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Sample records for direct pulp capping

  1. Outcomes of direct pulp capping: interrogating an insurance database.

    Science.gov (United States)

    Raedel, M; Hartmann, A; Bohm, S; Konstantinidis, I; Priess, H W; Walter, M H

    2016-11-01

    To evaluate the effectiveness of direct pulp capping under general practice conditions. It was hypothesized that direct pulp capping is an effective procedure in the majority of cases and prevents the need for root canal treatment or extraction. Claims data were collected from the digital database of a major German national health insurance company. Only patients who had been insurance members for the entire 3 year period 2010 to 2012 were eligible. Kaplan-Meier survival analyses were conducted for all teeth with direct pulp capping. Success was defined as not undergoing root canal treatment. Survival was defined as not undergoing extraction. Differences between survival functions were tested with the log rank test. A total of 148 312 teeth were included. The overall success rate was 71.6% at 3 years. The overall survival rate was 95.9% at 3 years. The success rates for single-rooted teeth (71.8%) and multirooted teeth (71.5%) were similar although significantly different (P 85 years.). After direct pulp capping, more than two-thirds of the affected teeth did not undergo root canal treatment within 3 years. Although this study has the typical limits of a claims data analysis, it can be concluded that direct pulp capping is an effective intervention to avoid root canal treatment and extraction in a general practice setting. © 2015 International Endodontic Journal. Published by John Wiley & Sons Ltd.

  2. DIRECT PULP CAPPING IN TREATMENT OF REVERSIBLE PULPITIS IN PRIMARY TEETH- CLINICAL PROTOCOL

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    Nina Milcheva

    2016-10-01

    Full Text Available The pulp of primary teeth is identical morphologically and physiologically to that of permanent teeth and it is capable to answer to pathological stimuli by producing tertiary dentin. When the inflammation of the pulp is in its reversible stage vital methods of treatment are indicated in order to stimulate the healing processes in it and protect its vitality. In Bulgaria the most popular method of treatment of inflammation diseases of the pulp in primary dentition is the mortal amputation. The biological way of treatment is not very common even in cases where there are indications for it. Purpose: The aim of this paper is to present the approbated by us protocol for application of direct pulp capping for treatment of reversible pulpitis in primary teeth. Material and methods: On the base of world experience and our contemporary meta- analysis of the researches published in the last 15 years concerning the problems of diagnostics. We determined clinical and radiographic diagnostic criteria for reversible pulpitis in primary teeth and indications for application of direct pulp capping as a method of treatment. We give clinical steps for application of the method and summarized the clinical and radiographic criteria for success after treatment. Results/conclusion: We gather all the information for applying direct pulp cappingfor treatment of reversible pulpitis in primary dentition. We offer the method of direct pulp capping as a clinical protocol “step by step” and illustrated by scheme which can be useful for students and dentists in their everyday practice.

  3. Preventive endodontics by direct pulp capping with restorative dentin substitute-biodentine: A series of fifteen cases

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    Kavita Dube

    2018-01-01

    Full Text Available Introduction: Treatment of mechanical exposure of the pulp during caries excavation presents a clinical challenge. In this case series of 15 patients, with a follow-up period of over a year, the outcome of direct pulp capping with Biodentine (septodont after mechanical pulp exposure was assessed. Aim of Study: The aim of this study is to evaluate the outcome of direct pulp capping with Biodentine in deeply carious teeth when pulp was mechanically exposed during caries excavation and cavity preparation. Vital pulps exposed during caries excavation in molar teeth were treated with 3% sodium hypochlorite for 2 min. If adequate hemostasis was achieved, the pulp tissue was capped with Biodentine, which covered the entire pulpal floor. This was followed by the placement of a layer of resin-modified glass ionomer cement and a final layer of composite resin (Filtek Z350-3M to complete the restoration. The patients were recalled periodically and evaluated for any evidence of pulpal/periapical pathology. Results: In the follow-up period that ranged from 12 to 24 months, all teeth were asymptomatic. Conclusion: Biodentine appears to be a suitable material for direct pulp capping under clinical conditions. However, long-term follow-up studies and controlled trials involving a large sample size are warranted.

  4. A Histopathological Study of Direct Pulp Capping with Adhesive Resins

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    J. Salhenejad

    2004-12-01

    Full Text Available Statement of Problem: Recently, it has been proposed that different adhesive materials can be used for direct pulp capping. Previous studies have demonstrated that multi steps dentin adhesives could form reparative dentin similar to calcium hydroxide (CH.Purpose: The aim of this study was to evaluate the histological pulp response of ninety mechanically exposed cat pulps to two adhesive resins (Scotch Bond MP and Single Bond 3M were compared with a calcium hydroxide cement (Dycal, Dentsply.Materials and Methods : Class V facial cavities with similar pulpal exposures were prepared in canines. In the experimental groups phosphoric acid was used to etch the enamel and dentin and pulp exposure, and after it dentin adhesives was applied. The exposure point of the control group was capped with Dycal then the remainder of the cavities was etched and a dentin adhesive (single bond was applied. All of the cavities were restored with a composite resin (Z 100 in usual manner. The animals were scarified after 7, 30 and 60 days (n=30, and the pulp evaluated histologically, statistical analysis was carried out with Kruskal- Wallis test (a=0.05.Results: The data showed that most of the cases had mild inflammation of pulp tissue.There was no significant difference in inflammatory reaction of pulp by Dycal and two adhesive systems, severe inflammatory reaction of pulp was observed only in most of the 30- day Single Bond group. Soft tissue organization of dentin bridge was less than ScotchBond and Dycal groups, the differentiation of dentin bridge was less than Scotch Bond group after 7 days.Conclusion: Slight inflammatory cell infiltration was the main reaction of exposed pulp when two commercially available adhesive resins were placed directly on the exposed pulp.There was no significant difference in inflammatory reaction of pulp between Dycal and two adhesive systems after 7 days and 60 days. After 7 days most of the specimens showed an amount of predentin

  5. Mineral Trioxide Aggregate and Portland Cement for Direct Pulp Capping in Dog: A Histopathological Evaluation

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    Maryam Bidar

    2014-09-01

    Full Text Available Background and aims. Mineral trioxide aggregate and calcium hydroxide are considered the gold standard pulp-capping materials. Recently, Portland cement has been introduced with properties similar to those of mineral trioxide aggregate. His-topathological effects of direct pulp capping using mineral trioxide aggregate and Portland cements on dog dental pulp tis-sue were evaluated in the present study. Materials and methods. This histopatological study was carried out on 64 dog premolars. First, the pulp was exposedwith a sterile bur. Then, the exposed pulp was capped with white or gray mineral trioxide aggregates and white or gray Port-land cements in each quadrant and sealed with glass-ionomer. The specimens were evaluated under a light microscope after 6 months. Statistical analysis was carried out using Kruskal-Wallis test. Statistical significance was defined at α=5%. Results. There was no acute inflammation in any of the specimens. Chronic inflammation in white and gray mineral triox-ide aggregates and white and gray Portland cements was reported to be 45.5%, 27.3%, 57.1% and 34.1%, respectively. Al-though the differences were not statistically significant, severe inflammation was observed mostly adjacent to white mineral trioxide aggregate. The largest extent of increased vascularization (45% and the least increase in fibrous tissue were ob-served adjacent to white mineral trioxide aggregate, with no significant differences. In addition, the least calcified tissue formed adjacent to white mineral trioxide aggregate, although the difference was not significant. Conclusion. The materials used in this study were equally effective as pulp protection materials following direct pulp cap-ping in dog teeth.

  6. Mineral Trioxide Aggregate (MTA direct pulp capping: 10 years clinical results

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    Lucio Daniele

    2017-06-01

    After 10-years follow-up period, 6 out of 80 teeth required an endodontic treatment, we did not find an increase of pulp calcifications, root resorptions, endodontic lesions. All teeth still alive have responded positively to the cold-temperature test. The three lesions had disappeared at the last X-ray control. All four symptomatic teeth preserved their pulp vitality. The successful treatment rate was 92.5%. Direct pulp capping with MTA can be considered an effective treatment of exposure due to tooth decay and can be considered a valid treatment option for teeth with a diagnosis of not-severe reversible pulpitis.

  7. The increasing of odontoblast-like cell number on direct pulp capping of Rattus norvegicus using chitosan

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    Prananingrum, Widyasri

    2010-01-01

    Background: Pulpal perforation care with direct pulp capping in the case of reversible pulpitis due to mechanical trauma was performed with chitosan which has the ability to facilitate migration, proliferation, and progenitor cell differentiation. Purpose: The purpose of this study was to determine the increasing number of odontoblast-like cells in direct pulp capping dental care of Rattus norvegicus using chitosan for seven and fourteen days. Methods: Samples were molars of male Rattus norve...

  8. The increasing of odontoblast-like cell number on direct pulp capping of Rattus norvegicus using chitosan

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    Widyasri Prananingrum

    2010-12-01

    Full Text Available Background: Pulpal perforation care with direct pulp capping in the case of reversible pulpitis due to mechanical trauma was performed with chitosan which has the ability to facilitate migration, proliferation, and progenitor cell differentiation. Purpose: The purpose of this study was to determine the increasing number of odontoblast-like cells in direct pulp capping dental care of Rattus norvegicus using chitosan for seven and fourteen days. Methods: Samples were molars of male Rattus norvegicus strain wistar, aged between 8–16 weeks, divided into two treatment groups, namely group I given chitosan and group II as a control group given Ca(OH2. Those Rattus norvegicus’ occlusal molar teeth were prepared with class I cavity, and then chitosan and Ca(OH2 were applied as the pulp capping materials. Afterwards, glasss ionomer cement type IX was used as a restoration material. Their teeth and jaw were then cut on the seventh day and the fourteenth day. Next, histopathological examination was carried out to observe the odontoblast like cells. All data were then analyzed by t test. Degree of confidence obtained, finally, was 95%. Results: The results obtained showed that the significant differences of odontoblast like cells on the seventh day observation was 0.001 (p = 0.001, and on the fourteenth day observation was 0.002 (p = 0.002. Conclusion: The number of odontoblast-like cells in direct pulp capping dental care of rattus norvegicus using chitosan is higher than the one using Ca(OH2 for seven and fourteen days.Latar belakang: Perawatan perforasi pulpa pada kasus pulpitis reversible karena trauma mekanis bur dilakukan direct pulp capping dengan cara pemberian bahan secara topikal pada daerah perforasi. Kitosan memiliki kemampuan untuk memfasilitasi migrasi, proliferasi dan diferensiasi sel progenitor pulpa. Tujuan: Tujuan penelitian ini adalah untuk menentukan jumlah peningkatan odontoblas-like cell pada perawatan direct pulp capping gigi

  9. Pulp-Capping with Mineral Trioxide Aggregate

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    Peycheva Kalina

    2015-11-01

    Full Text Available There are two considerations for direct pulp capping - accidental mechanical pulp exposure and exposure caused by caries. Mineral trioxide aggregate (MTA was used as pulp-capping material to preserve the vitality of the pulpal tissues. Follow-up examinations revealed that treatment was successful in preserving pulpal vitality and continued development of the tooth. On the basis of available information, it appears that MTA is the material of choice for some clinical applications. Material and methods: Cases 18 - 8 teeth with grey MTA, 10 teeth with white MTA; diagnose: Pulpitis chronica ulcerosa, Electro pulpal test (EOD - 30-35 μA, pre-clinical X-ray - without changes in the structures, follow ups for 4 years. Successful treatments: without clinical symptoms and changes in the X-rays: 5 teeth with grey MTA, 8 teeth with white MTA for period of 4 years. Unsuccessful treatments: Clinical symptoms and sometimes changes in the X-ray: 3 with grey MTA, 2 with white MTA. MTA is an appropriate material for pulp-capping and follow-up examinations revealed that the treatment was successful in preserving pulpal vitality.

  10. Comparison of Calcium Hydroxide and Bioactive Glass after Direct Pulp Capping in Primary Teeth

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    R. Haghgoo

    2007-12-01

    Full Text Available Objective: Bioactive glass is often used as a filler material for repair of dental bone defects.In different studies osteogenic potential of this material was proved, but its dentinogenesisproperty is in doubt. The purpose of this study was to evaluate the histological pulp responses of Calcium hydroxide and Bioactive glass placed directly on exposed pulp tissues.Materials and Methods: Twenty teeth to be extracted due to orthodontic reasons were selected. These teeth were divided into two groups and treated with direct pulp capping.Calcium hydroxide was used for 10 teeth and Bioactive glass for 10 teeth. After 60 daysthe teeth were extracted and prepared for histological evaluation. Finally the data was analyzed with exact Fisher test.Results: All teeth treated with Calcium hydroxide showed inflammation. Internal resorption was seen in six teeth, abscess in five teeth and dentinal bridge in two teeth. Inflammationwas seen in three Bioactive glass samples and dentinal bridge in seven teeth, but internal resorption and abscess were not seen.Conclusion: Bioactive glass appears to be superior to Calcium hydroxide as a pulp capping agent in primary teeth.

  11. Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice.

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    Song, Minju; Kim, Sol; Kim, Terresa; Park, Sil; Shin, Ki-Hyuk; Kang, Mo; Park, No-Hee; Kim, Reuben

    2017-01-12

    Dental pulp is a vital organ of a tooth fully protected by enamel and dentin. When the pulp is exposed due to cariogenic or iatrogenic injuries, it is often capped with biocompatible materials in order to expedite pulpal wound healing. The ultimate goal is to regenerate reparative dentin, a physical barrier that functions as a "biological seal" and protects the underlying pulp tissue. Although this direct pulp-capping procedure has long been used in dentistry, the underlying molecular mechanism of pulpal wound healing and reparative dentin formation is still poorly understood. To induce reparative dentin, pulp capping has been performed experimentally in large animals, but less so in mice, presumably due to their small sizes and the ensuing technical difficulties. Here, we present a detailed, step-by-step method of performing a pulp-capping procedure in mice, including the preparation of a Class-I-like cavity, the placement of pulp-capping materials, and the restoration procedure using dental composite. Our pulp-capping mouse model will be instrumental in investigating the fundamental molecular mechanisms of pulpal wound healing in the context of reparative dentin in vivo by enabling the use of transgenic or knockout mice that are widely available in the research community.

  12. Bioceramic/Poly (glycolic-poly (lactic acid composite induces mineralized barrier after direct capping of rat tooth pulp tissue

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    Alfonso Gala-Garcia

    2010-03-01

    Full Text Available The aim of this study was to observe the histopathological pulp response following direct pulp capping of mechanically exposed teeth in rats with a composite of beta-tricalcium phosphate-hydroxyapatite bioceramic (BC and poly (glycolic-poly (lactic acid (PLGA material or a calcium hydroxide [Ca(OH2] material, compared to BC alone and a negative control of water. Pulp of the maxillary molars was exposed, followed by capping with the experimental material. The pulpal tissue response was assessed post-operatively at 1, 7, 14 and 30 d, followed by histological analysis. The Ca(OH2 group exhibited severe acute inflammatory cell infiltration at day 14. However after 30 d, a new hard tissue with macro porous obliteration of the pulp chamber and a characteristic necrotic area had appeared. BC and Ca(OH2 capping were associated with moderate inflammation and dentinal bridge similar. Meanwhile, in the BC/PLGA composite group, there was moderate inflammatory infiltrate and formation of a dense and complete dentinal bridge. In conclusion, the BC/PLGA composite material showed a large zone of tertiary dentin, and effectively reorganized the dentin-pulp complex.

  13. Histological evaluation of direct pulp capping of rat pulp with experimentally developed low-viscosity adhesives containing reparative dentin-promoting agents.

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    Suzuki, Masaya; Taira, Yoshihisa; Kato, Chikage; Shinkai, Koichi; Katoh, Yoshiroh

    2016-01-01

    This study examines the wound healing process in exposed rat pulp when capped with experimental adhesive resin systems. Experimental adhesive resin system for direct pulp capping was composed of primer-I (PI), -II (PII), and -III (PIII) and an experimental bonding agent (EBA). PI was Clearfil(®) SE Bond(®)/Primer (CSP) containing 5.0 wt% CaCl2, PII was PI containing 10 wt% nanofiller (Aerosil(®) 380), and PIII was CSP containing 5.0 wt% of compounds of equal moles of synthetic peptides (pA and pB) derived from dentin matrix protein 1. EBA was Clearfil(®) SE Bond(®)/Bond (CSB) containing 10 wt% hydroxyapatite powders. Three experimental groups were designed. PI was assigned to experimental Groups 1 and 3. PII was assigned to experimental Groups 2 and 3. PIII and EBA were assigned to all experimental adhesive groups. Control teeth were capped with calcium hydroxide preparation (Dycal(®)), and CSP and CSB were applied to the cavity. The rats were sacrificed after each observation period (14, 28, 56, and 112 days). The following parameters were evaluated: pulp tissue disorganization, inflammatory cell infiltration, reparative dentin formation (RDF), and bacterial penetration. There were no significant differences among all the groups for all parameters and all observation periods (p>0.05, Kruskal-Wallis test). All groups showed initial RDF at 14 days postoperatively and extensive RDF until 112 days postoperatively. Groups 2 and 3 demonstrated higher quantity of mineralized dentin bridge formation compared with Group 1. Addition of nanofillers to the primer was effective in promoting high-density RDF. Experimentally developed adhesive resin systems induce the exposed pulp to produce almost the same quantity of reparative dentin as calcium hydroxide. However, we need further studies to elucidate whether the same results could be obtained in humans. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. Pulp tissue inflammation and angiogenesis after pulp capping with transforming growth factor β1

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    Sri Kunarti

    2008-06-01

    Full Text Available In Restorative dentistry the opportunity to develop biomemitic approaches has been signalled by the possible use of various biological macromolecules in direct pulp capping reparation. The presence of growth factors in dentin matrix and the putative role indicating odontoblast differentiation during embryogenesis has led to the examination on the effect of endogenous TGF-β1. TGF-β1 is one of the Growth Factors that plays an important role in pulp healing. The application of exogenous TGF-β1 in direct pulp capping treatment should be experimented in fibroblast tissue in-vivo to see the responses of inflammatory cells and development of new blood vessels. The increase in food supplies always occurs in the process of inflammation therefore the development of angiogenesis is required to fulfil the requirement. This in-vivo study done on orthodontic patients indicated for premolar extraction between 10–15 years of age. A class V cavity preparation was created in the buccal aspect 1 mm above gingival margin to pulp exposure. The cavity was slowly irrigated with saline solution and dried using a sterile small cotton pellet. The sterile absorbable collagen membrane was applied and soaked in 5 ml TGF-β1. It was covered by a Teflon pledge to separate from Glass Ionomer Cement restoration. Evaluation was performed on day 7; 14; and 21. All samples were histopathologycally examined and data was statistically analysed using one way ANOVA and Dunnet T3.There were no inflammatory symptoms in clinical examination on both Ca(OH2 and TGF-β1, but they increased the infiltration of inflammatory cells on histopathological examination. There were no significant differences (p > 0.05 between Ca(OH2 and TGF-β1 in inflammation cell and significant differences (p < 0.05 in angiogenesis on day 7 and 14. There were no significant differences (p > 0.05 in inflammation cell with in TGF-β1 groups and significant differences (p < 0.05 with in Ca(OH2 groups on day 7

  15. Direct Pulp Capping with Calcium Hydroxide, Mineral Trioxide Aggregate, and Biodentine in Permanent Young Teeth with Caries: A Randomized Clinical Trial.

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    Brizuela, Claudia; Ormeño, Andrea; Cabrera, Carolina; Cabezas, Roxana; Silva, Carolina Inostroza; Ramírez, Valeria; Mercade, Montse

    2017-11-01

    Direct pulp capping treatment is intended to preserve pulp vitality, to avoid or retard root canal treatment, and, in cases with an open apex, to allow continued root development. Historically, calcium hydroxide (CH) was the gold standard material, but nowadays calcium silicate materials (CSMs) are displacing CH because of their high bioactivity, biocompatibility, sealing ability, and mechanical properties. However, more randomized clinical trials are needed to confirm the appropriateness of CSMs as replacement materials for CH in direct pulp capping procedures. A randomized clinical trial was conducted that included 169 patients (mean age, 11.3 years) from the Maipo district (Chile). The inclusion criterion was patients with 1 carious permanent tooth with pulpal exposure, a candidate for a direct pulp capping procedure. The patients were randomly allocated to one of the experimental groups (CH, Biodentine, or mineral trioxide aggregate [MTA]). Clinical follow-up examinations were performed at 1 week, 3 months, 6 months, and 1 year. The Fisher exact test was performed. At the follow-up examination at 1 week, the patients showed 100% clinical success. At 3 months, there was 1 failure in the CH group. At 6 months, there were 4 new failures (1 in the CH group and 3 in the MTA group). At 1 year, there was another failure in the CH group. There were no statistically significant differences among the experimental groups. CSMs appear to be suitable materials to replace CH. Although no significant differences were found among the materials studied, Biodentine and MTA offered some advantages over CH. Copyright © 2017 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  16. Indirect pulp capping in primary molar using glass ionomer cements

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    Murtia Metalita

    2014-12-01

    Full Text Available Background: Indirect pulp capping in primary teeth, however, is more rarely conducted than permanent teeth, since it thought to have low impact and most suggestion is for taking caries lesion aggressively on primary teeth. Purpose: The study was aimed to evaluate the subjective complaint, clinical symptom, and radiographic appearance of indirect pulp capping treatment using glass ionomers cements in primary molar. Methods: Sixteen children in range of age 6 to 8 years old, who visited Clinic of Pediatric Dentistry Universitas Airlangga Dental Hospital, Surabaya Indonesia, were the subject of study. They had one occlusal dental caries on one side of maxillary or mandibular primary molar with the diagnose of pulpitis reversible. The experimental group, had indirect pulp capping treatment with glass ionomer cements (GC Fuji VII®, while the control group, had indirect pulp capping treatment with calcium hydroxide (Metapaste. Each group was filled with GC Fuji IX® as permanent restoration. After one week, one month, and three months later, the observations were made on subjective complaint, clinical symptom, and radiographic appearance. Results: The results showed no subjective complaint such as pain or problem on mastication; no negative clinical symptoms such as pain on palpation, gingivitis or periodontitis, and abnormal tooth mobility; no negative radiographic appearance such as pathological apical radioluscency, internal or external resorbtion, and change of ligament periodontal widthafter the treatment. Conclusion: The study suggested that indirect pulp capping treatment using glass ionomer cement materials on primary teeth might be considered to be the treatment choice.Latar belakang: Indirect pulp capping pada gigi sulung lebih jarang dilakukan dibandingkan gigi permanen, karena dianggap memiliki dampak yang rendah dan sebagian besar menyarankan untuk mengambil lesi karies secara agresif pada gigi sulung. Tujuan: Penelitian ini bertujuan

  17. A theranostic dental pulp capping agent with improved MRI and CT contrast and biological properties.

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    Mastrogiacomo, S; Güvener, N; Dou, W; Alghamdi, H S; Camargo, W A; Cremers, J G O; Borm, P J A; Heerschap, A; Oosterwijk, E; Jansen, J A; Walboomers, X F

    2017-10-15

    Different materials have been used for vital dental pulp treatment. Preferably a pulp capping agent should show appropriate biological performance, excellent handling properties, and a good imaging contrast. These features can be delivered into a single material through the combination of therapeutic and diagnostic agents (i.e. theranostic). Calcium phosphate based composites (CPCs) are potentially ideal candidate for pulp treatment, although poor imaging contrast and poor dentino-inductive properties are limiting their clinical use. In this study, a theranostic dental pulp capping agent was developed. First, imaging properties of the CPC were improved by using a core-shell structured dual contrast agent (csDCA) consisting of superparamagnetic iron oxide (SPIO) and colloidal gold, as MRI and CT contrast agent respectively. Second, biological properties were implemented by using a dentinogenic factor (i.e. bone morphogenetic protein 2, BMP-2). The obtained CPC/csDCA/BMP-2 composite was tested in vivo, as direct pulp capping agent, in a male Habsi goat incisor model. Our outcomes showed no relevant alteration of the handling and mechanical properties (e.g. setting time, injectability, and compressive strength) by the incorporation of csDCA particles. In vivo results proved MRI contrast enhancement up to 7weeks. Incisors treated with BMP-2 showed improved tertiary dentin deposition as well as faster cement degradation as measured by µCT assessment. In conclusion, the presented theranostic agent matches the imaging and regenerative requirements for pulp capping applications. In this study, we combined diagnostic and therapeutic agents in order to developed a theranostic pulp capping agent with enhanced MRI and CT contrast and improved dentin regeneration ability. In our study we cover all the steps from material preparation, mechanical and in vitro characterization, to in vivo study in a goat dental model. To the best of our knowledge, this is the first time that a

  18. Direct pulp capping after a carious exposure versus root canal treatment: a cost-effectiveness analysis.

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    Schwendicke, Falk; Stolpe, Michael

    2014-11-01

    Excavation of deep caries often leads to pulpal exposure even in teeth with sensible, nonsymptomatic pulps. Although direct pulp capping (DPC) aims to maintain pulpal health, it frequently requires follow-up treatments like root canal treatment (RCT), which could have been performed immediately after the exposure, with possibly improved outcomes. We quantified and compared the long-term cost-effectiveness of both strategies. A Markov model was constructed following a molar with an occlusally located exposure of a sensible, nonsymptomatic pulp in a 20-year-old male patient over his lifetime. Transition probabilities or hazard functions were estimated based on systematically and nonsystematically assessed literature. Costs were estimated based on German health care, and cost-effectiveness was analyzed using Monte Carlo microsimulations. Despite requiring follow-up treatments significantly earlier, teeth treated by DPC were retained for long periods of time (52 years) at significantly reduced lifetime costs (545 vs 701 Euro) compared with teeth treated by RCT. For teeth with proximal instead of occlusal exposures or teeth in patients >50 years of age, this cost-effectiveness ranking was reversed. Although sensitivity analyses found substantial uncertainty regarding the effectiveness of both strategies, DPC was usually found to be less costly than RCT. We found both DPC and RCT suitable to treat exposed vital, nonsymptomatic pulps. DPC was more cost-effective in younger patients and for occlusal exposure sites, whereas RCT was more effective in older patients or teeth with proximal exposures. These findings might change depending on the health care system and underlying literature-based probabilities. Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  19. Different materials for direct pulp capping: systematic review and meta-analysis and trial sequential analysis.

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    Schwendicke, Falk; Brouwer, Fredrik; Schwendicke, Anja; Paris, Sebastian

    2016-07-01

    We systematically assessed randomized controlled trials comparing direct pulp capping materials. Trials comparing materials for direct capping and evaluating clinically and/or radiographically determined success after minimum 3 months were included. Two reviewers independently screened electronic databases (Medline, Central, Embase) and performed hand searches. Risk of bias was assessed and meta-analyses were performed, separated for dentition. Trial sequential analysis was used to assess risk of random errors. Strength of evidence was graded using the GRADE approach. From a total of 453 identified studies, 11 (all with high risk of bias) investigating 1094 teeth (922 patients) were included. Six studies were on primary teeth (all with carious exposures) and five on permanent teeth (carious and artificial exposures). Mean follow-up was 14 months (range 3-24). Most studies used calcium hydroxide as control, comparing it to mineral trioxide aggregate (MTA) (three studies), bonding without prior etching/conditioning (two), or bonding with prior etching/conditioning, enamel matrix proteins, resin-modified glass ionomer cement, calcium sulfate, zinc oxide eugenol, corticosteroids, antibiotics, or formocresol (each in only one study). One study compared MTA and calcium-enriched cement. In permanent teeth, risk of failure was significantly decreased if MTA instead of calcium hydroxide was used (risk ratio (RR) [95 % confidence intervals (CI)] 0.59 [0.39/0.90]); no difference was found for primary teeth. Other comparisons did not find significant differences or were supported by only one study. No firm evidence was reached according to trial sequential analysis. There is insufficient data to recommend or refute the use of a specific material. More long-term practice-based studies with real-life exposures are required. To reduce risk of failure, dentists might consider using MTA instead of calcium hydroxide (CH) for direct capping. Current evidence is insufficient for

  20. Glycerol Salicylate-based Pulp-Capping Material Containing Portland Cement.

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    Portella, Fernando Freitas; Collares, Fabrício Mezzomo; Santos, Paula Dapper; Sartori, Cláudia; Wegner, Everton; Leitune, Vicente Castelo Branco; Samuel, Susana Maria Werner

    2015-01-01

    The purpose of this study was to evaluate the water sorption, solubility, pH and ability to diffuse into dentin of a glycerol salicylate-based, pulp-capping cement in comparison to a conventional calcium hydroxide-based pulp capping material (Hydcal). An experimental cement was developed containing 60% glycerol salicylate resin, 10% methyl salicylate, 25% calcium hydroxide and 5% Portland cement. Water sorption and solubility were determined based on mass changes in the samples before and after the immersion in distilled water for 7 days. Material discs were stored in distilled water for 24 h, 7 days and 28 days, and a digital pHmeter was used to measure the pH of water. The cement's ability to diffuse into bovine dentin was assessed by Raman spectroscopy. The glycerol salicylate-based cement presented higher water sorption and lower solubility than Hydcal. The pH of water used to store the samples increased for both cements, reaching 12.59 ± 0.06 and 12.54 ± 0.05 after 7 days, for Hydcal and glycerol salicylate-based cements, respectively. Both cements were able to turn alkaline the medium at 24 h and sustain its alkalinity after 28 days. Hydcal exhibited an intense diffusion into dentin up to 40 µm deep, and the glycerol salicylate-based cement penetrated 20 µm. The experimental glycerol salicylate-based cement presents good sorption, solubility, ability to alkalize the surrounding tissues and diffusion into dentin to be used as pulp capping material.

  1. Reactions of human dental pulp cells to capping agents in the presence or absence of bacterial exposure.

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    Cai, Shiwei; Zhang, Wenjian; Tribble, Gena; Chen, Wei

    2017-01-01

    An ideal pulp-capping agent needs to have good biocompatibility and promote reparative dentinogenesis. Although the effects of capping agents on healthy pulp are known, limited data regarding their effects on bacterial contaminated pulp are available. This study aimed to evaluate the reaction of contaminated pulps to various capping agents to assist clinicians in making informed decisions. Human dental pulp (HDP) cell cultures were developed from extracted human molars. The cells were exposed to a bacterial cocktail comprising Porphyromonas gingivalis, Prevotella intermedia, and Streptococcus gordonii before being cocultured with capping agents such as mineral trioxide aggregate (MTA) Portland cement (PC), and Dycal. HDP cell proliferation was assayed by MTS colorimetric cell proliferation assay, and its differentiation was evaluated by real-time PCR for detecting alkaline phosphatase, dentin sialophosphoprotein, and osteocalcin expressions. MTA and PC had no apparent effect, whereas Dycal inhibited HDP cell proliferation. PC stimulated HDP cell differentiation, particularly when they were exposed to bacteria. MTA and Dycal inhibited differentiation, regardless of bacterial infection. In conclusion, PC was the most favorable agent, followed by MTA, and Dycal was the least favorable agent for supporting the functions of bacterial compromised pulp cells.

  2. Biological evaluation of a new pulp capping material developed from Portland cement.

    Science.gov (United States)

    Negm, Ahmed M; Hassanien, Ehab E; Abu-Seida, Ashraf M; Nagy, Mohamed M

    2017-03-02

    This study evaluates the biological properties of a new pulp capping material developed from Portland cement. This study was conducted on 48 teeth in 4 dogs (12 teeth/dog). The dogs were classified into two equal groups (n=24 teeth) according to the evaluation period including: group A (3 weeks) and group B (3 months). Each group was further subdivided into three equal subgroups (n=8 teeth) according to the capping material including: subgroup 1: mineral trioxide aggregate (MTA), subgroup2: Portland cement+10% calcium hydroxide+20% bismuth oxide (Port Cal) and subgroup 3: Portland cement+bismuth oxide. After general anesthesia, a class V buccal cavity was prepared coronal to the gingival margin. After pulp exposure and hemostasis,the capping materials and glass ionomer filling were placed on the exposure sites. All histopathological findings, inflammatory cell count and dentin bridge formation were recorded. Data were analyzed statistically. After 3 months, the histopathological picture of the pulp in subgroup 1 showed normal pulp, continuous odontoblastic layer and complete dentin bridge formation while subgroup 2 showed partial and complete dentin bridge over a normal and necrotic pulps. Subgroup 3 showed loss of normal architecture, areas of necrosis, complete, or incomplete dentin bridge formation, attached and detached pulp stones and fatty degeneration in group B. For group A, MTA subgroup showed the least number of inflammatory cell infiltrate followed by Port Cal subgroup. While subgroup 3 showed the highest number of inflammatory cell infiltrate. For group B, the mean inflammatory cell count increased with the three tested materials with no statistical difference. Regarding dentin bridge formation at group A, no significant differences was found between subgroups, while at group B, MTA subgroup exhibited significantly higher scores than other subgroups. In conclusion, addition of calcium hydroxide to Portland cement improves the dentin bridge formation

  3. Morphological Study Of Border Area Of Pulp-Capping Materials And Er:YAG Laser Prepared Hard Dental Surface

    Directory of Open Access Journals (Sweden)

    Stefanova Vessela P.

    2015-03-01

    Full Text Available Introduction: Vital pulp therapy involves biologically based therapeutic activities aimed at restoring health and preserving the vitality of cariously or traumatically damaged pulp. Adaptation of pulp-capping materials to the prepared tooth surface may be the key to the success of biological tooth treatment.

  4. Nano-hydroxyapatite and calcium-enriched mixture for pulp capping of sound primary teeth: a randomized clinical trial.

    Science.gov (United States)

    Haghgoo, Roza; Asgary, Saeed; Mashhadi Abbas, Fatemeh; Montazeri Hedeshi, Roshanak

    2015-01-01

    Nano-hydroxyapatite (NHA) has been used for regeneration of osseous defects. Calcium-enriched mixture (CEM) cement is also used for various dental treatments. This trial compared the efficacy of NHA and CEM cement for direct pulp capping (DPC) of sound primary teeth. In this randomized clinical trial with split-mouth design, after attaining informed consent, 20 sound primary canines scheduled for orthodontic extraction, were selected. After mechanical pulp exposure, the exposed site was capped with either NHA or CEM cement and then immediately restored with glass-ionomer and resin composite. The teeth were extracted after two months and examined histologically. Parameters of hard tissue bridge (HTB) formation, its type and quality as well as pulpal inflammation scores were compared between the two experimental groups. The data were analyzed using the Mann Whitney U and Fisher's exact test. The level of significance was set at 0.001. All CEM specimens showed inflammation score of 0 (less than 10%). However, in NHA group, inflammation scores of 0 (less than 10%), 1 (10%-30%) and 2 (30%-50%) were observed in 2 (20%), 4 (40%) and 4 (40%) specimens, respectively (P<0.001). HTB was formed in all CEM specimens while it was developed in 2 specimens of NHA (20%; P<0.001). All CEM specimens showed normal pulp; only two cases in NHA group (20%) demonstrated uninflamed normal pulp. CEM cement was superior to NHA as a DPC agent in terms of HTB formation and pulp inflammation scores. It is a suitable material for the DPC of primary teeth.

  5. Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells.

    Science.gov (United States)

    Yoshida, S; Wada, N; Hasegawa, D; Miyaji, H; Mitarai, H; Tomokiyo, A; Hamano, S; Maeda, H

    2016-10-01

    In cases of pulp exposure due to deep dental caries or severe traumatic injuries, existing pulp-capping materials have a limited ability to reconstruct dentin-pulp complexes and can result in pulpectomy because of their low potentials to accelerate dental pulp cell activities, such as migration, proliferation, and differentiation. Therefore, the development of more effective therapeutic agents has been anticipated for direct pulp capping. Dental pulp tissues are enriched with dental pulp stem cells (DPSCs). Here, the authors investigated the effects of semaphorin 3A (Sema3A) on various functions of human DPSCs in vitro and reparative dentin formation in vivo in a rat dental pulp exposure model. Immunofluorescence staining revealed expression of Sema3A and its receptor Nrp1 (neuropilin 1) in rat dental pulp tissue and human DPSC clones. Sema3A induced cell migration, chemotaxis, proliferation, and odontoblastic differentiation of DPSC clones. In addition, Sema3A treatment of DPSC clones increased β-catenin nuclear accumulation, upregulated expression of the FARP2 gene (FERM, RhoGEF, and pleckstrin domain protein 2), and activated Rac1 in DPSC clones. Furthermore, in the rat dental pulp exposure model, Sema3A promoted reparative dentin formation with dentin tubules and a well-aligned odontoblast-like cell layer at the dental pulp exposure site and with novel reparative dentin almost completely covering pulp tissue at 4 wk after direct pulp capping. These findings suggest that Sema3A could play an important role in dentin regeneration via canonical Wnt/β-catenin signaling. Sema3A might be an alternative agent for direct pulp capping, which requires further study. © International & American Associations for Dental Research 2016.

  6. Outcomes of Different Vital Pulp Therapy Techniques on Symptomatic Permanent Teeth: A Case Series

    OpenAIRE

    Asgary, Saeed; Fazlyab, Mahta; Sabbagh, Sedigheh; Eghbal, Mohammad Jafar

    2014-01-01

    In modern endodontics, vital pulp therapy (VPT) has been considered an ultra-conservative treatment modality. Based on the level of pulp preservation, VPT includes stepwise excavation, indirect pulp capping (IDPC), direct pulp capping (DPC), miniature pulpotomy (MP), partial/Cvek pulpotomy and coronal/complete pulpotomy (CP). The present article reviews the treatment outcomes of 94 permanent teeth with irreversible pulpitis treated with either IDPC (n=28), DPC (n=28), MP (n=29) or CP (n=9) us...

  7. Histological evaluation of the rat dental pulp after indirect capping with sildenafil or L-NAME incorporated into a bioadhesive thermoresponsive system

    Directory of Open Access Journals (Sweden)

    Rogério Rodrigues Cupertino

    2016-06-01

    Full Text Available We evaluated the histological dental pulp state in vivo after indirect pulp capping using sildenafil or LG-nitro-L-arginine (L-NAME, incorporated into a new bioadhesive thermoresponsive system (BTS. Male Wistar rats were subjected to an upper and lower first molar class I cavity preparation followed by indirect pulp capping with sildenafil or L-NAME. Calcium hydroxide (CaOH2 was used as a control. The teeth and surrounding bone were properly dissected and processed for Nissl’s staining. Pulp state was evaluated considering the morphological aspects of the inflammatory response, type of inflammatory infiltrate, organization of the odontoblast layer, blood vessel condition, and presence of abscesses or necrosis. The results were expressed as average of observations. The most intense inflammatory response was observed 3 days after the cavity preparation. No identified changes were detected in the dental pulp response of the molars treated with L-NAME compared with those treated with CaOH2. A dual effect was observed in the teeth treated with sildenafil. While low sildenafil concentration (0.015% w w-1 promoted effects comparable to CaOH2, at a higher concentration (0.15% w w-1, sildenafil caused a severe inflammatory response and pulp necrosis. This pioneering suggest that NO pathway activity may be a determinant in the process of dental pulp healing.

  8. Incomplete caries removal and indirect pulp capping in primary molars: a randomized controlled trial.

    Science.gov (United States)

    Bressani, Ana Eliza Lemes; Mariath, Adriela Azevedo Souza; Haas, Alex Nogueira; Garcia-Godoy, Franklin; de Araujo, Fernando Borba

    2013-08-01

    To compare the effect of incomplete caries removal (ICR) and indirect pulp capping (IPC) with calcium hydroxide (CH) or an inert material (wax) on color, consistency and contamination of the remaining dentin of primary molars. This double-blind, parallel-design, randomized controlled trial included 30 children presenting one primary molar with deep caries lesion. Children were randomly assigned after ICR to receive IPC with CH or wax. All teeth were then restored with resin composite. Baseline dentin color and consistency were evaluated after ICR, and dentin samples were collected for contamination analyses using scanning electron microscopy. After 3 months, restorations were removed and the three parameters were re-evaluated. In both groups, dentin became significantly darker after 3 months. No cases of yellow dentin were observed after 3 months with CH compared to 33.3% of the wax cases (P Contamination changed significantly over time in CH and wax without significant difference between groups. It was concluded that CH and wax arrested the carious process of the remaining carious dentin after indirect pulp capping, but CH showed superior dentin color and consistency after 3 months.

  9. Outcomes of different vital pulp therapy techniques on symptomatic permanent teeth: a case series.

    Science.gov (United States)

    Asgary, Saeed; Fazlyab, Mahta; Sabbagh, Sedigheh; Eghbal, Mohammad Jafar

    2014-01-01

    In modern endodontics, vital pulp therapy (VPT) has been considered an ultra-conservative treatment modality. Based on the level of pulp preservation, VPT includes stepwise excavation, indirect pulp capping (IDPC), direct pulp capping (DPC), miniature pulpotomy (MP), partial/Cvek pulpotomy and coronal/complete pulpotomy (CP). The present article reviews the treatment outcomes of 94 permanent teeth with irreversible pulpitis treated with either IDPC (n=28), DPC (n=28), MP (n=29) or CP (n=9) using calcium-enriched mixture (CEM) cement. After a mean follow-up time of 12.3 months, 93 treated teeth were radiographic/clinically successful; only one radiographic failure was observed in the DPC group.

  10. [Vital pulp therapy of damaged dental pulp].

    Science.gov (United States)

    Xuedong, Zhou; Dingming, Huang; Jianguo, Liu; Zhengwei, Huang; Xin, Wei; Deqin, Yang; Jin, Zhao; Liming, Chen; Lin, Zhu; Yanhong, Li; Jiyao, Li

    2017-08-01

    The development of an expert consensus on vital pulp therapy can provide practical guidance for the improvement of pulp damage care in China. Dental pulp disease is a major type of illness that adversely affects human oral health. Pulp capping and pulpotomy are currently the main methods for vital pulp therapy. Along with the development of minimal invasion cosmetic dentistry, using different treatment technologies and materials reasonably, preserving healthy tooth tissue, and extending tooth save time have become urgent problems that call for immediate solution in dental clinics. This paper summarizes the experiences and knowledge of endodontic experts. We develop a clinical path of vital pulp therapy for clinical work by utilizing the nature, approach, and degree of pulp damage as references, defense and self-repairing ability of pulp as guidance, and modern technologies of diagnosis and treatment as means.

  11. Biological response of pulps submitted to different capping materials Resposta biológica de polpas submetidas a diferentes materiais capeadores

    Directory of Open Access Journals (Sweden)

    André Luiz Fraga Briso

    2006-09-01

    Full Text Available Pulp capping is a procedure that comprises adequate protection of the pulp tissue exposed to the oral environment, aiming at the preservation of its vitality and functions. This study evaluated the response of the dental pulps of dog teeth to capping with mineral trioxide aggregate (MTA or calcium hydroxide P.A. For that purpose, 37 teeth were divided into two groups, according to the capping material employed. Two dogs were anesthetized and, after placement of a rubber dam, their pulps were exposed in a standardized manner and protected with the experimental capping materials. The cavities were then sealed with resin-modified glass ionomer cement and restored with composite resin. After sixty days, the animals were killed and the specimens were processed in order to be analyzed with optic microscopy. It was observed that MTA presented a higher success rate compared to calcium hydroxide, presenting a lower occurrence of infection and pulp necrosis.O capeamento pulpar é um procedimento que consiste na adequada proteção do tecido pulpar exposto ao meio oral, objetivando a preservação de sua vitalidade e suas funções. O objetivo deste trabalho foi observar a resposta da polpa dental de cães ao capeamento com o agregado de trióxido mineral (MTA ou hidróxido de cálcio P.A. Para tanto, 37 dentes foram divididos em dois grupos, de acordo com o material capeador utilizado. Dois cães foram anestesiados e após o isolamento absoluto do campo operatório, realizou-se exposição padronizada da polpa, proteção com os materiais capeadores testados e selamento das cavidades com cimento modificado de ionômero de vidro. Os dentes foram então restaurados com resina composta. Após sessenta dias, os animais foram sacrificados e as peças foram processadas para a análise microscópica dos dentes. Observou-se que o MTA apresentou maior índice de sucesso em relação ao hidróxido de cálcio, evidenciando menor ocorrência de infecção e necrose

  12. MTA and calcium hydroxide for pulp capping MTA e hidróxido de cálcio para proteção pulpar direta

    Directory of Open Access Journals (Sweden)

    Alexandra Mussolino de Queiroz

    2005-06-01

    Full Text Available This study evaluated the biocompatibility of mineral trioxide aggregate (MTA after direct capping of exposed pulp tissue in dog's teeth. Class I cavities were prepared in 26 teeth from 3 adult dogs. MTA was applied over the exposed pulp in 13 teeth and paste of calcium hydroxide plus distilled water (control was applied in the remaining 13 teeth. After 90 days, the animals were killed; the maxilla and mandible were dissected and sectioned to obtain individual roots. The samples were processed histologically. The pulp and periapical response observed with the use of MTA was similar to that of calcium hydroxide paste. In all specimens, there was a dentin bridge obliterating the exposure, an intact odontoblastic layer, no inflammatory cells, normal connective pulp tissue, normal apical and periapical regions and no bone tissue changes. Similar to calcium hydroxide, MTA presented excellent response when used for direct pulp capping.O objetivo deste trabalho foi avaliar a biocompatibilidade do agregado de trióxido mineral (MTA, após proteção pulpar direta em dentes de cães. Foram preparadas cavidades de Classe I, em 26 dentes de 3 cães adultos. O MTA foi aplicado sobre 13 dentes e a pasta de hidróxido de cálcio (grupo controle foi aplicada sobre os 13 dentes remanescentes. Após 90 dias, os animais foram mortos, a maxila e a mandíbula foram dissecadas e os dentes foram seccionados para obtenção de raízes individualizadas. Os espécimes foram processados histologicamente. A resposta do tecido pulpar e periapical foi semelhante para o MTA e o hidróxido de cálcio. Em todos os espécimes havia ponte de dentina obliterando o local da exposição pulpar, camada odontoblástica íntegra, ausência de células inflamatórias, tecido pulpar normal, e ausência de alterações na região periapical e óssea. Da mesma maneira que o hidróxido de cálcio, o MTA apresentou excelente biocompatibilidade quando usado para proteção pulpar direta.

  13. Is Pulp Inflammation a Prerequisite for Pulp Healing and Regeneration?

    Directory of Open Access Journals (Sweden)

    Michel Goldberg

    2015-01-01

    Full Text Available The importance of inflammation has been underestimated in pulpal healing, and in the past, it has been considered only as an undesirable effect. Associated with moderate inflammation, necrosis includes pyroptosis, apoptosis, and nemosis. There are now evidences that inflammation is a prerequisite for pulp healing, with series of events ahead of regeneration. Immunocompetent cells are recruited in the apical part. They slide along the root and migrate toward the crown. Due to the high alkalinity of the capping agent, pulp cells display mild inflammation, proliferate, and increase in number and size and initiate mineralization. Pulp fibroblasts become odontoblast-like cells producing type I collagen, alkaline phosphatase, and SPARC/osteonectin. Molecules of the SIBLING family, matrix metalloproteinases, and vascular and nerve mediators are also implicated in the formation of a reparative dentinal bridge, osteo/orthodentin closing the pulp exposure. Beneath a calciotraumatic line, a thin layer identified as reactionary dentin underlines the periphery of the pulp chamber. Inflammatory and/or noninflammatory processes contribute to produce a reparative dentinal bridge closing the pulp exposure, with minute canaliculi and large tunnel defects. Depending on the form and severity of the inflammatory and noninflammatory processes, and according to the capping agent, pulp reactions are induced specifically.

  14. Uso de novos materiais para o capeamento pulpar (hidroxiapatita - HAp e fosfato tricálcico - β-TCP The use of new materials for pulp capping (hydroxyapatite - HAp and tricalcium phosphate - β-TCP

    Directory of Open Access Journals (Sweden)

    C. S. Delfino

    2010-12-01

    Full Text Available O capeamento pulpar é uma medida importante e muito usada no cotidiano da prática odontológica e, quando realizado de forma consciente, baseado em um bom diagnóstico da condição pulpar no momento da exposição, pode prevenir o dente de sofrer uma intervenção endodôntica. Além do hidróxido de cálcio, outros materiais vêm sendo testados quanto a sua ação em promover o reparo pulpar pela formação de ponte de dentina, após capeamento pulpar direto. Uma das alternativas de materiais viáveis para o capeamento pulpar são as cerâmicas à base de fosfatos de cálcio, destacando-se a hidroxiapatita (HAp e o fosfato tricálcico (β-TCP. Estes materiais não promovem a formação de área necrótica, característica da utilização do hidróxido de cálcio, são biocompatíveis e favorecem o reparo do tecido pulpar. Para avaliar os efeitos desses materiais no capeamento pulpar foi realizada esta revisão de literatura, abordando desde a resposta do tecido pulpar, até o protocolo para o uso clínico.Pulp capping is an important measure and one with a high rate of use in daily dental practice, and when it is conscientiously performed, based on good diagnosis of the pulp condition at the time of its exposure, it may prevent the tooth from requiring endodontic intervention. In addition to calcium hydroxide, other materials have been tested with regard to their action in promoting pulp repair by the formation of a dentin bridge after direct pulp capping. Some of the feasible alternative materials for pulp capping are calcium phosphate-based ceramics, with hydroxyapatite (HAp and tricalcium phosphate (β-TCP being outstanding. These materials do not promote formation of a necrotic area, characteristic of the use of calcium hydroxide, are biocompatible and favor pulp tissue repair. To evaluate the effects of these materials on pulp capping, this literature review was conducted, the approach being wide ranging in scope, from pulp response

  15. Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells.

    Science.gov (United States)

    Serita, Suguru; Tomokiyo, Atsushi; Hasegawa, Daigaku; Hamano, Sayuri; Sugii, Hideki; Yoshida, Shinichiro; Mizumachi, Hiroyuki; Mitarai, Hiromi; Monnouchi, Satoshi; Wada, Naohisa; Maeda, Hidefumi

    2017-06-01

    The aim of this study was to investigate transforming growth factor-β-induced gene product-h3 (βig-h3) expression in dental pulp tissue and its effects on odontoblastic differentiation of dental pulp cells (DPCs). A rat direct pulp capping model was prepared using perforated rat upper first molars capped with mineral trioxide aggregate cement. Human DPCs (HDPCs) were isolated from extracted teeth. βig-h3 expression in rat dental pulp tissue and HDPCs was assessed by immunostaining. Mineralization of HDPCs was assessed by Alizarin red-S staining. Odontoblast-related gene expression in HDPCs was analyzed by quantitative RT-PCR. Expression of βig-h3 was detected in rat dental pulp tissue, and attenuated by direct pulp capping, while expression of interleukin-1β and tumor necrosis factor-α was increased in exposed pulp tissue. βig-h3 expression was also detected in HDPCs, with reduced expression during odontoblastic differentiation. The above cytokines reduced βig-h3 expression in HDPCs, and promoted their mineralization. Recombinant βig-h3 inhibited the expression of odontoblast-related genes and mineralization of HDPCs, while knockdown of βig-h3 gene expression promoted the expression of odontoblast-related genes in HDPCs. The present findings suggest that βig-h3 in DPCs may be involved in reparative dentin formation and that its expression is likely to negatively regulate this process. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. The Biomineralization of a Bioactive Glass-Incorporated Light-Curable Pulp Capping Material Using Human Dental Pulp Stem Cells

    Directory of Open Access Journals (Sweden)

    Soo-Kyung Jun

    2017-01-01

    Full Text Available The aim of this study was to investigate the biomineralization of a newly introduced bioactive glass-incorporated light-curable pulp capping material using human dental pulp stem cells (hDPSCs. The product (Bioactive® [BA] was compared with a conventional calcium hydroxide-incorporated (Dycal [DC] and a light-curable (Theracal® [TC] counterpart. Eluates from set specimens were used for investigating the cytotoxicity and biomineralization ability, determined by alkaline phosphatase (ALP activity and alizarin red staining (ARS. Cations and hydroxide ions in the extracts were measured. An hDPSC viability of less than 70% was observed with 50% diluted extract in all groups and with 25% diluted extract in the DC. Culturing with 12.5% diluted BA extract statistically lowered ALP activity and biomineralization compared to DC (p0.05. Ca (~110 ppm and hydroxide ions (pH 11 were only detected in DC and TC. Ionic supplement-added BA, which contained similar ion concentrations as TC, showed similar ARS mineralization compared to TC. In conclusion, the BA was similar to, yet more cytotoxic to hDPSCs than, its DC and TC. The BA was considered to stimulate biomineralization similar to DC and TC only when it released a similar amount of Ca and hydroxide ions.

  17. Clinical e radiographic sucess of indirect pulp capping with partial excavation of demineralization dentin in primary posterior teeth

    Directory of Open Access Journals (Sweden)

    Roberta Francisca Martins de Castro

    2009-10-01

    Full Text Available Objective: To assess the outcome of the indirect pulp capping technique after incomplete excavation of the demineralized dentin in primary molars of patients treated in the period from 2004 to 2006.Methods: Sample consisted of 81 teeth of 36 patients aged from 1-to-8 year-old. Clinical and radiographic inclusion criteria, as well as criteria of the success of the evaluated treatments were established.Results: There were four (4.9% failures and 77 (95.1% successful treatments. Treatment failure occurred in three girls and in one boy, and no statistically significant difference (p=0.341 was found. Twenty three teeth were treated with resin-modified glass-ionomer cement and 58 with composite resin, of which 22 and 55, respectively, showed successful results. There was no statistically significant difference between restorative materials used (p=0.877. As regards the studied age group, patients aged from 1 to 4 years showed a significantly lower success rate when compared with 5-8 year-old children (p = 0.0428. Conclusion: The results suggest that the indirect pulp capping technique after incomplete excavation of demineralized dentin is a reliable technique to be adopted in deep lesions of primary teeth.

  18. Clinical and Radiographic Assessment of the Efficacy of Calcium Silicate Indirect Pulp Capping

    Science.gov (United States)

    Hashem, D.; Mannocci, F.; Patel, S.; Manoharan, A.; Brown, J.E.; Watson, T.F.

    2015-01-01

    The aims of this study were to assess the effectiveness of calcium silicate cement (Biodentine) versus glass ionomer cement (GIC; control group) as indirect pulp capping materials in patients with reversible pulpitis and to compare the effectiveness of cone beam computed tomography (CBCT) versus periapical (PA) radiographs in detecting PA changes at baseline (T0) and at 12 mo (T12) postoperatively. Seventy-two restorations (36 Biodentine, 36 Fuji IX) were placed randomly in 53 patients. CBCT/PA radiographs were taken at T0 and T12. Two calibrated examiners assessed the presence/absence and increase/decrease in the size of existing PA radiolucencies under standardized conditions. The Kappa coefficient evaluated statistically the effectiveness of CBCT versus PA radiographs in detecting PA changes. Chi-square/Mann-Whitney tests were used to evaluate the association between PA changes in CBCT with various clinical measures. Significance was predetermined at α = 0.05. Clinical success rates for Biodentine and Fuji IX GIC were 83.3%. CBCT was significantly more effective in detecting PA radiolucencies compared with radiographs (P = 0.0069). Of the teeth, 65.4% and 90.4% were deemed healthy using CBCT and PA radiographs, respectively, at T12. Healing/healed rates were 17.3%/0%, while new/progressed radiolucency were 30.8%/9.6% with CBCT/PA radiographs, respectively. Seventy-one percent of healed lesions had received Biodentine; 88% of new/progressed lesions received Fuji IX GIC. Teeth presenting with an initial CBCT PA lesion had a failure rate of 63%, whereas teeth with no initial lesion had a failure rate of 16%. Although no statistically significant difference was detected in the clinical efficacy of Biodentine/Fuji IX when used as indirect pulp capping materials in patients with reversible pulpitis, CBCT showed a significant difference in that most healed CBCT lesions had received Biodentine while most that did not heal received Fuji IX. Longer-term follow-up is

  19. The annual frequency of root fillings, tooth extractions and pulp-related procedures in Danish adults during 1977-2003

    DEFF Research Database (Denmark)

    Bjørndal, L.; Reit, C.

    2004-01-01

    endodontic epidemiology, frequency of root fillings, pulp capping, stepwise excavation, tooth extractions......endodontic epidemiology, frequency of root fillings, pulp capping, stepwise excavation, tooth extractions...

  20. HIGHLY ENERGY EFFICIENT D-GLU (DIRECTED-GREEN LIQ-UOR UTILIZATION) PULPING

    Energy Technology Data Exchange (ETDEWEB)

    Lucia, Lucian A

    2013-04-19

    Purpose: The purpose of the project was to retrofit the front end (pulp house) of a commercial kraft pulping mill to accommodate a mill green liquor (GL) impregna-tion/soak/exposure and accrue downstream physical and chemical benefits while prin-cipally reducing the energy footprint of the mill. A major player in the mill contrib-uting to excessive energy costs is the lime kiln. The project was intended to offload the energy (oil or natural gas) demands of the kiln by by-passing the causticization/slaking site in the recovery area and directly using green liquor as a pulping medium for wood. Scope: The project was run in two distinct, yet mutually compatible, phases: Phase 1 was the pre-commercial or laboratory phase in which NC State University and the Insti-tute of Paper Science and Technology (at the Georgia Institute of Technology) ran the pulping and associated experiments, while Phase 2 was the mill scale trial. The first tri-al was run at the now defunct Evergreen Pulp Mill in Samoa, CA and lead to a partial retrofit of the mill that was not completed because it went bankrupt and the work was no longer the low-hanging fruit on the tree for the new management. The second trial was run at the MeadWestvaco Pulp Mill in Evedale, TX which for all intents and pur-poses was a success. They were able to fully retrofit the mill, ran the trial, studied the pulp properties, and gave us conclusions.

  1. Mill Integration-Pulping, Stream Reforming and Direct Causticization for Black Liquor Recovery

    Energy Technology Data Exchange (ETDEWEB)

    Adriaan van Heiningen

    2007-06-30

    MTCI/StoneChem developed a steam reforming, fluidized bed gasification technology for biomass. DOE supported the demonstration of this technology for gasification of spent wood pulping liquor (or 'black liquor') at Georgia-Pacific's Big Island, Virginia mill. The present pre-commercial R&D project addressed the opportunities as well as identified negative aspects when the MTCI/StoneChem gasification technology is integrated in a pulp mill production facility. The opportunities arise because black liquor gasification produces sulfur (as H{sub 2}S) and sodium (as Na{sub 2}CO{sub 3}) in separate streams which may be used beneficially for improved pulp yield and properties. The negative aspect of kraft black liquor gasification is that the amount of Na{sub 2}CO{sub 3} which must be converted to NaOH (the so called causticizing requirement) is increased. This arises because sulfur is released as Na{sub 2}S during conventional kraft black liquor recovery, while during gasification the sodium associated Na{sub 2}S is partly or fully converted to Na{sub 2}CO{sub 3}. The causticizing requirement can be eliminated by including a TiO{sub 2} based cyclic process called direct causticization. In this process black liquor is gasified in the presence of (low sodium content) titanates which convert Na{sub 2}CO{sub 3} to (high sodium content) titanates. NaOH is formed when contacting the latter titanates with water, thereby eliminating the causticizing requirement entirely. The leached and low sodium titanates are returned to the gasification process. The project team comprised the University of Maine (UM), North Carolina State University (NCSU) and MTCI/ThermoChem. NCSU and MTCI are subcontractors to UM. The principal organization for the contract is UM. NCSU investigated the techno-economics of using advanced pulping techniques which fully utilize the unique cooking liquors produced by steam reforming of black liquor (Task 1). UM studied the kinetics and

  2. Exogenous nitric oxide stimulates the odontogenic differentiation of rat dental pulp stem cells.

    Science.gov (United States)

    Sonoda, Soichiro; Mei, Yu-Feng; Atsuta, Ikiru; Danjo, Atsushi; Yamaza, Haruyoshi; Hama, Shion; Nishida, Kento; Tang, Ronghao; Kyumoto-Nakamura, Yukari; Uehara, Norihisa; Kukita, Toshio; Nishimura, Fusanori; Yamaza, Takayoshi

    2018-02-21

    Nitric oxide (NO) is thought to play a pivotal regulatory role in dental pulp tissues under both physiological and pathological conditions. However, little is known about the NO functions in dental pulp stem cells (DPSCs). We examined the direct actions of a spontaneous NO gas-releasing donor, NOC-18, on the odontogenic capacity of rat DPSCs (rDPSCs). In the presence of NOC-18, rDPSCs were transformed into odontoblast-like cells with long cytoplasmic processes and a polarized nucleus. NOC-18 treatment increased alkaline phosphatase activity and enhanced dentin-like mineralized tissue formation and the expression levels of several odontoblast-specific genes, such as runt related factor 2, dentin matrix protein 1 and dentin sialophosphoprotein, in rDPSCs. In contrast, carboxy-PTIO, a NO scavenger, completely suppressed the odontogenic capacity of rDPSCs. This NO-promoted odontogenic differentiation was activated by tumor necrosis factor-NF-κB axis in rDPSCs. Further in vivo study demonstrated that NOC-18-application in a tooth cavity accelerated tertiary dentin formation, which was associated with early nitrotyrosine expression in the dental pulp tissues beneath the cavity. Taken together, the present findings indicate that exogenous NO directly induces the odontogenic capacity of rDPSCs, suggesting that NO donors might offer a novel host DPSC-targeting alternative to current pulp capping agents in endodontics.

  3. SUCCESS RATE OF ONE SESSION AND TWO SESSION TECHNIQUES FOR TREATMENT OF ASYMPTOMATIC PULPITIS OF PRIMARY TEETH WITH INDIRECT PULP CAPPING.

    Directory of Open Access Journals (Sweden)

    Rossitza Kabaktchieva

    2013-01-01

    Full Text Available Objective: Objective: To compare the success rate between the one session and two session indirect pulp capping of asymptomatic pulpitis for 1 year after the treatment was performed in children with different caries risk. Material and Methods: The children and the teeth were selected according to certain criteria for inclusion in the study. According to that, 72 children with low, moderate and high caries risk were included and 131 teeth with asymptomatic pulpitis were treated. The clinical protocols for indirect pulp capping (IPC in one session and two sessions were defined. The review appointments were performed 6 and 12 months after the treatment using certain clinical and radiographic criteria which defined success or post-treatment complications. The results are statistically analysed using One Sided Exact Two-Proportion Test with 95% Significance level (5% risk of type I error. Results: The statistical test showed that between the compared success rates of the one session and two sessions IPC, on the 6th and 12th month, there wasn’t a significant difference (p>0.05. This was valid for every one of the examined groups of patients (p>0. 05. This proves that there is no difference in the success rates of treatment of pulpitis in primary teeth using one or two sessions. Conclusions: The results about the success of treatment of asymptomatic pulpitis in primary teeth during one or two sessions have confirmed the results showed in other current studies. Our study has confirmed (clinically and radiographically the success of both techniques and we have concluded that in children with high caries risk, more appropriate technique is the one performed in two sessions. Treatment in one session is recommended in children with low or moderate caries risk.

  4. Bi-directional Multi Dimension CAP Transmission for Smart Grid Communication Services

    DEFF Research Database (Denmark)

    Zhang, Xu; Binti Othman, Maisara; Pang, Xiaodan

    2012-01-01

    We experimentally demonstrate bi-directional multi dimension carrierless amplitude and phase (CAP) transmission for smart grid communication services based on optical fiber networks. The proposed system is able to support multi-Gb/s transmission with high spectral efficiency.......We experimentally demonstrate bi-directional multi dimension carrierless amplitude and phase (CAP) transmission for smart grid communication services based on optical fiber networks. The proposed system is able to support multi-Gb/s transmission with high spectral efficiency....

  5. Arundo donax L. reed: new perspectives for pulping and bleaching. Part 4. Peroxide bleaching of organosolv pulps.

    Science.gov (United States)

    Shatalov, A A; Pereira, H

    2005-05-01

    A comparative study on TCF (totally chlorine-free) bleachability of organosolv pulps from the annual fibre crop Arundo donax L. (giant reed) was carried out using a simple three-stage peroxide bleaching sequence without oxygen pre-bleaching. ASAM (alkali-sulfite-anthraquinone-methanol), Organocell (alkali-anthraquinone-methanol) and ethanol-soda organosolv pulps were bleached and compared with kraft pulp, as a reference. The final brightness of 76-78% ISO was attained for all tested pulps. The chemical charge required to reach this level of brightness varied for different pulps (despite the equal initial content of the residual lignin) and directly related to starting brightness values. No direct correlation between brightness improvement and lignin removal during bleaching was found, indicating the influence of the specific pulp properties introduced by pulping process on bleaching chemistry. The general higher bleaching response of organosolv pulps from A. donax was noted in comparison with kraft.

  6. Nemesia root hair response to paper pulp substrate for micropropagation.

    Science.gov (United States)

    Labrousse, Pascal; Delmail, David; Decou, Raphaël; Carlué, Michel; Lhernould, Sabine; Krausz, Pierre

    2012-01-01

    Agar substrates for in vitro culture are well adapted to plant micropropagation, but not to plant rooting and acclimatization. Conversely, paper-pulp-based substrates appear as potentially well adapted for in vitro culture and functional root production. To reinforce this hypothesis, this study compares in vitro development of nemesia on several substrates. Strong differences between nemesia roots growing in agar or in paper-pulp substrates were evidenced through scanning electron microscopy. Roots developed in agar have shorter hairs, larger rhizodermal cells, and less organized root caps than those growing on paper pulp. In conclusion, it should be noted that in this study, in vitro microporous substrates such as paper pulp lead to the production of similar root hairs to those found in greenhouse peat substrates. Consequently, if agar could be used for micropropagation, rooting, and plant acclimatization, enhancement could be achieved if rooting stage was performed on micro-porous substrates such as paper pulp.

  7. Direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and β-glucosidase.

    Science.gov (United States)

    Apiwatanapiwat, Waraporn; Murata, Yoshinori; Kosugi, Akihiko; Yamada, Ryosuke; Kondo, Akihiko; Arai, Takamitsu; Rugthaworn, Prapassorn; Mori, Yutaka

    2011-04-01

    In order to develop a method for producing fuel ethanol from cassava pulp using cell surface engineering (arming) technology, an arming yeast co-displaying α-amylase (α-AM), glucoamylase, endoglucanase, cellobiohydrase, and β-glucosidase on the surface of the yeast cells was constructed. The novel yeast strain, possessing the activities of all enzymes, was able to produce ethanol directly from soluble starch, barley β-glucan, and acid-treated Avicel. Cassava is a major crop in Southeast Asia and used mainly for starch production. In the starch manufacturing process, large amounts of solid wastes, called cassava pulp, are produced. The major components of cassava pulp are starch (approximately 60%) and cellulose fiber (approximately 30%). We attempted simultaneous saccharification and ethanol fermentation of cassava pulp with this arming yeast. During fermentation, ethanol concentration increased as the starch and cellulose fiber substrates contained in the cassava pulp decreased. The results clearly showed that the arming yeast was able to produce ethanol directly from cassava pulp without addition of any hydrolytic enzymes.

  8. Direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and {beta}-glucosidase

    Energy Technology Data Exchange (ETDEWEB)

    Apiwatanapiwat, Waraporn; Rugthaworn, Prapassorn [Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki (Japan). Post-Harvest Science and Technology Div.; Kasetsart Univ., Bangkok (Thailand). Nanotechnology and Biotechnology Div.; Murata, Yoshinori; Kosugi, Akihiko; Arai, Takamitsu; Mori, Yutaka [Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki (Japan). Post-Harvest Science and Technology Div.; Yamada, Ryosuke; Kondo, Akihiko [Kobe Univ. (Japan). Dept. of Chemical Science and Engineering

    2011-04-15

    In order to develop a method for producing fuel ethanol from cassava pulp using cell surface engineering (arming) technology, an arming yeast co-displaying {alpha}-amylase ({alpha}-AM), glucoamylase, endoglucanase, cellobiohydrase, and {beta}-glucosidase on the surface of the yeast cells was constructed. The novel yeast strain, possessing the activities of all enzymes, was able to produce ethanol directly from soluble starch, barley {beta}-glucan, and acid-treated Avicel. Cassava is a major crop in Southeast Asia and used mainly for starch production. In the starch manufacturing process, large amounts of solid wastes, called cassava pulp, are produced. The major components of cassava pulp are starch (approximately 60%) and cellulose fiber (approximately 30%). We attempted simultaneous saccharification and ethanol fermentation of cassava pulp with this arming yeast. During fermentation, ethanol concentration increased as the starch and cellulose fiber substrates contained in the cassava pulp decreased. The results clearly showed that the arming yeast was able to produce ethanol directly from cassava pulp without addition of any hydrolytic enzymes. (orig.)

  9. Experimental Investigations of 3-D-/4-D-CAP Modulation With Directly Modulated VCSELs

    DEFF Research Database (Denmark)

    Binti Othman, Maisara; Zhang, Xu; Deng, Lei

    2012-01-01

    correction limit of 2.8 × 10-3 for error-free reception is achieved after 20 km of SSMF transmission. Spectral efficiencies of 2.68 and 2.08 b/s/Hz are reported for 3-D-CAP and 4-D-CAP, respectively. We believe that multidimensional modulation formats represent an attractive solution for providing more......In this letter, we present experimental investigations of multidimensional multilevel carrierless amplitude phase (CAP) modulation with directly modulated vertical cavity surface-emitting lasers. The signals are transmitted over 20 km of standard single-mode fiber (SSMF). For multilevel 3-D...

  10. In vitro antibacterial activity of a novel resin-based pulp capping material containing the quaternary ammonium salt MAE-DB and Portland cement.

    Science.gov (United States)

    Yang, Yanwei; Huang, Li; Dong, Yan; Zhang, Hongchen; Zhou, Wei; Ban, Jinghao; Wei, Jingjing; Liu, Yan; Gao, Jing; Chen, Jihua

    2014-01-01

    promise as a pulp capping material for vital pulp preservation in the treatment of deep caries.

  11. Clinical and Radiographic Evaluation of Allium sativum Oil as a New Medicament for Vital Pulp Treatment of Primary Teeth.

    Science.gov (United States)

    Mohammad, Shukry Gamal; Raheel, Syed Ahmed; Baroudi, Kusai

    2014-01-01

    The objective of this study was to compare between the clinical and radiographic effects of Allium sativum oil and those of formocresol in vital pulpotomy in primary teeth. A total of 20 children age ranged from 4 to 8 years were included in the study. In every one of those children, the primary molars indicated for pulpotomy. Pulpotomy procedure was performed, and the radicular pulp tissue of one molar capped with A. sativum oil in a cotton pellet, whereas the other molar capped with formocresol, the teeth evaluated clinically and radiographically before and after 6 months, using standard clinical and radiographical criteria. Statistically, these results revealed no significant difference between the radiographic findings of vital pulpotomy in primary molars with the two medicaments was found. A. sativum oil offers a good healing potential, leaving the remaining pulp tissue healthy and functioning. Vital pulpotomy with allium sativa oil was given raise 90% success rate while that with formocresol was 85%. A. sativum oil is a biocompatible material that is compatible with vital human pulp tissue. It offers a good healing potential, leaving the remaining pulp tissue healthy and functioning.

  12. Ranking mechanical pulps for their potential to photoyellow

    Science.gov (United States)

    Umesh P. Agarwal

    2000-01-01

    Recently found experimental evidence has provided strong support for an alternative photoyellowing mechanism that suggests that pulp- photoyellowing occurs due to direct photooxidation of hydroquinones (present in mechanical pulps) top-quinones. Because hydroquinones were found to be present in pulps, it may be possible to quantify them. Quantification of mechanical-...

  13. Economic and regional consequences of direct payments under the current CAP philosophy

    Directory of Open Access Journals (Sweden)

    Věra Bečvářová

    2011-01-01

    Full Text Available The paper deals with one of the key instruments of the actual EU Common Agricultural Policy (the CAP, the direct payments. The economic as well as the regional implications of application for direct payments in the CAP strategy are evaluated there. The study deals with the economic costs, direct and indirect effects of such type of subsidy in general and a demonstration thereof in the context of the development of the European model of agriculture. Based on the economic principle of this type of transfer evalution, the processes of the income redistribution in the framework of global/common policy in general is characterized. It explains the causes as well as the nature of implementation of this instrument in the reform processes in the last twenty years, focusing on the reasons and consequences of different application in the original and the new EU member states. It considers the question of whether this type of support to agriculture is truly a tool that does not interfere with market conditions affect the respective competitive ability of agricultural producers. In the context of changes to income support in agriculture in the CAP development process, the effect of decoupled payments on the pricing within the agricultural commodity markets is specified. At the same time the work deals with a broader social context and social impact of the differing forms of support in European regions.

  14. Transparent organic light-emitting diodes with different bi-directional emission colors using color-conversion capping layers

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jonghee, E-mail: jonghee.lee@etri.re.kr [OLED Research Center, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Institut für Angewandte Photophysik, Technische Universität Dresden, George-Bähr-Straße 1, 01062 Dresden (Germany); Koh, Tae-Wook [Department of Electrical Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701 (Korea, Republic of); Cho, Hyunsu [OLED Research Center, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Department of Electrical Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701 (Korea, Republic of); Schwab, Tobias [Institut für Angewandte Photophysik, Technische Universität Dresden, George-Bähr-Straße 1, 01062 Dresden (Germany); Lee, Jae-Hyun [Department School of Global Convergence Studies, Hanbat National University, San 16-1, Duckmyoung-dong, Daejeon 305-719 (Korea, Republic of); Hofmann, Simone [Institut für Angewandte Photophysik, Technische Universität Dresden, George-Bähr-Straße 1, 01062 Dresden (Germany); Lee, Jeong-Ik [OLED Research Center, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Yoo, Seunghyup [Department of Electrical Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701 (Korea, Republic of); and others

    2015-06-15

    We report a study on transparent organic light-emitting diodes (OLEDs) with different bi-directional emission colors, enabled by color-conversion organic capping layers. Starting from a transparent blue OLED with an uncapped Ag top electrode exhibiting an average transmittance of 33.9%, a 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran (DCM)-doped tris-(8-hydroxy-quinolinato)-aluminum (Alq3) capping layer is applied to achieve color-conversion from blue to orange-red on the top side while maintaining almost unchanged device transmittance. This color-conversion capping layer does not only change the color of the top side emission, but also enhances the overall device efficiency due to the optical interaction of the capping layer with the primary blue transparent OLED. Top white emission from the transparent bi-directional OLED exhibits a correlated color temperature around 6000–7000 K, with excellent color stability as evidenced by an extremely small variation in color coordinate of Δ(x,y)=(0.002, 0.002) in the forward luminance range of 100–1000 cd m{sup −2}. At the same time, the blue emission color of bottom side is not influenced by the color conversion capping layer, which finally results in different emission colors of the two opposite sides of our transparent OLEDs. - Highlights: • We report transparent organic light-emitting diodes (OLEDs) with different bi-directional emission colors. • Transparent blue OLED with color-conversion organic capping layers (CCL) shows orange top side emission. • Top white emission exhibits a CCT around 7000 K, with excellent color stability on a driving voltage.

  15. Transparent organic light-emitting diodes with different bi-directional emission colors using color-conversion capping layers

    International Nuclear Information System (INIS)

    Lee, Jonghee; Koh, Tae-Wook; Cho, Hyunsu; Schwab, Tobias; Lee, Jae-Hyun; Hofmann, Simone; Lee, Jeong-Ik; Yoo, Seunghyup

    2015-01-01

    We report a study on transparent organic light-emitting diodes (OLEDs) with different bi-directional emission colors, enabled by color-conversion organic capping layers. Starting from a transparent blue OLED with an uncapped Ag top electrode exhibiting an average transmittance of 33.9%, a 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran (DCM)-doped tris-(8-hydroxy-quinolinato)-aluminum (Alq3) capping layer is applied to achieve color-conversion from blue to orange-red on the top side while maintaining almost unchanged device transmittance. This color-conversion capping layer does not only change the color of the top side emission, but also enhances the overall device efficiency due to the optical interaction of the capping layer with the primary blue transparent OLED. Top white emission from the transparent bi-directional OLED exhibits a correlated color temperature around 6000–7000 K, with excellent color stability as evidenced by an extremely small variation in color coordinate of Δ(x,y)=(0.002, 0.002) in the forward luminance range of 100–1000 cd m −2 . At the same time, the blue emission color of bottom side is not influenced by the color conversion capping layer, which finally results in different emission colors of the two opposite sides of our transparent OLEDs. - Highlights: • We report transparent organic light-emitting diodes (OLEDs) with different bi-directional emission colors. • Transparent blue OLED with color-conversion organic capping layers (CCL) shows orange top side emission. • Top white emission exhibits a CCT around 7000 K, with excellent color stability on a driving voltage

  16. An experimental study on healing of pulp wound following pulpotomy in dogs by autoradiography with tritiated thymidine

    International Nuclear Information System (INIS)

    Chen, Chao-How

    1978-01-01

    This experiment is an autoradiographical study of the healing process in pulp wounds following pulpotomy and direct capping with calcium hydroxide. Experimental, young adult, mongrel dogs were divided into the following two groups: A group: Pulpotomy was performed and the following intervals allowed to lapse between amputation and sacrifice. One hour before sacrifice, 1μCi/gbw 3 H-thymidine was injected intravenously (flash labeling). B group: Single pulse labeling was performed either one day (1 spl) or two days (2 spl) after amputation. During the first two days after amputation, pulp stem cells under the cutting surface divided and proliferated, with the proliferative rate reaching a maximum during the second day and decreased gradually from the fourth day after pulpotomy. Cells in the first zone became columnar in shapes and assumed a regular arrangement under the necrotic layer. Though these cells were unlabeled in the A group, they were prominently labeled in the B group. These finding indicate a part of the proliferated undifferentiated mesenchymal cells migrated and regularly arranged beneath the necrotic layer, differentiated into odontoblasts and then produced a dentine bridge. The remaining undifferentiated mesenchymal cells in the second zone reverted to pulp stem cells to accomplish healing. (auth.)

  17. Direct heat transfer considerations for improving energy efficiency in pulp and paper Kraft mills

    International Nuclear Information System (INIS)

    Savulescu, Luciana Elena; Alva-Argaez, Alberto

    2008-01-01

    The success of any process improvement study depends on the quality of the available data and the way in which the plant-specific characteristics are incorporated in the applied conceptual models; in the context of process integration studies these issues are directly related to the rules followed during the data extraction stage. Improving energy efficiency in a pulp and paper Kraft mill requires the identification of the most promising heat recovery network retrofit projects. In a retrofit analysis using pinch technology/process integration methods, only the process streams associated to the existing heat exchangers and some outlet streams (such as wastewater/effluent streams and vents) with high potential for heat recovery are usually included, while the energy exchanged through non-isothermal stream mixing (NIM) or direct heat transfer (DHT) is often assumed fixed and is not considered in the analysis. Relaxing this assumption requires extracting more data to represent the DHT design configuration that exists in the plant. However, different data extraction options can be considered to represent the DHT configuration depending on the associated process/operation constraints. This work describes a systematic procedure to extract and analyse the impacts of DHT on the overall energy efficiency of a Kraft process with a specific focus on mixing along the pulp line and in water tanks

  18. Direct numerical simulation of circular-cap bubbles in low viscous liquids using counter diffusion lattice Boltzmann method

    Energy Technology Data Exchange (ETDEWEB)

    Ryu, Seungyeob, E-mail: syryu@kaeri.re.kr [Korea Atomic Energy Research Institute (KAERI), 1045 Daeduk-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Kim, Youngin; Yoon, Juhyeon [Korea Atomic Energy Research Institute (KAERI), 1045 Daeduk-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Ko, Sungho, E-mail: sunghoko@cnu.ac.kr [Department of Mechanical Design Engineering, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764 (Korea, Republic of)

    2014-01-15

    Highlights: • We directly simulate circular-cap bubbles in low viscous liquids. • The counter diffusion multiphase lattice Boltzmann method is proposed. • The present method is validated through benchmark tests and experimental results. • The high-Reynolds-number bubbles can be simulated without any turbulence models. • The present method is feasible for the direct simulation of bubbly flows. -- Abstract: The counter diffusion lattice Boltzmann method (LBM) is used to directly simulate rising circular-cap bubbles in low viscous liquids. A counter diffusion model for single phase flows has been extended to multiphase flows, and the implicit formulation is converted into an explicit one for easy calculation. Bubbles at high Reynolds numbers ranging from O(10{sup 2}) to O(10{sup 4}) are simulated successfully without any turbulence models, which cannot be done for the existing LBM versions. The characteristics of the circular-cap bubbles are studied for a wide range of Morton numbers and compared with the previous literature. Calculated results agree with the theoretical and experimental data. Consequently, the wake phenomena of circular-cap bubbles and bubble induced turbulence are presented.

  19. Application of multi-step direct reaction theory to 14 MeV neutron reaction, 3 (n,. cap alpha. )

    Energy Technology Data Exchange (ETDEWEB)

    Kumabe, I.; Matoba, M.; Fukuda, K. [Kyushu Univ., Fukuoka (Japan). Faculty of Engineering; Ikegami, H.; Muraoka, M [eds.

    1980-01-01

    Multi-step direct-reaction theory proposed by Tamura et al. has been applied to continuous spectra of the 14 MeV (n, ..cap alpha..) reaction with some modifications. Calculated results reproduce well the experimental energy and angular distributions of the 14 MeV (n, ..cap alpha..) reactions.

  20. Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration.

    Science.gov (United States)

    Hu, Lei; Gao, Zhenhua; Xu, Junji; Zhu, Zhao; Fan, Zhipeng; Zhang, Chunmei; Wang, Jinsong; Wang, Songlin

    2017-01-01

    Endodontic regeneration shows promise in treating dental pulp diseases; however, no suitable scaffolds exist for pulp regeneration. Acellular natural extracellular matrix (ECM) is a favorable scaffold for tissue regeneration since the anatomical structure and ECM of the natural tissues or organs are well-preserved. Xenogeneic ECM is superior to autologous or allogeneic ECM in tissue engineering for its unlimited resources. This study investigated the characteristics of decellularized dental pulp ECM from swine and evaluated whether it could mediate pulp regeneration. Dental pulps were acquired from the mandible anterior teeth of swine 12 months of age and decellularized with 10% sodium dodecyl sulfate (SDS) combined with Triton X-100. Pulp regeneration was conducted by seeding human dental pulp stem cells into decellularized pulp and transplanted subcutaneously into nude mice for 8 weeks. The decellularized pulp demonstrated preserved natural shape and structure without any cellular components. Histological analysis showed excellent ECM preservation and pulp-like tissue, and newly formed mineralized tissues were regenerated after being transplanted in vivo. In conclusion, decellularized swine dental pulp maintains ECM components favoring stem cell proliferation and differentiation, thus representing a suitable scaffold for improving clinical outcomes and functions of teeth with dental pulp diseases.

  1. Path analysis for selection of feijoa with greater pulp weight

    Directory of Open Access Journals (Sweden)

    Joel Donazzolo

    Full Text Available ABSTRACT: The objective of this paper was to identify the direct and indirect effects of feijoa fruits (Acca sellowiana traitson pulp weight, in order to use these traits in indirect genotypes selection. Fruits of five feijoa plants were collected in Rio Grande do Sul, in the years of 2009, 2010 and 2011. Six traits were evaluated: diameter, length, total weight, pulp weight, peel thickness and number of seeds per fruit. In the path analysis, with or without ridge regression, pulp weight was considered as the basic variable, and the other traits were considered as explanatory variables. Total weight and fruit diameter had high direct effect, and are the main traits associated with pulp weight. These traits may serve as criteria for indirect selection to increase feijoa pulp weight, since they are easy to be measured.

  2. Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration

    Directory of Open Access Journals (Sweden)

    Lei Hu

    2017-01-01

    Full Text Available Endodontic regeneration shows promise in treating dental pulp diseases; however, no suitable scaffolds exist for pulp regeneration. Acellular natural extracellular matrix (ECM is a favorable scaffold for tissue regeneration since the anatomical structure and ECM of the natural tissues or organs are well-preserved. Xenogeneic ECM is superior to autologous or allogeneic ECM in tissue engineering for its unlimited resources. This study investigated the characteristics of decellularized dental pulp ECM from swine and evaluated whether it could mediate pulp regeneration. Dental pulps were acquired from the mandible anterior teeth of swine 12 months of age and decellularized with 10% sodium dodecyl sulfate (SDS combined with Triton X-100. Pulp regeneration was conducted by seeding human dental pulp stem cells into decellularized pulp and transplanted subcutaneously into nude mice for 8 weeks. The decellularized pulp demonstrated preserved natural shape and structure without any cellular components. Histological analysis showed excellent ECM preservation and pulp-like tissue, and newly formed mineralized tissues were regenerated after being transplanted in vivo. In conclusion, decellularized swine dental pulp maintains ECM components favoring stem cell proliferation and differentiation, thus representing a suitable scaffold for improving clinical outcomes and functions of teeth with dental pulp diseases.

  3. Direct heat transfer considerations for improving energy efficiency in pulp and paper Kraft mills

    Energy Technology Data Exchange (ETDEWEB)

    Savulescu, Luciana Elena; Alva-Argaez, Alberto [Natural Resources (Canada)

    2008-10-15

    The success of any process improvement study depends on the quality of the available data and the way in which the plant-specific characteristics are incorporated in the applied conceptual models; in the context of process integration studies these issues are directly related to the rules followed during the data extraction stage. Improving energy efficiency in a pulp and paper Kraft mill requires the identification of the most promising heat recovery network retrofit projects. In a retrofit analysis using pinch technology/process integration methods, only the process streams associated to the existing heat exchangers and some outlet streams (such as wastewater/effluent streams and vents) with high potential for heat recovery are usually included, while the energy exchanged through non-isothermal stream mixing (NIM) or direct heat transfer (DHT) is often assumed fixed and is not considered in the analysis. Relaxing this assumption requires extracting more data to represent the DHT design configuration that exists in the plant. However, different data extraction options can be considered to represent the DHT configuration depending on the associated process/operation constraints. This work describes a systematic procedure to extract and analyse the impacts of DHT on the overall energy efficiency of a Kraft process with a specific focus on mixing along the pulp line and in water tanks. (author)

  4. Method for rapidly determining a pulp kappa number using spectrophotometry

    Science.gov (United States)

    Chai, Xin-Sheng; Zhu, Jun Yong

    2002-01-01

    A system and method for rapidly determining the pulp kappa number through direct measurement of the potassium permanganate concentration in a pulp-permanganate solution using spectrophotometry. Specifically, the present invention uses strong acidification to carry out the pulp-permanganate oxidation reaction in the pulp-permanganate solution to prevent the precipitation of manganese dioxide (MnO.sub.2). Consequently, spectral interference from the precipitated MnO.sub.2 is eliminated and the oxidation reaction becomes dominant. The spectral intensity of the oxidation reaction is then analyzed to determine the pulp kappa number.

  5. Pulp stem cells: implication in reparative dentin formation.

    Science.gov (United States)

    Dimitrova-Nakov, Sasha; Baudry, Anne; Harichane, Yassine; Kellermann, Odile; Goldberg, Michel

    2014-04-01

    Many dental pulp stem cells are neural crest derivatives essential for lifelong maintenance of tooth functions and homeostasis as well as tooth repair. These cells may be directly implicated in the healing process or indirectly involved in cell-to-cell diffusion of paracrine messages to resident (pulpoblasts) or nonresident cells (migrating mesenchymal cells). The identity of the pulp progenitors and the mechanisms sustaining their regenerative capacity remain largely unknown. Taking advantage of the A4 cell line, a multipotent stem cell derived from the molar pulp of mouse embryo, we investigated the capacity of these pulp-derived precursors to induce in vivo the formation of a reparative dentin-like structure upon implantation within the pulp of a rodent incisor or a first maxillary molar after surgical exposure. One month after the pulp injury alone, a nonmineralized fibrous matrix filled the mesial part of the coronal pulp chamber. Upon A4 cell implantation, a mineralized osteodentin was formed in the implantation site without affecting the structure and vitality of the residual pulp in the central and distal parts of the pulp chamber. These results show that dental pulp stem cells can induce the formation of reparative dentin and therefore constitute a useful tool for pulp therapies. Finally, reparative dentin was also built up when A4 progenitors were performed by alginate beads, suggesting that alginate is a suitable carrier for cell implantation in teeth. Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  6. EZH2 regulates dental pulp inflammation by direct effect on inflammatory factors.

    Science.gov (United States)

    Hui, Tianqian; A, Peng; Zhao, Yuan; Yang, Jing; Ye, Ling; Wang, Chenglin

    2018-01-01

    Pulpitis is a multi-factorial disease that could be caused by complex interactions between genetics, epigenetics and environmental factors. We aimed to evaluate the role of Enhancer of Zeste Homolog 2 (EZH2) in the inflammatory response of human dental pulp cells (HDPCs) and dental pulp tissues. The expressions of inflammatory cytokines in HDPCs treated by EZH2 complex or EZH2 siRNA with or without rhTNF-α were examined by quantitative real-time polymerase chain reaction (q-PCR). The levels of secreted inflammatory cytokines including IL-6, IL-8, IL-15, CCL2 and CXCL12 in culture supernatants were measured by Luminex assay. In rat pulpitis model, the effects of EZH2 on dental pulp tissues were verified by histology. We invested the mechanisms of the effect of EZH2 on the inflammatory factors by ChIP assay. EZH2 down-regulation inhibited the expression of inflammatory factors, including IL-6, IL-8, IL-15, CCL2 and CXCL12 in HDPCs. EZH2 complex promoted the expression and secretion of these inflammatory factors in HDPCs, while EZH2 silencing could attenuate the promotion of inflammatory factors that were induced by rhTNF-α. In pulpitis models of rats, EZH2 down-regulation inhibited the inflammatory process of dental pulp while EZH2 complex showed no significant facilitation of pulpal inflammation. In addition, EZH2 could bind on the promoters of IL-6, IL-8 and CCL2, but not IL-15 and CXCL12, to affect the transcription of these proinflammatory cytokines. In HDPCs, EZH2 could induce inflammation, while EZH2 down-regulation could attenuate the inflammatory responses. EZH2 plays an important role in this inflammatory process of dental pulp. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Microscopic analysis of dog dental pulp after pulpotomy and pulp protection with mineral trioxide aggregate and white Portland cement Análise microscópica da polpa dental de cães após pulpotomia e proteção pulpar com agregado de trióxido mineral e cimento Portland branco

    Directory of Open Access Journals (Sweden)

    Renato Menezes

    2004-06-01

    Full Text Available Considering previous studies on the similarity between the chemical composition of the mineral trioxide aggregate and the Portland cement, the purpose of this study was to investigate the pulp response of dog's teeth after pulpotomy and direct pulp protection with MTA Angelus and white Portland cement. Thirty eight pulp remnants were protected with these materials. One hundred and twenty days after treatment, the animals were sacrificed and the specimens removed and prepared for histological analysis. Both materials demonstrated the same results when used as pulp capping materials, inducing hard tissue bridge formation and maintaining pulp vitality in all specimens. The MTA Angelus and the white Portland cement showed to be effective as pulp protection materials following pulpotomy.Considerando estudos anteriores sobre a similaridade entre a composição química do agregado de trióxido mineral e o cimento Portland, o objetivo deste estudo foi investigar a resposta pulpar de dentes de cães após pulpotomia e proteção pulpar direta com MTA Angelus e cimento Portland branco. Trinta e oito remanescentes pulpares foram recobertos com esses materiais. Cento e vinte dias após o tratamento, os animais foram sacrificados e os espécimes removidos e preparados para análise histológica. Ambos os materiais demonstraram os mesmos resultados quando utilizados como materiais de capeamento pulpar, induzindo a formação de ponte de tecido mineralizado e mantendo a vitalidade pulpar em todos os espécimes. Ambos matérias se mostraram efetivos como protetores pulpares após pulpotomia em dentes de cães.

  8. Method and apparatus for assaying wood pulp fibers

    Science.gov (United States)

    Gustafson, Richard [Bellevue, WA; Callis, James B [Seattle, WA; Mathews, Jeffrey D [Neenah, WI; Robinson, John [Issaquah, WA; Bruckner, Carsten A [San Mateo, CA; Suvamakich, Kuntinee [Seattle, WA

    2009-05-26

    Paper pulp is added to a stain solution. The stain solution and pulp fibers are mixed to form a slurry. Samples are removed from the slurry and are admixed with dilution water and a bleach. Then, the fibers are moved into a flow cell where they are subjected to a light source adapted to stimulate fluorescence from the stained pulp fiber. Before the fiber slurry enters the flow cell it is mixed with a dilution water of bleach to reduce background fluorescence. The fluorescent light is collimated and directed through a dichroic filter onto a fluorescence splitting dichroic filter.

  9. The resin-in-pulp process and its application to ores from Brosses ''BRS 10''; Le procede resin in pulp et son application aux minerais des Brosses ''BRS 10''

    Energy Technology Data Exchange (ETDEWEB)

    Kremer, M

    1959-03-01

    The resin-in-pulp process is a technical variant of the recovery process of uranium in dilute solution by means of ion exchanger resins. An anion resin, XE 123, of a well-defined grain size is placed in direct contact with the pulp produced by sulfuric acid attack on ore with a low uranium content. This process is of particular value in the treatment of pulps that cannot be filtered or decanted, such as those obtained with ore from Brosses. The preparation of the pulp, the elution of the uranium, and its fixation, as well as the various factors encountered in these operations, are discussed. (author) [French] Le procede ''resin in pulp'' est une variante technique du procede de recuperation de l'uranium en solution diluee par les resines echangeuses d'ions. Une resine anionique, la 'XE 123' a granulometrie bien determinee, est mise en contact direct avec la pulpe provenant de l'attaque a l'acide sulfurique d'un minerai d'uranium a faible teneur. Ce procede est particulierement interessant dans le cas de pulpes infiltrables ou indecantables, telles que celles obtenues dans l'attaque du minerai des Brosses. La preparation de la pulpe, la fixation et l'elution de l'uranium, ainsi que les facteurs intervenant dans ces diverses operations sont etudies dans le present rapport. (auteur)

  10. High-alkali low-temperature polysulfide pulping (HALT) of Scots pine.

    Science.gov (United States)

    Paananen, Markus; Sixta, Herbert

    2015-10-01

    High-alkali low-temperature polysulfide pulping (HALT) was effectively utilised to prevent major polysaccharide losses while maintaining the delignification rate. A yield increase of 6.7 wt% on wood was observed for a HALT pulp compared to a conventionally produced kappa number 60 pulp with comparable viscosity. Approximately 70% of the yield increase was attributed to improved galactoglucomannan preservation and 30% to cellulose. A two-stage oxygen delignification sequence with inter-stage peroxymonosulphuric acid treatment was used to ensure delignification to a bleachable grade. In a comparison to conventional pulp, HALT pulp effectively maintained its yield advantage. Diafiltration trials indicate that purified black liquor can be directly recycled, as large lignin fractions and basically all dissolved polysaccharides were separated from the alkali-rich BL. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Wheat Bread with Pumpkin (Cucurbita maxima L. Pulp as a Functional Food Product

    Directory of Open Access Journals (Sweden)

    Renata Różyło

    2014-01-01

    Full Text Available In this study, a new application of pumpkin pulp in bread production is shown. The aim of this work is to determine the influence of the addition of fresh pumpkin pulp directly into wheat flour on physical, sensorial and biological properties of bread. The bioaccessibility of active compounds was also studied. An increase in the addition of pumpkin pulp from 5 to 20 % (converted to dry matter caused a decrease of bread volume and increase of crumb hardness and cohesiveness. The sensory characteristics of the bread showed that a partial replacement of wheat fl our with up to 10 % of pumpkin pulp gave satisfactory results. The taste, aroma and overall acceptability of control bread and bread containing 5 or 10 % of pulp had the highest degree of liking. The addition of higher levels of pumpkin pulp caused an unpleasant aroma and taste. Pumpkin pulp is a good material to complement the bread with potentially bioaccessible phenolics (including flavonoids and, especially, with peptides. The highest antioxidant activity was observed, in most cases, of the samples with added 10 and 15 % of pumpkin pulp. The addition of the pulp significantly enriched the bread with potentially bioaccessible angiotensin-converting enzyme (ACE inhibitors. The highest activity was determined in the bread with 15 and 20 % pumpkin pulp. ACE inhibitors from the tested bread were highly bioaccessible in vitro. Pumpkin pulp seems to be a valuable source of active compounds to complement the wheat bread. Adding the pulp directly to the wheat flour gives satisfactory baking results and reduces the cost of production. Additionally, pumpkin pulp is sometimes treated as waste material after the acquisition of seeds, thus using it as bread supplement also has environmental and economic benefi ts.

  12. Vital Pulp Therapy—Current Progress of Dental Pulp Regeneration and Revascularization

    Directory of Open Access Journals (Sweden)

    Weibo Zhang

    2010-01-01

    Full Text Available Pulp vitality is extremely important for the tooth viability, since it provides nutrition and acts as biosensor to detect pathogenic stimuli. In the dental clinic, most dental pulp infections are irreversible due to its anatomical position and organization. It is difficult for the body to eliminate the infection, which subsequently persists and worsens. The widely used strategy currently in the clinic is to partly or fully remove the contaminated pulp tissue, and fill and seal the void space with synthetic material. Over time, the pulpless tooth, now lacking proper blood supply and nervous system, becomes more vulnerable to injury. Recently, potential for successful pulp regeneration and revascularization therapies is increasing due to accumulated knowledge of stem cells, especially dental pulp stem cells. This paper will review current progress and feasible strategies for dental pulp regeneration and revascularization.

  13. Effect of radiation on the response of dental pulp to operative and endodontic procedures: an experimental study

    International Nuclear Information System (INIS)

    Fawzi, M.I.; Shklar, G.; Krakow, A.A.

    1985-01-01

    Thirty-six 56-day-old male Sprague-Dawley albino rats served as two groups of experimental animals. Group 1 was irradiated with 400 rads delivered as total-body radiation from a cesium source. Group 2 served as the control group and was not irradiated. Three weeks later, the dental microscope was used to facilitate various dental procedures in both groups of animals (cavity preparation filled with zinc oxide-eugenol, pulp exposure capped with zinc oxide-eugenol, and pulp exposure left open). Two animals for each procedure from Groups 1 and 2 were killed at time intervals of 2, 4, and 8 weeks. The results showed that (1) radiation at this dose resulted in a depression of the normal response of the dental pulp to the trauma and infection induced by pulpal exposure, (2) there were no pathologic changes in the untreated molars of the irradiated animals, and (3) the use of the dental microscope greatly facilitated cavity preparation in the molars of rats

  14. Claustral single cell reactions to tooth pulp stimulation in cats.

    Science.gov (United States)

    Jastreboff, P; Sikora, M; Frydrychowski, A; Słoniewski, P

    1983-01-01

    Single unit activity in the central region of the claustrum, evoked by electrical stimulation of tooth pulp or paws was studied on cats under chloralose anesthesia. The majority of cells responded in similar manner to stimulation of tooth pulp or paws, but there were cells with clear preference to a given type of stimulation. Latencies of reactions evoked by tooth pulp stimulation were significantly shorter than those for limb stimulation. In the former case latencies as short as 8 rns were observed. It is postulated that the central region of the claustrum receives a projection from the tooth pulp, and that in those cases with very short latency the projection is direct and does not involve the cerebral cortex.

  15. Organic-Inorganic Nanostructure Architecture via Directly Capping Fullerenes onto Quantum Dots.

    Science.gov (United States)

    Lee, Jae Kwan; Kim, Jonggi; Yang, Changduk

    2011-12-01

    A new form of fullerene-capped CdSe nanoparticles (PCBA-capped CdSe NPs), using carboxylate ligands with [60]fullerene capping groups that provides an effective synthetic methodology to attach fullerenes noncovalently to CdSe, is presented for usage in nanotechnology and photoelectric fields. Interestingly, either the internal charge transfer or the energy transfer in the hybrid material contributes to photoluminescence (PL) quenching of the CdSe moieties.

  16. Ground penetrating radar and direct current resistivity evaluation of the desiccation test cap, Savannah River Site

    International Nuclear Information System (INIS)

    Wyatt, D.E.; Cumbest, R.J.

    1996-04-01

    The Savannah River Site (SRS) has a variety of waste units that may be temporarily or permanently stabilized by closure using an impermeable cover to prevent groundwater infiltration. The placement of an engineered kaolin clay layer over a waste unit is an accepted and economical technique for providing an impermeable cover but the long term stability and integrity of the clay in non-arid conditions is unknown. A simulated kaolin cap has been constructed at the SRA adjacent to the Burial Ground Complex. The cap is designed to evaluate the effects of desiccation on clay integrity, therefore half of the cap is covered with native soil to prevent drying, while the remainder of the cap is exposed. Measurements of the continuing impermeability of a clay cap are difficult because intrusive techniques may locally compromise the structure. Point measurements made to evaluate clay integrity, such as those from grid sampling or coring and made through a soil cover, may miss cracks, joints or fissures, and may not allow for mapping of the lateral extent of elongate features. Because of these problems, a non-invasive technique is needed to map clay integrity, below a soil or vegetation cover, which is capable of moderate to rapid investigation speeds. Two non-intrusive geophysical techniques, direct current resistivity and ground penetrating radar (GPR), have been successful at the SRS in geologically mapping shallow subsurface clay layers. The applicability of each technique in detecting the clay layer in the desiccation test cap and associated anomalies was investigated

  17. Organic–Inorganic Nanostructure Architecture via Directly Capping Fullerenes onto Quantum Dots

    Directory of Open Access Journals (Sweden)

    Kim Jonggi

    2011-01-01

    Full Text Available Abstract A new form of fullerene-capped CdSe nanoparticles (PCBA-capped CdSe NPs, using carboxylate ligands with [60]fullerene capping groups that provides an effective synthetic methodology to attach fullerenes noncovalently to CdSe, is presented for usage in nanotechnology and photoelectric fields. Interestingly, either the internal charge transfer or the energy transfer in the hybrid material contributes to photoluminescence (PL quenching of the CdSe moieties.

  18. Effect of organosolv and soda pulping processes on the metals content of non-woody pulps.

    Science.gov (United States)

    González, M; Cantón, L; Rodríguez, A; Labidi, J

    2008-09-01

    In this work the effect of different pulping processes (ethyleneglycol, diethyleneglycol, ethanolamine and soda) of tow abounded raw materials (empty fruit bunches - EFB and rice straw) on the ash, silicates and metals (Fe, Zn, Cu, Pb, Mn, Ni and Cd) content of the obtained pulps have been studied. Results showed that pulps obtained by diethyleneglycol pulping process presented lower metals content (756 microg/g and 501 microg/g for EFB and rice straw pulp, respectively) than soda pulps (984 microg/g and 889 microg/g). Ethanolamine pulps presented values of holocellulose (74% and 77% for EFB and rice straw pulp, respectively), alpha-cellulose (74% and 69%), kappa number (18.7 and 18.5) and viscosity (612 and 90 6ml/g) similar to those of soda pulp, and lower lignin contents (11% and 12%).

  19. Evaluation of a value prior to pulping-thermomechanical pulp business concept. Part 2.

    Science.gov (United States)

    Ted Bilek; Carl Houtman; Peter Ince

    2011-01-01

    Value Prior to Pulping (VPP) is a novel biorefining concept for pulp mills that includes hydrolysis extraction of hemicellulose wood sugars and acetic acid from pulpwood prior to pulping. The concept involves conversion of wood sugars via fermentation to fuel ethanol or other chemicals and the use of remaining solid wood material in the pulping process. This paper...

  20. Histologic evaluation of pulpal response to MTA and capsaicin in cats

    Directory of Open Access Journals (Sweden)

    Zahrasadat Madani

    2012-09-01

    Full Text Available Introduction: In direct pup capping, the exposed pulp is directly capped with a capping material to provoke a dentinal bridge formation to seal the exposed area.The aim of this study was to evaluate the pulpal response to MTA and capsaicin in cats. Methods: The sample for this experimental study consisted of 24 canines of cats, weighed approximately 3-4kg. After sedation, the teeth were cut and exposed at 3mm above cementoenamel junction, then divided into two groups: 1 direct pulp capping with MTA 2 direct pulp capping with Capsaicin. The cavities were filled with glass ionomer cement (Fuji IX. The cats were sacrificed in the first, second, and fourth weeks 4 canine teeth were extracted for the purpose of histologic analysis. 6Serial sections were cut parallel to the longitudinal axis of the canines. The sections were stained with hematoxylin & eosin, and were observed and analyzed using a light microscope. Results: In both groups treated with MTA and capsaicin, the inflammation decreased during weeks1, 2, and 4. Less inflammation was seen in MTA group but there was no statistical difference between the two groups (p=0.22. In the group treated with capsaicin, necrosis was observed in every 12 samples, but in the other group treated with MTA, only 1 pulp necrosis was seen in the first week (p=0.000. There was no significant difference in dentinal barrier formation, inflammatory response, and soft tissue changes between the two groups. Conclusions: The results of this study showed that capsaicin can decrease the severity of inflammation, but it is ineffective in dentinal barrier formation.

  1. Recruitment of dental pulp cells by dentine and pulp extracellular matrix components.

    Science.gov (United States)

    Smith, J G; Smith, A J; Shelton, R M; Cooper, P R

    2012-11-01

    The present study aimed to determine whether dentine tissue and preparations of extracellular matrix (ECM) from pulp (pECM) and dentine (dECM), and breakdown products, influenced pulp cell migration. Chemotaxis transwell and agarose spot assays demonstrated that both dentine and pulp ECM molecules acted as chemoattractants for primary pulp cells. Chemoattractant activities of dECM and pECM were enhanced when subjected to acid and enzymatic breakdown, respectively. This enhanced activity following physiologically relevant breakdown may be pertinent to the disease environment. Pulp cell migration in response to dental ECMs was dependent on an active rho pathway. Recruited cells exhibited increased stem cell marker expression indicating that dental ECMs and their breakdown products selectively attract progenitor cells that contribute to repair processes. In conclusion, combined these results indicate that ECM molecules contribute to cell recruitment necessary for regeneration of the dentine-pulp complex after injury. Copyright © 2012 Elsevier Inc. All rights reserved.

  2. Radiation -adsorption treatment of pesticides by using wood pulp and bagasse pulp

    International Nuclear Information System (INIS)

    Abd El-Aal, S.E.; Sokker, S.S.; Dessooki, A.M.

    2005-01-01

    Alkaline pulping of pulp wood and bagasse using sodium hydroxide resulted in the reduction of lignin from the wood and bagasse fibers and consequently increase adsorption of the pesticide pollutants to these fibers. Three different types of pesticides were used in this study namely, metalaxyl, dicloran and arelon. which were irradiated at a dose of 4 kGy before adsorption treatment.The results show that moderate adsorption was observed for all pesticides when adsorption was carried out without alkaline pulping and irradiation. This is due to the presence of lignin which retard the adsorption process. Batch sorption experiments at different pH values (3, 7, 9) for the retention of these pesticides by pulp wood and pulp bagasse fibers indicated that sorption is governed by the interaction of the ionized form of these compounds with the polyhydroxyl structure of cellulose. The study shows that alkaline pulping of pulpwood and bagasse improves its ability towards adsorption of the radiation degraded pesticide molecules

  3. Genetic ablation of root cap cells in Arabidopsis

    OpenAIRE

    Tsugeki, Ryuji; Fedoroff, Nina V.

    1999-01-01

    The root cap is increasingly appreciated as a complex and dynamic plant organ. Root caps sense and transmit environmental signals, synthesize and secrete small molecules and macromolecules, and in some species shed metabolically active cells. However, it is not known whether root caps are essential for normal shoot and root development. We report the identification of a root cap-specific promoter and describe its use to genetically ablate root caps by directing root cap-specific expression of...

  4. The Relix process for the resin-in-pulp recovery of uranium

    International Nuclear Information System (INIS)

    Cloete, F.L.D.

    1981-01-01

    The Relix process is based on direct contact between an ion-exchange resin and undiluted pulp, thus avoiding prior solid-liquid separation. The resin particles float near the surface of the pulp, forming an inverted fluidized bed with the pulp flowing downwards. The basic idea was demonstrated on a full-scale pachuca tank at Stilfontein Gold Mine in 1970, followed by a small-scale demonstration run in a laboratory at the National Institute for Metallurgy. A pilot plant based on a throughput of 60 tons of ore per day was subsequently operated at West Driefontein Gold Mine for several periods over two years. Although the plant proved operable from a mechanical point of view, the metallurgical performance was not up to expectation. The basic cause of the poor metallurgical performance was shown to be backmixing of both the resin and the pulp between stages. The values obtained for resin losses were inconclusive. Further development of resin-in-pulp processes for the recovery of uranium should be focused on the performance of various techniques for the screening of resin from pulp [af

  5. Direct observation of strain in InAs quantum dots and cap layer during molecular beam epitaxial growth using in situ X-ray diffraction

    Energy Technology Data Exchange (ETDEWEB)

    Shimomura, Kenichi; Ohshita, Yoshio; Kamiya, Itaru, E-mail: kamiya@toyota-ti.ac.jp [Toyota Technological Institute, 2-12-1 Hisakata, Tempaku, Nagoya 468-8511 (Japan); Suzuki, Hidetoshi [Faculty of Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192 (Japan); Sasaki, Takuo; Takahasi, Masamitu [Quantum Beam Science Center, Japan Atomic Energy Agency, Koto 1-1-1, Sayo-cho, Hyogo 679-5148 (Japan)

    2015-11-14

    Direct measurements on the growth of InAs quantum dots (QDs) and various cap layers during molecular beam epitaxy are performed by in situ X-ray diffraction (XRD). The evolution of strain induced both in the QDs and cap layers during capping is discussed based on the XRD intensity transients obtained at various lattice constants. Transients with different features are observed from those obtained during InGaAs and GaAs capping. The difference observed is attributed to In-Ga intermixing between the QDs and the cap layer under limited supply of In. Photoluminescence (PL) wavelength can be tuned by controlling the intermixing, which affects both the strain induced in the QDs and the barrier heights. The PL wavelength also varies with the cap layer thickness. A large redshift occurs by reducing the cap thickness. The in situ XRD observation reveals that this is a result of reduced strain. We demonstrate how such information about strain can be applied for designing and preparing novel device structures.

  6. Direct observation of strain in InAs quantum dots and cap layer during molecular beam epitaxial growth using in situ X-ray diffraction

    International Nuclear Information System (INIS)

    Shimomura, Kenichi; Ohshita, Yoshio; Kamiya, Itaru; Suzuki, Hidetoshi; Sasaki, Takuo; Takahasi, Masamitu

    2015-01-01

    Direct measurements on the growth of InAs quantum dots (QDs) and various cap layers during molecular beam epitaxy are performed by in situ X-ray diffraction (XRD). The evolution of strain induced both in the QDs and cap layers during capping is discussed based on the XRD intensity transients obtained at various lattice constants. Transients with different features are observed from those obtained during InGaAs and GaAs capping. The difference observed is attributed to In-Ga intermixing between the QDs and the cap layer under limited supply of In. Photoluminescence (PL) wavelength can be tuned by controlling the intermixing, which affects both the strain induced in the QDs and the barrier heights. The PL wavelength also varies with the cap layer thickness. A large redshift occurs by reducing the cap thickness. The in situ XRD observation reveals that this is a result of reduced strain. We demonstrate how such information about strain can be applied for designing and preparing novel device structures

  7. EVALUATION OF LINERBOARD PROPERTIES FROM MALAYSIAN CULTIVATED KENAF SODA-ANTHRAQUINONE PULPS VERSUS COMMERCIAL PULPS

    Directory of Open Access Journals (Sweden)

    Ahmad Azizi Mossello

    2010-06-01

    Full Text Available Malaysian cultivated kenaf has been identified as a suitable raw material for linerboard production. This study examines the soda-antraquinone (soda-AQ pulp of kenaf fibers versus old corrugated container (OCC and unbleached softwood kraft pulps as the main sources for linerboard production. The results showed significant differences among the pulp properties. The unbleached kraft pulp with very high freeness required high beating to reach an optimized freeness and produced paper with the highest strength properties, except for tear resistance. The OCC gave paper with the lowest strength properties. In the case of kenaf fractions, bast pulp with high freeness needed less beating than softwood and produced paper with high tear resistance. Core fiber, which had the lowest freeness and highest drainage time, led to paper with high strength but very low tear resistance. Kenaf whole stem pulp showed intermediate properties between core and bast and close to those of unbleached softwood pulp, but with very lower beating requirement. Finally, kenaf whole stem, due to its strength properties, moderate separation cost, and simple pulping process, was judged to be more suitable for commercialization for linerboard production in Malaysia.

  8. Effects of Soda-Anthraquinone Pulping Variables on the Durian Rind Pulp and Paper Characteristics: A Preliminary Test

    Science.gov (United States)

    Rizal Masrol, Shaiful; Irwan Ibrahim, Mohd Halim; Adnan, Sharmiza; Rahmad Talib, Mohd; Sian, Lau Lee

    2017-08-01

    Good combination of pulping variables is required to obtain the quality pulp and paper characteristics. Thus, in this preliminary work, naturally dried durian rind were pulped under Soda-Anthraquinone (Soda-AQ) pulping process with 18% to 22% alkali charge, 0% to 0.1% Anthraquinone (AQ) charge, 90 minutes to 150 minutes of cooking time and 150°C to 170°C to investigate the effect of pulping variables on the characteristics of the pulp and paper. Pulping condition with 0% of AQ charge was also conducted for comparison. Results indicated that the best screen yield percentage, reject yield percentage, freeness, drainage time, tear index, number of folds and optical properties were shown by the pulp produced with combination of the highest active alkali (22%), AQ charge (0.1%), cooking time (150 minutes) and cooking temperature (170°C) except apparent density, tensile index and burst index. This preliminary result shows that the optimum quality of durian rind pulp as a potential papermaking raw material pulp could be produced by selecting the good combination of pulping variables which influences the pulp and paper characteristics.

  9. The application of lesion sterilization and tissue repair 3Mix-MP for treating rat's dental pulp tissue

    Directory of Open Access Journals (Sweden)

    Raditya Nugroho

    2015-03-01

    Full Text Available Background: Lesion sterilization and tissue repair (LSTR 3Mix-MP are three broad-spectrum antibiotics, including metronidazole, ciprofloxacin and minocycline are mixed with propylene glycol or macrogol. There is the possibility ofthe healing process that marked proliferation ofnew blood vessels and proliferation offibroblasts in the treatment ofirreversible pulpitis by pulp capping LSTR 3MixMP because of  the principle of the method LSTR 3Mix-MP is to kill bacteria. Purpose: The purpose of this study to prove the effect of LSTR 3Mix-MP on chronic inflammation and the healing process in rat dental pulp tissue in vivo. Methods: Rattus norvegicus anaesthetized by using ketamine and xylazine dissolved in sterile isotonic saline solution (0.2 ml/50gr mm on the upper right thigh. Cavity preparation class I to perforation by using a low speed tapered diamond round bur. In the treatment group, rats were treated 3Mix-MP at a dose of10 mg and then covered with glass ionomer cement for 7 days on the pulp that has been opened for 3 days. The control group treated with saline irrigation on the pulp that has been opened for 3 days. Rats were killed after seven days, and then made preparations pulp tissue to count the number oflymphocytes, macrophages, plasma cells, blood vessels, and fibroblasts Results: There is an increase in the average number ofmacrophage cells, plasma, and fibroblasts; and decreased lymphocytes and blood vessels in the treated group exposure LSTR 3Mix-MP. Conclusion:LSTR 3Mix-MP can reduce chronic inflammation process and enhance the healing process in rat dental pulp tissue.

  10. Characterization of Coronal Pulp Cells and Radicular Pulp Cells in Human Teeth.

    Science.gov (United States)

    Honda, Masaki; Sato, Momoko; Toriumi, Taku

    2017-09-01

    Dental pulp has garnered much attention as an easily accessible postnatal tissue source of high-quality mesenchymal stem cells (MSCs). Since the discovery of dental pulp stem cells (DPSCs) in permanent third molars, stem cells from human exfoliated deciduous teeth and from supernumerary teeth (mesiodentes) have been identified as a population distinct from DPSCs. Dental pulp is divided into 2 parts based on the developing stage: the coronal pulp and the radicular pulp. Root formation begins after the crown part is completed. We performed a sequential study to examine the differences between the characteristics of coronal pulp cells (CPCs) and radicular pulp cells (RPCs) from permanent teeth, mesiodentes, and deciduous teeth. Interestingly, although we have not obtained any data on the difference between CPCs and RPCs in permanent teeth, there are some differences between the characteristics of CPCs and RPCs from mesiodentes and deciduous teeth. The MSC characteristics differed between the RPCs and CPCs, and the reprogramming efficiency for the generation of induced pluripotent stem cells was greater in RPCs than in CPCs from deciduous teeth. The proportion of CD105 + cells in CPCs versus that in RPCs varied in mesiodentes but not in permanent teeth. The results indicate that the proportion of CD105 + cells is an effective means of characterizing dental pulp cells in mesiodentes. Taken together, the stem cells in deciduous and supernumerary teeth share many characteristics, such as a high proliferation rate and an immunophenotype similar to that of DPSCs. Thus, mesiodentes accidentally encountered on radiographs by the general dental practitioner might be useful for stem cell therapy. Copyright © 2017 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  11. Effect of radiopaque Portland cement on mineralization in human dental pulp cells.

    Science.gov (United States)

    Min, Kyung-San; Lee, Sang-Im; Lee, Yoon; Kim, Eun-Cheol

    2009-10-01

    The aim of this study was to investigate whether radiopaque Portland cement (RPC) facilitates the mineralization process in human dental pulp cells (HDPCs) compared with pure Portland cement (PC). Under a scanning electron microscope (SEM), cellular morphology was evaluated. Alkaline phosphatase (ALP) activity was analyzed, and nodule formation was assessed by performing Alizarin Red S staining. In addition, the mRNA expressions of mineralization-related proteins were evaluated by performing a real-time polymerase chain reaction. On SEM evaluation, healthy HDPCs were found adhering to the surfaces of PC and RPC. The ALP activity increased in the PC and RPC groups compared with the control group at 1 day. Alizarin Red stain increased in the PC and RPC groups compared with the control group at 2 and 3 weeks. The mRNA expression of dentin sialophosphoprotein increased at 14 days in the PC and RPC groups. These results show that PC and RPC have similar effects in terms of mineralization and suggest that RPC also has the potential to be used as a clinically suitable pulp-capping material.

  12. EVALUATION OF LINERBOARD PROPERTIES FROM MALAYSIAN CULTIVATED KENAF SODA-ANTHRAQUINONE PULPS VERSUS COMMERCIAL PULPS

    OpenAIRE

    Ahmad Azizi Mossello; Jalaluddin Harun; Rushdan Ibrahim; Hossien Resalati; Seyed Rashid Fallah Shamsi; Paridah Md Tahir; Mohd Nor Mohad Yusoff

    2010-01-01

    Malaysian cultivated kenaf has been identified as a suitable raw material for linerboard production. This study examines the soda-antraquinone (soda-AQ) pulp of kenaf fibers versus old corrugated container (OCC) and unbleached softwood kraft pulps as the main sources for linerboard production. The results showed significant differences among the pulp properties. The unbleached kraft pulp with very high freeness required high beating to reach an optimized freeness and produced paper with the h...

  13. Wheat Bread with Pumpkin (Cucurbita maxima L.) Pulp as a Functional Food Product

    OpenAIRE

    Renata Różyło; Dariusz Dziki; Anna Jakubczyk; Monika Karaś; Urszula Gawlik-Dziki; Krzysztof Różyło

    2014-01-01

    In this study, a new application of pumpkin pulp in bread production is shown. The aim of this work is to determine the influence of the addition of fresh pumpkin pulp directly into wheat flour on physical, sensorial and biological properties of bread. The bioaccessibility of active compounds was also studied. An increase in the addition of pumpkin pulp from 5 to 20 % (converted to dry matter) caused a decrease of bread volume and increase of crumb hardness and cohesiveness. The sensory chara...

  14. Optimization of pulping conditions of abaca. An alternative raw material for producing cellulose pulp.

    Science.gov (United States)

    Jiménez, L; Ramos, E; Rodríguez, A; De la Torre, M J; Ferrer, J L

    2005-06-01

    The influence of temperature (150-170 degrees C), pulping time (15-45 min) and soda concentration (5-10%) in the pulping of abaca on the yield, kappa, viscosity, breaking length, stretch and tear index of pulp and paper sheets, was studied. Using a factorial design to identify the optimum operating conditions, equations relating the dependent variables to the operational variables of the pulping process were derived that reproduced the former with errors lower than 25%. Using a high temperature, and a medium time and soda concentration, led to pulp that was difficult to bleach (kappa 28.34) but provided acceptable strength-related properties (breaking length 4728 m; stretch 4.76%; tear index 18.25 mN m2/g), with good yield (77.33%) and potential savings on capital equipment costs. Obtaining pulp amenable to bleaching would entail using more drastic conditions than those employed in this work.

  15. Potential of hot water extraction of birch wood to produce high-purity dissolving pulp after alkaline pulping.

    Science.gov (United States)

    Borrega, Marc; Tolonen, Lasse K; Bardot, Fanny; Testova, Lidia; Sixta, Herbert

    2013-05-01

    The potential of hot water extraction of birch wood to produce highly purified dissolving pulp in a subsequent soda-anthraquinone pulping process was evaluated. After intermediate extraction intensities, pulps with low xylan content (3-5%) and high cellulose yield were successfully produced. Increasing extraction intensity further decreased the xylan content in pulp. However, below a xylan content of 3%, the cellulose yield dramatically decreased. This is believed to be due to cleavage of glycosidic bonds in cellulose during severe hot water extractions, followed by peeling reactions during alkaline pulping. Addition of sodium borohydride as well as increased anthraquinone concentration in the pulping liquor increased the cellulose yield, but had no clear effects on pulp purity and viscosity. The low intrinsic viscosity of pulps produced after severe extraction intensities and soda-anthraquinone pulping corresponded to the viscosity at the leveling-off degree of polymerization, suggesting that nearly all amorphous cellulose had been degraded. Copyright © 2012 Elsevier Ltd. All rights reserved.

  16. Enzymatic pulp upgrade for producing high-value cellulose out of a Kraft paper pulp.

    Science.gov (United States)

    Hutterer, Christian; Kliba, Gerhard; Punz, Manuel; Fackler, Karin; Potthast, Antje

    2017-07-01

    The high-yield separation of polymeric parts from wood-derived lignocellulosic material is indispensable in biorefinery concepts. For the separation of cellulose and xylan from hardwood paper pulps to obtain pulps of high cellulose contents, simple alkaline extractions were found to be the most suitable technology, although having certain limitations. These are embodied by residual alkali resistant xylan incorporated in the pulp matrix. Further purification in order to produce pure cellulose with a low uniformity could be achieved selectively degrading residual xylan and depolymerizing the cellulose macromolecules by xylanase and cellulase. The latter help to adjust cellulose chain lengths for certain dissolving pulp grades while reducing the demand for ozone in subsequent TCF bleaching. Experiments applying different commercially available enzyme preparations revealed the dependency of xylanase performance on the residual xylan content in pulps being stimulated by additional cellulase usage. The action of the latter strongly depends on the cellulose allomorphy confirming the impact of the pulp morphology. Hence, the combined application of both types of enzymes offers a high potential for upgrading pulps in order to produce a pure and high-value cellulose product. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Low Temperature Soda-Oxygen Pulping of Bagasse.

    Science.gov (United States)

    Yue, Fengxia; Chen, Ke-Li; Lu, Fachuang

    2016-01-13

    Wood shortages, environmental pollution and high energy consumption remain major obstacles hindering the development of today's pulp and paper industry. Energy-saving and environmental friendly pulping processes are still needed, especially for non-woody materials. In this study, soda-oxygen pulping of bagasse was investigated and a successful soda-oxygen pulping process for bagasse at 100 °C was established. The pulping parameters of choice were under active alkali charge of 23%, maximum cooking temperature 100 °C, time hold at maximum temperature 180 min, initial pressure of oxygen 0.6 MPa, MgSO4 charge 0.5%, and de-pithed bagasse consistency 12%. Properties of the resultant pulp were screened yield 60.9%, Kappa number 14, viscosity 766 dm³/kg, and brightness 63.7% ISO. Similar pulps were also obtained at 110 °C or 105 °C with a cooking time of 90 min. Compared with pulps obtained at higher temperatures (115-125 °C), this pulp had higher screened yield, brightness, and acceptable viscosity, while the delignification degree was moderate. These results indicated that soda-oxygen pulping at 100 °C, the lowest cooking temperature reported so far for soda-oxygen pulping, is a suitable process for making chemical pulp from bagasse. Pulping at lower temperature and using oxygen make it an environmental friendly and energy-saving pulping process.

  18. Low Temperature Soda-Oxygen Pulping of Bagasse

    Directory of Open Access Journals (Sweden)

    Fengxia Yue

    2016-01-01

    Full Text Available Wood shortages, environmental pollution and high energy consumption remain major obstacles hindering the development of today’s pulp and paper industry. Energy-saving and environmental friendly pulping processes are still needed, especially for non-woody materials. In this study, soda-oxygen pulping of bagasse was investigated and a successful soda-oxygen pulping process for bagasse at 100 °C was established. The pulping parameters of choice were under active alkali charge of 23%, maximum cooking temperature 100 °C, time hold at maximum temperature 180 min, initial pressure of oxygen 0.6 MPa, MgSO4 charge 0.5%, and de-pithed bagasse consistency 12%. Properties of the resultant pulp were screened yield 60.9%, Kappa number 14, viscosity 766 dm3/kg, and brightness 63.7% ISO. Similar pulps were also obtained at 110 °C or 105 °C with a cooking time of 90 min. Compared with pulps obtained at higher temperatures (115–125 °C, this pulp had higher screened yield, brightness, and acceptable viscosity, while the delignification degree was moderate. These results indicated that soda-oxygen pulping at 100 °C, the lowest cooking temperature reported so far for soda-oxygen pulping, is a suitable process for making chemical pulp from bagasse. Pulping at lower temperature and using oxygen make it an environmental friendly and energy-saving pulping process.

  19. A randomized clinical trial on the use of medical Portland cement, MTA and calcium hydroxide in indirect pulp treatment.

    Science.gov (United States)

    Petrou, Marina Agathi; Alhamoui, Fadi Alhaddad; Welk, Alexander; Altarabulsi, Mohammed Basel; Alkilzy, Mohammed; H Splieth, Christian

    2014-01-01

    Studies on indirect pulp treatment (IPT) show varying success rates of 73 to 97 %. The necessity of re-opening the cavity and the question of the optimal capping material is still under debate. The aim of this prospective in vivo study was to compare the clinical and microbiological outcomes of mineral trioxide aggregate (MTA), medical Portland cement, and calcium hydroxide on the dentin-pulp complex of permanent and primary teeth treated with two-step IPT. In 86 regular patients (51 % men; 49 % women; age 17.2 years ±13.8), one deep carious lesion each was treated with incomplete caries removal, randomly selected capping with either calcium hydroxide (n = 31), medical Portland cement (29) or white MTA (26), and re-entry (6.3 months ±1.0). Clinical (color, humidity, and consistency of dentin) and microbiological (Lactobacilli/Mutans Strep. counts) parameters were recorded at the first and second treatment. The IPT had a high success rate of 90.3 % regardless of the material used (p = 0.72). The arrested lesions showed consistently darker, dry, and therefore, sclerotic dentine (p Portland cement. The findings of this study could promote the improvement of the IPT as a one-step treatment of deep carious lesions when the remaining demineralized dentin would be sealed with durable restorations.

  20. Extracellular matrix of dental pulp stem cells: Applications in pulp tissue engineering using somatic MSCs

    Directory of Open Access Journals (Sweden)

    Sriram eRavindran

    2014-01-01

    Full Text Available Dental Caries affects approximately 90% of the world’s population. At present, the clinical treatment for dental caries is root canal therapy. This treatment results in loss of tooth sensitivity and vitality. Tissue engineering can potentially solve this problem by enabling regeneration of a functional pulp tissue. Dental pulp stem cells (DPSCs have been shown to be an excellent source for pulp regeneration. However, limited availability of these cells hinders its potential for clinical translation. We have investigated the possibility of using somatic mesenchymal stem cells from other sources for dental pulp tissue regeneration using a biomimetic dental pulp extracellular matrix (ECM incorporated scaffold. Human periodontal ligament stem cells (PDLSCs and human bone marrow stromal cells (HMSCs were investigated for their ability to differentiate towards an odontogenic lineage. In vitro real-time PCR results coupled with histological and immunohistochemical examination of the explanted tissues confirmed the ability of PDLSCs and HMSCs to form a vascularized pulp-like tissue. These findings indicate that the dental pulp stem derived ECM scaffold stimulated odontogenic differentiation of PDLSCs and HMSCs without the need for exogenous addition of growth and differentiation factors. This study represents a translational perspective toward possible therapeutic application of using a combination of somatic stem cells and extracellular matrix for pulp regeneration.

  1. REPLACEMENT OF SOFTWOOD KRAFT PULP WITH ECF-BLEACHED BAMBOO KRAFT PULP IN FINE PAPER

    Directory of Open Access Journals (Sweden)

    Guanglei Zhao

    2010-06-01

    Full Text Available Non-wood fibers such as bamboo and wheat straw have been playing important roles in the pulp and paper industry in China. In this study an ECF-bleached bamboo kraft pulp was compared with a bleached softwood kraft pulp (NBSK as the reinforcement pulp in fine paper production. Areas that were examined include the refining of pure fibers, influence of bamboo on dewatering, retention, and sizing. The influence of bamboo kraft pulp as a part of a furnish replacing NBSK was compared as well. Results show that fiber shortening was more prominent with bamboo when refined. This resulted in a higher amount of fines, and addition wet-end chemicals may be required to compensate. Handsheets with bamboo as a reinforcement fiber showed similar mechanical and optical properties to handsheets containing NBSK.

  2. Bio-refinery system in a pulp mill for methanol production with comparison of pressurized black liquor gasification and dry gasification using direct causticization

    International Nuclear Information System (INIS)

    Naqvi, Muhammad; Yan, Jinyue; Dahlquist, Erik

    2012-01-01

    Black liquor gasification (BLG) for bio-fuel or electricity production at the modern pulp mills is a field in continuous evolution and the efforts are considerably driven by the climate change, fuel security, and renewable energy. This paper evaluates and compares two BLG systems for methanol production: (i) oxygen blown pressurized thermal BLG; and (ii) dry BLG with direct causticization, which have been regarded as the most potential technology candidates for the future deployment. A key objective is to assess integration possibilities of BLG technologies with the reference Kraft pulp mill producing 1000 air dried tonnes (ADt) pulp/day replacing conventional recovery cycle. The study was performed to compare the systems’ performance in terms of potential methanol production, energy efficiency, and potential CO 2 reductions. The results indicate larger potential of black liquor conversion to methanol from the pressurized BLG system (about 77 million tonnes/year of methanol) than the dry BLG system (about 30 million tonnes/year of methanol) utilizing identical amount of black liquor available worldwide (220 million tDS/year). The potential CO 2 emissions reduction from the transport sector is substantially higher in pressurized BLG system (117 million tonnes/year CO 2 reductions) as compared to dry BLG system (45 million tonnes/year CO 2 reductions). However, the dry BLG system with direct causticization shows better results when considering consequences of additional biomass import. In addition, comparison of methanol production via BLG with other bio-refinery products, e.g. hydrogen, dimethyl ether (DME) and bio-methane, has also been discussed.

  3. The number of bleaching sessions influences pulp tissue damage in rat teeth.

    Science.gov (United States)

    Cintra, Luciano Tavares Angelo; Benetti, Francine; da Silva Facundo, Aguinaldo Cândido; Ferreira, Luciana Louzada; Gomes-Filho, João Eduardo; Ervolino, Edilson; Rahal, Vanessa; Briso, André Luiz Fraga

    2013-12-01

    Hydrogen peroxide tooth bleaching is claimed to cause alterations in dental tissue structures. This study investigated the influence of the number of bleaching sessions on pulp tissue in rats. Male Wistar rats were studied in 5 groups (groups 1S-5S) of 10 each, which differed by the number (1-5) of bleaching sessions. In each session, the animals were anesthetized, and 35% hydrogen peroxide gel was applied to 3 upper right molars. Two days after the experimental period, the animals were killed, and their jaws were processed for light microscope evaluation. Pulp tissue reactions were scored as follows: 1, no or few inflammatory cells and no reaction; 2, session, necrotic tissue in the pulp horns and underlying inflammatory changes were observed. The extent and intensity of these changes increased with the number of bleaching sessions. After 5 sessions, the changes included necrotic areas in the pulp tissue involving the second third of the radicular pulp and intense inflammation in the apical third. The number of bleaching sessions directly influenced the extent of pulp damage. Copyright © 2013 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  4. Swedish Pulp Mill Biorefineries. A vision of future possibilities

    Energy Technology Data Exchange (ETDEWEB)

    Berntsson, Thore (Chamers Univ. of Technology, Goeteborg (Sweden)); Axegaard, Peter; Backlund, Birgit; Samuelsson, Aasa; Berglin, Niklas; Lindgren, Karin (STFI-Packforsk, Stockholm (Sweden))

    2008-07-01

    Today, modern science could make it possible to develop techniques for refining almost the whole wood-matter, pulp mill side streams and bark compounds into platform chemicals, electricity, high quality fuels and structured feed-stock for chemicals and materials. The major challenge is to convert the state of basic scientific knowledge into industrial practise. Our definition of an integrated biorefinery is: 'Full utilization of the incoming biomass and other raw materials for simultaneous and economically optimized production of fibres, chemicals and energy'. Examples of products from a pulp mill biorefinery are: Chemicals and Materials (Phenols, adhesives, carbon fibres, activated carbon, binders, barriers, adhesives, antioxidants, surfactants, chelants, solvents, adhesives surfactants, descaling agents, specialty polymers, pharmaceuticals, nutraceuticals, cosmetics etc., Biofuels (pellets, lignin fuel, methanol, DME, ethanol etc), Electricity (BLGCC, condensing power etc.). The new or increased amounts of traditional products can be made from internal and/or external biomass. Three different levels can be identified: A high degree of energy saving in future mills, especially chemical pulp mills, will lead to large amounts of excess internal biomass which can be transferred to products mentioned above, Components in e.g. the black liquor, forest residues and bark can be upgraded to more valuable ones and the energy balance of the mill is kept through fuel import, wholly or partly depending on the level of mill energy efficiency. This imported fuel can be biomass or other types. External (imported) biomass (in some cases together with excess internal biomass) can be upgraded using synergy effects of docking this upgrading to a pulp mill. Electricity has been included as one of the possible biorefinery products. The electricity production in a mill can be increased in several ways which cannot be directly considered as biorefineries, e.g. recovery boiler

  5. Enzymes improve ECF bleaching of pulp

    Directory of Open Access Journals (Sweden)

    Lachenal, D.

    2006-07-01

    Full Text Available The delignification efficiency of different laccase enzymes was examined on the eucalyptus Kraft pulp. The laccase enzyme from Trametes versicolor showing the highest delignification efficiency was selected and used in the elemental chlorine-free bleaching sequence for improving the pulp bleachability. An appreciable reduction in chlorine dioxide consumption was also obtained. Further reduction in chlorine dioxide consumption was obtained when the same laccase treated pulp was subjected to an acid treatment after the extraction stage followed by the DEPD sequence. Elemental-chlorine free bleaching was also performed using the xylanase-laccase treated pulp. Xylanase treatment was incorporated to the laccase mediator system in the elemental-chlorine free bleaching both sequentially and simultaneously. The bleaching sequence DEPD followed and in both the cases, the reduction in chlorine dioxide consumption was greater in comparison to the control. The chlorine dioxide consumption was reduced further when xylanase-laccase treated pulp was given an additional acid treatment. The final pulp properties of the treated pulps were comparable to the control pulp.

  6. Human dental pulp stem cells with highly angiogenic and neurogenic potential for possible use in pulp regeneration.

    Science.gov (United States)

    Nakashima, Misako; Iohara, Koichiro; Sugiyama, Masahiko

    2009-01-01

    Dental caries is a common public health problem, causing early loss of dental pulp and resultant tooth loss. Dental pulp has important functions to sustain teeth providing nutrient and oxygen supply, innervation, reactionary/reparative dentin formation and immune response. Regeneration of pulp is an unmet need in endodontic therapy, and angiogenesis/vasculogenesis and neurogenesis are critical for pulp regeneration. Permanent and deciduous pulp tissue is easily available from teeth after extraction without ethical issues and has potential for clinical use. In this review, we introduce some stem cell subfractions, CD31(-)/CD146(-) SP cells and CD105(+) cells with high angiogenic and neurogenic potential, derived from human adult dental pulp tissue. Potential utility of these cells is addressed as a source of cells for treatment of cerebral and limb ischemia and pulp inflammation complete with angiogenesis and vasculogenesis.

  7. Eucalyptus kraft pulp production: Thermogravimetry monitoring

    International Nuclear Information System (INIS)

    Barneto, Agustin G.; Vila, Carlos; Ariza, Jose

    2011-01-01

    Highlights: → Thermogravimetric analysis can be used to monitor the pulping process in a pulp mill. → ECF bleaching process affects the crystalline cellulose volatilization. → The fibre size has an influence on composition and thermal behavior of pulp. - Abstract: Under oxidative environment the thermal degradation of lignocellulosic materials like wood or pulp is sensitive to slight composition changes. For this, in order to complement the chemical and X-ray diffraction results, thermogravimetric analyses (TGA) were used to monitor pulp production in a modern pulp mill. Runs were carried out on crude, oxygen delignified and bleached pulps from three eucalyptus woods from different species and geographical origins. Moreover, with the modeling of thermogravimetric data, it was possible to obtain an approximate composition of samples which includes crystalline and amorphous cellulose. TGA results show that pulping has an intensive effect on bulk lignin and hemicellulose, but it has limited influence on the removal of these substances when they are linked to cellulose microfibril. The stages of oxygen delignification and bleaching, based in chlorine dioxide and hydrogen peroxide, increase the crystalline cellulose volatilization rate. These changes are compatible with a more crystalline microfibril. The influence of the fibre size on pulp composition, crystallinity and thermal degradation behavior was observed.

  8. CAPS OpenACC Compilers: Performance and Portability

    CERN Multimedia

    CERN. Geneva

    2013-01-01

    The announcement late 2011 of the new OpenACC directive-based programming standard supported by CAPS, CRAY and PGI compilers has open up the door to more scientific applications that can be ported on many-core systems. Following a porting methodology, this talk will first review the principles of programming with OpenACC and then the advanced features available in the CAPS compilers to further optimize OpenACC applications: library integration, tuning directives with auto-tune mechanisms to build applications adaptive to different GPUs. CAPS compilers use hardware vendors' backends such as NVIDIA CUDA and OpenCL making them the only OpenACC compilers supporting various many-core architectures. About the speaker Stéphane Bihan is co-funder and currently Director of Sales and Marketing at CAPS enterprise. He has held several R&D positions in companies such as ARC international plc in London, Canon Research Center France, ACE compiler experts in Amsterdam and the INRIA r...

  9. PULP DEMAND IN THE INTERNATIONAL MARKET

    Directory of Open Access Journals (Sweden)

    Edmilson Santos Cruz

    2003-01-01

    Full Text Available This study aimed at analyzing the international pulp market, taking into account themain exporting countries and importing regions, with the objective of estimating, for each market, theown-price and cross-price elasticity in relation to the demand of the pulp, differentiated for country oforigin. The model considers that imports are differentiated by origin; therefore they are not perfect substitutes. The demand from Europe, North America and the Rest of the World for the pulp from theUnited States,Canada, Sweden, Finland, Portugal and Brazil was inelastic. The Asian demand for thissome pulp was elastic. Europe and the Rest of the World showed negative cross-price elasticity, i. e.,and the imported pulp from other countries are complementary products. North America and Asiashowed positive crow-price elasticity, i. e., they consider the pulp produced in other countries assubstitute products. The net effect of the variation on the price of pulp in a country h, over the amountof pulp that goes to the region i depends on the matching of values related to the elasticity ofsubstitution and the price elasticity of the total demand.

  10. Pulp regeneration: Current approaches and future challenges

    Directory of Open Access Journals (Sweden)

    Jingwen eYANG

    2016-03-01

    Full Text Available Regenerative endodontics aims to replace inflamed/necrotic pulp tissues with regenerated pulp-like tissues to revitalize teeth and improve life quality. Pulp revascularization case reports, which showed successful clinical and radiographic outcomes, indicated the possible clinical application of pulp regeneration via cell homing strategy. From a clinical point of view, functional pulp-like tissues should be regenerated with the characterization of vascularization, re-innervation, and dentin deposition with a regulated rate similar to that of normal pulp. Efficient root canal disinfection and proper size of the apical foramen are the two requisite preconditions for pulp regeneration. Progress has been made on pulp regeneration via cell homing strategies. This review focused on the requisite preconditions and cell homing strategies for pulp regeneration. In addition to the traditionally used mechanical preparation and irrigation, antibiotics, irrigation assisted with EndoVac apical negative-pressure system, and ultrasonic and laser irradiation are now being used in root canal disinfection. In addition, pulp-like tissues could be formed with the apical foramen less than 1 mm, although more studies are needed to determine the appropriate size. Moreover, signaling molecules including stromal cell derived factor (SDF-1α, basic Fibroblast Growth Factor (bFGF, Platelet Derived Growth Factor (PDGF, stem cell factor (SCF, and Granulocyte Colony-Stimulating Factor (G-CSF were used to achieve pulp-like tissue formation via a cell homing strategy. Studies on the cell sources of pulp regeneration might give some indications on the signaling molecular selection. The active recruitment of endogenous cells into root canals to regenerate pulp-like tissues is a novel concept that may offer an unprecedented opportunity for the near-term clinical translation of current biology-based therapies for dental pulp regeneration.

  11. Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration

    OpenAIRE

    Hu, Lei; Gao, Zhenhua; Xu, Junji; Zhu, Zhao; Fan, Zhipeng; Zhang, Chunmei; Wang, Jinsong; Wang, Songlin

    2017-01-01

    Endodontic regeneration shows promise in treating dental pulp diseases; however, no suitable scaffolds exist for pulp regeneration. Acellular natural extracellular matrix (ECM) is a favorable scaffold for tissue regeneration since the anatomical structure and ECM of the natural tissues or organs are well-preserved. Xenogeneic ECM is superior to autologous or allogeneic ECM in tissue engineering for its unlimited resources. This study investigated the characteristics of decellularized dental p...

  12. Low Temperature Soda-Oxygen Pulping of Bagasse

    OpenAIRE

    Fengxia Yue; Ke-Li Chen; Fachuang Lu

    2016-01-01

    Wood shortages, environmental pollution and high energy consumption remain major obstacles hindering the development of today’s pulp and paper industry. Energy-saving and environmental friendly pulping processes are still needed, especially for non-woody materials. In this study, soda-oxygen pulping of bagasse was investigated and a successful soda-oxygen pulping process for bagasse at 100 °C was established. The pulping parameters of choice were under active alkali charge of 23%, maximum coo...

  13. Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study.

    Science.gov (United States)

    Nakashima, Misako; Iohara, Koichiro; Murakami, Masashi; Nakamura, Hiroshi; Sato, Yayoi; Ariji, Yoshiko; Matsushita, Kenji

    2017-03-09

    Experiments have previously demonstrated the therapeutic potential of mobilized dental pulp stem cells (MDPSCs) for complete pulp regeneration. The aim of the present pilot clinical study is to assess the safety, potential efficacy, and feasibility of autologous transplantation of MDPSCs in pulpectomized teeth. Five patients with irreversible pulpitis were enrolled and monitored for up to 24 weeks following MDPSC transplantation. The MDPSCs were isolated from discarded teeth and expanded based on good manufacturing practice (GMP). The quality of the MDPSCs at passages 9 or 10 was ascertained by karyotype analyses. The MDPSCs were transplanted with granulocyte colony-stimulating factor (G-CSF) in atelocollagen into pulpectomized teeth. The clinical and laboratory evaluations demonstrated no adverse events or toxicity. The electric pulp test (EPT) of the pulp at 4 weeks demonstrated a robust positive response. The signal intensity of magnetic resonance imaging (MRI) of the regenerated tissue in the root canal after 24 weeks was similar to that of normal dental pulp in the untreated control. Finally, cone beam computed tomography demonstrated functional dentin formation in three of the five patients. Human MDPSCs are safe and efficacious for complete pulp regeneration in humans in this pilot clinical study.

  14. Viscoelastic Properties of Dental Pulp Tissue and Ramifications on Biomaterial Development for Pulp Regeneration.

    Science.gov (United States)

    Erisken, Cevat; Kalyon, Dilhan M; Zhou, Jian; Kim, Sahng G; Mao, Jeremy J

    2015-10-01

    A critical step in biomaterial selection effort is the determination of material as well as the biological properties of the target tissue. Previously, the selection of biomaterials and carriers for dental pulp regeneration has been solely based on empirical experience. In this study, first, the linear viscoelastic material functions and compressive properties of miniature pig dental pulp were characterized using small-amplitude oscillatory shear and uniaxial compression at a constant rate. They were then compared with the properties of hydrogels (ie, agarose, alginate, and collagen) that are widely used in tissue regeneration. The comparisons of the linear viscoelastic material functions of the native pulp tissue with those of the 3 hydrogels revealed the gel-like behavior of the pulp tissue over a relatively large range of time scales (ie, over the frequency range of 0.1-100 rps). At the constant gelation agent concentration of 2%, the dynamic properties (ie, storage and loss moduli and the tanδ) of the collagen-based gel approached those of the native tissue. Under uniaxial compression, the peak normal stresses and compressive moduli of the agarose gel were similar to those of the native tissue, whereas alginate and collagen exhibited significantly lower compressive properties. The linear viscoelastic and uniaxial compressive properties of the dental pulp tissue reported here should enable the more appropriate selection of biogels for dental pulp regeneration via the better tailoring of gelation agents and their concentrations to better mimic the dynamic and compressive properties of native pulp tissue. Copyright © 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  15. PRODUCTION OF DISSOLVING GRADE PULP FROM ALFA

    Directory of Open Access Journals (Sweden)

    Baya Bouiri

    2010-02-01

    Full Text Available Alfa, also known as Stipa tenacissimaI or “halfa”, is grown in North Africa and south Spain. Due to its short fiber length, paper made from alfa pulp retains bulk and takes block letters well. In this study alfa was evaluated for bleached pulp production. Two cellulose pulps with different chemical compositions were pulped by a conventional kraft process. One sample was taken from the original alfa material and another from alfa that had been pretreated by diluted acid. The pulp produced from the pretreated alfa was bleached by the elemental-chlorine-free sequences DEPD and DEDP. The yield, Kappa number, brightness, and α- cellulose content of bleached and unbleached pulps were evaluated. The results showed that during the chemical pulping process, treated alfa cooked more easily than the original alfa. The treated alfa pulp also showed very good bleaching, reaching a brightness level of 94.8% ISO with a yield of 93.6% at an α-cellulose content 96.8(% with a DEDP bleaching sequence, compared to 83.2% ISO brightness level, 92.8% yield, and 95.1% α-cellulose content for bleached pulp with a DEPD bleaching sequence. Therefore, this alfa material could be considered as a worthwhile choice for cellulosic fiber supply.

  16. Alkaline pulping of some eucalypts from Sudan.

    Science.gov (United States)

    Khristova, P; Kordsachia, O; Patt, R; Dafaalla, S

    2006-03-01

    Four eucalypts (Eucalyptus camaldulensis, Eucalyptus microtheca, Eucalyptus tereticornis and Eucalyptus citriodora) grown in Sudan were examined for their suitability for pulping and papermaking with different alkaline methods. Their physical, morphological and chemical characteristics are reported. The pulping trials with E. citriodora and E. tereticornis were carried out using the kraft-AQ, soda-AQ, modified AS/AQ (ASA), ASAM and kraft methods. For the other two species, only the ASAM and the kraft process were applied. ASAM pulping gave the best results in terms of yield, degree of delignification, mechanical and optical pulp properties. The best pulps, obtained in kraft and ASAM cooking of E. citriodora, were bleached to 88% ISO brightness in a totally chlorine free bleaching sequence (OQ1O/PQ2P). The bleached pulps, especially the ASAM pulp, showed good papermaking properties and would be suitable for manufacture of writing and printing grades of paper.

  17. Cervical Cap

    Science.gov (United States)

    ... Videos for Educators Search English Español The Cervical Cap KidsHealth / For Teens / The Cervical Cap What's in ... Call the Doctor? Print What Is a Cervical Cap? A cervical cap is a small cup made ...

  18. Kinetic modeling of formic acid pulping of bagasse.

    Science.gov (United States)

    Tu, Qiliang; Fu, Shiyu; Zhan, Huaiyu; Chai, Xinsheng; Lucia, Lucian A

    2008-05-14

    Organic solvent or organosolv pulping processes are alternatives to soda or kraft pulping to delignify lignocellulosic materials for the production of paper pulp. Formic acid, a typical organosolv system, has been presently examined under atmospheric pressure to pulp bagasse fibers. It was shown that efficient bagasse pulping was achieved when the formic acid concentration was limited to 90% (v/v). A statistical kinetic model based on the experimental results for the delignification of bagasse during formic acid pulping was developed that can be described as follows: D (delignification) = 0.747 x C(formicacid) (1.688) x (1 - e(-0.05171t)), an equation that can be used to predict the lignin content in formic acid during the pulping process. The delignification of bagasse by 90% formic acid was almost completed after approximately 80 min, while extended pulping did not improve the delignification but tended to degrade the carbohydrates in bagasse, especially the hemicelluloses, which were rapidly hydrolyzed at the onset of pulping.

  19. Chapter 6: Prehydrolysis Pulping with Fermentation Coproducts

    Science.gov (United States)

    T.H. Wegner; C.J. Houtman; A.W. Rudie; B.L. Illman; P.J. Ince; E.M. Bilek; T.W. Jeffries

    2013-01-01

    Although the term “integrateed biorefinery” is new, the concept has long been familiar to the pulp and paper industry, where processes include biomass boilers providing combined heat and power, and byproducts of pulping include turpentine, fatty acids and resin acids. In the dominant kraft (or sulfate) pulping process, dissolved lignin and chemicals from the pulp...

  20. Expression of high mobility group box 1 in inflamed dental pulp and its chemotactic effect on dental pulp cells

    International Nuclear Information System (INIS)

    Zhang, Xufang; Jiang, Hongwei; Gong, Qimei; Fan, Chen; Huang, Yihua; Ling, Junqi

    2014-01-01

    Highlights: • HMGB1 translocated from nucleus to cytoplasm during dental pulp inflammation. • HMGB1and its receptor RAGE were up-regulated in hDPCs under LPS stimulation. • HMGB1 enhanced hDPCs migration and induces cytoskeleton reorganization. • HMGB1 may play a critical role in dental pulp repair during inflamed state. - Abstract: High mobility group box 1 protein (HMGB1) is a chromatin protein which can be released extracellularly, eliciting a pro-inflammatory response and promoting tissue repair process. This study aimed to examine the expression and distribution of HMGB1 and its receptor RAGE in inflamed dental pulp tissues, and to assess its effects on proliferation, migration and cytoskeleton of cultured human dental pulp cells (DPCs). Our data demonstrated that cytoplasmic expression of HMGB1 was observed in inflamed pulp tissues, while HMGB1 expression was confined in the nuclei in healthy dental pulp. The mRNA expression of HMGB1 and RAGE were significantly increased in inflamed pulps. In in vitro cultured DPCs, expression of HMGB1 in both protein and mRNA level was up-regulated after treated with lipopolysaccharide (LPS). Exogenous HMGB1 enhanced DPCs migration in a dose-dependent manner and induced the reorganization of f-actin in DPCs. Our results suggests that HMGB1 are not only involved in the process of dental pulp inflammation, but also play an important role in the recruitment of dental pulp stem cells, promoting pulp repair and regeneration

  1. Expression of high mobility group box 1 in inflamed dental pulp and its chemotactic effect on dental pulp cells

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Xufang, E-mail: xufang.zhang@student.qut.edu.au [Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055 (China); Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059 (Australia); Jiang, Hongwei, E-mail: jianghw@163.com [Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055 (China); Gong, Qimei, E-mail: gongqmei@gmail.com [Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055 (China); Fan, Chen, E-mail: c3.fan@student.qut.edu.au [Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059 (Australia); Huang, Yihua, E-mail: enu0701@163.com [Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055 (China); Ling, Junqi, E-mail: lingjq@mail.sysu.edu.cn [Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055 (China)

    2014-08-08

    Highlights: • HMGB1 translocated from nucleus to cytoplasm during dental pulp inflammation. • HMGB1and its receptor RAGE were up-regulated in hDPCs under LPS stimulation. • HMGB1 enhanced hDPCs migration and induces cytoskeleton reorganization. • HMGB1 may play a critical role in dental pulp repair during inflamed state. - Abstract: High mobility group box 1 protein (HMGB1) is a chromatin protein which can be released extracellularly, eliciting a pro-inflammatory response and promoting tissue repair process. This study aimed to examine the expression and distribution of HMGB1 and its receptor RAGE in inflamed dental pulp tissues, and to assess its effects on proliferation, migration and cytoskeleton of cultured human dental pulp cells (DPCs). Our data demonstrated that cytoplasmic expression of HMGB1 was observed in inflamed pulp tissues, while HMGB1 expression was confined in the nuclei in healthy dental pulp. The mRNA expression of HMGB1 and RAGE were significantly increased in inflamed pulps. In in vitro cultured DPCs, expression of HMGB1 in both protein and mRNA level was up-regulated after treated with lipopolysaccharide (LPS). Exogenous HMGB1 enhanced DPCs migration in a dose-dependent manner and induced the reorganization of f-actin in DPCs. Our results suggests that HMGB1 are not only involved in the process of dental pulp inflammation, but also play an important role in the recruitment of dental pulp stem cells, promoting pulp repair and regeneration.

  2. Effect of white mineral trioxide aggregate compared with biomimetic carbonated apatite on dentine bridge formation and inflammatory response in a dental pulp model.

    Science.gov (United States)

    Danesh, F; Vahid, A; Jahanbani, J; Mashhadiabbas, F; Arman, E

    2012-01-01

      To evaluate the effects of apatite precipitation on the biocompatibility and hard tissue induction properties of white mineral trioxide aggregate (WMTA) in a dental pulp model.   Pulp exposures were created on the axial walls of 32 sound canine teeth of eight dogs. Four additional sound teeth served as controls. The pulps were capped either with WMTA or apatite derivatives [biomimetic carbonated apatite (BCAp)] in the interaction of WMTA with a synthetic tissue fluid and restored with zinc oxide-eugenol cement. After 7 and 70 days, the animals were killed, and the histological specimens taken from the teeth were stained with haematoxylin and eosin for histomorphological evaluation. The Brown and Brenn technique was employed to stain bacteria. The data were subjected to nonparametric Kruskall-Wallis analysis and Mann-Whitney U_tests.   Biomimetic carbonated apatite did not induce hard tissue bridge formation. WMTA performed significantly better than BCAp in this respect at both periods (P 0.05).   White mineral trioxide aggregate induced hard tissue formation via a mechanism other than that postulated via apatite formation. © 2011 International Endodontic Journal.

  3. Pulp mill as an energy producer

    International Nuclear Information System (INIS)

    Kaulamo, O.

    1998-01-01

    The recovery boilers of pulp mills are today the most significant producers of wood energy. The power-to-heat ratio of the power plant process, i.e., power yield, is poor in existing applications. In the study, an alternative of improving the power yield of conventional pulp mills significantly was studied by applying solutions used in power plants to a pulp mill. Extensive conversion of wood energy into electricity is possible only in the recovery boiler of the pulp mill and in a large combustion boiler of bark, wood waste and wood chips integrated to this boiler. Hence, the harvest and transports of wood raw materials, i.e. pulp wood and energy wood, are integrated, and the fraction going to cook and the energy wood fraction are separated at the pulp mill. The method guarantees competitive supply of energy wood. As a result a SELLUPOWER mill was designed, where the recovery boiler combusting black liquor and the large power plant boiler combusting energy wood are integrated to one unit and constructed to a power plant process with a high power-to-heat ratio. Necessary technical solutions, project costs and economical feasibility compared to a conventional pulp mill were determined, and the effect of different production-economical parameters was also studied. (orig.)

  4. Immunolocalization of bone-resorptive cytokines in rat pulp and periapical lesions following surgical pulp exposure.

    Science.gov (United States)

    Tani-Ishii, N; Wang, C Y; Stashenko, P

    1995-08-01

    The bone-resorptive cytokines interleukin 1 (IL-1) and tumor necrosis factor (TNF) have been implicated in the pathogenesis of many chronic inflammatory diseases, including pulpitis and apical periodontitis.To further elucidate their role in these disorders, we have identified cells that express IL-1 alpha and TNF alpha in infected pulps and in developing rat periapical lesions after surgical pulp exposure. As detected by immunohistochemistry, IL-1 alpha- and TNF alpha-positive cells were present as early as 2 days after pulp exposure in both the pulp and periapical region. The numbers of cytokine-expressing cells increased up to day 4 in the pulp and up to day 30 in the periapex. In contrast, cells expressing IL-1 beta and TNF beta, the homologous forms of these mediators, were not found in pulp or periapical lesions during this period. Cells expressing IL-1 alpha and TNF alpha were identified primarily as macrophages and fibroblasts, with occasional staining of polymorphonuclear leukocytes. Osteoblasts and osteoclasts were also positive, whereas lymphocytes were negative. In general, cytokine-expressing cells were located proximal to abscesses and the root apex. These findings demonstrate that cells that express bone-resorptive cytokines IL-1 alpha and TNF alpha are present immediately after pulp exposure in this model, which supports the hypothesis that these mediators play a key role in pulpal and periapical pathogenesis, including the concomitant bone destruction. They also indicate that both resident connective tissue cells as well as infiltrating cells express bone-resorptive cytokines in response to infection in these lesions.

  5. Evaluating pulp stiffness from fibre bundles by ultrasound

    Science.gov (United States)

    Karppinen, Timo; Montonen, Risto; Määttänen, Marjo; Ekman, Axel; Myllys, Markko; Timonen, Jussi; Hæggström, Edward

    2012-06-01

    A non-destructive ultrasonic tester was developed to measure the stiffness of pulp bundles. The mechanical properties of pulp are important when estimating the behaviour of paper under stress. Currently available pulp tests are tedious and alter the fibres structurally and mechanically. The developed tester employs (933 ± 15) kHz tweezer-like ultrasonic transducers and time-of-flight measurement through (9.0 ± 2.5) mm long and (0.8 ± 0.1) mm thick fibre bundles kept at (19.1 ± 0.4) °C and (62 ± 1)% RH. We determined the stiffness of soft wood pulps produced by three kraft pulping modifications: standard kraft pulp, (5.2 ± 0.4) GPa, prehydrolysis kraft pulp, (4.3 ± 0.4) GPa, and alkali extracted prehydrolysis kraft pulp, (3.3 ± 0.4) GPa. Prehydrolysis and alkali extraction processes mainly lowered the hemicellulose content of the pulps, which essentially decreased the fibre-wall stiffness hence impairing the stiffness of the fibre networks. Our results indicate that the method allows ranking of pulps according to their stiffness determined from bundle-like samples taken at an early phase of the papermaking process.

  6. North Polar Cap

    Science.gov (United States)

    2004-01-01

    [figure removed for brevity, see original site] This week we will be looking at five examples of laminar wind flow on the north polar cap. On Earth, gravity-driven south polar cap winds are termed 'catabatic' winds. Catabatic winds begin over the smooth expanse of the cap interior due to temperature differences between the atmosphere and the surface. Once begun, the winds sweep outward along the surface of the polar cap toward the sea. As the polar surface slopes down toward sealevel, the wind speeds increase. Catabatic wind speeds in the Antartic can reach several hundreds of miles per hour. In the images of the Martian north polar cap we can see these same type of winds. Notice the streamers of dust moving downslope over the darker trough sides, these streamers show the laminar flow regime coming off the cap. Within the trough we see turbulent clouds of dust, kicked up at the trough base as the winds slow down and enter a chaotic flow regime. The horizontal lines in these images are due to framelet overlap and lighting conditions over the bright polar cap. Image information: VIS instrument. Latitude 86.5, Longitude 64.5 East (295.5 West). 40 meter/pixel resolution. Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time. NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS) was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing. The THEMIS investigation is led by Dr. Philip Christensen

  7. Diabetes induces metabolic alterations in dental pulp.

    Science.gov (United States)

    Leite, Mariana Ferreira; Ganzerla, Emily; Marques, Márcia Martins; Nicolau, José

    2008-10-01

    Diabetes can interfere in tissue nutrition and can impair dental pulp metabolism. This disease causes oxidative stress in cells and tissues. However, little is known about the antioxidant system in the dental pulp of diabetics. Thus, it would be of importance to study this system in this tissue in order to verify possible alterations indicative of oxidative stress. The aim of this study was to evaluate some parameters of antioxidant system of the dental pulp of healthy (n = 8) and diabetic rats (n = 8). Diabetes was induced by streptozotocin in rats. Six weeks after diabetes induction, a pool of the dental pulp of the 4 incisors of each rat (healthy and diabetic) was used for the determination of total protein and sialic acid concentrations and catalase and peroxidase activities. Data were compared by a Student t test (p pulps from both groups presented similar total protein concentrations and peroxidase activity. Dental pulps of diabetic rats exhibited significantly lower free, conjugated, and total sialic acid concentrations than those of control tissues. Catalase activity in diabetic dental pulps was significantly enhanced in comparison with that of control pulps. The result of the present study is indicative of oxidative stress in the dental pulp caused by diabetes. The increase of catalase activity and the reduction of sialic acid could be resultant of reactive oxygen species production.

  8. Biogas generation apple pulp.

    Science.gov (United States)

    Llaneza Coalla, H; Blanco Fernández, J M; Morís Morán, M A; López Bobo, M R

    2009-09-01

    In view of the pressing problem that appears in our region (Asturias, north of Spain) with the residues from the cider production, it was decided to test this kind of material as a co-substrate joint with slaughterhouse waste in a laboratory unit. The anaerobic digestion of apple pulp was investigated for biogas production. This paper presents the results where apple pulp was co-digested with slaughterhouse waste (pig intestine and bovine stomach content) in a biogas laboratory unit (10 l CSTR reactor). The production of biogas has reached very satisfactory values during the whole test (0.8m(3)kg(-1)OTS), verifying that the process is kept in stable conditions of pH (near 8.0), and the volatile fatty acids was always underneath 3000 mg/l, when the pulp amount was lower than 100g in mesophilic conditions. The fat concentration into the digester remained always below the value that causes inhibition of the methanogenic bacteria, 500 mg/l. Finally, methane concentration (77-80%) and H(2)S concentration (400 ppm) in the biogas, they were similar to those obtained when the test was run out in the absence of apple pulp. The process efficiency with respect to COD removal was high, near 80% of the total COD. Finally, inhibitory effects of methanogenic bacteria were observed when pulp concentration was around 10% in the input material.

  9. Kraft pulping of industrial wood waste

    Science.gov (United States)

    Aziz. Ahmed; Masood. Akhtar; Gary C. Myers; Gary M. Scott

    1998-01-01

    Most of the approximately 25 to 30 million tons of industrial wood waste generated in the United States per year is burned for energy and/or landfilled. In this study, kraft pulp from industrial wood waste was evaluated and compared with softwood (loblolly pine, Douglas-fir) and hardwood (aspen) pulp. Pulp bleachability was also evaluated. Compared to loblolly pine...

  10. Effectiveness evaluation of three RCRA caps at the Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    Shevenell, L.A. [Nevada Bureau of Mines and Geology, Reno, NV (United States); Goldstrand, P.M. [Univ. of Tennessee, Knoxville, TN (United States). Dept. of Geological Sciences

    1994-01-01

    Because installation of Resource Conservation and Recovery Act (RCRA)- engineered caps is costly, it is prudent to evaluate the effectiveness of this procedure for hydrologically isolating contaminants. The objective for installation of five-part engineered caps at the Y-12 Plant was to (1) satisfy the regulatory compliance issues, (2) minimize the risk of direct contact with the wastes, and (3) reduce rainfall infiltration. Although the original objectives of installing the caps were not to alter groundwater flow, a potential effect of reducing infiltration is to minimize leaching, thus retarding groundwater contaminant migration from the site. Hence, cap effectiveness with respect to reduced groundwater contaminant migration is evaluated using groundwater data in this report. Based on the available data at the Y-12 capped areas, evaluation of cap effectiveness includes studying water level and chemical variability in nearby monitoring wells. Three caps installed during 1989 are selected for evaluation in this report. These caps are located in three significantly different hydrogeologic settings: overlying a karst aquifer (Chestnut Ridge Security Pits [CRSP]), overlying shales located on a hill slope (Oil Landfarm Waste Management Area [OLWMA]), and overlying shales in a valley floor which is a site of convergent groundwater flow (New Hope Pond [NHP]). Presumably, the caps have been effective in minimizing risk of direct contact with the wastes and halting direct rainfall infiltration into the sites over the extent of the capped areas, but no evidence is presented in this report to directly demonstrate this. The caps installed over the three sites appear to have had a minimal effect on groundwater contaminant migration from the respective sites. Following cap construction, no changes in the configuration of the water table were observed. Migration of contaminant plumes occurred at all three sites, apparently without regard to the timing of cap installation.

  11. Potassium hydroxide pulping of rice straw in biorefinery initiatives.

    Science.gov (United States)

    Jahan, M Sarwar; Haris, Fahmida; Rahman, M Mostafizur; Samaddar, Purabi Rani; Sutradhar, Shrikanta

    2016-11-01

    Rice straw is supposed to be one of the most important lignocellulosic raw materials for pulp mill in Asian countries. The major problem in rice straw pulping is silica. The present research is focused on the separation of silica from the black liquor of rice straw pulping by potassium hydroxide (KOH) and pulp evaluation. Optimum KOH pulping conditions of rice straw were alkali charge 12% as NaOH, cooking temperature 150°C for 2h and material to liquor ratio, 1:6. At this condition pulp yield was 42.4% with kappa number 10.3. KOH pulp bleached to 85% brightness by D0EpD1 bleaching sequences with ClO2 consumption of 25kg/ton of pulp. Silica and lignin were separated from the black liquor of KOH pulping. The amount of recovered silica, lignin and hemicelluloses were 10.4%, 8.4% and 13.0%. The papermaking properties of KOH pulp from rice straw were slightly better than those of corresponding NaOH pulp. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. High-yield pulping effluent treatment technologies

    International Nuclear Information System (INIS)

    Su, W.X.; Hsieh, J.S.

    1993-03-01

    The objective of this report is to examine the high-yield (mechanical) pulp processes with respect to environmental issues affected by the discharge of their waste streams. Various statistics are given that support the view that high-yield pulping processes will have major growth in the US regions where pulp mills are located, and sites for projects in the development phase are indicated. Conventional and innovative effluent-treatment technologies applicable to these processes are reviewed. The different types of mechanical pulping or high-yield processes are explained, and the chemical additives are discussed. The important relationship between pulp yield and measure of BOD in the effluent is graphically presented. Effluent contaminants are identified, along with other important characteristics of the streams. Current and proposed environmental limitations specifically related to mechanical pulp production are reviewed. Conventional and innovative effluent-treatment technologies are discussed, along with their principle applications, uses, advantages, and disadvantages. Sludge management and disposal techniques become an intimate part of the treatment of waste streams. The conclusion is made that conventional technologies can successfully treat effluent streams under current waste-water discharge limitations, but these systems may not be adequate when stricter standards are imposed. At present, the most important issue in the treatment of pulp-mill waste is the management and disposal of the resultant sludge

  13. Infection and Pulp Regeneration

    Directory of Open Access Journals (Sweden)

    Sahng G. Kim

    2016-03-01

    Full Text Available The regeneration of the pulp-dentin complex has been a great challenge to both scientists and clinicians. Previous work has shown that the presence of prior infection may influence the characteristics of tissues formed in the root canal space after regenerative endodontic treatment. The formation of ectopic tissues such as periodontal ligament, bone, and cementum has been observed in the root canal space of immature necrotic teeth with apical periodontitis, while the regeneration of dentin and pulp has been identified in previously non-infected teeth. The current regenerative endodontic therapy utilizes disinfection protocols, which heavily rely on chemical irrigation using conventional disinfectants. From a microbiological point of view, the current protocols may not allow a sufficiently clean root canal microenvironment, which is critical for dentin and pulp regeneration. In this article, the significance of root canal disinfection in regenerating the pulp-dentin complex, the limitations of the current regenerative endodontic disinfection protocols, and advanced disinfection techniques designed to reduce the microorganisms and biofilms in chronic infection are discussed.

  14. Hydrothermal carbonization of autoclaved municipal solid waste pulp and anaerobically treated pulp digestate

    Science.gov (United States)

    In this study, the autoclaved organic fraction of municipal solid waste pulp (OFMSW) and the digestate from OFMSW pulp after anaerobic digestion (AD) were processed by hydrothermal carbonization (HTC) at 200, 250, and 300 °C for 30 min and 2 h. The focus of this work was to evaluate the potential fo...

  15. Amino acid derivative-mediated detoxification and functionalization of dual cure dental restorative material for dental pulp cell mineralization.

    Science.gov (United States)

    Minamikawa, Hajime; Yamada, Masahiro; Iwasa, Fuminori; Ueno, Takeshi; Deyama, Yoshiaki; Suzuki, Kuniaki; Yawaka, Yasutaka; Ogawa, Takahiro

    2010-10-01

    Current dental restorative materials are only used to fill the defect of hard tissues, such as dentin and enamel, because of their cytotoxicity. Therefore, exposed dental pulp tissues in deep cavities must be first covered by a pulp capping material like calcium hydroxide to form a layer of mineralized tissue. However, this tissue mineralization is based on pathological reaction and triggers long-lasting inflammation, often causing clinical problems. This study tested the ability of N-acetyl cysteine (NAC), amino acid derivative, to reduce cytotoxicity and induce mineralized tissue conductivity in resin-modified glass ionomer (RMGI), a widely used dental restorative material having dual cure mechanism. Rat dental pulp cells were cultured on untreated or NAC-supplemented RMGI. NAC supplementation substantially increased the percentage of viable cells from 46.7 to 73.3% after 24-h incubation. Cell attachment, spreading, proliferative activity, and odontoblast-related gene and protein expressions increased significantly on NAC-supplemented RMGI. The mineralization capability of cells, which was nearly suppressed on untreated RMGI, was induced on NAC-supplemented RMGI. These improved behaviors and functions of dental pulp cells on NAC-supplemented RMGI were associated with a considerable reduction in the production of intracellular reactive oxygen species and with the increased level of intracellular glutathione reserves. These results demonstrated that NAC could detoxify and functionalize RMGIs via two different mechanisms involving in situ material detoxification and antioxidant cell protection. We believe that this study provides a new approach for developing dental restorative materials that enables mineralized tissue regeneration.

  16. EVALUATION OF CONDITION OF THE PULP BY PULSE OXIMETRY.

    Directory of Open Access Journals (Sweden)

    Dimitar Kosturkov

    2015-12-01

    Full Text Available Purpose: To conduct pulse oximetry (PO and electric pulp test (EPT on intact frontal teeth in clinically healthy patients aged between 18 and 25 years who do not have periodontal disease. Material/Methods: To achieve the aim 1058 teeth of 31 patients were studied. The following inclusion criteria for the study were set: 1. Age – 18-25 years. 2. Clinically healthy patient, who does not have any systemic diseases, do not take any medicine systematically. 3. Intact frontal teeth – without carious lesions, restorations or root canal treatment. 4. Lack of periodontal disease. Research was conducted with a pulse oximeter Contec™ - CMS60D and a custom made probe holder. Results: Average values obtained by pulse oximetry in upper jaw vary between 83% and 85%. In lower jaw – between 82% and 85%. 99% is the maximum and 48% is the minimum registered value. The average value of the measurement of all the teeth is 84%. The average saturation measured on the small finger of the right hand of the patient is 98%. The average value of EPT for all teeth is 4 μA. The maximum measured value is 20 μA, and the minimum - 1 μA. Conclusions: 1. Adequate study of the pulp includes two complementary methods – electric pulp test (evaluation of innervation and pulse oximetry (assessment of pulp microcirculation. 2. Teeth that are larger in size have larger values of PO and EPT, which is in direct relation to the size of their pulp chamber. 3. The total saturation, measured in the little finger of the right hand is greater than the one of the teeth.

  17. Pulping Variables, Storage Time and Pitch Deposit | Ogunwusi ...

    African Journals Online (AJOL)

    Pulp resin is also influenced by effective alkali concentration of the pulping medium. With increase in effective alkali concentration from 13% to 15%, pulp pitch is reduced. The interaction effect of storage and effective alkali concentration was not significant indicating that reduction in pulp pitch caused by effective alkali ...

  18. An ATLAS inner detector end-cap is placed in its cryostat

    CERN Multimedia

    2007-01-01

    The instrumentation housed inside the inner end-cap must be kept cool to avoid thermal noise. This cooling is achieved on ATLAS by placing the end-cap inside a liquid argon cryostat. The end-cap measures particles that are produced close to the direction of the beam pipe and would otherwise be missed.

  19. Dental pulp stem cells

    DEFF Research Database (Denmark)

    Ashri, N. Y.; Ajlan, S. A.; Aldahmash, Abdullah M.

    2015-01-01

    scaffold, and guided through signaling molecules. Dental pulp stem cells have been used in an increasing number of studies in dental tissue engineering. Those cells show mesenchymal (stromal) stem cell-like properties including self-renewal and multilineage differentiation potentials, aside from...... an updated review on dental pulp stem cells and their applications in periodontal regeneration, in combination with different scaffolds and growth factors....

  20. State-of-the-art synthetic membrane for capping landfills

    International Nuclear Information System (INIS)

    Kriofske, K.P.; Gagle, D.W.

    1991-01-01

    Very Low Density Polyethylene (VLDPE) has emerged as a superior capping material for landfill closures. Landfills must be capped by a material which will undergo substantial deformation in areas of localized settlement prior to rupture. Methane and hydrogen sulfide gases must be contained and directed to collection points without permeating the landfill cap. Vegetative growth in the cover sods will be protected by the gas impermeability of the geosynthetic membrane. VLDPE compounded with carbon black is minimally affected by radiation and is inert to ultraviolet rays. This property sustains VLDPE's ability to retard gas permeation at levels superior to other geosynthetics. Cover soil stability on long cap slopes in all weather conditions is crucial. It has been demonstrated in the laboratory and in full-scale, on-site test conditions that VLDPE exhibits friction characteristics equaling or exceeding other synthetics used for this purpose without diminishing physical and chemical properties. Large-scale, multiaxial stress tests have demonstrated the ability of VLDPE to deflect substantially in all directions of a potential settlement area. Only PVC can equal the elastic deformation properties of VLDPE, but PVC is more gas-permeable susceptible to degradation due to natural soil radiation or ultraviolet light and heat. Test results are presented to illustrate these points. The geosynthetic cap membrane must prevent water percolation into the landfill to prevent the formation of hazardous leachates. The use of a VLDPE cap reduces the depth of cap soils, thus increasing landfill volume. The economics and reduction in long-term liabilities of closure costs are enhanced by the use of VLDPE in the cap system. Since the expected half-life of polyethylene exceeds hundreds of years, the inclusion of VLDPE in the cap system will provide pollution security for many generations

  1. Nanofibrillated cellulose as paper additive in Eucalyptus pulps

    Directory of Open Access Journals (Sweden)

    Israel González

    2012-11-01

    Full Text Available In this work, the physical and mechanical properties of bleached Eucalyptus pulp reinforced with nanofibrillated cellulose (NFC are compared with those of traditional beaten pulp used in the making of writing/printing and offset printing papers. For this purpose, three different types of hardwood slurries were prepared: beaten pulps, unbeaten pulps reinforced with NFC, and slightly beaten pulps also reinforced with NFC. Physical and mechanical tests were performed on handsheets from these different slurries. The results showed that adding NFC to unbeaten pulps results in physical and mechanical properties similar to those in pulps used for printing/writing papers. Nevertheless, the best results were obtained in slurries previously beaten at slight conditions and subsequently reinforced with NFC. These results demonstrate that the addition of NFC allows a reduction in beating intensity without decreasing the desired mechanical properties for this specific purpose.

  2. A novel role for Twist-1 in pulp homeostasis.

    Science.gov (United States)

    Galler, K M; Yasue, A; Cavender, A C; Bialek, P; Karsenty, G; D'Souza, R N

    2007-10-01

    The molecular mechanisms that maintain the equilibrium of odontoblast progenitor cells in dental pulp are unknown. Here we tested whether homeostasis in dental pulp is modulated by Twist-1, a nuclear protein that partners with Runx2 during osteoblast differentiation. Our analysis of Twist-1(+/-) mice revealed phenotypic changes that involved an earlier onset of dentin matrix formation, increased alkaline phosphatase activity, and pulp stones within the pulp. RT-PCR analyses revealed Twist-1 expression in several adult organs, including pulp. Decreased levels of Twist-1 led to higher levels of type I collagen and Dspp gene expression in perivascular cells associated with the pulp stones. In mice heterozygous for both Twist-1 and Runx2 inactivation, the phenotype of pulp stones appeared completely rescued. These findings suggest that Twist-1 plays a key role in restraining odontoblast differentiation, thus maintaining homeostasis in dental pulp. Furthermore, Twist-1 functions in dental pulp are dependent on its interaction with Runx2.

  3. Evaluation of bleachability on pine and eucalyptus kraft pulps

    Directory of Open Access Journals (Sweden)

    Marcela Freitas Andrade

    2013-09-01

    Full Text Available In recent decades, the pulp industry has been changing and improving its manufacturing processes in order to enhance production capacity, product quality and environmental performance. The aim of this study was to evaluate the bleachability effect on the efficient washing and alkaline leaching in eucalyptus and pine Kraft pulps using three different bleaching sequences: AD(EPD, A/D(EPDP and DHT(EPDP. This study was carried out in two stages. In the first part, the optimum conditions for pulp bleaching in order to achieve a brightness of 90% ISO were established. The second step was a comparative study between the pulps that received alkaline leaching and efficient washing with reference pulp (without treatment. The brightness, viscosity, kappa number and HexA in pulp were analyzed. The three sequences studied reached the desired brightness, but the sequence AD(EPD produced a lower reagent consumption for the same brightness. In the three sequences studied, the efficient washing of the pulp after oxygen delignification has contributed significantly to the removal of dissolved organic and inorganic materials in the pulp and the alkaline leaching decreased significantly the pulp kappa number due to a higher pulp delignification and bleachability.

  4. Cervical Cap

    Science.gov (United States)

    ... giving birth vaginally, which means the cervical cap may not fit as well. Inconsistent or incorrect use of the cervical cap increases your risk of pregnancy. For example, you may get pregnant when using the cervical cap if: ...

  5. Association between dental pulp stones and calcifying nanoparticles.

    Science.gov (United States)

    Zeng, Jinfeng; Yang, Fang; Zhang, Wei; Gong, Qimei; Du, Yu; Ling, Junqi

    2011-01-07

    The etiology of dental pulp stones, one type of extraskeletal calcification disease, remains elusive to date. Calcifying nanoparticles (CNPs), formerly referred to as nanobacteria, were reported to be one etiological factor in a number of extraskeletal calcification diseases. We hypothesized that CNPs are involved in the calcification of the dental pulp tissue, and therefore investigated the link between CNPs and dental pulp stones. Sixty-five freshly collected dental pulp stones, each from a different patient, were analyzed. Thirteen of the pulp stones were examined for the existence of CNPs in situ by immunohistochemical staining (IHS), indirect immunofluorescence staining (IIFS), and transmission electron microscope (TEM). The remaining 52 pulp stones were used for isolation and cultivation of CNPs; the cultured CNPs were identified and confirmed via their shape and growth characteristics. Among the dental pulp stones examined in situ, 84.6% of the tissue samples staines positive for CNPs antigen by IHS; the corresponding rate by IIFS was 92.3 %. In 88.2% of the cultured samples, CNPs were isolated and cultivated successfully. The CNPs were visible under TEM as 200-400 nm diameter spherical particles surrounded by a compact crust. CNPs could be detected and isolated from a high percentage of dental pulp stones, suggesting that CNPs might play an important role in the calcification of dental pulp.

  6. Free toe pulp flap for finger pulp and volar defect reconstruction

    Directory of Open Access Journals (Sweden)

    Jyoshid R Balan

    2016-01-01

    Full Text Available Background: Fingertip injury requiring flap cover is very common in the modern era. The ideal cover should fulfill both functional and aesthetic improvement. Materials and Methods: From June 2015 to April 2016, we performed seven free toe pulp flaps for finger defect reconstruction. All patients were males. Five flaps were done in emergency post-traumatic cases, and two were done in elective set up. The cases included reconstruction of three thumbs, one index and one ring finger in an emergency set up and two ring fingers in the elective. Thumb reconstruction was done with great toe lateral pulp and the other digits reconstructed with second toe pulp flap. Follow-up evaluation included both functional and aesthetic assessment. Results: Five flaps survived completely, one suffered partial loss, and one flap failed completely. The median follow-up period was 9 months. The median duration of surgery was 255 min (range 210 to 300 min. The median two-point discrimination was 6.5 mm (range 4–8 mm. There was the return of temperature sensation in all patients; two had cold intolerance. The Semmes-Weinstein monofilament score varied from 3.61 to 5.07 (median filament index value 4.31/pressure value of 2 g/mm2. Three patients had delayed donor site wound healing. Conclusions: The free toe pulp flap is an efficient choice for fingertip and volar finger defects reconstruction with an excellent tissue match.

  7. Progress in LAr EndCap Calorimetry: News from the Hadronic EndCap Group.

    CERN Multimedia

    Oram, C.J.

    With module production and testing completed for the Hadronic EndCap calorimeter, the attention of the HEC group is heavily directed towards wheel assembly in building 180. Three of the four HEC wheels are now assembled and rotated, and work is progressing on assembling the final wheel. This year has been a busy year for the installation of components in the EndCap C cryostat: the signal feedthrough installation was completed April 22nd, the pre-sampler shortly thereafter and the Electro-Magnetic EndCap August 13th. This allowed the HEC group to start transferring the HEC wheels from the T6A storage cradle into the cryostat. The operation started in mid-September and has progressed, on or ahead of schedule, since then with the major milestones being: Insertion of 67 ton front HEC wheel October 3rd Insertion of 90 ton rear HEC wheel October 22nd. The wheel alignment has proved to be excellent, with the position of the centre of the front(rear) wheel with respect to the nominal position being displaced b...

  8. Pulping and paper properties of Palmyra palm fruit fibers

    Directory of Open Access Journals (Sweden)

    Waranyou Sridach

    2010-05-01

    Full Text Available Palmyra palm fruit fibers have the properties to be used as an alternative raw material of cellulosic pulps for papermaking.Acid and alkali pulping were investigated by using nitric acid and caustic soda on a laboratory scale, with the purpose of producing printing or writing grade pulp. The chemical composition of fiber strands from palmyra palm fruits were examined, such as holocellulose, cellulose, pentosan, lignin and extractives. The yields of acid and soda pulps were below 40%. The main physical and mechanical properties of hand sheets produced from acid and soda processes were evaluated on 80 g/m2 test sheets as functions of the following parameters: tensile index, tear index, and brightness. The mechanical properties of soda pulps were developed by twin-roll press while it was not necessary to fibrillate acidic pulps through the beating step. The soda pulp sheets presented a lower brightness than that of acidic pulp sheets. The mechanicaland physical properties of the acidic and alkaline pulps verified that they were of an acceptable quality for papermaking.

  9. Pemanfaatan Limbah Pulp Buah Semangka (Citrullus vulgaris, Schard) Untuk Pembuatan Nata De Watermelon Pulp Dengan Menggunakan Bakteri Acetobacter xylinum

    OpenAIRE

    Mawaddah

    2011-01-01

    This research done to know can or not the waste of watermelon pulp use to produce nata and how the effect of mass variation using to nata’s quality. This research was done with mass variation of watermelon pulp that is 10 g, 20 g, 30 g, 40 g, 50 g, 60 g, and watermelon pulp without adding sugar as control. Statistical analysis count the thickness, water content, ash content, fiber content and organoleptic test of texture, color, aroma, and taste of nata de watermelon pulp. The result show...

  10. Epigenetic regulation in dental pulp inflammation

    Science.gov (United States)

    Hui, T; Wang, C; Chen, D; Zheng, L; Huang, D; Ye, L

    2016-01-01

    Dental caries, trauma, and other possible factors could lead to injury of the dental pulp. Dental infection could result in immune and inflammatory responses mediated by molecular and cellular events and tissue breakdown. The inflammatory response of dental pulp could be regulated by genetic and epigenetic events. Epigenetic modifications play a fundamental role in gene expression. The epigenetic events might play critical roles in the inflammatory process of dental pulp injury. Major epigenetic events include methylation and acetylation of histones and regulatory factors, DNA methylation, and small non-coding RNAs. Infections and other environmental factors have profound effects on epigenetic modifications and trigger diseases. Despite growing evidences of literatures addressing the role of epigenetics in the field of medicine and biology, very little is known about the epigenetic pathways involved in dental pulp inflammation. This review summarized the current knowledge about epigenetic mechanisms during dental pulp inflammation. Progress in studies of epigenetic alterations during inflammatory response would provide opportunities for the development of efficient medications of epigenetic therapy for pulpitis. PMID:26901577

  11. PEMUTIHAN PULP DENGAN HIDROGEN PEROKSIDA

    Directory of Open Access Journals (Sweden)

    Ahmad M. Fuadi

    2012-01-01

    Full Text Available The use of bleaching agent has increased as the result of increasing of paper consumption. The conventional bleaching agent that commonly used is material containing of chlorine. This material is not environmentally friendly and should be replaced by environmentally benign chemical, such as H2O2. About 40 gram of dry Akasia pulp was mixed with 600 ml of distilled water was put into plastic bag heated in a water bath. When the temperature reached 630C, a solution of 4 % of H2O2 and distilled water was added to obtain 5 % consistency. This mixture was put into water bath and was heated for 2 hours. The same procedure was conducted with various concentration of H2O2, time and pH. At the end of the process, the pulp was dewatered and washed. The filtrate obtained from the initial dewatering was used to determine the residual of H2O2. The pulp was analyzed to determine brightness, fiber strength and kappa number. The maximum achievement of brightness was 62,1 % ISO, 6.86 of kappa number and 1.02 kg/15 mm of fiber strength, which are reached at16 % of the use of H2O2, pH 11 and 5 hours of bleaching time. This achievement is similar to bleaching result by the additional of 4% H2O2. Inefficient usage of H2O2 was caused by some metal ions in the pulp which facilitate the decomposition of H2O2 to produce oxygen and water which has not effect on increasing the brightness. To improve the bleach ability of H2O2, initial treatment to remove metal ions from pulp should be done. Seiring dengan meningkatnya kebutuhan kertas, kebutuhan bahan pemutih juga mengalami kenaikan. Saat ini bahan pemutih yang banyak digunakan adalah senyawa yang mengandung khlor. Senyawa ini sangat tidak ramah lingkungan, oleh karena itu, perlu dicari bahan yang ramah lingkungan untuk menggantikannya. Salah satunya adalah hidrogen peroksida. Pulp dari pohon akasia sebanyak 40 gram kering dicampur dengan 600 ml aquadest dimasukkan dalam kantung plastik dipanaskan dalam water bath

  12. Calcium Hydroxide-induced Proliferation, Migration, Osteogenic Differentiation, and Mineralization via the Mitogen-activated Protein Kinase Pathway in Human Dental Pulp Stem Cells.

    Science.gov (United States)

    Chen, Luoping; Zheng, Lisha; Jiang, Jingyi; Gui, Jinpeng; Zhang, Lingyu; Huang, Yan; Chen, Xiaofang; Ji, Jing; Fan, Yubo

    2016-09-01

    Calcium hydroxide has been extensively used as the gold standard for direct pulp capping in clinical dentistry. It induces proliferation, migration, and mineralization in dental pulp stem cells (DPSCs), but the underlying mechanisms are still unclear. The aim of this study was to investigate the role of the mitogen-activated protein (MAP) kinase pathway in calcium hydroxide-induced proliferation, migration, osteogenic differentiation, and mineralization in human DPSCs. Human DPSCs between passages 3 and 6 were used. DPSCs were preincubated with inhibitors of MAP kinases and cultured with calcium hydroxide. The phosphorylated MAP kinases were detected by Western blot analysis. Cell viability was analyzed via the methylthiazol tetrazolium assay. Cell migration was estimated using the wound healing assay. Alkaline phosphatase (ALP) expression was analyzed using the ALP staining assay. Mineralization was studied by alizarin red staining analysis. Calcium hydroxide significantly promoted the phosphorylation of the c-Jun N-terminal kinase (JNK), p38, and extracellular signal-regulated kinase. The inhibition of JNK and p38 signaling abolished calcium hydroxide-induced proliferation of DPSCs. The inhibition of JNK, p38, and extracellular signal-regulated kinase signaling suppressed the migration, ALP expression, and mineralization of DPSCs. Our study showed that the MAP kinase pathway was involved in calcium hydroxide-induced proliferation, migration, osteogenic differentiation, and mineralization in human DPSCs. Copyright © 2016 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  13. Basic effects of pulp refining on fiber properties--a review.

    Science.gov (United States)

    Gharehkhani, Samira; Sadeghinezhad, Emad; Kazi, Salim Newaz; Yarmand, Hooman; Badarudin, Ahmad; Safaei, Mohammad Reza; Zubir, Mohd Nashrul Mohd

    2015-01-22

    The requirement for high quality pulps which are widely used in paper industries has increased the demand for pulp refining (beating) process. Pulp refining is a promising approach to improve the pulp quality by changing the fiber characteristics. The diversity of research on the effect of refining on fiber properties which is due to the different pulp sources, pulp consistency and refining equipment has interested us to provide a review on the studies over the last decade. In this article, the influence of pulp refining on structural properties i.e., fibrillations, fine formation, fiber length, fiber curl, crystallinity and distribution of surface chemical compositions is reviewed. The effect of pulp refining on electrokinetic properties of fiber e.g., surface and total charges of pulps is discussed. In addition, an overview of different refining theories, refiners as well as some tests for assessing the pulp refining is presented. Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. Dental pulp stone formation during orthodontic treatment: A ...

    African Journals Online (AJOL)

    2015-06-21

    Jun 21, 2015 ... the presence of dental pulp stone, gender, age, tooth type and arches. Results: Dental pulp ... primary and permanent dentition.[1] Dental pulp stones .... interpretation provided training to familiarize the other observer with the ...

  15. Organo mercurials in pulp and paper industry

    Energy Technology Data Exchange (ETDEWEB)

    Bouveng, H O

    1967-01-01

    Today phenyl mercury acetate (PMA) is used in the paper and pulp industry for two purposes: slime control in paper machine systems and impregnation of wet mechanical pulp. PMA is a commonly used slimicide. It is used for slime control in such a way that a minor part (5-20% depending on mill operation) will reach the watercourse with the waste water and contaminate aquatic life. PMA used for impregnation concerns wet mechanical pulp produced for export as raw material, mostly for newsprint. Treatment of this pulp with PMA is necessary to avoid changes caused by molds and rot fungi.

  16. Isolation and Characterization of Human Dental Pulp Stem Cells from Cryopreserved Pulp Tissues Obtained from Teeth with Irreversible Pulpitis.

    Science.gov (United States)

    Malekfar, Azin; Valli, Kusum S; Kanafi, Mohammad Mahboob; Bhonde, Ramesh R

    2016-01-01

    Human dental pulp stem cells (DPSCs) are becoming an attractive target for therapeutic purposes because of their neural crest origin and propensity. Although DPSCs can be successfully cryopreserved, there are hardly any reports on cryopreservation of dental pulp tissues obtained from teeth diagnosed with symptomatic irreversible pulpitis during endodontic treatment and isolation and characterization of DPSCs from such cryopreserved pulp. The aim of this study was to cryopreserve the said pulp tissues to propagate and characterize isolated DPSCs. A medium consisting of 90% fetal bovine serum and 10% dimethyl sulfoxide was used for cryopreservation of pulp tissues. DPSCs were isolated from fresh and cryopreserved pulp tissues using an enzymatic method. Cell viability and proliferation were determined using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. DPSC migration and interaction were analyzed with the wound healing assay. Mesenchymal characteristics of DPSCs were verified by flow cytometric analysis of cell surface CD markers. The osteogenic and adipogenic potential of DPSCs was shown by von Kossa and oil red O staining methods, respectively, and the polymerase chain reaction method. We found no significant difference in CD marker expression and osteogenic and adipogenic differentiation potential of DPSCs obtained from fresh and cryopreserved dental pulp tissue. Our study shows that dental pulp can be successfully cryopreserved without losing normal characteristics and differentiation potential of their DPSCs, thus making them suitable for dental banking and future therapeutic purposes. Copyright © 2016 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  17. The relationship between pulp calcifications and salivary gland calcifications

    OpenAIRE

    Kaswan, Sumita; Patil, Santosh; Maheshwari, Sneha; Rahman, Farzan; Khandelwal, Suneet

    2014-01-01

    Aim: Pulp stones are discrete calcified bodies found in the dental pulp. Sialolithasis is the most common salivary gland disease. The aim of the present study was to determine the relationship between the pulp stones and salivary gland stones. Material and Methods: 196 patients were randomly selected from the out patient department for the study. The periapical radiographs for all patients were evaluated for the presence or absence of the narrowing of dental pulp chambers and pulp canals. The...

  18. Anti-pp,. cap alpha cap alpha. and p. cap alpha. elastic scattering at high energies and Chou-Yang conjecture

    Energy Technology Data Exchange (ETDEWEB)

    Saleem, M.; Fazal-e-Aleem; Rifique, M.

    1987-03-01

    The recent experimental measurements for anti-pp and ..cap alpha cap alpha.. elastic scattering at high energies have shown that the Chou-Yang conjecture regarding the relationship between the electromagnetic and the hadronic form factor of a particle is only an approximation. A new ansatz has been proposed to obtain hadronic form factors of proton and the ..cap alpha..-particle. These form factors have been used to explain the various characteristics of anti-pp, ..cap alpha cap alpha.. and p..cap alpha.. elastic scattering at high energies.

  19. Pulp and paper from blue agave waste from tequila production.

    Science.gov (United States)

    Idarraga, G; Ramos, J; Zuñiga, V; Sahin, T; Young, R A

    1999-10-01

    Pulping of blue agave waste, from the production of tequila, was evaluated by both chemical and biomechanical pulping processes. Two conventional and two organosolv systems were used to pulp the agave waste under a standard set of conditions. The soda-ethanol process was superior in terms of delignification and pulp properties in comparison to the soda and ethanol organosolv processes for pulping of agave waste; however, the kraft process gave the best strength properties. In general, the strength of the agave waste pulps was rather poor in comparison to wood and other agro-based pulps; however, the tear strength was relatively high. This result is typical of poorly bonded sheets and may be due to the coarseness of the agave fibers and/or loss of hemicelluloses in the steaming process for the tequila production. Fungal treatment of the agave waste with Ceriporiopsis subvermispora reduced the energy consumption for mechanical refining but gave biomechanical pulps with inferior strength properties. The blue agave chemical pulps should be suitable for blending with softwood kraft pulps for publication grade paper.

  20. CAP DIRECT PAYMENTS AS THE MAIN TOOL TO SUPPORT THE EU FARMERS: AN ANALYSIS OF THEIR IMPLEMENTATION BASED ON SPAIN EXPERIENCE

    Directory of Open Access Journals (Sweden)

    Liliana CIMPOIES

    2016-01-01

    Full Text Available Direct payments are the main tool of the EU Common Agricultural Policy (CAP and the main objective is to support EU farmers incomes. They benefit from a particular attention during the new 2014-2020 reform. For the first time direct payments were introduced in 1992 after MacSharry reform and known as coupled payments (payments per hectare and animal head. In 2003 were introduced the decoupled payments focused at encouraging farmers and enhancing the competitiveness and sustainability of the EU agriculture. The aim of this paper is to analyze the application of direct payments as main support instrument to the EU farmers based on Spain experience. In Spain the new changes in the Single Payment Scheme (SPS were first introduced in 2006, beginning with the regime of partial decoupling, until 2012, when the coupled payments disappeared, being integrated under the SPS or transformed into additional payment. In this analysis, secondary data provided by the Spanish Agrarian Guarantee Fund (FEGA, Ministry of Agriculture, Food and Environment of Spain (MAGRAMA and Farm Accountancy Data Network (FADN. The analyzed data refers to the amount of allocated direct payments in Spain and EU countries, number of beneficiaries and its distribution on territorial aspect. So far, the distribution of direct payments in Spain, similar to other EU member states, is unequal, as a result of various factors, as the CAP development and diversity of production, the use of historical references to fix the decoupled payments per farm and others.

  1. Effects of some extrusion variables on physicochemical characteristics of extruded corn starch-passion fruit pulp (Passiflora edulis) snacks.

    Science.gov (United States)

    Cortés, R Nallely Falfán; Guzmán, Iñigo Verdalet; Martínez-Bustos, Fernando

    2014-12-01

    The aim of this work was to study the effect of the addition of passion fruit pulp (PFP: 0-7%), the variation of barrel temperature in the third zone extruder (BT: 80-140 °C) and feed moisture (FM:16-30%) in a blend of corn starch and passion fruit pulp on different physicochemical characteristics of directly expanded snacks by extrusion technology. Single-screw laboratory extruder and a central, composite, rotatable experimental design were used. Expansion index of extrudates ranged between 1.0 and 1.8. Decreasing of feed moisture (18%), passion fruit pulp concentration (1.42%) and the increasing of barrel temperature (127 °C) resulted in higher expansion index. The increasing of feed moisture and passion fruit pulp concentration resulted in higher penetration force values of extrudates. The passion fruit pulp concentration showed a highly significant effect (p ≤ 0.01) on the L *, a * and b * parameters. Passion fruit pulp has a reasonable source of β-carotene, proteins and dietary fibers that can be added to expanded snacks.

  2. Effects of transportation time after extraction on the magnetic cryopreservation of pulp cells of rat dental pulp

    Directory of Open Access Journals (Sweden)

    Mao-Suan Huang

    2011-03-01

    Conclusions: The freezing technique used in this animal study provided positive effects on pulp cell storage. In addition, the storage time before the freezing procedure is an important issue for cryopreserving pulp cells in intact teeth.

  3. Final Technical Report Steam Cycle Washer for Unbleached Pulp

    Energy Technology Data Exchange (ETDEWEB)

    Starkey, Yvonne; Salminen, Reijo; Karlsnes, Andy

    2008-09-22

    Project Abstract for “Steam Cycle Washer for Unbleached Pulp” When completed, the patented SC Washer will provide an innovative, energy efficient demonstration project to wash unbleached pulp using a pressure vessel charged with steam. The Port Townsend Paper Corporation’s pulp mill in Port Townsend, WA was initially selected as the host site for conducting the demonstration of the SCW. Due to 2006 and 2007 delays in the project caused by issues with 21st Century Pulp & Paper, the developer of the SCW, and the 2007 bankruptcy proceedings and subsequent restructuring at Port Townsend Paper, the mill can no longer serve as a host site. An alternate host site is now being sought to complete the commercial demonstration of the Steam Cycle Washer for Unbleached Pulp. Additionally, estimated costs to complete the project have more than doubled since the initial estimates for the project were completed in 2002. Additional grant funding from DOE was sought and in July, 2008 the additional DOE funds were procured under a new DOE award, DE-PS36-08GO98014 issued to INL. Once the new host site is secured the completion of the project will begin under the management of INL. Future progress reports and milestone tracking will be completed under requirements of new DOE Award Number DE-PS36-08GO98014. The following are excerpts from the project Peer Review completed in 2006. They describe the project in some detail. Additional information can be found by reviewing DOE Award Number: DE-PS36-08GO98014. 5. Statement of Problem and Technical Barriers: The chemical pulping industry is one of the major users of fresh water in the United States. On average the industry uses over 80 tons of water to produce one ton of pulp, some states use up to 50% more (Washington 120 and Wisconsin 140). In order to process one ton of pulp using 80 tons of process water, a large amount of: • energy is used in process heat and • power is required for pumping the large volume of pulp slurries

  4. Predictive values of thermal and electrical dental pulp tests: a clinical study.

    Science.gov (United States)

    Villa-Chávez, Carlos E; Patiño-Marín, Nuria; Loyola-Rodríguez, Juan P; Zavala-Alonso, Norma V; Martínez-Castañón, Gabriel A; Medina-Solís, Carlo E

    2013-08-01

    For a diagnostic test to be useful, it is necessary to determine the probability that the test will provide the correct diagnosis. Therefore, it is necessary to calculate the predictive value of diagnostics. The aim of the present study was to identify the sensitivity, specificity, positive and negative predictive values, accuracy, and reproducibility of thermal and electrical tests of pulp sensitivity. The thermal tests studied were the 1, 1, 1, 2-tetrafluoroethane (cold) and hot gutta-percha (hot) tests. For the electrical test, the Analytic Technology Pulp Tester (Analytic Technology, Redmond, WA) was used. A total of 110 teeth were tested: 60 teeth with vital pulp and 50 teeth with necrotic pulps (disease prevalence of 45%). The ideal standard was established by direct pulp inspection. The sensitivities of the diagnostic tests were 0.88 for the cold test, 0.86 for the heat test, and 0.76 for the electrical test, and the specificity was 1.0 for all 3 tests. The negative predictive value was 0.90 for the cold test, 0.89 for the heat test, and 0.83 for the electrical test, and the positive predictive value was 1.0 for all 3 tests. The highest accuracy (0.94) and reproducibility (0.88) were observed for the cold test. The cold test was the most accurate method for diagnostic testing. Copyright © 2013 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  5. Influence of Soda Pulping Variables on Properties of Pineapple (Ananas comosus Merr. Leaf Pulp and Paper Studied by Face-Centered Composite Experimental Design

    Directory of Open Access Journals (Sweden)

    Jantharat Wutisatwongkul

    2016-01-01

    Full Text Available Face-centered composite design (FCC was used to study the effect of pulping variables: soda concentration (4-5 wt%, temperature (90–130°C, and pulping time (20–60 min on the properties of pineapple leaf pulp and paper employing soda pulping. Studied pulp responses were screened yield and lignin content (kappa number. Paper properties, which include tensile index, burst index, and tear index, were also investigated. Effects of the pulping variables on the properties were statistically analyzed using Minitab 16. The optimum conditions to obtain the maximum tensile index were soda concentration of 4 wt%, pulping temperature of 105°C, and pulping time of 20 min. The predicted optimum conditions provided tensile index, burst index, tear index, screened yield, and kappa number of 44.13 kN·m/kg, 1.76 kPa·m2, 1.68 N·m2/kg, 21.29 wt%, and 28.12, respectively, and were experimentally confirmed.

  6. Directional genetic selection by pulp mill effluent on multiple natural populations of three-spined stickleback (Gasterosteus aculeatus).

    Science.gov (United States)

    Lind, Emma E; Grahn, Mats

    2011-05-01

    Contamination can cause a rapid environmental change which may require populations to respond with evolutionary changes. To evaluate the effects of pulp mill effluents on population genetics, we sampled three-spined sticklebacks (Gasterosteus aculeatus) near four pulp mills and four adjacent reference sites and analyzed Amplified Fragment Length Polymorphism (AFLP) to compare genetic variability. A fine scale genetic structure was detected and samples from polluted sites separated from reference sites in multidimensional scaling plots (Pselection. When removing 13 F(ST)-outlier loci, significant at the Pselective agent on natural populations of G. aculeatus, causing a convergence in genotype composition change at multiple sites in an open environment. © The Author(s) 2011. This article is published with open access at Springerlink.com

  7. Residual liquors of the pulp industries. 1. Lignohemicelluloses and silica from soda black liquors used as adjuvants in the manufacture of compact paper board

    Energy Technology Data Exchange (ETDEWEB)

    El-Saied, H; Nada, A A.M.; Ibrahem, A A

    1982-01-01

    Lignohemicellulosic materials and SiO/sub 2/ were recovered from black liquors from the pulping of rice straw. The lignohemicellulose improved the properties of compact cardboard from cotton stalk pulp more effectively than those of cardboard from rice straw pulp, while the effectiveness of the SiO/sub 2/ was in the opposite direction. The water resistance of cardboard from cotton stalk pulp made it suitable for both outdoor and indoor use, while cardboard from rice straw pulp could be used only indoors. The lignohemicellulose and SiO/sub 2/ could effectively replace more expensive phenolic resins in cardboard manufacture.

  8. Water requirements of the pulp and paper industry

    Science.gov (United States)

    Mussey, Orville D.

    1955-01-01

    Water, of varied qualities, is used for several purposes in the manufacture of pulp and paper, as a vehicle for transporting the constituents of paper in the paper machines; as process water for cooking wood chips to make pulp; as a medium for heat transfer; and for washing the pulpwood, the woodpulp, and the machines that handle the pulp. About 3,200 million gallons of water was withdrawn from surface- and ground-water sources each day during 1950 for the use of the pulp and paper industry. This is about 4 percent of the total estimated industrial withdrawal of water in the Nation The paper industry in the United States has been growing at a rapid rate. It has increased about tenfold in the last 50 years and has doubled every 15 years. The 1950 production of paper was about 24 million tons, which amounts to about 85 percent of the domestic consumption. In 1950, the pulp mills of the country produced more than 14 million tons of woodpulp, which supplied about 85 percent of the demand by the paper mills and other industries. The remainder of the fiber for paper manufacture was obtained from imported woodpulp, from reclaimed wastepaper, and from other fibers including rags and straw. The nationwide paper consumption for 1955 has been estimated at 31,700,000 tons. Woodpulp is classified according to the process by which it is made. Every woodpulp has characteristics that are carried over into the many and diverse grades of paper. Groundwood pulp is manufactured by simply grinding up wood and refining the resulting product. Soda, sulfite, and sulfate pulps are manufactured by chemically breaking down the lignin that cements the cellulose of the wood together and removing, cleaning, and sometimes bleaching the resulting fibers. Some woodpulp is produced by other methods. Sulfate-pulp mills are increasing in number and in rated daily capacity and are manufacturing more than half of the present domestic production of woodpulp. Most of the newer and larger woodpulp mills

  9. Influence of Heartwood on Wood Density and Pulp Properties Explained by Machine Learning Techniques

    Directory of Open Access Journals (Sweden)

    Carla Iglesias

    2017-01-01

    Full Text Available The aim of this work is to develop a tool to predict some pulp properties e.g., pulp yield, Kappa number, ISO brightness (ISO 2470:2008, fiber length and fiber width, using the sapwood and heartwood proportion in the raw-material. For this purpose, Acacia melanoxylon trees were collected from four sites in Portugal. Percentage of sapwood and heartwood, area and the stem eccentricity (in N-S and E-W directions were measured on transversal stem sections of A. melanoxylon R. Br. The relative position of the samples with respect to the total tree height was also considered as an input variable. Different configurations were tested until the maximum correlation coefficient was achieved. A classical mathematical technique (multiple linear regression and machine learning methods (classification and regression trees, multi-layer perceptron and support vector machines were tested. Classification and regression trees (CART was the most accurate model for the prediction of pulp ISO brightness (R = 0.85. The other parameters could be predicted with fair results (R = 0.64–0.75 by CART. Hence, the proportion of heartwood and sapwood is a relevant parameter for pulping and pulp properties, and should be taken as a quality trait when assessing a pulpwood resource.

  10. Performance of Schizolobium amazonicum Wood in Bleached Kraft Pulp Production

    Directory of Open Access Journals (Sweden)

    Camila Sarto

    2015-05-01

    Full Text Available This study aimed to evaluate the characteristics of Schizolobium amazonicum wood, specifically its performance in bleached kraft pulp production and the characteristics of its pulp. Chips of Schizolobium amazonicum and Eucalyptus grandis x Eucalyptus urophylla (reference were used. The following parameters were evaluated in the wood: basic density, total extractives, total lignin, holocellulose, and fiber morphology. The pulping simulations were carried out in a laboratory digester, with parameters set to obtain pulp with kappa number 19 ± 0.5. Both pulps were bleached in a PFI mill and submitted to physical-mechanical tests. The results showed that S. amazonicum wood has low basic density and higher content of extractives when compared to E. grandis x E. urophylla wood. For pulping, S. amazonicum required higher alkali charge and H factor to achieve the same delignification level of E. grandis x E. urophylla, resulting in a lower yield, pulp with lower viscosity, and higher wood specific consumption. During bleaching, the brightness gain and final viscosity of S. amazonicum pulp were lower than E. grandis x E. urophylla pulp. Moreover, S. amazonicum pulp had worse physical-mechanical characteristics than E. grandis x E. urophylla.

  11. Material properties of CorCap passive cardiac support device.

    Science.gov (United States)

    Chitsaz, Sam; Wenk, Jonathan F; Ge, Liang; Wisneski, Andrew; Mookhoek, Aart; Ratcliffe, Mark B; Guccione, Julius M; Tseng, Elaine E

    2013-01-01

    Myocardial function deteriorates during ventricular remodeling in patients with congestive heart failure (HF). Ventricular restraint therapy using a cardiac support device (CSD) is designed to reduce the amount of stress inside the dilated ventricles, which in turn halts remodeling. However, as an open mesh surrounding the heart, it is unknown what the mechanical properties of the CSD are in different fiber orientations. Composite specimens of CorCap (Acorn Cardiovascular, Inc, St. Paul, MN) CSD fabric and silicone were constructed in different fiber orientations and tested on a custom-built biaxial stretcher. Silicone controls were made and stretched to detect the parameters of the matrix. CSD coefficients were calculated using the composite and silicone matrix stress-strain data. Stiffness in different fiber orientations was determined. Silicone specimens exerted a linear behavior, with stiffness of 2.57 MPa. For the composites with 1 fiber set aligned with respect to the stretch axes, stiffness in the direction of the aligned fiber set was higher than that in the cross-fiber direction (14.39 MPa versus 5.66 MPa), indicating greater compliance in the cross-fiber direction. When the orientation of the fiber sets in the composite were matched to the expected clinical orientation of the implanted CorCap, the stiffness in the circumferential axis (with respect to the heart) was greater than in the longitudinal axis (10.55 MPa versus 9.70 MPa). The mechanical properties of the CorCap demonstrate directionality with greater stiffness circumferentially than longitudinally. Implantation of the CorCap clinically should take into account the directionality of the biomechanics to optimize ventricular restraint. Copyright © 2013 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.

  12. 78 FR 31315 - Kraft Pulp Mills NSPS Review

    Science.gov (United States)

    2013-05-23

    ... Kraft Pulp Mills NSPS Review; Proposed Rule #0;#0;Federal Register / Vol. 78 , No. 100 / Thursday, May...-OAR-2012-0640; FRL-9815-9] RIN 2060-AR64 Kraft Pulp Mills NSPS Review AGENCY: Environmental Protection... performance standards for kraft pulp mills. These revised standards include particulate matter emission limits...

  13. Cradle Cap (For Parents)

    Science.gov (United States)

    ... Safe Videos for Educators Search English Español Cradle Cap (Infantile Seborrheic Dermatitis) KidsHealth / For Parents / Cradle Cap ( ... many babies develop called cradle cap. About Cradle Cap Cradle cap is the common term for seborrheic ...

  14. Improved pulp bleaching potential of Bacillus subtilis WB800 through overexpression of three lignolytic enzymes from various bacteria.

    Science.gov (United States)

    Ozer, Aysegul; Uzuner, Ugur; Guler, Halil Ibrahim; Ay Sal, Fulya; Belduz, Ali Osman; Deniz, Ilhan; Canakci, Sabriye

    2017-12-29

    A chemical bleaching process of paper pulps gives off excessive amount of chlorinated organic wastes mostly released to environment without exposing complete bioremediaton. Recent alternative and eco-friendly approaches toward pulp bleaching appear more responsive to environmental awareness. Here we report, direct use of a recombinant Bacillus subtilis bacterium for pulp bleaching, endowed with three ligninolytic enzymes from various bacteria. In addition, efficient bleaching performance from glutathione-S-transferase (GST) biocatalyst tested for the first time in pulp bleaching applications was also achieved. Simultaneous and extracellular overproduction of highly active GST, laccase, and lignin peroxidase catalysts were also performed by Bacillus cells. Both enhanced bleaching success and improved delignification rates were identified when enzyme combinations tested on both pine kraft and waste paper pulps, ranging from 69.75% to 79.18% and 60.89% to 74.65%, respectively. Furthermore, when triple enzyme combination applied onto the papers from pine kraft and waste pulps, the best ISO brightness values were identified as 66.45% and 64.67%, respectively. The delignification rates of pulp fibers exposed to various enzymatic bleaching sequences were comparatively examined under SEM. In conclusion, the current study points out that in near future, a more fined-tuned engineering of pulp-colonizing bacteria may become a cost-effective and environmentally friendly alternative to chemical bleaching. © 2017 International Union of Biochemistry and Molecular Biology, Inc.

  15. Sisal organosolv pulp as reinforcement for cement based composites

    Directory of Open Access Journals (Sweden)

    Ana Paula Joaquim

    2009-09-01

    Full Text Available The present work describes non-conventional sisal (Agave sisalana chemical (organosolv pulp from residues of cordage as reinforcement to cement based materials. Sisal organosolv pulp was produced in a 1:1 ethanol/water mixture and post chemically and physically characterized in order to compare its properties with sisal kraft pulp. Cement based composites reinforced with organosolv or kraft pulps and combined with polypropylene (PP fibres were produced by the slurry de-watering and pressing method as a crude simulation of the Hatschek process. Composites were evaluated at 28 days of age, after exposition to accelerated carbonation and after 100 soak/dry cycles. Composites containing organosolv pulp presented lower mechanical strength, water absorption and apparent porosity than composites reinforced with kraft pulp. The best mechanical performance after ageing was also achieved by samples reinforced with kraft pulp. The addition of PP fibres favoured the maintenance of toughness after ageing. Accelerated carbonation promoted the densification of the composites reinforced with sisal organosolv + PP fibres.

  16. Characterization and Degradation of Pectic Polysaccharides in Cocoa Pulp.

    Science.gov (United States)

    Meersman, Esther; Struyf, Nore; Kyomugasho, Clare; Jamsazzadeh Kermani, Zahra; Santiago, Jihan Santanina; Baert, Eline; Hemdane, Sami; Vrancken, Gino; Verstrepen, Kevin J; Courtin, Christophe M; Hendrickx, Marc; Steensels, Jan

    2017-11-08

    Microbial fermentation of the viscous pulp surrounding cocoa beans is a crucial step in chocolate production. During this process, the pulp is degraded, after which the beans are dried and shipped to factories for further processing. Despite its central role in chocolate production, pulp degradation, which is assumed to be a result of pectin breakdown, has not been thoroughly investigated. Therefore, this study provides a comprehensive physicochemical analysis of cocoa pulp, focusing on pectic polysaccharides, and the factors influencing its degradation. Detailed analysis reveals that pectin in cocoa pulp largely consists of weakly bound substances, and that both temperature and enzyme activity play a role in its degradation. Furthermore, this study shows that pulp degradation by an indigenous yeast fully relies on the presence of a single gene (PGU1), encoding for an endopolygalacturonase. Apart from their basic scientific value, these new insights could propel the selection of microbial starter cultures for more efficient pulp degradation.

  17. Dynamics of the quiet polar cap

    International Nuclear Information System (INIS)

    Carlson, H.C. Jr.

    1990-01-01

    Work in the past has established that a few percent of the time, under northward interplanetary magnetic field and thus magnetically quiet conditions, sun aligned arcs are found in the polar cap with intensities greater than the order of a kilo Rayleigh in the visible. Here we extend this view. We first note that imaging systems with sensitivity down to tens of Rayleighs in the visible find sun aligned arcs in the polar cap far more often, closer to half the time than a few percent. Furthermore, these sun aligned arcs have simple electrodynamics. They mark boundaries between rapid antisunward flow of ionospheric plasma on their dawn side and significantly slower flow, or even sunward flow, on their dusk side. Since the sun aligned arcs are typically the order of 1000 km to transpolar in the sun-earth direction, and the order of 100 km or less in the dawn-dusk direction, they demarcate lines of strongly anisotropic ionospheric flow shears or convection cells. The very quiet polar cap (strongly northward IMF) is in fact characterized by the presence of sun aligned arcs and multiple highly anisotropic ionospheric flow shears. Sensitive optical images are a valuable diagnostic with which to study polar ionospheric convection under these poorly understood conditions. (author)

  18. Activated carbon prepared from coffee pulp: potential adsorbent of organic contaminants in aqueous solution.

    Science.gov (United States)

    Gonçalves, Maraisa; Guerreiro, Mário César; Ramos, Paulize Honorato; de Oliveira, Luiz Carlos Alves; Sapag, Karim

    2013-01-01

    The processing of coffee beans generates large amounts of solid and liquid residues. The solid residues (pulp, husk and parchment) represent a serious environmental problem and do not have an adequate disposal mechanism. In this work, activated carbons (ACs) for adsorption of organic compounds were prepared from coffee pulp by controlled temperature at different pulp/Na2HPO4 ratios (4:1, 2:1, 5:4 and 1:1). The N2 adsorption/desorption isotherms showed ACs with high quantities of mesopores and micropores and specific surface areas of 140, 150, 450 and 440 m(2)g(-1) for AC 4:1, AC 2:1, AC 5:4 and AC 1:1, respectively. The prepared material AC 5:4 showed a higher removal capacity of the organic contaminants methylene blue (MB), direct red (DR) and phenol than did a Merck AC. The maximum capacities for this AC are approximately 150, 120 and 120 mg g(-1) for MB, DR and phenol, respectively. Thus, a good adsorbent was obtained from coffee pulp, an abundant Brazilian residue.

  19. Cell-derived micro-environment helps dental pulp stem cells promote dental pulp regeneration.

    Science.gov (United States)

    Zhang, Xuexin; Li, Hui; Sun, Jingjing; Luo, Xiangyou; Yang, Hefeng; Xie, Li; Yang, Bo; Guo, Weihua; Tian, Weidong

    2017-10-01

    The function of the dental pulp is closely connected to the extracellular matrix (ECM) structure, and ECM has received significant attention due to its biological functions for regulating cells. As such, the interaction between the ECM niche and cells is worth exploring for potential clinical uses. In this study, dental pulp stem cell (DPSC)-derived ECM (DPM) was prepared through cell culture and decellularization to function as the cell niche, and changes in DPSC behaviour and histological analysis of dental pulp tissue regeneration were evaluated following the DPM culture. DPM promoted the replication of DPSCs and exhibited retention of their mineralization. Then, the DPM-based culture strategy under odontogenic culture medium was further investigated, and the mineralization-related markers showed that DPSCs were regulated towards odontogenic differentiation. Dental pulp-like tissue with well-arranged ECM was harvested after a 2-month subcutaneous implantation in nude mice with DPM application. Additionally, DPSCs cultured on the plastic culture surface showed the up-regulation of mineralization makers in vitro, but there was a disorder in matrix formation and mineralization when the cells were cultured in vivo. DPM-based cultivation could serve as a cell niche and modulate DPSC behaviour, and this method also provided an alternative to harvest tissue-specific ECM and provided a strategy for ECM-cell interaction. © 2017 John Wiley & Sons Ltd.

  20. Integrated Bioethanol Fermentation/Anaerobic Digestion for Valorization of Sugar Beet Pulp

    Directory of Open Access Journals (Sweden)

    Joanna Berlowska

    2017-08-01

    Full Text Available Large amounts of waste biomass are generated in sugar factories from the processing of sugar beets. After diffusion with hot water to draw the sugar from the beet pieces, a wet material remains called pulp. In this study, waste sugar beet pulp biomass was enzymatically depolymerized, and the obtained hydrolyzates were subjected to fermentation processes. Bioethanol, biomethane, and biohydrogen were produced directly from the substrate or in combined mode. Stillage, a distillery by-product, was used as a feedstock for anaerobic digestion. During biosynthesis of ethanol, most of the carbohydrates released from the sugar beet pulp were utilized by a co-culture of Saccharomyces cerevisiae Ethanol Red, and Scheffersomyces stipitis LOCK0047 giving 12.6 g/L of ethanol. Stillage containing unfermented sugars (mainly arabinose, galactose and raffinose was found to be a good substrate for methane production (444 dm3 CH4/kg volatile solids (VS. Better results were achieved with this medium than with enzymatic saccharified biomass. Thermal pre-treatment and adjusting the pH of the inoculum resulted in higher hydrogen production. The largest (p < 0.05 hydrogen yield (252 dm3 H2/kg VS was achieved with sugar beet stillage (SBS. In contrast, without pre-treatment the same medium yielded 35 dm3 H2/kg VS. However, dark fermentation of biohydrogen was more efficient when sugar beet pulp hydrolyzate was used.

  1. Kinetics of pulp mill effluent treatment by ozone-based processes

    International Nuclear Information System (INIS)

    Ko, Chun-Han; Hsieh, Po-Hung; Chang, Meng-Wen; Chern, Jia-Ming; Chiang, Shih-Min; Tzeng, Chewn-Jeng

    2009-01-01

    The wastewaters generated from wood pulping and paper production processes are traditionally treated by biological and physicochemical processes. In order to reduce chemical oxygen demand (COD) and color to meet increasingly strict discharge standards, advanced oxidation processes (AOPs) are being adapted as polishing treatment units. Various ozone-based processes were used in this study to treat simulated wastewaters prepared from black liquor from a hardwood Kraft pulp mill in Taiwan. The experimental results showed that the COD and color were primarily removed by direct ozone oxidation and activated carbon adsorption. While the addition of activated carbon could enhance the COD and color removal during ozonation, the addition of hydrogen peroxide improved the color removal only. For the various ozone-based treatment processes, kinetic models were developed to satisfactorily predict the COD and color removal rates. According to the kinetic parameters obtained from the various ozone-based processes, the enhanced COD and color removal of ozonation in the presence of activated carbon was attributed to the regeneration of the activated carbon by ozonation. These kinetic models can be used for reactor design and process design to treat pulping wastewater using ozone-based processes.

  2. Biosynthesis of gold nanoparticles using Capsicum annuum var. grossum pulp extract and its catalytic activity

    Science.gov (United States)

    Yuan, Chun-Gang; Huo, Can; Yu, Shuixin; Gui, Bing

    2017-01-01

    Biological synthesis approach has been regarded as a green, eco-friendly and cost effective method for nanoparticles preparation without any toxic solvents and hazardous bi-products during the process. This present study reported a facile and rapid biosynthesis method for gold nanoparticles (GNPs) from Capsicum annuum var. grossum pulp extract in a single-pot process. The aqueous pulp extract was used as biotic reducing agent for gold nanoparticle growing. Various shapes (triangle, hexagonal, and quasi-spherical shapes) were observed within range of 6-37 nm. The UV-Vis spectra showed surface plasmon resonance (SPR) peak for the formed GNPs at 560 nm after 10 min incubation at room temperature. The possible influences of extract amount, gold ion concentration, incubation time, reaction temperature and solution pH were evaluated to obtain the optimized synthesis conditions. The effects of the experimental factors on NPs synthesis process were also discussed. The produced gold nanoparticles were characterized by transform electron microscopy (TEM), X-ray diffraction (XRD), energy dispersive X-ray (EDS) and Fourier Transform infrared spectroscopy (FTIR). The results demonstrated that the as-obtained GNPs were well dispersed and stable with good catalytic activity. Biomolecules in the aqueous extract were responsible for the capping and stabilization of GNPs.

  3. Temperature Distribution within a Cold Cap during Nuclear Waste Vitrification.

    Science.gov (United States)

    Dixon, Derek R; Schweiger, Michael J; Riley, Brian J; Pokorny, Richard; Hrma, Pavel

    2015-07-21

    The kinetics of the feed-to-glass conversion affects the waste vitrification rate in an electric glass melter. The primary area of interest in this conversion process is the cold cap, a layer of reacting feed on top of the molten glass. The work presented here provides an experimental determination of the temperature distribution within the cold cap. Because direct measurement of the temperature field within the cold cap is impracticable, an indirect method was developed in which the textural features in a laboratory-made cold cap with a simulated high-level waste feed were mapped as a function of position using optical microscopy, scanning electron microscopy, energy dispersive spectroscopy, and X-ray diffraction. The temperature distribution within the cold cap was established by correlating microstructures of cold-cap regions with heat-treated feed samples of nearly identical structures at known temperatures. This temperature profile was compared with a mathematically simulated profile generated by a cold-cap model that has been developed to assess the rate of glass production in a melter.

  4. Direct colorimetric detection of unamplified pathogen DNA by dextrin-capped gold nanoparticles.

    Science.gov (United States)

    Baetsen-Young, Amy M; Vasher, Matthew; Matta, Leann L; Colgan, Phil; Alocilja, Evangelyn C; Day, Brad

    2018-03-15

    The interaction between gold nanoparticles (AuNPs) and nucleic acids has facilitated a variety of diagnostic applications, with further diversification of synthesis match bio-applications while reducing biotoxicity. However, DNA interactions with unique surface capping agents have not been fully defined. Using dextrin-capped AuNPs (d-AuNPs), we have developed a novel unamplified genomic DNA (gDNA) nanosensor, exploiting dispersion and aggregation characteristics of d-AuNPs, in the presence of gDNA, for sequence-specific detection. We demonstrate that d-AuNPs are stable in a five-fold greater salt concentration than citrate-capped AuNPs and the d-AuNPs were stabilized by single stranded DNA probe (ssDNAp). However, in the elevated salt concentrations of the DNA detection assay, the target reactions were surprisingly further stabilized by the formation of a ssDNAp-target gDNA complex. The results presented herein lead us to propose a mechanism whereby genomic ssDNA secondary structure formation during ssDNAp-to-target gDNA binding enables d-AuNP stabilization in elevated ionic environments. Using the assay described herein, we were successful in detecting as little as 2.94 fM of pathogen DNA, and using crude extractions of a pathogen matrix, as few as 18 spores/µL. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. 75 FR 82335 - Airworthiness Directives; APEX Aircraft Model CAP 10 Airplanes

    Science.gov (United States)

    2010-12-30

    ... accident occurred to a CAP 10C, in which the pilot lost control of the aeroplane. The following investigation has revealed that the probable cause of the accident was the improper locking of a turnbuckle... specifically invite comments on the overall regulatory, economic, environmental, and energy aspects of this...

  6. Treatment of deep caries lesions in adults: randomized clinical trials comparing stepwise vs. direct complete excavation, and direct pulp capping vs. partial pulpotomy

    DEFF Research Database (Denmark)

    Bjørndal, Lars; Reit, Claes; Bruun, Gitte Hoffmann

    2010-01-01

    Less invasive excavation methods have been suggested for deep caries lesions. We tested the effects of stepwise vs. direct complete excavation, 1 yr after the procedure had been carried out, in 314 adults (from six centres) who had received treatment of a tooth with deep caries. The teeth had car...

  7. Nutrient contents of the fresh pulps and dried pulp cakes of vitellaria paradoxa of Gulu District, Uganda

    DEFF Research Database (Denmark)

    Oryema, Christine; Oryem-Origa, Hannington; Roos, Nanna

    2016-01-01

    Vitellaria paradoxa Gaertn locally known as ‘Yaa’ in Acholi is a valuable edible indigenous wild fruit in Gulu District, northern Uganda. It is a multipurpose fruit tree and highly favoured by the inhabitants of this district. Its fruit pulps are eaten when fresh and/or made into cakes and dried....... This study determined the nutrient compositions of the fresh pulps and dried pulp cakes of V. paradoxa. Laboratory analyses were undertaken to determine the micro and macro nutrients in the samples on fresh (FM) and dry matter (DM) basis following standard procedures and protocols. The potassium, sodium...

  8. Effect of Bagasse Chemical Pulping and Coupling Agent on the Physical - Mechanical Properties of Composites Based on Bagasse pulp/Low density polyethylene

    Directory of Open Access Journals (Sweden)

    maryam allahdadi

    2016-12-01

    Full Text Available In this research, effect of reinforcing bagasse pulp and raw bagasse fibers and applying coupling agent MAPE (Maleic Anhydride Polyethylene on physical-mechanical properties of low density polyethylene (LDPE composites were studided. Fresh bagasse were collected from an experimental field in Khuzestan and after investigating anatomy and chemical properties of Different pulp fibers including monoethanolamine (MEA bagasse pulp, alkaline sulfite-anthraquinone (AS bagasse pulp, bleached soda (BS bagasse pulp, unbleached soda (UNS bagasse pulp and raw bagasse fibers (B were prepared. Then, composites with 30wt.% fiber content were manufactured by twin-screw extrusion followed by compression molding processing. The mechanical and physical properties of these composites were analyzed and compared. Results revealed that the chemical pulping dissolved a fraction of lignin and hemicelluloses so that the linkage potential and aspect ratio of bagasse fibers was improved and consequently, as compared with the raw bagasse fibers, bagasse pulp fibers have better reinforcing capability. The best overall properties were achieved with MEA and AS/AQ fibers. Addition of 5% (wt/wt of coupling agent MAPE resulted in a significant enhancement in the tensile strength, tensile modulus and impact strength in line with the improvement of the fiber-matrix interfacial adhesion making more effective the transfer of stress from the matrix to the rigid reinforcement.

  9. PULP OBTAINING METHOD FOR PACKAGE PRODUCTION

    Directory of Open Access Journals (Sweden)

    V. V. Kuzmich

    2015-01-01

    Full Text Available The paper presents a new method for obtaining pulp which is used for production of cardboard, paper and package while using carbon dioxide and hydrazine hydrate and neutral-sulfite  shive cooking. Output increase of  the desired product can be explained by reduction in destruction of plant raw material carbohydrates during its cooking process. Quality improvement of the desired product (improvement in bleaching and output is attributed to the fact that usage of carbon dioxide and hydrazine contributes to provision of polysaccharide chain resistance to destruction due to the presence of  end links having structure of metasaccharinic and aldonic acids.The author has developed a new method for pulp obtaining on the basis of the executed investigations  and literature data.  СО2 and hydrazine hydrate have been used for obtaining pulp. Method invention concerns pulp obtaining and it can be used for paper and cardboard package manufacturing in pulp and paper industry.The method is to be carried in the following way: pulp-containing plant raw material is loaded into an autoclave and then aqua solution of sodium monosulfite containing hydrazine hydrate that constitutes 4–5 % of absolute dry pulp-containing raw material mass with liquid module 1:6–1:8 is supplied into the autoclave. The autoclave is closed for operation under pressure and the solution is carbonated under pressure which constitutes 5–8 % of absolute dry plant raw material (shover. Temperature is subsequently raised up to 180 °С in the space of 2 hours and cooking is carried out in the course of 4 hours. Usage of  the proposed method for shover cooking makes it possible to reduce monosulfite cooking process and improve qualitative characteristics and output of the desired product.  In addition to above mentioned fact there is a possibility to improve bleaching and final product output. 

  10. Functionalized scaffolds to control dental pulp stem cell fate

    Science.gov (United States)

    Piva, Evandro; Silva, Adriana F.; Nör, Jacques E.

    2014-01-01

    Emerging understanding about interactions between stem cells, scaffolds and morphogenic factors has accelerated translational research in the field of dental pulp tissue engineering. Dental pulp stem cells constitute a sub-population of cells endowed with self-renewal and multipotency. Dental pulp stem cells seeded in biodegradable scaffolds and exposed to dentin-derived morphogenic signals give rise to a pulp-like tissue capable of generating new dentin. Notably, dentin-derived proteins are sufficient to induce dental pulp stem cell differentiation into odontoblasts. Ongoing work is focused on developing ways of mobilizing dentin-derived proteins and disinfecting the root canal of necrotic teeth without compromising the morphogenic potential of these signaling molecules. On the other hand, dentin by itself does not appear to be capable of inducing endothelial differentiation of dental pulp stem cells, despite the well known presence of angiogenic factors in dentin. This is particularly relevant in the context of dental pulp tissue engineering in full root canals, where access to blood supply is limited to the apical foramina. To address this challenge, scientists are looking at ways to use the scaffold as a controlled release device for angiogenic factors. The aim of this manuscript is to present and discuss current strategies to functionalize injectable scaffolds and customize them for dental pulp tissue engineering. The long-term goal of this work is to develop stem cell-based therapies that enable the engineering of functional dental pulps capable of generating new tubular dentin in humans. PMID:24698691

  11. Potential for biohydrogen and methane production from olive pulp

    DEFF Research Database (Denmark)

    Gavala, Hariklia N.; Skiadas, Ioannis V.; Ahring, Birgitte Kiær

    2005-01-01

    The present study investigates the potential for thermophilic biohydrogen and methane production from olive pulp, which is the semi-solid residue coming from the two-phase processing of olives. It focussed on: a) production of methane from the raw olive pulp, b) anaerobic bio-production of hydrogen...... from the olive pulp, and c) subsequent anaerobic treatment of the hydrogen-effluent with the simultaneous production of methane. Both continuous and batch experiments were performed. The hydrogen potential of the olive pulp amounted to 1.6 mmole H-2 per g TS. The methane potential of the raw olive pulp...... and hydrogen-effluent was as high as 19 mmole CH4 per g TS. This suggests that olive pulp is an ideal substrate for methane production and it shows that biohydrogen production can be very efficiently coupled with a subsequent step for methane production....

  12. Two-phase synthesis of hydrophobic ionic liquid-capped gold nanoparticles and their application for sensing cholesterol

    International Nuclear Information System (INIS)

    Dong, Mingjun; Nan, Zhihan; Liu, Panpan; Zhang, Yanjun; Xue, Zhonghua; Lu, Xiaoquan; Liu, Xiuhui

    2014-01-01

    Highlights: • A novel cholesterol biosensor was constructed based on ChOx-IL-capped-AuNPs/GCE. • IL-capped-AuNPs was synthesized using two-phase synthesis and employed as a conducting matrix to immobilize ChOx. • Direct electrochemistry of ChOx on the electrode was obtained. • The ChOx-IL-capped-AuNPs/GCE exhibit remarkable performance for cholesterol detection. - Abstract: A novel scheme for fabrication of hydrophobic ionic liquid-capped gold nanoparticles (IL-capped AuNPs) modified electrode is presented and its application potential for cholesterol biosensor is investigated. Highly stable gold nanoparticles were characterized by UV–vis absorption spectroscopy and transmission electron microscopy (TEM). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) indicated that IL-capped AuNPs nanocomposites showed excellent electrical conductivity. Furthermore, cholesterol oxidase (ChOx) was directly immobilized on the IL-capped AuNPs nanocomposite, and then the direct electrochemistry of ChOx on the modified glass carbon electrode (GCE) was obtained. As a new platform in cholesterol analysis, ChOx-IL-capped AuNPs/GCE exhibited a linear response to cholesterol in the range of 0.1–50 μM with a detection limit of 0.033 μM. Therefore, hydrophobic ionic liquid-capped gold nanoparticles would serve as a good candidate material to construct the related enzyme biosensors

  13. Sisal organosolv pulp as reinforcement for cement based composites

    OpenAIRE

    Joaquim, Ana Paula; Tonoli, Gustavo Henrique Denzin; Santos, Sérgio Francisco Dos; Savastano Junior, Holmer

    2009-01-01

    The present work describes non-conventional sisal (Agave sisalana) chemical (organosolv) pulp from residues of cordage as reinforcement to cement based materials. Sisal organosolv pulp was produced in a 1:1 ethanol/water mixture and post chemically and physically characterized in order to compare its properties with sisal kraft pulp. Cement based composites reinforced with organosolv or kraft pulps and combined with polypropylene (PP) fibres were produced by the slurry de-watering and pressin...

  14. Radiation modification of cellulose pulps. Preparation of cellulose derivatives

    International Nuclear Information System (INIS)

    Iller, E.; Zimek, Z.; Stupinska, H.; Mikolajczyk, W; Starostka, P.

    2005-01-01

    One of the most common methods of cellulose pulp modification (activation) applied in the production process of cellulose derivatives is the treatment of the pulp with NaOH solutions leading to the formation of alkalicellulose. The product then undergoes a prolonged process of maturation by its storage under specific conditions. The goal of the process is lowering of the molecular weight of cellulose down to the level resulting from various technological requirements. The process is time-consuming and costly; besides, it requires usage of large-capacity technological vessels and produces considerable amounts of liquid waste. Therefore, many attempts have been made to limit or altogether eliminate the highly disadvantageous stage of cellulose treatment with lye. One of the alternatives proposed so far is the radiation treatment of the cellulose pulp. In the pulp exposed to an electron beam, the bonds between molecules of D-antihydroglucopiranoses loosen and the local crystalline lattice becomes destroyed. This facilitates the access of chemical reagents to the inner structure of the cellulose and, in consequence, eliminates the need for the prolonged maturation of alkalicellulose, thus reducing the consumption of chemicals by the whole process. Research aimed at the application of radiation treatment of cellulose pulp for the production of cellulose derivatives has been conducted by a number of scientific institutions including the Institute of Nuclear Chemistry and Technology, Institute of Biopolymers and Chemical Fibres, and Pulp and Paper Research Institute. For the investigations and assessment of the molecular, hypermolecular, morphologic properties and the chemical reactivity, cellulose pulps used for chemical processing, namely Alicell, Borregaard and Ketchikan, as well as paper pulps made from pine and birch wood were selected. The selected cellulose pulps were exposed to an electron beam with an energy of 10 MeV generated in a linear electron accelerator

  15. STUDIES ON HIBISCUS CANNABINUS, HIBISCUS SABDARIFFA, AND CANNABINUS SATIVA PULP TO BE A SUBSTITUTE FOR SOFTWOOD PULP- PART 1: AS-AQ DELIGNIFICATION PROCESS

    OpenAIRE

    Dharm Dutt; J. S. Upadhyaya; C. H. Tyagi

    2010-01-01

    Hibiscus cannabinus, Hibiscus sabdariffa, and Cannabinus sativa, which are renewable non-woody fiber resources having characteristics similar to that of softwood (bast fibers), when used together with hardwood (core fibers), gave higher pulp yield with good mechanical strength properties when using an alkaline sulphite-anthraquinone (AS-AQ) pulping process rather than a conventional kraft pulping process and bleached more readily than kraft and soda pulps with a CEHH bleaching sequence. A com...

  16. Static Magnetic Field Attenuates Lipopolysaccharide-Induced Inflammation in Pulp Cells by Affecting Cell Membrane Stability

    Directory of Open Access Journals (Sweden)

    Sung-Chih Hsieh

    2015-01-01

    Full Text Available One of the causes of dental pulpitis is lipopolysaccharide- (LPS- induced inflammatory response. Following pulp tissue inflammation, odontoblasts, dental pulp cells (DPCs, and dental pulp stem cells (DPSCs will activate and repair damaged tissue to maintain homeostasis. However, when LPS infection is too serious, dental repair is impossible and disease may progress to irreversible pulpitis. Therefore, the aim of this study was to examine whether static magnetic field (SMF can attenuate inflammatory response of dental pulp cells challenged with LPS. In methodology, dental pulp cells were isolated from extracted teeth. The population of DPSCs in the cultured DPCs was identified by phenotypes and multilineage differentiation. The effects of 0.4 T SMF on DPCs were observed through MTT assay and fluorescent anisotropy assay. Our results showed that the SMF exposure had no effect on surface markers or multilineage differentiation capability. However, SMF exposure increases cell viability by 15%. In addition, SMF increased cell membrane rigidity which is directly related to higher fluorescent anisotropy. In the LPS-challenged condition, DPCs treated with SMF demonstrated a higher tolerance to LPS-induced inflammatory response when compared to untreated controls. According to these results, we suggest that 0.4 T SMF attenuates LPS-induced inflammatory response to DPCs by changing cell membrane stability.

  17. Emerging Energy-Efficiency and Greenhouse Gas Mitigation Technologies for the Pulp and Paper Industry

    Energy Technology Data Exchange (ETDEWEB)

    Kong, Lingbo [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); South China Univ. of Technology (SCUT), Guangzhou (China); Hasanbeigi, Ali [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Price, Lynn [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2012-11-01

    The pulp and paper industry ranks fourth in terms of energy consumption among industries worldwide. Globally, the pulp and paper industry accounted for approximately 5 percent of total world industrial final energy consumption in 2007, and contributed 2 percent of direct carbon dioxide (CO2) emissions from industry. Worldwide pulp and paper demand and production are projected to increase significantly by 2050, leading to an increase in this industry’s absolute energy use and greenhouse gas (GHG) emissions. Development of new energy-efficiency and GHG mitigation technologies and their deployment in the market will be crucial for the pulp and paper industry’s mid- and long-term climate change mitigation strategies. This report describes the industry’s processes and compiles available information on the energy savings, environmental and other benefits, costs, commercialization status, and references for 36 emerging technologies to reduce the industry’s energy use and GHG emissions. Although studies from around the world identify a variety of sector-specific and cross-cutting energy-efficiency technologies that have already been commercialized for the pulp and paper industry, information is scarce and/or scattered regarding emerging or advanced energy-efficiency and low-carbon technologies that are not yet commercialized. The purpose of this report is to provide engineers, researchers, investors, paper companies, policy makers, and other interested parties with easy access to a well-structured resource of information on these technologies.

  18. Organosolv pulping and test paper characterization of fiber hemp

    NARCIS (Netherlands)

    Zomers, F.H.A.; Gosselink, R.J.A.; Dam, van J.E.G.; Tjeerdsma, B.F.

    1995-01-01

    The autocatalyzed ethanol pulping of hemp to produce chemical-grade pulp was examined. Batch delignification conditions were developed for hemp core, hemp bast, and hemp whole stem. Although successful pulping of both hemp core and bast fibre is possible, further research is necessary.

  19. Substance P and CGRP expression in dental pulps with irreversible pulpitis.

    Science.gov (United States)

    Sattari, Mandana; Mozayeni, Mohammad Ali; Matloob, Arash; Mozayeni, Maryam; Javaheri, Homan H

    2010-08-01

    The purpose of this study was to compare substance P (SP) and calcitonin gene-related peptide (CGRP) expression in pulp tissue with clinically diagnosed symptomatic and asymptomatic irreversible pulpitis. Healthy pulps acted as controls. Five normal pulps and 40 with irreversible pulpitis (20 symptomatic and 20 asymptomatic) were obtained from 45 different patients. SP and CGRP expression was determined by competition binding assays using enzyme immunoassay. anova and Mann-Whitney tests were used to ascertain if there were statistically significant differences between the groups. The results showed that neuropeptides were found in all pulp samples. The highest and the lowest expressions for SP and CGRP were found in symptomatic irreversible pulpitis and healthy pulps groups, respectively. The differences between healthy pulps and the groups of pulps having irreversible pulpitis were significant (P pulpitis groups (P pulpitis groups were not significant. This study demonstrated that the expression of CGRP and SP is significantly higher in pulps with irreversible pulpitis compared with healthy pulps.

  20. The thickness of odontoblast-like cell layer after induced by propolis extract and calcium hydroxide

    Directory of Open Access Journals (Sweden)

    Irfan Dwiandhono

    2016-12-01

    Full Text Available Background: Propolis is a substance made from resin collected by bees (Apis mellifera from variety of plants, mixed with its saliva and various enzymes to build a nest. Propolis has potential antimicrobial and antiinflammatory agents with some advantages over calcium hydroxide (Ca(OH2. Ca(OH2 has been considered as the “gold standard” of direct pulp-capping materials, but there are still some weakness of its application. First, it can induce pulp inflammation which last up to 3 months. Second, the tissue response to Ca(OH2 is not always predictable. Third, the tunnel defect can probably formed in dentinal bridge with possible bacterial invasion in that gap. Purpose: This study was aimed to determine and compare the thickness of odontoblast-like cells layer after induced by propolis extract and Ca(OH2 in rat’s pulp tissue. Method: Class 1 preparation was done in maxillary first molar tooth of wistar mice until the pulp opened. The Ca(OH2 and propolis extract was applied to induce the formation of odontoblast-like cells, the cavity was filled with RMGIC. The teeth were extracted (after 14 and 28 days of induction. The samples were then processed for histological evaluation. Result: There were significant differences between the thickness of odontoblast-like cells after induced by propolis extract and Ca(OH2. Conclusion: The propolis extract as the direct pulp capping agent produces thicker odontoblast-like cell layer compared to Ca(OH2.

  1. Investigation of pulping and paper making potential of weeds

    Directory of Open Access Journals (Sweden)

    omid Ghaffarzadeh Mollabashi

    2017-08-01

    Full Text Available Increasing use of wood products accompanying with resource constraint has revealed the importance of nonwood based material. In this study, pulping and papermaking potential of three varieties of weeds including Xanthium spinosum, Carthamus tinctorius and Cyperus papyrus have been considered. At first, chemical components of the samples i.e. cellulose, lignin and extractives have been measured following TAPPI standard test methods. Afterwards, pulping process based as soda and Kraft has been carried out and the pulp properties i.e. screen yield and reject, kappa number, caliper, bust index, tear index, brightness have been considered. According to the results, the amount cellulose, lignin and extractives have been measured for the Xanthium spinosum %38.15, %13.5 and 4.72, respectively. Theses parameters have been estimated about %38.25, %10.3 and % 2.95 for Carthamus tinctorius and %38.8, %19.2 and 4.4 in case of papyrus. The yield of soda and Kraft pulp of the papyrus was more than Xanthium spinosum and Carthamus tinctorius. Among all treatments, the highest screen yield related to soda pulping of Cyperus papyrus by %39.8 which has been obtained by 175 centigrade as a maximum temperature, L/W: 6/1, active alkaline: %30 and 90 minutes as the time at temperature. The lowest and highest amounts of the tear index were related to soda pulp sample of the Carthamus tinctorius and Kraft pulp sample of Xanthium spinosum by 2.49 and 8.1, respectively. In addition, the lowest and highest amounts of the bursting index were related to soda pulp sample of the Cyperus papyrus and Kraft pulp sample of Xanthium spinosum by 0.61and 2.48, respectively. Meanwhile, soda pulp sample of the Cyperus papyrus showed the highest amount of brightness with %45 ISO.

  2. Effect of Urea Addition on Soda Pulping of Oak Wood

    OpenAIRE

    Cho, Nam-Seok; Matsumoto, Yuji; Cho, Hee-Yeon; Shin, Soo-Jeong; Oga, Shoji

    2008-01-01

    Many studies have been conducted to find a sulfur-free additive for alkaline pulping liquors that would have an effect similar to that of sulfide in kraft pulping. Some reagents that partially fulfill this role have been found, but they are too expensive to be used in the quantities required to make them effective. As an alternative method to solve air pollution problem and difficulty of pulp bleaching of kraft pulping process, NaOH-Urea pulping was applied. The properties of NaOH-Urea pul...

  3. A modified efficient method for dental pulp stem cell isolation.

    Science.gov (United States)

    Raoof, Maryam; Yaghoobi, Mohammad Mehdi; Derakhshani, Ali; Kamal-Abadi, Ali Mohammadi; Ebrahimi, Behnam; Abbasnejad, Mehdi; Shokouhinejad, Noushin

    2014-03-01

    Dental pulp stem cells can be used in regenerative endodontic therapy. The aim of this study was to introduce an efficient method for dental pulp stem cells isolation. In this in-vitro study, 60 extracted human third molars were split and pulp tissue was extracted. Dental pulp stem cells were isolated by the following three different methods: (1) digestion of pulp by collagenase/dispase enzyme and culture of the released cells; (2) outgrowth of the cells by culture of undigested pulp pieces; (3) digestion of pulp tissue pieces and fixing them. The cells were cultured in minimum essential medium alpha modification (αMEM) medium supplemented with 20% fetal bovine serum(FBS) in humid 37°C incubator with 5% CO 2. The markers of stem cells were studied by reverse transcriptase polymerase chain reaction (PCR). The student t-test was used for comparing the means of independent groups. P third method, we obtained stem cells successfully with about 60% efficiency after 2 days. The results of RT-PCR suggested the expression of Nanog, Oct-4, and Nucleostemin markers in the isolated cells from dental pulps. This study proposes a new method with high efficacy to obtain dental pulp stem cells in a short time.

  4. Cradle Cap: Treatment

    Science.gov (United States)

    Cradle cap Treatment Cradle cap usually doesn't require medical treatment. It clears up on its own within a few months. In the meantime, wash ... tips can help you control and manage cradle cap. Gently rub your baby's scalp with your fingers ...

  5. Rapid determination of fluorine in the pulp process

    International Nuclear Information System (INIS)

    Ivanenko, V.V.; Kustov, V.N.; Levitskaya, O.N.

    1994-01-01

    The control of enrichment of mineral raw materials in order to increase the yield of enriched minerals is an important problem of modern industry. A possible solution is an automatic control system (ACS), which makes possible the monitoring and optimization of the enrichment. In terms of future applications, the nuclear-physical analysis, which is based on the use of a radionuclide source of neutrons, is a promising method. The advantages of this method (high rate, the possibility of performing the analysis directly in the flow, high accuracy, and high sensitivity) make themselves evident in the determination of short-lived isotopes, such as F, Ag, Si, Al, V, etc. Both an instrumental complex and a method for the rapid neutron activation determination (RNAD) of fluorine in the pulp flow of the enrichment process of raw materials were developed at the Institute of Chemistry, Far East Division, Russian Academy of Sciences. The analytical complex for RNAD comprises an activator; a densitometer; a pump; a pulp-feed system; a gamma-ray detector; a data acquisition apparatus; and a computer for processing the input/output data and controlling the analysis. The authors found that the RNAD of fluorine using radionuclide Pu-Be sources allows the determination in a 6-L pulp under recirculation conditions with an error of less than 1% in 3-5 minutes. The method allows the routine monitoring of the enrichment process of mineral raw materials

  6. An investigation of dentinal fluid flow in dental pulp during food mastication: simulation of fluid-structure interaction.

    Science.gov (United States)

    Su, Kuo-Chih; Chuang, Shu-Fen; Ng, Eddie Yin-Kwee; Chang, Chih-Han

    2014-06-01

    This study uses fluid-structure interaction (FSI) simulation to investigate the relationship between the dentinal fluid flow in the dental pulp of a tooth and the elastic modulus of masticated food particles and to investigate the effects of chewing rate on fluid flow in the dental pulp. Three-dimensional simulation models of a premolar tooth (enamel, dentine, pulp, periodontal ligament, cortical bone, and cancellous bone) and food particle were created. Food particles with elastic modulus of 2,000 and 10,000 MPa were used, respectively. The external displacement loading (5 μm) was gradually directed to the food particle surface for 1 and 0.1 s, respectively, to simulate the chewing of food particles. The displacement and stress on tooth structure and fluid flow in the dental pulp were selected as evaluation indices. The results show that masticating food with a high elastic modulus results in high stress and deformation in the tooth structure, causing faster dentinal fluid flow in the pulp in comparison with that obtained with soft food. In addition, fast chewing of hard food particles can induce faster fluid flow in the pulp, which may result in dental pain. FSI analysis is shown to be a useful tool for investigating dental biomechanics during food mastication. FSI simulation can be used to predict intrapulpal fluid flow in dental pulp; this information may provide the clinician with important concept in dental biomechanics during food mastication.

  7. Survey of Enabling Technologies for CAPS

    Science.gov (United States)

    Antol, Jeffrey; Mazanek, Daniel D.; Koons, Robert H.

    2005-01-01

    The enabling technologies required for the development of a viable Comet/Asteroid Protection System (CAPS) can be divided into two principal areas: detection and deflection/orbit modification. With the proper funding levels, many of the technologies needed to support a CAPS architecture could be achievable within the next 15 to 20 years. In fact, many advanced detection technologies are currently in development for future in-space telescope systems such as the James Webb Space Telescope (JWST), formerly known as the Next Generation Space Telescope. It is anticipated that many of the JWST technologies would be available for application for CAPS detection concepts. Deflection/orbit modification technologies are also currently being studied as part of advanced power and propulsion research. However, many of these technologies, such as extremely high-output power systems, advanced propulsion, heat rejection, and directed energy systems, would likely be farther term in availability than many of the detection technologies. Discussed subsequently is a preliminary examination of the main technologies that have been identified as being essential to providing the element functionality defined during the CAPS conceptual study. The detailed requirements for many of the technology areas are still unknown, and many additional technologies will be identified as future in-depth studies are conducted in this area.

  8. Greening CAP payments: a missed opportunity?

    Energy Technology Data Exchange (ETDEWEB)

    Matthews, Alan

    2013-01-15

    At an important point in the current reform of the Common Agricultural Policy (CAP), a new IIEA policy brief by Professor Alan Matthews, one of the EU’s foremost experts on the topic, considers proposals to green direct farm payments. Professor Matthews argues that proposed greening of direct payments – the key innovation in the current round of CAP Reform – look likely to fail. While greening may survive as a concept, the likely outcome of the negotiations between Agriculture Ministers and the European Parliament will deliver little practical environmental benefit. The paper examines the rationale underpinning greening, arguing that it exists to justify the continuation of a large agricultural budget, explores reasons for the apparent failure of the proposals, and reflects on the implications for future efforts to better integrate environmental objectives into EU agriculture policy. This is the first in a series of Environment Nexus policy briefs by leading experts in the fields of agriculture, energy, climate change and water.

  9. Pulp quality from small-diameter trees.

    Science.gov (United States)

    G.C. Myers; S. Kumar; R.R. Gustafson; R.J. Barbour; S.M. Abubakr

    1997-01-01

    Kraft and thermomechanical (TMP) pulps were prepared and evaluated from lodgepole pine and mixed Douglas-fir/western larch sawmill residue chips; lodgepole pine, Douglas-fir, and western larch submerchantable logs; and lodgepole pine, Douglas-fir, and western larch small trees and tops. Kraft pulp from small trees and tops was identical to that from submerchantable...

  10. Environmental performance of straw-based pulp making: A life cycle perspective.

    Science.gov (United States)

    Sun, Mingxing; Wang, Yutao; Shi, Lei

    2018-03-01

    Agricultural straw-based pulp making plays a vital role in pulp and paper industry, especially in forest deficient countries such as China. However, the environmental performance of straw-based pulp has scarcely been studied. A life cycle assessment on wheat straw-based pulp making in China was conducted to fill of the gaps in comprehensive environmental assessments of agricultural straw-based pulp making. On average, the global warming potential (GWP), GWP excluding biogenic carbon, acidification potential and eutrophication potential of wheat straw based pulp making are 2299kg CO 2 -eq, 4550kg CO 2 -eq, 16.43kg SO 2 -eq and 2.56kg Phosphate-eq respectively. The dominant factors contributing to environmental impacts are coal consumption, electricity consumption, and chemical (NaOH, ClO 2 ) input. Chemical input decrease and energy recovery increase reduce the total environmental impacts dramatically. Compared with wood-based and recycled pulp making, wheat straw-based pulp making has higher environmental impacts, which are mainly due to higher energy and chemical requirements. However, the environmental impacts of wheat straw-based pulp making are lower than hemp and flax based pulp making from previous studies. It is also noteworthy that biogenic carbon emission is significant in bio industries. If carbon sequestration is taken into account in pulp making industry, wheat straw-based pulp making is a net emitter rather than a net absorber of carbon dioxide. Since wheat straw-based pulp making provides an alternative for agricultural residue management, its evaluation framework should be expanded to further reveal its environmental benefits. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Multivariate Analysis of Hemicelluloses in Bleached Kraft Pulp Using Infrared Spectroscopy.

    Science.gov (United States)

    Chen, Zhiwen; Hu, Thomas Q; Jang, Ho Fan; Grant, Edward

    2016-12-01

    The hemicellulose composition of a pulp significantly affects its chemical and physical properties and thus represents an important process control variable. However, complicated steps of sample preparation make standard methods for the carbohydrate analysis of pulp samples, such as high performance liquid chromatography (HPLC), expensive and time-consuming. In contrast, pulp analysis by attenuated total internal reflection Fourier transform infrared spectroscopy (ATR FT-IR) requires little sample preparation. Here we show that ATR FT-IR with discrete wavelet transform (DWT) and standard normal variate (SNV) spectral preprocessing offers a convenient means for the qualitative and quantitative analysis of hemicelluloses in bleached kraft pulp and alkaline treated kraft pulp. The pulp samples investigated include bleached softwood kraft pulps, bleached hardwood kraft pulps, and their mixtures, as obtained from Canadian industry mills or blended in a lab, and bleached kraft pulp samples treated with 0-6% NaOH solutions. In the principal component analysis (PCA) of these spectra, we find the potential both to differentiate all pulps on the basis of hemicellulose compositions and to distinguish bleached hardwood pulps by species. Partial least squares (PLS) multivariate analysis gives a 0.442 wt% root mean square errors of prediction (RMSEP) for the prediction of xylan content and 0.233 wt% RMSEP for the prediction of mannan content. These data all support the idea that ATR FT-IR has a great potential to rapidly and accurately predict the content of xylan and mannan for bleached kraft pulps (softwood, hardwood, and their mixtures) in industry. However, the prediction of xylan and mannan concentrations presented a difficulty for pulp samples with modified cellulose crystalline structure. © The Author(s) 2016.

  12. Positive and negative aspects of soda/anthraquinone pulping of hardwoods.

    Science.gov (United States)

    Francis, R C; Bolton, T S; Abdoulmoumine, N; Lavrykova, N; Bose, S K

    2008-11-01

    The positive aspects of the non-sulfur soda/anthraquinone (SAQ) process are mostly tied to improved energy efficiency while lower pulp brightness after bleaching is its most significant drawback. A credible method that quantifies bleachability as well as an approach that solves the problem for SAQ pulps from hardwoods will be described. A straight line correlation (R2=0.904) was obtained between O2 kappa number and final light absorption coefficient (LAC) value after standardized OD0EpD1 bleaching of nine hardwood kraft pulps from three laboratories and one pulp mill. The bleachability of pulps from four different soda processes catalyzed by anthraquinone (AQ) and 2-methylanthraquinone (MAQ) was compared to that of conventional kraft pulps by comparing O2 kappa number decrease and final LAC values. It was observed that a mild hot water pre-hydrolysis improved the bleachability of SAQ pulps to a level equal to that of kraft.

  13. Integration of the Mini-Sulfide Sulfite Anthraquinone (MSS-AQ) Pulping Process and Black Liquor Gasification in a Pulp Mill

    Energy Technology Data Exchange (ETDEWEB)

    Hasan Jameel, North Carolina State University; Adrianna Kirkman, North Carolina State University; Ravi Chandran,Thermochem Recovery International Brian Turk Research Triangle Institute; Brian Green, Research Triangle Institute

    2010-01-27

    As many of the recovery boilers and other pieces of large capital equipment of U.S. pulp mills are nearing the end of their useful life, the pulp and paper industry will soon need to make long-term investments in new technologies. The ability to install integrated, complete systems that are highly efficient will impact the industry’s energy use for decades to come. Developing a process for these new systems is key to the adoption of state-of-the-art technologies in the Forest Products industry. This project defined an integrated process model that combines mini-sulfide sulfite anthraquinone (MSS-AQ) pulping and black liquor gasification with a proprietary desulfurization process developed by the Research Triangle Institute. Black liquor gasification is an emerging technology that enables the use of MSS-AQ pulping, which results in higher yield, lower bleaching cost, lower sulfur emissions, and the elimination of causticization requirements. The recently developed gas cleanup/absorber technology can clean the product gas to a state suitable for use in a gas turbine and also regenerate the pulping chemicals needed to for the MSS-AQ pulping process. The combination of three advanced technologies into an integrated design will enable the pulping industry to achieve a new level of efficiency, environmental performance, and cost savings. Because the three technologies are complimentary, their adoption as a streamlined package will ensure their ability to deliver maximum energy and cost savings benefits. The process models developed by this project will enable the successful integration of new technologies into the next generation of chemical pulping mills. When compared to the Kraft reference pulp, the MSS-AQ procedures produced pulps with a 10-15 % yield benefit and the ISO brightness was 1.5-2 times greater. The pulp refined little easier and had a slightly lower apparent sheet density (In both the cases). At similar levels of tear index the MSS-AQ pulps also

  14. Paleoproteomics of the Dental Pulp: The plague paradigm.

    Science.gov (United States)

    Barbieri, Rémi; Mekni, Rania; Levasseur, Anthony; Chabrière, Eric; Signoli, Michel; Tzortzis, Stéfan; Aboudharam, Gérard; Drancourt, Michel

    2017-01-01

    Chemical decomposition and fragmentation may limit the detection of ancient host and microbial DNA while some proteins can be detected for extended periods of time. We applied paleoproteomics on 300-year-old dental pulp specimens recovered from 16 individuals in two archeological funeral sites in France, comprising one documented plague site and one documented plague-negative site. The dental pulp paleoproteome of the 16 teeth comprised 439 peptides representative of 30 proteins of human origin and 211 peptides representative of 27 proteins of non-human origin. Human proteins consisted of conjunctive tissue and blood proteins including IgA immunoglobulins. Four peptides were indicative of three presumable Yersinia pestis proteins detected in 3/8 dental pulp specimens from the plague-positive site but not in the eight dental pulp specimens collected in the plague-negative site. Paleoproteomics applied to the dental pulp is a new and innovative approach to screen ancient individuals for the detection of blood-borne pathogens and host inflammatory response.

  15. The influence of the extraction parameters on the quality of dried sugar beet pulp

    Directory of Open Access Journals (Sweden)

    Bogdanović Branislav V.

    2013-01-01

    Full Text Available Growing demand for better quality and cheaper products put on the need to industry to balance between the two kinds of needs, which are the reduction of production costs and to ensure a high quality product. This is the case with dried sugar beet pulp as a byproduct of sugar refining process. One of the possibilities to reduce energy consumption is savings in the process of thermal dehydration in the process of the conventional drying of pulp. Pulp drying is one third of total energy consumption in the sugar factory. Acidification of water extraction result in pulp that could be pressed better and in other hand in the juice with less non-sucrose compounds. Keeping the extraction process under certain conditions, directly affects on the quality of the extracted pulp and on effects of their further processing. This paper examines the impact of the extraction parameters of sugar from sugar beet and agents of acidification for water extraction (sulphurous acid, hydrogen peroxide on the quality of dried sugar beet pulp. Extraction of sugar from sugar beet cossettes by hydrogen peroxide at pH 5.5 to 8.5 does not cause changes in mechanical properties of sugar beet cossettes and in water retention coefficient, but increases the brightness of dried sugar beet pulp for about 5-7 units, which solubility in water does not depend on the applied concentration of extracting agents, but increases in dependence on the duration of the extraction. Sugar beet pulp extracted at pH 11.0 has reduced strength, they are highly hydrated, and under the force they lose their shape, binding a larger amount of water thus reducing the effect of their pressing. The changes in microstructure were probably accompanied by segmenting the linear parts of macromolecules and are reflected in the increase of water retention capacity and increase the solubility of dried sugar beet pulp for two to three times. Under the same conditions of extraction, sulphurous acid produces similar

  16. Standard and alternative landfill capping design in Germany

    International Nuclear Information System (INIS)

    Simon, Franz-Georg; Mueller, Werner W.

    2004-01-01

    Engineered capping systems are in most cases an indispensable and often the only efficient component required by the long-term safety concept for landfills, mine tailings tips and contaminated land. In Germany the composite liner is the main component of standard landfill cappings for municipal and hazardous waste landfills and the compacted clay liner (CCL) for landfills for inert or low-contamination waste. The composite liner is a technically highly effective but very expensive system. Research and experience has given rise to concern about the proper long-term performance of a conventional single CCL as a landfill capping. Therefore, alternative capping systems are discussed and applied for landfills and for the containment of contaminated sites. This paper gives an overview on various alternative engineered cappings and suitable systems for capping reflecting the state of the art and the expert view in Germany. According to the European Council Directive on the landfill of waste an impermeable mineral layer is recommended for the surface sealing of non-hazardous landfills and a composition of artificial sealing liner and impermeable mineral layer for hazardous landfills. In both cases a drainage layer thickness of at least 0.5 m is suggested. These recommendations should be interpreted flexibly and to some extent modified in the light of the experience and results presented in this paper

  17. Pulp-dentin Regeneration: Current State and Future Prospects.

    Science.gov (United States)

    Cao, Y; Song, M; Kim, E; Shon, W; Chugal, N; Bogen, G; Lin, L; Kim, R H; Park, N-H; Kang, M K

    2015-11-01

    The goal of regenerative endodontics is to reinstate normal pulp function in necrotic and infected teeth that would result in reestablishment of protective functions, including innate pulp immunity, pulp repair through mineralization, and pulp sensibility. In the unique microenvironment of the dental pulp, the triad of tissue engineering would require infection control, biomaterials, and stem cells. Although revascularization is successful in resolving apical periodontitis, multiple studies suggest that it alone does not support pulp-dentin regeneration. More recently, cell-based approaches in endodontic regeneration based on pulpal mesenchymal stem cells (MSCs) have demonstrated promising results in terms of pulp-dentin regeneration in vivo through autologous transplantation. Although pulpal regeneration requires the cell-based approach, several challenges in clinical translation must be overcome-including aging-associated phenotypic changes in pulpal MSCs, availability of tissue sources, and safety and regulation involved with expansion of MSCs in laboratories. Allotransplantation of MSCs may alleviate some of these obstacles, although the long-term stability of MSCs and efficacy in pulp-dentin regeneration demand further investigation. For an alternative source of MSCs, our laboratory developed induced MSCs (iMSCs) from primary human keratinocytes through epithelial-mesenchymal transition by modulating the epithelial plasticity genes. Initially, we showed that overexpression of ΔNp63α, a major isoform of the p63 gene, led to epithelial-mesenchymal transition and acquisition of stem characteristics. More recently, iMSCs were generated by transient knockdown of all p63 isoforms through siRNA, further simplifying the protocol and resolving the potential safety issues of viral vectors. These cells may be useful for patients who lack tissue sources for endogenous MSCs. Further research will elucidate the level of potency of these iMSCs and assess their

  18. Dental Pulp: Correspondences and Contradictions between Clinical and Histological Diagnosis

    Science.gov (United States)

    Giuroiu, Cristian Levente; Căruntu, Irina-Draga; Lozneanu, Ludmila; Melian, Anca; Vataman, Maria; Andrian, Sorin

    2015-01-01

    Dental pulp represents a specialized connective tissue enclosed by dentin and enamel, the most highly mineralized tissues of the body. Consequently, the direct examination as well as pathological evaluation of dental pulp is difficult. Within this anatomical context, our study aimed to evaluate the correlation between dental pulp lesions and clinical diagnosis. Pulpectomies were performed for 54 patients with acute and chronic irreversible pulpitides and for 5 patients (control group) with orthodontic extractions. The morphological features were semiquantitatively assessed by specific score values. The clinical and morphological correspondence was noted for 35 cases (68.62%), whereas inconsistency was recorded for 16 cases (31.38%). The results of the statistical analysis revealed the correlations between clinically and pathologically diagnosed acute/chronic pulpitides. No significant differences were established between the score values for inflammatory infiltrate intensity, collagen depositions, calcifications and necrosis, and acute, respectively chronic pulpitides. We also obtained significant differences between acute pulpitides and inflammatory infiltrate and calcifications and between chronic pulpitides and inflammatory infiltrate, collagen deposition, and calcifications. On the basis of the predominant pathological aspects, namely, acute and chronic pulpitis, we consider that the classification schemes can be simplified by adequately reducing the number of clinical entities. PMID:26078972

  19. BLEACHING EUCALYPTUS PULPS WITH SHORT SEQUENCES

    Directory of Open Access Journals (Sweden)

    Flaviana Reis Milagres

    2011-03-01

    Full Text Available Eucalyptus spp kraft pulp, due to its high content of hexenuronic acids, is quite easy to bleach. Therefore, investigations have been made attempting to decrease the number of stages in the bleaching process in order to minimize capital costs. This study focused on the evaluation of short ECF (Elemental Chlorine Free and TCF (Totally Chlorine Free sequences for bleaching oxygen delignified Eucalyptus spp kraft pulp to 90% ISO brightness: PMoDP (Molybdenum catalyzed acid peroxide, chlorine dioxide and hydrogen peroxide, PMoD/P (Molybdenum catalyzed acid peroxide, chlorine dioxide and hydrogen peroxide, without washing PMoD(PO (Molybdenum catalyzed acid peroxide, chlorine dioxide and pressurized peroxide, D(EPODP (chlorine dioxide, extraction oxidative with oxygen and peroxide, chlorine dioxide and hydrogen peroxide, PMoQ(PO (Molybdenum catalyzed acid peroxide, DTPA and pressurized peroxide, and XPMoQ(PO (Enzyme, molybdenum catalyzed acid peroxide, DTPA and pressurized peroxide. Uncommon pulp treatments, such as molybdenum catalyzed acid peroxide (PMo and xylanase (X bleaching stages, were used. Among the ECF alternatives, the two-stage PMoD/P sequence proved highly cost-effective without affecting pulp quality in relation to the traditional D(EPODP sequence and produced better quality effluent in relation to the reference. However, a four stage sequence, XPMoQ(PO, was required to achieve full brightness using the TCF technology. This sequence was highly cost-effective although it only produced pulp of acceptable quality.

  20. PRODUCTION OF PRINTING AND WRITING PAPER GRADE PULP FROM ELEPHANT GRASS

    Directory of Open Access Journals (Sweden)

    Marcela Freitas Andrade

    2016-09-01

    Full Text Available The main goal of this study was to characterize chemically and morphologically elephant grass (Pennisetum purpureum, and evaluate the potential of its fibers for production of printing and writing paper grade pulp. The elephant grass was chemically and morphologically characterized and cooked by the soda process to two different degrees of delignification (kappa 17.5 and 10.6. The resulting pulps were fully bleached by the O-D*-(EP-D sequence and characterized for their beatability, drainability and physical-mechanical properties. The lignin content (20.2% was low, indicating that this grass should be easier to pulp. The morphological analyses of the elephant grass indicated a short fiber material, similar to hardwoods. The soda pulp from elephant grass cooked to kappa number 17.5 presented higher screened yield than 10.6 kappa pulp, with alkali demands of 15.0% and 20.0%, respectively. The total active chlorine required by the 17.5 and 10.6 kappa pulps, were 42.1 and 35.1 kg/odt pulp, respectively, to achieve an ISO brightness of approximately 90.0%. The bleached soda pulps cooked to 17.5 and 10.6 kappa number showed similar refinability and resistance to drainage, but the tensile and burst index were highest for the 17.5 kappa pulp at beating energy consumptions in the range of 0-6 Wh. It was concluded that both 10.6 and 17.5 kappa pulps from elephant grass are suitable for the production of printing and writing paper grade pulps, but the highest kappa 17.5 pulp is more economically attractive given its highest pulping yield, despite the significantly increased of chemical demand for bleaching A produção de papel para impressão e escrita Grau de celulose a partir de capim-elefante.

  1. The caries process and its effect on the pulp

    DEFF Research Database (Denmark)

    Bjørndal, Lars

    2008-01-01

    The understanding of the caries process and its effect on the pulp is presented in the context that caries does develop in various rates of progression. Early in the caries process, the pulp reflects changes within lesion activity. Thus, the early pulp response is reversible. Later, the rate...... of caries progression is reflected by the quality of the tertiary dentin. Slowly progressing lesions create tertiary dentin resembling normal tubular dentin. Rapidly progressing lesions lead to the production of a tubular dentin or complete absence of tertiary dentin, as well as pulp necrosis and apical...

  2. Catalase activity in healthy and inflamed pulp tissues of permanent ...

    African Journals Online (AJOL)

    2015-11-02

    Nov 2, 2015 ... pulps, which is due to pulpitis in comparison to healthy dental pulp. Key words: .... human dental pulp cells by Porphyromonas endodontalis lipopolysaccharide. J Endod ... Biology of disease: Free radicals and tissue injury.

  3. Dental Pulp Defence and Repair Mechanisms in Dental Caries.

    Science.gov (United States)

    Farges, Jean-Christophe; Alliot-Licht, Brigitte; Renard, Emmanuelle; Ducret, Maxime; Gaudin, Alexis; Smith, Anthony J; Cooper, Paul R

    2015-01-01

    Dental caries is a chronic infectious disease resulting from the penetration of oral bacteria into the enamel and dentin. Microorganisms subsequently trigger inflammatory responses in the dental pulp. These events can lead to pulp healing if the infection is not too severe following the removal of diseased enamel and dentin tissues and clinical restoration of the tooth. However, chronic inflammation often persists in the pulp despite treatment, inducing permanent loss of normal tissue and reducing innate repair capacities. For complete tooth healing the formation of a reactionary/reparative dentin barrier to distance and protect the pulp from infectious agents and restorative materials is required. Clinical and in vitro experimental data clearly indicate that dentin barrier formation only occurs when pulp inflammation and infection are minimised, thus enabling reestablishment of tissue homeostasis and health. Therefore, promoting the resolution of pulp inflammation may provide a valuable therapeutic opportunity to ensure the sustainability of dental treatments. This paper focusses on key cellular and molecular mechanisms involved in pulp responses to bacteria and in the pulpal transition between caries-induced inflammation and dentinogenic-based repair. We report, using selected examples, different strategies potentially used by odontoblasts and specialized immune cells to combat dentin-invading bacteria in vivo.

  4. Dental Pulp Defence and Repair Mechanisms in Dental Caries

    Directory of Open Access Journals (Sweden)

    Jean-Christophe Farges

    2015-01-01

    Full Text Available Dental caries is a chronic infectious disease resulting from the penetration of oral bacteria into the enamel and dentin. Microorganisms subsequently trigger inflammatory responses in the dental pulp. These events can lead to pulp healing if the infection is not too severe following the removal of diseased enamel and dentin tissues and clinical restoration of the tooth. However, chronic inflammation often persists in the pulp despite treatment, inducing permanent loss of normal tissue and reducing innate repair capacities. For complete tooth healing the formation of a reactionary/reparative dentin barrier to distance and protect the pulp from infectious agents and restorative materials is required. Clinical and in vitro experimental data clearly indicate that dentin barrier formation only occurs when pulp inflammation and infection are minimised, thus enabling reestablishment of tissue homeostasis and health. Therefore, promoting the resolution of pulp inflammation may provide a valuable therapeutic opportunity to ensure the sustainability of dental treatments. This paper focusses on key cellular and molecular mechanisms involved in pulp responses to bacteria and in the pulpal transition between caries-induced inflammation and dentinogenic-based repair. We report, using selected examples, different strategies potentially used by odontoblasts and specialized immune cells to combat dentin-invading bacteria in vivo.

  5. Human tooth pulp anatomy visualization by 3D magnetic resonance microscopy

    International Nuclear Information System (INIS)

    Sustercic, Dusan; Sersa, Igor

    2012-01-01

    Precise assessment of dental pulp anatomy is of an extreme importance for a successful endodontic treatment. As standard radiographs of teeth provide very limited information on dental pulp anatomy, more capable methods are highly appreciated. One of these is 3D magnetic resonance (MR) microscopy of which diagnostic capabilities in terms of a better dental pulp anatomy assessment were evaluated in the study. Twenty extracted human teeth were scanned on a 2.35 T MRI system for MR microscopy using the 3D spin-echo method that enabled image acquisition with isotropic resolution of 100 μm. The 3D images were then post processed by ImageJ program (NIH) to obtain advanced volume rendered views of dental pulps. MR microscopy at 2.35 T provided accurate data on dental pulp anatomy in vitro. The data were presented as a sequence of thin 2D slices through the pulp in various orientations or as volume rendered 3D images reconstructed form arbitrary view-points. Sequential 2D images enabled only an approximate assessment of the pulp, while volume rendered 3D images were more precise in visualization of pulp anatomy and clearly showed pulp diverticles, number of pulp canals and root canal anastomosis. This in vitro study demonstrated that MR microscopy could provide very accurate 3D visualization of dental pulp anatomy. A possible future application of the method in vivo may be of a great importance for the endodontic treatment

  6. Pulp and paper production from Spruce wood with kraft and ...

    African Journals Online (AJOL)

    STORAGESEVER

    2010-03-15

    Mar 15, 2010 ... optical properties of resultant paper were included to determine the properties of these pulp samples. ... pulping compounds in the kraft method are Na2S and .... Scanning electron microscope (SEM) measurement of pulp.

  7. Scaffold-free Prevascularized Microtissue Spheroids for Pulp Regeneration.

    Science.gov (United States)

    Dissanayaka, W L; Zhu, L; Hargreaves, K M; Jin, L; Zhang, C

    2014-12-01

    Creating an optimal microenvironment that mimics the extracellular matrix (ECM) of natural pulp and securing an adequate blood supply for the survival of cell transplants are major hurdles that need to be overcome in dental pulp regeneration. However, many currently available scaffolds fail to mimic essential functions of natural ECM. The present study investigated a novel approach involving the use of scaffold-free microtissue spheroids of dental pulp stem cells (DPSCs) prevascularized by human umbilical vein endothelial cells (HUVECs) in pulp regeneration. In vitro-fabricated microtissue spheroids were inserted into the canal space of tooth-root slices and were implanted subcutaneously into immunodeficient mice. Histological examination revealed that, after four-week implantation, tooth-root slices containing microtissue spheroids resulted in well-vascularized and cellular pulp-like tissues, compared with empty tooth-root slices, which were filled with only subcutaneous fat tissue. Immunohistochemical staining indicated that the tissue found in the tooth-root slices was of human origin, as characterized by the expression of human mitochondria, and contained odontoblast-like cells organized along the dentin, as assessed by immunostaining for nestin and dentin sialoprotein (DSP). Vascular structures formed by HUVECs in vitro were successfully anastomosed with the host vasculature upon transplantation in vivo, as shown by immunostaining for human CD31. Collectively, these findings demonstrate that prevascularized, scaffold-free, microtissue spheroids can successfully regenerate vascular dental pulp-like tissue and also highlight the significance of the microtissue microenvironment as an optimal environment for successful pulp-regeneration strategies. © International & American Associations for Dental Research.

  8. Pulp and paper from oil palm fronds: Wavelet neural networks modeling of soda-ethanol pulping

    OpenAIRE

    Zarita Zainuddin; Wan Rosli Wan Daud; Pauline Ong; Amran Shafie

    2012-01-01

    Wavelet neural networks (WNNs) were used to investigate the influence of operational variables in the soda-ethanol pulping of oil palm fronds (viz. NaOH concentration (10-30%), ethanol concentration (15-75%), cooking temperature (150-190 ºC), and time (60-180 min)) on the resulting pulp and paper properties (viz. screened yield, kappa number, tensile index, and tear index). Performance assessments demonstrated the predictive capability of WNNs, in that the experimental results of the dependen...

  9. CD146 positive human dental pulp stem cells promote regeneration of dentin/pulp-like structures.

    Science.gov (United States)

    Matsui, Mikiko; Kobayashi, Tomoko; Tsutsui, Takeo W

    2018-04-01

    CD146 and STRO-1 are endothelial biomarkers that are co-expressed on the cellular membranes of blood vessels within human dental pulp tissue. This study characterized the percentage of dentin-like structures produced by CD146-positive (CD146 + ) human dental pulp stem cells (DPSCs), compared with their CD146-negative (CD146 - ) counterparts. DPSC populations were enriched using magnetic-activated cell sorting (MACS), yielding CD146 + and CD146 - cells, as well as mixtures composed of 25% CD146 + cells and 75% CD146 - cells (CD146 +/- ). Cell growth assays indicated that CD146 + cells exhibit an approximate 3-4 h difference in doubling time, compared with CD146 - cells. Cell cycle distributions were determined by flow cytometry analysis. The low percentage of CD146 + cells' DNA content in G 0 /G 1 phase were compared with CD146 - and non-separated cells. In contrast to CD146 - and non-separated cells, prompt mineralization was observed in CD146 + cells. Subsequently, qRT-PCR revealed high mRNA expression of CD146 and Alkaline phosphatase in mineralization-induced CD146 + cells. CD146 + cells were also observed high adipogenic ability by Oil red O staining. Histological examinations revealed an increased area of dentin/pulp-like structures in transplanted CD146 + cells, compared with CD146 - and CD146 +/- cells. Immunohistochemical studies detected dentin matrix protein-1 (DMP1) and dentin sialophosphoprotein (DSPP), as well as human mitochondria, in transplanted DPSCs. Co-expression of CD146 and GFP indicated that CD146 was expressed in transplanted CD146 + cells. CD146 + cells may promote mineralization and generate dentin/pulp-like structures, suggesting a role in self-renewal of stem cells and dental pulp regenerative therapy.

  10. Characterization of sugar beet pulp derived oligosaccharides

    NARCIS (Netherlands)

    Leijdekkers, M.

    2015-01-01

    Abstract

    This thesis aimed at characterizing complex mixtures of sugar beet pulp derived oligosaccharides, in order to be able to monitor and optimize the enzymatic saccharification of sugar beet pulp.

    Hydrophilic interaction chromatography with on-line evaporative

  11. Influence of moderate to severe chronic periodontitis on dental pulp.

    Science.gov (United States)

    Fatemi, K; Disfani, R; Zare, R; Moeintaghavi, A; Ali, Saadat A; Boostani, H R

    2012-10-01

    The relationship between periodontal disease and dental pulp changes is controversial and has been debated for many years. This human study was performed to evaluate the possible effects of moderate to advanced periodontal disease on the different aspect of dental pulp structure. Twenty hopeless permanent teeth were extracted from systemically healthy adults because of moderate to advanced chronic periodontitis, with a bone loss of >6 mm and a mobility of grade 2 or 3. Upon extraction, the apical 2 to 3 mm of the roots were immediately sectioned. Four to five sections were mounted on each slide, and every third slide was stained with hematoxylin and eosin. The specimens were histologically processed and examined by an oral pathologist. Non-inflamed pulp, with partial or complete necrosis in some sections and several non-necrotic sections, was found in only 6.3% of teeth. Most teeth (58.3%) displayed edematous pulps. Slightly fibrotic pulps were seen in 52.1% of sections. Odontoblastic integrity was seen in 31.3% of teeth. Most teeth (77.1%) displayed no pulp stones. In 43.8% of teeth, the pulp vessels displayed dilatation. Moderate to advanced periodontal disease can affect the dental pulp. Careful consideration of diagnostic and treatment planing in patients with endodontic-periodontal involvement is therefore recommended.

  12. Dental pulp stem cells in regenerative dentistry.

    Science.gov (United States)

    Casagrande, Luciano; Cordeiro, Mabel M; Nör, Silvia A; Nör, Jacques E

    2011-01-01

    Stem cells constitute the source of differentiated cells for the generation of tissues during development, and for regeneration of tissues that are diseased or injured postnatally. In recent years, stem cell research has grown exponentially owing to the recognition that stem cell-based therapies have the potential to improve the life of patients with conditions that span from Alzheimer's disease to cardiac ischemia to bone or tooth loss. Growing evidence demonstrates that stem cells are primarily found in niches and that certain tissues contain more stem cells than others. Among these tissues, the dental pulp is considered a rich source of mesenchymal stem cells that are suitable for tissue engineering applications. It is known that dental pulp stem cells have the potential to differentiate into several cell types, including odontoblasts, neural progenitors, osteoblasts, chondrocytes, and adipocytes. The dental pulp stem cells are highly proliferative. This characteristic facilitates ex vivo expansion and enhances the translational potential of these cells. Notably, the dental pulp is arguably the most accessible source of postnatal stem cells. Collectively, the multipotency, high proliferation rates, and accessibility make the dental pulp an attractive source of mesenchymal stem cells for tissue regeneration. This review discusses fundamental concepts of stem cell biology and tissue engineering within the context of regenerative dentistry.

  13. The impact of kappa number composition on eucalyptus kraft pulp bleachability

    Directory of Open Access Journals (Sweden)

    M. M. Costa

    2007-03-01

    Full Text Available Consumption of chemicals during ECF bleaching of kraft pulp correlates reasonably well with kappa number, which measures with KMnO4 the total amount of oxidizable material in the pulp. However, the method does not distinguish between the oxidizable material in residual lignin and other structures susceptible to oxidation, such as hexenuronic acids (HexAs, extractives and carbonyl groups in the pulp. In this study an attempt is made to separate the main contributors to the kappa number in oxygen - delignified eucalyptus Kraft pulps and evaluate how these fractions behave during ECF bleaching using chlorine dioxide as the sole oxidant (DEDD sequence. Residual lignin and HexAs proved to be the main fractions contributing to the kappa number and chlorine dioxide consumption in ECF bleaching. Pulp bleachability with chlorine dioxide increases with increasing HexAs content of the pulp but chlorine dioxide per se does not react with HexAs. Reduction of pulp with sodium borohydride under conditions for removing carbonyl groups has no impact on bleachability. No correlation was found between the pulp of the extractive content and pulp bleachability. The removal of HexAs prior to ECF bleaching significantly decreases the formation of chlorinated organics in the pulp (OX and filtrates (AOX as well as of oxalic acids in the filtrates.

  14. Isolation and characterization of lignocellulose nanofibers from different wheat straw pulps.

    Science.gov (United States)

    Sánchez, Rafael; Espinosa, Eduardo; Domínguez-Robles, Juan; Loaiza, Javier Mauricio; Rodríguez, Alejandro

    2016-11-01

    Wheat straw was cooked under different pulping processes: Soda (100°C, 7% NaOH, 150min), Kraft (170°C, 16% alkalinity, 25% sulfidity, 40min) and Organosolv (210°C, 60% ethanol, 60min). Once the pulps were obtained, lignocellulose nanofibers (LCNF) were isolated by mechanical process and TEMPO-mediated oxidation followed by a high pressure homogenization. After pulping process, the different pulps were characterized and its chemical composition was determined. The pulps characterization indicates that the Soda process is the process that, despite producing less delignification, retains much of the hemicelluloses in the pulp, being this content a key factor in the nanofibrillation process. Regarding the LCNF obtained by mechanical process, those nanofibers isolated from Organosolv wheat pulp (OWP) and Kraft wheat pulp (KWP) show low values for nanofibrillation yield, specific surface area and greater diameter. However, those nanofibers isolated from Soda wheat pulp (SWP) reach much higher values for these parameters and presents a diameter of 14nm, smaller than those obtained by TEMPO-mediated oxidation from OWP. Smaller diameters are generally obtained in TEMPO-oxidized LCNF. This work concludes that the lignin content does not affect greatly to obtain LCNF as does the hemicellulose content, so it is accurate to use a soft pulping process. Copyright © 2016 Elsevier B.V. All rights reserved.

  15. Convection flow structure in the central polar cap

    Science.gov (United States)

    Bristow, W. A.

    2017-12-01

    A previous study of spatially averaged flow velocity in the central polar cap [Bristow et al., 2015] observed under steady IMF conditions found that it was extremely rare for the average to exceed 850 m/s (less than 0.2 % of the time). Anecdotally, however it is not uncommon to observe line-of-sight velocities in excess of 100 m/s in the McMurdo radar field of view directly over the magnetic pole. This discrepancy motivated this study, which examines the conditions under which high-velocity flows are observed at latitudes greater than 80° magnetic latitude. It was found that highly structured flows are common in the central polar cap, which leads to the flow within regions to have significant deviation from the average. In addition, the high-speed flow regions are usually directed away from the earth-sun line. No specific set of driving conditions was identified to be associated with high-speed flows. The study did conclude that 1)Polar cap velocities are generally highly structured. 2)Flow patterns typically illustrate narrow channels, vortical flow regions, and propagating features. 3) Persistent waves are a regular occurrence. 3)Features are observed to propagate from day side to night side, and from night side to day side.. 4)Convection often exhibits significant difference between the two hemispheres. And 5)About 10% of the time the velocity somewhere in the cap exceeds 1 Km/s The presentation will conclude with a discussion of the physical reasons for the flow structure. Bristow, W. A., E. Amata, J. Spaleta, and M. F. Marcucci (2015), Observations of the relationship between ionospheric central polar cap and dayside throat convection velocities, and solar wind/IMF driving, J. Geophys. Res. Space Physics, 120, doi:10.1002/2015JA021199.

  16. Properties of Enzyme Pretreated Wikstroemia sikokiana and Broussonetia papyrifera Bast Fiber Pulps

    Directory of Open Access Journals (Sweden)

    Lang-Dong Lin

    2015-04-01

    Full Text Available Xylanase, pectinase complex, and BL11 pectinase were employed for the pretreatment of gampi and paper mulberry bast fiber pulps prior to chlorine dioxide bleaching. The bleaching efficiencies of the pulps with different enzymatic pretreatments were investigated. Accelerated aging by heat-humidity treatment was also conducted to evaluate yellowing phenomena and to estimate the prevention of brightness reversion (brightness retention by enzymatic pretreatment. The order of active chlorine required with respect to pretreatment was pectinase complex > xylanase > BL11 pectinase for soda and soda/oxalate gampi pulps and pectinase complex > BL11 pectinase > xylanase for soda and soda/oxalate paper mulberry pulps. Higher brightness retention values were observed for soda/oxalate pulps compared to soda pulps. The brightness retention levels for gampi pulps and mulberry pulps after ClO2 bleaching with enzymatic pretreatment were higher than the levels of ClO2 and NaClO bleaching pulps. Enzymatic treatments were thus able to reduce the usage of ClO2 and to assist in producing photo-stable paper materials for art and artifact-repairing applications. Thus, enzymatic pretreatment of the pulp has the potential to meet world trends and environmental sustainability for pulp and paper industries.

  17. Extraction of hemicelluloses from wood in a pulp biorefinery, and subsequent fermentation into ethanol

    International Nuclear Information System (INIS)

    Boucher, Jérémy; Chirat, Christine; Lachenal, Dominique

    2014-01-01

    Highlights: • Hemicellulosic ethanol from softwood hemicelluloses in a pulp mill. • Comparison of acid hydrolysis and autohydrolysis to extract hemicelluloses. • Effects of the extraction process conditions on inhibitors concentrations. • Effects of inhibitors on fermentation. - Abstract: This study deals with the production of ethanol and paper pulp in a kraft pulp mill. The use of an acid hydrolysis or a two-step treatment composed of an autohydrolysis followed by a secondary acid hydrolysis was studied. Acid hydrolysis allowed the extraction of higher quantities of sugars but led also to higher degradations of these sugars into inhibitors of fermentation. The direct fermentation of a hydrolysate resulting from an acid hydrolysis gave excellent yields after 24 h. However, the fermentation of hydrolysates after their concentration proved to be impossible. The study of the impact of the inhibitors on the fermentations showed that organic acids, and more specifically formic acid and acetic acid were greatly involved in the inhibition

  18. Fungal biomass production from coffee pulp juice

    Energy Technology Data Exchange (ETDEWEB)

    De Leon, R.; Calzada, F.; Herrera, R.; Rolz, C.

    1980-01-01

    Coffee pulp or skin represents about 40% of the weight of the fresh coffee fruit. It is currently a waste and its improper handling creates serious pollution problems for coffee producing countries. Mechanical pressing of the pulp will produce two fractions: coffee pulp juice (CPJ) and pressed pulp. Aspergillus oryzae, Trichoderma harzianum, Penicillium crustosum and Gliocladium deliquescens grew well in supplemented CPJ. At shake flask level the optimum initial C/N ratio was found to be in the range of 8 to 14. At this scale, biomass values of up to 50 g/l were obtained in 24 hours. Biomass production and total sugar consumption were not significantly different to all fungal species tested at the bench-scale level, even when the initial C/N ratio was varied. Best nitrogen consumption values were obtained when the initial C/N ratio was 12. Maximum specific growth rates occurred between 4-12 hours for all fungal species tested. (Refs. 8).

  19. TESTING OF PULP VITALITY BY PULSOXIMETRY

    Directory of Open Access Journals (Sweden)

    Gabriela CIOBANU

    2012-06-01

    Full Text Available The methods applied for diagnosing the health condition of the pulp tissue are numerous, however, nowadays, an increasingly higher number of conventional tests are replaced by some objective, non-invasive, painless and reliable tests. Among them, pulse oximetry is a method for the investigation of pulp vitality based on oxygen saturation (SaO2 of the hemoglobin from the blood present in the pulp vascular bed, as a means of differentiating among the vital and the non-vital teeth. In the present study, registrations were made on a group of 120 frontal maxillary teeth, in patients with ages between 20 and 40 years, on using a digital sensor modified by the pulse oximeter with which the pulse and the values of oxygen saturation were measured at the level of both teeth and right hand finger. The mean SaO2 value in the pulp blood of the vital teeth was of 83.30% for the central incisor, of 78.51% for the lateral one and of 84.56%, respectively, for the canine; the value recorded at finger level was of 97%. In the non-vital teeth, the SaO2 value measured on the pulse oximeter was of 0%. Pulse registration showed mean values of 70.56 beatings/min at tooth level and of 70.88 beatings/min, respectively, at finger level. The results of the present study may confirm that pulse oximetry represents a simple, non-traumatic, efficient and objective method for testing the vitality condition of the dental pulp.

  20. Performance of a Horizontal Triple Cylinder Type Pulping Machine

    Directory of Open Access Journals (Sweden)

    Sukrisno Widyotomo

    2011-05-01

    Full Text Available Pulping is one important step in wet coffee processing method. Pulping process usually uses a machine which constructed by wood or metal materials. A horizontal single cylinder type of fresh coffee cherries pulping machine is the most popular machine in coffee processing. One of the weaknesses of a horizontal single cylinder type of fresh coffee cherries pulping machine is higher in broken beans. Broken bean is one of mayor aspects in defect system that contribute to low quality. Indonesian Coffee and Cocoa Research Institute has designed and tested a horizontal double cylinder type of fresh coffee cherries pulping machine which resulted in 12.6—21.4% of broken beans. To reduce percentage of broken beans, Indonesian Coffee and Cocoa Research Institute has developed and tested a horizontal triple cylinder type of fresh coffee cherries pulping machine. Material tested was fresh mature Robusta coffee cherries, 60—65% (wet basis moisture content; has classified on 3 levels i.e. unsorted, small and medium, and clean from metal and foreign materials. The result showed that the machine produced 6,340 kg/h in optimal capacity for operational conditions, 1400 rpm rotor rotation speed for unsorted coffee cherries with composition 55.5% whole parchment coffee, 3.66% broken beans, and 1% beans in wet skin.Key words : coffee, pulp, pulper, cylinder, quality.

  1. Prospect of trema orientalis as a pulping raw material in bangladesh

    International Nuclear Information System (INIS)

    Jahan, M.S.

    2013-01-01

    The pulp and paper industry in Bangladesh is looking for new raw materials with high productivity per hectare. Currently, bamboo and Gmelina arborea are the main pulping raw materials for the pulp and paper industry of the country. Trema orientalis is one of the fastest growing woods in the tropical region. This paper reviews the literature on the topic, in particular, related to the chemical, morphological and physical properties of T. orientalis and its suitability for pulping. In addition, the advantages and disadvantages of various ulping processes proposed in the literature were critically analyzed. It was concluded that T. orientalis is a potential pulp wood for the Bangladesh pulp and paper industry. (author)

  2. Factors affecting the optimal performance of a high-yield pulping operation

    Energy Technology Data Exchange (ETDEWEB)

    Broderick, G [Noranda Technology Centre, Pointe-Claire, PQ (Canada); Paris, J [Ecole Polytechnique, Montreal, PQ (Canada); Valada, J L [Quebec Univ., Trois-Rivieres, PQ (Canada)

    1995-06-01

    Strategies for operating a chemical-mechanical pulp mill were investigated from data based on process models from some one hundred pilot scale pulping runs. Optimal values for 55 process and pulp quality variables have been calculated by applying a genetic algorithm search to a fuzzy model of the overall system. Best pulp quality was achieved and maintained when the chemical pretreatment was conducted at moderately low temperatures using a high SO{sub 2} concentration, which produced high sulphonation and high yield at the same time. By characterizing the quality of the pulp at the fibre level, optimization results were said to be more easily transferable to other high yield pulping systems. 19 refs., 6 tabs.

  3. De-pulping and Seed Separation from Tumba ( Citrullus colocynthis) Fruit

    Science.gov (United States)

    Mudgal, Vishvambhar Dayal

    2017-09-01

    Tumba ( Citrullus colocynthis) contains spongy pulp in which seeds are embedded unevenly. Seeds contain about 26% fats and 13% protein. The process of seed separation is highly time consuming and labour intensive. Two weeks are required to separate its seeds with traditional methods. The developed prototype, for separating tumba seeds, mainly consists of chopper, de-pulping screw, barrel assembly and seed separation unit. The de-pulping screw and barrel assembly was divided in two sections i.e. conveying (feeding zone) and compression sections (de-pulping zone). The performance of developed machine was evaluated at different screw speed in the range of 40-100 rpm. Maximum pulp removal efficiency of 78.1% was achieved with screw speed of 60 rpm. Seed separation from the pulp was carried out by adding different chemicals. Use of sodium hydroxide and potassium hydroxide produced seed separation up to 99%.

  4. Comparative pulping of sunflower stalks

    Directory of Open Access Journals (Sweden)

    Valerii Barbash

    2016-03-01

    Full Text Available The procedure of holocellulose content determination in non-wood plant raw materials was developed. The strength properties of pulp obtained from sunflower stalks by neutral-sulphite, soda, alkaline sulphite-anthraquinone-ethanol and peracetic methods of delignification were studied. Methodology of comparison of plant materials delignification methods using new lignin-carbohydrate diagram was proposed. It was shown, that the alkaline sulphite-anthraquinone-ethanol method of pulping is characterized by the highest delignification degree and is the most efficient among the studied methods

  5. The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity.

    Science.gov (United States)

    Ogino, Minako; Ito, Naoto; Sugiyama, Makoto; Ogino, Tomoaki

    2016-05-21

    The large (L) protein of rabies virus (RABV) plays multiple enzymatic roles in viral RNA synthesis and processing. However, none of its putative enzymatic activities have been directly demonstrated in vitro. In this study, we expressed and purified a recombinant form of the RABV L protein and verified its guanosine 5'-triphosphatase and GDP polyribonucleotidyltransferase (PRNTase) activities, which are essential for viral mRNA cap formation by the unconventional mechanism. The RABV L protein capped 5'-triphosphorylated but not 5'-diphosphorylated RABV mRNA-start sequences, 5'-AACA(C/U), with GDP to generate the 5'-terminal cap structure G(5')ppp(5')A. The 5'-AAC sequence in the substrate RNAs was found to be strictly essential for RNA capping with the RABV L protein. Furthermore, site-directed mutagenesis showed that some conserved amino acid residues (G1112, T1170, W1201, H1241, R1242, F1285, and Q1286) in the PRNTase motifs A to E of the RABV L protein are required for cap formation. These findings suggest that the putative PRNTase domain in the RABV L protein catalyzes the rhabdovirus-specific capping reaction involving covalent catalysis of the pRNA transfer to GDP, thus offering this domain as a target for developing anti-viral agents.

  6. To Study Capping or Lamination Tendency of Tablets Through Evaluation of Powder Rheological Properties and Tablet Mechanical Properties of Directly Compressible Blends.

    Science.gov (United States)

    Dudhat, Siddhi M; Kettler, Charles N; Dave, Rutesh H

    2017-05-01

    Air entrapment efficiency of the powders is one of the main factors leading to occurrence of capping or lamination tendency of tablets manufactured from the directly compressible powder blends. The purpose of the current research was to study this underlying cause leading to occurrence of capping or lamination of tablets through evaluation of powder rheological properties. Powder blends were prepared by addition of 0% w/w to 100% w/w of individual active pharmaceutical ingredient (API) [two model API: acetaminophen (APAP) and ibuprofen (IBU)] with microcrystalline cellulose without and with 0.5% w/w Magnesium Stearate as lubricant. Powder rheological properties were analyzed using FT4 Powder Rheometer for dynamic, bulk, and shear properties. Tablet mechanical properties of the respective blends were studied by determining the ability of the material to form tablet of specific strength under applied compaction pressure through tabletability profile. The results showed that powder rheometer distinguished the powder blends based on their ability to relieve entrapped air along with the distinctive flow characteristics. Powder blend prepared with increasing addition of APAP displayed low powder permeability as compared to IBU blends with better powder permeability, compressibility and flow characteristics. Also, lubrication of the APAP blends did not ease their ability to relieve air. Tabletability profiles revealed the potential occurrence of capping or lamination in tablets prepared from the powder blends with high APAP content. This study can help scientist to understand tableting performance at the early-developmental stages and can avoid occurrence capping and lamination of tablets.

  7. Proteomic analysis of human tooth pulp: proteomics of human tooth.

    Science.gov (United States)

    Eckhardt, Adam; Jágr, Michal; Pataridis, Statis; Mikšík, Ivan

    2014-12-01

    The unique pulp-dentin complex demonstrates strong regenerative potential, which enables it to respond to disease and traumatic injury. Identifying the proteins of the pulp-dentin complex is crucial to understanding the mechanisms of regeneration, tissue calcification, defense processes, and the reparation of dentin by dental pulp. The lack of knowledge of these proteins limits the development of more efficient therapies. The proteomic profile of human tooth pulp was investigated and compared with the proteome of human dentin and blood. The samples of tooth pulp were obtained from 5 sound permanent human third molars of 5 adults (n = 5). The extracted proteins were separated by 2-dimensional gel electrophoresis, analyzed by nano-liquid chromatography tandem mass spectrometry, and identified by correlating mass spectra to the proteomic databases. A total of 342 proteins were identified with high confidence, and 2 proteins were detected for the first time in an actual human sample. The identified tooth pulp proteins have a variety of functions: structural, catalytic, transporter, protease activity, immune response, and many others. In a comparison with dentin and blood plasma, 140 (pulp/dentin) shared proteins were identified, 37 of which were not observed in plasma. It can be suggested that they might participate in the unique pulp-dentin complex. This proteomic investigation of human tooth pulp, together with the previously published study of human dentin, is one of the most comprehensive proteome lists of human teeth to date. Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  8. Chemi-thermomechanical pulping of para rubber waste wood

    Energy Technology Data Exchange (ETDEWEB)

    Kubo, T.; Hosokawa, J.; Kobayashi, T.; Kobayashi, Y.

    1981-01-01

    The addition of NaOH to Na/sub 2/SO/sub 3/-treated waste wood chips (Hevea brasiliensis) increased long fiber fraction, Klason lignin content and bulk density, improved breaking length and tear factor, and decreased energy consumption in refining and brightness of resulting chemithermomechanical pulp. Lowering in brightness by alkali treatment was recovered by H/sub 2/O/sub 2/ bleaching, and bleaching with 8% H/sub 2/O/sub 2/ on pulp gave pulp with 61.3% brightness.

  9. Influence of moderate to severe chronic periodontitis on dental pulp

    Science.gov (United States)

    Fatemi, K; Disfani, R; Zare, R; Moeintaghavi, A; Ali, Saadat A.; Boostani, H. R

    2012-01-01

    Background: The relationship between periodontal disease and dental pulp changes is controversial and has been debated for many years. This human study was performed to evaluate the possible effects of moderate to advanced periodontal disease on the different aspect of dental pulp structure. Materials and Methods: Twenty hopeless permanent teeth were extracted from systemically healthy adults because of moderate to advanced chronic periodontitis, with a bone loss of >6 mm and a mobility of grade 2 or 3. Upon extraction, the apical 2 to 3 mm of the roots were immediately sectioned. Four to five sections were mounted on each slide, and every third slide was stained with hematoxylin and eosin. The specimens were histologically processed and examined by an oral pathologist. Results: Non-inflamed pulp, with partial or complete necrosis in some sections and several non-necrotic sections, was found in only 6.3% of teeth. Most teeth (58.3%) displayed edematous pulps. Slightly fibrotic pulps were seen in 52.1% of sections. Odontoblastic integrity was seen in 31.3% of teeth. Most teeth (77.1%) displayed no pulp stones. In 43.8% of teeth, the pulp vessels displayed dilatation. Conclusions: Moderate to advanced periodontal disease can affect the dental pulp. Careful consideration of diagnostic and treatment planing in patients with endodontic-periodontal involvement is therefore recommended. PMID:23493524

  10. CAP Reform and the Doha Development Agenda

    NARCIS (Netherlands)

    van Dijck, P.; Faber, G.

    2004-01-01

    The CAP reforms that the EU accepted in June 2003 will partially decouple direct income payments to farmers from production and make these payments conditional on cross-compliance. The reforms are driven by enlargement of EU membership, budgetary constraints, mounting pressures from diverse animal

  11. Seeking optimality in fruit pulping schedules: A case study*

    Directory of Open Access Journals (Sweden)

    J.H. Van Vuuren

    2014-01-01

    Full Text Available The process of scheduling fruit pulping for the production of fruit juices is of great importance in the beverage industry. Decisions have to be made regarding available processing time, the disposal of fruit that will not be pulped before stock loss due to spoilage, the fulfilment of customer demand and an optimal financial position. Sheduling depends on the capacity of the work force, pulping machine limitations and delivery deadlines. However, the situation is often encountered where the plant manager has to decide which fruit batches (usually from stock piles of overwhelming proportions during the harvesting season are to be pulped in order to minimize losses due to fruit deterioration. Such decisions are usually done manually, based on intuition and experience. A mathematical model is presented here which constructs a pulping strategy while minimising cascading financial losses associated with fruit grade drops within the stock pile. It is shown in particular that a minimisation of fruit losses is not a good criterion for optimality, and that substantial financial gains may be accomplished when minimising financial losses in stead of fruit losses, which is currently standard practice at most fruit pulping plants.

  12. Direct visualization of redistribution and capping of fluorescent gangliosides on lymphocytes

    OpenAIRE

    1984-01-01

    Fluorescent derivatives of gangliosides were prepared by oxidizing the sialyl residues to aldehydes and reacting them with fluorescent hydrazides. When rhodaminyl gangliosides were incubated with lymphocytes, the cells incorporated them in a time- and temperature- dependent manner. Initially, the gangliosides were evenly distributed on the cell surface but were redistributed into patches and caps by antirhodamine antibodies. When the cells were then stained with a second antibody or protein A...

  13. Citrate-Induced Nanocubes: A Re-Examination of the Role of Citrate as a Shape-Directing Capping Agent for Ag-Based Nanostructures.

    Science.gov (United States)

    Hajfathalian, Maryam; Gilroy, Kyle D; Hughes, Robert A; Neretina, Svetlana

    2016-07-01

    Seed-mediated syntheses utilizing facet-selective surface passivation provide the necessary chemical controls to direct noble metal nanostructure formation to a predetermined geometry. The foremost protocol for the synthesis of (111)-faceted Ag octahedra involves the reduction of metal ions onto pre-existing seeds in the presence of citrate and ascorbic acid. It is generally accepted that the capping of (111) facets with citrate dictates the shape while ascorbic acid acts solely as the reducing agent. Herein, a citrate-based synthesis is demonstrated in which the presence or absence of ascorbic acid is the shape-determining factor. Reactions are carried out in which Ag(+) ions are reduced onto substrate-immobilized Ag, Au, Pd, and Pt seeds. Syntheses lacking ascorbic acid, in which citrate acts as both the capping and the reducing agent, result in a robust nanocube growth mode able to withstand wide variations in the concentration of reactants, reaction rates, seed material, seed orientation and faceting, pH, and substrate material. If, however, ascorbic acid is included in these syntheses, then the growth mode reverts to one that advances the octahedral geometry. The implication of these results is that citrate, or one of its oxidation products, selectively caps (100) facets, but where this capability is compromised by ascorbic acid. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Isolation and characterization of pulp from sugarcane bagasse and rice straw

    International Nuclear Information System (INIS)

    Saiful Azhari, S.; Suhardy, D.; Kasim, F.H; Nazry Saleh, M.

    2007-01-01

    The amount of sugarcane bagasse and rice straw in the state of Perlis (Malaysia) is abundant while its utilization is still limited. One of the alternatives for the bagasse and straw utilization is as pulp raw material. This paper reviews on pulp from sugarcane bagasse and rice straw and its suitability for paper production. In this study, the pulp was extracted by the Soxhlet extraction method. The objective of this study was to investigate the cellulose, lignin and silica content of the pulp from sugarcane bagasse and rice straw. For rice straw, the presence of large amount of pentosanes in the pulp and black liquors, which also contain silica were decreased the using of straw in the paper industry. Therefore, formic acid pulping and NaOH treatment are studied to reduce or prevent silica. The isolated pulp samples were further characterized by Scanning Electron Microscope (SEM) to investigate their fiber dimensions. (Author)

  15. Relationship between lignin structure and delignification degree in Pinus pinaster kraft pulps.

    Science.gov (United States)

    Baptista, C; Robert, D; Duarte, A P

    2008-05-01

    This study examines the structure of residual and dissolved lignins from Pinus pinaster pulps obtained at different degrees of delignification by laboratory conventional kraft pulping. The cooking H factor was varied from 85 to 8049. The residual and dissolved lignin samples were characterised by elemental analysis, residual carbohydrate content, permanganate oxidation and 13C NMR spectroscopy. The reflectance factor of the pulps was also determined in order to tentatively correlate the delignification degree and residual lignin structure with the pulp colour. The obtained results confirmed that the delignification degree increases the condensation of the lignin structure, which might have an influence upon the observed increased pulp colour. The lack of selectivity of kraft pulping process in the case of more delignified pulps was also shown.

  16. 76 FR 13515 - Medicare Program; Revisions to the Reductions and Increases to Hospitals' FTE Resident Caps for...

    Science.gov (United States)

    2011-03-14

    ...' FTE Resident Caps for Graduate Medical Education Payment Purposes AGENCY: Centers for Medicare... education affiliated groups for the purpose of determining possible full-time equivalent resident cap... caps for direct GME under Medicare for certain hospitals, and to authorize the ``redistribution'' of...

  17. Avoiding total reduced sulfur (TRS) emissions from sodium sulfite pulping recovery processes

    International Nuclear Information System (INIS)

    Norman, J.C.; Sell, N.J.; Ciriacks, J.C.

    1990-01-01

    This paper reports that one of the current trends in paper-making with cellulose pulping is the use of high-yield processes. With yields greater than 65%, these processes include mechanical pulps (groundwood and thermomechanical pulps or TMP), and semichemical types (chemi-TMP or CTMP). Groundwood and TMP make up about 10% of North American pulp production. Semichemical pulp makes up about 7% and is mostly used for corrugating medium. High-yield pulping for linerboard, particularly using the alkaline sulfite process, is also likely to be used in the future. High-yield pulping is based primarily on the sulfite process using mostly sodium-based chemicals. A disadvantage of this process is the unavailability of a recovery system for the inorganic pulping chemicals. Generally, mills have not accepted any particular recovery system for this process. For this and other reasons, sulfite processes constitute only 3-4% of the total North American pulp production. If high-yield processes continue to increase in popularity, a sodium sulfite chemical recovery system will be needed. A number of chemical recovery systems have been developed in the past 30 years for sodium-based sulfite pulping processes, with most of the mills successfully using this process located in Scandinavia

  18. Microarray evaluation of gene expression profiles in inflamed and healthy human dental pulp: the role of IL1beta and CD40 in pulp inflammation.

    Science.gov (United States)

    Gatta, V; Zizzari, V L; Dd ' Amico, V; Salini, L; D' Aurora, M; Franchi, S; Antonucci, I; Sberna, M T; Gherlone, E; Stuppia, L; Tetè, S

    2012-01-01

    Dental pulp undergoes a number of changes passing from healthy status to inflammation due to deep decay. These changes are regulated by several genes resulting differently expressed in inflamed and healthy dental pulp, and the knowledge of the processes underlying this differential expression is of great relevance in the identification of the pathogenesis of the disease. In this study, the gene expression profile of inflamed and healthy dental pulps were compared by microarray analysis, and data obtained were analyzed by Ingenuity Pathway Analysis (IPA) software. This analysis allows to focus on a variety of genes, typically expressed in inflamed tissues. The comparison analysis showed an increased expression of several genes in inflamed pulp, among which IL1β and CD40 resulted of particular interest. These results indicate that gene expression profile of human dental pulp in different physiological and pathological conditions may become an useful tool for improving our knowledge about processes regulating pulp inflammation.

  19. A modified efficient method for dental pulp stem cell isolation

    Directory of Open Access Journals (Sweden)

    Maryam Raoof

    2014-01-01

    Full Text Available Background: Dental pulp stem cells can be used in regenerative endodontic therapy. The aim of this study was to introduce an efficient method for dental pulp stem cells isolation. Materials and Methods: In this in-vitro study, 60 extracted human third molars were split and pulp tissue was extracted. Dental pulp stem cells were isolated by the following three different methods: (1 digestion of pulp by collagenase/dispase enzyme and culture of the released cells; (2 outgrowth of the cells by culture of undigested pulp pieces; (3 digestion of pulp tissue pieces and fixing them. The cells were cultured in minimum essential medium alpha modification (αMEM medium supplemented with 20% fetal bovine serum(FBS in humid 37°C incubator with 5% CO 2 . The markers of stem cells were studied by reverse transcriptase polymerase chain reaction (PCR. The student t-test was used for comparing the means of independent groups. P <0.05 was considered as significant. Results: The results indicated that by the first method a few cell colonies with homogenous morphology were detectable after 4 days, while in the outgrowth method more time was needed (10-12 days to allow sufficient numbers of heterogeneous phenotype stem cells to migrate out of tissue. Interestingly, with the improved third method, we obtained stem cells successfully with about 60% efficiency after 2 days. The results of RT-PCR suggested the expression of Nanog, Oct-4, and Nucleostemin markers in the isolated cells from dental pulps. Conclusion: This study proposes a new method with high efficacy to obtain dental pulp stem cells in a short time.

  20. Challenges of stem cell-based pulp and dentin regeneration: a clinical perspective.

    Science.gov (United States)

    Huang, George T-J; Al-Habib, Mey; Gauthier, Philippe

    2013-03-01

    There are two types of approaches to regenerate tissues: cell-based and cell-free. The former approach is to introduce exogenous cells into the host to regenerate tissues, and the latter is to use materials other than cells in an attempt to regenerate tissues. There has been a significant advancement in stem cell-based pulp and dentin regeneration research in the past few years. Studies in small and large animals have demonstrated that pulp/dentin-like tissues can be regenerated partially or completely in the root canal space with apical openings of 0.7-3.0 mm using dental pulp stem cells, including stem cells from apical papilla (SCAP) and subpopulations of pulp stem cells. Bone marrow mesenchymal stem cells (BMMSCs) and adipose tissue-derived MSCs (ADMSCs) have also been shown to regenerate pulp-like tissue. In contrast, the cell-free approach has not produced convincing evidence on pulp regeneration. However, one crucial concept has not been considered nor defined in the field of pulp/dentin regeneration and that is the critical size defect of dentin and pulp. Without such consideration and definition, it is difficult to predict or anticipate the extent of cell-free pulp regeneration that would occur. By reasoning, cell-free therapy is unlikely to regenerate an organ/tissue after total loss. Similarly, after a total loss of pulp, it is unlikely to regenerate without using exogenously introduced cells. A cell homing approach may provide a limited amount of tissue regeneration. Although stem cell-based pulp/dentin regeneration has shown great promise, clinical trials are difficult to launch at present. This article will address several issues that challenge and hinder the clinical applications of pulp/dentin regeneration which need to be overcome before stem cell-based pulp/dentin regeneration can occur in the clinic.

  1. C-myb Regulates Autophagy for Pulp Vitality in Glucose Oxidative Stress.

    Science.gov (United States)

    Lee, Y H; Kim, H S; Kim, J S; Yu, M K; Cho, S D; Jeon, J G; Yi, H K

    2016-04-01

    Diabetes mellitus is closely related to oral-complicated diseases by oxidative stress. This study investigates whether cellular myeloblastosis (c-myb) could protect human dental pulp cells against glucose oxidative stress and regulate autophagy activity for pulp vitality. Diabetes mellitus was induced by streptozotocin in Sprague-Dawley rats, and their pulp tissue in teeth was analyzed in terms of pulp cavity and molecules by hematoxylin and eosin and immunohistochemistry staining. Human dental pulp cells were serially subcultured and treated with glucose oxidase in the presence of elevated glucose to generate glucose oxidative stress. The replication-deficient adenovirus c-myb and small interfering RNA c-myb were introduced for c-myb expression. The pulp tissue from the diabetic rats was structurally different from normal tissue in terms of narrow pulp capacity, reduced c-myb, and dentinogenesis molecules. Glucose oxidase treatment decreased c-myb and dentinogenesis molecules (bone morphogenetic protein 2 and 7, dentin matrix protein 1, and dentin sialophosphoprotein) in human dental pulp cells. However, overexpression of c-myb by adenovirus c-myb increased dentinogenesis, autophagy molecules (autophagy protein 5, microtubule-associated protein 1A/1B-light chain 3, and Beclin-1), and cell survival via p-AMPK/AKT signaling even with glucose oxidative stress. In contrast, the lack of c-myb decreased the above molecules and cell survival by downregulating p-AMPK/AKT signaling. The results indicate that diabetes leads to irreversible damage to dental pulp, which is related to downexpression of autophagy via the p-AMPK/AKT pathway by decline of c-myb. The findings of this study provide a new insight that c-myb could ameliorate autophagy activity and that it is applicable for monitoring complicated diseases of dental pulp. The involvement of c-myb in pulp pathology could serve a therapeutic target in oral-complicated diseases. © International & American Associations

  2. Absent in melanoma 2 (AIM2) in rat dental pulp mediates the inflammatory response during pulpitis.

    Science.gov (United States)

    Wang, Yafei; Zhai, Shafei; Wang, Haijing; Jia, Qian; Jiang, Wenkai; Zhang, Xiao; Zhang, Ansheng; Liu, Jun; Ni, Longxing

    2013-11-01

    In recent years, the inflammasome has been determined to play an important role in inflammatory diseases. However, the role of the inflammasome in pulpitis remains unclear. Absent in melanoma 2 (AIM2) is a type of inflammasome that recognizes cytosolic double stranded DNA and forms a caspase-1-activating inflammasome with apoptosis-associated speck-like protein containing a caspase activating recruiting domain. In this study, we determined whether AIM2 was expressed in pulp cells and defined the role of AIM2 in the initiation of inflammation within the dental pulp. In the in vivo study, the right maxillary molars from male adult Sprague-Dawley rats (250-350 g) were exposed to the pulp. In the in vitro study, the pulp cells isolated from the mandibular incisors of the Sprague-Dawley rats (2 weeks) were conventionally cultured. Immunofluorescence staining was used to determine the expression and distribution of AIM2 in the rat dental pulp tissues and cells in the presence or absence of inflammatory stimulation. Western blotting and real-time polymerase chain reaction were performed to determine whether there was a correlation between AIM2 expression levels and inflammation both in vivo and in vitro. In healthy dental pulp tissues and cells, AIM2 was only detected in the odontoblast layer. Stimulation significantly increased AIM2 expression in both the dental pulp tissues and cultured cells. The mRNA and protein levels of AIM2 were significantly up-regulated in response to inflammatory stimulation in a dose-dependent manner. Moreover, we also found that AIM2 expression correlated with interleukin-1 levels. These results reveal a direct relationship between the AIM2 inflammasome and pulpitis. Our study demonstrates that AIM2 is expressed in dental pulp tissues and mediates the inflammatory response during pulpitis. Therapeutic interventions aimed at reducing AIM2 expression may be beneficial in the treatment of pulpitis. Copyright © 2013 American Association of

  3. Mesta/Kenaf as raw material for Kraft pulping

    Energy Technology Data Exchange (ETDEWEB)

    Saraf, V. P.; Shiveshwar, R. M.; Meshramkar, P. M.; Deb, U. K.; Jaspal, N. S.; Biyani, B. P.

    1980-03-15

    In recent years, the shortage of conventional raw materials for pulping has intensified a world-wide search for alternate raw materials. Among other cellulosic materials, attention has focused on agricultural residues and annual plants. In this category, Kenaf, also called Mesta, has been studied as a fiber source in many countries including the USA, Phillippines and India. A comprehensive study on kraft pulping of mesta was carried out where the fiber morphology, pulping, bleaching and papermaking characteristics were investigated. Black liquor properties were also determined. The results of this study are reported and compared to the conventional raw material bamboo. The investigation has shown that mesta is easily pulped and bleached and forms sheets of excellent strength properties, comparable to or better than bamboo pulps. The black liquor from mesta is similar to bamboo in calorific value but is very low in silica, which is highly desirable from the chemical recovery point of view. As with all agricultural residues and annual plants, mesta has a high bulk per unit weight. The problems arising from this, e.g., baling, transportation, proper utilization of digester capacity, have to be studied before mesta can be widely accepted in the paper industry.

  4. Rice straw pulp obtained by using various methods.

    Science.gov (United States)

    Rodríguez, Alejandro; Moral, Ana; Serrano, Luis; Labidi, Jalel; Jiménez, Luis

    2008-05-01

    Rice straw was used as an alternative raw material to obtain cellulosics pulps. Pulping was done by using classics reagents as soda (with anthraquinone and parabenzoquinone as aditives), potassium hydroxide and Kraft process. The holocellulose, alpha-cellulose and lignin contents of rice straw (viz. 60.7, 41.2 and 21.9 wt%, respectively) are similar to those of some woody raw materials such as pine and eucalyptus, and various non-wood materials including olive tree prunings, wheat straw and sunflower stalks. Pulping tests were conducted by using soda, soda and anthraquinone at 1 wt%, soda and parabenzoquinone at 1 wt%, potassium hydroxide and sodium sulphate (Kraft process) under two different sets of operating conditions, namely: (a) a 10 wt% reagent concentration, 170 degrees C and 60 min; and (b) 15 wt% reagent, 180 degrees C and 90 min. The solid/liquid ratio was 6 in both cases. Paper sheets made from pulp extracted by cooking with soda (15 wt%) and AQ (1 wt%) at 180 degrees C and 90 min pulp exhibit the best drainage index, breaking length, stretch and burst index (viz. 23 degrees SR, 3494 m, 3.34% and 2.51 kN/g, respectively).

  5. Melatonin attenuates inflammation of acute pulpitis subjected to dental pulp injury

    Science.gov (United States)

    Li, Ji-Guo; Lin, Jia-Ji; Wang, Zhao-Ling; Cai, Wen-Ke; Wang, Pei-Na; Jia, Qian; Zhang, An-Sheng; Wu, Gao-Yi; Zhu, Guo-Xiong; Ni, Long-Xing

    2015-01-01

    Acute pulpitis (AP), one of the most common diseases in the endodontics, usually causes severe pain to the patients, which makes the search for therapeutic target of AP essential in clinic. Toll-like receptor 4 (TLR4) signaling is widely involved in the mechanism of pulp inflammation, while melatonin has been reported to have an inhibition for a various kinds of inflammation. We hereby studied whether melatonin can regulate the expression of TLR4/NF-ĸB signaling in the pulp tissue of AP and in human dental pulp cells (HDPCs). Two left dental pulps of the adult rat were drilled open to establish the AP model, and the serum levels of melatonin and pro-inflammatory cytokines, including interleukin 1β (IL-1β), interleukin 18 (IL-18) and tumor necrosis factor α (TNF-α), were assessed at 1, 3 and 5 d post injury. At the same time points, the expression of TLR4 signaling in the pulp was explored by quantitative real-time PCR and immunohistochemistry. The AP rats were administered an abdominal injection of melatonin to assess whether melatonin rescued AP and TLR4/NF-ĸB signaling. Dental pulp injury led to an approximately five-day period acute pulp inflammation and necrosis in the pulp and a significant up-regulation of IL-1β, IL-18 and TNF-α in the serum. ELISA results showed that the level of melatonin in the serum decreased due to AP, while an abdominal injection of melatonin suppressed the increase in serum cytokines and the percentage of necrosis at the 5 d of the injured pulp. Consistent with the inflammation in AP rats, TLR4, NF-ĸB, TNF-α and IL-1β in the pulp were increased post AP compared with the baseline expression. And melatonin showed an inhibition on TLR4/NF-ĸB signaling as well as IL-1β and TNF-α production in the pulp of AP rats. Furthermore, melatonin could also regulate the expression of TLR4/NF-ĸB signaling in LPS-stimulated HDPCs. These data suggested that dental pulp injury induced AP and reduced the serum level of melatonin and that

  6. Gamma ray induced chromophore modification of softwood thermomechanical pulp

    International Nuclear Information System (INIS)

    Robert, S.; Daneault, C.; Viel, C.; Lepine, F.

    1992-01-01

    This study focuses on bleaching a softwood (black spruce, balsam fur) thermomechanical pulp with gamma rays. Gamma rays are known for their enormous penetrating power, along with their ionizing properties. They can generate highly energetic radicals capable of oxidizing lignin chromophores. The authors studied the influence of isopropyl alcohol, sodium borohydride, oxygen, hydrogen peroxide, nitrogen dioxide and water along with gamma ray irradiation of the pulps. The authors measured the optimal dose and dose rate, along with the influence of the radical scavengers like oxygen on the bleaching effect of gamma irradiated pulps. They observe various degrees of bleaching of these pulps. Evidence relates this bleaching to the generation of perhydroxyl anions upon irradiation of water. Also, they were able to pinpoint the influence of the dose rate on the rate of formation and disappearance of these perhydroxyl anions and their influence on bleaching kinetics. Stability toward photoyellowing, and photoyellowing's kinetic of papers from these pulps was also studied

  7. Holdup time measurement by radioactive tracers in pulp production

    International Nuclear Information System (INIS)

    Roetzer, H.; Donhoffer, D.

    1988-12-01

    A batch of pulp was to be labelled before passing two bleaching towers of a pulp plant. Activated glass fibres were used as a tracer, which contained 24-Na with a half-life of 15 hours. It was shown in laboratory tests, that the glass fibres were suitable for transport studies of wood pulp. For use in the tests the fibres were activated and suspended in water. Due to the small diameter of the fibres (2-5 micrometers) this suspension shows physical properties very similar to the pulp. For detection six scintillation probes were mounted at different positions outside the bleaching tower. Radiation protection during the test was very easy due to the low total activity of the tracer material. Residence time distributions for both towers were measured. The successful tracer experiments show, that the method of labelling is suited for investigations of material transport in the pulp and paper industry. 3 figs., 11 refs., 2 tabs. (Author)

  8. Microarray evaluation of age-related changes in human dental pulp.

    Science.gov (United States)

    Tranasi, Michelangelo; Sberna, Maria Teresa; Zizzari, Vincenzo; D'Apolito, Giuseppe; Mastrangelo, Filiberto; Salini, Luisa; Stuppia, Liborio; Tetè, Stefano

    2009-09-01

    The dental pulp undergoes age-related changes that could be ascribed to physiological, defensive, or pathological irritant-induced changes. These changes are regulated by pulp cell activity and by a variety of extracellular matrix (ECM) macromolecules, playing important roles in growth regulation, tissue differentiation and organization, formation of calcified tissue, and defense mechanisms and reactions to inflammatory stimuli. The aim of this research was to better understand the genetic changes that underlie the histological modification of the dental pulp in aging. The gene expression profile of the human dental pulp in young and older subjects was compared by RNA microarray analysis that allowed to simultaneously analyze the expression levels of thousands of genes. Data were statistically analyzed by Significance Analysis of Microarrays (SAM) Ingenuity Pathway Analysis (IPA) software. Semiquantitative and real-time reverse-transcriptase polymerase chain reaction analyses were performed to confirm the results. Microarray analysis revealed several differentially expressed genes that were categorized in growth factors, transcription regulators, apoptosis regulators, and genes of the ECM. The comparison analysis showed a high expression level of the biological functions of cell and tissue differentiation, development, and proliferation and of the immune, lymphatic, and hematologic system in young dental pulp, whereas the pathway of apoptosis was highly expressed in older dental pulp. Expression profile analyses of human dental pulp represent a sensible and useful tool for the study of mechanisms involved in differentiation, growth and aging of human dental pulp in physiological and pathological conditions.

  9. Synthetic Light-Curable Polymeric Materials Provide a Supportive Niche for Dental Pulp Stem Cells.

    Science.gov (United States)

    Vining, Kyle H; Scherba, Jacob C; Bever, Alaina M; Alexander, Morgan R; Celiz, Adam D; Mooney, David J

    2018-01-01

    Dental disease annually affects billions of patients, and while regenerative dentistry aims to heal dental tissue after injury, existing polymeric restorative materials, or fillings, do not directly participate in the healing process in a bioinstructive manner. There is a need for restorative materials that can support native functions of dental pulp stem cells (DPSCs), which are capable of regenerating dentin. A polymer microarray formed from commercially available monomers to rapidly identify materials that support DPSC adhesion is used. Based on these findings, thiol-ene chemistry is employed to achieve rapid light-curing and minimize residual monomer of the lead materials. Several triacrylate bulk polymers support DPSC adhesion, proliferation, and differentiation in vitro, and exhibit stiffness and tensile strength similar to existing dental materials. Conversely, materials composed of a trimethacrylate monomer or bisphenol A glycidyl methacrylate, which is a monomer standard in dental materials, do not support stem cell adhesion and negatively impact matrix and signaling pathways. Furthermore, thiol-ene polymerized triacrylates are used as permanent filling materials at the dentin-pulp interface in direct contact with irreversibly injured pulp tissue. These novel triacrylate-based biomaterials have potential to enable novel regenerative dental therapies in the clinic by both restoring teeth and providing a supportive niche for DPSCs. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. A comparative study of BioAggregate and ProRoot MTA on adhesion, migration, and attachment of human dental pulp cells.

    Science.gov (United States)

    Zhu, Lingxin; Yang, Jingwen; Zhang, Jie; Peng, Bin

    2014-08-01

    The aim of the present study was to evaluate the effects of a novel bioceramic nanoparticular cement, BioAggregate (Innovative Bioceramix, Vancouver, BC, Canada), on the adhesion, migration, and attachment of human dental pulp cells (HDPCs) and to compare its performance with that of ProRoot mineral trioxide aggregate (MTA) (Dentsply, Tulsa, OK). Primary cultured HDPCs were treated with various dilutions of BioAggregate and MTA extracts to assess the cell viability using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan). Cell adhesion assay was performed using type I collagen-coated plates. An in vitro scratch wound healing model was used to determine cell migration. Focal adhesion formation and cytoskeleton organization were further examined by double immunofluorescence labeling for vinculin and fibrous actin. To assess cell attachment, HDPCs were directly seeded onto the material surfaces and observed by scanning electron microscopy. HDPCs exposed to BioAggregate extracts showed the highest viabilities at all extract concentrations at 24 and 48 hours, whereas cells exposed to original MTA extracts displayed suppressed viabilities at 72 hours compared with the control. Treatment with BioAggregate extracts enhanced cellular adhesion and migration of HDPCs in a concentration-dependent manner, which was superior to the effects induced by MTA extracts. Immunofluorescence staining indicated that both BioAggregate and MTA optimized focal adhesion formation and stress fiber assembly. Furthermore, scanning electron microscopic analysis revealed that HDPCs attached onto BioAggregate were more flattened and exhibited better spreading than cells on MTA. BioAggregate is able to promote cellular adhesion, migration, and attachment of HDPCs, indicating its excellent cytocompatibility. Therefore, BioAggregate appears to be a possible alternative to MTA for pulp capping. Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  11. Dry Kraft Pulping at Ambient Pressure for Cost Effective Energy Saving and Pollution Deduction

    Energy Technology Data Exchange (ETDEWEB)

    Yulin Deng; Art Ragauskas

    2012-08-28

    Sponsored by the DOE Industrial Energy Efficiency Grand Challenge program, our research team at the Georgia Institute of Technology conducted laboratory studies and confirmed the concept of making wood pulp using a dry pulping technology. This technology is a new process different from any prior pulping technology used in Kraft and CTMP pulping. Three different kinds of dry pulping methods were investigated. (a) Dry Pulping at Atmospheric Pressure: The first one is to dry and bake the pretreated woodchips in a conventional oven at atmospheric pressure without the use of a catalyst. (b) Dry Pulping at Reduced Pressure: The second method is to dry the pretreated woodchips first in a vacuum oven in the presence of anthraquinone (AQ) as a pulping catalyst, followed by baking at elevated temperature. (c) Liquid Free Chemical Pulping, LFCP. The third method is to first remove the free water of pretreated woodchips, followed by dry pulping using a conventional Kraft pulping digester with AQ and triton as additives. Method one: Experimental results indicated that Dry Pulping at Atmospheric Pressure could produce pulp with higher brightness and lower bulk than conventional Kraft pulp. However, tensile strength of the acquired pulp is much lower than traditional Kraft pulp, and their Kappa number and energy consumption are higher than conventional Kraft pulp. By fully analyzing the results, we concluded that wood fibers might be damaged during the drying process at elevated temperature. The main reason for wood fiber damage is that a long drying time was used during evaporation of water from the woodchips. This resulted in an un-uniform reaction condition on the woodchips: the outside layer of the woodchips was over reacted while inside the woodchips did not reacted at all. To solve this problem, dry pulping at reduced pressure was investigated. Method two: To achieve uniform reaction throughout the entire reaction system, the water inside the pretreated woodchips was

  12. Integrated chemical plants at the pulp mill

    Energy Technology Data Exchange (ETDEWEB)

    Ehtonen, P.; Hurme, M.; Jaervelaeinen, M.

    1995-12-31

    The goal of this paper is to present how the chemical plants can be integrated to the pulp mill. The integration renders possible to balance the chemical consumptions. The total mass balance of a pulp mill with the incoming fuel material and the outgoing waste and flue gases are discussed. The balance figures are presented for the chemicals of the modern fibre line, which will produce fully bleached softwood pulp with an improved effluent quality. The main benefits are lower chemical and transportation costs. The principal over-all plant process block diagrams and process descriptions are presented. The presented info system provides real time information on process and production status at overall mill and department levels. (author)

  13. Cynara cardunculus L. alkaline pulps: alternatives fibres for paper and paperboard production.

    Science.gov (United States)

    Abrantes, S; Amaral, M E; Costa, A P; Duarte, A P

    2007-11-01

    The pulping of Cynara cardunculus L. (cardoon) was performed under conditions for kraft, kraft-AQ and soda-AQ processes. The best results in terms of delignification degree, expressed as kappa number, pulp viscosity and screened yield, were obtained for the kraft-AQ process with 0.20% of anthraquinone (AQ). The papermaking potential of the selected pulp was studied attending to biometric fibre characterisation, refining aptitude, optical and strength properties. All properties were compared against a Eucalyptus globulus pulp at different refining degrees. The cardoon pulp was also evaluated concerning its potential to board manufacture, alone and in mixtures with pine pulp, giving rise to promising results for liner manufacture.

  14. Controlling the shapes of silver nanocrystals with different capping agents.

    Science.gov (United States)

    Zeng, Jie; Zheng, Yiqun; Rycenga, Matthew; Tao, Jing; Li, Zhi-Yuan; Zhang, Qiang; Zhu, Yimei; Xia, Younan

    2010-06-30

    This paper provides direct evidence to support the role of a capping agent in controlling the evolution of Ag seeds into nanocrystals with different shapes. Starting with single-crystal seeds (spherical or cubic in shape), we could selectively obtain Ag octahedrons enclosed by {111} facets and nanocubes/nanobars enclosed by {100} facets by adding sodium citrate (Na(3)CA) and poly(vinyl pyrrolidone) (PVP), respectively, as a capping agent while all other parameters were kept the same. This research not only offers new insights into the role played by a capping agent in shape-controlled synthesis but also provides, for the first time, Ag octahedrons as small as 40 nm in edge length for optical and spectroscopic studies.

  15. The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity

    Directory of Open Access Journals (Sweden)

    Minako Ogino

    2016-05-01

    Full Text Available The large (L protein of rabies virus (RABV plays multiple enzymatic roles in viral RNA synthesis and processing. However, none of its putative enzymatic activities have been directly demonstrated in vitro. In this study, we expressed and purified a recombinant form of the RABV L protein and verified its guanosine 5′-triphosphatase and GDP polyribonucleotidyltransferase (PRNTase activities, which are essential for viral mRNA cap formation by the unconventional mechanism. The RABV L protein capped 5′-triphosphorylated but not 5′-diphosphorylated RABV mRNA-start sequences, 5′-AACA(C/U, with GDP to generate the 5′-terminal cap structure G(5′ppp(5′A. The 5′-AAC sequence in the substrate RNAs was found to be strictly essential for RNA capping with the RABV L protein. Furthermore, site-directed mutagenesis showed that some conserved amino acid residues (G1112, T1170, W1201, H1241, R1242, F1285, and Q1286 in the PRNTase motifs A to E of the RABV L protein are required for cap formation. These findings suggest that the putative PRNTase domain in the RABV L protein catalyzes the rhabdovirus-specific capping reaction involving covalent catalysis of the pRNA transfer to GDP, thus offering this domain as a target for developing anti-viral agents.

  16. Improvement of Pulping Uniformity by Measurement of Single Fiber Kappa Number

    Energy Technology Data Exchange (ETDEWEB)

    Richard R. Gustafson; James B. Callis

    2001-11-20

    A method to measure the kappa of single fibers by staining with a fluorescent dye, Acridine Orange (AO), has been developed. This method is now applied to develop and automated flow-through instrument that permits routine kappa analysis on thousands of images of AO stained fibers to give the fiber kappa number distribution of a pulp sample in a few minutes. The design and operation of the instrument are similar to that of a flow cytometer but with the addition of extensive fiber imaging capability. Fluorescence measurements in the flow-through instrument are found to be consistent with those made with fluorescence microscope provided the signal processing in the flow-thou instrument is handled propertly. The kappa distributions of pulps that were analyzed by means of a density gradient column are compared to those measured with the flow-through instrument with good results. The kappa distributions of various laboratory pulps and commercial pulps have been measured. It has been found that all pulps are non-uniform but that ommercial pulps generally have broader kappa distributions thatn their laboratory counterparts. The effects of different pulping methods and chip pretreatments on pulp uniformity are discussed in the report. Finally, the application of flow-through fluorescence technology to other single fiber measurements are presented.

  17. Effects of mineral trioxide aggregate and formocresol on vital pulp after pulpotomy of primary molars: An in vivo study

    Directory of Open Access Journals (Sweden)

    Brinda Godhi

    2011-01-01

    Full Text Available Background: Preservation of pulp vitality is of utmost importance to the normal physiological functioning of tooth in situ and physiological process of exfoliation in a tooth affected by disease or trauma. Pulpotomy serves such a purpose using various medicaments applied directly on vital pulp. Aim: The aim of this study was to evaluate clinically and radiographically the effects of mineral trioxide aggregate (MTA and formocresol (FC as a pulp dressing after coronal pulp amputation (pulpotomy in primary molars prospectively over a period of 1 year. Materials and Methods: Thirty-three healthy children, aged between 5 and 8 years, requiring pulp therapy were selected after clinical and radiographic assessment. A total of 50 maxillary and mandibular primary molars were treated by the conventional pulpotomy technique. The teeth were divided into two equal groups. In Group A, FC was used as the pulp dressing agent, and in Group B, MTA was used before restoration with stainless steel crowns. The research employed was a prospective study. The teeth treated were assessed postoperatively after 1, 3, 6 and 12 months. The observations were tabulated and statistically analyzed. Results: Clinically, both the groups showed 100% success at 1, 3, 6 and 12 months. At 3 months, the radiographic success rates of FC and MTA were 92% and 96%, respectively, and at 6 and 12 months, the radiographic success rates of FC and MTA were 88% and 96%, respectively. Conclusion: MTA showed a higher success rate than FC and may be a favorable material for pulpotomy in primary molars whose pulps have been compromised by a carious or mechanical pulp exposure

  18. Dental Pulp Defence and Repair Mechanisms in Dental Caries

    OpenAIRE

    Farges, Jean-Christophe; Alliot-Licht, Brigitte; Renard, Emmanuelle; Ducret, Maxime; Gaudin, Alexis; Smith, Anthony J.; Cooper, Paul R.

    2015-01-01

    Dental caries is a chronic infectious disease resulting from the penetration of oral bacteria into the enamel and dentin. Microorganisms subsequently trigger inflammatory responses in the dental pulp. These events can lead to pulp healing if the infection is not too severe following the removal of diseased enamel and dentin tissues and clinical restoration of the tooth. However, chronic inflammation often persists in the pulp despite treatment, inducing permanent loss of normal tissue and red...

  19. Properties of cellulose derivatives produced from radiation-Modified cellulose pulps

    International Nuclear Information System (INIS)

    Iller, Edward; Stupinska, Halina; Starostka, Pawel

    2007-01-01

    The aim of project was elaboration of radiation methods for properties modification of cellulose pulps using for derivatives production. The selected cellulose pulps were exposed to an electron beam with energy 10 MeV in a linear accelerator. After irradiation pulps underwent the structural and physico-chemical investigations. The laboratory test for manufacturing carboxymethylocellulose (CMC), cellulose carbamate (CC) and cellulose acetate (CA) with cellulose pulps irradiated dose 10 and 15 kGy have been performed. Irradiation of the pulp influenced its depolimerisation degree and resulted in the drop of viscosity of CMC. However, the expected level of cellulose activation expressed as a rise of the substitution degree or increase of the active substance content in the CMC sodium salt was not observed. In the case of cellulose esters (CC, CA) formation, the action of ionising radiation on cellulose pulps with the dose 10 and 15 kGy enables obtaiment of the average values of polimerisation degree as required for CC soluble in aqueous sodium hydroxide solution. The properties of derivatives prepared by means of radiation and classic methods were compared

  20. A nanobody targeting the F-actin capping protein CapG restrains breast cancer metastasis.

    Science.gov (United States)

    Van Impe, Katrien; Bethuyne, Jonas; Cool, Steven; Impens, Francis; Ruano-Gallego, David; De Wever, Olivier; Vanloo, Berlinda; Van Troys, Marleen; Lambein, Kathleen; Boucherie, Ciska; Martens, Evelien; Zwaenepoel, Olivier; Hassanzadeh-Ghassabeh, Gholamreza; Vandekerckhove, Joël; Gevaert, Kris; Fernández, Luis Ángel; Sanders, Niek N; Gettemans, Jan

    2013-12-13

    Aberrant turnover of the actin cytoskeleton is intimately associated with cancer cell migration and invasion. Frequently however, evidence is circumstantial, and a reliable assessment of the therapeutic significance of a gene product is offset by lack of inhibitors that target biologic properties of a protein, as most conventional drugs do, instead of the corresponding gene. Proteomic studies have demonstrated overexpression of CapG, a constituent of the actin cytoskeleton, in breast cancer. Indirect evidence suggests that CapG is involved in tumor cell dissemination and metastasis. In this study, we used llama-derived CapG single-domain antibodies or nanobodies in a breast cancer metastasis model to address whether inhibition of CapG activity holds therapeutic merit. We raised single-domain antibodies (nanobodies) against human CapG and used these as intrabodies (immunomodulation) after lentiviral transduction of breast cancer cells. Functional characterization of nanobodies was performed to identify which biochemical properties of CapG are perturbed. Orthotopic and tail vein in vivo models of metastasis in nude mice were used to assess cancer cell spreading. With G-actin and F-actin binding assays, we identified a CapG nanobody that binds with nanomolar affinity to the first CapG domain. Consequently, CapG interaction with actin monomers or actin filaments is blocked. Intracellular delocalization experiments demonstrated that the nanobody interacts with CapG in the cytoplasmic environment. Expression of the nanobody in breast cancer cells restrained cell migration and Matrigel invasion. Notably, the nanobody prevented formation of lung metastatic lesions in orthotopic xenograft and tail-vein models of metastasis in immunodeficient mice. We showed that CapG nanobodies can be delivered into cancer cells by using bacteria harboring a type III protein secretion system (T3SS). CapG inhibition strongly reduces breast cancer metastasis. A nanobody-based approach offers

  1. Scaffolds to Control Inflammation and Facilitate Dental Pulp Regeneration

    Science.gov (United States)

    Colombo, John S.; Moore, Amanda N.; Hartgerink, Jeffrey D.; D’Souza, Rena N.

    2014-01-01

    In dentistry, the maintenance of a vital dental pulp is of paramount importance, as teeth devitalized by root canal treatment may become more brittle and prone to structural failure over time. Advanced carious lesions can irreversibly damage the dental pulp by propagating a sustained inflammatory response throughout the tissue. While the inflammatory response initially drives tissue repair, sustained inflammation has an enormously destructive effect on the vital pulp, eventually leading to total necrosis of the tissue and necessitating its removal. The implications of tooth devitalization have driven significant interest in the development of bioactive materials that facilitate the regeneration of damaged pulp tissues by harnessing the capacity of the dental pulp for self-repair. In considering the process by which pulpitis drives tissue destruction, it is clear that an important step in supporting the regeneration of pulpal tissues is the attenuation of inflammation. Macrophages, key mediators of the immune response, may play a critical role in the resolution of pulpitis due to their ability to switch to a pro-resolution phenotype. This process can be driven by the resolvins, a family of molecules derived from fatty acids that show great promise as therapeutic agents. In this review, we outline the importance of preserving the capacity of the dental pulp to self-repair through the rapid attenuation of inflammation. Potential treatment modalities, such as shifting macrophages to a pro-resolving phenotype with resolvins are described, and a range of materials known to support the regeneration of dental pulp are presented. PMID:24698696

  2. Idiopathic dental pulp calcifications in a tertiary care setting in South India.

    Science.gov (United States)

    Satheeshkumar, P S; Mohan, Minu P; Saji, Sweta; Sadanandan, Sudheesh; George, Giju

    2013-01-01

    Dental pulp calcifications are unique and represent the dental pulp regenerative process. Dental pulp calcifications are sometimes routine findings in oral radiographs and may later serve as an important diagnostic criterion for a hidden aspect of systemic illness. The purpose of this study was to assess the patterns and prevalence of idiopathic dental pulp calcifications in a tertiary care setting in South India. A total of 227 patients were included in the study fulfilling the inclusion criteria. Age range of the study population was from 15 to 70 years. Teeth were examined under digital panoramic radiograph. The presence or absence of pulp stones was recorded. The presence of pulp stone were categorized according to the types classified as Type I, Type IA, Type II, Type IIA, Type II B, and Type III. The frequency of occurrence of pulp stones with sex, tooth type, dental arches, and types were compared with the types of calcification. Total no. of patients with pulpal calcification were 227 [females 133 (58.59%) and males 94 (41.40%)]. The most common type between both sexes was Type I (48%). Total no. of teeth with calcification was 697; maxilla (48%), mandible (52%). The prevalence of pulp stone was found to be higher in the molars in both the arches. Most no. of pulp stones are reported at the third and fourth decade of life. Idiopathic dental pulp calcifications are incidental radiographic findings of the pulp tissue and also may be an indicator of underlying disease.

  3. Partial pulp necrosis caused by excessive orthodontic force

    Directory of Open Access Journals (Sweden)

    Min-Young Kim

    2011-03-01

    Full Text Available As the dental pulp is encased with a rigid, noncompliant shell, changes in pulpal blood flow or vascular tissue pressure can have serious implication for the health of pulp. Numerous studies have demonstrated that orthodontic force application may influence both blood flow and cellular metabolism, leading degenerative and/or inflammatory responses in the dental pulp. The aim of this case report is to present a case about tooth with chronic periapical abscess which showed normal vital responses. Excessive orthodontic force is thought to be the prime cause of partial pulp necrosis. Owing to remaining vital tissue, wrong dianosis can be made, and tooth falsely diagnosed as vital may be left untreated, causing the necrotic tissue to destroy the supporting tissuses. Clinician should be able to utilize various diagnostic tools for the precise diagnosis, and be aware of the endodontic-orthodontic inter-relationship.

  4. Durability of pulp fiber-cement composites

    Science.gov (United States)

    Mohr, Benjamin J.

    Wood pulp fibers are a unique reinforcing material as they are non-hazardous, renewable, and readily available at relatively low cost compared to other commercially available fibers. Today, pulp fiber-cement composites can be found in products such as extruded non-pressure pipes and non-structural building materials, mainly thin-sheet products. Although natural fibers have been used historically to reinforce various building materials, little scientific effort has been devoted to the examination of natural fibers to reinforce engineering materials until recently. The need for this type of fundamental research has been emphasized by widespread awareness of moisture-related failures of some engineered materials; these failures have led to the filing of national- and state-level class action lawsuits against several manufacturers. Thus, if pulp fiber-cement composites are to be used for exterior structural applications, the effects of cyclical wet/dry (rain/heat) exposure on performance must be known. Pulp fiber-cement composites have been tested in flexure to examine the progression of strength and toughness degradation. Based on scanning electron microscopy (SEM), environmental scanning electron microscopy (ESEM), energy dispersive spectroscopy (EDS), a three-part model describing the mechanisms of progressive degradation has been proposed: (1) initial fiber-cement/fiber interlayer debonding, (2) reprecipitation of crystalline and amorphous ettringite within the void space at the former fiber-cement interface, and (3) fiber embrittlement due to reprecipitation of calcium hydroxide filling the spaces within the fiber cell wall structure. Finally, as a means to mitigate kraft pulp fiber-cement composite degradation, the effects of partial portland cement replacement with various supplementary cementitious materials (SCMs) has been investigated for their effect on mitigating kraft pulp fiber-cement composite mechanical property degradation (i.e., strength and toughness

  5. Central Air-Conditioning Plant (CAP) extension

    International Nuclear Information System (INIS)

    Shetty, P.S.; Kaul, S.K.; Mishra, H.

    2017-01-01

    Central Air-Conditioning Plant (CAP) and its associated chilled water network of BARC is one among the largest central plants in India for such application. The plant was planned in 1960s to cater to the air-conditioning and process water requirements of laboratories, workshops and buildings spread over a distance of 1.5 Km in three directions from CAP through underground network of chilled water pipelines. The plant was designed for a total capacity of 6600 TR. The present installed capacity of the plant is 7250 TR. The connected load at present is 9800 TR. After the XII plan capacity will be augmented to 7650 TR. The connected load is expected to cross 11,000 TR after the commissioning of new Engg. Halls 9, 10 and 11

  6. Comparative study of percentage yield of pulp from various Nigerian ...

    African Journals Online (AJOL)

    user

    also other applications like in the chemical industry. (Biermann, 1993). Therefore pulp is a very .... digester was maintained at an operating condition of. 170°C, pressure of 2 bars, with liquor to wood ratio of 4:1. Cooking ... The effects of different concentrations of pulping liquor on the yield of pulp from the wood species are ...

  7. Alkaline pulping with additives of date palm rachis and leaves from Sudan.

    Science.gov (United States)

    Khristova, P; Kordsachia, O; Khider, T

    2005-01-01

    Soda-anthraquinone (soda-AQ), alkaline sulphite-anthraquinone (AS-AQ) and alkaline sulphite-anthraquinone-methanol (ASAM) pulping of date palm rachis and leaves from Sudan was carried under different conditions, and pulps with variable yields and mechanical properties were obtained. The date palm rachis gave best yields and mechanical properties with the AS-AQ or the ASAM process, while the leaves were best pulped with the soda method with low yield, but very good strength properties. Blending with 10% and 30% kenaf bark pulp was beneficial, especially for the AS-AQ pulps. Totally chlorine free (TCF) bleached rachis pulps were obtained of high brightness and strength properties suitable for use in writing and printing papers.

  8. Method of increasing efficiency of uranium sorption from acid pulp

    International Nuclear Information System (INIS)

    Parobek, P.; Hinterholzinger, O.; Baloun, S.; Homolka, V.; Vanek, J.; Vebr, Z.

    1989-01-01

    Acid pulp containing uranium is adjusted to pH 2.5 to 4 with alkaline agents, such as alkaline pulp, lime milk, finely ground limestone or soda, or a combination thereof. The treated pulp is put into contact with an ion exchanger whose pH has been adjuste to a range of 2.5 to 4. Partial pulp neutralization causes the hydrolysis of the iron present and an overall reduction in salt contents and a significant increase in the ion exchanger sorptio capacity and thus the overall sorption efficiency. The quality o the eluate and of the uranium concentrate improves. (B.S.)

  9. Microarray expression profiling of human dental pulp from single subject.

    Science.gov (United States)

    Tete, Stefano; Mastrangelo, Filiberto; Scioletti, Anna Paola; Tranasi, Michelangelo; Raicu, Florina; Paolantonio, Michele; Stuppia, Liborio; Vinci, Raffaele; Gherlone, Enrico; Ciampoli, Cristian; Sberna, Maria Teresa; Conti, Pio

    2008-01-01

    Microarray is a recently developed simultaneous analysis of expression patterns of thousand of genes. The aim of this research was to evaluate the expression profile of human healthy dental pulp in order to find the presence of genes activated and encoding for proteins involved in the physiological process of human dental pulp. We report data obtained by analyzing expression profiles of human tooth pulp from single subjects, using an approach based on the amplification of the total RNA. Experiments were performed on a high-density array able to analyse about 21,000 oligonucleotide sequences of about 70 bases in duplicate, using an approach based on the amplification of the total RNA from the pulp of a single tooth. Obtained data were analyzed using the S.A.M. system (Significance Analysis of Microarray) and genes were merged according to their molecular functions and biological process by the Onto-Express software. The microarray analysis revealed 362 genes with specific pulp expression. Genes showing significant high expression were classified in genes involved in tooth development, protoncogenes, genes of collagen, DNAse, Metallopeptidases and Growth factors. We report a microarray analysis, carried out by extraction of total RNA from specimens of healthy human dental pulp tissue. This approach represents a powerful tool in the study of human normal and pathological pulp, allowing minimization of the genetic variability due to the pooling of samples from different individuals.

  10. Anti-inflammatory, and antinociceptive effects of Campomanesia adamantium microencapsulated pulp

    Directory of Open Access Journals (Sweden)

    Danieli Z. Viscardi

    Full Text Available ABSTRACT Guavira fruits have antimicrobial, antioxidant, antinociceptive, and anti-inflammatory activities. Spray drying has been widely used in the food industry presenting good retention in bioactive compounds used to transform the pulp/fruit juice into powder form. Therefore, the present study has evaluated the anti-inflammatory and antinociceptive activities of the microencapsulated pulp of Campomanesia adamantium (Cambess. O.Berg, Myrtaceae, by spray drying. Different groups of mice were treated with the doses of 100 and 300 mg/kg of microencapsulated "guavira" pulp and inflammatory parameters were assessed in a carrageenan paw edema-model and leukocyte migration with pleurisy model, while the antinociceptive activity was assessed using the formalin method and CFA-induced hyperalgesia model. A significant reduction in leukocyte migration and in paw edema was observed in rodents in all time after carrageenan injection for both doses of microencapsulated pulp of C. adamantium when compared with control group. Microencapsulated pulp of C. adamantium also reduced licking time at the first (nociceptive and second (inflammatory phases in the formalin model. In CFA-induced cold and mechanical hyperalgesia, depressive behavior, and knee edema, all parameters analyzed were significantly inhibited by microencapsulated pulp of C. adamantium. Microencapsulation by spray drying proved to be a technique that promotes bioavailability and the preservation of bioactive components in guavira pulp.

  11. Cassava Pulp as a Biofuel Feedstock of an Enzymatic Hydrolysis Proces

    Directory of Open Access Journals (Sweden)

    Djuma’ali Djuma’ali

    2013-03-01

    Full Text Available Cassava pulp, a low cost solid byproduct of cassava starch industry, has been proposed as a high potential ethanolic fermentation substrate due to its high residual starch level, low ash content and small particle size of the lignocellulosic fibers. As the economic feasibility depends on complete degradation of the polysaccharides to fermentable glucose, the comparative hydrolytic potential of cassava pulp by six commercial enzymes were studied. Raw cassava pulp (12% w/v, particle size <320 μm hydrolyzed by both commercial pectinolytic (1 and amylolytic (2 enzymes cocktail, yielded 70.06% DE. Hydrothermal treatment of cassava pulp enhanced its susceptibility to enzymatic cleavageas compared to non-hydrothermal treatment raw cassava pulp. Hydrothermal pretreatment has shown that a glucoamylase (3 was the most effective enzyme for hydrolysis process of cassava pulp at temperature 65 °C or 95 °C for 10 min and yielded approximately 86.22% and 90.18% DE, respectively. Enzymatic pretreatment increased cassava pulp vulnerability to cellulase attacks. The optimum conditions for enzymatic pretreatment of 30% (w/v cassava pulp by a potent cellulolytic/ hemicellulolytic enzyme (4 was achieves at 50 °C for 3, meanwhile for liquefaction and saccharification by a thermo-stable α-amylase (5 was achieved at 95 °C for 1 and a glucoamylase (3 at 50 °C for 24 hours, respectively, yielded a reducing sugar level up to 94,1% DE. The high yield of glucose indicates the potential use of enzymatic-hydrothermally treated cassava pulp as a cheap substrate for ethanol production.

  12. /sup 58,60,62/Ni (. cap alpha. ,p) three--nucleon transfer reactions and. cap alpha. optical potential ambiguities

    Energy Technology Data Exchange (ETDEWEB)

    Yuanda, Wang; Xiuming, Bao; Zhiqiang, Mao; Rongfang, Yuan; Keling, Wen; Binyin, Huang; Zhifu, Wang; Shuming, Li; Jianan, Wang; Zuxun, Sun; others, and

    1985-11-01

    The differential cross sections are measured using 26.0 MeV ..cap alpha.. particle for /sup 58,62/Ni(..cap alpha.., ..cap alpha..) /sup 58,62/Ni and /sup 58,62/Ni(..cap alpha..,p) /sup 61,65/Cu reactions as well as 25.4 MeV ..cap alpha.. particle for /sup 60/Ni(..cap alpha.., ..cap alpha..)/sup 69/Ni and /sup 60/Ni(..cap alpha.., p)/sup 63/Cu reactions. Consistent calculations with optical model and ZR DWBA are made for (..cap alpha.., ..cap alpha..) and (..cap alpha.., p) reactions by using of single, two, three and four nucleon optical potential parameters. For elastic scattering due to the ..cap alpha.. optical potential ambiguities, all the above optical potential can reproduce the experimental angular distributions. However, the single, two and three nucleon potential, including the Baird's mass systematics and the Chang's energy systematics of ..cap alpha.. potentials, obviously can not provide a reasonable fitting with the (..cap alpha..,p) reaction experimental data. Only the results from the four nucleon potential is in good agreement with the (..cap alpha..,p) reaction experimental data. This reveals that in the ..cap alpha..-particle induced transfer reactions, the real depth of the ..cap alpha..-nucleus optical potential should be rather deep.

  13. How does the antagonism between capping and anti-capping proteins affect actin network dynamics?

    International Nuclear Information System (INIS)

    Hu Longhua; Papoian, Garegin A

    2011-01-01

    Actin-based cell motility is essential to many biological processes. We built a simplified, three-dimensional computational model and subsequently performed stochastic simulations to study the growth dynamics of lamellipodia-like branched networks. In this work, we shed light on the antagonism between capping and anti-capping proteins in regulating actin dynamics in the filamentous network. We discuss detailed mechanisms by which capping and anti-capping proteins affect the protrusion speed of the actin network and the rate of nucleation of filaments. We computed a phase diagram showing the regimes of motility enhancement and inhibition by these proteins. Our work shows that the effects of capping and anti-capping proteins are mainly transmitted by modulation of the filamentous network density and local availability of monomeric actin. We discovered that the combination of the capping/anti-capping regulatory network with nucleation-promoting proteins introduces robustness and redundancy in cell motility machinery, allowing the cell to easily achieve maximal protrusion speeds under a broader set of conditions. Finally, we discuss distributions of filament lengths under various conditions and speculate on their potential implication for the emergence of filopodia from the lamellipodial network.

  14. EFFECTS OF ALKALINE PRE-IMPREGNATION AND PULPING ON MALAYSIA CULTIVATED KENAF (HIBISCUS CANNABINUS

    Directory of Open Access Journals (Sweden)

    Lin Suan Ang

    2010-05-01

    Full Text Available This study was carried out to identify an appropriate alkaline pulping condition for Malaysia cultivated kenaf (Hibiscus cannabinus L.. The chemical composition of the kenaf bast and core fibers, and also whole stalk with different growing time were examined prior to pulping attempts. The results of various soda-AQ pulping showed that the degree of carbohydrate degradation and delignification increased with the increase of active alkali and cooking temperature, but decreased with the increase of liquor to material (L:M ratio. The most satisfactory properties of pulp and handsheets from bast could be attained by employing soda-AQ pulping with 19.4% active alkali, 0.10% AQ, and L:M ratio of 7:1 cooked for 2 hours at 160˚C. Besides, it was also found that a mild alkaline pre-impregnation prior pulping improved the pulp viscosity and handsheets’ strength properties, especially the tensile index and folding endurance effectively. Moreover, among the three alkaline pulping processes—kraft, kraft-AQ, and soda-AQ—the results of pulp and handsheet properties showed that the soda-AQ pulp was comparable or even slightly of higher quality than the kraft pulps. Between the unbeaten bast and core soda-AQ handsheets, the strength properties of the core were higher than the bast, as the thin-walled core fibers exhibited much better conformability than the thick-walled bast fibers.

  15. Assessment of oxygen saturation in dental pulp of permanent teeth with periodontal disease.

    Science.gov (United States)

    Giovanella, Larissa Bergesch; Barletta, Fernando Branco; Felippe, Wilson Tadeu; Bruno, Kely Firmino; de Alencar, Ana Helena Gonçalves; Estrela, Carlos

    2014-12-01

    In individuals with periodontal disease, dental pulp status should be determined before a treatment plan is made. Pulse oximeters are promising diagnostic tools to evaluate pulp vascularization. This study used pulse oximetry to determine the level of oxygen saturation in dental pulp of intact permanent teeth with periodontal attachment loss (PAL) and gingival recession (GR) and to evaluate the correlation between periodontal disease and level of oxygen saturation in the pulp. This study included 67 anterior teeth of 35 patients; all teeth showed intact crowns, PAL, a periodontal pocket (PP), and GR. The teeth underwent periodontal examination, cold and electric pulp testing, and pulse oximetry measurements. The Pearson correlation coefficient and a linear regression coefficient were calculated to evaluate the degree of correlation between periodontal disease markers (PAL, PP, and GR) and the level of oxygen saturation in dental pulp. These tests also evaluated possible associations between oxygen saturation and cold and electric pulp testing. PAL, PP, and GR had negative correlations with oxygen saturation in dental pulp. Conversely, no statistically significant association was found between oxygen saturation in dental pulp and the response to electric sensibility testing. Oxygen saturation was lower in the pulp of permanent teeth with PAL, PP, and GR, indicating that periodontal disease correlates with the level of oxygen saturation in the pulp. Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  16. Vital pulp therapy in symptomatic immature permanent molars: Report of 3 cases

    Directory of Open Access Journals (Sweden)

    SheikhRezaie MS.

    2009-11-01

    Full Text Available "nEndodontic treatment of immature permanent teeth accompanies with several issues. The primary goal when treating such teeth is to maintain pulp vitality so that root development can occur normally. Indications and requirements for vital pulp therapy include asymptomatic and reversible pulpitis. Also there are controversial opinions regarding the ultimate clinical treatment of the vital pulp therapy techniques. In this manuscript we report 3 cases of immature symptomatic permanent molars with irreversible pulpitis caused by caries exposure of the pulp that have been undergone vital pulp therapy successfully.

  17. Potential dental pulp revascularization and odonto-/osteogenic capacity of a novel transplant combined with dental pulp stem cells and platelet-rich fibrin.

    Science.gov (United States)

    Chen, Yong-Jin; Zhao, Yin-Hua; Zhao, Ya-Juan; Liu, Nan-Xia; Lv, Xin; Li, Qiang; Chen, Fa-Ming; Zhang, Min

    2015-08-01

    Our aim is to investigate the cytobiological effects of autologous platelet-rich fibrin (PRF) on dental pulp stem cells (DPSCs) and to explore the ectopic and orthotopic possibilities of dental pulp revascularization and pulp-dentin complex regeneration along the root canal cavities of the tooth by using a novel tissue-engineered transplant composed of cell-sheet fragments of DPSCs and PRF granules. Canine DPSCs were isolated and characterized by assaying their colony-forming ability and by determining their cell surface markers and osteogenic/adipogenic differentiation potential. The biological effects of autologous PRF on DPSCs, including cell proliferation, alkaline phosphatase (ALP) activity and odonto-/osteogenic gene expression, were then investigated and quantified. A novel transplant consisting of cell-sheet fragments of DPSCs and PRF granules was adopted to regenerate pulp-dentin-like tissues in the root canal, both subcutaneously in nude mice and in the roots of canines. PRF promoted the proliferation of DPSCs in a dose- and time-dependent manner and induced the differentiation of DPSCs to odonto-/osteoblastic fates by increasing the expression of the Alp, Dspp, Dmp1 and Bsp genes. Transplantation of the DPSC/PRF construct led both to a favorable regeneration of homogeneous and compact pulp-like tissues with abundantly distributed blood capillaries and to the deposition of regenerated dentin along the intracanal walls at 8 weeks post-operation. Thus, the application of DPSC/PRF tissue constructs might serve as a potential therapy in regenerative endodontics for pulp revitalization or revascularization.

  18. 47 CFR 54.623 - Cap.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 3 2010-10-01 2010-10-01 false Cap. 54.623 Section 54.623 Telecommunication... Universal Service Support for Health Care Providers § 54.623 Cap. (a) Amount of the annual cap. The annual cap on federal universal service support for health care providers shall be $400 million per funding...

  19. 47 CFR 54.507 - Cap.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 3 2010-10-01 2010-10-01 false Cap. 54.507 Section 54.507 Telecommunication... Universal Service Support for Schools and Libraries § 54.507 Cap. (a) Amount of the annual cap. The annual funding cap on federal universal service support for schools and libraries shall be $2.25 billion per...

  20. Biological evaluation of nanosilver incorporated cellulose pulp for hygiene products

    Energy Technology Data Exchange (ETDEWEB)

    Kavitha Sankar, P.C.; Ramakrishnan, Reshmi; Rosemary, M.J., E-mail: rosemarymj@lifecarehll.com

    2016-04-01

    Cellulose pulp has a visible market share in personal hygiene products such as sanitary napkins and baby diapers. However it offers good surface for growth of microorganisms. Huge amount of research is going on in developing hygiene products that do not initiate microbial growth. The objective of the present work is to produce antibacterial cellulose pulp by depositing silver nanopowder on the cellulose fiber. The silver nanoparticles used were of less than 100 nm in size and were characterised using transmission electron microscopy and X-ray powder diffraction studies. Antibacterial activity of the functionalized cellulose pulp was proved by JIS L 1902 method. The in-vitro cytotoxicity, in-vivo vaginal irritation and intracutaneous reactivity studies were done with silver nanopowder incorporated cellulose pulp for introducing a new value added product to the market. Cytotoxicity evaluation suggested that the silver nanoparticle incorporated cellulose pulp is non-cytotoxic. No irritation and skin sensitization were identified in animals tested with specific extracts prepared from the test material in the in-vivo experiments. The results indicated that the silver nanopowder incorporated cellulose pulp meets the requirements of the standard practices recommended for evaluating the biological reactivity and has good biocompatibility, hence can be classified as a safe hygiene product. - Highlights: • Different amounts of silver nanoparticles (0.2 g–0.4 g/napkin) were added to cellulose pulp. • The silver nanoparticle incorporated cellulose pulp was proved to be antibacterial by JIS L 1902 method. • The minimum concentration of silver required for antibacterial activity with no cytotoxicity has been found out. • In-vivo vaginal irritation and intracutaneous reactivity studies confirmed the biocompatibility of the material.

  1. Biological evaluation of nanosilver incorporated cellulose pulp for hygiene products

    International Nuclear Information System (INIS)

    Kavitha Sankar, P.C.; Ramakrishnan, Reshmi; Rosemary, M.J.

    2016-01-01

    Cellulose pulp has a visible market share in personal hygiene products such as sanitary napkins and baby diapers. However it offers good surface for growth of microorganisms. Huge amount of research is going on in developing hygiene products that do not initiate microbial growth. The objective of the present work is to produce antibacterial cellulose pulp by depositing silver nanopowder on the cellulose fiber. The silver nanoparticles used were of less than 100 nm in size and were characterised using transmission electron microscopy and X-ray powder diffraction studies. Antibacterial activity of the functionalized cellulose pulp was proved by JIS L 1902 method. The in-vitro cytotoxicity, in-vivo vaginal irritation and intracutaneous reactivity studies were done with silver nanopowder incorporated cellulose pulp for introducing a new value added product to the market. Cytotoxicity evaluation suggested that the silver nanoparticle incorporated cellulose pulp is non-cytotoxic. No irritation and skin sensitization were identified in animals tested with specific extracts prepared from the test material in the in-vivo experiments. The results indicated that the silver nanopowder incorporated cellulose pulp meets the requirements of the standard practices recommended for evaluating the biological reactivity and has good biocompatibility, hence can be classified as a safe hygiene product. - Highlights: • Different amounts of silver nanoparticles (0.2 g–0.4 g/napkin) were added to cellulose pulp. • The silver nanoparticle incorporated cellulose pulp was proved to be antibacterial by JIS L 1902 method. • The minimum concentration of silver required for antibacterial activity with no cytotoxicity has been found out. • In-vivo vaginal irritation and intracutaneous reactivity studies confirmed the biocompatibility of the material.

  2. Controlling the Shapes of Silver Nanocrystals with Different Capping Agents

    Energy Technology Data Exchange (ETDEWEB)

    Zeng, J.; Tao, J.; Zheng, Y.; Rycenga, M.; Li, Z.-Y.; Zhang, Q.A.; Zhu, Y.; Xia, Y.

    2010-06-30

    This paper provides direct evidence to support the role of a capping agent in controlling the evolution of Ag seeds into nanocrystals with different shapes. Starting with single-crystal seeds (spherical or cubic in shape), we could selectively obtain Ag octahedrons enclosed by {l_brace}111{r_brace} facets and nanocubes/nanobars enclosed by {l_brace}100{r_brace} facets by adding sodium citrate (Na{sub 3}CA) and poly(vinyl pyrrolidone) (PVP), respectively, as a capping agent while all other parameters were kept the same. This research not only offers new insights into the role played by a capping agent in shape-controlled synthesis but also provides, for the first time, Ag octahedrons as small as 40 nm in edge length for optical and spectroscopic studies.

  3. Active Nanomaterials to Meet the Challenge of Dental Pulp Regeneration.

    Science.gov (United States)

    Keller, Laetitia; Offner, Damien; Schwinté, Pascale; Morand, David; Wagner, Quentin; Gros, Catherine; Bornert, Fabien; Bahi, Sophie; Musset, Anne-Marie; Benkirane-Jessel, Nadia; Fioretti, Florence

    2015-11-05

    The vitality of the pulp is fundamental to the functional life of the tooth. For this aim, active and living biomaterials are required to avoid the current drastic treatment, which is the removal of all the cellular and molecular content regardless of its regenerative potential. The regeneration of the pulp tissue is the dream of many generations of dental surgeons and will revolutionize clinical practices. Recently, the potential of the regenerative medicine field suggests that it would be possible to achieve such complex regeneration. Indeed, three crucial steps are needed: the control of infection and inflammation and the regeneration of lost pulp tissues. For regenerative medicine, in particular for dental pulp regeneration, the use of nano-structured biomaterials becomes decisive. Nano-designed materials allow the concentration of many different functions in a small volume, the increase in the quality of targeting, as well as the control of cost and delivery of active molecules. Nanomaterials based on extracellular mimetic nanostructure and functionalized with multi-active therapeutics appear essential to reverse infection and inflammation and concomitantly to orchestrate pulp cell colonization and differentiation. This novel generation of nanomaterials seems very promising to meet the challenge of the complex dental pulp regeneration.

  4. Characterization of Human Dental Pulp Tissue Under Oscillatory Shear and Compression.

    Science.gov (United States)

    Ozcan, Burak; Bayrak, Ece; Erisken, Cevat

    2016-06-01

    Availability of material as well as biological properties of native tissues is critical for biomaterial design and synthesis for regenerative engineering. Until recently, selection of biomaterials and biomolecule carriers for dental pulp regeneration has been done randomly or based on experience mainly due to the absence of benchmark data for dental pulp tissue. This study, for the first time, characterizes the linear viscoelastic material functions and compressive properties of human dental pulp tissue harvested from wisdom teeth, under oscillatory shear and compression. The results revealed a gel-like behavior of the pulp tissue over the frequency range of 0.1-100 rps. Uniaxial compression tests generated peak normal stress and compressive modulus values of 39.1 ± 20.4 kPa and 5.5 ± 2.8 kPa, respectively. Taken collectively, the linear viscoelastic and uniaxial compressive properties of the human dental pulp tissue reported here should enable the better tailoring of biomaterials or biomolecule carriers to be employed in dental pulp regeneration.

  5. A prospective study of the incidence of asymptomatic pulp necrosis following crown preparation.

    Science.gov (United States)

    Kontakiotis, E G; Filippatos, C G; Stefopoulos, S; Tzanetakis, G N

    2015-06-01

    To determine the incidence of asymptomatic pulp necrosis following crown preparation as well as the positive predictive value of the electric pulp testing. A total of 120 teeth with healthy pulps scheduled to receive fixed crowns (experimental teeth) were included. Teeth were divided into two groups according to the preoperative crown condition (intact teeth and teeth with preoperative caries, restorations or crowns) and into four groups according to tooth type (maxillary anterior teeth, maxillary posterior teeth, mandibular anterior teeth and mandibular posterior teeth). Experimental and control teeth were submitted to electric pulp testing on three different occasions before treatment commencement (stage 0), at the impression making session (stage 1) and just before the final cementation of the crown (stage 2). Teeth that were considered to contain necrotic pulps were submitted to root canal treatment. Upon access, absence of bleeding was considered as a confirmation of pulp necrosis. Data were analysed using bivariate (chi-square) and multivariate analysis (logistic regression). All reported probability values (P-values) were based on two-sided tests and compared to a significance level of 5%. The overall incidence of pulp necrosis was 9%. Intact teeth had a significantly lower incidence of pulp necrosis (5%) compared with preoperatively structurally compromised teeth (13%) [(OR: 9.113, P = 0.035)]. No significant differences were found amongst the four groups with regard to tooth type (P = 0.923). The positive predictive value of the electric pulp testing was 1.00. The incidence of asymptomatic pulp necrosis of teeth following crown preparation is noteworthy. The presence of preoperative caries, restorations or crowns of experimental teeth correlated with a significantly higher incidence of pulp necrosis. Electric pulp testing remains a useful diagnostic instrument for determining the pulp condition. © 2014 International Endodontic Journal. Published by

  6. pi-Dimers of end-capped oligopyrrole cation radicals

    NARCIS (Netherlands)

    Haare, van J.A.E.H.; Groenendaal, L.; Havinga, E.E.; Janssen, R.A.J.; Meijer, E.W.

    1996-01-01

    In two consecutive one-electron oxidations, oligopyrroles substituted with phenyl capping groups (PhPynPh, n = 2–4) can be oxidized reversibly to give stable cation radicals and dications. Spectroelectrochemical studies give direct evidence that diamagnetic p-dimers of cation radicals are formed in

  7. Local myogenic pulp-derived cell injection enhances craniofacial muscle regeneration in vivo.

    Science.gov (United States)

    Jung, J E; Song, M J; Shin, S; Choi, Y J; Kim, K H; Chung, C J

    2017-02-01

    To enhance myogenic differentiation in pulp cells isolated from extracted premolars by epigenetic modification using a DNA demethylation agent, 5-aza-2'-deoxycytidine (5-Aza), and to evaluate the potent stimulatory effect of 5-Aza-treated pulp cell injection for craniofacial muscle regeneration in vivo. Pulp cells were isolated from premolars extracted for orthodontic purposes from four adults (age range, 18-22.1 years). Levels of myogenic differentiation and functional contraction response in vitro were compared between pulp cells with or without pre-treatment of 5-Aza. Changes in muscle regeneration in response to green fluorescent protein (GFP)-labelled myogenic pulp cell injection in vivo were evaluated using a cardiotoxin (CTX)-induced muscle injury model of the gastrocnemius as well as the masseter muscle in mice. Pre-treatment of 5-Aza in pulp cells stimulated myotube formation, myogenic differentiation in terms of desmin and myogenin expression, and the level of collagen gel contraction. The local injection of 5-Aza pre-treated myogenic pulp cells was engrafted into the host tissue and indicated signs of enhanced muscle regeneration in both the gastrocnemius and the masseter muscles. The epigenetic modification of pulp cells from extracted premolars and the local injection of myogenic pulp cells may stimulate craniofacial muscles regeneration in vivo. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  8. Can Acceptable Pulp be Obtained from Eucalyptus globulus Wood Chips after Hemicellulose Extraction?

    OpenAIRE

    Francisco López; M. Trinidad García; Vicente Mena; J. Mauricio Loaiza; Minerva A. M. Zamudio; Juan C. García

    2014-01-01

    This study investigates the operating conditions used in the soda-anthraquinone pulping of Eucalyptus globulus wood after autohydrolysis pretreatment on the yield, kappa number, and brightness of the resulting unbleached pulp. Moreover, strength-related properties of the resulting handsheets was examined to identify the best pulping conditions and compare the outcome with that of a conventional soda-anthraquinone pulping process. The paper strength properties of the pulp were similar to or be...

  9. Structure-function relationships in the Na,K-ATPase. cap alpha. subunit: site-directed mutagenesis of glutamine-111 to arginine and asparagine-122 to aspartic acid generates a ouabain-resistant enzyme

    Energy Technology Data Exchange (ETDEWEB)

    Price, E.M.; Lingrel, J.B.

    1988-11-01

    Na,K-ATPases from various species differ greatly in their sensitivity to cardiac glycosides such as ouabain. The sheep and human enzymes are a thousand times more sensitive than the corresponding ones from rat and mouse. To define the region of the ..cap alpha..1 subunit responsible for this differential sensitivity, chimeric cDNAs of sheep and rat were constructed and expressed in ouabain-sensitive HeLa cells. The construct containing the amino-terminal half of the rat ..cap alpha..1 subunit coding region and carboxyl-terminal half of the sheep conferred the ouabain-resistant phenotype to HeLa cells while the reverse construct did not. This indicates that the determinants involved in ouabain sensitivity are located in the amino-terminal half of the Na,K-ATPase ..cap alpha.. subunit. By use of site-directed mutagenesis, the amino acid sequence of the first extracellular domain (H1-H2) of the sheep ..cap alpha..1 subunit was changed to that of the rat. When expressed in HeLa cells, this mutated sheep ..cap alpha..1 construct, like the rat/sheep chimera, was able to confer ouabain resistance to these cells. Furthermore, similar results were observed when HeLa cells were transfected with a sheep ..cap alpha..1 cDNA containing only two amino acid substitutions. The resistant cells, whether transfected with the rat ..cap alpha..1 cDNA, the rat/sheep chimera, or the mutant sheep ..cap alpha..1 cDNAs, exhibited identical biochemical characteristics including ouabain-inhibitable cell growth, /sup 86/Rb/sup +/ uptake, and Na,K-ATPase activity. These results demonstrate that the presence of arginine and aspartic acid on the amino end and carboxyl end, respectively, of the H1-H2 extracellular domain of the Na,K-ATPase ..cap alpha.. subunit together is responsible for the ouabain-resistant character of the rat enzyme and the corresponding residues in the sheep ..cap alpha..1 subunit (glutamine and asparagine) are somehow involved in ouabain binding.

  10. A survey of present levels of radiocesium in Swedish pulp mill liquors and the implications for wood radiocesium transfer factors

    International Nuclear Information System (INIS)

    Ravila, A.; Holm, E.

    1998-01-01

    Assessments of the impact from radioactive fallout on the pulp industry and the accumulation processes for radionuclides in such industries, using wood and water as primary raw materials, have been conducted in previous studies. Hitherto, there is a general lack of data on present levels of radioactivity in pulp mill liquors apart from the few mills studied by [NILSSON (1992)] [RAVILA AND HOLM (1992)] [MANJON ET AL. (1996) and [KROSSHAVN ET AL. (1997)]. A survey was therefore initiated to describe and compare the present activity levels in pulp mill liquors from various mill locations in Sweden. The 137 Cs activity in pulp mill liquors were compared with the mill location and the deposition pattern of the Chernobyl fallout and nuclear weapons fallout. The large input of wood (approx. 2500 m 3 day -1 ) to an average Nordic Kraft mill and the relatively long-term retention time for radiocesium in the Kraft mill recovery cycle enables representative sampling of substances directly related to the activity concentration in wood

  11. Tropical Fruit Pulps: Processing, Product Standardization and Main Control Parameters for Quality Assurance

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo de Farias Silva

    2017-05-01

    Full Text Available ABSTRACT Fruit pulp is the most basic food product obtained from fresh fruit processing. Fruit pulps can be cold stored for long periods of time, but they also can be used to fabricate juices, ice creams, sweets, jellies and yogurts. The exploitation of tropical fruits has leveraged the entire Brazilian fruit pulp sector due mainly to the high acceptance of their organoleptic properties and remarkable nutritional facts. However, several works published in the last decades have pointed out unfavorable conditions regarding the consumption of tropical fruit pulps. This negative scenario has been associated with unsatisfactory physico-chemical and microbiological parameters of fruits pulps as outcomes of little knowledge and improper management within the fruit pulp industry. There are protocols for delineating specific identity and quality standards (IQSs and standardized good manufacturing practices (GMP for fruit pulps, which also embrace standard operating procedures (SOPs and hazard analysis and critical control points (HACCP, although this latter is not considered mandatory by the Brazilian legislation. Unfortunately, the lack of skilled labor, along with failures in complying established protocols have impaired quality of fruit pulps. It has been necessary to collect all information available with the aim to identify the most important hazards within fruit pulp processing lines. Standardizing methods and practices within the Brazilian fruit pulp industry would assurance high quality status to tropical fruit pulps and the commercial growth of this vegetal product towards international markets.

  12. Microwave-assisted deposition of silver nanoparticles on bamboo pulp fabric through dopamine functionalization

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Linghui [College of Light Industry, Textile and Food Engineering, Sichuan University, No.24 South Section 1, Yihuan Road, Chengdu (China); Guo, Ronghui, E-mail: ronghuiguo214@126.com [College of Light Industry, Textile and Food Engineering, Sichuan University, No.24 South Section 1, Yihuan Road, Chengdu (China); Lan, Jianwu [College of Light Industry, Textile and Food Engineering, Sichuan University, No.24 South Section 1, Yihuan Road, Chengdu (China); Jiang, Shouxiang [Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong (China); Lin, Shaojian [Institute for Technical and Macromolecular Chemistry, University of Hamburg Bundesstrasse 45, D-20146 Hamburg (Germany)

    2016-11-15

    Highlights: • Silver nanoparticles were synthesized on bamboo pulp fabric using dopamine as an adhesive and reducing agent under microwave radiation. • Silver coated bamboo pulp fabric modified with dopamine has good UV protection and hydrophobic property. • Silver nanoparticles can be strongly fixed on dopamine modified bamboo pulp fabric. - Abstract: Silver nanoparticles were synthesized on bamboo pulp fabric with dopamine as the adhesive and reducing agent under microwave radiation. The silver nanoparticle coated bamboo pulp fabrics were characterized by X-ray photoelectron spectroscopy, scanning electron microscope and X-ray diffraction. Ultraviolet (UV) protection, color and water contact angles of the silver nanoparticle coated bamboo pulp fabrics were evaluated. In addition, the influences of concentrations of dopamine and treatment time on color strength (K/S values) of the silver nanoparticle coated fabric were investigated. Fastness to washing was employed to evaluate the adhesive strength between the silver coating and the bamboo pulp fabric modified with dopamine. The results show that the dopamine modified bamboo pulp fabric is evenly covered with silver nanoparticles. The silver nanoparticle coated bamboo pulp fabric modified with dopamine shows the excellent UV protection with an ultraviolet protection factor of 157.75 and the hydrophobicity with a water contact angle of 132.4°. In addition, the adhesive strength between the silver nanoparticles and bamboo pulp fabric is significantly improved. Silver nanoparticles coating on bamboo pulp fabric modified with dopamine is environmentally friendly, easy to carry out and highly efficient.

  13. ON THE RECOVERY OF HEMICELLULOSE BEFORE KRAFT PULPING

    Directory of Open Access Journals (Sweden)

    Carlos Vila,

    2012-07-01

    Full Text Available To assess the feasibility of implementing hemicellulose recovery stages in kraft mills, Eucalyptus globulus wood samples were subjected to aqueous treatments with hot, compressed water (autohydrolysis processing to achieve partial dissolution of xylan. Autohydrolyzed solids were subjected to kraft pulping under selected conditions to yield a pulp of low kappa number, and to an optimized TCF bleaching sequence made up of three stages (alkaline oxygen delignification, chelating, and pressurized hydrogen peroxide, with minimized additions of pulping and bleaching chemicals. The final product had a relatively low kappa number (1.4, 641 mL/g ISO intrinsic viscosity, and 86.4% brightness.

  14. Enzyme Enhanced Protein Recovery from Green Biomass Pulp

    DEFF Research Database (Denmark)

    Dotsenko, Gleb; Lange, Lene

    2017-01-01

    of local protein resources based on upgrade from e.g. green plant biomass. In present work we consider different strategies for protein recovery from white clover and ryegrass screw press pulps, using aqueous extraction, as well as carbohydrases and proteases enhanced extraction. Protein recovery...... in these studies was determined as a yield of solubilized protein with regard to the total protein in a screw press pulp. Aqueous extraction at pH 8.0 resulted in approx. 40 % protein recovery, while proteases application (Savinase 16.0L, Novozymes) enabled twice higher protein yield. Application of plant cell...... pulp proteolyzates, generated by Savinase 16.0L protease....

  15. 78 FR 38877 - Kraft Pulp Mills NSPS Review

    Science.gov (United States)

    2013-06-28

    ... ENVIRONMENTAL PROTECTION AGENCY 40 CFR Part 60 [EPA-HQ-OAR-2012-0640] FRL-9829-3 RIN 2060-AR64 Kraft Pulp Mills NSPS Review AGENCY: Environmental Protection Agency (EPA). ACTION: Proposed rule... comments on the May 23, 2013, proposed rule titled, ``Kraft Pulp Mills NSPS Review'' is being extended by...

  16. The pharmaceutical vial capping process: Container closure systems, capping equipment, regulatory framework, and seal quality tests.

    Science.gov (United States)

    Mathaes, Roman; Mahler, Hanns-Christian; Buettiker, Jean-Pierre; Roehl, Holger; Lam, Philippe; Brown, Helen; Luemkemann, Joerg; Adler, Michael; Huwyler, Joerg; Streubel, Alexander; Mohl, Silke

    2016-02-01

    Parenteral drug products are protected by appropriate primary packaging to protect against environmental factors, including potential microbial contamination during shelf life duration. The most commonly used CCS configuration for parenteral drug products is the glass vial, sealed with a rubber stopper and an aluminum crimp cap. In combination with an adequately designed and controlled aseptic fill/finish processes, a well-designed and characterized capping process is indispensable to ensure product quality and integrity and to minimize rejections during the manufacturing process. In this review, the health authority requirements and expectations related to container closure system quality and container closure integrity are summarized. The pharmaceutical vial, the rubber stopper, and the crimp cap are described. Different capping techniques are critically compared: The most common capping equipment with a rotating capping plate produces the lowest amount of particle. The strength and challenges of methods to control the capping process are discussed. The residual seal force method can characterize the capping process independent of the used capping equipment or CCS. We analyze the root causes of several cosmetic defects associated with the vial capping process. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Using renewable sources in the pulp and paper mills

    International Nuclear Information System (INIS)

    Hazi, Aneta; Hazi, Gheorghe

    2007-01-01

    The pulp and paper industry is a major consumer of natural sources (wood) and energy (fossil fuels, electricity) and a significant contributor of pollutant discharges to the environment. In this paper there are presented pulp and paper making process and steam and power generation using renewable sources. This paper includes also an exergy analysis of the steam and power generation process for a pulp and paper mill. Based on the analysis, two sustainability indicators were calculated: the exergetic efficiency and the exergy renewability. (authors)

  18. Characterization of Cypress Wood for Kraft Pulp Production

    Directory of Open Access Journals (Sweden)

    António J. A. Santos

    2014-06-01

    Full Text Available Wood samples of Cupressus arizonica, C. lusitanica, and C. sempervirens were evaluated for chemical, anatomical, and pulp characteristics as raw material for pulp production. Two 17-year-old trees per species were harvested, and wood samples were taken at a height of 2 m. Wood chips from Pinus pinaster (Portugal and P. sylvestris (Finland were used as references. C. arizonica differed from C. lusitanica and C. sempervirens with significantly lower (p < 0.05 tracheid diameter and wall thickness in the earlywood. The total extractives contents were 3.9%, 3.3%, and 2.5% for C. lusitanica, C. sempervirens, and C. arizonica, respectively, lower than the 5.1% for P. pinaster and 4.5% for P. sylvestris. Klason lignin content ranged from 33.0 to 35.6%, higher than the 28.0 to 28.7% for the pinewoods. The kraft pulp yields for C. arizonica, C. lusitanica, and C. sempervirens were 37.7%, 36.7%, and 38.7%, respectively, with kappa numbers of 32.0, 31.6, and 28.7, respectively; the yield values were 40.8% and 42.8%, with kappa numbers of 23.4 and 21.0, for P. pinaster and P. sylvestris, respectively. The cypress species are clearly different from pine in relation to wood pulping behavior. Among the cypress, C. sempervirens provided the best pulping results.

  19. Effect of Different Moisture Absorbents on Silage Fermentation Quality of Wet Potato Pulp

    Directory of Open Access Journals (Sweden)

    Daryoush Alipour

    2016-11-01

    .01. The higher value of partitioning factor shows the better quality of feedstuff and reveals that more fermented organic matter directed towards microbial biomass rather than production of volatile fatty acids. Conclusion Because of high moisture content of potato pulp, it is suggested to ensiling this by-product for using as ruminant feedstuff. In this study, rejected raisin was the best moisture absorbent. The results showed that treatments containing rejected raisin, pomegranate peel, pedicles and rachis were suitable absorbent in ensilage process because of lower pH, higher lactic acid production and better quality of silage, respectively.

  20. Research on Wheat Straw Pulping with Ionic Liquid 1-Ethyl-3-Methylimidazole Bromide

    Directory of Open Access Journals (Sweden)

    Wei Song

    2016-12-01

    Full Text Available In this paper, the pulping process of wheat straw using ionic liquid 1-ethyl-3-methylimidazolium bromide ([Emim]Br as the digestion liquor is presented. The influence of pulping conditions on the pulp yield are analysed by single-factor and orthogonal experiments, and optimum pulping conditions are obtained. The average pulp yield reaches 44 %, and the average recovery rate of ionic liquid is 93.5 %. The XRD pattern shows no obvious change in the crystal structure of the wheat straw cellulose. Additionally, the SEM image illustrates that there are many fine fibres in the pulp and the spaces between the fibres are large.

  1. Structure of a two-CAP-domain protein from the human hookworm parasite Necator americanus

    Energy Technology Data Exchange (ETDEWEB)

    Asojo, Oluwatoyin A., E-mail: oasojo@unmc.edu [Pathology and Microbiology Department, 986495 Nebraska Medical Center, Omaha, NE 68198-6495 (United States)

    2011-05-01

    The first structure of a two-CAP-domain protein, Na-ASP-1, from the major human hookworm parasite N. americanus refined to a resolution limit of 2.2 Å is presented. Major proteins secreted by the infective larval stage hookworms upon host entry include Ancylostoma secreted proteins (ASPs), which are characterized by one or two CAP (cysteine-rich secretory protein/antigen 5/pathogenesis related-1) domains. The CAP domain has been reported in diverse phylogenetically unrelated proteins, but has no confirmed function. The first structure of a two-CAP-domain protein, Na-ASP-1, from the major human hookworm parasite Necator americanus was refined to a resolution limit of 2.2 Å. The structure was solved by molecular replacement (MR) using Na-ASP-2, a one-CAP-domain ASP, as the search model. The correct MR solution could only be obtained by truncating the polyalanine model of Na-ASP-2 and removing several loops. The structure reveals two CAP domains linked by an extended loop. Overall, the carboxyl-terminal CAP domain is more similar to Na-ASP-2 than to the amino-terminal CAP domain. A large central cavity extends from the amino-terminal CAP domain to the carboxyl-terminal CAP domain, encompassing the putative CAP-binding cavity. The putative CAP-binding cavity is a characteristic cavity in the carboxyl-terminal CAP domain that contains a His and Glu pair. These residues are conserved in all single-CAP-domain proteins, but are absent in the amino-terminal CAP domain. The conserved His residues are oriented such that they appear to be capable of directly coordinating a zinc ion as observed for CAP proteins from reptile venoms. This first structure of a two-CAP-domain ASP can serve as a template for homology modeling of other two-CAP-domain proteins.

  2. Structure of a two-CAP-domain protein from the human hookworm parasite Necator americanus

    International Nuclear Information System (INIS)

    Asojo, Oluwatoyin A.

    2011-01-01

    The first structure of a two-CAP-domain protein, Na-ASP-1, from the major human hookworm parasite N. americanus refined to a resolution limit of 2.2 Å is presented. Major proteins secreted by the infective larval stage hookworms upon host entry include Ancylostoma secreted proteins (ASPs), which are characterized by one or two CAP (cysteine-rich secretory protein/antigen 5/pathogenesis related-1) domains. The CAP domain has been reported in diverse phylogenetically unrelated proteins, but has no confirmed function. The first structure of a two-CAP-domain protein, Na-ASP-1, from the major human hookworm parasite Necator americanus was refined to a resolution limit of 2.2 Å. The structure was solved by molecular replacement (MR) using Na-ASP-2, a one-CAP-domain ASP, as the search model. The correct MR solution could only be obtained by truncating the polyalanine model of Na-ASP-2 and removing several loops. The structure reveals two CAP domains linked by an extended loop. Overall, the carboxyl-terminal CAP domain is more similar to Na-ASP-2 than to the amino-terminal CAP domain. A large central cavity extends from the amino-terminal CAP domain to the carboxyl-terminal CAP domain, encompassing the putative CAP-binding cavity. The putative CAP-binding cavity is a characteristic cavity in the carboxyl-terminal CAP domain that contains a His and Glu pair. These residues are conserved in all single-CAP-domain proteins, but are absent in the amino-terminal CAP domain. The conserved His residues are oriented such that they appear to be capable of directly coordinating a zinc ion as observed for CAP proteins from reptile venoms. This first structure of a two-CAP-domain ASP can serve as a template for homology modeling of other two-CAP-domain proteins

  3. The Kraft Pulp And Paper Properties of Sweet Sorghum Bagasse (Sorghum bicolor L Moench

    Directory of Open Access Journals (Sweden)

    Widya Fatriasari

    2015-05-01

    Full Text Available This study investigated the potency of sweet sorghum (Sorghum bicolor bagasse as raw material for pulp and paper using kraft pulping. The effects of alkali and sulfidity loading on kraft pulp and paper properties were also investigated. The pulping condition of the kraft pulp consisted of three levels of alkali loading (17, 19 and 22% and sulfidity loading (20, 22 and 24%. The maximum cooking temperature was 170°C for 4 h with a liquid to wood ratio of 10:1. Kraft pulping of this Numbu bagasse produced good pulp indicated by high screen yield and delignification selectivity with a low Kappa number (< 10. The unbleached pulp sheet produced a superior brightness level and a high burst index. The increase of active alkali loading tended to produce a negative effect on the pulp yield, Kappa number and paper sheet properties. Therefore, it is suggested to use a lower active alkaline concentration.

  4. Capítulo I: Contabilidad de costos - Capítulo II : Costos estimados

    OpenAIRE

    Rojas Medina, Ricardo Alfredo

    2014-01-01

    Me permito poner a su consideración los capítulos I y II del libro titulado: Costos un enfoque administrativo y de gerencia, contenido que fue realizado para mejorar y reestructurar el libro titulado Sistemas de costos un proceso para su implementación, que tuvo gran acogida dado el volumen de consulta y descarga del repositorio de la UN. En la primera unidad se trabaja lo referente a costos para la toma de decisiones, presentado un marco teórico y los fundamentos básicos del costeo direct...

  5. Pulp tissue in sex determination: A fluorescent microscopic study

    Science.gov (United States)

    Nayar, Amit; Singh, Harkanwal Preet; Leekha, Swati

    2014-01-01

    Aims: To determine and compare the reliability of pulp tissue in determination of sex and to analyze whether caries have any effect on fluorescent body test. Materials and Methods: This study was carried on 50 maxillary and mandibular teeth (25 male teeth and 25 female teeth), which were indicated for extraction. The teeth are categorized into 5 groups, 10 each (5 from males and 5 from females) on the basis of caries progression. The pulp cells are stained with quinacrine hydrochloride and observed with fluorescent microscope for fluorescent body. Gender is determined by identification of Y chromosome fluorescence in dental pulp. Results: Fluorescent bodies were found to be more in sound teeth in males as the caries increase the mean percentage of fluorescent bodies observed decreases in males. We also observed the fluorescent spots in females, and the value of the spot increases in female as the caries progresses, thereby giving false positive results in females. Conclusion: Sex determination by fluorescent staining of the Y chromosome is a reliable technique in teeth with healthy pulps or caries with enamel or up to half way of dentin. Teeth with caries involving pulp cannot be used for sex determination. PMID:25125912

  6. In vitro penetration of bleaching agents into the pulp chamber

    DEFF Research Database (Denmark)

    Benetti, Ana Raquel; Valera, M C; Mancini, M N G

    2004-01-01

    To investigate pulp chamber penetration of bleaching agents in teeth following restorative procedures.......To investigate pulp chamber penetration of bleaching agents in teeth following restorative procedures....

  7. NIRS Characterization of Paper Pulps to Predict Kappa Number

    Directory of Open Access Journals (Sweden)

    Ana Moral

    2015-01-01

    Full Text Available Rice is one of the most abundant food crops in the world and its straw stands as an important source of fibres both from an economic and an environmental point of view. Pulp characterization is of special relevance in works involving alternative raw materials, since pulp properties are closely linked to the quality of the final product. One of the analytical techniques that can be used in pulp characterization is near-infrared spectroscopy (NIRS. The use of NIRS has economic and technical advantages over conventional techniques. This paper aims to discuss the convenience of using NIRS to predict Kappa number in rice straw pulps produced under different conditions. We found that the resulting Kappa number can be acceptably estimated by NIRS, as the errors obtained with that method are similar to those found for other techniques.

  8. Catastrophic antiphospholipid syndrome (CAPS): Descriptive analysis of 500 patients from the International CAPS Registry.

    Science.gov (United States)

    Rodríguez-Pintó, Ignasi; Moitinho, Marta; Santacreu, Irene; Shoenfeld, Yehuda; Erkan, Doruk; Espinosa, Gerard; Cervera, Ricard

    2016-12-01

    To analyze the clinical and immunologic manifestations of patients with catastrophic antiphospholipid syndrome (CAPS) from the "CAPS Registry". The demographic, clinical and serological features of 500 patients included in the website-based "CAPS Registry" were analyzed. Frequency distribution and measures of central tendency were used to describe the cohort. Comparison between groups regarding qualitative variables was undertaken by chi-square or Fisher exact test while T-test for independent variables was used to compare groups regarding continuous variables. 500 patients (female: 343 [69%]; mean age 38±17) accounting for 522 episodes of CAPS were included in the analysis. Forty percent of patients had an associated autoimmune disease, mainly systemic lupus erythematosus (SLE) (75%). The majority of CAPS episodes were triggered by a precipitating factor (65%), mostly infections (49%). Clinically, CAPS was characterized by several organ involvement affecting kidneys (73%), lungs (60%), brain (56%), heart (50%), and skin (47%). Lupus anticoagulant, IgG anticardiolipin and IgG anti-β2-glycprotein antibodies were the most often implicated antiphospholipid antibodies (83%, 81% and 78% respectively). Mortality accounted for 37% of episodes of CAPS. Several clinical differences could be observed based on the age of presentation and its association to SLE. Those cases triggered by a malignancy tended to occur in older patients, while CAPS episodes in young patients were associated with an infectious trigger and peripheral vessels involvement. Additionally, CAPS associated with SLE were more likely to have severe cardiac and brain involvement leading to a higher mortality (48%). Although the presentation of CAPS is characterized by multiorgan thrombosis and failure, clinical differences among patients exist based on age and underlying chronic diseases, e.g. malignancy and SLE. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. An overview of the dental pulp: its functions and responses to injury.

    Science.gov (United States)

    Yu, C; Abbott, P V

    2007-03-01

    The dental pulp is a unique tissue and its importance in the long-term prognosis of the tooth is often ignored by clinicians. It is unique in that it resides in a rigid chamber which provides strong mechanical support and protection from the microbial rich oral environment. If this rigid shell loses its structural integrity, the pulp is under the threat of the adverse stimuli from the mouth, such as caries, cracks, fractures and open restoration margins, all of which provide pathways for micro-organisms and their toxins to enter the pulp. The pulp initially responds to irritation by becoming inflamed and, if left untreated, this will progress to pulp necrosis and infection. The inflammation will also spread to the surrounding alveolar bone and cause periapical pathosis. The magnitude of pulp-related problems should not be underestimated since their most serious consequence is oral sepsis, which can be life threatening, and hence correct diagnosis and management are essential. Clinicians must have a thorough understanding of the physiological and pathological features of the dental pulp as well as the biological consequences of treatment interventions.

  10. Active Nanomaterials to Meet the Challenge of Dental Pulp Regeneration

    Directory of Open Access Journals (Sweden)

    Laetitia Keller

    2015-11-01

    Full Text Available The vitality of the pulp is fundamental to the functional life of the tooth. For this aim, active and living biomaterials are required to avoid the current drastic treatment, which is the removal of all the cellular and molecular content regardless of its regenerative potential. The regeneration of the pulp tissue is the dream of many generations of dental surgeons and will revolutionize clinical practices. Recently, the potential of the regenerative medicine field suggests that it would be possible to achieve such complex regeneration. Indeed, three crucial steps are needed: the control of infection and inflammation and the regeneration of lost pulp tissues. For regenerative medicine, in particular for dental pulp regeneration, the use of nano-structured biomaterials becomes decisive. Nano-designed materials allow the concentration of many different functions in a small volume, the increase in the quality of targeting, as well as the control of cost and delivery of active molecules. Nanomaterials based on extracellular mimetic nanostructure and functionalized with multi-active therapeutics appear essential to reverse infection and inflammation and concomitantly to orchestrate pulp cell colonization and differentiation. This novel generation of nanomaterials seems very promising to meet the challenge of the complex dental pulp regeneration.

  11. Studies of lignin transformation in polyoxometalate (POM) bleaching of kraft pulp

    Science.gov (United States)

    Biljana Bujanovic; Richard S. Reiner; Kolby C. Hirth; Sally A. Ralph; Rajai H. Atalla

    2005-01-01

    In order to elucidate changes occurring in lignin during polyoxometalate delignification of kraft pulp, residual lignins of a series of POM- delignified kraft pulps of decreasing kappa number were isolated and characterized. Oxidative treatment of commercial unbleached kraft pulp was performed using complex POM solutions containing the active [SiVW11O40]anion. For...

  12. Angular correlation between short-range. cap alpha. particles and. gamma. quanta

    Energy Technology Data Exchange (ETDEWEB)

    Kul' chitskii, L A; Latyshev, G D; Bulyginskii, D G

    1949-01-01

    Chang (Phys. Rev. 69, 60(1946); 70, 632(1946)) has found that the intensities of short-range ..cap alpha.. rays of Po and Ra are considerably higher than the values given by the Geiger-Nuttall law. This can be explained by assuming surface vibrations of ..cap alpha..-radioactive nuclei, which produce deformations and corresponding lowerings of the potential barrier in certain directions. In this case an angular correlation should exist between the short-range ..cap alpha.. ray and the accompanying ..gamma.. quantum. The authors checked this conclusion by applying the coincidence method to the ..cap alpha.. and ..gamma.. radiations of a mixture of RdTh (/sup 228/Th) and ThC (/sup 212/Bi). Maxima of coincidence numbers occur at angles 45 and 135 deg., with lesser maxima at 0 and 180 deg. Theoretical considerations show that in cases (like the one investigated) where the nuclear spin before and after the ..cap alpha.. and ..gamma.. emissions is zero, the angular correlations are uniquely determined whatever the deformation caused by the vibration; in other cases, the correlation depends on the kind of deformation. Therefore, it would be interesting to investigate the case of Pa, whose nuclear spin is not zero and the decay exhibits intensive groups of short-range ..cap alpha.. particles.

  13. Catalase activity in healthy and inflamed pulp tissues of permanent teeth in young people.

    Science.gov (United States)

    Topcu, Kmc; Kırıcı, D Ö; Evcil, M S

    2016-01-01

    To evaluate catalase (CAT, EC 1.11.1.6) activity in healthy and inflamed dental pulp of young patient's teeth and to investigate if an active defense system oxidizing agents is present as a response to bacterial invasion. Twenty young patients between 15 and 25 ages, who were diagnosed to be healthy, were the source of the pulp tissue. The situation of the dental pulps was evaluated using clinical and radiographic assessments. The patients were divided two groups from healthy, and inflamed pulp tissues were obtained; each participant provided one pulp tissue specimens. The specimens were collected during endodontic treatment or by longitudinally grooving and splitting the teeth (if extracted). Catalase activity was determined through spectrophotometric methods and an independent sample t-test assessed the significance of differences between the groups. There was statistically a difference between healthy pulp tissue and inflamed pulp tissue (P catalase activity of healthy group was significantly lower than inflamed pulp groups. The present study has shown that a significant increase in catalase activity is determined in inflamed dental pulps, which is due to pulpitis in comparison to healthy dental pulp.

  14. Characterisation of Arabica Coffee Pulp - Hay from Kintamani - Bali as Prospective Biogas Feedstocks

    Directory of Open Access Journals (Sweden)

    Hendroko Setyobudi Roy

    2018-01-01

    Full Text Available The huge amount of coffee pulp waste is an environmental problem. Anaerobic fermentation is one of the alternative solutions. However, availability of coffee pulp does not appear for year-round, whereas biogas needs continuous feedstocks for digester stability. This research uses coffee pulp from Arabica Coffee Factory at Mengani, Kintamani, Bali–Indonesia. The coffee pulp was transformed into coffee pulp-hay product by sun drying for preservations to extend the raw materials through the year. Characterization of coffee pulp-hay was conducted after to keep for 15 mo for review the prospect as biogas feedstocks. Several parameters were analyzed such as C/N ratio, volatile solids, carbohydrate, protein, fat, lignocellulose content, macro-micro nutrients, and density. The review results indicated that coffee pulp-hay is prospective raw material for biogas feedstock. This well-proven preservation technology was able to fulfill the continuous supply. Furthermore, some problems were found in the recent preliminary experiment related to the density and fungi growth in the conventional laboratory digester. Further investigation was needed to implement the coffee pulp – hay as biogas feedstocks.

  15. Advanced Scaffolds for Dental Pulp and Periodontal Regeneration.

    Science.gov (United States)

    Bottino, Marco C; Pankajakshan, Divya; Nör, Jacques E

    2017-10-01

    No current therapy promotes root canal disinfection and regeneration of the pulp-dentin complex in cases of pulp necrosis. Antibiotic pastes used to eradicate canal infection negatively affect stem cell survival. Three-dimensional easy-to-fit antibiotic-eluting nanofibers, combined with injectable scaffolds, enriched or not with stem cells and/or growth factors, may increase the likelihood of achieving predictable dental pulp regeneration. Periodontitis is an aggressive disease that impairs the integrity of tooth-supporting structures and may lead to tooth loss. The latest advances in membrane biomodification to endow needed functionalities and technologies to engineer patient-specific membranes/constructs to amplify periodontal regeneration are presented. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. Human dental pulp stem cells derived from cryopreserved dental pulp tissues of vital extracted teeth with disease demonstrate hepatic-like differentiation.

    Science.gov (United States)

    Chen, Y K; Huang, Anderson H C; Chan, Anthony W S; Lin, L M

    2016-06-01

    Reviewing the literature, hepatic differentiation of human dental pulp stem cells (hDPSCs) from cryopreserved dental pulp tissues of vital extracted teeth with disease has not been studied. This study is aimed to evaluate the hypothesis that hDPSCs from cryopreserved dental pulp tissues of vital extracted teeth with disease could possess potential hepatic differentiation. Forty vital extracted teeth with disease recruited for hDPSCs isolation, stem cell characterization and hepatic differentiation were randomly and equally divided into group A (liquid nitrogen-stored dental pulp tissues) and group B (freshly derived dental pulp tissues). Samples of hDPSCs isolated from groups A and B but without hepatic growth factors formed negative controls. A well-differentiated hepatocellular carcinoma cell line was employed as a positive control. All the isolated hDPSCs from groups A and B showed hepatic-like differentiation with morphological change from a spindle-shaped to a polygonal shape and normal karyotype. Differentiated hDPSCs and the positive control expressed hepatic metabolic function genes and liver-specific genes. Glycogen storage of differentiated hDPSCs was noted from day 7 of differentiation-medium culture. Positive immunofluorescence staining of low-density lipoprotein and albumin was observed from day 14 of differentiation-medium culture; urea production in the medium was noted from week 6. No hepatic differentiation was observed for any of the samples of the negative controls. We not only demonstrated the feasibility of hepatic-like differentiation of hDPSCs from cryopreserved dental pulp tissues of vital extracted teeth with disease but also indicated that the differentiated cells possessed normal karyotype and were functionally close to normal hepatic-like cells. Copyright © 2013 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  17. The rebirth of the cervical cap.

    Science.gov (United States)

    Cappiello, J D; Grainger-Harrison, M

    1981-01-01

    In an effort to dispel myths surrounding the cervical cap, the historical and political factors affecting the cap's use in the U.S. are described. Clinical aspects of cap fitting are also included. The cervical cap has found only limited acceptance in the U.S. Skepticisms on the part of physicians may be the result of 2 factors: confusion of the cervical cap with intracervical devices used for artificial insemination and confusion with stem pessaries; and the lack of clinical research and statistical evaluation of efficacy rates. The latter factor prompted Tietze et al. to conduct the only U.S. statistical study of the cap in 1953. Of the 143 women studied, the pregnancy rate was 7.6/100 years of use. Of the 28 unplanned pregnancies, 6 were related to faulty technique or omission of a spermicide and 10 were instances of admittedly irregular use. When these failures are omitted, the theoretical effectiveness rate is about 98%. Some practitioners are concerned about an increased incidence of cervical erosion with cap use. Possibly currently conducted studies will show that cap and spermicide users have a lower incidence of cervical erosion than women using no contraceptive method. Study findings suggest that the cervical cap may afford protection without any spermicidal supplement, but the use of spermicides continues to be recommended to clients. Advantages of the cervical cap include the following: it can be left in place longer than a diaphragm without additional applications of spermicide in the vagina; and the insertion of the cap is unrelated to the time of intercourse. Despite research on toleration of the cap for 3 weeks at a time, it is recommended that the cap be worn for only a few days at a time. At this time there are no manufacturers of cervical caps for contraceptive use in the U.S. The cap is now being imported from England and it costs $6.00. A factor that has made the cap unpopular with many physicians is the lengthy time required for fitting. An

  18. CAP payments and agricultural GHG emissions in Italy. A farm-level assessment.

    Science.gov (United States)

    Coderoni, Silvia; Esposti, Roberto

    2018-06-15

    The Common Agricultural Policy (CAP) is an important external driver of European agricultural production. Nowadays and in its envisioned future structure post-2020, the CAP has among its major objectives tackling climate change, for what concerns both adaptation and mitigation strategies. However, little is known about the link between past CAP reforms and agricultural greenhouse gases (GHG) emissions. This paper investigates the possible role played by the Fischler Reform (FR) on the agricultural GHG emissions at the farm level. The FR represents a major CAP reform for which data availability allows an ex-post analysis about its actual impacts. The empirical analysis concerns a balanced panel of 6542 Italian Farm Accountancy Data Network observed over years the 2003-2007. Multinomial Logit models are estimated in sequence to express how the farm-level production choices, and the respective emissions, vary over time also in response to CAP expenditure. Results suggest that CAP expenditure had a role in the evolution of the farm-level emissions, though the direction of this effect may differ across farms and deserves further investigation. Copyright © 2018 Elsevier B.V. All rights reserved.

  19. Action of nitric oxide on healthy and inflamed human dental pulp tissue.

    Science.gov (United States)

    da Silva, Leopoldo Penteado Nucci; Issa, João Paulo Mardegan; Del Bel, Elaine Aparecida

    2008-10-01

    Irreversible pulpitis has been associated with pain and an increase in the number of pulp inflammatory cells. Based on the action of nitric oxide (NO) elsewhere, NO may possibly participate in the sensory and autonomic innervation of the dental pulp, and may influence local inflammatory responses. The purpose of this study was to analyze normal and inflamed human dental pulp for the presence of NADPH-diaphorase (NADPH-d), as an index of NO system activity. Six non-carious second premolar pulp tissue samples were obtained from young patients who required extractions for orthodontic reasons and six inflamed samples were obtained from symptomatic carious second premolars clinically diagnosed with irreversible pulpitis. Pulp tissue was carefully removed, fixed by immersion in a cold 4% PFA buffered solution for 120 min, rinsed in cold phosphate buffer, and quickly-frozen for cryostat sectioning. Pulp tissue was sectioned perpendicularly to the vertical axis of the tooth at 20 microm and processed for histochemistry. Sections of each specimen were stained with hematoxylin-eosin and other sections were subjected to histochemical NADPH-d detection. Results indicated the presence of NADPH reactivity within the pulps of both normal and carious teeth. In the normal teeth NADPH-d activity was detected in a small number of vascular endothelial cells and fibroblasts. The inflammatory response of the pulp from carious premolars was detected in connective tissue by the presence of an increased number of fibroblasts, angioblasts and collagen fibers. It was possible to determine the extent of odontoblast reactivity since the odontoblast layer was usually absent in these split-peel preparations. There were no obvious signs of stained pulpal nerve fibers. Overall NADPH-d staining was significantly more intense within inflamed pulp tissues compared to normal healthy samples (Mann-Whitney test, pfunctions of NO in human dental pulp in pathophysiological situations.

  20. Factors affecting direction and strength of patient preferences for treatment of molar teeth with nonvital pulps.

    Science.gov (United States)

    Vernazza, C R; Steele, J G; Whitworth, J M; Wildman, J R; Donaldson, C

    2015-12-01

    To elicit the factors affecting willingness to pay (WTP) values for the preferred options of participants for dealing with a molar tooth with a nonvital pulp, a common but difficult problem. A total of 503 patients were recruited from dental practices in the North East of England and interviewed. Their preferred treatment option for a molar tooth with a nonvital pulp (endodontics, extraction and various prosthetic restorative options) and WTP for this preferred option were elicited. Factors affecting preferred option and WTP were analysed using econometric modelling. Overall, 53% of the sample wished to save the tooth with a mean WTP of £373. The variance in WTP was high. Of those opting for extraction, the majority chose to leave a gap or have an implant. The preferred option was influenced by previous treatment experience. WTP was only influenced by having a low income. The high level of variance in WTP and its relatively unpredictable nature pose difficult questions for policy makers trying to ensure the delivery of an equitable service. For dentists, it is important not to make assumptions about patient preference and strength of preference when making decisions. Ideally, WTP values should be considered alongside effectiveness data, and those on costs, in policy making. © 2014 International Endodontic Journal. Published by John Wiley & Sons Ltd.

  1. Simultaneous production of bio-ethanol and bleached pulp from red algae.

    Science.gov (United States)

    Yoon, Min Ho; Lee, Yoon Woo; Lee, Chun Han; Seo, Yung Bum

    2012-12-01

    The red algae, Gelidium corneum, was used to produce bleached pulp for papermaking and ethanol. Aqueous extracts obtained at 100-140 °C were subjected to saccharification, purification, fermentation, and distillation to produce ethanol. The solid remnants were bleached with chlorine dioxide and peroxide to make pulp. In the extraction process, sulfuric acid and sodium thiosulfate were added to increase the extract yield and to improve de-polymerization of the extracts, as well as to generate high-quality pulp. An extraction process incorporating 5% sodium thiosulfate by dry weight of the algae provided optimal production conditions for the production of both strong pulp and a high ethanol yield. These results suggest that it might be possible to utilize algae instead of trees and starch for pulp and ethanol production, respectively. Copyright © 2012 Elsevier Ltd. All rights reserved.

  2. Use of Coffee Pulp and Minerals for Natural Soil Ameliorant

    Directory of Open Access Journals (Sweden)

    Pujiyanto Pujiyanto

    2007-05-01

    Full Text Available In coffee plantation, solid waste of coffee pulp is usually collected as heap nearby processing facilities for several months prior being used as compost. The practice is leading to the formation of odor and liquid which contaminate the environment. Experiments to evaluate the effect of natural soil ameliorant derived from coffee pulp and minerals were conducted at The Indonesian Coffee and Cocoa Research Institute in Jember, East Java. The experiments were intended to optimize the use of coffee pulp to support farming sustainability and minimize negative impacts of solid waste disposal originated from coffee cherry processing. Prior to applications, coffee pulp was hulled to organic paste. The paste was then mixed with 10% minerals (b/b. Composition of the minerals was 50% zeolite and 50% rock phosphate powder. The ameliorant was characterized for their physical and chemical properties. Agronomic tests were conducted on coffee and cocoa seedling. The experiments were arranged according to Randomized Completely Design with 2 factors, consisted of natural ameliorant and inorganic fertilizer respectively. Natural ameliorant derived from coffee pulp was applied at 6 levels: 0, 30, 60, 90, 120 and 150 g dry ameliorant/seedling of 3 kg soil, equivalent to 0, 1, 2, 3, 4 and 5% (b/b of ameliorant respectively. Inorganic fertilizer was applied at 2 levels: 0 and 2 g fertilizer/application of N-P-K compound fertilizer of 15-15-15 respectively. The inorganic fertilizer was applied 4 times during nursery of coffee and cocoa. The result of the experiment indicated that coffee pulp may be used as natural soil ameliorant. Composition of ameliorant of 90% coffee pulp and 10% of minerals has good physical and chemical characteristics for soil amelioration. The composition has high water holding capacity; cations exchange capacity, organic carbon and phosphorus contents which are favorable to increase soil capacity to support plant growth. Application of

  3. Secretory Vesicle Priming by CAPS Is Independent of Its SNARE-Binding MUN Domain

    Directory of Open Access Journals (Sweden)

    Cuc Quynh Nguyen Truong

    2014-11-01

    Full Text Available Priming of secretory vesicles is a prerequisite for their Ca2+-dependent fusion with the plasma membrane. The key vesicle priming proteins, Munc13s and CAPSs, are thought to mediate vesicle priming by regulating the conformation of the t-SNARE syntaxin, thereby facilitating SNARE complex assembly. Munc13s execute their priming function through their MUN domain. Given that the MUN domain of Ca2+-dependent activator protein for secretion (CAPS also binds syntaxin, it was assumed that CAPSs prime vesicles through the same mechanism as Munc13s. We studied naturally occurring splice variants of CAPS2 in CAPS1/CAPS2-deficient cells and found that CAPS2 primes vesicles independently of its MUN domain. Instead, the pleckstrin homology domain of CAPS2 seemingly is essential for its priming function. Our findings indicate a priming mode for secretory vesicles. This process apparently requires membrane phospholipids, does not involve the binding or direct conformational regulation of syntaxin by MUN domains of CAPSs, and is therefore not redundant with Munc13 action.

  4. Dentin and pulp sense cold stimulus.

    Science.gov (United States)

    Tokuda, Masayuki; Tatsuyama, Shoko; Fujisawa, Mari; Morimoto-Yamashita, Yoko; Kawakami, Yoshiko; Shibukawa, Yoshiyuki; Torii, Mistuso

    2015-05-01

    Dentin hypersensitivity is a common symptom, and recent convergent evidences have reported transient receptor potential (TRP) channels in odontoblasts act as mechanical and thermal molecular sensor, which detect stimulation applied on the exposed dentin surface, to drive multiple odontoblastic cellular functions, such as sensory transduction and/or dentin formation. In the present study, we confirmed expression of TRP melastatin subfamily member-8 (TRPM8) channels in primary cultured cells derived from human dental pulp cells (HPCs) and mouse odontoblast-lineage cells (OLCs) as well as in dentin matrix protein-1 (DMP-1) and dentin sialoprotein (DSP) positive acutely isolated rat odontoblasts from dental pulp tissue slice culture by immunohistochemical analyses. In addition, we detected TRPM8 channel expression on HPCs and OLCs by RT-PCR and Western blotting analyses. These results indicated that both odontoblasts and dental pulp cells express TRPM8 channels in rat, mouse and human, and therefore we hypothesize they may contribute as cold sensor in tooth. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Hard tissue deposition in dental pulp canal by {alpha}-tricalcium phosphate cement

    Energy Technology Data Exchange (ETDEWEB)

    Yoshikawa, M.; Toda, T. [Osaka Dental Univ. (Japan). Dept. of Endodontics; Mandai, Y. [Bio-Chemical Lab. of Nitta Gelatin Inc., Yao (Japan); Oonishi, H. [Osaka Minami National Hospital, Kawachi (Japan). Dept. of Orthopaedic Surgery

    2001-07-01

    Canal closure by hard tissue proliferation in the pulp canal and/or apical foramen is the most ideal healing after pulp removal. Generally, Ca(OH){sub 2} may induce secondary dentine or dentine-bridge on the amputated pulp surface. However, Ca(OH){sub 2} shows strong alkalinity and may cause severe inflammatory responses in the residual pulp. Moreover, completely formed dentine-bridge at the orifice will disturb further treatment of residual pulp because of the difficulty in localizing the pathway. The purpose of this study was to see hard tissue induction using newly developed {alpha}-tricalcium phosphate cement and to recognize the morphological difference of hard tissue from that of Ca(OH){sub 2}. (orig.)

  6. The role of bound chlorine in the brightness reversion of bleached hardwood kraft pulp

    Directory of Open Access Journals (Sweden)

    Kátia Maria Morais Eiras

    2009-01-01

    Full Text Available Our previous paper showed fragmentary evidence that pulp brightness reversion may be negatively affected by its organically bound chlorine (OX content. A thorough investigation on eucalyptus kraft pulp led to the conclusion that OX increases reversion of certain pulps but this trend is not universal. Alkaline bleaching stages decrease reversion regardless of pulp OX content. Pulps bleached with high temperature chlorine dioxide revert less than those bleached with conventional chlorine dioxide in sequences ending with a chlorine dioxide stage but similarly in sequences ending with a final peroxide stage. The use of secondary condensate for pulp washing decreases reversion.

  7. Competitiveness of wood pulp production in different Brazilian states

    Directory of Open Access Journals (Sweden)

    Naisy Silva Soares

    2013-06-01

    Full Text Available This work aimed to analyze the competitiveness of wood pulp production in different Brazilian states, in May, 2008 (Minas Gerais, São Paulo, Espírito Santo and Bahia, using the Policy Analysis Matrix (PAM. The results obtained indicated that the private and social profitability of wood pulp production and commercialization was positive and greater in Bahia. The Brazilian companies were penalized by public policies adopted for the sector; the wood pulp production in São Paulo and Bahia were more competitive and less exposed to the negative effects of public policies that reduce the national company profits.

  8. MOLYBDENUM CATALYZED ACID PEROXIDE BLEACHING OF EUCALYPTUS KRAFT PULP

    Directory of Open Access Journals (Sweden)

    Marcos S. Rabelo

    2008-08-01

    Full Text Available Molybdenum catalyzed peroxide bleaching (PMo Stage consists of pulp treatment with hydrogen peroxide under acidic conditions in the presence of a molybdenum catalyst. Molybdenum is applied in catalytic doses (50-200 mg/kg pulp and may originate from various sources, including (NH46Mo7O24.4H2O, Na2MoO4.2H2O, siliconmolybdate, etc. This work is aimed at optimizing the PMo stage and evaluating its industrial application in the OAZDP sequence. Optimum PMo stage conditions for bleaching eucalyptus pulp were 90 ºC, pH 3.5, 2 h, 0.1 kg/adt Mo and 5 kg/adt H2O2. The PMo stage was more efficient to remove pulp hexenuronic acids than lignin. Its efficiency decreased with increasing pH in the range of 1.5-5.5, while it increased with increasing temperature and peroxide and molybdenum doses. The application of the PMo stage as replacement for the A-stage of the AZDP sequence significantly decreased chlorine dioxide demand. The PMo stage caused a decrease of 20-30% in the generation of organically bound chlorine. The quality parameters of the pulp produced during the PMo stage mill trial were comparable to those obtained with the reference A-stage.

  9. Microtubule's conformational cap

    DEFF Research Database (Denmark)

    Flyvbjerg, H.

    1999-01-01

    The molecular mechanisms that allow elongation of the unstable microtubule lattice remain unclear. It is usually thought that the GDP-liganded tubulin lattice is capped by a small layer of GTP- or GDP-P(i)-liganded molecules, the so called "GTP-cap". Here, we point-out that the elastic properties...

  10. Preparation of lumen-loaded kenaf pulp with magnetite (Fe3O4)

    International Nuclear Information System (INIS)

    Zakaria, S.; Ong, B.H.; Ahmad, S.H.; Abdullah, M.; Yamauchi, T.

    2005-01-01

    Magnetic pulps were prepared from unbleached kenaf (hibiscus cannabinus L.) kraft pulps. Fe 3 O 4 or magnetite powder was used to load into the pulp's lumen and pit. Aluminum sulphate [Al 2 (SO 4 ) 3 ] (alum) and polyethylenimine (PEI), both mainly function as retention aid were used throughout the experiment and found to be beneficial in the preparation of this magnetic pulps. The ash content method was used to determine the amount of magnetite retained in the lumen and pit. The utilization of PEI up to 2% per pulp fibres was found to be the best result on lumen loading. The deposition of magnetite powder in lumen and pit is found decrease as the addition of PEI used is more than 2% per pulp fibres. Scanning electron microscope (SEM) clearly shows the distribution of magnetite deposited in the lumen. Tensile index and folding endurance of the loaded fibre decreased slightly as the percentage of loading pigment increased

  11. Pulp Obliteration in a Patient with Sclerodermatous Chronic Graft-versus-Host Disease.

    Science.gov (United States)

    Gomes, Camilla Borges Ferreira; Treister, Nathaniel Simon; Miller, Brian; Armand, Philippe; Friedland, Bernard

    2016-04-01

    Dental pulp calcification is a common finding associated with localized dental trauma, genetic disorders, and systemic inflammatory diseases. Chronic graft-versus-host disease (cGVHD) is a frequent complication after allogeneic hematopoietic cell transplantation (allo-HCT) characterized by immune-mediated injury to the skin, mouth, eyes, liver, and other tissues, resulting in significant disability and reduced quality of life. We report a patient with sclerodermatous cGVHD who presented with general pulp calcification in all teeth 5 years after allo-HCT. A review of full mouth dental radiographs obtained just before allo-HCT revealed normal-appearing pulp chambers. Based on prior reports of generalized pulp calcification associated with progressive systemic sclerosis, we hypothesized that the etiology was likely related to the presence of cGVHD with associated vascular and fibrotic tissue changes within the pulp vasculature. Clinicians should consider cGVHD in the differential diagnosis of generalized pulp calcification. Copyright © 2016 American Association of Endodontists. All rights reserved.

  12. Yeasts associated with fresh and frozen pulps of Brazilian tropical fruits.

    Science.gov (United States)

    Trindade, Rita C; Resende, Maria Aparecida; Silva, Claudia M; Rosa, Carlos A

    2002-08-01

    The occurrence of yeasts on ripe fruits and frozen pulps of pitanga (Eugenia uniflora L), mangaba (Hancornia speciosa Gom.), umbu (Spondias tuberosa Avr. Cam.), and acerola (Malpighia glaba L) was verified. The incidence of proteolytic, pectinolytic, and mycocinogenic yeasts on these communities was also determined. A total of 480 colonies was isolated and grouped in 405 different strains. These corresponded to 42 ascomycetous and 28 basidiomycetous species. Candida sorbosivorans, Pseudozyma antarctica, C. spandovensis-like, C. spandovensis, Kloeckera apis, C. parapsilosis, Rhodotorula graminis, Kluyveromyces marxianus, Cryptococcus laurentii, Metchnikowia sp (isolated only from pitanga ripe fruits), Issatchenkia occidentalis and C. krusei (isolated only from mangaba frozen pulps), were the most frequent species. The yeast communities from pitanga ripe fruits exhibited the highest frequency of species, followed by communities from acerola ripe fruits and mangaba frozen pulps. Yeast communities from frozen pulp and ripe fruits of umbu had the lowest number of species. Except the yeasts from pitanga, yeast communities from frozen pulp exhibited higher number of yeasts than ripe fruit communities. Mycocinogenic yeasts were found in all of the substrates studied except in communities from umbu ripe fruits and pitanga frozen pulps. Most of the yeasts found to produce mycocins were basidiomycetes and included P. antarctica, Cryptococcus albidus, C. bhutanensis-like, R. graminis and R. mucilaginosa-like from pitanga ripe fruits as well as black yeasts from pitanga and acerola ripe fruits. The umbu frozen pulps community had the highest frequency of proteolytic species. Yeasts able to hydrolyse casein at pH 5.0 represented 38.5% of the species isolated. Thirty-seven percent of yeast isolates were able to hydrolyse casein at pH 7.0. Pectinolytic yeasts were found in all of the communities studied, excepted for those of umbu frozen pulps. The highest frequency of

  13. Strategies for Industry Internationalization Brazilian Pulp and Paper the Perspective of Eclectic Paradigm: A Case Study of Pulp and Paper Company Suzano

    Directory of Open Access Journals (Sweden)

    Mayra Batista Bitencourt Fagundes

    2012-12-01

    Full Text Available This article aims to analyze the process of internationalization of the Brazilian pulp and paper. This analysis has an economic bias, basing on the model of internationalization of Eclectic Paradigm of Dunning (1980 in order to identify the internationalization strategies, as well as the key drivers that have enabled reach new markets. We adopted a qualitative methodology, developed through a case study in the company Suzano, a Brazilian company, a global leader in the pulp and paper industry, with a strong presence in the international market. The data were obtained from literature searches, statistics, press releases and official documents of the company. For the treatment of these was used to pattern matching techniques, which enabled contrast them with the theory selected. The results revealed that Suzano differentiated adopted guidelines for the marketing of paper and pulp. The paper is intended primarily for the domestic market, whereas most of the pulp produced is intended to international trade. The company went international for having, according to the Eclectic Paradigm, the advantages of ownership and internalization over their competitors. The strategies used consists in finding markets (market seeking, search efficiency (efficiency seeking and search for strategic assets (strategic asset seeking. We conclude that the theory is Eclectic Paradigm in an appropriate tool for analyzing the internationalization process of the pulp and paper industries.

  14. Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells

    Directory of Open Access Journals (Sweden)

    Choo-Ryung Chung

    2012-02-01

    Full Text Available Objectives The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day of differentiation.

  15. 75 FR 49527 - Caps Visual Communications, LLC; Black Dot Group; Formerly Known as Caps Group Acquisition, LLC...

    Science.gov (United States)

    2010-08-13

    ... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-74,195] Caps Visual Communications, LLC; Black Dot Group; Formerly Known as Caps Group Acquisition, LLC Chicago, IL; Amended... of Caps Visual Communications, LLC, Black Dot Group, formerly known as Caps Group Acquisition, LLC...

  16. Can dental pulp calcification predict the risk of ischemic cardiovascular disease?

    Science.gov (United States)

    Khojastepour, Leila; Bronoosh, Pegah; Khosropanah, Shahdad; Rahimi, Elham

    2013-09-01

    To report the association of pulp calcification with that of cardiovascular disease (CVD) using digital panoramic dental radiographs. Digital panoramic radiographs of patients referred from the angiography department were included if the patient was under 55 years old and had non-restored or minimally restored molars and canines. An oral and maxillofacial radiologist evaluated the images for pulpal calcifications in the selected teeth. The sensitivity, specificity, positive predictive value and negative predictive value of panoramic radiography in predicting CVD were calculated. Out of 122 patients who met the criteria, 68.2% of the patients with CVD had pulp chamber calcifications. Pulp calcification in panoramic radiography had a sensitivity of 68.9% to predict CVD. This study demonstrates that patients with CVD show an increased incidence of pulp calcification compared with healthy patients. The findings suggest that pulp calcification on panoramic radiography may have possibilities for use in CVD screening.

  17. Mechanisms underlying ectopic persistent tooth-pulp pain following pulpal inflammation.

    Directory of Open Access Journals (Sweden)

    Shingo Matsuura

    Full Text Available In order to clarify the peripheral mechanisms of ectopic persistent pain in a tooth pulp following pulpal inflammation of an adjacent tooth, masseter muscle activity, phosphorylated extracellular signal-regulated protein kinase (pERK and TRPV1 immunohistochemistries and satellite cell activation using glial fibrillary acidic protein (GFAP immunohistochemistry in the trigeminal ganglion (TG were studied in the rats with molar tooth-pulp inflammation. And, Fluorogold (FG and DiI were also used in a neuronal tracing study to analyze if some TG neurons innervate more than one tooth pulp. Complete Freund's adjuvant (CFA or saline was applied into the upper first molar tooth pulp (M1 in pentobarbital-anesthetized rats, and capsaicin was applied into the upper second molar tooth pulp (M2 on day 3 after the CFA or saline application. Mean EMG activity elicited in the masseter muscle by capsaicin application to M2 was significantly larger in M1 CFA-applied rats compared with M1 vehicle-applied rats. The mean number of pERK-immunoreactive (IR TG cells was significantly larger in M1 CFA-applied rats compared with M1 vehicle-applied rats. Application of the satellite cell inhibitor fluorocitrate (FC into TG caused a significant depression of capsaicin-induced masseter muscle activity and a significant reduction of satellite cell activation. The number of TRPV1-IR TG cells innervating M2 was significantly larger in M1 CFA-applied rats compared with M1 vehicle-applied rats, and that was decreased following FC injection into TG. Furthermore, 6% of TG neurons innervating M1 and/or M2 innervated both M1 and M2. These findings suggest that satellite cell activation following tooth pulp inflammation and innervation of multiple tooth pulps by single TG neurons may be involved in the enhancement of the activity of TG neurons innervating adjacent non-inflamed teeth that also show enhancement of TRPV1 expression in TG neurons, resulting in the ectopic persistent tooth-pulp

  18. Distinctive genetic activity pattern of the human dental pulp between deciduous and permanent teeth.

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    Ji-Hee Kim

    Full Text Available Human deciduous and permanent teeth exhibit different developmental processes, morphologies, histological characteristics and life cycles. In addition, their pulp tissues react differently to external stimuli, such as the pulp sensitivity test, dental trauma and pulp therapy materials. These suggest differences in gene expression and regulation, and in this study we compared gene-expression profiles of the human dental pulp from deciduous and permanent teeth. Pulp tissues from permanent premolars and deciduous molars aged 11-14 years were extirpated and mRNA was isolated for cDNA microarray analysis, and quantitative real-time PCR (qPCR. Other teeth were used for immunohistochemical analysis (IHC. Microarray analysis identified 263 genes with a twofold or greater difference in expression level between the two types of pulp tissue, 43 and 220 of which were more abundant in deciduous and permanent pulp tissues, respectively. qPCR analysis was conducted for eight randomly selected genes, and the findings were consistent with the cDNA microarray results. IHC confirmed that insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1 was broadly expressed in deciduous dental pulp tissue, but minimally expressed in permanent dental pulp tissue. Immunohistochemical analysis showed that calbindin 1 (CALB1, leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5, and gamma-aminobutyric acid A receptor beta 1 (GABRB1 were abundantly expressed in permanent predentin/odontoblasts, but only minimally expressed in deciduous dental pulp tissue. These results show that deciduous and permanent pulp tissues have different characteristics and gene expression, suggesting that they may have different functions and responses to therapies focused on pulp or dentin regeneration.

  19. Distinctive genetic activity pattern of the human dental pulp between deciduous and permanent teeth.

    Science.gov (United States)

    Kim, Ji-Hee; Jeon, Mijeong; Song, Je-Seon; Lee, Jae-Ho; Choi, Byung-Jai; Jung, Han-Sung; Moon, Seok Jun; DenBesten, Pamela K; Kim, Seong-Oh

    2014-01-01

    Human deciduous and permanent teeth exhibit different developmental processes, morphologies, histological characteristics and life cycles. In addition, their pulp tissues react differently to external stimuli, such as the pulp sensitivity test, dental trauma and pulp therapy materials. These suggest differences in gene expression and regulation, and in this study we compared gene-expression profiles of the human dental pulp from deciduous and permanent teeth. Pulp tissues from permanent premolars and deciduous molars aged 11-14 years were extirpated and mRNA was isolated for cDNA microarray analysis, and quantitative real-time PCR (qPCR). Other teeth were used for immunohistochemical analysis (IHC). Microarray analysis identified 263 genes with a twofold or greater difference in expression level between the two types of pulp tissue, 43 and 220 of which were more abundant in deciduous and permanent pulp tissues, respectively. qPCR analysis was conducted for eight randomly selected genes, and the findings were consistent with the cDNA microarray results. IHC confirmed that insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) was broadly expressed in deciduous dental pulp tissue, but minimally expressed in permanent dental pulp tissue. Immunohistochemical analysis showed that calbindin 1 (CALB1), leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), and gamma-aminobutyric acid A receptor beta 1 (GABRB1) were abundantly expressed in permanent predentin/odontoblasts, but only minimally expressed in deciduous dental pulp tissue. These results show that deciduous and permanent pulp tissues have different characteristics and gene expression, suggesting that they may have different functions and responses to therapies focused on pulp or dentin regeneration.

  20. Obtaining value prior to pulping with diethyl oxalate and oxalic acid

    Science.gov (United States)

    W.R. Kenealy; E. Horn; C.J. Houtman; J. Laplaza; T.W. Jeffries

    2007-01-01

    Pulp and paper are converted to paper products with yields of paper dependent on the wood and the process used. Even with high yield pulps there are conversion losses and with chemical pulps the yields approach 50%. The portions of the wood that do not provide product are either combusted to generate power and steam or incur a cost in waste water treatment. Value prior...

  1. Macrophage Capping Protein CapG Is a Putative Oncogene Involved in Migration and Invasiveness in Ovarian Carcinoma

    Directory of Open Access Journals (Sweden)

    J. Glaser

    2014-01-01

    Full Text Available The actin binding protein CapG modulates cell motility by interacting with the cytoskeleton. CapG is associated with tumor progression in different nongynecologic tumor entities and overexpression in breast cancer cell lines correlates with a more invasive phenotype in vitro. Here, we report a significant CapG overexpression in 18/47 (38% of ovarian carcinomas (OC analyzed by qRealTime-PCR analyses. Functional analyses in OC cell lines through siRNA mediated CapG knockdown and CapG overexpression showed CapG-dependent cell migration and invasiveness. A single nucleotide polymorphism rs6886 inside the CapG gene was identified, affecting a CapG phosphorylation site and thus potentially modifying CapG function. The minor allele frequency (MAF of SNP rs6886 (c.1004A/G was higher and the homozygous (A/A, His335 genotype was significantly more prevalent in patients with fallopian tube carcinomas (50% as in controls (10%. With OC being one of the most lethal cancer diseases, the detection of novel biomarkers such as CapG could reveal new diagnostic and therapeutic targets. Moreover, in-depth analyses of SNP rs6886 related to FTC and OC will contribute to a better understanding of carcinogenesis and progression of OC.

  2. Proliferation and mineralization ability of dental pulp cells derived from primary and permanent teeth

    Directory of Open Access Journals (Sweden)

    Suttatip Kamolmatyakul

    2011-04-01

    Full Text Available The aims of this study were to compare the proliferation and mineralization ability of CFU-F selected dental pulp cellsderived from primary and permanent teeth. Those cells were isolated by enzyme digestion and analyzed for their colonyformingcapacity. The cell proliferation was measured by the MTT assay on day 1, day 7, and day14. Alizarin Red S stainingwas used to detect mineralized nodule formation of the cells on day 7, 14, 21, and 28. Proliferation of CFU-F selected pulpcells from primary teeth was significantly higher than that of CFU-F selected pulp cells from permanent teeth in all periods ofthe experiment. Upon cultured cells in mineralization inducing media, the mineralized nodules appeared as early as day 14 inCFU-F selected pulp cells from primary teeth and MG-63, whereas those of CFU-F selected pulp cells from permanent teethcan be found at day 21. On day 21 and day 28, the mineralized nodules of the CFU-F selected pulp cells from the primaryteeth group were more than those in the CFU-F selected pulp cells from the permanent teeth group. Mineralized noduleformation in the CFU-F selected pulp cells from the permanent teeth group appeared later and were less than those ofCFU-F selected pulp cells from primary teeth. However, mineralized nodules in CFU-F selected pulp cells from the permanentteeth group increased very fast after their appearance. Those results suggest that CFU-F selected pulp cells from primaryteeth had a higher proliferation rate and mineralization rate when compared to CFU-F selected pulp cells from permanentteeth.

  3. HYDROGEN PEROXIDE BLEACHING OF CMP PULP USING MAGNESIUM HYDROXIDE

    Directory of Open Access Journals (Sweden)

    Farhad Zeinaly

    2009-11-01

    Full Text Available Conventional bleaching of hardwood CMP pulp with magnesium hydroxide (Mg(OH2 show significant benefits over bleaching with sodium hydroxide (NaOH under various conditions. Magnesium hydroxide bleaching generate higher optical properties, higher pulp yield and lower effluent COD at the same chemical charge, but the physical properties were found to be similar for both processes. The initial freeness of the bleached pulps and refining value to reach a target freeness (about 350 ml. CSF were more for the Mg(OH2-based process. The residual peroxide of filtrate from the Mg(OH2-based process was very high as compared to conventional bleaching.

  4. Optimization of Dithionite Bleaching of High Yield Bagasse Pulp

    International Nuclear Information System (INIS)

    Mohamed, E.

    2005-01-01

    High yield bagasse pulp was prepared by soaking bagasse in 10 % cold sodium hydroxide for 24 hours and then refluxing for two hours at 95 degree C. Optimization of dithionite bleaching was attained by investigation of different parameters as consistency, temperature, time and ph. Effect of additives as chelating agent (EDTA) or stabilizing of bleaching solution (Zn compound and hexamethylene tetramine) was considered. The effect of air content was also studied. One and two stages bleaching of the pulp were investigated by using sodium dithionite (D) as a sole bleaching agent or after application of one stage bleaching by hydrogen peroxide to attain high brightness for high yield pulp

  5. Universal industrial sectors integrated solutions module for the pulp and paper industry.

    Science.gov (United States)

    Bhander, Gurbakhash; Jozewicz, Wojciech

    2017-09-01

    The U.S. is the world's second-leading producer of pulp and paper products after China. Boilers, recovery furnaces, and lime kilns are the dominant sources of emissions from pulp and paper mills, collectively accounting for more than 99 % of the SO 2 , almost 96 % of the NO X , and more than 85 % of the particulate matter (PM) emitted to the air from this sector in the U.S. The process of developing industrial strategies for managing emissions can be made efficient, and the resulting strategies more cost-effective, through the application of modeling that accounts for relevant technical, environmental and economic factors. Accordingly, the United States Environmental Protection Agency is developing the Universal Industrial Sectors Integrated Solutions module for the Pulp and Paper Industry (UISIS-PNP). It can be applied to evaluate emissions and economic performance of pulp and paper mills separately under user-defined pollution control strategies. In this paper, we discuss the UISIS-PNP module, the pulp and paper market and associated air emissions from the pulp and paper sector. After illustrating the sector-based multi-product modeling structure, a hypothetical example is presented to show the engineering and economic considerations involved in the emission-reduction modeling of the pulp and paper sector in the U.S.

  6. Can Dental Pulp Calcification Predict the Risk of Ischemic Cardiovascular Disease?

    Directory of Open Access Journals (Sweden)

    Leila Khojastepour

    2013-01-01

    Full Text Available Objective: To report the association of pulp calcification with that of cardiovascular disease (CVD using digital panoramic dental radiographs.Materials and Methods: Digital panoramic radiographs of patients referred from the angiography department were included if the patient was under 55 years old and had non-restored or minimally restored molars and canines. An oral and maxillofacial radiologist evaluated the images for pulpal calcifications in the selected teeth. The sensitivity, specificity, positive predictive value and negative predictive value of panoramic radiography in predicting CVD were calculated.Results: Out of 122 patients who met the criteria, 68.2% of the patients with CVD had pulp chamber calcifications. Pulp calcification in panoramic radiography had a sensitivity of 68.9% to predict CVD.Conclusion: This study demonstrates that patients with CVD show an increased incidence of pulp calcification compared with healthy patients. The findings suggest that pulp calcification on panoramic radiography may have possibilities for use in CVD screening.

  7. Application of thermophilic enzymes and water jet system to cassava pulp.

    Science.gov (United States)

    Chaikaew, Siriporn; Maeno, Yuka; Visessanguan, Wonnop; Ogura, Kota; Sugino, Gaku; Lee, Seung-Hwan; Ishikawa, Kazuhiko

    2012-12-01

    Co-production of fermentable sugars and nanofibrillated cellulose from cassava pulp was achieved by the combination of thermophilic enzymes (endoglucanase, β-glucosidase, and α-amylase) and a new atomization system (Star Burst System; SBS), which employs opposing water jets. The SBS represents a key technology for providing cellulose nanofibers and improving the enzymatic saccharification of cassava pulp. Depending on the enzymes used, the production of glucose from cassava pulp treated with the SBS was 1.2- to 2.5-fold higher than that from pulp not treated with the SBS. Nanofibrillated cellulose with the gel-like property in suspension was produced (yield was over 90%) by α-amylase treatment, which completely released trapped starch granules from the fibrous cell wall structure of cassava pulp pretreated with the SBS. The SBS provides an environmentally low-impact pretreatment system for processing biomass material into value-added products. Copyright © 2012 Elsevier Ltd. All rights reserved.

  8. Enzymatic Extraction of Hydroxycinnamic Acids from Coffee Pulp

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    Ernesto Favela-Torres

    2011-01-01

    Full Text Available Ferulic, caffeic, p-coumaric and chlorogenic acids are classified as hydroxycinnamic acids, presenting anticarcinogenic, anti-inflammatory and antioxidant properties. In this work, enzymatic extraction has been studied in order to extract high value-added products like hydroxycinnamic acids from coffee pulp. A commercial pectinase and enzyme extract produced by Rhizomucor pusillus strain 23aIV in solid-state fermentation using olive oil or coffee pulp (CP as an inducer of the feruloyl esterase activity were evaluated separately and mixed. The total content (covalently linked and free of ferulic, caffeic, p-coumaric and chlorogenic acids was 5276 mg per kg of coffee pulp. Distribution was as follows (in %: chlorogenic acid 58.7, caffeic acid 37.6, ferulic acid 2.1 and p-coumaric acid 1.5. Most of the hydroxycinnamic acids were covalently bound to the cell wall (in %: p-coumaric acid 97.2, caffeic acid 94.4, chlorogenic acid 76.9 and ferulic acid 73.4. The content of covalently linked hydroxycinnamic acid was used to calculate the enzyme extraction yield. The maximum carbon dioxide rate for the solid-state fermentation using olive oil as an inducer was higher and it was reached in a short cultivation time. Nevertheless, the feruloyl esterase (FAE activity (units per mg of protein obtained in the fermentation using CP as an inducer was 31.8 % higher in comparison with that obtained in the fermentation using olive oil as the inducer. To our knowledge, this is the first report indicating the composition of both esterified and free ferulic, caffeic, p-coumaric and chlorogenic acids in coffee pulp. The highest yield of extraction of hydroxycinnamic acids was obtained by mixing the produced enzyme extract using coffee pulp as an inducer and a commercial pectinase. Extraction yields were as follows (in %: chlorogenic acid 54.4, ferulic acid 19.8, p-coumaric acid 7.2 and caffeic acid 2.3. An important increase in the added value of coffee pulp was mainly

  9. Eddy intrusion of hot plasma into the polar cap and formation of polar-cap arcs

    International Nuclear Information System (INIS)

    Chiu, Y.T.; Gorney, D.J.

    1983-01-01

    We present plasma and electric field data obtained by the S3-3 satellite over the polar caps. We demonstrate that: (1) plasma signatures in the polar cap arc formation region near 5000 km altitude show clear intrusions of plasma sheet (approx.keV) and magneto sheath (approx.100 eV) plasma into a background of low-energy polar cap plasma; (2) the combined plasma and electric field signatures (electron inverted-V, ion beam and delxE<0) are exactly the same as in the evening discrete arc. We interpret this equivalence of polar cap and evening discrete arc signatures as indication that their formation processes are identical. The spatial structures of polar cap electric fields and the associated plasma signatures are consistent with the hypothesis that plasma intrusion into the polar cap takes the form of multiple cellular eddies. This hypothesis provides a unifying view of arc formation and arc configurations

  10. Innervated boomerang flap for finger pulp reconstruction.

    Science.gov (United States)

    Chen, Shao-Liang; Chiou, Tai-Fung

    2007-11-01

    The boomerang flap originates from the dorsolateral aspect of the proximal phalanx of an adjacent digit and is supplied by the retrograde blood flow through the vascular arcades between the dorsal and palmar digital arteries. To provide sensation of the boomerang flap for finger pulp reconstruction, the dorsal sensory branch of the proper digital nerve and the superficial sensory branch of the corresponding radial or ulnar nerve are included within the skin flap. After transfer of the flap to the injured site, epineural neurorrhaphies are done between the digital nerves of the pulp and the sensory branches of the flap. We used this sensory flap in five patients, with more than 1 year follow-up, and all patients achieved measurable two-points discrimination. The boomerang flap not only preserves the proper palmar digital artery but also provides an extended and innervated skin paddle. It seems to be an alternative choice for one-stage reconstruction of major pulp defect.

  11. Characterization of active paper packaging incorporated with ginger pulp oleoresin

    Science.gov (United States)

    Wiastuti, T.; Khasanah, L. U.; Atmaka Kawiji, W.; Manuhara, G. J.; Utami, R.

    2016-02-01

    Utilization of ginger pulp waste from herbal medicine and instant drinks industry in Indonesia currently used for fertilizer and fuel, whereas the ginger pulp still contains high oleoresin. Active paper packaging were developed incorporated with ginger pulp oleoresin (0%, 2%, 4%, and 6% w/w). Physical (thickness, tensile strength, and folding endurance, moisture content), sensory characteristics and antimicrobial activity of the active paper were evaluated. Selected active paper then were chemically characterized (functional groups). The additional of ginger pulp oleoresin levels are reduced tensile strength, folding endurance and sensory characteristic (color, texture and overall) and increased antimicrobial activity. Due to physical, sensory characteristic and antimicrobial activity, active paper with 2% ginger pulp oleoresin incorporation was selected. Characteristics of selected paper were 9.93% of water content; 0.81 mm of thickness; 0.54 N / mm of tensile strength; 0.30 of folding endurance; 8.43 mm inhibits the growth of Pseudomonas fluorescence and 27.86 mm inhibits the growth of Aspergillus niger (antimicrobial activity) and neutral preference response for sensory properties. For chemical characteristic, selected paper had OH functional group of ginger in 3422.83 cm-1 of wave number and indicated contain red ginger active compounds.

  12. Dentin and dental pulp regeneration by the patient's endogenous cells.

    Science.gov (United States)

    Kim, Sahng G; Zheng, Ying; Zhou, Jian; Chen, Mo; Embree, Mildred C; Song, Karen; Jiang, Nan; Mao, Jeremy J

    2013-03-01

    The goal of regenerative endodontics is to restore the functions of the dental pulp-dentin complex. Two approaches are being applied toward dental pulp-dentin regeneration: cell transplantation and cell homing. The majority of previous approaches are based on cell transplantation by delivering ex vivo cultivated cells toward dental pulp or dentin regeneration. Many hurdles limit the clinical translation of cell transplantation such as the difficulty of acquiring and isolating viable cells, uncertainty of what cells or what fractions of cells to use, excessive cost of cell manipulation and transportation, and the risk of immune rejection, pathogen transmission, and tumorigenesis in associated with ex vivo cell manipulation. In contrast, cell homing relies on induced chemotaxis of endogenous cells and therefore circumvents many of the difficulties that are associated with cell transplantation. An array of proteins, peptides, and chemical compounds that are yet to be identified may orchestrate endogenous cells to regenerate dental pulp-dentin complex. Both cell transplantation and cell homing are scientifically valid approaches; however, cell homing offers a number of advantages that are compatible with the development of clinical therapies for dental pulp-dentin regeneration.

  13. Designing Smart Charter School Caps

    Science.gov (United States)

    Dillon, Erin

    2010-01-01

    In 2007, Andrew J. Rotherham proposed a new approach to the contentious issue of charter school caps, the statutory limits on charter school growth in place in several states. Rotherham's proposal, termed "smart charter school caps," called for quality sensitive caps that allow the expansion of high-performing charter schools while also…

  14. Xylanase-Aided Chlorine Dioxide Bleaching of Bagasse Pulp to Reduce AOX Formation

    Directory of Open Access Journals (Sweden)

    Yi Dai

    2016-02-01

    Full Text Available Xylanase pretreatment was used to improve the chlorine dioxide bleaching of bagasse pulp. The pulp was pretreated with xylanase, which was followed by a chlorine dioxide bleaching stage. The HexA content of the pulp and the AOX content of the bleaching effluent were measured using UV-Vis and GC-MS methods, respectively. The results showed that a good correlation occurred between HexA and kappa number. HexA content of the pulp decreased significantly after the xylanase pretreatment. The AOX content of the bleaching effluent decreased as HexA was removed from the pulp. It was found that AOX could be reduced by up to 29.8%, comparing XD0 with a D0 stage. Fourier transform infrared spectroscopy (FTIR was employed to determine the breakage of chemical bonds in the pulp. It revealed that some lignin and hemicellulose were removed after xylanase treatment. The GC-MS results showed that some toxic chloride such as 2,4,6-trichlorophenol could be completely removed after xylanase pretreatment.

  15. Laboratory testing of closure cap repair techniques

    International Nuclear Information System (INIS)

    Persoff, P.; Moridis, G.; Tuck, D.M.

    1996-10-01

    Landfill design requires a low permeability closure cap as well as a low permeability liner. The Savannah River Site, in South Carolina, has approximately 85 acres of mixed waste landfills covered with compacted kaolin clay. Maintaining low permeability of the clay cap requires both that the permeability of the compacted clay itself remain low and that the integrity of the barrier be maintained. Barrier breaches typically result from penetration by roots or animals, and especially cracks caused by uneven settling or desiccation. In this study, clay layers, 0.81 m in diameter and 7.6 cm thick, were compacted in 7 lysimeters to simulate closure caps. The hydraulic conductivity of each layer was measured, and the compacted clay layers (CCL's) were cracked by drying. Then various repair techniques were applied and the effectiveness of each repair was assessed by remeasuring the hydraulic conductivity. Finally the repaired CCL was again dried and measured to determine how the repair responded to the conditions that caused the original failure. For a full report of this investigation see Persoff et al. Six repair techniques have been tested, four of which involve the use of injectable barrier liquids colloidal silica (CS) and polysiloxane (PSX) described below: (I) covering the crack with a bentonite geosynthetic clay liner (GCL), (ii) recompaction of new kaolinite at STD+3 moisture content joined to existing kaolinite that had dried and shrunk, (iii) direct injection of colloidal silica to a crack, (iv) injection of colloidal silica (CS) to wells in an overlying sand layer, (v) direct injection of polysiloxane to a crack, and (vi), injection of polysiloxane (PSX) to wells in an overlying soil layer

  16. Pulp Calcification in Traumatized Primary Teeth - Classification, Clinical And Radiographic Aspects.

    Science.gov (United States)

    Mello-Moura, Anna Carolina Volpi; Santos, Ana Maria Antunes; Bonini, Gabriela Azevedo Vasconcelos Cunha; Zardetto, Cristina Giovannetti Del Conte; Moura-Netto, Cacio; Wanderley, Marcia Turolla

    The aim of this study was to standardize the nomenclature of pulp alteration to pulp calcification (PC) and to classify it according to type, quantity and location, as well as relate it to clinical and radiographic features. The dental records of 946 patients from the Research and Clinical Center for Dental Trauma in Primary Teeth were studied. Two hundred and fifty PC-traumatized upper deciduous incisors were detected. According to radiographic analysis of the records, 62.5% showed diffuse calcification, 36.3% tube-like calcification, and 1.2% concentric calcification. According to the extension of pulp calcification, the records showed: 80% partial calcification, 17.2% total coronal calcification and partial radicular calcification, and 2.8 % total coronal and radicular calcification. As for location, only 2.4% were on the coronal pulp, 5.2% on the radicular pulp and 92.4% on both radicular and coronal pulp. Regarding coronal discoloration, 54% were yellow and 2% gray. In relation to periradicular changes, 10% showed widened periodontal ligament space, 3.1% internal resorption, 10% external resorption, 10.4% periapical bone rarefaction. Since PC is a general term, it is important to classify it and correlate it to clinical and radiographic changes, in order to establish the correct diagnosis, treatment and prognosis of each case.

  17. Early Experiences with Node-Level Power Capping on the Cray XC40 Platform

    Energy Technology Data Exchange (ETDEWEB)

    Pedretti, Kevin; Olivier, Stephen Lecler; Ferreira, Kurt Brian; Shipman, Galen; Shu, Wei

    2015-10-01

    Power consumption of extreme-scale supercomputers has become a key performance bottleneck. Yet current practices do not leverage power management opportunities, instead running at ''maximum power''. This is not sustainable. Future systems will need to manage power as a critical resource, directing where it has greatest benefit. Power capping is one mechanism for managing power budgets, however its behavior is not well understood. This paper presents an empirical evaluation of several key HPC workloads running under a power cap on a Cray XC40 system, and provides a comparison of this technique with p-state control, demonstrating the performance differences of each. These results show: 1. Maximum performance requires ensuring the cap is not reached; 2. Performance slowdown under a cap can be attributed to cascading delays which result in unsynchronized performance variability across nodes; and, 3. Due to lag in reaction time, considerable time is spent operating above the set cap. This work provides a timely and much needed comparison of HPC application performance under a power cap and attempts to enable users and system administrators to understand how to best optimize application performance on power-constrained HPC systems.

  18. Anthraquinone-A Review of the Rise and Fall of a Pulping Catalyst

    Science.gov (United States)

    Peter W. Hart; Alan W. Rudie

    2014-01-01

    The application of anthraquinone (AQ) as a pulping catalyst has been well documented in scientific studies and mill applications. AQ is known to increase the rate of delignification, enabling a reduction in pulping time, temperature, or chemical charge and an increase in pulp yield. Specific details of AQ use are not extensively reviewed in this work. The review...

  19. Pulp Inflammation Diagnosis from Clinical to Inflammatory Mediators: A Systematic Review.

    Science.gov (United States)

    Zanini, Marjorie; Meyer, Elisabeth; Simon, Stéphane

    2017-07-01

    Similar to other tissues, the dental pulp mounts an inflammatory reaction as a way to eliminate pathogens and stimulate repair. Pulp inflammation is prerequisite for dentin pulp complex repair and regeneration; otherwise, chronic disease or pulp necrosis occurs. Evaluation of pulp inflammation severity is necessary to predict the clinical success of maintaining pulp vitality. Clinical limitations to evaluating in situ inflammatory status are well-described. A molecular approach that aids clinical distinction between reversible and irreversible pulpitis could improve the success rate of vital pulp therapy. The aim of this article is to review inflammatory mediator expression in the context of clinical diagnosis. We searched PubMed and Cochrane databases for articles published between 1970 and December 2016. Only published studies of inflammatory mediator expression related to clinical diagnosis were eligible for inclusion and analysis. Thirty-two articles were analyzed. Two molecular approaches were described by study methods, protein expression analysis and gene expression analysis. Our review indicates that interleukin-8, matrix metalloproteinase 9, tumor necrosis factor-α, and receptor for advanced glycation end products expression increase at both the gene and protein levels during inflammation. Clinical irreversible pulpitis is related to specific levels of inflammatory mediator expression. The difference in expression between reversible and irreversible disease is both quantitative and qualitative. On the basis of our analysis, in situ quantification of inflammatory mediators may aid in the clinical distinction between reversible and irreversible pulpitis. Copyright © 2017 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  20. Investigation on anatomical, chemical and pulping characteristics of Silybum marianum stem

    Directory of Open Access Journals (Sweden)

    Rahim Yadollahi

    2013-11-01

    Full Text Available To study the possibility of using plant Silybum marianum in paper industry, its stem yield potential, chemical properties, fiber indices and pulping was evaluated. Mean value of fiber length and diameter of Silybum marianumstem harvested in early June, were calculated 178 and 3.5 cm, respectively. Dry weight of whole stem determined 4710 kg per hectar. Mean value of fiber length, diameter, cavity diameter and wall thickness were obtained 1194, 16.06, 9.06, and 3.66 μm, respectively. Raunkel, flexibility, and slenderness ratios of its fibers were determined 80.83, 56.39, and 74.37, respectively. Results of chemical analysis showed that the stem comprises %70.35 holocellulose, %39.25 cellulose, %13.13 lignin, %3.09 acetone-soluble, %18 hot water-soluble extractives, and %11/85 ash. Soda pulp was obtained at 165 °C pulping time, 25% alkaline and 10:1 ratio of liquor to chips. Results of pulping showed that with increasing of pulping time from 30 to 210 minutes, the kappa number and yield decreased from 77.32 to 32.13 and 43.6 to 36.5, respectively; whereas accepted pulp yield (passed from 20 mesh increased from 17.4 to 35.5% paper made of pulp with kappa number 32 had 36.82 Nm/g tensile index, 3.76 km breaking length and 1.75 Kpa.m2/g burst strength. In general, achieved results have shown that the present studied species could be regarded more for papermaking because of the suitable fiber biometrical and chemical properties as compared to other non-woody plants.

  1. NATURE MANAGEMENT, LANDSCAPE AND THE CAP

    OpenAIRE

    Brouwer, Floor M.; Godeschalk, Frans E.

    2004-01-01

    The integration of nature management, landscape and environmental concerns into the Common Agricultural Policy (CAP) has gained momentum with the CAP reforms adopted in June 2003. The report explores instruments and approaches that contribute to the inte-gration of nature conservation and landscape concerns into the CAP. A broader use of the CAP instruments might help to achieve nature types in the Netherlands.

  2. Effect of Flotation Time on the Deinking Process and Properties of Deinked Pulp

    Directory of Open Access Journals (Sweden)

    Sami İmamoğlu

    2012-11-01

    Full Text Available This paper discusses the influence of flotation time on the ink removal and physical properties of resulting deinked pulp. Flotation deinking of high quality office paper, printed in a digital duplicating machine based on screen-printing system, was studied using a laboratory cell with a nominal capacity of 10 L. Formamidine sulfinic acid was used in a pulper and pulping conditions such as temperature, retention time, chemical dosage and consistency were held constant prior to flotation process. During the flotation process while pulp consistency, agitation speed, air flow rate and other conditions keeping constant, only flotation time were analyzed in terms of physical properties of resulting pulp quality, pulp yield, filler and fibre balance and waste water quality aspects at 0, 3, 6, 9, 12 and 15 minutes. Increased flotation time substantially affected optical properties of pulp up to some point then became steady. Yield losses increased as might be expected with increasing flotation time by the 9th minute as flotation sludge skimmed off on top of the flotation cell. Filler content of flotation sludge increased with increasing flotation time while fibre content was decreasing to a certain extent. Thanks to removal of ink and filler materials from pulp physical properties of evaluated handsheets enhanced. The overall results demonstrate that increasing flotation time increased physical and optical properties of resulting pulp. On the other hand flotation time should be increased up to critical point. Exceeding critical point increased yield losses, consumed time and energy without gaining significant pulp qualities.

  3. Characteristics and utilization of non-wood pulp and paper; Himokuzai pulp / kami no tokucho to sono riyo

    Energy Technology Data Exchange (ETDEWEB)

    Hara, H. [Mishima Paper Co. Ltd., Shizuoka (Japan)

    1998-09-01

    The reasons, difficulty in the usage, and the methods of use of non-wood papers are discussed. Non-wood papers sold in Japan are arranged basing on published data. The type of non-wood paper classified as special printing paper makes up the majority, and there are a variety of other products such as printing board, coated paper, thin paper, wrapping paper, functional paper, and watercolor paper. Kenaf and cotton are used in large quantities as non-wood plants for paper production, and use of bagasse and bamboo is increasing. Non-wood paper are used in consideration of environmental and resources problems and for utilizing the special features of non-wood fiber, and the characteristics of non-wood pulp and the effect of non-wood paper are discussed in this report. It is expected that papers utilizing the characteristics of non-wood paper will be developed. Non-wood papers are substitutions for various papers produced from wood pulp, and the fundamental point is the method of improving the original quality and paper quality by combining with non-wood pulp. 11 refs., 4 figs., 3 tabs.

  4. CAPS Simulation Environment Development

    Science.gov (United States)

    Murphy, Douglas G.; Hoffman, James A.

    2005-01-01

    The final design for an effective Comet/Asteroid Protection System (CAPS) will likely come after a number of competing designs have been simulated and evaluated. Because of the large number of design parameters involved in a system capable of detecting an object, accurately determining its orbit, and diverting the impact threat, a comprehensive simulation environment will be an extremely valuable tool for the CAPS designers. A successful simulation/design tool will aid the user in identifying the critical parameters in the system and eventually allow for automatic optimization of the design once the relationships of the key parameters are understood. A CAPS configuration will consist of space-based detectors whose purpose is to scan the celestial sphere in search of objects likely to make a close approach to Earth and to determine with the greatest possible accuracy the orbits of those objects. Other components of a CAPS configuration may include systems for modifying the orbits of approaching objects, either for the purpose of preventing a collision or for positioning the object into an orbit where it can be studied or used as a mineral resource. The Synergistic Engineering Environment (SEE) is a space-systems design, evaluation, and visualization software tool being leveraged to simulate these aspects of the CAPS study. The long-term goal of the SEE is to provide capabilities to allow the user to build and compare various CAPS designs by running end-to-end simulations that encompass the scanning phase, the orbit determination phase, and the orbit modification phase of a given scenario. Herein, a brief description of the expected simulation phases is provided, the current status and available features of the SEE software system is reported, and examples are shown of how the system is used to build and evaluate a CAPS detection design. Conclusions and the roadmap for future development of the SEE are also presented.

  5. Stimulation of angiogenesis, neurogenesis and regeneration by side population cells from dental pulp.

    Science.gov (United States)

    Ishizaka, Ryo; Hayashi, Yuki; Iohara, Koichiro; Sugiyama, Masahiko; Murakami, Masashi; Yamamoto, Tsubasa; Fukuta, Osamu; Nakashima, Misako

    2013-03-01

    Mesenchymal stem cells (MSCs) have been used for cell therapy in various experimental disease models. However, the regenerative potential of MSCs from different tissue sources and the influence of the tissue niche have not been investigated. In this study, we compared the regenerative potential of dental pulp, bone marrow and adipose tissue-derived CD31(-) side population (SP) cells isolated from an individual porcine source. Pulp CD31(-) SP cells expressed the highest levels of angiogenic/neurotrophic factors and had the highest migration activity. Conditioned medium from pulp CD31(-) SP cells produced potent anti-apoptotic activity and neurite outgrowth, compared to those from bone marrow and adipose CD31(-) SP cells. Transplantation of pulp CD31(-) SP cells in a mouse hindlimb ischemia model produced higher blood flow and capillary density than transplantation of bone marrow and adipose CD31(-) SP cells. Motor function recovery and infarct size reduction were greater with pulp CD31(-) SP cells. Pulp CD31(-) SP cells induced maximal angiogenesis, neurogenesis and pulp regeneration in ectopic transplantation models compared to other tissue sources. These results demonstrate that pulp stem cells have higher angiogenic, neurogenic and regenerative potential and may therefore be superior to bone marrow and adipose stem cells for cell therapy. Copyright © 2012 Elsevier Ltd. All rights reserved.

  6. The cervical cap (image)

    Science.gov (United States)

    The cervical cap is a flexible rubber cup-like device that is filled with spermicide and self-inserted over the cervix ... left in place several hours after intercourse. The cap is a prescribed device fitted by a health ...

  7. Effect of the addition of collagen in the preparation of hydroxyapatite, aiming the application of pulp capping

    International Nuclear Information System (INIS)

    Ribeiro, Geysy L.; Mendes, Luis C.

    2011-01-01

    This work studied the action of collagen (COLL) in the hydroxyapatite (HA) synthesis, produced through sol-gel process, in order to mimetize the chemical composition of dental tissue. The resulting material was characterized by energy dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), wide-angle X-ray diffraction (WAXD) and scanning electron microscopy (SEM). The Ca/P ratio was determined through EDX - 1,89 e 2,38, with and without collagen, respectively. The FT-IR analysis showed no significant interaction between the constituents of the composite. The R-ray diffractograms indicated an increase of the resolution and intensity of the HA peak. The photomicrographies showed that the preparation method exhibited significantly influence onto the hydroxyapatite morphology, as well as resulted in a homogeneously dispersed composite. (author)

  8. Does uncertainty justify intensity emission caps?

    International Nuclear Information System (INIS)

    Quirion, Philippe

    2005-01-01

    Environmental policies often set 'relative' or 'intensity' emission caps, i.e. emission limits proportional to the polluting firm's output. One of the arguments put forth in favour of relative caps is based on the uncertainty on business-as-usual output: if the firm's production level is higher than expected, so will be business-as-usual emissions, hence reaching a given level of emissions will be more costly than expected. As a consequence, it is argued, a higher emission level should be allowed if the production level is more important than expected. We assess this argument with a stochastic analytical model featuring two random variables: the business-as-usual emission level, proportional to output, and the slope of the marginal abatement cost curve. We compare the relative cap to an absolute cap and to a price instrument, in terms of welfare impact. It turns out that in most plausible cases, either a price instrument or an absolute cap yields a higher expected welfare than a relative cap. Quantitatively, the difference in expected welfare is typically very small between the absolute and the relative cap but may be significant between the relative cap and the price instrument. (author)

  9. Nutritional composition of Annona crassiflora pulp and acceptability of bakery products prepared with its flour

    Directory of Open Access Journals (Sweden)

    Paula Villela

    2013-09-01

    Full Text Available Annona crassiflora offers an edible fruit native to the Savanna. This study aimed to develop a flour meal from Annona crassiflora pulp; analyze the chemical composition of the fresh pulp and its flour; develop and verify the acceptance of formulations with different concentrations of the flour of Annona crassiflora pulp. Fruit used were selected and processed. The pulp was dried in an oven at 60-65 ºC/48h. We analyzed the chemical composition, and two formulations of breads were prepared with 10 and 20% Annona crassiflora pulp. The results showed that the drying of Annona crassiflora pulp enriched its nutritional value. The Annona crassiflora pulp showed important chemical components, as insoluble fibers (pulp and flour, minerals (potassium, calcium, manganese and others and antioxidant compounds. The formulations were well-accepted in a sensory point of view and proved to be a good alternative to the exploitation of the fruit.

  10. Optical and Physical Properties of ONP Deinked Pulp

    Directory of Open Access Journals (Sweden)

    Iman Akbarpoor

    2012-01-01

    Full Text Available Enzymes are protein molecules with complex structures that accelerate the biochemical reactions. Activity of these chemical compounds is accomplished at limited range of pH, temperature and concentration. In this study, the effects of different concentrations of cellulose enzyme were investigated on deinking of old newsprint. Old newsprint (ONP was repulped at 5% consistency for 10 minutes in disintegrator with total revolution number of 26500. Enzymatic treatments of recycled ONP pulp were done under constant conditions (10% consistency,treatment time of 15 minutes, pH range of 5-5.5 at different cellulose concentrations of 0.025, 0.05, 0.1 and 0.2% (based on oven-dry waste paper. The optical and physical properties of the standard paper (60g/m2 made at different concentrations of cellulose were evaluated in comparison with control pulp (untreated ONP pulp with cellulase. Overall, the results achieved by comparison the optical properties of the paper produced indicated that using cellulase in deinking of ONP led to increase the brightness and the yellowness and decrease the opacity. The brightness was improved to a maximum level of 47.5 ISO %, but the yellowness was decreased to a minimum level of 11.3 ISO %, while the brightness reduced and the yellowness increased at higher concentrations than 0.05% cellulase. The highest opacity of 99.3 ISO % was achieved using 0.1% cellulase even higher than control pulp. The results gained by comparison the physical properties of the paper showed that using cellulase resulted in decrease of paper calliper, air resistance and density and improve the freeness of pulp

  11. Betatron activation analysis of cupriferous flotation pulp

    International Nuclear Information System (INIS)

    Kaminski, R.; Matenko, J.; Mencel, J.; Janiczek, J.; Kielsznia, J.

    1974-01-01

    A method of copper determination in cupriferous flotation pulp by photo-activation analysis using betatron and another equipments of existent ''analytical line'' intended for copper determination in dry samples has been described. An activation has been achieved with 14.9 MeV γ-photons. The excitation activity was investigated by using two scintillation detectors and a fast coincidence circuit with resolution time 80 ns. The precision of method was determined as +- 4.25% in 0.95 confidence level for pulp with concentration 5% Cu and +- 24% for 0.06% Cu. (author)

  12. Banana (Musa spp) from peel to pulp: ethnopharmacology, source of bioactive compounds and its relevance for human health.

    Science.gov (United States)

    Pereira, Aline; Maraschin, Marcelo

    2015-02-03

    Banana is a fruit with nutritional properties and also with acclaimed therapeutic uses, cultivated widely throughout the tropics as source of food and income for people. Banana peel is known by its local and traditional use to promote wound healing mainly from burns and to help overcome or prevent a substantial number of illnesses, as depression. This review critically assessed the phytochemical properties and biological activities of Musa spp fruit pulp and peel. A survey on the literature on banana (Musa spp, Musaceae) covering its botanical classification and nomenclature, as well as the local and traditional use of its pulp and peel was performed. Besides, the current state of art on banana fruit pulp and peel as interesting complex matrices sources of high-value compounds from secondary metabolism was also approached. Dessert bananas and plantains are systematic classified into four sections, Eumusa, Rhodochlamys, Australimusa, and Callimusa, according to the number of chromosomes. The fruits differ only in their ploidy arrangement and a single scientific name can be given to all the edible bananas, i.e., Musa spp. The chemical composition of banana's peel and pulp comprise mostly carotenoids, phenolic compounds, and biogenic amines. The biological potential of those biomasses is directly related to their chemical composition, particularly as pro-vitamin A supplementation, as potential antioxidants attributed to their phenolic constituents, as well as in the treatment of Parkinson's disease considering their contents in l-dopa and dopamine. Banana's pulp and peel can be used as natural sources of antioxidants and pro-vitamin A due to their contents in carotenoids, phenolics, and amine compounds, for instance. For the development of a phytomedicine or even an allopathic medicine, e.g., banana fruit pulp and peel could be of interest as raw materials riches in beneficial bioactive compounds. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  13. Cryopyrin-Associated Autoinflammatory Syndromes (CAPS) - Juvenile

    Science.gov (United States)

    ... all ethnic groups can be affected. What are CAPS? Cryopyrin-associated autoinflammatory syndromes (CAPS) consist of three ... ears by magnetic resonance imaging (MRI). How is CAPS treated? Medications that target interleukin-1 are very ...

  14. Effect of Partial Pre-Extraction of Hemicelluloses on the Properties of Pinus radiata Chemimechanical Pulps

    Directory of Open Access Journals (Sweden)

    Pablo Reyes

    2015-09-01

    Full Text Available Extraction of hemicelluloses prior to pulping and conversion of the extracted hemicelluloses to other bioproducts could provide additional revenue to traditional pulp and paper industries. The effect of hemicelluloses pre-extraction with a hydrothermal (HT process on Pinus radiata chemimechanical pulp (CMP properties was investigated in this study. The HT extraction resulted in a release of 7% to 58% of the initial amount of hemicelluloses from the wood. The extraction yield increased with temperature and extraction time. This hemicellulosic fraction was in the form of low molar mass oligomers with molecular weights varying from 1.5 to 100 kDa. Compared with the control (unextracted CMP pulp, the HT pre-extraction significantly reduced the refining energy to obtain a given fibrillation degree (freeness. The pulp yield with the HT/CMP process was in the range of 56% to 75%. Fiber properties of the pulps from pre-extracted wood, such as fiber length, were reduced, while increases in fiber width, fines content, fiber coarseness, and kink index were observed in comparison with the control pulps. The strength properties of CMP pulps decreased with increasing amounts of hemicellulose removal during the stage prior to pulping.

  15. Differentiation ability of rat postnatal dental pulp cells in vitro.

    NARCIS (Netherlands)

    Zhang, W.; Walboomers, X.F.; Wolke, J.G.C.; Bian, Z.; Fan, M.W.; Jansen, J.A.

    2005-01-01

    The current rapid progression in stem cell research has enhanced our knowledge of dental tissue regeneration. In this study, rat dental pulp cells were isolated and their differentiation ability was evaluated. First, dental pulp cells were obtained from maxillary incisors of male Wistar rats.

  16. Method for Forming Pulp Fibre Yarns Developed by a Design-driven Process

    Directory of Open Access Journals (Sweden)

    Tiia-Maria Tenhunen

    2016-01-01

    Full Text Available A simple and inexpensive method for producing water-stable pulp fibre yarns using a deep eutectic mixture composed of choline chloride and urea (ChCl/urea was developed in this work. Deep eutectic solvents (DESs are eutectic mixtures consisting of two or more components that together have a lower melting point than the individual components. DESs have been previously studied with respect to cellulose dissolution, functionalisation, and pre-treatment. This new method uses a mixture of choline chloride and urea, which is used as a swelling and dispersing agent for the pulp fibres in the yarn-forming process. Although the pulp seemed to form a gel when dispersed in ChCl/urea, the ultrastructure of the pulp was not affected. To enable water stability, pulp fibres were crosslinked by esterification using polyacrylic acid. ChCl/urea could be easily recycled and reused by distillation. The novel process described in this study enables utilisation of pulp fibres in textile production without modification or dissolution and shortening of the textile value chain. An interdisciplinary approach was used, where potential applications were explored simultaneously with material development from process development to the early phase prototyping.

  17. The North Zealand CAP Monitor

    DEFF Research Database (Denmark)

    Nielsen, Minna; Ravn, Pernille; Notander Clausen, Lise

    with CAP. We started with 34 audit variables. Through repeated cycles of testing, feedback and discussions, we reduced the number of indicators to 22 and time per audit from 20 to 10 minutes. Strategy for change To link the monitoring system with our patient pathway for CAP we established an improvement...... Designing a database Designing and testing a dashboard to present indicators in a balanced way Messages for others Auditing patients with a common disease as CAP is useful to identify areas for improvement for a large group of patients. The baseline audit can serve as a basis for a monitoring system......Contect We describe how we developed a monitoring system for community acquired pneumonia (CAP) at North Zealand Regional hospital. We serve 310.000 inhabitants and annually around 3200 patients with CAP are admitted. As part of a program of clinical pathways for common conditions, a pathway...

  18. Aging of in vitro pulp illustrates change of inflammation and dentinogenesis.

    Science.gov (United States)

    Lee, Young-Hee; Kim, Go-Eun; Cho, Hye-Jin; Yu, Mi-Kyoung; Bhattarai, Govinda; Lee, Nan-Hee; Yi, Ho-Keun

    2013-03-01

    Dental pulp functions include pulp cell activity involvement in dentin formation. In this study we investigated the age-related changes in dental pulp cells that may influence pulp cell activity for restoring pulp function. Human dental pulp cells (HDPCs) were serially subcultured until spontaneously arrested. Altered expression of chronic inflammatory molecules and age-related molecules were determined by Western blotting. Odontogenic functions impaired by senescence were assayed by Western blotting, reverse transcriptase polymerase chain reaction, alkaline phosphatase activity, and alizarin red S staining. To understand the mechanism of aging process by stress-induced premature senescence (SIPS), the cells were treated with H(2)O(2). Replicative senescence and SIPS were also compared. Replicative senescence of HDPCs was characterized by senescence-associated β-galactosidase activity and reactive oxygen species formation. These cells exhibited altered expression of chronic inflammatory molecules such as intracellular adhesion molecule-1, vascular cell adhesion molecule-1, peroxisome proliferator activated receptor-gamma, and heme oxygenase-1 and age-related molecules such as p53, p21, phosphorylated-extracellular signal-regulated kinase, and c-myb. SIPS cell results were similar to replicative senescence. Furthermore, HDPCs decreased odontogenic markers such as dentin sialophosphoprotein and dentin matrix-1 and osteogenic markers such as bone morphogenetic protein-2 and -7, runt-related transcription factor-2, osteopontin, alkaline phosphatase activity, and mineralized nodule formation by replicative senescence and SIPS. This study suggests that development of aging-related molecules in pulp cells offers understanding of cellular mechanisms and biological events responsible for tooth preservation and maintenance strategies for healthy teeth across the life span. Copyright © 2013 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  19. Quality evaluation of dissolving pulp fabricated from banana plant stem and its potential for biorefinery.

    Science.gov (United States)

    Das, Atanu Kumar; Nakagawa-Izumi, Akiko; Ohi, Hiroshi

    2016-08-20

    The study was conducted to evaluate the quality of dissolving pulp of Musa sapientum L. (banana) plant stem and its potential for biorefinery. Introduction of pre-hydrolysis prior to any alkaline pulping process helps to reduce the content of hemicellulose and consequently produce acceptably high content of cellulose pulp. Water pre-hydrolysis was done at 150°C for 90min. The amount of lignin, xylan and glucan in the extracted pre-hydrolysis liquor (PHL) was 1.6, 4.9 and 1.6%, respectively. Pulping of pre-extracted chips was done following soda-AQ, alkaline sulfite and kraft process. The ratio of chip to liquor was 1:7 for both pre-hydrolysis and pulping. The kraft pulping process with 20% active alkali and 25% sulfidity at 150°C for 90min showed the best result. The lowest kappa number was 26.2 with a considerable pulp yield of 32.7%. The pulp was bleached by acidic NaClO2 and the consistency was 10% based on air-dried pulp. The lowest amount of 7% NaClO2 was used for the bleaching sequence of D0ED1ED2. After D0ED1ED2 bleaching, the pulp showed that α-cellulose, brightness and ash were 91.9, 77.9 and 1.6% respectively. The viscosity was 19.9cP. Hence, there is a possibility to use banana plant stem as a raw material for dissolving grade pulp and other bioproducts. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Integrating black liquor gasification with pulping - Process simulation, economics and potential benefits

    Science.gov (United States)

    Lindstrom, Erik Vilhelm Mathias

    Gasification of black liquor could drastically increase the flexibility and improve the profit potential of a mature industry. The completed work was focused on research around the economics and benefits of its implementation, utilizing laboratory pulping experiments and process simulation. The separation of sodium and sulfur achieved through gasification of recovered black liquor, can be utilized in processes like modified continuous cooking, split sulfidity and green liquor pretreatment pulping, and polysulfide-anthraquinone pulping, to improve pulp yield and properties. Laboratory pulping protocols have been developed for these modified pulping technologies and different process options evaluated. The process simulation work around BLG has led to the development of a WinGEMS module for the low temperature MTCI steam reforming process, and case studies comparing a simulated conventional kraft process to different process options built around the implementation of a BLG unit operation into the kraft recovery cycle. Pulp yield increases of 1-3% points with improved product quality, and the potential for capital and operating cost savings relative to the conventional kraft process have been demonstrated. Process simulation work has shown that the net variable operating cost for a pulping process using BLGCC is highly dependent on the cost of lime kiln fuel and the selling price of green power to the grid. Under the assumptions taken in the performed case study, the BLGCC process combined with split sulfidity or PSAQ pulping operations had net variable operating cost 2-4% greater than the kraft reference. The influence of the sales price of power to the grid is the most significant cost factor. If a sales price increase to 6 ¢/KWh for green power could be achieved, cost savings of about $40/ODtP could be realized in all investigated BLG processes. Other alternatives to improve the process economics around BLG would be to modify or eliminate the lime kiln unit

  1. Evaluation of Pentachlorophenol Residues in Some Hygienic Papers Prepared from Virgin and Secondary Pulp by Electron Capture Gas Chromatographic Method

    Directory of Open Access Journals (Sweden)

    Behrouz Akbari-adergani

    2016-01-01

    Full Text Available In this study, residual amount of pentachlorophenol (PCP as the most important paper preservative, which is extremely hazardous pollutant, was determined in some tissue papers and napkins. Twenty-five samples of two producing hygienic paper factories prepared from virgin and secondary pulp were analyzed for the presence of trace amount of PCP. The analytical procedure involved direct extraction of PCP from hygienic paper and its determination by gas chromatography with electron capture detection. The statistical results for the analysis of all samples revealed that there were significant differences between mean of PCP in hygienic papers prepared from virgin and secondary pulp (P<0.05. This method gave recoveries of 86-98% for hygienic paper made from virgin pulp and 79-92% for hygienic paper made from secondary pulp. The limit of detection (LOD and limit of quantification (LOQ for PCP were 6.3 and 21.0 mg/kg, respectively. The analytical method has the requisite sensitivity, accuracy, precision and specificity to assay PCP in hygienic papers. This study demonstrates a concern with exposition to PCP considering that hygienic paper is largely consumed in the society.

  2. Application of resin in pulp technique for ion exchange separation of uranium from alkaline leachate

    International Nuclear Information System (INIS)

    Sreenivas, T.; Rajan, K.; Chakravorty, J.

    2014-01-01

    The hydrometallurgical process for the recovery of uranium from different ores uses ion exchange (IX) technique for the separation of dissolved uranium values. Conventionally, the IX process is carried out on leach solution obtained after the filtration or counter-current decantation of the leach slurries. Amongst the two types of leach pulps generated in uranium ore processing, viz acidic and alkaline, the latter one consists of predominantly fine-size pulps of higher viscosity, thus making the solid-liquid separation an arduous task. Sustained research for improvising the efficiency of various unit operations in the uranium process flowsheet have resulted in advent of new generation resins which are mechanically re-silent, posses higher exchange capacity thereby enabling separation of dissolved uranium ions from the leach pulps directly. Some of the prominent low-grade uranium ore deposits in India are hosted in acid consuming gangue matrix. These ore deposits necessitate fine grinding as well as application of alkaline leaching for the dissolution of uranium values. The leach pulps analyse 500 – 600 mg/l of U3O8 and contain total dissolved solutes (TDS) to the extent of about 50 g/l. Analysis of the characteristics of the leach pulp indicated suitability of resin-in-pulp technique for the separation of uranyl carbonate anions from the leachate. This paper describes the results of the RIP test work on alkaline leach slurry using various commercially available strong base anionic exchange resins. Parametric variation studies were conducted to establish the adsorption isotherm and sorption kinetics followed by elution of loaded uranium. Based on these results semi-continuous experiments on “carousel” mode were carried out. The results indicate superiority of gel type polystyrene based resins grafted with quaternary ammonium ion in comparison to the macro-porous resins. Semi-continuous counter-current extraction and elution tests indicated that about 98% of

  3. Drying kinetics of atemoya pulp

    Directory of Open Access Journals (Sweden)

    Plúvia O. Galdino

    Full Text Available ABSTRACT This study was conducted in order to obtain drying curves of whole atemoya pulp through the foam-mat drying method. The suspension was prepared with whole atemoya pulp mixed with 2% of Emustab® and 2% of Super Liga Neutra® with mixing time of 20 min, and dried in a forced-air oven at different temperatures (60; 70 and 80 °C and thicknesses of the foam layer (0.5, 1.0 and 1.5 cm. The drying rate curves were plotted against the water content ratio and the semi-theoretical models of Henderson & Pabis, Page and Midilli were used. All tested models showed coefficient of determination (R2 above 0.993, and the Midilli model showed the best fit for all conditions. Drying curves were affected by temperature and layer thickness.

  4. Hydrogen production from carrot pulp by the extreme thermophiles Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana

    NARCIS (Netherlands)

    Vrije, de G.J.; Budde, M.A.W.; Lips, S.J.J.; Bakker, R.R.; Mars, A.E.; Claassen, P.A.M.

    2010-01-01

    Hydrogen was produced from carrot pulp hydrolysate, untreated carrot pulp and (mixtures of) glucose and fructose by the extreme thermophiles Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana in pH-controlled bioreactors. Carrot pulp hydrolysate was obtained after enzymatic hydrolysis

  5. A LARCH BIOREFINERY: INFLUENCE OF WASHING AND PS CHARGE ON PRE-EXTRACTION PSAQ PULPING

    Directory of Open Access Journals (Sweden)

    Hanna S. Hörhammer

    2012-06-01

    Full Text Available This study deals with a biorefinery concept based on larch wood. Wood chips of Siberian larch (Larix sibirica Lebed. were treated with water before pulping at the optimal pre-extraction (PE condition of 150 °C and 90 minutes. Through PE, about 12.4% of the wood mass is dissolved, mainly from the arabinogalactan hemicellulose component. Fermentation of the hemicellulose-rich larch extract with Bacillus coagulans resulted in consumption of all C6 and C5 sugars and produced lactic acid in high yield. PE before pulping resulted in lower (4 to 5% pulp yield than for control kraft pulps. However, the pulp yield loss may be reduced by addition of polysulfide (PS and anthraquinone (AQ. The present study focuses on the effect of the degree of washing of the extracted chips and that of the PS charge in PSAQ pulping on the final properties of the pulp. Three different levels of washing and three different PS charges were tested. The characteristics of the extract, wash water, pulp, and black liquor samples were determined. The amount of sugars in the combined stream of collected extract and wash water obtained by mild washing was 10.2% on o.d. wood.

  6. Fiber length and pulping characteristics of switchgrass, alfalfa stems, hybrid poplar and willow biomasses.

    Science.gov (United States)

    Ai, Jun; Tschirner, Ulrike

    2010-01-01

    Switchgrass (Panicum virgatum), alfalfa stems (Medicago sativa), second year growth hybrid poplar (Populus) and willow (Salix spp.) were examined to determine fiber characteristics, pulping behavior and paper properties. Alfalfa stems and switchgrass both showed length weighted average fiber length (LWW) of 0.78 mm, a very narrow fiber length distribution and high fines content. Willow and hybrid poplar have lower fines content but a very low average fiber length (0.42 and 0.48 mm LWW). In addition, the four biomass species showed distinctly different chemical compositions. Switchgrass was defibered successfully using Soda and Soda Anthraquinone (AQ) pulping and demonstrated good paper properties. Both fast-growing wood species pulped well using the Kraft process, and showed acceptable tensile strength, but low tear strength. Alfalfa stems reacted very poorly to Soda and Soda AQ pulping but responded well to Kraft and Kraft AQ. Pulps with tensile and tear strength considerably higher than those found for commercial aspen pulps were observed for alfalfa. All four biomass species examined demonstrated low pulp yield. The highest yields were obtained with poplar and switchgrass (around 43%). Considering the short fibers and low yields, all four biomass types will likely only be used in paper manufacturing if they offer considerable economic advantage over traditional pulp wood.

  7. Effects of the bleaching sequence on the optical brighteners action in eucalyptus kraft pulp

    Directory of Open Access Journals (Sweden)

    Mauro Manfredi

    2014-06-01

    Full Text Available During the bleaching process the pulp is treated with chemical reagents that can be retained in the pulp and interfere in the action of the optical brighteners. Different bleaching sequences can produce pulps at the same brightness but with different potential to whiteness increase when treated with optical brighteners. The objective of this study was to evaluate the influence of the bleaching sequence on the efficiency of disulphonated and tetrasulphonated optical brighteners. Eucalyptus kraft pulp was bleached using four different bleaching sequences. For each pulp three brightness targets were aimeds. For each bleaching sequence mathematical model was generated for predicting the final pulp whiteness according to the initial brightness and the optical brightener charge applied. The presence of organochlorine residues in the pulp reduced the effectiveness of the optical brighteners. Therefore, bleaching sequences that use low chlorine dioxide charge favors for greater gains in whiteness with the application of optical brighteners. The replacement of the final chlorine dioxide bleaching stage with a hydrogen peroxide one in the sequence increased the efficiency of the optical brightening agents.

  8. Timing of pulp extirpation for replanted avulsed teeth.

    LENUS (Irish Health Repository)

    Stewart, Chris

    2009-01-01

    A search was performed (April 2004) across four databases, namely Ovid Medline, Cochrane Library, PubMed and Web of Science, relevant to the proposed PICO ( Patient or problem, Intervention, Comparison, Outcome) question: (P) for a replanted avulsed permanent tooth, (I) is early pulp extirpation within 10-14 days of replantation, (C) compared with delayed pulp extirpation, (O) associated an increased likelihood of successful periodontal healing after tooth replantation. Only articles published in the English language were considered.

  9. Effects of simvastain and enamel matrix derivative on Portland cement with bismuth oxide-induced growth and odontoblastic differentiation in human dental pulp cells.

    Science.gov (United States)

    Lee, So-Youn; Min, Kyung-San; Choi, Gi-Woon; Park, Jae-Hong; Park, Sang-Hyuk; Lee, Sang-Im; Kim, Eun-Cheol

    2012-03-01

    We previously reported that bismuth oxide containing Portland cement (BPC) showed similar biocompatibility to Portland cement (PC) in periodontal ligament cells. However, the bioactivity of simvastatin and Emdogain (Biora AB, Malmö, Sweden) on BPC was not reported. The aim of this study was to evaluate the effects of simvastatin and Emdogain on BPC compared with mineral trioxide aggregate (MTA) in human dental pulp cells (HDPCs). Cell growth was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay. Differentiation was evaluated by alkaline phosphatase (ALP) activity, alizarin red staining, and reverse-transcriptase polymerase chain reaction. The cell growth of HDPCs exposed to Emdogain and simvastatin plus BPC was superior to those administered BPC alone and similar to those that received MTA for 14 days. The simvastatin and Emdogain groups increased the odontogenic potential of the BPC group with respect to ALP activity, mineralization nodules, messenger RNA expression of ALP, osteopontin, osteocalcin, Runx2, and osterix. These results suggest that simvastatin and Emdogain improved cell growth and the differentiation of the BPC group in HDPCs and may be useful ingredients in BPC as pulp-capping material. Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  10. The effects of high energy radiation on the pulping properties of Pinus radiation and Eucalyptus regnans

    International Nuclear Information System (INIS)

    McLaren, K.G.; Garland, C.P.; Higgins, H.G.

    1976-01-01

    Studies have been made of the effects of high energy radiation on the pulping behaviour of Eucalyptus regnans and Pinus radiata. Pre-irradiation of wood chips with small doses of 60 Co gamma radiation (up to about 0.2 Mrad) caused little degradation of the cellulose, and had only minor effects on the kraft pulping properties of both wood species. Pulp yield, Kappa number and strength properties of the pulps showed little change. There was also little effect on the bisulphite cooking of Pinus radiata. As the dose was increased to 1 Mrad, degradation of cellulose (as indicated by degree of polymerisation measurements) became significant, and Kraft pulp yields from both woods showed small reductions. The Kappa number and physical properties of these pulps were little affected at this dose level. A gamma radiation dose of 10 Mrad produced marked depolymerisation of the cellulose, and big reductions in kraft and neutral sulphite semi-chemical pulp yields. The kraft pulps showed a much higher lignin content. Some low dose (0.15 Mrad) irradiations on thin chips were carried out with a 1 MeV electron accelerator. In contrast to comparable gamma irradiations, this treatment produced discernible changes in kraft pulping behaviour. The pulp yield, under the same cooking conditions, appears to be slightly higher, but the Lignin content of the pulp was increased. (Author)

  11. Nuclear Waste Vitrification Efficiency: Cold Cap Reactions

    International Nuclear Information System (INIS)

    Kruger, A.A.; Hrma, P.R.; Pokorny, R.

    2011-01-01

    The cost and schedule of nuclear waste treatment and immobilization are greatly affected by the rate of glass production. Various factors influence the performance of a waste-glass melter. One of the most significant, and also one of the least understood, is the process of batch melting. Studies are being conducted to gain fundamental understanding of the batch reactions, particularly those that influence the rate of melting, and models are being developed to link batch makeup and melter operation to the melting rate. Batch melting takes place within the cold cap, i.e., a batch layer floating on the surface of molten glass. The conversion of batch to glass consists of various chemical reactions, phase transitions, and diffusion-controlled processes. These include water evaporation (slurry feed contains as high as 60% water), gas evolution, the melting of salts, the formation of borate melt, reactions of borate melt with molten salts and with amorphous oxides (Fe 2 O 3 and Al 2 O 3 ), the formation of intermediate crystalline phases, the formation of a continuous glass-forming melt, the growth and collapse of primary foam, and the dissolution of residual solids. To this list we also need to add the formation of secondary foam that originates from molten glass but accumulates on the bottom of the cold cap. This study presents relevant data obtained for a high-level-waste melter feed and introduces a one-dimensional (1D) mathematical model of the cold cap as a step toward an advanced three-dimensional (3D) version for a complete model of the waste glass melter. The 1D model describes the batch-to-glass conversion within the cold cap as it progresses in a vertical direction. With constitutive equations and key parameters based on measured data, and simplified boundary conditions on the cold-cap interfaces with the glass melt and the plenum space of the melter, the model provides sensitivity analysis of the response of the cold cap to the batch makeup and melter

  12. Activity-guided identification of acetogenins as novel lipophilic antioxidants present in avocado pulp (Persea americana).

    Science.gov (United States)

    Rodríguez-Sánchez, Dariana; Silva-Platas, Christian; Rojo, Rocío P; García, Noemí; Cisneros-Zevallos, Luis; García-Rivas, Gerardo; Hernández-Brenes, Carmen

    2013-12-30

    Avocado fruit is a rich source of health-related lipophilic phytochemicals such as monounsaturated fatty acids, tocopherols, carotenes, acetogenins and sterols. However, limited information is available on the contribution of specific phytochemicals to the overall antioxidant capacity (AOC) of the fruit. Centrifugal partition chromatography was used as fractionation tool, guided by an in vitro chemical assay of oxygen radical absorbance capacity (ORAC). Subsequent experiments focused on isolation and characterization of the chemical nature of the main contributors to lipophilic AOC of avocado pulp. ORAC values obtained for acetogenins were contrasted with results from an isolated kidney mitochondria membrane lipid peroxidation bioassay. The present study established that lipophilic AOC of the pulp was significantly higher than its hydrophilic AOC. Our results confirmed the presence of acetogenins in the fractions with highest lipophilic AOC, and for the first time linked them as contributors to lipophilic-ORAC values. Further HPLC-PDA/MS-TOF analysis led to structural elucidation of two novel acetogenins, not previously reported as present in avocado pulp, along with five already known related-compounds. Antioxidant properties observed for avocado pulp acetogenins by the ORAC assay suggested that, in the presence of an emulsifying agent, acetogenins could serve as novel lipophilic antioxidants in a food matrix. Results from isolated mitochondria lipid peroxidation bioassay, indicated that L-ORAC values which may have relevance for food matrix applications, should not be interpreted to have a direct relevance in health-related claims, compounds need to be evaluated considering the complexity of biological systems. Copyright © 2013 Elsevier B.V. All rights reserved.

  13. Analysis of RNA binding by the dengue virus NS5 RNA capping enzyme.

    Directory of Open Access Journals (Sweden)

    Brittney R Henderson

    Full Text Available Flaviviruses are small, capped positive sense RNA viruses that replicate in the cytoplasm of infected cells. Dengue virus and other related flaviviruses have evolved RNA capping enzymes to form the viral RNA cap structure that protects the viral genome and directs efficient viral polyprotein translation. The N-terminal domain of NS5 possesses the methyltransferase and guanylyltransferase activities necessary for forming mature RNA cap structures. The mechanism for flavivirus guanylyltransferase activity is currently unknown, and how the capping enzyme binds its diphosphorylated RNA substrate is important for deciphering how the flavivirus guanylyltransferase functions. In this report we examine how flavivirus NS5 N-terminal capping enzymes bind to the 5' end of the viral RNA using a fluorescence polarization-based RNA binding assay. We observed that the K(D for RNA binding is approximately 200 nM Dengue, Yellow Fever, and West Nile virus capping enzymes. Removal of one or both of the 5' phosphates reduces binding affinity, indicating that the terminal phosphates contribute significantly to binding. RNA binding affinity is negatively affected by the presence of GTP or ATP and positively affected by S-adensyl methoninine (SAM. Structural superpositioning of the dengue virus capping enzyme with the Vaccinia virus VP39 protein bound to RNA suggests how the flavivirus capping enzyme may bind RNA, and mutagenesis analysis of residues in the putative RNA binding site demonstrate that several basic residues are critical for RNA binding. Several mutants show differential binding to 5' di-, mono-, and un-phosphorylated RNAs. The mode of RNA binding appears similar to that found with other methyltransferase enzymes, and a discussion of diphosphorylated RNA binding is presented.

  14. The Fractionation of Loblolly Pine Woodchips Into Pulp For Making Paper Products

    Energy Technology Data Exchange (ETDEWEB)

    Kiran Kadam, PhD

    2006-11-30

    The overall goal of the project was to test the PureVision biomass fractionation technology for making pulp from loblolly pine. A specific goal was to produce a pulp product that is comparable to pulp produced from the kraft process, while reducing the environmental effects of the kraft process, known to be a highly pollutant process. The overall goal of the project was met by using the biomass fractionation concept for making pulp product. This proof-of-concept study, done with Southern pine pinchips as feedstock, evaluated NaOH concentration and residence time as variables in single-stage cocurrent pulping process. It can be concluded that 1% NaOH is adequate for effective delignification using the PureVision process; this is about 1/3 of that used in the kraft process. Also, the PureVision process does not use sulfur-based chemicals such as N2S and hence, is environmentally more benign.

  15. Interleukin 1-beta analysis in chronically inflamed and healthy human dental pulp

    Directory of Open Access Journals (Sweden)

    Šubarić Ljiljana

    2017-01-01

    Full Text Available Background/Aim. Proinflammatory cytokines can act like endogenous pyrogen interleukin 1 (IL-1, interleukin 6 (IL-6 and tumour necrosis factor alpha (TNF α which regulate the synthesis of secondary mediators and other proinflammatory cytokines through macrophages and mesenchymal cells. They stimulate acute-phase proteins and attract inflammatory cells. The aim of this study was to determine interleukin 1-β (IL-1 β concentrations in chronically inflamed and healthy dental pulps. Methods. A total of 41 pulps (19 from patients with pulpitis chronic causa and 22 from patients with pulpatis chronic aperta, divided into two groups, were obtained from teeth with chronic pulp inflammation. The control group consisted of 12 teeth with healthy pulp. After extirpation, pulp samples were immediately placed in sterile Eppendorf tubes and frozen. After that, homogenisation was performed by a Teflon® pestle in ice-cold phosphate buffer solution at pH 7.4 whose volume was adjusted according to the weight of tissue. The supernatant was then frozen at -70°C until the performance of appropriate biochemical analyses. Cytokine IL-1 β value was determined by a commercial enzyme- linked immunosorbent assay (ELISA test. We applied the high sensitivity system technique, which may register low levels of cytokines, ranging from 0.125 to 8.0 pg/mL for IL-1 β. Results. By comparing the mean value of IL-1β, in the pulps we can see a statistically significant difference (p < 0.01 among them. The highest value of IL-1 β was in the subjects with pulpitis chronica clausa and it was 6.21 ± 2.70 pg/mL. Conclusion. Proinflammatory cytokine IL-1 β is present in detectable quantities in the pulp tissue of all vital pulps. Its highest concentrations were found in the sample group with pulpitis chronica clausa.

  16. Estimated release from the saltstone landfill effect of landfill caps and landfill-cap/monolith-liner combinations

    International Nuclear Information System (INIS)

    Wilhite, E.L.

    1985-01-01

    The effect of capping the entire saltstone landfill is dependent on the effectiveness of the clay cap in preventing infiltration. A cap that is 99% effective will reduce releases from the saltstone landfill by a factor of 7.7. Several combinations of landfill design alterations will result in meeting ground water standards

  17. Biomechanical pulping of kenaf

    Science.gov (United States)

    Aziz Ahmed; Masood Akhtar; Gary C. Myers; Gary M. Scott

    1999-01-01

    The objective of this study was to investigate the effect of fungal pretreatment of whole kenaf prior to refining on refiner electrical energy consumption, paper strength, and optical properties. We also explored the suitability of whole kenaf biomechanical pulp for making newsprint in terms of ISO brightness and strength properties. Kenaf was sterilized by autoclaving...

  18. Opposite responses of rabbit and human globin mRNAs to translational inhibition by cap analogues

    International Nuclear Information System (INIS)

    Shakin, S.H.; Liebhaber, S.A.

    1987-01-01

    The translational efficiency of an mRNA may be determined at the step of translational initiation by the efficiency of its interaction with the cap binding protein complex. To further investigate the role of these interactions in translational control, the authors compare in vitro the relative sensitivities of rabbit and human α- and β-globin mRNAs to translational inhibition by cap analogues. They find that rabbit β-globin mRNA is more resistant to translational inhibition by cap analogues than rabbit α-globin mRNA, while in contrast, human β-globin mRNA is more sensitive to cap analogue inhibition than human α- and β-globin mRNAs is unexpected as direct in vivo and in vitro comparisons of polysome profiles reveal parallel translational handling of the α- and β-globin mRNAs from these two species. This discordance between the relative translational sensitivities of these mRNAs to cap analogues and their relative ribosome loading activities suggests that cap-dependent events may not be rate limiting in steady-state globin translation

  19. Putative Stem Cells in Human Dental Pulp with Irreversible Pulpitis-An Exploratory Study

    Science.gov (United States)

    Wang, Z.; Pan, J.; Wright, JT; Bencharit, S.; Zhang, S.; Everett, ET; Teixeira, FB; Preisser, JS

    2010-01-01

    Introduction Although human dental pulp stem cells isolated from healthy teeth have been extensively characterized, it is unknown whether stem cells also exist in clinically compromised teeth with irreversible pulpitis. Here we explored whether cells retrieved from clinically compromised dental pulp have stem cell-like properties. Methods Pulp cells were isolated from healthy teeth (control group) and from teeth with clinically diagnosed irreversible pulpitis (diseased group). Cell proliferation, stem cell marker STRO-1 expression and cell odonto-osteo-genic differentiation competence were compared. Results Cells from the diseased group demonstrated decreased colony formation capacity and a slightly decreased cell proliferation rate but had similar STRO-1 expression, and exhibited a similar percentage of positive ex vivo osteogenic induction and dentin sialophosphoprotein expression from STRO-1-enriched pulp cells. Conclusion Our study provides preliminary evidence that clinically compromised dental pulp may contain putative cells with certain stem cell properties. Further characterization of these cells will provide insight regarding whether they could serve as a source of endogenous multipotent cells in tissue regeneration based dental pulp therapy. PMID:20416426

  20. Eggshells – assisted hydrolysis of banana pulp for biogas production

    African Journals Online (AJOL)

    KARAKANA

    In this study, pretreatment of banana pulp using eggshells in both calcined and un-calcined forms to examine the ... Key words: Anaerobic digestion, banana pulp hydrolysis biogas, eggshells. .... obtain fine powder. ..... using pig waste and cassava peels. ... from bioethanol waste: the effect of pH and urea addition to biogas.

  1. Antimicrobial activities of pomelo (Citrus maxima) seed and pulp ethanolic extract

    Science.gov (United States)

    Sahlan, Muhamad; Damayanti, Vina; Tristantini, Dewi; Hermansyah, Heri; Wijanarko, Anondho; Olivia, Yuko

    2018-02-01

    Grapefruit (Citrus paradisi) seed extract is generally used as naturopathic medications, supplements, antiseptic and disinfecting agents and also as preservatives in food and cosmetics products. In vitro studies have demonstrated that grapefruit seed extract has anti bacterial properties against a range of gram-positive and gram-negative organisms. Indonesian grapefruit, known as pomelo (C. maxima), has similar characteristics, contents and is under the same genus (Citrus) as grapefruit; however it has not been completely utilized as a preservative. In this work we analyze the antimicrobial activities of ethanolic extract of Indonesian pomelo (C. maxima) seeds and pulp compared to the grapefruit (C. paradisi) seeds and pulp ethanolic extract. Ethanolic extracts of pomelo and grapefruit seeds and pulp are investigated for activities against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Candida albicans. The level of antimicrobial effects is established using agar diffusion method. Both of the ethanolic do not show any antimicrobial activities against C. albicans. The ethanolic extract of pomelo seeds and pulp used in this research give positive results with growth inhibition effect on B. subtilis, S. aureus and E. coli. The zones of inhibition ranges from 22 - 30 mm in diameter, which is higher to grapefruit seeds and pulp ethanolic extract (17 - 25 mm). Ethanolic extract of pomelo seeds and pulp has an antimicrobial effect, which makes it a natural preparation for use as an alternative preservative for food and cosmetic.

  2. The dynamics of TGF-β in dental pulp, odontoblasts and dentin.

    Science.gov (United States)

    Niwa, Takahiko; Yamakoshi, Yasuo; Yamazaki, Hajime; Karakida, Takeo; Chiba, Risako; Hu, Jan C-C; Nagano, Takatoshi; Yamamoto, Ryuji; Simmer, James P; Margolis, Henry C; Gomi, Kazuhiro

    2018-03-13

    Transforming growth factor-beta (TGF-β) is critical for cell proliferation and differentiation in dental pulp. Here, we show the dynamic mechanisms of TGF-β in porcine dental pulp, odontoblasts and dentin. The mRNA of latent TGF-β1 and TGF-β3 is predominantly expressed in odontoblasts, whereas the mRNA expression level of latent TGF-β2 is high in dental pulp. TGF-β1 is a major isoform of TGF-β, and latent TGF-β1, synthesized in dental pulp, is primarily activated by matrix metalloproteinase 11 (MMP11). Activated TGF-β1 enhances the mRNA expression levels of MMP20 and full-length dentin sialophosphoprotein (DSPP) in dental pulp cells, coinciding with the induction of odontoblast differentiation. Latent TGF-β1 synthesized in odontoblasts is primarily activated by MMP2 and MMP20 in both odontoblasts and dentin. The activity level of TGF-β1 was reduced in the dentin of MMP20 null mice, although the amount of latent TGF-β1 expression did not change between wild-type and MMP20 null mice. TGF-β1 activity was reduced with the degradation of DSPP-derived proteins that occurs with ageing. We propose that to exert its multiple biological functions, TGF-β1 is involved in a complicated dynamic interaction with matrix metalloproteinases (MMPs) and/or DSPP-derived proteins present in dental pulp, odontoblasts and dentin.

  3. Physico-chemical, microbiological and sensory stability of chemically preserved mango pulp

    International Nuclear Information System (INIS)

    Akhtar, S.; Riaz, M.; Nisar, A.

    2010-01-01

    The effect of sodium benzoate (SB) and potassium metabisulphite (PMS) at various concentrations on chemical, microbiological and sensory quality of mango pulp during storage was assessed. Inhibitory activity of the chemical preservatives and their effect on chemical and sensory attributes was tested periodically by simulating the industrial mango pulp storage in the lab (30-42 deg. C in the dark), for a period of 90 days. Protein, fats, decreased while ash content and total soluble solid (TSS) increased during the storage period. A slight progressive decline in pH was observed with a proportional increase (p<0.05) in the acidity of the stored pulp samples. Significant inhibition of the total bacterial count (TBC) was observed on applying the specified concentrations, however PMS was shown to be more inhibitory. Storage time significantly (p<0.05) increased the CFU/g of the pulp samples as the maximum growth was observed after 90 days of storage. Sensory characteristics of the juice prepared from treated mango pulp samples were affected negatively on addition of preservatives however, the samples were accepted by the judges even after three months of storage. (author)

  4. Recycling cellulase towards industrial application of enzyme treatment on hardwood kraft-based dissolving pulp.

    Science.gov (United States)

    Wang, Qiang; Liu, Shanshan; Yang, Guihua; Chen, Jiachuan; Ji, Xingxiang; Ni, Yonghao

    2016-07-01

    Cost-effectiveness is vital for enzymatic treatment of dissolving pulp towards industrial application. The strategy of cellulase recycling with fresh cellulase addition was demonstrated in this work to activate the dissolving pulp, i.e. decreasing viscosity and increasing Fock reactivity. Results showed that 48.8-35.1% of cellulase activity can be recovered from the filtered liquor in five recycle rounds, which can be reused for enzymatic treatment of dissolving pulp. As a result, the recycling cellulase with addition fresh cellulase of 1mg/g led to the pulp of viscosity 470mL/g and Fock reactivity 80%, which is comparable with cellulase charge of 2mg/g. Other pulp properties such as alpha-cellulose, alkaline solubility and molecular weight distribution were also determined. Additionally, a zero-release of recycling cellulase treatment was proposed to integrate into the dissolving pulp production process. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Pectin methyl esterase activity in apple and orange pulps

    International Nuclear Information System (INIS)

    Abdullaev, A.; Djumaev, B.B.; Djumaev, N.B.; Mukhidinov, Z.K.

    2008-01-01

    The results of pectin methyl esterase activity from apple, orange pulp and orange peel depending of ph and temperature are discussed. It's shown that the methyl esterase activity form apple and orange pulps higher in range of temperatures from +37...+60 d ig C . The analysis of dependence of its activity from ph has shown that in both case the enzyme activity increase with increase of ph

  6. Process parameters affecting the delignification of eucalyptus kraft pulp with peroxyacetic acid

    Directory of Open Access Journals (Sweden)

    Chandranupap, P.

    2004-11-01

    Full Text Available Various process parameters affecting eucalyptus kraft pulp delignification with peroxyacetic acid were investigated. The results showed that pH was an important factor. The delignification rate increased with increasing pH to the value of 6. High delignification rate was obtained when the pulp was chelated with Na4-EDTA prior to the peroxyacetic acid stage. Therefore, delignification reaction rate depends on peroxyacid charge, temperature, pH and metal content of pulp.

  7. Electron treatment of wood pulp for the viscose process

    Science.gov (United States)

    Stepanik, T. M.; Ewing, D. E.; Whitehouse, R.

    2000-03-01

    Electron processing is currently being evaluated by several viscose producers for integration into their process. The viscose industry converts dissolving wood pulp into products such as staple fibre, filament, cord, film, packaging, and non-edible sausage casings. These materials are used in the clothing, drapery, hygiene, automobile, food, and packaging industries. Viscose producers are facing increasingly high production costs and stringent environmental regulations that have forced some plants to close. Electron treatment of wood pulp can significantly reduce the amounts of chemicals used for producing viscose and the production of hazardous pollutants. Acsion Industries has worked with companies worldwide to demonstrate the benefits of using electron treated pulp for producing viscose (rayon). This paper describes the viscose process, the benefits of using electron treatment in the viscose process, and Acsion's efforts in developing this technology.

  8. Biodegradation behaviors and color change of composites based on type of bagasse pulp/polylactic acid

    Directory of Open Access Journals (Sweden)

    maryam allahdadi

    2017-05-01

    Full Text Available In this research, appearance quality and decay resistance of polylactic acid (PLA based green composites made from monoethanolamine (MEA bagasse pulp, alkaline sulfite-anthraquinone (AS bagasse pulp, bleached soda (B S bagasse pulp, unbleached soda (UN S bagasse pulp (UN S bagasse pulp and raw bagasse fibers (B were investigated. For the investigation of biodegradation behaviors, effect of the white rot fungi (Coriolus versicolor on the neat PLA and composites with natural fibers during 30 and 60 days were studied. It is found that when the bagasse fibers were incorporated into composites matrix, percentage weight reduction and stiffness of samples have been increased. Also, the rate of loss mentioned of the composites made from bagasse pulp fibers were superior to the relevant raw bagase fibers. This can be explained by the removal of non-cellulosic components such as lignin and hemicelluloses from the fibers by pulping process. Also, the results indicates the inferior of surface qualities of fabricated composites regarding to neat PLA. Depending on the fiber type, different reductions of the surface qualities were attained. However, the degree of color change of the composites with any type of bagasse pulp fibers were lower compared with composite with raw bagasse fiber. Finally, as compared with the raw bagasse fibers, bagasse pulp fibers have better reinforcing capability.

  9. Histological evaluation of tissue reactions to newly synthetized calcium silicate- and hydroxyapatite-based bioactive materials: in vivo study

    Directory of Open Access Journals (Sweden)

    Opačić-Galić Vanja

    2017-01-01

    Full Text Available Introduction/Objective. Development of materials which could be used as biological bone substitutes is one of the most valuable and active fields of biomaterial research. The goal of the study was to research the reaction of tissue on calcium silicate- (CS and hydroxyapatitebased (CS-HA newly synthesized nanomaterials, after being implanted into the subcutaneous tissue of a rats and direct pulp capping of rabbit teeth. Methods. The tested materials were implanted in 40 Wistar male rats, sacrificed after seven, 15, 30, and 60 days. The direct pulp capping was performed on the teeth of rabbits. Cavities were prepared on the vestibular surface of the incisors. The animals were sacrificed after 10 and 15 days. The control material was mineral trioxide aggregate (MTA. Histological analysis covered the tracking of inflammatory reaction cellular components, presence of gigantic cells, and necrosis of the tissue. Results. Seven days after the implantation, the strongest inflammatory response was given by the MTA (3.3 Ѓ} 0.48, while CS and CS-HA scored 3 ± 0.71. After 60 days, the rate of inflammatory reactions dropped, which was the least visible with CS-HA (0.2 ± 0.45. The least visible inflammatory reaction of the rabbits’ pulp tissue was spotted with the CS (1.83 ± 0.75, than with the MTA and CS-HA (2.67 ± 1.53, 3 ± 0.63. Conclusion. The newly synthesized materials caused a slight reaction of the subcutaneous tissue. CS-HA showed the best tissue tolerance. Nanostructural biomaterials caused a slight to moderate inflammatory reaction of the rabbits’ pulp tissue only in the immediate vicinity of the implanted material.

  10. Assessing the value of pulp mill biomass savings in a climate change conscious economy

    International Nuclear Information System (INIS)

    Adahl, Anders; Harvey, Simon; Berntsson, Thore

    2006-01-01

    Pulp mills use significant amounts of biofuels, both internal and purchased. Biofuels could contribute to reach greenhouse gas emission targets at competitive costs. Implementing process integration measures at a pulp mill in order to achieve pulp production with less use of energy (biofuels) has not only on-site consequences but also off-site consequences, such as substitution of fossil fuels elsewhere by the saved pulp mill biofuels, and less on-site electric power generation. In this paper a method, a linking model, is suggested to analyse pulp mill biofuel saving measures when carbon dioxide (CO 2 ) external costs are internalised. The linking model is based on equilibrium economics and links information from CO 2 constrained energy market future scenarios with process integration measures. Pulp mill economics and marginal energy market CO 2 response are identified. In an applied study, four process integration measures at a Swedish pulp mill were analysed using five energy market future scenarios emanating from a Nordic energy model. The investigated investment alternatives for biofuel savings all result in positive net annual savings, irrespectively of the scenario used. However, CO 2 emissions may increase or decrease depending on the future development of the Nordic energy market

  11. The influence of irradiation of gamma-rays on the pulping and paper making, (3)

    International Nuclear Information System (INIS)

    Mori, Kenji; Sasaki, Toru; Hasegawa, Kunihiko.

    1979-01-01

    Dissolving pulp (DP) containing no lignin and cold soda pulp containing much amount of lignin were used for the study of the influence of gamma irradiation. Experiments were made in the presence of air, water, methanol, acetic anhydride, acetic anhydride + methanol, dioxane, dimethyl sulfoxide and 1% NaOH solution. Irradiation was made for 100 hours at 20 - 21 deg. C; total irradiation dose was 1.47 x 10 7 R. (1) In case of dimethyl sulfoxide, and especially in case of 1% NaOH solution, the yield decreased by irradiation, with cold soda pulp particularly. (2) In case of the pulp immersed in water, the brightness of pulp was not improved by irradiation, but in methanol, it was remarkably improved. Since the improvement was observed in both DP and cold soda pulps, it is caused by the action of oxidizing bleach with small amount of oxygen in the air remaining in the material, instead of the change in the quality of lignin. (3) By infrared analysis, methanol did not react with the lignin in cold soda pulp even under irradiation. (4) The acetylation was accelerated by irradiation. (J.P.N.)

  12. Fermentation of pulp from coffee production; Vergaerung von Pulpa aus der Kaffee-Produktion

    Energy Technology Data Exchange (ETDEWEB)

    Hofman, M.; Baier, U.

    2003-07-01

    Harvesting of coffee berries and production of dried coffee beans produces large amounts of solid wastes. Per ton of consumable coffee beans, roughly 2 tons of spent coffee pulp are wasted at the production facilities. Coffee pulp represents a valuable source of energy and can be used for anaerobic biogas production. In this study it was shown that coffee pulp can be anaerobically digested as a sole carbon source without further addition of co-substrates. No nutrient limitations and only a very moderate substrate inhibition have been found in concentrated pulp. The mesophilic biogas formation potential was found to be 0.38 m{sup 3} biogas per kg of organic matter. The anaerobic degradability was higher than 70%. In semi-continuously operated biogas reactors a high degradation of organics and a subsequent biogas production was shown at hydraulic detention times of 16 days. Methanization of fresh pulp is technically feasible in fully mixed tank reactors as well as in plug flow reactors. Due to the presence of easily degradable carbon sources, fresh pulp will quickly show microbiological growth. Storage in the presence of ambient oxygen will result in aerobic degradation of organics in parallel with energy loss. Additionally, anaerobic zones with methane emission will quickly occur. Therefore, it is recommended to store fresh pulp under oxygen free, lactic acid conditions (silage) until anaerobic treatment in the biogas reactor. (author)

  13. ATLAS electromagnetic end-cap detector

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    After the insertion of the first end-cap into this cryostat, the team proceed to the wiring operations. Millions of wires are connected to the electromagnetic calorimeter on this end-cap, whch must be carefully fed out from the detector so that data can be read out. The energy of photons, electrons and positrons will be measured as they pass through the end-cap having been created along the line of the beams in the proton-proton collisions.

  14. 21 CFR 872.1720 - Pulp tester.

    Science.gov (United States)

    2010-04-01

    ... battery powered device intended to evaluate the pulpal vitality of teeth by employing high frequency current transmitted by an electrode to stimulate the nerve tissue in the dental pulp. (b) Classification...

  15. ATLAS end-cap detector

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    Three scientists from the Institute of Nuclear Phyiscs at Novossibirsk with one of the end-caps of the ATLAS detector. The end-caps will be used to detect particles produced in the proton-proton collisions at the heart of the ATLAS experiment that are travelling close to the axis of the two beams.

  16. Preparation of lumen-loaded kenaf pulp with magnetite (Fe{sub 3}O{sub 4})

    Energy Technology Data Exchange (ETDEWEB)

    Zakaria, S.; Ong, B.H.; Ahmad, S.H.; Abdullah, M.; Yamauchi, T

    2005-02-15

    Magnetic pulps were prepared from unbleached kenaf (hibiscus cannabinus L.) kraft pulps. Fe{sub 3}O{sub 4} or magnetite powder was used to load into the pulp's lumen and pit. Aluminum sulphate [Al{sub 2}(SO{sub 4}){sub 3}] (alum) and polyethylenimine (PEI), both mainly function as retention aid were used throughout the experiment and found to be beneficial in the preparation of this magnetic pulps. The ash content method was used to determine the amount of magnetite retained in the lumen and pit. The utilization of PEI up to 2% per pulp fibres was found to be the best result on lumen loading. The deposition of magnetite powder in lumen and pit is found decrease as the addition of PEI used is more than 2% per pulp fibres. Scanning electron microscope (SEM) clearly shows the distribution of magnetite deposited in the lumen. Tensile index and folding endurance of the loaded fibre decreased slightly as the percentage of loading pigment increased.

  17. Characterization and sensory preference of fermented dairy beverages prepared with different concentrations of whey and araticum pulp

    Directory of Open Access Journals (Sweden)

    Alexsandra Valéria Sousa Costa de Lima

    2016-12-01

    Full Text Available The objective of this study was to develop a fermented dairy beverage flavored with araticum pulp, assess its physicochemical characteristics, microbiological quality, and sensory preference by the consumer. Araticum pulp was prepared using two different methods: with or without bleaching (50 ºC/5 minutes. Formulations of fermented dairy beverages consisting of whey (50%, standardized pasteurized milk (50%, and seven different concentrations of bleached araticum pulp (5.0, 7.5, 10.0, 12.5, 15.0, 17.5, and 20.0% w/v were prepared. In addition, seven formulations of fermented dairy beverage, without adding araticum pulp, and consisting of varying proportions of whey (40, 50, 60, 70, 80, 90, and 100% were developed. In all formulations, thickeners/stabilizers were added. All araticum pulp samples (with and without bleach and fermented dairy beverages (with and without araticum pulp were analyzed for the relevant physicochemical properties: pH, titratable acidity, acidity of pulp, acidity of fermented beverage, moisture, ash, fat, protein, crude fiber, ascorbic acid, carbohydrates, total solids, and caloric values. Microbiological counts of coliforms at 35 °C and 45 °C in the pulp and beverage, and molds and yeasts and Salmonella sp. in the pulp were obtained. Additionally, sensory analysis regarding preferences of the different fermented dairy beverage formulations was also performed. The araticum pulp samples without bleach, showed higher values of pH, moisture, protein, total fiber, and ascorbic acid, as compared to bleached pulp samples, while bleached araticum pulp showed higher values for other physicochemical parameters. Microbiological results showed that all pulps and fruit-dairy beverages were suitable for consumption. It was found that there was no significant consumer preference between different fermented beverage formulations, according to the different percentages of pulp. However, the formulations consisting of 40, 50, 60, and 70

  18. Determining the minimum conditions for soda-anthraquinone pulping of kenaf bast, core, and whole stalk fibers

    Science.gov (United States)

    James S. Han; Thomas A. Rymsza

    1999-01-01

    Chemical pulping of kenaf fiber is comparatively new. In this study, bast, core, and whole stalk kenaf fibers were pulped using a soda-AQ pulping process and various pulping conditions. Handsheets were evaluated for density, Canadian standard freeness, brightness, opacity, smoothness, and tensile, burst, and tear indexes and strength. The results indicate that...

  19. Molecular study of the dentin-pulp complex responses to caries progression

    Directory of Open Access Journals (Sweden)

    Yani Corvianindya Rahayu

    2007-03-01

    Full Text Available The dentin-pulp complex exhibits various responses to caries, including events of injury, defense, and repair. The overall responses dependent on pulp cell activity and the signaling processes, which regulate the behavior of these cells. The signals for tissue repair are thought to be mediated by dentin-bound growth factors released during caries progression. Growth factors are a key of molecules responsible for signaling a variety of cellular process following dental injury. The endogenous proteolytic enzymes (Matrix metalloproteinases, MMPs present in dentin matrix might also participate in releasing bioactive molecule. Several members of the MMP family are found in the soft and hard tissue compartment of dentin-pulp complex. Their presumed role in many physiological process during the development and maintenance of the dentin-pulp complex, they may also contribute to the pathogenesis of dentin caries and the responses elicited by caries.

  20. Mass balance of Mars' residual south polar cap from CTX images and other data

    Science.gov (United States)

    Thomas, P. C.; Calvin, W.; Cantor, B.; Haberle, R.; James, P. B.; Lee, S. W.

    2016-04-01

    Erosion of pits in the residual south polar cap (RSPC) of Mars concurrent with deposition and fluctuating cap boundaries raises questions about the mass balance and long term stability of the cap. Determining a mass balance by measurement of a net gain or loss of atmospheric CO2 by direct pressure measurements (Haberle, R.M. et al. [2014]. Secular climate change on Mars: An update using one Mars year of MSL pressure data. American Geophysical Union (Fall). Abstract 3947), although perhaps the most direct method, has so far given ambiguous results. Estimating volume changes from imaging data faces challenges, and has previously been attempted only in isolated areas of the cap. In this study we use 6 m/pixel Context Imager (CTX) data from Mars year 31 to map all the morphologic units of the RSPC, expand the measurement record of pit erosion rates, and use high resolution images to place limits on vertical changes in the surface of the residual cap. We find the mass balance in Mars years 9-31 to be -6 to +4 km3/♂y, or roughly -0.039% to +0.026% of the mean atmospheric CO2 mass/♂y. The indeterminate sign results chiefly from uncertainty in the amounts of deposition or erosion on the upper surfaces of deposits (as opposed to scarp retreat). Erosion and net deposition in this period appear to be controlled by summertime planetary scale dust events, the largest occurring in MY 9, another, smaller one in MY 28. The rates of erosion and the deposition observed since MY 9 appear to be consistent with the types of deposits and erosional behavior found in most of the residual cap. However, small areas (100 ♂y) of depositional and/or erosional conditions different from those occurring in the period since MY 9, although these environmental differences could be subtle.

  1. 21 CFR 884.5250 - Cervical cap.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Cervical cap. 884.5250 Section 884.5250 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES... cap. (a) Identification. A cervical cap is a flexible cuplike receptacle that fits over the cervix to...

  2. 21 CFR 888.3000 - Bone cap.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Bone cap. 888.3000 Section 888.3000 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES ORTHOPEDIC DEVICES Prosthetic Devices § 888.3000 Bone cap. (a) Identification. A bone cap is a mushroom...

  3. Effect of Cellulases and Xylanases on Refining Process and Kraft Pulp Properties.

    Directory of Open Access Journals (Sweden)

    Kamila Przybysz Buzała

    Full Text Available Samples of bleached kraft pine cellulosic pulp, either treated with an enzyme preparation (a Thermomyces lanuginosus xylanase, an Aspergillus sp. cellulase, and a multienzyme preparation NS-22086 containing both these activities or untreated, were refined in a laboratory PFI mill. The treatment with cellulases contained in the last two preparations significantly improved the pulp's susceptibility to refining (the target freeness value of 30°SR was achieved in a significantly shorter time, increased water retention value (WRV and fines contents while the weighted average fiber length was significantly reduced. These changes of pulp parameters caused deterioration of paper strength properties. The treatment with the xylanase, which partially hydrolyzed xylan, small amounts of which are associated with cellulose fibers, only slightly loosened the structure of fibers. These subtle changes positively affected the susceptibility of the pulp to refining (refining energy was significantly reduced and improved the static strength properties of paper. Thus, the treatment of kraft pulps with xylanases may lead to substantial savings of refining energy without negative effects on paper characteristics.

  4. Use of perborate in the bleaching of ethanolamine pulp from olive wood

    Energy Technology Data Exchange (ETDEWEB)

    Ramos, E.; Torre, M.J. de la; Gutiérrez, J.C.

    2016-11-01

    In this work, we studied the influence of the bleacher concentration(2.5-5.5%), temperature (60-80 ºC) and time (60-180 min) on the reagent (perborate) consumption by dry pulp, various properties of the bleached pulp (yield, kappa number, brightness and viscosity/kappa numberratio), and some physical properties of paper sheets obtainedfrom it (tear index, burst index, stretch and breakinglength). The pulp was previously obtained by ethanolamine-soda-anthraquinone cooking of olive wood. A face-centred composite factor design was used to derive equations relating the pulp properties to the operational variables with a view to identifying the optimum operating conditions. The equations thus obtained reproduced the experimental results with errors less than 10% in all cases.The most suitable operating conditions were found to be alow temperature (60 ºC), a long time (180 min) and a highperborate concentration (5,5%). Because the pulp brightness achieved never exceeded 63% -not even under themost drastic conditions-, the process should not be used with one-step bleaching sequences. (Author)

  5. Use of perborate in the bleaching of ethanolamine pulp from olive wood

    International Nuclear Information System (INIS)

    Ramos, E.; Torre, M.J. de la; Gutiérrez, J.C.

    2016-01-01

    In this work, we studied the influence of the bleacher concentration(2.5-5.5%), temperature (60-80 ºC) and time (60-180 min) on the reagent (perborate) consumption by dry pulp, various properties of the bleached pulp (yield, kappa number, brightness and viscosity/kappa numberratio), and some physical properties of paper sheets obtainedfrom it (tear index, burst index, stretch and breakinglength). The pulp was previously obtained by ethanolamine-soda-anthraquinone cooking of olive wood. A face-centred composite factor design was used to derive equations relating the pulp properties to the operational variables with a view to identifying the optimum operating conditions. The equations thus obtained reproduced the experimental results with errors less than 10% in all cases.The most suitable operating conditions were found to be alow temperature (60 ºC), a long time (180 min) and a highperborate concentration (5,5%). Because the pulp brightness achieved never exceeded 63% -not even under themost drastic conditions-, the process should not be used with one-step bleaching sequences. (Author)

  6. CENTRIFUGE END CAP

    Science.gov (United States)

    Beams, J.W.; Snoddy, L.B.

    1960-08-01

    An end cap for ultra-gas centrifuges is designed to impart or remove angular momentum to or from the gas and to bring the entering gas to the temperature of the gas inside the centrifuge. The end cap is provided with slots or fins for adjusting the temperature and the angular momentum of the entering gas to the temperature and momentum of the gas in the centrifuge and is constructed to introduce both the inner and the peripheral stream into the centrifuge.

  7. Imperative role of dental pulp stem cells in regenerative therapies: A systematic review

    Directory of Open Access Journals (Sweden)

    Ramchandra Kabir

    2014-01-01

    Full Text Available Stem cells are primitive cells that can differentiate and regenerate organs in different parts of the body such as heart, bones, muscles and nervous system. This has been a field of great clinical interest with immense possibilities of using the stem cells in regeneration of human organ those are damaged due to disease, developmental defects and accident. The knowledge of stem cell technology is increasing quickly in all medical specialties and in dental field too. Stem cells of dental origin appears to hold the key to various cell-based therapies in regenerative medicine, but most avenues are in experimental stages and many procedures are undergoing standardization and validation. Long-term preservation of SHED cells or DPSC is becoming a popular consideration, similar to the banking of umbilical cord blood. Dental pulp stem cells (DPSCs are the adult multipotent cells that reside in the cell rich zone of the dental pulp. The multipotent nature of these DPSCs may be utilized in both dental and medical applications. A systematic review of the literature was performed using various internet based search engines (PubMed, Medline Plus, Cochrane, Medknow, Ebsco, Science Direct, Hinari, WebMD, IndMed, Embase using keywords like "dental pulp stem cells", "regeneration", "medical applications", "tissue engineering". DPSCs appears to be a promising innovation for the re-growth of tissues however, long term clinical studies need to be carried out that could establish some authentic guidelines in this perspective.

  8. Imperative role of dental pulp stem cells in regenerative therapies: a systematic review.

    Science.gov (United States)

    Kabir, Ramchandra; Gupta, Manish; Aggarwal, Avanti; Sharma, Deepak; Sarin, Anurag; Kola, Mohammed Zaheer

    2014-01-01

    Stem cells are primitive cells that can differentiate and regenerate organs in different parts of the body such as heart, bones, muscles and nervous system. This has been a field of great clinical interest with immense possibilities of using the stem cells in regeneration of human organ those are damaged due to disease, developmental defects and accident. The knowledge of stem cell technology is increasing quickly in all medical specialties and in dental field too. Stem cells of dental origin appears to hold the key to various cell-based therapies in regenerative medicine, but most avenues are in experimental stages and many procedures are undergoing standardization and validation. Long-term preservation of SHED cells or DPSC is becoming a popular consideration, similar to the banking of umbilical cord blood. Dental pulp stem cells (DPSCs) are the adult multipotent cells that reside in the cell rich zone of the dental pulp. The multipotent nature of these DPSCs may be utilized in both dental and medical applications. A systematic review of the literature was performed using various internet based search engines (PubMed, Medline Plus, Cochrane, Medknow, Ebsco, Science Direct, Hinari, WebMD, IndMed, Embase) using keywords like "dental pulp stem cells", "regeneration", "medical applications", "tissue engineering". DPSCs appears to be a promising innovation for the re-growth of tissues however, long term clinical studies need to be carried out that could establish some authentic guidelines in this perspective.

  9. Breeding Strategy To Generate Robust Yeast Starter Cultures for Cocoa Pulp Fermentations

    Science.gov (United States)

    Meersman, Esther; Steensels, Jan; Paulus, Tinneke; Struyf, Nore; Saels, Veerle; Mathawan, Melissa; Koffi, Jean; Vrancken, Gino

    2015-01-01

    Cocoa pulp fermentation is a spontaneous process during which the natural microbiota present at cocoa farms is allowed to ferment the pulp surrounding cocoa beans. Because such spontaneous fermentations are inconsistent and contribute to product variability, there is growing interest in a microbial starter culture that could be used to inoculate cocoa pulp fermentations. Previous studies have revealed that many different fungi are recovered from different batches of spontaneous cocoa pulp fermentations, whereas the variation in the prokaryotic microbiome is much more limited. In this study, therefore, we aimed to develop a suitable yeast starter culture that is able to outcompete wild contaminants and consistently produce high-quality chocolate. Starting from specifically selected Saccharomyces cerevisiae strains, we developed robust hybrids with characteristics that allow them to efficiently ferment cocoa pulp, including improved temperature tolerance and fermentation capacity. We conducted several laboratory and field trials to show that these new hybrids often outperform their parental strains and are able to dominate spontaneous pilot scale fermentations, which results in much more consistent microbial profiles. Moreover, analysis of the resulting chocolate showed that some of the cocoa batches that were fermented with specific starter cultures yielded superior chocolate. Taken together, these results describe the development of robust yeast starter cultures for cocoa pulp fermentations that can contribute to improving the consistency and quality of commercial chocolate production. PMID:26150457

  10. Hydrogen production from carrot pulp by the extreme thermophiles Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana

    Energy Technology Data Exchange (ETDEWEB)

    Vrije, Truus de; Budde, Miriam A.W.; Lips, Steef J.; Bakker, Robert R.; Mars, Astrid E.; Claassen, Pieternel A.M. [Wageningen UR, Food and Biobased Research, P.O. Box 17, 6700 AA Wageningen (Netherlands)

    2010-12-15

    Hydrogen was produced from carrot pulp hydrolysate, untreated carrot pulp and (mixtures of) glucose and fructose by the extreme thermophiles Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana in pH-controlled bioreactors. Carrot pulp hydrolysate was obtained after enzymatic hydrolysis of the polysaccharide fraction in carrot pulp. The main sugars in the hydrolysate were glucose, fructose, and sucrose. In fermentations with glucose hydrogen yields and productivities were similar for both strains. With fructose the hydrogen yield of C. saccharolyticus was reduced which might be related to uptake of glucose and fructose by different types of transport systems. With T. neapolitana the fructose consumption rate and consequently the hydrogen productivity were low. The hydrogen yields of both thermophiles were 2.7-2.8 mol H{sub 2}/mol hexose with 10 g/L sugars from carrot pulp hydrolysate. With 20 g/L sugars the yield of T. neapolitana was 2.4 mol H{sub 2}/mol hexose while the yield of C. saccharolyticus was reduced to 1.3 mol H{sub 2}/mol hexose due to high lactate production in the stationary growth phase. C. saccharolyticus was able to grow on carrot pulp and utilized soluble sugars and, after adaptation, pectin and some (hemi)cellulose. No growth was observed with T. neapolitana when using carrot pulp in agitated fermentations. Enzymatic hydrolysis of the polysaccharide fraction prior to fermentation increased the hydrogen yield with almost 10% to 2.3 g/kg of hydrolyzed carrot pulp. (author)

  11. PENGGUNAAN ASAM PERASETAT PADA PROSES DELIGNIFIKASI PULP ACETOSOLV DARI AMPAS TEBU DAN BAMBU BETUNG

    Directory of Open Access Journals (Sweden)

    Ahmad Sapta Zuidar

    2014-07-01

    Full Text Available Acetosolv process produced dark pulp because of the high lignin content.  Therefore ,  delignification  process is needed. This process uses peracetic acid to improve pulp qualities. The objective of this research was to determine the effect of different concentrations of peracetic acid against the characteristics of pulp acetosolv from bagasse and bamboo betung.  The research was arranged in a Complete Randomized Block Design with a single treatment and 4 replications.  The treatment used peracetic acid with six levels concentration (9%, 11%, 13%, 15%, 17%, 19% at a temperature of 85oC for 3 hours. The homogenity and additivity of the data were  analyzed using Bartlett and Tuckey Tests, then they were analyze for ANOVA to see if there is any difference among means, and then processed further using  Least Significant Difference at level of 1% and 5%.  The results showed that the concentration of peracetic acid had significant effect on yield, cellulose, hemicellulose, lignin, and organoleptic color pulp from bagasse and bamboo betung.  The best results showed that the concentration of peracetic acid 15% with cooking duration of  3 hours gave the best pulp. The pulp contained 85.837% of cellulose , 7.757% of hemicellulose , 1.758% of lignin , 73.048% of yield  and  the average organoleptic score for the pulp color was 4.3. Keywords:   acetosolv pulp,  baggase, bamboo,  delignification, paracetic acid

  12. THE TURN OF THE MONTH EFFECT CONTINUED: A COMPARISON OF SMALL CAP STOCKS AND LARGE CAP STOCKS

    OpenAIRE

    Ramsundhar, Shamman

    2010-01-01

    The purpose of this paper is to investigate whether the turn of the month effect occurs in small cap and large cap stocks and if it occurs in both categories, to determine whether there is a difference in the magnitude. My research, for the period of 1963-2008, based on the CRSP value weighted index, shows that there is a significant turn of the month effect in small and large cap stocks, however the effect is larger in small cap stocks. Furthermore, this effect is not limited to a short time...

  13. Statistically designed optimisation of enzyme catalysed starch removal from potato pulp

    DEFF Research Database (Denmark)

    Thomassen, Lise Vestergaard; Meyer, Anne S.

    2010-01-01

    to obtain dietary fibers is usually accomplished via a three step, sequential enzymatic treatment procedure using a heat stable alpha-amylase, protease, and amyloglucosidase. Statistically designed experiments were performed to investigate the influence of enzyme dose, amount of dry matter, incubation time...... and temperature on the amount of starch released from the potato pulp. The data demonstrated that all the starch could be released from potato pulp in one step when 8% (w/w) dry potato pulp was treated with 0.2% (v/w) (enzyme/substrate (E/S)) of a thermostable Bacillus licheniformis alpha-amylase (Termamyl(R) SC...

  14. Papaya pulp gelling: is it premature ripening or problems of water accumulation in the apoplast?

    Directory of Open Access Journals (Sweden)

    Jurandi Gonçalves de Oliveira

    Full Text Available Gelled aspect in papaya fruit is typically confused with premature ripening. This research reports the characterization of this physiological disorder in the pulp of papaya fruit by measuring electrolyte leakage, Pi content, lipid peroxidation, pulp firmness, mineral contents (Ca, Mg and K - in pulp and seed tissues, and histological analysis of pulp tissue. The results showed that the gelled aspect of the papaya fruit pulp is not associated with tissue premature ripening. Data indicate a reduction of the vacuole water intake as the principal cause of the loss of cellular turgor; while the waterlogged aspect of the tissue may be due to water accumulation in the apoplast.

  15. Pulp-mill related polychlorinated organic compounds in Baltic Sea sediments

    Energy Technology Data Exchange (ETDEWEB)

    Jonsson, Per (Swedish Environmental Protection Agency, Solna (Sweden)); Rappe, C.; Kjeller, L.O. (Umeaa Univ. (Sweden). Inst. of Environmental Chemistry); Kierkegaard, A. (Stockholm Univ. (Sweden). Inst. of Applied Environmental Research); Haakansson, L. (Uppsala Univ. (Sweden). Inst. of Earth Sciences); Jonsson, Bjoern (County Adm. Board, Umeaa (Sweden))

    1993-01-01

    Sediment samples from the Baltic basin were analyzed for PCDD/Fs, alkyl-CDFs, chloroguaiacols and EOCl. The EOCl distribution pattern in the Iggesund pulp-mill area shows high sediment concentrations close to the mill, and decreasing concentrations with increasing distance from the mill. Two series of alkyl-CDFs have been identified. The PCDD/Fs in offshore surficial sediments from the Baltic proper show a typical incineration pattern, while sediments collected in the vicinity of the mill show enhanced levels of 2,3,7,8-tetraCDD, 2,3,7,8- and 1,2,7,8-tetraCDF, and 1,2,3,7,8-pentaCDD. These PCDD/F congeners are statistically correlated to di-, tri-, and tetrachloroguaiacols, alkyl-CDFs, EOCl, and to the distance from the pulp mill, indicating the same source and transportation regime. Principal component analysis of the data set supports this conclusion. The three 2,3,7,8-tetrachloro-substituted PCDD/F congeners have formerly been found in high concentrations in sediments and crab hepatopancreas close to a pulp mill on the Swedish west coast. Our results from a pulp mill on the Swedish east coast support the conclusion that these congeners are typically associated with pulp bleaching. Deep layer sediments have significantly lower levels of PCDD/Fs. The isomer profiles are different in the surficial and the deep sediments, indicating a more natural preindustrial production of PCDD/Fs. (53 refs., 7 figs.).

  16. Effect of tomato post-harvest fungicide treatment and storage conditions on the quality of fruits, and biological value of tomato pulp and concentrated pulp

    Directory of Open Access Journals (Sweden)

    H. Parynow

    2013-12-01

    Full Text Available The influence of storage conditions on the quality of tomato fruits was tested. The rate of ripening was established in normal air, where tomatoes ripen quickly, under controlled atmosphere where they ripen more slowly and under low pressure, where they ripen slowest. The influence of post-harvest benomyl or methylthiophanate treatment on tomato rot, ripening, and biological value were examined. Post-harvest tomato treatment did not reduce fruit rot. The color of fruits and the processed products depended on the fungicide treatment. Concentrated tomato pulp made of fruits treated with methylthiophanate was redder than the others. The fungicide treatment increased or decreased the level of some chemical substances in the fruits in dependence on the applied fungicide, storage conditions and the length of storage, e.g. tomatoes treated with benomyl and stored for 14 days contained the highest level of vitamin C under 0% CO2:3%O2 and tomatoes treated with methylthiophanate contained the highest level of vitamin C under 38 mm Hg. Degradation of vitamin C in pulp was faster than in the concentrated pulp. Tomato pulp made of tomatoes treated with methylthiophanate contained the lowest level of vitamin C.

  17. NLRP3 inflammasome inhibition is disrupted in a group of auto-inflammatory disease CAPS mutations.

    Science.gov (United States)

    Mortimer, Leanne; Moreau, France; MacDonald, Justin A; Chadee, Kris

    2016-10-01

    Inflammasomes are positioned to rapidly escalate the intensity of inflammation by activating interleukin (IL)-1β, IL-18 and cell death by pyroptosis. However, negative regulation of inflammasomes remains poorly understood, as is the signaling cascade that dampens inflammasome activity. We found that rapid NLRP3 inflammasome activation was directly inhibited by protein kinase A (PKA), which was induced by prostaglandin E2 (PGE2) signaling via the PGE2 receptor E-prostanoid 4 (EP4). PKA directly phosphorylated the cytoplasmic receptor NLRP3 and attenuated its ATPase function. We found that Ser295 in human NLRP3 was critical for rapid inhibition and PKA phosphorylation. Mutations in NLRP3-encoding residues adjacent to Ser295 have been linked to the inflammatory disease CAPS (cryopyrin-associated periodic syndromes). NLRP3-S295A phenocopied the human CAPS mutants. These data suggest that negative regulation at Ser295 is critical for restraining the NLRP3 inflammasome and identify a molecular basis for CAPS-associated NLRP3 mutations.

  18. The influence of irradiation of gamma-rays on the pulping and paper making, (4)

    International Nuclear Information System (INIS)

    Suzuki, Kyoji; Inoue, Kaoru; Hanamura, Norio; Mori, Kenji

    1980-01-01

    The influence of gamma-irradiation on the beating properties of unbleached kraft pulps was studied, and the changes of the mechanical and chemical properties of the sheet made from those pulps were also investigated. The results obtained were as follows: (1) When the unbeaten pulp was treated with gamma-ray, the degree of polymerization of cellulose was decreased rapidly and the formation of aldehyde and carboxyl groups in pulp was observed in addition to that the beating time of irradiated pulps was reduced comparing with non-irradiated pulp. These effects increased roughly in proportion to the radiation dose. (2) Gamma-irradiation was more effective in wet state (moisture content = 70 - 80%) than air dry state. This may be due to the degradation products of water by gamma-irradiation. (3) The mechanical properties (breaking length, tear and burst factors) of the sheets made from irradiated pulps were considerably deteriorated at 10 7 R, but there was a slight deterioration up to 10 6 R. (4) Comparing the result of the mechanical properties, the strengths of the various sheets were shown in the following order: the sheet irradiated after paper making gt the sheet irradiated before beating (air dry state) gt the sheet irradiated before beating (wet state). (author)

  19. Histological evaluation of the pulp in teeth from dogs with naturally occurring periodontal disease.

    Science.gov (United States)

    Nemec, Ana; Pavlica, Zlatko; Stiblar-Martincic, Draga; Petelin, Milan; Erzen, Damjan; Crossley, David

    2007-12-01

    The purpose of this investigation was to evaluate the pulp of dog teeth affected by advanced periodontal disease. Histological examination was done on demineralized teeth extracted during clinical treatment of mature, client owned small and medium-size breed dogs with either good periodontal health or with advanced naturally occurring periodontal disease. Routinely stained sections from 5 clinically normal teeth and 22 teeth with advanced periodontitis from dogs between 5 and 12-years of age were examined using light microscopy. The pulp cavities of most teeth were narrow with low cellularity and some fibrosis of the pulp. Findings specific to periodontally affected teeth included acute and chronic pulpitis, vascular congestion, and pulp necrosis. A glomus body was identified in the pulp of one tooth and areas of poorly mineralized cementum were seen in both normal and diseased teeth. Age related changes in dog teeth appear similar to those reported for man and the rat. In addition to age related changes, the pulp of dog teeth with advanced periodontal disease were frequently inflamed or necrotic. This may reflect the advanced periodontitis affecting these teeth or a mechanical effect related to excessive tooth mobility. Further study is required to determine the etiology and significance of these findings and to investigate pulp status in less severely diseased teeth.

  20. Effects of hot water pre-extraction on surface properties of bagasse soda pulp.

    Science.gov (United States)

    Cordeiro, Nereida; Ashori, Alireza; Hamzeh, Yahya; Faria, Marisa

    2013-03-01

    In this work, the effects of hot water pre-extraction of depithed bagasse on the soda pulping and surface properties were studied. The conditions of hot water pre-extraction were: maximum temperature 170 °C, heat-up time 90 min, time at maximum temperature 10 min, and solid to liquor ratio (S:L) 1:8. Consequently, the pre-extracted and un-extracted bagasse chips were subjected to soda pulping at 160 °C for 1h with 11, 14 and 17% active alkali charge and an S:L of 1:5. The results showed that the hot water pre-extraction increased bagasse surface texture porosity by hemicellulose degradation. Therefore, the delignification was faster for pulping of pre-extracted samples. At a certain charge of alkali, pre-extracted samples showed higher screened yield and lower Kappa number. For instance, at 17% alkali charge, pre-extracted bagasse gave 11.3% higher pulp yield compared with the un-extracted ones. Inverse gas chromatography (IGC) results showed that the hot water pre-extraction changed the active sites on the bagasse surface, decreasing the dispersive energy and the basicity character, and affected the particle morphology. The pulping process decreased the hydrophobicity and the basicity of the bagasse surface. The surfaces of un-extracted and pre-extracted bagasse pulps had similar properties but different morphology. The pulps present higher surface area and permeability with more reactive capacity. Copyright © 2012 Elsevier B.V. All rights reserved.

  1. Prevalence of Coronal Pulp Stones and Its Relation with Systemic Disorders in Northern Indian Central Punjabi Population

    Science.gov (United States)

    Bains, Sandeep Kumar; Bhatia, Archana; Singh, Harkanwal Preet; Biswal, Swati Swagatika; Kanth, Shashi; Nalla, Srinivas

    2014-01-01

    Aim. To estimate the prevalence of coronal pulp stones in the molar teeth of dental outpatients of Sunam, Sangrur district, Punjab, India, to report any association between occurrence of pulp stones with age, gender, dental arch, side, and dental status and to find out correlation between pulp stones with dental and systemic diseases. Materials and Methods. 500 routine dental outpatients within age group of 18–67 years were involved in the study. Molar bitewing of left and right side of each patient was taken with XCP bitewing instrument and size 2 film. The presence or absence of pulp stones was recorded. Chi-square analysis was used to record the prevalence of pulp stones and to compare it with demographic and systemic factors. Results. Overall prevalence of pulp stones was 41.8%. Pulp stones were significantly higher in maxilla (11.59%) than mandible (6.54%), left side than right side, and first molar than other molars. Higher numbers of pulp stones were recorded in patients with cardiovascular disease (38.89%) than with cholelithiasis and renal lithiasis. Conclusion. Pulp stones were higher in maxillary arch than mandibular arch and in females than males. Cardiovascular patients had higher number of pulp stones than other groups. PMID:24944821

  2. RELATIONSHIP BETWEEN CRYSTALLINE STRUCTURE AND OPTICAL PROPERTIES OF WHEAT (Triticum aestevum L. STRAW SODA-OXYGEN PULP

    Directory of Open Access Journals (Sweden)

    Esat Gümüşkaya

    2003-04-01

    Full Text Available In this study; pulp was produced with soda-oxygen process by using wheat (Triticum aestevum L. straw as raw material and this pulp bleached with hypocholoride (H and peroxyde (P stages. It was found that crystalline properties of unbleached and bleached pulp samples increased by removing amorphous components. In addition, paper sheets made from unbleached and bleached soda-oxygen pulp and determined their optical properties. Consequently; while crystalline properties of pulp samples was rising with HP bleaching, it was determined that optical properties of paper sheets improved with bleaching.

  3. Carcass characteristics and meat quality of heavy swine fed different citrus pulp levels

    Directory of Open Access Journals (Sweden)

    P.H. Watanabe

    2010-08-01

    Full Text Available An assay with 36 swine initially weighting 83.7±5.1kg body weight (BW was carried out to evaluate the effects of the use of different dietary citrus pulp levels, 0, 10%, 20%, and 30%, upon digestive organs weights, carcass characteristics, and meat quality of animals subjected to qualitative feed restriction program, and slaughtered at 130kg BW. Linear response (P0.05. Higher levels of citrus pulp neither decreased backfat thickness nor increased amount of lean meat, indicative that qualitative feed restriction was not efficient. Positive linear effect (P<0.05 on pH measured 24 hours after slaughter and negative linear effect (P<0.05 on color characteristics as function of citrus pulp dietary levels were verified. Citrus pulp addition in qualitative feed restriction program may not be effective. As no deleterious effects upon meat qualities were observed, citrus pulp can be used as an alternative feedstuff for finishing swine.

  4. Yield-increasing additives in kraft pulping: Effect on carbohydrate retention, composition and handsheet properties

    Energy Technology Data Exchange (ETDEWEB)

    Vaaler, David Andre Grimsoeen

    2008-07-01

    In this thesis, increased hemicellulose retention during kraft pulping has been studied. The work has been divided into three parts: i) Development of an accessible and reliable method for determination of carbohydrate composition of kraft pulps ii) Investigation of the composition and molecular mass distributions of the carbohydrates in kraft pulps with increased hemicellulose content iii) Investigation of the effect of increased hemicellulose content on the sheet properties of kraft pulps with increased hemicellulose content. A method for carbohydrate determination was developed. In this method, enzymes are used to hydrolyse the pulp into monosaccharides. A relatively mild acid hydrolysis is performed prior to detection on an HPLC with an RI-detector. The pulp is not derivatized and no pre-treatment (mechanical or chemical) is needed to determine the carbohydrate composition using the method developed here. Peak deconvolution software is used to improve the accuracy. Polysulphide and H2S primarily increase the glucomannan yield, which can be boosted by up to 7 % on o.d. wood. However, the cellulose yield is more affected by the cooking time and the maximum yield increase of cellulose is approximately 2 % on o.d. wood compared to an ordinary kraft pulp. The cooking time is influenced by sulphide ion concentration, AQ addition and the final Kappa number. The xylan yield is remarkably stable, however the alkali profile during the cook may influence the xylan yield. Surface xylan content of the fibres depends on residual alkali concentration in the black liquor. The molecular mass distributions of cellulose and hemicellulose were determined for pulps with increased hemicellulose content using size exclusion chromatography. Deconvolution by peak separation software is used to gain information about the degree of polymerization for cellulose and hemicellulose. The average DP of glucomannan in the kraft fibre was found to be 350 +- 30 and the average DP of xylan in the

  5. PDGFRβ+/c-kit+ pulp cells are odontoblastic progenitors capable of producing dentin-like structure in vitro and in vivo.

    Science.gov (United States)

    Cai, Shiwei; Zhang, Wenjian; Chen, Wei

    2016-10-28

    Successful pulp regeneration depends on identification of pulp stem cells capable of differentiation under odontoblastic lineage and producing pulp-dentinal like structure. Recent studies demonstrate that platelet-derived growth factor (PDGF) plays an important role in damage repair and tissue regeneration. The aim of this study was to identify a subpopulation of dental pulp cells responsive to PDGF and with dentin regeneration potential. Pulp tissues were isolated from 12 freshly extracted human impacted third molars. Pulp cells were sorted by their expression of PDGFRβ and stem cell marker genes via flow cytometry. For the selected cells, proliferation was analyzed by a colorimetric cell proliferation assay, differentiation was assessed by real time PCR detection the expression of odontoblast marker genes, and mineralization was evaluated by Alizarin Red S staining. GFP marked PDGFRβ + /c-kit + pulp cells were transplanted into emptied root canals of nude rat lower left incisors. Pulp-dentinal regeneration was examined by immunohistochemistry. PDGFRβ + /c-kit + pulp cells proliferated significantly faster than whole pulp cells. In mineralization media, PDGFRβ + /c-kit + pulp cells were able to develop under odontoblastic linage as demonstrated by a progressively increased expression of DMP1, DSPP, and osteocalcin. BMP2 seemed to enhance whereas PDGF-BB seemed to inhibit odontoblastic differentiation and mineralization of PDGFRβ + /c-kit + pulp cells. In vivo root canal transplantation study revealed globular dentin and pulp-like tissue formation by PDGFRβ + /c-kit + cells. PDGFRβ + /c-kit + pulp cells appear to have pulp stem cell potential capable of producing dentinal like structure in vitro and in vivo.

  6. Quality evaluation of frozen guava and yellow passion fruit pulps by NIR spectroscopy and chemometrics.

    Science.gov (United States)

    Alamar, Priscila D; Caramês, Elem T S; Poppi, Ronei J; Pallone, Juliana A L

    2016-07-01

    The present study investigated the application of near infrared spectroscopy as a green, quick, and efficient alternative to analytical methods currently used to evaluate the quality (moisture, total sugars, acidity, soluble solids, pH and ascorbic acid) of frozen guava and passion fruit pulps. Fifty samples were analyzed by near infrared spectroscopy (NIR) and reference methods. Partial least square regression (PLSR) was used to develop calibration models to relate the NIR spectra and the reference values. Reference methods indicated adulteration by water addition in 58% of guava pulp samples and 44% of yellow passion fruit pulp samples. The PLS models produced lower values of root mean squares error of calibration (RMSEC), root mean squares error of prediction (RMSEP), and coefficient of determination above 0.7. Moisture and total sugars presented the best calibration models (RMSEP of 0.240 and 0.269, respectively, for guava pulp; RMSEP of 0.401 and 0.413, respectively, for passion fruit pulp) which enables the application of these models to determine adulteration in guava and yellow passion fruit pulp by water or sugar addition. The models constructed for calibration of quality parameters of frozen fruit pulps in this study indicate that NIR spectroscopy coupled with the multivariate calibration technique could be applied to determine the quality of guava and yellow passion fruit pulp. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Pulp fictions of medieval England: Essays in popular romance

    OpenAIRE

    McDonald, Nicola

    2004-01-01

    Middle English popular romance is the most audacious and compendious testimony to the imaginary world of the English Middle Ages. Yet, with few exceptions, it remains under read and under studied. Pulp fictions of medieval England demonstrates that popular romance merits and rewards serious critical attention and that it is crucial to our understanding of the complex and conflicted world of medieval England. Pulp fictions of medieval England comprises ten essays on individual romances that, w...

  8. Regenerative medicine in dental and oral tissues: Dental pulp mesenchymal stem cell

    Directory of Open Access Journals (Sweden)

    Janti Sudiono

    2017-08-01

    Full Text Available Background. Regenerative medicine is a new therapeutic modality using cell, stem cell and tissue engineering technologies. Purpose. To describe the regenerative capacity of dental pulp mesenchymal stem cell. Review. In dentistry, stem cell and tissue engineering technologies develop incredibly and attract great interest, due to the capacity to facilitate innovation in dental material and regeneration of dental and oral tissues. Mesenchymal stem cells derived from dental pulp, periodontal ligament and dental follicle, can be isolated, cultured and differentiated into various cells, so that can be useful for regeneration of dental, nerves, periodontal and bone tissues. Tissue engineering is a technology in reconstructive biology, which utilizes mechanical, cellular, or biological mediators to facilitate regeneration or reconstruction of a particular tissue. The multipotency, high proliferation rates and accessibility, make dental pulp as an attractive source of mesenchymal stem cells for tissue regeneration. Revitalized dental pulp and continued root development is the focus of regenerative endodontic while biological techniques that can restore lost alveolar bone, periodontal ligament, and root cementum is the focus of regenerative periodontic. Conclucion. Dentin-derived morphogens such as BMP are known to be involved in the regulation of odontogenesis. The multipotency and angiogenic capacity of DPSCs as the regenerative capacity of human dentin / pulp complex indicated that dental pulp may contain progenitors that are responsible for dentin repair. The human periodontal ligament is a viable alternative source for possible primitive precursors to be used in stem cell therapy.

  9. Breeding Strategy To Generate Robust Yeast Starter Cultures for Cocoa Pulp Fermentations.

    Science.gov (United States)

    Meersman, Esther; Steensels, Jan; Paulus, Tinneke; Struyf, Nore; Saels, Veerle; Mathawan, Melissa; Koffi, Jean; Vrancken, Gino; Verstrepen, Kevin J

    2015-09-01

    Cocoa pulp fermentation is a spontaneous process during which the natural microbiota present at cocoa farms is allowed to ferment the pulp surrounding cocoa beans. Because such spontaneous fermentations are inconsistent and contribute to product variability, there is growing interest in a microbial starter culture that could be used to inoculate cocoa pulp fermentations. Previous studies have revealed that many different fungi are recovered from different batches of spontaneous cocoa pulp fermentations, whereas the variation in the prokaryotic microbiome is much more limited. In this study, therefore, we aimed to develop a suitable yeast starter culture that is able to outcompete wild contaminants and consistently produce high-quality chocolate. Starting from specifically selected Saccharomyces cerevisiae strains, we developed robust hybrids with characteristics that allow them to efficiently ferment cocoa pulp, including improved temperature tolerance and fermentation capacity. We conducted several laboratory and field trials to show that these new hybrids often outperform their parental strains and are able to dominate spontaneous pilot scale fermentations, which results in much more consistent microbial profiles. Moreover, analysis of the resulting chocolate showed that some of the cocoa batches that were fermented with specific starter cultures yielded superior chocolate. Taken together, these results describe the development of robust yeast starter cultures for cocoa pulp fermentations that can contribute to improving the consistency and quality of commercial chocolate production. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  10. Review on Bamboo Utilization as Biocomposites, Pulp and Bioenergy

    Science.gov (United States)

    Yusuf, Sulaeman; Syamani, F. A.; Fatriasari, W.; Subyakto

    2018-03-01

    One of potential non wood bioresources utilized in industrial application is bamboos. Bamboos are include in graminae family which have high biomass productivity, easy and rapid production, wide avability and high holocellulose content. Indonesia has a huge potential of bamboos, more than 162 bamboo species are found however only some of them are planted that have a high economic value. Bamboos have some advantages such as can be harvested at 3 years, straight culm, high strength, easy to be processed, and relatively cheap. Research Center for Biomaterials has developed utilization of bamboo culm for ply bamboo product as alternative of plywood since 1995, using gombong bamboo, tali bamboo, sembilang bamboo, andong bamboo with PF resin as adhesive. Other biocomposite products from bamboos include particle board, cement board and polymer-bamboo fiber composites. In term of processing technique and final product quality, bamboo composites from ply bamboo are the most prospectable material to be utilized in industrial application. Yellow bamboo and betung bamboo have also been developed as pulp and paper. Biopulping using soda and kraft pulping after biological pretreatment using white rot fungi to remove lignin was used as pulping method in this conversion. Biokraft pulping with Trametes versicolor for 45 days with inoculum loading of 10% resulted better pulp quality compared to the other fungi. Betung bamboo had good morphological characteristics and chemical component content to be converted into bioenergy such as bioethanol. Several pretreatment methods have been developed in order to result high sugar yield. Microwave assisted acid hydrolysis was preferedin producing higher yield from the pretreated bamboo compared to enzymatic hydrolysis. By using this method, the bamboo pretreated by biological-microwave pretreatment results higher improvement to increase sugar yield.

  11. Electrochemical deposition mechanism of calcium phosphate coating in dilute Ca-P electrolyte system

    Energy Technology Data Exchange (ETDEWEB)

    Hu Ren [State Key Laboratory of Physical Chemistry of Solid Surfaces and Department of Chemistry, CNRS Laboratoire International Associe XiamENS, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005 (China); Department of Biology, College of Life Science, Xiamen University, Xiamen, Fujian 361005 (China); Lin Changjian, E-mail: cjlin@xmu.edu.cn [State Key Laboratory of Physical Chemistry of Solid Surfaces and Department of Chemistry, CNRS Laboratoire International Associe XiamENS, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005 (China); Shi Haiyan; Wang Hui [State Key Laboratory of Physical Chemistry of Solid Surfaces and Department of Chemistry, CNRS Laboratoire International Associe XiamENS, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005 (China)

    2009-06-15

    In this work, the electrochemical deposition behavior of calcium phosphate coating from an aqueous electrolyte containing very dilute calcium and phosphorus species (Ca-P) was studied. The effects of three process parameters, i.e. temperature, current density and duration, were systematically investigated and the underlying mechanism was thoroughly analyzed. It was observed that the coating is mainly composed of hydroxyapatite (HA) in a wide range of temperature and current densities. The temperature had a significant effect on the deposition velocity. An apparent activation energy of 174.9 kJ mol{sup -1} was subsequently derived, indicating the mass-transfer control mechanism for the coating formation. The current density was identified to be an important parameter for structure controllability. The results of DR-FTIR/Raman spectroscopic studies of the initial deposition phase strongly suggested that the HA coating was instantaneously and directly precipitated on the substrate; neither induction period nor precursor was detected in this dilute Ca-P electrolyte system. Finally, a phase diagram of the Ca-P electrolyte system was constructed, which offered a thermodynamic reason for the direct single-phase HA precipitation observed only in this system, but not in conventional concentrated systems.

  12. Electrochemical deposition mechanism of calcium phosphate coating in dilute Ca-P electrolyte system

    International Nuclear Information System (INIS)

    Hu Ren; Lin Changjian; Shi Haiyan; Wang Hui

    2009-01-01

    In this work, the electrochemical deposition behavior of calcium phosphate coating from an aqueous electrolyte containing very dilute calcium and phosphorus species (Ca-P) was studied. The effects of three process parameters, i.e. temperature, current density and duration, were systematically investigated and the underlying mechanism was thoroughly analyzed. It was observed that the coating is mainly composed of hydroxyapatite (HA) in a wide range of temperature and current densities. The temperature had a significant effect on the deposition velocity. An apparent activation energy of 174.9 kJ mol -1 was subsequently derived, indicating the mass-transfer control mechanism for the coating formation. The current density was identified to be an important parameter for structure controllability. The results of DR-FTIR/Raman spectroscopic studies of the initial deposition phase strongly suggested that the HA coating was instantaneously and directly precipitated on the substrate; neither induction period nor precursor was detected in this dilute Ca-P electrolyte system. Finally, a phase diagram of the Ca-P electrolyte system was constructed, which offered a thermodynamic reason for the direct single-phase HA precipitation observed only in this system, but not in conventional concentrated systems.

  13. Characterization of stem and progenitor cells in the dental pulp of erupted and unerupted murine molars

    Science.gov (United States)

    Balic, Anamaria; Aguila, H. Leonardo; Caimano, Melissa J.; Francone, Victor P.; Mina, Mina

    2010-01-01

    In the past few years there have been significant advances in the identification of putative stem cells also referred to as “mesenchymal stem cells” (MSC) in dental tissues including the dental pulp. It is thought that MSC in dental pulp share certain similarities with MSC isolated from other tissues. However, cells in dental pulp are still poorly characterized. This study focused on the characterization of progenitor and stem cells in dental pulps of erupted and unerupted mice molars. Our study showed that dental pulps from unerupted molars contain a significant number of cells expressing CD90+/CD45-, CD117+/CD45-, Sca-1+/CD45- and little if any CD45+ cells. Our in vitro functional studies showed that dental pulp cells from unerupted molars displayed extensive osteo-dentinogenic potential but were unable to differentiate into chondrocytes and adipocytes. Dental pulp from erupted molars displayed a reduced number of cells, contained higher percentage of CD45+ and lower percentage of cells expressing CD90+/CD45-, CD117+/CD45- as compared to unerupted molars. In vitro functional assays demonstrated the ability of a small fraction of cells to differentiate into odontoblasts, osteoblasts, adipocytes and chondrocytes. There was a significant reduction in the osteo-dentinogenic potential of the pulp cells derived from erupted molars compared to unerupted molars. Furthermore, the adipogenic and chondrogenic differentiation of pulp cells from erupted molars was dependent on a long induction period and infrequent. Based on these findings we propose that the dental pulp of the erupted molars contain a small population of multipotent cells, whereas the dental pulp of the unerupted molars does not contain multipotent cells but is enriched in osteo-dentinogenic progenitors engaged in the formation of coronal and radicular odontoblasts. PMID:20193787

  14. Etude du séchage de la pulpe de safou ; résultats préliminaires

    Directory of Open Access Journals (Sweden)

    Silou, T.

    1991-01-01

    Full Text Available Survey on solar drying of safou pulp. Preliminary results. The consumption of safou, the fruit of Dacryodes edulis is very common in the entire Gulf of Guinea and is subject to a large trade in countries such as Cameroon, Congo, Gabon and Zaire. The fruit is very fragile with a post haverest loss of more than 50 % in the Congo. Solar dring is the only conservation method well managed by the local population but the resulting products have doubious hygenic quality. In order to appreciate the effect of drying on the product quality and in order to improve the traditional processes we have studied both the variation of residual water in the pulp and the acid index and the color of the oil. Drying with a stove (between 70 to 90°C and the solar drying give identical products in spite of some small differences in the dehydration mechanism. The resulting products have satisfying qualities : less than 1 % water content, acide index less than 4, optical density around 0, 3 at 400 nm in 25 g/1 cyclohexane. The product can be directly eaten after rehydration or reused as raw material for the extraction of oil from pulp.

  15. Fatty acids profile of pulp and nuts of Brazilian fruits

    Directory of Open Access Journals (Sweden)

    Paulo Afonso da Costa

    2011-12-01

    Full Text Available Fruits and nuts from the North and Northeast regions of Brazil were collected to determine the fatty acid profile of their oils. The species studied were Brazil (Bertholletia excelsa H.B.K., Mucajá (Couma rigida M., Inajá (Maximiliana maripa D., Jenipapo (Genipa Americana L., and Buriti (Mauritia flexuosa L. nuts. Fatty acid methyl esters were analyzed by gas chromatography with flame ionization detection (GC-FID. Brazil nut major fatty acid was 18:3n-3 (α-linolenic acid, and Buriti nut had approximately 23 times more 18:3n-3 than the pulp. Mucajá nut presented high content of 12:0 (lauric acid and 16:0 (palmitic acid, and Mucajá pulp showed significant levels of 18:2n-6 (linoleic acid. Considering the PUFA (polyunsaturated fatty acid sum values, almost all fruits and nuts analyzed presented very high levels of these compounds. Regarding n-6/n-3 ratio, only Brazil Nut, Buriti Nut, Inajá pulp, and Jenipapo pulp corresponded to the desired profile. These Brazilian fruits and nuts could be of potential interest due to their high nutritive value and lipid content.

  16. Nutritional composition of Annona crassiflora pulp and acceptability of bakery products prepared with its flour

    OpenAIRE

    Villela,Paula; Batista,Ângela Giovana; Dessimoni-Pinto,Nísia Andrade Villela

    2013-01-01

    Annona crassiflora offers an edible fruit native to the Savanna. This study aimed to develop a flour meal from Annona crassiflora pulp; analyze the chemical composition of the fresh pulp and its flour; develop and verify the acceptance of formulations with different concentrations of the flour of Annona crassiflora pulp. Fruit used were selected and processed. The pulp was dried in an oven at 60-65 ºC/48h. We analyzed the chemical composition, and two formulations of breads were prepared with...

  17. Substorms and polar cap convection: the 10 January 2004 interplanetary CME case

    Directory of Open Access Journals (Sweden)

    Y. Andalsvik

    2012-01-01

    Full Text Available The expansion-contraction model of Dungey cell plasma convection has two different convection sources, i.e. reconnections at the magnetopause and in the magnetotail. The spatial-temporal structure of the nightside source is not yet well understood. In this study we shall identify temporal variations in the winter polar cap convection structure during substorm activity under steady interplanetary conditions. Substorm activity (electrojets and particle precipitations is monitored by excellent ground-satellite DMSP F15 conjunctions in the dusk-premidnight sector. We take advantage of the wide latitudinal coverage of the IMAGE chain of ground magnetometers in Svalbard – Scandinavia – Russia for the purpose of monitoring magnetic deflections associated with polar cap convection and substorm electrojets. These are augmented by direct observations of polar cap convection derived from SuperDARN radars and cross-track ion drift observations during traversals of polar cap along the dusk-dawn meridian by spacecraft DMSP F13. The interval we study is characterized by moderate, stable forcing of the magnetosphere-ionosphere system (EKL = 4.0–4.5 mV m−1; cross polar cap potential (CPCP, Φ (Boyle = 115 kV during Earth passage of an interplanetary CME (ICME, choosing an 4-h interval where the magnetic field pointed continuously south-west (Bz By By polarity of the ICME magnetic field, a clear indication of a nightside source.

  18. EFFECTS OF ALKALINE PRE-IMPREGNATION AND PULPING ON MALAYSIA CULTIVATED KENAF (HIBISCUS CANNABINUS

    OpenAIRE

    Lin Suan Ang; Cheu Peng Leh; Chong Chat Lee

    2010-01-01

    This study was carried out to identify an appropriate alkaline pulping condition for Malaysia cultivated kenaf (Hibiscus cannabinus L.). The chemical composition of the kenaf bast and core fibers, and also whole stalk with different growing time were examined prior to pulping attempts. The results of various soda-AQ pulping showed that the degree of carbohydrate degradation and delignification increased with the increase of active alkali and cooking temperature, but decreased with the increas...

  19. Control of the Accumulation of Non-Process Elements in Pulp Mills with Bleach Filtrate Reuse: A Chemical Equilibrium Approach to Predicting the Partitioning of Metals in Pulp Mill and Bleach Plant Streams

    Energy Technology Data Exchange (ETDEWEB)

    Frederick, W.J. Jr.; Rudie, A.W.; Schmidl, G.W.; Sinquefield, S.A.; Rorrer, G.L.; Laver, M.L.; Yantasee, W.; Ming, D.

    2000-08-01

    The overall goal of this project was to develop fundamental, experimentally based methods for predicting the solubility or organic and inorganic matter and their interactions in recycled effluent from kraft pulp mills and bleach plants. This included: characterizing the capacity of wood pulp and dissolved organic matter to bind metal ions, developing a thermodynamic database of properties needed to describe the solubility of inorganic matter in pulp mill streams, incorporation of the database into equilibrium calculation software for predicting the solubility of the metals of interest, and evaluating its capability to predict the distribution of the metals between pulp fibers, inorganic precipitates, and solution.

  20. Discrimination between intact and decayed pulp regions in carious teeth by ADC mapping.

    Science.gov (United States)

    Cankar, Ksenija; Nemeth, Lidija; Bajd, Franci; Vidmar, Jernej; Serša, Igor

    2014-01-01

    The aim of this study was to evaluate an advanced magnetic resonance imaging (MRI) method, apparent diffusion coefficient (ADC) mapping, in the functional assessment of carious teeth. 38 extracted human teeth with scores of 0, 3 and 6 according to International Caries Detection and Assessment System (ICDAS) criteria were screened and subsequently analyzed by MRI at 2.35 T. Histology sectioning of teeth was used for the gold standard by analyzing two extreme cases (intact and severely decayed). ADC maps of the same teeth were calculated from corresponding diffusion-weighted images and used to obtain ADC distributions along dental pulp as functions of the relative pulp length measured from the occlusal pulp side. The measured distributions were analyzed for the best fit by a four-parameter three-segment linear regression model for ADC distribution along the pulp. MRI results were in good agreement with findings in histological sections of identical teeth. The best fit model parameters, relative decayed region depth, relative transition region width and ADC values of intact and decayed pulp tissue, showed statistically significant differences between the ADC values of intact and decayed pulp tissue (1.0 × 10(-9) m(2)/s vs. 0.74-0.89 × 10(-9) m(2)/s) and the relative decayed region depth progressing with ICDAS score (3 vs. 46% with ICDAS 3 vs. ICDAS 6). The results of this feasibility study confirmed relevance of ADC mapping for the discrimination and localization of intact and decayed regions in dental pulps of carious teeth. © 2014 S. Karger AG, Basel.

  1. Interference of apex locator, pulp tester and diathermy on pacemaker function.

    Science.gov (United States)

    Sriman, Narayanan; Prabhakar, V; Bhuvaneswaran, J S; Subha, N

    2015-01-01

    The purpose of this study was to evaluate the effects of three electronic apex locators (EAL), electric pulp tester (EPT) and diathermy on pacemaker function in vitro. Three EALs: Root ZX (J. Morita Co., Tustin, CA, U.S.A.), Propex (Dentsply), Mini Apex locator (SybronEndo, Anaheim, CA, USA), EPT (Parkell pulp vitality tester Farmingdale, NY, USA) and Diathermy (Neomed 250 B) were tested for any interference with one pacemaker (A medtronic kappa KVDD901-serial number: PLE734632S). Directly connecting the pacemaker lead with the EAL/EPT/diathermy operating on a flat bench top, the telemetry wand was held directly over the pacemaker to monitor the pacing pattern for a period of 30 s. Pacemaker activity was continuously recorded on the telemetric programmer and electro gram (EGM) readings examined for pacer inhibition, noise reversion or inappropriate pacemaker pulses. All the three apex locators showed no pacing interference or background noise during its function or at rest. The EGM readings of EPT showed varying levels of background noise in between pacing however, this did not affect the normal pacing pattern and the pacing interval remained constant. EGM readings of diathermy showed an increase in the pacing interval (irregular pacing pattern) followed by complete inhibition of the pacing system. The tested EALs do not interfere with cardiac pacemaker function. The tested EPT showed varying levels of background noise but does not interfere with cardiac pacemaker function. Use of Diathermy interfered with the normal pacing, leading to complete inhibition of the pacing system.

  2. Isolation of Mesenchymal Stem Cells from Human Deciduous Teeth Pulp

    Directory of Open Access Journals (Sweden)

    Aileen I. Tsai

    2017-01-01

    Full Text Available This study aimed to identify predictors of success rate of mesenchymal stem cell (MSC isolation from human deciduous teeth pulp. A total of 161 deciduous teeth were extracted at the dental clinic of Chang Gung Memorial Hospital. The MSCs were isolated from dental pulps using a standard protocol. In total, 128 colonies of MSCs were obtained and the success rate was 79.5%. Compared to teeth not yielding MSCs successfully, those successfully yielding MSCs were found to have less severe dental caries (no/mild-to-moderate/severe: 63.3/24.2/12.5% versus 12.5/42.4/42.4%, P<0.001 and less frequent pulpitis (no/yes: 95.3/4.7% versus 51.5/48.5%, P<0.001. In a multivariate regression model, it was confirmed that the absence of dental caries (OR = 4.741, 95% CI = 1.564–14.371, P=0.006 and pulpitis (OR = 9.111, 95% CI = 2.921–28.420, P<0.001 was significant determinants of the successful procurement of MSCs. MSCs derived from pulps with pulpitis expressed longer colony doubling time than pulps without pulpitis. Furthermore, there were higher expressions of proinflammatory cytokines, interleukin- (IL- 6 and monocyte chemoattractant protein- (MCP- 1, P<0.01, and innate immune response [toll-like receptor 1 (TLR1 and TLR8, P<0.05; TLR2, TLR3, and TLR6, P<0.01] in the inflamed than noninflamed pulps. Therefore, a carious deciduous tooth or tooth with pulpitis was relatively unsuitable for MSC processing and isolation.

  3. Secretoneurin and PE-11 immunoreactivity in the human dental pulp.

    Science.gov (United States)

    Steiner, René; Fischer-Colbrie, Reiner; Bletsa, Athanasia; Laimer, Johannes; Troger, Josef

    2018-02-01

    To explore whether there are differences in the concentration of the secretogranin II-derived peptide secretoneurin and the chromogranin B-derived peptide PE-11 between the healthy and inflamed human dental pulps. Furthermore, colocalization studies with calcitonin gene-related peptide were performed to confirm the sensory origin of the peptidergic nerves in the dental pulp. The concentrations of secretoneurin and PE-11 were determined by highly sensitive radioimmunoassays in extracts of dental pulps, the molecular form of secretoneurin immunoreactivities by RP-HPLC with subsequent radioimmunoassay and colocalization studies with calcitonin gene-related peptide were performed by double immunofluorescence. Only secretoneurin but not PE-11 was detectable by radioimmunoassays whereas nerve fibers could be made visible for both secretoneurin and PE-11. Furthermore, there was a full colocalization of secretoneurin and PE-11 with calcitonin gene-related peptide in immunohistochemical experiments. There were no differences in the concentration of secretoneurin between the healthy and inflamed human dental pulp and moreover, the characterization of the secretoneurin immunoreactivities revealed that only authentic secretoneurin was detected with the secretoneurin antibody. There is unequivocal evidence that secretoneurin and PE-11 are constituents of the sensory innervation of the human dental pulp and although not exclusively but are yet present in unmyelinated C-fibers which transmit predominantly nociceptive impulses. Secretoneurin might be involved in local effector functions as well, particularly in neurogenic inflammation, given that this is the case despite of unaltered levels in inflamed tissue. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Effects of rooting and tree growth of selected woodland species on cap integrity in a mineral capped landfill site.

    Science.gov (United States)

    Hutchings, T R; Moffat, A J; Kemp, R A

    2001-06-01

    The above and below ground growth of three tree species (Alnus glutinosa, Pinus nigra var. maritima and Acer pseudoplatanus) was studied on a containment landfill site at Waterford, Hertfordshire, UK. Tree root architecture was studied using soil inspection pits excavated next to 12 trees of each species and mapped in detail. Tree height was related to soil thickness over the compacted mineral cap. No roots entered the cap where soil thickness was 1.3 m, but a few roots, especially of alder, were observed within it when the soil cover was 1.0 m or less. Micromorphological analysis of undisturbed samples of the mineral cap suggested that roots exploited weaknesses in the cap rather than actively causing penetration into it. Alder roots were more tolerant of anaerobic conditions within the cap than the other species examined. The results confirm that mineral caps should be covered by 1.5 m of soil or soil-forming material if tree establishment is intended over a restored landfill site, unless protected by other parts of a composite capping system.

  5. Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration.

    Science.gov (United States)

    Yasui, T; Mabuchi, Y; Toriumi, H; Ebine, T; Niibe, K; Houlihan, D D; Morikawa, S; Onizawa, K; Kawana, H; Akazawa, C; Suzuki, N; Nakagawa, T; Okano, H; Matsuzaki, Y

    2016-02-01

    Human dental pulp stem/progenitor cells (hDPSCs) are attractive candidates for regenerative therapy because they can be easily expanded to generate colony-forming unit-fibroblasts (CFU-Fs) on plastic and the large cell numbers required for transplantation. However, isolation based on adherence to plastic inevitably changes the surface marker expression and biological properties of the cells. Consequently, little is currently known about the original phenotypes of tissue precursor cells that give rise to plastic-adherent CFU-Fs. To better understand the in vivo functions and translational therapeutic potential of hDPSCs and other stem cells, selective cell markers must be identified in the progenitor cells. Here, we identified a dental pulp tissue-specific cell population based on the expression profiles of 2 cell-surface markers LNGFR (CD271) and THY-1 (CD90). Prospectively isolated, dental pulp-derived LNGFR(Low+)THY-1(High+) cells represent a highly enriched population of clonogenic cells--notably, the isolated cells exhibited long-term proliferation and multilineage differentiation potential in vitro. The cells also expressed known mesenchymal cell markers and promoted new bone formation to heal critical-size calvarial defects in vivo. These findings suggest that LNGFR(Low+)THY-1(High+) dental pulp-derived cells provide an excellent source of material for bone regenerative strategies. © International & American Associations for Dental Research 2015.

  6. Meteorological observations in support of a hill cap cloud experiment

    Energy Technology Data Exchange (ETDEWEB)

    Nielsen, Morten

    1998-06-01

    Humid air flows form a hill cap cloud over the Agana mountain ridge in the north-east of Tenerife. The HILLCLOUD project utilised this cloud formation to investigate the chemical and physical properties of cloud aerosols by land based observations. The project was part of the second Aerosol characterisation Experiment (ACE-2) of the International Global Atmospheric chemistry project (IGAC). The present report describes meteorological observations in support of the hill cap cloud experiment. Time-series of wind speed, wind direction, temperature and humidity were collected at ground-based meteorological stations during a period starting one year in advance of the main campaign. A series of radiosonde detecting the upstream stability and wind profile were launched during the main campaign. (au) 5 tabs., 32 ills., 6 refs.

  7. Application of Alkaline Ionic Liquids in the Pretreatment Process of Eucalyptus Kraft Pulping

    Directory of Open Access Journals (Sweden)

    Yi Hou

    2016-09-01

    Full Text Available In order to explore the potential application of green solvent ionic liquids (ILs in the kraft pulping process, eucalyptus wood was pretreated by [Mmim]DMP before normal pulping. The results showed that materials pretreated shortly by the ionic liquid had a higher yield and viscosity coupled with a lower potassium permanganate value and residual lignin content in the pulp, as a result of the cooking process. It was also inferred that alkaline [Mmim]DMP pretreatment could dissolve lignin effectively from fiber to result in a stronger binding force and more entangled properties. Paper tensile and burst strength were improved by about 40% and 60%, respectively. These results provide a new way for eucalyptus to be utilized in the kraft pulping process.

  8. Analyses of hydraulic performance of velocity caps

    DEFF Research Database (Denmark)

    Christensen, Erik Damgaard; Degn Eskesen, Mark Chr.; Buhrkall, Jeppe

    2014-01-01

    The hydraulic performance of a velocity cap has been investigated. Velocity caps are often used in connection with offshore intakes. CFD (computational fluid dynamics) examined the flow through the cap openings and further down into the intake pipes. This was combined with dimension analyses...

  9. Biomimetic extracellular matrix mediated somatic stem cell differentiation: applications in dental pulp tissue regeneration

    Science.gov (United States)

    Ravindran, Sriram; George, Anne

    2015-01-01

    Dental caries is one of the most widely prevalent infectious diseases in the world. It affects more than half of the world's population. The current treatment for necrotic dental pulp tissue arising from dental caries is root canal therapy. This treatment results in loss of tooth sensitivity and vitality making it prone for secondary infections. Over the past decade, several tissue-engineering approaches have attempted regeneration of the dental pulp tissue. Although several studies have highlighted the potential of dental stem cells, none have transitioned into a clinical setting owing to limited availability of dental stem cells and the need for growth factor delivery systems. Our strategy is to utilize the intact ECM of pulp cells to drive lineage specific differentiation of bone marrow derived mesenchymal stem cells. From a clinical perspective, pulp ECM scaffolds can be generated using cell lines and patient specific somatic stem cells can be used for regeneration. Our published results have shown the feasibility of using pulp ECM scaffolds for odontogenic differentiation of non-dental mesenchymal cells. This focused review discusses the issues surrounding dental pulp tissue regeneration and the potential of our strategy to overcome these issues. PMID:25954205

  10. Steam drying compared to drum drying markedly increases early phase rumen fermentability of sugar beet pulp

    DEFF Research Database (Denmark)

    Nielsen, Mette Olaf; Larsen, Kasper; Jensen, Arne Sloth

    2017-01-01

    Freshly pressed and dried sugar beet pulp was sampled from 2 different factories located within a distance of 30 km and on 4 different dates. One factory was equipped with a steam dryer and the other with a drum dryer. A recognized in vitro technique was used to establish, how the drying process...... affected rumen fermentability of the pulp, since fibrous feeds (such as sugar beet pulp) rely on microbial fermentation in the rumen to be digestible to the cow. Steam dried pulp had a remarkable >60% higher fermentability compared to drum dried pulp during the first 12(-15) hours of fermentation...... (such as pectin) and small particles as low as 6.7-13.3 hours. Future feeding trials are needed to establish exactly how much the feeding value is increased in steam dried sugar beet pulp....

  11. Enzymatic grafting of simple phenols on flax and sisal pulp fibres using laccases.

    Science.gov (United States)

    Aracri, Elisabetta; Fillat, Amanda; Colom, José F; Gutiérrez, Ana; Del Río, José C; Martínez, Angel T; Vidal, Teresa

    2010-11-01

    Flax and sisal pulps were treated with two laccases (from Pycnoporus cinnabarinus, PcL and Trametes villosa, TvL, respectively), in the presence of different phenolic compounds (syringaldehyde, acetosyringone and p-coumaric acid in the case of flax pulp, and coniferaldehyde, sinapaldehyde, ferulic acid and sinapic acid in the case of sisal pulp). In most cases the enzymatic treatments resulted in increased kappa number of pulps suggesting the incorporation of the phenols into fibres. The covalent binding of these compounds to fibres was evidenced by the analysis of the treated pulps, after acetone extraction, by pyrolysis coupled with gas chromatography/mass spectrometry in the absence and/or in the presence of tetramethylammonium hydroxide (TMAH) as methylating agent. The highest extents of phenol incorporation were observed with the p-hydroxycinnamic acids, p-coumaric and ferulic acids. The present work shows for the first time the use of analytical pyrolysis as an effective approach to study fibre functionalization by laccase-induced grafting of phenols. Copyright 2010 Elsevier Ltd. All rights reserved.

  12. Extraction and Characterization of Nanocellulose Structures from Linter Dissolving Pulp Using Ultrafine Grinder.

    Science.gov (United States)

    Ghasemi, Somayeh; Behrooz, Rabi; Ghasemi, Ismail

    2016-06-01

    The objective of this study was to extract cellulose nanofibrils (CNFs) from Linter dissolving pulp through a simple and environmentally friendly physical method of refining pretreatment coupled with ultrafine grinder. The morphology, structure and properties of the Linter pulp and obtained NFCs were investigated using Optical Microscopy (OM), electron microscopy (SEM), Atomic Force Microscopy (AFM), Fourier transformed infrared (FTIR) spectra, X-ray diffraction (XRD) and Thermogravimetric (TG) analysis. The OM results indicate that, the Linter Pulp had length and wide mainly ranged 1.3 mm and 13 μm respectively. Based on AFM images, most of extracted nanocellulose had spherical shape and the average of nanocellulose diameter was varied between 30-70 nm when measured by AFM and SEM. Also the FTIR spectra confirmed that the basic structure of nanocellulose was maintained and no derivative was formed. The X-ray results show that by this method of extraction, the crystallinity index of Nanocellulose isolated (62%) decreased in compared to Linter Pulp (79.5%). Also Linter pulp decomposes at higher temperature (280 degrees C) than isolated nanocellulose (240 degrees C).

  13. Study of pulp chamber morphology of primary mandibular molars using spiral computed tomography.

    Science.gov (United States)

    Selvakumar, Haridoss; Kavitha, Swaminathan; Vijayakumar, Rajendran; Eapen, Thomas; Bharathan, Rajendran

    2014-11-01

    The purpose of this study was to evaluate the pulp chamber morphology in primary mandibular molars using spiral computed tomography (SCT). Sixty extracted primary mandibular molars were scanned using SCT for the (i) distance between the central fissure to furcation, (ii) distance between the central fissure to the floor of the pulp chamber, (iii) height of the pulp chamber (roof-floor), (iv) distance between the floor of the pulp chamber to the furcation. The mean and standard deviation was analyzed using statistical package for the social sciences (SPSS) program. The results of this study show that the average mean distance from the central fissure to the floor of the pulp chamber is 5.07 mm in first mandibular primary molar and 5.54 mm in second mandibular primary molar. It can be concluded that the greatest depth to which a bur can go during access opening without perforating the furcation in first primary mandibular molar is 5.07 mm and 5.54 mm in second primary mandibular molar.

  14. Changes in the composition of the pulp, alpha-amylase activity and ...

    African Journals Online (AJOL)

    The laboratory rotting of Egusi Fruits was completed in 120 hours. At this stage pulp became soft and the seeds were extracted easily with the fingers. The changes in the composition of the pulp, alpha-amylase activity and titratable acidity during the controlled rotting of egusi fruit (Colocynthis citrullus L.) for the harvesting of ...

  15. An environmental systems analysis of the Kraft pulp industry in Thailand

    NARCIS (Netherlands)

    Warit, J.

    2006-01-01

    The pulp industry inThailandis of economic and social importance because of its production value, the revenues from export and the employment in this sector. The eucalyptus-based Kraft pulp industry plays an

  16. 49 CFR 230.41 - Flexible staybolts with caps.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Flexible staybolts with caps. 230.41 Section 230... Appurtenances Staybolts § 230.41 Flexible staybolts with caps. (a) General. Flexible staybolts with caps shall have their caps removed during every 5th annual inspection for the purpose of inspecting the bolts for...

  17. Modeling of thermal and optical effects in dental pulp during the irradiation with neodymium and diode lasers

    International Nuclear Information System (INIS)

    Farhat, Patricia Bahls de Almeida

    2003-01-01

    During the development of applications of high intensity lasers in the enamel and dentine, adverse thermal effects into the entire dental structure, including the pulp, must be verified. The measurement of the temperature in the intact pulp, however, is not a solved problem. For this purpose, models have been used frequently, using extracted teeth, with pulpal cavities filled with materials that simulate only thermal properties of the pulp. Current models, however, do not simulate optical properties of the pulp, not taking the remaining radiation in the pulp chamber into account. The aim of this study was to verify if the remaining radiation from neodymium and diode lasers that reach the pulp chamber, at the models using extracted bovine teeth, can cause local thermal effects. For this purpose, two models were developed, using extracted bovine teeth with their pulp chambers filled with water (simulating pulp thermal characteristics) without (model 1) and with (model 2) an optical absorbent. Models were radiated with 1 W. The obtained results show that, for both lasers, the temperature rise in model 2 pulp chamber is: up to 11 % higher than in the model 1 when the enamel is radiated; and up to 37% higher than in the model 1 when dentine is radiated (1 mm from the pulp), indicating that the level of the remaining radiation is relevant for the construction of models excited by the neodymium and diode lasers. (author)

  18. Anaerobic co-digestion of cyanide containing cassava pulp with pig manure.

    Science.gov (United States)

    Glanpracha, Naraporn; Annachhatre, Ajit P

    2016-08-01

    Anaerobic co-digestion of cyanide-containing cassava pulp with pig manure was evaluated using laboratory scale mesophilic digester. The digester was operated in a semi-continuous mode with the mixed feedstock having C/N ratio of 35:1. Digester startup was accomplished in 60days with loading of 0.5-1kgVS/m(3)d. Subsequently, the loading to digester was increased step-wise from 2 to 9kgVS/m(3)d. Digester performance was stable at loading between 2 and 6kgVS/m(3)d with an average volatile solid removal and methane yield of 82% and 0.38m(3)/kgVSadded, respectively. However, beyond loading of 7kgVS/m(3)d, solubilization of particulate matter did not take place efficiently. Cyanide present in cassava pulp was successfully degraded indicating that anaerobic sludge in the digester was well acclimatized to cyanide. The results show that cassava pulp can be successfully digested anaerobically with pig manure as co-substrate without any inhibitory effect of cyanide present in the cassava pulp. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Effect of irradiation on the dental pulp tissues in streptozotocin-induced diabetic rats

    International Nuclear Information System (INIS)

    Kang, Ho Duk; Hwang, Eui Hwan; Lee, Sang Rae

    2005-01-01

    To observe the histological changes in the pulp tissues of mandibular molars in streptozotocin-induced diabetic rats after irradiation. The male Sprague-Dawley rats weighing approximately 250 gm were divided into four groups : control, diabetes, irradiation, and diabetes-irradiation groups. Diabetes mellitus was induced in the rats by injecting streptozotocin. Rats in control and irradiation groups were injected with citrate buffer only. After 5 days, the head and neck region of the rats in irradiation and diabetes-irradiation groups were irradiated with a single absorbed dose of 10 Gy. All the rats were sacrificed at 3, 7, 14, 21, and 28 days after irradiation. The specimen including the mandibular molars were sectioned and observed using a histopathological method. In the diabetes group, capillary dilatation was observed. However, there was no obvious morphologic alteration of the odontoblasts. In the irradiation group, generalized necrosis of the dental pulp tissues was observed. Vacuolation of the odontoblasts and dilatation of the capillaries were noted in the early experimental phases. In the diabetes-irradiation group, generalized degeneration of the dental pulp tissues was observed. Vacuolation of the dental pulp cells and the odontoblasts was noted in the late experimental phases. This experiment suggest that dilatation of the capillaries in the dental pulp tissue is induced by diabetic state, and generalized degeneration of the dental pulp tissues is induced by irradiation of the diabetic group.

  20. An in vitro evaluation of cytotoxicity of curcumin against human dental pulp fibroblasts

    Directory of Open Access Journals (Sweden)

    Praveenkumar S Mandrol

    2016-01-01

    Full Text Available Objective: The objective of this study was to evaluate the cytotoxicity of curcumin to primary dental pulp fibroblasts in vitro. Materials and Methods: Dental pulp fibroblasts from primary maxillary central incisors were cultured and used for cytotoxicity tests after the fourth passage. Ninety-five percent curcumin was diluted with dimethylsulfoxide to prepare 100%, 50%, and 25% concentrations. Each concentration of curcumin was added in triplicate into 96-well microtiter plate containing the fibroblast culture at 104/well. Cells without treatment served as a control group. The number of viable cells after 48 hrs incubation at 37°C in a humidified atmosphere of 5 % CO2 and 95 % air was determined by the 3-(4, 5-dimethyl-thiazol-2-yl-2, 5-diphenyl-tetrazolium bromide (MTT assay. The relative viability of pulp cells was expressed as color intensity of the number in the experimental wells relative to that of the control group. Absorbances were read at 492 nm on a microplate reader with a background subtraction at 620 nm. Results: Cell viability of primary dental pulp fibroblasts to 25%, 50%, and 100% curcumin concentration was 174%, 310%, and 317%, respectively. Conclusions: Curcumin promotes cell viability and induces proliferation of primary dental pulp fibroblasts and has the potential to be developed into an economical and reliable medicament for vital pulp therapy.