Travelers' Health: Diphtheria

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... membrane tissue. Diphtheria is a nationally notifiable disease. TREATMENT Patients with respiratory diphtheria require hospitalization to monitor ... Jan 20;81(3):24–32. Chapter 3 - Dengue Chapter 3 - Ebola Virus Disease & Marburg Virus Disease ...

Epidemiology of diphtheria in South Dakota.

Science.gov (United States)

Golaz, A; Lance-Parker, S; Welty, T; Schaefer, L; Volmer, L; LaFromboise, C; Dixon, J; Haase, T; Kim, C; Popovic, T; Bisgard, K; Strebel, P; Wharton, M

2000-07-01

Respiratory diphtheria was one of the most common causes of death among children in the pre-vaccine era. Since the introduction of diphtheria toxoid vaccine in 1920s, and its widespread use by the late 1940s, diphtheria became increasingly rare in the United States. However, through the 1970s diphtheria remained endemic in some states, with reported incidence rates > 1.0 per million population in six states (Alaska, Arizona, Montana, New Mexico, South Dakota, and Washington). Starting in 1980, less than five cases have been reported each year in the United States. The majority of culture-confirmed cases have been associated with importation from other countries. Toxigenic Corynebacterium diphtheriae, the organism causing diphtheria, was thought to have become rare or even have disappeared from previously endemic areas such as South Dakota. However, during four months in 1996, 11 persons (one index case, six patients and four household contacts) in an American Indian community in South Dakota were found to be infected by C. diphtheriae; six of these isolates were toxigenic. The findings in this report indicate that despite 20 years without reported respiratory diphtheria cases, toxigenic C. diphtheriae is still present in South Dakota. The continuous circulation of toxigenic strains of C. diphtheriae emphasizes the need for health care providers throughout South Dakota to promote timely vaccination against diphtheria among persons of all ages and ethnic groups, to be aware of the clinical signs and symptoms of diphtheria so that cases can be promptly diagnosed and treated, and further public health measures can be taken to contain this serious disease.

Determinants of Clinical Diphtheria After Sub National Diphtheria Immunization Days In 2012 At Bangkalan

Directory of Open Access Journals (Sweden)

Feranita Utama

2014-01-01

Full Text Available ABSTRACT The government had held sub-national diphtheria immunization days in 2012 to combat an outbreak of diphtheria in East Java province. However, there werestill regencies or cities which had high incidence and Bangkalanregencywas the highest. The research aimed to analyze the determinants of diphtheria in Bangkalan after SNIDs. This research was case control study with 31 subjects as case and 124 as control spread in 25 case villages and 25 control villages. Bivariate analysis used chi-square and simple logistic regression. Multivariate analysis used logistic regression. Bivariate analysisresult showed that individual and household variables that had associate with incidence of diphtheria in the Bangkalan after SNIDs of diphtheria vaccine in 2012 were DPT immunization status, the status of SNIDs, age, and mother's education level. In this study, there were not associate between village variables with diphtheria in the Bangkalan after SNIDs. Multivariate analysis result showed that had associate with incidence of diphtheria in Bangkalan after SNIDs were no DPT immunization (p=0,012; OR=4,765, incomplete DPT immunization (p=0,001; OR=6,276, 3-7 years of age (p=0,014; OR=15,137, 7-15 years of age (p=0,001;OR=41,984, and no immunization in SNIDs (p=0,020; OR=3,553. Conclusion, the dominantfactors that affected diphtheriainBangkalanwere status of DPTimmunization, ageand the SNIDs of diphtheria. Recommendation, DPTimmunizationshould becomplete, boosterimmunization should be needed, monitoring theimplementation ofsubPIN should be strict, especially inschool, and thecoverage ofDPT3andsubPIN should be increased and its report should be improved. Keywords: diphtheria, sub-nationaldiphtheriaimmunization days, DPT                              immunization

Corynebacterium diphtheriae in a free-roaming red fox: case report and historical review on diphtheria in animals.

Science.gov (United States)

Sing, Andreas; Konrad, Regina; Meinel, Dominik M; Mauder, Norman; Schwabe, Ingo; Sting, Reinhard

2016-08-01

Corynebacterium diphtheriae, the classical causative agent of diphtheria, is considered to be nearly restricted to humans. Here we report the first finding of a non-toxigenic C. diphtheriae biovar belfanti strain in a free-roaming wild animal. The strain obtained from the subcutis and mammary gland of a dead red fox (Vulpes vulpes) was characterized by biochemical and molecular methods including MALDI-TOF and Multi Locus Sequence Typing. Since C. diphtheriae infections of animals, usually with close contact to humans, are reported only very rarely, an intense review comprising also scientific literature from the beginning of the 20th century was performed. Besides the present case, only 11 previously reported C. diphtheriae animal infections could be verified using current scientific criteria. Our report is the first on the isolation of C. diphtheriae from a wildlife animal without any previous human contact. In contrast, the very few unambiguous publications on C. diphtheriae in animals referred to livestock or pet animals with close human contact. C. diphtheriae carriage in animals has to be considered as an exceptionally rare event.

Diphtheria in Mayotte, 2007-2015.

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Belchior, Emmanuel; Henry, Sabine; Badell, Edgar; Collet, Louis; Benoit-Cattin, Thierry; de Montera, Anne-Marie; Guiso, Nicole; Patey, Olivier; Levy-Bruhl, Daniel; Filleul, Laurent; Chieze, Francois; Olivier, Sophie

2017-07-01

Epidemiology of diphtheria in the southwestern Indian Ocean is poorly documented. We analyzed 14 cases of infection with toxigenic Corynebacterium diphtheriae reported during 2007-2015 in Mayotte, a French department located in this region. Local control of diphtheria is needed to minimize the risk for importation of the bacterium into disease-free areas.

Structural conservation, variability, and immunogenicity of the T6 backbone pilin of serotype M6 Streptococcus pyogenes.

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Young, Paul G; Moreland, Nicole J; Loh, Jacelyn M; Bell, Anita; Atatoa Carr, Polly; Proft, Thomas; Baker, Edward N

2014-07-01

Group A streptococcus (GAS; Streptococcus pyogenes) is a Gram-positive human pathogen that causes a broad range of diseases ranging from acute pharyngitis to the poststreptococcal sequelae of acute rheumatic fever. GAS pili are highly diverse, long protein polymers that extend from the cell surface. They have multiple roles in infection and are promising candidates for vaccine development. This study describes the structure of the T6 backbone pilin (BP; Lancefield T-antigen) from the important M6 serotype. The structure reveals a modular arrangement of three tandem immunoglobulin-like domains, two with internal isopeptide bonds. The T6 pilin lysine, essential for polymerization, is located in a novel VAKS motif that is structurally homologous to the canonical YPKN pilin lysine in other three- and four-domain Gram-positive pilins. The T6 structure also highlights a conserved pilin core whose surface is decorated with highly variable loops and extensions. Comparison to other Gram-positive BPs shows that many of the largest variable extensions are found in conserved locations. Studies with sera from patients diagnosed with GAS-associated acute rheumatic fever showed that each of the three T6 domains, and the largest of the variable extensions (V8), are targeted by IgG during infection in vivo. Although the GAS BP show large variations in size and sequence, the modular nature of the pilus proteins revealed by the T6 structure may aid the future design of a pilus-based vaccine. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Tetanus, Diphtheria, Pertussis (Tdap) Vaccine

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Adacel® (as a combination product containing Diphtheria, Tetanus Toxoids, Acellular Pertussis Vaccine) ... Boostrix® (as a combination product containing Diphtheria, Tetanus Toxoids, Acellular Pertussis Vaccine)

Radioimmunodiffusion technique for determining diphtheria antitoxin

International Nuclear Information System (INIS)

Maniar, A.C.

1977-01-01

A radioimmunodiffusion technique for detecting low levels of diphtheria antitoxin was developed. Diphtheria toxoid was labelled with 125 I to facilitate detection of lines of precipitation by the use of X-ray film, the lower limit of detection being 0.001 unit per millilitre of diphtheria antitoxin. (author)

Identification of a human monoclonal antibody to replace equine diphtheria antitoxin for treatment of diphtheria intoxication.

Science.gov (United States)

Sevigny, Leila M; Booth, Brian J; Rowley, Kirk J; Leav, Brett A; Cheslock, Peter S; Garrity, Kerry A; Sloan, Susan E; Thomas, William; Babcock, Gregory J; Wang, Yang

2013-11-01

Diphtheria antitoxin (DAT) has been the cornerstone of the treatment of Corynebacterium diphtheriae infection for more than 100 years. Although the global incidence of diphtheria has declined steadily over the last quarter of the 20th century, the disease remains endemic in many parts of the world, and significant outbreaks still occur. DAT is an equine polyclonal antibody that is not commercially available in the United States and is in short supply globally. A safer, more readily available alternative to DAT would be desirable. In the current study, we obtained human monoclonal antibodies (hMAbs) directly from antibody-secreting cells in the circulation of immunized human volunteers. We isolated a panel of diverse hMAbs that recognized diphtheria toxoid, as well as a variety of recombinant protein fragments of diphtheria toxin. Forty-five unique hMAbs were tested for neutralization of diphtheria toxin in in vitro cytotoxicity assays with a 50% effective concentration of 0.65 ng/ml for the lead candidate hMAb, 315C4. In addition, 25 μg of 315C4 completely protected guinea pigs from intoxication in an in vivo lethality model, yielding an estimated relative potency of 64 IU/mg. In comparison, 1.6 IU of DAT was necessary for full protection from morbidity and mortality in this model. We further established that our lead candidate hMAb binds to the receptor-binding domain of diphtheria toxin and physically blocks the toxin from binding to the putative receptor, heparin-binding epidermal growth factor-like growth factor. The discovery of a specific and potent human neutralizing antibody against diphtheria toxin holds promise as a potential therapeutic.

Diphtheria outbreak in Thailand, 2012; seroprevalence of diphtheria antibodies among Thai adults and its implications for immunization programs.

Science.gov (United States)

Wanlapakorn, Nasamon; Yoocharoen, Pornsak; Tharmaphornpilas, Piyanit; Theamboonlers, Apiradee; Poovorawan, Yong

2014-09-01

An age distribution shift in diphtheria cases during a 2012 outbreak in northeastern of Thailand suggests adults are increasingly at risk for infection in Thailand. Data regarding immunity against diphtheria among the adult Thai population is limited. We review a 2012 diphtheria outbreak in Thailand and conducted a nationwide seroepidemiological survey to determine the prevalence of diphtheria antibodies among Thai adults in order to inform immunization programs. A total of 41 confirmed cases, 6 probable cases and 101 carriers of diphtheria were reported from northeastern and upper southern Thailand. The diphtheria outbreak in northeastern Thailand occurred among adults aged > or =15 years; sporadic cases occurred among children from upper southern Thailand. We conducted a seroepidemiological survey of 890 Thai adults from 4 age groups (20-29, 30-39, 40-49 and 50-59 years) in 7 different geographical areas of Thailand (Chiang Mai, Ratchaburi, Chon Buri, Nakhon Si Thammarat, Phitsanulok, Khon Kaen and Songkhla). Diptheria toxin antibody levels were measured with a commercially available ELISA test. The seroprotection rate ranged from 83% to 99%, with the highest in eastern Thailand (Chon Buri, 99%) and the lowest in northern Thailand (Chiang Mai, 83%). Diphtheria antibodies declined with increasing age. We recommend one doseof diphtheria-tetanus toxoid (dT) vaccine once after 20 years of age in order to boost the antibody and revaccinations every 10 years to prevent future outbreaks.

Corynebacterium ulcerans cutaneous diphtheria.

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Moore, Luke S P; Leslie, Asuka; Meltzer, Margie; Sandison, Ann; Efstratiou, Androulla; Sriskandan, Shiranee

2015-09-01

We describe the case of a patient with cutaneous diphtheria caused by toxigenic Corynebacterium ulcerans who developed a right hand flexor sheath infection and symptoms of sepsis such as fever, tachycardia, and elevated C-reactive protein, after contact with domestic cats and dogs, and a fox. We summarise the epidemiology, clinical presentation, microbiology, diagnosis, therapy, and public health aspects of this disease, with emphasis on improving recognition. In many European countries, C ulcerans has become the organism commonly associated with cutaneous diphtheria, usually seen as an imported tropical disease or resulting from contact with domestic and agricultural animals. Diagnosis relies on bacterial culture and confirmation of toxin production, with management requiring appropriate antimicrobial therapy and prompt administration of antitoxin, if necessary. Early diagnosis is essential for implementation of control measures and clear guidelines are needed to assist clinicians in managing clinical diphtheria. This case was a catalyst to the redrafting of the 2014 national UK interim guidelines for the public health management of diphtheria, released as final guidelines in March, 2015. Copyright © 2015 Elsevier Ltd. All rights reserved.

Diphtheria: It is still prevalent!!!

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Jain, Avani; Samdani, Sunil; Meena, Vinod; Sharma, Man Prakash

2016-07-01

Diphtheria is a respiratory infectious disease of childhood. It is a fatal disease and may cause complications if not recognized early and treated properly. Despite availability of effective vaccination it continues to be reported from many parts of the world particularly developing countries. To assess the demographic and clinical characteristics of diphtheria patients, and the predictors of outcomes of respiratory diphtheria. A prospective analysis of 180 patients with a clinical diagnosis of respiratory diphtheria admitted from 2011 to 2014 at a tertiary referral hospital. They were evaluated with respect to demographic details, immunization status, clinical features, complications and outcomes. Most common age group affected was children less than 5 years of age (87 cases, 48.33%). The peak incidence of diphtheria was seen in the months of September and October (111 cases, 62%). Majority of the patients were unimmunized (54%), followed by partially immunized (21%). The most common complication was respiratory in 80 cases (44%), followed by cardiac complications in 54 cases (30%), and renal (16%) and neurological complications (10%). Cardiac complications were associated with the highest mortality rate (63%). The presence of bull neck and pseudomembrane score >2 was associated with a high mortality. Diphtheria is still a preventable public health problem in many developing countries. Improved vaccination coverage, including booster dosage, coupled with early detection and effective treatment, may all reduce incidence and mortality. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Diphtheria in the Republic of Georgia: Use of Molecular Typing Techniques for Characterization of Corynebacterium diphtheriae Strains

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Sulakvelidze, Alexander; Kekelidze, Merab; Gomelauri, Tsaro; Deng, Yingkang; Khetsuriani, Nino; Kobaidze, Ketino; De Zoysa, Aruni; Efstratiou, Androulla; Morris, J. Glenn; Imnadze, Paata

1999-01-01

Sixty-six Corynebacterium diphtheriae strains (62 of the gravis biotype and 4 of the mitis biotype) isolated during the Georgian diphtheria epidemic of 1993 to 1998 and 13 non-Georgian C. diphtheriae strains (10 Russian and 3 reference isolates) were characterized by (i) biotyping, (ii) toxigenicity testing with the Elek assay and PCR, (iii) the randomly amplified polymorphic DNA (RAPD) technique, and (iv) pulsed-field gel electrophoresis (PFGE). Fifteen selected strains were ribotyped. Six RAPD types and 15 PFGE patterns were identified among all strains examined, and 12 ribotypes were found among the 15 strains that were ribotyped. The Georgian epidemic apparently was caused by one major clonal group of C. diphtheriae (PFGE type A, ribotype R1), which was identical to the predominant epidemic strain(s) isolated during the concurrent diphtheria epidemic in Russia. A dendrogram based on the PFGE patterns revealed profound differences between the minor (nonpredominant) epidemic strains found in Georgia and Russia. The methodologies for RAPD typing, ribotyping, and PFGE typing of C. diphtheriae strains were improved to enable rapid and convenient molecular typing of the strains. The RAPD technique was adequate for biotype differentiation; however, PFGE and ribotyping were better (and equal to each other) at discriminating between epidemiologically related and unrelated isolates. PMID:10488190

Neurologic manifestations of diphtheria and pertussis.

Science.gov (United States)

Sanghi, Viraj

2014-01-01

Historically, diphtheria was a major cause of morbidity and mortality in the prevaccine era. However, in recent times there has been a resurgence of diphtheria, especially in the newly independent states of the former USSR. Diphtheritic polyneuropathy can be a serious complication in patients who have a severe infection. In patients with pertussis, seizures and encephalopathy can occur as a complication of asphyxia. Vaccination against diphtheria and pertussis in children and booster vaccination in adults is recommended. DTP (diphtheria, tetanus, pertussis) vaccination has been shown to increase the risk of febrile seizures in children. Currently, it appears that the risk of vaccine-induced encephalopathy and/or epilepsy following DTP vaccination, if any, is extremely low. © 2014 Elsevier B.V. All rights reserved.

STUDY OF CARDIAC INVOLVEMENT IN DIPHTHERIA

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Bala Raju Gundam

2016-07-01

Full Text Available BACKGROUND Diphtheria still endemic in many developing countries and is responsible for high case fatality. Myocardial involvement is a serious complication of diphtheria and diphtheria myocarditis frequently complicated by arrhythmias causes sudden death if not managed properly. So, early and adequate administration of Anti-Diphtheritic Serum (ADS and frequent monitoring particularly 24 hrs. ECG monitoring is helpful to detect any serious cardiac arrhythmias. METHODS A prospective study on patients who were culture positive diagnosed on the basis of WHO definition were treated and analysed in respect to homographic details, immunisation strategy, and clinical features with particular attention to myocarditis. RESULTS In the study, common age group affected by diphtheria was 5 to 10 years. Incidence of myocarditis more in same age group. Out of 64 patients with myocarditis, 23 patients died. CONCLUSION Incidence of diphtheria has increased in the recent years. Commonly effected age group is 5 to 10 years and most of them are presented with asymptomatic myocarditis. Symptomatic diphtheritic myocarditis has high mortality rate even with aggressive management. Patient who presented with either LBBB alone are with combination of other conduction of abnormalities has high mortality rate.

Diphtheria in the postepidemic period, Europe, 2000-2009.

Science.gov (United States)

Wagner, Karen S; White, Joanne M; Lucenko, Irina; Mercer, David; Crowcroft, Natasha S; Neal, Shona; Efstratiou, Androulla

2012-02-01

Diphtheria incidence has decreased in Europe since its resurgence in the 1990s, but circulation continues in some countries in eastern Europe, and sporadic cases have been reported elsewhere. Surveillance data from Diphtheria Surveillance Network countries and the World Health Organization European Region for 2000-2009 were analyzed. Latvia reported the highest annual incidence in Europe each year, but the Russian Federation and Ukraine accounted for 83% of all cases. Over the past 10 years, diphtheria incidence has decreased by >95% across the region. Although most deaths occurred in disease-endemic countries, case-fatality rates were highest in countries to which diphtheria is not endemic, where unfamiliarity can lead to delays in diagnosis and treatment. In western Europe, toxigenic Corynebacterium ulcerans has increasingly been identified as the etiologic agent. Reduction in diphtheria incidence over the past 10 years is encouraging, but maintaining high vaccination coverage is essential to prevent indigenous C. ulcerans and reemergence of C. diphtheriae.

Mathematical modeling of diphtheria transmission in Thailand.

Science.gov (United States)

Sornbundit, Kan; Triampo, Wannapong; Modchang, Charin

2017-08-01

In this work, a mathematical model for describing diphtheria transmission in Thailand is proposed. Based on the course of diphtheria infection, the population is divided into 8 epidemiological classes, namely, susceptible, symptomatic infectious, asymptomatic infectious, carrier with full natural-acquired immunity, carrier with partial natural-acquired immunity, individual with full vaccine-induced immunity, and individual with partial vaccine-induced immunity. Parameter values in the model were either directly obtained from the literature, estimated from available data, or estimated by means of sensitivity analysis. Numerical solutions show that our model can correctly describe the decreasing trend of diphtheria cases in Thailand during the years 1977-2014. Furthermore, despite Thailand having high DTP vaccine coverage, our model predicts that there will be diphtheria outbreaks after the year 2014 due to waning immunity. Our model also suggests that providing booster doses to some susceptible individuals and those with partial immunity every 10 years is a potential way to inhibit future diphtheria outbreaks. Copyright © 2017 Elsevier Ltd. All rights reserved.

Diphtheria outbreak with high mortality in northeastern Nigeria.

Science.gov (United States)

Besa, N C; Coldiron, M E; Bakri, A; Raji, A; Nsuami, M J; Rousseau, C; Hurtado, N; Porten, K

2014-04-01

SUMMARY A diphtheria outbreak occurred from February to November 2011 in the village of Kimba and its surrounding settlements, in Borno State, northeastern Nigeria. We conducted a retrospective outbreak investigation in Kimba village and the surrounding settlements to better describe the extent and clinical characteristics of this outbreak. Ninety-eight cases met the criteria of the case definition of diphtheria, 63 (64.3%) of whom were children aged diphtheria. None of the 98 cases received diphtheria antitoxin, penicillin, or erythromycin during their illness. The overall case-fatality ratio was 21.4%, and was highest in children aged 0-4 years (42.9%). Low rates of immunization, delayed clinical recognition of diphtheria and absence of treatment with antitoxin and appropriate antibiotics contributed to this epidemic and its severity.

Diphtheria, Tetanus, and Pertussis (DTaP) Vaccine

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Certiva® (as a combination product containing Diphtheria, Tetanus Toxoids, Acellular Pertussis Vaccine) ... Daptacel® (as a combination product containing Diphtheria, Tetanus Toxoids, Acellular Pertussis Vaccine)

Features of Diphtheria in Children

Directory of Open Access Journals (Sweden)

O.K. Koloskova

2014-09-01

Full Text Available The lecture describes the etiology, epidemiology, classification and clinical features of diphtheria in children, taking into account various sites, covers the issues of diagnosis. Treatment for diphtheria in children is carried as per the order of Ministry of Healthcare of Ukraine № 354 dated July 9, 2004 in accordance with the form and severity of the disease.

Epidemiology of Diphtheria in India, 1996-2016: Implications for Prevention and Control.

Science.gov (United States)

Murhekar, Manoj

2017-08-01

Diphtheria is an acute disease caused by exotoxin-producing Corynebacterium diphtheriae . Globally, diphtheria has been showing a declining trend due to effective childhood vaccination programs. A substantial proportion of global burden of diphtheria is contributed by India. Hospital-based surveillance studies as well as diphtheria outbreaks published in last 20 years (1996-2016) indicate that diphtheria cases are frequent among school-going children and adolescents. In some Indian states, Muslim children are affected more. As per the national level health surveys, coverage of three doses of diphtheria vaccine was 80% during 2015-2016. Information about coverage of diphtheria boosters is not routinely collected through these surveys, but is expected to be low. Few studies also indicate low diphtheria immunity among school-going children and adults. The strategies for prevention of diphtheria need to focus on improving coverage of primary and booster doses of diphtheria vaccines administered as a part of Universal Immunization Program as well as introducing diphtheria vaccine for school-going children.

Diphtheria - 'The strangling angel' of children.

Science.gov (United States)

Byard, Roger W

2013-02-01

Diphtheria, an acute infectious condition caused by Corynebacterium diphtheriae, was once a major killer of children. Although the mortality rates dropped dramatically in the mid-twentieth century, due to a combination of improved standards of living and immunization programs, outbreaks are still occurring. Two children, aged four and five years respectively, are reported to demonstrate characteristic features of lethal cases. Death in case 1 was due to an extensive upper airway pseudomembrane causing acute respiratory failure. The diagnosis of diphtheria was only made at postmortem. Death in case 2 was due to acute cardiac failure with heart block complicating diphtheria. Other mechanisms in fatal cases involve disseminated intravascular coagulation, renal and endocrine failure. Declining levels of immunity among adults has resulted in a change in the epidemiological pattern of the disease with an older age of victims in recent outbreaks. As a result of population shifts and failure to immunize children it is likely that forensic pathologists may see more cases of diphtheria in the future. Due to the rarity of cases in Western communities and atypical presentations, the diagnosis may only be established at autopsy. Copyright © 2012 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

An isolated outbreak of diphtheria in South Africa, 2015.

Science.gov (United States)

Mahomed, S; Archary, M; Mutevedzi, P; Mahabeer, Y; Govender, P; Ntshoe, G; Kuhn, W; Thomas, J; Olowolagba, A; Blumberg, L; McCarthy, K; Mlisana, K; DU Plessis, M; VON Gottberg, A; Moodley, P

2017-07-01

An outbreak of respiratory diphtheria occurred in two health districts in the province of KwaZulu-Natal in South Africa in 2015. A multidisciplinary outbreak response team was involved in the investigation and management of the outbreak. Fifteen cases of diphtheria were identified, with ages ranging from 4 to 41 years. Of the 12 cases that were under the age of 18 years, 9 (75%) were not fully immunized for diphtheria. The case fatality was 27%. Ninety-three household contacts, 981 school or work contacts and 595 healthcare worker contacts were identified and given prophylaxis against Corynebacterium diphtheriae infection. A targeted vaccination campaign for children aged 6-15 years was carried out at schools in the two districts. The outbreak highlighted the need to improve diphtheria vaccination coverage in the province and to investigate the feasibility of offering diphtheria vaccines to healthcare workers.

The changing epidemiology of diphtheria in Jordan*

OpenAIRE

Khuri-Bulos, N.; Hamzah, Y.; Sammerrai, S. M.; Shehabi, A.; Hamed, R.; Arnaout, M. A.; Turk, J.; Qubain, H.

1988-01-01

Outbreaks of diphtheria used to occur regularly in Jordan, the last such outbreak being in 1977-78. Since that time, a massive immunization programme targeted at pre-school-age children has been markedly successful. Hence, when an outbreak of diphtheria occurred in 1982-83, it was unexpected. Of the 35 patients who were treated at the Jordan University Hospital, two died and the remaining 33 recovered uneventfully. Contrary to our findings in previous diphtheria epidemics in Jordan, this outb...

[First confirmed case of laryngeal diphtheria in Djibouti].

Science.gov (United States)

Koeck, J L; Merle, C; Bimet, F; Kiredjian, M; Goullin, B; Teyssou, R

2000-01-01

The first bacteriologically confirmed case of laryngeal diphtheria in Djibouti was reported in 1998. It involved a three-year-old native-born infant who had been vaccinated during the first year of life with three doses of a combined vaccine against diphtheria, tetanus, poliomyelitis, and pertussis. A rapid clinical improvement was observed under erythromycin treatment. Other cases of laryngeal diphtheria have been observed. It is important to reverse decreasing vaccinal coverage in Djibouti and to warn incoming travelers of the need to be adequate immunized against diphtheria. Enhanced epidemiologic surveillance of this disease is also needed.

Evaluation of diphtheria convalescent patients to serve as donors for the production of anti-diphtheria immunoglobulin preparations

NARCIS (Netherlands)

Bissumbhar, B.; Rakhmanova, A.G.; Berbers, G.; Iakolev, A.; Nosikova, E.; Melnick, O.; Ovtcharenko, E.; Rümke, H. C.; Ruitenberg, E.J.

2004-01-01

Aims: The study was conducted to evaluate the possibility of selecting convalescent diphtheria patients to serve in emergency situations as donors for the production of anti-diphtheria immunoglobulin. To select suitable donors, the criterion of an antitoxin titer ≥3.0 IU/ml was used. In addition,

The changing epidemiology of diphtheria in Jordan*

Science.gov (United States)

Khuri-Bulos, N.; Hamzah, Y.; Sammerrai, S. M.; Shehabi, A.; Hamed, R.; Arnaout, M. A.; Turk, J.; Qubain, H.

1988-01-01

Outbreaks of diphtheria used to occur regularly in Jordan, the last such outbreak being in 1977-78. Since that time, a massive immunization programme targeted at pre-school-age children has been markedly successful. Hence, when an outbreak of diphtheria occurred in 1982-83, it was unexpected. Of the 35 patients who were treated at the Jordan University Hospital, two died and the remaining 33 recovered uneventfully. Contrary to our findings in previous diphtheria epidemics in Jordan, this outbreak largely involved adolescents and young adults. PMID:3260143

Postbooster Antibodies from Humans as Source of Diphtheria Antitoxin.

Science.gov (United States)

Bermejo-Martin, Jesús F; Avila-Alonso, Ana; González-Rivera, Milagros; Tamayo, Eduardo; Eiros, Jose María; Almansa, Raquel

2016-07-01

Diphtheria antitoxin for therapeutic use is in limited supply. A potential source might be affinity-purified antibodies originally derived from plasma of adults who received a booster dose of a vaccine containing diphtheria toxoid. These antibodies might be useful for treating even severe cases of diphtheria.

Diphtheria in the Postepidemic Period, Europe, 2000–2009

Science.gov (United States)

White, Joanne M.; Lucenko, Irina; Mercer, David; Crowcroft, Natasha S.; Neal, Shona; Efstratiou, Androulla

2012-01-01

Diphtheria incidence has decreased in Europe since its resurgence in the 1990s, but circulation continues in some countries in eastern Europe, and sporadic cases have been reported elsewhere. Surveillance data from Diphtheria Surveillance Network countries and the World Health Organization European Region for 2000–2009 were analyzed. Latvia reported the highest annual incidence in Europe each year, but the Russian Federation and Ukraine accounted for 83% of all cases. Over the past 10 years, diphtheria incidence has decreased by >95% across the region. Although most deaths occurred in disease-endemic countries, case-fatality rates were highest in countries to which diphtheria is not endemic, where unfamiliarity can lead to delays in diagnosis and treatment. In western Europe, toxigenic Corynebacterium ulcerans has increasingly been identified as the etiologic agent. Reduction in diphtheria incidence over the past 10 years is encouraging, but maintaining high vaccination coverage is essential to prevent indigenous C. ulcerans and reemergence of C. diphtheriae infections. PMID:22304732

Cardiac complications in diphtheria and predictors of outcomes.

Science.gov (United States)

Samdani, Sunil; Jain, Avani; Meena, Vinod; Meena, C B

2018-01-01

To study the cardiac complications in diphtheria patients and to study the predictors of outcomes. Single centre prospective analysis of cardiac complications in diphtheria patients. In this study, there were 60 patients diagnosed with diphtheria with ECG changes. The ECG changes seen were sinus tachycardia (68.3%), T wave inversion (20%), ST segment depression (13.3%), right bundle branch block (5%), multiple atrial ectopics (3.3%). The case fatality rate in our study was 25% (15 patients). High CPK-MB, myoglobulin and cardiac troponin levels were associated with cardiac mortality. In our study, cardiac troponin T had the highest sensitivity (80%) and CK-MB had the highest specificity (95.56%). Cardiac involvement is a common complication of infection with C. diphtheria and is associated with high mortality. As diphtheria can be prevented by adequate vaccination, efforts should be maximized for high vaccine coverage with booster doses. Copyright © 2017. Published by Elsevier B.V.

Sudden death of a child due to respiratory diphtheria.

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Swain, Rajanikanta; Behera, Chittaranjan; Arava, Sudheer Kumar; Kundu, Naveen

2016-06-01

A four-year-old girl presented to the emergency department with respiratory distress. Death occurred despite attempted resuscitation. The illness was not clinically diagnosed. Her father revealed that she had a fever and sore throat for the last four days and was not immunised for diphtheria. Characteristic gross and microscopic pathology of respiratory diphtheria and microbiological findings were observed. The cause of death was acute respiratory failure consequent upon upper airway obstruction from diphtheria. Forensic pathologists should remember that the diphtheria cases can cause sudden death especially in developing countries. © The Author(s) 2016.

Quantitative estimation of diphtheria and tetanus toxoids. 4. Toxoids as international reference materials defining Lf-units for diphtheria and tetanus toxoids.

Science.gov (United States)

Lyng, J

1990-01-01

The Lf-unit, which is used in the control of diphtheria and tetanus toxoid production and in some countries also to follow immunization of horses for production of antitoxins, has hitherto been defined by means of antitoxin preparations. A diphtheria toxoid and a tetanus toxoid preparation, both freeze-dried, were examined in an international collaborative study for their suitability to serve as reference reagents in the flocculation tests and for defining the Lf-units. It was shown that flocculation tests using the reference toxoids are very reproducible and reliable and the WHO Expert Committee on Biological Standardization established: the toxoid called DIFT as the International Reference Reagent of Diphtheria Toxoid for Flocculation Test with a defined content of 900 Lf-units of diphtheria toxoid per ampoule; and the toxoid called TEFT as the International Reference Reagent of Tetanus Toxoid for Flocculation Test with a defined content of 1000 Lf-units of diphtheria toxoid per ampoule.

Diphtheria in Andhra Pradesh-a clinical-epidemiological study.

Science.gov (United States)

M, Meera; M, Rajarao

2014-02-01

Clinical diphtheria is on the increase worldwide, mainly affecting developing countries. We sought to understand its presentation among patients at Sir Ronald Ross Institute of Tropical and Communicable Diseases in Hyderabad, Andhra Pradesh, India. Diphtheria patients presented with fever, pharyngitis, and a patch in the throat. Data collected for each patient included age, clinical presentation, morbidity, mortality, bacteria isolated from culture, and immunization status. Of 61 950 admissions from January 2008 to December 2012, 2925 (4.7%) had clinical diphtheria; 1194 had been immunized and 1731 were non-immunized. Immunized patients had a milder disease. Culture-positive immunized patients were positive for Corynebacterium other than diphtheriae (COD; n=104) or Corynebacterium diphtheriae (CD; n=23); these patients suffered mild disease and recovered completely. In contrast, culture-positive non-immunized patients were positive for COD (n=11) or CD (n=412). Eighty-one patients (3%) died, 77 of whom were non-immunized; death was usually as a result of myocarditis. Seventy-three percent of deaths were in patients aged diphtheria and its severity and morbidity differ considerably in immunized and non-immunized patients. Disease caused by CD can be deadly, while disease due to COD is mild and responds to treatment. Copyright © 2013 The Authors. Published by Elsevier Ltd.. All rights reserved.

Diphtheria outbreak in Lao People's Democratic Republic, 2012-2013.

Science.gov (United States)

Sein, Carolyn; Tiwari, Tejpratap; Macneil, Adam; Wannemuehler, Kathleen; Soulaphy, Chanthavy; Souliphone, Phouthone; Reyburn, Rita; Ramirez Gonzalez, Alejandro; Watkins, Margaret; Goodson, James L

2016-08-05

Diphtheria is a vaccine-preventable disease. When vaccination coverage and population immunity are low, outbreaks can occur. We investigated a diphtheria outbreak in Lao People's Democratic Republic that occurred during 2012-2013 and highlighted challenges in immunization services delivery to children in the country. We reviewed diphtheria surveillance data from April 1, 2012-May 31, 2013. A diphtheria case was defined as a respiratory illness consisting of pharyngitis, tonsillitis, or laryngitis, and an adherent tonsillar or nasopharyngeal pseudomembrane. To identify potential risk factors for diphtheria, we conducted a retrospective case-control study with two aged-matched neighborhood controls per case-patient in Houaphan Province, using bivariate analysis to calculate matched odds ratio (mOR) with 95% confidence intervals (CI). Reasons for non-vaccination among unvaccinated persons were assessed. Sixty-two clinical cases of diphtheria and 12 diphtheria-related deaths were reported in seven of 17 provinces. Among case-patients, 43 (69%) were diphtheria case-patients from Houaphan province and 79 matched-controls were enrolled. Five (12%) case-patients and 20 (25%) controls had received DTP3 (mOR=0.4, CI=0.1-1.7). No diphtheria toxoid-containing vaccine was received by 20 (48%) case-patients and 38 (46%) controls. Among case-patients and controls with no DTP dose, 43% of case-patients and 40% of controls lacked access to routine immunization services. Suboptimal DTP3 coverage likely caused the outbreak. To prevent continued outbreaks, access to routine immunization services should be strengthened, outreach visits need to be increased, and missed opportunities need to be minimized. In the short term, to rapidly increase population immunity, three rounds of DTP immunization campaign should be completed, targeting children aged 0-14years in affected provinces. Copyright © 2016 Elsevier Ltd. All rights reserved.

METHODS OF CONTROL DIPHTHERIA VACCINE SAFETY

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Isayenko Ye. Yu

2016-12-01

Full Text Available Vaccination success depends not only on the timely coverage of threatened contingents, but also on the quality of vaccines. Every day, the requirements for security guarantees vaccines and their use guarantees of security increases. For the fast, reliable and independent scientific assessment of vaccine safety issues, WHO in 1999 created the Global Advisory Committee on Vaccine Safety. To enhance the capacity of pharmaceutical supervision in relation to vaccines in 2012 it was developed the Global Vaccine Safety Initiative. The main directions of the Global Vaccine Safety programs are considered in this review. It’s noted more strict requirements of Ukrainian pharmaceutical industry to produce public immunization drugs regulated Supplements to the State Pharmacopoeia of Ukraine, in comparison with other countries. This review considered diphtheria vaccine safety monitoring in the process of production according to the recommendations of the World Health Organization (WHO, described a subcutaneous method for determining the specific toxicity of the combined purified toxoid, characterized an intracutaneous method of determining of the presence of diphtheria toxin in each sample of the combined purified toxoid, that additionally used by some manufacturers. The definition of diphtheria toxin in dilutions of purified toxoid is presented. This review considered diphtheria vaccine safety monitoring in the process of production according to the recommendations of the World Health Organization (WHO, described a subcutaneous method for determining the specific toxicity of the combined purified toxoid, characterized an intracutaneous method of determining of the presence of diphtheria toxin in each sample of the combined purified toxoid, that additionally used by some manufacturers. The definition of diphtheria toxin in dilutions of purified toxoid is presented. As methods for determination of diphtheria toxin must be able to detect even a small amount

Prediction of DtxR regulon: Identification of binding sites and operons controlled by Diphtheria toxin repressor in Corynebacterium diphtheriae

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Hasnain Seyed

2004-09-01

Full Text Available Abstract Background The diphtheria toxin repressor, DtxR, of Corynebacterium diphtheriae has been shown to be an iron-activated transcription regulator that controls not only the expression of diphtheria toxin but also of iron uptake genes. This study aims to identify putative binding sites and operons controlled by DtxR to understand the role of DtxR in patho-physiology of Corynebacterium diphtheriae. Result Positional Shannon relative entropy method was used to build the DtxR-binding site recognition profile and the later was used to identify putative regulatory sites of DtxR within C. diphtheriae genome. In addition, DtxR-regulated operons were also identified taking into account the predicted DtxR regulatory sites and genome annotation. Few of the predicted motifs were experimentally validated by electrophoretic mobility shift assay. The analysis identifies motifs upstream to the novel iron-regulated genes that code for Formamidopyrimidine-DNA glycosylase (FpG, an enzyme involved in DNA-repair and starvation inducible DNA-binding protein (Dps which is involved in iron storage and oxidative stress defense. In addition, we have found the DtxR motifs upstream to the genes that code for sortase which catalyzes anchoring of host-interacting proteins to the cell wall of pathogenic bacteria and the proteins of secretory system which could be involved in translocation of various iron-regulated virulence factors including diphtheria toxin. Conclusions We have used an in silico approach to identify the putative binding sites and genes controlled by DtxR in Corynebacterium diphtheriae. Our analysis shows that DtxR could provide a molecular link between Fe+2-induced Fenton's reaction and protection of DNA from oxidative damage. DtxR-regulated Dps prevents lethal combination of Fe+2 and H2O2 and also protects DNA by nonspecific DNA-binding. In addition DtxR could play an important role in host interaction and virulence by regulating the levels of sortase

[Cutaneous diphtheria after a minor injury in Sri Lanka].

Science.gov (United States)

Berg, L; Mechlin, A; Schultz, E S

2016-02-01

Cutaneous dipththeria is an infectious bacterial disease endemic in tropical regions, but rarely diagnosed in Germany. Following travel in Sri Lanka, a 60-year-old German presented to our dermatological clinic with a skin ulcer and extensive erythematous erosive edema of his left foot. Corynebacterium diphtheriae was isolated from a swab of the lesion. There were no clinical signs of toxic diphtheria. The patient was treated with penicillin G and erythromycin, followed by a slow healing of the lesion. The isolated strain could be identified as toxigenic C. diphtheriae mitis. Due to increased travel activity, dermatologists should have uncommon infections like cutaneous diphtheria in mind.

Diphtheria in Lao PDR: Insufficient Coverage or Ineffective Vaccine?

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Nanthavong, Naphavanh; Black, Antony P; Nouanthong, Phonethipsavanh; Souvannaso, Chanthasone; Vilivong, Keooudomphone; Muller, Claude P; Goossens, Sylvie; Quet, Fabrice; Buisson, Yves

2015-01-01

During late 2012 and early 2013 several outbreaks of diphtheria were notified in the North of the Lao People's Democratic Republic. The aim of this study was to determine whether the re-emergence of this vaccine-preventable disease was due to insufficient vaccination coverage or reduction of vaccine effectiveness within the affected regions. A serosurvey was conducted in the Huaphan Province on a cluster sampling of 132 children aged 12-59 months. Serum samples, socio-demographic data, nutritional status and vaccination history were collected when available. Anti-diphtheria and anti-tetanus IgG antibody levels were measured by ELISA. Overall, 63.6% of participants had detectable diphtheria antibodies and 71.2% tetanus antibodies. Factors independently associated with non-vaccination against diphtheria were the distance from the health centre (OR: 6.35 [95% CI: 1.4-28.8], p = 0.01), the Lao Theung ethnicity (OR: 12.2 [95% CI:1,74-85, 4], p = 0.01) and the lack of advice on vaccination given at birth (OR: 9.8 [95% CI: 1.5-63.8], (p = 0.01) while the level of maternal edu-cation was a protective factor (OR: 0.08 [95% CI: 0.008-0.81], p = 0.03). Most respondents claimed financial difficulties as the main reason for non-vaccination. Out of 55 children whose vaccination certificates stated that they were given all 3 doses of diphtheria-containing vaccine, 83.6% had diphtheria antibodies and 92.7% had tetanus antibodies. Furthermore, despite a high prevalence of stunted and underweight children (53% and 25.8%, respectively), the low levels of anti-diphtheria antibodies were not correlated to the nutritional status. Our data highlight a significant deficit in both the vaccination coverage and diphtheria vaccine effectiveness within the Huaphan Province. Technical deficiencies in the methods of storage and distribution of vaccines as well as unreliability of vaccination cards are discussed. Several hypotheses are advanced to explain such a decline in immunity against

CLINICAL FEATURES OF CHILDREN WITH DIPHTHERIA ON SOETOMO HOSPITAL

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Dwiyanti Puspitasari

2017-04-01

Full Text Available Introduction: Diphtheria is an important disease in children that could lead to fatal disease. East Java Province was declared to have Diphtheria outbreak in 2011 with increasing morbidity and mortality, including on Soetomo Hospital. Our paper aimed to describe the profi le of diphtheria cases in children admitted at dr Sutomo Hospital. Method: This descriptive study reviewed all medical records of diphtheritic patients admitted to Dr. Soetomo Hospital, January 2004–December 2010, of concerns were clinical presentations, age, sex, immunizations status, complications, and outcomes. Result: From 148 diphtheria cases, 22.3% were bacteriologically proven; 53.4% were male with sex ratio 1.1:1. The age proportion of ≤ 5 years old, 5- ≤ 10 years old, and > 10 years old were 61.5%, 31.8%, and 6.7%. Fever occurred in 99% cases, sore throat, stridor and bullneck occurred respectively in 62.2%, 39.9%, and 29.7% cases. There were 56.8% severe and 41.9% moderate diphtheria. Subjects were immunized in 84%, but none of them have adequate immunization. Myocarditis, being one of the commonest complications occurred in 11.4% cases and tracheostomy was the second (4.0%. All of the death cases (7.9% were unvaccinated and in severe form. Discussion: The mostly prevalent clinical manifestations in diphtheria children were fever and sore throat. Half of the cases came with severe diphtheria and most cases were inadequately vaccinated. Death occurred in the unvaccinated and severe form.

Purification, crystallization and preliminary crystallographic analysis of the minor pilin FctB from Streptococcus pyogenes

International Nuclear Information System (INIS)

Linke, Christian; Young, Paul G.; Kang, Hae Joo; Proft, Thomas; Baker, Edward N.

2010-01-01

The minor pilin FctB from S. pyogenes strain 90/306S was expressed in E. coli, purified and crystallized. The hexagonal FctB crystals diffracted to 2.9 Å resolution. The minor pilin FctB is an integral part of the pilus assembly expressed by Streptococcus pyogenes. Since it is located at the cell wall, it can be hypothesized that it functions as a cell-wall anchor for the streptococcal pilus. In order to elucidate its structure, the genes for FctB from the S. pyogenes strains 90/306S and SF370 were cloned for overexpression in Escherichia coli. FctB from strain 90/306S was crystallized by the sitting-drop vapour-diffusion method using sodium citrate as a precipitant. The hexagonal FctB crystals belonged to space group P6 1 or P6 5 , with unit-cell parameters a = b = 95.15, c = 100.25 Å, and diffracted to 2.9 Å resolution

Anti-diphtheria immunity in Nigerian mothers and their newborns.

Science.gov (United States)

Cummings, Henry; Sadoh, Ayebo Evawere; Oviawe, Osawaru; Sadoh, Wilson Ehidiamen

2014-05-30

Immunity to diphtheria has been noted to wane with age such that previous studies have shown that a significant proportion of females with characteristics comparable to those of Nigerian women of reproductive age have inadequate levels of immunity to diphtheria. Thus, it is envisaged that Nigerian newborns may inherit inadequate levels of immunity to diphtheria from their mothers. Cord blood and peripheral maternal blood samples were collected from 231 mother-infant pairs at delivery. Anti-diphtheria antibody titres were assayed using Enzyme-linked immunosorbent assay (ELISA) technique. Recruited babies were those born at term with normal birth weight. As much as 29.9% of both mothers and their babies had no protection (antibody titrediphtheria. Ninety (39.0% CI 33%,45%) mothers and 107 (46.3% CI 40%,52%) babies were inadequately protected (antibody titrediphtheria. The difference in the geometric mean antibody titres of mothers and babies was statistically significant (pdiphtheria. Vaccination of parturient women with booster doses of diphtheria toxoid vaccine is recommended. Copyright © 2014 Elsevier Ltd. All rights reserved.

Baseline immunity to diphtheria and immunologic response after booster vaccination with reduced diphtheria and tetanus toxoid vaccine in Thai health care workers.

Science.gov (United States)

Wiboonchutikul, Surasak; Manosuthi, Weerawat; Sangsajja, Chariya; Thientong, Varaporn; Likanonsakul, Sirirat; Srisopha, Somkid; Termvises, Patamavadee; Rujitip, Jitlada; Loiusirirotchanakul, Suda; Puthavathana, Pilaipan

2014-07-01

A prospective study to evaluate immune status against diphtheria and immunologic response after tetanus-diphtheria (Td) booster vaccination was conducted in 250 Thai health care workers (HCWs). A protective antibody was found in 89.2% of the HCWs (95% confidence interval [CI], 83.3%-91.5%) before receipt of the Td booster vaccination, compared with 97.2% (95% CI, 95.1%-99.3%) after receipt of the first dose of booster (P diphtheria increased from 0.39 IU/mL (95% CI, 0.35-0.44 IU/mL) before the Td booster vaccination to 1.20 IU/mL (95% CI, 1.12-1.29 IU/mL) after the vaccination (P diphtheria, which still circulates in Thailand. Copyright © 2014 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Mosby, Inc. All rights reserved.

Tetanus, Diphtheria, and Pertussis Vaccines: MedlinePlus Health Topic

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... Know (Centers for Disease Control and Prevention) - PDF Topic Image MedlinePlus Email Updates Get Tetanus, Diphtheria, and ... updates by email What's this? GO Related Health Topics Childhood Immunization Diphtheria Immunization Tetanus Whooping Cough National ...

Immunity to tetanus and diphtheria in the UK in 2009.

Science.gov (United States)

Wagner, Karen S; White, Joanne M; Andrews, Nick J; Borrow, Ray; Stanford, Elaine; Newton, Emma; Pebody, Richard G

2012-11-19

This study aimed to estimate the immunity of the UK population to tetanus and diphtheria, including the potential impact of new glycoconjugatate vaccines, and the addition of diphtheria to the school leaver booster in 1994. Residual sera (n=2697) collected in England in 2009/10 were selected from 18 age groups and tested for tetanus and diphtheria antibody. Results were standardised by testing a panel of sera (n=150) to enable comparison with a previously (1996) published serosurvey. Data were then standardised to the UK population. In 2009, 83% of the UK population were protected (≥0.1 IU/mL) against tetanus compared to 76% in 1996 (p=0.079), and 75% had at least basic protection against diphtheria (≥0.01 IU/mL) in 2009 compared to 60% in 1996 (pdiphtheria. Higher diphtheria immunity was observed in those aged 16-34 years in 2009 compared to 1996 (geometric mean concentration [GMC] 0.15 IU/mL vs. 0.03 IU/mL, pdiphtheria in 2009 were 29% susceptible), 45-69 years (>20% susceptible) and 70+ years (>32% susceptible). Low immunity was observed in those aged 10-11 years (>19% susceptible), between the scheduled preschool and school leaver booster administration. The current schedule appears to induce protective levels; increases in the proportions protected/GMCs were observed for the ages receiving vaccinations according to UK policy. Glycoconjugate vaccines appear to have increased immunity, in particular for diphtheria, in preschool age groups. Diphtheria immunity in teenagers and young adults has increased as a result of the addition of diphtheria to the school leaver booster. However, currently older adults remain susceptible, without any further opportunities for immunisations planned according to the present schedule. Copyright © 2012 Elsevier Ltd. All rights reserved.

Shaft Boring Machine: A method of mechanized vertical shaft excavation

International Nuclear Information System (INIS)

Goodell, T.M.

1991-01-01

The Shaft Boring Machine (SBM) is a vertical application of proven rock boring technology. The machine applies a rotating cutter wheel with disk cutters for shaft excavation. The wheel is thrust against the rock by hydraulic cylinders and slews about the shaft bottom as it rotates. Cuttings are removed by a clam shell device similar to conventional shaft mucking and the muck is hoisted by buckets. The entire machine moves down (and up) the shaft through the use of a system of grippers thrust against the shaft wall. These grippers and their associated cylinders also provide the means to maintain verticality and stability of the machine. The machine applies the same principles as tunnel boring machines but in a vertical mode. Other shaft construction activities such as rock bolting, utility installation and shaft concrete lining can be accomplished concurrent with shaft boring. The method is comparable in cost to conventional sinking to a depth of about 460 meters (1500 feet) beyond which the SBM has a clear host advantage. The SBM has a greater advantage in productivity in that it can excavate significantly faster than drill and blast methods

International External Quality Assurance for Laboratory Diagnosis of Diphtheria ▿

Science.gov (United States)

Neal, S. E.; Efstratiou, A.

2009-01-01

The diphtheria surveillance network (DIPNET) encompassing National Diphtheria Reference Centers from 25 European countries is a Dedicated Surveillance Network recognized by the European Commission. A key DIPNET objective is the quality assessment of microbiological procedures for diphtheria across the European Union and beyond. A detailed questionnaire on the level of reference laboratory services and an external quality assessment (EQA) panel comprising six simulated throat specimens were sent to 34 centers. Twenty-three centers are designated National Diphtheria Reference Centers, with the laboratory in the United Kingdom being the only WHO Collaborating Centre. A variety of screening and identification tests were used, including the cysteinase test (20/34 centers), pyrazinamidase test (17/34 centers), and commercial kits (25/34 centers). The classic Elek test for toxigenicity testing is mostly used (28/34 centers), with variations in serum sources and antitoxin concentrations. Many laboratories reported problems obtaining Elek reagents or media. Only six centers produced acceptable results for all six specimens. Overall, 21% of identification and 13% of toxigenicity reports were unacceptable. Many centers could not isolate the target organism, and most found difficulties with the specimens that contained Corynebacterium striatum as a commensal contaminant. Nineteen centers generated either false-positive or negative toxigenic results, which may have caused inappropriate medical management. The discrepancies in this diphtheria diagnostics EQA alarmingly reflect the urgent need to improve laboratory performance in diphtheria diagnostics in Europe, standardize feasible and robust microbiological methods, and build awareness among public health authorities. Therefore, DIPNET recommends that regular workshops and EQA distributions for diphtheria diagnostics should be supported and maintained. PMID:19828749

Diphtheria in the Dominican Republic: reduction of cases following a large outbreak.

Science.gov (United States)

Garib, Zacarías; Danovaro-Holliday, M Carolina; Tavarez, Yira; Leal, Irene; Pedreira, Cristina

2015-10-01

To describe the most recent outbreak of diphtheria in the Dominican Republic and the disease's occurrence and vaccination coverage in 2004-2013. Clinical data of diphtheria cases that occurred in 2004 and that met the study's case definition were reviewed along with socioeconomic and epidemiological information from the cases' families. Univariate and multivariate analyses were performed to assess risk factors for fatal diphtheria. Routine surveillance and vaccination coverage data are presented. From January 2004-April 2005, a total of 145 diphtheria cases were reported; 80 (66%) of the 122 cases reported in 2004 met the case definition; 26 were fatal (case-fatality rate: 32.5%). Incidence was highest in the group 1-4 years of age at 5.3 per 100 000; 62.5% were male. Of the 80 cases, 61 (76%) where hospitalized in Hospital A, 17 in Hospital B, and 2 in two other hospitals. Earlier onset (first half of 2004), birth order, and tracheotomy were associated with fatal diphtheria (P diphtheria incidence was 4.91 cases/1 million people in 2000-2003, climbed to 8.8 cases per million in 2004-2005, and dropped to 0.38 in 2006-2014; no diphtheria cases have been reported since 2011. DTP3 vaccination coverage ranged from 72%-81% in 2000-2004 and from 81%-89% in 2005-2013. The 2004-2005 diphtheria outbreak in the Dominican Republic resulted in important and avoidable morbidity and mortality. Annual cases declined and no cases have been reported in recent years. Maintaining high vaccination coverage and diligent surveillance are crucial to preventing diphtheria outbreaks and controlling the disease.

Using combined system of shaft guides for buckets during shaft deepening

Energy Technology Data Exchange (ETDEWEB)

Durov, E.M.; Ivenskii, N.S.; Alekhin, P.I.

1981-06-01

This paper discusses a system of shaft guides used in the Krasnopol'evsk underground coal mine. The existing skip shaft 514 m deep is deepened to a depth of 700 m. Shaft design is adapted to a system of two pairs of skips, however, only one pair of skips is in operation and the other has been removed. The free space can be used to remove rock material from shaft bottom. It is noted that a system of buckets moving along elastic shaft guides made of rope or along rigid shaft guides can be used. Both solutions have numerous advantages. If rope guides are used time consuming installation of shaft guides is unnecessary in the zone close to the bottom. If rigid guides are used capacity of the bucket can be significantly increased. A system which combines advantages of both solutions is used: in the lower part of the shaft being deepened, buckets are guided by rope, and in the upper zone in which rigid shaft guides have been installed the bucket moves along rigid guides and rope guides simultaneously. Design of the element guiding the bucket is shown in two diagrams. It is noted that using the combined system of shaft guides increases capacity of the hoisting system by 1.5 times.

Microbe Profile: Corynebacterium diphtheriae - an old foe always ready to seize opportunity.

Science.gov (United States)

Hoskisson, Paul A

2018-02-21

Corynebacterium diphtheriae is a globally important Gram-positive aerobic Actinobacterium capable of causing the toxin-mediated disease, diphtheria. Diphtheria was a major cause of childhood mortality prior to the introduction of the toxoid vaccine, yet it is capable of rapid resurgence following the breakdown of healthcare provision, vaccination or displacement of people. The mechanism and treatment of toxin-mediated disease is well understood, however there are key gaps in our knowledge on the basic biology of C. diphtheriae particularly relating to host colonisation, the nature of asymptomatic carriage, population genomics and host adaptation.

A Case-control Study of Diphtheria in the High Incidence City of Hyderabad, India.

Science.gov (United States)

Allam, Ramesh Reddy; Uthappa, Chengappa Kechamada; Duerst, Rebecca; Sorley, Evan; Udaragudi, Prasada Rao; Kampa, Shankar; Dworkin, Mark S

2016-03-01

India accounts for approximately 72% of reported diphtheria cases globally, the majority of which occur in the state of Andhra Pradesh. The aim of this study is to better understand lack of knowledge on diphtheria vaccination and to determine factors associated with diphtheria and low knowledge and negative attitudes. We performed a 1:1 case-control study of hospitalized diphtheria cases in Hyderabad. Eligible case patients were 10 years of age or older, resided within the city of Hyderabad and were diagnosed with diphtheria per the case definition. Patients admitted to the hospital for nonrespiratory communicable diseases and residing in the same geographic region as that of cases were eligible for enrolment as controls : There were no statistical differences in disease outcome by gender, education, economic status and mean room per person sleeping in the house in case and control subjects. Not having heard of diphtheria (adjusted odds ratio: 3.56; 95% confidence intervals: 1.58-8.04] and not believing that vaccines can prevent people from getting diseases (adjusted odds ratio: 3.99; 95% confidence intervals: 1.18-13.45) remained significantly associated with diphtheria on multivariate analysis. To reduce the burden of diphtheria in India, further efforts to educate the public about diphtheria should be considered.

Diphtheria in the Dominican Republic: reduction of cases following a large outbreak

Directory of Open Access Journals (Sweden)

Zacarías Garib

Full Text Available OBJECTIVE: To describe the most recent outbreak of diphtheria in the Dominican Republic and the disease's occurrence and vaccination coverage in 2004-2013. METHODS: Clinical data of diphtheria cases that occurred in 2004 and that met the study's case definition were reviewed along with socioeconomic and epidemiological information from the cases' families. Univariate and multivariate analyses were performed to assess risk factors for fatal diphtheria. Routine surveillance and vaccination coverage data are presented. RESULTS: From January 2004-April 2005, a total of 145 diphtheria cases were reported; 80 (66% of the 122 cases reported in 2004 met the case definition; 26 were fatal (case-fatality rate: 32.5%. Incidence was highest in the group 1-4 years of age at 5.3 per 100 000; 62.5% were male. Of the 80 cases, 61 (76% where hospitalized in Hospital A, 17 in Hospital B, and 2 in two other hospitals. Earlier onset (first half of 2004, birth order, and tracheotomy were associated with fatal diphtheria (P < 0.05; cases in Hospital A were also more likely to be fatal (P = 0.066. The average annual diphtheria incidence was 4.91 cases/1 million people in 2000-2003, climbed to 8.8 cases per million in 2004-2005, and dropped to 0.38 in 2006-2014; no diphtheria cases have been reported since 2011. DTP3 vaccination coverage ranged from 72%-81% in 2000-2004 and from 81%-89% in 2005-2013. CONCLUSIONS: The 2004-2005 diphtheria outbreak in the Dominican Republic resulted in important and avoidable morbidity and mortality. Annual cases declined and no cases have been reported in recent years. Maintaining high vaccination coverage and diligent surveillance are crucial to preventing diphtheria outbreaks and controlling the disease.

Primary vaccination of adults with reduced antigen-content diphtheria-tetanus-acellular pertussis or dTpa-inactivated poliovirus vaccines compared to diphtheria-tetanus-toxoid vaccines.

NARCIS (Netherlands)

Theeten, H.; Rumke, H.C.; Hoppener, F.J.; Vilatimo, R.; Narejos, S.; Damme, P. van; Hoet, B.

2007-01-01

OBJECTIVE: To evaluate immunogenicity and reactogenicity of primary vaccination with reduced-antigen-content diphtheria-tetanus-acellular pertussis (dTpa) or dTpa-inactivated poliovirus (dTpa-IPV) vaccine compared to diphtheria-tetanus-toxoid vaccines (Td) in adults > or = 40 years of age without

Diphtheria outbreak in Maranhão, Brazil: microbiological, clinical and epidemiological aspects.

Science.gov (United States)

Santos, L S; Sant'anna, L O; Ramos, J N; Ladeira, E M; Stavracakis-Peixoto, R; Borges, L L G; Santos, C S; Napoleão, F; Camello, T C F; Pereira, G A; Hirata, R; Vieira, V V; Cosme, L M S S; Sabbadini, P S; Mattos-Guaraldi, A L

2015-03-01

We describe microbiological, clinical and epidemiological aspects of a diphtheria outbreak that occurred in Maranhão, Brazil. The majority of the 27 confirmed cases occurred in partially (n = 16) or completely (n = 10) immunized children (n = 26). Clinical signs and characteristic symptoms of diphtheria such as cervical lymphadenopathy and pseudomembrane formation were absent in 48% and 7% of the cases, respectively. Complications such as paralysis of lower limbs were observed. Three cases resulted in death, two of them in completely immunized children. Microbiological analysis identified the isolates as Corynebacterium diphtheriae biovar intermedius with a predominant PFGE type. Most of them were toxigenic and some showed a decrease in penicillin G susceptibility. In conclusion, diphtheria remains endemic in Brazil. Health professionals need to be aware of the possibility of atypical cases of C. diphtheriae infection, including pharyngitis without pseudomembrane formation.

Shaft/shaft-seal interface characteristics of a multiple disk centrifugal blood pump.

Science.gov (United States)

Manning, K B; Miller, G E

1999-06-01

A multiple disk centrifugal pump (MDCP) is under investigation as a potential left ventricular assist device. As is the case with most shaft driven pumps, leakage problems around the shaft/shaft seal interface are of major interest. If leakage were to occur during or after implantation, potential events such as blood loss, clotting, blood damage, and/or infections might result in adverse effects for the patient. Because these effects could be quite disastrous, potential shaft and shaft seal materials have been investigated to determine the most appropriate course to limit these effects. Teflon and nylon shaft seals were analyzed as potential candidates along with a stainless steel shaft and a Melonite coated shaft. The materials and shafts were evaluated under various time durations (15, 30, 45, and 60 min), motor speeds (800, 1,000, 1,200, and 1,400 rpm), and outer diameters (1/2 and 3/4 inches). The motor speed and geometrical configurations were typical for the MDCP under normal physiologic conditions. An air and water study was conducted to analyze the inner diameter wear, the inner temperature values, and the outer temperature values. Statistical comparisons were computed for the shaft seal materials, the shafts, and the outer diameters along with the inner and outer temperatures. The conclusions made from the results indicate that both the tested shaft seal materials and shaft materials are not ideal candidates to be used for the MDCP. Teflon experienced a significant amount of wear in air and water studies. Nylon did experience little wear, but heat generation was an evident problem. A water study on nylon was not conducted because of its molecular structure.

Seroprevalence of diphtheria toxoid IgG antibodies in children, adolescents and adults in Poland.

Science.gov (United States)

Zasada, Aleksandra A; Rastawicki, Waldemar; Rokosz, Natalia; Jagielski, Marek

2013-11-19

Recommendations for diphtheria immunization are to apply an effective primary immunization in infancy and to maintain immunity throughout life. Immunity against diphtheria depends primarily on antibody to the diphtheria toxin. This study evaluated the seroprevalence of IgG diphtheria antitoxin in sera of healthy children, adolescents and adults in Poland. A total of 1387 serum samples collected between 2010 and 2012 from individuals with ages ranging from 1 month to 85 years were investigated. Antibody concentrations were measured with an enzyme-linked immunosorbent assay (Anti-Diphtheria Toxoid ELISA IgG, Euroimmun, Germany). The results showed that among 1387 individuals examined, 547 (39.4%) had anti-diphtheria toxoid IgG antibody levels below 0.1 IU/ml (36.9% ≤ 18 years and 40.5% >18 years old, respectively). The 212 (50.8%) children and 542 (55.9%) adults showed only basic protection (0.1-1.0 IU/ml) and need immediate booster. High levels of anti-diphtheria toxoid IgG antibodies (>1.0 IU/ml) were found more often in children and adolescent (12.2%) than in adults (3.6%) and this was statistically significant (P 60 years old. Characteristically, in individuals > 40 years old high levels of anti-diphtheria toxoid IgG antibodies (>1.0 IU/ml) were not seen. There were no statistically significant differences in results in relation to gender. The present study showed inadequate immunity levels to diphtheria amongst the Polish population, especially in adults > 40 years old and children ≤ 2 years old. To prevent reemergence of diphtheria an information campaign reminding people about recommendations concerning diphtheria booster vaccination in adults should be conducted. Moreover, the immunogenicity of the DTP vaccine used in Poland should be verified.

Structure of the CFA/III major pilin subunit CofA from human enterotoxigenic Escherichia coli determined at 0.90 Å resolution by sulfur-SAD phasing.

Science.gov (United States)

Fukakusa, Shunsuke; Kawahara, Kazuki; Nakamura, Shota; Iwashita, Takaki; Baba, Seiki; Nishimura, Mitsuhiro; Kobayashi, Yuji; Honda, Takeshi; Iida, Tetsuya; Taniguchi, Tooru; Ohkubo, Tadayasu

2012-10-01

CofA, a major pilin subunit of colonization factor antigen III (CFA/III), forms pili that mediate small-intestinal colonization by enterotoxigenic Escherichia coli (ETEC). In this study, the crystal structure of an N-terminally truncated version of CofA was determined by single-wavelength anomalous diffraction (SAD) phasing using five sulfurs in the protein. Given the counterbalance between anomalous signal strength and the undesired X-ray absorption of the solvent, diffraction data were collected at 1.5 Å resolution using synchrotron radiation. These data were sufficient to elucidate the sulfur substructure at 1.38 Å resolution. The low solvent content (29%) of the crystal necessitated that density modification be performed with an additional 0.9 Å resolution data set to reduce the phase error caused by the small sulfur anomalous signal. The CofA structure showed the αβ-fold typical of type IVb pilins and showed high structural homology to that of TcpA for toxin-coregulated pili of Vibrio cholerae, including spatial distribution of key residues critical for pilin self-assembly. A pilus-filament model of CofA was built by computational docking and molecular-dynamics simulation using the previously reported filament model of TcpA as a structural template. This model revealed that the CofA filament surface was highly negatively charged and that a 23-residue-long loop between the α1 and α2 helices filled the gap between the pilin subunits. These characteristics could provide a unique binding epitope for the CFA/III pili of ETEC compared with other type IVb pili.

Isopeptide bonds of the major pilin protein BcpA influence pilus structure and bundle formation on the surface of Bacillus cereus

Energy Technology Data Exchange (ETDEWEB)

Hendrickx, Antoni P.A.; Poor, Catherine B.; Jureller, Justin E.; Budzik, Jonathan M.; He, Chuan; Schneewind, Olaf (UC)

2012-09-05

Bacillus cereus strains elaborate pili on their surface using a mechanism of sortase-mediated cross-linking of major and minor pilus components. Here we used a combination of electron microscopy and atomic force microscopy to visualize these structures. Pili occur as single, double or higher order assemblies of filaments formed from monomers of the major pilin, BcpA, capped by the minor pilin, BcpB. Previous studies demonstrated that within assembled pili, four domains of BcpA -- CNA{sub 1}, CNA{sub 2}, XNA and CNA{sub 3} -- each acquire intramolecular lysine-asparagine isopeptide bonds formed via catalytic glutamic acid or aspartic acid residues. Here we showed that mutants unable to form the intramolecular isopeptide bonds in the CNA2 or CNA3 domains retain the ability to form pilus bundles. A mutant lacking the CNA{sub 1} isopeptide bond assembled deformed pilin subunits that failed to associate as bundles. X-ray crystallography revealed that the BcpA variant Asp{sup 312}Ala, lacking an aspartyl catalyst, did not generate the isopeptide bond within the jelly-roll structure of XNA. The Asp{sup 312}Ala mutant was also unable to form bundles and promoted the assembly of deformed pili. Thus, structural integrity of the CNA{sub 1} and XNA domains are determinants for the association of pili into higher order bundle structures and determine native pilus structure.

[THE EFFECTIVENESS OF VACCINATION AGAINST DIPHTHERIA IN THE VORONEZH REGION].

Science.gov (United States)

Mamchik, N P; Gabbasova, N V; Sitnik, T N; Borisova, L S

2015-01-01

The purpose of the study was the assessment of the effectiveness of vaccination against diphtheria in the Voronezh region over the epidemic period of 1993-1997 and epidemiological welfare during 2010-2014. of the study: data of the official statistical reporting--forms number 1, 6, the serum level of antitoxic antibodies to diphtheria in 19319 healthy individuals were analyzed with the aid of epidemiological (descriptive and evaluative), immunological and statistical methods. During the epidemic rise of diphtheria (1993-1997) 75% of cases were amounted to the adult population of the Voronezh region, half of them--were not immunized against diphtheria. In 1993 there was begun mass vaccination of adult population, immunization coverage by 1998 reached 95%. According to seromonitoring data the share of seronegatives to diphtheria among cases examined during the period of 1995-2000 accountedfrom 11.9 to 24.9%. During the period of sporadic morbidity (1998-2007 years) among patients the 80% of cases have been vaccinated with an interval from the last inoculation of 3-5 years, which casts doubt on the effectiveness of vaccines. Since 2008 the incidence of diphtheria in the Voronezh region was not recorded. Against the background of 98% coverage of vaccination of the total population, the share of seronegatives for the last 5 years have decreased by 2.5 times and in 2014 reached the required performance. Documented inoculation indices fail to reflect the level of the actual protection against infection. In the conditions of the absence of the morbidity only serological monitoring is an objective criterion of the protectability of the population from infection.

Immunochromatographic Strip Test for Rapid Detection of Diphtheria Toxin: Description and Multicenter Evaluation in Areas of Low and High Prevalence of Diphtheria

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Engler, K. H.; Efstratiou, A.; Norn, D.; Kozlov, R. S.; Selga, I.; Glushkevich, T. G.; Tam, M.; Melnikov, V. G.; Mazurova, I. K.; Kim, V. E.; Tseneva, G. Y.; Titov, L. P.; George, R. C.

2002-01-01

An immunochromatographic strip (ICS) test was developed for the detection of diphtheria toxin by using an equine polyclonal antibody as the capture antibody and colloidal gold-labeled monoclonal antibodies specific for fragment A of the diphtheria toxin molecule as the detection antibody. The ICS test has been fully optimized for the detection of toxin from bacterial cultures; the limit of detection was approximately 0.5 ng of diphtheria toxin per ml within 10 min. In a comparative study with 915 pure clinical isolates of Corynebacterium spp., the results of the ICS test were in complete agreement with those of the conventional Elek test. The ICS test was also evaluated for its ability to detect toxigenicity from clinical specimens (throat swabs) in two field studies conducted within areas of the former USSR where diphtheria is epidemic. Eight hundred fifty throat swabs were examined by conventional culture and by use of directly inoculated broth cultures for the ICS test. The results showed 99% concordance (848 of 850 specimens), and the sensitivity and specificity of the ICS test were 98% (95% confidence interval, 91 to 99%) and 99% (95% confidence interval, 99 to 100%), respectively. PMID:11773096

Seroprevalence study of anti diphtheria antibodies in two age-groups of Romanian adults.

Science.gov (United States)

Dragomirescu, Cristiana Cerasella; Coldea, Ileana-Luminiţa; Ilie, Anamaria; Stănescu, Aurora; Ungureanu, Vasilica; Popa, Mircea Ioan

2014-01-01

Diphtheria represents a serious infectious disease with high epidemic potential. It is a vaccine preventable disease (a minimum vaccine coverage of 95% for children of 1 year and 90% in adults could prevent the disease). Diphtheria vaccination is included in the National Immunization Program (NIP). Complete vaccination for children consists in DTaP (diphtheria toxoid, tetanus toxoid and acellular pertussis vaccine) vaccine administration from the age of 2 months until 4 years and dT vaccine (tetanus toxoid and a reduced dose of diphtheria toxoid) at 14 years old. The aim of this paper was to highlight the protection against diphtheria of an age segment of the Romanian adult population (20 to 39 years old) using a seroprevalence study. The Romanian subjects were selected from two age groups: 20-29 years (n = 219) and 30-39 years (n = 229), representative for all counties of Romania. The commercial kit Anti-Diphtheria Toxoid ELISA (IgG) (EUROIMMUN) was used to detect the antibodies of IgG class against diphtheria toxoid in the sera obtained from our subjects. We detected a 56.6% rate of positive sera (> 0.1 IU/ml--protection level) for the 20-29 age group and 31.7% positivity for the 30-39 age group. These data show a low protection level against diphtheria of the Romanian adult population, which decreases with age. The serologic data on preventable vaccine diseases are useful in order to evaluate the success of the immunization programs.

Toxigenic cutaneous diphtheria in a returned traveller.

Science.gov (United States)

Abdul Rahim, Nur R; Koehler, Ann P; Shaw, Doug D; Graham, Caitlin R

2014-12-31

Diphtheria is rarely reported in Australia. A case of cutaneous diphtheria was reported to the South Australian Department for Health and Ageing in April 2013 in an Australian-born 18-year-old female following travel in India. The case presented with a skin ulcer on her toe. Toxigenic Corynebacterium diphtheriae was isolated from a swab of the lesion. The case was treated with antibiotics. The public health response included infection control advice, assessing the case and household contacts for organism carriage and providing antimicrobial chemoprophylaxis to contacts. Although cutaneous diphtheria is not included as part of the Australian communicable disease surveillance case definition, this may be an oversight as international evidence demonstrates that it is a source of organism transmission and can potentially result in outbreaks among susceptible populations. This formed the rationale for the public health response to this particular case. The protocol for the public health management of diphtheria in South Australia has since been revised to include cutaneous lesions caused by the toxigenic strain of the organism as part of the surveillance case definition. This work is copyright. You may download, display, print and reproduce the whole or part of this work in unaltered form for your own personal use or, if you are part of an organisation, for internal use within your organisation, but only if you or your organisation do not use the reproduction for any commercial purpose and retain this copyright notice and all disclaimer notices as part of that reproduction. Apart from rights to use as permitted by the Copyright Act 1968 or allowed by this copyright notice, all other rights are reserved and you are not allowed to reproduce the whole or any part of this work in any way (electronic or otherwise) without first being given the specific written permission from the Commonwealth to do so. Requests and inquiries concerning reproduction and rights are to be sent

Diphtheria and the Vaccine (Shot) to Prevent It: Information for Parents

Science.gov (United States)

... PARENTS | DISEASES and the VACCINES THAT PREVENT THEM | Diphtheria and the Vaccine (Shot) to Prevent It Last ... April 2017 The best way to protect against diphtheria is by getting the diphther ia-tet anus- ...

Tdap Vaccine (Tetanus, Diphtheria and Pertussis): What You Need to Know

Science.gov (United States)

... Tdap Vaccine What You Need to Know (Tetanus, Diphtheria and Pertussis) Many Vaccine Information Statements are available ... immunize. org/ vis 1 Why get vaccinated? Tetanus, diphtheria and pertussis are very serious diseases. Tdap vaccine ...

INTERACTION OF RECOMBINANT DIPHTHERIA TOXOIDS WITH CELLULAR RECEPTORS in vitro

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K. Yu. Manoilov

2016-06-01

Full Text Available The aim of the research was to compare in vitro characteristics of reception of the natural diphtheria toxin — DT and its nontoxic recombinant analogs — toxoids. For assessing ligand-receptor interaction the method of immunoenzyme analysis and ELISA was used, where the bonding layer recombinant analogues of diphtheria toxin cell receptor HB-EGF from sensitive and resistant to the toxin of the organisms were served. According to the results of ELISA the natural diphtheria toxin, in contrast to recombinant toxoids — CRM197, and B subunit, interacted with mouse HB-EGF with a very low affinity. While human HB-EGF with an equally high affinity connected as toxoids as native diphtheria toxin. Therefore, the analyzed recombinant analogs of toxin obtained in E. coli cells did not reproduce in full measure the receptor specificity of the natural toxin, which should be considered in the case of using these proteins as biotech products.

Level of Immunological Protection against Diphtheria in the Population of Dnipropetrovsk Region

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G.O. Revenko

2016-11-01

Full Text Available Background. Diphtheria — an infectious disease, the spread of which is limited by population post-vaccination immunity. In the system of epidemiological surveillance of diphtheria, for objective assessment and prediction of epidemic situation in low intensity of epidemic process of this infection, the serological control of immunity is crucial. The objective: prognosis of epidemic situation in terms of the risk of diphtheria outbreaks in Ukraine by the detection of population post-vaccination immunity against diphtheria in Dnipropetrovsk region and evaluation of the effectiveness of widespread vaccination. Materials and methods. 483 people aged 0 to 78 years were examined in total in 2015 (children — 234, adults — 249 persons. Evaluation of specific immunity against diphtheria was carried on with the following criteria: those with levels of antibodies 0.015–0.06 IU/mL should be considered as relatively protected; 0.1–0.5 IU/ml — with average level of protection; > 1.0 IU/ml — with high level of protection. Protective antibody titer should be considered the concentration of not less than 0.1 IU/ml. Results. It was established that the level of protective immunity against diphtheria was 69.5 %. Reliable level of immunological protection of children was 70.9 %, among adults — 68.3 %. None age group had sufficient threshold level of antitoxic immunity required to stop the transmission of diphtheria pathogen. Percentage of protected persons decreased with increasing age of people (both in children and adults. This situation may create conditions for outbreaks of diphtheria in Dnipropetrovsk region. Conclusions. The presented results indicate the importance of maintaining protective antibody levels through widespread immunization of children, as well as booster vaccination among adolescents and adults that will exclude the category of susceptible layer of the population.

Shaft adjuster

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Harry, Herbert H.

1989-01-01

Apparatus and method for the adjustment and alignment of shafts in high power devices. A plurality of adjacent rotatable angled cylinders are positioned between a base and the shaft to be aligned which when rotated introduce an axial offset. The apparatus is electrically conductive and constructed of a structurally rigid material. The angled cylinders allow the shaft such as the center conductor in a pulse line machine to be offset in any desired alignment position within the range of the apparatus.

Resurgence of diphtheria in rural areas of North Karnataka, India.

Science.gov (United States)

Parande, Mahantesh V; Roy, Subarna; Mantur, B G; Parande, Aisha M; Shinde, Rupali S

2017-01-01

A diphtheria outbreak was identified from Vijayapura (formerly Bijapur) district in the South Indian state of Karnataka in 2011. There was a surge in the number of throat swab samples received under the Integrated Disease Surveillance Programme (IDSP) in North Karnataka since then. A microbiological study was undertaken to generate information on the status of resurgence of the disease in the region. Throat swabs from 432 suspected cases of diphtheria during 2012-2015 were obtained from government hospitals and primary health centres of 8 districts in North Karnataka and were processed for the culture and identification of Corynebacterium diphtheriae. Polymerase chain reaction for the presence of toxin gene (toxA and toxB) was carried out on the isolates. Antibiotic sensitivity tests were performed on the isolates with a panel of 14 antibiotics. Thirty-eight (8.79%) out of 432 samples yielded C. diphtheriae on culture. All isolates possessed the diphtheria toxin gene. Out of the 38 confirmed cases, whereas 21 (55.26%) were between 1 and 5 years of age, 14 (36.84%) were aged between 5 and 10 years. Male children were three times more than females in confirmed cases. No information was available on the immunisation status of the cases. Emergence of resistance to penicillin was found with minimum inhibitory concentration reaching up to 6.00 μg/ml. Our study identified an upsurge in cases of diphtheria in North Karnataka, particularly in Vijayapura District, and to the best of our knowledge, reports the emergence of penicillin resistance for the first time in India. The study calls for enhanced surveillance for the disease, making antidiphtheritic serum available in key hospitals in the region and serves to provide a baseline for future assessment of the impact of the recently launched 'Mission Indradhanush' programme in strengthening Universal Immunisation Programme (UIP).

Structural perturbation of diphtheria toxoid upon adsorption to aluminium hydroxide adjuvant

NARCIS (Netherlands)

Regnier, M.; Metz, B.; Tilstra, W.; Hendriksen, C.; Jiskoot, W.; Norde, W.; Kersten, G.

2012-01-01

Aluminium-containing adjuvants are often used to enhance the potency of vaccines. In the present work we studied whether adsorption of diphtheria toxoid to colloidal aluminium hydroxide induces conformational changes of the antigen. Diphtheria toxoid has a high affinity for the aluminium hydroxide

Immunogenicity and safety of one dose of diphtheria, tetanus, acellular pertussis and poliomyelitis vaccine (Repevax®) followed by two doses of diphtheria, tetanus and poliomyelitis vaccine (Revaxis®) in adults aged ≥ 40 years not receiving a diphtheria- and tetanus-containing vaccination in the last 20 years.

Science.gov (United States)

Dominicus, Rolf; Galtier, Florence; Richard, Patrick; Baudin, Martine

2014-06-30

The immunogenicity and safety of one dose of Tdap-IPV (tetanus, diphtheria, acellular pertussis and inactivated poliomyelitis vaccine) and two doses of Td-IPV (tetanus, diphtheria and inactivated poliomyelitis vaccine) were assessed in adults who had not received a diphtheria- and tetanus-containing vaccine in the last 20 years. This open-label, multicentre study was conducted in adults aged ≥ 40 years with no diphtheria- and tetanus-containing vaccine in the last 20 years. Participants received one dose of Tdap-IPV followed by two doses of Td-IPV (0, 1, 6 month schedule). Primary immunogenicity objectives: to demonstrate acceptable seroprotection rates (percentage of participants with antibody titre above threshold) post-dose 3 for diphtheria (≥ 0.1IU/mL by seroneutralization assay [SNA]); tetanus (≥ 0.1IU/mL by enzyme-linked immunosorbent assay [ELISA]); and poliomyelitis (≥ 8 1/dil by SNA); and to evaluate the percentage of participants with an antibody concentration ≥ 5EU/mL (by ELISA) for pertussis antigens post-dose 1. Seroprotection rates were acceptable if the lower limit of the 95% confidence interval (CI) was >95%. Percentage of participants with basic clinical immunity against diphtheria (≥ 0.01IU/mL) was also assessed. Safety (adverse events [AEs] and serious AEs) was assessed after each dose. Overall, 336 participants were included (mean age: 60.2 years). Post-dose 3 seroprotection rates were: diphtheria, 94.6% (CI 91.5-96.8); tetanus and poliomyelitis, 100% (CI: 98.8-100). Percentage of participants with an antibody titre ≥ 5EU/mL against pertussis antigens was ≥ 95.8% for all five pertussis components. Basic clinical immunity against diphtheria was achieved in 100% (CI: 98.8-100) of participants. AEs were reported more frequently following vaccination with Tdap-IPV (post-dose 1: 65.3%) than with Td-IPV (post-dose 2: 48.3%; post-dose 3: 50.3%). This study highlights the benefits of using Tdap-IPV followed by two doses of Td-IPV in an

76 FR 27888 - Implantation or Injectable Dosage Form New Animal Drugs; Gonadotropin Releasing Factor-Diphtheria...

Science.gov (United States)

2011-05-13

... Factor-Diphtheria Toxoid Conjugate AGENCY: Food and Drug Administration, HHS. ACTION: Final rule. [[Page... veterinary prescription use of gonadotropin releasing factor-diphtheria toxoid conjugate by subcutaneous... provides for the veterinary prescription use of IMPROVEST (gonadotropin releasing factor-diphtheria toxoid...

Respiratory diphtheria in an asylum seeker from Afghanistan arriving to Finland via Sweden, December 2015.

Science.gov (United States)

Sane, Jussi; Sorvari, Tiina; Widerström, Micael; Kauma, Heikki; Kaukoniemi, Ulla; Tarkka, Eveliina; Puumalainen, Taneli; Kuusi, Markku; Salminen, Mika; Lyytikäinen, Outi

2016-01-01

In December 2015, an asylum seeker originating from Afghanistan was diagnosed with respiratory diphtheria in Finland. He arrived in Finland from Sweden where he had already been clinically suspected and tested for diphtheria. Corynebacterium diphtheriae was confirmed in Sweden and shown to be genotypically and phenotypically toxigenic. The event highlights the importance of early case detection, rapid communication within the country and internationally as well as preparedness plans of diphtheria antitoxin availability.

POTENSI GEN dtx DAN dtxR SEBAGAI MARKER UNTUK DETEKSI DAN PEMERIKSAAN TOKSIGENISITAS Corynebacterium diphtheriae

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Sunarno Sunarno

2013-05-01

Full Text Available Abstract.   Corynebacterium diphtheriae is the causative agent of diphtheria. The main virulence determinant of the bacteria is diphtheria toxin, the cause of the systemic complication seen with diphtheria. Production of diphtheria toxin by toxigenic strain encoded by dtx/tox gene and repressed by dtxR gene. Gold standard for bacterial toxigenicity test carried out by conventional methods (Elek test, Guinea pig and vero cell cytotoxicity. However, Elek test have variety result, time consume and problem of the reagent availability. On the other hand, the animal (Guinea pig testing was opposed by many animal lovers and the vero cell cytotoxicity test require high cost. The study purposed to evaluate the using of dtx and dtxR genes as a detection marker of C.diphtheriae and bacterial toxigenicity test simultaneusly by Multiplex PCR. The study examined 44 bacterial and fungal isolates, included 22 C.diphtheriae (4 reference strains and 18 clinical isolates, 5 other specieses of Corynebacterium  (reference strains and 17 non-Corynebacterium (10 reference strains and 7 stock cultures . All of sample were examined by Multiplex PCR for 2 primer pairs targeted dtx and dtxR genes. The study showed that the Multiplex PCR for dtx and dtxR as target genes able to detect all of sample correctly thus concluded that dtx and dtxR genes could be used as a marker for alternative detection and toxigenicity test of C.diphtheriae by Multiplex PCR rapidly and accuratelly. Key words: Corynebacterium diphtheriae, dtx, dan dtxR Abstrak. Corynebacterium diphtheriae merupakan agen penyebab penyakit difteri.. Faktor virulensi utama  C. diphtheriae adalah toksigenisitas (kemampuan memproduksi toksin bakteri toxin. Produksi toksin diatur seperangkat gen yang disebut gen tox/dtx dan diregulasi oleh gen dtxR. Gold standard untuk pemeriksaan toksigenisitas C.diphtheriae adalah dengan metode konvensional (Elek test, Guinea pig dan vero cell cytotoxigenicity,namun  Elek test

Influence of the shaft rotation on the stability of magnetic fluid shaft seal characteristics

Science.gov (United States)

Krakov, M. S.; Nikiforov, I. V.

2008-12-01

Distribution of the magnetic particles concentration in a magnetic fluid shaft seal is studied numerically for a rotating shaft. It is revealed that the shaft rotation causes not only an azimuthal flow of the magnetic fluid, but a meridional flow as well. This meridional flow prevents the growth of magnetic particle concentration in the gap of the magnetic fluid shaft seal. As a result, the burst pressure of the magnetic fluid shaft seal for the rotating shaft is stable and does not change with time. Figs 6, Refs 7.

DTaP Vaccine (Diphtheria, Tetanus, and Pertussis): What You Need to Know

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... STATEMENT DTaP Vaccine What You Need to Know (Diphtheria, Tetanus and Pertussis) Many Vaccine Information Statements are ... www. immunize. org/ vis 1 Why get vaccinated? Diphtheria, tetanus, and pertussis are serious diseases caused by ...

Pre-cementation of deep shaft

Science.gov (United States)

Heinz, W. F.

1988-12-01

Pre-cementation or pre-grouting of deep shafts in South Africa is an established technique to improve safety and reduce water ingress during shaft sinking. The recent completion of several pre-cementation projects for shafts deeper than 1000m has once again highlighted the effectiveness of pre-grouting of shafts utilizing deep slimline boreholes and incorporating wireline technique for drilling and conventional deep borehole grouting techniques for pre-cementation. Pre-cementation of deep shaft will: (i) Increase the safety of shaft sinking operation (ii) Minimize water and gas inflow during shaft sinking (iii) Minimize the time lost due to additional grouting operations during sinking of the shaft and hence minimize costly delays and standing time of shaft sinking crews and equipment. (iv) Provide detailed information of the geology of the proposed shaft site. Informations on anomalies, dykes, faults as well as reef (gold bearing conglomerates) intersections can be obtained from the evaluation of cores of the pre-cementation boreholes. (v) Provide improved rock strength for excavations in the immediate vicinity of the shaft area. The paper describes pre-cementation techniques recently applied successfully from surface and some conclusions drawn for further considerations.

Diphtheria, tetanus, and pertussis (DTaP) vaccines - what you need to know

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... is taken in its entirety from the CDC Diphtheria, Tetanus, and Pertussis (DTaP) Vaccine Information Statement (VIS): ... vis-statements/dtap.html CDC review information for Diphtheria, Tetanus, and Pertussis (DTaP) VIS: Page last reviewed: ...

The N-Terminal GYPSY Motif Is Required for Pilin-Specific Sortase SrtC1 Functionality in Lactobacillus rhamnosus Strain GG.

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François P Douillard

Full Text Available Predominantly identified in pathogenic Gram-positive bacteria, sortase-dependent pili are also found in commensal species, such as the probiotic-marketed strain Lactobacillus rhamnosus strain GG. Pili are typically associated with host colonization, immune signalling and biofilm formation. Comparative analysis of the N-terminal domains of pilin-specific sortases from various piliated Gram-positive bacteria identified a conserved motif, called GYPSY, within the signal sequence. We investigated the function and role of the GYPSY residues by directed mutagenesis in homologous (rod-shaped and heterologous (coccoid-shaped expression systems for pilus formation. Substitutions of some of the GYPSY residues, and more specifically the proline residue, were found to have a direct impact on the degree of piliation of Lb. rhamnosus GG. The present findings uncover a new signalling element involved in the functionality of pilin-specific sortases controlling the pilus biogenesis of Lb. rhamnosus GG and related piliated Gram-positive species.

The N-Terminal GYPSY Motif Is Required for Pilin-Specific Sortase SrtC1 Functionality in Lactobacillus rhamnosus Strain GG

Science.gov (United States)

Douillard, François P.; Rasinkangas, Pia; Bhattacharjee, Arnab; Palva, Airi; de Vos, Willem M.

2016-01-01

Predominantly identified in pathogenic Gram-positive bacteria, sortase-dependent pili are also found in commensal species, such as the probiotic-marketed strain Lactobacillus rhamnosus strain GG. Pili are typically associated with host colonization, immune signalling and biofilm formation. Comparative analysis of the N-terminal domains of pilin-specific sortases from various piliated Gram-positive bacteria identified a conserved motif, called GYPSY, within the signal sequence. We investigated the function and role of the GYPSY residues by directed mutagenesis in homologous (rod-shaped) and heterologous (coccoid-shaped) expression systems for pilus formation. Substitutions of some of the GYPSY residues, and more specifically the proline residue, were found to have a direct impact on the degree of piliation of Lb. rhamnosus GG. The present findings uncover a new signalling element involved in the functionality of pilin-specific sortases controlling the pilus biogenesis of Lb. rhamnosus GG and related piliated Gram-positive species. PMID:27070897

External quality assessments for microbiologic diagnosis of diphtheria in Europe.

Science.gov (United States)

Both, Leonard; Neal, Shona; De Zoysa, Aruni; Mann, Ginder; Czumbel, Ida; Efstratiou, Androulla

2014-12-01

The European Diphtheria Surveillance Network (EDSN) ensures the reliable epidemiological and microbiologic assessment of disease prevalence in the European Union. Here, we describe a survey of current diagnostic techniques for diphtheria surveillance conducted across the European Union and report the results from three external quality assessment (EQA) schemes performed between 2010 and 2014. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Rotary shaft seal

International Nuclear Information System (INIS)

Langebrake, C.O.

1984-01-01

The invention is a novel rotary shaft seal assembly which provides positive-contact sealing when the shaft is not rotated and which operates with its sealing surfaces separated by a film of compressed ambient gas whose width is independent of the speed of shaft rotation. In a preferred embodiment, the assembly includes a disc affixed to the shaft for rotation therewith. Axially movable, non-rotatable plates respectively supported by sealing bellows are positioned on either side of the disc to be in sealing engagement therewith. Each plate carries piezoelectric transducer elements which are electrically energized at startup to produce films of compressed ambient gas between the confronting surfaces of the plates and the disc. Following shutdown of the shaft, the transducer elements are de-energized. A control circuit responds to incipient rubbing between the plate and either disc by altering the electrical input to the transducer elements to eliminate rubbing

Does vaccination ensure protection? Assessing diphtheria and tetanus antibody levels in a population of healthy children

Science.gov (United States)

Gowin, Ewelina; Wysocki, Jacek; Kałużna, Ewelina; Świątek-Kościelna, Bogna; Wysocka-Leszczyńska, Joanna; Michalak, Michał; Januszkiewicz-Lewandowska, Danuta

2016-01-01

Abstract Vaccination effectiveness is proven when the disease does not develop after a patient is exposed to the pathogen. In the case of rare diseases, vaccination effectiveness is assessed by monitoring specific antibody levels in the population. Such recurrent analyses allow the evaluation of vaccination programs. The primary schedule of diphtheria and tetanus vaccinations is similar in various countries, with differences mainly in the number and timing of booster doses. The aim of the study was to assess diphtheria and tetanus antibody concentrations in a population of healthy children. Diphtheria and tetanus antibody levels were analyzed in a group of 324 children aged 18 to 180 months. All children were vaccinated in accordance with the Polish vaccination schedule. Specific antibody concentrations greater than 0.1 IU/mL were considered protective against tetanus or diphtheria. Levels above 1.0 were considered to ensure long-term protection. Protective levels of diphtheria antibodies were found in 229 patients (70.46%), and of tetanus in 306 patients (94.15%). Statistically significant differences were found in tetanus antibody levels in different age groups. Mean concentrations and the percentage of children with high tetanus antibody titers increased with age. No similar correlation was found for diphtheria antibodies. High diphtheria antibody levels co-occurred in 72% of the children with high tetanus antibody levels; 95% of the children with low tetanus antibody levels had low levels of diphtheria antibodies. The percentage of children with protective diphtheria antibody levels is lower than that in the case of tetanus antibodies, both in Poland and abroad, but the high proportion of children without diphtheria protection in Poland is an exception. This is all the more puzzling when taking into account that Polish children are administered a total of 5 doses containing a high concentration of diphtheria toxoid, at intervals shorter than 5 years. The

Anti-idiotypic antibodies that protect cells against the action of diphtheria toxin

International Nuclear Information System (INIS)

Rolf, J.M.; Gaudin, H.M.; Tirrell, S.M.; MacDonald, A.B.; Eidels, L.

1989-01-01

An anti-idiotypic serum prepared against the combining site (idiotype) of specific anti-diphtheria toxoid antibodies was characterized with respect to its interaction with highly diphtheria toxin-sensitive Vero cells. Although the anti-idiotypic serum protected Vero cells against the cytotoxic action of diphtheria toxin, it did not prevent the binding of 125 I-labeled diphtheria toxin to the cells but did inhibit the internalization and degradation of 125 I-labeled toxin. This anti-idiotypic serum immunoprecipitated a cell-surface protein from radiolabeled Vero cells with an apparent Mr of approximately 15,000. These results are consistent with the hypothesis that the anti-idiotypic serum contains antibodies that carry an internal image of an internalization site on the toxin and that a cell-surface protein involved in toxin internalization possesses a complementary site recognized by both the toxin and the anti-idiotypic antibodies

Diphtheria toxin translocation across cellular membranes is regulated by sphingolipids

International Nuclear Information System (INIS)

Spilsberg, Bjorn; Hanada, Kentaro; Sandvig, Kirsten

2005-01-01

Diphtheria toxin is translocated across cellular membranes when receptor-bound toxin is exposed to low pH. To study the role of sphingolipids for toxin translocation, both a mutant cell line lacking the first enzyme in de novo sphingolipid synthesis, serine palmitoyltransferase, and a specific inhibitor of the same enzyme, myriocin, were used. The serine palmitoyltransferase-deficient cell line (LY-B) was found to be 10-15 times more sensitive to diphtheria toxin than the genetically complemented cell line (LY-B/cLCB1) and the wild-type cell line (CHO-K1), both when toxin translocation directly across the plasma membrane was induced by exposing cells with surface-bound toxin to low pH, and when the toxin followed its normal route via acidified endosomes into the cytosol. Toxin binding was similar in these three cell lines. Furthermore, inhibition of serine palmitoyltransferase activity by addition of myriocin sensitized the two control cell lines (LY-B/cLCB1 and CHO-K1) to diphtheria toxin, whereas, as expected, no effect was observed in cells lacking serine palmitoyltransferase (LY-B). In conclusion, diphtheria toxin translocation is facilitated by depletion of membrane sphingolipids

[Diphtheria in the military forces: lessons and current status of prophylaxis, prospects of epidemiological control process].

Science.gov (United States)

Belov, A B; Ogarkov, P I

2014-01-01

We analyzed the epidemiological situation of diphtheria in the world and in Russia and experience of mass vaccination of military personnel and civil population with diphtheria toxoid for the last 50 years. Early diagnosis of diphtheria in military personnel has a prognostic value. Authors described the peculiarities of epidemiological process of diphtheria in military personnel in 80-90 years of 20th century and organizational aspects of mass vaccination with diphtheria toxoid. Authors analyzed current problems of epidemiology and prophylaxis of diphtheria in military personnel and civil population and possible developments. According to long-term prognosis authors mentioned the increase of morbidity and came to conclusion that it is necessary enhance the epidemiological surveillance. Authors presented prospect ways of improvement of vaccination and rational approaches to immunization of military personnel under positive long-term epidemiological situation.

Resurgence of diphtheria in rural areas of North Karnataka, India

Directory of Open Access Journals (Sweden)

Mahantesh V Parande

2017-01-01

Full Text Available Background: A diphtheria outbreak was identified from Vijayapura (formerly Bijapur district in the South Indian state of Karnataka in 2011. There was a surge in the number of throat swab samples received under the Integrated Disease Surveillance Programme (IDSP in North Karnataka since then. Objectives: A microbiological study was undertaken to generate information on the status of resurgence of the disease in the region. Materials and Methods: Throat swabs from 432 suspected cases of diphtheria during 2012–2015 were obtained from government hospitals and primary health centres of 8 districts in North Karnataka and were processed for the culture and identification of Corynebacterium diphtheriae. Polymerase chain reaction for the presence of toxin gene (toxA and toxB was carried out on the isolates. Antibiotic sensitivity tests were performed on the isolates with a panel of 14 antibiotics. Results: Thirty-eight (8.79% out of 432 samples yielded C. diphtheriae on culture. All isolates possessed the diphtheria toxin gene. Out of the 38 confirmed cases, whereas 21 (55.26% were between 1 and 5 years of age, 14 (36.84% were aged between 5 and 10 years. Male children were three times more than females in confirmed cases. No information was available on the immunisation status of the cases. Emergence of resistance to penicillin was found with minimum inhibitory concentration reaching up to 6.00 μg/ml. Conclusion and Discussion: Our study identified an upsurge in cases of diphtheria in North Karnataka, particularly in Vijayapura District, and to the best of our knowledge, reports the emergence of penicillin resistance for the first time in India. The study calls for enhanced surveillance for the disease, making antidiphtheritic serum available in key hospitals in the region and serves to provide a baseline for future assessment of the impact of the recently launched 'Mission Indradhanush' programme in strengthening Universal Immunisation Programme

Anti-idiotypic antibodies that protect cells against the action of diphtheria toxin

Energy Technology Data Exchange (ETDEWEB)

Rolf, J.M.; Gaudin, H.M.; Tirrell, S.M.; MacDonald, A.B.; Eidels, L.

1989-03-01

An anti-idiotypic serum prepared against the combining site (idiotype) of specific anti-diphtheria toxoid antibodies was characterized with respect to its interaction with highly diphtheria toxin-sensitive Vero cells. Although the anti-idiotypic serum protected Vero cells against the cytotoxic action of diphtheria toxin, it did not prevent the binding of /sup 125/I-labeled diphtheria toxin to the cells but did inhibit the internalization and degradation of /sup 125/I-labeled toxin. This anti-idiotypic serum immunoprecipitated a cell-surface protein from radiolabeled Vero cells with an apparent Mr of approximately 15,000. These results are consistent with the hypothesis that the anti-idiotypic serum contains antibodies that carry an internal image of an internalization site on the toxin and that a cell-surface protein involved in toxin internalization possesses a complementary site recognized by both the toxin and the anti-idiotypic antibodies.

Seroepidemiology of diphtheria and tetanus among children and young adults in Tajikistan: nationwide population-based survey, 2010.

Science.gov (United States)

Khetsuriani, Nino; Zakikhany, Katherina; Jabirov, Shamsiddin; Saparova, Nargis; Ursu, Pavel; Wannemuehler, Kathleen; Wassilak, Steve; Efstratiou, Androulla; Martin, Rebecca

2013-10-01

Tajikistan had a major diphtheria outbreak (≈ 10,000 cases) in the 1990 s, which was controlled after nationwide immunization campaigns with diphtheria-tetanus toxoid in 1995 and 1996. Since 2000, only 52 diphtheria cases have been reported. However, in coverage surveys conducted in 2000 and 2005, diphtheria-tetanus-pertussis vaccine coverage was lower than administratively reported estimates raising concerns about potential immunity gaps. To further assess population immunity to diphtheria in Tajikistan, diphtheria antibody testing was included in a large-scale nationwide serosurvey for vaccine-preventable diseases conducted in connection with a poliomyelitis outbreak in 2010. In addition, the serosurvey provided an opportunity to assess population immunity to tetanus. Residents of all regions of Tajikistan aged 1-24 years were included in the serosurvey implemented during September-October 2010. Participants were selected through stratified cluster sampling. Specimens were tested for diphtheria antibodies using a Vero cell neutralization assay and for tetanus antibodies using an anti-tetanus IgG ELISA. Antibody concentrations ≥ 0.1 IU/mL were considered seropositive. Overall, 51.4% (95% CI, 47.1%-55.6%) of participants were seropositive for diphtheria and 78.9% (95% CI, 74.7%-82.5%) were seropositive for tetanus. The lowest percentages of seropositivity for both diseases were observed among persons aged 10-19 years: diphtheria seropositivity was 37.1% (95% CI, 31.0%-43.7%) among 10-14 year-olds, and 35.3% (95% CI, 29.9%-41.1%) among 15-19 year-olds; tetanus seropositivity in respective age groups was 65.3% (95% CI, 58.4%-71.6%) and 70.1% (95% CI, 64.5%-75.2%). Population immunity for diphtheria in Tajikistan is low, particularly among 10-19 year-olds. Population immunity to tetanus is generally higher than for diphtheria, but is suboptimal among 10-19 year-olds. These findings highlight the need to improve routine immunization service delivery, and support a

Tetanus and diphtheria immunity in refugees in Europe in 2015.

Science.gov (United States)

Jablonka, Alexandra; Behrens, Georg M N; Stange, Marcus; Dopfer, Christian; Grote, Ulrike; Hansen, Gesine; Schmidt, Reinhold Ernst; Happle, Christine

2017-04-01

Current political crises in the Middle East and economic discrepancies led millions of people to leave their home countries and to flee to Western Europe. This development raises unexpected challenges for receiving health care systems. Although pan-European initiatives strive for updated and optimized vaccination strategies, little data on immunity against vaccine-preventable diseases in the current refugee population exist. We quantified serum IgG against tetanus and diphtheria (TD) in n = 678 refugees currently seeking shelter in six German refugee centers. Reflecting current migration statistics in Europe, the median age within the cohort was 26 years, with only 23.9 % of female subjects. Insufficient IgG levels without long-term protection against tetanus were found in 56.3 % of all refugees. 76.1 % of refugees had no long-term protection against diphtheria. 47.7 % of subjects needed immediate vaccination against tetanus, and 47.7 % against diphtheria. For both diseases, an age-dependent decline in protective immunity occurred. We observed a considerably low rate of tetanus-protected refugees, and the frequency of diphtheria-immune refugees was far from sufficient to provide herd immunity. These findings strongly support recent intentions to implement and enforce stringent guidelines for refugee vaccination in the current crisis.

Access to diphtheria antitoxin for therapy and diagnostics.

Science.gov (United States)

Both, L; White, J; Mandal, S; Efstratiou, A

2014-06-19

The most effective treatment for diphtheria is swift administration of diphtheria antitoxin (DAT) with conjunct antibiotic therapy. DAT is an equine immunoglobulin preparation and listed among the World Health Organization Essential Medicines. Essential Medicines should be available in functioning health systems at all times in adequate amounts, in appropriate dosage forms, with assured quality, and at prices individuals and the community can afford. However, DAT is in scarce supply and frequently unavailable to patients because of discontinued production in several countries, low economic viability, and high regulatory requirements for the safe manufacture of blood-derived products. DAT is also a cornerstone of diphtheria diagnostics but several diagnostic reference laboratories across the European Union (EU) and elsewhere routinely face problems in sourcing DAT for toxigenicity testing. Overall, global access to DAT for both therapeutic and diagnostic applications seems inadequate. Therefore--besides efforts to improve the current supply of DAT--accelerated research and development of alternatives including monoclonal antibodies for therapy and molecular-based methods for diagnostics are required. Given the rarity of the disease, it would be useful to organise a small stockpile centrally for all EU countries and to maintain an inventory of DAT availability within and between countries.

Molecular and Epidemiological Review of Toxigenic Diphtheria Infections in England between 2007 and 2013

Science.gov (United States)

Both, Leonard; Collins, Sarah; de Zoysa, Aruni; White, Joanne; Mandal, Sema

2014-01-01

Human infections caused by toxigenic corynebacteria occur sporadically across Europe. In this report, we undertook the epidemiological and molecular characterization of all toxigenic corynebacterium strains isolated in England between January 2007 and December 2013. Epidemiological aspects include case demographics, risk factors, clinical presentation, treatment, and outcome. Molecular characterization was performed using multilocus sequence typing (MLST) alongside traditional phenotypic methods. In total, there were 20 cases of toxigenic corynebacteria; 12 (60.0%) were caused by Corynebacterium ulcerans, where animal contact was the predominant risk factor. The remaining eight (40.0%) were caused by Corynebacterium diphtheriae strains; six were biovar mitis, which were associated with recent travel abroad. Adults 45 years and older were particularly affected (55.0%; 11/20), and typical symptoms included sore throat and fever. Respiratory diphtheria with the absence of a pharyngeal membrane was the most common presentation (50.0%; 10/20). None of the eight C. diphtheriae cases were fully immunized. Diphtheria antitoxin was issued in two (9.5%) cases; both survived. Two (9.5%) cases died, one due to a C. diphtheriae infection and one due to C. ulcerans. MLST demonstrated that the majority (87.5%; 7/8) of C. diphtheriae strains represented new sequence types (STs). By adapting several primer sequences, the MLST genes in C. ulcerans were also amplified, thereby providing the basis for extension of the MLST scheme, which is currently restricted to C. diphtheriae. Despite high population immunity, occasional toxigenic corynebacterium strains are identified in England and continued surveillance is required. PMID:25502525

Genetic relationships of Corynebacterium diphtheriae strains isolated from a diphtheria case and carriers by restriction fragment length polymorphism of rRNA genes Relação genética de cepas de Corynebacterium diphtheriae isoladas de caso e seus contatos por RLFP de rRNA gene

Directory of Open Access Journals (Sweden)

Claudio Tavares Sacchi

1995-08-01

Full Text Available In the present study we report the results of an analysis, based on ribotyping of Corynebacterium diphtheriae intermedius strains isolated from a 9 years old child with clinical diphtheria and his 5 contacts. Quantitative analysis of RFLPs of rRNA was used to determine relatedness of these 7 C.diphtheriae strains providing support data in the diphtheria epidemiology. We have also tested those strains for toxigenicity in vitro by using the Elek's gel diffusion method and in vivo by using cell culture method on cultured monkey kidney cell (VERO cells. The hybridization results revealed that the 5 C.diphtheriae strains isolated from contacts and one isolated from the clinical case (nose case strain had identical RFLP patterns with all 4 restriction endonucleases used, ribotype B. The genetic distance from this ribotype and ribotype A (throat case strain, that we initially assumed to be responsible for the illness of the patient, was of 0.450 showing poor genetic correlation among these two ribotypes. We found no significant differences concerned to the toxin production by using the cell culture method. In conclusion, the use of RFLPs of rRNA gene was successful in detecting minor differences in closely related toxigenic C.diphtheriae intermedius strains and providing information about genetic relationships among them.No presente estudo, nós reportamos os resultados de uma análise, baseada na ribotipagem de cepas de C. diphtheriae intermedius isoladas de uma criança de 9 anos com difteria e seus 5 contatos. Análise quantitativa por RFLP de rRNA foi usada para determinar a relação destas 7 cepas de C. diphtheriae fornecendo dados de interesse epidemiológico. Nós também testamos estas cepas para toxicidade in vitro usando método de difusão de Elek e in vivo usando método de cultura celular com células VERO. Os resultados de hibridização revelaram que as 5 cepas de C. diphtheriae isoladas dos contatos e uma isolada do caso (cepa isolada

A case of pharyngeal diphtheria in Germany, June 2015.

Science.gov (United States)

Berger, A; Meinel, D M; Schaffer, A; Ziegler, R; Pitteroff, J; Konrad, R; Sing, Andreas

2016-10-01

In June 2015, a 45-year-old man suffering from acute necrotic tonsillitis and throat phlegmon was hospitalized in Nuremberg, Germany. After emergency surgery the patient was initially treated with antibiotics. A throat swab grew a toxigenic Corynebacterium diphtheriae biovar mitis strain. The patient's vaccination status was not documented and the patient was tested serologically for anti-diphtheria antibodies showing no protective immunity. Extensive control investigations were performed by the local health department showing no likely source of his infection. No secondary cases were found and the patient completely recovered.

ORIGINAL ARTICLE DIPHTHERIA MORTALITY IN NIGERIA: THE ...

African Journals Online (AJOL)

Dr Oboro VO

AFRICAN JOURNAL OF CLINICAL AND EXPERIMENTAL MICROBIOLOGY MAY 2011 ... RESULTS: Nine cases of diphtheria were seen and three mortalities were recorded giving a mortality rate ... tissue edema and airway obstruction by the.

Potency of a human monoclonal antibody to diphtheria toxin relative to equine diphtheria anti-toxin in a guinea pig intoxication model.

Science.gov (United States)

Smith, Heidi L; Cheslock, Peter; Leney, Mark; Barton, Bruce; Molrine, Deborah C

2016-08-17

Prompt administration of anti-toxin reduces mortality following Corynebacterium diphtheriae infection. Current treatment relies upon equine diphtheria anti-toxin (DAT), with a 10% risk of serum sickness and rarely anaphylaxis. The global DAT supply is extremely limited; most manufacturers have ceased production. S315 is a neutralizing human IgG1 monoclonal antibody to diphtheria toxin that may provide a safe and effective alternative to equine DAT and address critical supply issues. To guide dose selection for IND-enabling pharmacology and toxicology studies, we dose-ranged S315 and DAT in a guinea pig model of diphtheria intoxication based on the NIH Minimum Requirements potency assay. Animals received a single injection of antibody premixed with toxin, were monitored for 30 days, and assigned a numeric score for clinical signs of disease. Animals receiving ≥ 27.5 µg of S315 or ≥ 1.75 IU of DAT survived whereas animals receiving ≤ 22.5 µg of S315 or ≤ 1.25 IU of DAT died, yielding a potency estimate of 17 µg S315/IU DAT (95% CI 16-21) for an endpoint of survival. Because some surviving animals exhibited transient limb weakness, likely a systemic sign of toxicity, DAT and S315 doses required to prevent hind limb paralysis were also determined, yielding a relative potency of 48 µg/IU (95% CI 38-59) for this alternate endpoint. To support advancement of S315 into clinical trials, potency estimates will be used to evaluate the efficacy of S315 versus DAT in an animal model with antibody administration after toxin exposure, more closely modeling anti-toxin therapy in humans.

Extension of the Consolidation 3 shaft

Energy Technology Data Exchange (ETDEWEB)

Bohnenkamp, G [Gesteins- und Tiefbau G.m.b.H., Recklinghausen (Germany, F.R.)

1978-02-01

The conversion of a mine shaft into a central winning shaft is described, in particular planning principles, problems to be solved, preliminary work, timber drawing, extension work, shaft deepening, and the installation of shaft internals.

Designing vertical mine shafts under conditions of increasing shaft depth with rock hoisting to the operating mining level

Energy Technology Data Exchange (ETDEWEB)

Durov, E.M.

1983-05-01

A system for shaft excavation in deep coal mines with mining depth exceeding 1,000 m is discussed. During mine sinking rocks are removed to the ground surface. When depth of a deep mine shaft is increased rocks are removed to the operating mining level, causing lower investment costs than the system with rock hoisting to the ground surface. The Yuzhgiproshakht design firm carries out investigations on the optimum methods for increasing shaft depth in coal mines. Coal mines with the following coal output are included in evaluations: 0.9, 1.2, 1.5, and 1.8 Mt/year. Mine shaft depth of 600, 800, 1000, 1200, 1400 and 1600 m is analyzed. Shaft depth is increased by 100, 200, 300 or 400 m. Shaft sinking rate ranges from 10 to 70 m/month. Effects of rock hoisting from the shaft bottom on the hoisting scheme in a mine shaft are analyzed. Position of hoisting bucket in relation to cages or skips moving in a shaft is investigated. Investigation results are given in 5 schemes. Analyses show that use of a shaft sinking system with rock hoisting to the ground surface during shaft excavation and with rock hoisting to the operating mining level during shaft depth increasing is economical when a shaft with skips is from 7 to 8 m in diameter or when a cage shaft is 6 m, 7 m or 8 m in diameter. Use of standardized shaft excavation systems is recommended. (In Russian)

Binding of Diphtheria Toxin to Phospholipids in Liposomes

Science.gov (United States)

Alving, Carl R.; Iglewski, Barbara H.; Urban, Katharine A.; Moss, Joel; Richards, Roberta L.; Sadoff, Jerald C.

1980-04-01

Diphtheria toxin bound to the phosphate portion of some, but not all, phospholipids in liposomes. Liposomes consisting of dimyristoyl phosphatidylcholine and cholesterol did not bind toxin. Addition of 20 mol% (compared to dimyristoyl phosphatidylcholine) of dipalmitoyl phosphatidic acid, dicetyl phosphate, phosphatidylinositol phosphate, cardiolipin, or phosphatidylserine in the liposomes resulted in substantial binding of toxin. Inclusion of phosphatidylinositol in dimyristol phosphatidylcholine / cholesterol liposomes did not result in toxin binding. The calcium salt of dipalmitoyl phosphatidic acid was more effective than the sodium salt, and the highest level of binding occurred with liposomes consisting only of dipalmitoyl phosphatidic acid (calcium salt) and cholesterol. Binding of toxin to liposomes was dependent on pH, and the pattern of pH dependence varied with liposomes having different compositions. Incubation of diphtheria toxin with liposomes containing dicetyl phosphate resulted in maximal binding at pH 3.6, whereas binding to liposomes containing phosphatidylinositol phosphate was maximal above pH 7. Toxin did not bind to liposomes containing 20 mol% of a free fatty acid (palmitic acid) or a sulfated lipid (3-sulfogalactosylceramide). Toxin binding to dicetyl phosphate or phosphatidylinositol phosphate was inhibited by UTP, ATP, phosphocholine, or p-nitrophenyl phosphate, but not by uracil. We conclude that (a) diphtheria toxin binds specifically to the phosphate portion of certain phospholipids, (b) binding to phospholipids in liposomes is dependent on pH, but is not due only to electrostatic interaction, and (c) binding may be strongly influenced by the composition of adjacent phospholipids that do not bind toxin. We propose that a minor membrane phospholipid (such as phosphatidylinositol phosphate or phosphatidic acid), or that some other phosphorylated membrane molecule (such as a phosphoprotein) may be important in the initial binding of

Circumferential shaft seal

Science.gov (United States)

Ludwig, L. P. (Inventor)

1981-01-01

A circumferential shaft seal comprising two sealing rings held to a rotating shaft by means of a surrounding elastomeric band is disclosed. The rings are segmented and are of a rigid sealing material such as carbon or a polyimide and graphite fiber composite.

Type IV pilins regulate their own expression via direct intramembrane interactions with the sensor kinase PilS

OpenAIRE

Kilmury, Sara L. N.; Burrows, Lori L.

2016-01-01

Although two-component systems are a ubiquitous means of rapid bacterial adaptation to changing environments, identification of the specific signals detected by sensor kinases can be challenging. Also, little is known about the diverse, poorly characterized family of sensor kinases that detect intramembrane signals. We show that the major type IV pilin, PilA, is an inhibitory intramembrane ligand for the PilS sensor kinase that controls pilA expression and we characterize the mechanism of sig...

Technetium-99m labeling and fibronectin binding ability of Corynebacterium diphtheriae

International Nuclear Information System (INIS)

Souza, S.M.S.; Nagao, P.E.; Bernardo-Filho, M.; Pereira, G.A.; Napoleao, F.; Andrade, A.F.B.; Hirata Junior, R.; Mattos-Guaraldi, A.L.

2004-01-01

The use of radionuclides has permitted advances in areas of clinical and scientific knowledge. Several molecules and cells have been labelled with Technetium-99m ( 99m Tc). The stannous chloride (SnCl 2 ) has a significant influence on the labeling and stability of 99m Tc radiotracers. The frequent risk of diphtheria epidemics has intensified interest in the virulence factors of Corynebacterium diphtheriae. Although studies have looked at potential adhesins including haemagglutinins and exposed sugar residues, the molecular basis of mechanisms of adherence remains unclear. Adherence of pathogens to mammalian tissues may be mediated by fibronectin (FN) found in body fluids, matrix of connective tissues, and cell surfaces. In the present study we evaluated the binding ability to human plasma FN by 99m Tc labeled-C.diphtheriae. Due to adverse effects of stannous ions, microorganisms were submitted to survival and filamentation induction assays. Data showed a dose dependent susceptibility to SnCl 2 bactericidal effects. Cell filamentation was observed for concentrations of SnCl 2 > 110 μg/ml. Adherence levels of 99m Tc labelled 241strain to coverslips coated with 20 μg/ml FN were higher (P = 0.0037) than coated with bovine serum albumin. FN binding by the sucrose fermenting 241 C. diphtheriae strain (8.9% + 2.6) was significantly lower (P=0.0139) than Staphylococcus aureus Cowan I strain (34.1% ± 1.2). Therefore, bacterial 99m Tc labeling represents an additional tool that may contribute to the comprehension of C. diphtheriae interactions with host receptors such as FN that act as biological organizers by holding bacterial cells in position and guiding their migration. (author)

Durability of Vaccine-Induced Immunity Against Tetanus and Diphtheria Toxins: A Cross-sectional Analysis

Science.gov (United States)

Hammarlund, Erika; Thomas, Archana; Poore, Elizabeth A.; Amanna, Ian J.; Rynko, Abby E.; Mori, Motomi; Chen, Zunqiu; Slifka, Mark K.

2016-01-01

Background. Many adult immunization schedules recommend that tetanus and diphtheria vaccination be performed every 10 years. In light of current epidemiological trends of disease incidence and rates of vaccine-associated adverse events, the 10-year revaccination schedule has come into question. Methods. We performed cross-sectional analysis of serum antibody titers in 546 adult subjects stratified by age or sex. All serological results were converted to international units after calibration with international serum standards. Results. Approximately 97% of the population was seropositive to tetanus and diphtheria as defined by a protective serum antibody titer of ≥0.01 IU/mL. Mean antibody titers were 3.6 and 0.35 IU/mL against tetanus and diphtheria, respectively. Antibody responses to tetanus declined with an estimated half-life of 14 years (95% confidence interval, 11–17 years), whereas antibody responses to diphtheria were more long-lived and declined with an estimated half-life of 27 years (18–51 years). Mathematical models combining antibody magnitude and duration predict that 95% of the population will remain protected against tetanus and diphtheria for ≥30 years without requiring further booster vaccination. Conclusions. These studies demonstrate that durable levels of protective antitoxin immunity exist in the majority of vaccinated individuals. Together, this suggests that it may no longer be necessary to administer booster vaccinations every 10 years and that the current adult vaccination schedule for tetanus and diphtheria should be revisited. PMID:27060790

Immunity to Diphtheria and Tetanus in Army Personnel and Adult Civilians in Mashhad, Iran.

Science.gov (United States)

Hosseini Shokouh, Seyyed Javad; Mohammadi, Babak; Rajabi, Jalil; Mohammadian Roshan, Ghasem

2017-03-24

This study aimed to investigate serologic immunity to diphtheria and tetanus in army personnel and a sample population of adult civilians in Mashhad, Iran. Army personnel (n = 180) and civilians (n = 83) who presented at Mashhad army hospital participated in this study. Diphtheria and tetanus antitoxin levels were determined by enzyme-linked immunosorbent assay. Approximately 77% and 94% of army personnel aged 18-34 years had at least basic protection against diphtheria (antitoxin level ≥0.1 IU/mL) and tetanus (antitoxin level >0.1 IU/mL), respectively. For civilians in this age group, the proportions were 76% for both diseases. Antitoxin levels waned with age. Thus, participants older than 50 years had lower immunity; this decrease in immunity was more pronounced for tetanus than for diphtheria in both army personnel and civilians. For both diseases, geometric mean antitoxin titers and the proportion of participants with at least basic protection were higher in subjects with a history of vaccination in the last 10 years (P diphtheria and tetanus. However, the large number of susceptible older adults (>50 years old) calls for improved booster vaccination protocols.

Seroepidemiology of diphtheria and pertussis in Beijing, China: A cross-sectional study.

Science.gov (United States)

Li, Xiaomei; Chen, Meng; Zhang, Tiegang; Li, Juan; Zeng, Yang; Lu, Li

2015-01-01

The aim of this study was to assess the level of humoral immunity against diphtheria and pertussis by measuring IgG to diphtheria toxoid (DT) and pertussis toxin (PT) in general population of Beijing. A total of 2147 subjects aged 0-74 y were selected with a random sample of resident population in Beijing. The information of socio-demographic characteristics, vaccination history, disease history of diphtheria and pertussis were collected for each subject by questionnaire. Serum samples were tested for IgG antibodies to DT and PT by using commercial ELISA kits. The overall positivity rate of anti-DT IgG was 66.28% with the mean concentration of 2.169 IU/ml. Age stratified data showed that the highest positivity rate of 97.63% was observed in 1-4 y and the rates decreased with age. The positivity rates were only around 50% or below since 25 y old. The positivity rate of anti-PT IgG was 12.34% with the mean concentration of 15.163 IU/ml. The highest level of positivity rate (22.23%) and antibody level (23.101 IU/ml) was seen in diphtheria was observed at 1 y and 6 y respectively, which was consistent with the current immunization schedule. But there was no significant increase of immunity to pertussis observed after booster immunization at 18-24 months, but the proportions of undetectable were lowest in diphtheria and all the age groups showed a low immunity to pertussis indicating the potential risk of transmission and outbreaks of the 2 diseases in Beijing.

An exploratory shaft facility in SALT: Draft shaft study plan

International Nuclear Information System (INIS)

1987-03-01

This draft Shaft Study Plan describes a program of testing and monitoring in the Exploratory Shafts of a candidate high-level nuclear waste repository site in Deaf Smith County, Texas. The purpose of the programs to assist with site characterization in support of a determination of site suitability for development as a repository design and performance assessment evaluations. The program includes a variety of geological, geophysical, geomechanical, thermomechanical, and geohydrological testing and monitoring. The program is presented as a series of separate studies concerned with geological, geomechanical, and geohydrological site characterization, and with evaluating the mechanical and hydrological response of the site to construction of the shafts. The various studies, and associated test or monitoring methods are shown. The procedure used in developing the test program has been to initially identify the information necessary to satisfy (1) federal, state, and local requirements, and (2) repository program requirements. These information requirements have then been assessed to determine which requirements can be addressed wholly or in significant part by monitoring and testing from within the shafts. Test methods have been identified to address specific information requirements. 67 refs., 39 figs., 31 tabs

Shaft siting decision

International Nuclear Information System (INIS)

1987-08-01

This study identifies and establishes relative guidelines to be used for siting of repository shafts. Weights were determined for the significant factors that impact the selection of shaft locations for a nuclear waste repository in salt. The study identified a total of 45 factors. A panel of experienced mining people utilized the Kepner-Tregoe (K-T) Decision Analysis Process to perform a structured evaluation of each significant shaft siting factor. The evaluation determined that 22 of the factors were absolute constraints and that the other 23 factors were desirable characteristics. The group established the relative weights for each of the 23 desirable characteristics by using a paired comparison method. 8 refs., 2 figs., 5 tabs

Seroprevalence of antibody against diphtheria among the population in Khon Kaen province, Thailand.

Science.gov (United States)

Bansiddhi, Hataichanok; Vuthitanachot, Viboonsuk; Vuthitanachot, Chanpim; Prachayangprecha, Slinporn; Theamboonlers, Apiradee; Poovorawan, Yong

2015-03-01

To assess diphtheria immunity in the northeastern region of Thailand, a seroepidemiological survey was undertaken in 2011 from 516 healthy individuals (age range 2-87 years) in Khon Kaen province. Diphtheria antitoxin levels were measured by enzyme-linked immunosorbent assay and titers of ≥0.1 IU/mL were considered to be protective antitoxin levels. Among the studied population, 94.8% have fully protective levels. The younger population (age range 2-19 years) has higher diphtheria immunity with seroprotection rates of 96.8% to 97.9%, compared with the adult population. The proportion of protective diphtheria antitoxin levels declines to 88.3% to 91.9% in the middle-aged group (20-50 years), and appeared to be higher again in the older age-group (50-70 years). To avoid epidemic spreading, promoting immunization booster programs will be helpful, especially among the adult population (20-50 years). Finally, this study may serve as a valuable guide in deciding exactly which age-groups should be targeted by such an effort. © 2012 APJPH.

Enacting cultural boundaries in French and German diphtheria serum research.

Science.gov (United States)

Klöppel, Ulrike

2008-06-01

The experimental development of a therapeutic serum against diphtheria between 1891 and 1894 was characterized by a scientific competition that pitted Emil Behring from the Institute for Infectious Diseases in Berlin against Emile Roux and Elie Metschnikoff from the Pasteur Institute in Paris. In general, their competition can be regarded as an extension of the fundamental differences that separated the research schools of Robert Koch and Louis Pasteur. However, to characterize the competition for a diphtheria-serum as "national rivalry" fails to account adequately for the mutual adoption of experimental practices by the Berlin and Parisian protagonists, whose contributions to the development of a therapeutic serum were intertwined in complex ways. Nor can it be characterized as "cooperation," given their fierce public disputes over scientific concepts and the fact that these disputes also shaped the peculiarities of the experimental procedures in Berlin and Paris. A close analysis reveals a complex picture of the dynamic interaction between the conceptual and experimental activities of Behring, Roux, and Metschnikoff- interaction that defined as well as bridged the "French" and "Prussian" experimental systems of diphtheria-serum research.

Recall Responses to Tetanus and Diphtheria Vaccination Are Frequently Insufficient in Elderly Persons

Science.gov (United States)

Weinberger, Birgit; Schirmer, Michael; Matteucci Gothe, Raffaella; Siebert, Uwe; Fuchs, Dietmar; Grubeck-Loebenstein, Beatrix

2013-01-01

Demographic changes and a more active life-style in older age have contributed to an increasing public awareness of the need for lifelong vaccination. Currently many older persons have been vaccinated against selected pathogens during childhood but lack regular booster immunizations. The impact of regular vaccinations when started late in life was analyzed in an open, explorative trial by evaluating the immune response against tetanus and diphtheria in healthy older individuals. 252 persons aged above 60 years received a booster vaccination against tetanus, diphtheria, pertussis and polio and a subcohort (n=87) was recruited to receive a second booster vaccination against tetanus, diphtheria and pertussis 5 years later. The percentage of unprotected individuals at the time of enrollment differed substantially for tetanus (12%) and diphtheria (65%). Despite protective antibody concentrations 4 weeks after the first vaccination in almost all vaccinees, antibodies had again dropped below protective levels in 10% (tetanus) and 45% (diphtheria) of the cohort after 5 years. Protection was restored in almost all vaccinees after the second vaccination. No correlation between tetanus- and diphtheria-specific responses was observed, and antibody concentrations were not associated with age-related changes in the T cell repertoire, inflammatory parameters, or CMV-seropositivity suggesting that there was no general biological “non-responder type.” Post-vaccination antibody concentrations depended on pre-existing plasma cells and B cell memory as indicated by a strong positive relationship between post-vaccination antibodies and pre-vaccination antibodies as well as antibody-secreting cells. In contrast, antigen-specific T cell responses were not or only weakly associated with antibody concentrations. In conclusion, our findings demonstrate that single shot vaccinations against tetanus and/or diphtheria do not lead to long-lasting immunity in many elderly persons despite

Forging Long Shafts On Disks

Science.gov (United States)

Tilghman, Chris; Askey, William; Hopkins, Steven

1989-01-01

Isothermal-forging apparatus produces long shafts integral with disks. Equipment based on modification of conventional isothermal-forging equipment, required stroke cut by more than half. Enables forging of shafts as long as 48 in. (122 cm) on typical modified conventional forging press, otherwise limited to making shafts no longer than 18 in. (46cm). Removable punch, in which forged material cools after plastic deformation, essential novel feature of forging apparatus. Technology used to improve such products as components of gas turbines and turbopumps and of other shaft/disk parts for powerplants, drive trains, or static structures.

Does vaccination ensure protection? Assessing diphtheria and tetanus antibody levels in a population of healthy children: A cross-sectional study.

Science.gov (United States)

Gowin, Ewelina; Wysocki, Jacek; Kałużna, Ewelina; Świątek-Kościelna, Bogna; Wysocka-Leszczyńska, Joanna; Michalak, Michał; Januszkiewicz-Lewandowska, Danuta

2016-12-01

Vaccination effectiveness is proven when the disease does not develop after a patient is exposed to the pathogen. In the case of rare diseases, vaccination effectiveness is assessed by monitoring specific antibody levels in the population. Such recurrent analyses allow the evaluation of vaccination programs. The primary schedule of diphtheria and tetanus vaccinations is similar in various countries, with differences mainly in the number and timing of booster doses. The aim of the study was to assess diphtheria and tetanus antibody concentrations in a population of healthy children.Diphtheria and tetanus antibody levels were analyzed in a group of 324 children aged 18 to 180 months. All children were vaccinated in accordance with the Polish vaccination schedule.Specific antibody concentrations greater than 0.1 IU/mL were considered protective against tetanus or diphtheria. Levels above 1.0 were considered to ensure long-term protection.Protective levels of diphtheria antibodies were found in 229 patients (70.46%), and of tetanus in 306 patients (94.15%). Statistically significant differences were found in tetanus antibody levels in different age groups. Mean concentrations and the percentage of children with high tetanus antibody titers increased with age. No similar correlation was found for diphtheria antibodies. High diphtheria antibody levels co-occurred in 72% of the children with high tetanus antibody levels; 95% of the children with low tetanus antibody levels had low levels of diphtheria antibodies.The percentage of children with protective diphtheria antibody levels is lower than that in the case of tetanus antibodies, both in Poland and abroad, but the high proportion of children without diphtheria protection in Poland is an exception. This is all the more puzzling when taking into account that Polish children are administered a total of 5 doses containing a high concentration of diphtheria toxoid, at intervals shorter than 5 years. The decrease in

Laser shaft alignment measurement model

Science.gov (United States)

Mo, Chang-tao; Chen, Changzheng; Hou, Xiang-lin; Zhang, Guoyu

2007-12-01

Laser beam's track which is on photosensitive surface of the a receiver will be closed curve, when driving shaft and the driven shaft rotate with same angular velocity and rotation direction. The coordinate of arbitrary point which is on the curve is decided by the relative position of two shafts. Basing on the viewpoint, a mathematic model of laser alignment is set up. By using a data acquisition system and a data processing model of laser alignment meter with single laser beam and a detector, and basing on the installation parameter of computer, the state parameter between two shafts can be obtained by more complicated calculation and correction. The correcting data of the four under chassis of the adjusted apparatus moving on the level and the vertical plane can be calculated. This will instruct us to move the apparatus to align the shafts.

Type IV pilins regulate their own expression via direct intramembrane interactions with the sensor kinase PilS.

Science.gov (United States)

Kilmury, Sara L N; Burrows, Lori L

2016-05-24

Type IV pili are important virulence factors for many pathogens, including Pseudomonas aeruginosa Transcription of the major pilin gene-pilA-is controlled by the PilS-PilR two-component system in response to unknown signals. The absence of a periplasmic sensing domain suggested that PilS may sense an intramembrane signal, possibly PilA. We suggest that direct interactions between PilA and PilS in the inner membrane reduce pilA transcription when PilA levels are high. Overexpression in trans of PilA proteins with diverse and/or truncated C termini decreased native pilA transcription, suggesting that the highly conserved N terminus of PilA was the regulatory signal. Point mutations in PilA or PilS that disrupted their interaction prevented autoregulation of pilA transcription. A subset of PilA point mutants retained the ability to interact with PilS but could no longer decrease pilA transcription, suggesting that interaction between the pilin and sensor kinase is necessary but not sufficient for pilA autoregulation. Furthermore, PilS's phosphatase motif was required for the autoregulation of pilA transcription, suggesting that under conditions where PilA is abundant, the PilA-PilS interaction promotes PilR dephosphorylation and thus down-regulation of further pilA transcription. These data reveal a clever bacterial inventory control strategy in which the major subunit of an important P. aeruginosa virulence factor controls its own expression.

Construction of blind shafts with the PVS 3500 planetary full shaft drilling machine

International Nuclear Information System (INIS)

Glogowski, P.; Kolditz, H.

1992-01-01

The PVS 3500 planetary full shaft drilling machine has proved as a prototype in the construction of two blind shafts. The drilling rate of 8 m/shift or 25.6 m 3 /MS is outstanding for the initial use of this drilling machine. Blind shafts were cut from the solid by a dry drilling method for the first time. It opens up the possibility of making available storage boreholes for larger quantities of radioactive waste with low activity and for toxic waste materials. (orig.)

Shaft seal assembly and method

Science.gov (United States)

Keba, John E. (Inventor)

2007-01-01

A pressure-actuated shaft seal assembly and associated method for controlling the flow of fluid adjacent a rotatable shaft are provided. The seal assembly includes one or more seal members that can be adjusted between open and closed positions, for example, according to the rotational speed of the shaft. For example, the seal member can be configured to be adjusted according to a radial pressure differential in a fluid that varies with the rotational speed of the shaft. In addition, in the closed position, each seal member can contact a rotatable member connected to the shaft to form a seal with the rotatable member and prevent fluid from flowing through the assembly. Thus, the seal can be closed at low speeds of operation and opened at high speeds of operation, thereby reducing the heat and wear in the seal assembly while maintaining a sufficient seal during all speeds of operation.

The SSC access shafts calculational study

International Nuclear Information System (INIS)

Baishev, I.S.; Mokhov, N.V.; Toohig, T.E.

1991-06-01

The SSC generic shaft requirements and access spacing are considered elsewhere. The shafts connecting the ground surface with the underground accelerator tunnel deliver to the surface some portion of the radiation created in the tunnel. The radiation safety problem of access shafts consists of two major questions: Does the dose equivalent at the ground surface exceed permissible limits? If it exceeds those limits, what additional shielding measures are required? A few works deal with this problem for high energy machines. This work is an attempt to answer these questions for the basic types of shafts specific to the SSC magnet delivery, utility and personnel shafts using full-scale Monte-Carlo calculations of the entire process from hadronic cascades in the lattice elements to particles scattered in the tunnel, niches, alcoves, shafts and surface bunkers and buildings. 9 refs., 16 figs., 1 tab

Estudo da difteria na cidade do Recife. I. Nota sôbre levantamento de portadores de Corynebacterium diphtheriae no bairro dos Coelhos Survey on diphtheriae carriers in "Bairro dos Coelhos" Recife, Brazil

Directory of Open Access Journals (Sweden)

Dalva A. Mello

1969-06-01

Full Text Available De uma amostra probabilística do bairro dos Coelhos da cidade do Recife, 410 indivíduos foram examinados para verificação de portadores de difteria. Sòmente duas amostras de C. diphtheriae foram isoladas de duas crianças de 8 a 9 anos, as quais não apresentaram sintomatologia compatível com o quadro diftérico.From a limited population living around the University Hospital in Recife, Brazil a randomic sample was examined in order to identify diphtheria carriers. Swabs were made from 410 persons in a house-to-house survey. Two strains of Corynebacterium diphtheriae were isolated from healthy 8 and 9-year old children.

Assessment of Serologic Immunity to Diphtheria-Tetanus-Pertussis After Treatment of Korean Pediatric Hematology and Oncology Patients

Science.gov (United States)

Kwon, Hyo Jin; Lee, Jae-Wook; Chung, Nak-Gyun; Cho, Bin; Kim, Hack-Ki

2012-01-01

The aim of this study was to investigate the diphtheria-tetanus-pertussis antibody titers after antineoplastic treatment and to suggest an appropriate vaccination approach for pediatric hemato-oncologic patients. A total of 146 children with either malignancy in remission after cessation of therapy or bone marrow failure were recruited. All children had received routine immunization including diphtheria-tetanus-acellular pertussis vaccination before diagnosis of cancer. The serologic immunity to diphtheria, tetanus and pertussis was classified as: completely protective, partially protective, or non-protective. Non-protective serum antibody titer for diphtheria, tetanus and pertussis was detected in 6.2%, 11.6%, and 62.3% of patients, respectively, and partial protective serum antibody titer for diphtheria, tetanus and pertussis was seen in 37%, 28.1%, and 8.9% of patients. There was no significant correlation between the severity of immune defect and age, gender or underlying disease. Revaccination after antineoplastic therapy showed significantly higher levels of antibody for each vaccine antigen. Our data indicates that a large proportion of children lacked protective serum concentrations of antibodies against diphtheria, tetanus, and pertussis. This suggests that reimmunization of these patients is necessary after completion of antineoplastic treatment. Also, prospective studies should be undertaken with the aim of devising a common strategy of revaccination. PMID:22219618

A cross-sectional study of tetanus and diphtheria antibody concentrations post vaccination among lung transplant patients compared with healthy individuals.

Science.gov (United States)

Rohde, K A; Cunningham, K C; Henriquez, K M; Nielsen, A R; Worzella, S L; Hayney, M S

2014-12-01

Lung transplant (LuTx) patients are routinely immunized against tetanus and diphtheria. However, few studies have been done to measure serologic immunity in the transplant population. The primary objective of this study was to compare tetanus and diphtheria antibody concentrations in LuTx vs. healthy subjects. Serum was used from an available sample of 111 total individuals (n = 36 healthy; n = 75 LuTx). Tetanus and diphtheria antibody concentrations were measured using an enzyme-linked immunosorbant assay method. A statistically significant difference in both tetanus and diphtheria antibody concentrations was found between the groups. The median concentration of tetanus antibody was higher for healthy individuals compared with the LuTx group (3.2 IU/mL [1.2-5.2 interquartile range {IQR}] vs. 1.3 IU/mL [0.4-2.6 IQR], respectively; P = 0.0001). No difference in time was found since the last tetanus-diphtheria vaccine or tetanus-diphtheria-pertussis vaccine dose between the groups (healthy 76.5 months [16-114 IQR] vs. LuTx 74.5 months [45-118 IQR]; P = 0.44). Tetanus and diphtheria immunizations are recommended for LuTx patients to reduce the risk of infection. Because the LuTx group has lower antibody concentrations, further studies should investigate the possible need for more frequent tetanus and diphtheria boosters. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

30 CFR 56.19106 - Shaft sets.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Shaft sets. 56.19106 Section 56.19106 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE SAFETY AND... Shaft sets. Shaft sets shall be kept in good repair and clean of hazardous material. ...

Immunogenicity and safety of combined adsorbed low-dose diphtheria, tetanus and inactivated poliovirus vaccine (REVAXIS®) versus combined diphtheria, tetanus and inactivated poliovirus vaccine (DT Polio®) given as a booster dose at 6 years of age

Science.gov (United States)

Gajdos, Vincent; Soubeyrand, Benoit; Vidor, Emmanuel; Richard, Patrick; Boyer, Julie; Sadorge, Christine

2011-01-01

This randomized, comparative, phase-IIIb study conducted in France aimed to demonstrate whether seroprotection against diphtheria, tetanus and poliomyelitis 1 month after a single dose of REVAXIS (low-dose diphtheria) is non-inferior to seroprotection 1 month after a single dose of DT Polio (standard-dose diphtheria), both vaccines being given as a second booster to healthy children at 6 years of age. Children were randomly assigned to receive a single intramuscular dose of REVAXIS or DT Polio. Primary endpoints were the 1-month post-booster seroprotection rates for diphtheria, tetanus and poliovirus type-1, -2 and -3 antigens. Secondary endpoints were immunogenicity and safety observations. Of 788 children screened, 760 were randomized: REVAXIS group, 384 children; DT Polio group, 376 children. No relevant difference in demographic characteristics at baseline was observed between REVAXIS and DT Polio groups. Noninferiority of REVAXIS compared with DT Polio for seroprotection was demonstrated against diphtheria (respectively 98.6% and 99.3%), tetanus (respectively 99.6% and 100%) and poliovirus antigens (100% for each types in both groups). No allergic reactions to REVAXIS were reported. A benefit/risk ratio in favor of REVAXIS was suggested by the trend towards a better tolerability of REVAXIS compared with DT Polio regarding the rate of severe solicited injection-site reactions. The results support the use of REVAXIS as a booster at 6 years of age in infants who previously received a three-dose primary series within the first 6 months of life and a first booster including diphtheria, tetanus and poliovirus vaccine(s) given before 2 years of age. PMID:21441781

Shaft and tunnel sealing considerations

International Nuclear Information System (INIS)

Kelsall, P.C.; Shukla, D.K.

1980-01-01

Much of the emphasis of previous repository sealing research has been placed on plugging small diameter boreholes. It is increasingly evident that equal emphasis should now be given to shafts and tunnels which constitute more significant pathways between a repository and the biosphere. The paper discusses differences in requirements for sealing shafts and tunnels as compared with boreholes and the implications for seal design. Consideration is given to a design approach for shaft and tunnel seals based on a multiple component design concept, taking into account the requirements for retrievability of the waste. A work plan is developed for the future studies required to advance shaft and tunnel sealing technology to a level comparable with the existing technology for borehole sealing

30 CFR 57.19106 - Shaft sets.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Shaft sets. 57.19106 Section 57.19106 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE SAFETY AND....19106 Shaft sets. Shaft sets shall be kept in good repair and clean of hazardous material. ...

Re-emergence of diphtheria and pertussis: implications for Nigeria.

Science.gov (United States)

Sadoh, A E; Oladokun, R E

2012-11-26

In the prevaccine era pertussis and diphtheria were responsible for significant morbidity and mortality in children. In the United States of America more than 125,000 cases of diphtheria with 10,000 deaths were reported annually in the 1920s. In the same period about 1.7 million cases of pertussis with 73,000 deaths were also reported. Vaccination against these two diseases has caused remarkable reduction in the morbidity and mortality from these diseases both in developed and developing countries. The initial vaccines were the combined diphtheria toxoid and whole cell pertussis vaccine. The recent reported increases in the incidence of these two diseases in countries, which maintain high childhood vaccination coverage is a source of concern not only to these countries but also for developing countries with weak immunization programmes. Nigeria for example reported 11,281 cases of pertussis, the second highest number of cases worldwide in 2009. Waning immunity in adult and adolescent populations has been reported and epidemiologically, more cases are being reported in adults and adolescents. Also a high proportion of pertussis cases are being reported in infants and most of these infant cases are linked to adult/adolescent sources. Recent approaches to control of these diseases include booster doses of combined diphtheria, tetanus and acellular pertussis vaccine while the cocooning strategy (which is immunizing every person who is likely to have contact with a given infant such as mother, father, grandparents and health care workers) is being used in a number of countries. For developing countries including Nigeria where the capacity for making the diagnosis of both diseases is limited, strengthening of routine immunization as well as diagnostic capacity is imperative. Research to determine current levels of immunity in children, adolescents and adults is required. This will enable the determination of the need for booster doses and the age at which such boosters

Ultrasonic test of highly stressed gear shafts

Energy Technology Data Exchange (ETDEWEB)

Schreiner, T [Siemens AG, Power Generation, KWU, Muelheim (Germany); Heinrich, W [Siemens AG, Power Generation, KWU, Berlin (Germany); Achtzehn, J [Siemens AG, Power Generation, ICVW, Erlangen (Germany); Hensley, H [Siemens Power Generation (Germany)

1999-12-31

In the power plant industry, gears are used for increasingly higher turbine capacities. Efficiency enhancements, particularly for the combined gas and steam turbine process, lead to an increase in stresses, even for high-performance gears. Consequently, the requirements for non-destructive material testing are on the increase as well. At Siemens KWU, high-performance gears are used so far only for gas turbines with lower rating (65 MW) to adapt the gas turbine speed (5413 rpm) to the generator speed (3000 rpm/ 50 Hz or 3600 rpm/60 Hz). The gear train consists of a forged and case-hardened wheel shaft and pinion shaft made of material 17 CrNiMo 6, where the wheel shaft can be either a solid or a hollow shaft. Dimensions are typically 2.3 m length and 1 m diameter. As a rule, pinion shafts are solid. The gear design, calling for an additional torsion shaft turning inside the hollow wheel shaft, can absorb more torsional load surges and is more tolerant of deviations during gear train alignment. This design requires two additional forgings (torsion shaft and hub) and an additional bearing 2 refs.

Ultrasonic test of highly stressed gear shafts

Energy Technology Data Exchange (ETDEWEB)

Schreiner, T. [Siemens AG, Power Generation, KWU, Muelheim (Germany); Heinrich, W. [Siemens AG, Power Generation, KWU, Berlin (Germany); Achtzehn, J. [Siemens AG, Power Generation, ICVW, Erlangen (Germany); Hensley, H. [Siemens Power Generation (Germany)

1998-12-31

In the power plant industry, gears are used for increasingly higher turbine capacities. Efficiency enhancements, particularly for the combined gas and steam turbine process, lead to an increase in stresses, even for high-performance gears. Consequently, the requirements for non-destructive material testing are on the increase as well. At Siemens KWU, high-performance gears are used so far only for gas turbines with lower rating (65 MW) to adapt the gas turbine speed (5413 rpm) to the generator speed (3000 rpm/ 50 Hz or 3600 rpm/60 Hz). The gear train consists of a forged and case-hardened wheel shaft and pinion shaft made of material 17 CrNiMo 6, where the wheel shaft can be either a solid or a hollow shaft. Dimensions are typically 2.3 m length and 1 m diameter. As a rule, pinion shafts are solid. The gear design, calling for an additional torsion shaft turning inside the hollow wheel shaft, can absorb more torsional load surges and is more tolerant of deviations during gear train alignment. This design requires two additional forgings (torsion shaft and hub) and an additional bearing 2 refs.

Technetium-99m labeling and fibronectin binding ability of Corynebacterium diphtheriae; Marcacao de Corynebacterium diphtheriae com Tecnecio-99m e avaliacao da capacidade de ligacao a fibronectina de plasma humano

Energy Technology Data Exchange (ETDEWEB)

Souza, S.M.S.; Nagao, P.E.; Bernardo-Filho, M. [Universidade do Estado do Rio de Janeiro, RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes; Pereira, G.A.; Napoleao, F.; Andrade, A.F.B.; Hirata Junior, R.; Mattos-Guaraldi, A.L. [Universidade do Estado do Rio de Janeiro, RJ (Brazil). Faculdade de Ciencias Medicas

2004-04-15

The use of radionuclides has permitted advances in areas of clinical and scientific knowledge. Several molecules and cells have been labelled with Technetium-99m ({sup 99m}Tc). The stannous chloride (SnCl{sub 2}) has a significant influence on the labeling and stability of {sup 99m}Tc radiotracers. The frequent risk of diphtheria epidemics has intensified interest in the virulence factors of Corynebacterium diphtheriae. Although studies have looked at potential adhesins including haemagglutinins and exposed sugar residues, the molecular basis of mechanisms of adherence remains unclear. Adherence of pathogens to mammalian tissues may be mediated by fibronectin (FN) found in body fluids, matrix of connective tissues, and cell surfaces. In the present study we evaluated the binding ability to human plasma FN by {sup 99m}Tc labeled-C.diphtheriae. Due to adverse effects of stannous ions, microorganisms were submitted to survival and filamentation induction assays. Data showed a dose dependent susceptibility to SnCl{sub 2} bactericidal effects. Cell filamentation was observed for concentrations of SnCl{sub 2} > 110 {mu}g/ml. Adherence levels of {sup 99m}Tc labelled 241strain to coverslips coated with 20 {mu}g/ml FN were higher (P = 0.0037) than coated with bovine serum albumin. FN binding by the sucrose fermenting 241 C. diphtheriae strain (8.9% + 2.6) was significantly lower (P=0.0139) than Staphylococcus aureus Cowan I strain (34.1% {+-} 1.2). Therefore, bacterial {sup 99m}Tc labeling represents an additional tool that may contribute to the comprehension of C. diphtheriae interactions with host receptors such as FN that act as biological organizers by holding bacterial cells in position and guiding their migration. (author)

Public health action following an outbreak of toxigenic cutaneous diphtheria in an Auckland refugee resettlement centre.

Science.gov (United States)

Reynolds, Gary E; Saunders, Helen; Matson, Angela; O'Kane, Fiona; Roberts, Sally A; Singh, Salvin K; Voss, Lesley M; Kiedrzynski, Tomasz

2016-12-24

Global forced displacement has climbed to unprecedented levels due largely to regional conflict. Degraded public health services leave displaced people vulnerable to multiple environmental and infectious hazards including vaccine preventable disease. While diphtheria is rarely notified in New Zealand, a 2 person outbreak of cutaneous diphtheria occurred in refugees from Afghanistan in February 2015 at the refugee resettlement centre in Auckland. Both cases had uncertain immunisation status. The index case presented with a scalp lesion during routine health screen and toxigenic Corynebacterium diphtheriae was isolated. A secondary case of cutaneous diphtheria and an asymptomatic carrier were identified from skin and throat swabs. The 2 cases and 1 carrier were placed in consented restriction until antibiotic treatment and 2 clearance swabs were available. A total of 164 contacts were identified from within the same hostel accommodation as well as staff working in the refugee centre. All high risk contacts (n=101) were swabbed (throat, nasopharynx and open skin lesions) to assess C. diphtheriae carriage status. Chemoprophylaxis was administered (1 dose of intramuscular benzathine penicillin or 10 days of oral erythromycin) and diphtheria toxoid-containing vaccine offered regardless of immunisation status. Suspected cases were restricted on daily monitoring until swab clearance. A group of 49 low risk contacts were also offered vaccination. Results suggest a significant public health effort was required for a disease rarely seen in New Zealand. In light of increased worldwide forced displacement, similar outbreaks could occur and require a rigorous public health framework for management.

High pressure shaft seal

International Nuclear Information System (INIS)

Martinson, A.R.; Rogers, V.D.

1980-01-01

In relation to reactor primary coolant pumps, mechanical seal assembly for a pump shaft is disclosed which features a rotating seal ring mounting system which utilizes a rigid support ring loaded through narrow annular projections in combination with centering non-sealing O-rings which effectively isolate the rotating seal ring from temperature and pressure transients while securely positioning the ring to adjacent parts. A stationary seal ring mounting configuration allows the stationary seal ring freedom of motion to follow shaft axial movement up to 3/4 of an inch and shaft tilt about the pump axis without any change in the hydraulic or pressure loading on the stationary seal ring or its carrier. (author)

Induction of diphtheria toxin-resistant mutants in human cells by ultraviolet light

International Nuclear Information System (INIS)

Rocchi, P.; Ferreri, A.M.; Capucci, A.; Prodi, G.

1981-01-01

Stable spontaneous mutants resistant to the protein synthesis inhibitor diphtheria toxin (DT) have been selected in human cell line EUE at a very low frequency (less than 8 x 10(-6)). U.v.-induced mutation has been quantitatively measured: treatment of cells with u.v. light increased the frequencies of diphtheria toxin resistant (DTr) mutants up to 1000-fold. The maximum recovery of DTr mutants was observed after a short expression period, for all u.v. doses tested, and was followed by a decrease in mutation frequency on subsequent passages

Induction of diphtheria toxin-resistant mutants in human cells by ultraviolet light

International Nuclear Information System (INIS)

Rocchi, P.; Ferreri, A.M.; Capucci, A.; Prodi, G.

1981-01-01

Stable spontaneous mutants resistant to the protein synthesis inhibitor diphtheria toxin (DT) have been selected in human cell line EUE at a very low frequency ( -6 ). U.v.-induced mutation has been quantitatively measured: treatment of cells with u.v. light increased the frequencies of diphtheria toxin resistant (DTsup(r)) mutants up to 1000-fold. The maximum recovery of DTsup(r) mutants was observed after a short expression period, for all u.v. doses tested, and was followed by a decrease in mutation frequency on subsequent passages. (author)

Screening for Corynebacterium diphtheriae and Corynebacterium ulcerans in patients with upper respiratory tract infections 2007-2008: a multicentre European study.

LENUS (Irish Health Repository)

Wagner, K S

2011-04-01

Diphtheria is now rare in most European countries but, when cases do arise, the case fatality rate is high (5-10%). Because few countries continue to routinely screen for the causative organisms of diphtheria, the extent to which they are circulating amongst different European populations is largely unknown. During 2007-2008, ten European countries each screened between 968 and 8551 throat swabs from patients with upper respiratory tract infections. Six toxigenic strains of Corynebacterium diphtheriae were identified: two from symptomatic patients in Latvia (the country with the highest reported incidence of diphtheria in the European Union) and four from Lithuania (two cases, two carriers); the last reported case of diphtheria in Lithuania was in 2002. Carriage rates of non-toxigenic organisms ranged from 0 (Bulgaria, Finland, Greece, Ireland, Italy) to 4.0 per 1000 (95% CI 2.0-7.1) in Turkey. A total of 28 non-toxigenic strains were identified during the study (26 C. diphtheriae, one Corynebacterium ulcerans, one Corynebacterium pseudotuberculosis). The non-toxigenic C. ulcerans strain was isolated from the UK, the country with the highest reported incidence of cases due to C. ulcerans. Of the eleven ribotypes detected, Cluj was seen most frequently in the non-toxigenic isolates and, amongst toxigenic isolates, the major epidemic clone, Sankt-Petersburg, is still in circulation. Isolation of toxigenic C. diphtheriae and non-toxigenic C. diphtheriae and C. ulcerans in highly-vaccinated populations highlights the need to maintain microbiological surveillance, laboratory expertise and an awareness of these organisms amongst public health specialists, microbiologists and clinicians.

Large shaft development test plan

International Nuclear Information System (INIS)

Krug, A.D.

1984-03-01

This test plan proposes the conduct of shaft liner tests as part of the large shaft development test proposed for the Hanford Site in support of the repository development program. The objectives of these tests are to develop techniques for measuring liner alignment (straightness), both construction assembly alignment and downhole cumulative alignment, and to assess the alignment information as a real time feedback to aid the installation procedure. The test plan is based upon installing a 16 foot ID shaft liner into a 20 foot diameter shaft to a depth of 1000 feet. This test plan is considered to be preliminary in that it was prepared as input for the decision to determine if development testing is required in this area. Should the decision be made to proceed with development testing, this test plan shall be updated and revised. 6 refs., 2 figs

Torsion of a growing shaft

Directory of Open Access Journals (Sweden)

Alexander V. Manzhirov

2017-12-01

Full Text Available The torsion of a shaft by rigid disks is considered. The shaft has the form of circular cylinder. Two rigid disks are attached to its end faces. The process of continuous growth of such shaft under the influence of twisting torques applied to the disks is studied. Dual series equations which reflect the mathematical content of the problem at the different stages of the growing process are derived and solved. Results of the numerical analysis and singularities of the qualitative mechanical behaviour of the fundamental characteristics are discussed.

Boundary integral method for torsion of composite shafts

International Nuclear Information System (INIS)

Chou, S.I.; Mohr, J.A.

1987-01-01

The Saint-Venant torsion problem for homogeneous shafts with simply or multiply-connected regions has received a great deal of attention in the past. However, because of the mathematical difficulties inherent in the problem, very few problems of torsion of shafts with composite cross sections have been solved analytically. Muskhelishvili (1963) studied the torsion problem for shafts with cross sections having several solid inclusions surrounded by an elastic material. The problem of a circular shaft reinforced by a non-concentric round inclusion, a rectangular shaft composed of two rectangular parts made of different materials were solved. In this paper, a boundary integral equation method, which can be used to solve problems more complex than those considered by Katsikadelis et. al., is developed. Square shaft with two dissimilar rectangular parts, square shaft with a square inclusion are solved and the results compared with those given in the reference cited above. Finally, a square shaft composed of two rectangular parts with circular inclusion is solved. (orig./GL)

Your Child's Immunizations: Diphtheria, Tetanus & Pertussis Vaccine (DTaP)

Science.gov (United States)

... Safe Videos for Educators Search English Español Your Child's Immunizations: Diphtheria, Tetanus & Pertussis Vaccine (DTaP) KidsHealth / For ... child outweigh the potential risks. Caring for Your Child After DTaP Immunization Your child may have a ...

Td Vaccine (Tetanus and Diphtheria): What You Need to Know

Science.gov (United States)

VACCINE INFORMATION STATEMENT Td Vaccine (Tetanus and Diphtheria) What You Need to Know Many Vaccine Information Statements are available in Spanish and other languages. See www. immunize. org/ vis Hojas de ...

Femoral shaft fractures

International Nuclear Information System (INIS)

Bender, C.E.; Campbell, D.C. II

1985-01-01

The femur is the longest, largest, and strongest bone in the body. Because of its length, width, and role as primary weight-bearing bone, it must tolerate the extremes of axial loading and angulatory stresses. Massive musculature envelopes the femur. This masculature provides abundant blood supply to the bone, which also allows great potential for healing. Thus, the most significant problem relating to femoral shaft fractures is not healing, but restoration of bone length and alignment so that the femoral shaft will tolerate the functional stresses demanded of it

Mutant with diphtheria toxin receptor and acidification function but defective in entry of toxin

International Nuclear Information System (INIS)

Kohno, Kenji; Hayes, H.; Mekada, Eisuke; Uchida, Tsuyoshi

1987-01-01

A mutant of Chinese hamster ovary cells, GE1, that is highly resistant to diphtheria toxin was isolated. The mutant contains 50% ADP-ribosylatable elongation factor 2, but its protein synthesis was not inhibited by the toxin even at concentrations above 100 μg/ml. 125 I-labeled diphtheria toxin was associated with GE1 cells as well as with the parent cells but did not block protein synthesis of GE1 cells even when the cells were exposed to low pH in the presence or absence of NH 4 Cl. The infections of GE1 cells and the parent cells by vesicular stomatitis virus were similar. GE1 cells were cross-resistant to Pseudomonas aeruginosa exotoxin A and so were about 1,000 times more resistant to this toxin than the parent cells. Hybrids of GE1 cells and the parent cells or mutant cells lacking a functional receptor were more sensitive to diphtheria toxin than GE1 cells. These results suggest that entry of diphtheria toxin into cells requires a cellular factor(s) in addition to those involved in receptor function and acidification of endosomes and that GE1 cells do not express this cellular factor. This character is recessive in GE1 cells

Pilus Biogenesis in Lactococcus lactis: Molecular Characterization and Role in Aggregation and Biofilm Formation

Science.gov (United States)

Oxaran, Virginie; Ledue-Clier, Florence; Dieye, Yakhya; Herry, Jean-Marie; Péchoux, Christine; Meylheuc, Thierry; Briandet, Romain; Juillard, Vincent; Piard, Jean-Christophe

2012-01-01

The genome of Lactococcus lactis strain IL1403 harbors a putative pilus biogenesis cluster consisting of a sortase C gene flanked by 3 LPxTG protein encoding genes (yhgD, yhgE, and yhhB), called here pil. However, pili were not detected under standard growth conditions. Over-expression of the pil operon resulted in production and display of pili on the surface of lactococci. Functional analysis of the pilus biogenesis machinery indicated that the pilus shaft is formed by oligomers of the YhgE pilin, that the pilus cap is formed by the YhgD pilin and that YhhB is the basal pilin allowing the tethering of the pilus fibers to the cell wall. Oligomerization of pilin subunits was catalyzed by sortase C while anchoring of pili to the cell wall was mediated by sortase A. Piliated L. lactis cells exhibited an auto-aggregation phenotype in liquid cultures, which was attributed to the polymerization of major pilin, YhgE. The piliated lactococci formed thicker, more aerial biofilms compared to those produced by non-piliated bacteria. This phenotype was attributed to oligomers of YhgE. This study provides the first dissection of the pilus biogenesis machinery in a non-pathogenic Gram-positive bacterium. Analysis of natural lactococci isolates from clinical and vegetal environments showed pili production under standard growth conditions. The identification of functional pili in lactococci suggests that the changes they promote in aggregation and biofilm formation may be important for the natural lifestyle as well as for applications in which these bacteria are used. PMID:23236417

Shaft placement in a bedded salt repository

International Nuclear Information System (INIS)

Klasi, M.L.

1982-10-01

Preferred shaft pillar sizes and shaft locations were determined with respect to the induced thermal stresses in a generic bedded salt repository at a depth of 610 m with a gross thermal loading of 14.8 W/m 2 . The model assumes isotropic material properties, plane strain and linear elastic behavior. Various shaft locations were analyzed over a 25 year period. The thermal results show that for this time span, the stratigraphy is unimportant except for the region immediately adjacent to the repository. The thermomechanical results show that for the given repository depth of 610 m, a minimum central shaft pillar radius of 244 m is required to equal the material strength in the barrier pillar. An assumed constant stress and constant temperature distribution creep model of the central shaft region adjacent to the repository conservatively overestimates a creep closure of 310 mm in a 6.1 m diameter centrally-located shaft

Connect-disconnect coupling for preadjusted rigid shafts

Science.gov (United States)

Bajkowski, F. W.; Holmberg, A.

1969-01-01

Coupling device enables a rigid shaft to be connected to or disconnected from a fixed base without disturbing the point of adjustment of the shaft in a socket or causing the shaft to rotate. The coupling consists of an externally threaded, internally slotted boss extending from the fixed base.

Diphtheria Antibodies and T lymphocyte Counts in Patients Infected with HIV-1

Directory of Open Access Journals (Sweden)

Francisco A. B. Speranza

2012-09-01

Full Text Available We assessed the IgG levels anti-diphtheria (D-Ab and T cell counts (CD4+ and CD8+ in HIV-1 infected subjects undergoing or not highly active antiretroviral therapy (HAART. Approximately 70% of all HIV-1 patients were unprotected against diphtheria. There were no differences in D-Ab according to CD4 counts. Untreated patients had higher D-Ab (geometric mean of 0.62 IU/ml than HAART-patients (geometric mean of 0.39 IU/ml. The data indicated the necessity of keeping all HIV-1 patients up-to-date with their vaccination.

Shaft MisalignmentDetectionusing Stator Current Monitoring

OpenAIRE

Alok Kumar Verma, Somnath Sarangi and M.H. Kolekar

2013-01-01

This paper inspects the misaligned of shaft by usingdiagnostic medium such as current and vibration.Misalignments in machines can cause decrease inefficiency and in the long-run it may cause failurebecause of unnecessary vibration, stress on motor,bearings and short-circuiting in stator and rotorwindings.In this study, authors investigate the onsetof instability on a shaft mounted on journal bearings.Shaft displacement and stator current samples duringmachine run up under misaligned condition...

SINGLE CHAIN VARIABLE FRAGMENTS OF ANTIBODIES AGAINST DIPHTHERIA TOXIN B-SUBUNIT ISOLATED FROM PHAGE DISPLAY HUMAN ANTIBODY LIBRARY

Directory of Open Access Journals (Sweden)

Oliinyk O. S.

2014-02-01

Full Text Available Diphtheria toxin is an exoantigen of Corynebacterium diphtheriae that inhibits protein synthesis and kills sensitive cells. The aim of this study was to obtain human recombinant single-chain variable fragment (scFv antibodies against receptor-binding B subunit of diphtheria toxin. 12 specific clones were selected after three rounds of a phage display naїve (unimmunized human antibody library against recombinant B-subunit. scFv DNA inserts from these 12 clones were digested with MvaI, and 6 unique restriction patterns were found. Single-chain antibodies were expressed in Escherichia coli XL1-blue. The recombinant proteins were characterized by immunoblotting of bacterial extracts and detection with an anti-E-tag antibody. The toxin B-subunit-binding function of the single-chain antibody was shown by ELISA. The affinity constants for different clones were found to be from 106 to 108 М–1. Due to the fact, that these antibody fragments recognized epitopes in the receptor-binding Bsubunit of diphtheria toxin, further studies are interesting to evaluate their toxin neutralization properties and potential for therapeutic applications. Obtained scFv-antibodies can also be used for detection and investigation of biological properties of diphtheria toxin.

Effective humoral immunity against diphtheria and tetanus in patients with systemic lupus erythematosus or myasthenia gravis.

Science.gov (United States)

Csuka, Dorottya; Czirják, László; Hóbor, Renáta; Illes, Zsolt; Bánáti, Miklós; Rajczy, Katalin; Tordai, Attila; Füst, George

2013-07-01

Controversy exists about the effectiveness of vaccine-induced immune response in patients with immunoregulatory disorders. Our aim was to determine the antibody titers to diphtheria and tetanus in patients with either of two autoimmune diseases. 279 patients with SLE (205 females, aged 45.0 ± 13.8 years), 158 patients with myasthenia gravis (MG) (101 females, aged 55 ± 18.7 years) and 208 healthy subjects (122 females, aged 48 ± 14.6 years) were enrolled. Serum concentrations of diphtheria-antitoxin-IgG (A-DIPHTH) and tetanus-antitoxoid-IgG (A-TET) were determined with ELISA. Equal proportions of healthy subjects, as well as patients with SLE or MG exhibited proper antibody responses and immune protection against diphtheria and tetanus. In all three test groups, serum concentration of A-DIPHTH decreased significantly (p60-years-old) subjects. There were no significant differences among the groups in the age-related changes of A-TET and A-DIPHTH except that in diphtheria and tetanus infections in patients with SLE or MG is comparable to the healthy population. Copyright © 2013 Elsevier Ltd. All rights reserved.

Update of 1972 status report on deep shaft studies

International Nuclear Information System (INIS)

1976-09-01

The following aspects of shaft sinking are considered: the effects of geology, factors affecting shaft size, the conventional shaft sinking techniques and the newer mechanized methods, several representative or difficult shafts, and certain long-term problems and solutions

Fatal diphtheria myocarditis in a 3-year-old girl-related to late availability and administration of antitoxin?

NARCIS (Netherlands)

Van Damme, Karlijn; Peeters, Natasja; Jorens, Philippe G; Boiy, Tine; Deplancke, Marjan; Audiens, Hilde; Wojciechowski, Marek; De Dooy, Jozef; Te Wierik, Margreet; Vlieghe, Erika

2017-01-01

Sporadic cases of diphtheria are very rare throughout Europe. A 3-year-old incompletely vaccinated girl was admitted with pharyngotonsillitis caused by diphtheria. On day 9 of her illness, renal and cardiac failure with a third-degree AV-block occurred. Unfortunately, she died within 36 h of

Diphtheria in a 7-year-old child in north-eastern Nigeria - management in a resource-poor setting.

Science.gov (United States)

Goni, Baba Waru; Gofama, Mustapha M; Lawan, Gana M; Haruna, Yusuph; Bukar, Bakki; Musa, Kida I

2014-01-01

We report a case of a 7-year-old unimmunized child who presented with a 2 week history of nasal quality speech, hoarseness of the voice, regurgitation of feeds, and unstable gait. He had a previous history of fever, severe sore throat and bloody nasal discharge. A throat swab was negative for Corynebacterium diphtheria; however, he had received antibiotics at a primary care clinic prior to presentation. A clinical diagnosis of diphtheria with neurologic complication was made and the child was started on oral erythromycin, nasogastric tube feeding and daily physiotherapy, following which he improved. We did not prescribe diphtheria anti-toxin because of its unavailability.

Incidence and epidemiology of tibial shaft fractures.

Science.gov (United States)

Larsen, Peter; Elsoe, Rasmus; Hansen, Sandra Hope; Graven-Nielsen, Thomas; Laessoe, Uffe; Rasmussen, Sten

2015-04-01

The literature lacks recent population-based epidemiology studies of the incidence, trauma mechanism and fracture classification of tibial shaft fractures. The purpose of this study was to provide up-to-date information on the incidence of tibial shaft fractures in a large and complete population and report the distribution of fracture classification, trauma mechanism and patient baseline demographics. Retrospective reviews of clinical and radiological records. A total of 196 patients were treated for 198 tibial shaft fractures in the years 2009 and 2010. The mean age at time of fracture was 38.5 (21.2SD) years. The incidence of tibial shaft fracture was 16.9/100,000/year. Males have the highest incidence of 21.5/100,000/year and present with the highest frequency between the age of 10 and 20, whereas women have a frequency of 12.3/100,000/year and have the highest frequency between the age of 30 and 40. AO-type 42-A1 was the most common fracture type, representing 34% of all tibial shaft fractures. The majority of tibial shaft fractures occur during walking, indoor activity and sports. The distribution among genders shows that males present a higher frequency of fractures while participating in sports activities and walking. Women present the highest frequency of fractures while walking and during indoor activities. This study shows an incidence of 16.9/100,000/year for tibial shaft fractures. AO-type 42-A1 was the most common fracture type, representing 34% of all tibial shaft fractures. Copyright © 2015 Elsevier Ltd. All rights reserved.

Geotechnical instrumentation for repository shafts

International Nuclear Information System (INIS)

Lentell, R.L.; Byrne, J.

1993-01-01

The US Congress passed the Nuclear Waste Policy Act in 1980, which required that three distinctly different geologic media be investigated as potential candidate sites for the permanent disposal of high-level nuclear waste. The three media that were selected for study were basalt (WA), salt (TX, LA, MS, UT), and tuff (NV). Preliminary Exploratory Shaft Facilities (ESF) designs were prepared for seven candidate salt sites, including bedded and domal salt environments. A bedded-salt site was selected in Deaf Smith County, TX for detailed site characterization studies and ESF Final Design. Although Congress terminated the Salt Repository Program in 1988, Final Design for the Deaf Smith ESF was completed, and much of the design rationale can be applied to subsequent deep repository shafts. This paper presents the rationale for the geotechnical instrumentation that was designed for construction and operational performance monitoring of the deep shafts of the in-situ test facility. The instrumentation design described herein can be used as a general framework in designing subsequent instrumentation programs for future high-level nuclear waste repository shafts

Hair Shaft Abnormality in Children: a Narrative Review

Directory of Open Access Journals (Sweden)

Ghasem Rahmatpour Rokni

2017-08-01

Full Text Available Background Hair is an ectodermal structure, and its formation is regulated by master genes important in embryology. Hair shaft consists of three major regions: the medulla, cortex and cuticle. Hair shaft abnormality will divide structural hair abnormalities into two broad categories - those associated with increased hair fragility and those not associated with increased hair fragility. We conducted a review study to assess hair shaft abnormality in children. Materials and Methods We conducted a review of all papers published on hair shaft abnormalities. A literature search was performed using PubMed, Scopus and Google Scholar on papers publish from 1990 to 2016. The search terms were: hair shaft abnormality, Hair loss, Hair fragility. All abstracts and full text English-language articles were studied. Results While common developmental and structural features are shared in hair follicles and hair shafts. Anomalies of the hair shaft are separated into those with and those without increased hair fragility. Conclusion Although hair has no vital function, it may serve as an indicator for human health. Clinical and morphological hair abnormalities can be clues to specific complex disorders. Hair shaft abnormalities can be inherited or acquired, can reflect a local problem or a systemic disease.

Structure of Streptococcus agalactiae tip pilin GBS104: a model for GBS pili assembly and host interactions

Energy Technology Data Exchange (ETDEWEB)

Krishnan, Vengadesan [UNESCO Regional Centre for Biotechnology (RCB), Gurgaon 122 016, Haryana (India); Dwivedi, Prabhat [University of Texas Health Science Center, Houston, TX 77030 (United States); Kim, Brandon J. [San Diego State University, 5500 Campanile Drive, San Diego, CA 92182 (United States); Samal, Alexandra; Macon, Kevin [University of Alabama at Birmingham, Birmingham, AL 35294 (United States); Ma, Xin; Mishra, Arunima [University of Texas Health Science Center, Houston, TX 77030 (United States); Doran, Kelly S. [San Diego State University, 5500 Campanile Drive, San Diego, CA 92182 (United States); Ton-That, Hung [University of Texas Health Science Center, Houston, TX 77030 (United States); Narayana, Sthanam V. L., E-mail: narayana@uab.edu [University of Alabama at Birmingham, Birmingham, AL 35294 (United States); UNESCO Regional Centre for Biotechnology (RCB), Gurgaon 122 016, Haryana (India)

2013-06-01

The crystal structure of a 75 kDa central fragment of GBS104, a tip pilin from the 2063V/R strain of Streptococcus agalactiae (group B streptococcus; GBS), is reported. The crystal structure of a 75 kDa central fragment of GBS104, a tip pilin from the 2063V/R strain of Streptococcus agalactiae (group B streptococcus; GBS), is reported. In addition, a homology model of the remaining two domains of GBS104 was built and a model of full-length GBS104 was generated by combining the homology model (the N1 and N4 domains) and the crystal structure of the 75 kDa fragment (the N2 and N3 domains). This rod-shaped GBS104 model is constructed of three IgG-like domains (the N1, N2 and N4 domains) and one vWFA-like domain (the N3 domain). The N1 and N2 domains of GBS104 are assembled with distinct and remote segments contributed by the N- and C-termini. The metal-binding site in the N3 domain of GBS104 is in the closed/low-affinity conformation. Interestingly, this domain hosts two long arms that project away from the metal-binding site. Using site-directed mutagenesis, two cysteine residues that lock the N3 domain of GBS104 into the open/high-affinity conformation were introduced. Both wild-type and disulfide-locked recombinant proteins were tested for binding to extracellular matrix proteins such as collagen, fibronectin, fibrinogen and laminin, and an increase in fibronectin binding affinity was identified for the disulfide-locked N3 domain, suggesting that induced conformational changes may play a possible role in receptor binding.

Structure of Streptococcus agalactiae tip pilin GBS104: a model for GBS pili assembly and host interactions

International Nuclear Information System (INIS)

Krishnan, Vengadesan; Dwivedi, Prabhat; Kim, Brandon J.; Samal, Alexandra; Macon, Kevin; Ma, Xin; Mishra, Arunima; Doran, Kelly S.; Ton-That, Hung; Narayana, Sthanam V. L.

2013-01-01

The crystal structure of a 75 kDa central fragment of GBS104, a tip pilin from the 2063V/R strain of Streptococcus agalactiae (group B streptococcus; GBS), is reported. The crystal structure of a 75 kDa central fragment of GBS104, a tip pilin from the 2063V/R strain of Streptococcus agalactiae (group B streptococcus; GBS), is reported. In addition, a homology model of the remaining two domains of GBS104 was built and a model of full-length GBS104 was generated by combining the homology model (the N1 and N4 domains) and the crystal structure of the 75 kDa fragment (the N2 and N3 domains). This rod-shaped GBS104 model is constructed of three IgG-like domains (the N1, N2 and N4 domains) and one vWFA-like domain (the N3 domain). The N1 and N2 domains of GBS104 are assembled with distinct and remote segments contributed by the N- and C-termini. The metal-binding site in the N3 domain of GBS104 is in the closed/low-affinity conformation. Interestingly, this domain hosts two long arms that project away from the metal-binding site. Using site-directed mutagenesis, two cysteine residues that lock the N3 domain of GBS104 into the open/high-affinity conformation were introduced. Both wild-type and disulfide-locked recombinant proteins were tested for binding to extracellular matrix proteins such as collagen, fibronectin, fibrinogen and laminin, and an increase in fibronectin binding affinity was identified for the disulfide-locked N3 domain, suggesting that induced conformational changes may play a possible role in receptor binding

Immunity against diphtheria and tetanus in human immunodeficiency virus-infected Danish men born 1950-59

DEFF Research Database (Denmark)

Kurtzhals, J A; Kjeldsen, K; Heron, I

1992-01-01

To evaluate the possible need for vaccination against diphtheria and tetanus of patients infected with the human immunodeficiency virus (HIV), antibodies were measured in blood samples from 78 Danish HIV-infected men, born 1950-59, who could be expected to have received primary vaccination before...... they contracted the HIV infection. No patients (95% confidence interval: 0-4) had tetanus antibodies below the protective level, whereas 24 of the 78 patients (16-33) were unprotected against diphtheria. In the background population of the same age group and sex, 5% and 10% have been found unprotected against...... tetanus and diphtheria, respectively. No relationship between disease stages and antibody levels could be found. Neither was there any difference between patients with normal and reduced numbers of CD4+ lymphocytes. From 25 patients two blood samples were taken at an interval of at least one year. Anti...

Reliability assessment of underground shaft closure

International Nuclear Information System (INIS)

Fossum, A.F.; Munson, D.E.

1994-01-01

The intent of the WIPP, being constructed in the bedded geologic salt deposits of Southeastern New Mexico, is to provide the technological basis for the safe disposal of radioactive Transuranic (TRU) wastes generated by the defense programs of the United States. In determining this technological basis, advanced reliability and structural analysis techniques are used to determine the probability of time-to-closure of a hypothetical underground shaft located in an argillaceous salt formation and filled with compacted crushed salt. Before being filled with crushed salt for sealing, the shaft provides access to an underground facility. Reliable closure of the shaft depends upon the sealing of the shaft through creep closure and recompaction of crushed backfill. Appropriate methods are demonstrated to calculate cumulative distribution functions of the closure based on laboratory determined random variable uncertainty in salt creep properties

A case of respiratory toxigenic diphtheria: contact tracing results and considerations following a 30-year disease-free interval, Catalonia, Spain, 2015.

Science.gov (United States)

Jané, Mireia; Vidal, Maria José; Camps, Neus; Campins, Magda; Martínez, Ana; Balcells, Joan; Martin-Gomez, Maria Teresa; Bassets, Gloria; Herrera-León, Silvia; Foguet, Anton; Maresma, Mar; Follia, Nuria; Uriona, Sonia; Pumarola, Tomàs

2018-03-01

In May 2015, following a 30-year diphtheria-free interval in Catalonia, an unvaccinated 6-year-old child was diagnosed with diphtheria caused by toxigenic Corynebacterium diphtheriae . After a difficult search for equine-derived diphtheria antitoxin (DAT), the child received the DAT 4 days later but died at the end of June. Two hundred and seventeen contacts were identified in relation to the index case, and their vaccination statuses were analysed, updated and completed. Of these, 140 contacts underwent physical examination and throat swabs were taken from them for analysis. Results were positive for toxigenic C. diphtheriae in 10 contacts; nine were asymptomatic vaccinated children who had been in contact with the index case and one was a parent of one of the nine children. Active surveillance of the 217 contacts was initiated by healthcare workers from hospitals and primary healthcare centres, together with public health epidemiological support. Lack of availability of DAT was an issue in our case. Such lack could be circumvented by the implementation of an international fast-track procedure to obtain it in a timely manner. Maintaining primary vaccination coverage for children and increasing booster-dose immunisation against diphtheria in the adult population is of key importance.

Rotating Shaft Tilt Angle Measurement Using an Inclinometer

Science.gov (United States)

Luo, Jun; Wang, Zhiqian; Shen, Chengwu; Wen, Zhuoman; Liu, Shaojin; Cai, Sheng; Li, Jianrong

2015-10-01

This paper describes a novel measurement method to accurately measure the rotating shaft tilt angle of rotating machine for alignment or compensation using a dual-axis inclinometer. A model of the rotating shaft tilt angle measurement is established using a dual-axis inclinometer based on the designed mechanical structure, and the calculation equation between the rotating shaft tilt angle and the inclinometer axes outputs is derived under the condition that the inclinometer axes are perpendicular to the rotating shaft. The reversal measurement method is applied to decrease the effect of inclinometer drifts caused by temperature, to eliminate inclinometer and rotating shaft mechanical error and inclinometer systematic error to attain high measurement accuracy. The uncertainty estimation shows that the accuracy of rotating shaft tilt angle measurement depends mainly on the inclinometer uncertainty and its uncertainty is almost the same as the inclinometer uncertainty in the simulation. The experimental results indicate that measurement time is 4 seconds; the range of rotating shaft tilt angle is 0.002° and its standard deviation is 0.0006° using NS-5/P2 inclinometer, whose precision and resolution are ±0.01° and 0.0005°, respectively.

Rotating Shaft Tilt Angle Measurement Using an Inclinometer

Directory of Open Access Journals (Sweden)

Luo Jun

2015-10-01

Full Text Available This paper describes a novel measurement method to accurately measure the rotating shaft tilt angle of rotating machine for alignment or compensation using a dual-axis inclinometer. A model of the rotating shaft tilt angle measurement is established using a dual-axis inclinometer based on the designed mechanical structure, and the calculation equation between the rotating shaft tilt angle and the inclinometer axes outputs is derived under the condition that the inclinometer axes are perpendicular to the rotating shaft. The reversal measurement method is applied to decrease the effect of inclinometer drifts caused by temperature, to eliminate inclinometer and rotating shaft mechanical error and inclinometer systematic error to attain high measurement accuracy. The uncertainty estimation shows that the accuracy of rotating shaft tilt angle measurement depends mainly on the inclinometer uncertainty and its uncertainty is almost the same as the inclinometer uncertainty in the simulation. The experimental results indicate that measurement time is 4 seconds; the range of rotating shaft tilt angle is 0.002° and its standard deviation is 0.0006° using NS-5/P2 inclinometer, whose precision and resolution are ±0.01° and 0.0005°, respectively.

FRACTURE SHAFT HUMERUS: INTERLOCKING

Directory of Open Access Journals (Sweden)

Deepak Kaladagi

2014-12-01

Full Text Available BACKGROUND: The incidence of humeral fracture has significantly increased during the present years due to the population growth and road traffic, domestic, industrial, automobile accidents & disasters like tsunami, earthquakes, head-on collisions, polytrauma etc. In order to achieve a stable fixation followed by early mobilization, numerous surgical implants have been devised. PURPOSE: The purpose of this study is to analyze the results of intramedullary fixation of proximal 2/3rd humeral shaft fractures using an unreamed interlocking intramedullary nail. INTRODUCTION: In 40 skeletally matured patients with fracture shaft of humerus admitted in our hospital, we used unreamed antegrade interlocking nails. MATERIAL: We carried out a prospective analysis of 40 patients randomly selected between 2001 to 2014 who were operated at JNMC Belgaum, MMC Mysore & Navodaya Medical College, Raichur. All cases were either RTAs, Domestic, Industrial, automobile accidents & also other modes of injury. METHOD: Routine investigations with pre-anaesthetic check-up & good quality X-rays of both sides of humerus was taken. Time of surgery ranged from 5-10 days from the time of admission. Only upper 1/3rd & middle 1/3rd humeral shaft fractures were included in the study. In all the cases antegrade locked unreamed humeral nails were inserted under C-arm. Patient was placed in supine position & the shoulder was kept elevated by placing a sandbag under the scapula. In all patients incision taken from tip of acromion to 3cm over deltoid longitudinally. Postoperatively sling applied with wrist & shoulder movements started after 24 hours. All the patients ranged between the age of 21-50 years. RESULTS: Total 40 patients were operated. Maximum fracture site were in the middle third- 76%, 14% upper 1/3rd. All 40 patients achieved union. The average time of union was 8-10 weeks. All patients regained full range of movements except in few cases, where there was shoulder

Long-term brine migration through an engineered shaft seal system

International Nuclear Information System (INIS)

Fryar, D.G.; Beach, J.A.; Kelley, V.A.; Knowles, M.K.

1997-01-01

The shaft seal system for the Waste Isolation Pilot Plant (WIPP) must provide a barrier to the migration of fluids within the shafts to prevent the release of contaminants to the accessible environment. To investigate the performance of the shaft seal system, a set of fluid flow performance models was developed based upon the physical characteristics of the WIPP shaft seal system and the surrounding geologic media. This paper describes the results of a numerical model used to investigate the long-term potential for brine migration through the shaft seal system. Modeling results demonstrate that the WIPP shaft seal system will effectively limit brine migration within the repository shafts

Collaborative study for establishment of the European Pharmacopoeia BRP batch 1 for diphtheria toxin.

Science.gov (United States)

Sesardic, D; Prior, C; Daas, A; Buchheit, K H

2003-07-01

A stable liquid candidate Biological Reference Preparation (BRP) for diphtheria toxin was prepared in peptone buffer (nominal content of diphtheria toxin: 1 Lf/ml, 0.4 micro g/ml), filled in ampoules (filling volume: 1 ml) and characterised in a collaborative study. The toxin is to be used in the test "Absence of toxin and irreversibility of toxoid" as described in the current European Pharmacopoeia (Ph. Eur.) monograph Diphtheria Vaccine (Adsorbed) (2002:0443). Eleven laboratories assessed the specific activity of the preparation by in vivo and in vitro assays. The material is assumed to have satisfactory stability with a calculated predicted loss of activity of LD( 50)/ml (lethal challenge) and >75 000 Lr/Lf (intradermal challenge). The candidate BRP was successfully used in nine laboratories and confirmed suitable for use in the Vero cell test for "Absence of toxin and irreversibility of toxoid" as described in the Ph. Eur. monograph 2002:0443; i.e., concentrations of 5 x 10( -5) Lf/ml and below caused cytotoxic effects in the Vero cell test. Due to its liquid nature, the stability of the material will be monitored at regular intervals and preparation of a stable freeze-dried formulation will be considered for long-term use. Additional studies will be performed to confirm suitability of this BRP for other applications. The candidate BRP was adopted as the Ph. Eur. reference material for Diphtheria Toxin Batch 1 by the Ph. Eur. Commission at its session in March 2003.

The Druggable Pocketome of Corynebacterium diphtheriae: A New Approach for in silico Putative Druggable Targets

Science.gov (United States)

Hassan, Syed S.; Jamal, Syed B.; Radusky, Leandro G.; Tiwari, Sandeep; Ullah, Asad; Ali, Javed; Behramand; de Carvalho, Paulo V. S. D.; Shams, Rida; Khan, Sabir; Figueiredo, Henrique C. P.; Barh, Debmalya; Ghosh, Preetam; Silva, Artur; Baumbach, Jan; Röttger, Richard; Turjanski, Adrián G.; Azevedo, Vasco A. C.

2018-01-01

Diphtheria is an acute and highly infectious disease, previously regarded as endemic in nature but vaccine-preventable, is caused by Corynebacterium diphtheriae (Cd). In this work, we used an in silico approach along the 13 complete genome sequences of C. diphtheriae followed by a computational assessment of structural information of the binding sites to characterize the “pocketome druggability.” To this end, we first computed the “modelome” (3D structures of a complete genome) of a randomly selected reference strain Cd NCTC13129; that had 13,763 open reading frames (ORFs) and resulted in 1,253 (∼9%) structure models. The amino acid sequences of these modeled structures were compared with the remaining 12 genomes and consequently, 438 conserved protein sequences were obtained. The RCSB-PDB database was consulted to check the template structures for these conserved proteins and as a result, 401 adequate 3D models were obtained. We subsequently predicted the protein pockets for the obtained set of models and kept only the conserved pockets that had highly druggable (HD) values (137 across all strains). Later, an off-target host homology analyses was performed considering the human proteome using NCBI database. Furthermore, the gene essentiality analysis was carried out that gave a final set of 10-conserved targets possessing highly druggable protein pockets. To check the target identification robustness of the pipeline used in this work, we crosschecked the final target list with another in-house target identification approach for C. diphtheriae thereby obtaining three common targets, these were; hisE-phosphoribosyl-ATP pyrophosphatase, glpX-fructose 1,6-bisphosphatase II, and rpsH-30S ribosomal protein S8. Our predicted results suggest that the in silico approach used could potentially aid in experimental polypharmacological target determination in C. diphtheriae and other pathogens, thereby, might complement the existing and new drug-discovery pipelines

Cyclic Di-GMP Binding by an Assembly ATPase (PilB2) and Control of Type IV Pilin Polymerization in the Gram-Positive Pathogen Clostridium perfringens.

Science.gov (United States)

Hendrick, William A; Orr, Mona W; Murray, Samantha R; Lee, Vincent T; Melville, Stephen B

2017-05-15

The Gram-positive pathogen Clostridium perfringens possesses type IV pili (TFP), which are extracellular fibers that are polymerized from a pool of pilin monomers in the cytoplasmic membrane. Two proteins that are essential for pilus functions are an assembly ATPase (PilB) and an inner membrane core protein (PilC). Two homologues each of PilB and PilC are present in C. perfringens , called PilB1/PilB2 and PilC1/PilC2, respectively, along with four pilin proteins, PilA1 to PilA4. The gene encoding PilA2, which is considered the major pilin based on previous studies, is immediately downstream of the pilB2 and pilC2 genes. Purified PilB2 had ATPase activity, bound zinc, formed hexamers even in the absence of ATP, and bound the second messenger molecule cyclic di-GMP (c-di-GMP). Circular dichroism spectroscopy of purified PilC2 indicated that it retained its predicted degree of alpha-helical secondary structure. Even though no direct interactions between PilB2 and PilC2 could be detected in vivo or in vitro even in the presence of c-di-GMP, high levels of expression of a diguanylate cyclase from C. perfringens (CPE1788) stimulated polymerization of PilA2 in a PilB2- and PilC2-dependent manner. These results suggest that PilB2 activity is controlled by c-di-GMP levels in vivo but that PilB2-PilC2 interactions are either transitory or of low affinity, in contrast to results reported previously from in vivo studies of the PilB1/PilC1 pair in which PilC1 was needed for polar localization of PilB1. This is the first biochemical characterization of a c-di-GMP-dependent assembly ATPase from a Gram-positive bacterium. IMPORTANCE Type IV pili (TFP) are protein fibers involved in important bacterial functions, including motility, adherence to surfaces and host cells, and natural transformation. All clostridia whose genomes have been sequenced show evidence of the presence of TFP. The genetically tractable species Clostridium perfringens was used to study proteins involved in

Procedure for determining the optimum rate of increasing shaft depth

Energy Technology Data Exchange (ETDEWEB)

Durov, E.M.

1983-03-01

Presented is an economic analysis of increasing shaft depth during mine modernization. Investigations carried out by the Yuzhgiproshakht Institute are analyzed. The investigations are aimed at determining the optimum shaft sinking rate (the rate which reduces investment to the minimum). The following factors are considered: coal output of a mine (0.9, 1.2, 1.5 and 1.8 Mt/year), depth at which the new mining level is situated (600, 800, 1200, 1400 and 1600 m), four schemes of increasing depth of 2 central shafts (rock hoisting to ground surface, rock hoisting to the existing level, rock haulage to the developed level, rock haulage to the level being developed using a large diameter borehole drilled from the new level to the shaft bottom and enlarged from shaft bottom to the new level), shaft sinking rate (10, 20, 30, 40, 50 and 60 m/month), range of increasing shaft depth (the difference between depth of the shaft before and after increasing its depth by 100, 200, 300 and 400 m). Comparative evaluations show that the optimum shaft sinking rate depends on the scheme for rock hoisting (one of 4 analyzed), range of increasing shaft depth and gas content in coal seams. The optimum shaft sinking rate ranges from 20 to 40 m/month in coal mines with low methane content and from 20 to 30 m/month in gassy coal mines. The planned coal output of a mine does not influence the optimum shaft sinking rate.

Exploratory Shaft Seismic Design Basis Working Group report

International Nuclear Information System (INIS)

Subramanian, C.V.; King, J.L.; Perkins, D.M.; Mudd, R.W.; Richardson, A.M.; Calovini, J.C.; Van Eeckhout, E.; Emerson, D.O.

1990-08-01

This report was prepared for the Yucca Mountain Project (YMP), which is managed by the US Department of Energy. The participants in the YMP are investigating the suitability of a site at Yucca Mountain, Nevada, for construction of a repository for high-level radioactive waste. An exploratory shaft facility (ESF) will be constructed to permit site characterization. The major components of the ESF are two shafts that will be used to provide access to the underground test areas for men, utilities, and ventilation. If a repository is constructed at the site, the exploratory shafts will be converted for use as intake ventilation shafts. In the context of both underground nuclear explosions (conducted at the nearby Nevada Test Site) and earthquakes, the report contains discussions of faulting potential at the site, control motions at depth, material properties of the different rock layers relevant to seismic design, the strain tensor for each of the waveforms along the shaft liners, and the method for combining the different strain components along the shaft liners. The report also describes analytic methods, assumptions used to ensure conservatism, and uncertainties in the data. The analyses show that none of the shafts' structures, systems, or components are important to public radiological safety; therefore, the shafts need only be designed to ensure worker safety, and the report recommends seismic design parameters appropriate for this purpose. 31 refs., 5 figs., 6 tabs

In vitro pyrogenicity of the diphtheria, tetanus and acellular pertussis components of a trivalent vaccine.

Science.gov (United States)

Carlin, Gunnar; Viitanen, Eila

2005-05-25

We have earlier found that a trivalent vaccine, containing antigenic components from both Gram-positive and Gram-negative bacteria, induced secretion of the endogenous pyrogen interleukin 6 (IL-6) when added to fresh human blood in vitro. The results of the present study showed that the IL-6 secretion was induced by toxoids derived from the Gram-positive bacterium Corynebacterium diphtheriae. However, fresh whole blood from different donors reacted differently to the stimulation. The blood from some donors induced secretion of large concentrations of IL-6, while the blood from other donors induced essentially no IL-6 secretion as a response to stimulation with diphtheria toxoid or a mixture of diphtheria and tetanus toxoids. Repeated testing over several years using blood from the same donor confirmed a donor-dependency of the reaction. This donor-dependency was only found for the toxoid, since blood from all donors reacted with approximately similar IL-6 production to stimulation by endotoxin from the Gram-negative bacterium Escherichia coli, known to be mediated via the toll-like receptor (TLR) 4. Also, no donor-dependecy was found to highly purified lipoteichoic acid from the Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, known to be mediated via TLR-2 and TLR-6. The receptors involved in stimulation by diphtheria toxoid are not known, but may differ from those used by endotoxin and lipoteichoic acid.

Determination of low tetanus or diphtheria antitoxin titers in sera by a toxin neutralization assay and a modified toxin-binding inhibition test

Directory of Open Access Journals (Sweden)

M.H. Sonobe

2007-01-01

Full Text Available A method for the screening of tetanus and diphtheria antibodies in serum using anatoxin (inactivated toxin instead of toxin was developed as an alternative to the in vivo toxin neutralization assay based on the toxin-binding inhibition test (TOBI test. In this study, the serum titers (values between 1.0 and 19.5 IU measured by a modified TOBI test (Modi-TOBI test and toxin neutralization assays were correlated (P < 0.0001. Titers of tetanus or diphtheria antibodies were evaluated in serum samples from guinea pigs immunized with tetanus toxoid, diphtheria-tetanus or triple vaccine. For the Modi-TOBI test, after blocking the microtiter plates, standard tetanus or diphtheria antitoxin and different concentrations of guinea pig sera were incubated with the respective anatoxin. Twelve hours later, these samples were transferred to a plate previously coated with tetanus or diphtheria antitoxin to bind the remaining anatoxin. The anatoxin was then detected using a peroxidase-labeled tetanus or diphtheria antitoxin. Serum titers were calculated using a linear regression plot of the results for the corresponding standard antitoxin. For the toxin neutralization assay, L+/10/50 doses of either toxin combined with different concentrations of serum samples were inoculated into mice for anti-tetanus detection, or in guinea pigs for anti-diphtheria detection. Both assays were suitable for determining wide ranges of antitoxin levels. The linear regression plots showed high correlation coefficients for tetanus (r² = 0.95, P < 0.0001 and for diphtheria (r² = 0.93, P < 0.0001 between the in vitro and the in vivo assays. The standardized method is appropriate for evaluating titers of neutralizing antibodies, thus permitting the in vitro control of serum antitoxin levels.

Is diphtheria-tetanus-pertussis (DTP) associated with increased female mortality?

DEFF Research Database (Denmark)

Aaby, Peter; Ravn, Henrik; Fisker, Ane B

2016-01-01

BACKGROUND: Ten years ago, we formulated two hypotheses about whole-cell diphtheria-tetanus-pertussis (DTP) vaccination: first, when given after BCG, DTP increases mortality in girls and, second, following DTP there is an increase in the female/male mortality rate ratio (MRR). A recent review...

Dynamic changes of horse serum T-globulin immunization with snake venoms, tetanus and diphtheria toxoids.

Science.gov (United States)

Lee, H F; Lee, J D; Lee, Y C

1979-12-01

In course of immunizing horses with snake venoms, tetanus and diphtheria toxoids, a new serum component, T-globulin, was formed and migrated between the beta- and gamma-globulins. The T-globulin content was parallel with the antibody titre after the middle course of immunization. There were many components in snake antivenin and T-globulin was composed of most of those components. The components of diphtheria T-globulin were the same as those of crude antitoxin and tetanus T-globulin except one precipitin.

Storage shaft definitive closure plug and method

International Nuclear Information System (INIS)

Dardaine, M.

1992-01-01

A definitive closure plug system for radioactive waste storage at any deepness, is presented. The inherent weight of the closure materials is used to set in the plug: these materials display an inclined sliding surface in such a way that when the closure material rests on a stable surface of the shaft storage materials, the relative sliding of the different materials tends to spread them towards the shaft internal wall so as to completely occlude the shaft

Long-Term Protection against Diphtheria in the Netherlands after 50 Years of Vaccination: Results from a Seroepidemiological Study.

Science.gov (United States)

Swart, E M; van Gageldonk, P G M; de Melker, H E; van der Klis, F R; Berbers, G A M; Mollema, L

2016-01-01

To evaluate the National Immunisation Programme (NIP) a population-based cross-sectional seroepidemiological study was performed in the Netherlands. We assessed diphtheria antitoxin levels in the general Dutch population and in low vaccination coverage (LVC) areas where a relatively high proportion of orthodox Protestants live who decline vaccination based on religious grounds. Results were compared with a nationwide seroepidemiological study performed 11 years earlier. In 2006/2007 a national serum bank was established. Blood samples were tested for diphtheria antitoxin IgG concentrations using a multiplex immunoassay for 6383 participants from the national sample (NS) and 1518 participants from LVC municipalities. A cut-off above 0.01 international units per ml (IU/ml) was used as minimum protective level. In the NS 91% of the population had antibody levels above 0.01 IU/ml compared to 88% in the 1995/1996 serosurvey (pdiphtheria vaccination in the NIP and 46% (vs. 37% in the 1995/1996 serosurvey, p = 0.11) of orthodox Protestants living in LVC areas had antibody levels above 0.01 IU/ml. Linear regression analysis among fully immunized individuals (six vaccinations) without evidence of revaccination indicated a continuous decline in antibodies in both serosurveys, but geometric mean antibodies remained well above 0.01 IU/ml in all age groups. The NIP provides long-term protection against diphtheria, although antibody levels decline after vaccination. As a result of natural waning immunity, a substantial proportion of individuals born before introduction of diphtheria vaccination in the NIP lack adequate levels of diphtheria antibodies. Susceptibility due to lack of vaccination is highest among strictly orthodox Protestants. The potential risk of spread of diphtheria within the geographically clustered orthodox Protestant community after introduction in the Netherlands has not disappeared, despite national long-term high vaccination coverage.

Salt Repository Project shaft design guide: Revision 0

International Nuclear Information System (INIS)

1987-12-01

The Salt Repository Project (SRP) Shaft Design Guide (SDG) and the accompanying SRP Input to Seismic Design define the basic approach for developing appropriate shaft designs for a high-level nuclear waste repository in salt at a proposed site in Deaf Smith County, Texas. The SDG is based on current mining industry standards and practices enhanced to meet the special needs of an underground nuclear waste repository. It provides a common approach for design of both the exploratory and repository shafts. The SDG defines shaft lining and material concepts and presents methods for calculating the loads and displacements that will be imposed on lining structures. It also presents the methodology and formulae for sizing lining components. The SDG directs the shaft designer to sources of geoscience and seismic design data for the Deaf Smith County, Texas repository site. In addition, the SDG describes methods for confirming shaft lining design by means of computer analysis, and it discusses performance monitoring needs that must be considered in the design. 113 refs., 18 figs., 14 tabs

Semicarbazone EGA Inhibits Uptake of Diphtheria Toxin into Human Cells and Protects Cells from Intoxication

Directory of Open Access Journals (Sweden)

Leonie Schnell

2016-07-01

Full Text Available Diphtheria toxin is a single-chain protein toxin that invades human cells by receptor-mediated endocytosis. In acidic endosomes, its translocation domain inserts into endosomal membranes and facilitates the transport of the catalytic domain (DTA from endosomal lumen into the host cell cytosol. Here, DTA ADP-ribosylates elongation factor 2 inhibits protein synthesis and leads to cell death. The compound 4-bromobenzaldehyde N-(2,6-dimethylphenylsemicarbazone (EGA has been previously shown to protect cells from various bacterial protein toxins which deliver their enzymatic subunits from acidic endosomes to the cytosol, including Bacillus anthracis lethal toxin and the binary clostridial actin ADP-ribosylating toxins C2, iota and Clostridium difficile binary toxin (CDT. Here, we demonstrate that EGA also protects human cells from diphtheria toxin by inhibiting the pH-dependent translocation of DTA across cell membranes. The results suggest that EGA might serve for treatment and/or prevention of the severe disease diphtheria.

Gearbox Reliability Collaborative High-Speed Shaft Calibration

Energy Technology Data Exchange (ETDEWEB)

Keller, J.; McNiff, B.

2014-09-01

Instrumentation has been added to the high-speed shaft, pinion, and tapered roller bearing pair of the Gearbox Reliability Collaborative gearbox to measure loads and temperatures. The new shaft bending moment and torque instrumentation was calibrated and the purpose of this document is to describe this calibration process and results, such that the raw shaft bending and torque signals can be converted to the proper engineering units and coordinate system reference for comparison to design loads and simulation model predictions.

Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide

Energy Technology Data Exchange (ETDEWEB)

Liu, Bing; Garnett, James A.; Lee, Wei-chao; Lin, Jing; Salgado, Paula; Taylor, Jonathan; Xu, Yingqi; Lambert, Sebastian; Cota, Ernesto [Centre for Structural Biology and Division of Molecular Biosciences, Imperial College London, South Kensington, London SW7 2AZ (United Kingdom); Matthews, Steve, E-mail: s.j.matthews@imperial.ac.uk [Centre for Structural Biology and Division of Molecular Biosciences, Imperial College London, South Kensington, London SW7 2AZ (United Kingdom)

2012-05-04

Highlights: Black-Right-Pointing-Pointer The benefits of D{sub 2}O in screening for crystallisation was explored. Black-Right-Pointing-Pointer The crystal structures of the SefD pilin in both H{sub 2}O and D{sub 2}O reveal differences. Black-Right-Pointing-Pointer Crystallisation improvements are explained by altered interactions in D{sub 2}O crystals. Black-Right-Pointing-Pointer D{sub 2}O is useful additive in sparse-matrix screening for crystallisation. -- Abstract: The use of heavy water (D{sub 2}O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D{sub 2}O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.

Increasing shaft depth with rock hoisting to the surface. [USSR

Energy Technology Data Exchange (ETDEWEB)

Durov, E.M.

1982-06-01

Schemes of shaft construction with increasing shaft depth depend on: shaft depth, shaft diameter, types of hoisting systems, schemes of shaft reinforcement. Investigations carried out in underground coal mines in the USSR show that waste rock haulage to the surface by an independent hoisting system is most economical. Installation of this system depends on the existing hoisting scheme. When one of the operating cages or skips can be removed without a negative influence on mine operation the system of rock waste hoisting is used. The hoisting bucket used for rock removal from the shaft bottom moves in the shaft section from which one of the cages or skips has been removed. Examples of using this scheme in Donbass, Kuzbass and other coal basins are given. Economic aspects of waste material hoisting to the surface are analyzed. The system is economical when the remaining hoisting system can accept additional loads after removal of a cage or skip from the shaft. Investigations show that use of a bucket with a capacity from 2.5 to 3.0 m/sup 3/ for waste rock removal from the shaft being modernized and deepened is most economical.

Prevalence of diphtheria and tetanus antibodies among adults in Singapore: a national serological study to identify most susceptible population groups.

Science.gov (United States)

Ang, L W; James, L; Goh, K T

2016-03-01

In view of waning antitoxin titres over time after the last vaccine dose against diphtheria and tetanus, we determined the immunity levels in adults to identify most susceptible groups for protection in Singapore. Our study involved residual sera from 3293 adults aged 18-79 who had participated in a national health survey in 2010. IgG antibody levels were determined using commercial enzyme-linked immunosorbent assay. Overall, 92.0% (95% confidence interval [CI]: 91.1-92.9%) had at least basic protection against diphtheria (antibody levels ≥0.01 IU/ml), while 71.4% (95% CI: 69.8-72.9%) had at least short-term protection against tetanus (antibody levels >0.1 IU/ml). The seroprevalence declined significantly with age for both diseases; the drop was most marked in the 50- to 59-year age group for diphtheria and 60- to 69-year age group for tetanus. There was a significant difference in seroprevalence by residency for diphtheria (92.8% among Singapore citizens versus 87.1% among permanent residents; P = 0.001). The seroprevalence for tetanus was significantly higher among males (83.2%) than females (62.4%) (P < 0.0005). It may be of value to consider additional vaccination efforts to protect older adults at higher risk for exposure against diphtheria and tetanus, particularly those travelling to areas where diphtheria is endemic or epidemic. © The Author 2015. Published by Oxford University Press on behalf of Faculty of Public Health. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Ipsilateral humeral neck and shaft fractures

Directory of Open Access Journals (Sweden)

Zhu Bin

2017-01-01

Full Text Available Background/Aim. Fractures of the proximal humerus or shaft are common, however, ipsilateral neck and shaft humerus fracture is a rare phenomenon. This combination injury is challenging for orthopaedic surgeons because of its complex treatment options at present. The purpose of this study was to review a series of ipsilateral humeral neck and shaft fractures to study the fracture pattern, complications and treatment outcomes of each treatment options used. Methods. A total of six patients (four female and two male with the average age of 42.8 years (range: 36–49 years was collected and reviewed retrospectively. Two of them were treated with double plates and four with antegrade intramedullary nail. According to the Neer’s classification, all proximal fractures were two-part surgical neck fractures. All humeral shaft fractures were located at the middle of one third. Five fractures were simple transverse (A3, one fragmented wedge fracture (B3. One patient had associated radial nerve palsy. Results. All surgical neck fractures except one united uneventfully in the average time span of 8.7 weeks. Four humeral shaft fractures healed in near anatomic alignment. The remaining two patients had the nonunion with no radiological signs of fracture healing. The average University of California, Los Angeles End-Results (UCLA score was 23.1. On the contrary, the average American Shoulder and Elbow Surgeon's (ASES score was 73.3. The patients treated with antegrade intramedullary nails presented 70.5 points. The ASES scores were 79 in the double plates group. Conclusions. Ipsilateral humeral shaft and neck fracture is extremely rare. Both antegrade intramedullar nailing and double plates result in healing of fractures. However the risk of complication is lower in the double plating group.

A cross-reacting material CRM197 conjugate vaccine induces diphtheria toxin neutralizing antibody response in children and adolescents infected or not with HIV.

Science.gov (United States)

Silva, Giselle P; Santos, Rafaela S; Pereira-Manfro, Wânia F; Ferreira, Bianca; Barreto, Daniella M; Frota, Ana Cristina C; Hofer, Cristina B; Milagres, Lucimar G

2017-07-05

Anti-diphtheria antibody levels decrease with aging, and frequent booster vaccinations are required to maintain herd immunity. We analyzed the diphtheria toxin neutralizing antibody (DT-Nab) response induced by a conjugate vaccine (meningococcal C polysaccharide-CRM 197 ) in HIV-vertically infected (HI) children and adolescents and healthy controls (HC) with matched age. We report the association of DT-Nab with the bactericidal antibodies to serogroup C meningococcus (MenC). Before vaccination, 21 HI patients (50%) had no protection against diphtheria (≤0.01IU/ml of antibody) and only 8 (19%) showed complete protection (≥0.1IU/ml). About half of the HC (56%) had complete protection before immunization and 6 subjects (12%) had no protection against diphtheria. After one and two vaccine injections, 96% of HC and 64% of HI vaccinees, respectively, showed full protection against diphtheria. These data indicate that CRM 197 was able to induce primary and/or booster response in both groups of individuals. Copyright © 2017 Elsevier Ltd. All rights reserved.

Construction features of the Exploratory Shaft at Yucca Mountain

International Nuclear Information System (INIS)

Adair, G.W.; Fiore, J.N.

1984-01-01

The Exploratory Shaft (ES) at Yucca Mountain is planned to be constructed during 1985 and 1986 as part of the detailed site characterization for one of three sites which may be selected as candidates for location of a high-level radioactive waste repository. Conventional mining methods will be used for the shaft sinking phase of the ES project. The ES will be comprised of surface support facilities, a 1,480-foot-deep circular shaft lined with concrete to a finished inside diameter of 12 feet, lateral excavations and test installations extending up to 200 feet from the shaft, and long lateral borings extending up to 2,300 feet from the shaft. The estimated time for sinking the shaft to a total depth of about 1,480 feet and completing the lateral excavations and borings is about two years. The major underground development planned for the primary test level at a depth of 1,200 feet consists of the equivalent of 1,150 feet of 15- by 15-foot drift. The total volume of rock to be removed from the shaft proper and the lateral excavations totals about 1/2 million cubic feet. Construction equipment for the shaft and underground excavation phases consists of conventional mine hoisting equipment, shot hole and rock bolt drilling jumbos, mucking machines, and hauling machines. The desire to maintain relatively uniform and even walls in selected shaft and drift intervals will require that controlled blasting techniques be employed. Such techniques generally classified as ''smooth blasting'' are commonly used for excavation in the construction industry

System design for shaft safety and productivity

Energy Technology Data Exchange (ETDEWEB)

Owen, D.; Parsons, R.; Ward, R.

1988-03-01

The aim of this paper is to describe the process of designing a system to improve safety and productivity in shafts. The objectives and constraints for the design were set out in official reports following a shaft accident at Markham Colliery in 1973. The problems to be solved were: to enable the shaftsmen to transfer the existing statutory code of signals efficiently from, or on top of, a conveyance anywhere in the shaft to the winding engineman and banksman at the surface: to detect the existence of slack rope or to detect that conditions have arisen that slack rope could be created and transmit this information to where action can be taken; and to allow conversations between winding engineman, banksman and shaftsman making allowances for the high level of acoustic noise in shafts. The approach adopted for slack rope monitoring was to monitor the tension in the cage suspension gear, thus measuring a first order effect. The three problems have a common element: information must be transferred through the shaft. This particular problem was solved with guided radio, using the winding rope as the transmission medium. The radio signal is coupled into the winding rope by means of fixed toroid encircling it at the cage and fixed magnetic antennas at the surface. The design of a digital transmission system for signalling and tension data is discussed. The 'top down' modular approach used in the design enabled full advantage to be taken of the opportunities for building a more reliable, safer and flexible system presented by technologies new to the shaft environment. The resultant system, the Safecom Shaft Signalling Communication and Winder Safety Monitoring System type S100, is in regular use at over 20 installations. 3 refs., 4 figs., 1 tab.

Exploratory Shaft Facility design basis study report

International Nuclear Information System (INIS)

Langstaff, A.L.

1987-01-01

The Design Basis Study is a scoping/sizing study that evaluated the items concerning the Exploratory Shaft Facility Design including design basis values for water and methane inflow; flexibility of the design to support potential changes in program direction; cost and schedule impacts that could result if the design were changed to comply with gassy mine regulations; and cost, schedule, advantages and disadvantages of a larger second shaft. Recommendations are proposed concerning water and methane inflow values, facility layout, second shaft size, ventilation, and gassy mine requirements. 75 refs., 3 figs., 7 tabs

Autoradiographic assay of mutants resistant to diphtheria toxin in mammalian cells in vitro

International Nuclear Information System (INIS)

Ronen, A.; Gingerich, J.D.; Duncan, A.M.V.; Heddle, J.A.

1984-01-01

Diptheria toxin kills mammalian cells by ribosylating elongation factor 2, a protein factor necessary for protein synthesis. The frequency of cells able to form colonies in the presence of the toxin can be used as an assay for mutation to diphtheria toxin resistance. Resistance to diphtheria toxin can also be detected autoradiographically in cells exposed to [ 3 H]leucine after treatment with the toxin. In cultures of Chinese hamster ovary cells, the frequency of such resistant cells is increased by exposure of the cells to γ-rays, ultraviolet light, ethylnitrosourea, mitomycin c, ethidium bromide, and 5-bromo-2'-deoxyuridine in a dose- and time-dependent manner. The resistant cells form discrete microcolonies if they are allowed to divide several times before intoxication which indicates that they are genuine mutants. The assay is potentially adaptable to any cell population that can be intoxicated with diphtheria toxin and labeled with [ 3 H]leucine, whether or not the cells can form colonies. It may be useful, therefore, for measuring mutation rates in slowly growing or nondividing cell populations such as breast, brain, and liver, as well as in cells that do divide but cannot be readily cloned, such as the colonic epithelium. 23 references, 6 figures

Ipsilateral femoral neck and shaft fractures: An overlooked association

International Nuclear Information System (INIS)

Daffner, R.H.; Riemer, B.L.; Butterfield, S.L.

1991-01-01

A total of 304 patients with injuries to the femoral shaft and ipsilateral hip presented between 1984 and 1990. Some 253 of them suffered fractures of the femoral shaft and dislocated hips or fractures of the acetabulum, and 51 of these sustained fractures of the femoral shaft and neck or trochanteric region. All of the trochanteric injuries were demonstrated on the initial radiographs. However, in 11 of the patients with combined femoral shaft and neck fractures, the diagnosis was delayed by as much as 4 weeks. This delay related to the fact that these fractures tended not to separate in the initial evaluation period and that there was external rotation of the proximal femoral fragment due to the femoral shaft fracture. (orig./GDG)

Ipsilateral femoral neck and shaft fractures: An overlooked association

Energy Technology Data Exchange (ETDEWEB)

Daffner, R.H. (Dept. of Diagnostic Radiology, Allegheny General Hospital, Pittsburgh, PA (USA) Medical Coll. of Pennsylvania, Pittsburgh, PA (USA)); Riemer, B.L.; Butterfield, S.L. (Dept. of Orthopedic Surgery, Allegheny General Hospital, Pittsburgh, PA (USA) Medical Coll. of Pennsylvania, Pittsburgh, PA (USA))

1991-05-01

A total of 304 patients with injuries to the femoral shaft and ipsilateral hip presented between 1984 and 1990. Some 253 of them suffered fractures of the femoral shaft and dislocated hips or fractures of the acetabulum, and 51 of these sustained fractures of the femoral shaft and neck or trochanteric region. All of the trochanteric injuries were demonstrated on the initial radiographs. However, in 11 of the patients with combined femoral shaft and neck fractures, the diagnosis was delayed by as much as 4 weeks. This delay related to the fact that these fractures tended not to separate in the initial evaluation period and that there was external rotation of the proximal femoral fragment due to the femoral shaft fracture. (orig./GDG).

Diphtheria toxin-induced channels in Vero cells selective for monovalent cations

International Nuclear Information System (INIS)

Sandvig, K.; Olsnes, S.

1988-01-01

Ion fluxes associated with translocation of diphtheria toxin across the surface membrane of Vero cells were studied. When cells with surface-bound toxin were exposed to low pH to induce toxin entry, the cells became permeable to Na+, K+, H+, choline+, and glucosamine+. There was no increased permeability to Cl-, SO4(-2), glucose, or sucrose, whereas the uptake of 45 Ca2+ was slightly increased. The influx of Ca2+, which appears to be different from that of monovalent cations, was reduced by several inhibitors of anion transport and by verapamil, Mn2+, Co2+, and Ca2+, but not by Mg2+. The toxin-induced fluxes of N+, K+, and protons were inhibited by Cd2+. Cd2+ also protected the cells against intoxication by diphtheria toxin, suggesting that the open cation-selective channel is required for toxin translocation. The involvement of the toxin receptor is discussed

Spiral groove seal. [for hydraulic rotating shaft

Science.gov (United States)

Ludwig, L. P. (Inventor)

1973-01-01

Mating flat surfaces inhibit leakage of a fluid around a stationary shaft. A spiral groove pattern produces a pumping action toward the fluid when the shaft rotates which prevents leakage while a generated hydraulic lifting force separates the mating surfaces to minimize wear.

Dynamic analysis of cross shaft type universal joint with clearance

International Nuclear Information System (INIS)

Lu, Jian Wei; Wang, Gong Cheng; Chen, Hao; Vakakis, Alexander F.; Bergman, Lawrence A.

2013-01-01

Cross shaft type universal joint is widely used in ground vehicles to transfer torque between two intersecting axes, and its transmission feature can make a great contribution to NVH performance of the vehicle. We looked at the assembling clearance at cross shaft neck, and presented a dynamic model of cross shaft type universal joint with clearance at cross shaft neck. Two-state model is applied to describe the contact force between the cross shaft and driving joint fork based on Hertz theorem, and lumped mass method is applied to build up the dynamic model of the universal joint. Based on this model, numerical analysis is carried out to discuss the transmission feature of the universal joint with clearance at cross shaft neck, and the influence of clearance on the dynamic behavior of the system is evaluated with numerical results based on time history, power spectrum, and phase portrait. The method and conclusions presented are helpful to improvement of the transmission feature of cross shaft type universal joint.

Shaft siting decision report: Final report

International Nuclear Information System (INIS)

1985-11-01

The purpose of this study is to identify and establish relative guidelines to be used for siting of repository shafts. Weights were determined for the significant factors which impact the selection of shaft locations for a nuclear waste repository in salt. The study identified a total of 45 factors. A panel of experienced mining people utilized the Kepner-Tregoe (K-T) Decision Analysis Process to perform a structured evaluation of each significant shaft siting factor. The evaluation determined that 22 of the factors were absolute constraints and that the other 23 factors were desirable characteristics. The group established the relative weights for each of the 23 desirable characteristics by using a paired comparison method. 49 refs., 2 figs., 5 tabs

A coupled mechanical/hydrologic model for WIPP shaft seals

International Nuclear Information System (INIS)

Ehgartner, B.

1991-06-01

Effective sealing of the Waste Isolation Pilot Plant (WIPP) shafts will be required to isolate defense-generated transuranic wastes from the accessible environment. Shafts penetrate water-bearing hard rock formations before entering a massive creeping-salt formation (Salado) where the WIPP is located. Short and long-term seals are planned for the shafts. Short-term seals, a composite of concrete and bentonite, will primarily be located in the hard rock formations separating the water-bearing zones from the Salado Formation. These seals will limit water flow to the underlying long-term seals in the Salado. The long-term seals will consist of lengthly segments of initially unsaturated crushed salt. Creep closure of the shaft will consolidate unsaturated crushed salt, thereby reducing its permeability. However, water passing through the upper short-term seals and brine inherent to the salt host rock itself will eventually saturate the crushed salt and consolidation could be inhibited. Before saturating, portions of the crushed salt in the shafts are expected to consolidate to a permeability equivalent to the salt host rock, thereby effectively isolating the waste from the overlying water-bearing formations. A phenomenological model is developed for the coupled mechanical/hydrologic behavior of sealed WIPP shafts. The model couples creep closure of the shaft, crushed salt consolidation, and the associated reduction in permeability with Darcy's law for saturated fluid flow to predict the overall permeability of the shaft seal system with time. 17 refs., 6 figs., 1 tab

Shaft Siting and Configuration for Flexible Operating Mode

International Nuclear Information System (INIS)

Robert Boutin

2001-01-01

The purpose of this document as stated in the ''Technical Work Plan for Subsurface Design Section FY 01 Work Activities'' (CRWMS M and O 2001a, pg. 14) is to review and evaluate the most current concepts for shaft siting and configuration. The locations of the shaft sites will be evaluated in reference to the overall subsurface ventilation layout shown in Figure 1. The scope will include discussions on pad size requirements, shaft construction components such as collars, shaft stations, sumps, ground support and linings, head frames, fan ducting and facility equipping. In addition to these, shaft excavation methodologies and integration with the overall subsurface construction schedule will be described. The Technical Work Plan (TWP), (CRWMS M and O 2001a), for this document has been prepared in accordance with AP-2.21Q, ''Quality Determinations and Planning for Scientific, Engineering and Regulatory Compliance Activities''. This document will be prepared in accordance with AP-3.10Q, ''Analysis and Models''. This document contributes to Site Recommendation (SR). The intended use of this document is to provide an analysis for shaft siting and configuration criteria for subsequent construction. This document identifies preliminary design concepts that should not be used for procurement, fabrication, or construction

Humeral Shaft Fracture: Intramedullary Nailing.

Science.gov (United States)

Konda, Sanjit R; Saleh, Hesham; Fisher, Nina; Egol, Kenneth A

2017-08-01

This video demonstrates the technique of intramedullary nailing for a humeral shaft fracture. The patient is a 30-year-old man who sustained a gunshot wound to his right arm. The patient was indicated for humeral nailing given the comminuted nature of the diaphysis and to allow for minimal skin incisions. Other relative indications include soft-tissue compromise about the arm precluding a large surgical exposure. This video presents a case of a comminuted humeral shaft fracture treated with an intramedullary nail. Anatomic reduction and stable fixation was obtained with this technique. This case demonstrates a soft-tissue sparing technique of humeral shaft fixation using a humeral intramedullary nail. The technique is easy to perform and has significant benefits in minimizing surgical exposure, decreasing operative time, and decreasing blood loss. In the correct clinical setting, humeral nailing provides an expeditious form of fixation that restores length, alignment, and rotation of the fracture humeral diaphysis.

Large shaft development test plan

International Nuclear Information System (INIS)

Krug, A.D.

1984-03-01

This test plan proposes the conduct of a large shaft development test at the Hanford site in support of the repository development program. The purpose and objective of the test plan is to obtain the information necessary to establish feasibility and to predict the performance of the drilling system used to drill large diameter shafts. The test plan is based upon drilling a 20 ft diameter shaft to a depth of 1,000 feet. The test plan specifies series of tests to evaluate the performance of the downhole assembly, the performance of the rig, and the ability of the system to cope with geologic hazards. The quality of the hole produced will also be determined. This test plan is considered to be preliminary in that it was prepared as input for the decision to determine if development testing is required in this area. Should the decision be made to proceed with development testing, this test plan shall be updated and revised. 6 refs., 2 figs., 3 tabs

Exploratory shaft liner corrosion estimate

International Nuclear Information System (INIS)

Duncan, D.R.

1985-10-01

An estimate of expected corrosion degradation during the 100-year design life of the Exploratory Shaft (ES) is presented. The basis for the estimate is a brief literature survey of corrosion data, in addition to data taken by the Basalt Waste Isolation Project. The scope of the study is expected corrosion environment of the ES, the corrosion modes of general corrosion, pitting and crevice corrosion, dissimilar metal corrosion, and environmentally assisted cracking. The expected internal and external environment of the shaft liner is described in detail and estimated effects of each corrosion mode are given. The maximum amount of general corrosion degradation was estimated to be 70 mils at the exterior and 48 mils at the interior, at the shaft bottom. Corrosion at welds or mechanical joints could be significant, dependent on design. After a final determination of corrosion allowance has been established by the project it will be added to the design criteria. 10 refs., 6 figs., 5 tabs

Diphtheria, pertussis, and tetanus: evidence-based management of pediatric patients in the emergency department

Science.gov (United States)

Zibners, Lara

2017-02-01

Diphtheria, pertussis, and tetanus are potentially deadly bacterial infections that are largely preventable through vaccination, though they remain in the population. This issue reviews the epidemiology, pathophysiology, diagnosis, and current recommended emergency management of these conditions. Disease-specific medications, as well as treatment of the secondary complications, are examined in light of the best current evidence. Resources include obtaining diphtheria antitoxin from the United States Centers for Disease Control and Prevention and best-practice recommendations with regard to testing, involvement of government health agencies, isolation of the patient, and identification and treatment of close contacts. Most importantly, issues regarding vaccination and prevention are highlighted.

Rotating Shaft Tilt Angle Measurement Using an Inclinometer

OpenAIRE

Luo Jun; Wang Zhiqian; Shen Chengwu; Wen Zhuoman; Liu Shaojin; Cai Sheng; Li Jianrong

2015-01-01

This paper describes a novel measurement method to accurately measure the rotating shaft tilt angle of rotating machine for alignment or compensation using a dual-axis inclinometer. A model of the rotating shaft tilt angle measurement is established using a dual-axis inclinometer based on the designed mechanical structure, and the calculation equation between the rotating shaft tilt angle and the inclinometer axes outputs is derived under the condition that the inclinometer axes are perpendic...

30 CFR 77.1911 - Ventilation of slopes and shafts.

Science.gov (United States)

2010-07-01

... SAFETY AND HEALTH MANDATORY SAFETY STANDARDS, SURFACE COAL MINES AND SURFACE WORK AREAS OF UNDERGROUND COAL MINES Slope and Shaft Sinking § 77.1911 Ventilation of slopes and shafts. (a) All slopes and... connected to the slope or shaft opening with fireproof air ducts; (3) Designed to permit the reversal of the...

An integrative in-silico approach for therapeutic target identification in the human pathogen Corynebacterium diphtheriae.

Directory of Open Access Journals (Sweden)

Syed Babar Jamal

Full Text Available Corynebacterium diphtheriae (Cd is a Gram-positive human pathogen responsible for diphtheria infection and once regarded for high mortalities worldwide. The fatality gradually decreased with improved living standards and further alleviated when many immunization programs were introduced. However, numerous drug-resistant strains emerged recently that consequently decreased the efficacy of current therapeutics and vaccines, thereby obliging the scientific community to start investigating new therapeutic targets in pathogenic microorganisms. In this study, our contributions include the prediction of modelome of 13 C. diphtheriae strains, using the MHOLline workflow. A set of 463 conserved proteins were identified by combining the results of pangenomics based core-genome and core-modelome analyses. Further, using subtractive proteomics and modelomics approaches for target identification, a set of 23 proteins was selected as essential for the bacteria. Considering human as a host, eight of these proteins (glpX, nusB, rpsH, hisE, smpB, bioB, DIP1084, and DIP0983 were considered as essential and non-host homologs, and have been subjected to virtual screening using four different compound libraries (extracted from the ZINC database, plant-derived natural compounds and Di-terpenoid Iso-steviol derivatives. The proposed ligand molecules showed favorable interactions, lowered energy values and high complementarity with the predicted targets. Our proposed approach expedites the selection of C. diphtheriae putative proteins for broad-spectrum development of novel drugs and vaccines, owing to the fact that some of these targets have already been identified and validated in other organisms.

Humoral immunity 10 years after booster immunization with an adolescent and adult formulation combined tetanus, diphtheria, and 5-component acellular pertussis vaccine.

Science.gov (United States)

Tomovici, A; Barreto, L; Zickler, P; Meekison, W; Noya, F; Voloshen, T; Lavigne, P

2012-03-30

Persistence of antibodies after a single dose of Tdap vaccine (tetanus, diphtheria, and 5-component acellular pertussis vaccine) was evaluated in a follow-up study of adolescents (N=324) and adults (N=644) who had received Tdap in earlier clinical trials. Outcome measures were seroprotection (tetanus and diphtheria) or seropositivity (pertussis) and geometric mean concentrations. Humoral immune responses to all antigens were robust 1 month after initial immunization, decreased at subsequent measurements, but continued to exceed pre-immunization levels 1, 3, 5, and 10 years later. Protective levels of diphtheria and tetanus antitoxin persisted in 99.3% of adolescents 10 years after a booster dose of Tdap. Seropositivity to 1 or more pertussis antigens also persisted in most adolescents for 10 years. Although tetanus antitoxin responses were similar in adults to those observed in adolescents, diphtheria antitoxin titers were lower, reflecting the fact that a smaller proportion of adults had received diphtheria toxoid in the previous 10 years compared to adolescents. These data will contribute to the selection of the optimal interval for repeat doses of Tdap. Copyright © 2012 Elsevier Ltd. All rights reserved.

Spiral groove seal. [for rotating shaft

Science.gov (United States)

Ludwig, L. P.; Strom, T. N. (Inventor)

1974-01-01

Mating flat surfaces inhibit leakage of a fluid around a stationary shaft. A spiral groove produces a pumping action toward the fluid when the shaft rotates. This prevents leakage while a generated hydraulic lifting force separates the mating surfaces to minimize wear. Provision is made for placing these spiral grooves in communication with the fluid to accelerate the generation of the hydraulic lifting force.

Documentation and verification of the SHAFT code

International Nuclear Information System (INIS)

St John, C.M.

1991-12-01

The SHAFT code incorporates equations to compute stresses in a shaft liner when the rock through which a shaft passes is subject to known three-dimensional states of stress or strain. The deformation modes considered are hoop deformation, axial deformation, and shear on a plane normal to the shaft axis. Interaction between the liner and the soil and rock is considered, and it is assumed that the liner is in place before loading is applied. This code is intended to be used interactively but creates a permanent record complete with necessary quality assurance information. The code has been carefully verified for the case of generalized plane strain, in which an arbitrary axial strain can be defined. It may also be used for plane stress analysis. Output is given in the form of stresses at selected sample points in the linear and the rock and a simple graphical representation of the distribution of stress through the liner. 12 figs., 13 tabs

Incidence and epidemiology of tibial shaft fractures

DEFF Research Database (Denmark)

Larsen, Peter; Elsøe, Rasmus; Hansen, Sandra Hope

2015-01-01

Introduction: The literature lacks recent population-based epidemiology studies of the incidence, trauma mechanism and fracture classification of tibial shaft fractures. The purpose of this study was to provide up-to-date information on the incidence of tibial shaft fractures in a large....... The mean age at time of fracture was 38.5 (21.2SD) years. The incidence of tibial shaft fracture was 16.9/100,000/year. Males have the highest incidence of 21.5/100,000/year and present with the highest frequency between the age of 10 and 20, whereas women have a frequency of 12.3/100,000/year and have...... frequency of fractures while participating in sports activities and walking. Women present the highest frequency of fractures while walking and during indoor activities. Conclusion: This study shows an incidence of 16.9/100,000/year for tibial shaft fractures. AO-type 42-A1 was the most common fracture type...

Residual torsional properties of composite shafts subjected to impact loadings

International Nuclear Information System (INIS)

Sevkat, Ercan; Tumer, Hikmet

2013-01-01

Highlights: • Impact loading reduces the torsional strength of composite shaft. • Impact energy level determines the severity of torsional strength reduction. • Hybrid composite shafts can be manufactured by mixing two types of filament. • Maximum torque capacity of shafts can be estimated using finite element method. - Abstract: This paper presents an experimental and numerical study to investigate residual torsional properties of composite shafts subjected to impact loadings. E-glass/epoxy, carbon/epoxy and E-glass–carbon/epoxy hybrid composite shafts were manufactured by filament winding method. Composite shafts were impacted at 5, 10, 20 and 40 J energy levels. Force–time and energy–time histories of impact tests were recorded. One composite shaft with no impact, and four composite shafts with impact damage, five in total, were tested under torsion. Torque-twisting angle relations for each test were obtained. Reduction at maximum torque and maximum twisting angle induced by impact loadings were calculated. While 5 J impact did not cause significant reduction at maximum torque and maximum twisting angle, remaining impact loadings caused 34–67% reduction at maximum torque, and 30–61% reduction at maximum twisting angle. Reductions increased with increasing energy levels and varied depending on the material of composite shafts. The 3-D finite element (FE) software, Abaqus, incorporated with an elastic orthotropic model, was then used to simulate the torsion tests. Good agreement between experimental and numerical results was achieved

Eddy Current Sensing of Torque in Rotating Shafts

Science.gov (United States)

Varonis, Orestes J.; Ida, Nathan

2013-12-01

The noncontact torque sensing in machine shafts is addressed based on the stress induced in a press-fitted magnetoelastic sleeve on the shaft and eddy current sensing of the changes of electrical conductivity and magnetic permeability due to the presence of stress. The eddy current probe uses dual drive, dual sensing coils whose purpose is increased sensitivity to torque and decreased sensitivity to variations in distance between probe and shaft (liftoff). A mechanism of keeping the distance constant is also employed. Both the probe and the magnetoelastic sleeve are evaluated for performance using a standard eddy current instrument. An eddy current instrument is also used to drive the coils and analyze the torque data. The method and sensor described are general and adaptable to a variety of applications. The sensor is suitable for static and rotating shafts, is independent of shaft diameter and operational over a large range of torques. The torque sensor uses a differential eddy current measurement resulting in cancellation of common mode effects including temperature and vibrations.

Assessment of shaft safety and management system of controlling engineering information

Energy Technology Data Exchange (ETDEWEB)

Liu Rui-xin; Xu Yan-chun [Yanzhou Mining Group Ltd., Zoucheng (China)

2008-02-15

Evaluating shaft safety and establishing a system for controlling engineering information is very important because more than 90 shafts in thick alluvial areas suddenly have shaft wall fracturing or breaking problems and there are more than a few hundred shafts of similar geologic conditions. Taking shaft control in the Yangzhou Coal Mining Group as an example, an assessment and management system and related software were established. This system includes basic information of the mine, measurement results and analysis, and functions of empirical and theoretical forecasting and finite element analysis, which are confirmed to be very effective for guiding shaft well control engineering in practice. 8 refs., 3 figs., 2 tabs.

Outline and results of study on excavation response of rock mass around shaft in shaft excavation effects project

International Nuclear Information System (INIS)

Sugihara, Kozo; Matsui, Hiroya; Sato, Toshinori

1993-01-01

A shaft, with a diameter of 6 m and a depth of 150 m, has been newly excavated in sedimentary rock and excavation response of rock mass around the shaft has been measured and analyzed. Excavation response has been evaluated based on the results of measurement of rock mass movement, such as displacement and strain, and change of rock property, such as deformability and permeability. This study indicates that rock property has been changed with in about 1 m from the shaft wall, and rock mass movement and property change has been influenced by rock facies, fracture and re-distributed stress. The relation between property change and these factors is remained to be evaluated in future study. (author)

DC Control Effort Minimized for Magnetic-Bearing-Supported Shaft

Science.gov (United States)

Brown, Gerald V.

2001-01-01

A magnetic-bearing-supported shaft may have a number of concentricity and alignment problems. One of these involves the relationship of the position sensors, the centerline of the backup bearings, and the magnetic center of the magnetic bearings. For magnetic bearings with permanent magnet biasing, the average control current for a given control axis that is not bearing the shaft weight will be minimized if the shaft is centered, on average over a revolution, at the magnetic center of the bearings. That position may not yield zero sensor output or center the shaft in the backup bearing clearance. The desired shaft position that gives zero average current can be achieved if a simple additional term is added to the control law. Suppose that the instantaneous control currents from each bearing are available from measurements and can be input into the control computer. If each control current is integrated with a very small rate of accumulation and the result is added to the control output, the shaft will gradually move to a position where the control current averages to zero over many revolutions. This will occur regardless of any offsets of the position sensor inputs. At that position, the average control effort is minimized in comparison to other possible locations of the shaft. Nonlinearities of the magnetic bearing are minimized at that location as well.

A review of drilled shaft sealing for the Basalt Waste Isolation Project

International Nuclear Information System (INIS)

1981-01-01

Questions have been raised concerning the ability of the drillers of the exploratory shaft for the Basalt Waste Isolation program to develop an effective seal against water inflow down the annular space between the shaft casing and shaft wall into the mined chambers. We understand the need for shaft integrity and the concern of those responsible for the shaft planning. The purpose of this report is to give documentation to allay the fears of those who may have questions remaining in their minds concerning the prospects for a dry shaft. Included in this report are discussions of five projects where shaft sealing was effective in drilled shafts and one project where the material which is recommended for the exploratory shaft was used effectively in a conventional shaft. Also discussed is the recommended multitier approach toward shaft sealing which will, if adopted, use all of the current state of the art techniques to assure the watertightness of the shaft. It should be pointed out that none of the projects described here used all of the safeguards which are recommended in this program. If any of the materials and procedures recommended here are omitted, then of course the possibility increases for water migration through the casing-borehole annulus. It is our considered opinion that if the program recommended is adopted there will be no water inflow into the shaft, but if we are wrong or if, through human error, the program is not executed correctly, that we have devices and procedures available to us which will facilitate remedial work to perfect seal in the shaft. 4 refs., 3 figs., 1 tab

RELAP/FRAP-T6 analysis of seized and sheared shaft accidents

International Nuclear Information System (INIS)

Bollinger, J.S.; Ito, T.; Peeler, G.B.

1984-01-01

Argonne National Laboratory (ANL) performed audit calculations of a Reactor Coolant Pump (RCP) seized/sheared shaft transient for the Westinghouse Seabrook Plant using RELAP5/MOD 1.5 (Cycle 32) and FRAP-T6. The objective was to determine the effect of time of loss of offsite power and other single component failures on the peak clad temperature. The RCP shaft seizure event was modeled in RELAP5 by using the pump model shaft stop option. In modeling the sheared shaft failure, the faulted pump was replaced with a branch component having no flow losses. In general, the RELAP5-predicted system response for the seized shaft transient was very comparable to the results presented in the Seabrook FSAR, although the Reactor Coolant System (RCS) pressure response was somewhat different. The RELAP5 sheared-shaft analysis results were very similar to those for the seized shaft

Seroprevalence of antibodies to diphtheria, tetanus and pertussis among healthy adolescents and adults in Iran.

Science.gov (United States)

Pourakbari, Babak; Moradi, Behnaz; Mirzaee, Farin; Mahmoudi, Shima; Teymuri, Mostafa; Mamishi, Setareh

2013-01-01

Serologic data on diseases that are preventable by vaccine are useful to evaluate the success of immunization programs. In this study we evaluated the serologic levels of antibodies to diphtheria, tetanus, and pertussis. In a cross sectional study, a total of 360 people aged 10-25 years were randomly selected and classified by sex and age (10-14, 15-20, 21-25 years). Overall, 78.8% of people aged 10-25 years had fully protected levels of diphtheria antibody (> or = 0.1 IU/ML), and 89.7% had fully protected levels of tetanus antibody (> or = 0.1 IU/ML), 94.3% of women aged 15-25 years had anti tetanus antibody sufficient to protect against neonatal tetanus (> or = 0.1 IU/ML). Antibodies to Pertussis toxin (PT) were found in 44.2% samples but only 1.4% had fully protective levels. Antibodies to PT increased with age, ranging from 33.5% in aged 10-14 years to 54.6 % in aged 21-25 years. No differences were found between male and female, except for diphtheria in age group 21-25 years. Results of this study reveal that diphtheria and tetanus (dT) are efficient between booster doses. About pertussis, most people are susceptible to pertussis and increased PT antibodies with age suggest acquired asymptomatic Bordeella pertussis infection. Also B. pertussis infections in adolescents and adults are of concern, as they are the most important source of transmission of pertussis to young, unprotected infants. So one booster dose in adolescents and adults (as CDC recommended), to reduce mortality and morbidity in infants, is therefore suggested.

Construction features of the exploratory shaft at Yucca Mountain

International Nuclear Information System (INIS)

Adair, G.W.; Fiore, J.N.

1984-01-01

The Exploratory Shaft (ES) at Yucca Mountain is planned to be constructed during 1985 and 1986 as part of the detailed site characterization for one of three sites which may be selected as candidates for location of a high-level radioactive waste repository. Conventional mining methods will be used for the shaft sinking phase of the ES project. The ES will be comprised of surface support facilities, a 1480-ft-deep circular shaft lined with concrete to a finished inside diameter of 12 ft, lateral excavations and test installations extending up to 200 ft from the shaft, and long lateral borings extending up to 2300 ft from the shaft. The estimated time for sinking the shaft to a total depth of about 1480 ft and completing the lateral excavations and borings is about two years. The major underground development planned for the primary test level at a depth of 1200 ft consists of the equivalent of 1150 ft of 15- by 15-ft drift. The total volume of rock to be removed from the shaft proper and the lateral excavations totals about 1/2 million cubic feet. Construction equipment for the shaft and underground excavation phases consists of conventional mine hoisting equipment, shot hole and rock bolt drilling jumbos, mucking machines, and hauling machines. The desire to maintain relatively uniform and even walls in selected shaft and drift intervals will require that controlled blasting techniques be employed. Certain lateral boring operations associated with tests to be conducted in the underground development may pose some unusual problems or require specialized equipment. One of the operations is boring and lining a 30-in.-diam by 600-ft-long horizontal hole with a boring machine being developed under the direction of Sandia National Laboratories. Another special operation is coring long lateral holes (500 to 2000 ft) with minimum use of liquid circulating fluids. 8 figures

Low tetanus, diphtheria and acellular pertussis (Tdap) vaccination coverage among HIV infected individuals in Austria.

Science.gov (United States)

Grabmeier-Pfistershammer, K; Herkner, H; Touzeau-Roemer, V; Rieger, A; Burgmann, H; Poeppl, W

2015-07-31

Current management guidelines of HIV infected adults include recommendation to immunization against common vaccine preventable diseases. This effort is hindered by the scarce knowledge regarding the immunization status of this especially vulnerable patient group. This study analyzed the serostatus for pertussis, diphtheria and tetanus of more than 700 HIV infected individuals residing in Austria. These individuals were representative for the Austrian HIV cohort regarding sex, age, transmission risk and HIV progression markers. Overall, 73.6% were on suppressive HAART, mean CD4 cell count was 603c/μl. Seropositivity was 84% for diphtheria, 51% for tetanus and 1% for pertussis. Migrants had a lower chance of tetanus seropositivity (OR 0.30 (CI 0.21 to 0.43)). Increase in CDC classification were associated with increased diphtheria seropositivity (OR 1.42 (CI 1.02 to 1.98)) and a CD4 nadir200c/μl, 95% lacked seroprotection to at least one of the antigens included in the triple vaccine Tdap and could be vaccinated. Thus, a proactive approach would largely reduce the number of patients at risk for these vaccine-preventable diseases. Copyright © 2015 Elsevier Ltd. All rights reserved.

DECOVALEX II project. Nirex RCF Shaft Excavation Task 1C - Coupled hydro-mechanical effects of shaft sinking within Sector 7

International Nuclear Information System (INIS)

Hakami, H.

1999-12-01

Within the framework for an international co-operation in the field of geohydrological and mechanical processes associated with radioactive waste disposal deep in rock masses (DECOVALEX II), the present work involved a number of numerical investigations in order to gain an understanding of the consequences a shaft sinking at Sellafield, England, may bring about. Research groups from five countries approached the modelling of the shaft sinking in the rock mass in question with different numerical methods. Both continuum as well as discontinuum representations of the rock mass were made. Itasca chose the code FLAC 3D , a three dimensional finite difference based computer code to carry out the numerical analyses necessary. As a first approach, an equivalent material model was chosen where discontinuities at all levels assumed to have smeared out in a rock matrix, to produce a theoretical material that would behave elasto-plastically under loading. By selecting the Mohr-Coulomb failure criterion, numerical analyses were carried out that depicted the perturbations in stress and deformational field, the shaft sinking would produce. The sinking of the shaft disturbs the flow regime of the rock mass. By presuming a number of assumptions, the approximate discharge into selected sections of the shaft were computed. Also, by numerically 'monitoring' the pore pressure drawdowns in two boreholes in close vicinity of the shaft the effect of the shaft sinking on the flow was evaluated. Having evaluated the outcome of the first series of the numerical analyses, improvements were made in the model set-up, by introducing three major flow zones with enhanced porosity and permeability in a grid that was otherwise a low conductive medium with very low porosity. A new set of flow analyses were carried out that typified the effect of the added flow zones on the general characteristics of flow in the rock mass in question. Also, by introducing a new failure criterion emerged from both

DECOVALEX II project. Nirex RCF Shaft Excavation Task 1C - Coupled hydro-mechanical effects of shaft sinking within Sector 7

Energy Technology Data Exchange (ETDEWEB)

Hakami, H. [Itasca Geomekanik AB, Stockholm (Sweden)

1999-12-01

Within the framework for an international co-operation in the field of geohydrological and mechanical processes associated with radioactive waste disposal deep in rock masses (DECOVALEX II), the present work involved a number of numerical investigations in order to gain an understanding of the consequences a shaft sinking at Sellafield, England, may bring about. Research groups from five countries approached the modelling of the shaft sinking in the rock mass in question with different numerical methods. Both continuum as well as discontinuum representations of the rock mass were made. Itasca chose the code FLAC{sup 3D}, a three dimensional finite difference based computer code to carry out the numerical analyses necessary. As a first approach, an equivalent material model was chosen where discontinuities at all levels assumed to have smeared out in a rock matrix, to produce a theoretical material that would behave elasto-plastically under loading. By selecting the Mohr-Coulomb failure criterion, numerical analyses were carried out that depicted the perturbations in stress and deformational field, the shaft sinking would produce. The sinking of the shaft disturbs the flow regime of the rock mass. By presuming a number of assumptions, the approximate discharge into selected sections of the shaft were computed. Also, by numerically 'monitoring' the pore pressure drawdowns in two boreholes in close vicinity of the shaft the effect of the shaft sinking on the flow was evaluated. Having evaluated the outcome of the first series of the numerical analyses, improvements were made in the model set-up, by introducing three major flow zones with enhanced porosity and permeability in a grid that was otherwise a low conductive medium with very low porosity. A new set of flow analyses were carried out that typified the effect of the added flow zones on the general characteristics of flow in the rock mass in question. Also, by introducing a new failure criterion emerged

Design and Delivery of HMT Half-Shaft Prototype

Science.gov (United States)

2012-11-01

spindle welded to the outer joint output is ease of Design  and Delivery of HMT Half‐ Shaft  Prototype    24    assembly. Flange 1 contains threaded... spindle , and splined shafts . Also, the spindle of the production design is splined to match the splines of the hub internals. 2.2. Analysis The...inner-joint (Figure 33). Design  and Delivery of HMT Half‐ Shaft  Prototype    27      Figure 33: FBD of Flange/ Spindle Applying Newton’s Laws to the

Study on Manufacturing Process of Hollow Main Shaft by Open Die Forging

International Nuclear Information System (INIS)

Kwon, Yong Chul; Kang, Jong Hun; Kim, Sang Sik

2016-01-01

The main shaft is one of the key components connecting the rotor hub and gear box of a wind power generator. Typically, main shafts are manufactured by open die forging method. However, the main shaft for large MW class wind generators is designed to be hollow in order to reduce the weight. Additionally, the main shafts are manufactured by a casting process. This study aims to develop a manufacturing process for hollow main shafts by the open die forging method. The design of a forging process for a solid main shaft and hollow shaft was prepared by an open die forging process design scheme. Finite element analyses were performed to obtain the flow stress by a hot compression test at different temperature and strain rates. The control parameters of each forging process, such as temperature and effective strain, were obtained and compared to predict the suitability of the hollow main shaft forging process. Finally, high productivity reflecting material utilization ratio, internal quality, shape, and dimension was verified by the prototypes manufactured by the proposed forging process for hollow main shafts

Study on Manufacturing Process of Hollow Main Shaft by Open Die Forging

Energy Technology Data Exchange (ETDEWEB)

Kwon, Yong Chul [Gyeongnam Technopark, Changwon (Korea, Republic of); Kang, Jong Hun [Jungwon Univ., Goisan (Korea, Republic of); Kim, Sang Sik [Gyeongsang Natiional Univ., Jinju (Korea, Republic of)

2016-02-15

The main shaft is one of the key components connecting the rotor hub and gear box of a wind power generator. Typically, main shafts are manufactured by open die forging method. However, the main shaft for large MW class wind generators is designed to be hollow in order to reduce the weight. Additionally, the main shafts are manufactured by a casting process. This study aims to develop a manufacturing process for hollow main shafts by the open die forging method. The design of a forging process for a solid main shaft and hollow shaft was prepared by an open die forging process design scheme. Finite element analyses were performed to obtain the flow stress by a hot compression test at different temperature and strain rates. The control parameters of each forging process, such as temperature and effective strain, were obtained and compared to predict the suitability of the hollow main shaft forging process. Finally, high productivity reflecting material utilization ratio, internal quality, shape, and dimension was verified by the prototypes manufactured by the proposed forging process for hollow main shafts.

Proceedings of the conference on shaft drilling technology

International Nuclear Information System (INIS)

Anon.

1990-01-01

This book contains the following topics, Market analysis, World-wide operations, Innovative drilling and boring, Raise boring, Shaft lining and fittings, Entry considerations for the Yucca Mountain exploratory shaft facility for potential Radioactive Waste Disposal, Drilling rigs in the coal industry

Health monitoring system for transmission shafts based on adaptive parameter identification

Science.gov (United States)

Souflas, I.; Pezouvanis, A.; Ebrahimi, K. M.

2018-05-01

A health monitoring system for a transmission shaft is proposed. The solution is based on the real-time identification of the physical characteristics of the transmission shaft i.e. stiffness and damping coefficients, by using a physical oriented model and linear recursive identification. The efficacy of the suggested condition monitoring system is demonstrated on a prototype transient engine testing facility equipped with a transmission shaft capable of varying its physical properties. Simulation studies reveal that coupling shaft faults can be detected and isolated using the proposed condition monitoring system. Besides, the performance of various recursive identification algorithms is addressed. The results of this work recommend that the health status of engine dynamometer shafts can be monitored using a simple lumped-parameter shaft model and a linear recursive identification algorithm which makes the concept practically viable.

Real-time monitoring of wind turbine generator shaft alignment using laser measurement.

OpenAIRE

Mankowski, O.; Wang, Q.

2013-01-01

Shaft Misalignment is one of the most common sources of trouble of wind turbine drive train when rigid couplings connect the shafts. Ideal alignment of the shaft is difficult to be obtained and the couplings attached to the shaft may present angular or parallel misalignment defined also as lateral and axially misalignment. Despite misalignment is often observed in the practice, there are relatively few studies on wind turbine shaft misalignment in the literature and their results are sometime...

Mine-shaft conveyance monitoring

Energy Technology Data Exchange (ETDEWEB)

Beus, M.J.; Ruff, T.M.; Iverson, S.; McCoy, W.G. [National Institute for Occupational Safety and Health, Spokane, WA (USA). Spokane Research Laboratory

2000-10-01

Monitoring conveyance position and wire rope load directly from the skip or cage top offers several significant safety and production advantages. The Spokane Research Laboratory (SRL) of the National Institute for Occupational Safety and Health (NIOSH) developed a shaft conveyance monitoring system (SCMS). This system consists of position and guide-displacement sensors, a maintenance-free battery power supply and a new sensor, which is mounted on the wire rope with a Crosby Clip, to measure hoist-rope tension. A radio data link transmits sensor output to the hoist room. A state-of-the-art automated hoisting test facility was also constructed to test the concept in a controlled laboratory setting. Field tests are now underway at the SRL hoisting research facility and in deep mine shafts in northern Idaho. 4 refs., 5 figs.

Performance of meta power rotor shaft torque meter

DEFF Research Database (Denmark)

Schmidt Paulsen, U.

2002-01-01

The present report describes the novel experimental facility in detecting shaft torque in the transmission system (main rotor shaft, exit stage of gearbox) of a wind turbine, the results and the perspectives in using this concept. The measurements arecompared with measurements, based on existing ...

WIPP air-intake shaft disturbed-rock zone study

International Nuclear Information System (INIS)

Dale, T.; Hurtado, L.D.

1996-01-01

The disturbed-rock zone surrounding the air-intake shaft at the Waste Isolation Pilot Plant (WIPP) site was investigated to determine the extent and the permeability of the disturbed-rock zone as a function of radial distance from the 6.1 m diameter shaft, at different elevations within the Salado. Gas- and brine-permeability tests were performed in the bedded halite of the Salado formation at two levels within the air-intake shaft. The gas- and brine-permeability test results demonstrated that the radial distance to an undisturbed formation permeability of 1 x 10 -21 m 2 was less than 3.0 m

Conceptual design of shaft seals for a nuclear waste disposal vault

Energy Technology Data Exchange (ETDEWEB)

NONE

1993-04-01

The concept of a disposal vault in the Canadian Shield for the effective isolation of nuclear fuel wastes is being assessed as part of the Canadian Nuclear Fuel Waste Management Program. The vault would be accessed from the surface by a number of shafts, which would likely penetrate the vault environment and intersect significant rock fractures and thereby form preferential pathways for the migration of radionuclides from the disposal area to the biosphere. Golder Associates were retained to conduct a conceptual design study of sealing and backfilling the shafts. The first volume of this report reviews current shaft sinking and lining technologies, and recommends the preferred construction methods for the shafts. Factors that could affect the design of a shaft seal system are reviewed, and a conceptual shaft seal is proposed. The second volume addresses the performance assessment of a shaft seal system. While there are no specific performance criteria against which to compare the anticipated containment characteristics of the shaft seal system proposed, the methodology developed for the performance assessment of the reference design should enable the design to be modified to meet performance criteria as they are developed. The report estimates that it will cost $133.7 million in 1986 Canadian dollars to seal three reference shafts, including $18 million for labour and equipment, $103.4 million for backfill and sealing materials, $9.5 million for project indirect costs, and $2.8 million project management. (author). 53 refs., 36 tabs., 43 figs.

Conceptual design of shaft seals for a nuclear waste disposal vault

International Nuclear Information System (INIS)

1993-04-01

The concept of a disposal vault in the Canadian Shield for the effective isolation of nuclear fuel wastes is being assessed as part of the Canadian Nuclear Fuel Waste Management Program. The vault would be accessed from the surface by a number of shafts, which would likely penetrate the vault environment and intersect significant rock fractures and thereby form preferential pathways for the migration of radionuclides from the disposal area to the biosphere. Golder Associates were retained to conduct a conceptual design study of sealing and backfilling the shafts. The first volume of this report reviews current shaft sinking and lining technologies, and recommends the preferred construction methods for the shafts. Factors that could affect the design of a shaft seal system are reviewed, and a conceptual shaft seal is proposed. The second volume addresses the performance assessment of a shaft seal system. While there are no specific performance criteria against which to compare the anticipated containment characteristics of the shaft seal system proposed, the methodology developed for the performance assessment of the reference design should enable the design to be modified to meet performance criteria as they are developed. The report estimates that it will cost $133.7 million in 1986 Canadian dollars to seal three reference shafts, including $18 million for labour and equipment, $103.4 million for backfill and sealing materials, $9.5 million for project indirect costs, and $2.8 million project management. (author). 53 refs., 36 tabs., 43 figs

Malaria chemoprophylaxis and the serologic response to measles and diphtheria-tetanus-whole-cell pertussis vaccines

Directory of Open Access Journals (Sweden)

Saliou Pierre

2005-11-01

Full Text Available Abstract Background Acute malaria has been associated with a decreased antibody response to tetanus and diphtheria toxoids, meningococcal, salmonella, and Hib vaccines. Interest in giving malaria drug therapy and prevention at the time of childhood immunizations has increased greatly following recent trials of intermittent preventive therapy during infancy (IPTi, stimulating this re-analysis of unpublished data. The effect of malaria chemoprophylaxis on vaccine response was studied following administration of measles vaccines and diphtheria-tetanus-whole cell pertussis (DTP vaccines. Methods In 1975, six villages divided into two groups of children ≤74 months of age from Burkina Faso, were assigned to receive amodiaquine hydrochloride chemoprophylaxis (CH+ every two weeks for seven months or no chemoprophylaxis (CH-. After five months, children in each group received either one dose of measles or two doses of DTP vaccines. Results For recipients of the measles vaccine, the seroconversion rates in CH+ and CH- children, respectively, were 93% and 96% (P > 0.05. The seroresponse rates in CH+ and CH- children respectively, were 73% and 86% for diphtheria (P > 0.05 and 77% and 91% for tetanus toxoid (P > 0.05. In a subset analysis, in which only children who strictly adhered to chemoprophylaxis criteria were included, there were, likewise, no significant differences in seroconversion or seroresponse for measles, diphtheria, or tetanus vaccines (P > 0.05. While analysis for pertussis showed a 43% (CH+ and 67% (CH- response (P Conclusion Malaria chemoprophylaxis prior to vaccination in malaria endemic settings did not improve or impair immunogenicity of DTP and measles vaccines. This is the first human study to look at the association between malaria chemoprophylaxis and the serologic response to whole-cell pertussis vaccine.

Grinding Method and Error Analysis of Eccentric Shaft Parts

Science.gov (United States)

Wang, Zhiming; Han, Qiushi; Li, Qiguang; Peng, Baoying; Li, Weihua

2017-12-01

RV reducer and various mechanical transmission parts are widely used in eccentric shaft parts, The demand of precision grinding technology for eccentric shaft parts now, In this paper, the model of X-C linkage relation of eccentric shaft grinding is studied; By inversion method, the contour curve of the wheel envelope is deduced, and the distance from the center of eccentric circle is constant. The simulation software of eccentric shaft grinding is developed, the correctness of the model is proved, the influence of the X-axis feed error, the C-axis feed error and the wheel radius error on the grinding process is analyzed, and the corresponding error calculation model is proposed. The simulation analysis is carried out to provide the basis for the contour error compensation.

Recent quality of ultra large rotor shafts

International Nuclear Information System (INIS)

Suzuki, Akira; Kinoshita, Shushi; Morita, Kikuo; Kikuchi, Hideo; Takada, Masayoshi

1983-01-01

Large size and high quality are required for rotor shafts accompanying recent trend of thermal and nuclear power generation toward large capacity. As for the low pressure rotor shafts for large capacity turbines, the disks and a shaft tend to be made into one body instead of conventional shrink fit construction, because of the experience of rotor accidents and the improvement of reliability. Therefore the ingots required become more and more large, and excellent production techniques are required for steel making, forging and heat treatment. Kobe Steel Ltd. have made about 20 large generator shafts from 420 t and 500 t ingots, and confirmed their stable high quality. Also a one-body low pressure rotor of 2600 mm diameter was made for trial, and its quality was examined. It was confirmed that the effect of forging and heat treatment was given sufficiently, and the production techniques for super-large one-body rotors were established. In steel making, vacuum degassing was applied twice to decrease hydrogen content, and VV restriction forging and pre-stage treatment were carried out. The properties of large rotors are reported. (Kako, I.)

TNX/HLW Long Shaft Pumps 1995-2000

International Nuclear Information System (INIS)

VanPelt, B.

2002-01-01

Problems with long shaft pumps are becoming clearer due to increased use, better instrumentation, more analysis, and increased testing activity. The problems are with reliability and not with hydraulic performance. The root cause of reliability problems is usually excessive vibration caused by design. The outlook for satisfactory pumps is improved as understanding of problems increases. Promising developments are emerging such as the tilt pad bearing. Alternative configurations, such as gas filled columns and submerged motor pumps, will require development. Continued development, in general, should be expected due to changing technology and industry changes. This report describes thirteen distinct pump programs starting with leakage of original mixer pumps in the 1980s and ending with the testing of tilt pad bearings now in progress. Eight of the programs occurred from 1996 to 2000. All involve long shaft pumps; all involve testing at TNX; and all involve a problem of some kind. The co mmon technical issue among the activities is vibration and shaft (or rotor) instability due to journal bearings. In every case, excessive shaft vibration is a reasonable and probable explanation for some or all of the problems

Immunogenicity and safety of the new reduced-dose tetanus-diphtheria vaccine in healthy Korean adolescents: A comparative active control, double-blind, randomized, multicenter phase III study.

Science.gov (United States)

Han, Seung Beom; Rhim, Jung-Woo; Shin, Hye Jo; Kim, Sang Yong; Kim, Jong-Hyun; Kim, Hyun-Hee; Lee, Kyung-Yil; Kim, Hwang Min; Choi, Young Youn; Ma, Sang Hyuk; Kim, Chun Soo; Kim, Dong Ho; Ahn, Dong Ho; Kang, Jin Han

2017-04-01

A new reduced-dose tetanus-diphtheria (Td) vaccine was developed in Korea, and phase I and II clinical trials were successfully undertaken. We conducted this double-blind, randomized, multicenter phase III clinical trial to assess the immunogenicity and safety of the new Td vaccine. Healthy adolescents 11-12 years of age were enrolled and randomized to receive the new Td vaccine (study group) or a commercially available Td vaccine (control group). Blood samples were collected prior to and 4 weeks after the vaccination. Between the study and control groups, seroprotection rate, booster response, and geometric mean titer of antibodies against diphtheria and tetanus toxoids were compared after the vaccination. All solicited and unsolicited adverse events and serious adverse events during the 6-week study period were monitored. A total of 164 adolescents received vaccination, and 156 of them were evaluated to assess immunogenicity. The seroprotection rate and geometric mean titer for antibodies against diphtheria were significantly higher in the study group, whereas those against tetanus were significantly higher in the control group. However, all seroprotection rates against diphtheria and tetanus in the study and control groups were high: 100% against diphtheria and tetanus in the study group, and 98.7% against diphtheria and 100% against tetanus in the control group. No significant differences in the frequency of solicited and unsolicited adverse events were observed between the two vaccine groups. The new Td vaccine is highly immunogenic and safe, and this new Td vaccine can be effectively used for preventing diphtheria and tetanus. Copyright © 2015. Published by Elsevier B.V.

New endoscope shaft for endoscopic transsphenoidal pituitary surgery.

NARCIS (Netherlands)

Lindert, E.J. van; Grotenhuis, J.A.

2005-01-01

OBJECTIVE: To describe a new endoscope shaft developed for suction-aspiration during endoscopic transsphenoidal pituitary surgery. METHODS: A custom-made shaft for a Wolf endoscope (Richard Wolf GmbH, Knittlingen, Germany) was developed with a height of 10 mm and a width of 5 mm, allowing an

Improved circumferential shaft seal

Science.gov (United States)

Ludwig, L. P.; Strom, T. N.

1974-01-01

Comparative tests of modified and unmodified carbon ring seals showed that addition of helical grooves to conventional segmented carbon ring seals reduced leakage significantly. Modified seal was insensitive to shaft runout and to flooding by lubricant.

Diphtheria, pertussis, and tetanus: evidence-based management of pediatric patients in the emergency department [digest].

Science.gov (United States)

Zibners, Lara; Chaudhari, Pradip

2017-02-22

Diphtheria, pertussis, and tetanus are potentially deadly bacterial infections that are largely preventable through vaccination, though they remain in the population. This issue reviews the epidemiology, pathophysiology, diagnosis, and current recommended emergency management of these conditions. Disease-specific medications, as well as treatment of the secondary complications, are examined in light of the best current evidence. Resources include obtaining diphtheria antitoxin from the United States Centers for Disease Control and Prevention and best-practice recommendations with regard to testing, involvement of government health agencies, isolation of the patient, and identification and treatment of close contacts. Most importantly, issues regarding vaccination and prevention are highlighted. [Points & Pearls is a digest of Pediatric Emergency Medicine Practice].

Torsional Vibration of a Shafting System under Electrical Disturbances

Directory of Open Access Journals (Sweden)

Ling Xiang

2012-01-01

Full Text Available Torsional vibration responses of a nonlinear shafting system are studied by a modified Riccati torsional transfer matrix combining with the Newmark-β method. Firstly, the system is modeled as a chain consisting of an elastic spring with concentrated mass points, from which a multi-segment lumped mass model is established. Secondly, accumulated errors are eliminated from the eigenfrequencies and responses of the system's torsional vibration by this newly developed procedure. The incremental transfer matrix method, combining the modified Riccati torsional transfer matrix with Newmark-β method, is further applied to solve the dynamical equations for the torsional vibration of the nonlinear shafting system. Lastly, the shafting system of a turbine-generator is employed as an illustrating example, and simulation analysis has been performed on the transient responses of the shaft's torsional vibrations during typical power network disturbances, such as three-phase short circuit, two-phase short circuit and asynchronous juxtaposition. The results validate the present method and are instructive for the design of a turbo-generator shaft.

FIXTURING DEVICE FOR DRILLING A STRAIGHT SHAFT

Directory of Open Access Journals (Sweden)

SUSAC, Florin

2017-05-01

Full Text Available The paper presents a fixturing device used for machining by drilling a straight shaft. The shaft was manufactured on EMCO CONCEPT TURN 55 CNC. The blank used was a bar with circular cross-section. The orientation and fixing scheme of the part and the orientation elements for fixturing device are presented as they were drawn in Autodesk Inventor and AutoCAD software.

Low seroprevalence of diphtheria, tetanus and pertussis in ambulatory adult patients: the need for lifelong vaccination.

Science.gov (United States)

Tanriover, Mine Durusu; Soyler, Canan; Ascioglu, Sibel; Cankurtaran, Mustafa; Unal, Serhat

2014-07-01

Tetanus, diphtheria, pertussis and measles are vaccine preventable diseases that have been reported to cause morbidity and mortality in adult population in the recent years. We aimed to document the seropositivity rates and vaccination indication for these four vaccine preventable diseases among adult and elderly patients who were seen as outpatients in a university hospital. Blood samples for tetanus, diphtheria, pertussis and measles antibodies were obtained. Results were evaluated with regards to protection levels and booster vaccine indications according to the cut-off values. A total of 1367 patients consented for the study and 1303 blood samples were available for analysis at the end of the study. The antibody levels against measles conferred protection in 98% of patients. However, 65% of the patients had no protection for diphtheria, 69% had no protection for tetanus and 90% of the patients had no protection for pertussis. Only 1.3% of the study population had seropositivity against three of the diseases-Tdap booster was indicated in 98.7%. Multivariable logistic regression showed that tetanus protection decreased with increasing age. Having a chronic disease was associated with a lower rate of protective antibodies for pertussis. We demonstrated very low rates of protection against three of the vaccine preventable diseases of childhood-diphtheria, pertussis and tetanus. Booster vaccinations are required in adult life in accordance with national and international adult vaccination guidelines. The concept of "lifelong vaccination" should be implemented and every encounter with the patient should be regarded as a chance for catch-up. Copyright © 2014 European Federation of Internal Medicine. Published by Elsevier B.V. All rights reserved.

Resurgence of Diphtheria in North Kerala, India, 2016: Laboratory Supported Case-Based Surveillance Outcomes

Directory of Open Access Journals (Sweden)

Lucky Sangal

2017-08-01

Full Text Available IntroductionAs part of national program, laboratory supported vaccine preventable diseases surveillance was initiated in Kerala in 2015. Mechanisms have been strengthened for case investigation, reporting, and data management. Specimens collected and sent to state and reference laboratories for confirmation and molecular surveillance. The major objective of this study is to understand the epidemiological information generated through surveillance system and its utilization for action.MethodsSurveillance data captured from reporting register, case investigation forms, and laboratory reports was analyzed. Cases were allotted unique ID and no personal identifying information was used for analysis. Throat swabs were collected from investigated cases as part of surveillance system. All Corynebacterium diphtheriae isolates were confirmed with standard biochemical tests, ELEK’s test, and real-time PCR. Isolates were characterized using whole genome-based multi locus sequence typing method. Case investigation forms and laboratory results were recorded electronically. Public health response by government was also reviewed.ResultsA total of 533 cases were identified in 11 districts of Kerala in 2016, of which 92% occurred in 3 districts of north Kerala; Malappuram, Kozhikode, and Kannur. Almost 79% cases occurred in >10 years age group. In <18 years age group, 62% were male while in ≥18 years, 69% were females. In <10 years age group, 31% children had received three doses of diphtheria vaccine, whereas in ≥10 years, 3% cases had received all doses. Fifteen toxigenic C. diphtheriae isolates represented 6 novel sequence types (STs (ST-405, ST-408, ST-466, ST-468, ST-469, and ST-470. Other STs observed are ST-50, ST-295, and ST-377.ConclusionDiphtheria being an emerging pathogen, establishing quality surveillance for providing real-time information on disease occurrence and mortality is imperative. The epidemiological data thus generated was

Coupling with concentric contact around motor shaft for line start synchronous motor

Science.gov (United States)

Melfi, Michael J.; Burdeshaw, Galen E.

2017-10-03

A method comprises providing a line-start synchronous motor. The motor has a stator, a rotor core disposed within the stator, and a motor shaft. In accordance with a step of the method, a coupling for coupling a load to the motor is provided. The coupling has a motor shaft attachment portion configured to provide substantially concentric contact around the shaft at the end of the motor shaft. The coupling has a load attachment portion configured to operatively connect to a load. In accordance with a step of the method, a load is coupled to the motor with the coupling, and driven from start to at least near synchronous speed during steady state operation of the motor with a load coupled thereto. The motor shaft attachment portion may comprise a bushing assembly with matching and opposed tapered surfaces that cooperate to secure the motor shaft attachment portion around the motor shaft.

Waste and dust utilisation in shaft furnaces

Energy Technology Data Exchange (ETDEWEB)

Senk, D.; Babich, A.; Gudenau, H.W. [Rhein Westfal TH Aachen, Aachen (Germany)

2005-07-01

Wastes and dusts from steel industry, non-ferrous metallurgy and other branches can be utilised e.g. in agglomeration processes (sintering, pelletising or briquetting) and by injection into shaft furnaces. This paper deals with the second way. Combustion and reduction behaviour of iron- and carbon-rich metallurgical dusts and sludges containing lead, zinc and alkali as well as other wastes with and without pulverised coal (PC) has been studied when injecting into shaft furnaces. Following shaft furnaces have been examined: blast furnace, cupola furnace, OxiCup furnace and imperial-smelting furnace. Investigations have been done at laboratory and industrial scale. Some dusts and wastes under certain conditions can be not only reused but can also improve combustion efficiency at the tuyeres as well as furnace performance and productivity.

Deep shaft high rate aerobic digestion: laboratory and pilot plant performance

Energy Technology Data Exchange (ETDEWEB)

Tran, F; Gannon, D

1981-01-01

The Deep Shaft is essentially an air-lift reactor, sunk deep in the ground (100-160 m); the resulting high hydrostatic pressure together with very efficient mixing in the shaft provide extremely high O transfer efficiencies (O.T.E.) of less than or equal to 90% vs. 4-20% in other aerators. This high O.T.E. suggests real potential for Deep-Shaft technology in the aerobic digestion of sludges and animal wastes: with conventional aerobic digesters an O.T.E. over 8% is extremely difficult to achieve. Laboratory and pilot plant Deep-Shaft aerobic digester studies carried out at Eco-Research's Pointe Claire, Quebec laboratories, and at the Paris, Ontario pilot Deep-Shaft digester are described.

Outbreak investigation for toxigenic Corynebacterium diphtheriae wound infections in refugees from Northeast Africa and Syria in Switzerland and Germany by whole genome sequencing.

Science.gov (United States)

Meinel, D M; Kuehl, R; Zbinden, R; Boskova, V; Garzoni, C; Fadini, D; Dolina, M; Blümel, B; Weibel, T; Tschudin-Sutter, S; Widmer, A F; Bielicki, J A; Dierig, A; Heininger, U; Konrad, R; Berger, A; Hinic, V; Goldenberger, D; Blaich, A; Stadler, T; Battegay, M; Sing, A; Egli, A

2016-12-01

Toxigenic Corynebacterium diphtheriae is an important and potentially fatal threat to patients and public health. During the current dramatic influx of refugees into Europe, our objective was to use whole genome sequencing for the characterization of a suspected outbreak of C. diphtheriae wound infections among refugees. After conventional culture, we identified C. diphtheriae using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and investigated toxigenicity by PCR. Whole genome sequencing was performed on a MiSeq Illumina with >70×coverage, 2×250 bp read length, and mapping against a reference genome. Twenty cases of cutaneous C. diphtheriae in refugees from East African countries and Syria identified between April and August 2015 were included. Patients presented with wound infections shortly after arrival in Switzerland and Germany. Toxin production was detected in 9/20 (45%) isolates. Whole genome sequencing-based typing revealed relatedness between isolates using neighbour-joining algorithms. We detected three separate clusters among epidemiologically related refugees. Although the isolates within a cluster showed strong relatedness, isolates differed by >50 nucleotide polymorphisms. Toxigenic C. diphtheriae associated wound infections are currently observed more frequently in Europe, due to refugees travelling under poor hygienic conditions. Close genetic relatedness of C. diphtheriae isolates from 20 refugees with wound infections indicates likely transmission between patients. However, the diversity within each cluster and phylogenetic time-tree analysis suggest that transmissions happened several months ago, most likely outside Europe. Whole genome sequencing offers the potential to describe outbreaks at very high resolution and is a helpful tool in infection tracking and identification of transmission routes. Copyright © 2016. Published by Elsevier Ltd.

Reactor coolant pump shaft seal stability during station blackout

International Nuclear Information System (INIS)

Rhodes, D.B.; Hill, R.C.; Wensel, R.G.

1987-05-01

Results are presented from an investigation into the behavior of Reactor Coolant Pump shaft seals during a potential station blackout (loss of all ac power) at a nuclear power plant. The investigation assumes loss of cooling to the seals and focuses on the effect of high temperature on polymer seals located in the shaft seal assemblies, and the identification of parameters having the most influence on overall hydraulic seal performance. Predicted seal failure thresholds are presented for a range of station blackout conditions and shaft seal geometries

Reactor coolant pump shaft seal stability during station blackout

Energy Technology Data Exchange (ETDEWEB)

Rhodes, D B; Hill, R C; Wensel, R G

1987-05-01

Results are presented from an investigation into the behavior of Reactor Coolant Pump shaft seals during a potential station blackout (loss of all ac power) at a nuclear power plant. The investigation assumes loss of cooling to the seals and focuses on the effect of high temperature on polymer seals located in the shaft seal assemblies, and the identification of parameters having the most influence on overall hydraulic seal performance. Predicted seal failure thresholds are presented for a range of station blackout conditions and shaft seal geometries.

Gear-shaft linkage, especially for nuclear reactor coolant pumps

International Nuclear Information System (INIS)

Delaunois, T.; Lefevre, R.

1990-01-01

The pump comprises: - inlet and outlet channels for the pumped fluid - a rotating shaft - a gear wheel mounted on the shaft by an axial locking nut which can support the axial hydraulic force - a thermal barrier above the gear wheel. A hydrostatic bearing fitted to the exterior surround of the gear wheel, the gear shaft linkage is made by at least a centering and locating device having a cylindrical span and an axial stop and another independent device which can take up the torque [fr

Classical Measurement Methods and Laser Scanning Usage in Shaft Hoist Assembly Inventory

Science.gov (United States)

Jaśkowski, Wojciech; Lipecki, Tomasz; Matwij, Wojciech; Jabłoński, Mateusz

2018-03-01

The shaft hoist assembly is the base of underground mining plant. Its efficiency and correct operation is subject to restrictive legal regulations and is controlled on a daily visual assessment by shaft crew and energomechanics. In addition, in the regular interval, the shaft hoist assembly is subject to a thorough inventory, which includes the determination of the geometrical relationships between the hoisting machine, the headframe and the shaft with its housing. Inventory measurements for shaft and headframe are used for years of conventional geodetic methods including mechanical or laser plumbing and tachymetric surveys. Additional precision levelling is also used for measuring shafts of hoisting machines and rope pulleys. Continuous modernization of measuring technology makes it possible to implement the further methods to the above mentioned purposes. The comparison of the accuracy and the economics of performing measurements based on many years of experience with comprehensive inventory of shaft hoist assembly using various research techniques was made and detailed in the article.

Classical Measurement Methods and Laser Scanning Usage in Shaft Hoist Assembly Inventory

Directory of Open Access Journals (Sweden)

Jaśkowski Wojciech

2018-01-01

Full Text Available The shaft hoist assembly is the base of underground mining plant. Its efficiency and correct operation is subject to restrictive legal regulations and is controlled on a daily visual assessment by shaft crew and energomechanics. In addition, in the regular interval, the shaft hoist assembly is subject to a thorough inventory, which includes the determination of the geometrical relationships between the hoisting machine, the headframe and the shaft with its housing. Inventory measurements for shaft and headframe are used for years of conventional geodetic methods including mechanical or laser plumbing and tachymetric surveys. Additional precision levelling is also used for measuring shafts of hoisting machines and rope pulleys. Continuous modernization of measuring technology makes it possible to implement the further methods to the above mentioned purposes. The comparison of the accuracy and the economics of performing measurements based on many years of experience with comprehensive inventory of shaft hoist assembly using various research techniques was made and detailed in the article.

Effect of centrifugal force on natural frequency of lateral vibration of rotating shafts

Science.gov (United States)

Behzad, M.; Bastami, A. R.

2004-07-01

This paper investigates the effect of shaft rotation on its natural frequency. Apart from gyroscopic effect, the axial force originated from centrifugal force and the Poisson effect results in change of shaft natural frequency. D'Alembert principle for shaft in cylindrical co-ordinate system, along with the stress-strain relation, gives the non-homogenous linear differential equation, which can be used to calculate axial stress in the shaft. Numerical results of this study show that axial stress produced by shaft rotation has a major effect on the natural frequency of long high-speed shafts, while shaft diameter has no influence on the results. In addition, change in lateral natural frequency due to gyroscopic effect is compared with the results of this study.

More than 20 years after re-emerging in the 1990s, diphtheria remains a public health problem in Latvia.

Science.gov (United States)

Kantsone, Ieva; Lucenko, Irina; Perevoscikovs, Jurijs

2016-12-01

In 1994, the World Health Organization (WHO) declared the goal of eliminating diphtheria within the WHO European Region by the year 2000. However, in 1990 an epidemic emerged within the Russian Federation and spread to other countries, including Latvia, by 1994. We describe national surveillance and immunisation coverage data in Latvia from 1994 to 2014 and present historical data from 1946. We defined a laboratory-confirmed case as a clinical case in which toxin-producing Corynebacterium diphtheriae, C. ulcerans or C. pseudotuberculosis was isolated. From 1994 to 2014, 1,515 cases were reported, giving an average annual incidence of 3.2 cases per 100,000 inhabitants (range 0.1-14.8), with the highest incidence in age groups 5-19 and 40-49 years (4.4 and 4.3/100,000, respectively); 111 deaths were reported, 83.8% cases were laboratory-confirmed. Most cases occurred in unvaccinated adults. To improve disease control a supplementary immunisation campaign for adults was initiated in 1995, and by the end of 1998 national coverage among adults reached 70%, and reached 77% in 2003, but declined to 59% by 2014. Diphtheria remains a problem in Latvia with continued circulation of toxin-producing strains of C. diphtheriae. We recommend to strengthen immunisation to cover adults, as well as the education of health professionals and a serological survey. This article is copyright of The Authors, 2016.

Method of lining a vertical mine shaft with concrete

Science.gov (United States)

Eklund, James D.; Halter, Joseph M.; Rasmussen, Donald E.; Sullivan, Robert G.; Moffat, Robert B.

1981-01-01

The apparatus includes a cylindrical retainer form spaced inwardly of the wall of the shaft by the desired thickness of the liner to be poured and having overlapping edges which seal against concrete flow but permit the form to be contracted to a smaller circumference after the liner has hardened and is self-supporting. A curb ring extends downwardly and outwardly toward the shaft wall from the bottom of the retainer form to define the bottom surface of each poured liner section. An inflatable toroid forms a seal between the curb ring and the shaft wall. A form support gripper ring having gripper shoes laterally extendable under hydraulic power to engage the shaft wall supports the retainer form, curb ring and liner until the newly poured liner section becomes self-supporting. Adjusting hydraulic cylinders permit the curb ring and retainer form to be properly aligned relative to the form support gripper ring. After a liner section is self-supporting, an advancing system advances the retainer form, curb ring and form support gripper ring toward a shaft boring machine above which the liner is being formed. The advancing system also provides correct horizontal alignment of the form support gripper ring.

Exploratory shaft conceptual design report: Permian Basin

International Nuclear Information System (INIS)

1983-07-01

This conceptual design report summarizes the conceptualized design for an exploratory shaft facility at a representative site in the Permian Basin locatd in the western part of Texas. Conceptualized designs for other possible locations (Paradox Basin in Utah and Gulf Interior Region salt domes in Louisiana and Mississippi) are summarized in separate reports. The purpose of the exploratory shaft facility is to provide access to the reference repository horizon to permit in situ testing of the salt. The in situ testing is necessary to verify repository salt design parameters, evaluate isotropy and homogeneity of the salt, and provide a demonstration of the constructability and confirmation of the design to gain access to the repository. The fundamental purpose of this conceptual design report is to assure the feasibility of the exploratory shaft project and to develop a reliable cost estimate and realistic schedule. Because a site has not been selected and site-specific subsurface data are not available, it has been necessary to make certain assumptions in order to develop a conceptual design for an exploratory shaft facility in salt. As more definitive information becomes available to support the design process, adjustments in the projected schedule and estimated costs will be required

Exploratory shaft conceptual design report: Paradox Basin

International Nuclear Information System (INIS)

1983-07-01

This conceptual design report summarizes the conceptualized design for an exploratory shaft facility at a representative site in the Paradox Basin located in the southeastern part of Utah. Conceptualized designs for other possible locations (Permian Basin in Texas and Gulf Interior Region salt domes in Louisiana and Mississippi) are summarized in separate reports. The purpose of the exploratory shaft facility is to provide access to the reference repository horizon to permit in situ testing of the salt. The in-situ testing is necessary to verify repository salt design parameters, evaluate isotropy and homogeneity of the salt, and provide a demonstration of the constructability and confirmation of the design to gain access to the repository. The fundamental purpose of this conceptual design report is to assure the feasibility of the exploratory shaft project and to develop a reliable cost estimate and realistic schedule. Because a site has not been selected and site-specific subsurface data are not available, it has been necessary to make certain assumptions in order to develop a conceptual design for an exploratory shaft facility in salt. As more definitive information becomes available to support the design process, adjustments in the projected schedule and estimated costs will be required

10 CFR 60.134 - Design of seals for shafts and boreholes.

Science.gov (United States)

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Design of seals for shafts and boreholes. 60.134 Section....134 Design of seals for shafts and boreholes. (a) General design criterion. Seals for shafts and... closure. (b) Selection of materials and placement methods. Materials and placement methods for seals shall...

Proposed design procedure for transmission shafting under fatigue loading

Science.gov (United States)

Loewenthal, S. H.

1978-01-01

The B106 American National Standards Committee is currently preparing a new standard for the design of transmission shafting. A design procedure, proposed for use in the new standard, for computing the diameter of rotating solid steel shafts under combined cyclic bending and steady torsion is presented. The formula is based on an elliptical variation of endurance strength with torque exhibited by combined stress fatigue data. Fatigue factors are cited to correct specimen bending endurance strength data for use in the shaft formula. A design example illustrates how the method is to be applied.

Device for selectively securing an object to a shaft

International Nuclear Information System (INIS)

Calizano, F.; Chauvel, M.

1984-01-01

A magnetic tape reel is secured to a reel drive motor shaft by a device including a hub secured to the shaft, a plurality of shoes, and actuating means for thrusting the shoes against the reel and for releasing them from contact with the reel. The securing device includes a cam mounted on the shaft in combination with a locking device including the cam periphery. The locking device thrusts the shoes against the object and is operated by rotation of the motor. The cam is immobilized in rotation while the shoes are locked

Air intake shaft performance tests (Shaft 5): In situ data report (May 1988--July 1995). Waste Isolation Pilot Plant (WIPP) Thermal/Structural Interactions Program

International Nuclear Information System (INIS)

Munson, D.E.; Baird, G.T.; Jones, R.L.

1995-07-01

Data are presented from the Air Intake Shaft Test, an in situ test fielded at the Waste Isolation Pilot Plant (WIPP). The construction of this shaft, well after the initial three access shafts, presented an unusual opportunity to obtain valuable detailed data on the mechanical response of a shaft for application to seal design. These data include selected fielding information, test configuration, instrumentation activities, and comprehensive results from a large number of gages. Construction of the test began in December 1987; gage data in this report cover the period from May 1988 through July 1995, with the bulk of the data obtained after obtaining access in November, 1989 and from the heavily instrumented period after remote gage installation between May, 1990, and October, 1991

Air intake shaft performance tests (Shaft 5): In situ data report (May 1988--July 1995). Waste Isolation Pilot Plant (WIPP) Thermal/Structural Interactions Program

Energy Technology Data Exchange (ETDEWEB)

Munson, D.E. [Sandia National Labs., Albuquerque, NM (United States). Repository Isolation Systems Dept.; Hoag, D.L.; Ball, J.R. [RE/SPEC Inc., Albuquerque, NM (United States); Baird, G.T.; Jones, R.L. [Tech Reps, Inc., Albuquerque, NM (United States)

1995-07-01

Data are presented from the Air Intake Shaft Test, an in situ test fielded at the Waste Isolation Pilot Plant (WIPP). The construction of this shaft, well after the initial three access shafts, presented an unusual opportunity to obtain valuable detailed data on the mechanical response of a shaft for application to seal design. These data include selected fielding information, test configuration, instrumentation activities, and comprehensive results from a large number of gages. Construction of the test began in December 1987; gage data in this report cover the period from May 1988 through July 1995, with the bulk of the data obtained after obtaining access in November, 1989 and from the heavily instrumented period after remote gage installation between May, 1990, and October, 1991.

ACTION OF DIPHTHERIA TOXIN IN THE GUINEA PIG

Science.gov (United States)

Baseman, Joel B.; Pappenheimer, A. M.; Gill, D. M.; Harper, Annabel A.

1970-01-01

The blood clearance and distribution in the tissues of 125I after intravenous injection of small doses (1.5–5 MLD or 0.08–0.25 µg) of 125I-labeled diphtheria toxin has been followed in guinea pigs and rabbits and compared with the fate of equivalent amounts of injected 125I-labeled toxoid and bovine serum albumin. Toxoid disappeared most rapidly from the blood stream and label accumulated and was retained in liver, spleen, and especially in kidney. Both toxin and BSA behaved differently. Label was found widely distributed among all the organs except the nervous system and its rate of disappearance from the tissues paralleled its disappearance from the circulation. There was no evidence for any particular affinity of toxin for muscle tissue or for a "target" organ. Previous reports by others that toxin causes specific and selective impairment of protein synthesis in muscle tissue were not confirmed. On the contrary, both in guinea pigs and rabbits, a reduced rate of protein synthesis was observed in all tissues that had taken up the toxin label. In tissues removed from intoxicated animals of both species there was an associated reduction in aminoacyl transferase 2 content. It is concluded that the primary action of diphtheria toxin in the living animal is to effect the inactivation of aminoacyl transferase 2. The resulting inhibition in rate of protein synthesis leads to morphologic damage in all tissues reached by the toxin and ultimately to death of the animal. PMID:5511567

Parameter optimization method for longitudinal vibration absorber of ship shaft system

Directory of Open Access Journals (Sweden)

LIU Jinlin

2017-05-01

Full Text Available The longitudinal vibration of the ship shaft system is the one of the most important factors of hull stern vibration, and it can be effectively minimized by installing a longitudinal vibration absorber. In this way, the vibration and noise of ships can be brought under control. However, the parameters of longitudinal vibration absorbers have a great influence on the vibration characteristics of the shaft system. As such, a certain shafting testing platform was studied as the object on which a finite model was built, and the relationship between longitudinal stiffness and longitudinal vibration in the shaft system was analyzed in a straight alignment state. Furthermore, a longitudinal damping model of the shaft system was built in which the parameters of the vibration absorber were non-dimensionalized, the weight of the vibration absorber was set as a constant, and an optimizing algorithm was used to calculate the optimized stiffness and damping coefficient of the vibration absorber. Finally, the longitudinal vibration frequency response of the shafting testing platform before and after optimizing the parameters of the longitudinal vibration absorber were compared, and the results indicated that the longitudinal vibration of the shafting testing platform was decreased effectively, which suggests that it could provide a theoretical foundation for the parameter optimization of longitudinal vibration absorbers.

A Disulfide Bond-forming Machine Is Linked to the Sortase-mediated Pilus Assembly Pathway in the Gram-positive Bacterium Actinomyces oris*

Science.gov (United States)

Reardon-Robinson, Melissa E.; Osipiuk, Jerzy; Chang, Chungyu; Wu, Chenggang; Jooya, Neda; Joachimiak, Andrzej; Das, Asis; Ton-That, Hung

2015-01-01

Export of cell surface pilins in Gram-positive bacteria likely occurs by the translocation of unfolded precursor polypeptides; however, how the unfolded pilins gain their native conformation is presently unknown. Here, we present physiological studies to demonstrate that the FimA pilin of Actinomyces oris contains two disulfide bonds. Alanine substitution of cysteine residues forming the C-terminal disulfide bridge abrogates pilus assembly, in turn eliminating biofilm formation and polymicrobial interaction. Transposon mutagenesis of A. oris yielded a mutant defective in adherence to Streptococcus oralis, and revealed the essential role of a vitamin K epoxide reductase (VKOR) gene in pilus assembly. Targeted deletion of vkor results in the same defects, which are rescued by ectopic expression of VKOR, but not a mutant containing an alanine substitution in its conserved CXXC motif. Depletion of mdbA, which encodes a membrane-bound thiol-disulfide oxidoreductase, abrogates pilus assembly and alters cell morphology. Remarkably, overexpression of MdbA or a counterpart from Corynebacterium diphtheriae, rescues the Δvkor mutant. By alkylation assays, we demonstrate that VKOR is required for MdbA reoxidation. Furthermore, crystallographic studies reveal that A. oris MdbA harbors a thioredoxin-like fold with the conserved CXXC active site. Consistently, each MdbA enzyme catalyzes proper disulfide bond formation within FimA in vitro that requires the catalytic CXXC motif. Because the majority of signal peptide-containing proteins encoded by A. oris possess multiple Cys residues, we propose that MdbA and VKOR constitute a major folding machine for the secretome of this organism. This oxidative protein folding pathway may be a common feature in Actinobacteria. PMID:26170452

A Disulfide Bond-forming Machine Is Linked to the Sortase-mediated Pilus Assembly Pathway in the Gram-positive Bacterium Actinomyces oris.

Science.gov (United States)

Reardon-Robinson, Melissa E; Osipiuk, Jerzy; Chang, Chungyu; Wu, Chenggang; Jooya, Neda; Joachimiak, Andrzej; Das, Asis; Ton-That, Hung

2015-08-28

Export of cell surface pilins in Gram-positive bacteria likely occurs by the translocation of unfolded precursor polypeptides; however, how the unfolded pilins gain their native conformation is presently unknown. Here, we present physiological studies to demonstrate that the FimA pilin of Actinomyces oris contains two disulfide bonds. Alanine substitution of cysteine residues forming the C-terminal disulfide bridge abrogates pilus assembly, in turn eliminating biofilm formation and polymicrobial interaction. Transposon mutagenesis of A. oris yielded a mutant defective in adherence to Streptococcus oralis, and revealed the essential role of a vitamin K epoxide reductase (VKOR) gene in pilus assembly. Targeted deletion of vkor results in the same defects, which are rescued by ectopic expression of VKOR, but not a mutant containing an alanine substitution in its conserved CXXC motif. Depletion of mdbA, which encodes a membrane-bound thiol-disulfide oxidoreductase, abrogates pilus assembly and alters cell morphology. Remarkably, overexpression of MdbA or a counterpart from Corynebacterium diphtheriae, rescues the Δvkor mutant. By alkylation assays, we demonstrate that VKOR is required for MdbA reoxidation. Furthermore, crystallographic studies reveal that A. oris MdbA harbors a thioredoxin-like fold with the conserved CXXC active site. Consistently, each MdbA enzyme catalyzes proper disulfide bond formation within FimA in vitro that requires the catalytic CXXC motif. Because the majority of signal peptide-containing proteins encoded by A. oris possess multiple Cys residues, we propose that MdbA and VKOR constitute a major folding machine for the secretome of this organism. This oxidative protein folding pathway may be a common feature in Actinobacteria. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Resurgence of diphtheria in rural areas of North Karnataka, India

OpenAIRE

Mahantesh V Parande; Subarna Roy; B G Mantur; Aisha M Parande; Rupali S Shinde

2017-01-01

Background: A diphtheria outbreak was identified from Vijayapura (formerly Bijapur) district in the South Indian state of Karnataka in 2011. There was a surge in the number of throat swab samples received under the Integrated Disease Surveillance Programme (IDSP) in North Karnataka since then. Objectives: A microbiological study was undertaken to generate information on the status of resurgence of the disease in the region. Materials and Methods: Throat swabs from 432 suspected cases of dipht...

Seroprevalence and Determinants of Immunity to Diphtheria for Children Living in Two Districts of Contrasting Incidence During an Outbreak in East Java, Indonesia.

Science.gov (United States)

Hughes, Gareth J; Mikhail, Amy F W; Husada, Dominicus; Irawan, Eveline; Kafatos, George; Bracebridge, Samantha; Pebody, Richard; Efstratiou, Androulla

2015-11-01

In 2012, an ongoing outbreak of diphtheria in Indonesia was focused in the province of East Java. There was a need to assess vaccine coverage and immunity gaps in children. We conducted a cross-sectional seroprevalence and vaccine coverage survey of children 1-15 years of age in 2 districts of East Java: one of high incidence (on the island of Madura) and one of low incidence (on the mainland). From each district, we sampled 150 children (10 children per year of age). Sera and throat swabs were taken to determine immunity and carriage status. Immunity was defined as ≥0.1 international unit/mL of antibody to diphtheria toxin. A total of 297 children were selected to participate in the study. Coverage of three doses of combined vaccine for diphtheria, tetanus and pertussis was significantly lower (P Java. Immunity in high incidence districts is likely because of natural immunity acquired through exposure to toxigenic C. diphtheriae. Booster vaccines are essential for achieving protective levels of immunity.

Antibody levels to tetanus, diphtheria, measles and varicella in patients with primary immunodeficiency undergoing intravenous immunoglobulin therapy: a prospective study.

Science.gov (United States)

Nobre, Fernanda Aimée; Gonzalez, Isabela Garrido da Silva; Simão, Raquel Maria; de Moraes Pinto, Maria Isabel; Costa-Carvalho, Beatriz Tavares

2014-06-21

Patients with antibody deficiencies depend on the presence of a variety of antibody specificities in intravenous immunoglobulin (IVIG) to ensure continued protection against pathogens. Few studies have examined levels of antibodies to specific pathogens in IVIG preparations and little is known about the specific antibody levels in patients under regular IVIG treatment. The current study determined the range of antibodies to tetanus, diphtheria, measles and varicella in IVIG products and the levels of these antibodies in patients undergoing IVIG treatment. We selected 21 patients with primary antibody deficiencies who were receiving regular therapy with IVIG. Over a period of one year, we collected four blood samples from each patient (every 3 months), immediately before immunoglobulin infusion. We also collected samples from the IVIG preparation the patients received the month prior to blood collection. Antibody levels to tetanus, diphtheria, measles and varicella virus were measured in plasma and IVIG samples. Total IgG levels were determined in plasma samples. Antibody levels to tetanus, diphtheria, varicella virus and measles showed considerable variation in different IVIG lots, but they were similar when compared between commercial preparations. All patients presented with protective levels of antibodies specific for tetanus, measles and varicella. Some patients had suboptimal diphtheria antibody levels. There was a significant correlation between serum and IVIG antibodies to all pathogens, except tetanus. There was a significant correlation between diphtheria and varicella antibodies with total IgG levels, but there was no significant correlation with antibodies to tetanus or measles. The study confirmed the variation in specific antibody levels between batches of the same brand of IVIG. Apart from the most common infections to which these patients are susceptible, health care providers must be aware of other vaccine preventable diseases, which still exist

Experiences with the operation of a shaft-helix in connection with a special steel ring lining in the shafts Gorleben 1 and 2

International Nuclear Information System (INIS)

Tonscheidt, H.W.; Kahl, J.

1991-01-01

When sinking the Gorleben 1 shaft good reasons prevented the method of drilling and blasting to create the excavation. Considering the special conditions and after a successful test, the application of a modified helix was given preference to an impact-ripper, tested on an other freeze shaft project. A report is given on the experiences gained with respect to the following criteria: Assembly, dismantling and transport of the machine in the shaft - conveyance of electric energy - dust problems when cutting the concrete plug and the means engaged to overcome those problems - performance in the different types of rock formation - cutting the circular shape of the excavation with sufficient accuracy - matters of safety in connection with cutting, loading and lining the excavation - consumption and costs of cutters - total costs. A final assessment of the excavation system is made with respect to its possible application with for shaft-sinking projects. (orig.) [de

Analysis and optimization of dynamic model of eccentric shaft grinder

Science.gov (United States)

Gao, Yangjie; Han, Qiushi; Li, Qiguang; Peng, Baoying

2018-04-01

Eccentric shaft servo grinder is the core equipment in the process chain of machining eccentric shaft. The establishment of the movement model and the determination of the kinematic relation of the-axis in the grinding process directly affect the quality of the grinding process, and there are many error factors in grinding, and it is very important to analyze the influence of these factors on the work piece quality. The three-dimensional model of eccentric shaft grinder is drawn by Pro/E three-dimensional drawing software, the model is imported into ANSYS Workbench Finite element analysis software, and the finite element analysis is carried out, and then the variation and parameters of each component of the bed are obtained by the modal analysis result. The natural frequencies and formations of the first six steps of the eccentric shaft grinder are obtained by modal analysis, and the weak links of the parts of the grinder are found out, and a reference improvement method is proposed for the design of the eccentric shaft grinder in the future.

Fractures of the shafts of the tibia and fibula

International Nuclear Information System (INIS)

Bender, C.E.; Campbell, D.C.

1985-01-01

Fractures of the shafts of the tibia and fibula are the most common long bone fractures. This chapter discusses tibial and fibular shaft fractures. Treatment of tibial and fibular fractures is similar and, therefore, reference is primarily made to the tibia. Diagnostic techniques are also evaluated

Controlled blasting and its implications for the NNWSI project exploratory shaft

International Nuclear Information System (INIS)

Van Eeckhout, E.M.

1987-09-01

This report reviews controlled blasting techniques for shaft sinking. Presplitting and smooth blasting are the techniques of principal interest. Smooth blasting is preferred for the Nevada Nuclear Waste Storage Investigations exploratory shaft. Shaft damage can be monitored visually or by peak velocity measurements and refractive techniques. Damage into the rock should be limited to 3 ft. 40 refs., 22 figs., 7 tabs

Design capability of CANDU heat transport pump shafts against cracking

International Nuclear Information System (INIS)

Kumar, A.N.; Sheikh, Z.B.; Padgett, A.

1993-01-01

During 1986 three different Light Water Reactors (LWR's) in the U.S. reported either a cracked or fractured shaft on one or more of their reactor coolant (RC) pumps. The RC pumps for all these stations were supplied by Byron Jackson (BJ) Pump Company. A majority of CANDU heat transport (HT) pumps (equivalent of RC pumps) are supplied by BJ Pump Company and are similar in design to RC pumps. Hence the failure of these RC pumps in the U.S. utilities caused concern regarding the relevance of these failures to the BJ supplied CANDU HT pumps (HTP). This paper presents the results of AECL assessment to establish the capability of the HT pump shaft against cracking. Two methods were used for assessment: (a) detailed comparative design review of the HTP and RCP shafts; (b) semi-empirical analysis of the HTP shafts. The results of the AECL assessment showed significant differences in detailed design, materials, assembly and fits of various components and the control of operating parameters between the HT and RC pumps. It was concluded that because of these differences the failures similar to RC pump shafts are not likely to appear in HT pump shafts. This conclusion is further reinforced by about 140,000 hours of operating history of the longest running HT pump of comparable size to RC Pumps, without failures

Nonsynchronous vibrations observed in a supercritical power transmission shaft

Science.gov (United States)

Darlow, M. S.; Zorzi, E. S.

1979-01-01

A flexible shaft is prone to a number of vibration phenomena which occur at frequencies other than synchronous with rotational speed. Nonsynchronous vibrations from several sources were observed while running a test rig designed to simulate the operation of a supercritical power transmission shaft. The test rig was run first with very light external damping and then with a higher level of external damping, for comparison. As a result, the effect of external damping on the nonsynchronous vibrations of the test rig was observed. All of these nonsynchronous vibrations were of significant amplitude. Their presence in the vibrations spectra for a supercritical power transmission shaft at various speeds in the operating range indicates that very careful attention to all of the vibration spectra should be made in any supercritical power transmission shafting. This paper presents a review of the analysis performed and a comparison with experimental data. A thorough discussion of the observed nonsynchronous whirl is also provided.

Shaft Crack Identification Based on Vibration and AE Signals

Directory of Open Access Journals (Sweden)

Wenxiu Lu

2011-01-01

Full Text Available The shaft crack is one of the main serious malfunctions that often occur in rotating machinery. However, it is difficult to locate the crack and determine the depth of the crack. In this paper, the acoustic emission (AE signal and vibration response are used to diagnose the crack. The wavelet transform is applied to AE signal to decompose into a series of time-domain signals, each of which covers a specific octave frequency band. Then an improved union method based on threshold and cross-correlation method is applied to detect the location of the shaft crack. The finite element method is used to build the model of the cracked rotor, and the crack depth is identified by comparing the vibration response of experiment and simulation. The experimental results show that the AE signal is effective and convenient to locate the shaft crack, and the vibration signal is feasible to determine the depth of shaft crack.

Application of hydraulically assembled shaft coupling hubs to large agitators

International Nuclear Information System (INIS)

Murray, W.E.; Anderson, T.D.; Bethmann, H.K.

1991-01-01

This paper describes the basis for and implementation of hydraulically assembled shaft coupling hubs for large tank-mounted agitators. This modification to the original design was intended to minimize maintenance personnel exposure to ionizing radiation and also provide for disassembly capability without damage to shafts or hubs. In addition to realizing these objectives, test confirmed that the modified couplings reduced agitator shaft end runouts approximately 65%, thereby reducing bearing loads and increasing service life, a significant enhancement for a nuclear facility. 5 refs

Natural Ventilation of Buildings through Light Shafts. Design-Based Solution Proposals

Science.gov (United States)

Ángel Padilla-Marcos, Miguel; Meiss, Alberto; Feijó-Muñoz, Jesús

2017-10-01

This work analyses how the built environment affects the quality of the air to be introduced into buildings from light shafts. Several factors such as urban environment and building design intervene in the ability of the light shaft to produce its air change process. Urban areas continuously pollute the air in cities which affects the human health and the environment sustainability. Poor air quality outside buildings supposes a big energy waste to promote an acceptable air quality inside buildings. That requires a large flow rate to maintain the indoor air quality which is translated to an energy efficiency term. The main objective focuses on the impact of standardized architecture design in the quality of the indoor air dependent on the air change in the light shaft. The air change capacity of the outdoor space is numbered analysed using the concept of air change efficiency (ACE). ACE is determined by the built environment, the wind conditions and the design of the building containing light shafts. This concept is comparatively evaluated inside a control domain virtually defined to obtain the mean age of the air for a known air volume. The longer the light shaft in the wind direction is, the better the ACE is compared with other options. Light shafts up to 12 metres high are the most suitable in order to obtain acceptable efficiency results. Other studied cases verify that assumption. Different simplified tools for the technicians to evaluate the design of buildings containing light shafts are proposed. Some strategies of architectural design of buildings with light shafts to be used for ventilation are presented.

Dot immunoassay for the simultaneous determination of postvaccination immunity against pertussis, diphtheria, and tetanus.

Science.gov (United States)

Khramtsov, Pavel; Bochkova, Maria; Timganova, Valeria; Zamorina, Svetlana; Rayev, Mikhail

2017-06-01

A dot immunoassay for simultaneous semiquantitative detection of IgG against tetanus toxoid (Ttx) and diphtheria toxoid (Dtx) and qualitative detection of anti-Bordetella pertussis IgGs in human blood serum using carbon nanoparticles functionalized with streptococcal protein G was developed. Inactivated B. pertussis cells in suspension form were used as an antigen in the immunoassay. Pertussis, tetanus, and diphtheria antigens were separately spotted onto nitrocellulose strips, and then the immunostrips were successively incubated with blood sera and a suspension of carbon nanoparticles. The immunostrips were then scanned with a flatbed scanner, and the images obtained were processed with ImageJ. One hundred fifty-five venous blood serum samples from children vaccinated with diphtheria, tetanus, and whole-cell pertussis (DTwP) vaccine were tested in comparison with a conventional ELISA and agglutination test. The total time required for analysis of 32 serum samples was less than 3 h. Comparison between the results of the dot immunoassay and the corresponding ELISA/agglutination test revealed a high level of agreement (Cohen's kappa between 0.765 and 0.813). The lower limit of quantification was 0.06 IU/ml for anti-Ttx and anti-Dtx. The intra-assay coefficients of variation were less than 15% for anti-Ttx and anti-Dtx and less than 10% for anti-pertussis. The diagnostic sensitivity of detection of the antibody protection level was 93.5% for anti-Ttx [95% confidence interval (CI) 83.5-97.9%], 92.4% for anti-Dtx (95% CI 80.9297.5%), and 90.2% for anti-pertussis (95% CI 75.9-96.8%). The diagnostic specificity was 90.9% for anti-Ttx (95% CI 57.1-99.5%), 85% for anti-Dtx (95% CI 61.1-96.0%), and 89.3% for anti-pertussis (95%CI 80.8-94.5%). The dot immunoassay developed does not require expensive reading equipment, and allows detection of antibodies against three antigens in a single analysis. The immunostrips can be stored for a long time without changes in the

46 CFR 171.100 - Shaft tunnels and stern tubes.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Shaft tunnels and stern tubes. 171.100 Section 171.100... PERTAINING TO VESSELS CARRYING PASSENGERS Additional Subdivision Requirements § 171.100 Shaft tunnels and... passengers on an international voyage. (b) The watertight seal in the bulkhead between the stern tube space...

Percutaneous Kirschner wire (K-wire) fixation for humerus shaft ...

African Journals Online (AJOL)

Background: Fractures of the humeral shaft are uncommon, representing less than 10 percent of all fractures in children. Humeral shaft fractures in children can be treated by immobilisation alone. A small number of fractures are unable to be reduced adequately or maintained in adequate alignment, and these should be ...

New Technical Solution for Vertical Shaft Equipping Using Steel Headframe of Multifunction Purpose

Science.gov (United States)

Kassikhina, Elena; Pershin, Vladimir; Glazkov, Yurij

2017-11-01

The article reviews a novel approach to the design of steel angle headframe for vertical shafts of coal and ore mines on the basis of rational design solutions. Practice of construction of coal and ore mines provides application of various designs for steel angle headframes which are divided into separate large assembly blocks and constructive elements during assembling operations. Design of these blocks and elements, their weight and dimensions effect the chose of the method of assembling on which economic and technological indicators, as well as duration of down-time, depend on during performance of construction operations in shaft. The technical solution on equipment provision for mine vertical shaft using headframe of multifunctional purpose will allow changing the management construction of vertical shaft. The constructive design of the headgear allows application of the effective method of assembly and thus to provide improvement of the technical and economic indexes, and high calendar time rate of the shaft construction due to reduction of duration of works on equipment provision for the shaft and to refurbishment of the shaft in order to carry out horizontal mining.

A field trail for sealing abandoned mine shafts and adits with lightweight concrete

International Nuclear Information System (INIS)

Skinner, E.H.; Beckett, L.A.

1994-01-01

An abandoned mine shaft near Omar, in Logan County, WV, was permanently sealed through a cooperative agreement between the West Virginia Department of Commerce, Labor, and Environmental Resources, Division of Environmental Protection, and the US Bureau of Mines (USBM), Abandoned Mine Lands (AML) Program. An engineered shaft seal design was developed and demonstrated that featured lightweight concrete as a key material component at a wet density of about 45 lb/ft 3 . A reinforced concrete cap designed for 5 psi live load was placed over the shaft seal. Applicable new concrete technologies relating to a 100-yr design life were utilized to assure future integrity of the shaft seal. Waterproofing methods were included in the shaft seal design to provide protection from ambient moisture and corrosive mine waters and to increase the long-term durability of the shaft seal. All construction methods used in the field trial are fully adaptable for the mine-reclamation contractor. The USBM research objectives were to develop a broad generic design that will be widely applicable to other adit-sealing and shaft-sealing problems throughout the mining industry

Determining basic parameters of shafts with cage hoisting systems in mines with steep seams

Energy Technology Data Exchange (ETDEWEB)

Durov, E.M.

1982-05-01

This paper analyzes problems associated with increasing depth of mine shafts in operating coal mines. Schemes of shaft excavation in mines with steep coal seams are analyzed. Removal of mine rock and the ground surface by existing mine shafts is most economical in most cases. Yuzhgiproshakht has investigated a number of hoisting schemes during mine shaft excavation in order to select the optimum shaft diameter which permits shaft reconstruction and deepening to be optimized. The following conditions are analyzed: coal output of a coal mine ranges from 0.9 megatons (Mt) to 1.8 Mt/year, mining depth ranges from 600 m to 1600 m (with intermediary depth of 800, 1000, 1200 and 1400 m also considered). Separate hoisting of coal and rock waste is used. Shaft sinking rate ranges from 10 to 50 m/month. The following hoisting schemes are analyzed: two independent systems which consist of a cage with counterweight, three systems of a cage with counterweight, double cage system and a cage with counterweight. Hoisting schemes are shown in 9 diagrams. Investigations show that a 7 to 8 m diameter of mine shafts is most economic. In mine shafts 7 m in diameter equipped with two cages with counterweights one of the cages is removed to form a free space for the hoisting bucket. In the 8 m shaft equipped with a double cage system and a cage with counterweight the cage with counterweight is removed to form a free place for the hoisting bucket used during shaft excavation.

ESF [Exploratory Shaft Facility] impact evaluation report: Volume 1, Final report

International Nuclear Information System (INIS)

1985-08-01

This report assesses the impacts of integrating an Exploratory Shaft Facility (ESF) with a high-level nuclear waste repository in salt. A general repository subsurface design is described which complies with the Mine Safety and Health Administration regulations for gassy metal and non-metal mines. This design is combined with the ESF into a site-specific subsurface layout with associated shafts and surface facilities for each of seven sites. An evaluation to identify integration impacts is described for two specific ESF configurations (Cases 1 and 2) for each of the seven sites. These configurations are an ESF which uses two of the full size repository shafts, and an ESF with one 10-ft and one 22-ft diameter shaft. An evaluation of an ESF configuration (Case 3) with two 12-ft diameter shafts at three of the seven sites is also described. These sites are Deaf Smith, Davis Canyon, and Richton Dome. A fourth evaluation (Case 4) for the Deaf Smith site only, addresses a ''fast track'' subsurface development plan to allow waste emplacement by 1998. A fifth evaluation (Case 5), provides site-specific ES locations, for the three sites included in Case 3, which are supportive of a shaft siting study prepared by ONWI

Waste Handling Shaft concrete liner degradation conclusions and recommendations

International Nuclear Information System (INIS)

1992-10-01

The primary function of the Waste Handling Shaft (WHS) at the Waste Isolation Pilot Plant (WIPP) is to permit the transfer of radioactive waste from the surface waste handling building to the underground storage area. It also serves as an intake shaft for small volumes of air during normal storage operations and as an emergency escape route. Part of the construction was the placement of a concrete liner and steel reinforced key in 1984. During a routine shaft inspection in May 1990, some degradation of the WHS concrete liner was observed between the depths of 800 and 900 feet below the ground surface. Detailed investigations of the liner had been carried out by Sandia National Laboratories and by Westinghouse Electric Corporation Waste Isolation Division (WID) through Lankard Materials Laboratory. Observations, reports, and data support the conclusion that the concrete degradation, resulting from attack by chemically aggressive brine, is a localized phenomena. It is the opinion of the WID that the degradation is not considered an immediate or near term concern; this is supported by technical experts. WID recommendations have been made which, when implemented, will ensure an extended liner life. Based on the current assessment of available data and the proposed shaft liner monitoring program described in this report, it is reasonable to assume that the operational life of the concrete shaft liner can safely support the 25-year life of the WIPP. Analysis of data indicates that degradation of the shaft's concrete liner is attributed to chemically aggressive brine seeping through construction joints and shrinkage cracks from behind the liner in and around the 834-foot depth. Chemical and mechanical components of concrete degradation have been identified. Chemical attack is comprised of several stages of concrete alteration. The other component, mechanical degradation, results from the expansive forces of crystals forming in the concrete pore space

Lichen planus following tetanus-diphtheria-acellular pertussis vaccination: A case report and review of the literature.

Science.gov (United States)

Rosengard, Heather C; Wheat, Chikoti M; Tilson, Matthew P; Cuda, Jonathan D

2018-01-01

Lichen planus is an inflammatory dermatosis with a prevalence of approximately 1%. Recent meta-analyses show that patients with hepatitis C virus have a 2.5- to 4.5-fold increased risk of developing lichen planus. Lichen planus has also followed vaccinations and has specifically been attributed to the hepatitis B vaccine, the influenza vaccine, and the tetanus-diphtheria-acellular pertussis vaccine. We describe a case of lichen planus in a hepatitis C virus-infected African American male occurring in temporal association with the administration of the tetanus-diphtheria-acellular pertussis vaccine. The patient's presentation was clinically consistent with lichen planus and confirmed by biopsy. It is likely that many cases of vaccine-induced lichen planus have gone unpublished or unrecognized. In areas with high prevalence of hepatitis C virus infection, we may expect to see more cases of vaccine-induced lichen planus especially in light of the updated Centers for Disease Control and Prevention tetanus-diphtheria-acellular pertussis vaccination recommendations. This case serves to educate healthcare providers about vaccine-induced lichen planus and, in particular, the need to counsel hepatitis C virus-infected patients about a potential risk of developing lichen planus following vaccination. We also reflect on current theories suggesting the T-cell-mediated pathogenesis of lichen planus and the role that hepatitis C virus and toxoid or protein vaccines may play in initiating the disease.

Structural basis of typhod: Salmonella typhi type IVb pilin (PilS) and cystic fibrosis transmembrane conductance regulator interaction

Energy Technology Data Exchange (ETDEWEB)

Balakrishna, A.; Saxena, A; Mok, H; Swaminathan, K

2009-01-01

The type IVb pilus of the enteropathogenic bacteria Salmonella typhi is a major adhesion factor during the entry of this pathogen into gastrointestinal epithelial cells. Its target of adhesion is a stretch of 10 residues from the first extracellular domain of cystic fibrosis transmembrane conductance regulator (CFTR). The crystal structure of the N-terminal 25 amino acid deleted S. typhi native PilS protein (PilS), which makes the pilus, was determined at 1.9 A resolution by the multiwavelength anomalous dispersion method. Also, the structure of the complex of PilS and a target CFTR peptide, determined at 1.8 A, confirms that residues 113-117 (NKEER) of CFTR are involved in binding with the pilin protein and gives us insight on the amino acids that are essential for binding. Furthermore, we have also explored the role of a conserved disulfide bridge in pilus formation. The subunit structure and assembly architecture are crucial for understanding pilus functions and designing suitable therapeutics against typhoid.

Structural basis of typhoid: Salmonella typhi type IVb pilin (PiLS) and cystic fibrosis transmembrane conductance regulator interaction

Energy Technology Data Exchange (ETDEWEB)

Balakrishna, A.M.; Saxena, A.; Mok, H. Y.-K.; Swaminathan, K.

2009-11-01

The type IVb pilus of the enteropathogenic bacteria Salmonella typhi is a major adhesion factor during the entry of this pathogen into gastrointestinal epithelial cells. Its target of adhesion is a stretch of 10 residues from the first extracellular domain of cystic fibrosis transmembrane conductance regulator (CFTR). The crystal structure of the N-terminal 25 amino acid deleted S. typhi native PilS protein ({Delta}PilS), which makes the pilus, was determined at 1.9 {angstrom} resolution by the multiwavelength anomalous dispersion method. Also, the structure of the complex of {Delta}PilS and a target CFTR peptide, determined at 1.8 {angstrom}, confirms that residues 113-117 (NKEER) of CFTR are involved in binding with the pilin protein and gives us insight on the amino acids that are essential for binding. Furthermore, we have also explored the role of a conserved disulfide bridge in pilus formation. The subunit structure and assembly architecture are crucial for understanding pilus functions and designing suitable therapeutics against typhoid.

Structural Basis of Typhoid: Salmonella typhi Type IVb pilin (PilS) and Cystic Fibrosis Transmembrane Conductance Regulatory Interaction

Energy Technology Data Exchange (ETDEWEB)

Balakrishna, A.; Saxena, A; Mok, H; Swaminathan, K

2009-01-01

The type IVb pilus of the enteropathogenic bacteria Salmonella typhi is a major adhesion factor during the entry of this pathogen into gastrointestinal epithelial cells. Its target of adhesion is a stretch of 10 residues from the first extracellular domain of cystic fibrosis transmembrane conductance regulator (CFTR). The crystal structure of the N-terminal 25 amino acid deleted S. typhi native PilS protein (PilS), which makes the pilus, was determined at 1.9 A resolution by the multiwavelength anomalous dispersion method. Also, the structure of the complex of PilS and a target CFTR peptide, determined at 1.8 A, confirms that residues 113-117 (NKEER) of CFTR are involved in binding with the pilin protein and gives us insight on the amino acids that are essential for binding. Furthermore, we have also explored the role of a conserved disulfide bridge in pilus formation. The subunit structure and assembly architecture are crucial for understanding pilus functions and designing suitable therapeutics against typhoid.

Electromagnetic shaft seal

International Nuclear Information System (INIS)

Takahashi, Kenji.

1994-01-01

As an electromagnetic shaft seal, there are disposed outwarding electromagnetic induction devices having generating power directing to an electroconductive fluid as an object of sealing, and inwarding electromagnetic induction device added coaxially. There are disposed elongate rectangular looped first coils having a predetermined inner diameter, second coils having the same shape and shifted by a predetermined pitch relative to the first coil and third coil having the same shape and shifted by a predetermined pitch relative to the second coil respectively each at a predetermined inner diameter of clearance to the outwarding electromagnetic induction devices and the inwarding electromagnetic induction device. If the inwarding electromagnetic induction device and the outwarding electromagnetic induction device are operated, they are stopped at a point that the generating power of the former is equal with the sum of the generating power of the latter and a differential pressure. When three-phase AC is charged to the first coil, the second coil and the third coil successively, a force is generated in the advancing direction of the magnetic field in the electroconductive fluid by the similar effect to that of a linear motor, and the seal is maintained at high reliability. Moreover, the limit for the rotational angle of the shaft is not caused. (N.H.)

Residual stress analysis of drive shafts after induction hardening

Energy Technology Data Exchange (ETDEWEB)

Lemos, Guilherme Vieira Braga; Rocha, Alexandre da Silva; Nunes, Rafael Menezes, E-mail: lemos_gl@yahoo.com.br [Universidade Federal do Rio Grande do Sul (UFRS), Porto Algre, RS (Brazil); Hirsch, Thomas Karl [Stiftung Institut für Werkstofftechnik (IWT), Bremen (Germany)

2014-08-15

Typically, for automotive shafts, shape distortion manifests itself in most cases after the induction hardening by an effect known as bending. The distortion results in a boost of costs, especially due to machining parts in the hardened state to fabricate its final tolerances. In the present study, residual stress measurements were carried out on automotive drive shafts made of DIN 38B3 steel. The samples were selected in consequence of their different distortion properties by an industrial manufacturing line. One tested shaft was straightened, because of the considerable dimensional variation and the other one not. Firstly, the residual stress measurements were carried out by using a portable diffractometer, in order to avoid cutting the shafts and evaluate the original state of the stresses, and afterwards a more detailed analysis was realized by a conventional stationary diffractometer. The obtained results presented an overview of the surface residual stress profiles after induction hardening and displayed the influence of the straightening process on the redistribution of residual stresses. They also indicated that the effects of the straightening in the residual stresses cannot be neglected. (author)

Interaction between clay-based shaft seal components and crystalline host rock

International Nuclear Information System (INIS)

Priyanto, D.; Dixon, D.; Man, A.

2010-01-01

Document available in extended abstract form only. The Government of Canada has accepted the Nuclear Waste Management Organization's (NWMO) recommendation of Adaptive Phased Management (APM) as the long-term management approach for Canada's used nuclear fuel. APM ultimately involves the isolation and containment of used nuclear fuel deep in a Deep Geological Repository (DGR). On completion of waste emplacement operation and during repository closure, shaft seals, comprising clay-based shaft seal components, will be installed at strategic locations, such as where significant fracture zones (FZs) are located. The primary function of a shaft seal is to limit and prevent short-circuiting of the groundwater flow regime via the shaft. Currently, at Atomic Energy of Canada Limited's Underground Research Laboratory (URL) a full-scale shaft seal is being constructed at the intersection of a low dipping thrust fault called FZ 2 as part of the overall URL decommissioning activities. Both crystalline rock and sedimentary rock are considered potentially suitable host rocks formations for a DGR. This paper presents the results of numerical simulation of a shaft seal installed in moderately to sparsely fractured crystalline rock (MFR). The shape and thickness of the shaft seal modelled for a DGR in this exercise are similar to the shaft seal at the URL, but in the modelling exercise it is given a larger diameter (i.e. 7.30 m) equal to the assumed diameter of a production shaft of a repository. The seal consists of a blended bentonite-sand (BS) component that is constrained between two massive concrete seals. Dense backfill (DBF) materials are installed above and below the concrete seals (CS). The concrete seals are keyed into the access shaft to better anchor the concrete units in place and in order to restrain the swelling of the bentonite-sand component of the seal as it hydrates. The reference geosphere in the proposed work is MFR similar to the rock conditions

Channels Formed by Botulinum, Tetanus, and Diphtheria Toxins in Planar Lipid Bilayers: Relevance to Translocation of Proteins across Membranes

Science.gov (United States)

Hoch, David H.; Romero-Mira, Miryam; Ehrlich, Barbara E.; Finkelstein, Alan; Dasgupta, Bibhuti R.; Simpson, Lance L.

1985-03-01

The heavy chains of both botulinum neurotoxin type B and tetanus toxin form channels in planar bilayer membranes. These channels have pH-dependent and voltage-dependent properties that are remarkably similar to those previously described for diphtheria toxin. Selectivity experiments with anions and cations show that the channels formed by the heavy chains of all three toxins are large; thus, these channels could serve as ``tunnel proteins'' for translocation of active peptide fragments. These findings support the hypothesis that the active fragments of botulinum neurotoxin and tetanus toxin, like that of diphtheria toxin, are translocated across the membranes of acidic vesicles.

Advisibility of excavating an additional central shaft to reduce duration of mine construction. [USSR

Energy Technology Data Exchange (ETDEWEB)

Durov, E.M.

1981-09-01

This paper evaluates methods of reducing duration and cost of underground black coal mine construction. A proposed scheme of coal deposit opening by a system of three mine shafts is critically analyzed. In comparison to the conventional scheme, the proposed one has one additional mine shaft in the central mine zone. The shaft, equipped with a cage hoisting system, permits the rate of mine drivage in the center of the mine to be increased. The cage hoisting system in the additional shaft is activated before the main skip shaft has been equipped and is in operation. Duration of mine construction is reduced by 6 to 10 months. Exacavation of the additional shaft costs from 2.5 to 3.5 million rubles. A further system of mine construction is also analyzed. The main shaft in the mine center is temporarily equipped with a cage hoisting system. The cage system is used for hoisting rocks removed from mine headings and main workings constructed at the bottom of the main shaft. The main shaft is equipped with skips and hoisting tower at a later stage when construction of main roadways and structures at its bottom has been completed. The proposed system permits mine construction to be reduced by 4 to 5 months without a major increase in investment.

Failure analysis of axle shaft of a fork lift

Directory of Open Access Journals (Sweden)

Souvik Das

2015-04-01

Full Text Available An axle shaft of fork lift failed at operation within 296 h of service. The shaft transmits torque from discrepancy to wheel through planetary gear arrangement. A section of fractured axle shaft made of induction-hardened steel was analyzed to determine the root cause of the failure. Optical microscopies as well as field emission gun scanning electron microscopy (FEG-SEM along with energy dispersive spectroscopy (EDS were carried out to characterize the microstructure. Hardness profile throughout the cross-section was evaluated by micro-hardness measurements. Chemical analysis indicated that the shaft was made of 42CrMo4 steel grade as per specification. Microstructural analysis and micro-hardness profile revealed that the shaft was improperly heat treated resulting in a brittle case, where crack was found to initiate from the case in a brittle mode in contrast to ductile mode within the core. This behaviour was related to differences in microstructure, which was observed to be martensitic within the case with a micro-hardness equivalent to 735 HV, and a mixture of non-homogeneous structure of pearlite and ferrite within the core with a hardness of 210 HV. The analysis suggests that the fracture initiated from the martensitic case as brittle mode due to improper heat treatment process (high hardness. Moreover the inclusions along the hot working direction i.e. in the longitudinal axis made the component more susceptible to failure.

ANALYTICAL EVALUATION OF CRACK PROPAGATION FOR BULB HYDRAULIC TURBINES SHAFTS

Directory of Open Access Journals (Sweden)

Mircea O. POPOVICU

2011-05-01

Full Text Available The Hydroelectric Power Plants uses the regenerating energy of rivers. The hydraulic Bulb turbines running with low heads are excellent alternative energy sources. The shafts of these units present themselves as massive pieces, with cylindrical shape, manufactured from low-alloyed steels. The paper analyses the fatigue cracks occurring at some turbines in the neighbourhood of the connection zone between the shaft and the turbine runner flange. To obtain the tension state in this zone ANSIS and AFGROW computing programs were used. The number of running hours until the piercing of the shaft wall is established as a useful result.

Study on collapse mechanism of junction between greatly deeper shaft and horizontal drifts (Contract research)

International Nuclear Information System (INIS)

Kurosaki, Yukio; Yamachi, Hiroshi; Katsunuma, Yoshio; Nakata, Masao; Kuwahara, Hideki; Yamada, Fumitaka; Matsushita, Kiyoshi; Sato, Toshinori

2008-03-01

The Mizunami underground research laboratory is planned to consist of greatly deeper shaft and horizontal drifts. A junction space between a greatly deeper shaft and horizontal drifts forms which would take a complicated mechanical behavior during a junction excavation. However, a quantitative design method of supporting measures for a deep junction has not yet been established. This is because a conventional shaft design has been conducted based on past experience. Detail records have not been left either in what kind of collapses and deformed phenomena occurring in shaft constructions in a past. In order to examine a collapse mechanism of greatly deeper shaft junction, we have conducted literature surveys and interview studies concerned with deep shaft construction works in a past, and investigated what collapses or difficulties had been occurred in deep shaft junctions. Considering the results of investigations with reviews of intellectuals, a collapse mechanism of a super deep shaft junction depends on both a construction procedure of shaft junction and a geological condition at great depth. During a construction of a shaft junction, stress state of rock masses near junction wall would take a complicated stress path. Especially, it should be necessary to take a most careful consideration on that tangential stress acted around a shaft wall may reduce during horizontal drift excavation. On the other hand, where greatly deeper junction intersects faults and/or fractures with a large angle, a collapse called 'Take-nuke' may occur or extraordinary earth pressure acts on a concrete wall. This is the most typical difficulties during shaft construction. In order to recognize a mechanism of these phenomena and to find out a cause of collapse generation, numerical studies that can simulate a practical rock mass behavior around a shaft junction should be carry out. We demonstrate the finite difference method is most adequate for these simulations with intellectual review

Numerically Analysed Thermal Condition of Hearth Rollers with the Water-Cooled Shaft

Directory of Open Access Journals (Sweden)

A. V. Ivanov

2016-01-01

Full Text Available Continuous furnaces with roller hearth have wide application in the steel industry. Typically, furnaces with roller hearth belong to the class of medium-temperature heat treatment furnaces, but can be used to heat the billets for rolling. In this case, the furnaces belong to the class of high temperature heating furnaces, and their efficiency depends significantly on the reliability of the roller hearth furnace. In the high temperature heating furnaces are used three types of watercooled shaft rollers, namely rollers without insulation, rollers with insulating screens placed between the barrel and the shaft, and rollers with bulk insulation. The definition of the operating conditions of rollers with water-cooled shaft greatly facilitates the choice of their design parameters when designing. In this regard, at the design stage of the furnace with roller hearth, it is important to have information about the temperature distribution in the body of the rollers at various operating conditions. The article presents the research results of the temperature field of the hearth rollers of metallurgical heating furnaces. Modeling of stationary heat exchange between the oven atmosphere and a surface of rollers, and between the cooling water and shaft was executed by finite elements method. Temperature fields in the water-cooled shaft rollers of various designs are explored. The water-cooled shaft rollers without isolation, rollers with screen and rollers with bulk insulation, placed between the barrel and the water-cooled shaft were investigated. Determined the change of the thermo-physic parameters of the coolant, the temperature change of water when flowing in a pipe and shaft, as well as the desired pressure to supply water with a specified flow rate. Heat transfer coefficients between the cooling water and the shaft were determined directly during the solution based on the specified boundary conditions. Found that the greatest heat losses occur in the

Shaft extension design at the Underground Research Laboratory, Pinawa, Manitoba

International Nuclear Information System (INIS)

Kuzyk, G.W.; Ball, A.E.

1991-01-01

AECL Research has constructed an underground laboratory for the research and development required for the Canadian Nuclear Fuel Waste Management Program. The experimental program in the laboratory will contribute to the assessment of the feasibility and safety of nuclear fuel waste disposal deep in stable plutonic rock. In 1988, AECL extended the shaft of the Underground Research Laboratory (URL) from the existing 255 m depth to a depth of 443 m in cooperation with the United States Department of Energy. The project, which involved carrying out research activities while excavation and construction work was in progress, required careful planning. To accommodate the research programs, full-face blasting with a burn cut was used to advance the shaft. Existing facilities at the URL had to be modified to accommodate an expanded underground facility at a new depth. This paper discusses the design criteria, shaft-sinking methods and approaches used to accommodate the research work during this shaft extension project. (11 refs., 11 figs.)

Research and industrialization of near-net rolling technology used in shaft parts

Science.gov (United States)

Hu, Zhenghuan; Wang, Baoyu; Zheng, Zhenhua

2018-03-01

Shaft part rolling is an efficient and green nearnet shaping technology offering many advantages, including high production efficiency, high material utilization rate, high product quality, and excellent production environment. In this paper, the features of shaft part rolling are introduced along with the working principles of two main shaft part rolling technologies, namely, cross wedge rolling (CWR) and skew rolling (SR). In relation to this technology, some R&D achievements gained by the University of Science and Technology Beijing are summarized. Finally, the latest developments in shaft part rolling are presented, including SR steel balls, precise forming of camshaft blank by CWR, SR phosphorous copper balls at room temperature, and CWR hollow axle sleeve. Although the shaft part rolling technology has been widely used in China, it only accounts for about 15% of applicable parts at present. Nevertheless, this technology has broad application prospects.

Fatigue criterion for the design of rotating shafts under combined stress

Science.gov (United States)

Loewenthal, S. H.

1977-01-01

A revised approach to the design of transmission shafting which considers the flexure fatigue characteristics of the shaft material under combined cyclic bending and static torsion stress is presented. A fatigue failure relation, corroborated by published combined stress test data, is presented which shows an elliptical variation of reversed bending endurance strength with static torsional stress. From this elliptical failure relations, a design formula for computing the diameter of rotating solid shafts under the most common condition of loading is developed.

Design and Analysis of Drive Shaft using Kevlar/Epoxy and Glass/Epoxy as a Composite Material

Science.gov (United States)

Karthikeyan, P.; Gobinath, R.; Kumar, L. Ajith; Jenish, D. Xavier

2017-05-01

In automobile industry drive shaft is one of the most important components to transmit power form the engine to rear wheel through the differential gear. Generally steel drive shaft is used in automobile industry, nowadays they are more interested to replace steel drive shaft with that of composite drive shaft. The overall objective of this paper is to analyze the composite drive shaft using to find out the best replacement for conventional steel drive shaft. The uses of advanced composite materials such as Kevlar, Graphite, Carbon and Glass with proper resins ware resulted in remarkable achievements in automobile industry because of its greater specific strength and specific modulus, improved fatigue and corrosion resistances and reduction in energy requirements due to reduction in weight as compared to steel shaft. This paper is to presents, the modeling and analysis of drive shaft using Kevlar/Epoxy and Glass/Epoxy as a composite material and to find best replacement for conventional steel drive shafts with an Kevlar/epoxy or Glass/Epoxy resin composite drive shaft. Modeling is done using CATIA software and Analysis is carried out by using ANSYS 10.0 software for easy understanding. The composite drive shaft reduces the weight by 81.67 % for Kevlar/Epoxy and 72.66% for Glass/Epoxy when compared with conventional steel drive shaft.

Research on dynamic balancing simulation of rotary shaft based on ADAMS

Science.gov (United States)

Zheng, Weiqiang; Rui, Chengjie; Yang, Jie; Liu, Pingyi

2018-02-01

Due to the design and processing technology of rotary shaft, the mass center of it does not coincide with the rotating axis of the rotary shaft and there is an unbalanced mass. The unbalanced mass can have some disadvantages, such as the centrifugal force, the vibration and so on. Those disadvantages could reduce the accuracy and service life of the equipment.In this paper, the dynamic balance of the rotary shaft is analysed by the theory analysis combined with the dynamic simulation software. This method ensures that the rotary shaft meets the dynamic balancing requirements during the design stage. It effectively supports the structural design of the rotary shift, and provides a way of thinking and method for the design and development of the same type of products.

METHOD OF ACHIEVING ACCURACY OF THERMO-MECHANICAL TREATMENT OF LOW-RIGIDITY SHAFTS

Directory of Open Access Journals (Sweden)

Antoni Świć

2016-03-01

Full Text Available The paper presents a method combining the processes of straightening and thermal treatment. Technological processes with axial strain were considered, for the case of heated material and without its heating. The essence of the process in the case of heated material consisted in the fact that if under tension all longitudinal forces in the first approximation are uniform - the same strains are generated. The presented technological approach, aimed at reducing the curvature of axial-symmetrical parts, is acceptable as the process of rough, preliminary machining, in the case of shafts with the ratio L/D≤100 (L – shaft length, d – shaft diameter and without a tendency of strengthening. To improve the accuracy and stability of geometric form of low-rigidity parts, a method was developed that combines the processes of straightening and heat treatment. The method consists in that axial strain – tension, is applied to the shaft during heating, and during cooling the product is fixed in a fixture, the cooling rate of the shaft being several-fold greater than that of the fixture. A device is presented for the realisation of the method of controlling the process of plastic deformation of low-rigidity shafts. In the case of the presented device and the adopted calculation scheme, a method was developed that permits the determination of the length of shaft section and of the time of its cooling.

Development Of Design Equations For A Square-tube Subbase Supporting A Shaft-mounted Speed Reducer

OpenAIRE

Brown III, William E.

2002-01-01

Shaft mounted speed reducers are used in material handling applications, such as conveyor systems for transporting ore out of mine shafts. A subbase joins the reducer with an electric motor, and serves to limit the misalignment between the motor shaft and the reducer input shaft. The entire assembly is supported at two points: the axis of rotation of the reducer output shaft, which is fixed, and a clevis-pin joint under the motor, which prevents rotation of the assembly about the reducer ou...

Mechanical coupling for a rotor shaft assembly of dissimilar materials

Science.gov (United States)

Shi, Jun [Glastonbury, CT; Bombara, David [New Hartford, CT; Green, Kevin E [Broad Brook, CT; Bird, Connic [Rocky Hill, CT; Holowczak, John [South Windsor, CT

2009-05-05

A mechanical coupling for coupling a ceramic disc member to a metallic shaft includes a first wedge clamp and a second wedge clamp. A fastener engages a threaded end of a tie-bolt to sandwich the ceramic disc between the wedge clamps. An axial spring is positioned between the fastener and the second wedge clamp to apply an axial preload along the longitudinal axis. Another coupling utilizes a rotor shaft end of a metallic rotor shaft as one wedge clamp. Still another coupling includes a solid ceramic rotor disc with a multiple of tie-bolts radially displaced from the longitudinal axis to exert the preload on the solid ceramic rotor disc.

Design of inclined loaded drilled shafts in high-plasticity clay environment.

Science.gov (United States)

2011-05-01

Drilled shaft foundations are principally used to support many structures such as bridge piers, towers, : buildings, transmission towers, and roadway cable barriers. This research focuses on the use of drilled shafts : in the cable median barrier sys...

Shaft Excavation in Frozen Ground at Point 5

CERN Document Server

Osborne, J

2000-01-01

Construction work on the 112 MCHF civil engineering contract started at Point 5 in August 1998. The new surface buildings and underground structures are necessary to accommodate the CMS detector for the LHC Project. The principal underground works consist of two new shafts, two parallel caverns separated by a supporting pillar, and a number of small connection tunnels and service galleries. The two shafts are to be sunk through approximately 50 m of water-bearing moraine to the underlying molasse rock. From a number of possible construction methods, ground freezing of the moraine was considered to be most appropriate. The ground freezing is used to control the groundwater and to support temporarily the moraine during excavation and lining of the shafts. The aim of this paper is to present the ground-freezing technique and to discuss the advantages and disadvantages of the system in the light of its first few months of running on the Point 5 site.

Fracture Failure Analysis of Fuel Pump Transmission Shaft of Dual-Fuel Engine

Directory of Open Access Journals (Sweden)

Chen Pei-hong

2017-01-01

Full Text Available NTS6ZLCz-129 dual-fuel turbocharged and intercooled engine durability test at 1000h, fuel pump shaft fractured. Fracture analysis, chemical analysis, microstructure examination and finite element stress analysis were carried out on the fractured shaft. The analysis result showed that the shaft fracture cause is forging fold. By improving the forging process, the forging fold was solved, and the durability test can be carried out smoothly.

33 Shafts Category of Transuranic Waste Stored Below Ground within Area G

Energy Technology Data Exchange (ETDEWEB)

Hargis, Kenneth Marshall [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Monk, Thomas H [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2017-05-22

This report compiles information to support the evaluation of alternatives and analysis of regulatory paths forward for the 33 shafts. The historical information includes a form completed by waste generators for each waste package (Reference 6) that included a waste description, estimates of Pu-239 and uranium-235 (U-235) based on an accounting technique, and calculations of mixed fission products (MFP) based on radiation measurements. A 1979 letter and questionnaire (Reference 7) provides information on waste packaging of hot cell waste and the configuration of disposal shafts as storage in the 33 Shafts was initiated. Tables of data by waste package were developed during a review of historical documents that was performed in 2005 (Reference 8). Radiological data was coupled with material-type data to estimate the initial isotopic content of each waste package and an Oak Ridge National Laboratory computer code was used to calculate 2009 decay levels. Other sources of information include a waste disposal logbook for the 33 shafts (Reference 9), reports that summarize remote-handled waste generated at the CMR facility (Reference 10) and placement of waste in the 33 shafts (Reference 11), a report on decommissioning of the LAMPRE reactor (Reference 12), interviews with an employee and manager involved in placing waste in the 33 shafts (References 13 and 14), an interview with a long-time LANL employee involved in waste operations (Reference 15), a 2002 plan for disposition of remote-handled TRU waste (Reference 16), and photographs obtained during field surveys of several shafts in 2007. The WIPP Central Characterization Project (CCP) completed an Acceptable Knowledge (AK) summary report for 16 canisters of remote-handled waste from the CMR Facility that contains information relevant to the 33 Shafts on hot-cell operations and timeline (Reference 17).

ESF [Exploratory Shaft Facility] impact evaluation report: Volume 2: Final report

International Nuclear Information System (INIS)

1985-08-01

This report assesses the impacts of integrating an Exploratory Shaft Facility (ESF) with a high-level nuclear waste repository in salt. An evaluation to identify integration impacts is described for two specific ESF configurations (Cases 1 and 2) for each of the seven sites. These configurations are an ESF which uses two of the full size repository shafts, and an ESF with one 10-ft and one 22-ft diameter shaft. An evaluation of an ESF configuration (Case 3) with two 12-ft diameter shafts at three of the seven sites is also described. These sites are Deaf Smith, Davis Canyon, and Richton Dome. A fourth evaluation (Case 4) for the Deaf Smith site only, addresses a ''fast track'' subsurface development plan to allow waste emplacement by 1998. A fifth evaluation (Case 5), provides site-specific ES locations, for the three sites included in Case 3, which are supportive of a shaft siting study prepared by ONWI. The report presents development schedules depicting construction activities and time frames commencing with receipt of the repository Construction Authorization and proceeding to initiation of emplacement operations. These schedules are site specific and are presented for each of the five cases

Low frequency torsional vibration gaps in the shaft with locally resonant structures

International Nuclear Information System (INIS)

Yu Dianlong; Liu Yaozong; Wang Gang; Cai Li; Qiu Jing

2006-01-01

The propagation of torsional wave in the shaft with periodically attached local resonators is studied with the transfer matrix theory and the finite element method. The analytical dispersion relation and the complex band structure of such a structure is presented for the first time, which indicates the existence of low frequency gaps. The effect of shaft material on the vibration attenuation in band gap is investigated. The frequency response function of the shaft with finite periodic locally resonant oscillators is simulated with finite element method, which shows large vibration attenuation in the frequency range of the gap as expected. The low frequency torsional gap in shafts provides a new idea for vibration control

The effect of prophylaxis with chloroquine and proguanil on delayed-type hypersensitivity and antibody production following vaccination with diphtheria, tetanus, polio, and pneumococcal vaccines

DEFF Research Database (Denmark)

Gyhrs, A; Pedersen, B K; Bygbjerg, I

1991-01-01

(1,000 mg/week), or 4) proguanil hydrochloride (200 mg/day) for six weeks. Skin testing was performed on days 0 and 28. Vaccinations with diphtheria, tetanus, polio, and pneumococcal polysaccharide antigen vaccines were performed on day 28, and the presence of specific antibodies was determined...... dosages, does not induce any detectable suppression of delayed-type hypersensitivity or vaccination responses to diphtheria, tetanus, polio, or pneumococcal polysaccharide antigens....

Heat generation and hemolysis at the shaft seal in centrifugal blood pumps.

Science.gov (United States)

Araki, K; Taenaka, Y; Wakisaka, Y; Masuzawa, T; Tatsumi, E; Nakatani, T; Baba, Y; Yagura, A; Eya, K; Toda, K

1995-01-01

The heat and hemolysis around a shaft seal were investigated. Materials were original pumps (Nikkiso HMS-15:N-original, and 3M Delphin:D-original), vane-removed pumps (Nvane(-), Dvane(-)), and a small chamber with a shaft coiled by nichrome wire (mock pump). The original pumps were driven at 500 mmHg and 5 L/min, and vane-removed pumps were driven at the same rotation number. An electrical powers of 0, 0.5, 2, and 10 W was supplied to the mock pumps. In vitro hemolytic testing showed that hemolytic indices were 0.027 g/100 L in N-original, 0.013 in Nvane(-), 0.061 in D-original, and 0.012 in Dvane(-). Measurement of heat with a thermally insulated water chamber showed total heat within the pump of 8.62 and 10.85 W, and heat at the shaft seal of 0.87 and 0.62 W in the Nikkiso and Delphin pumps, respectively. Hemolysis and heat generation of mock pumps remained low. The results indicate that the heat generated around the shaft seal was minimal. Hemolysis at the shaft-seal was considerable but not major. Local heat did not affect hemolysis. It was concluded that the shaft-seal affected hemolysis, not by local heat but friction itself.

Long-term functional outcome following intramedullary nailing of femoral shaft fractures

NARCIS (Netherlands)

el Moumni, Mostafa; Voogd, Emma Heather; ten Duis, Henk Jan; Wendt, Klaus Wilhelm

Background: The management of femoral shaft fractures using intramedullary nailing is a popular method. The purpose of this study was to evaluate the long-term functional outcome after antegrade or retrograde intramedullary nailing of traumatic femoral shaft fractures. We further determined

Gearbox Reliability Collaborative Investigation of Gearbox Motion and High-Speed-Shaft Loads

Energy Technology Data Exchange (ETDEWEB)

Keller, Jon [National Renewable Energy Lab. (NREL), Golden, CO (United States); Guo, Yi [National Renewable Energy Lab. (NREL), Golden, CO (United States); Sethuraman, Latha [National Renewable Energy Lab. (NREL), Golden, CO (United States)

2016-03-18

This paper extends a model-to-test validation effort to examine the effect of different constant rotor torque and moment conditions and intentional generator misalignment on the gearbox motion and high-speed-shaft loads. Fully validating gearbox motion and high-speed-shaft loads across a range of test conditions is a critical precursor to examining the bearing loads, as the gearbox motion and high-speed-shaft loads are the drivers of these bearing loads.

Carrier state of Haemophilus influenzae type b (Hib, Streptococcus pneumoniae, Streptococcus pyogenes, Neisseria meningitidis and Corynebacterium diphtheriae among school children in Pokhara, Nepal

Directory of Open Access Journals (Sweden)

Dharm Raj Bhatta

2014-02-01

Full Text Available Objective: To determine the incidence of carrier state of Haemophilus influenzae type b, Streptococcus pneumoniae (S. pneumoniae, Streptococcus pyogenes, Neisseria meningitidis and Corynebacterium diphtheriae among school children. Methods: Specimen from posterior pharyngeal wall and tonsils were collected on calcium alginate coated swabs from 1 02 participants. Processing of specimen and antimicrobial susceptibility testing was done by standard procedures. Results: Potential pathogens isolated in our study were S. pneumoniae (14.7%, Staphylococcus aureus (12.7%, Corynebacterium diphtheriae (3.9%, Streptococcus pyogenes (3.9% and Haemophilus influenzae (1.9%. Important findings in antibiogram include high resistance of S. pneumoniae to penicillin (73% and resistance of Staphylococcus aureus to oxacillin (23%. Conclusions: Pharyngeal colonization by S. pneumoniae among school children was found high and there is need of introduction of pneumococcal vaccines among children. Despite expected universal vaccination, pharyngeal colonization by Corynebacterium diphtheriae is possible and there is possibility of transmission.

FINITE ELEMENT ANALYSIS OF CENTRELESS-LUNETTE TURNING OF HEAVY SHAFT

Directory of Open Access Journals (Sweden)

Yu. V. Vasilevich

2017-01-01

Full Text Available Dynamics of huge renovated lathe is simulated. Turning scheme concerns to heavy rotor shaft finishing. Lofty parts and milling head may create dynamic problems. Static, modal and harmonic frequency response function simulations were provided. Bearing system consists of bed, support, tool, lunettes, tailstock. Headstock didn’t take part in shaft holding. Static and dynamic rigidities founded 3–4 times less for support than for shaft. Tool rigidity lessens from 186.5 to 11.9 N/µm for speeding from slow to near resonance turning. Twelve lathe eigenmodes were evaluated. Two eigenmodes are most dangerous. It is “shaft swinging on lunettes” (M1, 26.7 Hz and “support pecking” (M3, 54.4 Hz. Bed has excessive flexibility due to through holes and lack of inner ribbing. Polymer concrete filling is moderately effective. Changing two-lunette (2L scheme to three-lunette (3L increases rigidity of shaft at 2.09 times at statics but gives limited action in dynamics. Resonant peaks on frequency response function are lowered only at 1.32 times for M1, M3. Effect of dynamic damping is revealed under condition of proximity middle lunette to lofty support. Support serves as tuned mass damper. Measures of machine tool reinforcement are simulated. Shaft swinging according to M1 may hardly be blocked by passive means. It would be better to bypass it. “Support pecking” resonance (M3 succumbs to only full set of measures. Small effect of partial reinforcement is predicted. Three frequency intervals are recommended for turn-milling at huge lathe: pre-resonant (<20 Hz, inter-resonant (35–45 Hz and post-resonant (>65 Hz. The last one is more suited. Next design step is to create triangle inner ribbing system or caissons inside of bed.

Tetanus and diphtheria immunity among term and preterm infant-mother pairs in Turkey, a country where maternal and neonatal tetanus have recently been eliminated.

Science.gov (United States)

Erener-Ercan, Tugba; Aslan, Mustafa; Vural, Mehmet; Erginoz, Ethem; Kocazeybek, Bekir; Ercan, Gokmen; Turkgeldi, Lale Wetherilt; Perk, Yildiz

2015-03-01

The aim of our study was to investigate the anti-tetanus and anti-diphtheria antibody titres and the placental transfer of these antibodies in a group of vaccinated and unvaccinated mothers and their term or preterm offsprings. Anti-tetanus and anti-diphtheria toxoid IgG antibodies were measured quantitatively by ELISA in 91 infant-mother pairs. Protective concentrations of anti-tetanus and anti-diphtheria were found in 58.3 and 50% of mothers in the unvaccinated group and 94.5 and 85.5% of the mothers in the vaccinated group. Protective concentrations were found in 63.9 and 50% of cord samples, respectively, in the unvaccinated group and in 96.4 and 85.5% of cord samples, respectively, in the vaccinated group (p = 0.0001). There were no differences in the maternal and cord geometric mean concentrations (GMCs) of anti-toxoid antibodies between those who received two doses or one dose of Td. The GMCs of maternal and cord anti-tetanus and anti-diphtheria were statistically similar between preterm and term groups. Placental transfer ratios (TR) for anti-tetanus and anti-diphtheria were 175 and 150%, respectively, in the preterm group and 213 and 178%, respectively, in the term group. There was a strong correlation between maternal and cord anti-toxoid antibody levels. Maternal vaccination was the only predictor of having protective concentrations of anti-toxoid antibodies in cord blood. Vaccinating pregnant women with at least one dose of Td would confer protection for both the term and preterm infant-mother pairs. Therefore, health personnel caring for pregnant women have the responsibility to emphasize the importance of Td vaccination to avoid missed immunization opportunities.

Evaluation of Immunogenicity of Yersinia enterocolitica O:8 Oligopolysaccaride-DiphtheriaeToxoide Conjugate in Mice

Directory of Open Access Journals (Sweden)

SM Rezavian

2015-06-01

Full Text Available Background & objectives: Yersiniosis is created by Yersinia enterocolitica O:8 and causes problems in the world especialy in cold and mild countries. The purpose of this study is to evaluate the immunogenicity of Yersinia enterocolitica O:8 oligopolysaccaride (OPS conjugate to diphtheria toxoid (DT as a vaccine candidate.   Methods : After cultivation of bacteria, the LPS were isolated by modified hot phenol method. Then dialysis and concentration were done and the OPS were extracted by acetic acid 2%. To conjugate with diphtheria toxoid, ADH was used as a spacer molecule and EDAC as a linker. Conjugate was purified by gel filtration. Then 4 groups of female BALB/c mice were selected (15 mice in each group. Injection was performed intraperitoneally in three doses with two weeks interval. Then serum samples were collected and antibody response against OPS was measured by indirect ELISA method for detection of total IgG, IgA, IgM, IgG1, IgG2a, IgG2b and IgG3.   Results: After second and third doses, OPS-DT recieved group showed significant increase in all types of antibodies titer in anti-OPS in comparison to group that recived nonconjugated OPS. The increase in titer of antibodies was as: OPS-DT>OPS>DT. A remarkable increase was shown in total IgG and IgM titers (with total amount of 3204 and 670, respectively. In IgG1 subclass the amount was 920 and in other subclasses of IgG (IgG3, IgG2a and IgG2b the amounts were 910, 110, and 99, respectively.   Conclusion: The results shows that OPS of Yersinia enterocolitica O:8 increases the anti-OPS antibodies in the form of conjugate with diphtheria toxoid and could be considered as an appropriate vaccine candidate.

Mechanism of Metal Ion Activation of the Diphtheria Toxin Repressor DtxR

Energy Technology Data Exchange (ETDEWEB)

D' Aquino,J.; Tetenbaum-Novatt, J.; White, A.; Berkovitch, F.; Ringe, D.

2005-01-01

The diphtheria toxin repressor (DtxR) is a metal ion-activated transcriptional regulator that has been linked to the virulence of Corynebacterium diphtheriae. Structure determination has shown that there are two metal ion binding sites per repressor monomer, and site-directed mutagenesis has demonstrated that binding site 2 (primary) is essential for recognition of the target DNA repressor, leaving the role of binding site 1 (ancillary) unclear. Calorimetric techniques have demonstrated that although binding site 1 (ancillary) has high affinity for metal ion with a binding constant of 2 x 10{sup -7}, binding site 2 (primary) is a low-affinity binding site with a binding constant of 6.3 x 10{sup -4}. These two binding sites act in an independent fashion, and their contribution can be easily dissected by traditional mutational analysis. Our results clearly demonstrate that binding site 1 (ancillary) is the first one to be occupied during metal ion activation, playing a critical role in stabilization of the repressor. In addition, structural data obtained for the mutants Ni-DtxR(H79A, C102D), reported here, and the previously reported DtxR(H79A) have allowed us to propose a mechanism of metal activation for DtxR.

Simulation on spread of fire smoke in the elevator shaft for a high-rise building

Directory of Open Access Journals (Sweden)

Yunchun Xia

2014-01-01

Full Text Available Spread of fire smoke in the elevator shaft of a high-rise building is influenced by many driving facts. We simulate smoke spreading in the elevator shaft, stair room, and pre-chamber with and without different supplied pressurized air. The simulation shows that smoke moves very fast in the elevator shaft. When a 12 floor high-rise building is in fire, smoke can fill up the elevator shaft in less than 1.5 min after a fire started, temperature in the elevator shaft can be higher than 187°C in 5 min, and the concentration of CO can also reach a high level. The elevator shaft has a very low visibility in less than about 100 s.

Magnetic shaft seals prevent hazardous leakage from wastewater agitators

International Nuclear Information System (INIS)

Traino, F.A.

1985-01-01

The US Department of Energy's laboratory in Miamisburg, OH, operated by Monsanto Research Corporation, processes approximately 45,000 gallons per week of low-level radioactive wastewater to meet Federal Environmental Protection Agency quality standards. Preventing the spread of radioactive contamination throughout the operating area demands effective sealing of all process piping, valves, pumps, and agitators. Rotating shafts of pumps and agitators installed a the start of operations in 1947 were sealed by stuffing glands with graphite impregnated asbestos packing. These pumps proved to be unsatisfactory. In the mid-1970's, new process pumps with mechanical seals and some with magnetic drives were installed. Later, in January 1979, new agitator shaft drives with double tandem, spring-loaded mechanical seals were installed, maintenance of these pumps was costly. The agitator drive shafts were redesigned to accommodate magnetic seals of the type successfully used in blowers and vacuum/pressure pumps in other plant locations. One inherent advantage of the magnetic seal is that it operates with a face loading as much as 50% less than a conventional spring-loaded mechanical seal. The lower loading by a predetermined uniform magnetic force contributes to long face life. Other advantages include compactness, ease of assembly with only a few parts, and insensitivity to vibration. The magnetic shaft seals installed on the agitator shafts in February 1983 are still in service without any leakage or need for maintenance. Based on current operating data and a projected five-year meantime between failures, the estimated cost benefit of the magnetic seals over spring-loaded mechanical seals over spring-loaded mechanical seals will be $640 vs $2400 respectively per seal, with 60% less downtime for maintenance

[A large-scale epidemic of diphtheria in Moscow in recent years: patterns of development].

Science.gov (United States)

Chistiakova, G G; Filatov, N N; Korzhenkova, M P; Solodovnikov, Iu P; Lytkina, I N; Maksimova, N M; Markina, S S

2001-01-01

Data on the dynamics of diphtheria morbidity in Moscow in 1958-1999 are presented. The last epidemic which started at the end of the 1980s and reached its peak in 1994, giving a 59-fold rise in morbidity in comparison with the pre-epidemic period, is characterized in detail. During the epidemic 12,267 persons fell ill, 454 of them died (mortality rate was 4%). Having started in Moscow, the epidemic gradually spread not only over the territory of Russia, but also over some other republics of the former Soviet Union (Ukraine, Belarus, etc.). Possible causes of this epidemic emergency are considered. The ever increasing share of adult population among persons affected by the epidemic (75%) is noted. The infection adults is characterized by severity of clinical manifestations and increased morbidity among adults, is shown. Under complicated social and economic conditions (crisis situation) the increase of groups of high risk which included unemployed adults of working age, retirees as well as socially non-adapted persons, was registered. Mainly these groups determined tense epidemiological situation in diphtheria in Moscow.

Condition monitoring of shaft of single-phase induction motor using optical sensor

Science.gov (United States)

Fulzele, Asmita G.; Arajpure, V. G.; Holay, P. P.; Patil, N. M.

2012-05-01

Transmission type of optical technique is developed to sense the condition of rotating shafts from a distance. A parallel laser beam is passed tangential over the surface of rotating shaft of a single phase induction motor and its flickering shadow is received on a photo sensor. Variations in sensor voltage output are observed on a digital storage oscilloscope. It is demonstrated that this signal carries information about shaft defects like miss alignment, play and impacts in bearings along with surface deformities. Mathematical model of signals corresponding to these shaft defects is developed. During the development and testing of the sensor, effects of reflections are investigated, sensing phenomenon is simulated, frequency response of the sensor is obtained and its performance is compared with conventional accelerometer.

Mining a coal seam with caving in a protective pillar of a mine shaft. [Poland

Energy Technology Data Exchange (ETDEWEB)

Szymura, G; Dilling, R; Kowalski, A

1984-01-01

Mining the 620 seam is evaluated (from 1.5 to 1.7 m thick at a depth of 468 m in the protective pillar of the upcast mine shaft used for ventilation, manriding and transport of materials in the Pstrowski mine in Upper Silesia). The shaft is 496 m deep, has a diameter of 3.5 m and its liners are made of bricks. Ground subsidence caused by underground mining influenced: the head frame above the shaft, residential buildings, a church, railway tracks and a river bed. A system of shortwall mining with caving was used. Deformation of shaft liners was reduced by advanced cutting of a coal block 30x30 m around the shaft. A system of timber cribbings and yielding elements was used. Design of support systems used around the shaft is shown in 3 schemes. Shaft deformation was within permissible limits. The maximum ground subsidence (0.95 m) occurred in the river area. Ground subsidence in the area of the church ranged from 0.75 to 0.81 m and in the head frame area 0.84 m. Accuracy of ground subsidence and shaft deformation forecasting was high. 4 references.

Influence of a reducing agent used to prepare radiopharmaceuticals labeled with technetium-99m on the adhesive properties of Corynebacterium diphtheriae strains

International Nuclear Information System (INIS)

Souza, S.M.; Bernardo-Filho, M.; Hirata, R. Jr.; Moreira, L.O.; Mattos-Guaraldi, A.L.

2002-01-01

Aim: In this work we investigated the influence of stannous chloride (SnCl 2 ) used in nuclear medicine as a reducing agent to prepare radiopharmaceuticals labeled with technetium-99m, on the survival and adhesive properties of two toxigenic C. diphtheriae of the sucrose fermenting (241strain) and non fermenting (CDC-E8392 strain) biotypes. Materials and Methods: Bacterial strains were submitted to survival and filamentation induction assays using different concentrations of SnCl 2 . The influence of SnCl 2 on the adhesive properties of C.diphtheriae were evaluated by bacterial autoaggregation, haemagglutination, adherence to glass surface and lectin-binding assays. Results: Differences in survival fractions suggested differences in susceptibility of microorganisms to bactericidal effect of stannous chloride. A percentage of 0.4% bacterial cells of no.241 strain and 0.04% of CDC-E8392 strain survived after 220 μl ml -1 SnCl 2 treatment. Results of both polystyrene and spontaneous autoaggregation tests showed an increase in the hydrophobic surface properties of C. diphtheriae strains. SnCl 2 induced spontaneous bacterial autoaggregation of sucrose fermenting 241 strain. SnCl 2 enhanced adherence to glass and totally inhibited the haemagglutinating activity of the non-sucrose fermenting strain CDC-E8392 strain (original titer=32). Decrease in haemagglutinatination was dependent on the concentration of SnCl 2 used. Lectin-binding assays demonstrated increase in the expression of cell surface receptors to lectin with affinity for molecules containing mannose residues after treatment with SnCl 2 . The presence of SnCl 2 induced differences in the expression of bacterial surface carbohydrates possibly related with differences in degrees of haemagglutination and adherence to glass of diphtheria bacilli. Conclusion: The presence of SnCl 2 may influence on the adhesive properties of bacterial pathogens. The occurrence of cell filamentation suggests a potential genotoxicity

Diphtheria toxoid-containing microparticulate powder formulations for pulmonary vaccination : Preparation, characterization and evaluation in guinea pigs

NARCIS (Netherlands)

Amidi, Maryam; Pellikaan, Hubert C.; Hirschberg, Hoang; de Boerd, Anne H.; Crommelin, Daan J. A.; Hennink, Wim E.; Kersten, Gideon; Jiskoot, Wim

2007-01-01

this study, the potential of N-Trimethyl chitosan (TMC, degree of quaternization 50%) and dextran microparticles for pulmonary delivery of diphtheria toxoid (DT) was investigated. The antigen-containing microparticles were prepared by drying of an aqueous solution of polymer and DT through a

Analysis and countermeasures for the corrosion on the shaft of seawater pump

International Nuclear Information System (INIS)

Lu Hongtao; Chen Haiming

2010-01-01

The corrosion resistance of the shaft material-3Cr13 was studied through immersion test and electrochemistry test. The results indicated that 3Cr13 and the chromium plating on the shaft had poor resistance against local corrosion in seawater. And the free corrosion potential of 3Cr13 in seawater was lower than other components of the pump, this could accelerate the corrosion rate of the shaft due to galvanic corrosion. A comprehensive analysis showed that the root cause of the corrosion on the No.4 shaft was that 3Cr13 had poor resistance against local corrosion in seawater. Because of the exist of fit-up gap, galvanic corrosion effect and corrosive wear caused by sand, crevice corrosion, galvanic corrosion and wear occurred. All of these accelerated the corrosion rate of the shaft and finally caused its failure. It is suggested that the sealant should be improved and the current material 3Cr13 should be replaced by a kind of materials with better corrosion resistance. (authors)

The Strength Calculation of the Pump Shaft with a Worn Impeller

Directory of Open Access Journals (Sweden)

Nikolay P. Ovchinnikov

2017-12-01

Full Text Available Introduction: This paper presents the study of the impeller wear influence on stress-strain state of a centrifugal pump shaft. In agro-industrial sector, centrifugal pumps are used for watering various agricultural crops. During pumping water, a centrifugal pump impeller is usually a subject to influence of various irreversible physical-and-mechanical and physical-and-chemical processes that can result in a certain reduction in its mass. Materials and Methods: We used a comprehensive approach including the analysis of a sufficient number domestic and foreign publications on the research topic and parametric studies conducted on a laboratory-pumping unit. We had modern vibration-based diagnostic equipment, the mathematical models of loading a pump shaft and a finite-element modeling in APM Win Machine software (Beam module. Results: The comparison of the maximum equivalent dynamic stresses obtained according to the proposed method with existing methods for carrying out the checking strength calculation of a centrifugal pump shaft showed that account of the impeller wear significantly changes picture of stress-strain state shaft. Discussion and Conclusions: The amendments proposed by the author in checking strength calculation of a centrifugal pump shaft will allow estimating its stress-strain state in certain production situations.

Torsional Vibrations of a Conic Shaft with Opposite Tapers Carrying Arbitrary Concentrated Elements

Directory of Open Access Journals (Sweden)

Jia-Jang Wu

2013-01-01

Full Text Available The purpose of this paper is to present the exact solution for free torsional vibrations of a linearly tapered circular shaft carrying a number of concentrated elements. First of all, the equation of motion for free torsional vibration of a conic shaft is transformed into a Bessel equation, and, based on which, the exact displacement function in terms of Bessel functions is obtained. Next, the equations for compatibility of deformations and equilibrium of torsional moments at each attaching point (including the shaft ends between the concentrated elements and the conic shaft with positive and negative tapers are derived. From the last equations, a characteristic equation of the form is obtained. Then, the natural frequencies of the torsional shaft are determined from the determinant equation , and, corresponding to each natural frequency, the column vector for the integration constants, , is obtained from the equation . Substitution of the last integration constants into the associated displacement functions gives the corresponding mode shape of the entire conic shaft. To confirm the reliability of the presented theory, all numerical results obtained from the exact method are compared with those obtained from the conventional finite element method (FEM and good agreement is achieved.

Study of the conditions affecting the critical speed of a rotating pump shaft

International Nuclear Information System (INIS)

Fardeau, P.; Huet, J.L.; Axisa, F.

1983-01-01

Knowing the parameters conditioning the critical speed of a pump shaft is important, both for safety and design purposes, since the shafts are often to operate beyond the first critical speed. These aims led CEA, associated with NOVATOME and FRAMATOME (with the cooperation of JEUMONT-SCHNEIDER) to carry out a test program on critical speeds of a full scale nuclear pump shaft. Fluid-structure interaction plays an important part in the setting of critical speed. Due to the coupling between the rotative fluid flow and the transverse vibrations of the shaft, inertial and stiffness forces are created, which are non conservative and proportional to the added mass of the fluid. The hydrostatic bearing effect and the influence of the water carried along by the pump wheel were also investigated, but proved unimportant in the case of the shaft studied. Experimental results are compared with calculations of critical speed. (orig.)

Coaxial twin-shaft magnetic fluid seals applied in vacuum wafer-handling robot

Science.gov (United States)

Cong, Ming; Wen, Haiying; Du, Yu; Dai, Penglei

2012-07-01

Compared with traditional mechanical seals, magnetic fluid seals have unique characters of high airtightness, minimal friction torque requirements, pollution-free and long life-span, widely used in vacuum robots. With the rapid development of Integrate Circuit (IC), there is a stringent requirement for sealing wafer-handling robots when working in a vacuum environment. The parameters of magnetic fluid seals structure is very important in the vacuum robot design. This paper gives a magnetic fluid seal device for the robot. Firstly, the seal differential pressure formulas of magnetic fluid seal are deduced according to the theory of ferrohydrodynamics, which indicate that the magnetic field gradient in the sealing gap determines the seal capacity of magnetic fluid seal. Secondly, the magnetic analysis model of twin-shaft magnetic fluid seals structure is established. By analyzing the magnetic field distribution of dual magnetic fluid seal, the optimal value ranges of important parameters, including parameters of the permanent magnetic ring, the magnetic pole tooth, the outer shaft, the outer shaft sleeve and the axial relative position of two permanent magnetic rings, which affect the seal differential pressure, are obtained. A wafer-handling robot equipped with coaxial twin-shaft magnetic fluid rotary seals and bellows seal is devised and an optimized twin-shaft magnetic fluid seals experimental platform is built. Test result shows that when the speed of the two rotational shafts ranges from 0-500 r/min, the maximum burst pressure is about 0.24 MPa. Magnetic fluid rotary seals can provide satisfactory performance in the application of wafer-handling robot. The proposed coaxial twin-shaft magnetic fluid rotary seal provides the instruction to design high-speed vacuum robot.

Design considerations for sealing the shafts of a nuclear fuel waste disposal vault

International Nuclear Information System (INIS)

Mortazavi, M.H.S.; Chan, H.T.; Radhakrishna, H.S.

1985-05-01

The shafts in an underground disposal system, which constitute potential pathways between the disposal vault and the biosphere, should be effectively sealed if the system is to perform as a hydrodynamic and geochemical barrier for the safe containment of nuclear fuel waste. In the design of the shaft backfill, consideration should be given to ensure that the backfill and the backfill/rock interface remain intact. Design-related problems, including critical pathways for the transport or radionuclides, configuration of shaft backfill and its functional requirements, the state of stress in a backfilled shaft with particular emphasis on the arching and load transfer phenomenon are discussed in this report

Shaft Seal Compensates for Cold Flow

Science.gov (United States)

Myers, W. N.; Hein, L. A.

1985-01-01

Seal components easy to install. Ring seal for rotating or reciprocating shafts spring-loaded to compensate for slow yielding (cold flow) of sealing material. New seal relatively easy to install because components preassembled, then installed in one piece.

Correlation of operating parameters on turbine shaft vibrations

Science.gov (United States)

Dixit, Harsh Kumar; Rajora, Rajeev

2016-05-01

The new generation of condition monitoring and diagnostics system plays an important role in efficient functioning of power plants. In most of the rotating machine, defects can be detected by such a system much before dangerous situation occurs. It allows the efficient use of stationary on-line continuous monitoring system for condition monitoring and diagnostics as well. Condition monitoring of turbine shaft can not only reduce expenses of maintenance of turbo generator of power plants but also prevents likely shutdown of plant, thereby increases plant load factor. Turbo visionary parameters are essential part of health diagnosis system of turbo generator. Particularly steam pressure, steam temperature and lube oil temperature are important parameters to monitor because they are having much influence on turbine shaft vibration and also governing systems are available for change values of those parameters. This paper includes influence of turbo visionary parameters i.e., steam temperature, steam pressure, lube oil temperature, turbine speed and load on turbine shaft vibration at turbo generator at 195 MW unit-6,Kota Super Thermal Power Station by measuring vibration amplitude and analyze them in MATLAB.

Analysis of the Dynamic Behavior of a Rotating Composite Hollow Shaft

Directory of Open Access Journals (Sweden)

Aldemir Ap Cavalini Jr

Full Text Available Abstract In the present paper, a simplified homogenized beam theory is used in the context of a numerical investigation regarding the dynamic behavior of a rotating composite hollow shaft. For this aim, a horizontal flexible composite shaft and a rigid disc form the considered simple supported rotating system. The mathematical model of the rotor is derived from the Lagrange’s equation and the Rayleigh-Ritz method, which is obtained from the strain and kinetic energies of the disc and shaft, and the mass unbalance. In this case, a convergence procedure is carried out in terms of the vibration modes to obtain a representative model for the rotor system. Therefore, the proposed analysis is performed in both frequency and time domains, in which the frequency response functions, the unbalance responses, the Campbell diagram, and the orbits are numerically determined. Additionally, the instability threshold of the rotor system is obtained. This study illustrates the convenience of the composite hollow shafts for rotor dynamics applications.

Accessibility Changes within Diphtheria Toxin T Domain upon Membrane Penetration Probed by Hydrogen Exchange and Mass Spectrometry

Czech Academy of Sciences Publication Activity Database

Man, Petr; Montagner, C.; Vitrac, H.; Kavan, Daniel; Pichard, S.; Gillet, D.; Forest, E.; Forge, V.

2011-01-01

Roč. 414, č. 1 (2011), s. 123-134 ISSN 0022-2836 Institutional research plan: CEZ:AV0Z50200510 Keywords : diphtheria toxin * translocation domain * protein/membrane interactions Subject RIV: CE - Biochemistry Impact factor: 4.001, year: 2011

Seroepidemiology of diphtheria, tetanus, poliomyelitis and pertussis : evaluation of the national immunisation programme in the Netherlands

NARCIS (Netherlands)

Melker, de H.

1999-01-01

In view of the evaluation of the National Immunisation Programme in the Netherlands the main objectives were to obtain insight into the immunity to diphtheria, tetanus and poliomyelitis, into the occurrence of pertussis and to improve serodiagnosis of pertussis.

In a

Direct shaft torque measurements in a transient turbine facility

International Nuclear Information System (INIS)

Beard, Paul F; Povey, Thomas

2011-01-01

This paper describes the development and implementation of a shaft torque measurement system for the Oxford Turbine Research Facility (formerly the Turbine Test Facility (TTF) at QinetiQ, Farnborough), or OTRF. As part of the recent EU TATEF II programme, the facility was upgraded to allow turbine efficiency measurements to be performed. A shaft torque measurement system was developed as part of this upgrade. The system is unique in that, to the authors' knowledge, it provided the first direct measurement of shaft torque in a transient turbine facility although the system has wider applicability to rotating test facilities in which power measurement is a requirement. The adopted approach removes the requirement to quantify bearing friction, which can be difficult to accurately calibrate under representative operating conditions. The OTRF is a short duration (approximately 0.4 s run time) isentropic light-piston facility capable of matching all of the non-dimensional parameters important for aerodynamic and heat studies, namely Mach number, Reynolds number, non-dimensional speed, stage pressure ratio and gas-to-wall temperature ratio. The single-stage MT1 turbine used for this study is a highly loaded unshrouded design, and as such is relevant to modern military, or future civil aero-engine design. Shaft torque was measured directly using a custom-built strain gauge-based torque measurement system in the rotating frame of reference. This paper describes the development of this measurement system. The system was calibrated, including the effects of temperature, to a traceable primary standard using a purpose-built facility. The bias and precision uncertainties of the measured torque were ±0.117% and ±0.183%, respectively. To accurately determine the shaft torque developed by a turbine in the OTRF, small corrections due to inertial torque (associated with changes in the rotational speed) and aerodynamic drag (windage) are required. The methods for performing these

Construction of full-scale shaft seals in crystalline rock

International Nuclear Information System (INIS)

Martino, J.B.; Dixon, D.A.; Onagi, D.; Kim, C-S; Holowick, B.

2011-01-01

The Underground Research Laboratory (URL) was constructed to investigate concepts related to geological disposal of used nuclear fuel. This involved investigating the rock mass by undertaking in situ experiments using a multi disciplinary technical approach. The decision was made in 2003 to close the URL. Part of the closure process involved installing seals in the access and ventilation shafts at locations where they intersected an ancient thrust fault (Fracture Zone 2 -- FZ2). FZ2 is an active hydraulic pathway in the Lac du Bonnet batholith and this feature is the dominating structural and hydrogeological feature at the URL site. Above FZ2 the groundwater has a low salinity and is dominated by surface-related processes. With increasing depth in the batholith, surface-related effects decrease and the salinity of the groundwater increases to 90 g/L total dissolved solids. The decision to install the seals was made as part of the due diligence for the site closure to ensure that the saline groundwater located at depth down strike and below the thrust fault would not enter the closed underground openings and mix with less saline shallow groundwater. The construction of each seal involved the installation of a heavily reinforced low alkalinity concrete component keyed into the surrounding rock. The concrete supported and restrained a central clay-sand component, which was capped by an unreinforced concrete component. The clay-sand component spans the exposure of the thrust fault in each shaft. This paper describes the construction of the main shaft seal and the ventilation shaft seal. The construction of the shaft seals at the URL was part of the Nuclear Legacy Liabilities Program (NLLP) being funded by Natural Resources Canada (NRCan). (author)

Construction of full-scale shaft seals in crystalline rock

Energy Technology Data Exchange (ETDEWEB)

Martino, J.B.; Dixon, D.A.; Onagi, D.; Kim, C-S; Holowick, B. [Atomic Energy of Canada Limited, Pinawa, MB (Canada)

2011-07-01

The Underground Research Laboratory (URL) was constructed to investigate concepts related to geological disposal of used nuclear fuel. This involved investigating the rock mass by undertaking in situ experiments using a multi disciplinary technical approach. The decision was made in 2003 to close the URL. Part of the closure process involved installing seals in the access and ventilation shafts at locations where they intersected an ancient thrust fault (Fracture Zone 2 -- FZ2). FZ2 is an active hydraulic pathway in the Lac du Bonnet batholith and this feature is the dominating structural and hydrogeological feature at the URL site. Above FZ2 the groundwater has a low salinity and is dominated by surface-related processes. With increasing depth in the batholith, surface-related effects decrease and the salinity of the groundwater increases to 90 g/L total dissolved solids. The decision to install the seals was made as part of the due diligence for the site closure to ensure that the saline groundwater located at depth down strike and below the thrust fault would not enter the closed underground openings and mix with less saline shallow groundwater. The construction of each seal involved the installation of a heavily reinforced low alkalinity concrete component keyed into the surrounding rock. The concrete supported and restrained a central clay-sand component, which was capped by an unreinforced concrete component. The clay-sand component spans the exposure of the thrust fault in each shaft. This paper describes the construction of the main shaft seal and the ventilation shaft seal. The construction of the shaft seals at the URL was part of the Nuclear Legacy Liabilities Program (NLLP) being funded by Natural Resources Canada (NRCan). (author)

Magnetostrictive patch sensor system for battery-less real-time measurement of torsional vibrations of rotating shafts

Science.gov (United States)

Lee, Jun Kyu; Seung, Hong Min; Park, Chung Il; Lee, Joo Kyung; Lim, Do Hyeong; Kim, Yoon Young

2018-02-01

Real-time uninterrupted measurement for torsional vibrations of rotating shafts is crucial for permanent health monitoring. So far, strain gauge systems with telemetry units have been used for real-time monitoring. However, they have a critical disadvantage in that shaft operations must be stopped intermittently to replace telemetry unit batteries. To find an alternative method to carry out battery-less real-time measurement for torsional vibrations of rotating shafts, a magnetostrictive patch sensor system was proposed in the present study. Since the proposed sensor does not use any powered telemetry system, no battery is needed and thus there is no need to stop rotating shafts for battery replacement. The proposed sensor consists of magnetostrictive patches and small magnets tightly bonded onto a shaft. A solenoid coil is placed around the shaft to convert magnetostrictive patch deformation by shaft torsional vibration into electric voltage output. For sensor design and characterization, investigations were performed in a laboratory on relatively small-sized stationary solid shaft. A magnetostrictive patch sensor system was then designed and installed on a large rotating propulsion shaft of an LPG carrier ship in operation. Vibration signals were measured using the proposed sensor system and compared to those measured with a telemetry unit-equipped strain gauge system.

Safety and immunogenicity of a combined Tetanus, Diphtheria, recombinant acellular Pertussis vaccine (TdaP) in healthy Thai adults.

Science.gov (United States)

Sirivichayakul, Chukiat; Chanthavanich, Pornthep; Limkittikul, Kriengsak; Siegrist, Claire-Anne; Wijagkanalan, Wassana; Chinwangso, Pailinrut; Petre, Jean; Hong Thai, Pham; Chauhan, Mukesh; Viviani, Simonetta

2017-01-02

An acellular Pertussis (aP) vaccine containing recombinant genetically detoxified Pertussis Toxin (PTgen), Filamentous Hemagglutinin (FHA) and Pertactin (PRN) has been developed by BioNet-Asia (BioNet). We present here the results of the first clinical study of this recombinant aP vaccine formulated alone or in combination with tetanus and diphtheria toxoids (TdaP). A phase I/II, observer-blind, randomized controlled trial was conducted at Mahidol University in Bangkok, Thailand in healthy adult volunteers aged 18-35 y. The eligible volunteers were randomized to receive one dose of either BioNet's aP or Tetanus toxoid-reduced Diphtheria toxoid-acellular Pertussis (TdaP) vaccine, or the Tdap Adacel® vaccine in a 1:1:1 ratio. Safety follow-up was performed for one month. Immunogenicity was assessed at baseline, at 7 and 28 d after vaccination. Anti-PT, anti-FHA, anti-PRN, anti-tetanus and anti-diphtheria IgG antibodies were assessed by ELISA. Anti-PT neutralizing antibodies were assessed also by CHO cell assay. A total of 60 subjects (20 per each vaccine group) were enrolled and included in the safety analysis. Safety laboratory parameters, incidence of local and systemic post-immunization reactions during 7 d after vaccination and incidence of adverse events during one month after vaccination were similar in the 3 vaccine groups. One month after vaccination, seroresponse rates of anti-PT, anti-FHA and anti-PRN IgG antibodies exceeded 78% in all vaccine groups. The anti-PT IgG, anti-FHA IgG, and anti-PT neutralizing antibody geometric mean titers (GMTs) were significantly higher following immunization with BioNet's aP and BioNet's TdaP than Adacel® (Pdiphtheria GMTs at one month after immunization were comparable in all vaccine groups. All subjects had seroprotective titers of anti-tetanus and anti-diphtheria antibodies at baseline. In this first clinical study, PTgen-based BioNet's aP and TdaP vaccines showed a similar tolerability and safety profile to Adacel

Comparison of tibial shaft ski fractures in children and adults.

Science.gov (United States)

Hamada, Tomo; Matsumoto, Kazu; Ishimaru, Daichi; Sumi, Hiroshi; Shimizu, Katsuji

2014-09-01

To examine whether child and adult skiers have different risk factors or mechanisms of injury for tibial shaft fractures. Descriptive epidemiological study. Prospectively analyzed the epidemiologic factors, injury types, and injury mechanisms at Sumi Memorial Hospital. This study analyzed information obtained from 276 patients with tibial fractures sustained during skiing between 2004 and 2012. We focused on 174 ski-related tibial shaft fractures with respect to the following factors: age, gender, laterality of fracture, skill level, mechanism of fracture (fall vs collision), scene of injury (steepness of slope), snow condition, and weather. Fracture pattern was graded according to Arbeitsgemeinschaft für Osteosynthesefragen (AO) classification and mechanical direction [external (ER) or internal rotation (IR)]. Tibial shaft fractures were the most common in both children (89.3%) and adults (47.4%). There were no significant differences in gender, side of fracture, mechanism of fracture, snow condition, or weather between children and adults. Skill levels were significantly lower in children than in adults (P differences in some of these parameters, suggesting that child and adult skiers have different risk factors or mechanisms of injury for tibial shaft fractures.

Exploratory shaft conceptual design report: Gulf Interior Region salt domes

International Nuclear Information System (INIS)

1983-07-01

This conceptual design report summarizes the conceptualized design for an exploratory shaft facility at a representative site in the Gulf Interior Region of the United States (Louisiana and Mississippi). Conceptualized designs for other possible locations (Paradox Basin in Utah and Permian Basin in Texas) are summarized in separate reports. The purpose of the exploratory shaft facility is to provide access to the reference repository horizon to permit in-situ testing of the salt. The in-situ testing is necessary to verify repository salt design parameters, evaluate isotropy and homoqeneity of the salt, and provide a demonstration of the constructability and confirmation of the design to gain access to the repository. The fundamental purpose of this conceptual design report is to assure the feasibility of the exploratory shaft project and to develop a reliable cost estimate and realistic schedule. Because a site has not been selected and site-specific subsurface data are not available, it has been necessary to make certain assumptions in order to develop a conceptural design for an exploratory shaft facility in salt. As more definitive information becomes available to support the design process, adjustments in the projected schedule and estimated costs will be required

Rotary compression process for producing toothed hollow shafts

Directory of Open Access Journals (Sweden)

J. Tomczak

2014-10-01

Full Text Available The paper presents the results of numerical analyses of the rotary compression process for hollow stepped shafts with herringbone teeth. The numerical simulations were performed by Finite Element Method (FEM, using commercial software package DEFORM-3D. The results of numerical modelling aimed at determining the effect of billet wall thickness on product shape and the rotary compression process are presented. The distributions of strains, temperatures, damage criterion and force parameters of the process determined in the simulations are given, too. The numerical results obtained confirm the possibility of producing hollow toothed shafts from tube billet by rotary compression methods.

Improved circumferential shaft seal for aircraft gear transmissions

Science.gov (United States)

Ludwig, L. P.; Strom, T. N.

1973-01-01

Operation under simulated aircraft transmission conditions of speeds to 2850 m/min (9350 ft/min), lubricant temperatures to 394 K (250 F), shaft radial runouts to 0.254 mm (0.010 in.) F.I.R. (full indicator reading), and pressure differentials to 1.03 N/cm2 (1.5 psi) revealed that conventional circumferential seals leaked excessively. Modifying the conventional seal by adding helical grooves to the seal bore reduced leakage rates to within the acceptable level of 10 cm3/hr. The leakage rate of this modified seal was not significantly affected by lubricant flooding or by shaft radial runout.

Shaft sealing issue in CO2 storage sites

Science.gov (United States)

Dieudonné, A.-C.; Charlier, R.; Collin, F.

2012-04-01

Carbon capture and storage is an innovating approach to tackle climate changes through the reduction of greenhouse gas emissions. Deep saline aquifers, depleted oil and gas reservoirs and unmineable coal seams are among the most studied reservoirs. However other types of reservoir, such as abandonned coal mines, could also be used for the storage of carbon dioxide. In this case, the problem of shaft sealing appears to be particularly critical regarding to the economic, ecologic and health aspects of geological storage. The purpose of the work is to study shaft sealing in the framework of CO2 storage projects in abandoned coal mines. The problem of gas transfers around a sealing system is studied numerically using the finite elements code LAGAMINE, which has been developped for 30 years at the University of Liege. A coupled hydro-mechanical model of unsaturated geomaterials is used for the analyses. The response of the two-phase flow model is first studied through a simple synthetic problem consisting in the injection of gas in a concrete-made column. It stands out of this first modeling that the advection of the gas phase represents the main transfer mechanism of CO2 in highly unsaturated materials. Furthermore the setting of a bentonite barrier seal limits considerably the gas influx into the biosphere. A 2D axisymetric hydromechanical modeling of the Anderlues natural gas storage site is then performed. The geological and hydrogeological contexts of the site are used to define the problem, for the initial and boundary conditions, as well as the material properties. In order to reproduce stress and water saturation states in the shale before CO2 injection in the mine, different phases corresponding to the shaft sinking, the mining and the set up of the sealing system are simulated. The system efficiency is then evaluated by simulating the CO2 injection with the imposed pressure at the shaft wall. According to the modeling, the low water saturation of concrete and

Optimal hydraulic design of new-type shaft tubular pumping system

International Nuclear Information System (INIS)

Zhu, H G; Zhang, R T; Zhou, J R

2012-01-01

Based on the characteristics of large flow rate, low-head, short annual operation time and high reliability of city flood-control pumping stations, a new-type shaft tubular pumping system featuring shaft suction box, siphon-type discharge passage with vacuum breaker as cutoff device was put forward, which possesses such advantages as simpler structure, reliable cutoff and higher energy performance. According to the design parameters of a city flood control pumping station, a numerical computation model was set up including shaft-type suction box, siphon-type discharge passage, pump impeller and guide vanes. By using commercial CFD software Fluent, RNG κ-ε turbulence model was adopted to close the three-dimensional time-averaged incompressible N-S equations. After completing optimal hydraulic design of shaft-type suction box, and keeping the parameters of total length, maximum width and outlet section unchanged, siphon-type discharge passages of three hump locations and three hump heights were designed and numerical analysis on the 9 hydraulic design schemes of pumping system were proceeded. The computational results show that the changing of hump locations and hump heights directly affects the internal flow patterns of discharge passages and hydraulic performances of the system, and when hump is located 3.66D from the inlet section and hump height is about 0.65D (D is the diameter of pump impeller), the new-type shaft tubular pumping system achieves better energy performances. A pumping system model test of the optimal designed scheme was carried out. The result shows that the highest pumping system efficiency reaches 75.96%, and when at design head of 1.15m the flow rate and system efficiency were 0.304m 3 /s and 63.10%, respectively. Thus, the validity of optimal design method was verified by the model test, and a solid foundation was laid for the application and extension of the new-type shaft tubular pumping system.

Interaction between drilled shaft and mechanically stabilized earth (MSE) wall : project summary.

Science.gov (United States)

2015-08-31

Drilled shafts are being constructed within the reinforced zone of mechanically stabilized earth (MSE) walls (Figure 1). The drilled shafts may be subjected to horizontal loads and push against the front of the wall. Distress of MSE wall panels has b...

The detection of wind turbine shaft misalignment using temperature monitoring

OpenAIRE

Tonks, Oliver; Wang, Qing

2016-01-01

Temperature is a parameter increasingly monitored in wind turbine systems. This paper details a potential temperature monitoring technique for use on shaft couplings. Such condition monitoring methods aid fault detection in other areas of wind turbines. However, application to shaft couplings has not previously been widely researched. A novel temperature measurement technique is outlined, using an infra-red thermometer which can be applied to online condition monitoring. The method was va...

Granuloma annulare localized to the shaft of the penis

DEFF Research Database (Denmark)

Trap, R; Wiebe, B

1993-01-01

A case of granuloma annulare localized to the shaft of the penis is reported. The differential diagnoses are discussed. Penile granuloma annulare is a rare disorder and it is concluded that biopsies of penile lesions are recommended to verify the correct diagnosis.......A case of granuloma annulare localized to the shaft of the penis is reported. The differential diagnoses are discussed. Penile granuloma annulare is a rare disorder and it is concluded that biopsies of penile lesions are recommended to verify the correct diagnosis....

Two cases of cutaneous diphtheria associated with occupational pig contact in Germany.

Science.gov (United States)

Berger, A; Boschert, V; Konrad, R; Schmidt-Wieland, T; Hörmansdorfer, S; Eddicks, M; Sing, A

2013-12-01

In 2010, two independent cases of cutaneous diphtheria caused by toxigenic C. ulcerans were identified in Germany. Both patients had intense occupational contact with pigs. Diagnostic work-up comprising biochemical differentiation, rpoB sequencing, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) analysis, real-time tox PCR and Elek test as well as public health measures including an intensified source tracing involving 83 asymptomatic pigs of an associated pig farm are presented. © 2012 Blackwell Verlag GmbH.

Effect of diphtheria toxin T-domain on endosomal pH

Directory of Open Access Journals (Sweden)

A. J. Labyntsev

2015-08-01

Full Text Available A key step in the mode of cytotoxic action of diphtheria toxin (DT is the transfer of its catalytic domain (Cd from endosomes into the cytosol. The main activity in this process is performed by the transport domain (Td, but the molecular mechanism of its action remains unknown. We have previously shown that Td can have some influence on the endosomal transport of DT. The aim of this work was to study the effect of diphtheria toxin on the toxin compartmentalization in the intracellular transporting pathway and endosomal pH. We used recombinant fragments of DT, which differed only by the presence of Td in their structure, fused with fluorescent proteins. It was shown that the toxin fragment with Td moved slower by the pathway early-late endosomes-lysosomes, and had a slightly different pattern of colocalization with endosomal markers than DT fragment without Td. In addition, endosomes containing DT fragments with Td had a constant pH of about 6.5 from the 10th to 50th minute of observation, for the same time endosomes containing DT fragments without Td demons­trated a decrease in pH from 6.3 to 5.5. These results indicate that Td inhibits acidification of endosomal medium. One of possible explanations for this may be the effect of the ion channel formed by the T-domain on the process of the endosomal acidification. This property of Td may not only inhibit maturation of endosomes but also inhibit activation of endosomal pH-dependent proteases, and this promotes successful transport of Cd into the cell cytosol.

Shaft seals for final high-level radioactive waste repositories. ELSA. Pt. 1

International Nuclear Information System (INIS)

Kudla, W.; Schreiter, F.; Gruner, M.

2013-01-01

The state of the art in science and technology fir shaft seals with long-term stability is summarized regarding their applicability for high-level waste repository in Germany. The concepts and drafts for the shaft sealing systems ERAM, Asse, Konrad, the WIPP side, the RESEAL concept, the NAGRA concept and the project LASA are reviewed. The methodology of applying partial factors in a safety analysis is summarized and the applicability of this method for geotechnical sealing structures is confirmed. To establish geomechanical boundary conditions of the host rocks and clay stone the stress-strain behavior of the rock mass adjoining the shaft has to be identified including time-dependent thermo-mechanical processes. The general and special requirements for the design of shaft sealing systems, especially in salt rock and clay formations are described, derived from the safety requirements (BMU 2010). Finally general information needs were identified.

Technique to reduce the shaft torque stress at an induction machine

Directory of Open Access Journals (Sweden)

Adrian Tulbure

2005-10-01

Full Text Available For the active attenuation at load stress in the drive shaft, the control system should receive as input signal the instantaneous shaft torque value. In this context an intelligent observer for shaft tongue of mains operatea induction machine, which is able to responding by variation of LIF (Load Input Function[1] must be developed. Extensive computer simulation prove the effectiveness of the proposed solution. In order to obtain a practical validation, the stimulated regulator has been designed and tested in the Institute of Electrical Engineering in Clausthal/Germany [2]. This paper contains following parts: Developing the mathematical model, Practical realisation, Simulations and measurements, Evaluating the control solutions and Conclusions.

Application of the cylindrically guided wave technique for bolt and pump-shaft inspections

International Nuclear Information System (INIS)

Light, G.M.; Ruescher, E.H.; Bloom, E.A.; Tsai, Y.M.

1990-01-01

Southwest Research Institute (SwRI) has been working with the cylindrically guided wave technique (CGWT) since late 1982. The initial work was aimed at inspecting reactor pressure vessel hold-down studs. The CGWT was shown to be able to detect defects as small as 0.060 inch (1.5 mm) deep through metal paths up to 120 inches (304 cm) in stud bolt carbon steel. Later developments in the application of CGWT were aimed at inspecting reactor coolant pump (RCP) shafts. The RCP shafts are usually approximately 2 meters long and have changing diameters along the length, from approximately 12 cm to 23 cm in discrete steps. The pump shafts have been susceptible to small cracks and can be inspected most cost-effectively from the top of the shaft. A matrix transducer composed of six 1-inch (2.54-cm) diameter transducers along with pulsing and receiving electronics (EPRI Pump-Shaft Inspection System) was developed during 1988. A patent application for this technology has been made. This report describes the work conducted during 1989 and the results obtained

Designing optimum diameter of skip shafts in mines with inclined or steep coal seams

Energy Technology Data Exchange (ETDEWEB)

Durov, E.M.

1981-07-01

This paper discusses methods of increasing depth of operating shaft mines considering optimization of hoisting systems. The following solutions are analyzed: removing mined rock material to the surface, to operating horizon, to the deepest horizon, removing rock to the deepest horizon by enlarging a large diameter borehole. It is suggested that removing rock material to the surface is most economical. This solution is sometimes difficult to implement due to design of mine shafts. If a shaft is equipped with two pairs of skips, or with a pair of skips and two independent skips, one skip or a pair of skips can be removed to form free space for buckets used to hoist mined rock and coal. The bucket moves along rope shaft guides. Analysis of the optimum hoisting systems in shaft mines for coal mines with the following design capacity is carried out: 0.9, 1.2, 1.5 and 1.8 Mmt a year. The following depth of working horizons is evaluated: 600, 800, 1000, 1200, 1400 and 1600 m. It is assumed that coal and rock are hoisted separately. Advance rate ranges from 10 to 50 m/month. The results of analysis are shown in two tables. It is suggested that from the point of view of increasing depth of active mine shafts the following solutions are optimum: 7 m shaft with a system of three independently moving skips (two for coal, one for rock material), and 8 m shaft equipped with a pair of skips and two independent skips (one of the independently moving skips is used for rock hoisting). 4 refs.

Rustler Formation in the waste handling and exhaust shafts, Waste Isolation Pilot Plant (WIPP) site, southeastern New Mexico

International Nuclear Information System (INIS)

Holt, R.M.; Powers, D.W.

1987-01-01

The Permian Rustler Formation was recently examined in detail in two shafts at the WIPP site: the waste handling shaft (waste shaft) and the exhaust shaft. Fresh exposures of the Rustler in the shafts exhibited abundant primary sedimentary structures. The abundance of primary sedimentary structures observed in the shafts is unequaled in previously described sections. Data are reported here in their stratigraphic context as an initial basis for evaluation of depositional environments of the Rustler and reevaluating the role of dissolution in the formation of the Rustler. 10 refs

Seismic fragility capacity of equipment--horizontal shaft pump test

International Nuclear Information System (INIS)

Iijima, T.; Abe, H.; Suzuki, K.

2005-01-01

The current seismic fragility capacity of horizontal shaft pump is 1.6 x 9.8 m/s 2 (1.6 g), which was decided from previous vibration tests and we believe that it must have sufficient margin. The purpose of fragility capacity test is to obtain realistic seismic fragility capacity of horizontal shaft pump by vibration tests. Reactor Building Closed Cooling Water (RCW) Pump was tested as a typical horizontal shaft pump, and then bearings and liner rings were tested as important parts to evaluate critical acceleration and dispersion. Regarding RCW pump test, no damage was found, though maximum input acceleration level was 6 x 9.8 m/s 2 (6 g). Some kinds of bearings and liner rings were tested on the element test. Input load was based on seismic motion which was same with the RCW pump test, and maximum load was equivalent to over 20 times of design seismic acceleration. There was not significant damage that caused emergency stop of pump but degradation of surface roughness was found on some kinds of bearings. It would cause reduction of pump life, but such damage on bearings occurred under large seismic load condition that was equivalent to over 10 to 20 g force. Test results show that realistic fragility capacity of horizontal shaft pump would be at least four times as higher as current value which has been used for our seismic PSA. (authors)

Space Shuttle Orbital Maneuvering Subsystem (OMS) Engine Propellant Leakage Ball-Valve Shaft Seals

Science.gov (United States)

Lueders, Kathy; Buntain, Nick; Fries, Joseph (Technical Monitor)

1999-01-01

Evidence of propellant leakage across ball-valve shaft seals has been noted during the disassembly of five flight engines and one test engine at the NASA Lyndon B. Johnson Space Center, White Sands Test Facility. Based on data collected during the disassembly of these five engines, the consequences of propellant leakage across the ball-valve shaft seals can be divided into four primary areas of concern: Damage to the ball-valve pinion shafts, damage to sleeved bearings inside the ball-valve and actuator assemblies, degradation of the synthetic rubber o-rings used in the actuator assemblies, and corrosion and degradation to the interior of the actuator assemblies. The exact time at which leakage across the ball-valve shaft seals occurs has not been determined, however, the leakage most likely occurs during engine firings when, depending on the specification used, ball-valve cavity pressures range as high as 453 to 550 psia. This potential pressure range for the ball-valve cavities greatly exceeds the acceptance leakage test pressure of 332 psia. Since redesign and replacement of the ball-valve shaft seals is unlikely, the near term solution to prevent damage that occurs from shaft-seal leakage is to implement a routine overhaul and maintenance program for engines in the fleet. Recommended repair, verification, and possible preventative maintenance measures are discussed in the paper.

Short-term and long-term antibody response by mice after immunization against Neisseria meningitidis B or diphtheria toxoid

Directory of Open Access Journals (Sweden)

G.P. Silva

2013-02-01

Full Text Available Serogroup B Neisseria meningitidis (MenB is a major cause of invasive disease in early childhood worldwide. The only MenB vaccine available in Brazil was produced in Cuba and has shown unsatisfactory efficacy when used to immunize millions of children in Brazil. In the present study, we compared the specific functional antibody responses evoked by the Cuban MenB vaccine with a standard vaccine against diphtheria (DTP: diphtheria, tetanus, pertussis after primary immunization and boosting of mice. The peak of bactericidal and opsonic antibody titers to MenB and of neutralizing antibodies to diphtheria toxoid (DT was reached after triple immunization with the MenB vaccine or DTP vaccine, respectively. However, 4 months after immunization, protective DT antibody levels were present in all DTP-vaccinated mice but in only 20% of the mice immunized against MenB. After 6 months of primary immunization, about 70% of animals still had protective neutralizing DT antibodies, but none had significant bactericidal antibodies to MenB. The booster doses of DTP or MenB vaccines produced a significant antibody recall response, suggesting that both vaccines were able to generate and maintain memory B cells during the period studied (6 months post-triple immunization. Therefore, due to the short duration of serological memory induced by the MenB vaccine (VA-MENGOC-BC® vaccine, its use should be restricted to outbreaks of meningococcal disease.

Implementation of a flaw model to the fracturing around a vertical shaft

CSIR Research Space (South Africa)

Van de Steen, B

2003-04-01

Full Text Available -scale excavations. The simulated fracture pattern around a vertical shaft is compared to the fracturing around a shaft at a depth of 3400 m. The simulations suggest that wedge-shaped zones, called dog-ears, a reformed by a progressive splitting-like failure...

Control rod drive shaft latch

International Nuclear Information System (INIS)

Thorp, A.G. II.

1976-01-01

A latch mechanism is operated by differential pressure on a piston to engage the drive shaft for a control rod in a nuclear reactor, thereby preventing the control rod from being ejected from the reactor in case of failure of the control rod drive mechanism housing which is subjected to the internal pressure in the reactor vessel. 6 claims, 4 drawing figures

Shaft seal assembly for high speed and high pressure applications

Science.gov (United States)

Hadt, W. F.; Ludwig, L. P. (Inventor)

1979-01-01

A seal assembly is provided for reducing the escape of fluids from between a housing and a shaft rotably mounted in the housing. The seal assembly comprises a pair of seal rings resiliently connected to each other and disposed in side-by-side relationship. In each seal ring, both the internal bore surface and the radial face which faces away from the other seal ring are provided with a plurality of equi-spaced recesses. The seal faces referred to are located adjacent a seating surface of the housing. Under normal operating conditions, the seal assembly is stationary with respect to the housing, and the recesses generate life, keep the assembly spaced from the rotating shaft and allow slip therebetween. The seal assembly can seize on the shaft, and slip will then occur between the radial faces and the housing.

Expected long-term outcome after a tibial shaft fracture

DEFF Research Database (Denmark)

Faergemann, C; Frandsen, P A; Röck, N D

1999-01-01

OBJECTIVE: A prospective study of 207 laymen and professionals answered a questionnaire regarding the expectations of the long-term outcome 6 months after a unilateral tibial shaft fracture. The aim was (1) to disclose the expected outcome after unilateral tibial shaft fracture, and (2) to compare...... these expectations with the outcome measured in patients. METHODS: There were five groups of nonpatients: (1) 42 orthopedic surgeons, (2) 36 physiotherapists, (3) 42 students, (4) 49 white collar workers, and (5) 38 blue collar workers. Outcome was measured by Sickness Impact Profile (SIP). The SIP scores were...

Hydromechanical transmission with three simple planetary assemblies, one sun gear being mounted on the output shaft and the other two on a common shaft connected to an input-driven hydraulic module

Science.gov (United States)

Orshansky, Jr., deceased, Elias; Weseloh, William E.

1978-01-01

A power transmission having three simple planetary assemblies, each having its own carrier and its own planet, sun, and ring gears. A speed-varying module is connected in driving relation to the input shaft and in driving relationship to the sun gears of the first two planetary assemblies, these two sun gears being connected together on a common shaft. The speed-varying means may comprise a pair of hydraulic units hydraulically interconnected so that one serves as a pump while the other serves as a motor and vice versa, one of the units having a variable stroke and being connected in driving relation to the input shaft, the other unit, which may have a fixed stroke, being connected in driving relation to the sun gears. The input shaft is also connected to drive the second ring gear and, furthermore is clutchable to the carrier of the third planetary assembly. A brake grounds the first carrier in the first range and in reverse and causes drive to be delivered to the output through the first ring gear in a hydrostatic mode. The carrier of the second planetary assembly drives the ring gear of the third planetary assembly, which is clutchable to the output shaft, and the sun gear of the third planetary assembly is mounted rigidly to the output shaft.

Less invasive plate osteosynthesis in humeral shaft fractures.

Science.gov (United States)

Apivatthakakul, Theerachai; Phornphutkul, Chanakarn; Laohapoonrungsee, Anupong; Sirirungruangsarn, Yuddhasert

2009-12-01

Stable internal fixation of the humeral shaft by less invasive percutaneous plate insertion using two separate (proximal and distal) incisions, indirect reduction by closed manipulation and fixation to preserve the soft tissue and blood supply at the fracture zone. Early mobilization of the shoulder and elbow to ensure a good functional outcome. Humeral shaft fractures (classified according to AO classification as: 12-A, B, C). Humeral shaft fractures extending to the proximal or distal shaft, small or deformed medullary canal or open growth plate. Humeral shaft fractures with primary radial nerve palsy. Proximal humeral shaft fractures extending to the humeral head. Distal humeral fractures extending to the elbow joint. Two incisions proximal and distal to the fracture zone are used. A 3-cm proximal incision lies between the lateral border of the proximal part of the biceps and the medial border of the deltoid. Distally, a 3-cm incision is made along the lateral border of the biceps. The interval between biceps and brachialis is identified. The biceps is retracted medially to expose the musculocutaneous nerve. The brachialis muscle has dual innervation, the medial half being innervated by the musculocutaneous nerve and the lateral half by the radial nerve. The brachialis is split longitudinally at its midline. The musculocutaneous nerve is retracted along with the medial half of the brachialis, while the lateral half of the brachialis serves as a cushion to protect the radial nerve. A deep subbrachial tunnel is created from the distal to the proximal incision. The selected plate is tied with a suture to a hole at the tip of the tunneling instrument for pulling the plate back along the prepared track. The plate is aligned in the correct position on the anterior surface of the humerus. Traction is applied and the fracture reduced to restore alignment by image intensifier, followed by plate fixation with at least two bicortical locking screws or three bicortical

Survey and analytical studies on a 'TAKANUKE' collapse mechanism for greatly deeper shafts (Contract research)

International Nuclear Information System (INIS)

Kurosaki, Yukio; Yamachi, Hiroshi; Matsui, Hiroya

2008-09-01

Mizunami underground research laboratory (MIU) is planned to be excavated to the depth of 1000m below the ground surface and is now under construction. One of the most serious problems in a greatly deeper shaft is 'TAKANUKE' collapse caused by slip movement of large discontinuities, as we have reported in the report of 'Study on Collapse Mechanism of Junction between Greatly Deeper Shaft and Horizontal Drifts [JAEA-Research 2008-248 (2008)]'. TAKANUKE collapse has been well known among mining engineers in JAPAN. However, an occurring mechanism of the collapse has not yet been revealed and a design code for it also has not been established. In this report, we have conducted numerical studies using finite difference method in order to throw an objective light on a mechanism of TAKANUKE collapse. These studies show two different stress states in upper and lower side of a large discontinuities. In lower side, a minimum principal stress at shaft wall region drastically reduces due to shaft sinking. This might make shaft wall stability difficult in poor geological condition. Such a TAKANUKE collapse can be found in ventilation shaft projects of the ENASAN tunnel. In the another side of discontinuity, a slip movement along discontinuities takes place due to shaft sinking. This slip movement induces a typical TAKANUKE collapse, as we have reported in 2007. In order to evaluate a possibility of TAKANUKE collapse during MIU main shaft sinking, we have conducted a particle body analysis, which can estimate a brittle failure of hard rock, such as MIU construction site. A fault with a steeply dipping over 79 degree to the main shaft, discovered in a survey boring at MIU site, has a low potential of TAKANUKE collapse during shaft sinking. Beside, a fault with dip of 60 degree may easily slip in a form of TAKANUKE collapse. One CD-ROM is attached as an appendix. (J.P.N.)

Documentation and verification of the SHAFT code; Yucca Mountain Site Characterization Project

Energy Technology Data Exchange (ETDEWEB)

St.John, C.M. [Agapito (J.F.T.) and Associates, Inc., Grand Junction, CO (United States)

1991-12-01

The SHAFT code incorporates equations to compute stresses in a shaft liner when the rock through which a shaft passes is subject to known three-dimensional states of stress or strain. The deformation modes considered are hoop deformation, axial deformation, and shear on a plane normal to the shaft axis. Interaction between the liner and the soil and rock is considered, and it is assumed that the liner is in place before loading is applied. This code is intended to be used interactively but creates a permanent record complete with necessary quality assurance information. The code has been carefully verified for the case of generalized plane strain, in which an arbitrary axial strain can be defined. It may also be used for plane stress analysis. Output is given in the form of stresses at selected sample points in the linear and the rock and a simple graphical representation of the distribution of stress through the liner. 12 figs., 13 tabs.

Mechanism of magnetic liquid flowing in the magnetic liquid seal gap of reciprocating shaft

Science.gov (United States)

Li, Decai; Xu, Haiping; He, Xinzhi; Lan, Huiqing

2005-03-01

In order to solve the problems that exist in the magnetic liquid seal of reciprocating shaft, we have set up an experimental facility, which composes a camera, microscope, step-by-step motor, pin roller screw, reciprocating motion shaft, pole pieces, permanent magnet and the magnetic liquid in the seal gap. Through the optical technology and image process of the experimental facility, we have studied the magnetic liquid flow in the seal gap when the reciprocating shaft moves with different velocities and strokes. This study specially concentrates on: (1) the regular pattern of such flow; (2) the loss quantity of magnetic liquid caused by the reciprocating motion shaft; (3) the failure reasons of this magnetic liquid seal; and (4) the design of a new structure for the magnetic liquid seal of reciprocating shaft. The application indicates that the new structure is very effective in some occasions. The new structure was accepted as the state patent in 2001 and authenticated as the achievement in the scientific research in 2002.

Mechanism of magnetic liquid flowing in the magnetic liquid seal gap of reciprocating shaft

International Nuclear Information System (INIS)

Li Decai; Xu Haiping; He Xinzhi; Lan Huiqing

2005-01-01

In order to solve the problems that exist in the magnetic liquid seal of reciprocating shaft, we have set up an experimental facility, which composes a camera, microscope, step-by-step motor, pin roller screw, reciprocating motion shaft, pole pieces, permanent magnet and the magnetic liquid in the seal gap. Through the optical technology and image process of the experimental facility, we have studied the magnetic liquid flow in the seal gap when the reciprocating shaft moves with different velocities and strokes. This study specially concentrates on: (1) the regular pattern of such flow; (2) the loss quantity of magnetic liquid caused by the reciprocating motion shaft; (3) the failure reasons of this magnetic liquid seal; and (4) the design of a new structure for the magnetic liquid seal of reciprocating shaft. The application indicates that the new structure is very effective in some occasions. The new structure was accepted as the state patent in 2001 and authenticated as the achievement in the scientific research in 2002

Hydro-mechanical modelling of a shaft seal in crystalline and sedimentary host rock media using COMSOL

Energy Technology Data Exchange (ETDEWEB)

Priyanto, D.G. [Atomic Energy of Canada Limited, Pinawa, MB (Canada)

2011-07-01

Shaft seals are components of the engineered barriers system considered for closure of a Deep Geological Repository (DGR). These seals would be installed in strategic locations of the shafts, where significant fracture zones (FZ) are located and would serve to limit upward flow of groundwater from the repository level towards the surface. This paper presents the results of hydro-mechanical (HM) numerical modelling exercises to evaluate the performance of a shaft seal using a finite element computer code, COMSOL. This study considered a variety of host geological media as part of generic assessments of system evolution in a variety of environments including five hypothetical sedimentary and crystalline host rock conditions. Four simulations of a shaft seal in different sedimentary rocks were completed, including: shale with isotropic permeability; shale with anisotropic permeability; limestone with isotropic permeability; and limestone with anisotropic permeability. The other simulation was a shaft seal in crystalline rock with isotropic permeability. Two different stages were considered in these HM simulations. Stages 1 and 2 simulated the groundwater flow into an open shaft and after installation of shaft sealing components, respectively. As expected, the models were able to simulate that installation of the shaft seal limits groundwater flow through the shaft. Based on the conditions and assumptions defined for the host media and fracture features examined in this study, the following conclusions can be drawn from the results of the numerical modelling exercises. A shaft that remained open for a longer time was beneficial with respect to delaying of seal saturation because it could reduce the groundwater flow rate around the fracture zone. Delaying saturation time indicates slower movement of the groundwater or other substances that may be transported with the groundwater. The core of the shaft seal (i.e., the bentonite-sand mixture (BSM)) became fully saturated

Load and resistance factor design of drilled shafts in shale for lateral loading.

Science.gov (United States)

2014-04-01

A research project involving 32 drilled shaft load tests was undertaken to establish LRFD procedures for : design of drilled shafts subjected to lateral loads. Tests were performed at two Missouri Department of : Transportation (MoDOT) geotechnical r...

A shaft seal system for the Waste Isolation Pilot Plant

International Nuclear Information System (INIS)

Hansen, F.D.; Ahrens, E.H.; Dennis, A.W.; Hurtado, L.D.; Knowles, M.K.; Tillerson, J.R.; Thompson, T.W.; Galbraith, D.

1996-01-01

As part of the demonstration of compliance with federal regulations, a shaft seal system has been designed for the Waste Isolation Pilot Plant. The system completely fills the 650 m shafts with components consisting of the common engineering materials, each of which possesses low permeability, longevity, and can be constructed using available technology. Design investigations couple rock mechanics and fluid flow analysis and tests of these materials within the natural geological setting, and demonstrate the effectiveness of the design

Designing vertical shafts for mines with deepening and outlet of rock to the operating level

Energy Technology Data Exchange (ETDEWEB)

Durov, Ye.M.

1983-01-01

Requirements for designing the cross sections of central shafts are examined, as well as those for determining the productivity of lifts and equipment of the shafts with a condition of use in a subsequent technological layout of their deepening with outlet of the rock to the operating level. Typical cross sections of skip and cellular shafts are given.

Bentonite as a waste isolation pilot plant shaft sealing material

International Nuclear Information System (INIS)

Daemen, J.; Ran, Chongwei

1996-12-01

Current designs of the shaft sealing system for the Waste Isolation Pilot Plant (WIPP) propose using bentonite as a primary sealing component. The shaft sealing designs anticipate that compacted bentonite sealing components can perform through the 10,000-year regulatory period and beyond. To evaluate the acceptability of bentonite as a sealing material for the WIPP, this report identifies references that deal with the properties and characteristics of bentonite that may affect its behavior in the WIPP environment. This report reviews published studies that discuss using bentonite as sealing material for nuclear waste disposal, environmental restoration, toxic and chemical waste disposal, landfill liners, and applications in the petroleum industry. This report identifies the physical and chemical properties, stability and seal construction technologies of bentonite seals in shafts, especially in a saline brine environment. This report focuses on permeability, swelling pressure, strength, stiffness, longevity, and densification properties of bentonites

Bentonite as a waste isolation pilot plant shaft sealing material

Energy Technology Data Exchange (ETDEWEB)

Daemen, J.; Ran, Chongwei [Univ. of Nevada, Reno, NV (United States)

1996-12-01

Current designs of the shaft sealing system for the Waste Isolation Pilot Plant (WIPP) propose using bentonite as a primary sealing component. The shaft sealing designs anticipate that compacted bentonite sealing components can perform through the 10,000-year regulatory period and beyond. To evaluate the acceptability of bentonite as a sealing material for the WIPP, this report identifies references that deal with the properties and characteristics of bentonite that may affect its behavior in the WIPP environment. This report reviews published studies that discuss using bentonite as sealing material for nuclear waste disposal, environmental restoration, toxic and chemical waste disposal, landfill liners, and applications in the petroleum industry. This report identifies the physical and chemical properties, stability and seal construction technologies of bentonite seals in shafts, especially in a saline brine environment. This report focuses on permeability, swelling pressure, strength, stiffness, longevity, and densification properties of bentonites.

Geologic mapping of the air intake shaft at the Waste Isolation Pilot Plant

International Nuclear Information System (INIS)

Holt, R.M.; Powers, D.W.

1990-12-01

The air intake shaft (AS) was geologically mapped from the surface to the Waste Isolation Pilot Plant (WIPP) facility horizon. The entire shaft section including the Mescalero Caliche, Gatuna Formation, Santa Rosa Formation, Dewey Lake Redbeds, Rustler Formation, and Salado Formation was geologically described. The air intake shaft (AS) at the Waste Isolation Pilot Plant (WIPP) site was constructed to provide a pathway for fresh air into the underground repository and maintain the desired pressure balances for proper underground ventilation. It was up-reamed to minimize construction-related damage to the wall rock. The upper portion of the shaft was lined with slip-formed concrete, while the lower part of the shaft, from approximately 903 ft below top of concrete at the surface, was unlined. As part of WIPP site characterization activities, the AS was geologically mapped. The shaft construction method, up-reaming, created a nearly ideal surface for geologic description. Small-scale textures usually best seen on slabbed core were easily distinguished on the shaft wall, while larger scale textures not generally revealed in core were well displayed. During the mapping, newly recognized textures were interpreted in order to refine depositional and post-depositional models of the units mapped. The objectives of the geologic mapping were to: (1) provide confirmation and documentation of strata overlying the WIPP facility horizon; (2) provide detailed information of the geologic conditions in strata critical to repository sealing and operations; (3) provide technical basis for field adjustments and modification of key and aquifer seal design, based upon the observed geology; (4) provide geological data for the selection of instrument borehole locations; (5) and characterize the geology at geomechanical instrument locations to assist in data interpretation. 40 refs., 27 figs., 1 tab

Torsional vibration analysis in turbo-generator shaft due to mal-synchronization fault

Science.gov (United States)

Bangunde, Abhishek; Kumar, Tarun; Kumar, Rajeev; Jain, S. C.

2018-03-01

A rotor of turbo-generator shafting is many times subjected to torsional vibrations during its lifespan. The reasons behind these vibrations are three-Phase fault, two-phase fault, line to ground fault, faulty-mal synchronization etc. Sometimes these vibrations can cause complete failure of turbo-generator shafting system. To calculate moment variation during these faults on the shafting system vibration analysis is done using Finite Elements Methods to calculate mass and stiffness matrix. The electrical disturbance caused during Mal-synchronization is put on generator section, and corresponding second order equations are solved by using “Duhamel Integral”. From the moment variation plots at four sections critically loaded sections are identified.

Transient Torsional Analysis of a Belt Conveyor Drive with Pneumatic Flexible Shaft Coupling

Directory of Open Access Journals (Sweden)

Kaššay Peter

2017-03-01

Full Text Available Development and application of pneumatic flexible shaft couplings have been in the center of our department research activities for a long time. These couplings are able to change torsional stiffness by changing pressure in their flexible elements – air bellows. Until now we have dealt with the use of pneumatic flexible shaft couplings for tuning mechanical systems working with periodically alternating load torque at steady state. Some mechanical systems, however, operate with a static load torque at constant speed (e.g. hoists, elevators, etc., where it is necessary to consider the suitability of shaft coupling in terms of load torque at transient conditions (run-up and braking. Therefore we decided to analyze the use of pneumatic flexible shaft couplings also in this type of mechanical systems on an example of conveyor belt drive.

Results from the geological surveys carried out in the Bure laboratory's shafts

International Nuclear Information System (INIS)

Rebours, Herve; Righini, Celine

2010-01-01

Document available in extended abstract form only. After the government's authorization to build and operate an underground laboratory, Andra started the investigation works in November 99 on the Meuse/Haute-Marne URL site. The Meuse/Haute-Marne URL is located at the border of the Champagne-Ardenne and Lorraine regions, on the township of Bure in the Callovo-Oxfordian clay-rich rock. On the URL site, the layer is about 135 m-thick and lies at a depth of 417 m to 552 m. The laboratory consists of two levels of experimental drifts at depths of 445 m and 490 m, respectively, with two vertical shafts crossing the 505 m-thick sedimentary cover of Kimeridgian (about 100 meters of marls and limestones), Oxfordian (about 300 meters of limestones) and Callovo-Oxfordian formations. The construction of the underground installations started in August 2000 with the sinking of the main shaft and was completed on the 27 April 2006 when it linked up with the southern drift of the laboratory. The two access shafts are sunk with a drill and blast method with steps of 2.4 to 3.1 m. A temporary support with grouted bolts and wire mesh is set immediately after the blasting and removal of the muck. The definitive concrete lining is installed about 12 to 20 m behind the face. The excavated diameter of the main shaft where the geological surveys and experiments have been undertaken is of 6 m (5 m after lining). The second shaft (auxiliary shaft for the ventilation of the URL) is sunk in a smaller diameter (5 m). The aims of the geological surveys carried out during the shaft sinking are to describe the vertical and lateral (between the two shafts) variations of the lithology, to confirm the absence of fault and the geometry of the argillaceous rocks formation. These surveys allow to characterize the natural or inducted fracturing by a sedimentary and structural follow-up of the excavation face. This follow-up was carried out every 2.4 to 3.0 meters in the shafts. During the shaft

A study on rock mass behaviour induced by shaft sinking in the Horonobe Underground Research Laboratory

International Nuclear Information System (INIS)

Tsusaka, Kimikazu; Tokiwa, Tetsuya; Inagaki, Daisuke; Hatsuyama, Yoshihiro; Koike, Masashi; Ijiri, Yuji

2012-01-01

Japan Atomic Energy Agency has been excavating three deep shafts through soft sedimentary rock in the Horonobe Underground Research Laboratory. In this paper, the authors discussed rock mass behaviour induced by a 6.5 m diameter shaft sinking. They conducted geological mapping in an excavation face and boreholes digged around the shaft wall, field measurements such as convergence measurements and monitoring of rock displacements using multi-interval borehole extensometers around a shaft at around 160 m and 220 m in depths, and three-dimensional numerical analysis which models the shaft excavation procedure such as timing of installation of support elements and setting and removal of a concrete form. As a result, it was clarified that remarkably large compressive strains occurred within about 1 m into the shaft wall in a radial direction since the rock mass behaviour was controlled by the concrete lining and that the behaviour would predominantly be induced by the fractures closing which opened significantly and propagated during excavation steps before the installation of a concrete lining and the directions where the strains occurred heavily depended on the fracture orientation around the shaft. (author)

Outcomes in closed reamed interlocking nail in fractures of shaft of femur

International Nuclear Information System (INIS)

Mohammad, T.; Sawati, A.; Ahmed, A.

2015-01-01

Femoral shaft fracture is one of the common fractures seen in accident and emergency department of our hospital. Violent forces are required to break this and strongest of human bones. There are various treatment modalities for femoral shaft fractures in adults like traction, brace, platting, intramedullary nail (IMN), external fixators and inter locking nails. The study was done with an objective to evaluate the results of closed reamed interlocking nail in fractures of shaft of femur. Methods: A prospective study of 114 cases of femoral shaft fractures was carried out at orthopaedic unit of Ayub Teaching Hospital Abbottabad during 1 year. All these cases were treated with statically locked nails under spinal or general anaesthesia. These cases were followed up for up to one year and Results of the interlocking nail were observed in terms of union and complications. Results: Out of 114 patients, 95 underwent union in 90-150 days with a mean of 110.68 days. Ten patients had dynamization within six weeks because of obvious fracture gap in radiograph. There were 3 patients who had non-union, and 6 patients had delayed union which was treated with dynamization. Conclusion: Close reamed interlocking intramedullary nail in femoral shaft fractures is the treatment of choice. Patient rehabilitation is early, hospitalization is short, and fracture healing response is good. (author)

Functional design criteria for an exploratory shaft facility in salt: Technical report

International Nuclear Information System (INIS)

1986-11-01

The purpose of the Functional Criteria for Design is to provide technical direction for the development of detailed design criteria for the exploratory shaft facility. This will assure that the exploratory shaft facility will be designed in accordance with the current Mission Plan as well as the Nuclear Waste Policy Act and 10 CFR Part 60, which will facilitate the licensing process. The functional criteria for design are not intended to limit or constrain the designer's flexibility. The following philosophies will be incorporated in the designs: (1) The exploratory shaft will be designed to fulfill its intended purpose which is to characterize the salt site by subsurface testing; (2) the design will minimize any adverse impact which the facility may cause to the environment and any damage to the site if it should be found suitable for a repository; (3) the health and safety of the public and of the workers will be an essential factor in the design; (4) sound engineering principles and practices will be consistently employed in the design process; (5) the exploratory shaft and related surface and subsurface facilities will be designed to be economical and reliable in construction, operation, and maintenance; and (6) the exploratory shaft facility will be designed in accordance with applicable federal, state, and local regulations, as well as all applicable national consensus codes and standards

Against the odds? De novo structure determination of a pilin with two cysteine residues by sulfur SAD.

Science.gov (United States)

Gorgel, Manuela; Bøggild, Andreas; Ulstrup, Jakob Jensen; Weiss, Manfred S; Müller, Uwe; Nissen, Poul; Boesen, Thomas

2015-05-01

Exploiting the anomalous signal of the intrinsic S atoms to phase a protein structure is advantageous, as ideally only a single well diffracting native crystal is required. However, sulfur is a weak anomalous scatterer at the typical wavelengths used for X-ray diffraction experiments, and therefore sulfur SAD data sets need to be recorded with a high multiplicity. In this study, the structure of a small pilin protein was determined by sulfur SAD despite several obstacles such as a low anomalous signal (a theoretical Bijvoet ratio of 0.9% at a wavelength of 1.8 Å), radiation damage-induced reduction of the cysteines and a multiplicity of only 5.5. The anomalous signal was improved by merging three data sets from different volumes of a single crystal, yielding a multiplicity of 17.5, and a sodium ion was added to the substructure of anomalous scatterers. In general, all data sets were balanced around the threshold values for a successful phasing strategy. In addition, a collection of statistics on structures from the PDB that were solved by sulfur SAD are presented and compared with the data. Looking at the quality indicator R(anom)/R(p.i.m.), an inconsistency in the documentation of the anomalous R factor is noted and reported.

Shaft flexibility effects on aeroelastic stability of a rotating bladed disk

Science.gov (United States)

Khader, Naim; Loewy, Robert

1989-01-01

A comprehensive study of Coriolis forces and shaft flexibility effects on the structural dynamics and aeroelastic stability of a rotating bladed-disk assembly attached to a cantilever, massless, flexible shaft is presented. Analyses were performed for an actual bladed-disk assembly, used as the first stage in the fan of the 'E3' engine. In the structural model, both in-plane and out-of-plane elastic deformation of the bladed-disk assembly were considered relative to their hub, in addition to rigid disk translations and rotations introduced by shaft flexibility. Besides structural coupling between blades (through the flexible disk), additional coupling is introduced through quasisteady aerodynamic loads. Rotational effects are accounted for throughout the work, and some mode shapes for the whole structure are presented at a selected rpm.

The effects of ascorbic acid on diphtheria toxin and intoxicated hela cells

International Nuclear Information System (INIS)

Clark, C.E.; Smith, T.J.

1976-01-01

Ascorbic acid (vitamin C) prevented diphtheria toxin from inhibiting the incorporation of [U- 14 C]-alanine into trichloroacetic acid precipitable material in HeLa cells. Ascorbic acid did not exhibit an effect on the adenosine diphosphate-ribosylation of amino acyl transferase 2 nor did it separate fragment A from fragment B in ''nicked'' toxin. A non-specific reducing agent, para-methylaminophenol sulfate, exhibited an effect of HeLa cells very similar to the results of ascorbic acid. Citric acid, a tricarboxylic acid, had no effect on HeLa cells. (auth.)

Numerical Simulation of Galvanic Corrosion Caused by Shaft Grounding Systems in Steel Ship Hulls

National Research Council Canada - National Science Library

Wang, Y

2005-01-01

The shaft grounding systems used on board HMC ships have substantially reduced the shaft-to-hull resistance and, thus, improved the performance of the shipboard impressed current cathodic protection (ICCP) system...

Dmp1 Promoter-Driven Diphtheria Toxin Receptor Transgene Expression Directs Unforeseen Effects in Multiple Tissues

Directory of Open Access Journals (Sweden)

Ahmed Al-Jazzar

2016-12-01

Full Text Available Mice harbouring a dentin matrix protein 1 (Dmp1 promoter-driven human diphtheria toxin (DT receptor (HDTR transgene (Tg have recently been used to attain targeted ablation of osteocytes by diphtheria toxin (DT treatment in order to define osteocyte function. Use of these Tg mice has asserted mechano- and novel paracrine regulatory osteocyte functions. To explore osteocyte roles fully, we sought to confirm the selectivity of DT effects in these transgenic mice. However, our findings revealed incomplete DT-induced osteocyte ablation, prevalent HDTR misexpression, as well as more prominent histopathological DT-induced changes in multiple organs in Tg than in wild-type (WT littermate mice. Mechanistic evidence for DT action, via prominent regulation of phosphorylation status of elongation factor-2 (EF-2, was also found in many non-skeletal tissues in Tg mice; indicative of direct “off-target” DT action. Finally, very rapid deterioration in health and welfare status in response to DT treatment was observed in these Tg when compared to WT control mice. Together, these data lead us to conclude that alternative models for osteocyte ablation should be sought and caution be exercised when drawing conclusions from experiments using these Tg mice alone.

The effect of induction motor shaft diameter on motor performance

Directory of Open Access Journals (Sweden)

Asım Gökhan Yetgin

2017-10-01

Full Text Available Induction motors are used in many areas from the past to the present and in different fields with the development of technology has continued to be used. It is obvious that induction motors as an improvement to the efficiency in terms of energy saving would cause great benefit. In that context, induction motor manufacturers and designers are constantly trying out new methods to improve motor performance and efficiency. In this study, what would be the optimum diameter of the shaft in order to increase the efficiency of the induction motor were investigated. In the study, 5.5 kW, 7.5 kW and 11 kW motors analyzes were also performed. Obtained shaft diameter values were compared with the manufacturer values. In addition, critical points such as the magnetic flux values, weight values and performances of the motors were examined and optimal shaft diameter values for each motor have been determined.

Reclamation of derelict land: procedure for locating abandoned mine shafts

Energy Technology Data Exchange (ETDEWEB)

1976-01-01

A procedure for locating abandoned shafts has been compiled from the experiences of those familiar with the problem. The procedure begins with a careful study of all the maps, aerial photographs and documents related to the mining activity and may include specialized surveys using geophysical, geochemical and aerial photographic methods when specific conditions are known or are likely to exist at the site. Direct methods, of either excavation, probing or drilling are required in each instance to confirm the location. Most of the methods are illustrated with case histories, and seismic and remote sensing methods are discussed in detail in appendices. Also in appendices, specific sources of information relating to mining are listed. Physical characteristics of mine shafts which are likely to have a bearing on the finding of the shaft are discussed, and an outline of the costs of the methods is presented. A glossary of mining terms used in the document and a detailed bibliography are provided.

High Specific Stiffness Shafts and Advanced Bearing Coatings for Gas Turbine Engines Final Report CRADA No. TC-1089-95

Energy Technology Data Exchange (ETDEWEB)

Barbee, Troy [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Chin, Herbert [United Technologies Corporation, East Hartford, CT (United States)

2017-11-09

At the time of the CRADA, the largest in-service gas-turbine aircraft engines strove for increased thrust and power density to meet the requirements for take-off thrust, given the increase in take-off gross weight (TOGW) associated with longer range transport requirements. The trend in modem turbo shaft engines was toward turbine shafts with higher and higher length-to-diameter ratios, which reduced the shaft critical speed. Using co nventional shaft materials, this lead to shafts that needed to operate near or above sensitive shaft bending critical speeds, therefore requiring multiple bearings and/ or multiple squeeze-film dampers to control the dynamic response. Using new materials and d esign concepts this project demonstrated the use of new shaft materials which could provide increased shaft speed range above existing maximum engine speeds without encountering a critic al speed event and high vector deflections. This increased main shaft speed also resulted in decreased bearing life associated with lower heat dissipation and higher centrifugal forces. Thus, a limited effort was devoted to feasibility of higher performance bearing coatings to mitigate the speed effects.

APPLICATION OF ELECTRONIC TACHEOMETER FOR PREAND POST-CONSTRUCTION SURVEY OF ELEVATOR SHAFT

Directory of Open Access Journals (Sweden)

M. S. Nesterionok

2015-01-01

Full Text Available Floor-by-floor survey of installed elements of an elevator shaft using coordinate method and tacheometer is also inhibited by the necessity to determine device installation points for floors of every mounting horizon that leads to additional measurements due to conditions of the completed building framework and presence of a great number of situational disturbances and then it entails significant time expenditure and diminution of accuracy in the obtained results. Technical capabilities of the modern electronic tacheometer substantially simplify an accomplishment of the mission because the device can be applied not only in function of vertical projection but it can be used for preand post-construction survey of the elevator shaft with the help of advanced radiation method. While using the given method the electronic tacheometer equipped with diagonal ocular is installed over a bearing sign in the elevator pit and control points of the elevator shaft are determined with the help of specially developed portable beam deflector in the form of disc with radius r, which is fixed to a handle rod and orientated perpendicularly to a laser beam. An innovation diagram for preand post-construction survey of elevator shaft has been developed while applying this type of deflector. The diagram is characterized by high efficiency due to the fact that one tacheometer installation makes it possible to execute highly accurate, complete plane and high-level surveying of the whole elevator shaft including doorways, door sills and jambs.The paper proposes a new method for pre and post construction survey of the elevator shaft using the electronic tacheometer while applying specially developed beam deflector that ensures a geometric fixing of one or simultaneously two surveying points to spatial coordinate system where the preand post-construction survey is carried out. The method is characterized by high geometric accuracy and technological efficiency.

Measuring surface-water loss in Honouliuli Stream near the ‘Ewa Shaft, O‘ahu, Hawai‘i

Science.gov (United States)

Rosa, Sarah N.

2017-05-30

The Honolulu Board of Water Supply is currently concerned with the possibility of bacteria in the pumped water of the ‘Ewa Shaft (State well 3-2202-21). Groundwater from the ‘Ewa Shaft could potentially be used to meet future potable water needs in the ‘Ewa area on the island of O‘ahu. The source of the bacteria in the pumped water is unknown, although previous studies indicate that surface water may be lost to the subsurface near the site. The ‘Ewa Shaft consists of a vertical shaft, started near the south bank of Honouliuli Stream at an altitude of about 161 feet, and two horizontal infiltration tunnels near sea level. The shaft extracts groundwater from near the top of the freshwater lens in the Waipahu-Waiawa aquifer system within the greater Pearl Harbor Aquifer Sector, a designated Water Management Area.The surface-water losses were evaluated with continuous groundwater-level data from the ‘Ewa Shaft and a nearby monitoring well, continuous stream-discharge data from U.S. Geological Survey streamflow-gaging station 16212490 (Honouliuli Stream at H-1 Freeway near Waipahu), and seepage-run measurements in Honouliuli Stream and its tributary. During storms, discharge at the Honouliuli Stream gaging station increases and groundwater levels at ‘Ewa Shaft and a nearby monitoring well also increase. The concurrent increase in water levels at ‘Ewa Shaft and the nearby monitoring well during storms indicates that regional groundwater-level changes related to increased recharge, reduced withdrawals (due to a decrease in demand during periods of rainfall), or both may be occurring; although these data do not preclude the possibility of local recharge from Honouliuli Stream. Discharge measurements from two seepage runs indicate that surface water in the immediate area adjacent to ‘Ewa Shaft infiltrates into the streambed and may later reach the groundwater system developed by the ‘Ewa Shaft. The estimated seepage loss rates in the vicinity of �

Immunity to tetanus and diphtheria in rural Africa

DEFF Research Database (Denmark)

Kurtzhals, J A; Kjeldsen, K; Hey, A S

1997-01-01

To assess the effect of the Expanded Program on Immunization (EPI) in rural Africa, blood samples were collected in two Kenyan sublocations. Serum antibodies against tetanus toxoid were measured in 155 individuals 1-70 years of age. Titers greater than the protective level of 0.01 IU/ml were found...... in 47% of the population. Protection was significantly higher in children born after the launching of the EPI (68%) and in women who had been at childbearing age since then (69%). Significantly lower protection was demonstrated in other age and sex-groups. The level of protection in children was equal...... in the two populations, whereas protection in fertile women was significantly lower in the population living a long distance from a health center. Diphtheria anti-toxin was measured in the samples from one sublocation, and 70 of 84 individuals (83%) had antibody levels greater than the protective level...

Viscoelastic Characterization of Long-Eared Owl Flight Feather Shaft and the Damping Ability Analysis

Directory of Open Access Journals (Sweden)

Jia-li Gao

2014-01-01

Full Text Available Flight feather shaft of long-eared owl is characterized by a three-parameter model for linear viscoelastic solids to reveal its damping ability. Uniaxial tensile tests of the long-eared owl, pigeon, and golden eagle flight feather shaft specimens were carried out based on Instron 3345 single column material testing system, respectively, and viscoelastic response of their stress and strain was described by the standard linear solid model. Parameter fitting result obtained from the tensile tests shows that there is no significant difference in instantaneous elastic modulus for the three birds’ feather shafts, but the owl shaft has the highest viscosity, implying more obvious viscoelastic performance. Dynamic mechanical property was characterized based on the tensile testing results. Loss factor (tanδ of the owl flight feather shaft was calculated to be 1.609 ± 0.238, far greater than those of the pigeon (0.896 ± 0.082 and golden eagle (1.087 ± 0.074. It is concluded that the long-eared owl flight feather has more outstanding damping ability compared to the pigeon and golden eagle flight feather shaft. Consequently, the long-eared owl flight feathers can dissipate the vibration energy more effectively during the flying process based on the principle of damping mechanism, for the purpose of vibration attenuation and structure radiated noise reduction.

Development of the laser alignment system with PSD used for shaft calibration

Science.gov (United States)

Jiao, Guohua; Li, Yulin; Hu, Baowen

2006-02-01

Shaft calibration is an important technique during installation and maintenance of a rotating machine. It requires unique and high-precision measurement instruments with calculation capability, and relies on experience on heavy, high-speed, or high-temperature machines. A high-precision laser alignment system has been designed using PSD (Position Sensing Detector) to change traditional manual way of shaft calibration and to make the measurement easier and more accurate. The system is comprised of two small measuring units (Laser transmitter and detector) and a hand operated control unit or a PC. Such a laser alignment system has been used in some actual shaft alignment with offset resolution 1.5μm and angular resolution 0.1°.

The WHO Review of the Possible Nonspecific Effects of Diphtheria-Tetanus-Pertussis Vaccine

DEFF Research Database (Denmark)

Aaby, Peter; Ravn, Henrik; Benn, Christine S

2016-01-01

BACKGROUND: World Health Organization recently reviewed the possible nonspecific effects of diphtheria-tetanus-pertussis (DTP) vaccine. The results were considered inconsistent though most studies suggested deleterious effects. We examined whether inconsistencies in results reflected differences...... in effect of DTP or differences in the methodology used in different studies. METHODS: If children remain unvaccinated because they are frail or if children (including dead ones) with no information on vaccination status are classified as "unvaccinated," the mortality rate becomes unnaturally high among...

Diphtheria, tetanus, poliomyelitis, yellow fever and hepatitis B seroprevalence among HIV1-infected migrants. Results from the ANRS VIHVO vaccine sub-study.

Science.gov (United States)

Mullaert, Jimmy; Abgrall, Sophie; Lele, Nathalie; Batteux, Frederic; Slama, Lilia Ben; Meritet, Jean-Francois; Lebon, Pierre; Bouchaud, Olivier; Grabar, Sophie; Launay, Odile

2015-09-11

Few data are available on the seroprotection status of HIV1-infected patients with respect to vaccine-preventable diseases. To describe, in a population of HIV1-infected migrants on stable, effective ART therapy, the seroprevalence of diphtheria, poliomyelitis, tetanus, yellow fever antibodies and serostatus for hepatitis B, and to identify factors associated with seroprotection. Vaccine responses against diphtheria, tetanus, poliomyelitis and yellow fever were also studied. Sub-Saharan African patients participating in the ANRS-VIHVO cohort were enrolled prior to travel to their countries of origin. Serologic analyses were performed in a central laboratory before and after the trip. Univariate and multivariate logistic regression was used to identify factors associated with initial seroprotection. 250 patients (99 men and 151 women) were included in the seroprevalence study. Median age was 45 years (IQR 39-52), median CD4 cell count was 440/μL (IQR 336-571), and 237 patients (95%) had undetectable HIV1 viral load. The initial seroprevalence rates were 69.0% (95%CI 63.2-74.7) for diphtheria, 70.7% (95%CI 65.0-76.3) for tetanus, and 85.9% (95%CI 81.6-90.2) for yellow fever. Only 64.4% (95%CI 58.5-70.3) of patients had protective antibody titers against all three poliomyelitis vaccine strains before travel. No serological markers of hepatitis B were found in 18.6% of patients (95%CI 13.7-23.3). Patient declaration of prior vaccination was the only factor consistently associated with initial seroprotection. We found a low prevalence of seroprotection against diphtheria, poliomyelitis, tetanus and hepatitis B. HIV infected migrants living in France and traveling to their native countries need to have their vaccine schedule completed. Copyright © 2015 Elsevier Ltd. All rights reserved.

Explosion-proof conservation of the Lohberg shaft 1 and 2; Explosionsfeste Konservierung der Schaechte Lohberg 1 und 2

Energy Technology Data Exchange (ETDEWEB)

Hachmann, Andreas [AHA - Andreas Hachmann, Recklinghausen (Germany). Pruefung und Abnahme

2010-03-15

In the course of closure of the former Lohberg/Osterfeld colliery backfilling of shafts 1 and 2 with concrete was envisaged according to the original plans. On the basis of a modified plan, however, the two shafts will now be maintained for drainage. Hence a suspended pack was constructed on the 3{sup rd} level for each of the two shafts. Both shafts must be explosion-proof until the water is raised. The structural steel solution described in more detail in this contribution was applied in both shafts. (orig.)

Monitoring device for shaft oscillation of reactor incorporated-type recycling pump

International Nuclear Information System (INIS)

Miyashita, Kaoru; Shibasaki, Kimiyuki.

1995-01-01

The present invention concerns monitoring of recycling pump shaft oscillation in a BWR type reactor, which monitors by separating a rotation pulse signal and a shaft oscillation waveform signal obtained in a non-contact type displacement meter. Namely, a threshold value calculation means of a separation processing section takes in original waveform data and selects the maximum value and the minimum value among them. A threshold value is calculated based on the values. An average value of the original waveform data for portions which do not exceed the threshold value is calculated. A first calculation means compares each of the original data with the threshold value, and if the original data are greater than the threshold value, they are outputted as the original data corresponding to the rotation pulse signal. When the original data are smaller than the threshold value, they are outputted as they are as a shaft oscillation waveform signal. On the other hand, a second calculation means calculates an average value for the pulse of the original waveform data corresponding to the rotation pulse signal. An average value of the original waveform data which do not exceed the threshold value are subtracted from the average value, to form the shaft oscillation waveform signal and output the same. (I.S.)

Exploratory shaft facility preliminary designs - Permian Basin

International Nuclear Information System (INIS)

1983-09-01

The purpose of the Preliminary Design Report, Permian Basin, is to provide a description of the preliminary design for an Exploratory Shaft Facility in the Permian Basin, Texas. This issue of the report describes the preliminary design for constructing the exploratory shaft using the Large Hole Drilling method of construction and outlines the preliminary design and estimates of probable construction cost. The Preliminary Design Report is prepared to complement and summarize other documents that comprise the design at the preliminary stage of completion, December 1982. Other design documents include drawings, cost estimates and schedules. The preliminary design drawing package, which includes the construction schedule drawing, depicts the descriptions in this report. For reference, a list of the drawing titles and corresponding numbers are included in the Appendix. The report is divided into three principal sections: Design Basis, Facility Description, and Construction Cost Estimate. 30 references, 13 tables

Reliability of agriculture universal joint shafts based on temperature measuring in universal joint bearing assemblies

Directory of Open Access Journals (Sweden)

Аleksandar Asonja

2015-03-01

Full Text Available This paper presents a research into reliability calculations of agriculture double universal joint shafts based on temperature measuring in cardan-type universal joint bearing assemblies. Special laboratory equipment was developed for this research which is presented in the paper. The objective of this research was to test the real life span of universal joint shafts in the laboratory and in field, to obtain the results which can be used to improve the reliability of universal joint shafts. If the presented research were used along with maintenance measures recommended in the paper and with proper use, the level of reliability of the shafts would be 2.1 times higher. The presented results of the research showed that needle bearings, i.e. bearing assemblies of the joints, are the most critical elements on universal joint shafts and are possible causes of their lower reliability. The second universal joint is the part with the lowest reliability in the observed technical system.

Experimental and numerical study of the effect of rolling parameters on shaft deformation during the longitudinal rolling process

Science.gov (United States)

Kowalik, Marek; Trzepiecinski, Tomasz

2018-05-01

This paper presents the characteristics of the process of longitudinal rolling of shafts and the geometry of the working section of forming rollers with a secant profile. In addition, the analytical formulae defining the geometry of a roller profile were determined. The experiments were carried out on shafts made of S235JR and C45 structural steels and the MSC.Marc + Mentat program was used for the numerical analysis of the rolling process based on the finite element method. The paper analyses the effect of roller geometry on the changes in value of the widening coefficient and the diameter reduction coefficient for the first forming passage. It was found that the mechanical properties of the shaft material have a slight influence on the widening coefficient. The value of the widening coefficient of the shaft increases with increase in the initial diameter of the shaft. Increasing shaft diameter causes an increase of strain gradient on the cross-section of the shaft.

Scanning Electron Microscopic Hair Shaft Analysis in Ectodermal Dysplasia Syndromes.

Science.gov (United States)

Hirano-Ali, Stefanie A; Reed, Ashley M; Rowan, Brandon J; Sorrells, Timothy; Williams, Judith V; Pariser, David M; Hood, Antoinette F; Salkey, Kimberly

2015-01-01

The objective of the current study was to catalog hair shaft abnormalities in individuals with ectodermal dysplasia (ED) syndromes using scanning electron microscopy (SEM) and to compare the findings with those in unaffected controls. This is the second of a two-part study, the first of which used light microscopy as the modality and was previously published. Scanning electron microscopy was performed in a blinded manner on hair shafts from 65 subjects with seven types of ED syndromes and 41 unaffected control subjects. Assessment was performed along the length of the shaft and in cross section. Hair donations were collected at the 28th Annual National Family Conference held by the National Foundation for Ectodermal Dysplasia. Control subjects were recruited from a private dermatology practice and an academic children's hospital outpatient dermatology clinic. SEM identified various pathologic hair shaft abnormalities in each type of ED and in control patients. When hairs with all types of ED were grouped together and compared with those of control patients, the difference in the presence of small diameter and shallow and deep grooves was statistically significant (p < 0.05). When the EDs were separated according to subtype, statistically significant findings were also seen. SEM is a possible adjuvant tool in the diagnosis of ED syndromes. There are significant differences, with high specificity, between the hairs of individuals with ED and those of control subjects and between subtypes. © 2015 Wiley Periodicals, Inc.

Examination of reactor coolant pump shaft at Crystal River-3

International Nuclear Information System (INIS)

Hayner, G.O.; Frye, C.R.; Clary, M.D.

1987-01-01

A detailed examination was performed on a broken RCP shaft assembly. The primary fracture was located in a groove under the upper end of the hydrostatic bearing journal. Additionally, all four impeller-to-shaft bolts and one drive pin failed. Mechanical properties, bulk chemistry, hardness, and microstructure were normal for the A-286 material used for the shaft, bolts and pins. A zone of axial surface cracking was seen just above the top of the hydrostatic bearing journal. According to Yoon et.al. these cracks are caused by thermal fatigue resulting from turbulent hot and cold water mixing in this area and have a self limiting depth. The primary RCP shaft fracture was caused by high cycle fatigue. Crack initiation probably occurred during initial use of the RCP and according to Yoon was caused by a combination postulated effect of comined surface residual stresses and stress concentration in the groove area. Several combinations of effects including broken impeller bolts probably were responsible for the initial crack propagation. Fracture mechanics testing results in 550 0 F air and simulated PWR water were used to estimate the stress intensity range of the primary crack and the crack propagation time by comparison of the fracture surface features. These estimates indicated that the propagation time was probably in the range from ≅ 191 to ≅ 323 days with a maximum stress intensity level of ≅ 30 ksi √(in). (orig.)

Three-Dimensional Numerical Analysis of Compound Lining in Complex Underground Surge-Shaft Structure

Directory of Open Access Journals (Sweden)

Juntao Chen

2015-01-01

Full Text Available The mechanical behavior of lining structure of deep-embedded cylinder surge shaft with multifork tunnel is analyzed using three-dimensional nonlinear FEM. With the elastic-plastic constitutive relations of rock mass imported and the implicit bolt element and distributed concrete cracking model adopted, a computing method of complex surge shaft is presented for the simulation of underground excavations and concrete lining cracks. In order to reflect the interaction and initial gap between rock mass and concrete lining, a three-dimensional nonlinear interface element is adopted, which can take into account both the normal and tangential characteristics. By an actual engineering computation, the distortion characteristics and stress distribution rules of the dimensional multifork surge-shaft lining structure under different behavior are revealed. The results verify the rationality and feasibility of this computation model and method and provide a new idea and reference for the complex surge-shaft design and construction.

Reactor coolant pump shaft seal behavior during blackout conditions

International Nuclear Information System (INIS)

Mings, W.J.

1985-01-01

The United States Nuclear Regulatory Commission has classified the problem of reactor coolant pump seal failures as an unresolved safety issue. This decision was made in large part due to experimental results obtained from a research program developed to study shaft seal performance during station blackout and reported in this paper. Testing and analysis indicated a potential for pump seal failure under postulated blackout conditions leading to a loss of primary coolant with a concomitant danger of core uncovery. The work to date has not answered all the concerns regarding shaft seal failure but it has helped scope the problem and focus future research needed to completely resolve this issue

TMI-2 auxiliary building elevator shaft and pit decontamination

Energy Technology Data Exchange (ETDEWEB)

Bengel, T.G.

1986-01-01

Decontamination of the elevator pit and shaft in the auxiliary building at Three Mile Island Unit 2 (TMI-2) was performed to remove high radiation and contamination levels which prevented personnel from utilizing the elevator. The radiation and contamination levels in the TMI-2 auxiliary building elevator shaft have been reduced to the point where plant personnel are again permitted to ride in the elevator without a radiation work permit, with the exception of access to the 281-ft (basement) level. Based on the declassification and expanded use of the elevator, the task goal has been met. The tax expended 16.16 man-rem and 621 man-hours.

Numerical Study on the Tensile Behavior of 3D Four Directional Cylindrical Braided Composite Shafts

Science.gov (United States)

Zhao, Guoqi; Wang, Jiayi; Hao, Wenfeng; Liu, Yinghua; Luo, Ying

2017-10-01

The tensile behavior of 3D four directional cylindrical braided composite shafts was analyzed with the numerical method. The unit cell models for the 3D four directional cylindrical braided composite shafts with various braiding angles were constructed with ABAQUS. Hashin's failure criterion was used to analyze the tensile strength and the damage evolution of the unit cells. The influence of the braiding angle on the tensile behavior of the 3D four directional cylindrical braided composite shafts was analyzed. The numerical results showed that the tensile strength along the braiding direction increased as the braiding angle decreased. These results should play an integral role in the design of braiding composites shafts.

Buoyancy and Pressure Driven Flow of Hot Gases in Vertical Shafts with Natural and Forced Ventilation

Science.gov (United States)

Tamm, Gunnar; Jaluria, Yogesh

2003-11-01

An experimental investigation has been carried out on the buoyancy and pressure induced flow of hot gases in vertical shafts, in order to simulate the propagation of combustion products in elevator shafts due to fire in multilevel buildings. Various geometrical configurations are studied, with regard to natural and forced ventilation imposed at the top or bottom of the vertical shaft. The aspect ratio is taken at a fixed value of 6 and the inflow conditions for the hot gases, at a vent near the bottom, are varied in terms of the Reynolds and Grashof numbers. Temperature measurements within the shaft allow a detailed study of the steady state thermal fields, from which optimal means for smoke alleviation in high-rise building fires may be developed. Flow visualization is also used to study the flow characteristics. The results obtained indicate a wall plume as the primary transport mechanism. Flow recirculation dominates at high Grashof number flows, while increased Reynolds numbers gives rise to greater mixing in the shaft. The development and stability of the flow and its effect on the spread of smoke and hot gases are assessed for the different shaft configurations and inlet conditions. It is found that the fastest smoke removal and lowest shaft temperatures occur for a configuration with natural ventilation at the top and forced ventilation up from the shaft bottom. It is also shown that forced ventilation can be used to arrest smoke spread, as well as to dilute the effects of the fire.

Site study plan for exploratory shaft monitoring wells, Deaf Smith County Site, Texas: Preliminary Draft

International Nuclear Information System (INIS)

1988-01-01

As part of site characterization studies, two exploratory shafts will be constructed at the Deaf Smith County site, Texas. Twelve wells at five locations have been proposed to monitor potential impacts of shaft construction on water-bearing zones in the Ogallala Formation and the Dockum Group. In addition, tests have been proposed to determine the hydraulic properties of the water-bearing zones for use in design and construction of the shafts. Samples of the Blackwater Draw Formation, Ogallala Formation, and Dockum Group will be obtained during construction of these wells. Visual indentification, laboratory testing, and in situ testing will yield data necessary for Exploratory Shaft Facility design and construction. This activity provides the earliest data on the Blackwater Drew Formation, Ogallala Formation, and Dockum Group near the exploratory shaft locations. Drilling and hydrologic testing are scheduled prior to other subsurface activity at the Exploratory Shaft Facility to establish ground-water baseline conditions. The Technical Field Services Contractor is responsible for conducting the field program of drilling and testing. Samples and data will be handled and reported in accordance with established Salt Repository Project procedures. A quality assurance program will be utilized to assure that activities affecting quality are performed correctly and that the appropriate documentation is maintained. 45 refs., 11 figs., 4 tabs

Torsional analysis of 1 MW gearbox and shaft system in the Avedoere wind turbine

International Nuclear Information System (INIS)

Crone, A.

1993-05-01

In order to predict undesired high gear noise emission from the Avedoere wind turbine due to critical torsional resonances in the shaft system, the torsional natural frequencies and corresponding mode shapes have been calculated. The analysis has involved a comprehensive, detailed model of the gearbox shaft system. The natural frequencies and mode shapes have also been calculated for the test bed shaft system enabling a calibration of the calculations when comparing with measurements made on the test bed system. The natural torsional frequencies of the test bed shaft system and the wind turbine shaft system, both including the Flender, Peak 4375 gearbox, have been calculated together with the corresponding mode shapes. The sensitivity analysis showed that the natural frequencies of one of the torsional modes may be close to or coinciding with the toothmesh frequency of the output gear stage, in the wind turbine shaft system. The shape of this mode indicates however, that this mode is not very likely to be strongly excited by forces acting in the tooth contact at the mesh frequency. This conclusion also counts for a second mode which was calculated to have a natural frequency close to the toothmesh frequency of the output stage. The analysis also shows that the frequencies of the 1st, the 3rd and the 4th harmonics of the toothmesh frequency of the 2nd gear stage, deviate by less than 15% from several modes of the wind turbine shaft system, which seem likely to be excited by forces acting in the tooth contact of this stage. Amplification of the structure-borne noise from the gearbox at these frequencies may be expected. The amplification at these frequencies is not expected to have any critical influence on the tonal gear noise radiated from the wind turbine. (EG)

Results from Investigations of Torsional Vibration in Turbine Set Shaft Systems

Science.gov (United States)

Taradai, D. V.; Deomidova, Yu. A.; Zile, A. Z.; Tomashevskii, S. B.

2018-01-01

The article generalizes the results obtained from investigations of torsional vibration in the shaft system of the T-175/210-12.8 turbine set installed at the Omsk CHPP-5 combined heat and power plant. Three different experimental methods were used to determine the lowest natural frequencies of torsional vibration excited in the shaft system when the barring gear is switched into operation, when the generator is synchronized with the grid, and in response to unsteady disturbances caused by the grid and by the turbine control and steam admission system. It is pointed out that the experimental values of the lowest natural frequencies (to the fourth one inclusively) determined using three different methods were found to be almost completely identical with one another, even though the shaft system was stopped in the experiments carried out according to one method and the shaft system rotated at the nominal speed in those carried out according to two other methods. The need to further develop the experimental methods for determining the highest natural frequencies is substantiated. The values of decrements for the first, third, and fourth natural torsional vibration modes are obtained. A conclusion is drawn from a comparison between the calculated and experimental data on the shaft system's static twisting about the need to improve the mathematical models for calculating torsional vibration. The measurement procedure is described, and the specific features pertinent to the way in which torsional vibration manifests itself as a function of time and turbine set operating mode under the conditions of its long-term operation are considered. The fundamental measurement errors are analyzed, and their influence on the validity of measured parameters is evaluated. With an insignificant level of free and forced torsional vibrations set up under the normal conditions of turbine set and grid operation, it becomes possible to exclude this phenomenon from the list of main factors

The Introduction of Diphtheria-Tetanus-Pertussis and Oral Polio Vaccine Among Young Infants in an Urban African Community

DEFF Research Database (Denmark)

Mogensen, Søren Wengel; Andersen, Andreas; Rodrigues, Amabelia

2017-01-01

Background We examined the introduction of diphtheria-tetanus-pertussis (DTP) and oral polio vaccine (OPV) in an urban community in Guinea-Bissau in the early 1980s. Methods The child population had been followed with 3-monthly nutritional weighing sessions since 1978. From June 1981 DTP and OPV...

Numerical analysis on the action of centrifuge force in magnetic fluid rotating shaft seals

Science.gov (United States)

Zou, Jibin; Li, Xuehui; Lu, Yongping; Hu, Jianhui

2002-11-01

The magnetic fluid seal is suitable for high-speed rotating shaft seal applications. Centrifuge force will have evident influence on magnetic fluid rotating shaft seals. The seal capacity of the rotating shaft seal can be improved or increased by some measures. Through hydrodynamic analysis the moving status of the magnetic fluid is worked out. By numerical method, the magnetic field and the isobars in the magnetic fluid of a seal device are computed. Then the influence of the centrifuge force on the magnetic fluid seal is calculated quantitatively.

Numerical analysis on the action of centrifuge force in magnetic fluid rotating shaft seals

International Nuclear Information System (INIS)

Zou Jibin; Li Xuehui; Lu Yongping; Hu Jianhui

2002-01-01

The magnetic fluid seal is suitable for high-speed rotating shaft seal applications. Centrifuge force will have evident influence on magnetic fluid rotating shaft seals. The seal capacity of the rotating shaft seal can be improved or increased by some measures. Through hydrodynamic analysis the moving status of the magnetic fluid is worked out. By numerical method, the magnetic field and the isobars in the magnetic fluid of a seal device are computed. Then the influence of the centrifuge force on the magnetic fluid seal is calculated quantitatively

Vertically loaded pivot bearing for high-speed shafts

International Nuclear Information System (INIS)

Zippe, G.; Werner, D.; Keller, A.; Stephan, E.

1974-01-01

The pivot bearing subjected to vertical loads has a pivot with a rounded end and a diametral slot and a cup-shaped recess in the bearing box. The end of the pivot and the recess constitute a gap which widens towards the periphery of the bearing. The bearing box carries an attachment with a clearance, the shape of a hollow cylinder and accomodating the pivot and its shaft. To feed the lubricating oil surrounding and filling the bearing there is a groove either in the wall of the clearance or at the end of the pivot in the direction of rotation of the shaft or against it. The oil carried to the support point flows back into the oil bath surrounding the bearing through a slot in the bearing box. (DG) [de

Balancing of a power-transmission shaft with the application of axial torque

Science.gov (United States)

Zorzi, E. S.; Flemming, D.

1980-01-01

Evaluation of power transmission shafting for high-speed balancing has shown that when axial torque is applied, the imbalance response is altered. An increase in synchronous excitation always occurs if the axial torque level is altered from the value used during balancing; this was the case even when the shaft was balanced with torque applied. The twisting of the long slender shaft produces a change in the imbalance distribution sufficient to disrupt the balanced state. This paper presents a review of the analytic development of a weighted least squares approach to influence coefficient balancing and a review of experimental results. The analytic approach takes advantage of the fact that the past testing has shown that the influence coefficients are not significantly affected by the application of axial torque. The 3.60-m (12-ft) long aluminum shaft, 7.62 cm (3 in.) in diameter was run through the first flexural critical speed at torque levels ranging from zero-torque to 903.8 N-M (8000 lb-in.) in 112.9 N-M (1000 lb-in.) increments. Good comparison was achieved between predicted and experimental results.

Simulation Analysis of Spherical Mechanical Seal Property of Marine Stern Shaft

OpenAIRE

Zhou Xu Hui; Zou Li

2016-01-01

The finite element model of spherical mechanical seal wasestablished with ANSYS, and the influence of seawater pressure, shaft speed and other factors on the sealing performance was discussed. The study results show that local contact situation of the spherical mechanical seal is in the outside of the seal rings, and both maximum contact pressure and temperature appearat the same position. As sea water pressure and stern shaft rotary speed are increased, the contact pressure and temperature o...

30 CFR 57.4261 - Shaft-station waterlines.

Science.gov (United States)

2010-07-01

....4261 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE SAFETY AND HEALTH SAFETY AND HEALTH STANDARDS-UNDERGROUND METAL AND NONMETAL MINES Fire Prevention... located at underground shaft stations and are part of the mine's fire protection system shall have at...

Safety and immunogenicity of tetanus-diphtheria-acellular pertussis vaccine administered to children 10 or 11 years of age.

Science.gov (United States)

Marshall, Gary S; Pool, Vitali; Greenberg, David P; Johnson, David R; Sheng, Xiaohua; Decker, Michael D

2014-11-01

Boosting immunity to tetanus, diphtheria, and pertussis through the use of Tdap vaccines is routinely recommended at 11 to 12 years of age; some states, however, require Tdap for entry into middle school, which may begin at 10 years of age. This study was conducted to determine whether Tdap5 (Adacel), which is licensed for use in children beginning at 11 years of age, is as safe and immunogenic in 10-year-olds as it is in 11-year-olds. Children who had received 5 previous doses of any diphtheria-tetanus-acellular pertussis (DTaP) vaccine were enrolled in a phase IV clinical trial; 646 10-year-olds and 645 11-year-olds completed the study, which involved a single intramuscular dose of Tdap5 along with pre- and postvaccination serologies. Postvaccination geometric mean concentrations (GMCs) of antibody to pertussis antigens (pertussis toxoid, filamentous hemagglutinin, pertactin, and fimbria types 2 and 3) of 10-year-olds were noninferior to those of 11-year-olds, as were booster response rates for all pertussis antibodies, except for those to fimbrial antigens (94% and 97%, respectively). Seroprotection rates among 10-year-olds for tetanus and diphtheria were noninferior to those in 11-year-olds. Rates of injection site reactions, solicited systemic reactions, and unsolicited adverse events, adverse reactions, and serious adverse events were similar in the two groups. These data support the conclusion that Tdap5 is safe and immunogenic in 10-year-olds. (This study has been registered at ClinicalTrials.gov under registration no. NCT01311557.). Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Outcome of non-operative management of femoral shaft fractures in children

Directory of Open Access Journals (Sweden)

Akinyoola A

2011-01-01

Full Text Available Background: Femoral shaft fractures are common injuries in childhood. There is paucity of information on their presentation and outcome of the available treatment methods in the African population. This study evaluated the outcome of non-operative methods of treatment of femoral shaft fractures in our centre. Patients and Methods: A retrospective review of the database of children aged 14 years and below with femoral shaft fractures treated non-operatively over a 10-year period. Results: A total of 134 patients with 138 fractures met the study criteria. This consisted of 71 boys (mean age = 6.1 years ± SD and 63 girls (mean age = 6.5 years ± SD. Pedestrian vehicular accident was the most common cause of femoral shaft fractures in the study population. The midshaft was the most common site of fractures. There were associated injuries to other parts of the body (especially head injury in 34.3% of the patients. The commonest mode of treatment was skin traction only (87.7%. The mean time to fracture union was 4.9 weeks ± SD (range = 3-15 weeks. The mean length of hospitalisation was 6.7 weeks ± SD (range = 5 days-11 weeks. There was a fairly strong positive correlation between the length of hospitalisation and the presence of associated injuries, especially head injury, upper limb fractures and bilaterality of the fractures. The mean total cost of treatment was #7685 (Naira or $51.2 (range = $14.2-$190. At the last follow up, 97.8% of the fractures united without significant angulation or shortening. Conclusion: The outcome of non-operative treatment of femoral shaft fractures in our setting is comparable to the results of other workers. Methods of treatment that shorten the length of hospitalisation without unduly increasing cost should be encouraged.

Concepts for backfilling and sealing of shafts

International Nuclear Information System (INIS)

Pierau, B.

1990-01-01

The disposal site is situated at a depth of 1000 to 1200 meters. It is covered by very thick cretatious mudstone layers forming the main barrier against the spread of radioactively contaminated water into the biosphere. Because of the excavation works and the resulting stress redistributions, the material surrounding the shafts is probably broken up, which leads to increased permeability in comparison with the intact rock. It is planned to backfill the shafts with an insoluble mineral mixture including a fine fraction necessary to achieve the sealing required. The joints and cracks in the brocken-up surrounding material are believed to be sealed by themselves due to swelling of the mudstone. Some strata of the mudstone contain more than 20% of smektite, a swelling clay mineral. Those regions, where the broken-up zone cannot be considered sure to self-seal due to swelling, are planned to be sealed by pressure grouting using clay suspension. (orig./HP) [de

Detection of cracks in shafts with the Approximated Entropy algorithm

Science.gov (United States)

Sampaio, Diego Luchesi; Nicoletti, Rodrigo

2016-05-01

The Approximate Entropy is a statistical calculus used primarily in the fields of Medicine, Biology, and Telecommunication for classifying and identifying complex signal data. In this work, an Approximate Entropy algorithm is used to detect cracks in a rotating shaft. The signals of the cracked shaft are obtained from numerical simulations of a de Laval rotor with breathing cracks modelled by the Fracture Mechanics. In this case, one analysed the vertical displacements of the rotor during run-up transients. The results show the feasibility of detecting cracks from 5% depth, irrespective of the unbalance of the rotating system and crack orientation in the shaft. The results also show that the algorithm can differentiate the occurrence of crack only, misalignment only, and crack + misalignment in the system. However, the algorithm is sensitive to intrinsic parameters p (number of data points in a sample vector) and f (fraction of the standard deviation that defines the minimum distance between two sample vectors), and good results are only obtained by appropriately choosing their values according to the sampling rate of the signal.

Sealing considerations for repository shafts in bedded and dome salt

International Nuclear Information System (INIS)

1981-12-01

The report reviews the geologic and hydrologic data base for penetration seal designs referenced to the Los Medanos bedded salt site in New Mexico and to four candidate salt domes in the Gulf Interior. Experience with existing shafts highlights the importance, for shaft decommissioning as well as operation, of achieving an adequate seal at and immediately below the top of salt. Possible construction procedures for repository shafts are reviewed, noting advantages and disadvantages with respect to repository sealing. At this stage, there does not appear to be a clear preference for excavation by drill and blast or by drilling. If conventional drill and blast methods are used, it may be necessary to grout in permeable zones above the salt. An important consideration with respect to sealing is that grouting operations (or freezing should it be used) should not establish connections between the top of salt and water-bearing zones higher in the stratigraphic section. Generally, it is concluded that Los Medanos and the dome salt sites are favorable candidate repository sites from the point of view of sealing

Compliance with diphtheria, tetanus, and pertussis immunisation in Bangladesh

DEFF Research Database (Denmark)

Zeitlyn, S; Rahman, A K; Nielsen, B H

1992-01-01

OBJECTIVE: To evaluate factors associated with non-compliance with having second vaccination against diphtheria, tetanus, and pertussis in a treatment centre in Dhaka to determine which children were most at risk of not completing immunisation. DESIGN: Cohort study of infants given first dose...... of the vaccine and followed up six weeks later to ascertain compliance with having second dose. Factors associated with non-compliance were evaluated. SETTING: Dhaka treatment centre of the International Centre for Diarrhoeal Disease Research, Bangladesh. SUBJECTS: 136 unimmunised children aged 6 weeks to 23...... of immunisation, and she was given clear instructions to bring the child back after four weeks for the second dose. MAIN OUTCOME MEASURE: Rate of non-compliance with advice to return child for second vaccination. RESULTS: 46 of 113 children (41%) received the second dose of the vaccine. Factors most closely...

Development of a new universal machine device for eccentric shafts processing

Directory of Open Access Journals (Sweden)

Михайло Володимирович Маргуліс

2015-11-01

Full Text Available The analysis of the existing lathe devices for machining of eccentric details and the reasons for the development of a new universal machine device– a shifting lathe center-have been described in the article. The device design, its operating principle, the main parts functions and elements of the case were described and illustrated One of the most complicated cases of eccentric shaft turning, that is turning design and scheme of the eccentric shaft of precessional harmonic drive with intermediate rolling bodies when the proposed device was used was described in the article. The shifting lathe center can reduce the complexity of the machine tool for turning eccentric shafts configuration. The ability to install the shifting center in the tailstock and headstock of the lathe, and the availability of the leash makes it possible to turn various eccentric parts, conical surfaces, to apply the device to compensate for the emerging taper in cylindrical shafts processing. All the above mentioned makes the device universal. The specific feature of this device is the use of a ball center and the connection of the centering element with the adjusting screw by a fine thread screw, this increasing the precision machining. The protective cover of the device makes it possible to reduce the chance of possible injury from protruding parts of the device, namely the leash and the centering element

Characterization of ultraviolet light-induced diphtheria toxin-resistant mutations in normal and Xeroderma pigmentosum human fibroblasts

International Nuclear Information System (INIS)

Glover, T.W.

1979-01-01

Quantitative mutagenesis studies in human cells have been severely limited by the lack of reliable genetic markers. Experiments were therefore performed to develop and characterize a better quantitative mutation assay for human cells. The uv-induction of diphtheria toxin resistant (DT/sup r/) mutations in normal and excision repair defective xeroderma pigmentosum (XP) fibroblasts has been quantitatively characterized. A concentration of diphtheria toxin to use in the selection of resistant mutants was determined whereby DT/sup r/ cells are cross-resistant to Pseudomonas aeurginosa exotoxin A, indicating mutants have altered elongation factor-2 (EF-2) which is not susceptible to ADP-ribosylation by either toxin. Results of this study indicate that XP fibroblasts have higher uv-induced mutation frequencies per unit uv-dose but similar frequencies per unit survival compared to normal cells as measured using a new genetic marker for quantitative mutagenesis. Furthermore, these results support a prediction of the mutation theory of cancer, namely, that cells from individuals with certain human syndromes that predispose the individual to cancer will have higher induced mutation frequencies than cells from non-susceptible individuals. This newly characterized genetic marker should be useful in quantitative mutagenesis studies in human cells

Potential protective immunogenicity of tetanus toxoid, diphtheria toxoid and Cross Reacting Material 197 (CRM197) when used as carrier proteins in glycoconjugates.

Science.gov (United States)

Bröker, Michael

2016-03-03

When tetanus toxoid (TT), diphtheria toxoid (DT) or Cross Reacting Material 197 (CRM197), a non-toxic diphtheria toxin mutant protein, are used as carrier proteins in glycoconjugate vaccines, these carriers induce a protein specific antibody response as measured by in vitro assays. Here, it was evaluated whether or not glycoconjugates based on TT, DT or CRM197 can induce a protective immune response as measured by potency tests according to the European Pharmacopoeia. It could be shown, that the conjugate carriers TT and DT can induce a protective immune response against a lethal challenge by toxins in animals, while glycoconjugates based on CRM197 failed to induce a protective immune response. Opportunities for new applications of glycoconjugates are discussed.

Three-dimensional measurement of femoral neck anteversion and neck shaft angle.

Science.gov (United States)

Sangeux, Morgan; Pascoe, Jessica; Graham, H Kerr; Ramanauskas, Fiona; Cain, Tim

2015-01-01

We present a three-dimensional measurement technique for femoral neck anteversion and neck shaft angles which do not require alignment of the femoral and scanner axes. Two assessors performed the measurements on 11 patients (22 femurs). Repeatability between assessors was 2.7 degrees for femoral neck anteversion and 4.8 degrees for neck shaft angle. Measurements compared with an alternative single slice method were different by 2 degrees (3 degrees) in average. The method was repeatable and appropriate for clinical practice.

Influence of snow shovel shaft configuration on lumbosacral biomechanics during a load-lifting task.

Science.gov (United States)

Lewinson, Ryan T; Rouhi, Gholamreza; Robertson, D Gordon E

2014-03-01

Lower-back injury from snow shovelling may be related to excessive joint loading. Bent-shaft snow shovels are commonly available for purchase; however, their influence on lower back-joint loading is currently not known. Therefore, the purpose of this study was to compare L5/S1 extension angular impulses between a bent-shaft and a standard straight-shaft snow shovel. Eight healthy subjects participated in this study. Each completed a simulated snow-lifting task in a biomechanics laboratory with each shovel design. A standard motion analysis procedure was used to determine L5/S1 angular impulses during each trial, as well as peak L5/S1 extension moments and peak upper body flexion angle. Paired-samples t-tests (α = 0.05) were used to compare variables between shovel designs. Correlation was used to determine the relationship between peak flexion and peak moments. Results of this study show that the bent-shaft snow shovel reduced L5/S1 extension angular impulses by 16.5% (p = 0.022), decreased peak moments by 11.8% (p = 0.044), and peak flexion by 13.0% (p = 0.002) compared to the straight-shaft shovel. Peak L5/S1 extension moment magnitude was correlated with peak upper body flexion angle (r = 0.70). Based on these results, it is concluded that the bent-shaft snow shovel can likely reduce lower-back joint loading during snow shovelling, and thus may have a role in snow shovelling injury prevention. Copyright © 2013 Elsevier Ltd and The Ergonomics Society. All rights reserved.

Removable control rod drive shaft guide

International Nuclear Information System (INIS)

Ales, M.W.; Brown, S.K.; Dixon, L.D.

1988-01-01

A removable control rod drive shaft guide is described for a control rod ''guide'' structure card, comprising: a. a substantially annular shaped main body portion having a central axial bore for receiving a control rod drive shaft and an upper exterior groove for receiving removal tooling; b. the main body portion having a reduced outer diameter at its lower section; c. a shoulder portion integral with the main body portion for supporting the main body portion on the guide structure card; d. the shoulder portion having a substantially radial bore and the reduced outer diameter lower section having a slot in alignment with the radial bore; e. a locking arm ''pivotaly'' mounted in the radial bore which protrudes into the slot and is movable between a first normal locking position for engaging the guide structure card and a second release position; f. a spring received within a second axial bore in the main body portion and biased against the locking arm for urging and locking arm into the first normal locking position; and g. a release tab at one end of the locking arm for moving the locking arm into the second release position

HOC Based Blind Identification of Hydroturbine Shaft Volterra System

Directory of Open Access Journals (Sweden)

Bing Bai

2017-01-01

Full Text Available In order to identify the quadratic Volterra system simplified from the hydroturbine shaft system, a blind identification method based on the third-order cumulants and a reversely recursive method are proposed. The input sequence of the system under consideration is an unobservable independent identically distributed (i.i.d., zero-mean and non-Gaussian stationary signal, and the observed signals are the superposition of the system output signal and Gaussian noise. To calculate the third-order moment of the output signal, a computer loop judgment method is put forward to determine the coefficient. When using optimization method to identify the time domain kernels, we combined the traditional optimization algorithm (direct search method with genetic algorithm (GA and constituted the hybrid genetic algorithm (HGA. Finally, according to the prototype observation signal and the time domain kernel parameters obtained from identification, the input signal of the system can be gained recursively. To test the proposed method, three numerical experiments and engineering application have been carried out. The results show that the method is applicable to the blind identification of the hydroturbine shaft system and has strong universality; the input signal obtained by the reversely recursive method can be approximately taken as the random excitation acted on the runner of the hydroturbine shaft system.

Influence of Shaft Torsional Stiffness on Dynamic Response of Four-Stage Main Transmission System

Directory of Open Access Journals (Sweden)

Yuan Chen

2018-01-01

Full Text Available Dynamic response analysis has potential for increasing fatigue life of the components in the transmission of a multistage main transmission system. The calculated data can demonstrate the influence of shaft torsional stiffness on dynamic characteristics of the system. Detecting key shafts of the system and analyzing their sensitivity are important for the design of four-stage helicopter gear box. Lumped mass method is applied for dynamic modeling and Fourier method is used to solve differential equation of the system. Results of the analysis indicate that key shafts can be designed carefully to improve the performance of the transmission system.

Main-coolant-pump shaft-seal guidelines. Volume 2. Operational guidelines. Final report

International Nuclear Information System (INIS)

Fair, C.E.; Greer, A.O.

1983-03-01

This report presents a set of guidelines and criteria for improving main coolant pump shaft seal operational reliability. The noted guidelines are developed from EPRI sponsored nuclear power plant seal operating experience studies. Usage procedures/practices and operational environment influence on seal life and reliability from the most recent such survey are summarized. The shaft seal and its auxiliary supporting systems are discussed both from technical and operational related viewpoints

Preventing the preventable through effective surveillance: the case of diphtheria in a rural district of Maharashtra, India.

Science.gov (United States)

Phalkey, Revati K; Bhosale, Rajesh V; Joshi, Abhijeet P; Wakchoure, Sushil S; Tambe, Muralidhar P; Awate, Pradip; Marx, Michael

2013-04-08

Epidemic diphtheria is still poorly understood and continues to challenge both developing and developed countries. In the backdrop of poor immunization coverage, non-existent adult boosters, weak case based surveillance and persistence of multiple foci, there is a heightened risk of re-emergence of the disease in epidemic forms in India. Investigating each outbreak to understand the epidemiology of the disease and its current status in the country is therefore necessary. Dhule a predominantly tribal and rural district in Northern Maharashtra has consistently recorded low vaccination coverages alongside sporaidic cases of diphtheria over the last years. This study reports the findings of an onsite survey conducted to assess a recent outbreak of diphtheria in Dhule district and the response mounted to it. Secondary data regarding outbreak detection and response were obtained from the district surveillance office. Clinical data were extracted from hospital records of eleven lab confirmed cases including one death case. Frequency distributions were calculated for each identified clinical and non- clinical variable using Microsoft™ Excel® 2010. Our findings suggest a shift in the median age of disease to adolescents (10-15 years) without gender differences. Two cases (18%) reported disease despite immunization. Clinical symptoms included cough (82%), fever (73%), and throat congestion (64%). About 64% and 36% of the 11 confirmed cases presented with a well defined pseudomembrane and a tonsillar patch respectively. Drug resistance was observed in all three culture positive cases. One death occurred despite the administration of Anti-Diphtheric Serum in a partially immunized case (CFR 9%). Genotyping and toxigenicity of strain was not possible due to specimen contamination during transport as testing facilities were unavailable in the district. The outbreak raises several concerns regarding the epidemiology of diphtheria in Dhule. The reason for shift in the median

Asymmetry in gait pattern following tibial shaft fractures

DEFF Research Database (Denmark)

Larsen, Peter; Læssøe, Uffe; Rasmussen, Sten

2017-01-01

INTRODUCTION: Despite the high number of studies evaluating the outcomes following tibial shaft fractures, the literature lacks studies including objective assessment of patients' recovery regarding gait pattern. The purpose of the present study was to evaluate whether gait patterns at 6 and 12...... months post-operatively following intramedullary nailing of a tibial shaft fracture are different compared with a healthy reference population. PATIENTS AND METHODS: The study design was a prospective cohort study. The primary outcome measurement was the gait patterns at 6 and 12 months post......-operatively measured with a 6-metre-long pressure-sensitive mat. The mat registers footprints and present gait speed, cadence as well as temporal and spatial parameters of the gait cycle. Gait patterns were compared to a healthy reference population. RESULTS: 49 patients were included with a mean age of 43.1 years (18...

Safety and reactogenicity of the combined diphtheria-tetanus-acellular pertussis-inactivated poliovirus-Haemophilus influenzae type b (DTPa-IPV/Hib) vaccine in healthy Vietnamese toddlers: An open-label, phase III study.

Science.gov (United States)

Anh, Dang Duc; Van Der Meeren, Olivier; Karkada, Naveen; Assudani, Deepak; Yu, Ta-Wen; Han, Htay Htay

2016-03-03

The introduction of combination vaccines plays a significant role in increasing vaccine acceptance and widening vaccine coverage. Primary vaccination against diphtheria, tetanus, pertussis, poliomyelitis and Haemophilus influenza type b (Hib) diseases has been implemented in Vietnam. In this study we evaluated the safety and reactogenicity of combined diphtheria-tetanus-pertussis-inactivated polio (DTPa-IPV)/Hib vaccine when administered as a booster dose in 300 healthy Vietnamese children Vietnamese children aged <2 years.

Proposal of rock mass behavior classification based on convergence measurement in shaft sinking through sedimentary soft rocks

International Nuclear Information System (INIS)

Tsusaka, Kimikazu

2010-01-01

Japan Atomic Energy Agency has been excavating deep shafts through sedimentary soft rocks in Horonobe, Hokkaido. From the viewpoint of the observational construction, site engineers need a practical guide to evaluate the field measurements conducted with shaft sinking. The author analyzed the relationship among initial deformation rate, observed deformation, the ratio of the modulus of elasticity of rock mass to the initial stress, and the magnitude of inelastic behavior of rock based on convergence measurements and investigation of rock mass properties on shaft walls. As a result, the rock mass behavior classification for shaft sinking which consists of three classes was proposed. (author)

Cloud-based shaft torque estimation for electric vehicle equipped with integrated motor-transmission system

Science.gov (United States)

Zhu, Xiaoyuan; Zhang, Hui; Yang, Bo; Zhang, Guichen

2018-01-01

In order to improve oscillation damping control performance as well as gear shift quality of electric vehicle equipped with integrated motor-transmission system, a cloud-based shaft torque estimation scheme is proposed in this paper by using measurable motor and wheel speed signals transmitted by wireless network. It can help reduce computational burden of onboard controllers and also relief network bandwidth requirement of individual vehicle. Considering possible delays during signal wireless transmission, delay-dependent full-order observer design is proposed to estimate the shaft torque in cloud server. With these random delays modeled by using homogenous Markov chain, robust H∞ performance is adopted to minimize the effect of wireless network-induced delays, signal measurement noise as well as system modeling uncertainties on shaft torque estimation error. Observer parameters are derived by solving linear matrix inequalities, and simulation results using acceleration test and tip-in, tip-out test demonstrate the effectiveness of proposed shaft torque observer design.

The treatment of nonisthmal femoral shaft nonunions with im nail exchange versus augmentation plating.

Science.gov (United States)

Park, Jin; Kim, Sul Gee; Yoon, Han Kook; Yang, Kyu Hyun

2010-02-01

The purpose of this study was to compare the results between exchange nailing (EN) and augmentation plating (AP) with a nail left in situ for nonisthmal femoral shaft nonunion after femoral nailing. : Retrospective data analysis, November 1996-March 2006. A level I trauma center. Eighteen patients with 18 nonisthmal femoral nonunions. Seven patients with 7 fractures treated for nonisthmal femoral shaft nonunions after femoral nailing with EN and 11 patients with 11 fractures treated for nonisthmal femoral shaft nonunions after nailing with AP combined with bone grafting. Union and complications. Five nonunions in the EN group failed to achieve union (72% failure rate), whereas all 11 pseudarthroses in the AP group obtained osseous union. Fisher exact test showed a higher nonunion rate of EN compared with AP for nonisthmal femoral shaft nonunion (odds ratio, 6.5; P = 0.002). AP with autogenous bone grafting may be a better option than EN for nonisthmal femoral nonunions.

Diagnosis of power generator sets by analyzing the crank shaft angular speed

International Nuclear Information System (INIS)

Desbazeille, M.

2010-07-01

This thesis deals with the diagnosis of a powerful 20-cylinder diesel engine which runs a generator set in a nuclear plant. The objective is to make a diagnosis by analyzing the crank shaft angular speed variations. Only combustion related faults are investigated. As the engine is very large, the first crank shaft natural modes are in the low frequencies. Torsional vibrations of the flexible crank shaft strongly complicate the analysis of the angular speed variations. Little attention has been paid to such large engines in the literature. First, a dynamical model with the assumption of a flexible crank shaft is established. The parameters of the model are optimized with the help of actual data. Then, an original automated diagnosis based on pattern recognition of the angular speed waveforms is proposed. Indeed, any faulty cylinder in combustion stroke will distort the angular speed waveform in a specific way which depends on its location with respect to nodes and anti-nodes of the modes. Reference patterns, representative of the engine conditions, are computed with the model constituting the main originality of this work. Promising results are obtained in operational phase. An experimental fuel leakage fault was correctly diagnosed, including detection and localization of the faulty cylinder and an indication of the severity of the fault. (author)

Postoperative malrotation of humeral shaft fracture after plating compared with intramedullary nailing.

Science.gov (United States)

Li, Ying; Wang, Cheng; Wang, Manyi; Huang, Lei; Huang, Qiang

2011-09-01

We supposed difference of rotation alignment of postoperative humeral shaft fracutres between open reduction and internal fixation (ORIF) and intramedullay nailing (IMN) could be identified and the difference might influence the involved shoulder. This study evaluated and compared the extent of malrotation and shoulder function after humeral shaft fractures treated operatively with IMN or ORIF. Fifty humeral shaft fractures were randomly allocated into 2 groups. Group I underwent antegrade IMN and group II underwent ORIF. Malrotation was measured postoperatively by computed tomography scan (CT). Fracture union and functional outcomes were recorded at 12 months. The final analysis comprised 45 patients. Group I had lower functional scores than group II (P shaft fracture. The study can provide baseline data for larger series and longer follow-up periods. Patients who underwent IMN had lower functional scores and a decreased range of motion postoperatively and also had a greater degree of malrotation than the ORIF group, which had none. The degree of malrotation correlated with a decreased range of motion and may possibly be a reason for degenerative arthritis at long-term follow-up. Copyright © 2011 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Mosby, Inc. All rights reserved.

An Experimental Evaluation of the Weathering Effects on Mine Shaft Lining Materials

Directory of Open Access Journals (Sweden)

W. Yang

2017-01-01

Full Text Available Many shaft collapses are related to the deterioration and failure of the masonry shaft lining materials. In modern mine shaft, concrete is widely used to provide support. To analyse shafts stability, the properties of the lining need to be well defined. The behaviour of masonry and concrete can be considerably affected by long-term exposure to harsh mine water. This paper presents a study which focuses on the weathering effects of mine water on lining materials (brick, mortar, and concrete. To reproduce the weathering process, samples were placed into solutions of potable water, artificial mine water, and a more aggressive mine-water solution for just less than one year. Four phases of laboratory tests were conducted throughout the time period to assess the degradation of mechanical properties of the lining materials. Particular attention is given to the degradation of material strength and stiffness. Results indicate that the harsh acidic mine water has pronounced detrimental effects on the strength and stiffness of mortar. The weathering process is shown to have the most significant effect on the stiffness of concrete and mortar. It is also shown that the use of mass loss as an index for evaluation of mechanical properties may not be appropriate.

Femoral shaft fractures in children, treaties with elastic nails of titanium - TENs

International Nuclear Information System (INIS)

Solano Urrutia, Antonio Luis; Gallon, Luis Alfonso; Echandia, Carlos

2003-01-01

Femoral shaft fractures are a common and temporarily disabling injury in children. Recent advances in orthopedic traumatology have led us at the Hospital Universitario del Valle, in Cali, Colombia, to perform a case series observational study with the use of a more aggressive approach with the new endomedullar titanium nails (TENs) in children suffering femur shaft fractures, in a closed procedure performed under image intensification. The main purpose of this study was to evaluate the outcome of 30 children treated with these nails. Pediatric femoral shaft fractures are more common in males with approximately a 2.75:1 male to female ratio. The age distribution is bimodal, with peaks in the younger than 5 years and mid-teenage groups (10-14 years) because of the increased incidence of high-energy trauma in teenage children (mean age: 9 years). Overall, TENs allowed rapid mobilization with few complications. The results were excellent or satisfactory in our cases. No child lost rotational alignment in the postoperative period. Irritation of the soft tissue near the knee by the nail tip occurred in one patient. As indications, implantation technique, and aftercare are refined, TENs may prove to be the ideal implant to stabilize most pediatric femur shaft fractures, avoiding the prolonged immobilization and complications of traction and spica cast

IMMUNOGENICITY AND SAFETY OF QUINVAXEM® (DIPHTHERIA, TETANUS, WHOLE-CELL PERTUSSIS, HEPATITIS B AND HAEMOPHILUS INFLUENZAE TYPE B VACCINE) GIVEN TO VIETNAMESE INFANTS AT 2 TO 4 MONTHS OF AGE.

Science.gov (United States)

Huu, Tran Ngoc; Phuong, Nguyen Thi Minh; Toan, Nguyen Trong; Thang, Ho Vinh

2015-07-01

Vietnam plans to replace the routine childhood diphtheria, pertussis and tetanus combination (DPT) vaccine with a pentavalent vaccine. The present study was performed to assess the immunogenicity and safety of the combined diphtheria, tetanus, whole-cell pertussis, hepatitis B (HepB), and Haemophilus influenzae type b (Hib) (DTwP-HepB-Hib) Quinvaxem® vaccine in children. A total of 131 infants received the Quinvaxem® vaccine at 2, 3 and 4 months. Antibody levels were measured at baseline, at one month after the third injection and one year after the first injection. Seroprotection rates were high for each vaccine antigen at one month after the third dose: 93.1% for diphtheria, 98.5% for tetanus, 99.2% for pertussis (seroconversion rate), 93.1% for HepB, and 100% for Hib (anti-PRP ≥ 0.15 µg/ml). The rate of children with protective antibodies persisting at one year after the first dose was 88.4% for diphtheria, 49.6% for pertussis, 82.2% for tetanus, 76.7% for HepB and 97.7% for Hib (anti-PRP ≥ 0.15 µg/ml). The Quinvaxem® vaccine was well tolerated and has a low rate of adverse events. Quinvaxem® given at 2, 3 and 4 months of age was immunogenic and safe for primary immunization among infants in Vietnam.

Transporting large monolithic shafts through the interior of the city of Rome

Directory of Open Access Journals (Sweden)

Patrizio Pensabene

2017-01-01

Full Text Available One of the questions we have to answer when studying the process of building Rome’s huge temples is how their large monolithic shafts were moved through the city. The shafts used in some of the temples were enormous. For example, the temple of Antoninus and Faustina in the Roman Forum and the Forum of Peace were built, respectively, with shafts of cipollino and granito rosa from Aswan. They would have had to have been taken through the city centre where manoeuvring such large loads through the narrow streets would not have been an easy task. We will attempt to hypothesise not only the possible routes along which these enormous blocks were transported, but also the mechanical operations that would have been needed to overcome the frequent obstacles that would have been encountered.

Pump shaft failures - a compendium of case studies

CSIR Research Space (South Africa)

Bernt, F

2001-04-01

Full Text Available During operation, pump shafts usually suffer from degradation as a result of corrosion and/or mechanical degradation, usually in the form of fatigue failures. In many cases corrosion precedes fatigue failure and can actually accelerate the rate...

Effect of the shaft on the aerodynamic performance of urban vertical axis wind turbines

NARCIS (Netherlands)

Rezaeiha, A.; Kalkman, I.; Montazeri, H.; Blocken, B.J.E.

2017-01-01

The central shaft is an inseparable part of a vertical axis wind turbine (VAWT). For small turbines such as those typically used in urban environments, the shaft could operate in the subcritical regime, resulting in large drag and considerable aerodynamic power loss. The current study aims to (i)

Response to Comment on "Whole-Genome Shotgun Sequencing of Mitochondria from Ancient Hair Shafts"

DEFF Research Database (Denmark)

Gilbert, Marcus Thomas Pius; Miller, Webb; Schuster, Stephan C.

2008-01-01

Debruyne et al. challenge the findings of our study and imply that we argue that hair shafts are an overall superior source of ancient DNA than bone. However, the authors are misreading and misinterpreting the conclusions of our study; we claim nothing further than that hair shaft represents...

Evaluation and validation of a single-dilution potency assay based upon serology of vaccines containing diphtheria toxoid: statistical analysis

NARCIS (Netherlands)

Marsman FR; Akkermans AM; Hendriksen CFM; de Jong WH

1993-01-01

This document presents the results of a validation study to the use of a single dilution assay in potency testing of the diphtheria component of DPT-polio vaccines. Based on historical data of multi-dilution assays on 27 consecutive batches a simulation study was performed to test the actual

Main-coolant-pump shaft-seal guidelines. Volume 3. Specification guidelines. Final report

International Nuclear Information System (INIS)

Fair, C.E.; Greer, A.O.

1983-03-01

This report presents a set of guidelines and criteria to aid in the generation of procurement specifications for Main Coolant Pump Shaft Seals. The noted guidelines are developed from EPRI sponsored nuclear power plant seal operating experience studies, a review of pump and shaft seal literature and discussions with pump and seal designers. This report is preliminary in nature and could be expanded and finalized subsequent to completion of further design, test and evaluation efforts

A Unit-Cell Model for Predicting the Elastic Constants of 3D Four Directional Cylindrical Braided Composite Shafts

Science.gov (United States)

Hao, Wenfeng; Liu, Ye; Huang, Xinrong; Liu, Yinghua; Zhu, Jianguo

2018-06-01

In this work, the elastic constants of 3D four directional cylindrical braided composite shafts were predicted using analytical and numerical methods. First, the motion rule of yarn carrier of 3D four directional cylindrical braided composite shafts was analyzed, and the horizontal projection of yarn motion trajectory was obtained. Then, the geometry models of unit-cells with different braiding angles and fiber volume contents were built up, and the meso-scale models of 3D cylindrical braided composite shafts were obtained. Finally, the effects of braiding angles and fiber volume contents on the elastic constants of 3D braided composite shafts were analyzed theoretically and numerically. These results play a crucial role in investigating the mechanical properties of 3D 4-directional braided composites shafts.

Natural Frequncies of Coupled Blade-Bending and Shaft-Torsional Vibrations

Directory of Open Access Journals (Sweden)

B.O. Al-Bedoor

2007-01-01

Full Text Available In this study, the coupled shaft-torsional and blade-bending natural frequencies are investigated using a reduced order mathematical model. The system-coupled model is developed using the Lagrangian approach in conjunction with the assumed modes method to discretize the blade bending deflection. The model accounts for the blade stagger (setting angle, the system rotating speed and its induced stiffening effect. The coupled equations of motion are linearized based on the small deformation theory for the blade bending and shaft torsional deformation to enable calculation of the system natural frequencies for various combinations of system parameters. The obtained coupled eignvalue system is ready for use as a reference for comparison for larger size finite element simulations and for the use as a fast check on natural frequencies for the coupled blade bending and shaft torsional vibrations in the design and diagnostics processes. Some results on the predicted natural frequencies are graphically presented and discussed pertinent to the coupling controlling factors and their effects. In addition, the predicted coupled natural frequencies are validated using the Finite Element Commercial Package (Pro-Mechanica where good agreements are found.

Operating reliability of the shaft seal system of ANDRITZ RCP

International Nuclear Information System (INIS)

Grancy, Werner; Zehentner, Martin

2002-01-01

The next generation of nuclear power stations will have to fulfil new expectations in terms of safety, operating behaviour and costs. This applies also and especially to reactor coolant pumps for the primary circuit of pressurized water reactor type nuclear power plants (RCP). For 4 decades, ANDRITZ AG has developed and built RCPs and has attached great importance to the design of the complete pump rotor and of its essential surrounding elements, such as e. g. the shaft seal. Many questions concerning design and configuration of the shaft seal system cannot be answered purely theoretically, or they can only be answered partly. Therefore, comprehensive development work and testing was necessary to increase the operating reliability of the seal. Apart from all relevant questions connected with design and functioning of the pump there is one question of top priority: the operating reliability of the shaft seal system. Therefore it is intended to describe the current status of design and development of ANDRITZ RCP for future Korean NPPs, to present the most important design features and to give an introduction concerning experiences for a 3-stage-hydrodynamic seal as well as for a 2-stage-hydrodynamic seal

Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen/deuterium exchange measurements

Czech Academy of Sciences Publication Activity Database

Man, Petr; Montagner, C.; Vitrac, H.; Kavan, Daniel; Pichard, S.; Gillet, D.; Forest, E.; Forge, V.

2010-01-01

Roč. 277, č. 3 (2010), s. 653-662 ISSN 1742-464X Institutional research plan: CEZ:AV0Z50200510 Keywords : diphtheria toxin * hydrogen/deuterium exchanges * mass spectrometry Subject RIV: CE - Biochemistry Impact factor: 3.129, year: 2010

Ipsilateral femoral shaft and vertical patella fracture: a case report

Science.gov (United States)

Ozkan, Korhan; Eceviz, Engin; Sahin, Adem; Ugutmen, Ender

2009-01-01

Introduction A femoral shaft fracture with an ipsilateral patella fracture has been, to our knowledge, given only cursory attention in English-speaking literature. Case presentation A 15 year old male patient had hitten by a car to his motorcycle came to emergency room and he had been operated for his femoral shaft freacture and vertical patellar fracture which was iniatally missed. Conclusion To us it is vital to obtain CT scan of the patient’s knee if there is an ipsilateral femoral fracture with an ipsilateral knee effusion and a punction which reveals hematoma even in the absence of a fracture line seen in AP and lateral projections. PMID:19829933

Mine shaft fire and smoke protection systems - an update on hardware development and in-mine testing

International Nuclear Information System (INIS)

Johnson, G.A.

1982-01-01

In 1976, The Bureau of Mines developed a prototype system to sense and extinguish fires in shafts and shaft stations in underground metal and nonmetal mines. Subsequent work modified this technology to include fueling areas, spontaneous combustion zones and coal mines. This paper updates IC-8783 ''In-mine Fire Tests of Mine Shaft Fire and Smoke Protection Systems'', which was published in 1978 and summarized the design and in-mine, actual fire testing of the first prototype mine shaft fire and smoke protection system. This paper also updates related work from IC-8775 ''Spontaneous Oxidation and Combustion of Sulfide Ores in Underground Mines, (also published in 1978) and IC-8808 ''In-mine Evaluation of Underground Fire and Smoke Detectors'', (published in early 1979)

Application of a hydrophilic Fe-Co magnetic fluid to the oil seal of a rotary shaft

International Nuclear Information System (INIS)

Lee, J. H.; Ryu, B. O.; Song, W. S.; Hong, G. P.; Zoo, Y. S.

2003-01-01

Existing oil seals of rotary shafts are made of rubber or ceramic goods (rubber retainer or mechanical seal). Thus if they are used for a long time, lubricant's leakage is induced from the gap between the shaft and bearings because of stiffening and abrading on the quality of seals due to the friction between rotating shaft and oil seal. Therefore the oil seals is restricted to durability limits and caused to require a quick change of the seal parts and to require significant man - powers for the complicated fabrication of seals. This study is established from the idea for working out these problems. This seal is composed of magnetic fluid to stop up oil in seals. As magnetic fluid between shaft and oil seal stops up oil in seals during rotating shaft, there is a friction but isn't an abrasion between shaft and oil seal so that there is no problem of the durability limits. In this study, with Fe- Co magnetic fluid is produced by hydrophilic ethylene glycol medium, Fe- Co(30 % : Co) powder, ring structure's Nd- permanent magnet of magnetic field strength 3300 Gauss and pole-piece(thickness : 1 mm, mild steel plate). With this arrangement the performance is such that the maximum resisting pressure of the oil seal apparatus was measured to be 25 kg/ cm 2 at the shaft speed 1800 rpm. It is believed that this magnetic fluid of Fe-Co powder used at the oil seal apparatus is the highest value among magnetic fluids in use until now. In an innovation this can give the advantages of lower noise, longer durability, and airtight of sealing as the contact of shaft (solid) to be friction and magnetic fluid(liquid) to seal. For that reason, this magnetic fluid of Fe-Co powder not only has enough specificity about the oil seal of rotary shaft but also shows enough quality as resisting pressure seal apparatus. Applications of this seal include all kinds of pump like high damping seal. This seal apparatus is economical and has an excellent sealing efficiency which can not be

Enhanced sealing project (ESP): evolution of a full-sized concrete and bentonite shaft seal

International Nuclear Information System (INIS)

Dixon, D.A.; Priyanto, D.G.; Martino, J.B.; De Combarieu, M.; Johansson, R.; Korkeakoski, P.; Villagran, J.

2012-01-01

Document available in extended abstract form only. A full-scale shaft seal was designed and installed in the 5-m-diameter access shaft at Atomic Energy of Canada's (AECL's) Underground Research Laboratory (URL) at the point where he shaft intersects an ancient water-bearing, low-angle thrust fault in granitic rock. The seal, part of the permanent closure of the URL, consists of a 6-m-thick bentonite-based component sandwiched between 3-m-thick upper and lower, 3-m-thick concrete components. The bentonite-based component spans the fracture zone and extends approximately 1 m beyond the maximum identified extent of the fracture. This design was adopted in order to limit water from the deeper, saline regions mixing with the fresher, near-surface groundwater regime. The concrete components provide the mechanical confinement and an in situ compacted 40/60 mixture of bentonite clay and quartz sand provides the sealing component. Construction of the shaft seal was done as part of Canada's Nuclear Legacies Liability Program. However, monitoring the seal evolution was not part of the decommissioning program's mandate. In addition to accomplishing the permanent closure of the URL, this seal's construction provides a unique opportunity to instrument and monitor the evolution of a full-scale shaft seal as well as the recovery of the regional groundwater regime as the facility passively floods above the seal. A jointly funded monitoring project was developed by NWMO (Canada), SKB (Sweden), Posiva (Finland) and ANDRA (France) and since mid 2009 the thermal, hydraulic and mechanical evolution of the seal has been constantly monitored. The evolution of the type of seal being monitored in the ESP is of relevance to repository closure planning, gaining confidence in the functionality of shaft seals. Although constructed in a crystalline rock medium, the results of the ESP are expected to be relevant to the performance of seals in a variety of host rock types. The shaft seal has been

The effect of vitamin A supplementation and diphtheria-tetanus-pertussis vaccination on parasitaemia in an experimental murine malaria model

DEFF Research Database (Denmark)

Jørgensen, Mathias Jul; Hein-Kristensen, Line; Hempel, Casper

2011-01-01

infectious diseases when given with the diphtheria-tetanus-pertussis (DTP) vaccine. The immunological effects of combining the 2 treatments are unknown. Methods: We studied the effect of treating C57BL/6 mice with VAS and DTP, 1 week prior to infection with Plasmodium berghei ANKA. The progression of disease...

Favourable environmental factors for shaft furnace installation for gas carbonitriding and hardening

Energy Technology Data Exchange (ETDEWEB)

Fuchs, H. (Solo-Industrieofenbau, Biehl-Bienne (Switzerland))

In this lecture we do not primarily speak about the shaft furnace installation as one could think when reading the title - but about one quenching system - mounted over the shaft furnace installation. This quenching system permits a hardening in several mediums, without any formation of flames, smoke and vapour, and causes the surfaces of the pieces to be free of tinder. Moreover, the security for the work staff in the heat treatment shops are highly improved.

Study of Ender’s Nailing in Shaft Femur Fractures of Older Children

Directory of Open Access Journals (Sweden)

Ladani HG