (IC50 of 2.64 +/- 0.23 mg/mL). AEC potentiated the non-competitive inhibition of either 30 micromol/L W-7 (a calmodulin blocker) and 5-15 micromol/L papaverine on the Ca(2+)-DRC. Also, AEC relaxed the contracture produced by high-[K(+)](o) (IC50 of 2.6 +/- 0.2 mg/mL) until 81.0 +/- 3.2% of the maximal effect of papaverine and 78.1+/- 5.0% of the quercetin, the most selective inhibitor of PDE. The AEC relaxation was non-competitively inhibited by 10-30 micromol/L methylene blue and competitively antagonized by 40 mmol/L TEA. The relaxation of 1mg/mL AEC was inhibited by hypoxia, but not that of 2mg/mL. Two flavonoids were identified by HPLC in the AEC: vitexin and isovitexin. Vitexin non-competitively inhibited the Ach-DRC (pD(2') of 5.7 +/- 0.4) but significantly run leftward the DRC of Ca(2+). Isovitexin did not significantly inhibit the DRC of Ach nor Ca(2+). The results suggest that the spasmolytic effect of AEC could be mostly associated to the increase in cGMP (target shared with the PDE inhibitors) and the activation of K(+)-channels. At low concentrations, AEC also inhibits the aerobic metabolism. The flavonoid vitexin is partially responsible for the effect, since it non-competitively inhibits Ach but not the Ca(2+) influx. Isovitexin was devoid of activity on duodenums.

  14. Carcinoma Arising from Brunner’s Gland in the Duodenum after 17 Years of Observation – A Case Report and Literature Review

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    Masaru Koizumi

    2007-10-01

    Full Text Available A 60-year-old man presented with melena and hematemesis in 1984. Esophagogastroduodenoscopy (EGD detected a small protruding lesion in the duodenal bulb, which was diagnosed as Brunner’s adenoma. No significant change was detected in subsequent annual EGD and biopsies for 10 years, after which the patient was not observed for 7 years. The patient presented with melena again in 2001. The lesion had changed shape to become a 10 mm sessile tumor with a central depression, and following a biopsy was diagnosed as an adenocarcinoma. The patient underwent partial resection of the duodenum. Histopathological assessment showed acidophilic cells with swollen nuclei, and clear cells forming a tubular or papillary tubule in the mucosal lamina propria and submucosal layer. The tumor cells stained positive for lysozyme, indicating that they arose from Brunner’s gland. The patient showed no sign of recurrence and was disease-free for more than 34 months after surgery. The patient died of pneumonia. This is an extremely rare case of primary duodenal carcinoma arising from Brunner’s gland in a patient observed for 17 years.

  15. Primary Pure Squamous Cell Carcinoma of the Gallbladder Locally Invading the Liver, Duodenum, and Stomach: A Case Report and Literature Review

    Science.gov (United States)

    Alpuerto, Aldrin C.; Mora, Maximo E.

    2017-01-01

    Primary pure squamous cell carcinoma (SCC) of the gallbladder is an exceptionally rare type of tumor that comprises only 1% of all gallbladder cancer. SCC of the gallbladder portends a worse prognosis than the more common adenocarcinoma variant because of its aggressive invasion to local structures and because it is often diagnosed at an advanced stage. Owing to its rarity, diagnosis and management can be challenging. Herein, we present the case of a 75-year-old female complaining of abdominal pain, nausea, and vomiting. Computed tomography and ultrasonography results of the abdomen were consistent with acute cholecystitis and cholelithiasis. Histologic evaluation of the resected mass revealed a malignant tumor with prominent keratinization, confirming the diagnosis of an invasive primary pure SCC of the gallbladder. Microscopic examination showed direct infiltration to the liver, duodenum, and stomach. This case report describes the hospital course of a patient with SCC of the gallbladder and suggests that gallbladder cancer should be considered as part of the differential diagnosis in elderly patients presenting with acute cholecystitis. In addition, this article will review existing literature to examine the utility of different diagnostic techniques and treatment modalities available in the management of gallbladder cancer. PMID:28251014

  16. Primary Pure Squamous Cell Carcinoma of the Gallbladder Locally Invading the Liver, Duodenum, and Stomach: A Case Report and Literature Review

    Directory of Open Access Journals (Sweden)

    Aldrin C. Alpuerto

    2017-01-01

    Full Text Available Primary pure squamous cell carcinoma (SCC of the gallbladder is an exceptionally rare type of tumor that comprises only 1% of all gallbladder cancer. SCC of the gallbladder portends a worse prognosis than the more common adenocarcinoma variant because of its aggressive invasion to local structures and because it is often diagnosed at an advanced stage. Owing to its rarity, diagnosis and management can be challenging. Herein, we present the case of a 75-year-old female complaining of abdominal pain, nausea, and vomiting. Computed tomography and ultrasonography results of the abdomen were consistent with acute cholecystitis and cholelithiasis. Histologic evaluation of the resected mass revealed a malignant tumor with prominent keratinization, confirming the diagnosis of an invasive primary pure SCC of the gallbladder. Microscopic examination showed direct infiltration to the liver, duodenum, and stomach. This case report describes the hospital course of a patient with SCC of the gallbladder and suggests that gallbladder cancer should be considered as part of the differential diagnosis in elderly patients presenting with acute cholecystitis. In addition, this article will review existing literature to examine the utility of different diagnostic techniques and treatment modalities available in the management of gallbladder cancer.

  17. Digital next-generation sequencing identifies low-abundance mutations in pancreatic juice samples collected from the duodenum of patients with pancreatic cancer and intraductal papillary mucinous neoplasms.

    Science.gov (United States)

    Yu, Jun; Sadakari, Yoshihiko; Shindo, Koji; Suenaga, Masaya; Brant, Aaron; Almario, Jose Alejandro Navarro; Borges, Michael; Barkley, Thomas; Fesharakizadeh, Shahriar; Ford, Madeline; Hruban, Ralph H; Shin, Eun Ji; Lennon, Anne Marie; Canto, Marcia Irene; Goggins, Michael

    2017-09-01

    Secretin-stimulated pancreatic juice contains DNA shed from cells lining the pancreatic ducts. Genetic analysis of this fluid may form a test to detect pancreatic ductal neoplasia. We employed digital next-generation sequencing ('digital NGS') to detect low-abundance mutations in secretin-stimulated juice samples collected from the duodenum of subjects enrolled in Cancer of the Pancreas Screening studies at Johns Hopkins Hospital. For each juice sample, digital NGS necessitated 96 NGS reactions sequencing nine genes. The study population included 115 subjects (53 discovery, 62 validation) (1) with pancreatic ductal adenocarcinoma (PDAC), (2) intraductal papillary mucinous neoplasm (IPMN), (3) controls with non-suspicious pancreata. Cases with PDAC and IPMN were more likely to have mutant DNA detected in pancreatic juice than controls (both pcancer despite close surveillance had SMAD4/TP53 mutations from their cancer detected in juice samples collected over 1 year prior to their pancreatic cancer diagnosis when no suspicious pancreatic lesions were detected by imaging. The detection in pancreatic juice of mutations important for the progression of low-grade dysplasia to high-grade dysplasia and invasive pancreatic cancer may improve the management of patients undergoing pancreatic screening and surveillance. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

  18. Laparoscopy in repairing in 27 patients with gastric and duodenum perforation.%腹腔镜胃十二指肠穿孔修补27例体会

    Institute of Scientific and Technical Information of China (English)

    李军; 彭小妮; 崔海宁

    2011-01-01

    目的 总结腹腔镜胃、十二指肠穿孔修补经验.方法 对27例胃十二指肠穿孔患者施行腹腔镜修补术.结果 22例十二指肠穿孔、5例胃窦穿孔均行腹腔镜修补,平均住院5.2 d,无术中及术后并发症.结论 腹腔镜胃十二指肠穿孔修补术具有安全、可靠、患者恢复快和痛苦少等优点.对胃穿孔须考虑溃疡恶变或胃癌情况,术中须切取穿孔边缘组织行冰冻切片检查,以便及时发现胃癌,进行根治性治疗.%Objective To study the experience of laparoscopy in repairing in gastric and duodenum perforation. Methods 27 patients with gastric and duodenum perforation were performed hparoscopic repair. Results 22 patients with duodenum perforation and 5 patients with gastric perforation were safely operated. The mean time of hospitalization was 5.2days. There was no intraoperative and postoperative complication. Conclusion Laparoscopy is one of the safe, quick recovery and little pain in repairing duodenum perforation, but it should be done with great care in repairing gastric perforation for malignant change of gastric ulcer and primary gastric carcinoma. The perforated edge of frozen biopsy tissue should be preformed in order to discover gastric cancer and curative treatment.

  19. [The spa-and-health resort-based rehabilitation of the patients presenting with frequently recurring erosive and ulcerative lesions in the oesophagus, stomach, and duodenum in the phase of subsiding exacerbation].

    Science.gov (United States)

    Efimenko, N V; Kaĭsinova, A S

    2014-01-01

    To develop a new medical technology for the spa-and-health resort-based treatment of the patients presenting with frequently recurring erosive and ulcerative lesions in the oesophagus, stomach, and duodenum in the phase of subsiding exacerbation. A total of 100 patients presenting with frequently recurring erosive and ulcerative lesions in the oesophagus, stomach, and duodenum in the phase of subsiding exacerbation were available for the examination that included detailed investigations of the clinical picture, characteristics of the immune status, and psychological testing before and after a course of the spa-and-health resort-based treatment. The effectiveness of two therapeutic modalities was evaluated. One of them (control) prescribed to 50 patients consisted of standard antiulcer pharmacotherapy in combination with Essentuki Novaya drinking mineral water and carbon dioxide mineral baths, the other given to 50 patients included radon baths instead of carbon dioxide mineral baths. The combined application of drinking mineral waters, radon baths, and standard antiulcer pharmacotherapy produced the most conspicuous clinical effect in the framework of spa-and-health resort-based rehabilitation of the patients presenting with frequently recurring erosive and ulcerative lesions in the oesophagus, stomach, and duodenum in the phase of subsiding exacerbation as confirmed by positive dynamics of their psychoemotional status in 97.7% of the cases, regression of pain syndrome (91.5%), and improvement of humoral and cellular immunity (94%). An important result of the spa-and-health resort-based rehabilitation is the intensification of the reparative processes in gastroduodenal mucosa responsible for the healing of ulcers and erosions in 94.7% of the patients. The spa-and-health resort-based rehabilitation is a pathogenetically sound and efficacious approach to the management of the patients presenting with frequently recurring erosive and ulcerative lesions in the

  20. Unusual Development of Iatrogenic Complex, Mixed Biliary and Duodenal Fistulas Complicating Roux-en-Y Antrectomy for Stenotic Peptic Disease of the Supraampullary Duodenum Requiring Whipple Procedure: An Uncommon Clinical Dilemma

    OpenAIRE

    Polistina, Francesco A.; Giorgio Costantin; Alessandro Settin; Franco Lumachi; Giovanni Ambrosino

    2010-01-01

    Complex fistulas of the duodenum and biliary tree are severe complications of gastric surgery. The association of duodenal and major biliary fistulas occurs rarely and is a major challenge for treatment. They may occur during virtually any kind of operation, but they are more frequent in cases complicated by the presence of difficult duodenal ulcers or cancer, with a mortality rate of up to 35%. Options for treatment are many and range from simple drainage to extended resections and difficult...

  1. 十二指肠癌及溃疡组织幽门螺杆菌感染对比%Comparison of Helicobacter pylori infection between duodenum carcinoma and ulcer

    Institute of Scientific and Technical Information of China (English)

    李静; 刘岩

    2012-01-01

    Objective To investigate the expression and significance of Helicobacter pylori (Hp) in human duodenum carcinoma and ulcer. Methods The expression of Hp in 40 specimens of duodenal ulcer tissue ( control group) , 32 specimens of duodenum carcinoma tissue (observation group) was determined by immunohistochemjstry. Results The expression of Hp in control group (72. 5% ) was significantly higher than that ( 12. 5% ) in observation group (P <0. 005). Conclusions Hp infection may play an important role in the development of duodenal ulcer, not be relevant to the development of duodenum carcinoma.%目的 研究幽门螺杆菌( Helicobacter pylori,Hp)在十二指肠溃疡及十二指肠癌中的表达及意义.方法 采用免疫组织化学染色方法检测40例十二指肠溃疡组织(对照组)和32例十二指肠癌组织(观察组)中Hp表达情况.结果 对照组Hp阳性表达率(72.5%)与观察组(12.5%)差异显著(P<0.005).结论 Hp感染在十二指肠溃疡发生发展中起着重要作用,而与十二指肠癌发生关系不大.

  2. A meta-analysis of the long-term effects of chronic pancreatitis surgical treatments: duodenum-preserving pancreatic head resection versus pancreatoduodenectomy

    Institute of Scientific and Technical Information of China (English)

    L(U) Wen-ping; SHI Qing; ZHANG Wen-zhi; CAI Shou-wang; JIANG Kai; DONG Jia-hong

    2013-01-01

    Background Surgery is regarded as the most effective treatment to relieve pain and reduce complications in chronic pancreatitis (CP).Two major strategies exist:duodenum-preserving pancreatic head resection (DPPHR) and pancreatoduodenectomy (PD).Many studies suggest that DPPHR offers advantages during surgery and in the short-term; however,the long-term effects have not been thoroughly investigated.We analyzed the long-term outcomes of DPPHR and PD,over follow-up times of at least 1 year,to determine the optimal surgical treatment for CP.Methods We systemically reviewed all CP surgical treatment reports,and only included randomized controlled trials (RCT) comparing DPPHR and PD,excluding unqualified studies using several pre-specified criteria.When multiple publications of a single trial were found,the most comprehensive current data were selected.Characteristics of the study populations and long-term postoperative outcome parameters were collected.The quality of the studies and data was analyzed using RevMan 4.2 software.Results Five trials were qualified for meta-analysis,with 261 participants in total (114 in the DPPHR group and 147 in the PD group).There were no significant differences in the age,gender,or indications for surgery of each group.At the mean of 5.7-year (1-14 years) follow-up examination,DPPHR and PD resulted in equally effective pain relief,exocrine and endocrine function,and similar mortality rates (P >0.05); however,DPPHR patients had improved global quality of life and weight gain,and reduced diarrhea and fatigue (P <0.05).Conclusion DPPHR and PD result in equal pain relief,mortality,and pancreatic function; however,DPPHR provides superior long-term outcomes.

  3. Effect of surfactants, gastric emptying, and dosage form on supersaturation of dipyridamole in an in vitro model simulating the stomach and duodenum.

    Science.gov (United States)

    Mitra, A; Fadda, H M

    2014-08-04

    The purpose of this study was to investigate the influence of gastric emptying patterns, surfactants, and dosage form on the supersaturation of a poorly soluble weakly basic drug, dipyridamole, using an in vitro model mimicking the dynamic environment of the upper gastrointestinal tract, and, furthermore, to evaluate the usefulness of this model in establishing correlations to in vivo bioavailability for drugs with solubility/dissolution limited absorption. A simulated stomach duodenum model comprising four compartments was used to assess supersaturation and precipitation kinetics as a function of time. It integrates physiologically relevant fluid volumes, fluid transfer rates, and pH changes of the upper GI tract. Monoexponential gastric emptying patterns simulating the fasted state were compared to linear gastric emptying patterns simulating the fed state. The effect of different surfactants commonly used in oral preparations, specifically, sodium lauryl sulfate (SLS), poloxamer-188, and polysorbate-80, on dipyridamole supersaturation was investigated while maintaining surface tension of the simulated gastric fluids at physiological levels and without obtaining artificial micellar solubilization of the drug. The supersaturation behavior of different dose strengths of dipyridamole was explored. Significant levels of dipyridamole supersaturation were observed in the duodenal compartment under all the different in vivo relevant conditions explored. Dipyridamole supersaturation ratios of up to 11-fold have been observed, and supersaturation has been maintained for up to 120 min. Lower duodenal concentrations of dipyridamole were observed under linear gastric emptying patterns compared to mononexponential gastric emptying. The mean duodenal area under concentration-time curves (AUC60min) for the dipyridamole concentration profile in the duodenal compartment is significantly different for all the surfactants explored (P supersaturation/precipitation kinetics of weakly

  4. Long-term prolactin exposure differentially stimulated the transcellular and solvent drag-induced calcium transport in the duodenum of ovariectomized rats.

    Science.gov (United States)

    Tudpor, Kukiat; Charoenphandhu, Narattaphol; Saengamnart, Wasana; Krishnamra, Nateetip

    2005-12-01

    Prolactin, having been shown to stimulate transcellular active and solvent drag-induced calcium transport in the duodenum of female rats, was postulated to improve duodenal calcium transport in estrogen-deficient rats. The aim of the present study was, therefore, to demonstrate the effects of long-term prolactin exposure produced by anterior pituitary (AP) transplantation on the duodenal calcium transport in young (9-week-old) and adult (22-week-old) ovariectomized rats. We found that ovariectomy did not alter the transcellular active duodenal calcium transport in young and adult rats fed normal calcium diet (1.0% w/w Ca) but decreased the solvent drag-induced duodenal calcium transport from 75.50 +/- 10.12 to 55.75 +/- 4.77 nmol.hr(-1).cm(-2) (P calcium transport in young and adult AP-grafted ovariectomized rats fed with normal calcium diet by more than 2-fold from 7.56 +/- 0.79 to 16.54 +/- 2.05 (P calcium transport in young rats was enhanced by prolactin from 95.51 +/- 10.64 to 163.20 +/- 18.03 nmol.hr(-1).cm(-2) (P calcium supplement has been widely used to improve calcium balance in estrogen-deficient animals, the effect of a high-calcium diet (2.0% w/w Ca) was also investigated. The results showed that stimulatory action of long-term prolactin on the transcellular active duodenal calcium transport in both young and adult rats was diminished after being fed a high-calcium diet. The same diet also abolished prolactin-enhanced solvent drag-induced duodenal calcium transport in young and further decreased that in adult AP-grafted ovariectomized rats. We concluded that the solvent drag-induced duodenal calcium transport in adult rats was decreased after ovariectomy. Long-term prolactin exposure stimulated the transcellular active duodenal calcium transport in both young and adult rats whereas enhancing the solvent drag-induced duodenal calcium transport only in young rats. Effects of prolactin were abolished by a high-calcium diet.

  5. Cysteamine-colon and cysteamine-duodenum lesions in rats. Attenuation by gastric pentadecapeptide BPC 157, cimetidine, ranitidine, atropine, omeprazole, sulphasalazine and methylprednisolone.

    Science.gov (United States)

    Sikiric, P; Seiwerth, S; Grabarevic, Z; Balen, I; Aralica, G; Gjurasin, M; Komericki, L; Perovic, D; Ziger, T; Anic, T; Prkacin, I; Separovic, J; Stancic-Rokotov, D; Lovric-Bencic, M; Mikus, D; Staresinic, M; Aralica, J; DiBiaggio, N; Simec, Z; Turkovic, B; Rotkvic, I; Mise, S; Rucman, R; Petek, M; Sebecic, B; Ivasovic, Z; Boban-Blagaic, A; Sjekavica, I

    2001-01-01

    also attenuated by these agents application (given in the same doses, i.p., 1 h before cysteamine), with only exception to sulphasalazine. Thus, the extended cysteamine specific ulcerogenic effect, cysteamine colon/duodenum lesion-link and an extenuation of agents protection from upper to lower part of gastrointestinal tract (i.e. stomach pentadecapeptide BPC 157, standard antiulcer agents, cimetidine, ranitidine, atropine, omeprazole) and vice versa (remedies for inflammatory bowel disease) evidenced in the present study may be potentially important for both further experimental and clinical research.

  6. Therapeutic ERCP strategies in patients with duodenum stricture%合并十二指肠狭窄患者实施经内镜逆行胰胆管造影术的策略

    Institute of Scientific and Technical Information of China (English)

    潘亚敏; 吴军; 王田田; 高道健; 胡冰

    2012-01-01

    目的 探讨合并十二指肠狭窄患者实施ERCP诊疗的方法与策略.方法 回顾性分析合并十二指肠狭窄患者的ERCP处理方法,观察内镜操作的成功率及并发症.结果 7276例接受ERCP的患者中,合并十二指肠狭窄158例(2.17%),其中恶性肿瘤浸润肠壁120例,十二指肠良性狭窄38例.采用改变体位或导丝引导的方法顺利通过狭窄处完成ERCP及胆道引流96例,实施柱状水囊扩张术后完成ERCP 33例,先放置十二指肠金属支架后行胆管引流4例,另有25例未能完成或放弃治疗.内镜操作成功率84.2% (133/158),术中有少量出血5例,未发生持续出血或穿孔等并发症.结论 十二指肠狭窄患者仍有可能完成ERCP操作,轻度狭窄可通过改变体位或导丝引导帮助内镜通过,重度狭窄需采用水囊扩张或先留置肠道支架,根据局部病变情况循序应用以上方法实施内镜治疗仍是安全的,且成功率较高.%Objective To investigate the methods and strategies of therapeutic ERCP in patients with duodenum stricture.Methods Endoscopic procedure,success rate and complications in patients with duodenum stricture who underwent ERCP were retrospectively analyzed.Results In 7276 patients who underwent therapeutic ERCP,duodenum stricture was found in 158 (2.17%),patients with malignant tumor infiltration in 120 and benign stricture in 38.The total success rate of ERCP and biliary drainage was 84.2%,with posture change or guidewire leading method in 96 patients,stylolitic water sac dilation in 33and duodenum metal stent placement before biliary drainage in 4.The procedure was failed in 25 patients.Minor bleeding occurred in 5 patients and no major complication including massive bleeding or perforation was observed.Conclusion ERCP is safe,effective and feasible for patients with duodenum stricture,which can be performed by posture change or guidewire leading method in mild stricture,and stylolitic water sac dilation or

  7. 原发性十二指肠梭形细胞鳞癌的组织形态学及蛋白表达的研究%Histomorphology and protein expression in primary duodenum spindle cell squamous carcinoma

    Institute of Scientific and Technical Information of China (English)

    周英泽; 罗红波; 王锡平; 王桂芬

    2014-01-01

    Objective To explore the histomorphology and protein expression characteristics of the primary duodenum spindle cell squamous carcinoma, and determine its histological origin, and provide comprehensive under-standing in the awareness and diagnosis of this disease. Methods HE staining was used to understand the histomor-phology features of the primary duodenum spindle cell squamous carcinoma, and immunohistochemistry staining was applied to detect the protein expression. Results The HE staining showed pleomorphism fusiform tumor cells in the mucosa, submucosa, and muscular, with a few atypical intercellularbridge and keratin pearl. And the immunohisto-chemical staining revealed fusiform tumor cell area as follows:(1) staining in cytoplasm:CK5/6(3+), CEA(3+), CK8/18 (2+), Syn (+), NSE(+), CgA(+) and S-100 protein(+);(2) staining in nuclei:Ki-67 (3+);(3) negative in CD34 and CD117. Conclusion The primary duodenum spindle cell squamous carcinoma arised from the multipotent stem cell in the basal layer of the mucosa duodenum.%目的:探讨十二指肠梭形细胞鳞癌的组织形态学及蛋白表达的特点,明确其组织学来源,以提高对该疾病的认识和诊断水平。方法运用HE染色法了解十二指肠梭形细胞鳞癌的组织形态学特点;通过免疫组织化学染色法进一步了解其蛋白表达特点。结果 HE染色显示黏膜层、黏膜下层及肌层见大小不等异型性明显的梭形肿瘤细胞,并见部分不典型细胞间桥和角化珠形成。免疫组织化学染色结果显示梭形肿瘤细胞区域:⑴胞浆着色:CK5/6(+++)、CEA(+++)、CK8/18(++)、Syn(+)、NSE(+)、CgA(+)及S-100蛋白(+);⑵胞核着色:Ki-67(+++);⑶CD34和CD117显示阴性。结论十二指肠鳞状细胞癌起源于十二指肠黏膜基底层多潜能干细胞。

  8. Unusual Development of Iatrogenic Complex, Mixed Biliary and Duodenal Fistulas Complicating Roux-en-Y Antrectomy for Stenotic Peptic Disease of the Supraampullary Duodenum Requiring Whipple Procedure: An Uncommon Clinical Dilemma

    Directory of Open Access Journals (Sweden)

    Francesco A. Polistina

    2010-10-01

    Full Text Available Complex fistulas of the duodenum and biliary tree are severe complications of gastric surgery. The association of duodenal and major biliary fistulas occurs rarely and is a major challenge for treatment. They may occur during virtually any kind of operation, but they are more frequent in cases complicated by the presence of difficult duodenal ulcers or cancer, with a mortality rate of up to 35%. Options for treatment are many and range from simple drainage to extended resections and difficult reconstructions. Conservative treatment is the choice for well-drained fistulas, but some cases require reoperation. Very little is known about reoperation techniques and technical selection of the right patients. We present the case of a complex iatrogenic duodenal and biliary fistula. A 42-year-old Caucasian man with a diagnosis of postoperative peritonitis had been operated on 3 days earlier; an antrectomy with a Roux-en-Y reconstruction for stenotic peptic disease was performed. Conservative treatment was attempted with mixed results. Two more operations were required to achieve a definitive resolution of the fistula and related local complications. The decision was made to perform a pancreatoduodenectomy with subsequent reconstruction on a double jejunal loop. The patient did well and was discharged on postoperative day 17. In our experience pancreaticoduodenectomy may be an effective treatment of refractory and complex iatrogenic fistulas involving both the duodenum and the biliary tree.

  9. Unusual Development of Iatrogenic Complex, Mixed Biliary and Duodenal Fistulas Complicating Roux-en-Y Antrectomy for Stenotic Peptic Disease of the Supraampullary Duodenum Requiring Whipple Procedure: An Uncommon Clinical Dilemma

    Science.gov (United States)

    Polistina, Francesco A.; Costantin, Giorgio; Settin, Alessandro; Lumachi, Franco; Ambrosino, Giovanni

    2010-01-01

    Complex fistulas of the duodenum and biliary tree are severe complications of gastric surgery. The association of duodenal and major biliary fistulas occurs rarely and is a major challenge for treatment. They may occur during virtually any kind of operation, but they are more frequent in cases complicated by the presence of difficult duodenal ulcers or cancer, with a mortality rate of up to 35%. Options for treatment are many and range from simple drainage to extended resections and difficult reconstructions. Conservative treatment is the choice for well-drained fistulas, but some cases require reoperation. Very little is known about reoperation techniques and technical selection of the right patients. We present the case of a complex iatrogenic duodenal and biliary fistula. A 42-year-old Caucasian man with a diagnosis of postoperative peritonitis had been operated on 3 days earlier; an antrectomy with a Roux-en-Y reconstruction for stenotic peptic disease was performed. Conservative treatment was attempted with mixed results. Two more operations were required to achieve a definitive resolution of the fistula and related local complications. The decision was made to perform a pancreatoduodenectomy with subsequent reconstruction on a double jejunal loop. The patient did well and was discharged on postoperative day 17. In our experience pancreaticoduodenectomy may be an effective treatment of refractory and complex iatrogenic fistulas involving both the duodenum and the biliary tree. PMID:21103208

  10. High-level recombinant human lysozyme expressed in milk of transgenic pigs can inhibit the growth of Escherichia coli in the duodenum and influence intestinal morphology of sucking pigs.

    Directory of Open Access Journals (Sweden)

    Dan Lu

    Full Text Available Lysozyme is often used as a feed additive and acts as an antimicrobial protein that enhances immune function and defends against pathogenic bacteria in pigs. In this study, we genetically added recombinant human lysozyme (rhLZ to sow milk by somatic cell nuclear transfer and investigated whether the presence of recombinant human lysozyme can influence intestinal microbiota and morphology in sucking pigs. We generated transgenic cloned pigs and the first-generation hybrids (F1 produced high levels of rhLZ in milk. The average concentration of rhLZ was 116.34 ± 24.46 mg/L in the milk of F1 sows, which was 1500-fold higher than that of the native pig lysozyme. In vitro, it was demonstrated that rhLZ in milk of transgenic pigs had enzyme levels at 92,272 ± 26,413 U/mL. In a feeding experiment, a total of 40 newborn piglets were nursed by four transgenic sows and four sibling non-transgenic sows (F1, with five piglets per gilt. The piglets were allowed to nurse for 21 days and the sow milk was the only source of nutrition for the piglets. All piglets were slaughtered on postnatal day 22. Six types of bacteria were cultured and analyzed to detect the impact of rhLZ on gut microbiota. The number of Escherichia coli in the duodenum of piglets reared by transgenic sows was significantly decreased (p<0.001 and their villus height to crypt depth ratio in the intestine were increased due to the significant decrease of crypt depth in the duodenum, jejunum, and ileum (p<0.001. Together, we successfully generated rhLZ transgenic cloned pigs and elevated lysozyme level in nuring piglets. The results of the feeding experiments demonstrated that rhLZ-enhanced milk can inhibit the growth of E. coli in the duodenum and positively influence intestinal morphology without adversely affecting weight gain or piglet growth.

  11. Antacid activity of calcium carbonate and hydrotalcite tablets. Comparison between in vitro evaluation using the "artificial stomach-duodenum" model and in vivo pH-metry in healthy volunteers.

    Science.gov (United States)

    Vatier, J; Ramdani, A; Vitré, M T; Mignon, M

    1994-04-01

    Antacid activity of calcium carbonate (CAM, Rennie) and of hydrotalcite (HYD) containing tablets has been assessed in vitro using computer-controlled "artificial stomach-duodenum" model, including or not a piece of hog gastric mucosa in the gastric reservoir, and simulating the constant flux system or the normal gastroduodenal flux regulation. The data obtained under the latter condition were compared to those obtained in vivo by pH-metry in 12 healthy volunteers, in response to one administration of 2 tablets. The theoretical maximal capacity was similar when 1 tablet of CAM or of HYD was added to the gastric contents, including or not a piece of gastric mucosa, close to 95 H+ mmol. The reduction of acid load penetrating into the duodenum and the duodenal pH were of the same magnitude in response to both antacids. When normal gastroduodenal flux regulation was simulated, a dose-response curve, constructed by 1, 2 or 3 tablets, resulted in the same antacid characteristics in response to both antacids. The maximal gastric and the mean duodenal pH values obtained with CAM were, however, higher than with HYD, corresponding to a greater neutralizing activity developed by CAM than by HYD. The comparison between in vivo administration of two tablets of each antacid and the in vitro model simulating normal gastroduodenal flux regulation in response to the addition of two tablets resulted in similar data. The maximal pH values obtained in in vivo assays were slightly lower than in vitro, depending on the ratio of antacid amount to gastric acid content, well established in in vitro conditions and unknown in in vivo situations.(ABSTRACT TRUNCATED AT 250 WORDS)

  12. High-level recombinant human lysozyme expressed in milk of transgenic pigs can inhibit the growth of Escherichia coli in the duodenum and influence intestinal morphology of sucking pigs.

    Science.gov (United States)

    Lu, Dan; Li, Qiuyan; Wu, Zhibin; Shang, Shengzhe; Liu, Shen; Wen, Xiao; Li, Zhiyuan; Wu, Fangfang; Li, Ning

    2014-01-01

    Lysozyme is often used as a feed additive and acts as an antimicrobial protein that enhances immune function and defends against pathogenic bacteria in pigs. In this study, we genetically added recombinant human lysozyme (rhLZ) to sow milk by somatic cell nuclear transfer and investigated whether the presence of recombinant human lysozyme can influence intestinal microbiota and morphology in sucking pigs. We generated transgenic cloned pigs and the first-generation hybrids (F1) produced high levels of rhLZ in milk. The average concentration of rhLZ was 116.34 ± 24.46 mg/L in the milk of F1 sows, which was 1500-fold higher than that of the native pig lysozyme. In vitro, it was demonstrated that rhLZ in milk of transgenic pigs had enzyme levels at 92,272 ± 26,413 U/mL. In a feeding experiment, a total of 40 newborn piglets were nursed by four transgenic sows and four sibling non-transgenic sows (F1), with five piglets per gilt. The piglets were allowed to nurse for 21 days and the sow milk was the only source of nutrition for the piglets. All piglets were slaughtered on postnatal day 22. Six types of bacteria were cultured and analyzed to detect the impact of rhLZ on gut microbiota. The number of Escherichia coli in the duodenum of piglets reared by transgenic sows was significantly decreased (ptransgenic cloned pigs and elevated lysozyme level in nuring piglets. The results of the feeding experiments demonstrated that rhLZ-enhanced milk can inhibit the growth of E. coli in the duodenum and positively influence intestinal morphology without adversely affecting weight gain or piglet growth.

  13. Modulator Effects of Astragals Polysaccharides on Duodenum Mucosal Immunity of Broilers%黄芪多糖对肉仔鸡十二指肠黏膜的免疫调节作用

    Institute of Scientific and Technical Information of China (English)

    王海凤; 郭兵

    2015-01-01

    本试验研究了黄芪多糖( APS)对肉仔鸡十二指肠黏膜的免疫调节作用. 200只1日龄爱拔益加( AA)肉鸡随机分成4组,每组50只,Ⅰ组为对照组,Ⅱ、Ⅲ、Ⅳ组为试验组. Ⅱ、Ⅲ、Ⅳ组于5~7日龄分别经口注入3种不同浓度(6、4、2 mg/mL)APS,0.5 mL/只,1次/d,连用3 d,对照组灌服等量生理盐水. 7 日龄,每只采用鸡新支二联苗2 倍量点眼滴鼻免疫. 分别于8、15、22日龄取样,测定各组10只肉仔鸡十二指肠黏膜免疫球蛋白含量、白细胞介素-2( IL?2)含量和黏膜免疫相关细胞数量. 结果显示:高、中剂量APS可显著提高15日龄肉仔鸡十二指肠黏膜免疫球蛋白A、免疫球蛋白G、免疫球蛋白M含量( P<0.05) ,高、中剂量APS可显著或极显著提高15、22日龄肉仔鸡十二指肠黏膜 IL?2含量和上皮内淋巴细胞、肥大细胞、杯状细胞数量( P<0.05或P<0.01). 由此可见,APS可增强肉仔鸡十二指肠黏膜免疫功能,以6、4 mg/mL剂量最佳.%This experiment was conducted to investigate the modulator effects of astragals polysaccharides ( APS) on duodenum mucosal immunity of broilers. Two hundred Arbor Acres broilers were randomly divided into four groups of 50 broilers per group: group Ⅰ was control group, while the other 3 groups were experi?mental groups which broilers were orally administered with 0.5 mL APS at 3 different concentrations (6, 4, 2 mg/mL) once per day for three days, and broilers in groupⅠwere oral administratied the same volume of sa?line . All broilers at 7 days of age were vaccined with combined vaccine against Newcastle disease and infectious bronchitis with 2 times the volume by drop and nasal immunization. Duodenum mucosa was sampled on 8, 15, 22 days of age with ten broilers for detecting contents of immunoglobulin and interleukin?2 ( IL?2) , and for measurement of the numbers of mucosal immunity?associated cells, respectively. The results showed as fol?lows: contents of immunoglobulin

  14. Modified duodenum-preserving pancreatic head resection in treatment of patients with chronic pancreatitis%改良保留十二指肠胰头切除术治疗慢性胰腺炎的疗效分析

    Institute of Scientific and Technical Information of China (English)

    魏洪吉; 吴河水; 熊炯炘; 陶京; 周峰; 王春友

    2011-01-01

    目的:观察改良保留十二指肠的胰头切除术(改良Beger手术)对伴胰头炎性肿块的慢性胰腺炎病人的治疗效果.方法:回顾性分析自2004年1月至2010年12月,在我院胰腺外科接受改良Beger手术治疗的51例伴胰头炎性肿块的慢性胰腺炎病人的临床资料,并对病人术后疼痛症状、生活质量及内分泌功能等进行随访.结果:无手术死亡病例,术后并发症发生率为15.7%,其中胰漏3例,胆漏2例,十二指肠漏l例,腹腔出血1例,切口裂开1例.术后6个月,病人疼痛得到明显缓解,EORTC QLQ-C30疼痛评分由(64.3±5.8)降至(12.5±3.7)(P<0.01),生活质量获显著提高,GLQI生活质量评分由(70.1±5.8)增至(86.4±6.6)(P<0.01);病人内分泌功能未受影响,无新增糖尿病病例.结论:采用改良Beger手术治疗伴胰头炎性肿块的慢性胰腺炎是安全、有效的.%Objective To observe the therapeutic efficacy of a modified duodenum-preserving pancreatic head resection procedure in the treatment of patients with chronic pancreatitis and an inflammatory mass in the head of the pancreas. Methods From Jan 2004 to Dec 2010, a modified duodenum-preserving pancreatic head resection procedure was performed in fifty-one patients with chronic pancreatitis and an inflammatory mass in the head of the pancreas. The pain scale in the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30), Gastrointestinal Quality of Life Index (GLQI) questionnaire, and oral glucose tolerance test (OGTT) were used to evaluate the changes in preoperative and postoperative pain symptomatology, quality of life, and endocrine function of the patients correspondingly. Results There was no hospital mortality. The overall postoperative incidence rate of complications was 13.7%, including pancreatic fistula in 3 cases, bile leakage in 2 cases, duodenal fistula in 1 case, intraabdominal bleeding in 1 case and wound disruption in 1 case

  15. Ultrastructural analysis of kidney, liver and duodenum isolated from treated rats with Ginkgo Biloba extract and effects of this medicinal plant on the biodistribution of the radiopharmaceutical sodium pertechnetate

    Energy Technology Data Exchange (ETDEWEB)

    Moreno, Silvana Ramos Farias; Arnobio, Adriano; Olej, Beni; Timoteo, Margareth de Oliveira; Caldas, Luiz Querino de Araujo [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil)]. E-mail: srfmoreno@hotmail.com; Carvalho, Jorge Jose de; Nascimento, Ana Lucia [Universidade do Estado do Rio de Janeiro (UERJ), RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes. Dept. de Histologia e Embriologia; Bernardo Filho, Mario [Universidade do Estado do Rio de Janeiro (UERJ), RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes. Dept. de Biofisica e Biometria

    2008-12-15

    Ginkgo biloba extract (EGb) has been used to treat memory and concentration deficits, acts as platelet activating factor antagonism and prevents against damages caused by free radicals. EGb is a standardized extract that contains 24% flavonoids and 6% terpenoids. The aim of this work was to evaluate the possible influence of an EGb on the ultrastructure of some organs isolated from rats and on the biodistribution of sodium pertechnetate ({sup 99m}TcO{sub 4}Na). The animals were treated with EGb and after six days, received {sup 99m}TcO{sub 4}Na. The organs were isolated and fixed for ultrastructural analysis. The results showed that EGb has modified the ultrastructure of kidney, liver and duodenum and altered the biodistribution of {sup 99m}TcO{sub 4}Na (P<0.05). It is speculated that the substances present in the EGb could act directly or generate metabolites capable to promote changes on the biodistribution of {sup 99m}TcO{sub 4}Na and on the morphology of organs at ultrastructural level. (author)

  16. The mRNA and Protein Levels of Tubulin and β-Actin Are Greatly Reduced in the Proximal Duodenum of Mice Relative to the Rest of the Small Intestines.

    Science.gov (United States)

    Yu, Sungsook; Hwang, Hyekyung E; Yun, Nakhyeon; Goldenring, James R; Nam, Ki Taek

    2015-09-01

    To accurately quantify mRNA and protein levels, it is critical to choose appropriate internal standards. As the expression of housekeeping genes is assumed to remain constant, they are often employed to normalize signals to correct for sample-to-sample variations. However, recent studies have documented that β-actin and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression levels change in response to various stimuli during proliferation, activation, and differentiation. We investigated levels of α-, β-, γ-tubulin, β-actin, and GAPDH vary across the gastrointestinal tract of mice. We found that different regions of the small intestines had dramatically different expression profiles, as measured by western blot, quantitative Reverse transcription polymerase chain reaction (RT-PCR), and immunohistochemical staining. These results revealed that the expression levels of tubulins and β-actin were dramatically lower in the proximal duodenum, relative to the rest of the small intestines. These varying levels of housekeeping genes may reflect differences in the activities of specialized tissues and suggest unique requirements for tubulins in these tissue types. We conclude that the use of a single housekeeping gene to normalize gene expression in the gastrointestinal tracts of mice may introduce errors, as measured differences in gene expression may reflect regulation of the internal control rather than the mRNA or protein under investigation.

  17. [A Case of Von Hippel-Lindau Disease with Nonfunctioning Pancreatic Neuroendocrine Tumors Treated by Duodenum-Preserving Resection of the Head of the Pancreas and Spleen-Preserving Resection of the Tail of the Pancreas].

    Science.gov (United States)

    Umehara, Yutaka; Umehara, Minoru; Tokura, Tomohisa; Yachi, Takafumi; Takahashi, Kenichi; Morita, Takayuki; Hakamada, Kenichi

    2015-10-01

    A 26-year-old woman presented to our department with a diagnosis of multiple nonfunctioning pancreatic neuroendocrine tumors. She had a family history of pheochromocytoma and a medical history of bilateral adrenalectomy for pheochromocytoma at the age of 25 years. During follow-up treatment for adrenal insufficiency after the surgery, highly enhanced tumors in the pancreas were detected on contrast-enhanced CT. Other examinations found that the patient did not satisfy the clinical criteria for von Hippel-Lindau (VHL) disease. Considering her age and risk of developing multiple heterotopic and heterochronous tumors, we performed a duodenum-preserving resection of the head of the pancreas and spleen-preserving resection of the tail of the pancreas with informed consent. The histopathological findings revealed that all of the tumors were NET G1. She underwent genetic testing postoperatively and was diagnosed with VHL disease. This diagnosis meant that we were able to create an optimal treatment plan for the patient. If a tumor predisposition syndrome is suspected, VHL disease should be borne in mind and genetic testing after genetic counseling should be duly considered.

  18. Prevention and treatment of postoperative complications of duodenum preserving pancreatic head resection%保留十二指肠胰头切除术后并发症的防治体会

    Institute of Scientific and Technical Information of China (English)

    朱世凯; 吴河水; 周玉; 熊炯圻; 赵刚; 王春友

    2009-01-01

    Objective To investigate the approach for prevention and treatment of postoperative complications of the duodenum-preserving pancreatic head resection (DPPHR). Method The clinical data of 56 patients receiving DPPHR in our hospital from 2003 to 2008 were retrospectively analyzed. Results Thirteen (23.3%) of the 56 patients had complications after DPPHR. The complications were pancreatic fistula in 7 cases (12.5%), duodenal fistula in 2 (5.4%) and biliary fistula in 1 (2.8%) and they were healed by supportive treatment and unobstructed drainage. Three cases (2.2%) of retroperitoneal infection were treated by putting drainage tube under ultrasonic guidance, 1 case (2.8%) with abdominal hemorrhage was disclosed to rupture and bleed in the branch of gastro-duodenal artery by selective celiac arteriography on emergency, and then treated with embolism with gelatin sponge and stainless steel. Conclusion The main complications after DPPHR are pancreatic fistula, duodenal fistula, biliary fistula, abdominal infection and hemorrhage. Meticulous operative technique, strict prehension of operation indications and complete reservation of blood supply of duo-denum are the key points to reduce postoperative complications and morbidity and increase operative a-chievement ratio after DPPHR. Meanwhile, correct methods should be used upon finding the compli-cations.%目的 探讨保留十二指肠胰头切除术后并发症的防治措施.方法 回顾性分析2003-2008年期间武汉协和医院胰腺中心行保留十二指肠胰头切除术56例病人的临床诊疗经过.结果 术后发生并发症13例(23.2%),包括胰瘘7例(12.5%),十二指肠瘘2例(5.4%);胆瘘1例(2.8%);腹膜后积液和感染2例(5.4%);腹腔大出血1例(2.8%).消化道瘘经支持治疗和维持通畅引流等治疗而痊愈,腹膜后积液和感染病人在B超引导下置管引流治愈,腹腔大出血者急诊选择性腹腔动脉造影显示胃十二指肠动脉分支破裂出血,经明胶海绵

  19. 12.2.Stomach and duodenum

    Institute of Scientific and Technical Information of China (English)

    1992-01-01

    920111 Inhibitory effects of enzymolyticproduct from normal human plasma onhematogenous metastrasis of murine gastro-carcinoma cells.JIAO Shunchang (焦顺昌),etal.General Hosp,PLA,Beijing.Natl Med J China1991:71 (10): 551-554.

  20. 12.2.Stomach and duodenum

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    930309 Evaluation of etiologic factors of gas-tric cancer in Tianjin Ⅱ.Relationship betweendiet and gastric cancer.REN Dalin (任大林),etal.Tianjin Med coll.Tianjin med J 1992;20(12):711-714.A case-control study involving interviewswith 97 gastric cancer patients and equal num-ber of matched controls from patients with otherthan cancer in a general hospital was conductedto investigate the dietary,nutrient and other fac-tors in the incidence of gastric cancer in Tianjin.Data were analysed by using conditional logisticregression model.The results showed that lowvegetable,low fruits,low potato and low vitaminC intakes,and unhealthy dietary habits,high salt

  1. Ultrastructural analysis of kidney, liver and duodenum isolated from treated rats with Ginkgo Biloba extract and effects of this medicinal plant on the biodistribution of the padiopharmaceutical sodium pertechnetate

    Directory of Open Access Journals (Sweden)

    Silvana Ramos Farias Moreno

    2008-12-01

    Full Text Available Ginkgo biloba extract (EGb has been used to treat memory and concentration deficits, acts as platelet activating factor antagonism and prevents against damages caused by free radicals. EGb is a standardized extract that contains 24% flavonoids and 6% terpenoids. The aim of this work was to evaluate the possible influence of an EGb on the ultrastructure of some organs isolated from rats and on the biodistribution of sodium pertechnetate (99mTcO4Na. The animals were treated with EGb and after six days, received 99mTcO4Na. The organs were isolated and fixed for ultrastructural analysis. The results showed that EGb has modified the ultrastructure of kidney, liver and duodenum and altered the biodistribution of 99mTcO4Na (PO extrato de Ginkgo Biloba extract (EGb tem sido usado para tratar dificuldades de concentração, memória, age como antagonista do fator de ativação de plaquetas e previne contra danos causados por radicais livres. EGb é um extrato padronizado com 24% de flavonóides e 6% de lactonas terpênicas. O objetivo deste estudo foi avaliar o efeito de um EGb na ultraestrutura de alguns órgãos isolados de ratos e na biodistribuição do radiofármaco pertecnetato de sódio (99mTcO4Na. Os animais foram tratados com EGb e após 6 dias receberam 99mTcO4Na. Os órgãos foram isolados e a radioatividade determinada. Os tecidos foram fixados para microscopia eletrônica. Os resultados mostraram que EGb alterou a ultraestrutura do rim, figado e duodeno e modificou a biodistribuição do 99mTcO4Na. Sugerimos que substâncias presentes no EGb poderiam agir diretamente ou gerar metabólitos capazes de promover alterações na biodistribuição e na morfologia de órgãos em nível ultraestrutural.

  2. Efficacy of modified duodenum-preserving pancreatic head resection for chronic pancreatitis%改良保留十二指肠胰头切除术治疗慢性胰腺炎的临床疗效

    Institute of Scientific and Technical Information of China (English)

    杨明; 赵刚; 吴河水; 王春友

    2014-01-01

    Objective To investigate the efficacy of a modified duodenum-preserving pancreatic head resection (DPPHR) for the treatment of chronic pancreatitis.Methods The clinical data of 109 patients with chronic pancreatitis who received modified DPPHR at the Union Hospital of Huazhong University of Science and Technology from January 2004 to June 2013 were retrospectively analyzed.Of the 109 patients,66 were with mass in the head of the pancreas,29 were with calcification of the head of the pancreas,14 were with atrophy of the head of the pancreas and stones in the main pancreatic duct.The level of glucose of 56 patients were normal,34 patients had glucose tolerance abnormalities and 19 were complicated with diabetes mellitus.Modified DPPHR was carried out after confirming the diagnosis of chronic pancreatitis and excluding the malignancies by frozen pathological examination.The head of the pancreas was completely resected.The posterior pancreaticoduodenal aortic arch running parallel to the duodenum was preserved to guarantee the blood supply to the remaining duodenum.A thin sheet of the pancreatic tissue behind the intrapancreatic common bile duct and between the common bile duct and the duodenum was preserved to guarantee the blood supply to the common bile duct.The gastrointestinal tract was reconstructed with an anastomosis of the distal pancreas and the jejunum and an end-to-en anastomosis of the proximal jejunum and the distal jejunum.Patients were followed up via out-patient examination to learn the frequency of abdominal pain,analgesics usage and the endocrine function.The pain scale,life quality and endocrine function were evaluated using the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30),Gastrointestinal Quality of Life Index (GLQI) questionnaire,and oral glucose tolerance test (OGTT),respectively.Patients were followed up till January 2014.The measurement data and the count data were analyzed using the t

  3. Number and size of myenteric neurons of the duodenum of adult rats with acute diabetes Número e tamanho dos neurônios mientéricos do duodeno de ratos adultos com diabetes agudo

    Directory of Open Access Journals (Sweden)

    MARIA MONTSERRAT D.P. FURLAN

    1999-09-01

    Full Text Available This study had as its purpose to assess the effects of acute diabetes induced by streptozotocin (35 mg/kg body weight on the number and size of the myenteric neurons of the duodenum of adult rats considering equally the antimesenteric and intermediate regions of the intestinal circumference. Experimental period extended for a week. Neuronal counts were carried out on the same number of fields of both regions of the duodenal circumference and measurements of neuronal and nuclear areas on equal numbers of cells. Number and size of the myenteric neurons stained with Giemsa were not significantly different between groups. On the other hand, the proportion of NADH-positive neurons increased from 18.54% on the controls to 39.33% on the diabetics. The authors discuss that this increased reactivity probably results from a greater NADH/NAD+ ratio, described in many tissues of diabetic animals, which has consequences on the modulation of the enzymes that use these cofactors and whose activity is detected by the NADH-diaphorase technique.Este estudo teve como objetivo avaliar os efeitos do diabetes agudo induzido por estreptozootocina (35 mg/kg de peso corporal sobre o número e tamanho dos neurônios mientéricos do duodeno de ratos adultos considerando de forma equivalente as regiões antimesentérica e intermediária da circunferência intestinal. O período experimental se estendeu por uma semana. As contagens neuronais foram feitas em igual número de campos nas duas regiões da circunferência duodenal e as mensurações das áreas neuronais e nucleares em igual número de células. O número e o tamanho dos neurônios corados por Giemsa não foram significativamente diferentes entre os grupos. Por outro lado, a proporção de neurônios NADH-positivos aumentou de 18,54% nos animais controles para 39,33% nos diabéticos. Os autores discutem que essa maior reatividade possivelmente resultou do aumento da proporção NADH/NAD+, descrita em diversos

  4. 64-slice spiral CT diagnosis of juxtapapillary diverticulum of duodenum%64层螺旋CT在十二指肠乳头旁憩室中的诊断

    Institute of Scientific and Technical Information of China (English)

    陈穹; 王钢; 汪茂文; 钱春锋; 姚强; 路明; 汤友英

    2011-01-01

    目的 探讨64层螺旋CT在十二指肠乳头旁憩室(JDD)中的诊断价值.方法 回顾分析经64层螺旋CT扫描发现并经消化道造影或内窥镜逆行性胆胰管造影(ERCP)证实的JDD 34例,通过后处理软件进行多平面重建(MPR)、容积再现(VR),显示其与胆总管下端的关系,并结合临床进行分析.结果 34例JDD,共发现乳头上型19例,乳头缘型10例,乳头水平外侧缘型1例,乳头下型4例.34例JDD合并胆总管结石7例,胆总管下端炎性狭窄11例,胆囊结石7例,急性胆囊炎4例,慢性胆囊炎17例,合并急性胰腺炎1例,慢性胰腺炎2例,合并肝内胆管小结石3例.结论 64层螺旋CT强大的后处理功能,不仅使JDD的检出率明显提高,更可显示其与十二指肠乳头的关系,可对临床胆系疾病的发病原因提供有益的帮助.%Objective To explore the value of 64-slice spiral CT in the diagnosis of juxtapapillary diverticulum of duodenum (JDD). Methods CT data in 34 cases of JDD confirmed by gastrointestinal radiography or endoscopic retrograde cholangiopancre-atography(ERCP) were retrospectively analysed. The relationship between the diverticulum and distal common bile duct was showed with multiplanar reconstruction!MPR) and volume rendering ( VR) by post-processing software. Results Among 34 cases, there were 19 cases of papillary-superior, 10 cases of nipple-margin, 1 case of the lateral opposite of the papillary-margin and 4 cases of papillary-inferior. In 34 cases ,there were 7 cases in combination with common bile duct stones, 11 cases of distal common bile duct inflammatory stricture,7 cases of cholelithiasis, 4 cases of acute cholecystitis, 17 cases of chronic cholecystitis, 1 case of acute pancreatitis, 2 cases of chronic pancreatitis and 3 cases of intrahepatic bile duct calculus correspondingly. Conclusion 64-slice spiral CT post-processing technology can not only improve the detecting rate of JDD significantly, but also can display the relationship

  5. 十二指肠灌注亮氨酸对山羊胰腺外分泌功能的影响%Infusion of Leucine into Duodenum Affects Pancreatic Exocrine Secretion of Goats

    Institute of Scientific and Technical Information of China (English)

    于红霞; 于志鹏; 刘凯; 姚军虎; 徐明

    2011-01-01

    本试验旨在研究十二指肠灌注亮氨酸( leucine,Leu)对山羊胰腺外分泌及血浆指标的影响,进而为提高反刍动物小肠淀粉利用率提供理论依据.本研究采用4×4拉丁方试验设计,选用4只周岁龄关中奶山羊,手术安装十二指肠瘘管、总胆管插管,进行十二指肠Leu灌注试验(灌注水平分别为0、3、6、9 g/d),研究Leu不同灌注水平对胰腺外分泌功能、血浆葡萄糖及胰岛素浓度的影响.结果表明:十二指肠灌注Leu,血浆中的葡萄糖和胰岛素含量、胰液分泌量、胰液总蛋白质浓度及合成速率、胰蛋白酶、糜蛋白酶和脂肪酶的分泌均不受影响(P>0.05).随Leu灌注水平的提高,胰腺α-淀粉酶的分泌浓度差异不显著(P>0.05),而分泌速率呈先升高后降低再回升的变化规律(P<0.05).结果提示,Leu可不依赖于胰岛素直接长期调控山羊胰腺α-淀粉酶的分泌.%The experiment was conducted to investigate the effect of duodenal infusion of leucine (Leu) on pancreatic exocrine secretion and plasma indices in goats. Four one-year-old goats [(30.1±1.3) kg] fitted with duodenal fistulas and common bile duct cannulas were used in a 4 x 4 Latin square design. Each goat re ceived four treatments: infusion of 0, 3, 6 and 9 g/d Leu into duodenum, respectively. The results showed that duodenal infusion of Leu didn' t affect concentrations of glucose and insulin in plasma, total pancreatic juice secretion, total protein concentration and production rate in pancreatic juice, and activities of trypsin, chymotrypsin, lipase and pancreatic α-amylase (P>0.05). However, the secretion rate of pancreatic α-am ylase was increased when the infusion level of Leu was 3 or 9 g/d (P <0.05). The results indicate that Leu can directly long-term regulate the secretion of pancreatic a-amylase independent of insulin in goats.

  6. Desempenho e morfometria da mucosa de duodeno de frangos de corte alimentados com farelo de canola, durante o período inicial Performance and duodenum mucous morphometry of broiler chickens fed canola meal, during the starting period

    Directory of Open Access Journals (Sweden)

    Denise Fontana Figueiredo

    2003-12-01

    and the rations were weighed and at the 21st day two birds of each experimental unit were sacrificed for collection of a duodenum segment for evaluation of intestinal morphometry. The results demonstrated that increasing levels of canola meal induced to a linear reduction in weight gain, weight and feed intake and worst feed conversion. During the growing period (21 to 41 days, in which all of the birds received similar diet, a decrease was observed in the weight and weight gain, with the increase of the levels of canola meal, while parameters feed conversion and feed intake were similar. Data regarding mucous morphology, submitted to the regression analysis, demonstrated that there was increase in crypt depth in accordance of increasing levels of canola meal. It is possible to include up to 20% of canola meal without damages on broiler performance and in the digestive tract.

  7. Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum Efeito crônico da vagotomia sobre a morfometria do plexo mioentérico do duodeno de ratos

    Directory of Open Access Journals (Sweden)

    Carlos Roberto Martins-Júnior

    2010-09-01

    Full Text Available BACKGROUND: The gastrointestinal disorders have been associated with morphological alterations in the myenteric nervous plexus. AIM: To evaluate, through morphometric studies, the chronic effects of the subdiaphragmatic trunk vagotomy on the nervous plexus. METHODS: Fifteen male exemplars of Wistar Rattus novergicus weighing about 150g, distributed into three groups, have been used: control (n=5, Sham (n=5 and vagotomized (n=5. The animals were sacrificed after 30 and 90 days post surgery. Fragments of duodenum were fixed in Bouin solution, embedded into paraffin and stained with HE and PAS. Morphometric analysis was performed by a Carl Zeiss KM 450 image system. The following aspects were observed: the density of nervous cells per linear micrometer (µm (ND; the area of perikarya (µm² (NA; the number of satellite cells per µm (SCD; and the number of satellite cells per neuron (SC/N. The averages were compared with the help of "software" program Sigma Plus through two way - ANOVA and Tuckey post-test. RESULTS: Denervation increased SC/N (pRACIONAL: As disfunções gastrintestinais têm sido associadas à alterações morfológicas no plexo nervoso mioentérico. OBJETIVO: Avaliar através do estudo morfométrico, os efeitos crônicos da vagotomia troncular subdiafragmática sobre esse plexo nervoso. MÉTODOS: Foram utilizados 15 exemplares machos de Rattus novergicus da variedade Wistar, com cerca de 150 g, distribuídos nos grupos controle (n=5, Sham (n=5 e vagotomizados (n=5. Os animais foram sacrificados depois de 30 e 90 dias após as operações. Em seguida, fragmentos do duodeno foram fixados em solução de Bouin, incluídos em parafina e corados por HE e PAS. A análise morfométrica foi realizada por meio do sistema de análise de imagem Carl Zeiss KM 450. Foram observados: a densidade de células nervosas por micrômetro linear (µm; a área dos pericários (µm²; o número de células satélites por µm; e o número de células sat

  8. Trolox reduces the effect of ethanol on acetylcholine-induced contractions and oxidative stress in the isolated rabbit duodenum El Trolox reduce el efecto del etanol sobre las contracciones inducidas a la acetilcolina y el estrés oxidativo en duodeno aislado de conejo

    Directory of Open Access Journals (Sweden)

    Diego S. Fagundes

    2011-08-01

    Full Text Available Trolox is a hydrophilic analogue of vitamin E and a free radical scavenger. Ethanol diminishes the amplitude of spontaneous contractions and acetylcholine (ACh-induced contractions in rabbit duodenum. The aim of this work was to study the effect of Trolox on the alterations induced by ethanol on contractility and lipid peroxidation in the duodenum. The duodenal contractility studies in vitro were carried out in an organ bath and the levels of malondialdehyde and 4-hydroxyalkenals (MDA+4-HAD were measured by spectrophotometry. Trolox increased the reduction induced by ethanol on the amplitude of spontaneous contractions in longitudinal muscle but not in circular muscle. Trolox 4 mM decreased the effects of ethanol on ACh-induced contractions and on MDA+4-HDA concentrations. We conclude that Trolox might prevent oxidative stress induced by ethanol in the duodenum.El Trolox es un análogo hidrofílico de la vitamina E y un agente que secuestra radicales libres. El etanol disminuye la amplitud de las contracciones espontáneas y las contracciones inducidas a la acetilcolina en el duodeno de conejo. El objetivo de este trabajo era estudiar el efecto del Trolox en las alteraciones inducidas por el etanol sobre la contractilidad y la peroxidación lipídica en el duodeno. Los estudios de contractilidad duodenal in vitro se realizaron en un baño de órganos y los niveles de MDA+4-HDA se midieron por espectofotometría. El Trolox aumentó la reducción inducida por el etanol sobre la amplitud de las contracciones espontáneas en el músculo longitudinal pero no en el músculo circular de duodeno. El Trolox 4 mM redujo los efectos del etanol sobre las contracciones inducidas a la acetilcolina y sobre las concentraciones de MDA+4-HDA. Se concluye que el Trolox podría prevenir el estrés oxidativo inducido por el etanol en el duodeno.

  9. Surgical treatment and prognosis of cancers of hepatic flexure of colon invading the duodenum in 65 patients%65例患者结肠肝曲癌侵犯十二指肠的外科治疗及预后

    Institute of Scientific and Technical Information of China (English)

    Weiliang Yang; Shenglong Li; Lei Zhang; Fujing Wang; Yulin Ma

    2011-01-01

    Objective:The aim of the study was to discuss surgical treatment of right colon carcinoma of hepatic flexure invading the duodenum.Methods:The 65 patients with right colon carcinoma of hepatic flexure invading the duodenum,treated in our department from 1987 to 2007,were included in this study.Their clinicopathological data were retrospectively reviewed and analyzed.All the cases were divided into three types (local invasion,regional invasion,and cancer with internal fistula) according to duodenal defect,including local invasion ( 2.0 cm) and the presence of internal fistula.Results:The 25 patients with local invasion underwent en bloc resection of the duodenal wall.Pedicled ileal flap was used to cover the large duodenal defect measuring 2.0–3.0 cm in 5 patients.Dudenojejunostomy was used to reconstruct the large defect measuring more than 5 cm in 3 patients.Conservative resection of right-sided colon was performed in 18 patients with wide invasion.Four patients underwent pancreaticoduodenectomy combined with right hemicolectomy for colon cancer involving the pancreatic head.Ten cases underwent duodenal diverticularization.One patient with anastomotic leak-age healed within 3 weeks.Other patients were cured without postoperative complications.The total 3- and 5-year survival rates after surgery were 53.8% and 9.2%,respectively.Conclusion:The surgical procedure to be performed is usually de-cided according to the cancer location,extent,and duodenal defect and invasion,which are important for prolonging life time,improving of quality of life and prognosis in these patients.

  10. Effects of maternal proteic undernutrition on the neurons of the myenteric plexus of the duodenum of rats Efeitos da desnutrição proteica materna sobre os neurônios do plexo mientérico do duodeno de ratos

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    Maria Raquel Marçal Natali

    1996-06-01

    Full Text Available The purpose of this study was to verify the effects of proteic undernutrition on the neurons of the myenteric plexus from the duodenum of Wistar rats. Twenty-four animals at the age of 60 days were divided in four groups, which were named according to the period their mothers received hypoproteic ration (8%. Some segments of duodenum were subjected to histological treatment and stained with hematoxilin-eosin and some were used for whoie mount preparations stained with Giemsa. We observed small, medium-sized and large neurons grouped in ganglia of various shapes. It was concluded that the maternal proteic undernutrition does not affect the organization of the myenteric plexus and that animals submitted to undernutrition during gestation and lactation, when normally fed, show neurons with strongly basophilic cytoplasm and larger cellular bodies than those from control animals.O propósito deste estudo foi investigar os efeitos da desnutrição proteica sobre os neurônios do plexo mientérico do duodeno de ratos Wistar. Vinte e quatro animais com 60 dias de idade foram divididos em 4 grupos e denominados de acordo com o período em que suas mães receberam ração hipoproteica (8%. Alguns segmentos de duodeno foram submetidos a tratamento histológico e corados com hematoxilina-eosinae alguns foram utilizados na elaboração dos preparados de membrana corados com Giemsa. Observamos neurônios pequenos, médios e grandes formando gânglios de formatos diversos. Concluiu-se que a desnutrição proteica materna não afeta a organização do plexo mientérico e que animais desnutridos na gestação e lactação quando voltam a receber dieta com teor proteico normal apresentam neurônios com citoplasma fortemente basofílico e geralmente maiores que o de animais normo-alimentados.

  11. Symptomatic Primary (AL Amyloidosis of the Stomach and Duodenum

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    Reidar Fossmark

    2013-01-01

    Full Text Available Primary (AL amyloidosis of the gastrointestinal tract is relatively rare, and symptomatic amyloidosis of the stomach is even more seldom. We present the case of a patient who was referred to upper endoscopy because of weight loss, nausea, and vomiting. Large areas of intramucosal hemorrhages were seen, and biopsies resulted in profuse bleeding stopped with endoscopic clips. The biopsies showed amyloid depositions and further workup revealed that the patient also had cardiac and neuropathic involvements. The patient started treatment with dexamethasone, melphalan and bortezomib. After treatment was started the nausea and epigastric discomfort improved, and a reduction in the biochemical markers troponin T, NT-proBNP, and M-component was observed. Gastric amyloidosis is rarely seen at upper endoscopy in patients without a previously established diagnosis, but the unusual endoscopic findings and bleeding tendency after biopsy should be kept in mind by gastroenterologists.

  12. A large gastrointestinal stromal tumor of duodenum: a case report

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    Hadi Ahmadi Amoli

    2014-01-01

    Case presentation: The patient is a forty six years old man. He complained of frequent colic pain in left upper quadrant of abdomen for two months before admitting to the hospital. The pain resolved spontaneously after a few hours. This situation almost has been repeated every week. The patient had severe repeated melena and faint for two weeks. As soon as the patient was entered the Sina Hospital in 2012, supportive care was started. Then upper gastrointestinal endoscopy was performed for him and the bleeding point was detected. Also abdominal and pelvic computed tomography with oral and intravenous contrast was done. Finally the patient was operated on tumor diagnosis in duodenal area according to classic Whipple procedure. Conclusion: Gastrointestinal bleeding is the most common symptom of gastrointestinal stromal tumors. The bleeding is minimal and chronic. It will be progress to sudden and severe bleeding. Diagnosis is done by upper gastrointestinal endoscopy and biopsy. The large tumors with high mitotic

  13. Programmed cell death and cell extrusion in rat duodenum

    DEFF Research Database (Denmark)

    Schauser, Kirsten; Larsson, Lars-Inge

    2005-01-01

    The small intestinal epithelium is continously renewed through a balance between cell division and cell loss. How this balance is achieved is uncertain. Thus, it is unknown to what extent programmed cell death (PCD) contributes to intestinal epithelial cell loss. We have used a battery...... of techniques detecting the events associated with PCD in order to better understand its role in the turnover of the intestinal epithelium, including modified double- and triple-staining techniques for simultaneously detecting multiple markers of PCD in individual cells. Only a partial correlation between TUNEL...... positivity for DNA fragmentation, c-jun phosphorylation on serine-63, positivity for activated caspase-3 and apoptotic morphology was observed. Our results show that DNA fragmentation does not invariable correlate to activation of caspase-3. Moreover, many cells were found to activate caspase-3 early...

  14. Gallstone ileus of duodenum with huge duodenal stone

    OpenAIRE

    Mohammad Ehsan; Mohammad Reza Ahangaran

    2006-01-01

    Gastrointestinal obstruction by a gallstone is an uncommon but important complication of biliary stone disease which mostly affects the elderly. The classic triad of radiological features includes pneumobilia, ectopic gallstone and evidence of intestinal obstruction. Terminal ileum is the most common site of obstruction, followed by jejunum and gastric outlet. We present a case of duodenal gallstone ileus of a large, fluid-density mixed biliary stone with a peripheral rim of hyperdensity (ver...

  15. Crohn's disease confined to the duodenum: A case report.

    Science.gov (United States)

    Song, Dong Jin; Whang, Il Soon; Choi, Hyung Wook; Jeong, Cheol Yun; Jung, Sung Hoon

    2016-06-16

    Crohn's disease (CD) can involve any part of the gastrointestinal tract from the mouth to anus. However, gastroduodenal CD is rare with a frequency reported to range between 0.5% and 4.0%. Most patients with gastroduodenal CD have concomitant lesions in the terminal ileum or colon, but isolated gastroduodenal Crohn's disease is an extremely rare presentation of the disease accounting for less than 0.07% of all patients with CD. The symptoms of gastroduodenal CD include epigastric pain, dyspepsia, early satiety, anorexia, nausea, vomiting, and weight loss. The diagnosis of gastroduodenal CD requires a high level of clinical suspicion and can be made by comprehensive clinical evaluation. Here we report a rare case of isolated duodenal CD not confirmed by identification of granuloma on biopsy, but diagnosed by clinical evaluation.

  16. Tachykinergic neurotransmission is enhanced in duodenum from dystrophic (mdx) mice

    OpenAIRE

    Zizzo, Maria Grazia; Mulè, Flavia; Serio, Rosa

    2005-01-01

    Duodenal longitudinal muscle of mdx mice, an animal model for Duchenne muscular dystrophy, showed a decrease in the electrically evoked nonadrenergic, noncholinergic (NANC) inhibitory responses associated with a reduction of the participation of nitric oxide (NO). In this study, we investigated whether the impairment of NO could also lead to alterations in the NANC excitatory transmission.Nerve-evoked responses consisted of an inhibitory phase followed, at the end of stimulation, by an excita...

  17. Testicular seminoma metastasis to duodenum. Misdiagnosed as primary duodenal tumor

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    Amer Hashim Al Ani

    2016-01-01

    Conclusion: High index of suspicion for testicular seminoma must be raised when treating young males with GIT complications like hemorrhage. Testicular seminoma is the most common solid tumor at this age. Sometimes it is the cause behind this complication.

  18. Role of duodenum in regulation of plasma ghrelin level and body mass index after subtotal gastrectomy%胃次全切除术后十二指肠调节血浆ghrelin水平及人体质量指数的作用

    Institute of Scientific and Technical Information of China (English)

    王海涛; 卢麒丞; 王勃; 王荣朝; 张云; 陈海龙; 钱海鑫

    2008-01-01

    目的 研究胃次全切除术后十二指肠调节血浆ghrelin水平与人体质量指数(BMI)的作用.探讨ghrelin与BMI之间的关系.方法 选择42例行胃癌根治手术的T1-2N0-1M0胃癌患者,根据消化道重建方式分为毕Ⅰ式组(23例)和毕Ⅱ式组(19例).分别测定患者术前、术后第1、7、30和360天的血浆ghrelin水平及BMI.结果 两组患者术后早期ghrlein水平下降幅度基本一致:第1天下降至最低(36.7%比35.7%).第7天均有所回升(51.0%比51.1%),两组差异无统计学意义(P>0.05).第30天,两组ghrelin水平分别恢复至70.6%和67.2%,毕Ⅰ式组较毕Ⅱ式组有增高趋势.第360天时,毕Ⅰ式组ghrelin水平恢复至93.1%,虽低于术前水平、但差异无统计学意义(P>0.05);而毕Ⅱ式组仅恢复至81.6%,明显低于术前水平(P=0.033);两组ghrelin水平差异有统计学意义(P=0.035).与术前相比,两组患者ghrelin水平在术后360天分别下降6.9%和18.4%;BMI下降3.3%和6.4%.ghrelin和BMI下降幅度之间两组均存在着线性关系(R12=0.297,P=0.007;R22=0.559,P<0.01).结论 符合解剖生理的十二指肠能代偿性地促进术后患者血浆ghrelin水平的恢复,相应地促进BMI的恢复.ghrelin分泌不足的患者ghrelin水平与BMI呈正相关.%Objective To investigate the role of duodenum in regulation of ghrelin and body mass index (BMI) and the correlation between ghrelin and BMI after subtotal gastrectomy.Methods Forty-two patients with T0-1N0-1M0 gastric cancer were divided into two groups after gastrectomy according to digestive reconstruction pattern,Bi]lroth Ⅰ group (n=23) and Billroth Ⅱ group (n=19) respectively.Plasma ghrelin levels were determined by radioimmunoassay (RIA) before and at day 1, 7, 30 and 360 after gastreetomy,and BMIs were also measured. Results Two groups had identical postoperative trends in ghrelin alterations during the early stage, both dropping to nadir at day 1 (36.7% vs 35.7%), then markedly increasing at day 7

  19. Estudo morfoquantitativo do plexo mioentérico do duodeno de ratos submetidos a carência de proteínas e vitaminas do complexo B - DOI: 10.4025/actascibiolsci.v26i2.1642 Morphoquantitative study of the duodenum myenteric plexus from rats subjected to lack of proteic and complex B vitamins - DOI: 10.4025/actascibiolsci.v26i2.1642

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    Sonia Lucy Molinari

    2004-04-01

    Full Text Available Este estudo teve como objetivo avaliar os efeitos das carências protéica e vitamínica sobre os neurônios do plexo mioentérico do duodeno de ratos. 24 animais, aos 90 dias de idade, foram divididos em dois grupos: controle (n=12 e experimental (n=12. Durante 120 dias, o grupo controle recebeu ração com teor protéico de 22%, vitaminas e minerais; o grupo experimental, 8%, sem suplementação de vitaminas. Após período experimental, coletou-se o sangue dos animais e retirou-se o duodeno para análise ultraestrutural e morfoquantitativa (NADH-d; NADPH-d. O grupo experimental apresentou proteínas totais e albumina plasmática reduzidas e peso inferior aos do grupo controle. A densidade (NADPH-d foi maior e significante para o grupo experimental; na técnica da NADH-d, o perfil neuronal diferiu entre os grupos. Os aspectos ultraestruturais mostraram-se semelhantes entre os grupos. Os resultados permitem concluir que a dieta imposta promoveu um quadro moderado de desnutrição, não provocando alterações quantitativas, porém levou a alterações no perfil neuronalThis study aims to evaluate the effects of protein and vitamin deficiency on the neurons of the duodenum myenteric plexus of rats. 24 90-day old animals were divided in two groups: control (n=12 and experimental (n=12. During 120 days, the control group received chow with 22% protein level, vitamins and minerals; the experimental group was fed with 8%-protein chow, without vitamin supplementation. After the experimental period, the animals’ blood was collected and the duodenum was removed for ultrastructural and morphoquantitative analyses (NADH-d; NADPH-d. The experimental group had reduced total protein, plasma albumin and body weight lower than the control group. The density (NADPH-d was higher and significant for the experimental group; with the NADH-d technique the neuronal profile differed between the groups. The ultrastructural aspects were similar in both groups. The

  20. 保留十二指肠胰头切除术治疗胰头部肿块型慢性胰腺炎的疗效%Efficacy of duodenum-preserving pancreatic head resection for chronic pancreatitis with mass in the head of the pancreas

    Institute of Scientific and Technical Information of China (English)

    孙诚谊; 朱海涛

    2014-01-01

    Objective To investigate the clinical efficacy of two types of duodenum-preserving pancreatic head resection (Beger procedure and Berne procedure) for chronic pancreatitis with mass in the head of the pancreas.Methods The clinical data of 46 patients with chronic pancreatitis and mass in the head of the pancreas who were admitted to the Affiliated Hospital of Guiyang Medical College from September 2008 to April 2012 were retrospectively analyzed.There were 24 patients received Beger procedure (Beger group),and 22 received Beme procedure (Berne group).The complications,life quality and pain after the operation were evaluated.Patients were followed up via phone call and out-patient examination till April 2013.The measurement data were analyzed using the Mann-Whitney U test,and the constituent ratios were compared using the chi-square test.Results The operation time and volume of blood loss were (377 ± 21) minutes and (746 ± 129) mL in the Beger group,and (323 ± 17) minutes and (577 ± 111)mL in the Berne group,with significant difference between the 2 groups (U=14.0,88.0,P <0.05).Four patients in the Beger group and 1 in the Berne group were complicated with pancreatic leakage,with no significant difference between the 2 groups (x2=0.714,P > 0.05).The scores of life quality evaluation (physical condition,work capacity,cognitive ability,emotion,social competence and overall life quality) were 82 ± 14,74±24,90 ± 18,78±20,83 ± 18,73 ± 18 in the Beger group,and 79 ± 16,71 ±20,92 ±21,76 ± 18,80 ±21,70 ± 16 in the Berne group,with no significant difference between the 2 groups (U =177.5,183.5,187.5,178.0,189.5,192.0,P > 0.05).The scores of symptom evaluation (fatigue,nausea and vomitting,pain,anorexia,dyspnea,sleep disorders,obstipation,diarrhea,financial worries) were 28 ± 16,24 ± 10,20±12,23 ± 14,4 ± 1,32 ± 12,6 ±2,18 ± 14,36± 18 in the Beger group,and 26 ± 18,26 ±20,22 ± 16,26 ± 16,3 ± 1,30 ± 10,5 ± 1,16 ± 12,38 ± 20 in the Berne

  1. Description of the technique of upper gastrointestinal series radiological examination for the evaluation of the esophagus, stomach and duodenum of Wistar female rats Descrição de técnica radiológica contrastada de esôfago, estômago e duodeno em ratas Wistar

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    João Vicente Machado Grossi

    2009-12-01

    Full Text Available PURPOSE: To evaluate the anatomy of female Wistar rats and the workability of contrast radiography as a technique to investigate the gastrointestinal series. METHODS: Eight adult female Wistar rats were undergone to the contrast radiography as anteroposterior incidence and as posterior incidence in profile. The radiological examination was conducted at a 45° angle to the radiological table. Film-focus distance (FFD was 100 cm, film-object distance (FOD was 0 cm, and object-focus distance (OFD was 100 cm. An orogastric probe was used to inject barium contrast at 5-min intervals, for a total of four applications. After the radiological examination, animals were necropsy for confirmation of the radiological findings, and the radiographs were the absence of the normal anatomy variations inspected and described by an experienced radiologist. RESULTS: All the radiographs produced achieved satisfactory results in terms of position, exposure, location and quality. The upper esophageal sphincter (UES was identified in the esophagus at the nerve C2, the lower esophageal sphincter (LES was identified between spinal cord segments L1 and L2, the thoracic-abdominal junction was observed at T10, the esophageal-gastric junction (EGJ at T13-L1, with the abdominal portion in the epigastric region. The stomach was observed mostly in the epigastric region, left hypochondrium, left and mesogastric flank. The duodenum findings presented higher variation, with most findings identified in the epigastric region, right hypochondrium, right flank and mesogastric ileal fossa at T13-L5. CONCLUSION: Contrast radiology is useful and may be employed to assess the anatomy of the animal being studied. The experimental model described afforded to fully identify all organs investigated, as well as other occasional relevant findings. No anatomical anomalies in the subsequent necropsy, confirming the radiographic findings.OBJETIVO: Avaliar a anatomia normal de ratas Wistar e a

  2. 保留十二指肠的胰头切除与保留幽门的胰十二指肠切除治疗胰头肿块型胰腺炎的Meta分析%A meta-analysis on surgical treatments for chronic pancreatitis: duodenum-preserving pancreatic head resection versus pylorus-preserving pancreaticoduodenectomy

    Institute of Scientific and Technical Information of China (English)

    汪超; 黄强; 林先盛; 刘臣海; 杨骥

    2015-01-01

    Objective To compare the safety and effectiveness of duodenum-preserving pancreatic head resection (DPPHR) with pylorus-preserving pancreaticoduodenectomy (PPPD) in the treatment of chronic pancreatitis with a pancreatic head mass.Methods Medline,Biosis,Cochrane Library,Science Citation Index Database,CBM Database,Wan Fang and CNKI were searched systematically.The bias risk of the included trials was assessed according to the assessing tools as suggested by the Cochrane Handbook.Review Manage 5.2 was used to perform the statistical analysis.Results 7 RCTs with 226 patients were included in the meta-analysis which showed that there were no significant differences between PPPD and DPPHR in overall postoperative morbidity,postoperative hospital stay,complete pain relief,pancreatic fistula,exocrine insufficiency,symptom score at 5 to 7-year follow-up,and quality of life score at 14 to 15-year follow-up (P > 0.05).While DPPHR had significant superiorities in operation time,blood replacement,delayed gastric emptying,occupational rehabilitation after the operations,weight gain,quality of life score at 1 to 2-year follow-up,symptom score at 5 to 7-year follow-up,and physical functioning score at 14 to 15-year follow-up.Conclusions DPPHR is more favourable than PPPD in reducing the use of blood replacement,shortening operation time,delayed gastric emptying,occupational rehabilitation after the operations,weight gain,physical functioning,and in improving quality of life of patients.%目的 探讨保留十二指肠的胰头切除术(DPPHR)与保留幽门的胰十二指肠切除术(PPPD)治疗胰头肿块型胰腺炎的安全性和有效性.方法 系统检索Medline、Biosis、The Cochrane Library、Science Citation Index Database、中国生物医学文献数据库、中国期刊全文数据库、万方数据库、维普中文科技期刊数据库.选取相关临床随机对照试验,参考Cochrane Handbook的偏倚风险评估工具评估偏倚风险.应用Review Manager

  3. Iron Malabsorption in a Patient With Large Cell Lymphoma Involving the Duodenum

    Science.gov (United States)

    1992-01-01

    111-37. coeliac disease . Lancet 1960:1:192-4. 7. Shreeve DR. Horrocks P. Mainwaring AR. Steatorrhea and intra- 20. Green PA. Wollaeger EE. The clinical...compounded the anemia in a pa- tion in celiac disease were reversible by the institution tient with diffuse large cell lymphoma involving the of a gluten...hemoglobin. The lymphomas (5-7). The presenting symptoms mimic chest radiograph in May demonstrated an anterior me- those of celiac disease and include

  4. Uncomplicated spontaneous rupture of the pancreatic pseudocyst into the duodenum: Case report

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    Ali Emre Atıcı

    2014-06-01

    Full Text Available Pancreatic pseudocyst is one of the common complications of acute and chronic pancreatitis. Although most pseudocysts resolve spontaneously with supportive care, larger pseudocysts are more likely to cause complications. Large pancreatic pseudocysts may rupture spontaneously into the gastrointestinal tract and are usually associated with life-threatening bleeding. Surgical treatment or interventional drainage procedures may be necessary. Uncomplicated rupture of pseudocyst is extremely rare.

  5. Viability of microflora pf market fermented milk products in simulated conditions of gastric and duodenum

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    Małgorzata Ziarno

    2010-06-01

    Full Text Available Background. The non-probiotic lactic acid bacteria have only rarely been used in in vitro or in vivo studies, because they are not considered to exert health benefits. To exert the beneficial effect in human organism, LAB needs to meet some criteria, for example the viability in the gastrointestinal tract (GIT. The aim of this work was to determine the viability of microflora of chosen market non-probiotic fermented milk products in simulated gastric and duodenal fluids. Material and methods. Ten market non-probiotic fermented milk products bought in Warsaw were used in this study. Ten grams of each product have been suspended in simulated gastric fluid, and then the mixture has been transferred into simulated duodenal fluid. Immediately after bacteria inoculums addition and at the end of the experiment, the number of lactobacilli and lactococci was measured. Results. The number of lactococci or streptococci decreased by 0.1-0.3 log cycle after 3 h in gastric mixture. Only in one yoghurt the population of streptococci decreased by 0.9 log cycle. The population of lactobacilli did not change in condition of simulated gastric fluid. The significant reduction of lactobacilli, lactococci and streptococci population was observed after the transfer of mixture into simulated duodenal fluid and incubation in this condition for 5 h. After the end of experiments in every studied sample the number of microflora remained at the level above 6 log CFU/mL. Conclusions. The results indicate that the condition simulating gastric fluid is not a menace to viability of lactic acid bacteria, if they are protected by milk products. The significant reduction of bacteria number in simulated duodenal fluid was probably caused by cell shock to intensive pH change of environment and loss of protective barrier caused by digestive enzymes activity.

  6. Pancreatic Arteriovenous Malformation Involving the Duodenum Embolized with Ethylene-Vinyl Alcohol Copolymer (Onyx)

    Energy Technology Data Exchange (ETDEWEB)

    Grasso, Rosario Francesco, E-mail: r.grasso@unicampus.it; Cazzato, Roberto Luigi; Luppi, Giacomo; Faiella, Eliodoro; Del Vescovo, Riccardo; Giurazza, Francesco [University ' Campus Bio-Medico of Rome' , Department of Radiology (Italy); Borzomati, Domenico; Coppola, Roberto [University ' Campus Bio-Medico of Rome' , Department of General Surgery (Italy); Beomonte Zobel, Bruno [University ' Campus Bio-Medico of Rome' , Department of Radiology (Italy)

    2012-08-15

    Arteriovenous malformation (AVM) of the pancreas is a rare condition. Most patients are asymptomatic or alternatively may present with a wide spectrum of symptoms. Traditionally, surgery has been considered the treatment of choice; however, alternative approaches, such as transcatheter embolization (TAE), may be proposed. We report a case of a 48-year-old man with a pancreatic head AVM, presenting with upper abdominal pain and slight anemia. The patient refused surgery and underwent TAE by means of ethylene-vinyl alcohol copolymer (EVOH). At 3 months follow-up, the patient was able to eat regularly, with no residual pain and no signs of anemia.

  7. Inflammatory fibroid polyp in the duodenum of a common marmoset (Callithrix jacchus).

    Science.gov (United States)

    Yokouchi, Yusuke; Imaoka, Masako; Sayama, Ayako; Jindo, Toshimasa; Sanbuissho, Atsushi

    2013-01-01

    A 32-month-old male common marmoset had a firm and white-colored mass in the duodenal wall. The cut surface was smooth and grayish white in color. Histologically, the mass consisted of a proliferation of spindle cells with an oval to spindle-shaped nucleus and scant eosinophilic cytoplasm in a loose myxoid or fibrotic background. Most of the lesion displayed no specific growth pattern whereas some of the cells concentrated around the vessels and created an onion-bulb structure. Additionally, marked inflammatory cellular infiltration, mainly eosinophils, was observed throughout the lesion. Immunohistochemically, the spindle cells were positive for vimentin, α-smooth muscle actin, fascin, and cyclin D1, and negative for S-100, factor VIII-related antigen, and c-kit. These histological and immunohistochemical features did not meet any differential diagnoses such as gastrointestinal stromal tumor, inflammatory myofibroblastic tumor, solitary fibrous tumor/hemangiopericytoma, smooth muscle tumor, schwannoma, and hemangiosarcoma. Collectively, the authors diagnosed the mass as a lesion that corresponded to an inflammatory fibroid polyp (IFP) in humans. IFP is defined as a mesenchymal proliferation composed of spindle stromal cells, small blood vessels, and inflammatory cells, particularly eosinophils, and is currently classified as a nonneoplastic lesion. To the best of our knowledge, this is the first case of spontaneous IFP in nonhuman primates.

  8. Carcinoid tumor of the duodenum and accessory papilla associated with polycythemia vera

    Institute of Scientific and Technical Information of China (English)

    Horng-Yuan Wang; Ming-Jen Chen; Tsen-Long Yang; Ming-Chih Chang; Yu-Jan Chan

    2005-01-01

    Carcinoid tumors have been reported in a wide range of organs but most frequently involve the gastrointestinal tract; however, duodenal carcinoid tumors are rare. We report a 50-year-old male patient complaining of multiple melenas for 3 wk. The panendoscopy and endoscopic retrograde cholangiopancreaticography revealed swelling accessory papilla with an ulcer. The biopsy taken showed a carcinoid tumor. The lesion was removed by wide resection. Patient was found to have an abnormal blood cell count during the follow-up period with elevated levels of hemoglobin and hematocrit of 21.2 g/dL and 63.5%,respectively, thrombocytosis of 501 000/μL, and leukocytosis of 20 410/μL. He was diagnosed as a polycythemia vera by a hematologist after further evaluation. He received periodic phlebotomy and hydroxyurea treatment. The response was good and his hematocrit was stabilized by periodic phlebotomy in the range of 44-49% during the last 2 years. The possible origin of UGI bleeding by a duodenal carcinoid tumor, although rare, should be considered. There has been one case report of a duodenal carcinoid tumor that involved accessory papilla of the pancreas divisum and one case report of metastatic carcinoid tumor associated with polycythemia vera. It is different in our patient as compared with the latter report, which mentioned a polycythemia vera patient who was found to have a metastatic carcinoid in the 17 years follow-up period. Chemotherapy had been given before the carcinoid tumor was revealed. Our patient had no previous chemotherapy for polycythemia vera before he was found to have duodenal carcinoid tumor; this excludes the possibility of chemotherapy induced carcinoid tumor, although it had been suspected in the previous report. In our patient, the existence of both diseases may be by predisposition of each other since both diseases have an increased incidence of other neoplasm, or they may be coexistent incidentally.

  9. Expression of nutrient transporters in duodenum, jejunum and ileum of Eimeria maxima- infected broiler chickens

    Science.gov (United States)

    The intracellular parasite, Eimeria maxima, invades epithelial cells of the intestine causing malabsorption, diarrhea, and decreased feed conversion resulting in significant economic losses to poultry producers. The uptake of amino acids is mediated by active transporters located on the basal and br...

  10. Gallbladder ascariasis with uneventful worm migration back to the duodenum: A case report.

    Science.gov (United States)

    Mushtaque, Majid; Khan, Parwez Sajad; Mir, Mohammad Farooq; Khanday, Samina Ali

    2012-04-01

    Ascariasis, a worldwide parasitic disease, is regarded by some authorities as the most common parasitic infection in humans. The causative organism is Ascaris lumbricoides, which normally lives in the lumen of the small intestine. From the intestine, the worm can invade the bile duct or pancreatic duct, but invasion into the gallbladder is quite rare because of the anatomical features of the cystic duct, which is narrow and tortuous. Once it enters the gallbladder, it is exceedingly rare for the worm to migrate back to the intestine. We report a case of gallbladder ascariasis with worm migration back into the intestine, in view of its rare presentation.

  11. [Features of the structure of the regional lymph nodes of the duodenum in monkeys].

    Science.gov (United States)

    Rusina, A K

    1980-05-01

    By means of morphometric methods, duodenal regional lymph nodes were studied in rhesus and lapunder macaques. It was demonstrated that in monkeys the connective tissue framework, cortical plateau, medullary substance, cortical substance, sinuses and follicles are expressed differently. Cellular elements in the lymph nodes analysed in the monkeys subjected to a comparative investigation demonstrated their uneven distribution in the same structural components. Small lymphocytes were predominate cellular elements. There were rather essential differences in the number of plasmic cells, mitotically dividing cells, acidophilic granulocytes, mast cells and macrophages. Certain species differences were demonstrated to exist both in structure and cell composition of the lymph nodes that seemed to depend on some local peculiarities of immunogenic reactions.

  12. Effect of abomasal butyrate infusion on gene expression in the duodenum of lambs

    Science.gov (United States)

    A previous study infusing butyrate into the abomasum of sheep produced increased oxygen, glucose, glutamate, and glutamine uptake by the portal-drained viscera. These changes were thought to be partially due to increases in glycolysis and cell proliferation. The purpose of this study was to evaluate...

  13. Consecutive en-bloc liver (30%)-pancreas-duodenum-spleen-stomach transplant in Lewis rats.

    Science.gov (United States)

    Yoo, C H; Hong, I C; Lee, S; Nam, S; Bai, S; Kim, K; Pivetti, C D; Niewiadomski, S T; Wolf, P; Gittes, R F

    2003-01-01

    It is well-known that 30% of the remaining liver mass, following partial hepatectomy, regenerates to full original mass within 2 weeks in rats. In order to carry the transplanted rat liver to repeated transplantation, a technique of combining 30% of the liver with the pancreaticoduodenum and spleen transplantation is performed in this consecutive organ transplantation study. Our laboratory observed several 37-month-old transplanted rats by carrying through 2-3 generations, and histological disclosure were made. Because the partial liver transplants did not regenerate after the transplantation with other splanchnic organs, this technique is not so difficult though subsequent surgical maneuvers are needed and the liver histology proved entirely normal in every aspect when followed beyond the rat's life span of 24 months.

  14. Neuropeptide Y (NPY): immunohistochemical determination in duodenum of intermediate stage of horses fetuses

    OpenAIRE

    Dauria, Pascual; Mac Loughlin, Virginia; Rinaldi, V.; Sagripanti, Graciela; Giménez, Sabrina; Sona, Liliana; Bonino, Facundo; Navarro, Osvaldo; Martínez, Ramiro

    2015-01-01

    Las hormonas regulan diferentes funciones del organismo entre las que se incluyen aquellas vinculadas con el aparato digestivo, lo que resulta interesante por la asociación de éste con distintas etapas del desarrollo del individuo. Para poder aportar nuevos conocimientos, muchos son los aspectos a considerar como por ejemplo el estudio de las sustancias químicas involucradas en el proceso alimentario, como las hormonas gastrointestinales. El objetivo es determinar la presencia de Neuropéptido...

  15. HCO3- secretion in rat duodenum after treatment with omeprazole and ranitidine.

    Science.gov (United States)

    Knutson, L; Flemström, G; Gustavsson, S; Jedstedt, G; Lönnerholm, G

    1987-01-01

    The bicarbonate secretion by the duodenal mucosa, which is stimulated by luminal acid, is very probably important in mucosal protection against the acid. It was of interest to investigate whether long-term deprivation of the mucosa of this acid stimulus affected the alkali secretion. Sprague-Dawley rats were treated for 4-6 weeks with either omeprazole, 14 mg/kg body weight twice daily, or ranitidine, 300 mg/kg twice daily, by means of gastric intubation. The rate of bicarbonate secretion by the duodenal mucosa was determined in situ by continuous titration. Neither the basal secretion nor the increase in secretion in response to stimulation by prostaglandin E2 or luminal acid (pH 2.0 for 5 or 60 min) differed in treated animals from that in controls that had received placebo (p greater than 0.05). Thus, 4-6 weeks of treatment with omeprazole or ranitidine did not reduce duodenal mucosal bicarbonate secretion in the rat, nor did these drugs diminish the ability of this mucosa to respond to prolonged luminal acidification or luminally administered prostaglandin E2.

  16. Activating GNAS and KRAS mutations in gastric foveolar metaplasia, gastric heterotopia, and adenocarcinoma of the duodenum

    OpenAIRE

    Matsubara, A.; Ogawa, R; Suzuki, H; Oda, I.; Taniguchi, H; Kanai, Y.; Kushima, R; Sekine, S

    2015-01-01

    Background: Heterotopic gastric-type epithelium, including gastric foveolar metaplasia (GFM) and gastric heterotopia (GH), is a common finding in duodenal biopsy specimens; however, there is still controversy regarding their histogenetic backgrounds. Methods: We analysed a total of 177 duodenal lesions, including 66 GFM lesions, 81 GH lesions, and 30 adenocarcinomas, for the presence of GNAS, KRAS, and BRAF mutations. Results: Activating GNAS mutations were identified in 27 GFM lesions (41%) ...

  17. Mucosal surface morphology and histological changes in the duodenum of the rat following administration of cysteamine

    DEFF Research Database (Denmark)

    Poulsen, Steen Seier; Szabo, S

    1977-01-01

    . The process started in the epithelium of the apical parts of the villi where segments of surface epithelial cells exfoliated and the lamina propria seemed to retract. As the process continued the villi gradually became lower eventually forming an avillous surface. Subsequently defects in the epithelium...

  18. Severe upper gastrointestinal bleeding in extraluminal diverticula in the third part of the duodenum

    OpenAIRE

    2014-01-01

    The successful management of upper gastrointestinal (GI) bleeding requires identification of the source of bleeding and when this is achieved the bleeding can often be treated endoscopically. However, the identification of the bleeding can be challenging due to the location of the bleeding or technical aspects. Therefore it might be necessary to use other measures than endoscopy such as CT angiography. Duodenal diverticula is a rare cause of upper GI bleeding and can be challenging to diagnos...

  19. Severe upper gastrointestinal bleeding in extraluminal diverticula in the third part of the duodenum.

    Science.gov (United States)

    Wilhelmsen, Michael; Andersen, Johnny Fredsbo; Lauritsen, Morten Laksafoss

    2014-05-13

    The successful management of upper gastrointestinal (GI) bleeding requires identification of the source of bleeding and when this is achieved the bleeding can often be treated endoscopically. However, the identification of the bleeding can be challenging due to the location of the bleeding or technical aspects. Therefore it might be necessary to use other measures than endoscopy such as CT angiography. Duodenal diverticula is a rare cause of upper GI bleeding and can be challenging to diagnose as they often require specialised endoscopy procedures such as endoscopy with a side-viewing scope. This case describes the first successful management of this rare condition with an upper GI endoscopy with a colonoscope and afterwards intravascular coiling.

  20. Dipeptidyl peptidase IV inhibition potentiates amino acid- and bile acid-induced bicarbonate secretion in rat duodenum.

    Science.gov (United States)

    Inoue, Takuya; Wang, Joon-Ho; Higashiyama, Masaaki; Rudenkyy, Sergiy; Higuchi, Kazuhide; Guth, Paul H; Engel, Eli; Kaunitz, Jonathan D; Akiba, Yasutada

    2012-10-01

    Intestinal endocrine cells release gut hormones, including glucagon-like peptides (GLPs), in response to luminal nutrients. Luminal L-glutamate (L-Glu) and 5'-inosine monophosphate (IMP) synergistically increases duodenal HCO3- secretion via GLP-2 release. Since L cells express the bile acid receptor TGR5 and dipeptidyl peptidase (DPP) IV rapidly degrades GLPs, we hypothesized that luminal amino acids or bile acids stimulate duodenal HCO3- secretion via GLP-2 release, which is enhanced by DPPIV inhibition. We measured HCO3- secretion with pH and CO2 electrodes using a perfused rat duodenal loop under isoflurane anesthesia. L-Glu (10 mM) and IMP (0.1 mM) were luminally coperfused with or without luminal perfusion (0.1 mM) or intravenous (iv) injection (3 μmol/kg) of the DPPIV inhibitor NVP728. The loop was also perfused with a selective TGR5 agonist betulinic acid (BTA, 10 μM) or the non-bile acid type TGR5 agonist 3-(2-chlorophenyl)-N-(4-chlorophenyl)-N,5-dimethylisoxazole-4-carboxamide (CCDC; 10 μM). DPPIV activity visualized by use of the fluorogenic substrate was present on the duodenal brush border and submucosal layer, both abolished by the incubation with NVP728 (0.1 mM). An iv injection of NVP728 enhanced L-Glu/IMP-induced HCO3- secretion, whereas luminal perfusion of NVP728 had no effect. BTA or CCDC had little effect on HCO3- secretion, whereas NVP728 iv markedly enhanced BTA- or CCDC-induced HCO3- secretion, the effects inhibited by a GLP-2 receptor antagonist. Coperfusion of the TGR5 agonist enhanced L-Glu/IMP-induced HCO3- secretion with the enhanced GLP-2 release, suggesting that TGR5 activation amplifies nutrient sensing signals. DPPIV inhibition potentiated luminal L-Glu/IMP-induced and TGR5 agonist-induced HCO3- secretion via a GLP-2 pathway, suggesting that the modulation of the local concentration of the endogenous secretagogue GLP-2 by luminal compounds and DPPIV inhibition helps regulate protective duodenal HCO3- secretion.

  1. Digestion of phospholipids after secretion of bile into the duodenum changes the phase behavior of bile components.

    Science.gov (United States)

    Birru, Woldeamanuel A; Warren, Dallas B; Ibrahim, Ahmed; Williams, Hywel D; Benameur, Hassan; Porter, Christopher J H; Chalmers, David K; Pouton, Colin W

    2014-08-04

    Bile components play a significant role in the absorption of dietary fat, by solubilizing the products of fat digestion. The absorption of poorly water-soluble drugs from the gastrointestinal tract is often enhanced by interaction with the pathways of fat digestion and absorption. These processes can enhance drug absorption. Thus, the phase behavior of bile components and digested lipids is of great interest to pharmaceutical scientists who seek to optimize drug solubilization in the gut lumen. This can be achieved by dosing drugs after food or preferably by formulating the drug in a lipid-based delivery system. Phase diagrams of bile salts, lecithin, and water have been available for many years, but here we investigate the association structures that occur in dilute aqueous solution, in concentrations that are present in the gut lumen. More importantly, we have compared these structures with those that would be expected to be present in the intestine soon after secretion of bile. Phosphatidylcholines are rapidly hydrolyzed by pancreatic enzymes to yield equimolar mixtures of their monoacyl equivalents and fatty acids. We constructed phase diagrams that model the association structures formed by the products of digestion of biliary phospholipids. The micelle-vesicle phase boundary was clearly identifiable by dynamic light scattering and nephelometry. These data indicate that a significantly higher molar ratio of lipid to bile salt is required to cause a transition to lamellar phase (i.e., liposomes in dilute solution). Mixed micelles of digested bile have a higher capacity for solubilization of lipids and fat digestion products and can be expected to have a different capacity to solubilize lipophilic drugs. We suggest that mixtures of lysolecithin, fatty acid, and bile salts are a better model of molecular associations in the gut lumen, and such mixtures could be used to better understand the interaction of drugs with the fat digestion and absorption pathway.

  2. Pancreas-preserving total duodenectomy versus standard pancreatoduodenectomy for patients with familial adenomatous polyposis and polyps in the duodenum.

    NARCIS (Netherlands)

    Castro, SM de; Eijck, CH van; Rutten, J.P.; Dejong, C.H.; Goor, H. van; Busch, O.R.; Gouma, D.J.

    2008-01-01

    BACKGROUND: Pancreas-preserving total duodenectomy (PPTD) was introduced as a replacement for pancreatoduodenectomy (PD) for familial adenomatous polyposis (FAP). This study analysed the results of PPTD in the Netherlands and reviewed the relevant literature. METHODS: All 26 patients who underwent P

  3. Seven-year survival after pancreaticoduodenectomy for early recurrent renal cell carcinoma involving the duodenum A case report.

    Science.gov (United States)

    Cannistra', Marco; Ruggiero, Michele; Bonaiuto, Elisabetta; Vaccarisi, Sebastiano; Naso, Agostino; Grande, Raffaele; Nardo, Bruno

    2015-12-29

    Il cancro del rene rappresenta la 13a forma di tumore a livello mondiale e risulta incrementata, dal punto di vista epidemiologico, di circa il 2% annuo nelle ultime due decadi. Studi recenti hanno mostrato un numero complessivo di 65,150 nuovi casi di cancro renale diagnosticato nei paesi occidentali e circa 13.680 morti. Il carcinoma a cellule renali rappresenta approssimativamente il 90% di tutte le forme di cancro renale e presenta un tasso di mortalità di circa 4 soggetti ogni 100000 abitanti per anno. Il carcinoma a cellule renali ha, spesso, un particolare corteo sintomatologico caratterizzato da dolore addominale, masse addominali ed ematuria. Caratteristico è il fatto che circa il 25-30% dei pazienti, all’atto della diagnosi, presentano già metastasti a distanza; un ulteriore 30-50%, inoltre, svilupperanno metastasi nel corso della malattia, anche dopo molti anni dall’operazione chirurgica di nefrectomia radicale. Il Cancro del Rene a Cellule Chiare metastatizza in genere per via linfatica, ematogena, transperitoneale o per invasione diretta, ed i siti di metastasi più comuni sono il polmone ed i linfonodi, seguiti dalle ossa e dal fegato. Tra tutte le possibili localizzazioni ai segmenti addominali, il duodeno rappresenta la sede meno colpita: quando coinvolta, viene, in genere, invasa la regione periampollare ed il bulbo del duodeno. Tali localizzazioni danno segno di sè, spesso tardivamente, con sanguinamenti (ematemesi) o ostruzioni del tratto gastrointestinale superiore (vomito). Il nostro caso clinico descrive un carcinoma a cellule renali che si presentava, dopo soli 5 mesi dalla nefrectomia radicale destra, con sanguinamento del tratto gastrointestinale superiore e metastasi a carico del duodeno, e che è stato trattato chirurgicamente con duodenocefalopancreasectomia. L’intervento chirurgico, noto per essere altamente demolitivo ed associato ad elevata morbilità, applicato a recidiva precoce di cancro, ha garantito un ottimo risultato per il paziente ed una migliore e più duratura sopravvivenza rispetto ai dati attualmente in letteratura.

  4. Uptake of /sup 75/Se-selenite by brush border membrane vesicles from chick duodenum stimulated by vitamin D

    Energy Technology Data Exchange (ETDEWEB)

    Mykkanen, H.M.; Wasserman, R.H.

    1989-02-01

    Brush border membrane vesicles were isolated from mucosal homogenates of duodena from normal, rachitic and vitamin D-treated rachitic chicks using a discontinuous sucrose gradient, and further purified by glycerol gradient centrifugation. In vitro uptake of 75Se-selenite by purified brush border membrane vesicles was studied using a rapid filtration technique. The time course of 75Se uptake was non-linear; rapid initial binding was followed by a gradual decrease in the rate of uptake until an equilibrium value was reached at 60-120 min. The initial binding at 36 s was not affected by selenite concentration in the incubation buffer, while the fractional rate of uptake between the 36 s and 2 min time periods was clearly lower with 1 mM Se than with 4-100 microM Se. 75Se uptake did not show any dependency on the external Na-gradient, nor could it be inhibited by other anions (arsenate, phosphate). Treatment of rachitic chicks either with cholecalciferol (500 Iu, 72 h) or with 1,25(OH)2-cholecalciferol (0.5 microgram given 16 h prior to isolation of the vesicles) significantly enhanced 75Se uptake. A threefold excess of mannitol in the outside buffer reduced 75Se uptake by vesicles from vitamin D-deficient and D-treated chicks 60% and 35% respectively, but had no effect on vesicles from vitamin D-treated chicks preloaded with 75Se. Neither saponin treatment nor excess cold selenite could release the label from the vesicles preloaded with 75Se. These data are compatible with the hypothesis that selenite easily crosses the brush border membrane into the intravesicular space and, once inside, is tightly bound by the membrane.

  5. Establishmentement of animal model with duodenum-stomach- esophageal reflux%十二指肠胃食管反流动物模型的构建

    Institute of Scientific and Technical Information of China (English)

    梁新生; 宋晨光; 袁红霞

    2012-01-01

    [objective] To improve the operation fashion of interrupted suture of "four walls and four stitches" and improve the establishment of animal models of deodenum-stomach-esophageal reflux. [Methods] The blank group(A) accepted no any special treatment while both the control(B) and the model(C) group received "four walls and four stitches" interrupted suture. Materials were obtained 5 days after modeling in group B while 10 days after that in C group. [Results] The survival rate of model group(83.4%) was significantly higher than that of control group (64.8%) (P<0.05). Both macroscopic characteristics and pathological changes of lower esophagus mucosa of rats in model group was significantly different from that of rats in control group (P<0.05). [Conclusion] The modified operating fashion in modeling can delay the survival rate and successfully induce reflex esophagitis and Barrett's esophagus.%[目的]改进“四壁四针”间断吻合术式,完善十二指肠胃食管反流大鼠模型的构建.[方法]空白组不作特殊处理,对照组和模型组都采用“四壁四针”间断吻合术式.对照组造模5d后取材,模型组10d后取材.[结果]模型组大鼠成活率(83.4%)较对照组(64.8%)差异有统计学意义(P<0.05),模型组大鼠食管下端黏膜肉眼表现较对照组差异有统计学意义(P<0.05),模型组大鼠食管下段病理改变与对照组差异有统计学意义(P<0.05).[结论]改进术式的模型在增加存活率的基础上成功的诱发了反流性食管炎及Barrett食管.

  6. Plasmacytoid Urothelial Carcinoma of the Urinary Bladder Metastatic to the Duodenum: A Case Report—Diagnostic Relevance of GATA3 Immunohistochemistry

    Directory of Open Access Journals (Sweden)

    Hermann Brustmann

    2017-01-01

    Full Text Available Plasmacytoid urothelial carcinoma (PUC of the urinary bladder is a rare and aggressive subtype of urothelial carcinoma. Its deceptive morphology is characterized by a discohesive growth of cells with plasmacytoid morphology. Since this tumor might be confused with plasmacytoma, lymphoma, or carcinoma variants, appropriate diagnosis in small biopsy samples could be challenging. This study reports the case of a 53-year-old man who presented with frequent nocturnal urgency, without hematuria. A transurethral bladder and a prostate resection specimen displayed infiltration of neoplastic cells in a spray-like discohesive pattern with occasional formation of small irregular nests and cord-like arrangements. The basic morphology of the tumor cells was plasmacytoid, with eccentric nuclei and eosinophilic cytoplasm. Tumor cells grew through the lamina muscularis mucosae, with splintering of the bladder wall musculature and infiltration of prostatic tissue. They displayed strong and diffuse nuclear reactivity for p53 and GATA3. Eight months after surgery, the patient experienced upper abdominal discomfort. A duodenal biopsy showed infiltration of plasmacytoid atypical cells strongly immunoreactive for GATA3, consistent with the previously diagnosed PUC. The patient died eleven months after the primary diagnosis of his PUC of tumor cachexia losing about 50% of his original body weight, furthermore, with ascites and intraperitoneal tumor spread.

  7. The absorption enhancement of norisoboldine in the duodenum of adjuvant-induced arthritis rats involves the impairment of P-glycoprotein.

    Science.gov (United States)

    Duan, Cong; Guo, Jiao-Mei; Dai, Yue; Xia, Yu-Feng

    2017-01-01

    Lindera aggregata (Sims) Kosterm root has been used in traditional Chinese medicine for the treatment of rheumatism palsy, dyspepsia and frequent urination for a long time. Norisoboldine, the main active constituent of this herb drug, possesses outstanding anti-arthritis activity. However, the in vivo disposition of norisoboldine is known to a limited extent, especially under the pathological condition of rheumatoid arthritis (RA). The aim of this study is to investigate whether and how the absorption of norisoboldine is altered in adjuvant-induced arthritis (AIA) rats. Comparative studies of the intestinal absorption of norisoboldine in normal and AIA rats at different pathological stages of the arthritis were performed using in situ single-pass intestinal perfusion, and the effects of an inhibitor of efflux proteins were also investigated. Norisoboldine was shown to be a substrate of P-glycoprotein (P-gp), as P-gp inhibitor verapamil markedly increased the permeability coefficient (Peff ) of norisoboldine by 88% in the intestine of normal rats. Compared with normal rats, AIA rats displayed increased Peff values of norisoboldine by 84% and 86% on day 5 and day 10 after the appearance of the secondary response of arthritis, respectively. Verapamil could eliminate the difference of intestinal absorption of norisoboldine between normal and AIA rats. Further studies showed that impaired expression and activity of P-gp in AIA rats play a decisive role in the absorption enhancement of norisoboldine. Notably, the impairment of P-gp function positively correlated with the severity of arthritis. Copyright © 2016 John Wiley & Sons, Ltd.

  8. 胃镜下切除十二指肠球部Brunner腺瘤1例%1 case of duodenum Brunner adenoma resection via gastroscope

    Institute of Scientific and Technical Information of China (English)

    张媛; 朴熙绪; 金仁顺; 朴东明

    2007-01-01

    @@ 1临床资料 患者男,57岁,农民.2个月前出现上腹痛饱胀不适进食后加重,到我院行胃镜检查示:十二指肠球部大弯侧见一1.2 cm×1.5 cm山田Ⅱ型息肉,表面糜烂,行内镜下高频电凝切除.切除肿物大小为1.0cm×1.5 cm送病理检查.病理检查示:肿物内见十二指肠腺增生,腺上皮形态呈立方低柱状或高柱状,核居中或居基底,分泌黏液,腺体呈小叶状分布,见图1~3.病理诊断:十二指肠Brunner腺瘤.术后患者住院观察给予洛赛克、止血敏、止血芳酸治疗,5 d后复查胃镜,见十二指肠球部大弯侧黏膜轻度隆起,表面形成浅表溃疡.

  9. Helicobacter pylori VacA, acting through receptor protein tyrosine phosphatase α, is crucial for CagA phosphorylation in human duodenum carcinoma cell line AZ-521

    Science.gov (United States)

    Yahiro, Kinnosuke; Yamasaki, Eiki; Kurazono, Hisao; Akada, Junko; Yamaoka, Yoshio; Niidome, Takuro; Hatakeyama, Masanori; Suzuki, Hidekazu; Yamamoto, Taro; Moss, Joel; Isomoto, Hajime; Hirayama, Toshiya

    2016-01-01

    ABSTRACT Helicobacter pylori, a major cause of gastroduodenal diseases, produces vacuolating cytotoxin (VacA) and cytotoxin-associated gene A (CagA), which seem to be involved in virulence. VacA exhibits pleiotropic actions in gastroduodenal disorders via its specific receptors. Recently, we found that VacA induced the phosphorylation of cellular Src kinase (Src) at Tyr418 in AZ-521 cells. Silencing of receptor protein tyrosine phosphatase (RPTP)α, a VacA receptor, reduced VacA-induced Src phosphorylation. Src is responsible for tyrosine phosphorylation of CagA at its Glu-Pro-Ile-Tyr-Ala (EPIYA) variant C (EPIYA-C) motif in Helicobacter pylori-infected gastric epithelial cells, resulting in binding of CagA to SHP-2 phosphatase. Challenging AZ-521 cells with wild-type H. pylori induced phosphorylation of CagA, but this did not occur when challenged with a vacA gene-disrupted mutant strain. CagA phosphorylation was observed in cells infected with a vacA gene-disrupted mutant strain after addition of purified VacA, suggesting that VacA is required for H. pylori-induced CagA phosphorylation. Following siRNA-mediated RPTPα knockdown in AZ-521 cells, infection with wild-type H. pylori and treatment with VacA did not induce CagA phosphorylation. Taken together, these results support our conclusion that VacA mediates CagA phosphorylation through RPTPα in AZ-521 cells. These data indicate the possibility that Src phosphorylation induced by VacA is mediated through RPTPα, resulting in activation of Src, leading to CagA phosphorylation at Tyr972 in AZ-521 cells. PMID:27935824

  10. Plasmacytoid Urothelial Carcinoma of the Urinary Bladder Metastatic to the Duodenum: A Case Report—Diagnostic Relevance of GATA3 Immunohistochemistry

    Science.gov (United States)

    2017-01-01

    Plasmacytoid urothelial carcinoma (PUC) of the urinary bladder is a rare and aggressive subtype of urothelial carcinoma. Its deceptive morphology is characterized by a discohesive growth of cells with plasmacytoid morphology. Since this tumor might be confused with plasmacytoma, lymphoma, or carcinoma variants, appropriate diagnosis in small biopsy samples could be challenging. This study reports the case of a 53-year-old man who presented with frequent nocturnal urgency, without hematuria. A transurethral bladder and a prostate resection specimen displayed infiltration of neoplastic cells in a spray-like discohesive pattern with occasional formation of small irregular nests and cord-like arrangements. The basic morphology of the tumor cells was plasmacytoid, with eccentric nuclei and eosinophilic cytoplasm. Tumor cells grew through the lamina muscularis mucosae, with splintering of the bladder wall musculature and infiltration of prostatic tissue. They displayed strong and diffuse nuclear reactivity for p53 and GATA3. Eight months after surgery, the patient experienced upper abdominal discomfort. A duodenal biopsy showed infiltration of plasmacytoid atypical cells strongly immunoreactive for GATA3, consistent with the previously diagnosed PUC. The patient died eleven months after the primary diagnosis of his PUC of tumor cachexia losing about 50% of his original body weight, furthermore, with ascites and intraperitoneal tumor spread. PMID:28255490

  11. Relationship of membrane-bound sulfhydryl groups to vitamin D-stimulated uptake of ( sup 75 Se)Selenite by the brush border membrane vesicles from chick duodenum

    Energy Technology Data Exchange (ETDEWEB)

    Mykkanen, H.M.; Wasserman, R.H. (Cornell Univ., Ithaca, NY (USA))

    1990-08-01

    The uptake of selenite by purified brush border membrane vesicles isolated from duodena of rachitic or vitamin D-treated chicks was studied by using radioactive selenite and a rapid filtration technique. Cholecalciferol treatment (500 IU at 72 h) significantly enhanced selenite uptake, a response that decreased when the vesicles were stored at room temperature for 2.5 h prior to the uptake measurement. Preincubation of the vesicles in 1.0 mmol/L H2O2 reduced (75Se)selenite uptake, indicating the involvement of oxidizable groups in the uptake reaction. Iodoacetic acid (IAA), a sulfhydryl-blocking reagent, at 1-2 mmol/L concentration eliminated the difference in selenite uptake due to cholecalciferol and had no effect on vesicles from rachitic animals. A higher concentration of IAA (10 mmol/L) enhanced selenite uptake manyfold and increased the absolute difference due to cholecalciferol treatment. Single intravenous doses of 100 IU cholecalciferol, 100 IU ergocalciferol, or 0.1 micrograms 1,25-dihydroxycholecalciferol also stimulated selenite uptake, suggesting a general response to vitamin D compounds. Normal animals given a single dose of 1,25-dihydroxycholecalciferol 12 h prior to killing also responded. Treatments that enhanced the uptake of (75Se)selenite also increased the amount of membrane-bound sulfhydryl groups, suggesting the involvement of membrane-bound sulfhydryl groups in the vitamin D response. A significant increase in selenite uptake by intravenous 1,25-dihydroxycholecalciferol occurred within 10 min. This rapid effect provides a new tool to probe early biochemical effects of vitamin D on intestinal epithelium.

  12. Cryofixation, ultracryomicrotomy, and X-ray microanalysis of enterocytes from chick duodenum: Vitamin-D-induced formation of an apical tubulovesicular system

    Energy Technology Data Exchange (ETDEWEB)

    Davis, W.L.; Hagler, H.K.; Jones, R.G.; Farmer, G.R.; Cooper, O.J.; Martin, J.H.; Bridges, G.E.; Goodman, D.B. (Baylor Univ. Medical Center, Dallas, TX (USA))

    1991-02-01

    New methods of tissue preparation were developed to study the morphology and distribution of calcium ions in duodenal enterocytes from normal, rachitic, and vitamin D-replete (either cholecalciferol (CC) or 1,25-dihydroxycholecalciferol (1,25-DHCC) treated) chicks. Frozen hydrated sections were prepared from cryofixed tissues by ultracryomicrotomy at -125 degrees C. Sections were subsequently freeze-dried by increasing the temperature to -100 degrees C. The latter temperature was maintained throughout both the structural and elemental analyses. In cells from normal, rachitic, and vitamin D-treated (CC) animals the brush border from lanthanum-infused tissues was electron dense and calcium-lanthanum positive by x-ray analysis. In the absence of lanthanum, i.e., sucrose-infused duodena, the microvilli were still calcium positive. In the terminal web region of normal and CC-treated enterocytes, numerous, apparently interconnected, tubules and vesicles were seen. Vacuole-like structures were also seen. Such structures were especially prominent in the enterocytes from the vitamin-treated (CC) animals. Except for the vacuoles, the tubules and vesicles were electron dense in the lanthanum-infused duodena, and clear in sucrose-infused tissues. In both instances, the structures were calcium positive. Similar, but even larger structures were seen below the terminal web. Here however, the tubules and vesicles seemed to be organized into multiple complex interconnecting networks, i.e., tubulo-vesicular complexes. Both the tubules and the vesicles seemed to be interconnected via smaller channel-like entities. The extensiveness of this structure was better appreciated in the enterocytes from lanthanum-infused tissues, where it appeared similar in structure and complexity to an en face view of the sarcoplasmic reticulum of skeletal muscle.

  13. Effects of acute administration of omeprazole or ranitidine on basal and vagally stimulated gastric acid secretion and alkalinization of the duodenum in anaesthetized cats.

    Science.gov (United States)

    Fändriks, L; Jönson, C

    1990-02-01

    Experiments were performed on acutely vagotomized cats during chloralose anaesthesia. In order to avoid sympathoadrenergic influences, the adrenal glands were ligated and the splanchnic nerves were cut bilaterally in all animals. The gastric lumen was perfused with saline and the H+ secretion was calculated from pH measurements in the perfusate. HCO3- secretion by the duodenal mucosa was titrated in situ. Omeprazole (4 mg kg-1 i.v., dissolved in PEG400, 40% w/v) did not influence basal or vagally induced HCO3- secretions, but inhibited by about 80% the H+ secretory response induced by electric vagal stimulation. Acute administration of ranitidine (5 mg kg-1 i.v.) transiently lowered arterial pressure, an effect which was followed by a sustained compensatory tachycardia. Ranitidine raised basal duodenal HCO3- secretion by 50% and inhibited vagally induced gastric H+ secretion by about 70%, whereas vagally induced HCO3- secretion was not influenced. The results suggest that vagal nerve stimulation raises the duodenal bicarbonate secretion via a mechanism independent of the level of gastric H+ secretion.

  14. Assessment of genotoxic potential of Cr(VI) in the mouse duodenum: an in silico comparison with mutagenic and nonmutagenic carcinogens across tissues.

    Science.gov (United States)

    Thompson, Chad M; Gregory Hixon, J; Proctor, Deborah M; Haws, Laurie C; Suh, Mina; Urban, Jonathan D; Harris, Mark A

    2012-10-01

    In vitro studies on hexavalent chromium [Cr(VI)] indicate that reduced forms of this metal can interact with DNA and cause mutations. Recently, Cr(VI) was shown to induce intestinal tumors in mice; however, Cr(VI) elicited redox changes, cytotoxicity and hyperplasia - suggesting involvement of tissue injury rather than direct mutagenesis. Moreover, toxicogenomic analyses indicated limited evidence for DNA damage responses. Herein, we extend these toxicogenomic analyses by comparing the gene expression patterns elicited by Cr(VI) with those of four mutagenic and four nonmutagenic carcinogens. To date, toxicogenomic profiles for mutagenic and nonmutagenic duodenal carcinogens do not exist, thus duodenal gene changes in mice were compared to those elicited by hepatocarcinogens. Specifically, duodenal gene changes in mice following exposure to Cr(VI) in drinking water were compared to hepatic gene changes previously identified as potentially discriminating mutagenic and nonmutagenic hepatocarcinogens. Using multivariate statistical analyses (including logistic regression classification), the Cr(VI) gene responses clustered apart from mutagenic carcinogens and closely with nonmutagenic carcinogens. These findings are consistent with other intestinal data supporting a nonmutagenic mode of action (MOA). These findings may be useful as part of a full weight of evidence MOA evaluation for Cr(VI)-induced intestinal carcinogenesis. Limitations to this analysis will also be discussed.

  15. Acute relaxation of mouse duodenum [correction of duodenun] by estrogens. Evidence for an estrogen receptor-independent modulation of muscle excitability.

    Science.gov (United States)

    Díaz, Mario; Ramírez, Cristina M; Marin, Raquel; Marrero-Alonso, Jorge; Gómez, Tomás; Alonso, Rafael

    2004-10-06

    17-beta-Estradiol, the stereoisomer 17-alpha-estradiol and the synthetic estrogen diethylstilbestrol (DES), all caused a rapid (verruculogen. The effects of BAY-K8644 and K(+) channel blockers were synergistic, and allowed relaxed tissues to recover spontaneous activity and basal tone. We hypothesize that the rapid non-genomic spasmolytic effect of estrogens on mouse duodenal muscle might be triggered by an estrogen-receptor-independent mechanism likely involving activation of tetraethylamonium- and 4-aminopyridine-sensitive K(+) channels and inhibition of L-type Ca2(+) channels on the smooth muscle cells.

  16. Diagnosing and treating experience of newborn duodenum intestinal obstruction 123 cases%新生儿十二指肠梗阻123例诊疗研究

    Institute of Scientific and Technical Information of China (English)

    侯立功; 王献良; 郜向阳; 侯广军; 黄敏

    2011-01-01

    Objective To investigate the diagnosis, treatment and pathology of congenital duodenal obstruction. Methods Totally 123 cases of neonatal duodenal obstruction in children in the preoperative diagnosis, pathology, and treatment were analyzed retrospectively. Results In 123 patients with duodenal obstruction in children, 56 cases of intestinal malrotation (45%);Duodenal atresia and stenosis of 27cases (21%), in which the bag- type membrane-type and 13 cases of duodenal stenosis in 7 cases,9 cases of duodenal atresia; Ring obstruction caused by the pancreas in 11 cases (8%). With malformations: the merger of the group suffered 11 cases of meconium peritonitis, volvulus necrosis in 4 cases, 4cases of gastroschisis, omphalocele in 3 cases, Meckel' s diverticulum in 4 cases, 18 cases of congenital heart disease. 123 cases of children in 117 patients survived ( survival rate95.12% ), 3 patients died in hospital, after surgery in 5 cases gave up treatment within 1 ~ 8d, 11 cases of annular pancreas and 7 cases of intestinal stenosis in children all survived. Complications include adhesive intestinal obstruction ( 13 cases), pneumonia (67 cases), anastomotic leakage (5 cases), small stress ulcer ( 1 case), wound dehiscence (11 cases), neonatal hard swelling disease (22cases). Conclusions B ultrasound prenatal fetal stomach and 12 found to expand or fetal polyhydramnios should consider the possibility of high intestinal obstruction;After birth bilious vomiting is a common characteristic of the symptoms ;Preoperative X - ray visible double - bubble or three bubble characteristics, upper gastrointestinal contrast agent visible block and (or) through difficulties, is an effective method of diagnosis of duodenal obstruction ;Early diagnosis, rational selection of operative method, to avoid misdiagnosis, strengthen perioperative and intravenous high- nutrition is the key to improve the cure rate.%目的 探讨新生儿先天性十二指肠梗阻的诊断和治疗方法及病理类型.方法 对123例新生儿十二指肠梗阻患儿的术前诊断、病理类型及治疗方法进行回顾性分析.结果 123例十二指肠梗阻患儿中,肠旋转不良56例(45%);十二指肠闭锁与狭窄27例(21%),其中隔膜型及风袋型13例,十二指肠狭窄7例,十二指肠闭锁9例;环型胰腺造成的梗阻11例(8%).伴发畸形:本组患儿中合并胎粪性腹膜炎11例,肠扭转坏死4例,腹裂4例,脐膨出3例,美克尔憩室4例,先天性心脏病18例.123例患儿中117例存活(存活率95.12%),3例医院内死亡,5例手术后1-8d内放弃治疗,11例环型胰腺及7例肠狭窄患儿全部存活.术后并发症主要包括粘连性肠梗阻(13例)、肺炎(67例)、吻合口瘘(5例)、应激性小肠溃疡(1例)、切口裂开(11例)、新生儿硬肿症(22例).结论 孕妇产前B超检查若发现胎儿胃及十二扩张或羊水过多都应该考虑胎儿高位肠梗阻的可能;生后胆汁性呕吐为常见的具有特征性的症状;术前x线平片可见双气泡或三气泡特征,上消化道造影可见造影剂阻滞和(或)通过困难,是诊断十二指肠梗阻的有效方法;早期诊断,合理选择手术方式,避免漏诊,加强围手术期处理及静脉高营养是提高治愈率的关键.

  17. Solitary Peutz-Jeghers type hamartomatous polyps in the duodenum are not always associated with a low risk of cancer: two case reports

    Directory of Open Access Journals (Sweden)

    Koide Tomoko

    2011-06-01

    Full Text Available Abstract Introduction A hamartomatous polyp without associated mucocutaneous pigmentation or a family history of Peutz-Jeghers Syndrome is diagnosed as a solitary Peutz-Jeghers type hamartomatous polyp. As compared with Peutz-Jeghers Syndrome, Peutz-Jeghers type hamartomatous polyps are diagnosed with a lower risk of cancer and are regarded as a different disorder. Case presentation In case one, we describe an 84-year-old Japanese man with a 14 mm duodenal polyp. Endoscopic mucosal resection was performed and histological examination showed findings suggestive of a hamartomatous polyp with a focus of well-differentiated adenocarcinoma. In case two, we describe a 76-year-old Japanese man who had been treated for prostate, rectal and lung cancer. Upper gastrointestinal endoscopy revealed a duodenal polyp measuring 15 mm in diameter. Endoscopic mucosal resection was performed, and histological examination showed findings suggestive of a hamartomatous polyp. Liver and thyroid cancers were found after the endoscopic treatment. Conclusion Although duodenal solitary hamartomatous polyps are associated with a lower risk of cancer, four patients, including our cases, have been diagnosed with cancerous polyps. Patients with duodenal solitary hamartomatous polyps should be treated by endoscopic or surgical resection and need whole-body screening.

  18. Ca2(+)-channel agonist BAY K8644 mimics 1,25(OH)2-vitamin D3 rapid enhancement of Ca2+ transport in chick perfused duodenum

    Energy Technology Data Exchange (ETDEWEB)

    de Boland, A.R.; Nemere, I.; Norman, A.W. (Univ. of California, Riverside (USA))

    1990-01-15

    To further understand the molecular mechanism by which 1,25(OH)2-vitamin D3 (1,25(OH)2D3) rapidly stimulates intestinal calcium transport (termed transcaltachia), the effect of the calcium channel agonist BAY K8644 was studied in vascularly perfused duodenal loops from normal, vitamin D-replete chicks. BAY K8644, 2 mu M, was found to stimulate {sup 45}Ca{sup 2+} transport from the lumen to the vascular effluent to the same extent as physiological levels of 1,25(OH)2D3. The sterol and the Ca{sup 2+} channel agonist both increased {sup 45}Ca{sup 2+} transport 70% above control values within 2 min and 200% after 30 min of vascular perfusion. The effect of the Ca{sup 2+} channel agonist was dose dependent. Also, 1,25(OH)2D3-enhanced transcaltachia was abolished by the calcium channel blocker nifedipine. Collectively, these results suggest the involvement of 1,25(OH)2D3 in the activation of basal lateral membrane Ca{sup 2+} channels as an early effect in the transcaltachic response.

  19. 笼养成年蛋鸡十二指肠中乳酸菌的分离与鉴定%Isolation and Identification of Lactic Acid Bacteria in Duodenum of Laying Hens Fed in Cage

    Institute of Scientific and Technical Information of China (English)

    王秋菊; 崔一喆; 刘胜军; 周瑞进

    2013-01-01

      Lactobaccillus isolated from duodenal contents of laying hens fed in cage were identified by Gram staining,observing strain morphous,and biochemistry fermentation. The results showed that totally 16 strains were gram staining positive,all strains were assayed by antibacterial activity to select better ones for identification by biochemistry fermentation with different sugar. In all, 7 Lactic acid bacteria were identified, which were Lactobacillus bulgaricus, salt tolerance of Lactobacillus, Lactobacillus amylophilus,acidophilus lactobacillus, Food starch Lactobacillus,Lactobacillus casei heat -resistant subsp,Leuconostoc mesenteroides subsp cremoris.%  试验从笼养蛋鸡十二指肠内容物中分离乳酸菌,经过革兰氏染色、菌落形态观察和糖发酵生化鉴定乳酸菌种属。结果显示,分离出16株革兰氏阳性菌,经抑菌试验选出7株乳酸菌,分别鉴定为乳杆菌保加利亚种,耐盐乳杆菌,嗜淀粉乳杆菌,坎氏乳杆菌,食淀粉乳杆菌,干酪乳杆菌耐热亚种,肠膜明串球菌乳脂亚种。

  20. Nursing coordination of 2 cases undergoing robot auxiliary duodenum preserving pancreatic head resection%机器人辅助保留十二指肠的胰头切除术的手术配合

    Institute of Scientific and Technical Information of China (English)

    郭健; 韦欣; 钱蒨健

    2011-01-01

    @@ 1972年,Beger等首先报道了保留十二指肠的胰头亚全切除术治疗慢性胰腺炎[1].该手术在距十二指肠2 cm处切断胰头,沿十二指肠保留一部分胰腺组织以防损伤沿十二指肠走行的胰十二指肠动脉弓;在门静脉前方胰颈处离断胰腺,切除部分胰头,切断空肠后分别行空肠-胰体尾吻合与空肠-残余胰头吻合重建消化道.随后Beger等又基于该手术创建了保留十二指肠的胰头全切术.

  1. Effect of Da Chengqi Decoction on Rabbits' Duodenum Muscle Activity in Vitro%大承气汤对兔离体十二指肠平滑肌活动的影响

    Institute of Scientific and Technical Information of China (English)

    张启荣; 朱克刚; 彭吉霞; 王星文

    2011-01-01

    目的:探讨中药大承气汤与大黄、厚朴、芒硝、枳实各单味药对兔离体十二脂肠平滑肌活动的影响.方法:采用Magnus实验装置,使用BL-410生物信号记录系统,描记中国白兔离体十二指肠平滑肌收编、舒张活动曲线;5分钟作为对照,然后观察药物对其作用3分钟后曲线变化,并与对照组比较.结果:给大承气汤煎剂后,十二指肠平滑肌收缩幅度、频率、张力明显增加,与对照组比较有显著性差异(P<0.05或P<0.01);大黄、厚朴、芒硝、枳实各单味药煎剂对其影响有明显不同,其中大黄、芒硝有明显增加十二指肠平滑肌收缩幅度、频率、张力的作用(P<0.05或P<0.01),而厚朴、枳实降低十二指肠平滑肌收缩幅度、频率、张力(P<0.05或P<0.01);枳实活动抑制率是厚朴的1.37倍.结论:大承气汤、大黄、芒硝有促进肠平滑肌运动作用.

  2. Influence of Glutamine Supplementation on Duodenum Morphological Structure in Broilers%外源性谷氨酰胺对肉仔鸡十二指肠组织结构的影响

    Institute of Scientific and Technical Information of China (English)

    戴四发; 李如兰; 闻爱友; 王立克

    2007-01-01

    研究外源性谷氨酰胺(Gln)对肉仔鸡早期(0~4周龄)十二指肠形态结构的影响.288只1日龄健康AA鸡随机分成3组,每组3个重复.Ⅰ组为对照组,饲喂基础日粮,Ⅱ组和Ⅲ组为试验组,分别饲喂基础日粮中添加0.4%和0.8%Gln的试验日粮.分别于第1~4周龄时取十二指肠在光学显微镜下进行组织学观察.外源性Gln对各试验周龄十二指肠的绒毛高度、绒毛宽度、隐窝深度、粘膜肌层厚度、肌层厚度和浆膜层厚度的变化均有不同程度的影响.0.4%浓度Cln增加了4周龄时的绒毛高度和粘膜肌层厚度(P<0.01),减少了4周龄时的绒毛宽度、隐窝深度和肌层厚度(P<0.05或P<0.01).0.8%浓度Gln增加了3周龄内的绒毛高度(P<0.05或P<0.01)、2周龄内的肌层厚度(P<0.05或P<0.01)、3周龄时的绒毛宽度(P<0.01)和 4周龄时的浆膜层厚度(P<0.01),减小了1周龄时的隐窝深度(P<0.01)、3周龄内的粘膜肌层厚度(P<0.05或P<0.01)和4周龄时肌层厚度(P<0.05).

  3. 腹腔镜十二指肠溃疡穿孔修补术的应用体会%The Appy Experience of Repair Operation of Duodenum Ulcer Perforation with Celioscope

    Institute of Scientific and Technical Information of China (English)

    黄焕基; 林妙承

    2005-01-01

    目的探讨腹腔镜十二指肠溃疡穿孔修补的效果及应用价值.方法收集我院2004年7月~2005年2月间用腹腔镜行十二指球部溃疡穿孔修术的13例.结果手术均获成功,平均手术时间100min,平均进食时间4.5d,平均住院时间为6d,未发生并发症.结论用腹腔镜行十二指肠球部溃疡穿孔修补是安全有效的治疗方法,具有痛苦小、损伤轻,康复快、脏器干扰小、腹腔冲洗彻底、住院时间短、患者乐意接受等优点,值得临床推广应用.

  4. Annular pancreas (image)

    Science.gov (United States)

    Annular pancreas is an abnormal ring or collar of pancreatic tissue that encircles the duodenum (the part of the ... intestine that connects to stomach). This portion of pancreas can constrict the duodenum and block or impair ...

  5. THE EFFECT OF GnRH-A ON THE FUNCTION OF SOMATOSTATIN CELLS IN STOMACH AND DUODENUM OF RATS%胃腔内促性腺激素释放激素类似物对大鼠胃和十二指肠生长抑素细胞功能的影响

    Institute of Scientific and Technical Information of China (English)

    刘晓宁; 黄威权; 高彬

    2004-01-01

    目的研究胃腔内促性腺激素释放激素类似物(GnRH-A)对大鼠胃和十二指肠生长抑素免疫阳性细胞及血液、胃液中生长抑素含量的影响.方法应用免疫组织化学ABC和ELISA方法分别检测大鼠胃和十二指肠生长抑素阳性细胞的密度及血液、胃液中生长抑素的含量. 结果胃腔注射GnRH类似物后,胃壁内单位面积生长抑素阳性细胞数为26.6±3.893,与对照组48.3±6.019相比具有差异(P<0.01),十二指肠单位面积生长抑素阳性细胞数为51.7±2.214,与对照组58.5±4.454相比具有差异(P<0.01).血液中生长抑素ELISA检测的A值为0.15±0.018,与对照组0.27±0.036相比具有差异(P<0.01),胃液中A值为0.32±0.049,与对照组1.022±0.369相比具有差异(P<0.01). 结论外分泌的GnRH对大鼠胃和十二指肠中生长抑素的分泌起显著的抑制作用.

  6. Effects of 5-HT, glutamate, histidine Leu-Enk on contraction of longitudinal muscle of rabbit duodenum%五羟色胺、谷氨酸、组氨酸、脑啡肽在家兔离体十二指肠纵肌收缩中的作用

    Institute of Scientific and Technical Information of China (English)

    张昆茹

    2000-01-01

    以家兔离体十二指肠纵肌方向的张力为指标,观察了五羟色胺(5-HT)、谷氨酸(Glutamate)、组氨酸(Histidine)、脑啡肽(Leu-Enk)对十二指肠的作用.实验表明,5-HT对十二指肠纵肌有明显的兴奋作用,5-HT可兴奋乙酰胆碱(Ach)能神经元,Ach又可抑制5-HT能神经元的活动;Glumatame对十二指肠纵肌的活动有明显的抑制作用;Histidine对十二指肠纵肌的作用与肠的机能状态有关;Leu-Enk对十二指肠纵肌活动有抑制作用,纳洛酮(Naloxone)可以阻断这种作用.

  7. Expression of PCNA,BCL-2 and BMP2/4 in diagnosis and prognosis of adenocarcinoma of the duodenum value%PCNA、BCL-2及 BMP2/4的表达对十二指肠腺癌的诊断意义及预后价值

    Institute of Scientific and Technical Information of China (English)

    李忠辉; 张晋冀; 石书玮; 邰智慧

    2016-01-01

    Objective To observe the expression of PCNA,BCL-2 and BMP2/4,in patients with duodenal adenocar-cinoma,to further explore the clinical significance of PCNA,BCL-2 and BMP2/4 in the diagnosis and prognosis.Meth-ods From 2005 January to 2014 November in our hospital check biopsy diagnosed 45 cases of duodenal adenocarcinoma patients,according to the Joint Committee American cancer AJCC staging criteria above,the patients were divided into group Ⅰ(7 cases),group Ⅱ(16 cases),group Ⅲ(13 cases)and IV (9 cases),using immunohistochemical staining SP method,the expression of PCNA,BCL-2 and BMP2/4detection in patients with biopsy is different,and the control group (45 cases)were analyzed by statistical software,calculation,the relationship between the expression of further a-nalysis of above factors and duodenal adenocarcinoma.Results The expression level of PCNA,BCL-2 and BMP2/4 in patients with duodenal adenocarcinoma,increased significantly compared with the control group (P <0.05),Ⅰ,Ⅱ,Ⅲ,Ⅳ,the difference between the four groups was significantly(P <0.05).Conclusion Expression of PCNA,BCL-2 and BMP2/4 of duodenal adenocarcinoma tissues is closely related to the disease,at the same time with the biological behav-ior and pathology of the disease the academic stages of relevance,it has certain guiding significance for the diagnosis of duodenaladenocarcinoma and prognosis.%目的:观察十二指肠腺癌患者体内 PCNA、BCL-2及 BMP2/4的表达情况,进一步探讨 PCNA、BCL-2及BMP2/4在该疾病诊断及预后中的临床意义。方法选取2005年1月至2014年11月间在我院检查取组织活检确诊为十二指肠腺癌患者45例,根据美国癌症联合委员会 AJCC 的分期标准,将以上患者分为Ⅰ组(7例)、Ⅱ组(16例)、Ⅲ组(13例)以及Ⅳ组(9例),采用免疫组织化学染色 SP 法,检测不同患者活检组织中的 PCNA、BCL-2及BMP2/4的表达情况,并与对照组(45例)进行对照分析,通过统计学软件计算,进一步分析以上因子的表达与十二指肠腺癌的关系。结果十二指肠腺癌患者体内 PCNA、BCL-2及 BMP2/4的表达水平与对照组比较升高明显(P <0.05),Ⅰ、Ⅱ、Ⅲ、Ⅳ四组比较差异明显(P <0.05)。结论十二指肠腺癌组织中 PCNA、BCL-2及 BMP2/4的表达与该类疾病密切相关,同时与该类疾病的生物学行为及病理学分期具有相关性,对诊断十二指肠腺癌及判断预后有一定指导意义。

  8. Treatment of malignant stenosis of stomach 、duodenum and jejunum through peroral implantion of metal stents: Report of 67 cases%经口放置金属支架治疗胃、十二指肠及空肠恶性狭窄67例

    Institute of Scientific and Technical Information of China (English)

    茅爱武; 杨仁杰; 刘寺义; 高斌; 程英升; 高中度

    2001-01-01

    目的 探讨经口放置胃肠道内支架的方法,评价金属支架在治疗胃、十二指肠及空肠恶性狭窄中的作用。方法 胃、十二指肠及近端空肠恶性狭窄病例67例。在X线监视下通过递送导管或胃镜经口送入超滑导丝并使之过胃、十二指肠狭窄或阻塞段;经交换导管替换软头特硬导丝后由硬导丝引入附镍钛合金支架之套管式推送器将支架送入狭窄段释放。对完全阻塞使用胃镜仍不能将导丝送入者则经胃镜用微波或热极烧灼形成小通道后再送入导丝引入支架(5例)。结果 67例中有64例经97例次操作,成功地放置了83个支架。支架置于胃体及幽门部18个,胃肠吻合口17个,十二指肠降段和水平段44个,近端空肠4个。另3例经5次操作放置支架未成功。支架置入病例临床症状均获缓解(59例)或明显改善(5例)。术后1个月复查支架通畅率97.5%,有4例共5个支架分别于支架置入后16~61d移位脱落,其中1个经口取出、3个自行经肛门排出、1个滞留于回盲部但均未出现并发症。结论 经口放置金属支架能有效缓解胃、十二指肠及空肠梗阻,为提高晚期肿瘤患者的生活质量、延长其生存时间,提供了姑息性治疗的方法%Objective To explore methods of remote implanting gastointesinal inner stent in digestive tract and evaluate the value of metal inner-stent implantation in the treatment of malignant gastro-duodenal jejunal stenosis. Methods There were 67 cases with malignant gastro-duodenal and proximal jejunal stenosis, age ranged from 20 to 86 years. Under X-ray monitoring super-slip guide wire was introduced in by conveying catheter or gastroscope, passing through gastro-duodenal stenosis or obstruction. For those of complete obstruction or failure for the guide-wire to pass, we used microwave or heat-node to build fine path first, and then we inserted guidewire to pass through with stent implanted. Results 64 cases of the whole group were operated for 95 times, 83 metal stents were implanted successfully. Among them, 18 times in gastric corpus and pyloric part,17 times in gastro-intestinal anastomosis.44 times in duodenal descending and horizontal segment,4 times in proximal jejunum, 59 cases had clinical symptomatic remission ,and 5 cases had much improvement in general condition. One month postoperative examination showed stent passing rate as 97.5%. Five stents in 4 cases revealed migration from 16 to 61 days after implantation. One stent was taken out through oral cavity,other three stents through anus and another one stayed at ileocecal region without any complication.Conclusion Peroral implantation of metal stents alleviated malignant gastro-duodenal and jejunal stenosis providing palliative treatment, raising quality of life and prolonging survival time in advanced cancer patients.

  9. Effects of Hypoxia on the Inflammatory Reaction and Iron Absorption in Duodenum of Rats during Strenuously Exercise%大强度运动后低氧对大鼠炎症反应和十二指肠铁吸收蛋白的影响

    Institute of Scientific and Technical Information of China (English)

    王海涛; 李海洲; 李立卓; 王金霞; 吕军; 刘玉倩

    2013-01-01

    目的:研究低氧对大鼠炎症反应和肠铁吸收相关蛋白的影响,阐明低氧影响机体铁状态的调节机制.方法:24只雄性Wistar大鼠随机分为安静对照组(C组)、大强度运动组(E组)和运动后低氧恢复组(HE组).E组和HE组进行递增负荷跑台运动,坡度0,速度30 m/min,6d/周,前2周1次/天,后2周2次/天,共4周.训练第1次为1 min,每次递增2 min.HE组每天运动后在13.6%O2的低氧舱中休息,8 h/d.检测各组血清铁,ELISA法检测血清铁调素(hepcidin)和白介素6(IL-6),Western Blot法检测十二指肠铁转运蛋白:血红素转运体1 (Heme transport protein1,HCPl)、二价金属离子转运蛋白1(divalent metal transporter 1,DMT1)和膜铁转运蛋白1(Ferroportin 1,FPN1)表达.结果:(1)HE组血清铁和Hb显著高于C组和E组(P<0.01,P<0.05),血清hepcidin、IL-6、白细胞数显著低于E组(P<0.05);而E组血清铁和Hb显著低于C组(P< 0.05,P<0.01).(2)HE组HCP1、DMT1、FPN1均显着高于C组和E组(P<0.01,P<0.05).结论:适度低氧降低运动机体炎症反应,减少肝分泌hepcidin,促进肠铁吸收,改善机体铁状态,预防运动性铁缺乏.

  10. 保留十二指肠的胰头切除术与胰十二指肠切除术治疗胰头肿块型胰腺炎的Meta分析%A meta-analysis of surgery treatment of chronic pancreatitis with an inflammatory mass in the head of pancreas: duodenum-preserving pancreatic head resection versus pancreatoduodenectomy

    Institute of Scientific and Technical Information of China (English)

    蒋康怡; 吴柯; 廖玉平; 涂兵

    2014-01-01

    Objective To compare the safety and effectiveness of DPPHR with PPPD/PD for treating chronic pancreatitis with an inflammatory mass in the head of pancreas.Methods The relative data bases such as Medline,EMBase,Biosis,COCHRANE Library,Science Citation Index,SinoMed,Chinese Journal Full-text Database,Wangfang,CNKI were searched systematically,researchers selected randomized controlled trials(RCT) and prospective clinical controlled trials(CCT).The assessment of the bias risk of the included trials was according to the assessing tools suggested by Cochrane Handbook 5.1.The Review Manage 5.2 was used to perform the statistical analysis.Results In total,5 RCTs and 2 CCTs were included,381 patients involved.Comparing with PPPD/PD procedure,DPPHR has no significant difference in terms of the mortality of perioperative period (RD =0.01,P =0.51),the incidence of bleeding (RD =-0.01,P =0.72),pancreatic fistula (RD =-0.01,P =0.59) and delayed gastric emptying (RD =-0.15,P =0.10),the ration of complete pain relief after operation(RR =1.06,P =0.32) and the score of global quality of life(WMD =10.31,P =0.19).While DPPHR had significant superiorities in terms of the total morbidity of perioperative period (RR =0.60,P =0.008),the duration of the operations (WMD =-71.60,P =0.03),the postoperative hospitalization duration (WMD =-3.95,P < 0.01),weight gain (WMD =3.68,P < 0.01),occupational rehabilitation after the operations (RR =1.38,P =0.008).Conclusions In terms of reducing the morbidity of perioperative period,shortening the duration of the operations and the postoperative hospitalization duration,weight gain,occupational rehabilitation after the operations,the DPPHR is more favorable for improving patients' life qualities comparing with PPPD/PD.%目的 探讨保留或不保留幽门的胰十二指肠切除术(PPPD/PD)与保留十二指肠的胰头切除术(DPPHR)治疗胰头肿块型胰腺炎的安全性和有效性.方法 系统检索Medline数据库、EMBase数据库、Biosis数据库、COCHRANELibrary、Science Citation Index数据库、中国生物医学文献数据库、中国期刊全文数据库、万方数据库、维普中文科技期刊数据库,选取随机对照试验和前瞻性临床对照试验,参考Cochrane Handbook5.1偏倚风险评估工具评估偏倚风险.应用Review Manager 5.2对两类术式安全性和有效性相关参数进行统计分析.结果 共纳入7篇文献,共计381例患者.DPPHR组与PPPD/PD组比较,在围术期死亡率(RD =0.01,P=0.51)、术后出血(RD=-0.01,P=0.72)、胰瘘(RD=-0.01,P=0.59)、胃排空延迟(RD=-0.15,P=0.10)、完全腹痛缓解(RR=1.06,P=0.32)、总体生活质量评分(WMD=10.31,P=0.19)等方面二者差异无统计学意义;在围术期总并发症发生率(RR =0.60,P=0.008)、手术时间(WMD=-71.60,P=0.03)、术后住院时间(WMD=-3.95,P<0.01)、体重增加(WMD=3.68,P<0.01)及工作恢复(RR=1.38,P=0.008)等方面DPPHR组优于PPPD/PD组,差异有统计学意义.结论 DPPHR在减少术后并发症、缩短手术及住院时间、体重恢复及工作恢复等方面优于PPPD/PD,有利于提高胰头肿块型慢性胰腺炎患者的生活质量.

  11. Biomechanical behavior study of dog's small intestines

    Institute of Scientific and Technical Information of China (English)

    陈柏; 周银生; 穆晓枫

    2002-01-01

    The biomechanical behavior of dog's duodenum and jejunum were studied and a formulation of the stress-strain relation is presented in this paper. The results obtained indicated that the exponential coefficient α and the incremental duodenum of the elastic modulus are both larger than those of the jejunum. It means that the duodenum is more deformable than the jejunum. The experimental results of this work provide basal data for kinematics study of a robotic endoscope.

  12. [Results of the morphological examination of stomach in the different terms after stitching of a perforation duodenal ulcer].

    Science.gov (United States)

    Tumanidze, G Z; Khardzeishvili, O M; Chkhikvadze, T F; Khazaradze, D V

    2005-01-01

    Analyses of 184 cases of morphological examination of resected part of stomach in the different terms after stitching of a perforative duodenal ulcer is presented. The authors confirm, that ulcerative disease of duodenum and it's various complications including perforation is associated with antrum gastritis in all cases, which has superficial character. The authors are confirming that a gastric metaplasia of a mucosa of duodenum assists to the helicobacterial colonization, which represents one of the main factors of an ulcer formation along with a fast transition of acid gastric contents into the duodenum. The authors consider the gastric metaplasia as preulcerative pathological condition of duodenum.

  13. 40 CFR 799.9135 - TSCA acute inhalation toxicity with histopathology.

    Science.gov (United States)

    2010-07-01

    .... The bronchoalveolar lavage is designed to be a rapid screening test to provide an early indicator of...; stomach; duodenum; jejunum; ileum; cecum; colon; rectum; urinary bladder; representative lymph...

  14. Secretion of goblet cell serine proteinase, ingobsin, is stimulated by vasoactive intestinal polypeptide and acetylcholine

    DEFF Research Database (Denmark)

    Poulsen, Steen Seier; Nexø, Ebba

    1987-01-01

    Ingobsin is localized to the intestinal goblet cells in the rat and in man. In the present study, we investigated the effect of vasoactive intestinal polypeptide (VIP) and acetylcholine on the secretion of ingobsin from the proximal duodenum. Intravenous infusion of VIP or acetylcholine increased...... the concentration of ingobsin in duodenal secretion, while the concentration in the duodenum was unchanged. Simultaneous infusion of VIP and acetylcholine increased the concentration of ingobsin in duodenal secretion and decreased the concentration of ingobsin in the duodenum. This study demonstrates that secretion...... of ingobsin from the proximal duodenum is exocrine and can be stimulated by VIP and acetylcholine....

  15. Repeated-Dose and Reproductive/Developmental Toxicity of NTO (3-Nitro-1,2,4-Triazol-5-One) in the Rat

    Science.gov (United States)

    2014-03-21

    than 5000 milligrams per kilogram (mg/kg) . In addition, NTO was reported to be a mild skin and eye irritant but was not a dermal sensitizer (Los...pancreas; testes; uterus; aorta; esophagus; stomach; duodenum; jejunum; ileum; caecum; colon ; rectum; urinary bladder; representative lymph node...adrenals; pancreas; testes; uterus; aorta; esophagus; stomach; duodenum; jejunum; ileum; cecum; colon ; rectum; urinary bladder; representative lymph

  16. Pancreas-preserving total duodenectomy: a 10-year experience

    DEFF Research Database (Denmark)

    Penninga, Luit; Svendsen, Lars Bo

    2011-01-01

    Traditionally, severe pathology of the duodenum has been treated by a pancreaticoduodenectomy using Whipple's operation. Pancreas-preserving total duodenectomy (PPTD) was introduced in the late 1990s as an alternative to Whipple's operation for selected diseases of the duodenum. We report our 10...

  17. Roux-en-Y stomach surgery for weight loss (image)

    Science.gov (United States)

    ... stomach and attaching it directly to the small intestine, bypassing a large part of the stomach and duodenum. Not only is the stomach pouch too small to hold large amounts of food, but by skipping the duodenum, fat absorption is substantially reduced.

  18. 胰十二指肠切除术与保留十二指肠胰头次全切除术治疗胰头肿块型慢性胰腺炎的临床疗效%Clinical efficacy of pancreaticoduodenectomy and duodenum-preserving pancreatic head resection for the treatment of chronic pancreatitis with mass in the head of the pancreas

    Institute of Scientific and Technical Information of China (English)

    贺舜民; 李志强; 余枭; 余灿; 朱红伟; 汪东文; 宋军

    2015-01-01

    Objective To investigate the clinical efficacy of pancreaticoduodenectomy (PD) and duodenumpreserving pancreatic head resection (DPPHR,including Beger,Frey and Berne procedures)for the treatment of chronic pancreatitis (CP) with mass in the head of the pancreas.Methods The clinical data of 48 patients with CP who were admitted to the Armed Police Corps Hospital of Hunan province(13) and the Third Xiangya Hospital of Central South University (35) between January 2007 and December 2013 were retrospectively analyzed.The operation methods were selected according to clinical symptoms,imaging findings and intraoperative pathological examinations.Twenty-three patients receiving PD (Whipple procedure or pylorus-preserving PD) were allocated into PD group and 25 receiving DPPHR (Beger,Frey and Berne procedures) were allocated into DPPHR group.The operation time,volume of intraoperative blood loss,rate of postoperative pain relief,changes of pancreatic endocrine and exocrine function,complications,duration of hospital stay and hospital expenses in the 2 groups were analyzed.Patients were followed up by telephone interview and outpatient examination up to September 2014.Measurement data with normal distribution were presented as (x) ± s.Comparison between groups was analyzed using the t test.Count data were analyzed using chi-square test or Fisher exact probability.Results Of the 23 patients in the PD group,15 patients received Whipple procedure and 8 patients received pylorus preserving PD.Of 25 patients in the DPPHR group,8 patients received Beger procedure,13 patients received Frey procedure and 4 patients received Berne procedure.The operation time and volume of intraoperative blood loss were (5.5 ± 0.4) hours,(372 ± 174) mL in the PD group,and (4.2 ± 0.6) hours,(272 ± 114) mL in the DPPHR group,showing significant differences between the 2 groups (t =8.712,2.375,P < 0.05).Three patients had massive hemorrhage in the PD group and 2 patients receiving Beger procedure had massive hemorrhage due to portal vein injury,with no significant difference (x2=0.010,P > 0.05).The intraoperative pathologic examinations of frozen section showed chronic inflammation in all pancreatic tissue samples with fibrous tissue proliferations.Overall pain relief rate was 95.7% (22/23) in the PD group,including 20 complete remissions and 2 partial remissions,and overall pain relief rate was 92.0% (23/25) in the PD group,including 18 complete remissions and 5 partial remissions,which were no different in overall pain relief rate (x2 =0.000,P > 0.05).The morbidity of postoperative diabetes mellitus and dyspepsia with fatty diarrhea were 38.9% (7/18) and 35.7% (5/14) in the PD group,which were no different from 9.5% (2/21) and 20.0% (3/15) in the DPPHR group (x2=3.200,0.281,P >0.05).The incidence of postoperative complication was 30.4% (7/23) in the PD group,including 1 case of intra-abdominal hemorrhage,pancreatic fistula and localized peritonitis,1 case of pancreatic fistula,2 cases of biliary fistula,3 cases of delayed gastric emptying.Patients with pancreatic fistula and biliary fistula recovered after 1-week sufficient drainage.The incidence of postoperative complication was 4.0% (1/25) in the DPPHR group,including 1 case of pancreatic fistula,showing significant difference in incidence of postoperative complication (x2=4.274,P < 0.05).The duration of postoperative stay and hospital expense were (12.4 ± 2.5) days and (57 751 ± 6 772) yuan in the PD group,which were significantly different from (8.2 ± 1.8) days and (49 109 ± 6 168)yuan in the DPPHR group (t =6.576,4.645,P < 0.05).Forty-eight patients were followed up with a median time of 51.6 months (9.0-92.0 months).Of the 2 patients died,1 patient who underwent Frey procedure died 3 months after diagnosis of pancreatic cancer due to epigastric pain at postoperative month 6,the other died 2 years later due to cardiovascular disease.Among 48 patients with follow-up,1 received biliary-intestine drainage 6 months later and other patients had no recurrence or canceration.Conclusions DPPHR is safe and effective for chronic pancreatitis with mass in the head of the pancreas,having advantages such as shorter duration of operation,less intraoperative hemorrhage,faster postoperative recover,shorter duration of hospital stay and delayed hypofunction of pancreatic endocrine and exocrine function.But DPPHR cannot completely replace PD,It is necessary to master indications for all kinds of operations and choose proper operative approaches based on lesion characteristics.%目的 探讨胰十二指肠切除术(PD)和保留十二指肠胰头次全切除术(DPPHR,包括Beger术、Frey术、Berne术)治疗胰头肿块型慢性胰腺炎的临床疗效.方法 回顾性分析2007年1月至2013年12月武警湖南省总队医院收治的13例和中南大学湘雅三医院收治的35例慢性胰腺炎患者的临床资料.根据患者临床症状、影像学表现和术中病理学检查结果选择手术方式.其中23例行PD(经典Whipple术或保留幽门的PD)患者为PD组,25例行DPPHR术(Beger术、Frey术或Berne术)患者为DPPHR组.分析两组患者的手术时间、术中出血量、术后疼痛缓解率、胰腺内外分泌功能改变、手术并发症、术后住院时间、住院费用等指标.通过电话和门诊复查等方式随访,随访时间截至2014年9月.正态分布的计量资料以x±s表示,两组比较采用t检验;计数资料采用x2检验及其校正公式或Fisher确切概率法.结果 PD组23例患者中,行Whipple术15例,行保留幽门PD 8例;DPPHR组25例患者中,行Beger术8例,Frey术13例,Berne术4例.PD组和DPPHR组患者的手术时间、术中出血量分别为(5.5±0.4)h和(4.2±0.6)h,(372±174) mL和(272±114) mL,两组比较,差异均有统计学意义(t=8.712,2.375,P<0.05).PD组有3例患者术中出现大出血,DPPHR组2例行Beger术患者术中损伤门静脉出现大出血;两组比较,差异无统计学意义(x2=0.010,P>0.05).两组患者术中快速冷冻切片病理学检查结果均为胰腺慢性炎症改变,伴纤维组织增生.PD组患者术后疼痛总缓解率为95.7%(22/23),其中疼痛完全缓解20例,疼痛缓解2例;DPPHR组患者术后疼痛总缓解率为92.0% (23/25),其中疼痛完全缓解18例,疼痛缓解5例;两组患者术后疼痛总缓解率比较,差异无统计学意义(x2=0.000,P>0.05).PD组患者术后糖尿病发病率为38.9%(7/18),消化不良和脂肪泻发生率为35.7%(5/14);DPPHR组分别为9.5% (2/21)和20.0% (3/15),两组患者比较,差异均无统计学意义(x2=3.200,0.281,P>0.05).PD组患者术后并发症发生率为30.4%(7/23),其中合并腹腔出血、胰液漏及局限性腹膜炎患者1例,胰液漏1例,胆瘘2例,胃排空延迟3例.胰液漏和胆瘘患者经充分引流约1周后痊愈.DPPHR组患者术后1例患者发生胰液漏,并发症发生率为4.0% (1/25).两组患者术后并发症发生率比较,差异有统计学意义(x2=4.274,P<0.05).PD组患者术后住院时间和住院费用分别为(12.4±2.5)d和(57 751±6772)元,DPPHR组分别为(8.2±1.8)d和(49 109±6 168)元,两组比较,差异均有统计学意义(t=6.576,4.645,P<0.05).48例患者获得随访,平均随访时间为51.6个月(9.0 ~92.0个月).随访期间2例患者死亡,1例行Frey术,术后半年再次出现腹上区疼痛并确诊为胰腺癌,确诊后3个月死亡;1例行PD,术后2年因心血管系统疾病死亡.随访患者中1例半年后再行胆肠引流术,其余患者随访期间未出现复发和癌变.结论 DPPHR用于治疗胰头肿块型慢性胰腺炎安全有效,具有手术时间短、术中出血量少、术后恢复快、住院时间短并延迟胰腺内外分泌功能减退等优势.但DPPHR不能完全替代PD,因此,需把握各类手术的适应证,结合病变特点选择合适的手术方式.

  19. Reflexogenic relaxation gastroduodenography by the acupuncture method

    Energy Technology Data Exchange (ETDEWEB)

    Rabkin, I.Kh.; Tsibulyak, V.N.; Mnatsakyan, K.A.; Kondorskaya, I.L.; Galkina, T.V.

    The communication is based upon the results of x-ray examination of the stomach and duodenum in 63 patients with stenoses of the pyloroduodenal zone, cicatrical deformities of the duodenal bulb, bulbar ulcer, duodenal organic lesions, and functional stenosis of the loop. First a routine X-ray examination of the stomach and duodenum was performed using barium-water mixture, then followed acupuncture aimed at hypotension in the definite points of the floor of the auricle where branches of the vagus innervating the stomach and duodenum are located. As distinct from pharmacological relaxation this method produces a purpose-oriented selective effect.

  20. Novel deployment of a covered duodenal stent in open surgery to facilitate closure of a malignant duodenal perforation

    Directory of Open Access Journals (Sweden)

    Psaila Josephine

    2009-10-01

    Full Text Available Abstract Background Its a dilemma to attempt a palliative procedure to debulk the tumour and/or prevent future obstructive complications in a locally advanced intra abdominal malignancy. Case presentation A 38 year old Vietnamese man presented with a carcinoma of the colon which had invaded the gallbladder and duodenum with a sealed perforation of the second part of the duodenum. Following surgical exploration, it was evident that primary closure of the perforated duodenum was not possible due to the presence of unresectable residual tumour. Conclusion We describe a novel technique using a covered duodenal stent deployed at open surgery to aid closure of a malignant duodenal perforation.

  1. Gastrointestinal Allergy in the Practice of Pediatrician and Pediatric Allergist

    Directory of Open Access Journals (Sweden)

    Ye.N. Okhotnikova

    2015-08-01

    Full Text Available This article describes a case of the gastrointestinal food allergy (allergic gastroduodenitis, which was confirmed by biopsy of the mucosa of esophagus, stomach and duodenum, in a child with atopic dermatitis.

  2. Personal Experience in TCM Treatment of Reflux Esophagitis

    Institute of Scientific and Technical Information of China (English)

    洪流

    2003-01-01

    @@ Reflux esophagitis falls into the category of reflux gastroesophagopathy. It is characterized by inflammation, erosion, ulcer formation and fibrosis of the esophageal mucous membrane due to reflux of the content of the stomach and duodenum into the esophagus.

  3. 40 CFR 799.1560 - Diethylene glycol butyl ether and diethylene glycol butyl ether acetate.

    Science.gov (United States)

    2010-07-01

    ... bladder (if present); esophagus; stomach; duodenum; jejunum; ileum; cecum; colon; rectum; urinary bladder... and eosin screening does not provide such information, a battery of stains shall be used to assess...

  4. Functional changes after pancreatoduodenectomy: Diagnosis and treatment

    NARCIS (Netherlands)

    T.C. Tran; J.J.B. van Lanschot (Jan); M.J. Bruno (Marco); C.H.J. van Eijck (Casper)

    2009-01-01

    textabstractRelatively little is known about the gastrointestinal function after recovery of a pancreatoduodenectomy. This review focuses on the functional changes of the stomach, duodenum and pancreas that occur after pancreatoduodenectomy. Although the mortality in relation to

  5. Peptic Ulcer

    Science.gov (United States)

    A peptic ulcer is a sore in the lining of your stomach or your duodenum, the first part of your small ... and goes for several days or weeks Peptic ulcers happen when the acids that help you digest ...

  6. Effects of energy and protein restriction, followed by nutritional recovery on morphological development of the gastrointestinal tract of weaned kids

    National Research Council Canada - National Science Library

    Sun, Z H; He, Z X; Zhang, Q L; Tan, Z L; Han, X F; Tang, S X; Zhou, C S; Wang, M; Yan, Q X

    2013-01-01

    ...), nutritional restriction period (7 to 48 d), and recovery period (49 to 111 d). Three kids from each group were killed at d 48 and 111, and the rumen, duodenum, jejunum, and ileum were harvested. On d 48...

  7. Upper GI Bleeding in Children

    Science.gov (United States)

    Upper GI Bleeding in Children What is upper GI Bleeding? Irritation and ulcers of the lining of the esophagus, stomach or duodenum can result in upper GI bleeding. When this occurs the child may vomit ...

  8. Annular pancreas

    Science.gov (United States)

    ... chap 330. Semrin MG, Russo MA. Anatomy, histology, embryology, and developmental anomalies of the stomach and duodenum. ... M. is also a founding member of Hi-Ethics and subscribes to the principles of the Health ...

  9. Some aspects of the morphology, population structure and larval ...

    African Journals Online (AJOL)

    1987-06-26

    Jun 26, 1987 ... parasite has a high prevalence of infection (68,9%) in the X laevis population studied and shows a ... (c) intestine: from the posterior end of the duodenum to ..... hosts of C. namaquensis are aquatic, amphibians which.

  10. the roles of gastric acid and nitric oxide

    African Journals Online (AJOL)

    Dr Olaleye

    well as mucosal blood flow via increased NO mechanism. INTRODUCTION1 ... transection was made at the junction of the pylorus with the duodenum where a pyloric ..... Fujishita, T., Furutani, K and Okabe, S. (2003):. Pharmacological control ...

  11. Upper GI Bleeding in Children

    Science.gov (United States)

    Upper GI Bleeding in Children What is upper GI Bleeding? Irritation and ulcers of the lining of the esophagus, stomach or duodenum can result in upper GI bleeding. When this occurs the child may vomit blood ...

  12. Oral vaccination with attenuated Salmonella choleraesuis C500 ...

    African Journals Online (AJOL)

    user

    Several H. pylori virulence factors have been identified, .... twice in sterile 0.9% NaCl, and the pylorus, liver, lung, duodenum and spleen from mice .... duodenal ulcer risk. Cancer Res. ... developing adenocarcinoma of stomach. Cancer Res.

  13. Drooling

    Science.gov (United States)

    ... Hess JM, Lowell MJ. Esophagus, stomach, duodenum. In: Marx JA, Hockberger RS, Walls RM, et al, eds. ... FR, Berge LR. Upper respiratory tract infections. In: Marx JA, Hockberger RS, Walls RM, et al, eds. ...

  14. Large pedinculated antral hyperplastic gastric polyp traversed the bulbus causing outlet obstruction and iron deficiency anemia:endoscopic removal

    Institute of Scientific and Technical Information of China (English)

    Murat Alper; Yusuf Akcan; Olcay Belenli

    2003-01-01

    We present here a large (3 cm) hyperplastic gastric polyp prolapsed into duodenum and caused outlet obstruction and iron deficiency anemia in 60 years old male patient.Endoscopic removal was performed successfully.

  15. Duodenal perforation in childhood dermatomyositis

    Energy Technology Data Exchange (ETDEWEB)

    Magill, H.L.; Hixson, S.D.; Whitington, G.; Igarashi, M.; Hannissian, A.

    1984-01-01

    Perforation of the duodenum is an uncommon, but serious complication which may occur in children with dermatomyositis. In this disease vasculitis may involve the bowel to a variable extent and result in radiologic manifestations of intestinal injury ranging from benign pneumatosis intestinalis to signs of bowel perforation. We report two children with dermatomyositis in whom perforation of the second portion of the duodenum occurred. This serious complication should be considered in any child with dermatomyositis when extraluminal gas is suspected on abdominal radiographs.

  16. [Cycloferon in treating duodenal ulcers in rats].

    Science.gov (United States)

    Bul'on, V V; Khnychenko, L K; Sapronov, N S; Kuznetsova, N N; Anikin, V B; arinenko, R Iu; Kovalenko, A L; Alekseeva, L E

    2001-01-01

    The possibility of using cycloferon (interferon inductor) for a complex treatment (in combination with the main drug solcoseryl possessing pronounced therapeutic properties) of duodenum ulcers was experimentally studied in male rats. The experiments showed a considerable difference in the interferon status of animals with model duodenum ulcers treated with cycloferon, solcoseryl, their combination, and placebo (control). The healing effect of solcoseryl administered in combination with cycloferon exceeded that of each component administered separately.

  17. Superior mesenteric artery compression syndrome - case report

    OpenAIRE

    Paulo Rocha França Neto; Rodrigo de Almeida Paiva; Antônio Lacerda Filho; Fábio Lopes de Queiroz; Teon Noronha

    2011-01-01

    Superior mesenteric artery syndrome is an entity generally caused by the loss of the intervening mesenteric fat pad, resulting in compression of the third portion of the duodenum by the superior mesenteric artery. This article reports the case of a patient with irremovable metastatic adenocarcinoma in the sigmoid colon, that evolved with intense vomiting. Intestinal transit was carried out, which showed important gastric dilation extended until the third portion of the duodenum, compatible wi...

  18. Adenoma viloso com transformação carcinomatosa da ampola de Vater Villous adenoma with carcinomatous degeneration of the ampulla of Vater

    Directory of Open Access Journals (Sweden)

    Guilherme Pinto Bravo Neto

    2001-02-01

    Full Text Available Villous adenomas of the duodenum and ampulla of Vater are uncommon, but they have been diagnosed more frequently with the increasing use of upper gastrointestinal endoscopy. Differential diagnosis with villous adenocarcinoma may be difficult. The authors present a case of a 47-year-old man with a giant villous adenoma of the duodenum, with intermittent jaundice, that was treated by pancreatoduodenectomy.

  19. [Persistent duodenal septum in an adult].

    Science.gov (United States)

    Helwing, E; Echtermeyer, V; Otten, G

    1977-02-01

    A case of duodenal obstruction by a congenital duodenal web in a 34-year-old woman is presented. A mucosal diaphragm obstructed the duodenum. It showed an excentric opening of 0.8 cm diameter, but the dilated diaphragm caused a total stop during the last months. Despite a typical history, exact X-ray, and endoscopic examination, the correct preoperative diagnosis was not found, because nobody thought it possible, that a mucosal diapharm of the duodenum could persist for 34 years.

  20. Adenoma viloso com transformação carcinomatosa da ampola de Vater

    Directory of Open Access Journals (Sweden)

    Guilherme Pinto Bravo Neto

    Full Text Available Villous adenomas of the duodenum and ampulla of Vater are uncommon, but they have been diagnosed more frequently with the increasing use of upper gastrointestinal endoscopy. Differential diagnosis with villous adenocarcinoma may be difficult. The authors present a case of a 47-year-old man with a giant villous adenoma of the duodenum, with intermittent jaundice, that was treated by pancreatoduodenectomy.

  1. Tryptophan rich diet as a new approach to study the serotoninergic enteropancreatic axis.

    OpenAIRE

    Goke, B.; Richter, G; Keim, V; Arnold, R

    1987-01-01

    The influence of a tryptophan enriched diet (L-tryptophan added as 1% of total diet), fed over 10 days, on the rat duodenum and pancreas was studied by immunohistology, measurements of serotonin and tryptophan tissue concentrations by HPLC, and incubations of pancreatic lobules. Ingestion of a tryptophan enriched diet resulted in increased contents of tryptophan and serotonin in the duodenum that was not accompanied by a significant change of the serotonin cell density. Neither basal nor CCK-...

  2. Stimulatory effect of Coca-Cola on gastroduodenal HCO3- secretion in rats.

    Science.gov (United States)

    Sasaki, Y; Aihara, E; Ise, F; Kita, K; Takeuchi, K

    2007-10-01

    We examined the effect of various carbonated beverages, especially Coca-Cola, on the HCO3- secretion in the rat stomach and duodenum. Under urethane anaesthesia, a chambered stomach or a proximal duodenal loop was perfused with saline, and HCO3- secretion was measured at pH 7.0 using a pH-stat method and by adding 2 mM HCl. The amount of CO2 contained in these beverages was about 4-7 g/mL. Coca-Cola topically applied to the mucosa for 10 min significantly increased the HCO3- secretion in both the stomach and the duodenum. The HCO3- response in the duodenum was totally abolished by indomethacin and also partially inhibited by acetazolamide, an inhibitor of carbonic anhydrase. Likewise, the response in the stomach was also markedly inhibited by either acetazolamide or indomethacin. The mucosal application of Coca-Cola increased the PGE2 contents in both the stomach and the duodenum. Other carbonated beverages, such as sparkling water, Fanta Grape or cider, also increased the HCO3- secretion in these tissues. These results suggest that Coca-Cola induces HCO3- secretion in both the stomach and the duodenum, and these responses may be attributable to both the intracellular supply of HCO3- generated via carbonic anhydrase, and endogenous PGs, probably related to the acidic pH of the solution.

  3. Regulation of gastroduodenal motility: acyl ghrelin, des-acyl ghrelin and obestatin and hypothalamic peptides.

    Science.gov (United States)

    Fujimiya, Mineko; Ataka, Koji; Asakawa, Akihiro; Chen, Chih-Yen; Kato, Ikuo; Inui, Akio

    2012-01-01

    Real-time measurements for gut motility in conscious rats or mice combined with intracerebroventricular or intravenous injection of peptide agonists or antagonists allow us to understand the regulatory mechanism of gastrointestinal motility. Neuropeptide Y (NPY) in the arcuate nucleus in the hypothalamus stimulates the fasted motility in the duodenum, while urocortin in the paraventricular nucleus inhibits fed and fasted motility in the antrum and duodenum. Acyl ghrelin exerts stimulatory effects on the motility of the antrum and duodenum in both the fed and fasted state of animals. NPY Y2 and Y4 receptors in the brain may mediate the action of acyl ghrelin, and vagal afferent pathways might be involved in this mechanism. Des-acyl ghrelin exerts inhibitory effects on the motility of the antrum but not on the motility of the duodenum in the fasted state of animals. CRF type 2 receptor in the brain may mediate the action of des-acyl ghrelin, and vagal afferent pathways might not be involved in this mechanism. Obestatin exerts inhibitory effects on the motility of the antrum and duodenum in the fed state but not in the fasted state of animals. CRF type 1 and type 2 receptors in the brain may mediate the action of obestatin, and vagal afferent pathways might be partially involved in this mechanism.

  4. Duodenal Bleeding from Metastatic Renal Cell Carcinoma

    Science.gov (United States)

    Rustagi, Tarun; Rangasamy, Priya; Versland, Mark

    2011-01-01

    Massive upper gastrointestinal bleeding due to malignancy is relatively uncommon and the duodenum is the least frequently involved site. Duodenal metastasis is rare in renal cell carcinoma (RCC) and early detection, especially in case of a solitary mass, helps in planning further therapy. We report a case of intractable upper gastrointestinal bleeding from metastatic RCC to the duodenum. The patient presented with melena and anemia, 13 years after nephrectomy for RCC. On esophagogastroduodenoscopy, a submucosal mass was noted in the duodenum, biopsies of which revealed metastatic RCC. In conclusion, metastasis from RCC should be considered in nephrectomized patients presenting with gastrointestinal symptoms and a complete evaluation, especially endoscopic examination followed by biopsy, is suggested. PMID:21577373

  5. Duodenal Bleeding from Metastatic Renal Cell Carcinoma

    Directory of Open Access Journals (Sweden)

    Tarun Rustagi

    2011-04-01

    Full Text Available Massive upper gastrointestinal bleeding due to malignancy is relatively uncommon and the duodenum is the least frequently involved site. Duodenal metastasis is rare in renal cell carcinoma (RCC and early detection, especially in case of a solitary mass, helps in planning further therapy. We report a case of intractable upper gastrointestinal bleeding from metastatic RCC to the duodenum. The patient presented with melena and anemia, 13 years after nephrectomy for RCC. On esophagogastroduodenoscopy, a submucosal mass was noted in the duodenum, biopsies of which revealed metastatic RCC. In conclusion, metastasis from RCC should be considered in nephrectomized patients presenting with gastrointestinal symptoms and a complete evaluation, especially endoscopic examination followed by biopsy, is suggested.

  6. Stereomicroscopic and ultrastructural characterization of propionitrile-induced duodenal ulcer in the rat

    DEFF Research Database (Denmark)

    Poulsen, Steen Seier

    1978-01-01

    Acute duodenal ulcer produced by subcutaneous injection of propionitrile in rats was studied by stereo, light, and electron microscopy in order to gain insight into the localization and mechanism of initial cell injury. Stereomicroscopy revealed an initial fissuring and splitting of the tips...... of the villus folds within 4 hours after two injections of propionitrile. This was followed by sloughing of the epithelium, shortening and effacement of the villus folds, and within 24 hours the appearance of discrete ulcers in the mucosa of the proximal duodenum. In most of the rats, two ulcers developed......: the first and larger ulcer was on the antimesenteric side of the duodenum, and the other, a small and more superficial one, was on the opposite wall. Ultrastructural lesions appeared in the absorptive epithelial cells of the proximal duodenum within 5 hours following a single dose of propionitrile...

  7. Duodenal bleeding from metastatic renal cell carcinoma.

    Science.gov (United States)

    Rustagi, Tarun; Rangasamy, Priya; Versland, Mark

    2011-04-20

    Massive upper gastrointestinal bleeding due to malignancy is relatively uncommon and the duodenum is the least frequently involved site. Duodenal metastasis is rare in renal cell carcinoma (RCC) and early detection, especially in case of a solitary mass, helps in planning further therapy. We report a case of intractable upper gastrointestinal bleeding from metastatic RCC to the duodenum. The patient presented with melena and anemia, 13 years after nephrectomy for RCC. On esophagogastroduodenoscopy, a submucosal mass was noted in the duodenum, biopsies of which revealed metastatic RCC. In conclusion, metastasis from RCC should be considered in nephrectomized patients presenting with gastrointestinal symptoms and a complete evaluation, especially endoscopic examination followed by biopsy, is suggested.

  8. Gastric and Duodenal Pseudomelanosis: An Extended Unusual Finding in a Patient with End Stage Kidney Disease

    Directory of Open Access Journals (Sweden)

    Noor Ul Ain Qureshi

    2016-01-01

    Full Text Available Gastric and duodenal pseudomelanosis is a rare endoscopic mucosal finding, characterized by the accumulation of iron in macrophages of the lamina propria of the stomach and duodenum. The clinical significance and long term sequelae have not been clarified yet. However, this benign condition is associated with a variety of clinical conditions, such as essential hypertension, chronic renal failure, diabetes mellitus, long term intake of iron supplements, and furosemide. Duodenal pseudomelanosis appears to be more common than gastric pseudomelanosis given the fact that a few cases of gastric pseudomelanosis have been reported in the literature so far. We report a case of 88-year-old lady with ESRD who is maintained on hemodialysis and presented with abdominal pain. An upper GI endoscopy showed discoloration of the antrum of the stomach and most portion of her duodenum. Histopathology report confirmed the presence of iron laden macrophages in the lamina propria of both stomach and duodenum.

  9. Gastric and Duodenal Pseudomelanosis: An Extended Unusual Finding in a Patient with End Stage Kidney Disease

    Science.gov (United States)

    Qureshi, Noor Ul Ain; Younus, Muhammad Faraz; Alavi, Kourosh

    2016-01-01

    Gastric and duodenal pseudomelanosis is a rare endoscopic mucosal finding, characterized by the accumulation of iron in macrophages of the lamina propria of the stomach and duodenum. The clinical significance and long term sequelae have not been clarified yet. However, this benign condition is associated with a variety of clinical conditions, such as essential hypertension, chronic renal failure, diabetes mellitus, long term intake of iron supplements, and furosemide. Duodenal pseudomelanosis appears to be more common than gastric pseudomelanosis given the fact that a few cases of gastric pseudomelanosis have been reported in the literature so far. We report a case of 88-year-old lady with ESRD who is maintained on hemodialysis and presented with abdominal pain. An upper GI endoscopy showed discoloration of the antrum of the stomach and most portion of her duodenum. Histopathology report confirmed the presence of iron laden macrophages in the lamina propria of both stomach and duodenum. PMID:27042366

  10. Gastrointestinal hormones and motility of the human sphincter of Oddi.

    Science.gov (United States)

    Ono, K; Suzuki, H; Hada, R; Sasaki, M; Endoh, M

    1985-07-01

    We investigated the mechanism of humoral control of bile discharge into the duodenum. The actions of the GB, SO and duodenum were monitered by cinecholecystocholangiogrphy combined with manometry of the SO area using a hydraulic-capillary infusion system or MIKRO-TIP, and these were correlated with the plasma concentrations of GI hormones. We concluded that one of the most significant roles of the sphincter of Oddi is to limit bile flow. This postulation was favored by the observation of so-called 'spasm' of the SO where 8 to 10 contractions per min., in contrast to 'normal' contraction of 2 to 4 per min., were seen. On this special occasion, no discharge of the contrast material into the duodenum was noticed. Exogenous or endogenous CCK causes a coordinated action of the GB contraction, the SO relaxation and relaxation of the adjacent segment of the duodenum, resulting in an effective discharge of bile into the duodenum. The effect of Pancreozymin on bile discharge revealed by endoscopic cinematography was that Pancreozymin first made the orifice of the papilla of Vater widely open with a profuse bile flow and following this, caused a repeated shuttering action of the orifice with minimal discharge of bile. This observation opposes the opinion that active SO contractile activity is necessary to bile flow into the duodenum. Caerulein or CCK-33 caused no 'post-inhibition' enhancement of the SO activity that was seen in case of Pancreozymin administration. Motilin, calcium or other substances contained in Pancreozymin (Boots) might be causative for this enhancement.

  11. Neuropeptide Y, peptide YY, and pancreatic polypeptide modulate duodenal and colonic motility at a thoracic spinal site in rats.

    Science.gov (United States)

    Wager-Pagé, S A; Ghazali, B; Anderson, W; Veale, W L; Davison, J S

    1992-01-01

    Neuropeptide Y, PYY, and PP (200 pmol) alter intraluminal pressure in the duodenum and colon of rats following their administration into the thoracic (T8-T10) region of the spinal cord. Neuropeptide Y decreases the tone of the duodenum and the colon following intrathecal (T8-T10) administration prior to an increase in tone to baseline or greater. There is no effect on intraluminal pressure of either the duodenum or the colon following intrathecal administration of NPY or PP into the lumbar (L4-L5) region of the spinal cord. Following intrathecal (T8-T10) administration of PYY and PP, increases in intraduodenal pressures are observed (+2.1 and +3.0 mmHg from saline baseline). Phasic contractions of the duodenum are increased following intrathecal administration of PYY into the thoracic spinal cord of rats. Neuropeptide Y, PYY, and PP increase intracolonic pressure +2.2, +3.3, and +3.7 mmHg from saline baseline, respectively. Phasic contractions of the colon are increased following PP intrathecal thoracic administration. Responsiveness of the duodenum or colon to the different ligands of the PP-fold peptide family in the absence of alpha-adrenergic blockade did not vary. The increases in intraluminal pressure of the duodenum and colon following intrathecal administration of the PP-fold peptides are attenuated by both alpha-1 adrenergic (prazosin) and alpha-2 adrenergic (yohimbine) blockade. There is a difference in responsiveness of the colon between the ligands of the PP-fold family in the presence of the alpha-2 adrenergic blockade. The findings of this study indicate that duodenal and colonic motility are modulated by the PP-fold peptides at thoracic spinal sites via alteration of sympathetic outflow.

  12. Endoscopic treatment of a pediatric case of choledochocele associated with choledocholithiasis

    Directory of Open Access Journals (Sweden)

    Dr. Mehmet Saraç, M.D.

    2017-06-01

    Full Text Available Choledochocele (type 3 choleduct cyst is rarely seen in children. Here we report the case of a 5-year-old female who complained of abdominal pain for 2 years. A choledochocele with a diameter of 31 mm localized on the second part of the duodenum, in the periampullary region, was detected. Upper gastrointestinal endoscopy revealed a mass starting from the second part of the duodenum and constricting the duodenal lumen. A 2-cm-long incision was made at the apex of the cyst using a needle tip sphincterectome and three 5- to 6-mm stones were extracted. There were no postoperative complications.

  13. [Clinico-endoscopic evaluation of the effectiveness of the treatment of uncomplicated gastroduodenal ulcers after local administration of solcoseryl and aevit].

    Science.gov (United States)

    Granov, A M; Aiskhanov, S K; Nikolaev, L I; Smirnova, N A

    1989-12-01

    The complex of conservative measures for noncomplicated peptic ulcers included curative endoscopy in 105 patients, in 61 of them Aevitum was used as an application with additional injections of Solcoseryl around the ulcers. In 44 patients Aevitum was used along with Solcoseryl which was injected into the submucous layer around the pathological focus of the stomach and duodenum. The results obtained gave a convincing evidence of advantages of interstitial administration of Aevitum allowing to considerably shorten time of treatment (up to 10-12 days) of noncomplicated ulcers of the stomach and duodenum.

  14. Slc39a14 Gene Encodes ZIP14, A Metal/Bicarbonate Symporter: Similarities to the ZIP8 Transporter

    OpenAIRE

    2008-01-01

    The mouse and human genomes contain 14 highly conserved SLC39 genes. Viewed from an evolutionary perspective, SLC39A14 and SLC39A8 are the most closely related, each having three noncoding exons 1. However, SLC39A14 has two exons 4, giving rise to Zrt- and Irt-related protein (ZIP)ZIP14A and ZIP14B alternatively spliced products. C57BL/6J mouse ZIP14A expression is highest in liver, duodenum, kidney, and testis; ZIP14B expression is highest in liver, duodenum, brain, and testis; and ZIP8 is h...

  15. Pancreaticoduodenectomy for pancreas carcinoma occurring in the annular pancreas: report of a case.

    Science.gov (United States)

    Kawaida, Hiromichi; Kono, Hiroshi; Watanabe, Mitsuaki; Maki, Akira; Amemiya, Hidetake; Matsuda, Masanori; Fujii, Hideki; Fukasawa, Mitsuharu; Takahashi, Ei; Sano, Katsuhiro; Inoue, Tomohiro

    2015-08-01

    The annular pancreas is a rare congenital anomaly in which a ring of the pancreas parenchyma surrounds the second part of the duodenum. Malignant tumors are extremely rare in patients with an annular pancreas. A 64-year-old man presented with appetite loss and vomiting. Abdominal contrast-enhanced computed tomography (CT) indicated pancreas parenchyma surrounding the second part of the duodenum, and a hypovascular area occupying lesion in the annular pancreas. Subtotal stomach-preserving pancreaticoduodenectomy was performed. Histopathology showed pancreatic carcinoma occurring in the complete annular pancreas.

  16. Congenital preduodenal portal vein

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Sang Jin; Hwang, Mi Soo; Huh, Young Soo; Park, Bok Hwan [College of Medicine, Youngnam University, Gyeongsan (Korea, Republic of)

    1991-03-15

    Congenital preduodenal portal vein, first reported by Knight in 1921, is an extremely rare congenital anomaly in which the portal vein passes anteriorly to the duodenum rather than posteriorly in its normal location. It is of surgical significance because it may cause difficulties in operations involving the gall bladder, biliary duct, or duodenum. Recently, we experienced 2 cases of preduodenal portal vein. One was found during surgical exploration for the diagnosis and correction of malrotation of the bowels and the other in a 3 day-old male newborn associated with dextrocardia, situs inversus, and duodenal obstruction by diaphragm. We report these 2 cases with a review of the literature.

  17. Lactase-phlorizin hydrolase and aminopeptidase N are differentially regulated in the small intestine of the pig

    DEFF Research Database (Denmark)

    Torp, Niels; Rossi, M; Troelsen, J T

    1993-01-01

    moderately from the duodenum to the terminal ileum, the amount of lactase-phlorizin hydrolase mRNA exhibited a sharp maximum in the proximal jejunum. For both enzymes, the level of protein synthesis, studied in cultured mucosal explants, correlated well with the level of mRNA, and no major variation in post......-translational processing or intracellular transport was observed along the intestine. The mRNA/specific-activity ratio for both enzymes was markedly (3-5-fold) higher in the duodenum and proximal jejunum, compared with the ileum. This indicates an increased proximal turnover rate, most likely caused by the presence...

  18. A RARE CASE OF OESOPHAGODUODENAL VARICES

    Directory of Open Access Journals (Sweden)

    Keisham

    2015-10-01

    Full Text Available Varices are sequelae of portal hypertension and can occur in both cirrhotic and noncirrhotic portal hypertension. They are commonly seen in the oesophagus and stomach. Presentation of varix in the duodenum is rare. The commonest site is in the duodenal bulb followed by the second and third parts of duodenum. The treatment of duodenal varices is challenging and various modalities of treatment are described in literature. Here, we present a case of oesophago-duodenal varices successfully treated by endoscopic variceal ligation for oesophageal varix and injection sclerotherapy for duodenal varix.

  19. 2010 SEPTEMBER EDITION FINAL.cdr

    African Journals Online (AJOL)

    1,2 shaped stomach and ends in the duodenum . Equipment. The flexible endoscope is a ... types. The original pure fibreoptic instrument has a flexible bundle of glass fibres that collect the lighted .... 1,2 specimen, and removal of a polyp or tumour . Side effects and risks .... amongst Nigerians with Type 2 Diabetes. Mellitus ...

  20. Distribution of endocrine cells in the digestive tract of Alligator sinensis during the active and hibernating period.

    Science.gov (United States)

    Wang, Huan; Zhang, Shengzhou; Zhou, Naizhen; Wang, Chaolin; Wu, Xiaobing

    2014-10-01

    The digestive tract is the largest endocrine organ in the body; the distribution pattern of endocrine cells varies with different pathological and physiological states. The aim of the present study was to investigate the distributed density of 5-hydroxytryptamine (5-HT), gastrin (GAS), somatostatin (SS) and vasoactive intestinal peptide (VIP) immunoreactive (IR) cells in the digestive tract of Alligator sinensis during the active and hibernating period by immunohistochemical (IHC) method. The results indicated that 5-HT-IR cells were distributed throughout the entire digestive tract, which were most predominant in duodenum and jejunum. The density increased significantly in stomach and duodenum during hibernation. GAS-IR cells were limited in small stomach and small intestine. The density decreased significantly in small stomach during hibernation, while increased in duodenum. What's more, most of the endocrine cells in duodenum were generally spindle shaped with long cytoplasmic processes ending in the lumen during hibernation. SS-IR cells were limited in stomach and small stomach. The density increased in stomach while decreased in small stomach during hibernation, meanwhile, fewer IR cells occurred in small intestine. VIP-IR cells occurred in stomach and small stomach. The density decreased in small stomach, while increased in stomach during hibernation. These results indicated that the endocrine cells in different parts of digestive tract varied differently during hibernation, their changes were adaptive response to the hibernation.

  1. DIETARY FISH OIL-INDUCED CHANGES IN INTRAHEPATIC CHOLESTEROL TRANSPORT AND BILE-ACID SYNTHESIS IN RATS

    NARCIS (Netherlands)

    SMIT, MJ; TEMMERMAN, AM; WOLTERS, H; KUIPERS, F; BEYNEN, AC; VONK, RJ

    Hepatic cholesterol metabolism was studied in rats fed purified diets supplemented (9% wt/wt) with either fish oil (FO) (n-3 fatty acids) or corn oil (CO) (n-6 fatty acids) for 4 wk. Rats were equipped with permanent catheters in heart, bile duct, and duodenum to allow studies under normal feeding

  2. Placement of nasoenteral feeding tubes using magnetic guidance: retesting a new technique.

    Science.gov (United States)

    Ozdemir, B; Frost, M; Hayes, J; Sullivan, D H

    2000-08-01

    To study a new technique of intubating the small bowel using a newly developed nasoenteral feeding tube fitted with a magnet in its tip and guided for placement with an external magnet. The study was performed in medical and surgical wards of a university-affiliated Department of Veterans Affairs hospital on 42 patients referred by their attending physicians for tube placement. The newly designed feeding tube was inserted per nares into the stomach using traditional technique. As the tube was advanced, movement of the hand-held steering magnet was designed to guide the tip of the magnetic nasoenteral tube along the lesser curvature of the stomach, through the pyloric sphincter, and into the duodenum. Portable abdominal radiography confirmed the anatomic location of the tube tip. Fifty-one intubations were performed on 42 subjects. In 45 intubations (88%), tubes passed into the duodenum. Twenty-seven (53%) met criteria for optimal placement in the second portion of the duodenum or distally. Six of 11 tubes (55%) that were not optimally placed were advanced to the distal duodenum on repositioning. Median procedure time for the initial intubations was 30 minutes (interquartile range 15-40). Median procedure time for last 10 intubations improved to 13 minutes (interquartile range 5-20). No complications were related to the procedure. Enteral feeding tube placement using external magnetic guidance is a promising, novel technique which is deserving of further study.

  3. Assessment of the mobile bag method for estimation of in vivo starch digestibility

    DEFF Research Database (Denmark)

    Ghoorchi, Taghi; Lund, Peter; Larsen, Mogens

    2013-01-01

    with starch from barley, wheat, oats, ear maize and maize. Transit times of the mobile bags from duodenum to ileum were not significantly different between feeds. A weak positive correlation was found between in vivo small intestinal and total tract digestibility of starch and disappearance obtained using...

  4. Large Brunner's gland adenoma: Case report and literature review

    Institute of Scientific and Technical Information of China (English)

    Alba Rocco; Pasquale Borriello; Debora Compare; Patrizia De Colibus; Loredana Pica; Alessandro Iacono; Gerardo Nardone

    2006-01-01

    Brunner's gland adenoma (BGA) is a very rare benign tumour of the duodenum, which is usually asymptomatic and discovered incidentally at endoscopy. Occasionally,this lesion may be large, causing upper gastrointestinal haemorrhage or intestinal obstruction. The case had a large Brunner's gland adenoma, presenting melena that was managed by endoscopic excision.

  5. The gut microbiota modulates host amino acid and glutathione metabolism in mice

    DEFF Research Database (Denmark)

    Mardinoglu, Adil; Shoaie, Saeed; Bergentall, Mattias

    2015-01-01

    The gut microbiota has been proposed as an environmental factor that promotes the progression of metabolic diseases. Here, we investigated how the gut microbiota modulates the global metabolic differences in duodenum, jejunum, ileum, colon, liver, and two white adipose tissue depots obtained from...

  6. Solubilisation of poorly water-soluble drugs during in vitro lipolysis of medium- and long-chain triacylglycerols

    DEFF Research Database (Denmark)

    Christensen, Janne Ørskov; Schultz, Kirsten; Mollgaard, Birgitte

    2004-01-01

    The partitioning of poorly soluble drugs into an aqueous micellar phase was exploited using an in vitro lipid digestion model, simulating the events taking place during digestion of acylglycerols in the duodenum. The aqueous micellar phase was isolated after ultracentrifugation of samples obtaine...

  7. Characterisation of a novel proteolytic enzyme localised to goblet cells in rat and man

    DEFF Research Database (Denmark)

    Poulsen, Steen Seier

    1984-01-01

    A proteolytic enzyme, ingobsin , purified from rat duodenal extracts is shown to be localised to intestinal goblet cells of both man and rat. Enzyme positive cells decrease in number from duodenum to colon. The enzyme is a 33 000 Mr protein with an isoelectric point of 5.1. The pH optimum...

  8. Pancreatic bicarbonate secretion involves two proton pumps

    DEFF Research Database (Denmark)

    Novak, Ivana; Wang, Jing; Henriksen, Katrine L.

    2011-01-01

    Pancreas secretes fluid rich in digestive enzymes and bicarbonate. The alkaline secretion is important in buffering of acid chyme entering duodenum and for activation of enzymes. This secretion is formed in pancreatic ducts, and studies to date show that plasma membranes of duct epithelium expres...

  9. 12.DIGESTIVE TRACT

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    12.2.Stomach and duodenum930685 Seroprevalence of Helicobacter pylori insome inhabitants of Beijing district.ZHANG Qiboet al.1st Teach Hosp,Beijing Med Univ,Bei-jing,100034.Beijing Med J 1993;15(4):212-214.With the use of an enzyme-linked immunosorbentassay(ELISA),we estimated 200 healthy blood

  10. Exercise, Intestinal Absorption, and Rehydration

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@ KEYPOINTS 1. The proximal small intestine (duodenum & jejunum) is the primary site of fluid absorption. It absorbs about 50% to 60% of any given fluid load. The colon or large intestine absorbs approximately 80 to 90% of the fluid it receives, but accounts for only about 15% of the total fluid load.

  11. Bouveret syndrome: Primary demonstration of cholecystoduodenal fistula on MR and MRCP study

    Directory of Open Access Journals (Sweden)

    Rajkumar Singh Negi

    2015-01-01

    Full Text Available Bouveret syndrome is an unusual complication of cholelithiasis which results in upper gastrointestinal obstruction due to a gallstone impacted in the duodenum through a bilio-enteric fistula. We present this rare entity which was primarily diagnosed on magnetic resonance (MR and MR cholangiopancreaticography (MRCP study.

  12. Colonic perforations caused by migrated plastic biliary stents

    Energy Technology Data Exchange (ETDEWEB)

    Virgilio, Edoardo; Pascarella, Agauido; Scandavini, Chiara Maria; Frezza, Barbara; Bocchetti, Tommaso; Balducci, Genoveffa [Faculty of Medicine and Psychology ' Sapienza' , St. Andrea Hospital, Rome (Italy)

    2015-04-15

    Endoscopic insertion of plastic endoprostheses has become an integral part of the management of many benign and malignant diseases affecting the hepatobiliary and pancreatic system. Clogging and dislocation into the duodenum are the most frequently described complications following stent placement. Distal migration with or without perforation of the colon is an exceedingly rare phenomenon and the treatment is not well defined, as discussed below.

  13. Immunohistochemical characterization of the lymphocyte and the immunoglobulin-containing cell in the epithelium and the lamina propria of normal human intestines.

    Directory of Open Access Journals (Sweden)

    Matsueda,Kazuhiro

    1991-06-01

    Full Text Available In order to clarify difference of the mucosal immunity in various sites of normal large and small intestines, we studied the population of lymphocyte subsets and immunoglobulin (Ig-containing cells in situ in biopsy specimens taken from various sites (ascending colon, sigmoid colon and rectum of the large intestine and from the duodenum using an immunohistochemical method. Monoclonal antibodies against pan-T (Leu 1, cytotoxic/suppressor T (Leu2a, helper/inducer T (Leu3a, suppressor T (Leu15 and natural killer/K (Leu7 cells, and polyclonal antibodies to human IgG, IgA and IgM were used. In the duodenum, intraepithelial lymphocytes (IELs were more prominent than in the large intestine. Immunoelectron microscopic observation revealed that some Leu2a+ IELs possessed pseudopods extending into intestinal epithelial cells, indicating that some IELs belong to the cytotoxic T cell subset. Leu7+ IELs were scarcely observed and Leu7+/Leu1+ ratio was higher in the large intestine than in the duodenum. Furthermore, the number of Leu7+ cells were more in the distal than the proximal colon. In the lamina propria Ig-containing cells tended to be fewer in the rectum than in the duodenum and the proximal colon. Our findings may suggest the variation of local immune responses and the difference of assigned immunological functions among the various sites of the intestines.

  14. Influence of age, sex and rearing systems on Toll-like receptor 7 (TLR7) expression pattern in gut, lung and lymphoid tissues of indigenous ducks.

    Science.gov (United States)

    Kolluri, Gautham; Ramamurthy, N; Churchil, R R; Dhinakar Raj, G; Kannaki, T R

    2014-02-01

    Abstract 1. The objective of the experiment was to determine the influence of age, sex and rearing system on Toll-like receptor 7 (TLR7) gene expression in gut, lung and lymphoid tissues and physiological responses to stress in male and female indigenous ducks of Tamil Nadu, India. 2. A total of 36 ducks (12 males and 24 females) were obtained from local farmers and tissue samples of gut tissues (duodenum, jejunum, ileum and caecum), lymphoid organs (spleen and bursa) and lungs were collected in RNAlater solution followed by RNA extraction. 3. After normalisation to β-actin (endogenous control) qPCR analysis identified a significant effect of age, sex and rearing system on TLR7 expression in the ducks. 4. A significant up-regulation of TLR7 expression was observed in lungs, duodenum, jejunum, ileum and caecum of sexually mature (45 wk) compared with that of immature ducks (16 wk). Among sexes, male ducks had significantly higher TLR7 expression than female ducks. 5. Age and sex interactions were significant in lungs, duodenum, jejunum and caecum. Ducks reared in an extensive housing system showed significantly higher TLR7 expression in bursa, lungs, duodenum, ileum and caecum compared to intensively reared ducks. There were no effects of age, sex and rearing systems on TLR7 expression in the spleen. 6. The heterophil-to-lymphocyte ratio and serum corticosterone were higher in ducks reared on an intensive system compared with ducks from an extensive rearing system.

  15. Duodenal lipid-induced symptom generation in gastroesophageal reflux disease : role of apolipoprotein A-IV and cholecystokinin

    NARCIS (Netherlands)

    Van Boxel, O. S.; Ter Linde, J. J. M.; Oors, J.; Otto, B.; Feinle-Bisset, C.; Smout, A. J. P. M.; Siersema, P. D.

    2012-01-01

    Background Duodenal lipid intensifies the perception of esophageal acid perfusion. Recently, we showed that genes implicated in lipid absorption were upregulated in the duodenum of fasting gastro-esophageal reflux disease (GERD) patients. This suggests that chylomicron production and secretion may b

  16. Histomorphometric changes of small intestine in pregnant rat.

    Science.gov (United States)

    Sabet Sarvestani, Fatemeh; Rahmanifar, Farhad; Tamadon, Amin

    2015-01-01

    Food intake of rats increases during pregnancy. This requires changes in the structure of the small intestine to absorb additional food. The aim of the present study was to investigate the morphological changes in the layers of small intestine in rats during pregnancy. Duodenum, jejunum and ileum of 18 pregnant Sprague-Dawley rats (day 7, 14 and 21 of pregnancy) were collected. Villous height and width and thickness of lamina propria, tunica muscularis entirely and separately (circular and longitudinal layers) were measured on transverse sections. During pregnancy increasing villi length and muscular layer thickness was observed in duodenum. Furthermore, along with the progress of gestation greatest histomorphometric change in small intestine was observed in the jejunum. The reduction in the ileum histomorphologic indices was observed during pregnancy. In conclusion, increase in histomorphologic indices of duodenum and jejunum supplies more capacity of duodenum to digest food intake during pregnancy and decrease in these indices in ileum controls the absorption of excess produced amino acids and glucose by hyperphagia.

  17. Abomasal secretion and motility in sheep : effect of diet and digesta components

    NARCIS (Netherlands)

    Bruchem, van J.

    1977-01-01

    In sheep fitted with re-entrant cannulas in the proximal duodenum, secretory rates of acid in the abomasum increased with protein or buffering content of the rations.In sheep fitted with an abomasal infusion tube and with duodenal re-entrant cannulas, proteins and inorganic buffers continuously infu

  18. Immunomodulating Lactobacilli in Chicken

    NARCIS (Netherlands)

    M.E. Koenen (Marjorie)

    2004-01-01

    markdownabstract__Abstract__ The gastro-intestinal (GI) tract of a chicken starts with the beak, followed by the esophagus and crop, proventriculus (glandular stomach), gizzard (muscular stomach), duodenum, ileum, a pair of blind elongated caeca, colon and ending in the cloaca. The GI-tract

  19. Endoskopisk ultralydskanning af maligne tumorer i øvre abdomen

    DEFF Research Database (Denmark)

    Mortensen, M B; Hovendal, C P; Pedersen, S A

    1993-01-01

    Endoscopic ultrasonography (EUS) of the upper gastrointestinal tract is a newly developed, non-invasive investigational method. It combines the direct optical picture of the endoscope with a simultaneous ultrasonographic image of the whole wall of the oesophagus, stomach and duodenum as well as o...

  20. Expression of digestive enzymes and nutrient transporters in the small intestine of Eimeria acervulina-infected chickens

    Science.gov (United States)

    Coccidiosis is a major disease of poultry caused by the intestinal protozoa Eimeria. Eimeria acervulina mainly infects the duodenum, causing lesions in epithelial tissue. The objective of this study was to investigate the effect of E. acervulina infection on the expression of 18 nutrient transport...

  1. Duodenal Obstruction as First Presentation of Metastatic Breast Cancer

    Directory of Open Access Journals (Sweden)

    Sami Khairy

    2015-01-01

    Full Text Available The metastatic breast cancer to the duodenum is rare in spite of common breast cancer. In this paper, we are reporting a rare case of 50-year-old lady who presented with intestinal obstruction as result of metastatic breast cancer which completely responds to chemotherapy. The tumor presents again as brain metastasis after stop of Herceptin due to cardiac toxicity.

  2. Catheter perforation of distal oesophagus with duodenal re-entry of catheter. Report of a case

    Energy Technology Data Exchange (ETDEWEB)

    Bundgaard, T.; Kristensen, H.; Lesak, F.

    An unusual case of perforation of the oesophagus is presented. A nasogastric tube had perforated the oesophagus and re-penetrated into the duodenum, and thereby re-entered the gastrointestinal tract without perforating the peritoneum and without causing the classical clinical signs of oesophageal perforation. Treatment was started 31 days after the perforation.

  3. Experiment list: SRX121286 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX121286 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 51178219,77.5,11.0,52224 Chromatin accessibility assay of Fetal Stomach MO...on, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TIS

  4. Experiment list: SRX121291 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX121291 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 51230055,74.0,12.3,59359 Chromatin accessibility assay of Fetal Stomach MO...on, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TIS

  5. Experiment list: SRX055192 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX055192 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 27551958,91.6,7.4,30450 Chromatin accessibility assay of Fetal Stomach MOL...n, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISS

  6. Experiment list: SRX214043 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX214043 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 595242582,80.0,40.4,244393 Digital Genomic Footprinting assay of Fetal Stomach...ty of Washington, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach

  7. Experiment list: SRX089241 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX089241 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 35723144,96.8,9.9,33401 Chromatin accessibility assay of Fetal Stomach MOL...n, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISS

  8. Experiment list: SRX055202 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX055202 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 38031203,94.6,13.8,69544 Chromatin accessibility assay of Fetal Stomach MO...on, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TIS

  9. Experiment list: SRX201805 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX201805 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 84316394,87.7,11.9,53812 Chromatin accessibility assay of Fetal Stomach MO...on, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TIS

  10. Experiment list: SRX100969 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX100969 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 38168446,96.3,11.3,37519 Chromatin accessibility assay of Fetal Stomach MO...on, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TIS

  11. Experiment list: SRX055185 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX055185 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 26520930,92.3,8.7,41280 Chromatin accessibility assay of Fetal Stomach MOL...n, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISS

  12. Experiment list: SRX089271 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX089271 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 33593969,97.4,9.4,36741 Chromatin accessibility assay of Fetal Stomach MOL...n, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISS

  13. Experiment list: SRX055162 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX055162 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 26939006,93.7,7.0,27546 Chromatin accessibility assay of Fetal Stomach MOL...n, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISS

  14. Experiment list: SRX201818 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX201818 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 50367722,78.6,11.1,40553 Chromatin accessibility assay of Fetal Stomach MO...on, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TIS

  15. Experiment list: SRX100995 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX100995 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 42093159,97.5,8.2,34302 Chromatin accessibility assay of Fetal Stomach MOL...n, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISS

  16. Experiment list: SRX201194 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX201194 hg19 DNase-seq DNase-Seq Digestive tract Stomach MeSH Description=An orga...nd the beginning of the DUODENUM. 86597838,84.9,12.6,60171 Chromatin accessibility assay of Fetal Stomach MO...on, Congenital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TIS

  17. Validation of protein evaluation systems by means of milk production experiments with dairy cows.

    NARCIS (Netherlands)

    Straalen, van W.M.; Salaün, C.; Veen, W.A.G.; Rypkema, Y.S.; Hof, G.; Boxem, T.J.

    1994-01-01

    Protein evaluation systems (crude protein (CP), digestible crude protein (DCP), protein digested in the intestine (PDI), amino acids truly absorbed in the small intestine (AAT), absorbed protein (AP), metabolizable protein (MP), crude protein flow at the duodenum (AAS) and digestible protein in inte

  18. Digestive Tract

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    8.1 Stomach and duodenum2004380 Increased expression of heparanase mRNA is correlated with metastasis of gastric carcinoma and is probably correlated with c-met protein. CAIYongguo (蔡永国),et al. 3rd Milk Univ.Dept Gas-troenterol, South-West Hosp, Chongqing 400038. 2004;84(12):974-978.

  19. Smoking and Your Digestive System

    Science.gov (United States)

    ... the risk of H. pylori infection, slows the healing of peptic ulcers, and increases the likelihood that peptic ulcers will recur. The stomach and duodenum contain acids, enzymes, and other substances that help digest food. However, these substances may also harm the lining ...

  20. Expression of digestive enzymes and nutrient transporters in Eimeria-challenged broilers

    Science.gov (United States)

    Avian coccidiosis is a disease caused by the intestinal protozoa Eimeria. The site of invasion and lesions in the intestine is species-specific, for example E. acervulina affects the duodenum, E. maxima the jejunum, and E. tenella the ceca. Lesions in the intestinal mucosa cause reduced feed effic...

  1. Use of a colonoscope for distal duodenal stent placement in patients with malignant obstruction

    NARCIS (Netherlands)

    S.M. Jeurnink (Suzanne); A. Repici (Alessandro); C. Luigiano (Carmelo); N. Pagano (Nico); E.J. Kuipers (Ernst); P.D. Siersema (Peter)

    2009-01-01

    textabstractBackground: Stent placement in the distal duodenum or proximal jejunum with a therapeutic gastroscope can be difficult, because of the reach of the endoscope, loop formation in the stomach, and flexibility of the gastroscope. The use of a colonoscope may overcome these problems. Objectiv

  2. Effects of Food Components That Activate TRPA1 Receptors on Mucosal Ion Transport in the Mouse Intestine.

    Science.gov (United States)

    Fothergill, Linda J; Callaghan, Brid; Rivera, Leni R; Lieu, TinaMarie; Poole, Daniel P; Cho, Hyun-Jung; Bravo, David M; Furness, John B

    2016-10-10

    TRPA1 is a ligand-activated cation channel found in the intestine and other tissues. Components of food that stimulate TRPA1 receptors (phytonutrients) include allyl isothiocyanate, cinnamaldehyde and linalool, but these may also act at other receptors. Cells lining the intestinal mucosa are immunoreactive for TRPA1 and Trpa1 mRNA occurs in mucosal extracts, suggesting that the TRPA1 receptor is the target for these agonists. However, in situ hybridisation reveals Trpa1 expression in 5-HT containing enteroendocrine cells, not enterocytes. TRPA1 agonists evoke mucosal secretion, which may be indirect (through release of 5-HT) or direct by activation of enterocytes. We investigated effects of the phytonutrients on transmucosal ion currents in mouse duodenum and colon, and the specificity of the phytonutrients in cells transfected with Trpa1, and in Trpa1-deficient mice. The phytonutrients increased currents in the duodenum with the relative potencies: allyl isothiocyanate (AITC) > cinnamaldehyde > linalool (0.1 to 300 μM). The rank order was similar in the colon, but linalool was ineffective. Responses to AITC were reduced by the TRPA1 antagonist HC-030031 (100 μM), and were greatly diminished in Trpa1-/- duodenum and colon. Responses were not reduced by tetrodotoxin, 5-HT receptor antagonists, or atropine, but inhibition of prostaglandin synthesis reduced responses. Thus, functional TRPA1 channels are expressed by enterocytes of the duodenum and colon. Activation of enterocyte TRPA1 by food components has the potential to facilitate nutrient absorption.

  3. Iron deficiency anemia as initial presentation of a non-small cell lung carcinoma: A case report

    NARCIS (Netherlands)

    P.V.M. Linsen (Philip V.M.); V.M.J. Linsen (Victor M.J.); G. Buunk (Gerba); D.E. Arnold (Dorothee E.); J.G.J.V. Aerts (Joachim)

    2015-01-01

    textabstractDuodenal metastases secondary to lung cancer are very rare and most of the time asymptomatic. When symptomatic they usually present with bowel obstruction or perforation. We here describe the case of a 68 year-old man with a solitary metastasis in the duodenum from a non-small cell lung

  4. Expression of Secretory Mucins in the Human Upper Gastrointestinal Tract; The Role of MUC5AC in the Adhesion of Helicobacter Pylori

    NARCIS (Netherlands)

    J.H.B. van de Bovenkamp (Jeroen)

    2003-01-01

    textabstractDe bovenste organen van het spijsverteringsstelsel omvatten de oesofagus, maag en duodenum. Deze compartimenten dienen voor transport en tijdelijke opslag van ingenomen voedsel, en initiëren de vertering van voedsel. Ziektes van het bovenste spijsverteringsstelsel zijn een belangrijke

  5. Age-dependent alterations in Ca2+ homeostasis: Role of TRPV5 and TRPV6

    NARCIS (Netherlands)

    M. van Abel (Monique); S. Huybers (Sylvie); J.G. Hoenderop (Joost); A.W.C.M. Kemp (Annemiete); J.P.T.M. van Leeuwen (Hans); R.J.M. Bindels (René)

    2006-01-01

    textabstractAging is associated with alterations in Ca2+ homeostasis, which predisposes elder people to hyperparathyroidism and osteoporosis. Intestinal Ca2+ absorption decreases with aging and, in particular, active transport of Ca2+ by the duodenum. In addition, there are age-related changes in re

  6. Nonsulfated cholecystokinins in the small intestine of pigs and rats

    DEFF Research Database (Denmark)

    Agersnap, Mikkel; Rehfeld, Jens F

    2015-01-01

    -step assay specific for nonsulfated CCK. For further characterization, the intestinal extracts were subjected to size- and ion exchange-chromatography. The intestinal concentrations of sulfated and nonsulfated CCK were highest in the duodenum and the proximal part of jejunum both in the pig and the rat...

  7. Use of antibiotic growth promoter and two herbal natural feed ...

    African Journals Online (AJOL)

    and without exogenous enzymes in wheat based broiler diets .... Vitamin premix* ... **Trace mineral premix/kg diet: Mn - 80 mg; Fe - 60 mg; Zn - 60 mg; Cu - 5 mg; ... end of the duodenum to the ileocaecal junction) was removed from each bird.

  8. Abnormal distribution of the interstitial cells of cajal in an adult patient with pseudo-obstruction and megaduodenum

    DEFF Research Database (Denmark)

    Boeckxstaens, Guy E; Rumessen, Jüri J; de Wit, Laurens

    2002-01-01

    laparoscopic placement of a jejunostomy showed vacuolated myocytes and fibrosis predominantly in the outer third of the circular muscle layer of the duodenum, suggestive for visceral myopathy. The distribution of ICC was also strikingly abnormal: by light microscopy, ICC surrounding the myenteric plexus were...

  9. Download this PDF file

    African Journals Online (AJOL)

    GB

    2016-11-06

    Nov 6, 2016 ... The sections of rat stomach and duodenum given 100mg/kg herbal cocktail and ... Herbal medicines are used as treatment options by as many as 50% of ..... healers in Pakistan as the dosage depends on the type and severity ...

  10. Management of trichobezoar: case report and literature review.

    NARCIS (Netherlands)

    Gorter, R.R.; Kneepkens, C.M.; Mattens, E.C.; Aronson, D.C.; Heij, H.A.

    2010-01-01

    Trichobezoars (hair ball) are usually located in the stomach, but may extend through the pylorus into the duodenum and small bowel (Rapunzel syndrome). They are almost always associated with trichotillomania and trichophagia or other psychiatric disorders. In the literature several treatment options

  11. Management of trichobezoar: case report and literature review

    NARCIS (Netherlands)

    Gorter, R.R.; Kneepkens, C.M.F.; Mattens, E.C.J.L.; Aronson, D.C.; Heij, H.A.

    2010-01-01

    Trichobezoars (hair ball) are usually located in the stomach, but may extend through the pylorus into the duodenum and small bowel (Rapunzel syndrome). They are almost always associated with trichotillomania and trichophagia or other psychiatric disorders. In the literature several treatment options

  12. Duodenal myotomy blocks reduction of meal size and prolongation of intermeal interval by cholecystokinin.

    Science.gov (United States)

    Lateef, Dalya M; Washington, Martha C; Raboin, Shannon J; Roberson, Allison E; Mansour, Mahmoud M; Williams, Carol S; Sayegh, Ayman I

    2012-02-01

    We have shown that vagotomy (VGX) attenuates the reduction of meal size (MS) produced by cholecystokinin (CCK) -8 and -33 and that celiaco-mesenteric ganglionectomy (CMGX) attenuates the prolongation of the intermeal interval (IMI) produced by CCK-33. Here, we report the following novel data. First, by determining the distribution of CCK(1) receptor messenger RNA, which mediates reduction of MS and prolongation of IMI by CCK, in seven regions of the gastrointestinal tract in the adult rat we found that the duodenum contains the highest concentration of this receptor in the gut. Second, based on the previous finding we performed a unique surgical technique known as duodenal myotomy (MYO), which severs all the nerves of the gut wall in the duodenum including vagus, splanchnic and enteric nerves. Third, we determined MS and IMI in duodenal MYO rats in responses to endogenous CCK-58 released by the non-nutrient, trypsin inhibitor, camostat and CCK-8 to test the possibility that the duodenum is the site of action for reduction of MS and prolongation of IMI. We found that, similar to the previous work reported by using CCK-8 and MS, duodenal MYO also blocked reduction of MS by camostat. Forth, duodenal MYO blocked prolongation of IMI by camostat. As such, our current results suggest that the duodenum is the gut site that communicates both feeding signals of endogenous CCK, MS and IMI, with the brain through vagal and splanchnic afferents.

  13. Imaging of common bile duct by linear endoscopic ultrasound

    Institute of Scientific and Technical Information of China (English)

    Malay; Sharma; Amit; Pathak; Abid; Shoukat; Chittapuram; Srinivasan; Rameshbabu; Akash; Ajmera; Zeeshn; Ahamad; Wani; Praveer; Rai

    2015-01-01

    Imaging of common bile duct(CBD) can be done by many techniques. Endoscopic retrograde cholangiopancreaticography is considered the gold standard for imaging of CBD. A standard technique of imaging of CBD by endoscopic ultrasound(EUS) has not been specifically described. The available descriptions mention different stations of imaging from the stomach and duodenum. The CBD lies closest to duodenum and choice of imaging may be restricted to duodenum for many operators. Generally most operators prefer multi station imaging during EUS and the choice of selecting the initial station varies from operator to operator. Detailed evaluation of CBD is frequently the main focus of imaging during EUS and in such situations multi station imaging with a high-resolution ultrasound scanner may provide useful information. Examination of the CBD is one of the primary indications for doing an EUS and it can be done from five stations:(1) the fundus of stomach;(2) body of stomach;(3) duodenal bulb;(4) descending duodenum; and(5) antrum. Following down the upper 1/3rd of CBD can do imaging of entire CBD from the liver window and following up the lower 1/3rd of CBD can do imaging of entire CBD from the pancreatic window. This article aims at simplifying the techniques of imaging of CBD by linear EUS.

  14. [Brunners gland hiperplasia. Report of two cases].

    Science.gov (United States)

    Padilla Ruiz, Maykel

    2014-04-01

    Brunner's gland hyperplasia is an infrequent benign injury located on the first or second portion of the duodenum. The disease spectrum includes diffuse nodular hyperplasia, circumscribed nodular hyperplasia, and Brunner's gland adenoma. We report two cases, one with an adenoma of Brunner's glands as a duodenal polyp and the other as a diffuse nodular hyperplasia of the duodenal bulb.

  15. Diagnosis of Chronic Gastrointestinal Ischemia

    NARCIS (Netherlands)

    D. van Noord (Désirée)

    2011-01-01

    textabstractThree aortic branches provide the arterial blood supply to the gastrointestinal tract: the celiac artery (CA), superior mesenteric artery (SMA) and inferior mesenteric artery (IMA). The CA supplies stomach, liver, part of the pancreas and proximal part of the duodenum. The SMA supplies t

  16. Symptoms and Causes of Peptic Ulcer Disease

    Science.gov (United States)

    ... pylori are spiral-shaped bacteria that can cause peptic ulcer disease by damaging the mucous coating that protects the lining of the stomach and duodenum. Once H. pylori have damaged the mucous coating, powerful stomach acid can get through to the sensitive lining. Together, ...

  17. Safety Study of Photodynamic Therapy Using Talaporfin Sodium in the Pancreas and Surrounding Tissues in the Syrian Golden Hamster

    Directory of Open Access Journals (Sweden)

    Johannes Wittmann

    2014-01-01

    Full Text Available Aim. To assess the safety of photodynamic therapy (PDT using talaporfin sodium on the pancreas and surrounding organs in normal hamsters. Methods. Fluorescence microscopy documented talaporfin levels in liver, duodenum, and pancreas up to 24 hours after photosensitisation. Lesion size in liver 3 days after PDT (50 J, 5 mg/kg, variable drug-light interval (DLI was documented to optimise the DLI. Using optimum DLI, pancreas and surrounding organs were treated with laser fibre touching the surface and animals were killed at 3 or 21 days. Results. Peak fluorescence was seen in duodenum and pancreas at 15 mins (second lower peak at 2 hours. Liver fluorescence was consistently high (peak 1 hour until after 4 hours. Optimum DLI was seen at 15 minutes. The pancreas was relatively resistant to direct PDT injury (small lesions at high doses but surrounding stomach, duodenum, and liver were more susceptible with evidence of adhesions and full thickness damage (localised peritonitis and duodenal perforation at highest doses. Conclusion. The safety profile is similar to PDT with longer acting photosensitisers. The pancreas appears safe to treat, but care is required to avoid high light doses to the intestinal tract, particularly the duodenum.

  18. Radiological aspects of Crohn's disease in small intestine: iconographic assay; Aspectos radiologicos da doenca de Crohn no intestino delgado: ensaio iconografico

    Energy Technology Data Exchange (ETDEWEB)

    Barros, Nestor de; Juliano, Adriana G.; Polizini, Jose M.R.; Rejtman, Debora; Cerri, Giovanni Guido; Rocha, Manoel de Souza [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina. Dept. de Radiologia

    1999-12-01

    The authors present the radiological features of Crohn's disease in small intestine as ways of differential diagnosis of others diseases of duodenum and adjacent organs. In this differentiation or confirmation of Crohn's disease the US and TC have proven to be clinically efficacious in the identification of lesions.

  19. Duodenal versus jejunal biopsies in suspected celiac disease

    NARCIS (Netherlands)

    Thijs, WJ; van Baarlen, J; Kleibeuker, JH; Kolkman, JJ

    2004-01-01

    Background and Study Aims: In the past, small-bowel biopsies for diagnosis of celiac disease were taken from the jejunum with a suction capsule, but nowadays most physicians take endoscopic biopsies from the distal duodenum. To validate that practice we compared the diagnostic yield of endoscopic du

  20. Imaging findings of intragastric gallstone and bouveret's syndrome : case report

    Energy Technology Data Exchange (ETDEWEB)

    Lim, Seong Youb; Seong, Hun; Park, Jong Yeon; Seo, Chang Hye; Jang, Kyung Jae; Cho, Seong Rak [Department of Radiology, Dae Dong Hospital, Pusan (Korea, Republic of)

    2000-01-01

    Gallstone ileus is a well-known complication of cholelithiasis, but is relatively rare. Most ectopic gallstones are located in the small bowel; they are rarely found in the stomach and duodenum. We describe the imaging findings of a case of intragastric gallstone, as well as a case in which duodenal obstruction was caused by a large gallstone (Bouveret's syndrome). (author)

  1. Intestinal permeability of metformin using single-pass intestinal perfusion in rats

    Institute of Scientific and Technical Information of China (English)

    Nai-Ning Song; Quan-Sheng Li; Chang-Xiao Liu

    2006-01-01

    AIM: To characterize the intestinal transport and mechanism of metformin in rats and to investigate whether or not metformin is a substrate for P-glycoprotein (P-gp).METHODS: The effective intestinal permeability of metformin was investigated using single-pass intestinal perfusion (SPIP) technique in male Waster rats. SPIP was performed in three isolated intestinal segments (duodenum, jejunum and ileum) at the same concentration of metformin (50 μg/mL) to test if the intestinal transport of metformin exhibited sitedependent changes, and in a same isolated intestinal segment (duodenal segment) at three different concentrations of metformin (10, 50, 200 μg/mL) to test if the intestinal transport of metformin exhibited concentration-dependent changes. Besides, P-gp inhibitor verapamil (400 μg/mL) was co-perfused with metformin (50 μg/mL) in the duodenum segment to find out if the intestinal absorption of metformin was affected by P-gp exiting along the gastrointestinal track.Stability studies were conducted to ensure that the loss of metformin could be attributed to intestinal absorption.RESULTS: The effective permeability values (Peff) of metformin in the jejunum and ileum at 50 μg/mL were significantly lower than those in the duodenum at the same concentration. Besides, Peff values in the duodenum at high concentration (200 μg/mL) were found to be significantly lower than those at low and medium concentrations (10 and 50 μg/mL). Moreover the coperfusion with verapamil did not increase the Peff value of metformin at 50 μg/mL in the duodenum.CONCLUSION: Metformin could be absorbed from the whole intestine, with the main absorption site at duodenum. This concentration-dependent permeability behavior in the duodenum indicates that metformin is transported by both passive and active carrier-mediated saturable mechanism. The Peff value can not be increased by co-perfusion with verapamil, indicating that absorption of metformin is not efficiently transported by P

  2. [The prognostic value of gastric metaplasia in the duodenal mucosa in patients with Helicobacter pylori positive duodenal bulb ulcer].

    Science.gov (United States)

    Marshalko, O V; Konorev, M R

    2008-01-01

    The predictive value of gastric metaplasia in the duodenal mucosa in patients Helicobacter pylori-positive patients with duodenal bulb ulcer (DBU) was investigated. One hundred and twenty four randomly selected patients with DBU were included in this prospective study. The detection of Helicobacter pylori (HP) in the stomach and duodenum was carried out with Giemsa (using standard visual analogue scale), rapid urease test (standard Jatrox-HP test, Rohm Pharma, Germany), and polymerase chain reaction (PCR) to detect the specific fragment of ureC HP gene (Helicopol II, Lytech, Russia). Regions of gastric metaplasia of the duodenum were confirmed by periodic acid-Schiff and alcian blue (Serva) staining (pH 1.0; 2.5) Duodenal ulcer (DU) complications were registered within 8 to 10 years. Estimation of the predictive factor (gastric metaplasia in the duodenum) was carried out in patients with non-complicated DU (Group 1; n = 73), and with such complications as bleeding, perforation, penetration, pyloroduodenal stenosis (Group 2; n = 51) which were revealed within the 8 to 10 years of observation. Gastric metaplasia in the duodenum was found in 64 or 87.7% of the 73 patients with non-complicated DU and in 5 or 9.8% of the 51 patients with complicated DU within 8 to 10 years of observation. The following facts about the predictive factor for the prognosis of DU complication were found: the sensitivity of 83.6%, the specificity of 92.8%, the predictive accuracy of 88.7%, the relative risk of the predicted outcome of 7.5, the relative risk of a different outcome of 0.11, the odds ration of 65.4. The study revealed a high and significant (p gastric metaplasia in the duodenum as a marker of non-complicated clinical course of DU in HP-positive patients within an 8 to 10-year period.

  3. "Ductal adenocarcinoma in anular pancreas".

    Science.gov (United States)

    Benassai, Giacomo; Perrotta, Stefano; Furino, Ermenegildo; De Werra, Carlo; Aloia, Sergio; Del Giudice, Roberto; Amato, Bruno; Vigliotti, Gabriele; Limite, Gennaro; Quarto, Gennaro

    2015-09-01

    The annular pancreas is a congenital anomaly in which pancreatic tissue partially or completely surrounds the second portion of the duodenum. Its often located above of papilla of Vater (85%), rarely below (15%). This pancreatic tissue is often easily dissociable to the duodenum but there is same cases where it the tissue is into the muscolaris wall of the duodenum. We describe three case of annular pancreas hospitalized in our facility between January 2004 and January 2009. There were 2 male 65 and 69 years old respectively and 1 female of 60 years old, presented complaining of repeated episodes of mild epigastric pain. Laboratory tests (including tumor markers), a direct abdomen X-ray with enema, EGDS and total body CT scan were performed to study to better define the diagnosis. EUS showed the presence of tissue infiltrating the muscle layer all around the first part of duodenum. Biopsies performed found the presence of pancreatic tissue with focal areas of adenocarcinoma. Subtotal gastrectomy with Roux was performed. The histological examinations shows an annular pancreas of D1 with multiple focal area of adenocarcinoma. (T1aN0M0). We performed a follow up at 5 years. One patients died after 36 months for cardiovascular hit. Two patients, one male and one female, was 5-years disease-free. Annular pancreas is an uncommon congenital anomaly which usually presents itself in infants and newborn. Rarely it can present in late adult life with wide range of clinical severities thereby making its diagnosis difficult. Pre-operative diagnosis is often difficult. CT scan can illustrate the pancreatic tissue encircling the duodenum. ERCP and MRCP are useful in outlining the annular pancreatic duct. Surgery still remains necessary to confirm diagnosis and bypassing the obstructed segment. Copyright © 2015 IJS Publishing Group Limited. Published by Elsevier Ltd. All rights reserved.

  4. Effect of citric acid and microbial phytase on small intestinal morphology in broiler chicken

    Directory of Open Access Journals (Sweden)

    Rouhollah Nourmohammadi

    2013-01-01

    Full Text Available An experiment was carried out to investigate the effects of citric acid (CA (0, 3 and 6% and microbial phytase (MP (0, 500 and 1000 IU/kg on morphology of different segments of small intestine (duodenum, jejunum and ileum in broiler chickens fed on corn and soybean meal based diets. The effect of 9 experimental treatments (3×3 factorial design were assessed using 270 7-d-old Ross 308 male broiler chicks in a randomized complete block design in three replicates of 10 birds each. The mean villi length (VL, crypt depth (CD and goblet cell number (GCN in duodenum, jejunum and ileum and villi width (VW and VL:CD ratio in duodenum was significantly greater for the birds fed on acidified diets compared to the control birds at day 42 of age (P<0.01. Inclusion of 3% CA in diet significantly decreased the epithelial thickness (ET in duodenum, jejunum and ileum (P<0.01. The birds received diets with 1000 IU/kg of MP showed significant increase in CD (P<0.01 and GCN in jejunum (P<0.05, and significant decrease in VL:CD ratio and ET in the duodenum (P<0.01, jejunum (P<0.05 and ileum (P<0.01 segments. No variable of interest were affected by CA × MP interaction. It was concluded that CA and MP independently exhibit positive impact on morphometery of small intestine, toward facilitating the nutrient absorption and reducing the metabolic demands of the intestinal tract in broiler chickens.

  5. Human duodenal phase III migrating motor complex activity is predominantly antegrade, as revealed by high-resolution manometry and colour pressure plots.

    Science.gov (United States)

    Andrews, J M; O'donovan, D G; Hebbard, G S; Malbert, C H; Doran, S M; Dent, J

    2002-08-01

    Abstract Late phase III migrating motor complex activity has been said to be primarily retroperistaltic but has not been assessed with high resolution manometry or three-dimensional colour pressure plots (pressure/time/distance). Duodenal phase III was examined in healthy young volunteers (seven male, two female) with a 20-lumen assembly. With the most proximal sidehole in the distal antrum, after a 4.5-cm interval 18 sideholes at 1.5-cm intervals spanned the duodenum with a final sidehole 3 cm beyond. Fasting pressures were recorded until phase III occurred. Comparisons were made between proximal (P) and distal (D) duodenum during early (E) (first 0.5-1 min) and late (L) (last 0.5-1 min) phase III. With colour pressure analysis, 121 of 180 pressure wave (PW) sequences were purely antegrade, two purely retrograde and 57 bidirectional. Ten of fifty-seven bidirectional PW sequences were complex, branching to become two separate sequences. Bidirectional sequences occurred more frequently in late than early phase III (L 43 vs. E 14 of 57), but their occurrence did not differ between proximal and distal duodenum (P31 vs. D 24 of 57). Antegrade propagation velocity was faster in late compared with early phase III (L 28.50 vs. E 17.05 mm s(-1); P = 0.006), but did not differ between proximal and distal duodenum. Colour pressure analysis also indicated an intermittent segmental pattern to phase III, with each subject exhibiting a change in velocity or direction, or a relative failure of peristalsis somewhere along the duodenum during part of phase III. Duodenal phase III is not homogenous and, in contrast with previous studies, does not primarily constitute a retroperistaltic pump. Colour pressure analysis is useful in interpreting intraluminal pressure profiles and may improve the sensitivity and specificity of clinical studies.

  6. Diversity of Duodenal and Rectal Microbiota in Biopsy Tissues and Luminal Contents in Healthy Volunteers.

    Science.gov (United States)

    Li, Gangping; Yang, Min; Zhou, Kan; Zhang, Lei; Tian, Lugao; Lv, Shangze; Jin, Yu; Qian, Wei; Xiong, Hanhua; Lin, Rong; Fu, Yu; Hou, Xiaohua

    2015-07-01

    The diverse microbial communities that colonize distinct segments of the gastrointestinal tract are intimately related to aspects of physiology and the pathology of human health. However, most recent studies have focused on the rectal or fecal microbiota, and the microbial signature of the duodenum is poorly studied. In this study, we compared the microbiota in duodenal and rectal samples to illustrate the characteristic microbial signatures of the duodenum in healthy adults. Nine healthy volunteers donated biopsies and luminal contents from the duodenum and rectum. To determine the composition and diversity of the microbiota, 454- pyrosequencing of bacterial 16S rRNA was performed and multiple bioinformatics analyses were applied. The α-diversity and phylogenetic diversity of the microbiota in the duodenal samples were higher than those of the rectal samples. There was higher biodiversity among the microbiota isolated from rectal biopsies than feces. Proteobacteria were more highly represented in the duodenum than in the rectum, both in the biopsies and in the luminal contents from the healthy volunteers (38.7% versus 12.5%, 33.2% versus 5.0%, respectively). Acinetobacter and Prevotella were dominant in the duodenum, whereas Bacteroides and Prevotella were dominant in the rectum. Additionally, the percentage of OTUs shared in biopsy groups was far higher than in the luminal group (43.0% versus 26.8%) and a greater number of genera was shared among the biopsies than the luminal contents. Duodenal samples demonstrated greater biological diversity and possessed a unique microbial signature compared with the rectum. The mucosa-associated microbiota was more relatively conserved than luminal samples.

  7. Effects of Acute and Chronic Cold Stress on Expression of Cyclooxygenase-2 and Prostaglandin E Synthase mRNA in Quail Intestine

    Directory of Open Access Journals (Sweden)

    J Fu, CP Liu1, ZW Zhang1, W Liao2 and SW Xu1,*

    2013-07-01

    Full Text Available The cold temperature, a common environmental stress, reduces the immunity and re-production activities of the poultry. This study aims to investigate the role of acute and chronic cold exposure in the regulation of cyclooxygenase-2 (COX-2 and prostaglandin E synthase (PTGES expression in the duodenum, jejunum, and ileum of quail. A total of 96 quail with 15 days of age were randomly allocated into 12 groups (8 each group for exposure to acute (up to 12 h and chronic (up to 20 days cold temperature (12±1°C. After that, different segments of the intestine were harvested and subjected to morphology observations under the light and electronic microscopes. qRT-PCR was performed to analyze expression of COX-2 and PTGES, and DNA sequencing was performed to analyze PCR products. The data showed that under acute cold stress, expression of COX-2 and PTGES mRNA was first decreased and then increased in the duodenum, jejunum, and ileum of quail. However, chronic cold stress induced expression of COX-2 and PTGES mRNA in the duodenum, jejunum and ileum of quail, which was then reduced after 20 days of cold exposure. Morphologically, significant changes were also observed in the duodenum, jejunum and ileum after both acute and chronic cold stresses to the animals. The data from the current study indicated that both acute and chronic cold stresses were able to induce inflammation responses in the duodenum, jejunum and ileum, which might be due to the cold-damaged intestinal morphology.

  8. Effects of Acute and Chronic Cold Stress on Antioxidant Function in Intestinal Tracts of Chickens

    Institute of Scientific and Technical Information of China (English)

    Xu Ming; Yu Xian-yi; Li Jin-long; Han Yan-hui; Li Shu; Xu Shi-wen

    2012-01-01

    This study was to investigate the effects of cold stress on the contents of total antioxidant capacity (T-AOC) malondialdehyde (MDA) and superoxide dismutase (SOD) in duodenum, jejunum and ileum of chickens. A total of 80 15-dayold male chickens were treated by cold stress with the duration of the acute cold stress being 1, 3, 6, 12, and 24 h, and the chronic cold stress was 5, 10, and 20 days, respectively. Cold stress temperature was (12±1)℃. The chemical colorimetric method was used to detect the changes of the T-AOC, SOD activities and MDA contents. The results showed that compared with the corresponding control group, effects of acute cold stress on the T-AOC in duodenum, jejunum and ileum of chickens significantly (P〈0.05) increased firstly and then decreased. Under chronic cold stress, the T-AOC significantly (P〈0.05) decreased. Under acute cold stress and chronic cold stress, the MDA contents significantly (P〈0.05) increased in duodenum, jejunum and ileum of chickens. The effects of acute cold stress on the SOD activities in duodenum, jejunum and ileum of chickens significantly (P〈0.05) increased firstly and then decreased Under chronic cold stress the SOD activities significantly (P〈0.05) decreased in jejunum and ileum, but significantly (P〈0.05) decreased firstly and then increased in duodenum.

  9. The peripheral modulation of duodenal and colonic motility in rats by the pancreatic polypeptide-fold family: neuropeptide Y, peptide YY, and pancreatic polypeptide.

    Science.gov (United States)

    Wager-Pagé, S A; Ghazali, B; Anderson, W; Veale, W L; Davison, J S

    1993-01-01

    Neuropeptide Y (NPY), peptide YY (PYY), and pancreatic polypeptide (PP) altered intraluminal pressure in the duodenum and colon of fasted anesthetized rats following intravenous bolus administration. There were rapid increases in intraluminal pressure of the duodenum and colon of anesthetized rats following peripheral injections of NPY, PYY and PP. Administration (IV) of NPY, PYY, and PP increased intraduodenal pressure +1.8, +3.2, and +3.7 mmHg compared to saline baseline. Prazosin, an alpha-2 adrenergic antagonist, did not alter the response of the duodenum of urethane-anesthesized rats to any of the PP-fold peptides following peripheral administration. Yohimbine, an alpha 2-adrenergic antagonist, attenuated the excitatory response of rat duodenum following NPY (IV) but did not alter the duodenal response to PP (IV). Intravenous NPY, PYY, and PP increased intracolonic pressure +2.0, +3.3, and +6.2 mmHg compared to saline baseline. In the presence of prazosin, an alpha 1-adrenergic antagonist, the intraluminal pressure of the colon increased +2.6, +2.4, and +8.1 mmHg compared to saline baseline by NPY, PYY, and PP (IV), respectively. In the presence of alpha 2-adrenergic blockade by yohimbine, NPY, PYY, and PP (IV) increased intraluminal pressure of the colon +4.2, +2.9, and +2.5 mmHg compared to saline baseline. The response of the duodenum to the excitatory effect of PYY (IV) was enhanced in the presence of yohimbine. Duodenal and colonic tone were modulated by the PP-fold peptides following peripheral administration. The alpha-adrenergic nervous system played only a minor role in the modulation of GI motility by the PP-fold peptides at peripheral sites.

  10. Predictive dosimetric parameters for gastrointestinal toxicity with hypofractioned radiotherapy in pancreatic adenocarcinoma

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    Liu X

    2016-04-01

    Full Text Available Xian Liu,* Gang Ren,* Liqin Li, Tingyi Xia Department of Radiation Oncology, Air Force General Hospital, Beijing, People’s Republic of China *These authors contributed equally to this work Abstract: To better guide the development and optimization of radiotherapy planning, to reduce the incidence of radiation reactions, and to improve the quality of life of the patients with pancreatic cancer using radiotherapy, we conducted this study to explore the dosimetric parameters that predict the risk of gastrointestinal (GI toxicity with hypofractioned radiotherapy for pancreatic cancer. Between January 2014 and January 2015, the medical records of 68 patients with pancreatic cancer who underwent helical tomotherapy at the Air Force General Hospital were analyzed. The doses delivered to the planning target volume, clinical target volume, and gross tumor volume–internal gross tumor volume of the primary pancreatic lesions were 50, 60, and 70–80 Gy in 15–20 fractions, respectively. GI toxicity was scored according to version 4.0 of the National Cancer Institute Common Terminology Criteria for Adverse Events. The stomach and duodenum were contoured separately to determine the dose–volume histogram parameters. Univariate and multivariate analyses were adopted to identify clinical and physical risk factors associated with GI toxicity. The median follow-up was 9 months (range: 4–16 months. Eighteen patients had grade II acute GI toxicity, one patient had grade III acute GI toxicity, 17 patients had grade II late GI toxicity, and one patient had grade III late GI toxicity. On univariate analysis, the volume, the average dose Dmean, the maximum dose to 1, 3, 5, and 10 cm3 of the stomach and duodenum (D1, D3, D5, and D10, and the relative volumes receiving 5–40 Gy (V5–V40, and the absolute volumes receiving 5–45 Gy (aV5–aV45 of the duodenum were significantly associated with grade II or higher GI toxicity (P<0.05. On multivariate

  11. Case of radiation gastroduodenitis caused by /sup 60/Co-irradiation therapy for hepatoma

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    Nishikawa, H.; Hayashi, N.; Morise, K.; Tunekawa, J.; Kaneshiro, K. (Nagoya Univ. (Japan). Faculty of Medicine)

    1981-02-01

    A 56-year-old man with hepatoma, who had been treated with total 3,960 rad of /sup 60/Co-irradiation 2 months previously, was readmitted to the hospital because of fever and anemia. Following admission, he passed tarry stools every day. Barium meal examination revealed esophageal varices and erosive gastritis of the antrum. At endoscopy, many hemorrhagic erosions were found in the gastric antrum and the first part of duodenum, which were located in the irradiation area. Since repeated blood transfusion failed to improve anemia, a complete fasting with intravenous hyperalimentation and antacid therapy were started. Two months later, feeding was started and thereafter continued without any appreciable GI bleeding or worsening of anemia. Endoscopic examination at this time revealed only a few erosions scattered over the edematous antral mucosa as well as the proximal duodenum. IVH, antacids and abstinence from food seem to be an effective measure in the treatment of radiation injury of the gut.

  12. Post-operative bariatric surgery complications: Deficiency of nutrients

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    Syed Abdul Majid Mufaqam1, Soni Dhwani Satishkumar2, Patel Palak Arvindkumar2

    2013-08-01

    Full Text Available Since more than half of the population in America falls under the category of obesity, scientists have discovered a surgical technique to reduce the weight of the obese patients. Bariatric surgery or gastric bypass surgery is a procedure that has been successful in reducing the weight for obese people. This technique requires a permanent gastric bypass (Roux-en-Y where part of the stomach and duodenum is removed. Since the size of the stomach is reduced to 20% of its original size along with the removal of duodenum – this may lead to improper absorption of several vitamins and minerals. This review showed that several vitamins and mineral deficiencies are observed in patients, post-operative bariatric surgery. Thiamin, folate, and B12 deficiencies were most commonly observed, and Vitamin A, D, C and B6 deficiencies were also seen in some cases. Iron and calcium deficiencies were also reported by some of the studies.

  13. Duodenal Lipomatosis as a Curious Cause of Upper Gastrointestinal Bleed: A Report with Review of Literature.

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    Zirpe, Dinesh; Wani, Majid; Tiwari, Priyanka; Ramaswamy, Palaniswamy Kalipatti; Kumar, Reddy Prasanna

    2016-05-01

    Lipomas of the gastrointestinal tract are rare. Duodenal lipomas are incidental and mostly asymptomatic. Tumours may produce symptoms of abdominal pain and discomfort or cause bleeding due to ulceration or intestinal obstruction due to intussusception. We describe a 45-year-old man presenting in emergency with 3 days history of melena with normal gastroduodenoscopy and contrast enhanced computed tomography revealing multiple polypoid lesion in duodenum and proximal jejunum suggestive of lipoma. Due to ongoing bleed, he underwent laparotomy with duodenectomy and uneventful postoperative recovery. Our review of cases published in last 67 years indicate that duodenal lipomas are rare to occur but commonly found in second part, they may be seen in third and fourth part of duodenum which may be missed on endoscopy. They can be multiple and may present as severe UGI bleeding which could be managed surgically. Though CT is diagnostic, histopathology confirms the diagnosis which shows lipomatous lesion composed of mature adipose arranged in lobules.

  14. Fatal upper gastrointestinal bleed arising from duodenal varices secondary to undiagnosed portal hypertension.

    Science.gov (United States)

    Larson, John V; Steensma, Elizabeth A; Burke, Leandra H; Bartholomew, David M

    2013-08-16

    Duodenal varices are an unexpected source of upper gastrointestinal haemorrhage associated with high mortality. The prevalence of ectopic variceal bleeding accounts for 2-5% of all variceal bleeding; of this, only 17% occurs in the duodenum. Diagnosis is difficult, and insufficient evidence exists to demonstrate the best treatment option when haemorrhage occurs. We report the case of a 69-year-old man with a history of chronic alcoholism who presented to the emergency department (ED) with nausea, vomiting and several episodes of haematochezia. Diagnostic workup in the ED included CT with multiplanar reconstruction, which revealed a network of large tortuous blood vessels running near the second portion of the duodenum between the inferior vena cava and portal vein. The patient was emergently treated with endoscopic therapy and clipping of the vessel. This failed, and he was subsequently taken to the operating room for suture ligation of the bleeding duodenal varices.

  15. Effect of including liquid vinasse in the diet of rabbits on growth performance

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    Maria Cristina de Oliveira

    2013-04-01

    Full Text Available The effects of liquid vinasse (LV in the diet for growing rabbits on performance, carcass yield and intestinal morphometry were assessed. Eighty New Zealand white rabbits were used in a randomized block design with five treatments (LV inclusion at 0, 25, 50, 75 and 100 g/kg diet and four replications. There was no effect of the treatment on final weight, daily weight gain, mortality rate and carcass yield characteristics. The daily intakes of feed, dry matter, crude protein and energy and feed conversion decreased linearly with increase in LV in the diet. Including LV affected the duodenum crypt depth and the ilium villus perimeter and height linearly and affected the duodenum villus perimeter, height and the absorption surfaces and ilium crypt depth and absorption surface quadratically. There was no effect of including LV on jejunum morphometry. Vinasse can be used to feed growing rabbits at up to 87.8 g per kilogram of diet.

  16. [The absorption and metabolism of oxymatrine in rat intestine].

    Science.gov (United States)

    Cai, Li-yun; Wu, Li-li; Yu, Xiao-ming; Liu, Jun-jin; Han, Wei-chao; Wei, Qiang; Tang, Lan

    2015-10-01

    The purpose of this study is to systematically investigate the characteristics of absorption and metabolism of oxymatrine (OMT) using rat intestinal perfusion model. Ultra performance liquid chromatography (UPLC) and high performance liquid chromatography-electrospray ionization-quadrupole-time of flight mass spectrometry (HPLC-ESI(+)-Q-TOF-MS) were used to test absorption of OMT in intestine at 100, 200 and 400 µmol · L(-1). The absorption rate and permeability of OMT is not dependent on concentration, but through passive absorption in intestine (P > 0.05). In the rat intestine, the absorbed amount of OMT was significantly different in four sections of the intestine in an order of duodenum > jejunum > ileum > colon (P < 0.05). OMT is metabolized into two metabolites in duodenum and jejunum, and matrine (MT) is the major one.

  17. Trichinella spiralis

    DEFF Research Database (Denmark)

    Theodoropoulos, G.; Hicks, S. J.; Corfield, A. P.

    2005-01-01

    Duodenal and jejunal responses to infection with Trichinella spiralis were compared in weaned piglets with a "normal dirty" vs. a "clean SPF" gut flora. Histochemical staining of neutral, acidic, sialylated, and sulphated residues was used to assess biosynthetic responses in mucin-secreting goblet...... in the infected conventional animals than in the infected SPF group. This was accompanied by changes in the pattern of sulphation and sialylation in the duodenum and jejunum. The thickness of the glycocalyx was increased in both duodenum and jejunum in both infected groups. However, this effect was greater...... for the infected SPF animals than the infected conventional animals. No significant differences were observed between uninfected conventional and uninfected SPF pigs....

  18. Pseudomelanosis duodeni in a female adult with chronic renal failure

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    Wei-Chih Sun

    2014-12-01

    Full Text Available Pseudomelanosis duodeni is a rare endoscopic finding that manifests as dark speckled spots in the duodenum. It is considered a benign condition and is associated with certain diseases and the use of certain medications. This study reports a case of a 74-year-old woman, with end-stage renal disease under maintenance hemodialysis, hypertension under regular medical control, iron deficiency anemia under oral iron supplement, and progressive anemia with suspicious occult gastrointestinal bleeding. Upper gastrointestinal endoscopy revealed multiple tiny brownish-black pigmentation throughout the proximal second portion of the duodenum. The histopathological examination showed pigment-laden macrophages with positive iron stain and negative melanin stain in the lamina propria of the mucosal villi.

  19. Economic Burden of Chronic Pancreatitis and Implications of Total Pancreatectomy and Autologous Islet Cell Transplantation

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    Ashley R Dennison

    2008-09-01

    Full Text Available Context Heterotopic pancreas is usually an incidental finding during pathologic evaluation of gastrointestinal polyps or lesions encoun-tered during endoscopy for nonspecific symptoms or unrelated conditions. However, the same neoplastic processes that occur in normal pancreas also can occur in pancreatic heterotopias. Case report We report two cases of intraductal papillary mucinous neoplasms arising in pancreatic heterotopia within the duodenum of two patients. These cases are among the first reports of neoplasia occurring in pancre-atic heterotopia of the duodenum. Both patients are being managed expectantly, as there is currently no consensus regarding the proper follow up in these cases, particularly those that have been incompletely excised. Conclusion These cases highlight the potential for neo-plasia in pancreatic heterotopia and emphasize the importance of careful evaluation of these lesions. Close clinical follow up and possible excision may be warranted in patients with concerning pathologic or clinical findings.

  20. Early Rupture of an Ultralow Duodenal Stump after Extended Surgery for Gastric Cancer with Duodenal Invasion Managed by Tube Duodenostomy and Cholangiostomy

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    Konstantinos Blouhos

    2013-01-01

    Full Text Available When dealing with gastric cancer with duodenal invasion, gastrectomy with distal resection of the duodenum is necessary to achieve negative distal margin. However, rupture of an ultralow duodenal stump necessitates advanced surgical skills and close postoperative observation. The present study reports a case of an early duodenal stump rupture after subtotal gastrectomy with resection of the whole first part of the duodenum, complete omentectomy, bursectomy, and D2+ lymphadenectomy performed for a pT3pN2pM1 (+ number 13 lymph nodes adenocarcinoma of the antrum. Duodenal stump rupture was managed successfully by end tube duodenostomy, without omental patching, and tube cholangiostomy. Close assessment of clinical, physical, and radiological signs, output volume, and enzyme concentration of the tube duodenostomy, T-tube, and closed suction drain, which was placed near the tube duodenostomy site to drain the leak around the catheter, dictated postoperative management of the external duodenal fistula.

  1. Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats

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    Xufeng Han

    2015-09-01

    Full Text Available The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum isolated from goat. Green fluorescent protein (GFP was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.

  2. EFSA CEF Panel (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids), 2015. Scientific Opinion on Flavouring Group Evaluation 208 Revision 1 (FGE.208Rev1): Consideration of genotoxicity data on representatives for 10 alicyclic aldehydes with the a,b-unsaturation in ring / side-chain and precursors from chemical subgroup 2.2 of FGE.19

    DEFF Research Database (Denmark)

    Beltoft, Vibe Meister; Nørby, Karin Kristiane

    genotoxicity studies on p-mentha-1,8-dien-7-al [FL-no: 05.117], the representative substance for FGE.19 subgroup 2.2. This substance was tested in vivo in a combined micronucleus assay in bone marrow and Comet assay in liver and duodenum. It did not induce any increase in micronucleated polychromatic...... erythrocytes of the bone marrow of male rats in the micronucleus test and it did not induce DNA damage in duodenum of the same animals as analysed by the Comet assay. The Comet assay performed in liver shows a positive result and therefore the Panel concluded that p-mentha-1,8-dien-7-al [FL-no: 05...

  3. Scientific opinion of Flavouring Group Evaluation 205 Revision 1 (FGE.205Rev1): consideration of genotoxicity data on representatives for 13 α,β-unsaturated aliphatic ketones with terminal double bonds and precursors from chemical subgroup 1.2.2 of FGE.19

    DEFF Research Database (Denmark)

    Beltoft, Vibe Meister; Nørby, Karin Kristiane

    curve and with a very narrow concentration range resulting in mutagenicity. Both substances were also tested in mammalian cells for gene mutations at the hprt locus and for structural and numerical chromosomal aberrations in the micronucleus assay. Also in mammalian cells, the test substances were...... of the representative substances, pent-1-en-3-one, in an in vivo Comet assay on the first site of contact (e.g. the stomach or duodenum) and on the liver. The Industry has now submitted new data, a combined micronucleus and Comet assay for pent-1-en-3-one, with scoring in the liver and duodenum, and an Ames test...... highly cytotoxic. The Panel considered that the positive effects in the bacterial mutagenicity assays of the two representative substances cannot be overruled by the one negative and one equivocal gene mutation test in mammalian cells. Therefore, the Panel recommended to test the most potent...

  4. Upper gastrointestinal hemorrhage due to duodenal stromal tumor

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    Parreira José Gustavo

    2003-01-01

    Full Text Available BACKGROUND: Gastrointestinal stromal tumor represents a rare neoplasm that originates in the muscular wall of the hollow viscera. AIM: To report gastrointestinal stromal tumor as a source of upper gastrointestinal bleeding, which required urgent surgical control. PATIENT/METHOD: A man with 61 years old was admitted to the emergency service sustaining hematemesis and melena. Endoscopy showed active bleeding from a tumor in the second portion of the duodenum, which was controlled by heater probe cauterization. Surgery was performed through a median laparotomy. A local resection of a 4 cm tumor in the second portion of the duodenum was carried out, together with a primary end-to-end anastomosis and a duodenal diverticulization. No complications happened during the post-operative period. Morphologic examination showed gastrointestinal stromal tumor with no atypical mitosis and a preserved capsule. CONCLUSION: Albeit not being common, gastrointestinal stromal tumors can represent a source of substantial gastrointestinal hemorrhage.

  5. An Unusual Cause of Duodenal Obstruction: Persimmon Phytobezoar.

    Science.gov (United States)

    Fan, Shengxian; Wang, Jing; Li, Yousheng

    2016-12-01

    Duodenal phytobezoar, an unusual cause of acute duodenal obstruction, is rarely seen. The most common cause of this type of bezoar is persimmon. It frequently arises from underlying gastrointestinal tract pathologies (gastric surgery, etc.). Here, we report the case of a 66-year-old man who had undergone distal gastrectomy with Billroth I reconstruction for gastric cancer and experienced severe epigastric discomfort, abdominal pain, and vomiting for a few days. The abdominal computed tomography scan showed a large-sized mass in the horizontal portion of the duodenum. On following endoscopic examination, a large phytobezoar was revealed in the duodenum. He was treated with endoscopic fragmentation combined with nasogastric Coca-Cola. The patient tolerated the procedure well and resumed a normal oral diet 3 days later.

  6. Altered ghrelin secretion in mice in response to diet-induced obesity and Roux-en-Y gastric bypass

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    Aki Uchida

    2014-10-01

    Full Text Available The current study examined potential mechanisms for altered circulating ghrelin levels observed in diet-induced obesity (DIO and following weight loss resulting from Roux-en-Y gastric bypass (RYGB. We hypothesized that circulating ghrelin levels were altered in obesity and after weight loss through changes in ghrelin cell responsiveness to physiological cues. We confirmed lower ghrelin levels in DIO mice and demonstrated elevated ghrelin levels in mice 6 weeks post-RYGB. In both DIO and RYGB settings, these changes in ghrelin levels were associated with altered ghrelin cell responsiveness to two key physiological modulators of ghrelin secretion – glucose and norepinephrine. In DIO mice, increases in ghrelin cell density within both the stomach and duodenum and in somatostatin-immunoreactive D cell density in the duodenum were observed. Our findings provide new insights into the regulation of ghrelin secretion and its relation to circulating ghrelin within the contexts of obesity and weight loss.

  7. Ultrasonographic abdominal anatomy of healthy captive caracals (Caracal caracal).

    Science.gov (United States)

    Makungu, Modesta; du Plessis, Wencke M; Barrows, Michelle; Koeppel, Katja N; Groenewald, Hermanus B

    2012-09-01

    Abdominal ultrasonography was performed in six adult captive caracals (Caracal caracal) to describe the normal abdominal ultrasonographic anatomy. Consistently, the splenic parenchyma was hyperechoic to the liver and kidneys. The relative echogenicity of the right kidney's cortex was inconsistent to the liver. The gall bladder was prominent in five animals and surrounded by a clearly visualized thin, smooth, regular echogenic wall. The wall thickness of the duodenum measured significantly greater compared with that of the jejunum and colon. The duodenum had a significantly thicker mucosal layer compared with that of the stomach. Such knowledge of the normal abdominal ultrasonographic anatomy of individual species is important for accurate diagnosis and interpretation of routine health examinations.

  8. Laparoscopic gastric partitioning gastrojejunostomy for an unresectable duodenal malignant tumor

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    Matsumoto Toshifumi

    2005-01-01

    Full Text Available As a palliative bypass for unresectable gastric or periampullary cancer, gastrojejunostomy (GJ is sometimes associated with postoperative delayed gastric emptying. We report the successful laparoscopic application of this procedure in a 78-year-old man with duodenal obstruction. Computed tomography revealed a mass in the duodenum along with multiple masses in the liver. A radiological image showed an ulcerative tumour in the third portion of the duodenum occluding the lumen. He was diagnosed as having an unresectable duodenal cancer with multiple liver metastases. He needed palliative bypass surgery. Laparoscopically, the stomach was partially divided using an endoscopic autosuture device, and end-to-side GJ was performed successfully. He was given a normal diet on the fourth postoperative day, and there was no delayed gastric emptying. Laparoscopic gastric partitioning GJ is a feasible and safe procedure to prevent postoperative delayed gastric emptying in case of malignant duodenal obstruction.

  9. Meat proteins had different effects on oligopeptide transporter PEPT1 in the small intestine of young rats.

    Science.gov (United States)

    Li, Mengjie; Li, Chunbao; Song, Shangxin; Zhao, Fan; Xu, Xinglian; Zhou, Guanghong

    2016-12-01

    The peptide transporter 1 (PEPT1) in the apical membrane of enterocytes is the central mechanism for regulating the absorption of di- and tripeptides. Dietary proteins may affect PEPT1 abundance and peptide absorption. The present study aimed to characterize changes in PEPT1 mRNA and PEPT1 protein levels in the duodenum and jejunum of young rats after 7-day diet intervention with casein (reference), soy, beef, pork, chicken and fish proteins and further evaluate the impact on the epithelial absorption capacity. RT-PCR and western blot analyses showed that: (1) PEPT1 protein level in duodenum was higher (p  0.05). The soy protein group had lower crypt depth and higher V/C ratio in the jejunum (p absorption capacity as reflected by gut morphology in small intestine.

  10. Gastroduodenal Intussusception Caused by a Peutz- Jeghers Polyp in a Young Child: A Case Report

    Energy Technology Data Exchange (ETDEWEB)

    Roh, Jae Eun; Cho, Bum Sang; Lee, Seung Young; Jeon, Min Hee; Lee, Ji Hyuk; Lee, Ho Chang; Koo, Ji Hae [Chungbuk National University College of Medicine, Cheongju (Korea, Republic of)

    2010-09-15

    Gastroduodenal intussusception is a rare condition caused by the prolapse of a gastric tumor with subsequent invagination of a portion of the gastric wall into the duodenum. Various gastric lesions including adenoma, lipoma, leiomyoma, hamartoma, adenocarcinoma, gastrointestinal stromal tumor (GIST), and as in our case a Peutz- Jeghers polyp, can serve as a lead point of intussusception. Only three cases of gastroduodenal intussusception secondary to a Peutz-Jeghers polyp have been reported. We experienced a case of gastroduodenal intussusception in a 36-month-old boy who presented with intermittent nonbilious vomiting, abdominal pain, and anemia. An abdominal ultrasound and contrast enhanced CT scan showed gastrointestinal intussusception and a mass-like lesion in the second portion of duodenum. A laparotomy revealed a 7x8 cm sized mass at the gastric body, which was pathologically confirmed as a Peutz-Jeghers polyp

  11. Functional characterization of serotonin receptor subtypes in human duodenal secretion

    DEFF Research Database (Denmark)

    Engelmann, Bodil Elisabeth; Bindslev, Niels; Poulsen, Steen Seier;

    2006-01-01

    of dyspeptic patients with or without Helicobacter pylori infection, and to determine the 5-HT receptor subtypes functionally involved. Biopsies from the second part of duodenum were obtained from 43 dyspeptic patients during routine endoscopy. Biopsies were mounted in modified Ussing chambers with air suction......: ketanserin, ondansetron, or SB-204070 (1-butyl-4 piperidinmethyl-8-amino-7-chloro-2,3-dihydro-1,4-benzodioxin-5-carboxylate HCl). Histological examination was performed on duodenal biopsies. Helicobacter urease testing and histological examination determined Helicobacter pylori infection. 5-HT induced a dose......-dependent and bumetanide-sensitive short-circuit current, which was independent of the presence of Helicobacter pylori infection. All the three 5-HT receptor antagonists failed to significantly effect basal and 5-HT-induced short-circuit current. Our results indicate that in human duodenum 1) 5-HT is a potent stimulator...

  12. Obesity And Laboratory Diets Affects Tissue Malondialdehyde (MDA) Levels In Obese Rats

    Science.gov (United States)

    Chowdhury, Parimal; Scott, Joseph; Holley, Andy; Hakkak, Reza

    2010-04-01

    This study was conducted to investigate the interaction of obesity and laboratory diets on tissue malondialdehyde levels in rats. Female Zucker obese and lean rats were maintained on either regular grain-based diet or purified casein diet for two weeks, orally gavaged at day 50 with 65 mg/kg DMBA and sacrificed 24 hrs later. Malondialdehyde (MDA) levels were measured in blood and harvested tissues. Data were recorded as mean ± SEM and analyzed statistically. Results show that the obese group on purified casein diet had reduction of MDA levels in the brain, duodenum, liver, lung and kidney tissues as compared to lean group, p Obese group on grain-based diet showed significant increase in MDA levels only in the duodenum, p obese rats. It appears that purified casein diets were more effective than grain-based diet in reduction of oxidative stress in obese rats.

  13. Penyakit Peptik dan Misoprostol

    Directory of Open Access Journals (Sweden)

    Mariana Raini

    2012-10-01

    Full Text Available Peptic mucus synthesize especially prostaglandine E2 and I2. Prostaglandine E2 and I2 can impede acid secrete and stimulate mucus and bicarbonate secretion. Misoprostof, a synthetic methyl ester analogue of prostaglandin is both a powerful inhibitor of gastric secretion and is able to protect the gastroduodenal mucosa from damage. Misoprostol and other prostaglandine derivatives can be alternative drug of peptic ulcer relapse because the metyl ester of prostaglandin exert an antisecretion and protective effects on peptic mucus. The clinical effectiveness of misoprostol is comparable to cimethidin in short term treatment of peptic and duodenum ulcer as well as reducing duodenal ulcer relapse. Misoprostol may prevent gastrointestinal and peptic ulcer in long term treatment of Non Steroid Anti Jnflamation Drug. The side effect of misoprostol is cervix maturation and uterotonic. This article describe misoprostol effictiveness on peptic and duodenum ulcer treatment.   Key Words: Misoprostol, Peptik ulcer, Sitoproteksi

  14. Acidic duodenal pH alters gene expression in the cystic fibrosis mouse pancreas.

    Science.gov (United States)

    Kaur, Simran; Norkina, Oxana; Ziemer, Donna; Samuelson, Linda C; De Lisle, Robert C

    2004-08-01

    The duodenum is abnormally acidic in cystic fibrosis (CF) due to decreased bicarbonate ion secretion that is dependent on the CF gene product CFTR. In the CFTR null mouse, the acidic duodenum results in increased signaling from the intestine to the exocrine pancreas in an attempt to stimulate pancreatic bicarbonate ion secretion. Excess stimulation is proposed to add to the stress/inflammation of the pancreas in CF. DNA microarray analysis of the CF mouse revealed altered pancreatic gene expression characteristic of stress/inflammation. When the duodenal pH was corrected genetically (crossing CFTR null with gastrin null mice) or pharmacologically (use of the proton pump inhibitor omeprazole), expression levels of genes measured by quantitative RT-PCR were significantly normalized. It is concluded that the acidic duodenal pH in CF contributes to the stress on the exocrine pancreas and that normalizing duodenal pH reduces this stress.

  15. Superior mesenteric artery syndrome following initiation of cisplatin-containing chemotherapy: a case report

    Directory of Open Access Journals (Sweden)

    Ushiki Atsuhito

    2012-01-01

    Full Text Available Abstract Introduction Superior mesenteric artery syndrome is a rare cause of upper intestinal obstruction resulting from compression of the duodenum by the superior mesenteric artery and abdominal aorta. Case presentation We describe a case of superior mesenteric artery syndrome in a 61-year-old Japanese man with non-small cell lung cancer who had been treated with cisplatin-containing chemotherapy and had lost 7 kg in weight. The diagnosis was confirmed by the typical findings of abdominal computed tomography showing distended stomach resulting from compression of the third portion of the duodenum and reduction of an aortomesenteric distance and aortomesenteric angle. Conclusions This case highlights the importance of considering the possibility of superior mesenteric artery syndrome in patients treated with chemotherapy, especially those presenting with a low body mass index and showing weight loss during chemotherapy.

  16. Annular pancreas: the failure of side-to-side gastrojejunostomy anastomosis and the success of repair with Roux-en-Y gastrojejunostomy

    Directory of Open Access Journals (Sweden)

    Isaac A. Deswanto

    2015-07-01

    Full Text Available Annular pancreas is a rare congenital anomaly characterized by a partial or complete encirclement of ectopic pancreas tissue around the duodenum. We report a case of annular pancreas in a 15 day-old infant admitted to the hospital with complaints of profuse and recurrent vomiting and loss of body weight. Non-contrast abdominal X-ray showed a dilated stomach with bubbles formation around the upper abdomen. An obstruction was noted and open laparotomy was performed. Upon laparotomy, pancreatic ring encircled the proximal duodenum causing an obstruction. Side-to-side gastrojejunostomy was performed and passage through the bypass was satisfactory. Four days after the operation, vomiting and bulging abdomen ensued. Contrast abdominal X-ray demonstrated filling defects at the level of obstruction and the anastomosis made. Anastomotic stricture was suspected and thus was corrected using Roux-en-Ygastrojejunostomy procedure. Postoperative course in this patient was satisfactory and patient was discharged after 24 days of hospitalization.

  17. Acute pancreatitis and cholangitis: A complication caused by a migrated gastrostomy tube

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Percutaneous endoscopic gastrostomy (PEG) is generally considered safe with a low rate of serious complications. However, dislocation of the PEG-tube into the duodenum can lead to serious complications.An 86-year old Japanese woman with PEG-tube feeding sometimes vomited after her family doctor replaced the PEG-tube without radiologic confirmation. At her hospitalization, she complained of severe tenderness at the epigastric region and the PEG-tube was drawn into the stomach. Imaging studies showed that the tip of PEG-tube with the inflated balloon was migrated into the second portion of the duodenum, suggesting that it might have obstructed the bile and pancreatic ducts,inducing cholangitis and pancreatitis. After the PEG-tube was replaced at the appropriate position, vomiting and abdominal tenderness improved dramatically and laboratory studies became normal immediately. Our case suggests that it is important to secure PEG-tube at the level of skin, especially after replacement.

  18. Missed duodenal web with foreign body impaction in an operated case of midgut malrotation with Down′s syndrome - A diagnosis delayed by endoscopy

    Directory of Open Access Journals (Sweden)

    Krushnakumar Kesan

    2013-01-01

    Full Text Available "Always look for duodenal obstruction in a case of malrotation", this dictum has to be followed. The patient was operated for midgut malrotation at day 2 of life followed by foreign body removal by upper gastrointestinal endoscopy at 2 years of age. We report atwo and half year male child with Down′s syndrome who presented to us with history suggestive of recurrent upper gastrointestinal tract obstruction. During surgery a post sphincteric duodenum web was confirmed and foreign body (berry seed which had impacted proximal to it was removed. The web was excised and the duodenum, split longitudinally, sutured transversely. The postoperative course was uneventful and patient is asymptomatic at follow up. This case illustrates that diagnosis was delayed for 2΍ years after initial presentation in early neonatal age.

  19. Immunocytochemical evidence for a paracrine interaction between GIP and GLP-1-producing cells in canine small intestine

    DEFF Research Database (Denmark)

    Damholt, A B; Kofod, Hans; Buchan, A M

    1999-01-01

    Glucagon-like-peptide 1 (GLP-1) released from the intestine is considered to be an important incretin. We have recently demonstrated that glucose-dependent insulinotropic peptide (GIP) stimulated GLP-1 secretion from canine ileal L cells in culture. To investigate further the interplay between GLP......-1- and GIP-secreting cells, we set out to determine the exact location and abundance of both cell types throughout the canine intestine. Canine small intestine was subdivided into 15-20 segments and investigated by immunocytochemistry with computer-assisted imaging. The abundance of GIP-, GLP-1......- and somatostatin-immunoreactive cells was determined. GIP-secreting K cells were equally distributed in duodenum and jejunum, with the GLP-1-secreting L cells concentrated in the jejunum (5% duodenum, 73% jejunum and 22% ileum). These results indicated that the middle section of the small intestine containing 69...

  20. Double Primary Tumors-Renal Cell Carcinoma and Duodenal Mucinous Adenocarcinoma

    Directory of Open Access Journals (Sweden)

    Dejan Vuckovic

    2012-12-01

    Full Text Available A 59-year old patient was admited to the Gastroenterology Clinic with the signs of gastrointestinal bleeding. Computerized tomography (CT and a barium-meal radiography revealed a circumferential nodular wall narrowing and incomplete stricture at the D2 part of the duodenum. CT also showed a poorly demarcated mass in the upper and lower poles of the left kidney. During the operation, the whole kidney together with the tumor was removed and also a part of the duodenum. Morphological features of both tumors were typical and distinctive enough to set the diagnosis of two independent primary tumors. The possibility of one being the metastasis of the other was excluded. The diagnosis of double primary malignant neoplasms - renal cell carcinoma and duodenal mucinous adenocarcinoma was made.

  1. Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats.

    Science.gov (United States)

    Han, Xufeng; Wang, Lei; Li, Wei; Li, Bibo; Yang, Yuxin; Yan, Hailong; Qu, Lei; Chen, Yulin

    2015-01-01

    The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.

  2. A challenging case of gastric outlet obstruction (Bouveret's syndrome: a case report

    Directory of Open Access Journals (Sweden)

    Gelrud Andres

    2011-10-01

    Full Text Available Abstract Introduction Bouveret's syndrome is a clinically distinct form of gallstone ileus caused by the formation of a fistula between the biliary tract and duodenum. This case reinforces the need for early recognition and treatment of Bouveret's syndrome, as it is associated with high morbidity and mortality rates. Case presentation An 82-year-old Caucasian woman presented with signs and symptoms of small bowel obstruction. Her laboratory workup showed elevated alkaline phosphatase and amylase levels. Computed tomography of her abdomen revealed pneumobilia, a choledochoduodenal fistula and a gallstone obstructing her distal duodenum. The impacted gallstone could not be extracted endoscopically, so our patient underwent open enterolithotomy successfully. However, the postoperative course was complicated by myocardial infarction, respiratory failure and disseminated intravascular coagulation. She died 22 days after surgery, secondary to cardiopulmonary arrest. Conclusion This case clearly highlights the considerable morbidity and mortality associated with Bouveret's syndrome.

  3. Multidetector-Row CT Findings of a Preduodenal Portal Vein Associated with Polysplenic Syndrome in an Adult: A Case Report

    Energy Technology Data Exchange (ETDEWEB)

    Song, Hyun; Lee, Su Lim; Ku, Young Mi; Chun, Chang Woo [Dept. of Radiology, Uijeongbu St. Mary' s Hospital, The Catholic University of Korea College of Medicine, Uijeongbu (Korea, Republic of)

    2012-03-15

    Preduodenal portal vein (PDPV) is a rare developmental anomaly in which the portal vein runs at the ventral side of the duodenum instead of at an intrapancreatic location. Understanding of this anomaly is of considerable surgical importance, because it may cause unintended portal vein injury during operations involving the gall bladder or duodenum. We recently experienced PDPV associated with polysplenia syndrome in a 49-year-old male patient who complained of persistent epigastric pain. CT portal venography with thin slab maximal intensity projection and volume rendering demonstrated the unique feature of convex anastomosis between the L-shaped mesenterico-portal vein and mesenterico-splenic vein in the mid abdomen. We report here the Multidetector-row CT findings of a rare case of PDPV.

  4. Kaposi's sarcoma of the small intestine after renal transplantation : radiological and endoscopic findings

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hyun; Song, Ha Hun; Kang, Si Won; Shinn, Kyung Sub; Ahn, Byung Min [Taejon St. Mary' s Hospital, the Catholic Univ. of Korea, Taejon (Korea, Republic of)

    1997-03-01

    A case of Kaposi's sarcoma involving the small bowel two years after receiving a renal transplant is described. Immunosuppression had been achieved using cyclosporine A and prednisolone. Lesions extended from the duodenum to the ileum;radiologically, they were demonstrated on small bowel follow-through study and computed tomography as multiple small nodular intraluminal masses with or without central umbilication, and endoscopically, were seen as intramural mucosal elevations with a central crater-like ulceration.

  5. Psittacine proventricular dilatation syndrome in an umbrella cockatoo.

    Science.gov (United States)

    Lutz, M E; Wilson, R B

    1991-06-01

    Psittacine proventricular dilatation syndrome (macaw wasting disease) is a fatal disease of the gastrointestinal tract and, sometimes, the CNS. The disease most often affects macaws and is thought to be of viral origin. An Umbrella cockatoo was referred with signs of neurologic dysfunction. Other unusual findings included flaccid crop, distended duodenum, and acute weight loss. Because treatment has not been reported to be effective, the affected cockatoo was euthanatized to limit spread of the disease.

  6. Mucinous adenocarcinoma of the urinary bladder after long-term duodeno-renal and colovesical fistula--case report.

    Science.gov (United States)

    Petrovic, J; Barisic, G; Krivokapic, Z; Krivokapic, B

    2012-01-01

    Primary adenocarcinoma of the urinary bladder is a rare neoplasm. It accounts for 1-2% of all bladder carcinomas and sometimes may be found in the bladder diverticula. Fistula between duodenum and renal pelvis is another rarity while colovesical fistula is not so uncommon. We present a case of a 40 years old man who had surgery for colovesical and duodenorenal fistula and subsequently developed adenocarcinoma of the urinary bladder.

  7. Intermittent fasting modulates IgA levels in the small intestine under intense stress: a mouse model.

    Science.gov (United States)

    Lara-Padilla, Eleazar; Godínez-Victoria, Marycarmen; Drago-Serrano, Maria Elisa; Reyna-Garfias, Humberto; Arciniega-Martínez, Ivonne Maciel; Abarca-Rojano, Edgar; Cruz-Hernández, Teresita Rocío; Campos-Rodríguez, Rafael

    2015-08-15

    Intermittent fasting prolongs the lifespan and unlike intense stress provides health benefits. Given the role of the immunoglobulin A (IgA) in the intestinal homeostasis, the aim of this study was to assess the impact of intermittent fasting plus intense stress on secretory IgA (SIgA) production and other mucosal parameters in the duodenum and ileum. Two groups of six mice, with intermittent fasting or fed ad libitum for 12weeks, were submitted to a session of intense stress by a bout of forced swimming. Unstressed ad libitum fed or intermittently fasted groups were included as controls. After sacrifice, we evaluated intestinal SIgA and plasma adrenal hormones, lamina propria IgA+ plasma-cells, mRNA expression of polymeric immunoglobulin receptor, α- and J-chains in the liver and intestinal mucosa, as well as pro- (tumor necrosis factor-α, interleukin-6 and Interferon-γ) and anti- (interleukin-2, -4, -10 and transforming growth factor-β) inflammatory cytokines in mucosal samples. Under intense stress, intermittent fasting down- or up-modulated the levels of most parameters in the duodenum and ileum, respectively while up-regulated corticosterone levels without affecting epinephrine. Our data suggest intermittent fasting plus intense stress elicited neuroendocrine pathways that differentially controlled IgA and pIgR expression in duodenum and ileum. These findings provide experimental foundations for a presumable impact of intermittent fasting under intense stress on the intestinal homeostasis or inflammation by triggering or reducing the IgA production in ileum or duodenum respectively.

  8. Gastric Duplication Cyst in Association with Duodenal Atresia in a Neonate

    Science.gov (United States)

    Mirshemirani, Alireza; Roshanzamir, Fatollah; Razavi, Sajad; Sarafi, Mehdi

    2016-01-01

    Concurrence of duodenal atresia and gastric duplication cyst is extremely rare entity. We report a 6-day-old female neonate who presented with neonatal intestinal obstruction. X-ray abdomen showed double bubble sign. At laparotomy, a huge cystic structure attached to greater curvature of the stomach along with duodenal atresia of second part of duodenum was found. The cystic structure was excised and duodeno-duodenostomy performed. Histopathology report confirmed it gastric duplication cyst. PMID:26816679

  9. GLP-1 suppresses gastrointestinal motility and inhibits the migrating motor complex in healthy subjects and patients with irritable bowel syndrome

    DEFF Research Database (Denmark)

    Hellström, P M; Näslund, E; Edholm, T

    2008-01-01

    from 2 (1.5-3) to 1 (0.5-1.5), and motility index from 5.2 +/- 0.2 to 4.0 +/- 0.5. Motility responses to GLP-1 were similar in antrum and duodenum. Presence of the GLP-1 receptor in the gut was verified by reverse transcriptase PCR. In conclusion, the gut peptide GLP-1 decreases motility in the antro...

  10. The effect of cysteamine on the Brunner gland secretion in the rat

    DEFF Research Database (Denmark)

    Poulsen, Steen Seier

    1981-01-01

    in the natural defence of the duodenal mucosa, and this study has been performed to determine the effect of cysteamine and pentagastrin on the Brunner glands in the rat. The proximal duodenum was isolated in situ and drained by a polyethylene tube. The secretion was studied for two 5-h periods after...... that cysteamine interferes with the synthesis of the secretory product. The depression of the Brunner gland secretion may be an important factor in the pathogenesis of cysteamine-induced duodenal ulceration....

  11. Low-residue diet fed to rabbits induces histomorphological and biomechanical remodeling of small intestine

    DEFF Research Database (Denmark)

    Liu, Y; Zhao, J; Liao, D;

    2016-01-01

    . Segments from duodenum, jejunum and ileum were excised. The intestinal diameter and length were obtained from digitized images of the segments at preselected luminal pressure levels and at no-load and zero-stress states. Circumferential and longitudinal stresses (force per area) and strains (deformation...... confirmed that the wall thickness decreased in all three segments, which was primarily due to mucosal thinning (PFeeding the low...

  12. 18F-FDG PET/CT in a seldom case of primary duodenal dermatofibrosarcoma protuberans with lung and skeletal metastases.

    Science.gov (United States)

    Karunanithi, Sellam; Jain, Tarun Kumar; Singh, Ashok; Bal, Chandrasekhar; Kumar, Rakesh

    2015-02-01

    Dermatofibrosarcoma protuberans (DFSP) is an uncommon cutaneous soft tissue sarcoma tumor that arises from the dermis of the skin. Sarcomas of the intestines are rare, and a DFSP arising from the small intestine is a very rare occurrence. Here we present F-FDG PET/CT imaging findings of a 60-year-old man with DFSP in the duodenum with metastases to the lung, mesentery, and skeleton.

  13. [The role of biliary dyskinesia in the mechanism of the damage to the protective properties of the mucosal barrier in peptic ulcer].

    Science.gov (United States)

    Oparin, A G; Demerchian, T I; Korenovskiĭ, I P; Chonka, V Iu; Iakovenko, E L; Pandeĭ, I

    1993-01-01

    Mucous barrier resistance was studied in 232 duodenal ulcer patients with signs of exacerbation as shown by PAS-positive substances and acid mucopolysaccharides in antral mucosa of the stomach and duodenum as well as by gastric juice levels of gastromucoproteins, fucose and sialic acids. In addition, measurements were performed of biliary motility. The tests indicated that half of the ulcer patients developed biliary dyskinesia manifesting as hyperkinesia or hypokinesia. The resultant decrease in gastric mucins requires individual correction.

  14. [Operative treatment of complicated duodenal and pyloric ulcer disease].

    Science.gov (United States)

    Oparin, S O; Korotkyï, V M; Kolosovych, I V; Spitsyn, R Iu; Furmanenko, M F; Kartashov, B T; Martynovych, L D; Krasovs'kyĭ, V O; Butyrin, S O; Zinchenko, I I; Rupitsev, O O; Dzhurko, M G

    2000-11-01

    There were examined 135 patients with perforative ulcer of the gastric terminal portion (GTP) and of duodenum. Performance of duodeno- or gastroduodenoplasty without vagotomy, the correcting therapy conduction in early postoperative period had promoted the normalization of the GTP motor function and the gastric acid output reduction in late follow-up period, trusting the expediency of organ-preserving operation conduction without vagotomy as radical method of the complicated ulcer disease treatment.

  15. Endoscopic resection of a huge Brunner's gland adenoma

    Institute of Scientific and Technical Information of China (English)

    Binbin ZHANG; Xu REN; Xiufen TANG; Yuxin CHI; Xuesong SHI

    2008-01-01

    Brunner's gland adenoma is a rare tumour of the duodenum, which is usually benign. A 71-year-old woman presenting with epigastric pain, upper gastro-intestinal haemorrhage and melaena was reported in this paper. Upper gastro-intestinal (GI) endoscopy revealed a large pedunculated tumour on the superior part of the duodenal bulb. Endoscopic polypectomy was successfully performed by clipping and nylon thread without any com-plications. Histological examination revealed a Brunner's gland adenoma.

  16. Relationship between Gastrointestinal Peptides, Intestinal Wall Compliance, and Vascular Resistance

    Science.gov (United States)

    1983-01-20

    elevations in pan - creatic secretions which were both enzyme and bicarbonate rich. These responses could be reproduced by Intravenous Infusions of low doses...Increases in blood flow to the duodenum, jejunum, and pan - creas (all three vascular beds are perfused by this artery). Yet, al- though the superior...Immunohisto- chemical localization of a vasodilatory polypeptide (VIP) in cerebro - vascular nerves. Brain Res. 113: 400-404, 1976, 135. Larsson, L,-I., J

  17. [Heterotopic pancreatic tissue in the bifurcation of the bile duct : Rare diagnosis mimicking a Klatskin tumour].

    Science.gov (United States)

    Heer, C; Pförtner, M; Hamberger, U; Raute-Kreinsen, U; Hanraths, M; Bartsch, D K

    2010-02-01

    Heterotopic tissue of the pancreas can be found in 1-2% of autopsies. The main locations are stomach, duodenum, jejunum, Meckel's diverticulum, and ileum. Less frequently it is observed in the liver, gallbladder, common bile duct, and papilla of Vateri. We report the first case of heterotopic pancreatic tissue in the bifurcation of the bile duct mimicking a Klatskin's tumour. The case is discussed based on the current literature.

  18. Gallbladder cancer manifesting as recurrent common bile duct stone and duodenal ulcer bleeding.

    Science.gov (United States)

    Tsai, Tzung-Jiun; Lai, Kwok-Hung; Hsu, Ping-I; Tsai, Cheng-Chung; Fu, Ting-Ying

    2009-08-01

    Gallbladder cancer (GBC) is an uncommon disease and is usually asymptomatic. Poor prognosis and high mortality rate have been noted in patients with delayed diagnosis. We report a case of locally advanced GBC with duodenum and colon invasion manifesting as bleeding duodenal ulcer and recurrent common bile duct (CBD) stones. The patient was successfully treated with extended surgery. In patients who have multiple recurrence of CBD stones without common risk factors, concomitant biliary tract malignancy should be included in the differential diagnosis.

  19. The protease-activated receptor-2 agonist induces gastric mucus secretion and mucosal cytoprotection

    OpenAIRE

    Kawabata, Atsufumi; Kinoshita, Mitsuhiro; Nishikawa, Hiroyuki; Kuroda, Ryotaro; Nishida, Minoru; Araki, Hiromasa; Arizono, Naoki; Oda, Yasuo; Kakehi, Kazuaki

    2001-01-01

    Protease-activated receptor-2 (PAR-2), a receptor activated by trypsin/tryptase, modulates smooth muscle tone and exocrine secretion in the salivary glands and pancreas. Given that PAR-2 is expressed throughout the gastrointestinal tract, we investigated effects of PAR-2 agonists on mucus secretion and gastric mucosal injury in the rat. PAR-2–activating peptides triggered secretion of mucus in the stomach, but not in the duodenum. This mucus secretion was abolished by pretreatment with capsai...

  20. Helical CT of the abdomen: whole milk as a low-density oral contrast agent; Tomografia helicoidal do abdome: avaliacao do leite integral como contraste oral de baixa densidade

    Energy Technology Data Exchange (ETDEWEB)

    Collares, Felipe Birchal; Diniz, Renata Lopes Furletti Caldeira; Motta, Emilia Guerra Pinto Coelho; Moreira, Wanderval; Ribeiro, Marcelo Almeida [Hospital Mater Dei, Belo Horizonte, MG (Brazil). Dept. de Radiologia

    2000-08-01

    We evaluated 90 abdominal helical computed tomography scans from patients who received 1% iodine solution, whole milk or no oral contrast agent before scanning. Four parameters were evaluated: gastrointestinal distension, mural visualization, pancreas-duodenum discrimination and bowel loop discrimination. Better results were obtained with the use of whole milk compared to iodine contrast or no oral contrast agent. Whole milk is an effective low density oral contrast agent. (author)

  1. Amino acid availability in ruminants of cereals and cereal co-produtcs

    OpenAIRE

    Gonzalez Cano, Javier; J.M. Arroyo; Guevara González, Jesús Alberto; Mohubi, Rabiaa; Piquer, Olga; Moya, Vicente Javier

    2014-01-01

    [EN] BACKGROUND: Microbial correctedin situ estimates of the ruminal undegraded fraction (RU) and intestinal effective digestibility (IED) of amino acids (AA), except tryptophan, of rye, wheat and corn grains, wheat bran, wheat and barley distilled dried grains and corn gluten feed were measured on three rumen- and duodenum-cannulated wethers using 15N-labelling techniques and considering ruminal rates of particle comminution and outflow. RESULTS: The lack of microbial correction ...

  2. Ultrasonographic thickening of the muscularis propria in feline small intestinal small cell T-cell lymphoma and inflammatory bowel disease

    OpenAIRE

    2013-01-01

    Gastrointestinal lymphoma is the most common form of lymphoma in the cat. More recently, an ultrasonographic pattern associated with feline small cell T-cell gastrointestinal lymphoma has been recognized as a diffuse thickening of the muscularis propria of the small intestine. This pattern is also described with feline inflammatory bowel disease. To evaluate the similarities between the diseases, we quantified the thickness of the muscularis propria layer in the duodenum, jejunum and ileum of...

  3. Acrylamide-induced prenatal programming of intestine structure in guinea pig.

    Science.gov (United States)

    Tomaszewska, E; Dobrowolski, P; Puzio, I; Prost, L; Kurlak, P; Sawczuk, P; Badzian, B; Hulas-Stasiak, M; Kostro, K

    2014-02-01

    Potential effects of prenatal administration of acrylamide (ACR) on postnatal development of the small intestine were not examined experimentally yet. The aim of this study was to establish changes of morphological parameters of the small intestine damaged by prenatal action of ACR in guinea pigs. The 3 mg/kg body weight of ACR was given in drinking water every day during the last 35 days of the pregnancy in guinea pigs. The histomorphometry of the duodenum and jejunum was determined. Immunohistochemical staining with anti cadherin antibody was performed. Maternal treatment with ACR led to the decrease of the expression of cadherin in the epithelium. Maternal ACR treatment increased the number of total, divided and inactive crypt, and the number of damaged villi in the duodenum and jejunum of newborn guinea pigs. The thickness of myenteron and submucosa, mucosa fractal dimension and the depth of crypts in the duodenum were increased by ACR. Additionally, in offspring born by mothers administered with ACR the decrease of villi epithelium thickness and active crypt number was observed. Moreover, ACR decreased goblet cells and inact villi number in the duodenum, mucosa thickness and crypts width in the jejunum. Intestine absorptive surface was affected by ACR in the jejunum as well. Results of measurements showed that maternal ACR treatment had negative influence on small intestine histomorphometry. ACR acting prenatally influenced small intestine nervous plexuses that became enlarged by 2.5 times compared with the control group. In conclusion, our results showed the negative impact of maternal ACR treatment on histological structure, integrity and innervation of small intestine wall as well as on absorptive function of small intestine mucosa.

  4. Histomorphometric changes of small intestine in pregnant rat

    OpenAIRE

    2015-01-01

    Food intake of rats increases during pregnancy. This requires changes in the structure of the small intestine to absorb additional food. The aim of the present study was to investigate the morphological changes in the layers of small intestine in rats during pregnancy. Duodenum, jejunum and ileum of 18 pregnant Sprague-Dawley rats (day 7, 14 and 21 of pregnancy) were collected. Villous height and width and thickness of lamina propria, tunica muscularis entirely and separately (circular and lo...

  5. Infection by Mycobacterium avium intracellulare in AIDS: endoscopic duodenal appearance mimicking Whipple's disease.

    Science.gov (United States)

    Vázquez-Iglesias, J L; Yañez, J; Durana, J; Arnal, F

    1988-09-01

    We report the case of a 24-year-old woman who presented with diarrhea, weight loss and abdominal lymph node enlargement. A diagnosis of infection by Mycobacterium avium intracellulare with a clinical picture similar to Whipple's disease was established. The endoscopic study of the duodenum revealed multiple yellow nodules that became confluent in the second portion, entirely replacing the normal mucosa. These endoscopic findings have not been described previously in intestinal infection by Mycobacterium avium intracellulare.

  6. [The semiotics of the pain and dyspeptic syndromes in motor disorders of the digestive organs in children and adolescents].

    Science.gov (United States)

    Dmytriieva, S M

    1999-06-01

    Overall 304 children and adolescents with gastro-duodenal pathology were studied for some aspects of clinical manifestations of the pain and dyspeptic syndromes as related to the character of disordered gastroduodenal motility by making use of techniques of phase polygastroduodenometry. Pathogenetic interrelationship was disclosed of clinical manifestations of the pain and dyspeptic syndromes according to the variant of gastroduodenal dysmotility (dysphasic hyper- or hypomotile dyskinesia of the stomach and duodenum).

  7. Intestinal morphometric and biomechanical changes during aging in rats

    DEFF Research Database (Denmark)

    Zhao, Jingbo; Gregersen, Hans

    2015-01-01

    computed from the length, diameter and pressure data and from the zero-stress state geometry. Results: The duodenal and ileal dimensions increased slightly from 6 to 22 months, e.g. the weight per unit length, the wall thickness and the wall cross-sectional area increased 20%, 4% ,and 25% for duodenum......-strain curves shifted to the left after 18 months (pprocess. Furthermore, the intestinal wall was stiffer in the longitudinal direction than...

  8. The effects of a ration change from a total mixed ration to pasture on rumen fermentation, volatile fatty acid absorption characteristics, and morphology of dairy cows

    NARCIS (Netherlands)

    Schären, M.; Seyfang, G.M.; Steingass, H.; Dieho, K.; Dijkstra, J.; Hüther, L.; Frahm, J.; Beineke, A.; Soosten, von D.; Meyer, U.; Breves, G.; Dänicke, S.

    2016-01-01

    To investigate the effect of the change from a concentrate and silage-based ration (total mixed ration, TMR) to a pasture-based ration, a 10-wk trial (wk 1-10) was performed, including 10 rumen- and duodenum-fistulated German Holstein dairy cows (182 ± 24 d in milk, 23.5 ± 3.5 kg of milk/d; mean

  9. A case of invasive hemolymphangioma of the pancreas

    Institute of Scientific and Technical Information of China (English)

    Yoshikazu Toyoki; Kenichi Hakamada; Shunji Narumi; Masaki Nara; Daisuke Kudoh; Keinosuke Ishido; Mutsuo Sasaki

    2008-01-01

    Hemolymphangioma of the pancreas is a very rare benign tumor. There were only five reports of this disease until March 2008. Herein, we report a case of hemolymphangioma of the pancreas with gastrointestinal bleeding due to duodenal invasion. A 53-year-old man had been admitted a referral hospital because of severe anemia due to gastrointestinal bleeding in December 2005. He was then transferred to our institute with a diagnosis of a tumor of the head of the pancreas with duodenal invasion in January 2006. No abnormalities were revealed except for anemia in laboratory data including CEA and CA19-9. Gastrointestinal endoscopy revealed bleeding at the duodenum. Computed tomography also demonstrated a heterogenous mass at the pancreatic head and suspected invasion to the duodenal wall. Ultrasonography showed a huge mass at the pancreatic head with a mixture of high and low echoic areas. Pylorous-preserving pancreatoduodenectomy was performed. The pancreatic tumor was soft and had invaded to the duodenum. The pathological diagnosis was a hemolymphangioma of the pancreas invaded to the duodenum. His postoperative course was uneventful and he was discharged on the 26th d after surgery.Hemolymphangioma of the pancreas is a very rare benign tumor. In a literature review until March 2008, we found five case reports. Major symptoms are abdominal pain and distension due to the enlarged tumor. However,we experienced a case of hemolymphangioma of the pancreas with gastrointestinal bleeding due to invasion to the duodenum. This disease is a very rare entity, but should be considered when patients have gastrointestinal bleeding.

  10. Extra-Gastrointestinal Stromal Tumor of Retroperitoneal Origin: A Case Report

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Seung Joon; Kim, Hyung Sik; Park, Yul Ri; Choi, Hye Young [Dept. of Radiology, Gachon Medical Center, Gachon University of Medicine and Science, Incheon (Korea, Republic of)

    2012-03-15

    Extragastrointestinal stromal tumors (EGIST) are relatively rare, and cases originating in the retroperitoneum even rarer. We report a 60-year-old woman who presented with an EGIST originating in the retroperitoneum. Computed tomography results demonstrated a soft tissue mass on the right side of the retroperitoneum. The tumor abutted the duodenum, head of the pancreas, and right kidney. The mass was surgically proven to be a retroperitoneal tumor and histopathologically proven to be a retroperitoneal EGIST.

  11. Mapping of liver-enriched transcription factors in the human intestine

    Institute of Scientific and Technical Information of China (English)

    Frank; Lehner; Ulf; Kulik; Juergen; Klempnauer; Juergen; Borlak

    2010-01-01

    AIM: To investigate the gene expression pattern of hepatocyte nuclear factor 6 (HNF6) and other liverenriched transcription factors in various segments of the human intestine to better understand the differentiation of the gut epithelium. METHODS: Samples of healthy duodenum and jejunum were obtained from patients with pancreatic cancer whereas ileum and colon was obtained from patients undergoing right or left hemicolectomy or (recto)sigmoid or rectal resection. All surgical specimens were subjected to his...

  12. [Cancer of the organs of the pancreatoduodenal region (based on autopsy data from 2 hospitals in the city of Donetsk)].

    Science.gov (United States)

    Mikhaĭlichenko, V A

    1979-01-01

    The incidence of cancer of the pancreatoduodenal organs (the pancreas, major duodenal papilla, extrahepatic bile ducts, duodenum) is reported according to the autopsy findings obtained at two clinical hospitals (provincial and municipal) of Donetsk city during the period from 1957 to 1973. There is a tendency to the increased mortality due to cancer of the localization concerned against the background of the reduced total mortality.

  13. Experiment list: SRX252723 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX252723 hg19 No description NA Digestive tract Stomach MeSH Description=An organ ... the beginning of the DUODENUM. 39832625,71.4,8.8,14845 Histone H3K27me3 ChIP-Seq of Fetal Stomach MOLECULE=...genital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISSUE_DEP

  14. Experiment list: SRX252737 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX252737 hg19 No description NA Digestive tract Stomach MeSH Description=An organ ... the beginning of the DUODENUM. 36422495,95.4,9.4,31490 Histone H3K4me3 ChIP-Seq of Fetal Stomach MOLECULE=g...enital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISSUE_DEPO

  15. Experiment list: SRX252731 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX252731 hg19 No description NA Digestive tract Stomach MeSH Description=An organ ... the beginning of the DUODENUM. 35288627,88.4,3.9,13292 Histone H3K4me1 ChIP-Seq of Fetal Stomach MOLECULE=g...enital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISSUE_DEPO

  16. Experiment list: SRX252720 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX252720 hg19 No description NA Digestive tract Stomach MeSH Description=An organ ... the beginning of the DUODENUM. 51023402,79.3,13.1,56942 Histone H3K27ac ChIP-Seq of Fetal Stomach MOLECULE=...genital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISSUE_DEP

  17. Dosimetric Advantages of Midventilation Compared With Internal Target Volume for Radiation Therapy of Pancreatic Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Lens, Eelco, E-mail: e.lens@amc.uva.nl; Horst, Astrid van der; Versteijne, Eva; Tienhoven, Geertjan van; Bel, Arjan

    2015-07-01

    Purpose: The midventilation (midV) approach can be used to take respiratory-induced pancreatic tumor motion into account during radiation therapy. In this study, the dosimetric consequences for organs at risk and tumor coverage of using a midV approach compared with using an internal target volume (ITV) were investigated. Methods and Materials: For each of the 18 patients, 2 treatment plans (25 × 2.0 Gy) were created, 1 using an ITV and 1 using a midV approach. The midV dose distribution was blurred using the respiratory-induced motion from 4-dimensional computed tomography. The resulting planning target volume (PTV) coverage for this blurred dose distribution was analyzed; PTV coverage was required to be at least V{sub 95%} >98%. In addition, the change in PTV size and the changes in V{sub 10Gy}, V{sub 20Gy}, V{sub 30Gy}, V{sub 40Gy}, D{sub mean} and D{sub 2cc} for the stomach and for the duodenum were analyzed; differences were tested for significance using the Wilcoxon signed-rank test. Results: Using a midV approach resulted in sufficient target coverage. A highly significant PTV size reduction of 13.9% (P<.001) was observed. Also, all dose parameters for the stomach and duodenum, except the D{sub 2cc} of the duodenum, improved significantly (P≤.002). Conclusions: By using the midV approach to account for respiratory-induced tumor motion, a significant PTV reduction and significant dose reductions to the stomach and to the duodenum can be achieved when irradiating pancreatic tumors.

  18. Experiment list: SRX252741 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX252741 hg19 No description NA Digestive tract Stomach MeSH Description=An organ ... the beginning of the DUODENUM. 52725451,83.2,20.3,14116 Histone H3K9me3 ChIP-Seq of Fetal Stomach MOLECULE=...genital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISSUE_DEP

  19. Experiment list: SRX252726 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX252726 hg19 No description NA Digestive tract Stomach MeSH Description=An organ ... the beginning of the DUODENUM. 44990767,90.7,2.6,15126 Histone H3K36me3 ChIP-Seq of Fetal Stomach MOLECULE=...genital Defects Lab. Ian Glass || BIOMATERIAL_TYPE=Primary Tissue || TISSUE_TYPE=Fetal Stomach || TISSUE_DEP

  20. Endovascular treatment of a Superior Mesenteric Artery Syndrome variant secondary to traumatic pseudoaneurysm

    Directory of Open Access Journals (Sweden)

    Abercrombie John F

    2010-03-01

    Full Text Available Abstract Pseudoaneurysms related to the superior mesenteric artery (SMA are a recognised complication of trauma to the vessel, and successful treatment with stenting has been previously described. We report the case of a patient who presented with obstruction of the fourth part of the duodenum secondary to a traumatic pseudoaneurysm, a hitherto unreported variant of superior mesenteric artery syndrome. Exclusion of the pseudoaneurysm and relief of the duodenal obstruction were simultaneously achieved by placement of a covered stent.

  1. Acute pancreatitis due to ascariasis: a sonographic pictorial essay on four cases.

    Science.gov (United States)

    Lynser, Donboklang; Thangkhiew, R S; Laloo, Demitrost; Hek, M D; Marbaniang, Evarisalin; Tariang, Satisfy

    2016-06-01

    Ascariasis is the commonest helminthic disease to infect humans. Due to their wandering nature, the roundworms from the second part of the duodenum migrate through the biliary opening into the hepatobiliary and pancreatic ducts. Ascariasis is the most common parasitic cause of pancreatitis in endemic region. Pancreatitis can result due to pancreatic ascariasis, biliary ascariasis or both. Pancreatitis due to ascariasis can be severe and life-threatening. We present a pictorial essay of acute pancreatitis due to ascariasis on four cases.

  2. Síndrome da artéria mesentérica superior Superior mesenteric artery syndrome

    OpenAIRE

    Haberlandh Sodré Lima; Accyoli Moreira Maia; Antonio Kneipp Pitta de Castro Neto

    2000-01-01

    The Superior Mesenteric Syndrome is a rare and controversial disease. The compression of the duodenum by the mesenteric artery and aorta causes an intermitent obstruction. Preoperative diagnosis is very difficult.We present one case of this syndrome in a pacient with severe weight loss and signs of high intestinal obstruction. The diagnosis was based on clinical and radiologic findings. A duodenojejunostomy was performed after medical treatment failure.This patient died on the 20th posoperati...

  3. Microflora of Digestive Tract in Poultry

    OpenAIRE

    ALBAZAZ, Rojan I.; BÜYÜKÜNAL BAL, Emel Banu

    2014-01-01

    Abstract: The gut flora are microorganisms that normally live in digestive tract and can do a number of benefit functions for their host health and growth. The unicellular microorganisms are bacteria (predominant), fungi and protozoans. The intestine divided into three parts: the duodenum and small intestine where the number of bacteria relatively low, <108 g-1, the caeca where the microbial fermentation occurs and the number of bacteria present is approximately 1011 g-1 (wet weight) and t...

  4. A Case of Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia

    OpenAIRE

    SUZUKI, manabu; Onchi, Miyako; Ozaki, Masakazu

    2013-01-01

    Feline gastrointestinal eosinophilic sclerosing fibroplasia was diagnosed in an 8-month-old Scottish fold that had a primary gastrointestinal mass involving the stomach, duodenum and mesenteric lymph nodes. Histopathologically, the most characteristic feature of this mass was granulation tissue with eosinophil infiltration and hyperplasia of sclerosing collagen fiber. Immunohistochemically, large spindle-shaped cells were positive for smooth muscle actin and vimentin. This case emphasizes the...

  5. Dissolution of Lipid-Based Matrices in Simulated Gastrointestinal Solutions to Evaluate Their Potential for the Encapsulation of Bioactive Ingredients for Foods

    OpenAIRE

    Yves Raymond; Champagne, Claude P.

    2014-01-01

    The goal of the study was to compare the dissolution of chocolate to other lipid-based matrices suitable for the microencapsulation of bioactive ingredients in simulated gastrointestinal solutions. Particles having approximately 750 μm or 2.5 mm were prepared from the following lipid-based matrices: cocoa butter, fractionated palm kernel oil (FPKO), chocolate, beeswax, carnauba wax, and paraffin. They were added to solutions designed to simulate gastric secretions (GS) or duodenum secretions ...

  6. Flow of endogenous and exogenous nitrogen in different segments of the small intestine in pigs fed diets with soyabean concentrate, soyabean meal or rapeseed cake

    NARCIS (Netherlands)

    Grala, W.; Buraczewska, L.; Wasilewko, J.; Verstegen, M.W.A.; Tamminga, S.; Jansman, A.J.M.; Huisman, J.; Korczynski, W.

    1998-01-01

    Six barrows of an average initial BW 27.5±1.2 kg were used. Each pig was fitted with two cannulas in different segments of the small intestine: pig 1 in the duodenum (C1) and upper jejunum (C2), pig 2 in C2 and the lower jejunum (C3), pig 3 in C2 and C3, pig 4 in C1 and the terminal ileum (C4), pig

  7. High incidence of biliary complications in rat liver transplantation: Can we avoid it?

    Institute of Scientific and Technical Information of China (English)

    Guo-Lin Li; Hao-Ming Lin; Tian-Zhu Long; Li-Hong Lv; Jian-Dong Yu; Yong-Heng Huang; Jun Min; Yun-Le Wan

    2011-01-01

    IM: To investigate how to reduce the incidence of biliary omplications in rat orthotopic liver transplantation. ETHODS: A total of 165 male Wistar rats were randomly ivided into three groups: Group A, orthotropic iver transplantation with modified "two-cuff" technique; roup B, bile duct was cut and reconstructed without ransplantation; and Group C, only laparotomy was performed. Based on the approaches used for biliary reconstruction, Group A was divided into two sub-groups: A1 (n = 30), duct-duct reconstruction, and A2 (n = 30), duct-duodenum reconstruction. To study the influence of artery reconstruction on bile duct complication, Group B was divided into four sub-groups: B1 (n = 10), duct-duct reconstruction with hepatic artery ligation, B2 (n = 10), duct-duct reconstruction without hepatic artery ligation, B3 (n = 10), duct-duodenum reconstruction with hepatic artery ligation, and B4 (n = 10), duct-duodenum reconstruction without hepatic artery ligation. The samples were harvested 14 d after operation or at the time when significant biliary complication was found. RESULTS: In Group A, the anhepatic phase was 13.7 ± 1.06 min, and cold ischemia time was 50.5 ± 8.6 min. There was no significant difference between A1 and A2 in the operation duration. The time for biliary reconstruction was almost the same among all groups. The success rate for transplantation was 98.3% (59/60). Significant differences were found in the incidence of biliary complications in Groups A (41.7%), B (27.5%) and C (0%). A2 was more likely to have biliary complications than A1 (50% vs 33.3%). B3 had the highest incidence of biliary complications in Group B. CONCLUSION: Biliary complications are almost inevitable using the classical "two cuff" techniques, and duct-duodenum reconstruction is not an ideal option in rat orthotopic liver transplantation.

  8. Effect of Intraperitoneal Radiotelemetry Instrumentation on Voluntary Wheel Running and Surgical Recovery in Mice

    Science.gov (United States)

    2012-09-01

    small transmitter group; model G2 Emitter, Mini Mitter, Bend, OR). Surgical preparation con- sisted of shaving the abdominal fur and scrubbing the...Sl , No 5 September 2012 Pages 600-608 Effect of Intraperitoneal Radiotelemetry Instrumentation on Voluntary Wheel Running and Surgical Recovery...of the diaphragm, large intestine, and duodenum. These results demonstrate that the large transmitter delayed surgical recovery, disrupted normal

  9. Intestinal amyloidosis:Two cases with different patterns of clinical and imaging presentation

    Institute of Scientific and Technical Information of China (English)

    Pier; Paolo; Mainenti; Sabrina; Segreto; Marcello; Manci

    2010-01-01

    The involvement of the small bowel in systemic forms of amyloidosis may be diffuse or very rarely focal.Some cases of focal amyloidomas of the duodenum and jejunum without extraintestinal manifestations have been reported.The focal amyloidomas consisted of extensive amyloid infiltration of the entire intestinal wall thickness.Radiological barium studies,ultrasound and computed tomography(CT)patterns of diffuse small bowel amyloidosis have been described:the signs are non-specific and may include small-bowel...

  10. Effects of Food Components That Activate TRPA1 Receptors on Mucosal Ion Transport in the Mouse Intestine

    Directory of Open Access Journals (Sweden)

    Linda J. Fothergill

    2016-10-01

    Full Text Available TRPA1 is a ligand-activated cation channel found in the intestine and other tissues. Components of food that stimulate TRPA1 receptors (phytonutrients include allyl isothiocyanate, cinnamaldehyde and linalool, but these may also act at other receptors. Cells lining the intestinal mucosa are immunoreactive for TRPA1 and Trpa1 mRNA occurs in mucosal extracts, suggesting that the TRPA1 receptor is the target for these agonists. However, in situ hybridisation reveals Trpa1 expression in 5-HT containing enteroendocrine cells, not enterocytes. TRPA1 agonists evoke mucosal secretion, which may be indirect (through release of 5-HT or direct by activation of enterocytes. We investigated effects of the phytonutrients on transmucosal ion currents in mouse duodenum and colon, and the specificity of the phytonutrients in cells transfected with Trpa1, and in Trpa1-deficient mice. The phytonutrients increased currents in the duodenum with the relative potencies: allyl isothiocyanate (AITC > cinnamaldehyde > linalool (0.1 to 300 μM. The rank order was similar in the colon, but linalool was ineffective. Responses to AITC were reduced by the TRPA1 antagonist HC-030031 (100 μM, and were greatly diminished in Trpa1−/− duodenum and colon. Responses were not reduced by tetrodotoxin, 5-HT receptor antagonists, or atropine, but inhibition of prostaglandin synthesis reduced responses. Thus, functional TRPA1 channels are expressed by enterocytes of the duodenum and colon. Activation of enterocyte TRPA1 by food components has the potential to facilitate nutrient absorption.

  11. Toxic Hazards Research Unit

    Science.gov (United States)

    1977-09-01

    Spleen Trachea Kidneys Lungs and bronchi Adrenals Heart Bladder Thyroid Seminal vesicles Parathyroids Prostate Esophagus Testes Stomach Ovaries Duodenum...20 2 16 Lungs 18 38 24 Liver 4 6 10 Kidneys 3 4 2 Spleen 0 1 0 In both cases, the organ findings were miscellaneous in nature and generally represent...ponents included methane, nitrogen, ammonia , water, methanol, azomethane and hydrazine. Other peak identifications derived from the computer on the basis

  12. Survival of pathogenic and lactobacilli species of fermented olives during simulated human digestion

    OpenAIRE

    Francisco Noé eArroyo López; Stephanie eBlanquet-Diot; Sylvain eDenis; Jonathan eThevenot; Sandrine eChalancon; Monique eAlric; Francisco Rodriguez Gomez; Verónica eRomero-Gil; Rufino eJimenez Diaz; Antonio eGarrido Fernández

    2014-01-01

    The present survey uses a dynamic gastric and small intestinal model to assess the survival of one pathogenic (Escherichia coli O157:H7 EDL 933) and three lactobacilli bacteria with probiotic potential (Lactobacillus rhamnosus GG, Lactobacillus pentosus TOMC-LAB2 and Lactobacillus pentosus TOMC-LAB4) during their passage through the human gastrointestinal tract using fermented olives as the food matrix. The data showed that the survival of the E. coli strain in the stomach and duodenum was ve...

  13. Digestion and absorption of olive oil

    OpenAIRE

    Bermúdez, Beatriz; Pacheco, Yolanda M.; López, Sergio; Abia, Rocío; Muriana, Francisco J.G.

    2004-01-01

    Olive oil is a monounsaturated (oleic acid)-rich fat, mainly constituted by triglycerides (>98%) and minor compounds. As other macronutrients, dietary triglyceride digestion and absorption are a complex processes involving enzyme activities and physicochemical changes. In humans, hydrolysis of olive oil triglycerides begins in the stomach where it is catalyzed by an acid-stable gastric lipase. Triglyceride hydrolysis continues in the duodenum, by the synergetic actions of gastric and...

  14. Digestion and absorption of olive oil

    OpenAIRE

    Muriana, Francisco J.G.; Abia, Rocío; López, Sergio; Pacheco, Yolanda M.; Bermúdez, Beatriz

    2004-01-01

    Olive oil is a monounsaturated (oleic acid)-rich fat, mainly constituted by triglycerides (>98 %) and minor compounds. As other macronutrients, dietary triglyceride digestion and absorption are a complex processes involving enzyme activities and physicochemical changes. In humans, hydrolysis of olive oil triglycerides begins in the stomach where it is catalyzed by an acid-stable gastric lipase. Triglyceride hydrolysis continues in the duodenum, by the synergetic actions of gastric and colipas...

  15. Chronic Inhalation Toxicity of Hydrazine: Oncogenic Effects

    Science.gov (United States)

    1981-06-01

    showed signs of anorexia and general fatigue. Changing diets and forced feedings resulted in the survival of the remaining two dogs. A 6-month chronic...Esophagus Bladder Stomach Seminal vesicles Duodenum Prostate Jejunum Testes Ileum Ovaries Cecum Uterus Colon Nasal cavity Rectum Brain Mesenteric lymph... malnutrition during chronic exposure. Based upon these studies, the current OSHA Threshold Limit Value of 1.0 ppm for hydrazine is unsatisfactory while the

  16. Upper gastrointestinal obstruction secondary to aortoduodenal syndrome owing to a noninflammatory abdominal aortic aneurysm.

    LENUS (Irish Health Repository)

    Cahill, Kevin

    2012-01-31

    Aortoduodenal syndrome is a rare complication of an abdominal aortic aneurysm wherein the aneurysm sac obstructs the patient\\'s duodenum. It presents with the symptoms of an upper gastrointestinal tract obstruction and requires surgical intervention to relieve it. Previously, gastric bypass surgery was advocated, but now aortic replacement is the mainstay of treatment. We report a case of a 67-year-old woman whose aortoduodenal syndrome was successfully managed and review the literature on this topic.

  17. A dosimetric comparison of proton and photon therapy in unresectable cancers of the head of pancreas

    Energy Technology Data Exchange (ETDEWEB)

    Thompson, Reid F.; Zhai, Huifang; Both, Stefan; Metz, James M.; Plastaras, John P.; Ben-Josef, Edgar, E-mail: Edgar.Ben-Josef@uphs.upenn.edu [University of Pennsylvania, Philadelphia, Pennsylvania 19104 (United States); Mayekar, Sonal U. [Thomas Jefferson University, Philadelphia, Pennsylvania 19107 (United States); Apisarnthanarax, Smith [University of Washington, Seattle, Washington 98109 (United States)

    2014-08-15

    Purpose: Uncontrolled local growth is the cause of death in ∼30% of patients with unresectable pancreatic cancers. The addition of standard-dose radiotherapy to gemcitabine has been shown to confer a modest survival benefit in this population. Radiation dose escalation with three-dimensional planning is not feasible, but high-dose intensity-modulated radiation therapy (IMRT) has been shown to improve local control. Still, dose-escalation remains limited by gastrointestinal toxicity. In this study, the authors investigate the potential use of double scattering (DS) and pencil beam scanning (PBS) proton therapy in limiting dose to critical organs at risk. Methods: The authors compared DS, PBS, and IMRT plans in 13 patients with unresectable cancer of the pancreatic head, paying particular attention to duodenum, small intestine, stomach, liver, kidney, and cord constraints in addition to target volume coverage. All plans were calculated to 5500 cGy in 25 fractions with equivalent constraints and normalized to prescription dose. All statistics were by two-tailed paired t-test. Results: Both DS and PBS decreased stomach, duodenum, and small bowel dose in low-dose regions compared to IMRT (p < 0.01). However, protons yielded increased doses in the mid to high dose regions (e.g., 23.6–53.8 and 34.9–52.4 Gy for duodenum using DS and PBS, respectively; p < 0.05). Protons also increased generalized equivalent uniform dose to duodenum and stomach, however these differences were small (<5% and 10%, respectively; p < 0.01). Doses to other organs-at-risk were within institutional constraints and placed no obvious limitations on treatment planning. Conclusions: Proton therapy does not appear to reduce OAR volumes receiving high dose. Protons are able to reduce the treated volume receiving low-intermediate doses, however the clinical significance of this remains to be determined in future investigations.

  18. The endogenous protein content of ruminant proximal duodenal digesta

    OpenAIRE

    Bartram, Christopher Gordon

    1987-01-01

    Protein arriving at the ruminant proximal duodenum consists of microbial protein, undegraded feed protein and endogenous protein. In this study, endogenous protein is defined as that fraction of the digesta derived from the animal itself (e.g. enzymes, plasma proteins, sloughed cells and mucus), not including any endogenous protein which may have been incorporated into the microorganisms. Recent feeding schemes (e.g. ARC 1980, 1984) require an accurate value of the degradability of feed i...

  19. Cholecystitis of a duplicated gallbladder complicated by a cholecystoenteric fistula

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Brady K. [University of Rochester Medical Center, Department of Imaging Sciences, Rochester, NY (United States); Chess, Mitchell A. [University of Rochester Medical Center, Department of Imaging Sciences, Rochester, NY (United States); Advanced Imaging, Batavia, NY (United States)

    2009-04-15

    Gallbladder duplications are uncommon anatomic variants that are sometimes mistaken for other entities on imaging. We present a surgically confirmed case of cholecystitis in a ductular-type duplicated gallbladder complicated by the formation of an inflammatory fistula to the adjacent duodenum. Both US and magnetic resonance cholangiopancreatography were performed preoperatively, in addition to intraoperative cholangiography, which confirmed the presence of a duplicated gallbladder. (orig.)

  20. Effects of PYY on the interdigestive migrating myoelectric complex in the small intestine in vivo and the neural and endocrinal mechanisms of the effects

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Objective To investigate the effects of peptide YY (PYY) on the interdigestive migrating myoelectric complex (MMC) in the small intestine in vivo and explore the neural and endocrinal mechanisms of the effects. Methods Sprague-Dawley rats were supplied with a venous catheter and bipolar electrodes in the duodenum and jejunum for electromyography of stomach and small intestine in wake state. PYY,phentolamine,nitro-L-arginine (L-NNA,the inhibitor of nitric oxide synthase) and atropine were served with PYY res...

  1. Complications of percutaneous endoscopic gastrostomy-jejunostomy for levodopa/carbidopa infusion in advanced Parkinson's disease

    Directory of Open Access Journals (Sweden)

    Ana Olivares

    2012-03-01

    Full Text Available Continuous infusions of levodopa directly into the duodenum-yeyunum is an effective therapy to reduce daily off time in an advanced stage of Parkinson's disease, but it is not without complications, particularly device related problems. We present our experience in three Spanish hospitals with these complications, including a severe case with migration, several decubitus ulcers, and one case of perforation of the bowel and finally death.

  2. Avaliação dos mecanismos de ação envolvidos na atividade antiulcerogênica de Serjania marginata frente á doença ulcerosa péptica experimental

    OpenAIRE

    Périco, Larissa Lucena [UNESP

    2014-01-01

    Peptic ulcer disease includes both gastric and duodenal ulcers and has been a major threat to the world’s population over the past two centuries. The complex and multifactorial pathogenesis of this disease occurs due to an imbalance between aggressive (hydrochloric acid, pepsin, ethanol, NSAIDs, stress, Helicobacter pylori, among others) and protective factors (mucus, bicarbonate, prostaglandins, blood flow) of the mucosa of stomach or duodenum. In an attempt to protect the gastric mucosa fro...

  3. Expression of nesfatin-1/NUCB2 in rodent digestive system

    Institute of Scientific and Technical Information of China (English)

    Yutaka; Oomura

    2010-01-01

    AIM: To observe the regional distributions and morphological features of nesfatin-1/nucleobindin-2 (NUCB2) immunoreactive (IR) cells in the rodent digestive system. METHODS: Paraffin-embedded sections of seven organs (pancreas, stomach, duodenum, esophagus, liver, small intestine and colon) dissected from sprague-dawley (SD) rats and institute of Cancer Research (ICR) mice were prepared. The regional distributions of nesfatin-1/NUCB2 IR cells were observed by immunohistochemical staining. The morphological ...

  4. Robotic Nephroureterectomy with Partial Duodenectomy for Invasive Ureteral Tumor

    OpenAIRE

    Dangle,Pankaj P.; Moore, Stephen; Abaza, Ronney

    2010-01-01

    Robotic surgery is gaining acceptance in the management of diverse urological disorders. Any minimally invasive procedure carries a risk of open conversion either for complications or unexpected intraoperative findings, but the additional dexterity of robotic instrumentation may allow even complex situations to be managed laparoscopically. We report the case of an upper tract transitional cell carcinoma discovered at the time of robotic nephroureterectomy to be invading the duodenum that was ...

  5. Is herpes simplex virus associated with peptic ulcer disease?

    OpenAIRE

    Löhr, J M; Nelson, J. A.; Oldstone, M B

    1990-01-01

    To test the hypothesis that herpes simplex virus type 1 (HSV-1) may be associated with peptic ulcer disease, we examined ulcerative lesions of the distal stomach and proximal duodenum for the presence of nucleic acids and antibodies specific for HSV-1. Utilizing in situ hybridization, immunocytochemistry, and polymerase chain reaction with sequencing, gastric or duodenal tissues from 4 of 22 patients (18%) with documented peptic ulcer disease demonstrated the presence of both specific HSV-1 n...

  6. Endoscopic excision of a prolapsing malignant polyp which caused intermittent gastric outlet obstruction

    Institute of Scientific and Technical Information of China (English)

    Huqh J Freeman

    2005-01-01

    A 69-year-old male with chronic lymphocytic leukemia presented with iron deficiency anemia and post-prandial abdominal fullness. Endoscopy showed a large polyp on a stalk, protruding through the pylorus into the duodenum causing intermittent gastric outlet obstruction. While prolapsing gastric antral polyps are usually benign and hyperplastic, inflammatory or regenerative in type, excisional snare polypectomy here led to complete resolution of his symptoms, but showed a malignant polyp.

  7. Duodenal ileus caused by a calf feeding nipple in a cow

    OpenAIRE

    2011-01-01

    Abstract Background The aim of this report was to describe duodenal obstruction caused by a rubber foreign body in a cow. Case Presentation The clinical, biochemical and ultrasonographic findings in a five-year-old Swiss Braunvieh cow with duodenal ileus caused by a calf feeding nipple are described. The main clinical signs were anorexia, ruminal tympany, decreased faecal output and abomasal reflux syndrome. Ultrasonographic examination revealed reticular hyperactivity and a dilated duodenum....

  8. Immunohistochemical and morphometric analysis of intestinal full-thickness biopsy samples from cats with lymphoplasmacytic inflammatory bowel disease.

    Science.gov (United States)

    Marsilio, S; Kleinschmidt, S; Nolte, I; Hewicker-Trautwein, M

    2014-05-01

    The distribution and numbers of CD3(+) T lymphocytes, immunoglobulin(+) plasma cells and calprotectin (L1)(+) macrophages was analyzed in full-thickness, formalin-fixed biopsy samples from the small intestine (duodenum, jejunum and ileum) and from the colon from nine cats with clinical signs of inflammatory bowel disease (IBD). All animals had lymphoplasmacytic enteritis or lymphoplasmacytic enterocolitis. Equivalent samples from the same intestinal regions from 12 healthy pet cats served as controls. Labelled cells in the lamina propria were counted by computer-aided morphometry. The different cell types were similarly distributed in both groups, but there were differences in their numbers. There were more CD3(+) T cells in the duodenum and jejunum of cats with IBD; however, the difference was only significant for the duodenum. There were significantly more IgA(+) cells in the duodenal crypt region. There were significantly more IgG(+) cells in the lower jejunal crypt region. Plasma cells expressing IgM were decreased in cats with IBD, but the difference was not significant. L1(+) macrophages were significantly decreased in the lower crypt area of the colon in cats with IBD and there was a trend to decreased L1(+) cells in the upper crypt area of the duodenum and jejunum. Comparison of the results of this study with previous findings on endoscopically-obtained duodenal biopsy samples from cats with IBD revealed some differences. These discrepancies might relate to differences between control cat populations, types of biopsy samples, methodological factors such as different counting techniques and the activity of the disease at the time of sampling.

  9. Another new variant of Bouveret's syndrome

    Institute of Scientific and Technical Information of China (English)

    Seong-Heum Park; Sang-Woo Lee; Tae-Jin Song

    2009-01-01

    Although Bouveret's syndrome, i.e. gastric outlet obstruction by a large gallstone impacted in the proximal duodenum secondary to a cholecystoduodenal fistula,is rare, its pathogenesis and clinical features are well characterized. However, existence of variant forms of the syndrome are not well known, and as far as we have been described in the English-language literature.We present a case of another new variant of Bouveret's syndrome in a 54-year-old Korean woman.

  10. Massive rectal bleeding from acquired jejunal diverticula

    OpenAIRE

    2011-01-01

    Abstract Small bowel diverticulosis is an uncommon and often asymptomatic condition that is sporadically observed during radiographic examination or laparotomy. Although it is frequently seen in duodenum, jejunal and ileal locations are very rare. The majority of patients with jejunal diverticula have no symptoms. However, they can present with a number of acute and emergent complications with a high rate of mortality. Bleeding from jejunal diverticula occurs in less than 3% - 8% of patients ...

  11. Antispasmodic effects of Aloysia polystachya and A. gratissima tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by acethylcholine and calcium

    OpenAIRE

    Consolini,Alicia E.; Berardi, Andrea Beatriz; Rosella, María Adelaida; Volonté, María Guillermina

    2011-01-01

    The antispasmodic effects of acqueous extracts (AE) and tinctures (T) of Aloysia polystachya (Griseb.) Moldenke and Aloysia gratissima (Gillies & Hook.) Tronc., Verbenaceae, were studied on rat isolated ileum and duodenum. These plants are used for gastrointestinal disorders and as eupeptic in South America. Both AE non-competitively inhibited the dose-response curves (DRC) of ACh and the DRC of Ca2+ in high-[K+]o, as well as the T. The T of A. polystachya and A. gratissima respectively inhib...

  12. Antispasmodic effects of Aloysia polystachya and A. gratissima tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by acethylcholine and calcium

    OpenAIRE

    Consolini,Alicia E.; Andrea Berardi; Rosella,María A.; María Volonté

    2011-01-01

    The antispasmodic effects of acqueous extracts (AE) and tinctures (T) of Aloysia polystachya (Griseb.) Moldenke and Aloysia gratissima (Gillies & Hook.) Tronc., Verbenaceae, were studied on rat isolated ileum and duodenum. These plants are used for gastrointestinal disorders and as eupeptic in South America. Both AE non-competitively inhibited the dose-response curves (DRC) of ACh and the DRC of Ca2+ in high-[K+]o, as well as the T. The T of A. polystachya and A. gratissima respectively i...

  13. Megaduodenum og obstipation efter operation for kongenit tyndtarmsatresi

    DEFF Research Database (Denmark)

    Karstensen, John; Raahave, Dennis; Kirkegaard, Preben

    2011-01-01

    A 33 year-old female presented with constipation and a right-sided palpable abdominal mass, suspected to be the colon. The colonic transit time was prolonged, but the dilated organ was a mega duodenum that had developed after surgery for three jejunal atresies performed one day after birth....... The colon was malrotated, being situated to the left of the columna. Renewed resection surgery was uneventful, and after such surgery the constipation receded and the colonic transit time returned to normal....

  14. Ultrasound in the diagnosis of gallstone ileus: A case report

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jong Hwa; Lee, Hyeon Kyeong; Lee, Chae Kyeong; Lee, Sung Woo; Lee, Jun Hee [Dongguk University College of Medicine, Seoul (Korea, Republic of)

    1999-06-15

    Gallstone ileus is a relatively uncommon complication of gallbladder stone. It accompanies the fistula from gallbladder to small intestine, usually to duodenum with the mechanical obstruction of small bowel. The stone is most commonly located in the distal ileum. Its diagnosis can be made when the stone is detected in the obstructed bowel lumen by ultrasound. Here we report one case of gallstone ileus diagnosed by ultrasound and confirmed surgically.

  15. Effects of oregano essential oil supplementation to diets of broiler chicks with delayed feeding after hatching. Morphological development of small intestine segments

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    Şenay Sarıca

    2014-04-01

    Full Text Available The study aimed to investigate the effects of dietary supplementation of oregano essential oil (OEO on the morphological development of small intestine of broilers with different feeding times (immediate, 24, 48 or 72 h posthatching delayed feeding from d 0 to 14. The diets were supplemented with: no, 250 or 500 mg/kg of the OEO (OEO250 and OEO500, respectively. Fasting for 72 h significantly increased the weight and length of small intestine segments of broilers on d 14. The OEO250 and OEO500 significantly increased the jejunum villus height of chickens fed immediately and the duodenum villus height of broilers fasted for 48 h. The duodenum villus surface area of chickens fasted for 48 h and the ileum villus surface area of broilers fasted for 24 h were significantly increased by the OEO250. The OEO500 significantly enhanced the duodenum villus surface area of broilers fasted for 24 h and their ileum villus surface area fasted for 48 h. The crypt depths of small intestine segments of broilers fasted for 72 h were significantly reduced by OEO250 and OEO500. In conclusion, the dose of phenolic compounds in OEO reaching the small intestine might be enough for protecting the intestinal epithelial cells from damages of toxins and for removing the negative effects of delayed feeding on the morphological development of all the small intestine segments of broiler chicks on d 14.

  16. Comparison of routinely used intestinal biopsy forceps in dogs: an ex vivo histopathological approach.

    Science.gov (United States)

    Dahan, J; Semin, M O; Monton, C; Amiriantz, S; Concordet, D; Raymond-Letron, I; Dossin, O

    2017-03-01

    To compare the quality of duodenal and ileal samples obtained with different biopsy forceps. Fifteen dogs were included in a prospective ex vivo study. After euthanasia, the duodenum and the ileum were sampled with four different forceps and evaluated according to a standardised scoring system. The biopsy forceps evaluated had alligator jaws or cups with smooth edge with or without a needle. The global quality of the biopsies was better in the ileum that in the duodenum regardless of the biopsy forceps. Biopsy forceps with smooth edge including a needle resulted in fewer artefacts than biopsy forceps with smooth edge but no needle in both sites and those with alligator jaws without a needle provided deeper biopsies than those with smooth edge without a needle only in the duodenum. There was no effect of the biopsy forceps type on the size of the biopsies. Our findings may aid in choosing the appropriate type of forceps for intestinal biopsy. © 2017 British Small Animal Veterinary Association.

  17. Brunner’s Gland Hamartoma: A Rare Cause of Gastrointestinal Bleeding – Case Report and Review of the Literature

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    David R Stolpman

    2002-01-01

    Full Text Available An unusual cause of upper gastrointestinal bleeding is described in a previously healthy 45-year-old man who was admitted to hospital with weakness and fatigue, and had experienced an episode of melena two days before admission. His medical and surgical history was unremarkable. Upon admission to hospital, he showed evidence of iron-deficiency anemia, with a hemoglobin concentration of 61 g/L (normal range 135 to 175 g/L, a mean corpuscular volume of 73 fL (normal range 85.0 to 95.0 fL and a ferritin concentration of 1.0 µg/L (normal range in males 15 to 400 µg/L. Upper gastrointestinal endoscopy revealed a 3.5 cm ulcerated submucosal mass in the third portion of the duodenum, for which mucosal biopsies were nondiagnostic. A subsequent endoscopic ultrasound revealed a 2.7×4.0 cm hyperechoic, cystic, submucosal tumour in the third portion of the duodenum. Endoscopic ultrasound-guided fine needle aspiration revealed no malignant cells. The patient eventually underwent a resection of the third portion of his duodenum. Surgical pathology revealed that this tumour was a Brunner’s gland hamartoma, 4.5 cm in its greatest dimension.

  18. Robotic-assisted repair of a duodenal diaphragm in a child

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    Jennifer H. Myszewski

    2015-08-01

    Full Text Available The retroperitoneal location of the duodenum and the small volume of the peritoneal cavity in pediatric patients make the laparoscopic repair of congenital duodenal anomalies challenging. As a result, robotic-assisted repair of duodenal atresia in a pediatric patient has been reported only once in the literature. This report describes the robotic-assisted laparoscopic repair of a congenital duodenal diaphragm in a 2-year-old, 8 kg patient who presented with vomiting and failure to thrive. An upper gastrointestinal series revealed partial obstruction at the second part of the duodenum with proximal dilatation. These findings are consistent with a duodenal diaphragm. Traditional laparoscopy was utilized to kocherize the first and second parts of the duodenum and to identify a loop of proximal jejunum for the proposed anastomosis. A duodeno-jejunal anastomosis was then performed using intra-corporeal suturing with a daVinci SI robotic system. The patient had a quick and uneventful post-operative course. At 6 month follow-up, she was asymptomatic and the surgical incisions had healed with excellent cosmetic appearance. A combination of laparoscopic and robotic techniques offers a promising alternative to open or purely laparoscopic repair of congenital duodenal anomalies.

  19. Different effects of ghrelin, des-acyl ghrelin and obestatin on gastroduodenal motility in conscious rats

    Institute of Scientific and Technical Information of China (English)

    Mineko Fujimiya; Aldhiro Asakawa; Koji Ataka; Ikuo Kato; Akio Inui

    2008-01-01

    Three peptides, ghrelin, des-acyl ghrelin and obestatin are derived from a common prohormone, preproghre-lin by posttranslational processing, originating from endocrine cells in the stomach. To examine the effects of these peptides, we applied the manometric mea-surement of gastrointestinal motility in freely moving conscious rat models. Ghrelin exerts stimulatory ef-fects on the motility of antrum and duodenum in both fed and fasted state of animals. Des-acyl ghrelin exerts inhibitory effects on the motility of antrum, but not on the motility of duodenum in the fasted state of ani-mals. Obestatin exerts inhibitory effects on the motility of antrum and duodenum in the fed state, but not in the fasted state of animals. NPY Y2 or Y4 receptors in the brain may mediate the action of ghrelin, CRF type 2 receptors in the brain mediate the action of des-acyl ghrelin, whereas CRF type 1 and type 2 receptors in the brain mediate the action of obestatin. Vagal affer-ent pathways might be involved in the action of ghre-lin, but not involved in the action of des-acyl ghrelin, whereas vagal afferent pathways might be partially involved in the action of obestatin.

  20. Distribution of Lymphocytes in the Mucosa Associated Lymphoid Tissues (MALT of Naturally Occurring Infectious Bursal Disease (IBD in Chicken

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    M. M. Uddin*, M. Z. I. Khan1, K. N. Islam, A. S. M. G. Kibria, G. N. Adhikary2, M. N. H. Parvez3, J. Basu, M. B. Uddin4 and M. M. Rahman5

    2010-04-01

    Full Text Available This study was aimed to investigate changes in the number and distribution of lymphocytes in the mucosa associated lymphoid tissues (MALT of digestive tract (proventriculus, duodenum, jejunum, ileum, cecum and cecal tonsils and respiratory system (lungs of chicken infected by Infectious Bursal Disease Virus (IBDV. Samples were divided into two groups; IBDV infected group (21, 24 and 30 days old and control group (non infected birds; 21 days old. Haematoxylin and eosin stained slides were prepared for microscopic studies to observe the distribution and the number of lymphocytes in the mucosa of the digestive tract and respiratory system. Lymphocytes were significantly (P<0.05 lower in proventriculus, duodenum, jejunum, ileum, cecum, cecal tonsils and lungs of IBDV infected chickens than the control. Moreover, the reduction in lymphocytes number was maximum in duodenum and cecal tonsils, while minimal in lungs. Depletion of lymphocyte was mainly in the lamina propria and the core of the villi and depletion increased with the advance of age of IBDV infected chicken. These results demonstrate that IBDV destroys the lymphocytes of the MALT and suppresses the immunity.

  1. Enzyme replacement therapy for pancreatic insufficiency: present and future

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    Fieker A

    2011-05-01

    Full Text Available Aaron Fieker1, Jessica Philpott1, Martine Armand21Division of Digestive Diseases, University of Oklahoma, OKC, OK, USA; 2INSERM, U476 "Nutrition Humaine et Lipides", Marseille, F-13385 France; Univ Méditerranée Aix-Marseille 2, Faculté de Médecine, IPHM-IFR 125, Marseille, F-13385 FranceAbstract: Pancreatic enzyme replacement therapy is currently the mainstay of treatment for nutrient malabsorption secondary to pancreatic insufficiency. This treatment is safe and has few side effects. Data demonstrate efficacy in reducing steatorrhea and fat malabsorption. Effective therapy has been limited by the ability to replicate the physiologic process of enzyme delivery to the appropriate site, in general the duodenum, at the appropriate time. The challenges include enzyme destruction in the stomach, lack of adequate mixing with the chyme in the duodenum, and failing to deliver and activate at the appropriate time. Treatment is begun when clinically significant malabsorption occurs resulting in steatorrhea and weight loss. Treatment failure is addressed in a sequential fashion. Current research is aimed at studying new enzymes and delivery systems to improve the efficiency of action in the duodenum along with developing better means to monitor therapy.Keywords: exocrine pancreatic insufficiency, chronic pancreatitis, cystic fibrosis, pancreatic enzyme replacement therapy, lipase, lipids

  2. War, traffic and iatrogenic injuries of D3 duodenal segment

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    Ignjatović Dragan

    2005-01-01

    Full Text Available Background. Injuries of the duodenum at the level of aortomesenteric clamp (segment D3 are with a high incidence of death due to the development of fistula and peritonitis. In three successfully managed cases, we applied the biliary surgery method. Case reports. All three cases were with the injuries of D3 duodenal segment. The first patient suffered from the blast perforation of duodenum at the level of the aortomesenteric clamp which occurred at the 7th day after the injury. The second patient suffered from the duodenal injury caused in a traffic accident. The third patient suffered from an iatrogenic injury at the beginning of D3 duodenal segment inflicted during ureterolithotomy. The described surgical procedure included basically the suture to narrow the site of the injury, then lateroterminal anastomosis with the Roux-en-Y jejunal flexure and, finally, the placement of a silicone prosthesis starting from the duodenum through the site of injury and the Roux-en-Y out. Octreotide and the total parenteral nutrition were administered to the patients postoperatively. Conclusion. The use of the releasing silicone prosthesis in all three patients provided the repair of the site of the injury with anastomosed Reux-en-Y jejunum.

  3. Dual-phase CT findings of groove pancreatitis

    Energy Technology Data Exchange (ETDEWEB)

    Zaheer, Atif, E-mail: azaheer1@jhmi.edu [The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins Medical Institutions, Baltimore, MD 21231 (United States); Pancreatitis Center, Division of Gastroenterology, Department of Medicine, Johns Hopkins Medical Institutions, Baltimore, MD 21231 (United States); Haider, Maera, E-mail: mhaider3@jhmi.edu [The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins Medical Institutions, Baltimore, MD 21231 (United States); Kawamoto, Satomi, E-mail: skawamo1@jhmi.edu [The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins Medical Institutions, Baltimore, MD 21231 (United States); Hruban, Ralph H., E-mail: rhruban1@jhmi.edu [Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, the Johns Hopkins University School of Medicine, Baltimore, MD 21231 (United States); Fishman, Elliot K., E-mail: efishma1@jhmi.edu [The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins Medical Institutions, Baltimore, MD 21231 (United States)

    2014-08-15

    Purpose: Groove pancreatitis is a rare focal form of chronic pancreatitis that occurs in the pancreaticoduodenal groove between the major and minor papillae, duodenum and pancreatic head. Radiologic appearance and clinical presentation can result in suspicion of malignancy rendering pancreaticoduodenectomy inevitable. This study reports dual phase CT findings in a series of 12 patients with pathology proven groove pancreatitis. Materials and methods: Retrospective review of preoperative CT findings in 12 patients with histologically proven groove pancreatitis after pancreaticoduodenectomy. Size, location, attenuation, presence of mass or cystic components in the pancreas, groove and duodenum, calcifications, duodenal stenosis and ductal changes were recorded. Clinical data, laboratory values, endoscopic ultrasonographic and histopathological findings were collected. Results: Soft tissue thickening in the groove was seen in all patients. Pancreatic head, groove and duodenum were all involved in 75% patients. A discrete lesion in the pancreatic head was seen in half of the patients, most of which appeared hypodense on both arterial and venous phases. Cystic changes in pancreatic head were seen in 75% patients. Duodenal involvement was seen in 92% patients including wall thickening and cyst formation. The main pancreatic duct was dilated in 7 patients, with an abrupt cut off in 3 and a smooth tapering stricture in 4. Five patients had evidence of chronic pancreatitis with parenchymal calcifications. Conclusion: Presence of mass or soft tissue thickening in the groove with cystic duodenal thickening is highly suggestive of groove pancreatitis. Recognizing common radiological features may help in diagnosis and reduce suspicion of malignancy.

  4. Endoscopic Removal of a Duodenal-Perforating Leg of Glasses with Dormia Basket

    Science.gov (United States)

    Wang, Li; Wen, Wei; Huang, Jiamiao; Hu, Weijie; Zhou, Renrong; Li, Xin; Wang, Xiaojiang

    2016-01-01

    Ingestion of foreign bodies is common in clinical practice. Most ingested foreign bodies will pass through the gastrointestinal (GI) tract without any problems. While GI tract injury due to the ingested foreign body such as a toothpick, a fishbone, a date pit, or a chicken bone, is common, duodenal perforation is rare. In this report, our experience with this rare entity is shared. We present a 38-year-old male patient with GI tract perforation in the bulbus of the duodenum due to a leg of glasses. The patient was admitted to our hospital with severe abdominal pain. Right upper quadrant tenderness was detected at physical examination, and leukocytosis on the laboratory test results. Plain X-ray and computerized tomography showed an ingested foreign body in the bulbus of the duodenum. A leg of glasses perforating the duodenum was removed with endoscopy. The patient was managed nonoperatively, and discharged without any complications on the eighth day after endoscopy. Endoscopic removal and nonoperative management may be feasible in carefully selected patients with duodenal-perforating foreign bodies. PMID:27920661

  5. Idiopathic Retroperitoneal Hematoma

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    Tomoyuki Abe

    2010-09-01

    Full Text Available A 34-year-old female presented with sudden onset of severe abdominal pain in a flank distribution. A large mass was palpable in the right upper quadrant on physical examination. Abdominal contrast-enhanced computed tomography showed a well-defined, right-sided, retroperitoneal cystic lesion located between the abdominal aorta and the inferior vena cava (IVC. The tumor size was 55 × 58 mm, and it compressed the gallbladder and the duodenum. Upper gastrointestinal radiography revealed a stricture of the second portion of the duodenum by the tumor. T2-weighted magnetic resonance imaging showed that the whole part was hyperintense with hypointense rims, but the inner was partially hypointense. Based on the radiological findings, the preoperative differential diagnosis included retroperitoneal teratoma, Schwannoma, abscess, and primary retroperitoneal tumor. On laparotomy, the tumor was located in the right retroperitoneal cavity. Kocher maneuver and medial visceral rotation, which consists of medial reflection of the upper part of right colon and duodenum by incising their lateral peritoneal attachments, were performed. Although a slight adhesion to the IVC was detected, the tumor was removed safely. Thin-section histopathology examination detected neither tumor tissues nor any tissues such as adrenal gland, ovarian tissue, or endometrial implants. The final pathological diagnosis was idiopathic retroperitoneal hematoma; the origin of the bleeding was unclear. The patient was discharged without any complication 5 days after the operation.

  6. Sulfapyridine appearance in plasma after salicylazosulfapyridine. Another simple measure of intestinal transit

    Energy Technology Data Exchange (ETDEWEB)

    Kellow, J.E.; Borody, T.J.; Phillips, S.F.; Haddad, A.C.; Brown, M.L.

    1986-08-01

    The appearance of sulfapyridine in plasma after oral administration of salicylazosulfapyridine (SASP) was evaluated as a method for defining arrival time in the cecum, an index of small bowel transit. After direct instillation of SASP and lactulose into the cecum, the appearances of their metabolites (sulfapyridine in plasma and hydrogen in breath) were rapid (1-10 min) and simultaneous. When a mixture of SASP and lactulose was taken by mouth, times of the respective signals varied among individuals from 40 to 180 min (n = 8) but were correlated within individuals. Salicylazosulfapyridine transit times from duodenum to cecum were also very similar to simultaneous measurements of transit by scintigraphic monitoring of technetium 99m. Timing of the sulfapyridine signal corresponded to the arrival of 5%-13% of technetium 99m DTPA in the cecum. Exemplifying the use of this new technique, simultaneous administration of lactulose into the stomach and SASP into the duodenum yielded consistently longer stomach-to-cecum than duodenum-to-cecum transits, attributable to the delay caused by gastric emptying. Therapeutic doses of morphine delayed small bowel transit of SASP. Transit of SASP offers a second marker technique for the cecal arrival of the head of a bolus; the approach may be useful as an inexpensive, noninvasive measurement of transit.

  7. Surgical management of endoscopically unresectable duodenal gangliocytic paraganglioma in a patient with partial upper gastrointestinal obstruction

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    David J Bertsch

    2010-11-01

    Full Text Available Context: Gangliocytic paragangliomas are unusual and often misunderstood tumors that occur almost exclusively in the second portion of the duodenum, although they have been described in other sites such as the urinary bladder, spermatic cord, prostate, urethra, uterus and scalp. We describe our experience with the surgical management of an endoscopically unresectable gangliocytic paraganglioma located in the third part of the duodenum causing a partial upper gastrointestinal obstruction. Case Report: A fifty-two-year-old male presented to the Geisinger clinic with a four-year history of postprandial projectile vomiting associated with epigastric discomfort. Computed tomography scan revealed an oval-shaped filling defect in the third part of the duodenum. Endoscopic ultrasonography showed a 22 x 16 x 35 mm submucosal mass that was not amenable to an endoscopic resection. Exploratory laparotomy revealed an absence of extraduodenal involvement. A long-stalked tumor was successfully excised and extruded through a longitudinal duodenotomy. The pathology report showed a gangliocytic paraganglioma with negative lymph nodes. Conclusions: In patients presenting with prolonged recurrent attacks of vomiting, diagnostic workup to exclude anatomic causes is mandatory. Gangliocytic paraganglioma must be considered in the differential diagnosis of an intraduodenal tumor.

  8. Unusual Upper Gastrointestinal Bleeding due to Late Metastasis from Renal Cell Carcinoma: A Case Report

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    Wen-Tsan Chang

    2004-03-01

    Full Text Available A case of recurrent massive upper gastrointestinal bleeding originating from metastatic renal cell carcinoma is reported. A 63-year-old woman underwent right nephrectomy 9 years previously and experienced no recurrence during follow-up. A gradually enlarging ulcerative tumor over the bulb of the duodenum and four subsequent episodes of massive bleeding from this tumor occurred between June 2001 and March 2002. The patient underwent surgery in April 2002 for intractable bleeding from the tumor. Renal cell carcinoma metastasis to the duodenum was confirmed from the surgical specimen. Upper gastrointestinal bleeding due to malignancy is very rare and the duodenum is the least frequently involved site. Furthermore, a solitary late renal cell carcinoma metastasis 9 years after a nephrectomy is extremely uncommon. This case suggests that life-long follow-up of renal cell carcinoma patients is necessary, owing to unpredictable behavior and the possibility of long disease-free intervals. In nephrectomized patients suffering from gastrointestinal bleeding, complete evaluation, especially endoscopic examination, is indicated. The possibility of late recurrent renal cell carcinoma metastasis to the gastrointestinal tract should be kept in mind, although it is rare. If the patient is fit for surgery, metastatectomy is the first choice of treatment.

  9. The use of dilute calogen[reg] as a fat density oral contrast medium in upper abdominal computed tomography, compared with the use of water and positive oral contrast media

    Energy Technology Data Exchange (ETDEWEB)

    Ramsay, Duncan W.; Markham, Derrian H.; Morgan, Bruno; Rodgers, Peter M.; Liddicoat, Amanda J

    2001-08-01

    AIM: Oral contrast media are commonly given prior to computed tomography (CT) examination of the upper abdomen. Although positive oral contrast media are normally used, there is increasing interest in using negative agents such as water and less commonly fat density products. The aim of this study was to compare a positive oral contrast medium, water, and a diluted emulsion of arachis oil (Calogen[reg], a fat density food supplement) for assessment of the upper abdomen. MATERIALS AND METHODS: Seventy-one patients referred for upper abdominal CT were randomized to receive either 500 ml water, 2% sodium diatrizoate or a dilute suspension of Calogen[reg]. The CT images were scored independently by three radiologists. Distension and anatomical identification was assessed for the stomach, duodenum and jejunum; with anatomical identification recorded for the pancreas, retroperitoneum, liver, gallbladder and spleen. RESULTS: Dilute Calogen[reg] produced a significant improvement (P < 0.01) in distension and anatomical visualization of the stomach and proximal duodenum. Only minimal differences were demonstrated between the three contrast media for visualization of more distal small bowel or identification of the other upper abdominal viscera. Significantly more artifacts were caused by positive contrast media than with the Calogen[reg] mixture. CONCLUSION: A dilute suspension of Calogen[reg] as an oral contrast medium is recommended when disease is suspected within the stomach or proximal duodenum. Ramsay, D.W. et al. (2001)

  10. Rat duodenal motility in vitro: Prokinetic effects of DL-homocysteine thiolactone and modulation of nitric oxide mediated inhibition

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    Stojanović Marija

    2013-01-01

    Full Text Available Homocysteine is a significant but modifiable risk factor for vascular diseases. As gastrointestinal smooth musculature is similar to blood vessel muscles, we investigated how elevated homocysteine levels affect nitric oxide-mediated neurotransmission in the gut. There is accumulated evidence that a dysfunction of NO neurons in the myenteric plexus may cause various diseases in the gastrointestinal tract such as achalasia, diabetic gastroparesis and infantile hypertrophic pyloric stenosis. In the present study, we aimed to assess the effects of homocysteine on NO-mediated responses in vitro, and to examine the effects of DL-homocysteine thiolactone on the spontaneous motility of rat duodenum and nitrergic neurotransmission. DL-homocysteine thiolactone concentration of 10 μmol/L leads to the immediate increase in tone, amplitude and frequency of spontaneous movements in isolated rat duodenum. L-NAME (30 μmol/L leads to an increase in basal tone, amplitude and frequency of spontaneous contractions. The relaxations induced by EFS were significantly reduced in duodenal segments incubated in DL-homocysteine thiolactone compared with the control group. EFS-induced relaxations were inhibited by L-NAME in both experimental and control groups. These results suggest that a high level of homocysteine causes an important impairment of non-adrenergic non-cholinergic innervation of the rat duodenum. [Projekat Ministarstva nauke Republike Srbije, br. 175043

  11. Groove Pancreatitis: A Case Report and Review of Literature

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    Vallath Balakrishnan

    2007-09-01

    Full Text Available Context Groove pancreatitis is a rare type of segmental pancreatitis characterized by fibrous scars of the anatomic space between the dorsocranial part of the head of the pancreas, the duodenum, and the common bile duct. Case report A 40-year-old man, with a past history of chronic alcohol consumption presented with epigastric pain radiating to the back and intermittent vomiting and a weight loss of 9 kg. A CT of the abdomen revealed swelling of the pancreatic head, a hypodense mass and duodenal wall thickening with luminal narrowing. Peripancreatic fluid and dense strands were also seen. Upper gastrointestinal endoscopy revealed an edematous, shiny, reddish raised mucosa having a polypoid appearance with narrowing of the second portion of the duodenum. Histological examination of the duodenal biopsy specimens showed preservation of the crypt-villus ratio, and the submucosa showed Brunner gland hyperplasia. These findings appeared consistent with the diagnosis of groove pancreatitis. Presently, the patient is on conservative medical management with analgesics, proton pump inhibitors and a pancreatic enzyme supplement. Conclusions Groove pancreatitis often masquerades as pancreatic head carcinoma. This condition should be kept in mind when making the differential diagnosis between pancreatic masses and duodenal stenosis. In all cases of focal pancreatitis involving the head or uncinate process of the pancreas with involvement of the adjacent duodenum, the possibility of groove pancreatitis should be considered.

  12. Effects of acute heat stress on gene expression of brain-gut neuropeptides in broiler chickens.

    Science.gov (United States)

    Lei, L; Hepeng, L; Xianlei, L; Hongchao, J; Hai, L; Sheikhahmadi, A; Yufeng, W; Zhigang, S

    2013-11-01

    Heat stress-induced reduction in feed intake is an annoyance of the poultry industry. Feed intake is regulated by complex mechanisms in which brain-gut neuropeptides are involved, but the changes in such neuropeptides in broiler chickens during heat exposure remain unclear. In this study, we investigated the effects of acute heat stress (35°C, 6 h, and 65% relative humidity) on the gene expression of appetite-regulating peptides in the hypothalamus and gastrointestinal tract of broiler chickens at 42 d of age. The hypothalamic mRNA levels of neuropeptide Y, agouti-related peptide, pro-opiomelanocortin, cocaine- and amphetamine-regulated transcript, corticotropin-releasing hormone, melanocortin 4 receptor, melanin-concentrating hormone, prepro-orexin, cholecystokinin (CCK), and ghrelin did not significantly change (P>0.05) in the heat-exposed broiler chickens. However, the mRNA levels of ghrelin in the glandular stomach, duodenum, and jejunum significantly increased and the mRNA level of CCK in the duodenum significantly decreased. The results indicate that acute heat stress had no effect on the gene expression of central appetite-regulating peptides under current experimental conditions; however, some gastrointestinal tract peptides (e.g., ghrelin and CCK) might play a role in the regulation of appetite in acute heat-exposed broiler chickens. Furthermore, ghrelin in the glandular stomach, duodenum, and jejunum might be the main regulative target of acute heat stress induced anorexia.

  13. Modified technique for combined liver-small bowel transplantation in pigs

    Institute of Scientific and Technical Information of China (English)

    Zhen-Yu Yin; Xiao-Dong Ni; Feng Jiang; Ning Li; You-Sheng Li; Jie-Shou Li

    2003-01-01

    AIM: As the conventional combined liver-small bowel transplantation is complicated with many postoperative complications, the aim of this study was to describe a modified technique for the combined liver-small bowel transplantation with preservation of the duodenum, partial head of pancreas and hepatic biliary system in pigs.METHODS: Composite liver/small bowel allotransplantations were undertaken in 30 long-white pigs. The graft included liver, about 3 to 4 m proximal jejunum, duodenum and partial pancreatic head. Vessels reconstructions included subhepatic vena cava-vena cava anastomosis, aorta-aorta anastomosis and portal-splenic vein anastomosis.RESULTS: Without immunosuppressive treatment, the median survival time of the animals was 6 days (2 to 12days), and about 76.9 % (20/26) of the animals survived for more than 4 days after operation.CONCLUSION: The modified technique is feasible and safe for the composite liver/small bowel transplantation with duodenum and pancreas preserved in pigs. And also this technique can simplify the operation and decrease possible postoperative complications.

  14. Secondary aortoduodenal fistula

    Institute of Scientific and Technical Information of China (English)

    Girolamo Geraci; Franco Pisello; Francesco Li Volsi; Tiziana Facella; Lina Platia; Giuseppe Modica; Carmelo Sciumè

    2008-01-01

    Aorto-duodenal fistulae (ADF) are the most frequent aorto-enteric fistulae (80%), presenting with upper gastrointestinal bleeding. We report the first case of a man with a secondary aorto-duodenal fistula presenting with a history of persistent occlusive syndrome. A 59-year old man who underwent an aortic-bi-femoral bypass 5 years ago, presented with dyspepsia and biliary vomiting. Computed tomography scan showed in the third duodenal segment the presence of inflammatory tissue with air bubbles between the duodenum and prosthesis, adherent to the duodenum. The patient was submitted to surgery, during which the prosthesis was detached from the duodenum, the intestine failed to close and a gastro-jejunal anastomosis was performed. The post-operative course was simple, secondary ADF was a complication (0.3%-2%) of aortic surgery. Mechanical erosion of the prosthetic material into the bowel was due to the lack of interposed retroperitoneal tissue or the excessive pulsation of redundantly placed grafts or septic procedures. The third or fourth duodenal segment was most frequently involved. Diagnosis of ADF was difficult. Surgical treatment is always recommended by explorative laparotomy. ADF must be suspected whenever a patient with aortic prosthesis has digestive bleeding or unexplained obstructive syndrome. Rarely the clinical picture of ADF is subtle presenting as an obstructive syndrome and in these cases the principal goal is to effectively relieve the mechanical bowel obstruction.

  15. Adenocarcinoma arising in a heterotopic pancreas (Heinrich type III: a case report

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    Egashira Yutaro

    2010-02-01

    Full Text Available Abstract Introduction Heterotopic pancreatic cancer in the duodenum is a very rare disease. Only twelve cases have been reported worldwide to date. We report a rare case of malignant transformation of heterotopic pancreas (Heinrich type III in the duodenum with long-term survival of the patient, and review the 12 cases in the literature. Case presentation A 75-year-old Japanese man was admitted to our hospital complaining of nausea and vomiting. Endoscopy and upper gastrointestinal contrast study showed marked duodenal stenosis. A pylorus-preserving pancreaticoduodenectomy was performed. Histopathological examination of the surgically resected specimen showed malignant transformation of heterotopic pancreas (Heinrich type III in the duodenum. The postoperative course was uneventful, and the patient was discharged from the hospital on postoperative day 30. He is well and shows no signs of recurrence at the time of writing, six years after the surgery. Conclusion Adenocarcinoma arising within the heterotopic pancreas appears to be rare. It is difficult to obtain a correct diagnosis preoperatively. The management of heterotopic pancreas depends on the presence or absence of symptoms. If the patient is asymptomatic or benign, conservative treatment with regular follow-up is recommended. When the patient is symptomatic or there is a suspicion of malignancy, surgical management with intra-operative frozen section diagnosis is indicated.

  16. Endoscopic Removal of a Duodenal-Perforating Leg of Glasses with Dormia Basket

    Directory of Open Access Journals (Sweden)

    Li Wang

    2016-11-01

    Full Text Available Ingestion of foreign bodies is common in clinical practice. Most ingested foreign bodies will pass through the gastrointestinal (GI tract without any problems. While GI tract injury due to the ingested foreign body such as a toothpick, a fishbone, a date pit, or a chicken bone, is common, duodenal perforation is rare. In this report, our experience with this rare entity is shared. We present a 38-year-old male patient with GI tract perforation in the bulbus of the duodenum due to a leg of glasses. The patient was admitted to our hospital with severe abdominal pain. Right upper quadrant tenderness was detected at physical examination, and leukocytosis on the laboratory test results. Plain X-ray and computerized tomography showed an ingested foreign body in the bulbus of the duodenum. A leg of glasses perforating the duodenum was removed with endoscopy. The patient was managed nonoperatively, and discharged without any complications on the eighth day after endoscopy. Endoscopic removal and nonoperative management may be feasible in carefully selected patients with duodenal-perforating foreign bodies.

  17. The use of dilute Calogen as a fat density oral contrast medium in upper abdominal computed tomography, compared with the use of water and positive oral contrast media.

    Science.gov (United States)

    Ramsay, D W; Markham, D H; Morgan, B; Rodgers, P M; Liddicoat, A J

    2001-08-01

    Oral contrast media are commonly given prior to computed tomography (CT) examination of the upper abdomen. Although positive oral contrast media are normally used, there is increasing interest in using negative agents such as water and less commonly fat density products. The aim of this study was to compare a positive oral contrast medium, water, and a diluted emulsion of arachis oil Calogen, a fat density food supplement) for assessment of the upper abdomen. Seventy-one patients referred for upper abdominal CT were randomized to receive either 500 ml water, 2% sodium diatrizoate or a dilute suspension of Calogen. The CT images were scored independently by three radiologists. Distension and anatomical identification was assessed for the stomach, duodenum and jejunum; with anatomical identification recorded for the pancreas, retroperitoneum, liver, gallbladder and spleen. Dilute Calogen produced a significant improvement (P < 0.01) in distension and anatomical visualization of the stomach and proximal duodenum. Only minimal differences were demonstrated between the three contrast media for visualization of more distal small bowel or identification of the other upper abdominal viscera. Significantly more artifacts were caused by positive contrast media than with the Calogen mixture. A dilute suspension of Calogen as an oral contrast medium is recommended when disease is suspected within the stomach or proximal duodenum.

  18. Dramatic regression and bleeding of a duodenal GIST during preoperative imatinib therapy: case report and review

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    Schwandner Thilo

    2010-06-01

    Full Text Available Abstract Background Gastrointestinal stromal tumors (GISTs are the most common mesenchymal tumors of the digestive tract. The majority of GISTs is located in the stomach. Only 3-5% of GISTs are located in the duodenum associated with an increased risk of gastrointestinal bleeding as primary manifestation. With response rates of up to 90%, but complications like bleeding due to tumor necrosis in 3%, imatinib mesylate dramatically altered the pre- and postoperative therapy for GIST patients. Case presentation A 58-year-old female patient presented with acute upper gastrointestinal bleeding 2 weeks after a giant GIST of the duodenum had been diagnosed. Neoadjuvant imatinib therapy had been initiated to achieve a tumor downsizing prior to surgery. During emergency laparotomy a partial duodenopancreatectomy was performed to achieve a complete resection of the mass. Histology revealed a high-malignancy GIST infiltrating the duodenal wall. Adjuvant imatinib therapy was initiated. At follow-up (19 months the patient is still alive and healthy. Conclusion Giant GISTs of the duodenum are rare and - in contrast to other localizations - harbour a higher risk of serious bleeding as primary manifestation. Tumor necrosis and tumor bleeding are rare but typical adverse effects of imatinib therapy especially during treatment of high-malignancy GIST. In GIST patients with increased risk of tumor bleeding neoadjuvant imatinib therapy should thoroughly be performed during hospitalization. In cases of duodenal GIST primary surgery should be considered as treatment alternative.

  19. Analysis of the proteases activity in 13 organs of the doll fish (Andrias davidianus) digestive system%中国大鲵消化系统13种器官的蛋白水解酶种类和活性分析

    Institute of Scientific and Technical Information of China (English)

    乔志刚; 辛泽华; 李吉学; 沈国民; 徐存拴

    2003-01-01

    Protease activity in 13 doll fish, Andrias davidianus, digestive organs: tongue, esophagus, cardia, stomach,pylorus, duodenum, jejunum, ileum, rectum, cloacal chamber, liver, pancreas, gall bladder were analysed with gelatine polyacrylamide gel-electrophoresis (G-PAGE). Compared to rats and rhesus monkeys (Macaca mullatta tcheliensis ),protease activity in the Andrias davidianus digestive system was more varied and stronger. The activity of acid proteases>that of the neutral proteases>that of the alkaline proteases. The kinds and activities of neutral and alkaline proteases in the Andrias davidianus digestive system wereare very different, while the kinds of acid proteases in them were very simi-lar, but their activities are different. Analysing the activities of proteases in these organs shows that protease activity in the duodenum > that in rectum > that in stomach > that in pancreas > that in liver. Experimental results suggest that the function of the rectum and liver in Andrias daviclianus may be as important as that of the duodenum and pancreas in food degradation.

  20. NetB-producing and beta2-producing Clostridium perfringens associated with subclinical necrotic enteritis in laying hens in the Netherlands.

    Science.gov (United States)

    Allaart, Janneke G; de Bruijn, Naomi D; van Asten, Alphons J A M; Fabri, Teun H F; Gröne, Andrea

    2012-12-01

    Since 2006 increasing numbers of laying hen flocks with decreased production have been reported in the Netherlands. At necropsy, birds from affected flocks showed multifocal areas of necrosis in the duodenum. Histologically the duodenum had moderate to marked villus atrophy and fusion with crypt hyperplasia and a mixed inflammatory infiltrate within the lamina propria underlying focal areas of degenerative epithelium. Multifocally, free within the intestinal lumen and associated with epithelial necrosis, were marked numbers of large rod-shaped bacteria. Anaerobic culturing and subsequent toxin typing revealed, in 19 out of 73 affected birds, the presence of Clostridium perfringens strains, either type A or type C harbouring the atypical allele of cpb2 and netB. Eighteen out of these 19 birds carried C. perfringens strains capable of producing beta2 toxin in vitro and all of these birds harboured C. perfringens strains capable of producing NetB toxin in vitro. In contrast, specific pathogen free (SPF) birds lacked gross or histological lesions in their duodenum, and C. perfringens type C was isolated from four out of 15 SPF birds tested. One of these isolates harboured the consensus three allele of cpb2 that produced beta2 toxin in vitro. None of the C. perfringens isolates originating from SPF birds harboured netB. These findings might indicate that the NetB toxin produced by C. perfringens is associated with subclinical necrotic enteritis in layers, whereas the involvement of beta2 toxin in subclinical necrotic enteritis, if any, might be variant dependent.

  1. Ghrelin family of peptides and gut motility.

    Science.gov (United States)

    Asakawa, Akihiro; Ataka, Koji; Fujino, Kazunori; Chen, Chih-Yen; Kato, Ikuo; Fujimiya, Mineko; Inui, Akio

    2011-04-01

    Acyl ghrelin, des-acyl ghrelin, and obestatin are three peptides isolated from the gastrointestinal tract and encoded by the same preproghrelin gene. Three ghrelin gene products participate in modulating appetite, adipogenesis, glucose metabolism, cell proliferation, immune, sleep, memory, anxiety, cognition, and stress. We have investigated the effects of ghrelin family of peptides on fed and fasted motor activities in the stomach and duodenum of freely moving conscious rats by manometric method. Intracerebroventricular (ICV) and intravenous (IV) administration of acyl ghrelin induced fasted motor activity in the duodenum in fed rats. ICV and IV administration of des-acyl ghrelin disrupted fasted motor activity in the antrum. Changes in gastric motility induced by IV administration of des-acyl ghrelin were antagonized by ICV administration of a corticotropin-releasing factor (CRF) 2 receptor antagonist. IV administration of obestatin decreased the percentage motor index in the antrum and prolonged the time taken to return to fasted motility in the duodenum in fed rats. ICV administration of CRF 1 and 2 receptor antagonists prevented the effects of obestatin on gastroduodenal motility. Ghrelin gene products regulate feeding-associated gastroduodenal motility. Stomach may regulate various functions including gastrointestinal motility via acyl ghrelin, des-acyl ghrelin and obestatin as an endocrine organ. Increasing knowledge of the effects of ghrelin family of peptides on gastrointestinal motility could lead to innovative new therapies for functional gastrointestinal disorders.

  2. Ghrelin, Des-Acyl Ghrelin, and Obestatin: Regulatory Roles on the Gastrointestinal Motility

    Directory of Open Access Journals (Sweden)

    Mineko Fujimiya

    2010-01-01

    Full Text Available Ghrelin, des-acyl ghrelin, and obestatin are derived from a common prohormone, preproghrelin by posttranslational processing, originating from endocrine cells in the stomach. To examine the regulatory roles of these peptides, we applied the manometric measurement of gastrointestinal motility in freely moving conscious rat or mouse model. Ghrelin exerts stimulatory effects on the motility of antrum and duodenum in both fed and fasted state of animals. Des-acyl ghrelin exerts inhibitory effects on the motility of antrum but not on the motility of duodenum in the fasted state of animals. Obestatin exerts inhibitory effects on the motility of antrum and duodenum in the fed state but not in the fasted state of animals. NPY Y2 and Y4 receptors in the brain may mediate the action of ghrelin, CRF type 2 receptor in the brain may mediate the action of des-acyl ghrelin, whereas CRF type 1 and type 2 receptors in the brain may mediate the action of obestatin. Vagal afferent pathways might be involved in the action of ghrelin, but not involved in the action of des-acyl ghrelin, whereas vagal afferent pathways might be partially involved in the action of obestatin.

  3. Melanocortin-4 receptor expression in a vago-vagal circuitry involved in postprandial functions.

    Science.gov (United States)

    Gautron, Laurent; Lee, Charlotte; Funahashi, Hisayuki; Friedman, Jeffrey; Lee, Syann; Elmquist, Joel

    2010-01-01

    Vagal afferents regulate energy balance by providing a link between the brain and postprandial signals originating from the gut. In the current study, we investigated melanocortin-4 receptor (MC4R) expression in the nodose ganglion, where the cell bodies of vagal sensory afferents reside. By using a line of mice expressing green fluorescent protein (GFP) under the control of the MC4R promoter, we found GFP expression in approximately one-third of nodose ganglion neurons. By using immunohistochemistry combined with in situ hybridization, we also demonstrated that approximately 20% of GFP-positive neurons coexpressed cholecystokinin receptor A. In addition, we found that the GFP is transported to peripheral tissues by both vagal sensory afferents and motor efferents, which allowed us to assess the sites innervated by MC4R-GFP neurons. GFP-positive efferents that co-expressed choline acetyltransferase specifically terminated in the hepatic artery and the myenteric plexus of the stomach and duodenum. In contrast, GFP-positive afferents that did not express cholinergic or sympathetic markers terminated in the submucosal plexus and mucosa of the duodenum. Retrograde tracing experiments confirmed the innervation of the duodenum by GFP-positive neurons located in the nodose ganglion. Our findings support the hypothesis that MC4R signaling in vagal afferents may modulate the activity of fibers sensitive to satiety signals such as cholecystokinin, and that MC4R signaling in vagal efferents may contribute to the control of the liver and gastrointestinal tract.

  4. Duodenal ileus caused by a calf feeding nipple in a cow

    Directory of Open Access Journals (Sweden)

    Gerspach Christian

    2011-01-01

    Full Text Available Abstract Background The aim of this report was to describe duodenal obstruction caused by a rubber foreign body in a cow. Case Presentation The clinical, biochemical and ultrasonographic findings in a five-year-old Swiss Braunvieh cow with duodenal ileus caused by a calf feeding nipple are described. The main clinical signs were anorexia, ruminal tympany, decreased faecal output and abomasal reflux syndrome. Ultrasonographic examination revealed reticular hyperactivity and a dilated duodenum. A diagnosis of duodenal ileus was made and the cow underwent right-flank laparotomy, which revealed a dilation of the cranial part of the duodenum because of obstruction by a pliable foreign body. This was identified via enterotomy as a calf feeding nipple. The cow was healthy at the time of discharge four days after surgery and went on to complete a successful lactation. Conclusions To our knowledge, this is the first description of duodenal obstruction by a calf feeding nipple. This is an interesting case, which broadens the spectrum of the causes of duodenal ileus, which is usually caused by obstruction of the duodenum by a phytobezoar.

  5. Influence of delayed placement and dietary lysine levels on small intestine morphometrics and performance of broilers

    Directory of Open Access Journals (Sweden)

    JRG Franco

    2006-12-01

    Full Text Available This experiment studied the influence of delayed placement (HI and digestible lysine level (DL on the morphometrics of the intestinal mucosa and on the performance of broilers. A total number of 1,705 Cobb 500 male chicks were used in a completely randomized experimental design in a factorial arrangement with four HI (12, 24, 36 and 48h, and two DL level in the starter diet (1.143 and 1.267%, with four replicates and 55 birds per experimental unit. The amino acids methionine-cystine, threonine, and tryptophan were balanced according to the ideal protein (IP concept. Small intestine morphometrics was evaluated using histology slides of the duodenum and jejunum. There was no interaction between HI and DL levels for any of the studied parameters. The 1.143% level of DL promoted better performance results at 21 and 42 days of age, as well as higher duodenum and jejunum crypt depth, and duodenum villi height at 21 days of age. HI negatively influenced the morphometrics of the small intestine during the starter phase, and the performance of broilers up to 42 days of age. There was no effect of the treatments on yolk sac utilization or abdominal fat percentage. It was concluded that the use of 1.143% DL and HI of 12 hours promoted better development of the small intestine mucosa up to 21 days of age, and broiler performance at market age.

  6. Effects of different levels of protein supplements in the diet of early-weaned yaks on growth performance, intestinal development, and immune response to tuberculosis

    Directory of Open Access Journals (Sweden)

    Haibo Zhang

    2015-06-01

    Full Text Available This study was conducted to determine the effects of different levels of crude protein (CP supplements to the diet of early-weaned yaks on their growth performance, intestinal development, and immune response. Forty 3-month-old weaned yaks were selected and assigned to four dietary groups (Control, 17, 19 and 21% CP. Dietary CP supplements had a significant effect on average daily gain (ADG, crypt depth (CD (duodenum, jejunum and ileum, villous height (VH (duodenum, jejunum and ileum and CD/VH (jejunum and ileum. Average daily gain, CD (duodenum, jejunum and ileum and VH (ileum showed quadratic increases as the dietary CP increased, whereas CD/VH (jejunum and ileum ratios showed quadratic decreases. Blood urea nitrogen (BUN, glucose (GLU, immunoglobulin G (IgG, IgM, interleukin-1 (IL-1, IL-2, tumor necrosis factor (TNF-α, and interferon (IFN-γ concentrations increased significantly, whereas albumin (ALB, alanine aminotransferase (ALT and aspartate aminotransferase (AST decreased significantly with dietary CP supplements. Dietary CP supplements significantly increased the concentrations of IL-6, TNF-α, IFN-γ and the nuclear factor of activated T cell transcription factor (NFAT for gene expression. As the dietary CP supplements increased, IL-6, IFN-γ and NF-AT gene expression showed quadratic increases. These results showed that the appropriate dietary CP supplementation improved the growth performance and intestinal development of earlyweaned yaks and thus that the CP supplements were beneficial and enhanced the humoral immunity response of yaks.

  7. Surgical Strategy in Bouveret's Syndrome. A Case Report

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    Dénes Márton István

    2016-06-01

    Full Text Available Bouveret's syndrome is a high mechanical obstruction due to impaction of a gallstone into the duodenum, through a cholecystoduodenal fistula. It belongs to a larger group of gallstone ileus, a disease which occurs after developing a fistula between the gallbladder and the gastrointestinal tract. This is a rare complication of gallstones but because it appears in elderly people, it has a high morbidity and mortality. Patients have various symptoms and the treatment is individualized. We present here a case of a 67 years old patient, admitted in emergency, presenting symptoms of high bowel obstruction, with onset 5 days before admission. Abdominal ultrasound reveals a 5 cm stone that seems to be in the gallbladder, gastric stasis and at gastroscopy appears a foreign body impacted in the duodenum. After a short preparation the patient underwent surgery. We found a dilated stomach and a large cholecystoduodenal fistula with an impacted gallstone in the duodenum. We performed one stage surgery: cholecystectomy, extraction of the stone and suturing of the fistula. We reestablished the continuity of the intestinal tract. We performed also an ileostomy for feeding the patient and protecting the anastomoses. The postoperative evolution was favorable.

  8. Dose-Volume Analysis of Predictors for Gastrointestinal Toxicity After Concurrent Full-Dose Gemcitabine and Radiotherapy for Locally Advanced Pancreatic Adenocarcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Huang Jiayi [Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, MI (United States); Robertson, John M., E-mail: jrobertson@beaumont.edu [Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, MI (United States); Ye Hong [Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, MI (United States); Margolis, Jeffrey; Nadeau, Laura [Division of Medical Oncology, William Beaumont Hospital, Royal Oak, MI (United States); Yan Di [Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, MI (United States)

    2012-07-15

    Purpose: To identify dosimetric predictors for the development of gastrointestinal (GI) toxicity in patients with locally advanced pancreatic adenocarcinoma (LAPC) treated with concurrent full-dose gemcitabine and radiotherapy (GemRT). Methods and Materials: From June 2002 to June 2009, 46 LAPC patients treated with definitive GemRT were retrospectively analyzed. The stomach and duodenum were retrospectively contoured separately to determine their dose-volume histogram (DVH) parameters. GI toxicity was defined as Grade 3 or higher GI toxicity. The follow-up time was calculated from the start of RT to the date of death or last contact. Univariate analysis (UVA) and multivariate analysis (MVA) using Kaplan-Meier and Cox regression models were performed to identify risk factors associated with GI toxicity. The receiver operating characteristic curve and the area under the receiver operating characteristic curve (AUC) were used to determine the best DVH parameter to predict for GI toxicity. Results: Of the patients, 28 (61%) received concurrent gemcitabine alone, and 18 (39%) had concurrent gemcitabine with daily erlotinib. On UVA, only the V{sub 20Gy} to V{sub 35Gy} of duodenum were significantly associated with GI toxicity (all p {<=} 0.05). On MVA, the V{sub 25Gy} of duodenum and the use of erlotinib were independent risk factors for GI toxicity (p = 0.006 and 0.02, respectively). For the entire cohort, the V{sub 25Gy} of duodenum is the best predictor for GI toxicity (AUC = 0.717), and the 12-month GI toxicity rate was 8% vs. 48% for V{sub 25Gy} {<=} 45% and V{sub 25Gy} > 45%, respectively (p = 0.03). However, excluding the erlotinib group, the V{sub 35Gy} is the best predictor (AUC = 0.725), and the 12-month GI toxicity rate was 0% vs. 41% for V{sub 35Gy} {<=} 20% and V{sub 35Gy} > 20%, respectively (p = 0.04). Conclusions: DVH parameters of duodenum may predict Grade 3 GI toxicity after GemRT for LAPC. Concurrent use of erlotinib during GemRT may increase GI

  9. SURVIVAL OF MICROORGANISMS FROM MODERN PROBIOTICS IN MODEL CONDITIONS OF THE INTESTINE

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    Kabluchko TV

    2017-03-01

    Full Text Available Introduction. The staye of intestinal microflora affects the work of the whole organism. When composition of normal ibtestine microflora changes, its restoration is required. In our days a wide variety of probiotic drugs are available on the market which can be used to solve this problem. Most bacteria having probiotic properties represent the families Lactobacillus and Bifidobacterium, which have poor resistance to acidic content of the stomach and toxic effects of bile salts. Various studies have clearly shown that in a person with normal acidic and bile secretion, the lactobacilli and bifidobacteria are not detected after the passage through the duodenum, i.e., they perish before reaching the small intestines. In this study we compared the survival of different microorganisms which are contained in 9 probiotic drugs in a model of gastric and intestinal environments. Material and methods. In the laboratory of SI: “Mechnikov Institute Microbiology and Immunology, National Ukrainian Academy Medical Sciences" the in vitro experiments have been evaluated to test the ability of different probiotic bacteria which were contained in 9 probiotic drugs to survive the impact of the model environment of the stomach and duodenum. Bacillus coagulans persistence was evaluated under impact of simulated environment of the stomach and duodenum, it also was assessed by the quantity of CFU by incubation on culture medium. The following were studied: Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus bulgaricus, Bifidobacterium bifidum, Bifidobacterium longum , Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium animalis subsp. Lactis BB-12, Saccharomyces boulardii, Bacillus coagulans, Bacillus clausii, Enterococcus faecium. Microorganisms were incubated for 3 hours in a model environment of the stomach (pepsin 3 g / l, hydrochloric acid of 160 mmol / l, pH 2

  10. Morphological Studies of Digestive Tract and Its Argyrophil Cells in Sunken Ear Frog%凹耳蛙消化道组织学和嗜银细胞形态观察

    Institute of Scientific and Technical Information of China (English)

    马雪泷; 唐鑫生; 吴仁红; 马亚军; 张蕾

    2012-01-01

    为了揭示凹耳蛙( Odorrana tormota)消化道的基本特征,运用石蜡切片法和龙桂开银浸法对凹耳蛙消化道组织学结构及嗜银细胞的形态与分布密度进行了观察.结果显示:①凹耳蛙的胃壁具明显的纵行皱襞和胃小凹,胃腺发达,小肠可分为十二指肠和回肠,杯状细胞分散在十二指肠上皮细胞之间,十二指肠中未见十二指肠腺分布.②凹耳蛙嗜银细胞见于消化道全长,呈毛笔头样、锥体形、梭形、椭圆形和长条形等;幽门腺上皮和十二指肠绒毛上皮中的嗜银细胞具指向腺泡腔或肠腔的突起,提示其可能具有腔分泌的功能.嗜银细胞的分布密度胃幽门部最高,十二指肠和胃体其次,食道最低.据此认为胃既是凹耳蛙的主要消化器官,也是消化道中主要的内分泌器官;十二指肠是凹耳蛙消化道中的主要吸收部位,同时也具有内分泌功能;消化道嗜银细胞具有内分泌的功能,还可能具有腔分泌的功能.%To reveal the basic characteristics of the digestive tract of Sunken Ear Frog( Odonana tormota) , paraffin section and Longguikai's silver staining were used to observe histological characteristics and distribution density of argyrophil cells in the digestive tract. Longitudinal plica and gastric pits were obviously observed on the gastric wall and glands were well developed. The small intestine could be divided into duodenum and ileum, with goblet cells scattered among the duodenum epithelial cells,while no glands were found in the duodenum. Argyrophil cells in various shapes, such as brush-headed, conical, spindle-like, elliptical and rectangular, were observed along the whole digestive tract, and these cells in gland epithelium of pilori and villus epithelium of duodenum had apophysis pointing to the acinar lumen or intestine, indicating the possible luminal secretion function of these cells. Pylorica part of suomach had the highest density of argyrophil cells

  11. Elenoside increases intestinal motility

    Institute of Scientific and Technical Information of China (English)

    E Navarro; SJ Alonso; R Navarro; J Trujillo; E Jorge

    2006-01-01

    AIM: To study the effects of elenoside, an arylnaphthalene lignan from Justicia hyssopifolia, on gastrointestinal motility in vivo and in vitro in rats.METHODS: Routine in vivo experimental assessments were catharsis index, water percentage of boluses,intestinal transit, and codeine antagonism. The groups included were vehicle control (propylene glycol-ethanolplant oil-tween 80), elenoside (i.p. 25 and 50 mg/kg),cisapride (i.p. 10 mg/kg), and codeine phosphate (intragastric route, 50 mg/kg). In vitro approaches used isolated rat intestinal tissues (duodenum, jejunum, and ileum). The effects of elenoside at concentrations of 3.2× 10-4, 6.4 × 10-4 and 1.2 × 10-3 mol/L, and cisapride at 10-6 mol/L were investigated.RESULTS: Elenoside in vivo produced an increase in the catharsis index and water percentage of boluses and in the percentage of distance traveled by a suspension of activated charcoal. Codeine phosphate antagonized the effect of 25 mg/kg of elenoside. In vitro, elenoside in duodenum, jejunum and ileum produced an initial decrease in the contraction force followed by an increase.Elenoside resulted in decreased intestinal frequency in duodenum, jejunum, and ileum. The in vitro and in vivo effects of elenoside were similar to those produced by cisapride.CONCLUSION: Elenoside is a lignan with an action similar to that of purgative and prokinetics drugs.Elenoside, could be an alternative to cisapride in treatment of gastrointestinal diseases as well as a preventive therapy for the undesirable gastrointestinal effects produced by opioids used for mild to moderate pain.

  12. Molecular cloning of motilin and mechanism of motilin-induced gastrointestinal motility in Japanese quail.

    Science.gov (United States)

    Apu, Auvijit Saha; Mondal, Anupom; Kitazawa, Takio; Takemi, Shota; Sakai, Takafumi; Sakata, Ichiro

    2016-07-01

    Motilin, a peptide hormone produced in the upper intestinal mucosa, plays an important role in the regulation of gastrointestinal (GI) motility. In the present study, we first determined the cDNA and amino acid sequences of motilin in the Japanese quail and studied the distribution of motilin-producing cells in the gastrointestinal tract. We also examined the motilin-induced contractile properties of quail GI tracts using an in vitro organ bath, and then elucidated the mechanisms of motilin-induced contraction in the proventriculus and duodenum of the quail. Mature quail motilin was composed of 22 amino acid residues, which showed high homology with chicken (95.4%), human (72.7%), and dog (72.7%) motilin. Immunohistochemical analysis showed that motilin-immunopositive cells were present in the mucosal layer of the duodenum (23.4±4.6cells/mm(2)), jejunum (15.2±0.8cells/mm(2)), and ileum (2.5±0.7cells/mm(2)), but were not observed in the crop, proventriculus, and colon. In the organ bath study, chicken motilin induced dose-dependent contraction in the proventriculus and small intestine. On the other hand, chicken ghrelin had no effect on contraction in the GI tract. Motilin-induced contraction in the duodenum was not inhibited by atropine, hexamethonium, ritanserin, ondansetron, or tetrodotoxin. However, motilin-induced contractions in the proventriculus were significantly inhibited by atropine and tetrodotoxin. These results suggest that motilin is the major stimulant of GI contraction in quail, as it is in mammals and the site of action of motilin is different between small intestine and proventriculus.

  13. Clinicoradiological appraisal of ′paraduodenal pancreatitis′: Pancreatitis outside the pancreas!

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    Ankur Arora

    2015-01-01

    Full Text Available Purpose: Paraduodenal pancreatitis (PP is a unique form of focal chronic pancreatitis that selectively involves the duodenum and aberrant pancreatic tissue located near the minor papilla (beyond the pancreas proper. The pseudotumoral nature of the disease often generates considerable clinical quandary and patient apprehension, and therefore merits a better understanding. The present study appraises the clinicoradiological manifestations of PP in 33 patients. Materials and Methods: Clinical, laboratory, and radiological manifestations of 33 patients of PP treated in gastroenterology/hepatology and hepato-pancreatico-biliary surgery units during June 2010-August 2014 were retrospectively reviewed. Results: All patients were young to middle-aged men (100% with history of alcohol abuse (93.9% and/or smoking (42.4%, who presented either with acute or gradually worsening abdominal pain (90.9%. Pancreatic enzymes and serum tumor markers remained normal or were mildly/transiently elevated. Cystic variant was detected in 57.6% (solid in 42.4%; the disease remained confined to the groove/duodenum (pure form in 45.4%. Medial duodenal wall thickening with increased enhancement was seen in 87.87 and 81.81%, respectively, and duodenal/paraduodenal cysts were seen in 78.78%. Pancreatic calcifications and biliary stricture were seen 27.3% patients. Peripancreatic arteries were neither infiltrated nor encased. Conclusion: PP has a discrete predilection for middle-aged men with history of longstanding alcohol abuse and/or smoking. Distinguishing imaging findings include thickening of the pancreatic side of duodenum exhibiting increased enhancement with intramural/paraduodenal cysts. This may be accompanied by plate-like scar tissue in the groove region, which may simulate groove pancreatic carcinoma. However, as opposed to carcinoma, the peripancreatic arteries are neither infiltrated nor encased, rather are medially displaced.

  14. Comparison of solcoseryl and epidermal growth factors (EGF) in healing of chronic gastroduodenal ulcerations and mucosal growth in rats.

    Science.gov (United States)

    Konturek, S J; Brzozowski, T; Dembinski, A; Warzecha, A; Drozdowicz, D

    1988-02-01

    Solcoseryl, a deproteinized extract of calf blood, and EGF, produced by salivary glands, have been shown to enhance the healing of peptic ulcerations, but the mechanism of this effect is unknown. Since both solcoseryl and EGF have been reported to stimulate cell proliferation, we designed the study to compare the ulcer healing and growth promoting actions of these two agents in the same animals. Gastric and duodenal ulcerations were produced by serosal application of 100% acetic acid on an area of 13.8 mm2 of gastric and duodenal wall, respectively. In the control animals, 7 days after ulcer induction, the mean ulcer area was reduced to 7.1 +/- 1.2 mm2 in the stomach, and to 6.1 +/- 0.8 mm2 in the duodenum. After 14 days all ulcers were healed, both in the stomach and duodenum. Oral administration of solcoseryl (10 ml/kg-day) or EGF (30 micrograms/kg-day) for 7 days after ulcer induction resulted in a significant reduction in the ulcer area in the stomach, and to a greater extent in the duodenum. This enhancement of ulcer healing by solcoseryl was accompanied by a significant increase in the weight of the duodenal mucosa and the total contents of DNA and RNA after 7 days of treatment, and in the weight and nucleic acid contents in both the gastric and duodenal mucosa after 14 days of treatment. EGF also increased the weights and the nucleic acid contents in gastric and duodenal mucosa, but this was significant only after 14 days of treatment.(ABSTRACT TRUNCATED AT 250 WORDS)

  15. Rye affects bacterial translocation, intestinal viscosity, microbiota composition and bone mineralization in Turkey poults.

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    Guillermo Tellez

    Full Text Available Previously, we have reported that rye significantly increased both viscosity and Clostridium perfringens proliferation when compared with corn in an in vitro digestive model. Two independent trials were conducted to evaluate the effect of rye as a source of energy on bacterial translocation, intestinal viscosity, gut microbiota composition, and bone mineralization, when compared with corn in turkey poults. In each experiment, day-of-hatch, turkey poults were randomly assigned to either a corn or a rye diet (n = 0 /group. At 10 d of age, in both experiments, 12 birds/group were given an oral gavage dose of fluorescein isothiocyanate dextran (FITC-d. After 2.5 h of oral gavage, blood and liver samples were collected to evaluate the passage of FITC-d and bacterial translocation (BT respectively. Duodenum, ileum and cecum gut sections were collected to evaluate intestinal viscosity and to enumerate gut microbiota. Tibias were collected for observation of bone parameters. Broilers fed with a rye diet showed increased (p<0.05 intestinal viscosity, BT, and serum FITC-d. Bacterial enumeration revealed that turkey poults fed with rye had increased the number of total lactic acid bacteria (LAB in all three sections of the gastrointestinal tract evaluated when compared to turkey poults fed with corn. Turkey poults fed with rye also had significantly higher coliforms in duodenum and ileum but not in the ceca, whereas the total number of anaerobes increased only in duodenum. A significant reduction in bone strength and bone mineralization was observed in turkey poults fed with rye when compared with corn fed turkey poults. In conclusion, rye evoked mucosal damage in turkey poults that increased intestinal viscosity, increased leakage through the intestinal tract, and altered the microbiota composition and bone mineralization. Studies to evaluate dietary inclusion of selected Direct-Fed Microbial (DFM candidates that produce exogenous enzymes in rye fed

  16. Rye affects bacterial translocation, intestinal viscosity, microbiota composition and bone mineralization in Turkey poults.

    Science.gov (United States)

    Tellez, Guillermo; Latorre, Juan D; Kuttappan, Vivek A; Hargis, Billy M; Hernandez-Velasco, Xochitl

    2015-01-01

    Previously, we have reported that rye significantly increased both viscosity and Clostridium perfringens proliferation when compared with corn in an in vitro digestive model. Two independent trials were conducted to evaluate the effect of rye as a source of energy on bacterial translocation, intestinal viscosity, gut microbiota composition, and bone mineralization, when compared with corn in turkey poults. In each experiment, day-of-hatch, turkey poults were randomly assigned to either a corn or a rye diet (n = 0 /group). At 10 d of age, in both experiments, 12 birds/group were given an oral gavage dose of fluorescein isothiocyanate dextran (FITC-d). After 2.5 h of oral gavage, blood and liver samples were collected to evaluate the passage of FITC-d and bacterial translocation (BT) respectively. Duodenum, ileum and cecum gut sections were collected to evaluate intestinal viscosity and to enumerate gut microbiota. Tibias were collected for observation of bone parameters. Broilers fed with a rye diet showed increased (pBacterial enumeration revealed that turkey poults fed with rye had increased the number of total lactic acid bacteria (LAB) in all three sections of the gastrointestinal tract evaluated when compared to turkey poults fed with corn. Turkey poults fed with rye also had significantly higher coliforms in duodenum and ileum but not in the ceca, whereas the total number of anaerobes increased only in duodenum. A significant reduction in bone strength and bone mineralization was observed in turkey poults fed with rye when compared with corn fed turkey poults. In conclusion, rye evoked mucosal damage in turkey poults that increased intestinal viscosity, increased leakage through the intestinal tract, and altered the microbiota composition and bone mineralization. Studies to evaluate dietary inclusion of selected Direct-Fed Microbial (DFM) candidates that produce exogenous enzymes in rye fed turkey poults are currently being evaluated.

  17. Risk Factors for Gallstone Formation after Surgery for Gastric Cancer.

    Science.gov (United States)

    Park, Dong Jin; Kim, Ki Hyun; Park, Young Suk; Ahn, Sang-Hoon; Park, Do Joong; Kim, Hyung-Ho

    2016-06-01

    The incidence of gallstones after gastrectomy for gastric cancer is higher than in the general population. However, the causes and mechanisms of post-gastrectomy gallstones are unclear. The aim of this study was to evaluate the incidence of gallstone formation and the risk factors for their development after gastrectomy for gastric cancer. Of 1,744 gastric cancer patients who underwent gastrectomy at Seoul National University Bundang Hospital between January 2010 and December 2012, 1,284 were included in this study and retrospectively reviewed. Patients' age, sex, body mass index (BMI), tumor location, stage, type of gastrectomy, type of reconstruction, and extent of node dissection were evaluated. The incidence of gallstones after gastrectomy for gastric cancer was significantly higher in men than in women (P=0.019). Exclusion of the duodenum during reconstruction was associated with a significantly higher incidence of gallstones (P=0.003). Overweight and obese patients with BMI ≥23 kg/m(2) had significantly higher incidence of gallstones than those with a lower BMI (P=0.006). Multivariate analysis showed that obesity (hazard ratio, HR=1.614; 95% confidence interval, CI: 1.135~2.296; P=0.008), male sex (HR=1.515, 95% CI: 1.029~2.231, P=0.033), and exclusion of the duodenum (HR=1.648, 95% CI: 1.192~2.280, P=0.003) were significant, independent risk factors for gallstones after gastrectomy. The cumulative incidence of gallstones for 5 years after gastrectomy was 15.3%. Male sex, obesity, and exclusion of the duodenum were risk factors for gallstone formation after gastrectomy. Careful surveillance will be required for these patient groups after gastrectomy.

  18. Microbial community mapping in intestinal tract of broiler chicken.

    Science.gov (United States)

    Xiao, Yingping; Xiang, Yun; Zhou, Weidong; Chen, Jinggang; Li, Kaifeng; Yang, Hua

    2016-10-06

    Domestic chickens are valuable sources of protein associated with producing meat and eggs for humans. The gastrointestinal tract (GIT) houses a large microbial community, and these microbiota play an important role in growth and health of chickens, contributing to the enhancement of nutrient absorption and improvement of the birds' immune systems. To improve our understanding of the chicken intestinal microbial composition, microbiota inhabiting 5 different intestinal locations (duodenum, jejunum, ileum, cecum, and colon) of 42-day-old broiler chickens were detected based on 16S rRNA gene sequence analysis. As a result, 1,502,554 sequences were clustered into 796 operational taxonomic units (OTUs) at the 97% sequence similarity value and identified into 15 phyla and 288 genera. Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Cyanobacteria were the major microbial groups and Firmicutes was the dominant phylum in duodenum, jejunum, ileum and colon accounting for > 60% of sequences, while Bacteroidetes was the dominant phylum in cecum (>50% of sequences), but little in the other four gut sections. At the genus level, the major microbial genera across all gut sections were Lactobacillus, Enterococcus, Bacteroides, and Corynebacterium. Lactobacillus was the predominant genus in duodenum, jejunum, and ileum (>35%), but was rarely present in cecum, and Bacteroides was the most dominant group in cecum (about 40%), but rarely present in the other 4 intestinal sections. Differences of microbial composition between the 5 intestinal locations might be a cause and consequence of gut functional differences and may also reflect host selection mediated by innate or adaptive immune responses. All these results could offer some information for the future study on the relationship between intestinal microbiota and broiler chicken growth performance as well as health.

  19. Blueberry juice used per os in upper abdominal MR imaging: composition and initial clinical data

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    Karantanas, A.H. [Department of CT-MRI, Larissa General Hospital (Greece); Papanikolaou, N.; Gourtsoyiannis, N. [Medical School, University of Crete, Heraklion (Greece); Kalef-Ezra, J. [Department of Medical Physics, Medical School, University of Ioannina (Greece); Challa, A. [Medical School, University of Ioannina (Greece)

    2000-06-01

    The aim of this study was to evaluate the use of a commercially available blueberry juice (BJ) both as a positive and negative oral contrast agent and to present the exact contents of paramagnetic ions. The concentration of Mn and Fe were determined in tinned myrtilles in syrup (atomic absorption). Nine healthy volunteers and 12 patients (age range 20-65 years) were examined using a 1-T MR scanner before and after per os administration of 430 ml of BJ. A qualitative analysis of signal alterations in the stomach, duodenum, and proximal small intestine was performed. In addition, a quantitative analysis was assessed in terms of signal-to-noise ratio calculation. The mean concentration (x {+-} SD) of the ions found in the content of the three cans were 3.3 {+-} 0.4 {mu}g/g for iron and 20.6 {+-} 2.6 {mu}g/g for manganese. Based on the qualitative evaluation, signal alteration on T1-weighted images after administration of BJ was statistically significant in the stomach and duodenum, but not in the proximal small bowel. Signal alteration on T2-weighted images was not statistically significant in any part of the gastrointestinal tract. The quantitative analysis of the T1- and T2 shortening showed that BJ is efficient with only T1-weighted sequences, and this applied to the stomach, duodenum, and proximal small bowel. Blueberry juice can be used as an oral contrast agent in upper abdominal MR for T1-weighted imaging. (orig.)

  20. A case report of a duodenal adenocarcinoma: a complication with Crohn's disease.

    Science.gov (United States)

    Sakakibara, Yuko; Yamada, Takuya; Kimura, Keiichi; Iwasaki, Ryuichirou; Iwasaki, Tetsuya; Ishihara, Akio; Nakazuru, Shoichi; Ishida, Hisashi; Kodama, Yoshinori; Mita, Eiji

    2016-03-01

    The prevalence of Crohn's disease (CD) in Japan is increasing, and so is the incidence of colorectal and small bowel cancers associated with CD. However, few reports have described the malignant transformation of duodenal lesions; moreover, such a diagnosis is rarely possible preoperatively. We present a case of malignant degeneration in the duodenal mucosa associated with CD. A 54-year-old man had been receiving treatment for CD for more than 20 years. Seven years ago, he was diagnosed with duodenal stenosis related to CD. He was asymptomatic, and biopsy results from the proximal stricture showed inflammatory changes without malignant transformation. The lesion was then monitored during follow-up. In 2013, he underwent an endoscopy, which revealed an ulcerated, nodular mucosa, immediately proximal to a high-grade obstruction of the descending duodenum. A biopsy of the ulcer lesion confirmed a diagnosis of adenocarcinoma. The patient then underwent duodenopancreatectomy. Histopathological results from the resected duodenum confirmed a poorly differentiated adenocarcinoma that had invaded the subserosa. The patient recovered, and no recurrence has been observed. Although the duodenum can be accessed without difficulty during endoscopy, it is challenging to preoperatively diagnose malignant transformation. There are only four reported cases of duodenal cancer stemming from CD-associated stricture, and only one of them received a preoperative diagnosis of malignancy based on endoscopic biopsy results. Progressive duodenal narrowing and ulceration in patients with CD should indicate a need for careful endoscopic evaluation and biopsy in order to exclude malignant degeneration of Crohn's duodenitis. Early diagnosis of cases of CD-associated cancers is necessary. We report the features of a rare and illustrative case of duodenal adenocarcinoma in a patient with CD.