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Sample records for dubius bledo pira

  1. 'PiraRoxa': cultivar de alface crespa de cor vermelha intensa 'PiraRoxa': triple red lettuce cultivar

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    Fernando César Sala; Cyro Paulino da Costa

    2005-01-01

    'PiraRoxa' é uma cultivar de alface (Lactuca sativa L.) crespa de cor vermelha intensa, resistente à Bremia lactucae, Thielaviopsis basicola e LMV, patótipo II. Foi selecionada por meio do método genealógico a partir do cruzamento entre o tipo Lolla Rossa 'RS 521998' e a cv. Gizela. Apresenta plantas grandes e vigorosas, folhas crespas, coloração vermelha intensa e brilhante. Em ensaios conduzidos no verão (2003/2004) o pendoamento da 'PiraRoxa' foi de 10 e 20 dias mais lento quando comparado...

  2. 'PiraRoxa': cultivar de alface crespa de cor vermelha intensa 'PiraRoxa': triple red lettuce cultivar

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    Fernando César Sala

    2005-03-01

    Full Text Available 'PiraRoxa' é uma cultivar de alface (Lactuca sativa L. crespa de cor vermelha intensa, resistente à Bremia lactucae, Thielaviopsis basicola e LMV, patótipo II. Foi selecionada por meio do método genealógico a partir do cruzamento entre o tipo Lolla Rossa 'RS 521998' e a cv. Gizela. Apresenta plantas grandes e vigorosas, folhas crespas, coloração vermelha intensa e brilhante. Em ensaios conduzidos no verão (2003/2004 o pendoamento da 'PiraRoxa' foi de 10 e 20 dias mais lento quando comparado às cvs. Banchu New Red Fire e Loretta, respectivamente. Trata-se da primeira cultivar tropicalizada para o segmento de alface vermelha com resistência a doenças no Brasil.'PiraRoxa' is a triple red leaf lettuce (Lactuca sativa L. cultivar resistant to Bremia lactucae, Thielaviopsis basicola and LMV, pathotype II. This cv. was obtained through pedigree selection from the cross between a Lolla Rossa 'RS 521998' and cv. Gizela. This shiny and intense red lettuce presents vigorous plants with loose leaves. In summer trials (2003/2004 'PiraRoxa' presented slow bolting performance (10 to 20 days later in comparison to cvs. Banchu New Red Fire and Loretta. This is the first tropicalized red leaf lettuce with multiple disease resistance developed in Brazil.

  3. Spermatogenesis, the mature sperm, and sperm egg association in Nematospiroides dubius

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    Clark, W. H., Jr.

    1974-01-01

    Nematode spermatogenesis was investigated using the strongyloid Nematospiroides dubius. The primary spermatocytes, development of spermatids, and changes in the sperm in the female tract are described.

  4. 'PiraRoxa': cultivar de alface crespa de cor vermelha intensa

    OpenAIRE

    Sala,Fernando César; Costa,Cyro Paulino da

    2005-01-01

    'PiraRoxa' é uma cultivar de alface (Lactuca sativa L.) crespa de cor vermelha intensa, resistente à Bremia lactucae, Thielaviopsis basicola e LMV, patótipo II. Foi selecionada por meio do método genealógico a partir do cruzamento entre o tipo Lolla Rossa 'RS 521998' e a cv. Gizela. Apresenta plantas grandes e vigorosas, folhas crespas, coloração vermelha intensa e brilhante. Em ensaios conduzidos no verão (2003/2004) o pendoamento da 'PiraRoxa' foi de 10 e 20 dias mais lento quando comparado...

  5. Eco-friendly synthesis of Graphene using the aqueous extract of Amaranthus dubius

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    M. Jannathul Firdhouse

    2013-06-01

    Full Text Available An eco-friendly process of reduction of graphene oxide using aqueous extract of Amaranthus dubius under refluxing method is herein reported. The colour change of the graphene oxide (GO solution from brown to black was noted during the reduction of graphene oxide. UV-Visible spectrophotometer was used to monitor the formation of reduced graphene oxide (AKRGO. The crystallite size of nanographene was confirmed by XRD analysis and Scherrer’s formula. FTIR spectral analysis revealed the reduction of graphene oxide using aqueous extract of Amaranthus dubius. The morphology of the synthesized graphene was examined by SEM analysis.

  6. Migration from food contact plastics. Part I. Establishment and aims of the PIRA project.

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    Tice, P A; McGuinness, J D

    1987-01-01

    In the European Community regulatory approaches adopted by individual Member States to the control of food contact plastics differ markedly and, as a result, the European Commission has identified a need for harmonized legislation in this sector. The Commission requested its Scientific Committee for Food to advise on the toxicity of monomers and other starting substances used in the production of such plastics and the Committee's report has recently been published. It is apparent that, although some monomers have been studied in depth, for many little or no information was available to the Committee on either the levels migrating into food or their likely toxic effects. The Committee has requested that such information be provided within 3 years. Following discussions between the UK Ministry of Agriculture, Fisheries and Food, PIRA and companies involved in either the manufacture or use of food contact plastics, a Project has been established at PIRA to develop sensitive analytical procedures for the relevant UK-required monomers and to provide the required migration data. As a second part to this project the methodology for determining overall migration is being examined with a view to resolving existing experimental difficulties. The first analytical methods to be developed in the project are those for the determination or terephthalic acid and isophthalic acid. Residual levels of these monomers in plastics and the quantities migrating into food simulants and food have been shown to be very low.

  7. Bromeliaceae from Pico Piraí, Guaratuba Municipality (Parana, Brazil Bromeliaceae Juss. do Pico Piraí, município de Guaratuba (Paraná, Brasil

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    Rosemeri Morokawa

    2011-12-01

    Full Text Available The results of a Bromeliaceae Juss. floristic survey in the Piraí Mountain, Araraquara complex(Guaratuba Municipality, Paraná State are presented. Twenty six species included in nine genera and three subfamilies were registered. Bromelioideae presented the highest genera diversity and Tillandsioideae showed the highest species diversity, including twelve species belonging to the genus Vriesea (46%. It was the first register of Nidularium procerum Lindm., Tillandsia stricta Sol. ex Ker Gawl., Vriesea altodaserrae L. B. Sm., Vriesea erythrodactylon (E. Morren E. Morren ex Mez, Vrieseaflava And.Costa, H. Luther & Wand., Vriesea friburgensis Mez, Vriesea hoehneana L. B. Sm., Vriesea inflata (Wawra Wawra e Wittrockia superba Lindm. at the Guaratuba Municipality. Dyckia leptostachya Baker was collected for the first time at the coastal zone of Paraná State, being this record done after 40 years since its last register in the State. For each species, information on phenology, geographic distribution and vulnerability level are included. Apresentam-se resultados de levantamento florístico das espécies de Bromeliaceae Juss. no Pico Piraí, Complexo do Araraquara (Guaratuba, Paraná. Verificou-se a ocorrência de 26 espécies pertencentes a nove gêneros e três subfamílias. Bromelioideae englobou a maior diversidade de gêneros e Tillandsioideae registrou-se a maior diversidade de espécies, sendo 12 delas incluídas no gênero Vriesea (46%. As seguintes espécies foram registradas pela primeira vez no município de Guaratuba: Nidularium procerum Lindm., Tillandsia stricta Sol. ex Ker Gawl., Vriesea altodaserrae L. B. Sm., Vriesea erythrodactylon (E. Morren E. Morren ex Mez, Vriesea flava And. Costa, H. Luther & Wand.,Vriesea friburgensis Mez, Vriesea hoehneana L. B. Sm., Vriesea inflata (Wawra Wawra e Wittrockia superba Lindm.. Dyckia leptostachya Baker foi registrada pela primeira vez na zona litorânea paranaense e após 40 anos do último

  8. Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats.

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    Rocha-E-Silva, Thomaz A A; Linardi, Alessandra; Antunes, Edson; Hyslop, Stephen

    2016-01-01

    Bites by tarantulas (Theraphosidae, Mygalomorphae) in humans can result in mild clinical manifestations such as local pain, erythema, and edema. Vitalius dubius is a medium-sized, nonaggressive theraphosid found in southeastern Brazil. In this work, we investigated the mediators involved in the plasma extravasation caused by V. dubius venom in rats. The venom caused dose-dependent (0.1-100 μg/site) edema in rat dorsal skin. This edema was significantly inhibited by ((S)1-{2-[3(3-4-dichlorophenyl)-1-(3-iso-propoxyphenylacetyl)piperidine-3-yl]ethyl}-4-phenyl-1-azoniabicyclo[2.2.2]octone, chloride) (SR140333, a neurokinin NK1 receptor antagonist), indomethacin [a nonselective cyclooxygenase (COX) inhibitor], cyproheptadine (a serotonin 5-hydroxytryptamine1/2 and histamine H1 receptor antagonist), and N(ω)-nitro-L-arginine methyl ester (a nitric oxide synthase inhibitor). In contrast, mepyramine (a histamine H1 receptor antagonist), D-Arg-[Hyp(3),Thi(5),D-Tic(7),Oic(8)-]-BK (JE 049, a bradykinin B2 receptor antagonist), and ((S)-N-methyl-N-[4-(4-acetylamino-4-phenylpiperidino)-2-(3,4-di-chlorophenyl)butyl]benzamide) (SR48968, a neurokinin NK2 receptor antagonist) had no effect on the venom-induced increase in vascular permeability. In rat hind paws, the venom-induced edema was attenuated by ketoprofen (a nonselective COX inhibitor) administered 15 minutes postvenom. Preincubation of venom with commercial antiarachnid antivenom attenuated the venom-induced edema. These results suggest that the enhanced vascular permeability evoked by V. dubius venom involves serotonin, COX products, neurokinin NK1 receptors, and nitric oxide formation. The attenuation of hind paw edema by ketoprofen suggests that COX inhibitors could be useful in treating the local inflammatory response to bites by these spiders.

  9. Effects of diets with Amaranthus dubius Mart. ex Thell. on performance and digestibility of growing rabbits

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    Edgar Molina

    2015-03-01

    Full Text Available The effects on performance and digestibility in growing rabbits were studied by comparing 3 diets containing increasing inclusion rates of amaranth (Amaranthus dubius Mart. ex Thell.: 0 (A0, 160 (A16 and 320 g/kg (A32 diet. Diets were formulated isoproteic and isocaloric to meet the nutrient requirements of growing rabbits. One hundred and thirteen weaned New Zealand White rabbits (mean±standard deviation weight: 760±102 g, individually caged, were randomly assigned to one of the 3  experimental diets. Rabbits were fed ad libitum from 35 to 87 d of age, and health status and performance traits were  onitored. The coefficients of total tract apparent digestibility of the diets were measured between 42 and 46 d of age in 12 rabbits per treatment. Amaranthus dubius contained 209 g/kg dry matter (DM of crude protein and 398 g/kg DM of neutral detergent fibre. There were no significant differences between treatments in weight gain (mean 21.6 g/d and live weight at the end of the fattening period (mean 1883 g. Daily feed intake was higher (P<0.05 in A0 than in A16 and A32 diets (85.4 vs. 73.7 and 69.9 g/d, respectively, and feed conversion rate improved with increased inclusion of A. dubius in the diet (from 3.84 to 3.28 for A0 and A32 diets, respectively; P<0.05. Health status was not affected by the amaranth inclusion rate. Total tract apparent digestibility showed high values, with no differences among diets except for ether extract. Thus, A. dubius could be considered as an alternative source of protein and fibre for rabbit feeding in tropical and subtropical regions.

  10. Purification and characterization of hyaluronidase from venom of the brazilian spider Vitalius dubius (Aranease, Theraphosidae)

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    Rafael Sutti

    2011-01-01

    Resumo: A hialuronidase (Hyase) de peçonha contribui para a difusão da peçonha do local da inoculação. Neste trabalho, foi purificada e caracterizada a Hyase da peçonha da Vitalius dubius (Araneae, Theraphosidae), uma aranha caranguejeira encontrada no sudeste do Brasil. A peçonha obtida por estimulação elétrica de machos e fêmeas adultas foi fracionada por cromatografia em gel filtração em uma coluna Superdex 75 (tampão acetato de sódio 0,1 M, pH 6, contendo 0,15 M de NaCl), a um fluxo de 1 ...

  11. Two New Tryptamine Derivatives, Leptoclinidamide and (--Leptoclinidamine B, from an Indonesian Ascidian Leptoclinides dubius

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    Michio Namikoshi

    2012-02-01

    Full Text Available Two new tryptamine-derived alkaloids, named as leptoclinidamide (1 and (--leptoclinidamine B (2, were isolated from an Indonesian ascidian Leptoclinides dubius together with C2-α-D-mannosylpyranosyl-L-tryptophan (3. The structure of 1 was assigned on the basis of spectroscopic data for 1 and its N-acetyl derivative (4. Compound 1 was an amide of tryptamine with two β-alanine units. Although the planar structure of 2 is identical to that of the known compound (+-leptoclinidamine B (5, compound 2 was determined to be the enantiomer of 5 based on amino acid analysis using HPLC methods. Compounds 1 to 4 were evaluated for cytotoxicity against two human cancer cell lines, HCT-15 (colon and Jurkat (T-cell lymphoma cells, but none of the compounds showed activity.

  12. Translocation and accumulation of Cr, Hg, As, Pb, Cu and Ni by Amaranthus dubius (Amaranthaceae) from contaminated sites.

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    Mellem, John J; Baijnath, Himansu; Odhav, Bharti

    2009-05-01

    Phytoremediation is an emerging technology where specially selected and engineered metal-accumulating plants are used for bioremediation. This study was undertaken to evaluate the potential of Amaranthus dubius for phytoremediation of chromium (Cr), mercury (Hg), arsenic (As), lead (Pb), copper (Cu) and nickel (Ni). Locally gathered soil and plants of A. dubius were investigated for the metals from a regularly cultivated area, a landfill site and a waste water treatment site. Metals were extracted from the samples using microwave-digestion and analyzed using Inductively Coupled Plasma-Mass Spectroscopy. The mode of phytoremediation, effect of the metals on the plants, ability of the plant to extract metals from soil (Bioconcentration Factor) and the ability of the plants to move the metals to the aerial parts of the plants (Translocation Factor) were evaluated. The survey of the three sites showed that soils were heavily contaminated with Cr, Hg, Cu and Ni. These levels were far above acceptable standards set for soils and above the standards set for the Recommended Dietary Allowance. Specimens of A. dubius from the three sites showed that they could tolerate Hg, sequester it from the soil, and translocate it to the shoots. Cr could only be removed from the soil and stored in the roots, with limited amounts translocated to the aerial parts. Pb, As, Ni, and Cu have some degree of transportability from the soil to the roots but not to aerial parts. The ability of A. dubius to be considered for phytoremediation has to be viewed with caution because translocation of the metals to the aerial parts of the plant is limited.

  13. Milking and partial characterization of venom from the Brazilian spider Vitalius dubius (Theraphosidae).

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    Rocha-E-Silva, Thomaz A A; Sutti, Rafael; Hyslop, Stephen

    2009-01-01

    The theraphosid spider genus Vitalius contains several species found in southeastern Brazil. In this work, we used electrostimulation to obtain venom from Vitalius dubius and examined its general composition. Male spiders yielded significantly less (p venom (12.5 +/- 0.7 mg of liquid/spider, n = 16; mean +/- S.E.M.) than female spiders (25.5 +/- 2.0 mg of liquid/spider, n = 11). However, when corrected for spider weight, males yielded slightly more venom (2.89 +/- 0.16 mg/g vs. 2.45 +/- 0.76 mg/g for males and females, respectively, p Venom yield correlated linearly with spider weight for spiders weighing up to approximately 12-13 g, but decreased in very heavy females. There was a marked decrease in venom yield after the first milking. The protein concentration of pooled venom was 18.3 +/- 2.4 mg/ml (n = 4) and accounted for 16.6 +/- 4.7% of the dry venom weight. The venom contained high hyaluronidase activity (275 +/- 24 TRU/mg of protein, n = 4), with a molecular mass of approximately 45 kDa estimated by zymography. SDS-PAGE revealed a few proteins with molecular masses >14 kDa but showed two staining bands of peptides venom reacted in ELISA with affinity-purified IgG from commercial arachnidic antivenom. Immunoblotting with this IgG detected proteins of 30-140 kDa only. Fractionation of the venom by reverse-phase chromatography resulted in five major and eight minor peaks.

  14. Net fluxes of electrolytes in the rat intestine infected with Moniliformis dubius (Acanthocephala).

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    Mettrick, D F; Budziakowski, M E; Podesta, R B

    1979-08-01

    The effect of Moniliformis dubius on fluxes of Na+, K+, Cl-, and HCO3-in the rat intestine was determined using a conventional in vivo single-pass perfusion technique. Results for ion and water movements in the uninfected gut were in agreement with previous studies. In the parasitized intestine the jejunal pH was significantly lower than that in control animals, matching the restriction of the parasites to this region of the small intestine. While parasitism did not affect Na+ transport in the distal ileum, Na+ absorption was reduced (pH 7.0), or secretion enhanced (pH 6.0), in the two proximal regions. Cl-absorption was reduced in the distal ileum, but secretion was enhanced in the other two segments. Parasitism also enhanced K+ secretion in all segments. Net H2O absorption was reduced at pH 7.0; at pH 7.0; at pH 6.0 net secretion was also reduced. These changes clearly indicate that a parasite restricted to the jejunum may significantly affect the absorptive and secretory activity of the intestine distal to the site of infection. The results are discussed in the light of current concepts of electrolyte transport. The effect of the parasites on mucosal function distal to their site of attachment is discussed in terms of the release by the parasite of toxin-like substances, changes in the physical-chemical characteristics of the intestinal lumen, and interference with neurohormonal control of gastrointestinal function.

  15. Halipegus dubius Klein, 1905 (Trematoda, Hemiuridae: a redescription, with notes on the working of the ovarian complex

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    W. Lobato Paraense

    1992-01-01

    Full Text Available A redescription of Halipegus dubius Klein, 1905, of Brazilian frogs Leptodactylus pentadactylus and L. ocellatus, is presented. The parasite was found exclusively in the buccal cavity, in number varying from 1 (in most instances to 3, and exceptionally 25 (one instance. Morphological data were based on 40 whole-mounted specimens and 4 serially sectioned ones. Larval development takes place in planorbid snails (Biomphalaria glabrata and B. tenagophila and in an undetermined species of cyclops. Working of the ovarian complex was followed by microscopical observation of life specimens. Constrating with the similarity of the four American species of Halipegus currently recognized as valid, they can be easily separated by the characters of their cercariae.

  16. VdTX-1, a reversible nicotinic receptor antagonist isolated from venom of the spider Vitalius dubius (Theraphosidae).

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    Rocha-E-Silva, Thomaz A A; Rostelato-Ferreira, Sandro; Leite, Gildo B; da Silva, Pedro Ismael; Hyslop, Stephen; Rodrigues-Simioni, Léa

    2013-08-01

    Theraphosid spider venoms can block neurotransmission in vertebrate nerve-muscle preparations in vitro, but few of the components involved have been characterized. In this work, we describe the neuromuscular activity of venom from the Brazilian theraphosid Vitalius dubius and report the purification and pharmacological characterization of VdTX-1, a 728 Da toxin that blocks nicotinic receptors. Neuromuscular activity was assayed in chick biventer cervicis preparations and muscle responses to exogenous ACh and KCl were determined before and after incubation with venom or toxin. Changes in membrane resting potential were studied in mouse diaphragm muscle. The toxin was purified by a combination of filtration through Amicon® filters, cation exchange HPLC and RP-HPLC; toxin purity and mass were confirmed by mass spectrometry. Venom caused progressive neuromuscular blockade and muscle contracture; the blockade but not the contracture was reversible by washing. Venom attenuated contractures to exogenous ACh and KCl. Filtration yielded low (LM, 5 kDa) fractions, with the latter reproducing the contracture seen in venom but with a slight and progressive twitch blockade. The LM fraction caused reversible blockade and attenuated contractures to ACh, but had no effect on contractures to KCl. VdTX-1 (728 Da) purified from the LM fraction was photosensitive and reduced the E(max) to ACh in biventer cervicis muscle without affecting the EC₅₀; VdTX-1 also abolished carbachol-induced depolarizations. V. dubius venom contains at least two components that affect vertebrate neurotransmission. One component, VdTX-1, blocks nicotinic receptors non-competitively to produce reversible blockade without muscle contracture.

  17. A tale of two very different comets: ISO and MSX measurements of dust emission from 126P/IRAS (1996) and 2P/Encke (1997)

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    Lisse, C. M.; Fernández, Y. R.; A'Hearn, M. F.; Grün, E.; Käufl, H. U.; Osip, D. J.; Lien, D. J.; Kostiuk, T.; Peschke, S. B.; Walker, R. G.

    2004-10-01

    We present the characteristics of the dust comae of two comets, 126P/IRAS, a member of the Halley family (a near-isotropic comet), and 2P/Encke, an ecliptic comet. We have primarily used mid- and far-infrared data obtained by the ISOPHOT instrument aboard the Infrared Space Observatory (ISO) in 1996 and 1997, and mid-infrared data obtained by the SPIRIT III instrument aboard the Midcourse Space Experiment (MSX) in 1996. We find that the dust grains emitted by the two comets have markedly different thermal and physical properties. P/IRAS's dust grain size distribution appears to be similar to that of fellow family member 1P/Halley, with grains smaller than 5 microns dominating by surface area, whereas P/Encke emits a much higher fraction of big (20 μm and higher) grains, with the grain mass distribution being similar to that which is inferred for the interplanetary dust population. P/Encke's dearth of micron-scale grains accounts for its visible-wavelength classification as a "gassy" comet. These conclusions are based on analyses of both imaging and spectrophotometry of the two comets; this combination provides a powerful way to constrain cometary dust properties. Specifically, P/IRAS was observed preperihelion while 1.71 AU from the Sun, and seen to have a 15-arcmin long mid-infrared dust tail pointing in the antisolar direction. No sunward spike was seen despite the vantage point being nearly in the comet's orbital plane. The tail's total mass at the time was about 8×10 9 kg. The spectral energy distribution (SED) is best fit by a modified greybody with temperature T=265±15 K and emissivity ɛ proportional to a steep power law in wavelength λ: ɛ∝ λ- α, where α=0.50±0.20 (2σ) . This temperature is elevated with respect to the expected equilibrium temperature for this heliocentric distance. The dust mass loss rate was between 150-600 kg/s (95% confidence), the dust-to-gas mass loss ratio was about 3.3, and the albedo of the dust was 0.15±0

  18. Comparative Study of Betacyanin Profile and Antimicrobial Activity of Red Pitahaya (Hylocereus polyrhizus) and Red Spinach (Amaranthus dubius).

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    Yong, Yi Yi; Dykes, Gary; Lee, Sui Mae; Choo, Wee Sim

    2017-03-01

    Betacyanins are reddish to violet pigments that can be found in red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius). This study investigated the impact of sub-fractionation (solvent partitioning) on betacyanin content in both plants. Characterization of betacyanins and evaluation of their antimicrobial activities were also carried out. Betanin was found in both plants. In addition, isobetanin, phyllocactin and hylocerenin were found in red pitahaya whereas amaranthine and decarboxy-amaranthine were found in red spinach. Sub-fractionated red pitahaya and red spinach had 23.5 and 121.5 % more betacyanin content, respectively, than those without sub-fractionation. Sub-fractionation increased the betanin and decarboxy-amaranthine content in red pitahaya and red spinach, respectively. The betacyanin fraction from red spinach (minimum inhibitory concentration [MIC] values: 0.78-3.13 mg/mL) demonstrated a better antimicrobial activity profile than that of red pitahaya (MIC values: 3.13-6.25 mg/mL) against nine Gram-positive bacterial strains. Similarly, the red spinach fraction (MIC values: 1.56-3.13 mg/mL) was more active than the red pitahaya fraction (MIC values: 3.13-6.25 mg/mL) against five Gram-negative bacterial strains. This could be because of a higher amount of betacyanin, particularly amaranthine in the red spinach.

  19. Efecto de la época de recolecta y órgano de la planta sobre el contenido de metales de Amaranthus Dubius Mart. ex Thell

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    González Redondo, Pedro; Molina, Edgar; Montero, Keyla; Moreno Rojas, Rafael; Sánchez-Urdaneta, Adriana Beatriz

    2011-01-01

    El amaranto (Amaranthus dubius Mart. ex Thell.) es utilizado como planta forrajera en la alimentación de ovinos, caprinos, porcinos y bovinos, además, de ser reportada como arvense en diversos cultivos comerciales. Se evaluó el contenido de metales en hojas, tallos y panículas de amaranto recolectado en época lluviosa y seca. Las plantas fueron cultivadas en la población de Merecure, municipio Acevedo, estado Miranda, Venezuela. Se determinó la concentración de Ca, Mg, Zn, Fe, Cu, Al, Cd, Pb ...

  20. Estimativas de parâmetros populacionais e demográficos de Ozotoceros bezoarticus (Artiodactyla, Cervidae em Piraí do Sul, Paraná, sul do Brasil Estimates of population and demographic parameters of Ozotoceros bezoarticus (Artiodactyla, Cervidae in Piraí do Sul, Paraná, southern Brazil

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    Fernanda G. Braga

    2010-06-01

    Full Text Available Este estudo foi realizado em duas propriedades particulares, situadas no município de Piraí do Sul, sul do Brasil. Foram realizadas 16 fases de campo mensais, com três dias de duração, no período compreendido entre fevereiro de 2001 e maio de 2002, com o objetivo de obter informações sobre a biologia do veado-campeiro (Ozotoceros bezoarticus Linnaeus, 1758 e identificar os principais fatores de impacto sobre o mesmo. Foram computadas 1.065 observações, estimando-se 71,45 indivíduos na população, sendo o tamanho médio dos grupos de 2,29 (DV ± 0,55, e a razão sexual de 0,83. Indivíduos isolados corresponderam a 40% das observações, sendo o maior grupo composto por 10 indivíduos. Registrou-se um pico de nascimentos entre setembro e novembro, embora esses tenham acontecido ao longo de todo o estudo. Foram registrados 34 óbitos, correspondendo a uma mortalidade de 47,6%, sendo as principais causas a predação por Puma concolor (Linnaeus, 1771, a caça e os atropelamentos. A população estudada encontra-se severamente ameaçada, e não sobreviverá por muito tempo a menos que políticas que garantam a sua conservação sejam adotadas. Estas políticas devem incluir o manejo adequado das áreas e o controle dos vetores de pressão.This study was conducted in two private properties in the municipality of Piraí do Sul (Paraná state, southern Brazil. Sixteen monthly visits were made each one lasting three days between February 2001 and May 2002. The study aimed at the observation of pampas deer biology (Ozotoceros bezoarticus Linnaeus, 1758, and the evaluation of main impacts to this population. We computed 1,065 observations of estimated 71.45 individuals. The average size of the groups was 2.29 (SD ± 0.55 animals, and the sexual ratio was 0.83. Isolated deer accounted for 40% of the observations, whereas the largest group comprised 10 individuals. It was recorded a peack period of births between September and November, although

  1. Efectos del consumo de panes integrales elaborados con harina de Amaranthus dubius Mart. ex Thell. y harina de trigo en ratas con sídrome metabólico

    OpenAIRE

    Montero Quiroga, Keyla Carolina

    2014-01-01

    El género Amaranthus se caracteriza por su alto contenido de nutrientes comparables a los alimentos de origen animal. Sin embargo, la especie Amaranthus dubius, la cual está diseminada en Venezuela, se considera un arvense de cultivos de subsistencia, como el maíz, sorgo y leguminosas; esto impulso la investigación sobre la posible aplicación del amaranto venezolano para la alimentación humana. El estudio se inició con la evaluación de la composición proximal, el contenid...

  2. Efectos del consumo de panes integrales elaborados con harina de Amaranthus dubius Mart. ex Thell. y harina de trigo en ratas con sídrome metabólico

    OpenAIRE

    Montero Quiroga, Keyla Carolina

    2014-01-01

    El género Amaranthus se caracteriza por su alto contenido de nutrientes comparables a los alimentos de origen animal. Sin embargo, la especie Amaranthus dubius, la cual está diseminada en Venezuela, se considera un arvense de cultivos de subsistencia, como el maíz, sorgo y leguminosas; esto impulso la investigación sobre la posible aplicación del amaranto venezolano para la alimentación humana. El estudio se inició con la evaluación de la composición proximal, el contenid...

  3. [Effect of consumption of bread with amaranth (Amaranthus dubius Mart. ex Thell.) on glycemic response and biochemical parameters in Sprague dawley rats].

    Science.gov (United States)

    Montero-Quintero, Keyla Carolina; Moreno-Rojas, Rafael; Molina, Edgar Alí; Colina-Barriga, Máximo Segundo; Sánchez-Urdaneta, Adriana Beatriz

    2014-11-01

    Introducción: La incorporación de ingredientes funcionales como el amaranto (Amaranthus dubius Mart. ex Thell.) en la elaboración de pan es una estrategia para aumentar el consumo de fibra, el cual está relacionado con efectos beneficiosos para la salud, mejorando la respuesta glicémica y el perfil lipídico. Materiales y métodos: Treinta ratas machos Sprague dawley se distribuyeron al azar en tres grupos: dieta de pan con 0% de amaranto (PA0, control), dieta de pan con 10% de amaranto (PA10) y dieta de pan con 20% de amaranto (PA20) para determinar el consumo de alimento, ganancia en peso, triglicéridos, colesterol total, VLDL-C, LDL-C, HDL-C, proteínas y la respuesta glicémica postpandrial. Los datos fueron analizados a través de un análisis completamente aleatorizado con 10 repeticiones, utilizando la prueba de comparación de medias de Tukey para los parámetros bioquímicos. La respuesta glicémica postprandial fue analizada por el método de medidas repetidas en el tiempo. Resultados y discusión: La ingesta diaria y la ganancia de peso no se afecto (p>0,05) en los grupos con PA10 y PA20. La concentración de glucosa, triglicéridos y proteína presentó diferencias estadísticamente significativas (p>0,05) por la diferencia de contenido de amaranto de las dietas. Los valores de colesterol total, LDL-C, factor de riesgo e índice aterogénico presentaron diferencias estadísticamente significativas (p>0,05) resultando el grupo de menor valor el formado por PA10 y PA20. En los grupos PA10 y PA20 el pico de hiperglucemia y la fase de hiperglucemia total fue más bajo, mostrando una mejor respuesta glicémica. Conclusión: el amaranto podría ser utilizado como ingrediente funcional en la elaboraciones de panes ya que permitió mejorar el perfil lipídico así como la respuesta glicémica postpandrial.

  4. Evaluación de extractos de fique, coquito, sorgo y ruda como posibles bio-herbicidas Evaluation of cuban hemp, nut sedge, johnson grass and herb of grace extracts in weed control

    OpenAIRE

    Liliana Osorio Salazar; Francisco Antonio Valverde; Carmen Rosa Bonilla Correa; Manuel Salvador Sánchez Orozco; Carmen Elena Mier Barona

    2009-01-01

    En experimentos completamente al azar en condiciones de laboratorio y campo, en Palmira, Valle del Cauca, se evaluó el efecto de extractos de Macrophylla furcraea Baker (fique), Cyperus rotundus L (coquito), Sorghum bicolor L (sorgo), y Ruta graveolens L (ruda) sobre la germinación de semillas de las arvenses Bidens pilosa L (papunga) y Amaranthus dubius Mart (bledo) y del cilantro Coriandrum sativum L. Se obtuvieron los extractos por el método Soxhlet, utilizando agua, etanol y cloroformo co...

  5. Evaluación de extractos de fique, coquito, sorgo y ruda como posibles bio-herbicidas Evaluation of cuban hemp, nut sedge, johnson grass and herb of grace extracts in weed control

    OpenAIRE

    Liliana Osorio Salazar; Francisco Antonio Valverde; Carmen Rosa Bonilla Correa; Manuel Salvador Sánchez Orozco; Carmen Elena Mier Barona

    2009-01-01

    En experimentos completamente al azar en condiciones de laboratorio y campo, en Palmira, Valle del Cauca, se evaluó el efecto de extractos de Macrophylla furcraea Baker (fique), Cyperus rotundus L (coquito), Sorghum bicolor L (sorgo), y Ruta graveolens L (ruda) sobre la germinación de semillas de las arvenses Bidens pilosa L (papunga) y Amaranthus dubius Mart (bledo) y del cilantro Coriandrum sativum L. Se obtuvieron los extractos por el método Soxhlet, utilizando agua, etanol y cloroformo co...

  6. Papá Noel en la pira (Father Christmas on the Pyre

    Directory of Open Access Journals (Sweden)

    Claude Lévi-Strauss

    2010-05-01

    Full Text Available En “Papá Noel en la pira”, Lévi-Strauss desdobla la aproximación al noble anciano en una pesquisa histórica y en otra etnológica. La primera lo lleva a leyendas medievales en las que sobreviven festividades del Imperio Romano y oscuras celebraciones en las que niños y muertos se confunden. La segunda sumerge al lector en una especulación acerca de la relación entre los vivos y los muertos que toma por excusa las katchina de los indios de Norteamérica. Al cabo, constata que esta figura mediadora enmascara la presencia de la muerte y tiende a negarla.

  7. Arte funerario y astrología: La Pira de Luis I

    OpenAIRE

    Sebastián López, Santiago

    1991-01-01

    LOS FUNERALES DE EPOCAS ANTIGUAS TENDIERON A USAR ELOCUENTES IMAGENES ASTROLOGICAS QUE HACEN REFERENCIA AL MAS ALLA. EL ARTICULO ANALIZA EL CATAFALCO DE LUIS I (1724), PRIMERA EXHIBICION COMPLETA DE IMAGENES ASTROLOGICAS EN EL ARTE EFIMERO DEL MEXICO COLONIAL. (A.B.C./ L.A.G.)

  8. Evaluación de extractos de fique, coquito, sorgo y ruda como posibles bio-herbicidas

    Directory of Open Access Journals (Sweden)

    Liliana Osorio Salazar

    2009-04-01

    Full Text Available En experimentos completamente al azar en condiciones de laboratorio y campo, en Palmira, Valle del Cauca, se evaluó el efecto de extractos de Macrophylla furcraea Baker (fique, Cyperus rotundus L (coquito, Sorghum bicolor L (sorgo, y Ruta graveolens L (ruda sobre la germinación de semillas de las arvenses Bidens pilosa L (papunga y Amaranthus dubius Mart (bledo y del cilantro Coriandrum sativum L. Se obtuvieron los extractos por el método Soxhlet, utilizando agua, etanol y cloroformo como solventes. Los extractos obtenidos se evaluaron en tres diluciones (0, 5 y 10 % en pruebas de germinación de semillas de las arvenses y del cultivo. Se emplearon tres repeticiones de 50 semillas cada una y el testigo se regó con agua destilada. Los extractos etanólicos y clorofórmicos de coquito y fique en las diluciones al 5 y 10 % presentaron el mayor efecto inhibitorio en la germinación a los 21 días. El ensayo de campo demostró el efecto inhibitorio de los extractos etanólicos de fique y coquito en la emergencia de las semillas del cilantro. El análisis de metabolitos secundarios comprobó la presencia de compuestos reportados como altamente tóxicos en fique y en menor cantidad en coquito, aunque los extractos de éste fueron los que más inhibieron la germinación.

  9. Biology and conservation status of Piraja’s Lancehead Snake Bothrops piraña Amaral, 1923 (Serpentes: Viperidae, Brazil

    Directory of Open Access Journals (Sweden)

    M.A.D. Freitas

    2014-09-01

    Full Text Available The venomous snake Bothrops pirajai (Amaral, 1923 is endemic to Brazil. Despite being described almost a century ago, very little is known about this species, which has never been studied in situ. Here, we present new data on the biology and natural history of B. pirajai based on a review of existing museum specimens and a field study (1504 man hours carried out throughout the species range. The distribution of B. pirajai was found to be restricted to the eastern Atlantic forest of the state of Bahia, Brazil, between Todosos Santos Bay, Itabuna and Ilhéus (12050’S-14050’S, 88-835 m. We recommend the species be uplisted to Endangered in the International Union for the Conservation of Nature’s global Red List of Threatened Species as its estimated extent of occurrence is under 5000km2. The effective conservation of B. pirajai will rely on addressing two key issues: improving our knowledge of the species and successfully mitigating habitat loss and fragmentation.

  10. Biology and conservation status of Piraja’s Lancehead Snake Bothrops piraña Amaral, 1923 (Serpentes: Viperidae, Brazil

    Directory of Open Access Journals (Sweden)

    M.A.D. Freitas

    2014-09-01

    Full Text Available The venomous snake Bothrops pirajai (Amaral, 1923 is endemic to Brazil. Despite being described almost a century ago, very little is known about this species, which has never been studied in situ. Here, we present new data on the biology and natural history of B. pirajai based on a review of existing museum specimens and a field study (1504 man hours carried out throughout the species range. The distribution of B. pirajai was found to be restricted to the eastern Atlantic forest of the state of Bahia, Brazil, between Todosos Santos Bay, Itabuna and Ilhéus (12050’S-14050’S, 88-835 m. We recommend the species be uplisted to Endangered in the International Union for the Conservation of Nature’s global Red List of Threatened Species as its estimated extent of occurrence is under 5000km2. The effective conservation of B. pirajai will rely on addressing two key issues: improving our knowledge of the species and successfully mitigating habitat loss and fragmentation.

  11. The northward shifting neophyte Tragopogon dubius is just as effective in forming mycorrhizal associations as the native T. pratensis.

    NARCIS (Netherlands)

    van Grunsven, R.; Yuwati, T.; Kowalchuk, George; van der Putten, W.H.; van Veenendaal, E.

    2014-01-01

    Background: As a consequence of climate warming, many organisms are shifting their range towards higher latitudes and altitudes. As not all do so at the same speed, this may disrupt biotic interaction. Release from natural enemies through range expansion can result in invasiveness, whereas loss of m

  12. The northward shifting neophyte Tragopogon dubius is just as effective in forming mycorrhizal associations as the native T. pratensis

    NARCIS (Netherlands)

    Grunsven, van R.H.A.; Yuwati, T.; Kowalchuk, G.A.; Putten, van der W.H.; Veenendaal, E.

    2014-01-01

    Background: As a consequence of climate warming, many organisms are shifting their range towards higher latitudes and altitudes. As not all do so at the same speed, this may disrupt biotic interaction. Release from natural enemies through range expansion can result in invasiveness, whereas loss of m

  13. Evaluación de extractos de fique, coquito, sorgo y ruda como posibles bio-herbicidas

    Directory of Open Access Journals (Sweden)

    Mier Barona Carmen Elena

    2009-06-01

    Full Text Available En experimentos completamente al azar en condiciones de laboratorio y campo, en Palmira, Valle del Cauca, se evaluó el efecto de extractos de Macrophylla furcraea Baker (fique, Cyperus rotundus L (coquito, Sorghum bicolor L (sorgo, y Ruta graveolens L (ruda sobre la germinación de semillas de las arvenses Bidens pilosa L (papunga y Amaranthus dubius Mart (bledo y del cilantro Coriandrum sativum L. Se obtuvieron los extractos por el método Soxhlet, utilizando agua, etanol y cloroformo como solventes. Los extractos obtenidos se evaluaron en tres diluciones (0, 5 y 10 % en pruebas de germinación de semillas de las arvenses y del cultivo. Se emplearon tres repeticiones de 50 semillas cada una y el testigo se regó con agua destilada. Los extractos etanólicos y clorofórmicos de coquito y fique en las diluciones al 5 y 10 % presentaron el mayor efecto inhibitorio en la germinación a los 21 días. El ensayo de campo demostró el efecto inhibitorio de los extractos etanólicos de fique y coquito en la emergencia de las semillas del cilantro. El análisis de metabolitos secundarios comprobó la presencia de compuestos reportados como altamente tóxicos en fique y en menor cantidad en coquito, aunque los extractos de éste fueron los que más inhibieron la germinación.

  14. Risk factors for diarrhea in a cohort of dairy heifer calves reared in individual outdoor hutches in Piraí, Rio de Janeiro, Brazil

    National Research Council Canada - National Science Library

    Thais Ferreira Fagundes; Letícia Gabriela Poblete Vidal; Pedro Afonso Moreira Alves; Wagner de Souza Tassinari; Rita de Cássia Alves Alcântara de Menezes; Adevair Henrique da Fonseca; Maria Julia Salim Pereira

    2016-01-01

    This study aimed to evaluate the association between diarrhea and risk factors potentially related to diarrhea incidence, such as Cryptosporidium, Giardia, Eimeria and nematode infection, animal age...

  15. Chlorofluorocarbons (CFC's): Regulation and replacement. (Latest citations from the PIRA Ppackaging, Paper, Printing, and Publishing, and Nonwovens abstracts database). Published Search

    Energy Technology Data Exchange (ETDEWEB)

    1993-11-01

    The bibliography contains citations concerning chlorofluorocarbons (CFC's), and efforts to find substitute materials in response to the need to lessen the environmental impact of CFC on the ozone layer, as agreed to in the international Montreal Protocol. CFC alternatives are expected to be used as aerosol propellants, blowing agents, refrigerants, fire extinguishants, and solvents. References to the effects of CFC on ozone are found in separate bibliographies. (Contains 250 citations and includes a subject term index and title list.)

  16. “Sou caipira pira pora...”: representations of the provincial, from the parasite of the land to the paradigm of national reality (1889-1945“Sou caipira pira pora...”: representações sobre o caboclo, do parasita da terra a paradigma da realidade nacional (1889-1945

    Directory of Open Access Journals (Sweden)

    Jonathan de Oliveira Molar

    2009-04-01

    Full Text Available This paper is aimed at discussing the representations about the hillbilly, the national peasant based on visions of two intellectuals: writer Monteiro Lobato and painter José Ferraz de Almeida Jr. Therefore, both literature and iconographic sources will support an analysis of the living standards of the rustic, as found in the historical period covered – 1889 until 1945. Contributions from authors who work with image analysis will be used as method, such as Henry Silva’s and Ana M. Mauad’s, and authors who work with the said period and the provincial issue: Lilia Schwarcz, Glauco Barsalini and Antonio Candido. O presente trabalho se propõe a discutir as representações elaboradas acerca do caboclo, do caipira nacional a partir das visões de dois intelectuais: o escritor Monteiro Lobato e o pintor José Ferraz de Almeida Júnior. Desse modo, analisar tanto na literatura quanto no conjunto iconográfico o ir e vir que perpassa as considerações sobre o modo de vida do caboclo, de acordo com o período histórico abordado – de 1889 até 1945. Para tanto, utiliza-se de autores que trabalham com análise de imagens, tais como: Henrique Silva e Ana M. Mauad; e, autores que trabalham com o citado período e a questão cabocla: Lilia Schwarcz, Glauco Barsaline e Antônio Cândido.

  17. Evaluación de extractos de fique, coquito, sorgo y ruda como posibles bio-herbicidas Evaluation of cuban hemp, nut sedge, johnson grass and herb of grace extracts in weed control

    Directory of Open Access Journals (Sweden)

    Liliana Osorio Salazar

    2009-04-01

    Full Text Available En experimentos completamente al azar en condiciones de laboratorio y campo, en Palmira, Valle del Cauca, se evaluó el efecto de extractos de Macrophylla furcraea Baker (fique, Cyperus rotundus L (coquito, Sorghum bicolor L (sorgo, y Ruta graveolens L (ruda sobre la germinación de semillas de las arvenses Bidens pilosa L (papunga y Amaranthus dubius Mart (bledo y del cilantro Coriandrum sativum L. Se obtuvieron los extractos por el método Soxhlet, utilizando agua, etanol y cloroformo como solventes. Los extractos obtenidos se evaluaron en tres diluciones (0, 5 y 10 % en pruebas de germinación de semillas de las arvenses y del cultivo. Se emplearon tres repeticiones de 50 semillas cada una y el testigo se regó con agua destilada. Los extractos etanólicos y clorofórmicos de coquito y fique en las diluciones al 5 y 10 % presentaron el mayor efecto inhibitorio en la germinación a los 21 días. El ensayo de campo demostró el efecto inhibitorio de los extractos etanólicos de fique y coquito en la emergencia de las semillas del cilantro. El análisis de metabolitos secundarios comprobó la presencia de compuestos reportados como altamente tóxicos en fique y en menor cantidad en coquito, aunque los extractos de éste fueron los que más inhibieron la germinación.The objective of this work was to evaluate the effect of the extracts of the species Macrophylla furcraea Baker (cuban hemp, Cyperus rotundus L (nut sedge, Sorghum bicolor L (johnson grass and Ruta graveolens L (herb of grace on seed germination of two weeds and one crop. The extracts were obtained by the Soxhlet method, using water, ethanol and chloroform as solvents. Each extract was evaluated in three dilutions (0, 5 and 10 % v/v and was applied to Bidens pilosa L (spanish needle, Amaranthus dubius Mart (spleen amaranth and Coriandrum sativum L (coriander seeds. Three replications of 50 seeds each one were used and the control was watered only with destilled water. The results

  18. PirAB toxin from Photorhabdus asymbiotica as a larvicide against dengue vectors.

    Science.gov (United States)

    Ahantarig, Arunee; Chantawat, Nantarat; Waterfield, Nicholas R; ffrench-Constant, Richard; Kittayapong, Pattamaporn

    2009-07-01

    We have evaluated Photorhabdus insect-related protein (Pir) from Photorhabdus asymbiotica against dengue vectors. PirAB shows larvicidal activity against both Aedes aegypti and Aedes albopictus larvae but did not affect the Mesocyclops thermocyclopoides predator. PirAB expressed the strongest toxicity compared to PirA, PirB, or the mixture of PirA plus PirB. Whether the presence of an enterobacterial repetitive intergenic consensus sequence in PirAB, but not in PirA, PirB, or the mixture of PirA plus PirB, has any impact on biological control efficacy needs further investigation.

  19. Protein (Cyanobacteria): 249773 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ZP_09782242.1 1117:4644 1150:2740 35823:86 376219:14 transposase (fragment) Arthros...pira sp. PCC 8005 MAQLWDDDISPRTISRALKKIGFTRKKTYGYQERWKQQREEFMAQIEQMEPEGLVYLDEAGMNSQDSDYPYGYCQEGQRFGALKSGKRQG

  20. Protein (Cyanobacteria): 249820 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ZP_09780023.1 1117:4644 1150:2740 35823:86 376219:14 transposase (fragment) Arthros...pira sp. PCC 8005 MAELWDDDISPRTISRALKKIGFTRKKTYGYQERWKQQREELIAQIEQMEPEGLVYLDEAGMNSSDSDYPYGYCEEGTTLPMPSNQGRGKGRGELYGPRGLNNNFLTPL ...

  1. Protein (Cyanobacteria): 249770 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ZP_09782144.1 1117:4644 1150:2740 35823:86 376219:14 transposase (fragment) Arthros...pira sp. PCC 8005 MAELWDDDISPRTISRALKKIGFTRKKTYGYQERWKQQREEFMAQIEQMEPQEVVYLDEAGMNSQDSDYPYGYCEEGKRFHALKSGKRQG

  2. Protein (Cyanobacteria): 249781 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ZP_09781797.1 1117:4644 1150:2740 35823:86 376219:14 transposase (fragment) Arthros...pira sp. PCC 8005 MKKIGFTRKKTYGYQERDEQQREEFMAQIEQMEPQEVVYLDEAGMNSQDSDYPYGYCEEGKRFHALKSGKRQGRVSMIAAWCHQQLLAPF

  3. Protein (Cyanobacteria): 144862 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ded protein LtrA-like (Includes: Reverse-transcriptase ; RNA maturase ; HNH endonuclease) (fragment) Arthros...pira sp. PCC 8005 MNLSRILRETSKQNHYDGCGFQNPAGMKKAGMGIPTIQDRAKQALVKSALEPEWESRFEGTSYGFRPGRSAQDAIARIYLCINHSDYYVLDADIACETFRSEIGLQCLK ...

  4. Protein (Cyanobacteria): 249776 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ZP_09783561.1 1117:4644 1150:2740 35823:86 376219:14 transposase (fragment) Arthros...pira sp. PCC 8005 MKKIGFTRKKTYGYQERWKQQREEFIAQIEQMEPEGLVYLDEAGMNSQDSDYPYGYCEEGKRFHALKSGKRQGRVSYMAPWCDQQLLAPF

  5. Protein (Cyanobacteria): 144863 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ded protein LtrA-like (Includes: Reverse-transcriptase ; RNA maturase ; HNH endonuclease) (fragment) Arthros...pira sp. PCC 8005 MNWLRALREISKPNHCDGCGFQNLEGMKKAGMGIPTIQDRARQALVKSALEPEWESRFEGTSYGFRPGRSAQDAIARIYSSINKGEYFVLDAGARRSGMK ...

  6. Protein (Cyanobacteria): 249772 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ZP_09781578.1 1117:4644 1150:2740 35823:86 376219:14 transposase (fragment) Arthros...pira sp. PCC 8005 MAELWDDDISPRTISRALKKIGFTRKKTYGYQERWKQQREEFMAQIEQMEPQEVVYLDEAGMNSQDSDYPYGYCEEGKRFHALKSGKRQG

  7. PirAB Toxin from Photorhabdus asymbiotica as a Larvicide against Dengue Vectors▿

    OpenAIRE

    Ahantarig, Arunee; Chantawat, Nantarat; Waterfield, Nicholas R.; ffrench-Constant, Richard; Kittayapong, Pattamaporn

    2009-01-01

    We have evaluated Photorhabdus insect-related protein (Pir) from Photorhabdus asymbiotica against dengue vectors. PirAB shows larvicidal activity against both Aedes aegypti and Aedes albopictus larvae but did not affect the Mesocyclops thermocyclopoides predator. PirAB expressed the strongest toxicity compared to PirA, PirB, or the mixture of PirA plus PirB. Whether the presence of an enterobacterial repetitive intergenic consensus sequence in PirAB, but not in PirA, PirB, or the mixture of P...

  8. Talcum Powder and Cancer

    Science.gov (United States)

    ... would not have the same type of potential bias, have not found an increased risk. For any ... well as journalists, editors, and translators with extensive experience in medical writing. Coggiola M, Bosio D, Pira ...

  9. Development and Validation of RP-LC Method for the Determination of Cinnarizine/Piracetam and Cinnarizine/Heptaminol Acefyllinate in Presence of Cinnarizine Reported Degradation Products.

    Science.gov (United States)

    El-Houssini, Ola M; Zawilla, Nagwan H; Mohammad, Mohammad A

    2013-01-01

    Specific stability indicating reverse-phase liquid chromatography (RP-LC) assay method (SIAM) was developed for the determination of cinnarizine (Cinn)/piracetam (Pira) and cinnarizine (Cinn)/heptaminol acefyllinate (Hept) in the presence of the reported degradation products of Cinn. A C18 column and gradient mobile phase was applied for good resolution of all peaks. The detection was achieved at 210 nm and 254 nm for Cinn/Pira and Cinn/Hept, respectively. The responses were linear over concentration ranges of 20-200, 20-1000 and 25-1000 μgmL(-1) for Cinn, Pira, and Hept respectively. The proposed method was validated for linearity, accuracy, repeatability, intermediate precision, and robustness via statistical analysis of the data. The method was shown to be precise, accurate, reproducible, sensitive, and selective for the analysis of Cinn/Pira and Cinn/Hept in laboratory prepared mixtures and in pharmaceutical formulations.

  10. Portanus Ball: descrição de uma espécie nova (Hemiptera, Cicadellidae, Xestocephalinae

    Directory of Open Access Journals (Sweden)

    Adenomar Neves de Carvalho

    2004-01-01

    Full Text Available Portanus dubius sp. nov. é descrita e ilustrada a partir de espécimens que foram coletados com armadilha Malaise durante um levantamento entomológico no Estado do Paraná, Brasil (PROFAUPAR.Portanus Ball: description of a new species (Hemiptera, Cicadellidae, Xestocephalinae. Portanus dubius sp. nov. is described and illustrated. The specimens were collected with Malaise trap during an entomological inventory in Paraná State, Brazil (PROFAUPAR.

  11. Analysis of the genome of a Korean isolate of the Pieris rapae granulovirus enabled by its separation from total host genomic DNA by pulse-field electrophoresis.

    Directory of Open Access Journals (Sweden)

    Yong Hun Jo

    Full Text Available BACKGROUND: Most traditional genome sequencing projects involving viruses include the culture and purification of the virus particles. However, purification of virions may yield insufficient material for traditional sequencing. The electrophoretic method described here provides a strategy whereby the genomic DNA of the Korean isolate of Pieris rapae granulovirus (PiraGV-K could be recovered in sufficient amounts for sequencing by purifying it directly from total host DNA by pulse-field gel electrophoresis (PFGE. METHODOLOGY/PRINCIPAL FINDINGS: The total genomic DNA of infected P. rapae was embedded in agarose plugs, treated with restriction nuclease and methylase, and then PFGE was used to separate PiraGV-K DNA from the DNA of P. rapae, followed by mapping of fosmid clones of the purified viral DNA. The double-stranded circular genome of PiraGV-K was found to encode 120 open reading frames (ORFs, which covered 92% of the sequence. BLAST and ORF arrangement showed the presence of 78 homologs to other genes in the database. The mean overall amino acid identity of PiraGV-K ORFs was highest with the Chinese isolate of PiraGV (~99%, followed up with Choristoneura occidentalis ORFs at 58%. PiraGV-K ORFs were grouped, according to function, into 10 genes involved in transcription, 11 involved in replication, 25 structural protein genes, and 15 auxiliary genes. Genes for Chitinase (ORF 10 and cathepsin (ORF 11, involved in the liquefaction of the host, were found in the genome. CONCLUSIONS/SIGNIFICANCE: The recovery of PiraGV-K DNA genome by pulse-field electrophoretic separation from host genomic DNA had several advantages, compared with its isolation from particles harvested as virions or inclusions from the P. rapae host. We have sequenced and analyzed the 108,658 bp PiraGV-K genome purified by the electrophoretic method. The method appears to be generally applicable to the analysis of genomes of large viruses.

  12. Tectono-volcanic control of fissure type vents for the 28 Ma Panalillo ignimbrite in the Villa de Reyes Graben, San Luis PotosI, Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Tristan-Gonzalez, Margarito; Labarthe-Hernandez, Guillermo; Aguillon-Robles, Alfredo [Instituto de Geologia/DES IngenierIa, UASLP, Av. Dr. Manuel Nava 5, Zona Universitaria, C.P. 78240, San Luis PotosI, S.L.P. (Mexico); Aguirre-DIaz, Gerardo J [Centro de Geociencias, Universidad Nacional Autonoma de Mexico, Campus Juriquilla, Queretaro, 76230 (Mexico)], E-mail: mtiistan@uasln.mx, E-mail: ger@geociencias.unam.mx

    2008-10-01

    The volcano-tectonic events at the Villa de Reyes Graben (VRG), in the southern Sierra Madre Occidental, Mexico, include 1) a regional NNE fault system developed before 32 Ma, 2) this pre-32 Ma faulting controlled the emplacement of 31.5 Ma dacitic domes, 3) NE faulting at 28 Ma that displaced the 31.5 Ma dacitic domes and formed the VRG, as well as the oblique grabens of Bledos and Enramadas oriented NW, 4) emplacement of Panalillo ignimbrite at 28 Ma filling the VRG and erupting from fissures related to the oblique grabens, and eruption of Placa basalt apparently also from fault-controlled vents.

  13. A new species of earth snake (Dipsadidae, Geophis) from Mexico

    Science.gov (United States)

    Canseco-Márquez, Luis; Pavón-Vázquez, Carlos J.; López-Luna, Marco Antonio; Nieto-Montes de Oca, Adrián

    2016-01-01

    Abstract A new species of the Geophis dubius group is described from the mountains of the Sierra Zongolica in west-central Veracruz and the Sierra de Quimixtlán in central-east Puebla. The new species is most similar to Geophis duellmani and Geophis turbidus, which are endemic to the mountains of northern Oaxaca and the Sierra Madre Oriental of Puebla and Hidalgo, respectively. However, the new species differs from Geophis duellmani by the presence of postocular and supraocular scales and from Geophis turbidus by having a bicolor dorsum. With the description of the new species, the species number in the genus increases to 50 and to 12 in the Geophis dubius group. Additionally, a key to the species of the Geophis dubius group is provided. PMID:27587979

  14. Effects of nematicides on nematode densities in turf in Connecticut.

    Science.gov (United States)

    Miller, P M

    1978-04-01

    The plant-parasitic nematodes Criconemoides lobatum, Hoplolaimus tylenchiformis, and Tylenchorhynchus dubius were present in the top 7.5 cm of sod consisting of numerous stolons and fibrous roots. Phenamiphos and 1,2-dibromo-3-chloropropane (DBCP) controlled all three species, whereas ethoprop and oxamyl controlled H. tylenchiformis and T. dubius but not C. lobatum. Benomyl and carbofuran controlled H. tylenchiformis but had poor control of C. lobatum and T. dubius. The effectiveness of carbofuran varied with the type of formulation, being most effective as a quick-release formulation. C. lobatum was the most difficult to control with chemicals. No chemical treatment improved the growth of 'Astoria colonial' bentgrass (A. tenuis Sibth.) or Kentucky bluegrass (Poa pratensis L.) under the moist condtions prevalent in these tests.

  15. A new species of earth snake (Dipsadidae, Geophis) from Mexico.

    Science.gov (United States)

    Canseco-Márquez, Luis; Pavón-Vázquez, Carlos J; López-Luna, Marco Antonio; Nieto-Montes de Oca, Adrián

    2016-01-01

    A new species of the Geophis dubius group is described from the mountains of the Sierra Zongolica in west-central Veracruz and the Sierra de Quimixtlán in central-east Puebla. The new species is most similar to Geophis duellmani and Geophis turbidus, which are endemic to the mountains of northern Oaxaca and the Sierra Madre Oriental of Puebla and Hidalgo, respectively. However, the new species differs from Geophis duellmani by the presence of postocular and supraocular scales and from Geophis turbidus by having a bicolor dorsum. With the description of the new species, the species number in the genus increases to 50 and to 12 in the Geophis dubius group. Additionally, a key to the species of the Geophis dubius group is provided.

  16. INFOINCLUSÃO EM PIRAÍ DIGITAL: EVIDÊNCIAS EMPÍRICAS A PARTIR DA TEORIA ATOR-REDE

    Directory of Open Access Journals (Sweden)

    Adonai Teles

    2012-08-01

    Full Text Available O artigo apresenta o status quo do programa Piraí Digital, com base na Teoria Ator-Rede (ANT e no modelo heurístico de inclusão digital (2iD. Numa primeira etapa desta pesquisa, o modelo heurístico original foi expandido com base nos pressupostos da ANT, gerando uma versão mais adequada (e2iD para a observação do fenômeno da infoinclusão em Piraí. Em seguida, a ANT foi adotada como suporte metodológico para que os autores, por meio de entrevistas e observação direta, compreendessem, sob a ótica dos atores ouvidos, como a infoinclusão tem ocorrido no município de Piraí. A partir daí, os resultados são apresentados com base na taxonomia da ANT e dos elementos do modelo e2ID. O presente trabalho de pesquisa sobre a infoinclusão em Piraí começou em 2007 e se estendeu até os últimos meses de 2009. Concluiu-se que a inclusão digital em Piraí tem sido uma realidade na administração pública local, na educação e na saúde. Porém, percebeu-se que o setor produtivo e a sociedade civil local, pouco ou nada tem se beneficiado do programa em questão. Finalmente, foi possível compreender o papel dos vários atores públicos nas redes de atores da ANT e explicar porque os resultados de Piraí Digital não têm, até agora, impactado homogeneamente a sociedade local.

  17. Control Químico de Malezas en Fríjol en el Valle de Medellín

    Directory of Open Access Journals (Sweden)

    Carmona B. Carlos

    1972-08-01

    Full Text Available En el C.N.I.A "Tulio Ospina", se realizaron durante tres semestres consecutivos, ensayos sobre control de malezas en el cultivo de fríjol. Las malezas que predominaron durante el experimento fueron: hoja angosta: coquito y argentina; de hoja ancha: falsa uchuva, bledo, verdolaga, botoncillo, y cadillo falso. En cuanto a los herbicidas que controlaron malezas y no causaron fitotoxicidad al fríjol se pueden mencionar: vernolale, a las dosis de 3.0 y 5.0 Kg. I.A./Ha., el cual fue bastante efectivo para el control de malezas de hoja angosta, pero, poco o nada efectivo para las malezas de hoja ancha. Metobromurón y C-6313, en la dosis de 2.0 Kg. I.A./Ha., presentaron buen control de todas las malezas, excepto el coquito. El DNBP a la dosis de 6.0 Kg. I.A./Ha., en sus dos forma (Premerge y Caldón presentó un buen control de toda las malezas, excepto el coquito y el bledo. Es importante mencionar el efecto de malezas que se consideran de segundo orden. Estas, una vez eliminadas las malezas primarias, se desarrollan con gran agresividad y pueden llegar a ocasionar pérdidas aún mayores. Esta situación se presentó principalmente en relación con el bledo y la falsa uchuva. De las anteriores conclusiones se deduce que para hacer recomendaciones es necesario tener muy en cuenta las malezas predominantes./Abstract Three different tests with herbicides on beans were made during 3 consecutive semesters in the C.N.I.A. Tulio Ospina in the Medellín valley. The weeds present were: coquito (Cyperus rotundus, pasto Argentina (Cynodon dactylon, falsa uchuva (Nycandra physalodes, bledo (Amaranthus sp., verdolaga (Portulaca oleracea, botoncillo (Galinsoga parviflora y cadillo falso (XanthiulIl occidentale. The herbicides tested and their doses were: C-63l3 (LO, 1.5 and 2.0 kg., A. I. /Ha.; Bensulide (4.0 and 6.0 kg. A.I. /Ha.; Vernolate (3.0, 5.0 and 6.0 kg. A. I. /Ha.; Fluometuron (2.0 kg. A. I. /Ha.; Trifluralina (1.5 and 2.0 kg. A. I. /Ha.; DNBP (P

  18. Reprodução do pirá-brasília, Simpsonichthys boitonei Carvalho (Cyprinodontiformes, Rivulidae, e caracterização de seu habitat na Reserva Ecológica do Instituto Brasileiro de Geografia e Estatística, Brasília, Distrito Federal, Brasil Reproduction of pira-brasília, Simpsonichthys boitonei Carvalho (Cyprinodontiformes, Rivulidae and its habitat characterization in the Reserva Ecológica do Instituto Brasileiro de Geografia e Estatística, Brasília, Distrito Federal, Brazil

    Directory of Open Access Journals (Sweden)

    Oscar A. Shibatta

    2005-12-01

    Full Text Available Este trabalho teve como objetivo analisar a reprodução e as características físicas e químicas do habitat de Simpsonichthys boitonei Carvalho, 1959, uma espécie rara e endêmica do Distrito Federal. Os peixes foram coletados com peneira em uma poça temporária localizada na Reserva Ecológica do Instituto Brasileiro de Geografia e Estatística (RECOR, bacia do rio Taquara, Distrito Federal. Os exemplares foram medidos (comprimento total em mm e pesados (peso total, peso das gônadas femininas e peso do corpo em gramas para o cálculo do fator de condição (K, do índice gonado-somático (IGS e do índice gonadal (IG. Também foram feitas análises físicas e químicas do ambiente. O brejo permaneceu com água durante oito meses (de dezembro a agosto, com transparência total, profundidade não ultrapassando 25 cm, condutividade menor que 15 µS/cm e pH entre 4,70 a 5,11. O oxigênio dissolvido variou entre 2,60 a 4,15 ppm, e a temperatura da água entre 20 a 25,5ºC. A análise da reprodução evidenciou que são peixes de desova parcelada, cujas fêmeas adultas permanecem com fator de condição alto durante boa parte da vida, diminuindo à medida que envelhecem. Os ovos foram depositados no substrato, e o período de incubação variou de 55 a 100 dias. Em laboratório esses ovos foram mantidos em água, onde 15,4% completaram o desenvolvimento embrionário. A proporção de fêmeas foi significativamente maior que a de machos. Pelas características acima, pode-se inferir que duas gerações do pirá-brasília podem ser encontradas no mesmo período de inundação.This study aims to analyze the reproduction and the physical and chemical characteristics of the habitat of Simpsonichthys boitonei Carvalho, 1959, a rare and endemic species of Distrito Federal. The fish were collected with sieve in a temporary pool located in the Reserva Ecológica do Instituto Brasileiro de Geografia e Estatística (RECOR, rio Taquara basin, Distrito Federal. The specimens were measured (total length in mm, weighted (total weight, feminine gonad weight and body weight in grams, for calculation of condition factor (K, gonad-somatic index (IGS and gonad index (IG. Physical and chemical analyses of environment were also made. The temporary pool was filled with water for eight months (December to August, with total transparency, depth not surpassing 25 cm, conductivity smaller than 15 µS/cm and pH among 4,70 to 5,11. The dissolved oxygen varied among 2,60 to 4,15 ppm, and the temperature of the water among 20 to 25,5 ºC. The analysis of reproduction evidenced that the fish has fractional spawning, whose adult females stay with high condition factor during great part of life, but decreasing with their age. The eggs were deposited in the substratum, and the incubation period varied among 55 to 100 days. In laboratory these eggs were maintained in water, where 15,4% complete the embryonic development. The proportion of females was significantly larger than males. For the characteristics above, we can infer that at least two generations of pirá-brasília can be found in the same flood period.

  19. Environmental Assessment: Military Housing Privatization Initiative Schriever Air Force Base, Colorado

    Science.gov (United States)

    2006-08-24

    tumble mustard (Sisymbrium altissimum), yellow sweetclover (Melilotus officina/is), and goatsbeard (Tragopogon dubius). There are no wetland...1 From USEPA 2004b, for batch mix plants using a natural gas-fired dryer , hot screens, and mixer. Table B-10. HAPs Emissions from Off-Site Hot Mix

  20. Application of the TerreSIM Model to a Training Area Landscape at Fort Bliss, TX

    Science.gov (United States)

    2005-09-01

    O.J., and S.C. Smith. 1991. “Responses to simulated leaf and root herbivory by a biennial, Tragopogon dubius,” Ecology 72:116-124. Risser , P.G., and...literature (Gigon and Rorison 1972; Barth and Klemmedson 1982; Gay et al. 1982; Nicholas and McGinnes 1982; Risser and Parton 1982; Vogt et al. 1982; Heil

  1. Study and Determination of As, Cr, and Pb in Amaranth Seeds

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    Elba Graciela Aguilar

    2013-01-01

    Full Text Available The concentration of As, Cr, and Pb toxic elements was determined in three species of amaranth seeds: A. hypochondriacus, A. cruentus, and A. dubius. The determinations were carried out by inductively coupled plasma optical atomic spectroscopy (ICP-OES. The result shows that Cr and As were found in minor concentrations than allowed by the World Health Organization (WHO; 120 and 4 mg kg−1 resp.; As (mg kg−1: A. dubius (0.76 A. cruentus (<0.50 and A. hypochondriacus (<0.50; Cr (mg kg−1: A. dubius (8.19 A. cruentus (1.15 and A. hypochondriacus (1.20. However, the concentration of Pb was high: A. dubius (19.04 A. cruentus (30.20 and A. hypochondriacus (35.56 more than the maximum of WHO (10 mg kg−1. To avoid systematic error, recovery and validation studies were performed: recovery test: 102.3%; validation (by standard addition: 96.0–103.1%. Due to the fact that amaranth had been proposed as new food due to its nutraceutical properties, the high concentration of Pb found in this study indicates that Pb should be evaluated in these amaranth species to avoid the intake of toxic element by human beings.

  2. Disease: H01159 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H01159 Anterior segment dysgenesis (ASD), including: Anterior segment mesenchymal d...ysgenesis; Aniridia; Axenfeld-Rieger syndrome (ARS); Peters anomaly; Iridogoniodysgenesis (IRID) Anterior se...OMIM: 107250 106210 180500 602482 604229 601631 137600 261540 PMID:18989383 Summers KM, Withers SJ, Gole GA, Piras S, Taylor PJ Anter...ior segment mesenchymal dysgenesis in a large Australian family is associated with

  3. A New Typology for State-Sponsored International Terrorism

    Science.gov (United States)

    2011-12-01

    Organization NIF National Islamic Front PIJ Palestinian Islamic Jihad PIRA Provisional Irish Republican Army WMD Weapons of Mass Destruction xii THIS PAGE...Islamic Front ( NIF ) to power. The party’s leader, Hassan al-Turabi, sought to establish an Islamist state in Sudan and offered sanctuary for Islamic

  4. Understanding Why Terrorist Operations Succeed or Fail

    Science.gov (United States)

    2009-01-01

    they can draw when deciding how many members are devoted to a particular attack.13 Large groups (e.g., PIRA, Hezbollah, or the Liberation Tigers of...the food supply: The case of botulinum toxin in milk ,” Proceedings of the National Academy of Sciences, Vol. 102, No. 28, 2005, pp. 9984–9989.

  5. Primer-introduced restriction analysis polymerase chain reaction method for non-invasive prenatal testing of β-thalassemia.

    Science.gov (United States)

    Liu, Saijun; Chen, Liyuan; Zhang, Xiandong; Li, Jian; Lin, Haiying; Liu, Louhui; Xie, Jiansheng; Ge, Huijuan; Ye, Minglan; Chen, Caifen; Ji, Xingwen; Zhang, Caifen; Xu, Fengping; Jiang, Hui; Zhen, Hefu; Chen, Shiping; Wang, Wei

    2015-01-01

    We have developed a new method for non-invasive prenatal testing (NIPT) of paternally inherited fetal mutants for β-thalassemia (β-thal). Specially designed primer-introduced restriction analysis-polymerase chain reaction (PIRA-PCR) were used to detect four major mutations [IVS-II-654, HBB: c.316-197C > T; codon 17 (A > T), HBB: c.52A > T; -28 (A > G), HBB: c.-78A > G and codons 41/42 (-TTCT), HBB: c.126_129delCTTT] causing β-thal in China. The PIRA-PCR assay was first tested in a series of mixed DNA with different concentrations and mixed proportions. Subsequently, this assay was further tested in 10 plasma DNA samples collected from pregnant women. In the DNA mixture simulation test, the PIRA-PCR assay was able to detect 3.0% target genomic DNA (gDNA) mixed in 97.0% wild-type gDNA isolated from whole blood. For plasma DNA testing, the results detected by PIRA-PCR assay achieved 100.0% consistency with those obtained from the amniocentesis analysis. This new method could potentially be used for NIPT of paternally inherited fetal mutants for β-thal.

  6. NCBI nr-aa BLAST: CBRC-HSAP-06-0001 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-06-0001 sp|Q01456|OAM_ASCSU Ovarian abundant message protein (Protein OAM...) pir||A48461 ovarian abundant message protein oam - common roundworm gb|AAA73355.1| [Ascaris lumbricoides mRNA sequence.], gene product Q01456 2e-11 52% ...

  7. NCBI nr-aa BLAST: CBRC-HSAP-02-0005 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-02-0005 sp|Q01456|OAM_ASCSU Ovarian abundant message protein (Protein OAM...) pir||A48461 ovarian abundant message protein oam - common roundworm gb|AAA73355.1| [Ascaris lumbricoides mRNA sequence.], gene product Q01456 2e-06 28% ...

  8. NCBI nr-aa BLAST: CBRC-GACU-10-0021 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GACU-10-0021 ref|YP_177714.1| PPE FAMILY PROTEIN [Mycobacterium tuberculosis H...37Rv] pir||A70524 probable PPE protein - Mycobacterium tuberculosis (strain H37RV) emb|CAE55269.1| PPE FAMILY PROTEIN [Mycobacterium tuberculosis H37Rv] YP_177714.1 1e-30 41% ...

  9. Micropropagação de cebola a partir de bulbinhos induzidos in vitro Micropropagation of onion from in vitro induced bulblet

    Directory of Open Access Journals (Sweden)

    Benedita Maria Rodrigues

    1996-01-01

    Full Text Available Visando determinar um método para micropropagação a partir de bulbinhos in vitro, de cultivares de cebola (Allium cepa L., foram realizados três experimentos, avaliando-se a influência do 6-benzilaminopurina (BAP x ácido naftalenoacético (ANA e posição do explante no meio de cultura, a influência de bulbinhos desenvolvidos em diversas concentrações de sacarose e o efeito do diâmetro dos bulbinhos sobre a taxa de multiplicação in vitro. Pelos resultados, a posição vertical dos explantes mostrou maior tendência para porcentagem de regeneração nos cultivares Pira Ouro e Pirana Precoce. 0 efeito das concentrações de fitorreguladores na porcentagem de regeneração não foi verificado para o 'Pira Ouro', enquanto para o 'Pirana Precoce', porcentagem mais elevada foi obtida em 4,0 mg/L de BAP x 0,5 mg/L de ANA. Maior taxa de multiplicação foi observada para os dois cultivares em BAP (2,0 mg/L x ANA (0,25 mg/L. Bulbinhos do cultivar Pira Ouro e híbrido entre Pira Ouro e Pirana Precoce, desenvolvidos em 120 g/L de sacarose, mostraram-se mais adequados à micropropagação in vitro no material utilizado. Com relação ao diâmetro inicial do explante, o 'Pira Ouro' não mostrou diferença estatística para os parâmetros avaliados, enquanto no 'Pirana Precoce' maior porcentagem de regeneração foi obtida nos diâmetros M (5 a 10 mm e P (Series of three different experiments were carried out, in order to study the optimization of the use of bulblets obtained in vitro, in the micropropagation of the onion (Allium cepa L.. Initially, it was evaluated the effects of the interaction between two doais of 6-benzylaminopurine (BAP and naphthaleneacetic acid (NAA, and the position of the explant in the medium, for the cultivara Pira Ouro and Pirana Precoce. For 'Pira Ouro' the phytohormone concentrations did not affect the percentage of regeneration; however, for 'Pirana Precoce', the rates of 4.0 mg/L of BAP and 0.5 mg/L of NAA gave the

  10. Potencial alelopático de Brassica campestris subsp. rapa y Lolium temulentum sobre tres especies de malezas de la Sabana de Bogotá

    Directory of Open Access Journals (Sweden)

    Carolina Zamorano

    2005-07-01

    Full Text Available Se realizaron bioensayos con el objeto de evaluar el potencial alelopático de los extractos de hojas y residuos de nabo silvestre (Brassica campestris subsp. rapa [L.] Hook. f. y raigrás (Lolium temulentum L. sobre tres especies de malezas de la Sabana de Bogotá: cenizo (Chenopodium petiolare Kunth, malva blanca (Fuertesimalva limensis [L.] Fryxell y bledo (Amaranthus hybridus L.. Los bioensayos en el laboratorio se desarrollaron con la técnica de plántulas en solución nutritiva, bajo un diseño completamente al azar con tres repeticiones y tres réplicas en el tiempo, y en invernadero, donde se usó mezcla de arena y turba (3:2 como sustrato y semillas pregerminadas. Los resultados obtenidos en laboratorio mostraron diferencias en los síntomas observados entre las diferentes especies, mientras que la variable peso fresco fue la que mejor describió el efecto de las concentración para los extractos de nabo, con una concentración que reduce la variable de respuesta en 50% (DC50 de 5,53 g· L-1 para bledo, 2,58 g· L-1 para cenizo y 7,72 g· L-1 para malva blanca. En el caso de raigrás, el peso fresco permitió el ajuste de una curva concentración-respuesta, con el fin de calcular la DC50. La respuesta entre las especies de malezas fue diferente respecto a la actividad de los extractos y de los residuos vegetales en suelo. En el caso del bledo, no se registraron diferencias entre los residuos en suelo y los extractos de nabo, mientras que con cenizo y malva blanca no hubo emergencia de plántulas bajo la condición de residuos en suelo de nabo. El peso fresco de plántulas de tomate disminuyó en cerca del 25% al crecer en residuos de nabo (6 ó 12 t· ha-1 de materia fresca y en cerca del 60% bajo residuos de raigrás (6 t· ha-1 de materia fresca.

  11. Características qualitativas de cultivares de cebola no sul de Minas Gerais Qualitative characteristics of onion cultivars in southern Minas Gerais State

    Directory of Open Access Journals (Sweden)

    Silvio Júlio de Rezende Chagas

    2004-02-01

    Full Text Available Com o objetivo de determinar a composição físico-química e química de cultivares de cebola (Allium cepa L., conduziu-se um ensaio na Fazenda Experimental de Lavras da EPAMIG, Lavras-MG, no período de março a setembro de 1995. O delineamento experimental foi de blocos ao acaso com quatro repetições e 6 tratamentos, constituídos pelas cultivares Baia Periforme, Crioula, Granex 33, Pira Ouro, Jubileu e Texas Grano 502. Após a cura, avaliaram-se o pH, sólidos solúveis e totais (%, acidez titulável (%, ácido pirúvico (µmol/g, açúcares totais, redutores e não-redutores (% e índice industrial dos bulbos. As cultivares Crioula, Baia Periforme, Pira Ouro e Jubileu apresentaram as maiores concentrações de sólidos totais e solúveis. A acidez mais elevada foi observada nas cultivares Crioula (0,370% e Pira Ouro (0,315%. Os maiores índices industriais foram apresentados pelas cultivares Crioula (0,87 e Baia Periforme (0,75, e os menores, pelas cultivares Texas Grano 502 (0,51 e Granex 33 (0,32.This study was carried out from March to September of 1995, in the Experimental Field of EPAMIG, Lavras-MG, Brazil, with the objective of determining the physico-chemical and chemical composition of onions from different cultivars (Allium cepa L.. The experimental design was completely randomized blocks with four repetitions and six cultivars as follow: Baia Periforme, Crioula, Granex 33, Pira Ouro, Jubileu and Texas Grano 502. After the cure it was evaluated the pH, soluble and total solids (%, titrate acidity (%, pyruvic acid (µmol/g, total sugar, reducers and non reducers (% and industrial index. The cultivars Crioula, Baia Periforme, Pira Ouro and Jubileu showed the largest concentrations of total and soluble solids. The highest acidity indexes were observed in the cultivars Crioula (0.370% and Pira Ouro (0.315%. The largest industrial indexes were found in the cultivars Crioula (0.87 and Baia Periforme (0.75, and the smallest ones in the

  12. Redescripción de una especie y descripción de dos especies nuevas de Acanthococcus Signoret (Hemiptera, Eriococcidae de la región Neotropical Redescription of one species and description of two new species of Acanthococcus Signoret (Hemiptera, Eriococcidae from the Neotropical Region

    Directory of Open Access Journals (Sweden)

    Patricia González

    2011-06-01

    Full Text Available Se redescriben e ilustran la hembra adulta y la ninfa del primer estadio de Acanthococcus piptadeniae (Hempel, de Brasil y se describen e ilustran dos especies nuevas de la Argentina basado en la hembra adulta. Se presenta una clave para las especies de Acanthococcus con seis setas en las protibias y cinco en meso y metatibias, incluyendo a Acanthococcus dubius (Cockerell, citada para la región Neotropical.The adult female and the first-instar nymph of Acanthococcus piptadeniae (Hempel, from Brazil, are redescribed and illustrated, and two new species from Argentina are described and illustrated based on the adult female. A key to Acanthococcus species with six setae on the protibiae and five on meso- and metatibiae, including Acanthococcus dubius (Cockerell, cited in the Neotropical Region, is presented.

  13. The structure of umkomasiacean fructifications from the Triassic of Queensland

    Directory of Open Access Journals (Sweden)

    Pattemore Gary A.

    2016-06-01

    Full Text Available The plant fossil genera Umkomasia Thomas 1933 and Pteruchus Thomas 1933 emend. Townrow 1962 are known chiefly from the Middle and Upper Triassic of Gondwana. The structure of these fructifications has been conjectural, some being identified as pinnate, others as helically arranged. Specimens from the Ladinian-lower Norian of Queensland (northeastern Australia show that the female and male fructifications - U. geminata (Shirley 1898 Rigby in Playford et al. 1982 emend. nov. and P. dubius Thomas 1933 emend. Townrow 1962, respectively - have a bipinnate structure. Those fructifications and the bipinnate leaf, Dicroidium feistmantelii (Johnston 1894 Gothan 1912, probably all belonged to the same parent plant. It was first suggested by John Townrow in 1962 that the sporangial heads of P. dubius have a pinnate structure; this character is confirmed herein. Pteruchus is recorded for the first time from the Carnian Tarong Basin, Queensland.

  14. Decoding Different Patterns in Various Grey Tones Incorporated in the QR Code

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    Filip Cvitić

    2014-07-01

    Full Text Available Using colors in bar codes causes errors that may adversely affect their readability (Tan etal. 2010, given that the contrast between data and background modules is reduced. Due to the unreliability of using color bar codes, most designers still keep to the limitations placed by Pira International (Smithers Pira in 2002 (Williams, 2004. Since the contrast between data modules and background modules is the most important aspect in the process of reliable bar code decoding, this paper explores the dependence of reliable decoding of QR codes incorporated with combinations of grey tones on the technical characteristics of the cameras on smartphones that were marketed in the period between 2008 and 2012.

  15. Redescripción de una especie y descripción de dos especies nuevas de Acanthococcus Signoret (Hemiptera, Eriococcidae de la región Neotropical

    Directory of Open Access Journals (Sweden)

    Patricia González

    2011-06-01

    Full Text Available Se redescriben e ilustran la hembra adulta y la ninfa del primer estadio de Acanthococcus piptadeniae (Hempel, de Brasil y se describen e ilustran dos especies nuevas de la Argentina basado en la hembra adulta. Se presenta una clave para las especies de Acanthococcus con seis setas en las protibias y cinco en meso y metatibias, incluyendo a Acanthococcus dubius (Cockerell, citada para la región Neotropical.

  16. Approaches Towards the Identification of Patterns in Violent Events, Baghdad, Iraq

    Science.gov (United States)

    2009-05-01

    Gianfranco Piras May 2009 C on st ru ct io n E n gi n ee ri n g R es ea rc h L ab or at or y Approved for public release; distribution is... GIS operations. Julia Koschinsky provided logistical support. The report and its findings are those of the authors alone, and should not be attributed...news sources. While precise geocoding was not possible, there was sufficient information in the reports to associate each event with a neighborhood. A

  17. Killing Range

    OpenAIRE

    Asal, Victor; Gill, Paul; Rethemeyer, R. Karl; Horgan, John

    2015-01-01

    This paper presents an analysis of the Provisional Irish Republican Army's (PIRA) brigade level behavior during the Northern Ireland Conflict (1970-1998) and identifies the organizational factors that impact a brigade's lethality as measured via terrorist attacks. Key independent variables include levels of technical expertise, cadre age, counter-terrorism policies experienced, brigade size, and IED components and delivery methods. We find that technical expertise within a brigade allows for ...

  18. Dicty_cDB: VSD680 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available nces producing significant alignments: (bits) Value VSD680 (VSD680Q) /CSM/VS/VSD6-D/VSD680Q.Seq.d/ 228 1e-59...ology vs DNA Score E Sequences producing significant alignments: (bits) Value N (...quences producing significant alignments: (bits) Value (Q6UK63) RecName: Full=Protein pirA; &AY368270_1(AY36

  19. NCBI nr-aa BLAST: CBRC-OCUN-01-0782 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available pira borgpetersenii serovar Hardjo-bovis L550] ref|YP_801264.1| Prolipoprotein diacylglyceryltransferase [Le...ptospira borgpetersenii serovar Hardjo-bovis JB197] gb|ABJ78582.1| Prolipoprotein diacylglyceryltransferase ...[Leptospira borgpetersenii serovar Hardjo-bovis L550] gb|ABJ76506.1| Prolipoprote...in diacylglyceryltransferase [Leptospira borgpetersenii serovar Hardjo-bovis JB197] YP_797515.1 3.3 35% ...

  20. NCBI nr-aa BLAST: CBRC-GGAL-35-0050 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGAL-35-0050 ref|YP_177980.1| PE-PGRS FAMILY PROTEIN [Mycobacterium tuberculosis... H37Rv] pir||A70807 hypothetical glycine-rich protein Rv3511 - Mycobacterium tuberculosis (strain H37RV) e...mb|CAE55605.1| PE-PGRS FAMILY PROTEIN [Mycobacterium tuberculosis H37Rv] YP_177980.1 2e-18 33% ...

  1. NCBI nr-aa BLAST: CBRC-XTRO-01-2365 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-2365 ref|YP_177980.1| PE-PGRS FAMILY PROTEIN [Mycobacterium tuberculosis... H37Rv] pir||A70807 hypothetical glycine-rich protein Rv3511 - Mycobacterium tuberculosis (strain H37RV) e...mb|CAE55605.1| PE-PGRS FAMILY PROTEIN [Mycobacterium tuberculosis H37Rv] YP_177980.1 0.17 36% ...

  2. Killing Range: Explaining Lethality Variance within a Terrorist Organization.

    Science.gov (United States)

    Asal, Victor; Gill, Paul; Rethemeyer, R Karl; Horgan, John

    2015-04-01

    This paper presents an analysis of the Provisional Irish Republican Army's (PIRA) brigade level behavior during the Northern Ireland Conflict (1970-1998) and identifies the organizational factors that impact a brigade's lethality as measured via terrorist attacks. Key independent variables include levels of technical expertise, cadre age, counter-terrorism policies experienced, brigade size, and IED components and delivery methods. We find that technical expertise within a brigade allows for careful IED usage, which significantly minimizes civilian casualties (a specific strategic goal of PIRA) while increasing the ability to kill more high value targets with IEDs. Lethal counter-terrorism events also significantly affect a brigade's likelihood of killing both civilians and high-value targets but in different ways. Killing PIRA members significantly decreases IED fatalities but also significantly decreases the possibility of zero civilian IED-related deaths in a given year. Killing innocent Catholics in a Brigade's county significantly increases total and civilian IED fatalities. Together the results suggest the necessity to analyze dynamic situational variables that impact terrorist group behavior at the sub-unit level.

  3. Análise sensorial, teores de nitrato e de nutrientes de alface cultivada em hidroponia sob águas salinas Sensory analysis, nitrate and nutrient concentration of lettuce grown in hydroponics under saline water

    Directory of Open Access Journals (Sweden)

    Dalva Paulus

    2012-03-01

    Full Text Available O presente trabalho teve como objetivos determinar os teores de nutrientes e de nitrato e realizar análise sensorial de alface cultivada em sistema hidropônico sob água salina. O experimento foi conduzido em ambiente protegido no período de dezembro de 2007 a janeiro de 2008, em Piracicaba (SP. O delineamento experimental foi de blocos ao acaso, sendo estudados cinco níveis de salinidade da água de irrigação, utilizando-se NaCl (Condutividade elétrica da água (CEa: 0,42, 1,53, 3,52, 5,55 e 7,43 dS m-1 em duas cultivares de alface (Verônica e Pira Roxa, em esquema fatorial. Foram avaliados a análise sensorial aplicando escala hedônica e através dessa, o que os provadores mais apreciaram e o que menos gostaram de cada amostra, intenção de compra e consumo, teor de nitrato, absorção de nutrientes pela folhas e determinação dos nutrientes presentes na solução nutritiva. Para o atributo sabor a cultivar Verônica recebeu as melhores notas no nível de salinidade 1,53 e 5,55 dS m-1. Para a cultivar Pira Roxa a melhor nota foi atribuída para a alface produzida na água não salina. Para os demais atributos não houve diferença significativa nos diferentes níveis de salinidade. Com relação à intenção de compra, constatou-se que as alfaces cv. Verônica e Pira Roxa apresentaram boa aceitação de mercado. Os menores níveis de nitrato (1960 mg kg-1 e 2620 mg kg-1 de massa de matéria fresca, da Verônica e Pira Roxa, respectivamente, foram relacionados à condutividade elétrica de 0,42 dS m-1, aumentando a salinidade da água para 7,43 dS m-1, o teor foliar de nitrato aumentou para 2500 mg kg-1 e 3420 mg kg-1 para as cultivares Verônica e Pira Roxa. Como o tempo de exposição da alface à salinidade em sistema hidropônico foi curto, em torno de 25 dias, não se verificou sintomas de deficiência nutricional e toda alface foi classificada como apropriada para consumo humano.This study aimed to evaluate the content of

  4. Earthworm assemblages in different intensity of agricultural uses and their relation to edaphic variables

    Directory of Open Access Journals (Sweden)

    LB Falco

    2015-05-01

    Full Text Available The objective of this study was to relate earthworm assemblage structure with three different soil use intensities, and to indentify the physical, chemical, and microbiological soil variables that are associated to the observed differences. Three soil uses were evaluated: 1-Fifty year old naturalized grasslands, low use intensity; 2-Recent agricultural fields, intermediate use intensity, and 3-Fifty year old intensive agricultural fields, high use intensity. Three different sites for each soil use were evaluated from winter 2008 through summer 2011. Nine earthworm species were identified across all sampling sites. The sites shared five species: the native Microscolex dubius, and the introduced Aporrectodea caliginosa, A. rosea, Octalasion cyaneum, and O. lacteum, but they differed in relative abundance by soil use. The results show that the earthworm community structure is linked to and modulated by soil properties. Both species abundance and diversity showed significant differences depending on soil use intensity. A principal component analysis showed that species composition is closely related to the environmental variability. The ratio of native to exotic species was significantly lower in the intensive agricultural system when compared to the other two, lower disturbance systems. Microscolex dubius abundance was related to naturalized grasslands along with soil Ca, pH, mechanical resistance, and microbial respiration. Aporrectodea caliginosa abundance was related to high K levels, low enzymatic activity, slightly low pH, low Ca, and appeared related to the highly disturbed environment. Eukerria stagnalis and Aporrectodea rosea, commonly found in the recent agricultural system, were related to high soil moisture condition, low pH, low Ca and low enzymatic activity. These results show that earthworm assemblages can be good indicators of soil use intensities. In particular, Microscolex dubius, Aporrectodea caliginosa, and Aporrectodea rosea

  5. Earthworm assemblages in different intensity of agricultural uses and their relation to edaphic variables.

    Science.gov (United States)

    Falco, L B; Sandler, R; Momo, F; Di Ciocco, C; Saravia, L; Coviella, C

    2015-01-01

    The objective of this study was to relate earthworm assemblage structure with three different soil use intensities, and to indentify the physical, chemical, and microbiological soil variables that are associated to the observed differences. Three soil uses were evaluated: 1-Fifty year old naturalized grasslands, low use intensity; 2-Recent agricultural fields, intermediate use intensity, and 3-Fifty year old intensive agricultural fields, high use intensity. Three different sites for each soil use were evaluated from winter 2008 through summer 2011. Nine earthworm species were identified across all sampling sites. The sites shared five species: the native Microscolex dubius, and the introduced Aporrectodea caliginosa, A. rosea, Octalasion cyaneum, and O. lacteum, but they differed in relative abundance by soil use. The results show that the earthworm community structure is linked to and modulated by soil properties. Both species abundance and diversity showed significant differences depending on soil use intensity. A principal component analysis showed that species composition is closely related to the environmental variability. The ratio of native to exotic species was significantly lower in the intensive agricultural system when compared to the other two, lower disturbance systems. Microscolex dubius abundance was related to naturalized grasslands along with soil Ca, pH, mechanical resistance, and microbial respiration. Aporrectodea caliginosa abundance was related to high K levels, low enzymatic activity, slightly low pH, low Ca, and appeared related to the highly disturbed environment. Eukerria stagnalis and Aporrectodea rosea, commonly found in the recent agricultural system, were related to high soil moisture condition, low pH, low Ca and low enzymatic activity. These results show that earthworm assemblages can be good indicators of soil use intensities. In particular, Microscolex dubius, Aporrectodea caliginosa, and Aporrectodea rosea, showed different

  6. PIR-B-deficient mice are susceptible to Salmonella infection.

    Science.gov (United States)

    Torii, Ikuko; Oka, Satoshi; Hotomi, Muneki; Benjamin, William H; Takai, Toshiyuki; Kearney, John F; Briles, David E; Kubagawa, Hiromi

    2008-09-15

    Paired Ig-like receptors of activating (PIR-A) and inhibitory (PIR-B) isoforms are expressed by many hematopoietic cells, including B lymphocytes and myeloid cells. To determine the functional roles of PIR-A and PIR-B in primary bacterial infection, PIR-B-deficient (PIR-B(-/-)) and wild-type (WT) control mice were injected i.v. with an attenuated strain of Salmonella enterica Typhimurium (WB335). PIR-B(-/-) mice were found to be more susceptible to Salmonella infection than WT mice, as evidenced by high mortality rate, high bacterial loads in the liver and spleen, and a failure to clear bacteria from the circulation. Although blood levels of major cytokines and Salmonella-specific Abs were mostly comparable in the two groups of mice, distinct patterns of inflammatory lesions were found in their livers at 7-14 days postinfection: diffuse spreading along the sinusoids in PIR-B(-/-) mice vs nodular restricted localization in WT mice. PIR-B(-/-) mice have more inflammatory cells in the liver but fewer B cells and CD8(+) T cells in the spleen than WT mice at 14 days postinfection. PIR-B(-/-) bone marrow-derived macrophages (BMMphi) failed to control intracellular replication of Salmonella in vitro, in part due to inefficient phagosomal oxidant production, when compared with WT BMMphi. PIR-B(-/-) BMMphi also produced more nitrite and TNF-alpha upon exposure to Salmonella than WT BMMphi did. These findings suggest that the disruption of PIR-A and PIR-B balance affects their regulatory roles in host defense to bacterial infection.

  7. Dual Effects of IL-1 Overactivity on the Immune System in a Mouse Model of Arthritis due to Deficiency of IL-1 Receptor Antagonist

    Institute of Scientific and Technical Information of China (English)

    Jian Yan; Yan Jiao; Hong Chen; Feng Jiao; Karen A.Hasty; John M.Stuart; Weikuan Gu

    2013-01-01

    Previous studies have revealed the significance of cytokine interleukin 1 (IL-1) in the onset and progression of meumatoid arthritis (RA).The precise molecular mechanisms related to IL-1 underlying RA is still elusive.We conducted a whole genome-wide transcriptomal comparison of wild-type (WT) and arthritis-prone IL-1 receptor antagonist (IL-Irn) deficient BALB/c mice to address this issue.To refine our search efforts,gene expression profiling was also performed on paired wild-type and arthritis-resis nt IL-1m deficient DBA/1 mice as internal controls when identifying causative arthritis candidate genes.Two hundred and fifteen trans dpts were found to be dysregulated greater than or equal to 2-fold in the diseased mice.The altered transcriptome in BALB/c mice revealed increased myeloid cell activities and impaired lymphocyte functionality,suggesting dual regulatory effects of IL-1 hyperactivil on immunological changes associated with arthritis development.Phase-specific gene expression changes were identified,such as early increase and late decrease of heat shock protein coding genes.Moreover,common gene expression changes were also observed,especially the upregulation of paired Ig-like receptor A (Pira) in both early and late phases of arthritis.Real-time PCR was performed to validate the expression of Pira and an intervention experiment with a major histocompatibility complex (MHC) class I inhibitor (brefeldin A) was carried out to investigate the role of suppressing Pira activity.We conclude that global pattern changes of common and distinct gene expressions may represent novel opportunities for better control of RA through early diagnosis and development of alternative therapeutic strategies.

  8. Ácido giberélico, bissulfureto de carbono e ácido 2-4 cloroetil fosfônico e a dormência e produtividade de tubérculos de batata Giberellic acid, carbon-disulphide and 2-4 chloro-ethyl phosphonic and the dormancy and yield of potato tubers

    Directory of Open Access Journals (Sweden)

    Ricardo Antonio Ayub

    1999-01-01

    Full Text Available Com o objetivo de avaliar a eficiência do ácido giberélico, bissulfureto de carbono e ácido 2-4 cloroetil-fosfônico (EthrellR na quebra de dormência e na produção de batata, cv. "Marijke", foi conduzido um ensaio no período de 1990/ 91 em Piraí do Sul, PR . A superioridade do tratamento com o ácido 2-4-cloroetil-fosfônico em relação ao ácido giberélico e ao bissulfureto de carbono, aliada a sua facilidade de aplicação, justifica a sua recomendação com vistas a quebra de dormência de tubérculos do genótipo de batata em estudo, para a região de Piraí do Sul, PR, onde a dose de 842 mg.L-1 de EthrellR ocasionou a produção máxima de 23395 kg/ha.In order to evaluate the efficiency of giberellic acid, carbon-disulphide, and 2-4 chloro-ethyl phosphonic acid (EthrellR on dormancy breaking and yield of potato cv. 'Marijke', an essay was conducted at Piraí do Sul, PR, Brazil, during 1990/1991. The superiority of the EthrellR treatment over giberellic acid and carbon-disulphide, and its easy application, justify its use for dormancy breaking of potato tubers, for the studied genotype and location, at the rate of 842 mg.L-1, resulting in a potato yield of 23,395 kg/ha.

  9. Construct Space Innovation and Development Center (SID) at Schriever Air Force Base, Colorado

    Science.gov (United States)

    2006-03-01

    Russian thistle Salsola iberica 2,382.7 B Goatsbeard Tragopogon dubius 59.0 B Tumble mustard Sisymbrium altissimum 34.9 B Kochia Kochia scorpia 38.4 C...mix asphalt Emission factors are for batch mix plants using a natural gas fired dryer , hot screens, and mixer Emission factors are from AP-42 Vol I...plants using a natural gas fired dryer , hot screens, and mixer Emission factors are from AP-42 Vol I Chapter 11.1 Hot Mix Asphalt Plants, April 2004

  10. Environmental Assessment: Space Innovation and Development Center Schriever AFB, Colorado

    Science.gov (United States)

    2006-03-01

    officinalis 2,023.7 B Russian thistle Salsola iberica 2,382.7 B Goatsbeard Tragopogon dubius 59.0 B Tumble mustard Sisymbrium altissimum 34.9 B Kochia...HMA = hot mix asphalt Emission factors are for batch mix plants using a natural gas fired dryer , hot screens, and mixer Emission factors...are for batch mix plants using a natural gas fired dryer , hot screens, and mixer Emission factors are from AP-42 Vol I Chapter 11.1 Hot Mix Asphalt

  11. SOME ASPECTS OF THE NATURAL CONTROL OF PLANT PARASITIC NEMATODES IN SOIL UNDER BROAD BEAN VICIA FABA L. CULTIVATED IN CROP ROTATION AND LONG-TERM MONOCULTURE.

    Science.gov (United States)

    Skwiercz, A T; Damszel, M; Stefanovska, T; Rychcik, B

    2015-01-01

    Observations on population density of plant parasitic nematodes occurring in rhizosphere of broad bean cultivated in the crop rotation and long-term monoculture were performed during 2013-2014. 13 species were observed: Trichodorus primitivus, T. viruliferus, Paratrichodorus pachydermus, Criconema annuliferum, Paratylenchus projectus, Bitylenchus dubius, Merlinius brevidens, Pratylenchus fallax, P. flakkensis, P. neglectus, Heterodera triffolii, H. goettingiana, and Ditylenchus dipsaci. In monoculture plots 70-80% of eggs inside Heterodera cysts were colonized by pathogenic fungi (v.s. 50-62% of cysts from crop rotation). 12-18% of specimens of Pratylenchus species were colonized by the nematode-pathogenic bacteria: Bacillus penetrans.

  12. The Pasteur effect in rat jejunum and the influence of nematode infections.

    Science.gov (United States)

    Scofield, A M

    1978-01-01

    1. The Pasteur effect was shown after 15 min but not after 30 min incubation of jejunal rings from normal rats. 2. During 15-30 min incubation, the rate of anaerobic lactate production decreased, while aerobic lactate production remained unchanged. Thus oxygen was necessary to maintain the functional integrity of the tissue during this period. 3. After infection with either Nematospiroides dubius or Nippostrongylus brasiliensis, the Pasteur effect could not usually be shown, mainly due to a reduced rate of anaerobic lactate production. 4. The possible relationship of the loss of the Pasteur effect to the immune response is discussed.

  13. NCBI nr-aa BLAST: CBRC-DNOV-01-1085 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-1085 ref|NP_240139.1| high-affinity zinc uptake system membrane protein Zn...uB [Buchnera aphidicola str. APS (Acyrthosiphon pisum)] sp|P57402|ZNUB_BUCAI High-affinity zinc uptake s...ystem membrane protein znuB pir||A84967 hypothetical protein znuB [imported] - Buchnera sp. (strain APS) dbj...|BAB13025.1| high-affinity zinc uptake system membrane protein ZnuB [Buchnera aphidicola str. APS (Acyrthosiphon pisum)] NP_240139.1 0.53 36% ...

  14. Uso da taxocenose de peixes como indicadora de degradação ambiental no rio Paraíba do Sul, Rio de Janeiro, Brasil

    OpenAIRE

    Francisco Gerson Araújo

    1998-01-01

    An adaptation of index of biotic integrity (IBI) was performed for the first time in Brazil at the Rio Paraíba do Sul, between Barra Mansa and Barra do Piraí, a very important extent of this river because of the high pollution levels, and for being the main abstraction source of Rio de Janeiro city water supply. A monthly standardised sampling programme, from March-1995 to February-1996, was carried out at four sites. Seines, casting nets and net trays were used for catching fishes. IBI was o...

  15. Monogenoidea en Serrasalmus rhombeus (Linnaeus, 1766) de la Cuenca Amazónica Boliviana

    OpenAIRE

    Leslie Córdova; Antoine Pariselle

    2013-01-01

    El presente trabajo analiza la diversidad de parásitos monogenoideos como un carácter indepen- diente, esperando que por su alta especifi cidad, éstos parásitos puedan caracterizar las poblaciones de sus hospederos, la piraña blanca, Serrasalmus rhombeus . El estudio se llevó acabo en cuatro sub-cuencas de la Cuenca Amazónica Boliviana (Iténez, Beni, Madre de Dios e Ichilo); se obtuvie- ron 1135 parásitos, los cuales fueron agrupados en seis géneros y once especies: Anacanthorus jegui, A. ama...

  16. On the road to diploidization? Homoeolog loss in independently formed populations of the allopolyploid Tragopogon miscellus (Asteraceae

    Directory of Open Access Journals (Sweden)

    Soltis Pamela S

    2009-06-01

    Full Text Available Abstract Background Polyploidy (whole-genome duplication is an important speciation mechanism, particularly in plants. Gene loss, silencing, and the formation of novel gene complexes are some of the consequences that the new polyploid genome may experience. Despite the recurrent nature of polyploidy, little is known about the genomic outcome of independent polyploidization events. Here, we analyze the fate of genes duplicated by polyploidy (homoeologs in multiple individuals from ten natural populations of Tragopogon miscellus (Asteraceae, all of which formed independently from T. dubius and T. pratensis less than 80 years ago. Results Of the 13 loci analyzed in 84 T. miscellus individuals, 11 showed loss of at least one parental homoeolog in the young allopolyploids. Two loci were retained in duplicate for all polyploid individuals included in this study. Nearly half (48% of the individuals examined lost a homoeolog of at least one locus, with several individuals showing loss at more than one locus. Patterns of loss were stochastic among individuals from the independently formed populations, except that the T. dubius copy was lost twice as often as T. pratensis. Conclusion This study represents the most extensive survey of the fate of genes duplicated by allopolyploidy in individuals from natural populations. Our results indicate that the road to genome downsizing and ultimate genetic diploidization may occur quickly through homoeolog loss, but with some genes consistently maintained as duplicates. Other genes consistently show evidence of homoeolog loss, suggesting repetitive aspects to polyploid genome evolution.

  17. Chemical healthcare waste management in small Brazilian municipalities.

    Science.gov (United States)

    Ferreira, João A; Bila, Danielle M; Ritter, Elisabeth; Braga, Ana Cs

    2012-12-01

    The disposal of healthcare waste (HCW) seems to have been solved in developed countries, while in most developing countries the problem persists because the disposal methods are expensive and larger than the budget of small- and medium-sized municipalities. The current study evaluates the encapsulation process for the disposal of medical chemical waste. The experiment was developed in the Piraí municipality (Rio de Janeiro State, Brazil) and the chemical wastes were produced in the local public hospital, as well as the 12 units of primary care health services. Chemical waste generated at health services units may include the liquid waste from cleaning materials and disinfectants, expired and unused pharmaceutical products, and cytotoxins. These are all considered hazardous waste products and they must be disposed of via an authorised system at approved sites (e.g. industrial landfills). The process of encapsulating chemical medical waste in concrete (cement, crushed stones and sand) followed by their disposal at sanitary landfills is a procedure that is not considered in Brazilian Legislation. Despite the oversight, this method of disposal was used in the municipality of Piraí, with the approval of the Rio de Janeiro State Agency for Environmental Control. The safety aspects of this method and the limits of its applicability, particularly in small municipalities, were evaluated in this study. The results indicate that, within certain parameters, this method may provide a viable solution for the disposal of HCW in small municipalities.

  18. Genetic variants of serum butyrylcholinesterase in Chilean Mapuche Indians.

    Science.gov (United States)

    Acuña, M; Eaton, L; Ramírez, N R; Cifuentes, L; Llop, E

    2003-05-01

    We estimated the frequencies of serum butyrylcholinesterase (BChE) alleles in three tribes of Mapuche Indians from southern Chile, using enzymatic methods, and we estimated the frequency of allele BCHE*K in one tribe using primer reduced restriction analysis (PCR-PIRA). The three tribes have different degrees of European admixture, which is reflected in the observed frequencies of the atypical allele BCHE*A: 1.11% in Huilliches, 0.89% in Cuncos, and 0% in Pehuenches. This result is evidence in favor of the hypothesis that BCHE*A is absent in native Amerindians. The frequencies of BCHE*F were higher than in most reported studies (3.89%, 5.78%, and 4.41%, respectively). These results are probably due to an overestimation of the frequency of allele BCHE*F, since none of the 20 BCHE UF individuals (by the enzymatic test) individuals analyzed showed either of the two DNA base substitutions associated with this allele. Although enzymatic methods rarely detect the presence of allele BCHE*K, PCR-PIRA found the allele in an appreciable frequency (5.76%), although lower than that found in other ethnic groups. Since observed frequencies of unusual alleles correspond to estimated percentages of European admixture, it is likely that none of these unusual alleles were present in Mapuche Indians before the arrival of Europeans. Copyright 2003 Wiley-Liss, Inc.

  19. Uso da taxocenose de peixes como indicadora de degradação ambiental no rio Paraíba do Sul, Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Francisco Gerson Araújo

    1998-01-01

    Full Text Available An adaptation of index of biotic integrity (IBI was performed for the first time in Brazil at the Rio Paraíba do Sul, between Barra Mansa and Barra do Piraí, a very important extent of this river because of the high pollution levels, and for being the main abstraction source of Rio de Janeiro city water supply. A monthly standardised sampling programme, from March-1995 to February-1996, was carried out at four sites. Seines, casting nets and net trays were used for catching fishes. IBI was obtained by integrating 12 ecological and environmental attributes of fish taxocene, populations, and individual organisms to assess biological integrity based in Species Composition, Trophic Structure and Fish Condition. Overall, water quality was classified between poor and fair and an improvement was detected between the high polluted Barra Mansa site and the less polluted Barra do Piraí site, showing the capacity of restoration of the river over this 50 km. Seasonally, the best conditions were reached from March to August, and the worse, from September to February, when most aloctone materials are brought to the river.

  20. Insulin receptor A and Sirtuin 1 synergistically improve learning and spatial memory following chronic salidroside treatment during hypoxia.

    Science.gov (United States)

    Barhwal, Kalpana; Das, Saroj K; Kumar, Ashish; Hota, Sunil K; Srivastava, Ravi B

    2015-10-01

    Hypoxia has been reported to cause hippocampal neurodegeneration resulting in learning and memory deficits. In the present study, we investigated the potential of salidroside, a glucoside derivative of tyrosol, in ameliorating hypoxia-induced neurodegeneration and memory impairment. Morris water maze test showed improvement in learning and spatial memory of salidroside-treated hypoxic rats correlating with increased dendritic intersections and arborization. Salidroside administration increased phosphorylation of insulin receptor subunit A (IRA) at Y972, Y1162/63, and Y1146 sites and subsequent activation of AMP-activated protein kinase (AMPK) α subunit isoforms pAMPKα1 and pAMPKα2 resulting in mitochondrial biogenesis. Contrarily, silencing of IRA in salidroside-supplemented hypoxic hippocampal cells could not improve cell viability or alter pAMPKα1 and pAMPKα2 expression. Rats administered with salidroside showed elevated expression of phosphorylated cAMP response element-binding protein in the hippocampus. Salidroside administration also resulted in increased sirtuin 1 (SIRT1) activity through a cytochrome P4502E1 (CYP2E1)-regulated mechanism that was independent of pIRA. Taken together, these findings suggest a synergistic role of pIRA and SIRT1 in salidroside-mediated neuroprotection, mitochondrial biogenesis, and cognitive improvement during hypoxia. We propose a novel mechanism for salidroside-mediated neuroprotection in hypoxia. © 2015 International Society for Neurochemistry.

  1. Produção e indicadores fisiológicos de alface sob hidroponia com água salina Production and physiologic indicators of lettuce grown in hydroponics with saline water

    Directory of Open Access Journals (Sweden)

    Dalva Paulus

    2010-03-01

    Full Text Available O uso de água salina na produção de hortaliças constitui no momento atividade essencial, tendo em vista o aumento da demanda de água doce, tanto pela atividade agrícola quanto pelo abastecimento urbano e industrial. O objetivo do trabalho foi avaliar a produção e os indicadores fisiológicos de alface cultivada em hidroponia com a utilização de água salina. O experimento foi conduzido em ambiente protegido, em Piracicaba-SP. O delineamento experimental foi de blocos ao acaso, sendo estudados os efeitos de cinco níveis de salinidade da água de irrigação, utilizando-se NaCl [Condutividade elétrica (Cea: 0,42, 1,53, 3,52, 5,55 e 7,43 dS m-1] em duas cultivares de alface (Verônica e Pira Roxa, em esquema fatorial. Foram determinadas massa fresca e seca de folhas, caule, raízes e da parte aérea; teor de nitrato, prolina e clorofila. O aumento da salinidade da água reduziu linearmente as massas fresca e seca das folhas, caule, raízes e da parte aérea. As massas fresca e seca foram 36% e 57% superiores na cultivar Verônica, respectivamente. A cultivar Pira Roxa apresentou maior teor de nitrato 25% (3008 mg L-1, clorofila total 50% (1,46 mg g-1massa fresca e prolina, 71,43% (0,21 µM g-1massa fresca-1 em relação à Verônica, o que pode ser um mecanismo de adaptação daquela cultivar ao estresse salino. Com relação à produtividade comercial, obteve-se uma perda de 69 e 64% para as cultivares Pira Roxa e Verônica, quando se utilizou água mais salina (7,43 dS m-1. Em relação à produção de massa seca, a perda pelo uso dessa água foi de 53% e 44%, respectivamente. Os resultados obtidos em sistema de cultivo hidropônico podem indicar a possibilidade do uso da água salina como alternativa para produção de hortaliças para produtores que têm disponibilidade de água salina e restrita disponibilidade de água doce, embora com redução na produtividade.The use of saline water in the production of vegetables constitutes

  2. ANÁLISE DOS PARÂMETROS DE FUNCIONAMENTO E SIMULAÇÃO COMPUTACIONAL EM UM PROTÓTIPO DE TURBINA OPERANDO A AR COMPRIMIDO

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    Wesley Saldanha Nogueira Oliveira

    2016-12-01

    Full Text Available RESUMO. Este trabalho apresenta os resultados iniciais da comparação de alguns parâmetros de funcionamento do protótipo de uma turbina de ação simples, operando com ar comprimido como fluido de trabalho, realizados na unidade do SENAI de Barra do Piraí/RJ. O protótipo foi desenvolvido integralmente no Centro Universitário Geraldo Di Biase - UGB - Campus de Barra do Piraí/RJ, que inicialmente teve como o fluido de trabalho o vapor d’água, que nos testes inicias se mostrou ineficiente. A simulação computacional do sistema foi feita através do software SolidWorks na Universidade Federal Fluminense - UFF - Campus de Volta Redonda/RJ, o que permitiu determinar a faixa de trabalho que apresentou maior eficiência nos resultados experimentais do sistema por meio da relação entre pressão de operação, velocidade angular transmitida pelo fluido ao eixo da turbina e a potência da mesma. Os valores nas simulações em velocidade tangencial na região rotacional apresentaram-se convergentes aos valores experimentais medidos no protótipo.

  3. Presence of two alternative kdr-like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na+ channel of Anopheles culicifacies from Orissa, India

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    Bhatt Rajendra M

    2010-05-01

    Full Text Available Abstract Background Knockdown resistance in insects resulting from mutation(s in the voltage gated Na+ channel (VGSC is one of the mechanisms of resistance against DDT and pyrethroids. Recently a point mutation leading to Leu-to-Phe substitution in the VGSC at residue 1014, a most common kdr mutation in insects, was reported in Anopheles culicifacies-a major malaria vector in the Indian subcontinent. This study reports the presence of two additional amino acid substitutions in the VGSC of an An. culicifacies population from Malkangiri district of Orissa, India. Methods Anopheles culicifacies sensu lato (s.l. samples, collected from a population of Malkangiri district of Orissa (India, were sequenced for part of the second transmembrane segment of VGSC and analyzed for the presence of non-synonymous mutations. A new primer introduced restriction analysis-PCR (PIRA-PCR was developed for the detection of the new mutation L1014S. The An. culicifacies population was genotyped for the presence of L1014F substitution by an amplification refractory mutation system (ARMS and for L1014S substitutions by using a new PIRA-PCR developed in this study. The results were validated through DNA sequencing. Results DNA sequencing of An. culicifacies individuals collected from district Malkangiri revealed the presence of three amino acid substitutions in the IIS6 transmembrane segments of VGSC, each one resulting from a single point mutation. Two alternative point mutations, 3042A>T transversion or 3041T>C transition, were found at residue L1014 leading to Leu (TTA-to-Phe (TTT or -Ser (TCA changes, respectively. A third and novel substitution, Val (GTG-to-Leu (TTG or CTG, was identified at residue V1010 resulting from either of the two transversions–3028G>T or 3028G>C. The L1014S substitution co-existed with V1010L in all the samples analyzed irrespective of the type of point mutation associated with the latter. The PIRA-PCR strategy developed for the

  4. Similar patterns of rDNA evolution in synthetic and recently formed natural populations of Tragopogon (Asteraceae allotetraploids

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    Soltis Pamela S

    2010-09-01

    Full Text Available Abstract Background Tragopogon mirus and T. miscellus are allotetraploids (2n = 24 that formed repeatedly during the past 80 years in eastern Washington and adjacent Idaho (USA following the introduction of the diploids T. dubius, T. porrifolius, and T. pratensis (2n = 12 from Europe. In most natural populations of T. mirus and T. miscellus, there are far fewer 35S rRNA genes (rDNA of T. dubius than there are of the other diploid parent (T. porrifolius or T. pratensis. We studied the inheritance of parental rDNA loci in allotetraploids resynthesized from diploid accessions. We investigate the dynamics and directionality of these rDNA losses, as well as the contribution of gene copy number variation in the parental diploids to rDNA variation in the derived tetraploids. Results Using Southern blot hybridization and fluorescent in situ hybridization (FISH, we analyzed copy numbers and distribution of these highly reiterated genes in seven lines of synthetic T. mirus (110 individuals and four lines of synthetic T. miscellus (71 individuals. Variation among diploid parents accounted for most of the observed gene imbalances detected in F1 hybrids but cannot explain frequent deviations from repeat additivity seen in the allotetraploid lines. Polyploid lineages involving the same diploid parents differed in rDNA genotype, indicating that conditions immediately following genome doubling are crucial for rDNA changes. About 19% of the resynthesized allotetraploid individuals had equal rDNA contributions from the diploid parents, 74% were skewed towards either T. porrifolius or T. pratensis-type units, and only 7% had more rDNA copies of T. dubius-origin compared to the other two parents. Similar genotype frequencies were observed among natural populations. Despite directional reduction of units, the additivity of 35S rDNA locus number is maintained in 82% of the synthetic lines and in all natural allotetraploids. Conclusions Uniparental reductions of

  5. Periodo de interferencia de arvenses en el cultivo de berenjena (Solanum melongena L.

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    Aramendiz-Tatis Hermes

    2010-04-01

    Full Text Available

    Dada la importancia de la berenjena en la horticultura del Caribe colombiano, el presente trabajo buscó establecer las bases de un manejo sustentable e integrado de malezas en este cultivo, basado en el período de máxima interferencia de aquellas sobre este. El estudio se realizó en la Universidad de Córdoba, ubicada en Montería (8°39’ N; 75°58’ W. El diseño experimental consistió en bloques completamente aleatorizados, con cuatro repeticiones y siete tratamientos relacionados con periodos crecientes de control de arvenses, seguidos por ausencia de control. Los resultados muestran que las arvenses con mayor presencia fueron paja mona (Leptochloa filiformis [Lam.] Beauv., caminadora (Rottboellia cochinchinensis [Lour.] W.D. Clayton, liendre de puerco (Echinochloa colona [L.] Link., coquito (Cyperus rotundus L. y bledo (Amaranthus retroflexus L.. Las arvenses afectaron la altura de la planta, el diámetro del tallo, y el rendimiento y calidad del fruto. Este último parámetro no se ve afectado cuando el cultivar lila se mantiene limpio durante los primeros 40 días. Al considerar el tipo de productor de esta hortaliza en el valle del Sinú, se sugiere utilizar: i para áreas pequeñas, métodos físicos, como el arranque manual o corte con machete u otra herramienta, y prácticas culturales; ii para áreas grandes, la combinación de herbicidas de presiembra con métodos físicos y culturales.

  6. Adaptación y rendimiento de plantas autóctonas de Guatemala en un sistema acuapónico

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    Carlos Valdez-Sandoval

    2017-03-01

    Full Text Available Se determinó la adaptación y rendimiento de ocho variedades de plantas autóctonas de Guatemala en un sistema acuapónico. Se utilizaron siete módulos acuapónicos, con capacidad de 80 plantas y 25 organismos de tilapia nilótica (Oreochromis niloticus. Se incluyeron en el estudio, plantas de apazote (Dysphania ambrosioides, makuy (Solanum nigrescens, amarantos (Amaranthus sp., bledo (A. cruentus, chipilín (Crotalaria longirostrata, chipilín montés (C. vitellina ayote (Cucurbita argyrosperma y güicoy (C. pepo. El periodo de evaluación fue de 60 días. Se registró la supervivencia (S, tamaño (T, peso (P y biomasa o rendimiento (B de las plantas y los peces al inicio y al final del periodo experimental. Se observó adaptación de siete de las ocho especies de plantas autóctonas, a los módulos acuapónicos experimentales. La variedad de planta con mayor crecimiento fue el ayote, seguida del makuy y el güicoy. Se observaron diferencias en la altura (p=0.00001 y el rendimiento (p=0.00001 de las variedades de las plantas. La biomasa de tilapia por tanque fue _ = 730 ± 75.2 g. En tal sentido, es posible cultivar plantas autóctonas de Guatemala en un sistema acuapónico.

  7. Lymphoplasmacytic myenteric, subepicardial, and pulmonary ganglioneuritis in four nonpsittacine birds.

    Science.gov (United States)

    Perpiñán, David; Fernández-Bellon, Hugo; López, Carlos; Ramis, Antoni

    2007-09-01

    A disease condition with clinical and pathologic findings compatible with psittacine proventricular dilatation disease was diagnosed in a canary (Serinus canaria), a greenfinch (Carduelis chloris), a long-wattled umbrellabird (Cephalopterus penduliger), and a bearded barbet (Lybius dubius). The canary and the greenfinch were kept as pets by different owners, whereas the bearded barbet and the long-wattled umbrellabird were kept in separate mixed species enclosures at the Barcelona Zoo. Clinical signs were variable in all 4 birds and included polyphagia, weight loss, weakness, and ataxia. Postmortem examination findings were also variable and included emaciation, hepatic and renal atrophy or enlargement, gallbladder dilatation, and intestinal and ventricular dilatation. Histopathologic lesions in all birds consisted of multifocal lymphoplasmacytic infiltration of myenteric and cardiac nerves and ganglia. These lesions are characteristic of proventricular dilatation disease of psittacine birds.

  8. Two New and Four Unrecorded Species of Chironomidae (Diptera in Korea

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    Han-Il Ree

    2012-01-01

    Full Text Available Chironomid adults attracted to the light were collected at Dangsan-ri, Muju-eup, Muju-gun, Jeollabuk-do in 2008-2009. Two new species, Lymnophyes parakitanaides sp. nov. and Parakiefferiella mujuensis sp. nov., and four unrecorded species, Cardiolcladius capusinus, Thienemanniella vittata, Conchapelopia pallidula, and Nilotanypus dubius were found and are described with illustrations. The genera Thienemanniella and Nilotanypus have not been previously reported in Korea. The genus Thienemanniella which belongs to Orthocladiinae is characterized by the radial sector retracted and apically fused with the costa, and the genus Nilotanypus which belongs to Tanypodinae is characterized by the absence of R2+3, pubescent eyes and lack of the gonocoxal lobes.

  9. Tomato spotted wilt virus (TS WV, weeds and thrip vectors in the tomato (Solanum lycopersicum L. in the Andean region of Cundinamarca (Colombia

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    Everth E Ebratt R

    2013-04-01

    Full Text Available The presence and distribution of the TSWV, weeds and thrip vectors in major tomato producing areas in the Andean department of Cundinamarca (Oriente, Sumapaz and Ubate provinces were assessed with the DAS ELISA technique, evaluating the presence of the TSWV in tomato tissue, associated thrips and weeds. High incidences were observed in different provinces of the Andean department of Cundinamarca. The average viral incidence reached 23.3% in Sumapaz, 19.4% in Oriente and 4% in Ubate. The symptoms observed were: brown spots and concentric rings in the leaf area, stems and fruits; browning and spotting in the flower; and wilting in the leaves, stems and flowers. The thrip species with the highest presence were Frankliniella occidentalis, followed by Thrips palmi and Thrips tabaci. We determined the important role of weeds as inoculum sources and vector reservoirs for the species Emilia sonchifolia and Amaranthus dubius

  10. Direct and indirect speech fusion in Charisius’ Ars grammatica

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    Marcos Antonio Pérez Alonso

    2012-06-01

    Full Text Available When transmitting the teaching of his predecessors, the grammarian Charisius usually makes use of either subordinating clauses or direct speech, but he hardly ever employs the so-called indirect speech, typically found in strictly literary works rather than in technical ones. However, we have encountered some passages in which portions in direct speech precede indirect speech clauses, a remarkable phenomenon which could be thought of as textual corruptions needing emendation. Nevertheless, from the fact that this phenomenon is mainly found in parts of Charisius’ work taken from Julius Romanus’ De analogía (where the latter quotes Pliny the Elder’s Dubius sermo, we conclude that the text is correct and that we are dealing with a type of citation characteristic of Julius Romanus, the description of which is not found in the literature on Latin Syntax.

  11. Meiotic Studies in Some Species of Tribe Cichorieae (Asteraceae from Western Himalayas

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    Raghbir Chand Gupta

    2014-01-01

    Full Text Available The present paper deals with meiotic studies in 15 species belonging to 6 genera of the tribe Cichorieae from various localities of Western Himalayas. The chromosome number has been reported for the first time in Hieracium crocatum (2n=10 and Lactuca lessertiana (2n=2x=16. Further, intraspecific variability has been reported for the first time in H. umbellatum (2n=2x=10 and 2n=6x=54, Tragopogon dubius (2n=2x=14 and 2n=4x=28, and T. gracilis (2n=2x=14. The chromosome report of 2n=2x=10 in Youngia tenuifolia is made for the first time in India. Maximum numbers of the populations show laggards, chromosome stickiness, and cytomixis from early prophase to telophase-II, leading to the formation of aneuploid cells or meiocytes with double chromosome number. Such meiotic abnormalities produce unreduced pollen grains and the reduced pollen viability.

  12. Meiotic studies in some species of tribe Cichorieae (Asteraceae) from Western Himalayas.

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    Gupta, Raghbir Chand; Goyal, Henna; Singh, Vijay; Goel, Rajesh Kumar

    2014-01-01

    The present paper deals with meiotic studies in 15 species belonging to 6 genera of the tribe Cichorieae from various localities of Western Himalayas. The chromosome number has been reported for the first time in Hieracium crocatum (2n = 10) and Lactuca lessertiana (2n = 2x = 16). Further, intraspecific variability has been reported for the first time in H. umbellatum (2n = 2x = 10 and 2n = 6x = 54), Tragopogon dubius (2n = 2x = 14 and 2n = 4x = 28), and T. gracilis (2n = 2x = 14). The chromosome report of 2n = 2x = 10 in Youngia tenuifolia is made for the first time in India. Maximum numbers of the populations show laggards, chromosome stickiness, and cytomixis from early prophase to telophase-II, leading to the formation of aneuploid cells or meiocytes with double chromosome number. Such meiotic abnormalities produce unreduced pollen grains and the reduced pollen viability.

  13. [The issue of medico-legal assessment of noise induced hearing loss: comparison of methods].

    Science.gov (United States)

    Bosio, D; Coggiola, M; Baracco, A; Andreis, P; Perrelli, F

    2011-01-01

    Audiogram classification is crucial for hearing protection of workers occupationally exposed to noise. The methods that have been proposed are based on two principles: the morphological evaluation of the audiometric curve (eg. Merluzzi-Pira-Bosio--MPB) or the average hearing loss on different frequencies (eg. Albera-Beatrice--AB). The purpose of this study was to classify audiograms compatible with chronic acoustic trauma performed at the Occupational Medicine Outpatient Clinic of CTO Hospital in Turin from 2004 to 2011 with the methods outlined in Guidelines published by SIMLII. A substantial agreement among the methods was observed. While MPB is the most appropriate method for secondary prevention, the AB would seem more appropriate for the verification of a permanent weakening that has to be reported to the competent legal authorities.

  14. Monogenoidea en Serrasalmus rhombeus (Linnaeus, 1766 de la Cuenca Amazónica Boliviana

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    Leslie Córdova

    2013-04-01

    Full Text Available El presente trabajo analiza la diversidad de parásitos monogenoideos como un carácter indepen- diente, esperando que por su alta especifi cidad, éstos parásitos puedan caracterizar las poblaciones de sus hospederos, la piraña blanca, Serrasalmus rhombeus . El estudio se llevó acabo en cuatro sub-cuencas de la Cuenca Amazónica Boliviana (Iténez, Beni, Madre de Dios e Ichilo; se obtuvie- ron 1135 parásitos, los cuales fueron agrupados en seis géneros y once especies: Anacanthorus jegui, A. amazonicus , A. rondonensis, A. gravihamulatus, A. sciponophallus, Anacanthorus sp.1 , Anacanthorus sp.2 , Anacanthorus sp.3 ; Notothecium deleastum, N. deleastoideum, Notozothecium teinodendrum; Mymarothecium galeolum; Anphithecium diclonophallum y Enallothecium aegidatum. La elevada diversidad de parásitos encontrada puede deberse a que existe o existió un complejo de especies o poblaciones diferenciadas de Serrasalmus rhombeus.

  15. Effect of Growth on Fatty Acid Composition of Total Intramuscular Lipid and Phospholipids in Ira Rabbits.

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    Xue, Shan; He, Zhifei; Lu, Jingzhi; Tao, Xiaoqi; Zheng, Li; Xie, Yuejie; Xiao, Xia; Peng, Rong; Li, Hongjun

    2015-01-01

    The changes in fatty acid composition of total intramuscular lipid and phospholipids were investigated in the longissimus dorsi, left-hind leg muscle, and abdominal muscle of male Ira rabbits. Changes were monitored at 35, 45, 60, 75, and 90 d. Analysis using gas chromatography identified 21 types of fatty acids. Results showed that the intramuscular lipid increased and the intramuscular phospholipids (total intramuscular lipid %) decreased in all muscles with increasing age (pIra rabbits at different ages and muscles. Palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), and arachidonic acid (C20:4) were the major fatty acids, which account to the dynamic changes of the n-6/n-3 value in Ira rabbit meat.

  16. La configuración de un modelo : los catafalcos madrileños durante el reinado de Felipe V

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    Victoria Soto Caba

    1989-01-01

    Full Text Available En el último tercio del siglo xvii la tendencia decorativista y la variedad tipológica fueron las características más acusadas de los aparatos provisionales de las exequias reales. Las tradiciones locales y los talleres barrocos mantuvieron ambos rasgos durante gran parte de la centuria siguiente. El tapizado ornamental recubrió túmulos en forma de torre, pira, templetes, baldaquinos... Estas estructuras heredadas apenas dejaron sitio para nuevas variantes arquitectónicas, por lo que se puede decir que el siglo xviii, al menos la primera mitad, contribuyó muy poco en el enriquecimiento del repertorio tipológico de los catafalcos reales.

  17. A reassessment of the taxonomic status of Paraglyptodon Castellanos, 1932 (Mammalia, Cingulata, Glyptodontia)

    Science.gov (United States)

    Cruz, Laura E.; Fernicola, Juan C.; Taglioretti, Matias; Toledo, Nestor

    2016-03-01

    Castellanos described and published about new genera of glyptodonts, according to a phylogenetic scheme mainly based on the evolution of the external surface of the dorsal carapace. Among these new genera, Castellanos proposed Paraglyptodon as the predecessor of Glyptodon, and included within Paraglyptodon all known species of Glyptodontinae recovered from "horizontes pre-Ensenadenses", and within Glyptodon all known species from "Horizontes pampeanos", restricting the latter to the Quaternary. All the species that belong to Paraglyptodon, that is Paraglyptodon chapalmalensis, Paraglyptodon uquiensis, Paraglyptodon dubius, and Paraglyptodon paranensis were established based on one, two or few osteoderms, mostly from the dorsal carapace. Regarding P. paranensis and P. dubius, Oliva and collaborators consider the first as a nomen vanum, representing an indeterminate Glyptodontinae, and the second as a synonym of P. chapalmalensis. Upon re-examination of the holotypes of P. chapalmalensis and P. uquiensis together with their comparison with other well-known specimens of glyptodonts, mainly with Glyptodon (of both juvenile and adult stages), we found the same ornamentation in different sections of the dorsal carapaces, particularly in P. chapalmalensis and in juvenile stages of Glyptodon spp. We could not identify features that would allow us to make a distinction between the holotype of P. uquiensis and Glyptodon spp. Therefore, we consider that a new taxon guide for naming the Upper Chapadmalalan biozone is necessary. The biostratigraphic range of Glyptodon could possibly be extended to the late Pliocene. However, new records and studies are needed to verify the existence of this taxon in the Chapadmalalan Stage/Age in its type locality.

  18. Intra-Annual Variation in Responses by Flying Southern Pine Beetles (Coleoptera: Curculionidae: Scolytinae) to Pheromone Component endo-Brevicomin.

    Science.gov (United States)

    Sullivan, Brian T; Brownie, Cavell; Barrett, JoAnne P

    2016-08-01

    The southern pine beetle Dendroctonus frontalis Zimmermann (Coleoptera: Curculionidae: Scolytinae) is attracted to an aggregation pheromone that includes the multifunctional pheromone component endo-brevicomin. The effect of endo-brevicomin on attractive lures varies from strong enhancement to reduction of beetle attraction depending upon release rate, lure component spacing, and proximity of beetle infestations. Anecdotal observations have further suggested that the effects of endo-brevicomin vary during the year. We investigated this possibility under nonoutbreak conditions in southwestern Mississippi where for two-and-a-half years we monitored traps baited with frontalin and the host odor alpha-pinene either (a) alone, or with an endo-brevicomin release device either (b) located directly on the trap, or (c) displaced 6 m away. The endo-brevicomin devices in our tests increased D. frontalis catches during all times of year, and 6 m displacement of the endo-brevicomin release device from the trap did not significantly alter responses except during the spring flight peak when displacement increased catches. Our data suggest that flying D. frontalis have a stronger tendency to avoid the immediate proximity of a release point of endo-brevicomin during their springtime dispersal flight when catches are greatest. Catches of Thanasimus dubius (F.) (Coleoptera: Cleridae), a major predator of D. frontalis, were not altered by endo-brevicomin, and ratios of D. frontalis to T. dubius changed over the course of the year. We discuss the possible effects of intra-annual variation in D. frontalis response to endo-brevicomin both on beetle attack behavior and use of endo-brevicomin as a lure adjuvant in D. frontalis population monitoring.

  19. Distribución e identificación de especies hospedantes de Heterodera glycines Ichinohe raza 3 en el Valle del Cauca

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    Varón de Agudelo Francia

    1988-06-01

    Full Text Available Se dividió la parte plana del Valle del Cauca en tres zonas (norte, centro y sur, habiéndose visitado 33 fincas. En la zona norte las malezas con mayor porcentaje de frecuencia y distribución en los cultivos de soya fueron Digitaria horizontalis, Echinochloa colonum y Leptochloa filiformis; en la zona centro Ipomoea hirta, Amaranthus dubius y Echinochloa colonum y en la zona sur predominaron Ipomoea hirta, Portulaca oleracea Cyperus rotundus. Los análisis de muestras de suelo y raíces indicaron que H. glycines se encuentra distribuido en todo el Valle del Cauca, presentando la zona sur (Candelaria, Palmira y Puerto Tejada las mayores poblaciones. Entre las especies evaluadas (malezas, cultivos, leguminosas forrajeras y silvestres, solamente Glycine max y Phaseolus vulgaris se consideraron como susceptibles a H. glycines raza 3. y P. angularis y P. multiflora permitieron muy poca infección y multiplicación del nemátodo.A nematode recognition of Heterodera glycines was focused on crops of soybean. Valle del Cauca was divided in three zones (northen, central and southern and 33 farms were visited. The results of the analysis on samples of soils and roots showe that Heterodera glycines is scattered throughout Valle del Cauca, being the southern zone (Palmira, Candelaria and Puerto Tejada the one having the highest standards in nematode population. Weeds showing a greater frequency percentage were : Digitaria horizontalis, Echinochloa colonum and Leptochloa filiformis, in the northen zone; Ipomoea hirta, Amaranthus dubius and Echinochloa colonum, in the central zone, and Ipomoea hirta, Portulaca oleracea and Cyperus rotundus, in the southern zone , From among the whole species evaluated (weeds, crops, leguminous a n d fodder plants, Glycine max and Phaseolus vulgaris were considered to be susceptible to H. Glycines race 3. Phaseolus angularis y P. multiflora let low population levels.

  20. Characterization and PCR Detection Of Binary, Pir-Like Toxins from Vibrio parahaemolyticus Isolates that Cause Acute Hepatopancreatic Necrosis Disease (AHPND) in Shrimp

    Science.gov (United States)

    Sirikharin, Ratchanok; Taengchaiyaphum, Suparat; Sanguanrut, Piyachat; Chi, Thanh Duong; Mavichak, Rapeepat; Proespraiwong, Porranee; Nuangsaeng, Bunlung; Thitamadee, Siripong; Flegel, Timothy W.; Sritunyalucksana, Kallaya

    2015-01-01

    Unique isolates of Vibrio parahaemolyticus (VPAHPND) have previously been identified as the causative agent of acute hepatopancreatic necrosis disease (AHPND) in shrimp. AHPND is characterized by massive sloughing of tubule epithelial cells of the hepatopancreas (HP), proposed to be induced by soluble toxins released from VPAHPND that colonize the shrimp stomach. Since these toxins (produced in broth culture) have been reported to cause AHPND pathology in reverse gavage bioassays with shrimp, we used ammonium sulfate precipitation to prepare protein fractions from broth cultures of VPAHPND isolates for screening by reverse gavage assays. The dialyzed 60% ammonium sulfate fraction caused high mortality within 24–48 hours post-administration, and histological analysis of the moribund shrimp showed typical massive sloughing of hepatopancreatic tubule epithelial cells characteristic of AHPND. Analysis of the active fraction by SDS-PAGE revealed two major bands at marker levels of approximately 16 kDa (ToxA) and 50 kDa (ToxB). Mass spectrometry analysis followed by MASCOT analysis revealed that both proteins had similarity to hypothetical proteins of V. parahaemolyticus M0605 (contig034 GenBank accession no. JALL01000066.1) and similarity to known binary insecticidal toxins called 'Photorhabdus insect related' proteins A and B (Pir-A and Pir-B), respectively, produced by the symbiotic, nematode bacterium Photorhabdus luminescens. In in vivo tests, it was shown that recombinant ToxA and ToxB were both required in a dose dependent manner to cause AHPND pathology, indicating further similarity to Pir-A and -B. A single-step PCR method was designed for detection of the ToxA gene and was validated using 104 bacterial isolates consisting of 51 VPAHPND isolates, 34 non-AHPND VP isolates and 19 other isolates of bacteria commonly found in shrimp ponds (including other species of Vibrio and Photobacterium). The results showed 100% specificity and sensitivity for detection of

  1. Characterization and PCR Detection Of Binary, Pir-Like Toxins from Vibrio parahaemolyticus Isolates that Cause Acute Hepatopancreatic Necrosis Disease (AHPND in Shrimp.

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    Ratchanok Sirikharin

    Full Text Available Unique isolates of Vibrio parahaemolyticus (VPAHPND have previously been identified as the causative agent of acute hepatopancreatic necrosis disease (AHPND in shrimp. AHPND is characterized by massive sloughing of tubule epithelial cells of the hepatopancreas (HP, proposed to be induced by soluble toxins released from VPAHPND that colonize the shrimp stomach. Since these toxins (produced in broth culture have been reported to cause AHPND pathology in reverse gavage bioassays with shrimp, we used ammonium sulfate precipitation to prepare protein fractions from broth cultures of VPAHPND isolates for screening by reverse gavage assays. The dialyzed 60% ammonium sulfate fraction caused high mortality within 24-48 hours post-administration, and histological analysis of the moribund shrimp showed typical massive sloughing of hepatopancreatic tubule epithelial cells characteristic of AHPND. Analysis of the active fraction by SDS-PAGE revealed two major bands at marker levels of approximately 16 kDa (ToxA and 50 kDa (ToxB. Mass spectrometry analysis followed by MASCOT analysis revealed that both proteins had similarity to hypothetical proteins of V. parahaemolyticus M0605 (contig034 GenBank accession no. JALL01000066.1 and similarity to known binary insecticidal toxins called 'Photorhabdus insect related' proteins A and B (Pir-A and Pir-B, respectively, produced by the symbiotic, nematode bacterium Photorhabdus luminescens. In in vivo tests, it was shown that recombinant ToxA and ToxB were both required in a dose dependent manner to cause AHPND pathology, indicating further similarity to Pir-A and -B. A single-step PCR method was designed for detection of the ToxA gene and was validated using 104 bacterial isolates consisting of 51 VPAHPND isolates, 34 non-AHPND VP isolates and 19 other isolates of bacteria commonly found in shrimp ponds (including other species of Vibrio and Photobacterium. The results showed 100% specificity and sensitivity for

  2. 肾动脉各级血流多普勒参数反映肝硬化病程

    Institute of Scientific and Technical Information of China (English)

    楼丽萍; 徐晓飞; 王洁; 方蕾

    2013-01-01

    目的 应用彩色多普勒超声分析肾动脉血流动力学信息,了解肝硬化病情的发展.方法 选择肝硬化患者60例为肝硬化组,根据病程进展分为Ⅰ、Ⅱ、Ⅲ组.同期20位健康体检者为对照组,运用彩色多普勒超声进行肾动脉检查,并记录血流参数.结果 随着肝硬化病情的逐渐发展,肾主动脉(main renal artery,MRA)、段动脉(segmental renal artery,SRA)、叶间动脉(interlobar renal artery,IRA)的阻力指数(resistance index,RI)逐渐增加,差异均有统计学意义(FMRA=41.34,PMRA=0.000;FSRA=26.41,PSRA=0.000;FIRA=71.95,PIRA=0.000);MRA、SRA、IRA的搏动指数(pulsatility index,PI)逐渐增加,差异均有统计学意义(FMRA=51.90,PMRA=0.000;FSRA=72.45,PSRA=0.000;FIRA=125.31,PIRA=0.000).血流量均显著降低,差异有统计学意义(F=18.66,P=0.000).结论 通过监测肝硬化进程中肾动脉血流参数,可以为临床监护病情、客观评估病情和预后提供有力证据.

  3. THE AGE AND ENVIRONMENT STUDY OF Gigantopithecus FAUNA OF PA'ERYAN, BIJIE, GUIZHOU, BASED ON THE CARNIVORE FOSSILS%贵州毕节扒耳岩巨猿动物群的年代与环境——来自食肉类化石的分析和研究

    Institute of Scientific and Technical Information of China (English)

    刘金毅; 赵凌霞; 陈津; 王新金; 蔡回阳; 张忠文

    2011-01-01

    Pa'eryan, a cave or fissure deposit at Bijie, Guizhou Province ( 27°22'12"N, 105°15'16"E, Fig. 1 ) , was identified as a Gigantopithecus site with of fossil mammals accumulation. Most carnivores, although small in number with only 19 pieces unearthed, are in good conditions; some of them can even be classified to species level. The carnivores unearthed from Pa'eryan include: hyaena ( Pachycrocuta licenti), black bear ( Ursus thibetanus primitinus) ,giant panda(Ailuropoda microta) ,jackal( Cuon dubius)and leopard(Panthera sp. ) ,namely 4 families, 5 genera and 5 species.The present fossil record indicates that Pachycrocuta licenti, Ailuropoda microta, Cuon dubius and Ursus thibetanus primitinus were endemic elements of the Early Pleistocene fauna in South China. The time period of these animals was short and only in the Early Pleistocene. Those of Ailuropoda microta and Cuon dubius were even shorter and possibly limited to the early Early Pleistocene. It can definitely be confirmed that Pa'eryan fauna is in Early Pleistocene according to its carnivore guild.All species mentioned above, except Panthera sp., are extinct species or subspecieces, which is a typical feature of mammalian assemblage of Early Pleistocene. The indexes for the extinct species and genera of Pa'eryan's carnivore guild are 80% and 20% respectively, which are similar to those of Juyuandong ( Liucheng) and Mehuidong ( Tiandong) , but somewhat lower than those from Longgupo ( Wushan) and higher than those from Longgudong( Jianshi) (Table 4). Moreove,the carnivore guild of Pa'eryan has 5 common or similar members with Juyuandong and Mehuidong, 4 with Longgupo ( Wushan ) , and only 3 with Longgudong ( Jianshi). The carnivore guild of Pa'eryan differs from that of Longgupo in hyaena fossils. The hyaenas from Longgupo are more primitive in morphology than those from Pa'eryan,and even identified as a different species,namely Pachycrocuta perrieri. The major differences between Pa'eryan and Longgudong

  4. Estilos de vida de pacientes hipertensos atendidos com la Estrategia de Salud de la Familia Estilos de vida de pacientes hipertensos atendidos com a Estratégia de Saúde Familiar Hypertensive patients’ attended with the family health strategy lifestyle

    Directory of Open Access Journals (Sweden)

    Elisabete Pimenta Araujo Paz

    2011-11-01

    Full Text Available Objetivo. Describir los estilos de vida de los pacientes hipertensos atendidos con la Estrategia de Salud de La Familia. Metodología. De octubre de 2009 a enero de 2010, se llevó a cabo un estudio transversal, en el que participaron 273 pacientes hipertensos seleccionados aleatoriamente del programa de salud familiar que atiende tres áreas de Piraí/RJ. Para la recolección de datos se utilizó el cuestionario "Estilo de Vida Fantástico". Resultados. De la muestra de estudio: el 61% eran mujeres, el 56% con 60 y más años, el 81% tenía bajo nivel de educación y 74% con bajos ingresos familiares. Los factores de riesgo más frecuentes fueron El sobrepeso u obesidad (72% y la diabetes mellitus (37%. La calificación del estilo de vida fue: 13% excelente, 55% muy bueno, 27% bueno y regular 4%. Conclusión. Aunque el estilo de invida se consideró satisfactorio, las condiciones socioeconômicas y el perfil de salud indican la persistencia de factores de riesgo de enfermedad cardiovascular.Objetivo. Descrever os estilos de vida dos pacientes hipertensos atendidos com a estratégia de Saúde Familiar. Metodologia. De outubro de 2009 a janeiro de 2010, levou-se a cabo um estudo transversal, no que participaram 273 pacientes hipertensos selecionados aleatoriamente do programa de Saúde Familiar que atende três áreas de Piraí/RJ (Brasil. Para a colheita de dados se utilizou o questionário "Estilo de Vida Fantástico". Resultados. Da mostra de estudo: o 61% eram mulheres, o 56% tinha 60 e mais anos, o 81% possuía sob nível de educação e 74% reportou ter baixos rendimentos familiares. Os fatores de risco mais frequentes foram o sobrepeso ou obesidade (72% e a diabete mellitus (37%. A qualificação do estilo de vida foi: 13% excelente, muito bom 55%, bom 27% e regular 4%. Conclusão. Ainda que o estilo de vida se considerou satisfatório, as condições e o perfil de saúde indicam a persistência de fatores de risco de doen

  5. Comparison of metering mechanisms of corn seed Comparação entre mecanismos dosadores de sementes de milho

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    Pedro H. Weirich Neto

    2012-10-01

    Full Text Available It was compared the performance of the metering mechanism of corn seeds (Zea mays in direct seeding in an area of ​​200 ha, a property in Piraí do Sul, State of Paraná - PR, in Brazil. It was seeded 4 maize hybrids, 50 ha of each, with seeds of different sieves. The experiment was conducted in a randomized block design (RBD, with 3 treatments and 9 repetitions for each corn hybrid. The treatments were the pneumatic seed of metering mechanisms, horizontal perforated disc with and without ramp®. The plots were 40 m² and were distributed at the early, middle and late sowing. The variables analyzed in each corn hybrid were initial population, faulty spacing, multiple spacing, acceptable spacing, and yield components. As there were no significant differences in the variables, it was concluded that the quality of seeding with different systems of distribution was similar in the different sieves of distributed corn seeds.Comparou-se o desempenho dos mecanismos dosadores de sementes de milho (Zea mays em semeadura direta, numa área de 200 ha, numa propriedade do município de Piraí do Sul - PR. Semearam-se quatro híbridos de milho, 50 ha cada, com sementes de peneiras distintas. O experimento foi conduzido no delineamento em blocos ao acaso (DBC, sendo três tratamentos e nove repetições para cada híbrido de milho. Os tratamentos consistiram nos mecanismos dosadores de sementes pneumáticos, disco perfurado horizontal com e sem rampa®. As parcelas tinham 40 m² e foram distribuídas no início, meio e final da semeadura. As variáveis analisadas em cada híbrido de milho foram: população inicial, espaçamentos falhos, espaçamentos múltiplos, espaçamentos aceitáveis e os componentes de rendimento. Como não houve diferenças significativas nas variáveis analisadas, concluiu-se que a qualidade de semeadura com diferentes sistemas de distribuição foi semelhante nas distintas peneiras de sementes de milho distribuídas.

  6. 京郊部分牛场荷斯坦母牛脊椎畸形综合征(CVM)遗传缺陷检测%Identiifcation of Complex Vertebral Malformation (CVM) Carriers in Holstein Cows from Suburban Farms in Beijing

    Institute of Scientific and Technical Information of China (English)

    唐韶青; 梁若冰; 云鹏; 杨宇泽; 肖炜; 孙东晓

    2014-01-01

    Complex vertebral malformation (CVM) is a monogenic autosomal recessive hereditary defect of Holstein dairy cattle. It is caused by a point mutation from G to T at the 4 extron in bovine solute carrier family 35, member 3 gene (SLC35A3). Homozygous recessive can cause early pregnancy abortion, stillbirth, or cow calf birth deformities, but carriers are normal. Polymerase chain reaction–primer introduced restriction analysis (PIRA-PCR) was used to detect CVM among 552 Holstein cows from Beijing. As a result, a total of 21 CVM carriers were identiifed. The frequency of carriers was 3.80% and the recessive allele frequency of CVM was 1.90%. Our pedigree studies of the carrier cattle in this experiment revealed that, out of 21 CVM carriers, 13 ones inherited mutation from those mothers and 15 ones were found to be the offspring of Carlin-M Ivanhoe Bell (USA1667366). Based on this, in our country, it is necessary to detect CVM among Holstein cows to prevent the CVM carriers mating and reduce economic losses.%牛脊椎畸形综合征(CVM)是由牛3号染色体上SLC35A3基因第4外显子上的G/T突变引起的隐性遗传疾病,其隐性纯合子可以造成母牛妊娠早期流产、死胎或出生犊牛畸形,而CVM携带者表型正常,无发病表现。本研究利用PIRA-PCR方法对北京地区552头母牛样本进行了检测分析。研究结果表明,所检测的样本中,荷斯坦母牛CVM携带者为21头,携带率为3.80%,有害基因频率为1.90%。通过系谱分析,其中13头携带者的致病基因来源于其母亲,15头CVM携带者为Carlin-MIvanhoeBell(USA1667366)的后代。因此,牛场应当开展母牛CVM筛查,防止CVM携带个体之间的交配,减少经济损失。

  7. Seleção de clones de batata-doce resistentes a insetos de solo Screening of sweet potato clones resistant to soil insects

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    José Ricardo Peixoto

    1999-03-01

    Full Text Available O objetivo deste trabalho foi selecionar clones comerciais de batata-doce para a região do Triângulo Mineiro. Dos clones avaliados, 60 foram obtidos por policruzamentos e cedidos pela Universidade Federal de Lavras (UFLA, três, entre produtores rurais dos municípios de Araguari, Uberlândia e Machado, todos do Estado de Minas Gerais, e outros nove foram usados como testemunhas (Brazlândia Branca, Brazlândia Rosada, Surpresa, Rio Doce, Morena Roxa, Coquinho, Arroba, Pira 1 e o Clone 042. O experimento foi instalado no espaçamento de 1,10 x 0,45 m, utilizando-se o delineamento blocos casualizados, com 72 tratamentos, quatro repetições e 16 plantas por parcela. Entre os clones avaliados 32,8% apresentaram alta ou moderada resistência a insetos de solo. O clone 95041 foi o mais produtivo, com 28.048,96 kg/ha. Os clones 95009, 95010, 95014, 95020, 95033, 95042 e 95057 foram altamente resistentes a insetos de solo.A research was carried out with the objective of selecting commercial clones for weather conditions of the Triângulo Mineiro, Minas Gerais State, Brazil. Sixty of the clones evaluated were obtained by polycross and given by the Universidade Federal de Lavras, three were from the farmers of the municipalities of Araguari, Uberlândia and Machado, located in Minas Gerais State, and nine clones were used as standards (Brazlândia Branca, Brazlândia Rosada, Surpresa, Rio Doce, Morena Roxa, Coquinho, Arroba, Pira 1 and Clone 042. The experiment was installed in a spacing of 1.10 x 0.45 m, using a randomized block design, with 72 treatments, four replicates and 16 plants per plot. Among the evaluated clones, 32.8% showed high or moderate resistance to soil insects. The clone with the highest yield was the clone number 95041, which produced 28,048.96 kg/ha. The others, 95009, 95010, 95014, 95020, 95033, 95042 and 95057 were highly resistant to soil insects.

  8. PCR-based methods for the detection of L1014 kdr mutation in Anopheles culicifacies sensu lato

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    Dash Aditya P

    2009-07-01

    Full Text Available Abstract Background Anopheles culicifacies s.l., a major malaria vector in India, has developed widespread resistance to DDT and is becoming resistant to pyrethroids–the only insecticide class recommended for the impregnation of bed nets. Knock-down resistance due to a point mutation in the voltage gated sodium channel at L1014 residue (kdr is a common mechanism of resistance to DDT and pyrethroids. The selection of this resistance may pose a serious threat to the success of the pyrethroid-impregnated bed net programme. This study reports the presence of kdr mutation (L1014F in a field population of An. culicifacies s.l. and three new PCR-based methods for kdr genotyping. Methods The IIS4-IIS5 linker to IIS6 segments of the para type voltage gated sodium channel gene of DDT and pyrethroid resistant An. culicifacies s.l. population from the Surat district of India was sequenced. This revealed the presence of an A-to-T substitution at position 1014 leading to a leucine-phenylalanine mutation (L1014F in a few individuals. Three molecular methods viz. Allele Specific PCR (AS-PCR, an Amplification Refractory Mutation System (ARMS and Primer Introduced Restriction Analysis-PCR (PIRA-PCR were developed and tested for kdr genotyping. The specificity of the three assays was validated following DNA sequencing of the samples genotyped. Results The genotyping of this An. culicifacies s.l. population by the three PCR based assays provided consistent result and were in agreement with DNA sequencing result. A low frequency of the kdr allele mostly in heterozygous condition was observed in the resistant population. Frequencies of the different genotypes were in Hardy-Weinberg equilibrium. Conclusion The Leu-Phe mutation, which generates the kdr phenotype in many insects, was detected in a pyrethroid and DDT resistant An. culicifacies s.l. population. Three PCR-based methods were developed for kdr genotyping. All the three assays were specific. The ARMS method

  9. Trabalho rural e fatores de risco associados ao regime de uso de agrotóxicos em Minas Gerais, Brasil Rural work and risk factors associated with pesticide use in Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Wagner Soares

    2003-08-01

    Full Text Available O objetivo deste artigo foi caracterizar o processo do trabalho rural em nove municípios de Minas Gerais, considerando indicadores sócio-demográficos, a estrutura agrária dos estabelecimentos rurais, práticas de trabalho relacionadas ao uso de agrotóxicos e, a intoxicação associada a seu uso. Os dados foram obtidos de uma pesquisa realizada pela Fundação Jorge Duprat Figueiredo, de Segurança e Medicina do Trabalho, que aplicou um questionário a 1.064 trabalhadores rurais, entre os anos de 1991 a 2000. Por meio de uma regressão logística, foram obtidos os fatores de risco associados à intoxicação por agrotóxicos dos grupos organofosforados e carbamatos. Cerca de 50% dos entrevistados se encontravam ao menos moderadamente intoxicados. Os fatores de risco encontrados foram: ter o último contato a menos de duas semanas com agrotóxicos; não usar proteção; ser orientado pelo vendedor; citar organofosforado ou carbamato como agrotóxico principal e trabalhar nos municípios de Teófoli Otoni, Guidoval ou Piraúba. Os resultados apontam para o alto grau de risco de agravos à saúde a que estão sujeitos trabalhadores rurais em contato com agrotóxicos.This paper aimed to characterize the rural work process in nine counties in Minas Gerais State, considering socio-demographic indicators, the land tenure structure of farm operations, and work practices related to pesticide use and poisoning Data were obtained through the Jorge Duprat Figueiredo Foundation for Workers' Safety and Occupational Medicine, which applied a questionnaire to 1,064 rural workers from 1991 to 2000. Through a logistic regression, risk factors associated with pesticide poisoning were obtained for the carbamate and organophosphate groups. Some 50% of interviewees displayed at least moderate pesticide toxicity. Risk factors for pesticide poisoning were: last contact with pesticides less than two weeks before the blood cholinesterase test; no use of personal

  10. Disponibilidade hídrica e germinação de sementes de cebola (Allium cepa L. Water availability and germination of onion seeds (Allium cepa L.

    Directory of Open Access Journals (Sweden)

    Z. Piana

    1994-12-01

    Full Text Available Objetivando verificar a influência do teor de água do substrato na germinação de sementes de cebola (Allium cepa L., conduziu-se um ensaio sob condições de ambiente de laboratório. Os efeitos de cinco níveis de água no substrato solo x areia (3:1, 20, 40, 60, 80 e 100% da capacidade de retenção foram avaliados através da porcentagem de emergência no 14° dia da semeadura e pesos da matéria verde e seca das plântulas normais. Para tanto, 200 sementes do cultivar Pira Ouro por tratamento, em quatro repetições de 50, foram semeadas a 1,5 cm de profundidade, mantendo-se constantes os níveis de água no substrato. Os resultados possibilitaram concluir que os níveis de umidade do solo indicados para germinação de sementes de cebola são de 40% e 60% da capacidade de retenção do substrato, por possibilitarem uma melhor emergência, peso da matéria verde e seca das plântulas.One essay was conducted under laboratory conditions, in order to verify the influence of substrate water content on the germination of onion (Allium cepa L. seeds. The effect of five water levels in the medium soil x sand (3:1 - 20, 40, 60, 80 and 100% retention capacity-were evaluated through the emergence percentage 14 days after seedling and the weight of fresh and dry matters of normal seedlings. For that purpose, 200 treated seeds of Pira Ouro cultivar were sown 1.5 cm deep in four replicátions of 50, with a constant water content in the medium. The best results showed that the soil moisture levels suggested for the germination of onion seeds were 40% to 60% of the substrate retention capacity, once they provide better emergence, weight of the fresh and dry matters of seedlings.

  11. Esputos de papel. La historieta ‘underground’ española

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    Dopico, Pablo

    2011-09-01

    Full Text Available To know the origin and development of contemporary Spanish comic, we get into the suburbs of visual culture to discover the history of Spanish underground comics. A comic with which, thanks to the relative freedom of expression offered, the counterculture found a versatile vehicle that could express themselves without problems, becoming a new form of social protest and claim it offers an invaluable testimony to the Spanish reality, superior to that afforded other contemporary arts. The text examines the main features, aesthetic aspects and new language of Spanish comix, discovering the main authors (Ceesepe, Gallardo, Mariscal, Max and Nazario, series (Makoki, The Garriris, Gustavo, Anarcoma,... and magazines (El Rrollo Enmascarado, Star, La Piraña Divina, Butifarra!, Los Tebeos del Rrollo, Rock Comix, El Víbora,…, and showing its connection with other arts, as painting, cinema and photography, not to mention its relationship to the world of drugs and rock music.

    Para conocer el origen y desarrollo de la historieta española contemporánea, debemos adentrarnos en los suburbios de la cultura visual. Así descubriremos la historia del cómic underground español. Un cómic donde, gracias a la relativa libertad de expresión que ofrecía, la contracultura encontró un vehículo versátil con el que podía expresarse sin trabas, convirtiéndose en una nueva forma de protesta y reivindicación social que ofreció un impagable testimonio de la realidad española, superior al que ofrecen otras artes coetáneas. El artículo analiza las principales características, aspectos estéticos y novedades lingüísticas del comix español, descubriendo sus principales autores (Ceesepe, Gallardo, Mariscal, Max y Nazario, series (Makoki, Los Garriris, Gustavo, Anarcoma,… y revistas (El Rrollo Enmascarado, Star, La Piraña Divina, Butifarra!, Los Tebeos del Rrollo, Rock Comix, El Víbora,…, y mostrando su

  12. The Effect of Edaphic Factors on the Similarity of Parasitic Nematodes in the Soil Sampled in Nurseries of Ornamental Trees and Shrubs

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    Chałańska Aneta

    2014-09-01

    Full Text Available The largest faunistic similarity of nematodes was found in soils sampled in coniferous nurseries where arborvitae (Thuja occidentalis - Cupressaceae, spruces (Picea spp. - Pinaceae and pines (Pinus spp. - Pi-naceae were grown. In soil sampled from deciduous tree and shrub nurseries, similar species composition of parasitic nematodes was found in stands of oaks (Quercus spp. - Fagaceae, black locusts (Robiniapseudo-acacia - Fabaceae and maples (Acer spp. - Sapindaceae. In soils, especially the light and medium, from stands of coniferous and deciduous trees and shrubs, Aphelenchus avenae was often isolated. Bitylenchus dubius occurred in both types of nurseries, particularly in light soils. The largest faunistic similarities between nematodes isolated from places of growth of coniferous and deciduous plants were recorded in soils of loamy sand and sandy loam. The most abundant nematode species and the greatest similarity in species of plant parasitic nematodes were observed in soils with neutral pH or slightly acidic. Aphelenchus avenae was found in soil samples collected from both coniferous and deciduous plants, with no relation to soil acidity.

  13. A new genus with six new species of Typhlopolycystidinae Evdonin, 1977 (Platyhelminthes, Kalyptorhynchia, Polycystididae).

    Science.gov (United States)

    Schockaert, Ernest R; Martens, Paul M; Revis, Nathalie; Janssen, Toon; Willems, Wim; Artois, Tom J

    2014-01-22

    Five new species of the new taxon Brunetorhynchus n. gen. are described: B. deconincki n. sp., B. microstylis n. sp. and B. complicatus n. sp. are from the Mediterranean, B. canariensis n. sp. is from the Canary Island Lanzarote, B. cannoni n. sp. is from the Australian East coast and one species from the Galapagos, formerly described as Limipolycystis spec., is transferred to the new genus as B. dubius n. sp.. As in Limipolycystis, these species have a single stylet, (accessory stylet type II), an accessory secretion vesicle (type II) and a prostate vesicle (type III) in the male atrium, although the latter vesicle is absent in some species. Unlike the species of Limipolycystis, where the seminal receptacle is a sclerotized tubule, the species of the new taxon have a pear-shaped seminal receptacle on the oviduct. 

  14. Assessing risk to human health from tropical leafy vegetables grown on contaminated urban soils

    Energy Technology Data Exchange (ETDEWEB)

    Nabulo, G.; Young, S.D.; Black, C.R., E-mail: colin.black@nottingham.ac.uk [School of Biosciences, University of Nottingham, Nottingham, NG7 2RD (United Kingdom)

    2010-10-15

    Fifteen tropical leafy vegetable types were sampled from farmers' gardens situated on nine contaminated sites used to grow vegetables for commercial or subsistence consumption in and around Kampala City, Uganda. Trace metal concentrations in soils were highly variable and originated from irrigation with wastewater, effluent discharge from industry and dumping of solid waste. Metal concentrations in the edible shoots of vegetables also differed greatly between, and within, sites. Gynandropsis gynandra consistently accumulated the highest Cd, Pb and Cu concentrations, while Amaranthus dubius accumulated the highest Zn concentration. Cadmium uptake from soils with contrasting sources and severity of contamination was consistently lowest in Cucurbita maxima and Vigna unguiculata, suggesting these species were most able to restrict Cd uptake from contaminated soil. Concentrations of Pb and Cr were consistently greater in unwashed, than in washed, vegetables, in marked contrast to Cd, Ni and Zn. The risk to human health, expressed as a 'hazard quotient' (HQ{sub M}), was generally greatest for Cd, followed successively by Pb, Zn, Ni and Cu. Nevertheless, it was apparent that urban cultivation of leafy vegetables could be safely pursued on most sites, subject to site-specific assessment of soil metal burden, judicious choice of vegetable types and adoption of washing in clean water prior to cooking.

  15. Direct versus indirect climate controls on Holocene diatom assemblages in a sub-tropical deep, alpine lake (Lugu Hu, Yunnan, SW China)

    Science.gov (United States)

    Wang, Qian; Yang, Xiangdong; Anderson, Nicholas John; Dong, Xuhui

    2016-07-01

    The reconstruction of Holocene environmental changes in lakes on the plateau region of southwest China provides an understanding of how these ecosystems may respond to climate change. Fossil diatom assemblages were investigated from an 11,000-year lake sediment core from a deep, alpine lake (Lugu Hu) in southwest China, an area strongly influenced by the southwest (or the Indian) summer monsoon. Changes in diatom assemblage composition, notably the abundance of the two dominant planktonic species, Cyclotella rhomboideo-elliptica and Cyclostephanos dubius, reflect the effects of climate variability on nutrient dynamics, mediated via thermal stratification (internal nutrient cycling) and catchment-vegetation processes. Statistical analyses of the climate-diatom interactions highlight the strong effect of changing orbitally-induced solar radiation during the Holocene, presumably via its effect on the lake's thermal budget. In a partial redundancy analysis, climate (solar insolation) and proxies reflecting catchment process (pollen percentages, C/N ratio) were the most important drivers of diatom ecological change, showing the strong effects of climate-catchment-vegetation interactions on lake functioning. This diatom record reflects long-term ontogeny of the lake-catchment ecosystem and suggests that climatic changes (both temperature and precipitation) impact lake ecology indirectly through shifts in thermal stratification and catchment nutrient exports.

  16. Antioxidant and Anti-Inflammatory Activities of Kenyan Leafy Green Vegetables, Wild Fruits, and Medicinal Plants with Potential Relevance for Kwashiorkor

    Directory of Open Access Journals (Sweden)

    H. R. Tufts

    2015-01-01

    Full Text Available Background. Inflammation, together with related oxidative stress, is linked with the etiology of kwashiorkor, a form of severe acute malnutrition in children. A diet rich in anti-inflammatory and antioxidant phytochemicals may offer potential for the prevention and treatment of kwashiorkor. We selected and assayed five leafy green vegetables, two wild fruits, and six medicinal plants from Kenya for their antioxidant and anti-inflammatory properties. Consensus regarding medicinal plant use was established from ethnobotanical data. Methods. Antioxidant activity and phenolic content were determined using the oxygen radical absorbance capacity (ORAC assay and Folin-Ciocalteu procedure, respectively. Anti-inflammatory activity was assessed in vitro targeting the inflammatory mediator tumour necrosis factor-alpha (TNF-α. Results. Mangifera indica (leaves used medicinally showed the greatest antioxidant activity (5940 ± 632 µM TE/µg and total phenolic content (337 ± 3 mg GAE/g but Amaranthus dubius (leafy vegetable showed the greatest inhibition of TNF-α (IC50 = 9 ± 1 μg/mL, followed by Ocimum americanum (medicinal plant (IC50 = 16 ± 1 μg/mL. Informant consensus was significantly correlated with anti-inflammatory effects among active medicinal plants (r2=0.7639, P=0.0228. Conclusions. Several plant species commonly consumed by Kenyan children possess activity profiles relevant to the prevention and treatment of kwashiorkor and warrant further investigation.

  17. Pineal organs in deep demersal fish.

    Science.gov (United States)

    Wagner, H J; Mattheus, U

    2002-01-01

    We studied ten species of demersal fish from depths of 1500-4800 m, i.e. regions of the abyss outside the reach of sunlight. A pineal window in the skin and/or the skull, often found in mesopelagic fish, was never observed in demersal specimens. Nine species had a well-developed pineal organ, with light- and electron-microscopic features, well known in other teleosts living in surface waters, including photoreceptor cells with inner and outer segments, synaptic ribbons, neuronal perikarya, and (radial) glial cells. One species ( Bathypterois dubius) showed signs of regression; it also had reduced eyes. We observed considerable morphological variation in location, size, microscopic structure and ultrastructural organisation, including the frequency of photoreceptor cells, size of outer segments and the number of myelinated and unmyelinated axons. No systematic trend in the sense of an increase of sensitivity with greater depths was observed. Melatonin contents varied between 4 pg and 92 pg per pineal in the grenadier Coryphaenoides ( Nematonurus) armatus and between 2 pg and 70 pg per pineal in the eel Synaphobranchus kaupi. Differences between day and night values and between autumn and spring suggest that pineal melatonin acts as neurochemical signal mediating rhythmic processes and behaviour. The role of an alternative non-solar zeitgeber in the demersal environment is discussed.

  18. Seasonal and interannual variability in grey seal diets on Sable Island, eastern Scotian Shelf

    Directory of Open Access Journals (Sweden)

    W D Bowen

    2007-01-01

    Full Text Available We studied seasonal and interannual variability in the diet of grey seals (Halichoerus grypus using faecal samples collected from Sable Island, Nova Scotia between 1991 and 1998. More than 28,000 prey from at least 28 taxa were identified from 1,245 faecal samples collect mainly in spring, fall and winter. Sand lance (Ammodytes dubius dominated the diet in all seasons and years, but the importance of this and other species varied over time. There was also evidence of seasonal and interannual variation in the size of prey consumed both within and among species. We compared diet composition with estimates of prey numbers and biomass from annual researchtrawl surveys conducted in March and July. Species-specific numerical corrections were applied to otolith counts to account for the complete digestion of otoliths, and fish catchability correction factors applied to trawl survey catches to account for trawl selectivity. Based on an odds ratio index of prey selectivity, grey seals positively selected sand lance in both seasons. Other species were either relatively avoided or eaten roughly in proportion to their estimated abundance.

  19. Antioxidant and Anti-Inflammatory Activities of Kenyan Leafy Green Vegetables, Wild Fruits, and Medicinal Plants with Potential Relevance for Kwashiorkor.

    Science.gov (United States)

    Tufts, H R; Harris, C S; Bukania, Z N; Johns, T

    2015-01-01

    Background. Inflammation, together with related oxidative stress, is linked with the etiology of kwashiorkor, a form of severe acute malnutrition in children. A diet rich in anti-inflammatory and antioxidant phytochemicals may offer potential for the prevention and treatment of kwashiorkor. We selected and assayed five leafy green vegetables, two wild fruits, and six medicinal plants from Kenya for their antioxidant and anti-inflammatory properties. Consensus regarding medicinal plant use was established from ethnobotanical data. Methods. Antioxidant activity and phenolic content were determined using the oxygen radical absorbance capacity (ORAC) assay and Folin-Ciocalteu procedure, respectively. Anti-inflammatory activity was assessed in vitro targeting the inflammatory mediator tumour necrosis factor-alpha (TNF-α). Results. Mangifera indica (leaves used medicinally) showed the greatest antioxidant activity (5940 ± 632 µM TE/µg) and total phenolic content (337 ± 3 mg GAE/g) but Amaranthus dubius (leafy vegetable) showed the greatest inhibition of TNF-α (IC50 = 9 ± 1 μg/mL), followed by Ocimum americanum (medicinal plant) (IC50 = 16 ± 1 μg/mL). Informant consensus was significantly correlated with anti-inflammatory effects among active medicinal plants (r (2) = 0.7639,  P = 0.0228). Conclusions. Several plant species commonly consumed by Kenyan children possess activity profiles relevant to the prevention and treatment of kwashiorkor and warrant further investigation.

  20. Study on Phylogenetic Status of Javan Plover Bird (Charadrius, Charadriidae, Charadriiformes through DNA Barcoding Analysis

    Directory of Open Access Journals (Sweden)

    Hidayat Ashari

    2017-04-01

    Full Text Available Javan Plover named Charadrius javanicus is taxonomically under controversy and phylogenetically unresolved yet. Through an analysis of DNA barcode, this study aims (1 to confirm whether Javan Plover is separated species named Charadrius javanicus or a subspecies of C. alexandrinus which named C. a. javanicus and (2 to determine a relationship within this genus. Totally 666 bp DNA sequences of COI barcode gene were analyzed.  The results showed that a sequence divergence between Javan Plover and C. alexandrinus alexandrinus was only 1.2%, while sequence divergences between C.a.alexandrinus and others species, or between Javan Plover and others species were ranged from 9-12%.  Neighbour-joining (NJ and maximum-parsimony (MP analyses showed that all individuals of both Javan Plover and Kenith Plover were clustered together, and supported by 99 % and 100 % of bootstrap value in NJ and MP, respectively. This study tends to support the previous findings that Javan Plover was not a separated species named C. javanicus, but it was as a subspecies of C. alexandrinus; named C. a. javanicus. There were two groups of Plover in this study; (C. leschenaultii and C. javanicus + C.a.alexandrinus, and (C.dubius and C. melodus + C. semipalmatus. DNA barcoding analysis can give certainty taxonomic status of the bird. Then, this study has implication as a basic data that can be used to provide and support the planning of Javan plover conservation programs. 

  1. Screening of traditional South African leafy vegetables for specific anti-nutritional factors before and after processing

    Directory of Open Access Journals (Sweden)

    Humaira ESSACK

    Full Text Available Abstract This study investigated the effect of processing on anti-nutritional factors of thirteen traditional leafy vegetables collected in Kwa Zulu-Natal, South Africa. The aim was to determine whether processing reduced anti-nutrient levels of leafy vegetables. The vegetables were boiled in a plant-to-distilled water ratio of 1:4 (w/v at 97 °C for a time period of 5 and 15 min. The vegetables studied were: Amaranthus dubius, Amaranthus hybridus, Asystasia gangetica, Bidens pilosa, Ceratotheca triloba, Chenopodium album, Emex australis, Galinsoga parviflora, Guilleminea densa, Momordica balsamina, Oxygonum sinuatum, Physalis viscosa and Solanum nigrum. From this study, it was determined that non processed samples contained anti-nutrients such as tannins, phytic acid, alkaloids, oxalic acid, and cyanogenic glycoside. Both boiling parameters were effective in reducing the tannin, phytic acid, alkaloid, oxalic acid and cyanogenic glycoside contents of all 13 traditional leafy vegetables. The results of this study provide evidence that the local traditional leafy vegetables which the population is so reliant upon, are important contributors to micronutrient malnutrition in developing countries and can be minimized through common boiling methods for a minimum of 5 and maximum of 15 minutes.

  2. CUIDADO FAMILIAL DE RECÉM-NASCIDOS NO DOMICÍLIO: UM ESTUDO DE CASO ETNOGRÁFICO

    Directory of Open Access Journals (Sweden)

    Julliana de Souza Leandro

    2011-01-01

    Full Text Available El presente estudio de caso etnográfico es un estudio cualitativo con el objetivo de analizar la dinámica de la atención en el hogar de las familias de los recién nacidos en la ciudad de Piraí-RJ, Brasil. Se recogieron datos de 18 familias de los recién nacidos matriculados en la Unidad de Salud Familiar. Se emplearon los siguientes instrumentos: la entrevista no estructurada, los registros de los miembros de las familias en la Estrategia de Salud Familiar y la observación participante. El análisis temático reveló tres categorías: la dimensión psicológica del cuidado familiar al recién nacido en el hogar, la dimensión biosociocultural del cuidado familiar al recién nacido; y el cuidado familiar a los recién nacidos y su interfaz con el parto y el puerperio. En este estudio las dimensiones psicológicas y biosocioculturales de la atención familiar están presentes en diferentes espacios de la atención de la salud del recién nacido. Se concluye que en el cuidado de los recién nacidos y de la familia en su totalidad es necesario repensar las culturas que se entrelazan con el cuidado familiar y el cuidado profesional

  3. Avaliação de Métodos de Estimativa da Evapotranspiração de Referência para Fins de Manejo da Irrigação

    Directory of Open Access Journals (Sweden)

    Célia Maria Paiva

    2016-01-01

    Full Text Available O conhecimento da evapotranspiração das culturas é muito importante para o manejo da irrigação. A equação FAO56 Penman-Monteith tem sido recomendada pela FAO (Food and Agriculture Organization of the United Nations como método padrão para a estimativa da evapotranspiração de referência (ETo . Entretanto, esse método requer variáveis meteorológicas que em geral não são medidas nas estações meteorológicas no Brasil. Por isso, o objetivo deste estudo foi avaliar cinco métodos empíricos e comparar seus desempenhos com o de Penman-Monteith para as condições climáticas da região de Piraí do Sul no Estado do Paraná. Para tanto, foram utilizados dados meteorológicos registrados por uma plataforma de coleta de dados durante o período de 04/07/2008 a 12/10/2008 em uma cultura de trigo. Os resultados indicam que a ETo pode ser estimada adequadamente pelos métodos da Radiação Solar, Jensen-Haise e Makkink. O pior desempenho foi apresentado pelo método de Hargreaves-Samani, seguido do método de Camargo.

  4. Genetic diversity of tropical-adapted onion germplasm assessed by RAPD markers Diversidade genética em germoplasma tropical de cebola estimada via marcadores RAPD

    Directory of Open Access Journals (Sweden)

    Maria do Desterro M dos Santos

    2012-03-01

    Full Text Available Onion is a crop of significant socioeconomic importance to Brazil. Onion germplasm with adaptation to tropical and sub-tropical conditions has played an important role in the development of this crop in the country. In this context, we studied the genetic diversity in a germplasm collection potentially useful for the development of cultivars for tropical and subtropical regions. The genetic variability of 21 accessions/cultivars that have been used as germplasm and/or were developed by onion breeding programs in Brazil was evaluated via RAPD markers. The following accessions were included in the study :'Red Creole', 'Roxa IPA-3', 'Valenciana 14', 'Beta Cristal', 'Diamante', 'Composto IPA-6', 'Aurora', 'Bojuda Rio Grande', 'Alfa Tropical', 'Pêra IPA-4', 'Primavera', 'Belém IPA-9', 'Crioula Alto Vale', 'Conquista', 'Pira-Ouro', 'Vale-Ouro IPA-11', 'Franciscana IPA-10', 'Serrana', 'CNPH 6400', 'Petroline', and 'Baia Periforme'. From the 520 primers used in the initial screening only 38 displayed stable polymorphisms. They produced 624 amplicons, of which 522 (83.7% were monomorphic and 102 (16.3% were polymorphic. An average similarity coefficient of 0.72 was calculated among accessions based upon this subgroup of polymorphic amplicons. This allowed the discrimination of this germplasm collection into six groups with only one of them comprising more than one accession. The main group was formed by 16 accessions ('Diamante', 'Composto IPA-6', 'Aurora', 'Bojuda Rio Grande', 'Conquista', 'Pira-Ouro', 'Serrana', 'Vale-Ouro IPA-11', 'Baia Periforme', 'Primavera', 'Franciscana IPA-10', 'Belém IPA-9', 'Crioula Alto Vale', 'Petroline', 'Pêra IPA-4' and 'Alfa Tropical', for which the genetic origin (with few exceptions can be traced back to the variety 'Baia Periforme'. The populations 'Red Creole', 'Roxa IPA-3', 'Beta Cristal', 'CNPH 6400', and 'Valenciana 14' comprised a set of five isolated groups, showing genetic divergence among them and in

  5. Draft genome sequence of pathogenic bacteria Vibrio parahaemolyticus strain Ba94C2, associated with acute hepatopancreatic necrosis disease isolate from South America

    Directory of Open Access Journals (Sweden)

    Leda Restrepo

    2016-09-01

    Full Text Available Vibrio parahaemolyticus is a pathogenic bacteria which has been associated to the early mortality syndrome (EMS also known as hepatopancreatic necrosis disease (AHPND causing high mortality in shrimp farms. Pathogenic strains contain two homologous genes related to insecticidal toxin genes, PirA and PirB, these toxin genes are located on a plasmid contained within the bacteria. Genomic sequences have allowed the finding of two strains with a divergent structure related to the geographic region from where they were found. The isolates from the geographic collection of Southeast Asia and Mexico show variable regions on the plasmid genome, indicating that even though they are not alike they still conserve the toxin genes. In this paper, we report for the first time, a pathogenic V. parahaemolyticus strain in shrimp from South America that showed symptoms of AHPND. The genomic analysis revealed that this strain of V. parahaemolyticus found in South America appears to be more related to the Southeast Asia as compared to the Mexican strains. This finding is of major importance for the shrimp industry, especially in regards to the urgent need for disease control strategies to avoid large EMS outbreaks and economic loss, and to determine its dispersion in South America. The whole-genome shotgun project of V. parahaemolyticus strain Ba94C2 have been deposited at DDBJ/EMBL/GenBank under the accession PRJNA335761.

  6. 我国荷斯坦种公牛CVM遗传缺陷基因的分子检测%Identification of Complex Vertebral Malformation Carriers in Chinese Holstein Bulls

    Institute of Scientific and Technical Information of China (English)

    范学华; 张毅; 公维嘉; 谢岩; 孙东晓; 王雅春; 俞英; 张沅

    2010-01-01

    研究运用引入酶切位点聚合酶链式反应(PIRA-PCR)方法检测牛脊柱畸形综合症(CVM)在我国荷斯坦种公牛中的携带情况.共检测了来自全国14个公牛站的587头种公牛,结果表明:携带者56头,携带率为9.54%.系谱分析确认40头携带者公牛是Carlin-M Ivanhoe Bell的后代.对公牛信息统计显示,2001-2007年各年度出生的种公牛都保持较高的CVM携带率(8.22%~15.56%).美国、加拿大和澳大利亚是我国引进公牛的主要来源国,进口公牛的CVM携带率分别为11.37%、3.73%和14.29%.此外,本研究还对我国荷斯坦牛CVM遗传缺陷的控制和CVM携带公牛的利用提出建议.

  7. Trabalho rural e fatores de risco associados ao regime de uso de agrotóxicos em Minas Gerais, Brasil

    Directory of Open Access Journals (Sweden)

    Wagner Soares

    Full Text Available O objetivo deste artigo foi caracterizar o processo do trabalho rural em nove municípios de Minas Gerais, considerando indicadores sócio-demográficos, a estrutura agrária dos estabelecimentos rurais, práticas de trabalho relacionadas ao uso de agrotóxicos e, a intoxicação associada a seu uso. Os dados foram obtidos de uma pesquisa realizada pela Fundação Jorge Duprat Figueiredo, de Segurança e Medicina do Trabalho, que aplicou um questionário a 1.064 trabalhadores rurais, entre os anos de 1991 a 2000. Por meio de uma regressão logística, foram obtidos os fatores de risco associados à intoxicação por agrotóxicos dos grupos organofosforados e carbamatos. Cerca de 50% dos entrevistados se encontravam ao menos moderadamente intoxicados. Os fatores de risco encontrados foram: ter o último contato a menos de duas semanas com agrotóxicos; não usar proteção; ser orientado pelo vendedor; citar organofosforado ou carbamato como agrotóxico principal e trabalhar nos municípios de Teófoli Otoni, Guidoval ou Piraúba. Os resultados apontam para o alto grau de risco de agravos à saúde a que estão sujeitos trabalhadores rurais em contato com agrotóxicos.

  8. International conference centre, Geneva, Switzerland

    CERN Multimedia

    PH Department

    2009-01-01

    On 16-17 March 2009 the Energy Pact Foundation will be holding the Energy Pact Conference in Geneva. The Conference is organised with the support of the Republic and Canton of Geneva and has the Financial Times as its media partner. It will address for the first time in a comprehensive and integrated manner the key issues of energy needs and environmental and developmental challenges. Some 800 stakeholders and experts on these issues are expected. These will include high-level government officials, opinion leaders and representatives from the United Nations, NGOs, industry, civil society and the academic world. Gerhard Schröder, former Chancellor of Germany, will chair the Conference. Speakers with different backgrounds and expertise will include Dr. Carlo Rubia, Nobel Prize Winner, Ali Al-Naimi, Minister of Petroleum and Mineral Resources of Saudi Arabia, Gholam Hossein Nozari, Oil Minister of Iran, Gary Ross, CEO of PIRA Energy, a world-renowned energy market specialist, Ashok Khosla, President of the In...

  9. Trabalho rural e fatores de risco associados ao regime de uso de agrotóxicos em Minas Gerais, Brasil

    Directory of Open Access Journals (Sweden)

    Soares Wagner

    2003-01-01

    Full Text Available O objetivo deste artigo foi caracterizar o processo do trabalho rural em nove municípios de Minas Gerais, considerando indicadores sócio-demográficos, a estrutura agrária dos estabelecimentos rurais, práticas de trabalho relacionadas ao uso de agrotóxicos e, a intoxicação associada a seu uso. Os dados foram obtidos de uma pesquisa realizada pela Fundação Jorge Duprat Figueiredo, de Segurança e Medicina do Trabalho, que aplicou um questionário a 1.064 trabalhadores rurais, entre os anos de 1991 a 2000. Por meio de uma regressão logística, foram obtidos os fatores de risco associados à intoxicação por agrotóxicos dos grupos organofosforados e carbamatos. Cerca de 50% dos entrevistados se encontravam ao menos moderadamente intoxicados. Os fatores de risco encontrados foram: ter o último contato a menos de duas semanas com agrotóxicos; não usar proteção; ser orientado pelo vendedor; citar organofosforado ou carbamato como agrotóxico principal e trabalhar nos municípios de Teófoli Otoni, Guidoval ou Piraúba. Os resultados apontam para o alto grau de risco de agravos à saúde a que estão sujeitos trabalhadores rurais em contato com agrotóxicos.

  10. Electrical conductivity and emerging contaminant as markers of surface freshwater contamination by wastewater.

    Science.gov (United States)

    de Sousa, Diana Nara Ribeiro; Mozeto, Antonio Aparecido; Carneiro, Renato Lajarim; Fadini, Pedro Sergio

    2014-06-15

    The use of chemical markers of undoubted anthropogenic sources for surface freshwater contamination by wastewaters was evaluated employing correlations observed between measured physico-chemical parameters as the electrical conductivity and the concentration of different emerging organic compounds. During the period from April/2011 to April/2012 spatial-temporal variations and contamination patterns of two rivers (Piraí and Jundiaí rivers), São Paulo state, Brazil were evaluated. Seven physico-chemical parameters and concentrations of different classes of emerging contaminants were determined in samples collected in seven field campaigns. The high linear correlation coefficients obtained for the compounds diclofenac (r=0.9085), propanolol (r=0.8994), ibuprofen (r=0.8720) and atenolol (r=0.7811) with electrical conductivity, also corroborated by principal component analysis (PCA), point to the potential use of these compounds as markers of investigated surface water contamination by wastewaters. Due to specific inputs, these environmental markers showed very good effectiveness for the identification and differentiation of water body contamination by discharges of treated and untreated urban sewage.

  11. UTILIZAÇÃO DE RECURSOS ERGOGÊNICOS NUTRICIONAIS E/OU FARMACOLÓGICOS EM UMA ACADEMIA DA CIDADE DE BARRA DO PIRAÍ, RJ

    Directory of Open Access Journals (Sweden)

    Luana Palmeira Pereira

    2014-02-01

    Full Text Available The habit of doing workouts combined with use of nutritional ergogenic or pharmacological has grown considerably in the last years. Whether for esthetic reasons or in order to improve some performance aspects, athletes consume a large amount of these products. In this sense, the purpose of this study is: survey the consumers profile of a nutritional ergogenic resources in Barra do Piraí gyms (RJ state, enumerate the products used by users of both sexes and verify the prescription source of these products to researched participants, ascertain if the participants achieved the expected results with use of these products and if they caused any side effects. This is an observational study, which evaluated 101 athletes of both genders and social classes. It was found that the majority of participants 75% (n = 76 reported the use of nutritional ergogenic or pharmacological, with the highest prevalence among men. Two products were the most consumed: whey protein 56.57% (n = 34 and creatine 42.10% (n = 25. The majority 45% (n = 34 reported consuming ergogenic without an expert guidance. Therefore, that the study shows the importance of each time more the professional in the field of nutrition be inserted in the sports ground for possible explanations about the effects of this product type is found between sportsman.

  12. Systems Biology Strategy Reveals PKC-delta is Key for Sensitizing TRAIL-Resistant Human Fibrosarcoma

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    Kentaro eHayashi

    2015-01-01

    Full Text Available Cancer cells are highly variable and resistant to therapeutic intervention. Recently, the use of the tumor necrosis factor related apoptosis-inducing ligand (TRAIL induced treatment is gaining momentum, due to TRAIL’s ability to specifically target cancers with limited effect on normal cells. However, several malignant cancer types still remain non-sensitive to TRAIL. Previously, we developed a dynamic computational model, based on perturbation-response approach, and predicted protein kinase C (PKC as the most effective target, with over 95% capacity to kill human fibrosarcoma (HT1080 in TRAIL stimulation (Piras, V. et al. 2011, Scientific Reports. Here, to validate the model prediction, which has significant implications for cancer treatment, we conducted experiments on two TRAIL-resistant cancer cell lines (HT1080 and HT29. Using PKC inhibitor Bisindolylmaleimide I, we first demonstrate, as predicted by our previous model, cell viability is significantly impaired with over 95% death of both cancer types. Next, to identify crucial PKC isoform from 10 known members, we analyzed their mRNA expressions in HT1080 cells and shortlisted 4 isoforms for siRNA knock-down (KD experiments. From these KDs, PKC-delta produced the most cancer cell death in conjunction with TRAIL. Overall, systems biology approach, combining model prediction with experimental validation, holds promise for TRAIL-based cancer therapy.

  13. Draft genome sequence of pathogenic bacteria Vibrio parahaemolyticus strain Ba94C2, associated with acute hepatopancreatic necrosis disease isolate from South America.

    Science.gov (United States)

    Restrepo, Leda; Bayot, Bonny; Betancourt, Irma; Pinzón, Andres

    2016-09-01

    Vibrio parahaemolyticus is a pathogenic bacteria which has been associated to the early mortality syndrome (EMS) also known as hepatopancreatic necrosis disease (AHPND) causing high mortality in shrimp farms. Pathogenic strains contain two homologous genes related to insecticidal toxin genes, PirA and PirB, these toxin genes are located on a plasmid contained within the bacteria. Genomic sequences have allowed the finding of two strains with a divergent structure related to the geographic region from where they were found. The isolates from the geographic collection of Southeast Asia and Mexico show variable regions on the plasmid genome, indicating that even though they are not alike they still conserve the toxin genes. In this paper, we report for the first time, a pathogenic V. parahaemolyticus strain in shrimp from South America that showed symptoms of AHPND. The genomic analysis revealed that this strain of V. parahaemolyticus found in South America appears to be more related to the Southeast Asia as compared to the Mexican strains. This finding is of major importance for the shrimp industry, especially in regards to the urgent need for disease control strategies to avoid large EMS outbreaks and economic loss, and to determine its dispersion in South America. The whole-genome shotgun project of V. parahaemolyticus strain Ba94C2 have been deposited at DDBJ/EMBL/GenBank under the accession PRJNA335761.

  14. Capitalismo y organización de los espacios ganaderos en España. El caso de la Cooperativa Agropecuaria de Guissona

    Directory of Open Access Journals (Sweden)

    José Antonio Segrelles Serrano

    1999-01-01

    Full Text Available El proceso de intensificación productiva de la ganadería espaflola se relaciona directamente con la penetración del sistema capitalista en el sector agropecuario. Las necesidades y exigencias de este modo de producción tienen una dimensión espacial que se refleja en la forma en que se produce la apropiación, gestión y organización del territorio por parte de las empresas más poderosas del sector, cuyos centros de trabajo y producción se diseminan en el espacio para realizar complejos procesos productivos que generan plusvaiias y una posterior acumulación del capital en sus sedes metropolitanas. Cataluña ha sido la región espaflola pionera en la adopción de métodos intensivos y capitalistas en la ganadería. lo que permitió una acumulación de capital más antigua y eficaz. Desde el área metrooolitana de Barcelona v sectores próximos. muchas empresas. autóctonas y extranjeras. timan las decisiones inversoras que serán determinantes pira la creación, transformación y control de los espacios agropecuarios. tanto catalanes como del resto del Estado.

  15. Photorhabdus luminescens PirAB-fusion protein exhibits both cytotoxicity and insecticidal activity.

    Science.gov (United States)

    Li, Yusheng; Hu, Xiaofeng; Zhang, Xu; Liu, Zhengqiang; Ding, Xuezhi; Xia, Liqiu; Hu, Shengbiao

    2014-07-01

    The binary toxin 'Photorhabdus insect-related' proteins (PirAB) produced by Photorhabdus luminescens have been reported to possess both injectable and oral activities against a range of insects. Here, PirAB-fusion protein was constructed by linking pirA and pirB genes with the flexible linker (Gly4 Ser)3 DNA encoding sequence and then efficiently expressed in Escherichia coli. To better understand the role of PirAB toxin played in the process of invasion, its cytotoxicity against insect midgut CF-203 cells was investigated. Application of purified PirAB-fusion protein as well as PirA/PirB mixture caused loss of viability of CF-203 cells after 24 h incubation. CF-203 cells treated by PirAB-fusion protein displayed morphological changes typical of apoptosis, such as cell shrinkage, cell membrane blebbing, nuclear condensation and DNA fragmentation. Moreover, PirAB-fusion protein also exhibited injectable insecticidal activity against Spodoptera exigua larvae. The bodies of S. exigua fourth-instar larvae injected with PirAB-fusion protein turned completely black. Thus, we concluded that PirAB-fusion protein possessed similar biological activity (cytotoxicity and insecticidal activity) to PirA/PirB mixture, which would enable it to be used as an efficient agent for pest control.

  16. Identidade, riqueza e abundãncia de pequenos mamíferos (Rodentia e Didelphimorphia de área de Floresta com Araucária no estado do Paraná, Brasil

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    Guilherme Grazzini

    2015-01-01

    Full Text Available Roedores e Marsupiais são componentes importantes da fauna na região Neotropical. Estudos sobre a riqueza e abundância destes animais são raros na Floresta Ombrófila Mista (FOM e aqui apresentamos um inventário conduzido na Floresta Nacional de Piraí do Sul. Esta área possui uma área aproximada de 150 ha, cercada por pastos e plantações de Pinus. A amostragem foi feita através de armadilhas de queda, Sherman e Tomahawk, dispostas nos principais tipos de vegetação encontrados. Pelo menos um casal de cada espécie foi coletado. Como resultado, durante 5.892 armadilhas.noite nós obtivemos um total de 1.049 capturas, representando 17 espécies: Akodon montensis, Bibimys labiosus, Brucepattersonius iheringi, Cryptonanus sp., Didelphis albiventris, D. aurita, Gracilinanus microtarsus, Monodelphis americana, M. scalops, Myocastor coypus, Nectomys squamipes, Oligoryzomys nigripes, Oxymycterus judex, O. nasutus, Sooretamys angouya e Thaptomys nigrita. Capturas por armadilhas de queda representaram 67% do total, e oito espécies foram registradas exclusivamente por este método. Akodon montensis, O. nigripes e T. nigrita foram as espécies mais abundantes.

  17. New Eocene Coleoid (Cephalopoda Diversity from Statolith Remains: Taxonomic Assignation, Fossil Record Analysis, and New Data for Calibrating Molecular Phylogenies.

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    Pascal Neige

    Full Text Available New coleoid cephalopods are described from statolith remains from the Middle Eocene (Middle Lutetian of the Paris Basin. Fifteen fossil statoliths are identified and assigned to the Sepiidae (Sepia boletzkyi sp. nov.,? Sepia pira sp. nov., Loliginidae (Loligo clarkei sp. nov., and Ommastrephidae (genus indet. families. The sediments containing these fossils indicate permanent aquatic settings in the infralittoral domain. These sediments range in age from 46 Mya to 43 Mya. Analysis of the fossil record of statoliths (from findings described here, together with a review of previously published data indicates marked biases in our knowledge. Fossil statoliths are known from as far back as the Early Jurassic (199.3 to 190.8 Mya but surprisingly, to the best of our knowledge, no record occurs in the Cretaceous. This is a "knowledge bias" and clearly calls for further studies. Finally, we attempt to compare findings described here with fossils previously used to constrain divergence and/or diversification ages of some coleoid subclades in molecular phylogenies. This comparison clearly indicates that the new records detailed here will challenge some estimated divergence times of coleoid cephalopod subclades.

  18. Molecular identification and transmission studies of X-cell parasites from Atlantic cod Gadus morhua (Gadiformes: Gadidae and the northern black flounder Pseudopleuronectes obscurus (Pleuronectiformes: Pleuronectidae

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    Watanabe K

    2011-02-01

    Full Text Available Abstract Background Epidermal pseudotumours from Hippoglossoides dubius and Acanthogobius flavimanus in Japan and gill lesions in Limanda limanda from the UK have been shown to be caused by phylogenetically related protozoan parasites, known collectively as X-cells. However, the phylogenetic position of the X-cell group is not well supported within any of the existing protozoan phyla and they are currently thought to be members of the Alveolata. Ultrastructural features of X-cells in fish pseudotumours are somewhat limited and no typical environmental stages, such as spores or flagellated cells, have been observed. The life cycles for these parasites have not been demonstrated and it remains unknown how transmission to a new host occurs. In the present study, pseudobranchial pseudotumours from Atlantic cod, Gadus morhua, in Iceland and epidermal pseudotumours from the northern black flounder, Pseudopleuronectes obscurus, in Japan were used in experimental transmission studies to establish whether direct transmission of the parasite is achievable. In addition, X-cells from Atlantic cod were sequenced to confirm whether they are phylogenetically related to other X-cells and epidermal pseudotumours from the northern black flounder were analysed to establish whether the same parasite is responsible for infecting different flatfish species in Japan. Results Phylogenetic analyses of small subunit ribosomal DNA (SSU rDNA sequence data from Atlantic cod X-cells show that they are a related parasite that occupies a basal position to the clade containing other X-cell parasites. The X-cell parasite causing epidermal pseudotumours in P. obscurus is the same parasite that causes pseudotumours in H. dubius. Direct, fish to fish, transmission of the X-cell parasites used in this study, via oral feeding or injection, was not achieved. Non-amoeboid X-cells are contained within discrete sac-like structures that are loosely attached to epidermal pseudotumours in

  19. Ocorrência de Commelina villosa como planta daninha em áreas agrícolas no estado do Paraná-PR, Brasil Occurence of Commelina villosa as weed in Paraná State, Brazil

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    Dalva C. Rocha

    2000-01-01

    Full Text Available As trapoerabas pertencem à família Commelinaceae e são plantas daninhas de difícil controle em diferentes regiões do país. No Brasil, a espécie Commelina benghalensis destaca-se como a principal trapoeraba infestante nas culturas de soja, milho, café e citros. Outras duas espécies desse gênero, Commelina diffusa e Commelina erecta, também são conhecidas como infestantes de ocorrência freqüente no território nacional. Commelina villosa está registrada, até o momento, apenas para os Estados da Bahia e Goiás além do Distrito Federal. O presente trabalho teve como objetivos caracterizar e registrar a ocorrência de C. villosa no Estado do Paraná, onde pode estar sendo confundida com outras trapoerabas, principalmente C. benghalensis. Exemplares de C. villosa e C. benghalensis foram coletados, de forma aleatória, em lavouras de soja, feijão e milho, nos municípios de Ponta Grossa, Tibagi, Piraí do Sul, Guarapuava, Pato Branco, Francisco Beltrão, Cascavel, Campo Mourão e Londrina. Parte desse material foi herborizado para a confecção de exsicatas e outra parte foi mantida in vivo, cultivada no Departamento de Botânica, do Instituto de Biociências de Botucatu-UNESP. Caracteres morfológicos descritivos e quantitativos foram avaliados e as espécies comparadas entre si. C. villosa distinguiu-se de C. benghalensis por apresentar folhas maiores (9,76 x 3,26 cm, elíptica a elípticaestreita, sésseis, de coloração verde escura com manchas violáceas na face inferior, filetes translúcidos, entre outras características. A ocorrência de C. villosa no Paraná foi constatada em todos os municípios amostrados, com exceção de Campo Mourão e Londrina.Commelinaceae are weeds with unsatisfactory control in different parts of the country. Commelina benghalensis is the main Commelinaceae weed in soybean, corn, coffee and orange crops in Brazil. Commelina diffusa and Commelina erecta are also known as common weeds in national

  20. Characterization of native and oxidized starches of two varieties of Peruvian carrot (Arracacia xanthorrhiza, B. from two production areas of Paraná state, Brazil

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    Luciana Shizue Matsuguma

    2009-06-01

    Full Text Available Two commercial varieties of Peruvian carrot ('Amarela de Carandaí' and 'Senador Amaral' were processed into flour, starch and bagasse and chemically evaluated. The starch was extracted, modified with H2O2 and characterized by the physicochemical methods. By using the methylene blue dyeing, the granules of the modified starches showed intense blue color. The carboxyl content, the reducing power and the amount of the water liberated from the pastes after the freeze-thawing were higher for the oxidized starches and their pastes were clearer than those of the native starches of the two varieties from the two production areas. The RVA viscoamylography showed that the modified starches had lower viscosities with differences between the varieties. In the thermal analysis, the temperatures of the pyrolysis were higher for the native (310.37, 299.08, 311.18 ºC than for the modified starches (294.16, 296.65 e 293.29 ºC for both the varieties. This difference could be related with the larger surface of the granules due to the partial degradation promoted by the chemical modification. In almost all results, the differences were evident between the varieties but not for the cultivation places.No presente trabalho duas variedades comerciais de mandioquinha-salsa ('Amarela de Carandaí' e 'Senador Amaral' obtidas dos municípios de Castro e de Piraí do Sul (Paraná foram processadas para a obtenção de farinha, farelo e amido e essas três frações foram caracterizadas quimicamente. O amido foi extraído, modificado com H2O2 e caracterizado por meio de análises físico-químicas. Na coloração com azul de metileno os grânulos dos amidos oxidados apresentaram coloração azul mais intensa. O teor de carboxilas, o poder redutor e a quantidade de água liberada das pastas foram maiores para as amostras de amidos modificados. As pastas dos amidos modificados foram mais claras que as dos nativos. Na análise viscoamilográfica (RVA os amidos modificados

  1. Utilização de análise multivariada e redes neurais artificiais na determinação do comportamento de colonização de populações de macrófitas aquáticas no reservatório de Santana Using multivariate statistics and artificial neural networks to determe the colonization behavior of aquatic macrophyte populations in Santana reservoir

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    R.L.C.M. Pitelli

    2009-01-01

    Full Text Available As análises de agrupamento e de componentes principais e as redes neurais artificiais foram utilizadas na determinação de padrões de comportamento das populações de macrófitas aquáticas que colonizaram o reservatório de Santana, Piraí-RJ, durante o ano de 2004. As análises de agrupamento dividiram o comportamento das populações durante o ano em dois grupos distintos, apresentando um padrão no primeiro semestre que difere daquele observado no segundo semestre do ano. A análise de componentes principais demonstrou que esse comportamento da comunidade (grupo de populações é influenciado principalmente pelas espécies S. montevidensis, Heteranthera reniformis, Ludwigia sp., Rhynchospora aurea, C. iria, C. ferax e Aeschynomene denticulata no primeiro grupo e por Echinochloa polystachya, Polygonum lapathifolium, Alternanthera phyloxeroides, Pistia stratiotes, Eichhornia azurea, Brachiaria arrecta e Oxyscarium cubense no segundo grupo. As redes neurais artificiais agruparam as populações de macrófitas aquáticas em nove grupos, conforme sua densidade nos diferentes meses do ano. A aplicação da análise de componentes principais (ACP nos valores de frequência das populações presentes nos primeiros três grupos de Kohonen permitiu discriminar três grupos de meses, cujas populações apresentaram características diferentes de colonização. A aplicação das redes neurais artificiais permitiu melhor discriminação dos meses e das espécies que compõem as comunidades correspondentes, quando utilizada a análise de componentes principais.Cluster analysis, principal components analysis and Kohonen artificial neural networks were tested to determine the behavior patterns of aquatic macrophyte colonization on Santana reservoir, Piraí-RJ, 2004. The colonization behavior of the populations was divided into two groups, representing the two year semesters. The principal components analysis showed that the behavior of the first group

  2. The relationship between single nucleotide polymorphisms of 1154G/A in VEGF gene and the onset risk of lung cancer of Han people population in north China%VEGF基因-1154G/A单核苷酸多态性与中国北方汉族人群肺癌发病风险的关系

    Institute of Scientific and Technical Information of China (English)

    高峰; 周雪涛; 张泽峰; 郭阳

    2011-01-01

    目的 探讨VEGF基因启动子区-1154G/A单核苷酸多态性与中国北方汉族人群肺癌发病风险的关系.方法 采用基于医院的病例-对照研究方法,采集200例肺癌患者和204例健康对照个体的静脉抗凝血,同时记录其病史和个人相关资料.以蛋白酶K消化-饱和氯化钠盐析法提取外周血白细胞DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法和引物介导的限制性聚合酶链反应(PIRA-PCR)方法检测VEGF-1154 G/A多态性位点的基因型.结果 VEGF-1154 G/A基因型和等位基因频率分布在肺癌组与健康对照组之间差异均无统计学意义(P>0.05).与G/G基因型相比,携带A等位基因的基因型(G/A+ A/A),可降低不吸烟个体肺癌的发病风险.未发现VEGF-1154 G/A多态性与肺鳞状细胞癌、肺腺癌及小细胞肺癌的发病风险相关.结论 VEGF-1154 G/A单核苷酸多态性可能与不吸烟个体肺癌发病风险相关.即携带A等位基因的基因型( G/A+ A/A),可降低不吸烟个体肺癌的发病风险.%Objective To investigate the correlation between single nucleotide polymorphisms (SNP) of-1154G/A in VEGF gene and the onset risk of lung cancer of Han people population in north China. Methods The case-control study based on hospital was used,and the venous anticoagulated blood specimens of 200 patients with lung cancer and 204 healthy subjects were collected, meanwhile, the patients' medical history and the data about the healthy subjects were recorded. The genomic DNA was extracted by using proteinase K digestion followed by a salting out procedure. Polymorphisms of VEGF gene were analyzed by PCR-restriction fragment length polymorphism analysis ( PCR-RFLP) and primer-introduced restriction analysis PCR (PIRA-PCR). Results There was no significant difference in the genotype of 1154G/A in VEGF and allele gene frequency distribution between lung cancer group and control group ( P >0.05). As compared with G/G genotype

  3. Variabilidade genética e sensibilidade de acessos de Pistia stratiotes ao herbicida glyphosate Genetic variability and sensitivity of Pistia stratiotes accesses to glyphosate

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    E.A.S. Cícero

    2007-09-01

    Full Text Available A alface-d'água (Pistia stratiotes é uma das principais entre as macrófitas aquáticas que causam problemas em corpos hídricos no Brasil e são consideradas como plantas daninhas. O presente trabalho foi realizado com os objetivos de conhecer melhor a variabilidade genética dessa macrófita e relacionar essa variabilidade com a resposta à aplicação do herbicida glyphosate. Para isso, foram coletados indivíduos em 12 corpos hídricos em diferentes cidades do território nacional (Americana, Cambaratiba, Curitiba, Itapura, Jaboticabal, Lagoa Santa, Piraí, Rio Grande, Rubinéia, Salto Grande, Santa Gertrudes e Três Lagoas. Os acessos foram caracterizados pelo uso de marcadores RAPD (DNA Polimórfico Amplificado ao Acaso, que permitiram, com o auxílio de iniciadores aleatórios, a caracterização dos locos polimórficos identificados por uma matriz de ausência e presença de bandas. Utilizando essa matriz, a análise de agrupamento permitiu nítida classificação dos acessos em três grupos com diferenças genéticas entre eles. Um ensaio de controle químico, com plantas mantidas em vasos plásticos (5 L e pulverizadas com o herbicida glyphosate nas concentrações de 0,0, 0,6, 1,2, 1,8 e 2,4 kg ha-1, identificou, utilizando avaliações aos 7, 14 e 21 dias após aplicação, que as duas maiores doses promoveram melhor efeito herbicida. Foi verificado também que os acessos de Curitiba e Cambaratiba apresentaram menor suscetibilidade ao herbicida glyphosate. Não houve correspondência entre a estrutura de grupos dos acessos pela análise multivariada de agrupamento com a técnica RAPD e a suscetibilidade da alface-d'água ao glyphosate.Water lettuce (Pistia stratiotes is one the most important macrophytes, classified as weed and causing serious problems in watercourses in Brazil. The aim of this research was to evaluate the genetic variability of water lettuce and its relationship with this plant's susceptibility to glyphosate

  4. Controle pós-emergente de plantas daninhas em cenoura Post-emergence weed control in carrot crop

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    Jeferson Zagonel

    1999-03-01

    Full Text Available O experimento foi conduzido em Piraí do Sul-PR no ano de 1995 em solo de textura argilo arenosa, tendo como objetivo avaliar a eficiência e a seletividade do herbicida propaquizafop no controle pós-emergente de plantas daninhas na cultura da cenoura. O delineamento experimental utilizado foi de blocos ao acaso com seis tratamentos e quatro repetições, quais sejam: propaquizafop (100, 125 e 150 g i.a./ha; fluazifop-p-butil (188 g i.a./ha; testemunha capinada e testemunha sem capina. A cultivar de cenoura utilizada foi Nantes Tim Tom semeada em 15 de junho, com espaçamento de 0,25 x 0,05 m, em parcelas com área útil de 5,00 x 1,00 m. As plantas daninhas predominantes foram Brachiaria plantaginea (capim-papuã, Digitaria horizontalis (capim-milhã e Eleusine indica (capim pé-de-galinha. As avaliações foram efetuadas aos 15, 30 e 45 dias após a aplicação dos tratamentos. Observou-se que o herbicida propaquizafop, nas doses de 100, 125 e 150 g i.a./ha foi eficiente no controle sobre capim-papuã, capim-milhã e capim pé-de-galinha. O controle de plantas daninhas realizado através de herbicidas proporcionou a mesma produção obtida com o controle manual. A perda na produção ocasionada pela presença de plantas daninhas foi da ordem de 76,4%. Não foram observados efeitos fitotóxicos nas plantas de cenoura que pudessem ser atribuídos aos produtos utilizados.The presented field trial was conducted in 1995 in Piraí do Sul, Paraná State, Brazil, on a sand-clay texture soil, to evaluate the efficiency and selectivity of propaquizafop on carrot crop weed control. The experiment was laid out in a randomized block design with six treatments and four replications, and 5.00 x 1.00 m plots. The treatmens were: propaquizafop (100, 125 and 150 g a.i/ha; fluazifop-p-butil (188 g a.i/ha; control with weeding and control without weeding. The carrot cultivar Nantes Tim Tom was sown on June 15 with plants spaced 0.25 x 0.05 m. The prevalent weeds

  5. Suscetibilidade à queda natural e caracterização dos frutos de diversos genótipos de bananeiras Different banana genotypes in relation to their susceptibility to finger drop and fruit characterization

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    Marlon Cristian Toledo Pereira

    2004-12-01

    Full Text Available A queda natural de frutos maduros da bananeira, resultado da separação individual de frutos da coroa da penca, também chamada despencamento, é uma característica indesejável, que pode limitar o lançamento de uma nova cultivar. O fruto destacado da penca tem vida de prateleira reduzida, além de não demonstrar boa aparência aos olhos do consumidor. Os objetivos do presente trabalho foram quantificar a suscetibilidade à queda natural dos frutos de bananeiras de grupos genômicos e ploidias diferentes, e identificar correlações entre a queda natural e diversas características físicas dos frutos. Foram utilizados 37 genótipos de bananeiras. De acordo com análise de variância e teste de Scott-Knott, os resultados evidenciaram a alta resistência ao despencamento dos genótipos pertencentes ao grupo genômico BB (Butuhan, Piraí e BB França, Terra (AAB, Poteau Nain (tipo figo (ABB e Thap Maeo (AAB, enquanto Prata-Anã (AAB, Grande Naine (AAA, Ambrósia (AAAA, Ouro (AA e FHIA 18 (AAAB obtiveram valores intermediários de resistência ao despencamento. Com relação às bananeiras suscetíveis, destacam-se os híbridos melhorados Pioneira (AAAB, YB42-21 (AAAB, Buccaneer (AAAA e Calypso (AAAA e a cultivar Ouro da Mata (AAAB. Verificou-se associação de 74% entre a firmeza do fruto e a resistência ao despencamento. Os estudos de grupos genômicos e ploidias indicaram maior resistência ao despencamento das bananeiras pertencentes ao grupo BB e dos genótipos triplóides ABB e AAB.Natural fruit dropping of ripe bananas in result of individual separation of fruits from the hand crown, is called finger drop, this characteristic is undesirable and would limit the marketing of new cultivars. Fruits that stand out in the hand have a reduced shelf life, and do not present a pleasant appearance for customers. This study was carried out in order to quantify the susceptibility to natural fruit dropping in fruits from different genomic groups and

  6. Desempenho de cultivares de cebola em cultivo orgânico e tipos de solo no Vale do São Francisco Evaluation of onion cultivars under organic cultivation in two soil types in the São Francisco Valley, Brazil

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    Nivaldo D Costa

    2008-12-01

    Full Text Available Com o objetivo de avaliar a produtividade de cultivares de cebola em cultivo orgânico no Vale do São Francisco, conduziram-se dois experimentos, de maio a outubro de 2005, nos Campos Experimentais de Bebedouro, Petrolina-PE, e Mandacaru, Juazeiro-BA, em ARGISSOLO e VERTISSOLO respectivamente. O delineamento experimental utilizado foi em blocos ao acaso, utilizando-se dezoito e quatorze cultivares de cebola, respectivamente e quatro repetições. Em ARGISSOLO a produtividade total de bulbos variou de 13,52 a 39,52 t/ha. A produtividade comercial oscilou de 7,45 a 38,32 t/ha, sobressaindo-se como mais produtivas as cultivares Brisa IPA-12 (38,32 t/ha e São Paulo (35,86 t/ha que não evidenciaram diferenças significativas entre si, assim como as cultivares Botucatu-150 (26,41 t/ha e Pira Ouro (26,37 t/ha, e menos produtivas as cultivares Conquista (7,45 t/ha e Crioula Alto Vale (7,81 t/ha. Em VERTISSOLO a produtividade total de bulbos variou de 6,87 a 24,68 t/ha. Sobressairam-se com produtividade comercial as cultivares Texas Grano PRR (21,56 t/ha e IPA-10 (17,50 t/ha, que não diferiram entre si. As cultivares Crioula Alto Vale, CNPH-6348, CNPH- 6436 e CNPH-6206 não tiveram produção comercial de bulbos.In this research were evaluated the yield of onion cultivars under organic growth in the São Francisco Valley. Two field trials were evaluated, one at the Experimental Farm Station of Embrapa Tropical Semi-Arid, in Petrolina, in an ultisol type and the other at the Experimental Farm Station of Mandacaru, in Juazeiro, in a vertisol type, from February to October of 2005. The experimental design was of randomized complete blocks, with 18 and 14 cultivars, respectively, and four replications. In the ultisol, the total yield of bulbs ranged from 13.52 to 39.52 t/ha. The commercial yield ranged from 7.45 to 38.32 t/ha where the cultivars Brisa (38.32 t/ha and São Paulo (35.86 t/ha showed the highest yield without statistical difference

  7. 天津地区荷斯坦公牛CVM、DUMPS和CN遗传缺陷检测%Identiifcation of CVM, DUMPS and CN Carriers in Chinese Holstein Bulls in Tianjin Area

    Institute of Scientific and Technical Information of China (English)

    梁若冰; 谢岩; 范学华; 张毅; 孙东晓; 张胜利; 马毅; 吴周良

    2014-01-01

    本文旨在研究天津地区中国荷斯坦公牛脊椎畸形综合征(Complexvertebralmalformation,CVM)、尿苷酸合酶缺乏症(Deficiencyofuridinemonophosphatesynthase,DUMPS)和瓜氨酸血症(Citrullinemia,CN)3种遗传缺陷的携带者比率及系谱来源。通过PIRA-PCR和PCR-RFLP方法分别对天津奶牛发展中心参加全国青年公牛联合后裔测定和国家良种补贴项目的110头荷斯坦公牛进行了CVM、DUMPS和CN三种遗传缺陷检测。共发现6头CVM隐性有害基因携带公牛,携带者比例为5.45%,隐性有害等位基因频率为2.72%。经过系谱分析,其中4头CVM携带者均为美国公牛Carlin-MIvanhoeBell的后代,另外2头因系谱不完整而无法查询。未检测到DUMPS和CN隐性有害基因携带者。基于此,我国有必要尽快建立荷斯坦牛隐性遗传缺陷监控体系并进行系谱标注,避免携带公牛进入后裔测定和良种补贴项目,以逐步降低我国奶牛群体中遗传缺陷隐性等位基因频率。%This study is aimed to detect carriers of Complex vertebral malformation (CVM), deficiency of uridine monophosphate synthase (DUMPS) and Citrullinemia (CN) in Chinese Holstein bulls in Tianjin area. Polymerase chain reaction-primer introduced restriction analysis (PIRA-PCR) and restriction fragment length polymorphism (PCR-RFLP) methods were used to detect the alleles of CVM, DUMPS and CN among 110 Holstein bullsparticipating in the national progeny test bulls program. As a result, a total of 6 CVM carriers were identiifed. The recessive allele frequency of CVM is 5.45%. After pedigree analysis, out of 6 CVM carriers, 4 bulls are found to the offspring of American Holstein bull“Carlin-M Ivanhoe Bell”, and 2 bulls’ pedigrees are incomplete. DUMPS and CN carrier didn’t be identiifed. Based on this, in our country, it is necessary to establish as soon as possible Holstein recessive genetic defect monitoring system and conduct genealogy

  8. A influência da Ação Católica na atuação social e pastoral de D. Waldyr Calheiros: o caso dos Direitos Humanos na cidade Volta Redonda * La influencia de la Acción Catolica...

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    LUIZ FERNANDO MANGEA DA SILVA

    2014-09-01

    Full Text Available Resumo: O presente artigo faz uma análise da influência da Ação Católica na atuação social e pastoral de D. Waldyr Calheiros de Novaes na Diocese de Barra do Piraí/Volta Redonda, tendo como base o contexto sociopolítico nacional e local do início da década de 1950 até 1968. Essa atuação se inicia a partir do episódio referente à violação dos direitos humanos, em que operários da Companhia Siderúrgica Nacional e intelectuais volta-redondenses foram presos após uma comemoração pelos 20 anos da Declaração Universal dos Direitos do Homem. No evento, também foram comemorados os dois anos da chegada e posse do bispo à diocese. Houve, por parte da organização do evento, a iniciativa de incluir dramatizações que simbolizavam o universo dos trabalhadores. Não podemos esquecer que Volta Redonda era uma cidade militarizada, por causa da instalação do 1º Batalhão de Infantaria Blindada em Barra Mansa, cidade vizinha. O serviço de espionagem do regime militar identificou as lideranças operárias e intelectuais, o que resultou nas prisões dos organizadores do evento. Foi nesse episódio que o bispo se declarou preso no batalhão, em protesto contra a detenção dessas lideranças.Palavras-chave: Waldyr Calheiros de Novaes – Ação Católica – Ditadura Militar e Direitos Humanos. Resumen: Este artículo analiza la influencia de la Acción Católica en el trabajo social y pastoral D. Waldyr Calheiros de Novaes en la Diócesis de Barra do Piraí/Volta Redonda, en función del contexto socio-político nacional y local de la década de 1950 hasta 1968 . Dicha acción se inicia desde el episodio relativo a la violación de los derechos humanos, en el que los trabajadores nacionales Steel Company y los intelectuales era redondenses fueron arrestados después de una celebración para el 20 aniversario de la Declaración universal de los derechos humanos. En el evento, también se celebraron dos años de la llegada e

  9. Produção de bulbinhos de cebola em bandejas de isopor Production of onion bulb sets in styrofoam trays

    Directory of Open Access Journals (Sweden)

    Antonio Ismael Inácio Cardoso

    1999-10-01

    Full Text Available Com o objetivo de estudar a viabilidade de produção de bulbinhos de cebola sob ambiente protegido em diferentes tipos de bandejas de poliestireno expandido foram instalados dois experimentos. No primeiro, trabalhando com a cultivar Serrana, foram avaliadas três tipos de bandejas com diferentes números de plantas por célula: uma planta/célula na bandeja de 288 células, três a cinco plantas/célula na de 128 e cinco a nove plantas/célula na de 72 células. No outro experimento, testaram-se nove cultivares, semeadas somente em bandejas de 128 células com três plantas por célula. Utilizou-se o delineamento em blocos ao acaso com quatro repetições no primeiro ensaio e cinco no segundo, sendo cada parcela constituída por uma bandeja. Observou-se que quanto maior a densidade de plantio menor o tamanho do bulbinho (diâmetro e peso. O manejo adequado de adubações em cobertura pode viabilizar, tecnicamente, a produção de cinco bulbinhos com 1,3 cm de diâmetro (2 g por célula, na bandeja de 128 células. Quanto ao ensaio de cultivares, ‘Crioula’ da Agroflora, ‘Crioula’ da Granja Lotário e ‘Houston’ apresentaram 83%, 67% e 81% de plantas bulbificadas, enquanto que nas demais (‘Aurora’, ‘Baia Periforme’, ‘Brownsville’, ‘Petroline’, ‘Pira Ouro’, ‘Pirana’ e ‘Serrana’ obteve-se 100% de bulbificação. As principais vantagens na produção de bulbinhos em bandeja foram: uniformidade, ausência de plantas daninhas e de patógenos. É um sistema que pode ser útil aos viveiristas que tem entressafra de mudas nos meses de agosto a novembro, período de produção dos bulbinhos. Poderá, também, ser uma alternativa na produção de bulbinhos para conserva, no controle de qualidade de lotes de sementes e em programas de melhoramento de cebola.One objective was to study the production of onion Allium cepa L.. bulb sets in a protected environment in a seedling system with trays with different number of

  10. Evaluating Predators and Competitors in Wisconsin Red Pine Forests for Attraction to Mountain Pine Beetle Pheromones for Anticipatory Biological Control.

    Science.gov (United States)

    Pfammatter, Jesse A; Krause, Adam; Raffa, Kenneth F

    2015-08-01

    Mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), is an irruptive tree-killing species native to pine forests of western North America. Two potential pathways of spread to eastern forests have recently been identified. First, warming temperatures have driven range expansion from British Columbia into Albertan jack pine forests that are contiguous with the Great Lakes region. Second, high temperatures and drought have fostered largescale outbreaks within the historical range, creating economic incentives to salvage killed timber by transporting logs to midwestern markets, which risks accidental introduction. We evaluated the extent to which local predators and competitors that exploit bark beetle semiochemicals would respond to D. ponderosae in Wisconsin. We emulated D. ponderosae attack by deploying lures containing synthetic aggregation pheromones with and without host tree compounds and blank control traps in six red pine plantations over 2 yr. Predator populations were high in these stands, as evidenced by catches in positive control traps, baited with pheromones of local bark beetles and were deployed distant from behavioral choice plots. Only one predator, Thanasimus dubius F. (Coleoptera: Cleridae) was attracted to D. ponderosae's aggregation pheromones relative to blank controls, and its attraction was relatively weak. The most common bark beetles attracted to these pheromones were lower stem and root colonizers, which likely would facilitate rather than compete with D. ponderosae. There was some, but weak, attraction of potentially competing Ips species. Other factors that might influence natural enemy impacts on D. ponderosae in midwestern forests, such as phenological synchrony and exploitation of male-produced pheromones, are discussed.

  11. Effect of aerosol surface lubricants on the abundance and richness of selected forest insects captured in multiple-funnel and panel traps.

    Science.gov (United States)

    Allison, Jeremy D; Johnson, C Wood; Meeker, James R; Strom, Brian L; Butler, Sarah M

    2011-08-01

    Survey and detection programs for native and exotic forest insects frequently rely on traps baited with odorants, which mediate the orientation of target taxa (e.g., the southern pine beetle, Dendroctonusfrontalis Zimmermann) toward a resource (e.g., host material, mates). The influence of trap design on the capture efficiency of baited traps has received far less empirical attention than odorants, despite concerns that intercept traps currently used operationally have poor capture efficiencies for some target taxa (e.g., large woodborers). Several studies have recently demonstrated that treating traps with a surface lubricant to make them "slippery" can increase their capture efficiency; however, previously tested products can be expensive and their application time-consuming. The purpose of this study was to evaluate the effect of alternate, easier to apply aerosol lubricants on trap capture efficiency of selected forest insects. Aerosol formulations of Teflon and silicone lubricants increased both panel and multiple-funnel trap capture efficiencies. Multiple-funnel traps treated with either aerosol lubricant captured significantly more Monochamus spp. and Acanthocinus obsoletus (Olivier) than untreated traps. Similarly, treated panel traps captured significantly more Xylotrechus sagittatus (Germar), Ips calligraphus (Germar), Pissodes nemorensis (Germar), Monochamus spp., A. obsoletus, Thanasimus dubius (F.), and Ibalia leucospoides (Hochenwarth) than untreated traps. This study demonstrates that treating multiple-funnel and panel traps with an aerosol dry film lubricant can increase their capture efficiencies for large woodborers (e.g., Cerambycidae) as well as bark beetles, a weevil, a woodwasp parasitoid and a bark beetle natural enemy (Coleoptera: Cleridae).

  12. Phylogenetic relationships within the Callicebus cupreus species group (Pitheciidae: Primates): Biogeographic and taxonomic implications.

    Science.gov (United States)

    Hoyos, Manuel; Bloor, Paul; Defler, Thomas; Vermeer, Jan; Röhe, Fabio; Farias, Izeni

    2016-09-01

    The genus Callicebus (Thomas, 1903) is one of the most diverse of Neotropical primate genera and the only extant member of the Callicebinae subfamily. It has a widespread distribution from Colombia to Brazil, Bolivia, Peru and northern Paraguay. Coat colouring and colour pattern vary substantially within the genus, and this has led to the description of numerous species and subspecies, as well as numerous species groups. However, a lack of molecular phylogenetic analyses on the genus means that phylogenetic relationships and biogeographic history of species are poorly understood. Here, we examined phylogenetic relationships and patterns of diversification within the Callicebus cupreus species Group (sensu Kobayashi, 1995) using complete mitochondrial DNA cytochrome b gene sequence. Analyses indicate that the Callicebus cupreus Group underwent recent and extensive diversification. The common ancestor appears to have emerged some 2.3 million years ago (Ma) from a centre of origin in the western Amazon region, followed by diversification of the group between about 1.5 and 1.2Ma. Phylogenetic analyses were able to recover most previously described species (including the recently described Colombian endemic Callicebus caquetensis). However, there are some notable inconsistences between the obtained phylogeny and current taxonomy. Some previously recognized taxa were not separated by our data (e.g., Callicebus caligatus and Callicebus dubius), while currently unrecognized species diversity was uncovered within C. cupreus in the form of two divergent lineages: one of which exhibited greater phylogenetic similarity to species from the C. moloch Group. Based on the present study, we challenge current taxonomic arrangements for the C. cupreus species Group and call for a thorough taxonomic revision within the genus Callicebus.

  13. МОРФОФУНКЦИОНАЛЬНЫЕ ОСОБЕННОСТИ ОБОНЯТЕЛЬНЫХ ЖЕЛЕЗ МОРСКИХ РЫБ КАК ПРИМЕР КОНВЕРГЕНТНОЙ ТКАНЕВОЙ ЭВОЛЮЦИИ

    OpenAIRE

    ДОРОШЕНКО МАЙЯ АНДРЕЕВНА

    2014-01-01

    Гистологическими, гистохимическими и электронномикроскопическими методами изучались органы обоняния у морских рыб представителей отряда скорпенообразных (Scorpaeniformes). У Pleurogrammus azonus, Myoxocephalus yaok, Enophrys diceraus, Hemilepidotus gilberti, Hemitripterus villosus, Podothecus veternus и Liparis dubius обнаружены обонятельные железы. Обонятельные железы также отмечены в органах обоняния рыб отряда сельдеобразных (Clupeiformes), лососеобразных (Salmoniformes) и иглобрюхообразны...

  14. Belowground eco-restoration of a suburban waste-storage landscape: Earthworm dynamics in grassland and in a succession of woody vegetation covers☆

    Science.gov (United States)

    Morales, Pamela K.; Yunusa, Isa A.M.; Lugg, Glenys; Li, Zheng; Gribben, Paul; Eamus, Derek

    2013-01-01

    Restoration of belowground ecology is seldom a priority in designing revegetation strategies for disturbed landscapes. We determined earthworm abundance and diversity in a 16-year old grass sward (grassland), a 6-year old (Plantation-04) and a 4-year old (Plantation-06) plantation, both of mixed woody species, on a reclaimed waste disposal site, and in nearby remnant woodland, in suburban Sydney, Australia. While no catches were made in autumn, more earthworms were found in spring (21 ± 8.6 m–2) than in winter (10.2 ± 5.9 m–2) or summer (14.4 ± 5.5 m–2). Earthworm abundance in spring was in the order grassland ≈ Plantation-04 (35.2 m–2) > woodland (12.8 m–2) > Plantation-06 (0.8 m–2). None of the revegetated covers had restored earthworm diversity to levels found in the woodland. Exotic species, mostly Microscolex dubius, dominated in the four vegetation covers at any time; the only two native species (Heteroporodrilus sp. and Megascoleceides sp.) found were in the woodland. We also assessed how quality of the evolving soils from the three revegetated covers, compared with that from the woodland, impacted viability of common exotic earthworm species. Both weight gain and cocoon production by the exotic earthworms were higher in the soil from Plantation-04 than in soils from the other vegetation covers, including the woodland; the two variables were positively correlated with the pH and mineral nutrient content (as indicated by electrical conductivity that was in turn correlated with clay content) of the soil. Age of vegetation rather than its composition explained differences in the level of earthworm recovery observed. PMID:25550677

  15. Belowground eco-restoration of a suburban waste-storage landscape: Earthworm dynamics in grassland and in a succession of woody vegetation covers.

    Science.gov (United States)

    Morales, Pamela K; Yunusa, Isa A M; Lugg, Glenys; Li, Zheng; Gribben, Paul; Eamus, Derek

    2013-12-01

    Restoration of belowground ecology is seldom a priority in designing revegetation strategies for disturbed landscapes. We determined earthworm abundance and diversity in a 16-year old grass sward (grassland), a 6-year old (Plantation-04) and a 4-year old (Plantation-06) plantation, both of mixed woody species, on a reclaimed waste disposal site, and in nearby remnant woodland, in suburban Sydney, Australia. While no catches were made in autumn, more earthworms were found in spring (21 ± 8.6 m(-2)) than in winter (10.2 ± 5.9 m(-2)) or summer (14.4 ± 5.5 m(-2)). Earthworm abundance in spring was in the order grassland ≈ Plantation-04 (35.2 m(-2)) > woodland (12.8 m(-2)) > Plantation-06 (0.8 m(-2)). None of the revegetated covers had restored earthworm diversity to levels found in the woodland. Exotic species, mostly Microscolex dubius, dominated in the four vegetation covers at any time; the only two native species (Heteroporodrilus sp. and Megascoleceides sp.) found were in the woodland. We also assessed how quality of the evolving soils from the three revegetated covers, compared with that from the woodland, impacted viability of common exotic earthworm species. Both weight gain and cocoon production by the exotic earthworms were higher in the soil from Plantation-04 than in soils from the other vegetation covers, including the woodland; the two variables were positively correlated with the pH and mineral nutrient content (as indicated by electrical conductivity that was in turn correlated with clay content) of the soil. Age of vegetation rather than its composition explained differences in the level of earthworm recovery observed.

  16. VICHADA: LA HOSPITALIDAD DEL ORINOCO. Pag. 150-157

    Directory of Open Access Journals (Sweden)

    Wilmer Velandia

    2013-03-01

    Full Text Available Donde el cielo y la sabana se unen en el infinito, se vislumbra una sola montaña en la lejanía, parece un gigante, antiguo y oscuro al final de la inmensidad un “Tepuye”, después de unas cuantas horas de camino se acerca este gran anciano que data del precámbrico, pero no está solo. Se observan algunos compañeros iguales a él, se podrían considerar como los guardianes del imponente y hermoso Orinoko, considerado en lengua Sikuani como la gran serpiente enroscada acompañada de una gran riqueza paisajística cultural y biodiversa; bosques de galería, diferentes tipos de sabana, esteros, lagunas, morichales, bosques inundables, las que recorren aguas blancas, negras y mixtas, rodeadas por diferentes etnias y grupos indígenas como Piaroas, Sikuanis – Guahibos, Cuibas y Amoruas, que viven en forma pacífica con llaneros y colonos, orgullosos de estas tierras, amables y hospitalarios que desde hace muchos años comparten este paraíso con: bagres, pirañas, anguilas, rayas, arawanas, anacondas, ranas, sapos, caimanes, babos, iguanas, tortugas, loros, búhos, guacamayas, azucareros, pavones, paujiles, águilas, zamuros, aulladores, armadillos, ocarros, chigüiros, osos hormigueros, ocelotes, jaguares, nutrias, murciélagos, cuerpo espines, zorros, venados y delfines, que pasan sus días entre; cedros, moriches, saladillos, caraños, yarumos, laureles, caimos, guaduas, guamos, alcornoques, chaparros, arepillos, palmiches y gualtes.

  17. Eph/ephrin-B-mediated cell-to-cell interactions govern MTS20(+) thymic epithelial cell development.

    Science.gov (United States)

    Montero-Herradón, Sara; García-Ceca, Javier; Sánchez Del Collado, Beatriz; Alfaro, David; Zapata, Agustín G

    2016-08-01

    Thymus development is a complex process in which cell-to-cell interactions between thymocytes and thymic epithelial cells (TECs) are essential to allow a proper maturation of both thymic cell components. Although signals that control thymocyte development are well known, mechanisms governing TEC maturation are poorly understood, especially those that regulate the maturation of immature TEC populations during early fetal thymus development. In this study, we show that EphB2-deficient, EphB2LacZ and EphB3-deficient fetal thymuses present a lower number of cells and delayed maturation of DN cell subsets compared to WT values. Moreover, deficits in the production of chemokines, known to be involved in the lymphoid seeding into the thymus, contribute in decreased proportions of intrathymic T cell progenitors (PIRA/B(+)) in the mutant thymuses from early stages of development. These features correlate with increased proportions of MTS20(+) cells but fewer MTS20(-) cells from E13.5 onward in the deficient thymuses, suggesting a delayed development of the first epithelial cells. In addition, in vitro the lack of thymocytes or the blockade of Eph/ephrin-B-mediated cell-to-cell interactions between either thymocytes-TECs or TECs-TECs in E13.5 fetal thymic lobes coursed with increased proportions of MTS20(+) TECs. This confirms, for the first time, that the presence of CD45(+) cells, corresponding at these stages to DN1 and DN2 cells, and Eph/ephrin-B-mediated heterotypic or homotypic cell interactions between thymocytes and TECs, or between TECs and themselves, contribute to the early maturation of MTS20(+) TECs.

  18. Accuracy of a self-collection kit for the microbiological study of the vaginal content

    Directory of Open Access Journals (Sweden)

    Mauro Romero L. Passos

    Full Text Available Diagnosis of vaginal discharge is frequently performed in an empirical way, leading to inadequate treatment. This study tested the accuracy of a self-collection kit for microbiological study of the vaginal content. One hundred and forty-two women of Family Health Program units in Niterói and Piraí cities were enrolled in order to have their vaginal content studied. A brief explanation and a self-collection kit were provided in order to sample the vaginal content. The self-collection kit was composed of one empty plastic tube, two glass slides, a long handle cytobrush, an identification card and guideline notes. The vaginal sample was applied on the glass slides by the women and stained by Gram technique. A second sampling was done by the medical personnel. The microbiological diagnosis in a blinded analysis was made under optical microscopy. A validation diagnosis test was done taking the medical collection results as a gold standard. A total of 106 women had followed the protocol and were included in the study. Microbiological analysis was unsatisfactory in 12 cases (6 cases of self-collection material and 6 cases of medical collection. The microbiological analyses in the self-collection and in the medical collection material were respectively: bacterial vaginosis in 21.7% and 17.9%, non bacillar flora in 10.3% and 11.3%, vaginal trichomoniasis in 5.66% and 5.6%, candidiasis in 3.78% and 2.8% and a normal microbiota in 52.8% and 56.6%. The Kappa coefficient suggested a "very good correlation" of the microbiological results between the two methods of collection (K=0.7945. The self-collection kit provides samples for microbiological analysis of the vaginal microbiota as good as medical collection.

  19. ECOLOGICAL FACTORS ASSOCIATED WITH THE ESTABLISHMENT OF RUSTIC SPECIES IN DISTURBED ECOSYSTEMS IN THE ATLANTIC FOREST, PIRAÍ, RIO DE JANEIRO STATE – BRAZIL

    Directory of Open Access Journals (Sweden)

    Hiram Feijó Baylão Junior

    2013-08-01

    Full Text Available http://dx.doi.org/10.5902/1980509810542 Disturbed ecosystems do not present original floristic composition. Their soils are depleted, shallow, stony, with low infiltration and present erosion with different levels of Geodynamics. The region has areas of pasture with sparse herbaceous vegetation which is weakened in every rain and fire, covering less ground. The individuals that colonized and settled in these environments were considered rustic species. This study raised and identified by census, the rustic species and their structure in the most degraded part (lower third of the watershed of Cacaria’s river at the base of the Serra do Mar, Piraí, Rio de Janeiro state, and evaluated the influence of ecological exposure, slope, elevation, topography and rock outcrops in the establishment and growth of these species. For the vegetation survey it was conducted census in an area of 22 hectares, where it was measured, geo-referenced and identified all spontaneous tree species that were isolated in a pasture area. Ecological factors exposure, elevation and slope were determined with a compass, altimeter and clinometer, respectively. We identified 131 individuals, representing 14 species, grouped into nine families. Tabernaemontana laeta Mart., Sparattosperma leucanthum (Vell. Schum., Machaerium hirtum (Vell. Stellfeld, Tabebuia chrysotricha (Mart. ex DC. Stan., Cecropia pachystachya Trec., Peltophorum dubium (Spreng. Taub., Guarea guidonia (L. Sleumer, Acacia polyphylla DC. and Psidium guajava L.were present in portions of the slope with exposure to the north, with altitudes from 60m to 80m and with slope strongly corrugated (20-45%, indicating a preference of these species for microhabitats with those characteristics. 

  20. Accuracy of a self-collection kit for the microbiological study of the vaginal content

    Directory of Open Access Journals (Sweden)

    Mauro Romero L. Passos

    2007-04-01

    Full Text Available Diagnosis of vaginal discharge is frequently performed in an empirical way, leading to inadequate treatment. This study tested the accuracy of a self-collection kit for microbiological study of the vaginal content. One hundred and forty-two women of Family Health Program units in Niterói and Piraí cities were enrolled in order to have their vaginal content studied. A brief explanation and a self-collection kit were provided in order to sample the vaginal content. The self-collection kit was composed of one empty plastic tube, two glass slides, a long handle cytobrush, an identification card and guideline notes. The vaginal sample was applied on the glass slides by the women and stained by Gram technique. A second sampling was done by the medical personnel. The microbiological diagnosis in a blinded analysis was made under optical microscopy. A validation diagnosis test was done taking the medical collection results as a gold standard. A total of 106 women had followed the protocol and were included in the study. Microbiological analysis was unsatisfactory in 12 cases (6 cases of self-collection material and 6 cases of medical collection. The microbiological analyses in the self-collection and in the medical collection material were respectively: bacterial vaginosis in 21.7% and 17.9%, non bacillar flora in 10.3% and 11.3%, vaginal trichomoniasis in 5.66% and 5.6%, candidiasis in 3.78% and 2.8% and a normal microbiota in 52.8% and 56.6%. The Kappa coefficient suggested a "very good correlation" of the microbiological results between the two methods of collection (K=0.7945. The self-collection kit provides samples for microbiological analysis of the vaginal microbiota as good as medical collection.

  1. Selected Abstracts of the 11th International Workshop on Neonatology; Cagliari (Italy; October 26-31, 2015

    Directory of Open Access Journals (Sweden)

    --- Various Authors

    2015-10-01

    . Piras, A. Ravarino, R. Ambu • Cagliari and Pisa (Italy ABS 18. Pulmonary atresia: the importance of prenatal diagnosis • A.L. Costa, H. Soares, A.L. Neves, C. Moura, J.C. Areias, A.C. Fragoso, J. Estevão-Costa, H. Guimarães • Porto (Portugal ABS 19. The use of hypothermia in preterm asphyxiated neonate: new results from a metabolomics point of view • E. d’Aloja, A. Noto, E. Locci, C. Dalmazzo, G. Pomero, A. Dessì, V. Fanos, P. Gancia • Cagliari and Cuneo (Italy ABS 20. Alopecia areata in a child: case report, management and therapy • F. Farnetani, V.D. Mandel, G. Pellacani • Modena (Italy ABS 21. Physiological and therapeutic roles of mesenchymal stem cells. Is there a gap? • U. Galderisi, F. Pentimalli, A. Giordano • Philadelphia (PA, USA, Naples and Siena (Italy, Kayseri (Turkey ABS 22. Esophageal impedance baseline is correlated with exposure to reflux in newborns with GERD symptoms • F. Cresi, E. Locatelli, E. Maggiora, A. Pirra, C. Peila, E. Bertino • Turin (Italy ABS 23. Analytical pitfalls and future perspectives in the measurement of bone metabolism biomarkers • A. Fortunato • Vicenza (Italy ABS 24. Genetic analysis of surfactant proteins genes in premature newborn infants with severe respiratory distress syndrome • M. Somaschini, S. Presi, M. Ferrari P. Carrera • Milan (Italy ABS 25. Child abuse and neglect: an increasing childhood’s condition • S. Cherubini • Busto Arsizio (Italy ABS 26. When a newborn dies: European approaches to responsibility • F. Tregnaghi, R. Carter, J. Ferguson, K. Nijs • Verona (Italy, Devon (UK, and Leuven (Belgium ABS 27. NMR-based screening possibilities in the neonate and comparison to mass spectral methods • M. Spraul, C. Cannet, H. Schäfer • Rheinstetten (Germany ABS 28. Our team project: learning from mistakes • M. Susi, E.M. Padovani, A. Bertolini, M. Lavarini, S. Tardivo • Verona (Italy ABS 29. Peripheral intravenous catheters in neonates • M. Zicchi, A. Farbo • Sassari

  2. A systematic revision of Operclipygus Marseul (Coleoptera, Histeridae, Exosternini

    Directory of Open Access Journals (Sweden)

    Michael Caterino

    2013-02-01

    . panamensis (Wenzel & Dybas, 1941], O. sejunctus group [O. depressus (Hinton, 1935, O. itoupe sp. n., O. juninensis sp. n., O. pecki sp. n., O. punctiventer sp. n., O. sejunctus (Schmidt, 1896 comb. n., O. setiventris sp. n.], O. mortavis group [O. ecitonis sp. n., O. mortavis sp. n., O. paraguensis sp. n.], O. dytiscoides group [O. carinisternus sp. n., O. crenulatus sp. n., O. dytiscoides sp. n., O. quadratus sp. n.], O. dubitabilis group [O. dubitabilis (Marseul, 1889, O. yasuni sp. n.], O. angulifer group [O. angulifer sp. n., O. impressifrons sp. n.], O. dubius group [O. andinus sp. n., O. dubius (Lewis, 1888, O. extraneus sp. n., O. intermissus sp. n., O. lunulus sp. n., O. occultus sp. n., O. perplexus sp. n., O. remotus sp. n., O. validus sp. n., O. variabilis sp. n.], O. hospes group [O. assimilis sp. n., O. belemensis sp. n., O. bulbistoma sp. n., O. callifrons sp. n., O. colombicus sp. n., O. communis sp. n., O. confertus sp. n., O. confluens sp. n., O. curtistrius sp. n., O. diffluens sp. n., O. fusistrius sp. n., O. gratus sp. n., O. hospes (Lewis, 1902, O. ibiscus sp. n., O. ignifer sp. n., O. impositus sp. n., O. incisus sp. n., O. innocuus sp. n., O. inquilinus sp. n., O. minutus sp. n., O. novateutoniae sp. n., O. praecinctus sp. n., O. prominens sp. n., O. rileyi sp. n., O. subterraneus sp. n., O. tenuis sp. n., O. tiputinus sp. n.], O. farctus group [O. atlanticus sp. n., O. bidessois (Marseul, 1889, O. distinctus (Hinton, 1935, O. distractus (Schmidt, 1896 comb. n., O. farctissimus sp. n., O. farctus (Marseul, 1864, O. gilli sp. n., O. impressistrius sp. n., O. inflatus sp. n., O. latemarginatus (Bickhardt, 1920 comb. n., O. petrovi sp. n., O. plicatus (Hinton, 1935 comb. n., O. prolixus sp. n., O. punctifrons sp. n., O. proximus sp. n., O. subrufus sp. n.], O. hirsutipes group [O. guianensis sp. n., O. hirsutipes sp. n.], O. hamistrius group [O. arquus sp. n., O. campbelli sp. n., O. chiapensis sp. n., O. dybasi sp. n., O. geometricus (Casey, 1893 comb. n

  3. 北京植物区系新资料%SOME NEWLY RECORDED FLORA PLANTS FROM BEIJING

    Institute of Scientific and Technical Information of China (English)

    刘全儒; 张劲林

    2014-01-01

    Twelve (12)species are reported as new flora in Beijing in this paper.These species include Saururus chinensis (Lour.)Baill.,Cocculus orbiculatus (Linn.)DC.,Chenopodium ambrosioides L.,Ch. pumilio R.Br.,Mirabilis nyctaginea (Michx.)MacMill.,Cabomba caroliniana A.Gray,Solanum lyratum Thunb.,S.sarrachoides Sendtner,Physalis pubescens L.,Carpesium abrotanoides L.,Tragopogon dubius Scop.,Mariscus umbellatus Vahl.Thalictrum foeniculaceum Bunge and Tagetes minuta L.are reported as supplementary distribution in Beijing area. Most of these species are naturalized except Thalictrum foeniculaceum Bunge.Saururus,Cocculus,Cabomba,Tragopogon and Mariscus are newly recorded genera, and Saururaceae is newly recorded family of Beij ing flora.%报道了北京植物区系新记录共12种,它们是三白草(Saururus chinensis (Lour.)Baill.)、木防己(Cocculus orbiculatus (L.)DC.)、土荆芥(Chenopodium ambrosioides L.)、铺地藜(Chpumilio R.Br.)、夜香紫茉莉(Mirabilis nyctaginea (Michx.)MacMill.)、水盾草(Cabomba caroliniana A.Gray)、白英(Solanum lyratum Thunb.)、腺龙葵(S sarrachiodes Sendtner.)、毛酸浆(Physalis pubescens L.)、天名精(Carpesium abrotanoides L.)、长喙婆罗门参(Tragopogondubius Scop.)、砖子苗(Mariscusumbellatus Vahl);此外,还补充了丝叶唐松草(Thalictrumfoeniculaceum Bunge)和小花万寿菊(Tagetes minuta L.)在北京的新分布地点.除丝叶唐松草外,其余种均为归化物种.三白草科(Saururaceae)为北京新记录科,而三白草属(Saururus),木防已属(Cocculus),水盾草属(Cabomba),婆罗门参属(Tragopogon)和转子苗属(Mariscus)为北京新记录属.

  4. The flat bark beetles (Coleoptera, Silvanidae, Cucujidae, Laemophloeidae of Atlantic Canada

    Directory of Open Access Journals (Sweden)

    Christopher Majka

    2008-09-01

    Full Text Available As a result of the present investigations 18 species of flat bark beetles are known to occur in Atlantic Canada, 10 in New Brunswick, 17 in Nova Scotia, four on Prince Edward Island, six on insular Newfoundland, and one in Labrador. Twenty-three new provincial records are reported and nine species, Uleiota debilis (LeConte, Uleiota dubius (Fabricius, Nausibius clavicornis (Kugelann, Ahasverus advena (Waltl, Cryptolestes pusillus (Schönherr, Cryptolestes turcicus (Grouvelle, Charaphloeus convexulus (LeConte, Charaphloeus species nr. adustus, and Placonotus zimmermanni (LeConte are newly recorded in the region, one of which C. sp. nr. adustus, is newly recorded in Canada as a whole. Eight species are cosmopolitan species introduced to the region and North America, nine are native Nearctic species, and one, Pediacus fuscus Erichson, is Holarctic in distribution. All the introduced species except for one (Silvanus bidentatus (Fabricius, a saproxylic species are found on various stored products, whereas all the native species are saproxylic. Ahasverus longulus (Blatchley, is removed from the species list of New Brunswick and Charophloeus adustus (LeConte is removed from the species list of Nova Scotia. One tropical Asian species, Cryptamorpha desjardinsi (Guérin-Méneville, has been intercepted in the region in imported produce, but is not established. The substantial proportion (44% of the fauna that is comprised of introduced species is highlighted, almost all of which are synanthropic species associated with various dried stored products. The island faunas of Prince Edward Island, Cape Breton Island, and insular Newfoundland are diminished in comparison to the mainland fauna, that of Prince Edward Island being exceptionally so in comparison to other saproxylic groups found there. Of the ten native species, four can be categorized as 'apparently rare' (i.e., comprising ≤ 0.005% of specimens examined from the region. It is possibly that the

  5. Revision of Khawia spp. (Cestoda: Caryophyllidea), parasites of cyprinid fish, including a key to their identification and molecular phylogeny.

    Science.gov (United States)

    Scholz, Tomás; Brabec, Jan; Král'ová-Hromadová, Ivica; Oros, Mikulás; Bazsalovicsová, Eva; Ermolenko, Alexey; Hanzelová, Vladimíra

    2011-09-01

    Monozoic cestodes of the genus Khawia Hsü, 1935 (Caryophyllidea: Lytocestidae), parasites of cyprinid fish in Europe, Asia, Africa and North America, are revised on the basis of taxonomic evaluation of extensive materials, including recently collected specimens of most species. This evaluation has made it possible to critically assess the validity of all 17 nominal species of the genus and to provide redescriptions of the following seven species considered to be valid: Khawia sinensis Hsü, 1935 (type species); K. armeniaca (Cholodkovsky, 1915); K. baltica Szidat, 1941; K. japonensis (Yamaguti, 1934); K. parva (Zmeev, 1936); K. rossittensis (Szidat, 1937); and K. saurogobii Xi, Oros, Wang, Wu, Gao et Nie, 2009. Several new synonyms are proposed: Khawia barbi Rahemo et Mohammad, 2002 and K. lutei Al-Kalak et Rahemo, 2003 are synonymized with K. armeniaca; K. coregoni Kritscher, 1990 with Caryophyllaeus laticeps (Pallas, 1781) (family Caryophyllaeidae); K. cyprini Li, 1964 and K. iowensis Calentine et Ulmer, 1961 with K. japonensis; K. dubia (Szidat, 1937) (syn. Bothrioscolex dubius Szidat, 1937) with K. rossittensis; and Tsengia neimongkuensis Li, 1964 and T. xiamenensis Liu, Yang et Lin, 1995 with K. sinensis. Khawia prussica (Szidat, 1937) (syn. Bothrioscolex prussicus Szidat, 1937) is considered to be species incertae sedis, but its morphology indicates it may belong to Caryophyllaeus Gmelin, 1790 (Caryophyllaeidae). The molecular analysis of all seven valid species, based on comparison of sequences of two nuclear ribosomal and two mitochondrial genes, has shown that the species form three major groups clustered according to their fish hosts. Five species from common and crucian carp and goldfish were grouped together, whereas K. armeniaca from barbels (Barbinae) and K. baltica from tench (Tinca) formed separate clades. In contrast, geographical distribution does not seem to play a crucial role in grouping of individual taxa. A phylogenetic tree based on

  6. Características estruturais de ácidos húmicos isolados de solos com adição de resíduos orgânicos de origem urbana: composição elementar, análise de RMN 13C e Pi-CG/EM

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    L. P. Canellas

    2002-06-01

    Full Text Available Foram avaliadas as alterações nas características estruturais de ácidos húmicos (AH decorrentes da adição de matéria orgânica de resíduos urbanos. Os AH foram extraídos da camada superficial (0-20 cm de um Argissolo Vermelho-Amarelo (Seropédica, RJ e de um Latossolo Vermelho Amarelo (Piraí, RJ, ambos tratados com o equivalente a 80 t ha-1 (em base seca de composto de lixo urbano e lodo de estação de tratamento de esgotos. O tempo de incubação em laboratório foi de 24 semanas à temperatura ambiente e umidade mantida na capacidade de campo. Para caracterizar os AH, foram utilizadas a análise elementar, a ressonância magnética nuclear (RMN de 13C e Pirólise acoplada à cromatografia gasosa e a espectrometria de massas (Pi-CG/EM. A análise de RMN 13C permitiu observar alterações na composição molecular dos AH de forma clara, principalmente, pelo aumento do teor de C mono e di oxigenados (δC 50-110 e pela diminuição dos teores de grupos CH2 e CH3 (δC 50-110. O aumento no teor de carboidratos evidencia a presença de estruturas mais lábeis nos AH com adição dos resíduos. Em adição, a técnica de Pi-CG/EM permitiu verificar diferenças qualitativas significativas nos compostos orgânicos, provenientes da fragmentação térmica dos AH com incorporação de novas estruturas, sobretudo da fração lipídica e de derivados de carboidratos (furanos, nas amostras de solos tratados com ambos os resíduos orgânicos. O uso em conjunto das técnicas analíticas de RMN 13C e de Pi-CG/EM foi eficiente para avaliar as mudanças na composição molecular de ácidos húmicos decorrentes da adição de resíduos orgânicos de origem urbana.

  7. Dot-ELISA-IgM in saliva for the diagnosis of human leptospirosis using polyester fabric-resin as support (Preliminary Report Dot-ELISA-IgM em saliva para diagnóstico da leptospirose humana, empregando como suporte tecido de poliéster-resina (Nota Prévia

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    Marcos Vinicius da Silva

    1994-10-01

    Full Text Available In order to improve the diagnosis of human leptospirosis, we standardized the dot-ELISA for the search of specific IgM antibodies in saliva. Saliva and serum samples were collected simultaneously from 20 patients with the icterohemorrhagic form of the disease, from 10 patients with other pathologies and from 5 negative controls. Leptospires of serovars icterohaemorrhagiae, canicola, hebdomadis, brasiliensis and cynopteri grown in EMJH medium and mixed together in equal volumes, were used as antigen at individual protein concentration of 0.2 µg/µl. In the solid phase of the test we used polyester fabric impregnated with N-methylolacrylamide resin. The antigen volume for each test was 1µl, the saliva volume was 8 µl, and the volume of peroxidase-labelled anti-human IgM conjugate was 30 µl. A visual reading was taken after development in freshly prepared chromogen solution. In contrast to the classic nitrocellulose membrane support, the fabric support is easy to obtain and to handle. Saliva can be collected directly onto the support, a fact that facilitates the method and reduces the expenses and risks related to blood processing.Com a finalidade de melhorar o diagnóstico da leptospirose humana, padronizou-se o teste dot-ELISA para a pesquisa de anticorpos específicos da classe IgM na saliva. Empregaram-se amostras de saliva e soro coletadas simultaneamente de 20 pacientes com a forma ictero-hemorrágica da doença, de 10 pacientes com outras patologias e 5 controles negativos. Culturas de Leptos-pira em meio EMJH, dos sorovares: icterohaemorrhagiae, canicola, hebdomadis, brasiliensis e cynopteri, foram utilizadas como antígeno, na concentração proteica individual de 0,2 µg/µl, misturadas em volumes iguais. Na fase sólida do teste empregou-se tecido de po-liéster impregnado com resina de N-metilol-acrilamida. O volume do antígeno para cada teste foi de 1µl, o de saliva 8µl, o de conjugado anti-IgM humana marcada com peroxidase, de

  8. Sinopsis del mundo Piro: Ética y estética en el arte Piro. Modificaciones en las estructuras de parentesco Piro durante el auge del caucho (1885-1915. Los asentamientos Piros y la persistencia de su identidad

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    Luis Román Villanueva

    1985-12-01

    Full Text Available Los tres artículos que a continuación presentamos tienen como objeto detallar tres aspectos fundamentales de la cultura Piro. Este grupo etno-linguistico, pertenece a la familia Arahuaca, al igual que los Campa, Machiguenga y Amuesha, se ubica principalmente en las riberas del río Urubamba perteneciente al distrito de Echarate, Provincia de la Convención, Departamento del Cusco y el Distrito de Sepahua, Provincia de Atalaya, Departamento de Ucayali. La población estimada para este grupo es de 1600 habitantes (Wise, M.R. 1972 que se distribuye en 12 comunidades nativas, de las cuales la mitad están tituladas por el Estado peruano. Sobre este grupo existe regular bibliografía principalmente en lo referido a lingüística, relatos de viajeros y misioneros de diversas órdenes y en menor escala trabajos etnográficos y etno-históricos. En esta serie de artículos los autores pretenden una aproximación en detalle para profundizar el conocimiento que de esta etnia se tiene. Para ello utilizaremos los trabajos anteriores a los cuales sumaremos nuestras notas y apreciaciones recogidas durante cuatro años de trabajo en la zona. El primer artículo de L. Komán pretende explicar la relación entre lo ideológico y lo estético para lo cual se remite a la mitología y las manifestaciones estéticas del grupo piro. En el segundo artículo el mismo autor señala una transformación estructural en el sistema de parentesco piro durante el auge de la explotación del caucho, dando lugar a una variación del modelo original, que garantiza la reproducción biológica y cultural del grupo. El artículo final de V. de Szyzslo describe la forma cómo cada una de las familias de la comunidad pira de Miaría, en el Urubamba, dispone del espacio para la construcción de sus viviendas y locales comunales. Los tres artículos, por lo tanto son complementarios para obtener una visión en detalle de los aspectos ideológicos, socio-políticos y tecnológicos q u

  9. Identification of CVM and DUMPS Carriers in Chinese Holstein Bulls%中国荷斯坦公牛脊椎畸形综合征和尿苷酸合酶缺乏症遗传缺陷检测

    Institute of Scientific and Technical Information of China (English)

    梁若冰; 谢岩; 范学华; 张毅; 孙东晓; 张胜利

    2014-01-01

    本研究旨在研究中国荷斯坦公牛脊椎畸形综合征(Complex vertebral malformation,CVM)和尿苷酸合酶缺乏症(Deficiency of uridine monophosphate synthase,DUMPS)两种遗传缺陷的携带者及系谱来源.通过PIRA-PCR和PCR-RFLP方法对参加全国青年公牛联合后裔测定和国家良种补贴项目的、来自全国27个公牛站的691头荷斯坦公牛分别进行了CVM和DUMPS的遗传缺陷检测,共发现34头CVM和1头DUMPS隐性有害基因携带者公牛,携带者比例分别为4.92%和0.14%,隐性有害等位基因频率分别为2.46%和0.07%.经过系谱分析发现,其中28头CVM携带者均为美国公牛Carlin-M Ivanhoe Bell的后代,另外6头因系谱不完整而无法查询.1头DUMPS携带者为美国公牛Skokie Sensation Ned后代.因此,我国有必要尽快建立荷斯坦牛隐性遗传缺陷监控体系并进行系谱标注,通过青年公牛预选和选种选配,避免携带者公牛进入后裔测定和良种补贴项目,以逐步降低我国奶牛群体中隐性有害等位基因频率,为提高奶牛群体质量提供依据.

  10. Ocorrência de plantas aquáticas nos reservatórios da Light-RJ Ocurrence of aquatic plants in the Light-RJ reservoirs

    Directory of Open Access Journals (Sweden)

    D. Martins

    2003-01-01

    Full Text Available O objetivo do presente trabalho foi caracterizar as comunidades infestantes de plantas aquáticas presentes nos reservatórios da Light-Sistema de Eletricidade S.A., localizada no município de Piraí-RJ. Os levantamentos foram realizados no período de julho a setembro de 1998. Os reservatórios analisados foram: Vigário, Pereira Passos e Lajes, sendo as quantidades de pontos amostrados de 19, 9 e 15, respectivamente. Em cada ponto amostrado fez-se a marcação das coordenadas geográficas e avaliou-se a porcentagem de ocupação do corpo d'água pelas espécies de plantas aquáticas presentes. Depois da identificação das plantas, pôde-se verificar quais eram as espécies mais freqüentes e a sua distribuição dentro do sistema de geração de energia. As espécies encontradas nos reservatórios foram: Brachiaria arrecta (Hack. Stent.; Egeria densa Planch.; Eichhornia azurea (Sw. Kunth.; Eichhornia crassipes (Mart. Solms.; Hymenachne amplexicaulis (Rudge Nees.; Panicum rivulare Trin.; Pistia stratiotis L.; Polygonum spp.; Sagitaria montevidensis Cham. & Schlecht; Salvinia auriculata (Micheli Adans; e Thypha dominguensis L.This study aimed to describe the aquatic weeds infesting the Light-Electric System S.A. reservoirs, located in Pirai-RJ, Brazil. The survey was carried out from July to September 1998. The reservoirs analyzed were: Vigario, Pereira Passos and Lajes, with 19, 9 and 15 points being sampled, respectively. Sampled geographic coordinates were marked in each point and a visual estimate was made of the percentage of river water occupied by the aquatic plants. After plant identification, the most frequent species and their distribution were determined as follows: Brachiaria arrecta (Hack. Stent.; Egeria densa Planch.; Eichhornia azurea (Sw. Kunth.; Eichhornia crassipes (Mart. Solms.; Hymenachne amplexicaulis (Rudge Nees.; Panicum rivulare Trin.; Pistia stratiotis L.; Polygonum spp.; Sagitaria montevidensis Cham.& Schlecht

  11. Detection of sdhB gene mutations in SDHI-resistant isolates of Botrytis cinerea using high resolution melting (HRM analysis

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    Anastasios Samaras

    2016-11-01

    Full Text Available Botrytis cinerea, is a high-risk pathogen for fungicide resistance development. Pathogen` resistance to SDHIs is associated with several mutations in sdh gene. The diversity of mutations and their differential effect on cross-resistance patterns among SDHIs and the fitness of resistant strains necessitate the availability of a tool for their rapid identification. This study was initiated to develop and validate a high-resolution melting (HRM analysis for the identification of P225H/F/L//T, N230I and H272L/R/Y mutations. Based on the sequence of sdhB subunit of resistant and sensitive isolates, a universal primer pair was designed. The specificity of the HRM analysis primers was verified to ensure against the cross-reaction with other fungal species and its sensitivity was evaluated using concentrations of known amounts of mutant`s DNA. The melting curve analysis generated nine distinct curve profiles, enabling the discrimination of all the 4 mutations located at codon 225, the N230I mutation, the 3 mutations located in codon 272 and the non mutated isolates (isolates of wild type sensitivity. Similar results were obtained when DNA was extracted directly from artificially inoculated strawberry fruit. The method was validated by monitoring the presence of sdhB mutations in samples of naturally infected strawberry fruits and stone fruit rootstock seedling plants showing damping off symptoms. HRM analysis data were compared with a standard PIRA-PCR technique and an absolute agreement was observed suggesting that in both populations the H272R mutation was the predominant one, while H272Y, N230I and P225H were detected in lower frequencies. The results of the study suggest that HRM analysis can be a useful tool for sensate, accurate and rapid identification of several sdhB mutations in B. cinerea and it is expected to contribute in routine fungicide resistance monitoring or assessments of the effectiveness of antiresistance strategies implemented in

  12. Litoquímica, Geocronologia U-Pb e Geologia Isotópica (Sr-Nd-Pb das Rochas Graníticas dos Batólitos Cunhaporanga e Três Córregos na Porção Sul do Cinturão Ribeira, Estado do Paraná

    Directory of Open Access Journals (Sweden)

    Hélcio José dos Prazeres Filho

    2003-08-01

    Full Text Available Durante a evolução da porção sul do Cinturão Ribeira no Estado do Paraná, o Ciclo Brasiliano-Pan Africano foi responsávelpela geração de um grande volume de rochas graníticas com destaque para os batólitos Cunhaporanga (BCP e TrêsCórregos (BTC. Estudos litoquímicos e isotópicos revelaram a existência de tipos graníticos distintos entre dois batólitos einternamente aos mesmos, definidos por unidades graníticas. A principal diferença entre os dois batólitos está no comportamentodos álcalis com o BCP, a NW mais potássico e o BTC, a SE mais sódico. Os dados isotópicos mostram claramente aparticipação de fontes infracrustais distintas de longa residência crustal, evidenciadas pelos isótopos de Pb em K-feldspato,pelos valores negativos de eNd (–11 a –13 para o BCP e –17 a –19 para o BTC e pelas idades TDM (1,8 a 2,0 Ga para o BCPe 2,2 a 2,4 Ga para o BTC. Estas fontes estariam situadas em uma crosta continental paleoproterozóica com a participaçãode fontes mantélicas e supracrustais na gênese das rochas graníticas do BCP e BTC. A Unidade Ribeirão Butiá do BCP e asUnidades Arrieiros e Paina do BTC estão representadas predominantemente por monzogranitos e granodioritos e rarostonalitos, metaluminosos, cálcio-alcalinos de alto a médio K, formados entre 630 a 620 Ma em ambiente de margem continentalativa. As Unidades Piraí do Sul e Santa Rita do BCP são constituídas por monzogranitos fracamente peraluminosos, de altoK e as Unidades São Sebastião e Conceição do BTC são constituídas respectivamente por quartzo-monzonitos e monzo asienogranitos fracamente metaluminosos, de alto K. Estas unidades foram formadas em ambiente sin- a tardi-colisional entre620 e 590 Ma.

  13. Abundance and Monthly Frequency of Phlebotomine Sand Flies (Diptera: Phlebotominae in Some Municipalities in the State of Rio de Janeiro, Brazil

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    Alfredo Carlos Azevedo

    2015-12-01

    Resumo. O presente estudo teve o objetivo de estimar a abundância e frequência mensal de algumas espécies de flebotomíneos incriminados como vetores da Leishmaniose Tegumentar Americana (LTA, nos municípios de Saquarema, Rio Bonito, Piraí e Rio Janeiro, no Estado do Rio de Janeiro. Sete sítios localizados nesses municípios foram monitorados ao longo de um período de 10 a 12 meses. Lutzomyia migonei (França, Lutzomyia (Pintomyia fischeri (Pinto e Lutzomyia (Nyssomyia intermedia (Lutz & Neiva, foram registrados em todos os quatro municípios. Lutzomyia (Lutzomyia longipalpis (Lutz & Neiva vetor comprovado da Leishmaniose Visceral (LV, foi registrado. Além desses vetores, outras três espécies de flebotomíneos, incluindo Lutzomyia edwardsi (Mangabeira, Lutzomyia sallesi (Galvão & Coutinho e Lutzomyia firmatoi (Barreto, Martins & Pellegrino foram capturados em ambiente domiciliar nos sete sítios de monitoramento (SM durante um período de 1.476 horas totalizando 23.187 espécimes. Espécies responsáveis pela transmissão das leishmanioses totalizaram 99,6%. L. (N. intermedia apresentou a maior abundância (SISA = 1,0 e foi registrada em todos os SM, com os maiores valores médios de captura. Lutzomyia migonei foi a segunda mais abundante e também foi encontrada em todos os locais, exceto MS6 (SISA = 0,66. Lutzomyia (L. longipalpis, Lutzomyia sallesi, Lutzomyia firmatoi e Lutzomyia (P. fischeri foram ocasionalmente observadas nas SM. Esses estudos apontam para a necessidade de adoção de políticas que envolvam ações de educação em saúde, associados à noção de manejo ambiental e conceitos básicos da doença, como elemento de sucesso de um programa integrado de vigilância entomológica e controle da LTA.

  14. Rossendorf Beamline at ESRF (ROBL-CRG). Bi-annual report 2009/2010

    Energy Technology Data Exchange (ETDEWEB)

    Scheinost, Andreas C.; Baehtz, Carsten (eds.)

    2011-07-01

    be continued until May 2012, since the ESRF shuts down for a major upgrade from December 2011 to April 2012. We expect that we will be ready for user operation from June 2012 on, with a better beamline than ever. The beamline staff would like to thank all partners, research groups and organizations who supported the beamline during the last 24 months. Special thanks to the FZD management, the CRG office of the ESRF with Axel Kaprolat as liaison officer and Eric Dettona as lead technician, and to the ESRF safety group members, Paul Berkvens, Patrick Colomp and Yann Pira.

  15. Diverse Early Life-History Strategies in Migratory Amazonian Catfish: Implications for Conservation and Management.

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    Jens C Hegg

    Full Text Available Animal migrations provide important ecological functions and can allow for increased biodiversity through habitat and niche diversification. However, aquatic migrations in general, and those of the world's largest fish in particular, are imperiled worldwide and are often poorly understood. Several species of large Amazonian catfish carry out some of the longest freshwater fish migrations in the world, travelling from the Amazon River estuary to the Andes foothills. These species are important apex predators in the main stem rivers of the Amazon Basin and make up the region's largest fishery. They are also the only species to utilize the entire Amazon Basin to complete their life cycle. Studies indicate both that the fisheries may be declining due to overfishing, and that the proposed and completed dams in their upstream range threaten spawning migrations. Despite this, surprisingly little is known about the details of these species' migrations, or their life history. Otolith microchemistry has been an effective method for quantifying and reconstructing fish migrations worldwide across multiple spatial scales and may provide a powerful tool to understand the movements of Amazonian migratory catfish. Our objective was to describe the migratory behaviors of the three most populous and commercially important migratory catfish species, Dourada (Brachyplatystoma rousseauxii, Piramutaba (Brachyplatystoma vaillantii, and Piraíba (Brachyplatystoma filamentosum. We collected fish from the mouth of the Amazon River and the Central Amazon and used strontium isotope signatures ((87Sr/(86Sr recorded in their otoliths to determine the location of early rearing and subsequent. Fish location was determined through discriminant function classification, using water chemistry data from the literature as a training set. Where water chemistry data was unavailable, we successfully in predicted (87Sr/(86Sr isotope values using a regression-based approach that related

  16. ANÁLISE SOBRE A INFLUÊNCIA DE GÊNERO NA DISTINÇÃO DOS JULGAMENTOS DE CRIMES PASSIONAIS

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    VASCONCELOS, R. C.

    2015-12-01

    Full Text Available O crime passional pode ser compreendido como aquele motivado por sentimentos amorosos, sendo justificado por situações diversas como ciúme, defesa da honra, sentimento de posse, entre outros. Os crimes passionais constituem uma categoria particular de delito, sendo caracterizados como aqueles crimes que envolvem uma relação amorosa real ou imaginária entre duas pessoas e que ocorrem a partir de sentimentos de abandono, rejeição ou ciúmes. Sob a justificativa da paixão, tal crime é cometido frequentemente de forma cruel, violenta e atingindo o suposto objeto de amor ou, também, alguém que lhe é muito caro – especialmente os filhos. “Os homicidas passionais trazem em si uma vontade insana de autoafirmação. Ele quer acima de tudo, mostrar-se no comando do relacionamento e causar sofrimento a outrem. Sua história de amor é egocêntrica” (Eluf, p.157, 2009. O presente trabalho busca fazer um paralelo entre o histórico dos julgamentos de crimes passionais ocorridos no Brasil em diferentes épocas: o caso da Fera da Penha, o assassinato de Ângela Diniz e os recentes casos da Fera de Barra do Piraí e o assassinato da funkeira Amanda Bueno, estabelecendo a comparação das diferentes concepções atribuídas aos criminosos a partir de seu gênero – masculino ou feminino. Metodologia utilizada envolve uma revisão de literatura. Segundo a ONU, no mínimo 5 mil mulheres são mortas por ano em nome da honra, e, apesar da escassez de dados contabilizados no Brasil, a cada 10 crimes de natureza passional, em média, 7 são cometidos contra mulheres. Esta diferença possivelmente está relacionada a posição inferiorizada em que a mulher ainda é colocada socialmente, como se fosse seu papel aceitar possíveis traições e rejeições em suas relações amorosas de forma natural, diferente do ocorre quando o homem é aquele que sofre a rejeição. Não é esperado que a mulher se comporte de maneira independente, tampouco tenha

  17. Dinâmica da comunidade de macrófitas aquáticas no reservatório de Santana, RJ Dynamics of the aquatic macrophytecommunity in the Santana reservoir in Pirai-RJ

    Directory of Open Access Journals (Sweden)

    R.L.C.M. Pitelli

    2008-01-01

    Full Text Available Apesar da importância na dinâmica dos ecossistemas aquáticos, as macrófitas podem formar densas e extensas colonizações em corpos hídricos cujos equilíbrios ecológicos foram rompidos. Nessas condições, essas plantas promovem uma série de problemas que as tornam alvos de controle. Para elaboração de planos adequados de manejo dessa vegetação, é fundamental o conhecimento das dinâmicas relativas das populações que a compõem. O objetivo deste trabalho foi realizar levantamentos mensais da composição específica da comunidade de macrófitas que coloniza o reservatório de Santana, localizado no município de Piraí/RJ, monitorando 97 pontos georreferenciados, abrangendo toda a lâmina d'água. Foram identificadas 41 espécies, inseridas em 21 famílias botânicas. As famílias Poaceae, Pontederiaceae e Cyperacae foram as que apresentaram os maiores números de espécies ao longo do ano. Salvinia herzogii e Egeria densa apresentaram as maiores notas anuais de colonização do reservatório. As populações de Eichhornia azurea, Brachiaria arrecta e Paspalum repens completaram o grupo das espécies numericamente mais relevantes. As plantas de hábito flutuante tenderam a apresentar populações com padrão de distribuição geográfica casualizado, enquanto as espécies fixadas no sedimento e as submersas apresentaram populações com padrão agregado. Não houve expressivas variações mensais dos valores dos índices de diversidade (H' e de equitabilidade (E' das comunidades de macrófitas aquáticas ao longo do ano. O dendrograma construído com o coeficiente de Odum mostrou uma seqüência lógica dos meses, evidenciando uma definida sucessão de populações divididas em dois grupos de similaridade separados pelo mês de junho. Nessa época, o nível de água do reservatório foi reduzido e o sedimento ficou exposto, favorecendo as espécies de hábito emergente.Despite the importance of aquatic ecosystem dynamics, aquatic

  18. Remoção mecânica de plantas aquáticas: análise econômica e operacional Mechanical removal of aquatic weeds: operational and economic analysis

    Directory of Open Access Journals (Sweden)

    U.R. Antuniassi

    2002-01-01

    Full Text Available A remoção de plantas aquáticas tem sido utilizada como opção ao controle químico e biológico, em razão de restrições ambientais em algumas regiões brasileiras. O objetivo deste trabalho foi desenvolver um modelo para análise econômica e operacional da remoção mecânica de plantas aquáticas, visando realizar estudo econômico comparativo com o controle químico. A operação foi estudada num reservatório de uma usina de bombeamento em Barra do Piraí-RJ. O sistema consiste de retroescavadeiras instaladas em balsas, usadas para cortar as plantas e liberá-las no fluxo de água. Antes da tomada d'água existe uma barreira flutuante que intercepta as plantas, as quais são removidas por um guindaste fixo nas margens. As plantas são armazenadas por algum tempo e depois descartadas. Existe, ainda, um sistema de limpeza das grades da tomada d'água. Dados do volume total de plantas descartadas foram coletados durante 14 meses, assim como foi avaliado o volume de biomassa produzido por área das principais espécies infestantes. A empreiteira que administra o serviço forneceu planilhas de custos e outro parâmetros operacionais. Um modelo foi desenvolvido para calcular custos por hectare de plantas removidas. Os resultados mostraram custo médio mensal de US$ 17.780,28 por hectare. Apesar do alto custo, o sistema de remoção demonstrou capacidade de controlar apenas 4,1% da área infestada no reservatório, na época da coleta dos dados. Simulando dados de uma aplicação de glyphosate, o controle químico custaria apenas 0,23% do custo da remoção. Análises de sensibilidade mostraram que a compactação das plantas para transporte, o volume de plantas produzidas por área e o custo do transporte são os parâmetros principais para a otimização.Mechanical removal of aquatic weeds has been an option to biological and chemical control because of environmental restrictions in some places in Brazil. The objective of this study was to

  19. Selected Abstracts of the 10th International Workshop on Neonatology; Cagliari (Italy; October 22-25, 2014

    Directory of Open Access Journals (Sweden)

    --- Various Authors

    2014-06-01

    (Italy ABS 9. Traffiking of thymosin beta-4 in the cellular compartments of HepG2 cells at different conditions • M. Piras, M. Piludu, G. Pichiri, P. Coni, T. Cabras, R. Ambu, M. Castagnola; Cagliari and Rome (Italy ABS 10. Thyroid carcinoma in sardinian children and adolescents is often associated with autoimmune thyroiditis • P. Coni, E. Tamponi, A. Lampis, G. Pinna, A.M. Nurchi, S. Farci, M.L. Lai; Cagliari (Italy ABS 11. Prolonging nephrogenesis in preterm infants: a new approach for the prevention of renal disease in adulthood? • A. Sanna, C. Gerosa, D. Fanni, P. Van Eyken, M. Castagnola, R. Ambu; Cagliari, Rome (Italy and Leuven (Belgium ABS 12. Human adrenal gland development: morphogenesis and molecular mechanisms • E. Obinu, V. Fanos, A. Dessì, R. Ambu; Cagliari (Italy ABS 13. Biometrics application for newborns safe identification in Neonatal Intensive Care Units • A. Casanova, S. Barra, M. De Marsico, D. Riccio, L. Barberini, M. Puddu, V. Fanos; Cagliari, Rome and Naples (Italy ABS 14. Metabolomics in neonatal hypoxic-ischemic encephalopathy (HIE: a proposal for a european multicenter study • P. Gancia, G. Pomero, A. Delogu, C. Dalmazzo, G. Palmas, V. Fanos; Cuneo and Cagliari (Italy ABS 15. Longitudinal evaluation of myocardial function in infants with Hypoxic-Ischemic Encephalopathy (HIE: preliminary data • F. Vitali, S. Galletti, A. Aceti, L. Ridolfi, G. Aquilano, M. Fabi, G. Faldella; Bologna (Italy ABS 16. Changes in podocyte number during intrauterine development • M. Desogus, A. Crobe, A. Sanna, C. Gerosa, C. Fanni, C. Loddo, M. Puddu, G. Ottonello, D. Fanni; Cagliari (Italy ABS 17. Efficacy of recombinant human erythropoietin in anemic pregnant women with heterozygous beta-thalassemia • S. Angioni, V. Mais, G.B. Melis; Cagliari (Italy ABS 18. WT1 expression in ontogenesis of human fetus • R. Ambu, L. Vinci, C. Gerosa, D. Fanni, E. Obinu, A. Faa, V. Fanos; Cagliari (Italy ABS 19. Brain hypothermia in perinatal arterial ischemic

  20. Response of the diatom flora in the Gdańsk Basin (southern Baltic Sea) to eutrophication in the last century

    Science.gov (United States)

    Witak, Małgorzata

    2016-04-01

    index nD=1.73). The analysis was performed with a NIKON microscope under a 100× oil immersion objective. The raw counts were transformed to relative abundance of the total frustules counted. The diatoms were divided into groups according to their biotype, salinity, trophy and saproby requirements. The content (in percentage) of all ecological groups were counted in the core. A total of 145 diatom species including varieties and forms representing 53 genera were identified in all samples. The diatom community was strong dominance by small-size planktic euhalobous (Thalassiosira levanderi, Pauliella taeniata) and mesohalobous (Cyclotella choctawhatcheeana) species. They were accompanied by freshwater eutraphentic and pollution-tolerant forms Actinocyclus normanii, Cyclostephanos dubius, Cyclotella atomus, C. caspia, C. meneghiniana, Stephanodiscus hantzschii, S. medius, S. neoastraea and S. parvus. The assemblage structure is a visible evidence of the progressive anthropopressure recorded in the near-bottom sediments of the Gdańsk Basin. Moreover, changes in spatial distribution of diatom anthropogenic assemblage show the close relationship to the distance from mouth of the Vistula River.

  1. New Curculionoidea (Coleoptera records for Canadа

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    Hume Douglas

    2013-06-01

    Full Text Available The following species of Curculionoidea are recorded from Canada for the first time, in ten cases also representing new records at the generic level: Ischnopterapion (Ischnopterapion loti (Kirby, 1808; Stenopterapion meliloti (Kirby, 1808 (both Brentidae; Atrichonotus taeniatulus (Berg, 1881; Barinus cribricollis (LeConte, 1876; Caulophilus dubius (Horn, 1873; Cionus scrophulariae (Linnaeus, 1758; Cryptorhynchus tristis LeConte, 1876; Cylindrocopturus furnissi Buchanan, 1940; Cylindrocopturus quercus (Say, 1832; Desmoglyptus crenatus (LeConte, 1876; Pnigodes setosus LeConte, 1876; Pseudopentarthrum parvicollis (Casey, 1892; Sibariops confinis (LeConte, 1876; Sibariops confusus (Boheman, 1836; Smicronyx griseus LeConte, 1876; Smicronyx lineolatus Casey, 1892; Euwallacea validus (Eichhoff, 1875; Hylocurus rudis (LeConte, 1876; Lymantor alaskanus Wood, 1978; Phloeotribus scabricollis (Hopkins, 1916; Scolytus oregoni Blackman, 1934; Xyleborus celsus Eichhoff, 1868; Xyleborus ferrugineus (Fabricius, 1801; Xylosandrus crassiusculus (Motschulsky, 1866 (all Curculionidae. In addition the following species were recorded for the first time from these provinces and territories: Yukon – Dendroctonus simplex LeConte, 1868; Phloetribus piceae Swaine, 1911 (both Curculionidae; Northwest Territories – Loborhynchapion cyanitinctum (Fall, 1927 (Brentidae; Nunavut – Dendroctonus simplex LeConte, 1868 (Curculionidae; Alberta – Anthonomus tectus LeConte, 1876; Promecotarsus densus Casey, 1892; Dendroctonus ponderosae Hopkins, 1902; Hylastes macer LeConte, 1868; Rhyncolus knowltoni (Thatcher, 1940; Scolytus schevyrewi Semenov Tjan-Shansky, 1902 (all Curculionidae; Saskatchewan – Phloeotribus liminaris (Harris, 1852; Rhyncolus knowltoni (Thatcher, 1940; Scolytus schevyrewi Semenov Tjan-Shansky, 1902 (all Curculionidae; Manitoba – Cosmobaris scolopacea Germar, 1819; Listronotus maculicollis (Kirby, 1837; Listronotus punctiger LeConte, 1876; Scolytus

  2. Conceptual rainfall-runoff model with limited and low quality data in the Pirai River basin, Bolivia

    Science.gov (United States)

    Gonzalez-Sanchez, Alvaro; Villazon, Mauricio; Willems, Patrick

    2010-05-01

    In zones where limited data to apply a distributed hydrological model is available, a combination of lumped conceptual models with channel routing may be a useful tool in the support system for catchment planning. In this approach the whole basin is divided into sub basins, and for each sub basin a lumped conceptual model is built-up. In the present case study the lumped model is NAM and the GIS environment is performed by MIKE BASIN which introduces the hydrological modeling tools into ArcGIS 9.2. Therefore in the Bolivian Amazon, specifically in the Piraí river basin, a confluent of the Amazon River, the MIKE BASIN has been applied for the hydrologic modeling of the gauged sub catchments at daily basis starting in January, 1st 1987 to December 31st 1995. Nevertheless, due to the low quality data, routines in the data have been executed first. The temperature-based methods proposed by Thornthwaite and Hargraves-Samani have been calibrated to local conditions for the evaluation of the reference evapotranspiration (ETo). A rating curve analysis has also been performed in order to complete the discharge series and to correct clearly identifiable errors. SEARPI (River Flood Channeling and Control Service) has been the main source for the data base, it consisted of hourly time series of precipitation for 16 stations and daily time series for 25 stations, 3 weather stations with full climatic data, 6 weather stations with only mean daily temperature values and hourly records of water levels for 4 gauging stations. In general, Hargreaves yields to a more real ETo comparing with the FAO56 than the Thornthwaite method, and could be to the inclusion of the thermal amplitude. However, the aerodynamic factors cannot be fully approximated by the thermal amplitude as it is the case of Viru Viru station. Calculating the maximum and minimum temperature based on other stations may lead to extra uncertainty, taking into account the local range of the mean temperature and latitude

  3. Aborto em bovinos devido à intoxicação por Tetrapterys acutifolia (Malpighiaceae

    Directory of Open Access Journals (Sweden)

    Saulo A. Caldas

    2011-09-01

    Full Text Available Esse estudo teve por objetivo demonstrar experimentalmente que Tetrapterys acutifolia Cav. (fam. Malpighiaceae é capaz de provocar aborto em bovinos e caracterizar as alterações clínico-patológicas nas vacas e nos fetos. Estas plantas são responsáveis por significativo número de mortes em bovinos com mais de um ano de idade, especialmente nos Estados de Rio de Janeiro e São Paulo, mas até agora não havia sido comprovado experimentalmente seu efeito abortivo em bovinos. Os experimentos foram realizados no município de Barra do Piraí, RJ. Quatro vacas de descarte receberam brotos e folhas novas frescas de T. acutifolia, coletadas em propriedades vizinhas, nas doses de 2,5g/kg/dia, 5,0g/kg/dia (2 vacas e 10g/kg/dia, até ocorrer o abortamento. O quadro clínico nas vacas caracterizou-se por arritmia cardíaca, tremores musculares, anorexia, ascite, jugular ingurgitada, edema de peito e barbela e aborto (23-76 dias após o início da ingestão da planta; todas as vacas abortaram. Das quatro vacas apenas uma (a que recebeu 10g/kg/dia morreu 36 dias após o abortamento, com sintomas de insuficiência cardíaca. O exame necroscópico dos fetos/natimortos revelou hidrotórax, hidropericárdio, hidroperitônio e congestão hepática; ao corte do miocárdio, verificaram-se áreas pálidas. No exame histológico havia edema intersticial com fibrose incipiente. Na vaca que recebeu a maior dose e foi a óbito, bem como em outra intoxicada naturalmente, os achados de necropsia foram similares aos observados nos fetos, exceto pela dilatação dos vasos da base do coração e mais acentuada palidez do miocárdio. Observaram-se ainda edema subcutâneo nas regiões cervical e esternal, bem como veias jugulares ingurgitadas. Os achados histopatológicos foram necrose e edema intersticial com acentuada fibrose no miocárdio, espongiose da substância branca do encéfalo e, no fígado, congestão e leve fibrose. Adicionalmente, observou-se na vaca

  4. New exposure ages for the Last Glacial Cycle in the Sanabria Lake region (northwestern Spain)

    Science.gov (United States)

    Rodríguez-Rodríguez, Laura; Jiménez-Sánchez, Montserrat; Domínguez-Cuesta, María Jose; Rinterknecht, Vincent; Pallàs, Raimon; Braucher, Régis; Bourlès, Didier; Valero-Garcés, Blas

    2013-04-01

    ., 2011. Last deglaciation in northwestern Spain: New chronological and geomorphologic evidence from the Sanabria region. Geomorphology 135, 48-65. Palacios, D., Andrés, N., Úbeda, J., Alcalá, J., Marcos, J., Vázquez-Selem, L., 2012. The importance of poligenic moraines in the paleoclimatic interpretation from cosmogenic dating. Geophysical Research Abstracts 14, EGU2012-3759-1. Pérez-Alberti, A., Valcárcel-Díaz, M., Martini, I.P., Pascucci, V., Andrucci, S., 2011. Upper Pleistocene glacial valley-junction sediments at Pias, Trevinca Mountains, NW Spain. In: Martini, I.P., French, H.M., Pérez-Alberti, A. (Eds.), Ice-Marginal and Periglacial Processes and Sediments. Geological Society (London) Special Publication 354, pp. 93-110. Research funded by the projects LIMNOCLIBER (REN2003-09130-C02-02), IBERLIMNO (CGL2005-20236-E/CLI), LIMNOCAL (CGL2006-13327-C04-01) and GRACCIE (CSD2007-00067) of the Spanish Inter-Ministry Commission of Science and Technology (CICYT). Additional funding was provided by the Fundación Patrimonio Natural de Castilla y León through the project "La investigacion en el Lago de Sanabria dentro del proyecto CALIBRE: perspectivas y posibilidades", and by the projects Consolider Ingenio 2006 (CSD2006-0041, Topo-Iberia), 2003 PIRA 00256, HF02.4, and RISKNAT (2009SGR520). L. Rodríguez-Rodríguez has developed her research under a Severo Ochoa Programme fellowship (FICYT- Asturias).

  5. Nutrient Dynamics over the Past 100 Years and Its Restoration Baseline in Dianshan Lake%淀山湖百年营养演化历史及营养物基准的建立

    Institute of Scientific and Technical Information of China (English)

    李小平; 陈小华; 董旭辉; 董志; 孙敦平

    2012-01-01

    Cyclotella bodanica,C.ocelata,Achnanthes minutissima,Cocconeis placentula var lineate,Cymbella sp.,Fragilaria piata,F.brevistrata,F.construens var venter to recent eutrophic species including Cyclostephanos dubius,C.atomus,Stephanodiscus minitulus,S.hantzschi,Aulacoseria alpigena.The epilimnetic TP over the past 100 years reconstructed using an established diatom-TP transfer function matches well with the monitoring TP where exists.Based on the sedimentary nutrient characteristics and diatom-reconstructed nutrient dynamics,we proposed that the nutrient baseline for Dianshan Lake is 50-60 μg·L-1,500 mg·kg-1 and 550 mg·kg-1 for water TP concentration,sedimentary TP and TN,respectively.

  6. A AMBIGUIDADE DO AFETO: CRIMES PASSIONAIS E SUAS DIFERENTES MANIFESTAÇÕES

    Directory of Open Access Journals (Sweden)

    ALMEIDA, C.C.

    2015-12-01

    atingir e ferir o suposto objeto de amor começa a tomar características próprias, com o desejo de ferir este indivíduo da mesma forma que se está sofrendo. Essa reação nem sempre atinge o parceiro diretamente, mas pode atingir alguém que seja extremamente importante para a vítima, fato que teria força suficiente para causar imenso sofrimento. Essas duas características podem ser observadas em dois eventos: o conhecido caso da “Fera da Penha” e o recente caso da manicure de Barra do Piraí. Ambos são casos de crimes planejados sistematicamente que tiveram por principal objetivo atingir seu objeto de suposto amor através da agressão a quem lhe é mais caro – os filhos. É notório, em ambos os casos, a premeditação dos crimes e os atos de barbárie em nome do que os criminosos tinham como idealização de amor, porém, também houve a garantia de que a dor sentida fosse a do luto, causando assim o mesmo sentimento de perda vivenciado pelo autor do crime. Conclusões: O crime passional apresenta aspectos próprios e seu intento é sempre causar dor e profundo sofrimento no objeto de amor, seja sob a forma do assassinato e/ou tortura do mesmo, ou de alguém ou algo de extrema importância na vida do indivíduo, fato que, por si só, possui força suficiente para gerar infinita dor. Tal ato pode ser cometido impulsivamente, porém a literatura vem sistematicamente apontando para crimes que são planejados e executados com requintes de crueldade.

  7. Selected Abstracts of the 9th International Workshop on Neonatology; Cagliari (Italy; October 23-26, 2013

    Directory of Open Access Journals (Sweden)

    --- Various Authors

    2013-06-01

    Full Text Available Selected Abstracts of the 9th International Workshop on Neonatology • LEARNED LESSONS, CHANGING PRACTICE AND CUTTING-EDGE RESEARCH • Cagliari (Italy • October 23rd-26th 2013The Workshop has been organized on behalf of Union of European Neonatal and Perinatal Societies, Union of Mediterranean Neonatal Societies, Italian Society of Neonatology, UNICEF, and under the High Patronage of the President of the Italian Republic. ABS 1. Aetiology and type of reference hospital for neonatal transport in Greece during 2012 • S. Mouskou, C. Varakis, D. Pyrros, N. Iacovidou; Athens (Greece ABS 2. Changes in neonatal outcomes of very low birth weight infants in Hospital of Lithuanian University of Health Sciences • K. Stuikiene, R. Tameliene, D. Stoniene, A. Kudreviciene, V. Ivanauskiene; Kaunas (Lithuania ABS 3. Autoimmune liver disease in childhood • Y. Gibo, D. Fanni, P. Van Eyken, S. Nemolato, G. Floris, C. Gerosa; Matsumoto (Japan, Leuven (Belgium and Cagliari (Italy ABS 4. Lactoferrin attenuates intestinal injury afther perinatal hypoxia and hypothermia • N. Barisic, G. Konstantinidis, V. Stojanovic, A. Doronjski, S. Spasojevic; Novi Sad (Serbia ABS 5. Osteopenia in prematurity: case report • K. Stuikiene, R. Tameliene, D. Stoniene, A. Kudreviciene, V. Ivanauskiene; Kaunas (Lithuania ABS 6. Neonatal Bartter syndrome: case report of a very unusual entity • A. Koutroumpa, K. Georgiou, H. Georgaki, F. Anatolitou; Athens (Greece ABS 7. Placental Growth Factor (PlGF and placental function • I. Atzeni, S.F. Deiana, A. Meloni, B. Piras, P. Zedda, S. Soddu, G. Parodo, G. Faa, G.B. Melis, A.M. Paoletti; Cagliari (Italy ABS 8. Neonatal ECMO: initial experience of Hospital de São João • G. Rocha, P. Soares, T. Henriques-Coelho, J. Correia-Pinto, J. Monteiro, H. Guimarães, R. Roncon-Albuquerque Jr; Porto (Portugal ABS 9. GCMS-based metabolomics analysis of urines in hypoxic neonatal piglets • C. Fattuoni, L. Barberini, N. Iacovidou, T