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Sample records for dominant-negative dmp53 extends

  1. Daxx-like protein of Drosophila interacts with Dmp53 and affects longevity and Ark mRNA level.

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    Bodai, László; Pardi, Norbert; Ujfaludi, Zsuzsanna; Bereczki, Orsolya; Komonyi, Orbán; Balint, Eva; Boros, Imre M

    2007-12-14

    Daxx-like protein (DLP), the Drosophila homolog of Daxx, binds Drosophila melanogaster p53 (Dmp53) through its C-terminal region. We generated DLP mutants and found that although DLP expression is developmentally regulated, it is not essential for the execution of the developmental program. The effects DLP mutations show in the loss of heterozygosity assay and on phenotypes resulting from Dmp53 overexpression indicate a genetic interaction between DLP and Dmp53. In contrast to Dmp53 mutants, however, loss of DLP does not result in radiosensitivity indicating that it does not play an essential role in the activation of Dmp53-dependent response after ionizing radiation, and DLP is also not required for the irradiation-induced activation of reaper. In contrast, DLP is involved in the transcriptional regulation of Ark, because Ark mRNA level is decreased in DLP mutants and increased upon ectopic overexpression of DLP. Interestingly, DLP mutants have reduced longevity and reduced female fertility. Altogether, our data suggest complex functions for DLP, which include an anti-apoptotic effect exerted through repression of some Dmp53 functions, and activation of some proapoptotic genes.

  2. Dmp53, basket and drICE gene knockdown and polyphenol gallic acid increase life span and locomotor activity in a Drosophila Parkinson's disease model

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    Hector Flavio Ortega-Arellano

    2013-01-01

    Full Text Available Understanding the mechanism(s by which dopaminergic (DAergic neurons are eroded in Parkinson's disease (PD is critical for effective therapeutic strategies. By using the binary tyrosine hydroxylase (TH-Gal4/UAS-X RNAi Drosophila melanogaster system, we report that Dmp53, basket and drICE gene knockdown in dopaminergic neurons prolong life span (p < 0.05; log-rank test and locomotor activity (p < 0.05; χ² test in D. melanogaster lines chronically exposed to (1 mM paraquat (PQ, oxidative stress (OS generator compared to untreated transgenic fly lines. Likewise, knockdown flies displayed higher climbing performance than control flies. Amazingly, gallic acid (GA significantly protected DAergic neurons, ameliorated life span, and climbing abilities in knockdown fly lines treated with PQ compared to flies treated with PQ only. Therefore, silencing specific gene(s involved in neuronal death might constitute an excellent tool to study the response of DAergic neurons to OS stimuli. We propose that a therapy with antioxidants and selectively "switching off" death genes in DAergic neurons could provide a means for pre-clinical PD individuals to significantly ameliorate their disease condition.

  3. Dmp53, basket and drICE gene knockdown and polyphenol gallic acid increase life span and locomotor activity in a Drosophila Parkinson’s disease model

    Science.gov (United States)

    Ortega-Arellano, Hector Flavio; Jimenez-Del-Rio, Marlene; Velez-Pardo, Carlos

    2013-01-01

    Understanding the mechanism(s) by which dopaminergic (DAergic) neurons are eroded in Parkinson’s disease (PD) is critical for effective therapeutic strategies. By using the binary tyrosine hydroxylase (TH)-Gal4/UAS-X RNAi Drosophila melanogaster system, we report that Dmp53, basket and drICE gene knockdown in dopaminergic neurons prolong life span (p < 0.05; log-rank test) and locomotor activity (p < 0.05; χ2 test) in D. melanogaster lines chronically exposed to (1 mM) paraquat (PQ, oxidative stress (OS) generator) compared to untreated transgenic fly lines. Likewise, knockdown flies displayed higher climbing performance than control flies. Amazingly, gallic acid (GA) significantly protected DAergic neurons, ameliorated life span, and climbing abilities in knockdown fly lines treated with PQ compared to flies treated with PQ only. Therefore, silencing specific gene(s) involved in neuronal death might constitute an excellent tool to study the response of DAergic neurons to OS stimuli. We propose that a therapy with antioxidants and selectively “switching off” death genes in DAergic neurons could provide a means for pre-clinical PD individuals to significantly ameliorate their disease condition. PMID:24385865

  4. The Ras mutant D119N is both dominant negative and activated

    NARCIS (Netherlands)

    Cool, RH; Schmidt, G; Lenzen, CU; Prinz, H; Vogt, D; Wittinghofer, A

    1999-01-01

    The introduction of mutation D119N (or its homolog) in the NKxD nucleotide binding motif of various Ras-like proteins produces constitutively activated or dominant-negative effects, depending on the system and assay. Here we show that Ras(D119N) has an inhibitory effect at a cell-specific concentrat

  5. Structure of the G60A mutant of Ras: Implications for the Dominant Negative Effect.

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    Ford,B.; Skowronek, K.; Boykevisch, S.; Bar-Sagi, D.; Nassar, N.

    2005-01-01

    Substituting alanine for glycine at position 60 in v-H-Ras generated a dominant negative mutant that completely abolished the ability of v-H-Ras to transform NIH 3T3 cells and to induce germinal vesicle breakdown in Xenopus oocytes. The crystal structure of the GppNp-bound form of RasG60A unexpectedly shows that the switch regions adopt an open conformation reminiscent of the structure of the nucleotide-free form of Ras in complex with Sos. Critical residues that normally stabilize the guanine nucleotide and the Mg{sup 2+} ion have moved considerably. Sos binds to RasG60A but is unable to catalyze nucleotide exchange. Our data suggest that the dominant negative effect observed for RasG60A{center_dot}GTP could result from the sequestering of Sos in a non-productive Ras-GTP-guanine nucleotide exchange factor ternary complex.

  6. PPARG in Human Adipogenesis: Differential Contribution of Canonical Transcripts and Dominant Negative Isoforms

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    M. Aprile

    2014-01-01

    Full Text Available The nuclear receptor PPARγ is a key regulator of adipogenesis, and alterations of its function are associated with different pathological processes related to metabolic syndrome. We recently identified two PPARG transcripts encoding dominant negative PPARγ isoforms. The existence of different PPARG variants suggests that alternative splicing is crucial to modulate PPARγ function, underlying some underestimated aspects of its regulation. Here we investigate PPARG expression in different tissues and cells affected in metabolic syndrome and, in particular, during adipocyte differentiation of human mesenchymal stem cells. We defined the transcript-specific expression pattern of PPARG variants encoding both canonical and dominant negative isoforms and identified a novel PPARG transcript, γ1ORF4. Our analysis indicated that, during adipogenesis, the transcription of alternative PPARG variants is regulated in a time-specific manner through differential usage of distinct promoters. In addition, our analysis describes—for the first time—the differential contribution of three ORF4 variants to this process, suggesting a still unexplored role for these dominant negative isoforms during adipogenesis. Therefore, our results highlight crucial aspects of PPARG regulation, suggesting the need of further investigation to rule out the differential impact of all PPARG transcripts in both physiologic and pathologic conditions, such as metabolism-related disorders.

  7. Cardioselective Dominant-negative Thyroid Hormone Receptor (Δ337T) Modulates Myocardial Metabolism and Contractile Dfficiency

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    Hyyti, Outi M.; Olson, Aaron; Ge, Ming; Ning, Xue-Han; Buroker, Norman E.; Chung, Youngran; Jue, Thomas; Portman, Michael A.

    2008-06-03

    Dominant- negative thyroid hormone receptors (TRs) show elevated expression relative to ligand-binding TRs during cardiac hypertrophy. We tested the hypothesis that overexpression of a dominant-negative TR alters cardiac metabolism and contractile efficiency (CE). We used mice expressing the cardioselective dominant-negative TRβ1 mutation Δ337T. Isolated working Δ337T hearts and nontransgenic control (Con) hearts were perfused with 13C-labeled free fatty acids (FFA), acetoacetate (ACAC), lactate, and glucose at physiological concentrations for 30 min. 13C NMR spectroscopy and isotopomer analyses were used to determine substrate flux and fractional contributions (Fc) of acetyl-CoA to the citric acid cycle (CAC). Δ337T hearts exhibited rate depression but higher developed pressure and CE, defined as work per oxygen consumption (MV˙ O2). Unlabeled substrate Fc from endogenous sources was higher in Δ337T, but ACAC Fc was lower. Fluxes through CAC, lactate, ACAC, and FFA were reduced in Δ337T. CE and Fc differences were reversed by pacing Δ337T to Con rates, accompanied by an increase in FFA Fc. Δ337T hearts lacked the ability to increase MV˙ O2. Decreases in protein expression for glucose transporter-4 and hexokinase-2 and increases in pyruvate dehydrogenase kinase-2 and -4 suggest that these hearts are unable to increase carbohydrate oxidation in response to stress. These data show that Δ337T alters the metabolic phenotype in murine heart by reducing substrate flux for multiple pathways. Some of these changes are heart rate dependent, indicating that the substrate shift may represent an accommodation to altered contractile protein kinetics, which can be disrupted by pacing stress.

  8. Inactivation of TNF Signaling by Rationally Designed Dominant-Negative TNF Variants

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    Steed, Paul M.; Tansey, Malú G.; Zalevsky, Jonathan; Zhukovsky, Eugene A.; Desjarlais, John R.; Szymkowski, David E.; Abbott, Christina; Carmichael, David; Chan, Cheryl; Cherry, Lisa; Cheung, Peter; Chirino, Arthur J.; Chung, Hyo H.; Doberstein, Stephen K.; Eivazi, Araz; Filikov, Anton V.; Gao, Sarah X.; Hubert, René S.; Hwang, Marian; Hyun, Linus; Kashi, Sandhya; Kim, Alice; Kim, Esther; Kung, James; Martinez, Sabrina P.; Muchhal, Umesh S.; Nguyen, Duc-Hanh T.; O'Brien, Christopher; O'Keefe, Donald; Singer, Karen; Vafa, Omid; Vielmetter, Jost; Yoder, Sean C.; Dahiyat, Bassil I.

    2003-09-01

    Tumor necrosis factor (TNF) is a key regulator of inflammatory responses and has been implicated in many pathological conditions. We used structure-based design to engineer variant TNF proteins that rapidly form heterotrimers with native TNF to give complexes that neither bind to nor stimulate signaling through TNF receptors. Thus, TNF is inactivated by sequestration. Dominant-negative TNFs represent a possible approach to anti-inflammatory biotherapeutics, and experiments in animal models show that the strategy can attenuate TNF-mediated pathology. Similar rational design could be used to engineer inhibitors of additional TNF superfamily cytokines as well as other multimeric ligands.

  9. Suppressing Autoimmunity in Arabidopsis thalianawith Dominant Negative Immune Receptors

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    Greeff, Michael Christiaan

    . CAMTA3 was previously shown to be a negative regulator of plant defense by inhibiting transcription of EDS1 and NDR1, important downstream signaling components of R-protein signaling. We found that two dominant negative alleles, DSC-D and DSC2-D, can suppress all tested camta3-1 phenotypes. We...... hypothesize that like acd11 and other autoimmune mutants, the increased levels of defense genes like EDS1 in camta3-1 might be a consequence of R protein activation and not merely as a result of negative regulation of plant defense responses as was previously proposed. DSC and CAMTA3 are part of a nuclear...

  10. Dominant-Negative Proteins in Herpesviruses – From Assigning Gene Function to Intracellular Immunization

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    Zsolt Ruzsics

    2009-10-01

    Full Text Available Investigating and assigning gene functions of herpesviruses is a process, which profits from consistent technical innovation. Cloning of bacterial artificial chromosomes encoding herpesvirus genomes permits nearly unlimited possibilities in the construction of genetically modified viruses. Targeted or randomized screening approaches allow rapid identification of essential viral proteins. Nevertheless, mapping of essential genes reveals only limited insight into function. The usage of dominant-negative (DN proteins has been the tool of choice to dissect functions of proteins during the viral life cycle. DN proteins also facilitate the analysis of host-virus interactions. Finally, DNs serve as starting-point for design of new antiviral strategies.

  11. A dominant negative zebrafish Ahr2 partially protects developing zebrafish from dioxin toxicity.

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    Kevin A Lanham

    Full Text Available The toxicity by 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD is thought to be caused by activation of the aryl hydrocarbon receptor (AHR. However, our understanding of how AHR activation by TCDD leads to toxic effects is poor. Ideally we would like to manipulate AHR activity in specific tissues and at specific times. One route to this is expressing dominant negative AHRs (dnAHRs. This work describes the construction and characterization of dominant negative forms of the zebrafish Ahr2 in which the C-terminal transactivation domain was either removed, or replaced with the inhibitory domain from the Drosophila engrailed repressor protein. One of these dnAhr2s was selected for expression from the ubiquitously active e2fα promoter in transgenic zebrafish. We found that these transgenic zebrafish expressing dnAhr2 had reduced TCDD induction of the Ahr2 target gene cyp1a, as measured by 7-ethoxyresorufin-O-deethylase activity. Furthermore, the cardiotoxicity produced by TCDD, pericardial edema, heart malformation, and reduced blood flow, were all mitigated in the zebrafish expressing the dnAhr2. These results provide in vivo proof-of-principle results demonstrating the effectiveness of dnAHRs in manipulating AHR activity in vivo, and demonstrating that this approach can be a means for blocking TCDD toxicity.

  12. Targeted disruption of Chlamydia trachomatis invasion by in trans expression of dominant negative Tarp effectors

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    Christopher J Parrett

    2016-08-01

    Full Text Available Chlamydia trachomatis invasion of eukaryotic host cells is facilitated, in part, by the type III secreted effector protein, Tarp. The role of Tarp in chlamydiae entry of host cells is supported by molecular approaches that examined recombinant Tarp or Tarp effectors expressed within heterologous systems. A major limitation in the ability to study the contribution of Tarp to chlamydial invasion of host cells was the prior absence of genetic tools for chlamydiae. Based on our knowledge of Tarp domain structure and function along with the introduction of genetic approaches in C. trachomatis, we hypothesized that Tarp function could be disrupted in vivo by the introduction of dominant negative mutant alleles. We provide evidence that transformed C. trachomatis produced epitope tagged Tarp, which was secreted into the host cell during invasion. We examined the effects of domain specific Tarp mutations on chlamydial invasion and growth and demonstrate that C. trachomatis clones harboring engineered Tarp mutants lacking either the actin binding domain or the phosphorylation domain had reduced levels of invasion into host cells. These data provide the first in vivo evidence for the critical role of Tarp in C. trachomatis pathogenesis and indicate that chlamydial invasion of host cells can be attenuated via the introduction of engineered dominant negative type three effectors.

  13. Suppression of Glioma-Cell Survival by Antisense and Dominant-Negative AKT2 RNA

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    Peiyu Pu; Chunsheng Kang; Jie Li; Guangxiu Wang

    2005-01-01

    OBJECTIVE Overexpression of growth factors and their receptors such as PDGF, FGF, VEGF, IGF, EGF, TGFα etc. Play a critical role in the development and progression of malignant gliomas. AKT, one of the most potent downstream signaling effectors of these growth factor receptors is usually overactivated in malignant gliomas. The present study was undertaken to investigate the effects of antisense and dominant negative AKT2 RNA on the survival of glioma cells with overexpression of AKT2.METHODS Antisense and dominant negative AKT2 constructs (AS-AKT2,DN-AKT2) were transfected into human glioblastoma cell line TJ905 with overexpression of AKT2. Using Western blotting, MTT assay, Ki67 labeling index (Ki67 LI), flow cytometry and the TUNEL method, the expression of AKT2 and GFAP, the proliferation rate and apoptosis of glioma cells transfected with AS-AKT2 or DN-AKT2 were compared to those characteristics of parental and glioma cells transfected with an empty vector.RESULTS Cell proliferation was inhibited in glioma cells transfected with ASAKT2 and DN-AKT2 RNA, while GFAP expression and apoptosis were markedly increased in those cells.CONCLUSION AKT is an important mediator in the growth signaling pathway of malignant gliomas and is a potential promising therapeutic target for malignant gliomas.

  14. Dental enamel structure is altered by expression of dominant negative RhoA in ameloblasts.

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    Li, Yong; Pugach, Megan K; Kuehl, Melissa A; Peng, Li; Bouchard, Jessica; Hwang, Soon Y; Gibson, Carolyn W

    2011-01-01

    Using in vitrotooth germ cultures and analysis by confocal microscopy, ameloblasts treated with sodium fluoride were found to have elevated amounts of filamentous actin. Because this response is reduced by inhibitors of the Rho/ROCK signaling pathway, we generated mice that express dominant negative RhoA (RhoA(DN)) in ameloblasts for in vivo analysis. Expression of the EGFP-RhoA(DN) fusion protein was evaluated by RT-PCR and immunohistochemistry, and teeth were analyzed by scanning electron microscopy. The 3 strains expressed at either low (TgEGFP-RhoA(DN)-8), intermediate (TgEGFP-RhoA(DN)-2), or high (TgEGFP-RhoA(DN)-13) levels, and the molar teeth from the 3 strains had enamel hypoplasia and surface defects. We conclude that RhoA(DN) expressed in ameloblasts interferes with normal enamel development through the pathway that is induced by sodium fluoride. Copyright © 2011 S. Karger AG, Basel.

  15. Suppressing autoimmunity in Arabidopsis thaliana with dominant negative immune receptors

    DEFF Research Database (Denmark)

    Greeff, Michael Christiaan

    2014-01-01

    targets. In response to effector mediated changes on targets, R proteins trigger the hypersensitive response. We have proposed that a corollary to this 'guard model' is that forms of plant autoimmunity are due to inappropriate R protein activation. For example, we showed that a knockout of Accelerated...... Cell Death 11 (acd11) leads to inappropriate activation of hypersensitive cell death. We have previously performed a large-scale survival screen for suppressors of acd11 and found that cell death in acd11 is suppressed by mutations in a gene encoding an R protein. We have thus proposed that loss of ACD......11 results in HR cell death because LAZ5 directly or indirectly guards it. The LAZ5 alleles we first found were dominant negative (laz5-DN). The laz-DN allele mutation was found in a conserved functionally important ATP binding region, the P-loop. Site-directed DN mutant alleles can be made for other...

  16. Reduced striatal dopamine DA D2 receptor function in dominant-negative GSK-3 transgenic mice.

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    Gomez-Sintes, Raquel; Bortolozzi, Analia; Artigas, Francesc; Lucas, José J

    2014-09-01

    Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase with constitutive activity involved in cellular architecture, gene expression, cell proliferation, fate decision and apoptosis, among others. GSK-3 expression is particularly high in brain where it may be involved in neurological and psychiatric disorders such as Alzheimer׳s disease, bipolar disorder and major depression. A link with schizophrenia is suggested by the antipsychotic drug-induced GSK-3 regulation and by the involvement of the Akt/GSK-3 pathway in dopaminergic neurotransmission. Taking advantage of the previous development of dominant negative GSK-3 transgenic mice (Tg) showing a selective reduction of GSK-3 activity in forebrain neurons but not in dopaminergic neurons, we explored the relationship between GSK-3 and dopaminergic neurotransmission in vivo. In microdialysis experiments, local quinpirole (DA D2-R agonist) in dorsal striatum reduced dopamine (DA) release significantly less in Tg mice than in wild-type (WT) mice. However, local SKF-81297 (selective DA D1-R agonist) in dorsal striatum reduced DA release equally in both control and Tg mice indicating a comparable function of DA D1-R in the direct striato-nigral pathway. Likewise, systemic quinpirole administration - acting preferentially on presynaptic DA D2- autoreceptors to modulate DA release-reduced striatal DA release similarly in both control and Tg mice. Quinpirole reduced locomotor activity and induced c-fos expression in globus pallidus (both striatal DA D2-R-mediated effects) significantly more in WT than in Tg mice. Taking together, the present results show that dominant negative GSK-3 transgenic mice show reduced DA D2-R-mediated function in striatum and further support a link between dopaminergic neurotransmission and GSK-3 activity.

  17. Mutations of nuclear localization signals in mNANOG generate dominant negative mutants

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    ZHANG Juan; ZHANG XiaoFei; PEI DuanQing

    2009-01-01

    Mouse NANOG plays a critical role in maintaining self-renewal and pluripotency of embryonic stem cells.Yet,the precise mechanism of how mNANOG functions is still less known.Here,we report that mouse NANOG has two nuclear localization signals (NLS,RKQKMR and RMKCKR) which are respon-sible for the nuclear localization and transcriptional activity in the conserved homeobox domain.NLS mutants of mouse NANOG generate:3 mutants that are localized throughout the cells and lose the transectivation function.We further prove that all three NLS mutants may interact with the wild-type mouse NANOG like NANOG dimerization itself and inhibit the wild-type mouse NANOG activity,acting as dominant negative mutants.The NLS mutants of mouse NANOG may also inhibit activity of oct4 promoter in pluripotent cells,indicating that the NLS mutants can affect the endogenous mouse NANOG function in vivo.These data suggest that the NLS mutants of mouse NANOG may be used as a tool to regulate NANOG activity in pluripotent cells.

  18. Study of Vaccinia and Cowpox viruses' replication in Rac1-N17 dominant-negative cells

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    Ana Paula Carneiro Salgado

    2013-08-01

    Full Text Available Interfering with cellular signal transduction pathways is a common strategy used by many viruses to create a propitious intracellular environment for an efficient replication. Our group has been studying cellular signalling pathways activated by the orthopoxviruses Vaccinia (VACV and Cowpox (CPXV and their significance to viral replication. In the present study our aim was to investigate whether the GTPase Rac1 was an upstream signal that led to the activation of MEK/ERK1/2, JNK1/2 or Akt pathways upon VACV or CPXV' infections. Therefore, we generated stable murine fibroblasts exhibiting negative dominance to Rac1-N17 to evaluate viral growth and the phosphorylation status of ERK1/2, JNK1/2 and Akt. Our results demonstrated that VACV replication, but not CPXV, was affected in dominant-negative (DN Rac1-N17 cell lines in which viral yield was reduced in about 10-fold. Viral late gene expression, but not early, was also reduced. Furthermore, our data showed that Akt phosphorylation was diminished upon VACV infection in DN Rac1-N17 cells, suggesting that Rac1 participates in the phosphoinositide-3 kinase pathway leading to the activation of Akt. In conclusion, our results indicate that while Rac1 indeed plays a role in VACV biology, perhaps another GTPase may be involved in CPXV replication.

  19. VP22 fusion protein-based dominant negative mutant can inhibit hepatitis B virus replication

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    Jun Yi; Wei-Dong Gong; Ling Wang; Rui Ling; Jiang-Hao Chen; Jun Yun

    2005-01-01

    AIM: To investigate the inhibitory effect of VP22 fusion protein-based dominant negative (DN) mutant on Hepatitis Bvrus (HBV) replication.METHODS: Full-length or truncated fragment of VP22 was fused to C terminal of HBV core protein (HBc), and subcloned into pcDNA3.1 (-) vector, yielding eukaryotic expression plasmids of DN mutant. After transfection into HepG2.2.15 cells, the expression of DN mutant was identified by immunofluorescence staining. The inhibitory effect of DN mutant on HBV replication was indexed as the supernatant HBsAg concentration determined by RIA and HBV-DNA content by fluorescent quantification-PCR (FQ-PCR). Meanwhile, metabolism of HepG2.2.15 cells was evaluated by MTT colorimetry.RESULTS: VP22-based DN mutants and its truncated fragment were expressed in HepG2.2.15 cells, and had no toxic effect on host cells. DN mutants could inhibit HBV replication and the transduction ability of mutantbearing protein had a stronger inhibitory effect on HBV replication. DN mutants with full length of VP22 had the strongest inhibitory effect on HBV replication, reducing the HBsAg concentration by 81.94%, and the HBV-DNA content by 72.30%. MTT assay suggested that there were no significant differences in cell metabolic activity between the groups.CONCLUSION: VP22-based DN mutant can inhibit HBV replication effectively.

  20. A recurrent dominant negative E47 mutation causes agammaglobulinemia and BCR(-) B cells.

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    Boisson, Bertrand; Wang, Yong-Dong; Bosompem, Amma; Ma, Cindy S; Lim, Annick; Kochetkov, Tatiana; Tangye, Stuart G; Casanova, Jean-Laurent; Conley, Mary Ellen

    2013-11-01

    Approximately 90% of patients with isolated agammaglobulinemia and failure of B cell development have mutations in genes required for signaling through the pre–B cell and B cell receptors. The nature of the gene defect in the majority of remaining patients is unknown. We recently identified 4 patients with agammaglobulinemia and markedly decreased numbers of peripheral B cells. The B cells that could be detected had an unusual phenotype characterized by the increased expression of CD19 but the absence of a B cell receptor. Genetic studies demonstrated that all 4 patients had the exact same de novo mutation in the broadly expressed transcription factor E47. The mutant protein (E555K) was stable in patient-derived EBV-transformed cell lines and cell lines transfected with expression vectors. E555K in the transfected cells localized normally to the nucleus and resulted in a dominant negative effect when bound to DNA as a homodimer with wild-type E47. Mutant E47 did permit DNA binding by a tissue-specific heterodimeric DNA-binding partner, myogenic differentiation 1 (MYOD). These findings document a mutational hot-spot in E47 and represent an autosomal dominant form of agammaglobulinemia. Further, they indicate that E47 plays a critical role in enforcing the block in development of B cell precursors that lack functional antigen receptors.

  1. Generic and personalized RNAi-based therapeutics for a dominant-negative epidermal fragility disorder.

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    Leslie Pedrioli, Deena M; Fu, Dun Jack; Gonzalez-Gonzalez, Emilio; Contag, Christopher H; Kaspar, Roger L; Smith, Frances J D; McLean, W H Irwin

    2012-06-01

    Epidermolytic palmoplantar keratoderma (EPPK) is one of >30 autosomal-dominant human keratinizing disorders that could benefit from RNA interference (RNAi)-based therapy. EPPK is caused by mutations in the keratin 9 (KRT9) gene, which is exclusively expressed in thick palm and sole skin where there is considerable keratin redundancy. This, along with the fact that EPPK is predominantly caused by a few hotspot mutations, makes it an ideal proof-of-principle model skin disease to develop gene-specific, as well as mutation-specific, short interfering RNA (siRNA) therapies. We have developed a broad preclinical RNAi-based therapeutic package for EPPK containing generic KRT9 siRNAs and allele-specific siRNAs for four prevalent mutations. Inhibitors were systematically identified in vitro using a luciferase reporter gene assay and validated using an innovative dual-Flag/Strep-TagII quantitative immunoblot assay. siKRT9-1 and siKRT9-3 were the most potent generic K9 inhibitors, eliciting >85% simultaneous knockdown of wild-type and mutant K9 protein synthesis at picomolar concentrations. The allele-specific inhibitors displayed similar potencies and, importantly, exhibited strong specificities for their target dominant-negative alleles with little or no effect on wild-type K9. The most promising allele-specific siRNA, siR163Q-13, was tested in a mouse model and was confirmed to preferentially inhibit mutant allele expression in vivo.

  2. A cancer-predisposing "hot spot" mutation of the fumarase gene creates a dominant negative protein.

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    Lorenzato, Annalisa; Olivero, Martina; Perro, Mario; Brière, Jean Jacques; Rustin, Pierre; Di Renzo, Maria Flavia

    2008-02-15

    The Fumarase (Fumarate Hydratase, FH) is a tumor suppressor gene whose germline heterozygous mutations predispose to hereditary leiomyomatosis and renal cell cancer (HLRCC). The FH gene encodes an enzyme of the Krebs cycle, functioning as a homotetramer and catalyzing the hydration of fumarate to malate. Among the numerous FH mutations reported so far, the R190H missense mutation is the most frequent in HLRCC patients. Here we show the functional analyses of the R190H, in comparison to the better characterized E319Q mutation. We first expressed wild-type and mutated proteins in FH deficient human skin fibroblasts, using lentiviral vectors. The wild-type transgene was able to restore the FH enzymatic activity in cells, while the R190H- and E319Q-FH were not. More interestingly, when the same transgenes were expressed in normal, FH-proficient cells, only the R190H-FH reduced the endogenous FH enzymatic activity. By enforcing the expression of equal amount of wild-type and R190H-FH in the same cell, we showed that the mutated FH protein directly inhibited enzymatic activity by nearly abrogating the FH homotetramer formation. These data demonstrate the dominant negative effect of the R190H missense mutation in the FH gene and suggest that the FH tumor-suppressing activity might be impaired in cells carrying a heterozygous mutation. (c) 2007 Wiley-Liss, Inc.

  3. Expression of a dominant negative PKA mutation in the kidney elicits a diabetes insipidus phenotype.

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    Gilbert, Merle L; Yang, Linghai; Su, Thomas; McKnight, G Stanley

    2015-03-15

    PKA plays a critical role in water excretion through regulation of the production and action of the antidiuretic hormone arginine vasopressin (AVP). The AVP prohormone is produced in the hypothalamus, where its transcription is regulated by cAMP. Once released into the circulation, AVP stimulates antidiuresis through activation of vasopressin 2 receptors in renal principal cells. Vasopressin 2 receptor activation increases cAMP and activates PKA, which, in turn, phosphorylates aquaporin (AQP)2, triggering apical membrane accumulation, increased collecting duct permeability, and water reabsorption. We used single-minded homolog 1 (Sim1)-Cre recombinase-mediated expression of a dominant negative PKA regulatory subunit (RIαB) to disrupt kinase activity in vivo and assess the role of PKA in fluid homeostasis. RIαB expression gave rise to marked polydipsia and polyuria; however, neither hypothalamic Avp mRNA expression nor urinary AVP levels were attenuated, indicating a primary physiological effect on the kidney. RIαB mice displayed a marked deficit in urinary concentrating ability and greatly reduced levels of AQP2 and phospho-AQP2. Dehydration induced Aqp2 mRNA in the kidney of both control and RIαB-expressing mice, but AQP2 protein levels were still reduced in RIαB-expressing mutants, and mice were unable to fully concentrate their urine and conserve water. We conclude that partial PKA inhibition in the kidney leads to posttranslational effects that reduce AQP2 protein levels and interfere with apical membrane localization. These findings demonstrate a distinct physiological role for PKA signaling in both short- and long-term regulation of AQP2 and characterize a novel mouse model of diabetes insipidus.

  4. [Functional analysis of transforming growth factor-beta type II dominant negative receptor].

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    Takarada, M

    1996-06-01

    The transforming growth factor-beta (TGF-beta) is a multifunctional homodimeric protein with an apparent molecular weight of 25 KDa. TGF-beta transduces signals by forming heteromeric complexes of their type-I (T beta R-I) and type-II (T beta R-II) serin/threonine kinase receptors. TGF-beta binds first to T beta R-II receptor, and then the ligand in this complex is recognized by T beta R-I, resulting in formation of a heteromeric receptor complex composed of T beta R-I and T beta R-II. Once received, T beta R-I becomes phosphorylated in the GS domain by the associated constitutively active T beta R-II and transmits the downstream signal. It has been reported that formation of the heteromeric complex is indispensible at least in epithelial cells for growth inhibition and extracellular matrix production induced by TGF-beta. In this study, the functional role of T beta R-II for the TGF-beta-induced signals in osteoblastic cells was investigated by using a dominant negative type of T beta R-II mutant receptors (T beta RIIDNR). ROS 17/2.8 and MG 63 cells were found to express T beta R-I, T beta R-II, and T beta R-III, and their cell growth was inhibited by TGF-beta, whereas alkaline phosphatase activity was stimulated. Cells that were stably transfected with the T beta RIIDNR plasmid showed decreased response to TGF-beta during growth and alkaline phosphatase activity. These results indicate that the intracellular serine/threonine kinase domain of T beta R-II is essential for signal transduction of the TGF-beta-induced alkaline phosphatase activity as well as growth inhibition.

  5. Generation of a Retinoblastoma (Rb)1-inducible dominant-negative (DN) mouse model.

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    Tarang, Shikha; Doi, Songila M S R; Gurumurthy, Channabasavaiah B; Harms, Donald; Quadros, Rolen; Rocha-Sanchez, Sonia M

    2015-01-01

    Retinoblastoma 1 (Rb1) is an essential gene regulating cellular proliferation, differentiation, and homeostasis. To exert these functions, Rb1 is recruited and physically interacts with a growing variety of signaling pathways. While Rb1 does not appear to be ubiquitously expressed, its expression has been confirmed in a variety of hematopoietic and neuronal-derived cells, including the inner ear hair cells (HCs). Studies in transgenic mice demonstrate that complete germline or conditional Rb1 deletion leads to abnormal cell proliferation, followed by massive apoptosis; making it difficult to fully address Rb1's biochemical activities. To overcome these limitations, we developed a tetracycline-inducible TetO-CB-myc6-Rb1 (CBRb) mouse model to achieve transient and inducible dominant-negative (DN) inhibition of the endogenous RB1 protein. Our strategy involved fusing the Rb1 gene to the lysosomal protease pre-procathepsin B (CB), thus allowing for further routing of the DN-CBRb fusion protein and its interacting complexes for proteolytic degradation. Moreover, reversibility of the system is achieved upon suppression of doxycycline (Dox) administration. Preliminary characterization of DN-CBRb mice bred to a ubiquitous rtTA mouse line demonstrated a significant inhibition of the endogenous RB1 protein in the inner ear and in a number of other organs where RB1 is expressed. Examination of the postnatal (P) DN-CBRb mice inner ear at P10 and P28 showed the presence of supernumerary inner HCs (IHCs) in the lower turns of the cochleae, which corresponds to the described expression domain of the endogenous Rb1 gene. Selective and reversible suppression of gene expression is both an experimental tool for defining function and a potential means to medical therapy. Given the limitations associated with Rb1-null mice lethality, this model provides a valuable resource for understanding RB1 activity, relative contribution to HC regeneration and its potential therapeutic application.

  6. Dominant negative Ras attenuates pathological ventricular remodeling in pressure overload cardiac hypertrophy

    Science.gov (United States)

    Ramos-Kuri, Manuel; Rapti, Kleopatra; Mehel, Hind; Zhang, Shihong; Dhandapany, Perundurai S.; Liang, Lifan; García-Carrancá, Alejandro; Bobe, Regis; Fischmeister, Rodolphe; Adnot, Serge; Lebeche, Djamel; Hajjar, Roger J.; Lipskaia, Larissa; Chemaly, Elie R.

    2015-01-01

    The importance of the oncogene Ras in cardiac hypertrophy is well appreciated. The hypertrophic effects of the constitutively active mutant Ras-Val12 are revealed by clinical syndromes due to the Ras mutations and experimental studies. We examined the possible anti-hypertrophic effect of Ras inhibition in vitro using rat neonatal cardiomyocytes (NRCM) and in vivo in the setting of pressure-overload left ventricular (LV) hypertrophy (POH) in rats. Ras functions were modulated via adenovirus directed gene transfer of active mutant Ras-Val12 or dominant negative mutant N17-DN-Ras (DN-Ras). Ras-Val12 expression in vitro activates NFAT resulting in pro-hypertrophic and cardio-toxic effects on NRCM beating and Z-line organization. In contrast, the DN-Ras was antihypertrophic on NRCM, inhibited NFAT and exerted cardio-protective effects attested by preserved NRCM beating and Z line structure. Additional experiments with silencing H-Ras gene strategy corroborated the antihypertrophic effects of siRNA-H-Ras on NRCM. In vivo, with the POH model, both Ras mutants were associated with similar hypertrophy two weeks after simultaneous induction of POH and Ras-mutant gene transfer. However, LV diameters were higher and LV fractional shortening lower in the Ras-Val12 group compared to control and DN-Ras. Moreover, DN-Ras reduced the cross-sectional area of cardiomyocytes in vivo, and decreased the expression of markers of pathologic cardiac hypertrophy. In isolated adult cardiomyocytes after 2 weeks of POH and Ras-mutant gene transfer, DN-Ras improved sarcomere shortening and calcium transients compared to Ras-Val12. Overall, DN-Ras promotes a more physiological form of hypertrophy, suggesting an interesting therapeutic target for pathological cardiac hypertrophy. PMID:26260012

  7. Regulation of hippocampal synaptic plasticity thresholds and changes in exploratory and learning behavior in dominant negative NPR-B mutant rats

    Science.gov (United States)

    Barmashenko, Gleb; Buttgereit, Jens; Herring, Neil; Bader, Michael; Özcelik, Cemil; Manahan-Vaughan, Denise; Braunewell, Karl H.

    2014-01-01

    The second messenger cyclic GMP affects synaptic transmission and modulates synaptic plasticity and certain types of learning and memory processes. The impact of the natriuretic peptide receptor B (NPR-B) and its ligand C-type natriuretic peptide (CNP), one of several cGMP producing signaling systems, on hippocampal synaptic plasticity and learning is, however, less well understood. We have previously shown that the NPR-B ligand CNP increases the magnitude of long-term depression (LTD) in hippocampal area CA1, while reducing the induction of long-term potentiation (LTP). We have extended this line of research to show that bidirectional plasticity is affected in the opposite way in rats expressing a dominant-negative mutant of NPR-B (NSE-NPR-BΔKC) lacking the intracellular guanylyl cyclase domain under control of a promoter for neuron-specific enolase. The brain cells of these transgenic rats express functional dimers of the NPR-B receptor containing the dominant-negative NPR-BΔKC mutant, and therefore show decreased CNP-stimulated cGMP-production in brain membranes. The NPR-B transgenic rats display enhanced LTP but reduced LTD in hippocampal slices. When the frequency-dependence of synaptic modification to afferent stimulation in the range of 1–100 Hz was assessed in transgenic rats, the threshold for both, LTP and LTD induction, was shifted to lower frequencies. In parallel, NPR-BΔKC rats exhibited an enhancement in exploratory and learning behavior. These results indicate that bidirectional plasticity and learning and memory mechanism are affected in transgenic rats expressing a dominant-negative mutant of NPR-B. Our data substantiate the hypothesis that NPR-B-dependent cGMP signaling has a modulatory role for synaptic information storage and learning. PMID:25520616

  8. Regulation of hippocampal synaptic plasticity thresholds and changes in exploratory and learning behavior in dominant negative NPR-B mutant rats

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    Gleb eBarmashenko

    2014-12-01

    Full Text Available The second messenger cyclic GMP affects synaptic transmission and modulates synaptic plasticity and certain types of learning and memory processes. The impact of the natriuretic peptide receptor B (NPR-B and its ligand C-type natriuretic peptide (CNP, one of several cGMP producing signalling systems, on hippocampal synaptic plasticity and learning is, however, less well understood. We have previously shown that the NPR-B ligand CNP increases the magnitude of long-term depression (LTD in hippocampal area CA1, while reducing the induction of long-term potentiation (LTP. We have extended this line of research to show that bidirectional plasticity is affected in the opposite way in rats expressing a dominant-negative mutant of NPR-B (NSE-NPR-BdeltaKC lacking the intracellular guanylyl cyclase domain under control of a promoter for neuron-specific enolase. The brain cells of these transgenic rats express functional dimers of the NPR-B receptor containing the dominant-negative NPR-BdeltaKC mutant, and therefore show decreased CNP-stimulated cGMP-production in brain membranes. The NPR-B transgenic rats display enhanced LTP but reduced LTD in hippocampal slices. When the frequency-dependence of synaptic modification to afferent stimulation in the range of 1-100 Hz was assessed in transgenic rats the threshold for LTP induction was raised, but LTD induction was facilitated. In parallel, NPR-BdeltaKC rats exhibited an enhancement in exploratory and learning behavior. These results indicate that bidirectional plasticity and learning and memory mechanism are affected in transgenic rats expressing a dominant-negative mutant of NPR-B. Our data substantiate the hypothesis that NPR-B-dependent cGMP signalling has a modulatory role for synaptic information storage and learning.

  9. Dominant Negative Mutants of the Estrogen Receptor as Probes of Estrogen Action and Inhibitors of Breast Cancer Growth

    Science.gov (United States)

    1996-07-01

    Ishikawa and BG-1 cells ( Ignar -Trowbridge et al., 1993). Based upon this result, we tested whether dominant negative ER mutants could inhibit growth...early promoter, which is the basal promoter of one of the ERE reporter plasmids used by Ignar -Trowbridge et al. We also expanded our experiments to...14032 Danielan PS, White R, Lees HA & Parker MG, 1992, EMBO J 11:1025-1033 Ignar -Trowbridge DM, Teng CT, Ross KA, Parker MG, Korach KS & McLachlan JA 1993

  10. Characterization of two second-site mutations preventing wild type protein aggregation caused by a dominant negative PMA1 mutant.

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    Pilar Eraso

    Full Text Available The correct biogenesis and localization of Pma1 at the plasma membrane is essential for yeast growth. A subset of PMA1 mutations behave as dominant negative because they produce aberrantly folded proteins that form protein aggregates, which in turn provoke the aggregation of the wild type protein. One approach to understand this dominant negative effect is to identify second-site mutations able to suppress the dominant lethal phenotype caused by those mutant alleles. We isolated and characterized two intragenic second-site suppressors of the PMA1-D378T dominant negative mutation. We present here the analysis of these new mutations that are located along the amino-terminal half of the protein and include a missense mutation, L151F, and an in-frame 12bp deletion that eliminates four residues from Cys409 to Ala412. The results show that the suppressor mutations disrupt the interaction between the mutant and wild type enzymes, and this enables the wild type Pma1 to reach the plasma membrane.

  11. Rapid degradation of dominant-negative Rab27 proteins in vivo precludes their use in transgenic mouse models

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    Huxley Clare

    2002-10-01

    Full Text Available Abstract Background Transgenic mice have proven to be a powerful system to study normal and pathological gene functions. Here we describe an attempt to generate a transgenic mouse model for choroideremia (CHM, a slow-onset X-linked retinal degeneration caused by mutations in the Rab Escort Protein-1 (REP1 gene. REP1 is part of the Rab geranylgeranylation machinery, a modification that is essential for Rab function in membrane traffic. The loss of REP1 in CHM patients may trigger retinal degeneration through its effects on Rab proteins. We have previously reported that Rab27a is the Rab most affected in CHM lymphoblasts and hypothesised that the selective dysfunction of Rab27a (and possibly a few other Rab GTPases plays an essential role in the retinal degenerative process. Results To investigate this hypothesis, we generated several lines of dominant-negative, constitutively-active and wild-type Rab27a (and Rab27b transgenic mice whose expression was driven either by the pigment cell-specific tyrosinase promoter or the ubiquitous β-actin promoter. High levels of mRNA and protein were observed in transgenic lines expressing wild-type or constitutively active Rab27a and Rab27b. However, only modest levels of transgenic protein were expressed. Pulse-chase experiments suggest that the dominant-negative proteins, but not the constitutively-active or wild type proteins, are rapidly degraded. Consistently, no significant phenotype was observed in our transgenic lines. Coat-colour was normal, indicating normal Rab27a activity. Retinal function as determined by fundoscopy, angiography, electroretinography and histology was also normal. Conclusions We suggest that the instability of the dominant-negative mutant Rab27 proteins in vivo precludes the use of this approach to generate mouse models of disease caused by Rab27 GTPases.

  12. An ABCA1 truncation shows no dominant negative effect in a familial hypoalphalipoproteinemia pedigree with three ABCA1 mutations

    Energy Technology Data Exchange (ETDEWEB)

    Sorrenson, Brie; Suetani, Rachel J. [Department of Biochemistry, University of Otago, Dunedin (New Zealand); Bickley, Vivienne M.; George, Peter M. [Clinical Biochemistry, Canterbury Health Laboratories, Christchurch (New Zealand); Williams, Michael J.A. [Department of Medicine, University of Otago, Dunedin (New Zealand); Scott, Russell S. [Lipid and Diabetes Research Group, Christchurch Hospital (New Zealand); McCormick, Sally P.A., E-mail: sally.mccormick@otago.ac.nz [Department of Biochemistry, University of Otago, Dunedin (New Zealand)

    2011-06-10

    Highlights: {yields} Characterisation of an ABCA1 truncation mutant, C978fsX988, in a pedigree with three ABCA1 mutations. {yields} Functional analysis of C978fsX988 in patient fibroblasts and HEK 293 cells shows no cholesterol efflux function. {yields} Allele-specific quantification shows C978fsX988 not expressed at mRNA level in fibroblasts. {yields} Unlike other ABCA1 truncations, C978fsX988 mutant shows no dominant negative effect at mRNA or protein level. -- Abstract: The ATP binding cassette transporter (ABCA1) A1 is a key determinant of circulating high density lipoprotein cholesterol (HDL-C) levels. Mutations in ABCA1 are a major genetic contributor to low HDL-C levels within the general population. Following the finding of three different ABCA1 mutations, p.C978fsX988, p.T1512M and p.N1800H in a subject with hypoalphalipoproteinemia, we aimed to establish whether the p.C978fsX988 truncation exerted a dominant negative effect on the full-length ABCA1 alleles within family members as has been reported for other ABCA1 truncations. Characterisation of the p.C978fsX988 mutant in transfected HEK 293 cells showed it to be expressed as a GFP fusion protein but lacking in cholesterol efflux function. This was in keeping with results from cholesterol efflux assays in the fibroblasts of p.C978fsX988 carriers which also showed impaired efflux. Allele- specific quantification of p.C978fsX988 mRNA and analysis of ABCA1 protein levels in the fibroblasts of p.C978fsX988 heterozygotes showed negligible levels of mRNA and protein expression. There was no evidence of a dominant negative effect on wildtype or p.N1800H protein levels. We conclude that in the case of the p.C978fsX988 truncated mutant a lack of expression precludes it from having a dominant negative effect.

  13. Characterization of a dominant negative C. elegans Twist mutant protein with implications for human Saethre-Chotzen syndrome.

    Science.gov (United States)

    Corsi, Ann K; Brodigan, Thomas M; Jorgensen, Erik M; Krause, Michael

    2002-06-01

    Twist is a transcription factor that is required for mesodermal cell fates in all animals studied to date. Mutations of this locus in humans have been identified as the cause of the craniofacial disorder Saethre-Chotzen syndrome. The Caenorhabditis elegans Twist homolog is required for the development of a subset of the mesoderm. A semidominant allele of the gene that codes for CeTwist, hlh-8, has defects that occur earlier in the mesodermal lineage than a previously studied null allele of the gene. The semidominant allele has a charge change (E29K) in the basic DNA-binding domain of CeTwist. Surprisingly, the mutant protein retains DNA-binding activity as both a homodimer and a heterodimer with its partner E/Daughterless (CeE/DA). However, the mutant protein blocks the activation of the promoter of a target gene. Therefore, the mutant CeTwist may cause cellular defects as a dominant negative protein by binding to target promoters as a homo- or heterodimer and then blocking transcription. Similar phenotypes as those caused by the E29K mutation were observed when amino acid substitutions in the DNA-binding domain that are associated with the human Saethre-Chotzen syndrome were engineered into the C. elegans protein. These data suggest that Saethre-Chotzen syndrome may be caused, in some cases, by dominant negative proteins, rather than by haploinsufficiency of the locus.

  14. Non dominant-negative KCNJ2 gene mutations leading to Andersen-Tawil syndrome with an isolated cardiac phenotype.

    Science.gov (United States)

    Limberg, Maren M; Zumhagen, Sven; Netter, Michael F; Coffey, Alison J; Grace, Andrew; Rogers, Jane; Böckelmann, Doris; Rinné, Susanne; Stallmeyer, Birgit; Decher, Niels; Schulze-Bahr, Eric

    2013-05-01

    Andersen-Tawil syndrome (ATS) is characterized by dysmorphic features, periodic paralyses and abnormal ventricular repolarization. After genotyping a large set of patients with congenital long-QT syndrome, we identified two novel, heterozygous KCNJ2 mutations (p.N318S, p.W322C) located in the C-terminus of the Kir2.1 subunit. These mutations have a different localization than classical ATS mutations which are mostly located at a potential interaction face with the slide helix or at the interface between the C-termini. Mutation carriers were without the key features of ATS, causing an isolated cardiac phenotype. While the N318S mutants regularly reached the plasma membrane, W322C mutants primarily resided in late endosomes. Co-expression of N318S or W322C with wild-type Kir2.1 reduced current amplitudes only by 20-25 %. This mild loss-of-function for the heteromeric channels resulted from defective channel trafficking (W322C) or gating (N318S). Strikingly, and in contrast to the majority of ATS mutations, neither mutant caused a dominant-negative suppression of wild-type Kir2.1, Kir2.2 and Kir2.3 currents. Thus, a mild reduction of native Kir2.x currents by non dominant-negative mutants may cause ATS with an isolated cardiac phenotype.

  15. Rhodopsin gene expression determines rod outer segment size and rod cell resistance to a dominant-negative neurodegeneration mutant.

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    Brandee A Price

    Full Text Available Two outstanding unknowns in the biology of photoreceptors are the molecular determinants of cell size, which is remarkably uniform among mammalian species, and the mechanisms of rod cell death associated with inherited neurodegenerative blinding diseases such as retinitis pigmentosa. We have addressed both questions by performing an in vivo titration with rhodopsin gene copies in genetically engineered mice that express only normal rhodopsin or an autosomal dominant allele, encoding rhodopsin with a disease-causing P23H substitution. The results reveal that the volume of the rod outer segment is proportional to rhodopsin gene expression; that P23H-rhodopsin, the most common rhodopsin gene disease allele, causes cell death via a dominant-negative mechanism; and that long term survival of rod cells carrying P23H-rhodopsin can be achieved by increasing the levels of wild type rhodopsin. These results point to promising directions in gene therapy for autosomal dominant neurodegenerative diseases caused by dominant-negative mutations.

  16. The mitochondrial calcium uniporter is a multimer that can include a dominant-negative pore-forming subunit.

    Science.gov (United States)

    Raffaello, Anna; De Stefani, Diego; Sabbadin, Davide; Teardo, Enrico; Merli, Giulia; Picard, Anne; Checchetto, Vanessa; Moro, Stefano; Szabò, Ildikò; Rizzuto, Rosario

    2013-08-28

    Mitochondrial calcium uniporter (MCU) channel is responsible for Ruthenium Red-sensitive mitochondrial calcium uptake. Here, we demonstrate MCU oligomerization by immunoprecipitation and Förster resonance energy transfer (FRET) and characterize a novel protein (MCUb) with two predicted transmembrane domains, 50% sequence similarity and a different expression profile from MCU. Based on computational modelling, MCUb includes critical amino-acid substitutions in the pore region and indeed MCUb does not form a calcium-permeable channel in planar lipid bilayers. In HeLa cells, MCUb is inserted into the oligomer and exerts a dominant-negative effect, reducing the [Ca(2+)]mt increases evoked by agonist stimulation. Accordingly, in vitro co-expression of MCUb with MCU drastically reduces the probability of observing channel activity in planar lipid bilayer experiments. These data unveil the structural complexity of MCU and demonstrate a novel regulatory mechanism, based on the inclusion of dominant-negative subunits in a multimeric channel, that underlies the fine control of the physiologically and pathologically relevant process of mitochondrial calcium homeostasis.

  17. Expression of a dominant negative CELF protein in vivo leads to altered muscle organization, fiber size, and subtype.

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    Dara S Berger

    Full Text Available BACKGROUND: CUG-BP and ETR-3-like factor (CELF proteins regulate tissue- and developmental stage-specific alternative splicing in striated muscle. We previously demonstrated that heart muscle-specific expression of a nuclear dominant negative CELF protein in transgenic mice (MHC-CELFΔ effectively disrupts endogenous CELF activity in the heart in vivo, resulting in impaired cardiac function. In this study, transgenic mice that express the dominant negative protein under a skeletal muscle-specific promoter (Myo-CELFΔ were generated to investigate the role of CELF-mediated alternative splicing programs in normal skeletal muscle. METHODOLOGY/PRINCIPAL FINDINGS: Myo-CELFΔ mice exhibit modest changes in CELF-mediated alternative splicing in skeletal muscle, accompanied by a reduction of endomysial and perimysial spaces, an increase in fiber size variability, and an increase in slow twitch muscle fibers. Weight gain and mean body weight, total number of muscle fibers, and overall muscle strength were not affected. CONCLUSIONS/SIGNIFICANCE: Although these findings demonstrate that CELF activity contributes to the normal alternative splicing of a subset of muscle transcripts in vivo, the mildness of the effects in Myo-CELFΔ muscles compared to those in MHC-CELFΔ hearts suggests CELF activity may be less determinative for alternative splicing in skeletal muscle than in heart muscle. Nonetheless, even these small changes in CELF-mediated splicing regulation were sufficient to alter muscle organization and muscle fiber properties affected in myotonic dystrophy. This lends further evidence to the hypothesis that dysregulation of CELF-mediated alternative splicing programs may be responsible for the disruption of these properties during muscle pathogenesis.

  18. 2-hydroxyglutarate production, but not dominant negative function, is conferred by glioma-derived NADP-dependent isocitrate dehydrogenase mutations.

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    Genglin Jin

    Full Text Available BACKGROUND: Gliomas frequently contain mutations in the cytoplasmic NADP(+-dependent isocitrate dehydrogenase (IDH1 or the mitochondrial NADP(+-dependent isocitrate dehydrogenase (IDH2. Several different amino acid substitutions recur at either IDH1 R132 or IDH2 R172 in glioma patients. Genetic evidence indicates that these mutations share a common gain of function, but it is unclear whether the shared function is dominant negative activity, neomorphic production of (R-2-hydroxyglutarate (2HG, or both. METHODOLOGY/PRINCIPAL FINDINGS: We show by coprecipitation that five cancer-derived IDH1 R132 mutants bind IDH1-WT but that three cancer-derived IDH2 R172 mutants exert minimal binding to IDH2-WT. None of the mutants dominant-negatively lower isocitrate dehydrogenase activity at physiological (40 µM isocitrate concentrations in mammalian cell lysates. In contrast to this, all of these mutants confer 10- to 100-fold higher 2HG production to cells, and glioma tissues containing IDH1 R132 or IDH2 R172 mutations contain high levels of 2HG compared to glioma tissues without IDH mutations (54.4 vs. 0.1 mg 2HG/g protein. CONCLUSIONS: Binding to, or dominant inhibition of, WT IDH1 or IDH2 is not a shared feature of the IDH1 and IDH2 mutations, and thus is not likely to be important in cancer. The fact that the gain of the enzymatic activity to produce 2HG is a shared feature of the IDH1 and IDH2 mutations suggests that this is an important function for these mutants in driving cancer pathogenesis.

  19. Connexin mutation that causes dominant congenital cataracts inhibits gap junctions, but not hemichannels, in a dominant negative manner.

    Science.gov (United States)

    Banks, Eric A; Toloue, Masoud M; Shi, Qian; Zhou, Zifei Jade; Liu, Jialu; Nicholson, Bruce J; Jiang, Jean X

    2009-02-01

    The connexin (Cx) 50, E48K, mutation is associated with a human dominant congenital cataract; however, the underlying molecular mechanism has not been characterized. The glutamate (E) residue at position 48 is highly conserved across animal species and types of connexins. When expressed in paired Xenopus oocytes, human (h) and chicken (ch) Cx50 E48K mutants showed no electrical coupling. In addition, this mutation acts in a dominant negative manner when paired hetero-typically or hetero-merically with wild-type Cx50, but has no such effect on Cx46, the other lens fiber connexin. A similar loss-of-function and dominant negative effect was observed using dye transfer assays in the same system. By using two different dye transfer methods, with two different tracer dyes, we found chCx50 E48K expressed in chicken lens embryonic fibroblast cells by retroviral infection similarly failed to induce dye coupling, and prevented wild-type chCx50 from forming functional gap junctions. In contrast to its effect on gap junctions, the E48K mutation has no effect on hemichannel activity when assayed using electrical conductance in oocytes, and mechanically induced dye uptake in cells. Cx50 is functionally involved in cell differentiation and lens development, and the E48K mutant promotes primary lens cell differentiation indistinguishable from wild-type chCx50, despite its lack of junctional channel function. Together the data show that mutations affecting gap junctions but not hemichannel function of Cx50 can lead to dominant congenital cataracts in humans. This clearly supports the model of intercellular coupling of fiber cells creating a microcirculation of nutrients and metabolites required for lens transparency.

  20. Elevated endogenous expression of the dominant negative basic helix-loop-helix protein ID1 correlates with significant centrosome abnormalities in human tumor cells

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    Gutmann Anja

    2010-01-01

    Full Text Available Abstract Background ID proteins are dominant negative inhibitors of basic helix-loop-helix transcription factors that have multiple functions during development and cellular differentiation. Ectopic (over-expression of ID1 extends the lifespan of primary human epithelial cells. High expression levels of ID1 have been detected in multiple human malignancies, and in some have been correlated with unfavorable clinical prognosis. ID1 protein is localized at the centrosomes and forced (over-expression of ID1 results in errors during centrosome duplication. Results Here we analyzed the steady state expression levels of the four ID-proteins in 18 tumor cell lines and assessed the number of centrosome abnormalities. While expression of ID1, ID2, and ID3 was detected, we failed to detect protein expression of ID4. Expression of ID1 correlated with increased supernumerary centrosomes in most cell lines analyzed. Conclusions This is the first report that shows that not only ectopic expression in tissue culture but endogenous levels of ID1 modulate centrosome numbers. Thus, our findings support the hypothesis that ID1 interferes with centrosome homeostasis, most likely contributing to genomic instability and associated tumor aggressiveness.

  1. Glassy-state stabilization of a dominant negative inhibitor anthrax vaccine containing aluminum hydroxide and glycopyranoside lipid A adjuvants.

    Science.gov (United States)

    Hassett, Kimberly J; Vance, David J; Jain, Nishant K; Sahni, Neha; Rabia, Lilia A; Cousins, Megan C; Joshi, Sangeeta; Volkin, David B; Middaugh, C Russell; Mantis, Nicholas J; Carpenter, John F; Randolph, Theodore W

    2015-02-01

    During transport and storage, vaccines may be exposed to temperatures outside of the range recommended for storage, potentially causing efficacy losses. To better understand and prevent such losses, dominant negative inhibitor (DNI), a recombinant protein antigen for a candidate vaccine against anthrax, was formulated as a liquid and as a glassy lyophilized powder with the adjuvants aluminum hydroxide and glycopyranoside lipid A (GLA). Freeze-thawing of the liquid vaccine caused the adjuvants to aggregate and decreased its immunogenicity in mice. Immunogenicity of liquid vaccines also decreased when stored at 40°C for 8 weeks, as measured by decreases in neutralizing antibody titers in vaccinated mice. Concomitant with efficacy losses at elevated temperatures, changes in DNI structure were detected by fluorescence spectroscopy and increased deamidation was observed by capillary isoelectric focusing (cIEF) after only 1 week of storage of the liquid formulation at 40°C. In contrast, upon lyophilization, no additional deamidation after 4 weeks at 40°C and no detectable changes in DNI structure or reduction in immunogenicity after 16 weeks at 40°C were observed. Vaccines containing aluminum hydroxide and GLA elicited higher immune responses than vaccines adjuvanted with only aluminum hydroxide, with more mice responding to a single dose.

  2. Expression of a mutant form of cellulose synthase AtCesA7 causes dominant negative effect on cellulose biosynthesis.

    Science.gov (United States)

    Zhong, Ruiqin; Morrison, W Herbert; Freshour, Glenn D; Hahn, Michael G; Ye, Zheng-Hua

    2003-06-01

    Cellulose synthase catalytic subunits (CesAs) have been implicated in catalyzing the biosynthesis of cellulose, the major component of plant cell walls. Interactions between CesA subunits are thought to be required for normal cellulose synthesis, which suggests that incorporation of defective CesA subunits into cellulose synthase complex could potentially cause a dominant effect on cellulose synthesis. However, all CesA mutants so far reported have been shown to be recessive in terms of cellulose synthesis. In the course of studying the molecular mechanisms regulating secondary wall formation in fibers, we have found that a mutant allele of AtCesA7 gene in the fra5 (fragile fiber 5) mutant causes a semidominant phenotype in the reduction of fiber cell wall thickness and cellulose content. The fra5 missense mutation occurred in a conserved amino acid located in the second cytoplasmic domain of AtCesA7. Overexpression of the fra5 mutant cDNA in wild-type plants not only reduced secondary wall thickness and cellulose content but also decreased primary wall thickness and cell elongation. In contrast, overexpression of the fra6 mutant form of AtCesA8 did not cause any reduction in cell wall thickness and cellulose content. These results suggest that the fra5 mutant protein may interfere with the function of endogenous wild-type CesA proteins, thus resulting in a dominant negative effect on cellulose biosynthesis.

  3. A novel human aquaporin-4 splice variant exhibits a dominant-negative activity: a new mechanism to regulate water permeability.

    Science.gov (United States)

    De Bellis, Manuela; Pisani, Francesco; Mola, Maria Grazia; Basco, Davide; Catalano, Francesco; Nicchia, Grazia Paola; Svelto, Maria; Frigeri, Antonio

    2014-02-01

    Two major isoforms of aquaporin-4 (AQP4) have been described in human tissue. Here we report the identification and functional analysis of an alternatively spliced transcript of human AQP4, AQP4-Δ4, that lacks exon 4. In transfected cells AQP4-Δ4 is mainly retained in the endoplasmic reticulum and shows no water transport properties. When AQP4-Δ4 is transfected into cells stably expressing functional AQP4, the surface expression of the full-length protein is reduced. Furthermore, the water transport activity of the cotransfectants is diminished in comparison to transfectants expressing only AQP4. The observed down-regulation of both the expression and water channel activity of AQP4 is likely to originate from a dominant-negative effect caused by heterodimerization between AQP4 and AQP4-Δ4, which was detected in coimmunoprecipitation studies. In skeletal muscles, AQP4-Δ4 mRNA expression inversely correlates with the level of AQP4 protein and is physiologically associated with different types of skeletal muscles. The expression of AQP4-Δ4 may represent a new regulatory mechanism through which the cell-surface expression and therefore the activity of AQP4 can be physiologically modulated.

  4. Cord blood natural killer cells expressing a dominant negative TGF-β receptor: Implications for adoptive immunotherapy for glioblastoma.

    Science.gov (United States)

    Yvon, Eric S; Burga, Rachel; Powell, Allison; Cruz, Conrad R; Fernandes, Rohan; Barese, Cecilia; Nguyen, Tuongvan; Abdel-Baki, Mohamed S; Bollard, Catherine M

    2017-03-01

    Cord blood (CB) natural killer (NK) cells are promising effector cells for tumor immunotherapy but are currently limited by immune-suppressive cytokines in the tumor microenvironment, such as transforming growth factor (TGF-β). We observed that TGF-β inhibits expression of activating receptors such as NKG2D and DNAM1 and decreases killing activity against glioblastoma tumor cells through inhibition of perforin secretion. To overcome the detrimental effects of TGF-β, we engrafted a dominant negative TGF-β receptor II (DNRII) on CB-derived NK cells by retroviral transduction and evaluated their ability to kill glioblastoma cells in the presence of TGF-β. After manufacture using Good Manufacturing Practice-compliant methodologies and transduction with DNRII, CB-derived DNRII-transduced NK cells expanded to clinically relevant numbers and retained both their killing ability and their secretion of interferon-γ upon activation. More important, these cells maintained both perforin expression and NKG2D/DNMA1 expression in the presence of TGF-β allowing for recognition and killing of glioblastoma tumor cells. Hence, NK cells expressing a DNRII should have a functional advantage over unmodified NK cells in the presence of TGF-β-secreting tumors and may be an important therapeutic approach for patients with cancer. Copyright © 2017 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

  5. [Effect of dominant negative HIF-1alpha (dn HIF-1alpha) on biological characteristics of uterine cervix cancer cells].

    Science.gov (United States)

    Tang, Bin-Zhi; Zhao, Feng-Yan; Wei, Ting; Mu, De-Zhi; Mao, Meng; Fu, Qiang; Zhang, Lin; Qu, Yia

    2008-05-01

    To explore the effect of dominant negative HIF-1alpha (dn HIF-1alpha) on biological characteristics of uterine cervix cancer cell SiHa and elucidate the related mechanism. pcDNA3. 1-dn HIF-1alpha was transfected into SiHa cells. The expression of HIF-1alpha and VEGF protein were detected by immunocytochemical method and Western Blotting. The growth proliferation of cells was surveyed by the MTT assay and cell apoptosis was detected through TUNEL after treated with CoCl2, meanwhile the results were compared with the group transfected with mock plasmid and untransfected group. After successfully transfected with relevant plasmid, there's no obvious difference of expression of HIF-1alpha among dn HIF-1alpha group, pcDNA3. 1 group, and untransfected group, however the expression of VEGF of dn HIF-1alpha group was significantly lower than that of the others (P dn HIF-1alpha group was obviously lower than that of the other two (P dn HIF-1alpha group among these three (P < 0.05). Domain negative HIF-1alpha can inhibit the proliferation of uterine cervix cancer cell and accelerate its apoptosis under hypoxia induced by CoCl2, as well as decrease the expression of VEGF protein. The implications of all this were that the domain negative HIF-1alpha may play an important role in the therapy of uterine cervix cancer.

  6. Progression of mouse skin carcinogenesis is associated with increased ERα levels and is repressed by a dominant negative form of ERα.

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    Stella Logotheti

    Full Text Available Estrogen receptors (ER, namely ERα and ERβ, are hormone-activated transcription factors with an important role in carcinogenesis. In the present study, we aimed at elucidating the implication of ERα in skin cancer, using chemically-induced mouse skin tumours, as well as cell lines representing distinct stages of mouse skin oncogenesis. First, using immunohistochemical staining we showed that ERα is markedly increased in aggressive mouse skin tumours in vivo as compared to the papilloma tumours, whereas ERβ levels are low and become even lower in the aggressive spindle tumours of carcinogen-treated mice. Then, using the multistage mouse skin carcinogenesis model, we showed that ERα gradually increases during promotion and progression stages of mouse skin carcinogenesis, peaking at the most aggressive stage, whereas ERβ levels only slightly change throughout skin carcinogenesis. Stable transfection of the aggressive, spindle CarB cells with a dominant negative form of ERα (dnERα resulted in reduced ERα levels and reduced binding to estrogen responsive elements (ERE-containing sequences. We characterized two highly conserved EREs on the mouse ERα promoter through which dnERα decreased endogenous ERα levels. The dnERα-transfected CarB cells presented altered protein levels of cytoskeletal and cell adhesion molecules, slower growth rate and impaired anchorage-independent growth in vitro, whereas they gave smaller tumours with extended latency period of tumour onset in vivo. Our findings suggest an implication of ERα in the aggressiveness of spindle mouse skin cancer cells, possibly through regulation of genes affecting cell shape and adhesion, and they also provide hints for the effective targeting of spindle cancer cells by dnERα.

  7. Increased adipogenesis in cultured embryonic chondrocytes and in adult bone marrow of dominant negative Erg transgenic mice.

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    Sébastien Flajollet

    Full Text Available In monolayer culture, primary articular chondrocytes have an intrinsic tendency to lose their phenotype during expansion. The molecular events underlying this chondrocyte dedifferentiation are still largely unknown. Several transcription factors are important for chondrocyte differentiation. The Ets transcription factor family may be involved in skeletal development. One family member, the Erg gene, is mainly expressed during cartilage formation. To further investigate the potential role of Erg in the maintenance of the chondrocyte phenotype, we isolated and cultured chondrocytes from the rib cartilage of embryos of transgenic mice that express a dominant negative form of Erg (DN-Erg during cartilage formation. DN-Erg expression in chondrocytes cultured for up to 20 days did not affect the early dedifferentiation usually observed in cultured chondrocytes. However, lipid droplets accumulated in DN-Erg chondrocytes, suggesting adipocyte emergence. Transcriptomic analysis using a DNA microarray, validated by quantitative RT-PCR, revealed strong differential gene expression, with a decrease in chondrogenesis-related markers and an increase in adipogenesis-related gene expression in cultured DN-Erg chondrocytes. These results indicate that Erg is involved in either maintaining the chondrogenic phenotype in vitro or in cell fate orientation. Along with the in vitro studies, we compared adipocyte presence in wild-type and transgenic mice skeletons. Histological investigations revealed an increase in the number of adipocytes in the bone marrow of adult DN-Erg mice even though no adipocytes were detected in embryonic cartilage or bone. These findings suggest that the Ets transcription factor family may contribute to the homeostatic balance in skeleton cell plasticity.

  8. Impaired inflammatory pain and thermal hyperalgesia in mice expressing neuron-specific dominant negative mitogen activated protein kinase kinase (MEK

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    Kaplan David

    2006-01-01

    Full Text Available Abstract Background Numerous studies have implicated spinal extracellular signal-regulated kinases (ERKs as mediators of nociceptive plasticity. These studies have utilized pharmacological inhibition of MEK to demonstrate a role for ERK signaling in pain, but this approach cannot distinguish between effects of ERK in neuronal and non-neuronal cells. The present studies were undertaken to test the specific role of neuronal ERK in formalin-induced inflammatory pain. Dominant negative MEK (DN MEK mutant mice in which MEK function is suppressed exclusively in neurons were tested in the formalin model of inflammatory pain. Results Formalin-induced second phase spontaneous pain behaviors as well as thermal hyperalgesia measured 1 – 3 hours post-formalin were significantly reduced in the DN MEK mice when compared to their wild type littermate controls. In addition, spinal ERK phosphorylation following formalin injection was significantly reduced in the DN MEK mice. This was not due to a reduction of the number of unmyelinated fibers in the periphery, since these were almost double the number observed in wild type controls. Further examination of the effects of suppression of MEK function on a downstream target of ERK phosphorylation, the A-type potassium channel, showed that the ERK-dependent modulation of the A-type currents is significantly reduced in neurons from DN MEK mice compared to littermate wild type controls. Conclusion Our results demonstrate that the neuronal MEK-ERK pathway is indeed an important intracellular cascade that is associated with formalin-induced inflammatory pain and thermal hyperalgesia.

  9. Effect of selective expression of dominant-negative PPARγ in pro-opiomelanocortin neurons on the control of energy balance.

    Science.gov (United States)

    Stump, Madeliene; Guo, Deng-Fu; Lu, Ko-Ting; Mukohda, Masashi; Liu, Xuebo; Rahmouni, Kamal; Sigmund, Curt D

    2016-07-01

    Peroxisome proliferator-activated receptor-γ (PPARγ), a master regulator of adipogenesis, was recently shown to affect energy homeostasis through its actions in the brain. Deletion of PPARγ in mouse brain, and specifically in the pro-opiomelanocortin (POMC) neurons, results in resistance to diet-induced obesity. To study the mechanisms by which PPARγ in POMC neurons controls energy balance, we constructed a Cre-recombinase-dependent conditionally activatable transgene expressing either wild-type (WT) or dominant-negative (P467L) PPARγ and the tdTomato reporter. Inducible expression of both forms of PPARγ was validated in cells in culture, in liver of mice infected with an adenovirus expressing Cre-recombinase (AdCre), and in the brain of mice expressing Cre-recombinase either in all neurons (NES(Cre)/PPARγ-P467L) or selectively in POMC neurons (POMC(Cre)/PPARγ-P467L). Whereas POMC(Cre)/PPARγ-P467L mice exhibited a normal pattern of weight gain when fed 60% high-fat diet, they exhibited increased weight gain and fat mass accumulation in response to a 10% fat isocaloric-matched control diet. POMC(Cre)/PPARγ-P467L mice were leptin sensitive on control diet but became leptin resistant when fed 60% high-fat diet. There was no difference in body weight between POMC(Cre)/PPARγ-WT mice and controls in response to 60% high-fat diet. However, POMC(Cre)/PPARγ-WT, but not POMC(Cre)/PPARγ-P467L, mice increased body weight in response to rosiglitazone, a PPARγ agonist. These observations support the concept that alterations in PPARγ-driven mechanisms in POMC neurons can play a role in the regulation of metabolic homeostasis under certain dietary conditions.

  10. A dominant negative ERβ splice variant determines the effectiveness of early or late estrogen therapy after ovariectomy in rats.

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    Jun Ming Wang

    Full Text Available The molecular mechanisms for the discrepancy in outcome of initiating estrogen therapy (ET around peri-menopause or several years after menopause in women are unknown. We hypothesize that the level of expression of a dominant negative estrogen receptor (ER β variant, ERβ2, may be a key factor determining the effectiveness of ET in post-menopausal women. We tested this hypothesis in ovariectomized nine month-old (an age when irregular estrous cycles occur female Sprague Dawley rats. Estradiol treatment was initiated either 6 days (Early ET, analogous to 4 months post-menopause in humans, or 180 days (Late ET, analogous to 11 years post-menopause in humans after ovariectomy. Although ERβ2 expression increased in all OVX rats, neurogenic and neuroprotective responses to estradiol differed in Early and Late ET. Early ET reduced ERβ2 expression in both hippocampus and white blood cells, increased the hippocampal cell proliferation as assessed by Ki-67 expression, and improved mobility in the forced swim test. Late ET resulted in either no or modest effects on these parameters. There was a close correlation between the degree of ERβ2 expression and the preservation of neural effects by ET after OVX in rats, supporting the hypothesis that persistent elevated levels of ERβ2 are a molecular basis for the diminished effectiveness of ET in late post-menopausal women. The correlation between the expression of ERβ2 in circulating white blood cells and brain cells suggests that ERβ2 expression in peripheral blood cells may be an easily accessible marker to predict the effective window for ET in the brain.

  11. Dominant-negative androgen receptor inhibition of intracrine androgen-dependent growth of castration-recurrent prostate cancer.

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    Mark A Titus

    Full Text Available BACKGROUND: Prostate cancer (CaP is the second leading cause of cancer death in American men. Androgen deprivation therapy is initially effective in CaP treatment, but CaP recurs despite castrate levels of circulating androgen. Continued expression of the androgen receptor (AR and its ligands has been linked to castration-recurrent CaP growth. PRINCIPAL FINDING: In this report, the ligand-dependent dominant-negative ARΔ142-337 (ARΔTR was expressed in castration-recurrent CWR-R1 cell and tumor models to elucidate the role of AR signaling. Expression of ARΔTR decreased CWR-R1 tumor growth in the presence and absence of exogenous testosterone (T and improved survival in the presence of exogenous T. There was evidence for negative selection of ARΔTR transgene in T-treated mice. Mass spectrometry revealed castration-recurrent CaP dihydrotestosterone (DHT levels sufficient to activate AR and ARΔTR. In the absence of exogenous testosterone, CWR-R1-ARΔTR and control cells exhibited altered androgen profiles that implicated epithelial CaP cells as a source of intratumoral AR ligands. CONCLUSION: The study provides in vivo evidence that activation of AR signaling by intratumoral AR ligands is required for castration-recurrent CaP growth and that epithelial CaP cells produce sufficient active androgens for CaP recurrence during androgen deprivation therapy. Targeting intracrine T and DHT synthesis should provide a mechanism to inhibit AR and growth of castration-recurrent CaP.

  12. In vivo Modeling Implicates APOL1 in Nephropathy: Evidence for Dominant Negative Effects and Epistasis under Anemic Stress.

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    Blair R Anderson

    2015-07-01

    Full Text Available African Americans have a disproportionate risk for developing nephropathy. This disparity has been attributed to coding variants (G1 and G2 in apolipoprotein L1 (APOL1; however, there is little functional evidence supporting the role of this protein in renal function. Here, we combined genetics and in vivo modeling to examine the role of apol1 in glomerular development and pronephric filtration and to test the pathogenic potential of APOL1 G1 and G2. Translational suppression or CRISPR/Cas9 genome editing of apol1 in zebrafish embryos results in podocyte loss and glomerular filtration defects. Complementation of apol1 morphants with wild-type human APOL1 mRNA rescues these defects. However, the APOL1 G1 risk allele does not ameliorate defects caused by apol1 suppression and the pathogenicity is conferred by the cis effect of both individual variants of the G1 risk haplotype (I384M/S342G. In vivo complementation studies of the G2 risk allele also indicate that the variant is deleterious to protein function. Moreover, APOL1 G2, but not G1, expression alone promotes developmental kidney defects, suggesting a possible dominant-negative effect of the altered protein. In sickle cell disease (SCD patients, we reported previously a genetic interaction between APOL1 and MYH9. Testing this interaction in vivo by co-suppressing both transcripts yielded no additive effects. However, upon genetic or chemical induction of anemia, we observed a significantly exacerbated nephropathy phenotype. Furthermore, concordant with the genetic interaction observed in SCD patients, APOL1 G2 reduces myh9 expression in vivo, suggesting a possible interaction between the altered APOL1 and myh9. Our data indicate a critical role for APOL1 in renal function that is compromised by nephropathy-risk encoding variants. Moreover, our interaction studies indicate that the MYH9 locus is also relevant to the phenotype in a stressed microenvironment and suggest that consideration of

  13. Heterozygous PINK1 p.G411S increases risk of Parkinson's disease via a dominant-negative mechanism.

    Science.gov (United States)

    Puschmann, Andreas; Fiesel, Fabienne C; Caulfield, Thomas R; Hudec, Roman; Ando, Maya; Truban, Dominika; Hou, Xu; Ogaki, Kotaro; Heckman, Michael G; James, Elle D; Swanberg, Maria; Jimenez-Ferrer, Itzia; Hansson, Oskar; Opala, Grzegorz; Siuda, Joanna; Boczarska-Jedynak, Magdalena; Friedman, Andrzej; Koziorowski, Dariusz; Aasly, Jan O; Lynch, Timothy; Mellick, George D; Mohan, Megha; Silburn, Peter A; Sanotsky, Yanosh; Vilariño-Güell, Carles; Farrer, Matthew J; Chen, Li; Dawson, Valina L; Dawson, Ted M; Wszolek, Zbigniew K; Ross, Owen A; Springer, Wolfdieter

    2017-01-01

    by a partial dominant-negative function phenotype.

  14. Surface expression and limited proteolysis of ADAM10 are increased by a dominant negative inhibitor of dynamin

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    Slack Barbara E

    2011-05-01

    Full Text Available Abstract Background The amyloid precursor protein (APP is cleaved by β- and γ-secretases to generate toxic amyloid β (Aβ peptides. Alternatively, α-secretases cleave APP within the Aβ domain, precluding Aβ formation and releasing the soluble ectodomain, sAPPα. We previously showed that inhibition of the GTPase dynamin reduced APP internalization and increased release of sAPPα, apparently by prolonging the interaction between APP and α-secretases at the plasma membrane. This was accompanied by a reduction in Aβ generation. In the present study, we investigated whether surface expression of the α-secretase ADAM (a disintegrin and metalloprotease10 is also regulated by dynamin-dependent endocytosis. Results Transfection of human embryonic kidney (HEK cells stably expressing M3 muscarinic receptors with a dominant negative dynamin I mutant (dyn I K44A, increased surface expression of both immature, and mature, catalytically active forms of co-expressed ADAM10. Surface levels of ADAM10 were unaffected by activation of protein kinase C (PKC or M3 receptors, indicating that receptor-coupled shedding of the ADAM substrate APP is unlikely to be mediated by inhibition of ADAM10 endocytosis in this cell line. Dyn I K44A strongly increased the formation of a C-terminal fragment of ADAM10, consistent with earlier reports that the ADAM10 ectodomain is itself a target for sheddases. The abundance of this fragment was increased in the presence of a γ-secretase inhibitor, but was not affected by M3 receptor activation. The dynamin mutant did not affect the distribution of ADAM10 and its C-terminal fragment between raft and non-raft membrane compartments. Conclusions Surface expression and limited proteolysis of ADAM10 are regulated by dynamin-dependent endocytosis, but are unaffected by activation of signaling pathways that upregulate shedding of ADAM substrates such as APP. Modulation of ADAM10 internalization could affect cellular behavior in two

  15. The β1-subunit of Na(v1.5 cardiac sodium channel is required for a dominant negative effect through α-α interaction.

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    Aurélie Mercier

    Full Text Available Brugada syndrome (BrS is an inherited autosomal dominant cardiac channelopathy. Several mutations on the cardiac sodium channel Na(v1.5 which are responsible for BrS lead to misfolded proteins that do not traffic properly to the plasma membrane. In order to mimic patient heterozygosity, a trafficking defective mutant, R1432G was co-expressed with Wild Type (WT Na(v1.5 channels in HEK293T cells. This mutant significantly decreased the membrane Na current density when it was co-transfected with the WT channel. This dominant negative effect did not result in altered biophysical properties of Na(v1.5 channels. Luminometric experiments revealed that the expression of mutant proteins induced a significant reduction in membrane expression of WT channels. Interestingly, we have found that the auxiliary Na channel β(1-subunit was essential for this dominant negative effect. Indeed, the absence of the β(1-subunit prevented the decrease in WT sodium current density and surface proteins associated with the dominant negative effect. Co-immunoprecipitation experiments demonstrated a physical interaction between Na channel α-subunits. This interaction occurred only when the β(1-subunit was present. Our findings reveal a new role for β(1-subunits in cardiac voltage-gated sodium channels by promoting α-α subunit interaction which can lead to a dominant negative effect when one of the α-subunits shows a trafficking defective mutation.

  16. Altered promoter recycling rates contribute to dominant-negative activity of human peroxisome proliferator-activated receptor-gamma mutations associated with diabetes.

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    Li, Gang; Leff, Todd

    2007-04-01

    The transcription factor peroxisome proliferator-activated receptor-gamma (PPARgamma) plays an important role in regulating lipid and glucose metabolism and improves insulin sensitivity in diabetic patients when activated by thiazolidinedione drugs. Several loss-of-function mutations in PPARgamma have been identified that cause lipodystrophy and diabetes in humans. Because affected individuals are heterozygotes and have one normal PPARgamma allele, it is of interest to know whether these mutations act in a dominant-negative fashion to inhibit the activity of the wild-type (WT) receptor. Here we compare the molecular phenotypes of two previously identified PPARgamma mutations: P467L, reported to be dominant negative; and F388L, reported to be devoid of dominant-negative activity. We developed a competitive chromatin immunoprecipitation assay to measure the relative ability of mutant PPARgamma to compete with WT receptor for binding to a PPAR regulatory element (PPRE)-containing promoter. By determining the ratio of mutant and WT receptors bound to a PPRE over time, we estimated the relative promoter turnover rate of each receptor. This assay demonstrated that PPARgamma bearing the P467L had a reduced promoter turnover rate compared with the F388L receptor, and over time out-competed the WT receptor for promoter binding sites. We propose that the P467L receptor is dominant negative because in a cell containing both WT and mutant receptors, the majority of the PPAR-regulated promoters will be occupied by the transcriptionally defective mutant receptor. In contrast, the F388L mutation lacks dominant-negative activity because its more rapid promoter turnover rate prevented it from out-competing the WT receptor for promoter binding sites.

  17. Dominant negative mutants of Bacillus thuringiensis Cry1Ab toxin function as anti-toxins: demonstration of the role of oligomerization in toxicity.

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    Claudia Rodríguez-Almazán

    Full Text Available BACKGROUND: Bacillus thuringiensis Cry toxins, that are used worldwide in insect control, kill insects by a mechanism that depends on their ability to form oligomeric pores that insert into the insect-midgut cells. These toxins are being used worldwide in transgenic plants or spray to control insect pests in agriculture. However, a major concern has been the possible effects of these insecticidal proteins on non-target organisms mainly in ecosystems adjacent to agricultural fields. METHODOLOGY/PRINCIPAL FINDINGS: We isolated and characterized 11 non-toxic mutants of Cry1Ab toxin affected in different steps of the mechanism of action namely binding to receptors, oligomerization and pore-formation. These mutant toxins were analyzed for their capacity to block wild type toxin activity, presenting a dominant negative phenotype. The dominant negative phenotype was analyzed at two levels, in vivo by toxicity bioassays against susceptible Manduca sexta larvae and in vitro by pore formation activity in black lipid bilayers. We demonstrate that some mutations located in helix alpha-4 completely block the wild type toxin activity at sub-stoichiometric level confirming a dominant negative phenotype, thereby functioning as potent antitoxins. CONCLUSIONS/SIGNIFICANCE: This is the first reported case of a Cry toxin dominant inhibitor. These data demonstrate that oligomerization is a fundamental step in Cry toxin action and represent a potential mechanism to protect special ecosystems from the possible effect of Cry toxins on non-target organisms.

  18. Missense mutations in pyruvate kinase M2 promote cancer metabolism, oxidative endurance, anchorage independence, and tumor growth in a dominant negative manner.

    Science.gov (United States)

    Iqbal, Mohd Askandar; Siddiqui, Farid Ahmad; Chaman, Noor; Gupta, Vibhor; Kumar, Bhupender; Gopinath, Prakasam; Bamezai, Rameshwar N K

    2014-03-21

    The present study was designed to examine the functional relevance of two heterozygous mutations (H391Y and K422R), observed earlier by us in the Bloom syndrome condition. Cells stably expressing exogenous wild-type or mutant PKM2 (K422R or H391Y) or co-expressing both wild type and mutant (PKM2-K422R or PKM2-H391Y) were assessed for cancer metabolism and tumorigenic potential. Interestingly, cells co-expressing PKM2 and mutant (K422R or H391Y) showed significantly aggressive cancer metabolism as compared with cells expressing either wild-type or mutant PKM2 independently. A similar trend was observed for oxidative endurance, tumorigenic potential, cellular proliferation, and tumor growth. These observations signify the dominant negative nature of mutations. Remarkably, PKM2-H391Y co-expressed cells showed a maximal effect on all the studied parameters. Such a dominant negative impaired function of PKM2 in tumor development is not known; this study demonstrates for the first time the possible predisposition of Bloom syndrome patients with impaired PKM2 activity to cancer and the importance of studying genetic variations in PKM2 in the future to understand their relevance in cancer in general.

  19. OVEREXPRESSION OF DOMINANT-NEGATIVE IRE1 ENZYME IN H1299-shE6AP CELLS INCREASES HEAT SHOCK ELEMENT-DEPENDENT TRANSCRIPTION

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    D. O.

    2015-12-01

    Full Text Available To investigate IRE1-dependent branch of endoplasmic reticulum stress pathway in various cancer cells we created cDNA-constructs for expression of dominant-negative inositol-requiring enzyme – 1 IRE1 and cytosolic domain of IRE1 fused on a C-terminus with c-Myc and 6xHis tags. The non-small-cell lung carcinoma cells H1299-shE6AP were transfected with these constructs. Using anti-c-Myc antibodies we demonstrated effective, dose-dependent expression of dominant-negative and cytosolic IRE1 proteins. In order to investigate IRE1-mediated, heat shock element-dependent transcription, the cells were further transfected with a reporter construct containing heat shock element. We observed that overexpression of dnIRE1 in H1299-shE6AP cells led to significant induction of heat shock element-dependent transcription. This observation may reflect the induction of heat shock genes, which contribute to cellular adaptation to inhibition of native IRE1, a key sensory-signaling enzyme of endoplasmic reticulum stress pathway, which suppresses cancer cell proliferative capacities and alternates the expression of numerous genes, including many transcription factors.

  20. The new and recurrent FLT3 juxtamembrane deletion mutation shows a dominant negative effect on the wild-type FLT3 receptor

    Science.gov (United States)

    Sandhöfer, Nadine; Bauer, Julia; Reiter, Katrin; Dufour, Annika; Rothenberg, Maja; Konstandin, Nikola P.; Zellmeier, Evelyn; Tizazu, Belay; Greif, Philipp A.; Metzeler, Klaus H.; Hiddemann, Wolfgang; Polzer, Harald; Spiekermann, Karsten

    2016-01-01

    In acute myeloid leukemia (AML), the Fms-like tyrosine kinase 3 (FLT3) is one of the most frequently mutated genes. Recently, a new and recurrent juxtamembrane deletion mutation (p.Q569Vfs*2) resulting in a truncated receptor was identified. The mutated receptor is expressed on the cell surface and still binds its ligand but loses the ability to activate ERK signaling. FLT3 p.Q569fs-expressing Ba/F3 cells show no proliferation after ligand stimulation. Furthermore, coexpressed with the FLT3 wild-type (WT) receptor, the truncated receptor suppresses stimulation and activation of the WT receptor. Thus, FLT3 p.Q569Vfs*2, to our knowledge, is the first FLT3 mutation with a dominant negative effect on the WT receptor. PMID:27346558

  1. The new and recurrent FLT3 juxtamembrane deletion mutation shows a dominant negative effect on the wild-type FLT3 receptor.

    Science.gov (United States)

    Sandhöfer, Nadine; Bauer, Julia; Reiter, Katrin; Dufour, Annika; Rothenberg, Maja; Konstandin, Nikola P; Zellmeier, Evelyn; Tizazu, Belay; Greif, Philipp A; Metzeler, Klaus H; Hiddemann, Wolfgang; Polzer, Harald; Spiekermann, Karsten

    2016-06-27

    In acute myeloid leukemia (AML), the Fms-like tyrosine kinase 3 (FLT3) is one of the most frequently mutated genes. Recently, a new and recurrent juxtamembrane deletion mutation (p.Q569Vfs*2) resulting in a truncated receptor was identified. The mutated receptor is expressed on the cell surface and still binds its ligand but loses the ability to activate ERK signaling. FLT3 p.Q569fs-expressing Ba/F3 cells show no proliferation after ligand stimulation. Furthermore, coexpressed with the FLT3 wild-type (WT) receptor, the truncated receptor suppresses stimulation and activation of the WT receptor. Thus, FLT3 p.Q569Vfs*2, to our knowledge, is the first FLT3 mutation with a dominant negative effect on the WT receptor.

  2. A dominant negative mutant of rab5 inhibits infection of cells by foot-and-mouth disease virus; implications for virus entry

    DEFF Research Database (Denmark)

    Johns, Helen; Berryman, Stephen; Monaghan, Paul;

    2009-01-01

    is also dependent on clathrin-mediate endocytosis and an acidic pH within endosomes. Also, the effect on FMDV infection of dominant-negative (DN) mutants of cellular rab proteins that regulate endosomal traffic was examined. Expression of DN rab5 reduced the number of FMDV-infected cells by 80%, while......Foot-and-mouth disease virus (FMDV) can use a number of different integrins (alphavβ1, alphavβ3, alphavβ6, and alphavβ8) as receptors to initiate infection. Infection mediated by alphavβ6 is known to occur by clathrin-mediated endocytosis and is dependent on the acidic pH within endosomes...

  3. Small Ubiquitin-Like Modifier Conjugating Enzyme with Active Site Mutation Acts as Dominant Negative Inhibitor of SUMO Conjugation in Arabidopsis

    Institute of Scientific and Technical Information of China (English)

    Konstantin Tomanov; Christian Hardtke; Ruchika Budhiraja; Rebecca Hermkes; George Coupland; Andreas Bachmair

    2013-01-01

    Small ubiquitin-like modifier (SUMO) conjugation affects a broad range of processes in plants,including growth,flower initiation,pathogen defense,and responses to abiotic stress.Here,we investigate in vivo and in vitro a SUMO conjugating enzyme with a Cys to Ser change in the active site,and show that it has a dominant negative effect.In planta expression significantly perturbs normal development,leading to growth retardation,early flowering and gene expression changes.We suggest that the mutant protein can serve as a probe to investigate sumoylation,also in plants for which poor genetic infrastructure precludes analysis via loss-of-function mutants.

  4. A cell-permeable dominant-negative survivin protein induces apoptosis and sensitizes prostate cancer cells to TNF-α therapy

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    Kanwar Jagat R

    2010-10-01

    Full Text Available Abstract Background Survivin is a member of the inhibitor-of-apoptosis (IAP family which is widely expressed by many different cancers. Overexpression of survivin is associated with drug resistance in cancer cells, and reduced patient survival after chemotherapy and radiotherapy. Agents that antagonize the function of survivin hold promise for treating many forms of cancer. The purpose of this study was to investigate whether a cell-permeable dominant-negative survivin protein would demonstrate bioactivity against prostate and cervical cancer cells grown in three dimensional culture. Results A dominant-negative survivin (C84A protein fused to the cell penetrating peptide poly-arginine (R9 was expressed in E. coli and purified by affinity chromatography. Western blot analysis revealed that dNSurR9-C84A penetrated into 3D-cultured HeLa and DU145 cancer cells, and a cell viability assay revealed it induced cancer cell death. It increased the activities of caspase-9 and caspase-3, and rendered DU145 cells sensitive to TNF-α via by a mechanism involving activation of caspase-8. Conclusions The results demonstrate that antagonism of survivin function triggers the apoptosis of prostate and cervical cancer cells grown in 3D culture. It renders cancer cells sensitive to the proapoptotic affects of TNF-α, suggesting that survivin blocks the extrinsic pathway of apoptosis. Combination of the biologically active dNSurR9-C84A protein or other survivin antagonists with TNF-α therapy warrants consideration as an approach to cancer therapy.

  5. Mdm2 ligase dead mutants did not act in a dominant negative manner to re-activate p53, but promoted tumor cell growth.

    Science.gov (United States)

    Swaroop, Manju; Sun, Yi

    2003-01-01

    Mdm2 (murine double minute 2) is an oncogene, first identified in BALB/c 3T3 cells. Over-expression and gene amplification of Mdm2 were found in a variety of human cancers. Recently, Mdm2 was found to be an E3 ubiquitin ligase that promotes degradation of p53, which contributes significantly to its oncogenic activity. In this study, we test a hypothesis that Mdm2 ligase dead mutants, which retained p53 binding activity but lost degradation activity, would act in a dominant negative manner to re-activate p53, especially upon stressed conditions. Five Mdm2 constructs expressing wild-type and E3 ligase-dead Mdm2 proteins were generated in a Tet-Off system and transfected into MCF-7 breast cancer cells (p53+/+ with Mdm2 overexpression) as well as MCF10A immortalized breast cells (p53+/+ without Mdm2 overexpression) as a normal control. We found that expression of Mdm2 mutants were tightly regulated by doxycycline. Withdrawal of doxycycline in culture medium triggered overexpression of Mdm2 mutants. However, expression of ligase dead mutants in MCF7 and MCF10A cells did not reactivate p53 as shown by a luciferase-reporter transcription assay and Western blot of p53 and its downstream target p21 under either unstressed condition or after exposure to DNA damaging agents. Biologically, over-expression of Mdm2 mutants had no effect on p53-induced apoptosis following DNA damage. Interestingly, over-expression of Mdm2 mutants promoted growth of MCF7 tumor cells probably via a p53-independent mechanism. Over-expression of Mdm2 mutants, however, had no effect on the growth of normal MCF10A cells and did not cause their transformation. Thus, ligase dead mutants of Mdm2 did not act in a dominant negative manner to reactivate p53 and they are not oncogenes in MCF10A cells.

  6. A dominant negative form of inositol 1,4,5-trisphosphate receptor induces metacyclogenesis and increases mitochondrial density in Trypanosoma cruzi

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    Hashimoto, Muneaki, E-mail: muneaki@juntendo.ac.jp [Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421 (Japan); Nara, Takeshi, E-mail: tnara@juntendo.ac.jp [Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421 (Japan); Enomoto, Masahiro, E-mail: menomoto@uhnres.utoronto.ca [Laboratory for Developmental Neurobiology, RIKEN Brain Science Institute, Saitama, 351-0198 (Japan); Princess Margaret Cancer Centre, Department of Medical Biophysics, University of Toronto, M5G1L7, Toronto, Ontario (Canada); Kurebayashi, Nagomi, E-mail: nagomik@juntendo.ac.jp [Department of Pharmacology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, 113-8421 (Japan); Yoshida, Mitsutaka, E-mail: myoshida@juntendo.ac.jp [Laboratoly of Morphology and Image Analysis, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421 (Japan); Sakurai, Takashi, E-mail: tsakurai@juntendo.ac.jp [Department of Pharmacology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, 113-8421 (Japan); Mita, Toshihiro, E-mail: tmita@juntendo.ac.jp [Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421 (Japan); Mikoshiba, Katsuhiko, E-mail: mikosiba@brain.riken.jp [Laboratory for Developmental Neurobiology, RIKEN Brain Science Institute, Saitama, 351-0198 (Japan); Calcium Oscillation Project, International Cooperative Research Project and Solution-Oriented Research for Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama, 332-0012 (Japan)

    2015-10-23

    Inositol 1,4,5-trisphosphate receptor (IP{sub 3}R) is a key regulator of intracellular Ca{sup 2+} concentration that release Ca{sup 2+} from Ca{sup 2+} stores in response to various external stimuli. IP{sub 3}R also works as a signal hub which form a platform for interacting with various proteins involved in diverse cell signaling. Previously, we have identified an IP{sub 3}R homolog in the parasitic protist, Trypanosoma cruzi (TcIP{sub 3}R). Parasites expressing reduced or increased levels of TcIP{sub 3}R displayed defects in growth, transformation, and infectivity. In the present study, we established parasitic strains expressing a dominant negative form of TcIP{sub 3}R, named DN-TcIP{sub 3}R, to further investigate the physiological role(s) of TcIP{sub 3}R. We found that the growth of epimastigotes expressing DN-TcIP{sub 3}R was significantly slower than that of parasites with TcIP{sub 3}R expression levels that were approximately 65% of wild-type levels. The expression of DN-TcIP{sub 3}R in epimastigotes induced metacyclogenesis even in the normal growth medium. Furthermore, these epimastigotes showed the presence of dense mitochondria under a transmission electron microscope. Our findings confirm that TcIP{sub 3}R is crucial for epimastigote growth, as previously reported. They also suggest that a strong inhibition of the IP{sub 3}R-mediated signaling induces metacyclogenesis and that mitochondrial integrity is closely associated with this signaling. - Highlights: • We established T. cruzi strains expressing a dominant negative form of the TcIP{sub 3}R. • DN-TcIP{sub 3}R expression inhibits epimastigote growth and induces metacyclogenesis. • Microscopic analysis indicated TcIP{sub 3}R role in maintaining mitochondrial integrity. • Growth, but not microbial density, was altered by mammalian IP{sub 3}R inhibitor (2-APB).

  7. D471G Mutation in LCMV-NP Affects Its Ability to Self-associate and Results in a Dominant Negative Effect in Viral RNA Synthesis

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    Luis Martínez-Sobrido

    2012-10-01

    Full Text Available Arenaviruses merit significant interest because several family members are etiological agents of severe hemorrhagic fevers, representing a major burden to public health. Currently, there are no FDA-licensed vaccines against arenaviruses and the only available antiviral therapy is limited to the use of ribavirin that is partially effective. Arenavirus nucleoprotein (NP is found associated with the genomic RNA forming the viral ribonucleoproteins (vRNPs that together with the polymerase (L direct viral replication and transcription. Virion formation requires the recruitment of vRNPs into budding sites, a process in which the arenavirus matrix-like protein (Z plays a major role. Therefore, proper NP-NP and NP-Z interactions are required for the generation of infectious progeny. In this work we demonstrate the role of the amino acid residue D471 in the self-association of lymphocytic choriomeningitis virus nucleoprotein (LCMV-NP. Amino acid substitutions at this position abrogate NP oligomerization, affecting its ability to mediate replication and transcription of a minigenome reporter plasmid. However, its ability to interact with the Z protein, counteract the cellular interferon response and bind to dsRNA analogs was retained. Additionally, we also document the dominant negative effect of D471G mutation on viral infection, suggesting that NP self-association is an excellent target for the development of new antivirals against arenaviruses.

  8. The thanatos mutation in Arabidopsis thaliana cellulose synthase 3 (AtCesA3) has a dominant-negative effect on cellulose synthesis and plant growth.

    Science.gov (United States)

    Daras, Gerasimos; Rigas, Stamatis; Penning, Bryan; Milioni, Dimitra; McCann, Maureen C; Carpita, Nicholas C; Fasseas, Constantinos; Hatzopoulos, Polydefkis

    2009-01-01

    Genetic functional analyses of mutants in plant genes encoding cellulose synthases (CesAs) have suggested that cellulose deposition requires the activity of multiple CesA proteins. Here, a genetic screen has led to the identification of thanatos (than), a semi-dominant mutant of Arabidopsis thaliana with impaired growth of seedlings. Homozygous seedlings of than germinate and grow but do not survive. In contrast to other CesA mutants, heterozygous plants are dwarfed and display a radially swollen root phenotype. Cellulose content is reduced by approximately one-fifth in heterozygous and by two-fifths in homozygous plants, showing gene-dosage dependence. Map-based cloning revealed an amino acid substitution (P578S) in the catalytic domain of the AtCesA3 gene, indicating a critical role for this residue in the structure and function of the cellulose synthase complex. Ab initio analysis of the AtCesA3 subdomain flanking the conserved proline residue predicted that the amino acid substitution to serine alters protein secondary structure in the catalytic domain. Gene dosage-dependent expression of the AtCesA3 mutant gene in wild-type A. thaliana plants resulted in a than dominant-negative phenotype. We propose that the incorporation of a mis-folded CesA3 subunit into the cellulose synthase complex may stall or prevent the formation of functional rosette complexes.

  9. Dominant negative RPW8.2 fusion proteins reveal the importance of haustorium-oriented protein trafficking for resistance against powdery mildew in Arabidopsis.

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    Zhang, Qiong; Berkey, Robert; Pan, Zhiyong; Wang, Wenming; Zhang, Yi; Ma, Xianfeng; King, Harlan; Xiao, Shunyuan

    2015-01-01

    Powdery mildew fungi form feeding structures called haustoria inside epidermal cells of host plants to extract photosynthates for their epiphytic growth and reproduction. The haustorium is encased by an interfacial membrane termed the extrahaustorial membrane (EHM). The atypical resistance protein RPW8.2 from Arabidopsis is specifically targeted to the EHM where RPW8.2 activates haustorium-targeted (thus broad-spectrum) resistance against powdery mildew fungi. EHM-specific localization of RPW8.2 suggests the existence of an EHM-oriented protein/membrane trafficking pathway during EHM biogenesis. However, the importance of this specific trafficking pathway for host defense has not been evaluated via a genetic approach without affecting other trafficking pathways. Here, we report that expression of EHM-oriented, nonfunctional RPW8.2 chimeric proteins exerts dominant negative effect over functional RPW8.2 and potentially over other EHM-localized defense proteins, thereby compromising both RPW8.2-mediated and basal resistance to powdery mildew. Thus, our results highlight the importance of the EHM-oriented protein/membrane trafficking pathway for host resistance against haustorium-forming pathogens such as powdery mildew fungi.

  10. Polyethyleneimine mediated DNA transfection in schistosome parasites and regulation of the WNT signaling pathway by a dominant-negative SmMef2.

    Directory of Open Access Journals (Sweden)

    Shuang Liang

    Full Text Available Schistosomiasis is a serious global problem and the second most devastating parasitic disease following malaria. Parasitic worms of the genus Schistosoma are the causative agents of schistosomiasis and infect more than 240 million people worldwide. The paucity of molecular tools to manipulate schistosome gene expression has made an understanding of genetic pathways in these parasites difficult, increasing the challenge of identifying new potential drugs for treatment. Here, we describe the use of a formulation of polyethyleneimine (PEI as an alternative to electroporation for the efficacious transfection of genetic material into schistosome parasites. We show efficient expression of genes from a heterologous CMV promoter and from the schistosome Sm23 promoter. Using the schistosome myocyte enhancer factor 2 (SmMef2, a transcriptional activator critical for myogenesis and other developmental pathways, we describe the development of a dominant-negative form of the schistosome Mef2. Using this mutant, we provide evidence that SmMef2 may regulate genes in the WNT pathway. We also show that SmMef2 regulates its own expression levels. These data demonstrate the use of PEI to facilitate effective transfection of nucleic acids into schistosomes, aiding in the study of schistosome gene expression and regulation, and development of genetic tools for the characterization of molecular pathways in these parasites.

  11. Transgenic Wuzhishan minipigs designed to express a dominant-negative porcine growth hormone receptor display small stature and a perturbed insulin/IGF-1 pathway.

    Science.gov (United States)

    Li, Feida; Li, Yong; Liu, Huan; Zhang, Xingju; Liu, Chuxin; Tian, Kai; Bolund, Lars; Dou, Hongwei; Yang, Wenxian; Yang, Huanming; Staunstrup, Nicklas Heine; Du, Yutao

    2015-12-01

    Growth hormone (GH) is an anabolic mitogen with widespread influence on cellular growth and differentiation as well as on glucose and lipid metabolism. GH binding to the growth hormone receptor (GHR) on hepatocytes prompts expression of insulin growth factor I (IGF-1) involved in nutritionally induced compensatory hyperplasia of pancreatic β-cell islets and insulin release. A prolonged hyperactivity of the IGF-1/insulin axis in the face of insulinotropic nutrition, on the other hand, can lead to collapse of the pancreatic islets and glucose intolerance. Individuals with Laron syndrome carry mutations in the GHR gene resulting in severe congenital IGF-1 deficiency and elevated GH serum levels leading to short stature as well as perturbed lipid and glucose metabolism. However, these individuals enjoy a reduced prevalence of acne, cancer and possibly diabetes. Minipigs have become important biomedical models for human conditions due to similarities in organ anatomy, physiology, and metabolism relative to humans. The purpose of this study was to generate transgenic Wuzhishan minipigs by handmade cloning with impaired systemic GHR activity and assess their growth profile and glucose metabolism. Transgenic minipigs featuring overexpression of a dominant-negative porcine GHR (GHR(dm)) presented postnatal growth retardation and proportionate dwarfism. Molecular changes included elevated GH serum levels and mild hyperglycemia. We believe that this model may prove valuable in the study of GH functions in relation to cancer, diabetes and longevity.

  12. Lymphoma-like T cell infiltration in liver is associated with increased copy number of dominant negative form of TGFβ receptor II.

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    Weici Zhang

    Full Text Available Hepatosplenic T cell lymphoma (HSTCL is a distinct and lethal subtype of peripheral T cell lymphoma with an aggressive course and poor outcome despite multiagent chemotherapy. Contradictory literature, an unknown etiology, and poor response to treatment highlight the need to define the malignant process and identify molecular targets with potential for successful therapeutic interventions. Herein, we report that mice homozygously expressing a dominant negative TGFβRII (dnTGFβRII under the control of the CD4 promoter spontaneously develop lymphoma-like T cell infiltration involving both spleen and liver. Splenomegaly, hepatomegaly and liver dysfunction were observed in homozygous dnTGFβRII mice between 10 weeks and 10 months of age associated with a predominant infiltration of CD4(-CD8(-TCRβ(+NK1.1(+ or CD8(+TCRβ(+NK1.1(- T cell subsets. Notch 1 and c-Myc expression at the mRNA levels were significantly increased and positively correlated with the cell number of lymphoid infiltrates in the liver of dnTGFβRII homozygous compared to hemizygous mice. Further, 2×10(4 isolated lymphoma-like cells transplant disease by adoptive cell transfers. Collectively, our data demonstrate that increased copy number of dnTGFβRII is critical for development of lymphoma-like T cell infiltration.

  13. Dominant negative mutant of Plasmodium Rad51 causes reduced parasite burden in host by abrogating DNA double-strand break repair.

    Science.gov (United States)

    Roy, Nabamita; Bhattacharyya, Sunanda; Chakrabarty, Swati; Laskar, Shyamasree; Babu, Somepalli Mastan; Bhattacharyya, Mrinal Kanti

    2014-10-01

    Malaria parasites survive through repairing a plethora of DNA double-stranded breaks (DSBs) experienced during their asexual growth. In Plasmodium Rad51 mediated homologous recombination (HR) mechanism and homology-independent alternative end-joining mechanism have been identified. Here we address whether loss of HR activity can be compensated by other DSB repair mechanisms. Creating a transgenic Plasmodium line defective in HR function, we demonstrate that HR is the most important DSB repair pathway in malarial parasite. Using mouse malaria model we have characterized the dominant negative effect of PfRad51(K143R) mutant on Plasmodium DSB repair and host-parasite interaction. Our work illustrates that Plasmodium berghei harbouring the mutant protein (PfRad51(K143R)) failed to repair DSBs as evidenced by hypersensitivity to DNA-damaging agent. Mice infected with mutant parasites lived significantly longer with markedly reduced parasite burden. To better understand the effect of mutant PfRad51(K143R) on HR, we used yeast as a surrogate model and established that the presence of PfRad51(K143R) completely inhibited DNA repair, gene conversion and gene targeting. Biochemical experiment confirmed that very low level of mutant protein was sufficient for complete disruption of wild-type PfRad51 activity. Hence our work provides evidence that HR pathway of Plasmodium could be efficiently targeted to curb malaria.

  14. A dominant negative form of inositol 1,4,5-trisphosphate receptor induces metacyclogenesis and increases mitochondrial density in Trypanosoma cruzi.

    Science.gov (United States)

    Hashimoto, Muneaki; Nara, Takeshi; Enomoto, Masahiro; Kurebayashi, Nagomi; Yoshida, Mitsutaka; Sakurai, Takashi; Mita, Toshihiro; Mikoshiba, Katsuhiko

    2015-10-23

    Inositol 1,4,5-trisphosphate receptor (IP3R) is a key regulator of intracellular Ca(2+) concentration that release Ca(2+) from Ca(2+) stores in response to various external stimuli. IP3R also works as a signal hub which form a platform for interacting with various proteins involved in diverse cell signaling. Previously, we have identified an IP3R homolog in the parasitic protist, Trypanosoma cruzi (TcIP3R). Parasites expressing reduced or increased levels of TcIP3R displayed defects in growth, transformation, and infectivity. In the present study, we established parasitic strains expressing a dominant negative form of TcIP3R, named DN-TcIP3R, to further investigate the physiological role(s) of TcIP3R. We found that the growth of epimastigotes expressing DN-TcIP3R was significantly slower than that of parasites with TcIP3R expression levels that were approximately 65% of wild-type levels. The expression of DN-TcIP3R in epimastigotes induced metacyclogenesis even in the normal growth medium. Furthermore, these epimastigotes showed the presence of dense mitochondria under a transmission electron microscope. Our findings confirm that TcIP3R is crucial for epimastigote growth, as previously reported. They also suggest that a strong inhibition of the IP3R-mediated signaling induces metacyclogenesis and that mitochondrial integrity is closely associated with this signaling.

  15. Immunization with a dominant-negative recombinant Herpes Simplex Virus (HSV type 1 protects against HSV-2 genital disease in guinea pigs

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    Brans Richard

    2010-06-01

    Full Text Available Abstract Background CJ9-gD is a novel dominant-negative recombinant herpes simplex virus type 1 (HSV-1 that is completely replication-defective, cannot establish detectable latent infection in vivo, and expresses high levels of the major HSV-1 antigen glycoprotein D immediately following infection. In the present study, CJ9-gD was evaluated as a vaccine against HSV-2 genital infection in guinea pigs. Results Animals immunized with CJ9-gD developed at least 700-fold higher titers of HSV-2-specific neutralization antibodies than mock-immunized controls. After challenge with wild-type HSV-2, all 10 control guinea pigs developed multiple genital lesions with an average of 21 lesions per animal. In contrast, only 2 minor lesions were found in 2 of 8 CJ9-gD-immunized animals, representing a 40-fold reduction on the incidence of primary genital lesions in immunized animals (p Conclusions Collectively, we demonstrate that vaccination with the HSV-1 recombinant CJ9-gD elicits strong and protective immune responses against primary and recurrent HSV-2 genital disease and significantly reduces the extent of latent infection.

  16. [Inhibition Function of Dominant-negative Mutant Gene Survivin-D53A to SPC-A1 Lung Adenocarcinoma Xenograft in Nude Mice Models].

    Science.gov (United States)

    Yu, Min; Peng, Xingchen; Lu, You; Huang, Meijuan

    2015-06-01

    Survivin-D53A (SVV-D53A) is a dominant-negative mutant survivin, which represents a potential promising target for cancer gene therapy. The present study was designed to determine whether SVV-D53A plasmid encapsuled by DOTAP: Chol liposome would have the anti-tumor activity against SPC-A1 lung adenocarcinoma, and to detect the possible mechanisms. In our experiment, SPC-A1 cells were transfected in vitro with SVV-D53A plasmid and examined for protein expression by Western blot, then flow cytometric analysis was used to detect apoptosis. SPC-A1 lung adenocarcinoma xenografts were established in vivo in the nude mice, which received the i. v. administrations of SVV-D53A plasmid/liposome complexes. After mice were sacrificed, the paraffin-embedded tumor tissue sections were used for proliferating cell nuclear antigen (PCNA) expression and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Compared with the control group, the mice treated with SVV-D53A plasmid had an obviously reduced tumor volume, with high level of apoptosis and decreased cell proliferation in tumor tissue. The research results proved that the administration of SVV-D53A plasmid resulted in significant inhibition of SPC-A1 cells both in vitro and in vivo. The functional mechanism is that the anti-tumor response causes and induces tumor cell apoptosis.

  17. Dominant-negative mutation p.Arg324Thr in KCNA1 impairs Kv1.1 channel function in episodic ataxia.

    Science.gov (United States)

    Tristán-Clavijo, Enriqueta; Scholl, Francisco G; Macaya, Alfons; Iglesias, Gemma; Rojas, Ana M; Lucas, Miguel; Castellano, Antonio; Martinez-Mir, Amalia

    2016-11-01

    Episodic ataxia type 1 is a rare autosomal dominant neurological disorder caused by mutations in the KCNA1 gene that encodes the α subunit of voltage-gated potassium channel Kv1.1. The functional consequences of identified mutations on channel function do not fully correlate with the clinical phenotype of patients. A clinical and genetic study was performed in a family with 5 patients with episodic ataxia type 1, with concurrent epilepsy in 1 of them. Protein expression, modeling, and electrophysiological analyses were performed to study Kv1.1 function. Whole-genome linkage and candidate gene analyses revealed the novel heterozygous mutation p.Arg324Thr in the KCNA1 gene. The encoded mutant Kv1.1 channel displays reduced currents and altered activation and inactivation. Taken together, we provide genetic and functional evidence that mutation p.Arg324Thr in the KCNA1 gene is pathogenic and results in episodic ataxia type 1 through a dominant-negative effect. © 2016 International Parkinson and Movement Disorder Society. © 2016 International Parkinson and Movement Disorder Society.

  18. The effect of DN (dominant-negative) Ku70 and reoxygenation on hypoxia cell-kill: evidence of hypoxia-induced potentially lethal damage.

    Science.gov (United States)

    Urano, Muneyasu; Li, Gloria C; He, Fuqiu; Minami, Akiko; Burgman, Paul; Ling, C Clifton

    2012-07-01

    To study the effect of DN (dominant-negative) Ku70 and reoxygenation on the hypoxia-induced cell-kill. Cell lines were human colorectal carcinoma HCT8 and HT29 cells and their respective derivatives, v-HCT8 and v-HT29 infected with DNKu70-containing adenovirus. Cells were plated in glass tubes and made hypoxic by flushing N(2) gas containing 0, 0.1 or 0.5% O(2). Cell survival was determined by colony formation assay immediately after 0-96 h hypoxia. To reoxygenate medium were replaced fresh following 48 or 72 h in hypoxia and cells were incubated in aerobic environment for 2-24 h before survival assay. When incubated in hypoxia, cells lost reproductive capability ∼ exponentially as a function of time in hypoxia, and depending on the O(2) concentration. DNKu70 rendered cells more prone to hypoxia-induced cell-kill. Following reoxygenation cell survival increased rapidly but without detectable cell proliferation during first 24 hours. This evinced hypoxia-induced potentially lethal damage (PLD) that was repairable upon reoxygenation. DNKu70 did not significantly inhibit this repair. Hypoxia-induced cell lethality was facilitated by DNKu70, but substantially repaired upon reoxygenation. This may have negative impact on the effect of reoxygenation in cancer therapy.

  19. A dominant negative heterozygous G87R mutation in the zinc transporter, ZnT-2 (SLC30A2), results in transient neonatal zinc deficiency.

    Science.gov (United States)

    Lasry, Inbal; Seo, Young Ah; Ityel, Hadas; Shalva, Nechama; Pode-Shakked, Ben; Glaser, Fabian; Berman, Bluma; Berezovsky, Igor; Goncearenco, Alexander; Klar, Aharon; Levy, Jacob; Anikster, Yair; Kelleher, Shannon L; Assaraf, Yehuda G

    2012-08-24

    Zinc is an essential mineral, and infants are particularly vulnerable to zinc deficiency as they require large amounts of zinc for their normal growth and development. We have recently described the first loss-of-function mutation (H54R) in the zinc transporter ZnT-2 (SLC30A2) in mothers with infants harboring transient neonatal zinc deficiency (TNZD). Here we identified and characterized a novel heterozygous G87R ZnT-2 mutation in two unrelated Ashkenazi Jewish mothers with infants displaying TNZD. Transient transfection of G87R ZnT-2 resulted in endoplasmic reticulum-Golgi retention, whereas the WT transporter properly localized to intracellular secretory vesicles in HC11 and MCF-7 cells. Consequently, G87R ZnT-2 showed decreased stability compared with WT ZnT-2 as revealed by Western blot analysis. Three-dimensional homology modeling based on the crystal structure of YiiP, a close zinc transporter homologue from Escherichia coli, revealed that the basic arginine residue of the mutant G87R points toward the membrane lipid core, suggesting misfolding and possible loss-of-function. Indeed, functional assays including vesicular zinc accumulation, zinc secretion, and cytoplasmic zinc pool assessment revealed markedly impaired zinc transport in G87R ZnT-2 transfectants. Moreover, co-transfection experiments with both mutant and WT transporters revealed a dominant negative effect of G87R ZnT-2 over the WT ZnT-2; this was associated with mislocalization, decreased stability, and loss of zinc transport activity of the WT ZnT-2 due to homodimerization observed upon immunoprecipitation experiments. These findings establish that inactivating ZnT-2 mutations are an underlying basis of TNZD and provide the first evidence for the dominant inheritance of heterozygous ZnT-2 mutations via negative dominance due to homodimer formation.

  20. An engineered transforming growth factor β (TGF-β) monomer that functions as a dominant negative to block TGF-β signaling

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    Kim, Sun Kyung; Barron, Lindsey; Hinck, Cynthia S.; Petrunak, Elyse M.; Cano, Kristin E.; Thangirala, Avinash; Iskra, Brian; Brothers, Molly; Vonberg, Machell; Leal, Belinda; Richter, Blair; Kodali, Ravindra; Taylor, Alexander B.; Du, Shoucheng; Barnes, Christopher O.; Sulea, Traian; Calero, Guillermo; Hart, P. John; Hart, Matthew J.; Demeler, Borries; Hinck, Andrew P. (Texas-HSC); (NRCC); (Pitt)

    2017-02-22

    The transforming growth factor β isoforms, TGF-β1, -β2, and -β3, are small secreted homodimeric signaling proteins with essential roles in regulating the adaptive immune system and maintaining the extracellular matrix. However, dysregulation of the TGF-β pathway is responsible for promoting the progression of several human diseases, including cancer and fibrosis. Despite the known importance of TGF-βs in promoting disease progression, no inhibitors have been approved for use in humans. Herein, we describe an engineered TGF-β monomer, lacking the heel helix, a structural motif essential for binding the TGF-β type I receptor (TβRI) but dispensable for binding the other receptor required for TGF-β signaling, the TGF-β type II receptor (TβRII), as an alternative therapeutic modality for blocking TGF-β signaling in humans. As shown through binding studies and crystallography, the engineered monomer retained the same overall structure of native TGF-β monomers and bound TβRII in an identical manner. Cell-based luciferase assays showed that the engineered monomer functioned as a dominant negative to inhibit TGF-β signaling with a Ki of 20–70 nM. Investigation of the mechanism showed that the high affinity of the engineered monomer for TβRII, coupled with its reduced ability to non-covalently dimerize and its inability to bind and recruit TβRI, enabled it to bind endogenous TβRII but prevented it from binding and recruiting TβRI to form a signaling complex. Such engineered monomers provide a new avenue to probe and manipulate TGF-β signaling and may inform similar modifications of other TGF-β family members.

  1. Expression of dominant-negative thyroid hormone receptor alpha1 in Leydig and Sertoli cells demonstrates no additional defect compared with expression in Sertoli cells only.

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    Betty Fumel

    Full Text Available In the testis, thyroid hormone (T3 regulates the number of gametes produced through its action on Sertoli cell proliferation. However, the role of T3 in the regulation of steroidogenesis is still controversial.The TRαAMI knock-in allele allows the generation of transgenic mice expressing a dominant-negative TRα1 (thyroid receptor α1 isoform restricted to specific target cells after Cre-loxP recombination. Here, we introduced this mutant allele in both Sertoli and Leydig cells using a novel aromatase-iCre (ARO-iCre line that expresses Cre recombinase under control of the human Cyp19(IIa/aromatase promoter.We showed that loxP recombination induced by this ARO-iCre is restricted to male and female gonads, and is effective in Sertoli and Leydig cells, but not in germ cells. We compared this model with the previous introduction of TRαAMI specifically in Sertoli cells in order to investigate T3 regulation of steroidogenesis. We demonstrated that TRαAMI-ARO males exhibited increased testis weight, increased sperm reserve in adulthood correlated to an increased proliferative index at P3 in vivo, and a loss of T3-response in vitro. Nevertheless, TRαAMI-ARO males showed normal fertility. This phenotype is similar to TRαAMI-SC males. Importantly, plasma testosterone and luteinizing hormone levels, as well as mRNA levels of steroidogenesis enzymes StAR, Cyp11a1 and Cyp17a1 were not affected in TRαAMI-ARO.We concluded that the presence of a mutant TRαAMI allele in both Leydig and Sertoli cells does not accentuate the phenotype in comparison with its presence in Sertoli cells only. This suggests that direct T3 regulation of steroidogenesis through TRα1 is moderate in Leydig cells, and that Sertoli cells are the main target of T3 action in the testis.

  2. Alternative Splicing Generates a Diacylglycerol Kinase α Transcript That Acts as a Dominant-Negative Modulator of Superoxide Production in Localized Aggressive Periodontitis

    Science.gov (United States)

    Batista, Eraldo L.; Kantarci, Alpdogan I.; Hasturk, Hatice; Van Dyke, Thomas E.

    2015-01-01

    Background Diacylglycerol (DAG), levels of which are tightly regulated by diacylglycerol kinases (DGKs), is a lipid mediator linked to key biologic functions. Members of the DGK family undergo alternative splicing, generating the protein diversity necessary to control different intracellular DAG pools. DGKα function is altered in polymorphonuclear neutrophils (PMNs) of patients with localized aggressive periodontitis (LAgP), suggesting a genetic basis. Here, the authors assess DGKα spliced transcripts in human LAgP neutrophils. Methods In an expression library of a patient with LAgP, PMNs were screened for different DGKα transcripts. Real-time polymerase chain reaction and in vitro expression assays were performed to assess the fate of different transcripts on protein translocation and superoxide production in human leukemia cells (HL-60) and COS-7 cells. Results A DGKα transcript that lacks exon 10 (DGKαΔ10) and generates a premature stop codon and a truncated protein was identified as being upregulated in LAgP neutrophils. In vitro assays revealed that DGKαΔ10 translocation occurred even in the absence of important regulatory motifs. Transfection of HL-60 neutrophil-like cells with the DGKαΔ10 spliced variant induced an increase in the stimulated production of su-peroxide anion replicating the phenotype of LAgP PMNs. Conclusion DGKαΔ10 can act as a dominant-negative transcript that can modulate superoxide production and provides an example of genetic regulation of the inflammatory response that may be relevant to human inflammatory diseases such as LAgP. J Periodontol 2014;85:934-943. PMID:24171497

  3. Dominant-Negative Effects of Adult-Onset Huntingtin Mutations Alter the Division of Human Embryonic Stem Cells-Derived Neural Cells.

    Science.gov (United States)

    Lopes, Carla; Aubert, Sophie; Bourgois-Rocha, Fany; Barnat, Monia; Rego, Ana Cristina; Déglon, Nicole; Perrier, Anselme L; Humbert, Sandrine

    2016-01-01

    Mutations of the huntingtin protein (HTT) gene underlie both adult-onset and juvenile forms of Huntington's disease (HD). HTT modulates mitotic spindle orientation and cell fate in mouse cortical progenitors from the ventricular zone. Using human embryonic stem cells (hESC) characterized as carrying mutations associated with adult-onset disease during pre-implantation genetic diagnosis, we investigated the influence of human HTT and of an adult-onset HD mutation on mitotic spindle orientation in human neural stem cells (NSCs) derived from hESCs. The RNAi-mediated silencing of both HTT alleles in neural stem cells derived from hESCs disrupted spindle orientation and led to the mislocalization of dynein, the p150Glued subunit of dynactin and the large nuclear mitotic apparatus (NuMA) protein. We also investigated the effect of the adult-onset HD mutation on the role of HTT during spindle orientation in NSCs derived from HD-hESCs. By combining SNP-targeting allele-specific silencing and gain-of-function approaches, we showed that a 46-glutamine expansion in human HTT was sufficient for a dominant-negative effect on spindle orientation and changes in the distribution within the spindle pole and the cell cortex of dynein, p150Glued and NuMA in neural cells. Thus, neural derivatives of disease-specific human pluripotent stem cells constitute a relevant biological resource for exploring the impact of adult-onset HD mutations of the HTT gene on the division of neural progenitors, with potential applications in HD drug discovery targeting HTT-dynein-p150Glued complex interactions.

  4. A CaV2.1 N-terminal fragment relieves the dominant-negative inhibition by an Episodic ataxia 2 mutant.

    Science.gov (United States)

    Dahimene, Shehrazade; Page, Karen M; Nieto-Rostro, Manuela; Pratt, Wendy S; D'Arco, Marianna; Dolphin, Annette C

    2016-09-01

    Episodic ataxia 2 (EA2) is an autosomal dominant disorder caused by mutations in the gene CACNA1A that encodes the pore-forming CaV2.1 calcium channel subunit. The majority of EA2 mutations reported so far are nonsense or deletion/insertion mutations predicted to form truncated proteins. Heterologous expression of wild-type CaV2.1, together with truncated constructs that mimic EA2 mutants, significantly suppressed wild-type calcium channel function, indicating that the truncated protein produces a dominant-negative effect (Jouvenceau et al., 2001; Page et al., 2004). A similar finding has been shown for CaV2.2 (Raghib et al., 2001). We show here that a highly conserved sequence in the cytoplasmic N-terminus is involved in this process, for both CaV2.1 and CaV2.2 channels. Additionally, we were able to interfere with the suppressive effect of an EA2 construct by mutating key N-terminal residues within it. We postulate that the N-terminus of the truncated channel plays an essential part in its interaction with the full-length CaV2.1, which prevents the correct folding of the wild-type channel. In agreement with this, we were able to disrupt the interaction between EA2 and the full length channel by co-expressing a free N-terminal peptide. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

  5. Aged dominant negative p38α MAPK mice are resistant to age-dependent decline in adult-neurogenesis and context discrimination fear conditioning.

    Science.gov (United States)

    Cortez, IbDanelo; Bulavin, Dmitry V; Wu, Ping; McGrath, Erica L; Cunningham, Kathryn A; Wakamiya, Maki; Papaconstantinou, John; Dineley, Kelly T

    2017-03-30

    A major aspect of mammalian aging is the decline in functional competence of many self-renewing cell types, including adult-born neuronal precursors. Since age-related senescence of self-renewal occurs simultaneously with chronic up-regulation of the p38MAPKalpha (p38α) signaling pathway, we used the dominant negative mouse model for attenuated p38α activity (DN-p38α(AF/+)) in which Thr180 and Tyr182 are mutated (T→A/Y→F) to prevent phosphorylation activation (DN-p38α(AF/+)) and kinase activity. As a result, aged DN-p38α(AF/+) mice are resistant to age-dependent decline in proliferation and regeneration of several peripheral tissue progenitors when compared to wild-type littermates. Aging is the major risk factor for non-inherited forms of Alzheimer's disease (AD); environmental and genetic risk factors that accelerate the senescence phenotype are thought to contribute to an individual's relative risk. In the present study, we evaluated aged DN-p38α(AF/+) and wildtype littermates in a series of behavioral paradigms to test if p38α mutant mice exhibit altered baseline abnormalities in neurological reflexes, locomotion, anxiety-like behavior, and age-dependent cognitive decline. While aged DN-p38α(AF/+) and wildtype littermates appear equal in all tested baseline neurological and behavioral parameters, DN-p38α(AF/+) exhibit superior context discrimination fear conditioning. Context discrimination is a cognitive task that is supported by proliferation and differentiation of adult-born neurons in the dentate gyrus of the hippocampus. Consistent with enhanced context discrimination in aged DN-p38α(AF/+), we discovered enhanced production of adult-born neurons in the dentate gyrus of DN-p38α(AF/+) mice compared to wildtype littermates. Our findings support the notion that p38α inhibition has therapeutic utility in aging diseases that affect cognition, such as AD.

  6. Rough endoplasmic reticulum trafficking errors by different classes of mutant dentin sialophosphoprotein (DSPP) cause dominant negative effects in both dentinogenesis imperfecta and dentin dysplasia by entrapping normal DSPP.

    Science.gov (United States)

    von Marschall, Zofia; Mok, Seeun; Phillips, Matthew D; McKnight, Dianalee A; Fisher, Larry W

    2012-06-01

    Families with nonsyndromic dentinogenesis imperfecta (DGI) and the milder, dentin dysplasia (DD), have mutations in one allele of the dentin sialophosphoprotein (DSPP) gene. Because loss of a single Dspp allele in mice (and likely, humans) causes no dental phenotype, the mechanism(s) underling the dominant negative effects were investigated. DSPP mutations occur in three classes. (The first class, the mid-leader missense mutation, Y6D, was not investigated in this report.) All other 5′ mutations of DSPP result in changes/loss in the first three amino acids (isoleucine-proline-valine [IPV]) of mature DSPP or, for the A15V missense mutation, some retention of the hydrophobic leader sequence. All of this second class of mutations caused mutant DSPP to be retained in the rough endoplasmic reticulum (rER) of transfected HEK293 cells. Trafficking out of the rER by coexpressed normal DSPP was reduced in a dose-responsive manner, probably due to formation of Ca2+-dependent complexes with the retained mutant DSPP. IPV-like sequences begin many secreted Ca2+-binding proteins, and changing the third amino acid to the charged aspartate (D) in three other acidic proteins also caused increased rER accumulation. Both the leader-retaining A15V and the long string of hydrophobic amino acids resulting from all known frameshift mutations within the 3′-encoded Ca2+-binding repeat domain (third class of mutations) caused retention by association of the mutant proteins with rER membranes. More 5′ frameshift mutations result in longer mutant hydrophobic domains, but the milder phenotype, DD, probably due to lower effectiveness of the remaining, shorter Ca2+-binding domain in capturing normal DSPP protein within the rER. This study presents evidence of a shared underlying mechanism of capturing of normal DSPP by two different classes of DSPP mutations and offers an explanation for the mild (DD-II) versus severe (DGI-II and III) nonsyndromic dentin phenotypes. Evidence is also

  7. Dominant negative phenotype of Bacillus thuringiensis Cry1Ab, Cry11Aa and Cry4Ba mutants suggest hetero-oligomer formation among different Cry toxins.

    Directory of Open Access Journals (Sweden)

    Daniela Carmona

    Full Text Available BACKGROUND: Bacillus thuringiensis Cry toxins are used worldwide in the control of different insect pests important in agriculture or in human health. The Cry proteins are pore-forming toxins that affect the midgut cell of target insects. It was shown that non-toxic Cry1Ab helix α-4 mutants had a dominant negative (DN phenotype inhibiting the toxicity of wildtype Cry1Ab when used in equimolar or sub-stoichiometric ratios (1∶1, 0.5∶1, mutant∶wt indicating that oligomer formation is a key step in toxicity of Cry toxins. METHODOLOGY/PRINCIPAL FINDINGS: The DN Cry1Ab-D136N/T143D mutant that is able to block toxicity of Cry1Ab toxin, was used to analyze its capacity to block the activity against Manduca sexta larvae of other Cry1 toxins, such as Cry1Aa, Cry1Ac, Cry1Ca, Cry1Da, Cry1Ea and Cry1Fa. Cry1Ab-DN mutant inhibited toxicity of Cry1Aa, Cry1Ac and Cry1Fa. In addition, we isolated mutants in helix α-4 of Cry4Ba and Cry11Aa, and demonstrate that Cry4Ba-E159K and Cry11Aa-V142D are inactive and completely block the toxicity against Aedes aegypti of both wildtype toxins, when used at sub-stoichiometric ratios, confirming a DN phenotype. As controls we analyzed Cry1Ab-R99A or Cry11Aa-E97A mutants that are located in helix α-3 and are affected in toxin oligomerization. These mutants do not show a DN phenotype but were able to block toxicity when used in 10∶1 or 100∶1 ratios (mutant∶wt probably by competition of binding with toxin receptors. CONCLUSIONS/SIGNIFICANCE: We show that DN phenotype can be observed among different Cry toxins suggesting that may interact in vivo forming hetero-oligomers. The DN phenotype cannot be observed in mutants affected in oligomerization, suggesting that this step is important to inhibit toxicity of other toxins.

  8. Attenuation of the slow component of delayed rectification, action potential prolongation, and triggered activity in mice expressing a dominant-negative Kv2 alpha subunit.

    Science.gov (United States)

    Xu, H; Barry, D M; Li, H; Brunet, S; Guo, W; Nerbonne, J M

    1999-10-01

    An in vivo experimental strategy, involving cardiac-specific expression of a mutant Kv 2.1 subunit that functions as a dominant negative, was exploited in studies focused on exploring the role of members of the Kv2 subfamily of pore-forming (alpha) subunits in the generation of functional voltage-gated K(+) channels in the mammalian heart. A mutant Kv2.1 alpha subunit (Kv2.1N216) was designed to produce a truncated protein containing the intracellular N terminus, the S1 membrane-spanning domain, and a portion of the S1/S2 loop. The truncated Kv2.1N216 was epitope tagged at the C terminus with the 8-amino acid FLAG peptide to generate Kv2. 1N216FLAG. No ionic currents are detected on expression of Kv2. 1N216FLAG in HEK-293 cells, although coexpression of this construct with wild-type Kv2.1 markedly reduced the amplitudes of Kv2. 1-induced currents. Using the alpha-myosin heavy chain promoter to direct cardiac specific expression of the transgene, 2 lines of Kv2. 1N216FLAG-expressing transgenic mice were generated. Electrophysiological recordings from ventricular myocytes isolated from these animals revealed that I(K, slow) is selectively reduced. The attenuation of I(K, slow) is accompanied by marked action potential prolongation, and, occasionally, spontaneous triggered activity (apparently induced by early afterdepolarizations) is observed. The time constant of inactivation of I(K, slow) in Kv2. 1N216FLAG-expressing cells (mean+/-SEM=830+/-103 ms; n=17) is accelerated compared with the time constant of I(K, slow) inactivation (mean+/-SEM=1147+/-57 ms; n=25) in nontransgenic cells. In addition, unlike I(K, slow) in wild-type cells, the component of I(K, slow) remaining in the Kv2.1N216FLAG-expressing cells is insensitive to 25 mmol/L tetraethylammonium. Taken together, these observations suggest that there are 2 distinct components of I(K, slow) in mouse ventricular myocytes and that Kv2 alpha subunits underlie the more slowly inactivating, tetraethylammonium

  9. AAV-dominant negative tumor necrosis factor (DN-TNF) gene transfer to the striatum does not rescue medium spiny neurons in the YAC128 mouse model of Huntington's disease.

    Science.gov (United States)

    Alto, Laura Taylor; Chen, Xi; Ruhn, Kelly A; Treviño, Isaac; Tansey, Malú G

    2014-01-01

    CNS inflammation is a hallmark of neurodegenerative disease, and recent studies suggest that the inflammatory response may contribute to neuronal demise. In particular, increased tumor necrosis factor (TNF) signaling is implicated in the pathology of both Parkinson's disease (PD) and Alzheimer's disease (AD). We have previously shown that localized gene delivery of dominant negative TNF to the degenerating brain region can limit pathology in animal models of PD and AD. TNF is upregulated in Huntington's disease (HD), like in PD and AD, but it is unknown whether TNF signaling contributes to neuronal degeneration in HD. We used in vivo gene delivery to test whether selective reduction of soluble TNF signaling could attenuate medium spiny neuron (MSN) degeneration in the YAC128 transgenic (TG) mouse model of Huntington's disease (HD). AAV vectors encoding cDNA for dominant-negative tumor necrosis factor (DN-TNF) or GFP (control) were injected into the striatum of young adult wild type WT and YAC128 TG mice and achieved 30-50% target coverage. Expression of dominant negative TNF protein was confirmed immunohistologically and biochemically and was maintained as mice aged to one year, but declined significantly over time. However, the extent of striatal DN-TNF gene transfer achieved in our studies was not sufficient to achieve robust effects on neuroinflammation, rescue degenerating MSNs or improve motor function in treated mice. Our findings suggest that alternative drug delivery strategies should be explored to determine whether greater target coverage by DN-TNF protein might afford some level of neuroprotection against HD-like pathology and/or that soluble TNF signaling may not be the primary driver of striatal neuroinflammation and MSN loss in YAC128 TG mice.

  10. The direct effect of Focal Adhesion Kinase (FAK, dominant-negative FAK, FAK-CD and FAK siRNA on gene expression and human MCF-7 breast cancer cell tumorigenesis

    Directory of Open Access Journals (Sweden)

    Zhang Li

    2009-08-01

    Full Text Available Abstract Background Focal adhesion kinase (FAK is a non-receptor tyrosine kinase that plays an important role in survival signaling. FAK has been shown to be overexpressed in breast cancer tumors at early stages of tumorigenesis. Methods To study the direct effect of FAK on breast tumorigenesis, we developed Tet-ON (tetracycline-inducible system of MCF-7 breast cancer cells stably transfected with FAK or dominant-negative, C-terminal domain of FAK (FAK-CD, and also FAKsiRNA with silenced FAK MCF-7 stable cell line. Increased expression of FAK in isogenic Tet-inducible MCF-7 cells caused increased cell growth, adhesion and soft agar colony formation in vitro, while expression of dominant-negative FAK inhibitor caused inhibition of these cellular processes. To study the role of induced FAK and FAK-CD in vivo, we inoculated these Tet-inducible cells in nude mice to generate tumors in the presence or absence of doxycycline in the drinking water. FAKsiRNA-MCF-7 cells were also injected into nude mice to generate xenograft tumors. Results Induction of FAK resulted in significant increased tumorigenesis, while induced FAK-CD resulted in decreased tumorigenesis. Taq Man Low Density Array assay demonstrated specific induction of FAKmRNA in MCF-7-Tet-ON-FAK cells. DMP1, encoding cyclin D binding myb-like protein 1 was one of the genes specifically affected by Tet-inducible FAK or FAK-CD in breast xenograft tumors. In addition, silencing of FAK in MCF-7 cells with FAK siRNA caused increased cell rounding, decreased cell viability in vitro and inhibited tumorigenesis in vivo. Importantly, Affymetrix microarray gene profiling analysis using Human Genome U133A GeneChips revealed >4300 genes, known to be involved in apoptosis, cell cycle, and adhesion that were significantly down- or up-regulated (p Conclusion Thus, these data for the first time demonstrate the direct effect of FAK expression and function on MCF-7 breast cancer tumorigenesis in vivo and reveal

  11. The C-terminal Domain (CTD) of Human DNA Glycosylase NEIL1 Is Required for Forming BERosome Repair Complex with DNA Replication Proteins at the Replicating Genome: DOMINANT NEGATIVE FUNCTION OF THE CTD.

    Science.gov (United States)

    Hegde, Pavana M; Dutta, Arijit; Sengupta, Shiladitya; Mitra, Joy; Adhikari, Sanjay; Tomkinson, Alan E; Li, Guo-Min; Boldogh, Istvan; Hazra, Tapas K; Mitra, Sankar; Hegde, Muralidhar L

    2015-08-21

    The human DNA glycosylase NEIL1 was recently demonstrated to initiate prereplicative base excision repair (BER) of oxidized bases in the replicating genome, thus preventing mutagenic replication. A significant fraction of NEIL1 in cells is present in large cellular complexes containing DNA replication and other repair proteins, as shown by gel filtration. However, how the interaction of NEIL1 affects its recruitment to the replication site for prereplicative repair was not investigated. Here, we show that NEIL1 binarily interacts with the proliferating cell nuclear antigen clamp loader replication factor C, DNA polymerase δ, and DNA ligase I in the absence of DNA via its non-conserved C-terminal domain (CTD); replication factor C interaction results in ∼8-fold stimulation of NEIL1 activity. Disruption of NEIL1 interactions within the BERosome complex, as observed for a NEIL1 deletion mutant (N311) lacking the CTD, not only inhibits complete BER in vitro but also prevents its chromatin association and reduced recruitment at replication foci in S phase cells. This suggests that the interaction of NEIL1 with replication and other BER proteins is required for efficient repair of the replicating genome. Consistently, the CTD polypeptide acts as a dominant negative inhibitor during in vitro repair, and its ectopic expression sensitizes human cells to reactive oxygen species. We conclude that multiple interactions among BER proteins lead to large complexes, which are critical for efficient BER in mammalian cells, and the CTD interaction could be targeted for enhancing drug/radiation sensitivity of tumor cells.

  12. Establishment of the acute myeloid leukemia cell line Kasumi-6 from a patient with a dominant-negative mutation in the DNA-binding region of the C/EBPalpha gene.

    Science.gov (United States)

    Asou, Hiroya; Gombart, Adrian F; Takeuchi, Seisho; Tanaka, Hideo; Tanioka, Maki; Matsui, Hirotaka; Kimura, Akiro; Inaba, Toshiya; Koeffler, H Phillip

    2003-02-01

    A myeloid leukemia cell line designated Kasumi-6 was established from the bone marrow cells of an individual with acute myeloid leukemia, subtype M2. Both the original leukemic cells and the Kasumi-6 cell line harbor a hemizygous point mutation in the gene encoding the CCAAT/enhancer binding protein alpha (C/EBPalpha), a critical myeloid transcriptional factor. The C to G transition at nucleotide 1063 of the C/EBPalpha gene results in amino acid transition R305P in the fork or hinge region between the DNA-binding basic region and the leucine zipper dimerization domain of the C/EBPalpha protein. The Kasumi-6 cells expressed both the p42 and p30 isoforms of the C/EBPalpha protein endogenously, but electrophoretic mobility shift assays demonstrated an absence of C/EBPalpha binding to its respective site. Exogenous expression of the mutant form of C/EBPalpha demonstrated that it was unable to bind DNA and activate transcription from a G-CSF receptor-luciferase reporter construct. Furthermore, coexpression of the wild-type and mutant forms revealed that the mutant form repressed reporter gene activation by the wild type in a dose-responsive manner. This was concomitant with a dose-responsive decrease in wild-type protein binding to the G-CSF receptor C/EBP site. The data suggest that the R305P alteration confers a dominant-negative property on the mutant C/EBPalpha protein whereby the mutant polypeptide heterodimerizes with the wild-type polypeptide and prevents it from binding to DNA, thus blocking transcriptional activation. The Kasumi-6 cell line can serve as a model to study the cellular and molecular biology of the non-t(8;21) M2 type of myeloid leukemia and can elucidate the role of mutated C/EBPalpha in leukemogenesis. Copyright 2003 Wiley-Liss, Inc.

  13. Sequestration of muscarinic acetylcholine receptor m2 subtypes. Facilitation by G protein-coupled receptor kinase (GRK2) and attenuation by a dominant-negative mutant of GRK2.

    Science.gov (United States)

    Tsuga, H; Kameyama, K; Haga, T; Kurose, H; Nagao, T

    1994-12-23

    Sequestration of m2 receptors (muscarinic acetylcholine receptor m2 subtypes), which was assessed as loss of N-[3H]methylscopolamine ([3H]NMS) binding activity from the cell surface, was examined in COS 7 and BHK-21 cells that had been transfected with expression vectors encoding the m2 receptor and, independently, vectors encoding a G protein-coupled receptor kinase (GRK2) (beta-adrenergic receptor kinase 1) or a GRK2 dominant-negative mutant (DN-GRK2). The sequestration of m2 receptors became apparent when the cells were treated with 10(-5) M or higher concentrations of carbamylcholine. In this case, approximately 40% or 20-25% of the [3H]NMS binding sites on COS 7 or BHK-21 cells, respectively, were sequestered with a half-life of 15-25 min. In cells in which GRK2 was also expressed, the sequestration became apparent in the presence of 10(-7) M carbamylcholine. Approximately 40% of the [3H]NMS binding sites on both COS 7 and BHK-21 cells were sequestered in the presence of 10(-6) M or higher concentrations of carbamylcholine. When DN-GRK2 was expressed in COS 7 cells, the proportion of [3H]NMS binding sites sequestered in the presence of 10(-5) M or higher concentrations of carbamylcholine was reduced to 20-30%. These results indicate that the phosphorylation of m2 receptors by GRK2 facilitates their sequestration. These results are in contrast with the absence of a correlation between sequestration and the phosphorylation of beta-adrenergic receptors by the GRK2 and suggests that the consequences of phosphorylation by GRK2 are different for different receptors.

  14. Extended Emotions

    DEFF Research Database (Denmark)

    Krueger, Joel; Szanto, Thomas

    2016-01-01

    Until recently, philosophers and psychologists conceived of emotions as brain- and body-bound affairs. But researchers have started to challenge this internalist and individualist orthodoxy. A rapidly growing body of work suggests that some emotions incorporate external resources and thus extend...... beyond the neurophysiological confines of organisms; some even argue that emotions can be socially extended and shared by multiple agents. Call this the extended emotions thesis (ExE). In this article, we consider different ways of understanding ExE in philosophy, psychology, and the cognitive sciences....... First, we outline the background of the debate and discuss different argumentative strategies for ExE. In particular, we distinguish ExE from cognate but more moderate claims about the embodied and situated nature of cognition and emotion (Section 1). We then dwell upon two dimensions of ExE: emotions...

  15. Extended thermodynamics

    CERN Document Server

    Müller, Ingo

    1993-01-01

    Physicists firmly believe that the differential equations of nature should be hyperbolic so as to exclude action at a distance; yet the equations of irreversible thermodynamics - those of Navier-Stokes and Fourier - are parabolic. This incompatibility between the expectation of physicists and the classical laws of thermodynamics has prompted the formulation of extended thermodynamics. After describing the motifs and early evolution of this new branch of irreversible thermodynamics, the authors apply the theory to mon-atomic gases, mixtures of gases, relativistic gases, and "gases" of phonons and photons. The discussion brings into perspective the various phenomena called second sound, such as heat propagation, propagation of shear stress and concentration, and the second sound in liquid helium. The formal mathematical structure of extended thermodynamics is exposed and the theory is shown to be fully compatible with the kinetic theory of gases. The study closes with the testing of extended thermodynamics thro...

  16. Extending Puppet

    CERN Document Server

    Franceschi, Alessandro

    2014-01-01

    This book is a clear, detailed and practical guide to learn about designing and deploying you puppet architecture, with informative examples to highlight and explain concepts in a focused manner. This book is designed for users who already have good experience with Puppet, and will surprise experienced users with innovative topics that explore how to design, implement, adapt, and deploy a Puppet architecture. The key to extending Puppet is the development of types and providers, for which you must be familiar with Ruby.

  17. Extending Experiences

    DEFF Research Database (Denmark)

    A computer game's player is experiencing not only the game as a designer-made artefact, but also a multitude of social and cultural practices and contexts of both computer game play and everyday life. As a truly multidisciplinary anthology, Extending Experiences sheds new light on the mesh...... of possibilities and influences the player engages with. Part one, Experiential Structures of Play, considers some of the key concepts commonly used to address the experience of a computer game player. The second part, Bordering Play, discusses conceptual and practical overlaps of games and everyday life...

  18. Consciousness extended

    DEFF Research Database (Denmark)

    Carrara-Augustenborg, Claudia

    2012-01-01

    There is no consensus yet regarding a conceptualization of consciousness able to accommodate all the features of such complex phenomenon. Different theoretical and empirical models lend strength to both the occurrence of a non-accessible informational broadcast, and to the mobilization of specific...... brain areas responsible for the emergence of the individual´s explicit and variable access to given segments of such broadcast. Rather than advocating one model over others, this chapter proposes to broaden the conceptualization of consciousness by letting it embrace both mechanisms. Within...... such extended framework, I propose conceptual and functional distinctions between consciousness (global broadcast of information), awareness (individual´s ability to access the content of such broadcast) and unconsciousness (focally isolated neural activations). My hypothesis is that a demarcation in terms...

  19. Extended Chameleons

    CERN Document Server

    Brax, Philippe

    2015-01-01

    We extend the chameleon models by considering Scalar-Fluid theories where the coupling between matter and the scalar field can be represented by a quadratic effective potential with density-dependent minimum and mass. In this context, we study the effects of the scalar field on Solar System tests of gravity and show that models passing these stringent constraints can still induce large modifications of Newton's law on galactic scales. On these scales we analyse models which could lead to a percent deviation of Newton's law outside the virial radius. We then model the dark matter halo as a Navarro-Frenk-White profile and explicitly find that the fifth force can give large contributions around the galactic core in a particular model where the scalar field mass is constant and the minimum of its potential varies linearly with the matter density. At cosmological distances, we find that this model does not alter the growth of large scale structures and therefore would be best tested on galactic scales, where inter...

  20. On generalized extending modules

    Institute of Scientific and Technical Information of China (English)

    ZENG Qing-yi

    2007-01-01

    A module M is called generalized extending if for any submodule N of M, there is a direct summand K of M such that N≤K and K/N is singular. Any extending module and any singular module are generalized extending. Any homomorphic image of a generalized extending module is generalized extending. Any direct sum of a singular (uniform) module and a semi-simple module is generalized extending. A ring R is a right Co-H-ring ifand only ifall right R modules are generalized extending modules.

  1. The C-terminal domain of Nrf1 negatively regulates the full-length CNC-bZIP factor and its shorter isoform LCR-F1/Nrf1β; both are also inhibited by the small dominant-negative Nrf1γ/δ isoforms that down-regulate ARE-battery gene expression.

    Science.gov (United States)

    Zhang, Yiguo; Qiu, Lu; Li, Shaojun; Xiang, Yuancai; Chen, Jiayu; Ren, Yonggang

    2014-01-01

    The C-terminal domain (CTD, aa 686-741) of nuclear factor-erythroid 2 p45-related factor 1 (Nrf1) shares 53% amino acid sequence identity with the equivalent Neh3 domain of Nrf2, a homologous transcription factor. The Neh3 positively regulates Nrf2, but whether the Neh3-like (Neh3L) CTD of Nrf1 has a similar role in regulating Nrf1-target gene expression is unknown. Herein, we report that CTD negatively regulates the full-length Nrf1 (i.e. 120-kDa glycoprotein and 95-kDa deglycoprotein) and its shorter isoform LCR-F1/Nrf1β (55-kDa). Attachment of its CTD-adjoining 112-aa to the C-terminus of Nrf2 yields the chimaeric Nrf2-C112Nrf1 factor with a markedly decreased activity. Live-cell imaging of GFP-CTD reveals that the extra-nuclear portion of the fusion protein is allowed to associate with the endoplasmic reticulum (ER) membrane through the amphipathic Neh3L region of Nrf1 and its basic c-tail. Thus removal of either the entire CTD or the essential Neh3L portion within CTD from Nrf1, LCR-F1/Nrf1β and Nrf2-C112Nrf1, results in an increase in their transcriptional ability to regulate antioxidant response element (ARE)-driven reporter genes. Further examinations unravel that two smaller isoforms, 36-kDa Nrf1γ and 25-kDa Nrf1δ, act as dominant-negative inhibitors to compete against Nrf1, LCR-F1/Nrf1β and Nrf2. Relative to Nrf1, LCR-F1/Nrf1β is a weak activator, that is positively regulated by its Asn/Ser/Thr-rich (NST) domain and acidic domain 2 (AD2). Like AD1 of Nrf1, both AD2 and NST domain of LCR-F1/Nrf1β fused within two different chimaeric contexts to yield Gal4D:Nrf1β607 and Nrf1β:C270Nrf2, positively regulate their transactivation activity of cognate Gal4- and Nrf2-target reporter genes. More importantly, differential expression of endogenous ARE-battery genes is attributable to up-regulation by Nrf1 and LCR-F1/Nrf1β and down-regulation by Nrf1γ and Nrf1δ.

  2. An integrin binding-defective mutant of insulin-like growth factor-1 (R36E/R37E IGF1) acts as a dominant-negative antagonist of the IGF1 receptor (IGF1R) and suppresses tumorigenesis but still binds to IGF1R.

    Science.gov (United States)

    Fujita, Masaaki; Ieguchi, Katsuaki; Cedano-Prieto, Dora M; Fong, Andrew; Wilkerson, Charles; Chen, Jane Q; Wu, Mac; Lo, Su-Hao; Cheung, Anthony T W; Wilson, Machelle D; Cardiff, Robert D; Borowsky, Alexander D; Takada, Yoko K; Takada, Yoshikazu

    2013-07-05

    Insulin-like growth factor-1 (IGF1) is a major therapeutic target for cancer. We recently reported that IGF1 directly binds to integrins (αvβ3 and α6β4) and induces ternary complex formation (integrin-IGF1-IGF1 receptor (IGF1R)) and that the integrin binding-defective mutant of IGF1 (R36E/R37E) is defective in signaling and ternary complex formation. These findings predict that R36E/R37E competes with WT IGF1 for binding to IGF1R and inhibits IGF signaling. Here, we described that excess R36E/R37E suppressed cell viability increased by WT IGF1 in vitro in non-transformed cells. We studied the effect of R36E/R37E on viability and tumorigenesis in cancer cell lines. We did not detect an effect of WT IGF1 or R36E/R37E in cancer cells under anchorage-dependent conditions. However, under anchorage-independent conditions, WT IGF1 enhanced cell viability and induced signals, whereas R36E/R37E did not. Notably, excess R36E/R37E suppressed cell viability and signaling induced by WT IGF1 under anchorage-independent conditions. Using cancer cells stably expressing WT IGF1 or R36E/R37E, we determined that R36E/R37E suppressed tumorigenesis in vivo, whereas WT IGF1 markedly enhanced it. R36E/R37E suppressed the binding of WT IGF1 to the cell surface and the subsequent ternary complex formation induced by WT IGF1. R36E/R37E suppressed activation of IGF1R by insulin. WT IGF1, but not R36E/R37E, induced ternary complex formation with the IGF1R/insulin receptor hybrid. These findings suggest that 1) IGF1 induces signals under anchorage-independent conditions and that 2) R36E/R37E acts as a dominant-negative inhibitor of IGF1R (IGF1 decoy). Our results are consistent with a model in which ternary complex formation is critical for IGF signaling.

  3. The C-terminal domain of Nrf1 negatively regulates the full-length CNC-bZIP factor and its shorter isoform LCR-F1/Nrf1β; both are also inhibited by the small dominant-negative Nrf1γ/δ isoforms that down-regulate ARE-battery gene expression.

    Directory of Open Access Journals (Sweden)

    Yiguo Zhang

    Full Text Available The C-terminal domain (CTD, aa 686-741 of nuclear factor-erythroid 2 p45-related factor 1 (Nrf1 shares 53% amino acid sequence identity with the equivalent Neh3 domain of Nrf2, a homologous transcription factor. The Neh3 positively regulates Nrf2, but whether the Neh3-like (Neh3L CTD of Nrf1 has a similar role in regulating Nrf1-target gene expression is unknown. Herein, we report that CTD negatively regulates the full-length Nrf1 (i.e. 120-kDa glycoprotein and 95-kDa deglycoprotein and its shorter isoform LCR-F1/Nrf1β (55-kDa. Attachment of its CTD-adjoining 112-aa to the C-terminus of Nrf2 yields the chimaeric Nrf2-C112Nrf1 factor with a markedly decreased activity. Live-cell imaging of GFP-CTD reveals that the extra-nuclear portion of the fusion protein is allowed to associate with the endoplasmic reticulum (ER membrane through the amphipathic Neh3L region of Nrf1 and its basic c-tail. Thus removal of either the entire CTD or the essential Neh3L portion within CTD from Nrf1, LCR-F1/Nrf1β and Nrf2-C112Nrf1, results in an increase in their transcriptional ability to regulate antioxidant response element (ARE-driven reporter genes. Further examinations unravel that two smaller isoforms, 36-kDa Nrf1γ and 25-kDa Nrf1δ, act as dominant-negative inhibitors to compete against Nrf1, LCR-F1/Nrf1β and Nrf2. Relative to Nrf1, LCR-F1/Nrf1β is a weak activator, that is positively regulated by its Asn/Ser/Thr-rich (NST domain and acidic domain 2 (AD2. Like AD1 of Nrf1, both AD2 and NST domain of LCR-F1/Nrf1β fused within two different chimaeric contexts to yield Gal4D:Nrf1β607 and Nrf1β:C270Nrf2, positively regulate their transactivation activity of cognate Gal4- and Nrf2-target reporter genes. More importantly, differential expression of endogenous ARE-battery genes is attributable to up-regulation by Nrf1 and LCR-F1/Nrf1β and down-regulation by Nrf1γ and Nrf1δ.

  4. Extended Life Coolant Testing

    Science.gov (United States)

    2016-06-06

    ELC – Extended Life Coolant SCA – Supplemental Coolant Additive SOW – Scope of Work SwRI – Southwest Research Institute TARDEC – Tank Automotive...ethylene or propylene glycol and 35% extended life coolant #1 (ELC1) with a balance of water. At a higher ELC1 content of 45% or 50%, the mass loss...UNCLASSIFIED TABLE OF CONTENTS EXTENDED LIFE COOLANT TESTING INTERIM REPORT TFLRF No. 478 by Gregory A. T. Hansen Edwin A

  5. Extended icosahedral structures

    CERN Document Server

    Jaric, Marko V

    1989-01-01

    Extended Icosahedral Structures discusses the concepts about crystal structures with extended icosahedral symmetry. This book is organized into six chapters that focus on actual modeling of extended icosahedral crystal structures. This text first presents a tiling approach to the modeling of icosahedral quasiperiodic crystals. It then describes the models for icosahedral alloys based on random connections between icosahedral units, with particular emphasis on diffraction properties. Other chapters examine the glassy structures with only icosahedral orientational order and the extent of tra

  6. Quantum Extended Supersymmetries

    CERN Document Server

    Grigore, D R; Grigore, Dan Radu; Scharf, Gunter

    2003-01-01

    We analyse some quantum multiplets associated with extended supersymmetries. We study in detail the general form of the causal (anti)commutation relations. The condition of positivity of the scalar product imposes severe restrictions on the (quantum) model. It is problematic if one can find out quantum extensions of the standard model with extended supersymmetries.

  7. Extended Theories of Gravitation

    Directory of Open Access Journals (Sweden)

    Fatibene Lorenzo

    2013-09-01

    Full Text Available Extended theories of gravitation are naturally singled out by an analysis inspired by the Ehelers-Pirani-Schild framework. In this framework the structure of spacetime is described by a Weyl geometry which is enforced by dynamics. Standard General Relativity is just one possible theory within the class of extended theories of gravitation. Also all Palatini f(R theories are shown to be extended theories of gravitation. This more general setting allows a more general interpretation scheme and more general possible couplings between gravity and matter. The definitions and constructions of extended theories will be reviewed. A general interpretation scheme will be considered for extended theories and some examples will be considered.

  8. The dialogically extended mind

    DEFF Research Database (Denmark)

    Fusaroli, Riccardo; Gangopadhyay, Nivedita; Tylén, Kristian

    2014-01-01

    A growing conceptual and empirical literature is advancing the idea that language extends our cognitive skills. One of the most influential positions holds that language – qua material symbols – facilitates individual thought processes by virtue of its material properties. Extending upon this model......, we argue that language enhances our cognitive capabilities in a much more radical way: The skilful engagement of public material symbols facilitates evolutionarily unprecedented modes of collective perception, action and reasoning (interpersonal synergies) creating dialogically extended minds. We...... relate our approach to other ideas about collective minds and review a number of empirical studies to identify the mechanisms enabling the constitution of interpersonal cognitive systems....

  9. The Extended Enterprise concept

    DEFF Research Database (Denmark)

    Larsen, Lars Bjørn; Vesterager, Johan; Gobbi, Chiara

    1999-01-01

    This paper provides an overview of the work that has been done regarding the Extended Enterprise concept in the Common Concept team of Globeman 21 including references to results deliverables concerning the development of the Extended Enterprise concept. The first section presents the basic concept...... picture from Globeman21, which illustrates the Globeman21 way of realising the Extended Enterprise concept. The second section presents the Globeman21 EE concept in a life cycle perspective, which to a large extent is based on the thoughts and ideas behind GERAM (ISO/DIS 15704)....

  10. Rational extended thermodynamics

    CERN Document Server

    Müller, Ingo

    1998-01-01

    Ordinary thermodynamics provides reliable results when the thermodynamic fields are smooth, in the sense that there are no steep gradients and no rapid changes. In fluids and gases this is the domain of the equations of Navier-Stokes and Fourier. Extended thermodynamics becomes relevant for rapidly varying and strongly inhomogeneous processes. Thus the propagation of high­ frequency waves, and the shape of shock waves, and the regression of small-scale fluctuation are governed by extended thermodynamics. The field equations of ordinary thermodynamics are parabolic while extended thermodynamics is governed by hyperbolic systems. The main ingredients of extended thermodynamics are • field equations of balance type, • constitutive quantities depending on the present local state and • entropy as a concave function of the state variables. This set of assumptions leads to first order quasi-linear symmetric hyperbolic systems of field equations; it guarantees the well-posedness of initial value problems and f...

  11. Symmetric Extended Ockham Algebras

    Institute of Scientific and Technical Information of China (English)

    T.S. Blyth; Jie Fang

    2003-01-01

    The variety eO of extended Ockham algebras consists of those algealgebra with an additional endomorphism k such that the unary operations f and k commute. Here, we consider the cO-algebras which have a property of symmetry. We show that there are thirty two non-isomorphic subdirectly irreducible symmetric extended MS-algebras and give a complete description of them.2000 Mathematics Subject Classification: 06D15, 06D30

  12. Extended spider cognition.

    Science.gov (United States)

    Japyassú, Hilton F; Laland, Kevin N

    2017-05-01

    There is a tension between the conception of cognition as a central nervous system (CNS) process and a view of cognition as extending towards the body or the contiguous environment. The centralised conception requires large or complex nervous systems to cope with complex environments. Conversely, the extended conception involves the outsourcing of information processing to the body or environment, thus making fewer demands on the processing power of the CNS. The evolution of extended cognition should be particularly favoured among small, generalist predators such as spiders, and here, we review the literature to evaluate the fit of empirical data with these contrasting models of cognition. Spiders do not seem to be cognitively limited, displaying a large diversity of learning processes, from habituation to contextual learning, including a sense of numerosity. To tease apart the central from the extended cognition, we apply the mutual manipulability criterion, testing the existence of reciprocal causal links between the putative elements of the system. We conclude that the web threads and configurations are integral parts of the cognitive systems. The extension of cognition to the web helps to explain some puzzling features of spider behaviour and seems to promote evolvability within the group, enhancing innovation through cognitive connectivity to variable habitat features. Graded changes in relative brain size could also be explained by outsourcing information processing to environmental features. More generally, niche-constructed structures emerge as prime candidates for extending animal cognition, generating the selective pressures that help to shape the evolving cognitive system.

  13. An extended day program

    Directory of Open Access Journals (Sweden)

    Ševkušić-Mandić Slavica G.

    2002-01-01

    Full Text Available The paper presents the results of a pilot project evaluation, carried out as an action investigation whose aim was to provide a better quality extended day for primary school students. The project included the training of teachers involved in extended day program, designing of special activities performed by teachers with children once a week as well as changes and equipping of premises where children stay. The aims of the program were conception and performance of activities in a less formal way than during regular instructional days, linking of learning at school and acquired knowledge to everyday experiences, and work on contents contributing to the development of child's interests and creativity. The program was accomplished in a Belgrade primary school during the 2001/2002 academic year, comprising students of 1st and 2nd grades (N=77. The effects of the program were monitored throughout the academic year (observation and teachers' reports on accomplished workshops and at the end of the academic year (teachers and students' opinions of the program, academic achievement and creativity of students attending the extended day program compared with students not attending it. Findings about positive effects of the program on students' broadening of interests and willingness to express themselves creatively, indicate unequivocally that there is a need for developing special extended day programs. The extended day program is an opportunity for school to exert greater educational influence that has yet to be tapped.

  14. Extending Critical Performativity

    DEFF Research Database (Denmark)

    Spicer, André; Alvesson, Mats; Kärreman, Dan

    2016-01-01

    In this article we extend the debate about critical performativity. We begin by outlining the basic tenets of critical performativity and how this has been applied in the study of management and organization. We then address recent critiques of critical performance. We note these arguments suffer...... from an undue focus on intra-academic debates; engage in author-itarian theoretical policing; feign relevance through symbolic radicalism; and repackage common sense. We take these critiques as an opportunity to offer an extended model of critical performativity that involves focusing on issues...

  15. Parameterization of extended systems

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik

    2006-01-01

    The YJBK parameterization (of all stabilizing controllers) is extended to handle systems with additional sensors and/or actuators. It is shown that the closed loop transfer function is still an affine function in the YJBK parameters in the nominal case. Further, some closed-loop stability results...

  16. Extended Irreversible Thermodynamics

    CERN Document Server

    Jou, David

    2010-01-01

    This is the 4th edition of the highly acclaimed monograph on Extended Irreversible Thermodynamics, a theory that goes beyond the classical theory of irreversible processes. In contrast to the classical approach, the basic variables describing the system are complemented by non-equilibrium quantities. The claims made for extended thermodynamics are confirmed by the kinetic theory of gases and statistical mechanics. The book covers a wide spectrum of applications, and also contains a thorough discussion of the foundations and the scope of the current theories on non-equilibrium thermodynamics. For this new edition, the authors critically revised existing material while taking into account the most recent developments in fast moving fields such as heat transport in micro- and nanosystems or fast solidification fronts in materials sciences. Several fundamental chapters have been revisited emphasizing physics and applications over mathematical derivations. Also, fundamental questions on the definition of non-equil...

  17. Introduction to Extended Electrodynamics

    CERN Document Server

    Donev, S

    1997-01-01

    This paper summarizes the motivations and results obtained so far in the frame of a particular non-linearization of Classical Electrodynamics, which was called Extended Electrodynamics. The main purpose pursued with this non-linear extension of the classical Maxwell's equations is to have a reliable field-theoretical approach in describing (3+1) soliton-like electromagnetic formations, in particular, to build an extended and finite field model of free photons and photon complexes. The first chapter gives a corresponding analysis of Maxwell theory and introduces the new equations. The second chapter gives a full account of the results, including the photon-like solutions, in the vacuum case. A new concept, called scale factor, is defined and successfully used. Two ways for describing the intrinsic angular momentum are given. Interference of two photon-like solutions is also considered. The third chapter considers interaction with external fields (continuous media) on the base of establishing correspondence bet...

  18. Extended Theories of Gravitation

    Directory of Open Access Journals (Sweden)

    Fatibene Lorenzo

    2013-09-01

    Full Text Available Within the framework of extended theories of gravitation we shall discuss physical equivalences among different formalisms and classical tests. As suggested by the Ehlers-Pirani-Schild framework, the conformal invariance will be preserved and its effect on observational protocols discussed. Accordingly, we shall review standard tests showing how Palatini f(R-theories naturally passes solar system tests. Observation protocols will be discussed in this wider framework.

  19. Extended Ensemble Monte Carlo

    OpenAIRE

    Iba, Yukito

    2000-01-01

    ``Extended Ensemble Monte Carlo''is a generic term that indicates a set of algorithms which are now popular in a variety of fields in physics and statistical information processing. Exchange Monte Carlo (Metropolis-Coupled Chain, Parallel Tempering), Simulated Tempering (Expanded Ensemble Monte Carlo), and Multicanonical Monte Carlo (Adaptive Umbrella Sampling) are typical members of this family. Here we give a cross-disciplinary survey of these algorithms with special emphasis on the great f...

  20. Propelling Extended Objects

    Science.gov (United States)

    Humbert, Richard

    2010-01-01

    A force acting on just part of an extended object (either a solid or a volume of a liquid) can cause all of it to move. That motion is due to the transmission of the force through the object by its material. This paper discusses how the force is distributed to all of the object by a gradient of stress or pressure in it, which creates the local…

  1. The Fly Printer - Extended

    DEFF Research Database (Denmark)

    Beloff, Laura; Klaus, Malena

    2016-01-01

    Artist talk / Work-in-progress What is the purpose of a machine or an artifact, like the Fly Printer, that is dislocated, that produces images that have no meaning, no instrumentality, that depict nothing in the world? The biological and the cultural are reunited in this apparatus as a possibilit...... the results. The extended version of the Fly Printer containing the technological perception and DNNs is a collaboration between Laura Beloff and Malene Theres Klaus...

  2. extendFromReads

    Energy Technology Data Exchange (ETDEWEB)

    2013-10-03

    This package assists in genome assembly. extendFromReads takes as input a set of Illumina (eg, MiSeq) DNA sequencing reads, a query seed sequence and a direction to extend the seed. The algorithm collects all seed--]matching reads (flipping reverse--]orientation hits), trims off the seed and additional sequence in the other direction, sorts the remaining sequences alphabetically, and prints them aligned without gaps from the point of seed trimming. This produces a visual display distinguishing the flanks of multi-]copy seeds. A companion script hitMates.pl collects the mates of seed--]hi]ng reads, whose alignment reveals longer extensions from the seed. The collect/trim/sort strategy was made iterative and scaled up in the script denovo.pl, for de novo contig assembly. An index is pre--]built using indexReads.pl that for each unique 21--]mer found in all the reads, records its gfateh of extension (whether extendable, blocked by low coverage, or blocked by branching after a duplicated sequence) and other characteristics. Importantly, denovo.pl records all branchings that follow a branching contig endpoint, providing contig-]extension information

  3. Inequalities of extended beta and extended hypergeometric functions.

    Science.gov (United States)

    Mondal, Saiful R

    2017-01-01

    We study the log-convexity of the extended beta functions. As a consequence, we establish Turán-type inequalities. The monotonicity, log-convexity, log-concavity of extended hypergeometric functions are deduced by using the inequalities on extended beta functions. The particular cases of those results also give the Turán-type inequalities for extended confluent and extended Gaussian hypergeometric functions. Some reverses of Turán-type inequalities are also derived.

  4. Extended Testability Analysis Tool

    Science.gov (United States)

    Melcher, Kevin; Maul, William A.; Fulton, Christopher

    2012-01-01

    The Extended Testability Analysis (ETA) Tool is a software application that supports fault management (FM) by performing testability analyses on the fault propagation model of a given system. Fault management includes the prevention of faults through robust design margins and quality assurance methods, or the mitigation of system failures. Fault management requires an understanding of the system design and operation, potential failure mechanisms within the system, and the propagation of those potential failures through the system. The purpose of the ETA Tool software is to process the testability analysis results from a commercial software program called TEAMS Designer in order to provide a detailed set of diagnostic assessment reports. The ETA Tool is a command-line process with several user-selectable report output options. The ETA Tool also extends the COTS testability analysis and enables variation studies with sensor sensitivity impacts on system diagnostics and component isolation using a single testability output. The ETA Tool can also provide extended analyses from a single set of testability output files. The following analysis reports are available to the user: (1) the Detectability Report provides a breakdown of how each tested failure mode was detected, (2) the Test Utilization Report identifies all the failure modes that each test detects, (3) the Failure Mode Isolation Report demonstrates the system s ability to discriminate between failure modes, (4) the Component Isolation Report demonstrates the system s ability to discriminate between failure modes relative to the components containing the failure modes, (5) the Sensor Sensor Sensitivity Analysis Report shows the diagnostic impact due to loss of sensor information, and (6) the Effect Mapping Report identifies failure modes that result in specified system-level effects.

  5. Extended associative memories

    OpenAIRE

    J.H Sossa Azuela; R. Barrón Fernández

    2007-01-01

    The #945; #946; associative memories recently developed in Ref 10 have proven to be powerful tools for memorizing and recalling patterns when they appear distorted by noise. However they are only useful in the binary case. In this paper we show that it is possible to extend these memories now to the gray-level case. To get the desired extension, we take the original operators #945; and #946;, foundation of the #945; #946; memories, and propose a more general family of operators. We find t...

  6. Branes in extended spacetime

    CERN Document Server

    Sakatani, Yuho

    2016-01-01

    We propose a novel approach to the brane worldvolume theory based on the geometry of extended field theories; double field theory and exceptional field theory. We demonstrate the effectiveness of this approach by showing that one can reproduce the conventional bosonic string/membrane actions, and the M5-brane action in the weak field approximation. At a glance, the proposed 5-brane action without approximation looks different from the known M5-brane actions, but it is consistent with the known non-linear self-duality relation, and it may provide a new formulation of a single M5-brane action. Actions for exotic branes are also discussed.

  7. Extending the MINLO method

    CERN Document Server

    Frederix, Rikkert

    2015-01-01

    We consider improving POWHEG+MINLO simulations, so as to also render them NLO accurate in the description of observables receiving contributions from events with lower parton multiplicity than present in their underlying NLO calculation. On a conceptual level we follow the strategy of the so-called MINLO' programs. Whereas the existing MINLO' framework requires explicit analytic input from higher order resummation, here we derive an effective numerical approximation to these ingredients, by imposing unitarity. This offers a way of extending the MINLO' method to more complex processes, complementary to the known route which uses explicit computations of high-accuracy resummation inputs. Specifically, we have focused on Higgs-plus-two-jet production (HJJ) and related processes. We also consider how one can cover three units of multiplicity at NLO accuracy, i.e. we consider how the HJJ-MINLO simulation may yield NLO accuracy for inclusive H, HJ, and HJJ quantities. We perform a feasibility study assessing the po...

  8. Extending juvenility in grasses

    Energy Technology Data Exchange (ETDEWEB)

    Kaeppler, Shawn; de Leon Gatti, Natalia; Foerster, Jillian

    2017-04-11

    The present invention relates to compositions and methods for modulating the juvenile to adult developmental growth transition in plants, such as grasses (e.g. maize). In particular, the invention provides methods for enhancing agronomic properties in plants by modulating expression of GRMZM2G362718, GRMZM2G096016, or homologs thereof. Modulation of expression of one or more additional genes which affect juvenile to adult developmental growth transition such as Glossy15 or Cg1, in conjunction with such modulation of expression is also contemplated. Nucleic acid constructs for down-regulation of GRMZM2G362718 and/or GRMZM2G096016 are also contemplated, as are transgenic plants and products produced there from, that demonstrate altered, such as extended juvenile growth, and display associated phenotypes such as enhanced yield, improved digestibility, and increased disease resistance. Plants described herein may be used, for example, as improved forage or feed crops or in biofuel production.

  9. Extended Theories of Gravity

    CERN Document Server

    Capozziello, Salvatore

    2011-01-01

    Extended Theories of Gravity can be considered a new paradigm to cure shortcomings of General Relativity at infrared and ultraviolet scales. They are an approach that, by preserving the undoubtedly positive results of Einstein's Theory, is aimed to address conceptual and experimental problems recently emerged in Astrophysics, Cosmology and High Energy Physics. In particular, the goal is to encompass, in a self-consistent scheme, problems like Inflation, Dark Energy, Dark Matter, Large Scale Structure and, first of all, to give at least an effective description of Quantum Gravity. We review the basic principles that any gravitational theory has to follow. The geometrical interpretation is discussed in a broad perspective in order to highlight the basic assumptions of General Relativity and its possible extensions in the general framework of gauge theories. Principles of such modifications are presented, focusing on specific classes of theories like f (R)-gravity and scalar-tensor gravity in the metric and Pala...

  10. Extending over time

    DEFF Research Database (Denmark)

    Christensen, Tanya Karoli; Jensen, Torben Juel; Christensen, Marie Herget

    Studies of general extenders (GEs), such as Eng. and stuff like that, or something, typically find that it is a feature of youth speech, sometimes correlated with sex and class (e.g. Dubois 1992, Stubbe and Holmes 1995, Cheshire 2007, Tagliamonte and Denis 2010, Pichler and Levey 2011), but only...... that variants with sådan noget, though prevalent across the board, may be stigmatized, since they are produced mainly by young WC males, and exhibit an overall drop in frequency over time. In our paper, we will use GEs in Danish as a case study for evaluating prevailing assumptions about the relationship...... few have a design enabling them to distinguish unequivocally between age grading and communal change. In this paper, we present the results of a large-scale study of GEs in Danish, based on Copenhagen data from the LANCHART corpus, encompassing speech from three age cohorts, of which two have been...

  11. Extended Poisson Exponential Distribution

    Directory of Open Access Journals (Sweden)

    Anum Fatima

    2015-09-01

    Full Text Available A new mixture of Modified Exponential (ME and Poisson distribution has been introduced in this paper. Taking the Maximum of Modified Exponential random variable when the sample size follows a zero truncated Poisson distribution we have derived the new distribution, named as Extended Poisson Exponential distribution. This distribution possesses increasing and decreasing failure rates. The Poisson-Exponential, Modified Exponential and Exponential distributions are special cases of this distribution. We have also investigated some mathematical properties of the distribution along with Information entropies and Order statistics of the distribution. The estimation of parameters has been obtained using the Maximum Likelihood Estimation procedure. Finally we have illustrated a real data application of our distribution.

  12. Extended Ewald summation technique

    Science.gov (United States)

    Kylänpää, Ilkka; Räsänen, Esa

    2016-09-01

    We present a technique to improve the accuracy and to reduce the computational labor in the calculation of long-range interactions in systems with periodic boundary conditions. We extend the well-known Ewald method by using a linear combination of screening Gaussian charge distributions instead of only one. This enables us to find faster converging real-space and reciprocal space summations. The combined simplicity and efficiency of our method is demonstrated, and the scheme is readily applicable to large-scale periodic simulations, classical as well as quantum. Moreover, apart from the required a priori optimization the method is straightforward to include in most routines based on the Ewald method within, e.g., density-functional, molecular dynamics, and quantum Monte Carlo calculations.

  13. Stars with Extended Atmospheres

    Science.gov (United States)

    Sterken, C.

    2002-12-01

    This Workshop consisted of a full-day meeting of the Working Group "Sterren met Uitgebreide Atmosferen" (SUA, Working Group Stars with Extended Atmospheres), a discussion group founded in 1979 by Kees de Jager, Karel van der Hucht and Pik Sin The. This loose association of astronomers and astronomy students working in the Dutch-speaking part of the Low Countries (The Netherlands and Flanders) organised at regular intervals one-day meetings at the Universities of Utrecht, Leiden, Amsterdam and Brussels. These meetings consisted of the presentation of scientific results by junior as well as senior members of the group, and by discussions between the participants. As such, the SUA meetings became a forum for the exchange of ideas, and for asking questions and advice in an informal atmosphere. Kees de Jager has been chairman of the WG SUA from the beginning in 1979 till today, as the leading source of inspiration. At the occasion of Prof. Kees de Jager's 80th birthday, we decided to collect the presented talks in written form as a Festschrift in honour of this well-respected and much beloved scientist, teacher and friend. The first three papers deal with the personality of Kees de Jager, more specifically with his role as a supervisor and mentor of young researchers and as a catalyst in the research work of his colleagues. And also about his remarkable role in the establishment of astronomy education and research at the University of Brussels. The next presentation is a very detailed review of solar research, a field in which Cees was prominently active for many years. Then follow several papers dealing with stars about which Kees is a true expert: massive stars and extended atmospheres.

  14. Can Asymmetry of Solar Activity be Extended into Extended Cycle?

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    With the use of the Royal Greenwich Observatory data set of sunspot groups, an attempt is made to examine the north-south asymmetry of solar activity in the "extended" solar cycles. It is inferred that the asymmetry established for individual solar cycles does not extend to the "extended" cycles.

  15. Extended chameleon models

    Science.gov (United States)

    Brax, Philippe; Tamanini, Nicola

    2016-05-01

    We extend the chameleon models by considering scalar-fluid theories where the coupling between matter and the scalar field can be represented by a quadratic effective potential with density-dependent minimum and mass. In this context, we study the effects of the scalar field on Solar System tests of gravity and show that models passing these stringent constraints can still induce large modifications of Newton's law on galactic scales. On these scales we analyze models which could lead to a percent deviation of Newton's law outside the virial radius. We then model the dark matter halo as a Navarro-Frenk-White profile and explicitly find that the fifth force can give large contributions around the galactic core in a particular model where the scalar field mass is constant and the minimum of its potential varies linearly with the matter density. At cosmological distances, we find that this model does not alter the growth of large scale structures and therefore would be best tested on galactic scales, where interesting signatures might arise in the galaxy rotation curves.

  16. Chirally extended quantum chromodynamics

    CERN Document Server

    Brower, R C; Tan, C I; Richard C Brower; Yue Shen; Chung-I Tan

    1994-01-01

    We propose an extended Quantum Chromodynamics (XQCD) Lagrangian in which the fermions are coupled to elementary scalar %\\sigma and \\pi fields through a Yukawa coupling which preserves chiral invariance. Our principle motivation is to find a new lattice formulation for QCD which avoids the source of critical slowing down usually encountered as the bare quark mass is tuned to the chiral limit. The phase diagram and the weak coupling limit for XQCD are studied. They suggest a conjecture that the continuum limit of XQCD is the same as the continuum limit of conventional lattice formulation of QCD. As examples of such universality, we present the large N solutions of two prototype models for XQCD, in which the mass of the spurious pion and sigma resonance go to infinity with the cut-off. Even if the universality conjecture turns out to be false, we believe that XQCD will still be useful as a low energy effective action for QCD phenomenology on the lattice. Numerical simulations are recommended to further investiga...

  17. On the extended loop calculus

    CERN Document Server

    Griego, J R

    1995-01-01

    Some features of extended loops are considered. In particular, the behaviour under diffeomorphism transformations of the wavefunctions with support on the extended loop space are studied. The basis of a method to obtain analytical expressions of diffeomorphism invariants via extended loops are settled. Applications to knot theory and quantum gravity are considered.

  18. Extended Mixed Vector Equilibrium Problems

    Directory of Open Access Journals (Sweden)

    Mijanur Rahaman

    2014-01-01

    Full Text Available We study extended mixed vector equilibrium problems, namely, extended weak mixed vector equilibrium problem and extended strong mixed vector equilibrium problem in Hausdorff topological vector spaces. Using generalized KKM-Fan theorem (Ben-El-Mechaiekh et al.; 2005, some existence results for both problems are proved in noncompact domain.

  19. Suppressing autoimmunity in Arabidopsis thaliana with dominant negative immune receptors

    DEFF Research Database (Denmark)

    Greeff, Michael Christiaan

    2014-01-01

    R genes, as we have recently found that transgenics with similarly mutated rpm1-DN alleles lose resistance to Pseudomonas syringae expressing the AvrRpm1 effector. Accordingly, we have constructed a collection of 100 R-DN alleles and transformed them into other autoimmune mutants including camta3...

  20. Sonic Hedgehog Mutations Identified in Holoprosencephaly Patients Can Act in a Dominant Negative Manner

    OpenAIRE

    Singh, Samer; Tokhunts, Robert; Baubet, Valerie; Goetz, John A.; Huang, Zhen Jane; Schilling, Neal S.; Black, Kendall E.; MacKenzie, Todd A.; Dahmane, Nadia; Robbins, David J.

    2008-01-01

    Sonic Hedgehog (SHH) plays an important instructional role in vertebrate development, as exemplified by the numerous developmental disorders that occur when the SHH pathway is disrupted. Mutations in the SHH gene are the most common cause of sporadic and inherited Holoprosencephaly (HPE), a developmental disorder that is characterized by defective prosencephalon development. SHH HPE mutations provide a unique opportunity to better understand SHH biogenesis and signaling, and to decipher its r...

  1. MicroRNA-146a: A dominant, negative regulator of the innate immune response

    Directory of Open Access Journals (Sweden)

    Stephanie eBooth

    2014-11-01

    Full Text Available MicroRNAs (miRNAs are a class of small non-coding RNA molecules that can play critical roles as regulators of numerous pathways and biological processes including the immune response. Emerging as one of the most important miRNAs to orchestrate immune and inflammatory signaling, often through its recognized target genes, IRAK1 and TRAF6, is microRNA-146a (miR-146a. MiR-146a is one, of a small number of miRNAs, whose expression is strongly induced following challenge of cells with bacterial endotoxin, and prolonged expression has been linked to immune tolerance, implying that it acts as a fine tuning mechanism to prevent an overstimulation of the inflammatory response. In other cells, miR-146a has been shown to play a role in the control of the differentiation of megakaryocytic and monocytic lineages, adaptive immunity and cancer. In this review, we discuss the central role prescribed to miR-146a in innate immunity. We particularly focus on the role played by miR-146a in the regulation and signaling mediated by one of the main pattern recognition receptors, Toll/IL-1 receptors (TLRs. Additionally, we also discuss the role of miR-146a in several classes of autoimmune pathologies where this miRNA has been shown to be dysregulated, as well as its potential role in the pathobiology of neurodegenerative diseases.

  2. A recessive mutation in the APP gene with dominant-negative effect on amyloidogenesis.

    Science.gov (United States)

    Di Fede, Giuseppe; Catania, Marcella; Morbin, Michela; Rossi, Giacomina; Suardi, Silvia; Mazzoleni, Giulia; Merlin, Marco; Giovagnoli, Anna Rita; Prioni, Sara; Erbetta, Alessandra; Falcone, Chiara; Gobbi, Marco; Colombo, Laura; Bastone, Antonio; Beeg, Marten; Manzoni, Claudia; Francescucci, Bruna; Spagnoli, Alberto; Cantù, Laura; Del Favero, Elena; Levy, Efrat; Salmona, Mario; Tagliavini, Fabrizio

    2009-03-13

    beta-Amyloid precursor protein (APP) mutations cause familial Alzheimer's disease with nearly complete penetrance. We found an APP mutation [alanine-673-->valine-673 (A673V)] that causes disease only in the homozygous state, whereas heterozygous carriers were unaffected, consistent with a recessive Mendelian trait of inheritance. The A673V mutation affected APP processing, resulting in enhanced beta-amyloid (Abeta) production and formation of amyloid fibrils in vitro. Co-incubation of mutated and wild-type peptides conferred instability on Abeta aggregates and inhibited amyloidogenesis and neurotoxicity. The highly amyloidogenic effect of the A673V mutation in the homozygous state and its anti-amyloidogenic effect in the heterozygous state account for the autosomal recessive pattern of inheritance and have implications for genetic screening and the potential treatment of Alzheimer's disease.

  3. Truncated forms of BNIP3 act as dominant negatives inhibiting hypoxia-induced cell death

    Science.gov (United States)

    Bristow, Nicolle; Burton, Teralee R; Henson, Elizabeth S; Ong-Justiniano, Coleen; Brown, Michelle; Gibson, Spencer B

    2011-01-01

    BNIP3 (Bcl-2/adenovirus E1B Ninteen Kilodalton Interacting Protein) is a pro-cell death member of the Bcl-2 family of proteins. Its expression is induced by the transcription factor Hypoxia Inducible Factor-1 (HIF-1) under conditions of low oxygen (hypoxia) and is found over expressed in hypoxic regions of many tumors. When over expressed, BNIP3 induces cell death through induction of mitochondrial dysfunction that is dependant on the presence of BNIP3’s TM domain. Herein, we have determined that the SkOv3 ovarian cancer cell line expresses a truncated BNIP3 protein, which results in the elimination of the transmembrane domain. Truncation that eliminates all four domains of BNIP3 protein also inhibits hypoxia-induced cell death in SkOv3, HEK293, U251 and MCF-7 cells. Three different mutations in a BNIP3 expression vector that lead to a truncated BNIP3 protein, lacking TM domain only, or lacking CD, BH3, and TM domains resulted in inhibition of hypoxia-induced cell death when transfected into HEK293 cells. We found that truncated BNIP3 failed to associate with the mitochondria and the truncated BNIP3 lacking all four domains can bind to wild type BNIP3. Taken together, truncation of BNIP3 could be a novel mechanism for cancer cells to avoid hypoxia-induced cell death mediated by BNIP3 over expression. PMID:21138765

  4. Novel variants in GNAI3 associated with auriculocondylar syndrome strengthen a common dominant negative effect

    OpenAIRE

    2014-01-01

    Auriculocondylar syndrome is a rare craniofacial disorder comprising core features of micrognathia, condyle dysplasia and question mark ear. Causative variants have been identified in PLCB4, GNAI3 and EDN1, which are predicted to function within the EDN1–EDNRA pathway during early pharyngeal arch patterning. To date, two GNAI3 variants in three families have been reported. Here we report three novel GNAI3 variants, one segregating with affected members in a family previously linked to 1p21.1-...

  5. On core stability and extendability

    OpenAIRE

    Shellshear, Evan

    2007-01-01

    This paper investigates conditions under which the core of a TU cooperative game is stable. In particular the author extends the idea of extendability to find new conditions under which the core is stable. It is also shown that these new conditions are not necessary for core stability.

  6. On core stability and extendability

    OpenAIRE

    Shellshear, Evan

    2007-01-01

    This paper investigates conditions under which the core of a TU cooperative game is stable. In particular the author extends the idea of extendability to find new conditions under which the core is stable. It is also shown that these new conditions are not necessary for core stability.

  7. Extended Active Disturbance Rejection Controller

    Science.gov (United States)

    Gao, Zhiqiang (Inventor); Tian, Gang (Inventor)

    2016-01-01

    Multiple designs, systems, methods and processes for controlling a system or plant using an extended active disturbance rejection control (ADRC) based controller are presented. The extended ADRC controller accepts sensor information from the plant. The sensor information is used in conjunction with an extended state observer in combination with a predictor that estimates and predicts the current state of the plant and a co-joined estimate of the system disturbances and system dynamics. The extended state observer estimates and predictions are used in conjunction with a control law that generates an input to the system based in part on the extended state observer estimates and predictions as well as a desired trajectory for the plant to follow.

  8. Quantum Mechanics of Extended Objects

    CERN Document Server

    Sastry, R R

    2000-01-01

    We propose a quantum mechanics of extended objects that accounts for the finite extent of a particle defined via its Compton wavelength. The Hilbert space representation theory of such a quantum mechanics is presented and this representation is used to demonstrate the quantization of spacetime. The quantum mechanics of extended objects is then applied to two paradigm examples, the fuzzy (extended object) harmonic oscillator and the Yukawa potential. In the second example the phenomenological coupling constant of the $\\omega$ meson which mediates the short range and repulsive nucleon force as well as the repulsive core radius are theoretically predicted.

  9. Extended scaling in high dimensions

    Science.gov (United States)

    Berche, B.; Chatelain, C.; Dhall, C.; Kenna, R.; Low, R.; Walter, J.-C.

    2008-11-01

    We apply and test the recently proposed 'extended scaling' scheme in an analysis of the magnetic susceptibility of Ising systems above the upper critical dimension. The data are obtained by Monte Carlo simulations using both the conventional Wolff cluster algorithm and the Prokof'ev-Svistunov worm algorithm. As already observed for other models, extended scaling is shown to extend the high-temperature critical scaling regime over a range of temperatures much wider than that achieved conventionally. It allows for an accurate determination of leading and sub-leading scaling indices, critical temperatures and amplitudes of the confluent corrections.

  10. Cosmological dynamics of extended chameleons

    CERN Document Server

    Tamanini, Nicola

    2016-01-01

    We investigate the cosmological dynamics of the recently proposed extended chameleon models at both background and linear perturbation levels. Dynamical systems techniques are employed to fully characterize the evolution of the universe at the largest distances, while structure formation is analysed at sub-horizon scales within the quasi-static approximation. The late time dynamical transition from dark matter to dark energy domination can be well described by almost all extended chameleon models considered, with no deviations from $\\Lambda$CDM results at both background and perturbation levels. The results obtained in this work confirm the cosmological viability of extended chameleons as alternative dark energy models.

  11. Cognitive Transformations and Extended Expertise

    Science.gov (United States)

    Menary, Richard; Kirchhoff, Michael

    2014-01-01

    Expertise is extended by becoming immersed in cultural practices. We look at an example of mathematical expertise in which immersion in cognitive practices results in the transformation of expert performance.

  12. Extended gcd of quadratic integers

    CERN Document Server

    Miled, Abdelwaheb

    2010-01-01

    Computation of the extended gcd of two quadratic integers. The ring of integers considered is principal but could be euclidean or not euclidean ring. This method rely on principal ideal ring and reduction of binary quadratic forms.

  13. Locating and extending livelihoods research

    DEFF Research Database (Denmark)

    Prowse, Martin

    2008-01-01

    Much poverty and development research is not explicit about its methodology or philosophical foundations. Based on the extended case method of Burawoy and the epistemological standpoint of critical realism, this paper discusses a methodological approach for reflexive inductive livelihoods researc...

  14. Extended New Generalized Lindley Distribution

    Directory of Open Access Journals (Sweden)

    D. S. Shibu

    2016-03-01

    Full Text Available In this paper, we consider an extended version of new generalized Lindley distribution (NGLD. We refer to this new generalization as the extended new generalized Lindley distribution (ENGLD. A comprehensive account of the mathematical properties of the new distribution including estimation is presented. A real life data set is considered here to illustrate the relevance of the new model and compared it with other forms of Lindley models using method of moment estimation and method of maximum likelihood estimation.

  15. Extended cognition in science communication.

    Science.gov (United States)

    Ludwig, David

    2014-11-01

    The aim of this article is to propose a methodological externalism that takes knowledge about science to be partly constituted by the environment. My starting point is the debate about extended cognition in contemporary philosophy and cognitive science. Externalists claim that human cognition extends beyond the brain and can be partly constituted by external devices. First, I show that most studies of public knowledge about science are based on an internalist framework that excludes the environment we usually utilize to make sense of science and does not allow the possibility of extended knowledge. In a second step, I argue that science communication studies should adopt a methodological externalism and accept that knowledge about science can be partly realized by external information resources such as Wikipedia.

  16. An Extended Keyword Extraction Method

    Science.gov (United States)

    Hong, Bao; Zhen, Deng

    Among numerous Chinese keyword extraction methods, Chinese characteristics were shortly considered. This phenomenon going against the precision enhancement of the Chinese keyword extraction. An extended term frequency based method(Extended TF) is proposed in this paper which combined Chinese linguistic characteristics with basic TF method. Unary, binary and ternary grammars for the candidate keyword extraction as well as other linguistic features were all taken into account. The method establishes classification model using support vector machine. Tests show that the proposed extraction method improved key words precision and recall rate significantly. We applied the key words extracted by the extended TF method into the text file classification. Results show that the key words extracted by the proposed method contributed greatly to raising the precision of text file classification.

  17. EXTENDED MILD-SLOPE EQUATION

    Institute of Scientific and Technical Information of China (English)

    黄虎; 丁平兴; 吕秀红

    2001-01-01

    The Hamiltonian formalism for surface waves and the mild-slope approximation were empolyed in handling the case of slowly varying three-dimensional currents and an uneven bottom, thus leading to an extended mild-slope equation. The bottom topography consists of two components: the slowly varying component whose horizontal length scale is longer than the surface wave length, and the fast varying component with the amplitude being smaller than that of the surface wave. The frequency of the fast varying depth component is, however, comparable to that of the surface waves. The extended mild- slope equation is more widely applicable and contains as special cases famous mild-slope equations below: the classical mild-slope equation of Berkhoff , Kirby' s mild-slope equation with current, and Dingemans' s mild-slope equation for rippled bed. The extended shallow water equations for ambient currents and rapidly varying topography are also obtained.

  18. Constrained Stochastic Extended Redundancy Analysis.

    Science.gov (United States)

    DeSarbo, Wayne S; Hwang, Heungsun; Stadler Blank, Ashley; Kappe, Eelco

    2015-06-01

    We devise a new statistical methodology called constrained stochastic extended redundancy analysis (CSERA) to examine the comparative impact of various conceptual factors, or drivers, as well as the specific predictor variables that contribute to each driver on designated dependent variable(s). The technical details of the proposed methodology, the maximum likelihood estimation algorithm, and model selection heuristics are discussed. A sports marketing consumer psychology application is provided in a Major League Baseball (MLB) context where the effects of six conceptual drivers of game attendance and their defining predictor variables are estimated. Results compare favorably to those obtained using traditional extended redundancy analysis (ERA).

  19. 5-Dimensional Extended Space Model

    OpenAIRE

    Tsipenyuk, D. Yu.; Andreev, V. A.

    2006-01-01

    We put forward an idea that physical phenomena have to be treated in 5-dimensional space where the fifth coordinate is the interval S. Thus, we considered the (1+4) extended space G(T;X,Y,Z,S). In addition to Lorentz transformations (T;X), (T;Y), (T;Z) which are in (1+3)-dimensional Minkowski space, in the proposed (1+4)d extended space two other types of transformations exist in planes (T,S); (X,S), (Y,S), (Z,S) that converts massive particles into massless and vice versa. We also consider e...

  20. Group Development: Extending Tuckman's Theory.

    Science.gov (United States)

    Maples, Mary F.

    1988-01-01

    Presents a framework for extending the Tuckman model of developmental sequence in small groups. Considers Tuckman's stages of forming, storming, norming, performing, and adjourning lacking in descriptive depth and clear definition. Gathered and organized group dynamics graduate students' assessments of characteristics of stages over five-year…

  1. Applying and extending Oracle Spatial

    CERN Document Server

    Simon Gerard Greener, Siva Ravada

    2013-01-01

    This book is an advanced practical guide to applying and extending Oracle Spatial.This book is for existing users of Oracle and Oracle Spatial who have, at a minimum, basic operational experience of using Oracle or an equivalent database. Advanced skills are not required.

  2. Extended range chemical sensing apparatus

    Science.gov (United States)

    Hughes, Robert C.; Schubert, W. Kent

    1994-01-01

    An apparatus for sensing chemicals over extended range of concentrations. In particular, first and second sensors each having separate, but overlapping ranges for sensing concentrations of hydrogen are provided. Preferably, the first sensor is a MOS solid state device wherein the metal electrode or gate is a nickel alloy. The second sensor is a chemiresistor comprising a nickel alloy.

  3. Offshore Extended Drillstem Testing System

    Institute of Scientific and Technical Information of China (English)

    Yao Hao

    1995-01-01

    @@ Bohai Corp. of CNOOC has started to study and working out the offshore extended drillstem testing system(OEDST) since 1991. Now the OEDST is worked out successfully, and approved on Feb. 15,1995. OEDST has some advantages, such as simple overall program and technological process,multiple and simple operation, easy for transporation ,and low cost with efficient result.

  4. Extended duration orbiter (EDO) insignia

    Science.gov (United States)

    1990-01-01

    Extended duration orbiter (EDO) insignia incorporates a space shuttle orbiter with payload bay doors (PLBDs) open and a spacelab module inside. Trailing the orbiter are the initials EDO. The EDO-modified Columbia, Orbiter Vehicle (OV) 102, will be flown for the first EDO mission, STS-50.

  5. Extended lactation in dairy cows

    DEFF Research Database (Denmark)

    Sorensen, Annette; Muir, D. Donald; Knight, Christopher Harold

    2008-01-01

    Twelve spring-calving and twelve winter-calving cows were managed for extended lactation cycles of 18-months duration, with the former group then completing a second extended lactation. Half of the cows were fed according to standard management practice for the herd; the other half received...... of the lactation, protein and fat percentages increasing and lactose percentage decreasing, irrespective of treatment. The quality of the milk for processing into cheese, fermented products, heat-treated products and cream liqueurs was assessed by calculation of casein number (casein protein as a proportion...... of total protein). Processing quality declined across the course of lactation in those groups that showed poor persistency but not in those that maintained a persistent lactation. Milk hygienic quality (somatic cell counts) showed parallel changes. Body condition score increased during the course...

  6. Lyapunov modes in extended systems.

    Science.gov (United States)

    Yang, Hong-Liu; Radons, Günter

    2009-08-28

    Hydrodynamic Lyapunov modes, which have recently been observed in many extended systems with translational symmetry, such as hard sphere systems, dynamic XY models or Lennard-Jones fluids, are nowadays regarded as fundamental objects connecting nonlinear dynamics and statistical physics. We review here our recent results on Lyapunov modes in extended system. The solution to one of the puzzles, the appearance of good and 'vague' modes, is presented for the model system of coupled map lattices. The structural properties of these modes are related to the phase space geometry, especially the angles between Oseledec subspaces, and to fluctuations of local Lyapunov exponents. In this context, we report also on the possible appearance of branches splitting in the Lyapunov spectra of diatomic systems, similar to acoustic and optical branches for phonons. The final part is devoted to the hyperbolicity of partial differential equations and the effective degrees of freedom of such infinite-dimensional systems.

  7. Locating and extending livelihoods research

    DEFF Research Database (Denmark)

    Prowse, Martin

    2008-01-01

    Much poverty and development research is not explicit about its methodology or philosophical foundations. Based on the extended case method of Burawoy and the epistemological standpoint of critical realism, this paper discusses a methodological approach for reflexive inductive livelihoods researc...... that overcomes the unproductive social science dualism of positivism and social constructivism. The approach is linked to a conceptual framework and a menu of research methods that can be sequenced and iterated in light of research questions....

  8. An Extended Particle Swarm Optimizer

    Institute of Scientific and Technical Information of China (English)

    XU Jun-jie; XIN Zhan-hong

    2005-01-01

    An Extended Particle Swarm Optimizer (EPSO) is proposed in this paper. In this new algorithm, not only the local but also the global best position will impact the particle's velocity updating process. EPSO is an integration of Local Best paradigm (LBEST) and Global Best paradigm (GBEST) and it significantly enhances the performance of the conventional particle swarm optimizers. The experiment results have proved that EPSO deserves to be investigated.

  9. Departies: conceptualizing extended youth parties

    DEFF Research Database (Denmark)

    Fjær, Eivind Grip; Tutenges, Sébastien

    2017-01-01

    Every year, millions of young people travel away from home to party for days or weeks on end in permissive environments, such as music festivals, dance parties, and nightlife resorts. The studies that have been conducted on these extended youth parties have focused primarily on specific risk...... immoral; (4) stylistically, by altering their stylistic expressions through dress, demeanor, and consumption; and (5) experientially, because the parties generate mood and mind alterations. These are overlapping and intertwined elements, the combination of which amounts to a distinct type of youth party...

  10. Departies: conceptualizing extended youth parties

    DEFF Research Database (Denmark)

    Eivind Grip, Fjær,; Tutenges, Sébastien

    2016-01-01

    Every year, millions of young people travel away from home to party for days or weeks on end in permissive environments, such as music festivals, dance parties, and nightlife resorts. The studies that have been conducted on these extended youth parties have focused primarily on specific risk...... immoral; (4) stylistically, by altering their stylistic expressions through dress, demeanor, and consumption; and (5) experientially, because the parties generate mood and mind alterations. These are overlapping and intertwined elements, the combination of which amounts to a distinct type of youth party...

  11. Renormalization of Extended QCD$_2$

    CERN Document Server

    Fukaya, Hidenori

    2015-01-01

    Extended QCD (XQCD) proposed by Kaplan [1] is an interesting reformulation of QCD with additional bosonic auxiliary fields. While its partition function is kept exactly the same as that of original QCD, XQCD naturally contains properties of low energy hadronic models. We analyze the renormalization group flow of two-dimensional (X)QCD, which is solvable in the limit of large number of colors Nc, to understand what kind of roles the auxiliary degrees of freedom play and how the hadronic picture emerges in the low energy region.

  12. Extended UML with Role Modeling

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    UML is widely accepted and applied by the international softwareindus try. UML is a powerful language for Object-oriented modeling, designing, and i m plementing software systems, but its Use-Case method for requirement analysis a n d modeling software patterns has some explicit drawbacks. For more complete UML, this paper proposes the Role Use-Case modeling and its glyphs, and provides an instance of requirement analysis using Role Use-Case method. Uses the Role Mode l to modeling software pattern at knowledge level. This paper also extends the UM L Meta Model and accentuates “RM before UML's class Modeling”.

  13. Extended suicide with a pet.

    Science.gov (United States)

    Cooke, Brian K

    2013-01-01

    The combination of the killing of a pet and a suicide is a perplexing scenario that is largely unexplored in the literature. Many forensic psychiatrists and psychologists may be unaccustomed to considering the significance of the killing of a pet. The subject is important, however, because many people regard their pets as members of their family. A case is presented of a woman who killed her pet dog and herself by carbon monoxide poisoning. The purpose of this article is to provide an initial exploration of the topic of extended suicide with a pet. Forensic mental health evaluations may have a role in understanding the etiology of this event and in opining as to the culpability of individuals who attempt to or successfully kill a pet and then commit suicide. Because the scientific literature is lacking, there is a need to understand this act from a variety of perspectives. First, a social and anthropological perspective will be presented that summarizes the history of the practice of killing of one's pet, with a focus on the ancient Egyptians. A clinical context will examine what relationship animals have to mental illness. A vast body of existing scientific data showing the relevance of human attachment to pets suggests that conclusions from the phenomena of homicide-suicide and filicide-suicide are applicable to extended suicide with a pet. Finally, recommendations will be proposed for both clinical and forensic psychiatrists faced with similar cases.

  14. Extended recombinant bacterial ghost system.

    Science.gov (United States)

    Lubitz, W; Witte, A; Eko, F O; Kamal, M; Jechlinger, W; Brand, E; Marchart, J; Haidinger, W; Huter, V; Felnerova, D; Stralis-Alves, N; Lechleitner, S; Melzer, H; Szostak, M P; Resch, S; Mader, H; Kuen, B; Mayr, B; Mayrhofer, P; Geretschläger, R; Haslberger, A; Hensel, A

    1999-08-20

    Controlled expression of cloned PhiX174 gene E in Gram-negative bacteria results in lysis of the bacteria by formation of an E-specific transmembrane tunnel structure built through the cell envelope complex. Bacterial ghosts from a variety of bacteria are used as non-living candidate vaccines. In the recombinant ghost system, foreign proteins are attached on the inside of the inner membrane as fusions with specific anchor sequences. Ghosts have a sealed periplasmic space and the export of proteins into this space vastly extends the capacity of ghosts or recombinant ghosts to function as carriers of foreign antigens. In addition, S-layer proteins forming shell-like self assembly structures can be expressed in candidate vaccine strains prior to E-mediated lysis. Such recombinant S-layer proteins carrying foreign epitopes further extend the possibilities of ghosts as carriers of foreign epitopes. As ghosts have inherent adjuvant properties, they can be used as adjuvants in combination with subunit vaccines. Subunits or other ligands can also be coupled to matrixes like dextran which are used to fill the internal lumen of ghosts. Oral, aerogenic or parenteral immunization of experimental animals with recombinant ghosts induced specific humoral and cellular immune responses against bacterial and target components including protective mucosal immunity. The most relevant advantage of recombinant bacterial ghosts as immunogens is that no inactivation procedures that denature relevant immunogenic determinants are employed in this production. This fact explains the superior quality of ghosts when compared to other inactivated vaccines. The endotoxic component of the outer membrane does not limit the use of ghosts as vaccine candidates but triggers the release of several potent immunoregulatory cytokines. As carriers, there is no limitation in the size of foreign antigens that can be inserted in the membrane and the capacity of all spaces including the membranes, peri

  15. Dicyanometallates as Model Extended Frameworks

    Science.gov (United States)

    2016-01-01

    We report the structures of eight new dicyanometallate frameworks containing molecular extra-framework cations. These systems include a number of hybrid inorganic–organic analogues of conventional ceramics, such as Ruddlesden–Popper phases and perovskites. The structure types adopted are rationalized in the broader context of all known dicyanometallate framework structures. We show that the structural diversity of this family can be understood in terms of (i) the charge and coordination preferences of the particular metal cation acting as framework node, and (ii) the size, shape, and extent of incorporation of extra-framework cations. In this way, we suggest that dicyanometallates form a particularly attractive model family of extended frameworks in which to explore the interplay between molecular degrees of freedom, framework topology, and supramolecular interactions. PMID:27057759

  16. Hamiltonian dynamics of extended objects

    Energy Technology Data Exchange (ETDEWEB)

    Capovilla, R [Departamento de FIsica, Centro de Investigacion y de Estudios Avanzados del IPN, Apdo Postal 14-740, 07000 Mexico, DF (Mexico); Guven, J [School of Theoretical Physics, Dublin Institute for Advanced Studies, 10 Burlington Road, Dublin 4 (Ireland); Rojas, E [Instituto de Ciencias Nucleares, Universidad Nacional Autonoma de Mexico, Apdo Postal 70-543, 04510 Mexico, DF (Mexico)

    2004-12-07

    We consider relativistic extended objects described by a reparametrization-invariant local action that depends on the extrinsic curvature of the worldvolume swept out by the object as it evolves. We provide a Hamiltonian formulation of the dynamics of such higher derivative models which is motivated by the ADM formulation of general relativity. The canonical momenta are identified by looking at boundary behaviour under small deformations of the action; the relationship between the momentum conjugate to the embedding functions and the conserved momentum density is established. The canonical Hamiltonian is constructed explicitly; the constraints on the phase space, both primary and secondary, are identified and the role they play in the theory is described. The multipliers implementing the primary constraints are identified in terms of the ADM lapse and shift variables and Hamilton's equations are shown to be consistent with the Euler-Lagrange equations.

  17. Learning Extended Finite State Machines

    Science.gov (United States)

    Cassel, Sofia; Howar, Falk; Jonsson, Bengt; Steffen, Bernhard

    2014-01-01

    We present an active learning algorithm for inferring extended finite state machines (EFSM)s, combining data flow and control behavior. Key to our learning technique is a novel learning model based on so-called tree queries. The learning algorithm uses the tree queries to infer symbolic data constraints on parameters, e.g., sequence numbers, time stamps, identifiers, or even simple arithmetic. We describe sufficient conditions for the properties that the symbolic constraints provided by a tree query in general must have to be usable in our learning model. We have evaluated our algorithm in a black-box scenario, where tree queries are realized through (black-box) testing. Our case studies include connection establishment in TCP and a priority queue from the Java Class Library.

  18. Object-Extended OLAP Querying

    DEFF Research Database (Denmark)

    Pedersen, Torben Bach; Gu, Junmin; Shoshani, Arie

    2009-01-01

    inherent in data in non-standard applications are not accommodated well by OLAP systems. In contrast, object database systems are built to handle such complexity, but do not support OLAP-type querying well. This paper presents the concepts and techniques underlying a flexible, "multi-model" federated...... system that enables OLAP users to exploit simultaneously the features of OLAP and object systems. The system allows data to be handled using the most appropriate data model and technology: OLAP systems for dimensional data and object database systems for more complex, general data. This allows data...... analysis on the OLAP data to be significantly enriched by the use of additional object data. Additionally, physical integration of the OLAP and the object data can be avoided. As a vehicle for demonstrating the capabilities of the system, a prototypical OLAP language is defined and extended to naturally...

  19. The Limits of Extended Quintessence

    CERN Document Server

    Nesseris, S

    2006-01-01

    We use a low redshift expansion of the cosmological equations of extended (scalar-tensor) quintessence to divide the observable Hubble history parameter space in four sectors: A forbidden sector I where the scalar field of the theory becomes imaginary (the kinetic term becomes negative), a forbidden sector II where the scalar field rolls up (instead of down) its potential, an allowed `freezing' quintessence sector III where the scalar field is currently decelerating down its potential towards freezing and an allowed `thawing' sector IV where the scalar field is currently accelerating down its potential. The dividing lines between the sectors depend sensitively on the time derivatives of the Newton's constant G over powers of the Hubble parameter. For minimally coupled quintessence which appears as a special case for a constant G our results are consistent with previous studies. Observable parameter \\chi^2 contours based on current data (SNLS dataset) are also constructed on top of the sectors, for a prior of ...

  20. Extended class of phenomenological universalities

    Science.gov (United States)

    Molski, Marcin

    2017-08-01

    The phenomenological universalities (PU) are extended to include quantum oscillatory phenomena, coherence and supersymmetry. It will be proved that this approach generates minimum uncertainty coherent states of time-dependent oscillators, which in the dissociation (classical) limit reduce to the functions describing growth (regression) of the systems evolving over time. The PU formalism can be applied also to construct the coherent states of space-dependent oscillators, which in the dissociation limit produce cumulative distribution functions widely used in probability theory and statistics. A combination of the PU and supersymmetry provides a convenient tool for generating analytical solutions of the Fokker-Planck equation with the drift term related to the different forms of potential energy function. The results obtained reveal existence of a new class of macroscopic quantum (or quasi-quantum) phenomena, which may play a vital role in coherent formation of the specific growth patterns in complex systems.

  1. Extended Klein edges in graphene.

    Science.gov (United States)

    He, Kuang; Robertson, Alex W; Lee, Sungwoo; Yoon, Euijoon; Lee, Gun-Do; Warner, Jamie H

    2014-12-23

    Graphene has three experimentally confirmed periodic edge terminations, zigzag, reconstructed 5-7, and arm-chair. Theory predicts a fourth periodic edge of graphene called the extended Klein (EK) edge, which consists of a series of single C atoms protruding from a zigzag edge. Here, we confirm the existence of EK edges in both graphene nanoribbons and on the edge of bulk graphene using atomic resolution imaging by aberration-corrected transmission electron microscopy. The formation of the EK edge stems from sputtering and reconstruction of the zigzag edge. Density functional theory reveals minimal energy for EK edge reconstruction and bond distortion both in and out of plane, supporting our TEM observations. The EK edge can now be included as the fourth member of observed periodic edge structures in graphene.

  2. THE EXTENDED VIRGO CLUSTER CATALOG

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Suk; Rey, Soo-Chang; Lee, Youngdae; Chung, Jiwon; Pak, Mina; Yi, Wonhyeong; Lee, Woong [Department of Astronomy and Space Science, Chungnam National University, 99 Daehak-ro, Daejeon 305-764 (Korea, Republic of); Jerjen, Helmut [Research School of Astronomy and Astrophysics, The Australian National University, Cotter Road, Weston, ACT 2611 (Australia); Lisker, Thorsten [Astronomisches Rechen-Institut, Zentrum für Astronomie der Universität Heidelberg (ZAH), Mönchhofstraße 12-14, D-69120 Heidelberg (Germany); Sung, Eon-Chang [Korea Astronomy and Space Science institute, 776 Daedeokdae-ro, Daejeon 305-348 (Korea, Republic of)

    2015-01-01

    We present a new catalog of galaxies in the wider region of the Virgo cluster, based on the Sloan Digital Sky Survey (SDSS) Data Release 7. The Extended Virgo Cluster Catalog (EVCC) covers an area of 725 deg{sup 2} or 60.1 Mpc{sup 2}. It is 5.2 times larger than the footprint of the classical Virgo Cluster Catalog (VCC) and reaches out to 3.5 times the virial radius of the Virgo cluster. We selected 1324 spectroscopically targeted galaxies with radial velocities less than 3000 km s{sup –1}. In addition, 265 galaxies that have been overlooked in the SDSS spectroscopic survey but have available redshifts in the NASA Extragalactic Database are also included. Our selection process secured a total of 1589 galaxies, 676 of which are not included in the VCC. The certain and possible cluster members are defined by means of redshift comparison with a cluster infall model. We employed two independent and complementary galaxy classification schemes: the traditional morphological classification based on the visual inspection of optical images and a characterization of galaxies from their spectroscopic features. SDSS u, g, r, i, and z passband photometry of all EVCC galaxies was performed using Source Extractor. We compare the EVCC galaxies with the VCC in terms of morphology, spatial distribution, and luminosity function. The EVCC defines a comprehensive galaxy sample covering a wider range in galaxy density that is significantly different from the inner region of the Virgo cluster. It will be the foundation for forthcoming galaxy evolution studies in the extended Virgo cluster region, complementing ongoing and planned Virgo cluster surveys at various wavelengths.

  3. Technological Packages Extended To Farmers by Non ...

    African Journals Online (AJOL)

    Global Approaches to Extension Practice: A Journal of Agricultural Extension ... crops were the significant crop production technologies extended in the study area. ... Modern snailery was the only identified agro forestry technology extended, ...

  4. Extending Characters on Fix Algebras

    CERN Document Server

    Wagner, Stefan

    2011-01-01

    A dynamical system is a triple $(A,G,\\alpha)$, consisting of a unital locally convex algebra $A$, a topological group $G$ and a group homomorphism $\\alpha:G\\rightarrow\\Aut(A)$, which induces a continuous action of $G$ on $A$. Further, a unital locally convex algebra $A$ is called continuous inverse algebra, or CIA for short, if its group of units $A^{\\times}$ is open in $A$ and the inversion $\\iota:A^{\\times}\\rightarrow A^{\\times},\\,\\,\\,a\\mapsto a^{-1}$ is continuous at $1_A$. For a compact manifold $M$, the Fr\\'echet algebra of smooth functions $C^{\\infty}(M)$ is the prototype of such a continuous inverse algebra. We show that if $A$ is a complete commutative CIA, $G$ a compact group and $(A,G,\\alpha)$ a dynamical system, then each character of $B:=A^G$ can be extended to a character of $A$. In particular, the natural map on the level of the corresponding spectra $\\Gamma_A\\rightarrow\\Gamma_B$, $\\chi\\mapsto\\chi_{\\mid B}$ is surjective.

  5. The motivation behind extended suicide

    Directory of Open Access Journals (Sweden)

    Roman Kuruc

    2014-12-01

    Full Text Available The investigation of motivation of suicidal behaviour in cases of so-called extended suicide is of great importance from a forensic-psychological viewpoint. The initiator of such action is often a person suffering from endogenous depression. The motives behind successful suicide are generally not known. This paper aims to demonstrate the motives behind the successful suicide, less frequent in our conditions, of two family members – a father and a son. The case has been thoroughly analysed by morphological methods with the help of additional laboratory tests. At the centre was a suicide letter which was hidden in a very unusual way and which was elucidated only thanks to autopsy of both persons. The manner of realisation – hanging – was among the most frequent in the region and in the state too. The men were not under the influence of any toxic substances. The motivation behind the suicide was an escape from hard living conditions.

  6. CPHD filter derivation for extended targets

    CERN Document Server

    Orguner, Umut

    2010-01-01

    This document derives the CPHD filter for extended targets. Only the update step is derived here. Target generated measurements, false alarms and prior are all assumed to be independent identically distributed cluster processes. We also prove here that the derived CPHD filter for extended targets reduce to PHD filter for extended targets and CPHD filter for standard targets under suitable assumptions.

  7. Two Methods for Extending Quantum Key Warehouse

    Institute of Scientific and Technical Information of China (English)

    WU Min; LIAO Chang-jun; LIU Song-hao

    2006-01-01

    Because the rates of quantum key distribution systems are too low,the interleaving technique and interpolation technique are used to extend the capacity of the quantum key warehouse to increase the quantum key rates of quantum secure communication systems. the simulation technique can extend random sequences and that their randomness are invariable. The correlative theory and technique of digital singal processing is an effective method of extending the quantum dey warehouse.

  8. The X-33 Extended Flight Test Range

    Science.gov (United States)

    Mackall, Dale A.; Sakahara, Robert; Kremer, Steven E.

    1998-01-01

    Development of an extended test range, with range instrumentation providing continuous vehicle communications, is required to flight-test the X-33, a scaled version of a reusable launch vehicle. The extended test range provides vehicle communications coverage from California to landing at Montana or Utah. This paper provides an overview of the approaches used to meet X-33 program requirements, including using multiple ground stations, and methods to reduce problems caused by reentry plasma radio frequency blackout. The advances used to develop the extended test range show other hypersonic and access-to-space programs can benefit from the development of the extended test range.

  9. Extending models for two-dimensional constraints

    DEFF Research Database (Denmark)

    Forchhammer, Søren

    2009-01-01

    Random fields in two dimensions may be specified on 2 times 2 elements such that the probabilities of finite configurations and the entropy may be calculated explicitly. The Pickard random field is one example where probability of a new (non-boundary) element is conditioned on three previous...... elements. To extend the concept we consider extending such a field such that a vector or block of elements is conditioned on a larger set of previous elements. Given a stationary model defined on 2 times 2 elements, iterative scaling is used to define the extended model. The extended model may be used...

  10. Lévy Bases and Extended Subordination

    DEFF Research Database (Denmark)

    Barndorff-Nielsen, Ole

    The concept of subordination of Lévy processes is reinterpreted and then extended to a definition of subordination of Lévy bases. This is extended a step further, and then applied to give an alternative way of volatility/intermittency modulation in the context of ambit fields.......The concept of subordination of Lévy processes is reinterpreted and then extended to a definition of subordination of Lévy bases. This is extended a step further, and then applied to give an alternative way of volatility/intermittency modulation in the context of ambit fields....

  11. Report on Extended Campus Library Services.

    Science.gov (United States)

    Strickler, Sally Ann; And Others

    This report presents the results of a study of the Extended Campus Library Services program at Western Kentucky University (WKU), which was conducted by a committee appointed by the Director of University Libraries. A description of the program's goals and objectives is followed by a review of the extended campus programs in relation to similar…

  12. 38 CFR 21.74 - Extended evaluation.

    Science.gov (United States)

    2010-07-01

    ...) VOCATIONAL REHABILITATION AND EDUCATION Vocational Rehabilitation and Employment Under 38 U.S.C. Chapter 31 Duration of Rehabilitation Programs § 21.74 Extended evaluation. (a) General. An extended evaluation may be... concurrence of the Vocational Rehabilitation and Employment (VR&E) Officer before approving the extension of...

  13. Evaluation of Kentucky's Extended School Services Program.

    Science.gov (United States)

    Meehan, Merrill L.; Cowley, Kimberly S.; Schumacher, Debbie; Hauser, Brenda

    The Extended School Services (ESS) program was established in 1990 as part of the Kentucky Education Reform Act. The program extends the school day, week, or year for students at risk of academic failure, providing them with additional instructional time to help them meet academic goals. An evaluation of ESS in 2001 utilized statewide surveys and…

  14. Big Gods: Extended prosociality or group binding?

    Science.gov (United States)

    Galen, Luke W

    2016-01-01

    Big Gods are described as having a "prosocial" effect. However, this conflates parochialism (group cohesion) with cooperation extended to strangers or out-group members. An examination of the cited experimental studies indicates that religion is actually associated with increased within-group parochialism, rather than extended or universal prosociality, and that the same general mechanisms underlie both religious and secular effects.

  15. Teaching the Extended Modifier: A Transformational Approach.

    Science.gov (United States)

    Mann, G. Thomas

    1985-01-01

    Offers a practical model for teaching the extended modifier that is economical and linguistically sound, based on the insights and methodology of transformational generative grammar. Five transformational rules are given for generating extended modifiers from relative clauses. Examples of transformations and two class exercises in German are also…

  16. Extended, Embodied Cognition and Second Language Acquisition

    Science.gov (United States)

    Atkinson, Dwight

    2010-01-01

    A "cognitivist" approach to cognition has traditionally dominated second language acquisition (SLA) studies. In this article, I examine two alternative approaches--"extended cognition" and "embodied cognition"--for how they might help us conceptualize SLA. More specifically, I present: (i) summaries of extended and embodied cognition, followed by…

  17. Extended BRST quantization in general coordinates

    CERN Document Server

    Geyer, B; Nersessian, A B

    2002-01-01

    We propose an extended BRST invariant Lagrangian quantization scheme of general gauge theories based on explicit realization of "modified triplectic algebra" in general coordinates. All the known Lagrangian quantization schemes based on the extended BRST symmetry are obtained by specifying the (free) parameters of that method.

  18. Extended, Embodied Cognition and Second Language Acquisition

    Science.gov (United States)

    Atkinson, Dwight

    2010-01-01

    A "cognitivist" approach to cognition has traditionally dominated second language acquisition (SLA) studies. In this article, I examine two alternative approaches--"extended cognition" and "embodied cognition"--for how they might help us conceptualize SLA. More specifically, I present: (i) summaries of extended and embodied cognition, followed by…

  19. 42 CFR 488.310 - Extended survey.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Extended survey. 488.310 Section 488.310 Public...) STANDARDS AND CERTIFICATION SURVEY, CERTIFICATION, AND ENFORCEMENT PROCEDURES Survey and Certification of Long-Term Care Facilities § 488.310 Extended survey. (a) Purpose of survey. The purpose of an...

  20. Constructing Extended Formulations from Reflection Relations

    CERN Document Server

    Kaibel, Volker

    2010-01-01

    There are many examples of optimization problems whose associated polyhedra can be described much nicer, and with way less inequalities, by projections of higher dimensional polyhedra than this would be possible in the original space. However, currently not many general tools to construct such extended formulations are available. In this paper, we develop a framework of polyhedral relations that generalizes inductive constructions of extended formulations via projections, and we particularly elaborate on the special case of reflection relations. The latter ones provide polynomial size extended formulations for several polytopes that can be constructed as convex hulls of the unions of (exponentially) many copies of an input polytope obtained via sequences of reflections at hyperplanes. We demonstrate the use of the framework by deriving small extended formulations for the G-permutahedra of all finite reflection groups G (generalizing both Goeman's extended formulation of the permutahedron of size O(n log n) an...

  1. Expanding the prion concept to cancer biology: dominant-negative effect of aggregates of mutant p53 tumour suppressor

    OpenAIRE

    2013-01-01

    p53 is a key protein that participates in cell-cycle control, and its malfunction can lead to cancer. This tumour suppressor protein has three main domains; the N-terminal transactivation domain, the CTD (C-terminal domain) and the core domain (p53C) that constitutes the sequence-specific DBD (DNA-binding region). Most p53 mutations related to cancer development are found in the DBD. Aggregation of p53 into amyloid oligomers and fibrils has been shown. Moreover, amyloid aggregates of both the...

  2. Molecular interaction of connexin 30.3 and connexin 31 suggests a dominant-negative mechanism associated with erythrokeratodermia variabilis

    DEFF Research Database (Denmark)

    Plantard, Laure; Huber, Marcel; Macari, Francoise

    2003-01-01

    to a gigantic increase of stabilized heteromeric gap junctions. Furthermore, co-expressed wild-type Cx30.3 and Cx31 coprecipitate, which demonstrates a physical interaction. Inhibitor experiments revealed that this interaction begins in the endoplasmic reticulum. These results not only provide new insights...

  3. A novel radiation-induced p53 mutation is not implicated in radiation resistance via a dominant-negative effect.

    Directory of Open Access Journals (Sweden)

    Yunguang Sun

    Full Text Available Understanding the mutations that confer radiation resistance is crucial to developing mechanisms to subvert this resistance. Here we describe the creation of a radiation resistant cell line and characterization of a novel p53 mutation. Treatment with 20 Gy radiation was used to induce mutations in the H460 lung cancer cell line; radiation resistance was confirmed by clonogenic assay. Limited sequencing was performed on the resistant cells created and compared to the parent cell line, leading to the identification of a novel mutation (del at the end of the DNA binding domain of p53. Levels of p53, phospho-p53, p21, total caspase 3 and cleaved caspase 3 in radiation resistant cells and the radiation susceptible (parent line were compared, all of which were found to be similar. These patterns held true after analysis of p53 overexpression in H460 cells; however, H1299 cells transfected with mutant p53 did not express p21, whereas those given WT p53 produced a significant amount, as expected. A luciferase assay demonstrated the inability of mutant p53 to bind its consensus elements. An MTS assay using H460 and H1299 cells transfected with WT or mutant p53 showed that the novel mutation did not improve cell survival. In summary, functional characterization of a radiation-induced p53 mutation in the H460 lung cancer cell line does not implicate it in the development of radiation resistance.

  4. The effect of losartan therapy on ventricular function in Marfan patients with haploinsufficient or dominant negative FBN1 mutations

    NARCIS (Netherlands)

    den Hartog, A. W.; Franken, R.; van den Berg, M. P.; Zwinderman, A. H.; Timmermans, J.; Scholte, A. J.; de Waard, V.; Spijkerboer, A. M.; Pals, G.; Mulder, B. J. M.; Groenink, M.

    2016-01-01

    Background Mild biventricular dysfunction is often present in patients with Marfan syndrome. Losartan has been shown to reduce aortic dilatation in patients with Marfan syndrome. This study assesses the effect of losartan on ventricular volume and function in genetically classified subgroups of asym

  5. A dominant negative erythropoietin (EPO) receptor inhibits EPO-dependent growth and blocks F-gp55-dependent transformation.

    OpenAIRE

    Barber, D L; DeMartino, J C; Showers, M O; D'Andrea, A D

    1994-01-01

    The erythropoietin receptor (EPO-R), a member of the cytokine receptor superfamily, can be activated to signal cell growth by binding either EPO or F-gp55, the Friend spleen focus-forming virus glycoprotein. Activation by F-gp55 results in constitutive EPO-R signalling and the first stage of Friend virus-induced erythroleukemia. We have generated a truncated form of the EPO-R polypeptide [EPO-R(T)] which lacks the critical cytoplasmic signal-transducing domain of the EPO-R required for EPO- o...

  6. A dominant negative mutant of TLK1 causes chromosome missegregation and aneuploidy in normal breast epithelial cells

    Directory of Open Access Journals (Sweden)

    Williams Briana

    2003-10-01

    Full Text Available Abstract Background In Arabidopsis thaliana, the gene Tousled encodes a protein kinase of unknown function, but mutations in the gene lead to flowering and leaf morphology defects. We have recently cloned a mammalian Tousled-Like Kinase (TLK1B and found that it phosphorylates specifically histone H3, in vitro and in vivo. We now report the effects that overexpression of a kinase-dead mutant of TLK1B mediates in a normal diploid cell line. Results Expression of a kinase-dead mutant resulted in reduction of phosphorylated histone H3, which could have consequences in mitotic segregation of chromosomes. When analyzed by FACS and microscopy, these cells displayed high chromosome number instability and aneuploidy. This phenomenon was accompanied by less condensed chromosomes at mitosis; failure of a number of chromosomes to align properly on the metaphase plate; failure of some chromosomes to attach to microtubules; and the occasional presentation of two bipolar spindles. We also used a different method (siRNA to reduce the level of endogenous TLK1, but in this case, the main result was a strong block of cell cycle progression suggesting that TLK1 may also play a role in progression from G1. This block in S phase progression could also offer a different explanation of some of the later mitotic defects. Conclusions TLK1 has a function important for proper chromosome segregation and maintenance of diploid cells at mitosis in mammalian cells that could be mediated by reduced phosphorylation of histone H3 and condensation of chromosomes, although other explanations to the phenotype are possible.

  7. The effect of losartan therapy on ventricular function in Marfan patients with haploinsufficient or dominant negative FBN1 mutations

    NARCIS (Netherlands)

    Hartog, A.W. den; Franken, R.; Berg, M.P.; Zwinderman, A.H.; Timmermans, J.; Scholte, A.J.; Waard, V. de; Spijkerboer, A.M.; Pals, G.; Mulder, B.J.; Groenink, M.

    2016-01-01

    BACKGROUND: Mild biventricular dysfunction is often present in patients with Marfan syndrome. Losartan has been shown to reduce aortic dilatation in patients with Marfan syndrome. This study assesses the effect of losartan on ventricular volume and function in genetically classified subgroups of asy

  8. The effect of losartan therapy on ventricular function in Marfan patients with haploinsufficient or dominant negative FBN1 mutations

    NARCIS (Netherlands)

    den Hartog, A. W.; Franken, R.; van den Berg, M. P.; Zwinderman, A. H.; Timmermans, J.; Scholte, A. J.; de Waard, V.; Spijkerboer, A. M.; Pals, G.; Mulder, B. J. M.; Groenink, M.

    2016-01-01

    Background Mild biventricular dysfunction is often present in patients with Marfan syndrome. Losartan has been shown to reduce aortic dilatation in patients with Marfan syndrome. This study assesses the effect of losartan on ventricular volume and function in genetically classified subgroups of asym

  9. Soluble dominant-negative receptor uncovers essential roles for fibroblast growth factors in multi-organ induction and patterning.

    OpenAIRE

    Celli, G; LaRochelle, W J; Mackem, S; Sharp, R.; Merlino, G.

    1998-01-01

    Despite a wealth of experimental data implicating fibroblast growth factor (FGF) signaling in various developmental processes, genetic inactivation of individual genes encoding specific FGFs or their receptors (FGFRs) has generally failed to demonstrate their role in vertebrate organogenesis due to early embryonic lethality or functional redundancy. Here we show that broad mid-gestational expression of a novel secreted kinase-deficient receptor, specific for a defined subset of the FGF superf...

  10. Naturally occurring Bruton's tyrosine kinase mutations have no dominant negative effect in an X-linked agammaglobulinaemia cellular model

    Science.gov (United States)

    Pérez de Diego, R; López-Granados, E; Rivera, J; Ferreira, A; Fontán, G; Bravo, J; García Rodríguez, MC; Bolland, S

    2008-01-01

    X-linked agammaglobulinaemia (XLA) is characterized by absence of mature B cells because of mutations in the Bruton's tyrosine kinase (Btk) gene. Btk-deficient early B cell precursors experience a block in their differentiation potentially reversible by the addition of an intact Btk gene. Btk expression was measured in 69 XLA patients with 47 different mutations and normal expression was detected in seven. We characterized these Btk mutant forms functionally by transfection into a lymphoma cell line that lacks endogenous Btk expression (Btk−/− DT40 cells) and analysed the calcium flux in response to B cell receptor stimulation. To test whether co-expression of a mutated form could compromise the function of the intact Btk transfection, studies in wild-type (WT) DT40 cells were also performed. Study reveals that none of the seven Btk mutants analysed was able to revert the absence of calcium mobilization upon IgM engagement in Btk−/− DT40 cells, as does intact Btk. In addition, calcium mobilization by anti-IgM stimulation in DT40 Btk+/+ cells was unaffected by co-expression with Btk mutants. These results suggest that gene addition would be feasible not only for patients with XLA and mutations that prevent Btk expression, but for those with expression of a mutant Btk. PMID:18241233

  11. The symmetric extendibility of quantum states

    Science.gov (United States)

    Nowakowski, Marcin L.

    2016-09-01

    Studies on the symmetric extendibility of quantum states have become particularly important in the context of the analysis of one-way quantum measures of entanglement, and the distillability and security of quantum protocols. In this paper we analyze composite systems containing a symmetric extendible part, with particular attention devoted to the one-way security of such systems. Further, we introduce a new one-way entanglement monotone based on the best symmetric approximation of a quantum state and the extendible number of a quantum state. We underpin these results with geometric observations about the structures of multi-party settings which posses substantial symmetric extendible components in their subspaces. The impossibility of reducing the maximal symmetric extendibility by means of the one-way local operations and classical communication method is pointed out on multiple copies. Finally, we state a conjecture linking symmetric extendibility with the one-way distillability and security of all quantum states, analyzing the behavior of a private key in the neighborhood of symmetric extendible states.

  12. Extended Cognitive System and Epistemic Subject

    Directory of Open Access Journals (Sweden)

    Trybulec Barbara

    2015-03-01

    Full Text Available The concept of an extended cognitive system is central to contemporary studies of cognition. In the paper I analyze the place of the epistemic subject within the extended cognitive system. Is it extended as well? In answering this question I focus on the differences between the first and the second wave of arguments for the extended mind thesis. I argue that the position of Cognitive Integration represented by Richard Menary is much more intuitive and fruitful in analyses of cognition and knowledge than the early argument formulated by Andy Clark and David Chalmers. Cognitive Integration is compatible with virtue epistemology of John Greco’s agent reliabilism. The epistemic subject is constituted by its cognitive character composed of an integrated set of cognitive abilities and processes. Some of these processes are extended, they are a manipulation of external informational structures and, as such, they constitute epistemic practices. Epistemic practices are normative; to conduct them correctly the epistemic subject needs to obey epistemic norms embedded in the cultural context. The epistemic subject is not extended because of the casual coupling with external informational artifacts which extend his mind from inside the head and into the world. Rather, cognitive practices constitute the subject’s mind, they transform his cognitive abilities, and this is what makes the mind and epistemic subject “extended”.

  13. Extended Hamiltonian approach to continuous tempering.

    Science.gov (United States)

    Gobbo, Gianpaolo; Leimkuhler, Benedict J

    2015-06-01

    We introduce an enhanced sampling simulation technique based on continuous tempering, i.e., on continuously varying the temperature of the system under investigation. Our approach is mathematically straightforward, being based on an extended Hamiltonian formulation in which an auxiliary degree of freedom, determining the effective temperature, is coupled to the physical system. The physical system and its temperature evolve continuously in time according to the equations of motion derived from the extended Hamiltonian. Due to the Hamiltonian structure, it is easy to show that a particular subset of the configurations of the extended system is distributed according to the canonical ensemble for the physical system at the correct physical temperature.

  14. Supervisory Control of Extended Timed Event Graphs

    Institute of Scientific and Technical Information of China (English)

    Zhi-bing Zhuo; Wen-de Chen

    2003-01-01

    This paper describes the dynamic behavior of extended timed event graphs related to place delay in the dioid framework. By Cofer and Garg's supervisory control theory[3], we address control problems of extended timed event graphs. Supervisory control of extended timed event graphs (a class of discrete event dynamic systems) is studied in the dioid framework, a necessary and sufficient condition for the ideals of the set of firing time sequences of transitions to be controllable is presented. We prove all the strongly controllable subsets can form a complete lattice.

  15. Superfield Lagrangian Quantization with Extended BRST Symmetry

    CERN Document Server

    Lavrov, P M

    2001-01-01

    We consider possible superfield representations of extended BRST symmetry for general gauge theories within the principle of gauge-fixing based on a generating equation for the gauge functional. We examine admissible superfield choices for an extended antibracket and delta-operator with given algebraic properties and show that only one of these choices is compatible with the requirement of extended BRST symmetry realized in terms of supertranslations along Grassmann coordinates. We demonstrate that this realization leads to the gauge-independence of the S-matrix.

  16. The extended loop representation of quantum gravity

    CERN Document Server

    Di Bartolo, C; Griego, J R

    1995-01-01

    A new representation of Quantum Gravity is developed. This formulation is based on an extension of the group of loops. The enlarged group, that we call the Extended Loop Group, behaves locally as an infinite dimensional Lie group. Quantum Gravity can be realized on the state space of extended loop dependent wavefunctions. The extended representation generalizes the loop representation and contains this representation as a particular case. The resulting diffeomorphism and hamiltonian constraints take a very simple form and allow to apply functional methods and simplify the loop calculus. In particular we show that the constraints are linear in the momenta. The nondegenerate solutions known in the loop representation are also solutions of the constraints in the new representation. The practical calculation advantages allows to find a new solution to the Wheeler-DeWitt equation. Moreover, the extended representation puts in a precise framework some of the regularization problems of the loop representation. We sh...

  17. Characterising and modelling extended conducted electromagnetic emission

    CSIR Research Space (South Africa)

    Grobler, Inus

    2013-06-01

    Full Text Available -1 2013 IEEE Energy Conversion Congress and Exposition Asia (ECCE Downunder), Melbourne, Australia, 3-6 June 2013 Characterising and Modelling Extended Conducted Electromagnetic Emission I Grobler1 and MN Gitau2 Department of Electrical...

  18. Extended Reconstructed Sea Surface Temperature (ERSST)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Extended Reconstructed Sea Surface Temperature (ERSST) dataset is a global monthly sea surface temperature analysis derived from the International Comprehensive...

  19. Duality Covariant Solutions in Extended Field Theories

    CERN Document Server

    Rudolph, Felix J

    2016-01-01

    Double field theory and exceptional field theory are formulations of supergravity that make certain dualities manifest symmetries of the action. To achieve this, the geometry is extended by including dual coordinates corresponding to winding modes of the fundamental objects. This geometrically unifies the spacetime metric and the gauge fields (and their local symmetries) in a generalized geometry. Solutions to these extended field theories take the simple form of waves and monopoles in the extended space. From a supergravity point of view they appear as 1/2 BPS objects such as the string, the membrane and the fivebrane in ordinary spacetime. In this thesis double field theory and exceptional field theory are introduced, solutions to their equations of motion are constructed and their properties are analyzed. Further it is established how isometries in the extended space give rise to duality relations between the supergravity solutions. Extensions to these core ideas include studying Goldstone modes, probing s...

  20. Extended emission associated with young HII regions

    CERN Document Server

    Ellingsen, S P; Kurtz, S E

    2004-01-01

    We have used the Australia Telescope Compact Array (ATCA) to make observations of a sample of eight young ultra-compact HII regions, selected on the basis that they have associated class II methanol maser emission. We have made observations sensitive to both compact and extended structures and find both to be present in most sources. The scale of the extended emission in our sample is in general less than that observed towards samples based on IRAS properties, or large single-dish flux densities. Our observations are consistent with a scenario where extended and compact radio continuum emission coexists within HII regions for a significant period of time. We suggest that these observations are consistent with a model where HII evolution takes place within hierarchically structured molecular clouds. This model is the subject of a companion paper (Shabala et al. 2005) and addresses both the association between compact and extended emission and UCHII region lifetime problem.

  1. Extending Office 365 with Microsoft Azure

    OpenAIRE

    Piirainen, Ville

    2016-01-01

    The topic of this thesis is extending Office 365 with cloud services offered by Microsoft Azure. The purpose of this thesis is to identify and present Azure services which can be used to extend Office 365 functionality. The most essential services and tools are covered from built-in services to custom solutions. In addition to Azure services, Office 365 application programming interfaces are also covered as they can be used with several extension scenarios. Both Office 365 and Azure are r...

  2. The Extended Family and Children's Educational Success

    DEFF Research Database (Denmark)

    Jæger, Mads Meier

    2012-01-01

    Research on family background and educational success focuses almost exclusively on two generations and on parents and children. This paper argues that the extended family makes up a significant part of the total effect of family background on educational success. Empirical results based on the W...... on educational success originates in the immediate family, the extended family and in interactions between these two family environments....

  3. Extended Dislocations in Plastically Deformed Metallic Nanoparticles

    Directory of Open Access Journals (Sweden)

    Bin Zheng

    2016-05-01

    Full Text Available In the present study, the sawtooth nature of compressive loading of metallic nanoparticles is observed using a molecular dynamics simulation. The atomic structure evolution confirmed that extended dislocations are the main defects split into two asynchronous partial disloca‐ tions, along with stored and released fault energy. This is considered the essence of sawtooth loading. The size of the nanoparticles relative to the equilibrium width of the extended dislocation is discussed to explain the simulation results.

  4. Some Aspects of Extended Kinetic Equation

    Directory of Open Access Journals (Sweden)

    Dilip Kumar

    2015-09-01

    Full Text Available Motivated by the pathway model of Mathai introduced in 2005 [Linear Algebra and Its Applications, 396, 317–328] we extend the standard kinetic equations. Connection of the extended kinetic equation with fractional calculus operator is established. The solution of the general form of the fractional kinetic equation is obtained through Laplace transform. The results for the standard kinetic equation are obtained as the limiting case.

  5. Musical Manipulations and the Emotionally Extended Mind

    Directory of Open Access Journals (Sweden)

    Joel Krueger

    2015-05-01

    Full Text Available I respond to Kersten's criticism in his article "Music and Cognitive Extension" of my approach to the musically extended emotional mind in Krueger (2014. I specify how we manipulate—and in so doing, integrate with—music when, as active listeners, we become part of a musically extended cognitive system. I also indicate how Kersten's account might be enriched by paying closer attention to the way that music functions as an environmental artifact for emotion regulation.

  6. IMM Iterated Extended Particle Filter Algorithm

    OpenAIRE

    Yang Wan; Shouyong Wang; Xing Qin

    2013-01-01

    In order to solve the tracking problem of radar maneuvering target in nonlinear system model and non-Gaussian noise background, this paper puts forward one interacting multiple model (IMM) iterated extended particle filter algorithm (IMM-IEHPF). The algorithm makes use of multiple modes to model the target motion form to track any maneuvering target and each mode uses iterated extended particle filter (IEHPF) to deal with the state estimation problem of nonlinear non-Gaussian system. IEH...

  7. Extended Access List untuk Mengendalikan Trafik Jaringan

    Directory of Open Access Journals (Sweden)

    Hari Antoni Musril

    2016-12-01

    Full Text Available Keamana jaringan komputer saat ini menjadi hal penting untuk diterapkan. Banyak organisasi yang telah menjadikan teknologi informasi sebagai bahagian penting dalam menunjang aktivitasnya. Akses pengguna yang tidak dibatasi menjadi ancaman bagi sebuah organisasi, karena banyak data dan informasi penting yang tersebar dalam perangkat jaringan komputer di organisasi tersebut dapat disusupi oleh pihak yang tidak berwenang. Salah satu usaha yang dapat dilakukan adalah dengan menerapkan extended access list yang merupakan salah satu bagian dari metode access control list. Extended access list dapat menyaring lalu lintas data suatu jaringan dengan mengontrol apakah paket-paket tersebut dilewatkan atau dihentikan. Extended access list juga dapat menjamin keamanan untuk setiap komputer sehingga jalur komunikasi serta hak akses setiap komputer dapat berjalan dengan baik. Extended access list memungkinkan penyaringan berdasarkan sumber atau alamat tujuan, protokol yang dipilih, port yang digunakan, dan apakah koneksi sudah ditetapkan. Tulisan ini membahas penerapan extended access list dalam jaringan supaya dapat melakukan filter terhadap paket data yang melewati jaringan. Penerapannya menggunakan software Packet Tracer 6.1.1 untuk membuat prototipe jaringan dan mensimulasikannya. Sehingga nanti dapat diterapkan pada jaringan yang sebenarnya. List yang dibangun pada penelitian ini diterapkan untuk protokol antara lain : TCP (WWW, FTP, Telnet, SMTP, POP3, UDP (DNS, dan ICMP (Ping. Hasilnya didapatkan extended access list yang dikonfigurasi pada router dalam topologi penelitian ini mampu melakukan filter terhadap paket yang melewati jaringan. Hasil konfigurasinya sangat spesifik, sehingga penerapan hak akses permit dan deny dapat dilakukan sesuai dengan aturan dan skenario yang dirancang.

  8. Solute drag on perfect and extended dislocations

    Science.gov (United States)

    Sills, R. B.; Cai, W.

    2016-04-01

    The drag force exerted on a moving dislocation by a field of mobile solutes is studied in the steady state. The drag force is numerically calculated as a function of the dislocation velocity for both perfect and extended dislocations. The sensitivity of the non-dimensionalized force-velocity curve to the various controlling parameters is assessed, and an approximate analytical force-velocity expression is given. A non-dimensional parameter S characterizing the strength of the solute-dislocation interaction, the background solute fraction ?, and the dislocation character angle ?, are found to have the strongest influence on the force-velocity curve. Within the model considered here, a perfect screw dislocation experiences no solute drag, but an extended screw dislocation experiences a non-zero drag force that is about 10 to 30% of the drag on an extended edge dislocation. The solutes can change the spacing between the Shockley partials in both stationary and moving extended dislocations, even when the stacking fault energy remains unaltered. Under certain conditions, the solutes destabilize an extended dislocation by either collapsing it into a perfect dislocation or causing the partials to separate unboundedly. It is proposed that the latter instability may lead to the formation of large faulted areas and deformation twins in low stacking fault energy materials containing solutes, consistent with experimental observations of copper and stainless steel containing hydrogen.

  9. [Ischemic cholangiopathy induced by extended burns].

    Science.gov (United States)

    Cohen, Laurence; Angot, Emilie; Goria, Odile; Koning, Edith; François, Arnaud; Sabourin, Jean-Christophe

    2013-04-01

    Ischemic cholangiopathy is a recently described entity occurring mainly after hepatic grafts. Very few cases after intensive care unit (ICU) for extended burn injury were reported. We report the case of a 73-year-old woman consulting in an hepatology unit, for a jaundice appearing during a hospitalisation in an intensive care unit and increasing from her leaving from ICU, where she was treated for an extended burn injury. She had no pre-existing biological features of biliary disease. Biological tests were normal. Magnetic resonance imaging acquisitions of biliary tracts pointed out severe stenosing lesions of diffuse cholangiopathy concerning intrahepatic biliary tract, mainly peri-hilar. Biopsie from the liver confirmed the diagnosis, showing a biliary cirrhosis with bile infarcts. This case is the fourth case of ischemic cholangiopathy after extended burn injury, concerning a patient without a prior history of hepatic or biliary illness and appearing after hospitalisation in intensive care unit.

  10. The Extended Family and Children's Educational Success

    DEFF Research Database (Denmark)

    Jæger, Mads Meier

    2012-01-01

    Research on family background and educational success focuses almost exclusively on two generations and on parents and children. This paper argues that the extended family makes up a significant part of the total effect of family background on educational success. Empirical results based...... characteristics of grandparents and aunts and uncles have few direct effects on educational success, resources in the extended family compensate lacking resources in low-SES families, which in turn promote children’s educational success. The main conclusion is that the total effect of family background...... on the Wisconsin Longitudinal Study show that, net of family factors shared by siblings from the same immediate family, factors shared by first cousins from the same extended family account for a nontrivial part of the total variance in children’s educational success. Results also show that while socioeconomic...

  11. Rough sets: the classical and extended views

    Institute of Scientific and Technical Information of China (English)

    ZIARKO Wojciech

    2008-01-01

    The article is a comprehensive review of two major approaches to rough set theory: the classic rough setmodel introduced by Pawlak and the probabilistic approaches. The classic model is presented as a staging ground to the discussion of two varieties of the probabilistic approach, i.e. of the variable precision and Bayesian rough set models. Both of these models extend the classic model to deal with stochastic interactions while preserving the basicideas of the original rough set theory, such as set approximations, data dependencies, reducts etc. The probabilistic models are able to handle weaker data interactions than the classic model, thus extending the applicability of the rough set paradigm. The extended models are presented in considerable detail with some illustrative examples.

  12. Integration measure and extended BRST covariant quantization

    CERN Document Server

    Geyer, B; Nersessian, A P; Geyer, Bodo; Lavrov, Petr; Nersessian, Armen

    2001-01-01

    We propose an extended BRST invariant Lagrangian quantization scheme of general gauge theories based on an explicit realization of the modified triplectic algebra that was announced in our previous investigation (hep-th/0104189). The algebra includes, besides the odd operators $V^a$ appearing in the triplectic formalism, also the odd operators $U^a$ introduced within modified triplectic quantization, both of which being anti-Hamiltonian vector fields. We show that some even supersymplectic structure defined on the space of fields and antifields provides the extended BRST path integral with a well-defined integration measure. All the known Lagrangian quantization schemes based on the extended BRST symmetry are obtained by specifying the (free) parameters of that method.

  13. The geometry of branes and extended superspaces

    Energy Technology Data Exchange (ETDEWEB)

    Chryssomalakos, C. E-mail: chryss@lie3.ific.uv.es; Azcarraga, J.A. de E-mail: azcarrag@lie1.ific.uv.es; Izquierdo, J.M. E-mail: izquierd@fta.uva.es; Perez Bueno, J.C. E-mail: pbueno@lie.ific.uv.es

    2000-02-14

    We argue that a description of supersymmetric extended objects from a unified geometric point of view requires an enlargement of superspace. To this aim we study in a systematic way how superspace groups and algebras arise from Grassmann spinors when these are assumed to be the only primary entities. In the process, we recover generalized space-time superalgebras and extensions of supersymmetry found earlier. The enlargement of ordinary superspace with new parameters gives rise to extended superspace groups, on which manifestly supersymmetric actions may be constructed for various types of p-branes, including D-branes (given by Chevalley-Eilenberg cocycles) with their Born-Infeld fields. This results in a field/extended superspace democracy for superbranes: all brane fields appear as pull-backs from a suitable target superspace. Our approach also clarifies some facts concerning the origin of the central charges for the different p-branes.

  14. Extended Interneuronal Network of the Dentate Gyrus

    Directory of Open Access Journals (Sweden)

    Gergely G. Szabo

    2017-08-01

    Full Text Available Local interneurons control principal cells within individual brain areas, but anecdotal observations indicate that interneuronal axons sometimes extend beyond strict anatomical boundaries. Here, we use the case of the dentate gyrus (DG to show that boundary-crossing interneurons with cell bodies in CA3 and CA1 constitute a numerically significant and diverse population that relays patterns of activity generated within the CA regions back to granule cells. These results reveal the existence of a sophisticated retrograde GABAergic circuit that fundamentally extends the canonical interneuronal network.

  15. The extended trace identity and its application

    Institute of Scientific and Technical Information of China (English)

    Yao Yu-Qin; Chen Deng-Yuan

    2007-01-01

    The trace identity is extended to the general loop algebra.The Hamiltonian structures of the integrable systems concerning vector spectral problems and the multi-component integrable hierarchy can be worked out by Using the extended trace identity.As its application,we have obtained the Hamiltonian structures of the Yang hierarchy,the Korteweg-de-Vries (KdV) hierarchy,the multi-component Ablowitz-Kaup-Newell-Segur (M-AKNS) hierarchy,the multi-component Ablowitz-Kaup-Newell-Segur Kaup-Newell (M-AKNS-KN) hierarchy and a new multi-component integrable hierarchy separately.

  16. Dixon's extended bodies and weak gravitational waves

    CERN Document Server

    Bini, Donato; Geralico, Andrea; Ortolan, Antonello

    2009-01-01

    General relativity considers Dixon's theory as the standard theory to deal with the motion of extended bodies in a given gravitational background. We discuss here the features of the "reaction" of an extended body to the passage of a weak gravitational wave. We find that the body acquires a dipolar moment induced by its quadrupole structure. Furthermore, we derive the "world function" for the weak field limit of a gravitational wave background and use it to estimate the deviation between geodesics and the world lines of structured bodies. Measuring such deviations, due to the existence of cumulative effects, should be favorite with respect to measuring the amplitude of the gravitational wave itself.

  17. Study on an extended Boussinesq equation

    Institute of Scientific and Technical Information of China (English)

    Chen Chun-Li; Zhang Jin E; Li Yi-Shen

    2007-01-01

    An extended Boussinesq equation that models weakly nonlinear and weakly dispersive waves on a uniform layer of water is studied in this paper. The results show that the equation is not Painlevé-integrable in general. Some particular exact travelling wave solutions are obtained by using a function expansion method. An approximate solitary wave solution with physical significance is obtained by using a perturbation method. We find that the extended Boussinesq equation with a depth parameter of 1/√2 is able to match the Laitone's (1960) second order solitary wave solution of the Euler equations.

  18. Focus point supersymmetry in extended gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Ding, Ran [School of Physics, Nankai University,Tianjin 300071 (China); Li, Tianjun [State Key Laboratory of Theoretical Physics and Kavli Institute for Theoretical Physics (KITPC),Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 100190 (China); School of Physical Electronics, University of Electronic Science and Technology of China,Chengdu 610054 (China); Staub, Florian [Bethe Center for Theoretical Physics & Physikalisches Institut der Universität Bonn,Nußallee 12, 53115 Bonn (Germany); Zhu, Bin [School of Physics, Nankai University,Tianjin 300071 (China)

    2014-03-27

    We propose a small extension of the minimal gauge mediation through the combination of extended gauge mediation and conformal sequestering. We show that the focus point supersymmetry can be realized naturally, and the fine tuning is significantly reduced compared to the minimal gauge mediation and extended gauge mediation without focus point. The Higgs boson mass is around 125 GeV, the gauginos remain light, and the gluino is likely to be detected at the next run of the LHC. However, the multi-TeV squarks is out of the reach of the LHC. The numerical calculation for fine-tuning shows that this model remains natural.

  19. Market Diffusion of Extended Cycle Hormonal Contraceptives

    Directory of Open Access Journals (Sweden)

    Megen Leeds Schumacher, Pharm.D.

    2012-01-01

    Full Text Available Background: Extended cycle hormonal contraceptives (e.g. Seasonale, Seasonique when introduced in 2003 were considered a very novel approach to contraception. The idea of manipulating the menstrual cycle so that women would experience just four menstruations a year was radical and was assumed to be responsible for the slow acceptance rate among the general public.Objective: This report analyzes two different aspects of the acceptance of this unique idea in the population. The first was the level of usage of extended cycle hormonal contraceptives in the general population, which was measured by a review of sales figures over time in the United States. The second was an examination of market diffusion as it relates to consumer perceptions regarding the characteristics of these products.Methods: To determine the degree of usage of extended cycle hormonal contraceptives the yearly sales, in terms of units sold, were compared with that of other leading methods of hormonal contraception. Along with the data, survey answers were obtained from 65 women who volunteered to participate in the study. Participants were selected randomly to represent the target population to assess the level of awareness about the benefits, risks, and any other concerns regarding the use of extended cycle hormonal contraceptives.Results: The yearly sales data of units sold showed a definitive increase in the sales of extended cycle hormonal contraceptives since their release on the market. The survey results showed an overwhelming awareness in the study population about the extended regimen. However, only about half of the women in the survey group were aware of its benefits. The main concern reported was the perceived significant side effect profile.Conclusion: Though awareness about the extended cycle hormonal contraception regimen was widespread, the survey population was not well informed about the advantages and the disadvantages regarding the degree of severity of side

  20. Extending Symfony 2 web application framework

    CERN Document Server

    Armand, Sébastien

    2014-01-01

    Symfony is a high performance PHP framework for developing MVC web applications. Symfony1 allowed for ease of use but its shortcoming was the difficulty of extending it. However, this difficulty has now been eradicated by the more powerful and extensible Symfony2. Information on more advanced techniques for extending Symfony can be difficult to find, so you need one resource that contains the advanced features in a way you can understand. This tutorial offers solutions to all your Symfony extension problems. You will get to grips with all the extension points that Symfony, Twig, and Doctrine o

  1. Weak Lie symmetry and extended Lie algebra

    Energy Technology Data Exchange (ETDEWEB)

    Goenner, Hubert [Institute for Theoretical Physics, Friedrich-Hund-Platz 1, University of Goettingen, D-37077 Gottingen (Germany)

    2013-04-15

    The concept of weak Lie motion (weak Lie symmetry) is introduced. Applications given exhibit a reduction of the usual symmetry, e.g., in the case of the rotation group. In this context, a particular generalization of Lie algebras is found ('extended Lie algebras') which turns out to be an involutive distribution or a simple example for a tangent Lie algebroid. Riemannian and Lorentz metrics can be introduced on such an algebroid through an extended Cartan-Killing form. Transformation groups from non-relativistic mechanics and quantum mechanics lead to such tangent Lie algebroids and to Lorentz geometries constructed on them (1-dimensional gravitational fields).

  2. Extending the Use of Saramaccan in Suriname.

    Science.gov (United States)

    Glock, Naomi

    1983-01-01

    Advocates the use of the vernacular language, not only as an important vehicle for learning, but also as a means of enhancing the native speakers' feeling of self-worth. Presents several ways of extending the use of the vernacular, as well as the problems that can arise. (EKN)

  3. Extending and embedding the Python interpreter

    NARCIS (Netherlands)

    G. van Rossum (Guido)

    1995-01-01

    textabstractPython is an interpreted, object-oriented programming language. This document describes how to write modules in C or C++ to extend the Python interpreter with new modules. Those modules can define new functions but also new object types and their methods. The document also describes h

  4. Extending the Body for Interaction with Reality

    DEFF Research Database (Denmark)

    Feuchtner, Tiare; Müller, Jörg

    2017-01-01

    In this paper, we explore how users can control remote devices with a virtual long arm, while preserving the perception that the artificial arm is actually part of their own body. Instead of using pointing, speech, or a remote control, the users’ arm is extended in augmented reality, allowing...

  5. Macrodystrophia lipomatosa extending into the upper abdomen

    Energy Technology Data Exchange (ETDEWEB)

    Fritz, Traci R. [The University of Texas Medical Branch, Galveston, TX (United States); Swischuk, Leonard E. [The University of Texas Medical Branch, Departments of Radiology and Pediatrics, Galveston, TX (United States); The University of Texas Medical Branch, Pediatric Radiology, Galveston, TX (United States)

    2007-12-15

    We report a case of macrodystrophia lipomatosa in an infant with extension of hypertrophied fatty tissue into the abdomen and abdominal wall. The patient not only presented with typical limb abnormalities but also an abdominal mass. We reviewed the literature on the subject, and believe that our case is rare because the hypertrophied lipomatous tissue extended into the abdomen. (orig.)

  6. Extended exergy accounting applied to biodiesel production

    Energy Technology Data Exchange (ETDEWEB)

    Talens Peiro, L. [SosteniPrA (UAB-IRTA), Institute of Environmental Science and Technology (ICTA), Edifici Q-ETSE, Room QC 3101, Universitat Autonoma de Barcelona (UAB), E-08193 Bellaterra (Cerdanyola del Valles), Barcelona (Spain); Villalba Mendez, G.; Gabarrell i Durany, X. [SosteniPrA (UAB-IRTA), Institute of Environmental Science and Technology (ICTA), Edifici Q-ETSE, Room QC 3101, Universitat Autonoma de Barcelona (UAB), E-08193 Bellaterra (Cerdanyola del Valles), Barcelona (Spain); Department of Chemical Engineering, Edifici Q, Universitat Autonoma de Barcelona (UAB), E- 08193, Bellaterra (Cerdanyola del Valles), Barcelona (Spain); Sciubba, E. [Department of Mechanical and Aeronautical Engineering, University of Roma 1 ' ' La Sapienza' ' , Via Eudossiana 18, I-00184 Roma (Italy)

    2010-07-15

    When evaluating the production of renewable energies such as biofuels, it is necessary to include in the assessment the resource inputs, capital, labor investment and environmental remediation costs. Extended Exergy Accounting (EEA) is a system analysis method that calculates, on the basis of detailed mass and exergy balances, the total amount of primary exergy resources necessary to obtain a product or service. The conceptual novelty of EEA is represented by the fact that it also includes externalities (capital, labor and environmental impact) measured in homogeneous units (Joules). As an illustration of EEA, we assess and compare the production of 1 ton of biodiesel from used cooking oil (UCOME) and rapeseed crops (RME). The extended exergy ''content'' of UCOME and RME are 51.90 GJ and 77.05 GJ respectively. The production of UCOME uses 25.15 GJ less resources (materials and energy) and requires lower total investments and environmental remediation costs than that of RME. On the other hand, UCOME requires 35% more workhours. In summary, the extended exergy of UCOME is about 1.5 the extended exergy content of RME. Thus, we can conclude that biodiesel production from UCO is less resource use intensive than the production from RME. (author)

  7. OBSERVABILITY OF EXTENDED TIMED EVENT GRAPH

    Institute of Scientific and Technical Information of China (English)

    ZHUO Zhibing; CHEN Wende

    2004-01-01

    In this paper, we study some results of extended timed event graph (ETEG)by using graph theory's methods in the dioid framework. A necessary and sufficient condition for the observability of ETEG is obtained and ETEG's standard structure is also established.

  8. Extending and embedding the Python interpreter

    NARCIS (Netherlands)

    Rossum, G. van

    1995-01-01

    Python is an interpreted, object-oriented programming language. This document describes how to write modules in C or C++ to extend the Python interpreter with new modules. Those modules can define new functions but also new object types and their methods. The document also describes how to embed

  9. Extended PGA for Range Migration Algorithms

    NARCIS (Netherlands)

    Rossum, W.L. van; Otten, M.P.G.; Bree, R.J.P. van

    2006-01-01

    The Phase Gradient Autofocus (PGA) algorithm is extended to work for synthetic aperture radar (SAR) spotlight images processed with range migration (ω-k) algorithms. Several pre-processing steps are proposed for aligning the range-compressed phase-history data needed for successful autofocusing of

  10. Partial results on extending the Hopf Lemma

    CERN Document Server

    Li, YanYan

    2009-01-01

    In [1], Theorem 3, the authors proved, in one dimension, a generalization of the Hopf Lemma, and the question arose if it could be extended to higher dimensions. In this paper we present two conjectures as possible extensions, and give a very partial answer. We write this paper to call attention to the problem.

  11. Schedule-extended synchronous dataflow graphs

    NARCIS (Netherlands)

    Damavandpeyma, M.; Stuijk, S.; Basten, T.; Geilen, M.; Corporaal, H.

    2013-01-01

    Synchronous dataflow graphs (SDFGs) are used extensively to model streaming applications. An SDFG can be extended with scheduling decisions, allowing SDFG analysis to obtain properties, such as throughput or buffer sizes for the scheduled graphs. Analysis times depend strongly on the size of the

  12. Turbo Codes Extended with Outer BCH Code

    DEFF Research Database (Denmark)

    Andersen, Jakob Dahl

    1996-01-01

    The "error floor" observed in several simulations with the turbo codes is verified by calculation of an upper bound to the bit error rate for the ensemble of all interleavers. Also an easy way to calculate the weight enumerator used in this bound is presented. An extended coding scheme is proposed...

  13. Quantum field theories of extended objects

    CERN Document Server

    Friedan, Daniel

    2016-01-01

    First steps are taken in a project to construct a general class of conformal and perhaps, eventually, non-conformal quantum field theories of (n-1)-dimensional extended objects in a d=2n dimensional conformal space-time manifold M. The fields live on the spaces E of relative integral (n-1)-cycles in M -- the integral (n-1)-currents of given boundary. Each E is a complete metric space geometrically analogous to a Riemann surface $\\Sigma$. For example, if $M=S^d$, $\\Sigma = S^2$. The quantum fields on E are to be mapped to observables in a 2d CFT on $\\Sigma$. The correlation functions on E are to be given by the 2d correlation functions on $\\Sigma$. The goal is to construct a CFT of extended objects in d=2n dimensions for every 2d CFT, and eventually a non-conformal QFT of extended objects for every non-conformal 2d QFT, so that all the technology of 2d QFT can be applied to the construction and analysis of quantum field theories of extended objects. The project depends crucially on settling some mathematical q...

  14. Extending the Body for Interaction with Reality

    DEFF Research Database (Denmark)

    Feuchtner, Tiare; Müller, Jörg

    2017-01-01

    In this paper, we explore how users can control remote devices with a virtual long arm, while preserving the perception that the artificial arm is actually part of their own body. Instead of using pointing, speech, or a remote control, the users’ arm is extended in augmented reality, allowing...

  15. The Extended Mind Thesis and Mechanistic Explanations

    DEFF Research Database (Denmark)

    Fazekas, Peter

    2013-01-01

    The Extended Mind Thesis (EMT) is traditionally formulated against the bedrock of functionalism, and ongoing debates are typically bogged down with questions concerning the exact relationship between EMT and different versions of functionalism. In this paper, I offer a novel ally for EMT: the new...

  16. An extended scenario for the Schrodinger equation

    CERN Document Server

    Capak, M; Unsal, O L; Atay, S; Gonul, B

    2011-01-01

    The concept of the elegant work introduced by Levai in Ref. [5] is extended for the solutions of the Schrodinger equation with more realistic other potentials used in different disciplines of physics. The connection between the present model and the other alternative algebraic technique in the literature is discussed

  17. Experimental limits on antigravity in extended supergravity

    OpenAIRE

    1995-01-01

    The available tests of the equivalence principle constrain the mass of the Higgs-like boson appearing in extended supergravity theories. We determine the constraints imposed by the present and future high precision experiments on the antigravity fields arising from $N=2,8$ supergravity.

  18. Extended Resin Composite Restorations: Techniques and Procedures

    NARCIS (Netherlands)

    Loomans, B.A.C.; Hilton, T.

    2016-01-01

    This article gives an overview of the state of the art of different restorative treatment procedures and techniques needed for placing extended posterior resin composite restorations. Clinical aspects related to the procedure are discussed and reviewed based on the current literature, such as the

  19. Integrable extended van der Waals model

    Science.gov (United States)

    Giglio, Francesco; Landolfi, Giulio; Moro, Antonio

    2016-10-01

    Inspired by the recent developments in the study of the thermodynamics of van der Waals fluids via the theory of nonlinear conservation laws and the description of phase transitions in terms of classical (dissipative) shock waves, we propose a novel approach to the construction of multi-parameter generalisations of the van der Waals model. The theory of integrable nonlinear conservation laws still represents the inspiring framework. Starting from a macroscopic approach, a four parameter family of integrable extended van der Waals models is indeed constructed in such a way that the equation of state is a solution to an integrable nonlinear conservation law linearisable by a Cole-Hopf transformation. This family is further specified by the request that, in regime of high temperature, far from the critical region, the extended model reproduces asymptotically the standard van der Waals equation of state. We provide a detailed comparison of our extended model with two notable empirical models such as Peng-Robinson and Soave's modification of the Redlich-Kwong equations of state. We show that our extended van der Waals equation of state is compatible with both empirical models for a suitable choice of the free parameters and can be viewed as a master interpolating equation. The present approach also suggests that further generalisations can be obtained by including the class of dispersive and viscous-dispersive nonlinear conservation laws and could lead to a new type of thermodynamic phase transitions associated to nonclassical and dispersive shock waves.

  20. On energy conservation in extended magnetohydrodynamics

    Energy Technology Data Exchange (ETDEWEB)

    Kimura, Keiji [Research Institute for Mathematical Sciences, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502 (Japan); Morrison, P. J. [Department of Physics and Institute for Fusion Studies, University of Texas at Austin, Austin, Texas 78712-1060 (United States)

    2014-08-15

    A systematic study of energy conservation for extended magnetohydrodynamic models that include Hall terms and electron inertia is performed. It is observed that commonly used models do not conserve energy in the ideal limit, i.e., when viscosity and resistivity are neglected. In particular, a term in the momentum equation that is often neglected is seen to be needed for conservation of energy.

  1. Computation of unirational fields (extended abstract)

    CERN Document Server

    Gutierrez, Jaime

    2008-01-01

    In this paper we present an algorithm for computing all algebraic intermediate subfields in a separably generated unirational field extension (which in particular includes the zero characteristic case). One of the main tools is Groebner bases theory. Our algorithm also requires computing computing primitive elements and factoring over algebraic extensions. Moreover, the method can be extended to finitely generated K-algebras.

  2. Robotic extended pyelolithotomy for complete staghorn calculus.

    Science.gov (United States)

    Sotelo, Rene; Astigueta, Juan Carlos; Giedelman, Camilo; de Andrade, Robert; Carmona, Oswaldo; Ramirez, Daniel; Clavijo, Rafael

    2010-08-01

    Staghorn stones represent a therapeutic challenge to urologists. We present our experience with laparoscopic extended pyelolithotomy for treatment of staghorn and complex renal calculi in highly selected cases. This approach provides the principles of open surgery with the advantages of minimally invasive surgery. We describe our experience with robot-assisted extended pyelolithotomy for complex coralliform calculi. Since January 2007, robotic extended pyelolithotomy has been performed by transperitoneal approach in two patients with complete coralliform lithiasis (calculi average size 8 cm). One patient had history of percutaneous nephrolithotomy. Demographic and operative data were collected. All procedures were technically successful without need for open conversion. Mean estimated blood loss was 175 ml (range 50-300 ml), and mean operative time was 150 min (range 120-150 min). A perinephric drain was employed in one patient with duration of 5 days. Postoperative imaging confirmed complete stone clearance. Robotic extended pyelolithotomy is a feasible and reproducible procedure for removal of complete and partial staghorn calculi in selected patients with complex nephrolithiasis. This approach might limit the role of open surgery for these calculi, but further publications with more cases are necessary to further define its utility.

  3. An Extended Analysis of Requirements Traceability Model

    Institute of Scientific and Technical Information of China (English)

    Jiang Dandong(蒋丹东); Zhang Shensheng; Chen Lu

    2004-01-01

    A new extended meta model of traceability is presented. Then, a formalized fine-grained model of traceability is described. Some major issues about this model, including trace units, requirements and relations within the model, are further analyzed. Finally, a case study that comes from a key project of 863 Program is given.

  4. Extending and embedding the Python interpreter

    NARCIS (Netherlands)

    G. van Rossum (Guido)

    1995-01-01

    textabstractPython is an interpreted, object-oriented programming language. This document describes how to write modules in C or C++ to extend the Python interpreter with new modules. Those modules can define new functions but also new object types and their methods. The document also describes

  5. Extending School Hours: A Capital Idea.

    Science.gov (United States)

    Jones, John Hodge

    1995-01-01

    The Murfreesboro, Tennessee, City Schools have doubled the amount of time schools are open without raising taxes. Instead of going home to empty houses, kids participate in an extended school program offering over 100 courses in recreation, academics, arts, and life skills. Parents and organizations foot the costs; no child is excluded for…

  6. Spontaneous Breakup of Extended Monodisperse Polymer Melts

    DEFF Research Database (Denmark)

    Rasmussen, Henrik K.; Yu, Kaijia

    2011-01-01

    We apply continuum mechanical based, numerical modeling to study the dynamics of extended monodisperse polymer melts during the relaxation. The computations are within the ideas of the microstructural ‘‘interchain pressure’’ theory. The computations show a delayed necking resulting in a rupture...

  7. An Extended Chiral SU(3) Quark Model

    Institute of Scientific and Technical Information of China (English)

    ZHANG Zong-Ye; YU You-Wen; WANG Ping; DAI Lian-Rong

    2003-01-01

    The chiral SU(3) quark model is extended by including the vector meson exchanges to describe the short range interactions. The phase shifts of NN scattering are studied in this model. Compared with the results of the chiral SU(3) quark model in which only the pseudo-scalar and scalar chiralfields are considered, the phase shifts of 1 So wave are obviously improved.

  8. Q-Method Extended Kalman Filter

    Science.gov (United States)

    Zanetti, Renato; Ainscough, Thomas; Christian, John; Spanos, Pol D.

    2012-01-01

    A new algorithm is proposed that smoothly integrates non-linear estimation of the attitude quaternion using Davenport s q-method and estimation of non-attitude states through an extended Kalman filter. The new method is compared to a similar existing algorithm showing its similarities and differences. The validity of the proposed approach is confirmed through numerical simulations.

  9. Simulation and analysis of shared extended mind

    NARCIS (Netherlands)

    Bosse, T.; Jonker, C.M.; Schut, M.C.; Treur, J.

    2008-01-01

    Some types of animals exploit patterns created in the environment as external mental states, thus obtaining an extension of their mind. In the case of social animals the creation and exploitation of such patterns can be shared, which supports a form of shared extended mind or collective intelligence

  10. Exploring Social Structures in Extended Team Model

    DEFF Research Database (Denmark)

    Zahedi, Mansooreh; Ali Babar, Muhammad

    2013-01-01

    Extended Team Model (ETM) as a type of offshore outsourcing is increasingly becoming popular mode of Global Software Development (GSD). There is little knowledge about the social structures in ETM and their impact on collaboration. Within a large interdisciplinary project to develop the next gene...

  11. The Extended Family and Children's Educational Success

    Science.gov (United States)

    Jaeger, Mads Meier

    2012-01-01

    Research on family background and educational success focuses almost exclusively on two generations: parents and children. This study argues that the extended family contributes significantly to the total effect of family background on educational success. Analyses using the Wisconsin Longitudinal Study show that, net of family factors shared by…

  12. [Combined hormonal contraception in cycles artificially extended].

    Science.gov (United States)

    Bustillos-Alamilla, Edgardo; Zepeda-Zaragoza, J; Hernández-Ruiz, M A; Briones-Landa, Carlos Humberto

    2010-01-01

    To compare the bleeding patterns, satisfaction and tolerability of 3 different contraceptive in an extended regimens in the service of Family Planning of the North Central Hospital of PEMEX. Healthy, adult women with desire of contraception for one year (N 120) were randomly assigned to receive oral contraceptive drospirenone/ethinyl E2 (group1), the norelgestromin/ethinyl E2 transdermal patch (group 2) and vaginal ring etonogestrel/ ethinyl E2 (group 3) in an extended regimen (42 consecutive days, 1 hormone-free week). Study assessments were conducted at scheduled visits at the time of initial screening, at baseline after 1, 3, 6, and 12 months. Subjects recorded menstrual associated symptoms bleeding data and completed satisfaction questionnaires. Subjects and investigators provided overall assessments of the regimens. Extended use of 3 different contraceptive resulted in fewer bleeding days in every group (66.6%, 55% and 58.3% P 0.0024), and less mastalgia and menstrual pain. Subjects were highly satisfied with three regimens (93.3%, 96.6% and 91.6% P 0.00421). Although not mayor adverse events were reported with this regimen, there was an increase in spotting days; it decreased with each successive cycle of therapy. Efficacy and safety were similar to those reported for traditional cycle. Extended-contraceptive regimen delays menses and reduces bleeding, a profile that may be preferred by women who seek flexibility with their contraceptive method.

  13. From ergodicity to extended phase diagrams.

    Science.gov (United States)

    Woodley, Scott M; Sokol, Alexey A

    2012-04-16

    Structure prediction of stable and metastable phases is put on equal footing for the first time, with a solid thermodynamical background. How to estimate the lifetime of metastable phases is demonstrated by recent groundbreaking work of Jansen, Pentin, and Schön. At the heart lies the exploration of the Gibbs free-energy landscapes and the extended phase diagrams for complex systems.

  14. Prototype for Extended XDB Using Wiki

    Directory of Open Access Journals (Sweden)

    Wook-Sung Yoo

    2012-11-01

    Full Text Available This paper describes a prototype of extended XDB. XDB is an open-source and extensible database architecture developed by National Aeronautics and Space Administration (NASA to provide integration of heterogeneous and distributed information resources for scientific and engineering applications. XDB enables an unlimited number of desktops and distributed information sources to be linked seamlessly and efficiently into an information grid using Data Access and Retrieval Composition (DARC protocol whichprovides a contextual search and retrieval capability useful for lightweight web applications. This paper shows the usage of XDB on common data management in the enterprise without burdening users and application developers with unnecessary complexity and formal schemas. Supported by NASA Ames Research Center through NASA Exploration System Mission Directorate (ESMD Higher Education grant, a project team at Fairfield University extended this concept and developed an extended XDB protocol and a prototype providing text-searches for Wiki. The technical specification of the protocol was posted to Source Forge (sourceforge.net and a prototype providing text-searches for Wiki was developed. The prototype was created for 16 tags of the MediaWiki dialect. As part of future works, the prototype will be further extended to the complete Wiki markups and other dialects of Wiki.

  15. Symmetric cryptographic protocols for extended millionaires' problem

    Institute of Scientific and Technical Information of China (English)

    LI ShunDong; WANG DaoShun; DAI YiQi

    2009-01-01

    Yao's millionaires' problem is a fundamental problem in secure multiparty computation, and its solutions have become building blocks of many secure multiparty computation solutions. Unfortunately,most protocols for millionaires' problem are constructed based on public cryptography, and thus are inefficient. Furthermore, all protocols are designed to solve the basic millionaires' problem, that is,to privately determine which of two natural numbers is greater. If the numbers are real, existing solutions do not directly work. These features limit the extensive application of the existing protocols. This study introduces and refines the first symmetric cryptographic protocol for the basic millionaires' problem, and then extends the symmetric cryptographic protocol to privately determining which of two real numbers is greater, which are called the extended millionaires' problem, and proposes corresponding Constructed based on symmetric cryptography, these protocols are very efficient.

  16. Averaged Extended Tree Augmented Naive Classifier

    Directory of Open Access Journals (Sweden)

    Aaron Meehan

    2015-07-01

    Full Text Available This work presents a new general purpose classifier named Averaged Extended Tree Augmented Naive Bayes (AETAN, which is based on combining the advantageous characteristics of Extended Tree Augmented Naive Bayes (ETAN and Averaged One-Dependence Estimator (AODE classifiers. We describe the main properties of the approach and algorithms for learning it, along with an analysis of its computational time complexity. Empirical results with numerous data sets indicate that the new approach is superior to ETAN and AODE in terms of both zero-one classification accuracy and log loss. It also compares favourably against weighted AODE and hidden Naive Bayes. The learning phase of the new approach is slower than that of its competitors, while the time complexity for the testing phase is similar. Such characteristics suggest that the new classifier is ideal in scenarios where online learning is not required.

  17. Extending STL with Efficient Data Structures

    Institute of Scientific and Technical Information of China (English)

    吴虹; 聂旭民

    1998-01-01

    The Standard Template Library(STL)is a C++ library that provides a set of well structured generic C++ components that work together.Based on its well-structured design,STL can be extended by adding new generic algorithms or new generic containers.In this paper,the work on extending STY by adding several efficient container classes for threaded binary search trees is discussed.It is shown that the addition of threads significantly increases the efficiency of some important access operations on the binary tree containers.In general,significant gain in efficiency can be expected in situations where the data structures ar relatively stable,that is,the data structures are looked up often but modified little.

  18. Thermodynamic Products in the Extended Phase Space

    CERN Document Server

    Pradhan, Parthapratim

    2016-01-01

    We have examined the thermodynamic properties of spherically symmetric charged-AdS black hole, charged AdS BH surrounded by quintessence and charged AdS BH in $f(R)$ gravity in the extended phase-space. Where the cosmological constant should be treated as thermodynamic pressure and its conjugate parameter as thermodynamic volume. Then they should behave as a analog of Van der Waal like systems. In the extended phase space we have calculated the \\emph{entropy product} and \\emph{thermodynamic volume product} of all horizons. The mass(or enthalpy) independent nature of the said products signals they are "universal" quantities. Various types of pictorial diagram of the specific heat is given. The divergence of the specific heat indicates that the second order phase transition occurs under certain condition.

  19. Quantum gravity kinematics from extended TQFTs

    CERN Document Server

    Dittrich, Bianca

    2016-01-01

    We show how extended topological quantum field theories (TQFTs) can be used to obtain a kinematical setup for quantum gravity, i.e. a kinematical Hilbert space together with a representation of the observable algebra including operators of quantum geometry. In particular, we consider the holonomy-flux algebra of (2+1)-dimensional Euclidean loop quantum gravity, and construct a new representation of this algebra that incorporates a positive cosmological constant. The vacuum state underlying our representation is defined by the Turaev-Viro TQFT. We therefore construct here a generalization, or more precisely a quantum deformation at root of unity, of the previously-introduced SU(2) BF representation. The extended Turaev-Viro TQFT provides a description of the excitations on top of the vacuum, which are essential to allow for a representation of the holonomies and fluxes. These excitations agree with the ones induced by massive and spinning particles, and therefore the framework presented here allows automatical...

  20. Beyond six parameters: extending $\\Lambda$CDM

    CERN Document Server

    Di Valentino, Eleonora; Silk, Joseph

    2015-01-01

    Cosmological constraints are usually derived under the assumption of a $6$ parameters $\\Lambda$-CDM theoretical framework or simple one-parameter extensions. In this paper we present, for the first time, cosmological constraints in a significantly extended scenario, varying up to $12$ cosmological parameters simultaneously, including the sum of neutrino masses, the neutrino effective number, the dark energy equation of state, the gravitational waves background and the running of the spectral index of primordial perturbations. Using the latest Planck 2015 data release (with polarization) we found no significant indication for extensions to the standard $\\Lambda$-CDM scenario, with the notable exception of the angular power spectrum lensing amplitude, $A_{\\rm lens}$ that is larger than the expected value at more than two standard deviations even when combining the Planck data with BAO and supernovae type Ia external datasets. In our extended cosmological framework, we find that a combined Planck+BAO analysis co...

  1. Extended external hemipelvectomy for sigmoid adenocarcinoma treatment.

    Science.gov (United States)

    Reis, Rodrigo Ribas Dias dos; Schiavinatto, Luciane; Telles, Mário Luis; Moreira, Adriana Eliza Brasil; Mainardi, João Paulo Aguiar Jordão

    2010-10-01

    Surgery is the most effective way of treating a locally advanced colorectal carcinoma and an extended en bloc resection is necessary to achieve the best overall survival rate. In this rare case, a multi-visceral resection was performed along with the entire lower left limb and left iliac bone for a sigmoid carcinoma. The T4N0M0 (B3) tumor involved the left iliac vessels, left pelvic wall, small bowel and both rectus muscles, besides presenting with a skin fistula. A Hartmann colostomy was also performed. Chemotherapy was interrupted because of toxicity. The patient is free of disease after 38 months. There are very few cases that describe an extended hemipelvectomy as part of a colorectal carcinoma treatment.

  2. An extended retinotopic map of mouse cortex

    Science.gov (United States)

    Zhuang, Jun; Ng, Lydia; Williams, Derric; Valley, Matthew; Li, Yang; Garrett, Marina; Waters, Jack

    2017-01-01

    Visual perception and behavior are mediated by cortical areas that have been distinguished using architectonic and retinotopic criteria. We employed fluorescence imaging and GCaMP6 reporter mice to generate retinotopic maps, revealing additional regions of retinotopic organization that extend into barrel and retrosplenial cortices. Aligning retinotopic maps to architectonic borders, we found a mismatch in border location, indicating that architectonic borders are not aligned with the retinotopic transition at the vertical meridian. We also assessed the representation of visual space within each region, finding that four visual areas bordering V1 (LM, P, PM and RL) display complementary representations, with overlap primarily at the central hemifield. Our results extend our understanding of the organization of mouse cortex to include up to 16 distinct retinotopically organized regions. DOI: http://dx.doi.org/10.7554/eLife.18372.001 PMID:28059700

  3. Thermalization and pseudolocality in extended quantum systems

    CERN Document Server

    Doyon, Benjamin

    2015-01-01

    Recently, it was understood that extended concepts of locality played important roles in the study of extended quantum systems out of equilibrium, in particular in so-called generalized Gibbs ensembles. In this paper, we rigorously study pseudolocal charges and their involvement in time evolutions and in the thermalization process of arbitrary states with strong enough clustering properties. We show that the densities of pseudolocal charges form a Hilbert space, with inner product determined by response functions. Using this, we define the family of pseudolocal states: clustering states connected to the infinite-temperature state by paths whose tangents are actions of pseudolocal charges. This family includes thermal Gibbs states, as well as (a precise definition of) generalized Gibbs ensembles. We prove that the family of pseudolocal states is preserved by finite time evolution, and that, under certain conditions, the stationary state emerging at infinite time is a generalized Gibbs ensemble with respect to ...

  4. Quantum Measurement and Extended Feynman Path Integral

    Institute of Scientific and Technical Information of China (English)

    文伟; 白彦魁

    2012-01-01

    Quantum measurement problem has existed many years and inspired a large of literature in both physics and philosophy, but there is still no conclusion and consensus on it. We show it can be subsumed into the quantum theory if we extend the Feynman path integral by considering the relativistic effect of Feynman paths. According to this extended theory, we deduce not only the Klein-Gordon equation, but also the wave-function-collapse equation. It is shown that the stochastic and instantaneous collapse of the quantum measurement is due to the "potential noise" of the apparatus or environment and "inner correlation" of wave function respectively. Therefore, the definite-status of the macroscopic matter is due to itself and this does not disobey the quantum mechanics. This work will give a new recognition for the measurement problem.

  5. Assessing change with the extended logistic model.

    Science.gov (United States)

    Cristante, Francesca; Robusto, Egidio

    2007-11-01

    The purpose of this article is to define a method for the assessment of change. A reinterpretation of the extended logistic model is proposed. The extended logistic model for the assessment of change (ELMAC) allows the definition of a time parameter which is supposed to identify whether change occurs during a period of time, given a specific event or phenomenon. The assessment of a trend of change through time, on the basis of the time parameter which is estimated at different successive occasions during a period of time, is also considered. In addition, a dispersion parameter is calculated which identifies whether change is consistent at each time point. The issue of independence is taken into account both in relation to the time parameter and the dispersion parameter. An application of the ELMAC in a learning process is presented. The interpretation of the model parameters and the model fit statistics is consistent with expectations.

  6. GQL: Extending XQuery to Query GML Documents

    Institute of Scientific and Technical Information of China (English)

    GUAN Jihong; ZHU Fubao; ZHOU Jiaogen; NIU Liping

    2006-01-01

    GML is becoming the de facto standard for electronic data exchange among the applications of Web and distributed geographic information systems. However, the conventional query languages (e.g. SQL and its extended versions) are not suitable for direct querying and updating of GML documents. Even the effective approaches working well with XML could not guarantee good results when applied to GML documents. Although XQuery is a powerful standard query language for XML, it is not proposed for querying spatial features, which constitute the most important components in GML documents. We propose GQL, a query language specification to support spatial queries over GML documents by extending XQuery. The data model, algebra, and formal semantics as well as various spatial functions and operations of GQL are presented in detail.

  7. Korea Peninsula Landscape Extends from Continent End

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ The Korea peninsula extends southward from the eastern end of the Asian continent. It is divided just slightly north of the 38th parallel. The peninsula is roughly 1020 km(612 miles) long and 175 km(105 miles) wide at its narrowest point. Korean people are big sports fans. During the past 20 years, Korea has hosted many international sports events including the 1988 Seoul Olympic Games and the 2002 FIFA World Cup, and has achieved excellent results in various sports competitions.

  8. FRW Cosmology with the Extended Chaplygin Gas

    Directory of Open Access Journals (Sweden)

    B. Pourhassan

    2014-01-01

    Full Text Available We propose extended Chaplygin gas equation of state for which it recovers barotropic fluid with quadratic equation of state. We use numerical method to investigate the behavior of some cosmological parameters such as scale factor, Hubble expansion parameter, energy density, and deceleration parameter. We also discuss the resulting effective equation of state parameter. Using density perturbations we investigate the stability of the theory.

  9. Condensed Extended Hyper-Wiener Index

    Institute of Scientific and Technical Information of China (English)

    LI Xin-Hua; Abraham F. Jalbout; JI Zhi

    2008-01-01

    According to the definitions of molecular connectivity and hyper-Wiener index, a novel set of hyper-Wiener indexes (Dn, mDn) were defined and named as condensed extended hyper-Wiener index, the potential usefulness of which in QSAR/QSPR is evaluated by its correlation with a number of C3-C8 alkanes as well as by a favorable comparison with models based on molecular connectivity index and overall Wiener index.

  10. Lepton flavor violation in an extended MSSM

    CERN Document Server

    Espinosa-Castañeda, R.; Gómez-Bock, M.; Mondragón, M.

    2016-01-01

    In this work we explore a lepton flavor violation effect induced at one loop for a flavor structure in an extended minimal standard supersymmetric model, considering an ansatz for the trilinear term. In particular we find a finite expression which will show the impact of this phenomena in the $h\\to \\mu \\tau$ decay, produced by a mixing in the trilinear coupling of the soft supersymmetric Lagrangian.

  11. Extended degree functions and monomial modules

    OpenAIRE

    2004-01-01

    The arithmetic degree, the smallest extended degree, and the homological degree are invariants that have been proposed as alternatives of the degree of a module if this module is not Cohen-Macaulay. We compare these degree functions and study their behavior when passing to the generic initial or the lexicographic submodule. This leads to various bounds and to counterexamples to a conjecture of Gunston and Vasconcelos, respectively. Particular attention is given to the class of sequentially Co...

  12. Extending Science lessons with Virtual Reality

    OpenAIRE

    Minocha, Shailey; Tudor, Ana-Despina; Tilling, Steve; Needham, Richard

    2016-01-01

    The Open University, Field Studies Council and Association for Science Education are conducting research into the use of Google Expeditions and other virtual reality tools to a) augment and extend field work experiences; and b) as an additional tool in the classrooms along with resources such as videos, photographs. \\ud \\ud The following aspects were discussed in this workshop:\\ud \\ud Does the virtual reality technology improve student engagement, and what are the implications for teachers?\\u...

  13. From self-organized to extended criticality

    Directory of Open Access Journals (Sweden)

    Elisa eLovecchio

    2012-04-01

    Full Text Available We address the issue of criticality that is attracting the attention of an increasing number of neurophysiologists. Our main purpose is to establish the specific nature of some dynamical processes that although physically different, are usually termed as "critical", and we focus on those characterized by the cooperative interaction of many units. We notice that the term "criticality" has been adopted to denote both noise-induced phase transitions and Self-Organized Criticality (SOC with no clear connection with the traditional phase transitions, namely the transformation of a thermodynamic system from one state of matter to another. We notice the recent attractive proposal of extended criticality advocated by Bailly and Longo, which is realized through a wide set of critical points rather than emerging as a singularity from a unique value of the control parameter. We study a set of cooperatively firing neurons and we show that for an extended set of interaction couplings the system exhibits a form of temporal complexity similar to that emerging at criticality from ordinary phase transitions. This extended criticality regime is characterized by three main properties: i In the ideal limiting case of infinitely large time period, temporal complexity corresponds to Mittag-Leffler complexity; ii For large values of the interaction coupling the periodic nature of the process becomes predominant while maintaining to some extent, in the intermediate time asymptotic region, the signature of complexity; iii Focusing our attention on firing neuron avalanches, we find two of the popular SOC properties, namely the power indexes 2 and 1.5 respectively for time length and for the intensity of the avalanches. We derive the conclusion that SOC emerges from extended criticality, thereby explaining the experimental observation of Plenz and Beggs: avalanches occur in time with surprisingly regularity, in apparent conflict with he temporal complexity of physical

  14. Extended Deterministic Mean-Field Games

    KAUST Repository

    Gomes, Diogo A.

    2016-04-21

    In this paper, we consider mean-field games where the interaction of each player with the mean field takes into account not only the states of the players but also their collective behavior. To do so, we develop a random variable framework that is particularly convenient for these problems. We prove an existence result for extended mean-field games and establish uniqueness conditions. In the last section, we consider the Master Equation and discuss properties of its solutions.

  15. Changing Requirements of Assurance and Extended Deterrence

    Science.gov (United States)

    2010-07-01

    Larsen, eds., Weapons of Mass Destruction: An Encyclopedia of Worldwide Policy, Technology, and History, Vol. II (Santa Barbara, CA: ABC - CLIO, 2005...Appendix A Abbreviations ABM Anti-Ballistic Missile ABMT Anti-Ballistic Missile Treaty AEIO Atomic Energy Organization of Iran AKP Justice and... ABC -CLIO, 2005. Thranert, Oliver. “NATO, Missile Defence and Extended Deterrence.” Survival 51:6 (December2009-January 2010): 63–75. Wheeler

  16. The Extended Fock Basis of Clifford Algebra

    CERN Document Server

    Budinich, Marco

    2010-01-01

    We investigate the properties of the Extended Fock Basis (EFB) of Clifford algebras introduced in [1]. We show that a Clifford algebra can be seen as a direct sum of multiple spinor subspaces that are characterized as being left eigenvectors of $\\Gamma$. We also show that a simple spinor, expressed in Fock basis, can have a maximum number of non zero coordinates that equals the size of the maximal totally null plane (with the notable exception of vectorial spaces with 6 dimensions).

  17. The Extended Fock Basis of Clifford Algebra

    OpenAIRE

    2010-01-01

    We investigate the properties of the Extended Fock Basis (EFB) of Clifford algebras introduced in [1]. We show that a Clifford algebra can be seen as a direct sum of multiple spinor subspaces that are characterized as being left eigenvectors of \\Gamma. We also show that a simple spinor, expressed in Fock basis, can have a maximum number of non zero coordinates that equals the size of the maximal totally null plane (with the notable exception of vectorial spaces with 6 dimensions).

  18. Knowledge Management System Design using Extended Gaia

    CERN Document Server

    Jain, Pooja; 10.5121/ijcnc.2011.3109

    2011-01-01

    An efficient Learning resource centre can be achieved with the help of a network of collaborating, coordinating and communicating software agents. Agent-oriented techniques represent an exciting new means of analysing, designing and building complex software systems. The designing of the interacting agents is done with the help of Gaia, extended for the multiagent systems. Gaia is a methodology for agent-oriented analysis and design proposed by M. Wooldridge [9].

  19. Knowledge Management System Design Using Extended Gaia

    Directory of Open Access Journals (Sweden)

    Pooja Jain

    2011-01-01

    Full Text Available An efficient Learning resource centre can be achieved with the help of a network of collaborating,coordinating and communicating software agents. Agent-oriented techniques represent an exciting newmeans of analysing, designing and building complex software systems. The designing of the interactingagents is done with the help of Gaia, extended for the multiagent systems. Gaia is a methodology foragent-oriented analysis and design proposed by M. Wooldridge [9].

  20. An Extended Colored Zee-Babu Model

    CERN Document Server

    Nomura, Takaaki

    2016-01-01

    We study the extended colored Zee-Babu model introducing a vector-like quark and singlet scalar. The active neutrino mass matrix and muon anomalous magnetic moment are analyzed, which can be fitted to experimental data satisfying the constraints from flavor changing neutral current. Then we discuss signature of our model via vector-like quark production. In addition, the diphoton excess can be explained with the contribution from vector-like quark

  1. Extending DUNE: The dune-xt modules

    OpenAIRE

    Leibner, Tobias; Milk, René; Schindler, Felix

    2016-01-01

    We present our effort to extend and complement the core modules of the Distributed and Unified Numerics Environment DUNE (http://dune-project.org) by a well tested and structured collection of utilities and concepts. We describe key elements of our four modules dune-xt-common, dune-xt-grid, dune-xt-la and dune-xt-functions, which aim at further enabling the programming of generic algorithms within DUNE as well as adding an extra layer of usability and convenience.

  2. Rugged, Tunable Extended-Cavity Diode Laser

    Science.gov (United States)

    Moore, Donald; Brinza, David; Seidel, David; Klipstein, William; Choi, Dong Ho; Le, Lam; Zhang, Guangzhi; Iniguez, Roberto; Tang, Wade

    2007-01-01

    A rugged, tunable extended-cavity diode laser (ECDL) has been developed to satisfy stringent requirements for frequency stability, notably including low sensitivity to vibration. This laser is designed specifically for use in an atomic-clock experiment to be performed aboard the International Space Station (ISS). Lasers of similar design would be suitable for use in terrestrial laboratories engaged in atomic-clock and atomic-physics research.

  3. The renewable tradition (extended play remix

    Directory of Open Access Journals (Sweden)

    Mark Amerika

    2009-12-01

    Full Text Available Drawing from a network of appropriation artists, process philosophers, performance poets, pla(ygiarists, and other remix provocateurs, this experimental artist essay investigates the act of remixologically inhabiting the body language of others and, in the process, devises an aesthetic theory that situates the contemporary artist as a "postproduction medium" whose methodologies further extend avant-garde practice into the 21st century.

  4. Mobile Agent PLM Architecture for extended enterprise

    Directory of Open Access Journals (Sweden)

    Abdelhak Boulaalam

    2011-01-01

    Full Text Available Nowadays manufacturers are under increased pressure to have an add value for their products to struggle the low-cost production in emerging countries. Distributed control and Intelligent Product are a new and exciting opportunity to build more effective process networks for a wide range of applications in logistics and product development, Radio Frequency Identification is applied increasingly; this technology applied in conjunction with the Mobile Agent system can bring more values in managing and control the lifecycle of products by optimizing the three essential factors: cost, quality and deadline for the survival of a company in the competitive manufacturing world. In this paper we propose Mobile Agent PLM Architecture for extended enterprise, based on Mobile Agent and RFID or, more generally, Product Embedded Information Devices (PEID, for tracking and managing the information of the whole product lifecycle in the extended enterprise, and to satisfy new requirements for increased integrability, traceability, adaptability, extendibility, and closed-loop PLM. Mobile Agents are suitable for tracking information in distributing environment and the mobility aspect, at any time and any place. This paper proposes a first architecture based on these technologies.

  5. Inviscid Analysis of Extended Formation Flight

    Science.gov (United States)

    Kless, James; Aftosmis, Michael J.; Ning, Simeon Andrew; Nemec, Marian

    2012-01-01

    Flying airplanes in extended formations, with separation distances of tens of wingspans, significantly improves safety while maintaining most of the fuel savings achieved in close formations. The present study investigates the impact of roll trim and compressibility at fixed lift coefficient on the benefits of extended formation flight. An Euler solver with adjoint-based mesh refinement combined with a wake propagation model is used to analyze a two-body echelon formation at a separation distance of 30 spans. Two geometries are examined: a simple wing and a wing-body geometry. Energy savings, quantified by both formation drag fraction and span efficiency factor, are investigated at subsonic and transonic speeds for a matrix of vortex locations. The results show that at fixed lift and trimmed for roll, the optimal location of vortex impingement is about 10% inboard of the trailing airplane s wing-tip. Interestingly, early results show the variation in drag fraction reduction is small in the neighborhood of the optimal position. Over 90% of energy benefits can be obtained with a 5% variation in transverse and 10% variation in crossflow directions. Early results suggest control surface deflections required to achieve trim reduce the benefits of formation flight by 3-5% at subsonic speeds. The final paper will include transonic effects and trim on extended formation flight drag benefits.

  6. Extended-reach wells tap outlying reserves

    Energy Technology Data Exchange (ETDEWEB)

    Nazzal, G. (Eastman Teleco, Houston, TX (United States))

    1993-03-01

    Extended-reach drilling (ERD) is being used to exploit fields and reserves that are located far from existing platforms. Effective wellbore placement from fewer platforms can reduce development costs, maximize production and increase reserve recovery. Six wells drilled offshore in the US, North Sea and Australia illustrate how to get the most economic benefit from available infrastructure. These wells are divided into three categories by depth (shallow, medium and deep). Vertical depth of these wells range from 963 to 12,791 ft TVD and displacements range from 4,871 to 23,917 ft. Important factors for successful extended-reach drilling included: careful, comprehensive pre-planning; adequate cuttings removal in all sections; hole stability in long, exposed intervals; torque and drag modeling of drilling BHAs, casing and liners; buoyancy-assisted casing techniques where appropriate; critical modifications to drilling rig and top drive, for medium and deep ERD; modified power swivels for shallow operations; drill pipe rubbers or other casing protection during extended periods of drill string rotation; heavy-wall casting across anticipated high-wear areas; survey accuracy and frequency; sound drilling practices and creativity to accomplish goals and objectives. This paper reviews the case history of these sites and records planning and design procedures.

  7. Why an extended evolutionary synthesis is necessary.

    Science.gov (United States)

    Müller, Gerd B

    2017-10-06

    Since the last major theoretical integration in evolutionary biology-the modern synthesis (MS) of the 1940s-the biosciences have made significant advances. The rise of molecular biology and evolutionary developmental biology, the recognition of ecological development, niche construction and multiple inheritance systems, the '-omics' revolution and the science of systems biology, among other developments, have provided a wealth of new knowledge about the factors responsible for evolutionary change. Some of these results are in agreement with the standard theory and others reveal different properties of the evolutionary process. A renewed and extended theoretical synthesis, advocated by several authors in this issue, aims to unite pertinent concepts that emerge from the novel fields with elements of the standard theory. The resulting theoretical framework differs from the latter in its core logic and predictive capacities. Whereas the MS theory and its various amendments concentrate on genetic and adaptive variation in populations, the extended framework emphasizes the role of constructive processes, ecological interactions and systems dynamics in the evolution of organismal complexity as well as its social and cultural conditions. Single-level and unilinear causation is replaced by multilevel and reciprocal causation. Among other consequences, the extended framework overcomes many of the limitations of traditional gene-centric explanation and entails a revised understanding of the role of natural selection in the evolutionary process. All these features stimulate research into new areas of evolutionary biology.

  8. Extended pie menus for immersive virtual environments.

    Science.gov (United States)

    Gebhardt, Sascha; Pick, Sebastian; Leithold, Franziska; Hentschel, Bernd; Kuhlen, Torsten

    2013-04-01

    Pie menus are a well-known technique for interacting with 2D environments and so far a large body of research documents their usage and optimizations. Yet, comparatively little research has been done on the usability of pie menus in immersive virtual environments (IVEs). In this paper we reduce this gap by presenting an implementation and evaluation of an extended hierarchical pie menu system for IVEs that can be operated with a six-degrees-of-freedom input device. Following an iterative development process, we first developed and evaluated a basic hierarchical pie menu system. To better understand how pie menus should be operated in IVEs, we tested this system in a pilot user study with 24 participants and focus on item selection. Regarding the results of the study, the system was tweaked and elements like check boxes, sliders, and color map editors were added to provide extended functionality. An expert review with five experts was performed with the extended pie menus being integrated into an existing VR application to identify potential design issues. Overall results indicated high performance and efficient design.

  9. Origin of density fluctuations in extended inflation

    Science.gov (United States)

    Kolb, Edward W.; Salopek, David S.; Turner, Michael S.

    1990-01-01

    The density fluctuations (both curvature and isocurvature) that arise due to quantum fluctuations in a simple model of extended inflation based upon the Jordan-Brans-Dicke theory are calculated. Curvature fluctuations arise due to quantum fluctuations in the Brans-Dicke field, in general have a nonscale-invariant spectrum, and can have an amplitude that is cosmologically acceptable and interesting without having to tune any coupling constant to a very small value. The density perturbations that arise due to the inflation field are subdominant. If there are other massless fields in the theory, e.g., an axion or an ilion, then isocurvature fluctuations arise in these fields too. Production of gravitational waves and the massless particles associated with excitations of the Brans-Dicke field are also discussed. Several attempts at more realistic models of extended inflation are also analyzed. The importance of the Einstein conformal frame in calculating curvature fluctuations is emphasized. When viewed in this frame, extended inflation closely resembles slow-rollover inflation with an exponential potential and the usual formula for the amplitude of curvature perturbations applies.

  10. Dark energy and extended dark matter halos

    Science.gov (United States)

    Chernin, A. D.; Teerikorpi, P.; Valtonen, M. J.; Dolgachev, V. P.; Domozhilova, L. M.; Byrd, G. G.

    2012-03-01

    The cosmological mean matter (dark and baryonic) density measured in the units of the critical density is Ωm = 0.27. Independently, the local mean density is estimated to be Ωloc = 0.08-0.23 from recent data on galaxy groups at redshifts up to z = 0.01-0.03 (as published by Crook et al. 2007, ApJ, 655, 790 and Makarov & Karachentsev 2011, MNRAS, 412, 2498). If the lower values of Ωloc are reliable, as Makarov & Karachentsev and some other observers prefer, does this mean that the Local Universe of 100-300 Mpc across is an underdensity in the cosmic matter distribution? Or could it nevertheless be representative of the mean cosmic density or even be an overdensity due to the Local Supercluster therein. We focus on dark matter halos of groups of galaxies and check how much dark mass the invisible outer layers of the halos are able to host. The outer layers are usually devoid of bright galaxies and cannot be seen at large distances. The key factor which bounds the size of an isolated halo is the local antigravity produced by the omnipresent background of dark energy. A gravitationally bound halo does not extend beyond the zero-gravity surface where the gravity of matter and the antigravity of dark energy balance, thus defining a natural upper size of a system. We use our theory of local dynamical effects of dark energy to estimate the maximal sizes and masses of the extended dark halos. Using data from three recent catalogs of galaxy groups, we show that the calculated mass bounds conform with the assumption that a significant amount of dark matter is located in the invisible outer parts of the extended halos, sufficient to fill the gap between the observed and expected local matter density. Nearby groups of galaxies and the Virgo cluster have dark halos which seem to extend up to their zero-gravity surfaces. If the extended halo is a common feature of gravitationally bound systems on scales of galaxy groups and clusters, the Local Universe could be typical or even

  11. Extended quantification of the generalized recurrence plot

    Science.gov (United States)

    Riedl, Maik; Marwan, Norbert; Kurths, Jürgen

    2016-04-01

    The generalized recurrence plot is a modern tool for quantification of complex spatial patterns. Its application spans the analysis of trabecular bone structures, Turing structures, turbulent spatial plankton patterns, and fractals. But, it is also successfully applied to the description of spatio-temporal dynamics and the detection of regime shifts, such as in the complex Ginzburg-Landau- equation. The recurrence plot based determinism is a central measure in this framework quantifying the level of regularities in temporal and spatial structures. We extend this measure for the generalized recurrence plot considering additional operations of symmetry than the simple translation. It is tested not only on two-dimensional regular patterns and noise but also on complex spatial patterns reconstructing the parameter space of the complex Ginzburg-Landau-equation. The extended version of the determinism resulted in values which are consistent to the original recurrence plot approach. Furthermore, the proposed method allows a split of the determinism into parts which based on laminar and non-laminar regions of the two-dimensional pattern of the complex Ginzburg-Landau-equation. A comparison of these parts with a standard method of image classification, the co-occurrence matrix approach, shows differences especially in the description of patterns associated with turbulence. In that case, it seems that the extended version of the determinism allows a distinction of phase turbulence and defect turbulence by means of their spatial patterns. This ability of the proposed method promise new insights in other systems with turbulent dynamics coming from climatology, biology, ecology, and social sciences, for example.

  12. Implementing extended observing at the JCMT

    Science.gov (United States)

    Walther, Craig; Dempsey, Jessica; Campbell, Ian

    2014-08-01

    The James Clerk Maxwell Telescope (JCMT) is the largest single dish submillimetre telescope in the world. Recently the Joint Astronomy Centre (JAC) has learned that the JCMT will no longer receive financial support from its original supporting agencies after September 2014. There is significant pressure to complete some surveys that have been in progress at the JCMT for many years now. With the goal of completing a higher percentage of these surveys it was decided to take advantage of the hours between when the telescope operator leaves the telescope and when the day crew arrives. These hours generally have reasonable seeing and low column integrated water vapor, so they are good for observing. This observing is being performed remotely, in Hilo, without staff at the telescope, by staff members who do not have telescope operation as part of their job descriptions. This paper describes the hardware changes necessary to implement remote observing at JCMT. It also describes the software needed for remote, fail safe, operation of the telescope. The protocols and rules for passing the control of the telescope between the various groups are discussed. Since these Extended Operators are not expert telescope operators, the system was simplified as much as possible, but some training was necessary and proper checklists are essential. Due to the success of the first phase of Extending Observing at the JCMT, the hours when the weather is good and no one is at the telescope, but no day crew is on the way, are also now being utilized. Extended Observing has already yielded a considerable amount of science observing time.

  13. Extended technicolor contribution to the Zbb vertex

    CERN Document Server

    Hagiwara, K; Hagiwara, Kaoru; Kitazawa, Noriaki

    1995-01-01

    We show that the flavor-diagonal gauge boson of the extended technicolor theory contributes with opposite sign to the standard model correction for the Zbb vertex. This mechanism can naturally explain the deviation of the LEP result from the standard model prediction for the partial width \\Gamma(Z \\rightarrow b{\\bar b}). A smaller value of the QCD coupling, \\alpha_s(m_Z) \\simeq 0.115, is then preferred by the \\Gamma(Z \\rightarrow \\mbox{hadron}) data, which is consistent with both the recent Lattice-QCD estimate and the Particle Data Group average.

  14. Congruence Permutable Symmetric Extended de Morgan Algebras

    Institute of Scientific and Technical Information of China (English)

    Jie FANG

    2006-01-01

    An algebra A is said to be congruence permutable if any two congruences on it are per-mutable. This property has been investigated in several varieties of algebras, for example, de Morgan algebras, p-algebras, Kn,o-algebras. In this paper, we study the class of symmetric extended de Morgan algebras that are congruence permutable. In particular we consider the case where A is finite, and show that A is congruence permutable if and only if it is isomorphic to a direct product of finitely many simple algebras.

  15. Extendable retractable telescopic mast for deployable structures

    Science.gov (United States)

    Schmid, M.; Aguirre, M.

    1986-01-01

    The Extendable and Retractable Mast (ERM) which is presently developed by Dornier in the frame of an ESA-contract, will be used to deploy and retract large foldable structures. The design is based on a telescopic carbon-fiber structure with high stiffness, strength and pointing accuracy. To verify the chosen design, a breadboard model of an ERM was built and tested under thermal vacuum (TV)-conditions. It is planned as a follow-on development to manufacture and test an Engineering Model Mast. The Engineering Model will be used to establish the basis for an ERM-family covering a wide range of requirements.

  16. Extended Analysis on New Generalized Chaplygin Gas

    Institute of Scientific and Technical Information of China (English)

    WANG Jun; WU Ya-Bo; WANG Di; YANG Wei-Qiang

    2009-01-01

    We extend the study of the new generalizeal Chaplygin gas (NGCG) based on [J.Cosmol.Astropart.Phys.0601 (2006) 003].Specifically,we not only discuss the change rates of the energy densities and the energy transfer of this model,but also perform the Om diagnostic to differentiate the ACDM model from the NGCG and the GCG models.Furthermore,in order to consider the influence of dark energy on structure formation,we also present the evolution of the growth index in this scenario with interaction.

  17. Extended HI disks in nearby spiral galaxies

    Science.gov (United States)

    Bosma, Albert

    2017-03-01

    In this short write-up, I will concentrate on a few topics of interest. In the 1970s I found very extended HI disks in galaxies such as NGC 5055 and NGC 2841, out to 2 - 2.5 times the Holmberg radius. Since these galaxies are warped, a ``tilted ring model'' allows rotation curves to be derived, and evidence for dark matter to be found. The evaluation of the amount of dark matter is hampered by a disk-halo degeneracy, which can possibly be broken by observations of velocity dispersions in both the MgI region and the CaII region.

  18. Extended HI disks in nearby spiral galaxies

    CERN Document Server

    Bosma, A

    2016-01-01

    In this short write-up, I will concentrate on a few topics of interest. In the 1970s I found very extended HI disks in galaxies such as NGC 5055 and NGC 2841, out to 2 - 2.5 times the Holmberg radius. Since these galaxies are warped, a "tilted ring model" allows rotation curves to be derived, and evidence for dark matter to be found. The evaluation of the amount of dark matter is hampered by a disk-halo degeneracy, which can possibly be broken by observations of velocity dispersions in both the MgI region and the CaII region.

  19. Self-gravitating systems in Extended Gravity

    CERN Document Server

    Stabile, A

    2014-01-01

    Starting from the weak field limit, we discuss astrophysical applications of Extended Theories of Gravity where higher order curvature invariants and scalar fields are considered by generalizing the Hilbert-Einstein action linear in the Ricci curvature scalar $R$. Results are compared to General Relativity in the hypothesis that Dark Matter contributions to the dynamics can be neglected thanks to modified gravity. In particular, we consider stellar hydrostatic equilibrium, galactic rotation curves, and gravitational lensing. Finally, we discuss the weak field limit in the Jordan and Einstein frames pointing out how effective quantities, as gravitational potentials, transform from one frame to the other and the interpretation of results can completely change accordingly.

  20. Extended tree-level gauge mediation

    DEFF Research Database (Denmark)

    Monaco, M.; Nardecchia, M.; Romanino, A.;

    2011-01-01

    Tree-level gauge mediation (TGM) is a scenario of SUSY breaking in which the tree-level exchange of heavy (possibly GUT) vector fields generates flavor-universal sfermion masses. In this work we extend this framework to the case of E(6) that is the natural extension of the minimal case studied so...... if the gauge group does not contain SU(5). If SUSY breaking is mediated purely by the U(1) generator that commutes with SO(10) we obtain universal sfermion masses and thus can derive the CMSSM boundary conditions in a novel scenario....

  1. Cambridge IGCSE mathematics core and extended

    CERN Document Server

    Pimentel, Ric

    2013-01-01

    The most cost effective and straightforward way to teach the revised syllabus, with all the core and extended content covered by a single book and accompanying free digital resources.  . This title has been written for the revised Cambridge IGCSE Mathematics (0580) syllabus, for first teaching from 2013.  . ·         Gives students the practice they require to deepen their understanding through plenty of questions. ·         Consolidates learning with unique digital resources on the CD, included free with every Student's Book.  . We are working with Cambridge International Examinations to gain

  2. EXTENDED CASIMIR APPROACH TO CONTROLLED HAMILTONIAN SYSTEMS

    Institute of Scientific and Technical Information of China (English)

    Yuqian GUO; Daizhan CHENG

    2006-01-01

    In this paper, we first propose an extended Casimir method for energy-shaping. Then it is used to solve some control problems of Hamiltonian systems. To solve the H∞ control problem, the energy function of a Hamiltonian system is shaped to such a form that could be a candidate solution of HJI inequality. Next, the energy function is shaped as a candidate of control ISS-Lyapunov function, and then the input-to-state stabilization of port-controlled Hamiltonian systems is achieved. Some easily verifiable sufficient conditions are presented.

  3. Noether symmetries in extended gravity quantum cosmology

    CERN Document Server

    Capozziello, Salvatore

    2013-01-01

    We summarize the use of Noether symmetries in Minisuperspace Quantum Cosmology. In particular, we consider minisuperspace models, showing that the existence of conserved quantities gives selection rules that allow to recover classical behaviors in cosmic evolution according to the so called Hartle criterion. Such a criterion selects correlated regions in the configuration space of dynamical variables whose meaning is related to the emergence of classical observable universes. Some minisuperspace models are worked out starting from Extended Gravity, in particular coming from scalar tensor, f(R) and f(T) theories. Exact cosmological solutions are derived.

  4. Improving epidemic control strategies by extended detection

    Science.gov (United States)

    Karp, Paweł; Dybiec, Bartłomiej; Kleczkowski, Adam

    2014-11-01

    Majority of epidemics eradication programs work in preventive responsive way. The lack of exact information about epidemiological status of individuals makes responsive actions less efficient. Here, we demonstrate that additional tests can significantly increase the efficiency of "blind" treatment (vaccination or culling). Eradication strategy consisting of "blind" treatment in very limited local neighborhood supplemented by extra tests in a little bit larger neighborhood is able to prevent invasion of even highly infectious diseases and to achieve this at a cost lower than for the "blind" strategy. The effectiveness of the extended strategy depends on such parameters as the test efficiency and test cost.

  5. Extending maps between pre-uniform spaces

    OpenAIRE

    Adalberto García-Máynez; Rubén Mancio-Toledo

    2012-01-01

    [EN] We give sufficient conditions on a uniformly continuous map f: (X,U) → (Y, V ) between completable T1-pre-uniform spaces (X,U), (Y, V ) to have a continuous or a uniformly continuous extension f:X → Y between the corresponding completions. García-Máynez, A.; Mancio-Toledo, R. (2012). Extending maps between pre-uniform spaces. Applied General Topology. 13(1):21-25. doi:10.4995/agt.2012.1634. 21 25 13 1

  6. Extended Global Convergence Framework for Unconstrained Optimization

    Institute of Scientific and Technical Information of China (English)

    (A)rpád B(U)RMEN; Franc BRATKOVI(C); Janez PUHAN; Iztok FAJFAR; Tadej TUMA

    2004-01-01

    An extension of the global convergence framework for unconstrained derivative-free optimization methods is presented. The extension makes it possible for the framework to include optimization methods with varying cardinality of the ordered direction set. Grid-based search methods are shown to be a special case of the more general extended global convergence framework. Furthermore,the required properties of the sequence of ordered direction sets listed in the definition of grid-based methods are re]axed and simplified by removing the requirement of structural equivalence.

  7. Assurance and US extended deterrence in NATO

    OpenAIRE

    Yost, David S.

    2009-01-01

    The NATO allies agreed at the Strasbourg/Kehl summit in April 2009 to prepare a new Strategic Concept for approval at their next summit. One of the issues in the Strategic Concept review will be the alliance’s nuclear deterrence posture and policy. While three members of the alliance (Britain, France and the United States) are nuclear powers, historically the greatest amount of attention has been focused on US ‘extended deterrence’—that is, the extension by Washington of an ...

  8. Resonance regions of extended Mathieu equation

    Science.gov (United States)

    Semyonov, V. P.; Timofeev, A. V.

    2016-02-01

    One of the mechanisms of energy transfer between degrees of freedom of dusty plasma system is based on parametric resonance. Initial stage of this process can de described by equation similar to Mathieu equation. Such equation is studied by analytical and numerical approach. The numerical solution of the extended Mathieu equation is obtained for a wide range of parameter values. Boundaries of resonance regions, growth rates of amplitudes and times of onset are obtained. The energy transfer between the degrees of freedom of dusty plasma system can occur over a wide range of frequencies.

  9. Simulating futures in extended common LISP

    Science.gov (United States)

    Nachtsheim, Philip R.

    1988-01-01

    Stack-groups comprise the mechanism underlying implementation of multiprocessing in Extended Common LISP, i.e., running multiple quasi-simultaneous processes within a single LISP address space. On the other hand, the future construct of MULTILISP, an extension of the LISP dialect scheme, deals with parallel execution. The source of concurrency that future exploits is the overlap between computation of a value and use of the value. Described is a simulation of the future construct by an interpreter utilizing stack-group extensions to common LISP.

  10. Primordial fluctuations in extended Liouville theory

    Science.gov (United States)

    Moore, Wynton E.

    2015-03-01

    Liouville gravity can be used to precisely model features of 3+1 dimensional cosmology in a simplified 1+1d setting. We study primordial fluctuations in a generally covariant extension of Liouville theory, in the context of single field inflation. The scale invariant spectrum of scalar curvature perturbations is exhibited, and their three-point correlation function is computed in the slow roll approximation. We recover Maldacena's consistency relation for the three-point function, which in this context depends on a global shift symmetry of extended Liouville theory.

  11. Primordial fluctuations in extended Liouville theory

    CERN Document Server

    Moore, Wynton E

    2014-01-01

    Liouville gravity can be used to precisely model features of 3+1 dimensional cosmology in a simplified 1+1d setting. We study primordial fluctuations in a generally covariant extension of Liouville theory, in the context of single field inflation. The scale invariant spectrum of scalar curvature perturbations is exhibited, and their three-point correlation function is computed in the slow roll approximation. We recover Maldacena's consistency relation for the three-point function, which in this context depends on a global shift symmetry of extended Liouville theory.

  12. Optimal guidance of extended trajectory shaping

    Institute of Scientific and Technical Information of China (English)

    Wang Hui; Lin Defu; Cheng Zhenxuan; Wang Jiang

    2014-01-01

    To control missile’s miss distance as well as terminal impact angle, by involving the time-to-go-nth power in the cost function, an extended optimal guidance law against a constant maneu-vering target or a stationary target is proposed using the linear quadratic optimal control theory. An extended trajectory shaping guidance (ETSG) law is then proposed under the assumption that the missile-target relative velocity is constant and the line of sight angle is small. For a lag-free ETSG system, closed-form solutions for the missile’s acceleration command are derived by the method of Schwartz inequality and linear simulations are performed to verify the closed-form results. Normalized adjoint systems for miss distance and terminal impact angle error are presented independently for stationary targets and constant maneuvering targets, respectively. Detailed discussions about the terminal misses and impact angle errors induced by terminal impact angle constraint, initial heading error, seeker zero position errors and target maneuvering, are performed.

  13. Fundamentals of Physics, Extended 7th Edition

    Science.gov (United States)

    Halliday, David; Resnick, Robert; Walker, Jearl

    2004-05-01

    No other book on the market today can match the 30-year success of Halliday, Resnick and Walker's Fundamentals of Physics! Fundamentals of Physics, 7th Edition and the Extended Version, 7th Edition offer a solid understanding of fundamental physics concepts, helping readers apply this conceptual understanding to quantitative problem solving, in a breezy, easy-to-understand style. A unique combination of authoritative content and stimulating applications. * Numerous improvements in the text, based on feedback from the many users of the sixth edition (both instructors and students) * Several thousand end-of-chapter problems have been rewritten to streamline both the presentations and answers * 'Chapter Puzzlers' open each chapter with an intriguing application or question that is explained or answered in the chapter * Problem-solving tactics are provided to help beginning Physics students solve problems and avoid common error * The first section in every chapter introduces the subject of the chapter by asking and answering, "What is Physics?" as the question pertains to the chapter * Numerous supplements available to aid teachers and students The extended edition provides coverage of developments in Physics in the last 100 years, including: Einstein and Relativity, Bohr and others and Quantum Theory, and the more recent theoretical developments like String Theory.

  14. Extending Teach and Repeat to Pivoting Wheelchairs

    Directory of Open Access Journals (Sweden)

    Guillermo Del Castillo

    2003-02-01

    Full Text Available The paper extends the teach-and-repeat paradigm that has been successful for the control of holonomic robots to nonholonomic wheelchairs which may undergo pivoting action over the course of their taught movement. Due to the nonholonomic nature of the vehicle kinematics, estimation is required -- in the example given herein, based upon video detection of wall-mounted cues -- both in the teaching and the tracking events. In order to accommodate motion that approaches pivoting action as well as motion that approaches straight-line action, the estimation equations of the Extended Kalman Filter and the control equations are formulated using two different definitions of a nontemporal independent variable. The paper motivates the need for pivoting action in real-life settings by reporting extensively on the abilities and limitations of estimation-based teach-and-repeat action where pivoting and near-pivoting action is disallowed. Following formulation of the equations in the near-pivot mode, the paper reports upon experiments where taught trajectories which entail a seamless mix of near-straight and near-pivot action are tracked.

  15. Extended arrays for nonlinear susceptibility magnitude imaging

    Science.gov (United States)

    Ficko, Bradley W.; Giacometti, Paolo; Diamond, Solomon G.

    2016-01-01

    This study implements nonlinear susceptibility magnitude imaging (SMI) with multifrequency intermodulation and phase encoding. An imaging grid was constructed of cylindrical wells of 3.5-mm diameter and 4.2-mm height on a hexagonal two-dimensional 61-voxel pattern with 5-mm spacing. Patterns of sample wells were filled with 40-μl volumes of Fe3O4 starch-coated magnetic nanoparticles (mNPs) with a hydrodynamic diameter of 100 nm and a concentration of 25 mg/ml. The imaging hardware was configured with three excitation coils and three detection coils in anticipation that a larger imaging system will have arrays of excitation and detection coils. Hexagonal and bar patterns of mNP were successfully imaged (R2 > 0.9) at several orientations. This SMI demonstration extends our prior work to feature a larger coil array, enlarged field-of-view, effective phase encoding scheme, reduced mNP sample size, and more complex imaging patterns to test the feasibility of extending the method beyond the pilot scale. The results presented in this study show that nonlinear SMI holds promise for further development into a practical imaging system for medical applications. PMID:26124044

  16. Quasicontinuous functions, domains, and extended calculus

    Directory of Open Access Journals (Sweden)

    Rodica Cazacu

    2007-04-01

    Full Text Available One of the aims of domain theory is the construction of an embedding of a given structure or data type as the maximal or “ideal” elements of an enveloping domain of “approximations,” sometimes called a domain environment. Typically the goal is to provide a computational model or framework for recursive and algorithmic reasoning about the original structure. In this paper we consider the function space of (natural equivalence classes of quasicontinuous functions from a locally compact space X into L, an n-fold product of the extended reals [−1,1] (more generally, into a bicontinuous lattice. We show that the domain of all “approximate maps” that assign to each point of X an order interval of L is a domain environment for the quasicontinuous function space. We rely upon the theory of domain environments to introduce an interesting and useful function space topology on the quasicontinuous function space. We then apply this machinery to define an extended differential calculus in the quasicontinuous function space, and draw connections with viscosity solutions of Hamiltonian equations. The theory depends heavily on topological properties of quasicontinuous functions that have been recently uncovered that involve dense sets of points of continuity and sections of closed relations and USCO maps. These and other basic results about quasicontinuous functions are surveyed and presented in the early sections.

  17. Extended parental care in communal social groups

    Directory of Open Access Journals (Sweden)

    Stephen H. Forbes

    2002-11-01

    Full Text Available Recent developments in social insect research have challenged the need for close kinship as a prerequisite for the evolution of stable group living. In a model communal bee species, Lasioglossum (Chilalictus hemichalceum, previous allozyme work indicated that groups of cooperating adult females are not relatives. Yet at any given time, not all group members perform the risky task of foraging. We previously hypothesized that tolerance for non-foragers was a component of extended parental care, previously known only for kin based social systems. DNA microsatellites were used to study colony genetic structure in order to test this hypothesis. Microsatellite polymorphism was substantial (He = 0.775. Overall intracolony relatedness, mainly of immatures, was low but significant in nine, late season nests (r = 0.136 plus or minus0.023, indicating that broods contain five to six unrelated sib ships. Detailed analyses of kinship between pairs of individuals revealed that most pairs were unrelated and most related pairs were siblings. Mothers are absent for 89-91% of the developing immature females, and 97% of developing males. Alternatively, 46% of adult females had neither sibs nor offspring in their nests. These findings indicate that the extended parental care model applies broadly to both kin based and nonkin based social systems in the Hymenoptera.

  18. Thermoplastic Polyurethanes with Isosorbide Chain Extender

    Energy Technology Data Exchange (ETDEWEB)

    Javni, Ivan; Bilic, Olivera; Bilic, Nikola; Petrovic, Zoran; Eastwood, Eric; Zhang, Fan; Ilavsky, Jan

    2015-12-15

    Isosorbide, a renewable diol derived from starch, was used alone or in combination with butane diol (BD) as the chain extender in two series of thermoplastic polyurethanes (TPU) with 50 and 70% polytetramethylene ether glycol (PTMEG) soft segment concentration (SSC), respectively. In the synthesized TPUs, the hard segment composition was systematically varied in both series following BD/isosorbide molar ratios of 100 : 0; 75 : 25; 50 : 50; 25 : 75, and 0 : 100 to examine in detail the effect of chain extenders on properties of segmented polyurethane elastomers with different morphologies. We found that polyurethanes with 50% SSC were hard elastomers with Shore D hardness of around 50, which is consistent with assumed co-continuous morphology. Polymers with 70% SSC displayed lower Shore A hardness of 74–79 (Shore D around 25) as a result of globular hard domains dispersed in the soft matrix. Insertion of isosorbide increased rigidity, melting point and glass transition temperature of hard segments and tensile strength of elastomers with 50% SSC. These effects were weaker or non-existent in 70% SSC series due to the short hard segments and low content of isosorbide. We also found that the thermal stability was lowered by increasing isosorbide content in both series.

  19. Extending Conceptual Schemas with Business Process Information

    Directory of Open Access Journals (Sweden)

    Marco Brambilla

    2010-01-01

    Full Text Available The specification of business processes is becoming a more and more critical aspect for organizations. Such processes are specified as workflow models expressing the logical precedence among the different business activities (i.e., the units of work. Typically, workflow models are managed through specific subsystems, called workflow management systems, to ensure a consistent behavior of the applications with respect to the organization business process. However, for small organizations and/or simple business processes, the complexity and capabilities of these dedicated workflow engines may be overwhelming. In this paper, we therefore, advocate for a different and lightweight approach, consisting in the integration of the business process specification within the system conceptual schema. We show how a workflow-extended conceptual schema can be automatically obtained, which serves both to enforce the organization business process and to manage all its relevant domain data in a unified way. This extended model can be directly processed with current CASE tools, for instance, to generate an implementation of the system (including its business process in any technological platform.

  20. Differential Poisson Sigma Models with Extended Supersymmetry

    CERN Document Server

    Arias, Cesar; Torres-Gomez, Alexander

    2016-01-01

    The induced two-dimensional topological N=1 supersymmetric sigma model on a differential Poisson manifold M presented in arXiv:1503.05625 is shown to be a special case of the induced Poisson sigma model on the bi-graded supermanifold T[0,1]M. The bi-degree comprises the standard N-valued target space degree, corresponding to the form degree on the worldsheet, and an additional Z-valued fermion number, corresponding to the degree in the differential graded algebra of forms on M. The N=1 supersymmetry stems from the compatibility between the (extended) differential Poisson bracket and the de Rham differential on M. The latter is mapped to a nilpotent vector field Q of bi-degree (0,1) on T*[1,0](T[0,1]M), and the covariant Hamiltonian action is Q-exact. New extended supersymmetries arise as inner derivatives along special bosonic Killing vectors on M that induce Killing supervector fields of bi-degree (0,-1) on T*[1,0](T[0,1]M).

  1. Agents in grid extended to clouds

    Science.gov (United States)

    Wasielewska, K.; Ganzha, M.; Paprzycki, M.; Bǎdicǎ, C.; Ivanovic, M.; Lirkov, I.; Fidanova, S.

    2016-10-01

    The presented work is an attempt to extend considerations from the Agents in Grid (AiG) project to the Clouds. The AiG project is aimed at the development of an agent-semantic infrastructure for efficient resource management in the grid. Decision support within the AIG system helps the user, without in-depth knowledge, to choose optimal algorithm and/or resource to solve a problem from a given domain, and later to choose the best contract defining terms of collaboration with the provider of a resource used to solve the problem. Cloud computing refers to an architecture, in which groups of remote servers are networked, to allow online access to computer services or resources. The general vision is the same as in the case of computational grids, i.e., to reduce cost of computing, as well as to increase flexibility and reliability of the infrastructure. However, there are also important differences. It is relatively easy to notice that solutions considered in the context of the AiG system can be easily extended to computational clouds that evolved from computational grids. As it was shown in the case of grids, integrating software agents, semantics and cloud computing could enable highly efficient, intelligent systems, making clouds even more flexible, autonomic and usable.

  2. Charged Local Defects in Extended Systems

    Energy Technology Data Exchange (ETDEWEB)

    Schultz, Peter A.

    1999-05-25

    The conventional approach to treating charged defects in extended systems in first principles calculations is via the supercell approximation using a neutralizing jellium background charge. I explicitly demonstrate shortcomings of this standard approach and discuss the consequences. Errors in the electrostatic potential surface over the volume of a supercell are shown to be comparable to a band gap energy in semiconductor materials, for cell sizes typically used in first principles simulations. I present an alternate method for eliminating the divergence of the Coulomb potential in supercell calculations of charged defects in extended systems that embodies a correct treatment of the electrostatic potential in the local viciniq of the a charged defect, via a mixed boundary condition approach. I present results of first principles calculations of charged vacancies in NaCl that illustrate the importance of polarization effects once an accurate representation of the local potential is obtained. These polarization effects, poorly captured in small supercells, also impact the energetic on the scale of typical band gap energies.

  3. Mechanics of extended masses in general relativity

    CERN Document Server

    Harte, Abraham I

    2011-01-01

    The "external" or "bulk" motion of extended bodies is studied in general relativity. Material objects of arbitrary shape, spin, internal composition, and velocity are allowed as long as the metric remains near a vacuum solution (with a possible cosmological constant). Under this restriction, physically reasonable linear and angular momenta are proposed that evolve as though they were the momenta of an extended test body moving in an effective vacuum metric. This result holds to all multipole orders. The portion of the physical metric that does not directly affect the motion is a slightly generalized form of the Detweiler-Whiting S-field originally introduced in the context of self-force. This serves only to (finitely) renormalize the "bare" multipole moments of the object's stress-energy tensor. The MiSaTaQu expression for the gravitational self-force is recovered as a simple application. A gravitational self-torque is obtained as well. Lastly, a certain exact result is derived that may provide a basis for un...

  4. A Novel Biped Pattern Generator Based on Extended ZMP and Extended Cart-table Model

    Directory of Open Access Journals (Sweden)

    Guangbin Sun

    2015-07-01

    Full Text Available This paper focuses on planning patterns for biped walking on complex terrains. Two problems are solved: ZMP (zero moment point cannot be used on uneven terrain, and the conventional cart-table model does not allow vertical CM (centre of mass motion. For the ZMP definition problem, we propose the extended ZMP (EZMP concept as an extension of ZMP to uneven terrains. It can be used to judge dynamic balance on universal terrains. We achieve a deeper insight into the connection and difference between ZMP and EZMP by adding different constraints. For the model problem, we extend the cart-table model by using a dynamic constraint instead of constant height constraint, which results in a mathematically symmetric set of three equations. In this way, the vertical motion is enabled and the resultant equations are still linear. Based on the extended ZMP concept and extended cart-table model, a biped pattern generator using triple preview controllers is constructed and implemented simultaneously to three dimensions. Using the proposed pattern generator, the Atlas robot is simulated. The simulation results show the robot can walk stably on rather complex terrains by accurately tracking extended ZMP.

  5. Quantum gravity kinematics from extended TQFTs

    Science.gov (United States)

    Dittrich, Bianca; Geiller, Marc

    2017-01-01

    In this paper, we show how extended topological quantum field theories (TQFTs) can be used to obtain a kinematical setup for quantum gravity, i.e. a kinematical Hilbert space together with a representation of the observable algebra including operators of quantum geometry. In particular, we consider the holonomy-flux algebra of (2 + 1)-dimensional Euclidean loop quantum gravity, and construct a new representation of this algebra that incorporates a positive cosmological constant. The vacuum state underlying our representation is defined by the Turaev-Viro TQFT. This vacuum state can be thought of as being peaked on connections with homogeneous curvature. We therefore construct here a generalization, or more precisely a quantum deformation at root of unity, of the previously introduced SU(2) BF representation. The extended Turaev-Viro TQFT provides a description of the excitations on top of the vacuum. These curvature and torsion excitations are classified by the Drinfeld center category of the quantum deformation of SU(2), and are essential in order to allow for a representation of the holonomies and fluxes. The holonomies and fluxes are generalized to ribbon operators which create and interact with the excitations. These excitations agree with the ones induced by massive and spinning particles, and therefore the framework presented here allows automatically for a description of the coupling of such matter to (2+1)-dimensional gravity with a cosmological constant. The new representation constructed here presents a number of advantages over the representations which exist so far. In particular, it possesses a very useful finiteness property which guarantees the discreteness of spectra for a wide class of quantum (intrinsic and extrinsic) geometrical operators. Also, the notion of basic excitations leads to a so-called fusion basis which offers exciting possibilities for the construction of states with interesting global properties, as well as states with certain

  6. Extended Forward Sensitivity Analysis for Uncertainty Quantification

    Energy Technology Data Exchange (ETDEWEB)

    Haihua Zhao; Vincent A. Mousseau

    2008-09-01

    This report presents the forward sensitivity analysis method as a means for quantification of uncertainty in system analysis. The traditional approach to uncertainty quantification is based on a “black box” approach. The simulation tool is treated as an unknown signal generator, a distribution of inputs according to assumed probability density functions is sent in and the distribution of the outputs is measured and correlated back to the original input distribution. This approach requires large number of simulation runs and therefore has high computational cost. Contrary to the “black box” method, a more efficient sensitivity approach can take advantage of intimate knowledge of the simulation code. In this approach equations for the propagation of uncertainty are constructed and the sensitivity is solved for as variables in the same simulation. This “glass box” method can generate similar sensitivity information as the above “black box” approach with couples of runs to cover a large uncertainty region. Because only small numbers of runs are required, those runs can be done with a high accuracy in space and time ensuring that the uncertainty of the physical model is being measured and not simply the numerical error caused by the coarse discretization. In the forward sensitivity method, the model is differentiated with respect to each parameter to yield an additional system of the same size as the original one, the result of which is the solution sensitivity. The sensitivity of any output variable can then be directly obtained from these sensitivities by applying the chain rule of differentiation. We extend the forward sensitivity method to include time and spatial steps as special parameters so that the numerical errors can be quantified against other physical parameters. This extension makes the forward sensitivity method a much more powerful tool to help uncertainty analysis. By knowing the relative sensitivity of time and space steps with other

  7. Extended MRI findings of intersection syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Roger P.; Hatem, Stephen F.; Recht, Michael P. [Cleveland Clinic, Cleveland, OH (United States)

    2009-02-15

    The symptoms and physical findings of intersection syndrome have been well described in the clinical medical literature. However, the magnetic resonance imaging (MRI) findings in patients with intersection syndrome of the forearm have only recently been described in a small number of patients. We review our experience with imaging of intersection syndrome, describe previously unreported MRI findings, and emphasize modifications to MRI protocols for its evaluation. Institutional review board approval was obtained for this retrospective review of patients with MRI findings consistent with intersection syndrome of the forearm during the period from January 2004 to September 2006. Six patients were identified, three males and three females, with an average age of 39.3 years. The MRI examinations were reviewed to assess signal abnormalities within and adjacent to the first and second dorsal extensor tendon compartments (DETC): tendinosis, peritendinous edema or fluid, muscle edema, subcutaneous edema, and juxtacortical edema. The overall longitudinal extent of signal alterations was measured as well as the distance from Lister's tubercle to the crossover of the first and second DETC. Review of the MRIs showed increased intrasubstance tendon signal suggesting tendinosis in two of the six patients, peritendinous edema or fluid in all six patients, muscle edema in five of the six patients, and subcutaneous edema in three of the six patients. Juxtacortical edema was seen in one patient. Peritendinous edema or fluid extended distally beyond the radiocarpal joint in three of the six patients. The average distance from Lister's tubercle to the crossover of the first and second DETC was 3.95 cm, in keeping with recently published data. Intersection syndrome is an uncommon MRI diagnosis. In addition to the previously described MRI findings of edema adjacent to the first or second DETC, possibly with proximal extension and subcutaneous edema, we have identified

  8. Ongoing Mars Missions: Extended Mission Plans

    Science.gov (United States)

    Zurek, Richard; Diniega, Serina; Crisp, Joy; Fraeman, Abigail; Golombek, Matt; Jakosky, Bruce; Plaut, Jeff; Senske, David A.; Tamppari, Leslie; Thompson, Thomas W.; Vasavada, Ashwin R.

    2016-10-01

    Many key scientific discoveries in planetary science have been made during extended missions. This is certainly true for the Mars missions both in orbit and on the planet's surface. Every two years, ongoing NASA planetary missions propose investigations for the next two years. This year, as part of the 2016 Planetary Sciences Division (PSD) Mission Senior Review, the Mars Odyssey (ODY) orbiter project submitted a proposal for its 7th extended mission, the Mars Exploration Rover (MER-B) Opportunity submitted for its 10th, the Mars Reconnaissance Orbiter (MRO) for its 4th, and the Mars Science Laboratory (MSL) Curiosity rover and the Mars Atmosphere and Volatile Evolution (MVN) orbiter for their 2nd extended missions, respectively. Continued US participation in the ongoing Mars Express Mission (MEX) was also proposed. These missions arrived at Mars in 2001, 2004, 2006, 2012, 2014, and 2003, respectively. Highlights of proposed activities include systematic observations of the surface and atmosphere in twilight (early morning and late evening), building on a 13-year record of global mapping (ODY); exploration of a crater rim gully and interior of Endeavour Crater, while continuing to test what can and cannot be seen from orbit (MER-B); refocused observations of ancient aqueous deposits and polar cap interiors, while adding a 6th Mars year of change detection in the atmosphere and the surface (MRO); exploration and sampling by a rover of mineralogically diverse strata of Mt. Sharp and of atmospheric methane in Gale Crater (MSL); and further characterization of atmospheric escape under different solar conditions (MVN). As proposed, these activities follow up on previous discoveries (e.g., recurring slope lineae, habitable environments), while expanding spatial and temporal coverage to guide new detailed observations. An independent review panel evaluated these proposals, met with project representatives in May, and made recommendations to NASA in June 2016. In this

  9. Extending Stochastic Network Calculus to Loss Analysis

    Directory of Open Access Journals (Sweden)

    Chao Luo

    2013-01-01

    Full Text Available Loss is an important parameter of Quality of Service (QoS. Though stochastic network calculus is a very useful tool for performance evaluation of computer networks, existing studies on stochastic service guarantees mainly focused on the delay and backlog. Some efforts have been made to analyse loss by deterministic network calculus, but there are few results to extend stochastic network calculus for loss analysis. In this paper, we introduce a new parameter named loss factor into stochastic network calculus and then derive the loss bound through the existing arrival curve and service curve via this parameter. We then prove that our result is suitable for the networks with multiple input flows. Simulations show the impact of buffer size, arrival traffic, and service on the loss factor.

  10. Extended Magnetic Reconnection Across the Dayside Magnetopause

    Science.gov (United States)

    Dunlop, M. W.; Zhang, Q.-H.; Bogdanova, Y. V.; Lockwood, M.; Pu, Z.; Hasegawa, H.; Wang, J.; Taylor, M. G. G. T.; Berchem, J.; Lavraund, B.; Eastwood, J.; Volwerk, M.; Shen, C.; Shi, J.-K.; Constantinescu, D.; Frey, H.; Fazakerley, A. N.; Sibeck, D.; Escoubet, P.; Wild, J. A.; Liu, Z.-X.

    2011-01-01

    The extent of where magnetic reconnection (MR), the dominant process responsible for energy and plasma transport into the magnetosphere, operates across Earth's dayside magnetopause has previously been only indirectly shown by observations. We report the first direct evidence of X-line structure resulting from the operation of MR at each of two widely separated locations along the tilted, subsolar line of maximum current on Earth's magnetopause, confirming the operation of MR at two or more sites across the extended region where MR is expected to occur. The evidence results from in-situ observations of the associated ion and electron plasma distributions, present within each magnetic X-line structure, taken by two spacecraft passing through the active MR regions simultaneously.

  11. Extended Lagrange interpolation in L1 spaces

    Science.gov (United States)

    Occorsio, Donatella; Russo, Maria Grazia

    2016-10-01

    Let w (x )=e-xβxα , w ¯(x )=x w (x ) and denote by {pm(w)}m,{pn(w¯)}n the corresponding sequences of orthonormal polynomials. The zeros of the polynomial Q2 m +1=pm +1(w )pm(w ¯) are simple and are sufficiently far among them. Therefore it is possible to construct an interpolation process essentially based on the zeros of Q2m+1, which is called "Extended Lagrange Interpolation". Here we study the convergence of this interpolation process in suitable weighted L1 spaces. This study completes the results given by the authors in previous papers in weighted Lup((0 ,+∞ )) , for 1≤p≤∞. Moreover an application of the proposed interpolation process in order to construct an e cient product quadrature scheme for weakly singular integrals is given.

  12. FUZZY OPTIMIZATION USING EXTENDED KALMAN FILTER

    Directory of Open Access Journals (Sweden)

    M.DIVYA

    2013-01-01

    Full Text Available Fuzzy Logic is based on the idea that in fuzzy sets each element in the set can assume a value from 0 to 1, not only 0 or 1, as in crisp set theory. The degree of membership function is defined as the gradation in the extent to which an element is belonging to the relevant sets. Optimizing the membership functions of a fuzzy system can be viewed as a system identification problem for nonlinear dynamic system. In this paper two input and one output fuzzy controller is designed for the dynamic process of aircraft. The addition of an EKF in the feedback loop improved the system response by blocking possible effects of measurement error based on Predictor-Corrector algorithm. An Extended Kalman Filter approach to optimize the membership functions of the inputs and outputs of the fuzzy controller. The performance of the fuzzy system before and after the optimization are compared, as well as the membership functions.

  13. Revisiting perturbations in extended quasidilaton massive gravity

    Science.gov (United States)

    Heisenberg, Lavinia

    2015-04-01

    In this work we study the theory of extended quasidilaton massive gravity together with the presence of matter fields. After discussing the homogeneous and isotropic fully dynamical background equations, which governs the exact expansion history of the universe, we consider small cosmological perturbations around these general FLRW solutions. The stability of tensor, vector and scalar perturbations on top of these general background solutions give rise to slightly different constraints on the parameters of the theory than those obtained in the approximative assumption of the late-time asymptotic form of the expansion history, which does not correspond to our current epoch. This opens up the possibility of stable FLRW solutions to be compared with current data on cosmic expansion with the restricted parameter space based on theoretical ground.

  14. Affordances and the musically extended mind

    Directory of Open Access Journals (Sweden)

    Joel eKrueger

    2014-01-01

    Full Text Available I defend a model of the musically extended mind. I consider how acts of musicking grant access to novel emotional experiences otherwise inaccessible. First, I discuss the idea of musical affordances and specify both what musical affordances are and how they invite different forms of entrainment. Next, I argue that musical affordances—via soliciting different forms of entrainment—enhance the functionality of various endogenous, emotion-granting regulative processes, drawing novel experiences out of us with an expanded complexity and phenomenal character. I suggest that music therefore ought to be thought of as part of the vehicle needed to realize these emotional experiences. I appeal to different sources of empirical work to develop this idea.

  15. Extended equal areas criterion: foundations and applications

    Energy Technology Data Exchange (ETDEWEB)

    Yusheng, Xue [Nanjim Automation Research Institute, Nanjim (China)

    1994-12-31

    The extended equal area criterion (EEAC) provides analytical expressions for ultra fast transient stability assessment, flexible sensitivity analysis, and means to preventive and emergency controls. Its outstanding performances have been demonstrated by thousands upon thousands simulations on more than 50 real power systems and by on-line operation records in an EMS environment of Northeast China Power System since September 1992. However, the researchers have mainly based on heuristics and simulations. This paper lays a theoretical foundation of EEAC and brings to light the mechanism of transient stability. It proves true that the dynamic EEAC furnishes a necessary and sufficient condition for stability of multi machine systems with any detailed models, in the sense of the integration accuracy. This establishes a new platform for further advancing EEAC and better understanding of problems. An overview of EEAC applications in China is also given in this paper. (author) 30 refs.

  16. Generalized extended Navier-Stokes theory

    DEFF Research Database (Denmark)

    Hansen, J. S.; Daivis, Peter J.; Dyre, Jeppe C.

    2013-01-01

    in molecular fluids. To discuss these phenomena in detail, molecular dynamics simulations of molecular chlorine are performed for three different state points. In general, the theory captures the behavior for small wavevector and frequencies as expected. For example, in the hydrodynamic regime......The extended Navier-Stokes theory accounts for the coupling between the translational and rotational molecular degrees of freedom. In this paper, we generalize this theory to non-zero frequencies and wavevectors, which enables a new study of spatio-temporal correlation phenomena present...... and for molecular fluids with small moment of inertia like chlorine, the theory predicts that the longitudinal and transverse intrinsic angular velocity correlation functions are almost identical, which is also seen in the molecular dynamics simulations. However, the theory fails at large wavevector and frequencies...

  17. An Extended Mild-Slope Equation

    Institute of Scientific and Technical Information of China (English)

    PAN Junning; HONG Guangwen; ZUO Qihua

    2000-01-01

    On the assumption that the vortex and the vertical velocity component of the current are small, a mild-slope equation for wave propagation on non-uniform flows is deduced from the basic hydrodynamic equations, with the terms of ( h h)2 and /2h h included in the equation. The terms of bottom friction, wind energy input and wave nonlinearity are also introduced into the equation. The wind energy input functions for wind waves and swells are separately considered by adopting Wen′s (1989) empirical formula for wind waves and Snyder′s observation results for swells. Thus, an extended mild-slope equation is obtained, in which the effects of refraction, diffraction, reflection, current, bottom friction, wind energy input and wave nonlinearity are considered synthetically.

  18. The Higgs boson from an extended symmetry

    CERN Document Server

    Barbieri, Riccardo; Rychkov, Vyacheslav S; Varagnolo, Alvise

    2007-01-01

    The variety of ideas put forward in the context of a "composite" picture for the Higgs boson calls for a simple and effective description of the related phenomenology. Such a description is given here by means of a "minimal" model explicitly applied to the case of a Higgs-top sector from an SO(5) symmetry. We discuss the spectrum, the ElectroWeak Precision Tests, flavor and B-physics, LHC phenomenology, and naturalness. The extended gauge sector relative to the standard SU(2)xU(1), if there is any, has little or no impact on these considerations. We also discuss the relation of the "minimal" model with its "little Higgs" or "holographic" extensions based on the same symmetry.

  19. Casimir effect in Extended Theories of Gravity

    CERN Document Server

    Lambiase, G; Stabile, An

    2016-01-01

    We study the Casimir vacuum energy density and the Casimir pressure for a massless scalar field confined between two nearby parallel plates in a slightly curved, static spacetime background, employing the weak field approximation in the framework of Extended Theories of Gravity (ETG). Following a perturbative approach upto second order, we find the gravity correction in the ETG to Casimir vacuum energy density and pressure. The corrections to the vacuum energy density in presence of curved spacetime in the framework of General Relativity (GR) are small and today they are still undetected with the current technology. However, future sensitivity improvement in gravitational interferometer experiments will give an useful tool to detect such effect induced by gravity. For these reason we retain interesting from a theoretical point of view generalize the outcomes of GR in the context of ETG. Finally, we find the general relation to constraining the free parameters of the ETG.

  20. Autopoiesis + extended cognition + nature = can buildings think?

    Science.gov (United States)

    Dollens, Dennis

    2015-01-01

    To incorporate metabolic, bioremedial functions into the performance of buildings and to balance generative architecture's dominant focus on computational programming and digital fabrication, this text first discusses hybridizing Maturana and Varela's biological theory of autopoiesis with Andy Clark's hypothesis of extended cognition. Doing so establishes a procedural protocol to research biological domains from which design could source data/insight from biosemiotics, sensory plants, and biocomputation. I trace computation and botanic simulations back to Alan Turing's little-known 1950s Morphogenetic drawings, reaction-diffusion algorithms, and pioneering artificial intelligence (AI) in order to establish bioarchitecture's generative point of origin. I ask provocatively, Can buildings think? as a question echoing Turing's own, "Can machines think?" PMID:26478784

  1. Action principles for extended magnetohydrodynamic models

    Energy Technology Data Exchange (ETDEWEB)

    Keramidas Charidakos, I.; Lingam, M.; Morrison, P. J.; White, R. L. [Institute for Fusion Studies and Department of Physics, The University of Texas at Austin, Austin, Texas 78712 (United States); Wurm, A. [Department of Physical and Biological Sciences, Western New England University, Springfield, Massachusetts 01119 (United States)

    2014-09-15

    The general, non-dissipative, two-fluid model in plasma physics is Hamiltonian, but this property is sometimes lost or obscured in the process of deriving simplified (or reduced) two-fluid or one-fluid models from the two-fluid equations of motion. To ensure that the reduced models are Hamiltonian, we start with the general two-fluid action functional, and make all the approximations, changes of variables, and expansions directly within the action context. The resulting equations are then mapped to the Eulerian fluid variables using a novel nonlocal Lagrange-Euler map. Using this method, we recover Lüst's general two-fluid model, extended magnetohydrodynamic (MHD), Hall MHD, and electron MHD from a unified framework. The variational formulation allows us to use Noether's theorem to derive conserved quantities for each symmetry of the action.

  2. Revisiting perturbations in extended quasidilaton massive gravity

    CERN Document Server

    Heisenberg, Lavinia

    2015-01-01

    In this work we study the theory of extended quasidilaton massive gravity together with the presence of matter fields. After discussing the homogeneous and isotropic fully dynamical background equations, which governs the exact expansion history of the universe, we consider small cosmological perturbations around these general FLRW solutions. The stability of tensor, vector and scalar perturbations on top of these general background solutions give rise to slightly different constraints on the parameters of the theory than those obtained in the approximative assumption of the late-time asymptotic form of the expansion history, which does not correspond to our current epoch. This opens up the possibility of stable FLRW solutions to be compared with current data on cosmic expansion with the restricted parameter space based on theoretical ground.

  3. Do we need an extended evolutionary synthesis?

    Science.gov (United States)

    Pigliucci, Massimo

    2007-12-01

    The Modern Synthesis (MS) is the current paradigm in evolutionary biology. It was actually built by expanding on the conceptual foundations laid out by its predecessors, Darwinism and neo-Darwinism. For sometime now there has been talk of a new Extended Evolutionary Synthesis (EES), and this article begins to outline why we may need such an extension, and how it may come about. As philosopher Karl Popper has noticed, the current evolutionary theory is a theory of genes, and we still lack a theory of forms. The field began, in fact, as a theory of forms in Darwin's days, and the major goal that an EES will aim for is a unification of our theories of genes and of forms. This may be achieved through an organic grafting of novel concepts onto the foundational structure of the MS, particularly evolvability, phenotypic plasticity, epigenetic inheritance, complexity theory, and the theory of evolution in highly dimensional adaptive landscapes.

  4. A noncommutative extended de Finetti theorem

    CERN Document Server

    Köstler, Claus

    2008-01-01

    The extended de Finetti theorem characterizes exchangeable infinite random sequences as conditionally i.i.d. and shows that the apparently weaker distributional symmetry of spreadability is equivalent to exchangeability. Our main result is a noncommutative version of this theorem. In contrast to the classical result of Ryll-Nadzewski, exchangeability turns out to be stronger than spreadability for infinite noncommutative random sequences. Out of our investigations emerges noncommutative conditional independence in terms of a von Neumann algebraic structure closely related to Popa's notion of commuting squares and K\\"ummerer's generalized Bernoulli shifts. Our main result is applicable to classical probability, quantum probability, in particular free probability, braid group representations and Jones subfactors.

  5. Self-Gravitating Systems in Extended Gravity

    Directory of Open Access Journals (Sweden)

    Arturo Stabile

    2014-12-01

    Full Text Available Starting from the weak field limit, we discuss astrophysical applications of Extended Theories of Gravity where higher order curvature invariants and scalar fields are considered by generalizing the Hilbert-Einstein action linear in the Ricci curvature scalar R. Results are compared to General Relativity in the hypothesis that Dark Matter contributions to the dynamics can be neglected thanks to modified gravity. In particular, we consider stellar hydrostatic equilibrium, galactic rotation curves, and gravitational lensing. Finally, we discuss the weak field limit in the Jordan and Einstein frames pointing out how effective quantities, as gravitational potentials, transform from one frame to the other and the interpretation of results can completely change accordingly.

  6. ECO-EFFICIENCY WITHIN EXTENDED SUPPLY CHAIN

    Directory of Open Access Journals (Sweden)

    Handson Cláudio Dias Pimenta

    2012-05-01

    Full Text Available Designers make decisions that ultimately impact on both the economic and environmental performance of the products, and many of these costs and impacts occur across the supply chain. This paper proposes an initial discussion aboult eco-efficiency concepts applied within extended supply chain (ESC. Eco-efficiency (EE has the potential to incorporate both environmental and economic improvement by companies of ESC, and we explore the use of EE in the design process. It is noteworthy that it is an imperative in the current competitive market that companies must be able to manage their entire production chain taking into account environmental issues as an important factor in their decision-making processes. Therefore, it is believed that EE can integrate and strengthen a company’s functions and assist its decision-making processes as well as implement improvements within its ESC.

  7. Complete Solutions to Extended Stokes' Problems

    Directory of Open Access Journals (Sweden)

    Chi-Min Liu

    2008-01-01

    Full Text Available The main object of the present study is to theoretically solve the viscous flow of either a finite or infinite depth, which is driven by moving plane(s. Such a viscous flow is usually named as Stokes' first or second problems, which indicates the fluid motion driven by the impulsive or oscillating motion of the boundary, respectively. Traditional Stokes' problems are firstly revisited, and three extended problems are subsequently examined. Using some mathematical techniques and integral transforms, complete solutions which can exactly capture the flow characteristics at any time are derived. The corresponding steady-state and transient solutions are readily determined on the basis of complete solutions. Current results have wide applications in academic researches and are of significance for future studies taking more boundary conditions and non-Newtonian fluids into account.

  8. Extending OLAP Querying to External Object

    DEFF Research Database (Denmark)

    Pedersen, Torben Bach; Shoshani, Arie; Gu, Junmin

    inherent in data in nonstandard applications are not accommodated well by OLAP systems. In contrast, object database systems are built to handle such complexity, but do not support OLAP-type querying well. This paper presents the concepts and techniques underlying a flexible, multi-model federated system...... that enables OLAP users to exploit simultaneously the features of OLAP and object systems. The system allows data to be handled using the most appropriate data model and technology: OLAP systems for dimensional data and object database systems for more complex, general data. Additionally, physical data...... integration can be avoided. As a vehicle for demonstrating the capabilities of the system, a prototypical OLAP language is defined and extended to naturally support queries that involve data in object databases. The language permits selection criteria that reference object data, queries that return...

  9. Extended inertial range phenomenology of magnetohydrodynamic turbulence

    Science.gov (United States)

    Matthaeus, William H.; Zhou, YE

    1989-01-01

    A phenomenological treatment of the inertial range of isotropic statistically steady magnetohydrodynamic turbulence is presented, extending the theory of Kraichnan (1965). The role of Alfven wave propagation is treated on equal footing with nonlinear convection, leading to a simple generalization of the relations between the times characteristic of wave propagation, convection, energy transfer, and decay of triple correlations. The theory leads to a closed-form steady inertial range spectral law that reduces to the Kraichnan and Kolmogorov laws in appropriate limits. The Kraichnan constant is found to be related in a simple way to the Kolmogorov constant; for typical values of the latter constant, the former has values in the range 1.22-1.87. Estimates of the time scale associated with spectral transfer of energy also emerge from the new approach, generalizing previously presented 'golden rules' for relating the spectral transfer time scale to the Alfven and eddy-turnover time scales.

  10. Reasoning about Cardinal Directions between Extended Objects

    CERN Document Server

    Zhang, Xiaotong; Li, Sanjiang; Ying, Mingsheng

    2009-01-01

    Direction relations between extended spatial objects are important commonsense knowledge. Recently, Goyal and Egenhofer proposed a formal model, known as Cardinal Direction Calculus (CDC), for representing direction relations between connected plane regions. CDC is perhaps the most expressive qualitative calculus for directional information, and has attracted increasing interest from areas such as artificial intelligence, geographical information science, and image retrieval. Given a network of CDC constraints, the consistency problem is deciding if the network is realizable by connected regions in the real plane. This paper provides a cubic algorithm for checking consistency of basic CDC constraint networks, and proves that reasoning with CDC is in general an NP-Complete problem. For a consistent network of basic CDC constraints, our algorithm also returns a 'canonical' solution in cubic time. This cubic algorithm is also adapted to cope with cardinal directions between possibly disconnected regions, in whic...

  11. The Extended Relativity Theory in Clifford Spaces

    CERN Document Server

    Castro, C

    2004-01-01

    A brief review of some of the most important features of the Extended Relativity theory in Clifford-spaces ( $C$-spaces) is presented whose " point" coordinates are noncommuting Clifford-valued quantities and which incoporate the lines, areas, volumes, .... degrees of freedom associated with the collective particle, string, membrane, ... dynamics of the $p$-loop histories (closed p-branes) living in target $D$-dimensional spacetime backgrounds. $C$-space Relativity naturally incoporates the ideas of an invariant length (Planck scale), maximal acceleration, noncommuting coordinates, supersymmetry, holography, superluminal propagation, higher derivative gravity with torsion and variable dimensions/signatures that allows to study the dynamics of all (closed ) p-branes, for all values of $ p $, in a unified footing. It resolves the ordering ambiguities in QFT and the problem of time in Cosmology. A discussion of the maximal-acceleration Relativity principle in phase-spaces follows along with the study of the inva...

  12. The Extended Relativity Theory in Clifford Spaces

    CERN Document Server

    Castro, C

    2004-01-01

    A brief review of some of the most important features of the Extended Relativity theory in Clifford-spaces ($C$-spaces) is presented whose " point" coordinates are noncommuting Clifford-valued quantities and which incorporate the lines, areas, volumes,.... degrees of freedom associated with the collective particle, string, membrane,... dynamics of $p$-loops (closed p-branes) living in target $D$-dimensional spacetime backgrounds. $C$-space Relativity naturally incorporates the ideas of an invariant length (Planck scale), maximal acceleration, noncommuting coordinates, supersymmetry, holography, higher derivative gravity with torsion and variable dimensions/signatures that allows to study the dynamics of all (closed) p-branes, for all values of $ p $, on a unified footing. It resolves the ordering ambiguities in QFT and the problem of time in Cosmology. A discussion of the maximal-acceleration Relativity principle in phase-spaces follows along with the study of the invariance group of symmetry transformations ...

  13. VLA Polarimetry of Two Extended Radio Galaxies

    CERN Document Server

    Junor, W; Morganti, R; Padrielli, L

    2000-01-01

    Multi-wavelength VLA observations of two extended radio galaxies, 0235-197and 1203+043 are presented. There is some evidence from earlier studies thatthese two sources exhibit low frequency (<1 GHz) variability. This work showsthat both sources have linear polarizations, if any, below the detection limitsat 320 MHz, so we cannot explain the variability as being due to instrumentalpolarization effects as has been suggested for 3C159. Refractive scintillationmay be the cause of the variability in 0235-197. This would require theexistence of a bright, compact component in one of the hot spots seen in theseobservations. This is not implausible but the resolution of this observationalprogram is insufficent to address that question. The radio source 1203+043lacks any bright compact component thereby ruling out a refractivescintillation mechanism for its variability. Consequently, it is possible thatclaims of variability in this source are spurious. However, the 320 MHz VLAobservations show that 1203+043 has an `...

  14. Dynamic Visualization of Graphs with Extended Labels

    Energy Technology Data Exchange (ETDEWEB)

    Wong, Pak C.; Mackey, Patrick S.; Perrine, Kenneth A.; Eagan, James R.; Foote, Harlan P.; Thomas, Jim

    2005-10-23

    The paper describes a novel technique to visualize graphs with extended node and link labels. The lengths of these labels range from a short phrase to a full sentence to an entire paragraph and beyond. Our solution is different from all the existing approaches that almost always rely on intensive computational effort to optimize the label placement problem. Instead, we share the visualization resources with the graph and present the label information in static, interactive, and dynamic modes without the requirement for tackling the intractability issues. This allows us to reallocate the computational resources for dynamic presentation of real-time information. The paper includes a user study to evaluate the effectiveness and efficiency of the visualization technique.

  15. Extended Analysis of the Casimir Force

    Directory of Open Access Journals (Sweden)

    Lehnert B.

    2014-04-01

    Full Text Available There are several arguments for the conventional form of the Zero Point Energy fre- quency spectrum to be put in doubt. It has thus to be revised in to that of a self-consistent system in statistical equilibrium where the total energy de nsity and the equivalent pres- sure become finite. An extended form of the Casimir force is th ereby proposed to be used as a tool for determining the local magnitude of the same pressure. This can be done in terms of measurements on the force between a pair po lished plane plates consisting of different metals, the plates having very small or zero air gaps. T his corre- sponds to the largest possible Casimir force. Even then, the re may arise problems with other adhering forces, possibly to be clarified in further experiments.

  16. Extended constitutive laws for lamellar phases

    Directory of Open Access Journals (Sweden)

    Chi-Deuk Yoo

    2013-10-01

    Full Text Available Classically, stress and strain rate in linear viscoelastic materials are related by a constitutive relationship involving the viscoelastic modulus G(t. The same constitutive law, within Linear Response Theory, relates currents of conserved quantities and gradients of existing conjugate variables, and it involves the autocorrelation functions of the currents in equilibrium. We explore the consequences of the latter relationship in the case of a mesoscale model of a block copolymer, and derive the resulting relationship between viscous friction and order parameter diffusion that would result in a lamellar phase. We also explicitly consider in our derivation the fact that the dissipative part of the stress tensor must be consistent with the uniaxial symmetry of the phase. We then obtain a relationship between the stress and order parameter autocorrelation functions that can be interpreted as an extended constitutive law, one that offers a way to determine them from microscopic experiment or numerical simulation.

  17. Cosmological Perturbations in Extended Massive Gravity

    CERN Document Server

    Gumrukcuoglu, A Emir; Lin, Chunshan; Mukohyama, Shinji; Trodden, Mark

    2013-01-01

    We study cosmological perturbations around self-accelerating solutions to two extensions of nonlinear massive gravity: the quasi-dilaton theory and the mass-varying theory. We examine stability of the cosmological solutions, and the extent to which the vanishing of the kinetic terms for scalar and vector perturbations of self-accelerating solutions in massive gravity is generic when the theory is extended. We find that these kinetic terms are in general non-vanishing in both extensions, though there are constraints on the parameters and background evolution from demanding that they have the correct sign. In particular, the self-accelerating solutions of the quasi-dilaton theory are always unstable to scalar perturbations with wavelength shorter than the Hubble length.

  18. Extended SQL for manipulating clinical warehouse data.

    Science.gov (United States)

    Johnson, S B; Chatziantoniou, D

    1999-01-01

    Health care institutions are beginning to collect large amounts of clinical data through patient care applications. Clinical data warehouses make these data available for complex analysis across patient records, benefiting administrative reporting, patient care and clinical research. Data gathered for patient care purposes are difficult to manipulate for analytic tasks; the schema presents conceptual difficulties for the analyst, and many queries perform poorly. An extension to SQL is presented that enables the analyst to designate groups of rows. These groups can then be manipulated and aggregated in various ways to solve a number of useful analytic problems. The extended SQL is concise and runs in linear time, while standard SQL requires multiple statements with polynomial performance. The extensions are extremely powerful for performing aggregations on large amounts of data, which is useful in clinical data mining applications.

  19. Descriptive complexity for pictures languages (extended abstract)

    CERN Document Server

    Grandjean, Etienne; richard, Gaétan

    2012-01-01

    This paper deals with descriptive complexity of picture languages of any dimension by syntactical fragments of existential second-order logic. - We uniformly generalize to any dimension the characterization by Giammarresi et al. \\cite{GRST96} of the class of \\emph{recognizable} picture languages in existential monadic second-order logic. - We state several logical characterizations of the class of picture languages recognized in linear time on nondeterministic cellular automata of any dimension. They are the first machine-independent characterizations of complexity classes of cellular automata. Our characterizations are essentially deduced from normalization results we prove for first-order and existential second-order logics over pictures. They are obtained in a general and uniform framework that allows to extend them to other "regular" structures. Finally, we describe some hierarchy results that show the optimality of our logical characterizations and delineate their limits.

  20. Neutrino Anomalies in an Extended Zee Model

    CERN Document Server

    Joshipura, A S; Joshipura, Anjan S.; Rindani, Saurabh D.

    1999-01-01

    We discuss an extended SU(2)X U(1) model which naturally leads to mass scales and mixing angles relevant for understanding both the solar and atmospheric neutrino anomalies. No right-handed neutrinos are introduced in the model.The model uses a softly broken L_e-L_{\\mu}-L_{\\tau} symmetry. Neutrino masses arise only at the loop level. The one-loop neutrino masses which arise as in the Zee model solve the atmospheric neutrino anomaly while breaking of L_e-L_{\\mu}-L_{\\tau} generates at two-loop order a mass splitting needed for the vacuum solution of the solar neutrino problem. A somewhat different model is possible which accommodates the large-angle MSW resolution of the solar neutrino problem.

  1. Observational constraints on extended Chaplygin gas cosmologies

    Indian Academy of Sciences (India)

    B C PAUL; P THAKUR; A SAHA

    2017-08-01

    We investigate cosmological models with extended Chaplygin gas (ECG) as a candidate for dark energy and determine the equation of state parameters using observed data namely, observed Hubble data, baryon acousticoscillation data and cosmic microwave background shift data. Cosmological models are investigated considering cosmic fluid which is an extension of Chaplygin gas, however, it reduces to modified Chaplygin gas (MCG) andalso to generalized Chaplygin gas (GCG) in special cases. It is found that in the case of MCG and GCG, the best-fit values of all the parameters are positive. The distance modulus agrees quite well with the experimental Union2data. The speed of sound obtained in the model is small, necessary for structure formation. We also determine the observational constraints on the constants of the ECG equation.

  2. Extended Higgs sectors in radiative neutrino models

    Directory of Open Access Journals (Sweden)

    Oleg Antipin

    2017-05-01

    Full Text Available Testable Higgs partners may be sought within the extensions of the SM Higgs sector aimed at generating neutrino masses at the loop level. We study a viability of extended Higgs sectors for two selected models of radiative neutrino masses: a one-loop mass model, providing the Higgs partner within a real triplet scalar representation, and a three-loop mass model, providing it within its two-Higgs-doublet sector. The Higgs sector in the one-loop model may remain stable and perturbative up to the Planck scale, whereas the three-loop model calls for a UV completion around 106 GeV. Additional vector-like lepton and exotic scalar fields, which are required to close one- and three-loop neutrino-mass diagrams, play a decisive role for the testability of the respective models. We constrain the parameter space of these models using LHC bounds on diboson resonances.

  3. Extending stochastic network calculus to loss analysis.

    Science.gov (United States)

    Luo, Chao; Yu, Li; Zheng, Jun

    2013-01-01

    Loss is an important parameter of Quality of Service (QoS). Though stochastic network calculus is a very useful tool for performance evaluation of computer networks, existing studies on stochastic service guarantees mainly focused on the delay and backlog. Some efforts have been made to analyse loss by deterministic network calculus, but there are few results to extend stochastic network calculus for loss analysis. In this paper, we introduce a new parameter named loss factor into stochastic network calculus and then derive the loss bound through the existing arrival curve and service curve via this parameter. We then prove that our result is suitable for the networks with multiple input flows. Simulations show the impact of buffer size, arrival traffic, and service on the loss factor.

  4. The extended Estabrook-Wahlquist method

    Science.gov (United States)

    Choudhury, S. Roy; Russo, Matthew

    2016-07-01

    Variable Coefficient Korteweg de Vries (vcKdV), modified Korteweg de Vries (vcMKdV), and nonlinear Schrödinger (NLS) equations have a long history dating from their derivation in various applications. A technique based on extended Lax Pairs has been devised recently to derive time-and-space-dependent-coefficient generalizations of various such Lax-integrable NLPDE hierarchies, which are thus more general than almost all cases considered earlier via methods such as the Painlevé Test, Bell Polynomials, and similarity methods. However, this technique, although operationally effective, has the significant disadvantage that, for any integrable system with spatiotemporally varying coefficients, one must 'guess' a generalization of the structure of the known Lax Pair for the corresponding system with constant coefficients. Motivated by the somewhat arbitrary nature of the above procedure, we embark in this paper on an attempt to systematize the derivation of Lax-integrable systems with variable coefficients. We consider the Estabrook-Wahlquist (EW) prolongation technique, a relatively self-consistent procedure requiring little prior information. However, this immediately requires that the technique be significantly generalized in several ways, including solving matrix partial differential equations instead of algebraic ones as the structure of the Lax Pair is systematically computed, and also in solving the constraint equations to deduce the explicit forms for various 'coefficient' matrices. The new and extended EW technique which results is illustrated by algorithmically deriving generalized Lax-integrable versions of the NLS, generalized fifth-order KdV, MKdV, and derivative nonlinear Schrödinger (DNLS) equations. We also show how this method correctly excludes the existence of a nontrivial Lax pair for a nonintegrable NLPDE such as the variable-coefficient cubic-quintic NLS.

  5. Beyond six parameters: Extending Λ CDM

    Science.gov (United States)

    Di Valentino, Eleonora; Melchiorri, Alessandro; Silk, Joseph

    2015-12-01

    Cosmological constraints are usually derived under the assumption of a six-parameter Λ CDM theoretical framework or simple one-parameter extensions. In this paper we present, for the first time, cosmological constraints in a significantly extended scenario, varying up to 12 cosmological parameters simultaneously, including the sum of neutrino masses, the neutrino effective number, the dark energy equation of state, the gravitational wave background and the running of the spectral index of primordial perturbations. Using the latest Planck 2015 data release (with polarization), we found no significant indication for extensions to the standard Λ CDM scenario, with the notable exception of the angular power spectrum lensing amplitude, Alens , which is larger than the expected value at more than 2 standard deviations, even when combining the Planck data with BAO and supernovae type Ia external data sets. In our extended cosmological framework, we find that a combined Planck+BAO analysis constrains the value of the rms density fluctuation parameter to σ8=0.781-0.063+0.065 at 95 % C.L., helping to relieve the possible tensions with the CFHTlenS cosmic shear survey. We also find a lower value for the reionization optical depth τ =0.058-0.043+0.040 at 95 % C.L. with respect to the one derived under the assumption of Λ CDM . The scalar spectral index nS is now compatible with a Harrison-Zeldovich spectrum to within 2.5 standard deviations. Combining the Planck data set with the Hubble Space Telescope prior on the Hubble constant provides a value for the equation of state w <-1 at more than 2 standard deviations, while the neutrino effective number is fully compatible with the expectations of the standard three neutrino framework.

  6. The Extended Relativity Theory in Clifford Spaces

    Directory of Open Access Journals (Sweden)

    Castro C.

    2005-04-01

    Full Text Available An introduction to some of the most important features of the Extended Relativity theory in Clifford-spaces (C-spaces is presented whose “point” coordinates are non-commuting Clifford-valued quantities which incorporate lines, areas, volumes, hyper-volumes. . . degrees of freedom associated with the collective particle, string, membrane, p-brane. . . dynamics of p-loops (closed p-branes in target D-dimensional spacetime backgrounds. C-space Relativity naturally incorporates the ideas of an invariant length (Planck scale, maximal acceleration, non-commuting coordinates, supersymmetry, holography, higher derivative gravity with torsion and variable dimensions/signatures. It permits to study the dynamics of all (closed p-branes, for all values of p, on a unified footing. It resolves the ordering ambiguities in QFT, the problem of time in Cosmology and admits superluminal propagation (tachyons without violations of causality. A discussion of the maximal-acceleration Relativity principle in phase-spaces follows and the study of the invariance group of symmetry transformations in phase-space allows to show why Planck areas are invariant under acceleration-boosts transformations. This invariance feature suggests that a maximal-string tension principle may be operating in Nature. We continue by pointing out how the relativity of signatures of the underlying n-dimensional spacetime results from taking different n-dimensional slices through C-space. The conformal group in spacetime emerges as a natural subgroup of the Clifford group and Relativity in C-spaces involves natural scale changes in the sizes of physical objects without the introduction of forces nor Weyl’s gauge field of dilations. We finalize by constructing the generalization of Maxwell theory of Electrodynamics of point charges to a theory in C-spaces that involves extended charges coupled to antisymmetric tensor fields of arbitrary rank. In the concluding remarks we outline briefly

  7. Hyperuniformity disorder length spectroscopy for extended particles

    Science.gov (United States)

    Durian, D. J.

    2017-09-01

    The concept of a hyperuniformity disorder length h was recently introduced for analyzing volume fraction fluctuations for a set of measuring windows [Chieco et al., Phys. Rev. E 96, 032909 (2017)., 10.1103/PhysRevE.96.032909]. This length permits a direct connection to the nature of disorder in the spatial configuration of the particles and provides a way to diagnose the degree of hyperuniformity in terms of the scaling of h and its value in comparison with established bounds. Here, this approach is generalized for extended particles, which are larger than the image resolution and can lie partially inside and partially outside the measuring windows. The starting point is an expression for the relative volume fraction variance in terms of four distinct volumes: that of the particle, the measuring window, the mean-squared overlap between particle and region, and the region over which particles have nonzero overlap with the measuring window. After establishing limiting behaviors for the relative variance, computational methods are developed for both continuum and pixelated particles. Exact results are presented for particles of special shape and for measuring windows of special shape, for which the equations are tractable. Comparison is made for other particle shapes, using simulated Poisson patterns. And the effects of polydispersity and image errors are discussed. For small measuring windows, both particle shape and spatial arrangement affect the form of the variance. For large regions, the variance scaling depends only on arrangement but particle shape sets the numerical proportionality. The combined understanding permit the measured variance to be translated to the spectrum of hyperuniformity lengths versus region size, as the quantifier of spatial arrangement. This program is demonstrated for a system of nonoverlapping particles at a series of increasing packing fractions as well as for an Einstein pattern of particles with several different extended shapes.

  8. Extended mind and after: socially extended mind and actor-network.

    Science.gov (United States)

    Kono, Tetsuya

    2014-03-01

    The concept of extended mind has been impressively developed over the last 10 years by many philosophers and cognitive scientists. The extended mind thesis (EM) affirms that the mind is not simply ensconced inside the head, but extends to the whole system of brain-body-environment. Recently, some philosophers and psychologists try to adapt the idea of EM to the domain of social cognition research. Mind is socially extended (SEM). However, EM/SEM theory has problems to analyze the interactions among a subject and its surroundings with opposition, antagonism, or conflict; it also tends to think that the environment surrounding the subject is passive or static, and to neglect the power of non-human actants to direct and regulate the human subject. In these points, actor-network theory (ANT) proposed by Latour and Callon is more persuasive, while sharing some important ideas with EM/SEM theory. Actor-network is a hybrid community which is composed of a series of heterogeneous elements, animate and inanimate for a certain period of time. I shall conclude that EM/SEM could be best analyzed as a special case of actor-network. EM/SEM is a system which can be controlled by a human agent alone. In order to understand collective behavior, philosophy and psychology have to study the actor-network in which human individuals are situated.

  9. Planning of the Extended Reach well Dieksand 2; Planung der Extended Reach Bohrung Dieksand 2

    Energy Technology Data Exchange (ETDEWEB)

    Frank, U.; Berners, H. [RWE-DEA AG, Hamburg (Germany). Drilling Team Mittelplate und Dieksand; Hadow, A.; Klop, G.; Sickinger, W. [Wintershall AG Erdoelwerke, Barnstdorf (Germany); Sudron, K.

    1998-12-31

    The Mittelplate oil field is located 7 km offshore the town of Friedrichskoog. Reserves are estimated at 30 million tonnes of oil. At a production rate of 2,500 t/d, it will last about 33 years. The transport capacity of the offshore platform is limited, so that attempts were made to enhance production by constructing the extended reach borehole Dieksand 2. Details are presented. (orig.) [Deutsch] Das Erdoelfeld Mittelplate liegt am suedlichen Rand des Nationalparks Schleswig Holsteinisches Wattenmeer, ca. 7000 m westlich der Ortschaft Friedrichskoog. Die gewinnbaren Reserven betragen ca. 30 Millionen t Oel. Bei einer Foerderkapazitaet von 2.500 t/Tag betraegt die Foerderdauer ca. 33 Jahre. Aufgrund der begrenzten Transportkapazitaeten von der Insel, laesst sich durch zusaetzliche Bohrungen von der kuenstlichen Insel Mittelplate keine entscheidende Erhoehung der Foerderkapazitaet erzielen. Ab Sommer 1996 wurde erstmals die Moeglichkeit der Lagerstaettenerschliessung von Land untersucht. Ein im Mai 1997 in Hamburg etabliertes Drilling Team wurde mit der Aufgabe betraut, die Extended Reach Bohrung Dieksand 2 zu planen und abzuteufen. Die Planungsphasen fuer die Extended Reach Bohrung Dieksand 2 wurden aufgezeigt. Die fuer den Erfolg einer Extended Reach Bohrung wichtigen Planungsparameter wurden erlaeutert. Es wurden Wege gezeigt, wie bei diesem Projekt technische und geologische Risiken in der Planung mit beruecksichtigt und nach Beginn der Bohrung weiter bearbeitet werden koennen. (orig.)

  10. A Formalization and Proof of the Extended Church-Turing Thesis -Extended Abstract-

    Directory of Open Access Journals (Sweden)

    Nachum Dershowitz

    2012-07-01

    Full Text Available We prove the Extended Church-Turing Thesis: Every effective algorithm can be efficiently simulated by a Turing machine. This is accomplished by emulating an effective algorithm via an abstract state machine, and simulating such an abstract state machine by a random access machine, representing data as a minimal term graph.

  11. Should nucleotide sequence analyzing computer algorithms always extend homologies by extending homologies?

    Science.gov (United States)

    Burnett, L; Basten, A; Hensley, W J

    1986-01-10

    Most computer algorithms used for comparing or aligning nucleotide sequences rely on the premise that the best way to extend a homology between the two sequences is to select a match rather than a mismatch. We have tested this assumption and found that it is not always valid.

  12. Extended Deterrence, Nuclear Proliferation, and START III

    Energy Technology Data Exchange (ETDEWEB)

    Speed, R.D.

    2000-06-20

    Early in the Cold War, the United States adopted a policy of ''extended nuclear deterrence'' to protect its allies by threatening a nuclear strike against any state that attacks these allies. This threat can (in principle) be used to try to deter an enemy attack using conventional weapons or one using nuclear, chemical, or biological weapons. The credibility of a nuclear threat has long been subject to debate and is dependent on many complex geopolitical factors, not the least of which is the military capabilities of the opposing sides. The ending of the Cold War has led to a significant decrease in the number of strategic nuclear weapons deployed by the United States and Russia. START II, which was recently ratified by the Russian Duma, will (if implemented) reduce the number deployed strategic nuclear weapons on each side to 3500, compared to a level of over 11,000 at the end of the Cold War in 1991. The tentative limit established by Presidents Clinton and Yeltsin for START III would reduce the strategic force level to 2000-2500. However, the Russians (along with a number of arms control advocates) now argue that the level should be reduced even further--to 1500 warheads or less. The conventional view is that ''deep cuts'' in nuclear weapons are necessary to discourage nuclear proliferation. Thus, as part of the bargain to get the non-nuclear states to agree to the renewal of the Nuclear Non-Proliferation Treaty, the United States pledged to work towards greater reductions in strategic forces. Without movement in the direction of deep cuts, it is thought by many analysts that some countries may decide to build their own nuclear weapons. Indeed, this was part of the rationale India used to justify its own nuclear weapons program. However, there is also some concern that deep cuts (to 1500 or lower) in the U.S. strategic nuclear arsenal could have the opposite effect. The fear is that such cuts might undermine extended

  13. Extended Forward Sensitivity Analysis for Uncertainty Quantification

    Energy Technology Data Exchange (ETDEWEB)

    Haihua Zhao; Vincent A. Mousseau

    2011-09-01

    Verification and validation (V&V) are playing more important roles to quantify uncertainties and realize high fidelity simulations in engineering system analyses, such as transients happened in a complex nuclear reactor system. Traditional V&V in the reactor system analysis focused more on the validation part or did not differentiate verification and validation. The traditional approach to uncertainty quantification is based on a 'black box' approach. The simulation tool is treated as an unknown signal generator, a distribution of inputs according to assumed probability density functions is sent in and the distribution of the outputs is measured and correlated back to the original input distribution. The 'black box' method mixes numerical errors with all other uncertainties. It is also not efficient to perform sensitivity analysis. Contrary to the 'black box' method, a more efficient sensitivity approach can take advantage of intimate knowledge of the simulation code. In these types of approaches equations for the propagation of uncertainty are constructed and the sensitivities are directly solved for as variables in the simulation. This paper presents the forward sensitivity analysis as a method to help uncertainty qualification. By including time step and potentially spatial step as special sensitivity parameters, the forward sensitivity method is extended as one method to quantify numerical errors. Note that by integrating local truncation errors over the whole system through the forward sensitivity analysis process, the generated time step and spatial step sensitivity information reflect global numerical errors. The discretization errors can be systematically compared against uncertainties due to other physical parameters. This extension makes the forward sensitivity method a much more powerful tool to help uncertainty qualification. By knowing the relative sensitivity of time and space steps with other interested physical

  14. An Extended Enterprise Modeling Approach to Enterprise-based Integration

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The paradigm of extended enterprise is the core competency focused. An extended enterprise expands its scope from bounding a single enterprise to including additional processes performed by other enterprises. The integration of processes is enterprise based. This paper proposes a recursive enterprises interconnected chain model for the extended enterprise, and presents an enterprise-based integration framework for the extended enterprise. The case study is based on a motorcycle group corporation.

  15. Thermalization and Pseudolocality in Extended Quantum Systems

    Science.gov (United States)

    Doyon, Benjamin

    2017-04-01

    Recently, it was understood that modified concepts of locality played an important role in the study of extended quantum systems out of equilibrium, in particular in so-called generalized Gibbs ensembles. In this paper, we rigorously study pseudolocal charges and their involvement in time evolutions and in the thermalization process of arbitrary states with strong enough clustering properties. We show that the densities of pseudolocal charges form a Hilbert space, with inner product determined by thermodynamic susceptibilities. Using this, we define the family of pseudolocal states, which are determined by pseudolocal charges. This family includes thermal Gibbs states at high enough temperatures, as well as (a precise definition of) generalized Gibbs ensembles. We prove that the family of pseudolocal states is preserved by finite time evolution, and that, under certain conditions, the stationary state emerging at infinite time is a generalized Gibbs ensemble with respect to the evolution dynamics. If the evolution dynamics does not admit any conserved pseudolocal charges other than the evolution Hamiltonian, we show that any stationary pseudolocal state with respect to these dynamics is a thermal Gibbs state, and that Gibbs thermalization occurs. The framework is that of translation-invariant states on hypercubic quantum lattices of any dimensionality (including quantum chains) and finite-range Hamiltonians, and does not involve integrability.

  16. Positive mass theorem in extended supergravities

    Directory of Open Access Journals (Sweden)

    Masato Nozawa

    2014-10-01

    Full Text Available Following the Witten–Nester formalism, we present a useful prescription using Weyl spinors towards the positivity of mass. As a generalization of arXiv:1310.1663, we show that some “positivity conditions” must be imposed upon the gauge connections appearing in the supercovariant derivative acting on spinors. A complete classification of the connection fulfilling the positivity conditions is given. It turns out that these positivity conditions are indeed satisfied for a number of extended supergravity theories. It is shown that the positivity property holds for the Einstein-complex scalar system, provided that the target space is Hodge–Kähler and the potential is expressed in terms of the superpotential. In the Einstein–Maxwell-dilaton theory with a dilaton potential, the dilaton coupling function and the superpotential are fixed by the positive mass property. We also explore the N=8 gauged supergravity and demonstrate that the positivity of the mass holds independently of the gaugings and the deformation parameters.

  17. Conceptual Framework To Extend Life Cycle Assessment ...

    Science.gov (United States)

    Life Cycle Assessment (LCA) is a decision-making tool that accounts for multiple impacts across the life cycle of a product or service. This paper presents a conceptual framework to integrate human health impact assessment with risk screening approaches to extend LCA to include near-field chemical sources (e.g., those originating from consumer products and building materials) that have traditionally been excluded from LCA. A new generation of rapid human exposure modeling and high-throughput toxicity testing is transforming chemical risk prioritization and provides an opportunity for integration of screening-level risk assessment (RA) with LCA. The combined LCA and RA approach considers environmental impacts of products alongside risks to human health, which is consistent with regulatory frameworks addressing RA within a sustainability mindset. A case study is presented to juxtapose LCA and risk screening approaches for a chemical used in a consumer product. The case study demonstrates how these new risk screening tools can be used to inform toxicity impact estimates in LCA and highlights needs for future research. The framework provides a basis for developing tools and methods to support decision making on the use of chemicals in products. This paper presents a conceptual framework for including near-field exposures into Life Cycle Assessment using advanced human exposure modeling and high-throughput tools

  18. Remineralization of caries lesions extending into dentin.

    Science.gov (United States)

    ten Cate, J M

    2001-05-01

    Remineralization is one aspect of the overall process of tooth decay. However, it is primarily studied in shallow lesions. The aim of this study was to explore whether caries lesions in enamel and extending into the dentin can be remineralized. A single-section model was developed for the longitudinal and non-destructive monitoring of changes in enamel and dentin. Lesions at least 200 microm into dentin were formed in undersaturated acetate buffers. Next, the lesions were divided into groups (three treatment and one control) and remineralized. The treatments were: weekly immersion in 1,000 ppm fluoride, single treatment with methanehydroxybisphosphonate, and a constant level of 1 ppm fluoride. De- and remineralization was assessed by transverse microradiography. Remineralization was observed in enamel, but also in dentin, indicating that, deep into dentin, the pores become supersaturated to apatite formation. Treatments affected remineralization only in the outer part of enamel. Both findings are explained by a relatively fast diffusion of mineral ions, with precipitation being rate-limiting. The results suggest that dentin remineralization, underneath enamel, can be achieved and could possibly be used in clinical treatment strategies.

  19. Extending particle tracking capability with Delaunay triangulation.

    Science.gov (United States)

    Chen, Kejia; Anthony, Stephen M; Granick, Steve

    2014-04-29

    Particle tracking, the analysis of individual moving elements in time series of microscopic images, enables burgeoning new applications, but there is need to better resolve conformation and dynamics. Here we describe the advantages of Delaunay triangulation to extend the capabilities of particle tracking in three areas: (1) discriminating irregularly shaped objects, which allows one to track items other than point features; (2) combining time and space to better connect missing frames in trajectories; and (3) identifying shape backbone. To demonstrate the method, specific examples are given, involving analyzing the time-dependent molecular conformations of actin filaments and λ-DNA. The main limitation of this method, shared by all other clustering techniques, is the difficulty to separate objects when they are very close. This can be mitigated by inspecting locally to remove edges that are longer than their neighbors and also edges that link two objects, using methods described here, so that the combination of Delaunay triangulation with edge removal can be robustly applied to processing large data sets. As common software packages, both commercial and open source, can construct Delaunay triangulation on command, the methods described in this paper are both computationally efficient and easy to implement.

  20. Module theory, extending modules and generalizations

    CERN Document Server

    Tercan, Adnan

    2016-01-01

    The main focus of this monograph is to offer a comprehensive presentation of known and new results on various generalizations of CS-modules and CS-rings. Extending (or CS) modules are generalizations of injective (and also semisimple or uniform) modules. While the theory of CS-modules is well documented in monographs and textbooks, results on generalized forms of the CS property as well as dual notions are far less present in the literature. With their work the authors provide a solid background to module theory, accessible to anyone familiar with basic abstract algebra. The focus of the book is on direct sums of CS-modules and classes of modules related to CS-modules, such as relative (injective) ejective modules, (quasi) continuous modules, and lifting modules. In particular, matrix CS-rings are studied and clear proofs of fundamental decomposition results on CS-modules over commutative domains are given, thus complementing existing monographs in this area. Open problems round out the work and establish the...

  1. ANALYSING SPACE: ADAPTING AND EXTENDING MULTIMODAL SEMIOTICS

    Directory of Open Access Journals (Sweden)

    Louise J. Ravelli

    2015-07-01

    Full Text Available In the field of multimodal discourse analysis, one of the most exciting sites of application is that of 3D space: examining aspects of built environment for its meaningmaking potential. For the built environment – homes, offices, public buildings, parks, etc. – does indeed make meaning. These are spaces which speak – often their meanings are so familiar, we no longer hear what they say; sometimes, new and unusual sites draw attention to their meanings, and they are hotly contested. This chapter will suggest ways of analyzing 3D texts, based on the framework of Kress and van Leeuwen (2006. This framework, developed primarily for the analysis of 2D images, has been successfully extended to a range of other multimodal texts. Extension to the built environment includes Pang (2004, O’Toole (1994, Ravelli (2006, Safeyton (2004, Stenglin (2004 and White (1994, whose studies will inform the analyses presented here. This article will identify some of the key theoretical principles which underline this approach, including the notions of text, context and metafunction, and will describe some of the main areas of analysis for 3D texts. Also, ways of bringing the analyses together will be considered. The analyses will be demonstrated in relation to the Scientia building at the University of New South Wales, Australia.

  2. Extended Lorentz code of a superluminal particle

    CERN Document Server

    Ter-Kazarian, G

    2012-01-01

    While the OPERA experimental scrutiny is ongoing in the community, in the present article we construct a toy model of {\\it extended Lorentz code} (ELC) of the uniform motion, which will be a well established consistent and unique theoretical framework to explain the apparent violations of the standard Lorentz code (SLC), the possible manifestations of which arise in a similar way in all particle sectors. We argue that in the ELC-framework the propagation of the superluminal particle, which implies the modified dispersion relation, could be consistent with causality. Furthermore, in this framework, we give a justification of forbiddance of Vavilov-Cherenkov (VC)-radiation/or analog processes in vacuum. To be consistent with the SN1987A and OPERA data, we identify the neutrinos from SN1987A and the light as so-called {\\it 1-th type} particles carrying the {\\it individual Lorentz motion code} with the velocity of light $c_{1}\\equiv c$ in vacuum as maximum attainable velocity for all the 1-th type particles. Ther...

  3. Extended Particles and the Exterior Calculus

    CERN Document Server

    Tucker, R W

    2016-01-01

    These notes were delivered as a series of NIMROD lectures at the Rutherford Appleton Laboratory by the author in February 1976 (RL-76-022). The purpose of these lectures was primarily two-fold: to discuss the classical theory of free point particles, free strings and free membranes from a unified viewpoint; and to present in the process of doing this the rudiments of an intrinsic geometrical calculus that the author has found of immense value in investigating these systems. It is shown how the equations of motion for such classically extended relativistic systems arise in a very simple manner from a principle of stationary action and furthermore how the boundary conditions for finite systems may be derived in a gauge invariant way. Momenta are naturally introduced and the primary constraints that exist in a Hamiltonian description follow simply. Calculations may proceed in an index-free manner until components are required. It is at this stage that one can, if one desires, impose gauge conditions and remove n...

  4. Extending a context model for microphone forensics

    Science.gov (United States)

    Kraetzer, Christian; Qian, Kun; Dittmann, Jana

    2012-03-01

    In this paper, we extend an existing context model for statistical pattern recognition based microphone forensics by: first, generating a generalized model for this process and second, using this general model to construct a complex new application scenario model for microphone forensic investigations on the detection of playback recordings (a.k.a. replays, re-recordings, double-recordings). Thereby, we build the theoretical basis for answering the question whether an audio recording was made to record a playback or natural sound. The results of our investigations on the research question of playback detection imply that it is possible with our approach on our evaluation set of six microphones. If the recorded sound is not modified prior to playback, we achieve in our tests 89.00% positive indications on the correct two microphones involved. If the sound is post-processed (here, by normalization) this figure decreases (in our normalization example to 36.00%, while another 50.67% of the tests still indicate two microphones, of which one has actually not been involved in the recording and playback recording process).

  5. An Extended View of the Fornax Cluster

    CERN Document Server

    Waugh, M; Drinkwater, M J

    2000-01-01

    The Multibeam survey at the Parkes Radio Telescope has provided a wealth of 21 cm HI data. We have mosaicked ten standard HIPASS cubes to produce a datacube approximately 25 deg x 25 deg in size, centred on NGC1399, the optical centre of the Fornax Cluster. Some properties of the initial approx. 80 galaxies identified in HI are described. In the optical, Ferguson's Fornax Cluster Catalogue (FCC) identified 340 likely member galaxies in the central 40 sq.deg. In the radio we have detected a more uniform sheet of about 80 galaxies at the cluster velocity extending up to 15 deg from the cluster centre. At the cluster distance of about 15 Mpc, this corresponds to an elongated large-scale structure (LSS) more than 7 Mpc in extent. Galaxies were detected to a lower mass limit of 1x10^8M_sun and fewer than 25 of these were within the central 40 sq.deg, suggesting considerable HI depletion of galaxies in the centre of the cluster. Further, these results strongly indicate that HI surveys do not readily identify galaxy...

  6. Extended force density method and its expressions

    CERN Document Server

    Miki, Masaaki

    2011-01-01

    The objective of this work can be divided into two parts. The first one is to propose an extension of the force density method (FDM)(H.J. Schek, 1974), a form-finding method for prestressed cable-net structures. The second one is to present a review of various form-finding methods for tension structures, in the relation with the extended FDM. In the first part, it is pointed out that the original FDM become useless when it is applied to the prestressed structures that consist of combinations of both tension and compression members, while the FDM is usually advantageous in form-finding analysis of cable-nets. To eliminate the limitation, a functional whose stationary problem simply represents the FDM is firstly proposed. Additionally, the existence of a variational principle in the FDM is also indicated. Then, the FDM is extensively redefined by generalizing the formulation of the functional. As the result, the generalized functionals enable us to find the forms of tension structures that consist of combinatio...

  7. An extended database of keratin binding.

    Science.gov (United States)

    Hansen, Steffi; Selzer, Dominik; Schaefer, Ulrich F; Kasting, Gerald B

    2011-05-01

    Diffusion modeling of dermal absorption relies in large part on high quality input data. Currently, estimates of corneocyte-phase partitioning are based on an analysis of a dataset of limited size and diversity. Therefore, we have updated and broadened the analysis. For this purpose, binding coefficients to different keratins, namely, bovine hoof and horn, human delipidized callus, human delipidized stratum corneum (SC), human nail, human hair, and sheep wool were collected from the literature. In addition, binding coefficients to hoof/horn and delipidized SC were measured for eight hydrophilic compounds including three ionizable compounds that were measured at different pH values. Important results are: (i) only hoof/horn, callus, and delipidized SC are suitable keratins for estimating corneocyte protein binding; (ii) binding coefficients to hoof/horn, callus, and delipidized SC can be predicted from the octanol-water partition coefficients log K(o/w) confirming the analysis of the limited dataset; (iii) binding of ionizable compounds can be predicted by correcting log K(o/w) for pH; (iv) the correlation derived for the extended database is steeper than the relationship derived for the limited dataset. This has consequences for the estimates of SC partition and diffusion coefficients for diffusion modeling of dermal absorption. .

  8. Evidence for an extended scattered disk

    CERN Document Server

    Gladman, B; Grav, T; Kavelaars, J J; Nicholson, P; Aksnes, K; Petit, J M

    2001-01-01

    By telescopic tracking, we have established that the orbit of the trans-neptunian object (2000 CR$_{105}$) has a perihelion of $\\simeq$44 AU, and is thus outside the domain controlled by strong gravitational close encounters with Neptune. Because this object is on a very large, eccentric orbit (with semimajor axis $a\\simeq$216 AU and eccentricity $e\\simeq$0.8) this object must have been placed on this orbit by a gravitational perturbation which is {\\it not} direct gravitational scattering off of any of the giant planets (on their current orbits). The existence of this object may thus have profound cosmogonic implications for our understanding of the formation of the outer Solar System. We discuss some viable scenarios which could have produced it, including long-term diffusive chaos and scattering off of other massive bodies in the outer Solar System. This discovery implies that there must be a large population of trans-neptunian objects in an `extended scattered disk' with perihelia above the previously-disc...

  9. Enhanced Graphics for Extended Scale Range

    Science.gov (United States)

    Hanson, Andrew J.; Chi-Wing Fu, Philip

    2012-01-01

    Enhanced Graphics for Extended Scale Range is a computer program for rendering fly-through views of scene models that include visible objects differing in size by large orders of magnitude. An example would be a scene showing a person in a park at night with the moon, stars, and galaxies in the background sky. Prior graphical computer programs exhibit arithmetic and other anomalies when rendering scenes containing objects that differ enormously in scale and distance from the viewer. The present program dynamically repartitions distance scales of objects in a scene during rendering to eliminate almost all such anomalies in a way compatible with implementation in other software and in hardware accelerators. By assigning depth ranges correspond ing to rendering precision requirements, either automatically or under program control, this program spaces out object scales to match the precision requirements of the rendering arithmetic. This action includes an intelligent partition of the depth buffer ranges to avoid known anomalies from this source. The program is written in C++, using OpenGL, GLUT, and GLUI standard libraries, and nVidia GEForce Vertex Shader extensions. The program has been shown to work on several computers running UNIX and Windows operating systems.

  10. Extending particle filters to higher dimensional problems

    Science.gov (United States)

    Weir, B.; Miller, R.; Spitz, Y. H.

    2013-12-01

    Particle filters are attractive solutions to nonlinear and non-Gaussian data assimilation problems since they avoid making parametric assumptions. Nevertheless, in very many dimensions their ensembles collapse onto a single particle unless the number of particles grows exponentially as a function of the dimension. This talk investigates three techniques that, used in conjunction, show the potential of preventing ensemble collapse: optimization, mixture models, and covariance refinement. Optimization is the basis of implicit sampling algorithms. By itself, it significantly reduces the growth of the necessary ensemble size, yet not to a sub-exponential function of dimension. Mixture models, which introduce a semi-parametric assumption, allow the technique to adjust the initial position of the particles. For linear and Gaussian problems, the combination of optimization and mixture models reduces the necessary ensemble size to a sub-exponential function of dimension. Covariance refinement adjusts local approximations of the second moment of the particle distributions to account for its global variation. This is especially effective for problems that are strongly nonlinear. In numerical experiments, covariance refinement used alongside optimization and mixture models shows the potential to extend the prevention of collapse to a general class of nonlinear and non-Gaussian problems.

  11. Economics and extended longevity: a case study.

    Science.gov (United States)

    Gori, G B; Richter, B J; Yu, W K

    1984-07-01

    Preventive and therapeutic advances have brought life expectancy in the United States to well over 70 years and have shifted mortality causes from acute to chronic diseases, the determinants of which are genetics, lifestyle, the environment, and aging itself. Plausible approaches to chronic disease prevention are likely to increase longevity further, with some foreseeable effects on demographic and economic projections. Primarily, longevity advances would swell forecasts of population size, and would thus have to be met by production advances in order to maintain or improve living standards. This study, a restricted example, considers the probable demographic and economic consequences of a limited prevention program in the context of the Ford Motor Company, based on actual experience and certain expectations up to the year 2000. According to the results, prevention would reduce outlays for life insurance, disability, and health care, but would also generate the higher costs of extending pension plans. Undoubtedly, prevention will continue to be highly ranked in society's pursuit of happiness, and society must prepare to meet its effects with appropriate social and economic policies.

  12. Pramipexole extended release: in Parkinson's disease.

    Science.gov (United States)

    Chwieduk, Claudine M; Curran, Monique P

    2010-04-01

    Pramipexole extended release (ER) is a non-ergolinic dopamine receptor agonist available for use as a once-daily oral treatment for the signs and symptoms of early and advanced idiopathic Parkinson's disease. Once-daily pramipexole ER and three times-daily pramipexole immediate release (IR) have similar exposure over 24 hours. The ER formulation is associated with fewer fluctuations in plasma pramipexole concentrations over this period. Pramipexole ER improved the symptoms of Parkinson's disease in three well designed trials in adults with early or advanced disease, as measured by changes from baseline in the sum of the Unified Parkinson's Disease Rating Scale (UPDRS) parts II and III subtotal scores. In a 9-week study, the majority of patients with early Parkinson's disease who were receiving stable pramipexole IR treatment were successfully switched to pramipexole ER. Relative to placebo at week 18, pramipexole ER 0.375-4.5 mg (of the salt) once daily significantly decreased the sum of the UPDRS parts II and III subtotal scores from baseline in two trials in patients with early or advanced Parkinson's disease, and also reduced the percentage of off-time during waking hours in patients with advanced disease. The efficacy of pramipexole ER was maintained after 33 weeks of treatment in the trials in patients with early or advanced Parkinson's disease. Pramipexole ER was generally well tolerated in patients with Parkinson's disease, with the rate of adverse events being generally similar to that with pramipexole IR.

  13. Pramipexole extended release in Parkinson's disease.

    Science.gov (United States)

    Hametner, Eva-Maria; Seppi, Klaus; Poewe, Werner

    2011-09-01

    Pramipexole extended release (ER) is a new once-daily formulation of pramipexole, a nonergot dopamine agonist, which is available in five dosage strengths: 0.26 (0.375) mg, 0.52 (0.75) mg, 1.05 (1.5) mg, 2.1 (3) mg and 3.15 (4.5) mg (all doses are expressed in terms of pramipexole base and the corresponding dose strengths of pramipexole salt are given in brackets). Pramipexole ER is currently approved as monotherapy in early Parkinson's disease (PD), as well as an adjunct therapy to levodopa in advanced PD. Compared with the immediate release (IR) formulation, the ER formulation offers several advantages, including the potential for improved compliance owing to its simple once-daily dosing regimen and steadier plasma levels over 24 h. Double-blind, randomized, placebo and active comparator controlled trials in early, as well as advanced PD, established the superiority of both pramipexole ER and IR over placebo. The overnight switch from pramipexole IR three times a day to ER once-daily in early PD has been shown to be successful in more than 80% of patients. Pramipexole ER is well tolerated, with a similar adverse event profile to pramipexole IR. The aim of this article is to provide a short review of the most relevant pharmacological and clinical data on pramipexole ER.

  14. Extended Life Testing of Duplex Ball Bearings

    Science.gov (United States)

    Mobley, Jeffrey; Robertson, Michael; Hodges, Charles

    2016-01-01

    Sierra Nevada Corporation’s Space Systems performed bearing life testing for the Scan Mirror Motor/Encoder Assembly (SMMA), part of the Scan Mirror Assembly on-board the Aerosol Polarimetry Sensor (APS) on the NASA Glory Spacecraft. The baseline bearing life test duration extended beyond the launch date for the Glory Spacecraft; a risk that the program was willing to undertake with the understanding that if any anomalies or failures occurred before the required life was achieved, then the mission objectives or operating profile could be modified on orbit to take those results into account. Even though the Glory Spacecraft failed to reach orbit during its launch in March of 2011, the bearing life testing was continued through a mutual understanding of value between Sierra Nevada Corporation and our customer; with a revised goal of testing to failure rather than completing a required number of life cycles. Life testing thus far has not only exceeded the original mission required life, but has also exceeded the published test data for Cumulative Degradation Factor (CDF) from NASA/CR-2009-215681. Many lessons were learned along the way regarding long life testing. The bearing life test has been temporarily suspended due to test support equipment issues.

  15. MSTAR extended operating conditions: a tutorial

    Science.gov (United States)

    Keydel, Eric R.; Lee, Shung W.; Moore, John T.

    1996-06-01

    One key advantage of the model-based approach for automatic target recognition (ATR) is the wide range of targets and acquisition scenarios that can be accommodated without algorithm re-training. This accrues from the use of predictive models which can be adjusted to hypothesized scenarios on-line. Approaches which rely on measured signature exemplars as the source of reference data for signature matching are constrained to those scenarios represented in the reference data base. The moving and stationary target recognition (MSTAR) program will advance the state-of-the-art in model-based ATR by developing, evaluating, and testing algorithm performance against a set of extended operating conditions (EOCs) designed to reflect real-world battlefield scenarios. In addition to full 360 deg target aspect coverage over a range of depression angles, the EOCs include variations in squint angle, target articulation and configurations, obscuration due to occlusion and/or layover, and intra-class target variability. These conditions can have a profound impact on the nature of the target signature, necessitating the development of explicit prediction and reasoning algorithms to provide robust target recognition. This paper provides a tutorial description of the impact of the MSTAR EOCs on SAR target signatures. A brief background discussion of the SAR imaging process is presented first. This is followed by a description of the impact of each EOC category on the target signature along with synthetic imagery examples to illustrate this impact.

  16. 14 CFR 23.1511 - Flap extended speed.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Flap extended speed. 23.1511 Section 23.1511 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT... Information § 23.1511 Flap extended speed. (a) The flap extended speed V FE must be established so that it...

  17. Extending Students' Mathematical Thinking during Whole-Group Discussions

    Science.gov (United States)

    Cengiz, Nesrin; Kline, Kate; Grant, Theresa J.

    2011-01-01

    Studies show that extending students' mathematical thinking during whole-group discussions is a challenging undertaking. To better understand what extending student thinking looks like and how teachers' mathematical knowledge for teaching (MKT) supports teachers in their efforts to extend student thinking, the teaching of six experienced…

  18. Extending Students' Mathematical Thinking during Whole-Group Discussions

    Science.gov (United States)

    Cengiz, Nesrin; Kline, Kate; Grant, Theresa J.

    2011-01-01

    Studies show that extending students' mathematical thinking during whole-group discussions is a challenging undertaking. To better understand what extending student thinking looks like and how teachers' mathematical knowledge for teaching (MKT) supports teachers in their efforts to extend student thinking, the teaching of six experienced…

  19. Describing Service-Oriented Architecture by Extended Darwin

    Institute of Scientific and Technical Information of China (English)

    ZHANG Tao; SHEN Mei-e; YING Shi; YE Peng; LIANG Zao-qing

    2005-01-01

    Describing Service-Oriented Architecture (SOA) is critical in the development of Web-based system. In this paper, an approach for describing SOA by extended Darwin is proposed. The requirements for describing SOA, which are different from that of ordinary architecture, are highlighted firstly, and then a solution for extending Darwin is presented. Using the extended Darwin, service components and connectors can be described explicit by the extended construct, as well as precise operational semantics of SOA by the π-calculus. Finally an example of supply-chain management system is given for manifesting the effect of the extended Darwin.

  20. Extending the Schizosaccharomyces pombe molecular genetic toolbox.

    Directory of Open Access Journals (Sweden)

    Dorota Fennessy

    Full Text Available Targeted alteration of the genome lies at the heart of the exploitation of S. pombe as a model system. The rate of analysis is often determined by the efficiency with which a target locus can be manipulated. For most loci this is not a problem, however for some loci, such as fin1+, rates of gene targeting below 5% can limit the scope and scale of manipulations that are feasible within a reasonable time frame. We now describe a simple modification of transformation procedure for directing integration of genomic sequences that leads to a 5-fold increase in the transformation efficiency when antibiotic based dominant selection markers are used. We also show that removal of the pku70+ and pku80+ genes, which encode DNA end binding proteins required for the non-homologous end joining DNA repair pathway, increases the efficiency of gene targeting at fin1+ to around 75-80% (a 16-fold increase. We describe how a natMX6/rpl42+ cassette can be used for positive and negative selection for integration at a targeted locus. To facilitate the evaluation of the impact of a series of mutations on the function of a gene of interest we have generated three vector series that rely upon different selectable markers to direct the expression of tagged/untagged molecules from distinct genomic integration sites. pINTL and pINTK vectors use ura4+ selection to direct disruptive integration of leu1+ and lys1+ respectively, while pINTH vectors exploit nourseothricin resistance to detect the targeted disruption of a hygromycin B resistance conferring hphMX6 cassette that has been integrated on chromosome III. Finally, we have generated a series of multi-copy expression vectors that use resistance to nourseothricin or kanamycin/G418 to select for propagation in prototrophic hosts. Collectively these protocol modifications and vectors extend the versatility of this key model system.

  1. Optimization Testbed Cometboards Extended into Stochastic Domain

    Science.gov (United States)

    Patnaik, Surya N.; Pai, Shantaram S.; Coroneos, Rula M.; Patnaik, Surya N.

    2010-01-01

    COMparative Evaluation Testbed of Optimization and Analysis Routines for the Design of Structures (CometBoards) is a multidisciplinary design optimization software. It was originally developed for deterministic calculation. It has now been extended into the stochastic domain for structural design problems. For deterministic problems, CometBoards is introduced through its subproblem solution strategy as well as the approximation concept in optimization. In the stochastic domain, a design is formulated as a function of the risk or reliability. Optimum solution including the weight of a structure, is also obtained as a function of reliability. Weight versus reliability traced out an inverted-S-shaped graph. The center of the graph corresponded to 50 percent probability of success, or one failure in two samples. A heavy design with weight approaching infinity could be produced for a near-zero rate of failure that corresponded to unity for reliability. Weight can be reduced to a small value for the most failure-prone design with a compromised reliability approaching zero. The stochastic design optimization (SDO) capability for an industrial problem was obtained by combining three codes: MSC/Nastran code was the deterministic analysis tool, fast probabilistic integrator, or the FPI module of the NESSUS software, was the probabilistic calculator, and CometBoards became the optimizer. The SDO capability requires a finite element structural model, a material model, a load model, and a design model. The stochastic optimization concept is illustrated considering an academic example and a real-life airframe component made of metallic and composite materials.

  2. EXTENDED SPEECH EMOTION RECOGNITION AND PREDICTION

    Directory of Open Access Journals (Sweden)

    Theodoros Anagnostopoulos

    2014-11-01

    Full Text Available Humans are considered to reason and act rationally and that is believed to be their fundamental difference from the rest of the living entities. Furthermore, modern approaches in the science of psychology underline that humans as a thinking creatures are also sentimental and emotional organisms. There are fifteen universal extended emotions plus neutral emotion: hot anger, cold anger, panic, fear, anxiety, despair, sadness, elation, happiness, interest, boredom, shame, pride, disgust, contempt and neutral position. The scope of the current research is to understand the emotional state of a human being by capturing the speech utterances that one uses during a common conversation. It is proved that having enough acoustic evidence available the emotional state of a person can be classified by a set of majority voting classifiers. The proposed set of classifiers is based on three main classifiers: kNN, C4.5 and SVM RBF Kernel. This set achieves better performance than each basic classifier taken separately. It is compared with two other sets of classifiers: one-against-all (OAA multiclass SVM with Hybrid kernels and the set of classifiers which consists of the following two basic classifiers: C5.0 and Neural Network. The proposed variant achieves better performance than the other two sets of classifiers. The paper deals with emotion classification by a set of majority voting classifiers that combines three certain types of basic classifiers with low computational complexity. The basic classifiers stem from different theoretical background in order to avoid bias and redundancy which gives the proposed set of classifiers the ability to generalize in the emotion domain space.

  3. Extending and implementing the Persistent ID pillars

    Science.gov (United States)

    Car, Nicholas; Golodoniuc, Pavel; Klump, Jens

    2017-04-01

    The recent double decade anniversary of scholarly persistent identifier use has triggered journal special editions such as "20 Years of Persistent Identifiers". For such a publication, it is apt to consider the longevity of some persistent identifier (PID) mechanisms (Digital Object Identifiers) and the partial disappearance of others (Life Sciences IDs). We have previously postulated a set of "PID Pillars" [1] which are design principles aimed at ensuring PIDs can survive technology and social change and thus persist for the long term that we have drawn from our observations of PIDs at work over many years. The principles: describe how to ensure identifiers' system and organisation independence; codify the delivery of essential PID system functions; mandate a separation of PID functions from data delivery mechanisms; and require generation of policies detailing how change is handled. In this presentation, first we extend on our previous work of introducing the pillars by refining their descriptions, giving specific suggestions for each and presenting some work that addresses them. Second, we propose a baseline data model for persistent identifiers that, if used, would assist the separation of PID metadata and PID system functioning. This would allow PID system function specifics to change over time (e.g. resolver services or even resolution protocols) and yet preserve the PIDs themselves. Third, we detail our existing PID system — the PID Service [2] — that partially implements the pillars and describe both its successes and shortcomings. Finally, we describe our planned next-generation system that will aim to use the baseline data model and fully implement the pillars.

  4. Center for Extended Magnetohydrodynamic Modeling Cooperative Agreement

    Energy Technology Data Exchange (ETDEWEB)

    Carl R. Sovinec

    2008-02-15

    The Center for Extended Magnetohydrodynamic Modeling (CEMM) is developing computer simulation models for predicting the behavior of magnetically confined plasmas. Over the first phase of support from the Department of Energy’s Scientific Discovery through Advanced Computing (SciDAC) initiative, the focus has been on macroscopic dynamics that alter the confinement properties of magnetic field configurations. The ultimate objective is to provide computational capabilities to predict plasma behavior—not unlike computational weather prediction—to optimize performance and to increase the reliability of magnetic confinement for fusion energy. Numerical modeling aids theoretical research by solving complicated mathematical models of plasma behavior including strong nonlinear effects and the influences of geometrical shaping of actual experiments. The numerical modeling itself remains an area of active research, due to challenges associated with simulating multiple temporal and spatial scales. The research summarized in this report spans computational and physical topics associated with state of the art simulation of magnetized plasmas. The tasks performed for this grant are categorized according to whether they are primarily computational, algorithmic, or application-oriented in nature. All involve the development and use of the Non-Ideal Magnetohydrodynamics with Rotation, Open Discussion (NIMROD) code, which is described at http://nimrodteam.org. With respect to computation, we have tested and refined methods for solving the large algebraic systems of equations that result from our numerical approximations of the physical model. Collaboration with the Terascale Optimal PDE Solvers (TOPS) SciDAC center led us to the SuperLU_DIST software library [http://crd.lbl.gov/~xiaoye/SuperLU/] for solving large sparse matrices using direct methods on parallel computers. Switching to this solver library boosted NIMROD’s performance by a factor of five in typical large

  5. Electric refuse collection vehicle with a range extender; Elektrisches Abfallsammelfahrzeug mit Range Extender

    Energy Technology Data Exchange (ETDEWEB)

    Fuchs, Andreas

    2012-10-15

    At the Frankfurt Motor Show IAA 2012, MAN will be presenting the Metropolis, a heavy-duty truck for use in urban areas that produces no emissions and is ultra-quiet. Using mains electricity generated from renewable sources, it can operate without producing any CO{sub 2}. The truck's modular lithium-ion battery is located under the ab. A quiet and efficient diesel engine from the Volkswagen Group generates power as needed and functions as a range extender for the truck. At the end of 2012, the MAN Metropolis will start a two-year field test as a refuse collection vehicle. (orig.)

  6. 14 CFR 25.1511 - Flap extended speed.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Flap extended speed. 25.1511 Section 25... Limitations § 25.1511 Flap extended speed. The established flap extended speed V FE must be established so that it does not exceed the design flap speed V F chosen under §§ 25.335(e) and 25.345, for...

  7. Classical Analog of Extended Phase Space SUSY and Its Breaking

    OpenAIRE

    Gagik Ter-Kazarian

    2013-01-01

    We derive the classical analog of the extended phase space quantum mechanics of the particle with odd degrees of freedom which gives rise to (N=2)-realization of supersymmetry (SUSY) algebra. By means of an iterative procedure, we find the approximate groundstate solutions to the extended Schr\\"{o}dinger-like equation and use these solutions further to calculate the parameters which measure the breaking of extended SUSY such as the groundstate energy. Consequently, we calculate a more practic...

  8. The role of extended family in diverse teens’ sexual health

    OpenAIRE

    Grossman, Jennifer M; Tracy, Allison; Richer,Amanda; Erkut, Sumru

    2014-01-01

    Despite increasing extended family involvement in childrearing, particularly in minority families, few studies investigate their role in talking with teens about sex or how this relates to teens’ sexual behavior. This mixed methods study assesses extended family sexuality communication through a survey of 1492 diverse middle school students and interviews with 32 students. Logistic regression shows that participants who report having had sex are more likely to report talking with extended fam...

  9. Numerical simulation and experimental verification of extended source interferometer

    Science.gov (United States)

    Hou, Yinlong; Li, Lin; Wang, Shanshan; Wang, Xiao; Zang, Haijun; Zhu, Qiudong

    2013-12-01

    Extended source interferometer, compared with the classical point source interferometer, can suppress coherent noise of environment and system, decrease dust scattering effects and reduce high-frequency error of reference surface. Numerical simulation and experimental verification of extended source interferometer are discussed in this paper. In order to provide guidance for the experiment, the modeling of the extended source interferometer is realized by using optical design software Zemax. Matlab codes are programmed to rectify the field parameters of the optical system automatically and get a series of interferometric data conveniently. The communication technique of DDE (Dynamic Data Exchange) was used to connect Zemax and Matlab. Then the visibility of interference fringes can be calculated through adding the collected interferometric data. Combined with the simulation, the experimental platform of the extended source interferometer was established, which consists of an extended source, interference cavity and image collection system. The decrease of high-frequency error of reference surface and coherent noise of the environment is verified. The relation between the spatial coherence and the size, shape, intensity distribution of the extended source is also verified through the analysis of the visibility of interference fringes. The simulation result is in line with the result given by real extended source interferometer. Simulation result shows that the model can simulate the actual optical interference of the extended source interferometer quite well. Therefore, the simulation platform can be used to guide the experiment of interferometer which is based on various extended sources.

  10. An extended phase-space SUSY quantum mechanics

    Energy Technology Data Exchange (ETDEWEB)

    Ter-Kazarian, G [Byurakan Astrophysical Observatory, Byurakan 378433, Aragatsotn District (Armenia)], E-mail: gago_50@yahoo.com

    2009-02-06

    In the present paper, we will concern ourselves with the extended phase-space quantum mechanics of particles which have both bosonic and fermionic degrees of freedom, i.e., the quantum field theory in (0 + 1) dimensions in q-(position) and p-(momentum) spaces, exhibiting supersymmetry. We present (N = 2) realization of extended supersymmetry algebra and discuss the vacuum energy and topology of super-potentials. Shape invariance of exactly solvable extended SUSY potentials allows us to obtain analytic expressions for the entire energy spectrum of an extended Hamiltonian with, for example, Scarf potential without ever referring to an underlying differential equation.

  11. Liposomal extended-release bupivacaine for postsurgical analgesia

    National Research Council Canada - National Science Library

    Lambrechts, Mark; O'Brien, Michael J; Savoie, Felix H; You, Zongbing

    2013-01-01

    ..., inguinal hernia repair, total knee arthroplasty, and augmentation mammoplasty. However, like other bupivacaine formulations, the liposomal extended-release bupivacaine does have some side effects...

  12. Collaborative Proposal to Extend ONR YIP Research with BRC Efforts

    Science.gov (United States)

    2013-09-30

    DISTRIBUTION STATEMENT: Approved for public release; distribution is unlimited. Collaborative Proposal to Extend ONR YIP research with BRC Efforts...SEP 2013 2. REPORT TYPE 3. DATES COVERED 00-00-2013 to 00-00-2013 4. TITLE AND SUBTITLE Collaborative Proposal to Extend ONR YIP research with

  13. An Extended Ockham Algebra with Endomorphism Kernel Property

    Institute of Scientific and Technical Information of China (English)

    Jie FANG

    2007-01-01

    An algebraic structure (∮) is said to have the endomorphism kernel property if every congruence on (∮) , other than the universal congruence, is the kernel of an endomorphism on (∮) .Inthis paper, we consider the EKP (that is, endomorphism kernel property) for an extended Ockham algebra (∮) . In particular, we describe the structure of the finite symmetric extended de Morgan algebras having EKP.

  14. LR-parsing of Extended Context-free Grammars

    DEFF Research Database (Denmark)

    Madsen, Ole Lehrmann; Kristensen, Bent Bruun

    1976-01-01

    To improve the readability of a grammar it is common to use extended context free grammars (ECFGs) which are context free grammars (CFGs) extended with the repetition operator (*), the alternation operator (¦) and parentheses to express the right hand sides of the productions. The topic treated h...

  15. Extending Gaussian hypergeometric series to the $p$-adic setting

    CERN Document Server

    McCarthy, Dermot

    2012-01-01

    We define a function which extends Gaussian hypergeometric series to the $p$-adic setting. This new function allows results involving Gaussian hypergeometric series to be extended to a wider class of primes. We demonstrate this by providing various congruences between the function and truncated classical hypergeometric series. These congruences provide a framework for proving the supercongruence conjectures of Rodriguez-Villegas.

  16. Polyurethanes elastomers with amide chain extenders of uniform length

    NARCIS (Netherlands)

    van der Schuur, J.M.; Noordover, B.A.J.; Noordover, Bart; Gaymans, R.J.

    2006-01-01

    Toluene diisocyanate based polyurethanes with amide extenders were synthesized poly(propylene oxide) with a number average molecular weight of 2000 and endcapped with toluene diisocyanate was used as the polyether segment. The chain extenders were based on poly(hexamethylene terephthalamide):

  17. Subsurface offset behaviour in velocity analysis with extended reflectivity images

    NARCIS (Netherlands)

    Mulder, W.A.

    2012-01-01

    Migration velocity analysis with the wave equation can be accomplished by focusing of extended migration images, obtained by introducing a subsurface offset or shift. A reflector in the wrong velocity model will show up as a curve in the extended image. In the correct model, it should collapse to a

  18. Subsurface offset behaviour in velocity analysis with extended reflectivity images

    NARCIS (Netherlands)

    Mulder, W.A.

    2013-01-01

    Migration velocity analysis with the constant-density acoustic wave equation can be accomplished by the focusing of extended migration images, obtained by introducing a subsurface shift in the imaging condition. A reflector in a wrong velocity model will show up as a curve in the extended image. In

  19. Polyurethane elastomers with amide chain extenders of uniform length

    NARCIS (Netherlands)

    Schuur, van der Martijn; Noordover, Bart; Gaymans, Reinoud J.

    2006-01-01

    Toluene diisocyanate based polyurethanes with amide extenders were synthesized poly(propylene oxide) with a number average molecular weight of 2000 and endcapped with toluene diisocyanate was used as the polyether segment. The chain extenders were based on poly(hexamethylene terephthalamide): hexame

  20. [Extended endoscopic endonasal transsphenoidal approaches in skull base surgery].

    Science.gov (United States)

    Kalinin, P L; Fomichev, D V; Kutin, M A; Kadashev, B A; Faĭzullaev, R B

    2008-01-01

    The article deals with endoscopic endonasal transsphenoidal surgery, which has gained great interest among the modem trends of neurosurgery. Application of extended endoscopic endonasal transsphenoidal approaches significantly advances capabilities of transsphenoidal surgery. Pituitary adenomas and some other sellar tumors which traditionally require transcranial procedure now can be removed via endonasal route. The article describes several types of extended endoscopic endonasal transsphenoidal approaches.

  1. The accuracy of extended histopathology to detect immunotoxic chemicals

    NARCIS (Netherlands)

    Germolec, D.R.; Kashon, M.; Nyska, A.; Kuper, C.F.; Portier, C.; Kommineni, C.; Johnson, K.A.; Luster, M.I.

    2004-01-01

    The accuracy of extended histopathology to detect immunotoxic chemicals in female B6C3F1 mice was evaluated under the auspices of the National Toxicology Program (NTP). A workgroup was formed consisting of four pathologists who conducted extended histopathological evaluation of lymphoid tissues

  2. Application of Noether's theorem to extended particles

    Energy Technology Data Exchange (ETDEWEB)

    Smida, A; Hachemane, M; Hamici, A H [Faculte de Physique, USTHB B.P.32 El-Alia Bab-Ezzouar 16111 Alger (Algeria)], E-mail: hachemane@wissal.dz

    2008-08-15

    We consider an approach similar to field theory for the application of Noether theorem to extended paticles. We obtain Euler-Lagrange equations for the extended particles as well as an equation binding the internal and external currents. The concrete case of spin 1/2 is considered in detail.

  3. Propagation of extended objects across singularity of time dependent orbifold

    CERN Document Server

    Malkiewicz, Przemyslaw

    2010-01-01

    In this paper we argue that the compactified Milne space is a promising model of the cosmological singularity. It is shown that extended objects like strings propagate in a well-defined manner across the singularity of the embedding space. Then a proposal for quantization of extended objects in the case of a membrane is given.

  4. The role of infant appetite in extended formula feeding

    NARCIS (Netherlands)

    Syrad, H.; Jaarsveld, C.H.M. van; Wardle, J.; Llewellyn, C.H.

    2015-01-01

    OBJECTIVE: Parental decision-making around extended formula feeding (12 months+) has not been explored previously. This study tested the hypotheses that extended formula milk use (i) is associated with poorer appetite and (ii) supplements lower food intake. METHODS: Appetite was assessed with the Ch

  5. Review of extended producer responsibility: A case study approach.

    Science.gov (United States)

    Gupt, Yamini; Sahay, Samraj

    2015-07-01

    Principles of extended producer responsibility have been the core of most of the recent policies and legislation dealing with the end-of-life management of recyclable goods. This article makes an exploratory review of 27 cases of extended producer responsibility from developed and developing economies with and without informal recycling, to ascertain the most important aspect of extended producer responsibility. A comparative analysis of the cases with respect to role of stakeholders in the upstream and downstream stages of the extended producer responsibility has been carried out. Further, the study uses exploratory factor analysis to determine the important aspects of the extended producer responsibility in practice using 13 variables identified from the review. Findings of the comparative analysis reveal that financial responsibility of the producers and separate collecting and recycling agencies contributed significantly to the success of the extended producer responsibility-based environmental policies. Regulatory provisions, take-back responsibility and financial flow come out to be the three most important aspects of the extended producer responsibility. Presence of informal sector had a negative impact on the regulatory provisions. The outcomes of this study could serve as a guideline for designing of effective extended producer responsibility-based policies. © The Author(s) 2015.

  6. Extended Fuzzy Logic Programs with Fuzzy Answer Set Semantics

    Science.gov (United States)

    Saad, Emad

    This paper extends fuzzy logic programs [12, 24] to allow the explicit representation of classical negation as well as non-monotonic negation, by introducing the notion of extended fuzzy logic programs. We present the fuzzy answer set semantics for the extended fuzzy logic programs, which is based on the classical answer set semantics of classical extended logic programs [7]. We show that the proposed semantics is a natural extension to the classical answer set semantics of classical extended logic programs [7]. Furthermore, we define fixpoint semantics for extended fuzzy logic programs with and without non-monotonic negation, and study their relationship to the fuzzy answer set semantics. In addition, we show that the fuzzy answer set semantics is reduced to the stable fuzzy model semantics for normal fuzzy logic programs introduced in [42]. The importance of that is computational methods developed for normal fuzzy logic programs can be applied to the extended fuzzy logic programs. Moreover, we show that extended fuzzy logic programs can be intuitively used for representing and reasoning about actions in fuzzy environment.

  7. The extended Kerr-Schild approach to general relativity

    CERN Document Server

    Wang, Xun

    2016-01-01

    We study in some detail the "extended Kerr-Schild" formulation of general relativity, which decomposes the gauge-independent degrees of freedom of a generic metric into two arbitrary functions and the choice of a flat background tetrad. We recast Einstein's equations and spacetime curvatures in the extended Kerr-Schild form and discuss their properties, illustrated with simple examples.

  8. Extended Logistic Chaotic Sequence and Its Performance Analysis

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xuefeng; FAN Jiulun

    2007-01-01

    In order to improve performance and security of image encryption algorithm effectively based on chaotic sequences, an extended chaotic sequence generating method is presented based on logistic chaotic system using Bernstein form Bezier curve generating algorithm. In order to test the pseudorandom performance of the extended chaotic sequence, we also analyze random performance, autocorrelation performance, and balance performance of the extended chaotic sequence. Simulation results show that the extended chaotic sequence generated using our method is pseudorandom and its correlation performance and balance performance are good. As an application, we apply the extended chaotic sequence in image encryption algorithm, the simulation results show that the performance of the encrypted image using our method is better than that using logistic chaotic sequence.

  9. The scattering amplitude for rationally extended shape invariant Eckart potentials

    Energy Technology Data Exchange (ETDEWEB)

    Yadav, Rajesh Kumar, E-mail: rajeshastrophysics@gmail.com [Department of Physics, Banaras Hindu University, Varanasi-221005 (India); Khare, Avinash, E-mail: khare@iiserpune.ac.in [Raja Ramanna Fellow, Indian Institute of Science Education and Research (IISER), Pune-411021 (India); Mandal, Bhabani Prasad, E-mail: bhabani.mandal@gmail.com [Department of Physics, Banaras Hindu University, Varanasi-221005 (India)

    2015-01-23

    Highlights: • Bound states of rationally extended Eckart potentials have been discussed. • These potentials exhibit extended shape invariant properties. • The potentials which are isospectral to the conventional Eckart potential are considered. • The scattering amplitude of these potentials has been obtained. • For a check, m=0 provide the scattering amplitude for the conventional potential. - Abstract: We consider the rationally extended exactly solvable Eckart potentials which exhibit extended shape invariance property. These potentials are isospectral to the conventional Eckart potential. The scattering amplitude for these rationally extended potentials is calculated analytically for the generalized mth (m=1,2,3,...) case by considering the asymptotic behavior of the scattering state wave functions which are written in terms of some new polynomials related to the Jacobi polynomials. As expected, in the m=0 limit, this scattering amplitude goes over to the scattering amplitude for the conventional Eckart potential.

  10. Mutant INS-gene induced diabetes of youth: proinsulin cysteine residues impose dominant-negative inhibition on wild-type proinsulin transport.

    Directory of Open Access Journals (Sweden)

    Ming Liu

    Full Text Available Recently, a syndrome of Mutant INS-gene-induced Diabetes of Youth (MIDY, derived from one of 26 distinct mutations has been identified as a cause of insulin-deficient diabetes, resulting from expression of a misfolded mutant proinsulin protein in the endoplasmic reticulum (ER of insulin-producing pancreatic beta cells. Genetic deletion of one, two, or even three alleles encoding insulin in mice does not necessarily lead to diabetes. Yet MIDY patients are INS-gene heterozygotes; inheritance of even one MIDY allele, causes diabetes. Although a favored explanation for the onset of diabetes is that insurmountable ER stress and ER stress response from the mutant proinsulin causes a net loss of beta cells, in this report we present three surprising and interlinked discoveries. First, in the presence of MIDY mutants, an increased fraction of wild-type proinsulin becomes recruited into nonnative disulfide-linked protein complexes. Second, regardless of whether MIDY mutations result in the loss, or creation, of an extra unpaired cysteine within proinsulin, Cys residues in the mutant protein are nevertheless essential in causing intracellular entrapment of co-expressed wild-type proinsulin, blocking insulin production. Third, while each of the MIDY mutants induces ER stress and ER stress response; ER stress and ER stress response alone appear insufficient to account for blockade of wild-type proinsulin. While there is general agreement that ultimately, as diabetes progresses, a significant loss of beta cell mass occurs, the early events described herein precede cell death and loss of beta cell mass. We conclude that the molecular pathogenesis of MIDY is initiated by perturbation of the disulfide-coupled folding pathway of wild-type proinsulin.

  11. Function of the integrin alpha 6 beta 1 in metastatic breast carcinoma cells assessed by expression of a dominant-negative receptor

    DEFF Research Database (Denmark)

    Shaw, L M; Chao, C; Wewer, U M;

    1996-01-01

    The involvement of the alpha 6 beta a integrin, a laminin receptor, in breast carcinoma progression needs to be addressed rigorously. We report that a human breast carcinoma cell line, MDA-MB-435, known to be highly invasive and metastatic, expresses three potential integrin laminin receptors...... function that involved expression of a cytoplasmic domain deletion mutant of the beta 4 integrin subunit by cDNA transfection. Stable transfectants of MDA-MB-435 cells that expressed this mutant beta 4 subunit were inhibited dramatically in their ability to adhere and migrate on laminin matrices......, and their capacity to invade Matrigel was reduced significantly. These findings support the hypothesis that alpha 6 beta 1 is important for breast cancer progression. Moreover, this approach is a powerful method that should be useful in assessing the role of alpha 6 beta 1 in other cells....

  12. Dominant negative phenotype of Bacillus thuringiensis Cry1Ab, Cry11Aa and Cry4Ba mutants suggest hetero-oligomer formation among different Cry toxins.

    NARCIS (Netherlands)

    Carmona, D.; Rodriguez-Almazan, C.; Munoz-Garay, C.; Portugal, L.; Perez, C.; Maagd, de R.A.; Bakker, P.; Soberon, M.; Bravo, A.

    2011-01-01

    Background - Bacillus thuringiensis Cry toxins are used worldwide in the control of different insect pests important in agriculture or in human health. The Cry proteins are pore-forming toxins that affect the midgut cell of target insects. It was shown that non-toxic Cry1Ab helix a-4 mutants had a d

  13. Expression of a Catalytically Inactive Mutant Form of Glutathione Peroxidase 4 (Gpx4) Confers a Dominant-negative Effect in Male Fertility.

    Science.gov (United States)

    Ingold, Irina; Aichler, Michaela; Yefremova, Elena; Roveri, Antonella; Buday, Katalin; Doll, Sebastian; Tasdemir, Adrianne; Hoffard, Nils; Wurst, Wolfgang; Walch, Axel; Ursini, Fulvio; Friedmann Angeli, José Pedro; Conrad, Marcus

    2015-06-05

    The selenoenzyme Gpx4 is essential for early embryogenesis and cell viability for its unique function to prevent phospholipid oxidation. Recently, the cytosolic form of Gpx4 was identified as an upstream regulator of a novel form of non-apoptotic cell death, called ferroptosis, whereas the mitochondrial isoform of Gpx4 was previously shown to be crucial for male fertility. Here, we generated and analyzed mice with a targeted mutation of the active site selenocysteine of Gpx4 (Gpx4_U46S). Mice homozygous for Gpx4_U46S died at the same embryonic stage (E7.5) as Gpx4(-/-) embryos as expected. Surprisingly, male mice heterozygous for Gpx4_U46S presented subfertility. Subfertility was manifested in a reduced number of litters from heterozygous breeding and an impairment of spermatozoa to fertilize oocytes in vitro. Morphologically, sperm isolated from heterozygous Gpx4_U46S mice revealed many structural abnormalities particularly in the spermatozoa midpiece due to improper oxidation and polymerization of sperm capsular proteins and malformation of the mitochondrial capsule surrounding and stabilizing sperm mitochondria. These findings are reminiscent of sperm isolated from selenium-deprived rodents or from mice specifically lacking mitochondrial Gpx4. Due to a strongly facilitated incorporation of Ser in the polypeptide chain as compared with selenocysteine at the UGA codon, expression of the catalytically inactive Gpx4_U46S was found to be strongly increased. Because the stability of the mitochondrial capsule of mature spermatozoa depends on the moonlighting function of Gpx4 both as an enzyme oxidizing capsular protein thiols and as a structural protein, tightly controlled expression of functional Gpx4 emerges as a key for full male fertility.

  14. MALT1 cleaves the E3 ubiquitin ligase HOIL-1 in activated T cells, generating a dominant negative inhibitor of LUBAC-induced NF-κB signaling.

    Science.gov (United States)

    Elton, Lynn; Carpentier, Isabelle; Staal, Jens; Driege, Yasmine; Haegman, Mira; Beyaert, Rudi

    2016-02-01

    Human paracaspase 1 (PCASP1), better known as mucosa associated lymphoid tissue lymphoma translocation 1 (MALT1), plays a key role in immunity and inflammation by regulating gene expression in lymphocytes and other immune cell types. Deregulated MALT1 activity has been implicated in autoimmunity, immunodeficiency and certain types of lymphoma. As a scaffold MALT1 assembles downstream signaling proteins for nuclear factor-κB (NF-κB) activation, while its proteolytic activity further enhances NF-κB activation by cleaving NF-κB inhibitory proteins. MALT1 also processes and inactivates a number of mRNA destabilizing proteins, which further fine-tunes gene expression. MALT1 protease inhibitors are currently developed for therapeutic targeting. Here we show that T cell activation, as well as overexpression of the oncogenic fusion protein API2-MALT1, induces the MALT1-mediated cleavage of haem-oxidized IRP2 ubiquitin ligase 1 (HOIL-1). In addition, to acting as a K48-polyubiquitin specific E3 ubiquitin ligase for different substrates, HOIL-1 co-operates in a catalytic-independent manner with the E3 ubiquitin ligase HOIL-1L interacting protein (HOIP) as part of the linear ubiquitin chain assembly complex (LUBAC). Intriguingly, cleavage of HOIL-1 does not directly abolish its ability to support HOIP-induced NF-κB signaling, which is still mediated by the N-terminal cleavage fragment, but generates a C-terminal fragment with LUBAC inhibitory properties. We propose that MALT1-mediated HOIL-1 cleavage provides a gain-of-function mechanism that is involved in the negative feedback regulation of NF-κB signaling.

  15. The expression of dominant negative TCF7L2 in pancreatic beta cells during the embryonic stage causes impaired glucose homeostasis

    Directory of Open Access Journals (Sweden)

    Weijuan Shao

    2015-04-01

    Conclusions: Our observations support a cell autonomous role for TCF7L2 in pancreatic β-cells suggested by most, though not all, investigations. βTCFDN is a novel model for further exploring the role of TCF7L2 in β-cell genesis and metabolic homeostasis.

  16. Rice Brittle culm 6 encodes a dominant-negative form of CesA protein that perturbs cellulose synthesis in secondary cell walls.

    Science.gov (United States)

    Kotake, Toshihisa; Aohara, Tsutomu; Hirano, Ko; Sato, Ami; Kaneko, Yasuko; Tsumuraya, Yoichi; Takatsuji, Hiroshi; Kawasaki, Shinji

    2011-03-01

    The brittle culm (bc) mutants of Gramineae plants having brittle skeletal structures are valuable materials for studying secondary cell walls. In contrast to other recessive bc mutants, rice Bc6 is a semi-dominant bc mutant with easily breakable plant bodies. In this study, the Bc6 gene was cloned by positional cloning. Bc6 encodes a cellulose synthase catalytic subunit, OsCesA9, and has a missense mutation in its highly conserved region. In culms of the Bc6 mutant, the proportion of cellulose was reduced by 38%, while that of hemicellulose was increased by 34%. Introduction of the semi-dominant Bc6 mutant gene into wild-type rice significantly reduced the percentage of cellulose, causing brittle phenotypes. Transmission electron microscopy analysis revealed that Bc6 mutation reduced the cell wall thickness of sclerenchymal cells in culms. In rice expressing a reporter construct, BC6 promoter activity was detected in the culms, nodes, and flowers, and was localized primarily in xylem tissues. This expression pattern was highly similar to that of BC1, which encodes a COBRA-like protein involved in cellulose synthesis in secondary cell walls in rice. These results indicate that BC6 is a secondary cell wall-specific CesA that plays an important role in proper deposition of cellulose in the secondary cell walls.

  17. Mutant INS-Gene Induced Diabetes of Youth: Proinsulin Cysteine Residues Impose Dominant-Negative Inhibition on Wild-Type Proinsulin Transport

    OpenAIRE

    Ming Liu; Leena Haataja; Jordan Wright; Wickramasinghe, Nalinda P.; Qing-Xin Hua; Phillips, Nelson F.; Fabrizio Barbetti; Weiss, Michael A; Peter Arvan

    2010-01-01

    Recently, a syndrome of M utant I NS-gene-induced D iabetes of Y outh (MIDY , derived from one of 26 distinct mutations) has been identified as a cause of insulin-deficient diabetes, resulting from expression of a misfolded mutant proinsulin protein in the endoplasmic reticulum (ER) of insulin-producing pancreatic beta cells. Genetic deletion of one, two, or even three alleles encoding insulin in mice does not necessarily lead to diabetes. Yet MIDY patients are INS-gene heterozygotes; inherit...

  18. SREBP-1 dimerization specificity maps to both the helix-loop-helix and leucine zipper domains: use of a dominant negative

    DEFF Research Database (Denmark)

    Rishi, Vikas; Gal, Jozsef; Krylov, Dmitry

    2004-01-01

    -SREBP-1 consists of the dimerization domain of B-SREBP-1 and a polyglutamic acid sequence that replaces the basic region. A-SREBP-1 heterodimerizes with either B-SREBP-1 or B-SREBP-2, and both heterodimers are more stable than B-SREBP-1 bound to DNA. Circular dichroism thermal denaturation studies show...

  19. Heteromeric p97/p97R155C complexes induce dominant negative changes in wild-type and autophagy 9-deficient Dictyostelium strains.

    Directory of Open Access Journals (Sweden)

    Khalid Arhzaouy

    Full Text Available Heterozygous mutations in the human VCP (p97 gene cause autosomal-dominant IBMPFD (inclusion body myopathy with early onset Paget's disease of bone and frontotemporal dementia, ALS14 (amyotrophic lateral sclerosis with or without frontotemporal dementia and HSP (hereditary spastic paraplegia. Most prevalent is the R155C point mutation. We studied the function of p97 in the social amoeba Dictyostelium discoideum and have generated strains that ectopically express wild-type (p97 or mutant p97 (p97(R155C fused to RFP in AX2 wild-type and autophagy 9 knock-out (ATG9(KO cells. Native gel electrophoresis showed that both p97 and p97(R155C assemble into hexamers. Co-immunoprecipitation studies revealed that endogenous p97 and p97(R155C-RFP form heteromers. The mutant strains displayed changes in cell growth, phototaxis, development, proteasomal activity, ubiquitinylated proteins, and ATG8(LC3 indicating mis-regulation of multiple essential cellular processes. Additionally, immunofluorescence analysis revealed an increase of protein aggregates in ATG9(KO/p97(R155C-RFP and ATG9(KO cells. They were positive for ubiquitin in both strains, however, solely immunoreactive for p97 in the ATG9(KO mutant. A major finding is that the expression of p97(R155C-RFP in the ATG9(KO strain partially or fully rescued the pleiotropic phenotype. We also observed dose-dependent effects of p97 on several cellular processes. Based on findings in the single versus the double mutants we propose a novel mode of p97 interaction with the core autophagy protein ATG9 which is based on mutual inhibition.

  20. Cut and Paste: Efficient Homology-Directed Repair of a Dominant Negative KRT14 Mutation via CRISPR/Cas9 Nickases.

    Science.gov (United States)

    Kocher, Thomas; Peking, Patricia; Klausegger, Alfred; Murauer, Eva Maria; Hofbauer, Josefina Piñón; Wally, Verena; Lettner, Thomas; Hainzl, Stefan; Ablinger, Michael; Bauer, Johann Wolfgang; Reichelt, Julia; Koller, Ulrich

    2017-08-24

    With the ability to induce rapid and efficient repair of disease-causing mutations, CRISPR/Cas9 technology is ideally suited for gene therapy approaches for recessively and dominantly inherited monogenic disorders. In this study, we have corrected a causal hotspot mutation in exon 6 of the keratin 14 gene (KRT14) that results in generalized severe epidermolysis bullosa simplex (EBS-gen sev), using a double-nicking strategy targeting intron 7, followed by homology-directed repair (HDR). Co-delivery into EBS keratinocytes of a Cas9 D10A nickase (Cas9n), a predicted single guide RNA pair specific for intron 7, and a minicircle donor vector harboring the homology donor template resulted in a recombination efficiency of >30% and correction of the mutant KRT14 allele. Phenotypic correction of EBS-gen sev keratinocytes was demonstrated by immunofluorescence analysis, revealing the absence of disease-associated K14 aggregates within the cytoplasm. We achieved a promising safety profile for the CRISPR/Cas9 double-nicking approach, with no detectable off-target activity for a set of predicted off-target genes as confirmed by next generation sequencing. In conclusion, we demonstrate a highly efficient and specific gene-editing approach for KRT14, offering a causal treatment option for EBS. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

  1. Population aging and the extended family in Taiwan

    Directory of Open Access Journals (Sweden)

    2004-05-01

    Full Text Available Population aging produces changes in the availability of kin with uncertain implications for extended living arrangements. We propose a highly stylized model that can be used to analyze and project age-specific proportions of adults living in extended and nuclear households. The model is applied to Taiwan using annual data from 1978-1998. We estimate cohort and age effects showing that more recently born cohorts of seniors are less likely to live in extended households, but that as seniors age the proportion living in extended households increases. The effect of individual aging has diminished over time, however. The proportion of non-senior adults living in extended households has increased steadily because changes in the age structure have increased the availability of older kin. The model is used to project living arrangements and we conclude that the proportion living in extended households will begin to decline gradually for both seniors and non-seniors. The extended family is becoming less important in Taiwan, but it is not on the way out.

  2. Interdigitated Extended Gate Field Effect Transistor Without Reference Electrode

    Science.gov (United States)

    Ali, Ghusoon M.

    2017-02-01

    An interdigitated extended gate field effect transistor (IEGFET) has been proposed as a modified pH sensor structure of an extended gate field effect transistor (EGFET). The reference electrode and the extended gate in the conventional device have been replaced by a single interdigitated extended gate. A metal-semiconductor-metal interdigitated extended gate containing two multi-finger Ni electrodes based on zinc oxide (ZnO) thin film as a pH-sensitive membrane. ZnO thin film was grown on a p-type Si (100) substrate by the sol-gel technique. The fabricated extended gate is connected to a commercial metal-oxide-semiconductor field-effect transistor device in CD4007UB. The experimental data show that this structure has real time and linear pH voltage and current sensitivities in a concentration range between pH 4 and 11. The voltage and current sensitivities are found to be about 22.4 mV/pH and 45 μA/pH, respectively. Reference electrode elimination makes the IEGFET device simple to fabricate, easy to carry out the measurements, needing a small volume of solution to test and suitable for disposable biosensor applications. Furthermore, this uncomplicated structure could be extended to fabricate multiple ions microsensors and lab-on-chip devices.

  3. Extended radio emission and the nature of blazars

    Energy Technology Data Exchange (ETDEWEB)

    Antonucci, R.R.J.; Ulvestad, J.S.

    1985-07-01

    The VLA has been used at 20 cm to map all 23 of the 54 confirmed blazars listed in the Angel and Stockman review paper that had not been mapped before at high resolution. (Blazars include BL Lac objects and optically violently variable quasars.) In addition, data on most of the previously mapped blazars have been reprocessed in order to achieve higher dynamic range. Extended emission has been detected associated with 49 of the 54 objects. The extended radio emission has been used to test the hypothesis that blazars are normal radio galaxies and radio quasars viewed along the jet axes. We find that blazars have substantial extended power, consistent with this hypothesis. Many have extended powers as high as the luminous Fanaroff-Riley class 2 radio doubles. The projected linear sizes are small, as expected from foreshortening of the extended sources, and many blazars have the expected core-halo morphology. There are also several small doubles, a head-tail source, and some one-sided sources, and these could be in cases where the line of sight is slightly off the jet axis, or projections of asymmetrical radio galaxies and quasars. The ratio of core to extended radio emission has been studied as a possible indicator of viewing aspect or beaming intensity. It is found to correlate with optical polarization, optical and radio core variability, and one-sided radio morphology. We can go beyond these consistency checks and work toward a proof of the hypothesis under discussion. The flux from the extended emission alone is sufficient in some blazars to qualify them for inclusion in the 3C and 4C catalogs. Suppose that the radio core emission is anisotropic, but the extended emission is predominantly isotropic. The isotropy of the extended emission implies that these blazars would be in the catalogs even if viewed from the side.

  4. Octonionic Realizations of 1-dimensional Extended Supersymmetries. A Classification

    CERN Document Server

    Carrion, H L; Toppan, F

    2003-01-01

    The classification of the octonionic realizations of the one-dimensional extended supersymmetries is here furnished. These are non-associative realizations which, albeit inequivalent, are put in correspondence with a subclass of the already classified associative representations for 1D extended supersymmetries. Examples of dynamical systems invariant under octonionic realizations of the extended supersymmetries are given. We cite among the others the octonionic spinning particles, the N=8 KdV, etc. Possible applications to supersymmetric systems arising from dimensional reduction of the octonionic superstring and M-theory are mentioned.

  5. Octonionic realizations of 1-dimensional extended supersymmetries. A classification

    Energy Technology Data Exchange (ETDEWEB)

    Carrion, H.L.; Rojas, M.; Toppan, F. [Centro Brasileiro de Pesquisas Fisicas (CBPF), Rio de Janeiro, RJ (Brazil). Coordenacao de Teoria de Campos e Particulas]. E-mail: hleny@cbpf.br; mrojas@cbpf.br; toppan@cbpf.br

    2002-12-01

    The classification of the octonionic realizations of the one-dimensional extended supersymmetries is here furnished. These are non-associative realizations which, albeit inequivalent, are put in correspondence with a subclass of the already classified associative representations for 1D extended supersymmetries. Examples of dynamical systems invariant under octonionic realizations of the extended supersymmetries are given. We cite among the others the octonionic spinning particles, the N = 8 KdV, etc. Possible applications to supersymmetric systems arising from dimensional reduction of the octonionic superstring and M-theory are mentioned. (author)

  6. Extended Riemannian Geometry I: Local Double Field Theory

    CERN Document Server

    Deser, Andreas

    2016-01-01

    We present an extended version of Riemannian geometry suitable for the description of current formulations of double field theory (DFT). This framework is based on graded manifolds and it yields extended notions of symmetries, dynamical data and constraints. In special cases, we recover general relativity with and without 1-, 2- and 3-form gauge potentials as well as DFT. We believe that our extended Riemannian geometry helps to clarify the role of various constructions in DFT. For example, it leads to a covariant form of the strong section condition. Furthermore, it should provide a useful step towards global and coordinate invariant descriptions of T- and U-duality invariant field theories.

  7. An Examination of Extended a-Rescaling Model

    Institute of Scientific and Technical Information of China (English)

    YAN Zhan-Yuan; DUAN Chun-Gui; HE Zhen-Min

    2001-01-01

    The extended x-rescaling model can explain the quark's nuclear effect very well. Weather it can also explain the gluon's nuclear effect should be investigated further. Associated J/ψ and γ production with large PT is a very clean channel to probe the gluon distribution in proton or nucleus. In this paper, using the extended x-rescaling model, the PT distribution of the nuclear effect factors of p + Fe → J/Ψ + γ+ X process is calculated and discussed. Comparing our theoretical results with the future experimental data, the extended x-rescaling model can be examined.``

  8. Symposium on quantum electronics. Extended abstracts of contributed papers

    Energy Technology Data Exchange (ETDEWEB)

    1981-02-01

    Extended abstracts are provided for papers presented in these subject areas: lasers; quantum optics; nonlinear optics; laser photochemistry; laser spectroscopy; laser scattering; laser produced plasma; and laser applications. (GHT)

  9. A note on extending decision algorithms by stable predicates

    Directory of Open Access Journals (Sweden)

    Alfredo Ferro

    1988-11-01

    Full Text Available A general mechanism to extend decision algorithms to deal with additional predicates is described. The only conditions imposed on the predicates is stability with respect to some transitive relations.

  10. Structured plan and its execution for extended goals

    Institute of Scientific and Technical Information of China (English)

    HUANG Wei; PENG Hong

    2009-01-01

    In many real world planning domains, some observation information is optional and useless to the execution of a plan, and the information acquisition may require some kind of cost. So it is significant to propose a framework for planning with extended goals under partial observability, which can adapt to the idea of observation reduction. In this paper, a method was proposed to solve the problem. The definition of structured plans was given, which can encode sequential, conditional and iterative behaviors, and are expressive enough for dealing with incomplete observation information and with extended goals. In order to express extended goals over sub-plans and observation information, the interpretation of basic propositions in K-CTL and EAGLE (two important languages for expressing temporal requirements in nondeterministic domains) was extended.

  11. An engineering approach to extending lifespan in C. elegans.

    Directory of Open Access Journals (Sweden)

    Dror Sagi

    Full Text Available We have taken an engineering approach to extending the lifespan of Caenorhabditis elegans. Aging stands out as a complex trait, because events that occur in old animals are not under strong natural selection. As a result, lifespan can be lengthened rationally using bioengineering to modulate gene expression or to add exogenous components. Here, we engineered longer lifespan by expressing genes from zebrafish encoding molecular functions not normally present in worms. Additionally, we extended lifespan by increasing the activity of four endogenous worm aging pathways. Next, we used a modular approach to extend lifespan by combining components. Finally, we used cell- and worm-based assays to analyze changes in cell physiology and as a rapid means to evaluate whether multi-component transgenic lines were likely to have extended longevity. Using engineering to add novel functions and to tune endogenous functions provides a new framework for lifespan extension that goes beyond the constraints of the worm genome.

  12. Extended Reconstructed Sea Surface Temperature (ERSST), Version 4

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Extended Reconstructed Sea Surface Temperature (ERSST) dataset is a global monthly sea surface temperature analysis on a 2x2 degree grid derived from the...

  13. Optimal control problem for the extended Fisher–Kolmogorov equation

    Indian Academy of Sciences (India)

    Ning Duan

    2016-02-01

    In this paper, the optimal control problem for the extended Fisher–Kolmogorov equation is studied. The optimal control under boundary condition is given, the existence of optimal solution to the equation is proved and the optimality system is established.

  14. Quantum potential and symmetries in extended phase space

    CERN Document Server

    Nasiri, S

    2005-01-01

    Here, we study the concept of the quantum potential using an extended phase space technique. It seems that, for a given potential, there exist an extended canonical transformation that removes the expression for quantum potential in dynamical equation. The situation, mathematically, is similar to the appearance of centrifugal potential in going from Cartesian to spherical coordinates that changes the physical potential to an effective one. As Examples, the cases of harmonic oscillator, particle in a box and hydrogen atom are worked out, where the quantum potential disappears from the Wigner equation as a possible representation of quantum mechanics in the phase space. This representation that keeps the Hamilton-Jacobi equation form invariant could be obtained by a particular extended canonical transformation on Sobouti-Nasiri equation in extended phase space.

  15. Construction of extended exponential general linear methods 524 ...

    African Journals Online (AJOL)

    Construction of extended exponential general linear methods 524 for solving semi-linear problems. ... Journal Home > Vol 13, No 2 (2014) > ... This paper introduces a new approach for constructing higher order of EEGLM which have become ...

  16. The entity-to-algorithm allocation problem: Extending the analysis

    CSIR Research Space (South Africa)

    Grobler, J

    2014-12-01

    Full Text Available This paper extends the investigation into the algorithm selection problem in hyper-heuristics, otherwise referred to as the entity-to-algorithm allocation problem, introduced by Grobler et al.. Two newly developed population-based portfolio...

  17. Extended Supersymmetric BMS$_3$ algebras and Their Free Field Realisations

    CERN Document Server

    Banerjee, Nabamita; Lodato, Ivano; Mukhi, Sunil; Neogi, Turmoli

    2016-01-01

    We study $N=(2,4,8)$ supersymmetric extensions of the three dimensional BMS algebra (BMS$_3$) with most generic possible central extensions. We find that $N$-extended supersymmetric BMS$_3$ algebras can be derived by a suitable contraction of two copies of the extended superconformal algebras. Extended algebras from all the consistent contractions are obtained by scaling left-moving and right-moving supersymmetry generators symmetrically, while Virasoro and R-symmetry generators are scaled asymmetrically. On the way, we find that the BMS/GCA correspondence does not in general hold for supersymmetric systems. Using the $\\beta$-$\\gamma$ and the ${\\mathfrak b}$-${\\mathfrak c}$ systems, we construct free field realisations of all the extended super-BMS$_3$ algebras.

  18. Extending the unambiguous velocity range using multiple carrier frequencies

    DEFF Research Database (Denmark)

    Zhang, Z.; Jakobsson, A.; Nikolov, Svetoslav;

    2005-01-01

    Typically, velocity estimators based on the estimation of the Doppler shift will suffer from a limited unambiguous velocity range. Proposed are two novel multiple carrier based velocity estimators extending the velocity range above the Nyquist velocity limit. Numerical simulations indicate...

  19. Asymptotic Theory for Extended Asymmetric Multivariate GARCH Processes

    NARCIS (Netherlands)

    M. Asai (Manabu); M.J. McAleer (Michael)

    2016-01-01

    textabstractThe paper considers various extended asymmetric multivariate conditional volatility models, and derives appropriate regularity conditions and associated asymptotic theory. This enables checking of internal consistency and allows valid statistical inferences to be drawn based on empirical

  20. Classical analog of extended phase space SUSY and its breaking

    CERN Document Server

    Ter-Kazarian, Gagik

    2013-01-01

    We derive the classical analog of the extended phase space quantum mechanics of the particle with odd degrees of freedom which gives rise to (N=2)-realization of supersymmetry (SUSY) algebra. By means of an iterative procedure, we find the approximate groundstate solutions to the extended Schr\\"{o}dinger-like equation and use these solutions further to calculate the parameters which measure the breaking of extended SUSY such as the groundstate energy. Consequently, we calculate a more practical measure for the SUSY breaking which is the expectation value of an auxiliary field. We analyze non-perturbative mechanism for extended phase space SUSY breaking in the instanton picture and show that this has resulted from tunneling between the classical vacua of the theory. Particular attention is given to the algebraic properties of shape invariance and spectrum generating algebra.

  1. extended pedicle rectus abdominis myocutaneous flap for groin

    African Journals Online (AJOL)

    Damary

    2005-03-10

    Mar 10, 2005 ... a flabby and redundant abdominal tissue is used as a huge extended ... functional muscle in head and neck. This flap is ... the aneurysm and adjacent muscle planes. The distal ... Histology of the excised portion of aneurysmal.

  2. Extended Homogeneous Balance Method and Lax Pairs, Backlund Transformation

    Institute of Scientific and Technical Information of China (English)

    BAI ChengLin

    2002-01-01

    Using the extended homogeneous balance method, which is very concise and primary, Lax pairs and Backlund transformation for most nonlinear evolution equations, such as the compound KdV-Burgers equation and nonlinear diffusion equation are obtained.

  3. In-Situ Extended Lateral Range Surface Metrology Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose to develop an extended lateral range capability for a dynamic optical profiling system to enable non-contact, surface roughness measurement of large and...

  4. comparison of three different extenders and storage temperature on ...

    African Journals Online (AJOL)

    HelenM

    extenders protect sperm against seminal plasma which can be toxic, while providing temperature protection ... sperm cell waste, ions for membrane and cell balance, and antibiotics to prevent growth of ... of sperm to perform their function.

  5. Extended quintessence, inflation, and stable de Sitter spaces

    CERN Document Server

    Faraoni, V; Faraoni, Valerio; Jensen, Michael N.

    2006-01-01

    A new gauge-invariant criterion for stability against inhomogeneous perturbations of de Sitter space is applied to scenarios of dark energy and inflation in scalar-tensor gravity. The results extend previous studies.

  6. 34 CFR 300.106 - Extended school year services.

    Science.gov (United States)

    2010-07-01

    ... year services must be provided only if a child's IEP Team determines, on an individual basis, in..., or duration of those services. (b) Definition. As used in this section, the term extended school...

  7. Sky pixelization for the analysis of extended emission

    Science.gov (United States)

    Verkhodanov, O. V.; Doroshkevich, A. G.

    2013-08-01

    Spherical pixelization schemes are reviewed that allow analyzing extended emission and, in particular, the cosmic microwave background. Problems with implementing different schemes are considered. The nonhierarchical Gauss-Legendre sky pixelization (GLESP) approach is discussed in detail.

  8. Optical technologies in extended-reach access networks

    DEFF Research Database (Denmark)

    Wong, Elaine; Amaya Fernández, Ferney Orlando; Tafur Monroy, Idelfonso

    2009-01-01

    The merging of access and metro networks has been proposed as a solution to lower the unit cost of customer bandwidth. This paper reviews some of the recent advances and challenges in extended-reach optical access networks....

  9. Analogy to Derive an Extended Pythagorean Theorem to ''N'' Dimensions

    Directory of Open Access Journals (Sweden)

    Acosta-Robledo J.U.

    2012-01-01

    Full Text Available This article demonstrates that it is possible to extend the Pythagorean Theorem to ''N'' dimensions. This demonstration is mainly done based on linear algebra, especially in the vector product of ''N'' dimensions.

  10. Creating a Generic Extended Enterprise Management Model using GERAM

    DEFF Research Database (Denmark)

    Larsen, Lars Bjørn; Kaas-Pedersen, Carsten; Vesterager, Johan

    1998-01-01

    The two main themes of the Globeman21 (Global Manufacturing in the 21st century) project are product life cycle management and extended enterprise management. This article focus on the later of these subjects and an illustration of the concept is given together with a discussion of the concept...... management model. By working with GERAM in relation to extended enterprise management it has been found that it provides a useful background for organising knowledge, experience and the activities within the project...... of virtual enterprises. Through the introduction of GERAM (Generalised Enterprise Reference Architecture and Methodology) an initial version of a basic framework for extended enterprise management is introduced. This basic framework is the first step towards the creation of a generic extended enterprise...

  11. Extended Quark Potential Model From Random Phase Approximation

    Institute of Scientific and Technical Information of China (English)

    DENGWei-Zhen; CHENXiao-Lin; 等

    2002-01-01

    The quark potential model is extended to include the sea quark excitation using the random phase approximation.The effective quark interaction preserves the important QCD properties-chiral symmetry and confinement simultaneously.A primary qualitative analysis shows that the π meson as a well-known typical Goldstone boson and the other mesons made up of valence qq quark pair such as the ρ meson can also be described in this extended quark potential model.

  12. Extended Quark Potential Model from Random Phase Approximation

    Institute of Scientific and Technical Information of China (English)

    DENG Wei-Zhen; CHEN Xiao-Lin; LU Da-Hai; YANG Li-Ming

    2002-01-01

    The quark potential model is extended to include the sea quark excitation using the random phase approx-imation. The effective quark interaction preserves the important QCD properties - chiral symmetry and confinementsimultaneously. A primary qualitative analysis shows that the π meson as a well-known typical Goldstone boson andthe other mesons made up of valence qq quark pair such as the ρ meson can also be described in this extended quarkpotential model.

  13. An extended Coleman-Noll procedure for generalized continuum theories

    Science.gov (United States)

    Hütter, Geralf

    2016-11-01

    Within rational continuum mechanics, the Coleman-Noll procedure is established to derive requirements to constitutive equations. Aiming in particular at generalized continuum theories, the present contribution demonstrates how this procedure can be extended to yield additionally the underlying balance equations of stress-type quantities. This is demonstrated for micromorphic and strain gradient media as well as for the microforce theory. The relation between the extended Coleman-Noll procedure and the method of virtual powers is pointed out.

  14. Chaotic synchronization in coupled spatially extended beam-plasma systems

    OpenAIRE

    Filatov, Roman A.; Hramov, Alexander E.; ALEXEY A. KORONOVSKII

    2006-01-01

    The appearance of the chaotic synchronization regimes has been discovered for the coupled spatially extended beam-plasma Pierce systems. The coupling was introduced only on the right bound of each subsystem. It has been shown that with coupling increase the spatially extended beam-plasma systems show the transition from asynchronous behavior to the phase synchronization and then to the complete synchronization regime. For the consideration of the chaotic synchronization we used the concept of...

  15. Extended Goldstone-boson-exchange constituent quark model

    CERN Document Server

    Wagenbrunn, R F; Plessas, W; Varga, K

    2000-01-01

    We discuss an updated version of the Goldstone-boson-exchange chiral quark model extended to include in addition to pseudoscalar meson exchanges also vector and scalar meson exchanges. The latter ingredients are viewed as effective parametrizations of multiple Goldstone-boson exchanges in baryons. The extended model allows for an accurate description of all light and strange baryon spectra and at the same time produces the right properties for deducing baryon-baryon interactions.

  16. Extended phenotype: nematodes turn ants into bird-dispersed fruits

    DEFF Research Database (Denmark)

    Hughes, D P; Kronauer, D J C; Boomsma, J J

    2008-01-01

    A recent study has discovered a novel extended phenotype of a nematode which alters its ant host to resemble ripe fruit. The infected ants are in turn eaten by frugivorous birds that disperse the nematode's eggs.......A recent study has discovered a novel extended phenotype of a nematode which alters its ant host to resemble ripe fruit. The infected ants are in turn eaten by frugivorous birds that disperse the nematode's eggs....

  17. Extended treatment for cigarette smoking cessation: a randomized control trial.

    Science.gov (United States)

    Laude, Jennifer R; Bailey, Steffani R; Crew, Erin; Varady, Ann; Lembke, Anna; McFall, Danielle; Jeon, Anna; Killen, Diana; Killen, Joel D; David, Sean P

    2017-08-01

    To test the potential benefit of extending cognitive-behavioral therapy (CBT) relative to not extending CBT on long-term abstinence from smoking. Two-group parallel randomized controlled trial. Patients were randomized to receive non-extended CBT (n = 111) or extended CBT (n = 112) following a 26-week open-label treatment. Community clinic in the United States. A total of 219 smokers (mean age: 43 years; mean cigarettes/day: 18). All participants received 10 weeks of combined CBT + bupropion sustained release (bupropion SR) + nicotine patch and were continued on CBT and either no medications if abstinent, continued bupropion + nicotine replacement therapy (NRT) if increased craving or depression scores, or varenicline if still smoking at 10 weeks. Half the participants were randomized at 26 weeks to extended CBT (E-CBT) to week 48 and half to non-extended CBT (no additional CBT sessions). The primary outcome was expired CO-confirmed, 7-day point-prevalence (PP) at 52- and 104-week follow-up. Analyses were based on intention-to-treat. PP abstinence rates at the 52-week follow-up were comparable across non-extended CBT (40%) and E-CBT (39%) groups [odds ratio (OR) = 0.99; 95% confidence interval (CI) = 0.55, 1.78]. A similar pattern was observed across non-extended CBT (39%) and E-CBT (33%) groups at the 104-week follow-up (OR = 0.79; 95% CI= 0.44, 1.40). Prolonging cognitive-behavioral therapy from 26 to 48 weeks does not appear to improve long-term abstinence from smoking. © 2017 Society for the Study of Addiction.

  18. Solvability of Extended General Strongly Mixed Variational Inequalities

    Directory of Open Access Journals (Sweden)

    Balwant Singh Thakur

    2013-10-01

    Full Text Available In this paper, a new class of extended general strongly mixed variational inequalities is introduced and studied in Hilbert spaces. An existence theorem of solution is established and using resolvent operator technique, a new iterative algorithm for solving the extended general strongly mixed variational inequality is suggested. A convergence result for the iterative sequence generated by the new algorithm is also established.

  19. Extending Particle Swarm Optimisers with Self-Organized Criticality

    DEFF Research Database (Denmark)

    Løvbjerg, Morten; Krink, Thiemo

    2002-01-01

    Particle swarm optimisers (PSOs) show potential in function optimisation, but still have room for improvement. Self-organized criticality (SOC) can help control the PSO and add diversity. Extending the PSO with SOC seems promising reaching faster convergence and better solutions.......Particle swarm optimisers (PSOs) show potential in function optimisation, but still have room for improvement. Self-organized criticality (SOC) can help control the PSO and add diversity. Extending the PSO with SOC seems promising reaching faster convergence and better solutions....

  20. International Symposium on Halide Glasses (2nd) (Extended Abstracts).

    Science.gov (United States)

    1983-08-05

    method in which Pyrex 7740 is the standard material. These results will be compared with our earlier results on a fluorozirconate glass ( ZBLAN glass ...AliS 215 INTERNATIONAL SYMPOSIUM ON HALIDE GLASSES 12ND) 1/1 (EXTENDED ABSTRACTS) (U) RENSSELAER POLY’TECHNIC INST TROY NY DEPT OF MATERIALS ENGINEE...Classification) Second International Symposium on Halide Glasses (Extended Abstracts) (U) 12. PERSONAL AUTHOR(S) Cornelius T. Moynihan Chairman 13a