Phase 2 sampling and analysis plan, Quality Assurance Project Plan, and environmental health and safety plan for the Clinch River Remedial Investigation: An addendum to the Clinch River RCRA Facility Investigation plan

Energy Technology Data Exchange (ETDEWEB)

Cook, R.B.; Adams, S.M.; Beauchamp, J.J.; Bevelhimer, M.S.; Blaylock, B.G.; Brandt, C.C.; Etnier, E.L.; Ford, C.J.; Frank, M.L.; Gentry, M.J.; Greeley, M.S.; Halbrook, R.S.; Harris, R.A.; Holladay, S.K.; Hook, L.A.; Howell, P.L.; Kszos, L.A.; Levine, D.A.; Skiles, J.L.; Suter, G.W.

1992-12-01

This document contains a three-part addendum to the Clinch River Resource Conservation and Recovery Act (RCRA) Facility Investigation Plan. The Clinch River RCRA Facility Investigation began in 1989, as part of the comprehensive remediation of facilities on the US Department of Energy Oak Ridge Reservation (ORR). The ORR was added to the National Priorities List in December 1989. The regulatory agencies have encouraged the adoption of Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) terminology; therefore, the Clinch River activity is now referred to as the Clinch River Remedial Investigation (CRRI), not the Clinch River RCRA Facility Investigation. Part 1 of this document is the plan for sampling and analysis (S A) during Phase 2 of the CRRI. Part 2 is a revision of the Quality Assurance Project Plan for the CRRI, and Part 3 is a revision of the Environmental Health and Safety Plan for the CRRI. The Clinch River RI (CRRI) is designed to address the transport, fate, and distribution of waterborne contaminants (radionuclides, metals, and organic compounds) released from the DOE Oak Ridge Reservation (ORR) and to assess potential risks to human health and the environment associated with these contaminants. Primary areas of investigation are Melton Hill Reservoir, the Clinch River from Melton Hill Dam to its confluence with the Tennessee River, Poplar Creek, and Watts Bar Reservoir. The contaminants identified in the Clinch River/Watts Bar Reservoir (CR/WBR) downstream of the ORR are those associated with the water, suspended particles, deposited sediments, aquatic organisms, and wildlife feeding on aquatic organisms. The purpose of the Phase 2 S A Plan is to describe the proposed tasks and subtasks developed to meet the primary objectives of the CRRI.

Numerical Investigation of Springback in Mechanical Clinching Process

Directory of Open Access Journals (Sweden)

Mohanna Eshtayeh

2017-12-01

Full Text Available In this work, a numerical investigation was conducted to study the springback phenomena in the mechanical clinching process. The springback values were calculated using finite element simulations and it was found that these values depend strongly on the strength of the materials. A Taguchi optimization method was used to determine the optimal parameters affecting springback. However, in the case of materials with low tensile strength, determining parameters affecting springback becomes difficult. Implicit and explicit simulations of clinching joints using the springback analysis show that the distance between the joint sheets becomes almost zero after stress recovery.

Clinch River breeder project gets boost

International Nuclear Information System (INIS)

Hill, W.H.

1982-01-01

Progress on the Clinch River Breeder Reactor Plant project, the United States' next step in developing liquid metal fast breeder technology is examined including consideration of Plant design, component fabrication and testing, construction schedule, funding, fuel cycle development and licensing. (U.K.)

Survival of Seasonal Flooding in the Amazon by the Terrestrial Insect Conotrachelus dubiae O'Brien & Couturier (Coleoptera: Curculionidae), a Pest of the Camu-Camu Plant, Myrciaria dubia (Myrtaceae).

Science.gov (United States)

Delgado, C; Couturier, G; Fine, P V A

2014-08-01

The weevil Conotrachelus dubiae O'Brien & Couturier (Coleoptera: Curculionidae) is a pest of an economically important Amazonian fruit tree Myrciaria dubia (Myrtaceae). This tree grows in seasonally flooded environments, and how weevil larvae survive flooding has not been studied. From December 2004 to May 2009, five experiments were conducted in natural conditions and in the laboratory, with the aim of understanding the mechanisms that allow the survival of C. dubiae larvae in seasonal floods in Amazonia. The larvae of C. dubiae were kept under water for over 93 days. Older instars exposed to periodic circulation of water survived better than younger instars in addition to all larvae that were kept continuously under uncirculated water. Individuals that were collected from plots of M. dubia located in flooded soils and non-flooded soils did not exhibit statistically significant differences in their levels of survival indicating that the variation in survival of flooding events is due to phenotypic plasticity of the species and not to local adaptation by the populations in different environments. We speculate that larvae can survive floods without major physiological changes as larvae appear to obtain oxygen from water by cutaneous diffusion, assisted by caudal movements.

Mechanical clinching process stress and strain in the clinching of EN-AW5754 (AlMg3, and EN AW-5019 (AlMg5 metal plates

Directory of Open Access Journals (Sweden)

J. Cumin

2018-01-01

Full Text Available This paper presents the results of Finite Element Method numerical simulation performed onEN-AW5754(AlMg3, EN AW-5019 (AlMg5 plates subjected to mechanical clinching. The goal was to observe differences between aluminum plates in the same tool; and to determine the possibility of using the constructed tool for the clinching of Al-Al material combinations. This tool construction is to be produced and tested in laboratory conditions, to elaborate prospective results, and reach additional conclusions.

CASE STUDY CRITIQUE; UPPER CLINCH CASE STUDY

Science.gov (United States)

Case study critique: Upper Clinch case study (from Research on Methods for Integrating Ecological Economics and Ecological Risk Assessment: A Trade-off Weighted Index Approach to Integrating Economics and Ecological Risk Assessment). This critique answers the questions: 1) does ...

Health risks from radionuclides released into the Clinch River

International Nuclear Information System (INIS)

Thomas, B.A.; Hoffman, F.O.; Miller, L.F.

1999-01-01

The purpose of this work is to estimate off-site radiation doses and health risks (with uncertainties) associated with the release of radionuclides from the X-10 site. Following an initial screening analysis, the exposure pathways of interest included fish ingestion, drinking water ingestion, the ingestion of milk and meat, and external exposure from shoreline sediment. Four representative locations along the Clinch River, from the White Oak Creek Embayment to the city of Kingston, were chosen. The demography of the lower Clinch River supplied information dealing with land use that aided in the determination of sites on which to focus efforts. The locations that proved to be the most significant included Jones Island at Clinch River Mile (CRM) 20.5, Grassy Creek and K-25 (CRM 14), Kingston Steam Plant (CRM 3.5), and the city of Kingston (CRM 0). These areas of interest have historically been and are still primarily agricultural and residential areas. Reference individuals were determined with respect to the pathways involved. The primary radionuclides of interest released from the X-10 facility into the Clinch River via White Oak Creek were identified in the initial screening analysis as 137 Cs, 90 Sr, 60 Co, 106 Ru, 144 Ce, 131 I, 95 Zr, and 95 Nb. Of these radionuclides, 137 Cs, 60 Co, 106 Ru, 90 Sr, 144 Ce, 95 Zr, and 95 Nb were evaluated for their contribution to the external exposure pathway. This study utilized an object-oriented modeling software package that provides an alternative to the spreadsheet, providing graphical influence diagrams to show qualitative structure of models, hierarchical models to organize complicated models into manageable modules, and intelligent arrays with the power to scale up simple models to handle large problems. The doses and risks estimated in this study are not significant enough to cause a detectable increase in health effects in the population. In most cases, the organ does are well below the limits of epidemiological

Frequency of inhibitors of daphnid trypsin in the widely distributed cyanobacterial genus Planktothrix

DEFF Research Database (Denmark)

Rohrlack, T.; Christoffersen, K.; Friberg-Jensen, U.

2005-01-01

on the frequency of such compounds in the widely distributed cyanobacterial genus Planktothrix. Of the 89 Planktothrix strains analysed, about 70% produced inhibitors of daphnid trypsin. The strains tested positive represented three common Planktothrix species and were isolated from diverse localities...

Interaction of chemical cues from fish tissues and organophosphorous pesticides on Ceriodaphnia dubia survival

International Nuclear Information System (INIS)

Maul, Jonathan D.; Farris, Jerry L.; Lydy, Michael J.

2006-01-01

Cladocera are frequently used as test organisms for assessing chemical and effluent toxicity and have been shown to respond to stimuli and cues from potential predators. In this study, the interactive effects of visual and chemical cues of fish and two organophosphorous pesticides on survival of Ceriodaphnia dubia were examined. A significant chemical cue (homogenized Pimephales promelas) and malathion interaction was observed on C. dubia survival (P = 0.006). Chemical cue and 2.82 μg/L malathion resulted in a 76.0% reduction in survival compared to malathion alone (P < 0.01). Furthermore, potentiation of malathion toxicity varied based on the source of chemical cues (i.e., epithelial or whole body). It is unclear in this study whether these chemical cues elicited a predation-related stress in C. dubia. Future research should examine the mechanism of this interaction and determine what role, if any, stress responses by C. dubia might play in the interaction. - Potentiation of organophosphorous pesticide toxicity to Ceriodaphnia dubia by fathead minnow (Pimephales promelas) chemical cues was observed

Regulation and dysregulation of vitellogenin mRNA accumulation in daphnids (Daphnia magna)

Energy Technology Data Exchange (ETDEWEB)

Hannas, Bethany R.; Wang, Ying H.; Thomson, Susanne; Kwon, Gwijun; Hong, Li [Department of Environmental and Molecular Toxicology, North Carolina State University, Raleigh, NC 27695-7633 (United States); LeBlanc, Gerald A., E-mail: Gerald_LeBlanc@ncsu.edu [Department of Environmental and Molecular Toxicology, North Carolina State University, Raleigh, NC 27695-7633 (United States)

2011-01-25

The induction of vitellogenin in oviparous vertebrates has become the gold standard biomarker of exposure to estrogenic chemicals in the environment. This biomarker of estrogen exposure also has been used in arthropods, however, little is known of the factors that regulate the expression of vitellogenin in these organisms. We investigated changes in accumulation of mRNA products of the vitellogenin gene Vtg2 in daphnids (Daphnia magna) exposed to a diverse array of chemicals. We further evaluated the involvement of hormonal factors in the regulation of vitellogenin expression that may be targets of xenobiotic chemicals. Expression of the Vtg2 gene was highly responsive to exposure to various chemicals with an expression range spanning approximately four orders of magnitude. Chemicals causing the greatest induction were piperonyl butoxide, chlordane, 4-nonylphenol, cadmium, and chloroform. Among these, only 4-nonylphenol is recognized to be estrogenic. Exposure to several chemicals also suppressed Vtg2 mRNA levels, as much as 100-fold. Suppressive chemicals included cyproterone acetate, acetone, triclosan, and atrazine. Exposure to the estrogens diethylstilbestrol and bisphenol A had little effect on vitellogenin mRNA levels further substantiating that these genes are not induced by estrogen exposure. Exposure to the potent ecdysteroids 20-hydroxyecdysone and ponasterone A revealed that Vtg2 was subject to strong suppressive control by these hormones. Vtg2 mRNA levels were not significantly affected from exposure to several juvenoid hormones. Results indicate that ecdysteroids are suppressors of vitellogenin gene expression and that vitellogenin mRNA levels can be elevated or suppressed in daphnids by xenobiotics that elicit antiecdysteroidal or ecdysteroidal activity, respectively. Importantly, daphnid Vtg2 is not elevated in response to estrogenic activity.

Regulation and dysregulation of vitellogenin mRNA accumulation in daphnids (Daphnia magna)

International Nuclear Information System (INIS)

Hannas, Bethany R.; Wang, Ying H.; Thomson, Susanne; Kwon, Gwijun; Li Hong; LeBlanc, Gerald A.

2011-01-01

The induction of vitellogenin in oviparous vertebrates has become the gold standard biomarker of exposure to estrogenic chemicals in the environment. This biomarker of estrogen exposure also has been used in arthropods, however, little is known of the factors that regulate the expression of vitellogenin in these organisms. We investigated changes in accumulation of mRNA products of the vitellogenin gene Vtg2 in daphnids (Daphnia magna) exposed to a diverse array of chemicals. We further evaluated the involvement of hormonal factors in the regulation of vitellogenin expression that may be targets of xenobiotic chemicals. Expression of the Vtg2 gene was highly responsive to exposure to various chemicals with an expression range spanning approximately four orders of magnitude. Chemicals causing the greatest induction were piperonyl butoxide, chlordane, 4-nonylphenol, cadmium, and chloroform. Among these, only 4-nonylphenol is recognized to be estrogenic. Exposure to several chemicals also suppressed Vtg2 mRNA levels, as much as 100-fold. Suppressive chemicals included cyproterone acetate, acetone, triclosan, and atrazine. Exposure to the estrogens diethylstilbestrol and bisphenol A had little effect on vitellogenin mRNA levels further substantiating that these genes are not induced by estrogen exposure. Exposure to the potent ecdysteroids 20-hydroxyecdysone and ponasterone A revealed that Vtg2 was subject to strong suppressive control by these hormones. Vtg2 mRNA levels were not significantly affected from exposure to several juvenoid hormones. Results indicate that ecdysteroids are suppressors of vitellogenin gene expression and that vitellogenin mRNA levels can be elevated or suppressed in daphnids by xenobiotics that elicit antiecdysteroidal or ecdysteroidal activity, respectively. Importantly, daphnid Vtg2 is not elevated in response to estrogenic activity.

Bioaccumulation of Fe2O3(magnetic) nanoparticles in Ceriodaphnia dubia

International Nuclear Information System (INIS)

Hu Ji; Wang Demin; Wang Jiangtao; Wang Jianmin

2012-01-01

While nano-Fe 2 O 3 (magnetic) is generally considered non-toxic, it could serve as a carrier of other toxic chemicals such as As(V) and enhance their toxicity. The bioaccumulation of nano-Fe 2 O 3 (m) with different exposure times, NP concentrations, and pH conditions was investigated using Ceriodaphnia dubia (C. dubia) as the model organism. Under natural pH conditions, C. dubia significantly accumulated nano-Fe 2 O 3 (m) in the gut, with the maximum accumulation being achieved after 6 h of exposure. The concentration of nano-Fe 2 O 3 also impacted its accumulation, with the maximum uptake occurring at 20 mg/L or more. In addition, the highest bioaccumulation occurred in a pH range of 7–8 where the highest feeding rate was reported, confirming that the ingestion of NPs is the main route of nano-Fe 2 O 3 (m) bioaccumulation. In a clean environment without NPs, depuration of nano-Fe 2 O 3 (m) occurred, and food addition accelerated the depuration process. - Highlights: ► Nano-Fe 2 O 3 (m) enhances the toxicity of As(V). ► C. dubia significantly accumulate nano-Fe 2 O 3 (m) through ingestion. ► The bioaccumulation of nano-Fe 2 O 3 (m) is affected by time, NP concentration, and pH. ► Food addition accelerates the depuration process of accumulated nano-Fe 2 O 3 (m). - Nano-Fe 2 O 3 (m) could enhance the toxicity of As(V) due to the significant accumulation of nano-Fe 2 O 3 (m) along with sorbed As(V) by C. dubia through ingestion.

Addendum to the Phase 2 Sampling and Analysis Plan for the Clinch River Remedial Investigation

International Nuclear Information System (INIS)

1994-03-01

This document is an addendum to the Phase 2 Sampling and Analysis Plan for the Clinch River Remedial Investigation (DOE 1993). The Department of Energy--Oak Ridge Operations (DOE-ORO) is proposing this addendum to the US Envianmental Protection Agency, Region IV (EPA-IV), and the Tennessee Department of Environment and Conservation (TDEC) as a reduced sampling program on the Clinch River arm of Watts Bar Reservoir and on Poplar Creek. DOE-ORO is proposing to maximize the use of existing data and minimize the collection of new data for water, sediment, and biota during Phase 2 of the Clinch River Remedial Investigation. The existing data along with the additional data collected in Phase 2 would be used to perform a baseline risk assessment and make remedial decisions. DOE-ORO considers that the existing data, the additional data collected in Phase 2, and on-site remedial investigation data would be sufficient to understand the nature and extent of the contamination problem in the Clinch River, perform a baseline risk assessment,and make remedial decisions. This addendum is organized in three sections. The first section provides background information and describes a rationale for modifying the Phase 2 Sampling and Analysis Plan. Section 2 presents a summary of the existing data for the Clinch River arm of Watts Bar Reservoir and an evaluation of the sufficiency of this data for a baseline human health and ecological risk assessment. Section 3 describes the revised Phase 2 Sampling and Analysis Plan for surface water, sediment, and biota in the Clinch River OU and in the Poplar Creek OU

Saga of Clinch River

International Nuclear Information System (INIS)

Young, W.H.

1984-01-01

An epic struggle in the US Congress between what the author calls the forces of transcendence and the forces of experience over development of a breeder reactor for electric power generation is described in this article. The project was started by President Nixon, survived repeated attacks under President Carter, and ironically succumbed under a strong supporter, President Reagan, as a result of an unlikely coalition of conservative organizations and Republican politicians. The broader meanings of the demise of the Clinch River project are examined on several levels, examining the significance for the nation's energy future and for the nation's political future

Characterization of surface water contaminants in the Clinch River and Poplar Creek

International Nuclear Information System (INIS)

Ford, C.; Madix, S.; Rash, C.

1995-01-01

Surface waters in the Clinch River and Poplar Creek have been contaminated by activities on the DOE's Oak Ridge Reservation throughout the more than 50 year history of Oak Ridge. Though the Clinch River and Poplar Creek drainage areas are contaminated with heavy metals, organics and radionuclides, public access to these sites is not restricted. The investigation, divided into discrete studies, was tailored to provide a statistically sound picture of contaminants and aqueous toxicity in Poplar Creek, investigate contaminant remobilization from sediments, and determine contaminant levels during a series of ''worst-case'' events. Results for Poplar Creek indicate that average contaminant values were below levels of concern for human health and ecological risk, though contaminant distributions suggest that episodic events contribute sufficiently to system contaminant levels to be of concern. Additionally, water column contaminant levels were significantly higher in particle deposition areas rather than at known contaminant sources. Levels of organic compounds in reference areas to Poplar Creek exceeded those in the Poplar Creek study area. In the Clinch River and Poplar Creek, statistical differences in metal and radionuclide levels from known contaminated areas confirmed previous results, and were used to independently distinguish between sites. Contaminant concentrations were elevated in association with sediments, though no distinction between deposition and remobilization could be made. Due to elevated contaminant levels, and some unexpected contaminant distributions, sites in Poplar Creek and off-channel embayments of the Clinch River were identified that will require additional characterization

Numerical modeling of mechanical behavior of clinch connections at breaking out and shearing

Directory of Open Access Journals (Sweden)

Berezhnoi Dmitri V.

2017-01-01

Full Text Available This article describes an approach to constructing the defining relationships between increment of true stresses and true deformations, with considering the contact interaction of elastoplastic deformed bodies among each other. Within the framework of finite element method, solving these problems in case of “breaking out” and “shearing” in the clinch joint, the stress fields in the zone of the clinch connection are defined, and recommendations are given for realizing the process of their creation.

Technical background information for the environmental and safety report, Volume 5: the 1977 Clinch River sediment survey - data presentation

International Nuclear Information System (INIS)

Oakes, T.W.; Ohnesorge, W.F.; Eldridge, J.S.; Scott, T.G.; Parsons, D.W.; Hubbard, H.M.; Sealand, O.M.; Shank, K.E.; Eyman, L.D.

1982-11-01

This study determined the fate and distribution of nuclides in the Clinch River by analyzing selected cores for transuranic radionuclide activity and examined the effect of the altered flow regime in the Clinch River on the distribution of the fission product activity. Cores were collected along the full length of the Clinch River from the WOC outfall (CRM 20.8) to locations in the Tennessee River on either side of the junction of the two rivers. The sampling was concentrated around CRM 20.8 and the proposed CRBR site. An inventory of alpha-emitting radionuclides and gamma-emitting fission products and their lateral and vertical distribution patterns in the Clinch River was established

Isolation of high-quality total RNA from leaves of Myrciaria dubia "CAMU CAMU".

Science.gov (United States)

Gómez, Juan Carlos Castro; Reátegui, Alina Del Carmen Egoavil; Flores, Julián Torres; Saavedra, Roberson Ramírez; Ruiz, Marianela Cobos; Correa, Sixto Alfredo Imán

2013-01-01

Myrciaria dubia is a main source of vitamin C for people in the Amazon region. Molecular studies of M. dubia require high-quality total RNA from different tissues. So far, no protocols have been reported for total RNA isolation from leaves of this species. The objective of this research was to develop protocols for extracting high-quality total RNA from leaves of M. dubia. Total RNA was purified following two modified protocols developed for leaves of other species (by Zeng and Yang, and by Reid et al.) and one modified protocol developed for fruits of the studied species (by Silva). Quantity and quality of purified total RNA were assessed by spectrophotometric and electrophoretic analysis. Additionally, quality of total RNA was evaluated with reverse-transcription polymerase chain reaction (RT-PCR). With these three modified protocols we were able to isolate high-quality RNA (A260nm/A280nm >1.9 and A260nm/A230nm >2.0). Highest yield was produced with the Zeng and Yang modified protocol (384±46µg ARN/g fresh weight). Furthermore, electrophoretic analysis showed the integrity of isolated RNA and the absence of DNA. Another proof of the high quality of our purified RNA was the successful cDNA synthesis and amplification of a segment of the M. dubia actin 1 gene. We report three modified protocols for isolation total RNA from leaves of M. dubia. The modified protocols are easy, rapid, low in cost, and effective for high-quality and quantity total RNA isolation suitable for cDNA synthesis and polymerase chain reaction.

"Wandering in the Desert": The Clinch River Breeder Reactor Debate in the U.S. Congress, 1972-1983.

Science.gov (United States)

Camp, Michael

2018-01-01

The experimental Clinch River breeder reactor, approved by the U.S. Congress in 1970 for construction in East Tennessee, would have used plutonium instead of uranium. The project drew the ire of environmentalists who insisted that plutonium was too dangerous for commercial use, along with opponents of nuclear proliferation. Tennessee's representatives in Congress, however, desired the jobs that the project would create, and formed legislative coalitions to ensure continued appropriations for the project. Funding lasted until 1983, when fiscal conservatives, concerned about ballooning cost projections, joined with environmentalists to defund the breeder. Interpretations of U.S. nuclear policy in the 1980s have often revolved around the Three Mile Island meltdown's aftermath, but Clinch River was not affected by the incident. Instead, the Clinch River controversy revolved around other unrelated issues. The Clinch River story therefore offers a corrective to accounts that privilege national public opinion at the expense of other variables.

Characterization of sediments in the Clinch River, Tennessee, using remote sensing and multi-dimensional GIS techniques

International Nuclear Information System (INIS)

Levine, D.A.; Hargrove, W.W.; Hoffman, F.

1995-01-01

Remotely-sensed hydro-acoustic data were used as input to spatial extrapolation tools in a GIS to develop two- and three-dimensional models of sediment densities in the Clinch River arm of Watts Bar Reservoir, Tennessee. This work delineated sediment deposition zones to streamline sediment sampling and to provide a tool for estimating sediment volumes and extrapolating contaminant concentrations throughout the system. The Clinch River arm of Watts Bar Reservoir has been accumulating sediment-bound contaminants from three Department of Energy (DOE) facilities on the Oak Ridge Reservation, Tennessee. Public concern regarding human and ecological health resulted in Watts Bar Reservoir being placed on the National Priorities List for SUPERFUND. As a result, DOE initiated and is funding the Clinch River Environmental Restoration Program (CR-ERP) to perform a remedial investigation to determine the nature and extent of sediment contamination in the Watts Bar Reservoir and the Clinch River and to quantify any human or ecological health risks. The first step in characterizing Clinch River sediments was to determine the locations of deposition zones. It was also important to know the sediment type distribution within deposition zones because most sediment-bound contaminants are preferentially associated to fine particles. A dual-frequency hydro-acoustic survey was performed to determine: (1) depth to the sediment water interface, (2) depth of the sediment layer, and (3) sediment characteristics (density) with depth (approximately 0.5-foot intervals). An array of geophysical instruments was used to meet the objectives of this investigation

Ecotoxic effect of photocatalytic active nanoparticles (TiO2) on algae and daphnids.

Science.gov (United States)

Hund-Rinke, Kerstin; Simon, Markus

2006-07-01

Due to their large potential for manifold applications, the use of nanoparticles is of increasing importance. As large amounts of nanoparticles may reach the environment voluntarily or by accident, attention should be paid on the potential impacts on the environment. First studies on potential environmental effects of photocatalytic TiO2 nanoparticles have been performed on the basis of widely accepted, standardized test systems which originally had been developed for the characterization of chemicals. The methods were adapted to the special requirements of testing photocatalytic nanoparticles. Suspensions of two different nanoparticles were illuminated to induce their photocatalytic activity. For testing, the growth inhibition test with the green alga Desmodesmus subspicatus and the immobilization test with the daphnid Daphnia magna were selected and performed following the relevant guidelines (algae: ISO 8692, OECD 201, DIN 38412-33; daphnids: ISO 6341, OECD 202, DIN 38412-30). The guidelines were adapted to meet the special requirements for testing photocatalytic nanoparticles. The results indicate that it is principally possible to determine the ecotoxicity of nanoparticles. It was shown that nanoparticles may have ecotoxicological effects which depend on the nature of the particles. Both products tested differ in their toxicity. Product 1 shows a clear concentration-effect curve in the test with algae (EC50: 44 mg/L). It could be proven that the observed toxicity was not caused by accompanying contaminants, since the toxic effect was comparable for the cleaned and the commercially available product. For product 2, no toxic effects were determined (maximum concentration: 50 mg/L). In the tests with daphnids, toxicity was observed for both products, although the concentration effect-curves were less pronounced. The two products differed in their toxicity; moreover, there was a difference in the toxicity of illuminated and non-illuminated products. Both products

DOE's environmental restoration program for the Clinch River and Watts Bar Reservoir

International Nuclear Information System (INIS)

Kimmel, B.

1992-01-01

Operations and waste disposal activities at the Y-12 Plant, the K-25 Site,and the Oak Ridge National Laboratory (ORNL) on the U.S. Department of Energy's Oak Ridge Reservation (ORR) have introduced a variety of contaminants (radionuclides, metals, and organic compounds) into off-site surface waters since the early 1940s, The Clinch River and Watts Bar Reservoir are located downstream from the ORR. A comprehensive remedial investigation (the Clinch River Remedial Investigation) of off-site surface water contamination at Oak Ridge is now being conducted in compliance with the Resource Conservation and Recovery Act and Comprehensive Environmental Response, Compensation, and Liability Act requirements. The objectives of the Clinch River Remedial Investigation (CRRI) are to: (1) define the nature and extent of off-site surface water contamination, (2) quantify the potential risks to human health and the environment associated with off-site contamination, and (3) identify and preliminarily evaluate potential remediation alternatives. The CRRI is being conducted in three phases: (1) scoping studies, in which preassessment studies based on existing data and limited sampling were conducted to preliminarily estimate the nature and extent of the problem; (2) Phase 1, in which limited sampling and risk analyses are conducted to define specifically the distributions of the contaminants of concern and the environmental and human health risks associated with the contamination. These phases allow a progressive focusing of assessment efforts on specific contaminants, pathways, and sites contributing to risk and on the evaluation of potential remediation alternatives. A brief overview of the Clinch River RI is presented, followed by a description of on going efforts to achieve control of contaminated sediments located in the White Oak Creek Embayment

Propiedades prooxidantes del camu camu (Myrciaria dubia

Directory of Open Access Journals (Sweden)

Henry Guija

2005-12-01

Full Text Available Objetivo: Determinar el efecto del ion férrico sobre las propiedades prooxidantes del camu camu (Myrciaria dubia. Diseño: Estudio analítico, experimental, prospectivo y longitudinal. Materiales y Métodos: Se ha evaluado las propiedades prooxidantes del camu camu (Myrciaria dubia, fruto caracterizado por tener un elevado contenido de vitamina C, frente a Fe(III, etilendiamino tetraacético (EDTA, tiourea y manitol. Resultados: El camu camu en presencia de Fe-III en tampón fosfato a pH 7,4 incrementó notablemente la generación de radicales libres a través de una cinética de saturación, efecto que fue dependiente de la concentración del metal. La presencia de tiourea o manitol, compuestos de conocida acción antioxidante, inhibieron la formación de radicales libres, en cambio el EDTA lo incrementó. Conclusión: El camu camu incrementa la generación de radicales libres en presencia de Fe(III y EDTA.

Clinch River Breeder Reactor Plant Project: construction schedule

International Nuclear Information System (INIS)

Purcell, W.J.; Martin, E.M.; Shivley, J.M.

1982-01-01

The construction schedule for the Clinch River Breeder Reactor Plant and its evolution are described. The initial schedule basis, changes necessitated by the evaluation of the overall plant design, and constructability improvements that have been effected to assure adherence to the schedule are presented. The schedule structure and hierarchy are discussed, as are tools used to define, develop, and evaluate the schedule

Antigenotoxic effect of acute, subacute and chronic treatments with Amazonian camu-camu (Myrciaria dubia) juice on mice blood cells.

Science.gov (United States)

da Silva, Francisco Carlos; Arruda, Andrelisse; Ledel, Alexandre; Dauth, Cíntia; Romão, Nathalia Faria; Viana, Rafaele Nazário; de Barros Falcão Ferraz, Alexandre; Picada, Jaqueline Nascimento; Pereira, Patrícia

2012-07-01

Myrciaria dubia, a plant native to the Amazon region, stands out as a fruit rich in vitamin C and other metabolites with nutritional potential. We evaluated the antioxidant, genotoxic and antigenotoxic potential of M. dubia juice on blood cells of mice after acute, subacute and chronic treatments. Flavonoids and vitamin C present in the fruit of M. dubia were quantified. In vitro antioxidant activity was evaluated by DPPH assay. Blood samples were collected for analysis after treatment, and the alkaline comet assay was used to analyze the genotoxic and antigenotoxic activity (ex vivo analysis using H(2)O(2)). The amount of vitamin C per 100mL of M. dubia was 52.5mg. DPPH assay showed an antioxidant potential of the fruit. No M. dubia concentration tested exerted any genotoxic effect on mice blood cells. In the ex vivo test, the juice demonstrated antigenotoxic effect, and acute treatment produced the most significant results. After the treatments, there was no evidence of toxicity or death. In conclusion, our data show that M. dubia juice has antigenotoxic and antioxidant activities, though with no genotoxicity for blood cells. Nevertheless, more in-depth studies should be conducted to assess the safety of this fruit for human consumption. Copyright © 2012 Elsevier Ltd. All rights reserved.

The Clinch Bend Regional Industrial Site and economic development opportunities

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-12-31

This effort focuses initially on the Clinch Bend site. Other sites and developable tracts of land are identified with the assistance of communities in proximity to Oak Ridge, the State of Tennessee, and others, and compared with the projected site requirements for large industrial facilities.

Synergistic toxic effect of nano-Al2O3 and As(V) on Ceriodaphnia dubia

International Nuclear Information System (INIS)

Wang Demin; Hu Ji; Forthaus, Brett E.; Wang Jianmin

2011-01-01

Engineered nanomaterials (ENMs) alone could negatively impact the environment and human health. However, their role in the presence of other toxic substances is not well understood. The toxicity of nano-Al 2 O 3 , inorganic As(V), and a combination of both was examined with C. dubia as the model organisms. Bare nano-Al 2 O 3 particles exhibited partial mortality at concentrations of greater than 200 mg/L. When As(V) was also present, a significant amount of As(V) was accumulated on the nano-Al 2 O 3 surface, and the calculated LC 50 of As(V) in the presence of nano-Al 2 O 3 was lower than that it was without the nano-Al 2 O 3 . The adsorption of As(V) on the nano-Al 2 O 3 surface and the uptake of nano-Al 2 O 3 by C. dubia were both verified. Therefore, the uptake of As(V)-loaded nano-Al 2 O 3 was a major reason for the enhanced toxic effect. - Highlights: → Nano-Al 2 O 3 particles alone do not have significant toxic effect on C. dubia. → However, nano-Al 2 O 3 particles significantly enhance the toxicity of As(V). → The uptake of As-loaded nano-Al 2 O 3 by C. dubia plays the major role on the toxicity. - Nano-Al 2 O 3 could accumulate background As(V) and enhance As(V) toxicity on C. dubia through the uptake of As(V)-loaded nano-Al 2 O 3 particles.

Utility of population models to reduce uncertainty and increase value relevance in ecological risk assessments of pesticides: an example based on acute mortality data for daphnids.

Science.gov (United States)

Hanson, Niklas; Stark, John D

2012-04-01

Traditionally, ecological risk assessments (ERA) of pesticides have been based on risk ratios, where the predicted concentration of the chemical is compared to the concentration that causes biological effects. The concentration that causes biological effect is mostly determined from laboratory experiments using endpoints on the level of the individual (e.g., mortality and reproduction). However, the protection goals are mostly defined at the population level. To deal with the uncertainty in the necessary extrapolations, safety factors are used. Major disadvantages with this simplified approach is that it is difficult to relate a risk ratio to the environmental protection goals, and that the use of fixed safety factors can result in over- as well as underprotective assessments. To reduce uncertainty and increase value relevance in ERA, it has been argued that population models should be used more frequently. In the present study, we have used matrix population models for 3 daphnid species (Ceriodaphnia dubia, Daphnia magna, and D. pulex) to reduce uncertainty and increase value relevance in the ERA of a pesticide (spinosad). The survival rates in the models were reduced in accordance with data from traditional acute mortality tests. As no data on reproductive effects were available, the conservative assumption that no reproduction occurred during the exposure period was made. The models were used to calculate the minimum population size and the time to recovery. These endpoints can be related to the European Union (EU) protection goals for aquatic ecosystems in the vicinity of agricultural fields, which state that reversible population level effects are acceptable if there is recovery within an acceptable (undefined) time frame. The results of the population models were compared to the acceptable (according to EU documents) toxicity exposure ratio (TER) that was based on the same data. At the acceptable TER, which was based on the most sensitive species (C. dubia

Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract.

Science.gov (United States)

Correia, Vanessa Carolina de Sena; Lima, Nathália Oliveira; Oliveira, Flávio Augusto de Souza; Santos, Ana Paula de Azevedo Dos; Teles, Carolina Bioni Garcia; Oliveira, Waldesse Piragé de; Pimenta, And Raphael Sanzio

2016-01-01

Malaria and leishmaniasis are prevalent in tropical regions, which have environmental characteristics that are highly favorable to protozoa and vectors of these diseases; the transmission of these infections in sub-tropical regions, although recognized, represents only a small fraction of cases. Plants are constantly being used in the search for and acquisition of new drugs, and many compounds derived from them have been used to combat various diseases. In this study, we evaluated the action of the dichloromethanolic extract of Myrciaria dubia leaves against the protozoa Plasmodium falciparum, Leishmania amazonensis, Leishmania braziliensis, and Leishmania chagasi through bioassays. The extract from M. dubia was tested for its anti-P. falciparum activity in an anti-histidine-rich protein II immunosorbent assay. The antileishmanial assays were performed using the resazurin method, while cytotoxicity against human hepatoma (HepG2) strain was determined using the colorimetric MTT [3-(4, 5-dimethyl-2- thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide] method. The M. dubia extract presented a half-maximal inhibitory concentration equal to 2.35 (1.05)μg/mL for P. falciparum, 190.73 (6.41) μg/mL for L. amazonensis, and greater than equal to 200µg/mL for L. chagasi and L. braziliensis strains. The cytotoxic concentration for 50% of the cells was above 500μg/mL for HepG2, indicating no toxicity and greater selectivity against parasites. The results obtained indicate the presence of antiplasmodial and leishmanicidal bioactive compounds in the dichloromethanolic extracts of M. dubia leaves, and point towards future studies to elucidate the mechanism of action for each physiological effect.

Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae extract

Directory of Open Access Journals (Sweden)

Vanessa Carolina de Sena Correia

Full Text Available Abstract INTRODUCTION: Malaria and leishmaniasis are prevalent in tropical regions, which have environmental characteristics that are highly favorable to protozoa and vectors of these diseases; the transmission of these infections in sub-tropical regions, although recognized, represents only a small fraction of cases. Plants are constantly being used in the search for and acquisition of new drugs, and many compounds derived from them have been used to combat various diseases. In this study, we evaluated the action of the dichloromethanolic extract of Myrciaria dubia leaves against the protozoa Plasmodium falciparum, Leishmania amazonensis, Leishmania braziliensis, and Leishmania chagasi through bioassays. METHODS The extract from M. dubia was tested for its anti-P. falciparum activity in an anti-histidine-rich protein II immunosorbent assay. The antileishmanial assays were performed using the resazurin method, while cytotoxicity against human hepatoma (HepG2 strain was determined using the colorimetric MTT [3-(4, 5-dimethyl-2- thiazolyl-2, 5-diphenyl-2H tetrazolium bromide] method. RESULTS The M. dubia extract presented a half-maximal inhibitory concentration equal to 2.35 (1.05μg/mL for P. falciparum, 190.73 (6.41 μg/mL for L. amazonensis, and greater than equal to 200µg/mL for L. chagasi and L. braziliensis strains. The cytotoxic concentration for 50% of the cells was above 500μg/mL for HepG2, indicating no toxicity and greater selectivity against parasites. CONCLUSIONS The results obtained indicate the presence of antiplasmodial and leishmanicidal bioactive compounds in the dichloromethanolic extracts of M. dubia leaves, and point towards future studies to elucidate the mechanism of action for each physiological effect.

Antibacterial activity of Myrciaria dubia (Camu camu) against Streptococcus mutans and Streptococcus sanguinis

Institute of Scientific and Technical Information of China (English)

Rosella Camere-Colarossi; Gabriela Ulloa-Urizar; Dyanne Medina-Flores; Stefany Caballero-Garca; Frank Mayta-Tovalino; Juana del Valle-Mendoza

2016-01-01

Objective: To evaluate the antibacterial and cytotoxic effect of Myrciaria dubia (Camu camu) (M. dubia) methanol extract, against Streptococcus mutans (ATCC 25175) (S. mutans) and Streptococcus sanguinis (ATCC 10556) (S. sanguinis). Methods: Two methanol extracts of M. dubia were prepared in vitro, from the seeds and pulp. Ten independent tests were prepared for each type of extract, using 0.12% chlor-hexidine solution as positive control. Agar diffusion test was used by preparing wells with the experimental solutions cultivated in anaerobic conditions for 48 h at 37 ° C. Mean-while, the minimum inhibitory concentration and the cytotoxic effect over MDCK cell line was found. Results: A higher antibacterial effect was observed with the methanol seed extract with an inhibitory halo of (21.36 ± 6.35) mm and (19.21 ± 5.18) mm against S. mutans and S. sanguinis, respectively. The methanol extract of the pulp had an effect of (16.20 ± 2.08) mm and (19.34 ± 2.90) mm, respectively. The minimum inhibitory concentration of the pulp extract was 62.5μg/mL for both strains, whereas for the seed antibacterial activity was observed even at low concentrations. The CC50 of the seeds extract was at a higher con-centration than 800μg/mL and 524.37μg/mL for the pulp extract. Conclusions: The experimental findings demonstrated the antibacterial effect of the methanol extract of M. dubia against S. mutans and S. sanguinis. These extracts were not cytotoxic at high concentrations.

Sphagnum mosses as a microhabitat for invertebrates in acidified lakes and the colour adaptation and substrate preference in Leucorrhinia dubia (Odonata, Anisoptera)

Energy Technology Data Exchange (ETDEWEB)

Henrikson, B.-I. (Dept. of Zoology, Sect. of Animal Ecology, Univ. of Goeteborg, Goeteborg (Sweden))

1993-01-01

The increase of peat mosses, Sphagnum spp., in acidified lakes leads to a changed microhabitat structure for benthic invertebrates. The importance of this change was investigated for some benthic invertebrates. Comparisons between quantitative samples of Sphagnum and debris within the acidified Lake Stora Haestevatten, in the Lake Gaardsjoen catchment of SW Sweden, showed significantly higher abundances of Chironomidae, Ceratopogonidae, Odonata, Trichoptera, Cladocera and Argyroneta aquatica (Araneae) in Sphagnum. For chironomidae and Cladocera the differences were tenfold. Special reference was made to the libellulid Leucorrhinia dubia which is common in acid lakes. In a laboratory test, late instar larvae of L. dubia were shown to change colour to correspond to the brown and green colour of Sphagnum. This result was completed with a field test where larvae of L. dubia were significantly more common in Sphagnum of the same colour as the larvae. The ability to change colour may have an adaptive value when coexisting with visual predators. Small larvae were more prevalent in Sphagnum and they also showed a preference for this substrate in the laboratory test. Laboratory tests showed mediumsized larvae preferred Sphagnum. Larvae of L. dubia were more successful as predators on Asellus aquaticus in Sphagnum substrate than in debris in the laboratory test. Laboratory predation tests with notonecta glauca, Corixa dentipes, Acilius sulcatus, Hyphydrus ovatus and L. dubia showed that they could all feed on larvae of L. dubia. The complex habitat structure of Sphagnum is probably the reason for the high abundance of invertebrates since it may serve as both shelter against predation and as foraging sites. it is probably important as a key habitat for young instars of, for example, L. dubia. In lakes with large Sphagnum mats, L. dubia can coexist with fish. The expansion of Sphagnum due to acidification will probably benefit many acid-tolerant invertebrate species. (au)

Large test rigs verify Clinch River control rod reliability

International Nuclear Information System (INIS)

Michael, H.D.; Smith, G.G.

1983-01-01

The purpose of the Clinch River control test programme was to use multiple full-scale prototypic control rod systems for verifying the system's ability to perform reliably during simulated reactor power control and emergency shutdown operations. Two major facilities, the Shutdown Control Rod and Maintenance (Scram) facility and the Dynamic and Seismic Test (Dast) facility, were constructed. The test programme of each facility is described. (UK)

Radionuclide accumulations in Clinch River fish

International Nuclear Information System (INIS)

Oakes, T.W.; Easterly, C.E.; Shank, K.E.

1976-01-01

Fish samples were collected from several locations above Melton Hill Dam, which is upstream from the liquid effluent release point of the Oak Ridge National Laboratory. The sampling locations were chosen to determine the accumulation of natural and man-made radionuclides in fish from areas in the Clinch River not influenced by the Laboratory's liquid effluents. Bass, carp, crappie, shad, bluegill, and other sunfish were collected; ten fish per species were composited to form a single sample for each location. The gamma-emitting radionuclide concentrations were determined by gamma-ray spectroscopy. Estimates of radiological dose to man subsequent to ingestion of these fish are made

Occurrence, pathology, and ultrastructure of iridovirus and cytoplasmic polyhedrosis viruses in daphnids from the Czech Republic

Czech Academy of Sciences Publication Activity Database

Vávra, Jiří; Bílý, Tomáš; Nebesářová, Jana; Federici, B. A.

2016-01-01

Roč. 140, Jul 19 (2016), s. 35-38 ISSN 0022-2011 R&D Projects: GA MŠk LM2015062; GA TA ČR(CZ) TE01020118 Institutional support: RVO:60077344 Keywords : iridovirus * cytoplasmic polhedrosis virus * Crustacean * Daphnid * geographical occurrence * Czech Republic * AF microscopy * electron tomography Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.379, year: 2016

Clinch River Breeder Reactor Plant: a building block in nuclear technology

International Nuclear Information System (INIS)

McCormack, M.

1979-01-01

Interest in breeder reactors dates from the Manhatten Project to the present effort to build the Clinch River Liquid Metal Fast Breeder Reactor (LMFBR) demonstration plant. Seven breeder-type reactors which were built during this time are described and their technological progress assessed. The Clinch River Breeder Reactor Project (CRBRP) has been designed to demonstrate that it can be licensed, can operate on a large power grid, and can provide industry with important experience. As the next logical step in LMFBR development, the project has suffered repeated cancellation efforts with only minor modifications to its schedule. Controversies have developed over the timing of a large-scale demonstration plant, the risks of proliferation, economics, and other problems. Among the innovative developments adopted for the CRBRP is a higher thermal efficiency potential, the type of development which Senator McCormack feels justifies continuing the project. He argues that the nuclear power program can and should be revitalized by continuing the CRBRP

Clinch River Breeder Reactor Plant: a building block in nuclear technology

Energy Technology Data Exchange (ETDEWEB)

McCormack, M.

1979-01-01

Interest in breeder reactors dates from the Manhatten Project to the present effort to build the Clinch River Liquid Metal Fast Breeder Reactor (LMFBR) demonstration plant. Seven breeder-type reactors which were built during this time are described and their technological progress assessed. The Clinch River Breeder Reactor Project (CRBRP) has been designed to demonstrate that it can be licensed, can operate on a large power grid, and can provide industry with important experience. As the next logical step in LMFBR development, the project has suffered repeated cancellation efforts with only minor modifications to its schedule. Controversies have developed over the timing of a large-scale demonstration plant, the risks of proliferation, economics, and other problems. Among the innovative developments adopted for the CRBRP is a higher thermal efficiency potential, the type of development which Senator McCormack feels justifies continuing the project. He argues that the nuclear power program can and should be revitalized by continuing the CRBRP.

An Experimental Study on Micro Clinching of Metal Foils with Cutting by Laser Shock Forming.

Science.gov (United States)

Wang, Xiao; Li, Cong; Ma, Youjuan; Shen, Zongbao; Sun, Xianqing; Sha, Chaofei; Gao, Shuai; Li, Liyin; Liu, Huixia

2016-07-13

This paper describes a novel technique for joining similar and dissimilar metal foils, namely micro clinching with cutting by laser shock forming. A series of experiments were conducted to study the deformation behavior of single layer material, during which many important process parameters were determined. The process window of the 1060 pure aluminum foils and annealed copper foils produced by micro clinching with cutting was analyzed. Moreover, similar material combination (annealed copper foils) and dissimilar material combination (1060 pure aluminum foils and 304 stainless steel foils) were successfully achieved. The effect of laser energy on the interlock and minimum thickness of upper foils was investigated. In addition, the mechanical strength of different material combinations joined by micro clinching with cutting was measured in single lap shearing tests. According to the achieved results, this novel technique is more suitable for material combinations where the upper foil is thicker than lower foil. With the increase of laser energy, the interlock increased while the minimum thickness of upper foil decreased gradually. The shear strength of 1060 pure aluminum foils and 304 stainless steel foils combination was three times as large as that of 1060 pure aluminum foils and annealed copper foils combination.

Transport and accumulation of cesium-137 and mercury in the Clinch River and Watts Bar Reservoir system

International Nuclear Information System (INIS)

Olsen, C.R.; Larsen, I.L.; Lowry, P.D.; Moriones, C.R.; Ford, C.J.; Dearstone, K.C.; Turner, R.R.; Kimmel, B.L.; Brandt, C.C.

1992-06-01

Operations and waste disposal activities at the Oak Ridge Y-12 Plant, the Oak Ridge National Laboratory (ORNL), and the Oak Ridge K-25 Site (formerly the Oak Ridge Gaseous Diffusion Plant) on the US Department of Energy (DOE) Oak Ridge Reservation (ORR) have introduced a variety of airborne, liquid, and solid wastes into the surrounding environment. Some of these wastes may affect off-site areas by entering local streams, which ultimately drain into the Clinch and Tennessee river system. Previously reported concentrations of radionuclides, metals and organic compounds in water, sediment, and biota of the Clinch River and Watts Bar Reservoir suggest the presence of a variety of contaminants of possible concern to the protection of human health and the environment. The work reported here represents part of the initial scoping phase for the Clinch River RCRA Facility Investigation. In this work, the distribution of 137 Cs is used to identify contaminant accumulation patterns and potential problem, or ''hot-spot,'' areas with regard to environmental hazard or human health. Radiocesium was chosen for this scoping effort because (1) its history of release into the Clinch River is reasonably well documented, (2) it is easy and inexpensive to measure by gamma spectrometry, and (3) it is rapidly sorbed to particulate matter and thus serves as a cost-effective tracer for identifying the transport and accumulation patterns of many other particle-reactive contaminants, such as mercury (Hg), lead (Pb), and plutonium (Pu), and polychlorinated biphenyls (PCBs)

Acute toxicity screening of Hanford Site waste grouts using aquatic invertebrates

International Nuclear Information System (INIS)

Rebagay, T.V.; Dodd, D.A.; Lockrem, L.L.; Voogd, J.A.

1993-01-01

Liquid wastes containing radioactive, hazardous, and regulated chemicals have been generated throughout the 50 years operation of the Hanford Site of the US Department of Energy near Richland, Washington. The current strategy for the disposal of the low-level radioactive portion of these wastes involves immobilization of the waste in the form of grout. Because the potential risk of animal and plant exposure to grouts is unknown, acute toxicity screening of grouts is needed. Grouts were prepared by mixing a surrogate nonradioactive liquid waste with a blend consisting of cement, fly ash, and clay. Aqueous extracts of the grout were then screened for acute toxicity using aquatic invertebrates as test organisms and a fluorogenic substrate as the toxic stress indicator. After a 1-hour exposure of juvenile daphnids (D, magna, D. pulex, and C. dubia) to the grout extracts followed by a 15-minute reaction with the fluorogenic substrate, the degree of in vivo enzymatic inhibition was measured by the number of resulting fluorescent daphnids. The EC50 values calculated by probit analysis were 2,877 mg/L, 2,983 mg/L, and 3,174 mg/L for D. pulex, D. magna, and C. dubia, respectively. The slight difference in the responses may be attributed to the subjective pass-fail scoring of the fluorescence criterion. The results indicated that the grout studied is nonhazardous and nondangerous

Myrciaria dubia, an Amazonian fruit: population structure and its implications for germplasm conservation and genetic improvement.

Science.gov (United States)

Nunes, C F; Setotaw, T A; Pasqual, M; Chagas, E A; Santos, E G; Santos, D N; Lima, C G B; Cançado, G M A

2017-03-22

Myrciaria dubia (camu-camu) is an Amazon tree that produces a tart fruit with high vitamin C content. It is probably the fruit with the highest vitamin C content among all Brazilian fruit crops and it can be used to supplement daily vitamin C dose. This property has attracted the attention of consumers and, consequently, encouraged fruit farmers to produce it. In order to identify and select potential accessions for commercial exploitation and breeding programs, M. dubia has received considerable research attention. The identification and characterization of genetic diversity, as well as identification of the population structure of accessions preserved in germplasm banks are fundamental for the success of any breeding program. The objective of this study was to evaluate the genetic variability of 10 M. dubia populations obtained from the shores of Reis Lake, located in the municipality of Caracaraí, Roraima, Brazil. Fourteen polymorphic inter simple sequence repeat (ISSR) markers were used to study the population genetic diversity, which resulted in 108 identified alleles. Among the 14 primers, GCV, UBC810, and UBC827 produced the highest number of alleles. The study illustrated the suitability and efficiency of ISSR markers to study the genetic diversity of M. dubia accessions. We also revealed the existence of high genetic variability among both accessions and populations that can be exploited in future breeding programs and conservation activities of this species.

An Experimental Study on Micro Clinching of Metal Foils with Cutting by Laser Shock Forming

Directory of Open Access Journals (Sweden)

Xiao Wang

2016-07-01

Full Text Available This paper describes a novel technique for joining similar and dissimilar metal foils, namely micro clinching with cutting by laser shock forming. A series of experiments were conducted to study the deformation behavior of single layer material, during which many important process parameters were determined. The process window of the 1060 pure aluminum foils and annealed copper foils produced by micro clinching with cutting was analyzed. Moreover, similar material combination (annealed copper foils and dissimilar material combination (1060 pure aluminum foils and 304 stainless steel foils were successfully achieved. The effect of laser energy on the interlock and minimum thickness of upper foils was investigated. In addition, the mechanical strength of different material combinations joined by micro clinching with cutting was measured in single lap shearing tests. According to the achieved results, this novel technique is more suitable for material combinations where the upper foil is thicker than lower foil. With the increase of laser energy, the interlock increased while the minimum thickness of upper foil decreased gradually. The shear strength of 1060 pure aluminum foils and 304 stainless steel foils combination was three times as large as that of 1060 pure aluminum foils and annealed copper foils combination.

Myrciaria dubia (Kunth Mc Vaugh "Camu Camu"

Directory of Open Access Journals (Sweden)

Ana M. Córdova

2014-01-01

Full Text Available Debido a la alta variabilidad en la producción de vitamina C enMyrciariadubia"camucamu", es necesarioestablecer procedimientos biotecnológicos para la propagación clonal masiva de genotipos promisorios de estaespecie.El objetivo fue establecer un método eficiente para inducir la formación de callosin vitroa partir deexplantesdeM. dubia. Los explantes de hojas y nudos se obtuvieron de ramas cultivadas en el laboratorio y lapulpa a partir defrutos colectados en el campo. Estosfueron desinfectados y sembrados en medio Murashige-Skoog (1962 suplementado con ácido 2,4-diclorofenoxiacético (2,4-D, bencilaminopurina (BAP y kinetina(Kin. Los cultivos fueron mantenidos a 25±2°C, en oscuridad por 2 semanas y posteriormente con unfotoperiodo de 16 horas luz y 8 horas de oscuridad por 6 semanas. El tratamiento con 2 mg/L de 2,4-D y 0,1mg/L de BAP estimuló mayor callogénesis en los tres tipos de explantes. Los callos se generaron a partir de laprimera semana (nudos, cuarta semana (hojas y sexta semana (pulpa y estos fueron friables (hojas y nudosy no friables (pulpa. En conclusión, el método descrito es eficiente para inducir callosin vitroen hojas, nudosy pulpa deM. dubia, siendo los explantes de hojas y nudos los más idóneos para la obtención de callos.

Clinch River Environmental Restoration Program

International Nuclear Information System (INIS)

Cook, R.B.

1992-01-01

This report consists of tables and listings from the results of the Phase I data gathering activities of the Clinch River Environmental Restoration Program (CR-ERP). The table of contents outlines the presentation of the material and has been annotated to indicate the key fields used to order the printing of each data table. Definitions of selected column headings are provided. Sample collection information is shown first and then more specific information for each matrix type is presented. The analytical results have been reviewed by independent validators and the qualifiers shown are the results of their efforts. No data that were rejected by the validation process are included in this listing. Only results of routine samples are listed; quality control sample results were excluded. All data, both detected and nondetected values, were used to calculated the summary table values. However, only Detected values are given on the analyte specific listings

Antibacterial activity of Myrciaria dubia (Camu camu against Streptococcus mutans and Streptococcus sanguinis

Directory of Open Access Journals (Sweden)

Rosella Camere-Colarossi

2016-09-01

Conclusions: The experimental findings demonstrated the antibacterial effect of the methanol extract of M. dubia against S. mutans and S. sanguinis. These extracts were not cytotoxic at high concentrations.

Remedial investigation/feasibility study for the Clinch River/Poplar Creek operable unit. Volume 4. Appendix F

International Nuclear Information System (INIS)

1995-09-01

This section contains ecotoxicological profiles for the COPECs for the combined Remedial Investigation/Feasibility Study Report for the Clinch River/Poplar Crack (CR/PC) Operable Unit (OU). The ecotoxicological information is presented for only those endpoints for which the chemicals are COPECs. The CR/PC OU is located in Anderson and Roane Counties, Tennessee and consists of the Clinch River and several of its embayments in Melton Hill and Watts Bar Reservoirs. These waters have received hazardous substances released over a period of 50 years from the US Department of Energy's Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act. A remedial investigation has been conducted to determine the current nature and extent of any contamination and to assess the resulting risk to human health and the environment. The feasibility study evaluates remedial action alternatives to identify any that are feasible for implementation and that would effectively reduce risk. Historical studies had indicated that current problems would likely include 137 Cs in sediment of the Clinch River, mercury in sediment and fish of Poplar Creek and PCBs and pesticides in fish from throughout the OU. Peak releases of mercury and 137 Cs occurred over 35 years ago, and current releases are low. Past releases of PCBs from the ORR are poorly quantified, and current releases are difficult to quantify because levels are so low. The site characterization focused on contaminants in surface water, sediment, and biota. Contaminants in surface water were all found to be below Ambient Water Quality Criteria. Other findings included the following: elevated metals including cesium 137 and mercury in McCoy Branch sediments; PCBs and chlordane elevated in several fish species, presenting the only major human health risk, significant ecological risks in Poplar Creek but not in the Clinch River

Remedial investigation/feasibility study for the Clinch River/Poplar Creek operable unit. Volume 4. Appendix F

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-09-01

This section contains ecotoxicological profiles for the COPECs for the combined Remedial Investigation/Feasibility Study Report for the Clinch River/Poplar Crack (CR/PC) Operable Unit (OU). The ecotoxicological information is presented for only those endpoints for which the chemicals are COPECs. The CR/PC OU is located in Anderson and Roane Counties, Tennessee and consists of the Clinch River and several of its embayments in Melton Hill and Watts Bar Reservoirs. These waters have received hazardous substances released over a period of 50 years from the US Department of Energy`s Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act. A remedial investigation has been conducted to determine the current nature and extent of any contamination and to assess the resulting risk to human health and the environment. The feasibility study evaluates remedial action alternatives to identify any that are feasible for implementation and that would effectively reduce risk. Historical studies had indicated that current problems would likely include {sup 137}Cs in sediment of the Clinch River, mercury in sediment and fish of Poplar Creek and PCBs and pesticides in fish from throughout the OU. Peak releases of mercury and {sup 137}Cs occurred over 35 years ago, and current releases are low. Past releases of PCBs from the ORR are poorly quantified, and current releases are difficult to quantify because levels are so low. The site characterization focused on contaminants in surface water, sediment, and biota. Contaminants in surface water were all found to be below Ambient Water Quality Criteria. Other findings included the following: elevated metals including cesium 137 and mercury in McCoy Branch sediments; PCBs and chlordane elevated in several fish species, presenting the only major human health risk, significant ecological risks in Poplar Creek but not in the Clinch River.

Remedial investigation/feasibility study for the Clinch River/Poplar Creek operable unit. Volume 3. Appendix E

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-09-01

This document contains Appendix E: Toxicity Information and Uncertainty Analysis, description of methods, from the combined Remedial Investigation/Feasibility Study Report for the Clinch River/Poplar Crack (CR/PC) Operable Unit (OU). The CR/PC OU is located in Anderson and Roane Counties, Tennessee and consists of the Clinch River and several of its embayments in Melton Hill and Watts Bar Reservoirs. These waters have received hazardous substances released over a period of 50 years from the US Department of Energy`s Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act. A remedial investigation has been conducted to determine the current nature and extent of any contamination and to assess the resulting risk to human health and the environment. The feasibility study evaluates remedial action alternatives to identify any that are feasible for implementation and that would effectively reduce risk. Historical studies had indicated that current problems would likely include {sup 137}Cs in sediment of the Clinch River, mercury in sediment and fish of Poplar Creek and PCBs and pesticides in fish from throughout the OU. Peak releases of mercury and {sup 137}Cs occurred over 35 years ago, and current releases are low. Past releases of PCBs from the ORR are poorly quantified, and current releases are difficult to quantify because levels are so low. The site characterization focused on contaminants in surface water, sediment, and biota. Contaminants in surface water were all found to be below Ambient Water Quality Criteria. Other findings included the following: elevated metals including cesium 137 and mercury in McCoy Branch sediments; PCBs and chlordane elevated in several fish species, presenting the only major human health risk, significant ecological risks in Poplar Creek but not in the Clinch River.

Remedial investigation/feasibility study for the Clinch River/Poplar Creek operable unit. Volume 1. Main text

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-09-01

This is the combined Remedial Investigation/Feasibility Study Report for the Clinch River/Poplar Crack (CR/PC) Operable Unit (OU). The CR/PC OU is located in Anderson and Roane Counties, Tennessee and consists of the Clinch River and several of its embayments in Melton Hill and Watts Bar Reservoirs. These waters have received hazardous substances released over a period of 50 years from the US Department of Energy`s Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act. A remedial investigation has been conducted to determine the current nature and extent of any contamination and to assess the resulting risk to human health and the environment. The feasibility study evaluates remedial action alternatives to identify any that are feasible for implementation and that would effectively reduce risk. Historical studies had indicated that current problems would likely include {sup 137}Cs in sediment of the Clinch River, mercury in sediment and fish of Poplar Creek and PCBs and pesticides in fish from throughout the OU. Peak releases of mercury and {sup 137}Cs occurred over 35 years ago, and current releases are low. Past releases of PCBs from the ORR are poorly quantified, and current releases are difficult to quantify because levels are so low. The site characterization focused on contaminants in surface water, sediment, and biota. Contaminants in surface water were all found to be below Ambient Water Quality Criteria. Other findings included the following: elevated metals including cesium 137 and mercury in McCoy Branch sediments; PCBs and chlordane elevated in several fish species, presenting the only major human health risk, significant ecological risks in Poplar Creek but not in the Clinch River.

Remedial investigation/feasibility study for the Clinch River/Poplar Creek operable unit. Volume 3. Appendix E

International Nuclear Information System (INIS)

1995-09-01

This document contains Appendix E: Toxicity Information and Uncertainty Analysis, description of methods, from the combined Remedial Investigation/Feasibility Study Report for the Clinch River/Poplar Crack (CR/PC) Operable Unit (OU). The CR/PC OU is located in Anderson and Roane Counties, Tennessee and consists of the Clinch River and several of its embayments in Melton Hill and Watts Bar Reservoirs. These waters have received hazardous substances released over a period of 50 years from the US Department of Energy's Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act. A remedial investigation has been conducted to determine the current nature and extent of any contamination and to assess the resulting risk to human health and the environment. The feasibility study evaluates remedial action alternatives to identify any that are feasible for implementation and that would effectively reduce risk. Historical studies had indicated that current problems would likely include 137 Cs in sediment of the Clinch River, mercury in sediment and fish of Poplar Creek and PCBs and pesticides in fish from throughout the OU. Peak releases of mercury and 137 Cs occurred over 35 years ago, and current releases are low. Past releases of PCBs from the ORR are poorly quantified, and current releases are difficult to quantify because levels are so low. The site characterization focused on contaminants in surface water, sediment, and biota. Contaminants in surface water were all found to be below Ambient Water Quality Criteria. Other findings included the following: elevated metals including cesium 137 and mercury in McCoy Branch sediments; PCBs and chlordane elevated in several fish species, presenting the only major human health risk, significant ecological risks in Poplar Creek but not in the Clinch River

Remedial investigation/feasibility study for the Clinch River/Poplar Creek operable unit. Volume 1. Main text

International Nuclear Information System (INIS)

1995-09-01

This is the combined Remedial Investigation/Feasibility Study Report for the Clinch River/Poplar Crack (CR/PC) Operable Unit (OU). The CR/PC OU is located in Anderson and Roane Counties, Tennessee and consists of the Clinch River and several of its embayments in Melton Hill and Watts Bar Reservoirs. These waters have received hazardous substances released over a period of 50 years from the US Department of Energy's Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act. A remedial investigation has been conducted to determine the current nature and extent of any contamination and to assess the resulting risk to human health and the environment. The feasibility study evaluates remedial action alternatives to identify any that are feasible for implementation and that would effectively reduce risk. Historical studies had indicated that current problems would likely include 137 Cs in sediment of the Clinch River, mercury in sediment and fish of Poplar Creek and PCBs and pesticides in fish from throughout the OU. Peak releases of mercury and 137 Cs occurred over 35 years ago, and current releases are low. Past releases of PCBs from the ORR are poorly quantified, and current releases are difficult to quantify because levels are so low. The site characterization focused on contaminants in surface water, sediment, and biota. Contaminants in surface water were all found to be below Ambient Water Quality Criteria. Other findings included the following: elevated metals including cesium 137 and mercury in McCoy Branch sediments; PCBs and chlordane elevated in several fish species, presenting the only major human health risk, significant ecological risks in Poplar Creek but not in the Clinch River

Preconstruction radioactivity levels in the vicinity of the proposed Clinch River Breeder Reactor Project

International Nuclear Information System (INIS)

1984-05-01

Routine samples of ground water, river water, and bottom sediment were collected from the Clinch River in 1983 in the preconstruction-construction phase of the CRBRP environmental radiological monitoring program. The water samples analyzed for iodine-131 yielded only a slight indication of the presence of I-131 at levels below the nominal lower limit of detection of 0.5 pCi/L. The only significant radioisotopes identified in sediment samples were 137 Cs, 60 Co, and the naturally occurring 40 K. The results for 137 Cs vary from 2.2 to 10.1 pCi/g (dry weight), while the results for 60 Co range from 0.35 to 1.2 pCi/g (dry weight). With the exception of tritium, no significant radioactivity was detected in ground or surface water at the CRBRP site. Tritium concentrations ranging from 12,667 to 12,823 pCi/L were found in samples of surface water taken from the Clinch River below Melton Hill Dam while samples taken at the dam exhibited tritium levels from 28 to 942 pCi/L. These elevated tritium levels in the Clinch River below Melton Hill Dam are attributable to DOE operations at Oak Ridge. The external gamma radiation levels measured at the CRBRP site averaged 17.4 +- 3.2 mR/quarter for 1983. This is consistent with levels measured at TVA's nonoperating nuclear power plant construction sites. 3 figures, 8 tables

Ecotoxicological evaluation of water of the hydrographic Basin of the Una River using the bioindicator Ceriodaphnia dubia

Directory of Open Access Journals (Sweden)

Tatiane Alves

2013-12-01

Full Text Available The majority of the Una River Basin is located in Taubaté County and contributes significantly to its water supply. The main goal of this research was to evaluate the water quality of the Una River using the microcrustacean C. dubia as bioindicator for tests of chronic and acute toxicity. Bimonthly water samples were obtained from each of six localities throughout the Una Basin, from March to October, 2011. Physical-chemical water parameters such as pH, electrical conductivity, hardness, dissolved oxygen and precipitation were measured and correlated to the C. dubia reproductive rates. No significant relationships were found between the water’s electrical conductivity and precipitation with respect to bioindicator reproductive rates. However, at the Sete Voltas, Antas and Rocinha Sub-Basins, significant interactions were detected between some water parameters and reproductive rates, suggesting that water may constrain the reproduction of C. dubia. Acute toxicity was not detected in any of the six sites, while chronic toxicity was recorded at Rocinha, Sete Voltas, Antas, Médio and Baixo Una Sub-Basins. In general, the water quality of the Una Basin, as indicated by the absence of acute toxicity, still remains in an acceptable conservation condition. Caution is needed, however, since slight pollution sources are causing chronic toxicity in some localities. In addition, as the microcrustacean C. dubia, appeared to be a reliable bioindicator in this investigation, we suggest that it be used for continuous water quality monitoring programs.

Ceriodaphnia dubia as a potential bio-indicator for assessing acute aluminum oxide nanoparticle toxicity in fresh water environment.

Directory of Open Access Journals (Sweden)

Sunandan Pakrashi

Full Text Available Growing nanomaterials based consumer applications have raised concerns about their potential release into the aquatic ecosystems and the consequent toxicological impacts. So environmental monitoring of the nanomaterials in aqueous systems becomes imperative. The current study reveals the potential of Ceriodaphnia dubia (C. dubia as a bio-indicator for aluminum oxide nanoparticles in a fresh water aquatic ecosystem where it occupies an important ecological niche as a primary consumer. This study aims to investigate the aluminium oxide nanoparticle induced acute toxicity on Ceriodaphnia dubia in a freshwater system. The bioavailability of the aluminum oxide nanoparticles has been studied with respect to their aggregation behavior in the system and correlated with the toxicity endpoints. The oxidative stress generated by the particles contributed greatly toward their toxicity. The crucial role of leached aluminium ion mediated toxicity in the later phases (48 h and 72 h in conjunction with the effects from the nano-sized particles in the initial phases (24 h puts forth the dynamics of nanotoxicity in the test system. The internalization of nanoparticles (both gross and systemic uptake as substantiated through the transmission electron microscopy (TEM and inductively coupled plasma optical emission spectral (ICP-OES analysis was another major contributor toward acute toxicity. Concluding the present study, Ceriodaphnia dubia can be a promising candidate for bio-monitoring the aluminium oxide nanoparticles in a fresh water system.

Data summary for the near-shore sediment characterization task of the Clinch River Environmental Restoration Program

International Nuclear Information System (INIS)

Levine, D.A.; Hargrove, W.W.; Campbell, K.R.; Wood, M.A.; Rash, C.D.

1994-10-01

This report presents the results of the Near-Shore Sediment Characterization Task of the Clinch River Environmental Restoration Program (CR-ERP). The goals of the task were to (1) determine the extent to which near-shore surface sediments are contaminated by releases from the Oak Ridge Reservation (ORR) and (2) provide data for the Watts Bar Reservoir Interagency Permitting Group (WBRIPG) to evaluate the human health risks from exposure to sediments during and following dredging operations. The data collected for this task are also to be used in the Remedial Investigation/Feasibility Study (RLTS) for the CR-ERP operable units (Lower Watts Bar and Clinch River) to characterize the human health risk associated with exposure to near-shore sediments throughout the Watts Bar Reservoir

Clinch River Breeder Reactor secondary control rod system

International Nuclear Information System (INIS)

McKeehan, E.R.; Sim, R.G.

1977-01-01

The shutdown system for the Clinch River Breeder Reactor (CRBR) includes two independent systems--a primary and a secondary system. The Secondary Control Rod System (SCRS) is a new design which is being developed by General Electric to be independent from the primary system in order to improve overall shutdown reliability by eliminating potential common-mode failures. The paper describes the status of the SCRS design and fabrication and testing activities. Design verification testing on the component level is largely complete. These component tests are covered with emphasis on design impact results. A prototype unit has been manufactured and system level tests in sodium have been initiated

Safety-Evaluation Report related to the construction of the Clinch River Breeder Reactor Plant. Docket No. 50-537

International Nuclear Information System (INIS)

1983-03-01

The Safety-Evaluation Report for the application by the United States Department of Energy, Tennessee Valley Authority, and the Project Management Corporation, as applicants and owners, for a license to construct the Clinch River Breeder Reactor Plant (docket No. 50-537) has been prepared by the Office of Nuclear Reactor Regulation of the United States Nuclear Regulatory Commission. The facility will be located on the Clinch River approximately 12 miles southwest of downtown Oak Ridge and 25 miles west of Knoxville, Tennessee. Subject to resolution of the items discussed in this report, the staff concludes that the construction permit requested by the applicants should be issued

Impact of Microplastic Beads and Fibers on Waterflea (Ceriodaphnia dubia) Survival, Growth, and Reproduction: Implications of Single and Mixture Exposures.

Science.gov (United States)

Ziajahromi, Shima; Kumar, Anupama; Neale, Peta A; Leusch, Frederic D L

2017-11-21

There is limited knowledge regarding the adverse effects of wastewater-derived microplastics, particularly fibers, on aquatic biota. In this study, we examined the acute (48 h) and chronic (8 d) effects of microplastic polyester fibers and polyethylene (PE) beads on freshwater zooplankton Ceriodaphnia dubia. We also assessed the acute response of C. dubia to a binary mixture of microplastic beads and fibers for the first time. Acute exposure to fibers and PE beads both showed a dose-dependent effect on survival. An equitoxic binary mixture of beads and fibers resulted in a toxic unit of 1.85 indicating less than additive effects. Chronic exposure to lower concentrations did not significantly affect survival of C. dubia, but a dose-dependent effect on growth and reproduction was observed. Fibers showed greater adverse effects than PE beads. While ingestion of fibers was not observed, scanning electron microscopy showed carapace and antenna deformities after exposure to fibers, with no deformities observed after exposure to PE beads. While much of the current research has focused on microplastic beads, our study shows that microplastic fibers pose a greater risk to C. dubia, with reduced reproductive output observed at concentrations within an order of magnitude of reported environmental levels.

Fumigant and repellent activities of essential oil extracted from Artemisia dubia and its main compounds against two stored product pests.

Science.gov (United States)

Liang, Jun-Yu; Guo, Shan-Shan; Zhang, Wen-Juan; Geng, Zhu-Feng; Deng, Zhi-Wei; Du, Shu-Shan; Zhang, Ji

2018-05-01

The major chemical constituents of the essential oil extracted from Artemisia dubia wall. ex Bess. (Family: Asteraceae) were found as terpinolene (19.02%), limonene (17.40%), 2,5-etheno[4.2.2]propella-3,7,9-triene (11.29%), isoelemicin (11.05%) and p-cymene-8-ol (5.93%). Terpinolene and limonene were separated as main components from the essential oil. The essential oil showed fumigant toxicity against Tribolium castaneum and Liposcelis bostrychophila with LC 50 values of 49.54 and 0.74 mg/L, respectively. The essential oil and isolated compounds of A. dubia showed repellency activities against both insects. Terpinolene and limonene showed the fumigant toxicity against T. castaneum. Terpinolene showed obvious fumigant toxicity against L. bostrychophila. The results indicated that the essential oil of A. dubia had potential to be developed into natural insecticides for controlling stored product pests.

CLONACIÓN Y FILOGENIA MOLECULAR DE UN SEGMENTO DEL GEN CODANTE DE LA ACTINA DE MYRCIARIA DUBIA “CAMU-CAMU”: UN CANDIDATO PARA GEN DE REFERENCIA

Directory of Open Access Journals (Sweden)

Juan Carlos Castro Gómez

2012-12-01

Full Text Available Myrciaria dubia “camu-camu” es un frutal amazónico caracterizado por su amplia variación de vitamina C. Pero los estudios genético moleculares que puedan explicar esta variación son limitados. Por ello nuestro objetivo fue realizar la clonación y filogenia molecular de un segmento del gen codante de la actina de M. dubia. Las muestras fueron obtenidas de la colección de germoplasma del INIA. Luego, el ARN fue purificado y mediante RT-PCR con cebadores degenerados se amplificó un segmento del gen. En base a la secuencia obtenida se diseñaron cebadores específicos para PCR en tiempo real. Los resultados muestran que se ha aislado, clonado y secuenciado un segmento del gen codante de actina de M. dubia y detectado su expresión en hojas, pulpa y cáscara de M. dubia. Así, con el soporte de herramientas bioinformáticas y uso de técnicas de biología molecular hemos aislado, clonado y secuenciado un segmento del gen codante de la actina de M. dubia. Asimismo, los análisis realizados muestran que el gen se expresa y presenta niveles similares de expresión en hojas, pulpa y cáscara de M. dubia. Sin embargo, es necesario realizar más experimentos a fin de verificar su estabilidad de expresión.

Toxicity of noradrenaline, a novel anti-biofouling component, to two non-target zooplankton species, Daphnia magna and Ceriodaphnia dubia.

Science.gov (United States)

Overturf, C L; Wormington, A M; Blythe, K N; Gohad, N V; Mount, A S; Roberts, A P

2015-05-01

Noradrenaline (NA) is the active component of novel antifouling agents and acts by preventing attachment of fouling organisms. The goal of this study was to examine the toxicity of NA to the non-target zooplankton D. magna and C. dubia. Neonates were exposed to one of five concentrations of NA and effects on survival, reproduction and molting were determined. Calculated LC50 values were determined to be 46 and 38 μM in C. dubia and D. magna, respectively. A 10-day C. dubia study found that reproduction metrics were significantly impacted at non-lethal concentrations. In D. magna, concentrations greater than 40 μM significantly impacted molting. A toxicity test was conducted with D. magna using oxidized NA, which yielded similar results. These data indicate that both NA and oxidized NA are toxic to non-target zooplankton. Results obtained from this study can be used to guide future ecological risk assessments of catecholamine-based antifouling agents. Copyright © 2015. Published by Elsevier Inc.

Human factors engineering in Clinch River Breeder plant design

International Nuclear Information System (INIS)

Planchon, H.P. Jr.; Kaushal, N.N.; Snider, J.

1982-01-01

The Clinch River Breeder Reactor Plant (CRBRP) Project formed a Control Room Task Force to ensure that lessons learned from the Three Mile Island accident are incorporated into the design. The charter for the Control Room Task Force was to review plant operations from the control room. The focus was on the man-machine interface to ensure that the systems' designs and operator actions meshed to properly support plant operation during normal and off-normal conditions. Specific items included for review are described. This paper describes the methodology utilized to accomplish the Task Forces' objectives and the results of the review

An efficient method for in vitro callus induction in Myrciaria dubia (Kunth Mc Vaugh "Camu Camu"

Directory of Open Access Journals (Sweden)

Ana M. Córdova

2014-03-01

Full Text Available Due to the high variability in vitamin C production in Myrciaria dubia "camu camu", biotechnological procedures are necessary for mass clonal propagation of promising genotypes of this species. The aim was to establish an efficient method for in vitro callus induction from explants of M. dubia. Leaf and knot sex plants were obtained from branches grown in the laboratory and from fruit pulp collected in the field. These were desinfected and sown on Murashige-Skoog (1962 medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D, benzylaminopurine (BAP and kinetin(Kin. The cultures were maintained at 25±2°C in darkness for 2 weeks and subsequently with a photoperiod of 16 hours in light and 8 hours in dark for 6 weeks. Treatment with 2 mg/L 2,4-D and 0.1 mg/L BAP allowed major callus formation in the three types of explants. Calluswere generated from the first week (knots, fourth week (leaves and sixth week (pulp and these were friable (leaves and nodes and non-friable (pulp. In conclusion, the described method is efficient for in vitro callus induction in leaves, knots and pulp of M. dubia, been leaves and knots explants more suitable for callus obtention

Remedial investigation/feasibility study for the Clinch River/Poplar Creek operable unit. Volume 2. Appendixes A, B, C, D

International Nuclear Information System (INIS)

1995-09-01

This document contains appendices A (water characterization), B (sediment characterization), C (biota Characterization), D (applicable or relevant and appropriate requirements) from the combined Remedial Investigation/Feasibility Study Report for the Clinch River/Poplar Crack (CR/PC) Operable Unit (OU). The CR/PC OU is located in Anderson and Roane Counties, Tennessee and consists of the Clinch River and several of its embayments in Melton Hill and Watts Bar Reservoirs. These waters have received hazardous substances released over a period of 50 years from the US Department of Energy's Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act. A remedial investigation has been conducted to determine the current nature and extent of any contamination and to assess the resulting risk to human health and the environment. The feasibility study evaluates remedial action alternatives to identify any that are feasible for implementation and that would effectively reduce risk. Historical studies had indicated that current problems would likely include 137 Cs in sediment of the Clinch River, mercury in sediment and fish of Poplar Creek and PCBs and pesticides in fish from throughout the OU. Peak releases of mercury and 137 Cs occurred over 35 years ago, and current releases are low. Past releases of PCBs from the ORR are poorly quantified, and current releases are difficult to quantify because levels are so low. The site characterization focused on contaminants in surface water, sediment, and biota. Contaminants in surface water were all found to be below Ambient Water Quality Criteria. Other findings included the following: elevated metals including cesium 137 and mercury in McCoy Branch sediments; PCBs and chlordane elevated in several fish species, presenting the only major human health risk, significant ecological risks in Poplar Creek but not in the Clinch River

Remedial investigation/feasibility study for the Clinch River/Poplar Creek operable unit. Volume 2. Appendixes A, B, C, D

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-09-01

This document contains appendices A (water characterization), B (sediment characterization), C (biota Characterization), D (applicable or relevant and appropriate requirements) from the combined Remedial Investigation/Feasibility Study Report for the Clinch River/Poplar Crack (CR/PC) Operable Unit (OU). The CR/PC OU is located in Anderson and Roane Counties, Tennessee and consists of the Clinch River and several of its embayments in Melton Hill and Watts Bar Reservoirs. These waters have received hazardous substances released over a period of 50 years from the US Department of Energy`s Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act. A remedial investigation has been conducted to determine the current nature and extent of any contamination and to assess the resulting risk to human health and the environment. The feasibility study evaluates remedial action alternatives to identify any that are feasible for implementation and that would effectively reduce risk. Historical studies had indicated that current problems would likely include {sup 137}Cs in sediment of the Clinch River, mercury in sediment and fish of Poplar Creek and PCBs and pesticides in fish from throughout the OU. Peak releases of mercury and {sup 137}Cs occurred over 35 years ago, and current releases are low. Past releases of PCBs from the ORR are poorly quantified, and current releases are difficult to quantify because levels are so low. The site characterization focused on contaminants in surface water, sediment, and biota. Contaminants in surface water were all found to be below Ambient Water Quality Criteria. Other findings included the following: elevated metals including cesium 137 and mercury in McCoy Branch sediments; PCBs and chlordane elevated in several fish species, presenting the only major human health risk, significant ecological risks in Poplar Creek but not in the Clinch River.

The Acute Toxicity of Major Ion Salts to Ceriodaphnia dubia. III. Mathematical models for mixture toxicity

Data.gov (United States)

U.S. Environmental Protection Agency — This dataset concerns the development of models for describing the acute toxicity of major ions to Ceriodaphnia dubia using data from single salt tests and binary...

Biology and taxonomy of Tuthillia cognata (Homoptera : Psylloidea), a pest on Myrciaria dubia (Myrtaceae)

OpenAIRE

Burckhardt, D.; Couturier, Guy

1988-01-01

#Tuthillia cognata$ est découvert comme ravageur de #Myrciaria dubia$ (#Myrtaceae$) au Pérou et Brésil. Tenant compte de la morphologie de la larve, le genre est transféré des #Ciriacreminae$ dans les #Anomoneurinae$. (Résumé d'auteur)

Update of preconstruction radioactivity levels in the vicinity of the proposed Clinch River breeder reactor project

International Nuclear Information System (INIS)

1981-08-01

Routine samples of ground water, river water, and bottom sediment were collected from the Clinch River in 1977 in the preconstruction-construction phase of the CRBRP environmental radiological monitoring program. The results obtained from the analysis of these samples are similar to those reported earlier. The only significant radioisotopes identified in sediment samples were 137 Cs, 60 Co, and the naturally occurring 40 K. Other than for samples collected in November 1976, the results vary from 0.1 to 13.0 pCi/g (dry weight), with concentration generally increasing with distance downstream from CRM 24.0 to CRM 14.4. The extent to which this relationship may or may not hold below CRM 14.4 is beyond the intended scope of this program. No explanation can be given at this time for the elevated levels of 137 Cs detected in November 1976. These values ranged from 28.0 to 82.6 pCi/g (dry weight), with similar values found at CRM 24.0 above Melton Hill Dam. With the exception of tritium, no significant radioactivity was detected in ground or surface water at the CRBRP site. Tritium concentrations ranging from 368 to 5882 pCi/l were found in samples of surface water taken from the Clinch River below Melton Hill Dam while samples taken above the dam exhibited tritium levels from 56 to 368 pCi/l. These elevated tritium levels in the Clinch River below Melton Hill Dam are attributable to DOE operations at Oak Ridge

Indicators of Ceriodaphnia dubia chronic toxicity test performance and sensitivity

Energy Technology Data Exchange (ETDEWEB)

Rosebrock, M.M.; Bedwell, N.J.; Ausley, L.W. [North Carolina Division of Environmental Management, Raleigh, NC (United States)

1994-12-31

The North Carolina Division of Environmental Management has begun evaluation of the sensitivity of test results used for measuring chronic whole effluent toxicity in North Carolina wastewater discharges. Approximately 67% of 565 facilities required to monitor toxicity by an NPDES permit perform a Ceriodaphnia dubia chronic, single effluent concentration (pass/fail) analysis. Data from valid Ceriodaphnia dubia chronic pass/fail tests performed by approximately 20 certified biological laboratories and submitted by North Carolina NPDES permittees were recorded beginning January 1992. Control and treatment reproduction data from over 2,500 tests submitted since 1992 were analyzed to determine the minimum significant difference (MSD) at a 99% confidence level for each test and the percent reduction from the control mean that the MSD represents (%MSD) for each certified laboratory. Initial results for the 20 laboratories indicate that the average intralaboratory percent MSD ranges 12.72% (n = 367) to 34.91% (n = 7) with an average of 23.08%. Additionally, over 3,800 tests were analyzed to determine the coefficient of variation (CV) for control reproduction for each test and the average for each certified biological laboratory. Preliminary review indicates that average interlaboratory control reproduction CV values range from 10.59% (n = 367) to 31.08% (n = 572) with a mean of 20.35%. The statistics investigated are indicators of intra/interlaboratory performance and sensitivity of Ceriodaphnia chronic toxicity analyses.

Reliability modeling of Clinch River breeder reactor electrical shutdown systems

International Nuclear Information System (INIS)

Schatz, R.A.; Duetsch, K.L.

1974-01-01

The initial simulation of the probabilistic properties of the Clinch River Breeder Reactor Plant (CRBRP) electrical shutdown systems is described. A model of the reliability (and availability) of the systems is presented utilizing Success State and continuous-time, discrete state Markov modeling techniques as significant elements of an overall reliability assessment process capable of demonstrating the achievement of program goals. This model is examined for its sensitivity to safe/unsafe failure rates, sybsystem redundant configurations, test and repair intervals, monitoring by reactor operators; and the control exercised over system reliability by design modifications and the selection of system operating characteristics. (U.S.)

Third report on the Oak Ridge National Laboratory Biological Monitoring and Abatement Program for White Oak Creek Watershed and the Clinch River

Energy Technology Data Exchange (ETDEWEB)

Loar, J.M. [ed.; Adams, S.M.; Bailey, R.D. [and others

1994-03-01

As a condition of the National Pollutant Discharge Elimination System (NPDES) permit issued to Oak Ridge National Laboratory (ORNL) on April 1, 1985, a Biological Monitoring and Abatement Program (BMAP) was developed for White Oak Creek (WOC); selected tributaries of WOC, including Fifth Creek, First Creek, Melton Branch, and Northwest Tributary; and the Clinch River. The BMAP currently consists of six major tasks that address both radiological and nonradiological contaminants in the aquatic and terrestrial environs at ORNL. These are (1) toxicity monitoring, (2) bioaccumulation monitoring of nonradiological contaminants in aquatic biota, (3) biological indicator studies, (4) instream ecological monitoring, (5) assessment of contaminants in the terrestrial environment, and (6) radioecology of WOC and White Oak Lake (WOL). The investigation of contaminant transport, distribution, and fate in the WOC embayment-Clinch River-Watts Bar Reservoir system was originally a task of the BMAP but, in 1988, was incorporated into the Resource Conservation and Recovery Act Facility Investigation for the Clinch River, a separate study to assess offsite contamination from all three Department of Energy facilities in Oak Ridge.

Third report on the Oak Ridge National Laboratory Biological Monitoring and Abatement Program for White Oak Creek Watershed and the Clinch River

International Nuclear Information System (INIS)

Loar, J.M.; Adams, S.M.; Bailey, R.D.

1994-03-01

As a condition of the National Pollutant Discharge Elimination System (NPDES) permit issued to Oak Ridge National Laboratory (ORNL) on April 1, 1985, a Biological Monitoring and Abatement Program (BMAP) was developed for White Oak Creek (WOC); selected tributaries of WOC, including Fifth Creek, First Creek, Melton Branch, and Northwest Tributary; and the Clinch River. The BMAP currently consists of six major tasks that address both radiological and nonradiological contaminants in the aquatic and terrestrial environs at ORNL. These are (1) toxicity monitoring, (2) bioaccumulation monitoring of nonradiological contaminants in aquatic biota, (3) biological indicator studies, (4) instream ecological monitoring, (5) assessment of contaminants in the terrestrial environment, and (6) radioecology of WOC and White Oak Lake (WOL). The investigation of contaminant transport, distribution, and fate in the WOC embayment-Clinch River-Watts Bar Reservoir system was originally a task of the BMAP but, in 1988, was incorporated into the Resource Conservation and Recovery Act Facility Investigation for the Clinch River, a separate study to assess offsite contamination from all three Department of Energy facilities in Oak Ridge

EFFECTIVE CONCENTRATIONS OF 6 CONTAMINANTS TO LEMMA MINOR, PIMEPHALES PROMELA, DAPHNIA MAGNA, AND CERIODAPHNIA DUBIA

Science.gov (United States)

The research presented here resulted in EC50 and LOEC values for the contaminants copper, cadmium, diazinon, atrazine, and cyanide to the species Lemna Minor, Pimephales promelas, Daphnia magna, and Ceriodaphnia dubia. Observed values were used as benchmarks for assessing the se...

Numerical Analysis Of The Resistance To Pullout Test Of Clinched Assemblies Of Thin Metal Sheets

International Nuclear Information System (INIS)

Jomaa, Moez; Billardon, Rene

2007-01-01

This paper presents the finite element analysis of the resistance of a clinch point to pullout test -that follows the numerical analysis of the forming process of the point-. The simulations have been validated by comparison with experimental evidences. The influence on the numerical predictions of various computation and process parameters have been evaluated

Present day design challenges exemplified by the Clinch River Breeder Reactor Plant

International Nuclear Information System (INIS)

Dickson, P.W. Jr.; Anderson, C.A. Jr.

1976-01-01

The present day design challenges faced by the Clinch River Breeder Reactor Plant engineer result from two causes. The first cause is aspiration to achieve a design that will operate at conditions which are desirable for future LMFBRs in order for them to achieve low power costs and good breeding. The second cause is the licensing impact. Although licensing the CRBRP won't eliminate future licensing effort, many licensing questions will have been resolved and precedents set for the future LMFBR industry

Conceptual design of the Clinch River Breeder Reactor spent-fuel shipping cask

International Nuclear Information System (INIS)

Pope, R.B.; Diggs, J.M.

1982-04-01

Details of a baseline conceptual design of a spent fuel shipping cask for the Clinch River Breeder Reactor (CRBR) are presented including an assessment of shielding, structural, thermal, fabrication and cask/plant interfacing problems. A basis for continued cask development and for new technological development is established. Alternates to the baseline design are briefly presented. Estimates of development schedules, cask utilization and cost schedules, and of personnel dose commitments during CRBR in-plant handling of the cask are also presented

Remedial investigation/feasibility study of the Clinch River/Poplar Creek operable unit. Volume 1: Main text

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-06-01

This report presents the findings of an investigation into contamination of the Clinch River and Poplar Creek near the US Department of Energy`s (DOE`s) Oak Ridge Reservation (ORR) in eastern Tennessee. For more than 50 years, various hazardous and radioactive substances have been released to the environment as a result of operations and waste management activities at the ORR. In 1989, the ORR was placed on the National Priorities List (NPL), established and maintained under the federal Comprehensive environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Under CERCLA, NPL sites must be investigated to determine the nature and extent of contamination at the site, assess the risk to human health and the environment posed by the site, and, if necessary, identify feasible remedial alternatives that could be used to clean the site and reduce risk. To facilitate the overall environmental restoration effort at the ORR, CERCLA activities are being implemented individually as distinct operable units (OUs). This document is the combined Remedial Investigation and Feasibility Study Report for the Clinch River/Poplar Creek OU.

Morphological and genetic diversity of camu-camu [Myrciaria dubia (Kunth) McVaugh] in the Peruvian Amazon

Czech Academy of Sciences Publication Activity Database

Šmíd, J.; Kalousková, M.; Mandák, Bohumil; Houška, Jakub; Chládková, A.; Pinedo, M.; Lojka, B.

2017-01-01

Roč. 12, č. 6 (2017), s. 1-15, č. článku e0179886. E-ISSN 1932-6203 Institutional support: RVO:67985939 Keywords : Myricaria dubia * genetic diversity * Amazonia Subject RIV: EF - Botanics OBOR OECD: Plant sciences, botany Impact factor: 2.806, year: 2016

Camu-camu propagation (Myrciaria dubia (H.B.K. Mcvaugh = Propagação do camu-camu (Myrciaria dubia (H.B.K. Mcvaugh

Directory of Open Access Journals (Sweden)

Edvan Alves Chagas

2012-04-01

Full Text Available Camu-camu is an indigenous species of the Brazilian Amazon region, and is found in the states of Para, Amazonas, Roraima, Rondônia and Tocantins. Domestication of Myrciaria dubia has been performed by several Brazilian institutions for decades and is desirable to develop a propagation method to obtain good quality seedlings and the multiplication of genotypes from plants selection with desirable characteristics. Thus, this review contains the main research results of the vegetative and seed propagation of camu-camu, having important information to be applied on seedlings and cuttings management in a nursery. = O camu-camu é uma espécie nativa da região Amazônica, podendo ser encontrada em diversos estados da Região Norte do Brasil, como nos estados do Pará, Amazonas, Roraima, Rondônia e Tocantins. Os trabalhos de domesticação e melhoramento genético de Myrciaria dubia têm sido realizados por diversas instituições brasileiras há várias décadas e sabese que para isso são necessários inicialmente estudos relativos à propagação da espécie para obtenção de mudas de qualidade e multiplicação de genótipos superiores provenientes de plantas selecionadas e com características agronômicas, como alta produção de frutos e elevado teor de vitamina C. Sendo assim, esta revisão reúne resultados das principais pesquisas sobre a propagação vegetativa e seminífera do camu-camu, trazendo informações importantes de manejo que podem ser aplicadas à produção de mudas.

Individual- and population-level toxicity of the insecticide, spirotetramat and the agricultural adjuvant, Destiny to the Cladoceran, Ceriodaphnia dubia.

Science.gov (United States)

Chen, Xue Dong; Stark, John D

2010-08-01

The effects of the tetramic acid insecticide, spirotetramat and the agricultural adjuvant, Destiny, were evaluated on the Cladoceran, Ceriodaphnia dubia. These compounds were evaluated separately and as a mixture because they can be applied together for control of certain crop pests and therefore have the potential to enter surface water as a binary mixture. Acute mortality estimates (48 h) were developed followed by chronic exposure (8 days) studies where several population parameters were recorded. Acute LC50 and 95% CL for spirotetramat and Destiny were estimated to be 23.8 (14.5-35.4) and 26.71 (20.8-34.0) mg/l, respectively. Thus, spirotetramat and Destiny were equitoxic to C. dubia at LC50. For the chronic population study, C. dubia populations were exposed to a range of concentrations for spirotetramat and Destiny singly and as a mixture. Each chemical alone reduced the number of founding individuals, offspring/female, final population size, and population growth rate in a concentration-dependent manner. However, exposure to the mixture caused significantly greater reductions in these parameters than either compound alone. These results indicate that agricultural adjuvants and pesticides may cause more damage to aquatic organisms as a mixture than either product alone. Therefore, future evaluations of pesticide effects should consider the effects of adjuvants as a mixture with pesticides when these products are recommended to be applied together for pest control.

AISLAMIENTO DE ADN GENÓMICO DE Myrciaria dubia (HBK “CAMU CAMU” APROPIADO PARA ANÁLISIS MOLECULARES

Directory of Open Access Journals (Sweden)

Juan Castro Gómez

2012-06-01

Full Text Available Myrciaria dubia “camu-camu”, una especie nativa de la Amazonía que produce frutos con alto contenido de vitamina C y otras sustancias importantes. Sin embargo, los estudios moleculares de esta planta son escasos, por falta de un protocolo reproducible para purificar sus ácidos nucléicos. El objetivo de este trabajo fue establecer un protocolo para aislar el ADN genómico a partir de hojas de M. dubia, apropiado para análisis moleculares. El ADN se purificó con un protocolo modificado, la calidad y cantidad se estimó por espectrofotometría y electroforesis en gel de agarosa. Adicionalmente, la calidad se evaluó mediante RAPD. El ratio de calidad (A260/A280 promedio del ADN fue 1.9±0.1 y el espectro de absorción UV/Vis presentó un único pico de máxima absorbancia a 260nm. Mediante electroforesis el ADN fue íntegro y sin ARN. También, la síntesis de amplicones RAPD nos sugiere ausencia de inhibidores para polimerasas. La concentración promedio del ADN fue 99±33 ng/ml y el rendimiento promedio fue 237±80 mg ADN/g hoja. En conclusión, se ha establecido un protocolo de aislamiento de ADN genómico a partir de hojas de Myrciaria dubia “camu camu”, caracterizado por permitirnos obtener ADN de alta calidad y cantidad suficiente para análisis moleculares como el RAPD.

Dietary camu camu, Myrciaria dubia, enhances immunological response in Nile tilapia.

Science.gov (United States)

Yunis-Aguinaga, Jefferson; Fernandes, Dayanne C; Eto, Silas F; Claudiano, Gustavo S; Marcusso, Paulo F; Marinho-Neto, Fausto A; Fernandes, João B K; de Moraes, Flávio R; de Moraes, Julieta R Engrácia

2016-11-01

Camu camu, Myrciaria dubia, is an Amazon plant that presents high levels of vitamin C in its composition. Several studies in animals and humans have demonstrated their efficiency in the prevention and treatment of various diseases. However, there are no reports of its properties in fish. The aim of this study was to evaluate the effect of the oral administration of the extract of this plant in the immune parameters in Nile tilapia, Oreochromis niloticus. 400 Nile tilapia (80 ± 5 g) were randomly distributed into 20 tanks with 1500 L capacity each (20 fish/tank). After a week of adaptation to environmental conditions, it was provided a diet for 5 weeks, using different levels of inclusion of camu camu extract: 0, 50, 100, 250, and 500 mg/kg of feed. Each treatment consisted of four replicates. It was obtained 40.5 mg of vitamin C/g of camu camu pulp powder by high-performance liquid chromatography. At the end of the trial period, fish were inoculated with Aeromonas hydrophila in the swim bladder. Samples were taken after 6; 24 and 48 h of the challenge. Results revealed that fish supplemented with this herb showed significant increase (P  0.05). No histopathological lesions were observed in intestine, kidney, spleen, and gills. It can be concluded that the addition of Myrciaria dubia in tilapia feed improves the immune response and the growth after 5 weeks, especially, at a dose of 500 mg/kg. Copyright © 2016. Published by Elsevier Ltd.

Assessment of potential impact of the Clinch River Breeder Reactor Plant thermal effluent on the Watts Bar Reservoir striped bass population

International Nuclear Information System (INIS)

Heuer, J.H.; McIntosh, D.; Ostrowski, P.; Tomljanovich, D.A.

1983-11-01

This report is an assessment of potential adverse impact to striped bass (Morone saxatilis) in Watts Bar Reservoir caused by thermal effluent from operation of the Clinch River Breeder Reactor Plant (CRBRP). The Clinch River arm of Watts Bar Reservoir is occupied by adult striped bass during the warmest months of the year. Concern was raised that operation of the CRBRP, specifically thermal discharges, could conflict with management of striped bass. In all cases examined the thermal plume becomes nearly imperceptible within a short distance from the discharge pipe (about 30 ft [10 m]) compared to river width (about 630 ft [190 m]). Under worst case conditions any presence of the plume in the main channel (opposite side of the river from the discharge) will be confined to the surface layer of the water. An ample portion of river cross sections containing ambient temperature water for passage or residence of adult striped bass will always be available in the vicinity of this thermal effluent. Although a small portion of river cross section would exceed the thermal tolerance of striped bass, the fish would naturally avoid this area and seek out adjacent cooler water. Therefore, it is concluded the CRBRP thermal effluent will not significantly affect the integrity of the striped bass thermal refuge in the Clinch River arm of Watts Bar Reservoir. At this time there is no need to consider alternative diffuser designs and thermal modeling. 8 references, 3 figures, 2 tables

Quality assurance/quality control summary report for Phase 1 of the Clinch River remedial investigation. Environmental Restoration Program

International Nuclear Information System (INIS)

Holladay, S.K.; Bevelhimer, M.S.; Brandt, C.C.

1994-07-01

The Clinch River Remedial Investigation (CRRI) is designed to address the transport, fate, and distribution of waterborne contaminants released from the US Department of Energy Oak Ridge Reservation and to assess potential risks to human health and the environment associated with these contaminants. Primary areas of investigation are Melton Hill Reservoir, the Clinch River from Melton Hill Dam to its confluence with the Tennessee River, Poplar Creek, and Watts Bar Reservoir. Phase 1 of the CRRI was a preliminary study in selected areas of the Clinch River/Watts Bar Reservoir. Fish, sediment, and water samples were collected and analyzed for inorganic, organic, and radiological parameters. Phase 1 was designed to (1) obtain high-quality data to confirm existing historical data for contaminant levels; (2) determine the range of contaminant concentrations present in the river-reservoir system; (3) identify specific contaminants of concern; and (4) establish the reference (background) concentrations for those contaminants. Quality assurance (QA) objectives for Phase I were that (1) scientific data generated would withstand scientific scrutiny; (2) data would be gathered using appropriate procedures for field sampling, chain-of-custody, laboratory analyses, and data reporting; and (3) data would be of known precision and accuracy. These objectives were met through the development and implementation of (1) a QA oversight program of audits and surveillances; (2) standard operating procedures accompanied by a training program; (3) field sampling and analytical laboratory quality control requirements; (4) data and records management systems; and (5) validation of the data by an independent reviewer. Approximately 1700 inorganic samples, 1500 organic samples, and 2200 radiological samples were analyzed and validated. The QA completeness objective for the project was to obtain valid analytical results for at least 95% of the samples collected

The Effects of Elevated Specific Conductivity on the Chronic Toxicity of Mining Influenced Streams Using Ceriodaphnia dubia.

Science.gov (United States)

Armstead, Mindy Yeager; Bitzer-Creathers, Leah; Wilson, Mandee

2016-01-01

Salinization of freshwater ecosystems as a result of human activities has markedly increased in recent years. Much attention is currently directed at evaluating the effects of increased salinity on freshwater biota. In the Central Appalachian region of the eastern United States, specific conductance from alkaline discharges associated with mountain top mining practices has been implicated in macroinvertebrate community declines in streams receiving coal mining discharges. Whole effluent toxicity testing of receiving stream water was used to test the hypothesis that mine discharges are toxic to laboratory test organisms and further, that toxicity is related to ionic concentrations as indicated by conductivity. Chronic toxicity testing using Ceriodaphnia dubia was conducted by contract laboratories at 72 sites with a total of 129 tests over a 3.5 year period. The database was evaluated to determine the ionic composition of mine effluent dominated streams and whether discharge constituents were related to toxicity in C. dubia. As expected, sulfate was found to be the dominant anion in streams receiving mining discharges with bicarbonate variable and sometimes a substantial component of the dissolved solids. Overall, the temporal variability in conductance was low at each site which would indicate fairly stable water quality conditions. Results of the toxicity tests show no relationship between conductance and survival of C. dubia in the mining influenced streams with the traditional toxicity test endpoints. However, consideration of the entire dataset revealed a significant inverse relationship between conductivity and neonate production. While conductivity explained very little of the high variability in the offspring production (r2 = 0.1304), the average numbers of offspring were consistently less than 20 neonates at the highest conductivities.

The Effects of Elevated Specific Conductivity on the Chronic Toxicity of Mining Influenced Streams Using Ceriodaphnia dubia.

Directory of Open Access Journals (Sweden)

Mindy Yeager Armstead

Full Text Available Salinization of freshwater ecosystems as a result of human activities has markedly increased in recent years. Much attention is currently directed at evaluating the effects of increased salinity on freshwater biota. In the Central Appalachian region of the eastern United States, specific conductance from alkaline discharges associated with mountain top mining practices has been implicated in macroinvertebrate community declines in streams receiving coal mining discharges. Whole effluent toxicity testing of receiving stream water was used to test the hypothesis that mine discharges are toxic to laboratory test organisms and further, that toxicity is related to ionic concentrations as indicated by conductivity. Chronic toxicity testing using Ceriodaphnia dubia was conducted by contract laboratories at 72 sites with a total of 129 tests over a 3.5 year period. The database was evaluated to determine the ionic composition of mine effluent dominated streams and whether discharge constituents were related to toxicity in C. dubia. As expected, sulfate was found to be the dominant anion in streams receiving mining discharges with bicarbonate variable and sometimes a substantial component of the dissolved solids. Overall, the temporal variability in conductance was low at each site which would indicate fairly stable water quality conditions. Results of the toxicity tests show no relationship between conductance and survival of C. dubia in the mining influenced streams with the traditional toxicity test endpoints. However, consideration of the entire dataset revealed a significant inverse relationship between conductivity and neonate production. While conductivity explained very little of the high variability in the offspring production (r2 = 0.1304, the average numbers of offspring were consistently less than 20 neonates at the highest conductivities.

Ciclo biológico, comportamiento y censo del picudo del camu camu, Conotrachelus dubiae O'Brien 1995 (Coleoptera: Curculionidae en Pucallpa, Perú Biological cycle, behavior and census of camu camu weevil, Conotrachelus dubiae O'Brien 1995 (Coleoptera: Curculionidae, in Pucallpa, Peru

Directory of Open Access Journals (Sweden)

Diana Perez

2008-01-01

Full Text Available El picudo, Conotrachelus dubiae O'Brien 1995, es una de las plagas mas importantes del camu camu Myrciaria dubia H.B.K. Mc Vaugh en la Amazonía Peruana. El objetivo del presente estudio fue determinar el ciclo biológico de este insecto bajo condiciones de laboratorio y describir su comportamiento y fluctuación en condiciones de campo en Pucallpa, Ucayali, Perú. El porcentaje de eclosión de larvas fue de 87%, la duración del periodo de incubación de los huevos fue de 5,5±0,9 (4 a 7 días, del estado larval en el fruto 22,2±1,9 (20 a 25 días y en el suelo (fase pre-pupa, 54,4±5,5 (46 a 67 días, del periodo pupal 11,8±0,9 (9 a 13 días y la longevidad del adulto fue de 51,8±18,9 (9 a 75 días. Los adultos se alimentaron de frutos de diferentes diámetros y estados de maduración y de botones florales, ramas tiernas y flores. No se registró la presencia de adultos de C. dubiae en frutos secos, ni en la base del tallo, sino en ritidomas. La mayor actividad de alimentación y de reproducción de los adultos fue entre 18:30 a 22:00 h. Los adultos fueron observados en el cultivo durante todo el año, encontrándose con mayor frecuencia en los meses de enero a marzo en pisos bajos inundables y entre octubre a diciembre en tierra firme no inundable, coincidiendo con la fase de floración y fructificación de la planta.Camu camu weevil Conotrachelus dubiae O'Brien, 1995 is a one of the main pests of camu camu (Myrciaria dubia H.B.K. Mc Vaugh in Peruvian Amazonia. The aim of this study was to determine the biological cycle of this insect under laboratory conditions, to describe its behavior and population numbers under field conditions in Pucallpa, Ucayali, Peru. The percentage of hatching was 87%; the egg incubation period was 5.5±0.9 (4 to 7 days; the length of the larval stage inside the fruit was 22.2±1.9 (20 to 25 days, and the length larval stage (pre-pupa underground was 54.4±5.5 (46 to 67 days. The length of pupal period was 11.8�

Seismic design criteria for the Clinch River Breeder Reactor Plant

International Nuclear Information System (INIS)

Morrone, A.; Bitner, J.L.; Sigal, G.B.

1975-01-01

The general criteria for seismic resistant design for structures, systems and components of the Clinch River Breeder Reactor Plant (CRBRP) are presented and discussed. Site dependency of the maximum ground accelerations for the Operating Basis Earthquake and the Safe Shutdown Earthquake is described from the viewpoint of historical records and geological and seismological studies for the CRBRP site. The respective ground response spectra are derived by normalization of the latest AEC Regulatory standard shapes to these maximum ground accelerations. Modeling and analytical techniques and requirements are given. In addition, loading conditions and categories, loading combinations, earthquake direction effects and allowable damping values are defined. A discussion of the testing criteria which considers both single and multiple frequency test motions, and basic test procedures for single frequency sine beat testing is presented. (U.S.)

Thermal insulation system design and fabrication specification (nuclear) for the Clinch River Breeder Reactor plant

International Nuclear Information System (INIS)

1978-01-01

This specification defines the design, analysis, fabrication, testing, shipping, and quality requirements of the Insulation System for the Clinch River Breeder Reactor Plant (CRBRP), near Oak Ridge, Tennessee. The Insulation System includes all supports, convection barriers, jacketing, insulation, penetrations, fasteners, or other insulation support material or devices required to insulate the piping and equipment cryogenic and other special applications excluded. Site storage, handling and installation of the Insulation System are under the cognizance of the Purchaser

Quality assurance in technology development for The Clinch River Breeder Reactor Plant Project

International Nuclear Information System (INIS)

Anderson, J.W.

1980-01-01

The Clinch River Breeder Reactor Plant Project is the nation's first large-scale demonstration of the Liquid Metal Fast Breeder Reactor (LMFBR) concept. The Project has established an overall program of plans and actions to assure that the plant will perform as required. The program has been established and is being implemented in accordance with Department of Energy Standard RDT F 2-2. It is being applied to all parts of the plant, including the development of technology supporting its design and licensing activity. A discussion of the program as it is applied to development is presented

Remedial investigation/feasibility study of the Clinch River/Poplar Creek operable unit. Volume 5

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-03-01

This volume is in support of the findings of an investigation into contamination of the Clinch River and Poplar Creek near the Oak Ridge Reservation (for more than 50 years, various hazardous and radioactive substances have been released to the environment as a result of operations and waste management activities there). It addresses the quality assurance objectives for measuring the data, presents selected historical data, contains data from several discrete water characterization studies, provides data supporting the sediment characterization, and contains data related to several biota characterization studies.

Structural analysis of the Upper Internals Structure for the Clinch River Breeder Reactor Plant

International Nuclear Information System (INIS)

Houtman, J.L.

1979-01-01

The Upper Internals Structure (UIS) of the Clinch River Breeder Reactor Plant (CRBRP) provides control of core outlet flow to prevent severe thermal transients from occuring at the reactor vessel and primary heat transport outlet piping, provides instrumentation to monitor core performance, provides support for the control rod drivelines, and provides secondary holddown of the core. All of the structural analysis aspects of assuring the UIS is structurally adequate are presented including simplified and rigorous inelastic analysis methods, elevated temperature criteria, environmental effects on material properties, design techniques, and manufacturing constraints

Clinch river breeder reactor plant steam generator water quality

Energy Technology Data Exchange (ETDEWEB)

Van Hoesen, D; Lowe, P A

1975-07-01

The recent problems experienced by some LWR Steam Generators have drawn attention to the importance of system water quality and water/ steam side corrosion. Several of these reactor plants have encountered steam generator failures due to accelerated tube corrosion caused, in part, by poor water quality and corrosion control. The CRBRP management is aware of these problems, and the implications that they have for the Clinch River Breeder Reactor Plant (CPBRP) Steam Generator System (SGS). Consequently, programs are being implemented which will: (1) investigate the corrosion mechanisms which may be present in the CRBRP SGS; (2) assure steam generator integrity under design and anticipated off-normal water quality conditions; and (3) assure that the design water quality levels are maintained at all times. However, in order to understand the approach being used to examine this potential problem, it is first necessary to look at the CRBRP SGS and the corrosion mechanisms which may be present.

Clinch river breeder reactor plant steam generator water quality

International Nuclear Information System (INIS)

Van Hoesen, D.; Lowe, P.A.

1975-01-01

The recent problems experienced by some LWR Steam Generators have drawn attention to the importance of system water quality and water/ steam side corrosion. Several of these reactor plants have encountered steam generator failures due to accelerated tube corrosion caused, in part, by poor water quality and corrosion control. The CRBRP management is aware of these problems, and the implications that they have for the Clinch River Breeder Reactor Plant (CPBRP) Steam Generator System (SGS). Consequently, programs are being implemented which will: 1) investigate the corrosion mechanisms which may be present in the CRBRP SGS; 2) assure steam generator integrity under design and anticipated off-normal water quality conditions; and 3) assure that the design water quality levels are maintained at all times. However, in order to understand the approach being used to examine this potential problem, it is first necessary to look at the CRBRP SGS and the corrosion mechanisms which may be present

Development and validation of a chronic copper biotic ligand model for Ceriodaphnia dubia

International Nuclear Information System (INIS)

Schwartz, Melissa L.; Vigneault, Bernard

2007-01-01

A biotic ligand model (BLM) to predict chronic Cu toxicity to Ceriodaphnia dubia was developed and tested. The effect of cationic competition, pH and natural organic matter complexation of Cu was examined to develop the model. There was no effect of cationic competition using increasing Ca and Na concentrations in our exposures. However, we did see a significant regression of decreasing toxicity (measured as the IC25; concentration at which there was a 25% inhibition of reproduction) as Mg concentration increased. However, taking into account the actual variability of the IC25 and since the relative increase in IC25 due to additional Mg was small (1.5-fold) Mg competition was not included in the model. Changes in pH had a significant effect on Cu IC25, which is consistent with proton competition as often suggested for acute BLMs. Finally, natural organic matter (NOM) was added to exposures resulting in significant decreases in toxicity. Therefore, our predictive model for chronic Cu toxicity to C. dubia includes the effect of pH and NOM complexation. The model was validated with Cu IC25 data generated in six natural surface waters collected from across Canada. Using WHAM VI, we calculated Cu speciation in each natural water and using our model, we generated 'predicted' IC25 data. We successfully predicted all Cu IC25 within a factor of 3 for the six waters used for validation

Production of artemisinin and its derivatives in hairy roots of Artemisia dubia induced by rolA gene transformation

International Nuclear Information System (INIS)

Amanullah, M.; Mirza, B.; Zia, M.

2016-01-01

Artemisinin and its derivatives are phytochemical constituents of genus Artemisia. Demand of these plant secondary metabolitesis increasing due to their immense therapeutic significance. Besides their established antimalarial role, recent studies have also disclosed their anticancer potentials. It has made imperative to develop new and efficient sources of these compounds. Inherent synthetic challenges give biological sources preference over chemical synthesis of artemisinin and its derivatives. Therefore, genetic improvement of plants and, rather less preferentially, microbes is focus of current research to gain increase productivity of these valuable drugs. This study has analyzed A. dubiaas potential source of artemisinin and its derivatives. Transformation of Artemisia dubia was carried out using A. tumefaciens strain LBA 4404 containing rolA gene constructed on pRB 29. Healthy and acclimatizable transgenic plants were produced using optimized concentrations of BAP and NAA. Previously acclimatized rol ABC transgenic plants were also In vitro regenerated for comparative analysis of artemisinin and its derivatives. PCR amplification of rolA gene was done to confirm the integration of T-DNA in transgenic plants.TLC analysis was performed to evaluate comparative production of artemisinin and derivatives in rolA and rol ABC transgenic A. dubia. It revealed that rolA transgenic plants contain comparable amounts of these metabolites. Both type of transgenic plants manifested the enhancement of other uncharacterized compounds as well. Besides systematic optimization of In vitro regenerative protocol for Artemisia dubia, relative regeneration ability of rol transgenic and controlplants was also assessed at four regenerative stages. It was observed that unlike control, rol transgenic plants showed best root induction only on combination of auxins and cytokines. It was concluded that rol genes transformation of plants is an efficient tool to enhance their secondary

Technical management plan for sample generation, analysis, and data review for Phase 2 of the Clinch River Environmental Restoration Program

International Nuclear Information System (INIS)

Brandt, C.C.; Benson, S.B.; Beeler, D.A.

1994-03-01

The Clinch River Remedial Investigation (CRRI) is designed to address the transport, fate, and distribution of waterborne contaminants (radionuclides, metals, and organic compounds) released from the US Department of Energy's (DOE's) Oak Ridge Reservation (ORR) and to assess potential risks to human health and the environment associated with these contaminants. The remedial investigation is entering Phase 2, which has the following items as its objectives: define the nature and extent of the contamination in areas downstream from the DOE ORR, evaluate the human health and ecological risks posed by these contaminants, and perform preliminary identification and evaluation of potential remediation alternatives. This plan describes the requirements, responsibilities, and roles of personnel during sampling, analysis, and data review for the Clinch River Environmental Restoration Program (CR-ERP). The purpose of the plan is to formalize the process for obtaining analytical services, tracking sampling and analysis documentation, and assessing the overall quality of the CR-ERP data collection program to ensure that it will provide the necessary building blocks for the program decision-making process

Remedial investigation/feasibility study of the Clinch River/Poplar Creek Operable Unit. Volume 3. Risk assessment information. Appendixes E, F

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-03-01

This report presents the findings of an investigation into contamination of the Clinch River and Poplar Creek near the U.S. Department of Energy`s (DOE`s) Oak Ridge Reservation (ORR) in eastern Tennessee. For more than 50 years, various hazardous and radioactive substances have been released to the environment as a result of operations and waste management activities at the ORR. In 1989, the ORR was placed on the National Priorities List (NPL), established and maintained under the federal Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Under CERCLA, NPL sites must be investigated to determine the nature and extent of contamination at the site, assess the risk to human health and the environment posed by the site, and, if necessary, identify feasible remedial alternatives that could be used to clean the site and reduce risk. To facilitate the overall environmental restoration effort at the ORR, CERCLA activities are being implemented individually as distinct operable units (OUs). This document is Volume 3 of the combined Remedial Investigation and Feasibility Study Report for the Clinch River/Poplar Creek OU.

Second report on the Oak Ridge National Laboratory Biological Monitoring and Abatement Program for White Oak Creek Watershed and the Clinch River

International Nuclear Information System (INIS)

Loar, J.M.; Appellanis, S.M.; Jimenez, B.D.; Huq, M.V.; Meyers-Schone, L.J.; Mohrbacher, D.A.; Olsen, C.R.

1992-12-01

As a condition of the National Pollutant Discharge Elimination System (NPDES) permit issued to Oak Ridge National Laboratory (ORNL) on April 1, 1986, a Biological Monitoring and Abatement Program (BMAP) was developed for White Oak Creek (WOC); selected tributaries of WOC, including Fifth Creek, First Creek, Melton Branch, and Northwest Tributary; and the Clinch River. BMAP consists of seven major tasks that address both radiological and nonradiological contaminants in the aquatic and terrestrial environs on-site and the aquatic environs off-site. These tasks are (1) toxicity monitoring; (2) bioaccumulation monitoring of nonradiological contaminants in aquatic biota; (3) biological indicator studies; (4) instream ecological monitoring; (5) assessment of contaminants in the terrestrial environment; (6) radioecology of WOC and White Oak Lake (WOL); and (7) contaminant transport, distribution, and fate in the WOC embayment-Clinch River-Watts Bar Reservoir system. This document, the second of a series of annual reports, described the results of BMAP studies conducted in 1987

Second report on the Oak Ridge National Laboratory Biological Monitoring and Abatement Program for White Oak Creek Watershed and the Clinch River

Energy Technology Data Exchange (ETDEWEB)

Loar, J.M. [ed.; Adams, S.M.; Bailey, R.D.; Blaylock, B.G.; Boston, H.L.; Cox, D.K.; Huston, M.A.; Kimmel, B.L.; Loar, J.M.; Olsen, C.R.; Ryon, M.G.; Shugart, L.R.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.; Walton, B.T.; Talmage, S.S.; Murphy, J.B.; Valentine, C.K. [Oak Ridge National Lab., TN (United States); Appellanis, S.M.; Jimenez, B.D. [Puerto Rico Univ., San Juan (Puerto Rico); Huq, M.V. [Connecticut Dept. of Environmental Protection, Hamden, CT (United States); Meyers-Schone, L.J. [Frankfurter, Gross-Gerau (Germany); Mohrbacher, D.A. [Automated Sciences Group, Inc., Oak Ridge, TN (United States); Olsen, C.R. [USDOE Office of Energy Research, Washington, DC (United States). Environmental Sciences Div.; Stout, J.G. [Cincinnati Univ., OH (United States)

1992-12-01

As a condition of the National Pollutant Discharge Elimination System (NPDES) permit issued to Oak Ridge National Laboratory (ORNL) on April 1, 1986, a Biological Monitoring and Abatement Program (BMAP) was developed for White Oak Creek (WOC); selected tributaries of WOC, including Fifth Creek, First Creek, Melton Branch, and Northwest Tributary; and the Clinch River. BMAP consists of seven major tasks that address both radiological and nonradiological contaminants in the aquatic and terrestrial environs on-site and the aquatic environs off-site. These tasks are (1) toxicity monitoring; (2) bioaccumulation monitoring of nonradiological contaminants in aquatic biota; (3) biological indicator studies; (4) instream ecological monitoring; (5) assessment of contaminants in the terrestrial environment; (6) radioecology of WOC and White Oak Lake (WOL); and (7) contaminant transport, distribution, and fate in the WOC embayment-Clinch River-Watts Bar Reservoir system. This document, the second of a series of annual reports, described the results of BMAP studies conducted in 1987.

Conservação de sementes de Myrciaria dubia (H.B.K. McVaugh Myrciaria dubia (H.B.K. McVaugh seed storability as affected by temperature and seed moisture content

Directory of Open Access Journals (Sweden)

Daniel Felipe de Oliveira Gentil

2004-12-01

Full Text Available As sementes de Myrciaria dubia apresentam baixa longevidade e demandam a ampliação do conhecimento sobre fatores interferentes na sua conservação. Assim, o objetivo deste trabalho foi verificar as influências do grau de umidade e da temperatura do ambiente na manutenção da qualidade dessas sementes. Para tanto, sementes com 48%, 43%, 40%, 34%, 30%, 24%, 18% e 14% de água, acondicionadas em sacos de polietileno, foram armazenadas sob temperaturas controladas de 10 ºC, 20 ºC e 30 ºC, durante 280 dias, e submetidas, periodicamente, à avaliação do grau de umidade, da germinação, do vigor e da sanidade. Constatou-se que as sementes têm a conservação favorecida pela associação do grau de umidade de 43% com a temperatura de armazenamento de 10 °C.Myrciaria dubia seeds have a short life span and few informations are available about the factors that determine their storability. The main aim of this research work was to check the effects of three temperatures (10 ºC, 20 ºC and 30 ºC and eight seed moisture contents (48%, 43%, 40%, 34%, 30%, 24%, 18% and 14% on seed storability, during a time period of 280 days. Periodic evaluations of seed moisture content, germination, vigor and healthness were carried out throughout the experimental time. The best seed storability conditions were observed at 10 °C and 43% of seed moisture content.

Inducción de la biosíntesis local de antocianinas en frutos de Myrciaria dubia mediante lesiones mecánicas

Directory of Open Access Journals (Sweden)

Daniel Motta Santillán

2014-12-01

Full Text Available En las plantas las antocianinas cumplen roles fundamentales en sus interacciones con el medio ambiente, son una de las líneas de defensa contra los radicales libres, la radiación ultravioleta y el ataque de patógenos. Observaciones realizadas muestran que los frutos de M. dubia acumulan antocianinas alrededor de lesiones mecánicas probablemente causadas por insectos. Por tanto, hemos probado la hipótesis que produciendo lesiones mecánicas en frutos verdes de M. dubia se induce la biosíntesis local de antocianinas. De tres plantas en fructificación de la colección de germoplasma del INIA se seleccionaron aleatoriamente 40 frutos verdes (25 ± 5 mm de Ø y se causaron lesiones mecánicas con estilete o bisturí al 75% de ellos. Posteriormente, se registró el porcentaje de acumulación de antocianinas por área superficial del fruto a las 0, 24, 48 y 72 horas. Se observó acumulación de antocianinas en ~10% (24 horas, ~30% (48 horas y ~70% (72 horas del área superficial de los frutos. En conclusión, las lesiones mecánicas causadas en frutos de M. dubia inducen la biosíntesis local de antocianinas, probablemente como un mecanismo de defensa efectivo desarrollado por esta especie. La comprensión de los mecanismos moleculares implicados en esta respuesta fisiológica nos permitirá establecer las bases para la mejora genética de esta especie.

Inducción de la biosíntesis local de antocianinas en frutos de Myrciaria dubia mediante lesiones mecánicas

Directory of Open Access Journals (Sweden)

Daniel Motta-Santillán

2015-01-01

Full Text Available En las plantas las antocianinas cumplen roles fundamentales en sus interacciones con el medio ambiente, son una de las líneas de defensa contra los radicales libres, la radiación ultravioleta y el ataque de patógenos. Observaciones realizadas muestran que los frutos de M. dubia acumulan antocianinas alrededor de lesiones mecánicas probablemente causadas por insectos. Por tanto, hemos probado la hipótesis que produciendo lesiones mecánicas en frutos verdes de M. dubia se induce la biosíntesis local de antocianinas. De tres plantas en fructificación de la colección de germoplasma del INIA se seleccionaron aleatoriamente 40 frutos verdes (25 ± 5 mm de Ø y se causaron lesiones mecánicas con estilete o bisturí al 75% de ellos. Posteriormente, se registró el porcentaje de acumulación de antocianinas por área superficial del fruto a las 0, 24, 48 y 72 horas. Se observó acumulación de antocianinas en ~10% (24 horas, ~30% (48 horas y ~70% (72 horas del área superficial de los frutos. En conclusión, las lesiones mecánicas causadas en frutos de M. dubia inducen la biosíntesis local de antocianinas, probablemente como un mecanismo de defensa efectivo desarrollado por esta especie. La comprensión de los mecanismos moleculares implicados en esta respuesta fisiológica nos permitirá establecer las bases para la mejora genética de esta especie.

Remedial investigation/feasibility study of the Clinch River/Poplar Creek operable unit. Volume 3: Appendixes E and F -- Risk assessment information

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-06-01

This report presents the findings of an investigation into contamination of the Clinch River and Poplar Creek near the US Department of Energy`s (DOE`s) Oak Ridge Reservation (ORR) in eastern Tennessee. For more than 50 years, various hazardous and radioactive substances have been released to the environment as a result of operations and waste management activities at the ORR. In 1989, the ORR was placed on the National Priorities List (NPL), established and maintained under the federal Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Under CERCLA, NPL sites must be investigated to determine the nature and extent of contamination at the site, assess the risk to human health and the environment posed by the site, and, if necessary, identify feasible remedial alternatives that could be used to clean the site and reduce risk. To facilitate the overall environmental restoration effort at the ORR, CERCLA activities are being implemented individually as distinct operable units (OUs). This document is the combined Remedial Investigation and Feasibility Study Report for the Clinch River/Poplar Creek OU.

Data management for the Clinch River Breeder Reactor Plant Project by use of document status and hold systems

International Nuclear Information System (INIS)

Hunt, C.S.; Beck, A.E.; Akhtar, M.S.

1982-01-01

This paper describes the development, framework, and scope of the Document Status System and the Document Hold System for the Clinch River Breeder Reactor Plant Project. It shows how data are generated at five locations and transmitted to a central computer for processing and storage. The resulting computerized data bank provides reports needed to perform day-to-day management and engineering planning. Those reports also partially satisfy the requirements of the Project's Quality Assurance Program

Conducta alimentaria de Daphnia ambigua Scourfield 1947, Moina micrura Kurz 1874 y Ceriodaphnia dubia Richard 1895 (Cladocera frente a un gradiente de concentración de alimento Feeding behaviour of Daphnia ambigua Scourfield 1947, Moina micrura Kurz 1874 and Ceriodaphnia dubia Richard 1895 (Cladocera under a food concentration gradient

Directory of Open Access Journals (Sweden)

GINGER MARTINEZ

2000-03-01

Full Text Available Debido a que Cladocera es el grupo de microfiltradores más abundante del zooplancton límnico y cuyas poblaciones se encuentran usualmente limitadas por alimento, la respuesta de los individuos frente a un gradiente de concentración de recursos tendría significativas implicancias sobre los patrones poblacionales y comunitarios. Se comparó la conducta alimentaria de Moina micrura, Ceriodaphnia dubia y Daphnia ambigua (Cladocera frente a las microalgas Chlorella sp. y Oocystis sp. (Chlorophyceae mediante los siguientes parámetros: Tasa de Ingesta Máxima (Imax, Concentración Limitante Incipiente (CLI y Eficiencia de Consumo (b, los cuales fueron obtenidos a través de un modelo de respuesta funcional. Los resultados mostraron que D. ambigua presentó las mayores Imax y CLI sobre ambos recursos, sin embargo, esta especie presentó la menor b. Mientras que D. ambigua no presentó diferencias en b sobre Chlorella sp. y Oocystis sp., M. micrura presentó una mayor b sobre Chlorella sp. y C. dubia sobre Oocystis sp. Estos resultados revelan significativas diferencias interespecíficas en la respuesta alimentaria de Cladocera, grupo que presentó un complejo comportamiento frente a variaciones en la disponibilidad de recursos alimentariosBecause Cladocera is the most abundant group of filter-feeders in the freshwater zooplankton and their populations are usually found under limited resources conditions, the individual response under a resources concentration gradient has important consequences on the population and community patterns. Feeding behavior of Moina micrura, Ceriodaphnia dubia and Daphnia ambigua (Cladocera on the microalgae Chlorella sp. and Oocystis sp. (Chlorophyceae was compared by using the following parameters: Maximum Ingestion Rate (Imax, Incipient Limiting Concentration (ILC and Consumption Efficiency (b, which were obtained through a model of functional response. Results indicated that D. ambigua showed the highest Imax and

Internal fluid flow management analysis for Clinch River Breeder Reactor Plant sodium pumps

International Nuclear Information System (INIS)

Cho, S.M.; Zury, H.L.; Cook, M.E.; Fair, C.E.

1978-12-01

The Clinch River Breeder Reactor Plant (CRBRP) sodium pumps are currently being designed and the prototype unit is being fabricated. In the design of these large-scale pumps for elevated temperature Liquid Metal Fast Breeder Reactor (LMFBR) service, one major design consideration is the response of the critical parts to severe thermal transients. A detailed internal fluid flow distribution analysis has been performed using a computer code HAFMAT, which solves a network of fluid flow paths. The results of the analytical approach are then compared to the test data obtained on a half-scale pump model which was tested in water. The details are presented of pump internal hydraulic analysis, and test and evaluation of the half-scale model test results

Observed and predicted reproduction of Ceriodaphnia dubia exposed to chloride, sulfate, and bicarbonate

Science.gov (United States)

Lasier, Peter J.; Hardin, Ian R.

2010-01-01

Chronic toxicities of Cl-, SO42-, and HCO3- to Ceriodaphnia dubia were evaluated in low- and moderate-hardness waters using a three-brood reproduction test method. Toxicity tests of anion mixtures were used to determine interaction effects and to produce models predicting C. dubia reproduction. Effluents diluted with low- and moderate-hardness waters were tested with animals acclimated to low- and moderate-hardness conditions to evaluate the models and to assess the effects of hardness and acclimation. Sulfate was significantly less toxic than Cl- and HCO3- in both types of water. Chloride and HCO3- toxicities were similar in low-hardness water, but HCO3- was the most toxic in moderate-hardness water. Low acute-to-chronic ratios indicate that toxicities of these anions will decrease quickly with dilution. Hardness significantly reduced Cl- and SO42- toxicity but had little effect on HCO3-. Chloride toxicity decreased with an increase in Na+ concentration, and CO3- toxicity may have been reduced by the dissolved organic carbon in effluent. Multivariate models using measured anion concentrations in effluents with low to moderate hardness levels provided fairly accurate predictions of reproduction. Determinations of toxicity for several effluents differed significantly depending on the hardness of the dilution water and the hardness of the water used to culture test animals. These results can be used to predict the contribution of elevated anion concentrations to the chronic toxicity of effluents; to identify effluents that are toxic due to contaminants other than Cl-, SO42-, and HCO3-; and to provide a basis for chemical substitutions in manufacturing processes.

Blaptica dubia as sentinels for exposure to chemical warfare agents - a pilot study.

Science.gov (United States)

Worek, Franz; Seeger, Thomas; Neumaier, Katharina; Wille, Timo; Thiermann, Horst

2016-11-16

The increased interest of terrorist groups in toxic chemicals and chemical warfare agents presents a continuing threat to our societies. Early warning and detection is a key component for effective countermeasures against such deadly agents. Presently available and near term solutions have a number of major drawbacks, e.g. lack of automated, remote warning and detection of primarily low volatile chemical warfare agents. An alternative approach is the use of animals as sentinels for exposure to toxic chemicals. To overcome disadvantages of vertebrates the present pilot study was initiated to investigate the suitability of South American cockroaches (Blaptica dubia) as warning system for exposure to chemical warfare nerve and blister agents. Initial in vitro experiments with nerve agents showed an increasing inhibitory potency in the order tabun - cyclosarin - sarin - soman - VX of cockroach cholinesterase. Exposure of cockroaches to chemical warfare agents resulted in clearly visible and reproducible reactions, the onset being dependent on the agent and dose. With nerve agents the onset was related to the volatility of the agents. The blister agent lewisite induced signs largely comparable to those of nerve agents while sulfur mustard exposed animals exhibited a different sequence of events. In conclusion, this first pilot study indicates that Blaptica dubia could serve as a warning system to exposure of chemical warfare agents. A cockroach-based system will not detect or identify a particular chemical warfare agent but could trigger further actions, e.g. specific detection and increased protective status. By designing appropriate boxes with (IR) motion sensors and remote control (IR) camera automated off-site warning systems could be realized. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Avaliação da atividade antagonista in vitro de fungos endofíticos associados ao camu-camu (Myrciaria dubia

Directory of Open Access Journals (Sweden)

Vanessa Carolina de Sena Correia

2015-12-01

Full Text Available Fungos endofíticos são aqueles que habitam o interior dos tecidos de seus hospedeiros, desempenhando variadas e estreitas relações ecológicas sem demonstrar sintomas visíveis. Estima-se que a maioria das espécies vegetais possuam micro-organismos endofíticos ainda não classificados e com propriedades pouco conhecidas, mas potencialmente aptos para alguma aplicação biotecnológica. O camu-camu (Myrciaria dubia apresenta grande potencial econômico, pelas características nutricionais do seu fruto, como a alta produção de vitamina C. Foram isolados e cultivados 307 fungos endofíticos associados às folhas e caules de M. dubia, a fim de verificar a atividade antagonista, quanto à produção de substâncias difusíveis e voláteis contra os seguintes fitopatógenos: Monilinia fructicola, Colletotrichum gloeosporioides e Aspergillus parasiticus. Verificou-se que 22 isolados inibiram o crescimento de pelo menos um dos fitopatógenos alvos, apresentando potencial de inibição (PI entre 25,62% a 93,84%, sendo que cinco endofíticos se destacaram, com porcentual de inibição acima de 50%, através da produção de substâncias difusíveis e voláteis. As interações competitivas foram analisadas segundo a escala de Badalyan, e observou-se maior frequência entre os fungos, da interação do tipo A (“deadlock” com contato micelial. Conclui-se que fungos endofíticos isolados de folhas e caules de Myrciaria dubia, possuem potencial biotecnológico promissor no biocontrole dos fitopatógenos (A.parasiticus, M. fructicola e C. gloeosporioides.

Morfologia dos imaturos e ciclo evolutivo de Anacassis dubia (Boheman, A. fuscata (Klug, A. languida (Boheman, A. phaeopoda Buzzi e A. punctulata (Klug (Coleoptera, Chrysomelidae, Cassidinae Morphology of the imatures and life cycle of Anacassis dubia (Boheman, A. fuscata (Klug, A. languida (Boheman, A. phaeopoda Buzzi and A. punctulata (Klug (Coleoptera, Chrysomelidae, Cassidinae

Directory of Open Access Journals (Sweden)

Zundir José Buzzi

1996-01-01

Full Text Available Eggs, fifth instar larvae and pupae of A. dubia (Boheman, 1854, A. fuscata (Klug, 1829, A. languida (Boheman, 1854, A. phaeopoda Buzzi, 1976 and A. punctulata (Klug, 1829 are described. Imatures stages were reared in a stove Biometron under temperature of 25±2ºC, photophase of 12 hours and relative humidity of 80±5%. The larvae of A. dubia and A. languida were fed with leaves of Baccharis relusa DC; A. fuscata with leaves of Baccharidastrum argutum (Less Cabrera; A. phaeopoda with leaves of Baccharis gaudichaudiana DC. and larvae of A. punctulata with leaves of Baccharis trimera (Less A. DC.. All plants belonging to the Asteraceae. Two forms of A. fuscata were studied: A. fuscata (Klug, 1829 and A. fuscata form=unicolor (Burmeister, 1870 n.syn. and no significant differences were found.

Aldose reductase inhibitors from the leaves of Myrciaria dubia (H. B. & K.) McVaugh.

Science.gov (United States)

Ueda, H; Kuroiwa, E; Tachibana, Y; Kawanishi, K; Ayala, F; Moriyasu, M

2004-11-01

Ellagic acid (1) and its two derivatives, 4-O-methylellagic acid (2) and 4-(alpha-rhamnopyranosyl)ellagic acid (3) were isolated as inhibitors of aldose reductase (AR) from Myrciaria dubia (H. B. & K.) McVaugh. Compound 2 was the first isolated from the nature. Compound 3 showed the strongest inhibition against human recombinant AR (HRAR) and rat lens AR (RLAR). Inhibitory activity of compound 3 against HRAR (IC50 value = 4.1 x 10(-8) M) was 60 times more than that of quercetin (2.5 x 10(-6) M). The type of inhibition against HRAR was uncompetitive.

Variación del contenido de vitamina C y antocianinas en Myrciaria dubia "camu camu"

OpenAIRE

Castro Gómez, Juan C.; Gutiérrez Rodríguez, Freddy; Acuña Amaral, Cinthya; Cerdeira Gutiérrez, Luis A.; Tapullima Pacaya, Alex; Cobos Ruiz, Marianela; Imán Correa, Sixto A.

2013-01-01

Myrciaria dubia es un arbusto amazónico que produce varios compuestos nutritivos y bioactivos. Esta investigación se realizó para ampliar el conocimiento relacionado a la vitamina C y antocianinas. Las muestras fueron obtenidas de la Colección de Germoplasma del INIA. La vitamina C y las antocianinas se analizaron con técnicas estándares. El contenido de vitamina C mostró una amplia variación y gradientes de concentración con diferencias significativas en frutos verdes (F = 36, gl = 3, p < 0,...

Effects of depleted uranium on the health and survival of Ceriodaphnia dubia and Hyalella azteca

Science.gov (United States)

Kuhne, W.W.; Caldwell, C.A.; Gould, W.R.; Fresquez, P.R.; Finger, S.

2002-01-01

Depleted uranium (DU) has been used as a substitute for the fissionable enriched uranium component of atomic weapons tested at Los Alamos National Laboratory (LANL) (Los Alamos, NM, USA) since the early 1950s, resulting in considerable concentrations of DU in the soils within the test sites. Although the movement of DU into major aquatic systems has been shown to be minimal, there are many small-order ephemeral streams and areas of standing water in canyons throughout LANL that may be affected by inputs of DU via runoff, erosion, and leaching. Ninety-six-hour acute and 7-d chronic toxicity assays were conducted to measure the toxicity of DU on survival and reproduction of Ceriodaphnia dubia. A 14-d water-only assay was conducted to measure survival and growth of Hyalella azteca. The estimated median lethal concentration (LC50) to produce 50% mortality of the test population for the 96-h Ceriodaphnia dubia assay was 10.50 mg/L. Reproductive effects occurred at a lowest-observable-effect concentration ???3.91 mg/L with a no-observable-effect concentration of 1.97 mg/L. The estimated 14-d LC50 for the Hyalella azteca assay was 1.52 mg/L No significant relationship was detected between growth and DU concentrations. Concentrations at which toxicity effects were observed in this study for both invertebrates exceeded concentrations of total uranium observed in runoff from LANL lands. Thus, it is likely that current runoff levels of uranium do not pose a threat to these types of aquatic invertebrates.

Individual and binary toxicity of anatase and rutile nanoparticles towards Ceriodaphnia dubia

International Nuclear Information System (INIS)

Iswarya, V.; Bhuvaneshwari, M.; Chandrasekaran, N.; Mukherjee, Amitava

2016-01-01

Highlights: • Individual, binary toxicity of anatase and rutile NPs studied on Ceriodaphnia dubia. • Anatase and rutile phases showed differential effect upon variation in irradiation. • Mixture induced antagonistic at visible and additive effect at UV-A irradiation. • Marking-Dawson model fitted more appropriately than Abbott model. • Agglomeration played a major role in the toxicity induced by the mixture. - Abstract: Increasing usage of engineered nanoparticles, especially Titanium dioxide (TiO_2) in various commercial products has necessitated their toxicity evaluation and risk assessment, especially in the aquatic ecosystem. In the present study, a comprehensive toxicity assessment of anatase and rutile NPs (individual as well as a binary mixture) has been carried out in a freshwater matrix on Ceriodaphnia dubia under different irradiation conditions viz., visible and UV-A. Anatase and rutile NPs produced an LC_5_0 of about 37.04 and 48 mg/L, respectively, under visible irradiation. However, lesser LC_5_0 values of about 22.56 (anatase) and 23.76 (rutile) mg/L were noted under UV-A irradiation. A toxic unit (TU) approach was followed to determine the concentrations of binary mixtures of anatase and rutile. The binary mixture resulted in an antagonistic and additive effect under visible and UV-A irradiation, respectively. Among the two different modeling approaches used in the study, Marking-Dawson model was noted to be a more appropriate model than Abbott model for the toxicity evaluation of binary mixtures. The agglomeration of NPs played a significant role in the induction of antagonistic and additive effects by the mixture based on the irradiation applied. TEM and zeta potential analysis confirmed the surface interactions between anatase and rutile NPs in the mixture. Maximum uptake was noticed at 0.25 total TU of the binary mixture under visible irradiation and 1 TU of anatase NPs for UV-A irradiation. Individual NPs showed highest uptake under

Individual and binary toxicity of anatase and rutile nanoparticles towards Ceriodaphnia dubia

Energy Technology Data Exchange (ETDEWEB)

Iswarya, V.; Bhuvaneshwari, M.; Chandrasekaran, N.; Mukherjee, Amitava, E-mail: amit.mookerjea@gmail.com

2016-09-15

Highlights: • Individual, binary toxicity of anatase and rutile NPs studied on Ceriodaphnia dubia. • Anatase and rutile phases showed differential effect upon variation in irradiation. • Mixture induced antagonistic at visible and additive effect at UV-A irradiation. • Marking-Dawson model fitted more appropriately than Abbott model. • Agglomeration played a major role in the toxicity induced by the mixture. - Abstract: Increasing usage of engineered nanoparticles, especially Titanium dioxide (TiO{sub 2}) in various commercial products has necessitated their toxicity evaluation and risk assessment, especially in the aquatic ecosystem. In the present study, a comprehensive toxicity assessment of anatase and rutile NPs (individual as well as a binary mixture) has been carried out in a freshwater matrix on Ceriodaphnia dubia under different irradiation conditions viz., visible and UV-A. Anatase and rutile NPs produced an LC{sub 50} of about 37.04 and 48 mg/L, respectively, under visible irradiation. However, lesser LC{sub 50} values of about 22.56 (anatase) and 23.76 (rutile) mg/L were noted under UV-A irradiation. A toxic unit (TU) approach was followed to determine the concentrations of binary mixtures of anatase and rutile. The binary mixture resulted in an antagonistic and additive effect under visible and UV-A irradiation, respectively. Among the two different modeling approaches used in the study, Marking-Dawson model was noted to be a more appropriate model than Abbott model for the toxicity evaluation of binary mixtures. The agglomeration of NPs played a significant role in the induction of antagonistic and additive effects by the mixture based on the irradiation applied. TEM and zeta potential analysis confirmed the surface interactions between anatase and rutile NPs in the mixture. Maximum uptake was noticed at 0.25 total TU of the binary mixture under visible irradiation and 1 TU of anatase NPs for UV-A irradiation. Individual NPs showed highest

Remedial investigation/feasibility study of the Clinch River/Poplar Creek Operable Unit. Volume 5. Appendixes J, K, L, M, and N-other supporting information

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-06-01

This report presents the findings of an investigation into contamination of the Clinch River and Poplar Creek near the U.S. Department of Energy`s (DOE`s) Oak Ridge Reservation (ORR) in eastern Tennessee. For more than 50 years, various hazardous and radioactive substances have been released to the environment as a result of operations and waste management activities at the ORR. In 1989, the ORR was placed on the National Priorities List (NPL), established and maintained under the federal Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Under CERCLA, NPL sites must be investigated to determine the nature and extent of contamination at the site, assess the risk to human health and the environment posed by the site, and, if necessary, identify feasible remedial alternatives that could be used to clean the site and reduce risk. To facilitate the overall environmental restoration effort at the ORR, CERCLA activities are being implemented individually as distinct operable units (OUs). This document is Volume 5 of the combined Remedial Investigation and Feasibility Study Report for the Clinch River/Poplar Creek OU.

Lessons learned from the licensing process for the Clinch River Breeder Reactor Plant

International Nuclear Information System (INIS)

Dickson, P.W.; Clare, G.H.

1991-01-01

This paper presents the experience of licensing a specific liquid-metal fast breeder reactor (LMFBR), the Clinch River Breader Reactor Plant (CRBRP). It was a success story in that the licensing process was accomplished in a very short time span. The actions of the applicant and the actions of the US Nuclear Regulatory Commission (NRC) in response are presented and discussed to provide guidance to future efforts to license unconventional reactors. The history is told from the perspective of the authors. As such, some of the reasons given for success or lack of success are subjective interpretations. Nevertheless, the authors' positions provided them an excellent viewpoint to make these judgements. During the second phase of the licensing process, they were the CRBRP Technical Director and the Licensing Manager, respectively, for the Westinghouse Electric Corporation, the prime contractor for the reactor plant

De novo assembly and functional annotation of Myrciaria dubia fruit transcriptome reveals multiple metabolic pathways for L-ascorbic acid biosynthesis.

Science.gov (United States)

Castro, Juan C; Maddox, J Dylan; Cobos, Marianela; Requena, David; Zimic, Mirko; Bombarely, Aureliano; Imán, Sixto A; Cerdeira, Luis A; Medina, Andersson E

2015-11-24

Myrciaria dubia is an Amazonian fruit shrub that produces numerous bioactive phytochemicals, but is best known by its high L-ascorbic acid (AsA) content in fruits. Pronounced variation in AsA content has been observed both within and among individuals, but the genetic factors responsible for this variation are largely unknown. The goals of this research, therefore, were to assemble, characterize, and annotate the fruit transcriptome of M. dubia in order to reconstruct metabolic pathways and determine if multiple pathways contribute to AsA biosynthesis. In total 24,551,882 high-quality sequence reads were de novo assembled into 70,048 unigenes (mean length = 1150 bp, N50 = 1775 bp). Assembled sequences were annotated using BLASTX against public databases such as TAIR, GR-protein, FB, MGI, RGD, ZFIN, SGN, WB, TIGR_CMR, and JCVI-CMR with 75.2 % of unigenes having annotations. Of the three core GO annotation categories, biological processes comprised 53.6 % of the total assigned annotations, whereas cellular components and molecular functions comprised 23.3 and 23.1 %, respectively. Based on the KEGG pathway assignment of the functionally annotated transcripts, five metabolic pathways for AsA biosynthesis were identified: animal-like pathway, myo-inositol pathway, L-gulose pathway, D-mannose/L-galactose pathway, and uronic acid pathway. All transcripts coding enzymes involved in the ascorbate-glutathione cycle were also identified. Finally, we used the assembly to identified 6314 genic microsatellites and 23,481 high quality SNPs. This study describes the first next-generation sequencing effort and transcriptome annotation of a non-model Amazonian plant that is relevant for AsA production and other bioactive phytochemicals. Genes encoding key enzymes were successfully identified and metabolic pathways involved in biosynthesis of AsA, anthocyanins, and other metabolic pathways have been reconstructed. The identification of these genes and pathways is in agreement with

Remedial investigation/feasibility study of the Clinch River/Poplar Creek Operable Unit. Volume 2. Biota and representative concentrations of contaminants. Appendixes A, B, C, D

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-03-01

This report presents the findings of an investigation into contamination of the Clinch River and Poplar Creek near the U.S. Department of Energy`s (DOE`s) Oak Ridge Reservation (ORR) in eastern Tennessee. For more than 50 years, various hazardous and radioactive substances have been released to the environment as a result of operations and waste management activities at the ORR. In 1989, the ORR was placed on the National Priorities List (NPL), established and maintained under the federal Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Under CERCLA, NPL sites must be investigated to determine the nature and extent of contamination at the site, assess the risk to human health and the environment posed by the site, and, if necessary, identify feasible remedial alternatives that could be used to clean the site and reduce risk. To facilitate the overall environmental restoration effort at the ORR, CERCLA activities are being implemented individually as distinct operable units (OU`s). This document is the combined Remedial Investigation and Feasibility Study Report for the Clinch River/Poplar Creek OU.

Antimicrobial constituents of peel and seeds of camu-camu (Myrciaria dubia).

Science.gov (United States)

Kaneshima, Tai; Myoda, Takao; Toeda, Kazuki; Fujimori, Takane; Nishizawa, Makoto

2017-08-01

Various antimicrobial constituents of camu-camu fruit were isolated. Acylphloroglucinol (compound 1) and rhodomyrtone (compound 2) were isolated from the peel of camu-camu (Myrciaria dubia) fruit, while two other acylphloroglucinols (compounds 3 and 4) were obtained from camu-camu seeds. The structures of the isolated compounds were characterized by spectrophotometric methods. Compounds 1 and 4 were confirmed to be new acylphloroglucinols with different substituents at the C7 or C9 position of 2, and were named myrciarone A and B, respectively. Compound 3 was determined to be isomyrtucommulone B. This is the first report of the isolation of 3 from a natural resource. The antimicrobial activities of compounds 1, 3, and 4 were similar to those of 2, and the minimum inhibitory concentrations were either similar to or lower than that of kanamycin. These results suggest that the peel and seeds of camu-camu fruit could be utilized for therapeutic applications.

Clonación molecular de los genes GDP-L-galactosa fosforilasa, L-galactosa deshidrogenasa y L-galactono-1.4-lactona deshidrogenasa de la vía biosintética de vitamina C en Myrciaria dubia (camu camu)

OpenAIRE

Egoávil, Alina; Universidad Nacional de la Amazonía Peruana; Torres, Julián; Cobos, Marianela; Universidad Nacional de la Amazonía Peruana; Imán, Sixto A.; Universidad Nacional de la Amazonía Peruana; Marapara, Jorge L.; Universidad Nacional de la Amazonía Peruana; Castro, Juan C.; Universidad Nacional de la Amazonía Peruana

2016-01-01

El objetivo del estudio fue realizar la clonación molecular de los genes que codifican las enzimas, GDP-L-galactosa fosforilasa (GGF), L-galactosa deshidrogenasa (GDH) y L-galactono-1,4-lactona deshidrogenasa (GLDH) de la ruta biosintética de vitamina C de Myrciaria dubia (Kunth) McVaugh. Los frutos fueron obtenidos de la Colección Nacional de Germoplasma de M. dubia del Instituto Nacional de Innovación Agraria. El ARN se purificó, se sintetizó el ADNc y amplificó con cebadores degenerados, s...

Remedial investigation/feasibility study of the Clinch River/Poplar Creek operable unit. Volume 4. Information related to the feasibility study and ARARs. Appendixes G, H, I

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-03-01

This report presents the findings of an investigation into contamination of the Clinch River and Poplar Creek near the U.S. Department of Energy`s (DOE`s) Oak Ridge Reservation (ORR) in eastern Tennessee. For more than 50 years, various hazardous and radioactive substances have been released to the environment as a result of operations and waste management activities at the ORR. In 1989, the ORR was placed on the National Priorities List (NPL), established and maintained under the federal Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Under CERCLA, NPL sites must be investigated to determine the nature and extent of contamination at the site, assess the risk to human health and the environment posed by the site, and, if necessary, identify feasible remedial alternatives that could be used to clean the site and reduce risk. To facilitate the overall environmental restoration effort at the ORR, CERCLA activities are being implemented individually as distinct operable units (OUs). This document is the combined Remedial Investigation and Feasibility Study Report for the Clinch River/Poplar Creek OU.

Remedial investigation/feasibility study of the Clinch River/Poplar Creek Operable Unit. Volume 2. Appendixes A, B, C, and D-Biota and representative concentrations of contaminants

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-06-01

This report presents the findings of an investigation into contamination of the Clinch River and Poplar Creek near the U.S. Department of Energy`s (DOE`s) Oak Ridge Reservation (ORR) in eastern Tennessee. For more than 50 years, various hazardous and radioactive substances have been released to the environment as a result of operations and waste management activities at the ORR. In 1989, the ORR was placed on the National Priorities List (NPL), established and maintained under the federal Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Under CERCLA, NPL sites must be investigated to determine the nature and extent of contamination at the site, assess the risk to human health and the environment posed by the site, and, if necessary, identify feasible remedial alternatives that could be used to clean the site and reduce risk. To facilitate the overall environmental restoration effort at the ORR, CERCLA activities are being implemented individually as distinct operable units (OUs). This document is Volume 2 of the combined Remedial Investigation and Feasibility Study Report for the Clinch River/Poplar Creek OU.

Les cochenilles des Myrtaceae en Amazonie péruvienne : 1. Description de deux nouveaux ravageurs de Myrciaria dubia (Homoptera, Coccidae et Kerriidae)

OpenAIRE

Matile-Ferrero, D.; Couturier, Guy

1993-01-01

#Ceroplastes flosculoides$ Matile-Ferrero n.sp. (Coccidae) et #Austrotachardiella sexcordata$ Matile-Ferrero n.sp. (Kerriidae), sont décrits et illustrés. Les deux espèces sont des ravageurs d'une Myrtacée cultivée, #Myrciaria dubia$, en Amazonie péruvienne. #Austrotachardiella trilobata$ (Mendes) n.comb. est transférée de #Tachardiella$ Cockerell. (Résumé d'auteur)

Protected air-cooled condenser for the Clinch River Breeder Reactor Plant

International Nuclear Information System (INIS)

Louison, R.; Boardman, C.E.

1981-01-01

The long term residual heat removal for the Clinch River Breeder Reactor Plant (CRBRP) is accomplished through the use of three protected air-cooled condensers (PACC's) each rated at 15M/sub t/ following a normal or emergency shutdown of the reactor. Steam is condensed by forcing air over the finned and coiled condenser tubes located above the steam drums. The steam flow is by natural convection. It is drawn to the PACC tube bundle for the steam drum by the lower pressure region in the tube bundle created from the condensing action. The concept of the tube bundle employs a unique patented configuration which has been commercially available through CONSECO Inc. of Medfore, Wisconsin. The concept provides semi-parallel flow that minimizes subcooling and reduces steam/condensate flow instabilities that have been observed on other similar heat transfer equipment such as moisture separator reheaters (MSRS). The improved flow stability will reduce temperature cycling and associated mechanical fatigue. The PACC is being designed to operate during and following the design basis earthquake, depressurization from the design basis tornado and is housed in protective building enclosure which is also designed to withstand the above mentioned events

The Clinch River Breeder Reactor Plant: an analysis of the impacts of its in-migrant construction workers on local public services. Final report

International Nuclear Information System (INIS)

Braid, R.B. Jr.; Kyles, S.D.

1977-05-01

The socioeconomic impact study identifies certain impacts which are projected to occur to local public services in each of 14 Tennessee communities in the Oak Ridge-Knoxville area during the construction of the Clinch River Breeder Reactor Plant. Various in-migration scenarios are utilized, and detailed qualitative and quantitative analyses of each public service are undertaken. Per capita in-migrant cost-revenue impacts are calculated for each community in each in-migration scenario

Remedial investigation/feasibility study of the Clinch River/Poplar Creek Operable Unit. Volume 4. Appendixes G, H, and I and information related to the feasibility study and ARARs

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-06-01

This report presents the findings of an investigation into contamination of the Clinch River and Poplar Creek near the U.S. Department of Energy`s (DOE`s) Oak Ridge Reservation (ORR) in eastern Tennessee. For more than 50 years, various hazardous and radioactive substances have been released to the environment as a result of operations and waste management activities at the ORR. In 1989, the ORR was placed on the National Priorities List (NPL), established and maintained under the federal Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Under CERCLA, NPL sites must be investigated to determine the nature and extent of contamination at the site, assess the risk to human health and the environment posed by the site, and, if necessary, identify feasible remedial alternatives that could be used to clean the site and reduce risk. To facilitate the overall environmental restoration effort at the ORR, CERCLA activities are being implemented individually as distinct operable units (OUs). This document is Volume 4 of the combined Remedial Investigation and Feasibility Study Report for the Clinch River/Poplar Creek OU.

La expresión y actividad catalítica diferencial de GDP-manosa pirofosforilasa influye en la producción de vitamina C en Myrciaria dubia “camu-camu”

Directory of Open Access Journals (Sweden)

Juan Castro Gómez

2013-12-01

Full Text Available Myrciaria dubia muestra una amplia variación en la producción de vitamina C (vit.C. Para entender las bases moleculares de esta variación, se determinó la relación del nivel de expresión genética y actividad de GDP-Manosa pirofosforilasa (GMP con la variación del contenido de vit.C en M. dubia. Las muestras fueron obtenidas de una colección de germoplasma. La vit.C se cuantificó por HPLC, la actividad de GMP se midió por espectrofotometría. El ARN se purificó, el ADNc se sintetizó, amplificó, clonó y secuenció con técnicas estándares. La expresión del gen se midió mediante PCR en tiempo real. Las diferencias en el contenido de vit.C de las hojas, pulpa y cáscara dependieron de la expresión y actividad de GMP. Esta relación también se observó entre plantas que producen frutos con bajo y alto contenido de vit.C. En conclusión, Las hojas, pulpa y cáscara de M. dubia muestran diferencias significativas en el contenido de vit.C, el cual se atribuye a la expresión y actividad catalítica diferencial de la GMP. Asimismo, las plantas que producen frutos con diferencias marcadas en el contenido de vit. C en sus pulpas, también dependen del nivel de expresión y actividad catalítica de esta enzima.

La expresión y actividad catalítica diferencial de GDP-manosa pirofosforilasa influye en la producción de vitamina C en Myrciaria dubia “camu-camu”

Directory of Open Access Journals (Sweden)

Juan Castro Gómez

2013-12-01

Full Text Available Myrciaria dubia muestra una amplia variación en la producción de vitamina C (vit.C. Para entender las bases moleculares de esta variación, se determinó la relación del nivel de expresión genética y actividad de GDP-Manosa pirofosforilasa (GMP con la variación del contenido de vit.C en M. dubia. Las muestras fueron obtenidas de una colección de germoplasma. La vit.C se cuantificó por HPLC, la actividad de GMP se midió por  espectrofotometría. El ARN se purificó, el ADNc se sintetizó, amplificó, clonó y secuenció con técnicas estándares. La expresión del gen se midió mediante PCR en tiempo real. Las diferencias en el contenido de vit.C de las hojas, pulpa y cáscara dependieron de la expresión y actividad de GMP. Esta relación también se observó entre plantas que producen frutos con bajo y alto contenido de vit.C. En conclusión, Las hojas, pulpa y cáscara de M. dubia muestran diferencias significativas en el contenido de vit.C, el cual se atribuye a la expresión y actividad catalítica diferencial de la GMP. Asimismo, las plantas que producen frutos con diferencias marcadas en el contenido de vit.C en sus pulpas, también dependen del nivel de expresión y actividad catalítica de esta enzima.

Seed dormancy and germination in Jeffersonia dubia (Berberidaceae) as affected by temperature and gibberellic acid.

Science.gov (United States)

Rhie, Y H; Lee, S Y; Kim, K S

2015-03-01

The genus Jeffersonia, which contains only two species, has a trans-Atlantic disjunct distribution. The aims of this study were to determine the requirements for breaking dormancy and germination of J. dubia seeds and to compare its dormancy characteristics with those of the congener in eastern North America. Ripe seeds of J. dubia contain an underdeveloped embryo and were permeable to water. In nature, seeds were dispersed in May, while embryos began to grow in September, and were fully elongated by late November. Germination started in March of the next year, and seeds emerged as seedlings soon after germination. In laboratory experiments, incubation at high temperatures (25 °C, 25/15 °C) for at least 8 weeks was required to initiate embryo growth, while a transfer to moderate temperatures (20/10 °C, 15/6 °C) was needed for the completion of embryo growth. At least 8 weeks at 5 °C was effective in overcoming physiological dormancy and for germination in seeds after the embryos had fully elongated. Thus, both high and low temperatures were essential to break dormancy. Gibberellic acid (GA3 ) treatment could substitute for the high temperature requirement, but not for the low temperature requirement. Based on the dormancy-breaking requirements, it is confirmed that the seeds have deep simple morphophysiological dormancy. This dormancy type is similar to that of seeds of the eastern North American species J. diphylla. Although seeds require 10-11 months from seed dispersal to germination in nature, under controlled conditions they required only 3 months after treatment with 1000 mg·l(-1) GA3 , followed by incubation at 15/6 °C. This represents practical knowledge for propagation of these plants from seed. © 2014 German Botanical Society and The Royal Botanical Society of the Netherlands.

Toxicity of waters from the Rochester Embayment Area of Concern to the plankton species Pseudokirchneriella subcapitata and Ceriodaphnia dubia

Science.gov (United States)

Baldigo, Barry P.; Duffy, Brian T.; Smith, Alexander J.; George, Scott D.

2016-01-01

The lower Genesee River and Rochester Embayment of Lake Ontario are a designated Area of Concern (AOC) under the binational Great Lakes Water Quality Agreement. The “degradation of phytoplankton and zooplankton populations” or plankton Beneficial Use Impairment (BUI) was classified as unknown and in need of further assessment in this AOC because water quality data suggested plankton communities could be effected and community data were either unavailable or indicated impacts. The plankton BUI may now be obsolete because local contaminant sources have been largely eliminated. The present study was conducted between July 2013 and August 2014 to assess the BUI-removal criteria: “AOC plankton bioassays confirm that toxicity in ambient waters (i.e., no growth inhibition) is not significantly higher than comparable non-AOC controls”. Acute and chronic toxicity of waters from 13 sites were quantified seasonally using standardized bioassays with the green alga Pseudokirchneriella subcapitata and water flea Ceriodaphnia dubia to test the hypothesis that toxicity of waters from AOC sites was not higher than that of waters from comparable non-AOC reference sites. Survival and reproduction of C. dubia did not differ significantly between site types, systems, or months. The growth of P. subcapitata did not differ between site types, but differed among systems and months. All results indicate that waters from AOC sites were no more toxic to both plankton species than waters from reference sites. Assuming test species represent natural plankton assemblages, water quality should not negatively affect survival and growth of resident plankton populations in the Rochester Embayment AOC.

Eficacia tópica de Myrciaria dubia en la curación de quemaduras de segundo grado en ratas Holtzman.

OpenAIRE

Karla Pacci-Salazar; Lizette Nureña-Noriega; José Vásquez-Cerro; Guillermo Araujo-Espinoza; Marco Gálvez-Niño

2009-01-01

Objetivo: Comparar el efecto de la crema a base de Myrciaria dubia con el efecto antibiótico de la crema de sulfadiazina argéntica. Diseño: El presente es un estudio experimental aleatorizado incompleto. Participantes: 15 ratas Holtzman hembras de 9-10 semanas a las cuales se les produjo quemaduras de segundo grado. Intervenciones: Se produjo una quemadura cuadrada de 1cm2 de piel del dorso de las ratas usando un cautín modificado para producir tres quemaduras térmicas por cada animal. Se sel...

Fluctuación diurna del contenido de vitamina C en hojas de Myrciaria dubia “camu camu”

Directory of Open Access Journals (Sweden)

Franz Correa Meléndez

2013-12-01

Full Text Available Myrciaria dubia “camu camu” es un frutal del trópico amazónico caracterizado por sus frutos con gran contenido de vitamina C, siendo considerado un producto importante del país. Sin embargo, hay pocos reportes sobre el metabolismo y transporte de vitamina C en esta especie. El objetivo de la investigación fue determinar la fluctuación diurna del contenido de vitamina C en hojas de M. dubia “camu camu”. Las hojas se colectaron de la colección de germoplasma de “camu camu” del INIA a las 2, 6, 10, 14, 18 y 22 horas; de cuatro plantas y en tres fechas diferentes. La vitamina C fue extraída de las hojas con un método estandarizado en el laboratorio y se cuantificó mediante espectrofotometría a 530nm, previa reacción con 2,6-diclorofenolindofenol. Los resultados muestran que el contenido de vitamina C de las hojas de “camu camu” fue en promedio 231±35 mg vitamina C/100g de hoja. Además, el contenido de vitamina C en las hojas del “camu camu” mostró fluctuación diurna, siendo menor a las 6 horas y mayor a las 2 y 14 horas del día. También, se registraron concentraciones intermedias en horas de menor o ausencia de radiación solar (235±17 y 237±35 mg vitamina C/100g de hoja a las 18 y 22 horas respectivamente. Se concluye que existe alto contenido de vitamina C en las hojas del “camu camu” y que este contenido presenta fluctuación diurna.

Geographic Place Names, GNIS data for Atkinson, Bacon, Ben Hill, Berrien, Brantley, Brooks, Charlton, Clinch, Coffee, Cook, Echols, Irwin, Lanier, Lowndes, Pierce, Tift, Turner, Ware counties., Published in 1999, 1:7200 (1in=600ft) scale, Southern Georgia Regional Commission.

Data.gov (United States)

NSGIC Regional | GIS Inventory — Geographic Place Names dataset current as of 1999. GNIS data for Atkinson, Bacon, Ben Hill, Berrien, Brantley, Brooks, Charlton, Clinch, Coffee, Cook, Echols, Irwin,...

Three-dimensional finite-element analysis of the cellular convection phenomena in the Clinch River Breeder Reactor Plant prototype pump

International Nuclear Information System (INIS)

Silver, A.H.; Lee, J.Y.

1983-01-01

Cellular convection was studied rigorously during the development of the Clinch River Breeder Reactor Plant (CRBRP) Program Pumps. This paper presents the development of a three-dimensional finite-element heat transfer model which accounts for the cellular convection phenomena. A buoyancy driven cellular convection flow pattern is introduced in the annulus region between the upper inner structure and the pump tank. Steady-state thermal data were obtained for several test conditions for argon gas pressures up to 93 psig (741 kPa) and sodium operating temperatures to 1000 0 F (811 0 K). Test temperature distributions on the pump tank and inner structure were correlated with numerical results and excellent agreement was obtained

Finite strain anisotropic elasto-plastic model for the simulation of the forming and testing of metal/short fiber reinforced polymer clinch joints at room temperature

Science.gov (United States)

Dean, A.; Rolfes, R.; Behrens, A.; Bouguecha, A.; Hübner, S.; Bonk, C.; Grbic, N.

2017-10-01

There is a strong trend in the automotive industry to reduce car body-, chassis- and power-train mass in order to lower carbon emissions. More wide spread use of lightweight short fiber reinforced polymer (SFRP) is a promising approach to attain this goal. This poses the challenge of how to integrate new SFRP components by joining them to traditional sheet metal structures. Recently (1), the clinching technique has been successfully applied as a suitable joining method for dissimilar material such as SFRP and Aluminum. The material pairing PA6GF30 and EN AW 5754 is chosen for this purpose due to their common application in industry. The current contribution presents a verification and validation of a finite strain anisotropic material model for SFRP developed in (2) for the FE simulation of the hybrid clinching process. The finite fiber rotation during forming and separation, and thus the change of the preferential material direction, is represented in this model. Plastic deformations in SFRP are considered in this model via an invariant based non-associated plasticity formulation following the multiplicative decomposition approach of the deformation gradient where the stress-free intermediate configuration is introduced. The model allows for six independent characterization curves. The aforementioned material model allows for a detailed simulation of the forming process as well as a simulative prediction of the shear test strength of the produced joint at room temperature.

Citoprotective effect of camu-camu Myrciaria dubia on three celular lines of mouse exposed in vivo to potassium bromate

OpenAIRE

Rafael, Alvis; Pino, José; Gonzáles, José; Francia, Juan C.; Shiga, Betty

2011-01-01

Se evaluó in vivo la capacidad citoprotectora del fruto de Myrciaria dubia (Kunth) McVaugh Camu-camu frente al daño mutagénico producido por bromato de potasio (68,5 mg/k) sobre tres líneas celulares de ratón (hígado, riñón y células sanguíneas). Se utilizó ratones (n= 120) divididos en tres grupos los cuales bebieron ad libitum: TI= control negativo (solo agua) y el grupo TIII (control positivo); El grupo TII bebió el extracto acuoso (2% p/v) del fruto de camu-camu. A los diez días se inyect...

Effects of water quality parameters on boron toxicity to Ceriodaphnia dubia.

Science.gov (United States)

Dethloff, Gail M; Stubblefield, William A; Schlekat, Christian E

2009-07-01

The potential modifying effects of certain water quality parameters (e.g., hardness, alkalinity, pH) on the acute toxicity of boron were tested using a freshwater cladoceran, Ceriodaphnia dubia. By comparison, boron acute toxicity was less affected by water quality characteristics than some metals (e.g., copper and silver). Increases in alkalinity over the range tested did not alter toxicity. Increases in water hardness appeared to have an effect with very hard waters (>500 mg/L as CaCO(3)). Decreased pH had a limited influence on boron acute toxicity in laboratory waters. Increasing chloride concentration did not provide a protective effect. Boron acute toxicity was unaffected by sodium concentrations. Median acute lethal concentrations (LC(50)) in natural water samples collected from three field sites were all greater than in reconstituted laboratory waters that matched natural waters in all respects except for dissolved organic carbon. Water effect ratios in these waters ranged from 1.4 to 1.8. In subsequent studies using a commercially available source of natural organic matter, acute toxicity decreased with increased dissolved organic carbon, suggesting, along with the natural water studies, that dissolved organic carbon should be considered further as a modifier of boron toxicity in natural waters where it exceeds 2 mg/L.

Modeling and analysis of the unprotected loss-of-flow accident in the Clinch River Breeder Reactor

International Nuclear Information System (INIS)

Morris, E.E.; Dunn, F.E.; Simms, R.; Gruber, E.E.

1985-01-01

The influence of fission-gas-driven fuel compaction on the energetics resulting from a loss-of-flow accident was estimated with the aid of the SAS3D accident analysis code. The analysis was carried out as part of the Clinch River Breeder Reactor licensing process. The TREAT tests L6, L7, and R8 were analyzed to assist in the modeling of fuel motion and the effects of plenum fission-gas release on coolant and clad dynamics. Special, conservative modeling was introduced to evaluate the effect of fission-gas pressure on the motion of the upper fuel pin segment following disruption. For the nominal sodium-void worth, fission-gas-driven fuel compaction did not adversely affect the outcome of the transient. When uncertainties in the sodium-void worth were considered, however, it was found that if fuel compaction occurs, loss-of-flow driven transient overpower phenomenology could not be precluded

Phase 1 data summary report for the Clinch River Remedial Investigation: Health risk and ecological risk screening assessment

Energy Technology Data Exchange (ETDEWEB)

Cook, R.B.; Adams, S.M.; Beauchamp, J.J.; Bevelhimer, M.S.; Blaylock, B.G.; Brandt, C.C.; Ford, C.J.; Frank, M.L.; Gentry, M.J.; Holladay, S.K.; Hook, L.A.; Levine, D.A.; Longman, R.C.; McGinn, C.W.; Skiles, J.L.; Suter, G.W.; Williams, L.F.

1992-12-01

The Clinch River Remedial Investigation (CRRI) is designed to address the transport, fate, and distribution of waterborne contaminants released from the US Department of Energy's (DOE's) Oak Ridge Reservation (ORR) and to assess potential risks to human health and the environment associated with these contaminants. The contaminants released since the early 1940s include a variety of radionuclides, metals, and organic compounds. The purpose of this report is to summarize the results of Phase 1 of the CRRI. Phase 1 was designed to (1) obtain high-quality data to confirm existing historical data for contaminant levels in fish, sediment, and water from the CR/WBR; (2) determine the in the range of contaminant concentrations present river-reservoir system; (3) identify specific contaminants of concern; and (4) establish the reference (background) concentrations for those contaminants.

Safety evaluation report related to the construction of the Clinch River Breeder Reactor Plant. Docket No. 50-537. Suppl. 1

International Nuclear Information System (INIS)

1983-05-01

Since the preparation of the Safety Evaluation Report the Advisory Committee on Reactor Safeguards considered the Clinch River construction permit license application at its 276th meeting and subsequently issued a favorable report, dated April 19, 1983 to the Commission (See Appendix I of this report). Additional documents associated with the application have been reviewed and a number of meetings have been held with the applicants. These events and documents are identified in Appendix E to this supplement. This supplement, SSER-1, to the Safety Evaluation Report, provides an evaluation of additional information received from the applicants since preparation of the SER regarding previously identified outstanding review items, and our response to the comments made by the Advisory Committee on Reactor Safeguards in its report

1-methylmalate from camu-camu (Myrciaria dubia) suppressed D-galactosamine-induced liver injury in rats.

Science.gov (United States)

Akachi, Toshiyuki; Shiina, Yasuyuki; Kawaguchi, Takumi; Kawagishi, Hirokazu; Morita, Tatsuya; Sugiyama, Kimio

2010-01-01

To evaluate the protective effects of fruit juices against D-galactosamine (GalN)-induced liver injury, lyophilized fruit juices (total 12 kinds) were fed to rats for 7 d, and then we evoked liver injury by injecting GalN. The juice of camu-camu (Myrciaria dubia) significantly suppressed GalN-induced liver injury when the magnitude of liver injury was assessed by plasma alanine aminotransferase and aspartate aminotransferase activities, although some other juices (acerola, dragon fruit, shekwasha, and star fruit) also tended to have suppressive effects. An active compound was isolated from camu-camu juice by solvent fractionation and silica gel column chromatography. The structure was determined to be 1-methylmalate. On the other hand, malate, 1,4-dimethylmalate, citrate, and tartrate had no significant effect on GalN-induced liver injury. It is suggested that 1-methylmalate might be a rather specific compound among organic acids and their derivatives in fruit juices in suppressing GalN-induced liver injury.

Determination of anthocyanins from camu-camu (Myrciaria dubia) by HPLC-PDA, HPLC-MS, and NMR.

Science.gov (United States)

Zanatta, Cinthia Fernanda; Cuevas, Elyana; Bobbio, Florinda O; Winterhalter, Peter; Mercadante, Adriana Z

2005-11-30

Camu-camu [Myrciaria dubia (HBK) McVaugh] is a small fruit native to the Amazonian rain forest. Its anthocyanin profile has now been investigated for the first time. Fruits from two different regions of the São Paulo state, Brazil, were analyzed. The major anthocyanins were isolated by high-speed countercurrent chromatography. HPLC-PDA, HPLC-MS/MS, and 1H NMR were used to confirm the identity of the main anthocyanins of camu-camu. Cyanidin-3-glucoside was identified as the major pigment in the fruits from both regions, representing 89.5% in the fruits produced in Iguape and 88.0% in those from Mirandópolis, followed by the delphinidin-3-glucoside, ranging between 4.2 and 5.1%, respectively. Higher total anthocyanin contents were detected in the fruits from Iguape (54.0 +/- 25.9 mg/100 g) compared to those from Mirandópolis (30.3 +/- 6.8 mg/100 g), most likely because of the lower temperatures in the Iguape region.

Data Management Plan and Functional System Design for the Information Management System of the Clinch River Remedial Investigation and Waste Area Grouping 6

Energy Technology Data Exchange (ETDEWEB)

Ball, T.; Brandt, C.; Calfee, J.; Garland, M.; Holladay, S.; Nickle, B.; Schmoyer, D.; Serbin, C.; Ward, M. [Oak Ridge National Lab., TN (United States)

1994-03-01

The Data Management Plan and Functional System Design supports the Clinch River Remedial Investigation (CRRI) and Waste Area Grouping (WAG) 6 Environmental Monitoring Program. The objective of the Data Management Plan and Functional System Design is to provide organization, integrity, security, traceability, and consistency of the data generated during the CRRI and WAG 6 projects. Proper organization will ensure that the data are consistent with the procedures and requirements of the projects. The Information Management Groups (IMGs) for these two programs face similar challenges and share many common objectives. By teaming together, the IMGs have expedited the development and implementation of a common information management strategy that benefits each program.

Construction and operation of Clinch River Breeder Reactor Plant, docket no. 50-537, Oak Ridge, Roane County, Tennessee

International Nuclear Information System (INIS)

Anon.

1982-01-01

Construction and operation of the Clinch River Breeder Reactor Plant (CRBRP) in Oak Ridge, Tennessee are proposed. The CRBRP would use a liquid-sodium-cooled fast-breeder reactor to produce 975 megawatts of thermal energy (MWt) with the initial core loading of uranium- and plutonium-mixed oxide fuel. This heat would be transferred by heat exchangers to nonradioactive sodium in an intermediate loop and then to a steam cycle. A steam turbine generator would use the steam to produce 380 megawatts of electrical capacity (MWe). Future core design might result in gross power ratings of 1,121 MWt and 439 MWe. Exhaust steam from the turbine generator would be cooled in condensers using two mechanical draft cooling towers. The principal benefit would be the demonstration of the LMFBR concept for commercial use. Electricity generated would be a secondary benefit. Other impacts and effects are discussed

Data on a single oral dose of camu camu (Myrciaria dubia pericarp extract on flow-mediated vasodilation and blood pressure in young adult humans

Directory of Open Access Journals (Sweden)

Tadayoshi Miyashita

2018-02-01

Full Text Available This data article describes the flow-mediated vasodilation (FMD responses, represented by changes in arterial diameter, and blood pressure changes in young adults after a single oral dose of camu camu (Myrciaria dubia pericarp extract or placebo (cross-over design. Ten healthy men and 10 healthy women participated in this study. Ultrasonic diagnostic equipment was used to monitor arterial diameter changes, indicative of FMD, for 110 s after the administration of the camu camu extract or placebo. In addition, the systolic and diastolic blood pressure values were recorded.

Data on a single oral dose of camu camu (Myrciaria dubia) pericarp extract on flow-mediated vasodilation and blood pressure in young adult humans.

Science.gov (United States)

Miyashita, Tadayoshi; Koizumi, Ryosuke; Myoda, Takao; Sagane, Yoshimasa; Niwa, Koichi; Watanabe, Toshihiro; Minami, Kazuhiro

2018-02-01

This data article describes the flow-mediated vasodilation (FMD) responses, represented by changes in arterial diameter, and blood pressure changes in young adults after a single oral dose of camu camu ( Myrciaria dubia ) pericarp extract or placebo (cross-over design). Ten healthy men and 10 healthy women participated in this study. Ultrasonic diagnostic equipment was used to monitor arterial diameter changes, indicative of FMD, for 110 s after the administration of the camu camu extract or placebo. In addition, the systolic and diastolic blood pressure values were recorded.

Comparison of oxide- and metal-core behavior during CRBRP [Clinch River Breeder Reactor Plant] station blackout

International Nuclear Information System (INIS)

Polkinghorne, S.T.; Atkinson, S.A.

1986-01-01

A resurrected concept that could significantly improve the inherently safe response of Liquid-Metal cooled Reactors (LMRs) during severe undercooling transients is the use of metallic fuel. Analytical studies have been reported on for the transient behavior of metal-fuel cores in innovative, inherently safe LMR designs. This paper reports on an analysis done, instead, for the Clinch River Breeder Reactor Plant (CRBRP) design with the only innovative change being the incorporation of a metal-fuel core. The SSC-L code was used to simulate a protected station blackout accident in the CRBRP with a 943 MWt Integral Fast Reactor (IFR) metal-fuel core. The results, compared with those for the oxide-fueled CRBRP, show that the margin to boiling is greater for the IFR core. However, the cooldown transient is more severe due to the faster thermal response time of metallic fuel. Some additional calculations to assess possible LMR design improvements (reduced primary system pressure losses, extended flow coastdown) are also discussed. 8 refs., 13 figs., 2 tabs

Remedial investigation/feasibility study for the Clinch River/Poplar Creek operable unit. Volume 5. Appendixes G, H, I, J

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-09-01

The Quality Assurance/Quality Control (QA/QC) Program for Phase 2 of the Clinch River Remedial Investigation (CRRI) was designed to comply with both Department of Energy (DOE) Order 5700.6C and Environmental Protection Agency (EPA) QAMS-005/80 (EPA 1980a) guidelines. QA requirements and the general QA objectives for Phase 2 data were defined in the Phase 2 Sampling and Analysis Plan (SAP)-Quality Assurance Project Plan, and scope changes noted in the Phase 2 Sampling and Analysis Plan Addendum. The QA objectives for Phase 2 data were the following: (1) Scientific data generated will withstand scientific and legal scrutiny. (2) Data will be gathered using appropriate procedures for sample collection, sample handling and security, chain of custody (COC), laboratory analyses, and data reporting. (3) Data will be of known precision and accuracy. (4) Data will meet data quality objectives (DQOs) defined in the Phase 2 SAP.

Supplement to Final Environmental Statement related to construction and operation of Clinch River Breeder Reactor Plant, Docket No. 50-537

International Nuclear Information System (INIS)

1982-10-01

In February 1977, the Office of Nuclear Reactor Regulation issued a Final Environmental Statement (FES) (NUREG-0139) related to the construction and operation of the proposed Clinch River Breeder Reactor Plant (CRBRP). Since the FES was issued, additional data relative to the site and its environs have been collected, several modifications have been made to the CRBRP design, and its fuel cycle, and the timing of the plant construction and operation has been affected in accordance with deferments under the DOE Liquid Metal Fast Breeder Reactor (LMFBR) program. These changes are summarized and their environmental significance is assessed in this document. The reader should note that this document generally does not repeat the substantial amount of information in the FES which is still current; hence, the FES should be consulted for a comprehensive understanding of the staff's environmental review of the CRBRP project

Phase 1 data summary report for the Clinch River Remedial Investigation: Health risk and ecological risk screening assessment. Environmental Restoration Program

Energy Technology Data Exchange (ETDEWEB)

Cook, R.B.; Adams, S.M.; Beauchamp, J.J.; Bevelhimer, M.S.; Blaylock, B.G.; Brandt, C.C.; Ford, C.J.; Frank, M.L.; Gentry, M.J.; Holladay, S.K.; Hook, L.A.; Levine, D.A.; Longman, R.C.; McGinn, C.W.; Skiles, J.L.; Suter, G.W.; Williams, L.F.

1992-12-01

The Clinch River Remedial Investigation (CRRI) is designed to address the transport, fate, and distribution of waterborne contaminants released from the US Department of Energy`s (DOE`s) Oak Ridge Reservation (ORR) and to assess potential risks to human health and the environment associated with these contaminants. The contaminants released since the early 1940s include a variety of radionuclides, metals, and organic compounds. The purpose of this report is to summarize the results of Phase 1 of the CRRI. Phase 1 was designed to (1) obtain high-quality data to confirm existing historical data for contaminant levels in fish, sediment, and water from the CR/WBR; (2) determine the in the range of contaminant concentrations present river-reservoir system; (3) identify specific contaminants of concern; and (4) establish the reference (background) concentrations for those contaminants.

Setal morphology of grooming appendages in the spider crab, Libinia dubia.

Science.gov (United States)

Wortham, Jen L; LaVelle, Amanda D

2016-08-01

In crustaceans, grooming behaviors decrease fouling by removing debris from the exoskeleton and body structures; these grooming behaviors improve respiration, sensory reception, movement, and reproduction. Setal morphologies of the following grooming appendages in the decapod crustacean spider crab Libinia dubia are examined including the first pereiopod (cheliped), first, second, and third maxillipeds (mouthparts), and first, second, and third epipods (internal extensions of the maxillipeds). The objective of this study was to describe setal morphologies of these grooming appendages and to elucidate possible functions and efficiencies of setal structures. Spider crabs are hypothesized to have elaborate setal morphologies, mainly for cleaning specialized decorating setae as well as for cleaning inside the gill chamber, which has a higher likelihood of becoming fouled compared to other decapods such as shrimps. Fourteen setal types are documented and included several varieties of serrate and pappose setae as well as simple setae, cuspidate setae, papposerrate setae, and canoe setae. Maxillipodal epipods in the gill chamber are free of fouling, suggesting the setation on the third maxilliped protopod has an efficient functional morphology in removing debris before water enters the gill chamber. Serrate setae may function for detangling and separating structures whereas pappose setae may function for fine detailed grooming. The cheliped is the only grooming appendage that can reach decorating setae and it contains only pappose setae; thus decorating setae is not likely groomed in a manner that would greatly decrease fouling. J. Morphol. 277:1045-1061, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Estudo da toxicidade de metais (zinco e cádmio sobre Ceriodaphnia dubia, por multivias de exposição e recuperação biológica de descendentes

Directory of Open Access Journals (Sweden)

Marcela Merides Carvalho

2017-08-01

Full Text Available RESUMO Os metais frequentemente são avaliados em águas doces como soluções dissolvidas, assumindo que o efeito tóxico é causado unicamente por via aquática (respiração e contato. No entanto, estudos abrangendo concomitantemente a toxicidade na água e no alimento, como acontece no meio, são pouco discutidos na literatura. No presente estudo, a toxicidade de zinco e cádmio foi avaliada expondo-se Ceriodaphnia dubia simultaneamente ao alimento e ao meio aquoso. A espécie de alga verde Raphidocelis subcapitata foi exposta durante 96h a concentrações de Zn (0,18 e 0,27 mg.L-1 e Cd (0,001 e 0,0015 mg.L-1. Os resultados foram analisados estatisticamente por meio da Análise de Variância (Kruskal-Wallis. As algas foram usadas como fonte de alimento para C. dubia, durante exposição crônica (oito dias, nas mesmas concentrações. Posteriormente, os neonatos (geração F1 foram introduzidos em água e alimentação sem contaminantes, para averiguação da capacidade de recuperação biológica. Foram avaliados número de neonatos por indivíduos, morfologia dos neonatos e quantificação dos metais em tecido biológico. Os resultados demonstraram que nas concentrações testadas não houve inibição no crescimento de R. subcapitata, enquanto para C. dubia evidenciou-se toxicidade crônica pela redução na taxa reprodutiva nas duas gerações, para ambos metais. Concluiu-se que, mesmo em concentrações relativamente baixas, os metais zinco e cádmio podem alterar o padrão reprodutivo dos invertebrados de água doce, comprometendo o ecossistema aquático e sua capacidade de recuperação. Considerando os efeitos tóxicos desses metais e sua interferência no sistema biológico, novos ensaios ecotoxicológicos devem ser realizados para melhor compreensão do comportamento dessas substâncias nos organismos.

Armazenamento de sementes de camu-camu (Myrciaria dubia com diferentes graus de umidade e temperaturas

Directory of Open Access Journals (Sweden)

Ferreira Sidney Alberto do Nascimento

2003-01-01

Full Text Available O objetivo do presente trabalho foi avaliar a qualidade fisiológica das sementes de camu-camu (Myrciaria dubia (H.B.K. McVaugh, com diferentes graus de umidade (46 e 40%, submetidas a diferentes condições (temperatura ambiente - média de 26,5°C, câmara a 20°C e câmara a 10°C e períodos de armazenamento (30, 60, 90, 120 e 150 dias. Após cada período, foram retiradas amostras dos tratamentos para as avaliações do grau de umidade e da qualidade fisiológica das sementes. A redução do grau de umidade afetou negativamente a viabilidade e o vigor das sementes, e a temperatura de 10°C não foi favorável ao armazenamento das sementes. Assim, as sementes de camu-camu devem ser armazenadas com grau de umidade elevado (próximo a 46% e, preferencialmente, sob temperatura de 20°C, para manter a viabilidade e o vigor por maior período de tempo.

Nutritional composition and vitamin C stability in stored camu-camu (Myrciaria dubia) pulp.

Science.gov (United States)

Justi, K C; Visentainer, J V; Evelázio de Souza, N; Matsushita, M

2000-12-01

Camu-camu (Myrciaria dubia), a native fruit of the Amazon region, is one of the richest sources of vitamin C (2.4 to 3.0 g/100 g in the pulp) found in Brazil. The purpose of this work was the physical-chemical characterization of some nutrients and the valuation of vitamin C stability in stored camu-camu pulp, produced by the Agronomic Institute of Paraná (IAPAR), Paraná State, Brazil. The vitamin C determination was made by titration with potassium iodate. The fruit produced in Paraná State, presented a lower content of vitamin C than the one native of the amazon region, possibly due to the different development conditions of the plant, and consequently of the fruit, as well as the climatic variation, the humidity and the characteristics of the soil. Regarding the vitamin C stability in stored (-18 degrees C) camu-camu pulp, a considerable decrease in its concentration until the 28th day was observed lost 23% (from 1.57 to 1.21 g/100 g), staying approximately the same until the end of the experiment. After 335 days of storage, the content found was of approximately 1.16 g/100 g of pulp, the ascorbic acid losses amounted to 26%. This content was still higher than the one found for most fruits that are good sources of this vitamin.

Seeds and plantlets of Myrciaria dubia “camu-camu”: biometry, germination, and initial growth

Directory of Open Access Journals (Sweden)

Anderson E. Medina Bardales

2014-06-01

Full Text Available The objectives were to determine seed biometrics, recording cellular respiration in the imbibition process, evaluate the effect of inducing chemicals in germination and early growth analysis. Cellular respiration during imbibition was measured by reduction of methylene blue and Janus Green B. The inductor effect of five chemical compounds in germination was evaluated. Initial growth was recorded by stereoscopic. Results showed that the seeds have an average weight of 520 ± 172 mg, length of 13.50 ± 1.76 mm, width of 10.61 ± 1.14 mm, and thickness of 4.68 ± 0 62 mm. These variables showed significant correlations (r of 0.494 to 0.897 and p 90% and 65% respectively. Seedling initial growth consisted of six stages and occurred over a period of 22 days after radicle emergence. In conclusion, M. dubia seeds show wide variation in their biometrics (weight, length, width and thickness, but show significant correlations with each other. Likewise, seeds are metabolically active in imbibition process, and germination is induced by sodium nitrite and potassium cyanide. Furthermore, seedling initial growth to occur over a period of 22 days after emergence of the radicle and consists of six distinct stages.

Converging on the Clinch River: the politics of the US breeder reactor program

International Nuclear Information System (INIS)

Tench, H.G.

1981-01-01

For decades the development of nuclear technologies, including the fast breeder, was controlled by the Atomic Energy Commission, the Joint Committee on Atomic Energy, and the nuclear industry, the three pillars of the nuclear technoscience network, the analysis of the formation, culture, growth, and disruption of the network sets the stage for the study of the fast breeder, a program conceived by the scientific estate and carried along and nurtured by the administrative and political estates of the nuclear technoscience network, the early chapters delineate the dimensions of the network. The relationships and influence of its actors, and its unique and secretive culture. The cooperative-client politics of the breeder's deployment ended when the program, for years protected within the interstices of the network, emerged from this protected environment into the larger political system, where it became the subject of Washington's entrepreneurial politics. Under these changed circumstances progress on the LMFBR came to a virtual standstill. Nevertheless, the LMFBR survived the turbulent seventies, when energy and the environment dominate the political agenda, because remnants of the nuclear technoscience network in the executive branch, in Congress, and in the private sector retained sufficient power to prevent the breeder's death. This case study of the politics of the breeder's deployment addresses the dilemma of the experts, their role in the decision-making process as well as the problem of uncertainty within the framework of network theory. This perspective allows for the examination of the multifaceted controversy over the deployment of the fast breeder in the United States, a national debate that eventually converged on the Clinch River

Influence of glyphosate and its formulation (Roundup[reg]) on the toxicity and bioavailability of metals to Ceriodaphnia dubia

Energy Technology Data Exchange (ETDEWEB)

Tsui, Martin T.K. [Department of Biology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong (China); Department of Biology, Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong (China); Wang Wenxiong [Department of Biology, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong (China); Chu, L.M. [Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong (China)]. E-mail: leemanchu@cuhk.edu.hk

2005-11-15

This study examined the toxicological interaction between glyphosate (or its formulation, Roundup[reg]) and several heavy metals to a freshwater cladoceran, Ceriodaphnia dubia. We demonstrated that all binary combinations of Roundup[reg] and metals (Cd, Cu, Cr, Ni, Pb, Se and Zn) exhibited 'less than additive' mixture toxicity, with 48-h LC50 toxic unit>1. Addition of glyphosate alone could significantly reduce the acute toxicity of Ag, Cd, Cr, Cu, Ni, Pb and Zn (but not Hg and Se). The ratio between glyphosate and metal ions was important in determining the mitigation of metal toxicity by glyphosate. A bioaccumulation study showed that in the presence of glyphosate the uptake of some metals (e.g. Ag) was halted but that of others (e.g. Hg) was increased significantly. Therefore, our study strongly suggests that glyphosate and its commercial formulations can control the toxicity as well as the bioavailability of heavy metals in aquatic ecosystems where both groups of chemicals can co-occur. - Glyphosate can control the toxicity and bioavailability of many heavy metals in the aquatic environment.

Influence of glyphosate and its formulation (Roundup[reg]) on the toxicity and bioavailability of metals to Ceriodaphnia dubia

International Nuclear Information System (INIS)

Tsui, Martin T.K.; Wang Wenxiong; Chu, L.M.

2005-01-01

This study examined the toxicological interaction between glyphosate (or its formulation, Roundup[reg]) and several heavy metals to a freshwater cladoceran, Ceriodaphnia dubia. We demonstrated that all binary combinations of Roundup[reg] and metals (Cd, Cu, Cr, Ni, Pb, Se and Zn) exhibited 'less than additive' mixture toxicity, with 48-h LC50 toxic unit>1. Addition of glyphosate alone could significantly reduce the acute toxicity of Ag, Cd, Cr, Cu, Ni, Pb and Zn (but not Hg and Se). The ratio between glyphosate and metal ions was important in determining the mitigation of metal toxicity by glyphosate. A bioaccumulation study showed that in the presence of glyphosate the uptake of some metals (e.g. Ag) was halted but that of others (e.g. Hg) was increased significantly. Therefore, our study strongly suggests that glyphosate and its commercial formulations can control the toxicity as well as the bioavailability of heavy metals in aquatic ecosystems where both groups of chemicals can co-occur. - Glyphosate can control the toxicity and bioavailability of many heavy metals in the aquatic environment

Site suitability report in the matter of Clinch River Breeder Reactor Plant. Docket No. 50-537. Revision to March 4, 1977 report

International Nuclear Information System (INIS)

1982-06-01

In March 1977, the Office of Nuclear Reactor Regulation issued its Site Suitability Report (SSR) for the proposed Clinch River Breeder Plant (CRBRP). That SSR documents the result of the staff's evaluation of the suitability of the proposed CRBRP site for a facility of the general size and type as the CRBRP from the standpoint of radiological health and safety considerations. The staff concluded in that SSR that the proposed CRBRP site is suitable for such a facility. Since the SSR was issued, several modifications have been made to the CRBRP design, additional data related to the site and its environs have been collected, and the Fast Flux Test Facility, a technological precursor to the CRBRP, has been completed and has commenced operation. In addition, new emergency planning requirements have been promulgated by the staff. This report is an update of the March 1977 SSR that reflects these matters and discusses them in terms of the previous staff conclusion regarding the suitability of the proposed CRBRP site

Reproductive success and mortality rates of Ceriodaphnia dubia maintained in water from Upper Three Runs, Pen Branch, and Fourmile Branch

International Nuclear Information System (INIS)

Specht, W.L.

1994-12-01

It is anticipated that the new SRS NPDES permit will require toxicity testing of at numerous outfalls and receiving streams, using the standard test species, Ceriodaphnia dubia. Because SRS surface waters differ markedly from the standard culture water that is used for Ceriodaphnia, studies were undertaken to determine if unimpacted SRS surface waters will support this species. Three SRS surface waters were evaluated; Upper Three Runs at Road 8-1, Pen Branch at Road B, and Fourmile Branch at Road F. Toxicity tests were performed monthly on each water source for eleven months. All three water sources exhibited varying degrees of toxicity to Ceriodaphnia, with Pen Branch being the least toxic and Fourmile Branch being the most toxic. These results indicate that if in-stream toxicity testing is required, it may not be possible to separate the naturally occurring toxic effects of the receiving water from possible toxic effects of SRS effluents

Tropical fruit camu-camu (Myrciaria dubia) has anti-oxidative and anti-inflammatory properties.

Science.gov (United States)

Inoue, Teruo; Komoda, Hiroshi; Uchida, Toshihiko; Node, Koichi

2008-10-01

Oxidative stress as well as inflammation plays a pivotal role in the pathogenesis of atherosclerosis. Although, various anti-oxidative dietary supplements have been evaluated for their ability to prevent atherosclerosis, no effective ones have been determined at present. "Camu-camu" (Myrciaria dubia) is an Amazonian fruit that offers high vitamin C content. However, its anti-oxidative property has not been evaluated in vivo in humans. To assess the anti-oxidative and anti-inflammatory properties of camu-camu in humans, 20 male smoking volunteers, considered to have an accelerated oxidative stress state, were recruited and randomly assigned to take daily 70 ml of 100% camu-camu juice, corresponding to 1050 mg of vitamin C (camu-camu group; n=10) or 1050 mg of vitamin C tablets (vitamin C group; n=10) for 7 days. After 7 days, oxidative stress markers such as the levels of urinary 8-hydroxy-deoxyguanosine (P<0.05) and total reactive oxygen species (P<0.01) and inflammatory markers such as serum levels of high sensitivity C reactive protein (P<0.05), interleukin (IL)-6 (P<0.05), and IL-8 (P<0.01) decreased significantly in the camu-camu group, while there was no change in the vitamin C group. Our results suggest that camu-camu juice may have powerful anti-oxidative and anti-inflammatory properties, compared to vitamin C tablets containing equivalent vitamin C content. These effects may be due to the existence of unknown anti-oxidant substances besides vitamin C or unknown substances modulating in vivo vitamin C kinetics in camu-camu.

The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: the effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats.

Science.gov (United States)

Gonzales, Gustavo F; Vasquez, Vanessa Bertha; Gasco, Manuel

2013-10-01

Transillumination technique for assessment of stages of spermatogenic cycle is a useful tool for toxicological studies. This study was designed to determine the effect of two medicinal plants on spermatogenesis in male rats using the transillumination technique. For this, the effect of the combination of a fruit with highest content of ascorbic acid (Myrciaria dubia, camu camu) and extract of black maca (Lepidium meyenii) on seminiferous tubule stages scored by transillumination on intact tubules in adult male rats was assessed. Animals were treated during seven days with vehicle, black maca, camu camu or a mixture of black maca + camu camu and assessed for daily sperm production (DSP), stages of spermatogenic cycle as well as antioxidant activity and levels of flavonoids and polyphenols. Black maca increased stages of spermiation (VII-VIII) and mitosis of germ cells (IX-XI), whereas camu camu increased stages of mitosis (IX-XI) and meiosis (XII). Mixture of maca + camu camu increased stages of spermiation, mitosis and meiosis. All treatments increased DSP (pmaca. In conclusion, M. dubia (camu camu) has potential effects improving spermatogenesis and co-administered with maca increase stages of mitosis, meiosis and spermiation of the spermatogenic cycle as assessed by the transillumination technique. This technique is becoming increasingly a useful tool for assessment spermatogenesis.

Influence of exposure time on the distribution of cadmium within the cladoceran Ceriodaphnia dubia

International Nuclear Information System (INIS)

Munger, C.; Hare, L.; Craig, A.; Charest, P.-M.

1998-01-01

The internal distribution of a trace metal can be useful in determining in which body organ toxic effects are most likely to occur and the animal function most likely to be affected. In addition, because a metal's distribution within an organism is likely to influence the rate and efficiency with which it is transferred to a higher trophic level, laboratory measurements of trophic transfer could be influenced by exposure duration. We compared the internal distribution of cadmium (Cd) in a cladoceran crustacean (Ceriodaphnia dubia) destined as food for a predatory insect (Chaoborus) after either a 1 day or a lifetime of exposure of prey to the trace metal. Prey were exposed to 112+109 Cd in both water (10 nM) and their food (algae), as might occur in nature. The internal distribution of 109 Cd in the cladoceran was determined by whole-animal autoradiography. Both the amount and the tissue distribution of Cd in prey were the same after the short and the long term exposures, suggesting that metal accumulation parameters measured after short-term metal exposures can be valid for this animal. Cadmium was mainly accumulated in diverticula of the anterior midgut, a region reported to be responsible for nutrient absorption. We hypothesize that Cd is accumulated in the diverticula because of their purported role as sites of calcium uptake. (Copyright (c) 1998 Elsevier Science B.V., Amsterdam. All rights reserved.)

Contaminant characterization of sediment and pore-water in the Clinch River and Poplar Creek

International Nuclear Information System (INIS)

Levine, D.A.; Harris, R.A.; Campbell, K.R.; Hargrove, W.W.; Rash, C.D.

1995-01-01

Sediment and pore-water samples were collected from 80 locations in the Clinch River and Poplar Creek system to characterize concentrations and spatial distribution of contaminants for use in ecological risk assessment. Sediment cores were collected at each site and the top 15 cm was analyzed to represent the biologically active zone. Sediment for pore-water extraction was collected in large volumes using a Ponar grab sampler. Pore-water was extracted from this sediment using centrifugation, All samples were analyzed for metals (including methyl mercury), organics, and radiological constituents. Additionally, sediment was analyzed for physical properties: particle size distribution, density, and porosity. Sediment and pore-water were also analyzed for total organic carbon and nitrogen and ammonia levels. Sediment and pore-water were also analyzed for total organic carbon and nitrogen and ammonia levels. Sediment and pre-water results indicate that there are several areas where concentrations of a variety of contaminants are high enough to causes ecological effects. These locations in the river are immediately downstream from know sources of Contamination from on-site DOE facilities. East Fork Poplar Creek is a source of several metals, including mercury, cadmium, chromium, and copper. Mitchell Branch is a source of number of metals, uranium isotopes, technetium-99, and several PAHs. There are two clear sources of arsenic and selenium to the system, one in Poplar Creek and one in Melton Hill Reservoir, both related to past disposal of coal-ash. High concentrations in sediments did not always coincide with high concentrations in pore-water for the same sites and contaminants. This appears to be related to particle size of the sediment and total organic carbon

Post-harvest conservation of camu–camu fruits (Myrciaria dubia (Kunth Mc Vaugh using different temperatures and packages

Directory of Open Access Journals (Sweden)

Maria Luiza GRIGIO

2015-12-01

Full Text Available Abstract The camu-camu tree (Myrciaria dubia (Kunth Mc Vaugh is fruit-bearing tree belonging to the family Myrtaceae. This work was conducted with the purpose of evaluating the type of storage temperature and package which allow better conservation of the quality attributes of camu-camu. The experimental design utilized was the completely randomized with three replications in a factorial arrangement (3x3x8, constituted of three different storage temperatures (laboratory ambiente or 25 ± 2 °C, 15 °C and 20 °C, three types of packages (no package, PET and PVC and fourteen days’ storage, the fruits being analyzed every two days. The fruits were evaluated as to fresh mass loss, pH, soluble solids contents, titrable acidity, ascorbic acid, carotenoids, anthocyanins, chlorophylls A and B and maturation index (SS/AT. According to the results obtained, the quality attributes and ascorbic acid content were conserved for longer time in the fruits stored on PVC-film covered expanded polystyrene trays at 15 °C. It follows that the best temperature for the storage of camu-camu is 15 °C and the package that best keeps its quality attributes is the PVC-film covered expanded polystyrene tray.

Oak Ridge National Laboratory Biological Monitoring and Abatement Program for White Oak Creek Watershed and the Clinch River

International Nuclear Information System (INIS)

Loar, J.M.; Adams, S.M.; Allison, L.J.; Blaylock, B.G.; Boston, H.L.; Huston, M.A.; Kimmel, B.L.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.; Walton, B.T.; Kitchings, J.T.; Olsen, C.R.

1991-09-01

On April 1, 1986, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge National Laboratory (ORNL) (EPA 1986). As specified in Part 3: Special Conditions (Item H) of the permit, a plan for biological monitoring of the Clinch River, White Oak Creek (WOC), Northwest Tributary (NWT) of WOC, Melton Branch (MB), Fifth Creek, and First Creek shall be submitted for approval to the US Environmental Protection Agency (EPA) and the Tennessee Department of Health and Environment (TDHE) within 90 days of the effective date of the permit. The plan, which is referred to in Part 3 (H) of the permit as the Biological Monitoring Plan and Abatement Program (BMPAP), describes characterization monitoring studies to be conducted for the duration of the permit (5 years). In order to be consistent with the terminology used for the Biological Monitoring and Abatement Programs for the Oak Ridge Y-12 Plan and the Oak Ridge K-25 Plant, BMPAP will subsequently be referred to as the Biological Monitoring and Abatement Program (BMAP). The proposed BMAP outlined in this document is based on preliminary discussions held on December 9, 1985, between staff of Martin Marietta Energy Systems, Inc. (ORNL and Central Management), the US Department of Energy (DOE), EPA, and TDHE. 232 refs., 11 figs., 7 tabs

Oak Ridge National Laboratory Biological Monitoring and Abatement Program for White Oak Creek Watershed and the Clinch River

Energy Technology Data Exchange (ETDEWEB)

Loar, J.M.; Adams, S.M.; Allison, L.J.; Blaylock, B.G.; Boston, H.L.; Huston, M.A.; Kimmel, B.L.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.; Walton, B.T.; Kitchings, J.T.; Olsen, C.R.

1991-09-01

On April 1, 1986, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge National Laboratory (ORNL) (EPA 1986). As specified in Part 3: Special Conditions (Item H) of the permit, a plan for biological monitoring of the Clinch River, White Oak Creek (WOC), Northwest Tributary (NWT) of WOC, Melton Branch (MB), Fifth Creek, and First Creek shall be submitted for approval to the US Environmental Protection Agency (EPA) and the Tennessee Department of Health and Environment (TDHE) within 90 days of the effective date of the permit. The plan, which is referred to in Part 3 (H) of the permit as the Biological Monitoring Plan and Abatement Program (BMPAP), describes characterization monitoring studies to be conducted for the duration of the permit (5 years). In order to be consistent with the terminology used for the Biological Monitoring and Abatement Programs for the Oak Ridge Y-12 Plan and the Oak Ridge K-25 Plant, BMPAP will subsequently be referred to as the Biological Monitoring and Abatement Program (BMAP). The proposed BMAP outlined in this document is based on preliminary discussions held on December 9, 1985, between staff of Martin Marietta Energy Systems, Inc. (ORNL and Central Management), the US Department of Energy (DOE), EPA, and TDHE. 232 refs., 11 figs., 7 tabs.

Polpa de camu-camu (Myrciaria dubia submetida à radiação gama

Directory of Open Access Journals (Sweden)

Jacqueline de Oliveira

2013-01-01

Full Text Available O fruto amazônico camu-camu, Myrciaria dubia, possui elevado teor de vitamina C, antocianinas, carotenóides e compostos fenólicos, fatores que fazem com este fruto venham se posicionando na preferência dos consumidores de frutas exóticas. A radiação ionizante tem sido utilizada para aumentar a vida útil dos produtos, além de preservar qualidades intrínsecas e nutricionais. Desta forma, o objetivo deste trabalho foi estudar a radiação gama como método alternativo na conservação da polpa de camu-camu. As amostras de polpa do fruto foram submetidas às doses 0 (controle, 2, 4, e 6 kGy. Em seguida, foram armazenadas a 6 ºC e à temperatura ambiente (26 ºC, e avaliadas quanto a cor, pH, acidez titulável, teores de sólidos solúveis, vitamina C, compostos fenólicos e antocianinas nos períodos 1 e 15 dias de armazenamento. Os atributos pH, teor de sólidos solúveis, compostos fenólicos e acidez total, não foram afetados pela irradiação e pela temperatura de armazenamento nos dois períodos analisados. A vitamina C também não se alterou com a irradiação, porém o armazenamento refrigerado manteve os teores mais constantes. A cor foi o atributo mais afetado pela irradiação e pelo armazenamento à temperatura ambiente. Portanto, a radiação gama não se mostrou eficaz no armazenamento da polpa desta fruta em nenhuma das temperaturas propostas.

Aproveitamento do camu-camu (Myrciaria dubia para produção de bebida alcoólica fermentada The use of camu-camu (Myrciaria dubia for the production of a fermented alcoholic beverage

Directory of Open Access Journals (Sweden)

Roberto Nobuyuki Maeda

2003-01-01

Full Text Available O elevado teor de ácido ascórbico no camu-camu (Myrciaria dubia McVaugh, Myrtaceae desperta o interesse de extrativistas, agricultores e consumidores, e leva à necessidade de desenvolvimento de tecnologias adequadas para produção em terra firme e aproveitamento industrial do fruto. Este trabalho teve por objetivo verificar a adequação do camu-camu para a produção de bebida alcoólica fermentada, assim como o efeito do branqueamento do fruto e da incorporação da casca à polpa nas características nutricionais e sensoriais da bebida. Os frutos foram separados em quatro lotes, sendo dois branqueados (90 ºC por 7 min. Após a despolpa, as cascas de um lote de cada tratamento (com e sem branqueamento foram incorporadas às respectivas polpas e avaliadas quanto à composição química (umidade, pH, acidez, sólidos solúveis, açúcares, ácido ascórbico, compostos fenólicos, antocianinas e flavonóides. Após a correção do mosto com açúcar, pasteurização, fermentação (25 dias, trasfega, pasteurização (70 ºC por 15 min, filtragem e clarificação, as bebidas foram avaliadas quanto a composição química, edulcoradas e submetidas à análise sensorial. O branqueamento reduziu a concentração de ácido ascórbico das polpas (33 % e a agregação da casca aumentou os teores de matéria seca (39 % polpa, ácido ascórbico (33 % na polpa, 23 % no mosto e 50 % na bebida e fenólicos (50 % bebida. O perfil sensorial e a aceitabilidade sugerem que o camu-camu é adequado para a produção de bebida alcoólica fermentada e que a agregação da casca à polpa contribuiu positivamente para a aceitabilidade (6,7 com casca e 6,2 sem casca, na escala de 9 pontos. As bebidas apresentaram flavor característico do fruto, limpidez, coloração vermelho-laranjada e sabor agradável.The high levels of ascorbic acid in camu-camu (Myrciaria dubia McVaugh, Myrtaceae have stimulated interest of extractivists, farmers and consumers. This has led

The orange spotted cockroach (Blaptica dubia, Serville 1839) is a permissive experimental host for Francisella tularensis

Science.gov (United States)

Eklund, Bridget E.; Mahdi, Osama; Huntley, Jason F.; Collins, Elliot; Martin, Caleb; Horzempa, Joseph; Fisher, Nathan A.

2018-01-01

Francisella tularensis is a zoonotic bacterial pathogen that causes severe disease in a wide range of host animals, including humans. Well-developed murine models of F. tularensis pathogenesis are available, but they do not meet the needs of all investigators. However, researchers are increasingly turning to insect host systems as a cost-effective alternative that allows greater increased experimental throughput without the regulatory requirements associated with the use of mammals in biomedical research. Unfortunately, the utility of previously-described insect hosts is limited because of temperature restriction, short lifespans, and concerns about the immunological status of insects mass-produced for other purposes. Here, we present a novel host species, the orange spotted (OS) cockroach (Blaptica dubia), that overcomes these limitations and is readily infected by F. tularensis. Intrahemocoel inoculation was accomplished using standard laboratory equipment and lethality was directly proportional to the number of bacteria injected. Progression of infection differed in insects housed at low and high temperatures and F. tularensis mutants lacking key virulence components were attenuated in OS cockroaches. Finally, antibiotics were delivered to infected OS cockroaches by systemic injection and controlled feeding; in the latter case, protection correlated with oral bioavailability in mammals. Collectively, these results demonstrate that this new host system provides investigators with a new tool capable of interrogating F. tularensis virulence and immune evasion in situations where mammalian models are not available or appropriate, such as undirected screens of large mutant libraries. PMID:29578544

Antocianinas, ácido ascórbico, polifenoles totales y actividad antioxidante, en la cáscara de camu-camu (Myrciaria dubia (H.B.K McVaugh Antocianinas, ácido ascórbico, polifenóis totais e atividade antioxidante na casca do camu-camu (Myrciaria dubia (H.B.K McVaugh

Directory of Open Access Journals (Sweden)

Juan Edson Villanueva-Tiburcio

2010-05-01

Full Text Available Este trabajo fue realizado en la UNAS, Tingo María, Perú. Los objetivos fueron evaluar el contenido de antocianinas, ácido ascórbico, y polifenoles totales, en la cáscara fresca y seca de camu-camu (Myrciaria dubia (H.B.K McVaugh en diferentes estados de madurez; evaluar la actividad antioxidante en la cáscara seca, usando diferentes tipos de radicales (DPPH, ABTS+ y Peroxilo y correlacionar el valor de ácido ascórbico y polifenoles totales con la actividad antioxidante. La extracción fue realizada en medio acuoso, y los resultados de las evaluaciones de cada experimento fueron analizados por un diseño completamente al azar (DCA, según la prueba de t-student (p A pesquisa foi realizada na UNAS em Tingo Maria, Peru, teve como objetivos: avaliar o teor de antocianinas, ácido ascórbico e polifenóis totais, na casca fresca e na casca seca do camu-camu (Myrciaria dubia (HBK McVaugh em diferentes tempos de maturação; avaliar a atividade antioxidante na casca seca utilizando diferentes tipos de radicais (DPPH, ABTS+ e Peroxilo e correlacionar o teor de ácido ascórbico e polifenóis totais com a atividade antioxidante. A extraç��o foi realizada em meio aquoso, os resultados das avaliações de cada experiência são analisados no delineamento inteiramente casualizado (DIC, pelo teste de t-student (p < 0,05. O extrato da casca da amostra madura fresca apresentou as concentrações mais elevadas de ácido ascórbico e de antocianinas em relação a meio madura e verde, com 21,95 mg.g-1 de casca e 46,42 mg.L-1 de cianidin-3-glucosídeo, respectivamente, enquanto que o extrato da casca seca meio madura apresentou o maior teor de ácido ascórbico em relação a madura e verde (53,49 mg.g-1 e de polifenóis totais: 7,70 mg Ác. Gálico/g. A maior atividade antioxidante foi encontrada no extrato da casca seca da amostra meio madura com IC50 = 46,20; 20,25 e 8,30 μg.mL-1, em comparação com DPPH radical, ABTS+ e Perox

Acute and chronic toxicity of uranium compounds to Ceriodaphnia-Daphnia dubia

International Nuclear Information System (INIS)

Pickett, J.B.; Specht, W.L.; Keyes, J.L.

1993-01-01

A study to determine the acute and chronic toxicity of uranyl nitrate, hydrogen uranyl phosphate, and uranium dioxide to the organism Ceriodaphnia dubia was conducted. The toxicity tests were conducted by two independent environmental consulting laboratories. Part of the emphasis for this determination was based on concerns expressed by SCDHEC, which was concerned that a safety factor of 100 must be applied to the previous 1986 acute toxicity result of 0.22 mg/L for Daphnia pulex, This would have resulted in the LETF release limits being based on an instream concentration of 0.0022 mg/L uranium. The NPDES Permit renewal application to SCDHEC utilized the results of this study and recommended that the LETF release limit for uranium be based an instream concentration of 0.004 mg/L uranium. This is based on the fact that the uranium releases from the M-Area LETF will be in the hydrogen uranyl phosphate form, or a uranyl phosphate complex at the pH (6--10) of the Liquid Effluent Treatment Facility effluent stream, and at the pH of the receiving stream (5.5 to 7.0). Based on the chronic toxicity of hydrogen uranyl phosphate, a lower uranium concentration limit for the Liquid Effluent Treatment Facility outfall vs. the existing NPDES permit was recommended: The current NPDES permit ''Guideline'' for uranium at outfall M-004 is 0.500 mg/L average and 1.0 mg/L maximum, at a design flowrate of 60 gpm. It was recommended that the uranium concentration at the M-004 outfall be reduced to 0.28 mg/L average, and 0.56 mg/L, maximum, and to reduce the design flowrate to 30 gpm. The 0.28 mg/L concentration will provide an instream concentration of 0.004 mg/L uranium. The 0.28 mg/L concentration at M-004 is based on the combined flows from A-014, A-015, and A-011 outfalls (since 1985) of 1840 gpm (2.65 MGD) and was the flow rate which was utilized in the 1988 NPDES permit renewal application

Remedial investigation/feasibility study of the Clinch River/Poplar Creek operable unit. Volume 1, main text

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-03-01

This document is the combined Remedial Investigation/Feasibility Study (RI/FS) Report for the Clinch River/Poplar Creek Operable Unit (CR/PC OU), an off-site OU associated with environmental restoration activities at the U.S. Department of Energy (DOE) Oak Ridge Reservation (ORR). As a result of past, present, and potential future releases of hazardous substances into the environment, the ORR was placed on the National Priorities List in December 1989 (54 FR 48184). Sites on this list must be investigated for possible remedial action, as required by the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA, 42 U.S.C. 9601, et seq.). This report documents the findings of the remedial investigation of this OU and the feasibility of potential remedial action alternatives. These studies are authorized by Sect. 117 of CERCLA and were conducted in accordance with the requirements of the National Contingency Plan (40 CFR Part 300). DOE, the U.S. Environmental Protection Agency (EPA), and the Tennessee Department of Environment and Conservation (TDEC) have entered into a Federal Facility Agreement (FFA), as authorized by Sect. 120 of CERCLA and Sects. 3008(h) and 6001 of the Resource Conservation and Recovery Act (RCRA) (42 U.S.C. 6901, et seq.). The purpose of this agreement is to ensure a coordinated and effective response for all environmental restoration activities occurring at the ORR. In addition to other responsibilities, the FFA parties mutually define the OU boundaries, set remediation priorities, establish remedial investigation priorities and strategies, and identify and select remedial actions. A copy of this FFA is available from the DOE Information Resource Center in Oak Ridge, Tennessee.

Remedial investigation/feasibility study of the Clinch River/Poplar Creek operable unit. Volume 1, main text

International Nuclear Information System (INIS)

1996-03-01

This document is the combined Remedial Investigation/Feasibility Study (RI/FS) Report for the Clinch River/Poplar Creek Operable Unit (CR/PC OU), an off-site OU associated with environmental restoration activities at the U.S. Department of Energy (DOE) Oak Ridge Reservation (ORR). As a result of past, present, and potential future releases of hazardous substances into the environment, the ORR was placed on the National Priorities List in December 1989 (54 FR 48184). Sites on this list must be investigated for possible remedial action, as required by the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA, 42 U.S.C. 9601, et seq.). This report documents the findings of the remedial investigation of this OU and the feasibility of potential remedial action alternatives. These studies are authorized by Sect. 117 of CERCLA and were conducted in accordance with the requirements of the National Contingency Plan (40 CFR Part 300). DOE, the U.S. Environmental Protection Agency (EPA), and the Tennessee Department of Environment and Conservation (TDEC) have entered into a Federal Facility Agreement (FFA), as authorized by Sect. 120 of CERCLA and Sects. 3008(h) and 6001 of the Resource Conservation and Recovery Act (RCRA) (42 U.S.C. 6901, et seq.). The purpose of this agreement is to ensure a coordinated and effective response for all environmental restoration activities occurring at the ORR. In addition to other responsibilities, the FFA parties mutually define the OU boundaries, set remediation priorities, establish remedial investigation priorities and strategies, and identify and select remedial actions. A copy of this FFA is available from the DOE Information Resource Center in Oak Ridge, Tennessee

Fractionation of fulvic acid by iron and aluminum oxides: influence on copper toxicity to Ceriodaphnia dubia

Science.gov (United States)

Smith, Kathleen S.; Ranville, James F.; Lesher, Emily K.; Diedrich, Daniel J.; McKnight, Diane M.; Sofield, Ruth M.

2014-01-01

This study examines the effect on aquatic copper toxicity of the chemical fractionation of fulvic acid (FA) that results from its association with iron and aluminum oxyhydroxide precipitates. Fractionated and unfractionated FAs obtained from streamwater and suspended sediment were utilized in acute Cu toxicity tests on ,i>Ceriodaphnia dubia. Toxicity test results with equal FA concentrations (6 mg FA/L) show that the fractionated dissolved FA was 3 times less effective at reducing Cu toxicity (EC50 13 ± 0.6 μg Cu/L) than were the unfractionated dissolved FAs (EC50 39 ± 0.4 and 41 ± 1.2 μg Cu/L). The fractionation is a consequence of preferential sorption of molecules having strong metal-binding (more aromatic) moieties to precipitating Fe- and Al-rich oxyhydroxides, causing the remaining dissolved FA to be depleted in these functional groups. As a result, there is more bioavailable dissolved Cu in the water and hence greater potential for Cu toxicity to aquatic organisms. In predicting Cu toxicity, biotic ligand models (BLMs) take into account dissolved organic carbon (DOC) concentration; however, unless DOC characteristics are accounted for, model predictions can underestimate acute Cu toxicity for water containing fractionated dissolved FA. This may have implications for water-quality criteria in systems containing Fe- and Al-rich sediment, and in mined and mineralized areas in particular. Optical measurements, such as specific ultraviolet absorbance at 254 nm (SUVA254), show promise for use as spectral indicators of DOC chemical fractionation and inferred increased Cu toxicity.

Leachate From Expanded Polystyrene Cups Is Toxic to Aquatic Invertebrates (Ceriodaphnia dubia

Directory of Open Access Journals (Sweden)

Clara Thaysen

2018-02-01

Full Text Available Expanded polystyrene (EPS products and their associated chemicals (e.g., styrenes are widespread in the marine environment. As a consequence, bans on their use for single-use packaging materials are being proposed in several municipalities. To better understand how science can inform decision-making, we looked at the available scientific literature about contamination and effects and conducted experiments to measure chemical leachate from polystyrene products and toxicity from the leachate. We conducted leaching experiments with common food matrices (water, soup broth, gravy, black coffee and coffee with cream and sugar at relevant temperatures (70 and 95°C that are consumed in or with several polystyrene products (coffee cup lids, polystyrene stir sticks, polystyrene spoons, EPS cups, EPS bowls, and EPS takeout containers. We analyzed each sample for styrene, ethylbenzene, toluene, benzene, meta- and para- xylene, isopropylbenzene, and isopropyltoluene—chemicals associated with polystyrene products. To determine whether the leachates are toxic, we conducted chronic toxicity tests, measuring survival and reproductive output in Ceriodaphnia dubia. Toxicity tests included nine treatments: seven concentrations of ethylbenzene, EPS cup leachate and a negative control. Overall, we found that temperature has a significant effect on leaching. We only detected leachates in trials conducted at higher temperature −95°C. Ethylbenzene was the only target analyte with final concentrations above the method limit of detection, and was present in the greatest concentrations in EPS and with soup broth. Measurable concentrations of ethylbenzene in the leachate ranged from 1.3 to 3.4 μg/L. In toxicity tests, the calculated LC50 for ethylbenzene was 14 mg/L and the calculated LC20 was 210 μg/L. For the treatment exposed to the EPS cup leachate, mortality was 40%—four times greater than the negative control. Finally, there was no significant difference (p

Esterases no exame da estrutura populacional de Camu-camu (Myrciaria dubia (Kunth McVaugh-Myrtaceae Esterases for examining the population structure of Camu-camu (Myrciaria dubia (Kunth McVaugh-Myrtaceae

Directory of Open Access Journals (Sweden)

Aylton Saturnino Teixeira

2004-01-01

Full Text Available Dois sistemas enzimáticos (esterase e esterase-D, analisados pela técnica de eletroforese em gel de amido, em folhas jovens de plantas cultivadas em terra firme, de sementes provenientes de três amostras de populações naturais de camu-camu, Myrciaria dubia (Kunth McVaugh-Myrtaceae, procedentes de Iquitos, Boa Vista e Uatumã, revelaram a presença de 6 locos: Est-1, Est-2, Est-3, Est-4, Est-D1 e Est-D2. Dois dos seis locos gênicos examinados no presente estudo (Est-3 e Est-D2 mostraram-se polimórficos, sendo desse modo considerados valiosos no estudo de caracterização da estrutura populacional da espécie. Os padrões de polimorfismo revelados nos locos Est-3 e Est-D2 de camu-camu, são típicos de enzimas monoméricas e diméricas, respectivamente. O loco Est-3 apresentou um grande desbalanço genético dentro e entre as amostras populacionais examinadas, devido ao excessivo número observado de plantas heterozigóticas em relação ao número esperado. O loco Est-D2 apresentou um polimorfismo exclusivo para os alelos Est-D2¹,Est-D2² e Est-D2³, e um bom balanço genético na amostra populacional de Uatumã. Em função disso, dentre os demais locos gênicos aqui investigados, o loco Est-D2 parece ser o mais adequado para identificação e delimitação de prováveis estoques de camu-camu. Portanto, recomenda-se que esse loco esteja presente na lista dos marcadores isoenzimáticos a serem usados em futuras prospecções sobre genética populacional dessa espécie na região amazônica. Dados sobre a distribuição das freqüências alélicas, estimativas das distâncias genéticas, e estimativas de variação genética nos 6 locos de esterases examinados, foram eficazes na demonstração de diferenças genéticas entre as amostras populacionais examinadas da espécie. Os maiores valores de heterozigozidade média (0,1353; proporção de locos polimórficos (0,33 e número médio de alelos por loco (1,33 revelados na amostra

Ocorrência de Tuthillia cognata Hodkinson, Brown & Burckhardt, 1986(Hemiptera: Homoptera, Psyllidae em plantios experimentais de camu-camu Myrciaria dubia (H.B.K. Mc Vaugh em Manaus (Amazonas, Brasil Occurrence of Tuthillia cognata Hodkinson, Brown & Burckhardt, 1986 (Hemiptera: Homoptera, Psyllidae in experimental plantations of camu-camu Myrciaria dubia (H.B.K. Mc Vaugh in Manaus (Amazonas, Brazil

Directory of Open Access Journals (Sweden)

Márcio Luís Leitão Barbosa

2004-01-01

Full Text Available O cultivo de camu-camu Myrciaria dubia (H.B.K. Mc Vaugh tem apresentado inúmeros problemas fitossanitários, dentre os quais, Tuthillia cognata Hodkinson et al. (Hemiptera: Homoptera, Psyllidae, que constantemente é citada como praga secundária. Os objetivos deste estudo foram determinar o nível e a intensidade de infestação (% por T. cognata e estudar aspectos do ciclo biológico e do comportamento de T. cognata, em plantios experimentais de camu-camu. Foram selecionados, de forma aleatória, 17 e 14 exemplares nos plantios I e II, respectivamente. Para cada uma das variáveis estudadas, foram calculados a média aritmética, o desviopadrão, a variância e a amplitude de variação. Foi verificado um nível de infestação de 82% (plantio I e 57% (plantio II, uma intensidade de infestação de 94% (plantio I e 75% (plantio II e uma média de seis ninfas/folha em cada plantio, o que indica que T. cognata representa uma das pragas-chave dessa cultura. Foram observados adultos de Chrysoperla sp. (Neuroptera: Chrysopidae e ninfas de Reduviidae (Hemiptera: Heteroptera, que podem atuar como prováveis agentes de controle biológico de T. cognata.The cultivation of camu-camu Myrciaria dubia (H.B.K. Mc Vaugh has presented countless phytosanitary problems, among them, Tuthillia cognata Hodkinson et al. (Hemiptera: Homoptera, Psyllidae, which often is noticed as a secondary pest. This study aimed to determine the level and the intensity of infestation (% for T. cognata, as well as to study the biological cycle and behavioural aspects of T. cognata, in experimental plantation of camu-camu. 17 and 14 specimens from plantation I and II, respectively, were randomly selected. The arithmetic average, the standard deviation, the variance and the total amplitude were calculated for each studied variable. A level of infestation of 82% (plantation I and 57% (plantation II, an intensity of infestation of 94% (plantation I and 75% (plantation II and an

Effects of diet supplementation with Camu-camu (Myrciaria dubia HBK McVaugh fruit in a rat model of diet-induced obesity

Directory of Open Access Journals (Sweden)

Ozanildo V Nascimento

2013-03-01

Full Text Available Amazonian Camu-camu fruit (Myrciaria dubia HBK Mc Vaugh has attracted interest from food and cosmetics industries because of its rich content of vitamin C, flavonoids and anthocyanins. The goal of this study was investigates the antiobesity action of the ingestion of the Camu-camu pulp in a rat model of diet-induced obesity. Wistar rats with obesity induced by subcutaneous injection of monosodium glutamate receiving diet ad libitum. The rats were divided in two groups: an experimental group that ingested 25 mL/day of Camu-camu pulp (CCG and a non treated group (CG. After 12 weeks, the animals were sacrificed. Blood, liver, heart, white adipose tissues were collected and weighted, biochemical and inflammatory profiles were determinate as well. Animals that received the pulp of Camu-camu reduced their weights of the fat in white adipose tissues, glucose, total cholesterol, triglycerides, LDL-c and insulin blood levels. There was an increase in HDL-c levels. No change was observed in inflammatory markers and liver enzymes. Camu-camu pulp was able to improve the biochemical profile of obesity in rats suggesting that this Amazonian fruit can be further used such a functional food ingredient in control of chronic diseases linked to obesity.

Effects of diet supplementation with Camu-camu (Myrciaria dubia HBK McVaugh) fruit in a rat model of diet-induced obesity.

Science.gov (United States)

Nascimento, Ozanildo V; Boleti, Ana P A; Yuyama, Lucia K O; Lima, Emerson S

2013-03-01

Amazonian Camu-camu fruit (Myrciaria dubia HBK Mc Vaugh) has attracted interest from food and cosmetics industries because of its rich content of vitamin C, flavonoids and anthocyanins. The goal of this study was investigates the antiobesity action of the ingestion of the Camu-camu pulp in a rat model of diet-induced obesity. Wistar rats with obesity induced by subcutaneous injection of monosodium glutamate receiving diet ad libitum. The rats were divided in two groups: an experimental group that ingested 25 mL/day of Camu-camu pulp (CCG) and a non treated group (CG). After 12 weeks, the animals were sacrificed. Blood, liver, heart, white adipose tissues were collected and weighted, biochemical and inflammatory profiles were determinate as well. Animals that received the pulp of Camu-camu reduced their weights of the fat in white adipose tissues, glucose, total cholesterol, triglycerides, LDL-c and insulin blood levels. There was an increase in HDL-c levels. No change was observed in inflammatory markers and liver enzymes. Camu-camu pulp was able to improve the biochemical profile of obesity in rats suggesting that this Amazonian fruit can be further used such a functional food ingredient in control of chronic diseases linked to obesity.

Effects of Spray-Drying Parameters on In Vitro Functional Properties of Camu-Camu (Myrciaria dubia Mc. Vaugh): A Typical Amazonian Fruit.

Science.gov (United States)

Fujita, Alice; Souza, Volnei Brito; Daza, Luis Daniel; Fávaro-Trindade, Carmen Silvia; Granato, Daniel; Genovese, Maria Inés

2017-05-01

Camu-camu (Myrciaria dubia) fruit is a rich source of bioactive compounds but its shelf life is rather short. Therefore, this study was aimed to evaluate the effect of inlet air temperature (T) and concentration (C) of maltodextrin and arabic gum on the spray-drying process of commercial camu-camu pulps (São Paulo and Manaus). Moisture, solubility, total phenolics (TP), ascorbic acid (AA), and proanthocyanidins (PAC) contents, and in vitro antioxidant capacity of the powders (FRAP, DPPH, Folin-Ciocalteu's reducing capacity were measured). Arabic gum resulted in better yields (22% to 30%), powder solubility (84% to 90%), and lower losses of analyzed compounds than the powders manufactured with maltodextrin. Overall, inlet air temperature had a lower impact on the responses studied than the concentration of carrier agents. Polynomial equations were generated for AA (R 2 = 0.993), TP (R 2 = 0.735), PAC (R 2 = 0.946), and for the antioxidant capacity assays (0.867 ≤ R 2  ≤ 0.963). In addition, principal component analysis showed that the lowest concentration of carrier agents (6%) in spray drying resulted in the lowest losses of bioactive compounds and, consequently, the highest antioxidant capacity. © 2017 Institute of Food Technologists®.

Efecto del Colgajo Desplazado Coronalmente con y sin Bioacondicionamiento Cementario de Myrciaria Dubia (Camu-Camu) en la Posición Gingival Aparente y Real en Pacientes con Recesión Gingival Clase II de Miller de la Clínica Odontológica. UCSM. Arequipa. 2015

OpenAIRE

Macedo Reynoso, Percy Antonio

2017-01-01

La presente investigación tiene por objeto determinar el efecto del colgajo desplazado coronalmente con y sin bioacondicionamiento cementario de Myrciaria Dubia en la posición gingival aparente y real en pacientes con recesión gingival clase II de Miller. Corresponde a un ensayo clínico, randomizado, por ende prospectivo, longitudinal, comparativo y de campo, de nivel cuasi-experimental. Las variables PGA y PGR han sido estudiadas por observación experimental clínica intraoral, una vez en...

The protective roles of TiO2 nanoparticles against UV-B toxicity in Daphnia magna.

Science.gov (United States)

Liu, Jie; Wang, Wen-Xiong

2017-09-01

Aquatic environments are increasingly under environmental stress due to ultraviolet (UV) radiation and potential inputs of nanoparticles with intense application of nanotechnology. In this study, we investigated the interaction between UV-B radiation and titanium nanoparticles (TiO 2 -NPs) in a model freshwater cladoceran Daphnia magna. UV-B toxicity to Daphnia magna was examined when the daphnids were exposed to a range of TiO 2 -NPs concentrations with an initial 5 or 10min of 200μW/cm 2 UV-B radiation. In addition, UV-B toxicity was also examined in the presence of TiO 2 -NPs in the body of daphnids. Our results demonstrated that the daphnid mortality under UV-B radiation decreased significantly in the presence of TiO 2 -NPs both in the water and in the body, indicating that TiO 2 -NPs had some protective effects on D. magna against UV-B. Such protective effect was mainly caused by the blockage of UV-B by TiO 2 -NPs adsorption. UV-B produced reactive oxygen species (ROS) in the water and in the daphnids, which was not sufficient to cause mortality of daphnids over short periods of radiation. Previous studies focused on the effects of TiO 2 -NPs on the toxicity of total UV radiation, and did not attempt to differentiate the potential diverse roles of UV-A and UV-B. Our study indicated that TiO 2 -NPs may conversely protect the UV-B toxicity to daphnids. Copyright © 2017 Elsevier B.V. All rights reserved.

Morphological and genetic diversity of camu-camu [Myrciaria dubia (Kunth McVaugh] in the Peruvian Amazon.

Directory of Open Access Journals (Sweden)

Jan Šmíd

Full Text Available Camu-camu [Myrciaria dubia (Kunth McVaugh] is currently an important and promising fruit species grown in the Peruvian Amazon, as well as in Brazil, Colombia, and Bolivia. The species is valued for its high content of fruit-based vitamin C. Large plantations have been established only in the last two decades, and a substantial part of the production is still obtained by collecting fruits from the wild. Domestication of the species is at an early stage; most farmers cultivate the plants without any breeding, or only through a simple mass selection process. The main objective of the study was to characterize morphological and genetic variation within and among cultivated and natural populations of camu-camu in the Peruvian Amazon. In total, we sampled 13 populations: ten wild in the Iquitos region, and three cultivated in the Pucallpa region in the Peruvian Amazon. To assess the genetic diversity using seven microsatellite loci, we analyzed samples from ten individual trees per each population (n = 126. Morphological data was collected from five trees from each population (n = 65. The analysis did not reveal statistically significant differences for most of the morphological descriptors. For wild and cultivated populations, the observed heterozygosity was 0.347 and 0.404 (expected 0.516 and 0.506, and the fixation index was 0.328 and 0.200, respectively. Wild populations could be divided into two groups according to the UPGMA and STRUCTURE analysis. In cultivated populations, their approximate origin was determined. Our findings indicate a high genetic diversity among the populations, but also a high degree of inbreeding within the populations. This can be explained by either the isolation of these populations from each other or the low number of individuals in some populations. This high level of genetic diversity can be explored for the selection of superior individuals for further breeding.

Morphological and genetic diversity of camu-camu [Myrciaria dubia (Kunth) McVaugh] in the Peruvian Amazon.

Science.gov (United States)

Šmíd, Jan; Kalousová, Marie; Mandák, Bohumil; Houška, Jakub; Chládová, Anna; Pinedo, Mario; Lojka, Bohdan

2017-01-01

Camu-camu [Myrciaria dubia (Kunth) McVaugh] is currently an important and promising fruit species grown in the Peruvian Amazon, as well as in Brazil, Colombia, and Bolivia. The species is valued for its high content of fruit-based vitamin C. Large plantations have been established only in the last two decades, and a substantial part of the production is still obtained by collecting fruits from the wild. Domestication of the species is at an early stage; most farmers cultivate the plants without any breeding, or only through a simple mass selection process. The main objective of the study was to characterize morphological and genetic variation within and among cultivated and natural populations of camu-camu in the Peruvian Amazon. In total, we sampled 13 populations: ten wild in the Iquitos region, and three cultivated in the Pucallpa region in the Peruvian Amazon. To assess the genetic diversity using seven microsatellite loci, we analyzed samples from ten individual trees per each population (n = 126). Morphological data was collected from five trees from each population (n = 65). The analysis did not reveal statistically significant differences for most of the morphological descriptors. For wild and cultivated populations, the observed heterozygosity was 0.347 and 0.404 (expected 0.516 and 0.506), and the fixation index was 0.328 and 0.200, respectively. Wild populations could be divided into two groups according to the UPGMA and STRUCTURE analysis. In cultivated populations, their approximate origin was determined. Our findings indicate a high genetic diversity among the populations, but also a high degree of inbreeding within the populations. This can be explained by either the isolation of these populations from each other or the low number of individuals in some populations. This high level of genetic diversity can be explored for the selection of superior individuals for further breeding.

Acute toxicity of fire control chemicals to Daphnia magna(Straus) and Selenastrum capricornutum(Printz)

Science.gov (United States)

McDonald, Susan F.; Hamilton, Steven J.; Buhl, Kevin J.; Heisinger, James F.

1996-01-01

Acute toxicity tests were conducted exposingDaphnia magnaStraus (daphnid) in soft and hard reconstituted waters (hardness 42 and 162 mg/liter as CaCO3, respectively), andSelenastrum capricornutumPrintz (algae) in ASTM algal assay medium (hardness 15 mg/liter as CaCO3) to fire retardants Fire-Trol GTS-R, Fire-Trol LCG-R, and Phos-Chek D75-F, and foam suppressants Phos-Chek WD-881 and Silv-Ex. The chemicals were slightly toxic to practically harmless to daphnids and moderately toxic to algae. Water quality did not consistently alter the toxicity of the test chemicals to daphnids. The most toxic chemical to daphnids was Silv-Ex (48-hr EC507 mg/liter in soft and hard waters), whereas the least toxic chemical to daphnids was Fire-Trol LCG-R (48-hr EC50848 mg/liter in soft water, 813 mg/liter in hard water). The most toxic chemical to algae was Fire-Trol LCG-R (96-hr IC5010 mg/liter), and the least toxic chemical was Phos-Chek D75-F (96-hr IC5079 mg/liter). Un-ionized ammonia concentrations near the EC50or IC50value in tests with the Fire-Trol compounds were frequently equal to or above reported LC50un-ionized ammonia concentrations. Un-ionized ammonia concentrations in tests with Phos-Chek D75-F were low, thus other toxic components present in the compounds probably contributed to the toxicity. When compared to the daphnids tested in ASTM soft water, the Fire-Trol compounds were most toxic to algae, whereas Phos-Chek D75-F and the foam suppressants were most toxic to daphnids. The results of these tests are comparable to those obtained from research conducted in other laboratories with the same species and similar chemicals. Accidental entry of fire-fighting chemicals into aquatic environments could adversely affect algae and aquatic invertebrates, thus disrupting ecosystem function.

Capacidad protectora de myrciaria dubia "camu camu" ante el daño genético inducido por estrés oxidativo, evaluado in vitro, en la línea celular de ovario de "hámster chino" cricetulus griseus e in vivo drosophila melanogaster “mosca de la fruta”

OpenAIRE

Gutierrez Bustamante, José Antonio

2008-01-01

It is well known that carcinogen and mutagens act through oxidatives mechanisms that they damage to the DNA, in this research we evaluate the protective capacity of Myrciaria dubia “camu camu” in vitro in an system constituted by cellular line CHO - K1 of ovary of hamster chinese Cricetulus griseus as well as in a system in vivo with Drosophila melanogaster. For test the genotoxicity and antigenotoxicity in vitro we used the aberrations chromosomal Test (AC) we used “camu camu” 3 doses (1,0;...

Acute and chronic toxicity of buprofezin on Daphnia magna and the recovery evaluation.

Science.gov (United States)

Liu, Yong; Qi, Suzhen; Zhang, Wen; Li, Xuefeng; Qiu, Lihong; Wang, Chengju

2012-11-01

The toxic effects of buprofezin on Daphnia magna after both chronic and acute exposures were evaluated according to OECD guidelines. A 48-h acute exposure of buprofezin resulted in daphnid immobility at an EC(50) of 0.44 mg/L. In a 14 days chronic exposure of buprofezin (0, 0.025, 0.05, 0.10 and 0.15 mg/L), the development and reproduction of daphnids were all significantly affected and the body length was more sensitive than other observed parameters. However, the adverse effects of buprofezin on parental daphnids can be passed on to their offspring and cannot be recovered in a short time.

Changes on physiological parameters of tambaqui (Colossoma macropomum) fed with diets supplemented with Amazonian fruit Camu camu (Myrciaria dubia).

Science.gov (United States)

Aride, P H R; Oliveira, A M; Batista, R B; Ferreira, M S; Pantoja-Lima, J; Ladislau, D S; Castro, P D S; Oliveira, A T

2018-05-01

The physiological responses of juvenile tambaqui (Colossoma macropomum) fed commercial feed supplemented with different concentrations of camu camu (Myrciaria dubia) were evaluated. The design was completely randomized, with treatments arranged in a factorial design with three proportions of camu camu (15%, 30% and 45%) and a control treatment (100% commercial diet), with four replicates per treatment. A total of 96 tambaqui specimens were used, with a mean initial weight of 11.69 ± 2.68 g and a mean length of 7.06 ± 0.44 cm. After 30 days, hematological parameters, metabolic variables, growth and fish swimming performance were evaluated. The different proportions of camu camu in the diet did not cause significant changes to the tambaqui's hematological parameters during the feeding period, except for hemoglobin (Hb) concentration and mean corpuscular hemoglobin concentration (MCHC) after the 30th day, and hematocrit (Ht) after the swimming stress test, which increased significantly (p < 0.05). The significant increases in metabolic variables, such as cortisol, glucose, proteins and triglycerides, and in hematologic variables after the Ucrit test reflect, respectively, biochemical adaptations for maintenance of the energy mobilization process and a regulatory necessity in tissue oxygen demand during intense exercise. Fish fed 15% and 30% camu camu gained the most weight and achieved the best swimming performance, respectively. The results for camu camu concentrations above 30% suggest a saturation of its intrinsic properties in the diet at this level and a loss of nutrients from the commercial feed replaced by the fruit, reducing productive performance and nutritional assimilation.

Changes on physiological parameters of tambaqui (Colossoma macropomum fed with diets supplemented with Amazonian fruit Camu camu (Myrciaria dubia

Directory of Open Access Journals (Sweden)

P. H. R. Aride

2017-09-01

Full Text Available Abstract The physiological responses of juvenile tambaqui (Colossoma macropomum fed commercial feed supplemented with different concentrations of camu camu (Myrciaria dubia were evaluated. The design was completely randomized, with treatments arranged in a factorial design with three proportions of camu camu (15%, 30% and 45% and a control treatment (100% commercial diet, with four replicates per treatment. A total of 96 tambaqui specimens were used, with a mean initial weight of 11.69 ± 2.68 g and a mean length of 7.06 ± 0.44 cm. After 30 days, hematological parameters, metabolic variables, growth and fish swimming performance were evaluated. The different proportions of camu camu in the diet did not cause significant changes to the tambaqui's hematological parameters during the feeding period, except for hemoglobin (Hb concentration and mean corpuscular hemoglobin concentration (MCHC after the 30th day, and hematocrit (Ht after the swimming stress test, which increased significantly (p < 0.05. The significant increases in metabolic variables, such as cortisol, glucose, proteins and triglycerides, and in hematologic variables after the Ucrit test reflect, respectively, biochemical adaptations for maintenance of the energy mobilization process and a regulatory necessity in tissue oxygen demand during intense exercise. Fish fed 15% and 30% camu camu gained the most weight and achieved the best swimming performance, respectively. The results for camu camu concentrations above 30% suggest a saturation of its intrinsic properties in the diet at this level and a loss of nutrients from the commercial feed replaced by the fruit, reducing productive performance and nutritional assimilation.

Uptake, retention and internalization of quantum dots in Daphnia is influenced by particle surface functionalization

Energy Technology Data Exchange (ETDEWEB)

Feswick, A., E-mail: afeswick@yahoo.ca [Center for Environmental and Human Toxicology, University of Florida, PO Box 110885, Gainesville, FL 32611 (United States); Canadian Rivers Institute, University of New Brunswick, PO Box 5050, Saint John NB, CA (United States); Griffitt, R.J., E-mail: joe.griffitt@usm.edu [Department of Coastal Sciences, University of Southern Mississippi, 703 East Beach Drive, Ocean Springs, MS 39564 (United States); Siebein, K., E-mail: kerry.siebein@nist.gov [Major Analytical Instrumentation Center, University of Florida, PO Box 116400, Gainesville, FL 32611 (United States); Barber, D.S., E-mail: barberd@vetmed.ufl.edu [Center for Environmental and Human Toxicology, University of Florida, PO Box 110885, Gainesville, FL 32611 (United States)

2013-04-15

Highlights: ► Daphnia underwent a waterborne exposure of PEG, NH{sub 2} and COOH functionalized quantum dot nanoparticles. ► There was preferential retention of COOH nanoparticles. ► TEM demonstrated that NH{sub 2} and COOH nanoparticles were internalized in cells adjacent to the GI tract. ► This cellular internalization was confirmed using energy dispersive spectroscopy. -- Abstract: Nanomaterials are a diverse group of compounds whose inevitable release into the environment warrants study of the fundamental processes that govern the ingestion, uptake and accumulation in aquatic organisms. Nanomaterials have the ability to transfer to higher trophic levels in aquatic ecosystems, and recent evidence suggests that the surface chemistry of both the nanoparticle and biological membrane can influence uptake kinetics. Therefore, our study investigates the effect of surface functionalization on uptake, internalization and depuration in Daphnia spp. Uncharged (polyethylene glycol; PEG), positively charged (amino-terminated: NH{sub 2}) and negatively charged (carboxyl-modified; COOH) cadmium selenide/zinc sulfide quantum dots were used to monitor ingestion, uptake and depuration of nanometals in Daphnia magna and Ceriodaphnia dubia over 24 h of exposure. These studies demonstrated that particles with higher negative charge (COOH quantum dots) were taken up to a greater extent by Daphnia (259.17 ± 17.70 RFU/20 Daphnia) than either the NH{sub 2} (150.01 ± 18.91) or PEG quantum dots (95.17 ± 9.78), however this is likely related to the functional groups attached to the nanoparticles as there were no real differences in zeta potential. Whole body fluorescence associates well with fluorescent microscopic images obtained at the 24 h timepoint. Confocal and electron microscopic analysis clearly demonstrated that all three types of quantum dots could cross the intestinal epithelial barrier and be translocated to other cells. Upon cessation of exposure, elimination of

Distinctive Architecture of the Chloroplast Genome in the Chlorodendrophycean Green Algae Scherffelia dubia and Tetraselmis sp. CCMP 881.

Science.gov (United States)

Turmel, Monique; de Cambiaire, Jean-Charles; Otis, Christian; Lemieux, Claude

2016-01-01

The Chlorodendrophyceae is a small class of green algae belonging to the core Chlorophyta, an assemblage that also comprises the Pedinophyceae, Trebouxiophyceae, Ulvophyceae and Chlorophyceae. Here we describe for the first time the chloroplast genomes of chlorodendrophycean algae (Scherffelia dubia, 137,161 bp; Tetraselmis sp. CCMP 881, 100,264 bp). Characterized by a very small single-copy (SSC) region devoid of any gene and an unusually large inverted repeat (IR), the quadripartite structures of the Scherffelia and Tetraselmis genomes are unique among all core chlorophytes examined thus far. The lack of genes in the SSC region is offset by the rich and atypical gene complement of the IR, which includes genes from the SSC and large single-copy regions of prasinophyte and streptophyte chloroplast genomes having retained an ancestral quadripartite structure. Remarkably, seven of the atypical IR-encoded genes have also been observed in the IRs of pedinophycean and trebouxiophycean chloroplast genomes, suggesting that they were already present in the IR of the common ancestor of all core chlorophytes. Considering that the relationships among the main lineages of the core Chlorophyta are still unresolved, we evaluated the impact of including the Chlorodendrophyceae in chloroplast phylogenomic analyses. The trees we inferred using data sets of 79 and 108 genes from 71 chlorophytes indicate that the Chlorodendrophyceae is a deep-diverging lineage of the core Chlorophyta, although the placement of this class relative to the Pedinophyceae remains ambiguous. Interestingly, some of our phylogenomic trees together with our comparative analysis of gene order data support the monophyly of the Trebouxiophyceae, thus offering further evidence that the previously observed affiliation between the Chlorellales and Pedinophyceae is the result of systematic errors in phylogenetic reconstruction.

Laboratory investigation into the development of resistance of Daphnia manga (straus) to environmental pollutants

Energy Technology Data Exchange (ETDEWEB)

LeBlanc, G.A.

1982-04-01

The purpose of this study was to determine the ability of Daphnia magna (Straus) to develop resistance to selected environmental pollutants through physiological adaptation or the selection of genetically fit organisms. The results of the study demonstrated that daphnids pre-exposed to copper, lead or zinc for 20 h had become significantly resistant to the toxic effects of these metals. Similar tests performed with sodium lauryl sulfate, however, resulted in no increased resistance. Exposure of successive generations of daphnids to copper resulted in the development of significant resistance to copper. However, this resistance was determined to be the result of physiological adaptation and was not due to the selection of genetically fit organisms. Multigeneration exposures to sodium lauryl sulfate failed to produce any increased resistance to the toxic effects of this compound.Exposure of successive generations to sodium lauryl sulfate increased each generation's sensitivity to the toxicity of this compound. To determine if resistance to one pollutant might confer resistance to others, the resistance, to zinc and lead, of daphnids pre-exposed to copper was assessed. Copper-resistant daphnids demonstrated no increased resistance to the toxic effects of zinc or lead.

Changes in the metabolic elimination profile of testosterone following exposure of the crustacean Daphnia magna to tributyltin.

Science.gov (United States)

LeBlanc, G A; McLachlan, J B

2000-03-01

The biocide tributyltin has been found to cause the development of pseudohermaphroditic conditions in some neogastropod species. These abnormalities of the reproductive system have adversely affected the fecundity of some field populations of gastropods, resulting in local population declines. Current evidence suggests that tributyltin elicits these effects by interfering with the biotransformation of testosterone to other steroid derivatives, resulting in an elevation in endogenous testosterone or some of its bioactive derivatives. The purpose of the present study was to determine whether tributyltin altered testosterone metabolism in daphnids (Daphnia magna), a species commonly used in ecotoxicology testing. Exposure of daphnids to 1.2 microg (tin)/L caused a general increase in the rate of elimination of oxido-reduced, hydroxylated, and glucose-conjugated derivatives of testosterone. However, tributyltin exposure had no significant effect on the rate of elimination of the glucose-conjugated forms of the various oxido-reduced and hydroxylated derivatives of testosterone. As a result, the percentage of the oxido-reduced and hydroxylated metabolites of testosterone eliminated as glucose conjugates decreased with increasing tributyltin exposure levels. These results demonstrate that tributyltin causes alterations in testosterone metabolism in daphnids that would result in an increase in the production of oxido-reduced derivatives. These products are preferentially retained in the tissues of daphnids and are variously androgenic in vertebrates. The increased production of oxido-reduced derivatives of testosterone may be mechanically responsible for the masculinizing effects of tributyltin in some species and suggests that daphnids may be a suitable surrogate for evaluating the potential of chemicals to elicit this form of toxicity. Copyright 2000 Academic Press.

Relationship between the energy status of Daphnia magna and its sensitivity to environmental stress

International Nuclear Information System (INIS)

Smolders, Roel; Baillieul, Marc; Blust, Ronny

2005-01-01

This work tested the hypothesis that animals with a high energy status are more successful in dealing with stress than animals with a low energy status. Daphnids (Daphnia magna) were reared for 2 weeks in four different concentrations of food. Survival was not affected by food supply, and growth and reproduction increased with increasing food ration. This increase correlated well with the energy status, as was measured by scope for growth on day 15. After 2 weeks, the daphnids in the four different food ration groups were exposed for another 2 weeks to a range of increased salinities or cadmium concentrations, while remaining in their respective food concentrations. In the salinity groups, survival, growth, or reproduction were not influenced at low salinities. Exposure to higher salinity significantly decreased survival and reproduction, but this decrease was more pronounced in the highest food concentrations. In the cadmium exposed daphnids, cadmium content increased with increasing exposure concentrations, but accumulation was independent of food rations. Cadmium exposure significantly decreased survival, growth, and reproduction and this decrease again was more pronounced with increasing food concentration. Thus, the high energy status of the daphnids from the high food concentrations at the start of the exposure did not provide an increased capacity to cope with additional stress. Instead, the sensitivity of the daphnids to stress increased with increasing food ration. This increased sensitivity is likely to be the result of a change in life history from emphasizing survival at low food supply to stressing reproduction at high food supply

Possible factors which produce fruit drop of Myrciaria dubia (HBK Mc Vaugh, "camu camu" during the reproductive phenology in the collection “cinco cuencas” from the San Miguel experimental centerIIAP, Loreto, Peru

Directory of Open Access Journals (Sweden)

Sonia Farro

2010-06-01

Full Text Available "Camu camu" plants were evaluated in five basins in Loreto (Peru, according to the percentage of fruits with symptoms of pest infestation, retention of flowers and fruits in each basin, diameters of branches, and the influence of precipitation and temperature in the process of falling fruit as the phenological stage. In the genetic factor, the Putumayo river basin highlighted by a longer retention of fruit, higher yield and average fruit weight and less attack by pests. During the phenological process that lasted 12 weeks, the critical stage of flower and fruit drop occurred during the first seven, with the retention of flowers of 5.12%. The pests are causing the observed fall 9.27%, 9.15% being caused by Edessa sp., And 0.12% for Conotrachellus dubiae. The other 90.73% was caused by other undetermined factors such as physiological, nutritional, competition, wind, rain. Environmental factors of temperature and precipitation have a direct influence and inversely proportional to the fall of fruits, respectively.

Microplastics Reduce Short-Term Effects of Environmental Contaminants. Part I: Effects of Bisphenol A on Freshwater Zooplankton Are Lower in Presence of Polyamide Particles

Science.gov (United States)

Rehse, Saskia; Kloas, Werner; Zarfl, Christiane

2018-01-01

Microplastics can have direct physical effects on organisms in freshwater systems, and are considered as vectors for absorbed environmental pollutants. It is still under discussion if microplastics are relevant pollutant vectors for uptake into aquatic organisms in comparison to further uptake pathways, e.g., via water or sediment particles. We analyzed how the presence of microplastics (polyamide particles, PA) modifies acute effects of the environmental pollutant bisphenol A (BPA) on freshwater zooplankton (Daphnia magna). Daphnids were exposed to PA particles and BPA alone, before combining them in the next step with one concentration of PA and varying concentrations of BPA. The PA particles themselves did not induce negative effects, while the effects of BPA alone followed a typical dose-dependent manner. Sorption of BPA to PA particles prior to exposure led to a reduction of BPA in the aqueous phase. The combination of BPA and PA led to decreased immobilization, although PA particles loaded with BPA were ingested by the daphnids. Calculations based on physiochemistry and equilibrium assumptions indicated lower BPA body burden of daphnids in the presence of PA particles. These results confirm model-based studies, and show that investigated microplastic concentrations are negligible for the overall pollutant uptake of daphnids with water as additional uptake pathway. PMID:29415519

Microplastics Reduce Short-Term Effects of Environmental Contaminants. Part I: Effects of Bisphenol A on Freshwater Zooplankton Are Lower in Presence of Polyamide Particles

Directory of Open Access Journals (Sweden)

Saskia Rehse

2018-02-01

Full Text Available Microplastics can have direct physical effects on organisms in freshwater systems, and are considered as vectors for absorbed environmental pollutants. It is still under discussion if microplastics are relevant pollutant vectors for uptake into aquatic organisms in comparison to further uptake pathways, e.g., via water or sediment particles. We analyzed how the presence of microplastics (polyamide particles, PA modifies acute effects of the environmental pollutant bisphenol A (BPA on freshwater zooplankton (Daphnia magna. Daphnids were exposed to PA particles and BPA alone, before combining them in the next step with one concentration of PA and varying concentrations of BPA. The PA particles themselves did not induce negative effects, while the effects of BPA alone followed a typical dose-dependent manner. Sorption of BPA to PA particles prior to exposure led to a reduction of BPA in the aqueous phase. The combination of BPA and PA led to decreased immobilization, although PA particles loaded with BPA were ingested by the daphnids. Calculations based on physiochemistry and equilibrium assumptions indicated lower BPA body burden of daphnids in the presence of PA particles. These results confirm model-based studies, and show that investigated microplastic concentrations are negligible for the overall pollutant uptake of daphnids with water as additional uptake pathway.

Growth of floating-leaved and submerged plants in artificial co-cultured microcosms: morphological responses to various water fluctuation regimes

International Nuclear Information System (INIS)

Cao, Q.J.

2015-01-01

Hydrocharis dubia can alternate between a rooted floating-leaved and a free-floating form, so given increasingly frequent precipitation extremes, it is not expected to be more negatively impacted by rapid water fluctuations than by gradual ones and may adapt water fluctuations by alteration of life forms. However, the opposite may be true for Nymphoides peltata, with only a rooted floating-leaved form. We designed an experiment combining six water depth treatments (constant shallow, constant deep, and two rapidly and two gradually fluctuating treatments) with three speciescombinations (N. peltata H. dubia, N. peltata Ceratophyllum demersum, and H. dubia C. demersum) to investigate plant responses to depth fluctuations and their co-cultured species. The total mass of N. peltata was considerably lower in the rapidly- than in the gradually-fluctuating treatments. However, total mass of H. dubia in the rapidly-fluctuating treatments was similar to or higher than in the gradually-fluctuating ones. Rapid fluctuations had a negative impact on the growth of C. demersum than gradual fluctuating. The floating-leaved species demonstrated divergent adaptive strategies to different water fluctuation patterns. In addition to expanding leaf blades, H. dubia can adapt to changing water depths by changing its life form. However, N. peltata, which mainly relies on morphological plasticity, such as petiole elongation, to adapt to water rise may reduce its abundance in communities subjected to increasingly frequent floods. The growth of submerged C. demersum, either co-occurring with H. dubia or with N. peltata, may be repressed by high flooding rates. (author)

Semillas y plántulas de Myrciaria dubia “camu-camu”: biometría, germinación y crecimiento inicial

Directory of Open Access Journals (Sweden)

Anderson E. Medina Bardales

2014-01-01

Full Text Available Los objetivos planteados fueron determinar la biometría de las semillas, registrar la respiración celular en el proceso de imbibición, evaluar el efecto inductor de la germinación de compuestos químicos y analizar el crecimiento inicial. La respiración celular durante la imbibición se midió por reducción del azul de metileno y del Janus Green B. El efecto inductor de la germinación de cinco compuestos químicos fue evaluado. El crecimiento inicial se registró con estereoscopio. Los resultados mostraron que las semillas tienen en promedio un peso de 520±172 mg, largo de 13,50±1,76 mm, ancho de 10,61±1,14 mm y espesor de 4,68±0,62 mm. Estas variables mostraron correlaciones significativas (r de 0,494 a 0,897 y p 90% y 65% respectivamente. El crecimiento hasta plántula constó de seis etapas y ocurrió en un periodo de 22 días después del brote de la radícula. En conclusión, las semillas de M. dubia muestran una amplia variación en sus datos biométricos (peso, largo, ancho y espesor, pero muestran correlaciones significativas entre sí. Asimismo, las semillas son metabólicamente activas en el proceso de imbibición y su germinación es inducida por el nitrito de sodio y el cianuro de potasio. Además, el crecimiento inicial hasta plántula ocurre en un periodo de 22 días después del brote de la radícula y consta de seis etapas bien diferenciadas.

Integral Monitored Retrievable Storage (MRS) Facility conceptual design report

International Nuclear Information System (INIS)

1985-09-01

In April 1985, the Department of Energy (DOE) selected the Clinch River site as its preferred site for the construction and operation of the monitored retrievable storage (MRS) facility (USDOE, 1985). In support of the DOE MRS conceptual design activity, available data describing the site have been gathered and analyzed. A composite geotechnical description of the Clinch River site has been developed and is presented herein. This report presents Clinch River site description data in the following sections: general site description, surface hydrologic characteristics, groundwater characteristics, geologic characteristics, vibratory ground motion, surface faulting, stability of subsurface materials, slope stability, and references. 48 refs., 35 figs., 6 tabs

Efecto citoprotector del camu-camu Myrciaria dubia en tres líneas celulares de ratón expuestos in vivo a bromato de potasio

Directory of Open Access Journals (Sweden)

Alvis Rafael

2011-05-01

Full Text Available Se evaluó in vivo la capacidad citoprotectora del fruto de Myrciaria dubia (Kunth McVaugh Camu-camu frente al daño mutagénico producido por bromato de potasio (68,5 mg/k sobre tres líneas celulares de ratón (hígado, riñón y células sanguíneas. Se utilizó ratones (n= 120 divididos en tres grupos los cuales bebieron ad libitum: TI= control negativo (solo agua y el grupo TIII (control positivo; El grupo TII bebió el extracto acuoso (2% p/v del fruto de camu-camu. A los diez días se inyectó una dosis única de KBr03 (68,5 mg/kg peso corporal vía intraperitoneal, a los grupos TII y TIII. El tratamiento con camu-camu continuo 35 días más, luego los ratones fueron eutanizados para determinar la frecuencia del daño al DNA mediante el protocolo del ensayo cometa alcalino. El grupo TII mostró en todas las líneas celulares el efecto citoprotector del camu-camu (p< 0,05. El efecto dañino al DNA por la acción oxidativa del KBrO3 es inhibido por el extracto acuoso del fruto de camu camu, probablemente por la presencia de los agentes antioxidantes como el Acido ascórbico y los flavonoides.

Tributyltin synergizes with 20-hydroxyecdysone to produce endocrine toxicity.

Science.gov (United States)

Wang, Ying H; Kwon, Gwijun; Li, Hong; Leblanc, Gerald A

2011-09-01

One of the great challenges facing modern toxicology is in predicting the hazard associated with chemical mixtures. The development of effective means of predicting the toxicity of chemical mixtures requires an understanding of how chemicals interact to produce nonadditive outcomes (e.g., synergy). We hypothesized that tributyltin would elicit toxicity in daphnids (Daphnia magna) by exaggerating physiological responses to 20-hydroxyecdysone signaling via synergistic activation of the retinoid X receptor (RXR):ecdysteroid receptor (EcR) complex. Using reporter gene assays, we demonstrated that RXR, alone, is activated by a variety of ligands including tributyltin, whereas RXR:EcR heterodimers were not activated by tributyltin. However, tributyltin, in combination with the daphnid EcR ligand 20-hydroxyecdysone, caused concentration-dependent, synergistic activation of the RXR:EcR reporter. Electrophoretic mobility shift assays revealed that tributyltin did not enhance the activity of 20-hydroxyecdysone by increasing binding of the receptor complex to a DR-4 DNA-binding site. Exposure of daphnids to elevated concentrations of 20-hydroxyecdysone caused premature and incomplete ecdysis resulting in death. Tributyltin exaggerated this effect of exogenous 20-hydroxyecdysone. Further, exposure of daphnids to tributyltin enhanced the inductive effects of 20-hydroxyecdysone on expression of the 20-hydroxyecdysone-inducible gene HR3. Continuous, prolonged exposure of maternal daphnids to concentrations of tributyltin resulted in mortality concurrent with molting. Taken together, these results demonstrate that xenobiotics, such as tributyltin, can interact with RXR to influence gene expression regulated by the heterodimeric partner to RXR. The result of such interactions can be toxicity due to inappropriate or exaggerated hormonal signaling. The application of the in vitro/in vivo approach used in this study is discussed in relation to modeling of nonadditive interactions

Irrigation runoff insecticide pollution of rivers in the Imperial Valley, California (USA)

Energy Technology Data Exchange (ETDEWEB)

Vlaming, V. de [Aquatic Toxicology Laboratory, VM: APC, 1321 Haring Hall, University of California, Davis, CA 95616 (United States)]. E-mail: vldevlaming@ucdavis.edu; DiGiorgio, C. [Department of Water Resources, P.O. Box 942836, Sacramento, CA 94236 (United States); Fong, S. [Aquatic Toxicology Laboratory, VM: APC, 1321 Haring Hall, University of California, Davis, CA 95616 (United States); Deanovic, L.A. [Aquatic Toxicology Laboratory, VM: APC, 1321 Haring Hall, University of California, Davis, CA 95616 (United States); Paz Carpio-Obeso, M. de la [Colorado River Basin Region Water Quality Control Board, 73-720 Fred Waring Drive, Suite 100, Palm Desert, CA 92260 (United States); Miller, J.L. [AQUA-Science, 17 Arboretum Drive, Davis, CA 95616 (United States); Miller, M.J. [AQUA-Science, 17 Arboretum Drive, Davis, CA 95616 (United States); Richard, N.J. [Division of Water Quality, State Water Resources Control Board, 1001 I Street, Sacramento, CA 95814 (United States)

2004-11-01

The Alamo and New Rivers located in the Imperial Valley, California receive large volumes of irrigation runoff and discharge into the ecologically sensitive Salton Sea. Between 1993 and 2002 we conducted a series of studies to assess water quality using three aquatic species: a cladoceran (Ceriodaphnia dubia), a mysid (Neomysis mercedis), and a larval fish (Pimephales promelas). Although no mortality was observed with the P. promelas, high-level toxicity to the invertebrate species was documented in samples from both rivers during many months of each year. Toxicity identifications and chemical analyses identified the organophosphorus insecticides (OP), chlorpyrifos and diazinon, as the cause of C. dubia toxicity. The extent of the C. dubia mortality was highly correlated with quantities of these OPs applied in the river watersheds. C. dubia mortality occurred during more months of our 2001/2002 study than in the 1990s investigations. During 2001/2002, the extensive C. dubia mortality observed in New River samples was caused by OP insecticide pollution that originated from Mexico. Mortality to N. mercedis in New River samples was likely caused by contaminants other than OP insecticides. Our studies document OP insecticide-caused pollution of the Alamo River over a 10-year period and provide the necessary information for remediation efforts. - Capsule: Organophosphorous insecticides in runoff water from the USA and Mexico have impacted rivers in the Imperial Valley, California.

Irrigation runoff insecticide pollution of rivers in the Imperial Valley, California (USA)

International Nuclear Information System (INIS)

Vlaming, V. de; DiGiorgio, C.; Fong, S.; Deanovic, L.A.; Paz Carpio-Obeso, M. de la; Miller, J.L.; Miller, M.J.; Richard, N.J.

2004-01-01

The Alamo and New Rivers located in the Imperial Valley, California receive large volumes of irrigation runoff and discharge into the ecologically sensitive Salton Sea. Between 1993 and 2002 we conducted a series of studies to assess water quality using three aquatic species: a cladoceran (Ceriodaphnia dubia), a mysid (Neomysis mercedis), and a larval fish (Pimephales promelas). Although no mortality was observed with the P. promelas, high-level toxicity to the invertebrate species was documented in samples from both rivers during many months of each year. Toxicity identifications and chemical analyses identified the organophosphorus insecticides (OP), chlorpyrifos and diazinon, as the cause of C. dubia toxicity. The extent of the C. dubia mortality was highly correlated with quantities of these OPs applied in the river watersheds. C. dubia mortality occurred during more months of our 2001/2002 study than in the 1990s investigations. During 2001/2002, the extensive C. dubia mortality observed in New River samples was caused by OP insecticide pollution that originated from Mexico. Mortality to N. mercedis in New River samples was likely caused by contaminants other than OP insecticides. Our studies document OP insecticide-caused pollution of the Alamo River over a 10-year period and provide the necessary information for remediation efforts. - Capsule: Organophosphorous insecticides in runoff water from the USA and Mexico have impacted rivers in the Imperial Valley, California

Evaluation of Daphnia ambigua for Routine Aquatic Toxicity Testing at the Savannah River Site

International Nuclear Information System (INIS)

Specht, W.L.; Harmon, S.M.

1997-09-01

Short-term whole effluent toxicity testing, which is currently a requirement of the U.S. EPA's National Pollution Discharge Elimination System (NPDES), commonly uses the cladoceran species Ceriodaphnia dubia. Despite the advantages to using a common test species to model the toxic effects of effluents, it could be argued that toxicity test results would be more meaningful if a wider variety of test organisms were commonly used. One particular argument against C. dubia is that tests conducted with this species do not always reflect local, site-specific conditions. The careful selection and use of an indigenous test species would produce a more realistic model of local instream effects and would account for regional differences in water quality. Permitted effluent discharges from Savannah River Site (SRS), a government weapons facility operated by the U.S. Department of Energy, require toxicity testing with C. dubia. However, water quality in these receiving streams is markedly different (lower pH and hardness) from standard laboratory water used for the culturing and testing of C. dubia, and it has been shown that this receiving water presents varying degrees of toxicity to C. dubia. Based on these results, it is possible that toxic effects observed during an effluent study could be the result of test organism stress from the dilution water and not the effects of SRS effluents. Therefore, this study addressed the substitution of C. dubia with an indigenous cladoceran species, Daphnia ambigua for routine regulatory testing at SRS. Given the indigenous nature of this species, combined with the fact that it has been successfully cultured by other investigators, D. ambigua was ideal for consideration as a replacement for C. dubia, but further study of the overall success and sensitivity of laboratory-reared D. ambigua was required. This investigation determined that D. ambigua could be laboratory cultured with only minimal changes to established regulatory protocol and

Uptake and effects of microplastic textile fibers on freshwater crustacean Daphnia magna.

Science.gov (United States)

Jemec, Anita; Horvat, Petra; Kunej, Urban; Bele, Marjan; Kržan, Andrej

2016-12-01

Microplastic fibers (MP) from textile weathering and washing are increasingly being recognized as environmental pollutants. The majority of studies on the bioavailability and effects of microplastic focused on small polystyrene spherical plastic particles, while less data are available for fibers and for other materials besides polystyrene. We investigated the ingestion and effects of ground polyethylene terephthalate (PET) textile microfibers (length range: 62-1400 μm, width 31-528 μm, thickness 1-21.5 μm) on the freshwater zooplankton crustacean Daphnia magna after a 48 h exposure and subsequent 24 h of recovery in MP free medium and algae. The majority of ingested fibers by D. magna were around 300 μm, but also some very large twisted MP fibers around 1400 μm were found inside the gut. Exposure to these fibers results in increased mortality of daphnids after 48 h only in the case where daphnids were not pre-fed with algae prior to experiment, but no effect was found when daphnids were fed before the experiments. Regardless of the feeding regime, daphnids were not able to recover from MP exposure after additional 24 h incubation period in a MP free medium with algae. The uptake and effects of PET textile MP on D. magna are presented here for the first time. Copyright © 2016 Elsevier Ltd. All rights reserved.

Assessing caffeine as an emerging environmental concern using conventional approaches.

Science.gov (United States)

Moore, M T; Greenway, S L; Farris, J L; Guerra, B

2008-01-01

Organic wastewater contaminants, including pharmaceuticals, caffeine, and nicotine, have received increased scrutiny because of their detection in water bodies receiving wastewater discharge. Despite recent measurement in United States streams, caffeine's effect on freshwater organisms is not well documented. The present study measured caffeine's lethal and sublethal effects on the freshwater species, Ceriodaphnia dubia, Pimephales promelas, and Chironomus dilutus. These organisms, which are used in standard testing or effluent monitoring, were exposed to aqueous caffeine solutions under static exposure for 48 hours and daily renewed static exposure for 7 days. Averaged responses of 48-hour acute end points indicated that C. dubia was more sensitive to caffeine exposures (LC50 = 60 mg/L) than either P. promelas (LC50 = 100 mg/L) or C. dilutus (LC50 = 1,230 mg/L). Exposure-response slopes confirmed these findings (3% mortality/mg/L for C. dubia; 0.5% mortality/mg/L for P. promelas; and 0.07% mortality/mg/L for C. dilutus). Comparative 7-day responses between C. dubia and P. promelas (LC50 = 46 and 55 mg/L, respectively) were more similar than the broad range of acute values. Sublethal effects measured for caffeine exposure included impaired C. dubia reproduction (IC50 = 44 mg/L) and inhibited P. promelas growth (IC50 = 71 mg/L). According to the results of this study, combined with earlier studies reporting environmental concentrations and product half-lives, caffeine should pose negligible risk for most aquatic vertebrate and invertebrate organisms.

Eficacia tópica de Myrciaria dubia en la curación de quemaduras de segundo grado en ratas Holtzman.

Directory of Open Access Journals (Sweden)

Karla Pacci-Salazar

2009-01-01

Full Text Available Objetivo, Comparar el efecto de la crema a base de Myrciaria dubia con el efecto antibiótico de la crema de sulfadiazina argéntica. Diseño, El presente es un estudio experimental aleatorizado incompleto. Participantes, 15 ratas Holtzman hembras de 9-10 semanas a las cuales se les produjo quemaduras de segundo grado. Intervenciones, Se produjo una quemadura cuadrada de 1cm2 de piel del dorso de las ratas usando un cautín modificado para producir tres quemaduras térmicas por cada animal. Se seleccionó aleatoriamente la herida para cada tratamiento. Principales medidas de resultados, La crema a base de camu-camu se elabora con una extracción etanólica al 5% para mezclarla con Sepigel 350® a una concentración de 5%. Las heridas recibieron administración cada doce horas durante cinco días. Resultados, El promedio de la reducción de la cicatriz en el grupo camu-camu es de 69,4 ± 52,85, mientras que en el grupo sulfadiazina argéntica es de 69,26 ± 53,66. Microscópicamente se observó similar infiltración leucocitaria en la dermis y en el estrato seroso en los grupos camu-camu y sulfadiazina argéntica, y ambas fueron menores que en la del grupo control. Utilizando la prueba ANOVA, se observó que, a pesar de que la cantidad de fibroblastos en el grupo camu-camu era ligeramente mayor que en la de sulfadiazina argéntica, la diferencia no era estadísticamente significativa. Conclusiones, La presencia de epidermis en el grupo Camu-Camu, a diferencia de los demás grupos, se puede deber a una mayor de activación de células basales o a una detención de los procesos oxidativos debido a la propiedad antioxidante de este fruto.

Fluctuación y distribución espacio-temporal de Tuthillia cognata (Hemiptera, Psyllidae y de Ocyptamus persimilis (Diptera, Syrphidae en el cultivo de camu-camu Myrciaria dubia (Myrtaceae en Ucayali, Perú Fluctuation and temporal-spatial distribution of Tuthillia cognata (Hemiptera, Psyllidae and Ocyptamus persimilis (Diptera, Syrphidae on camu-camu Myrciaria dubia (Myrtaceae in Ucayali, Peru

Directory of Open Access Journals (Sweden)

Diana Pérez

2009-12-01

Full Text Available Tuthillia cognata Hodkinson, Brown & Burckhardt, 1986 (Hemiptera, Psyllidae es una plaga importante en el cultivo de camu-camu, Myrciaria dubia H.B.K. Mc Vaugh (Myrtaceae en la Amazonía Peruana. El objetivo del presente estudio fue determinar la fluctuación y la distribución espacio-temporal de T. cognata y de su controlador biológico Ocyptamus persimilis (Curran, 1930 (Diptera, Syrphidae, entre enero a noviembre del 2004 en los caseríos San Juan y Padre Bernardo, Pucallpa, Ucayali, Perú. El número de ninfas y adultos de T. cognata fue mayor en la época lluviosa que en la seca, pero no en el número de huevos, ni en el número de colonias. Los huevos, ninfas y adultos prefirieron el tercio superior de la planta en comparación con el tercio medio e inferior en ambos caseríos. La excepción fue la presencia de huevos en el caserío San Juan que no mostró preferencias por ninguno de los tercios. No se observaron diferencias en el porcentaje de infestación por T. cognata entre ambos caseríos y entre la época seca y lluviosa. En O. persimilis, no se encontró diferencias en el número de huevos, larvas y pupas entre la época seca y lluviosa. Se observó que los huevos, larvas y pupas prefirieron el tercio superior de la planta en comparación con el tercio medio e inferior en ambos caseríos. Ambas especies, T. cognata y O. persimilis presentaron un patrón de distribución espacial - temporal agregado. Se encontró una relación directa entre el número de colonias de T. cognata y O. persimilis. Sin embargo, el número de huevos, ninfas y adultos de T. cognata no se encontraron correlacionados con el número de huevos, larvas y pupas de O. persimilis.Tuthillia cognata Hodkinson, Brown & Burckhardt, 1986 (Hemiptera, Psyllidae, is a main pest on camu-camu culture Myrciaria dubia H.B.K. Mc Vaugh (Myrtaceae in the Peruvian Amazon. The aim of the current research was to evaluate the fluctuation and spatial and temporal distribution of T

Avances de investigación para la propagación vegetativa por cultivo in vitro de Myrciaria dubia (H.B.K. Mc Vaugh “camu camu”

Directory of Open Access Journals (Sweden)

Sergio Pinedo Freyre

2013-06-01

Full Text Available El trabajo fue realizado en el Laboratorio de Cultivo de Tejidos Vegetales de la Estación Experimental Agraria San Roque, del INIA Loreto con el objetivo de establecer un protocolo para el cultivo in vitro de segmentos nodales de camu camu" Myrciaria dubia " (H.B.K Mc Vaugh. La fuente de material vegetal, fueron ramas de la parte apical de plantas adultas de la Colección Nacional de camu camu del INIA Loreto, se realizaron ensayos de desinfección, medios de cultivo y diferentes concentraciones de hormonas. El medio de cultivo utilizado fue Murashige & Skoog, MS 1962; más factores constantes 20 g/L sacarosa, 0,25 g/L carbón activado, 7 g/L agar y pH 5,7. Los tratamientos de desinfección que presentaron los mayores porcentajes (100% de supervivencia de segmentos nodales, fueron: Hipoclorito 0,3% + Chupadera 1 g/L en M&S, M&S ½ y M&S + AIB y Hipoclorito 0,5% + Chupadera 2 g/L en M&S, M&S ½ y M&S + AIB. El mejor medio de cultivo para la fase de crecimiento in vitro fue M&S + 1,5 mg/L GA3 + 10 mg/L AIA con 72% de supervivencia a los 45 días después de la siembra. El mejor medio de cultivo para la fase de multiplicación fue M&S + 2,5 mg/L BAP + 0,1 mg/L ANA con 80% de supervivencia a los 30 días después del subcultivo.

Teores de nutrientes e fertirrigação em cultivo de camu-camu (Myrciaria dubia (Kunth McVaugh com diferentes doses de nitrogênio

Directory of Open Access Journals (Sweden)

Carlos Abanto Rodriguez

2018-01-01

Full Text Available O camu-camu (Myrciaria dubia (Kunth McVaugh é uma fruteira nativa que está em processo de domesticação, portanto, o conhecimento das necessidades nutricionais é essencial para melhorar a eficiência da adubação durante seu desenvolvimento. O trabalho teve como objetivo determinar os teores de nutrientes em plantas de camu-camu em função da adubação nitrogenada via fertirrigação no primeiro ano de cultivo. O experimento foi conduzido mediante delineamento experimental em blocos casualizados, com 5 tratamentos: 0; 40; 80; 120 e 160 kg ha-1 de N, oito repetições e sete plantas por unidade experimental. As variáveis avaliadas foram a massa seca total e os teores de nutrientes nas folhas. Os resultados mostram que a massa seca total das plantas de camu-camu apresentou comportamento quadrático significativo em resposta às doses crescentes de N, sendo que doses maiores a 128 kg ha-1 propiciaram efeitos depressivos. Além disso, os teores foliares de N, K, Ca tiveram uma resposta quadrática significativa, sendo que as maiores concentrações foram registradas entre as doses de 113 e 115 kg de N ha-1. Por outro lado, os micronutrientes tiveram um comportamento polinomial depressivo de acordo com os incrementos das doses de N. Pode-se concluir que os teores foliares dos nutrientes estão dentro dos adequados para o desenvolvimento das plantas de camu-camu, e os nutrientes mais exigidos foram o N, Ca, K, Fe, Mn e B, e finalmente os teores de macro e micronutrientes determinados nas folhas responderam a seguinte ordem decrescente: N > Ca > K > Mg > S > P > Mn >Fe > B > Zn > Cu, respectivamente.

Analysis of the swimming velocity of cadmium-stressed Daphnia magna

International Nuclear Information System (INIS)

Baillieul, M.; Blust, R.

1999-01-01

The swimming velocity of the waterflea Daphnia magna is dependent on its body size. Therefore, environmental factors like toxic stress that influence growth also influence swimming velocity. An experiment was set up to test whether exposure to cadmium would reduce only growth, with a concomitant decrease in velocity, or whether it would reduce velocity below the swimming velocity of similarly-sized control animals. Daphnids were exposed for 10 days to free cadmium ion concentrations ranging from 1x10 -8 to 1x10 -7 M Cd 2+ , and body size and swimming velocity were measured every 2 days. The results showed that cadmium decreased both growth and velocity, i.e. exposed daphnids swam slower than similarly-sized control daphnids. Swimming velocity provided no indication of successful acclimation in any cadmium treatment. Food consumption and assimilation were reduced by exposure to cadmium. This reduced food intake may have, at least partially, caused the decreased growth rates. However, since reduced food intake does not affect swimming velocity, the reduced swimming velocity must be attributed to toxic effects of cadmium, other than those on food intake. (Copyright (c) 1999 Elsevier Science B.V., Amsterdam. All rights reserved.)

Avances de investigación para la propagación vegetativa por cultivo in vitro de Myrciaria dubia (H.B.K. Mc Vaugh “camu camu”

Directory of Open Access Journals (Sweden)

Sergio Pinedo Freyre

2013-06-01

Full Text Available El trabajo fue realizado en el Laboratorio de Cultivo de Tejidos Vegetales de la Estación Experimental Agraria San Roque, del INIA Loreto con el objetivo de establecer un protocolo para el cultivo in vitro de segmentos nodales de camu camu" Myrciaria dubia " (H.B.K Mc Vaugh. La fuente de material vegetal, fueron ramas de la parte apical de plantas adultas de la Colección Nacional de camu camu del INIA Loreto, se realizaron ensayos de desinfección, medios de cultivo y diferentes concentraciones de hormonas. El medio de cultivo utilizado fue Murashige & Skoog, MS 1962; más factores constantes 20 g/L sacarosa, 0,25 g/L carbón activado, 7 g/L agar y pH 5,7. Los tratamientos de desinfección que presentaron los mayores porcentajes (100% de supervivencia de segmentos nodales, fueron: Hipoclorito 0.3%  + Chupadera 1 g/L en M&S, M&S ½ y M&S + AIB y Hipoclorito 0,5%  + Chupadera 2 g/L en M&S, M&S ½ y M&S + AIB. El mejor medio de cultivo para la fase de crecimiento in vitro fue M&S + 1,5 mg/L GA3 + 10 mg/L AIA con 72% de supervivencia a los 45 días después de la siembra. El mejor medio de cultivo para la fase de multiplicación fue M&S + 2,5 mg/L BAP + 0,1 mg/L ANA con 80% de supervivencia a los 30 días después del subcultivo.

Transmission of Leishmania infantum in the Canine Leishmaniasis Focus of Mont-Rolland, Senegal: Ecological, Parasitological and Molecular Evidence for a Possible Role of Sergentomyia Sand Flies.

Directory of Open Access Journals (Sweden)

Massila Wagué Senghor

2016-11-01

Full Text Available Leishmania (L. infantum is the causative agent in an endemic focus of canine leishmaniasis in the Mont-Rolland district (Thiès, Senegal. In this area, the transmission cycle is well established and more than 30% of dogs and 20% of humans are seropositive for L. infantum. However, the sand fly species involved in L. infantum transmission cycle are still unknown. Between 2007 and 2010, 3654 sand flies were collected from different environments (indoor, peridomestic, farming and sylvatic areas to identify the main L. infantum vector(s. Nine sand fly species were identified. The Phlebotomus genus (n = 54 specimens; Phlebotomus (Ph duboscqi and Phlebotomus (Ph. rodhaini was markedly under-represented in comparison to the Sergentomyia genus (n = 3600 specimens; Sergentomyia (Se adleri, Se. clydei, Se. antennata, Se. buxtoni, Se. dubia, Se. schwetzi and Se. magna. Se. dubia and Se. schwetzi were the dominant species indoor and in peridomestic environments, near humans and dogs. Blood-meal analysis indicated their anthropophilic behavior. Some Se. schwetzi specimens fed also on dogs. The dissection of females in the field allowed isolating L. infantum from sand flies of the Sergentomyia genus (0.4% of Se. dubia and 0.79% of Se. schwetzi females. It is worth noting that one Se. dubia female not engorged and not gravid revealed highly motile metacyclic of L. infantum in the anterior part of the midgut. PCR-based diagnosis and sequencing targeting Leishmania kinetoplast DNA (kDNA highlighted a high rate of L. infantum-positive females (5.38% of Se. dubia, 4.19% of Se. schwetzi and 3.64% of Se. magna. More than 2% of these positive females were unfed, suggesting the parasite survival after blood-meal digestion or egg laying. L. infantum prevalence in Se. schwetzi was associated with its seroprevalence in dogs and humans and L. infantum prevalence in Se. dubia was associated with its seroprevalence in humans. These evidences altogether strongly suggest that

Efeito da aplicação de pó de basalto no desenvolvimento inicial de mudas de camu-camu (Myrciaria dubia

Directory of Open Access Journals (Sweden)

Marina Keiko Welter

2011-09-01

Full Text Available A produção de mudas de fruteiras nativas é um importante passo para a preservação das espécies, exploração comercial sustentável e geração de emprego. Neste contexto, os fertilizantes alternativos, como pó de rocha, propiciam a obtenção de um substrato com maior fertilidade, rico em nutrientes, com redução de custos em mão de obra e com a reposição de adubação. O objetivo no presente trabalho foi avaliar o desenvolvimento inicial de mudas de camu-camu (Myrciaria dubia H.B.K. McVaugh em função de doses de pó de basalto. O experimento foi conduzido em casa de vegetação pertencente ao CCA/UFRR. O delineamento experimental foi o de blocos ao acaso, com cinco repetições, em esquema fatorial (5x2+1, sendo seis doses de pó de basalto (0; 0,42;1,04; 2,08; 4,17 e 8,33 g kg-1, duas granulometrias (0,05 e 0,10 mm f e uma testemunha. Vasos de polietileno de 14 litros foram as unidades experimentais e utilizou-se um Latossolo Amarelo distrófico textura média. A incubação do solo nos vasos teve duração de 120 dias e, após esse período, fez-se o transplante das plântulas. Após seis meses do plantio, foram determinados altura, diâmetro do colo, número de ramos e biomassa seca da parte aérea e de raiz das mudas. A partir destes dados, foram calculados os índices morfológicos. Os tratamentos sem pó de basalto (0 g kg-1 e a dose de 0,42 g kg-1 produziram mudas de menor qualidade em ambas as granulometrias testadas. As melhores mudas de camu-camu foram obtidas aplicando-se 4,17 e 8,33 g kg-1 de pó de basalto com a granulometria de 0,05 mm.

Capacidad de enraizamiento de plantas matrices promisorias de Myrciaria dubia (Kunth Mc Vaugh en cámaras de subirrigación¹

Directory of Open Access Journals (Sweden)

Carlos Abanto Rodríguez

2014-02-01

Full Text Available Camu camu es una fruta nativa de la Amazonía, que llama la atención por el alto contenido de vitamina C (6,116 mg/ 100 g de pulpa, está en proceso de domesticación, por lo cual se está investigando un método de propagación vegetativa que permita avanzar en el proceso de mejoramiento genético. El objetivo de este trabajo fue evaluar la capacidad rizogénica de plantas matrices promisorias de camu camu "Myrciaria dubia (Kunth McVaugh" según el aumento del número de hojas, mediante la técnica de estacas herbáceas en cámaras de subirrigación. El ensayo fue conducido mediante un Diseño de Bloques Completamente al Azar (DBCA con arreglo factorial 9Ax3B, con 3 repeticiones y 15 estacas por unidad experimental. El factor A, estuvo constituido por nueve plantas matrices y el factor B: pares de hojas con 3 niveles: 1; 2 y 3 pares. El enraizamiento fue evaluado después de 90 días. Se observó que existió interacción estadística significativa para las variables: porcentaje de enraizamiento, longitud y número de raíces. Para las variables porcentaje de callo y porcentaje de mortalidad se encontró efecto de la planta matriz y pares de hojas. Los resultados muestran que el porcentaje de enraizamiento estuvo influenciado por efectos intrínsecos adherentes a la variabilidad genotípica de las plantas matrices, presentando un alto grado de dispersión, que osciló entre 91,11 % y 0,00 %, mostrando una alta variabilidad y marcada influencia de la planta matriz sobre el proceso de rizogénesis, influyendo de manera altamente significativa en el enraizamiento. Con respecto al área foliar, estacas con 2 y 3 pares de hojas, independiente de la planta matriz, presentaron mayor capacidad de enraizamiento. Con base en estos resultados se concluye que el efecto de la variabilidad genotípica y el área foliar influyen de manera altamente significativa en el proceso de rizogénesis de estacas herbáceas de camu camu.

Clonagem do camu-camu arbustivo em porta-enxertos de camu-camu arbustivo e arbóreo Cloning of shrubby camu-camu on shrubby and arboreal camu-camu rootstocks

Directory of Open Access Journals (Sweden)

Mario Moreira Filho

2009-12-01

Full Text Available O camu-camu (Myrciaria dubia (H.B.K. McVaugh, espécie frutífera nativa da Amazônia, de porte arbustivo, é propagado, normalmente, por sementes, ocasionando grande variação na entrada em frutificação e no ciclo de produção, bem como no teor de ácido ascórbico (vitamina C dos frutos. outra espécie de camu-camu, M. floribunda (West ex Willd. o. Berg, de porte arbóreo, ocorre em menor densidade na região amazônica. O objetivo deste trabalho foi avaliar a eficiência de diferentes métodos de enxertia e a compatibilidade interespecífica entre camu-camu arbustivo e camu-camu arbóreo na fase de formação de mudas de M. dubia. o ensaio foi desenvolvido no período de março a agosto de 2008, no Instituto Nacional de Pesquisas da Amazônia, em Manaus-AM, com delineamento experimental de blocos ao acaso, em esquema fatorial 4 (métodos de enxertia x 2 (espécies de porta-enxerto, com quatro repetições. M. dubia, como porta-enxerto, apresentou melhor percentual de pegamento de enxertos (78,4%, comparada a M. floribunda (49,3%. os melhores resultados na clonagem de M. dubia foram obtidos pelos métodos de garfagem, em que a parte aérea do porta-enxerto foi removida após 30 dias da enxertia: fenda lateral (89,3% e lateral com lingueta (79,3%. o menor resultado foi obtido nos métodos de garfagem, em que a parte aérea do porta-enxerto foi removida na ocasião da enxertia: topo em fenda cheia (51,6% e inglês complicado (31,5%.The camu-camu (Myrciaria dubia (H.B.K. McVaugh is a shrubby indigenous fruit species found in the floodplains of Amazonia region. There is considerable local and international commercial interest in this fruit, because of its high Vitamin C content. The species is commonly propagated by seeds, showing considerable variation in age of bearing, production cycle, fruit yields and Vitamin C contents. Another species, Myrciaria floribunda (West ex Willd. o. Berg, occurs less frequently in the region. The present study

Cover gas seals. 11 - FFTF-LMFBR seal-test program, January-March 1974

International Nuclear Information System (INIS)

Kurzeka, W.; Oliva, R.; Welch, F.

1974-01-01

The objectives of this program are to: (1) conduct static and dynamic tests to demonstrate or determine the mechanical performance of full-size (cross section) FFTF fuel transfer machine and reactor vessel head seals intended for use in a sodium vapor - inert gas environment, (2) demonstrate that these FFTF seals or new seal configuration provide acceptable fission product and cover gas retention capabilities at LMFBR Clinch River Plant operating environmental conditions other than radiation, and (3) develop improved seals and seal technology for the LMFBR Clinch River Plant to support the national objective to reduce all atmospheric contaminations to low levels

Intracellular conversion of environmental nitrate and nitrite to nitric oxide with resulting developmental toxicity to the crustacean Daphnia magna.

Directory of Open Access Journals (Sweden)

Bethany R Hannas

2010-08-01

Full Text Available Nitrate and nitrite (jointly referred to herein as NO(x are ubiquitous environmental contaminants to which aquatic organisms are at particularly high risk of exposure. We tested the hypothesis that NO(x undergo intracellular conversion to the potent signaling molecule nitric oxide resulting in the disruption of endocrine-regulated processes.These experiments were performed with insect cells (Drosophila S2 and whole organisms Daphnia magna. We first evaluated the ability of cells to convert nitrate (NO(3(- and nitrite (NO(2(- to nitric oxide using amperometric real-time nitric oxide detection. Both NO(3(- and NO(2(- were converted to nitric oxide in a substrate concentration-dependent manner. Further, nitric oxide trapping and fluorescent visualization studies revealed that perinatal daphnids readily convert NO(2(- to nitric oxide. Next, daphnids were continuously exposed to concentrations of the nitric oxide-donor sodium nitroprusside (positive control and to concentrations of NO(3(- and NO(2(-. All three compounds interfered with normal embryo development and reduced daphnid fecundity. Developmental abnormalities were characteristic of those elicited by compounds that interfere with ecdysteroid signaling. However, no compelling evidence was generated to indicate that nitric oxide reduced ecdysteroid titers.Results demonstrate that nitrite elicits developmental and reproductive toxicity at environmentally relevant concentrations due likely to its intracellular conversion to nitric oxide.

Interactive effects of genotype and food quality on consumer growth rate and elemental content.

Science.gov (United States)

Prater, Clay; Wagner, Nicole D; Frost, Paul C

2017-05-01

Consumer body stoichiometry is a key trait that links organismal physiology to population and ecosystem-level dynamics. However, as elemental composition has traditionally been considered to be constrained within a species, the ecological and evolutionary factors shaping consumer elemental composition have not been clearly resolved. To this end, we examined the causes and extent of variation in the body phosphorus (P) content and the expression of P-linked traits, mass specific growth rate (MSGR), and P use efficiency (PUE) of the keystone aquatic consumer Daphnia using lake surveys and common garden experiments. While daphnid body %P was relatively constrained in field assemblages sampled across an environmental P gradient, unique genotypes isolated from these lakes showed highly variable phenotypic responses when raised across dietary P gradients in the laboratory. Specifically, we observed substantial inter- and intra-specific variation and differences in daphnid responses within and among our study lakes. While variation in Daphnia body %P was mostly due to plastic phenotypic changes, we documented considerable genetic differences in daphnid MSGR and PUE, and relationships between MSGR and body P content were highly variable among genotypes. Overall, our study found that consumer responses to food quality may differ considerably among genotypes and that relationships between organismal life-history traits and body stoichiometry may be strongly influenced by genetic and environmental variation in natural assemblages. © 2017 by the Ecological Society of America.

Acute sensitivity of three Cladoceran species to different types of microplastics in combination with thermal stress.

Science.gov (United States)

Jaikumar, Gayathri; Baas, Jan; Brun, Nadja R; Vijver, Martina G; Bosker, Thijs

2018-08-01

Microplastics (microplastics on freshwater ecosystems, especially under different environmental conditions. In the present study, the sensitivity of two temperate Cladoceran species, Daphnia magna and Daphnia pulex, and a smaller tropical species Ceriodaphnia dubia, to primary microplastics (PMP) and secondary (weathered) microplastics (SMP) was assessed. A prolonged acute toxicity assay (up to 72 or 96 h) was performed at 18°, 22°, and 26 °C, to determine the influence of temperature as an additional stressor and survival data were analysed using toxicokinetic-toxicodynamic (TK-TD) model. Acute sensitivity of D. magna and D. pulex to both PMP and SMP increased sharply with temperature, whereas that of C. dubia remained relatively stable across temperatures. C. dubia was the most sensitive species at 18 °C, followed by D. pulex and D. magna, which were of comparable sensitivity. However, this ranking was reversed at 26 °C as could be seen from the No Effect Concentration (NEC) estimates of the TK-TD model. In addition, SMP and PMP had a similar effect on D. magna and D. pulex, but PMP was more toxic to C. dubia. Effects on survival were strongly time-dependent and became substantially more severe after the standard 48 h test period. Our results indicate that sensitivity to microplastics may differ between species for different types of microplastics, and could be drastically influenced by temperature albeit at high exposure concentrations. Copyright © 2018 Elsevier Ltd. All rights reserved.

Biological control of toxic cyanobacteri

CSIR Research Space (South Africa)

Ndlela, Luyanda L

2017-05-01

Full Text Available . Ecotoxicity studies: is toxicity reduced? (Testing on daphnids, fish and human cell lines) Resulting impacts on cyanotoxins (Toxin conformation changes, ELISA detection) Competition assays against toxic cyanobacteria (Can Bacillus etc. outcompete...

Quantum chemistry based quantitative structure-activity relationships for modeling the (sub)acute toxicity of substituted mononitrobenzenes in aquatic systems

NARCIS (Netherlands)

Zvinavashe, E.; Murk, A.J.; Vervoort, J.; Soffers, A.E.M.F.; Freidig, A.; Rietjens, I.M.C.M.

2006-01-01

Fifteen experimental literature data sets on the acute toxicity of substituted nitrobenzenes to algae (Scenedesmus obliquus, Chlorella pyrenoidosa, C. vulgaris), daphnids (Daphnia magna, D. carinata), fish (Cyprinus carpio, Poecilia reticulata), protozoa (Tetrahymena pyriformis), bacteria

LMFBR fuel analysis. Task A: oxide fuel dynamics. Final report, July 1, 1975--September 30, 1976

International Nuclear Information System (INIS)

Dhir, V.K.; Hauss, B.; Kastenberg, W.E.; Saqui, R.; Sun, Y.H.; Wong, K.

1976-11-01

The report summarizes the results of studies conducted in support of the U.S. Nuclear Regulatory Commission's review of the Preliminary Safety Analysis Report for the Clinch River Breeder Reactor. In particular it deals with three aspects of the unprotected transient overpower accident. The first aspect is the response of the Clinch River Breeder Reactor to low reactivity insertion rates. Second, the investigation of a new method for computing the time, place and mode of fuel pin failure is studied. Lastly, the question of post-failure, fuel freezing, and plate-out is addressed. Several areas of uncertainty in the analysis of these accidents is also discussed

Analysis of the TREAT loss-of-flow tests L6 and L7 using SAS3D

International Nuclear Information System (INIS)

Morris, E.E.; Simms, R.; Gruber, E.E.

1985-01-01

The TREAT loss-of-flow tests L6 and L7 have been analyzed using the SAS3D accident analysis code. The impetus for the analysis was the need for experimentally supported fuel motion modeling in whole core accident studies performed in support of licensing of the Clinch River Breeder Reactor Project. The input prescription chosen for the SAS3D/SLUMPY fuel motion model gave reasonable agreement with the test results. Tests L6 and L7, each conducted with a cluster of three fuel pins, were planned to simulate key events in the loss-of-flow accident scenario for the Clinch River homogeneous reactor

Hydrodynamic trails produced by Daphnia: size and energetics.

Science.gov (United States)

Wickramarathna, Lalith N; Noss, Christian; Lorke, Andreas

2014-01-01

This study focuses on quantifying hydrodynamic trails produced by freely swimming zooplankton. We combined volumetric tracking of swimming trajectories with planar observations of the flow field induced by Daphnia of different size and swimming in different patterns. Spatial extension of the planar flow field along the trajectories was used to interrogate the dimensions (length and volume) and energetics (dissipation rate of kinetic energy and total dissipated power) of the trails. Our findings demonstrate that neither swimming pattern nor size of the organisms affect the trail width or the dissipation rate. However, we found that the trail volume increases with increasing organism size and swimming velocity, more precisely the trail volume is proportional to the third power of Reynolds number. This increase furthermore results in significantly enhanced total dissipated power at higher Reynolds number. The biggest trail volume observed corresponds to about 500 times the body volume of the largest daphnids. Trail-averaged viscous dissipation rate of the swimming daphnids vary in the range of 1.8 x 10(-6) W/kg to 3.4 x 10(-6) W/kg and the observed magnitudes of total dissipated power between 1.3 x 10(-9) W and 1 x 10(-8) W, respectively. Among other zooplankton species, daphnids display the highest total dissipated power in their trails. These findings are discussed in the context of fluid mixing and transport by organisms swimming at intermediate Reynolds numbers.

Surface metal adsorption on zooplankton carapaces: implications for exposure and effects in consumer organisms

International Nuclear Information System (INIS)

Robinson, K.A.; Baird, D.J.; Wrona, F.J.

2003-01-01

Metals adsorbed to prey surfaces may be a mechanism of exposure in predators. - The current study aimed to determine the potential of two important aquatic invertebrate crustacean species, Daphnia magna and Ceriodaphnia dubia, to adsorb cadmium on to their carapaces from aqueous solution. Using the Langmuir equation to model data outputs, it was shown that cadmium readily became associated with the carapace surfaces of both species, with uptake being dependent on exposure time and concentration. Maximum carapace-adsorption potential was found to be directly related to surface area, so that at predicted carapace saturation, D. magna neonates bound approximately five times more cadmium than the smaller C. dubia neonates. However, adsorption per unit surface area was found to be similar under the same exposure conditions. Results of surface metal adsorption studies in C. dubia suggested that short term exposures to high concentrations of aqueous cadmium would lead to similar levels of adsorption as obtained with long-term exposures to low concentrations. The study illustrates that contaminants adsorbed to prey surfaces may be an important mechanism of exposure to predators, and highlights some potential problems of feeding organisms during long-term toxicity tests

Remedial investigation/feasibility study report for lower Watts Bar Reservoir Operable Unit

International Nuclear Information System (INIS)

1994-08-01

This document is the combined Remedial Investigation and Feasibility Study Report for the Lower Watts Bar Reservoir (LWBR) Operable Unit (OU). The LWBR is located in Roane, Rhea, and Meigs counties, Tennessee, and consists of Watts Bar Reservoir downstream of the Clinch River. This area has received hazardous substances released over a period of 50 years from the U.S. Department of Energy's Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA). As required by this law, the ORR and all off-site areas that have received containments, including LWBR, must be investigated to determine the risk to human health and the environment resulting from these releases, the need for any remedial action to reduce these risks, and the remedial actions that are most feasible for implementation in this OU. Contaminants from the ORR are primarily transported to the LWBR via the Clinch River. Water-soluble contaminants released to ORR surface waters are rapidly diluted upon entering the Clinch River and then quickly transported downstream to the Tennessee River where further dilution occurs. Almost the entire quantity of these diluted contaminants rapidly flows through LWBR. In contrast, particle-associated contaminants tend to accumulate in the lower Clinch River and in LWBR in areas of sediment deposition. Those particle-associated contaminants that were released in peak quantities during the early years of ORR operations (e.g., mercury and 137 Cs) are buried under as much as 80 cm of cleaner sediment in LWBR. Certain contaminants, most notably polychlorinated biphenyls (PCBs), have accumulated in LWBR biota. The contamination of aquatic biota with PCBs is best documented for certain fish species and extends to reservoirs upstream of the ORR, indicating a contamination problem that is regional in scope and not specific to the ORR

Initiation of the midsummer decline of Daphnia as related to predation, non-consumptive mortality and recruitment: a balance

NARCIS (Netherlands)

Wagner, A.; Hülsmann, S.; Dörner, H.; Janssen, M.; Kahl, U.; Mehner, T.; Benndorf, J.

2004-01-01

The midsummer decline (MSD) of daphnids has been attributed to a range of factors related to either resource availability or predation. However, the relative magnitude and importance of each of these factors remained unknown. Therefore, we quantified simultaneously, but independently, both

Water quality monitoring report for the White Oak Creek Embayment

International Nuclear Information System (INIS)

Ford, C.J.; Wefer, M.T.

1993-01-01

Water quality monitoring activities that focused on the detection of resuspended sediments in the Clinch River were conducted in conjunction with the White Oak Creek Embayment (WOCE) time-critical Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) removal action to construct a sediment-retention structure at the mouth of White Oak Creek (WOC). Samples were collected by use of a 24-h composite sampler and through real-time water grab sampling of sediment plumes generated by the construction activities. Sampling stations were established both at the WOC mouth, immediately adjacent to the construction site, and at K-1513, the Oak Ridge K-25 Site drinking water intake approximately 9.6 km downstream in the Clinch River. Results are described

Preliminary screening analysis of the off-site environment downstream of the US Department of Energy Oak Ridge Reservation

International Nuclear Information System (INIS)

Blaylock, B.G.; Hoffman, F.O.; Frank, M.L.

1990-01-01

Operations and waste disposal activities at the Y-12 Plant, the Oak Ridge National Laboratory (ORNL), and the Oak Ridge Gaseous Diffusion Plant (ORGDP), located on the US Department of Energy (DOE) Oak Ridge Reservation (ORR) in eastern Tennessee, have introduced airborne, liquid, and solid wastes into the surrounding environment. Some of these wastes may affect off-site areas by entering local streams that ultimately drain into the Clinch River. Previously reported concentrations of radionuclides, metals, and organic compounds in water, sediment, and biota of the Clinch River and Watts Bar Reservoir suggest the presence of contaminants of possible concern to the protection of human health and the environment. A preliminary screening was conducted of contaminants in the off-site surface water environments downstream of the DOE ORR. This screening analysis represents part of a scoping phase of the Clinch River Resource Conservation and Recovery Facilities Investigation (CRRFI). The purpose of this preliminary screening analysis is to use existing data on off-site contaminant concentrations to identify and prioritize potential contaminants of concern for further evaluation and investigation. The primary objective of this screening analysis is to ensure that CRRFI sampling and analysis efforts focus on those contaminants that may possibly contribute to human health or environmental risk. 8 refs., 3 figs., 6 tabs

Posibles factores que producen la caída de fruto de Myrciaria dubia (HBK Mc Vaugh, "camu camu" durante la fenología reproductiva de la colección "cinco cuencas" en el centro experimental San Miguel - IIAP, Loreto, Perú

Directory of Open Access Journals (Sweden)

Sonia Farro

2010-01-01

Full Text Available Se evaluaron plantas de “camu camu” en cinco cuencas de Loreto (Perú, según el porcentaje de frutos con síntomas de infestación por plagas, la retención de flores y frutos en cada una de las cuencas y diámetros de ramas, y la influencia de la precipitación y temperatura en el proceso de caída de frutos según el estado fenológico. En el factor genético, la cuenca del río Putumayo destacó por presentar mayor retención de frutos, mayor rendimiento y peso promedio de frutos, así como menor ataque por plagas. Durante el proceso fenológico que duró 12 semanas, la etapa crítica de caída de flores y frutos ocurrió durante las primeras 7, siendo la retención de flores del 5.12%. Las plagas observadas son causantes del 9.27% de la caída, siendo el 9.15% causada por Edessa sp., y 0.12% por Conotrachellus dubiae. El otro 90.73% fue originado por otros factores no determinados tales como fisiológicos, nutritivos, competencia, vientos, lluvia. Los factores ambientales de temperatura y precipitación, ejercen una influencia directa e inversamente proporcional a la caída de frutos, respectivamente.

Evaluation of lithium as a toxicant and the modifying effect of sodium

International Nuclear Information System (INIS)

Kszos, L.A.; Stewart, A.J.; O'Reilly, S.E.

1995-01-01

Routine compliance tests conducted for a groundwater treatment facility at the Y-12 Plant on the Department of Energy's (DOE) Oak Ridge Reservation (ORR), TN, showed that the effluent was acutely toxic to Ceriodaphnia dubia and fathead minnow (Pimephales promelas) larvae. An evaluation of suspected contaminants revealed that increased toxicity coincided with increased concentrations of lithium. Lithium is a light, strong metal that is used in DOE operations, including fusion weapons and fission reactors. Little has been published about lithium toxicity. Toxicity tests were conducted with fathead minnows and C. dubia using lithium chloride and lithium tetraborate. Dilute mineral water (DMW) or the receiving stream water (East Fork Poplar Creek) was used as the dilution water in the toxicity evaluation. A concentration of 1 mg Li/L in DMW reduced the survival of both test species; 0.5 mg Li/L in DMW reduced C. dubia reproduction and minnow growth. Sodium appears to influence the toxicity of Li; the metal was six times more toxic in the low-sodium DMW than in stream water containing 30 mg Na/L. Tests with LiCl in combination with NaCl and NA 2 SO 4 demonstrated that the presence of sodium reduced the toxicity of Li to C. dubia. In laboratory tests with a snail (Elimia clavaeformis) common on the ORR, the feeding rate declined in 0.15 mg Li/L. Because Li has also been demonstrated to be toxic to several plant species, tests with LiCi were also conducted using buttercrunch lettuce (Lactuca saliva). The EC 50 for seed growth after 8 d incubation was 37.5 mg Li/L. These findings are significant because of widespread industrial use and potential accumulation of Li in soils

Lindernia dubia (L.) Pennell

Czech Academy of Sciences Publication Activity Database

Šumberová, Kateřina; Juříček, M.; Paulič, R.

2016-01-01

Roč. 51, č. 1 (2016), s. 104-107 ISSN 1211-5258 R&D Projects: GA ČR GB14-36079G Institutional support: RVO:67985939 Keywords : plant propagule dispersal * wetlands * plant invasions Subject RIV: EF - Botanics

Monthly report No. 9 (September, October, and November 1977) of INTERATOM representative to Westinghouse Hanford Co., Richland, Washington (USA)

Energy Technology Data Exchange (ETDEWEB)

Mayer, H.

1977-11-30

The following topics are mentioned: alternate breeder fuel cycles (Shippingport Light Water Breeder Reactor), Clinch River Breeder Reactor status, FFTF status, LMFBR component development, and PLBR status. (DG)

24 CFR 3280.504 - Condensation control and installation of vapor retarders.

Science.gov (United States)

2010-04-01

..., Brooks, Bryan, Calhoun, Camden, Charlton, Chatham, Clay, Clinch, Coffee, Colquitt, Cook, Crisp, Decatur..., Thomas, Tift, Turner, Ware, Worth. Hawaii All counties and locations within the State of Hawaii...

Needs for reactivity anomaly monitoring in CRBRP

International Nuclear Information System (INIS)

Bullock, J.B.

1975-01-01

Two general classifications of reactivity anomalies are defined and explicit design criteria and operational philosophy for an anomaly monitoring system for the Clinch River Breeder Reactor are presented. (JWR)

To flee or not to flee: detection, avoidance and attraction of profitable resources by Daphnia magna studied with olfactometer

Directory of Open Access Journals (Sweden)

Johann P. Müller

2013-10-01

Full Text Available The cladoceran herbivore Daphnia magna is a major consumer of phytoplankton in lakes. Therefore, this organism may control the phytoplankton community and the proliferation of some algae or cyanobacteria. Cladoceran behaviour and migration in relation to temperature, light or presence of planktivorous fishes have been well studied. In particular, it is known that the detection of kairomones produced by predators may induce avoidance. Avoidance could also occur with other semiochemicals such as cyanotoxins. In order to explore this hypothesis, we used an olfactometer to observe and measure the exploratory behaviour of D. magna individuals based on the motivation for food. Daphnids were allowed to choose between different compounds: water, a pure cyanotoxin, i.e. the microcystin-RR [(MC-RR], extracts of one MC-producing strain (PMC 75.02 and one MC-free strain (PMC 87.02 of Planktothrix agardhii, or a green algae Scenedesmus obliquus. With this experimental design, we observed that i cladocerans are able to detect resources with different qualities, ii they can explore before exhibiting preferences, and iii daphnids are able to avoid compounds that are potentially toxic (e.g., microcystins. First, daphnids explored the environment, subsequently (after about 1.5 h, they showed a significant tendency to stay where there is a profitable resource such as S. obliquus. These results also suggest that specimens of D. magna cannot detect MC compounds from P. agardhii, but they respond to it as a food resource. The study of zooplankton ability to explore the environment when exposed to semiochemicals needs further investigation. 

Pollination of Cambessedesia wurdackii in Brazilian campo rupestre vegetation, with special reference to crepuscular bees.

Science.gov (United States)

Franco, Emanuella Lopes; Gimenes, Miriam

2011-01-01

Cambessedesia wurdackii Martins (Myrtales: Melastomataceae) is presumably endemic to the Chapada Diamantina, Bahia State, Brazil. A majority of the species of this family are pollinated by diurnal bees that buzz the floral anthers to collect pollen. The present work examined the interactions between C. wurdackii and visiting bees, focusing on temporal, morphological, and behavioral features, especially in regards to the crepuscular bees Megalopta sodalis (Vachal) (Hymenoptera: Halictidae) and Ptiloglossa off. dubia Moure (Hymenoptera: Colletidae). The study was undertaken in an area of campo rupestre montane savanna vegetation located in the Chapada Diamantina Mountains of Bahia State, Brazil, between August/2007 and July/2008. Flowering in C. wurdackii occurred from April through July, with a peak in May. A total of 592 visits by diurnal and crepuscular bees to the flowers of C. wurdackii were recorded, with a majority of the visits made by M. sodalis and P. dubia (92%) near sunrise and sunset. The anthers of C. wurdackii are arranged in two tiers, which favors cross pollination. The morphological, temporal and behavioral characteristics of M. sodalis and P. dubia indicated that they were potential pollinators of C. wurdackii, in spite of the fact that the colorful and showy flowers of this species are more typical of a diurnal melittophilous pollination syndrome.

The significance of nonviable eggs for Daphnia population dynamics

NARCIS (Netherlands)

Boersma, M.; Vijverberg, J.

1995-01-01

Egg mortality was studied in populations of Daphnia galeata, Daphnia cucullata, and the hybrid between these species. In Tjeukemeer, a shallow eutrophic lake in the Netherlands, egg mortality in daphnids manifested itself as an apparent increase in the frequency of eggs in the early developmental

Toxicity evaluation of natural samples from the vicinity of rice fields using two trophic levels.

Science.gov (United States)

Marques, Catarina R; Pereira, Ruth; Gonçalves, Fernando

2011-09-01

An ecotoxicological screening of environmental samples collected in the vicinity of rice fields followed a combination of physical and chemical measurements and chronic bioassays with two freshwater trophic levels (microalgae: Pseudokirchneriella subcapitata and Chlorella vulgaris; daphnids: Daphnia longispina and Daphnia magna). As so, water and sediment/soil elutriate samples were obtained from three sites: (1) in a canal reach crossing a protected wetland upstream, (2) in a canal reach surrounded by rice fields and (3) in a rice paddy. The sampling was performed before and during the rice culture. During the rice cropping, the whole system quality decreased comparatively to the situation before that period (e.g. nutrient overload, the presence of pesticides in elutriates from sites L2 and L3). This was reinforced by a significant inhibition of both microalgae growth, especially under elutriates. Contrary, the life-history traits of daphnids were significantly stimulated with increasing concentrations of water and elutriates, for both sampling periods.

Development and application of a sediment toxicity test using the benthic cladoceran Chydorus sphaericus

International Nuclear Information System (INIS)

Dekker, T.; Greve, G.D.; Ter Laak, T.L.; Boivin, M.E.; Veuger, B.; Gortzak, G.; Dumfries, S.; Luecker, S.M.G.; Kraak, M.H.S.; Admiraal, W.; Geest, H.G. van der

2006-01-01

This study reports on the development and application of a whole sediment toxicity test using a benthic cladoceran Chydorus sphaericus, as an alternative for the use of pelagic daphnids. A C. sphaericus laboratory culture was started and its performance under control conditions was optimised. The test was firstly validated by determining dose-response relationships for aqueous cadmium and copper and ammonia, showing a sensitivity of C. sphaericus (96 h LC 5 values of 594 μg Cd/L, 191 μg Cu/L and 46 mg ammonia/L at pH 8) similar to that of daphnids. Next, sediment was introduced into the test system and a series of contaminated sediments from polluted locations were tested. A significant negative correlation between survival and toxicant concentrations was observed. It is concluded that the test developed in the present study using the benthic cladoceran C. sphaericus is suitable for routine laboratory sediment toxicity testing. - A test was developed for assaying sediment toxicity using a commonly occurring small-bodied cladoceran

Estimated radiological doses to the maximumly exposed individual and downstream populations from releases of tritium, strontium-90, ruthenium-106, and cesium-137 from White Oak Dam

International Nuclear Information System (INIS)

Little, C.A.; Cotter, S.J.

1980-01-01

Concentrations of tritium, 90 Sr, 106 Ru, and 137 Cs in the Clinch River for 1978 were estimated by using the known 1978 releases of these nuclides from the White Oak Dam and diluting them by the integrated annual flow rate of the Clinch River. Estimates of 50-year dose commitment to a maximumly exposed individual were calculated for both aquatic and terestrial pathways of exposure. The maximumly exposed individual was assumed to reside at the mouth of White Oak Creek where it enters the Clinch River and obtain all foodstuffs and drinking water at that location. The estimated total-body dose from all pathways to the maximumly exposed individual as a result of 1978 releases was less than 1% of the dose expected from natural background. Using appropriate concentrations of to subject radionuclides diluted downstream, the doses to populations residing at Harriman, Kingston, Rockwood, Spring City, Soddy-Daisy, and Chattanooga were calculated for aquatic exposure pathways. The total-body dose estimated for aquatic pathways for the six cities was about 0.0002 times the expected dose from natural background. For the pathways considered in this report, the nuclide which contributed the largest fraction of dose was 90 Sr. The largest dose delivered by 90 Sr was to the bone of the subject individual or community

Phototransformation of Amlodipine in Aqueous Solution: Toxicity of the Drug and Its Photoproduct on Aquatic Organisms

Directory of Open Access Journals (Sweden)

Marina DellaGreca

2007-01-01

Full Text Available The phototransformation of amlodipine in water was investigated under various conditions. A quantum yield ΦS2.2×10−4 and a half-life time t1/2 0.419 days were calculated when the drug in water (10−4 M was exposed to sunlight. The only photoproduct found was its pyridine derivative. Formation of this product was explained on the basis of a radical cation intermediate. The acute and chronic toxicity of the drug and its photoproduct were evaluated on different organisms of the freshwater chain (Brachionus calyciflorus, Thamnocephalus platyurus, Daphnia magna, Ceriodaphnia dubia. The photoproduct exhibited a stronger toxic potential than the parent drug on the long time for C. dubia.

Integral Monitored Retrievable Storage (MRS) Facility conceptual design report

International Nuclear Information System (INIS)

1985-09-01

This document, Volume 5 Book 7, contains cost estimate information for a monitored retrievable storage (MRS) facility. Cost estimates are for onsite improvements, waste storage, and offsite improvements for the Clinch River Site

Strategies for Transferring Mixtures of Organic Contaminants from Aquatic Environments into Bioassays

DEFF Research Database (Denmark)

Jahnke, Annika; Mayer, Philipp; Schäfer, Sabine

2016-01-01

and monitoring of such mixtures, a variety of cell-based in vitro and low-complexity in vivo bioassays based on algae, daphnids or fish embryos are available. A very important and sometimes unrecognized challenge is how to combine sampling, extraction and dosing to transfer the mixtures from the environment...

MINET: transient analysis of fluid-flow and heat-transfer networks

International Nuclear Information System (INIS)

Van Tuyle, G.J.; Guppy, J.G.; Nepsee, T.C.

1983-01-01

MINET, a computer code developed for the steady-state and transient analysis of fluid-flow and heat-transfer networks, is described. The code is based on a momentum integral network method, which offers significant computational advantages in the analysis of large systems, such as the balance of plant in a power-generating facility. An application is discussed in which MINET is coupled to the Super System Code (SSC), an advanced generic code for the transient analysis of loop- or pool-type LMFBR systems. In this application, the ability of the Clinch River Breeder Reactor Plant to operate in a natural circulation mode following an assumed loss of all electric power, was assessed. Results from the MINET portion of the calculations are compared against those generated independently by the Clinch River Project, using the DEMO code

Identification of causes of oil sands coke leachate toxicity

International Nuclear Information System (INIS)

Puttaswamy, N.; Liber, K.

2010-01-01

The potential causes of oil sands coke leachate toxicity were investigated. Chronic 7-day toxicity tests were conducted to demonstrate that oil sands coke leachates (CL) are acutely toxic to Ceriodaphnia dubia (C. dubia). CLs were generated in a laboratory to perform toxicity identification evaluation (TIE) tests in order to investigate the causes of the CL toxicity. The coke was subjected to a 15-day batch leaching process at 5.5 and 9.5 pH values. The leachates were then filtered and used for chemical and toxicological characterization. The 7-day estimates for the C. dubia survival were 6.3 for a pH of 5.5 and 28.7 per cent for the 9.5 CLs. The addition of EDTA significantly improved survival and reproduction in a pH of 5.5 CL, but not in a pH of 9.5 CL. The toxicity of the pH 5.5 CL was removed with a cationic resin treatment. The toxicity of the 9.5 pH LC was removed using an anion resin treatment. Toxicity re-appeared when nickel (Ni) and vanadium (V) were added back to the resin-treated CLs. Results of the study suggested that Ni and V were acting as primary toxicants in the pH 5.5 CL, while V was the primary cause of toxicity in the pH 9.5 CL.

Influência do tamanho da semente na germinação e vigor de plântulas de populações de Camu-Camu

Directory of Open Access Journals (Sweden)

Olisson Mesquita Souza

2017-01-01

Full Text Available Camu - camu ( Myr ciaria dubia is a fruit species native to the Amazon and its economic importance is proven because the fruit contains high content of ascorbic acid. The objective of this work was to evaluate the germination and vigor of seedlings obtained from seeds of d ifferent sizes of two camu - camu populations. The experimental design was completely randomized in a factorial scheme of 2 x 3 (two provenances river Anauá and river Urubu and three seed sizes, small, medium and large with 5 replicates of 20 seeds per plot . The seeds of the origin of the Anauá river presented higher mass (1.46 g compared to the origin of the Urubu river (0.78 g. The speed and emergence of seed germination presented better values for the population of the Anauá river, justifying the adopti on of choice of origin for the formation of seedlings. For root length, dry shoot mass and dry mass of the root system, the seeds classified as medium and large originated from the Anauá river presented higher values. The size of the seeds is an important character to be used in the production of Myrciaria dubia seedlings. The seeds of the origin of the Anauá river are potentially more vigorous are indicate for the production of camu - camu ( Myrciaria dubia seedlings.

Estrategias bioinformáticas y moleculares para aislar genes con potencial biotecnológico a partir de especies huérfanas de la ciencia

Directory of Open Access Journals (Sweden)

Juan Castro Gómez

2013-12-01

Full Text Available La amazonía peruana es megabiodiversa y posee millones de genes con potencial biotecnológico que debemos descubrir. Para identificarlos, aislarlos y poder aprovecharlos tenemos que secuenciar los genomas y transcriptomas de las especies más importantes. Sin embargo, hasta concretar este tipo de proyectos, combinando estrategias bioinformáticas y moleculares podemos aislar genes con potencial biotecnológico. El objetivo fue aislar genes involucrados en la biosíntesis de vitamina C en Myrciaria dubia. De acuerdo a las relaciones filogenéticas de M. dubia con otras especies, se buscaron secuencias homólogas en el banco de genes del gen que codifica L-galactono-1,4-lactona deshidrogenasa (L-GalLDH. En base al alineamiento múltiple obtenido, se construyó un filograma consenso y de los clados formados se diseñaron cebadores degenerados. Los frutos fueron obtenidos de una colección de germoplasma y el ARN total se purificó, el ADNc se sintetizó y amplificó con cebadores degenerados. El amplicón sintetizado se clonó y secuenció con técnicas estándares. Con las aproximaciones empleadas se logró aislar, clonar y secuenciar un segmento de 921 pb del gen L-GalLDH de M. dubia. En conclusión, con las estrategias bioinformáticas y moleculares descritas se aisló el segmento de un gen que codifica la enzima L-galactono-1,4-lactona deshidrogenasa de la vía biosintética de vitamina C en M. dubia, especie de la que no se disponía de secuencias génicas. Con estas mismas aproximaciones será posible aislar genes de interés biotecnológico de esta y otras especies amazónicas que aún no cuentan con bases de datos de secuencias nucleotídicas.

Remedial investigation/feasibility study report for Lower Watts Bar Reservoir Operable Unit

International Nuclear Information System (INIS)

1995-03-01

This document is the combined Remedial Investigation and Feasibility Study Report for the lower Watts Bar Reservoir (LWBR) Operable Unit (OU). The LWBR is located in Roane, Rhea, and Meigs counties, Tennessee, and consists of Watts Bar Reservoir downstream of the Clinch river. This area has received hazardous substances released over a period of 50 years from the US Department of Energy's Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA). As required by this law, the ORR and all off-site areas that have received contaminants, including LWBR, must be investigated to determine the risk to human health and the environment resulting from these releases, the need for any remedial action to reduce these risks, and the remedial actions that are most feasible for implementation in this OU. Contaminants from the ORR are primarily transported to the LWBR via the Clinch River. There is little data regarding the quantities of most contaminants potentially released from the ORR to the Clinch River, particularly for the early years of ORR operations. Estimates of the quantities released during this period are available for most radionuclides and some inorganic contaminants, indicating that releases 30 to 50 years ago were much higher than today. Since the early 1970s, the release of potential contaminants has been monitored for compliance with environmental law and reported in the annual environmental monitoring reports for the ORR

Remedial investigation/feasibility study report for Lower Watts Bar Reservoir Operable Unit

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-03-01

This document is the combined Remedial Investigation and Feasibility Study Report for the lower Watts Bar Reservoir (LWBR) Operable Unit (OU). The LWBR is located in Roane, Rhea, and Meigs counties, Tennessee, and consists of Watts Bar Reservoir downstream of the Clinch river. This area has received hazardous substances released over a period of 50 years from the US Department of Energy`s Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA). As required by this law, the ORR and all off-site areas that have received contaminants, including LWBR, must be investigated to determine the risk to human health and the environment resulting from these releases, the need for any remedial action to reduce these risks, and the remedial actions that are most feasible for implementation in this OU. Contaminants from the ORR are primarily transported to the LWBR via the Clinch River. There is little data regarding the quantities of most contaminants potentially released from the ORR to the Clinch River, particularly for the early years of ORR operations. Estimates of the quantities released during this period are available for most radionuclides and some inorganic contaminants, indicating that releases 30 to 50 years ago were much higher than today. Since the early 1970s, the release of potential contaminants has been monitored for compliance with environmental law and reported in the annual environmental monitoring reports for the ORR.

Comparison of Toxicity of Sediments from Rivers with Different Levels of Anthropogenic Load (Middle Volga Region, Russia Based on Elutriate and Whole Sediment Tests

Directory of Open Access Journals (Sweden)

N.Yu. Stepanova

2016-09-01

Full Text Available Description of chemical characteristics and toxicity testing of whole sediment and elutri-ate have been performed with 35 samples taken during the monitoring of rivers in the Middle Volga region (Tatarstan, Russia in 2013. The locations analyzed are sites associated with agriculture, forestry, and petroleum hydrocarbons (oil production. The toxicity tests include: (1 Chlorella vulgaris (algal elutriate test, (2 Paramecium caudatum (ciliate elutriate test, (3 Daphnia magna (cladoceran whole sediment toxicity test, and (4 Heterocypris incongruens (ostracod whole sediment toxicity test. The concentrations of metals in 43% of sediment samples have been found to exceed probable effect concentration sediment quality guidelines (SQGs. However, the concentrations of polycyclic aromatic hydrocarbons (PAHs and organochlorine pesticides have turned out to be below SQGs in most sites. The correlation analysis has shown metal toxicity to daphnid reproduction and ostracod growth (R2 = 0.34–0.64 and ammonia (R2 = 0.49–0.54. A higher percentage of samples have shown toxicity in the whole sediment tests (86% compared to the elutriate tests (54%. A total of 91% of samples have demonstrated toxicity for at least one species. Toxicity has been most frequently observed for daphnid reproduction (83% of samples and ostracod growth (56% of samples compared to daphnid (23% survival, ostracod (11% survival, and ciliate reproduction (54% or algal growth (54%. The most polluted sediments have been registered in the area of oil production. The comparison of toxicity of the samples from different types of areas has indicated that 100% of samples from the oil production area, 94% of samples from the agricultural area, and 50% of samples from the forest area were toxic to at least one test organism.

Influence of environmental factors on the response of a natural population of Daphnia magna (Crustacea: Cladocera) to spinosad and Bacillus thuringiensis israelensis in Mediterranean coastal wetlands

Energy Technology Data Exchange (ETDEWEB)

Duchet, C. [Entente Interdepartementale de Demoustication du Littoral Mediterraneen, 165 avenue Paul-Rimbaud, Montpellier F-34184 (France); INRA, UMR985 Ecologie et Sante des Ecosystemes, Equipe Ecotoxicologie et Qualite des Milieux Aquatiques, 65 rue de Saint Brieuc, Rennes F-35042 (France); Caquet, Th. [INRA, UMR985 Ecologie et Sante des Ecosystemes, Equipe Ecotoxicologie et Qualite des Milieux Aquatiques, 65 rue de Saint Brieuc, Rennes F-35042 (France); Franquet, E. [Universite Paul Cezanne, Institut Mediterraneen d' Ecologie et de Paleoecologie, Faculte des Sciences et Techniques Saint Jerome, C31, Marseille F-13397 (France); Lagneau, C. [Entente Interdepartementale de Demoustication du Littoral Mediterraneen, 165 avenue Paul-Rimbaud, Montpellier F-34184 (France); Lagadic, L., E-mail: Laurent.Lagadic@rennes.inra.f [INRA, UMR985 Ecologie et Sante des Ecosystemes, Equipe Ecotoxicologie et Qualite des Milieux Aquatiques, 65 rue de Saint Brieuc, Rennes F-35042 (France)

2010-05-15

The present study was undertaken to assess the impact of a candidate mosquito larvicide, spinosad (8, 17 and 33 mug L{sup -1}) on a field population of Daphnia magna under natural variations of water temperature and salinity, using Bti (0.16 and 0.50 muL L{sup -1}) as the reference larvicide. Microcosms (125 L) were placed in a shallow temporary marsh where D. magna was naturally present. The peak of salinity observed during the 21-day observation period may have been partly responsible for the decrease of daphnid population density in all the microcosms. It is also probably responsible for the absence of recovery in the microcosms treated with spinosad which caused a sharp decrease of D. magna abundance within the first two days following treatment whereas Bti had no effect. These results suggest that it may be difficult for a field population of daphnids to cope simultaneously with natural (water salinity and temperature) and anthropogenic (larvicides) stressors. - Significant interaction between salinity and spinosad exposure impairs the recovery of a natural population of Daphnia magna.

Multigenerational cadmium acclimation and biokinetics in Daphnia magna

International Nuclear Information System (INIS)

Guan Rui; Wang Wenxiong

2006-01-01

A Cd exposure (3 μg L -1 ) experiment was conducted for six successive generations to investigate the responses to chronic Cd stress in Daphnia magna. We observed a biphasic accumulation of Cd in the six generations and suggested a similar pattern with respect to daphnids' tolerance. Cd assimilation efficiencies, daphnid growth, and reproduction corresponded to the changes of tolerance, which was partially accounted for by metallothionein induction. When maternally exposed neonates grew in Cd-free water for one or two generations, their growth, MT concentration and biokinetic parameters partially or totally recovered. The rapid recovery suggests the high potential for ecological restoration from Cd pollution. Our results indicate that the tolerance of sensitive D. magna clones to Cd was dependent on long-term or multigenerational exposure. The tolerance developed within the first several generations might not be maintained, and the animals may become even more sensitive to Cd stress in subsequent generations. - Tolerance of sensitive Daphnia magna clones to cadmium was dependent on long-term or multigenerational exposure

Ecotoxicological evaluation of the short term effects of fresh and stabilized textile sludges before application in forest soil restoration

International Nuclear Information System (INIS)

Rosa, Edson V.C.; Giuradelli, Thayse M.; Correa, Albertina X.R.; Roerig, Leonardo R.; Schwingel, Paulo R.; Resgalla, Charrid; Radetski, Claudemir M.

2007-01-01

The short term (eco)toxicity potential of fresh and stabilized textile sludges, as well as the short term (eco)toxicity of leachates obtained from both fresh and stabilized textile sludges, was evaluated by a battery of toxicity tests carried out with bacteria, algae, daphnids, fish, earthworms, and higher plants. The (eco)toxicological results showed that, after 120 d of stabilization, the experimental loading ratio of 25% sludge:75% soil (v/v) (equivalent to 64.4 ton/ha) did not significantly increase toxicity effects and increased significantly the biomass yield for earthworms and higher plants. The rank of biological sensitivity endpoints was: Algae ∼ Plant biomass > Plant germination ∼ Daphnids > Bacteria ∼ Fish > Annelids. The lack of short term toxicity effects and the stimulant effect observed with higher plants and earthworms are good indications of the fertilizer/conditioner potential of this industrial waste, which after stabilization can be used in the restoration of a non-productive forest soil. - Short term ecotoxicity evaluation of textile sludge showed that stabilized sludge can be used in the restoration of a non-productive forest soil

Benthic macroinvertebrate assemblages and sediment toxicity testing in the Ely Creek watershed restoration project

International Nuclear Information System (INIS)

Soucek, D.J.; Currie, R.J.; Cherry, D.S.; Latimer, H.A.

1998-01-01

The Ely Creek watershed in Lee County, Virginia, contains an abundance of abandoned mined land (AML) seeps that contaminate the majority of the creek and its confluence into Big Stone Creek. Contaminated sediments had high concentrations of iron (∼10,000 mg/kg), aluminum (∼1,500 mg/kg), magnesium (∼400 mg/kg) and manganese (∼150 mg/kg). Copper and zinc generally ranged from 3 to 20 mg/kg. Benthic macroinvertebrates surveys at six of 20 sites sampled in the watershed yielded no macroinvertebrates, while eight others had total abundances of 1 to 9 organisms. Four reference sites contained ≥100 organisms and at least 14 different taxa. Laboratory, 10-day survival/impairment sediments tests with Daphnia magna did not support the field data. Mortality of 92 to 100% for D. magna occurred in samples collected from six cities. Daphnid reproduction was more sensitive than laboratory test organism survivorship; however, neither daphnid survivorship nor reproduction were good predictors of taxa richness. Laboratory test concerns included the use of a reference diluent water rather than site specific diluent water

Preliminary analysis of the transient overpower accident for CRBRP. Final report

International Nuclear Information System (INIS)

Kastenberg, W.E.; Frank, M.V.

1975-07-01

A preliminary analysis of the transient overpower accident for the Clinch River Breeder Reactor Plant (CRBRP) is presented. Several uncertainties in the analysis and the estimation of ramp rates during the transition to disassembly are discussed. The major conclusions are summarized

Soil bioretention protects juvenile salmon and their prey from the toxic impacts of urban stormwater runoff.

Science.gov (United States)

McIntyre, J K; Davis, J W; Hinman, C; Macneale, K H; Anulacion, B F; Scholz, N L; Stark, J D

2015-08-01

Green stormwater infrastructure (GSI), or low impact development, encompasses a diverse and expanding portfolio of strategies to reduce the impacts of stormwater runoff on natural systems. Benchmarks for GSI success are usually framed in terms of hydrology and water chemistry, with reduced flow and loadings of toxic chemical contaminants as primary metrics. Despite the central goal of protecting aquatic species abundance and diversity, the effectiveness of GSI treatments in maintaining diverse assemblages of sensitive aquatic taxa has not been widely evaluated. In the present study we characterized the baseline toxicity of untreated urban runoff from a highway in Seattle, WA, across six storm events. For all storms, first flush runoff was toxic to the daphniid Ceriodaphnia dubia, causing up to 100% mortality or impairing reproduction among survivors. We then evaluated whether soil media used in bioretention, a conventional GSI method, could reduce or eliminate toxicity to juvenile coho salmon (Oncorhynchus kisutch) as well as their macroinvertebrate prey, including cultured C. dubia and wild-collected mayfly nymphs (Baetis spp.). Untreated highway runoff was generally lethal to salmon and invertebrates, and this acute mortality was eliminated when the runoff was filtered through soil media in bioretention columns. Soil treatment also protected against sublethal reproductive toxicity in C. dubia. Thus, a relatively inexpensive GSI technology can be highly effective at reversing the acutely lethal and sublethal effects of urban runoff on multiple aquatic species. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

Chemical and toxicological characterization of slurry reactor biotreatment of explosives-contaminated soils

Energy Technology Data Exchange (ETDEWEB)

Griest, W.H.; Stewart, A.J.; Vass, A.A.; Ho, C.H.

1998-08-01

Treatment of 2,4,6-trinitrotoluene (TNT)-contaminated soil in the Joliet Army Ammunition Plant (JAAP) soil slurry bioreactor (SSBR) eliminated detectable TNT but left trace levels of residual monoamino and diamino metabolites under some reactor operating conditions. The reduction of solvent-extractable bacterial mutagenicity in the TNT-contaminated soil was substantial and was similar to that achieved by static pile composts at the Umatilla Army Depot Activity (UMDA) field demonstration. Aquatic toxicity to Ceriodaphnia dubia from TNT in the leachates of TNT-contaminated soil was eliminated in the leachates of JAAP SSBR product soil. The toxicity of soil product leachates to Ceriodaphnia dubia was reasonably predicted using the specific toxicities of the components detected, weighted by their leachate concentrations. In samples where TNT metabolites were observed in the soil product and its leachates, this method determined that the contribution to predicted toxicity values was dominated by trace amounts of the diamino-metabolites, which are very toxic to ceriodaphnia dubia. When the SSBR operating conditions reduced the concentrations of TNT metabolites in the product soils and their leachates to undetectable concentrations, the main contributors to predicted aquatic toxicity values appeared to be molasses residues, potassium, and bicarbonate. Potassium and bicarbonate are beneficial or benign to the environment, and molasses residues are substantially degraded in the environment. Exotoxins, pathogenic bacteria, inorganic particles, ammonia, and dissolved metals did not appear to be important to soil product toxicity.

Toxicity of six heterocyclic nitrogen compounds to Daphnia pulex

Science.gov (United States)

Perry, Cynthia M.; Smith, Stephen B.

1988-01-01

We determined the relative toxicities to the aquatic crustacean Daphniz pulex of six heterocyclic nitrogen compunds. These compounds were selected because they were detected in lake trout or walleyes and were commercially available. Stress to the daphnid populations may affect forage fish populations that depend either directly or indirectly on zooplankton as a food source in the Great Lakes.

Mechanical and physical properties of 2 1/4 Cr--1 Mo steel in support of CRBRP steam generator design

International Nuclear Information System (INIS)

Brinkman, C.R.; Williams, R.K.; Klueh, R.L.; Hebble, T.L.

1975-01-01

Mechanical and physical property tests on annealed 2 1 / 4 Cr-1 Mo steel were conducted in an effort to define behavior in support of the design of the Clinch River Breeder Reactor Plant (CRBRP) steam generator design. Interim empirical expressions and/or data are reported from the results of tensile, creep, fatigue, creep-fatigue, subcritical crack growth, thermal conductivity, thermal diffusivity, and thermal expansion tests and analysis. These expressions cover behavior, where appropriate, over a range of temperatures from 25 to as high as 700 0 C. Comparisons between thermal conductivity and diffusivity values and those found in the American Society of Mechanical Engineers (ASME) Code indicated that the new values were significantly higher than those found presently in the Code. The importance and complexity of obtaining valid mechanical and physical properties for the Clinch River Breeder Reactor Plant (CRBRP) steam generator are discussed. (U.S.)

Insect lipid profile: aqueous versus organic solvent-based extraction methods

NARCIS (Netherlands)

Tzompa Sosa, D.A.; Yi, L.; Valenberg, van H.J.F.; Boekel, van M.A.J.S.; Lakemond, C.M.M.

2014-01-01

In view of future expected industrial bio-fractionation of insects, we investigated the influence of extraction methods on chemical characteristics of insect lipids. Lipids from Tenebrio molitor, Alphitobius diaperinus, Acheta domesticus and Blaptica dubia, reared in the Netherlands, were extracted

Reactor enclosure. BRC meeting presentation

International Nuclear Information System (INIS)

Fisch, J.W.

1975-01-01

The latest status of key components of the Reactor Enclosure System of the Clinch River Breeder Reactor Plant is described. Areas where there have been notable design changes or significant design detail maturity in the six months since the last BRC presentation are highlighted. (auth)

Extraction and characterisation of protein fractions from five insect species

NARCIS (Netherlands)

Yi, L.; Lakemond, C.M.M.; Sagis, L.M.C.; Eisner-Schadler, V.R.; Huis, van A.; Boekel, van M.A.J.S.

2013-01-01

Tenebrio molitor, Zophobas morio, Alphitobius diaperinus, Acheta domesticus and Blaptica dubia were evaluated for their potential as a future protein source. Crude protein content ranged from 19% to 22% (Dumas analysis). Essential amino acid levels in all insect species were comparable with soybean

The responses of South African patellid limpets to invertebrate ...

African Journals Online (AJOL)

The starfish Marthasterias glacialis is a generalized predator, feeding particularly on Choromytilus meridionalis, but also on several limpets, notably Patella longicosta. Thais dubia (Gastropoda) feeds mainly on barnacles, mussels, and Patella granulans. The gastropods Burnupena delalandii and B. cincta are principally ...

Feasibility of constructed wetlands for removing chlorothalonil and chlorpyrifos from aqueous mixtures

International Nuclear Information System (INIS)

Sherrard, R.M.; Bearr, J.S.; Murray-Gulde, C.L.; Rodgers, J.H.; Shah, Y.T.

2004-01-01

Chlorpyrifos (an insecticide) and chlorothalonil (a fungicide) are transported in stormwater runoff and can be lethal to receiving aquatic system biota. This study determined removal rates of chlorpyrifos and chlorothalonil in simulated stormwater runoff treated in constructed wetland mesocosms. Using sentinel species, Ceriodaphnia dubia and Pimephales promelas, observed declines in toxicity of the simulated runoff after treatment were 98 and 100%, respectively. First order removal rates were 0.039/h for chlorpyrifos and 0.295/h for chlorothalonil in these experiments. Constructed wetland mesocosms were effective for decreasing concentrations of chlorpyrifos and chlorothalonil in simulated stormwater runoff, and decreasing P. promelas and C. dubia mortality resulting from these exposures. The results from this study indicate that constructed wetlands could be part of an efficient mitigation strategy for stormwater runoff containing these pesticides. - Constructed wetlands have potential for treatment of pesticide mixtures in stormwater runoff

Feasibility of constructed wetlands for removing chlorothalonil and chlorpyrifos from aqueous mixtures

Energy Technology Data Exchange (ETDEWEB)

Sherrard, R.M.; Bearr, J.S.; Murray-Gulde, C.L.; Rodgers, J.H.; Shah, Y.T

2004-02-01

Chlorpyrifos (an insecticide) and chlorothalonil (a fungicide) are transported in stormwater runoff and can be lethal to receiving aquatic system biota. This study determined removal rates of chlorpyrifos and chlorothalonil in simulated stormwater runoff treated in constructed wetland mesocosms. Using sentinel species, Ceriodaphnia dubia and Pimephales promelas, observed declines in toxicity of the simulated runoff after treatment were 98 and 100%, respectively. First order removal rates were 0.039/h for chlorpyrifos and 0.295/h for chlorothalonil in these experiments. Constructed wetland mesocosms were effective for decreasing concentrations of chlorpyrifos and chlorothalonil in simulated stormwater runoff, and decreasing P. promelas and C. dubia mortality resulting from these exposures. The results from this study indicate that constructed wetlands could be part of an efficient mitigation strategy for stormwater runoff containing these pesticides. - Constructed wetlands have potential for treatment of pesticide mixtures in stormwater runoff.

U.S. LMFBR steam generators materials considerations and waterside chemistry issues

Energy Technology Data Exchange (ETDEWEB)

Spalaris, C N

1975-07-01

This report describes the materials and waterside chemistry topics most relevant to the steam generator system for the Clinch River Breeder Reactor Plant. Development programs necessary to support or confirm design and plant operating conditions are summarized, together with selected test results obtained to date. (author)

U.S. LMFBR steam generators materials considerations and waterside chemistry issues

International Nuclear Information System (INIS)

Spalaris, C.N.

1975-01-01

This report describes the materials and waterside chemistry topics most relevant to the steam generator system for the Clinch River Breeder Reactor Plant. Development programs necessary to support or confirm design and plant operating conditions are summarized, together with selected test results obtained to date. (author)

Posterior vaginal wall Gartner′s duct cyst

Directory of Open Access Journals (Sweden)

Ripan Bala

2015-01-01

Full Text Available Cyst of posterior vaginal wall is very rare. This case relates to a patient who presented with polypoidal mass protruding out from vagina which could have been easily mistaken as uterovaginal prolapse, but appropriate clinical evaluation supported with investigations clinched the diagnosis easily.

76 FR 62259 - Endangered and Threatened Wildlife and Plants; Partial 90-Day Finding on a Petition To List 404...

Science.gov (United States)

2011-10-06

... electronic mail, a petition from the Center for Biological Diversity (CBD), Alabama Rivers Alliance, Clinch... electronic mail to Noah Greenwald at CBD, we acknowledged receipt of the Petition. On May 10, 2010, we... the petition. Crystal Darter (Crystallaria asprella) The crystal darter is a slender, cigar-shaped...

First annual report on the Biological Monitoring and Abatement Program at Oak Ridge National Laboratory

International Nuclear Information System (INIS)

Loar, J.M.; Amano, H.; Jimenez, B.D.; Kitchings, J.T.; Meyers-Schoene, L.; Mohrbacher, D.A.; Olsen, C.R.

1992-08-01

As a condition of the National Pollutant Discharge Elimination System (NPDES) permit issued to Oak Ridge National Laboratory (ORNL) on April 1, 1986, a Biological Monitoring and Abatement Program (BMAP) was developed for White Oak Creek (WOC); selected tributaries of WOC, including Fifth Creek, First Creek, Melton Branch, and Northwest Tributary; and the Clinch River. BMAP consists of seven major tasks that address both radiological and nonradiological contaminants in the aquatic and terrestrial environs on-site and the aquatic environs off-site. These tasks are (1) toxicity monitoring; (2) bioaccumulation monitoring of nonradiological contaminants in aquatic biota; (3) biological indicator studies; (4) instream ecological monitoring; (5) assessment of contaminants in the terrestrial environment; (6) radioecology of WOC and White Oak Lake (WOL); and (7) contaminant transport, distribution, and fate in the WOC embayment-Clinch River-Watts Bar Reservoir system. This document, the first of a series of annual reports presenting the results of BMAP, describes studies that were conducted from March through December 1986

Recommendations on the proposed Monitored Retrievable Storage Facility

Energy Technology Data Exchange (ETDEWEB)

1985-10-01

Following the Department of Energy's announcement in April 1985 that three Tennessee sites were to be considered for the Monitored Retrievable Storage facility, Governor Lamar Alexander initiated a review of the proposal to be coordinated by his Safe Growth Team. Roane County and the City of Oak Ridge, the local governments sharing jurisdiction over DOE's primary and secondary sites, were invited to participate in the state's review of the MRS proposal. Many issues related to the proposed MRS are being considered by the Governor's Safe Growth Team. The primary objective of the Clinch River MRS Task Force has been to determine whether the proposed Monitored Retrievable Storage facility should be accepted by the local governments, and if so, under what conditions. The Clinch River MRS Task Force is organized into an Executive Committee cochaired by the Roane County Executive and Mayor of Oak Ridge and three Study Groups focusing on environmental (including health and safety), socioeconomic, and transportation issues.

Recommendations on the proposed Monitored Retrievable Storage Facility

Energy Technology Data Exchange (ETDEWEB)

1985-10-01

Following the Department of Energy`s announcement in April 1985 that three Tennessee sites were to be considered for the Monitored Retrievable Storage facility, Governor Lamar Alexander initiated a review of the proposal to be coordinated by his Safe Growth Team. Roane County and the City of Oak Ridge, the local governments sharing jurisdiction over DOE`s primary and secondary sites, were invited to participate in the state`s review of the MRS proposal. Many issues related to the proposed MRS are being considered by the Governor`s Safe Growth Team. The primary objective of the Clinch River MRS Task Force has been to determine whether the proposed Monitored Retrievable Storage facility should be accepted by the local governments, and if so, under what conditions. The Clinch River MRS Task Force is organized into an Executive Committee cochaired by the Roane County Executive and Mayor of Oak Ridge and three Study Groups focusing on environmental (including health and safety), socioeconomic, and transportation issues.

Flow Induced Vibration Program at Argonne National Laboratory

Science.gov (United States)

1984-01-01

The Argonne National Laboratory's Flow Induced Vibration Program, currently residing in the Laboratory's Components Technology Division is discussed. Throughout its existence, the overall objective of the program was to develop and apply new and/or improved methods of analysis and testing for the design evaluation of nuclear reactor plant components and heat exchange equipment from the standpoint of flow induced vibration. Historically, the majority of the program activities were funded by the US Atomic Energy Commission, the Energy Research and Development Administration, and the Department of Energy. Current DOE funding is from the Breeder Mechanical Component Development Division, Office of Breeder Technology Projects; Energy Conversion and Utilization Technology Program, Office of Energy Systems Research; and Division of Engineering, Mathematical and Geosciences, office of Basic Energy Sciences. Testing of Clinch River Breeder Reactor upper plenum components was funded by the Clinch River Breeder Reactor Plant Project Office. Work was also performed under contract with Foster Wheeler, General Electric, Duke Power Company, US Nuclear Regulatory Commission, and Westinghouse.

First annual report on the Biological Monitoring and Abatement Program at Oak Ridge National Laboratory

Energy Technology Data Exchange (ETDEWEB)

Loar, J. M. [ed.; Adams, S. M.; Blaylock, B. G.; Boston, H. L.; Frank, M. L.; Garten, C. T.; Houston, M. A.; Kimmel, B. L.; Ryon, M. G.; Smith, J. G.; Southworth, G. R.; Stewart, A. J.; Walton, B. T.; Berry, J. B.; Talmage, S. S. [Oak Ridge National Lab., TN (United States); Amano, H. [JAERI, Tokai Res., Establishment, Ibari-Ken (Japan); Jimenez, B. D. [School of Pharmacy, Univ. of Puerto Rico (San Juan); Kitchings, J. T. [ERCE, Denver, CO (United States); Meyers-Schoene, L. [Advanced Sciences, Inc., Fernald, OH (United States); Mohrbacher, D. A. [Univ. of Tennessee, Knoxville, TN (United States); Olsen, C. R. [USDOE Office of Energy Research, Washington, DC (United States). Office of Health and Environmental Research

1992-08-01

As a condition of the National Pollutant Discharge Elimination System (NPDES) permit issued to Oak Ridge National Laboratory (ORNL) on April 1, 1986, a Biological Monitoring and Abatement Program (BMAP) was developed for White Oak Creek (WOC); selected tributaries of WOC, including Fifth Creek, First Creek, Melton Branch, and Northwest Tributary; and the Clinch River. BMAP consists of seven major tasks that address both radiological and nonradiological contaminants in the aquatic and terrestrial environs on-site and the aquatic environs off-site. These tasks are (1) toxicity monitoring; (2) bioaccumulation monitoring of nonradiological contaminants in aquatic biota; (3) biological indicator studies; (4) instream ecological monitoring; (5) assessment of contaminants in the terrestrial environment; (6) radioecology of WOC and White Oak Lake (WOL); and (7) contaminant transport, distribution, and fate in the WOC embayment-Clinch River-Watts Bar Reservoir system. This document, the first of a series of annual reports presenting the results of BMAP, describes studies that were conducted from March through December 1986.

A taxonomic revision of the new world species of Sirthenea (Heteroptera: Reduviidae: Peiratinae)

NARCIS (Netherlands)

Willemse, L.

1985-01-01

The American species of Sirthenea are revised and keys to the 12 species and seven subspecies are given. Four new species and two new subspecies are described viz., S. ater (Brazil: Minas Geraes), S. dubia (Panama; Paraguay: Caaguazu. Argentina: Misiones; Entre Rios), S. ferdinandi (Argentina:

Assessment of the effects of the carbamazepine on the endogenous endocrine system of Daphnia magna.

Science.gov (United States)

Oropesa, A L; Floro, A M; Palma, P

2016-09-01

In the present study, the endocrine activity of the antiepileptic pharmaceutical carbamazepine (CBZ) in the crustacean Daphnia magna was assessed. To assess the hormonal activity of the drug, we exposed maternal daphnids and embryos to environmental relevant concentrations of CBZ (ranging from 10 to 200 μg/L) and to mixtures of CBZ with fenoxycarb (FEN; 1 μg/L). Chronic exposure to CBZ significantly decreased the reproductive output and the number of molts of D. magna at 200 μg/L. This compound induced the production of male offspring (12 ± 1.7 %), in a non-concentration-dependent manner, acting as a weak juvenile hormone analog. Results showed that this substance, at tested concentrations, did not antagonize the juvenoid action of FEN. Further, CBZ has shown to be toxic to daphnid embryos through maternal exposure interfering with their normal gastrulation and organogenesis stages but not producing direct embryo toxicity. These findings suggest that CBZ could act as an endocrine disruptor in D. magna as it decreases the reproductive output, interferes with sex determination, and causes development abnormality in offspring. Therefore, CBZ could directly affect the population sustainability.

Gene transcription profiles, global DNA methylation and potential transgenerational epigenetic effects related to Zn exposure history in Daphnia magna

International Nuclear Information System (INIS)

Vandegehuchte, Michiel B.; De Coninck, Dieter; Vandenbrouck, Tine; De Coen, Wim M.; Janssen, Colin R.

2010-01-01

A reduced level of DNA methylation has recently been described in both Zn-exposed and non-exposed offspring of Daphnia magna exposed to Zn. The hypothesis examined in this study is that DNA hypomethylation has an effect on gene transcription. A second hypothesis is that accumulative epigenetic effects can affect gene transcription in non-exposed offspring from parents with an exposure history of more than one generation. Transcriptional gene regulation was studied with a cDNA microarray. In the exposed and non-exposed hypomethylated daphnids, a large proportion of common genes were similarly up- or down-regulated, indicating a possible effect of the DNA hypomethylation. Two of these genes can be mechanistically involved in DNA methylation reduction. The similar transcriptional regulation of two and three genes in the F 0 and F 1 exposed daphnids on one hand and their non-exposed offspring on the other hand, could be the result of a one-generation temporary transgenerational epigenetic effect, which was not accumulative. - Zn-induced DNA hypomethylation is related to gene transcription in Daphnia magna and Zn exposure potentially induced limited temporary transgenerational effects on gene transcription.

Elimination of Whole Effluent Toxicity NPDES Permit Limits through the Use of an Alternative Testing Species and Reasonable Potential Analysis

International Nuclear Information System (INIS)

PAYNE, W.L.

2004-01-01

The cladoceran, Ceriodaphnia dubia (C. dubia), is required by the State of South Carolina to be used in whole effluent toxicity (WET) compliance tests in order to meet limits contained within National Pollutant Discharge Elimination System (NPDES) permits. Westinghouse Savannah River Company (WSRC) experienced WET test failures for no clear reason over a long period of time. Toxicity identification examinations on effluents did not indicate the presence of toxicants; therefore, the WET test itself was brought under suspicion. Research was undertaken with an alternate cladoceran, Daphnia ambigua (D. ambigua). It was determined that this species survives better in soft water, so approval was obtained from regulating authorities to use this ''alternate'' species in WET tests. The result was better test results and elimination of non-compliances. The successful use of D. ambigua allowed WSRC to gain approval from the South Carolina Department of Health and Environmental Control (SCDHEC) to remove WET limits from the NPDES permit

NMDA receptor activation upstream of methyl farnesoate signaling for short day-induced male offspring production in the water flea, Daphnia pulex.

Science.gov (United States)

Toyota, Kenji; Miyakawa, Hitoshi; Yamaguchi, Katsushi; Shigenobu, Shuji; Ogino, Yukiko; Tatarazako, Norihisa; Miyagawa, Shinichi; Iguchi, Taisen

2015-03-14

The cladoceran crustacean Daphnia pulex produces female offspring by parthenogenesis under favorable conditions, but in response to various unfavorable external stimuli, it produces male offspring (environmental sex determination: ESD). We recently established an innovative system for ESD studies using D. pulex WTN6 strain, in which the sex of the offspring can be controlled simply by changes in the photoperiod: the long-day and short-day conditions can induce female and male offspring, respectively. Taking advantage of this system, we demonstrated that de novo methyl farnesoate (MF) synthesis is necessary for male offspring production. These results indicate the key role of innate MF signaling as a conductor between external environmental stimuli and the endogenous male developmental pathway. Despite these findings, the molecular mechanisms underlying up- and downstream signaling of MF have not yet been well elucidated in D. pulex. To elucidate up- and downstream events of MF signaling during sex determination processes, we compared the transcriptomes of daphnids reared under the long-day (female) condition with short-day (male) and MF-treated (male) conditions. We found that genes involved in ionotropic glutamate receptors, known to mediate the vast majority of excitatory neurotransmitting processes in various organisms, were significantly activated in daphnids by the short-day condition but not by MF treatment. Administration of specific agonists and antagonists, especially for the N-methyl-D-aspartic acid (NMDA) receptor, strongly increased or decreased, respectively, the proportion of male-producing mothers. Moreover, we also identified genes responsible for male production (e.g., protein kinase C pathway-related genes). Such genes were generally shared between the short-day reared and MF-treated daphnids. We identified several candidate genes regulating ESD which strongly suggests that these genes may be essential factors for male offspring production as an

FLOODPLAIN, CLINCH COUNTY, GA AND INCORPORATED AREAS

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — he Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

The Effects of Natural and Anthropogenic Microparticles on Individual Fitness in Daphnia magna.

Directory of Open Access Journals (Sweden)

Martin Ogonowski

Full Text Available Concerns are being raised that microplastic pollution can have detrimental effects on the feeding of aquatic invertebrates, including zooplankton. Both small plastic fragments (microplastics, MPs produced by degradation of larger plastic waste (secondary MPs; SMPs and microscopic plastic spheres used in cosmetic products and industry (primary MPs; PMPs are ubiquitously present in the environment. However, despite the fact that most environmental MPs consist of weathered plastic debris with irregular shape and broad size distribution, experimental studies of organism responses to MP exposure have largely used uniformly sized spherical PMPs. Therefore, effects observed for PMPs in such experiments may not be representative for MP-effects in situ. Moreover, invertebrate filter-feeders are generally well adapted to the presence of refractory material in seston, which questions the potential of MPs at environmentally relevant concentrations to measurably affect digestion in these organisms. Here, we compared responses to MPs (PMPs and SMPs and naturally occurring particles (kaolin clay using the cladoceran Daphnia magna as a model organism. We manipulated food levels (0.4 and 9 μg C mL-1 and MP or kaolin contribution to the feeding suspension (<1 to 74% and evaluated effects of MPs and kaolin on food uptake, growth, reproductive capacity of the daphnids, and maternal effects on offspring survival and feeding. Exposure to SMPs caused elevated mortality, increased inter-brood period and decreased reproduction albeit only at high MP levels in the feeding suspension (74% by particle count. No such effects were observed in either PMP or kaolin treatments. In daphnids exposed to any particle type at the low algal concentration, individual growth decreased by ~15%. By contrast, positive growth response to all particle types was observed at the high algal concentration with 17%, 54% and 40% increase for kaolin, PMP and SMP, respectively. When test particles

Temporomandibular Joint Disorders – A Few Interesting Experiences to Share

Directory of Open Access Journals (Sweden)

Sumit Chattopadhyay

2015-08-01

Full Text Available Temporomandibular joint disorders rarely present in a very direct way. Often, the complaints are far away from the joint itself, making the diagnosis difficult. But a high index of suspicion and a good clinical idea often clinches the diagnosis in many cases of otalgia with apparently no definitive finding.

History of the water chemistry for the few tube test model

International Nuclear Information System (INIS)

Moss, S.A.; Simpson, J.L.

1979-09-01

The water chemistry activities carried out in support of the Few Tube Test are described. This test was conducted to provide design confirmation data for the Clinch River Breeder Reactor Project (CRBRP) steam generators. Proposed CRBRP chemistry was followed; all volatile treatment (AVT) of water was carried out with on-line monitoring capability

Competencia en Cladocera: implicancias de la sobreposición en el uso de los recursos tróficos Competition in Cladocera: implications of the trophic resources use overlap

Directory of Open Access Journals (Sweden)

GINGER MARTINEZ

2000-12-01

Full Text Available A pesar que numerosa evidencia demuestra el uso de diferentes estrategias de alimentación entre cladóceros, la clásica descripción de una conducta alimentaria pasiva ha conducido a una subestimación de la sobreposición de nicho trófico sobre sus patrones de coexistencia. En condiciones de microcosmos, se mantuvieron experimentos pareados de dinámica poblacional de los cladóceros Moina micrura (Moinidae y Ceriodaphnia dubia (Daphnidae, especializados en los recursos Chlorella sp. y Oocystis sp. (Chlorococcales respectivamente y del generalista Daphnia ambigua (Daphnidae, durante 35 - 45 días. Mientras que los especialistas M. micrura y C. dubia no dieron evidencias de competencia, la presencia de cada uno produjo un significativo decrecimiento de la densidad del generalista D. ambigua, ocasionando en algunos casos su extinción. A pesar que las dinámicas poblacionales de cada especialista fueron afectadas cualitativamente por la presencia del generalista, no hubo efecto de esta especie sobre las densidades, resultando una interacción asimétrica entre cada especialista con el generalista. Estos resultados demuestran que la conducta alimentaria y la sobreposición en el uso de los recursos serían condiciones determinantes en la composición de ensambles de cladócerosAlthough numerous evidence reveals the use of different feeding strategies among cladocerans, the impact of trophic niche overlap on their patterns of coexistence has been underestimated due to the classical description of passive feeding behavior. Under microcosm conditions, paired experiments of population dynamics were conducted for the cladocerans Moina micrura (Moinidae and Ceriodaphnia dubia (Daphnidae, specialists on the resources Chlorella sp. and Oocystis sp. (Chlorococcales respectively, and for the generalist species Daphnia ambigua (Daphnidae, during 35 - 45 days. While, the specialists M. micrura and C. dubia did not show evidences of competition, their

Ecological risk assessment in a large river-reservoir. 8: Experimental study of the effects of polychlorinated biphenyls on reproductive success in mink

International Nuclear Information System (INIS)

Halbrook, R.S.; Aulerich, R.J.; Bursian, S.J.; Lewis, L.

1999-01-01

As a component of an ecological risk assessment of Poplar Creek (located on the Oak Ridge Reservation [ORR]) and the Clinch River (a large river-reservoir system), fish from Poplar Creek, the Clinch River, and Atlantic Ocean were fed to ranch mink to evaluate reproductive success. Five diets, each composed of 75% fish and 25% normal ranch mink chow, were prepared. Two diets served as reference diets and contained 75% Atlantic Ocean fish or 75% Clinch River fish collected above the ORR. The fish portion of the remaining three diets contained 25, 50, and 75% fish collected from Poplar Creek and 50, 25, and 0% ocean fish, respectively. Five mink groups (eight females and two males each) were each fed one of the prepared diets for 196 days. Polychlorinated biphenyl concentrations were determined in diets and various mink tissues, ethoxyresorufin-O-deethylase (EROD) activity was determined in liver tissue, and reproductive success was evaluated. Concentrations of PCB were greatest in the diet composed of 75% Poplar Creek fish and in tissues from mink fed this diet and their offspring. There was a trend toward decreased adult female and kit weights and reduced mean litter size in mink fed diets containing 75% Poplar Creek fish; however, at 6 weeks of age, kit survival was similar among diet groups. Liver EROD activity significantly increased in adult female mink fed 50 and 75% Poplar Creek fish diets. Estimated dietary concentrations of PCBs were similar to or slightly lower than concentrations associated with adverse effects in experimentally dosed mink. Mercury (Hg) concentrations previously reported in these same mink were below that associated with adverse effects, and there was no indication of additive or synergistic effects from exposure to PCBs plus Hg. It is unlikely that population-level reproductive effects would be observed in mink consuming fish from Poplar Creek on the ORR

Motor actions and spatiotemporal changes by weight divisions of mixed martial arts: Applications for training.

Science.gov (United States)

Miarka, Bianca; Brito, Ciro José; Bello, Fábio Dal; Amtmann, John

2017-10-01

This study compared motor actions and spatiotemporal changes between weight divisions from Ultimate Fighting Championship (UFC™), conducting a practical application for mixed martial arts (MMA) training. For this, we analyzed 2814 rounds of all weight divisions by motor actions and spatiotemporal changes according actions and time of the Keeping distance, Clinch and Groundwork combat phases. We observed differences between weight divisions in the keeping distance on stand-up combat (p≤0.001; with lower time in Featherweight 131.4s and bantamweight 127.9s) clinch without attack (p≤0.001; with higher timer in Flyweight 11.4s and Half-middleweight 12.6s) and groundwork without attack (p≤0.001; with higher timer in Half-middleweight 0.9s). During keeping distance, half-middleweight presented a higher frequency of Head Strikes Landed (p=0.026; 7±8 times) and attempted (p=0.003; 24±22 times). In clinch actions heavyweight present a higher frequency (p≤0.023) of head strike landed (3±7 times) and attempted (4±9 times) and half-middleweight for body strikes (p≤0.023) landed (2±5 times) and attempted (3±5 times). At the last, during groundwork, Bantamweight present a higher frequency (p≤0.036) of head strikes landed (8±10 times) and attempted (10±13 times) body strikes landed (p≤0.044; 3±5 times) and attempted (3±6 times). This study reveals important point to training and provide a challenge applied referential to the conditioning plains. From the weight divisions differences should be aware of the increase in the frequency of distance actions, especially in light and middleweights. On the Ground, bantamweight can focus on striking and grappling actions than others. Copyright © 2017 Elsevier B.V. All rights reserved.

A young woman with endometriosis of kidney

International Nuclear Information System (INIS)

Dutta, P.; Bhat, M. H.; Bhansali, A.; Kumar, V.

2006-01-01

Endometriosis of kidney is a rare manifestation of a relatively common disease. We report a case in which ovarian and renal endometriosis were diagnosed concurrently. The disease was probably silent for a long time due to coexistent thyrotoxicosis modifying estrogen metabolism. Fine needle aspiration cytology clinched the diagnosis of endometriosis and avoided unnecessary nephrectomy. (author)

Biological Monitoring and Abatement Program for the Oak Ridge K-25 Site

International Nuclear Information System (INIS)

Kszos, L.A.; Adams, S.M.; Ashwood, T.L.; Blaylock, B.G.; Greeley, M.S.; Loar, J.M.; Peterson, M.J.; Ryon, M.G.; Smith, J.G.; Southworth, G.R.; Shoemaker, B.A.; Hinzman, R.L.

1993-02-01

A proposed Biological Monitoring and Abatement Program (BMAP) for the Oak Ridge K-25 Site was prepared in December 1992 as required by the renewed National Pollutant Discharge Elimination System permit that was issued on October 1, 1992. The proposed BMAP consists of four tasks that reflect different but complementary approaches to evaluating the effects of K-25 Site effluents on the ecological integrity of Mitchell Branch, Poplar Creek, and the Poplar Creek embayment of the Clinch River. These tasks include (1) ambient toxicity monitoring, (2) bioaccumulation monitoring, (3) assessment of fish health, and (4) instream monitoring of biological communities. This overall BMAP plan combines established protocols with current biological monitoring techniques to assess environmental compliance and quantify ecological recovery. The BMAP will also determine whether the effluent limits established for the K-25 Site protect the designated use of the receiving streams (Mitchell Branch, Poplar Creek, and Clinch River) for growth and propagation of fish and other aquatic life. Results obtained from this biological monitoring program will also be used to document the ecological effects (and effectiveness) of remedial actions

Biological Monitoring and Abatement Program for the Oak Ridge K-25 Site

Energy Technology Data Exchange (ETDEWEB)

Kszos, L. A.; Adams, S. M.; Ashwood, T. L.; Blaylock, B. G.; Greeley, M. S.; Loar, J. M.; Peterson, M. J.; Ryon, M. G.; Smith, J. G.; Southworth, G. R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Div.; Shoemaker, B. A. [Oak Ridge K-25 Site, TN (United States); Hinzman, R. L. [Oak Ridge Research Inst., TN (United States)

1993-02-01

A proposed Biological Monitoring and Abatement Program (BMAP) for the Oak Ridge K-25 Site was prepared in December 1992 as required by the renewed National Pollutant Discharge Elimination System permit that was issued on October 1, 1992. The proposed BMAP consists of four tasks that reflect different but complementary approaches to evaluating the effects of K-25 Site effluents on the ecological integrity of Mitchell Branch, Poplar Creek, and the Poplar Creek embayment of the Clinch River. These tasks include (1) ambient toxicity monitoring, (2) bioaccumulation monitoring, (3) assessment of fish health, and (4) instream monitoring of biological communities. This overall BMAP plan combines established protocols with current biological monitoring techniques to assess environmental compliance and quantify ecological recovery. The BMAP will also determine whether the effluent limits established for the K-25 Site protect the designated use of the receiving streams (Mitchell Branch, Poplar Creek, and Clinch River) for growth and propagation of fish and other aquatic life. Results obtained from this biological monitoring program will also be used to document the ecological effects (and effectiveness) of remedial actions.

Mercury issues related to NPDES and the CERCLA watershed project at the Oak Ridge Y-12 Plant

International Nuclear Information System (INIS)

1996-11-01

The purpose of this document is to present the current understanding of the issues and options surrounding compliance with the current National Pollutant Discharge Elimination System (NPDES) permit conditions. This is a complicated issue that directly impacts, and will be directly impacted by, ongoing CERCLA activities in Lower East Fork Poplar Creek and the Clinch River/Poplar Creek. It may be necessary to reconstitute the whole and combine actions and decisions regarding the entire creek (origin to confluence with the Clinch River) to develop a viable long-term strategy that meets regulatory goals and requirements as well as those of DOE's 10-Year Plan and the new watershed management permitting approach. This document presents background information on the Reduction of Mercury in Plant Effluents (RMPE) and NPDES programs insofar as it is needed to understand the issues and options. A tremendous amount of data has been collected to support the NPDES/RMPE and CERCLA programs. These data are not presented, although they may be referenced and conclusions based on them may be presented, as necessary, to support discussion of the options

Flow Induced Vibration Program at Argonne National Laboratory

International Nuclear Information System (INIS)

1984-01-01

Argonne National Laboratory has had a Flow Induced Vibration Program since 1967; the Program currently resides in the Laboratory's Components Technology Division. Throughout its existence, the overall objective of the program has been to develop and apply new and/or improved methods of analysis and testing for the design evaluation of nuclear reactor plant components and heat exchange equipment from the standpoint of flow induced vibration. Historically, the majority of the program activities have been funded by the US Atomic Energy Commission (AEC), Energy Research and Development Administration (ERDA), and Department of Energy (DOE). Current DOE funding is from the Breeder Mechanical Component Development Division, Office of Breeder Technology Projects; Energy Conversion and Utilization Technology (ECUT) Program, Office of Energy Systems Research; and Division of Engineering, Mathematical and Geosciences, Office of Basic Energy Sciences. Testing of Clinch River Breeder Reactor upper plenum components has been funded by the Clinch River Breeder Reactor Plant (CRBRP) Project Office. Work has also been performed under contract with Foster Wheeler, General Electric, Duke Power Company, US Nuclear Regulatory Commission, and Westinghouse

Ecological risk assessment in a large river-reservoir. 2: Fish community

International Nuclear Information System (INIS)

Suter, G.W. II; Barnthouse, L.W.; Efroymson, R.A.; Jager, H.

1999-01-01

This paper summarizes the assessment of risks to fishes in the Clinch River Operable Unit due to contaminants released by the US Department of Energy's activities on its Oak Ridge Reservation in Tennessee. This paper focuses on the most contaminated area, the Poplar Creek (PC) embayment. The assessment is of interest because of its use of five distinct lines of evidence: fish community surveys, fish body burdens, toxicity tests of ambient waters, suborganismal bioindicators, and single chemical toxicity tests. None of these lines of evidence provided unambiguous evidence of a significant risk, but the surveys indicated that the fish community in PC was depauperate, polychlorinated biphenyl body burdens may have been at toxic levels in catfish, one of the three tests of ambient water showed clear toxicity, some of the indicators were indicative of toxic effects, and concentrations that have been toxic in the laboratory were detected periodically. Interpretation was further complicated by upstream contamination of both the Clinch River and PC. The risk characterization was performed by evaluating each line of evidence separately and then weighing the evidence using an ecoepidemiological approach

P{owering 'Trees

Indian Academy of Sciences (India)

Melia dubia Cav. of Meliaceae is a large deciduous tree. Leaves are compound with toothed leaflets. Flowers are small, greenish-yellow in much-branched inflorescences. Fruits are green, ellipsoidal with a single seed covered by hard portion ( as in a mango fruit) and surrounded by fleshy pulp outside. The bark is bitter ...

Metabolomics reveals an involvement of pantothenate for male production responding to the short-day stimulus in the water flea, Daphnia pulex.

Science.gov (United States)

Toyota, Kenji; Gavin, Alex; Miyagawa, Shinichi; Viant, Mark R; Iguchi, Taisen

2016-04-26

Under favorable conditions, the micro-crustacean Daphnia pulex produces female offspring by parthenogenesis, whereas under unfavorable conditions, they produce male offspring to induce sexual reproduction (environmental sex determination: ESD). We recently established a suitable system for ESD studies using D. pulex WTN6 strain, in which the sex of the offspring can be regulated by alterations in day-length; long-day and short-day conditions can induce female and male offspring, respectively. Taking advantage of this system, we have already demonstrated that methyl farnesoate (MF) synthesis is necessary for male offspring production, and identified ionotropic glutamate receptors as an upstream regulator of MF signaling. Despite these findings, the molecular mechanisms associated with MF signaling have not yet been well elucidated. In this study, we analyzed the whole metabolic profiles of mother daphnids reared under long-day (female-producing) and short-day (male-producing) conditions, and discovered that pantothenate (vitamin B5), a known precursor to coenzyme A, was significantly accumulated in response to the short-day condition. To confirm the innate role of pantothenate in D. pulex, this metabolite was administered to mother daphnids resulting in a significantly increased proportion of male offspring producing mothers. This study provides novel insights of the metabolic mechanisms of the ESD system in D. pulex.

Incorporation of phenomenological uncertainties in probabilistic safety analysis - application to LMFBR core disruptive accident energetics

Energy Technology Data Exchange (ETDEWEB)

Najafi, B; Theofanous, T G; Rumble, E T; Atefi, B

1984-08-01

This report describes a method for quantifying frequency and consequence uncertainty distribution associated with core disruptive accidents (CDAs). The method was developed to estimate the frequency and magnitude of energy impacting the reactor vessel head of the Clinch River Breeder Plant (CRBRP) given the occurrence of hypothetical CDAs. The methodology is illustrated using the CRBR example.

Multiple linear regression models for predicting chronic aluminum toxicity to freshwater aquatic organisms and developing water quality guidelines.

Science.gov (United States)

DeForest, David K; Brix, Kevin V; Tear, Lucinda M; Adams, William J

2018-01-01

The bioavailability of aluminum (Al) to freshwater aquatic organisms varies as a function of several water chemistry parameters, including pH, dissolved organic carbon (DOC), and water hardness. We evaluated the ability of multiple linear regression (MLR) models to predict chronic Al toxicity to a green alga (Pseudokirchneriella subcapitata), a cladoceran (Ceriodaphnia dubia), and a fish (Pimephales promelas) as a function of varying DOC, pH, and hardness conditions. The MLR models predicted toxicity values that were within a factor of 2 of observed values in 100% of the cases for P. subcapitata (10 and 20% effective concentrations [EC10s and EC20s]), 91% of the cases for C. dubia (EC10s and EC20s), and 95% (EC10s) and 91% (EC20s) of the cases for P. promelas. The MLR models were then applied to all species with Al toxicity data to derive species and genus sensitivity distributions that could be adjusted as a function of varying DOC, pH, and hardness conditions (the P. subcapitata model was applied to algae and macrophytes, the C. dubia model was applied to invertebrates, and the P. promelas model was applied to fish). Hazardous concentrations to 5% of the species or genera were then derived in 2 ways: 1) fitting a log-normal distribution to species-mean EC10s for all species (following the European Union methodology), and 2) fitting a triangular distribution to genus-mean EC20s for animals only (following the US Environmental Protection Agency methodology). Overall, MLR-based models provide a viable approach for deriving Al water quality guidelines that vary as a function of DOC, pH, and hardness conditions and are a significant improvement over bioavailability corrections based on single parameters. Environ Toxicol Chem 2018;37:80-90. © 2017 SETAC. © 2017 SETAC.

An efficient protocol for in vitro organogenesis and antioxidant ...

African Journals Online (AJOL)

Total flavonoids and phenolic content in leaves of in vitro Melia dubia was 0.56 ± 0.8 mg quercitin equivalent (QE) and 2.97 ± 0.17 mg gallic acid equivalent (GAE) respectively. The antioxidant property was further assed through measurement of DPPH radical scavenging activity. The in-vitro regeneration protocol can be ...

2015 status of the Lake Ontario lower trophic levels

Science.gov (United States)

Holeck, Kristen T.; Rudstam, Lars G.; Hotaling, Christopher; McCullough, Russ D.; Lemon, Dave; Pearsall, Web; Lantry, Jana; Connerton, Michael J.; LaPan, Steve; Biesinger, Zy; Lantry, Brian F.; Walsh, Maureen; Weidel, Brian C.

2016-01-01

Offshore spring total phosphorus (TP) in 2015 was 4.2 μ g/L, the same as in 2014; this is lower than 2001 - 2013, but there is no significant time trend 2001 - 2015. Offshore soluble reactive phosphorus (SRP) was very low in 2015; Apr/May - Oct mean values were water column samples taken show a stable zooplankton biomass but changing community composition since 2010. Cyclopoids increased 2013 - 2015 and daphnids declined 2014 - 2015.

Evaluation of wetlands designed to transfer and transform selected metals in an aqueous matrix

International Nuclear Information System (INIS)

Hawkins, W.B.; Gillespie, W.B. Jr.; Rodgers, J.H. Jr.

1995-01-01

Constructed wetlands can be used as an alternative to traditional wastewater treatment. Two wetlands were constructed at a Louisiana petroleum refinery and were used to study transfers and transformations of selected metals (Zn, Pb, and Cu) in a refinery effluent. In order to optimize metal removal from the aqueous matrix and subsequently decrease metal bioavailability, the hydroperiod, hydrosoil, and vegetation were specifically selected and incorporated into the wetland design. To test the metal removal efficiency of the constructed wetlands, refinery effluent was amended with 4 mg Zn/L as ZnCl 2 for 150 d. From influent to effluent, average total recoverable and soluble zinc concentrations decreased by 41 and 72%, respectively. Toxicity tests (7 d) using Ceriodaphnia dubia and Pimephales promelas illustrated a decrease in zinc bioavailability. Average C. dubia survival increased from 0--73% as a result of wetland treatment; for P. promelas, the increase in average survival was 37--94%. Based upon this field experiment, constructed wetlands can be specifically designed for zinc removal and concomitant decreases in toxicity

Toxicity of chloride under winter low-flow conditions in an urban watershed in central Missouri, USA

Science.gov (United States)

Allert, Ann L.; Cole-Neal, Cavelle L.; Fairchild, James F.

2012-01-01

Deicers such as sodium chloride and calcium chloride are used to treat snow and ice on road surfaces and have been identified as potential stressors on aquatic life. Hinkson Creek is an urban stream on the Missouri 303(d) list of impaired waters and is classified as impaired due to urban non-point source pollution. A 7-day toxicity test using Ceriodaphnia dubia was conducted to assess the toxicity of stream water during snowmelt at seven sites within the Hinkson Creek watershed. Chloride concentrations at two sites (Site 6, 1252 mg Cl/L; Site 4, 301 mg Cl/L) exceeded the U.S. Environmental Protection Agency chronic criterion (230 mg Cl/L). Survival (30 %) and total reproduction (6.9 young/adult) of C. dubia at Site 6 was significantly lower than survival (100 %) and total reproduction (30.4 young/adult) at Site 1 (reference site). Results indicate that chloride concentrations are elevated above water-quality criteria and that chloride may be a significant chemical stressor for macroinvertebrate communities during winter low-flow conditions in the Hinkson Creek watershed.

Micropropagation of caçari under different nutritive culture media ...

African Journals Online (AJOL)

The caçari (Myrciaria dubia) is a native fruit tree from Amazon with high concentrations of vitamin C. This study aimed to adjust a culture medium that meets the nutritional needs for the in vitro development of caçari, evaluating the effect of different concentrations and nutritive culture media, antioxidant, and levels of agar and ...

Interim storage of sodium in ferritic steel tanks at ambient temperature

International Nuclear Information System (INIS)

Blackburn, L.D.

1994-01-01

Sodium tanks originally fabricated for elevated temperature service in the Clinch River Breeder Reactor Plant (CRBRP) will be used to store sodium removed from the Fast Flux Test Facility (FFTF) in the Sodium Storage Facility (SSF) at ambient temperature. This report presents an engineering review to confirm that protection against brittle fracture of the ferritic steel tanks is adequate for the intended service

Simulant-material experimental investigation of flow dynamics in the CRBR Upper-Core Structure

International Nuclear Information System (INIS)

Wilhelm, D.; Starkovich, V.S.; Chapyak, E.J.

1982-09-01

The results of a simulant-material experimental investigation of flow dynamics in the Clinch River Breeder Reactor (CRBR) Upper Core Structure are described. The methodology used to design the experimental apparatus and select test conditions is detailed. Numerous comparisons between experimental data and SIMMER-II Code calculations are presented with both advantages and limitations of the SIMMER modeling features identified

Economic Impact Forecast System (EIFS) II: User’s Manual. Updated Edition.

Science.gov (United States)

1984-05-01

AYtr ACo - bo * AYtr AIh - ih *rpv *ACh Ala = io* ACo AS - Zc * C*(1-ZmW Pm+ PC] APY (av / amv) * (ABVt /tbv72) ACRe - (Zaf+ %as)*sc*AS AGRo - (tp...ga 35031 mc kinley, nm 13027 brooks, ga 35033 mora, nm 13037 calhoun, ga 35039 rio arriba , nm 13061 clay, ga 35043 sandoval, nm 13065 clinch, ga 35045

Unconventional abdominal uses of FIESTA (CISS) sequence

International Nuclear Information System (INIS)

Sen, Anitha

2013-01-01

The ability to provide cross-sectional imaging, combined with a lack of ionizing radiation has made magnetic resonance imaging (MRI) of abdomen popular. We report four interesting cases: Midgut malrotation with volvulus, sigmoid volvulus, biliary rupture of hydatid cyst, and small bowel lipomatosis, where fast imaging employing steady-state acquisition [FIESTA]/constructive interference into steady state [CISS] sequence helped in clinching the diagnosis

Water Quality Criteria for Colored Smokes: Solvent Yellow 33

Science.gov (United States)

1987-11-01

Wr e. a benthic crustacean (e.g, ostracod, isopod, or amphipod): f. an insect (e.g., mayfly, midge, stonefly)- g. a family in a phylum other than...Arthropoda or Chordata (e.g, Annelida or Mollusca): and h, a family in any order of insect or any phylum not represented. 2. Acute-chronic ratios (see...acute toxicity to freshwater acuatic animals should be used: a. Tests with daphnids and other cladocerans should be started with organisms < 24 hr old

Physics calculations for the Clinch River Breeder Reactor

International Nuclear Information System (INIS)

Kalimullah; Kier, P.H.; Hummel, H.H.

1977-06-01

Calculations of distributions of power and sodium void reactivity, unvoided and voided Doppler coefficients and steel and fuel worths have been performed using diffusion theory and first-order perturbation theory for the LWR discharge Pu-fueled CRBR at BOL, the FFTF-grade Pu-fueled CRBR at BOL and for the beginning and end of equilibrium cycle of the LWR-Pu-fueled CRBR. The results of the burnup and breeding ratio calculations performed for obtaining the reactor compositions during the equilibrium cycle are also reported. Effects of sodium and steel contents on the distributions of sodium void reactivity and steel worth have also been studied. Errors and uncertainties in the reactivity coefficients due to cross-sections and the two-dimensional geometric representations of the reactor used in the calculations have also been estimated. Comparisons of the results with those in the CRBR PSAR are also discussed

Student film clinches top prize in film competition

OpenAIRE

Elliott, Jean

2006-01-01

Virginia Tech senior Tim Leaton earned the top prize in the widely acclaimed Film Your Issue (FYI) competition - an eight-week paid internship at Disney Studios in Los Angeles. Leaton's one-minute film, "Orphans in Africa," won the nationwide contest, an initiative to encourage young Americans, age 18 to 26, to engage in social issues and add their voices to the public dialogue.

Daphnid life cycle responses to the insecticide chlorantraniliprole and its transformation products

NARCIS (Netherlands)

Lavtižar, V.; Helmus, R.; Kools, S.A.E.; Dolenc, D; van Gestel, C.A.M.; Trebše, P.; Waaijers, S.L.; Kraak, M.H.S.

2015-01-01

Chlorantraniliprole (CAP) is a newly developed, widely applied insecticide. In the aquatic environment, several transformation products are formed under natural conditions, one by dehydration and others by photoinduced degradation. Data on aquatic ecotoxicity of CAP can mainly be found in

Toxicological benchmarks for screening of potential contaminants of concern for effects on aquatic biota on the Oak Ridge Reservation, Oak Ridge, Tennessee

Energy Technology Data Exchange (ETDEWEB)

Sutter, G.A. II (Oak Ridge National Lab., TN (United States)); Futrell, M.A. (East Tennessee State Univ., Johnson City, TN (United States)); Kerchner, G.A. (Harvard Coll., Cambridge, MA (United States))

1992-09-01

This report presents potential screening benchmarks for protection of aquatic life from contaminants in water. Because there is no guidance for screening benchmarks, a set of alternative benchmarks is presented here. The alternative benchmarks are based on different conceptual approaches to estimating concentrations causing significant effects. For the upper screening benchmark, they are the acute National Ambient Water Quality Criterion (NAWQC) and the acute pesticide advisory value (a concentration that is estimated with 95% confidence not to exceed the unknown acute NAWQC for those chemicals with no NAWQC). The alternative chronic benchmarks are the chronic NAWQC, the chronic pesticide screening value, the lowest chronic values for fish and daphnids, the lowest concentration that produces a response in 20% of fish and daphnids [effective concentration 20 (EC[sub 20])] as established through chronic toxicity tests, the estimated EC[sub 20] for a sensitive species, and the concentration estimated to cause a 20% reduction in the recruit abundance of largemouth bass. It is recommended that ambient chemical concentrations be compared to all of these benchmarks. To the extent that toxicity data are available, this report presents the alternative benchmarks for chemicals that have been detected on the Oak Ridge Reservation. It also presents the data used to calculate the benchmarks, and the sources of the data. It compares the benchmarks and discusses their relative conservatism and utility.

Effect of keratin on heavy metal chelation and toxicity to aquatic organisms

Energy Technology Data Exchange (ETDEWEB)

Coello, W.F.; Khan, M.A.Q. [Univ. of Illinois, Chicago, IL (United States). Dept. of Biological Sciences

1998-12-31

The presence of fresh scales and human hair in water can reduce the toxicity of lead nitrate at and above 6 ppb to fish. This ability is lost on drying and storage, but can be restored if dried hair or scales are treated with a solution of amino acids. The chelation ability of keratin in amino acid-treated scales or hair is retained for months on dry storage. Addition of these hair and/or scales to solutions of lead nitrate, mercuric chloride and a mixture of both, and cupric sulfate reduced the toxicity of these solutions to Daphnia magna and Dreissena polymorpha (zebra mussels). Toxicity of 10 ppm solutions of salts of 27 different metals to daphnids was similarly reduced after filtration through scales or hair. A mixture of a 2 ppb concentration of each of these 27 metals also became nonlethal to daphnids in the presence of, or filtration through, treated scales or hair. 0.25 g of treated hair or scale can be used indefinitely, again and again, to remove the mixture of these 27 metals from their fresh solution in 1 L water if the keratin is frequently rinsed with 0.1% nitric acid to remove the bound metals. The keratin in scales, this, may be the most important ectodermal secretion in absorbing metals from polluted environments and in providing protection against their toxic levels.

Some engineering aspects of the steam generator system for the United States LMFBR demonstration plant

International Nuclear Information System (INIS)

Tippets, F.E.

1975-01-01

This paper describes the main design features of the steam generator system for the Clinch River Breeder Reactor Plant and the engineering approach being employed for some of the critical elements of this system, including in particular the sodium-steam/water boundary, the efforts to have this boundary be of highest integrity, and the system features to safely accommodate any failure of the boundary. (author)

Some engineering aspects of the steam generator system for the United States LMFBR demonstration plant

Energy Technology Data Exchange (ETDEWEB)

Tippets, F E

1975-07-01

This paper describes the main design features of the steam generator system for the Clinch River Breeder Reactor Plant and the engineering approach being employed for some of the critical elements of this system, including in particular the sodium-steam/water boundary, the efforts to have this boundary be of highest integrity, and the system features to safely accommodate any failure of the boundary. (author)

Assessing sediment contamination using six toxicity assays

Directory of Open Access Journals (Sweden)

Allen G. BURTON Jr.

2001-08-01

Full Text Available An evaluation of sediment toxicity at Lake Orta, Italy was conducted to compare a toxicity test battery of 6 assays and to evaluate the extent of sediment contamination at various sediment depths. Lake Orta received excessive loadings of copper and ammonia during the 1900’s until a large remediation effort was conducted in 1989-90 using lime addition. Since that time, the lake has shown signs of a steady recovery of biological communities. The study results showed acute toxicity still exists in sediments at a depth of 5 cm and greater. Assays that detected the highest levels of toxicity were two whole sediment exposures (7 d using Hyalella azteca and Ceriodaphnia dubia. The MicrotoxR assay using pore water was the third most sensitive assay. The Thamnotox, Rototox, Microtox solid phase, and Seed Germination-Root Elongation (pore and solid phase assays showed occasional to no toxicity. Based on similarity of responses and assay sensitivity, the two most useful assays were the C. dubia (or H. azteca and Microtox pore water. These assays were effective at describing sediment toxicity in a weight-of-evidence approach.

Toxicological evaluation of a lake ecosystem contaminated with crude oil

International Nuclear Information System (INIS)

Twigg, D.; Ramey, B.

1995-01-01

Winona Lake on the Daniel Boone National Forest in Powell County, Kentucky, was used from the mid 1950's to 1987 as a water source for water-injection oil drilling and as a brine disposal site. The lake was contaminated with excessive amounts of crude oil. A multi phase investigation was conducted, including chemical analysis of water and sediment, water toxicity tests using a cladoceran, Ceriodaphnia dubia, sediment toxicity tests using an amphipod, Hyalella azteca, and a faunal survey of the communities of the lake and stream both above and below the lake. The sediment was laden with petroleum hydrocarbons (4.1 parts per thousand), while the water showed no contamination. The C dubia test results showed no significant water toxicity. The contaminated sediment adjacent to the dam produced 75% mortality in H. azteca. The faunal survey indicated little or no impact on the upstream and downstream communities but the lake community was highly impacted, especially the benthos. Pollution tolerant Chaoborus sp. were the only organisms collected from sediment samples dredged from the lake. Contamination was limited to the sediment within the lake but the impact on the entire lake community was severe

Assessing contaminant sensitivity of endangered and threatened aquatic species: Part III. Effluent toxicity tests

Science.gov (United States)

Dwyer, F.J.; Hardesty, D.K.; Henke, C.E.; Ingersoll, C.G.; Whites, D.W.; Augspurger, T.; Canfield, T.J.; Mount, D.R.; Mayer, F.L.

2005-01-01

Toxicity tests using standard effluent test procedures described by the U.S. Environmental Protection Agency were conducted with Ceriodaphnia dubia, fathead minnows (Pimephales promelas), and seven threatened and endangered (listed) fish species from four families: (1) Acipenseridae: shortnose sturgeon (Acipenser brevirostrum); (2) Catostomidae; razorback sucker (Xyrauchen texanus); (3) Cyprinidae: bonytail chub (Gila elegans), Cape Fear shiner (Notropis mekistocholas) Colorado pikeminnow (Ptychocheilus lucius), and spotfin chub (Cyprinella monacha); and (4) Poecillidae: Gila topminnow (Poeciliopsis occidentalis). We conducted 7-day survival and growth studies with embryo-larval fathead minnows and analogous exposures using the listed species. Survival and reproduction were also determined with C. dubia. Tests were conducted with carbaryl, ammonia-or a simulated effluent complex mixture of carbaryl, copper, 4-nonylphenol, pentachlorophenol and permethrin at equitoxic proportions. In addition, Cape Fear shiners and spotfin chub were tested using diazinon, copper, and chlorine. Toxicity tests were also conducted with field-collected effluents from domestic or industrial facilities. Bonytail chub and razorback suckers were tested with effluents collected in Arizona whereas effluent samples collected from North Carolina were tested with Cape Fear shiner, spotfin chub, and shortnose sturgeon. The fathead minnow 7-day effluent test was often a reliable estimator of toxic effects to the listed fishes. However, in 21 % of the tests, a listed species was more sensitive than fathead minnows. More sensitive species results varied by test so that usually no species was always more or less sensitive than fathead minnows. Only the Gila topminnow was consistently less sensitive than the fathead minnow. Listed fish species were protected 96% of the time when results for both fathead minnows and C. dubia were considered, thus reinforcing the value of standard whole

Bioassay battery interlaboratory investigation of emerging contaminants in spiked water extracts - Towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring.

Science.gov (United States)

Di Paolo, Carolina; Ottermanns, Richard; Keiter, Steffen; Ait-Aissa, Selim; Bluhm, Kerstin; Brack, Werner; Breitholtz, Magnus; Buchinger, Sebastian; Carere, Mario; Chalon, Carole; Cousin, Xavier; Dulio, Valeria; Escher, Beate I; Hamers, Timo; Hilscherová, Klára; Jarque, Sergio; Jonas, Adam; Maillot-Marechal, Emmanuelle; Marneffe, Yves; Nguyen, Mai Thao; Pandard, Pascal; Schifferli, Andrea; Schulze, Tobias; Seidensticker, Sven; Seiler, Thomas-Benjamin; Tang, Janet; van der Oost, Ron; Vermeirssen, Etienne; Zounková, Radka; Zwart, Nick; Hollert, Henner

2016-11-01

Bioassays are particularly useful tools to link the chemical and ecological assessments in water quality monitoring. Different methods cover a broad range of toxicity mechanisms in diverse organisms, and account for risks posed by non-target compounds and mixtures. Many tests are already applied in chemical and waste assessments, and stakeholders from the science-police interface have recommended their integration in regulatory water quality monitoring. Still, there is a need to address bioassay suitability to evaluate water samples containing emerging pollutants, which are a current priority in water quality monitoring. The presented interlaboratory study (ILS) verified whether a battery of miniaturized bioassays, conducted in 11 different laboratories following their own protocols, would produce comparable results when applied to evaluate blinded samples consisting of a pristine water extract spiked with four emerging pollutants as single chemicals or mixtures, i.e. triclosan, acridine, 17α-ethinylestradiol (EE2) and 3-nitrobenzanthrone (3-NBA). Assays evaluated effects on aquatic organisms from three different trophic levels (algae, daphnids, zebrafish embryos) and mechanism-specific effects using in vitro estrogenicity (ER-Luc, YES) and mutagenicity (Ames fluctuation) assays. The test battery presented complementary sensitivity and specificity to evaluate the different blinded water extract spikes. Aquatic organisms differed in terms of sensitivity to triclosan (algae > daphnids > fish) and acridine (fish > daphnids > algae) spikes, confirming the complementary role of the three taxa for water quality assessment. Estrogenicity and mutagenicity assays identified with high precision the respective mechanism-specific effects of spikes even when non-specific toxicity occurred in mixture. For estrogenicity, although differences were observed between assays and models, EE2 spike relative induction EC 50 values were comparable to the literature, and E2/EE2

Copper regulation and homeostasis of Daphnia magna and Pseudokirchneriella subcapitata: influence of acclimation

International Nuclear Information System (INIS)

Bossuyt, Bart T.A.; Janssen, Colin R.

2005-01-01

This study aimed to evaluate (1) the capacity of the green alga Pseudokirchneriella subcapitata and the waterflea Daphnia magna to regulate copper when exposed to environmentally realistic copper concentrations and (2) the influence of multi-generation acclimation to these copper concentrations on copper bioaccumulation and homeostasis. Based on bioconcentration factors, active copper regulation was observed in algae up to 5 μg Cu L -1 and in daphnids up to 35 μg Cu L -1 . Constant body copper concentrations (13 ± 4 μg Cu g DW -1 ) were observed in algae exposed to 1 through 5 μg Cu L -1 and in daphnids exposed to 1 through 12 μg Cu L -1 . At higher exposure concentrations, there was an increase in internal body copper concentration, while no increase was observed in bioconcentration factors, suggesting the presence of a storage mechanism. At copper concentrations of 100 μg Cu L -1 (P. subcapitata) and 150 μg Cu L -1 (D. magna), the significant increases observed in body copper concentrations and in bioconcentration factors may be related to a failure of this regulation mechanism. For both organisms, internal body copper concentrations lower than 13 μg Cu g DW -1 may result in copper deficiency. For P. subcapitata acclimated to 0.5 and 100 μg Cu L -1 , body copper concentrations ranged (mean ± standard deviation) between 5 ± 2 μg Cu g DW -1 and 1300 ± 197 μg Cu g DW -1 , respectively. For D. magna, this value ranged between 9 ± 2 μg Cu g DW -1 and 175 ± 17 μg Cu g DW -1 for daphnids acclimated to 0.5 and 150 μg Cu L -1 . Multi-generation acclimation to copper concentrations ≥12 μg Cu L -1 resulted in a decrease (up to 40%) in body copper concentrations for both organisms compared to the body copper concentration of the first generation. It can be concluded that there is an indication that P. subcapitata and D. magna can regulate their whole body copper concentration to maintain copper homeostasis within their optimal copper range and

Report on water quality, sediment and water chemistry data for water and sediment samples collected from source areas to Melton Hill and Watts Bar reservoirs

International Nuclear Information System (INIS)

Tomaszewski, T.M.; Bruggink, D.J.; Nunn, D.L.

1995-01-01

Contamination of surface water and sediments in the Clinch River and Watts Bar Reservoir (CR/WBR) system as a result of past and present activities by the US Department of Energy (DOE) on the Oak Ridge Reservation (ORR) and also activities by non-ORR facilities are being studied by the Clinch River Environmental Restoration Program (CR-ERP). Previous studies have documented the presence of heavy metals, organics, and radionuclides in the sediments of reservoirs in the vicinity. In support of the CR-ERP, during the summer of 1991, TVA collected and evaluated water and sediment samples from swimming areas and municipal water intakes on Watts Bar Reservoir, Melton Hill Reservoir and Norris Reservoir, which was considered a source of less-contaminated reference or background data. Despite the numerous studies, until the current work documented by this report, relatively few sediment or water samples had been collected by the CR-ERP in the immediate vicinity of contaminant point sources. This work focused on water and sediment samples taken from points immediately downstream from suspected effluent point sources both on and off the ORR. In August and September, 1994, TVA sampled surface water and sediment at twelve locations in melton Hill and Watts Bar Reservoirs

Statement of Roy F. Pruett, Mayor, City of Oak Ridge, TN

International Nuclear Information System (INIS)

Anon.

1987-01-01

Thank you very much, Mr. Chairman, and Members of the Committee. My name is Roy Pruett, mayor of the city of Oak Ridge, TN, and an executive committee member on the Clinch River MRS task force. It is my pleasure to be here today to testify before this distinguished Committee and present the findings of the Clinch River task force as they relate to subtitle C of the Nuclear Waste Policy Act of 1982, and to the Department of Energy proposal on the Monitored Retrievable Storage facility, the MRS. I represent the city of Oak Ridge, which has been selected for the primary and the first alternative for the siting of an integrated MRS facility for the preparation and packaging of high-level nuclear waste and spent reactor fuel. Of civic and governmental leaders, we concluded that an MRS could safely be built and operated in Oak Ridge. We further concluded, however, that the facility would not be generally perceived as being safe unless the recommendations of the task force were adopted to address concerns and help mitigate impact. Indeed the MRS would not be viewed as a net economic benefit to the site's community, the region, or the State of Tennessee without such appropriate conditions

Deciphering mechanisms of malathion toxicity under pulse exposure of the freshwater cladoceran Daphnia magna

DEFF Research Database (Denmark)

Trác, Ngoc Lâm; Andersen, Ole; Palmqvist, Annemette

2016-01-01

; enzyme activities of acetylcholinesterase (AChE), carboxylesterase (CbE), and glutathione S-transferase (GST); and AChE gene expression. The results showed no difference in survival among equivalent integrated doses. Adverse sublethal effects were driven by exposure concentration rather than pulse...... duration. Specifically, short pulse exposure to a high concentration of malathion resulted in more immobilized daphnids, lower AChE and CbE activities, and a higher transcript level ofAChE gene compared with long pulse exposure to low concentration. The expression of the AChE gene was up...

LMFBR fuel analysis. Task C. Reliability aspects of LMFBRs. Final report, July 1, 1975--September 30, 1976

International Nuclear Information System (INIS)

Apostolakis, G.E.; Cave, L.; Epler, E.P.; Ilberg, D.; Kastenberg, W.E.

1976-11-01

The reliability program which was established for the Clinch River Fast Breeder Reactor Project is reviewed. The review reveals several problems and uncertainties which need to be appropriately addressed by the reliability program. The main problems are stated to be the choice of the reliability criterion, the prospects of attaining high reliabilities of systems and critical structures, the role of external phenomena as well as the verification by tests and the reliability assurance program

Evaluation of the influence of seismic restraint characteristics on breeder reactor piping systems

International Nuclear Information System (INIS)

Mello, R.M.; Pollono, L.P.

1979-01-01

For the Clinch River Breeder Reactor Plant (CRBRP) heat transport system piping within the reactor containment building, dynamic analyses of the piping loops have been performed to study the effect of restraint stiffness on the dynamic behavior of the piping. In addition, analysis and testing of typical CRBRP restraint system components have been performed for the purpose of quantifying and verifying the basic characteristics of the restraints used in the piping system dynamic analysis

Maintenance and repair aspects of the steam generator modules for the United States' LMFBR demonstration plant

Energy Technology Data Exchange (ETDEWEB)

Devlin, R W

1975-07-01

This paper describes the main considerations relating to the field maintenance and repair of the steam generator modules for the Clinch River Breeder Reactor Plant and the development approaches being employed for some of the critical elements of these operations. In particular, the approach to plant chemical cleaning of the waterside of the modules and the approach to recovery from leaks between the water and sodium sides of the modules are discussed. (author)

Exploding the myths about the fast breeder reactor

Energy Technology Data Exchange (ETDEWEB)

Burns, S.

1979-01-01

This paper discusses the facts and figures about the effects of conservation policies, the benefits of the Clinch River Breeder Reactor demonstration plant, the feasibility of nuclear weapons manufacture from reactor-grade plutonium, diversion of plutonium from nuclear plants, radioactive waste disposal, and the toxicity of plutonium. The paper concludes that the U.S. is not proceeding with a high confidence strategy for breeder development because of a variety of false assumptions.

CRBRP design and test results for fuel handling systems, plugs, and seals

International Nuclear Information System (INIS)

Berg, G.E.

1977-01-01

The fuel handling system and reactor rotating plugs for the Clinch River Breeder Reactor Plant (CRBRP) are based primarily on existing technology and, in many respects, follow the concept developed for the Fast Flux Test Facility (FFTF). The equipment and the development programs initiated to verify its performance are described. Test results obtained from the development program, and the extent to which these results verified original design selections, or suggested potential improvements, are discussed

Maintenance and repair aspects of the steam generator modules for the United States' LMFBR demonstration plant

International Nuclear Information System (INIS)

Devlin, R.W.

1975-01-01

This paper describes the main considerations relating to the field maintenance and repair of the steam generator modules for the Clinch River Breeder Reactor Plant and the development approaches being employed for some of the critical elements of these operations. In particular, the approach to plant chemical cleaning of the waterside of the modules and the approach to recovery from leaks between the water and sodium sides of the modules are discussed. (author)

Preliminary physics calculations for the Clinch River Breeder Reactor

International Nuclear Information System (INIS)

Kalimullah.

1975-01-01

Calculations of sodium void, fuel, and clad worths, power distribution, and control rod worths have been carried out for an R-Z model of the CRBR, using diffusion theory and first-order perturbation theory for material worths. The power distribution and control rod worths have also been calculated in two-dimensional triangular mesh geometry. The present results are preliminary because of inaccuracy of the reactor model and the cross sections used, but the final results are not expected to be greatly different. (U.S.)

Evaluating the Effects of the Kingston Fly Ash Release on Fish Reproduction: Spring 2009 - 2010 Studies

Energy Technology Data Exchange (ETDEWEB)

Greeley Jr, Mark Stephen [ORNL; Adams, Marshall [ORNL; McCracken, Kitty [ORNL

2012-05-01

On December 22, 2008, a dike containing fly ash and bottom ash at the Tennessee Valley Authority's (TVA) Kingston Fossil Plant in East Tennessee failed and released a large quantity of ash into the adjacent Emory River. Ash deposits from the spill extended 4 miles upstream of the facility to Emory River mile 6 and downstream to Tennessee River mile 564 ({approx}8.5 miles downstream of the confluence of the Emory River with the Clinch River, and {approx}4 miles downstream of the confluence of the Clinch River with the Tennessee River). A byproduct of coal combustion, fly ash contains a variety of metals and other elements which, at sufficient concentrations and in specific forms, can be harmful to biological systems. The ecological effects of fly ash contamination on exposed fish populations depend on the magnitude and duration of exposure, with the most significant risk considered to come from elevated levels of certain metals in the ash, particularly selenium, on fish reproduction and fish early life stages (Lemly 1993; Besser and others 1996). The ovaries of adult female fish in a lake contaminated by coal ash were reported to have an increased frequency of atretic oocytes (dead or damaged immature eggs) and reductions in the overall numbers of developing oocytes (Sorensen 1988) associated with elevated body burdens of selenium. Larval fish exposed to selenium through maternal transfer of contaminants to developing eggs in either contaminated bodies of water (Lemly 1999) or in experimental laboratory exposures (Woock and others 1987, Jezierska and others 2009) have significantly increased incidences of developmental abnormalities. Contact of fertilized eggs and developing embryos to ash in water and sediments may also pose an additional risk to the early life stages of exposed fish populations through direct uptake of metals and other ash constituents (Jezierska and others 2009). The establishment and maintenance of fish populations is intimately associated

New species of Bakeria (Nematoda; Strongylida; Molineidae), new species of Falcaustra (Nematoda; Ascaridida; Kathlaniidae) and other helminths in Cnemaspis mcguirei (Sauria; Gekkonidae) from Peninsular Malaysia.

Science.gov (United States)

Bursey, Charles R; Goldberg, Stephen R; Grismer, L Lee

2014-10-01

Two new nematode species, Bakeria schadi sp. nov. and Falcaustra malaysiaia sp. nov. from the gastrointestinal tract of McGuire's rock gecko, Cnemaspis mcguirei (Sauria: Gekkonidae) collected in Peninsular Malaysia are described. The two species now assigned to Bakeria are separated on the bases of male bursa type and location of the excretory pore: type II in B. schadi sp. nov. and type I in B. bakeri; location of excretory pore, anterior to nerve ring in B. schadi sp. nov. and posterior to nerve ring in B. bakeri. Falcaustra malaysiaia sp. nov. is most similar to F. chabaudi, F. concinnae, F. condorcanquii, F. barbi, F. dubia, and F. tchadi in that these 7 species possess 1 pseudosucker, 1 median papilla plus 10 pairs caudal papillae, and spicules with lengths between 1 and 2 mm. F. barbi and F. tchadi lack adcloacal papillae; the remaining 5 species possess 1 pair of adcloacal papillae. Falcaustra chabaudi is known from Nearctic salamanders; F. concinnae from Nearctic turtles; F. condorcanquii from Neotropical frogs, F. dubia from Oriental frogs, and F. malaysiaia sp. nov. from Oriental geckos. Two additional species of Nematoda were found, Cosmocerca ornata and Meteterakis singaporensis. Cnemaspis mcguirei represents a new host record for Cosmocerca ornata and Meteterakis singaporensis.

Wary invaders and clever natives: sympatric house geckos show disparate responses to predator scent

OpenAIRE

Adam Cisterne; Eric P. Vanderduys; David A. Pike; Lin Schwarzkopf

2014-01-01

The ability to detect and avoid potential predators can enhance fitness, but also has costs, and thus many animals respond to potential predators either in a general (avoid all potential predators) or threat-sensitive (selectively avoid dangerous predators) manner. We used 2-choice trials to investigate strategies used by globally invasive house geckos (Hemidactylus frenatus) and native Australian house geckos (Gehyra dubia) to avoid chemical cues from potential snake predators (Acanthophis a...

Optimization of intermediate heat exchangers for sodium cooled fast breeder reactors

International Nuclear Information System (INIS)

Barratt, R.O.; Cox, J.; Beith, R.M.V.

1978-01-01

Design evolution of the Intermediate Heat Exchanger for the U.S. Fast Breeder Programme is traced from the initial FFTF through to the Clinch River Designs and anticipated onward Commercial Plant Concepts. Supporting development work is outlined. Technical merits of certain features, such as sine wave tube banks and replaceable tube banks (in-situ), are weighed up against quantified cost advantages with simpler arrangements. Future design trends are outlined which will lead eventually to fully optimised designs. (author)

Aortic atresia with normal sized left ventricle

OpenAIRE

Priya Jagia; Arun Sharma; Saurabh K Gupta; Munish Guleria

2016-01-01

Aortic atresia with an associated ventricular septal defect and adequate sized left ventricle is extremely rare. We present two cases in which an alternate diagnosis was suggested on echocardiography because the hypoplastic aortic trunk was missed due to its small caliber. The final diagnosis was, however, clinched on dual source computed tomography, which not only showed the thin aortic trunk but also clearly depicted the coronary artery origins from the hypoplastic aortic root. To the best ...

TRANSWRAP II: problem definition manual

International Nuclear Information System (INIS)

Knittle, D.E.

1981-02-01

The TRANSWRAP II computer code, written in Fortran IV and described in this Problem Definition Manual, was developed to analytically predict the magnitude of pressure pulses of large scale sodium-wate reactions in LMFBR secondary systems. It is currently being used for the Clinch River Breeder Reactor Program. The code provides the options, flexibility and features necessary to consider any system configuration. The code methodology has been validated with the aid of extensive sodium-water reaction test programs

Rhinoentomophthoromycosis: A rare case report

Directory of Open Access Journals (Sweden)

S Agrawal

2013-01-01

Full Text Available Entomophthoromycosis is chronic granulomatous fungal infection with varied presentation as subcutaneous,mucocutaneous and visceral infections. The majority of the subcutaneous infection caused by entomophthoralean fungi involves Basidiobolus spp, C. coronatus, or C. incongruous. A case of rhinoentomophthoromycosis in an immunocompetent male involving maxillary sinus and nose is presented. The patient was clinically diagnosed as malignancy of nose but microscopy and histopathology of the aspirate clinched the diagnosis. The patient responded to antifungal therepy.

Seismic criteria studies and analyses. Quarterly progress report No. 3. [LMFBR

Energy Technology Data Exchange (ETDEWEB)

1975-01-03

Information is presented concerning the extent to which vibratory motions at the subsurface foundation level might differ from motions at the ground surface and the effects of the various subsurface materials on the overall Clinch River Breeder Reactor site response; seismic analyses of LMFBR type reactors to establish analytical procedures for predicting structure stresses and deformations; and aspects of the current technology regarding the representation of energy losses in nuclear power plants as equivalent viscous damping.

Alignment and operability analysis of a vertical sodium pump

International Nuclear Information System (INIS)

Gupta, V.K.; Fair, C.E.

1981-01-01

With the objective of identifying important alignment features of pumps such as FFTF, HALLAM, EBR II, PNC, PHENIX, and CRBR, alignment of the vertical sodium pump for the Clinch River Breeder Reactor Plant (CRBRP) is investigated. The CRBRP pump includes a flexibly coupled pump shaft and motor shaft, two oil-film tilting-pad hydrodynamic radial bearings in the motor plus a vertical thrust bearing, and two sodium hydrostatic bearings straddling the double-suction centrifugal impeller in the pump

Macrodystrophia lipomatosa: four case reports

Directory of Open Access Journals (Sweden)

Ahmad Ibne

2010-10-01

Full Text Available Abstract Aim Macrodystrophia lipomatosa is a rare cause of gigantism of limb which can be confused with other common causes like congenital lymphedema. It presents usually with loss of function and cosmetic problems. Four cases are described with emphasis on clinical presentation, differential diagnoses, imaging and treatment options. Methods & Results Four patients of macrodystrophia lipomatosa were thoroughly examined and subjected to investigations. Conclusion Besides diligent clinical examination, imaging and histopathology are crucial in clinching the diagnosis.

Macrodystrophia lipomatosa: four case reports

Science.gov (United States)

2010-01-01

Aim Macrodystrophia lipomatosa is a rare cause of gigantism of limb which can be confused with other common causes like congenital lymphedema. It presents usually with loss of function and cosmetic problems. Four cases are described with emphasis on clinical presentation, differential diagnoses, imaging and treatment options. Methods & Results Four patients of macrodystrophia lipomatosa were thoroughly examined and subjected to investigations. Conclusion Besides diligent clinical examination, imaging and histopathology are crucial in clinching the diagnosis. PMID:20969776

Florid reactive lymphoid hyperplasia of terminal ileum

OpenAIRE

Kanakala, Venkatesh; Birch, Peter; Kasaraneni, Ramesh

2010-01-01

Florid lymphoid hyperplasia in the terminal ileum can present to surgeons as an acute abdominal pain. Only few cases were reported in the literature. Our case illustrates that a rare case of florid lymphoid hyperplasia can present to surgeons as acute appendicitis. During the operation the gross appearance may mimic Crohn’s disease. A limited resection is sufficient to clinch the diagnosis of florid lymphoid hyperplasia / Crohn’s disease. In florid lymphoid hyperplasia limited resection may b...

n vivo retention of ingested Au NPs by Daphnia magna: No evidence for trans-epithelial alimentary uptake

Science.gov (United States)

Khan, Farhan R.; Kennaway, Gabrielle M.; Croteau, Marie-Noële; Dybowska, Agnieszka; Smith, Brian D.; Nogueira, António J.A.; Rainbow, Philip S.; Luoma, Samuel N.; Valsami-Jones, Eugenia

2014-01-01

In vivo studies with Daphnia magna remain inconclusive as to whether engineered nanoparticles (NPs) are internalized into tissues after ingestion. Here we used a three-pronged approach to study the in vivo retention and efflux kinetics of 20 nm citrate stabilized Au NPs ingested by this key aquatic species. Daphnids were exposed to suspended particles (600 μg L−1) for 5 h after which they were depurated for 24 h in clean water containing algae. Light microscopy was used to follow the passage of Au NPs through the gastrointestinal tract, Au body burdens were determined by ICP-MS (inductively coupled plasma mass spectrometry), and transmission electron microscopy (TEM) was used to examine the presence and distribution of Au NPs in tissues. Results revealed that the elimination of Au NPs was bi-phasic. The fast elimination phase lasted −1 (±SE) which accounted for ∼75% of the ingested Au. The remaining ∼25% of the ingested Au NPs was eliminated at a 100-fold slower rate. TEM analysis revealed that Au NPs in the midgut were in close proximity to the peritrophic membrane after 1 and 24 h of depuration. There were no observations of Au NP uptake at the microvilli. Thus, although Au NPs were retained in the gut lumen, there was no observable internalization into the gut epithelial cells. Similar to carbon nanotubes and CuO NPs, our findings indicate that in daphnids the in vivo retention of Au NPs does not necessarily result in their internalization.

Comparative ovarian microarray analysis of juvenile hormone-responsive genes in water flea Daphnia magna: potential targets for toxicity.

Science.gov (United States)

Toyota, Kenji; Williams, Timothy D; Sato, Tomomi; Tatarazako, Norihisa; Iguchi, Taisen

2017-03-01

The freshwater zooplankton Daphnia magna has been extensively employed in chemical toxicity tests such as OECD Test Guidelines 202 and 211. Previously, it has been demonstrated that the treatment of juvenile hormones (JHs) or their analogues to female daphnids can induce male offspring production. Based on this finding, a rapid screening method for detection of chemicals with JH-activity was recently developed using adult D. magna. This screening system determines whether a chemical has JH-activity by investigating the male offspring inducibility. Although this is an efficient high-throughput short-term screening system, much remains to be discovered about JH-responsive pathways in the ovary, and whether different JH-activators act via the same mechanism. JH-responsive genes in the ovary including developing oocytes are still largely undescribed. Here, we conducted comparative microarray analyses using ovaries from Daphnia magna treated with fenoxycarb (Fx; artificial JH agonist) or methyl farnesoate (MF; a putative innate JH in daphnids) to elucidate responses to JH agonists in the ovary, including developing oocytes, at a JH-sensitive period for male sex determination. We demonstrate that induction of hemoglobin genes is a well-conserved response to JH even in the ovary, and a potential adverse effect of JH agonist is suppression of vitellogenin gene expression, that might cause reduction of offspring number. This is the first report demonstrating different transcriptomics profiles from MF and an artificial JH agonist in D. magna ovary, improving understanding the tissue-specific mode-of-action of JH. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

RIBE at an inter-organismic level: A study on genotoxic effects in Daphnia magna exposed to waterborne uranium and a uranium mine effluent.

Science.gov (United States)

Reis, P; Lourenço, J; Carvalho, F P; Oliveira, J; Malta, M; Mendo, S; Pereira, R

2018-05-01

The induction of RIBE (Radiation Induced Bystander Effect) is a non-target effect of low radiation doses that has already been verified at an inter-organismic level in fish and small mammals. Although the theoretical impact in the field of environmental risk assessment (ERA) is possible, there is a gap of knowledge regarding this phenomenon in invertebrate groups and following environmentally relevant exposures. To understand if RIBE should be considered for ERA of radionuclide-rich wastewaters, we exposed Daphnia magna (uranium mine effluent for 48 h, and to a matching dose of waterborne uranium (55.3 μg L -1 ). Then the exposed organisms were placed (24 and 48 h) in a clean medium together with non-exposed neonates. The DNA damage observed for the non-exposed organisms was statistically significant after the 24 h cohabitation for both uranium (neonates p = 0.002; 5 d-old daphnids p = uranium mine effluent exposure (only for neonates p = 0.042). After 48 h cohabitation significant results were obtained only for uranium exposure (neonates p = 0.017; 5 d-old daphnids p = 0.013). Although there may be some variability associated to age and exposure duration, the significant DNA damage detected in non-exposed organisms clearly reveals the occurrence of RIBE in D. magna. The data obtained and here presented are a valuable contribution for the discussion about the relevance of RIBE for environmental risk assessment. Copyright © 2018 Elsevier B.V. All rights reserved.

Short-term exposure with high concentrations of pristine microplastic particles leads to immobilisation of Daphnia magna.

Science.gov (United States)

Rehse, Saskia; Kloas, Werner; Zarfl, Christiane

2016-06-01

Recent studies revealed that freshwaters are not only polluted by chemicals, but also by persistent synthetic material like microplastics (plastic particles pollutants or release additives. Although there is rising concern about the pollution of freshwaters by microplastics, knowledge about their potential effects on organisms is limited. For a better understanding of their risks, it is crucial to unravel which characteristics influence their effects on organisms. Analysing effects by the mere particles is the first step before including more complex interactions e.g. with associated chemicals. The aim of this study was to analyse potential physical effects of microplastics on one representative organism for limnic zooplankton (Daphnia magna). We investigated whether microplastics can be ingested and whether their presence causes adverse effects after short-term exposure. Daphnids were exposed for up to 96 h to 1-μm and 100-μm polyethylene particles at concentrations between 12.5 and 400 mg L(-1). Ingestion of 1-μm particles led to immobilisation increasing with dose and time with an EC50 of 57.43 mg L(-1) after 96 h. 100-μm particles that could not be ingested by the daphnids had no observable effects. These results underline that, considering high concentrations, microplastic particles can already induce adverse effects in limnic zooplankton. Although it needs to be clarified if these concentrations can be found in the environment these results are a basis for future impact analysis, especially in combination with associated chemicals. Copyright © 2016 Elsevier Ltd. All rights reserved.

Can mixtures of cyanotoxins represent a risk to the zooplankton? The case study of Daphnia magna Straus exposed to hepatotoxic and neurotoxic cyanobacterial extracts.

Science.gov (United States)

Freitas, Emanuela Cristina; Pinheiro, Carlos; Rocha, Odete; Loureiro, Susana

2014-01-01

Worldwide, cyanobacterial blooms have been increasing in intensity and frequency, with toxic cyanobacteria sometimes dominant throughout the year in many freshwater bodies. Since the coexistence of more than one type of cyanotoxins in freshwater environments is a common phenomenon, studies on the joint effects of these toxins would be very useful. In this study, the single and combined effects of two cyanotoxins with different modes of action (hepatotoxic and neurotoxic) on the survival (lethal exposure) and feeding (sublethal exposure) of the cladoceran Daphnia magna were investigated. With the single exposures, it was observed that both the survival and feeding activity of the daphnids were impaired by the hepatotoxic and neurotoxic extracts at environmentally relevant concentrations. In the combined exposures, both survival and feeding rate endpoints showed a good fit to the independent action model. For the acute assay and 24h exposure period in the feeding inhibition test, there was no interaction between components of the hepatotoxic and neurotoxic extracts, although a slight tendency to a synergistic deviation could be seen in the feeding rates. On the other hand, for the 4h post-exposure period, a synergistic deviation was found in feeding rates at all mixture concentrations tested. Hence, the combined exposure of hepatotoxins and neurotoxins should also be taken into account in risk assessments of freshwater bodies, since the mixture of these toxins can result in more severe post-exposure effects on the feeding of daphnids than the sum of those expected for single exposures. Copyright © 2013 Elsevier B.V. All rights reserved.

Epidemiology of Muay Thai fight-related injuries

OpenAIRE

Strotmeyer, Stephen; Coben, Jeffrey H.; Fabio, Anthony; Songer, Thomas; Brooks, Maria

2016-01-01

Background Muay Thai is a combat sport of Thailand that uses stand-up striking along with various clinching techniques. Currently, little is known about the injuries and risk factors for injuries among Muay Thai fighters. Gaining more insight into the nature and frequency of injury in this sport provides part of the overall sports injury picture, within the larger burden of injury as a public health issue. Generating this information is a critical first step toward the broader goal of improvi...

CRBRP structural and thermal margin beyond the design base

International Nuclear Information System (INIS)

Strawbridge, L.E.

1979-01-01

Prudent margins beyond the design base have been included in the design of Clinch River Breeder Reactor Plant to further reduce the risk to the public from highly improbable occurrences. These margins include Structural Margin Beyond the Design Base to address the energetics aspects and Thermal Margin Beyond the Design Base to address the longer term thermal and radiological consequences. The assessments that led to the specification of these margins are described, along with the experimental support for those assessments. 8 refs

Penerapan Teori Permainan dalam Analisa Persaingan Pasar Produk Sepeda Motor Honda dengan Yamaha di Universitas Sumatera Utara

OpenAIRE

Tambunan, Erbin

2015-01-01

Automotive industry of Indonesia was enlivened by a choice variety of products especially of the motorcycle. Many choices with the motorcycle products resulted in rivalry, in which the object is clinched the market with most sales. Market, tough competition in demanding companies motorcycle to capable of being decided on the optimum strategy for their products. To the research is aimed to get strategy optimum for the company. Case study taken is the product honda and yamaha which are shared o...

Design and development of the CRBRP ex-vessel transfer machine

International Nuclear Information System (INIS)

Jones, C.E. Jr.

1977-01-01

The Reactor Refueling System (RRS) for the Clinch River Breeder Reactor Project (CRBRP) uses the Ex-Vessel Transfer Machine (EVTM) for transferring core assemblies outside the reactor vessel. The design of the Ex-Vessel Transfer Machine (EVTM) and its gantry-trolly for the CRBRP is discussed. The development tests required for the design are presented, in conjunction with the impact of the test results on the design. The impact of the increased seismic requirements on the design are also presented

Transfers and transformations of zinc in flow-through wetland microcosms.

Science.gov (United States)

Gillespie, W B; Hawkins, W B; Rodgers, J H; Cano, M L; Dorn, P B

1999-06-01

Two microcosm-scale wetlands (570-liter containers) were integratively designed and constructed to investigate transfers and transformations of zinc associated with an aqueous matrix, and to provide future design parameters for pilot-scale constructed wetlands. The fundamental design of these wetland microcosms was based on biogeochemical principles regulating fate and transformations of zinc (pH, redox, etc.). Each wetland consisted of a 45-cm hydrosoil depth inundated with 25 cm of water, and planted with Scirpus californicus. Zinc ( approximately 2 mg/liter) as ZnCl2 was amended to each wetland for 62 days. Individual wetland hydraulic retention times (HRT) were approximately 24 h. Total recoverable zinc was measured daily in microcosm inflow and outflows, and zinc concentrations in hydrosoil and S. californicus tissue were measured pre- and post-treatment. Ceriodaphnia dubia and Pimephales promelas7-day aqueous toxicity tests were performed on wetland inflows and outflows, and Hyalella azteca whole sediment toxicity tests (10-day) were performed pre- and post-treatment. Approximately 75% of total recoverable zinc was transferred from the water column. Toxicity decreased from inflow to outflow based on 7-day C. dubia tests, and survival of H. azteca in hydrosoil was >80%. Data illustrate the ability of integratively designed wetlands to transfer and sequester zinc from the water column while concomitantly decreasing associated toxicity. Copyright 1999 Academic Press.

Acute aquatic toxicity of tire and road wear particles to alga, daphnid, and fish.

Science.gov (United States)

Marwood, Christopher; McAtee, Britt; Kreider, Marisa; Ogle, R Scott; Finley, Brent; Sweet, Len; Panko, Julie

2011-11-01

Previous studies have indicated that tire tread particles are toxic to aquatic species, but few studies have evaluated the toxicity of such particles using sediment, the likely reservoir of tire wear particles in the environment. In this study, the acute toxicity of tire and road wear particles (TRWP) was assessed in Pseudokirchneriella subcapita, Daphnia magna, and Pimephales promelas using a sediment elutriate (100, 500, 1000 or 10000 mg/l TRWP). Under standard test temperature conditions, no concentration response was observed and EC/LC(50) values were greater than 10,000 mg/l. Additional tests using D. magna were performed both with and without sediment in elutriates collected under heated conditions designed to promote the release of chemicals from the rubber matrix to understand what environmental factors may influence the toxicity of TRWP. Toxicity was only observed for elutriates generated from TRWP leached under high-temperature conditions and the lowest EC/LC(50) value was 5,000 mg/l. In an effort to identify potential toxic chemical constituent(s) in the heated leachates, toxicity identification evaluation (TIE) studies and chemical analysis of the leachate were conducted. The TIE coupled with chemical analysis (liquid chromatography/mass spectrometry/mass spectrometry [LC/MS/MS] and inductively coupled plasma/mass spectrometry [ICP/MS]) of the leachate identified zinc and aniline as candidate toxicants. However, based on the high EC/LC(50) values and the limited conditions under which toxicity was observed, TRWP should be considered a low risk to aquatic ecosystems under acute exposure scenarios.

Avaliação ecotoxicológica de efluentes de celulose branqueada de eucalipto ao longo do tratamento biológico

OpenAIRE

Martins, Daniel Von Rondon

2008-01-01

O presente estudo teve como objetivo avaliar a toxicidade de efluentes de celulose branqueada de eucalipto. Inicialmente foi feita uma caracterização físicoquímica das amostras, em diferentes pontos ao longo do tratamento, e posteriormente para estes mesmos pontos foi avaliada a toxicidade com quatro organismos-teste: para toxicidade aguda: a) Vibrio fischeri (sistema Microtox®) e b) Daphnia similis; para toxicidade crônica: c) Ceriodaphnia dubia e d) Pseudokirchneriella subcapitata. Para a r...

SIMMER Program: its accomplishments

International Nuclear Information System (INIS)

Smith, L.L.; Bell, C.R.; Bohl, W.R.; Luck, L.B.; Wehner, T.R.; DeVault, G.P.; Parker, F.R.

1985-01-01

SIMMER's mechanistic framework has contributed to substantial progress in understanding severe LMFBR accidents, the important accident processes, the process environments, and the need for consistency in assessing the total accident sequence. A comprehensive program of model and code development, experiment analyses, separate-effects studies, and whole-core accident calculations has resolved issues in transition-phase, behavior and showed convincingly that Clinch River Breeder Reactor accident energetics were within design requirements. The current international program continues progress toward improving our understanding of severe accidents in LMFBRs. 17 refs

Secure Automated Fabrication: an overview of remote breeder fuel fabrication

International Nuclear Information System (INIS)

Nyman, D.H.; Graham, R.A.

1983-10-01

The Secure Automated Fabrication (SAF) line is an automated, remotely controlled breeder fuel pin fabrication process which is to be installed in the Fuels and Materials Examination Facility (FMEF). The FMEF is presently under construction at Hanford and is scheduled for completion in 1984. The SAF line is scheduled for startup in 1987 and will produce mixed uranium-plutonium fuel pins for the Fast Flux Test Facility (FFTF) and the Clinch River Breeder Reactor Plant (CRBRP). The fabrication line and support systems are described

Breeder Spent Fuel Handling (BSFH) cask study for FY83. Final report

International Nuclear Information System (INIS)

Diggs, J.M.

1985-01-01

This report documents a study conducted to investigate the applicability of existing LWR casks to shipment of long-cooled LMFBR fuel from the Clinch River Breeder Reactor Plant (CRBRP) to the Breeder Reprocessing Engineering Test (BRET) Facility. This study considered a base case of physical constraints of plants and casks, handling capabilities of plants, through-put requirements, shielding requirements due to transportation regulation, and heat transfer capabilities of the cask designs. Each cask design was measured relative to the base case. 15 references, 4 figures, 6 tables

Limnological and ecotoxicological studies in the cascade of reservoirs in the Tietê river (São Paulo, Brazil Estudos limnológicos e ecotoxicológicos na cascata de reservatórios do rio Tietê (São Paulo, Brasil

Directory of Open Access Journals (Sweden)

S. Rodgher

2005-11-01

Full Text Available An evaluation was made of the quality of samples of water and sediment collected from a series of reservoirs in the Tietê River (SP, based on limnological and ecotoxicological analyses. The samples were collected during two periods (Feb and Jul 2000 from 15 sampling stations. Acute toxicity bioassays were performed using the test organism Daphnia similis, while chronic bioassays were carried out withCeriodaphnia dubia and Danio rerio larvae. The water samples were analyzed for total nutrients, total suspended matter and total cadmium, chromium, copper and zinc concentrations, while the sediment samples were examined for organic matter, granulometry and potentially bioavailable metals (cadmium, chromium, copper and zinc. The results obtained for the limnological variable, revealed differences in the water quality, with high contribution of nutrients and metals for Tietê and Piracicaba rivers, besides the incorporation and sedimentation, consequently causing a reduction of materials in Barra Bonita reservoir, thus promoting the improvement of the water quality in the other reservoirs. The toxicity bioassays revealed acute toxicity for Daphnia similis only in the reservoirs located below Barra Bonita dam. On the other hand, chronic toxicity for Ceriodaphnia dubia and acute for Danio rerio showed a different pattern, decreasing in magnitude from Barra Bonita to Três Irmãos, demonstrating an environmental degradation gradient in the reservoirs.O presente trabalho visou avaliar a qualidade de amostras de água e sedimento dos reservatórios em cascata do rio Tietê (SP através de análises limnológicas e ecotoxicológicas. Foram realizadas coletas de água e sedimento em dois períodos (fevereiro e julho de 2000 e em 15 estações de amostragem. Foram realizados bioensaios de toxicidade aguda para Daphnia similis, de toxicidade crônica para Ceriodaphnia dubia e para larvas pós-eclodidas de Danio rerio. Análises de nutrientes totais, material

Hairy roots induction and artemisinin analysis in Artemisia dubia ...

African Journals Online (AJOL)

STORAGESEVER

2008-09-17

Sep 17, 2008 ... When transformed roots were cultured in liquid medium, highest root fresh weight as well as .... To make the final volume 4 ml,. 400 µL of ..... and bubble column reactors in the in vitro production of artemisinin. Plant Cell Rep.

Biological Monitoring and Abatement Program for the Oak Ridge K-25 Site

International Nuclear Information System (INIS)

Kszos, L.A.; Adams, S.M.; Ashwood, T.L.; Blaylock, B.G.; Greeley, M.S.; Loar, J.M.; Peterson, M.J.; Ryon, M.G.; Smith, J.G.; Southworth, G.R.; Hinzman, R.L.; Shoemaker, B.A.

1993-04-01

A proposed Biological Monitoring and Abatement Program (BMAP) for the Oak Ridge K-25 Site was prepared in December 1992 as required by the renewed National Pollutant Discharge Elimination System permit that was issued on October 1, 1992. The proposed BMAP is based on results of biological monitoring conducted from 1986 to 1992 and discussions held on November 12, 1992, between staff of Martin Marietta Energy Systems, Inc. (Oak Ridge National Laboratory and the K-25 Site), and the Tennessee Department of Environment and Conservation, Department of Energy Oversight Division. The proposed BMAP consists of four tasks that reflect different but complementary approaches to evaluating the effects of K-25 Site effluents on the ecological integrity of Mitchell Branch, Poplar Creek, and the Poplar Creek embayment of the Clinch River. These tasks include (1) ambient toxicity monitoring, (2) bioaccumulation monitoring, (3) assessment of fish health, and (4) instream monitoring of biological communities. This overall BMAP plan combines established protocols with current biological monitoring techniques to assess environmental compliance and quantify ecological recovery. The BMAP will also determine whether the effluent limits established for the K-25 Site protect the designated use of the receiving streams (Mitchell Branch, Poplar Creek, and Clinch River) for growth and propagation of fish and other aquatic life. Results obtained from this biological monitoring program will also be used to document the ecological effects (and effectiveness) of remedial actions

Biological Monitoring and Abatement Program for the Oak Ridge K-25 Site

Energy Technology Data Exchange (ETDEWEB)

Kszos, L.A.; Adams, S.M.; Ashwood, T.L.; Blaylock, B.G.; Greeley, M.S.; Loar, J.M.; Peterson, M.J.; Ryon, M.G.; Smith, J.G.; Southworth, G.R. (Oak Ridge National Lab., TN (United States)); Hinzman, R.L. (Oak Ridge Research Inst., TN (United States)); Shoemaker, B.A. (Oak Ridge K-25 Site, TN (United States))

1993-04-01

A proposed Biological Monitoring and Abatement Program (BMAP) for the Oak Ridge K-25 Site was prepared in December 1992 as required by the renewed National Pollutant Discharge Elimination System permit that was issued on October 1, 1992. The proposed BMAP is based on results of biological monitoring conducted from 1986 to 1992 and discussions held on November 12, 1992, between staff of Martin Marietta Energy Systems, Inc. (Oak Ridge National Laboratory and the K-25 Site), and the Tennessee Department of Environment and Conservation, Department of Energy Oversight Division. The proposed BMAP consists of four tasks that reflect different but complementary approaches to evaluating the effects of K-25 Site effluents on the ecological integrity of Mitchell Branch, Poplar Creek, and the Poplar Creek embayment of the Clinch River. These tasks include (1) ambient toxicity monitoring, (2) bioaccumulation monitoring, (3) assessment of fish health, and (4) instream monitoring of biological communities. This overall BMAP plan combines established protocols with current biological monitoring techniques to assess environmental compliance and quantify ecological recovery. The BMAP will also determine whether the effluent limits established for the K-25 Site protect the designated use of the receiving streams (Mitchell Branch, Poplar Creek, and Clinch River) for growth and propagation of fish and other aquatic life. Results obtained from this biological monitoring program will also be used to document the ecological effects (and effectiveness) of remedial actions.

Licensing decisions and safety research related to LMFBR accidents

International Nuclear Information System (INIS)

Denise, R.P.; Speis, T.P.; Kelber, C.N.; Curtis, R.T.

1977-01-01

The licensing approach which ensures adequate protection of the public health and safety against serious accidents is described. This paper describes the role of core melt and core disruptive accidents in the design, safety research, and licensing processes, using the Clinch River Breeder Reactor (CRBR) as a focal point. Major design attention is placed on the prevention of these accidents so that the probability of core melt accidents is reduced to a sufficiently low level that they are not treated as design basis accidents. Additional requirements are placed upon the design to further reduce residual risk. This licensing process is supported by a confirmatory research program designed to provide an independent basis for licensing judgements. It has as a goal the resolution of generic safety issues prior to the establishment of a commercial LMFBR industry. The program includes accident analysis, experiments in materials interactions, aerosol transport and system integrity and planning for new safety test facilities. The problems are approached in a multi-disciplinary functional manner that identifies key safety issues and centralizes efforts to resolve them. The near term objectives of the program support the licensing of the Clinch River Breeder Reactor (CRBR) and the proposed Prototype Large Breeder Reactor (PLBR). The long term objectives of the program support the licensing of commercial LMFBRs during the late 1980's and beyond. This safety research is designed to provide an independent basis for the licensing judgements which must be made by the Nuclear Regulatory Commission

Species differences in contaminants in fish on and adjacent to the Oak Ridge Reservation, Tennessee

International Nuclear Information System (INIS)

Burger, Joanna; Campbell, K.R.

2004-01-01

Risks to humans and other organisms from consuming fish have become a national concern in the USA. In this paper, we examine the concentrations of 137 Cs, arsenic, beryllium, cadmium, lead, mercury, and selenium in three species of fish from two river reaches adjacent to the US Department of Energy's Oak Ridge Reservation in Tennessee. We were interested in whether there were species and locational differences in radiocesium and metal concentrations and whether concentrations were sufficiently high to pose a potential health risk to humans or other receptors. Striped bass (Morone saxatilis) were significantly larger than white bass (M. chrysops), and crappie (Pomoxis spp.) were the smallest fish. Lead was significantly lower in striped bass, mercury was significantly higher in striped bass, and selenium was significantly higher in white bass compared to the other species. There were no other species differences in contaminants. White bass, the only species that was sufficiently abundant for a comparison, had significantly higher concentrations of cadmium, lead, and selenium in fillets from the Clinch River and significantly higher concentrations of mercury in fillets from Poplar Creek. The low concentrations of most contaminants in fish from the Clinch River do not appear to present a risk to humans or other consumers, although mercury concentrations in striped bass ranged as high as 0.79 ppm, well above the 0.5-ppm action level for human consumption of some US states

On the suitability of fiberglass reinforced polyester as building material for mesocosms.

Science.gov (United States)

Berghahn, R; Brandsch, J; Piringer, O; Pluta, H J; Winkler, T

1999-07-01

Gel- and topcoat surface layers on fiberglass [glass-reinforced plastic (GRP)] made of unsaturated resin based on isophthalic acid polyester and neopentyl glycol (ISO-NPG) were tested for leaching, ecotoxicity of water eluates, and abrasion by river sediments at a current speed of 0.5 m * s-1. Leaching from topcoat tempered at low temperature was significant, whereas it was negligible from highly tempered gelcoat. Water eluates from both gel-and topcoat were nontoxic in routinely employed biotests (bacteria, algae, daphnids). No abrasion by river sediments was detectable. Based on these results, GRP with gelcoat made of ISO-NPG is considered a suitable building material for mesocosms. Copyright 1999 Academic Press.

Primary intracranial leiomyoma in renal transplant recipient

Directory of Open Access Journals (Sweden)

Upasana Patel

2017-01-01

Full Text Available Leiomyoma, the benign tumor of smooth muscle cell origin, is commonly seen in genitourinary and gastrointestinal tracts. Primary intracranial leiomyoma, however, is extremely rare occurrence. We hereby report a case of Epstein-Barr negative primary intracranial leiomyoma in a middle-aged renal transplant recipient, which mimicked left frontal parasagittal meningioma on neuroimaging. The tumor was completely excised and diagnosis of leiomyoma was clinched on pathological analysis with immunohistochemistry. The patient improved after tumor removal, and no evidence of tumor recurrence was noted on follow-up study after 10 months postsurgically.

PRISM reactor system design and analysis of postulated unscrammed events

International Nuclear Information System (INIS)

Van Tuyle, G.J.; Slovik, G.C.

1991-01-01

Key safety characteristics of the PRISM reactor system include the passive reactor shutdown characteristic and the passive shutdown heat removal system, RVACS. While these characteristics are simple in principle, the physical processes are fairly complex, particularly for the passive reactor shutdown. It has been possible to adapt independent safety analysis codes originally developed for the Clinch River Breeder Reactor review, although some limitations remain. In this paper, the analyses of postulated unscrammed events are discussed, along with limitations in the predictive capabilities and plans to correct the limitations in the near future. (author)

CRBRP flow induced vibration program

Energy Technology Data Exchange (ETDEWEB)

Novendstern, E H [Westinghouse Advanced Reactor Division, Madison, PA (United States); Grochowski, F A; Yang, T M [General Electric Co., Fast Breeder Reactor Department, Sunnyvale, CA (United States); Ryan, J A; Mulcahy, T M

1977-12-01

The program to assure the structural adequacy of Clinch River Breeder Reactor (CRBRP) components during its planned 30 years of operation is described. The program includes (1) an assessment of reactor components relative to their susceptibility to FIV, (2) designing to minimize component excitation due to Fluid induced vibrations (FIV), (3) scale model tests to measure structural response during simulated operating conditions and (4) preoperational tests. An overview of the CRBRP test program is described. Additionally, details of scale model testing of reactor internals and the steam generator is described in more detail. (author)

Mechanical joining of materials with limited ductility: Analysis of process-induced defects

Science.gov (United States)

Jäckel, M.; Coppieters, S.; Hofmann, M.; Vandermeiren, N.; Landgrebe, D.; Debruyne, D.; Wallmersberger, T.; Faes, K.

2017-10-01

The paper shows experimental and numerical analyses of the clinching process of 6xxx series aluminum sheets in T6 condition and the self-pierce riveting process of an aluminum die casting. In the experimental investigations the damage behavior of the materials when using different tool parameters is analyzed. The focus of the numerical investigations is the damage prediction by a comparison of different damage criteria. Moreover, strength-and fatigue tests were carried out to investigate the influence of the joining process-induced damages on the strength properties of the joints.

Monitored retrievable storage status

International Nuclear Information System (INIS)

Carlson, J.H.

1985-01-01

The Department of Energy (DOE) is preparing a proposal for Congressional consideration of the inclusion of a monitored retrievable storage (MRS) facility as part of the Federal Nuclear Waste Management System. The DOE plans to submit the proposal package to the Congress by January 15, 1986. The proposed preferred location of the MRS facility is the former Clinch River Breeder site in the state of Tennessee. If the Congress approves implementation of the MRS proposal, it is estimated that the facility could be licensed and operational in ten years

Efficiency of removing nitrogen and phosphorous and effects on extracellular enzyme activity in water body by five hydrophytes%5种水生植物的脱氮除磷效果及其对水体胞外酶活的影响

Institute of Scientific and Technical Information of China (English)

汪文强; 王子芳; 高明

2016-01-01

To investigate the role of hydrophytes in the purification of eutrophic water bodies,Trapa quadrispinosa Roxb.,Eichhornia crassipes,Hydrocharis dubia (Bl.) Backer,Ipomoea aquatica Forsk,and Lemna minor L.were planted in treated water to compare their effects on purification and their influence on extracellular enzyme activity.The results showed that all hydrophytes performed well during the purification process.For total nitrogen (TN),the order of removal efficiency was Eichhornia crassipes (81.6％) ＞ Ipomoea aquatica Forsk (72.2％) ＞ Hydrocharis dubia (Bl.) Backer (68.4％) ＞ Trapa quadrispinosa Roxb.(65.8％) ＞ Lemna minor L.(60.2％),and for total phosphorous (TP) removal,the order was Eichhornia crassipes (95.0％) ＞ Ipomoea aquatica Forsk (89.7％) ＞ Hydrocharis dubia (Bl.) Backer (78.4％) ＞ Trapa quadrispinosa Roxb.(77.3％) ＞ Lemna minor L.(70.5％).All five hydrophytes could enhance the activity of extracellular enzymes,and the treatment involving E.crassipes showed the maximum range and peak urease activity (UA) and alkaline phosphatase activity (APA).The range and peak UA were 19.70 μg · mL-1 and 10.33 μg · mL-1,respectively,and for APA,they were 8.12 μg · mL-1 and 5.95 μg · mL-1.The range and peak UA and APA for Ipomoea aquatica Forsk were the next highest.UA and APA were significantly negatively correlated with the concentrations of both NH4-N and dissolved active phosphorus (SRP),and the correlations were between-0.853 and-0.992 and -0.813 and-0.994,respectively.Eichhornia crassipes and Ipomoea aquatica Forsk may work better than Trapa quadrispinosa Roxb,Hydrocharis dubia (Bl.) Backer and Lemna minor L.,and have potential for application in the purification of eutrophic water bodies.%利用静态小区实验研究了菱角(Trapa quadrispinosa Roxb.)、凤眼莲(Eichhornia crassipes.)、水鳖(Hydrocharis dubia (Bl.) Backer)、空心菜(Ipomoea aquatica Forsk)和青萍(Lemna minor L.)5�

Evaluación ecotoxicológica de sedimentos en una zona del Río Uruguay, con puntos finales indicadores de toxicidad aguda, sub-letal, crónica, reproductiva y teratogénica

Directory of Open Access Journals (Sweden)

Diana Míguez Carames

2011-05-01

Full Text Available El objetivo de este trabajo fue caracterizar la toxicidad de los sedimentos de una zona del Río Uruguay, aplicando una batería de bioensayos. Las muestras provinieron de: una zona de influencia de descargas domésticas y efluentes de una planta de celulosa (Fray Bentos, una ciudad turística aguas abajo (Las Cañas y otra aguas arriba (Nuevo Berlín, sin influencia directa de dichos vertidos. Los bioensayos aplicados en elutriados fueron: de toxicidad con Photobacterium leiognathi, de reproducción con Ceriodaphnia dubia, de teratogenicidad y letalidad con Pimephales promelas y el ensayo sub-letal en sedimento completo con Hyalella curvispina. Para evaluar la biodisponibilidad y las rutas de toxicidad, se midió el fraccionamiento del 2,4,5-triclorofenol, con resultados: 63% disuelto, 27% adsorbido sobre partículas y el resto sedimentado.Se observó toxicidad por orgánicos y metales con P. leioghnati para todos los sitios. La frecuencia de malformaciones vertebralesde P. promelas fue de 3.3% en Fray Bentos, pero C. dubia sólo mostró efectos en Nuevo Berlín y Las Cañas. Los efectos con H. curvispina se evidenciaron en todas las muestras. Las rutas de toxicidad incluirían la ingestión de contaminantes disueltos y particulados asociados a arcillas y limo. El vínculo causa-efecto para la toxicidad se continuará investigando.

Pesticide Toxicity Index: a tool for assessing potential toxicity of pesticide mixtures to freshwater aquatic organisms

Science.gov (United States)

Nowell, Lisa H.; Norman, Julia E.; Moran, Patrick W.; Martin, Jeffrey D.; Stone, Wesley W.

2014-01-01

Pesticide mixtures are common in streams with agricultural or urban influence in the watershed. The Pesticide Toxicity Index (PTI) is a screening tool to assess potential aquatic toxicity of complex pesticide mixtures by combining measures of pesticide exposure and acute toxicity in an additive toxic-unit model. The PTI is determined separately for fish, cladocerans, and benthic invertebrates. This study expands the number of pesticides and degradates included in previous editions of the PTI from 124 to 492 pesticides and degradates, and includes two types of PTI for use in different applications, depending on study objectives. The Median-PTI was calculated from median toxicity values for individual pesticides, so is robust to outliers and is appropriate for comparing relative potential toxicity among samples, sites, or pesticides. The Sensitive-PTI uses the 5th percentile of available toxicity values, so is a more sensitive screening-level indicator of potential toxicity. PTI predictions of toxicity in environmental samples were tested using data aggregated from published field studies that measured pesticide concentrations and toxicity to Ceriodaphnia dubia in ambient stream water. C. dubia survival was reduced to ≤ 50% of controls in 44% of samples with Median-PTI values of 0.1–1, and to 0% in 96% of samples with Median-PTI values > 1. The PTI is a relative, but quantitative, indicator of potential toxicity that can be used to evaluate relationships between pesticide exposure and biological condition.

EVALUATING THE EFFECTS OF FLY ASH EXPOSURE ON FISH EARLY LIFE STAGES: FATHEAD MINNOW EMBRYO-LARVAL TESTS

Energy Technology Data Exchange (ETDEWEB)

Greeley Jr, Mark Stephen [ORNL; Elmore, Logan R [ORNL; McCracken, Kitty [ORNL

2012-05-01

On December 22, 2008, a dike containing fly ash and bottom ash in an 84-acre complex of the Tennessee Valley Authority's (TVA) Kingston Steam Plant in East Tennessee failed and released a large quantity of ash into the adjacent Emory River. Ash deposits extended as far as 4 miles upstream (Emory River mile 6) of the Plant, and some ash was carried as far downstream as Tennessee River mile 564 ({approx}4 miles downstream of the Tennessee River confluence with the Clinch River). A byproduct of coal burning power plants, fly ash contains a variety of metals and other elements which, at sufficient concentrations and in specific forms, can be toxic to biological systems. The effects of fly ash contamination on exposed fish populations depend on the magnitude and duration of exposure, with the most significant risk considered to be the effects of specific ash constituents, especially selenium, on fish early life stages. Uptake by adult female fish of fly ash constituents through the food chain and subsequent maternal transfer of contaminants to the developing eggs is thought to be the primary route of selenium exposure to larval fish (Woock and others 1987, Coyle and others 1993, Lemly 1999, Moscatello and others 2006), but direct contact of the fertilized eggs and developing embryos to ash constituents in river water and sediments is also a potential risk factor (Woock and others 1987, Coyle and others 1993, Jezierska and others 2009). To address the risk of fly ash from the Kingston spill to the reproductive health of downstream fish populations, ORNL has undertaken a series of studies in collaboration with TVA including: (1) a field study of the bioaccumulation of fly ash constituents in fish ovaries and the reproductive condition of sentinel fish species in reaches of the Emory and Clinch Rivers affected by the fly ash spill; (2) laboratory tests of the potential toxicity of fly ash from the spill area on fish embryonic and larval development (reported in the

2014 status of the Lake Ontario lower trophic levels

Science.gov (United States)

Holeck, Kristen T.; Rudstam, Lars G.; Hotaling, Christopher; McCullough, Russ D.; Lemon, Dave; Pearsall, Web; Lantry, Jana; Connerton, Michael J.; LaPan, Steve; Biesinger, Zy; Lantry, Brian F.; Walsh, Maureen; Weidel, Brian C.

2015-01-01

Soluble reactive phosphorus (SRP) concentrations have been stable in nearshore and offshore habitats since 1998 (0.4 – 3.3 μg/L). SRP concentrations were low in 2014; Apr/May – Oct mean values were Water Quality Agreement of 1978 for offshore waters of Lake Ontario. TP concentrations were low at both nearshore and offshore locations; Apr/May – Oct mean values from individual sites ranged from 4.6 – 9.1 μg/L. Spring TP has declined significantly in the longer data series (since 1981), but not since 1995 indicating stable nutrient loading into Lake Ontario for nearly two decades. It averaged 7.8 μg/L in the nearshore and 5.6 μg/L in the offshore in 2014.Chlorophyll-a and secchi depth values are indicative of oligotrophic conditions in nearshore and offshore habitats. Offshore summer chlorophyll-a declined significantly in both the short- (2000-2014) and long-term (1981-2014) time series at a rate of 4-6% per year. Nearshore chlorophyll-a increased after 2003 but then declined again after 2009. Epilimnetic chlorophyll-a averaged between 0.6 and 1.6 μg/L across sites with no difference between nearshore and offshore habitats. Apr/May – Oct Secchi depth ranged from 4.0 m to 10.8 m at individual sites and was higher in the offshore (average 9.1 m) than nearshore (5.9 m).In 2014, Apr/May – Oct epilimnetic zooplankton density, size, and biomass were not different between the offshore and the nearshore, and there were no differences in epilimnetic biomass between offshore and nearshore areas for any of the zooplankton groups.Zooplankton density and biomass peaked in September, an atypical pattern. This coincided with peaks in calanoid copepod, daphnid, and Holopedium biomass. Holopedium biomass in the nearshore increased significantly since 1995.The predatory cladoceran Cercopagis continued to be abundant in the summer, peaking at ~10 mg/m3in the offshore. Bythotrephes biomass was at its lowest level since 2005 in both offshore and nearshore habitats

New floristic records in the Balkans: 1

DEFF Research Database (Denmark)

2006-01-01

for countries are: Albania - Eryngium serbicum (80), Bulgaria - Parthenocissus quinquefolia (75), Greece - Gonocytisus dirmilensis (69), Littorella uniflora (9) and Verbena aristigera (75), Greece - (69), (9) and (12); Serbia & Montenegro - Campanula moravica (76), Daphne malyana (82), Lindernia dubia (77......) and Poa timoleontis (79). Gonocytisus dirmilensis and Verbena aristigera are new for Europe. The publication includes contributions by B. Biel & Kit Tan (1-13), N. Böhling (14), D. Dimitrov & V. Vutov (15-28), R. Dimova & V. Vladimirov (29-30), Kit Tan & G. Vold (31-46), Kit Tan, G. Vold, G. Iatrou & G...

Environmental impact of industrial sludge stabilization/solidification products: chemical or ecotoxicological hazard evaluation?

Science.gov (United States)

Silva, Marcos A R; Testolin, Renan C; Godinho-Castro, Alcione P; Corrêa, Albertina X R; Radetski, Claudemir M

2011-09-15

Nowadays, the classification of industrial solid wastes is not based on risk analysis, thus the aim of this study was to compare the toxicity classifications based on the chemical and ecotoxicological characterization of four industrial sludges submitted to a two-step stabilization/solidification (S/S) processes. To classify S/S products as hazardous or non-hazardous, values cited in Brazilian chemical waste regulations were adopted and compared to the results obtained with a battery of biotests (bacteria, alga and daphnids) which were carried out with soluble and leaching fractions. In some cases the hazardous potential of industrial sludge was underestimated, since the S/S products obtained from the metal-mechanics and automotive sludges were chemically classified as non-hazardous (but non-inert) when the ecotoxicity tests showed toxicity values for leaching and soluble fractions. In other cases, the environmental impact was overestimated, since the S/S products of the textile sludges were chemically classified as non-inert (but non-hazardous) while ecotoxicity tests did not reveal any effects on bacteria, daphnids and algae. From the results of the chemical and ecotoxicological analyses we concluded that: (i) current regulations related to solid waste classification based on leachability and solubility tests do not ensure reliable results with respect to environmental protection; (ii) the two-step process was very effective in terms of metal immobilization, even at higher metal-concentrations. Considering that S/S products will be subject to environmental conditions, it is of great interest to test the ecotoxicity potential of the contaminants release from these products with a view to avoiding environmental impact given the unreliability of ecotoxicological estimations originating from chemical analysis. Copyright © 2011 Elsevier B.V. All rights reserved.

Methyl farnesoate synthesis is necessary for the environmental sex determination in the water flea Daphnia pulex.

Science.gov (United States)

Toyota, Kenji; Miyakawa, Hitoshi; Hiruta, Chizue; Furuta, Kenjiro; Ogino, Yukiko; Shinoda, Tetsuro; Tatarazako, Norihisa; Miyagawa, Shinichi; Shaw, Joseph R; Iguchi, Taisen

2015-09-01

Sex-determination systems can be divided into two groups: genotypic sex determination (GSD) and environmental sex determination (ESD). ESD is an adaptive life-history strategy that allows control of sex in response to environmental cues in order to optimize fitness. However, the molecular basis of ESD remains largely unknown. The micro crustacean Daphnia pulex exhibits ESD in response to various external stimuli. Although methyl farnesoate (MF: putative juvenile hormone, JH, in daphnids) has been reported to induce male production in daphnids, the role of MF as a sex-determining factor remains elusive due to the lack of a suitable model system for its study. Here, we establish such a system for ESD studies in D. pulex. The WTN6 strain switches from producing females to producing males in response to the shortened day condition, while the MFP strain only produces females, irrespective of day-length. To clarify whether MF has a novel physiological role as a sex-determining factor in D. pulex, we demonstrate that a MF/JH biosynthesis inhibitor suppressed male production in WTN6 strain reared under the male-inducible condition, shortened day-length. Moreover, we show that juvenile hormone acid O-methyltransferase (JHAMT), a critical enzyme of MF/JH biosynthesis, displays MF-generating activity by catalyzing farnesoic acid. Expression of the JHAMT gene increased significantly just before the MF-sensitive period for male production in the WTN6 strain, but not in the MFP strain, when maintained under male-inducible conditions. These results suggest that MF synthesis regulated by JHAMT is necessary for male offspring production in D. pulex. Our findings provide novel insights into the genetic underpinnings of ESD and they begin to shed light on the physiological function of MF as a male-fate determiner in D. pulex. Copyright © 2015 Elsevier Ltd. All rights reserved.

Effects of charge and surface ligand properties of nanoparticles on oxidative stress and gene expression within the gut of Daphnia magna

Energy Technology Data Exchange (ETDEWEB)

Dominguez, Gustavo A.; Lohse, Samuel E.; Torelli, Marco; Murphy, Catherine; Hamers, Robert J.; Orr, Galya; Klaper, Rebecca D.

2015-05-01

Concern has been raised regarding the current and future release of engineered nanomaterials into aquatic environments from industry and other sources. However, not all nanomaterials may cause an environ-mental impact and identifying which nanomaterials may be of greatest concern has been difficult. It is thought that the surface groups of a functionalized nanoparticles (NPs) may play a significant role in determining their interactions with aquatic organisms, but the way in which surface properties of NPs impact their toxicity in whole organisms has been minimally explored. A major point of interaction of NPs with aquatic organisms is in the gastrointestinal tract as they ingest particulates from the water column or from the sediment. The main goal of this study was to use model gold NP (AuNPs) to evaluate the potential effects of the different surfaces groups on NPs on the gut of an aquatic model organism, Daphnia magna. In this study, we exposed daphnids to a range of AuNPs concentrations and assessed the impact of AuNP exposure in the daphnid gut by measuring reactive oxygen species (ROS) production and expression of genes associated with oxidative stress and general cellular stress: glutathione S-transferase(gst), catalase (cat), heat shock protein 70 (hsp70), and metallothionein1 (mt1). We found ROS formation and gene expression were impacted by both charge and the specific surface ligand used. We detected some degree of ROS production in all NP exposures, but positively charged AuNPs induced a greater ROS response. Similarly, we observed that, compared to controls, both positively charged AuNPs and only one negatively AuNP impacted expression of genes associated with cellular stress. Finally, ligand-AuNP exposures showed a different toxicity and gene expression profile than the ligand alone, indicating a NP specific effect.

Clinch River breeder reactor sodium fire protection system design and development

International Nuclear Information System (INIS)

Foster, K.W.; Boasso, C.J.; Kaushal, N.N.

1984-01-01

To assure the protection of the public and plant equipment, improbable accidents were hypothesized to form the basis for the design of safety systems. One such accident is the postulated failure of the Intermediate Heat Transfer System (IHTS) piping within the Steam Generator Building (SGB), resulting in a large-scale sodium fire. This paper discusses the design and development of plant features to reduce the consequences of the accident to acceptable levels. Additional design solutions were made to mitigate the sodium spray contribution to the accident scenario. Sodium spill tests demonstrated that large sodium leaks can be safely controlled in a sodium-cooled nuclear power plant

U.S. – Russia Relations And Arms Control: Breating The Clinch

Directory of Open Access Journals (Sweden)

N. I. Bubnova

2017-01-01

Full Text Available The article examines the new U.S. administration’s outlook on arms control, analyses the existing problems in this field and possible ways for their resolution, compares Donald Trump’s statements on military and political issues with the president’s first steps in this area, and offers forecasts and recommendations on the prospects for U.S.-Russia cooperation in arms limitations and reductions. The author proceeds from the assumption that arms control is a key component in measures to ensure international security. Even if the current tense U.S.- Russian relations provide little room for maneuver and the internal political struggle in DC sets limits on the possible bilateral measures, Moscow should nevertheless take initiative in putting forward a set of proposals to reduce weapons, especially in the very sensitive and risk-prone nuclear field. This will allow in future negotiations to proceed from options that will take into account Russian interests and the specifics of Russia’s weapons systems, while at the same time showing the resolve of the nuclear superpowers to reduce existing threats through negotiations and leading the way for other countries to join. Taking into account the importance of arms control, all efforts should be made to enforce the existing treaties, consolidate the current international treaty-based legal system, and work further on new arms control and arms reduction agreements.

Primary pipe rupture accident analysis for the Clinch River Breeder Reactor

Energy Technology Data Exchange (ETDEWEB)

Albright, D.C.; Bari, R.A.

1976-04-01

In this report, the thermal transient response of the CRBR to a severe primary coolant flow perturbation, initiated by a rupture of the primary heat transport system piping, is analyzed. This hypothetical accident is studied under the further assumption that the plant protection system does function according to current design descriptions for the CRBR. Although a brief discussion of an unprotected (no scram) pipe rupture accident is presented, the major emphasis of the present report is on the protected accident.

Clinch River Breeder Reactor Plant. License application, statement of general information

International Nuclear Information System (INIS)

1975-01-01

Application is made for a reactor facility consisting of a liquid metal cooled reactor and steam generator system, a steam turbine driven electric generating system, electrical switchyard, and related auxiliaries and supporting structures. The primary system is located in an inert atmosphere in shielded vaults within a containment structure. Sodium coolant is used to remove heat from the core and radial blanket. Heat from the primary sodium is transferred in heat exchangers to non radioactive sodium which is used to convert feed-water into steam which is superheated to drive a tandem-compound generator. A single shaft multi-stage turbine generator produces 380 MW(e) with steam conditions of 1450 psig at 900 0 F. Fuel is sintered ceramic pellets of mixed uranium-plutonium oxides encapsulated in stainless steel. There are 198 fuel assemblies with each assembly consisting of 217 fuel rods placed in a hexagonal channel. Plutonium enrichment ranges from 1817 to 32.0 percent by weight. Axial blanket sections contain depleted UO 2 with 99.8 percent 238 U and 0.2 percent 235 U by weight. The proposed location of the plant is within the corporate limits of the city of Oak Ridge in Roane County, Tennessee. (U.S.)

Primary pipe rupture accident analysis for the Clinch River Breeder Reactor

International Nuclear Information System (INIS)

Albright, D.C.; Bari, R.A.

1976-04-01

In this report, the thermal transient response of the CRBR to a severe primary coolant flow perturbation, initiated by a rupture of the primary heat transport system piping, is analyzed. This hypothetical accident is studied under the further assumption that the plant protection system does function according to current design descriptions for the CRBR. Although a brief discussion of an unprotected (no scram) pipe rupture accident is presented, the major emphasis of the present report is on the protected accident

Field sampling and analysis plan for the remedial investigation of Waste Area Grouping 2 at Oak Ridge National Laboratory, Oak Ridge, Tennessee. Environmental Restoration Program

Energy Technology Data Exchange (ETDEWEB)

Boston, H.L.; Ashwood, T.L.; Borders, D.M.; Chidambariah, V.; Downing, D.J.; Fontaine, T.A.; Ketelle, R.H.; Lee, S.Y.; Miller, D.E.; Moore, G.K.; Suter, G.W.; Tardiff, M.F.; Watts, J.A.; Wickliff, D.S.

1992-02-01

This field sampling and analysis (S & A) plan has been developed as part of the Department of Energy`s (DOE`s) remedial investigation (RI) of Waste Area Grouping (WAG) 2 at Oak Ridge National Laboratory (ORNL) located in Oak Ridge, Tennessee. The S & A plan has been written in support of the remedial investigation (RI) plan for WAG 2 (ORNL 1990). WAG 2 consists of White Oak Creek (WOC) and its tributaries downstream of the ORNL main plant area, White Oak Lake (WOL), White Oak Creek embayment (WOCE) on the Clinch River, and the associated floodplain and subsurface environment (Fig. 1.1). The WOC system is the surface drainage for the major ORNL WAGs and has been exposed to a diversity of contaminants from operations and waste disposal activities in the WOC watershed. WAG 2 acts as a conduit through which hydrologic fluxes carry contaminants from upgradient areas to the Clinch River. Water, sediment, soil, and biota in WAG 2 are contaminated and continue to receive contaminants from upgradient WAGs. This document describes the following: an overview of the RI plan, background information for the WAG 2 system, and objectives of the S & A plan; the scope and implementation of the first 2 years of effort of the S & A plan and includes recent information about contaminants of concern, organization of S & A activities, interactions with other programs, and quality assurance specific to the S & A activities; provides details of the field sampling plans for sediment, surface water, groundwater, and biota, respectively; and describes the sample tracking and records management plan.

Uptake of cadmium from a dietary and soluble source by the crustacean Daphnia magna

International Nuclear Information System (INIS)

Carney, G.C.; Shore, P.; Chandra, H.

1986-01-01

Daphnia were exposed to radioactively labeled cadmium in solution and in the presence of Chlorella which had been preloaded with the metal to varying extents. Illuminated algal cells retained the cadmium and greatly reduced its availability to the daphnids. Autoradiographic evidence was obtained which implicated the exoskeleton as a major sink for the cadmium taken up from solution. Cadmium in solution at a concentration close to the 48 hr LC 50 level did not affect respiration during the first 6 hr of exposure. Retention patterns were similar, regardless of the source of cadmium, but ecdysis resulted in a considerable loss of body burden provided that this had been acquired via a predominantly soluble route

Design data and safety features of commerical nuclear power plant

International Nuclear Information System (INIS)

Heddleson, F.A.

1976-06-01

Design data, safety features, and site characteristics are summarized for 34 nuclear power units in 17 power stations in the United States. Six pages of data are presented for each plant, consisting of thermal-hydraulic and nuclear factors, containment features, emergency-core-cooling systems, site features, circulating water system data, and miscellaneous factors. An aerial perspective is also presented for each plant. This volume covers Light Water Reactors (LWRs) with dockets 50-508 through 50-549, four HTGRs--50-171, 50-267, 50-450/451, 50-463/464, the Atlantic Floating Station 50-477/478, and the Clinch River Breeder 50-537

Automated system for loading nuclear fuel pins

International Nuclear Information System (INIS)

Marshall, J.L.

1983-10-01

A completely automatic and remotely controlled fuel pin fabrication system is being designed by the Westinghouse Hanford Company. The Pin Operations System will produce fuel pins for the Fast Flux Test Facility (FFTF) and the Clinch River Breeder Reactor Plant (CRBRP). The system will assemble fuel pin components into cladding tubes in a controlled environment. After fuel loading, the pins are filled with helium, the tag gas capsules are inserted, and the top end cap welded. Following welding, the pins are surveyed to assure they are free of contamination and then the pins are helium leak tested

Sodium-water wastage and reactions program performed by general electric in support of the US. AEC LMFBR steam generator development

International Nuclear Information System (INIS)

Greene, D.A.

1975-01-01

This paper constitutes an interim report on the sodium-water reaction programs performed, using the GE-SOWAT, GE-SMALL LEAK BEHAVIOR RIG, and GE-PTTR facilities in support of LMFBR steam generator development and its application to the Clinch River Breeder Reactor Plant. Test data from these rigs are presented, including wastage data as a function of water injection rate, sodium temperature, and orifice geometry. Initial results for self-wastage of defects under prototypical conditions, and from proof-of-principle tests of a protected heat transfer tube concept are also presented. An analytical basis for wastage phenomena is suggested. (author)

Carter's breeder policy has failed, claims Westinghouse manager

International Nuclear Information System (INIS)

Anon.

1979-01-01

Nuclear nations developing liquid metal fast breeder reactor (LMFBR) technology have not been dissuaded by President Carter's efforts to stop the breeder program as a way to control the proliferation of nuclear weapons. There is no evidence that Carter's policy of moral persuasion has had any impact on their efforts. A review of the eight leading countries cites their extensive progress in the areas of breeder technology and fuel reprocessing, while the US has made only slight gains. The Fast Flux Test Facility at Hanford is near completion, but the Clinch River project has been slowed to a minimum

Flood analyses for Department of Energy Y-12, ORNL and K-25 Plants. Flood analyses in support of flood emergency planning

International Nuclear Information System (INIS)

1995-05-01

The study involved defining the flood potential and local rainfall depth and duration data for the Department of Energy's (DOE) Y-12, Oak Ridge National Laboratory (ORNL), and K-25 plants. All three plants are subject to flooding from the Clinch River. In addition, the Y-12 plant is subject to flooding from East Fork Poplar and Bear Creeks, the ORNL plant from Whiteoak Creek and Melton Branch, and the K-25 plant from Poplar Creek. Determination of flood levels included consideration of both rainfall events and postulated failures of Norris and Melton Hill Dams in seismic events

Radiation protection/shield design: a need for a systems approach

International Nuclear Information System (INIS)

Disney, R.K.

1977-01-01

Radiation protection/shielding design of a nuclear facility requires a coordinated effort of many engineering disciplines to meet the requirements imposed by regulations. The system approach to Clinch River Breeder Reactor Plant (CRBRP) radiation protection is described, and the program developed to implement this approach is defined. In addition, the principal shielding design problems for LMFBR nuclear reactor systems are discussed in relation to LWR nuclear reactor system shielding designs. The methodology used to analyze these problems in the U.S. LMFBR program, the resultant design solutions, and the experimental verification of these designs and/or methods are discussed

Prism reactor system design and analysis of postulated unscrammed events

International Nuclear Information System (INIS)

Van Tuyle, G.J.; Slovik, G.C.

1991-08-01

Key safety characteristics of the PRISM reactor system include the passive reactor shutdown characteristic and the passive shutdown heat removal system, RVACS. While these characteristics are simple in principle, the physical processes are fairly complex, particularly for the passive reactor shutdown. It has been possible to adapt independent safety analysis codes originally developed for the Clinch River Breeder Reactor review, although some limitations remain. In this paper, the analyses of postulated unscrammed events are discussed, along with limitations in the predictive capabilities and plans to correct the limitations in the near future. 6 refs., 4 figs

PRISM reactor system design and analysis of postulated unscrammed events

International Nuclear Information System (INIS)

Van Tuyle, G.J.; Slovik, G.C.; Rosztoczy, Z.; Lane, J.

1991-01-01

Key safety characteristics of the PRISM reactor system include the passive reactor shutdown characteristics and the passive shutdown heat removal system, RVACS. While these characteristics are simple in principle, the physical processes are fairly complex, particularly for the passive reactor shutdown. It has been possible to adapt independent safety analysis codes originally developed for the Clinch River Breeder Reactor review, although some limitations remain. In this paper, the analyses of postulated unscrammed events are discussed, along with limitations in the predictive capabilities and plans to correct the limitations in the near future. 6 refs., 4 figs

Sodium-water wastage and reactions program performed by general electric in support of the US. AEC LMFBR steam generator development

Energy Technology Data Exchange (ETDEWEB)

Greene, D A

1975-07-01

This paper constitutes an interim report on the sodium-water reaction programs performed, using the GE-SOWAT, GE-SMALL LEAK BEHAVIOR RIG, and GE-PTTR facilities in support of LMFBR steam generator development and its application to the Clinch River Breeder Reactor Plant. Test data from these rigs are presented, including wastage data as a function of water injection rate, sodium temperature, and orifice geometry. Initial results for self-wastage of defects under prototypical conditions, and from proof-of-principle tests of a protected heat transfer tube concept are also presented. An analytical basis for wastage phenomena is suggested. (author)

Technology which led to the westinghouse inherently safe liquid metal reactor

International Nuclear Information System (INIS)

Schmidt, J.E.; Coffield, R.D.; Doncals, R.A.; Kalinowski, J.E.; Markley, R.A.

1985-01-01

The Fast Flux Test Facility (FFTF) and the Clinch River Breeder Reactor programs resulted in an understanding of liquid metal reactor behavior that is being used to design inherent safety capability into liquid metal reactors. Technological advances give the same beneficial operating characteristics of conventional liquid metal reactors, however, the addition of inherently safe design features precludes the initiation of hypothetical core disruptive accidents. These innovative features permit inherent safety capability to be demonstrated with more than adequate margins. Also, the variety of inherent safety features provides the designers with options in selecting inherent design features for a specific reactor application

LMFBR intermediate-heat-exchanger experience

International Nuclear Information System (INIS)

Cho, S.M.; Beaver, T.R.

1983-01-01

This paper presents developmental and operating experience of large Intermediate Heat Exchangers (IHX's) in US from the Fast Flux Test Facility (FFTF) to the Clinch River Breeder Reactor Plant (CRBRP) to the Large Development Plant (LDP). Design commonalities and deviations among these IHX's are synopsized. Various developmental tests that were conducted in the areas of hydraulic, structural and mechanical design are also presented. The FFTF is currently operating. Performance data of the FFTF IHXs are reviewed, and comparisons between actual and predicted performances are made. The results are used to assess the adequacy of IHX designs

Earth sciences

International Nuclear Information System (INIS)

Tamura, T.

1978-01-01

The following waste management studies were conducted: assessment of ORNL radioactive solid waste disposal practices and facilities; assessment of stream monitoring network in White Oak Creek watershed; discharge of 90 Sr from burial ground 4; evaluation of burial ground corrective measures; halocarbons as ground water tracers; 60 Co transport mechanisms; 60 Co adsorption kinetics; and soil chromatograph K/sub d/ values. Other studies were conducted on cycling and transport of fusion-activation products in the terrestrial environment; Clinch River inventory; biological denitrification; leachates from stored fossil-fuel solids; coal storage piles; and disposal of solid wastes

Cover gas seals: FFTF-LMFBR seal test program

International Nuclear Information System (INIS)

Kurzeka, W.; Oliva, R.; Welch, T.S.; Shimazaki, T.

1974-01-01

The objectives of this program are to: (1) conduct static and dynamic tests to demonstrate or determine the mechanical performance of full-size (cross section) FFTF fuel transfer machine and reactor vessel head seals intended for use in a sodium vapor-inert gas environment, (2) demonstrate that these FFTF seals or new seal configurations provide acceptable fission product and cover gas retention capabilities at Clinch River Breeder Reactor Plant (CRBRP) operating environmental conditions other than radiation, and (3) develop improved seals and seal technology for the CRBRP to support the national objective to reduce all atmospheric contaminations to low levels

A Novel Double Heterozygous Hb D-Punjab/Hb J-Meerut Hemoglobinopathy.

Science.gov (United States)

Chandra, Dinesh; Tyagi, Seema; Deka, Roopam; Chauhan, Richa; Seth, Tulika; Saxena, Renu; Pati, H P

2017-12-01

A comprehensive laboratory diagnosis of hemoglobinopathies forms an integral part in workup of disorders of globin chain synthesis. Clinical findings, complete blood counts, peripheral smear examination along with hemoglobin (Hb) electrophoresis and/or cation exchange high performance liquid chromatography findings and parental study helps to clinch a final diagnosis. Compound heterozygous hemoglobinopathy presents with variable clinical findings and some of them are picked up on screening tests done as part of routine antenatal workup. Here we report a rare double heterozygous hemoglobinopathy of Hb D-Punjab and Hb J-Meerut in a 35 year antenatal female.

Experimental studies of 350 Mw(e) heterogeneous LMFBR cores at ZPPR

International Nuclear Information System (INIS)

Collins, P.J.; McFarlane, H.F.; Beck, C.L.; Lineberry, M.J.; Carpenter, S.G.; Ducat, G.A.; Gasidlo, J.M.; Goin, R.W.

1979-01-01

The annular heterogeneous concept has been adopted for the Clinch River Breeder Reactor. To provide verification of design and safety analysis, fourteen core configurations were studied at ZPPR. The results and analysis of the early assemblies, ZPPR-7A, 7B, and 7C, have been previously reported and only the more significant conclusions are summarized here. Later assemblies, 7D to 7H, were designed to provide data on variations of control rod and internal blanket arrangements. Assemblies 8A to 8E provided data on the replacement of uranium by thorium in various blanket subassemblies

Ileosigmoid knot: A case report

International Nuclear Information System (INIS)

Baheti, Akshay D; Patel, Darshana; Hira, Priya; Babu, Donald

2011-01-01

The ileosigmoid knot is an uncommon but life-threatening cause of closed loop intestinal obstruction. Its treatment is different from a simple volvulus in that it has to be operated upon immediately. Preoperative CT scan diagnosis and prompt treatment can lead to a good outcome. Findings of simultaneous ileal and sigmoid ischemia with non-ischemic colon interposed in between should, in an appropriate clinical setting, indicate this condition. The presence of the whirl sign, medially deviated distal descending colon and cecum, and mesenteric vascular structures from the superior mesenteric vessels that converge toward the sigmoid colon on CT scan help clinch the diagnosis

Ecotoxicological assessment of the pharmaceutical compound Triclosan to freshwater invertebrates with emphasis to spiked sediment tests; Avaliacao ecotoxicologica do farmaco Triclosan para invertebrados de agua doce com enfase em ensaios com sedimento marcado ('spiked sediment')

Energy Technology Data Exchange (ETDEWEB)

Pusceddu, Fabio Hermes

2009-07-01

The increasing of Pharmaceutical and Personal Care Products (PPCPs) occurrence in the aquatic environment cause adverse effects on the human health and aquatic communities. The environmental risk of the PPCPs associated with the possibility of synergic effects between PCPPs and the increase of the use of synthetic organic compounds, unchained a great concern on the toxic potential to biota aquatic. Triclosan (5-chloro-2-(2,4-dichlorophenoxy)-phenol) is a pharmaceutical compound widely used due your antibacterial mechanism effect, found in at least 932 products such as shampoos, toilet soaps, deodorants, lotions, toothpaste, detergents, socks and underwear, among others. Currently, studies about the Triclosan toxicity in the water and, mainly in the sediment, are poorly. We have the knowledge that the photodegradation of this product results into dichlorodibenzo-p-dioxin, and now it has great discussion on environmental agencies, like EPA, about the release or restriction of this product. The aim of this work is to assess the effects of Triclosan on mortality of insect larvae Chironomus xanthus and mortality and reproduction inhibition of microcrustacea Ceriodaphnia dubia exposed to Triclosan spiked sediments based on standard methods EPA and OECD. The EC50;96H obtained on acute toxicity tests with C. xanthus was 45,26 mg.Kg{sup -1}. The chronic toxicity tests with C. dubia using spiked sediments were performed following the procedure in Burton and MacPherson (1995). A no-observed-effect concentrations and lowest-observed-effect concentration were 5,78 e 6,94 mg.Kg{sup -1}, respectively. (author)

Assessment of sediments from Tiete River - toxicity and trace elements - from Salesopolis to Suzano counties, Sao Paulo, Brazil

International Nuclear Information System (INIS)

Alegre, Gabriel F.; Borrely, Sueli; Nascimento, Thuany M.; Favaro, Deborah I.T.

2009-01-01

In the present study, sediment samples from the Tiete River were evaluated for toxicity and trace metals (5 sampling sites). The studied region includes Salesopolis to Suzano and surroundings, a highly industrialized area. The study involved toxicity evaluation (sediment, elutriate and pore-water) and the distribution of some major, trace and rare earth elements on sediments. Multielemental analysis was carried out by instrumental neutron activation analysis (INAA) and total mercury by cold vapor atomic absorption technique (CV AAS). The concentration values obtained for the metals As, Cr, Hg and Zn in the sediment samples were compared to the Canadian Council of Minister of the Environment (CCME) oriented values (TEL and PEL). Regarding toxicity, whole sediments and elutriate fractions were evaluated using chronic assays for Ceriodaphnia dubia, while the pore water was carried out for Vibrio fischeri toxicity assays. These assays followed Brazilian Standardized Methods (ABNT). Whole sediments and elutriate evidenced negative biological effects, even at Salesopolis county, the control site (less impacted area). The worst effects were obtained at Mogi das Cruzes and Suzano counties (sampling stations 3 and 4). The elutriate fractions collected at the same stations showed acute toxicity in two of three samples (C. dubia). When pore water was evaluated, a toxicity gradient which increased as the river flowed through Mogi das Cruzes county was obtained. Regarding toxic metal contents in the sediment samples points 3 and 4 exceeded the TEL oriented values for As, Cr, Hg and Zn and point 4 also exceeded the PEL values for all these elements. (author)

The aquatic toxicity and chemical forms of coke plant effluent cyanide -- Implications for discharge limits

International Nuclear Information System (INIS)

Garibay, R.; Rupnow, M.; Godwin-Saad, E.; Hall, S.

1995-01-01

Cyanide is present in treated cokemaking process waters at concentrations as high as 8.0 mg/L. In assessing options for managing the discharge of a treated effluent, the development and implementation of discharge limits for cyanide became a critical issue. A study was initiated to evaluate possible alternatives to cyanide permit limits at the US Steel Gary Works Facility. The objectives of the study were to: (1) evaluation the forms of cyanide present in coke plant effluent; (2) determine whether these forms of cyanide are toxic to selected aquatic organisms; (3) compare the aquatic toxicity of various chemical forms of cyanide; (4) identify if the receiving water modifies cyanide bioavailability; and (5) confirm, with respect to water quality-based effluent limits, an appropriate analytical method for monitoring cyanide in a coke plant effluent. The results of aquatic toxicity tests and corresponding analytical data are presented. Toxicity tests were conducted with various pure chemical forms of cyanide as well as whole coke plant effluent (generated from a pilot-scale treatment system). Test species included the fathead minnow (Pimephales promelas), rainbow trout (Oncorhynchus mykiss), Ceriodaphnia dubia (C. dubia) and Daphnia magna (D. magna). Analytical measurements for cyanide included total, weak acid dissociable, diffusible cyanide and selected metal species of cyanide. The findings presented by the paper are relevant with respect to the application of cyanide water quality criteria for a coke plant effluent discharge, the translation of these water quality-based effluent limits to permit limits, and methods for compliance monitoring for cyanide

Ecotoxicological assessment of the pharmaceutical compound Triclosan to freshwater invertebrates with emphasis to spiked sediment tests; Avaliacao ecotoxicologica do farmaco Triclosan para invertebrados de agua doce com enfase em ensaios com sedimento marcado ('spiked sediment')

Energy Technology Data Exchange (ETDEWEB)

Pusceddu, Fabio Hermes

2009-07-01

The increasing of Pharmaceutical and Personal Care Products (PPCPs) occurrence in the aquatic environment cause adverse effects on the human health and aquatic communities. The environmental risk of the PPCPs associated with the possibility of synergic effects between PCPPs and the increase of the use of synthetic organic compounds, unchained a great concern on the toxic potential to biota aquatic. Triclosan (5-chloro-2-(2,4-dichlorophenoxy)-phenol) is a pharmaceutical compound widely used due your antibacterial mechanism effect, found in at least 932 products such as shampoos, toilet soaps, deodorants, lotions, toothpaste, detergents, socks and underwear, among others. Currently, studies about the Triclosan toxicity in the water and, mainly in the sediment, are poorly. We have the knowledge that the photodegradation of this product results into dichlorodibenzo-p-dioxin, and now it has great discussion on environmental agencies, like EPA, about the release or restriction of this product. The aim of this work is to assess the effects of Triclosan on mortality of insect larvae Chironomus xanthus and mortality and reproduction inhibition of microcrustacea Ceriodaphnia dubia exposed to Triclosan spiked sediments based on standard methods EPA and OECD. The EC50;96H obtained on acute toxicity tests with C. xanthus was 45,26 mg.Kg{sup -1}. The chronic toxicity tests with C. dubia using spiked sediments were performed following the procedure in Burton and MacPherson (1995). A no-observed-effect concentrations and lowest-observed-effect concentration were 5,78 e 6,94 mg.Kg{sup -1}, respectively. (author)

Cuticle Fatty Acid Composition and Differential Susceptibility of Three Species of Cockroaches to the Entomopathogenic Fungi Metarhizium anisopliae (Ascomycota, Hypocreales).

Science.gov (United States)

Gutierrez, Alejandra C; Gołębiowski, Marek; Pennisi, Mariana; Peterson, Graciela; García, Juan J; Manfrino, Romina G; López Lastra, Claudia C

2015-04-01

Differences in free fatty acids (FFAs) chemical composition of insects may be responsible for susceptibility or resistance to fungal infection. Determination of FFAs found in cuticular lipids can effectively contribute to the knowledge concerning insect defense mechanisms. In this study, we have evaluated the susceptibility of three species of cockroaches to the entomopathogenic fungi Metarhizium anisopliae (Metschnikoff) Sorokin by topical application. Mortality due to M. anisopliae was highly significant on adults and nymphs of Blattella germanica L. (Blattodea: Blattellidae). However, mortality was faster in adults than in nymphs. Adults of Blatta orientalis L. (Blattodea: Blattidae) were not susceptible to the fungus, and nymphs of Blaptica dubia Serville (Blattodea: Blaberidae) were more susceptible to the fungus than adults. The composition of cuticular FFAs in the three species of cockroaches was also studied. The analysis indicated that all of the fatty acids were mostly straight-chain, long-chain, saturated or unsaturated. Cuticular lipids of three species of cockroaches contained 19 FFAs, ranging from C14:0 to C24:0. The predominant fatty acids found in the three studied species of cockroaches were oleic, linoleic, palmitic, and stearic acid. Only in adults of Bl. orientalis, myristoleic acid, γ-linolenic acid, arachidic acid, dihomolinoleic acid, and behenic acid were identified. Lignoceric acid was detected only in nymphs of Bl. orientalis. Heneicosylic acid and docosahexaenoic acid were identified in adults of Ba. dubia. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Ecotoxicological assessment of the pharmaceutical compound Triclosan to freshwater invertebrates with emphasis to spiked sediment tests

International Nuclear Information System (INIS)

Pusceddu, Fabio Hermes

2009-01-01

The increasing of Pharmaceutical and Personal Care Products (PPCPs) occurrence in the aquatic environment cause adverse effects on the human health and aquatic communities. The environmental risk of the PPCPs associated with the possibility of synergic effects between PCPPs and the increase of the use of synthetic organic compounds, unchained a great concern on the toxic potential to biota aquatic. Triclosan (5-chloro-2-(2,4-dichlorophenoxy)-phenol) is a pharmaceutical compound widely used due your antibacterial mechanism effect, found in at least 932 products such as shampoos, toilet soaps, deodorants, lotions, toothpaste, detergents, socks and underwear, among others. Currently, studies about the Triclosan toxicity in the water and, mainly in the sediment, are poorly. We have the knowledge that the photodegradation of this product results into dichlorodibenzo-p-dioxin, and now it has great discussion on environmental agencies, like EPA, about the release or restriction of this product. The aim of this work is to assess the effects of Triclosan on mortality of insect larvae Chironomus xanthus and mortality and reproduction inhibition of microcrustacea Ceriodaphnia dubia exposed to Triclosan spiked sediments based on standard methods EPA and OECD. The EC50;96H obtained on acute toxicity tests with C. xanthus was 45,26 mg.Kg -1 . The chronic toxicity tests with C. dubia using spiked sediments were performed following the procedure in Burton and MacPherson (1995). A no-observed-effect concentrations and lowest-observed-effect concentration were 5,78 e 6,94 mg.Kg -1 , respectively. (author)

The chronic toxicity of sodium bicarbonate, a major component of coal bed natural gas produced waters

Science.gov (United States)

Farag, Aïda M.; Harper, David D.

2014-01-01

Sodium bicarbonate (NaHCO3) is the principal salt in coal bed natural gas produced water from the Powder River Structural Basin, Wyoming, USA, and concentrations of up to 3000 mg NaHCO3/L have been documented at some locations. No adequate studies have been performed to assess the chronic effects of NaHCO3 exposure. The present study was initiated to investigate the chronic toxicity and define sublethal effects at the individual organism level to explain the mechanisms of NaHCO3 toxicity. Three chronic experiments were completed with fathead minnows (Pimephales promelas), 1 with white suckers (Catostomus commersoni), 1 with Ceriodaphnia dubia, and 1 with a freshwater mussel, (Lampsilis siliquoidea). The data demonstrated that approximately 500 mg NaHCO3/L to 1000 mg NaHCO3/L affected all species of experimental aquatic animals in chronic exposure conditions. Freshwater mussels were the least sensitive to NaHCO3 exposure, with a 10-d inhibition concentration that affects 20% of the sample population (IC20) of 952 mg NaHCO3/L. The IC20 for C. dubia was the smallest, at 359 mg NaHCO3/L. A significant decrease in sodium–potassium adenosine triphosphatase (Na+/K+ ATPase) together with the lack of growth effects suggests that Na+/K+ ATPase activity was shut down before the onset of death. Several histological anomalies, including increased incidence of necrotic cells, suggested that fish were adversely affected as a result of exposure to >450 mg NaHCO3/L.

Study of the microencapsulation of camu-camu (Myrciaria dubia) juice.

Science.gov (United States)

Dib Taxi, C M A; de Menezes, H C; Santos, A B; Grosso, C R F

2003-01-01

The camu-camu, like many other Amazonian fruits, shows an excellent potential for use due to its high vitamin C content, and the use of these natural resources could result in greater development of the Amazonian region. Few studies have been conducted with this fruit, and such studies are necessary in order to develop the required technology to allow for its utilization, thus avoiding or at least decreasing wastage of such a rich raw material. The principle objective of this study was to develop a process for the microencapsulation of camu-camu juice, optimizing the operational conditions. The processing conditions consisted of blanching at a temperature of 95 +/- 2 degrees C for 2 min, followed by cooling in an ice bath and juice extraction using a brush type depulper. The juice was dried with gum arabic or malt dextrin in a mini-spray dryer using an air entry temperature of between 100-160 degrees C and wall material concentration varying between 5-35%, in accordance with a factorial experimental design. Both the air entry temperature and the amount of wall material, plus the interaction between the two, gave significant positive effects at the level of 5% probability on the yield of juice powder. The optimum conditions for juice yield and vitamin C retention were established as 15% wall material and an air entry temperature of 150 degrees C.

Assessing the fate and toxicity of Thallium I and Thallium III to three aquatic organisms.

Science.gov (United States)

Rickwood, C J; King, M; Huntsman-Mapila, P

2015-05-01

Thallium has been shown to significantly increase in both water and aquatic biota after exposure to metal mine effluent, however, there is a lack of knowledge as to its fate and effect in the aquatic environment. The objectives of this project were to assess (1) fate of thallium by conducting speciation analysis and determining the influence of water quality on toxicity and (2) effects of thallium (I) and (III) on three aquatic species; the algae, Pseudokirchneriella subcapitata, the invertebrate Ceriodaphnia dubia and the vertebrate Pimephales promelas. Speciation analysis proved challenging with poor recovery of thallium (I), however analysis with solutions >125μg/L revealed that over a 7-d period, recovery of thallium (III) was less than 15%, suggesting that the majority of thallium (III) was converted to Thallium (I). It was only in fresh solutions where recovery of Thallium (III) was greater than 80%. The lowest IC25s generated during our effects assessment for both Thallium (I) and (III) were more than 10-fold greater than the highest concentration recorded in receiving environments (8μg/L) and more than 100-fold greater than the current guideline (0.8μg/L). To assess the influence of water quality on thallium toxicity, the concentrations of both potassium and calcium were reduced in dilution water. When potassium was reduced for both C. dubia and P. subcapitata tests, the lowest IC25 generated was 5-fold higher than the current guideline, but within the range of concentrations reported in receiving environments for both Thallium (I) and (III). When calcium was reduced in dilution water, toxicity only increased in the Tl (III) tests with C. dubia; the IC25 for Tl(III), similar to the exposures conducted with reduced potassium, was within the range of total thallium concentrations reported in the receiving environment. Without an accurate, repeatable method to assess thallium speciation at low concentrations it is not possible to draw any firm conclusions

Ecotoxicological assessment of sediments from Tiete river between Salesopolis and Suzano, SP (Brazil); Avaliacao ecotoxicologica de sedimentos do rio Tiete, entre os municipios de Salesopolis e Suzano, SP

Energy Technology Data Exchange (ETDEWEB)

Alegre, Gabriel Fonseca

2009-07-01

Once introduced into the aquatic environment, many substances can bind or be adsorbed by organic particles in suspension. Depending on the river morphology and hydrological conditions, these particles in suspension containing the contaminants can be deposited along its course, becoming part of the bottom sediments, making them actual sinks and often a source of contamination for the water column and benthic organisms. In the assessment of water, sediment has been one of the most important indicators of the contamination levels in aquatic ecosystems, representing the deposition of contaminants in the environment that occurred over the years and even decades. The Tiete River cross the Sao Paulo state, however, in the metropolitan region of Sao Paulo, the river shows the most severe degradation. In the region of Salesopolis, the waters of the Tiete River are used for public supply, but across the city of Mogi das Cruzes the water quality decreases significantly. Considering the importance of the Tiete river and the sediment for the aquatic biota, this study aimed to evaluate the toxicity of the sediment at five points along the Tiete river, between the cities of Salesopolis and Suzano, Sao Paulo. Four sampling were carried out: two in the summer (rainy season) and two in winter (dry season). The whole sediment was assessed by acute and chronic toxicity tests with Hyalella azteca and Ceriodaphnia dubia, respectively, the elutriate was assessed by chronic toxicity test using C. dubia, while the porewater was evaluated by acute toxicity test with Vibrio fischeri. Samples of river water were also evaluated for chronic toxicity tests with C. dubia. The quantification of metals and hydrocarbons in sediment samples was also carried out in order to correlate the biological effects with the chemical contamination. The obtained results with the whole sediment test indicate Mogi das Cruzes and Suzano cities as the most toxic sites and also as the sites with the highest

Ecotoxicological assessment of sediments from Tiete river between Salesopolis and Suzano, SP (Brazil)

International Nuclear Information System (INIS)

Alegre, Gabriel Fonseca

2009-01-01

Once introduced into the aquatic environment, many substances can bind or be adsorbed by organic particles in suspension. Depending on the river morphology and hydrological conditions, these particles in suspension containing the contaminants can be deposited along its course, becoming part of the bottom sediments, making them actual sinks and often a source of contamination for the water column and benthic organisms. In the assessment of water, sediment has been one of the most important indicators of the contamination levels in aquatic ecosystems, representing the deposition of contaminants in the environment that occurred over the years and even decades. The Tiete River cross the Sao Paulo state, however, in the metropolitan region of Sao Paulo, the river shows the most severe degradation. In the region of Salesopolis, the waters of the Tiete River are used for public supply, but across the city of Mogi das Cruzes the water quality decreases significantly. Considering the importance of the Tiete river and the sediment for the aquatic biota, this study aimed to evaluate the toxicity of the sediment at five points along the Tiete river, between the cities of Salesopolis and Suzano, Sao Paulo. Four sampling were carried out: two in the summer (rainy season) and two in winter (dry season). The whole sediment was assessed by acute and chronic toxicity tests with Hyalella azteca and Ceriodaphnia dubia, respectively, the elutriate was assessed by chronic toxicity test using C. dubia, while the porewater was evaluated by acute toxicity test with Vibrio fischeri. Samples of river water were also evaluated for chronic toxicity tests with C. dubia. The quantification of metals and hydrocarbons in sediment samples was also carried out in order to correlate the biological effects with the chemical contamination. The obtained results with the whole sediment test indicate Mogi das Cruzes and Suzano cities as the most toxic sites and also as the sites with the highest

Screening and identification of sites for a proposed Monitored Retrievable Storage Facility

International Nuclear Information System (INIS)

1985-04-01

The Director, Office of Civilian Radioactive Waste Management (OCRWM), Department of Energy (DOE), has identified the Clinch River Breeder Reactor site, the DOE Oak Ridge Reservation and the Tennessee Valley Authority (TVA) Hartsville Nuclear Plant site as preferred and alternative sites, respectively, for development of site-specific designs as part of the proposal for construction of an integrated Monitored Retrievable Storage (MRS) Facility. The proposal, developed pursuant to Section 141 (b) of the Nuclear Waste Policy Act of 1982, will be submitted to Congress in January 1986. The Director expects to propose to Congress that an MRS be constructed at the perferred site. His judgment could change based on information to be developed between now and January 1986. The decision to construct an MRS facility and final site selection are reserved by Congress for itself. The Director's judgment is based on the results of a rigorous site screening and evaluation process described in this report. The three sites were selected from among eleven sites evaluated in detail. The Clinch River Breeder Reactor site, owned by the Tennessee Valley Authority, was identified as the preferred site. It has several particularly desirable features including: (1) federal ownership and control by the Department of Energy; (2) particularly good transportation access (five miles to the nearest interstate highway and direct rail access); (3) site characteristics and current data base judged by the NRC in 1983 as sufficient for granting a limited work authorization for the now cancelled breeder reactor; and (4) a technical community in the vicinity of site which can provide experienced nuclear facility support functions. 6 figs., 2 tabs

Radionuclide migration pathways analysis for the Oak Ridge Central Waste Disposal Facility on the West Chestnut Ridge site

International Nuclear Information System (INIS)

Pin, F.G.; Witherspoon, J.P.; Lee, D.W.; Cannon, J.B.; Ketelle, R.H.

1984-10-01

A dose-to-man pathways analysis is performed for disposal of low-level radioactive waste at the Central Waste Disposal Facility on the West Chestnut Ridge Site. Both shallow land burial (trench) and aboveground (tumulus) disposal methods are considered. The waste volumes, characteristics, and radionuclide concentrations are those of waste streams anticipated from the Oak Ridge National Laboratory, the Y-12 Plant, and the Oak Ridge Gaseous Diffusion Plant. The site capacity for the waste streams is determined on the basis of the pathways analysis. The exposure pathways examined include (1) migration and transport of leachate from the waste disposal units to the Clinch River (via the groundwater medium for trench disposal and Ish Creek for tumulus disposal) and (2) those potentially associated with inadvertent intrusion following a 100-year period of institutional control: an individual resides on the site, inhales suspended particles of contaminated dust, ingests vegetables grown on the plot, consumes contaminated water from either an on-site well or from a nearby surface stream, and receives direct exposure from the contaminated soil. It is found that either disposal method would provide effective containment and isolation for the anticipated waste inventory. However, the proposed trench disposal method would provide more effective containment than tumuli because of sorption of some radionuclides in the soil. Persons outside the site boundary would receive radiation doses well below regulatory limits if they were to ingest water from the Clinch River. An inadvertent intruder could receive doses that approach regulatory limits; however, the likelihood of such intrusions and subsequent exposures is remote. 33 references, 31 figures, 28 tables

Field sampling and analysis plan for the remedial investigation of Waste Area Grouping 2 at Oak Ridge National Laboratory, Oak Ridge, Tennessee

International Nuclear Information System (INIS)

Boston, H.L.; Ashwood, T.L.; Borders, D.M.; Chidambariah, V.; Downing, D.J.; Fontaine, T.A.; Ketelle, R.H.; Lee, S.Y.; Miller, D.E.; Moore, G.K.; Suter, G.W.; Tardiff, M.F.; Watts, J.A.; Wickliff, D.S.

1992-02-01

This field sampling and analysis (S ampersand A) plan has been developed as part of the Department of Energy's (DOE's) remedial investigation (RI) of Waste Area Grouping (WAG) 2 at Oak Ridge National Laboratory (ORNL) located in Oak Ridge, Tennessee. The S ampersand A plan has been written in support of the remedial investigation (RI) plan for WAG 2 (ORNL 1990). WAG 2 consists of White Oak Creek (WOC) and its tributaries downstream of the ORNL main plant area, White Oak Lake (WOL), White Oak Creek embayment (WOCE) on the Clinch River, and the associated floodplain and subsurface environment (Fig. 1.1). The WOC system is the surface drainage for the major ORNL WAGs and has been exposed to a diversity of contaminants from operations and waste disposal activities in the WOC watershed. WAG 2 acts as a conduit through which hydrologic fluxes carry contaminants from upgradient areas to the Clinch River. Water, sediment, soil, and biota in WAG 2 are contaminated and continue to receive contaminants from upgradient WAGs. This document describes the following: an overview of the RI plan, background information for the WAG 2 system, and objectives of the S ampersand A plan; the scope and implementation of the first 2 years of effort of the S ampersand A plan and includes recent information about contaminants of concern, organization of S ampersand A activities, interactions with other programs, and quality assurance specific to the S ampersand A activities; provides details of the field sampling plans for sediment, surface water, groundwater, and biota, respectively; and describes the sample tracking and records management plan

Aortic atresia with normal sized left ventricle

Directory of Open Access Journals (Sweden)

Priya Jagia

2016-01-01

Full Text Available Aortic atresia with an associated ventricular septal defect and adequate sized left ventricle is extremely rare. We present two cases in which an alternate diagnosis was suggested on echocardiography because the hypoplastic aortic trunk was missed due to its small caliber. The final diagnosis was, however, clinched on dual source computed tomography, which not only showed the thin aortic trunk but also clearly depicted the coronary artery origins from the hypoplastic aortic root. To the best of our knowledge, use of multi-detector computed tomography in aortic atresia with well developed left ventricle has not been reported in literature till date.

236-Z canyon utilization study

International Nuclear Information System (INIS)

Dixon, D.R.

1977-01-01

The 236-Z canyon contains equipment for repurification of plutonium and recovery of plutonium from scrap material. To meet production requirements of Fast Flux Test Facility/Clinch River Breeder Reactor oxide with the existing plant, several new pieces of equipment will be needed in the future. More storage space and a better accountability system are needed to support this increased production. The available canyon space needs to be utilized to its fullest in order to accommodate the new equipment. The purpose of this document is to identify the new pieces of equipment, show how they fit into the flowsheet, and locate them in the canyon

Safety approach to the selection of design criteria for the CRBRP reactor refueling system

International Nuclear Information System (INIS)

Meisl, C.J.; Berg, G.E.; Sharkey, N.F.

1979-01-01

The selection of safety design criteria for Liquid Metal Fast Breeder Reactor (LMFBR) refueling systems required the extrapolation of regulations and guidelines intended for Light Water Reactor refueling systems and was encumbered by the lack of benefit from a commercially licensed predecessor other than Fermi. The overall approach and underlying logic are described for developing safety design criteria for the reactor refueling system (RRS) of the Clinch River Breeder Reactor Plant (CRBRP). The complete selection process used to establish the criteria is presented, from the definition of safety functions to the finalization of safety design criteria in the appropriate documents. The process steps are illustrated by examples

LMFBR fuel analysis. Task A: Oxide fuel dynamics. Final report, October 1, 1976--September 30, 1977

International Nuclear Information System (INIS)

Dhir, V.K.; Doshi, J.; Frank, M.; Hauss, B.; Kastenberg, W.E.; Wong, K.

1977-10-01

The study presented deals with several areas of uncertainty in the analysis of the unprotected overpower transient for the Clinch River Breeder Reactor. These areas of uncertainty include the time, place, and mode of fuel pin failure; pre-failure fuel motion; fuel freezing, plugging, and plate-out following pin failure; and the potential for re-criticality. Internal molten fuel motion prior to pin failure was found to be sensitive to ramp rate and burnup. The strain-limit fuel failure criterion was found to be inappropriate for analysis based on existing data. The coupling of pre-transient- and transient-induced stresses tended to force the failure location towards the core midplane

Ecological risk assessment in a large river-reservoir. 1: Introduction and background

International Nuclear Information System (INIS)

Cook, R.B.; Suter, G.W. II; Sain, E.R.

1999-01-01

The US Department of Energy initiated a remedial investigation of the Clinch River/Poplar Creek system Superfund Site in 1989. This site, located in eastern Tennessee near Oak Ridge, consists of 70 river kilometers and 40 km 2 of surface area. The purpose of this study was to evaluate the nature and extent of contamination, perform an ecological and human health risk assessment, and evaluate possible remedial alternatives. This introductory article summarizes the environmental setting, the contamination history, and the study approach and provides some general results of the site characterization. Subsequent papers in this series describe the ecological risks to fish, piscivorous and insectivorous wildlife, and benthic invertebrates

Case for integral core-disruptive accident analysis

International Nuclear Information System (INIS)

Luck, L.B.; Bell, C.R.

1985-01-01

Integral analysis is an approach used at the Los Alamos National Laboratory to cope with the broad multiplicity of accident paths and complex phenomena that characterize the transition phase of core-disruptive accident progression in a liquid-metal-cooled fast breeder reactor. The approach is based on the combination of a reference calculation, which is intended to represent a band of similar accident paths, and associated system- and separate-effect studies, which are designed to determine the effect of uncertainties. Results are interpreted in the context of a probabilistic framework. The approach was applied successfully in two studies; illustrations from the Clinch River Breeder Reactor licensing assessment are included

Dynamic transient analysis of rupture disks by the finite-element method

International Nuclear Information System (INIS)

Hsieh, B.J.

1975-02-01

A finite element method utilizing the principle of virtual work in convected coordinates is used to analyze the axisymmetric dynamic transient response of rupture disks. This method can treat non-linearities arising both from inelastic material properties and large displacements/rotations provided that the convected strains are small. This report contains extensive calculations using a variety of rupture disk geometries and attempts to relate the static buckling of such disks to their dynamic response characteristics. A majority of the calculations treat the response of 18 inch disks typical of those currently considered for use in the Clinch River Breeder Reactor intermediate heat transport system

U.S. Department of Energy & Nuclear Regulatory Commission Advanced Fuel Cycle Research & Development Seminar Series FY 2007 & 2008

Energy Technology Data Exchange (ETDEWEB)

Grandy, Christopher [Argonne National Lab. (ANL), Argonne, IL (United States)

2008-08-01

In fiscal year 2007, the Advanced Burner Reactor project initiated an educational seminar series for the Department of Energy (DOE) and Nuclear Regulatory Commission (NRC) personnel on various aspects of fast reactor fuel cycle closure technologies. This important work was initiated to inform DOE and NRC personnel on initial details of sodium-cooled fast reactor, separations, waste form, and safeguard technologies being considered for the Advanced Fuel Cycle Research and Development program, and to learn the important lesson from the licensing process for the Clinch River Breeder Reactor Plant that educating the NRC staff early in the regulatory process is very important and critical to a project success.

Radiation protection/shield design

International Nuclear Information System (INIS)

Disney, R.K.

1977-01-01

Radiation protection/shielding design of a nuclear facility requires a coordinated effort of many engineering disciplines to meet the requirements imposed by regulations. In the following discussion, the system approach to Clinch River Breeder Reactor Plant (CRBRP) radiation protection will be described, and the program developed to implement this approach will be defined. In addition, the principal shielding design problems of LMFBR nuclear reactor systems will be discussed in realtion to LWR nuclear reactor system shielding designs. The methodology used to analyze these problems in the U.S. LMFBR program, the resultant design solutions, and the experimental verification of these designs and/or methods will be discussed. (orig.) [de

Liquid Metal Fast Breeder Reactor plant maintenance and equipment design

International Nuclear Information System (INIS)

Swannack, D.L.

1982-01-01

This paper provides a summary of maintenance equipment considerations and actual plant handling experiences from operation of a sodium-cooled reactor, the Fast Flux Test Facility (FFTF). Equipment areas relating to design, repair techniques, in-cell handling, logistics and facility services are discussed. Plant design must make provisions for handling and replacement of components within containment or allow for transport to an ex-containment area for repair. The modular cask assemblies and transporter systems developed for FFTF can service major plant components as well as smaller units. The plant and equipment designs for the Clinch River Breeder Reactor (CRBR) plant have been patterned after successful FFTF equipment

Design considerations for CRBRP heat transport system piping operating at elevated temperatures

International Nuclear Information System (INIS)

Pollono, L.P.; Mello, R.M.

1979-01-01

The heat transport system sodium piping for the Clinch River Breeder Reactor Plant (CRBRP) within the reactor containment building must withstand high temperatures for long periods of time. Each phase of the mechanical design process of the piping system is influenced by elevated temperature considerations which include material thermal creep effects, ratchetting caused by rapid temperature transients and stress relaxation, and material degradation effects. The structural design philosophy taken to design the CRBRP piping operating in a high temperature environment is described. The resulting design of the heat transport system piping is presented along with a discussion of special features that resulted from the elevated temperature considerations

Consolidation in a child from tuberculosis endemic area - thinking apart from tuberculosis

Directory of Open Access Journals (Sweden)

George P Jacob

2013-01-01

Full Text Available Tuberculosis is endemic in South East Asian regions and hence a disease commonly over diagnosed in these parts of the country. Patients presenting with chest x ray shadows and vague symptoms are often started on Anti Tuberculosis Treatment - smear negative. However caution should be administered in prescribing ATT to patients who do not improve symptomatically, even after intensive phase. Congenital abnormalities such as sequestrated lung at times can also be rare causes of abnormal skiagram chest, especially in paediatric patients. A careful systematic approach with non invasive imaging such as CECT chest will often help to clinch the diagnosis in most cases.

An empirical comparison of effective concentration estimators for evaluating aquatic toxicity test responses

Energy Technology Data Exchange (ETDEWEB)

Bailer, A.J.; Hughes, M.R.; Denton, D.L.; Oris, J.T.

2000-01-01

Aquatic toxicity tests are statistically evaluated by either hypothesis testing procedures to derive a no-observed-effect concentration or by inverting regression models to calculate the concentration associated with a specific reduction from the control response. These latter methods can be described as potency estimation methods. Standard US Environmental Protection Agency (USEPA) potency estimation methods are based on two different techniques. For continuous or count response data, a nominally nonparametric method that assumes monotonic decreasing responses and piecewise linear patterns between successive concentration groups is used. For quantal responses, a probit regression model with a linear dose term is fit. These techniques were compared with a recently developed parametric regression-based estimator, the relative inhibition estimator, RIp. This method is based on fitting generalized linear models, followed by estimation of the concentration associated with a particular decrement relative to control responses. These estimators, with levels of inhibition (p) of 25 and 50%, were applied to a series of chronic toxicity tests in a US EPA region 9 database of reference toxicity tests. Biological responses evaluated in these toxicity tests included the number of young produced in three broods by the water flea (Ceriodaphnia dubia) and germination success and tube length data from the giant kelp (Macrocystis pyrifera). The greatest discrepancy between the RIp and standard US EPA estimators was observed for C. dubia. The concentration-response pattern for this biological endpoint exhibited nonmonotonicity more frequently than for any of the other endpoint. Future work should consider optimal experimental designs to estimate these quantities, methods for constructing confidence intervals, and simulation studies to explore the behavior of these estimators under known conditions.

The long-term effects of acute exposure to ionising radiation on survival and fertility in Daphnia magna

International Nuclear Information System (INIS)

Sarapultseva, Elena I.; Dubrova, Yuri E.

2016-01-01

The results of recent studies have provided strong evidence for the transgenerational effects of parental exposure to ionising radiation and chemical mutagens. However, the transgenerational effects of parental exposure on survival and fertility remain poorly understood. To establish whether parental irradiation can affect the survival and fertility of directly exposed organisms and their offspring, crustacean Daphnia magna were given 10, 100, 1000 and 10,000 mGy of acute γ-rays. Exposure to 1000 and 10,000 mGy significantly compromised the viability of irradiated Daphnia and their first-generation progeny, but did not affect the second-generation progeny. The fertility of F 0 and F 1 Daphnia gradually declined with the dose of parental exposure and significantly decreased at dose of 100 mGy and at higher doses. The effects of parental irradiation on the number of broods were only observed among the F 0 Daphnia exposed to 1000 and 10,000 mGy, whereas the brood size was equally affected in the two consecutive generations. In contrast, the F 2 total fertility was compromised only among progeny of parents that received the highest dose of 10,000 mGy. We propose that the decreased fertility observed among the F 2 progeny of parents exposed to 10,000 mGy is attributed to transgenerational effects of parental irradiation. Our results also indicate a substantial recovery of the F 2 progeny of irradiated F 0 Daphnia exposed to the lower doses of acute γ-rays. - Highlights: • Viability of irradiated daphnids and their F 1 progeny is compromised. • Viability of the F 2 progeny of irradiated parents is not affected. • Total fertility of irradiated daphnids and their F 1 progeny declines with the dose. • Total fertility of the F 2 progeny of parents exposed to 10,000 mGy is compromised. • The decreased fertility among the F 2 progeny is transgenerational phenomenon.

Nickel and binary metal mixture responses in Daphnia magna: Molecular fingerprints and (sub)organismal effects

International Nuclear Information System (INIS)

Vandenbrouck, Tine; Soetaert, Anneleen; Ven, Karlijn van der; Blust, Ronny; Coen, Wim de

2009-01-01

The recent development of a custom cDNA microarray platform for one of the standard organisms in aquatic toxicology, Daphnia magna, opened up new ways to mechanistic insights of toxicological responses. In this study, the mRNA expression of several genes and (sub)organismal responses (Cellular Energy Allocation, growth) were assayed after short-term waterborne metal exposure. Microarray analysis of Ni-exposed daphnids revealed several affected functional gene classes, of which the largest ones were involved in different metabolic processes (mainly protein and chitin related processes), cuticula turnover, transport and signal transduction. Furthermore, transcription of genes involved in oxygen transport and heme metabolism (haemoglobin, δ-aminolevilunate synthase) was down-regulated. Applying a Partial Least Squares regression on nickel fingerprints and biochemical (sub)organismal parameters revealed a set of co-varying genes (haemoglobin, RNA terminal phosphate cyclase, a ribosomal protein and an 'unknown' gene fragment). An inverse relationship was seen between the mRNA expression levels of different cuticula proteins and available energy reserves. In addition to the nickel exposure, daphnids were exposed to binary mixtures of nickel and cadmium or nickel and lead. Using multivariate analysis techniques, the mixture mRNA expression fingerprints (Ni 2+ + Cd 2+ , Ni 2+ + Pb 2+ ) were compared to those of the single metal treatments (Ni 2+ , Cd 2+ , Pb 2+ ). It was hypothesized that the molecular fingerprints of the mixtures would be additive combinations of the gene transcription profiles of the individual compounds present in the mixture. However, our results clearly showed additionally affected pathways after mixture treatment (e.g. additional affected genes involved in carbohydrate catabolic processes and proteolysis), indicating interactive molecular responses which are not merely the additive sum of the individual metals. These findings, although indicative of

The long-term effects of acute exposure to ionising radiation on survival and fertility in Daphnia magna

Energy Technology Data Exchange (ETDEWEB)

Sarapultseva, Elena I. [Department of Biology, Institute of Nuclear Power Engineering NRNU MEPhI, Studgorodok,1, Obninsk, Kaluga Region 249040 (Russian Federation); National Research Nuclear University “MEPhI”, Kashirskoe Highway, 31, Moscow 115409 (Russian Federation); Dubrova, Yuri E., E-mail: yed2@le.ac.uk [Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH (United Kingdom); Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina Str. 3, 11933 Moscow (Russian Federation)

2016-10-15

The results of recent studies have provided strong evidence for the transgenerational effects of parental exposure to ionising radiation and chemical mutagens. However, the transgenerational effects of parental exposure on survival and fertility remain poorly understood. To establish whether parental irradiation can affect the survival and fertility of directly exposed organisms and their offspring, crustacean Daphnia magna were given 10, 100, 1000 and 10,000 mGy of acute γ-rays. Exposure to 1000 and 10,000 mGy significantly compromised the viability of irradiated Daphnia and their first-generation progeny, but did not affect the second-generation progeny. The fertility of F{sub 0} and F{sub 1}Daphnia gradually declined with the dose of parental exposure and significantly decreased at dose of 100 mGy and at higher doses. The effects of parental irradiation on the number of broods were only observed among the F{sub 0}Daphnia exposed to 1000 and 10,000 mGy, whereas the brood size was equally affected in the two consecutive generations. In contrast, the F{sub 2} total fertility was compromised only among progeny of parents that received the highest dose of 10,000 mGy. We propose that the decreased fertility observed among the F{sub 2} progeny of parents exposed to 10,000 mGy is attributed to transgenerational effects of parental irradiation. Our results also indicate a substantial recovery of the F{sub 2} progeny of irradiated F{sub 0}Daphnia exposed to the lower doses of acute γ-rays. - Highlights: • Viability of irradiated daphnids and their F{sub 1} progeny is compromised. • Viability of the F{sub 2} progeny of irradiated parents is not affected. • Total fertility of irradiated daphnids and their F{sub 1} progeny declines with the dose. • Total fertility of the F{sub 2} progeny of parents exposed to 10,000 mGy is compromised. • The decreased fertility among the F{sub 2} progeny is transgenerational phenomenon.

The combined effect of bottom-up and top-down factors on life history and reproduction of Daphnia in the field: is a strategic dilemma underlying population declines?

Directory of Open Access Journals (Sweden)

Stephan HÜLSMANN

2011-08-01

Full Text Available In a detailed field study covering three years, population dynamics, life history shifts and reproductive patterns of a population of Daphnia galeata were investigated in relation to food availability and dynamics of young of the year fish, the main vertebrate predators. In all years an increase of Daphnia abundance in spring was associated with declining food conditions (clear water stage. The size at maturity (SAM during this period was high and even increased, brood size declined, while egg volume increased. These patterns may be explained as response to food limitation. A decline of Daphnia abundance in every year was associated with increasing food conditions and the end of the clear water stage. Egg volumes as well as the size of egg-carrying daphnids also decreased, while brood size remained at low values. In two years in which these changes were particularly pronounced, the available fish data suggest that both the biomass (determining predation pressure, as well as the size of the fish (determining size selection are important for the observed dynamics. No decrease of SAM occurred as long as fish were smaller than 25 mm TL, when they are still gape-limited and not able to feed on the largest size-classes of Daphnia. Although fish biomass, which should correspond to kairomone level, would have suggested a reduction of SAM as induced defence, probably the selection of small (egg-carrying daphnids by small fish, besides severe food limitation, prevented this response to become effective. The sudden decline of SAM at the end of the clear water stage may not be explained by gradual phenotypic responses or indirect demographic effects, but must be due to an alternation of generations. SAM may be further reduced by direct and indirect effects of predation, interacting with increasing food levels. The Daphnia population is most vulnerable to predation at the time when the new generation takes over. Consequently, predation impact depends on

Relationships determining the toxicity of major ion mixtures to Ceriodaphnia dubia

Science.gov (United States)

Significant impacts to aquatic systems can occur due to increases in major ions (Na, K, Ca, Mg, Cl, SO4, HCO3) from various anthropogenic activities, these impacts varying with both the specific combination of ions that are elevated and the chemistry of the background water. A s...

Antibiotics in the surface water of the Yangtze Estuary: occurrence, distribution and risk assessment.

Science.gov (United States)

Yan, Caixia; Yang, Yi; Zhou, Junliang; Liu, Min; Nie, Minghua; Shi, Hao; Gu, Lijun

2013-04-01

The occurrence and distribution of five groups of antibiotics were investigated in the surface water of Yangtze Estuary over four seasons. Of the 20 antibiotics, only sulfamerazine was not detected at all sampling sites, indicating widespread occurrence of antibiotic residues in the study area. Detection frequencies and concentrations of antibiotics were generally higher in January, indicating that low flow conditions and low temperature might enhance the persistence of antibiotics in water. Antibiotic levels varied with location, with the highest concentrations being observed around river discharge and sewage outfall. Furthermore, a positive correlation between total antibiotic and DOC concentrations revealed the significant role played by DOC. Risk assessment based on single compound exposure showed that sulfapyridine and sulfamethoxazole could cause medium risk to daphnid in the Yangtze Estuary. Copyright © 2012 Elsevier Ltd. All rights reserved.

Industrial safety and applied health physics. Annual report for 1980

International Nuclear Information System (INIS)

1981-11-01

Information is reported in sections entitled: radiation monitoring; Environmental Management Program; radiation and safety surveys; industrial safety and special projects; Office of Operational Safety; and training, lectures, publications, and professional activities. There were no external or internal exposures to personnel which exceeded the standards for radiation protection as defined in DOE Manual Chapter 0524. Only 35 employees received whole body dose equivalents of 10 mSv (1 rem) or greater. There were no releases of gaseous waste from the Laboratory which were of a level that required an incident report to DOE. There were no releases of liquid radioactive waste from the Laboratory which were of a level that required an incident report to DOE. The quantity of those radionuclides of primary concern in the Clinch River, based on the concentration measured at White Oak Dam and the dilution afforded by the Clinch River, averaged 0.16 percent of the concentration guide. The average background level at the Perimeter Air Monitoring (PAM) stations during 1980 was 9.0 μrad/h (0.090 μGy/h). Soil samples were collected at all perimeter and remote monitoring stations and analyzed for eleven radionuclides including plutonium and uranium. Plutonium-239 content ranged from 0.37 Bq/kg (0.01 pCi/g) to 1.5 Bq/kg (0.04 pCi/g), and the uranium-235 content ranged from 0.7 Bq/kg (0.02 pCi/g) to 16 Bq/kg (0.43 pCi/g). Grass samples were collected at all perimeter and remote monitoring stations and analyzed for twelve radionuclides including plutonium and uranium. Plutonium-239 content ranged from 0.04 Bq/kg (0.001 pCi/g) to 0.07 Bq/kg (0.002 pCi/g), and the uranium-235 content ranged from 0.37 Bq/kg (0.01 pCi/g) to 12 Bq/kg

Waste Area Grouping 2 Remedial Investigation Phase 1 Seep Task data report: Contaminant source area assessment

International Nuclear Information System (INIS)

Hicks, D.S.

1996-03-01

This report presents the findings of the Waste Area Grouping (WAG) 2, Phase 1 Remedial Investigation (RI) Seep Task efforts during 1993 and 1994 at Oak Ridge National Laboratory (ORNL). The results presented here follow results form the first year of sampling, 1992, which are contained in the Phase 1 RI report for WAG 2 (DOE 1995a). The WAG 2 Seep Task efforts focused on contaminants in seeps, tributaries, and main streams within the White Oak Creek (WOC) watershed. This report is designed primarily as a reference for contaminants and a resource for guiding remedial decisions. Additional in-depth assessments of the Seep Task data may provide clearer understandings of contaminant transport from the different source areas in the WOC watershed. WAG 2 consists of WOC and its tributaries downstream of the ORNL main plant area, White Oak Lake, the White Oak Creek Embayment of the Clinch River, and the associated flood plains and subsurface environment. The WOC watershed encompasses ORNL and associated WAGs. WAG 2 acts as an integrator for contaminant releases from the contaminated sites at ORNL and as the conduit transporting contaminants to the Clinch River. The main objectives of the Seep Task were to identify and characterize seeps, tributaries and source areas that are responsible for the contaminant releases to the main streams in WAG 2 and to quantify their input to the total contaminant release from the watershed at White Oak Dam (WOD). Efforts focused on 90 Sr, 3 H, and 137 Cs because these contaminants pose the greatest potential human health risk from water ingestion at WOD. Bimonthly sampling was conducted throughout the WOC watershed beginning in March 1993 and ending in August 1994. Samples were also collected for metals, anions, alkalinity, organics, and other radionuclides

Human behaviours associated with dominance in elite amateur boxing bouts: A comparison of winners and losers under the Ten Point Must System.

Science.gov (United States)

Dunn, Emily C; Humberstone, Clare E; Iredale, K Fiona; Martin, David T; Blazevich, Anthony J

2017-01-01

Humans commonly ascertain physical dominance through non-lethal fighting by participating in combat sports. However, the behaviours that achieve fight dominance are not fully understood. Amateur boxing competition, which is judged using the subjective "Ten Point Must-System", provides insight into fight dominance behaviours. Notational analysis was performed on 26 elite male competitors in a national boxing championship. Behavioural (guard-drop time; movement style [stepping/bouncing time]; clinch-time; interaction-time) and technical (total punches; punches landed [%Hit]; air punches [%Air]; defence) measures were recorded. Participants reported effort required (0-100%) and perceived effect of fatigue on their own performance (5-point Likert scale) following bouts. Differences between winners and losers, and changes across the duration of the bout were examined. Winners punched more accurately than losers (greater %Hit [33% vs. 23%] and lower %Air [17% vs. 27%]) but total punches, defence and interaction-time were similar. From rounds 1-2, clinch-time and guard drops increased whilst bouncing decreased. Perceived effect of fatigue increased throughout the bout while perceived effort increased only from rounds 2-3. %Hit and movement index together in regression analysis correctly classified 85% of bout outcomes, indicating that judges (subjectively) chose winning (dominant) boxers according to punch accuracy and style, rather than assertiveness (more punches thrown). Boxers appear to use tactical strategies throughout the bout to pace their effort and minimise fatigue (increased guard drops, reduced bouncing), but these did not influence perceived dominance or bout outcome. These results show that judges use several performance indicators not including the total number of successful punches thrown to assess fight dominance and superiority between fighters. These results provide valuable information as to how experienced fight observers subjectively rate superiority

The Role of Highly Unsaturated Fatty Acids in Aquatic Food Webs

Science.gov (United States)

Perhar, G.; Arhonditsis, G. B.

2009-05-01

Highly unsaturated fatty acids (HUFAs) are important molecules transferred across the plant-animal interface in aquatic food webs. Defined here as carbon chains of length 18 (carbons) or more, with a double bond in the third (Omega 3) or sixth (Omega 6) bond from the methyl end, HUFAs are formed in primary producers (phytoplankton). With limited abilities to synthesize de novo, consumers and higher trophic organisms are required to obtain their HUFAs primarily from diet. Bioconversion of HUFAs from one form to another is in theory possible, as is synthesis via elongation and the transformation of a saturated to highly saturated fatty acid, but the enzymes required for these processes are absent in most species. HUFAs are hypothesized to be somatic growth limiting compounds for herbivorous zooplankton and have been shown to be critical for juvenile fish growth and wellbeing. Zooplankton tend to vary their fatty acid concentrations, collection strategies and utilization methods based on taxonomy, and various mechanisms have been suggested to account for these differences i.e., seasonal and nervous system hypotheses. Considering also the facts that copepods overwinter in an active state while daphnids overwinter as resting eggs, and that copepods tend to accumulate Docosahexaenoic acid (DHA) through collection and bioconversion, while daphnids focus on Eicosapentaenoic acid (EPA), one can link high DHA concentrations to active overwintering; but both EPA and DHA have similar melting points, putting DHA's cold weather adaptation abilities into question. Another characteristic setting copepods apart from daphnids is nervous system complexity: copepod axons are coated in thick myelin sheaths, permitting rapid neural processing, such as rapid prey attack and intelligent predator avoidance; DHA may be required for the proper functioning of copepod neurons. Recent modeling results have suggested food webs with high quality primary producers (species high in HUFAs, i

Tennessee health studies agreement. Annual report for year 5, January 1 - December 31, 1996

International Nuclear Information System (INIS)

1997-05-01

This report summarizes the Oak Ridge Health Studies project for the period January 1, 1996, to December 31, 1996. Attention is focused on dose reconstruction which is comprised of seven separate tasks. They are: Task 1, investigation of radioiodine from radioactive lanthanum processing; Task 2, investigation of mercury releases from lithium enrichment; Task 3, investigation of releases of PCBs from Oak Ridge facilities; Task 4, investigation of releases of radionuclides from White Oak Creek to the Clinch River; Task 5, plan to perform a systematic document search; Task 6, investigation of the quality of historical uranium effluent monitoring of Oak Ridge facilities; and Task 7, additional screening of materials not evaluated in the dose reconstruction feasibility study

Tennessee health studies agreement. Annual report for year 5, January 1--December 31, 1996

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-05-01

This report summarizes the Oak Ridge Health Studies project for the period January 1, 1996, to December 31, 1996. Attention is focused on dose reconstruction which is comprised of seven separate tasks. They are: Task 1, investigation of radioiodine from radioactive lanthanum processing; Task 2, investigation of mercury releases from lithium enrichment; Task 3, investigation of releases of PCBs from Oak Ridge facilities; Task 4, investigation of releases of radionuclides from White Oak Creek to the Clinch River; Task 5, plan to perform a systematic document search; Task 6, investigation of the quality of historical uranium effluent monitoring of Oak Ridge facilities; and Task 7, additional screening of materials not evaluated in the dose reconstruction feasibility study.

Oak Ridge Dose Reconstruction annual report for calendar year 1997

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-09-01

Calendar year 1997 was the third full year of work on the Oak Ridge Dose Reconstruction. Activities are summarized on the following individual project tasks: Task 1 -- Investigation of radioiodine releases from X-10 radioactive lanthanum processing; Task 2 -- Investigation of mercury releases from Y-12 lithium enrichment; Task 3 -- Investigation of PCBs in the environment near Oak Ridge; Task 4 -- Investigation of radionuclides released from White Oak Creek to the Clinch River; Task 5 -- Systematic searching of records repositories; Task 6 -- Evaluation of the quality of uranium monitoring data and a screening evaluation of potential off-site health risks; and Task 7 -- Performance of screening for additional materials not evaluated in the feasibility study.

Contaminated sediment transport during floods

International Nuclear Information System (INIS)

Fontaine, T.A.

1992-01-01

Over the past 48 years, operations and waste disposal activities at Oak Ridge National Laboratory have resulted in the contamination of parts of the White Oak Creek catchment. The contaminants presenting the highest risk to human health and the environment are particle reactive and are associated with the soils and sediments in the White Oak Creek drainage system. The erosion of these sediments during floods can result in the transport of contaminants both within the catchment and off-site into the Clinch River. A data collection program and a modeling investigation are being used to evaluate the probability of contaminated sediment transport during floods and to develop strategies for controlling off-site transport under present and future conditions

BRENDA: a dynamic simulator for a sodium-cooled fast reactor power plant

International Nuclear Information System (INIS)

Hetrick, D.L.; Sowers, G.W.

1978-06-01

This report is a users' manual for one version of BRENDA (Breeder Reactor Nuclear Dynamic Analysis), which is a digital program for simulating the dynamic behavior of a sodium-cooled fast reactor power plant. This version, which contains 57 differential equations, represents a simplified model of the Clinch River Breeder Reactor Project (CRBRP). BRENDA is an input deck for DARE P (Differential Analyzer Replacement, Portable), which is a continuous-system simulation language developed at the University of Arizona. This report contains brief descriptions of DARE P and BRENDA, instructions for using BRENDA in conjunction with DARE P, and some sample output. A list of variable names and a listing for BRENDA are included as appendices

Feedback control systems for non-linear simulation of operational transients in LMFBRs

International Nuclear Information System (INIS)

Khatib-Rahbar, M.; Agrawal, A.K.; Srinivasan, E.S.

Adequate modeling of Plant Control Systems (PCS) for the study of Anticipated Transients Without Scram (ATWS) is of considerable significance in the design, operation and safety evaluation of Liquid-Metal-Cooled Fast Breeder Reactor (LMFBR) systems. To assess the system response to high frequency, low consequence events, the plant needs to be dynamically simulated. The description of analytical and numerical models for PCS that have been developed and incorporated into the loop version of the Super System Code (SSC-L) are described. The importance of detailed modeling of control systems is discussed. Sample transient results obtained for a 10% ramp change of load in 40 s in the Clinch River Breeder Reactor Plant (CRBRP) are also shown

Medium-range containerships making much headway%中型集装箱船大行其道

Institute of Scientific and Technical Information of China (English)

宋洋

2005-01-01

In the first 5 months of 2004, the transaction of the containerships worldwide continued maintaining a strong momentum. Among them, those medium-sized and small-sized containerships under 3000 TUE were clinched, totaling 85 units, up to 2.1 million dwt. These particular type of containerships have enjoyed substantial appreciation in the markets for reselling both the second-handed ships and new buildings since the second half of 2000. According to the statistics from the ship leasing market, the leasing rate for the medium-sized containerships has already topped its past records popped up in the third quarter of 2000. Likewise, the pricing of the small-and medium-sized containerships to be ordered has also exceeded its record high.

LMFBR fuel analysis. Task B. Post-accident heat removal. Final report, July 1, 1975--September 30, 1976

International Nuclear Information System (INIS)

Castle, J.; Catton, I.; Somerton, C.; Wu, R.

1976-11-01

The report deals with the behavior of molten core debris following a hypothetical core disruptive accident in the proposed Clinch River Breeder Reactor Plant. Heat dissipating characteristics of an ex-vessel sacrificial bed have been analyzed. A novel form of heat transfer, analogous to film boiling, has been proposed to describe heat transfer from a heat generating pool to surrounding steel walls. Bounding type heat transfer calculations are also made to quantify such hypothetical accident characteristics as debris bed remelting, debris bed dryout in sodium, and failure of the reactor cavity steel liner. Several documents that have been submitted to the NRC for its review of the CRBRP are discussed with attention being drawn to heat transfer related issues

Nuclear policy just rhetoric, says Gilinsky

International Nuclear Information System (INIS)

Anon.

1981-01-01

Mr. Gilinsky, a member of the Nuclear Regulatory Commission, labels administration nuclear policy as empty rhetoric that will not address the nuclear industry's real problems of the decline of nuclear power. He projects that another 20 plants under construction will join the 11 that were already cancelled or abandoned unless the economic situation changes. He argues that streamlining licensing not only won't help but will introduce a conflict of interest problem as the plan was presented. The real problem goes back to utilities entering the nuclear business without understanding the dangers and technical problems or having the managerial competence. Commercial reprocessing will not occur if federal money is withdrawn, making the Clinch River Breeder Project unnecessary

Prototype spent-fuel canister design, analysis, and test

International Nuclear Information System (INIS)

Leisher, W.B.; Eakes, R.G.; Duffey, T.A.

1982-03-01

Sandia National Laboratories was asked by the US Energy Research and Development Administration (now US Department of Energy) to design the spent fuel shipping cask system for the Clinch River Breeder Reactor Plant (CRBRP). As a part of this task, a canister which holds liquid sodium and the spent fuel assembly was designed, analyzed, and tested. The canister body survived the regulatory Type-B 9.1-m (30-ft) drop test with no apparent leakage. However, the commercially available metal seal used in this design leaked after the tests. This report describes the design approach, analysis, and prototype canister testing. Recommended work for completing the design, when funding is available, is included

Electromagnetic linear pump for liquid-metal service

International Nuclear Information System (INIS)

Meisner, J.

Electromagnetic pumps are not noted for their efficiency - values on the order of 15% are not unusual. However, the Energy Systems Group of Rockwell International, under contract to the Department of Energy, has recently designed, built, and tested for the Clinch River Breeder Reactor Program (CRBRP) a pump that has a peak efficiency of 40%. This outstanding efficiency was obtained by optimizing the hydraulic and electrical properties of the pumping section early in the design phase. These performance features were proven in a highly successful series of tests at flow rates up to 800 gal/min and temperatures up to 1130 0 F. This article discusses the design in detail and presents some of the test results

Oak Ridge Dose Reconstruction annual report for calendar year 1997

International Nuclear Information System (INIS)

1998-01-01

Calendar year 1997 was the third full year of work on the Oak Ridge Dose Reconstruction. Activities are summarized on the following individual project tasks: Task 1 -- Investigation of radioiodine releases from X-10 radioactive lanthanum processing; Task 2 -- Investigation of mercury releases from Y-12 lithium enrichment; Task 3 -- Investigation of PCBs in the environment near Oak Ridge; Task 4 -- Investigation of radionuclides released from White Oak Creek to the Clinch River; Task 5 -- Systematic searching of records repositories; Task 6 -- Evaluation of the quality of uranium monitoring data and a screening evaluation of potential off-site health risks; and Task 7 -- Performance of screening for additional materials not evaluated in the feasibility study

Anticipated transients without scram for light water reactors: implications for liquid metal fast breeder reactors

International Nuclear Information System (INIS)

Kastenberg, W.E.; Solomon, K.A.

1979-07-01

In the design of light water reactors (LWRs), protection against anticipated transients (e.g., loss of normal electric power and control rod withdrawal) is provided by a highly reliable scram, or shutdown system. If this system should become inoperable, however, the transient could lead to a core meltdown. The Nuclar Regulatory Commission (NRC) has proposed, in NUREG-0460 [1], new requirements (or acceptance criteria) for anticipated transients without scram (ATWS) events and the manner in which they could be considered in the design and safety evaluation of LWRs. This note assesses the potential impact of the proposed LWR-ATWS criteria on the liquid metal fast breeder reactor (LMFBR) safety program as represented by the Clinch River Breeder Reactor Plant

Integrated assessment of the impacts of agricultural drainwater in the Salinas River (California, USA)

Energy Technology Data Exchange (ETDEWEB)

Anderson, B.S.; Hunt, J.W.; Phillips, B.M.; Nicely, P.A.; Vlaming, V. de; Connor, V.; Richard, N.; Tjeerdema, R.S

2003-08-01

Invertebrate mortality was correlated with levels of water and sediment contaminatioin in the Salinas River. - The Salinas River is the largest of the three rivers that drain into the Monterey Bay National Marine Sanctuary in central California. Large areas of this watershed are cultivated year-round in row crops and previous laboratory studies have demonstrated that acute toxicity of agricultural drainwater to Ceriodaphnia dubia is caused by the organophosphate (OP) pesticides chlorpyrifos and diazinon. In the current study, we used a combination of ecotoxicologic tools to investigate incidence of chemical contamination and toxicity in waters and sediments in the river downstream of a previously uncharacterized agricultural drainage creek system. Water column toxicity was investigated using a cladoceran C. dubia while sediment toxicity was investigated using an amphipod Hyalella azteca. Ecological impacts of drainwater were investigated using bioassessments of macroinvertebrate community structure. The results indicated that Salinas River water downstream of the agricultural drain is acutely toxic to Ceriodaphnia, and toxicity to this species was highly correlated with combined toxic units (TUs) of chlorpyrifos and diazinon. Laboratory tests were used to demonstrate that sediments in this system were acutely toxic to H. azteca, which is a resident genus. Macroinvertebrate community structure was moderately impacted downstream of the agricultural drain input. While the lowest macroinvertebrate abundances were measured at the station demonstrating the greatest water column and sediment toxicity and the highest concentrations of pesticides, macroinvertebrate metrics were more significantly correlated with bank vegetation cover than any other variable. Results of this study suggest that pesticide pollution is the likely cause of laboratory-measured toxicity in the Salinas River samples and that this factor may interact with other factors to impact the

Paratrygon aiereba (Müller & Henle, 1841: the senior synonym of the freshwater stingray disceus thayeri Garman, 1913 (chondrichthyes: potamotrygonidae

Directory of Open Access Journals (Sweden)

Ricardo S. Rosa

1990-01-01

Full Text Available A review of the original descriptions and available types of the South American freshwater stingrays Trygon aiereba Müller & Henle, 1841, Trygon strogylopterus Schomburgk, 1843 and Disceus thayeri Garman, 1913 indicates that these three nominal species are synonymous. The senior name, placed in the monotypic genus Paratrygon Duméril, 1865, established for Trygon aiereba, is available and valid. Based on its description, T. aiereba is a freshwater stingray. Therefore, this taxon is not the same as Raja ajereba Walbaum and Raja orbicularis Schneider, both names established for the marine "Aiereba" of Marcgrave, and which preferably should be treated as nomina dubia. Paratrygon does not replace Potamotrygon Garman, 1877 as the type genus, because the family name Potamotrygonidae Garman, 1877 has priority over Paratrygonidade Gill, 1893.A revisão das descrições originais e dos tipos existentes das raias de água doce Trygon aiereba Müller & Henle, 1841, Trygon strogylopterus Schomburgk, 1843, e Disceus thayeri Garman, 1913, indica que essas três espécies nominais são sinônimas. O nome sênior, colocado no gênero monotípico Paratrygon Duméril, 1865, estabelecido para T. aiereba, é disponível e válido. A descrição de T. aiereba corresponde claramente a uma raia de agua doce; portanto esta espécie não é um sinônimo de Raja ajereba Walbaum e de Raja orbicularis Schneider, ambos os nomes propostos para a "Aiereba " marinha de Marcgrave, e que preferivelmente devem ser tratados como nomina dubia. Paratrygon não substitui Potamotrygon Garman, 1877 como gênero-tipo, porque o nome de família Potamotrygonidae Garman, 1877 tem prioridade sobre Paratrygonidae Gill. 1893.

Integrated assessment of the impacts of agricultural drainwater in the Salinas River (California, USA)

International Nuclear Information System (INIS)

Anderson, B.S.; Hunt, J.W.; Phillips, B.M.; Nicely, P.A.; Vlaming, V. de; Connor, V.; Richard, N.; Tjeerdema, R.S.

2003-01-01

Invertebrate mortality was correlated with levels of water and sediment contaminatioin in the Salinas River. - The Salinas River is the largest of the three rivers that drain into the Monterey Bay National Marine Sanctuary in central California. Large areas of this watershed are cultivated year-round in row crops and previous laboratory studies have demonstrated that acute toxicity of agricultural drainwater to Ceriodaphnia dubia is caused by the organophosphate (OP) pesticides chlorpyrifos and diazinon. In the current study, we used a combination of ecotoxicologic tools to investigate incidence of chemical contamination and toxicity in waters and sediments in the river downstream of a previously uncharacterized agricultural drainage creek system. Water column toxicity was investigated using a cladoceran C. dubia while sediment toxicity was investigated using an amphipod Hyalella azteca. Ecological impacts of drainwater were investigated using bioassessments of macroinvertebrate community structure. The results indicated that Salinas River water downstream of the agricultural drain is acutely toxic to Ceriodaphnia, and toxicity to this species was highly correlated with combined toxic units (TUs) of chlorpyrifos and diazinon. Laboratory tests were used to demonstrate that sediments in this system were acutely toxic to H. azteca, which is a resident genus. Macroinvertebrate community structure was moderately impacted downstream of the agricultural drain input. While the lowest macroinvertebrate abundances were measured at the station demonstrating the greatest water column and sediment toxicity and the highest concentrations of pesticides, macroinvertebrate metrics were more significantly correlated with bank vegetation cover than any other variable. Results of this study suggest that pesticide pollution is the likely cause of laboratory-measured toxicity in the Salinas River samples and that this factor may interact with other factors to impact the

Novas citações de Chytridiomycota e Oomycota para o Parque Estadual das Fontes do Ipiranga (PEFI, SP, Brasil New records of Chytridiomycota and Oomycota from the "Parque Estadual das Fontes do Ipiranga (PEFI", SP, Brazil

Directory of Open Access Journals (Sweden)

Carmen Lidia Amorim Pires-Zottarelli

2007-03-01

Full Text Available Levantamento de fungos zoospóricos realizado em lagos artificiais do Parque Estadual das Fontes do Ipiranga (PEFI, situado na cidade de São Paulo, Estado de São Paulo, Brasil, resultou no isolamento de 41 táxons, sendo 36 identificados em nível específico. Vinte e três táxons pertencem ao Reino Fungi, filo Chytridiomycota, ordens Chytridiales, Monoblepharidales e Spizellomicetales e, 18 ao Reino Straminipila, filo Oomycota, ordens Saprolegniales e Peronosporales. Dentre os fungos isolados, 14 são mencionados pela primeira vez para o PEFI: Aphanomyces helicoides von Minden, Catenochytridium kevorkianii Sparrow, Catenochytridium sp., Chytriomyces appendiculatus Karling, C. aureus Karling, C. hyalinus Karling, C. spinosus Fay, Diplophlyctis asteroidea Dogma, D. complicata (Willoughby Dogma, Karlingia dubia Karling, Nowakowskiella hemisphaerospora Shanor, Saprolegnia australis Elliott, Septochytrium variabile Berdan e Truittella sp., os quais são descritos, comentados e ilustrados. O gênero Truitella é mencionado pela primeira vez para o Brasil.A survey of zoosporic fungi from artificial lakes of the Fontes do Ipiranga State Park, in the city of São Paulo, São Paulo State, Brazil, yielded the isolation of 41 taxa with 36 species. Twenty-three taxa belong to Chytridiales, Monoblepharidales and Spizellomycetales of the Phylum Chytridiomycota, Fungi Kingdom and 18 belong to Saprolegniales and Peronosporales, Phylum Oomycota, Straminipila Kingdom. Of these, 14 are first-time records for this State Park: Aphanomyces helicoides von Minden, Catenochytridium kevorkianii Sparrow, Catenochytridium sp., Chytriomyces appendiculatus Karling, C. aureus Karling, C. hyalinus Karling, C. spinosus Fay, Diplophlyctis asteroidea Dogma, D. complicata (Willoughby Dogma, Karlingia dubia Karling, Nowakowskiella hemisphaerospora Shanor, Saprolegnia australis Elliott, Septochytrium variabile Berdan and Truittella sp., which are described, commented and

Design of the upper internals structure for the Clinch River Breeder Reactor Plant

International Nuclear Information System (INIS)

Thompson, D.C.; Novendstern, E.H.

1977-01-01

The Upper Internals Structure (UIS) is located above the core and is supported from the head at four locations. It is designed to perform the following primary functions: provide secondary core holddown in the event of a malfunction of the core hydraulic holddown system; provide support for routing all in-vessel instrumentation to core assemblies; maintain alignment between the core assemblies, the UIS and the closure head; provide guidance and crossflow protection for the control rod drivelines; and mix/duct flow to the upper region of the vessel outlet plenum to minimize rapid temperature changes to components during a reactor trip transient. In accomplishing these functions, the UIS will experience a sodium environment with temperatures up to 1200 0 F (649 0 C), and as many as 7 x 10 8 cycles of fluid temperature fluctuations up to 250 0 F (121 0 C) at full power operation. It must be designed to survive these conditions in combination with seismic and flow-induced vibration loadings for its 30 year design life. The design program of designing to controlled functional requirements and design conditions is discussed. Included is a description of the significant parts of the design and the approach used to balance the requirement of tight joints. The thermal and hydraulic environment including the results of a comprehensive test program are discussed. The test program results establish the basis of the thermal boundary used in the structural evaluation, and the UIS vibration characteristics. A summary of the areas which have required design changes is included with a summary of the structural evaluation of these changes

Empirical method to calculate Clinch River Breeder Reactor (CRBR) inlet plenum transient temperatures

International Nuclear Information System (INIS)

Howarth, W.L.

1976-01-01

Sodium flow enters the CRBR inlet plenum via three loops or inlets. An empirical equation was developed to calculate transient temperatures in the CRBR inlet plenum from known loop flows and temperatures. The constants in the empirical equation were derived from 1/4 scale Inlet Plenum Model tests using water as the test fluid. The sodium temperature distribution was simulated by an electrolyte. Step electrolyte transients at 100 percent model flow were used to calculate the equation constants. Step electrolyte runs at 50 percent and 10 percent flow confirmed that the constants were independent of flow. Also, a transient was tested which varied simultaneously flow rate and electrolyte. Agreement of the test results with the empirical equation results was good which verifies the empirical equation

Development and validation of OECD test guidelines on mollusc reproductive toxicity tests

DEFF Research Database (Denmark)

Lagadic, Laurent; Holbech, Henrik; hutchinson, tom

the comparison of endpoints relevant for reproduction in invertebrates often shows a much higher sensitivity in molluscs vs. e.g. daphnids. The OECD test guideline programme has thus been extended to cover reproduction effects of chemicals in molluscs. Existing mollusc toxicity test protocols have been reviewed...... in an OECD Detailed Review Paper that identifies two relevant candidate species for developing freshwater tests: Potamopyrgus antipodarum and Lymnaea stagnalis. However, this review did not clarify which toxicity test design/conditions are the most appropriate for chemicals assessment. Therefore, a mollusc...... reproduction test guideline will be developed describing partial- and full- life-cycle test protocols in these species, so as to propose a balanced suite of apical mollusc toxicity tests applicable for the assessment of any type of chemical, including endocrine disruptors, as level 4 and 5 assays of the EDTA...

Monitored retrievable storage submission to Congress: Volume 2, Environmental assessment for a monitored retrievable storage facility. [Contains glossary

Energy Technology Data Exchange (ETDEWEB)

None

1986-02-01

This Environmental Assessment (EA) supports the DOE proposal to Congress to construct and operate a facility for monitored retrievable storage (MRS) of spent fuel at a site on the Clinch River in the Roane County portion of Oak Ridge, Tennessee. The first part of this document is an assessment of the value of, need for, and feasibility of an MRS facility as an integral component of the waste management system. The second part is an assessment and comparison of the potential environmental impacts projected for each of six site-design combinations. The MRS facility would be centrally located with respect to existing reactors, and would receive and canister spent fuel in preparation for shipment to and disposal in a geologic repository. 207 refs., 57 figs., 132 tabs.

A manufacturer's view of the US breeder program

International Nuclear Information System (INIS)

Arnold, W.H.

1982-01-01

A liquid metal fast breeder reactor (LMFBR) was selected for development in a program to develop breeder reactors in general. The LMFBR is a sodium-cooled fast reactor which operates at a high conversion ratio of fertile-to-fissile material while generating electricity at a high thermal efficiency. The breeder has the added capacity to operate on the plutonium in Light Water Reactor spent fuel, and on U-238. A governmental/industrial infrastructure must be developed. Criteria for breeder deployment are listed. Construction of the Clinch River Breeder reactor is a necessary step in the progression to a mature breeder. Then the large prototype LMFBR should be built. Foreign collaboration is considered. Finally, a capital cost analysis indicates LMFBR cost-effectiveness

Monitored retrievable storage submission to Congress: Volume 2, Environmental assessment for a monitored retrievable storage facility

International Nuclear Information System (INIS)

1986-02-01

This Environmental Assessment (EA) supports the DOE proposal to Congress to construct and operate a facility for monitored retrievable storage (MRS) of spent fuel at a site on the Clinch River in the Roane County portion of Oak Ridge, Tennessee. The first part of this document is an assessment of the value of, need for, and feasibility of an MRS facility as an integral component of the waste management system. The second part is an assessment and comparison of the potential environmental impacts projected for each of six site-design combinations. The MRS facility would be centrally located with respect to existing reactors, and would receive and canister spent fuel in preparation for shipment to and disposal in a geologic repository. 207 refs., 57 figs., 132 tabs

Hydatid Cyst of Tongue: A Diagnostic Challenge

Directory of Open Access Journals (Sweden)

Neeraj Aggarwal

2017-12-01

Full Text Available Introduction Hydatid disease or human cystic echinococcosis is a parasitic zoonosis, endemic in the cattle and dog rearing region worldwide as well as in some parts of India.Cystic echinococcosis affects mostly the liver and lung (80%, but tongue is one of the very rare sites. Case Report A 10 year old boy had presented with an isolated cystic lesion in tongue, which was expelled spontaneously with no residual lesion. On detailed examination, no other site in the body was involved. Discussion Parasitic cyst of the tongue is rare entity. Microbiological and histopathological examination helped clinch the diagnosis Hydatid cyst should be considered as a differential diagnosis in isolated cystic lesion of tongue, especially in the risk group.

White Oak Creek embayment sediment retention structure: The Oak Ridge model in action

International Nuclear Information System (INIS)

Van Hoesen, S.D.; Kimmel, B.L.; Page, D.G.; Hudson, G.R.; Wilkerson, R.B.; Zocolla, M.

1992-01-01

White Oak Creek is the major surface-water drainage through the Department of Energy (DOE) Oak Ridge National Laboratory (ORNL). Samples taken from the lower portion of the creek revealed high levels of Cesium-137, and lower levels of Cobalt-60 in near-surface sediment. Other contaminants present in the sediment included: lead, mercury, chromium, and PCBS. In October 1990, DOE, US Environmental Protection Agency (EPA), and Tennessee Department of Environment and Conservation (DEC) agreed to initiate a time-critical removal action in accordance with Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) to prevent transport of the contaminated sediments into the Clinch River system. This paper discusses the environmental, regulatory, design, and construction issues that were encountered in conducting the remediation work

White Oak Creek embayment sediment retention structure design and construction

International Nuclear Information System (INIS)

Van Hoesen, S.D.; Kimmell, B.L.; Page, D.G.; Wilkerson, R.B.; Hudson, G.R.; Kauschinger, J.L.; Zocolla, M.

1994-01-01

White Oak Creek is the major surface water drainage throughout the Department of Energy (DOE) Oak Ridge National Laboratory (ORNL). Samples taken from the lower portion of the creek revealed high levels of Cesium 137 and lower level of Cobalt 60 in near surface sediment. Other contaminants present in the sediment included: lead, mercury, chromium, and PCBs. In October 1990, DOE, US Environmental Protection Agency (EPA), and Tennessee Department of Environment and Conservation (TDEC) agreed to initiate a time critical removal action in accordance with the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) to prevent the transport of the contaminated sediments into the Clinch River system. This paper discusses the environmental, regulatory, design, and construction issues that were encountered in conducting the remediation work

Statement of Ben C. Rusche, Director, Office of Civilian Radioactive Waste Management, Department of Energy

International Nuclear Information System (INIS)

Anon.

1987-01-01

The author appreciate the opportunity to appear before you today to discuss the Department of Energy's (DOE) proposal for Monitored Retrievable Storage (MRS). In our proposal, we recommend that Congress: Approve the construction of an MRS facility at Clinch River near Oak Ridge, Tennessee; Limit the storage capacity at the MRS facility to 15,000 metric tons of spent fuel; Preclude waste acceptance by the MRS facility until a construction authorization for the first repository is received from the NRC; Direct DOE to implement measures responsive to the concerns and recommendations of the State and local governments; and Direct DOE to implement the program plan accompanying the proposal. Before addressing several of the salient issues surrounding this proposal, I would like to provide relevant background information

Mechanical properties test program on structural materials in a sodium environment

International Nuclear Information System (INIS)

Natesan, K.; Chopra, O.K.; Kassner, T.F.

1979-10-01

This document describes in detail the ongoing and planned US Test program on the mechanical properties of sodium-exposed Type 316 austenitic stainless and Fe-2 1/4 Cr-1 Mo ferritic steels. The test program is based on the Development Requirement Specifications (DRS) established by the DOE/Clinch River Breeder Reactor Project (CRBRP) Program Office, the general need for the development of LMFBR structural-design criteria established by the Nuclear Systems Materials Handbook, and the need for a fundamental understanding of materials behavior in a sodium environment, which is generic to LMFBR systems. The planned test program is an extension of work based on current knowledge of sodium chemistry and the influence of sodium purity on the mechanical properties of structural materials

Renal disease masquerading as pyrexia of unknown origin

Directory of Open Access Journals (Sweden)

D Korivi

2013-01-01

Full Text Available Pyrexia of unknown origin is a challenging clinical problem. Infections, malignancies, and connective tissue diseases form the major etiologies for this condition. We report a case of a 57-year-old diabetic male who presented with fever of unknown origin for several months. The course of investigations led to a kidney biopsy which clinched the cause of his fever as well as the underlying diagnosis. The light microscopy findings of expansile storiform fibrosis with a dense inflammatory infiltrate suggested the diagnosis which was confirmed by positive staining of Immunoglobulin G4, the dense lympho-plasmacytic infiltrate and elevated serum IgG4 concentrations. A course of steroids followed by mycophenolate mofetil as maintenance immunosuppression rendered the patient afebrile with improvement of renal function.

Interlaboratory Comparison for Digestion Methods, Analytical Methods, and Holding Times for the Analysis of Trace Elements in Biological Samples for the Kingston Fly Ash Recovery Project

Energy Technology Data Exchange (ETDEWEB)

Mathews, Teresa J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Rogers, William J [Tennessee Valley Authority (TVA), Knoxville, TN (United States); Vitale, Rock [Environmental Standards, Inc., Knoxville, TN (United States); Smith, John G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Brandt, Craig C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Peterson, Mark J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Carriker, Neil [Tennessee Valley Authority (TVA), Knoxville, TN (United States)

2013-05-01

In December 2008, an ash dike at the Tennessee Valley Authority (TVA) Kingston Fossil Plant (KIF) ruptured, releasing over 1 billion gallons of coal ash into the Emory and Clinch Rivers. Coal ash may contain several contaminants of concern; of these, selenium (Se), arsenic (As), and mercury (Hg) have been highlighted in this work because of their toxicity and potential to bioaccumulate in aquatic food chains (Reash et al. 2006, Chapman et al. 2010). To assess the potential impact of the spilled coal ash on humans and the environment, a comprehensive monitoring program was established at the Kingston site, for which resident aquatic organisms (among other sample media) are collected to determine contaminant exposure and evaluate the risk to humans and wildlife.

Antioxidant and associated capacities of Camu camu (Myrciaria dubia): a systematic review.

Science.gov (United States)

Langley, Paul C; Pergolizzi, Joseph V; Taylor, Robert; Ridgway, Caroline

2015-01-01

An aging population in the United States presents important challenges for patients and physicians. The presence of inflammation can contribute to an accelerated aging process, the increasing presence of comorbidities, oxidative stress, and an increased prevalence of chronic pain. As patient-centered care is embracing a multimodal, integrative approach to the management of disease, patients and physicians are increasingly looking to the potential contribution of natural products. Camu camu, a well-researched and innovative natural product, has the potential to contribute, possibly substantially, to this management paradigm. The key issue is to raise camu camu's visibility through increased emphasis on its robust evidentiary base and its various formulations, as well as making consumers, patients, and physicians more aware of its potential. A program to increase the visibility of camu camu can contribute substantially not only to the management of inflammatory conditions and its positive contribution to overall good health but also to its potential role in many disease states.

Remarkable, overlooked and new microfungi in North Rhine-Westphalia, Germany.

Science.gov (United States)

Ale-Agha, N; Feige, G B; Linke, K

2001-01-01

During our investigations of the micro flora in NRW in the years of 1999 and 2000 we were able to collect and identify some new and rare species of micro fungi as parasites and saprophytes on wild and ornamental plants. Some of them are new for Germany: Podosphaera xanthii on Coreopsis verticillata; Cercospora traversiana on Trigonella foenum-graecum; Passalora dubia on Atriplex hortensis; Ophiobolus cirsii on Carduus spec.; Periconia britannica on Polemonium coeruleum; Ascochyta leptospora on Agropyron repens; Apomelasmia urticae on Urtica dioica; Cryptodiaporthe salicina on Salix caprea; Dasyscyphus nidulus on Anemone hupehensis; Rhopographus filicinus on Pteridium aquilinum; Sillia ferruginea on Corylus avellana; Sirococcus spiraeae on Spiraea spec. and Forsythia x intermedia. Examples of these findings are in the Herbarium ESS (Mycotheca Parva, Slg. Feige/Ale-Agha).

Incorporation of gypsum waste in ceramic block production: Proposal for a minimal battery of tests to evaluate technical and environmental viability of this recycling process.

Science.gov (United States)

Godinho-Castro, Alcione P; Testolin, Renan C; Janke, Leandro; Corrêa, Albertina X R; Radetski, Claudemir M

2012-01-01

Civil engineering-related construction and demolition debris is an important source of waste disposed of in municipal solid waste landfills. After clay materials, gypsum waste is the second largest contributor to the residential construction waste stream. As demand for sustainable building practices grows, interest in recovering gypsum waste from construction and demolition debris is increasing, but there is a lack of standardized tests to evaluate the technical and environmental viability of this solid waste recycling process. By recycling gypsum waste, natural deposits of gypsum might be conserved and high amounts of the waste by-product could be reused in the civil construction industry. In this context, this paper investigates a physical property (i.e., resistance to axial compression), the chemical composition and the ecotoxicological potential of ceramic blocks constructed with different proportions of clay, cement and gypsum waste, and assesses the feasibility of using a minimal battery of tests to evaluate the viability of this recycling process. Consideration of the results for the resistance to axial compression tests together with production costs revealed that the best formulation was 35% of plastic clay, 35% of non-plastic clay, 10% of Portland cement and 20% of gypsum waste, which showed a mean resistance of 4.64MPa. Energy dispersive X-ray spectrometry showed calcium and sulfur to be the main elements, while quartz, gypsum, ettringite and nacrite were the main crystalline compounds found in this formulation. Ecotoxicity tests showed that leachate from this formulation is weakly toxic toward daphnids and bacteria (EC(20%)=69.0 and 75.0, respectively), while for algae and fish the leachate samples were not toxic at the EC(50%) level. Overall, these results show that the addition of 20% of gypsum waste to the ceramic blocks could provide a viable substitute for clay in the ceramics industry and the tests applied in this study proved to be a useful tool

The effect of tube-support interaction on the dynamic response of heat exchanger tubes

International Nuclear Information System (INIS)

Shin, Y.S.; Jendrzejczyk, J.A.; Wambsganss, M.W.

1977-01-01

To avoid detrimental tube vibration in heat exchangers, resonant conditions and instabilitites must be avoided, and/or peak dynamic amplitudes must not exceed allowable limits. In attempting a theoretical analysis, questions arise as to the effects of tube/support interaction on tube vibrational characteristics (i.e. resonant frequencies, modes, damping) and response amplitude. As a part of ANL's Flow-Induced Vibration Program in support of the Clinch River Breeder Reactor Plant (CRBRP) steam generator design activity, tube/support interaction experiments are being performed not only to gain the insight into the dynamic behavior of CRBRP steam generator tubes, but also to provide the basis for developing design guidance. Test results were compared with anaytical results based on multispan tube with 'knife-edge' supports at the support locations. (Auth.)

Probabilistic Accident Progression Analysis with application to a LMFBR design

International Nuclear Information System (INIS)

Jamali, K.M.

1982-01-01

A method for probabilistic analysis of accident sequences in nuclear power plant systems referred to as ''Probabilistic Accident Progression Analysis'' (PAPA) is described. Distinctive features of PAPA include: (1) definition and analysis of initiator-dependent accident sequences on the component level; (2) a new fault-tree simplification technique; (3) a new technique for assessment of the effect of uncertainties in the failure probabilities in the probabilistic ranking of accident sequences; (4) techniques for quantification of dependent failures of similar components, including an iterative technique for high-population components. The methodology is applied to the Shutdown Heat Removal System (SHRS) of the Clinch River Breeder Reactor Plant during its short-term (0 -2 . Major contributors to this probability are the initiators loss of main feedwater system, loss of offsite power, and normal shutdown

Cost reduction through improved seismic design

International Nuclear Information System (INIS)

Severud, L.K.

1984-01-01

During the past decade, many significnt seismic technology developments have been accomplished by the United States Department of Energy (USDOE) programs. Both base technology and major projects, such as the Fast Flux Test Facility (FFTF) and the Clinch River Breeder Reactor (CRBR) plant, have contributed to seismic technology development and validation. Improvements have come in the areas of ground motion definitions, soil-structure interaction, and structural analysis methods and criteria for piping, equipment, components, reactor core, and vessels. Examples of some of these lessons learned and technology developments are provided. Then, the highest priority seismic technology needs, achievable through DOE actions and sponsorship are identified and discussed. Satisfaction of these needs are expected to make important contributions toward cost avoidances and reduced capital costs of future liquid metal nuclear plants. 23 references, 12 figures

Preliminary assessment of the radiological impact for individual waste management areas at the Oak Ridge National Laboratory: Status report

International Nuclear Information System (INIS)

Sears, M.B.

1987-09-01

This study estimates the radiological impact (i.e., the potential doses) for individual waste management areas at the Oak Ridge National Laboratory and ranks the areas for remedial action based on the off-site doses that result from these discharges to White Oak Creek. Dose estimates are given for the drinking water pathway based on known discharges from White Oak Dam. Estimates are also made of doses for eating fish caught in the Clinch River near the confluence with White Oak Creek. The results of a search for data concerning the discharges of 90 Sr, 3 H, 137 Cs, and 60 Co from individual waste management areas are presented. A qualitative assessment is presented, and areas are ranked for remedial investigation based on the available information. 29 refs., 8 figs., 45 tabs

Bronchoscopic resection of endobronchial inflammatory myofibroblastic tumor: A case report and systematic review of the literature

Directory of Open Access Journals (Sweden)

Animesh Ray

2014-01-01

Full Text Available Inflammatory myofibroblastic tumour (IMT is a rare tumour affecting the tracheo-bronchial tree in the adult population. The clinical presentation of this tumour is diverse and diagnosis can be definitively clinched by histopathological examination. Treatment of this tumour usually requires surgical resection with bronchoscopic resection being described in few cases. We describe a 32 year old male presenting with hemoptysis who was diagnosed to have IMT. Resection of the tumour was done with the help of rigid bronchoscopy. Post-resection, hemoptysis stopped and no recurrence of tumour was noted on subsequent follow-up. We also present a systematic review of literature of all the cases of tracheo-bronchial IMT treated with bronchoscopic resection and conclude it to be a useful alternative to surgery in such cases.