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Sample records for dermatophagoides pteronyssinus acari

  1. Dermatophagoides pteronyssinus (Trouessart, 1897) in mattress and floor dust in a temperate climate (Acari : Pyroglyphidae)

    NARCIS (Netherlands)

    Bronswijk, van J.E.M.H.

    1973-01-01

    The arthropod fauna of mattress dust, bedroom dust and living-room dust was sampled during a 1-year period in a center for asthmatic children (near Nijmegen, The Netherlands) with the aid of a vacuum cleaner, Berlese funnels and a flotation method. Dermatophagoides pteronyssinus was most abundant;

  2. Acaricidal activities of some essential oils and their monoterpenoidal constituents against house dust mite, Dermatophagoides pteronyssinus (Acari: Pyroglyphidae).

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    Saad, El-Zemity; Hussien, Rezk; Saher, Farok; Ahmed, Zaitoon

    2006-12-01

    The acaricidal activities of fourteen essential oils and fourteen of their major monoterpenoids were tested against house dust mites Dermatophagoides pteronyssinus. Five concentrations were used over two different time intervals 24 and 48 h under laboratory conditions. In general, it was noticed that the acaricidal effect based on LC(50) of either essential oils or monoterpenoids against the mite was time dependant. The LC(50) values were decreased by increasing of exposure time. Clove, matrecary, chenopodium, rosemary, eucalyptus and caraway oils were shown to have high activity. As for the monoterpenoids, cinnamaldehyde and chlorothymol were found to be the most effective followed by citronellol. This study suggests the use of the essential oils and their major constituents as ecofriendly biodegradable agents for the control of house dust mite, D. pteronyssinus.

  3. Acaricidal effects of herb essential oils against Dermatophagoides farinae and D. pteronyssinus (Acari: Pyroglyphidae) and qualitative analysis of a herb Mentha pulegium (pennyroyal)

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    Rim, In-Sook

    2006-01-01

    This experiment was undertaken to screen the acaricidal effects of herb essential oils (pennyroyal, ylang ylang, citronella, lemon grass, tea tree, and rosemary) at different doses (0.1, 0.05, 0.025, 0.0125, and 0.00625 µl/cm2) and exposure times (5, 10, 20, 20, 30 and 60 min) on house dust mites Dermatophgoides farinae and D. pteronyssinus. The most effective acaricidal components of pennyroyal (Mentha pulegium) were analyzed using a gas chromatography-mass spectrometer (GC-MS). Of these essential oils, the most effective was pennyroyal, which is composed essentially of pulegone (> 99%), at a dose of 0.025 µl/cm2 which at an exposure time of 5 min killed more than 98% of house dust mites. In the pennyroyal fumigation test, the closed method was more effective than the open method and maximum acaricidal effect was 100% at 0.025 µl/cm2, 60 min. The results show that herb essential oils, in particular, pennyroyal was proved to have potent acaricidal activity. PMID:16809961

  4. Pheromonal Communication in the European House Dust Mite, Dermatophagoides pteronyssinus

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    Johannes L.M. Steidle

    2014-08-01

    Full Text Available Despite the sanitary importance of the European house dust mite Dermatophagoides pteronyssinus (Trouessart, 1897, the pheromonal communication in this species has not been sufficiently studied. Headspace analysis using solid phase micro extraction (SPME revealed that nerol, neryl formate, pentadecane, (6Z,9Z-6,9-heptadecadiene, and (Z-8-heptadecene are released by both sexes whereas neryl propionate was released by males only. Tritonymphs did not produce any detectable volatiles. In olfactometer experiments, pentadecane and neryl propionate were attractive to both sexes as well as to tritonymphs. (Z-8-heptadecene was only attractive to male mites. Therefore it is discussed that pentadecane and neryl propionate are aggregation pheromones and (Z-8-heptadecene is a sexual pheromone of the European house dust mite D. pteronyssinus. To study the potential use of pheromones in dust mite control, long-range olfactometer experiments were conducted showing that mites can be attracted to neryl propionate over distances of at least 50 cm. This indicates that mite pheromones might be useable to monitor the presence or absence of mites in the context of control strategies.

  5. The Differences in Serum Quantitative Specific IgE Levels Induced by Dermatophagoides pteronyssinus, Dermatophagoides farinae and Blomia tropicalis Sensitization in Intermittent and Persistent Allergic Asthma

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    Agus Joko Susanto

    2018-01-01

    Full Text Available Background: house dust mites (HDM are an important inhalant allergen in allergic asthma. However, molecular diagnostic study of specific IgE to HDM allergens has not been done in Indonesia. In addition, the association of quantitative specific IgE measurement with asthma severity has not been investigatedd. This study aimed to investigate the difference of serum quantitative specific IgE levels induced by Dermatophagoides (D. pteronyssinus, D. farinae and Blomia tropicalis sensitization in intermittent and persistent allergic asthma. Methods: this was a cross-sectional study on adult allergic asthma patients who were invited for serum specific IgE testing. This study was a part of a larger study within the Division of Allergy and Immunology, Cipto Mangunkusumo Hospital. Asthma severity was defined based on Global Initiative on Asthma (GINA 2015 criteria and were grouped as intermittent or persistent. Quantitative specific IgE testing was done on blood serum using a multiple allergosorbent test (Polycheck Allergy, Biocheck GmbH, Munster, Germany. The HDM allergens tested were D. pteronyssinus, D. farinae, and Blomia tropicalis. Difference between two groups were analyze using Mann-Whitney test. Results: a total of 87 subjects were enrolled in this study; 69 (79.3% were women. Mean patients’ age was 40, 2 years. Sixty-three (72.4% subjects had asthma and allergic rhinitis. Fifty-eight (66.7% subjects were classified as persistent asthma. The prevalence of sensitization was 62.1% for D. farinae, 51.7% for D. pteronyssinus, and 48.3% for Blomia tropicalis. The median of specific IgE levels were significantly higher in persistent asthma compares to intermittent asthma induced by D. farinae (median 1.30 vs. 0.0 kU/L; p=0.024 and B. tropicalis (median 0.57 vs. 0.0 kU/L; p=0.015 sensitization. Level of Specific IgE  D. pteronyssinus was also to be higher in persistent asthma than the level measured in intermittent asthma (0.67 vs. 0.00 kU/L; p=0

  6. IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract

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    Maciel M.

    2004-01-01

    Full Text Available Induced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. However, the influence of oral tolerance on specific and natural polyreactive IgA antibodies, the major defense mechanism of the mucosa, is unknown. We have shown that oral administration of an extract of the dust mite Dermatophagoides pteronyssinus (Dp to primed mice caused down-regulation of IgE responses and an increase in tumor growth factor-ß secretion. In the present study, we observed that primed inbred female A/Sn mice (8 to 10 weeks old fed by gavage a total weight of 1.0-mg Dp extract on the 6th, 7th and 8th days post-immunization presented normal secretion of IL-4 and IL-10 in gut-associated lymphoid tissue and a decreased production of interferon gamma induced by Dp in the draining lymph nodes (13,340 ± 3,519 vs 29,280 ± 2,971 pg/ml. Mice fed the Dp extract also showed higher levels of serum anti-Dp IgA antibodies and an increase of IgA-secreting cells in mesenteric lymph nodes (N = 10, reflecting an increase in total fecal IgA antibodies (N = 10. The levels of secretory anti-Dp IgA antibodies increased after re-immunization regardless of Dp extract feeding. Oral tolerance did not interfere with serum or secretory IgA antibody reactivity related to self and non-self antigens. These results suggest that induction of oral tolerance to a Dp extract in sensitized mice triggered different regulatory mechanisms which inhibited the IgE response and stimulated systemic and secretory IgA responses, preserving the natural polyreactive IgA antibody production.

  7. Comparative analysis of biological activities of Der p I-derived peptides on Fc epsilon receptor-bearing cells from Dermatophagoides pteronyssinus-sensitive patients.

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    Jeannin, P; Pestel, J; Bossus, M; Lassalle, P; Tartar, A; Tonnel, A B

    1993-01-01

    The ability of four uncoupled synthetic peptides (p52-71, p117-133, p176-187, p188-199) derived from Der p I, a major allergen from the house dust mite Dermatophagoides pteronyssinus (Dpt) to stimulate Fc epsilon R+ cells from Dpt-sensitive patients was comparatively analysed. Each free peptide may specifically stimulate basophils (Fc epsilon RI+ cells) and platelets (Fc epsilon RII+ cells) from patients with significant levels of anti-Der p I IgE antibodies; p52-71 and p117-133 appear the best cell stimulation inducers. Both concentration-dependent biological activities of Der p I-peptide on Fc epsilon R+ cells are enhanced by coupling peptide to a carrier (as human serum albumin). Interestingly each Der p I-sensitive patient tested presents an individual pattern of response to peptide. Thus, from our results it appears that different Der p I sequences could be involved in the immune response to Der p I. PMID:7682161

  8. Comparative analysis of biological activities of Der p I-derived peptides on Fc epsilon receptor-bearing cells from Dermatophagoides pteronyssinus-sensitive patients.

    Science.gov (United States)

    Jeannin, P; Pestel, J; Bossus, M; Lassalle, P; Tartar, A; Tonnel, A B

    1993-04-01

    The ability of four uncoupled synthetic peptides (p52-71, p117-133, p176-187, p188-199) derived from Der p I, a major allergen from the house dust mite Dermatophagoides pteronyssinus (Dpt) to stimulate Fc epsilon R+ cells from Dpt-sensitive patients was comparatively analysed. Each free peptide may specifically stimulate basophils (Fc epsilon RI+ cells) and platelets (Fc epsilon RII+ cells) from patients with significant levels of anti-Der p I IgE antibodies; p52-71 and p117-133 appear the best cell stimulation inducers. Both concentration-dependent biological activities of Der p I-peptide on Fc epsilon R+ cells are enhanced by coupling peptide to a carrier (as human serum albumin). Interestingly each Der p I-sensitive patient tested presents an individual pattern of response to peptide. Thus, from our results it appears that different Der p I sequences could be involved in the immune response to Der p I.

  9. 8 Allergenic Composition of Polymerized Allergen Extracts of Betula verrucosa, Dermatophagoides Pteronyssinus and Phleum Pratense

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    Fernandez-Caldas, Enrique; Cases, Barbara; Tudela, Jose Ignacio; Fernandez, Eva Abel; Casanovas, Miguel; Subiza, Jose Luis

    2012-01-01

    Background Allergoids have been successfully used in the treatment of respiratory allergic diseases. They are modified allergen extracts that allow the administration of high allergen doses, due to their reduced IgE binding capacity.They maintain allergen-specific T-cell recognition. Since they are native allergen extracts that have been polymerized with glutaraldehyde, identification of the allergenic molecules requires more complicated methods. The aim of the study was to determine the qualitative composition of different polymerized extracts and investigate the presence of defined allergenic molecules using Mass spectrometry. Methods Proteomic analysis was carried out at the Proteomics Facility of the Hospital Nacional de Parapléjicos (Toledo, Spain). After reduction and alkylation, proteins were digested with trypsin and the resulting peptides were cleaned using C18 SpinTips Sample Prep Kit; peptides were separated on an Ultimate nano-LC system using a Monolithic C18 column in combination with a precolumn for salt removal. Fractionation of the peptides was performed with a Probot microfraction collector and MS and MS/MS analysis of offline spotted peptide samples were performed using the Applied Biosystems 4800 plus MALDI TOF/TOF Analyzer mass spectrometer. ProteinPilot Software V 2.0.1 and the Paragon algorithm were used for the identification of the proteins. Each MS/MS spectrum was searched against the SwissProt 2010_10 database, Uniprot-Viridiplantae database and Uniprot_Betula database. Results Analysis of the peptides revealed the presence of native allergens in the polymerized extracts: Der p 1, Der p 2, Der p 3, Der p 8 and Der p 11 in D. pteronyssinus; Bet v 2, Bet v 6, Bet v 7 and several Bet v 1 isoforms in B. verrucosa and Phl p 1, Phl p 3, Phl p 5, Phl p 11 and Phl p 12 in P. pratense allergoids. In all cases, potential allergenic proteins were also identified, including ubiquitin, actin, Eenolase, fructose-bisphosphate aldolase, luminal

  10. [Acaricidal activity of clove bud oil against Dermatophagoides farinae (Acari: Pyroglyphidae)].

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    Li, Jing; Wu, Hai-Qiang; Liu, Zhi-Gang

    2009-12-01

    Volatile oil from the clove bud was extracted by petroleum ether using Soxhlet Extractor. The acaricidal activity was examined using direct contact and vapour phase toxicity bioassays. In a filter paper contact toxicity bio-assay, at 2.5 h after treatment, clove bud oil at a dose of 12.20 microg/cm2 killed all dust mites. As judged by 24-h LD50 values, potent fumigant action was observed with clove bud oil (12.20 microg/cm2), showing an adequate acaricidal activity against indoor Dermatophagoides farinae.

  11. On the abundance of Dermatophagoides pteronyssinus (Trouessart, 1897) (Pyroglyphidae: Acarina) in house dust

    NARCIS (Netherlands)

    Bronswijk, van J.E.M.H.; Schoonen, J.M.C.P.; Berlie, M.A.F.; Lukoschus, F.S.

    1971-01-01

    The dominance of the Pyroglyphidae in house dust has been influenced by a number of factors. The humidity in houses dropped recently to such an extent that only Pyroglyphidae can survive. When temperature and relative humidity are favorable for Acarus siro and Glycyphagus destructor as well as

  12. 8 Allergenic Composition of Polymerized Allergen Extracts of Betula verrucosa, Dermatophagoides Pteronyssinus and Phleum Pratense

    OpenAIRE

    Fernandez-Caldas, Enrique; Cases, Barbara; Tudela, Jose Ignacio; Fernandez, Eva Abel; Casanovas, Miguel; Subiza, Jose Luis

    2012-01-01

    Background Allergoids have been successfully used in the treatment of respiratory allergic diseases. They are modified allergen extracts that allow the administration of high allergen doses, due to their reduced IgE binding capacity.They maintain allergen-specific T-cell recognition. Since they are native allergen extracts that have been polymerized with glutaraldehyde, identification of the allergenic molecules requires more complicated methods. The aim of the study was to determine the qual...

  13. Solid-phase radioimmunoassay for the detection fo IgG antibodies to gramineae pollen (Dactylis glomerata) and to acarida (dermatophagoides Pteronyssinus)

    International Nuclear Information System (INIS)

    Guilloux, L.; Ville, G.

    1985-01-01

    A solid-phase radioimmunoassay using 125 I-protein A onto polystyrene microplates, was applied successfully for the detection of IgG antibodies to acarida and to grass pollen in allergic patients and in patients undergoing desensitization therapy [fr

  14. Solid-phase radioimmunoassay for the detection fo IgG antibodies to gramineae pollen (Dactylis glomerata) and to acarida (dermatophagoides Pteronyssinus)

    Energy Technology Data Exchange (ETDEWEB)

    Guilloux, L.; Ville, G.

    1985-01-01

    A solid-phase radioimmunoassay using SVI-protein A onto polystyrene microplates, was applied successfully for the detection of IgG antibodies to acarida and to grass pollen in allergic patients and in patients undergoing desensitization therapy.

  15. The Dermatophagoides farinae group 22 allergen: cloning and expression in Escherichia coli.

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    Cui, Yu-bao; Cai, Hong-xing; Zhou, Ying; Wang, Nan; Yu, Li-li; Yang, Li; Zhang, Cheng-bo

    2015-09-01

    Dermatophagoides farinae (Hughes) (Acari: Pyroglyphidae) and other domestic mites produce allergens that affect people worldwide. Here, the complementary DNA (cDNA) coding for group 22 allergen of D. farinae (Der f 22) from China was cloned, sequenced, and expressed successfully. The cDNA encoding Der f 22 was synthesized by reverse transcription polymerase chain reaction (RT-PCR), then ligated to the pCold-TF for expression in Escherichia coli BL21 cells. The purified recombinant fusion protein was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Western-blotting, and tandem matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF/TOF). The full-length cDNA comprised 468 nucleotides and was 99.57% (466/468) identical with the reference sequence (GenBank: DQ643992). After the plasmid pCold-TF-Der f 22 was transformed into E. coli BL21 and expressed with the induction of IPTG, SDS-PAGE showed a specific band for the recombinant fusion protein. The recombinant fusion protein, which was purified by chromatography, bound with a His-tagged antibody by Western blotting. MALDI-TOF/TOF mass spectrometry revealed that the structure of the recombinant protein was identical to the predicted Der f 22 structure. The hydrophilic protein contains a signal peptide of 20 amino acids, and the mature Der f 22 consists of 135 amino acid residues with a molecular weight of 14.7 kDa and theoretical isoelectric points (pI) of 6.38. Its secondary structure comprises an alpha helix (38.5%), beta-sheet (45.9%), random coils (11.85%), and beta-turns (11.1%). This work represents the first reported full-length sequence and successful cloning of Der f 22 from D. farinae in China; bioinformatics analysis can be used to further study the allergenicity and clinical utility of the recombinant Der f 22. © 2015 ARS-AAOA, LLC.

  16. The Immunomodulatory Effect of You-Gui-Wan on Dermatogoides-pteronyssinus-Induced Asthma

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    Li-Jen Lin

    2012-01-01

    Full Text Available The traditional Chinese medicine You-Gui-Wan (YGW contains ten species of medicinal plants and has been used to improve health in remissive states of asthma for hundreds of years in Asia. However, little is known about the immunomodulatory mechanisms in vivo. Therefore, this study investigated the pathologic and immunologic responses to YGW in mice that had been repeatedly exposed to Dermatogoides-pteronyssinus (Der p. YGW reduced Der-p-induced airway hyperresponsiveness and total IgE in serum. It also inhibited eosinophil infiltration by downregulating the protein expression of IL-5 in serum and changed the Th2-bios in BALF by upregulating IL-12. Results of the collagen assay and histopathologic examination showed that YGW reduced airway remodeling in the lung. In addition, after YGW treatment there was a relative decrease in mRNA expression of TGF-β1, IL-13, eotaxin, RANTES, and MCP-1 in lung in the YGW group. The results of EMSA and immunohistochemistry revealed that YGW inhibited NF-κB expression in epithelial lung cells. YGW exerts its regulative effects in chronic allergic asthmatic mice via its anti-inflammatory activity and by inhibiting the progression of airway remodeling.

  17. Der-p2 (Dermatophagoides pteronyssinus) allergen-like protein from the hard tick Ixodes ricinus - a novel member of ML (MD-2-related lipid-recognition) domain protein family

    Czech Academy of Sciences Publication Activity Database

    Horáčková, Jana; Rudenko, Natalia; Golovchenko, Maryna; Grubhoffer, Libor

    2010-01-01

    Roč. 137, č. 7 (2010), s. 1139-1149 ISSN 0031-1820 R&D Projects: GA ČR(CZ) GA524/06/1479 Institutional research plan: CEZ:AV0Z60220518 Keywords : Der-p2 allergen-like protein * Ixodes ricinus * tick, * ML protein family * IgE-binding activity Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.522, year: 2010

  18. Antigenic relationship between the house dust mite Dermatophagoides farinae and the predacious mite Phytoseiulus persimilis.

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    Homma, R; Ando, T; Miyahara, A; Kimura, H; Ito, G; Uesato, N; Ino, Y; Iwaki, M

    1994-12-01

    We have examined the antigenic relationship between the house dust mite Dermatophagoides farinae and the predacious mite Phytoseiulus persimilis. Immunoblotting analysis demonstrated that there was a very weak antigenic cross-reactivity between these different suborder of mites but that this cross-reactivity was not attributed to D. farinaes major allergen's, Der fI and Der fII. These results suggest that P. persimilis might scarcely provoke allergic symptoms in patients sensitized to house dust mites.

  19. [Onset time and efficacy of sublingual immunotherapy with Dermatophagoides farinae drops in children with allergic rhinitis].

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    Chen, Zhiling; Qian, Yasheng; Liu, Suqin; Huang, Liqin; Xu, Shiying; Yin, Wenhua; Chen, Yanchun; Wu, Huawei; Wang, Gan

    2015-08-01

    To investigate the onset time and efficacy of sublingual immunotherapy (SLIT) with Dermatophagoides farinae drops in children with house dust mites (HDM)-induced allergic rhinitis (AR). One hundred and forty three children with perennial moderate to severe HDM-induced AR were treated by SLIT with standardized Dermatophagoides farinae extract. One hundred children who finally completed two years treatment were divided into two groups according to the age: younger children group (aged 4-8 years, n = 52) and older children group (aged 9-14 years, n = 48). Respectively, Each children was assessed before and after 1st, 2nd, 3rd, 6th, 12th, 24th months of the treatment. Total nasal symptom score (TNSS), total medication score (TMS) and visual analogue scale (VAS) were evaluated at each visit. All clinical data were analyzed retrospectively with the SPSS 19.0 software. TNSS, TMS and VAS of two groups decreased significantly after three months of the treatment compared with before (younger children group: Z value was -3.843, -3.534, -3.940, older children group: Z value was -3.938, -3.405, -3.953, all P < 0.05). TNSS and VAS of younger children group decreased significantly after two months of the treatment compared with before (6.4 ± 1.6, 5.3 ± 1.4 vs 8.6 ± 1.2, 7.9 ± 1.6, Z value was -3.843, -3.940, both P < 0.05). Five children (5%) experienced local adverse events and 2 children (2%) experienced mild systemic adverse events. No severe adverse events happened during the treatment. SLIT with Dermatophagoides farinae drops is an efficient and safe treatment for children with HDM-induced AR. Its onset of action can be observed as early as 3 months after treatment.

  20. New species of Uropodina from Madagascar (Acari: Mesostigmata)

    Czech Academy of Sciences Publication Activity Database

    Kontschán, J.; Starý, Josef

    2014-01-01

    Roč. 3895, č. 4 (2014), s. 547-569 ISSN 1175-5326 Institutional support: RVO:60077344 Keywords : Acari * Mesostigmata * Uropodina * new genus * new species * Madagascar Subject RIV: EG - Zoology Impact factor: 0.906, year: 2014

  1. In Silico Prediction of T and B Cell Epitopes of Der f 25 in Dermatophagoides farinae

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    Xiaohong Li

    2014-01-01

    Full Text Available The house dust mites are major sources of indoor allergens for humans, which induce asthma, rhinitis, dermatitis, and other allergic diseases. Der f 25 is a triosephosphate isomerase, representing the major allergen identified in Dermatophagoides farinae. The objective of this study was to predict the B and T cell epitopes of Der f 25. In the present study, we analyzed the physiochemical properties, function motifs and domains, and structural-based detailed features of Der f 25 and predicted the B cell linear epitopes of Der f 25 by DNAStar protean system, BPAP, and BepiPred 1.0 server and the T cell epitopes by NetMHCIIpan-3.0 and NetMHCII-2.2. As a result, the sequence and structure analysis identified that Der f 25 belongs to the triosephosphate isomerase family and exhibited a triosephosphate isomerase pattern (PS001371. Eight B cell epitopes (11–18, 30–35, 71–77, 99–107, 132–138, 173–187, 193–197, and 211–224 and five T cell epitopes including 26–34, 38–54, 66–74, 142–151, and 239–247 were predicted in this study. These results can be used to benefit allergen immunotherapies and reduce the frequency of mite allergic reactions.

  2. Eucalyptus oil-loaded microcapsules grafted to cotton fabrics for acaricidal effect against Dermatophagoides farinae.

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    Kim, Joo Ran

    2017-05-01

    The purpose of this study was to develop acaricidal cotton fabrics grafted with eucalyptus oil-loaded microcapsules (EOMCs) produced from green resources. EOMCs showed a broad size distribution between 0.5 and 6.5 μm, and had the average diameter 1.8 μm. EOMCs exhibited nonporous spherical shapes and individually remained on cotton fibres. Through AATCC mortality tests against house dust mites (HDMs) (Dermatophagoides farinae), the treated cotton fabric containing EOMCs resulted in 98.7% mortality. Acaricidal efficiency was due to the large amount of oxygenated monoterpene, 1,8-eucalyptol (75.8%) and hydroxylated monoterpenes such as α-terpineol (3.6%), terpinen-4-ol (0.4%) and linalool (0.3%) found in eucalyptus oil. These compounds are effective in enhancing penetration into HDMs. EOMCs produced from safe and natural sources can serve as a replacement for synthetic acaricides in controlling the population of HDM, leading to positive impacts on the human health and environment.

  3. Allergic asthma induced in rhesus monkeys by house dust mite (Dermatophagoides farinae).

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    Schelegle, E S; Gershwin, L J; Miller, L A; Fanucchi, M V; Van Winkle, L S; Gerriets, J P; Walby, W F; Omlor, A M; Buckpitt, A R; Tarkington, B K; Wong, V J; Joad, J P; Pinkerton, K B; Wu, R; Evans, M J; Hyde, D M; Plopper, C G

    2001-01-01

    To establish whether allergic asthma could be induced experimentally in a nonhuman primate using a common human allergen, three female rhesus monkeys (Macaca mulatta) were sensitized with house dust mite (Dermatophagoides farinae) allergen (HDMA) by subcutaneous injection, followed by four intranasal sensitizations, and exposure to allergen aerosol 3 hours per day, 3 days per week for up to 13 weeks. Before aerosol challenge, all three monkeys skin-tested positive for HDMA. During aerosol challenge with HDMA, sensitized monkeys exhibited cough and rapid shallow breathing and increased airway resistance, which was reversed by albuterol aerosol treatment. Compared to nonsensitized monkeys, there was a fourfold reduction in the dose of histamine aerosol necessary to produce a 150% increase in airway resistance in sensitized monkeys. After aerosol challenge, serum levels of histamine were elevated in sensitized monkeys. Sensitized monkeys exhibited increased levels of HDMA-specific IgE in serum, numbers of eosinophils and exfoliated cells within lavage, and elevated CD25 expression on circulating CD4(+) lymphocytes. Intrapulmonary bronchi of sensitized monkeys had focal mucus cell hyperplasia, interstitial infiltrates of eosinophils, and thickening of the basement membrane zone. We conclude that a model of allergic asthma can be induced in rhesus monkeys using a protocol consisting of subcutaneous injection, intranasal instillation, and aerosol challenge with HDMA.

  4. Cloning, expression, and analysis of the group 2 allergen from Dermatophagoides farinae from China

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    Cui Yu-bao

    2010-12-01

    Full Text Available To obtain the recombinant group 2 allergen product of Dermatophagoides farinae (Der f 2, the Der f 2 gene was synthesized by RT-PCR. The full-length cDNA comprised 441 nucleotides and was 99.3% identical to the reference sequence (GenBank AB195580. The cDNA was bound to vector pET28a to construct plasmid pET28a(+-Der f 2, which was transformed into E. coli BL21 and induced by IPTG. SDS-PAGE showed a specific band of about 14kDa in the hole cell lysate. s estiated by chroatography, about 3.86 g of the recobinant product as obtained, which conjugated with serum IgE from asthmatic children. The protein had a signal peptide of 17 amino acids. Its secondary structure comprised an alpha helix (19.86%, an extended strand (30.82%, and a random coil (49.32%. The subcellular localization of this allergen was predicted to be at mitochondria. Furthermore, its function was shown to be associated with an MD-2-related lipid-recognition (ML domain. The results of this study provide a solid foundation for large-scale production of the allergen for clinical diagnosis and treatent of allergic disorders.Com a finalidade de obter o produto recombinante do alergeno grupo 2 do Dermatophagoides farinae (Der f2, o gene Der f2 foi sintetizado por RT-PCR. O cDNA continha 441 nucleotídeos e era idêntico em 99,3% à sequência de referência (GenBank AB195580. O cDNA foi ligado ao vetor pET28a para construir o plasmídeo pET28a(+-Der f2, o qual foi introduzido por transformação em E. coli BL21 e induzido por IPTG. Em SDS-PAGE foi vista mia banda específica de 14 kDa no lisado celular. Conforme estimado por cromatografia, cerca de 3,86 mg do produto recombinante foi obtido, que reagia com IgE sérica de crianças asmáticas. A proteína continha um peptídeo sinal de 17 amino ácidos. Sua estrutura secundária consistia de uma alfa hélice (19,86%, uma fita estendida (30,82%, e uma sequência randômica (49,32%. A localização subcelular desse alergeno foi predita

  5. New Uropodina species and records from Malaysia (Acari: Mesostigmata)

    Czech Academy of Sciences Publication Activity Database

    Kontschán, J.; Starý, Josef

    2012-01-01

    Roč. 58, č. 2 (2012), s. 177-192 ISSN 1217-8837 Institutional research plan: CEZ:AV0Z60660521 Keywords : Acari * new records * new species * Malaysia Subject RIV: EH - Ecology, Behaviour Impact factor: 0.472, year: 2012

  6. Two new Uropodina species from Ethiopia (Acari: Mesostigmata)

    Czech Academy of Sciences Publication Activity Database

    Kontschán, J.; Starý, Josef

    2013-01-01

    Roč. 54, č. 1 (2013), s. 49-56 ISSN 1681-5556 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Acari * Uropodina * Afrotropical * Ethiopia * new species * new synonymy Subject RIV: EG - Zoology Impact factor: 0.500, year: 2013

  7. Biological control of Eotetranychus lewisi and Tetranychus urticae (Acari: Tetranychidae) on strawberry by four phytoseiids (Acari: Phytoseiidae).

    Science.gov (United States)

    Howell, Anna D; Daugovish, Oleg

    2013-02-01

    The spider mite, Eotetranychus lewisi (McGregor) (Acari: Tetranychidae), is a new emerging pest in California commercial strawberries. The predatory mite Phytoseiulus persimilis (Athias-Henriot) (Acari: Phytoseiidae), typically used for biocontrol of Tetranychus urticae (Koch) (Acari: Tetranychidae), provided growers little to no control of E. lewisi. Four commonly used phytoseiid predatory mites: P. persimilis, Neoseiulus californicus (McGregor), N. fallacis (Garman), and Amblyseius andersoni (Chant), were used in lab studies to investigate which is best at managing E. lewisi populations. We als o investigated t he interactions between T. urticae and E. lewisi and in relation to phytoseiid efficiency given the potential for indirect effects of biocontrol. When E. lewisi and T. urticae are present on the same leaf, T. urticae populations increase and begin displacing E. lewisi. P. persimilis did not feed on E. lewisi, but the other three predatory mites consumed the spider mites and lowered their populations. When both E. lewisi and T. urticae are present on the same leaf, N. fallacis and A. andersoni fed on both types of mites equally and were capable of decreasing both populations. N. californicus fed on E. lewisi first and decreased its population, but allowed T. urticae populations to increase. P. persimilis may be insufficient at controlling E. lewisi and its use may instead enhance E. lewisi populations.

  8. New records of water mites (Acari: Hydrachnidia, Halacaroidea) from Patagonia (Chile)

    NARCIS (Netherlands)

    Pešić, V.; Smit, H.; Datry, T.

    2010-01-01

    New records of water mite species (Acari: Hydrachnidia, Halacaroidea) from Patagonia (Southern Chile) are reported. Four species, Anisitsiellides australis Smit, 2002, Peregrinacarus falklandensis Bartsch, 2001, Lobohalacarus weberi (Romijn & Viets, 1924) and Soldanellonyx monardi Walter, 1919 are

  9. Temperature-dependent development and reproductive traits of Tetranychus macfarlanei (Acari: Tetranychidae)

    DEFF Research Database (Denmark)

    Ullah, Mohammad Shaef; Haque, Md. Ahsanul; Nachman, Gösta

    2012-01-01

    Development and reproductive traits of Tetranychus macfarlanei Baker & Pritchard (Acari: Tetranychidae) were investigated on kidney bean, Phaseolus vulgaris L., at eleven constant temperatures. Tetranychus macfarlanei was able to develop and complete its life cycle at temperatures ranging from 17...

  10. Three new .i.Trachyuropoda./i. (Acari: Uropodina: Trachyuropodidae) species from the Neotropical region

    Czech Academy of Sciences Publication Activity Database

    Kontschán, J.; Starý, Josef

    2013-01-01

    Roč. 37, č. 1 (2013), s. 7-14 ISSN 1300-0179 Institutional support: RVO:60077344 Keywords : Acari * Uropodina * Trachyuropoda * Galápagos Islands Subject RIV: EG - Zoology Impact factor: 0.585, year: 2013

  11. First report of Aculops lycopersici (Tryon, 1917 (Acari: Eriophyidae on Pepino in Turkey

    Directory of Open Access Journals (Sweden)

    Rana Akyazi

    2012-12-01

    Full Text Available The tomato russet mite, Aculops lycopersici (Tryon, 1917 (Acari: Eriophyidae is reported for the first time on Pepino (Solanum muricatum Aiton in Ordu and Samsun provinces in Turkey.

  12. A new genus and species Mangalaus krishianusandhanus (Acari: Eriophyidae) from India

    Science.gov (United States)

    Mangalaus ikrishianusandhanus n. gen., n. sp., (Acari: Prostigmata: Eriophyoidea), collected from erineum on the underside of leaves of Cordia dichotoma (Boraginaceae) is described and illustrated from specimens collected at the Indian Agricultural Research Institute (IARI) in New Delhi, India....

  13. Contact and fumigant toxicity of Armoracia rusticana essential oil, allyl isothiocyanate and related compounds to Dermatophagoides farinae.

    Science.gov (United States)

    Yun, Yeon-Kyeong; Kim, Hyun-Kyung; Kim, Jun-Ran; Hwang, Kumnara; Ahn, Young-Joon

    2012-05-01

    The toxicity to adult Dermatophagoides farinae of allyl isothiocyanate identified in horseradish, Armoracia rusticana, oil and another 27 organic isothiocyanates was evaluated using contact + fumigant and vapour-phase mortality bioassays. Results were compared with those of two conventional acaricides, benzyl benzoate and dibutyl phthalate. Horseradish oil (24 h LC(50), 1.54 µg cm(-2)) and allyl isothiocyanate (2.52 µg cm(-2)) were highly toxic. Benzyl isothiocyanate (LC(50) , 0.62 µg cm(-2)) was the most toxic compound, followed by 4-chlorophenyl, 3-bromophenyl, 3,5-bis(trifluoromethyl)phenyl, cyclohexyl, 2-chlorophenyl, 4-bromophenyl and 2-bromophenyl isothiocyanates (0.93-1.41 µg cm(-2)). All were more effective than either benzyl benzoate (LC(50) , 4.58 µg cm(-2)) or dibutyl phthalate (24.49 µg cm(-2)). The structure-activity relationship indicates that types of functional group and chemical structure appear to play a role in determining the isothiocyanate toxicities to adult D. farinae. In the vapour-phase mortality bioassay, these isothiocyanates were consistently more toxic in closed versus open containers, indicating that their mode of delivery was, in part, a result of vapour action. In the light of global efforts to reduce the level of highly toxic synthetic acaricides in indoor environments, the horseradish oil-derived compounds and the isothiocyanates described herein merit further study as potential acaricides for the control of house dust mite populations as fumigants with contact action. Copyright © 2011 Society of Chemical Industry.

  14. Chlorella vulgaris Attenuates Dermatophagoides Farinae-Induced Atopic Dermatitis-Like Symptoms in NC/Nga Mice

    Science.gov (United States)

    Kang, Heerim; Lee, Chang Hyung; Kim, Jong Rhan; Kwon, Jung Yeon; Seo, Sang Gwon; Han, Jae Gab; Kim, Byung Gon; Kim, Jong-Eun; Lee, Ki Won

    2015-01-01

    Atopic dermatitis (AD) is a chronic and inflammatory skin disease that can place a significant burden on quality of life for patients. AD most frequently appears under the age of six and although its prevalence is increasing worldwide, therapeutic treatment options are limited. Chlorella vulgaris (CV) is a species of the freshwater green algae genus chlorella, and has been reported to modulate allergy-inducible factors when ingested. Here, we examined the effect of CV supplementation on AD-like symptoms in NC/Nga mice. CV was orally administrated for six weeks while AD-like symptoms were induced via topical application of Dermatophagoides farinae extract (DFE). CV treatment reduced dermatitis scores, epidermal thickness, and skin hydration. Histological analysis also revealed that CV treatment reduced DFE-induced eosinophil and mast cell infiltration into the skin, while analysis of serum chemokine levels indicated that CV treatment downregulated thymus- and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) levels. In addition, CV treatment downregulated mRNA expression levels of IL-4 and IFN-γ. Taken together, these results suggest that CV extract may have potential as a nutraceutical ingredient for the prevention of AD. PMID:26404252

  15. Chlorella vulgaris Attenuates Dermatophagoides Farinae-Induced Atopic Dermatitis-Like Symptoms in NC/Nga Mice

    Directory of Open Access Journals (Sweden)

    Heerim Kang

    2015-09-01

    Full Text Available Atopic dermatitis (AD is a chronic and inflammatory skin disease that can place a significant burden on quality of life for patients. AD most frequently appears under the age of six and although its prevalence is increasing worldwide, therapeutic treatment options are limited. Chlorella vulgaris (CV is a species of the freshwater green algae genus chlorella, and has been reported to modulate allergy-inducible factors when ingested. Here, we examined the effect of CV supplementation on AD-like symptoms in NC/Nga mice. CV was orally administrated for six weeks while AD-like symptoms were induced via topical application of Dermatophagoides farinae extract (DFE. CV treatment reduced dermatitis scores, epidermal thickness, and skin hydration. Histological analysis also revealed that CV treatment reduced DFE-induced eosinophil and mast cell infiltration into the skin, while analysis of serum chemokine levels indicated that CV treatment downregulated thymus- and activation-regulated chemokine (TARC and macrophage-derived chemokine (MDC levels. In addition, CV treatment downregulated mRNA expression levels of IL-4 and IFN-γ. Taken together, these results suggest that CV extract may have potential as a nutraceutical ingredient for the prevention of AD.

  16. The Drinking Effect of Hydrogen Water on Atopic Dermatitis Induced by Dermatophagoides farinae Allergen in NC/Nga Mice

    Directory of Open Access Journals (Sweden)

    Rosa Mistica C. Ignacio

    2013-01-01

    Full Text Available Hydrogen water (HW produced by electrolysis of water has characteristics of extremely low oxidation-reduction potential (ORP value and high dissolved hydrogen (DH. It has been proved to have various beneficial effects including antioxidant and anti-inflammatory effects; however, HW effect on atopic dermatitis (AD, an inflammatory skin disorder, is poorly documented. In the present study, we examined the immunological effect of drinking HW on Dermatophagoides farinae-induced AD-like skin in NC/Nga mice. Mice were administered with HW and purified water (PW for 25 days. We evaluated the serum concentration of pro-inflammatory (TNF-α, Th1 (IFN-γ, IL-2, and IL-12p70, Th2 (IL-4, IL-5, and IL-10, and cytokine expressed by both subsets (GM-CSF to assess their possible relationship to the severity of AD. The serum levels of cytokines such as IL-10, TNF-α, IL-12p70, and GM-CSF of mice administered with HW was significantly reduced as compared to PW group. The results suggest that HW affects allergic contact dermatitis through modulation of Th1 and Th2 responses in NC/Nga mice. This is the first note on the drinking effect of HW on AD, clinically implying a promising potential remedy for treatment of AD.

  17. Sensitivity to House Dust Mites Allergens with Atopic Asthma and Its Relationship with CD14 C(-159T) Polymorphism in Patients of West Bengal, India.

    Science.gov (United States)

    Ghosh, Amlan; Dutta, Shampa; Podder, Sanjoy; Mondal, Priti; Laha, Arghya; Saha, Nimai Chandra; Moitra, Saibal; Saha, Goutam Kumar

    2018-01-10

    India is the home to around 15-20 million asthmatics, and asthma prevalence is increasing in Indian metropolitan area, including Kolkata, West Bengal. Complex interactions of genetic and environmental factors are involved in asthma. Genome-wide search for susceptible loci regulating IgE response (atopy) have identified a candidate gene CD14 which is most important in the context of allergic responses of respiratory system. This study was aimed to investigate the role of house dust and house dust mites in development of bronchial asthma and to explore the possible association of candidate gene CD14 with disease manifestation among Kolkata patient population. Skin-prick test was done among 950 asthmatic patients against 8 aeroallergens, including house dust and house dust mites and total serum IgE and allergen-specific IgE were measured. Polymerase chain reaction-restriction fragment length polymorphism was done in patients and nonasthmatic control (n = 255 in each) to characterize a functional polymorphism, C(-159)T, of CD14, a positional candidate gene for allergy. We identified house dust as the most common aeroallergen sensitizer among atopic patients in Kolkata followed by Dermatophagoides pteronyssinus and Dermatophagoides farinae Hughes (Acari: Pyroglyphidae) mites. Patient's sera contain significantly higher IgE level than that of control. Allergen-specific IgE antibody test revealed that 76.36% patients had specific IgE antibody against D. pteronyssinus mite. There was a significant difference in the distribution of alleles and genotypes for CD14 polymorphism with an increase in disease severity. So, in Kolkata, house dust mite is a common aeroallergen and D. pteronyssinus is predominant among mites. The present study revealed that bronchial asthma has a genetic background. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  18. Hataedock treatment has preventive therapeutic effects for atopic dermatitis through skin barrier protection in Dermatophagoides farinae-induced NC/Nga mice.

    Science.gov (United States)

    Cha, Ho-Yeol; Ahn, Sang-Hyun; Cheon, Jin-Hong; Park, Sun-Young; Kim, Kibong

    2017-07-12

    Hataedock treatment is traditionally used for the purpose of preventing the future skin disease by feeding herbal extracts to the newborn in traditional Chinese and Korean medicine. This study investigated the preventive therapeutic effects of Hataedock (HTD) treatment for atopic dermatitis (AD) through skin barrier protection in Dermatophagoides farinae-induced NC/Nga mice. To the HTD treatment group, the extract of Coptis japonica Makino and Glycyrrhiza uralensis Fischer, which analyzed with High Performance Liquid Chromatography (HPLC)-fingerprint for quality consistency, was administered orally to the 3-week-old mice before inducing AD. After that, Dermatophagoides farinae was applied except the control group to induce AD-like skin lesions. We confirmed the effects of HTD on morphological changes, protection of skin barrier, regulation of Th2 differentiation, inflammation regulation and induction of apoptosis through histochemistry, immunohistochemistry, and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. HTD effectively reduced edema, angiogenesis and skin lesion. HTD also increased the levels of liver X receptor (LXR) and filaggrin but decreased the level of protein kinase C (PKC) (pprotection of skin barrier. Therefore, HTD may have potential applications for alternative and preventive treatment in the management of AD. Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.

  19. Impact of sulfur on density of Tetranychus pacificus (Acari: Tetranychidae) and Galendromus occidentalis (Acari: Phytoseiidae) in a central California vineyard.

    Science.gov (United States)

    Costello, Michael J

    2007-01-01

    Sulfur is the oldest and most widely used fungicide in the vineyards of California, where it is used for control of powdery mildew (Uncinula necator [Schw.] Burr). For decades, sulfur use has been associated with outbreaks of Tetranychus pacificus McGregor (Acari: Tetranychidae) on cultivated grapes in the San Joaquin Valley. I undertook large-scale field studies to test this association, to evaluate the impact of sulfur on Galendromus occidentalis (Nesbit) (Acari: Phytoseiidae), a major predator of T. pacificus, and to determine if timing of sulfur applications with respect to grape bloom has an impact on T. pacificus density. The studies took place in a 32 ha vineyard in Fresno County, and all fungicide applications were made with commercial-scale equipment. In 1998 a 'high sulfur' treatment, a combination of wettable sulfur and sulfur dust, was compared to 'low sulfur,' in which demethylation inhibitor (DMI) fungicides partially substituted for sulfur. In 1999 treatments were 'sulfur,' 'DMI,' 'sulfur pre-bloom' (here sulfur was applied prior to grape bloom, in late May, and then DMIs were applied until mid-season) and 'sulfur post-bloom' (the reverse of 'sulfur pre-bloom'). In each year, the T. pacificus population increase came after the end of fungicide applications, and results clearly show a relationship between sulfur use and T. pacificus density. In 1998, mean T. pacificus density was 2.7 times higher and mean G. occidentalis density 2.5 times higher in 'high sulfur' compared to 'low sulfur.' In 1999, the highest T. pacificus counts were in the 'sulfur' and 'sulfur pre-bloom' treatments, 4.8 times higher than 'sulfur post-bloom' and 2 times higher than 'DMIs.' Density of G. occidentalis was 2.3 times as high in 'sulfur' or 'sulfur pre-bloom' than 'DMIs.' The predator/prey ratio was not significantly different among treatments in 1998, but in 1999 it was highest in the 'sulfur pre-bloom' treatment. In 1999, density of Homeopronematus anconai (Baker) (Acari

  20. Mites (acari) infesting commensal rats in Suez Canal zone, Egypt.

    Science.gov (United States)

    el Kady, G A; Shoukry, A; Ragheb, D A; el Said, A M; Habib, K S; Morsy, T A

    1995-08-01

    Mites are arthropods distinguished from ticks by usually being microscopical in size and have a hypostome unarmed with tooth-like anchoring processes. They are group in a number of suborders, each with super-families and families including many genera of medical and economic importance. In this paper, commensal rodents (Rattus norvegicus, R. r. alexandrinus and R. r. frugivorous) were surveyed in the Suez Canal Zone for their acari ectoparasites. Four species of mites were recovered. In a descending order of mite indices, they were Eulaelaps stabularis (4.83 on 6 rats), Laelaps nuttalli (3.11 on 27 rats), Ornithonyssus bacoti (1.66 on 9 rats) and Dermanyssus gallinae (0.66 on 24 rats). The overall mite indices in the three governorates were 3.66 in Suez, 2.82 in Ismailia and zero in Port Said. The medical and economic importance of the mites were discussed.

  1. Further studies on South African plants: Acaricidal activity of organic plant extracts against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)

    CSIR Research Space (South Africa)

    Wellington, Kevin W

    2017-01-01

    Full Text Available -1 Veterinary Parasitology, vol. 234: 10-12 Further studies on South African plants: Acaricidal activity of organic plant extracts against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) Wellington, KW Leboho, T Sakong, BM Adenubi, OT Eloff, JN...

  2. Primeiro registro de Tetranychus urticae (Acari: Tetranychidae em mudas de teca no Brasil

    Directory of Open Access Journals (Sweden)

    Alexandre dos Santos

    2014-07-01

    Full Text Available O ácaro fitófago Tetranychus urticae (Kock, 1836 (Acari: Tetranychidae foi relatado e suas injúrias caracterizadas pela primeira vez em viveiro de produção mudas de teca (Tectona grandis no Brasil.

  3. New records of Acari from the sub-Antarctic Prince Edward Islands

    NARCIS (Netherlands)

    Marshall, D.J.; Gremmen, N.J.M.; Coetzee, L.; Oconnor, B.M.; Pugh, P.J.A.; Theron, P.D.; Ueckermann, E.A.

    1999-01-01

    Sixty species of Acari are recorded from the sub-Antarctic Marion and Prince Edward Islands (the Prince Edward archipelago). Twenty of the 45 species collected on recent expeditions are new and currently undescribed. Other new taxa include a family of Mesostigmata, four new genera, and the first

  4. Five New Records of Raphignathoid Mites (Acari: Raphignathoidea) from Poland/ Pięć Nowych Gatunków Roztoczy Z Nadrodziny Raphignathoidea (Acari) Stwierdzonych W Faunie Polski

    OpenAIRE

    Doðan Salih; Sevsay Sevgi; Mąkol Joanna; Zeytun Erhan; Buğa Evren

    2014-01-01

    Pięć nowych gatunków roztoczy (Acari: Raphignathoidea) zostało odnoto- wanych jako nowe dla fauny Polski: trzy z rodziny Stigmaeidae, Eustigmaeus rhodomela (Koch), Mediolata obtecta Dónel et Doóan, Stigmaeus glabrisetus Summers, jeden z rodziny Cryptognathidae, Favognathus cucurbita (Berlese), i jeden z rodziny Barbutiidae, Barbuda anguineus (Berlese). Ostatni z wymienio- nych jest jednocześnie pierwszym stwierdzeniem przedstawiciela rodziny Barbutiidae w Polsce.

  5. Five New Records of Raphignathoid Mites (Acari: Raphignathoidea from Poland/ Pięć Nowych Gatunków Roztoczy Z Nadrodziny Raphignathoidea (Acari Stwierdzonych W Faunie Polski

    Directory of Open Access Journals (Sweden)

    Doðan Salih

    2014-12-01

    Full Text Available Pięć nowych gatunków roztoczy (Acari: Raphignathoidea zostało odnoto- wanych jako nowe dla fauny Polski: trzy z rodziny Stigmaeidae, Eustigmaeus rhodomela (Koch, Mediolata obtecta Dónel et Doóan, Stigmaeus glabrisetus Summers, jeden z rodziny Cryptognathidae, Favognathus cucurbita (Berlese, i jeden z rodziny Barbutiidae, Barbuda anguineus (Berlese. Ostatni z wymienio- nych jest jednocześnie pierwszym stwierdzeniem przedstawiciela rodziny Barbutiidae w Polsce.

  6. Oviposition behaviour of the soil mite Pergamasus brevicornis (Acari: Parasitidae).

    Science.gov (United States)

    Marquardt, Tomasz; Faleńczyk-Koziróg, Katarzyna; Kaczmarek, Sławomir

    2013-07-01

    We observed the oviposition behaviour of the soil mite Pergamasus brevicornis Berlese (Acari: Parasitidae) using continuous video-monitoring. Oviposition consisted of six sequential phases. The first phase (I) involved inspection of the substrate. In the second phase (II) there were rhythmic movements of the first pair of legs and slight reciprocating movements of the body. The third (III) was a resting phase. In the fourth phase (IV) the gnathosoma was lowered and the body was raised. In the next phase (V) there were two sub-phases. During the first (Va), the female held the egg below the gnathosoma. In the second sub-phase (Vb), the gnathosoma moved up holding the egg, which was then placed on the substrate. The last phase (VI) involved intense 'cleaning' movements of the chelicerae and palps. During Va a protective external eggshell structure is gradually formed, involving a phase where the egg shell is sticky. After moving the egg to the substrate, the female freed her palps and chelicerae from the sticky egg shell and cleaned her gnathosomal appendages. Phases II-V took on average 207 ± 69 s.

  7. Catalogue of ptyctimous mites (Acari, Oribatida) of the world.

    Science.gov (United States)

    NiedbaŁa, Wojciech; Liu, Dong

    2018-03-11

    As important representatives of Oribatida (Acari), ptyctimous mites comprise more than 1400 described species in 40 genera and subgenera, with nearly cosmopolitan distribution except for the Arctic and Antarctic Regions. They are capable of folding the aspidosoma under the opisthosoma to protect their appendages, and are primarily soil and litter inhabitants, feeding on fungi and decaying plant remains with various levels of specificity. Our purpose was to provide a detailed catalogue of all known ptyctimous mite species in the world with information of distribution, taxonomic issues and some remarks. Data of known juvenile  instars of ptyctimous mites which were not included in Norton Ermilov (2014) were added. We hope that our catalogue with bibliography will be helpful in taxonomic and ecological studies.        The catalogue presents taxonomic information and geographic distribution of 1431 known species of the world belonging to 42 genera and eight families (not including data of genus and species inquirenda, nomina nuda and species without author name). Among them, 261 species are listed as synonyms, 43 species inquirenda, nine homonyms, 17 new synonyms, one new subgenus Mahuntritia subgenus nov. and three new names are included in the catalogue.

  8. Theobroma cacao extract attenuates the development of Dermatophagoides farinae-induced atopic dermatitis-like symptoms in NC/Nga mice.

    Science.gov (United States)

    Kang, Heerim; Lee, Chang Hyung; Kim, Jong Rhan; Kwon, Jung Yeon; Son, Myoung-Jin; Kim, Jong-Eun; Lee, Ki Won

    2017-02-01

    Cacao beans from Theobroma cacao are an abundant source of polyphenols, particularly flavonoids. Previous studies demonstrated that cacao flavanols decrease pro-inflammatory cytokines resulting in the alleviation of allergic symptoms. We sought to investigate the effects of cacao extract (CE) on Dermatophagoides farinae extract (DFE)-induced atopic dermatitis (AD)-like symptoms. CE attenuated DFE-induced AD-like symptoms as assessed by skin lesion analyses, dermatitis score, and skin thickness. Histopathological analysis revealed that CE suppressed DFE-induced immune cell infiltration into the skin. These observations occurred concomitantly with the downregulation of inflammatory markers including serum immunoglobulin (Ig) E, chemokine; thymus and activation-regulated chemokine and macrophage-derived chemokine as well as the skin-derived cytokines interleukin (IL)-4, IL-5, and interferon-γ. CE also significantly alleviated transepidermal water loss and increased skin hydration. These results suggest that CE, a natural phytochemical-rich food, has potential therapeutic efficacy for the treatment of AD. Copyright © 2016. Published by Elsevier Ltd.

  9. 7,3′,4′-Trihydroxyisoflavone Ameliorates the Development of Dermatophagoides farinae-Induced Atopic Dermatitis in NC/Nga Mice

    Directory of Open Access Journals (Sweden)

    Bo-Bae Kim

    2013-01-01

    Full Text Available Atopic dermatitis is an inflammatory and chronically relapsing skin disorder that commonly occurs in children; the number of atopic dermatitis patients is increasing. The cause and mechanism of atopic dermatitis have not been defined clearly, although many studies are ongoing. Epidemiological studies suggest that soybean and its isoflavones have immunoregulatory activities. Here, we report that 7,3′,4′-trihydroxyisoflavone (7,3′,4′-THIF, a major metabolite of daidzin, effectively inhibited lipopolysaccharide (LPS-induced nitric oxide (NO, tumor necrosis factor (TNF-α, and interleukin (IL-6 production in RAW 264.7 cells, and also reduced β-hexosaminidase secretion in RBL-2H3 cells. Moreover, 7,3′,4′-THIF significantly reduced scratching time, transepidermal water loss, and mast cell infiltration. It also decreased protease-activated receptor (PAR-2 and IL-4 expression and increased filaggrin expression in skin lesions of NC/Nga mice. These results suggest that 7,3′,4′-THIF improves Dermatophagoides farina body extract-induced atopic dermatitis in NC/Nga mice.

  10. A large parasitengonid mite (Acari, Erythraeoidea from the Early Cretaceous Crato Formation of Brazil

    Directory of Open Access Journals (Sweden)

    J. A. Dunlop

    2007-08-01

    Full Text Available A new large, fossil mite (Arachnida: Acari, Pararainbowia martilli n. gen. n. sp., is described from the Early Cretaceous (Aptian Crato Formation from Ceará State, Brazil. It is assigned to the Cohort Parasitengona and the superfamily Erythraeoidea, some extant members of which can reach up to seven millimetres in body length. Given that doubts have been raised about the identity of putative Crato feather mite eggs, this new fossil represents the first unequivocal record of Acari from the Crato Formation, the first non-amber record of an erythraeoid mite and the oldest named example of this superfamily. Fossil erythraeoids from Mesozoic and Tertiary ambers are briefly reviewed – including a widely overlooked Late Cretaceous species – with comments on Mesozoic mites in general. Thirteen Baltic amber erythraeoids have been formally described, but much unstudied material from various amber sources remains. Ein neues großes Milbenfossil (Arachnida: Acari, Pararainbowia martilli n. gen. n. sp., wird aus der Crato Formation (Unterkreide, Aptium des Ceará Gebietes in Brasilien beschrieben. Es wird der Kohorte Parasitengona und der Überfamilie Erythraeoidea zugeordnet; die modernen Vertreter erreichen eine Körperlänge bis zu sieben mm. Weil die Identität von Federmilbeneiern aus der Crato Formation in Frage gestellt wurde, ist dieser Neufund der erste klare Hinweis von Acari aus der Crato Formation. Es ist die erste erythraeoide Milbe, die nicht aus dem Bernstein stammt sowie das älteste genannte Beispiel dieser Überfamilie. Fossile erythraeoide Milben aus dem Bernstein des Mesozoikum und des Tertiärs werden kurz zusammengefasst – u. a. eine weitgehend übersehene Art aus der Oberkreide – mit allgemeinen Anmerkungen zu den mesozoischen Milben. Dreizehn erythraeoide Milbenarten sind aus dem baltischen Bernstein genannt und beschrieben worden, aber weiteres unbearbeitetes Material von verschiedenen Bernstein-Fundpunkten liegt noch vor

  11. Acaricidal and repellent activities of essential oil of Eucalyptus globulus against Dermanyssus gallinae (Acari: Mesostigmata)

    OpenAIRE

    Dehghani-Samani Amir; Madreseh-Ghahfarokhi Samin; Dehghani-Samani Azam; Pirali-Kheirabadi Khodadad

    2015-01-01

    Introduction: By considering an increase in drug resistance against red mites, finding the nonchemical herbal acaricide against Dermanyssus gallinae (De Geer) (Acari: Mesostigmata) is necessary to kill them and to reduce the chemical resistance against chemical acaricides in this specie. Dermanyssus gallinae is a potential vector of the causal agent of several viral diseases such as Equine encephalitis and St. Louis encephalitis. It can be a vector of bacteria such as Salmonella spp., Mycobac...

  12. . 8~~,

    African Journals Online (AJOL)

    dence of 'climate asthma' to the factor of relarive humidity. The mite most commonly found in the house dusts in. South Africa is Dermatophagoides pteronyssinus. Other mites found include Euroglyphus maynei, Chortoglyphus domesticus and orher species. Dermatophagoides farinae mites were recovered in scanty ...

  13. Marine water mites (Acari: Hydrachnidia: Pontarachnidae) from Taiwan, Korea and India, with the first description of the male of Pontarachna australis Smit, 2003

    Digital Repository Service at National Institute of Oceanography (India)

    Pesic, V.; Chatterjee, T.; Chan, B.K.K.; Ingole, B.S.

    he hitherto unknown male of the marine water mite Pontarachna australis Smit, 2003 (Acari: Hydrachnidia: Pontarachnidae) is described and recorded from Taiwan for the first time. Another marine water mite, Litarachna denhami (Lohmann, 1909...

  14. The predatory mite Neoseiulus womersleyi (Acari: Phytoseiidae) follows extracts of trails left by the two-spotted spider mite Tetranychus urticae (Acari: Tetranychidae).

    Science.gov (United States)

    Shinmen, Tsubasa; Yano, Shuichi; Osakabe, Mh

    2010-10-01

    As it walks, the two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae) spins a trail of silk threads, that is followed by the predatory mite, Neoseiulus womersleyi Schicha (Acari: Phytoseiidae). Starved adult female N. womersleyi followed T. urticae trails laid down by five T. urticae females but did not follow a trail of one T. urticae female, suggesting that the amount of spun threads and their chemical components should correlate positively with the number of T. urticae individuals. To examine whether chemical components of T. urticae trails are responsible for the predatory mite's trail following, we collected separate T. urticae threads from the exuviae and eggs, and then washed the threads with methanol to separate chemical components from physical attributes of the threads. Female N. womersleyi did not follow T. urticae trails that had been washed with methanol but contained physical residues, but they did follow the direction to which the methanol extracts of the T. urticae trails was applied. These results suggest that the predatory mite follows chemical, not physical, attributes of T. urticae trails.

  15. Ticks (Acari: Ixodida) on wild carnivores in Brazil.

    Science.gov (United States)

    Labruna, Marcelo B; Jorge, Rodrigo S P; Sana, Dênis A; Jácomo, Anah Tereza A; Kashivakura, Cyntia K; Furtado, Mariana M; Ferro, Claudia; Perez, Samuel A; Silveira, Leandro; Santos, Tarcísio S; Marques, Samuel R; Morato, Ronaldo G; Nava, Alessandra; Adania, Cristina H; Teixeira, Rodrigo H F; Gomes, Albério A B; Conforti, Valéria A; Azevedo, Fernando C C; Prada, Cristiana S; Silva, Jean C R; Batista, Adriana F; Marvulo, Maria Fernanda V; Morato, Rose L G; Alho, Cleber J R; Pinter, Adriano; Ferreira, Patrícia M; Ferreira, Fernado; Barros-Battesti, Darci M

    2005-01-01

    The present study reports field data of ticks infesting wild carnivores captured from July 1998 to September 2004 in Brazil. Additional data were obtained from one tick collection and from previous published data of ticks on carnivores in Brazil. During field work, a total of 3437 ticks were collected from 89 Cerdocyon thous (crab-eating fox), 58 Chrysocyon brachyurus (maned wolf), 30 Puma concolor (puma), 26 Panthera onca (jaguar), 12 Procyon cancrivorus (crab-eating raccoon), 4 Speothos venaticus (bush dog), 6 Pseudalopex vetulus (hoary fox), 6 Nasua nasua (coati), 6 Leopardus pardalis (ocelot), 2 Leopardus tigrinus (oncilla), 1 Leopardus wiedii (margay), 1 Herpailurus yagouaroundi (jaguarundi), 1 Oncifelis colocolo (pampas cat), 1 Eira barbara (tayara), 1 Galictis vittata (grison), 1 Lontra longicaudis (neotropical otter), and 1 Potus flavus (kinkajou). Data obtained from the Acari Collection IBSP included a total of 381 tick specimens collected on 13 C. thous, 8 C. brachyurus, 3 P. concolor, 10 P. onca, 3 P. cancrivorus, 4 N. nasua, 1 L. pardalis, 1 L. wiedii, 4 H. yagouaroundi, 1 Galictis cuja (lesser grison), and 1 L. longicaudis. The only tick-infested carnivore species previously reported in Brazil, for which we do not present any field data are Pseudalopex gymnocercus (pampas fox), Conepatus chinga (Molina's hog-nosed skunk), and Conepatus semistriatus (striped hog-nosed skunk). We report the first tick records in Brazil on two Felidae species (O. colocolo, H. yagouaroundi), two Canidae species (P. vetulus, S. venaticus), one Procyonidae species (P. flavus) and one Mustelidae (E. barbara). Tick infestation remains unreported for 5 of the 26 Carnivora species native in Brazil: Oncifelis geoffroyi (Geoffroy's cat), Atelocynus microtis (short-eared dog), Pteronura brasiliensis (giant otter), Mustela africana (Amazon weasel), and Bassaricyon gabbii (olingo). Our field data comprise 16 tick species represented by the genera Amblyomma (12 species), Ixodes (1

  16. New feather mites of the subfamily Pterodectinae (Acari: Astigmata: Proctophyllodidae) from passerines (Aves: Passeriformes) in Mato Grosso do Sul, Brazil

    Czech Academy of Sciences Publication Activity Database

    Mironov, S. V.; Literák, I.; Čapek, Miroslav

    2008-01-01

    Roč. 1947, - (2008), s. 1-38 ISSN 1175-5326 Institutional research plan: CEZ:AV0Z60930519 Keywords : Acari * Astigmata * feather mites * systematics * Brazil * Proctophyllodidae * Aves * Passeriformes Subject RIV: EG - Zoology Impact factor: 0.740, year: 2008 http://www.mapress.com/zootaxa/2008/f/zt01947p038.pdf

  17. Response of forest soil Acari to prescribed fire following stand structure manipulation in the southern Cascade Range.Can

    Science.gov (United States)

    Michael A. Camann; Nancy E. Gillette; Karen L. Lamoncha; Sylvia R. Mori

    2008-01-01

    We studied responses of Acari, especially oribatid mites, to prescribed low-intensity fire in an east side pine site in the southern Cascade Range in California. We compared oribatid population and assemblage responses to prescribed fire in stands that had been selectively logged to enhance old growth characteristics, in logged stands to minimize old growth...

  18. Effect of coconut palm proximities and Musa spp. germplasm resistance to colonization by Raoiella indica (Acari: Tenuipalpidae)

    Science.gov (United States)

    Although coconut (Cocos nucifera L.) is the predominant host for Raoiella indica Hirst (Acari: Tenuipalpidae), false spider mite infestations do occur on bananas and plantains (Musa spp. Colla). Since its introduction, the banana and plantain industries have been negatively impacted to different deg...

  19. The Genus Ixodes (Acari: Ixodidae) in Mexico: Adult Identification Keys, Diagnoses, Hosts, and Distribution

    Science.gov (United States)

    2010-01-01

    Cricetidae and Hominidae ( Homo sapiens Linnaeus, 1758) (Mammalia) (Hoffmann, 1969). Note. The tick from Valle de Bravo, Estado de México, was...distribution El género Ixodes (Acari: Ixodidae) en México: claves de identifi cación para adultos, diagnosis, huéspedes y distribución Carmen Guzmán...Incorporación de Profesores de Carrera en Facultades y Escuelas para el Fortalecimiento de la Investigación (PROFIP). Tila María Pérez, Curator of CNAC

  20. Mites (Acari Associated with the Desert Seed Harvester Ant, Messor pergandei (Mayr

    Directory of Open Access Journals (Sweden)

    Kaitlin A. Uppstrom

    2011-01-01

    Full Text Available Mites (Acari associated with the seed harvester ant Messor pergandei were investigated in the Sonoran desert of Arizona. At least seven representatives of the mite genera Armacarus, Lemanniella, Petalomium, Forcellinia, Histiostoma, Unguidispus, and Cosmoglyphus are phoretically associated with M. pergandei. Most of these morphospecies show preference for specific phoretic attachment sites and primarily use female alates rather than male alates for dispersal. Five mite morphospecies were found in low numbers inhabiting the chaff piles: Tydeidae sp., Procaeculus sp., Anystidae sp., Bakerdania sp., and Tetranychidae sp. The phoretic Petalomium sp. was observed consuming fungus growing on a dead queen, but the roles of the other mite species remain mostly unresolved.

  1. The impact of cucumber nitrogen nutrition on life history traits of Tetranychus urticae (Koch) (Acari: Tetranychidae)

    OpenAIRE

    Motahari , M.; Kheradmand , K.; Roustaee , A.M.; Talebi , A.A.

    2014-01-01

    International audience; The nutritional quality of the host plant is one of the most important factors of growth and reproduction for crop pests. In order to investigate the impact of nitrogen on the biology and demography of Tetranychus urticae Koch (Acari: Tetranychidae), cucumber plants were nourished by four levels of nitrogen: N1 (10 meq /L NO3-), N2 (12 meq/L NO3-), N3 (15 meq/L NO3-) and N4 (20 meq /L NO3-). The experiments were performed under laboratory conditions at 25+/-1 °C, 60+/-...

  2. The residual and direct effects of reduced-risk and conventional miticides on twospotted spider mites, Tetranychus urticae (Acari: Tetranychidae) and predatory mites (Acari: Phytoseiidae)

    International Nuclear Information System (INIS)

    Liburd, O.E.; White, J.C.; Rhodes, E.M.; Browdy, A.A.

    2007-01-01

    The residual effects of several reduced-risk and conventional miticides were evaluated in strawberries (Fragaria × ananassa Duchesne) on the twospotted spider mite (TSSM), Tetranychus urticae Koch (Acari: Tetranychidae) and on 2 predatory mites, Neoseiulus californicus McGregor and Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae). Experiments were conducted in the laboratory and greenhouse. The greenhouse experiments also tested the direct effects of the miticides on TSSM. The efficacy of conventional and reduced-risk miticides was evaluated on strawberry leaf discs and on whole plants for control of TSSM. Furthermore, the residual effects of these miticides were evaluated on whole strawberry plants against selective predatory mites. For TSSM, 5 treatments were evaluated: a conventional miticide; fenbutatin-oxide (Vendex[reg]) and 3 reduced-risk miticides; binfenazate (Acramite 50WP[reg]), activated garlic extract (Repel[reg]), sesame seed and castor oil (Wipeout[reg]), and a water-treated control. For predatory mites, the residual effects of only Acramite[reg] and Vendex[reg] were evaluated. Acramite[reg] was the most effective acaricide in reducing TSSM populations in both the laboratory and greenhouse experiments. Vendex[reg] and Wipeout[reg] were also effective in the laboratory, but did not cause significant reduction of TSSM in the greenhouse. Repel[reg] was the least effective of the 4 pesticides evaluated. Neither Acramite[reg] nor Vendex[reg] had a significant effect on either predatory mite species. However, there appeared to be more predatory mites on the Vendex[reg]-treated plants than on the Acramite[reg]-treated plants. There were significantly more predatory mites of both species on the cue plants, which were inoculated with TSSM versus the non-cue plants, which were not inoculated. (author) [es

  3. Reproductive parameters of Phytoseiulus macropilis (Banks fed with Tetranychus urticae Koch (Acari: Phytoseiidae, Tetranychidae in laboratory

    Directory of Open Access Journals (Sweden)

    G. C. Souza-Pimentel

    Full Text Available Abstract Predatory mites that belong to the Phytoseiidae family are one of the main natural enemies of phytophagous mites, thus allowing for their use as a biological control. Phytoseiulus macropilis (Banks, 1904 (Acari: Phytoseiidae is among the main species of predatory mites used for this purpose. Tetranychus urticae Koch, 1836 (Acari: Tetranychidae is considered to be one of the most important species of mite pests and has been described as attacking over 1,100 species of plants in 140 families with economic value. The objective of the present study was to investigate, in the laboratory, the reproductive parameters of the predatory mite P. macropilis when fed T. urticae. Experiments were conducted under laboratory conditions at 25 ± 2 °C of temperature, 70 ± 10% RH and 14 hours of photophase. In addition, biological aspects were evaluated and a fertility life table was established. The results of these experiments demonstrated that the longevity of adult female was 27.5 days and adult male was 29.0 days. The population was estimated to increase approximately 27 times (Ro in mean generation time (T, which was 17.7 days. Lastly, the mite population grew 1.2 times/day (λ and doubled every 3.7 days (TD.

  4. Plant architecture and prey distribution influence foraging behavior of the predatory mite Phytoseiulus persimilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Gontijo, Lessando M; Nechols, James R; Margolies, David C; Cloyd, Raymond A

    2012-01-01

    The arrangement, number, and size of plant parts may influence predator foraging behavior, either directly, by altering the rate or pattern of predator movement, or, indirectly, by affecting the distribution and abundance of prey. We report on the effects of both plant architecture and prey distribution on foraging by the predatory mite, Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae), on cucumber (Cucumis sativus L.). Plants differed in leaf number (2- or 6-leafed), and there were associated differences in leaf size, plant height, and relative proportions of plant parts; but all had the same total surface area. The prey, the twospotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae), were distributed either on the basal leaf or on all leaves. The effect of plant architecture on predator foraging behavior varied depending on prey distribution. The dimensions of individual plant parts affected time allocated to moving and feeding, but they did not appear to influence the frequency with which predators moved among different plant parts. Overall, P. persimilis moved less, and fed upon prey longer, on 6-leafed plants with prey on all leaves than on plants representing other treatment combinations. Our findings suggest that both plant architecture and pattern of prey distribution should be considered, along with other factors such as herbivore-induced plant volatiles, in augmentative biological control programs.

  5. Development, oviposition, and mortality of Neoseiulus fallacis (Acari: Phytoseiidae) in response to reduced-risk insecticides.

    Science.gov (United States)

    Villanueva, Raul T; Walgenbach, James F

    2005-12-01

    Eight reduced-risk insecticides (acetamiprid, thiamethoxam, imidacloprid, thiacloprid, methoxyfenozide, pyriproxyfen, indoxacarb, and spinosad) and three conventional insecticides (azinphosmethyl, fenpropathrin, and esfenvalerate) were tested against Neoseiulus fallacis (Garman) (Acari: Phytoseiidae), the most abundant predacious mite in North Carolina apple (Malus spp.) orchards. To assess the effect of insecticides on development and mortality of N. fallacis immatures, 12-h-old eggs were individually placed on bean leaf disks previously dipped in insecticide solutions. Tetranychus urticae Koch (Acari: Tetranychidae) females were added as a food source. None of the reduced-risk insecticides significantly affected immature N. fallacis compared with the control; however, the pyrethroids esfenvalerate and fenpropathrin were highly toxic to immatures. To evaluate the effect of insecticides on mortality and oviposition of adult N. fallacis, 7- to 8-d-old females were confined on insecticide-treated bean leaves with Malephora crocea (Aizoaceae) pollen added as a food source. Spinosad resulted in the highest mortality, whereas azinphosmethyl, acetamiprid, fenpropathrin, and imidacloprid were moderately toxic, and mortality from esfenvalerate, indoxacarb, thiacloprid, methoxyfenozide, pyriproxyfen, and thiamethoxam did not differ significantly from the control. Oviposition was affected in a similar manner, with the exception of acetamiprid that did not affect oviposition, and thiamethoxam that reduced oviposition.

  6. Phytoseiid mites of the Canary Islands (Acari, Phytoseiidae. II. Tenerife and La Gomera Islands

    Directory of Open Access Journals (Sweden)

    Ferragut, F.

    2007-12-01

    Full Text Available Phytoseiid mites (Acari, Phytoseiidae inhabiting plants in natural ecosystems from Tenerife and La Gomera islands (Canary Islands have been studied. Surveys were conducted from 1997 to 2002. Eleven species were collected, one of them being reported for the first time from the Canary Islands and six of them reported for the first time from Tenerife and La Gomera islands. Euseius machadoi n. sp. collected from woody plants in the Canarian laurisilva is proposed as a new species.

    En muestreos realizados desde 1997 hasta 2002 se ha estudiado la fauna de ácaros fitoseidos (Acari, Phytoseiidae asociada a plantas de ecosistemas naturales de las islas de Tenerife y La Gomera (Islas Canarias. Se han recolectado un total de 11 especies, siendo una de ellas citada por vez primera en las islas Canarias y seis de ellas citadas por primera vez en las islas de Tenerife y La Gomera. Euseius machadoi n. sp., recolectado en plantas leñosas de la laurisilva canaria, se propone como una nueva especie.

  7. Infestação de Amblyomma rotundatum (Koch (Acari, Ixodidae em sapos Bufo ictericus (Spix (Amphibia, Bufonidae: novo registro de hospedeiro Infestation of Amblyomma rotundatum (Koch (Acari, Ixodidae ticks on Bufo ictericus (Spix (Amphibia, Bufonidae: new host record

    Directory of Open Access Journals (Sweden)

    Germano Woehl Jr.

    2002-06-01

    Full Text Available Bufo ictericus Spix, 1824 toad population (N = 125 foraging in lighted areas in the Corupá Municipality, Santa Catarina State, was surveyed to evaluate the prevalence (percentage of infestation and the mean infestation intensity of Amblyomma rotundatum Koch, 1844 (Acari, Ixodidae ticks. The prevalence was of 19.2% and the mean infestation intensity was 7.4 ticks per infested toad. For the first time B. ictericus as host of A. rotundatum is reported.

  8. Community structure variability of Uropodina mites (Acari: Mesostigmata) in nests of the common mole, Talpa europaea, in Central Europe

    OpenAIRE

    Napiera?a, Agnieszka; M?dra, Anna; Leszczy?ska-Deja, Kornelia; Gwiazdowicz, Dariusz J.; Go?dyn, Bart?omiej; B?oszyk, Jerzy

    2016-01-01

    Underground nests of Talpa europaea, known as the common mole, are very specific microhabitats, which are also quite often inhabited by various groups of arthropods. Mites from the suborder Uropodina (Acari: Mesostigmata) are only one of them. One could expect that mole nests that are closely located are inhabited by communities of arthropods with similar species composition and structure. However, results of empirical studies clearly show that even nests which are close to each other can be ...

  9. First record of larvae of the water mite Hydrachna processifera Piersig, 1895 from Turkey (Acari, Hydrachnidia, Hydrachnidae

    Directory of Open Access Journals (Sweden)

    Medeni Aykut

    2018-02-01

    Full Text Available Larvae of water mite Hydrachna processifera Piersig, 1895 (Acari, Hydrachnidiae were reported on diving beetles Dytiscus marginalis Linnaeus, 1758 (Coleoptera, Dytiscidae from Turkey. The redescription of the larva was made. Earlier, the larva H. processifera was described as H. inermis, but it was subsequently synonymized with H. processifera. The larva of H. processifera is a new record for the Turkish fauna. All larvae of H. processifera were found on the mesosternum of the one specimens (prevalence = 16.7%.

  10. Laboratory evaluation of the effect of Beauveria bassiana on the predatory mite Phytoseiulus persimilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Ullah, Mohammad Shaef; Lim, Un Taek

    2017-09-01

    Tetranychus urticae Koch (Acari: Tetranychidae), a major pest of many agricultural crops, is mainly controlled with chemical acaricides. However, predatory mites and entomopathogens have been proposed as alternative control agents. In this study, the effect of the BotaniGard ® GHA strain of Beauveria bassiana on the survival, longevity, fecundity, and egg hatch rate of the predatory mite Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae) were studied under laboratory conditions. When B. bassiana was applied directly to P. persimilis eggs at a concentration of 1×10 8 conidia/ml, corrected hatchability was less than 5%, and the corrected mortality of nymphs and adults was not significantly different from control 10days after treatment. Phytoseiulus persimilis nymphs that hatched from treated eggs showed no significant change in their development time, adult female longevity, hatch rate, survival rates over time, or offspring sex ratio. However, significant negative effects on fecundity and life table parameters (net reproductive rate, intrinsic rate of natural increase, mean generation time, finite rate of increase, and doubling time) were found when B. bassiana was applied to the adult stage. Spraying B. bassiana at 1×10 8 conidia/ml on newly emerged adults of P. persimilis caused 44% reduction in the oviposition period, 26% in adult longevity, and 63% in fecundity. Due to these negative effects, B. bassiana should be used with careful adjustment of application timing (first spray B. bassiana and then release P. persimilis) to supplement biological mite control systems using P. persimilis. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. First record of the genus .i.Bloszykiella./i. in Kenya with the description of .i.Bloszykiella tertia./i. sp n. (Acari: Uropodidae) from a .i.Pinus radiata./i. D. Don plantation

    Czech Academy of Sciences Publication Activity Database

    Kontschán, J.; Starý, Josef

    2015-01-01

    Roč. 56, č. 3 (2015), s. 629-635 ISSN 1681-5556 Institutional support: RVO:60077344 Keywords : Acari * new species * Pinus radiata plantation * Kenya Subject RIV: EG - Zoology Impact factor: 0.708, year: 2015

  12. New records of water mites of the genus Atractides Koch, 1837 (Acari: Hydrachnidia, Hygrobatidae) from Thailand, Malaysia and Sulawesi (Indonesia), with the description of four new species

    NARCIS (Netherlands)

    Pesic, V.; Smit, H.

    2009-01-01

    New records of water mites of the genus Atractides Koch, 1837 (Acari: Hydrachnidia, Hygrobatidae) from Thailand, Malaysia and Sulawesi are presented. Four species new to science, Atractides siamis, A. malayensis, A. tahanensis and A. sulawesiensis, are described; a first description of the male is

  13. The Genus Ixodes (Acari: Ixodidae) in Mexico: Adult Identification Keys, Diagnoses, Hosts, and Distribution (El genero Ixodes (Acari: Ixodidae) en Mexico: claves de identificacion para adultos, diagnosis, huespedes y distribucion)

    Science.gov (United States)

    2010-01-01

    also from Estado de México (Hoffmann, 1969). Hosts in Mexico. Cricetidae and Hominidae ( Homo sapiens Linnaeus, 1758) (Mammalia) (Hoffmann, 1969...distribution El género Ixodes (Acari: Ixodidae) en México: claves de identifi cación para adultos, diagnosis, huéspedes y distribución Carmen Guzmán...postdoctoral scholarship under the Programa de Formación e Incorporación de Profesores de Carrera en Facultades y Escuelas para el Fortalecimiento de la

  14. New species and new records of mites of the genus Stigmaeus(Acari: Prostigmata: Stigmaeidae) from Crimea.

    Science.gov (United States)

    Khaustov, Alexander A

    2014-05-06

    Three new species of the genus Stigmaeus Koch, 1836 (Acari: Stigmaeidae) are described from various habitats in Crimea: Stigmaeus kuznetsovi sp. nov. from nests of Microtus socialis (Rodentia: Cricetidae); S. mitrofanovi sp. nov. from galleries of Pityogenes bistridentatus (Coleoptera: Curculionidae) under the bark of Pinus pallasiana, and S. silvestris sp. nov. from rotten log of Pinus pallasiana. Stigmaeus corticeus Kuznetsov and Wainstein, 1977 and S. maraghehiensis Bagheri and Ueckermann, 2012 are recorded for the first time in Crimea. A key to species of the genus Stigmaeus of Crimea is provided.

  15. Infection of the Gulf Coast Tick, Amblyomma Maculatum (Acari: Ixodidae), with Rickettsia Parkeri: First Report from the State of Delaware

    Science.gov (United States)

    2013-03-31

    0279276E-D761-4A27-BFF7-7329E05E0F66 Infection of the Gulf Coast tick, Amblyomma maculatum (Acari: Ixodidae), with Rickettsia parkeri: first report from...Spring, MD 20910-1230, U.S.A. Abstract The molecular detection of Rickettsia parkeri in a Gulf Coast tick, Amblyomma maculatum, collected in Delaware...near Smyrna, Delaware. All specimens were tested for the presence of Rickettsia with a genus-specific quantitative real-time polymerase chain

  16. Primeiro registro de Oligonychus yothersi (McGregor (Acari: Tetranychidae em Eucalyptus grandis Hill ex Maiden no Brasil First record of Oligonychus yothersi (McGregor (Acari: Tetranychidae on Eucalyptus grandis Hill ex Maiden in Brazil

    Directory of Open Access Journals (Sweden)

    Fabrício Fagundes Pereira

    2005-08-01

    Full Text Available Relata-se a infestação de um ácaro-vermelho em mudas clonais de Eucalyptus grandis Hill ex Maiden, mantidas em casa de vegetação no município de Martinho Campos, Minas Gerais. O ácaro foi observado na parte superior das folhas que exibiam sinais de sucção de seiva e bronzeamento. Essas injúrias causaram desenvolvimento anormal e morte de plantas. O ácaro foi identificado como Oligonychus yothersi (McGregor (Acari: Tetranychidae, e isso representa o primeiro registro dessa espécie em mudas clonais de E. grandis no Brasil.An infestation of the red spider mite was reported in clone seedlings of Eucalyptus grandis Hill ex Maiden under greenhouse conditions, in the municipality of Martinho Campos, Minas Gerais State. The spider mite was found on the leaf upper faces with signs of sap suction and bronzing. Such injuries caused abnormal development and plant death. The spider mite was identified as Oligonychus yothersi (McGregor (Acari: Tetranychidae. This is the first record of O. yothersi on E. grandis seedlings in Brazil.

  17. Acaricidal and repellent activities of essential oil of Eucalyptus globulus against Dermanyssus gallinae (Acari: Mesostigmata

    Directory of Open Access Journals (Sweden)

    Dehghani-Samani Amir

    2015-07-01

    Full Text Available Introduction: By considering an increase in drug resistance against red mites, finding the nonchemical herbal acaricide against Dermanyssus gallinae (De Geer (Acari: Mesostigmata is necessary to kill them and to reduce the chemical resistance against chemical acaricides in this specie. Dermanyssus gallinae is a potential vector of the causal agent of several viral diseases such as Equine encephalitis and St. Louis encephalitis. It can be a vector of bacteria such as Salmonella spp., Mycobacterium spp. and Erysipelothrix rhusiopathiae. It is also known to cause itching dermatosis in humans. In this study acaricidal and repellent activities of essential oil of Eucalyptus globulus against Dermanyssus gallinae were studied. Methods: After extracting the essential oil, different concentrations of the plant extract were prepared. Then, acaricidal effect of different concentrations was tested on poultry red mite, Dermanyssus gallinae, by dropping 3-4 drops of essential oil on mites. Repellent activity of essential oil was tested by Y-tube olfactometer bioassay. After the test, total number of killed and repellent mites reported. Results: Concentration of 1:2 or 50% had more acaricidal effect on mites. Also essential oil of Eucalyptus globulus had repellent activity against red mites. Conclusion: This study showed that essential oil of Eucalyptus globulus had acaricidal and repellent activities against red mites. Hence it might be used as a herbal acaricide against it to kill and to reduce the chemical resistance in this specie.

  18. Ticks (Acari: Ixodidae of livestock and their seasonal activities, northwest of Iran

    Directory of Open Access Journals (Sweden)

    Zahra Ramezani

    2014-09-01

    Full Text Available Objective: To identify the ticks (Acari: Ixodidae of livestock and their seasonal activities, in northwest of Iran, including the combination of two of the geographical regions of Iran (Caspian and mountain plateau where the majority of the domestic ruminants in Iran exist. Methods: Fifteen villages of Meshkin-Shahr County were selected randomly from different areas of the county. The animal dwellings were visited and the whole body of sheep, cows, goats and dogs were examined for their probable infestation. Samples were identified at the level of species according to the standard morphological key. Results: In this study 1 208 specimen were collected and totally nine species (Dermacentor marginatus, Dermacentor niveus, Haemaphysalis erinacei, Haemaphysalis punctata, Hyalomma anatolicum, Hyalomma asiaticum, Hyalomma marginatum, Rhipicephalus bursa and Rhipicephalus sanguineus were identified in this study. Also 569 host including 40 cows, 450 sheep, 70 goats and 9 dogs were examined for infestation and among them 255 were infested which showed a 44% of infestation among examined livestock. The infestation rate among sheep (46% was higher than other hosts. The infestation rates among the rest of hosts were as: cows (40%, goat (37% and dogs (33%. Conclusions: The results of this study and other studies of the region showed the probability of the establishment and development of the burden of several tick-borne diseases.

  19. Study of Acari and Collembola Populations in Four Cultivation Systems in Dourados - MS

    Directory of Open Access Journals (Sweden)

    Rosilda Mara Mussury

    2002-09-01

    Full Text Available The impact four cultivation systems on the soil fauna was studied, using Oribatida and Gamasida acarids as bioindicators and collembolan. The research was carried out in experimental fields, located in EMBRAPA - CPAO in Dourados, Centerwest of Brazil from July 1997 to December 1999. The constant pasture system presented smaller impact on the soil fauna followed by agricultural cattle rotation and a direct plantation system. In the conventional plantation series, the populational density of the mesofauna organisms was low, especially collembolan families.O impacto de quatro sistemas de cultivo sobre a fauna de solo foram estudados, utilizando-se como bioindicadores os acari Oribatida e Gamasida e os Collembola. A pesquisa foi conduzida em campos experimentais, localizados na EMBRAPA - CPAO no município de Dourados, MS, no período de julho de 1997 à dezembro de 1999. O sistema de pastagem contínua apresentou menor impacto sobre a fauna de solo seguido da rotação agricultura pecuária e do sistema de plantio direto. Nas sucessões do plantio convencional, a densidade populacional dos organismos da mesofauna foi baixa, em especial as famílias de colembolos.

  20. Rickettsia parkeri and "Candidatus Rickettsia andeanae" in Questing Amblyomma maculatum (Acari: Ixodidae) From Mississippi.

    Science.gov (United States)

    Lee, J K; Moraru, G M; Stokes, J V; Wills, R W; Mitchell, E; Unz, E; Moore-Henderson, B; Harper, A B; Varela-Stokes, A S

    2017-03-01

    Amblyomma maculatum Koch (Acari: Ixodidae), the primary vector for Rickettsia parkeri, may also be infected with a rickettsia of unknown pathogenicity, "Candidatus Rickettsia andeanae." Infection rates with these rickettsiae vary geographically, and coinfected ticks have been reported. In this study, infection rates of R. parkeri and "Ca. R. andeanae" were evaluated, and rickettsial DNA levels quantified, in 335 questing adult A. maculatum collected in 2013 (n = 95), 2014 (n = 139), and 2015 (n = 101) from Oktibbeha County, MS. Overall infection rates of R. parkeri and "Ca. R. andeanae" were 28.7% and 9.3%, respectively, with three additional A. maculatum (0.9%) coinfected. While R. parkeri-infected ticks were detected all three years (34.7% in 2013; 13.7% in 2014; 43.6% in 2015), "Ca. R. andeanae" was not detected in 2013, and was detected at rates of 10.8% in 2014, and 15.8% in 2015. Interestingly, rickettsial DNA levels in singly-infected ticks were significantly lower in "Ca. R. andeanae"-infected ticks compared to R. parkeri-infected ticks (P Rickettsia species in A. maculatum at the population level. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  1. Biology, ecology and control of the Penthaleus species complex (Acari: Penthaleidae).

    Science.gov (United States)

    Umina, Paul A; Hoffmann, Ary A; Weeks, Andrew R

    2004-01-01

    Blue oat mites, Penthaleus spp. (Acari: Penthaleidae), are major agricultural pests in southern Australia and other parts of the world, attacking various pasture, vegetable and crop plants. Management of these mites has been complicated by the recent discovery of three cryptic pest species of Penthaleus, whereas prior research had assumed a single species. The taxonomy, population genetics, ecology, biology and control of the Penthaleus spp. complex are reviewed. Adult Penthaleus have a dark blue-black body approximately 1 mm in length, and eight red-orange legs. Within Australia, they are winter pests completing two or three generations a season, depending on conditions. The summer is passed as diapausing eggs, when long-distance dispersal is thought to occur. The Penthaleus spp. reproduce by thelytokous parthenogenesis, with populations comprising clones that differ ecologically. The three pest Penthaleus spp. differ markedly in their distributions, plant hosts, timing of diapause egg production and response to pesticides, highlighting the need to develop control strategies that consider each species separately. Chemicals are the main weapons used in current control programs, however research continues into alternative more sustainable management options. Host plant resistance, crop rotations, conservation of natural enemies, and improved timing of pesticide application would improve the management of these pests. The most cost-effective and environmentally acceptable means of control will result from the integration of these practices combined with the development of a simple field-based kit to distinguish the different mite species.

  2. Evidence for host plant preference by Iphiseiodes quadripilis (Acari: Phytoseiidae) on Citrus.

    Science.gov (United States)

    Villanueva, Raul T; Childers, Carl C

    2006-01-01

    In this study, we present field and laboratory evidence on the preference of Iphiseiodes quadripilis (Banks) for grapefruit (Citrus paradisi Macfadyen) leaves compared with sweet orange (Citrus sinensis (L.) Osbeck) leaves. This preference was confirmed in four orchards whether leaf samples were taken from either border trees of contiguous grapefruit or sweet orange or interior row trees with both citrus species in adjacent rows. Iphiseiodes quadripilis was most abundant in grapefruit trees in spite of the greater abundance of the Texas citrus mite, Eutetranychus banksi (McGregor) (Acari: Tetranychidae) in sweet orange trees. Similar preference responses were observed in laboratory tests using a Y-tube olfactometer whether I. quadripilis were collected from sweet orange or grapefruit. Iphiseiodes quadripilis collected from grapefruit trees showed significant preference for grapefruit over sweet orange leaves in contact choice tests using an arena of alternating leaf strips (12 mm long x 2 mm wide) of sweet orange and grapefruit. However, I. quadripilis collected from sweet orange trees did not show preference for either grapefruit or sweet orange leaves. Based on these results, grapefruit leaves foster some unknown factor or factors that retain I. quadripilis in greater numbers compared with sweet orange leaves.

  3. Cuticle expansion during feeding in the tick Amblyomma hebraeum (Acari: Ixodidae): The role of hydrostatic pressure.

    Science.gov (United States)

    Kaufman, W Reuben; Kaufman, S; Flynn, Peter C

    2016-05-01

    Female Amblyomma hebraeum ticks (Acari: Ixodidae) increase their weight ∼10-fold during a 'slow phase of engorgement' (7-9 days), and a further 10-fold during the 'rapid phase' (12-24h). During the rapid phase, the cuticle thins by half, with a plastic (permanent) deformation of greater than 40% in two orthogonal directions. A stress of 2.5 MPa or higher is required to achieve this degree of deformation (Flynn and Kaufman, 2015). Using a dimensional analysis of the tick body and applying the Laplace equation, we calculated that the tick must achieve high internal hydrostatic pressures in order to engorge fully: greater than 55 kPa at a fed:unfed mass ratio of ∼20:1, when cuticle thinning commences (Flynn and Kaufman, 2011). In this study we used a telemetric pressure transducer system to measure the internal hydrostatic pressure of ticks during feeding. Sustained periods of irregular high frequency (>20 Hz) pulsatile bursts of high pressure (>55 kPa) were observed in two ticks: they had been cannulated just prior to the rapid phase of engorgement, and given access to a host rabbit for completion of the feeding cycle. The pattern of periods of high pressure generation varied over the feeding cycle and between the two specimens. We believe that these pressures exceed those reported so far for any other animal. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. Essential oils of aromatic Egyptian plants repel nymphs of the tick Ixodes ricinus (Acari: Ixodidae).

    Science.gov (United States)

    El-Seedi, Hesham R; Azeem, Muhammad; Khalil, Nasr S; Sakr, Hanem H; Khalifa, Shaden A M; Awang, Khalijah; Saeed, Aamer; Farag, Mohamed A; AlAjmi, Mohamed F; Pålsson, Katinka; Borg-Karlson, Anna-Karin

    2017-09-01

    Due to the role of Ixodes ricinus (L.) (Acari: Ixodidae) in the transmission of many serious pathogens, personal protection against bites of this tick is essential. In the present study the essential oils from 11 aromatic Egyptian plants were isolated and their repellent activity against I. ricinus nymphs was evaluated Three oils (i.e. Conyza dioscoridis L., Artemisia herba-alba Asso and Calendula officinalis L.) elicited high repellent activity in vitro of 94, 84.2 and 82%, respectively. The most active essential oil (C. dioscoridis) was applied in the field at a concentration of 6.5 µg/cm 2 and elicited a significant repellent activity against I. ricinus nymphs by 61.1%. The most repellent plants C. dioscoridis, C. officinalis and A. herba-alba yielded essential oils by 0.17, 0.11 and 0.14%, respectively. These oils were further investigated using gas chromatography-mass spectrometry analysis. α-Cadinol (10.7%) and hexadecanoic acid (10.5%) were the major components of C. dioscoridis whereas in C. officinalis, α-cadinol (21.2%) and carvone (18.2%) were major components. Artemisia herba-alba contained piperitone (26.5%), ethyl cinnamate (9.5%), camphor (7.7%) and hexadecanoic acid (6.9%). Essential oils of these three plants have a potential to be used for personal protection against tick bites.

  5. Aggregation in the tick Ixodes ricinus (Acari: Ixodidae): use and reuse of questing vantage points.

    Science.gov (United States)

    Healy, John A E; Bourke, Patrick

    2008-03-01

    Ongoing work in oak woods in Killarney National Park in southwestern Ireland is focusing on the factors influencing the fine-scale aggregated distribution of Ixodes ricinus L. (Acari: Ixodidae) on the ground. The extent of reuse of stems of vegetation as questing points by adult ticks was determined by paint-marking stems on which ticks were found, counting and removing these ticks, and subsequently reexamining the same stems for ticks on two further occasions. Overall, an estimated 2,967 stems in 123 separate rush plants (Juncus effusus L.) were examined. Statistical analysis of the data demonstrated a highly significant reoccupancy by ticks of stems previously and recently used. Furthermore, it is shown that the extent of stem reuse by ticks is significantly and positively correlated with the numbers of ticks originally observed on those stems. Although other factors may be involved in generating clumping of ticks, the results are compatible with the proposition that aggregation of I. ricinus on the ground is pheromone-mediated. The findings are discussed in relation to what is known about the powers of lateral movement of I. ricinus on the ground and the possible implications for the performance of tick traps.

  6. Morphometric variations of laelapine mite (Acari: Mesostigmata populations infesting small mammals (Mammalia in Brazil

    Directory of Open Access Journals (Sweden)

    F. Martins-Hatano

    Full Text Available The goal of this study was to evaluate the morphometric variation of laelapine populations (Acari, Mesostigmata associated with neotropical oryzomyine rodents at different geographic localities in Brazil. Three nominal mite species were selected for study, all infesting the pelage of small mammals at different localities in Rio de Janeiro, Espírito Santo, Bahia, and the Federal District, Brazil. To analyse morphometric characteristics, thirty-seven morphological characters distributed across the whole body of each specimen were measured. We use the Analysis of Principal Components, extracting the three first axes and projecting each mite in these axes. Major species level changes in the taxonomy of the host mammals allows an independent examination of morphometric variation of mites infesting a set of distinctly different host species at different geographic localities. Gigantolaelaps vitzthumi and Laelaps differens are associated with oryzomyine rodents of the genus Cerradomys, and consistently showed a tendency to cluster by host phylogeny. Laelaps manguinhosi associated with Nectomys rattus in central Brazil is morphometrically distinct from mites infesting N. squamipes in the coastal restingas of Rio de Janeiro and Espírito Santo. The results obtained here indicate that laelapine mite populations can vary among geographic areas and among phylogenetically related host species. Clearly, the study of these mites at the population level can be an important tool for clarifying the taxonomy of both mites and hosts.

  7. Morphometric variations of laelapine mite (Acari: Mesostigmata) populations infesting small mammals (Mammalia) in Brazil.

    Science.gov (United States)

    Martins-Hatano, F; Gettinger, D; Manhães, M L; Bergallo, H G

    2012-08-01

    The goal of this study was to evaluate the morphometric variation of laelapine populations (Acari, Mesostigmata) associated with neotropical oryzomyine rodents at different geographic localities in Brazil. Three nominal mite species were selected for study, all infesting the pelage of small mammals at different localities in Rio de Janeiro, Espírito Santo, Bahia, and the Federal District, Brazil. To analyse morphometric characteristics, thirty-seven morphological characters distributed across the whole body of each specimen were measured. We use the Analysis of Principal Components, extracting the three first axes and projecting each mite in these axes. Major species level changes in the taxonomy of the host mammals allows an independent examination of morphometric variation of mites infesting a set of distinctly different host species at different geographic localities. Gigantolaelaps vitzthumi and Laelaps differens are associated with oryzomyine rodents of the genus Cerradomys, and consistently showed a tendency to cluster by host phylogeny. Laelaps manguinhosi associated with Nectomys rattus in central Brazil is morphometrically distinct from mites infesting N. squamipes in the coastal restingas of Rio de Janeiro and Espírito Santo. The results obtained here indicate that laelapine mite populations can vary among geographic areas and among phylogenetically related host species. Clearly, the study of these mites at the population level can be an important tool for clarifying the taxonomy of both mites and hosts.

  8. Comparison of thermal activity thresholds of the spider mite predators Phytoseiulus macropilis and Phytoseiulus persimilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Coombs, Megan R; Bale, Jeffrey S

    2013-04-01

    The lower and upper thermal activity thresholds of the predatory mite Phytoseiulus macropilis Banks (Acari: Phytoseiidae) were compared with those of its prey Tetranychus urticae Koch (Acari: Tetranychidae) and one of the alternative commercially available control agents for T. urticae, Phytoseiulus persimilis Athias-Henriot. Adult female P. macropilis retained ambulatory function (CTmin) and movement of appendages (chill coma) at significantly lower temperatures (8.2 and 0.4 °C, respectively) than that of P. persimilis (11.1 and 3.3 °C) and T. urticae (10.6 and 10.3 °C). As the temperature was raised, P. macropilis ceased walking (CTmax) and entered heat coma (42.7 and 43.6 °C), beyond the upper locomotory limits of P. persimilis (40.0 and 41.1 °C), but before T. urticae (47.3 and 48.7 °C). Walking speeds were investigated and P. persimilis was found to have significantly faster ambulation than P. macropilis and T. urticae across a range of temperatures. The lower thermal activity threshold data indicate that P. macropilis will make an effective biological control agent in temperate climates.

  9. Ricoseius loxocheles (Acari: Phytoseiidae) is not a predator of false spider mite on coffee crops: What does it eat?

    Science.gov (United States)

    Vacacela Ajila, Henry E; Ferreira, João A M; Colares, Felipe; Oliveira, Cleber M; Bernardo, Ana Maria G; Venzon, Madelaine; Pallini, Angelo

    2018-01-01

    Ricoseius loxocheles (De Leon) (Acari: Phytoseiidae) is often found in coffee crops and is known to feed on coffee leaf rust, Hemileia vastatrix Berkeley and Broome (Uredinales). As the occurrence of coffee leaf rust is limited primarily to the rainy season, the mite may use other food sources to survive during the periods of low pathogen prevalence. It is well known that phytoseiid mites can survive on a variety of food sources, such as herbivorous mites, fungi and pollen. We evaluated the ability of R. loxocheles to survive and reproduce on a diet of Brevipalpus phoenicis Geijskes (Acari: Tenuipalpidae), cattail pollen (Typha spp.), clover rust (Puccinia oxalidis), bee pollen (Santa Bárbara ® dehydrated pollen, Santa Bárbara, MG, Brazil) and coffee leaf rust. Ricoseius loxocheles did not survive or reproduce on any B. phoenicis stages tested (egg, larva, adult). The survival and oviposition of R. loxocheles were directly affected by the presence of coffee rust urediniospores, but not by the presence of the prey. Survival and oviposition of the phytoseiid were similar when fed cattail pollen, clover rust and coffee leaf rust but was lower when fed bee pollen. Our results show that R. loxocheles is not a predator of B. phoenicis but it is able to utilize other resources besides coffee leaf rust.

  10. Caracterización de la actividad alergénica y enzimática de extractos somáticos producidos a partir de cultivos in vitro del ácaro Dermatophagoide farinae

    Directory of Open Access Journals (Sweden)

    Dary Luz Mendoza Meza

    2011-01-01

    Full Text Available Introduccion: El Dermatophagoides fainae (DF es un acaro cosmopolita presente en el polvo intradomiciliario. Este acaro es fuente importante de aereo-alergenos desencadenantes de asma y rinitis alergica. Objetivo: El proposito de este trabajo fue la produccion y caracterizacion de extractos alergenicos para uso en diagnostico alergologico y terapia. Metodos: Los extractos se prepararon a partir de cultivos puros in vitro de acaros. Las proteinas del acaro fueron extraidas mediante lisis osmotica. La concentracion de proteinas se determino por microensayo de Bradford y el perfil electroforetico fue identificado por SDS-PAGE. La actividad enzimatica fue evaluada con el estuche comercial Api Zym (Biomeriux R y la actividad alergenica por inmunoensayo de Dot Blot. Resultados: Se prepararon cinco lotes de extractos. La media del peso seco de material extraido fue 37,04 mg/gramo de acaros ( DE= 11,3 y la media de la cantidad de proteina fue 212,4 ¿Êg/mg de peso seco (DE= 33,3. El perfil SDS-PAGE mostro la presencia de 8 bandas comunes (21-109 kDa. Todos los extractos presentaron actividad enzimatica hidrolasa. Tambien se demostro actividad tripsina, alfa-amilasa y alfa-quimiotripsina, correspondientes a los alergenos de los acaros del grupo 3, 4 y 6 respectivamente. Todos los extractos tuvieron proteinas con capacidad de union a los anticuerpos IgE. Conclusion: Aqui presentamos la produccion de extractos del acaro DF, con actividad alergenica. Estos resultados fundamentaran la produccion a escala de extractos alergenicos de acaros desencadenantes de enfermedades alergicas en el humano, con fines diagnosticos y de aplicacion terapeutica.

  11. The management of house dust mite allergies

    NARCIS (Netherlands)

    Bronswijk, van J.E.M.H.; Schober, G.; Kniest, F.M.

    1990-01-01

    A safe and practical home sanitation procedure for the removal of house dust mites (Dermatophagoides pteronyssinus) and their allergens is described. The severity of mite infestation was assessed with the use of the Acarex test, which measures the concentration of guanine in house dust, and all

  12. The crystal structure of recombinant proDer p 1, a major house dust mite proteolytic allergen

    DEFF Research Database (Denmark)

    Meno, Kåre; Thorsted, Peter B; Ipsen, Henrik

    2005-01-01

    Allergy to house dust mite is among the most prevalent allergic diseases worldwide. Most house dust mite allergic patients react to Der p 1 from Dermatophagoides pteronyssinus, which is a cysteine protease. To avoid heterogeneity in the sample used for crystallization, a modified recombinant...

  13. Identification of a cross-reactive allergen (presumably tropomyosin) in shrimp, mite and insects

    NARCIS (Netherlands)

    Witteman, A. M.; Akkerdaas, J. H.; van Leeuwen, J.; van der Zee, J. S.; Aalberse, R. C.

    1994-01-01

    A monoclonal antibody to Dermatophagoides pteronyssinus is described that cross-reacts with an IgE-binding antigen present in insects, Crustacea (e.g. shrimp) and other invertebrates. By means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gel filtration and immunofluorescence it was

  14. A microtitre plate radioimmunoassay for the detection and semiquantitation of housedust mite, rye grass pollen and cow's milk specific IgA antibodies

    International Nuclear Information System (INIS)

    Mahoney, G.N.; Hartley, W.A.; Taylor, B.

    1980-01-01

    A microtitre plate based radioimmunoassay (RIA) to measure semiquantitatively allergen specific IgA antibodies is described, with optimal coupling conditions for 3 allergens, house dust mite (Dermatophagoides pteronyssinus), rye grass pollen and cow's milk, and the optimal serum and [ 125 I]anti-IgA incubation conditions. (Auth.)

  15. Rhinitis symptoms and IgE sensitization as risk factors for development of later allergic rhinitis in adults

    DEFF Research Database (Denmark)

    Bødtger, Uffe; Poulsen, L K; Linneberg, A

    2006-01-01

    -examined in 1998. On both occasions questionnaires on rhinitis symptoms were completed and serum IgE (against birch, grass, mugwort, cat, dog, and Dermatophagoides pteronyssinus) were determined (positive if >or=0.35 kUA/l). Asymptomatic sensitization: positive IgE levels without any rhinitis symptoms. Nonallergic...

  16. Molecular, Structural and Immunological Characterization of Der p 18, a Chitinase-Like House Dust Mite Allergen

    Czech Academy of Sciences Publication Activity Database

    Resch, A.; Blatt, K.; Malkus, U.; Fercher, CH.; Swoboda, I.; Focke-Tejkl, M.; Chen, K. W.; Seiberler, S.; Mittermann, I.; Lupinek, CH.; Rodriguez-Dominguez, A.; Zieglmayer, P.; Zieglmayer, R.; Keller, W.; Krzyžánek, Vladislav; Valent, P.; Valenta, R.; Vrtala, S.

    2016-01-01

    Roč. 11, č. 8 (2016), e0160641:1-19 E-ISSN 1932-6203 Institutional support: RVO:68081731 Keywords : dermatophagoides-pteronyssinus * peritrophic matrix * igebinding * protein Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 2.806, year: 2016 http://journals.plos.org/plosone/article/asset?id=10.1371/journal.pone.0160641.PDF

  17. Repellent activities of dichloromethane extract of Allium sativum (garlic) (Liliaceae) against Hyalomma rufipes (Acari).

    Science.gov (United States)

    Nchu, Felix; Magano, Solomon R; Eloff, Jacobus N

    2016-12-02

    Dichloromethane (DCM) extract of garlic (Allium sativum Linn.) bulbs was assessed for its repellent effect against the hard tick, Hyalomma rufipes (Acari: Ixodidae) using two tick behavioural bioassays; Type A and Type B repellency bioassays, under laboratory conditions. These bioassays exploit the questing behaviour of H. rufipes, a tick that in nature displays ambush strategy, seeking its host by climbing up on vegetation and attaching to a passing host. One hundred microlitres (100 µL) of the test solution containing DCM extract of garlic bulbs and DCM at concentrations of 0.35%, 0.7% or 1.4% w/v were evaluated. DCM only was used for control. Tick repellency increased significantly (R2 = 0.98) with increasing concentration (40.03% - 86.96%) yielding an EC50 of 0.45% w/v in Type B repellency bioassay. At concentration of 1.4% w/v, the DCM extract of garlic bulbs produced high repellency index of 87% (male ticks) and 87.5% (female ticks) in the Type A repellency bioassay. Only 4% avoidance of male ticks or female ticks was recorded in the Type B repellency bioassay. In the corresponding controls, the mean numbers of non-repelled male or female ticks were 80% and 41 males or 38 females of 50 ticks in the Type A and Type B repellency bioassays, respectively. The variations in the results could be attributed to the difference in tick repellent behaviours that were assessed by the two repellency bioassays; the Type A repellency bioassay assessed repellent effect of garlic extracts without discriminating between deterrence and avoidance whereas the Type B repellency bioassay only assessed avoidance response. Generally, DCM extract of garlic was repellent against H. rufipes, albeit weak tick repellency was obtained in the Type B repellency bioassay. Furthermore, this study established that the tick repellent activity of garlic extracts is predominantly by deterrence.

  18. Effects of Insecticides and Fungicides Commonly Used in Tomato Production on Phytoseiulus persimilis (Acari: Phtyoseiidae).

    Science.gov (United States)

    Ditillo, J L; Kennedy, G G; Walgenbach, J F

    2016-12-01

    The twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is an important pest of tomatoes in North Carolina. Resident populations of the predatory mite Phytoseiulus persimilis have recently been detected on field-grown tomatoes in central North Carolina, and potentially can be a useful biological control agent against T. urticae Laboratory bioassays were used to assess lethal and reproductive effects of 10 insecticides and five fungicides commonly used in commercial tomato production (chlorantraniliprole, spinetoram, permethrin, imidacloprid, dimethoate, dinotefuran, thiamethoxam, bifenthrin, fenpropathrin, lambda-cyhalothrin, azoxystrobin, chlorothalonil, boscalid, cyazofamid, and mancozeb) on P. persimilis adult females and eggs. Insecticides were tested using concentrations equivalent to 1×, 0.5×, and 0.1× of the recommended field rates. Fungicides were tested at the 1× rate only. Dimethoate strongly impacted P. persimilis with high adult mortality, reduced fecundity, and reduced hatch of eggs laid by treated adults, particularly at high concentrations. The pyrethroids lambda-cyhalothrin, bifenthrin, and fenpropathrin were associated with repellency and reproductive effects at high concentrations. Bifenthrin additionally caused increased mortality at high concentrations. Chlorantraniliprole, dinotefuran, and permethrin did not significantly affect mortality or reproduction. Imidacloprid significantly reduced fecundity and egg viability, but was not lethal to adult P. persimilis Thiamethoxam negatively impacted fecundity at the 1× rate. There were no negative effects associated with fungicide exposure with the exception of mancozeb, which impacted fecundity. Field trials were conducted to explore the in vivo impacts of screened insecticides on P. persimilis populations in the field. Field trials supported the incompatibility of dimethoate with P. persimilis populations. © The Authors 2016. Published by Oxford University Press on behalf

  19. Intercropping Allium tuberosum Rottler ex Sprengel (Amaryllidaceae reduces Tetranychus urticae Koch (Acari: Tetranychidae populations in strawberry

    Directory of Open Access Journals (Sweden)

    Fernando Hata

    2017-12-01

    Efeito do cultivo consorciado de Allium tuberosum Rottlerex Sprengel(Amaryllidaceae em populações de Tetranychus urticae Koch (Acari: Tetranychidae em morangueiro Resumo. O ácaro-rajado, Tetranychus urticae Koch, é uma das mais importantes pragas em casas de vegetação no mundo. Foi avaliado o efeito do consórcio de cebolinha chinesa, cultivada sob as estruturas de madeira que suportam as sacolas para o cultivo de morangueiro (sistema suspenso, em duas casas de vegetação, entre novembro de 2016 e janeiro de 2017. Formas móveis e ovos de ácaros foram contabilizados em folhas de morangueiro. Para as avaliações, cinco plantas foram aleatoriamente selecionadas em cada parcela. Populações de ácaro-rajado em folhas de morangueiro foram avaliadas com lupa de bolso, com 10 x de aumento. Reduções foram observadas em três de oito avaliações de formas móveis de ácaro-rajado. Foram observadas reduções no número de ovos em uma avaliação. Tomando em consideração os dados de ácaros acumulados por dia, foi observado que o consórcio reduziu em 54% a população de ácaros. Por outro lado, o número de ovos não foi alterado.

  20. Effect of Pollen from Different Plant Species on Development of Typhlodromus pyri (Sheuten (Acari: Phytoseiidae Efecto del Polen de Diferentes Especies Vegetales sobre el Desarrollo de Typhlodromus pyri (Sheuten (Acari: Phytoseiidae

    Directory of Open Access Journals (Sweden)

    Paulina Bermúdez

    2010-09-01

    Full Text Available Typhlodromus pyri (Sheuten (Acari: Phytoseiidae is a phytoseiid mite with a high potential in controlling the false Chilean mite (Brevipalpus chilensis Baker; Acari: Tenuipalpidae. The purpose of this study was to determine the effect of different plant species pollen as a complementary food in the development of T. pyri when its prey is in low levels of availability. Mites were individually placed on black plastic boxes with pollen and maintained at a temperature of 26 ± 2 °C, 70 ± 5% relative humidity (RH, and a photoperiod of 16:8 h (L:D. Postembryonic development of T. pyri was studied in 11 pollen species, as well as in a mixed diet of Hirschfeldia incana (L. and B. chilensis. Results show that H. incana was the only pollen in which there was no mortality (P > 0.05 along with the control (Oxalis pes-caprae L.. Mean duration from egg to adult with H. incana was 8.70 ± 1.66 d, protonymph 3.27 ± 0.21 d, and deutonymph 2.90 ± 1.45 d (P > 0.05. The mix feeding of T. pyri did not show any significant differences neither in the mean time from egg to adult, nor in mortality by feeding only with B. chilensis. Survival curves of T. pyri fed only with H. incana pollen, combined with B. chilensis, and only with B. chilensis are higher in the first 14 d of life. The sex ratio was not significantly affected by being fed only with H. incana pollen, B. chilensis, or by a combination of both.Typhlodromus pyri (Sheuten (Acari: Phytoseiidae es un ácaro que presenta un alto potencial de uso para el control de la falsa arañita roja de la vid (Brevipalpus chilensis Baker; Acari: Tenuipalpidae. El objetivo de este estudio fue determinar el efecto del polen de diferentes especies vegetales como alimento complementario para T. pyri cuando escasea su presa. Los parámetros post-embrionarios de T. pyri se estudiaron en 11 especies de polen, en una dieta mixta de polen de Hirschfeldia incana (L. y B. chilensis. Los ácaros se colocaron individualmente sobre

  1. Acari; Tetranychidae

    African Journals Online (AJOL)

    *Corresponding author; E-mail: eziahvin@yahoo.com ... on the Karate®-resistant population showed that the application of these products 1 h before treatment with. Karate® reduce ... tissue, as well as a reduced photosynthetic rate ... Piraneo (2013) was employed to determine ..... toxicity in Norwegian populations of T.

  2. SELETIVIDADE DE INSETICIDAS A Neoseiulus californicus MCGREGOR (ACARI: PHYTOSEIIDAE EM MACIEIRA, NO RIO GRANDE DO SUL AGROCHEMICAL SELECTIVITY TO Neoseiulus californicus MCGREGOR (ACARI: PHYTOSEIIDAE ON APPLE IN RIO GRANDE DO SUL

    Directory of Open Access Journals (Sweden)

    LINO BITTENCOURT MONTEIRO

    2001-12-01

    Full Text Available Testes para determinar os efeitos de agroquímicos homologados para macieira foram realizados em laboratório sobre Neoseiulus californicus (Acari: Phytoseiidae. Os indivíduos testados foram coletados de um pomar comercial da Agriflor Ltda, em Vacaria, Rio Grande do Sul, após várias liberações inoculativas. Os inseticidas utilizados foram os tradicionalmente recomendados para o controle de pragas, principalmente mosca-das-frutas Anastrepha fraterculus (Diptera: Tephritidae. A oviposição e a mortalidade dos ácaros foram avaliadas 12; 24; 48 e 96 horas após a pulverização, cujos produtos foram classificados em quatro classes de toxicidade (IOBC/WPRS. Azinphos ethyl, deltametrina e fenthion provocaram 100% de mortalidade, sendo que dimethoate, fenitrotion, paration, phosmet e triclorfon foram levemente nocivos (classe 2. Malation foi considerado neutro para esta população.The side-effects of agrochemical to Neoseiulus californicus (Acari: Phytoseiidae were studied in laboratory. The mites were collected in commercial apple orchard of Agropastoril Rincão das Flores, in Vacaria, Rio Grande do Sul, Brazil, after successive inoculative releases. The insecticids used were recommended to control of same pest, as Anastrepha fraterculus (Diptera: Tephritidae. The reproduction effect and mortality were evalued 12, 24, 48 and 96 hours after pulverization, while the agrochemical were ranked in toxicity classes, according to IOBC/WPRS. Azinphos ethyl, deltametrina e fenthion were harmful with ca. 100% of mortality in 24 hours, dimethoate, fenitrotion, paration, phosmet e triclorfon were slightly harmful (class 2. Malation was harmless.

  3. TAMBO VIEJO: UN ASENTAMIENTO FORTIFICADO EN EL VALLE DE ACARÍ, PERÚ (Tambo Viejo: A Fortified Settlement in the Acari Valley, Peru

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    Lidio M. Valdez

    2013-10-01

    Full Text Available La aparición de los asentamientos fortificados y el origen del conflicto violento son temas poco discutidos en el contexto de la arqueología peruana. Considero que es oportuno investigar y determinar cuándo y por qué surgieron los primeros asentamientos fortificados. El propósito central de este artículo está precisamente orientado a responder tales interrogantes y discutir el tema del conflicto violento en el valle de Acarí y, por extensión, en la costa sur del Perú. Las evidencias arqueológicas disponibles señalan que, durante el periodo Intermedio Temprano, Tambo Viejo fue un asentamiento defendido por varias estructuras perimétricas. Otros sitios contemporáneos de Tambo Viejo en Acarí también fueron fortificados. Además, existe en el mismo valle evidencia tangible de violencia en la forma de prisioneros que posteriormente fueron decapitados. En contraste a la evidencia proveniente de Acarí, no existen asentamientos del periodo Intermedio Temprano identificables como fortificaciones, lo que hace de los sitios de Acarí los primeros asentamientos fortificados de toda la costa sur. ENGLISH: The emergence of violent conflict and of fortified settlements is a subject little studied within Peruvian archaeology. However, I consider it vital to investigate and determine the time and the reasons under which fortified settlements were first established. The central aim of this paper is to discuss when and why fortified settlements emerged first in the Acari Valley and, by extension, in the south coast of Peru. Available archaeological evidence indicates that during the Early Intermediate Period, Tambo Viejo was a fortified settlement protected by several massive walls. Other neighbouring sites in Acari were also fortified. Furthermore, in Acari there is conclusive evidence for violence in the form of decapitated individuals. In contrast to evidence coming from Acari, not a single Early Intermediate Period settlement from other

  4. Harşit Vadisi’nin (Türkiye) Caligonellid Akarları (Acari: Raphignathoidea: Caligonellidae)

    OpenAIRE

    DOĞAN, Sibel; DOĞAN, Salih; ERMAN, Orhan

    2018-01-01

    Bu çalışmada, Harşit Vadisi’nden (Türkiye) toplanancaligonellid akarların (Acari: Caligonellidae) iki cinsine ait toplam dört türtespit edildi. Türlerden üçü, Caligonellahumilis (Koch), Neognathusspectabilis (Summers ve Schlinger) ve N.terrestris (Summers ve Schlinger), çalışma alanından ilk defa kaydedildi.Bu türlerin, çeşitli organlarının şekilleri çizildi, ölçümleri alındı vetanımları yapıldı. Ayrıca Türkiye’de ve dünyada yayılışları üzerinde duruldu....

  5. Comparing chemical and biological control strategies for twospotted spider mites (Acari: Tetranychidae) in commercial greenhouse production of bedding plants.

    Science.gov (United States)

    Opit, George P; Perret, Jamis; Holt, Kiffnie; Nechols, James R; Margolies, David C; Williams, Kimberly A

    2009-02-01

    Efficacy, costs, and impact on crop salability of various biological and chemical control strategies for Tetranychus urticae Koch (Acari: Tetranychidae) were evaluated on mixed plantings of impatiens, Impatiens wallerana Hook.f (Ericales: Balsaminaceae), and ivy geranium, Pelargonium peltatum (1.) L'Hér. Ex Aiton (Geraniales: Geraniaceae), cultivars in commercial greenhouses. Chemical control consisting of the miticide bifenazate (Floramite) was compared with two biological control strategies using the predatory mite Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae). Treatments were 1) a single, early application of bifenazate; 2) a single, early release of predatory mites at a 1:4 predator:pest ratio based on leaf samples to estimate pest density; 3) a weekly release of predatory mites at numbers based on the area covered by the crop; and 4) an untreated control. T. urticae populations were monitored for 3 wk after the earliest treatment. When plants were ready for market, their salability was estimated. Bifenazate and density-based P. persimilis treatments effectively reduced T. urticae numbers starting 1 wk after plants had been treated, whereas the scheduled, area-based P. persimilis treatment had little or no effect. The percentage of flats that could be sold at the highest market wholesale price ranged from 15 to 33%, 44 to 86%, 84 to 95%, and 92 to 100%, in the control, weekly area-based P. persimilis, bifenazate, and single density-based P. persimilis treatments, respectively. We have shown that in commercial greenhouse production of herbaceous ornamental bedding plants, estimating pest density to determine the appropriate number of predators to release is as effective and offers nearly the same economic benefit as prophylactic use of pesticides.

  6. A novel disease affecting the predatory mite Phytoseiulus persimilis (Acari, Phytoseiidae): 1. Symptoms in adult females.

    Science.gov (United States)

    Schütte, Conny; Kleijn, Prisca W; Dicke, Marcel

    2006-01-01

    Adult female Phytoseiulus persimilis Athias-Henriot (Acari, Phytoseiidae) of one of our laboratory populations showed a lower degree of attraction to herbivore-induced plant volatiles than other laboratory populations. We hypothesized earlier that this consistent change in foraging behavior is a symptom of a disease, as it is a contagious phenomenon. Here we describe more symptoms by comparing mated females of this population (non-responding (NR) population) with mated females of other populations that are strongly attracted to herbivore-induced plant volatiles (responding populations). The most apparent characteristic of the NR population was the presence of numerous dorso-ventrally flattened females (76% of all females). These females had a normal size after mating but shrank during adulthood. Independent of their age, shrunken females did not reproduce and died a few days after shrinking. In addition to these profound differences in short term performance, females from the NR-population showed behavioral changes, including a lower degree of attraction to herbivore-induced plant volatiles, a higher tendency to leave a prey-patch and a lower predation rate. Moreover, about half of the live females of the NR-population carried birefringent dumbbell-shaped crystals in the legs whereas live females of a responding population carried crystals only in the lumen of the Malpighian tubules and the rectum. The symptom 'crystals in the legs' was correlated with low reproduction. Energy dispersive X-ray diffraction of these crystals revealed that they contain calcium and phosphorus along with carbon and oxygen. Crystals with comparable elemental compositions and the same characteristic concentric layering are well known in insects, where they are thought to play a major role in detoxification of calcium and heavy metals, and in storage of phosphorus. The fraction of predators carrying a white spot in the distal part of the opisthosoma, due to accumulation of excretory

  7. Ticks (Acari: Ixodidae as ectoparasites of Brazilian wild birds and their association with rickettsial diseases

    Directory of Open Access Journals (Sweden)

    Maria Ogrzewalska

    2016-04-01

    Full Text Available Os carrapatos (Acari: Ixodoidea são ectoparasitas obrigatórias de uma variedade de hospedeiros vertebrados e têm um papel importante na ecologia e transmissão de diversos patógenos causadores de doenças em humanos e animais no mundo todo. No Brasil existem 68 espécies de carrapatos e pelo menos 23 espécies são encontradas parasitando aves silvestres. Esse número tem crescido com o advento de novos estudos ressaltando o papel das aves nos ciclos de vida desses artrópodes. Na América do Sul alguns desses carrapatos estão envolvidos na epidemiologia de doenças graves para o ser humano, como a febre maculosa, causada por bactérias do gênero Rickettsia (Rickettsiales: Rickettsiaceae. O alvo desse artigo é apresentar o estado atual de conhecimento sobre a fauna de carrapatos encontrados em aves no Brasil e as associações estabelecidas com as riquetsioses. A literatura disponível sobre carrapatos em aves e ocorrência de riquétsias foi revisada e pôde ser concluído que aves têm um papel importante nos ciclos de vida de várias espécies de carrapatos, sendo especialmente importantes para os estágios imaturos (larvas e ninfas. A maior parte do conhecimento sabrecai na fauna de carrapatos de aves dos biomas Mata Atlântica e Cerrado no sudeste e centro-oeste do Brasil. Já o conhecimento sobre o parasitismo por carrapatos em aves dos outros biomas: Amazônia, Caatinga, Pantanal e Pampas é muito limitado. Além disso, não há estudos sobre o papel de aves como disseminadores de carrapatos entre áreas e também o papel de aves no ciclo de Rickettsia não está totalmente esclarecido.

  8. Ecological relationships between xerophilic fungi and house-dust mites (Acarida: Pyroglyphidae).

    Science.gov (United States)

    Lustgraaf, B V D

    1978-01-01

    At. 75 and 80% relative humidity (RH), on a wheat germ flake medium, Aspergillus penicilloides grew abundantly and suppressed the population growth of Dermatophagoides pteronyssiunus. At 71% RH, A. penicilloides grew moderately and was only antagonistic to D. pteronyssinus when the fungus was previously incubated on the medium.On a human dander medium and on mattress dust, A. penicilloides grew moderately at 71% and 75% RH and stimulated the development of D. pteronyssinus populations. Also a moderate growth of Eurotium repens on human dander positively influenced D. pteronyssinus. Wallemia sebi and Penicillium brevicompactum grew slightly or did not grow at all at 75% RH. No effect was observed on D. pteronyssinus.It appears that xerophilic fungi may stimulate, and occasionally may reduce, the growth of house-dust mite populations in the natural environment.

  9. A revision of the family Ameroseiidae (Acari, Mesostigmata), with some data on Slovak fauna

    Science.gov (United States)

    Mašán, Peter

    2017-01-01

    Abstract The family Ameroseiidae Evans, 1961 (Acari: Mesostigmata) includes a total of 12 valid and adequately described genera, namely Afrocypholaelaps Elsen, 1972, Ameroseiella Bregetova, 1977, Ameroseius Berlese, 1904, Asperolaelaps Womersley, 1956, Brontispalaelaps Womersley, 1956, Epicriopsis Berlese, 1916, Hattena Domrow, 1963, Kleemannia Oudemans, 1930, Neocypholaelaps Vitzthum, 1942, Pseudoameroseius gen. n., Sertitympanum Elsen & Whitaker, 1985 and Sinoseius Bai & Gu, 1995. One of these genera includes subgenera, namely Kleemannia (Primoseius) Womersley, 1956. All genera are reviewed and re-diagnosed, and a dichotomous key is provided for their identification. Ameroseius (50 species), Kleemannia (28 species) and Neocypholaelaps (22 species) are the largest genera in the family. Ameroseiella, Kleemannia, Kleemannia (Primoseius) and Sinoseius are considered to be valid taxa and, in presented systematic classification, they are removed from synonymy with Ameroseius. The genus Pseudoameroseius gen. n., with type species Ameroseius michaelangeli Moraza, 2006 (from Canary Islands), is newly erected to further refine broad primary concept of Ameroseius as understood by some former authors (Karg, Bregetova). Asperolaelaps is removed from synonymy with Neocypholaelaps. Three new species are here described, namely Ameroseius renatae sp. n. (based on specimens from Slovakia), Kleemannia dolichochaeta sp. n. (from Spain) and Kleemannia miranda sp. n. (from U.S.A.). The following new junior synonymies are proposed: Ameroseius apodius Karg, 1971 = Ameroseiella macrochelae (Westerboer, 1963); Ameroseius bregetovae Livshits & Mitrofanov, 1975 = Neocypholaelaps favus Ishikawa, 1968; Ameroseius chinensis Khalili-Moghadam & Saboori, 2016 = Ameroseius guyimingi Ma, 1997; Ameroseius crassisetosus Ye & Ma, 1993, Ameroseius qinghaiensis Li & Yang, 2000 and Ameroseius norvegicus Narita, Abduch & Moraes, 2015 = Ameroseius corbiculus (Sowerby, 1806); Ameroseius dubitatus Berlese

  10. A revision of the family Ameroseiidae (Acari, Mesostigmata, with some data on Slovak fauna

    Directory of Open Access Journals (Sweden)

    Peter Mašán

    2017-09-01

    Full Text Available The family Ameroseiidae Evans, 1961 (Acari: Mesostigmata includes a total of 12 valid and adequately described genera, namely Afrocypholaelaps Elsen, 1972, Ameroseiella Bregetova, 1977, Ameroseius Berlese, 1904, Asperolaelaps Womersley, 1956, Brontispalaelaps Womersley, 1956, Epicriopsis Berlese, 1916, Hattena Domrow, 1963, Kleemannia Oudemans, 1930, Neocypholaelaps Vitzthum, 1942, Pseudoameroseius gen. n., Sertitympanum Elsen & Whitaker, 1985 and Sinoseius Bai & Gu, 1995. One of these genera includes subgenera, namely Kleemannia (Primoseius Womersley, 1956. All genera are reviewed and re-diagnosed, and a dichotomous key is provided for their identification. Ameroseius (50 species, Kleemannia (28 species and Neocypholaelaps (22 species are the largest genera in the family. Ameroseiella, Kleemannia, Kleemannia (Primoseius and Sinoseius are considered to be valid taxa and, in presented systematic classification, they are removed from synonymy with Ameroseius. The genus Pseudoameroseius gen. n., with type species Ameroseius michaelangeli Moraza, 2006 (from Canary Islands, is newly erected to further refine broad primary concept of Ameroseius as understood by some former authors (Karg, Bregetova. Asperolaelaps is removed from synonymy with Neocypholaelaps. Three new species are here described, namely Ameroseius renatae sp. n. (based on specimens from Slovakia, Kleemannia dolichochaeta sp. n. (from Spain and Kleemannia miranda sp. n. (from U.S.A.. The following new junior synonymies are proposed: Ameroseius apodius Karg, 1971 = Ameroseiella macrochelae (Westerboer, 1963; Ameroseius bregetovae Livshits & Mitrofanov, 1975 = Neocypholaelaps favus Ishikawa, 1968; Ameroseius chinensis Khalili-Moghadam & Saboori, 2016 = Ameroseius guyimingi Ma, 1997; Ameroseius crassisetosus Ye & Ma, 1993, Ameroseius qinghaiensis Li & Yang, 2000 and Ameroseius norvegicus Narita, Abduch & Moraes, 2015 = Ameroseius corbiculus (Sowerby, 1806; Ameroseius dubitatus Berlese

  11. Influence of the webbing produced by Oligonychus ilicis (McGregor) (Acari: Tetranychidae) on associated predatory phytoseiids; Influencia da teia de Oligonychus ilicis (McGregor) (Acari: Tetranychidae) sobre os fitoseideos predadores associados

    Energy Technology Data Exchange (ETDEWEB)

    Franco, Renato A. [Bioagri Laboratorios Ltda., Piracicaba, SP (Brazil)], e-mail: r.franco@bioagri.com.br; Reis, Paulo R. [Empresa de Pesquisa Agropecuaria de Minas Gerais (EPAMIG), Belo Horizonte, MG (Brazil). Ecocentro], e-mail: paulo.rebelles@epamig.ufla.br; Zacarias, Mauricio S. [EMBRAPA Cafe, Lavras, MG (Brazil)], e-mail: zacarias@epamig.ufla.br; Oliveira, Daniel C. [Universidade Federal de Lavras (UFLA), MG (Brazil)

    2010-01-15

    Oligonychus ilicis (McGregor) is among those mite species that can cause damage to coffee plants (Coffea spp.). Species of Phytoseiidae acari are considered the most important and studied predatory mites. The objective of this study was to evaluate the effects of the webbing produced by O. ilicis on its predation by females of the phytoseiids Iphiseiodes zuluagai Denmark and Muma, Euseius citrifolius Denmark and Muma and Amblyseius herbicolus (Chant). Four bioassays were conducted, with three treatments and ten replicates. Each replicate consisted of 25 O. ilicis per experimental unit (a leaf disc of Coffea arabica) according to the tested developmental stage, in independent experiments. To spin the web, 15 adult females were put on each experimental unit for 24h; females were then removed, leaving only the web, and predators and prey to be tested were introduced. Predation was assessed after 24h. In the presence of webbing, the consumption of eggs, larvae and nymphs by I. zuluagai and eggs and larvae by E. citrifolius was lower. For A. herbicolus, egg predation was lower, but larval predation did not vary significantly and predation of nymphs and adults was higher in the presence of webbing. Predators as a whole were more efficient consuming larvae regardless of the presence of webbing. Considering the stages of O. ilicis altogether, webbing reduced the predation potential of I. zuluagai and E. citrifolius, but not of A. herbicolus. (author)

  12. Prevalencia de la sensibilización a tres ácaros domésticos en la población infantil alérgica de un consultorio médico Prevalence of sensitization to three house dust mites in the allergic infantile population of a family physician's office

    Directory of Open Access Journals (Sweden)

    Mayda González León

    2005-04-01

    Full Text Available Se realizó un estudio con el objetivo de investigar si la sensibilización a estos ácaros ( Dermatophagoides pteronyssinus [ Dp ], Dermatophagoides siboney [ Ds ] y Blomia tropicalis [ Bt ] constituye un problema de salud en nuestra población infantil, pues el estudio de los problemas alérgicos ha identificado a los ácaros domésticos como los principales agentes causales de asma y otras afecciones. Se seleccionaron 32 niños alérgicos de nuestro consultorio que se clasificaron según su grado de exposición al polvo doméstico. A todos se les realizó la prueba por punción cutánea en el brazo derecho, y se utilizaron 3 extractos alergénicos estandarizados Valergen Ò ( Blomia tropicalis, Dermatophagoides siboney y Dermatophagoides pteronyssinus de producción nacional (BIOCEN. La prevalencia de la sensibilización fue mayor de forma significativa (pA study was conducted aimed at investigating if the sensitization to these dust mites (Dermatophagoides pteronyssinus [Dp], Dermatophagoides siboney [Ds] and Blomia tropicalis [Bt] is a health problem in our infantile population, since the study of the allergic problems has identified the house dust mites as the main agents causing asthma and other affections. 32 allergic children of our office were selected and classified according to their degree of exposure to house dust. All of them underwent the skin test in the right arm. 3 standardized allergenic extracts ValergenÒ (Blomia tropicalis, Dermatophagoides siboney y Dermatophagoides pteronyssinus of national production (BIOCEN were used. The prevalence of sensitization was significantly higher (p<0.05 for all the dust mites in patients with a greater exposure to house dust compared with those with lower exposure to it. A higher sensitization was attained for the dust mites of the genus Dermatophagoides. The extract of Dp produced a wheal with a mean size greater than the one caused by other products.

  13. Air temperature optimisation for humidity-controlled cold storage of the predatory mites Neoseiulus californicus and Phytoseiulus persimilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Ghazy, Noureldin Abuelfadl; Suzuki, Takeshi; Amano, Hiroshi; Ohyama, Katsumi

    2014-03-01

    Humidity-controlled cold storage, in which the water vapour pressure is saturated, can prolong the survival of the predatory mites Neoseiulus californicus (McGregor) and Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae). However, information on the optimum air temperature for long-term storage by this method is limited. The authors evaluated the survival of mated adult females of N. californicus and P. persimilis at 5.0, 7.5, 10.0 and 12.5 °C under saturated water vapour condition (vapour pressure deficit 0.0 kPa). N. californicus showed a longer survival time than P. persimilis at all the air temperatures. The longest mean survival time of N. californicus was 11 weeks at 7.5 °C, whereas that of P. persimilis was 8 weeks at 5.0 °C. After storage at 7.5 °C for 8 weeks, no negative effect on post-storage oviposition was observed in N. californicus, whereas the oviposition of P. persimilis stored at 5.0 °C for 8 weeks was significantly reduced. The interspecific variation in the response of these predators to low air temperature might be attributed to their natural habitat and energy requirements. These results may be useful for the long-term storage of these predators, which is required for cost-effective biological control. © 2013 Society of Chemical Industry.

  14. Transcriptome Analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867 (Acari: Tetranychidae, and Its Response to β-Sitosterol

    Directory of Open Access Journals (Sweden)

    Chunya Bu

    2015-01-01

    Full Text Available Tetranychus cinnabarinus (Acari: Tetranychidae is a worldwide polyphagous agricultural pest that has the title of resistance champion among arthropods. We reported previously the identification of the acaricidal compound β-sitosterol from Mentha piperita and Inula japonica. However, the acaricidal mechanism of β-sitosterol is unclear. Due to the limited genetic research carried out, we de novo assembled the transcriptome of T. cinnabarinus using Illumina sequencing and conducted a differential expression analysis of control and β-sitosterol-treated mites. In total, we obtained >5.4 G high-quality bases for each sample with unprecedented sequencing depth and assembled them into 22,941 unigenes. We identified 617 xenobiotic metabolism-related genes involved in detoxification, binding, and transporting of xenobiotics. A highly expanded xenobiotic metabolic system was found in mites. T. cinnabarinus detoxification genes—including carboxyl/cholinesterase and ABC transporter class C—were upregulated after β-sitosterol treatment. Defense-related proteins, such as Toll-like receptor, legumain, and serine proteases, were also activated. Furthermore, other important genes—such as the chloride channel protein, cytochrome b, carboxypeptidase, peritrophic membrane chitin binding protein, and calphostin—may also play important roles in mites’ response to β-sitosterol. Our results demonstrate that high-throughput-omics tool facilitates identification of xenobiotic metabolism-related genes and illustration of the acaricidal mechanisms of β-sitosterol.

  15. Influence of the webbing produced by Oligonychus ilicis (McGregor) (Acari: Tetranychidae) on associated predatory phytoseiids

    International Nuclear Information System (INIS)

    Franco, Renato A.; Reis, Paulo R.; Oliveira, Daniel C.

    2010-01-01

    Oligonychus ilicis (McGregor) is among those mite species that can cause damage to coffee plants (Coffea spp.). Species of Phytoseiidae acari are considered the most important and studied predatory mites. The objective of this study was to evaluate the effects of the webbing produced by O. ilicis on its predation by females of the phytoseiids Iphiseiodes zuluagai Denmark and Muma, Euseius citrifolius Denmark and Muma and Amblyseius herbicolus (Chant). Four bioassays were conducted, with three treatments and ten replicates. Each replicate consisted of 25 O. ilicis per experimental unit (a leaf disc of Coffea arabica) according to the tested developmental stage, in independent experiments. To spin the web, 15 adult females were put on each experimental unit for 24h; females were then removed, leaving only the web, and predators and prey to be tested were introduced. Predation was assessed after 24h. In the presence of webbing, the consumption of eggs, larvae and nymphs by I. zuluagai and eggs and larvae by E. citrifolius was lower. For A. herbicolus, egg predation was lower, but larval predation did not vary significantly and predation of nymphs and adults was higher in the presence of webbing. Predators as a whole were more efficient consuming larvae regardless of the presence of webbing. Considering the stages of O. ilicis altogether, webbing reduced the predation potential of I. zuluagai and E. citrifolius, but not of A. herbicolus. (author)

  16. Laboratory evaluation of a native strain of Beauveria bassiana for controlling Dermanyssus gallinae (De Geer, 1778) (Acari: Dermanyssidae).

    Science.gov (United States)

    Immediato, Davide; Camarda, Antonio; Iatta, Roberta; Puttilli, Maria Rita; Ramos, Rafael Antonio Nascimento; Di Paola, Giancarlo; Giangaspero, Annunziata; Otranto, Domenico; Cafarchia, Claudia

    2015-09-15

    The poultry red mite, Dermanyssus gallinae (De Geer, 1778) (Acari: Dermanyssidae) is one of the most economically important ectoparasites of laying hens worldwide. Chemical control of this mite may result in environmental and food contamination, as well as the development of drug resistance. High virulence of Beauveria bassiana sensu lato strains isolated from naturally infected hosts or from their environment has been demonstrated toward many arthropod species, including ticks. However, a limited number of studies have assessed the use of B. bassiana for the control of D. gallinae s.l. and none of them have employed native strains. This study reports the pathogenicity of a native strain of B. bassiana (CD1123) against nymphs and adults of D. gallinae. Batches of nymph and adult mites (i.e., n=720 for each stage) for treated groups (TGs) were placed on paper soaked with a 0.1% tween 80 suspension of B. bassiana (CIS, 10(5), 10(7) and 10(9) conidia/ml), whilst 240 untreated control mites for each stage (CG) were exposed only to 0.1% tween 80. The mites in TG showed a higher mortality at all stages (pbassiana suspension containing 10(9) conidia/ml was highly virulent towards nymph and adult stages of D. gallinae, therefore representing a possible promising natural product to be used in alternative or in combination to other acaricidal compounds currently used for controlling the red mite. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Impact of ectohumus application in birch and pine nurseries on the presence of soil mites (Acari, Oribatida in particular

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    Klimek Andrzej

    2016-03-01

    Full Text Available Intensively used forest nurseries are characterised by degradation processes that lead to a drop in the quality of seedlings. The main reason of this problem is a decrease in biological soil diversity. Therefore, an attempt of nursery soil enrichment by introducing ectohumus – as compost and fresh litter – from the pine forest was carried out. The research was carried out in 2009–2011 in the Bielawy forest nursery near the city of Toruń, Poland. The objective of the study was to determine the impact of organic fertilisation (compost made up of forest humus and mulching using fresh ectohumus on the density and community composition of Acari mites and on species composition of oribatid mites (Oribatida in the nurseries of silver birch and Scots pine. Mites, especially oribatid mites, were treated as bioindicators of soil biological activity. Research has shown that mulching using fresh ectohumus caused a multiple increase in the density of mites, especially in saprophagous mites Oribatida. Oribatid mites were clearly more numerous in birch cultivation than in that of pine. Overall, 27 species of oribatid mites were found. Mulching resulted in a significant growth in species diversity in both cultivations. The most numerous oribatid mite in the area under the study was Oribatula tibialis. This species was present in all plots and showed clear preference for birch cultivation. Tectocepheus velatus and Oppiella nova, common and known to be present in a variety of environments, were slightly less numerous.

  18. Compatibility of reduced-risk insecticides with the non-target predatory mite Iphiseius degenerans (Acari: Phytoseiidae).

    Science.gov (United States)

    Döker, İsmail; Pappas, Maria L; Samaras, Konstantinos; Triantafyllou, Anneta; Kazak, Cengiz; Broufas, George D

    2015-09-01

    Iphiseius degenerans (Berlese) (Acari: Phytoseiidae) is a common predatory mite in citrus orchards in some areas of the Mediterranean basin and an important biological control agent of the thrips Frankliniella occidentalis (Pergande) in Integrated Pest Management (IPM) programmes in greenhouse crops. In this study, we evaluated the effects of the 'reduced-risk' insecticides acetamiprid, chlorantraniliprole, flubendiamide, metaflumizone, methoxyfenozide, spinetoram and thiamethoxam on I. degenerans, as a means of testing their compatibility in IPM programmes. Although all pesticides decreased immature survival, high mortality was only recorded for young larvae when exposed to acetamiprid, while metaflumizone, thiamethoxam and spinetoram resulted in intermediate lethal effects. The estimated LC50 values of acetamiprid, spinetoram and thiamethoxam for I. degenerans females were 0.52, 0.84 and 0.16-fold lower than the respective maximum recommended doses of the pesticides for field application. Although all pesticides tested significantly decreased fecundity, highest rates corresponded to the three pesticides already mentioned. Chlorantraniliprole, flubendiamide and methoxyfenozide may preliminarily be included in IPM programmes, whereas the effects of acetamiprid, metaflumizone, spinetoram and thiamethoxam on I. degenerans should be clarified in further field toxicological tests. This information could be useful for rationally planning and implementing pest management on a sustainable basis. © 2014 Society of Chemical Industry.

  19. Community structure variability of Uropodina mites (Acari: Mesostigmata) in nests of the common mole, Talpa europaea, in Central Europe.

    Science.gov (United States)

    Napierała, Agnieszka; Mądra, Anna; Leszczyńska-Deja, Kornelia; Gwiazdowicz, Dariusz J; Gołdyn, Bartłomiej; Błoszyk, Jerzy

    2016-04-01

    Underground nests of Talpa europaea, known as the common mole, are very specific microhabitats, which are also quite often inhabited by various groups of arthropods. Mites from the suborder Uropodina (Acari: Mesostigmata) are only one of them. One could expect that mole nests that are closely located are inhabited by communities of arthropods with similar species composition and structure. However, results of empirical studies clearly show that even nests which are close to each other can be different both in terms of the species composition and abundance of Uropodina communities. So far, little is known about the factors that can cause these differences. The major aim of this study was to identify factors determining species composition, abundance, and community structure of Uropodina communities in mole nests. The study is based on material collected during a long-term investigation conducted in western parts of Poland. The results indicate that the two most important factors influencing species composition and abundance of Uropodina communities in mole nests are nest-building material and depth at which nests are located. Composition of Uropodina communities in nests of moles was also compared with that of other microhabitats (e.g. rotten wood, forest litter, soil) based on data from 4421 samples collected in Poland. Communities of this habitat prove most similar to these of open areas, especially meadows, as well as some forest types.

  20. The international trade in reptiles (Reptilia)--the cause of the transfer of exotic ticks (Acari: Ixodida) to Poland.

    Science.gov (United States)

    Nowak, Magdalena

    2010-05-11

    The problem of the unnatural transfer of exotic ticks (Acari: Ixodida) on reptiles (Reptilia) imported to Poland is presented. In the period from 2003 to 2007, 382 specimens of reptiles belonging to the following genera were investigated: Testudo, Iguana, Varanus, Gongylophis, Python, Spalerosophis, Psammophis. The reptiles most infested with ticks are imported to Poland from Ghana in Africa, and are the commonly bred terrarium reptiles: Varanus exanthematicus and Python regius. As a result of the investigations, the transfer of exotic ticks on reptiles to Poland was confirmed. There were 2104 specimens of the genera Amblyomma and Hyalomma. The following species were found: Amblyomma exornatum Koch, 1844, Amblyomma flavomaculatum (Lucas, 1846), Amblyomma latum Koch, 1844, Amblyomma nuttalli Donitz, 1909, Amblyomma quadricavum (Schulze, 1941), Amblyomma transversale (Lucas, 1844), Amblyomma varanense (Supino, 1897), Amblyomma sp. Koch, 1844, Hyalomma aegyptium (Linnaeus, 1758). All the species of ticks of genus Amblyomma revealed have been discovered in Poland for the first time. During the research, 13 cases of anomalies of morphological structure were confirmed in the ticks A. flavomaculatum, A. latum and H. aegyptium. The expanding phenomenon of the import of exotic reptiles in Poland and Central Europe is important for parasitological and epidemiological considerations, and therefore requires monitoring and wide-ranging prophylactic activities to prevent the inflow of exotic parasites to Poland. (c) 2010 Elsevier B.V. All rights reserved.

  1. Development and reproductive potential of Tyrophagus putrescentiae (Acari: Acaridae) on plant-parasitic nematodes and artificial diets.

    Science.gov (United States)

    Abou El-Atta, Doaa Abd El-Maksoud; Osman, Mohamed Ali

    2016-04-01

    This study investigated development, reproduction and life table parameters of the astigmatid mold mite Tyrophagus putrescentiae (Schrank) (Acari: Acaridae) feeding on egg-masses or adult females of the nematode Meloidogyne incognita, egg-masses of the nematode Rotylenchulus reniformis, ras cheese or yeast at 25 ± 1 °C, 70 ± 10 % RH in the dark. Immature developmental times were shorter when the mite was fed females of M. incognita followed by yeast. Different prey/diet types had no significant effect on longevity and lifespan of both males and females. Daily oviposition rate (eggs/female/day) was highest for mites fed yeast (20.8 ± 1.8 eggs) and lowest for mites fed females of M. incognita (6.6 ± 0.5). Intrinsic rate of natural increase (r m) was highest for mites fed yeast compared to other prey/diet; no significant differences in r m were observed among mites fed on non-yeast diets. This result may suggest a role of T. putrescentiae as biocontrol agent of plant-parasitic nematodes and the yeast may be used for mite mass-production purposes.

  2. Regional factors rather than forest type drive the community structure of soil living oribatid mites (Acari, Oribatida).

    Science.gov (United States)

    Erdmann, Georgia; Scheu, Stefan; Maraun, Mark

    2012-06-01

    Most European forests are managed by humans. However, the manner and intensity of management vary. While the effect of forest management on above-ground communities has been investigated in detail, effects on the below-ground fauna remain poorly understood. Oribatid mites are abundant microarthropods in forest soil and important decomposers in terrestrial ecosystems. Here, we investigated the effect of four forest types (i.e., managed coniferous forests; 30 and 70 years old managed beech forests; natural beech forests) on the density, diversity and community structure of oribatid mites (Acari). The study was replicated at three regions in Germany: the Swabian Alb, the Hainich and the Schorfheide. To relate changes in oribatid mite community structure to environmental factors, litter mass, pH, C and N content of litter, fine roots and C content of soil were measured. Density of oribatid mites was highest in the coniferous forests and decreased in the order 30 years old, 70 years old, and natural beech forests. Mass of the litter layer and density of oribatid mites were strongly correlated indicating that the litter layer is an important factor regulating oribatid mite densities. Diversity of oribatid mites was little affected by forest type indicating that they harbor similar numbers of niches. Species composition differed between the forest types, suggesting different types of niches. The community structure of oribatid mites differed more strongly between the three regions than between the forest types indicating that regional factors are more important than effects associated with forest type.

  3. Efficacy of plant-derived and synthetic compounds on clothing as repellents against Ixodes scapularis and Amblyomma americanum (Acari: Ixodidae).

    Science.gov (United States)

    Jordan, Robert A; Schulze, Terry L; Dolan, Marc C

    2012-01-01

    We conducted field trials to compare the relative repellent activity of two natural product compounds (nootkatone and carvacrol) with commercially available plant-derived (EcoSMART organic insect repellent) and permethrin-based (Repel Permanone) repellents against adult Ixodes scapularis Say and Amblyomma americanum (L.) (Acari: Ixodidae) by using treated coveralls. One day after treatment, nootkatone and carvacrol provided 100% repellency of I. scapularis adults, with nootkatone maintaining complete protection through 3 d, whereas carvacrol showed steadily declining repellency against I. scapularis during the 7-d course of the trials. Nootkatone was at least as effective against host-seeking A. americanum as against I. scapularis through 3 d. Carvacrol provided little protection against A. americanum adults. Both natural compounds performed well initially in comparison with the commercial products. After 7 d, nootkatone was the most effective against both species followed in order of activity by Permanone, EcoSMART, and carvacrol. Nootkatone seems to have offer considerable potential as a clothing repellent against both I. scapularis and A. americanum.

  4. Life history of Neoseiulus californicus (McGregor, 1954 (Acari: Phytoseiidae fed with castor bean (Ricinus communisL. pollen in laboratory conditions

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    PP Marafeli

    Full Text Available The predatory mite, Neoseiulus californicus(McGregor, 1954 (Acari: Phytoseiidae is one of the principal natural enemies of tetranychid mites in several countries, promoting efficient control of those mites in several food and ornamental crops. Pest attacks such as that of the spider mite, Tetranychus urticaeKoch, 1836 (Acari: Tetranychidae, is one of the problems faced by farmers, especially in the greenhouse, due to the difficulty of its control with the use of chemicals because of the development of fast resistance making it hard to control it. The objective of this work was to study the life history of the predatory mite N. californicus as a contribution to its mass laboratory rearing, having castor bean plant [Ricinus communis L. (Euphorbiaceae] pollen as food, for its subsequent use as a natural enemy of T. urticae on a cultivation of greenhouse rosebushes. The studies were carried out in the laboratory, at 25 ± 2°C of temperature, 70 ± 10% RH and a 14 hour photophase. The biological aspects and the fertility life table were appraised. Longevity of 32.9 days was verified for adult females and 40.4 days for males. The intrinsic rate of increase (rm was 0.2 and the mean generation time (T was 17.2 days. The population doubled every 4.1 days. The results obtained were similar to those in which the predatory mite N. californicus fed on T. urticae.

  5. Experimental infestation with the immatures of Amblyomma dissimile Koch, 1844 (Acari: Ixodidae on Tropidurus torquatus (Lacertilia: Iguanidae and Oryctolagus cuniculus Infestação experimental com as fases imaturas de Amblyomma dissimile Koch, 1844 (Acari: Ixodidae em Tropidurus torquatus (Lacertilia: Iguanidae e Oryctolagus cuniculus

    Directory of Open Access Journals (Sweden)

    L.H.T. Freitas

    2004-02-01

    Full Text Available Larvas provenientes de duas fêmeas de Amblyomma dissimile Koch, 1844 (Acari: Ixodidae, naturalmente ingurgitadas em uma iguana (Iguana iguana e provenientes do Estado do Mato Grosso, foram utilizadas na infestação experimental de lagartos da espécie Tropidurus torquatus e coelhos domésticos. As larvas alimentadas em ambos os hospedeiros realizaram ecdise para ninfas. As ninfas apenas ingurgitaram no lagarto e mudaram para machos e fêmeas. Este é o primeiro registro do parasitismo de larvas e ninfas de A. dissimile em T. torquatus e de larvas em coelhos.

  6. Seletividade de produtos fitossanitários sobre o ácaro predador Agistemus brasiliensis Matioli, Ueckermann & Oliveira (Acari: Stigmaeidae Selectivity ofthe pesticides tothe predaceous mite Agistemus brasiliensis Matioli,, Ueckermann & Oliveira (Acari: Stigmaeidae

    Directory of Open Access Journals (Sweden)

    Marcos Zatti da Silva

    2009-06-01

    Full Text Available Os ácaros predadores das famílias Phytoseiidae e Stigmaeidae constituem-se nos principais inimigos naturais de Brevipalpus phoenicis (Geijskes em citros. Este ácaro-praga causa sérios prejuízos na produção, devido à transmissão do vírus da leprose dos citros (CiLV. Apesar do grande volume de informações sobre a sensibilidade de ácaros Phytoseiidae a agrotóxicos, praticamente não existem informações sobre o efeito desses compostos em ácaros Stigmaeidae no Brasil. Sendo assim, o trabalho teve por objetivo avaliar o efeito dos principais agrotóxicos utilizados em citros sobre o ácaro predador Agistemus brasiliensis Matioli, Ueckermann & Oliveira (Acari: Stigmaeidae, em condições de laboratório. Arenas de folhas de citros da variedade Pera, contendo 25 fêmeas adultas de A. brasiliensis, foram pulverizadas em torre de Potter. Avaliaram-se as mortalidades dos ácaros 72 horas após a aplicação. O efeito dos produtos na reprodução do acarino e a viabilidade dos ovos também foram avaliados. Quanto à seletividade, conforme proposta da "Organização Internacional para o Controle Biológico" (IOBC, os produtos foram classificados como: classe 1 - inócuo (E99%, calda sulfocálcica, cyhexatin, flufenoxuron, hexythiazox, óxido de fenbutatin, propargite, pyridaben e spirodiclofen. Estudos conduzidos em condições de campo ainda são necessários para se compreender melhor o efeito desses agrotóxicos sobre o ácaro predador.The predaceous mites of the families Phytoseiidae and Stigmaeidae are the most important natural enemies of Brevipalpus phoenicis (Geijskes on citrus. This mite causes serious damages to the yield due to the transmission of Citrus leprosis virus (CiLV. Despite the considerable amount of information on susceptibility of phytoseiids to pesticides, the effect of these compounds is not very known for stigmaeid mites in Brazil. This work was carried out to evaluate the effect of the main pesticides used in

  7. New records of water mites of the family Torrenticolidae (Acari, Hydrachnidia) with descriptions of two new species from Nanshih River system in Taiwan and redescription of Torrenticola ussuriensis (Sokolow, 1940) from the Russian Far East

    Science.gov (United States)

    Pešić, Vladimir; Semenchenko, Ksenia A.; Chatterjee, Tapas; Yam, Rita S.W.; Chan, Benny K.K.

    2011-01-01

    Abstract New records of torrenticolid water mites (Acari: Hydrachnidia, Torrenticolidae) from Nanshih River, Taiwan, are presented. Two new species are described: Torrenticola nanshihensis and Torrenticola taiwanicus; the latter species is compared with Torrenticola ussuriensis (Sokolow, 1940), a poorly known species which is re-described based on a new material from the Russian Far East; Monatractides cf. circuloides (Halík, 1930)is reported for the first time for Taiwan. PMID:21998497

  8. Distribution and Seasonal Activity of Hard Ticks (Acari: Ixododae Infesting Domestic Ruminants in Famenin County, Hamadan Province, Iran

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    F. Fayazkhoo

    2017-10-01

    Full Text Available Background and Objective: Hard ticks (Acari: Ixodidae are one of the most important obligate ectoparasites of vertebrates, belonging to class Arachnida, which transmit a wide range of pathological agents such as viruses, bacteria, and parasites to humans and animals in Iran and around the world. Identifying the distribution of hard ticks in a region is important to monitor their control program, and thereby prevent disease transmission. Materials and Methods: In this cross-sectional descriptive study, sampling was carried out from different parts of the livestock body during four seasons in four geographical directions and five villages of Famenin County, Hamadan Province, Iran, during 2015-2016. The ticks were initially stimulated by using chloroform solution and then separated from domestic ruminants by forceps. The collected ticks were sent to a laboratory, and then their sex and species were identified by using valid diagnostic keys. Results: We studied 800 domestic ruminants, including cattle, sheep, and goats, and found 150 (18.7% cases of infestation. A total of 274 ticks were collected, 259 of which were hard ticks including four genera of Hyalomma, Dermacentor, Repicephlus, and Haemaphysalis. The greatest diversity of species, including Hyalomma scopens (Hy. deteritum, Hyalomma asiaticum, Hyalomma marginatum, and Hyalomma anatolicum belonged to the genus Hyalomma. The frequency rates of Hyalomma, Dermacentor, Rhipicephalus, and Hemaphysalis genera were 73.74%, 15.05%, 10.03%, and 1.15 %, respectively. The highest abundance of ticks was observed in spring (152. Conclusion: The results of this study indicate the diversity of hard ticks in the region and the highest abundance of ticks in spring. Considering the importance of ticks in disease transmission among humans and domestic ruminants, health authorities and respective organizations should take appropriate health measures to control and combat these external parasites.

  9. Removal of drone brood from Apis mellifera (Hymenoptera: Apidae) colonies to control Varroa destructor (Acari: Varroidae) and retain adult drones.

    Science.gov (United States)

    Wantuch, Holly A; Tarpy, David R

    2009-12-01

    The parasitic mite Varroa destructor Anderson & Trueman (Acari: Varroidae) has plagued European honey bees, Apis mellifera L. (Hymenoptera: Apidae), in the Americas since its introduction in the 1980s. For many years, these mites were sufficiently controlled using synthetic acaricides. Recently, however, beekeepers have experienced increased resistance by mites to chemical pesticides, which are also known to leave residues in hive products such as wax and honey. Thus there has been increased emphasis on nonchemical integrated pest management control tactics for Varroa. Because mites preferentially reproduce in drone brood (pupal males), we developed a treatment strategy focusing on salvaging parasitized drones while removing mites from them. We removed drone brood from colonies in which there was no acaricidal application and banked them in separate "drone-brood receiving" colonies treated with pesticides to kill mites emerging with drones. We tested 20 colonies divided into three groups: 1) negative control (no mite treatment), 2) positive control (treatment with acaricides), and 3) drone-brood removal and placement into drone-brood receiving colonies. We found that drone-brood trapping significantly lowered mite numbers during the early months of the season, eliminating the need for additional control measures in the spring. However, mite levels in the drone-brood removal group increased later in the summer, suggesting that this benefit does not persist throughout the entire season. Our results suggest that this method of drone-brood trapping can be used as an element of an integrated control strategy to control varroa mites, eliminating a large portion of the Varroa population with limited chemical treatments while retaining the benefits of maintaining adult drones in the population.

  10. A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile.

    Science.gov (United States)

    Ogrzewalska, Maria; Nieri-Bastos, Fernanda A; Marcili, Arlei; Nava, Santiago; González-Acuña, Daniel; Muñoz-Leal, Sebastián; Ruiz-Arrondo, Ignacio; Venzal, José M; Mangold, Atilio; Labruna, Marcelo B

    2016-04-01

    The tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009-2013, and tested by PCR for rickettsial infection in the laboratory, and isolation of rickettsiae in Vero cell culture by the shell vial technique. Overall, 51 (62.2%) out of 82 A. parvitarsum adult ticks were infected by spotted fever group (SFG) rickettsiae, which generated DNA sequences 100% identical to each other, and when submitted to BLAST analysis, they were 99.3% identical to corresponding sequence of the ompA gene of Rickettsia sp. strain Atlantic rainforest. Rickettsiae were successfully isolated in Vero cell culture from two ticks, one from Argentina and one from Chile. DNA extracted from the third passage of the isolates of Argentina and Chile were processed by PCR, resulting in partial sequences for three rickettsial genes (gltA, ompB, ompA). These sequences were concatenated and aligned with rickettsial corresponding sequences available in GenBank. Phylogenetic analysis revealed that the A. pavitarsum rickettsial agent grouped under high bootstrap support in a clade composed by the SFG pathogens R. sibirica, R. africae, R. parkeri, Rickettsia sp. strain Atlantic rainforest, and two unnamed SFG agents of unknown pathogenicty, Rickettsia sp. strain NOD, and Rickettsia sp. strain ApPR. The pathogenic role of this A. parvitarsum rickettsia cannot be discarded, since several species of tick-borne rickettsiae that were considered nonpathogenic for decades are now associated with human infections. Copyright © 2016. Published by Elsevier GmbH.

  11. Phylogeny and species delineation in European species of the genus Steganacarus (Acari, Oribatida) using mitochondrial and nuclear markers.

    Science.gov (United States)

    Kreipe, Victoria; Corral-Hernández, Elena; Scheu, Stefan; Schaefer, Ina; Maraun, Mark

    2015-06-01

    Species of the genus Steganacarus are soil-living oribatid mites (Acari, Phthiracaridae) with a ptychoid body. The phylogeny and species status of the species of Steganacarus are not resolved, some authors group all ten German species of Steganacarus within the genus Steganacarus whereas others split them into three subgenera, Steganacarus, Tropacarus and Atropacarus. Additionally, two species, S. magnus and T. carinatus, comprise morphotypes of questionable species status. We investigated the phylogeny and species status of ten European Steganacarus species, i.e. S. applicatus, S. herculeanus, S. magnus forma magna, S. magnus forma anomala, S. spinosus, Tropacarus brevipilus, T. carinatus forma carinata, T. carinatus forma pulcherrima, Atropacarus striculus and Rhacaplacarus ortizi. We used two molecular markers, a 251 bp fragment of the nuclear gene 28S rDNA (D3) and a 477 bp fragment of the mitochondrial COI region. The phylogeny based on a combined analysis of D3 and COI separated four subgenera (Steganacarus, Tropacarus and Atropacarus, Rhacaplacarus) indicating that they form monophyletic groups. The COI region separated all ten species of the genus Steganacarus and showed variation within some species often correlating with the geographic origin of the species. Resolution of the more conserved D3 region was limited, indicating that radiation events are rather recent. Overall, our results indicate that both genes alone cannot be used for phylogeny and barcoding since variation is too low in D3 and too high in COI. However, when used in combination these genes provide reliable insight into the phylogeny, radiation and species status of taxa of the genus Steganacarus.

  12. Detection of Babesia spp. in Dogs and Their Ticks From Peninsular Malaysia: Emphasis on Babesia gibsoni and Babesia vogeli Infections in Rhipicephalus sanguineus sensu lato (Acari: Ixodidae).

    Science.gov (United States)

    Prakash, Batah Kunalan; Low, Van Lun; Vinnie-Siow, Wei Yin; Tan, Tiong Kai; Lim, Yvonne Ai-Lian; Morvarid, Akhavan Rezaei; AbuBakar, Sazaly; Sofian-Azirun, Mohd

    2018-05-12

    Canine babesiosis is an emerging tick-borne disease with a worldwide distribution, including Malaysia. While the prevalence of Babesia has been documented from dogs in Malaysia, occurrence of Babesia has been relatively little studied in their tick vectors. Accordingly, a total of 240 dogs and 140 Rhipicephalus sanguineus sensu lato (s.l.) (Acari: Ixodidae) ticks from Malaysia were molecularly screened for the presence of Babesia protozoa in the present study. Babesia gibsoni was only detected in ticks (1.4%), whereas Babesia vogeli was detected in both ticks (1.4%) and dogs (2.1%). This study highlights the detection of B. gibsoni and B. vogeli for the first time, in both adult and nymphal stages of R. sanguineus s.l. in Malaysia, suggesting the potential role of this tick species in transmitting canine babesiosis.

  13. GALEN skin test study III: Minimum battery of test inhalent allergens needed in epidemiological studies in patients

    DEFF Research Database (Denmark)

    Bousquet, P-J; Burbach, G; Heinzerling, L M

    2009-01-01

    Background: The number of allergens to be tested in order to identify sensitized patients is important in order to have the most cost-effective approach in epidemiological studies. Objective: To define the minimal number and the type of skin prick test (SPT) allergens required to identify a patient...... the allergens selection. Result: Among the 3034 patients involved, 1996 (68.2%) were sensitized to at least one allergen. Overall, eight allergens (grass pollen, Dermatophagoides pteronyssinus, birch pollen, cat dander, Artemisia, olive pollen, Blatella and Alternaria) allowed to identified more than 95......% of sensitized subjects. However, differences were observed between countries, two allergens being sufficient for Switzerland (grass pollen and cat dander) as opposed to nine for France (grass pollen, Dermatophagoides pteronyssinus, olive pollen, cat dander, Blatella, cypress, dog dander, alder and [Artemisia...

  14. Expression analysis of Drosophila doublesex, transformer-2, intersex, fruitless-like, and vitellogenin homologs in the parahaploid predator Metaseiulus occidentalis (Chelicerata: Acari: Phytoseiidae).

    Science.gov (United States)

    Pomerantz, Aaron F; Hoy, Marjorie A

    2015-01-01

    Characterization and expression analyses are essential to gain insight into sex-determination pathways in members of the Acari. Little is known about sex determination at the molecular level in the western orchard predatory mite Metaseiulus occidentalis (Arthropoda: Chelicerata: Arachnida: Acari: Phytoseiidae), a parahaploid species. In this study, eight genes previously identified as putative homologs to genes involved in the sex-determination pathway in Drosophila melanogaster were evaluated for sex-specific alternative splicing and sex-biased expression using reverse-transcriptase PCR and quantitative real-time PCR techniques, respectively. The homologs evaluated in M. occidentalis included two doublesex-like genes (Moccdsx1 and Moccdsx2), transformer-2 (Mocctra-2), intersex (Moccix), two fruitless-like genes (MoccBTB1 and MoccBTB2), as well as two vitellogenin-like genes (Moccvg1 and Moccvg2). Single transcripts of equal size were detected in males and females for Moccdsx1, Moccdsx2, Mocctra-2, Moccix, and MoccBTB2, suggesting that their pre-mRNAs do not undergo alternative splicing in a sex-specific manner. Three genes, Moccdsx1, Moccdsx2 and MoccBTB2, displayed male-biased expression relative to females. One gene, Moccix, displayed female-biased expression relative to males. Two genes, Mocctra-2 and MoccBTB1, did not display detectable differences in transcript abundance in males and females. Expression of Moccvg1 and Moccvg2 were detected in females only, and transcript levels were up-regulated in mated females relative to unmated females. To our knowledge, this represents the first attempt to elucidate expression patterns of putative sex-determination genes in an acarine. This study is an initial step towards understanding the sex-determination pathway in the parahaploid M. occidentalis.

  15. Does IFN-γ play a role on the pathogenesis of non-atopic asthma in Latin America children?

    OpenAIRE

    Figueiredo Camila Alexandrina; Rodrigues Laura Cunha; Alcantara-Neves Neuza Maria; Cooper Philip J; Amorim Leila Denise; Silva Nivea Bispo; Cruz Alvaro A; Barreto Mauricio Lima

    2012-01-01

    Abstract In this work we explore differences in blood cells and cytokine profiles in children according to atopic status and asthma (atopic or non-atopic). The study involved measurement of Th1(IFN-γ) and Th2 (IL-5 and IL-13) cytokines in Dermatophagoides pteronyssinus stimulated peripheral blood leukocytes, blood cell count, skin prick test and specific IgE against common aeroallergens. Atopic status was associated with eosinophilia and production of Th2 type cytokines. Atopic asthma was a...

  16. Novel bacterial pathogen Acaricomes phytoseiuli causes severe disease symptoms and histopathological changes in the predatory mite Phytoseiulus persimilis (Acari, Phytoseiidae).

    Science.gov (United States)

    Schütte, Conny; Gols, Rieta; Kleespies, Regina G; Poitevin, Olivier; Dicke, Marcel

    2008-06-01

    Adult female Phytoseiulus persimilis Athias-Henriot (Acari, Phytoseiidae) of a laboratory population show a set of characteristic symptoms, designated as non-responding (NR) syndrome. Mature predators shrink, cease oviposition and die. They show a lower degree of attraction to herbivore-induced plant volatiles and a greater tendency to leave prey patches carrying ample prey. Moreover, predators may carry excretory crystals in the legs, may cease prey consumption and have a low excretion rate. Here, we satisfy Koch's postulates for a strain of Acaricomes phytoseiuli (DSM 14247) that was isolated from symptomatic female P. persimilis of the NR-population. Adult female P. persimilis were either exposed to a bacterial inoculum suspension (treatment) or to sterile distilled water (control) for a period of 3 days. Control and treated predators were examined for the occurrence of six symptoms characteristic for the NR-syndrome and the presence of A. phytoseiuli after inoculation. The latter was done by re-isolation of A. phytoseiuli from individual predators and predator feces placed on nutrient agar, by PCR-based identification and by histopathological studies of individual predators. The NR-syndrome was clearly induced in those predators that had been exposed to the bacterial inoculum (incubation time=2-5 days, fraction shrunken females=80%), whereas predators exposed to water did not show the NR-syndrome. A. phytoseiuli was never isolated from control predators whereas it could be re-isolated from 60% of the treated predators (N=37) and from feces of 41% of treated predators (N=17). Only one day after exposure A. phytoseiuli could not be re-isolated from treated predators and their feces. Light and electron microscope studies of predators exposed to A. phytoseiuli revealed striking bacterial accumulations in the lumen of the alimentary tract together with extreme degeneration of its epithelium. In addition, bacterial foci also occurred in the fat body. These phenomena

  17. A novel disease affecting the predatory mite Phytoseiulus persimilis (Acari, Phytoseiidae): 2. Disease transmission by adult females.

    Science.gov (United States)

    Schütte, Conny; Poitevin, Olivier; Negash, Tesfaye; Dicke, Marcel

    2006-01-01

    Adult female Phytoseiulus persimilis Athias-Henriot (Acari, Phytoseiidae) of one of our laboratory populations (=NR-population), show the following set of symptoms: predators shrink several days after mating, cease egg production and die several days after shrinking, show a lower degree of attraction to herbivore-induced plant volatiles and a shorter choice time in olfactometer tests, have the tendency to leave a prey patch with ample food, may carry excretory crystals in the legs, may cease prey consumption, and have a lower excretion rate. We hypothesized earlier that this characteristic syndrome, called non-responding (=NR-) syndrome, is caused by a pathogen infecting P. persimilis. To further support this hypothesis we here study several transmission modes of the factor causing the NR-syndrome. In all tests we measured size, short-term fecundity, mortality, predator position, response to plant odors and crystal location, thus including 6 of the 9 symptoms known yet. No evidence was found for vertical transmission from parent to offspring. Eggs from symptomatic females of the NR-population mated by males of the NR-population gave rise to normal-sized, well performing predators, when they had been surface sterilized or transferred to a new leaf. However, such eggs gave rise to shrunken females (17%) when left on the leaf where they had been laid. In the latter case transmission via products deposited on the leaf by the mothers was possible. We therefore tested several modes of horizontal transmission by exposing females of a commercial population that never showed the NR-syndrome (=R1-population) to products related to the symptomatic NR-population. No evidence was found for transmission via food or via squashed adult females. However, symptoms were induced in adult females of the R1-population after a 3-day exposure to a live adult female of the NR-population (incubation period=3-7 days, fraction shrunken females=53%) and after a 1-day exposure to feces and

  18. Acaricidal activity of Cymbopogon citratus and Azadirachta indica against house dust mites.

    Science.gov (United States)

    Hanifah, Azima Laili; Awang, Siti Hazar; Ming, Ho Tze; Abidin, Suhaili Zainal; Omar, Maizatul Hashima

    2011-10-01

    To examine the acaricidal effects of the essential oil of Cymbopogon citratus leaf extract (lemongrass) and ethanolic Azadirachta indica leaf extract (neem) against house dust mites Dermatophagoides farinae (D. farinae) and Dermatophagoides pteronyssinus (D. pteronyssinus). Twenty-five adults mites were placed onto treated filter paper that is soaked with plant extract and been tested at different concentrations (50.00%, 25.00%, 12.50%, 6.25% and 3.13%) and exposure times (24hrs, 48hrs, 72hrs and 96 hrs). All treatments were replicated 7 times, and the experiment repeated once. The topical and contact activities of the two herbs were investigated. Mortalities from lemongrass extract were higher than neem for both topical and contact activities. At 50 % concentration, both 24 hrs topical and contact exposures to lemongrass resulted in more than 91% mortalities for both species of mites. At the same concentration and exposure time, neem resulted in topical mortalities of 40.3% and 15.7% against D. pteronyssinus and D. farinae respectively; contact mortalities were 8.0% and 8.9% against the 2 mites, respectively. There was no difference in topical mortalities of D. pteronyssinus from exposure to concentrations of lemongrass and neem up to 12.50%; lemongrass was more effective than neem at the higher concentrations. Generally, topical mortalities of D. farinae due to lemongrass are higher than that due to neem. Contact mortalities of lemongrass are always higher that neem against both species of mites.

  19. Ocorrência de Ornithonyssus bursa (Berlese, 1888 (Acari: Macronyssidae em filhotes de Megascops choliba (corujinha-do-mato e Pitangus sulphuratus (bem-te-vi, no Rio Grande do Sul, Brasil Ocurrence of Ornithonyssus bursa (Berlese, 1888 (Acari: Macronyssidae on Megascops choliba (tropical screech-owl and Pitangus sulphuratus (great kiskadee nestlings in the Rio Grande do Sul State, Brazil

    Directory of Open Access Journals (Sweden)

    Carolina S. Mascarenhas

    2009-12-01

    Full Text Available O Núcleo de Reabilitação da Fauna Silvestre e Centro de Triagem de Animais Silvestres da Universidade Federal de Pelotas - RS atendeu dois filhotes de Megascops choliba (corujinha-do-mato (Strigiformes - Strigidae e dois de Pitangus sulphuratus (bem-te-vi (Passeriformes - Tyrannidae intensamente parasitados por ácaros, em maio de 2005 e dezembro de 2006, respectivamente. Os filhotes e o ninho de P. sulphuratus foram recolhidos na zona urbana da cidade de Pelotas - RS após forte temporal. Os ácaros foram removidos, colocados em álcool 70% e levados ao laboratório de parasitologia para identificação. Os espécimes foram clarificados em lactofenol, montados em meio de Hoyer e identificados como Ornithonyssus bursa (Acari - Macronyssidae. Registra-se Megascops choliba e Pitangus sulphuratus como hospedeiros de Ornithonyssus bursa, no Rio Grande do Sul, Brasil.The Center for Rehabilitation of Wildlife and Center for Selection of Wild Animal of the Federal University of Pelotas has attended two nestlings of Megascops choliba (tropical screech-owl (Strigiformes - Strigidae and two of Pitangus sulphuratus (great kiskadee (Passeriformes - Tyrannidae heavily parasitized by mites, in May 2005 and December 2006, respectively. The nestlings and the nest of P. sulphuratus were collected in the Pelotas urban area after severe storms. The mites were removed, clarified in lactofenol, permanently mounted in Hoyer's medium and identified as Ornithonyssus bursa (Acari - Macronyssidae. Megascops choliba and Pitangus sulphuratus are reported as host of Ornithonyssus bursa in Rio Grande do Sul State, Brazil.

  20. Biological cycle of Tenuipalpus heveae Baker (Acari, Tenuipalpidae on leaflets of three rubber tree clones Ciclo de vida de Tenuipalpus heveae Baker (Acari, Tenuipalpidae em folíolos de três clones de seringueira

    Directory of Open Access Journals (Sweden)

    Reinaldo José Fazzio Feres

    2010-06-01

    Full Text Available Life cycle of Tenuipalpus heveae Baker (Acari, Tenuipalpidae on leaflets from three rubber tree clones. The biological cycle of Tenuipalpus heveae Baker, 1945 (Tenuipalpidae, a potential rubber tree pest mite, was studied by the observation of individuals reared on leaflets of the clones GT 1, PB 235 and RRIM 600, in controlled environmental conditions. Three daily observations were done of 60 eggs on leaflets from each clone in order to verify the development of immature stages and the female oviposition. The fertility life table was constructed based in the collected data. Mites reared on PB 235 had faster rate of development, requiring less time in days, to double its population in number (TD, and had the highest values for egg production, female longevity, net reproductive rate (Ro, intrinsic rate of natural increase (r m and finite rate of increase (λ. Lower reproductive values and the longest time necessary to reach adult stage were recorded for the mites on GT 1. In all studied clones, the deutonymphal phase had the highest viability, while the larval phase had the lowest, highlighted by the survivorship curve that indicated high mortality during this life stage. The clone PB 235 allowed the most suitable conditions for the development of T. heveae, followed by RRIM 600, while GT 1 was the less suitable substratum to rear this mite species.O ciclo de vida de Tenuipalpus heveae Baker, 1945 (Tenuipalpidae, um potencial ácaro-praga da seringueira, foi estudado a partir de indivíduos criados sobre folíolos destacados dos clones GT 1, PB 235 e RRIM 600, em condições controladas. Três observações diárias foram realizadas, acompanhando-se o desenvolvimento de 60 ovos e de sua prole em folíolos de cada um dos clones, para verificação da oviposição das fêmeas e dos estágios de desenvolvimento. A partir dos dados obtidos, foi elaborada uma tabela de vida de fertilidade. Os ácaros criados sobre folíolos de PB 235 apresentaram r

  1. Characterization of the complete mitochondrial genome of the storage mite pest Tyrophagus longior (Gervais) (Acari: Acaridae) and comparative mitogenomic analysis of four acarid mites.

    Science.gov (United States)

    Yang, Banghe; Li, Chaopin

    2016-02-01

    Mites of the genus Tyrophagus are economically important polyphagous pest commonly living on stored products and also responsible for allergic reactions to humans. Complete mitochondrial genomes (mitogenomes) and the gene features therein are widely used as molecular markers in the study of population genetics, phylogenetics as well as molecular evolution. However, scarcity on the sequence data has greatly impeded the studies in these areas pertaining to the Acari (mites and ticks). Information on the Tyrophagus mitogenomes is quite critical for phylogenetic evaluation and molecular evolution of the mitogenomes within Acariformes. Herein, we reported the complete mitogenome of the allergenic acarid storage mite Tyrophagus longior (Astigmata: Acaridae), an important member of stored food pests, and compared with those of other three acarid mites. The complete mitogenome of T. longior was a circular molecule of 13,271 bp. Unexpectedly, only 19 transfer RNA genes (tRNAs) were present, lacking trnF, trnS1 and trnQ. Furthermore, it also contained 13 protein-coding genes (PCGs) and 2 genes for rRNA (rrnS and rrnL) commonly detected in metazoans. The four mitogenomes displayed similar characteristics with respect to the gene content, nucleotide comparison, and codon usages. Yet, the gene order of T. longior was different from that in other Acari. The J-strands of the four mitogenomes possessed high A+T content (67.4-70.0%), and exhibited positive GC-skews and negative AT-skews. Most inferred tRNAs of T. longior were extremely truncated, lacking either a D- or T-arm, as found in other acarid mites. In T. longior mitogenome the A+T-rich region was just 50 bp in length and can be folded as a stable stem-loop structure, whereas in the region some structures of microsatellite-like (AT)n and palindromic sequences was not present. Besides, reconstructing of the phylogenetic relationship based on concatenated amino acid sequences of 13 PCGs supported that monophyly of the family

  2. Diversity of Quill Mites of the Family Syringophilidae (Acari: Prostigmata) Parasitizing Owls (Aves: Strigiformes) With Remarks on the Host-Parasite Relationships.

    Science.gov (United States)

    Skoracki, Maciej; Unsoeld, Markus; Marciniak, Natalia; Sikora, Bozena

    2016-07-01

    The quill mite fauna of the family Syringophilidae (Acari: Prostigmata: Cheyletoidea) associated with owls (Aves: Strigiformes) is reviewed. A new genus is proposed, Neobubophilus Skoracki & Unsoeld gen. nov. It differs from closely related Bubophilus (Bubophilus Philips and Norton, 1978) by the absence of leg setae vsII in the both sexes. In addition, four new species are described: (1) Neobubophilus cunicularius Skoracki & Unsoeld sp. nov. from Athene cunicularia (Molina, 1782) (Strigidae) from Paraguay; (2) Neobubophilus atheneus Skoracki & Unsoeld sp. nov. from Athene noctua (Scopoli, 1769) and Athene brama (Temminck, 1821) (Strigidae), both from India; (3) Bubophilus tytonus Skoracki & Unsoeld sp. nov. from Tyto alba affinis (Blyth, 1862) (Tytonidae) from Cameroon, and (4) Megasyringophilus dalmas Skoracki & Unsoeld sp. nov. from Megascops choliba (Vieillot, 1817) (Strigidae) from Venezuela. The following new host species are given: Bubo bubo (Linnaeus, 1758) (Strigidae) from Nepal for Bubophilus ascalaphus (Philips and Norton 1978) and Strix woodfordii (Smith, 1834) (Strigidae) from Tanzania for Bubophilus aluconis (aluconis Nattress and Skoracki 2009). A key for syringophilid genera and species associated with owls is constructed. The host-parasite relationships of syringophilid mites and owls are discussed. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com Version of Record, first published online May 24, 2016 with fixed content and layout in compliance with Art. 8.1.3.2 ICZN.

  3. Toxicological evaluation of genetically modified cotton (Bollgard) and Dipel WP on the non-target soil mite Scheloribates praeincisus (Acari: Oribatida).

    Science.gov (United States)

    Oliveira, Anibal R; Castro, Thiago R; Capalbo, Deise M F; Delalibera, Italo

    2007-01-01

    Insecticides derived from the bacterium Bacillus thuringiensis (Bt) and plants genetically modified (GM) to express B. thuringiensis toxins are important alternatives for insect pest control worldwide. Risk assessment of B. thuringiensis toxins to non-target organisms has been extensively studied but few toxicological tests have considered soil invertebrates. Oribatid mites are one of the most diverse and abundant arthropod groups in the upper layers of soil and litter in natural and agricultural systems. These mites are exposed to the toxic compounds of GM crops or pesticides mainly when they feed on vegetal products incorporated in the soil. Although some effects of B. thuringiensis products on Acari have been reported, effects on oribatid mites are still unknown. This study investigated the effects of the ingestion of Bt cotton Bollgard and of the B. thuringiensis commercial product Dipel WP on the pantropical species Scheloribates praeincisus (Scheloribatidae). Ingestion of Bollgard and Dipel did not affect adult and immature survivorship and food consumption (estimated by number of fecal pellets produced daily) or developmental time of immature stages of S. praeincisus. These results indicate the safety of Bollgard and Dipel to S. praeincisus under field conditions where exposition is lower and other food sources besides leaves of Bt plants are available. The method for toxicological tests described here can be adapted to other species of Oribatida, consisting on a new option to risk assessment studies.

  4. Dinâmica populacional de Calepitrimerus vitis (Nalepa (Acari: eriophyidae em cultivares de videira na região da campanha do Rio Grande do Sul

    Directory of Open Access Journals (Sweden)

    Paulo Ricardo Ebert Siqueira

    2013-06-01

    Full Text Available Na Região da Campanha do Rio Grande do Sul, o ácaro-da-ferrugem-da-videira, Calepitrimerus vitis (Nalepa (Acari: Eriophyidae, é encontrado com frequência em vinhedos de cultivares europeias, desde a safra de 2004/2005, causando bronzeamento nas folhas. A dinâmica populacional de C. vitis nas cultivares Chardonnay e Merlot foi avaliada em vinhedo comercial localizado no município de Dom Pedrito, na região da Campanha, durante os anos agrícolas de 2005/2006 e 2006/2007, por meio de amostragem realizada em folhas das posições basal, intermediária e apical de ramos de produção. O pico populacional de C. vitis ocorre entre o final de fevereiro e o início de março, sendo seguido de forte declínio populacional. A infestação variou de intensidade entre as cultivares de acordo com o ano, sendo a cultivar Chardonnay mais infestada no primeiro ano, e Merlot, no segundo. Folhas na posição basal, mediana e apical apresentam níveis similares de infestação. Uma correlação positiva foi encontrada entre o número de C. vitis na face abaxial das folhas e o percentual de folhas com infestação.

  5. Toxicidade diferencial de produtos à base de abamectina ao ácaro Brevipalpus phoenicis (Geijskes, 1939 (Acari: Tenuipalpidae em citros Differential toxicity of abamectin based products over Brevipalpus phoenicis (Geijskes, 1939 (Acari: Tenuipalpidae mite in citrus

    Directory of Open Access Journals (Sweden)

    Daniel Júnior de Andrade

    2010-03-01

    Full Text Available O ácaro Brevipalpus phoenicis é uma das principais pragas dos citros por ser vetor do "Citrus Leprosis Virus" (CiLV, agente causal da leprose, uma das mais graves doenças da citricultura. Objetivou-se avaliar o efeito tóxico de produtos à base de abamectina sobre o ácaro B. phoenicis. Foram realizados um experimento de ação direta e três de ação residual no Laboratório de Acarologia do Departamento de Proteção de Plantas (Fitossanidade da FCAV - UNESP, Jaboticabal-SP. O delineamento adotado nos bioensaios foi o inteiramente casualizado, onde 10 tratamentos foram repetidos 7 vezes, sendo cada repetição composta por um fruto de laranja. Os tratamentos estudados (mL p.c./100 L de água foram: Acaramik a 20; 30; 40 e 50 mL; Vertimec a 30 e 40 mL; Abamectin Nortox a 30 e 40 mL; Tricofol a 77 mL e uma testemunha sem aplicação. Utilizaram-se frutos com presença de verrugose, que foram lavados e parcialmente parafinados, deixando-se uma área sem parafina, que foi circundada com cola entomológica para contenção dos ácaros. Transferiram-se 20 ácaros adultos B. phoenicis para cada fruto. No bioensaio de ação direta, a transferência foi realizada antes das aplicações e, nos bioensaios de ação residual, aos 5; 10 e 15 dias após a aplicação dos produtos. A aplicação dos produtos sobre os frutos foi realizada em Torre de Potter. Os resultados obtidos nos bioensaios evidenciaram que os melhores tratamentos foram: Tricofol a 77 mL, Acaramik a 40 e 50 mL e Vertimec a 40 mL. De forma geral, os produtos testados podem ser utilizados no controle do ácaro B. phoenicis.The mite Brevipalpus phoenicis (Geijskes, 1939 (Acari: Tenuipalpidae is one of the most important pests in Brazil citrus plantation, because it is the virus "Citrus Leprosis Virus" (CiLV vector, one of the most serious citrus plantation diseases. The purpose of this experiment was to evaluate the toxical effect of abamectin in the mite B. phoenicis. It was performed

  6. Elenco y biogeografía de los ácaros acuáticos (Acari, Parasitengona, Hydrachnidia de Sudamérica

    Directory of Open Access Journals (Sweden)

    Rosso de Ferradás, B.

    2005-12-01

    Full Text Available Checklist and catalog publications demonstrate a clear connection between basic taxonomy and biodiversity issues. According to some estimates, only 10-30% of all global species have been named. As in other fields, catalogs provide an important source of information concerning species diversity in freshwater ecology.

    South America is a continent dominated by freshwater ecosystems. The tremendous habitat diversity created by this landscape supports a high number of arthropods, including water mites, which belong to the hyperdiverse group Acari. South America has a substantial task ahead in cataloging its biodiversity.

    Much has been published on water mites in South America. In fact, according to Besch, water mites were the most studied with the exception of Europe up until the 1960’s. Most of the collections were conducted by two acarologists (Lundblad and Karl Viets during the 1940’s. Today, the collection, identification and description process of water mites is slower. In the 1980’s, the north-american acarologist D. R. Cook produced two lengthy papers about neotropical water mites in four regions of South America. Recently, several Argentine acarologists have published papers on water mites from diverse habitats and regions in South America.

    The catalog presented here includes information regarding 6 superfamilies, including 23 families in 118 genera of true water mites (Hydrachnidia, Parasitengona, Acari. It also includes the references concerning the species, distribution in various regions of South America and –as far as known– habitat.

    At present, there are 916 species from several authors cataloged in 11 countries in South America. The degree of knowledge varies greatly from country to country, with numerous entries for Brazil and none for French Guiana.

    Las publicaciones de catálogos y listados de especies determinan una clara conexión entre taxonomía básica y temas

  7. Detection of B-lymphocytes secreting antibodies to Dermatophagoides antigens

    DEFF Research Database (Denmark)

    Sparholt, S H; Barington, T; Schou, C

    1991-01-01

    Alutard, showed that 1 week after injection, circulating allergen-specific antibody-secreting cells (AbSC) were detected with this assay. The ranges were between 1 and 13 IgM-, 2 and 20 IgG- and 20 and 55 IgA allergen-specific AbSC per 10(6) MNC. It is expected that this assay will help to understand more...

  8. Long-Term Effects of Berberis thunbergii (Ranunculales: Berberidaceae) Management on Ixodes scapularis (Acari: Ixodidae) Abundance and Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) Prevalence in Connecticut, USA.

    Science.gov (United States)

    Williams, Scott C; Linske, Megan A; Ward, Jeffrey S

    2017-12-08

    Japanese barberry (Berberis thunbergii de Candolle; Ranunculales: Berberidaceae) is an exotic invasive shrub that escaped cultivation in the United States and is now permanently established in many eastern and midwestern states. This study examined the long-term impacts of Japanese barberry management on blacklegged tick (Ixodes scapularis Say; Acari: Ixodidae) abundances and associated prevalence of Borrelia burgdorferi (Johnson, Schmid, Hyde, Steigerwalt, and Brenner; Spirochaetales: Spirochaetaceae), the etiologic agent of Lyme disease. At six locations across Connecticut, adult I. scapularis were sampled for up to 10 yr. At each location, we sampled an area where barberry infestations were unmanipulated, adjacent areas where barberry was virtually nonexistent, and areas where barberry was managed utilizing a variety of techniques. Barberry management reduced B. burgdorferi-infected adult I. scapularis (BBIAIS) abundances (191/ha ± 64 SE) over 6 yr to statistically indifferent from that of no barberry areas (140/ha ± 47 SE; P = 0.080) and significantly less than intact barberry stands (458/ha ± 80 SE; P = 0.026). Over 9 yr, BBIAIS abundances in managed barberry remained lower than intact barberry stands (P = 0.037), but increased to be significantly greater than no barberry areas (P = 0.007) as cover increased over time. Longer-term data further document that Japanese barberry infestations are favorable habitat for I. scapularis. Control of Japanese barberry and other invasives should be at least on a 5-yr rotation to maintain low levels of invasive cover and eliminate humidity refugia to expose juvenile I. scapularis to more hostile environmental conditions in the interest of public health. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  9. Ability of two natural products, nootkatone and carvacrol, to suppress Ixodes scapularis and Amblyomma americanum (Acari: Ixodidae) in a Lyme disease endemic area of New Jersey.

    Science.gov (United States)

    Dolan, Marc C; Jordan, Robert A; Schulze, Terry L; Schulze, Christopher J; Manning, Mark Cornell; Ruffolo, Daniel; Schmidt, Jason P; Piesman, Joseph; Karchesy, Joseph J

    2009-12-01

    We evaluated the ability of the natural, plant-derived acaricides nootkatone and carvacrol to suppress Ixodes scapularis Say and Amblyomma americanum (L.) (Acari: Ixodidae). Aqueous formulations of 1 and 5% nootkatone applied by backpack sprayer to the forest litter layer completely suppressed I. scapularis nymphs through 2 d. Thereafter, the level of reduction gradually declined to nootkatone was less effective, but at a 5% concentration, the level of control was similar or greater to that observed with I. scapularis through 21 d postapplication. Initial applications of 0.05% carvacrol were ineffective, but a 5% carvacrol formulation completely suppressed nymphs of both species through 2 d and resulted in significant reduction in I. scapularis and A. americanum nymphs through 28 and 14 d postapplication, respectively. Backpack sprayer applications of 5% nootkatone to the shrub and litter layers resulted in 100% control of I. scapularis adults through 6 d, but the level of reduction declined to 71.5% at 28 d postapplication. By contrast, high-pressure applications of 2% nootkatone to the litter layer resulted in 96.2-100% suppression of both I. scapularis and A. americanum nymphs through 42 d, whereas much lower control was obtained from the same formulation applied by backpack sprayer. Backpack sprayer application of a 3.1% nootkatone nanoemulsion resulted in 97.5-98.9 and 99.3-100% reduction in I. scapularis and A. americanum nymphs, respectively, at 1 d postapplication. Between 7 d and 35 d postapplication, the level of control varied between 57.1% and 92.5% for I. scapularis and between 78.5 and 97.1% for A. americanum nymphs. The ability of natural products to quickly suppress and maintain significant control of populations of these medically important ticks at relatively low concentrations may represent a future alternative to the use of conventional synthetic acaricides.

  10. Suppression of host-seeking Ixodes scapularis and Amblyomma americanum (Acari: Ixodidae) nymphs after dual applications of plant-derived acaricides in New Jersey.

    Science.gov (United States)

    Jordan, Robert A; Dolan, Marc C; Piesman, Joseph; Schulze, Terry L

    2011-04-01

    We evaluated the ability of dual applications of natural, plant-derived acaricides to suppress nymphal Ixodes scapularis Say and Amblyomma americanum (L.) (Acari: Ixodidae) in a Lyme disease endemic area of New Jersey. An aqueous formulation of 2% nootkatone provided >90% control of I. scapularis through 7 d. Control declined to 80.9% at 14 d, and a second application was made that provided >95% control through the remaining 4 wk of the nymphal season. Nootkatone provided >90% control of A. americanum through 35 d postapplication. Applications of 2% carvacrol and EcoTrol T&O resulted in rapid knockdown of both tick species, but control declined significantly to 76.7 and 73.7%, respectively, after 14 d when a second application was made that extended control of both tick species to between 86.2 and 94.8% at 21 d. Subsequently, control declined steadily in all plots by 42 d postapplication except for I. scapularis in carvacrol-treated plots, where levels of control >90% were observed through 35 d. Of the three compounds tested, 2% nootkatone provided the most consistent results, with 96.5 and 91.9% control of I. scapularis and A. americanum through 42 and 35 d, respectively. The ability of plant-derived natural products to quickly suppress and maintain significant control of populations of these medically important ticks may represent a future alternative to the use of conventional synthetic acaricides. In addition, the demonstrated efficacy of properly-timed backpack sprayer application may enable homeowner access to these minimal-risk acaricides.

  11. A review of the ticks (Acari, Ixodida of Brazil, their hosts and geographic distribution - 1. The State of Rio Grande do Sul, Southern Brazil

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    Evans DE

    2000-01-01

    Full Text Available A review of the ticks (Acari, Ixodida of the State of Rio Grande do Sul, southern Brazil, was completed as a step towards a definitive list (currently indicated as 12 of such species, their hosts and distribution. The ticks: Argas miniatus (poultry, Ixodes loricatus (opossums, Amblyomma aureolatum (dogs, A. calcaratum (anteaters, A. cooperi (capybaras, A. nodosum (anteaters, A. tigrinum (dogs (Neotropical and Rhipicephalus sanguineus (dogs (introduced, cosmopolitan, Afrotropical were confirmed as present, in addition to the predominant, Boophilus microplus (cattle (introduced, pan-tropical, Oriental. Of the further 18 species thus far reported in the literature as present in the state, but unavailable for examination: only Ornithodoros brasiliensis (humans and their habitations (Neotropical, Ixodes affinis (deer (Nearctic/Neotropical and I. auritulus (birds (Nearctic/Neotropical/Afrotropical/ Australasian are considered likely; 13 species would benefit from corroborative local data but the majority appear unlikely; reports of A. maculatum (Nearctic/Neotropical, but circum-Caribbean are considered erroneous; the validity of A. fuscum is in doubt. The very recent, first known report of the tropical Anocentor nitens (horses(Nearctic/Neotropical, but still apparent absence of the tropical A. cajennense (catholic (Nearctic/Neotropical and the sub-tropical/temperate Ixodes pararicinus (cattle (Neotropical in Rio Grande do Sul are important for considerations on their current biogeographical distribution and its dynamics in South America. The state has relatively long established, introduced ("exotic", Old World tick species (B. microplus, R. sanguineus that continue to represent significant pests and disease vectors to their traditional, introduced domestic animal hosts, cattle and urban dogs. There are also indigenous, New World ticks (A. miniatus, O. brasiliensis, A. aureolatum, A. nitens, as both long established and possibly newly locally

  12. Chelicerata: Acari II

    NARCIS (Netherlands)

    Di Sabatino, A.; Gerecke, R.; Gledhill, T.; Smit, H.

    2010-01-01

    Chelicerata are a basically terrestrial group of invertebrates, including many clades whose representatives have never found an evolutionary way to aquatic live. An exception is made by some spiders and the highly diverse aquatic mites which in inland water habitats are represented by members of

  13. Effects of powdery mildew fungicide programs on twospotted spider mite (Acari: Tetranychidae), hop aphid (Hemiptera: Aphididae), and their natural enemies in hop yards.

    Science.gov (United States)

    Gent, D H; James, D G; Wright, L C; Brooks, D J; Barbour, J D; Dreves, A J; Fisher, G C; Walton, V M

    2009-02-01

    Twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), and hop aphid, Phorodon humuli (Schrank) (Hemiptera: Aphididae), are the most important arthropod pests of hop (Humulus lupulus L.) in the Northern Hemisphere. A potential barrier for greater adoption of conservation biological control strategies for spider mites and hop aphid is the extensive use of fungicides for management of hop powdery mildew, Podosphaera macularis (Wallr.:Fr.) U. Braun & S. Takamatsu. Field studies conducted in experimental plots in Oregon and Washington in 2005 and 2006 quantified the effects of powdery mildew fungicide programs (i.e., sulfur, paraffinic oil, and synthetic fungicides) on arthropod pests and natural enemies on hop. Fungicide treatment significantly affected spider mite populations in all four studies. Multiple applications of sulfur fungicides applied before burr development resulted in 1.4-3.3-fold greater spider mite populations during summer. Near the cessation of the sulfur applications, or after a lag of 20-30 d, spider mite populations increased significantly faster on sulfur treated plants compared with water-treated plants in three of four experiments. The effect of paraffinic oil on spider mites was varied, leading to exacerbation of spider mites in Oregon and Washington in 2005, suppression of mites in Oregon in 2006, and no significant effect compared with water in Washington in 2006. Significant relative treatment effects for cone damage due to spider mite feeding were detected in Oregon in 2005 in plots treated with sulfur and paraffinic oil compared with water and synthetic fungicides. Mean populations of hop aphids were similar among treatments in Oregon, although sulfur treatment suppressed hop aphid populations in Washington in 2005 and 2006. Populations of individual predacious insect species and cumulative abundance of macropredators were not consistently suppressed or stimulated by treatments in all trials. However, predatory mite

  14. The efficacy of E.P.D., a new immunotherapy, in the treatment of allergic diseases in children.

    Science.gov (United States)

    Caramia, G; Franceschini, F; Cimarelli, Z A; Ciucchi, M S; Gagliardini, R; Ruffini, E

    1996-11-01

    A double blind study was made on a group of 35 children, 8 of whom were allergic to Grass and 27 allergic to Pteronyssinus and Farinae Dermatophagoides. We verified the efficacy and tolerability of a new immunotherapy called E.P.D. (Enzyme Potentiated Desensitization). This particular immunotherapy consists in an intradermal injection of a mix made up of an allergic solution at extremely low doses and an enzyme, beta-glucuronidase. The vaccine is administered once a year, two weeks before pollen peaks for children with seasonal allergies and two times a year, in February and November, for children with non-seasonal allergies (Dermatophagoides). The results, statistically analyzed on the basis of a symptoms score, showed good clinical efficacy in patients affected by both seasonal and non-seasonal allergies. Due to the clinical effectiveness, easy administration and excellent tolerability of the immunotherapy, E.P.D. is particularly suited for treating or reducing allergic symptoms in allergic children.

  15. Atopy patch tests in young adult patients with atopic dermatitis and controls

    DEFF Research Database (Denmark)

    Bygum, Anette; Mørtz, Charlotte G; Andersen, Klaus Ejner

    2003-01-01

    -dependent results were obtained with Dermatophagoides pteronyssinus, grass and cat with a reproducibility rate of 0.69 to 0.81 in patients and 0.60-0.96 in controls. A unique finding was a significant positive correlation between a positive atopy patch test, allergen dose and increase in transepidermal water loss......The clinical interpretation and reproducibility of atopy patch tests was studied in 23 selected young adult patients with atopic dermatitis and 25 healthy controls using standard inhalant allergens. Non-invasive measurements were used for objective assessment of test reactions and the participants...

  16. Immunoinformatics and Similarity Analysis of House Dust Mite Tropomyosin

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    Mohammad Mehdi Ranjbar

    2015-10-01

    Full Text Available Background: Dermatophagoides farinae and Dermatophagoides pteronyssinus are house dust mites (HDM that they cause severe asthma and allergic symptoms. Tropomyosin protein plays an important role in mentioned immune and allergic reactions to HDMs. Here, tropomyosin protein from Dermatophagoides spp. was comprehensively screened in silico for its allergenicity, antigenicity and similarity/conservation.Materials and Methods: The amino acid sequences of D. farinae tropomyosin, D. pteronyssinus and other mites were retrieved. We included alignments and evaluated conserved/ variable regions along sequences, constructed their phylogenetic tree and estimated overall mean distances. Then, followed by with prediction of linear B-cell epitope based on different approaches, and besides in-silico evaluation of IgE epitopes allergenicity (by SVMc, IgE epitope, ARPs BLAST, MAST and hybrid method. Finally, comparative analysis of results by different approaches was made.Results: Alignment results revealed near complete identity between D. farina and D. pteronyssinus members, and also there was close similarity among Dermatophagoides spp. Most of the variations among mites' tropomyosin were approximately located at amino acids 23 to 80, 108 to 120, 142 to 153 and 220 to 230. Topology of tree showed close relationships among mites in tropomyosin protein sequence, although their sequences in D. farina, D. pteronyssinus and Psoroptes ovis are more similar to each other and clustered. Dermanyssus gallinae (AC: Q2WBI0 has less relationship to other mites, being located in a separate branch. Hydrophilicity and flexibility plots revealed that many parts of this protein have potential to be hydrophilic and flexible. Surface accessibility represented 7 different epitopes. Beta-turns in this protein are with high probability in the middle part and its two terminals. Kolaskar and Tongaonkar method analysis represented 11 immunogenic epitopes between amino acids 7-16. From

  17. Primera aproximacion a la Paleoentomología de los yacimientos de la Sierra de Atapuerca (Burgos, España: la fauna subfósil de Oribátidos (Acari, Oribatida

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    Arroyo, J.

    2007-06-01

    Full Text Available The Atapuerca Quaternarian sites are of worldwide interest due to the presence of human remains belonging to the last million years. The oribatid mites (Acari, Oribatida found in several archaeological samples extracted from the Atapuerca Quaternarian deposits, have been analyzed during the 2003 excavation campaign to study the Palaeoentomology of the site. The oribatid mite fauna consists of 7 individuals, 6 of which were obtained from Gran Dolina site (about 300.000 years old and belong to families Cosmochthoniidae, Scheloribatidae, Oribatulidae and Hemileiidae. Most of the taxa were identified to species level. The results obtained were used as a basis to reconstruct the paleo-environments of the site in correspondence with the biological and ecological preferences of the taxa.

    Los yacimientos cuaternarios de la Sierra de Atapuerca ofrecen interés mundial por haber hospedado varias especies de homínidos que vivieron en el último millón de años. En este contexto, durante la campaña de excavaciones de 2003 se realizó un muestreo puntual en algunos de sus yacimientos para obtener los primeros datos de restos de artrópodos que pudieran conservarse en los mismos. En este trabajo se presentan los resultados obtenidos de ácaros subfósiles pertenecientes al suborden de los oribátidos (Acari, Oribatida. Después de procesar los sedimentos, se obtuvieron un total de 7 individuos en el conjunto de los yacimientos muestreados, de los cuales 6 aparecieron en Gran Dolina en un nivel con datación en torno a los 300.000 años. Los ejemplares, identificados en su mayoría a nivel taxonómico de especie, pertenecen a las familias Cosmochthoniidae, Scheloribatidae, Oribatulidae y Hemileiidae. Dado el conocimiento que se dispone de la biología de los taxones encontrados, que pertenecen a géneros y especies presentes en la actualidad, se han realizado inferencias sobre los ambientes pretéritos en que los animales vivieron.

  18. House dust mites in the city of Lima, Peru.

    Science.gov (United States)

    Croce, M; Costa-Manso, E; Baggio, D; Croce, J

    2000-01-01

    Since mites are the most common house dust allergens, knowledge about the species most prevalent in a region is important for diagnostic and specific immunotherapy purposes. In order to establish the prevalence of house dust mites in different city districts, 100 house dust samples were collected from different parts of Lima. Lima is a city of tropical climate located along the coast of the Pacific Ocean. The relative air humidity is 80-90% and the various districts studied are located at altitudes ranging from 37-355 meters. The mite Blomia tropicalis was the organism most frequently detected, being present in 59% of the house dust samples. Dermatophagoides pteronyssinus occupied second place (15.9%), followed by Chortoglyphus arcuatus and Tyrophagus putrescentiae. These four mites, taken together, represented more than 90% of the mites detected. No specimen of the species Dermatophagoides farinae was detected. We conclude that B. tropicalis and D. pteronyssinus are the most common house dust mites in Lima. Considering the high prevalence of B. tropicalis in Lima and the fact that its cross-reactivity with antigens of the mites of the family Pyroglyphidae is minimal, we conclude that sensitization to this mite should be investigated separately in allergic patients living in Lima.

  19. Chlorotic spots on Clerodendrum, a disease caused by a nuclear type of Brevipalpus (Acari:Tenuipalpidae transmitted virus Mancha clorótica do Clerodendrum, uma enfermidade causada por um vírus do tipo nuclear, transmitido pelo ácaro Brevipalpus phoenicis (Acari:Tenuipalpidae

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    Elliot Watanabe Kitajima

    2008-02-01

    Full Text Available Chlorotic spots have been observed in plants of Clerodendrum x speciosum growing in residential gardens and parks in Piracicaba, SP, Brazil. Thin sections of diseased tissues revealed characteristic cytopathic effects of the nuclear type of the Brevipalpus (Acari: Tenuipalpidae mite-transmitted viruses (BTrV. Brevipalpus mites, identified as B. phoenicis, infesting symptomatic C. x speciosum plants transmitted the pathogen to healthy C. x speciosum and to C. thomsonae, Gomphrena globosa, Hibiscus cannabinus, H. coccineus, H. schizopetalus, Salvia leucantha, Spathiphyllum wallasi and Tetragonia expansa causing chlorotic spots on their leaves. Mechanical inoculation using leaf extracts from infected C. x speciosum resulted in chlorotic spots on inoculated C. x speciosum, Chenopodium quinoa, C. amaranticolor, G. globosa, H. cannabinus, H. coccineus and T. expansa leaves. C. amaranticolor and C. quinoa kept at 28 - 30°C became systemically infected. The same cytopathic effects caused by the nuclear type of BTrV were seen in tissues from all infected test plants by electron microscopy. The virus was purified from systemically infected leaves of C. amaranticolor and C. quinoa. A polyclonal antiserum obtained from an immunized rabbit presented a strong reaction with the homologous antigen in ELISA tests. The results suggest that this chlorotic spot disease of C. x speciosum is caused by a new species of the nuclear type of BTrV, tentatively named Clerodendrum chlorotic spot virus (ClCSV.Manchas cloróticas e necróticas foram observadas em folhas de várias plantas de coração-sangrento (Clerodendrum x speciosum cultivadas em parques e jardins em Piracicaba, SP, associadas à infestação pelo ácaro tenuipalpídeo Brevipalpus phoenicis. Exames preliminares de secções de tecido das manchas cloróticas ao microscópio eletrônico revelaram a ocorrência de efeitos citopáticos característicos dos induzidos pelos vírus do tipo nuclear, transmitido

  20. Influence de l'usage préventif des pesticides sur les acariens Tetranychus urticae et Phytoseiulus persimilis (Acari : Tetranychidae, Phytoseiidae présents en cultures de fraisiers du Nord du Maroc

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    Lagziri, M.

    2015-01-01

    Full Text Available Influence of previous pesticide use on Tetranychus urticae and Phytoseiulus persimilis (Acari: Tetranychidae, Phytoseiidae from strawberry crops in the north of Morocco. Description of the subject. Effects of recommended doses of five common pesticides in the strawberries of Loukkos area (Morocco were tested on the two-spotted spider mite Tetranychus urticae and its predatory mite Phytoseiulus persimilis. Laboratory study assessed the contact toxicity of one avermectin miticide (abamectin, two pyrethrinoid insecticide-acaricides (bifenthrin and lambda-cyalothrin, and two fungicides: firstly, mancozeb, which belongs to the dithiocarbamates family of chemicals and secondly, hexaconazole, of the triazole family. Objectives. The aim of the present study was to test in laboratory conditions the effect of five pesticides on the two-spotted spider mite, T. urticae and its predatory mite, P. persimilis. The five pesticides tested were those most frequently applied for at least two years on strawberries at the experimental site. Method. Bioassays were performed with populations of mites originating from different plots with various crop protection backgrounds. The first group of plots had been repeatedly treated with the five tested pesticides during a two-year period, the second group had been moderately treated, and the third had been treated once with the tested pesticides. Results. Our results showed that the tested pesticides provided effective control of T. urticae but that they were not compatible with use on the predatory mite P. persimilis, as these particular mites did not usually come into contact with these products. On the other hand, in plots where pesticides had been used for a long time, the susceptibility of P. persimilis populations to these products was significantly reduced. Conclusions. If the tested pesticides are to be considered for integrated pest control programs in plots where they have been used for a long time and where P

  1. Chemical composition and acaricidal activity of the essential oil of Baccharis dracunculifolia De Candole (1836) and its constituents nerolidol and limonene on larvae and engorged females of Rhipicephalus microplus (Acari: Ixodidae).

    Science.gov (United States)

    de Assis Lage, Tiago Coelho; Montanari, Ricardo Marques; Fernandes, Sergio Antonio; de Oliveira Monteiro, Caio Márcio; de Oliveira Souza Senra, Tatiane; Zeringota, Viviane; da Silva Matos, Renata; Daemon, Erik

    2015-01-01

    Baccharis dracunculifolia DC (common name "alecrim-do-campo" in Brazil) is a plant with widespread distribution in South America that is the botanical origin of green propolis. The aim of this study was to evaluate the chemical composition and acaricidal activity of the essential oil of B. dracunculifolia and its constituents nerolidol and limonene on unengorged larvae and engorged females of Rhipicephalus microplus (Acari: Ixodidae). The essential oil yield was 0.8% of dry mass and the major constituents were nerolidol (22.3%), germacrene D (7.2%), limonene (6.9%), β-pinene (6.7) and bicyclogermacrene (6.5%). The acaricidal activity of the essential oil and the pure compounds nerolidol and (R)-(+)-limonene were assessed in the laboratory through the modified larval packet test (LPT) and the female immersion test (FIT). In the LPT, the essential oil and nerolidol were both active, causing more than 90% mortality at concentrations from 15.0 and 10.0 mg mL(-1), respectively, whereas (R)-(+)-limonene was not active. In the FIT, the oil and nerolidol caused reduction in the quantity and quality of eggs produced, with control percentages of 96.3% and 90.3% at concentrations of 60.0 and 50.0 mg mL(-1), respectively. It can be concluded that the essential oil obtained from the aerial parts of B. dracunculifolia and its major component nerolidol have high activity on R. microplus larvae and engorged females. Copyright © 2014 Elsevier Inc. All rights reserved.

  2. Nouvelle technique d'élevage de l'acarien phyllophage Tetranychus urticae Koch (Acari : Tetranychidae et son application à l'étude de l'efficacité de quelques acaricides sur pomme de terre (Solarium tuberosum L.

    Directory of Open Access Journals (Sweden)

    Badegana, AM.

    2000-01-01

    Full Text Available A New Rearing Technique of Phytophagous Mite Tetranychus urticae Koch (Acari : Tetranychida and its Application in the Study of the Efficacy of some Acaricides on Potato [Solanum tuberosum L.. A 5 cm diameter leaf disc of potato or another host plant (or four on 2.5 cm diameter was used in a Petri dish of 9 cm diameter for the rearing technique. This leaf disc, pierced in its centre, slides along a rustproof pin and floats on a 1 mm thick lamina of demineralized fresh water. Water is a "strong barrier" which confines the tetranychid mites on the leaf disc, even if this one does not corne from a host plant (tetranychid mites deprived of food. This rearing technique was used as a bioassay to test the effectiveness of acaricides (pyrimiphos-methyl, bromopropylate, fenpropathrin, dienochlor on the developmental stages of Tetranychus urticae. The ovicidal activity against the eggs of one, three, seven days old (the eggs incubation duration being 8.1 ±0.15 days was also studied. The results obtained show that bromopropylate, fenpropathrin and dienochlor have an ovicidal activity against the eggs of the different ages, but dienochlor has the highest efficiency (90 % mortality. Pyrimiphos-methyl is only active against the seven-day old eggs and bromopropylate has a high efficiency only on the one-day old eggs. Concerning the other developmental stages such as chrysalis (protochrysalis, deu-tochrysalis, teleiochrysalis and mobile stages (larva, protonymph, deutonymph and adult female, pyrimiphos-methyl has de highest efficiency (90 % mortality ; dienochlor also, except mobile stages. Bromopropylate has no activity against the chrysalis and mobile stages and fenpropathrin has a remarkable repulsive effect.

  3. Niveles de alergenos de ácaros en el polvo de habitación en Cartagena, Colombia

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    Dilia Mercado

    1996-12-01

    Full Text Available Se investigó la cantidad de alergenos derivados de los ácaros Dermatophagoides pteronyssinus, Dermatophagoides faringe y Blomia tropicalis en el polvo de habitación de 20 casas de pacientes con asma iléigica inducida por ácaros domésticos. Las muestras se recolectaron en Cartagena, mensualmente durante un año, utilizando una aspiradora portátil modificada en el extremo succionador. Los niveles de alergenos Derp 1 y Der f 1 se determinaron mediante un inmunoensayo con anticuerpos monoclonales. La cuantificación de los alergenos totales de B. tropicalis y D. pteronyssinus se realizó mediante un ensayo de inhibición del RAST. En el polvo de colchón, el nivel más alto del alergeno Derp 1 fue 109,49 ng/g de polvo, detectado en agosto. Este nivel fue superior al doble del nivel inferior (50,3 nglg detectado en noviembre. Der f 1 se encontró solamente en tres muestras de polvo de piso. Los niveles promedio más altos de los alergenos totales de D. pteronyssinus, en el polvo de colchón, se ObSe~aron entre mayo y agosto; los niveles más bajos se presentaron entre diciembre y abril. Los niveles promedio más altos de alergenos de B. tropicalis, en el polvo de colchón, se detectaron en junio, julio y agosto, el nivel promedio más bajo se presentó en septiembre. Los niveles de alergenos en el polvo del colchón se correlacionaron con los niveles en el polvo del piso, pero, fueron significativamente más altos que éstos (p

  4. Correlation of skin test results and specific immunoglobulin E blood levels with nasal provocation testing for house-dust mite allergies.

    Science.gov (United States)

    Haxel, Boris R; Huppertz, Tilman; Boessert, Patrick; Bast, Florian; Fruth, Kai

    2016-01-01

    Allergen-specific immunotherapy for house-dust mite (HDM) allergies is associated with lower success rates when compared with similar treatments for other inhalant allergens, such as grass or birch. One reason might be the greater difficulty in diagnosing patients with assumed HDM allergies because symptoms occur perennially and may differ from those of a conventional allergic rhinitis. The aim of the study was to compare the different methods of diagnosis in patients with assumed HDM allergy. We performed a retrospective analysis of nasal provocation tests (NPT) from patients (n = 161) evaluated for Dermatophagoides pteronyssinus (n = 127) and Dermatophagoides farinae (n = 104) allergies, and compared the results with other allergen testing methods (skin-prick test [SPT], intracutaneous test, and allergen specific immunoglobulin E levels [sIgE] to detect sensitization). Receiver operating characteristic curves were used for the analyses and the areas under the curve were calculated. For D. pteronyssinus and D. farinae, 86 and 70 complete data files, respectively, were available. For both tested HDMs, the results of the receiver operating characteristic curves showed a significant correlation for SPT and sIgE, with the results of the NPT (area under the curve, 0.742 to 0.763) but not for the intracutaneous test. In patients with a positive SPT (≥3 mm), an allergy was confirmed by the NPT in 69% of cases for D. pteronyssinus and 71% for D. farinae. A positive sIgE result (ImmunoCAP class of ≥2) was verified by the NPT in 69% of cases (D. pteronyssinus) and 70% (D. farinae). The predictability value for a positive NPT result is best for SPT and sIgE. Nevertheless, even if the results of both test systems are combined, the positive predictive value that was achieved was only 0.77 for D. pteronyssinus and 0.69 for D. farinae. Therefore, in patients eligible for immunotherapy for HDM, an NPT should be performed before the start of the therapy to verify a clinically

  5. Lepidoglyphus destructor acarus in the urban house environment.

    Science.gov (United States)

    Juliá, J C; Martorell, A; Ventas, P; Cerdá, J C; Torró, I; Carreira, J; Guinot, E; Sanz, J; Alvarez, V

    1995-01-01

    To assess the presence of Lepidoglyphus destructor in the household environment of sensitized children living in an urban environment, samples of house dust were collected at the homes where two groups of patients were living, as well as in two bakeries in the city of Valencia, which were taken as a reference. Patients were divided into two groups. Group A included atopic children suffering from rhinitis and/or asthma, who were sensitized to L. destructor, as proven by prick test and specific IgE (CAP). Group B included children with the same features as those included in Group A, who were sensitized to Dermatophagoides pteronyssinus, with prick and CAP tests showing no significant sensitization to L. destructor. The samples of dust were analyzed, and the amounts of Der p I, Der f I, Der II and Lep d I per gram of dust were assessed through a solid-phase ELISA with monoclonal antibodies. In Group A, all patients but two showed a sensitization to D. pteronyssinus by prick test and serum IgE. At the homes of the patients from both groups, significant levels of Dermatophagoides were found. In Group A, only three houses showed levels of L. destructor which were comparable to those found in bakeries. Lep d I was not found in the houses of Group B patients. This means that a sensitization to L. destructor, as assessed with full extracts, is not always an indicator of its presence at the patient's house environment; it may rather refer to cross-reactivity to Dermatophagoides. Thus, availability of the main antigen Lep d I seems necessary to increase the specificity of the allergologic study.

  6. Eficiencia de dos pruebas diagnósticas en la determinación de alergia por ácaros en niños

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    Yadira Fuentes

    2008-08-01

    Full Text Available Las enfermedades alérgicas constituyen un problema de salud mundial y los ácaros se encuentran entre sus principales agentes etiológicos. En Cuba se destacan los siguientes: Dermatophagoides pteronyssinus, Dermatophagoides siboney y Blomia tropicalis. Con el objetivo de determinar la eficiencia de la prueba cutánea por punción y la determinación de IgE específica para el diagnóstico de la alergia a estos tres ácaros se desarrolló en el año 2003 un estudio analítico que incluyó 100 niños: 50 no alérgicos y 50 alérgicos que asistieron al Hospital Pediátrico "William Soler" de Ciudad de La Habana. A todos se les realizó la prueba cutánea por punción con tres extractos alergénicos (VALERGEN-DP, VALERGEN-DS y VALERGEN-BT y se les extrajo sangre para la valoración de la IgE ácaro específica; exámenes que mostraron una mayor positividad ante D. pteronyssinus en los niños enfermos. Mientras que en los no alérgicos la prueba cutánea y la IgE para D. pteronyssinus fueron negativas. La prueba cutánea presentó un 100% de especificidad, con una mayor sensibilidad para VALERGEN-DP (88%, extracto con más eficiencia diagnóstica (94%. La IgE expresó los mejores resultados de sensibilidad, especificidad y eficiencia para D. pteronyssinus (78, 100 y 89%, respectivamente. La prueba cutánea por punción fue más eficiente que la determinación de IgE específica para el diagnóstico de las alergias por ácaros. En los enfermos se observó una correlación significativa entre la respuesta cutánea y el nivel de IgE para los ácaros estudiados. Estos datos contribuyeron a mejorar el diagnóstico alergológico y alertan sobre la necesidad de extender el uso de la inmunoterapia específica con vacunas hiposensibilizantes en los niños alérgicos a los ácaros.

  7. Sensitization rates of airborne pollen and mold in children

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    So Hyun Park

    2012-09-01

    Full Text Available &lt;B&gt;Purpose:&lt;/b&gt; Aeroallergens are important causative factors of allergic diseases. Previous studies on aeroallergen sensitization rates investigated patients groups that had visited pediatric allergy clinics. In contrast, we investigated sensitization rates in a general population group of elementary school to teenage students in Incheon, Jeju, and Ulsan. &lt;b&gt;Methods:&lt;/B&gt; After obtaining parental consent, skin-prick tests were performed on 5,094 students between March and June 2010. Elementary school students were tested for 18 common aeroallergens, whereas middle and high school students were tested for 25 allergens. The 25 allergens included &lt;I&gt;Dermatophagoides pteronyssinus, Dermatophagoides farinae&lt;/I&gt;, pollen (birch, alder, oak, Japanese cedar, pine, willow, elm, maple, Bermuda grass, timothy grass, rye grass, orchard grass, meadow grass, vernal grass, mugwort, Japanese hop, fat hen, ragweed, and plantain, and mold (&lt;I&gt;Penicillatum, Aspergillus, Cladosporium, and Alternaria&lt;/I&gt;. &lt;b&gt;Results:&lt;/b&gt; The sensitization rates in descending order were 25.79% (&lt;I&gt;D. pteronyssinus&lt;/I&gt; , 18.66% (&lt;I&gt;D. farinae&lt;/I&gt; , 6.20% (mugwort, and 4.07% (willow in Incheon; 33.35% (&lt;I&gt;D. pteronyssinus&lt;/I&gt; , 24.78% (&lt;I&gt;D. farinae&lt;/I&gt;, 15.36% (Japanese cedar, and 7.33% (Alternaria in Jeju; and 32.79% (D. pteronyssinus, 30.27% (D. farinae, 10.13% (alder, and 8.68% (birch in Ulsan. The dust mite allergen showed the highest sensitization rate among the 3 regions. The sensitization rate of tree pollen was the highest in Ulsan, whereas that of Alternaria was the highest in Jeju. The ragweed sensitization rates were 0.99% in Incheon, 1.07% in Jeju, and 0.81% in Ulsan. &lt;b&gt;Conclusion:&lt;/b&gt; The

  8. Group 1 and 2 Dermatophagoides house dust mite allergens in the microenvironment of cats.

    Science.gov (United States)

    Loft, Klaus Earl; Rosser, Edmund J

    2010-04-01

    House dust mite allergens (HDMAs) are some of the most common allergens associated with allergic diseases in humans and dogs. The purpose of this study was to determine whether HDMAs could be detected in cat-associated household microenvironments. From 50 cat-only households with 95 cats, dust samples were collected by vacuuming for 2 min m(-2) from three areas where cats slept or rested regularly from September to October 2006. Relative humidity and temperature were measured in each household using a data logger. Each owner completed a questionnaire on potential factors that might influence the prevalence of house dust mites (HDMs). Dust samples were analysed utilizing an ELISA for Der p 1, Der f 1 and HDM group 2 allergens. In 38 of 50 households there was greater than 2 microg g(-1) of dust for at least one HDMA. Using stepwise logistic regression, factors associated with increased HDMA levels included: free-standing houses, number of humans in household, longhaired cats and age of the cat. Factors associated with decreased HDMA concentrations included: forced air heating and central air conditioning, less than 50% carpeting of the home, use of flea control, cats suffering from dermatological disease and the average temperature of the household. Many sleeping/resting areas utilized by cats contain sufficiently high levels of HDMAs to be potential sources of sensitization. This finding should lead to further determination of the role of HDMs in cats suffering from putative allergic conditions such as atopic dermatitis or asthma.

  9. Does IFN-γ play a role on the pathogenesis of non-atopic asthma in Latin America children?

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    Figueiredo Camila Alexandrina

    2012-12-01

    Full Text Available Abstract In this work we explore differences in blood cells and cytokine profiles in children according to atopic status and asthma (atopic or non-atopic. The study involved measurement of Th1(IFN-γ and Th2 (IL-5 and IL-13 cytokines in Dermatophagoides pteronyssinus stimulated peripheral blood leukocytes, blood cell count, skin prick test and specific IgE against common aeroallergens. Atopic status was associated with eosinophilia and production of Th2 type cytokines. Atopic asthma was associated with eosinophilia and non-atopic asthma was associated with IFN-γ and elevated monocytes in blood. IFN-γ and monocytes might play a role in immunopathology of non-atopic asthma in Latin American children.

  10. Does IFN-γ play a role on the pathogenesis of non-atopic asthma in Latin America children?

    Science.gov (United States)

    Figueiredo, Camila Alexandrina; Rodrigues, Laura Cunha; Alcantara-Neves, Neuza Maria; Cooper, Philip J; Amorim, Leila Denise; Silva, Nivea Bispo; Cruz, Alvaro A; Barreto, Mauricio Lima

    2012-12-19

    In this work we explore differences in blood cells and cytokine profiles in children according to atopic status and asthma (atopic or non-atopic). The study involved measurement of Th1(IFN-γ) and Th2 (IL-5 and IL-13) cytokines in Dermatophagoides pteronyssinus stimulated peripheral blood leukocytes, blood cell count, skin prick test and specific IgE against common aeroallergens. Atopic status was associated with eosinophilia and production of Th2 type cytokines. Atopic asthma was associated with eosinophilia and non-atopic asthma was associated with IFN-γ and elevated monocytes in blood. IFN-γ and monocytes might play a role in immunopathology of non-atopic asthma in Latin American children.

  11. Allergens involved in the cross-reactivity of Aedes aegypti with other arthropods.

    Science.gov (United States)

    Cantillo, Jose Fernando; Puerta, Leonardo; Lafosse-Marin, Sylvie; Subiza, Jose Luis; Caraballo, Luis; Fernandez-Caldas, Enrique

    2017-06-01

    Cross-reactivity between Aedes aegypti and mites, cockroaches, and shrimp has been previously suggested, but the involved molecular components have not been fully described. To evaluate the cross-reactivity between A aegypti and other arthropods. Thirty-four serum samples from patients with asthma and/or allergic rhinitis were selected, and specific IgE to A aegypti, Dermatophagoides pteronyssinus, Dermatophagoides farinae, Blomia tropicalis, Periplaneta americana. and Litopenaeus vannamei was measured by enzyme-linked immunosorbent assay. Cross-reactivity was investigated using pooled serum samples from allergic patients, allergenic extracts, and the recombinant tropomyosins (Aed a 10.0201, Der p 10, Blo t 10, Lit v 1, and Per a 7). Four IgE reactive bands were further characterized by matrix-assisted laser desorption/ionization tandem time of flight. Frequency of positive IgE reactivity was 82.35% to at least one mite species, 64.7% to A aegypti, 29.4% to P americana, and 23.5% to L vannamei. The highest IgE cross-reactivity was seen between A aegypti and D pteronyssinus (96.6%) followed by L vannamei (95.4%), B tropicalis (84.4%), and P americana (75.4%). Recombinant tropomyosins from mites, cockroach, or shrimp inhibited the IgE reactivity to the mosquito at a lower extent than the extracts from these arthropods. Several bands of A aegypti cross-reacted with arthropod extracts, and 4 of them were identified as odorant binding protein, mitochondrial cytochrome C, peptidyl-prolyl cis-trans isomerase, and protein with hypothetical magnesium ion binding function. We identified 4 novel cross-reactive allergens in A aegypti allergenic extract. These molecules could influence the manifestation of allergy to environmental allergens in the tropics. Copyright © 2017 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  12. CdTe quantum dots linked to Glutathione as a bridge for protein crosslinking

    International Nuclear Information System (INIS)

    Beato-López, J.J.; Espinazo, M.L.; Fernández-Ponce, C.; Blanco, E.; Ramírez-del-Solar, M.; Domínguez, M.; García-Cózar, F.; Litrán, R.

    2017-01-01

    We have optimized a synthetic method for the preparation of water soluble CdTe quantum dots (QDs), capped with glutathione (GSH) molecules, chemically bound to the nanoparticle surface (GSH-CdTe QDs). These QDs have been prepared by a co-precipitation reaction, in the presence of GSH. Modulating the temperature (from 90 to 145 °C) and the heating time (from 1 to 9 hours) we have obtained QDs of different sizes with a narrow size distribution, high water solubility and a fluorescent emission of a relatively high quantum yield (QY). Absorption and position of the fluorescent emission band show a strong dependence on QD size. The percentage of GSH linked to the QD surface has been estimated from chemical analysis and confirmed by thermogravimetry. The capping using this peptide, via the thiol group, converts these QDs in powerful tools as biomarkers for selective, fast and sensitive imaging in Biomedicine. The ability of these QDs to be biofunctionalized with a protein (a fundamental step for their use as biological probes) has been demonstrated. Surface functionalization of QDs is the fundamental aspect in the design of QDs for biomedical applications. In this work, the GSH-CdTe QDs have been efficiently bioconjugated with a protein extract from Dermatophagoides pteronyssinus. We have demonstrated that the GSH capping is a valuable means for subsequent protein crosslinking. Based on our results, we can conclude that proteins from Dermatophagoides pteronyssinus can be linked to GSH-CdTe QDs terminal groups. These results reveal that these GSH-capped QD probes, with high fluorescent intensity and a well functionalized surface that can be crosslinked to proteins, can have potential applications in targeted cell imaging.

  13. The influence of household pets on the composition and quantity of allergenic mite fauna within Irish homes: a preliminary investigation.

    Science.gov (United States)

    Clarke, D; Dix, E; Liddy, S; Gormally, M; Byrne, M

    2016-03-01

    Allergenic mites are responsible for inducing hypersensitive reactions in genetically predisposed people worldwide. Mites in dust from 30 Irish homes with pets (dogs, n = 23; cats, n = 7) were compared with those in 30 homes without pets. House dust mites constituted 78% of all mites recorded, with Dermatophagoides pteronyssinus (Acariformes: Pyroglyphidae) representing 57-72% of mites in furniture and mattresses in both home types compared with only 22% of mites in pet beds. Although storage mites accounted for just 13% of all mites recorded, they represented 46% of mites recorded in pet beds. Median levels of the dust mite allergen Der p 1 (µg/g) in dust samples from mattresses in homes without pets were significantly greater than in mattresses from homes with pets, reflecting the greater densities of D. pteronyssinus found in the former home category. Mite species richness was greater in homes with pets (17 species) than in homes without pets (13 species). This suggests that although the presence of pets can result in a wider variety of epidemiologically important mite species within households, increased competition among mite species may result in a more balanced mite fauna in the home, inhibiting the dominance of any one species and hence lowering allergen-associated risks. © 2015 The Royal Entomological Society.

  14. Depigmented and polymerised house dust mite allergoid: allergen content, induction of IgG4 and clinical response.

    Science.gov (United States)

    Gallego, M T; Iraola, V; Himly, M; Robinson, D S; Badiola, C; García-Robaina, J C; Briza, P; Carnés, J

    2010-01-01

    Polymerised allergenic extracts (allergoids) are commonly used in allergen immunotherapy. Clinical efficacy and safety of these extracts have been demonstrated. Recently, allergen sequences have been identified by mass spectrometry in depigmented and polymerised (Dpg-Pol) extracts. The objectives of this study were to investigate the presence of allergens in Dpg-Pol extracts of house dust mite and to analyze the immunological changes induced by these extracts in asthmatic patients enrolled in a double-blind, placebo-controlled study. Dpg-Pol extracts were manufactured and vaccines with a composition of 50% Dermatophagoides pteronyssinus and 50% D. farinae (100 HEPL/ml) were prepared. Allergen composition was analyzed by mass spectrometry. Patients with asthma and rhinoconjunctivitis were treated in a 1-year, double-blind, placebo-controlled, parallel-group study with 6 up-dosing and monthly maintenance injections. Specific IgE and IgG4 titres to D. pteronyssinus, Der p 1 and Der p 2 were measured in patients' sera using the CAP system and direct ELISA experiments. Sequences from the major allergens Der p 1 and Der p 2 and from other allergens were identified in native and Dpg-Pol extracts. There was a statistically significant increase in specific IgG4, a decrease in the ratio of IgE/IgG4 to D. pteronyssinus and a significant increase in specific IgG4 to Der p 1 and Der p 2 in the patients allotted to active treatment. The detection of allergen sequences suggests preservation of major and minor allergens in Dpg-Pol allergoids from house dust mites. Efficacy in asthma treatment and the increase in specific IgG4 seem to be associated with the presence of major allergens in Dpg-Pol allergen extracts. Copyright (c) 2010 S. Karger AG, Basel.

  15. Sensitization to 10 mites in a tropic area. Der p and Der f are important risk factor for sensitization to other mites from Pyroglyphidae, Acaridae, Chortoglyphidae, and Glyciphagidae families

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    Jorge Sánchez

    2017-06-01

    Full Text Available Background: Much is known about the frequency of sensitization to Blomia tropicalis, Dermatophagoides pteronyssinus and Dermatophagoides farinae, although less is known about sensitization to other species and their possible interactions. Objective: In patients with allergic manifestations, to evaluate the frequency of sensitization to 10 species of mites in a tropical area and their possible interactions. Methods: Cross-sectional study. Sensitization was evaluated by skin tests. A generalized linear Poisson regression model with robust variance was used. Based on the sensitization probability reasons and social networking analysis, explorations of relationship for 10 mites were performed. Results: 147 patients were included. The highest sensitization was found to mites' family Pyroglyphidae (> 70 % and less frequently was the Glycyphagidae family (< 50 %. Sensitization to any mites significantly increased the likelihood of sensitization to others. Sensitization to Der f or Der p increased, more than 20 times the likelihood of sensitization to other mites of the Pyroglyphidae family and more than 10 times to mites from other families. Sensitization to mites from Glycyphagidae, Chortoglyphidae or Acaridae family also increased the risk of sensitization to other mites but less than 5 times. Conclusion: Sensitization to mites is frequent in tropical area. Pyroglyphidae sensitization is the main risk factor for polysensitization with other mites from Glycyphagidae, Chortoglyphidae or Acaridae. These results must be considered at diagnosis and treatment of allergy diseases.

  16. [Prevalence of sensitization to inhalant allergens in the immunology department from Hospital de Especialidades Pediátricas María, Honduras, 2016].

    Science.gov (United States)

    Gonzales-González, Victoria Alejandra; Díaz-Flores, Adolfo Martín; Fernández-Zelaya, Karla Zobeyda; Rivera-Reyes, María Félix

    2017-01-01

    Allergic diseases are a public health problem; estimates indicate that between 30% and 40% of the world population is affected by some allergy. Knowing the prevalence of allergen sensitization allows for adequate diagnoses and treatments to be offered. In Honduras there are no studies available in pediatric patients. The purpose of this research was to identify the most common types of sensitization in children and their correlation with the most common allergic diseases in patients on immunotherapy at the Maria Hospital of Pediatric Specialties. Cross-sectional, descriptive, retrospective study in which medical records and databases of patients on allergic immunotherapy at the Maria Hospital of Pediatric Specialties were reviewed between January 2015 and June 2016. 215 children on immunotherapy were assessed; ages ranged from 3 to 18 years, with a mean of 10.8 years. Aeroallergen-positive epicutaneous tests were identified in 73.02%. The most common aeroallergens were Dermatophagoides farinae and Dermatophagoides pteronyssinus mix (96.74%), American cockroach 37.21%, and Aspergillus fumigatus and Homodenderum cladosporioides (16.28%). House dust mites and American cockroach were the most common causes of sensitization in patients on immunotherapy.

  17. Oral mite anaphylaxis caused by mite-contaminated okonomiyaki/ pancake-mix in Japan: 8 case reports and a review of 28 reported cases.

    Science.gov (United States)

    Takahashi, Kentaro; Taniguchi, Masami; Fukutomi, Yuma; Sekiya, Kiyoshi; Watai, Kentaro; Mitsui, Chihiro; Tanimoto, Hidenori; Oshikata, Chiyako; Tsuburai, Takahiro; Tsurikisawa, Naomi; Minoguchi, Kenji; Nakajima, Hiroshi; Akiyama, Kazuo

    2014-03-01

    Anaphylaxis after the ingestion of foods contaminated with mites has recently been recognized. Case series and case reports thus far have shown that mite-contaminated wheat flour is the major cause of oral mite anaphylaxis. However, we have found 8 cases of oral mite anaphylaxis which were caused by mite-contaminated okonomiyaki-mix, a savory Japanese style pancake mix, in our hospital. In addition to our 8 cases, the databases of MEDLINE and ICHUSHI were systematically searched for patients with oral mite anaphylaxis in Japan. Thirty-six patients including our 8 cases with oral mite anaphylaxis were identified. Thirty-four out of 36 cases (94%) ingested okonomiyaki or takoyaki, prepared at home using okonomiyaki-mix or takoyaki-mix which was previously opened and stored for months at ambient temperature. Microscopic examination of culprit mixes of 16 cases including our 1 case revealed contamination of mites such as Dermatophagoides farina (Der f) (5 cases), Tyrophagus putrescentiae (Tyr p) (4 cases), and Dermatophagoides pteronyssinus (Der p) (3 cases). The specific IgE to each mite is generally upregulated in these patients. Especially, the titers of specific IgE to Der p and Der f were more than class 2 in all cases. Mite-contaminated flavored flour is the major cause of oral mite anaphylaxis in Japan.

  18. Sublingual immunotherapy (SLIT – indications, mechanism, and efficacy Position paper prepared by the Section of Immunotherapy, Polish Society of Allergy

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    Marek Jutel

    2015-12-01

    Full Text Available SLIT ( sublingual immunotherapy induces allergen-specific immune tolerance by sublingual administration of a gradually increasing dose of an allergen. The mechanism of SLIT is comparable to those during SCIT (subcutaneous immunotherapy, with the exception of local oral dendritic cells, pre-programmed to elicit tolerance. In the SLIT dose, to achieve the same efficacy as in SCIT, it should be 50–100 times higher with better safety profile. The highest quality evidence supporting the efficacy of SLIT lasting 1 – 3 years has been provided by the large scale double-blind, placebo-controlled (DBPC trials for grass pollen extracts, both in children and adults with allergic rhinitis. Current indications for SLIT are allergic rhinitis (and conjunctivitis in both children and adults sensitized to pollen allergens (trees, grass, Parietaria , house dust mites ( Dermatophagoides pteronyssinus, Dermatophagoides farinae , cat fur, as well as mild to moderate controlled atopic asthma in children sensitized to house dust mites. There are positive findings for both asthma and new sensitization prevention. Severe adverse events, including anaphylaxis, are very rare, and no fatalities have been reported. Local adverse reactions develop in up to 70 – 80% of patients. Risk factors for SLIT adverse events have not been clearly identified. Risk factors of non-adherence to treatment might be dependent on the patient, disease treatment, physician-patient relationship, and variables in the health care system organization.

  19. Association between HLA genes and dust mite sensitivity in a Brazilian population.

    Science.gov (United States)

    da Costa Lima Caniatti, Marcela Caleffi; Borelli, Sueli Donizete; Guilherme, Ana Lúcia Falavigna; Tsuneto, Luiza Tamie

    2017-02-01

    Type I hypersensitivity, also known as IgE-mediated allergy, is a complex, multifactorial condition whose onset and severity are influenced by both genetic and environmental factors. Mite allergens stimulate the production of humoral response (IgE), especially in children, which is closely involved in atopic asthma and rhinitis. This study aimed to investigate the association between HLA class I (-A, -B, and -C), and HLA class II (-DRB1) genes in individuals sensitive to dust mites (Dermatophagoides farinae, Dermatophagoides pteronyssinus, or Blomia tropicalis) and mite-insensitive controls. 396 participants were grouped as mite-sensitive and mite-insensitive according to immediate hypersensitivity as determined by skin-prick tests, and to HLA genotyping by polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO). After chi-square heterogeneity testing no significant differences were observed in HLA-A, B, and C genes, except for the HLA-DRB1 locus, which, showed a negative association for DRB1∗04, between mite-sensitive and mite-insensitive individuals. In high resolution, DRB1∗04:11 allele was significantly different from all other results (P=0.0042, OR=0.26, and 95%CI=0.09-0.70). The analysis stratified by etiologic agent confirmed these associations. Our results suggest a possible association between HLA-DRB1 genes and hypersensitivity to dust mites. Copyright © 2016 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

  20. Association between KIR genes and dust mite sensitization in a Brazilian population.

    Science.gov (United States)

    Caniatti, Marcela Caleffi da Costa Lima; Borelli, Sueli Donizete; Guilherme, Ana Lúcia Falavigna; Franzener, Soraya Barrionuevo; Tsuneto, Luiza Tamie

    2018-01-01

    Killer cell immunoglobulin-like receptors (KIRs), found on the surface of natural killer (NK) cells, play a key role in controlling the innate response. Such response depends on a series of cellular interactions between these receptors and HLA activating/inhibiting ligands. Atopic diseases have been associated with genes that regulate cytokine production and HLA genes, which may either protect or predispose to hypersensitivity. To verify an association study of KIR genes with sensitization to the following mites: Dermatophagoides farinae, Dermatophagoides pteronyssinus, and Blomia tropicalis. A total of 341 children aged up to 14 years, were classified as mite-sensitive or mite-insensitive after undergoing a skin prick test for immediate allergic reactions. The presence/absence of KIR genes and their human leukocyte antigen (HLA) ligands was determined by polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO) with the commercial kit LabType™ using Luminex™. The frequencies of KIR genes and their respective class I HLA ligands and the frequency of haplotypes were performed in sensitive and insensitive individuals, and no significant differences were found. Our results suggest no influence of KIR genes on resistance/susceptibility to sensitization to dust mites. Copyright © 2017 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

  1. Levels of house dust mite-specific serum immunoglobulin E (IgE) in different cat populations using a monoclonal based anti-IgE enzyme-linked immunosorbent assay.

    Science.gov (United States)

    Bexley, Jennifer; Hogg, Janice E; Hammerberg, Bruce; Halliwell, Richard E W

    2009-10-01

    Levels of serum immunoglobulin E (IgE) specific for the house dust mites (HDMs) Dermatophagoides farinae (DF) and Dermatophagoides pteronyssinus (DP) in 58 cats with clinical signs suggestive of atopic dermatitis (allergic dermatitis cats), 52 cats with no history of allergic or immunological disease (nonallergic cats) and 26 specific pathogen-free (SPF) cats were measured using a monoclonal anti-IgE enzyme-linked immunosorbent assay. Reactivity to both native and reduced HDM allergens was compared. SPF cats had significantly lower levels of HDM-specific serum IgE than cats with allergic dermatitis and nonallergic cats. The difference in levels of HDM-specific IgE in the serum of cats with allergic dermatitis and nonallergic cats was significant for native DF allergen, but not for native DP allergen or reduced HDM allergens. The results suggest that DF in its native form may be a significant allergen in cats with allergic dermatitis. The clinical relevance of these reactions, however, remains to be proven.

  2. Our experience with atopy patch tests with aeroallergens.

    Science.gov (United States)

    Celakovská, Jarmila; Ettlerová, Kvetuse; Ettler, Karel; Vanecková, Jaroslava

    2013-01-01

    Aim of our study was to evaluate the importance of atopy patch testing with aeroallergens as a diagnostic method in patients suffering from atopic dermatitis. The complet dermatological and allergological examinations were performed in 29 patients; 10 men, 19 women with the average age of 27.8 years, min. 17, max. 57 years; with the median SCORAD 24.2 points, s.d. 13.3 points. Wormwood, grass, dog dander, cat dander, dermatophagoides pharinae, dermatophagoides pteronyssinus and birch pollen were examined in diagnostic procedures. Skin prick tests, specific IgE were examined; the atopy patch tests were performed with aeroallergens for skin prick tests in concentration 1 x skin prick tests. Specific IgE and skin prick tests to one or more tested aeroallergens were positive altogether in 27 patients; atopy patch tests were positive only in one of these patients. For atopy patch testing with aeroallergens the concentration of 1 x skin prick tests is low to confirme the eczematic reaction in patients suffering from allergy to inhallant allergens.

  3. Prevalence of sensitization to inhalant allergens in a immunology department from Tegucigalpa, Honduras, 2016

    Directory of Open Access Journals (Sweden)

    Victoria Alejandra Gonzales-González

    2017-02-01

    Full Text Available Background: Allergic diseases are a public health problem; estimates indicate that between 30% and 40% of the world population is affected by some allergy. Knowing the prevalence of allergen sensitization allows for adequate diagnoses and treatments to be offered. In Honduras there are no studies available in pediatric patients. Objectives: The purpose of this research was to identify the most common types of sensitization in children and their correlation with the most common allergic diseases in patients on immunotherapy at the Maria Hospital of Pediatric Specialties. Methods: Cross-sectional, descriptive, retrospective study in which medical records and databases of patients on allergic immunotherapy at the Maria Hospital of Pediatric Specialties were reviewed between January 2015 and June 2016. Results: 215 children on immunotherapy were assessed; ages ranged from 3 to 18 years, with a mean of 10.8 years. Aeroallergen-positive epicutaneous tests were identified in 73.02%. The most common aeroallergens were Dermatophagoides farinae and Dermatophagoides pteronyssinus mix (96.74%, American cockroach 37.21%, and Aspergillus fumigatus and Homodenderum cladosporioides (16.28%. Conclusions: House dust mites and American cockroach were the most common causes of sensitization in patients on immunotherapy.

  4. Comparison and clinical utility evaluation of four multiple allergen simultaneous tests including two newly introduced fully automated analyzers

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    John Hoon Rim

    2016-04-01

    Full Text Available Background: We compared the diagnostic performances of two newly introduced fully automated multiple allergen simultaneous tests (MAST analyzers with two conventional MAST assays. Methods: The serum samples from a total of 53 and 104 patients were tested for food panels and inhalant panels, respectively, in four analyzers including AdvanSure AlloScreen (LG Life Science, Korea, AdvanSure Allostation Smart II (LG Life Science, PROTIA Allergy-Q (ProteomeTech, Korea, and RIDA Allergy Screen (R-Biopharm, Germany. We compared not only the total agreement percentages but also positive propensities among four analyzers. Results: Evaluation of AdvanSure Allostation Smart II as upgraded version of AdvanSure AlloScreen revealed good concordance with total agreement percentages of 93.0% and 92.2% in food and inhalant panel, respectively. Comparisons of AdvanSure Allostation Smart II or PROTIA Allergy-Q with RIDA Allergy Screen also showed good concordance performance with positive propensities of two new analyzers for common allergens (Dermatophagoides farina and Dermatophagoides pteronyssinus. The changes of cut-off level resulted in various total agreement percentage fluctuations among allergens by different analyzers, although current cut-off level of class 2 appeared to be generally suitable. Conclusions: AdvanSure Allostation Smart II and PROTIA Allergy-Q presented favorable agreement performances with RIDA Allergy Screen, although positive propensities were noticed in common allergens. Keywords: Multiple allergen simultaneous test, Automated analyzer

  5. Efficacy evaluation of a pollen blocker cream against dust-mite allergy: A multicenter, randomized, double-blind, placebo-controlled crossover trial.

    Science.gov (United States)

    Li, Yanqing; Cheng, Lei; Chen, Xiaoning; Yang, Beibei; Wang, Dehui

    2015-01-01

    To further evaluate the efficacy and safety of a pollen blocker cream against dust-mite allergy. A multicenter, randomized, double-blind, placebo-controlled, crossover trial was conducted in a Chinese population. Patients diagnosed with perennial allergic rhinitis, sensitive to dust-mite allergy including Dermatophagoides farinae and Dermatophagoides pteronyssinus were randomly allocated to receive a pollen blocker cream or placebo, which was applied and spread evenly to the lower internal nose region three times daily for a total of 30 days. The primary outcome measurements for efficacy were total nasal symptom score (TNSS) and individual nasal symptom score (iNSS). Adverse events were also monitored. After application of a pollen blocker, the mean TNSS decreased from 23.1 to 13.8, the decrease of the pollen blocker group (9.3) was highly significant compared with the placebo group (5.2; p 0.05), and no severe systematic reactions were observed. Pollen Blocker is a safe and effective alternative to the drugs for treatment of AR, especially for Chinese people allergic to dust-mite allergy.

  6. The skin prick test – European standards

    Directory of Open Access Journals (Sweden)

    Heinzerling Lucie

    2013-02-01

    Full Text Available Abstract Skin prick testing is an essential test procedure to confirm sensitization in IgE-mediated allergic disease in subjects with rhinoconjunctivitis, asthma, urticaria, anapylaxis, atopic eczema and food and drug allergy. This manuscript reviews the available evidence including Medline and Embase searches, abstracts of international allergy meetings and position papers from the world allergy literature. The recommended method of prick testing includes the appropriate use of specific allergen extracts, positive and negative controls, interpretation of the tests after 15 – 20 minutes of application, with a positive result defined as a wheal ≥3 mm diameter. A standard prick test panel for Europe for inhalants is proposed and includes hazel (Corylus avellana, alder (Alnus incana, birch (Betula alba, plane (Platanus vulgaris, cypress (Cupressus sempervirens, grass mix (Poa pratensis, Dactilis glomerata, Lolium perenne, Phleum pratense, Festuca pratensis, Helictotrichon pretense, Olive (Olea europaea, mugwort (Artemisia vulgaris, ragweed (Ambrosia artemisiifolia, Alternaria alternata (tenuis, Cladosporium herbarum, Aspergillus fumigatus, Parietaria, cat, dog, Dermatophagoides pteronyssinus, Dermatophagoides farinae, and cockroach (Blatella germanica. Standardization of the skin test procedures and standard panels for different geographic locations are encouraged worldwide to permit better comparisons for diagnostic, clinical and research purposes.

  7. House dust mites on skin, clothes, and bedding of atopic dermatitis patients.

    Science.gov (United States)

    Teplitsky, Valery; Mumcuoglu, Kosta Y; Babai, Ilan; Dalal, Ilan; Cohen, Rifka; Tanay, Amir

    2008-08-01

    Atopic dermatitis is a common allergic condition in children, often associated with a positive skin reaction to house dust mite allergens. To determine the presence of house dust mites on the skin, clothes, and bedding of patients with atopic dermatitis. Nineteen patients with atopic dermatitis were examined during a 2-year period. Samples from affected and healthy skin surfaces were obtained with adhesive tape, and dust samples from bedding and clothes were collected with a vacuum cleaner at the start of the study and 3-6 weeks later, and examined for the presence of house dust mites. The findings were compared with those of 21 healthy controls. The most common mite species on skin were Dermatophagoides pteronyssinus and Dermatophagoides farinae, which were found in nine patients and three controls. The patient group showed a significantly larger percentage of samples with mites than did the control group (34.9% and 7.9%, respectively) (P bedding of patients, or between patients and controls with regard to the number of mites on the clothes and bedding. Patients with atopic dermatitis showed a higher prevalence of mites on their skin than did healthy individuals, which could be involved in allergic sensitization and disease exacerbation.

  8. Sensitization Profile to Allergens in Patients Using Multi-Test II

    Science.gov (United States)

    Maniglia, Sergio Fabricio; Tsuru, Fernanda Miyoko; Santos, Victor Carvalho dos; Ueda, Denis Massatsugu

    2014-01-01

    Introduction Medical intervention in allergies has broadened its perspective, also focusing in the quality of life of patients. Patients are instructed, before using pharmacotherapy agents, to avoid the causal agent. Objective This study aims to analyze the sensitization profile of patients with allergic complaints and identify possible characteristics specific to each age group and gender. Methods A descriptive cross-sectional study included data collected from medical records (from Multi-Test II database, Lincoln Diagnostics Inc. Decatur, Illinois) of 1,912 patients who underwent skin prick test from March to October 2013. Patients were organized and analyzed according to gender, age, and results of the allergens subtypes tested. Results The study was composed of 1,912 patients (60% male and 40% female) of ages between 3 and 87 years. Positive tests were more prevalent in quantity and intensity with the mites Dermatophagoides pteronyssinus and Dermatophagoides farinae, each with 60% of the total analyzed. In second place were pollens, especially Dactylis glomerata and Festuca pratensis. Conclusion The female and male sexes were equally atopic. Fungi and epithelia of dog and cat were not considered potential aeroallergens that could cause symptoms. However, mites are common in Paraná, Brazil. Further studies regarding the pollens are needed, as this study result diverged from the literature. PMID:25992129

  9. New Neoribates (Acari, Oribatida, Parakalummidae) from Vietnam

    Czech Academy of Sciences Publication Activity Database

    Ermilov, S.G.; Starý, Josef

    2017-01-01

    Roč. 4303, č. 1 (2017), s. 51-72 ISSN 1175-5326 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Oripodoidea * morphology * systematics * Tam Dao National Park * Oriental region Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 0.972, year: 2016

  10. New Protoribates (Acari, Oribatida, Haplozetidae) from Vietnam

    Czech Academy of Sciences Publication Activity Database

    Ermilov, S.G.; Starý, Josef

    2017-01-01

    Roč. 4258, č. 6 (2017), s. 501-524 ISSN 1175-5326 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Oripodoidea * morphology * systematics * new species * Tam Dao National Park * Oriental region Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 0.972, year: 2016

  11. fabricius (acari: ixodidae en equinos y bovinos

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    Víctor Álvarez C.

    2007-01-01

    Full Text Available Con el objetivo de ampliar el conocimiento sobre el comportamiento de los adultos y las ninfas de Amblyomma en equinos y bovinos, se desarrolló un estudio en San Pablo de Turrubares, provincia de San José. Para el trabajo de campo se empleó 3 métodos: revisión de los animales seleccionados, el arrastre por medio de bandera, y el trampeo con CO2. Los equinos demostraron ser la especie más susceptible para A. cajennense, seguidos por B. taurus. Las ninfas de A. cajennense se concentran en la época seca y a nivel de laboratorio muestran indicadores que colocan a estas garrapatas como individuos con una gran capacidad para sobrevivir sin alimento, lo que dificulta su control. No se encontró correlación entre la precipitación y los niveles de garrapatas; sin embargo, durante la época que incluye la mayoría de los meses lluviosos se encontró una mayor cantidad de garrapatas. Se observó sitios de preferencia por parte de A. cajennense para fijarse al cuerpo de los animales.

  12. A revision of the Plateremaeidae (Acari: Oribatei

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    Adilson D. Paschoal

    1986-01-01

    Full Text Available The family Plateremaeidae is revised, and includes the following taxa: Plateremaeus Berlese, with the only species P. ornatissimus (Berlese, from Brazil; Allodamaeus Banks, with the species ewingi (Banks, from the USA, coralgablensis, sp. n. (type-locality: USA, Flórida, Coral Gables, and ornatos Balogh & Csiszár, from Argentina; Lophoremaeus, gen. n., with two species: mirabilis Csiszár, from Bulgaria, the type-species, and laminipes (Berlese, n. comb., from Italy; Paralopheremaeus, gen. n., with the species legendrei (Balogh, n. comb., from Madagascar; Calipteremaeus, gen. n., with the species yaginumai (Aoki, n. comb., from Japan; the following species are considered incertae sedis: Plateremaeus carinulatus (Berlese, from Brasil, P. complanatus (Berlese, from Chile, P. rotundatus Berlese, from Japan, and P. tunicatus ( Balogh, from Zaire.A família Plateremaeidae é revista, e inclui os seguintes táxons: Plateremaeus Berlese, com a única espécie P. ornatissimus (Berlese, do Brazil; Allodamaeus Banks, com as espécies ewingi (Banks, dos Estados Unidos, coralgablensis, sp. n. (localidade-tipo: Estados Unidos, Flórida, Coral Gables e ornatos Balogh & Csziszár, da Argentina; Lophoremaeus, gen. n., com duas espécies: mirabilis Csiszár, da Bulgária, espécie-tipo, e laminipes (Berlese, n. comb., da Itália; Paralopheremaeus, gen. n., com a espécie legendrei (Balogh, n. comb., de Madagascar; Calipteremaeus. gen. n., com a espécie yaginumai (Aoki, n. comb., do Japão; as seguintes espécies são consideradas incertae sedis: Plateremaeus carinulatus (Berlese, do Brasil, P. complanatus (Berlese, do Chile, P. rotundatus Berlese, do Japão, e P. tunicatus (Balogh, do Zaire.

  13. House Dust Mite Prevalence in the House of Patients with Atopic Dermatitis in Mashhad, Iran

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    Toktam Ziyaei

    2017-06-01

    Full Text Available Background: Being exposed to house dust mites intensifies atopic dermatitis. This study has investigated the con­tamination rate with Dermatophagoides mites in patient's residential home with atopic dermatitis.Methods: In this cross-sectional study, 40 patients took part with atopic dermatitis (positive or negative for mites by prick Dermal Test. Samples were collected from 3 locations (living room, bedroom and bed by vacuum cleaner. Dust samples (transferred to freezer -20 ˚C were examined by direct method and flotation. The data were analyzed using statistical SPSS vr.20 software.Results: Twenty patients of positive prick test included 8 (40% male and 12 (60% female. The results of direct observation of mites: 7 cases (35% in bedding sheets, 6 cases (30% bedrooms' carpet, 3 cases (15% living room's carpet. Twenty patients of negative prick test included 8 (40% male and 12 (60% female. Only mites were found (5% in living room's carpets of negative prick test patients. Dermatophagoides pteronyssinus was more frequent than Dermatophagoides farinae. (98% vs 83%.Conclusion: Fifty-five percent of residential homes of prick test positive patients and only 5% of residential homes of prick test negative patients were positive for mite. Sunshine provided home had fewer mites than home where sunshine is not provided. Prick test positive patients used handmade carpets more than machine made ones. In posi­tive prick test patients, mites were found in bed sheet and bedroom’s carpet more than negative prick test patient's sheets and carpets.

  14. Evaluation of skin sensitivity in dogs bearing allergic dermatitis to standardized allergenic extract of house dust and storage mites Avaliação da sensibilidade de cães com dermatite alérgica a extratos padronizados de ácaros da poeira domiciliar

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    Victor E.S. Cunha

    2007-08-01

    Full Text Available The objective of the study was to evaluate whether allergenic extracts of five house dust and storage mite species standardized for humans might be used for the diagnosis of canine atopic dermatitis (CAD. Extracts of Dermatophagoides pteronyssinus (Pyroglyphidae, D. farinae (Pyroglyphidae, Blomia tropicalis (Glycyphagidae, Lepidoglyphus destructor (Glycyphagidae and Tyrophagus putrescentiae (Acaridae were evaluated by intradermal testing in 20 healthy dogs (control and 25 dogs with allergic dermatitis. A significant difference in the response was observed between the two groups (pO presente trabalho teve como objetivo avaliar se extratos alergênicos de cinco espécies de ácaros da poeira domiciliar e produtos armazenados, padronizados para humanos, podem ser utilizados no diagnóstico da dermatite atópica canina. Extratos de Dermatophagoides pteronyssinus (Pyroglyphidae, D. farinae (Pyroglyphidae, Blomia tropicalis (Glycyphagidae, Lepidoglyphus destructor (Glycyphagidae e Tyrophagus putrescentiae (Acaridae foram avaliados através de testes intradérmicos em 45 cães, dos quais 20 normais e 25 com dermatite alérgica. Uma diferença significativa foi observada no padrão de respostas obtidas dos dois grupos (p<0.05. Apenas um animal (5% do grupo controle reagiu ao teste cutâneo, enquanto que no grupo dos alérgicos 14 cães (56% apresentaram pelo menos uma reação positiva (odds ratio = 24.2. As maiores freqüências de reações positivas observadas no grupo dos alérgicos foram aos extratos de T. putres-centiae ou L. destructor, cada um induzindo reações em 10(40% cães. Os extratos de D. farinae, D. pteronyssinus e B. tropicalis foram responsáveis por reações positivas em 7(28%, 3(12% e 3(12% cães, respectivamente. Os extratos padronizados para humanos avaliados no presente estudo podem ser utilizados como complemento no diagnóstico da doença, assim como na seleção de alérgenos para a imunoterapia alérgeno-específica.

  15. Asthma, Airway Symptoms and Rhinitis in Office Workers in Malaysia: Associations with House Dust Mite (HDM Allergy, Cat Allergy and Levels of House Dust Mite Allergens in Office Dust.

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    Fang Lee Lim

    Full Text Available A prevalence study was conducted among office workers in Malaysia (N= 695. The aim of this study was to examine associations between asthma, airway symptoms, rhinitis and house dust mites (HDM and cat allergy and HDM levels in office dust. Medical data was collected by a questionnaire. Skin prick tests were performed for HDM allergens (Dermatophagoides pteronyssinus, Dermatophagoides farinae and cat allergen Felis domesticus. Indoor temperature and relative air humidity (RH were measured in the offices and vacuumed dust samples were analyzed for HDM allergens. The prevalence of D. pteronyssinus, D. farinae and cat allergy were 50.3%, 49.0% and 25.5% respectively. Totally 9.6% had doctor-diagnosed asthma, 15.5% had current wheeze and 53.0% had current rhinitis. The Der p 1 (from D. pteronyssinus and Der f 1 (from D. farinae allergens levels in dust were 556 ng/g and 658 ng/g respectively. Statistical analysis was conducted by multilevel logistic regression, adjusting for age, gender, current smoking, HDM or cat allergy, home dampness and recent indoor painting at home. Office workers with HDM allergy had more wheeze (p= 0.035, any airway symptoms (p= 0.032, doctor-diagnosed asthma (p= 0.005, current asthma (p= 0.007, current rhinitis (p= 0.021 and rhinoconjuctivitis (p< 0.001. Cat allergy was associated with wheeze (p= 0.021, wheeze when not having a cold (p= 0.033, any airway symptoms (p= 0.034, doctor-diagnosed asthma (p= 0.010, current asthma (p= 0.020 and nasal allergy medication (p= 0.042. Der f 1 level in dust was associated with daytime breathlessness (p= 0.033 especially among those with HDM allergy. Der f 1 levels were correlated with indoor temperature (p< 0.001 and inversely correlated with RH (p< 0.001. In conclusion, HDM and cat allergies were common and independently associated with asthma, airway symptoms and rhinitis. Der f 1 allergen can be a risk factor for daytime breathlessness.

  16. Identificación de ácaros del polvo casero en colchones y almohadas de niños alérgicos de Santa Marta, Colombia

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    Jainy Meza Navarro

    2013-10-01

    Full Text Available ResumenLa prevalencia elevada de alergias en Colombia hace necesario investigar los factores que influye sobre su etiología. El objetivo de este estudio fue identificar la fauna de ácaros presente en los hogares de niños alérgicos de Santa Marta. Un total de 70 muestras de polvo fueron colectadas desde 42 casas por medio de la aspiración de colchones y almohadas, durante los meses de Julio a Octubre de 2007. Los ácaros fueron identificados bajo la luz de un microscopio usando claves taxonómicas, contados y expresados como ácaros por gramo de polvo. Los ácaros de la familia Pyroglyphidae fueron los más predominantes (84,58%, teniendo a Dermatophagoides farinae (23,85% como la especie más abundante, seguido por Dermatophagoides pteronyssinus (7,31%. Otros ácaros prevalentes fueron Cheyletus spp, Euroglyphus maynei y Blomia tropicales, con niveles superiores a los 500 ácaros/ gramo, considerado como de alto riesgo para sensibilización alérgica. Estos resultados contribuyen al conocimiento de la fauna de ácaros del polvo casero de Santa Marta, importante para el diagnóstico y terapia de las alergias. (Duazary 2008; 1: 24 - 31.AbstractThe increasing prevalence of allergic diseases in Colombia makes necessary to research about factors that influence their etiology. The aim of this study was to investigate the mite fauna present in homes of allergic child from Santa Marta. A total of 70 samples were collected from 42 houses through vacuuming mattresses and pillows, during the months of July to October 2007. Mites were identified under light microscopy using taxonomic keys, count and expressed as mites per gram of dust. The prevalent mite family was Pyroglyphidae (84.58%, having Dermatophagoides farinae as the major specie (23.85%, followed by D. pteronyssinus. Other mites prevalent were Cheyletus spp, Euroglyphus maynei and Blomia tropicalis, with levels up to 500 mites/ gram, considered like of high risk for the allergic sensitization

  17. Testes cutâneos de hipersensibilidade imediata com o evoluir da idade Positive skin test and age

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    Wilma Carvalho Neves Forte

    2001-04-01

    Full Text Available OBJETIVO: avaliação da positividade aos testes cutâneos de hipersensibilidade imediata em crianças com asma brônquica e/ou rinite alérgica em diferentes faixas etárias. CASUÍSTICA E MÉTODOS: foi observada a positividade aos testes cutâneos de hipersensibilidade imediata, por testes de puntura, frente a diferentes alérgenos de mesma procedência: poeira total e Dermatophagóides sp, Dermatophagoides pteronyssinus, Dermatophagoides farinae e Blomia tropicalis, Penicillium sp, Alternaria alternata, Cladosporium herbarium, Aspergillus fumigatus, grama bermuda, capim de pasto, epitélio de cão, epitélio de gato, penas, Blatella germanica, lã. Foram selecionadas 713 crianças divididas em grupos conforme a faixa etária: grupo I (6 a 11 meses, II (1 a 3 anos e 11 meses, III (4 a 8 anos e 11 meses e IV (9 a 15 anos. Para análise estatística utilizou-se o cálculo do qui-quadrado. RESULTADOS: o total de diferenças significativas entre os vários grupos foi: I e II = 5; II e III = 5; II e IV = 5; III e IV = 6; I e III = 10 e I e IV = 10 CONCLUSÃO: concluiu-se que a positividade ao teste de hipersensibilidade imediata foi maior com o evoluir da idade, havendo positividade já aos doze meses de vida, sendo esta positividade significativamente maior a partir de quatro anos de idade.OBJECTIVE: to evaluate positive responses to skin tests for immediate hypersensitivity to allergens in children with asthma and rhinitis at different ages. METHOD: we observed positive skin test reactivity in prick tests using fifteen allergens of same origin (total dust and Dermatophagoides sp.; Dermatophagoides pteronyssinus; Dermatophagoides farinae; Blomia tropicalis; Penicillium sp; Alternaria alternata; Cladosporium herbarium; Aspergillus fumigatus; Bermuda grass; forage grass; dog and cat epithelia; feathers; Blatella germanica and wool. We placed 713 selected patients into different age groups - Group I: 6 to 11 months; Group II: 1 to 3 years and 11

  18. Caracterização de um vírus baciliforme isolado de Solanum violaefolium transmitido pelos ácaros Brevipalpus phoenicis e Brevipalpus obovatus (Acari: Tenuipalpidae Characterization of a bacilliform virus isolated from Solanum violaefolium transmitted by the tenuipalpid mites Brevipalpus phoenicis and Brevipalpus obovatus

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    Paulo de Tarso Oliveira Ferreira

    2007-09-01

    Full Text Available Solano-violeta (Solanum violaefolium é uma planta ornamental rasteira usada para cobrir solos de áreas sombreadas. Um vírus que induz manchas anelares nas folhas desta planta, tentativamente designado Solanum violaefolium ringspot virus - SvRSV, transmitido pelo ácaro Brevipalpus phoenicis (Acari: Tenuipalpidae foi encontrado em Piracicaba, SP. Trata-se de um vírus baciliforme que se assemelha a outros vírus do tipo citoplasmático transmitidos por Brevipalpus sp. Este trabalho teve como objetivo relatar propriedades biológicas e estabelecer uma caracterização molecular parcial do SvRSV. O vírus pode ser transmitido mecanicamente a várias outras espécies botânicas, causando lesões localizadas. Entre as espécies avaliadas, Datura stramonium mostrou-se a melhor hospedeira experimental. Observou-se também a manifestação de sintomas nestas plantas após infestação das mesmas por B. obovatus previamente alimentado em lesões de SvRSV, confirmando esta outra espécie de ácaro como vetor do vírus. Suas propriedades físicas in vitro foram: temperatura de inativação 40-45 ºC; ponto final de diluição 10-3-10-4; longevidade in vitro 12 dias. Em secções ultrafinas, as partículas do SvRSV mostraram-se levemente mais delgadas e mais longas que as de outros vírus do mesmo grupo. A partir do dsRNA do SvRSV foi construída uma biblioteca de cDNA e foram identificadas duas possíveis regiões codificadoras das proteínas de movimento e replicase viral. Baseado nestas regiões foram desenhados "primers" para amplificação do RNA do SvRSV por RT-PCR. Sondas baseadas nas seqüências obtidas hibridizaram com ss- e dsRNA de D. stramonium infectadas pelo vírus. Ensaios preliminares de RT-PCR e hibridização não resultaram em reação com o vírus da leprose dos citros, tipo citoplasmático (CiLV-C.Solanum violaefolium is an ornamental plant, with prostrate, trailing growth habit and is cultivated in shaded areas. A virus that causes

  19. Toxicity of plant essential oils to Tetranychus urticae (Acari: Tetranychidae) and Phytoseiulus persimilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Choi, Won-Il; Lee, Sang-Geui; Park, Hyung-Man; Ahn, Young-Joon

    2004-04-01

    Fifty-three plant essential oils were tested for their toxicity against eggs and adults of Tetranychus urticae Koch as well as adults of Phytoseiulus persimilis Athias-Henriot, by using a filter paper diffusion bioassay without allowing direct contact. Responses varied according to oil type and dose, and mite species. In a plastic container (4.5 by 9.5 cm) bioassay at 14 x 10(-3) microl/ml air, caraway seed, citronella java, lemon eucalyptus, pennyroyal, and peppermint oils gave > 90% mortality against adult T. urticae, whereas 82 and 81% mortality was observed with sage and spearmint oils, respectively. With the exception of sage oil, the other six essential oils were highly effective against T. urticae eggs at 9.3 x 10(-3) microl/ml air. Against adult P. persimilis, these six test oils caused > 90% mortality at 7.1 x 10(-3) microl/ml air. Particularly peppermint oil at 4.7 x 10(-3) microl/ml air was highly toxic. In an acrylic cage (30 by 30 by 40 cm ) test, lemon eucalyptus, pennyroyal, peppermint, and spearmint oils were highly effective against adult T. urticae at 1.4 x 10(-3) microl/ml air. These results indicate that the mode of delivery of these essential oils was largely a result of action in the vapor phase via the respiratory system. The essential oils described herein merit further study as potential fumigants for T. urticae control.

  20. Aedes communis Reactivity Is Associated with Bee Venom Hypersensitivity: An in vitro and in vivo Study.

    Science.gov (United States)

    Scala, Enrico; Pirrotta, Lia; Uasuf, Carina G; Mistrello, Gianni; Amato, Stefano; Guerra, Emma Cristina; Locanto, Maria; Meneguzzi, Giorgia; Giani, Mauro; Cecchi, Lorenzo; Abeni, Damiano; Asero, Riccardo

    2018-01-01

    Mosquito bite is usually followed by a local reaction, but severe or systemic reaction may, in rare cases, occur. Allergic reactions to Aedes communis (Ac) may be underestimated due to the lack of reliable diagnostic tools. In this multicenter study, 205 individuals reporting large local reactions to Ac were enrolled and studied for cutaneous or IgE reactivity to Ac, Blattella germanica, Penaeus monodon, and Dermatophagoides pteronyssinus. Extract and molecular IgE reactivity to bees, wasps, hornets, and yellow jacket venoms were also studied in 119 patients with a clinical history of adverse reaction to Hymenoptera. Immunoblot (IB) analysis and immunoCAP IgE inhibition experiments were carried out in selected sera. Ac sensitization was recorded in 96 (46.8%) patients on SPT. Strict relationship between Ac and D. pteronyssinus, B. germanica, P. monodon, or Apis mellifera reactivity on SPT was observed. Ac IgE recognition was seen in 60/131 (45.8%) patients, 49 (81.6%) of them SPT positive, and 5/14 IB reactors. Ac IgE sensitization was associated with Tabanus spp, A. mellifera, Vespula vulgaris, and Polistes dominula reactivity. A strict relationship between Ac IgE reactivity and Api m 1, Api m 2, Api m 3, Api m 5, and Api m 10 was recorded. IgE reactivity to AC was inhibited in 9/15 cases after serum absorption with the A. mellifera extract. Both SPT and IgE Ac reactivity is observed in about half of patients with a history of large local reactions to mosquito bites. The significant relationship between Ac sensitization and either extract or single bee venom components is suggestive of a "bee-mosquito syndrome" occurrence. © 2018 S. Karger AG, Basel.

  1. Effect of Age on Allergen Responses of Allergic Patients in Southern Taiwan

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    Wen Chiang

    2004-07-01

    Full Text Available To survey airborne and food allergen patterns in southern Taiwan and to analyze the effect of age on response to different allergens, we tested samples from 4,411 allergic patients at Kaohsiung Medical University Hospital using the MAST-CLA test (new Taiwan panel. A total of 2,212 (50.1% samples showed a positive response. We grouped allergic patients into five age groups. Milk and egg white were the main food allergens in the younger groups (< 3 years old and 3-6 years old. Shrimp, crab, and shellfish were the main allergens in the groups aged 7-12, 13-18, and more than 18 years. Among airborne allergens, house dust and mites Dermatophagoides farinae and D. pteronyssinus were the main allergens in all age groups, whereas the frequency of response to cockroach allergen was low in the group aged less than 3 years, but increased in the other age groups. There was a sharp increase in the frequency of response to airborne allergens after 3 years old and a sharp decrease in response to food allergens. Among subjects allergic to both airborne and food allergens, there was a positive MAST-CLA rate of 19.9% to 26% (all five age groups, no significant difference. When we compared our results with those from Taipei Veterans General Hospital in northern Taiwan, there were significant differences for yeast, peanut, feather mix, dog dander, cockroach, D. farinae and D. pteronyssinus allergens (p < 0.01. These differences were probably caused by differences in patient location, patient age, disease patterns and allergen panels.

  2. Effects of Helicobacter pylori, geohelminth infection and selected commensal bacteria on the risk of allergic disease and sensitization in 3-year-old Ethiopian children.

    Science.gov (United States)

    Amberbir, A; Medhin, G; Erku, W; Alem, A; Simms, R; Robinson, K; Fogarty, A; Britton, J; Venn, A; Davey, G

    2011-10-01

    Epidemiological studies have suggested that gastro-intestinal infections including Helicobacter pylori, intestinal microflora (commensal bacteria) and geohelminths may influence the risk of asthma and allergy but data from early life are lacking. We aimed to determine the independent effects of these infections on allergic disease symptoms and sensitization in an Ethiopian birth cohort. In 2008/09, 878 children (87% of the 1006 original singletons in a population-based birth cohort) were followed up at age 3 and interview data obtained on allergic symptoms and potential confounders. Allergen skin tests to Dermatophagoides pteronyssinus and cockroach were performed, levels of Der p 1 and Bla g 1 in the child's bedding measured and stool samples analysed for geohelminths and, in a random subsample, enterococci, lactobacilli, bifidobacteria and H. pylori antigen. The independent effects of each exposure on wheeze, eczema, hayfever and sensitization were determined using multiple logistic regression. Children were commonly infected with H. pylori (41%; 253/616), enterococci (38.1%; 207/544), lactobacilli (31.1%; 169/544) and bifidobacteria (18.9%; 103/544) whereas geohelminths were only found in 8.5% (75/866). H. pylori infection was associated with a borderline significant reduced risk of eczema (adjusted OR 0.49, 95% CI 0.24-1.01, P=0.05) and D. pteronyssinus sensitization (adjusted OR 0.42, 95% CI 0.17-1.08, P=0.07). Geohelminths and intestinal microflora were not significantly associated with any of the outcomes measured. Among young children in a developing country, we found evidence to support the hypothesis of a protective effect of H. pylori infection on the risk of allergic disease. Further investigation of the mechanism of this effect is therefore of potential therapeutic and preventive value. © 2011 Blackwell Publishing Ltd.

  3. Pesquisa de fungos em 20 pacientes com polipose nasossinusal Fungal research in 20 patientes with nasal polyposis

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    Carla R. Monteiro

    2002-10-01

    Full Text Available Introdução: Polipose nasossinusal é uma doença freqüente na prática clínica otorrinolaringológica. Sua associação com fungos, porém, tem recebido mais atenção apenas nos últimos 20 anos, principalmente após o reconhecimento da sinusite fúngica alérgica. Objetivo: Identificar a presença de fungos nos seios paranasais de pacientes portadores de polipose nasossinusal e analisar a reatividade destes pacientes para aeroalérgenos. Forma de Estudo: Clínico prospectivo. Material e Método: Avaliamos de forma prospectiva 20 pacientes com diagnóstico de polipose nasossinusal, que se apresentaram ao ambulatório de Otorrinolaringologia do Hospital Universitário Clementino Fraga Filho. Durante o tratamento cirúrgico, a secreção removida dos seios paranasais foi colocada em Sabouraud com cloranfenicol e submetida à análise histopatológica. Foram dosados níveis séricos de IgE total, IgE específica para Aspergillus fumigatus e contagem de eosinófilos do sangue periférico. Testes cutâneos com antígenos de Dermatophagoides pteronyssinus, Dermatophagoides farinae, Blomia tropicalis, Blattella germânica, Blattella americana e Aspergillus fumigatus foram realizados .Resultados: Seis pacientes (30% apresentaram crescimento fúngico, sendo quatro Aspergillus sp, um Candida tropicalis e outro Cladophialophora carrionii. Treze (68,42% apresentaram teste de puntura positivo para os aeroalérgenos acima citados. Em dez pacientes (50% foi encontrada eosinofilia. Níveis séricos elevados de IgE total foram encontrados em oito (44,4%. Nível sérico elevado de IgE específica para Aspergillus fumigatus foi encontrado em apenas um. Conclusão: Salientamos a importância da pesquisa de fungos e de uma investigação do sistema imune nos pacientes com polipose nasossinusal para ser feito um diagnóstico preciso e tratamento adequado.Introduction: Nasal polyposis is a frequent disease in the otolaryngology clinical practice. The association

  4. A novel and well tolerated mite allergoid subcutaneous immunotherapy: evidence of clinical and immunologic efficacy

    Science.gov (United States)

    Roger, Albert; Depreux, Nathalie; Jurgens, Yani; Heath, Matthew D; Garcia, Gloria; Skinner, Murray A

    2014-01-01

    Allergy to house dust mite is one of the most common causes of allergic rhinitis. A novel tyrosine-adsorbed, modified allergen product, Acarovac Plus, developed for the treatment of perennial mite allergy seeks to address the underlying cause of allergic rhinitis in this instance. One of two dosing regimens may be used, either the Conventional Regimen or the Cluster Regimen. We sought to compare the efficacy and safety of a specific immunotherapy, developed for the treatment of perennial mite allergy, administered under a Conventional and Clustered dosing schedule in patients with persistent allergic rhinitis. Thirty adult patients, between 18 and 65 years old, with allergic rhinitis and/or asthma secondary to hypersensitivity to Dermatophagoides pteronyssinus were administered with either conventional or cluster initial regime, with a final visit one week after the last dose administration. The efficacy to the Conventional and Cluster regimens was measured using a Nasal Challenge Test monitoring clinical symptoms and peak nasal inspiratory flow. Total IgE, serum-specific inmunoglobulins (IgE and IgG4) to Dermatophagoides pteronyssinus and relevant cytokines (IFN-γ, IL-4, IL-5, IL-10 and IL-13) were assessed. A Satisfaction Questionnaire (TSQM) was completed after each patient's final visit. The tolerability of the vaccine was assessed monitoring adverse reactions. No adverse events were recorded in either conventional or cluster regime. The specific Nasal Challenge Test led to a decrease in symptom scores and a significant decrease in mean nasal peak inspiratory flow drop was recorded in both dosing regimen groups. A significant increase in IgG4-specific antibody titres was assessed. No significant changes were observed in concentrations of total IgE, specific IgE or cytokines (IFN-γ, IL-4, IL-5, IL-10 and IL-13). Patients declared themselves most satisfied in relation to “Secondary effects”, with high overall satisfaction in both groups. Cluster and

  5. Xiao-Qing-Long-Tang shows preventive effect of asthma in an allergic asthma mouse model through neurotrophin regulation

    Science.gov (United States)

    2013-01-01

    Background This study investigates the effect of Xiao-Qing-Long-Tang (XQLT) on neurotrophin in an established mouse model of Dermatophagoides pteronyssinus (Der p)-induced acute allergic asthma and in a LA4 cell line model of lung adenoma. The effects of XQLT on the regulation of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), airway hyper-responsiveness (AHR) and immunoglobulin E were measured. Methods LA4 cells were stimulated with 100 μg/ml Der p 24 h and the supernatant was collected for ELISA analysis. Der p-stimulated LA4 cells with either XQLT pre-treatment or XQLT co-treatment were used to evaluate the XQLT effect on neurotrophin. Balb/c mice were sensitized on days 0 and 7 with a base-tail injection of 50 μg Dermatophagoides pteronyssinus (Der p) that was emulsified in 50 μl incomplete Freund’s adjuvant (IFA). On day 14, mice received an intra-tracheal challenge of 50 μl Der p (2 mg/ml). XQLT (1g/Kg) was administered orally to mice either on days 2, 4, 6, 8, 10 and 12 as a preventive strategy or on day 15 as a therapeutic strategy. Results XQLT inhibited expression of those NGF, BDNF and thymus-and activation-regulated cytokine (TARC) in LA4 cells that were subjected to a Der p allergen. Both preventive and therapeutic treatments with XQLT in mice reduced AHR. Preventive treatment with XQLT markedly decreased NGF in broncho-alveolar lavage fluids (BALF) and BDNF in serum, whereas therapeutic treatment reduced only serum BDNF level. The reduced NGF levels corresponded to a decrease in AHR by XQLT treatment. Reduced BALF NGF and TARC and serum BDNF levels may have been responsible for decreased eosinophil infiltration into lung tissue. Immunohistochemistry showed that p75NTR and TrkA levels were reduced in the lungs of mice under both XQLT treatment protocols, and this reduction may have been correlated with the prevention of the asthmatic reaction by XQLT. Conclusion XQLT alleviated allergic inflammation including AHR, Ig

  6. Effectiveness of house dust mite acaricide tri-n-butyl tin maleate on carpets, fabrics and mattress foam: a standardization of methodology Eficácia do acaricida maleato de estanho tri-n-butílico contra ácaros de poeira em carpetes, tecidos e espuma de colchão: padronização de metodologia

    Directory of Open Access Journals (Sweden)

    Satiko Uehara

    2006-06-01

    Full Text Available The aim of this study was to determine the effectiveness of the acaricide tri-n-butyl tin maleate, industrially applied to samples of carpets, mattress foam, and fabrics used for furniture upholstery, soft toys and shoe uppers. Approximately 100 adult house dust mites of the species Dermatophagoides pteronyssinus were inoculated into a Petri dish containing the sample (a piece of carpet, mattress foam, or fabric, treated with the acaricide, randomly collected. Mite-maintenance culture medium was added on top of each sample. After one, two, three, seven and 30 days of incubation at 25 ºC and 75% relative humidity, each dish was examined using a 40X stereoscopic microscope (40X. One hundred percent acaricide effectiveness was obtained in treated materials by the end of the 30th-day postinoculation period, under optimal conditions for mite maintenance.O objetivo do presente estudo foi verificar a eficácia do acaricida maleato de estanho tri-n-butílico, aplicado industrialmente em amostras de carpetes, tecidos de revestimentos de móveis e de calçados, assim como de espumas de colchão. Aproximadamente 100 ácaros adultos da espécie Dermatophagoides pteronyssinus foram inoculados em placa de Petri contendo a amostra (pedaço de colchão, tecido ou carpete, tratada com o produto acaricida, coletados aleatoriamente. Foi acrescentado sobre a amostra, meio de cultivo para a manutenção dos ácaros. Cada placa foi examinada após 1, 2, 3, 7 e 30 dias de incubação a 25 ºC e 75% de U.R.A. (umidade relativa do ar, sob microscópio estereoscópico com 40X de aumento. O acaricida maleato de estanho tri-n-butílico apresentou 100% de eficácia acaricida após 30 dias da aplicação, em condições ótimas para a manutenção dos ácaros.

  7. Association between pet ownership and the sensitization to pet allergens in adults with various allergic diseases.

    Science.gov (United States)

    Park, Yong-Bum; Mo, Eun-Kyung; Lee, Jae-Young; Kim, Joo-Hee; Kim, Cheol-Hong; Hyun, In-Gyu; Choi, Jeong-Hee

    2013-09-01

    As pet ownership increases, sensitization to animal allergens due to domestic exposure is a concern. Sensitization to animal allergens may occur from indirect exposure, as well as direct ownership of animals. However, there have been conflicting results regarding the association between pet ownership and sensitization to animal allergens in adults. In total, 401 patients with various allergic diseases were enrolled in this study. We performed skin prick tests with 55 common inhalant and food allergens, including dog, cat, and rabbit allergens. A mean wheal diameter of 3 mm or greater was considered a positive reaction. The exposure modality to each animal allergen was investigated using a questionnaire and included present ownership, past ownership, occupational exposure, occasional exposure, contact with pet owner, and no contact. Present ownership, past ownership, occupational, and occasional exposure were regarded as direct exposure. The sensitization rate for animal allergens was 20.4% for dog, 15.0% for cat, and 9.0% for rabbit. Direct exposure to dogs (72.0%) was significantly higher than that of other animals (18.4% for cats and 16.7% for rabbits), whereas 'no contact' with cats (78.3%) and rabbits (83.3%) was significantly higher than with dogs (26.8%; Prisk factors for sensitization to animal allergens were sensitization to Dermatophagoides pteronyssinus (OR=2.4, P=0.052), Dermatophagoides farinae (OR=5.1, Prisk factor was sensitization to Alternaria (OR=6.0, P<0.002). These results suggest that direct exposure to dogs contributes to the sensitization to dog allergens in patients with allergic diseases, whereas indirect exposure to cats and rabbits may induce sensitization to each animal's allergen.

  8. [Profile of sensitization to allergens in children with atopic dermatitis assisting to Allergology Service of University Hospital, Nuevo Leon, Mexico].

    Science.gov (United States)

    Yong-Rodríguez, Adrián; Macías-Weinmann, Alejandra; Palma-Gómez, Samuel; Arias-Cruz, Alfredo; Pérez-Vanzzini, Rafael; Gutiérrez-Mujica, José Julio; González-Díaz, Sandra Nora

    2015-01-01

    Sensitization to allergens in atopic dermatitis patients is a risk factor for developing asthma and allergic rhinitis in the future,as well as an aggravating factor in the course of the disease. Recent studies have attributed the activity of the proteases of some antigens to cause a grater defect in the epithelial barrier and a more severe disease. To know the sensitization to allergens pattern in children with atopic dermatitis attended at Allergology Service of University Hospital of UANL, Mexico, and to know if these children have higher sensitization to antigens with proteolytic activity. A retrospective study was done reviewing the skin prick test reports done in our service to children ranging from 5 months to 16 years old, diagnosed with atopic dermatitis during a period of 2 years, from January 2012 to January 2014. The frequency of sensitization to aeroallergens and food were analyzed as well as the weal size (≥6mm) on the skin in response to each particular allergen in the case of food skin prick test. Reports of skin tests of 66 children, 30 boys and 36 girls, were included; 37 of children were sensitized to more than one allergen,18/66 had asthma and/or allergic rhinitis, 40/66 60% skin prick tests were positive to high activity protease aeroallergens (Dermatophagoides pteronyssinus/Dermatophagoides farinae). Regarding food, sensitization was seen in 38 children; fruits and vegetables were the two most common foods. Only seven children had skin prick weal bigger than 6 mm, mainly to egg, fish and cow's milk. Children with atopic dermatitis are often sensitized to high protease activity aeroallergens, polysensitization is very common and the association with airway allergy is seen early in life. Sensitization to food is also common in these patients, but only a small percentage showed a response large enough to be associated with disease severity.

  9. Structural Analysis of Der p 1–Antibody Complexes and Comparison with Complexes of Proteins or Peptides with Monoclonal Antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Osinski, Tomasz; Pomés, Anna; Majorek, Karolina A.; Glesner, Jill; Offermann, Lesa R.; Vailes, Lisa D.; Chapman, Martin D.; Minor, Wladek; Chruszcz, Maksymilian (INDOOR); (UV); (SC)

    2015-05-29

    Der p 1 is a major allergen from the house dust mite, Dermatophagoides pteronyssinus, that belongs to the papain-like cysteine protease family. To investigate the antigenic determinants of Der p 1, we determined two crystal structures of Der p 1 in complex with the Fab fragments of mAbs 5H8 or 10B9. Epitopes for these two Der p 1–specific Abs are located in different, nonoverlapping parts of the Der p 1 molecule. Nevertheless, surface area and identity of the amino acid residues involved in hydrogen bonds between allergen and Ab are similar. The epitope for mAb 10B9 only showed a partial overlap with the previously reported epitope for mAb 4C1, a cross-reactive mAb that binds Der p 1 and its homolog Der f 1 from Dermatophagoides farinae. Upon binding to Der p 1, the Fab fragment of mAb 10B9 was found to form a very rare α helix in its third CDR of the H chain. To provide an overview of the surface properties of the interfaces formed by the complexes of Der p 1–10B9 and Der p 1–5H8, along with the complexes of 4C1 with Der p 1 and Der f 1, a broad analysis of the surfaces and hydrogen bonds of all complexes of Fab–protein or Fab–peptide was performed. This work provides detailed insight into the cross-reactive and specific allergen–Ab interactions in group 1 mite allergens. The surface data of Fab–protein and Fab–peptide interfaces can be used in the design of conformational epitopes with reduced Ab binding for immunotherapy.

  10. 11 Efficacy and Tolerability of HDM Injective Immunotherapy With Monomeric Allergoid

    Science.gov (United States)

    Compalati, Enrico; Atzeni, Isabella; Cabras, Sergio; Fancello, Paolo; Gaspardini, Giulio; Longo, Rocco; Patella, Vincenzo; Tore, Giorgio

    2012-01-01

    Background Subcutaneous immunotherapy (SCIT) is an effective treatment of respiratory allergy and carbamylated monomeric allergoids (monoids), by virtue of their reduced IgE-binding activity, resulted clinically safe by sublingual administration. Purpose of this study was to investigate the efficacy and tolerability of immunotherapy with house dust mites (HDM) monoid administered by injective route in patients with allergic rhinoconjunctivitis (AR). Methods A preparation of 0.70 mL of 10 BU/mL containing modified extract with 50% Dermatophagoides pteronyssinus and 50% Dermatophagoides farinae (amount of major allergen: 4 μg of group 1 per milliliter) was delivered monthly for 12 months, following a 5-week build-up induction phase (0.10–0.20–0.30–0.50–0.70 mL), to 58 patients (60% males, mean age 25.1 ± 12.7) suffering from AR due to mites for at least 2 years, whereas 60 patients with similar baseline characteristics were observed as controls. All patients were allowed to assume traditional drug therapy for their condition. At the end of the study changes from baseline in symptoms scores, in number of days with drug assumption, in severity of AR (according to ARIA classification) were compared between the 2 groups; moreover an overall assessment of clinical efficacy and tolerability was based on patients' and physicians' judgements (unsatisfactory, mild, good, optimal). Results In respect to baseline both groups showed, after 1 year, an improvement in symptoms score (P allergoid was associated with a significant clinical benefit observed through objective and subjective outcomes; the traditional safety of monomeric allergoids was confirmed by the subjective judgements of tolerability.

  11. Influence of environmental characteristics and climatic factors on mites in the dust of air-conditioner filters.

    Science.gov (United States)

    Wu, J; Liu, Z G; Ran, P X; Wang, B

    2009-12-01

    To investigate mites in the dust of air-conditioner filters (MACF) in China, a total of 652 dust samples were collected from six cities: Guangzhou (n = 129), Nanchang (n = 127), Shanghai (n = 113), Xian (n = 93), Beijing (n = 93), and Shenyang (n = 79). Tarsonemus granarius was the most dominant species (87.2%). Dermatophagoides pteronyssinus and Dermatophagoides farinae only represented 7.0 and 3.0% of total mites, respectively. With latitude increasing, both mite occurrence rate (P air-conditioner age, utilization time and power. Wall and window type air-conditioner had higher risk of finding MACF than the floor type air-conditioner. As far as the cleaning interval time of ACF was concerned, higher risk ratio and the highest density of MACF were found in the time stage of >3, air-conditioner filters are potential indoor threat to asthma and allergy sufferers. In this study, we find that the storage mite Tarsonemus granarius is the predominant species of mites in the dust of air-conditioner filters (MACF). Thus, the possible clinical importance of T. granarius should cause more our attentions in the future. The abundance and distribution of MACF are also found significantly varied in different climatic regions of China. When we try to assess the possible risk of MACF, more attentions should be focused on subtropical region than temperate region. The influence analysis of environmental characteristics on the prevalence of MACF will shed light on the establishment of mite control strategy and the design of mite defense air-conditioner.

  12. European symposium on the awareness of allergy: report of the promotional campaign in the European Parliament (26-28 April 2016).

    Science.gov (United States)

    Muraro, A; Steelant, B; Pietikainen, S; Borrelli, D; Childers, N; Callebaut, I; Kortekaas Krohn, I; Martens, K; Pugin, B; Popescu, F-D; Vieru, M; Jutel, M; Agache, I; Hellings, P W

    2017-02-01

    From 26 to 28 of April 2016, an allergy awareness campaign was organized by the European Academy of Allergy and Clinical Immunology and the European Federation of Allergy and Airway Diseases Patients Associations in the European Parliament in Brussels, with support of the European Parliament's Interest group on Allergy and Asthma and was co-hosted by the Members of the European Parliament David Borrelli, Sirpa Pietikainen and Nessa Childers. Skin prick tests (SPTs) were performed to gain attention for the increasing prevalence of allergic airway diseases in Europe. Since more than 30% of the total European population suffers from airway allergies and asthma, reaching a higher level of awareness and elaboration of an active prevention plan is mandatory. Of the 406 individuals undergoing SPT in the European Parliament, 211 participants (52%) reported to have suffered from an allergy in the past, with allergic symptoms being present in the nose and eyes (40% and 36%, respectively), the skin (27%), lower airways (14%) and the gut (8%). Of the 381 SPT with reliable results, cutaneous hypersensitivity was found in 201 (53%) participants. Of those with positive SPT (n = 201), 70 participants (35%) were monosensitized while 131 participants (65%) were polysensitized. The positive skin reactions were found mostly for grass pollen (n = 108), followed by Dermatophagoides pteronyssinus (n = 105), Dermatophagoides farina (n = 96) and birch pollen (n = 85). Of note, 54 individuals (14% of the total tested population) without reported allergy or allergic symptoms showed a positive SPT without clinical relevance. This report summarizes the main idea and goals of the symposium: chronic airway diseases are a major and growing health problem in Europe. Therefore, a joint preventive action plan needs to be developed for a better health status of European citizens. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  13. The prevalence of positive reactions in the atopy patch test with aeroallergens and food allergens in subjects with atopic eczema: a European multicenter study.

    Science.gov (United States)

    Darsow, U; Laifaoui, J; Kerschenlohr, K; Wollenberg, A; Przybilla, B; Wüthrich, B; Borelli, S; Giusti, F; Seidenari, S; Drzimalla, K; Simon, D; Disch, R; Borelli, S; Devillers, A C A; Oranje, A P; De Raeve, L; Hachem, J-P; Dangoisse, C; Blondeel, A; Song, M; Breuer, K; Wulf, A; Werfel, T; Roul, S; Taieb, A; Bolhaar, S; Bruijnzeel-Koomen, C; Brönnimann, M; Braathen, L R; Didierlaurent, A; André, C; Ring, J

    2004-12-01

    The atopy patch test (APT) was proposed to evaluate IgE-mediated sensitizations in patients with atopic eczema (AE). The prevalence and agreement with clinical history and specific IgE (sIgE) of positive APT reactions was investigated in six European countries using a standardized method. A total of 314 patients with AE in remission were tested in 12 study centers on clinically uninvolved, non-abraded back skin with 200 index of reactivity (IR)/g of house dust mite Dermatophagoides pteronyssinus, cat dander, grass, and birch pollen allergen extracts with defined major allergen contents in petrolatum. Extracts of egg white, celery and wheat flour with defined protein content were also patch tested. APT values were evaluated at 24, 48, and 72 h according to the European Task Force on Atopic Dermatitis (ETFAD) guidelines. In addition, skin-prick test (SPT) and sIgE and a detailed history on allergen-induced eczema flares were obtained. Previous eczema flares, after contact with specific allergens, were reported in 1% (celery) to 34% (D. pteronyssinus) of patients. The frequency of clear-cut positive APT reactions ranged from 39% with D. pteronyssinus to 9% with celery. All ETFAD intensities occured after 48 and 72 h. Positive SPT (16-57%) and elevated sIgE (19-59%) results were more frequent. Clear-cut positive APT with all SPT and sIgE testing negative was seen in 7% of the patients, whereas a positive APT without SPT or sIgE for the respective allergen was seen in 17% of the patients. APT, SPT and sIgE results showed significant agreement with history for grass pollen and egg white (two-sided Pr > /Z/ atopic controls, no positive APT reaction was seen. Aeroallergens and food allergens are able to elicit eczematous skin reactions after epicutaneous application. As no gold standard for aeroallergen provocation in AE exists, the relevance of aeroallergens for AE flares may be evaluated by APT in addition to SPT and sIgE. The data may contribute to the international

  14. Measurement of IgG antibodies to house dust mite and grass pollen by a solid-phase radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Delespesse, G; Debisschop, M J; Flament, J [Hopital Saint Pierre, Louvain (Belgium). Lab. de Recherches de la Clinique Medicale

    1979-09-01

    A solid-phase radioimmunoassay was used to measure specific IgG antibodies to either Dermatophagoides pteronyssinus (DPT) or grass pollens. Radiolabelled protein A from Staphylococcus aureus (SpA) was used to determine the IgG antibodies attached to the microtubes. The binding of IgG from either normal or allergic sera to DPT-coated tubes was antigen specific and mediated by the Fab fragment of the immunoglobulin. IgG antibodies from non-allergic serum competed with IgE antibodies to DPT. IgE antibodies did not significantly interfere with the assay. Indeed heating a reaginic serum resulted in a striking reduction of the (/sup 125/I) anti- IgE binding to allergen-coated tubes without modifying the (/sup 125/I)-SpA binding. Furthermore, filtration of a reaginic serum through Sephacryl S-200 separated a peak of IgE antibodies. The solid phase method was more sensitive than a double-antibody technique employing the same DPT extract as labelled antigen. Non-allergic subjects had less IgG antibodies to DPT or grass pollens than allergic patients. In untreated patients, there was a good correlation between levels of IgG and IgE antibodies to grass pollens but not to DPT. Patients hyposensitized to house dust mite had on the average three times more specific IgG antibodies than untreated cases. (author).

  15. Comparative efficacy of house dust mite extermination products.

    Science.gov (United States)

    Schober, G; Kniest, F M; Kort, H S; De Saint Georges Gridelet, D M; Van Bronswijk, J E

    1992-06-01

    The acaricidal efficacy of nine marketed products, i.e. Acardust, Acarosan (foam and powder), Actelic 50, Artilin 3A (spirit and water base), liquid nitrogen, Paragerm AK, and Tymasil, and of intensive vacuum-cleaning have been compared on four different test surfaces: mattress, tufted carpet, gypsum board and rough wooden board, all covered with artificial house dust. They were inoculated with the house dust mite, Dermatophagoides pteronyssinus or the house-dust fungus Aspergillus repens for evaluation of the fungistatic claims of some products. The acaricidal activity of Tymasil did not surpass that of vacuuming; its fungistatic effect was not apparent. The other products showed complete to almost complete eradication on at least one of the substrates tested. Taking into account the results of acaricidal efficacy as well as the data on safety and practicality acquired earlier, Acarosan powder was considered first choice for carpet treatment. Acarosan and liquid nitrogen, were found to be effective in the treatment of mattress, pillow, upholstered furniture and heavy curtains. On wooden surfaces Acarosan was found to be both effective and safe, while Acardust, Actelic 50, Artilin 3A (both fungistatic as well as acaricidal), liquid nitrogen and Paragerm also passed the efficiency test.

  16. Mite fauna and fungal flora in house dust from homes of asthmatic children.

    Science.gov (United States)

    Ishii, A; Takaoka, M; Ichinoe, M; Kabasawa, Y; Ouchi, T

    1979-12-01

    Mite fauna and fungal flora in the house dust from homes of asthmatic children with positive and negative skin test to house dust allergen and non-asthmatic controls were examined. There was no conspicuous difference in mite species distribution among the three groups. Pyroglyphid mites dominate the mite fauna in house dust more than half of which being Dermatophagoides: D. pteronyssinus and D. farinae. There was no statistically significant difference in numbers between the two species and either species could dominate depending on the conditions of the individual houses. The average number of acarina in 0.5 g of fine dust did not differ statistically among the three groups; however, mite number per square meter floor differed between patients with positive skin test and negative skin test. The results suggest that house-cleaning might influence the possible sensitization of children. The genetic distribution of mould fungi in house dust was largely similar to that of airborne fungi. The average number of fungal colonies detected in 0.5 g of dust did not differ statistically among the three groups. Wallemia with its minute spores may cause sensitization but has so far been insufficiently investigated.

  17. Patch test reactions to mite antigens: a GERDA multicentre study. Groupe d'Etudes et de Recherches en Dermato-Allergie.

    Science.gov (United States)

    Castelain, M; Birnbaum, J; Castelain, P Y; Ducombs, G; Grosshans, E; Jelen, G; Lacroix, M; Meynadier, J; Mougeolle, J M; Lachapelle, J M

    1993-11-01

    We performed patch tests with Dermatophagoides pteronyssinus (Dp) antigens from 2 different sources in 355 non-randomly selected patients with atopic dermatitis (AD) and 398 subjects of a control group. The study demonstrated that contact sensitization to mites occurred in an appreciable % of AD cases (20.8%), using commonly available assay products. The differences recorded between the 2 materials tested were related to the concentration of P1 antigen. Non-atopic patients rarely showed positive reactions to Dp (0.75%), when strict criteria for readings were applied and if 2 readings were performed. Patients with positive patch tests did not necessarily show positive immediate skin tests. It would be useful to carry out tests systematically in atopic patients, even if it is not yet known what modern treatment would be best for the patient. Laboratories still do not provide standardized house dust mite preparations--measuring and codifying their biological activity--for use in patch tests. It is to be hoped that the extension of this type of test will lead to the production of better test materials, in syringes with homogeneous dispersion and concentration.

  18. Stability evaluation of house dust mite vaccines for sublingual immunotherapy

    Directory of Open Access Journals (Sweden)

    MARIJA GAVROVIĆ-JANKULOVIĆ

    2010-01-01

    Full Text Available Allergen-specific immunotherapy with house dust mite (HDM allergen extracts can effectively alleviate the symptoms of allergic rhinitis and asthma. The efficacy of the immunotherapeutic treatment is highly dependent on the quality of house dust mite vaccines. This study was performed to assess the stability of house dust mite allergen vaccines prepared for sublingual immunotherapy. Lyophilized Dermatophagoides pteronyssinus (Dpt mite bodies were the starting material for the production of sublingual vaccines in four therapeutic concentrations. The stability of the extract for vaccine production, which was stored below 4 °C for one month, showed consistence in the protein profile in SDS PAGE. ELISA-inhibition showed that the potencies of Dpt vaccines during a 12 month period were to 65–80 % preserved at all analyzed therapeutic concentrations. This study showed that glycerinated Dpt vaccines stored at 4 °C preserved their IgE-binding potential during a 12 month period, implying their suitability for sublingual immunotherapeutic treatment of HDM allergy.

  19. IMD-4690, a novel specific inhibitor for plasminogen activator inhibitor-1, reduces allergic airway remodeling in a mouse model of chronic asthma via regulating angiogenesis and remodeling-related mediators.

    Directory of Open Access Journals (Sweden)

    Toshifumi Tezuka

    Full Text Available Plasminogen activator inhibitor (PAI-1 is the principal inhibitor of plasminogen activators, and is responsible for the degradation of fibrin and extracellular matrix. IMD-4690 is a newly synthesized inhibitor for PAI-1, whereas the effect on allergic airway inflammation and remodeling is still unclear. We examined the in vivo effects by using a chronic allergen exposure model of bronchial asthma in mice. The model was generated by an immune challenge for 8 weeks with house dust mite antigen, Dermatophagoides pteronyssinus (Dp. IMD-4690 was intraperitoneally administered during the challenge. Lung histopathology, hyperresponsiveness and the concentrations of mediators in lung homogenates were analyzed. The amount of active PAI-1 in the lungs was increased in mice treated with Dp. Administration with IMD-4690 reduced an active/total PAI-1 ratio. IMD-4690 also reduced the number of bronchial eosinophils in accordance with the decreased expressions of Th2 cytokines in the lung homogenates. Airway remodeling was inhibited by reducing subepithelial collagen deposition, smooth muscle hypertrophy, and angiogenesis. The effects of IMD-4690 were partly mediated by the regulation of TGF-β, HGF and matrix metalloproteinase. These results suggest that PAI-1 plays crucial roles in airway inflammation and remodeling, and IMD-4690, a specific PAI-1 inhibitor, may have therapeutic potential for patients with refractory asthma due to airway remodeling.

  20. IMD-4690, a novel specific inhibitor for plasminogen activator inhibitor-1, reduces allergic airway remodeling in a mouse model of chronic asthma via regulating angiogenesis and remodeling-related mediators.

    Science.gov (United States)

    Tezuka, Toshifumi; Ogawa, Hirohisa; Azuma, Masahiko; Goto, Hisatsugu; Uehara, Hisanori; Aono, Yoshinori; Hanibuchi, Masaki; Yamaguchi, Yoichi; Fujikawa, Tomoyuki; Itai, Akiko; Nishioka, Yasuhiko

    2015-01-01

    Plasminogen activator inhibitor (PAI)-1 is the principal inhibitor of plasminogen activators, and is responsible for the degradation of fibrin and extracellular matrix. IMD-4690 is a newly synthesized inhibitor for PAI-1, whereas the effect on allergic airway inflammation and remodeling is still unclear. We examined the in vivo effects by using a chronic allergen exposure model of bronchial asthma in mice. The model was generated by an immune challenge for 8 weeks with house dust mite antigen, Dermatophagoides pteronyssinus (Dp). IMD-4690 was intraperitoneally administered during the challenge. Lung histopathology, hyperresponsiveness and the concentrations of mediators in lung homogenates were analyzed. The amount of active PAI-1 in the lungs was increased in mice treated with Dp. Administration with IMD-4690 reduced an active/total PAI-1 ratio. IMD-4690 also reduced the number of bronchial eosinophils in accordance with the decreased expressions of Th2 cytokines in the lung homogenates. Airway remodeling was inhibited by reducing subepithelial collagen deposition, smooth muscle hypertrophy, and angiogenesis. The effects of IMD-4690 were partly mediated by the regulation of TGF-β, HGF and matrix metalloproteinase. These results suggest that PAI-1 plays crucial roles in airway inflammation and remodeling, and IMD-4690, a specific PAI-1 inhibitor, may have therapeutic potential for patients with refractory asthma due to airway remodeling.

  1. Measurement of IgG antibodies to house dust mite and grass pollen by a solid-phase radioimmunoassay

    International Nuclear Information System (INIS)

    Delespesse, G.; Debisschop, M.J.; Flament, J.

    1979-01-01

    A solid-phase radioimmunoassay was used to measure specific IgG antibodies to either Dermatophagoides pteronyssinus (DPT) or grass pollens. Radiolabelled protein A from Staphylococcus aureus (SpA) was used to determine the IgG antibodies attached to the microtubes. The binding of IgG from either normal or allergic sera to DPT-coated tubes was antigen specific and mediated by the Fab fragment of the immunoglobulin. IgG antibodies from non-allergic serum competed with IgE antibodies to DPT. IgE antibodies did not significantly interfere with the assay. Indeed heating a reaginic serum resulted in a striking reduction of the ( 125 I) anti- IgE binding to allergen-coated tubes without modifying the ( 125 I)-SpA binding. Furthermore, filtration of a reaginic serum through Sephacryl S-200 separated a peak of IgE antibodies. The solid phase method was more sensitive than a double-antibody technique employing the same DPT extract as labelled antigen. Non-allergic subjects had less IgG antibodies to DPT or grass pollens than allergic patients. In untreated patients, there was a good correlation between levels of IgG and IgE antibodies to grass pollens but not to DPT. Patients hyposensitized to house dust mite had on the average three times more specific IgG antibodies than untreated cases. (author)

  2. The Effect of Serine Protease Inhibitors on Airway Inflammation in a Chronic Allergen-Induced Asthma Mouse Model

    Directory of Open Access Journals (Sweden)

    Chih-Che Lin

    2014-01-01

    Full Text Available Serine protease inhibitors reportedly attenuated airway inflammation and had antioxidant in multiorgan. However, the effects of the serine protease inhibitors nafamostat mesilate (FUT, gabexate mesilate (FOY, and ulinastatin (UTI on a long-term challenged mouse model of chronic asthma are unclear. BALB/c mice (6 mice/group were intratracheally inoculated with five doses of Dermatophagoides pteronyssinus (Der p; 50 μL, 1 mg/mL at one-week intervals. Therapeutic doses of FUT (0.0625 mg/kg, FOY (20 mg/kg, or UTI (10,000 U/kg were, respectively, injected intraperitoneally into these mice. Control mice received sterile PBS. At 3 days after the last challenge, mice were sacrificed to assess airway hyperresponsiveness (AHR, remodeling, and inflammation; lung histological features; and cytokine expression profiles. Compared with untreated controls, mice treated with FUT, FOY, and UTI had decreased AHR and goblet cell hyperplasia, decreased eosinophil and neutrophil infiltration, decreased Der p-induced IL-4 levels in serum and IL-5, IL-6, IL-13, and IL-17 levels in bronchoalveolar lavage fluid, and inhibited nuclear factor (NF-κB activity in lung tissues. The serine protease inhibitors FUT, FOY, and UTI have potential therapeutic benefits for treating asthma by downregulating Th2 cytokines and Th17 cell function and inhibiting NF-κB activation in lung tissue.

  3. The distribution of dust mite allergen in the houses of patients with asthma

    International Nuclear Information System (INIS)

    Tovey, E.R.; Chapman, M.D.; Wells, C.W.; Platts-Mills, T.A.

    1981-01-01

    Using an inhibition radioimmunoassay for the major allergen from Dermatophagoides pteronyssinus (antigen P1), we studied the distribution of this dust allergen in the houses of patients with asthma. Both bed and floor dust samples contained a wide range of antigen P1, 100 to 100,000 ng/g of fine dust, and this concentration correlated well with the number of mite bodies (r . 0.81, p less than 0.001). We were unable to detect antigen P1 in the air of undisturbed rooms. However, during domestic activity, between 1 and 30 ng were collected on a filter than sampled air for 45 min at 17 L/min. Using a cascade impactor it was shown that greater than 80% of the airborne antigen P1 was associated with particles greater than 10 mu in diameter. Some of the particles containing allergen could be identified because they formed precipitin rings when impacted onto agarose containing rabbit antimite antiserum. These particles had the physical appearance of mite feces, which are the major source of antigen P1 in mite cultures. The results suggested that natural exposure to this dust allergen allows occasional fecal particles to enter the lungs and that these particles contain very concentrated allergen

  4. Epicutaneous aeroallergen sensitization in atopic dermatitis infants - determining the role of epidermal barrier impairment.

    Science.gov (United States)

    Boralevi, F; Hubiche, T; Léauté-Labrèze, C; Saubusse, E; Fayon, M; Roul, S; Maurice-Tison, S; Taïeb, A

    2008-02-01

    Sensitization to atopens is an early phenomenon that overlaps with the onset of atopic dermatitis (AD) in infancy. Early epidermal barrier impairment may facilitate the epicutaneous penetration of atopens. To correlate transepidermal water loss (TEWL) and aeroallergen sensitization in infants with AD. In this cross-sectional study we enrolled 59 AD children and 30 controls aged 3-12 months. Transepidermal water loss in uninvolved skin, specific immunoglobulin E, atopy patch test (APT) and skin prick tests were performed with respect to seven aeroallergens, i.e., Dermatophagoides pteronyssinus, D. farinae, cat, dog, birch pollen, ambrosia, and cockroach. Environmental conditions were assessed by a questionnaire, and the house dust mite (HDM) concentration was determined in dust samples. Eighty-nine percent of AD infants had a positive APT vs one out of eleven controls. AD infants had a significantly higher mean TEWL than controls (27.4 vs 11.1 g/m(2)/h, P cats, and dogs at home. This study confirms the high prevalence of delayed sensitization to indoor and outdoor aeroallergens in AD infants, and shows that the higher the TEWL, the higher the prevalence of sensitization to aeroallergens. These data are in favor of a major role of a constitutive epidermal barrier impairment in determining early atopen sensitization in infants with AD.

  5. Atopy patch tests in young adult patients with atopic dermatitis and controls: dose-response relationship, objective reading, reproducibility and clinical interpretation.

    Science.gov (United States)

    Bygum, Anette; Mortz, Charlotte Gotthard; Andersen, Klaus Ejner

    2003-01-01

    The clinical interpretation and reproducibility of atopy patch tests was studied in 23 selected young adult patients with atopic dermatitis and 25 healthy controls using standard inhalant allergens. Non-invasive measurements were used for objective assessment of test reactions and the participants were retested after 6 weeks. Ten of 19 (53%) evaluable patients with atopic dermatitis had at least one positive atopy patch test. However, there was no clear clinical relevance of the atopy patch test results when related to patient history and distribution of dermatitis. Reproducible and dose-dependent results were obtained with Dermatophagoides pteronyssinus, grass and cat with a reproducibility rate of 0.69 to 0.81 in patients and 0.60-0.96 in controls. A unique finding was a significant positive correlation between a positive atopy patch test, allergen dose and increase in transepidermal water loss and erythema, while measurement of capacitance did not distinguish between positive and negative reactions. The results of the present study do not support the routine use of atopy patch tests in the evaluation of adult patients with atopic dermatitis.

  6. Allergen sensitisation among chronic respiratory diseases in urban and rural areas of the south of Viet Nam.

    Science.gov (United States)

    Chu, H T; Godin, I; Phuong, N T; Nguyen, L H; Hiep, T T M; Michel, O

    2018-02-01

    To evaluate the prevalence of and risk factors for allergen sensitisation among patients with chronic respiratory disease (CRD) in southern Viet Nam. An environmental questionnaire and skin prick tests for airborne and food allergens were administered to patients with CRD, defined as individuals with respiratory symptoms and lung function defects. Of 610 CRD patients, 56% had chronic obstructive pulmonary disease and 31% were asthma patients; 80% were males. The most frequent sensitisers were dust mites (Dermatophagoides farinae 22%, Blomia tropicalis 19%, D. pteronyssinus 18%) and cockroach droppings (13%). Among study participants, 37% were from rural settings and 36% from urban areas, whereas 27% had migrated from rural to urban areas. Compared with people from rural areas, being born in an urban area was a risk factor for sensitisation to mites (OR 1.56, 95%CI 1.11-2.20, P Viet Nam. Compared with the urban population, being native to a rural area was protective against mite sensitisation, but this effect ceased to be significant after migration from rural to urban areas.

  7. Modulation of the allergen-induced human IgE response in Hu-SCID mice: inhibitory effect of human recombinant IFN-gamma and allergen-derived lipopeptide.

    Science.gov (United States)

    Duez, C; Gras-Masse, H; Hammad, H; Akoum, H; Didierlaurent, A; André, C; Tonnel, A B; Pestel, J

    2001-01-01

    We have previously established a model to study the in vivo human IgE response using humanized SCID mice. Allergic SCID mice were obtained following intraperitoneal injection with mononuclear cells from Dermatophagoides pteronyssinus (Dpt)-sensitive patients, and sensitization by Dpt allergen intraperitoneal injection (immunization) or Dpt aerosol (inhalation). Human serum IgE was measured in allergic SCID mice after administration of human recombinant IFN-gamma or the lipopeptide LP 52-71 (derived from peptide p52-71 from Der p 1, Dpt major allergen, coupled to a lipophilic moiety), during the immunization or the inhalation phase. IFN-gamma inhibited human IgE production when given at the time of immunization, but not during inhalation. This effect was long-lasting as Dpt aerosol, given one month after immunization and IFN-gamma administration, failed to increase IgE levels. Unlike Dpt or p52-71, LP 52-71 failed to induce human IgE production at day 14 and 21 after its injection, but did inhibit the development of the IgE response after a secondary Dpt-challenge. Moreover, LP 52-71 administration 14 days after Dpt inhalation decreased IgE levels, in contrast to peptide 52-71, which increased IgE levels. Thus, taken together these results indicate that the development of the human IgE response in allergic SCID mice can be modulated by modified allergen and a Th1 cytokine.

  8. Quantitation of major allergens in dust samples from urban populations collected in different seasons in two climatic areas of the Basque region (Spain).

    Science.gov (United States)

    Echechipía, S; Ventas, P; Audícana, M; Urrutia, I; Gastaminza, G; Polo, F; Fernández de Corres, L

    1995-06-01

    We present the results of allergen content evaluation in 80 dust samples from 31 homes of atopic patients from two climatic areas (humid and subhumid), collected in two seasons of the year (autumn and winter). Monoclonal antibody-based immunoassays were used to quantify Der p 1, Der f 1, Der 2, Lep d 1, and Fel d 1. The results were compared according to climate, season, and the type of sensitization (Pyroglyphidae mites, storage mites, or grass pollens). We underline the predominance of Dermatophagoides pteronyssinus (89% of samples) over D. farinae (16% of samples) in our environment. Der p 1 rates were higher in the humid area (Mann-Whitney P < 0.001), especially in the autumn (Wilcoxon P < 0.05). Lep d 1 was detected in 23% of samples and Lep d 1 levels were higher in the homes of patients sensitized to storage mites (Mann-Whitney P < 0.05), whereas this allergen was not detected in the homes of pollen-allergic patients. Fel d 1 was detected in nine of the 31 homes (16% of samples) although there was a cat in only one home.

  9. The effect of marimastat, a metalloprotease inhibitor, on allergen-induced asthmatic hyper-reactivity

    International Nuclear Information System (INIS)

    Bruce, Colleen; Thomas, Paul S.

    2005-01-01

    This pilot study was designed to assess whether a synthetic matrix metalloproteinase (MMP) inhibitor has anti-inflammatory properties in mild asthma. Tumor necrosis factor alpha (TNFα) has been shown to be an important cytokine in the pathogenesis of allergic airway inflammatory responses, and its release can be inhibited by MMP inhibitors. Twelve atopic asthmatic subjects received the MMP inhibitor marimastat (5 mg) or placebo, twice daily for 3 weeks, separated by a 6-week washout period in a randomized, double-blind, cross-over manner. All subjects underwent an allergen inhalation provocation test to Dermatophagoides pteronyssinus before and after each study phase. Spirometry, exhaled NO (eNO) levels, differential sputum cell counts, an asthma symptom questionnaire, peak flow, and β 2 -agonist usage were measured. Nine subjects completed the study, and, when compared with placebo, marimastat reduced bronchial hyper-responsiveness to inhaled allergen in these subjects from an allergen PC 20 of 22.2 AU/ml (95%CI 11.7-32.6) to 17.0 AU/ml (95%CI 7.6-26.4, P = 0.02). The marimastat phase showed a nonsignificant fall in sputum inflammatory cells. Marimastat did not modify eNO, FEV 1 , asthma symptoms, or albuterol usage. In conclusion, airway responsiveness to allergen may be modified by a MMP inhibitor, perhaps via TNFα playing a role in airway inflammation and remodeling

  10. Ethnicity influences disease characteristics and symptom severity in allergic rhinitis patients in Malaysia.

    Science.gov (United States)

    Amini, Peyman; Abdullah, Maha; Seng, Lee Sing; Karunakaran, Thanusha; Hani, Norzhafarina; Bakar, Saraiza Abu; Latiff, Amir Hamzah Abdul; Fong, Seow Heng; Yeow, Yap Yoke

    2016-06-01

    The number of available reports regarding the influence of ethnicity on clinical features of allergic rhinitis (AR), especially disease severity in tropical climates, is limited. We aimed to compare clinical parameters and disease severity in AR patients of different ethnicities. Malay, Chinese, and Indian AR patients (n = 138) with confirmed sensitivity to Dermatophagoides pteronyssinus, Dematophagoides farinae, and Blomia tropicalis were tested for mite-specific immunoglobulin E (sIgE) levels. A detailed questionnaire was used to collect data on nasal symptom score (NSS), ocular symptom score (OSS), sum of symptoms score (SSS), quality of life score (QLS), symptomatic control score (SCS), and total sum of scores (TSS) and correlate the derived data with patients' demography, mite-polysensitivity, and sIgE levels. AR-related symptoms were most severe in Malays and least in Chinese (p Chinese. Duration of concurrent allergies was highest in Malays (p Chinese but correlated strongly with NSS, OSS, SSS, and TSS (r = 0408 to 0.898, p Chinese. © 2016 ARS-AAOA, LLC.

  11. Origins of asexuality in Bryobia mites (Acari: Tetranychidae)

    NARCIS (Netherlands)

    Ros, V.I.D.; Breeuwer, J.A.J.; Menken, S.B.J.

    2008-01-01

    Background Obligate asexual reproduction is rare in the animal kingdom. Generally, asexuals are considered evolutionary dead ends that are unable to radiate. The phytophagous mite genus Bryobia contains a large number of asexual species. In this study, we investigate the origin and evolution of

  12. Vectorial role of some dermanyssoid mites (Acari, Mesostigmata, Dermanyssoidea

    Directory of Open Access Journals (Sweden)

    Valiente Moro C.

    2005-06-01

    Full Text Available Among transmissible diseases, vectorial diseases represent a major problem for public health. In the group of acarina, while ticks are the most commonly implicated vectors, other arthropods and notably Dermanyssoidea are also involved in the transmission of pathogenic agents. Since the role of this superfamily is at present largely unknown, we have reviewed the vectorial role of these mites in the appearance, survival and propagation of pathogens. Various authors have shown that Dermanyssoidea are implicated in the transmission of both bacteria (Salmonella, Spirocheta, Rickettsia or Pasteurella and viruses (equine encephalitis viruses, West Nile virus, Fowl pox virus, the virus causing Newcastle disease and tick borne encephalitis viruses or hantaviruses. Finally, some authors have also shown their role in the transmission of some protozoa and filaria. As the vectorial character of such mites has been more clearly demonstrated (Dermanyssus gallinae, Ornithonyssus bacoti and Allodermanyssus sanguineus, it would be interesting to continue studies to better understand the role of this superfamily in the epidemiology of certain zoonoses.

  13. Six new species of ptyctimous mites (Acari: Oribatida) from Madagascar

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2016-01-01

    Roč. 56, č. 4 (2016), s. 485-496 ISSN 0044-586X Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Euphthiracaroidea * Madagascar * morphology * new species * ptyctimous mites * taxonomy Subject RIV: EG - Zoology Impact factor: 0.667, year: 2016

  14. Assessing hygienic behavior and attraction to Varroa mite (Acari ...

    African Journals Online (AJOL)

    user

    2011-02-07

    Feb 7, 2011 ... treatment and then the selected 5th instar larva were transferred to fundamental colonies with 10 to 12 ... Key words: Varroa mite, hygienic behavior (uncapping and .... into a container containing hot water and detergent.

  15. Biology of Dermacentor marginatus (Acari: Ixodidae under laboratory conditions

    Directory of Open Access Journals (Sweden)

    Mohammad Mehdi Darvishi

    2014-02-01

    Full Text Available Objective: To investigate and survey the biology of Dermacentor marginatus (D. marginatus under laboratory conditions. Methods: In this investigation, D. marginatus adult ticks were collected from sheep in Semnan province. Then various developmental stages of D. marginatus including larvae, nymphs and adult males and females under laboratory condition were bred and their biology was scrutinized. Results: The requisite time to complete the life cycle of D. marginatus under controlled laboratory conditions for larvae (26 °C, 75% relative humidity and nymph (26 °C, 95% relative humidity moulting, was on average 92 d (range 75-104 d, including preoviposition and egg incubation (22.5 d, larvae incubation (20.5 d, nymphal stage (28 d along with male maturation (21 d. The index of conversion efficiency and the index of reproduction efficiency in females were 0.397 and 8.300, respectively. Conclusions: Although in this investigation, there was no meaningful correlation between preoviposition period and the weight of female ticks which were laid successfully. The significant linear relationship was fully observed between the weight of engorged female of D. marginatus and the number of eggs laid. The mean of preoviposition period from 5.4 d in autumn to 34.2 d in spring increased. The minimum weight of ticks with laying capacity was 69 mg and lighter ticks (21 mg either did not lay or if they did their eggs did not hatch.

  16. Relative humidity and activity patterns of Ixodes scapularis (Acari: Ixodidae)

    Science.gov (United States)

    Berger, K.A.; Ginsberg, Howard S.; Gonzalez, L.; Mather, T.N.

    2014-01-01

    Laboratory studies have shown clear relationships between relative humidity (RH) and the activity and survival of Ixodes scapularis Say (blacklegged tick). However, field studies have produced conflicting results. We examined this relationship using weekly tick count totals and hourly RH observations at three field sites, stratified by latitude, within the state of Rhode Island. Records of nymphal tick abundance were compared with several RH-related variables (e.g., RH at time of sampling and mean weekly daytime RH). In total, 825 nymphs were sampled in 2009, a year of greater precipitation, with a weighted average leaf litter RH recorded at time of sampling of 85.22%. Alternatively, 649 nymphs were collected in 2010, a year of relatively low precipitation, and a weighted average RH recorded at time of sampling was 75.51%. Negative binomial regression analysis of tick count totals identified cumulative hours <82% RH threshold as a significant factor observed in both years (2009: P = 0.0037; 2010: P < 0.0001). Mean weekly daytime RH did not significantly predict tick activity in either year. However, mean weekly daytime RH recorded with 1-wk lag before sample date was a significant variable (P = 0.0016) in 2010. These results suggest a lag effect between moisture availability and patterns of tick activity and abundance. Differences in the relative importance of each RH variable between years may have been due to abnormally wet summer conditions in 2009.

  17. Parasites and pathogens of ticks ( Rhipicephalus species Acari ...

    African Journals Online (AJOL)

    The interaction of ticks with its environment as well as its natural hosts predisposes it to acquiring pathogens that could pose animal and human health risks. Identifying these pathogens could alert dog owners and others to reassess the predisposing factors and ensure control. The aim of the study was to identify the species ...

  18. The ticks (Acari: Ixodida: Argasidae, Ixodidae) of Bolivia.

    Science.gov (United States)

    Mastropaolo, Mariano; Beltrán-Saavedra, L Fabián; Guglielmone, Alberto A

    2014-03-01

    The tick species reported in Bolivia are reviewed here as (1) endemic or established: Ornithodoros echimys, O. guaporensis, O. hasei, O. kohlsi, O. mimon, O. peropteryx, O. rostratus, Otobius megnini, Amblyomma auricularium, A. cajennense, A. calcaratum, A. coelebs, A. dubitatum, A. humerale, A. incisum, A. longirostre, A. naponense, A. nodosum, A. oblongoguttatum, A. ovale, A. parvitarsum, A. parvum, A. pecarium, A. pseudoconcolor, A. rotundatum, A. scalpturatum, A. tigrinum, A. triste, Dermacentor nitens, Haemaphysalis juxtakochi, H. leporispalustris, I. boliviensis, I. cooleyi, I. luciae, Rhipicephalus microplus, R. sanguineus, and (2) erroneously reported: Ornithodoros puertoricensis, O. talaje, O. turicata, Amblyomma americanum, A. maculatum, A. multipunctum, Ixodes ricinus, I. scapularis, Rhipicephalus annulatus. Many of these records are lacking locality and/or host, and some of them need new findings for confirmation. Some of the species recorded may represent a threat for human and animal health, therefore would be of great value to make a countrywide survey of ticks in order to update the information presented in this work. Copyright © 2013 Elsevier GmbH. All rights reserved.

  19. Oribatid mites from the Vohimana Reserve, Madagascar (Acari: Oribatida, II

    Directory of Open Access Journals (Sweden)

    Mahunka, S.

    2009-11-01

    Full Text Available Further studies on the oribatid species collected from the Vohimana Reserve (Madagascar are presented. Altogether 13 species are listed, of them two represent new genera (Rugocepheus gen. nov. and Madabelba gen. nov., furthermore seven species are new to science. The other six species were earlier mentioned from different regions of the island, they are however little known.

  20. Contribution to the Uropodina mites of Peru (Acari: Mesostigmata).

    Science.gov (United States)

    Kontschán, Jenő; Friedrich, Stefan

    2017-02-27

    Soil dwelling Uropodina mites were collected from a primary lowland rainforest in Amazonian Peru. The species found belong to three different families. A new diagnosis and the type genus of Tetrasejaspidae fam. nov. are given, and the family is recorded from Peru for the first time on the basis of Tetrasejaspis sellnicki Hirschmann, 1973. Two rotundabaloghid mites were collected (Rotundabaloghia (Circobaloghia) magna Hirschmann, 1992 and Rotundabaloghia (Circobaloghia) iquitosensis Hirschmann, 1992), both already reported from Peru. A new species (Origmatrachys peruensis sp. nov.) from the family Trachyuropodidae was collected in large numbers from soil, and is described on the basis of females, males, nymphs and larvae. This is the first description of the protonymphs and larvae of Origmatrachys. The new species differs from the previously described ones in the basis of sculptural pattern of dorsal, ventral, sternal shields and the length of the setae in the central part of the dorsal shield. A new key to the known adults and deutonymphs of Origmatrachys is given.

  1. Heavy metal sensitivity and bioconcentration in oribatid mites (Acari, Oribatida)

    Energy Technology Data Exchange (ETDEWEB)

    Skubala, Piotr, E-mail: piotr.skubala@us.edu.pl [Department of Ecology, University of Silesia, Bankowa 9, 40-007 Katowice (Poland); Zaleski, Tomasz [Department of Soil Science and Soil Protection, Agricultural University in Krakow, Mickiewicza 21, 31-120 Cracow (Poland)

    2012-01-01

    In this study we aimed to identify different reactions of oribatid species to heavy metal pollution and to measure concentrations of cadmium, zinc and copper in oribatid species sampled along a gradient. Oribatid mites were sampled seasonally during two years in five meadows located at different distances from the zinc smelter in the Olkusz District, southern Poland. Oribatids were shown to withstand critical metal concentration and established comparatively abundant and diverse communities. The highest abundance and species richness of oribatids were recorded in soils with moderate concentrations of heavy metals. Four different responses of oribatid species to heavy metal pollution were recognized. Heavy metals (Zn, Pb, Cd, Ni) and various physical (bulk density, field capacity, total porosity) and chemical (K{sub av}, P{sub av}, N, C, pH) factors were recognized as the structuring forces that influence the distribution of oribatid species. Analysis by atomic absorption spectrophotometry revealed large differences in metal body burdens among species. None of the species can be categorized as accumulators or non-accumulators of the heavy metals - the pattern depends on the metal. The process of bioconcentration of the toxic metal (regulated) and essential elements (accumulated) was generally different in the five oribatid species studied. - Highlights: Black-Right-Pointing-Pointer Responses of oribatid mites to metal contamination along a gradient in meadow soils were studied. Black-Right-Pointing-Pointer Small concentrations of heavy metals positively influenced the abundance of oribatid mites. Black-Right-Pointing-Pointer Four different responses of oribatid species to heavy metal pollution were recognised. Black-Right-Pointing-Pointer Bioaccumulation of the toxic metal and essential elements proceeded differently in oribatid species. Black-Right-Pointing-Pointer Five studied oribatid species were deconcentrators of cadmium.

  2. Widespread Rickettsia spp. Infections in Ticks (Acari: Ixodoidea) in Taiwan.

    Science.gov (United States)

    Kuo, Chi-Chien; Shu, Pei-Yun; Mu, Jung-Jung; Lee, Pei-Lung; Wu, Yin-Wen; Chung, Chien-Kung; Wang, Hsi-Chieh

    2015-09-01

    Ticks are second to mosquitoes as the most important disease vectors, and recent decades have witnessed the emergence of many novel tick-borne rickettsial diseases, but systematic surveys of ticks and tick-borne rickettsioses are generally lacking in Asia. We collected and identified ticks from small mammal hosts between 2006 and 2010 in different parts of Taiwan. Rickettsia spp. infections in ticks were identified by targeting ompB and gltA genes with nested polymerase chain reaction. In total, 2,732 ticks were collected from 1,356 small mammals. Rhipicephalus haemaphysaloides Supino (51.8% of total ticks), Haemaphysalis bandicota Hoogstraal & Kohls (28.0%), and Ixodes granulatus Supino (20.0%) were the most common tick species, and Rattus losea Swinhoe (44.7% of total ticks) and Bandicota indica Bechstein (39.9%) were the primary hosts. The average Rickettsia infective rate in 329 assayed ticks was 31.9% and eight Rickettsia spp. or closely related species were identified. This study shows that rickettsiae-infected ticks are widespread in Taiwan, with a high diversity of Rickettsia spp. circulating in the ticks. Because notifiable rickettsial diseases in Taiwan only include mite-borne scrub typhus and flea-borne murine typhus, more studies are warranted for a better understanding of the real extent of human risks to rickettsioses in Taiwan. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  3. Ixodid Ticks (Acari, Ixodidae in Urban Landscapes. A review

    Directory of Open Access Journals (Sweden)

    Akimov I. А.

    2016-04-01

    Full Text Available This study presents the results of content analysis of published works on ixodid ticks in urban conditions in order to determine the species diversity, the vectors of research interests at various stages. Information about ticks in the cities up to the 1980s is incidental, to the point of exclusive, after this point there is targeted research in urban landscapes. There are 106 or 15 % of hard ticks of the world fauna registered in the urban territory, 26 species or 3.7 % being the most abundant. Of the urban hard tick species, 23 (88.5 % can attack humans, and 12 species are the most adapted to the urban landscape: Ixodes ricinus, I. persulcatus, Dermacentor reticulatus, D. marginatus, I. pavlovskyi, I. scapularis (dammini, Amblyomma cajennense, Haemaphysalis longicornis, I. hexagonus, Hyalomma marginatum, Am. americanum, and Rhipicephalus sanguineus. It was determined that the most likely causes of the growing number of publications on ixodids urban landscapes are: global accelerating urbanization, the development of recreational areas, the development of green tourism, the growth of the prestige of outdoor recreation, the creation of new, especially of the landscape parks and a tendency to preserve the native landscape in the cities, a significant increase in the density of populations of common species of hard ticks adapted to living in urban environment. The vectors of further work in urban landscapes will be directed to exact planning of monitoring studies of ixodids and associated tick-borne infections.

  4. A Proteomic Analysis of Sarcoptes scabiei (Acari: Sarcoptidae)

    Science.gov (United States)

    Morgan, Marjorie S.; Arlian, Larry G.; Rider, S. Dean; Grunwald, William C.; Cool, David R.

    2016-01-01

    The pruritic skin disease scabies is caused by the burrowing of the itch mite Sarcoptes scabiei (De Geer). It is difficult to diagnose this disease because its symptoms often resemble those of other skin diseases. No reliable blood or molecular diagnostic test is available. The aim of this project was to begin to characterize the scabies proteome to identify scabies mite proteins, including those that may be useful in the development of a diagnostic test or vaccine. Various scabies mite extracts were separated by two-dimensional electrophoresis, and 844 Coomassie Blue-stained protein spots were excised, subjected to trypsin digestion, and analyzed by Matrix Assisted Laser Desorption/Ionization Time-Of-Flight/Time-Of-Flight (MALDI-TOF/TOF) mass spectrometry (MS). Tryptic fragment sequences determined by MS were searched against the recently completed S. scabiei annotated genome, leading to the identification of >150 proteins. Only 10 proteins hit to previously identified scabies proteins including actin, tropomyosin, and several ABC transporters. Thirteen proteins had homology to dust mite allergens (members of groups 8, 10, 13, 17, 20, 25, and 28). Most other sequences showed some homology to proteins in other mites and ticks including homologs of calmodulin, calreticulin, lipocalin, and glutathione-S-transferase. These data will now allow the identification of the proteins to which scabies patients produce antibodies, including those that may be good candidates for inclusion in a diagnostic test and vaccine. PMID:26792847

  5. The ticks (Acari: Ixodida: Argasidae, Ixodidae) of Paraguay

    Science.gov (United States)

    2007-01-01

    20u209S, 60u209W Parque Defensores del Chaco 20u149S, 60u129W ALTO PARANÁ Ciudad del Este 25u329S, 54u379W BOQUERÓN Colonia Fernheim 22u149S, 59u509W...female, host unknown, Parque Defensores del Chaco, 5 December 1981, coll. J. A. Kochalka), RML 119014 (two males and one female, ex Catagonus wagneri...Moro, 22 June 2002, coll. M. Cunningham), RML 123661 (one female, ex Pa. onca, Parque Defensores del Chaco, 16 July 2003, coll. M. Cunningham), RML

  6. Assessing hygienic behavior and attraction to Varroa mite (Acari ...

    African Journals Online (AJOL)

    Three days later, the number of female Varroa in the capped cells were counted as attraction criteria for each treatment separately. ... The negative correlation between 2 major resistance traits (hygienic behavior and attraction traits) in the breeding stock deems that selection for resistance against Varroa mite and improved ...

  7. Resistance of the predacious mite, euseius kenyae (acari ...

    African Journals Online (AJOL)

    This study was carried out to assess whether the predacious phytoseiid mite, Euseius kenyae (Swirski and Ragusa), commonly found in major coffee growing regions in Kenya has developed resistance to Chlorpyrifos. Mite populations were collected from coffee farms harbouring E. kenyae and where Chlorpyrifos or other ...

  8. Listado de oribátidos (Acari, Oribatida de Túnez

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    Subías, L. S.

    2015-12-01

    Full Text Available The oribatid mites of several Tunisian soil samples were studied. 113 species were obtained, 95 of which are recorded for the first time from Tunisia. A systematic check-list with the 136 species known up to now is included, and their geographical distribution is given. Most of them, 77 (56%, have a typical Mediterranean distribution and 16 are recorded for the first time from North Africa.Se han estudiado los ácaros oribátidos de una serie de muestras de suelo de Túnez y se han identificado 113 especies, 95 de las cuales se citan por primera vez en Túnez. Se ha elaborado un listado sistemático en el que se incluyen las 136 especies conocidas hasta el momento y su distribución geográfica. La mayoría, 77 (56 %, presentan características típicamente mediterráneas, y 16 se citan por primera vez en el norte de África.

  9. A supplementary description of Brevipalpus californicus (Acari: Trombidiformes: Tenuipalpidae

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    Mohammad Raissi Ardali

    2015-10-01

    Full Text Available The false spider mite Brevipalpus californicus from the family Tenuipalpidae was collected from Caucasian alnus, White willow, Persian raspberry and a wild Chrysanthemum bush in Mazandaran province. This species is reported as a new record to the false spider mites-fauna of Iran here. Reviewing literatures revealed that it was briefly described in the original paper without any measures. So, a completed description is presented based on the Iranian specimens and different body segments are drawn for B. californicus. In addition, the above plants are new host records for B. californicus.

  10. Can Euseius alatus DeLeon (Acari: Phytoseiidae) prey on Aceria guerreronis Keifer (Acari: Eriophyidae) in coconut palm?; Pode Euseius alatus DeLeon (Acari: Phytoseiidae) predar Aceria guerreronis Keifer (Acari: Eriophyidae) em coqueiro?

    Energy Technology Data Exchange (ETDEWEB)

    Melo, Jose W. da S.; Domingos, Cleiton A.; Gondim Junior, Manoel G.C. [Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE (Brazil). Dept. de Agronomia. Area de Fitossanidade]. E-mail: mguedes@depa.ufrpe.br; Moraes, Gilberto J. de [Universidade de Sao Paulo (USP), Piracicaba, SP (Brazil). Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Dept. de Entomologia, Fitopatologia e Zoologia Agricola]. E-mail: gjmoraes@carpa.ciagri.usp.br

    2009-01-15

    Mites of the genus Euseius are generally considered specialist as pollen feeders. Euseius alatus DeLeon is one of the six species of phytoseiid mites most commonly found on coconut plants in northeast Brazil associated with Aceria guerreronis Keifer. Although the morphology of E. alatus does not favor the exploitation of the meristematic area of the fruit inhabited by A. guerreronis, the predator may have some role in the control of this eriophyid during the dispersion process. The objective of this work was to evaluate the development and reproduction of E. alatus on the following diets: A. guerreronis, Ricinus communis pollen (Euphorbiaceae), and Tetranychus urticae Koch (Tetranychidae) + R. communis pollen + honey solution 10%. Euseius alatus developed slightly faster and had slightly higher oviposition rate when feeding on the diet composed of T. urticae + pollen + honey. However, life table parameters were very similar on all diets, suggesting that E. alatus may contribute in reducing the population of A. guerreronis in the field. (author)

  11. Plant species modifies the functional response of Phytoseiulus persimilis (Acari: Phytoseiidae) to Tetranychus urticae (Acari: Tetranychidae): implications for biological control.

    Science.gov (United States)

    Skirvin, D J; Fenlon, J S

    2001-02-01

    The functional response of the predatory mite Phytoseiulus persimilis Athias-Henriot to eggs of its prey, the spider mite Tetranychus urticae Koch was examined on three plant species. Experiments were done to determine whether differences in the functional response on the three plant species were due to the morphological features of the crop directly on the predator or through an effect of the plant species on the prey. The results show that crop morphology is the only factor influencing the predatory ability of P. persimilis on the three plant species. Fewer eggs were eaten on Ceanothus thyrsiflorus var. 'Autumnal Blue', the plant species with hairy leaves, and greater numbers of prey consumed on Choisya ternata, a species with smooth leaves. However, similarly few eggs were eaten on the smooth, but waxy leaved Euonymus japonicus as on Ceanothus thyrsiflorus, demonstrating that morphological characters of leaves other than the possession of hairs and trichomes may affect the rates of predation. The implications of these results for the tritrophic interactions between plant, predator and prey, and the development of suitable biological control strategies are discussed.

  12. Alternating temperatures affect life table parameters of Phytoseiulus persimilis, Neoseiulus californicus (Acari: Phytoseiidae) and their prey Tetranychus urticae (Acari: Tetranychidae).

    Science.gov (United States)

    Vangansbeke, Dominiek; De Schrijver, Lien; Spranghers, Thomas; Audenaert, Joachim; Verhoeven, Ruth; Nguyen, Duc Tung; Gobin, Bruno; Tirry, Luc; De Clercq, Patrick

    2013-11-01

    Increasing energy costs force glasshouse growers to switch to energy saving strategies. In the temperature integration approach, considerable daily temperature variations are allowed, which not only have an important influence on plant growth but also on the development rate of arthropods in the crop. Therefore, we examined the influence of two constant temperature regimes (15 °C/15 °C and 20 °C/20 °C) and one alternating temperature regime (20 °C/5 °C, with an average of 15 °C) on life table parameters of Phytoseiulus persimilis and Neoseiulus californicus and their target pest, the two-spotted spider mite Tetranychus urticae at a 16:8 (L:D) h photoperiod and 65 ± 5 % RH. For females of both predatory mites the alternating temperature regime resulted in a 25-30 % shorter developmental time as compared to the corresponding mean constant temperature regime of 15 °C/15 °C. The immature development of female spider mites was prolonged for 7 days at 15 °C/15 °C as compared to 20 °C/5 °C. With a daytime temperature of 20 °C, no differences in lifetime fecundity were observed between a nighttime temperature of 20 and 5 °C for P. persimilis and T. urticae. The two latter species did show a higher lifetime fecundity at 20 °C/5 °C than at 15 °C/15 °C, and their daily fecundity at the alternating regime was about 30 % higher than at the corresponding mean constant temperature. P. persimilis and T. urticae showed no differences in sex ratio between the three temperature regimes, whereas the proportion of N. californicus females at 15 °C/15 °C (54.2 %) was significantly lower than that at 20 °C/5 °C (69.4 %) and 20 °C/20 °C (67.2 %). Intrinsic rates of increase were higher at the alternating temperature than at the corresponding mean constant temperature for both pest and predators. Our results indicate that thermal responses of the studied phytoseiid predators to alternating temperature regimes used in energy saving strategies in glasshouse crops may have consequences for their efficacy in biological control programs.

  13. Olfactory response of Phytoseiulus persimilis (Acari: Phytoseiidae) to untreated and Beauveria bassiana-treated Tetranychus urticae (Acari: Tetranychidae).

    Science.gov (United States)

    Seiedy, Marjan; Saboori, Alireza; Zahedi-Golpayegani, Azadeh

    2013-06-01

    Determination of attraction and avoidance behavior of predators is important in concomitant use of multiple natural enemies to control a pest. The olfactory response of the predatory mite Phytoseiulus persimilis was studied to odors related to Tetranychus urticae adults infected by Beauveria bassiana DEBI008 in 0, 24, 48 and 72 h intervals, both in absence and in presence of plants. In plant-present experiments, P. persimilis attraction was neither towards adults of T. urticae infected by 0.02 % Tween 80 (as control), nor to the ones infected by B. bassiana for 0 or 24 h, whereas significant attraction towards the control was observed when tested against T. urticae infected by B. bassiana for 48 or 72 h. In absence of plants, P. persimilis displayed significant avoidance of T. urticae infected by B. bassiana for 48 or 72 h, when their alternative option was 0.02 % Tween 80-infected T. urticae adults. These results indicate that P. persimilis can recognize the presence of B. bassiana and that the predator avoids the fungus. This suggests that the two natural enemy species can be used together in biological control programmes.

  14. Effects of azadirachtin on Tetranychus urticae (Acari: Tetranychidae) and its compatibility with predatory mites (Acari: Phytoseiidae) on strawberry.

    Science.gov (United States)

    Bernardi, Daniel; Botton, Marcos; da Cunha, Uemerson Silva; Bernardi, Oderlei; Malausa, Thibaut; Garcia, Mauro Silveira; Nava, Dori Edson

    2013-01-01

    The spider mite, Tetranychus urticae, is the major strawberry pest in Brazil. The main strategies for its control comprise synthetic acaricides and predatory mites. The recent register of a commercial formula of azadirachtin (Azamax(®) 12 g L(-1) ) can be viable for control of T. urticae. In this work, the effects of azadirachtin on T. urticae and its compatibility with predatory mites Neoseiulus californicus and Phytoseiulus macropilis in the strawberry crop were evaluated. Azadirachtin was efficient against T. urticae, with a mortality rate similar to that of abamectin. In addition, the azadirachtin showed lower biological persistence (7 days) than abamectin (21 days). Azadirachtin did not cause significant mortality of adult predatory mites (N. californicus and P. macropilis), but it did reduce fecundity by 50%. However, egg viability of the azadirachtin treatments was similar to that of the control (>80% viability). The use of azadirachtin and predatory mites is a valuable tool for controlling T. urticae in strawberry crop. Azadirachtin provided effective control of T. urticae and is compatible with the predatory mites N. californicus and P. macropilis. It is an excellent tool to be incorporated into integrated pest management for strawberry crop in Brazil. Copyright © 2012 Society of Chemical Industry.

  15. Can Euseius alatus DeLeon (Acari: Phytoseiidae) prey on Aceria guerreronis Keifer (Acari: Eriophyidae) in coconut palm?

    International Nuclear Information System (INIS)

    Melo, Jose W. da S.; Domingos, Cleiton A.; Gondim Junior, Manoel G.C.; Moraes, Gilberto J. de

    2009-01-01

    Mites of the genus Euseius are generally considered specialist as pollen feeders. Euseius alatus DeLeon is one of the six species of phytoseiid mites most commonly found on coconut plants in northeast Brazil associated with Aceria guerreronis Keifer. Although the morphology of E. alatus does not favor the exploitation of the meristematic area of the fruit inhabited by A. guerreronis, the predator may have some role in the control of this eriophyid during the dispersion process. The objective of this work was to evaluate the development and reproduction of E. alatus on the following diets: A. guerreronis, Ricinus communis pollen (Euphorbiaceae), and Tetranychus urticae Koch (Tetranychidae) + R. communis pollen + honey solution 10%. Euseius alatus developed slightly faster and had slightly higher oviposition rate when feeding on the diet composed of T. urticae + pollen + honey. However, life table parameters were very similar on all diets, suggesting that E. alatus may contribute in reducing the population of A. guerreronis in the field. (author)

  16. Ocorrência de Brevipalpus phoenicis (Geijskes (Acari, Tenuipalpidae, Tetranychus urticae (Koch (Acari, Tetranychidae e Polyphagotarsonemus latus (Banks (Acari, Tarsonemidae sobre folhas de Ipomoea cairica (Linnaeus Sweet (Solanales, Convolvulaceae Occurrence of Brevipalpus phoenicis (Geijskes (Acari, Tenuipalpidae, Tetranychus urticae (Koch (Acari, Tetranychidae and Polyphagotarsonemus latus (Banks (Acari, Tarsonemidae on leaves of I. cairica (Linnaeus Sweet (Solanales, Convolvulaceae

    Directory of Open Access Journals (Sweden)

    Ozana M. de A. Maia

    2006-09-01

    Full Text Available A ocorrência de três espécies acarinas fitófagas é relatada pela primeira vez sobre folhas de Ipomoea cairica. As espécies Brevipalpus phoenicis (Geijskes, Tetranychus urticae (Koch e Polyphagotarsonemus latus (Banks, foram coletadas sobre folhas de I. cairica nas imediações da Universidade Federal do Paraná, Curitiba, Paraná, Brasil, em 20 de janeiro de 2005.The first occurrence of three phytophagus mites on Ipomoea cairica, is reported. The species Brevipalpus phoenicis (Geijskes, Tetranychus urticae (Koch and Polyphagotarsonemus latus (Banks were caught on leaves of I. cairica, around Universidade Federal do Paraná, Curitiba, Paraná, Brazil, in January 20th, 2005.

  17. Sick building syndrome (SBS) among office workers in a Malaysian university--Associations with atopy, fractional exhaled nitric oxide (FeNO) and the office environment.

    Science.gov (United States)

    Lim, Fang-Lee; Hashim, Zailina; Md Said, Salmiah; Than, Leslie Thian-Lung; Hashim, Jamal Hisham; Norbäck, Dan

    2015-12-01

    There are few studies on sick building syndrome (SBS) including clinical measurements for atopy and fractional exhaled nitric oxide (FeNO). Our aim was to study associations between SBS symptoms, selected personal factors, office characteristics and indoor office exposures among office workers from a university in Malaysia. Health data were collected by a questionnaire (n=695), skin prick test (SPT) (n=463) and FeNO test (n=460). Office settled dust was vacuumed and analyzed for endotoxin, (1,3)-β-glucan and house dust mites (HDM) allergens group 1 namely Dermatophagoides pteronyssinus (Der p 1) and Dermatophagoides farinae (Der f 1). Office indoor temperature, relative air humidity (RH), carbon monoxide (CO) and carbon dioxide (CO2) were measured by a direct reading instrument. Associations were studied by two-levels multiple logistic regression with mutual adjustment and stratified analysis. The prevalence of weekly dermal, mucosal and general symptoms was 11.9%, 16.0% and 23.0% respectively. A combination of SPT positivity (allergy to HDM or cat) and high FeNO level (≥25 ppb) was associated with dermal (p=0.002), mucosal (p<0.001) and general symptoms (p=0.05). Der f1 level in dust was associated with dermal (p<0.001), mucosal (p<0.001) and general (p=0.02) symptoms. Among those with allergy to D. farinae, associations were found between Der f 1 levels in dust and dermal (p=0.003), mucosal (p=0.001) and general symptoms (p=0.007). Office-related symptoms were associated with Der f 1 levels in dust (p=0.02), low relative air humidity (p=0.04) and high office temperature (p=0.05). In conclusion, a combination of allergy to cat or HDM and high FeNO is a risk factor for SBS symptoms. Der f 1 allergen in dust can be a risk factor for SBS in the office environment, particularly among those sensitized to Der f 1 allergen. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Prevalence of immunoglobulin E-mediated food allergy in 6-9-year-old urban schoolchildren in the eastern Black Sea region of Turkey.

    Science.gov (United States)

    Orhan, F; Karakas, T; Cakir, M; Aksoy, A; Baki, A; Gedik, Y

    2009-07-01

    The prevalence of adverse reactions to food in childhood in Turkey is not known. We aimed to investigate the prevalence and characteristics of IgE-mediated food allergies (FAs) in 6-9-year-old urban schoolchildren. This cross-sectional study recruited 3500 of the randomly selected 6-9-year-old urban schoolchildren from the eastern Black Sea region of Turkey during 2006. Following a self-administered questionnaire completed by the parents and the child, consenting children were invited for skin prick tests (SPTs) and oral food challenges. Children with suspected IgE-mediated FA were skin prick tested with a predefined panel of food allergens (milk, hen's egg, soy, wheat, peanut, fish, and hazelnut), aeroallergens (Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat, dog, Alternaria, grass pollen mix, weed pollen mix, and tree pollen mix), and food allergens reported in the questionnaire. All children with a positive SPT to any food were invited for a double-blind, placebo-controlled food challenge (DBPCFC). The prevalence of IgE-mediated FA was established using DBPCFCs. The response rate to the questionnaire was 78.2% (2739/3500). The estimated prevalence of parental-reported IgE-mediated FA was 5.7% (156/2739) [95% confidence interval (CI), 4.83-6.57%]. The rate of sensitization to the food allergens was 33.1% (48/145) in the parental-reported group. The confirmed prevalence of IgE-mediated FA by means of DBPCFC in 6-9-year-old urban schoolchildren living in the eastern Black Sea region of Turkey was 0.80% (22/2739) (95% CI, 0.47-1.13%). The most common allergenic foods were beef (31.8%), cow's milk (18.1%), cocoa (18.1%), hen's egg (13.6%), and kiwi (13.6%). The rate of reported IgE-mediated FA was significantly higher than clinically confirmed FA by means of DBPCFC (odds ratio, 7.46; 95% CI, 4.67-12.01; Pfoods was different and somewhat unique to the eastern Black Sea region of Turkey when compared with western countries.

  19. Avaliação da hiperresponsividade brônquica à solução salina hipertônica em crianças e adolescentes Bronchial hyperresponsiveness to hypertonic saline challenge in children and adolescents

    Directory of Open Access Journals (Sweden)

    Paulo Kussek

    2006-06-01

    Full Text Available OBJETIVO: Avaliar a hiperresponsividade brônquica à solução salina hipertônica a 4,5% como método alternativo a outros agentes broncoconstritores e sua relação com a sensibilização alérgica do paciente. MÉTODOS: Estudo transversal, experimental, com 85 indivíduos assim distribuídos: 45 no grupo de asmáticos e 17 no grupo controle não asmáticos e não alérgicos, que completaram o teste. Para nebulizar a solução salina hipertônica foi utilizado um nebulizador ultra-sônico de grande volume, sucessivamente durante 0,5, 1, 2, 4 e 8 minutos até haver queda > 15% em relação ao volume expiratório forçado no primeiro segundo basal. A dosagem de imunoglobulina E específica ao Dermatophagoides pteronyssinus por ImmunoCap foi considerada positiva quando > 0,35 kU/L. RESULTADOS: No grupo de asmáticos, 36 apresentaram queda média do volume expiratório forçado no primeiro segundo de 27,4% após nebulização de solução salina hipertônica. Nenhum do grupo controle (imunoglobulina E OBJECTIVE: To assess airway hyperresponsiveness to 4.5% hypertonic saline solution in comparison to that obtained through challenge with other bronchoconstriction agents and in relation to patient allergic sensitization. METHODS: A cross-sectional, experimental study was conducted, initially involving 85 subjects. After exclusions, the final sample consisted of 62 patients, divided into two groups: a study group of those with asthma (n = 45 and a control group of those with no asthma or allergies (n = 17. Hypertonic saline was nebulized using an ultrasonic nebulizer and administered successively for 0.5, 1, 2, 4 and 8 minutes until a drop in forced expiratory volume in one second of = 15% was achieved in relation to the baseline value. The level of specific immunoglobulin E to Dermatophagoides pteronyssinus level was determined by ImmunoCAP assay and was considered positive when > 0.35 kU/L. RESULTS: In the 36 asthma group subjects presenting a

  20. Conjugation of nitrated acetaminophen to Der p1 amplifies peripheral blood monocyte response to Der p1.

    Directory of Open Access Journals (Sweden)

    Ryan G Thomas

    Full Text Available An association of acetaminophen use and asthma was observed in the International Study of Asthma and Allergies in Childhood study. However there are no clear mechanisms to explain an association between acetaminophen use and immunologic pathology. In acidic conditions like those in the stomach and inflamed airway, tyrosine residues are nitrated by nitrous and peroxynitrous acids. The resulting nitrotyrosine is structurally similar to 2,4-dinitrophenol and 2,4-dinitrochlorobenzene, known haptens that enhance immune responses by covalently binding proteins. Nitrated acetaminophen shares similar molecular structure.We hypothesized the acetaminophen phenol ring undergoes nitration under acidic conditions, producing 3-nitro-acetaminophen which augments allergic responses by acting as a hapten for environmental allergens.3-nitro-acetaminophen was formed from acetaminophen in the presence of acidified nitrite, purified by high performance liquid chromatography, and assayed by gas-chromatography mass spectrometry. Purified 3-nitro-acetaminophen was reacted with Dermatophagoides pteronyssinus (Der p1 and analyzed by mass spectrometry to identify the modification site. Human peripheral blood mononuclear cells proliferation response was measured in response to 3-nitro-acetaminophen and to 3-nitro-acetaminophen-modified Der p1.Acetaminophen was modified by nitrous acid forming 3-nitro-acetaminophen over a range of different acidic conditions consistent with airway inflammation and stomach acidity. The Der p1 protein-hapten adduct creation was confirmed by liquid chromatography-mass spectrometry proteomics modifying cysteine 132. Peripheral blood mononuclear cells exposed to 3-nitro-acetaminophen-modified Der p1 had increased proliferation and cytokine production compared to acetaminophen and Der p1 alone (n = 7; p < 0.05.These data suggests 3-nitro-acetaminophen formation and reaction with Der p1 provides a mechanism by which stomach acid or infection

  1. HOUSE DUST MITE CONTAMINATION IN HOTELS AND INNS IN BANDAR ABBAS, SOUTH OF IRAN

    Directory of Open Access Journals (Sweden)

    M. Soleimani, J. Rafinejad

    2008-07-01

    Full Text Available House dust mites have been shown to be strongly associated with allergic respiratory diseases such as, bronchial asthma, rhinitis and atopic dermatitis in the world. The climatic conditions of Bandar-Abbas, which is located in a coastal area and has a humid subtropical climate, provide a suitable place to proliferate mites. The aim of this study was to determine the contamination rate and analyze the house dust mite fauna in hotels and inns in Bandar-Abbas that had not been investigated previously. In this study 6 hotels and 6 inns were selected randomly in six areas of Bandar-Abbas. Two dust samples were collected from each place with a vacuum cleaner. One square meter of carpets and mattresses were vacuumed for a period of 1 min. Then the samples were cleared in lactic acid and then mites were mounted in Hoyer's medium for study and identification. A total of 2644 mites were collected and identified. The major mite family was Pyroglyphidae (98%. Dermatophagoides pteronyssinus was the most frequent and most numerous species recorded, occurring in 91% of samples examined and forming 88% of the Pyroglyphidae and 86% of the total mite populations. The family Cheyletidae was less commonly found with Cheyletus malaccensis (2%. Most of the mites were isolated from the carpets (57.5%, and a smaller number from mattresses (42.5%. Mites were present in 96% of the dust samples. Results revealed that all inns and 83% of hotels were contaminated by more than one species of mite and 34% of them had a population of more than 100 mites /g dust. This rate of contamination can be a major risk factor in asthma and other respiratory allergic diseases

  2. Concentrations of plasminogen activator inhibitor-1 (PAI-1 and urokinase plasminogen activator (uPA in induced sputum of asthma patients after allergen challenge.

    Directory of Open Access Journals (Sweden)

    Marcin Moniuszko

    2011-04-01

    Full Text Available Urokinase plasminogen activator (uPA and its inhibitor (PAI-1 are involved in tiisue remodeling and repair processes associated with acute and chronic inflammation. The aim of the study was to evaluate the effect of allergen challenge on concentration of uPA and PAI-1 in induced sputum of house dust mite allergic asthmatics (HDM-AAs. Thirty HDM-AAs and ten healthy persons (HCswere recruited for the study. In 24 HDM-AAs bronchial challenge with Dermatophagoides pteronyssinus (Dp and in 6 HDM-AAs sham challenege with saline were performed. In HDM-AAs sputum was induced 24 hours before (T0 and 24 hours (T24 after the challenge. Concentration of uPA and PAI-1 in induced sputum were determined using immunoenzymatic assays. At T0 in HDM-AAs mean sputum uPA (151 Âą 96 pg/ml and PAI-1 (4341 Âą 1262 pg/ml concentrations were higher than in HC (18.8 Âą 6.7 pg/ml; p=0.0002 and 596 Âą 180 pg/ml; p<0.0001; for uPA and PAI-1 respectively. After allergen challenge further increase in sputum uPA (187 Âą 144 pg/ml; p=0.03 and PAI-1 (6252 Âą 2323 pg/ml; p<0.0001 concentrations were observed. Moreover, in Dp challenged, but not in saline challenged HDM-AAs the mean uPA/PAI-1 ratio decreased significantly at T24. No significant increase in the studied parameters were found in sham challenged patients. In HDM-AAs allergen exposure leads to activation of the plasmin system in the airways. Greater increase of the PAI-1 concentration than uPA concentration after allergen challenge may promote airway remodeling and play an important role in the development of bronchial hyperreactivity.

  3. Innate immune response of alveolar macrophage to house dust mite allergen is mediated through TLR2/-4 co-activation.

    Directory of Open Access Journals (Sweden)

    Chia-Fang Liu

    Full Text Available House dust mite, Dermatophagoides pteronyssinus (Der p, is one of the major allergens responsible for allergic asthma. However, the putative receptors involved in the signalization of Der p to the innate immune cells are still poorly defined as well as the impact of their activation on the outcome of the allergen-induced cell response. We previously reported that the HDM activation of mouse alveolar macrophages (AM involves the TLR4/CD14 cell surface receptor complex. Here using a TLR ligand screening essay, we demonstrate that HDM protein extract engages the TLR2, in addition to the TLR4, in engineered TLR-transfected HEK cells but also in the MH-S mouse alveolar macrophage cell line model. Moreover we found that the concomitant recruitment of the MH-S cell's TLR2 and TLR4 receptors by the HDM extract activates the MyD88-dependent signaling pathway and leads to the secretion of the NF-κB regulated pro-inflammatory factors NO and TNF-α. However unlike with the canonical TLR4 ligand (i.e. the bacterial LPS mobilization of TLR4 by the HDM extract induces a reduced production of the IL-12 pro-inflammatory cytokine and fails to trigger the expression of the T-bet transcription factor. Finally we demonstrated that HDM extract down-regulates LPS induced IL-12 and T-bet expression through a TLR2 dependent mechanism. Therefore, we propose that the simultaneous engagement of the TLR2 and TLR4 receptors by the HDM extract results in a cross regulated original activation pattern of the AM which may contribute to the Th2 polarization of the allergen-induced immune response. The deciphering of these cross-regulation networks is of prime importance to open the way for original therapeutic strategies taking advantage of these receptors and their associated signaling pathways to treat allergic asthma.

  4. Clinical and immunological features of patients with atopy and concomitant HTLV-1 infection

    Directory of Open Access Journals (Sweden)

    F.P. Gaspar-Sobrinho

    2010-12-01

    Full Text Available Human T-cell lymphotropic virus type 1 (HTLV-1 induces an exacerbated type 1 immune response characterized by high spontaneous IFN-γ and TNF-α production. Allergic rhinitis and asthma are associated with the type 2 immune response, with elevated secretion of IL-4 and IL-5. The aim of this study was to characterize the immune response in atopic HTLV-1 carriers. The cytokine profile of atopic HTLV-1 carriers (N = 10; all females was compared with that of non-atopic HTLV-1 carriers (N = 14; 9 females and 5 males. Mean patient age of atopic and non-atopic groups was 45 ± 8 and 38 ± 11 years, respectively. All atopic HTLV-1 carriers had rhinitis with or without asthma and a skin prick test positive for Dermatophagoides pteronyssinus antigen 1 (Derp-1. There was no difference in cytokine levels between the two groups in unstimulated peripheral blood mononuclear cell cultures. In cultures stimulated with Derp-1, IFN-γ levels tended to be higher (P = 0.06 and IL-5 levels were higher (P = 0.02 in atopic HTLV-1 patients than in non-atopic subjects. In contrast, IL-10 was lower (P = 0.004 in atopic than in non-atopic HTLV-1-infected subjects. This study shows that HTLV-1 infection with an exaggerated type 1 immune response does not prevent atopy. In this case, the exacerbated type 1 and type 2 immune responses were due to a lack of IL-10 production, a cytokine that plays an important role in down-modulating type 1 and type 2 immune responses and in preventing the development of chronic inflammatory diseases.

  5. Peripheral blood CD161+ T cells from asthmatic patients are activated during asthma attack and predominantly produce IFN-gamma.

    Science.gov (United States)

    González-Hernández, Y; Pedraza-Sánchez, S; Blandón-Vijil, V; del Río-Navarro, B E; Vaughan, G; Moreno-Lafont, M; Escobar-Gutiérrez, A

    2007-04-01

    In humans, T cells expressing the CD161 molecule NKR-P1A constitute around 20% of the circulating CD3(+) cells and are potentially immunoregulatory in several diseases. Their role in asthma is not well known, but they could participate in asthma attacks. To determinate whether activation of CD161(+) T cells and their cytokine production correlate with clinical status of asthma, we analysed blood samples from asthma attack patients (AAP) and stable asthma patients (SAP) in comparison with healthy non-atopic controls (HC). There was a significant higher baseline expression of CD69 on T cells from AAP and the difference was more notorious on CD161(+) T cells; upregulation of CD69 was observed on both CD161(-) and CD161(+) T cells driven by Dermatophagoides pteronyssinus crude extract, whereas polyclonal stimulation with phorbol 12-myristate 13-acetate plus ionomycin predominantly induced IFN-gamma but no IL-4, IL-5 and IL-13 by CD161(+) T cells in all groups; upon polyclonal stimulation, there were more CD161(+) T cells producing IFN-gamma and less CD161(-) T cells producing this cytokine, contrasting with the opposite results observed in SAP and HC groups. Our results indicate that, during asthma attack, CD161(+) T cells are activated and are able to produce predominantly IFN-gamma but no Th2 cytokines. We hypothesize that during an asthma attack, IFN-gamma produced by CD161(+) T cells could help to reestablish the Th1/Th2 equilibrium. These observations may contribute to the understanding of the immune mechanisms involved in asthma attacks.

  6. Analysis of basophil activation by flow cytometry in pediatric house dust mite allergy.

    Science.gov (United States)

    González-Muñoz, Miguel; Villota, Julian; Moneo, Ignacio

    2008-06-01

    Detection of allergen-induced basophil activation by flow cytometry has been shown to be a useful tool for allergy diagnosis. The aim of this study was to assess the potential of this technique for the diagnosis of pediatric house dust mite allergy. Quantification of total and specific IgE and basophil activation test were performed to evaluate mite allergic (n = 24), atopic (n = 23), and non-allergic children (n = 9). Allergen-induced basophil activation was detected as a CD63-upregulation. Receiver operating characteristics (ROC) curve analysis was performed to calculate the optimal cut-off value of activated basophils discriminating mite allergic and non-allergic children. ROC curve analysis yielded a threshold value of 18% activated basophils when mite-sensitized and atopic children were studied [area under the curve (AUC) = 0.99, 95% confidence interval (CI) = 0.97-1.01, p 43 kU/l) values for Dermatophagoides pteronyssinus allergen. They also showed positive prick (wheal diameter >1.0 cm) and basophil activation (>87%) tests and high specific IgE (>100 kU/l) with shrimp allergen. Shrimp sensitization was demonstrated by high levels of Pen a 1-specific IgE (>100 kU/l). Cross-reactivity between mite and shrimp was confirmed by fluorescence enzyme immunoassay (FEIA-CAP) inhibition study in these two cases. This study demonstrated that the analysis of allergen-induced CD63 upregulation by flow cytometry is a reliable tool for diagnosis of mite allergy in pediatric patients, with sensitivity similar to routine diagnostic tests and a higher specificity. Furthermore, this method can provide additional information in case of disagreement between in vivo and in vitro test results.

  7. Initial immunological changes as predictors for house dust mite immunotherapy response.

    Science.gov (United States)

    Gómez, E; Fernández, T D; Doña, I; Rondon, C; Campo, P; Gomez, F; Salas, M; Gonzalez, M; Perkins, J R; Palomares, F; Blanca, M; Torres, M J; Mayorga, C

    2015-10-01

    Although specific immunotherapy is the only aetiological treatment for allergic disorders, the underlying mechanisms are not fully understood. Specific immunotherapy induces changes in lymphocyte Th subsets from Th2 to Th1/Treg. Whether differences in immunological patterns underlie patient response to immunotherapy has not yet been established. We studied the immunological changes occurring during a 1-year period of Dermatophagoides pteronyssinus (DP) immunotherapy and their relation with clinical outcome. We included 34 patients with DP allergy who received subcutaneous specific immunotherapy (SCIT) for 1 year. Following treatment, patients were classified as responders or non-responders. Fourteen allergic subjects who did not receive SCIT were included as controls. Peripheral blood was obtained at 0, 1, 3, 6 and 12 months and cultured with nDer p 1. Phenotypic changes, cytokine production and basophil response were analysed by flow cytometry; transcription factors were measured by mRNA quantification. Serum immunoglobulin levels were also measured. After 1 year of SCIT, 82% of cases showed improved symptoms (responders). Although increases in sIgG4 were observed, BAT reactivity was not modified in these patients. Increases in T-BET/FOXP3 as well as nDer p 1-specific Th1/Treg frequencies were also observed, along with a decrease in Th2, Th9 and Th17. These changes corresponded to changes in cytokine levels. Patients who respond well to DP-SCIT show immunological differences compared to non-responders. In responders, basal differences include a lower frequency of Th1 and higher frequencies of Th2, Th9 and Th17 cells. After 1 year of treatment, an increased production of sIgG4 was observed in responders, along with a change in Th2 response towards Th1/Treg. © 2015 John Wiley & Sons Ltd.

  8. Occupational allergy to aquarium fish food: red midge larva, freshwater shrimp, and earthworm. A clinical and immunological study.

    Science.gov (United States)

    Meseguer Arce, J; Villajos, I M Sánchez-Guerrero; Iraola, V; Carnés, J; Fernández Caldas, E

    2013-01-01

    Chironomids seem to be the main cause of occupational allergy to aquarium fish food. The aim of this study was to investigate the pattern of occupational sensitization to 3 different arthropod species used as components of aquarium fish food. The study sample comprised 8 workers from a fish food packing department. The control group comprised 40 atopic patients (20 of whom were allergic to mites). We performed prick tests with extracts of red midge larva (Chironomus thummi), freshwater shrimp (Gammarus species), earthworm (Tubifex species), and other arthropod species and a battery of common inhalant allergens. We measured peak expiratory flow rate (PEFR) and specific immunoglobulin (Ig) E and performed a methacholine challenge test, nasal challenge test, and immunoblotting. Cross-reactivity analyses were completed using immunoblotting and CAP inhibition. Prick test results were positive to red midge larvae in 7 patients (87.5%), Gammarus in 5 (62.5%), Tubifex in 3 (37.5%), and mites in 6 (75%). In the mite-allergic controls, 30% had positive prick test results to red midge larvae. PEFR decreased > or = 20% during the packing process in all patients, and in 1 patient it indicated a dual asthmatic response. Methacholine challenge test results were positive in all participants. Nasal challenge tests were performed in 4 patients, and the results were positive. Specific IgE to red midge larvae was detected in 62.5%, Gammarus in 50%, and Tubifex in 16%. Bands of approximately 14-15 kDa and 31 kDa were observed in Gammarus and red midge larvae extracts. Cross-reactivity assays demonstrated that Gammarus totally inhibited red midge larvae, while Tubifex did so partially. Dermatophagoides pteronyssinus showed very low inhibitory capacity. Aquarium fish food arthropods are potent allergens with an elevated prevalence of sensitization and variable degree of crossreactivity. This is the first report of occupational allergy to Tubifex. More data are necessary to identify and

  9. Receptor for advanced glycation end products and its ligand high-mobility group box-1 mediate allergic airway sensitization and airway inflammation.

    Science.gov (United States)

    Ullah, Md Ashik; Loh, Zhixuan; Gan, Wan Jun; Zhang, Vivian; Yang, Huan; Li, Jian Hua; Yamamoto, Yasuhiko; Schmidt, Ann Marie; Armour, Carol L; Hughes, J Margaret; Phipps, Simon; Sukkar, Maria B

    2014-08-01

    The receptor for advanced glycation end products (RAGE) shares common ligands and signaling pathways with TLR4, a key mediator of house dust mite (Dermatophagoides pteronyssinus) (HDM) sensitization. We hypothesized that RAGE and its ligand high-mobility group box-1 (HMGB1) cooperate with TLR4 to mediate HDM sensitization. To determine the requirement for HMGB1 and RAGE, and their relationship with TLR4, in airway sensitization. TLR4(-/-), RAGE(-/-), and RAGE-TLR4(-/-) mice were intranasally exposed to HDM or cockroach (Blatella germanica) extracts, and features of allergic inflammation were measured during the sensitization or challenge phase. Anti-HMGB1 antibody and the IL-1 receptor antagonist Anakinra were used to inhibit HMGB1 and the IL-1 receptor, respectively. The magnitude of allergic airway inflammation in response to either HDM or cockroach sensitization and/or challenge was significantly reduced in the absence of RAGE but not further diminished in the absence of both RAGE and TLR4. HDM sensitization induced the release of HMGB1 from the airway epithelium in a biphasic manner, which corresponded to the sequential activation of TLR4 then RAGE. Release of HMGB1 in response to cockroach sensitization also was RAGE dependent. Significantly, HMGB1 release occurred downstream of TLR4-induced IL-1α, and upstream of IL-25 and IL-33 production. Adoptive transfer of HDM-pulsed RAGE(+/+)dendritic cells to RAGE(-/-) mice recapitulated the allergic responses after HDM challenge. Immunoneutralization of HMGB1 attenuated HDM-induced allergic airway inflammation. The HMGB1-RAGE axis mediates allergic airway sensitization and airway inflammation. Activation of this axis in response to different allergens acts to amplify the allergic inflammatory response, which exposes it as an attractive target for therapeutic intervention. Copyright © 2014 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

  10. Impairment of T-regulatory cells in cord blood of atopic mothers.

    Science.gov (United States)

    Schaub, Bianca; Liu, Jing; Höppler, Sabine; Haug, Severine; Sattler, Christine; Lluis, Anna; Illi, Sabina; von Mutius, Erika

    2008-06-01

    Maternal atopy is a strong predictor for the development of childhood allergic diseases. The underlying mechanisms are ill defined, yet regulatory T (Treg) and T(H)17 cells may play a key role potentially shaping the early immune system toward a proallergic or antiallergic immune regulation. We examined T(H)1/T(H)2, Treg, and T(H)17 cell responses to innate (lipid A/peptidoglycan) and mitogen/adaptive (phytohemagglutinin/Dermatophagoides pteronyssinus 1) immune stimulation in cord blood from offspring of atopic/nonatopic mothers. Cord blood mononuclear cells from 161 healthy neonates (59% nonatopic, 41% atopic mothers) were investigated regarding Treg and T(H)17 cells (mRNA/surface markers), suppressive function, and proliferation/cytokine secretion. Cord blood from offspring of atopic mothers showed fewer innate-induced Treg cells (CD4(+)CD25(+)high), lower mRNA expression of associated markers (glucocorticoid-induced tumor necrosis factor receptor-related protein/lymphocyte activation gene 3; P cell function was impaired in mitogen-induced suppression of T effector cells in cord blood of offspring from atopic mothers (P = .03). Furthermore, IL-10 and IFN-gamma secretion were decreased in innate-stimulated cord blood of offspring from atopic mothers (P = .04/.05). Innate-induced IL-17 was independent of maternal atopy and highly correlated with IL-13 secretion. In offspring of atopic mothers, Treg cell numbers, expression, and function were impaired at birth. T(H)17 cells were correlated with T(H)2 cells, independently of maternal atopy.

  11. Electrochemical aptasensor for detecting Der p2 allergen using polycarbonate-based double-generation gold nanoparticle chip

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    Ming-Che Shen

    2017-04-01

    Full Text Available In this study, a novel aptamer-based impedimetric biosensor for detecting the group 2 allergen of Dermatophagoides pteronyssinus (Der p2 was developed. First, an anodic aluminum oxide (AAO membrane was prepared. A modified AAO barrier-layer surface with an array of nanohemispheres of 400 nm in diameter was used as a template for the nanoelectroforming of a nickel mold. After electroforming, the AAO template was etched and a nickel nanomold with a concave nanostructure array was produced. The formed nanostructured nickel nanomold was then used in the replica molding of a nanostructured polycarbonate (PC substrate via hot embossing. Finally, a gold thin film was sputtered onto the PC substrate to form a double-generation gold nanoparticle electrode (array of nanohemispheres with smaller nanoparticles orderly distributed on each nanohemisphere. After immobilizing specifically designed aptamers on the fabricated electrode, electrochemical impedance spectroscopy was used to determine the concentration of Der p2. The sensitivity of the proposed scheme for the detection of the dust mite antigen Der p2 was 2.088 Ω / (ng/mL × cm2 with a dynamic detection range of 27.5–400 ng/mL and detection limit of 16.47 ng/mL.The aptamer-based impedimetric biosensor proposed in this study possesses many advantages such as high sensitivity, low cost, and high consistency over currently used sensors. The proposed sensor was found to be useful for the rapid detection of rare molecules present in an analyte. Keywords: Aptamers, Der p2 dust mite allergen detection, Nanostructured biosensors, Electrochemical impedance spectroscopy

  12. A survey of serum specific-lgE to common allergens in primary school children of Taipei City.

    Science.gov (United States)

    Wan, Kong-Sang; Yang, Winnie; Wu, Wei-Fong

    2010-03-01

    Environmental factors and eating habits have had a significant impact on the increased sensitization to allergens in children. This study investigated changes in common allergen sensitivities among children in Taipei City, Taiwan. A total of 142 primary schools in Taipei City, which included 25,094 students aged 7-8 years, were surveyed using an ISAAC questionnaire to screen for allergies. For positive responders, serum allergen-specific IgE was confirmed using the Pharmacia CAP system. A total of 1,500 students (5.98%) had confirmed sensitivities to allergens. Dust mite sensitivity among these children was nearly 90%. The prevalences of sensitivities to Dermatophagoides pteronyssinus, D. farinae and Blomia tropicalis were 90.79%, 88.24%, and 84.63%, respectively. Dog dander (29.95%) was the second most common aeroallergen to induce sensitivity. Allergies to cat dander (8.69%) and to cockroach (15.48%) had decreased dramatically compared with previous analyses. Among the food allergens studied, the most common allergens that induced sensitization were (in order of prevalence) crab, milk, egg white, and shrimp (88.08%, 22.45%, 24.23%, and 21.44%, respectively). Mold and pollen sensitization was identified in fewer than 2% of the schoolchildren. Dust mites remain the most common allergen to induce allergic sensitization among children in Taipei City, while cockroach and mold sensitivities have dramatically declined. Food allergens should also be considered as a trigger of respiratory allergy. Except for dust mites, American cockroach and crab, allergens commonly reported to induce sensitization in other Asian counties are not common in Taiwan.

  13. Allergen-stimulated T lymphocytes from allergic patients induce vascular cell adhesion molecule-1 (VCAM-1) expression and IL-6 production by endothelial cells.

    Science.gov (United States)

    Delneste, Y; Jeannin, P; Gosset, P; Lassalle, P; Cardot, E; Tillie-Leblond, I; Joseph, M; Pestel, J; Tonnel, A B

    1995-01-01

    Adhesion of inflammatory cells to endothelium is a critical step for their transvascular migration to inflammatory sites. To evaluate the relationship between T lymphocytes (TL) and vascular endothelium, supernatants from allergen-stimulated TL obtained from patients sensitive to Dermatophagoides pteronyssinus (Dpt) versus healthy subjects were added to endothelial cell (EC) cultures. TL were stimulated by autologous-activated antigen-presenting cells (APC) previously fixed in paraformaldehyde to prevent monokine secretion. Two parameters were measured: the expression of adhesion molecule and the production of IL-6. Related allergen-stimulated TL supernatants from allergic patients induced an increase of VCAM-1 and intercellular adhesion molecule-1 (ICAM-1) expression when supernatants of the control groups (TL exposed to an unrelated allergen or not stimulated or TL obtained from healthy subjects) did not. E-selectin expression was not modulated whatever the supernatant added to EC culture. IL-6 production by EC was significantly enhanced after activation with related allergen-stimulated TL supernatants from allergics compared with control supernatants. Induction of VCAM-1 expression was inhibited by adding neutralizing antibodies against IL-4, whereas IL-6 production and ICAM-1 expression were inhibited by anti-interferon-gamma (IFN-gamma) antibodies. Enhanced production of IL-4 and IFN-gamma was detected in related allergen-stimulated TL supernatants from allergic subjects compared with the different supernatants. These data suggest that allergen-specific TL present in the peripheral blood of allergic patients are of Th1 and Th2 subtypes. Their stimulation in allergic patients may lead to the activation of endothelial cells and thereby participate in leucocyte recruitment towards the inflammatory site. PMID:7542574

  14. Surfactant protein D attenuates sub-epithelial fibrosis in allergic airways disease through TGF-β.

    Science.gov (United States)

    Ogawa, Hirohisa; Ledford, Julie G; Mukherjee, Sambuddho; Aono, Yoshinori; Nishioka, Yasuhiko; Lee, James J; Izumi, Keisuke; Hollingsworth, John W

    2014-11-29

    Surfactant protein D (SP-D) can regulate both innate and adaptive immunity. Recently, SP-D has been shown to contribute to the pathogenesis of airway allergic inflammation and bleomycin-induced pulmonary fibrosis. However, in allergic airways disease, the role of SP-D in airway remodeling remains unknown. The objective of this study was to determine the contribution of functional SP-D in regulating sub-epithelial fibrosis in a mouse chronic house dust mite model of allergic airways disease. C57BL/6 wild-type (WT) and SP-D-/- mice (C57BL/6 background) were chronically challenged with house dust mite antigen (Dermatophagoides pteronyssinus, Dp). Studies with SP-D rescue and neutralization of TGF-β were conducted. Lung histopathology and the concentrations of collagen, growth factors, and cytokines present in the airspace and lung tissue were determined. Cultured eosinophils were stimulated by Dp in presence or absence of SP-D. Dp-challenged SP-D-/- mice demonstrate increased sub-epithelial fibrosis, collagen production, eosinophil infiltration, TGF-β1, and IL-13 production, when compared to Dp-challenged WT mice. By immunohistology, we detected an increase in TGF-β1 and IL-13 positive eosinophils in SP-D-/- mice. Purified eosinophils stimulated with Dp produced TGF-β1 and IL-13, which was prevented by co-incubation with SP-D. Additionally, treatment of Dp challenged SP-D-/- mice with exogenous SP-D was able to rescue the phenotypes observed in SP-D-/- mice and neutralization of TGF-β1 reduced sub-epithelial fibrosis in Dp-challenged SP-D-/- mice. These data support a protective role for SP-D in the pathogenesis of sub-epithelial fibrosis in a mouse model of allergic inflammation through regulation of eosinophil-derived TGF-β.

  15. Nuevas citas de ácaros oribátidos (Acari: Oribatida para la Argentina New records of oribatids (Acari: Oribatida for Argentina

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    Ana Salazar Martínez

    2006-12-01

    Full Text Available En la presente nota se citan, por primera vez para la Argentina , cuatro especies de ácaros oribátidos: Epilohmannia pallida americana Balogh & Mahunka, 1981, Protoribates (Triangius praeoccupatus (Pérez-Iñigo & Baggio, 1980, Scheloribates curvialatus Hammer, 1961 y Galumna innexa Pérez-Iñigo & Baggio, 1986. Los ejemplares fueron hallados en muestras de suelo recolectadas en La Plata , Provincia de Buenos Aires (34º 54'S, 57º 55´ W, en ambientes sometidos a intervención antrópica: bosques urbanos, huertas orgánicas y pastizales.Four species of oribatid mites are recorded from Argentina for the first time: Epilohmannia pallida americana Balogh & Mahunka, 1981, Protoribates (Triangius praeoccupatus (Pérez-Iñigo & Baggio, 1980, Scheloribates curvialatus Hammer, 1961 and Galumna innexa Pérez-Iñigo & Baggio, 1986. The specimens were collected in soil samples in La Plata , Buenos Aires Province (34º 54' S, 57º 55´ W in sites exposed to different degrees of human intervention: urban forest, organic orchards and pastures.

  16. The influence of sublethal deposits of agricultural mineral oil on the functional and numerical responses of Phytoseiulus persimilis (Acari: Phytoseiidae) to its prey, Tetranychus urticae (Acari: Tetranychidae).

    Science.gov (United States)

    Xue, Yingen; Meats, Alan; Beattie, G Andrew C; Spooner-Hart, Robert; Herron, Grant A

    2009-08-01

    Occasional pesticide application in integrated pest management to at least part of a crop requires that any biological control agents must re-invade previously sprayed areas in order that resurgent pests can be constrained. The ability of the phytoseiid predatory mite Phytoseiulus persimilis to feed on adult two-spotted spider mite (TSSM) Tetranychus urticae on excised leaf discs in both control conditions and in a treatment with a sub lethal residue of agricultural mineral oil (AMO) was assessed. The predator exhibited a Type II functional response with the asymptote significantly higher in the AMO conditions due to the fact that the prey grew slower and reached a smaller size in this treatment. In terms of prey volume eaten, the satiation level of the predator was unchanged by the AMO deposits. The numbers of eggs produced by adult P. persimilis females at densities of 4, 8 and 16 TSSM adult females/disc in the control were significantly higher than those in the AMO treatment, but were similar for the higher density levels, 32 and 64 prey per disc. Thus the functional response in terms of volume of prey eaten explained the numerical response in terms of predator eggs produced. The presence of AMO deposits when the prey were at high density had no effect on predator efficiency (volume eaten) but resulted in a lower intake than that in control conditions when there was a greater distance between prey.

  17. The effect of indoxacarb and five other insecticides on Phytoseiulus persimilis (Acari: Phytoseiidae), Amblyseius fallacis (Acari: Phytoseiidae) and nymphs of Orius insidiosus (Hemiptera: Anthocoridae).

    Science.gov (United States)

    Bostanian, Noubar J; Akalach, Mohammed

    2006-04-01

    A laboratory study assessed the contact toxicity of indoxacarb, abamectin, endosulfan, insecticidal soap, S-kinoprene and dimethoate to Amblyseius fallacis (Garman), Phytoseiulus persimilis Athias-Henriot and nymphs of Orius insidiosus (Say). Amblyseius fallacis is a predacious phytoseiid mite and an integral part of integrated pest management (IPM) programmes in North American apple orchards. The other two beneficials are widely used in greenhouses to manage various arthropod pests infesting vegetable and ornamental crops. Indoxacarb is a slow-acting insecticide, so toxicity data were recorded 7 days post-treatment when the data had stabilised. It showed no toxicity to O. insidiosus nymphs or to A. fallacis or P. persimilis adults. The LC50 values for O. insidiosus nymphs and P. persimilis could not be estimated with their associated confidence limits, because the g values were greater than 0.5 and under such circumstances the lethal concentration would lie outside the limits. The LC50 for A. fallacis was 7.6x the label rate. The fecundity of P. persimilis was reduced by 26.7%. The eclosion of treated eggs from both species of beneficial mites was not affected adversely. Among the other pest control products, S-kinoprene and endosulfan affected adversely at least one species of the predators, whereas dimethoate, abamectin and insecticidal soap were very toxic to all three beneficials. Indoxacarb should be evaluated as a pest control product in IPM programmes. Copyright (c) 2006 Crown in the right of Canada.

  18. The effect of chrysanthemum leaf trichome density and prey spatial distribution on predation of Tetranychus urticae (Acari: Tetranychidae) by Phytoseiulus persimilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Skirvin, D J; Stavrinides, M C; Skirvin, D J

    2003-08-01

    The effect of plant architecture, in terms of leaf hairiness, and prey spatial arrangement, on predation rate of eggs of the spider mite, Tetranychus urticae Koch, by the predatory mite Phytoseiulus persimilis Athias-Henriot was examined on cut stems of chrysanthemums. Three levels of leaf hairiness (trichome density) were obtained using two different chrysanthemum cultivars and two ages within one of the cultivars. The number of prey consumed by P. persimilis was inversely related to trichome density. At low prey densities (less than ten eggs per stem), prey consumption did not differ in a biologically meaningful way between treatments. The effect of prey spatial arrangement on the predation rate of P. persimilis was also examined. Predation rates were higher in prey patches on leaves adjacent to the release point of P. persimilis, but significantly greater numbers of prey were consumed in higher density prey patches compared to low density patches. The predators exhibited non-random searching behaviour, spending more time on leaves closest to the release point. The implications of these findings for biological control and predator-prey dynamics are discussed.

  19. Ritmo de queda de Rhipicephalus sanguineus (Acari: Ixodidae de cães artificialmente infestados Drop off rhythm of Rhipicephalus sanguineus (Acari: Ixodidae of artificially infested dogs

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    Gustavo F. Paz

    2008-09-01

    Full Text Available O ritmo de queda de Rhipicephalus sanguineus (Latreille foi avaliado para duas populações do Brasil; uma de Monte Negro, Estado de Rondônia, e outra de Belo Horizonte, Estado de Minas Gerais. Infestações artificiais com carrapatos das duas populações foram realizadas em cães domésticos mantidos em laboratório, sob o regime de luz: escotofase de 12:12 h. O padrão de queda de larvas ingurgitadas do hospedeiro se caracterizou pelo desprendimento de quantidades semelhantes de carrapatos em períodos de luz e de escotofase ou com um predomínio de queda durante o período de luz. No caso de ninfas e fêmeas ingurgitadas, a maioria dos carrapatos se desprendeu dos cães durante o período de escotofase, em todos os casos observados. Desta forma, é possível que em condições naturais, a maioria das ninfas e fêmeas adultas de R. sanguineus tendem a se desprender dos cães durante o período noturno, ao passo que larvas ingurgitadas se desprendem em proporções maiores durante o período diurno. Com base nestes dados, sugere-se que os locais onde os cães freqüentam durante a noite (onde possivelmente estará a maior parte da população de vida livre de R. sanguineus sejam priorizados com os tratamentos ambientais utilizando-se produtos carrapaticidas.The present study evaluated the drop-off rhythm of Rhipicephalus sanguineus (Latreille ticks from two populations from Brazil, one from Monte Negro, state of Rondônia, and another from Belo Horizonte, state of Minas Gerais. Artificial infestations with ticks were performed on dogs in the laboratory, held in a light: scotophase regimen of 12:12 h. Larval drop-off rhythm was characterized by similar number of engorged larvae detaching during both periods of light and scotophase, or by a larger number of larvae detaching during the light period. In contrast, most of the engorged nymphs and females detached from dogs during the scotophase period. These results indicate that under natural conditions, most of R. sanguineus engorged nymphs and females detach from dogs during the night period, whereas engorged larvae detach in higher proportions during daytime. Based on these data, tick control measures, encompassing environmental treatments with acaricide, should be indicated. The control measures are especially indicated in places where dogs spend or visit during the night period, since these places possibly harbor most of the free-living stages of R. sanguineus.

  20. A gallery of the key characters to ease identification of Dermanyssus gallinae (Acari: Gamasida: Dermanyssidae and allow differentiation from Ornithonyssus sylviarum (Acari: Gamasida: Macronyssidae

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    Di Palma Antonella

    2012-05-01

    Full Text Available Abstract Background Dermanyssus gallinae (poultry red mite is a major threat for the poultry industry and is of significant interest for public health. Identification of D. gallinae can be difficult for scientists not familiar with mite morphology and terminology especially when trying to use identification keys. Moreover, this species may easily be confused with another dermanyssoid mite, Ornithonyssus sylviarum (northern fowl mite, which often shares the same hosts and environment. Methods Specimens of D. gallinae were collected at poultry farms in the Puglia and performed for light and scanning electron microscopy observations, identification and micrographs. Moreover specimens of O. sylviarum were collected separately macerated and mounted on slides for light microscopy observations, identification and pictures. Results The micrographs used in this study, based on LM and SEM observations, highlight the following important identifying characters of D. gallinae: the prominent shoulders of the dorsal shield and the jagged edges of the shield reticulations, the position of setae j1, s1 and the epigynal pores, and the presence on tibia IV pl of one seta. Additional micrographs highlighting the shape of the dorsal (abruptly narrowed posteriorly and epigynal (narrowly rounded posteriorly shields and the chelicera (elongate, with distinct digits of O. sylviarum enable its differentiation from D.gallinae. Conclusion The photographic support provided here (both LM and SEM pictures can be considered a practical tool for scientists who are not well acquainted with the morphology of D.gallinae, and who are involved with classical and molecular systematics, veterinary and human health aspects of poultry red mites.

  1. Efeitos do nim sobre tetranychus urticae Koch (Acari: Tetranychidae e os predadores Phytoseiulus macropilis (Banks e Neoseiulus californicus (Mcgregor (Acari: Phytoseiidae

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    Daniele Cristine Hoffmann Schlesener

    2013-03-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficiência de controle e os efeitos adversos de dois produtos à base de nim Azamax® (Azadiractina A/B 12g/L e Neemseto® (Azadiractina A/B, Nimbina e Salanina 2,389 g/L sobre o ácaro-rajado Tetranychus urticae e os predadores Phytoseiulus macropilis e Neoseiulus californicus em laboratório. Para o ácaro-rajado, foram consideradas as variáveis mortalidade, fecundidade, efeito ovicida e persistência biológica, enquanto para os fitoseídeos consideraram-se mortalidade e fecundidade. A mortalidade máxima observada para o ácaro-rajado foi de 89,7% e 91,5% para Azamax® e Neemseto®, respectivamente, na concentração de 0,5% após a reaplicação do produto no sétimo dia. Também foram observados efeitos adversos sobre a fecundidade e a viabilidade dos ovos quando tratados com os produtos comerciais (p.c.. A persistência biológica dos produtos foi de aproximadamente três dias após a pulverização. As formulações apresentaram seletividade em relação aos fitoseídeos, porém causaram redução da fecundidade dos mesmos.

  2. Suscetibilidade de Oligonychus yothersi (Acari: Tetranychidae ao fungo Beauveria bassiana Susceptibility of Oligonychus yothersi (Acari: Tetranychidae to the fungus Beauveria bassiana

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    Renato Cassol de Oliveira

    2002-03-01

    Full Text Available A cultura da erva-mate (Ilex paraguariensis é uma importante atividade econômica no Sul do Brasil. Com o aumento de consumo nos anos oitenta, cresceram também as áreas de monocultura, gerando condições favoráveis para o aumento populacional de insetos e ácaros fitófagos, entre eles o ácaro vermelho Oligonychus yothersi. Este estudo avaliou a suscetibilidade do ácaro vermelho O. yothersi a vários isolados do fungo Beauveria bassiana. O experimento foi conduzido em Cascavel, PR. Discos foliares de erva-mate (2,2 cm² foram previamente infestados com 20 fêmeas adultas. Alíquotas de 1 mL de cada suspensão de conídios dos diferentes isolados de B. bassiana, ajustadas na concentração de 1,0x10(8 conídios mL-1 foram pulverizadas, separadamente, sobre um conjunto de 5 discos. Os discos foliares foram mantidos flutuando em água destilada, em caixas plásticas (3 cm de diâmetro, sob condições controladas. Diariamente, os ácaros mortos foram transferidos para câmara úmida, para confirmação de mortalidade causada pelo fungo. A mortalidade total variou entre 77 e 98% (6 dias após a aplicação, não permitindo diferenciar os isolados quanto à virulência (P>0,05. Já a mortalidade confirmada variou entre 19 e 75%, permitindo diferenciá-los (PThe Paraguay tea (Ilex paraguariensis crop is an important economical activity in Southern Brazil. With the increase of tea consumption in the 80s, the number of monoculture crops also increased, creating favorable conditions for the development of phytophagous insect and mite populations, such as the red mite Oligonychus yothersi. The objective of this study was to evaluate the susceptibility of the red mite O. yothersi to the several strains of the Beauveria bassiana fungus. The experiments were carried out in Cascavel, PR, Brazil. Leaf disks of Paraguay tea (2.2 cm² were previously infested with 20 adult females. Five disks were inoculated with 1 mL of each conidial suspension of B. bassiana strains (1x10(8 conidia per mL. Disks were kept afloat on distilled water in plastic boxes (3 cm diameter, under controlled conditions. Dead mites were transferred to a humid chamber on a daily basis to confirm the mortalities rate by the fungus. All the strains were pathogenic, with total mortality varying from 77 to 98%, 6 days after application. It was not possible to differentiate virulence levels between strains (P<0.05 by total mortality. However, confirmed mortality varied from 19 to 75%, 8 days after application, allowing for a differentiation in virulence levels between strains (P<0.05. This study concluded that the O. yothersi red mite is susceptible, at different levels, to B. bassiana tested strains.

  3. Environmentally associated ticks (Acari: Ixodidae in Campo Grande, Mato Grosso do Sul, Brazil Carrapatos (Acari: Ixodidae associados com o ambiente em Campo Grande, Mato Grosso do Sul, Brasil

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    Marcos Valério Garcia

    2013-03-01

    Full Text Available Herein, we report tick species found on wild and domestic animals and in the environment during a one-year sampling period at the Brazilian Farming Research Company beef cattle unit (Embrapa Beef Cattle, which is located within the urban area of Campo Grande, Mato Grosso do Sul, Brazil. From 55 wild hosts including six different species (Nasua nasua, Cebus spp., Cerdocyon thous, Myrmecophaga tridactyla, Tamandua tetradactyla and Dasyprocta aguti, 323 ticks were collected. Amblyomma ovale ticks were found solely on coatis, and Amblyomma nodosum was identified solely on anteaters. No ticks were found on capuchin monkeys. However, Amblyomma cajennense was found on all parasitized host species with the exception of capuchin monkeys. Giant anteaters displayed the highest infestation abundance, with a mean of 53 ticks∕animal. Environmental sampling yielded 166 adult A. cajennense ticks. The tick species found on domestic animals (Rhipicephalus (Boophilus microplus, R. sanguineus, Dermacentor nitens and A. cajennense were those typically found on these hosts in Brazil. The most prevalent tick species, A. cajennense, was found on both wild and domestic animals and was also prevalent in the environment. Thus, this tick species is the primary vector that allows pathogens to bridge wild and domestic animals in the Cerrado.Neste trabalho são descritas as espécies de carrapatos de animais selvagens e domésticos e do ambiente coletados por um ano na EMBRAPA Gado de Corte localizado na área urbana de Campo Grande, Mato Grosso do Sul, Brasil. Dos 55 hospedeiros selvagens de seis espécies diferentes (Nasua nasua, Cebus spp., Cerdocyon thous, Myrmecophaga tridactyla, Tamandua tetradactyla e Dasyprocta aguti foram coletados 323 carrapatos. Amblyomma ovale foi encontrado apenas em quatis e Amblyomma nodosum apenas sobre tamanduás. Nenhum carrapato foi encontrado sobre macacos-prego. Por outro lado, Amblyomma cajennense foi encontrado em todos os hospedeiros com exceção dos macacos-pregos. A maior abundancia de infestação foi aquela em tamanduás-bandeira com média de 53 carrapatos∕animal. No ambiente foram capturados 166 carrapatos, todos da espécie A. cajennense. As espécies de carrapatos em animais domésticos (Rhipicephalus (Boophilus microplus, Rhipicephalus sanguineus, Dermacentor nitens e A. cajennense foram aquelas características nestes hospedeiros no Brasil. De forma geral a espécie de carrapato A. cajennense foi a mais prevalente sendo encontrada em animais domésticos e selvagens bem como no ambiente. Portanto, esta é a principal espécie de vetor a estabelecer uma ponte para bioagentes patogênicos entre animais domésticos e selvagens.

  4. Selection of Entomopathogenic Fungi to Control Varroa destructor (Acari: Varroidae Selección de Hongos Entomopatógenos para el Control de Varroa destructor (Acari: Varroidae

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    Marta Rodríguez

    2009-12-01

    Full Text Available The aim of this work was select entomopathogenic fungi tolerant to temperatures inside the brood area of honey bees (Apis mellifera for to control Varroa destructor. For this purpose, 50 Beauveria bassiana (Balsamo Vuillemin and 48 Metarhizium anisopliae (Metschn. Sorokin isolates were evaluated at 30 and 35 ºC. For each isolate, colony discs of 5 mm with mycelium were placed in the center of a Petri dish with Sabouraud dextrose agar (SDA medium. The dishes were incubated at 30 and 35 °C, without light. Radial growth of each colony was measured daily. All the B. bassiana and M. anisopliae isolates presented a lineal growth rate at a temperature of 30 ºC. However, at 35 ºC, most of the isolates did not grow, except three B. bassiana and 14 M. anisopliae isolates (P El objetivo de este trabajo fue seleccionar hongos entomopatógenos tolerantes a las temperaturas del nido de cría de las abejas (Apis mellifera, para ser utilizados en el control de Varroa destructor. Se evaluaron 50 aislamientos de Beauveria bassiana (Balsamo Vuillemin y 48 de Metarhizium anisopliae (Metschn. Sorokin a temperaturas de 30 y 35 ºC. Discos de agar de 5 mm de diámetro con micelio de colonias de cada aislamiento, se depositaron en el centro de placas Petri con medio agar Sabouraud dextrosa (ASD. Las placas fueron incubadas a 30 y 35 °C y oscuridad y diariamente se midió el radio de cada colonia. Todos los aislamientos de B. bassiana y M. anisopliae var. anisopliae presentaron una tendencia lineal a través del tiempo a temperaturas de incubación de 30 °C. A 35 °C la mayoría de los aislamientos no crecieron, excepto tres aislamientos de B. bassiana y 14 de M. anisopliae (p < 0,001. Estos aislamientos fueron seleccionados para realizar pruebas de patogenicidad sobre V. destructor, aplicando una suspensión de 10(7 conidias mL-1. El aislamiento más efectivo fue Qu-M845 de M. anisopliae (p = 0,0033, produjo una mortalidad de 85%. La capacidad patogénica de este aislamiento en V. destructor y su tolerancia a las condiciones ambientales a las que sería expuesto, permite considerar esta cepa como una alternativa de control para esta plaga.

  5. Fitoseídeos (Acari: Phytoseiidae associados a cafezais e fragmentos florestais vizinhos Phytoseiids (Acari: Phytoseiidae associated to coffee plantations and adjacent forest fragments

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    Ester Azevedo Silva

    2010-10-01

    Full Text Available Existem poucas informações sobre a fauna de ácaros predadores (Phytoseiidae em ambientes naturais brasileiros adjacentes a agroecossistemas cafeeiros (Coffea spp. ou sobre a influência que essa vegetação exerce como reservatório de ácaros predadores. Neste estudo, objetivou-se avaliar a diversidade destes organismos em cafeeiros e fragmentos florestais adjacentes. Coletaram-se amostras das espécies Calyptranthes clusiifolia (Miq. O. Berg (Myrtaceae, Esenbeckia febrifuga (A. St.-Hil. A. Juss. ex Mart. (Rutaceae, Metrodorea stipularis Mart. (Rutaceae e Allophylus semidentatus (Miq. Radlk. (Sapindaceae, em oito fragmentos florestais, de 5 a 51 ha, e cafezais adjacentes, nos meses de junho (final período chuvoso e outubro (final período seco nos anos 2004 e 2005, na região Sul do Estado de Minas Gerais. Ácaros foram extraídos das folhas, utilizando o método de lavagem e, em seguida, montados em lâminas de microscopia em meio de Hoyer, para identificação específica. No total foram identificados 2.348 fitoseídeos, sendo 2.090 nos fragmentos florestais e 258 espécimes nos cafezais adjacentes, pertencentes a 38 espécies. Servindo-se de análise faunística, a espécie Iphiseiodes zuluagai Denmark & Muma, 1972 apresentou os melhores índices no agroecossistema cafeeiro, sendo muito frequente e constante nas épocas estudadas. Nos fragmentos florestais Amblyseius herbicolus Chant, 1959, Iphiseiodes affs. neonobilis Denmark & Muma, 1978, Leonseius regularis DeLeon, 1965 e Euseius alatus DeLeon, 1966 foram dominantes, muito abundantes, muito frequentes e constantes nas épocas estudadas. Podemos concluir que a vegetação nativa abriga ácaros predadores, inimigos naturais de ácaros-praga, que ocorrem na cultura cafeeira, possibilitando o desenvolvimento de programas de manejo ecológico com áreas de vegetação natural e agroecossistemas cafeeiros adjacentes.There is little information about the fauna of predatory mites (Phytoseiidae in Brazilian natural environments, adjacent to coffee agroecosystems (Coffea spp., or about the influence exerted by neighbor vegetation as a reservoir of predatory mites. The objective of this study was to evaluate the diversity of these organisms in coffee plantations and adjacent forest fragments. Samples of the species Calyptranthes clusiifolia (Miq. O. Berg (Myrtaceae, Esenbeckia febrifuga (A. St.-Hil. A. Juss. ex Mart., Metrodorea stipularis Mart. (Rutaceae and Allophylus semidentatus (Miq. Radlk. (Sapindaceae were collected in eight forest fragments, from 5 to 51ha, adjacent to coffee plantations, in June (end of the rainy season and October (end of the dry season in the years of 2004 and 2005, in the Southern region of State of Minas Gerais. Leaf mites were extracted using the wash method, mounted in microscopy slides with Hoyer's medium for identification. A total of 2.348 phytoseiids was collected, being 2.090 in the forest fragments and 258 in adjacent coffee plantations, belonging to 38 species. According to fauna analysis, Iphiseiodes zuluaguai Denmark & Muma, the year of 1972 presented the best indexes in the coffee agroecosystem, being very frequent and constant in those periods. In the forest fragments, Amblyseius herbicolus Chant, 1959, Iphiseiodes affs. neonobilis Denmark & Muma, 1978, Leonseius regularis DeLeon, 1965 and Euseius alatus DeLeon, 1966 were dominant, very abundant, very frequent and constant in those periods. One may conclude that the native vegetation shelters predator mite, natural enemies of mite-pests that still occur in coffee culture, making possible ecological management program development involving areas of natural vegetation and adjacent coffee agroecosystems.

  6. Distribuição espacial e plano de amostragem de Calacarus heveae (Acari em seringueira Spatial distribution and sampling plan for Calacarus heveae (Acari on rubber trees

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    Noeli Juarez Ferla

    2007-12-01

    Full Text Available Calacarus heveae Feres, 1992 é um eriofídeo descrito de espécimes coletados em plantas de seringueira (Hevea brasiliensis, Euphorbiaceae na região noroeste do Estado de São Paulo. Esse ácaro prefere a face adaxial dos folíolos e pode causar a perda do brilho, amarelecimento, bronzeamento dessa região e a subseqüente queda prematura das folhas. O objetivo deste trabalho foi analisar a distribuição de C. heveae em seringueira, selecionar a unidade de amostragem mais representativa e desenvolver um plano de amostragem para o estudo de sua flutuação populacional. O trabalho foi conduzido com os clones PB 260 e IAN 873, respectivamente nos municípios de Itiquira e de Pontes e Lacerda, ambos no Mato Grosso. Em Itiquira, diferenças significativas foram observadas em quatro ocasiões em relação ao número médio de ácaros por folha nos diferentes estratos das plantas. Nas amostragens realizadas em Pontes e Lacerda, nenhuma diferença significativa foi encontrada entre os estratos em relação àquele parâmetro. Apenas em Itiquira, em uma ocasião de amostragem, foi verificada diferença entre os três estratos, em relação à proporção de folhas infestadas. Nenhuma diferença significativa foi verificada em relação ao número médio de ácaros por folha e proporção de folhas infestadas por C. heveae a diferentes distâncias da periferia da copa. Calacarus heveae exibe distribuição agregada no campo. Para estimar a densidade de C. heveae, um plano numérico e um plano binomial de amostragem foram desenvolvidos.Calacarus heveae Feres, 1992 (Eriophyidae is a mite described from specimens collected on rubber tree (Hevea brasiliensis, Euphorbiaceae in the northwest region of the state of São Paulo. This mite prefers the adaxial face of the folioles which it can turn dry, yellowish and brownish; it can also cause leaf fall. The aim of this work was to analyze the distribution of C. heveae on rubber trees, to select the most representative sampling unit and to develop a sampling plan to determine the populational fluctuation. This study was conducted with clones PB 260 and IAN 873, in Itiquira and Pontes e Lacerda, respectively, both in the state of Mato Grosso. In Itiquira, significant differences were observed in four occasions in relation to the average number of mites per leaf in the different plant strata. In the samplings carried out in Pontes e Lacerda, no significant differences were observed between strata in relation to that parameter. Only in Itiquira, in one occasion, a significant difference between strata was verified in relation to the proportion of infested leaves. No significant differences were verified in relation to the average number of mites per leaf and proportion of leaves infested by C. heveae at different depths in the canopy. Calacarus heveae exhibits aggregated distribution in the field. To estimate the density of C. heveae, numeric and sampling plans were developed.

  7. Nuevos registros de ácaros oribátidos (Acari: Oribatida para la Argentina New records of oribatid mites (Acari: Oribatida for Argentina

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    Cecilia Accattoli

    2010-12-01

    Full Text Available Esta publicación constituye un aporte al conocimiento del elenco oribatológico en suelos de Argentina, basado en relevamientos realizados en un parque urbano de la ciudad de La Plata, Buenos Aires. Se informan dos nuevas citas de géneros, una de subgénero y siete de especies para el país. Además, cinco de las especies halladas serían nuevas para la ciencia. Se incorporan ocho géneros y siete especies a las registradas para la Provincia de Buenos Aires.This paper is a contribution to the knowledge of assemblages of oribatid mites in soils of Argentina. The study is based on samples from an urban forest in the city of La Plata, Buenos Aires. New records for the country are provided for two genera, one subgenus and seven species. Furthermore, five of the species found are possibly new to science. Eight genera and seven species are incorporated to the record of the Buenos Aires province.

  8. CHECKLIST OF THE ERIOPHYOID MITE FAUNA OF MONTENEGRO (ACARI: PROSTIGMATA: ERIOPHYOIDEA

    OpenAIRE

    Jočić, Ivona; Petanović, Radmila

    2012-01-01

    Accounts are given of the eriophyoid fauna of Montenegro. Based on the literature records, a total of 156 mite species are listed from 130 host species of 42 plant families. The families Phytoptidae, Eriophidae and Diptilomiopidae are represented by 6, 138 and 12 species, respectively. Fifteen new species for science have been described from this area.

  9. New and little known species of ptyctimous mites (Acari, Oribatida) from Cameroon

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2014-01-01

    Roč. 3889, č. 1 (2014), s. 31-57 ISSN 1175-5326 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : ptyctimous mites * new species * taxonomy * morphology * Cameroon Subject RIV: EG - Zoology Impact factor: 0.906, year: 2014

  10. New and little known species of ptyctimous mites (Acari, Oribatida) from Costa Rica

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2014-01-01

    Roč. 40, č. 4 (2014), s. 320-327 ISSN 0164-7954 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Oribatida * ptyctimous mites * taxonomy * descriptions * tritonymph Subject RIV: EG - Zoology Impact factor: 0.949, year: 2014

  11. Diversity and significance of eriophyoid mites (Acari: Eriophyoidea associated with coniferous trees in Poland: a review

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    Kiedrowicz Agnieszka

    2016-06-01

    Full Text Available Among the approximately 200 eriophyoid mite species associated with coniferous trees worldwide, 33 species (of the families Eriophyidae and Phytoptidae infest conifers in Poland, and 24 of them can cause visible feeding symptoms. In this paper we discuss the importance of eriophyoid mites to coniferous plants in Poland and their potential impact on the decorative value of ornamental plants. We emphasize the general lack of knowledge about the diversity of eriophyoid mites associated with coniferous trees and its role in the management and control of this economically important mite group.

  12. Detection of Leishmania (V guyanensis in Rhipicephalus (Boophilus microplus (Acari: Ixodidae collected from Pecari tajacu

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    Jesús E. Rojas-Jaimes

    2017-03-01

    Conclusion: The results showed the presence of L. (V guyanensis DNA in R. microplus possibly acquired after biting a collarde peccary. Therefore, it is important to design future studies to clarify R. microplus involvement in the transmission of leishmaniasis.

  13. Preliminary survey of ticks (Acari : Ixodidae on cattle in northern Sudan

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    D.A. Salih

    2004-11-01

    Full Text Available In a cross sectional survey conducted during the period June 2001 to July 2002, the geographical distribution of ticks on cattle in the Sudan was determined. Seventeen locations were surveyed from Northern, Central, Eastern, Western, Blue Nile and White Nile Provinces. Total body collections of ticks were made from 20 cattle at each location. Four tick genera and 11 species were identified. The tick species collected included Amblyomma lepidum, Amblyomma variegatum, Boophilus decoloratus, Hyalomma anatolicum anatolicum, Hyalomma dromedarii, Hyalomma impeltatum, Hyalomma marginatum rufipes, Hyalomma truncatum, Rhipicephalus evertsi evertsi, Rhipicephalus sanguineus group and Rhipicephalus simus simus. Major ecological changes have occurred due to extensive animal movement, deforestation, desertification and establishment of large mechanized agricultural schemes. These factors have certainly affected the distribution of ticks and tick-borne diseases in the Sudan. The absence of A. variegatum and A. lepidum in northern Sudan was not surprising, since these tick species are known to survive in humid areas and not in the desert and semi-desert areas of northern Sudan. The absence of B. annulatus in northern and central Sudan is in accordance with the finding that this tick species is restricted to the southern parts of the central Sudan. The presence of H. anatolicum anatolicum in Um Benin in relatively high abundance is an interesting finding. The present finding may indicate that the southern limit of this species has changed and moved southwards to latitude 13o N. It is concluded that major changes in tick distribution have taken place in the Sudan

  14. Toxicity and efficacy of selected pesticides and new acaricides to stored product mites (Acari : Acaridida)

    Czech Academy of Sciences Publication Activity Database

    Hubert, J.; Stejskal, V.; Münzbergová, Zuzana; Hajšlová, J.; Arthur, H. F.

    2007-01-01

    Roč. 42, č. 4 (2007), s. 283-290 ISSN 0168-8162 Grant - others:GA MŠk(CZ) 2B06099 Institutional research plan: CEZ:AV0Z60050516 Keywords : allergens * pesticide s * food Subject RIV: EF - Botanics Impact factor: 1.260, year: 2007

  15. Divergent methylation pattern in adult stage between two forms of Tetranychus urticae (Acari: Tetranychidae).

    Science.gov (United States)

    Yang, Si-Xia; Guo, Chao; Zhao, Xiu-Ting; Sun, Jing-Tao; Hong, Xiao-Yue

    2017-02-19

    The two-spotted spider mite, Tetranychus urticae Koch has two forms: green form and red form. Understanding the molecular basis of how these two forms established without divergent genetic background is an intriguing area. As a well-known epigenetic process, DNA methylation has particularly important roles in gene regulation and developmental variation across diverse organisms that do not alter genetic background. Here, to investigate whether DNA methylation could be associated with different phenotypic consequences in the two forms of T. urticae, we surveyed the genome-wide cytosine methylation status and expression level of DNA methyltransferase 3 (Tudnmt3) throughout their entire life cycle. Methylation-sensitive amplification polymorphism (MSAP) analyses of 585 loci revealed variable methylation patterns in the different developmental stages. In particular, principal coordinates analysis (PCoA) indicates a significant epigenetic differentiation between female adults of the two forms. The gene expression of Tudnmt3 was detected in all examined developmental stages, which was significantly different in the adult stage of the two forms. Together, our results reveal the epigenetic distance between the two forms of T. urticae, suggesting that DNA methylation might be implicated in different developmental demands, and contribute to different phenotypes in the adult stage of these two forms. © 2017 Institute of Zoology, Chinese Academy of Sciences.

  16. Two new Anoplocheylus species (Acari: Trombidiformes: Pseudocheylidae) from Kurdistan province of Iran.

    Science.gov (United States)

    Khanjani, Mohammad; Hoseini, Mohammad Ahmad; Amini, Fatemeh

    2014-09-12

    Two new species of the genus Anoplocheylus Berlese, 1910 are described: Anoplocheylus marivaniensis sp. nov. collected from soil and rotten leaves under oak trees and Anoplocheylus qorvehiensis sp. nov. from soil under Astragalus sp. bushes in Kurdistan province, Iran. A key to females of all known species of Anoplocheylus is provided, based on original descriptions and other literature.

  17. How do Neoseiulus californicus (Acari: Phytoseiidae) females penetrate densely webbed spider mite nests?

    Science.gov (United States)

    Montserrat, M; de la Peña, F; Hormaza, J I; González-Fernández, J J

    2008-02-01

    The persea mite Oligonychus perseae is a pest of avocado trees that builds extremely dense webbed nests that protect them against natural enemies, including phytoseiid mites. Nests have one or two marginal entrances that are small and flattened. The predatory mite Neoseiulus californicus co-occurs with O. perseae in the avocado orchards of the south-east of Spain. Penetration inside nests through the entrances by this predator is thought to be hindered by its size and its globular-shaped body. However, in the field it has repeatedly been found inside nests that were clearly ripped. Perhaps penetration of the nests has been facilitated by nest wall ripping caused by some other species or by unfavourable abiotic factors. However, to assess whether N. californicus is also able to enter the nest of O. perseae by itself, we carried out laboratory experiments and made a short film. They show how this predator manages to overcome the webbed wall, and that it can penetrate and forage inside nests of O. perseae.

  18. A new species of the genus Epidamaeus (Acari, Oribatida, Damaeidae from China

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    Lixia Xie

    2011-07-01

    Full Text Available The oribatid mite genus Epidamaeus Bulanova-Zachvatkina, 1957 from China is reviewed, and a list and key to all known species from China are provided. A new species, Epidamaeus conjungenus, sp. n. is identified, and its morphological descriptions and illustrations are also given. The distinct characteristics of E. conjungenus sp. n. is the coterminous ridge connected to the base of the notogastral setae. Pseudanal setae undulating attenuate, the proximal half with obvious, thorn-like barbs, the distal half smooth.

  19. Complementary data on Haplozetes fusifer (Berlese, 1908 (Acari, Oribatida, Haplozetidae collected from Iran

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    Akrami, M. A.

    2013-12-01

    Full Text Available An oribatid mite of the family Haplozetidae, Haplozetes fusifer (Berlese, 1908 is redescribed on the basis of Iranian materials, including the first detailed descriptions of the gnathosoma and legs.Se redescribe un ácaro oribátido de la familia Haplozetidae, Haplozetes fusifer (Berlese, 1908, sobre la base de material iraní, incluyendo las primeras descripciones detalladas del gnatosoma y las patas.

  20. New and little known species of ptyctimous mites (Acari, Oribatida) from Madagascar

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2016-01-01

    Roč. 4103, č. 6 (2016), s. 587-599 ISSN 1175-5326 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : taxonomy * ptyctimous mites * new species * morphology * Madagascar Subject RIV: EG - Zoology Impact factor: 0.972, year: 2016

  1. Two new species and new records of chiggers (Acari: Leeuwenhoekiidae,Trombiculidae) from birds in Vietnam

    Czech Academy of Sciences Publication Activity Database

    Kalúz, S.; Hung, N. M.; Čapek, Miroslav; Literák, I.

    2016-01-01

    Roč. 4061, č. 5 (2016), s. 483-503 ISSN 1175-5326 Institutional support: RVO:68081766 Keywords : birds * chiggers * taxonomy * Leptotrombidium * Neoschoengastia * Hypogastia * new species Subject RIV: EG - Zoology Impact factor: 0.972, year: 2016

  2. Soil mites of the families Ascidae, Blattisociidae and Melicharidae (Acari: Mesostigmata) from mountainous areas of Colombia.

    Science.gov (United States)

    Rueda-Ramírez, Diana; Varela, Amanda; Moraes, Gilberto J De

    2016-06-24

    Soil mites of the Ascidae sensu Lindquist & Evans (1965) are poorly known in Colombia. This group, presently represented by the families Ascidae sensu stricto, Blattisociidae and Melicharidae, contains species known to prey on small arthropods and nematodes, thus having the potential to be used for the control of soil pests. The aim of this study was to identify species of this group from a fragment of Andean forest and a nearby grassland at the municipality of La Calera, Cundinamarca Department, Colombia, at about 2800 m of elevation. Nine species were found, including five new species, namely Gamasellodes andinus sp. nov., Gamasellodes intermedius sp. nov., Protogamasellus caleraensis sp. nov., Cheiroseius mesae sp. nov. and Proctolaelaps colombianus sp. nov. Morphological characterisation of all the species and relevant soil characteristics of the sites where the mites were collected are presented.

  3. Reproductive performance of the generalist predator Hypoaspis aculeifer (Acari: Gamasida) when foraging on different invertebrate prey

    DEFF Research Database (Denmark)

    Heckmann, Lars-Henrik; Ruf, A.; Nienstedt, K. M.

    2007-01-01

    (Caloglyphus cf. Michaeli), an oligochaete (Enchytraeus crypticus), a nematode (Turbatrix silusiae), and a 1:1:1 mix of F. candida : F. fimetaria : E. crypticus. Our results revealed that a single prey species may be nutritionally sufficient for a 3-week period, as H. aculeifer performed equally well......, or better, on a diet based on a 1:1:1 mix of F. candida : F. fimetaria : E. crypticus. However, when fed C. cf. michaeli, H. aculeifer had a poor reproductive output (... performance during toxicant exposure....

  4. Transstadial Transmission of Hepatozoon canis by Rhipicephalus sanguineus (Acari: Ixodidae) in Field Conditions.

    Science.gov (United States)

    Aktas, M; Özübek, S

    2017-07-01

    This study investigated possible transovarial and transstadial transmission of Hepatozoon canis by Rhipicephalus sanguineus (Latreille) ticks collected from naturally infected dogs in a municipal dog shelter and the grounds of the shelter. Four hundred sixty-five engorged nymphs were collected from 16 stray dogs that were found to be infected with H. canis by blood smear and PCR analyses and maintained in an incubator at 28 °C for moulting. Four hundred eighteen nymphs moulted to adults 14-16 d post collection. Unfed ticks from the shelter grounds comprised 1,500 larvae, 2,100 nymphs, and 85 adults; were sorted according to origin, developmental stage, and sex into 117 pools; and screened by 18S rRNA PCR for Hepatozoon infection. Of 60 adult tick pools examined, 51 were infected with H. canis. The overall maximum likelihood estimate (MLE) of infection rate was calculated as 21.0% (CI 15.80-28.21). Hepatozoon canis was detected in 31 out of 33 female pools (MLE 26.96%, CI 17.64-44.33) and 20 out of 27 male pools (MLE 14.82%, CI 20.15-46.41). Among 42 unfed nymph pools collected from the shelter, 26 were infected with H. canis, and MLE of infection was calculated as 1.9% (CI 1.25-2.77). No H. canis DNA was detected in any of the gDNA pools consisting of larva specimens. Partial sequences of the 18S rRNA gene shared 99-100% similarity with the corresponding H. canis isolates. Our results revealed the transstadial transmission of H. canis by R. sanguineus, both from larva to nymph and from nymph to adult, in field conditions. However, there were no evidence of transovarial transmission. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  5. Acquisition and transmission of Hepatozoon canis (Apicomplexa: Hepatozoidae) by the tick Amblyomma ovale (Acari: Ixodidae).

    Science.gov (United States)

    Rubini, A S; Paduan, K S; Martins, T F; Labruna, M B; O'Dwyer, L H

    2009-10-14

    The present study aimed to evaluate under controlled conditions the acquisition of Hepatozoon canis by Amblyomma ovale after feeding on infected dogs, and the subsequent induction of infection in uninfected dogs that ingested the experimentally infected ticks. Two H. canis naturally infected dogs were infested with A. ovale adult ticks derived from an uninfected laboratory tick colony. After feeding, two A. ovale females presented H. canis oocysts in the hemolymph at the first and fourth days after removal of ticks from dogs. The oocysts had an average size of 244.34 microm x 255.46 microm. Three uninfected dogs were fed with ticks previously fed on the infected dogs. Only one dog became infected 32 days after oral inoculation, presenting circulating gametocytes, parasitemia less than 1%, and positive PCR confirmed to be H. canis by DNA sequencing. The results obtained indicated A. ovale ticks as potential vector of H. canis in rural areas of Brazil.

  6. Demodex injai: a new species of hair follicle mite (Acari: Demodecidae) from the domestic dog (Canidae).

    Science.gov (United States)

    Desch, Clifford E; Hillier, Andrew

    2003-03-01

    Demondex injai sp. nov. is described from the hair follicles of a domestic dog in Columbus, OH in October 1996. The mites occupy follicles from the orifice down to and into the sebaceous glands. The individual host may harbor both this new species and D. canis. A comparison of these two species is provided for identification purposes.

  7. A New Stubby Species of Demodectic Mite (Acari: Demodicidae) From the Domestic Dog (Canidae).

    Science.gov (United States)

    Morita, Tatsushi; Ohmi, Aki; Kiwaki, Akihito; Ike, Kazunori; Nagata, Katsuyuki

    2018-02-28

    A new species of Demodex was detected in the earwax of a dog with otitis externa in Saitama Prefecture, Japan, in July 2010. The opisthosoma length of the mite was slightly shorter than 1/2 of its body length, which was different from the other species in domestic dogs, D. canis and D. injai, but was similar to the form of mites termed "short-bodied species", including D. cornei. However, the stubby external form was morphologically different from those of "short-bodied species", excluding a case without a species description reported from Greece. Among known species, the mite was similar to D. equi and D. acutipes.

  8. RAPD-SCAR marker and genetic relationship analysis of three Demodex species (Acari: Demodicidae).

    Science.gov (United States)

    Zhao, Ya-E; Wu, Li-Ping

    2012-06-01

    For a long time, classification of Demodex mites has been mainly based on their hosts and phenotype characteristics. The study was the first to conduct molecular identification and genetic relationship analysis for six isolates of three Demodex species by random amplified polymorphic DNA (RAPD) and sequence-characterized amplified region (SCAR) marker. Totally, 239 DNA fragments were amplified from six Demodex isolates with 10 random primers in RAPD, of which 165 were polymorphic. Using a single primer, at least five fragments and at most 40 in the six isolates were amplified, whereas within a single isolate, a range of 35-49 fragments were amplified. DNA fingerprints of primers CZ 1-9 revealed intra- and interspecies difference in six Demodex isolates, whereas primer CZ 10 only revealed interspecies difference. The genetic distance and dendrogram showed the intraspecific genetic distances were closer than the interspecific genetic distances. The interspecific genetic distances of Demodex folliculorum and Demodex canis (0.7931-0.8140) were shorter than that of Demodex brevis and D. canis (0.8182-0.8987). The RAPD-SCAR marker displayed primer CZ 10 could be applied to identify the three Demodex species. The 479-bp fragment was specific for D. brevis, and the 261-bp fragment was specific for D. canis. The conclusion was that the RAPD-SCAR multi-marker was effective in molecular identification of three Demodex species. The genetic relationship between D. folliculorum and D. canis was nearer than that between D. folliculorum and D. brevis.

  9. Skin mites in mice (Mus musculus): high prevalence of Myobia sp. (Acari, Arachnida) in Robertsonian mice.

    Science.gov (United States)

    Sastre, Natalia; Calvete, Oriol; Martínez-Vargas, Jessica; Medarde, Nuria; Casellas, Joaquim; Altet, Laura; Sánchez, Armand; Francino, Olga; Ventura, Jacint

    2018-05-04

    Myobia sp. and Demodex sp. are two skin mites that infest mice, particularly immunodeficient or transgenic lab mice. In the present study, wild house mice from five localities from the Barcelona Roberstonian system were analysed in order to detect skin mites and compare their prevalence between standard (2n = 40) and Robertsonian mice (2n > 40). We found and identified skin mites through real-time qPCR by comparing sequences from the mitochondrial 16S rRNA and the nuclear 18S rRNA genes since no sequences are available so far using the mitochondrial gene. Fourteen positive samples were identified as Myobia musculi except for a deletion of 296 bp out to 465 bp sequenced, and one sample was identified as Demodex canis. Sampling one body site, the mite prevalence in standard and Robertsonian mice was 0 and 26%, respectively. The malfunction of the immune system elicits an overgrowth of skin mites and consequently leads to diseases such as canine demodicosis in dogs or rosacea in humans. In immunosuppressed mice, the probability of developing demodicosis is higher than in healthy mice. Since six murine toll-like receptors (TLRs) are located in four chromosomes affected by Robertsonian fusions, we cannot dismiss that differences in mite prevalence could be the consequence of the interruption of TLR function. Although ecological and/or morphological factors cannot be disregarded to explain differences in mite prevalence, the detection of translocation breakpoints in TLR genes or the analysis of TLR gene expression are needed to elucidate how Robertsonian fusions affect the immune system in mice.

  10. Morphometric Study on Male Specimens of Hyalomma Anatolicum (Acari: Ixodidae in West of Iran

    Directory of Open Access Journals (Sweden)

    M Abdigoudarzi

    2011-12-01

    Full Text Available Background: Hyalomma anatolicum is the well-known hard tick, which is one of the most important livestock and hu­man pathogens vector, wide range in host and distributed in all over the Hyalomma geographic fauna as well as in Iran. Taxonomy of the Hyalomma ssp. is debatable whereas their identification is a problematic work. The reasons for this claim is time consuming Delpy’s researches in Iran also Schulze School, Feldman-Muhsam and the Russian tick workers. We would like to understand morphometric variation in the field collected H. anatolicum in Iran also validat­ing some morphologic quantitative and qualitative characters.Methods: A total 247 field-collected tick specimens from different geographical regions in west of Iran includes Khuzestan and Lorestan Provinces were studied. The morphologic characters of the ticks were measured by the cali­brated stereomicroscope ‎armed scaled lens. The measurements were analyzed using SPSS ‎for windows, version 16 on an IBM PC, ‎so varied shapes of species in different geographic ‎regions were drawn by the ‎aid of a drawing tube con­nected to a light stereomicroscope.‎Results: One way ANOVA test revealed significant differences among the quantitative parameters in five zones (P<‎‎ 0.‎‎00‎‎1‎ also each zone to other zone by Post Hoc Tests e.g. LSD. No significant differences in the lateral grooves length/conscutum length ratio parameter were found.Conclusion: Morphometric variation in Hyalomma spp is poorly studied. The variation in range and quantity of the mor­phometric parameters of H.anatolicum ‎underlies that the correct recognition and key construction for Hyalomma spe­cies dependes ‎on a complement morphometric study on the other species.

  11. Attraction of Phytoseiulus persimilis (Acari: Phytoseiidae) towards volatiles from various Tetranychus urticae-infested plant species.

    Science.gov (United States)

    van den Boom, C E M; van Beek, T A; Dicke, M

    2002-12-01

    Plants infested with the spider mite Tetranychus urticae Koch, may indirectly defend themselves by releasing volatiles that attract the predatory mite Phytoseiulus persimilis Athias-Henriot. Several plants from different plant families that varied in the level of spider mite acceptance were tested in an olfactometer. The predatory mites were significantly attracted to the spider mite-infested leaves of all test plant species. No differences in attractiveness of the infested plant leaves were found for predatory mites reared on spider mites on the different test plants or on lima bean. Thus, experience with the spider mite-induced plant volatiles did not affect the predatory mites. Jasmonic acid was applied to ginkgo leaves to induce a mimic of a spider mite-induced volatile blend, because the spider mites did not survive when incubated on ginkgo. The volatile blend induced in ginkgo by jasmonic acid was slightly attractive to predatory mites. Plants with a high degree of direct defence were thought to invest less in indirect defence than plants with a low degree of direct defence. However, plants that had a strong direct defence such as ginkgo and sweet pepper, did emit induced volatiles that attracted the predatory mite. This indicates that a combination of direct and indirect defence is to some extent compatible in plant species.

  12. Side-effects of three pesticides on the predatory mite, Phytoseiulus persimilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Kavousi, A; Talebi, K

    2003-01-01

    Side-effects of three commonly used pesticides in Iran were evaluated on an introduced strain of the predatory mite. Phytoseiulus persimilis Athias-Henriot, reared for about 10 years without exposure to any pesticides. Application of pesticides was carried out either to detached bean leaves using a Potter tower at 1 mg wet deposit per cm2 or by a hand sprayer on bean plants until run off. According to an EPPO decision making scheme, pirimiphos-methyl was found to be harmful (E=90.8%) and heptenophos harmless (E=-3.7%) to the predatory mite in the residual initial toxicity tests. For determination of the hazard class of malathion a field test was found to be necessary (E=59.8%). Categories of 1, 2, 3 were determined for heptenophos, malathion and primiphos-methyl, respectively, using IOBC classification. Despite being harmful, it is possible to use pirimiphos-methyl 10 days before release of P. persimilis. Investigation of the contribution of both lethal and sub-lethal effects to total impact indicated the dominance of lethality in the case of pirimiphos-methyl, while malathion acted by both mechanisms. Heptenophos did not have negative effects on fecundity of P. persimilis but rather caused a higher rate of fecundity in comparison with the control. The mortality found in the heptenophos test was not significantly different from the control.

  13. Disease prevalence and transmission of Microsporidium phytoseiuli infecting the predatory mite, Phytoseiulus persimilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Bjørnson, S; Keddie, B A

    2001-02-01

    Isolated colonies of the predatory mite, Phytoseiulus persimilis, were used to gain information regarding prevalence and transmission of Microsporidium phytoseiuli. Two colonies of P. persimilis were reared on spider mite (Tetranychus urticae)-infested bean plants in isolated cages. Disease prevalence of predators from Colony 1 remained relatively low (between 0 and 15%) over 57 weeks of observation whereas disease prevalence of predators from Colony 2 increased over 3 months (from 12 to 100%). Disease prevalence among predators from Colony 1 had increased to 100% 2 months after weekly sampling had ceased for this colony and periodic sampling confirmed that disease prevalence among individuals of both colonies remained at 100%. Microsporidian spores were not detected in randomly chosen samples of T. urticae prey mites that were removed and examined biweekly during this period. Although numerous microsporidian spores were observed in smear preparations of fecal pellets examined by light microscopy, spores were not observed on leaf surfaces or predator feces when examined by SEM. The latter appeared as intact aggregates composed of numerous dumbbell-shaped crystals and it is unlikely that spores are liberated from intact fecal pellets onto leaf surfaces. Vertical transmission of M. phytoseiuli was 100%; horizontal transmission was low (14.3%) and occurred only when immature P. persimilis were permitted to develop in contact with infected immature and adult predators. The mean number of eggs produced per mated pair was highest when uninfected females were mated with uninfected males (63.2 eggs per mated pair). Although mean egg production decreased when one or both parents were infected, not all differences were significant. Male predatory mites did not contribute to infection of their progeny. Results suggest that routine examination of P. persimilis for microsporidian spores is essential for the management of M. phytoseiuli within P. persimilis colonies. Low disease prevalence and lack of obvious disease signs or symptoms, as in the case of M. phytoseiuli, increase the probability that these pathogens will escape notice unless individuals are routinely examined for pathogens. Copyright 2001 Academic Press.

  14. The effect of temperature on the functional response of Phytoseiulus persimilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Skirvin, David J; Fenlon, John S

    2003-01-01

    Environmental variables, such as temperature, are important in determining the efficiency of biological control in ornamental crops. This paper examines the effect of temperature on the functional response of adult female Phytoseiulus persimilis to eggs of the spider mite, Tetranychus urticae. The functional response was determined using a new functional response assay technique with plant stems as an arena, rather than leaf discs. The use of plant stems allows the influence that plant structure has on predation to be incorporated into the assay. Control assays were also used (without predators) to estimate natural losses of prey. The data were analysed using a binomial model, with the use of Abbot's formula to correct for the losses in the controls. A combined equation to describe the effect of temperature and prey density on the predation rate of Phytoseiulus persimilis was derived. The results showed that more prey are eaten as the temperature increases from 15 degrees C to 25 degrees C, but the number of prey eaten then declines at 30 degrees C, although not to the levels seen at 20 degrees C. The implication of these results for biological control in ornamental crops, where the temperature can often exceed 30 degrees C, is discussed.

  15. Morphological and molecular diagnostics of Phytoseiulus persimilis and Phytoseiulus macropilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Okassa, Mireille; Tixier, Marie-Stéphane; Kreiter, Serge

    2010-11-01

    This study focuses on the diagnostics of two natural enemy species, belonging to the genus Phytoseiulus in the family Phytoseiidae (sub-family Amblyseiinae): P. macropilis and P. persimilis. These two species are of primary importance in biological control all over the world. However, they are morphologically very similar and specific diagnostics is difficult. This study utilizes mitochondrial molecular markers (12S rRNA and Cytb mtDNA) to differentiate these two species. Morphological analyses showed significant differences between P. persimilis and P. macropilis for 17 morphological characters of the 32 considered. However, despite these significant differences, the ranges of all characters overlap. Only the serration of the macroseta on the basitarsus (StIV) allows the differentiation between P. persimilis and P. macropilis. Despite these small morphological differences, molecular results, for both mitochondrial DNA fragments considered (rRNA and Cytb mtDNA), showed a clear delineation between the specimens of P. macropilis and P. persimilis. This study emphasizes (i) that only one morphological character (serration of the seta StIV) clearly separates these two species, and (ii) the usefulness of an automatical molecular and simple diagnostic tool for accurate differentiation of the two species and ensure the morphological diagnostics. Further studies are proposed, including more DNA sequences especially for P. macropilis.

  16. Birefringent Crystals and Abdominal Discoloration in the Predatory Mite Phytoseiulus persimilis (Acari: Phytoseiidae)

    Science.gov (United States)

    Bjørnson; Steiner; Keddie

    1997-03-01

    In response to grower complaints of poor performance of Phytoseiulus persimilis, mites from 14 commercial insectaries and research colonies were examined for pathogens. Some were found to have abdominal discolorations, manifested initially as two white stripes along the dorsal sides of the body within the Malpighian tubules. Advanced signs appeared as a large, centrally located, white spot or U-shaped discoloration in the distal opisthosoma within the rectum/anal atrium. White material often accumulated and hardened within the anus and formed a rectal plug that inhibited further excretion. Most affected mites were lethargic. Adults and immatures with abdominal discoloration contained numerous densely packed, birefringent, dumbbell-shaped entities. Though occasionally observed in the colon, they occurred most frequently within the Malpighian tubules and/or rectum and anal atrium. Dumbbells measured 2-4 &mgr;m long and contained prominent concentric rings. When observed by transmission electron microscopy, the entities lacked cellular organelles. Asymptomatic mites contained few or no such entities. Dumbbell-shaped inclusions were observed in P. persimilis from all sources examined. High levels of potassium, low levels of phosphorous and sulfur, and traces of chlorine were detected by energy-dispersive X-ray analysis. Guanine and uric acid, known nitrogenous wastes of arachnids, do not contain these elements. The chemical composition and structure indicate that the dumbbells are crystals. Both asymptomatic mites and those specimens exhibiting abdominal discoloration were examined for pathogens using light and transmission electron microscopy. Microsporidia, virus-like particles, and a rickettsia (genus Wolbachia) were observed in some mites but showed no correlation with white abdominal discoloration or associated crystal formation. Neither were pathogens always detected in symptomatic mites. Although birefringent crystals may be naturally occurring excretory products, the cause of white abdominal signs associated with crystal accumulation in P. persimilis is unknown. These signs indicate overall poor health.

  17. Abundance and distribution of Microdispus lambi (Acari: Microdispidae) in Spanish mushroom crops.

    Science.gov (United States)

    Navarro, María-Jesús; Gea, Francisco-José; Escudero-Colomar, L Adriana

    2010-04-01

    The myceliophagous mite Microdispus lambi has become a veritable plague since 1996, when it was first observed in Spanish mushroom crops, and is now causing substantial economic losses, particulary in spring and summer. This study looks at seasonal variation of the pest, its distribution on commercial farms and the population development during the crop cycle of the common white mushroom, Agaricus bisporus. Over a period of 18 months, 24 consecutive mushroom crop cycles were monitored and a total of 24 spawn and 960 substrate samples were analysed. We found that it is usually the substrates in the growing rooms that are infested, most commonly the compost. In many cases, the pest can be detected when the first 'flush'-i.e., mushroom growth surge, with weekly periodicity-is harvested, although damage does not become evident until the third flush. Mites were detected at the back of the mushroom growing room and, to a lesser extent, near the access door.

  18. The Bat Tick Carios Azteci (Acari: Argasidae) From Belize, With An Endosymbiotic Coxiellaceae

    Science.gov (United States)

    2016-12-01

    veterinary parasitologists. Similar recent records for bat ticks from Nicaragua (Venzal et al. 2015) also suggest the species is more widespread. Our...agent of either bats or humans. Ectoparasites from cave habitats are of ecological and veterinary interest as they are often poorly studied beyond... Microbiology & Infectious Diseases 31: 365–369. http://dx.doi.org/10.1007/s10096-011-1318-7 Taylor, S.J., Heads, S.W., Jacoby, J., Hoese, G.B., Krejca

  19. A new species of Andocaeculus (Acari, Caeculidae) from the Pampa biome, southern Brazil

    OpenAIRE

    Ana Paula Ott; Ricardo Ott

    2014-01-01

    A new caeculid species Andocaeculus caioi sp. nov. is described from Pampa biome in south Brazil. The species of this family are usually large and strong sclerotized mites with robust and spinulose legs I and II. Until now records of species for South America were known only from Chile and Argentina.

  20. Influence of gamma radiation for controlling Brevipalpus phoenicis (Acari: Tenuipalpidae) (Geijskes, 1939) in oxygen atmosphere

    Energy Technology Data Exchange (ETDEWEB)

    Machi, Andre R., E-mail: rica_machi@hotmail.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN- SP), Sao Paulo, SP (Brazil); Arthur, Valter, E-mail: arthur@cena.usp.br [Centro de Energia na Agricultura (CENA/USP), Piracicaba, SP (Brazil)

    2013-07-01

    Brevipalpus phoenicis mite are controlled across of solutions acaricides, which are chemicals and leave residues in addition there is the difficulty of an effective pulverization due to the small size of the mite, the objective of this study was to evaluate of the influence of oxygen combined with gamma radiation on B.phoenicis as alternative control. Were used 70 mites per arena in 9 reps on 3 treatments at doses of 0 (control), 200 and 300 Gy. For irradiation, the leaves containing the mites, were cut and placed on bottles with bladder tied with ribbons and strings, before was put pure oxygen and the bottle was then sealed, these were taken to a gamma irradiator of Cobalt 60-type Gammacell 220, under a dose rate of 0.381 kGy/hour located in the CENA/USP. Was evaluated daily (eggs, nymphs and adults) of the mites observed viability, fertility and mortality across of the analysis of variance design with completely randomized design using the Statistical Analysis System (SAS) version 9.2® and by the Tukey test, the verification of means. After 22 days of irradiation the hatchability in 200 Gy dose was 41% after 3 days and 57% in control dose, this differed statistically of the other doses, where the nymphs arrived to the adult stage, which did not occurred in the 200 Gy dose and higher due to mutations, generated by the gamma radiation. In 300 Gy not was observed the presence of nymphs and eggs, being the sterilizing dose for all stages of the B.phoenicis. (author)

  1. Canis familiaris, UN NUEVO HOSPEDERO DE Ornithodoros (A. puertoricensis FOX, 1947 (ACARI: IXODIDA EN COLOMBIA

    Directory of Open Access Journals (Sweden)

    LUIS E. PATERNINA

    2009-01-01

    Full Text Available RESUMEN Las garrapatas revisten gran importancia en el campo biomédico por sus hábitos hematófagos y asociación con la transmisión de agentes patógenos a humanos y animales. El objetivo de esta investigación fue establecer las especies de garrapatas que parasitan perros en tres poblaciones del área rural del Caribe colombiano. Durante los meses de agosto y diciembre del año 2006 se realizó búsqueda activa de garrapatas sobre caninos domésticos de las localidades de El Campín, Sabanas del Potrero y Escobar Arriba, departamento de Sucre. Las garrapatas recolectadas fueron almacenadas en viales con etanol al 70% e identificadas empleando claves morfológicas de referencia para cada familia. Para la determinación de especie en la familia Argasidae se realizaron estimaciones morfométricas de estructuras externas. Se recolectaron 420 garrapatas a partir de 50 caninos infestados, de un total de 134 perros examinados, que corresponde a una tasa de infestación del 37,3%. Las garrapatas fueron identificadas como Rhipicephalus sanguineus, Rhipicephalus (Boophilus microplus y Amblyomma ovale pertenecientes a la familia Ixodidae, y Ornithodoros (Alectorobius puertoricensis de la familia Argasidae. La especie predominante fue R. sanguineus (92,1% en los estados de larva, ninfa y adulto, seguida por larvas de O. puertoricensis, que fueron halladas en menor número sobre caninos de las tres localidades. Se registra, por primera vez en América, el parasitismo de O. puertoricensis sobre caninos domésticos y se confirma su presencia en Colombia. Palabras clave: garrapatas, perros, Ornithodoros puertoricensis, Ixodida, Colombia. ABSTRACT Ticks are very important from the biomedical point of view, by their hematophagic activity and their role in the transmission of pathogenic microorganisms to man and animals. The main goal of this work was to establish the tick species parasiting dogs in three rural localities of the Colombian Caribbean. From August to December 2006, an active search of ticks on dogs was carried out in the localities of El Campín, Sabanas del Potrero and Escobar Arriba, department of Sucre. The collected ticks were preserved into eppendorf tubes with 70% ethanol, and identified using standard morphological keys for each family. Argasid species were determined by measuring external morphological characters. Of 134 examined dogs in the three localities, 50 were found infested by ticks, representing a infestation rate of 37,3%. A total of 420 ticks were collected from dogs and identified as Rhipicephalus sanguineus, Rhipicephalus (Boophilus microplus, and Amblyomma ovale of the Ixodidae family, and Ornithodoros puertoricensis of the Argasidae family. R. sanguineus was the predominant species (92,1% in the stages of larva, nymph and adult, following by O. puertoricensis larvae recorded in low numbers in the three regions sampled. The tick O. puertoricensis is recorded for the first time as ectoparasite of domestic dogs in America. Additionally, the presence of this tick species is confirmed in Colombia. Key words: Ticks, dogs, Ornithodoros puertoricensis, Ixodida, Colombia

  2. New records and some interesting findings of oribatid mites (Acari: Oribatida from Georgia

    Directory of Open Access Journals (Sweden)

    M. Murvanidze

    2017-06-01

    Full Text Available Six species of oribatid mites are found first time for Georgian fauna: Eobrachychthonius latior (Berlese, 1910, Graptoppia paraanalis Subias & Rodrigues, 1985, Mongaillardia grandjeani Calugar & Vasiliu 1984, Tritegeus bisulcatus Grandjean 1953, Podoribates longipes (Berlese, 1887, Chamobates birulai Kuiczinsky, 1092 and Oribatula (Zygoribatula skrjabini (Bulanova-Zachvatkina 1967. M. grandjeani is new finding for Caucasus area. New locations for some rare oribatid species are also registered. Remarks on the replacement of genus Berndamerus to family Ctenobelbidae Grandjean, 1965 are given.

  3. Introduction of Astigmatina and Oribatida Mites (Acari: Sarcoptiformes associated with Stored Food Products in Mashhad county

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    Zohre Khaleghabadian

    2016-09-01

    Full Text Available Introduction: The occurrence and activity of mites in stored foods cause a variety of qualitative and quantitative damages, including reduction in the germinating power of the grains, bronchial asthma and allergic skin reactions. Among the mites associated with stored products, species of order Sarcoptiformes especially some species of Astigmatina including the genera Acarus, Suidasia and Tyrophagousare have economic importance and a worldwide distribution. The members of family Histiosomatidae also are cosmopolitan and often found in moist organic materials. In Iran, there have been a considerable number of studies on astigmatic mites. Kamali et al. have provided a list of stored product mites. fthe work of Bahrami et al. on species diversity of Acaroidea in Tehran, Ardeshir on population of stored grains in different seasons in Iran;Sayedi et al. on mites associated with stored rice in Guilan can be mentioned. Reviewing the literature revealed that in the only study that has been carried out in the study area, only 6 species of storage mites have previously been reported from Khorasan-e- +Razavi province. Literature review showed that although Oribatida consists of 9000 known species in the world, the number of recorded species from Iran is not considerable. Iranian studies on oribatid mites have limited to a few specific areas and recent years. By considering the limitation of knowledge on species diversity of stored product mites in the study area, the present study aimed to improve our knowledge on the mite associated with the stored food products in Mashhad city and the vicinity. Material and Methods: During years 2011−2012 a variety of food storages in Mashhad region were visited and sampled. These stored food products included wheat, barley, wheat bran, rice, flour mill, factories of pasta and bakeries, stockpiles of livestock’s food, and potato and onion storages in different parts of Mashhad. Mite specimens were extracted from infested materials by using Berlese-Tullgreen funnel. The specimens were cleared using either pure Lactic acid or Nesbitt’s mixture and then mounted in Hoyer's medium as microscopic slides. Using relevant references, mites were identified and then sent to the third and fourth authors for confirmation. Type specimens are held in the Acarological laboratory, Department of Plant Protection, Ferdowsi University of Mashhad, Iran. Results and Discussion: Among the identified species, Histiostoma sapromyzarum (Dufour has reported from Iran for the first time. New records for the study area are indicated by an asterisk. The list of identifying species is as below: I- Astigmatina- 1- Family Acaridae: Aleuroglyphus ovatus (Troupeau*, Caloglyphus berlesie Michael, Acarus siro Linnaeus, Rhizoglyphus echinopus (Fumouze & Robin*, R. robini Clapared, Tyrophagus longior Gervais*, T.putrescentiae (Schrank, 2- Family Chortoglyphidae: Chortoglyphus arcuatus (Troupeau*, 3- Family Glycyphagidae: Lepidoglyphus destructor (Schrank*, 4- Histiostomatidae: Histiostoma feroniarum (Dufour*, H. sapromyzarum (Dufour, 5- Suidasiidae: Suidasia nesbitti Hughes*. II- Oribatida- 1- Family Aphelacaridae: Aphelacarus acarinus (Berlese*, 2- Family Cosmochthoniidae: Cosmochthonius sp.*, 3- Family Ctenacaridae: Ctenacarus araneola (Grandjean*, 4- Family Galumnidae: Galumnadis cifera Balogh*, Galumnakarajica Mahunka & Akrami*, 5- Family Haplozetidae: Haplozetes sp.*, 6- Family Mesoplophoridae: Mesoplophora sp.*, 7- Family Oppiidae: Lasiobelba sp.*, 8- Family Oribatulidae: Oribatula (Oribatula sp.*, O. (Zygoribatula sp.*, O.(Zygoribatula connexa Berlese*, O. (Zygoribatula exarata Berlese*, 9- Family Scheloribatidae: Scheloribates fimbriatus Thor*. Conclusion: In this study, 12 species of Astigmatina belonging to 9 genera of 5 families and 13 species of oribatid mites were collected and identified. Among them, one species, namely Histiosomas apromyzarum (Dufour is a new record for the fauna of Iran. Twenty species were reported for the first time from Khorasan-e- Razavi province. Certainly, this list is far from reality. As many parts of the province have not been explored faunistically, with further research in different parts of the province, the recorded mite species would be considerably increased. Not only more faunistic studies are required further research on the biology and ecology of the recorded species is suggested. Also, it should be noted that on the basis of our records, the species T. putrescentiae was found to be the most common mite in the stored food products in Mashhad city and its vicinity.

  4. Hidden in the mangrove forest: the cryptic intertidal mite Carinozetes mangrovi sp. nov. (Acari, Oribatida, Selenoribatidae).

    Science.gov (United States)

    Pfingstl, Tobias; Lienhard, Andrea; Jagersbacher-Baumann, Julia

    2014-08-01

    The small archipelago of Bermuda is a geologically young landmass in the Western Atlantic Ocean and recently turned out to be inhabited by a number of intertidal oribatid mites. One newly described species, Carinozetes bermudensis, showed an unusual vast range of habitats like sandy beaches, rocky substrate and mangroves. In the present study, 13 Bermudian populations of C. bermudensis were analysed to verify species integrity of specimens from different microhabitats. A morphometric analysis of 17 continuous variables as well as a molecular genetic investigation of the mitochondrial cytochrome oxidase subunit I revealed the existence of a new species Carinozetes mangrovi sp. nov., inhabiting exclusively intertidal algae growing on mangrove roots. Although both species are morphologically nearly identical, the configuration of the genus-specific ventral carinae represents a clear diagnostic character. The high genetic divergence of approximately 12 % of the cytochrome oxidase subunit I gene sequence between C. bermudensis and C. mangrovi sp. nov. suggests that these two species diverged before the emergence of the Bermuda islands. Accordingly, both of them are older than the geologically young archipelago of Bermuda.

  5. Arrhenotoky and oedipal mating in the northern fowl mite (Ornithonyssus sylviarum (Acari: Gamasida: Macronyssidae

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    McCulloch John B

    2012-12-01

    Full Text Available Abstract Background The northern fowl mite (NFM; Ornithonyssus sylviarum is a blood-feeding ectoparasite of birds and a major pest of poultry in the United States. Mite populations spread rapidly in commercial flocks, reach peak burdens of >70,000 mites per bird and have developed resistance to many pesticides. Despite decades as a pest in the United States, the reproductive biology of NFM remains unclear. Based on karyotypes, the NFM has haplodiploid sex determination, which suggests unmated females could produce male offspring (arrhenotoky. Thus, unmated females could disseminate to a new host and initiate an infestation by producing and mating with sons (oedipal mating. Methods We used small capsules to isolate and recover NFM on host chickens. Mites in capsules could blood feed, develop and reproduce, but could not contact other mites. Individual larvae were matured in isolation to produce known, unmated females. We evaluated reproduction of (I previously mated females (i in isolation, or (ii paired with a male, and (II unmated (virgin females in isolation. In each treatment we recorded the number and sexes of offspring produced over time. Results Mated NFM produced female and male offspring in isolation, or when paired with a male. When paired with a male, females produced a female-biased sex ratio of the offspring (F:M ratio ~5:1. Unmated, female NFM produced exclusively male offspring when in isolation. When paired with their sons that had developed to maturity, the "virgin" females were able to mate and subsequently produce female offspring. Conclusions This study found that females with immediate access to sperm produced mostly female offspring. Virgin female NFM initially produced only male offspring and subsequently used oedipal mating to produce female offspring. Using this reproductive system NFM could successfully colonize new hosts as immature, or unmated females. The strong female-biased sex ratio of NFM populations suggests a large proportion of the parasite population is capable of disseminating to new hosts, which is essential for an obligate parasite to persist.

  6. New taxa and new records of oribatid mites of the family Galumnidae (Acari: Oribatida) from Ecuador

    Czech Academy of Sciences Publication Activity Database

    Ermilov, S.G.; Starý, Josef; Sandmann, D.; Marian, F.; Maraun, M.

    2013-01-01

    Roč. 3700, č. 2 (2013), s. 259-270 ISSN 1175-5326 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Oribatida * Galumnidae * new species * Ecuador Subject RIV: EG - Zoology Impact factor: 1.060, year: 2013

  7. Survey of ticks (Acari: Ixodidae) and tick-borne pathogens in North Dakota.

    Science.gov (United States)

    Russart, Nathan M; Dougherty, Michael W; Vaughan, Jefferson A

    2014-09-01

    Ticks were sampled at nine locations throughout North Dakota during early summer of 2010, using flagging techniques and small mammals trapping. In total, 1,762 ticks were collected from eight of the nine locations. The dominant species were Dermacentor variabilis (Say) (82%), found throughout the state, and Ixodes scapularis Say (17%), found in northeastern counties. A few nymphal and adult I. scapularis tested positive for Borrelia burgdorferi (3%) and Anaplasma phagocytophilum (8%). This is the first report of I. scapularis and associated pathogens occurring in North Dakota and provides evidence for continued westward expansion of this important vector tick species in the United States.

  8. First record of Ophionyssus natricis (Gervais (Acari: Macronyssidae on Python reticulatus (Schneider (Pythonidae in Brazil

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    Ademar da Silva

    2018-04-01

    Resumo. Ophionyssus natricis (Gervais frequentemente parasita cobras mantidas sob cuidados humanos. Este ácaro é conhecido por transmitir mecanicamente a bactéria Proteus hydrophilus, que pode causar sepse hemorrágica em cobras. Este é o primeiro registro O. natricis em Python reticulatus (Schneider coletados em cativeiro, no Brasil. O estudo foi realizado com o auxílio de microscopia óptica e observações de MEV, gerando desenhos e micrografias, o que permitiu observar importantes características de identificação da fêmea de O. natricis: superfície dorsal com dois escudos, consistindo em propodossoma e pigidial, já a superfície ventral possui apenas o escudo esternal com forma trapezoidal, com dois pares de cerdas e poros na borda. A identificação das fêmeas adultas de O. natricis coletados em P. reticulatus mantidas sob cuidados humanos no Brasil, fornece informações anatômicas adicionais para ajudar na identificação da espécie fornecendo mais informações necessárias na compreensão da morfologia dos ácaros das cobras.

  9. Babesia microti (Piroplasmida: Babesiidae) in nymphal Ixodes ricinus (Acari: Ixodidae) in the Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Rudolf, Ivo; Golovchenko, Maryna; Šikutová, Silvie; Rudenko, Natalia; Grubhoffer, Libor; Hubálek, Zdeněk

    2005-01-01

    Roč. 52, č. 3 (2005), s. 274-276 ISSN 0015-5683 R&D Projects: GA ČR(CZ) GA206/03/0726 Institutional research plan: CEZ:AV0Z60930519; CEZ:AV0Z60220518 Keywords : Babesia microti * Ixodes ricinus Subject RIV: EE - Microbiology, Virology Impact factor: 1.138, year: 2005 http://folia.paru.cas.cz/pdfs/showpdf.php?pdf=20766

  10. Phytoseiid mites from tropical fruit trees in Bahia State, Brazil (Acari, Phytoseiidae).

    Science.gov (United States)

    de Souza, Izabel Vieira; Sá Argolo, Poliane; Júnior, Manoel Guedes Correa Gondim; de Moraes, Gilberto José; Bittencourt, Maria Aparecida Leão; Oliveira, Anibal Ramadan

    2015-01-01

    The cultivation of tropical fruit trees has grown considerably in the state of Bahia, northeastern Brazil. Some of these have been severely attacked by phytophagous mites, which are usually controlled by the use of chemical pesticides. However, there is today a growing interest for the adoption of less aggressive measures of pest control, as for example the use of predatory mites. Most of the plant-inhabiting predatory mites belong to the family Phytoseiidae. The objective of this paper is to report the phytoseiid species found in an intensive survey conducted on cultivated tropical fruit trees in fifteen localities of the southern coast of Bahia. Measurements of relevant morphological characters are provided for each species, to complement the understanding of the morphological variation of these species. Twenty-nine species of sixteen genera were identified. A key was elaborated to assist in the separation of these species. Fifteen species are reported for the first time in the state, raising to sixty-six the number of species of this family now known from Bahia. Seventy-two percent of the species collected belong to Amblyseiinae, followed by Typhlodrominae (21%) and Phytoseiinae (7%). The most diverse genus was Amblyseius. Amblyseius operculatus De Leon was the most frequent and abundant species. Studies should be conducted to evaluate the possible role of the most common predators as control agents of the phytophagous mites co-occurring with them.

  11. Three new species of the family Phthiracaridae (Acari, Oribatida) from Bolivia

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2015-01-01

    Roč. 3918, č. 1 (2015), s. 128-140 ISSN 1175-5326 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : ptyctimous mites * new species * taxonomy * morphology * Bolivia Subject RIV: EG - Zoology Impact factor: 0.994, year: 2015

  12. New species and records of cunaxid mites (Acari: Cunaxidae) from soil in Southern Brazil.

    Science.gov (United States)

    Rocha, Matheus Dos Santos; Rodrigues, Everton Nei Lopes; Ferla, Noeli Juarez

    2015-07-02

    Neocunaxoides promatae sp. nov., Bonzia flechtmanni sp. nov. and Dactyloscirus multiscutus sp. nov. are described from soil and leaf litter in Atlantic rainforest and Atlantic Araucaria forest in natural environments in São Francisco de Paula municipality, Rio Grande do Sul State, Brazil. In addition, the species Pulaeus quadrisolenidius Castro & Den Heyer 2009 and Lupaeus lectus Castro & Den Heyer (2009) are registered for the first time in this State.

  13. Feather mites (Acari, Astigmata associated with birds in an Atlantic Forest fragment in Northeastern Brazil

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    HM Silva

    Full Text Available AbstractThe present study reports associations between feather mites (Astigmata and birds in an Atlantic Forest fragment in Rio Grande do Norte state, in Brazil. In the laboratory, mites were collected through visual examination of freshly killed birds. Overall, 172 individuals from 38 bird species were examined, between October 2011 and July 2012. The prevalence of feather mites was 80.8%, corresponding to 139 infested individuals distributed into 30 species and 15 families of hosts. Fifteen feather mite taxa could be identified to the species level, sixteen to the genus level and three to the subfamily level, distributed into the families Analgidae, Proctophyllodidae, Psoroptoididae, Pteronyssidae, Xolalgidae, Trouessartiidae, Falculiferidae and Gabuciniidae. Hitherto unknown associations between feather mites and birds were recorded for eleven taxa identified to the species level, and nine taxa were recorded for the first time in Brazil. The number of new geographic records, as well as the hitherto unknown mite-host associations, supports the high estimates of diversity for feather mites of Brazil and show the need for research to increase knowledge of plumicole mites in the Neotropical region.

  14. New records of ectoparasitic Acari (Arachnida) and Streblidae (Diptera) from bats in Jalisco, Mexico.

    Science.gov (United States)

    Martínez, Maria M Ramírez; Lopez, M Pilar Ibarra; Iñiguez-Dávalos, Luis Ignacio; Yuill, Thomas; Orlova, Maria V; Reeves, Will K

    2016-12-01

    Ectoparasites of bats in the Neotropics are diverse and play numerous ecological roles as vectors of microbial pathogens and endoparasites and as food sources for other cave fauna living both on their hosts and in bat roosts. The ectoparasites of bats in Jalisco State of western Mexico have not been as well described as those of other states with recent checklists that have focused primarily on the Yucatan Peninsula. We captured bats from 2011-2015 on the south coast and Sierra de Amula, Jalisco using mist nets, and we removed ectoparasites by hand. We identified 24 species of streblid bat flies and six ectoparasitic mites from bats caught in mist nets. There were an additional eight possibly undescribed species of Streblidae. Our collections extend the known range of species into Jalisco. © 2016 The Society for Vector Ecology.

  15. A new species of Eutrachytes (Acari: Uropodina: Eutrachytidae) associated with the indian mangrove (Avicennia officinalis)

    Digital Repository Service at National Institute of Oceanography (India)

    Moraza, M.L.; Kontschan, J.; Sahoo, G.; Ansari, Z.A.

    . Prague, Academia and The Hague, SPB Academic Publishing bv. pp. 349-356. Krantz G.W. 1969 — The Mites of Quintana Roo. I. A new species of Eutrachytes from the Yucatan Peninsula, with observations on the classification of the genus — Ann. Entomol. Soc. Am...

  16. Biology of Amblyomma aureolatum (Pallas, 1772 (Acari: Ixodidae on some laboratory hosts in Brazil

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    Daniel Sobreira Rodrigues

    2002-09-01

    Full Text Available The ixodid Amblyomma aureolatum is suspected to play a role in the epidemiology of wild life-cycle hemoparasites, which frequently infect dogs in rural and hunting areas in Brazil. Little is known about its bionomics. The objective of the present study was to evaluate some bionomic aspects of A. aureolatum ticks in Brazil. One engorged female, collected from a dog (Canis familiaris in São Sebastião das Águas Claras, State of Minas Gerais, was used to establish a colony in the laboratory. Subsequently its parasitic stage progeny were fed on domestic dogs and laboratory animals. The free-living stages were incubated at 27ºC ± 2°C and minimum 70% relative humidity in a BOD incubator. The egg incubation period ranged from 31 to 34 days; the parasitic period of larvae ranged from 4 to 6 days and ecdysis to nymphs occurred from day 19 up to day 22. The parasitic period of nymphs ranged from 5 to 8 days and the period of ecdysis to adults from 31 to 33 days. The parasitic period of adults ranged from 11 to 15 days, the pre-oviposition period from 6 to 12 days, and the oviposition period from 9 to 38 days. The total duration of the life cycle ranged from 116 to 168 days.

  17. A checklist of chiggers from Brazil, including new records (Acari: Trombidiformes: Trombiculidae and Leeuwenhoekiidae)

    Science.gov (United States)

    Jacinavicius, Fernando de Castro; Bassini-Silva, Ricardo; Mendoza-Roldan, Jairo Alfonso; Pepato, Almir Rogério; Ochoa, Ronald; Welbourn, Cal; Barros-Battesti, Darci Moraes

    2018-01-01

    Abstract A checklist of the family Trombiculidae and Leeuwenhoekiidae is presented, containing 63 species in 30 genera of chiggers from 80 different hosts and 146 localities in Brazil. The type locality and depository are provided, including new locality and host records for the country. PMID:29670435

  18. Molecular Effects of Irradiation (Cobalt-60 on the Control of Panonychus citri (Acari: Tetranychidae

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    Ke Zhang

    2015-11-01

    Full Text Available The effective dose of irradiation to control pest mites in quarantine has been studied extensively, but the molecular mechanisms underlying the effects of the irradiation on mites are largely unknown. In this study, exposure to 400 Gy of γ rays had significant (p < 0.05 effects on the adult survival, fecundity and egg viability of Panonychus citri. The irradiation caused the degradation of the DNA of P. citri adults and damaged the plasma membrane system of the egg, which led to condensed nucleoli and gathered yolk. Additionally, the transcriptomes and gene expression profiles between irradiated and non-irradiated mites were compared, and three digital gene expression libraries were assembled and analyzed. The differentially expressed genes were putatively involved in apoptosis, cell death and the cell cycle. Finally, the expression profiles of some related genes were studied using quantitative real-time PCR. Our study provides valuable information on the changes in the transcriptome of irradiated P. citri, which will facilitate a better understanding of the molecular mechanisms that cause the sterility induced by irradiation.

  19. An Initial Classification of Neotropical Water Mites (Acari: Hydrachnidia Based on Habitat Preferences

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    Hugo R. Fernández

    2011-01-01

    Full Text Available Existing classifications of benthic and interstitial freshwater invertebrates are described and discussed. A classification is proposed for southern neotropical (south of latitude S 15 water mites in relation to their life style and habitat preferences. The classification includes planktonic, superficial, benthic, thermal, and subterranean forms. The diversity of the Hydrachnidia family and genera (22 families, 97 genera, and 521 species is then analyzed using the new classification. Ubiquitous stygobites deserve special consideration because they move through ecotone zones and tolerate extreme conditions. Water mite communities from a north-western Argentinean stream were first described using a surber net and consequently considered as benthic. Nineteen Hydrachnidia species (from benthic to stygobite were collected and classified. The vertical distribution observed during the year confirmed the permanent presence of benthic Hydrachnidia, even during the first flood, which is of special importance in running waters. The functional classification we propose will facilitate comparison of fauna from different areas that have different faunistic composition but may have similar functional distribution.

  20. Factors Influencing Mesostigmata Mites (Acari, Parasitiformes In The Alkaline Fen Habitats

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    Salmane Ineta

    2015-04-01

    Full Text Available Mesostigmata mite fauna was investigated in soil of one alkaline fen at Apšuciems in the Maritime Lowland in Latvia in 2009. Thirty sample plots were selected in the following way: half of them were located in Brown Bog Rush, Schoenus ferrugineus and half — in Saw-Sedge, Cladium mariscus prevailing habitat. Soil samples were collected using a soil borer. Vegetation cover was described in accordance with Braun-Blanquet classes. Mites were extracted using modified Berlese funnels. In total, more than 28 species were recorded, of them Prozercon kochi and Pergamasus vagabundus were dominant species. Mean density of Mesostigmata mites ranged from 520 to 2140 ind./m2. Mite abundance and distribution between habitats depended on vegetation cover of the vascular plants, while moss cover and soil pH had no significant influence.

  1. Laboratory Study on Biological Control of Ticks (Acari: Ixodidae by Entomopathogenic Indigenous Fungi (Beauveria bassiana

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    M Abdigoudarzi

    2009-12-01

    Full Text Available Background: Chemical control method using different acaricides as spray, dipping solution or pour-on is routinely used for controlling ticks. Biological control agents are favorable due to their safety for animals and environment. Entomopathogenic fungi such as Beauveria bassiana are well known for controlling ticks. In this study, two Iranian indigenous strains of B. bassiana (B. bassiana 5197 and B. bassiana Evin were selected and grown on specific me­dia. The pathogenic effects of these strains were evaluated on adult stages of two Iranian Ixodidae members (H. anatolicum anatolicum Koch 1844, and H. marginatum Koch 1844 by dipping method.Methods: Two Iranian strains of Beauveria bassiana (Beauveria bassiana 5197 and Beauveria bassiana Evin were selected and were grown successfully on specific media. The pathogenic effects of these strains were evaluated on adult stages of Iranian Ixodidae members such as, Hyalomma anatolicum anatolicum and H. marginatum by dipping method (these ticks were grown up at laboratory conditions during 2002 up to 2003 and still it is continued .Results: There was no effect of strain 5197 on mortality or fecundity rates for ticks. There was acute phase sign of paralysis in test group after dipping ticks in suspension made from Evin strain of B. bassiana. In addition, the test groups were totally died after four months, but the control groups survived for six months.Conclusion: High concentration of fungal spores is needed for inducing fungal infection. Additional study using different strains and fungi on Iranian ticks is proposed. 

  2. Essential oils and Beauveria bassiana against Dermanyssus gallinae (Acari: Dermanyssidae): Towards new natural acaricides.

    Science.gov (United States)

    Immediato, Davide; Figueredo, Luciana Aguiar; Iatta, Roberta; Camarda, Antonio; de Luna, Rafaela Lira Nogueira; Giangaspero, Annunziata; Brandão-Filho, Sinval Pinto; Otranto, Domenico; Cafarchia, Claudia

    2016-10-15

    Essential oils (EOs) and entomopathogenic fungi such as Beauveria bassiana (Bb) strains have the potential to be used as alternative insecticides and acaricides for controlling ectoparasites as Dermanyssus gallinae. These compounds have some limitations in their use: the acaricidal effect of EOs is rapid, but short-lived, whilst that of Bb is delayed, but long-lived. To evaluate the effect of both compounds combined against D. gallinae, the non-toxic dose of Eucalyptus globulus, Eucalyptus citriodora, Thymus vulgaris and Eugenia caryophyllata essential oils were firstly calculated for "native" strains of Bb. Subsequently, the effects of the combination of selected EOs with Bb against nymph and adult poultry red mites (PRMs) was assessed. EO concentrations ranging from 0.0015 to 8% v/v (i.e., nine double dilutions) were used to evaluate their effect on germination, sporulation and vegetative growth rates of native strains of Bb. A total of 1440 mites (720 nymphs and 720 adults) were divided into three-treated group (TGs) and one control group (CG). In TGs, mites were exposed to Bb in combination with the selected EO (TG1), EO alone (TG2) or Bb (TG3) alone. In the CG, mites were exposed to 0.1% tween 80 plus EO solvent (CG). E. globulus and E. citriodora were toxic for Bb in concentrations higher than 0.2% and 0.003% respectively, whilst E. caryophyllata and T. vulgaris were toxic at all concentrations tested against Bb. Based on the results of the toxicity assays against Bb, E. globulus was chosen to be tested as acaricide resulting non-toxic for Bb at concentration lower than 0.4%. Increased mortality of D. gallinae adults was recorded in TG1 than those in other TGs from 4days post-infection (T+4DPI). A 100% mortality of D. gallinae was recorded in adults at T+9DPI and at T+10DPI in nymphs in TG1 and later than T+11DPI in the other TGs. Used in combination with E. globulus, Bb displayed an earlier acaricidal effect towards both haematophagous D. gallinae stages. The combination of B. bassiana and E. globulus at 0.2% might be used for controlling arthropods of medical and veterinary importance as D. gallinae. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Feather mites (Acari, Astigmata from Azorean passerines (Aves, Passeriformes: lower species richness compared to European mainland

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    Rodrigues Pedro

    2015-01-01

    Full Text Available Ten passerine species were examined on three islands of the Azores (North Atlantic during 2013 and 2014 in order to identify their feather mite assemblages. We recorded 19 feather mite species belonging to four families of the superfamily Analgoidea (Analgidae, Proctophyllodidae, Psoroptoididae and Trouessartiidae. A high prevalence of feather mite species was recorded on the majority of the examined host species. Only three passerine species (Sylvia atricapilla, Regulus regulus and Serinus canaria presented the same full complex of mite species as commonly occurs in the plumage of their closest relatives in continental Europe. Passer domesticus presented the same limited fauna of feather mites living in the plumage as do its co-specifics in continental Europe. Carduelis carduelis bears the same feather mite species as do most of its continental populations in Europe, but it lacks one mite species occurring on this host in Egypt. Turdus merula, Pyrrhula murina and Fringilla coelebs are missing several mite species common to their continental relatives. This diminution could be explained by the founder effect, whereby a limited number of colonizing individuals did not transport the full set of feather mite species, or by the extinction of some mite species after initially having reached the Azores. The only individual of Motacilla cinerea sampled in this study presented a new host record for the mite species Trouessartia jedliczkai.

  4. American Black Bears as Hosts of Blacklegged Ticks (Acari: Ixodidae) in the Northeastern United States.

    Science.gov (United States)

    Zolnik, Christine P; Makkay, Amanda M; Falco, Richard C; Daniels, Thomas J

    2015-09-01

    Ticks and whole blood were collected from American black bears (Ursus americanus Pallas) between October 2011 and October 2012 across four counties in northwestern New Jersey, an area where blacklegged ticks (Ixodes scapularis Say) and their associated tick-borne pathogens are prevalent. Adult American dog ticks (Dermacentor variabilis Say) were the most frequently collected tick species in late spring, whereas adult and nymphal blacklegged ticks were found in both the late spring and fall months. Additionally, for blacklegged ticks, we determined the quality of bloodmeals that females acquired from black bears compared with bloodmeals from white-tailed deer (Odocoileus virginianus Zimmerman), the most important host for the adult stage of this tick species. Measures of fecundity after feeding on each host species were not significantly different, suggesting that the bloodmeal a female blacklegged tick acquires from a black bear is of similar quality to that obtained from a white-tailed deer. These results establish the American black bear as both a host and quality bloodmeal source to I. scapularis. Thus, black bears may help support blacklegged tick populations in areas where they are both present. In addition, samples of black bear blood were tested for DNA presence of three tick-borne pathogens. Anaplasma phagocytophilum Foggie and Babesia microti Franca were found in 9.2 and 32.3% of blood samples, respectively. All blood samples were quantitative polymerase chain reaction-negative for Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt, & Brenner. Although circulating pathogens were found in blood, the status of black bears as reservoirs for these pathogens remains unknown. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  5. CONTRIBUTION TO THE KNOWLEDGE OF THE ARACHNIDS IN THE YUCATAN PENINSULA, MEXICO (EXCLUDING ARANAE AND ACARI

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    Hugo Delfin Gonzalez

    2017-08-01

    Full Text Available The Chelicerata are the second group of arthropods with the highest diversity after insects and they can inhabit almost all types of environments. The most current classification recognizes 11 orders and estimates in the number of species vary from 52,000 to 100,000. We have made an extensive literature review on the diversity of arachnids in the Yucatan Peninsula (YP (excluding spiders and ticks. In Mexico there are 834 known species which represent 6% of the worldwide diversity. In the YP 63 records were found (58 species and 5 genera of arachnids, which represent 6.8% of the Mexican species. According to our research, 28 of the 58 species (48% in the YP were also record in other parts of Mexico, the continent and the world. Undoubtedly, the state of Yucatan is the best represented of the YP. In order to have a better understanding of the diversity of arachnid species is important to promote biological compendiums and sampling programs, which will improve the representation of this group and probably increasing the number of local species.

  6. CONTRIBUTION TO THE KNOWLEDGE OF THE ARACHNIDS IN THE YUCATAN PENINSULA, MEXICO (EXCLUDING ARANAE AND ACARI)

    OpenAIRE

    Hugo Delfin Gonzalez; Virginia Meléndez-Ramírez; Pablo C. Manrique-Saide; Abdiel Martin-Park; Carlos Arisqueta-Chablé

    2017-01-01

    The Chelicerata are the second group of arthropods with the highest diversity after insects and they can inhabit almost all types of environments. The most current classification recognizes 11 orders and estimates in the number of species vary from 52,000 to 100,000. We have made an extensive literature review on the diversity of arachnids in the Yucatan Peninsula (YP) (excluding spiders and ticks). In Mexico there are 834 known species which represent 6% of the worldwide diversity. In the YP...

  7. Factors affecting patterns of Amblyomma triste (Acari: Ixodidae) parasitism in a rodent host.

    Science.gov (United States)

    Colombo, Valeria C; Nava, Santiago; Antoniazzi, Leandro R; Monje, Lucas D; Racca, Andrea L; Guglielmone, Alberto A; Beldomenico, Pablo M

    2015-07-30

    Here we offer a multivariable analysis that explores associations of different factors (i.e., environmental, host parameters, presence of other ectoparasites) with the interaction of Amblyomma triste immature stages and one of its main hosts in Argentina, the rodent Akodon azarae. Monthly and for two years, we captured and sampled rodents at 16 points located at 4 different sites in the Parana River Delta region. The analyses were conducted with Generalized Linear Mixed Models with a negative binomial response (counts of larvae or nymphs). The independent variables assessed were: (a) environmental: trapping year, season, presence of cattle; type of vegetation (natural grassland or implanted forest); rodent abundance; (b) host parameters: body length; sex; body condition; blood cell counts; natural antibody titres; and (c) co-infestation with other ectoparasites: other stage of A. triste; Ixodes loricatus; lice; mites; and fleas. Two-way interaction terms deemed a priori as relevant were also included in the analysis. Larvae were affected by all environmental variables assessed and by the presence of other ectoparasites (lice, fleas and other tick species). Host factors significantly associated with larval count were sex and levels of natural antibodies. Nymphs were associated with season, presence of cattle, body condition, body length and with burdens of I. loricatus. In most cases, the direction and magnitude of the associations were context-dependent (many interaction terms were significant). The findings of greater significance and implications of our study are two. Firstly, as burdens of A. triste larvae and nymphs were greater where cattle were present, and larval tick burdens were higher in implanted forests, silvopastoral practices developing in the region may affect the population dynamics of A. triste, and consequently the eco-epidemiology of Rickettsia parkeri. Secondly, strong associations and numerous interactions with other ectoparasites suggest that co-infestations may be more important for tick dynamics than has so far been appreciated. Copyright © 2015 Elsevier B.V. All rights reserved.

  8. Analyses of hemolymph from Amblyomma cajennense (Acari: ixodidae) using neutron activation analysis (NAA)

    Energy Technology Data Exchange (ETDEWEB)

    Simons, Simone M.; Oliveira, Daniella G.L.; Chudzinski-Tavassi, Ana M., E-mail: daniellaoliveira@butantan.gov.b, E-mail: amchudzinki@butantan.gov.b [Instituto Butantan, Sao Paulo, SP (Brazil); Zamboni, Cibele B., E-mail: czamboni@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    Instrumental neutron activation analysis technique (INAA) was applied to determine the elemental composition of hemolymph from Amblyomma cajennense tick. This biological material came from Butantan Institute (Sao Paulo city, Brazil) and it was investigated using the IEA-R1 nuclear reactor (4MW, pool type) at IPEN/CNEN-SP - Brazil. The concentration values for: Br (0.0032 {+-} 0.0005gL{sup -1}), Ca (0.104 {+-} 0.029gL{sup -1}), Cl (4.41 {+-} 0.25gL{sup -1}), I (76 {+-} 27{mu}gL{sup -1}), K (0.38 {+-} 0.09gL{sup -1}), Mg (0.038 {+-} 0.011gL{sup -1}), Na (4.30 {+-} 0.26gL{sup -1}) and S (1.35 {+-} 0.37gL{sup -1}) were determined for the first time. These data were compared with the concentration values established for Americanum and Anatolicum Excavatum tick species to clarify the ion balance in this biological material (hemolymph). This comparison suggests that Na concentration, majority in these species, has a similar behavior. These data also contribute to the understanding of hemolymph composition complementing its characterization as well as for the understanding of several physiological processes, especially those related to salivary secretion. (author)

  9. Spider mite (Acari: Tetranychidae) mitochondrial COI phylogeny reviewed: host plant relationships, phylogeography, reproductive parasites and barcoding

    NARCIS (Netherlands)

    Ros, V.I.D.; Breeuwer, J.A.J.

    2007-01-01

    The past 15 years have witnessed a number of molecular studies that aimed to resolve issues of species delineation and phylogeny of mites in the family Tetranychidae. The central part of the mitochondrial COI region has frequently been used for investigating intra- and interspecific variation. All

  10. Amblyomma dissimile (Acari: Ixodidae PARÁSITO DE Boa constrictor EN COLOMBIA

    Directory of Open Access Journals (Sweden)

    Ana Torres M

    2009-08-01

    Full Text Available Objetivo. Describir algunas garrapatas encontradas en dos ejemplares de Boa constrictor, llevados al Centro de Atención y Valoración de Fauna Silvestre en Montería, Córdoba, Colombia. Materiales y métodos. Se recolectaron 37 garrapatas provenientes de dos individuos adultos de Boa constrictor, los parásitos fueron conservados en alcohol al 70% y posteriormente identificados mediante diversas claves taxonómicas. Resultados. Todas las garrapatas fueron identificadas como Amblyomma dissimile, de las cuales, 9 fueron hembras, 24 machos y 4 ninfas. Conclusiones. La identificación de ectoparásitos en especies de Boa c. constrictor contribuye a mantener adecuadamente esta especie en cautiverio y provee datos para establecer medidas profilácticas y tratamiento, igualmente, ayuda en el conocimiento de los agentes parasitarios de la fauna silvestre.

  11. Two new species of the genus Pergalumna (Acari, Oribatida, Galumnidae) from Northern Vietnam

    Czech Academy of Sciences Publication Activity Database

    Ermilov, S.G.; Starý, Josef

    2017-01-01

    Roč. 22, č. 4 (2017), s. 494-508 ISSN 1362-1971 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : galumnid mites * new species * morphology * systematics * Tam Dao National Park * Oriental region Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 1.467, year: 2016

  12. Pyrosequencing based assessment of bacterial diversity in Turkish Rhipicephalus annulatus and Dermacentor marginatus ticks (Acari: Ixodidae).

    Science.gov (United States)

    Tekin, Saban; Dowd, Scot E; Davinic, Marko; Bursali, Ahmet; Keskin, Adem

    2017-03-01

    Ticks continue to be a threat to human and animal health in Turkey, as they are considered important vectors of human and animal diseases. The objectives of this investigation are to characterize the microbial communities of two tick species, Rhipicephalus annulatus and Dermacenter marginatus, analyze patterns of co-occurrence among microbial taxa, identify and compare pathogens contributing human diseases, and determine whether avirulent symbionts could exclude human pathogens from tick communities. Furthermore, this study explores a microbiome of the R. annulatus and D. marginatus via the bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) technique to describe their bacterial diversity. Pyrosequencing was performed on adult males and females isolated from humans from two high-risk Turkish provinces, Sivas and Amasya, during tick outbreaks in 2009. A total of 36,253 sequences were utilized for analyses of the 8 tick samples. Several pathogenic genera such as Francisella, Coxiella, Rickettsia, and Shigella were detected in the ticks tested. The most distinguishable difference between the two species of ticks was the lack of known human pathogen Rickettsia in R. annulatus and in samples 9 and 10 of D. marginatus. These samples had higher relative abundance of Flavobacterium sp., Curvibacter sp., Acidovorax sp., and Bacteroidaceae genera mostly representing symbionts which form a large component of normal tick microbiota. The outcome of this study is consistent with the predictions of the community ecological theory that diversity-rich bacteriomes are more resistant to bacterial invasion (and consequent pathogen dissemination) than diversity-deprived ones.

  13. Inhibition of the classical pathway of the complement system by saliva of Amblyomma cajennense (Acari: Ixodidae).

    Science.gov (United States)

    Franco, Paula F; Silva, Naylene C S; Fazito do Vale, Vladimir; Abreu, Jéssica F; Santos, Vânia C; Gontijo, Nelder F; Valenzuela, Jesus G; Pereira, Marcos H; Sant'Anna, Mauricio R V; Gomes, Alessandra P S; Araujo, Ricardo N

    2016-05-01

    Inhibition of the complement system during and after haematophagy is of utmost importance for tick success in feeding and tick development. The role of such inhibition is to minimise damage to the intestinal epithelium as well as avoiding inflammation and opsonisation of salivary molecules at the bite site. Despite its importance, the salivary anti-complement activity has been characterised only in species belonging to the Ixodes ricinus complex which saliva is able to inhibit the alternative and lectin pathways. Little is known about this activity in other species of the Ixodidae family. Thus, the aim of this study was to describe the inhibition of the classical pathway of the complement system by the saliva of Amblyomma cajennense at different stages of the haematophagy. The A. cajennense saliva and salivary gland extract (SGE) were able to inhibit the complement classical pathway through haemolytic assays with higher activity observed when saliva was used. The anti-complement activity is present in the salivary glands of starving females and also in females throughout the whole feeding process, with significant higher activity soon after tick detachment. The SGE activity from both females fed on mice or horses had no significant correlation (p > 0.05) with tick body weight. The pH found in the intestinal lumen of A. cajennense was 8.04 ± 0.08 and haemolytic assays performed at pH 8.0 showed activation of the classical pathway similarly to what occurs at pH 7.4. Consequently, inhibition could be necessary to protect the tick enterocytes. Indeed, the inhibition observed by SGE was higher in pH 8.0 in comparison to pH 7.4 reinforcing the role of saliva in protecting the intestinal cells. Further studies should be carried out in order to identify the inhibitor molecule and characterise its inhibition mechanism. Copyright © 2016 Elsevier Inc. All rights reserved.

  14. A new species of the genus Acarothrix (Acari: Halacaridae) from Brunei Darussalam and India

    Digital Repository Service at National Institute of Oceanography (India)

    Chatterjee, T.; Marshall, D.J.; Guru, B.C.; Ingole B.S.; Pesic V.

    Acarothrix grandocularis sp. nov. is described from specimens collected in Brunei Darussalam and India. The new species is characterized by the presence of dorsal seta 1 on the posterior part of anterior dorsal plate, a triangular posterior margin...

  15. An overview of Suctorian ciliates (Ciliophora, Suctorea) as epibionts of halacarid mites (Acari, Halacaridae)

    Digital Repository Service at National Institute of Oceanography (India)

    Dovgal, I.; Chatterjee, T.; Ingole, B.S.

    Subclass Exogenia Collin Order Metacinetida Jankowski Family Paracinetidae Jankowski Limnoricus ceter Jankowski, 1981 (Fig. 1–3) Diagnosis: Marine commensal, loricate, suctorian ciliate with stalk delimited from posterior margin of lorica. Both lorica...–11) =Thecacineta halacari Schulz, 1933 DOVGAL ET AL.62 · Zootaxa 1810 © 2008 Magnolia Press =Lissacineta allgeni Jankowski, 1981, syn. n. =Thecacineta allgeni (Jankowski, 1981), syn. n. Diagnosis: Marine suctorians with stylotheca. Cell body, slightly...

  16. Infestation of mammals by Ixodes ricinus ticks (Acari: Ixodidae) in south-central Sweden.

    Science.gov (United States)

    Tälleklint, L; Jaenson, T G

    1997-12-01

    Infestation by Ixodes ricinus ticks on rodents, hares and cervids was examined at Bogesund, 10 km north of Stockholm, in south-central Sweden during 1991-1994 and on varying hares (Lepus timidus) at Stora Karlsö and Gotska Sandön in the Baltic Sea during 1992-1993. At Bogesund, there were great differences between two consecutive years in the number of I. ricinus larvae infesting bank voles (Clethrionomys glareolus). The seasonal pattern of infestation by I. ricinus larvae and nymphs on bank voles was unimodal in 1991, with peaks in June-July and bimodal in 1992, with peaks in June and August. Male bank voles, compared to females and older voles, compared to young voles, harboured greater numbers of I. ricinus ticks. Apodemus mice, compared to bank voles, harboured greater numbers of I. ricinus ticks. Ixodes ricinus larvae engorged on Apodemus mice were heavier than larvae engorged on bank voles and resulted in larger nymphs. However, there was no difference in the proportions of viable nymphs resulting from larvae engorged on mice or voles. The ranges in the numbers of I. ricinus ticks infesting individual hosts were 1-451 for rodents, 16-2374 for hares and 428-2072 for roe deer (Capreolus capreolus). These ranges of tick numbers are estimated to represent potential blood losses from individual hosts of approximately 0.2-65% for rodents, 0.2-13% for hares and 0.3-9.0% for roe deer. Within the populations of all host species examined, the distributions of all stages of I. ricinus were clumped, with most host individuals harbouring few ticks and only a few individuals harbouring many ticks. The data suggest that, even though a small proportion of tick hosts may be severely affected, the direct effects of feeding by I. ricinus are unlikely to play an important role on mammal population dynamics.

  17. A new genus and species in the mite family Eupodidae (Acari, Eupodoidea) from Crimea.

    Science.gov (United States)

    Khaustov, Alexander A

    2014-01-01

    A new genus Pseudoeupodes Khaustov, gen. n. and new species Pseudoeupodes porosus sp. n. are described from moss in Crimea. The taxonomy of the Eupodidae and some other families and genera of Eupodoidea is reviewed. The genus Turanopenthalodes Barilo, 1988 is transferred from Penthalodidae to Penthaleidae. The family Cocceupodidae Jesionowska, 2010 and the genus Filieupodes Jesionowska, 2010 are considered as junior synonyms of Eupodidae Koch, 1842 and Cocceupodes Thor, 1934, respectively. A key to genera of the family Eupodidae is provided.

  18. Three new species of the family Phthiracaridae (Acari, Oribatida) from Bolivia.

    Science.gov (United States)

    Niedbała, Wojciech; Starý, Josef

    2015-01-10

    Three new species of the family Phthiracaridae, Austrophthiracarus longisetosus sp. nov., Phthiracarus allocotos sp. nov., Protophthiracarus amboroensis sp. nov. from Bolivia are described and figured. A comparison of morphological similarities with the most closely related species is presented. Additional descriptions and taxonomical notes for three ptyctimous species: Acrotritia peruensis (Hammer, 1961), Acrotritia vestita (Berlese, 1913), and Steganacarus (Rhacaplacarus) sedecimus Niedbała, 2004 are added. A list of twenty six ptyctimous species from Bolivia is presented, ten of these species are new records for the fauna of Bolivia. A key to all species of ptyctimous mites of Bolivia is presented.

  19. A new species of the genus Neogalumna (Acari, Oribatida, Galumnidae from China

    Directory of Open Access Journals (Sweden)

    Liang, Wenqin

    2014-06-01

    Full Text Available A new species of oribatid mites of the family Galumnidae, Neogalumna longiporosa sp. nov., is described from dark loamy soil collected under moss in North Eastern China. It is the first identified member of the genus Neogalumna recorded for China. An identification key to the known species of Neogalumna is also given.

  20. Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae), the Cayenne tick: phylogeography and evidence for allopatric speciation.

    Science.gov (United States)

    Beati, Lorenza; Nava, Santiago; Burkman, Erica J; Barros-Battesti, Darci M; Labruna, Marcelo B; Guglielmone, Alberto A; Cáceres, Abraham G; Guzmán-Cornejo, Carmen M; León, Renato; Durden, Lance A; Faccini, João L H

    2013-12-09

    Amblyomma cajennense F. is one of the best known and studied ticks in the New World because of its very wide distribution, its economical importance as pest of domestic ungulates, and its association with a variety of animal and human pathogens. Recent observations, however, have challenged the taxonomic status of this tick and indicated that intraspecific cryptic speciation might be occurring. In the present study, we investigate the evolutionary and demographic history of this tick and examine its genetic structure based on the analyses of three mitochondrial (12SrDNA, d-loop, and COII) and one nuclear (ITS2) genes. Because A. cajennense is characterized by a typical trans-Amazonian distribution, lineage divergence dating is also performed to establish whether genetic diversity can be linked to dated vicariant events which shaped the topology of the Neotropics. Total evidence analyses of the concatenated mtDNA and nuclear + mtDNA datasets resulted in well-resolved and fully congruent reconstructions of the relationships within A. cajennense. The phylogenetic analyses consistently found A. cajennense to be monophyletic and to be separated into six genetic units defined by mutually exclusive haplotype compositions and habitat associations. Also, genetic divergence values showed that these lineages are as distinct from each other as recognized separate species of the same genus. The six clades are deeply split and node dating indicates that they started diverging in the middle-late Miocene. Behavioral differences and the results of laboratory cross-breeding experiments had already indicated that A. cajennense might be a complex of distinct taxonomic units. The combined and congruent mitochondrial and nuclear genetic evidence from this study reveals that A. cajennense is an assembly of six distinct species which have evolved separately from each other since at least 13.2 million years ago (Mya) in the earliest and 3.3 Mya in the latest lineages. The temporal and spatial diversification modes of the six lineages overlap the phylogeographical history of other organisms with similar extant trans-Amazonian distributions and are consistent with the present prevailing hypothesis that Neotropical diversity often finds its origins in the Miocene, after the Andean uplift changed the topology and consequently the climate and ecology of the Neotropics.

  1. New species of .i.Notophthiracarus./i. (Acari: Oribatida: Phthiracaroidea) from Madagascar

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2014-01-01

    Roč. 64, č. 1 (2014), s. 79-86 ISSN 0003-4541 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : oribatid * ptyctimous mites * new species * morphology * taxonomy * Madagascar Subject RIV: EG - Zoology Impact factor: 1.161, year: 2014

  2. New species of .i.Euphthiracarus./i. Ewing, 1917 (Acari: Oribatida: Euphthiracaroidea) from the Afrotropical Region

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2014-01-01

    Roč. 64, č. 3 (2014), s. 485-493 ISSN 0003-4541 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : oribatid mites * Euphthiracaroidea * new species * Tanzania * Madagascar Subject RIV: EG - Zoology Impact factor: 1.161, year: 2014

  3. New species of the superfamily Euphthiracaroidea (Acari, Oribatida) from Madagascar and Tanzania

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2015-01-01

    Roč. 49, 27/28 (2015), s. 1689-1702 ISSN 0022-2933 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Oribatida * ptyctimous mites * Euphthiracaridae * new species * Afrotropical region Subject RIV: EG - Zoology Impact factor: 1.010, year: 2015

  4. Uropodina species from the Montagne d' Ambre National Park, Madagascar (Acari: Mesostigmata)

    Czech Academy of Sciences Publication Activity Database

    Kontschán, J.; Starý, Josef

    2012-01-01

    Roč. 119, č. 1 (2012), s. 89-98 ISSN 0035-418X Institutional research plan: CEZ:AV0Z60660521 Keywords : acarology * turtle mites * taxonomy * Afrotropical region Subject RIV: EH - Ecology, Behaviour Impact factor: 0.382, year: 2012

  5. New species of the superfamily Phthiracaroidea (Acari, Oribatida) from the Afrotropical Region

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2015-01-01

    Roč. 56, č. 1 (2015), s. 39-49 ISSN 1681-5556 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Tanzania * Madagascar * Phthiracaridae * taxonomy * new species. Subject RIV: EG - Zoology Impact factor: 0.708, year: 2015

  6. The next new species of .i.Notophthiracarus./i. Ramsay, 1966 (Acari, Oribatida, Phthiracaroidea) from Madagascar

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2015-01-01

    Roč. 56, č. 1 (2015), s. 63-73 ISSN 1681-5556 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Madagascar * new species * oribatid mites * Phthiracaridae * taxonomy Subject RIV: EG - Zoology Impact factor: 0.708, year: 2015

  7. New and little known oribatid mites from Madagascar (Acari: Oribatida. I.

    Directory of Open Access Journals (Sweden)

    Mahunka, S.

    2010-06-01

    Full Text Available A list of the newly studied and identified oribatids from Madagascar (Malagasy Republic is given. Altogether 17species are mentioned from several sites of the island including four new species and a new subspecies belonging in the familiesSteganacaridae, Oppiidae and Austrachipteriidae, respectively. Two species, Eniochthonius sumatranus Mahunka, 1989 andCultroribula bicuspidata Mahunka, 1978 are recorded for the first time from Madagascar. With 25 figures.

  8. Contribution to the knowledge of ptyctimous mites (Acari: Oribatida) from Madagascar

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2013-01-01

    Roč. 59, č. 4 (2013), s. 337-345 ISSN 1217-8837 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : oribatid mites * new species * Mahunka * Phthiracaroidea * Euphthiracaroidea Subject RIV: EG - Zoology Impact factor: 0.263, year: 2013

  9. New species of Atropacarus (.i.Hoplophorella./i.), (Acari, Oribatida, Phthiracaridae) from the Afrotropical Region

    Czech Academy of Sciences Publication Activity Database

    Niedbala, W.; Starý, Josef

    2014-01-01

    Roč. 3774, č. 1 (2014), s. 74-82 ISSN 1175-5326 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Oribatid * ptyctimous mites * new species * taxonomy * morphology Subject RIV: EG - Zoology Impact factor: 0.906, year: 2014

  10. Efeitos do nim sobre tetranychus urticae Koch (Acari: Tetranychidae e os predadores Phytoseiulus macropilis (Banks e Neoseiulus californicus (Mcgregor (Acari: Phytoseiidae Effects of neem on tetranychus urticae Koch (Acari: Tetranychidae and the predators Phytoseiulus macropilis (Banks and Neoseiulus Californicus (Mcgregor (Acari: phytoseiidae

    Directory of Open Access Journals (Sweden)

    Daniele Cristine Hoffmann Schlesener

    2013-03-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficiência de controle e os efeitos adversos de dois produtos à base de nim Azamax® (Azadiractina A/B 12g/L e Neemseto® (Azadiractina A/B, Nimbina e Salanina 2,389 g/L sobre o ácaro-rajado Tetranychus urticae e os predadores Phytoseiulus macropilis e Neoseiulus californicus em laboratório. Para o ácaro-rajado, foram consideradas as variáveis mortalidade, fecundidade, efeito ovicida e persistência biológica, enquanto para os fitoseídeos consideraram-se mortalidade e fecundidade. A mortalidade máxima observada para o ácaro-rajado foi de 89,7% e 91,5% para Azamax® e Neemseto®, respectivamente, na concentração de 0,5% após a reaplicação do produto no sétimo dia. Também foram observados efeitos adversos sobre a fecundidade e a viabilidade dos ovos quando tratados com os produtos comerciais (p.c.. A persistência biológica dos produtos foi de aproximadamente três dias após a pulverização. As formulações apresentaram seletividade em relação aos fitoseídeos, porém causaram redução da fecundidade dos mesmos.The aim of the present study was to evaluate the efficiency of control and adverse effects of two neem based products: Azamax TM (Azadirachtin A/B 12g/L and Neemseto TM (Azadirachtin A/B, Nimbin and Salanin 2,389 g/L over two-spotted spider mite Tetranychus urticae and the predators Phytoseiulus macropilis and Neoseiulus californicus in laboratory. Effects over the mortality, fecundity, eggs and biological persistence of the two-spotted spider mite when treated with neem based products were evaluated. For the phytoseiids the effects on mortality and fecundity were evaluated. The maximum mortality of two-spotted spider mites rates observed were 89.7% and 91.5% for Azamax TM and Neemseto TM respectively, on the 0.5% concentration after reapplying the product on the seventh day. Adverse effects were also observed over the fecundity and viability of the eggs when treated with commercial products (c.p.. The biological persistence of the products was of approximately three days after being pulverized. The formulations present selectivity in relation to the phytoseiids, however they caused fecundity reduction.

  11. Contribution to the knowledge of the oribatid mite genus Eurostocepheus (Acari, Oribatida, Otocepheidae)

    Czech Academy of Sciences Publication Activity Database

    Ermilov, S.G.; Starý, Josef

    2017-01-01

    Roč. 22, č. 5 (2017), s. 640-652 ISSN 1362-1971 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Eurostocepheus * generic diagnosis * morphology * otocepheid mites * Tam Dao National Park * systematics Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 1.467, year: 2016

  12. Feather mites (Acari, Astigmata) associated with birds in an Atlantic Forest fragment in Northeastern Brazil

    OpenAIRE

    Silva, HM; Hernandes, FA; Pichorim, M

    2015-01-01

    AbstractThe present study reports associations between feather mites (Astigmata) and birds in an Atlantic Forest fragment in Rio Grande do Norte state, in Brazil. In the laboratory, mites were collected through visual examination of freshly killed birds. Overall, 172 individuals from 38 bird species were examined, between October 2011 and July 2012. The prevalence of feather mites was 80.8%, corresponding to 139 infested individuals distributed into 30 species and 15 families of hosts. Fiftee...

  13. Ácaros de penas (Acari: Astigmata) em aves não passeriformes do Brasil

    OpenAIRE

    Pedroso, Luiz Gustavo de Almeida [UNESP

    2015-01-01

    Feather mites are the most diverse fauna of arthropods that permanently lives on birds. These mites are morphologicaly adapted to four different microhabitats on their host feathers, which contributes to their diversity. There is a high specificity between the mites and their bird hosts, especially due to the transmission process that occurs mainly from parents to offspring during the parental care, so that each bird group often hosts a specific feather mite fauna, exposing the parallel evolu...

  14. Feather mites (Acari: Astigmata) on birds of Cerrado in Distrito Federal, Brazil

    OpenAIRE

    Kanegae, Mieko Ferreira; Valim, Michel; Fonseca, Marcelo Andrade da; Marini, Miguel Ângelo; Freire, Nicolau Maués Serra

    2008-01-01

    O objetivo do estudo foi identificar os ácaros plumícolas em aves do cerrado. O mesmo foi realizado na Fazenda Água Limpa (FAL), Distrito Federal, Brasil, entre janeiro e agosto de 2002. As aves foram capturadas com rede de neblina e anilhadas. Foram amostradas 696 aves pertencentes a 83 espécies e 25 famílias de Passeriformes e não-Passeriformes. Foram encontrados ácaros plumícolas pertencentes a cinco famílias: Analgidae, Trouessartiidae, Proctophyllodidae, Avenzoariidae e Psoroptoididae. O...

  15. Effect of Temperature on Feeding Period of Larval Blacklegged Ticks (Acari: Ixodidae) on Eastern Fence Lizards.

    Science.gov (United States)

    Rulison, Eric L; Lebrun, Roger A; Ginsberg, Howard S

    2014-11-01

    Ambient temperature can influence tick development time, and can potentially affect tick interactions with pathogens and with vertebrate hosts. We studied the effect of ambient temperature on duration of attachment of larval blacklegged ticks, Ixodes scapularis Say, to eastern fence lizards, Sceloporus undulatus (Bosc & Daudin). Feeding periods of larvae that attached to lizards under preferred temperature conditions for the lizards (WARM treatment: temperatures averaged 36.6°C at the top of the cage and 25.8°C at the bottom, allowing behavioral thermoregulation) were shorter than for larvae on lizards held under cool conditions (COOL treatment temperatures averaged 28.4°C at top of cage and 24.9°C at the bottom). The lizards were infested with larvae four times at roughly monthly intervals. Larval numbers successfully engorging and dropping declined and feeding period was longer after the first infestation. © 2014 Entomological Society of America.

  16. Revealing the diversity of a once small taxon: the genus Selenoribates (Acari, Oribatida, Selenoribatidae

    Directory of Open Access Journals (Sweden)

    Tobias Pfingstl

    2013-06-01

    Full Text Available Three new intertidal oribatid species, Selenoribates elegans sp. n., Selenoribates quasimodo sp. n. and Selenoribates satanicus sp. n. are described from the archipelago of Bermuda. Selenoribates elegans sp. n. is characterized by its slender body shape, S. quasimodo sp. n. possesses a hunchback in lateral view and S. satanicus sp. n. exhibits two horn-like projections on its anterior gastronotic region. Based on these new findings, the number of Selenoribates species doubled at once and the distribution of this genus, formerly limited to the Mediterranean and the Red Sea, includes now occurrences in the Atlantic and Indo-pacific Ocean as well. The morphology of S. quasimodo sp. n. and S. satanicus sp. n. deviates conspicuously from the other known members of Selenoribates, thus indicating that not only the number of species but also the anatomy of this genus is more diverse than formerly supposed. Nymphs of S. quasimodo sp. n. show an interesting case of ontogenetic neotrichy, with gastronotic setae being duplicated with each moult.

  17. Binomial and enumerative sampling of Tetranychus urticae (Acari: Tetranychidae) on peppermint in California.

    Science.gov (United States)

    Tollerup, Kris E; Marcum, Daniel; Wilson, Rob; Godfrey, Larry

    2013-08-01

    The two-spotted spider mite, Tetranychus urticae Koch, is an economic pest on peppermint [Mentha x piperita (L.), 'Black Mitcham'] grown in California. A sampling plan for T. urticae was developed under Pacific Northwest conditions in the early 1980s and has been used by California growers since approximately 1998. This sampling plan, however, is cumbersome and a poor predictor of T. urticae densities in California. Between June and August, the numbers of immature and adult T. urticae were counted on leaves at three commercial peppermint fields (sites) in 2010 and a single field in 2011. In each of seven locations per site, 45 leaves were sampled, that is, 9 leaves per five stems. Leaf samples were stratified by collecting three leaves from the top, middle, and bottom strata per stem. The on-plant distribution of T. urticae did not significantly differ among the stem strata through the growing season. Binomial and enumerative sampling plans were developed using generic Taylor's power law coefficient values. The best fit of our data for binomial sampling occurred using a tally threshold of T = 0. The optimum number of leaves required for T urticae at the critical density of five mites per leaf was 20 for the binomial and 23 for the enumerative sampling plans, respectively. Sampling models were validated using Resampling for Validation of Sampling Plan Software.

  18. Seasonal abundance of Aceria carvi (Acari: Eriophyidae) investing commercial caraway fields in the Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Zemek, Rostislav; Kameníková, L.; Zemková-Rovenská, Gabriela; Samek, T.

    2004-01-01

    Roč. 14, - (2004), s. 593-598 ISSN 0393-8131 R&D Projects: GA ČR(CZ) GA522/02/1490 Institutional research plan: CEZ:AV0Z5007907 Keywords : Aceria carvi * eriophyoid mite * caraway Subject RIV: EH - Ecology, Behaviour

  19. Two new species of the genus Arrenurus from Pohnpei, Federated States of Micronesia (Acari: Hydrchnidia: Arrenuridae)

    Science.gov (United States)

    Harry Smit

    2010-01-01

    Few specimens of water mites have been collected from Pacific Island streams, especially higher elevation, head water streams. Uchida (1935, 1939) and Cook & Bright (1983) published 11 species of water mites from the Palau Islands, while Cook (1957) reported two Arrenurus species from the main island of Yap in the Caroline Island chain. Viets (...

  20. Environmentally associated ticks (Acari: Ixodidae) in Campo Grande, Mato Grosso do Sul, Brazil.

    Science.gov (United States)

    Garcia, Marcos Valério; Silva, Dayana Campelo da; Almeida, Robson Ferreira Cavalcante de; Cunha, Rodrigo Casquero; Matias, Jaqueline; Barros, Jacqueline Cavalcante; Andreotti, Renato; Szabó, Matias Pablo Juan

    2013-01-01

    Herein, we report tick species found on wild and domestic animals and in the environment during a one-year sampling period at the Brazilian Farming Research Company beef cattle unit (Embrapa Beef Cattle), which is located within the urban area of Campo Grande, Mato Grosso do Sul, Brazil. From 55 wild hosts including six different species (Nasua nasua, Cebus spp., Cerdocyon thous, Myrmecophaga tridactyla, Tamandua tetradactyla and Dasyprocta aguti), 323 ticks were collected. Amblyomma ovale ticks were found solely on coatis, and Amblyomma nodosum was identified solely on anteaters. No ticks were found on capuchin monkeys. However, Amblyomma cajennense was found on all parasitized host species with the exception of capuchin monkeys. Giant anteaters displayed the highest infestation abundance, with a mean of 53 ticks∕animal. Environmental sampling yielded 166 adult A. cajennense ticks. The tick species found on domestic animals (Rhipicephalus (Boophilus) microplus, R. sanguineus, Dermacentor nitens and A. cajennense) were those typically found on these hosts in Brazil. The most prevalent tick species, A. cajennense, was found on both wild and domestic animals and was also prevalent in the environment. Thus, this tick species is the primary vector that allows pathogens to bridge wild and domestic animals in the Cerrado.

  1. Ticks (Acari: Ixodidae on wild animals from the Porto-Primavera Hydroelectric power station area, Brazil

    Directory of Open Access Journals (Sweden)

    Marcelo B Labruna

    2002-12-01

    Full Text Available From June 2000 to June 2001, a total of 741 ticks were collected from 51 free-living wild animals captured at the Porto-Primavera Hydroelectric power station area, located alongside an approximately 180 km course of the Paraná river, between the states of São Paulo and Mato Grosso do Sul, comprising 9 species of 3 genera: Ambly-omma (7 species, Boophilus (1 and Anocentor (1. A total of 421 immature Amblyomma ticks were reared in laboratory until the adult stage, allowing identification of the species. A. cajennense was the most frequent tick species (mostly immature stages collected on 9 host species: Myrmecophaga tridactyla, Tamandua tetradactyla,Cerdocyon thous, Puma concolor,Tayassu tajacu, Mazama gouazoubira,Hydrochaeris hydrochaeris,Alouatta caraya, Cebus apella. Other tick species were less common, generally restricted to certain host taxa.

  2. Ticks (Acari: Ixodidae) on wild animals from the Porto-Primavera Hydroelectric power station area, Brazil.

    Science.gov (United States)

    Labruna, Marcelo B; de Paula, Cátia D; Lima, Thiago F; Sana, Dênis A

    2002-12-01

    From June 2000 to June 2001, a total of 741 ticks were collected from 51 free-living wild animals captured at the Porto-Primavera Hydroelectric power station area, located alongside an approximately 180 km course of the Paran river, between the states of S o Paulo and Mato Grosso do Sul, comprising 9 species of 3 genera: Ambly-omma (7 species), Boophilus (1) and Anocentor (1). A total of 421 immature Amblyomma ticks were reared in laboratory until the adult stage, allowing identification of the species. A. cajennense was the most frequent tick species (mostly immature stages) collected on 9 host species: Myrmecophaga tridactyla, Tamandua tetradactyla,Cerdocyon thous, Puma concolor,Tayassu tajacu, Mazama gouazoubira,Hydrochaeris hydrochaeris,Alouatta caraya, Cebus apella. Other tick species were less common, generally restricted to certain host taxa.

  3. Influence of gamma radiation for controlling Brevipalpus phoenicis (Acari: Tenuipalpidae) (Geijskes, 1939) in oxygen atmosphere

    International Nuclear Information System (INIS)

    Machi, Andre R.; Arthur, Valter

    2013-01-01

    Brevipalpus phoenicis mite are controlled across of solutions acaricides, which are chemicals and leave residues in addition there is the difficulty of an effective pulverization due to the small size of the mite, the objective of this study was to evaluate of the influence of oxygen combined with gamma radiation on B.phoenicis as alternative control. Were used 70 mites per arena in 9 reps on 3 treatments at doses of 0 (control), 200 and 300 Gy. For irradiation, the leaves containing the mites, were cut and placed on bottles with bladder tied with ribbons and strings, before was put pure oxygen and the bottle was then sealed, these were taken to a gamma irradiator of Cobalt 60-type Gammacell 220, under a dose rate of 0.381 kGy/hour located in the CENA/USP. Was evaluated daily (eggs, nymphs and adults) of the mites observed viability, fertility and mortality across of the analysis of variance design with completely randomized design using the Statistical Analysis System (SAS) version 9.2® and by the Tukey test, the verification of means. After 22 days of irradiation the hatchability in 200 Gy dose was 41% after 3 days and 57% in control dose, this differed statistically of the other doses, where the nymphs arrived to the adult stage, which did not occurred in the 200 Gy dose and higher due to mutations, generated by the gamma radiation. In 300 Gy not was observed the presence of nymphs and eggs, being the sterilizing dose for all stages of the B.phoenicis. (author)

  4. TOXICIDADE DE ÓLEOS ESSENCIAIS PARA O CONTROLE DE Tetranychus urticae KOCH, 1836 (ACARI: TETRANYCHIDAE)

    OpenAIRE

    Camila Santos Teixeira; Camila Costabeber Nunes; Romário Vargas; José Romário de Carvalho; Hugo Bolsoni Zago

    2017-01-01

    O ácaro rajado é considerado uma importante praga agrícola, causando danos em diferentes cultivos. O controle à base de acaricidas químicos pode vir a causar problemas ambientais e à saúde do homem. Desta forma o uso de óleos essenciais obtidos de produtos botânicos é uma alternativa no controle de pragas por apresentar baixo efeito residual, sendo objetivo deste trabalho avaliar a mortalidade e o efeito na fertilidade de fêmeas adultas de Tetranychus urticae submetidas a tratamentos com óleo...

  5. TOXICIDADE DE ÓLEOS ESSENCIAIS PARA O CONTROLE DE Tetranychus urticae KOCH, 1836 (ACARI: TETRANYCHIDAE

    Directory of Open Access Journals (Sweden)

    Camila Santos Teixeira

    2017-04-01

    Full Text Available O ácaro rajado é considerado uma importante praga agrícola, causando danos em diferentes cultivos. O controle à base de acaricidas químicos pode vir a causar problemas ambientais e à saúde do homem. Desta forma o uso de óleos essenciais obtidos de produtos botânicos é uma alternativa no controle de pragas por apresentar baixo efeito residual, sendo objetivo deste trabalho avaliar a mortalidade e o efeito na fertilidade de fêmeas adultas de Tetranychus urticae submetidas a tratamentos com óleos essenciais de laranja doce e limoneno, fornecidos pela empresa Ferquima Indústria e Comércio LTDA., e pela empresa Pack Indústria e Comércio de Produtos Agropecuários LTDA., respectivamente. Para avaliar a ação fumigante do óleo essencial sobre os ácaros foram realizados testes submetendo fêmeas adultas de T. urticae à dose pura do óleo essencial utilizando as dosagens de 5, 15 e 25 µL em câmaras de fumigação. Após o período de exposição ao óleo foi contabilizado o número de ácaros mortos e a quantidade de ovos depositados. Os resultados não foram significativos para mortalidade e para fertilidade de fêmeas adultas de T. urticae.

  6. Levels of natural resistance to Boophilus microplus (Acari: Ixodidae in Carora breed bulls

    Directory of Open Access Journals (Sweden)

    Roy D. Meléndez

    1998-09-01

    Full Text Available Boophilus microplus infestation is one of the most serious limitations to cattle industry in tropical regions, even though bovines show natural resistance to ticks. This resistance was evaluated in Cross-bred Carora Bulls (CCB a tropicalized dairy breed from Venezuela. Seven CCB were experimentally infested with B. microplus larvae, "Mozo" strain, they were considered tick-naive because they had never been infested with ticks. The mean inoculum size applied on each bull was 6 477 larvae. After life cycle was completed adult female body weight (BW, egg mass weight (EW, egg hatching rate (%EH, and reproductive index (RI were recorded. Results revealed a high variability in the levels of resistance to B. microplus. Thus, one animal showed greater resistance (Dunnett, p< 0.05 for the analyzed parameters in contrast with three non-resistant bulls. The others had moderate resistance. The trait "resistance" should be included togheter with other traits often used in genetic selection of cattle.

  7. Descriptions of two new species of the family oribotritiidae (Acari: Oribatida: Euphthiracaroidea)

    Czech Academy of Sciences Publication Activity Database

    Liu, D.; Niedbala, W.; Starý, Josef

    2011-01-01

    Roč. 61, č. 4 (2011), s. 811-816 ISSN 0003-4541 Grant - others:National Natural Science Foundation of China(CN) 31101617; Chinese Academy of Sciences(CN) KSCX2-EW-Z-8; National Natural Science Foundation of China(CN) 31093430; MNiSW(PL) N N303 201935 Institutional research plan: CEZ:AV0Z60660521 Keywords : oribatid mite * Oribotritiidae * new species Subject RIV: EH - Ecology, Behaviour Impact factor: 0.482, year: 2011

  8. The Ixodes (Acari: Ixodidae) of Mexico: Parasite-Host and Host-Parasite Checklists

    Science.gov (United States)

    2007-08-20

    en Peligro de Extinción. Comisión Nacional para el Cono- cimiento y Uso de la Biodiversidad, Fondo de Cultura Económica, Instituto de Ecología, UNAM...the largest genus of ticks in the world, currently comprising 243 species (Guglielmone et al. 2006), more than one quarter of the global tick fauna ...include it in the Mexican tick fauna . Ixodes bequaerti Cooley and Kohls Previous record CHIAPAS: 1&, Catharinus (sic), 4-V-1942, NA [Cooley & Kohls, 1945

  9. Experimental infection of the bat tick Carios fonsecai (Acari: Ixodidae with the rabies virus

    Directory of Open Access Journals (Sweden)

    Silvana Regina Favoretto

    2013-12-01

    Full Text Available Introduction This study assessed the viability of the rabies virus in the argasid tick Carios fonsecai following experimental infection. Methods The mouse inoculation test (MIT, fluorescent antibody test (FAT and polymerase chain reaction (PCR were used. The rabies virus was administered to ticks via the intra-coelomic route, and the ticks were sacrificed at different time points. Results The inoculated ticks were negative for rabies according to the MIT. Ticks macerated with rabies virus were positive according to the MIT and FAT. All of the tick lots tested by PCR were positive. Conclusions The rabies virus became unviable shortly after its inoculation into tick bodies. Ticks are not likely to play an important role in the epidemiology of rabies.

  10. The male genital accessory gland complex of the cattle tick Boophilus microplus (Canestrini, 1887) (Acari: Ixodidae)

    OpenAIRE

    GARCIA-FERNANDEZ, CASIMIRO; GARCIA, SONIA M. LAUER DE; GARCIA, ROSANE NUNES

    1998-01-01

    A topographical and histological study of the male genital accessory gland complex of Boophilus microplus was undertaken. Ten lobes were found, the most prominent of which is the single dorso-median lobe, subdivided into antero-dorsal and postero-dorsal lobes. The other lobes are: a pair of postero-ventral lobes, a pair of lateral lobes (subdivided into dorso-lateral and postero-lateral lobes), a pair of antero-ventral lobes, a single medio-ventral lobe and a pair of latero-ventral lobes. The...

  11. Application of gamma radiation on longevity of some mites species (Acari: Tetranychidade)

    International Nuclear Information System (INIS)

    Machi, Andre R.; Arthur, Valter

    2015-01-01

    Mites are pests agricultural found in various environments accessible to animal life: soil, aerial parts of the plants, host insects. In this research the effects of gamma irradiation on longevity of mite pests of the tetranychidae family have been studied. The mites were irradiated in a source of Cobalt-60, Gammacell-220 type, at a dose rate of 0.486 kGy located in the CENA/USP, in the doses of 0 (control), 100, 200, 300, and 400 Gy with sixteen replicates per dose. After the irradiation, the mites were placed in petri dishes totalizing 5 treatments in 32 repetitions. The analysis of variance design with completely randomized design using the Statistical Analysis System (SAS) and the Tukey test, the verification of means. Were evaluated daily the adult mortality and longevity of irradiated mites. After 25 days was observed a mean longevity of mites, for O.ilicis, 100 Gy was equal the control dose (18.3 days), but to T. desertorum and T. urticae the larger longevity was observed in the dose of 200 Gy (19.0 days) being that this dose, obtained the larger longevity in comparison to control dose (18.5 days), in general the longevity decreased in relation to increased doses. Thus, only the dose of 100 Gy and 200 Gy stimulated an increased the longevity in O. ilicis and T. desertorum and T. urticae respectively. The exact mechanism by which the mites are tolerant to avoid damage caused by radicals when exposed to ionizing radiation is not fully understood. (author)

  12. Wild birds as hosts of Amblyomma cajennense (Fabricius, 1787 (Acari: Ixodidae

    Directory of Open Access Journals (Sweden)

    Rosario Rojas

    1999-05-01

    Full Text Available We evaluated the prevalence, mean intensity and relative density of ticks in 467 wild birds of 67 species (12 families from forest and cerrado habitats at two protected areas of Minas Gerais, between March and September 1997. Ticks collected (n=177 were identified as larvae and nymphs of Amblyomma cajennense and four other species of Amblyomma. We report for the first time 28 bird species as hosts of the immature stages of A. cajennense, demonstrating the lack of host specificity of the larvae and nymphs. A. cajennense had 15% prevalence on birds, with a mean infestation intensity of 0.37 ticks per host sampled, and 2.5 ticks per infested bird. Prevalence varied in relation to host species, diet and between birds from forests at two successional stages. There were no differences in relation to host forest dependence, participation in mixed flocks of birds, and nest type constructed. A. cajennense is a species of medical and veterinary importance, occurring on domestic animals but is known little of its occurrence on wildlife.

  13. Mite (Acari) fauna of some cultivated plants from Kahramanmaraş ...

    African Journals Online (AJOL)

    user

    2011-03-14

    Mar 14, 2011 ... and fruit trees, the common weed Ipomoea indica (Burman) Merrill ... Mites were removed from the ... 2004). This species was also recorded from the bark of ..... the family Tetranychidae, damaging fruit trees in Central Anatolia.

  14. Application of gamma radiation on longevity of some mites species (Acari: Tetranychidade)

    Energy Technology Data Exchange (ETDEWEB)

    Machi, Andre R., E-mail: rica_machi@hotmail.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Arthur, Valter, E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)

    2015-07-01

    Mites are pests agricultural found in various environments accessible to animal life: soil, aerial parts of the plants, host insects. In this research the effects of gamma irradiation on longevity of mite pests of the tetranychidae family have been studied. The mites were irradiated in a source of Cobalt-60, Gammacell-220 type, at a dose rate of 0.486 kGy located in the CENA/USP, in the doses of 0 (control), 100, 200, 300, and 400 Gy with sixteen replicates per dose. After the irradiation, the mites were placed in petri dishes totalizing 5 treatments in 32 repetitions. The analysis of variance design with completely randomized design using the Statistical Analysis System (SAS) and the Tukey test, the verification of means. Were evaluated daily the adult mortality and longevity of irradiated mites. After 25 days was observed a mean longevity of mites, for O.ilicis, 100 Gy was equal the control dose (18.3 days), but to T. desertorum and T. urticae the larger longevity was observed in the dose of 200 Gy (19.0 days) being that this dose, obtained the larger longevity in comparison to control dose (18.5 days), in general the longevity decreased in relation to increased doses. Thus, only the dose of 100 Gy and 200 Gy stimulated an increased the longevity in O. ilicis and T. desertorum and T. urticae respectively. The exact mechanism by which the mites are tolerant to avoid damage caused by radicals when exposed to ionizing radiation is not fully understood. (author)

  15. New species of water mites from Oman, with some zoogeographical notes (Acari: Hydrachnidia)

    NARCIS (Netherlands)

    Smit, H.; Pešić, V.

    2010-01-01

    The paper deals with a collection of epigean water mites from Oman. One new genus, Omanaxonopsis, and 16 new species are described: Bharatavolzia arabica, Nilotonia bisetosa, N. longiseta, Torrenticola arabica, T. omanensis, Limnesia kochi, Protolimnesia inexspectata, Atractides arabicus, A.

  16. Efficacy of granular deltamethrin against Ixodes scapularis and Amblyomma americanum (Acari: Ixodidade) nymphs.

    Science.gov (United States)

    Schulze, T L; Jordan, R A; Hung, R W; Taylor, R C; Markowski, D; Chomsky, M S

    2001-03-01

    A single barrier application of granular deltamethrin to the woodland edges of a forested residential community in late spring significantly reduced the abundance of Ixodes scapularis Say nymphs. The application also suppressed the population of Amblyomma americanum (L.) nymphs, which recently became established in the study area. The efficacy of deltamethrin is compared with other commonly used acaricides.

  17. Three Species of Ectoparasite Mites (Acari: Pterygosomatidae Infested Geckos in Indonesia

    Directory of Open Access Journals (Sweden)

    TARUNI SRI PRAWASTI

    2013-06-01

    Full Text Available Limited data is hitherto available on the diversity and dispersal of parasitic mites of geckos in Indonesia. Here, we collected three species of geckos, namely Cosymbotus platyurus, Hemidactylus frenatus, and H. garnotii throughout Indonesia to study the distribution and diversity of its parasitic mites. We conducted detail morphological analysis of the mites using whole mount polyvinyl lactophenol and scanning electron microscope preparation. Three species of ectoparasite mites from genus Geckobia were identified in a total of 221 individuals out of 448 geckos collected from 25 sites in Indonesia. Two species were G. glebosum and G. bataviensis, and the other one was designated as Geckobia sp 1. Based on our result, the three mites species were spread randomly and live sympatrically. The G. bataviensis mite showed the widest distribution, because it was found in almost all gecko collection sites, hence the most cosmopolitan mites. We also found that C. platyurus gecko had the lowest mite prevalence which might due to the fact that it has the least number of skin folds, an important site for mite protection. This result implies that further research on the relationship of anatomy of gecko skin with chelicera and claw structure of mites is necessary in the future.

  18. Genetic Diversity of Salp15 in the Ixodes ricinus Complex (Acari: Ixodidae)

    OpenAIRE

    Wang, Xin; Huang, Yong; Niu, Si-bo; Jiang, Bao-Gui; Jia, Na; van der Geest, Leo; Ni, Xue-bing; Sun, Yi; Cao, Wu-Chun

    2014-01-01

    Salp15, a 15-kDa tick salivary gland protein, is both essential for ticks to successfully obtain host blood and also facilitates transmission of Lyme borreliosis. To determine whether the Salp15 gene is expressed in Ixodes persulcatus and Ixodes sinensis, principle vectors of Lyme borreliosis in China, we studied transcriptions of this gene in semi-engorged larvae, nymph and adults of these two species. A total of eight Salp15 homologues, five in I. persulcatus and three in I. sinensis, were ...

  19. Comparative Study of Feeding on Some Pollens on Biology of Typhlodromus bagdasarjani Arutunjian & Wainstein (Acari: Phytoseiidae

    Directory of Open Access Journals (Sweden)

    E. Hajmohammadloo

    2011-06-01

    Full Text Available In this research, effect of feeding of five pollen sources, apple, pear, almond, apricot and walnut, as 5 treatments on the period of egg to adult, longevity and fecundity of the predatory mite, Typhlodromus bagdasarjani, was studied in a complete randomized design (CRD. Mites kept individually on black mulberry leaf discs at 24±2 °C temperature, 60±5 % relative humidity and a photoperiod of 16:8 (L:D hours, with a sufficient quantity of the pollens. Results showed that T. bagdasarjani could develop and reproduce when the predatory mite feed on the all of diets. Thus, the pollens can be alternative foods for the mite. The mean of developmental time from egg to adult emergence varied between the treatments from 11.69 to 21.49 days for females and 11.70 to 20.07 days for males. The minimum mean of developmental time of females and males was on walnut, almond and apple pollens and the maximum mean of it was on pear pollen. The mean of longevity varied among the treatments from 17.45 to 31.26 days for the females and 18.23 to 31.44 days for the male insects. The maximum mean longevity was on apricot and almond pollens for females and it had maximum amount on apricot and walnut for males. The minimum mean of longevity of females and males was on pear pollen. Also the maximum means of daily and total fecundity (0.73 and 9.60 eggs/female were on apple and walnut pollens. The minimum amount of these means was on pear pollen. According to these results, almond and walnut pollens were the most suitable diet for T. bagdasarjani as compared to the other pollens. Among diets, pear pollen had least desirability and nutritional value for the mite.

  20. Amblyomma auricularium (Acari: Ixodidae: underwater survival of the non-parasitic phase of feeding females

    Directory of Open Access Journals (Sweden)

    Iwine Joyce Barbosa de Sá-Hungaro

    Full Text Available To determine the effects of immersion in water on the biological parameters of engorged females of the tick species Amblyomma auricularium, 60 females were distributed in six groups, each comprising 10 individuals. The control group – G1 (not immersed was fixed dorsally in a Petri dish and incubated at 27 ± 1°C and 80% RH. The other groups were subjected to immersion periods of 24, 48, 72 and 96 hours, and the sixth group to continuous immersion. After the immersion period, the females were placed in Petri dishes to begin laying. Eggs were collected every 72 hours and kept in biological chambers. All the groups showed significant differences (p <0.05 during the pre-oviposition period. The laying period and the average weight of overall posture did not change. The egg incubation period also did not differ significantly, but the hatching rate in the group immersed for 96h showed a significant difference. Thus, immersion for up to 96 hours does not impair the survival of A. auricularium females, although it may delay egg laying and reduce the number of offspring.

  1. Repellent activity of fractioned compounds from Chamaecyparis nootkatensis essential oil against nymphal Ixodes scapularis (Acari: Ixodidae).

    Science.gov (United States)

    Dietrich, Gabrielle; Dolan, Marc C; Peralta-Cruz, Javier; Schmidt, Jason; Piesman, Joseph; Eisen, Rebecca J; Karchesy, Joseph J

    2006-09-01

    Preliminary repellent activity of 14 natural products isolated from essential oil components extracted from the heartwood of Alaska yellow cedar, Chamaecyparis nootkatensis (D. Don) Spach., were evaluated against nymphal Ixodes scapularis Say in a laboratory bioassay and compared with technical grade N,N-diethyl-3-methylbenzamide (deet). Four hours after treatment, nootkatone and valencene-13-ol had repellent concentration (RC)50 values of 0.0458 and 0.0712% (wt:vol), respectively; two additional Alaska yellow cedar compounds, nootkatone 1 --> 10 epoxide and carvacrol had reported RC50 values of 0.0858 and 0.112%, respectively. The observed RC50 value for deet was 0.0728% (wt:vol). Although not statistically significantly more active than deet, the ability of these natural products to repel ticks at relatively low concentrations may represent a potential alternative to synthetic commercial repellents.

  2. Feather mites of the subfamily Proctophyllodinae (Acari: Proctophyllodidae) from passerines (Aves: Passeriformes) in Costa Rica

    Czech Academy of Sciences Publication Activity Database

    Mironov, S. V.; Literák, I.; Sychra, O.; Čapek, Miroslav

    2017-01-01

    Roč. 4297, č. 1 (2017), s. 1-105 ISSN 1175-5326 R&D Projects: GA AV ČR IAA601690901; GA MŠk LC06073 Institutional research plan: CEZ:AV0Z60930519 Institutional support: RVO:68081766 Keywords : Proctophyllodidae * systematics * new species * fauna * host associations * Passeriformes * Costa Rica Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 0.972, year: 2016

  3. Life cycle of Amblyomma mixtum (Acari: Ixodidae) parasitizing different hosts under laboratory conditions

    Science.gov (United States)

    Amblyomma mixtum is a tick species in the Amblyomma cajennense complex. The known geographic range of A. mixtum extends from Texas in the USA to western Ecuador and some islands in the Caribbean. Amblyomma mixtum is a vector of disease agents of veterinary and public health importance. The objective...

  4. Successful Feeding of Amblyomma coelebs (Acari: Ixodidae) Nymphs on Humans in Brazil: Skin Reactions to Parasitism.

    Science.gov (United States)

    Garcia, Marcos V; Matias, Jaqueline; Aguirre, AndrÉ De A R; Csordas, Barbara G; SzabÓ, Matias P J; Andreotti, Renato

    2015-03-01

    Identifying the tick species that successfully feed on humans would increase knowledge of the epidemiology of several tick-borne diseases. These species salivate into the host, increasing the risk of pathogen transmission. However, there is a lack of data in the literature regarding the ticks that prefer to feed on humans. Herein, we describe the successful feeding of Amblyomma coelebs Neumann nymphs on two of the authors after accidental tick bites occurred during field surveys in two preserved areas of Mato Grosso do Sul, Brazil. One of the host-parasite interactions was closely monitored, and the tick development, gross host skin alterations, and related sensations are presented. © The Author 2015. Published by Oxford University Press on behalf of Entomological Society of America.

  5. Mitotic and meiotic chromosomes of a southern Brazilian population of Boophilus microplus (Acari, Ixodidae

    Directory of Open Access Journals (Sweden)

    Rosane Nunes Garcia

    Full Text Available Using conventional staining with acetic orcein and C-banding techniques it was investigated constitutive heterochromatin chromosomal polymorphisms and the mitotic and the meiotic behavior of male and female chromosomes of Boophilus microplus (Canestrini, 1887. Some differences were detected in the population of southern Brazil as compared to the data of other authors for populations in other latitudes. The differences being mainly concerned with the distribution of constitutive centromeric heterochromatin and variation in the length of heterochromatic blocks in the pericentromeric regions of some chromosome pairs.

  6. In vitro activity of pineapple extracts (Ananas comosus, Bromeliaceae) on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).

    Science.gov (United States)

    Domingues, Luciana Ferreira; Giglioti, Rodrigo; Feitosa, Karina Alves; Fantatto, Rafaela Regina; Rabelo, Márcio Dias; Oliveira, Márcia Cristina de Sena; Oliveira, Gilson Pereira de; Bechara, Gervasio Henrique; Chagas, Ana Carolina de Souza

    2013-07-01

    Measures to control the cattle tick, Rhipicephalus (Boophilus) microplus, based only on chemical products are becoming unsustainable, mainly because of the development of resistance. The objective of this study was to test the effect of the aqueous extract of pineapple skin (AEPS) and bromelain extracted from the stem (Sigma-Aldrich®, B4882) on engorged females and larvae of R. (B.) microplus in vitro. These substances were diluted in water and evaluated at eight concentrations. Engorged females were collected and distributed in groups of 10, with three repetitions for each treatment. After immersion in the solutions, the females were placed in an incubator for observation of survival, oviposition and larval hatching. The larval packet method was used, also with three repetitions with about 100 larvae each. The packets were incubated and the readings were performed after 24 h. The estimated reproduction and efficacy of the solutions were calculated. The LC(50) and LC(90) were estimated using the Probit procedure of the SAS program. The eight concentrations were compared within each treatment by the Tukey test. For the experiment with engorged females, the most effective concentrations were 125, 250 and 500 mg/mL: 33%, 48% and 59% for the AEPS and 27%, 51% and 55% for the bromelain. The LC(50) and LC(90) values were, respectively, 276 and 8691 mg/mL for AEPS and 373 and 5172 mg/mL for bromelain. None of the dilutions tested was effective against the larvae of R. (B.) microplus. This is the first report of the action of pineapple extracts or their constituents on cattle ticks. The results demonstrate that further studies regarding composition of tick cuticle, with evaluation of other solvents and formulations, should be conducted seeking to enhance the effect of pineapple extracts and compounds against this ectoparasite. Copyright © 2013 Elsevier Inc. All rights reserved.

  7. Stored product mites (Acari: Astigmata) infesting food in various types of packaging.

    Science.gov (United States)

    Hubert, Jan; Nesvorna, Marta; Volek, Vlado

    2015-02-01

    From 2008 to 2014, stored product mites have been reported from prepackaged dried food on the market in the Czech Republic. The infestation was by Carpoglyphus lactis (L.) in dried fruits and Tyrophagus putrescentiae (Schrank) in dog feed. The infestation is presumably caused by poor protection of the packages. We compared various packaging methods for their resistance to mites using dried apricots and dog feed in laboratory experiments. The trial packages included nine different plastic films, monofilm, duplex and triplex, and one type of plastic cup (ten replicates per packaging type). All packaging materials are available on the Czech market for dried food products. The samples of dried food were professionally packed in a factory and packaged dried apricots were exposed to C. lactis and dog food to T. putrescentiae. After 3 months of exposure, the infestation and mite density of the prepackaged food was assessed. Mites were found to infest six types of packages. Of the packaging types with mites, 1-5 samples were infested and the maximum abundance was 1,900 mites g(-1) of dried food. Mites entered the prepackaged food by faulty sealing. Inadequate sealing is suggested to be the major cause of the emerged infestation of dried food.

  8. Three new species of the genus Acanthomastix Manunka, 1972 from United States and Poland (Acari: Dolichocybidae)

    Science.gov (United States)

    Wojciech L. Magowski; John C. Moser

    1993-01-01

    Three new species of the genus Mahunka, 1972 are described and figured: A. and A. elegans spp. n. phoretic on Hylobius pales (Herbst) (Insecta: Curculionidae) from USA and A. minor sp. n. from rotten fir bark in Poland. The genus Acanthomastix...

  9. Susceptibility of Oligonychus yothersi (Acari: Tetranychidae) to the fungus Beauveria bassiana

    OpenAIRE

    Oliveira, Renato Cassol de; Alves, Luis Francisco Angeli; Neves, Pedro Manuel Oliveira Janeiro

    2002-01-01

    A cultura da erva-mate (Ilex paraguariensis) é uma importante atividade econômica no Sul do Brasil. Com o aumento de consumo nos anos oitenta, cresceram também as áreas de monocultura, gerando condições favoráveis para o aumento populacional de insetos e ácaros fitófagos, entre eles o ácaro vermelho Oligonychus yothersi. Este estudo avaliou a suscetibilidade do ácaro vermelho O. yothersi a vários isolados do fungo Beauveria bassiana. O experimento foi conduzido em Cascavel, PR. Discos foliare...

  10. Detection of spotted fever group (SFG) rickettsiae in Dermacentor reticulatus (Acari: Ixodidae) in Poland.

    Science.gov (United States)

    Stańczak, Joanna

    2006-05-01

    Dermacentor reticulatus ticks from Poland were investigated by molecular methods for the presence of rickettsiae. During 2003/2004, a total of 285 adult ticks was assayed using primers RpCS.877 and RpCS.1258 derived from the citrate synthase (gltA) gene, and 116 samples (40.7%) were positive for rickettsial DNA. Ten out of these positive samples were further assayed using SLO1F and SLO1R primers derived form the rOmpA-encoding gene to confirm that detected rickettsiae belong to the spotted fever group (SFG). The obtained sequence of a fragment of the gltA gene of Rickettsia sp. isolated from Polish D. reticulatus demonstrated 96-98% similarities to Rickettsia slovaca, Rickettsia sibirica, Rickettsia honei, and other SFG rickettsiae. The nucleotide sequences of the amplified fragments of the ompA gene were 98% homologous to RpA4 Rickettsia sp. reported from ticks collected in territories of the former Soviet Union.

  11. Detection of a Novel Rickettsia From Leptotrombidium scutellare Mites (Acari: Trombiculidae) From Shandong of China.

    Science.gov (United States)

    Huang, Yuting; Zhao, Li; Zhang, Zhentang; Liu, Miaomiao; Xue, Zaifeng; Ma, Dongqiang; Sun, Xifeng; Sun, Yue; Zhou, Chuanmin; Qin, Xiangrong; Zhu, Yelei; Li, Wenqian; Yu, Hao; Yu, Xue-Jie

    2017-05-01

    Leptotrombidium scutellare mites, the vector of Orientia tsutsugamushi, have rarely been reported to associate with Rickettsia species. Three hundred nineteen chiggers were collected from the ears of 32 rodents captured in Huangdao District of Qingdao City, China, in October 2015. The chigger samples were tested for Rickettsia, severe fever with thrombocytopenia syndrome virus, and hantavirus by PCR or RT-PCR amplification. All mites were classified morphologically and molecularly as L. scutellare chiggers. Rickettsial DNA sequences were amplified for four genes including 16S rRNA, ompB, gltA, and 17 kD protein genes. The minimum infection rate (MIR; number of positive pools/total specimens tested) of the Rickettsia species in the chiggers were 2.8% (9/319). Phylogenetic analysis indicated that individual genes were closely related to different Rickettsia species including R. felis (with 16S rRNA gene), R. australis (with gltA gene), an unnamed Rickettsia sp. TwKM02 (with ompB gene), and Rickettsia endosymbiont of soft tick Ornithodoros erraticus (with 17 kD protein gene). Phylogenic analysis of the concatenated sequence of 16S rRNA, gltA, ompB, and 17 kD protein genes indicated that the Rickettsia species from L. scutellare chigger was most closely related to R. australis and R. akari. These results indicated that the Rickettsia species in chiggers was unique; it was named Candidatus Rickettsia leptotrombidium. Severe fever with thrombocytopenia syndrome virus and hantavirus were not amplified from the chiggers, suggesting lack of infection of these pathogens in the chiggers. A unique Rickettsia species was detected in L. scutellare, which expanded the knowledge on the vector distribution of Rickettsia. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  12. Molecular Detection of Rickettsia Species Within Ticks (Acari: Ixodidae) Collected from Arkansas United States.

    Science.gov (United States)

    Trout Fryxell, R T; Steelman, C D; Szalanski, A L; Billingsley, P M; Williamson, P C

    2015-05-01

    Rocky Mountain spotted fever (RMSF), caused by the etiological agent Rickettsia rickettsii, is the most severe and frequently reported rickettsial illness in the United States, and is commonly diagnosed throughout the southeast. With the discoveries of Rickettsia parkeri and other spotted fever group rickettsiae (SFGR) in ticks, it remains inconclusive if the cases reported as RMSF are truly caused by R. rickettsii or other SFGR. Arkansas reports one of the highest incidence rates of RMSF in the country; consequently, to identify the rickettsiae in Arkansas, 1,731 ticks, 250 white-tailed deer, and 189 canines were screened by polymerase chain reaction (PCR) for the rickettsial genes gltA, rompB, and ompA. None of the white-tailed deer were positive, while two of the canines (1.1%) and 502 (29.0%) of the ticks were PCR positive. Five different tick species were PCR positive: 244 (37%) Amblyomma americanum L., 130 (38%) Ixodes scapularis Say, 65 (39%) Amblyomma maculatum (Koch), 30 (9%) Rhipicephalus sanguineus Latreille, 7 (4%) Dermacentor variabilis Say, and 26 (44%) unidentified Amblyomma ticks. None of the sequenced products were homologous to R. rickettsii. The most common Rickettsia via rompB amplification was Rickettsia montanensis and nonpathogenic Candidatus Rickettsia amblyommii, whereas with ompA amplification the most common Rickettsia was Ca. R. amblyommii. Many tick specimens collected in northwest Arkansas were PCR positive and these were commonly A. americanum harboring Ca. R. amblyommii, a currently nonpathogenic Rickettsia. Data reported here indicate that pathogenic R. rickettsii was absent from these ticks and suggest by extension that other SFGR are likely the causative agents for Arkansas diagnosed RMSF cases. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  13. Bacteria of the genus Rickettsia in ticks (Acari: Ixodidae) collected from birds in Costa Rica.

    Science.gov (United States)

    Ogrzewalska, Maria; Literák, Ivan; Capek, Miroslav; Sychra, Oldřich; Calderón, Víctor Álvarez; Rodríguez, Bernardo Calvo; Prudencio, Carlos; Martins, Thiago F; Labruna, Marcelo B

    2015-06-01

    The aim of this study was to document the presence of Rickettsia spp. in ticks parasitizing wild birds in Costa Rica. Birds were trapped at seven locations in Costa Rica during 2004, 2009, and 2010; then visually examined for the presence of ticks. Ticks were identified, and part of them was tested individually for the presence of Rickettsia spp. by polymerase chain reaction (PCR) using primers targeting fragments of the rickettsial genes gltA and ompA. PCR products were DNA-sequenced and analyzed in BLAST to determine similarities with previously reported rickettsial agents. A total of 1878 birds were examined, from which 163 birds (9%) were infested with 388 ticks of the genera Amblyomma and Ixodes. The following Amblyomma (in decreasing order of abundance) were found in immature stages (larvae and nymphs): Amblyomma longirostre, Amblyomma calcaratum, Amblyomma coelebs, Amblyomma sabanerae, Amblyomma varium, Amblyomma maculatum, and Amblyomma ovale. Ixodes ticks were represented by Ixodes minor and two unclassified species, designated here as Ixodes sp. genotype I, and Ixodes sp. genotype II. Twelve of 24 tested A. longirostre ticks were found to be infected with 'Candidatus Rickettsia amblyommii', and 2 of 4 A. sabanerae were found to be infected with Rickettsia bellii. Eight of 10 larval Ixodes minor were infected with an endosymbiont (a novel Rickettsia sp. agent) genetically related to the Ixodes scapularis endosymbiont. No rickettsial DNA was found in A. calcaratum, A. coelebs, A. maculatum, A. ovale, A. varium, Ixodes sp. I, and Ixodes sp. II. We report the occurrence of I. minor in Costa Rica for the first time and a number of new bird host-tick associations. Moreover, 'Candidatus R. amblyommii' and R. bellii were found in A. longirostre and A. sabanerae, respectively, in Costa Rica for the first time. Copyright © 2015 Elsevier GmbH. All rights reserved.

  14. Molecular Detection and Identification of Rickettsia Species in Ticks (Acari: Ixodidae) Collected From Belize, Central America.

    Science.gov (United States)

    Polsomboon, Suppaluck; Hoel, David F; Murphy, Jittawadee R; Linton, Yvonne-Marie; Motoki, Maysa; Robbins, Richard G; Bautista, Kim; Bricen O, Ireneo; Achee, Nicole L; Grieco, John P; Ching, Wei-Mei; Chao, Chien-Chung

    2017-11-07

    Little is known about tick-borne rickettsial pathogens in Belize, Central America. We tested ixodid ticks for the presence of Rickettsia species in three of the six northern and western Belizean districts. Ticks were collected from domestic animals and tick drags over vegetation in 23 different villages in November 2014, February 2015, and May 2015. A total of 2,506 collected ticks were identified to the following species: Dermacentor nitens Neumann (46.69%), Rhipicephalus sanguineus (Latreille) (19.55%), Rhipicephalus microplus (Canestrini) (19.47%), Amblyomma cajennense complex (9.74%), Amblyomma maculatum Koch (3.47%), Amblyomma ovale Koch (0.68%), Ixodes nr affinis (0.16%), Amblyomma nr maculatum (0.12%), and Amblyomma nr oblongoguttatum (0.12%). Ticks were pooled according to species, life stage (larva, nymph, or adult), and location (n = 509) for DNA extraction and screened for genus Rickettsia by quantitative real-time polymerase chain reaction (qPCR). All 42 positive pools were found to be positive for spotted fever group (SFG) Rickettsia in pools of A. cajennense complex (n = 33), A. maculatum (n = 4), A. nr maculatum (n = 1), A. ovale (n = 1), R. sanguineus (n = 1), and I. nr affinis (n = 2). Rickettsia amblyommatis was identified from A. cajennense complex and A. nr maculatum. Rickettsia parkeri was found in A. maculatum, and Rickettsia sp. endosymbiont was detected in I. nr affinis. The presence of infected ticks suggests a risk of tick-borne rickettsioses to humans and animals in Belize. This knowledge can contribute to an effective tick management and disease control program benefiting residents and travelers. Published by Oxford University Press on behalf of Entomological Society of America 2017. This work is written by US Government employees and is in the public domain in the US.

  15. Rickettsia vini n. sp. (Rickettsiaceae) infecting the tick Ixodes arboricola (Acari: Ixodidae).

    Science.gov (United States)

    Novakova, Marketa; Costa, Francisco B; Krause, Frantisek; Literak, Ivan; Labruna, Marcelo B

    2016-08-26

    Recently, a new rickettsia named 'Candidatus Rickettsia vini' belonging to the spotted fever group has been molecularly detected in Ixodes arboricola ticks in Spain, the Czech Republic, Slovakia and Turkey, with prevalence reaching up to 100 %. The aim of this study was to isolate this rickettsia in pure culture, and to describe it as a new Rickettsia species. A total of 148 ornitophilic nidicolous ticks Ixodes arboricola were collected in a forest near Breclav (Czech Republic) and examined for rickettsiae. Shell vial technique was applied to isolate rickettsiae in Vero cells. Rickettsial isolation was confirmed by optical microscopy and sequencing of partial sequences of the rickettsial genes gltA, ompA, ompB, and htrA. Laboratory guinea pigs and chickens were used for experimental infestations and infections. Animal blood sera were tested by immunofluorescence assay employing crude antigens of various rickettsiae. Rickettsia vini n. sp. was successfully isolated from three males of I. arboricola. Phylogenetic analysis of fragments of 1092, 590, 800, and 497 nucleotides of the gltA, ompA, ompB, and htrA genes, respectively, showed closest proximity of R. vini n. sp. to Rickettsia japonica and Rickettsia heilongjiangensis belonging to the spotted fever group. Experimental infection of guinea pigs and chickens with R. vini led to various levels of cross-reactions of R. vini-homologous antibodies with Rickettsia rickettsii, Rickettsia parkeri, 'Candidatus Rickettsia amblyommii', Rickettsia rhipicephali, Rickettsia bellii, and Rickettsia felis. Laboratory infestations by R. vini-infected I. arboricola larvae on chickens led to no seroconversion to R. vini n. sp., nor cross-reactions with R. rickettsii, R. parkeri, 'Ca. R. amblyommii', R. rhipicephali, R. bellii or R. felis. Our results suggest that R. vini n. sp. is possibly a tick endosymbiont, not pathogenic for guinea pigs and chickens. Regarding specific phenotypic characters and significant differences of DNA sequences in comparison to the most closely related species (R. japonica and R. heilongjiangensis), we propose to classify the isolate as a new species, Rickettsia vini.

  16. Detection of a novel Rickettsia sp. in soft ticks (Acari: Argasidae) in Algeria.

    Science.gov (United States)

    Lafri, Ismail; Leulmi, Hamza; Baziz-Neffah, Fadhila; Lalout, Reda; Mohamed, Chergui; Mohamed, Karakallah; Parola, Philippe; Bitam, Idir

    2015-01-01

    Argasid ticks are vectors of viral and bacterial agents that can infect humans and animals. In Africa, relapsing fever borreliae are neglected arthropod-borne pathogens that cause mild to deadly septicemia and miscarriage. It would be incredibly beneficial to be able to simultaneous detect and identify other pathogens transmitted by Argasid ticks. From 2012 to 2014, we conducted field surveys in 4 distinct areas of Algeria. We investigated the occurrence of soft ticks in rodent burrows and yellow-legged gull (Larus michahellis) nests in 10 study sites and collected 154 soft ticks. Molecular identification revealed the occurrence of two different soft tick genera and five species, including Carios capensis in yellow-legged gull nests and Ornithodoros occidentalis, Ornithodoros rupestris, Ornithodoros sonrai, Ornithodoros erraticus in rodent burrows. Rickettsial DNA was detected in 41/154, corresponding to a global detection rate of 26.6%. Sequences of the citrate synthase (gltA) gene suggest that this agent is a novel spotted fever group Rickettsia. For the first time in Algeria, we characterize a novel Rickettsia species by molecular means in soft ticks. Copyright © 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  17. Three new species of the genus Austrophthiracarus from New Zealand (Acari: Oribatida: Phthiracaridae).

    Science.gov (United States)

    Liu, Dong; Zhang, Zhi-Qiang

    2014-03-24

    Three new species of Austrophthiracarus (Oribatida: Phthiracaridae) from New Zealand are described: Austrophthiracarus matuku sp. nov. from the Bethells Matuku Reserve, Auckland, Austrophthiracarus notoporosus sp. nov. from the Tutoko Bench, Fiordland and Austrophthiracarus karioi sp. nov. from the Mt. Karioi, Waikato. Holotype specimens are deposited in the New Zealand Arthropod Collection, Landcare Research and paratypes are deposited in the Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences.

  18. Pathogenicity of entomopathogenic fungus Metarhizium anisopliae (Deuteromycetes) to Ixodes scapularis (Acari: Ixodidae)

    Science.gov (United States)

    Zhioua, E.; Browning, M.; Johnson, P.W.; Ginsberg, H.S.; LeBrun, R.A.

    1997-01-01

    The entomopathogenic fungus Metarhizium anisopliae is highly pathogenic to the black-legged tick, Ixodes scapularis. Spore concentrations of 108/ml for engorged larvae and 107/ml for engorged females resulted in 100% tick mortality, 2 wk post-infection. The LC50 value for engorged larvae (concentration to kill 50% of ticks) was 107 spores/ml. Metarhizium anisopliae shows considerable potential as a microbial control agent for the management of Ixodes scapularis.

  19. Unveiling the oxidative metabolism of Rhipicephalus microplus (Acari: Ixodidae) experimentally exposed to entomopathogenic fungi.

    Science.gov (United States)

    Tunholi-Alves, Vinícius Menezes; Tunholi Alves, Victor Menezes; da Silva, Jairo Pinheiro; Nora Castro, Rosane; Salgueiro, Fernanda Barbosa; Perinotto, Wendell Marcelo de Souza; Gôlo, Patrícia Silva; Camargo, Mariana Guedes; Angelo, Isabele da Costa; Bittencourt, Vânia Rita Elias Pinheiro

    2016-10-01

    Rhipicephalus microplus is an important tick in tropical regions due to the high economic losses caused by its parasitism. Metarhizium anisopliae and Beauveria bassiana are well-known entomopathogenic fungi that can afflict R. microplus ticks. The development of new targets and strategies to control this parasite can be driven by studies of this tick's physiology. Recently, it was reported that when exposed to adverse physiological conditions, ticks can activate fermentative pathways, indicating transition from aerobic to anaerobic metabolism. Nevertheless, the precise mechanism by which entomopathogenic fungi influence R. microplus metabolism has not been clarified, limiting understanding of the tick-fungus association. Thus, the present study aimed to evaluate the effect of infection of ticks by M. anisopliae and B. bassiana on the amount of selected carboxylic acids present in the hemolymph, enabling increased understanding of changes previously reported. The results showed preservation in the concentrations of oxalic, lactic, and pyruvic acids in the hemolymph 24 and 48 h after dropping from cattle; while there were variations in the concentration of these carboxylic acids after infection of female ticks to M. anisopliae and B. bassiana. Significant increases were observed in the concentration of oxalic and lactic acids and significant reduction of pyruvic acid for both observation times (24 and 48 h) after infection by entomopathogenic fungi. These results indicate that B. bassiana and M. anisopliae infection alters the basal metabolism of R. microplus females, resulting in the activation of fermentative pathways.

  20. Pathogenicity of Metarhizium anisopliae (Deuteromycetes) and permethrin to Ixodes scapularis (Acari: Ixodidae) nymphs

    Science.gov (United States)

    Hornbostel, V.L.; Zhioua, Elyes; Benjamin, Michael A.; Ginsberg, Howard S.; Ostfeld, Richard S.

    2005-01-01

    Effectiveness of the entomopathogenic fungus Metarhizium anisopliae, for controlling nymphal Ixodes scapularis, was tested in laboratory and field trials. In the laboratory, M. anisopliae (Metschnikoff) Sorokin strain ESC1 was moderately pathogenic, with an LC50 of 107 spores/ml and induced 70% mortality at 109 spores/ml. In a field study, however, 109 spores/ml M. anisopliae did not effectively control questing I. scapularis nymphs, and significant differences were not detected in pre- and post-treatment densities. For nymphs collected and returned to the laboratory for observation, mortality was low in treatment groups, ranging from 20 to 36%. To assess whether a chemical acaricide would synergistically enhance pathogenicity of the fungus, we challenged unfed nymphal I. scapularis with combinations of M. anisopliae and permethrin, a relatively safe pyrethroid acaricide, in two separate bioassays. Significant interactions between M. anisopliae and permethrin were not observed, supporting neither synergism nor antagonism.

  1. Association between entomopathogenic nematodes and fungi for control of Rhipicephalus microplus (Acari: Ixodidae).

    Science.gov (United States)

    Monteiro, Caio Márcio Oliveira; Araújo, Laryssa Xavier; Matos, Renata Silva; da Silva Golo, Patrícia; Angelo, Isabele Costa; de Souza Perinotto, Wendell Marcelo; Coelho Rodrigues, Camila Aparecida; Furlong, John; Bittencourt, Vânia Rita Elias Pinheiro; Prata, Márcia Cristina Azevedo

    2013-10-01

    The aim of the study was to assess the effect of the association of entomopathogenic nematodes and fungi on Rhipicephalus microplus. The nematodes used were Heterorhabditis bacteriophora HP88 and Heterorhabditis indica LPP1 and the fungi were Metarhizium anisopliae IBCB 116 and Beauveria bassiana ESALQ 986. In the groups treated with the fungi, the females were immersed for 3 min in a conidial suspension, while in the groups treated with the nematodes, the ticks were exposed to infective juveniles. To evaluate the interaction between entomopathogens, the females were first immersed in a conidial suspension and then exposed to the nematodes. The egg mass weight and hatching percentage values of the groups treated with M. anisopliae IBCB 116 and B. bassiana ESALQ 986 in the two experiments were statistically similar (p > 0.05) to the values of the control group. In the groups treated only with nematodes, there was a significant reduction (p fungus M. anisopliae IBCB 116.

  2. Diagrammatic scale of Aceria guerreronis Keifer (Acari: Eriophyidae) damage in coconut

    International Nuclear Information System (INIS)

    Galvao, Andreia S.; Gondim Junior, Manoel G.C.; Michereff, Sami J.

    2008-01-01

    Aceria guerreronis Keifer is an important pest of coconut worldwide. Due to the lack of standardized methods to quantify damage of this eryophyid, a diagrammatic scale with indices of 1, 2, 4, 8, 16, 32, 48 and 70% of damage caused by A. guerreronis was elaborated and tested to accuracy, precision and reproduction of the estimations. To validate the scale, fruits with different levels of damage were previously measured with the program Assess R and submitted to 10 inexperienced evaluators with or without the scale (first evaluation) and seven days after (second evaluation) with the same evaluators, using digitized pictures of the same fruits in a different sequence. The accuracy and precision of each evaluator was determined through linear regression between observed and estimated damage. Without using the scale, evaluators were less precise as seven out of 10 overestimated the damage, while evaluators provided with the scale were much more accurate. Also, evaluations with the aid of the scale were much more reproducible than without the scale. The scale was used to determine the relationship between infestation and damage levels caused by A. guerrerronis. The relationship between infestation and damage fitted by the equation 1ny = 4.948 - 0.121x + 1.789 1nx (R 2 = 99.87%, P < 0.0001). Therefore, these findings show that it is possible to estimate A. guerreronis population density on infested coconut fruits by using the diagrammatic scale. (author)

  3. First report of Raoiella indica Hirst (Acari: Tenuipalpidae) in South America

    International Nuclear Information System (INIS)

    Vasquez, Carlos; Quiros de G, Magally; Aponte, Orlando; Sandoval, D. Maria F.

    2008-01-01

    The presence of the red palm mite, Raoiella indica Hirst is recorded for the first time in South America. High populations and severe damages caused by this new invasive mite were found on coconut and banana leaves in Sucre (10 deg 27' 47 N and 64 deg 10' 38 W ) and Monagas (9 deg 46'60 N and 63 deg 12'0 W ) states in northeastern Venezuela. (author)

  4. [Dynamics of infection of Fringilla coelebs chaffinch nestlings with feather mites (Acari: Analgoidea)].

    Science.gov (United States)

    Mironov, S V; Malyshev, L L

    2002-01-01

    A process of infecting the chaffinch nestlings Fringilla coelebs with three analgoid feather mites, Analges passerinus L., 1758, Monojoubertia microphylla (Robin, 1877), and Pteronyssoides striatus (Robin, 1977), commonly occurred on this bird species was investigated. 15 nests contained totally 65 nestlings, from 2 to 6 individuals in a brood, have been examined from the day of hatching till 11th day. Observations were held in the neighbourhood of the bird banding station "Rybachy" (Russia, Kaliningrad Province) in June of 1982. Number of mites on alive nestlings taken temporarily from their nest was counted by means of binocular lens under the magnification x12.5 and x25. The nestlings receive the mites from the chaffinch female during the night time, when the female sits together with the young birds and heats them. In the condition of this prolonged direct contact the mites migrate from the female onto the nestlings. As it was shown in our study of seasonal dynamics of mites on the chaffinch (Mironov, 2000), the chaffinch female only gives its mites to young generation and looses about three quarter of its mite micropopulation during the nesting period (June), hile in the chaffinch males the number of mites continues to increase during all summer. The infections with three feather mite species happen in the second part of the nestling's stay in the nest. The starting time of this process, its intensity, and sex and age structure of mite micropopulations on the nestlings just before their leaving the nest are different in the mite species examined. These peculiarities of feather mite species are determined by the biology of examined species, and first of all by their morphological characteristic and specialisation to different microhabitats, i.e. certain structural zones of plumage. Pteronyssoides striatus (Pteronyssidae) is rather typical mite specialised to feathers with vanes. In adult birds with completely developed plumage this species occupies the ventral surface of the big upper coverts of primary flight feathers. This species appears on the chaffinch nestlings in a significant number on 7th day. The mites occupy the basal parts of primary flight feathers represented in that moment by the rods only. They sit on practically open and smooth surface of this microhabitat, which is uncommon for them, because the vanes of the big upper coverts are not yet open and also represented by thin rods. During the period of the last 5 days (from 7 to 11th day) the mean number of mites per one nestling increases from 2.3 +/- 0.5 to 17.1 +/- 1.8 mites. Just before the day, when the nestling leave the nest, the tritonymphs absolutely predominate (82.4%) in the micropopulation of P. striatus. Analges passerinus (Analgidae) is specialised to live in the friable layer formed by numerous not-engaged thread barbles of the down feathers and basal parts of the body covert feathers. Mites have special hooks on legs used for hard attaching to the barbles and for fast moving in the friable layer of feathers. On the chaffinch nestlings, these mites appear usually on 8th day, when the rod-like body covert feathers begin to open on apices and form short brushes; however some individuals occur on the skin of nestlings even on 6th day. The mean number of mites per nestling on the 11th day reaches 16.5 +/- 1.4 individuals. The micropopulation of A. passerinus is represented on the nestlings mainly by the females (45.5%), tritonymphs (23.6%) and males (11.5%). Monojobertia microphylla (Proctophyllodidae) is a typical dweller of feathers with large vanes. Mites of this species commonly occupy the ventral surface of primary and secondary flight feathers and also respective big upper covert feathers of wings. M. microphylla appears on the nestlings in a significant number (7.1 +/- 1.2 mites) on 9th day, only when the primary flight feathers already have short vanes about 10 mm in length. In next three days the number of mites increases very fast and reaches on 11th day 60.3 +/- 5.7 mites per nestling. In the micropopulation of this species, the tritonymphs count 38.3%, and the quota of males and females is 25.3% each. The migration of this species goes most intensively, than in two other species. An analitic selection of logistic curves shows, that the increasing of mite number during the process of infection with three mite species may be most adequately described by the sigmoid curves with clearly recognizable levels of saturation, which can be theoretically reached. Indeed, the number of mite individuals being able to migrate onto the nestlings is limited by their number on a respective chaffinch female. In a contrast, the increasing of plumage indices, for instance the length of flight feathers, has almost linear character during the period of observation. The beginning of mite migration is determined by the development of respective microhabitats in the plumage of nestlings, or at least by the development of certain structure elements of plumage, where mites are able to attach for a while, before that moment, when the nestlings will develop the plumage completely and begin to fly. In three mite species examined, the process of infection was performed by older stages, namely by the imago and/or tritonymphs. This can be explained by two reasons. On the one hand, the older stages are most active in their movement, resistible and able to survive successfully on new host individuals. On the other hand, the older stage are ready for the reproduction or will be ready after one moulting. The older stages of mites can quickly create a large and self-supporting micropopulations on the birds, therefore this strategy ensures a successful subsequent existence of the parasite species. In cases, when mites (A. passerinus, M. microphylla) migrate into the respective microhabitats structurally corresponding to their normal microhabitats on adult birds, the micropopulations of these mite species include a significant or dominant quota of females and males. When the normal microhabitat is not yet formed, feather mites migrate into neighboring structure elements of plumage, where they can survive and wait for the development of normal microhabitat, to which they are well adapted. Therefore, in the case of P. striatus, its micropopulations on the chaffinch nestlings are represented mainly by the tritonymphs.

  5. First record of the family Liacaridae (Acari, Oribatida) from Vietnam, with description of two new species

    Czech Academy of Sciences Publication Activity Database

    Ermilov, S.G.; Starý, Josef

    2017-01-01

    Roč. 22, č. 4 (2017), s. 456-466 ISSN 1362-1971 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Liacarus * Xenillus * morphology * systematics * Tam Dao National Park Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 1.467, year: 2016

  6. The Effect of Spiromesifen on the Reproductive Potential of Tetranychus urticae Koch (Acari: Tetranychidae

    Directory of Open Access Journals (Sweden)

    Dejan Marčić

    2009-01-01

    Full Text Available Laboratory bioassays were conducted to evaluate the effects of spiromesifen on the fecundity, fertility and population growth of two-spotted spider mite (Tetranychus urticae Koch after treatment of pre-ovipositing females with five acaricide concentrations: 180mg/l (maximum recommended concentration for use in glasshouses against spider mites, 18 mg/l, 1.8 mg/l and 0.18 mg/l (the last one was discriminative for eggs and immatures in preliminary studies, i.e. produced 100% mortality of those stages and 0.018 mg/l. After24h exposure, the percentages of females surviving treatment without visible symptoms of poisoning were 50% (180 mg/l, 45% (18 mg/l, 51% (1.8 mg/l, 74% (0.18 mg/l, 96% (0.018 mg/l and 98% (0 mg/l. Over the first four days after treatment, the females that survived180 mg/l and 18 mg/l laid no eggs. The total number of eggs laid after treatment with these two concentrations was reduced to less than 2% against the control by the end of the trial. The females that survived 1.8 mg/l laid 50% less eggs, compared to the control, while the number of eggs laid by the females treated with 0.18 mg/l and 0.018 mg/l were 19% and 4% lower, respectively. Over the initial four days, egg hatch rates in treatments were 73-87%, and 92-93% in the control. Significant statistical differences between gross fecundity (FCg and gross fertility (FTg values in the control and treatments were detected for females surviving 180 mg/l, 18 mg/l and 1.8 mg/l. On the other hand, only the net fertility (FTn value of females treated with 0.018 mg/l showed no statistically significant difference from the control value. Treatments with 180 mg/l and 18 mg/l significantly reduced the instantaneous rate of increase (ri 6, 8 and 10 days after treatment, compared to the control. The negative ri values in those treatments indicated a declining population. Sublethal effects of spiromesifen and its impact on T. urticae management are discussed.

  7. Fundamental aspects of genetic control of the two-spotted spider mite Tetranychus urticae (Acari: Tetranychidae)

    International Nuclear Information System (INIS)

    Feldmann, A.M.

    1979-01-01

    Radiobiological properties of Tetranychus urticae and other topics of genetic control have been evaluated. The induction by X-rays or fast neutrons of dominant lethals in mature sperm and of dominant lethals and recessive lethals in prophase-1 oocytes and the induction by both radiation types of chromosome mutations, recessive lethals and factors causing F 1 -infertility in sperm and oocytes, have been studied. From the results the optimal dose, radiation type and germ cell type could be chosen for obtaining either fully sterile males or substerile males, producing fully infertile F 1 -females. Also the most favourable conditions were determined for the induction of chromosome mutations with the lowest frequency of linked recessive lethals. The radiobiological properties of holokinetic chromosomes are extensively discussed. The successful displacement of the standard karyotype by a radiation arranged karyotype is presented and discussed in its relevance for practical application. (Auth./C.F.)

  8. Rhipicephalus sanguineus (Acari: Ixodidae) female ticks exposed to castor oil (Ricinus communis): an ultrastructural overview.

    Science.gov (United States)

    Sampieri, B R; Furquim, K C S; Nunes, P H; Camargo-Mathias, M I

    2013-02-01

    Tick control has been accomplished through the use of synthetic acaricides, which has created resistant individuals, as well as contaminating the environment and nontarget organisms. Substances of plant origin, such as oils and extracts of eucalyptus and neem leaves, have been researched as an alternative to replace the synthetic acaricides. Ricinoleic acid esters from castor oil have recently been shown as a promising alternative in eliminating bacterial contamination during ethanol fermentation, by acting as an effective biocide. The same positive results have been observed when these esters are added to the food given to tick-infested rabbits. This study tested the effect of these substance on the reproductive system of Rhipicephalus sanguineus females, added to rabbit food, more specifically on oogenesis. For this, four groups were established: four control groups (CG1, CG2, CG3, and CG4) and four treatment groups (TG1, TG2, TG3, and TG4) with one rabbit in each (New Zealand White), used as hosts. After full 4 days feeding (semi-engorgement), the females were collected and had their ovaries extracted. In this study, it was observed that R. sanguineus females exposed to esters had their ovaries modified, which was demonstrated through transmission electron microscopy techniques. The addition of ricinoleic esters to the diet of tick-infested rabbits revealed how toxic such substances are for the cytoplasmic organelles of oocytes and pedicel cells. These compounds can change the morphophysiology of germ and somatic cells, consequently influencing their viability and, therefore, confirming that the ricinoleic acid esters from castor oil are a promising substance in the control of R. sanguineus.

  9. Perspectives for mass rearing of Iphiseiodes zuluagai Denmark and Muma (Acari: Phytoseiidae)

    International Nuclear Information System (INIS)

    Albuquerque, Fabio A. de

    2008-01-01

    Iphiseiodes zuluagai Denmark and Muma is an important predator of Brevipalpus phoenicis (Geijskes) on citrus in Brazil. The suitability of Tyrophagus putrescentiae (Schrank) as a food source of I. zuluagai in laboratory rearing was investigated at 25.5 ± 0.5 deg C, 88 ± 7% RU and photophase of 12h. Initially, levels of oviposition of the predator fed on eggs were evaluated, as well as the dead or live post-embryonic stages of T. putrescentiae, in a period of 10 days. The daily oviposition rate was 1.3 egg per female when they were fed on eggs on T. putrescentiae, 0.7 egg per female when they were fed on dead post embryonic stages and about 0.3 egg per female when they were fed on live post-embryonic stages. Later, the life table of I. zuluagai was constructed, when eggs of T. putrescentiae were offered to the predators as prey. The immature stages were observed every 8 h, to determine the corresponding durations. In the adult phase, the mites were observed every 24 h, to determine the reproductive parameters. The intrinsic rate of natural increase (r m ) was 0.11 female/ female/day; resulting in a fi nite rate of increase of 1.11 (λ). The net reproductive rate (R 0 ) was 7.1 females/generation, with a mean generation time (T) 18.6 days. The results show that T. putrescentiae is a favorable food source for the development of I. zuluagai. (author)

  10. Bacteria of the genus Rickettsia in ticks (Acari: Ixodidae) collected from birds in Costa Rica

    Czech Academy of Sciences Publication Activity Database

    Ogrzewalska, M.; Literák, I.; Čapek, Miroslav; Sychra, O.; Calderón, V. Á.; Rodríguez, B. C.; Prudencio, C.; Martins, T. F.; Labruna, M. B.

    2015-01-01

    Roč. 6, č. 4 (2015), s. 478-482 ISSN 1877-959X R&D Projects: GA AV ČR IAA601690901 Institutional support: RVO:68081766 Keywords : Rickettsia * Ticks * Birds * Ixodes * Amblyomma * Costa Rica Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.690, year: 2015

  11. Canalization of body size matters for lifetime reproductive success of male predatory mites (Acari: Phytoseiidae).

    Science.gov (United States)

    Walzer, Andreas; Schausberger, Peter

    2014-04-01

    The adaptive canalization hypothesis predicts that highly fitness-relevant traits are canalized via past selection, resulting in low phenotypic plasticity and high robustness to environmental stress. Accordingly, we hypothesized that the level of phenotypic plasticity of male body size of the predatory mites Phytoseiulus persimilis (low plasticity) and Neoseiulus californicus (high plasticity) reflects the effects of body size variation on fitness, especially male lifetime reproductive success (LRS). We first generated small and standard-sized males of P. persimilis and N. californicus by rearing them to adulthood under limited and ample prey supply, respectively. Then, adult small and standard-sized males were provided with surplus virgin females throughout life to assess their mating and reproductive traits. Small male body size did not affect male longevity or the number of fertilized females but reduced male LRS of P. persimilis but not N. californicus . Proximately, the lower LRS of small than standard-sized P. persimilis males correlated with shorter mating durations, probably decreasing the amount of transferred sperm. Ultimately, we suggest that male body size is more strongly canalized in P. persimilis than N. californicus because deviation from standard body size has larger detrimental fitness effects in P. persimilis than N. californicus .

  12. An Annotated List of Tick (Acari: Ixodida) Common Names Authored by Harry Hoogstraal (1917-1986)

    Science.gov (United States)

    2011-01-01

    to the comparatively recent rules promulgated by the American Arachnological Society Committee on Common Names of Arachnids (2003), most of... Arachnological Society Committee on Common Names of Arachnids (2003) Common Names of Arachnids, Fifth Edition. American Tarantula Society, accessible online

  13. Nuevas citas de ácaros oribátidos (Acari: Oribatida para la Argentina

    Directory of Open Access Journals (Sweden)

    Ana SALAZAR MARTÍNEZ

    2006-01-01

    Full Text Available En la presente nota se citan, por primera vez para la Argentina, cuatro especies de ácaros oribátidos: Epilohmannia pallida americana Balogh & Mahunka, 1981, Protoribates (Triangius praeoccupatus (Pérez-Iñigo & Baggio, 1980, Scheloribates curvialatus Hammer, 1961 y Galumna innexa Pérez-Iñigo & Baggio, 1986. Los ejemplares fueron hallados en muestras de suelo recolectadas en La Plata, Provincia de Buenos Aires (34º 54’S, 57º 55 ́ W, en ambientes sometidos a intervención antrópica: bosques urbanos, huertas orgánicas y pastizales.

  14. Rhinoseius rafinskii, a new species from Ecuador and Venezuela (Acari, Gamasina, Ascidae)

    NARCIS (Netherlands)

    Micherdzinski, W.; Lukoschus, F.S.

    1980-01-01

    Rhinoseius rafinskii spec. nov. is described and figured in detail with all developmental stages. The species belongs to the group living in flowers and transmitted phoretically in the nasal cavities of hummingbirds in the neotropics.

  15. A new water mite of the genus Torrenticola Piersig, 1896 (Acari, Torrenticolidae from central Spain

    Directory of Open Access Journals (Sweden)

    Valdecasas, A. G.

    2005-06-01

    Full Text Available The water mite Torrenticola eureka n. sp. is described from streams of the Sierra de Guadarrama, in the center of the Iberian Peninsula. This species belongs to the subgenus Megapalpis Halbert, 1944, characterized by a long, curved rostrum and P-II longer than PIV. T. eureka n. sp. can be separated from the other species of the subgenus by the proportions of the palp segments, the size of the palp in relation to body size and the length of the cheliceral claw.El ácaro acuático Torrenticola eureka n. sp. se describe de arroyos de la Sierra de Guadarrama, en el centro de la Península Ibérica. Esta especie pertenece al subgénero Megapalpis Halbert, 1944, caracterizado por un rostro largo y curvado dorsalmente y PII más largo que PIV. T. eureka n. sp. se diferencia de las otras especies del subgénero por la combinación de los siguientes caracteres: proporción de los segmentos del palpo, tamaño del palpo en relación al cuerpo y la longitud de la uña del quelícero, entre otros caracteres.

  16. Abundance of adult ticks (Acari: Ixodidae) in the Chernobyl nuclear power plant exclusion zone.

    Science.gov (United States)

    Movila, A; Deriabina, T; Morozov, A; Sitnicova, N; Toderas, I; Uspenskaia, I; Alekhnovici, A

    2012-08-01

    The Chernobyl nuclear disaster resulted in contamination of vast areas in Europe. To date, there is little knowledge about the effects of radioactive contamination on tick species. We sampled ticks from vegetation and large-sized wild mammals belonging to orders Carnivora and Artiodactyla at sites with 0.76, 1.91, and 4.50 mSv/hr ionizing radiation background values in the Polesky State Radio-Ecological Reserve of the Chernobyl nuclear disaster zone in spring 2010. Altogether, 122 questing ticks were collected from vegetation. Among collected ticks, Dermacentor reticulatus (Fabricius) was, by far, the most abundant species (99.2%), followed by Ixodes ricnus (L.) (0.8%), which was collected only at the 0.76 mSv/hr site. The average sex ratio female∶male was 2.9∶1.0. In parallel with the present study, we examined 3 Sus scrofa (L.), 2 Nyctereutes procyonoides (Gray), and 1 Alces alces (L.) at the 4.50 mSv/hr site; 96 D. reticulatus ticks were found on 2 N. procyonoides specimens. The mean density and the intensity of infestation were 16 ticks per animal and 48 ticks per infested animal, respectively. Future investigations are warranted to further characterize the role of various tick vectors, vertebrate reservoirs, and diversity of tick-borne pathogens in the Chernobyl exclusion zone.

  17. Raillietia caprae (Acari: Raillietidae and Psoroptes ovis (Acari: Psoroptidae in the ears of goats in the state of Rio de Janeiro, Southeast Brazil Raillietia caprae (Acari: Raillietidae e Psoroptes ovis (Acari: Psoroptidae nos condutos auditivos de caprinos no Estado do Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    João Luiz H. Faccini

    2008-03-01

    Full Text Available Ear cannals of 145 domestic goats including the breeds Saanen, Toggenburg, Anglobian, Alpine, Moxoto (native and crossbred goats from 10 smallholder farms were examined by flushing for the presence of ear mites. Prevalence of Raillietia caprae Quintero, Bassols and Acevedo, 1980 was much more higher than Psoroptes ovis (Hering, 1838 in the studied area, respectively 62% ( 90/145 and 4% ( 6/145. The youngest animal parasitized was eight months old and the oldest was 10 years old. Subclinical otitis is a common feature of infestation by both species but increase of wax and the presence of pus were detected in the flushed material in approximately 10% of the goats examined.Os condutos auditivos de 145 caprinos das raças Saanen, Toggenburg, Anglobian, Alpine, Moxoto (native e mestiços provenientes de 10 pequenos criadores foram examinados pela técnica de lavagem para diagnosticar a infestação por ácaros. A prevalência de Raillietia caprae Quintero, Bassols and Acevedo, 1980 foi muito mais alta do que Psoroptes ovis (Hering, 1838 - 62% (90/145 e 4% (6/145, respectivamente. O animal parasitado mais jovem tinha oito meses de idade e o mais velho 10 anos. Otite subclínica é comum nas infestações por ambas espécies mas aumento de cerume e presença de pus foram diagnosticados no material da lavagem em aproximadamente 10% dos caprinos examinados.

  18. A review of Hyalomma scupense (Acari, Ixodidae in the Maghreb region: from biology to control

    Directory of Open Access Journals (Sweden)

    2014-01-01

    Full Text Available Hyalomma scupense (syn. Hyalomma detritum is a two-host domestic endophilic tick of cattle and secondarily other ungulates in the Maghreb region (Africa. This species transmits several pathogens, among which two are major livestock diseases: Theileria annulata and Theileria equi. Various other pathogens are also transmitted by this tick species, such as Anaplasma phagocytophilum and Ehrlichia bovis. Hyalomma scupense is common in sub-humid and semi-arid areas of several regions in the world, mainly in the Maghreb region. In this region, adults attach to animals during the summer season; larvae and nymphs attach to their hosts during autumn, but there is a regional difference in H. scupense phenology. There is an overlap between immature and adult ticks, leading in some contexts to a dramatic modification of the epidemiology of tick-borne diseases. This tick species attaches preferentially to the posterior udder quarters and thighs. Tick burdens can reach 130 ticks per animal, with a mean of 60 ticks. Calves are 70 times less infested than adult cattle. The control can be implemented through six options: (i rehabilitation of the farm buildings by roughcasting and smoothing the outer and inner surfaces of the enclosures and walls. This control option should be recommended to be combined with a thorough cleaning of the farm and its surrounding area. With regard to Theileria annulata infection, this control option is the most beneficial. (ii Acaricide application to animals during the summer season, targeting adults. (iii Acaricide application during the autumn period for the control of the immature stages. (iv Acaricide application to the walls: many field veterinarians have suggested this option but it is only partially efficient since nymphs enter deep into the cracks and crevices. It should be used if there is a very high tick burden or if there is a high risk of tick-borne diseases. (v Manual tick removal: this method is not efficient since the ticks can feed on several other animal species in the farm. This control option can lead to a reduction of the tick population, but not a decrease in tick-borne disease incidence. (vi Vaccination: this control option consists of injecting the protein Hd86; trials have shown a partial effect on nymphs, with no effect on adult ticks. Combination of two of these control options is recommended in regions where there are high burdens of important tick vectors. Further studies are needed to improve our knowledge on this tick species in the Maghreb region, since the number of published studies on Hyalomma scupense in this region is very limited.

  19. Olfactoryresponse of the predatory mite Typhlodromus pyri (Acari: Phytoseiidae) to methyl salicylate in laboratory bioassays

    Science.gov (United States)

    The response of Typhlodromus pyri, a key predator of grapevine rust mite (Calepitrimerus vitis), to MeSA was tested using a Y-tube olfactometer in laboratory bioassays. Six doses ranging from 200 to 0.002 µg of diluted MeSA were tested. Significantly higher proportions of T. pyri preferred MeSA at ...

  20. Irradiation as a phytosanitary treatment for mites of the specie Tyrophagus putrescentiae (Acari: Acaridae)

    Energy Technology Data Exchange (ETDEWEB)

    Arthur, Valter, E-mail: varthur@cena.usp.b [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil). Lab. de Radiobiologia e Ambiente; Mineiro, Jeferson L.C. [Instituto Biologico de Sao Paulo/APTA, Sao Paulo, SP (Brazil). Lab. de Entomologia Economico

    2009-07-01

    In great populations mites of the specie Tyrophagus putrescentiae can cause damages in stored products. The work had as objective to evaluate the effects of the gamma radiation of the Cobalt-60 to control the mites of the specie T. putrescentiae. The mites were irradiated with doses of 0 (control), 300, 400, 500, 600, 700, 800 Gy, in a source of Cobalt-60 type Gammacell-220, with a dose rate of 0.718 kGy/hour. Each treatment consisted of four repetitions containing 10 mites each, in a total of approximately 40 mites for treatment. The evaluations were daily, being counted the number of mites died, put eggs and emerged larvae. Based on the obtained results it was concluded that the dose sterilizing for the mites of this specie was of 300 Gy. Already the dose of 600 Gy induced the total mortality of the mites after 11 days of the irradiation process. (author)

  1. The First Report of Eustigmaeus Johnstoni (Acari: Stigmaeidae Parasitic Mite of Phlebotominae Sand Flies from Iran

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    Mehdi Badakhshan

    2013-03-01

    Full Text Available Background: Stigmaeids mites have been recorded only on Phlebotominae sand flies up to now. Five species of Eustigmaeus, and three of Stigmaeus were reported on infested sandflies in different country up to the present.Methods: Sand flies collection was done using CDC light trap and sticky paper. The mites were isolated from in­fested specimens, mounted in Puri’s medium and identified using reliable keys.Results: A mite infested Phlebotomus papatasi was observed during a study on sandflies of one of the southern provinces of Iran, near to the Persian Gulf. Several scars resulting from mite attachment were found on abdominal tergites of this female sand fly. The mites were identified as Eustigmaeus johnstoni.Conclusion: This parasitic mite is one of the eyeless species, which has a great distribution over the world, reported from Tunisia, Saudi Arabia, Pakistan, Yemen, Cyprus and Palestine. But, this is the first record of this species from Iran.

  2. Fatores de risco, aspectos clínicos e laboratoriais da asma em crianças Risk factors, clinical and laboratory aspects of asthma in children

    Directory of Open Access Journals (Sweden)

    Líllian S.L. Moraes

    2001-12-01

    hypersensitivity skin tests were performed with puncture for the detection of the following allergens: house dust mite, animals, molds, and cockroaches with positive (histamine and negative controls (physiologic solution. A logistic regression model was used to calculate the odds ratio (OR and 95% confidence intervals (95%CI adjusted for risk factors and for confounding factors. RESULTS: Among the risk factors studied, sex, parents' low level of education, low income, length of the breast feeding period, and passive smoking were not associated with the presence of asthma. The domestic exposure to allergens was similar in both groups except for the higher frequency of pets at the homes of control patients (c²=16.9; P < 0.05. Paternal history of rhinitis was the only association with asthma (OR=3.33; 95%CI: 1.03-11.17; P < 0.05. The asthmatic children presented higher frequency of positive reactions to skin tests than the controls, mainly to house dust mites: Dermatophagoides pteronyssinus (69.5%, Dermatophagoides farinae (59.3% and Blomia tropicalis (59.3%; cockroaches: Periplaneta americana (59.3%, and cat: Felis domesticus (37.3%, with OR between 11.2-21.0; P < 0.05. Eosinophilia and serum levels of total IgE were more elevated in the group of asthmatic children (P < 0.05. The positivity of the specific IgE test for Dermatophagoides pteronyssinus and Blomia tropicalis was higher in the cases than in the controls (P < 0.05. The multivariate analysis showed that sensitization to the allergens produced by cockroaches (OR=9.26; 95%CI: 2.59-33.4, animals (OR=3.93; 95%CI: 1.05-14.67 and house dust mites (OR=3.74; 95%CI: 1.18-11.8 were the most important risk factors for asthma. CONCLUSIONS: The sensitization to indoor allergens, mainly to house dust mites, cockroaches, and cats showed a strong association with asthma in this study.

  3. [Analysis of clinical features and allergic status of asthmatic patients with positive serum mycosis-specific IgE].

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    Mou, Yan; Ye, Ling; Gong, Ying; Zhang, Zhi-feng; Jin, Mei-ling

    2013-08-01

    To improve understanding of the clinical characteristics and diagnosis of allergic bronchopulmonary mycosis (ABPM). We retrospectively analyzed the clinical data, including clinical symptoms, laboratory tests, pulmonary function tests and chest CT imaging of 95 asthmatic patients with positive serum mycosis-specific IgE from January 2010 to September 2012 in Zhongshan Hospital Affiliated to Fudan University. Of the 95 patients, 59 cases met the diagnostic criteria of ABPM. There were 34 males and 25 females, with a mean age of (53 ± 4) years and a duration of asthma for (21 ± 4) years. Thirty-six cases showed mycosis hypersensitivity (MH). There were 10 males and 26 females, with a mean age of (46 ± 6) years and a duration of asthma for (16 ± 5) years. Clinical symptoms such as wheeze (52 vs 21, χ(2) = 11.159, P = 0.001), cough (54 vs 27, χ(2) = 4.859, P = 0.030) and expectoration (43 vs 9, χ(2) = 25.731, P = 0.000) were more common in the ABPM group compared to the MH group.In the ABPM group, 58 were A. fumigatus-specific IgE antibody positive, 34 Penicillium-specific IgE antibody positive and 1 only Penicillium-specific IgE antibody positive.While in the MH group, 15 were A.fumigatus-specific IgE antibody positive, 24 Penicillium-specific IgE antibody positive and 17 only Penicillium-specific IgE antibody positive.In the ABPM group, the percentage of positive fumigatus-specific IgE antibody was higher (58 vs 15, χ(2) = 24.500, P = 0.000), while the percentages of dermatophagoides pteronyssinus(21 vs 20, χ(2) = 3.632, P = 0.045) and Dermatophagoides farinae(17 vs 21, χ(2) = 8.118, P = 0.004) were lower. Total serum IgE [(4395 ± 1437) IU/ml vs (276 ± 133) IU/ml, T = 4.384, P = 0.000], peripheral eosinophil percentage[(12.56 ± 1.20)% vs (1.30 ± 0.15)%, t = 8.175, P = 0.000] and count [(2.09 ± 0.43)×10(9)/L vs (0.19 ± 0.04)×10(9)/L, t = 7.032, P = 0.000] were higher in the ABPM group as compared to the MH group.FEV1% slightly declined in the ABPM

  4. PREVALENCIA DE SENSIBILIZACIÓN EN PACIENTES PEDIÁTRICOS CON SOSPECHA O DIAGNÓSTICO DE ENFERMEDAD ALÉRGICA. ESTUDIO PRESPPENAL(1

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    María Claudia Ortega López

    2014-10-01

    Full Text Available Introducción. Las enfermedades alérgicas vienen en aumento en los últimos años tanto en prevalencia como en morbilidad y mortalidad. Se conoce que la sensibilización a uno o varios alérgenos es un factor de riesgo importante para enfermedad alérgica; sin embargo, cuantificar cómo el hecho de estar sensibilizado determina los síntomas clínicos para determinada enfermedad y rango de edad aún es motivo de controversia. Metodología: Presentamos los datos de los resultados de las pruebas positivas de alergia o pruebas de puntura realizadas a 671 de un total de 798 pacientes pediátricos de 1 a 18 años de edad con sospecha o diagnóstico clínico de enfermedad alérgica, con el fin de obtener la prevalencia de la sensibilización para aeroalérgenos y alérgenos alimentarios en tres hospitales de la ciudad de Bogotá; ciudad tropical ubicada a 2.600 metros de altura sobre el nivel del mar, con temperatura de 15°C y humedad relativa en promedio anual de 75%. Resultados: La edad promedio fue de 9.9 años, 369 (55% fueron de sexo masculino. De los aeroalérgenos, el más prevalente fue el Dermatophagoides pteronyssinus, en 493 pacientes (73.5% (IC 95% 70.1-76.8. De los alérgenos alimentarios se encontró resultado positivo en mayor proporción a la yema de huevo en el 4.9%, (IC 95% 3.2-6.3. De las patologías reportadas, la más prevalente fue la rinitis alérgica en 319 (47.5% de los pacientes. Conclusiones: En la población estudiada con patología alérgica definida por diagnóstico clínico se encontró una mayor sensibilización a aeroalérgenos por ácaros del polvo domiciliario. La patología más prevalente asociada a sensibilización con aeroalérgenos fue la rinitis alérgica seguida de asma y dermatitis atópica; en nuestro medio, la sensibilización a pólenes es relativamente baja y por alérgenos alimentarios es muy baja.

  5. The presence of serum anti-Ascaris lumbricoides IgE antibodies and of Trichuris trichiura infection are risk factors for wheezing and/or atopy in preschool-aged Brazilian children

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    Alcântara-Neves Neuza M

    2010-08-01

    Full Text Available Abstract Background The elucidation of factors that trigger the development of transient wheezing in early childhood may be an important step toward understanding the pathogenesis of asthma and other allergic diseases later in life. Transient wheezing has been mainly attributed to viral infections, although sensitisation to aeroallergens and food allergens may occur at an early age. In developing countries, intestinal helminthic infections have also been associated with allergy or atopy-related disorders. Objective The aim of this study was to explore the association of Trichuris trichiura and Ascaris lumbricoides infections with wheezing and atopy in early childhood. Study design A cross-sectional study using a Portuguese-language ISAAC phase I questionnaire, adapted for preschool-aged children, nested in a cohort study of childhood diarrhoea, was conducted on 682 children. Two faecal samples per child were examined for the presence of intestinal helminthic infection. IgE antibodies against three allergenic preparations (Dermatophagoides pteronyssinus, Blomia tropicalis and common child food, as well as against A. lumbricoides antigens, were measured in a sub-sample of these children, whose parents allowed the procedure. Atopy was defined by the presence of levels of serum IgE antibodies ≥0.35 kU/L against at least one of the three tested allergenic preparations. Results Active T. trichiura infection but not A. lumbricoides infection was positively associated with wheezing in the total studied children population [adjusted OR = 2.60; CI = 1.54;4.38] and in the atopic children sub-population [adjusted OR = 3.07; CI = 1.00;9.43]. The association with atopy was also positive and statistically significant only in the brute analysis [OR = 2.13; CI = 1.03;4.40]. Anti-A. lumbricoides IgE antibodies, but not current A. lumbricoides infection, were positively associated with wheezing in atopic children [adjusted OR = 2.01; CI = 1.00;4.50] and in non

  6. [Immunologic response to microorganisms from a polluted ventilation system in a working environment].

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    Cernelc, S; Vozelj, M

    1991-01-01

    Authors point out the morbidity of employees working in ventilation systems contaminated with various microorganisms. They analysed 96 workers exposed to air conditioning system (Group A), and 71 workers (Group B) breathing normal ambient air. The workers of both groups were subjected clinically by functionally and immunologically. Preparation of antigens "MMM" (Monday morning miseries) was used as an original method by Ajello et al. for producing antigens from systemic mycotic agents and subsequently modified. The aim of the present study is to evaluate the possibility of using ELISA in clinical practice for respiratory allergy diagnosis, and especially Hypersensitivity pneumonitis. Atopic status was determined by skin prick tests with common airborne allergens including Dermatophagoides pteronyssinus, ragweed, grasses and Aspergillus fumigatus., by Enzygnost--IgE (Behringwerke AG, Marburg) and for specific IgE by RAST technique (Pharmacia, Uppsala). The skin prick tests were performed with "MMM"-antigens. PEFR (Peak Expiratory Flow-Rate) was measured by using a Wright's peak flow meter. PEFR was recorded on Monday (first day at work) and Friday (the end of the working week). Measured values of PEFR in both groups of employees from Monday to Friday were elaborated by the Wilcoxon test. Culture of scrapings from air conditioning vents were obtained and water from the humidifier system also cultured. They were grown: T. vulgaris, Aspergillus fumigatus, Thermoactinomyces vulgaris and others. Results of questionnaires, clinical evaluation and diagnostical procedures in employees of Group A and B are as follows: Thirty eight workers in Group A had a positive clinical history of "Monday illness". In the symptomatic Group A we found in 8 cases abnormal chest roentgenogram. Further, there was no correlation between the presence of antibodies (ELISA) against MMM and pulmonary function abnormalities, as measured by either spirometry or DLCO. Further, we found good agreement

  7. Japanese Cedar (Cryptomeria japonica) Pollinosis in Jeju, Korea: Is It Increasing?

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    Lee, Jaechun; Lee, Keun Hwa; Lee, Hye Sook; Hong, Sung Chul; Kim, Jeong Hong

    2015-05-01

    Jeju is an island in South Korea located in a temperate climate zone. The Japanese cedar tree (JC) has become the dominant tree species while used widely to provide a windbreak for the tangerine orchard industry. An increase in pollen counts precedes atopic sensitization to pollen and pollinosis, but JC pollinosis in Jeju has never been studied. We investigated JC pollen counts, sensitization to JC pollen, and JC pollinosis. Participants were recruited among schoolchildren residing in Jeju City, the northern region (NR) and Seogwipo City, the southern region (SR) of the island. The JC pollen counts were monitored. Sensitization rates to common aeroallergens were evaluated by skin prick tests. Symptoms of pollinosis were surveyed. Among 1,225 schoolchildren (49.6% boys, median age 13 years), 566 (46.2%) were atopic. The rate of sensitization to Dermatophagoides pteronyssinus (35.8%) was highest, followed by D. farinae (26.2%), and JC pollen (17.6%). In the SR, 156 children (23.8%) were sensitized to JC pollen; this rate was significantly higher than that in the NR (59 children, 10.4%, P<0.001). A significant increment in the sensitization rate for JC pollen with increasing school level was observed only in the SR. JC pollen season in the SR started earlier and lasted longer than that in the NR. JC pollen season in Jeju was defined as extending from late January to mid-April. The prevalence of JC pollinosis was estimated to be 8.5%. The prevalence differed significantly between the NR and SR (5.3% vs 11.3%, P<0.001), mainly due to the difference in sensitization rates. JC pollen is the major outdoor allergen for early spring pollinosis in Jeju. JC pollen season is from late January to mid-April. Warmer weather during the flowering season scatters more JC pollen in the atmosphere, resulting in a higher sensitization rate in atopic individuals and, consequently, making JC pollinosis more prevalent.

  8. Immunophenotyping of the cutaneous cellular infiltrate after atopy patch testing in cats with atopic dermatitis.

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    Roosje, P J; Thepen, T; Rutten, V P M G; van den Brom, W E; Bruijnzeel-Koomen, C A F M; Willemse, T

    2004-10-01

    Cats with spontaneously occurring atopic dermatitis have clinical and immunocytochemical characteristics compatible with these in humans with atopic dermatitis (AD). The atopy patch test (APT) has proven to be a valuable tool in elucidating the disease process in humans. Additionally, the APT is very specific and bypasses the problem of conflicting results due to differences in chronicity of lesions of AD patients. We adapted the APT for use in cats to explore the suitability of the APT as a tool to study the onset of allergic inflammation in cats with atopic dermatitis. APT were performed in AD cats (n = 6) and healthy cats (n = 10). All cats were patch tested with two allergens in three different dilutions and a diluent control. The allergens for the APT were selected from positive intradermal test and /or prick test results and consisted of: Dermatophagoides farinae, D. pteronyssinus, Tyrophagus putrescentiae, and a grass pollen mixture. APT were read after 10, 24 and 48 h, and punch biopsies for immunohistochemical evaluation were collected at these time points. Macroscopically positive APT reactions were observed in three out of six cats at 24 and/or 48 h with allergen concentrations of 25,000 and 100,000 NU/ml. Reactions were not observed at negative control sites and neither in control animals. A significantly increased number of IL-4+, CD4+, CD3+, MHC class II+ and CD1a+ cells was found in one AD cat with positive APT reactions. Five out of six AD cats had significantly increased IL-4+ T cell numbers at 24 and/or 48 h. Our data indicate that in cats, macroscopically positive patch test reactions can be induced, which have a cellular infiltrate similar to that in lesional skin. We found a high specificity and a macroscopically positive APT reaction in half of the cats, which is similar to what is seen in humans. Hence, the APT in cats might be a useful tool in studying the immunopathogenesis of feline atopic dermatitis.

  9. [The role of the basophil activation test (BAT) in qualification for specific immunotherapy with inhalant allergens].

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    Bulanda, Małgorzata; Dyga, Wojciech; Rusinek, Barbara; Czarnobilska, Ewa

    Qualification for specific immunotherapy (SIT) according to the guidelines of the European Academy of Allergy and Clinical Immunology (EAACI) includes medical history, skin prik tests (SPT) and/or measuring the concentration of sIgE. It is necessary to perform additional diagnostic tests in case of discrepancies between the history and the results of SPT/sIgE or differences between SPT and sIgE. Basophil activation test (BAT) assesses the expression of activation markers of these cells, eg. CD63 and CD203c after stimulation. The aim of our study was to evaluate the usefulness of BAT in the qualification for the SIT in comparison to the SPT and sIgE and in case of discrepancies between the results of SPT and sIgE. The study included 30 patients with allergic rhinitis (AR) caused by allergy to house dust mite (Dermatophagoides pteronyssinus, Dp) or birch pollen qualified for SIT. All patients had SPT, sIgE and BAT determination. The group of patients with allergy to birch was a control group for Dp allergic and vice versa. BAT with CD63 antigen expression was performed using a Flow2CAST test. Basophils were stimulated with allergen preparation (50, 500, and 5000 SBU/ml concentrations). BAT results were expressed as a stimulation index (SI). For optimal concentrations of 50 and 500 SBU/ml parameters comparing BAT to SPT and sIgE as the gold standards were consecutively: sensitivity 82-100% and 93-100%, specificity 50-94% and 47-89%, positive predictive value 65- 94% and 61-87%, negative predictive value 86-100% and 93-100%. Correlation BAT - SPT and BAT - sIgE ranged within 0.59 to 0.84 and 0.51 to 0.72. BAT was helpful in 2 of 30 patients with incompatible results of SPT and sIgE. Optimal concentrations for basophil stimulation are 50 and 500 SBU/ ml. BAT may be useful diagnostic tool in the qualification for the SIT in case of discrepancies between the results of SPT and sIgE.

  10. Ectopic lymphoid tissues support local immunoglobulin production in patients with chronic rhinosinusitis with nasal polyps.

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    Song, Jia; Wang, Hai; Zhang, Ya-Na; Cao, Ping-Ping; Liao, Bo; Wang, Zhe-Zheng; Shi, Li-Li; Yao, Yin; Zhai, Guan-Ting; Wang, Zhi-Chao; Liu, Li-Meng; Zeng, Ming; Lu, Xiang; Wang, Heng; Yang, Xiang-Ping; Yu, Di; Bachert, Claus; Liu, Zheng

    2018-03-01

    The contribution of ectopic lymphoid tissues (eLTs) to local immunoglobulin hyperproduction in patients with chronic rhinosinusitis with nasal polyps (CRSwNP) is unclear. We sought to explore the cellular basis, formation mechanisms, and function of eLTs in patients with CRSwNP. We graded lymphoid aggregations in sinonasal mucosa and histologically studied their structures. The expression of lymphorganogenic factors and molecules required for immunoglobulin production was measured by using real-time PCR, and their localization was analyzed by means of immunohistochemistry and immunofluorescence. The phenotype of follicular helper T cells was analyzed by performing flow cytometry. Immunoglobulin levels were quantified by using the Bio-Plex assay or ImmunoCAP system. Nasal tissue explants were challenged ex vivo with Dermatophagoides pteronyssinus group 1 (Der p 1), and the expression of Iε-Cμ and Iε-Cγ circle transcripts was detected by using seminested PCR. Increased formation of eLTs with germinal center-like structures was discovered in patients with eosinophilic (20.69%) and noneosinophilic (17.31%) CRSwNP compared with that in patients with chronic rhinosinusitis without nasal polyps (5.66%) and control subjects (3.70%). The presence of eLTs was associated with increased expression of lymphorganogenic and inflammatory chemokines and cytokines, as well as their receptors. The expression of molecules required for immunoglobulin production, generation of follicular helper T cells, and production of IgE in eosinophilic polyps and IgG and IgA in both eosinophilic and noneosinophilic polyps were predominantly upregulated in patients with eLTs. After Der p 1 challenge ex vivo, Iε-Cμ transcript was detected only in eosinophilic polyps with eLTs but not in polyps without eLTs and noneosinophilic polyps. eLTs might support local immunoglobulin production and therefore significantly contribute to the development of CRSwNP. Copyright © 2017 American Academy of

  11. Indoor mite allergens in patients with respiratory allergy living in Porto, Portugal.

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    Plácido, J L; Cuesta, C; Delgado, L; da Silva, J P; Miranda, M; Ventas, P; Vaz, M

    1996-09-01

    We investigated the levels of mite allergens (Der p 1, Der f 1, Der 2, and Lep d 1) in dust samples from the homes of 59 patients with asthma, 36 sensitized to house-dust mites (HDM) and 23 to grass pollen (controls), living in Porto, northern Portugal. The relationship between exposure and sensitization to HDM and the influence of housing conditions on mite-allergen levels were also evaluated. Der p 1 (median 9.2 micrograms/g) and Der 2 (4.6 micrograms/g) were the main allergens, while Der f 1 and Lep d 1 levels were always 2 micrograms/g and their homes contained significantly higher levels of Der p 1 (median 12.5 vs 6.4 micrograms/g; P = 0.008) and Der 2 (6.2 vs 3.0 micrograms/g; P = 0.004) when compared to the control group. A significant correlation was observed between the exposure to Der p 1 and the wheal area at skin testing with the Dermatophagoides pteronyssinus (Dp) extract (P = 0.01) as well as with serum specific IgE levels to Dp (P = 0.03). Patients with higher levels of serum specific IgE (> or = 17.5 HRU/ml) were also more frequently exposed to Der p 1 levels > or = 10 micrograms/g (P = 0.002). Old homes, presence of carpets, and signs of dampness were conditions associated with significantly higher levels of mite allergens. In conclusion, we found high levels of Der p 1 and Der 2 particularly in the homes of HDM-sensitized patients and we confirm the relationship between exposure and sensitization to HDM, assessed by both in vivo and in vitro methods. In additional to a favorable outdoor climate, we found in our region housing conditions propitious to mite growth, suggesting that specific geographic characteristics must also be taken into account for the correct planning of mite-avoidance measures.

  12. Immune response to allergens in sheep sensitized to house dust mite

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    Velden Joanne

    2008-10-01

    Full Text Available Abstract Background House dust mite (HDM allergens are a major cause of allergic asthma. Most studies using animal models of allergic asthma have used rodents sensitized with the 'un-natural' allergen ovalbumin. It has only recently been recognized that the use of animal models based on HDM provide a more relevant insight into the allergen-induced mechanisms that underpin human allergic disease. We have previously described a sheep model of human allergic asthma that uses Dermatophagoides pteronyssinus HDM. The present study extends our understanding of the immune effects of HDM and the allergens Der p 1 and Der p 2 in the sheep model of asthma. Methods Peripheral blood sera from non-sensitized (control sheep and sheep sensitized to HDM was collected to determine immunoglobulin (Ig reactivities to HDM, Der p 1 and Der p 2 by ELISA. Bronchoalveolar lavage (BAL fluid collected following allergen challenge was also assessed for the presence of HDM-specific antibodies. To examine the cellular immune response to HDM allergens, T cell proliferation and cutaneous responses were assessed in sensitized and control sheep. Results Strong HDM- and Der p 1-specific IgE, IgG1, IgG2 and IgA serum responses were observed in sensitized sheep, while detectable levels of HDM-specific IgG1 and IgA were seen in BAL fluid of allergen-challenged lungs. In contrast, minimal antibody reactivity was observed to Der p 2. Marked T cell proliferation and late phase cutaneous responses, accompanied by the recruitment of eosinophils, indicates the induction of a cellular and delayed-type hypersensitivity (DTH type II response by HDM and Der p 1 allergen, but not Der p 2. Conclusion This work characterizes the humoral and cellular immune effects of HDM extract and its major constituent allergens in sheep sensitized to HDM. The effects of allergen in HDM-sensitized sheep were detectable both locally and systemically, and probably mediated via enzymatic and immune actions of the

  13. Variações morfológicas intra e interpopulacionais de Euseius citrifolius Denmark & Muma e Euseius concordis (Chant (Acari, Phytoseiidae Intra and interpopulational morphological variations of Euseius citrifolius Denmark & Muma and Euseius concordis (Chant (Acari, Phytoseiidae

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    Aloyséia Cristina da Silva Noronha

    2002-12-01

    Full Text Available The determination of morphologic variability within and between populations of phytoseiid mites is important for the precise species identification. Euseius citrifolius Denmark & Muma, 1970 and Euseius concordis (Chant, 1959 are phytoseiids commonly found on different crops in Brazil and other South American countries. The morphologic characterization of populations preliminarily identified as E. citrifolius and E. concordis was done through examination of 10 adult females and 10 adult males of each population and of 2 to 10 adult females and males resulting from crosses between those populations. The plant substrate and collection site of each population were: E. citrijolius: Bauhinia sp. in Arroio do Meio, Rio Grande do Sul, Coffea arabica Linnaeus in Campinas, São Paulo and Terminalia catappa Linnaeus in Petrolina, Pernambuco. E. concordis: Passiflora edulis Sims. i.flavicarpa Deg. in Arroio do Meio, Manihot esculenta (Crantz in Jaguariúna, São Paulo, Hevea brasiliensis Muell. Arg. in Pontes e Lacerda, Mato Grosso, T. catappa in Petrolina and C arabica in Viçosa, Minas Gerais. A comparison of the measurements of different structures of individuals of each population and of type specimens of E. citrifolius and E. concordis confirmed the preliminary identification of the populations. Significant relationships were observed between mean setal lengths and the respective ranges within each population. Females and males of E. citrifolius from Petrolina and E. concordis from Jaguariúna had some of the setae generally shorter than those of other populations of the same species. Measurements of males resulting from heterogamic crosses indicated that E. citrifolius and E. concordis reproduce by pseudo-arrhenotoky.

  14. Ácaros predadores (Acari em plantas nativas e cultivadas do Estado do Rio Grande do Sul, Brasil Predators mites (Acari in native and cultivated plants of the State of Rio Grande do Sul, Brazil

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    Noeli Juarez Ferla

    2002-12-01

    Full Text Available This research was carried out in twenty counties of the following regions in the state of Rio Grande do Sul: Plain, Central Depression, Plateau and Coast Plain to find out the diversity of mite predators in these places. Forty-six vegetable species were sampled, thirty species of miles of the families Anystidae, Ascidae, Cheyletidae, Cunaxidae, Phyloseiidae and Stigmaeidae were mel. The Phytoseiidae were the mite that presented the greatest diversity, being present in the majority of the sample plants. Most of the Phytoseiidae that were met belong to five species of the Euseius Wainstein, 1962 genus, the second genus of this family was Iphiseiodes DeLeon, 1966, with just one species. The Stigmaeidae come up as second family in number but fewer than Phytoseiidae. In this family, the most common mite belong to the Agistemus Sumers, 1960 genus. The biggest of the mites species (13 species, was met in Morus spp. (Moraceae and Tabebuia spp. (Bignoniaceae; Phaseolus vulgaris (Papilionaceae; only one species of the mite was met in Campomanesia spp. (Myrtaceae, Phaseolus vulgaris (Papilionaceae and Rosa spp. (Rosaceae. In Alamanda spp.(Apocinaceae, Ficus spp. (Moraceae, Jacaranda mimosifolia (Bignoniaceae and Solanum spp. (Solanaceae were met mites predators. A dichotomic key is presented to separate the families, genus and species of the mites.

  15. Standardization of a rearing procedure of Tetranychus urticae Koch (Acari: Tetranychidae) on bean (Phaseolus vulgaris): plant age and harvest time; Padronizacao da criacao de Tetranychus urticae Koch (Acari: Tetranychidae) em feijoeiro (Phaseolus vulgaris): idade da planta e tempo de colheita

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    Bustos, Alexander; Cantor, Fernando; Cure, Jose R; Rodriguez, Daniel [Universidade Militar Nueva Granada, Bogota (Colombia). Facutad de Ciencias. Programa de Biologia Aplicada], e-mail: fernando.cantor@unimilitar.edu.co

    2009-09-15

    A rearing technique was standardized to produce Tetranychus urticae Koch on Phaseolus vulgaris (ICA Cerinza variety) as a prey of the predatory mite Phytoseiulus persimilis Athias-Henriot. Two assays were conducted to assess the following variables: the most suitable plant age for mite infestation, and the best time to harvest the mites and re infest the plants. In the first experiment, four, five, six, and seven-week-old plants of P. vulgaris were infested with six T. urticae per foliole. The lower plant stratum exhibited the largest number of mites regardless of plant age. However, four-week old plants had the larger average number of individuals. In the second experiment four-week-old plants were infested with 0.5 female mite/cm{sup 2} of leaf. The number of individuals per instar of T. urticae was recorded weekly. The highest mite production occurred between four and five weeks after infestation, indicating this to be the most suitable for mite harvesting and for plant reinfestation. (author)

  16. Extratos de plantas no controle de Rhipicephalus (Boophilus microplus (Canestrini, 1887 (Acari: Ixodidae em laboratório Plant extracts in control of Rhipicephalus (Boophilus microplus (Canestrini, 1887 (Acari: Ixodidae in laboratory

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    Sônia Maria Forti Broglio-Micheletti

    2009-12-01

    Full Text Available Extratos vegetais foram estudados com o objetivo de avaliar suas eficiências no controle de Rhipicephalus (Boophilus microplus (Canestrini, 1887 em laboratório. Fêmeas ingurgitadas de carrapato foram coletadas de bovinos e mantidas em placas de Petri. Foram utilizados extratos orgânicos alcoólicos 2% (peso/volume de sementes de Annona muricata L. (Annonaceae (graviola; flores de Syzygium malaccensis (L. (Myrtaceae (jambo; folhas de Cymbopogon citratus (DC. Stapf (Poaceae (capim-santo; folhas de Azadirachta indica A. Juss (Meliaceae (nim; e extrato hexânico na mesma concentração de A. indica (sementes. Os grupos-controle foram compostos por fêmeas sem tratamento e fêmeas tratadas com água destilada e esterilizada e dimetilsulfóxido (DMSO a uma concentração de 1%. O extrato de A. muricata apresentou o maior poder acaricida, com eficácia de 100%, seguido dos extratos de S. malaccensis (75 e 59,24% e A. indica (65 e 38,49%. Houve 100% de redução na eclosão das larvas quando se utilizou o extrato de sementes de A. muricata.Plant extracts were studied to evaluate its efficiency in the control of Rhipicephalus (Boophilus microplus (Canestrini, 1887 in laboratory. Engorged female ticks were collected from the cattle, kept in Petri dishes. Organic alcoholic extracts 2% (weight/volume were used: seeds of Annona muricata L. (Annonaceae (soursop; flowers of Syzygium malaccensis (L. (Myrtaceae (iamb, leaves of Cymbopogon citratus (DC. Stapf (Poaceae (holy grass, leaves of Azadirachta indica A. Juss (Meliaceae (neem and hexane extract 2% (weight/volume of A. indica (seeds. The control groups consisted of untreated females and females treated with distilled water and sterile and dimethylsulfoxide (DMSO a concentration of 1%. The extract of A. muricata (seed had the highest mortality with acaricide activity and 100% efficacy followed by extracts of S. malaccensis (75 and 59.24% and A. indica (65 and 38.49%. The seed extract of A. muricata reduced 100% larvae hatch.

  17. Biologia e tabela de vida de Tetranychus desertorum (Acari: Tetranychidae sobre folhas de feijão (Phaseolus vulgaris Biology and life table of Tetranychus desertorum (Acari: Tetranychidae on leaves of kidney bean (Phaseolus vulgaris

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    Edgar Rivero

    2009-03-01

    Full Text Available Biological aspects and life table of the red spider mite, Tetranychus desertorum Banks, 1900, were studied on leaf discs of kidney bean (Phaseolus vulgaris Linnaeus cultivar "Tacarigua" under laboratory conditions (28 ± 2ºC, 70 ± 10% R.H. and 12:12h. Our results showed that total developmental time was 6.8 days for females, with partial duration of immature stages corresponding to 3.8, 1.4, 1.0 and 0.7 for egg, larva, protonymph and deutonymph, respectively. Preoviposition, oviposition and postoviposition periods were 1.1, 8.4 and 1.3 days, respectively; and the higher mean fecundity (6.93 eggs/female/day was observed on day 4. Female mean longevity was 10 days. The life table parameters recorded were: net reproduction rate (Ro = 41.10 individuals; generation time (T = 11.15 days; intrinsic natural growth (r m = 0.144 individuals/female/day, and finite natural increase rate (λ = 1.155 individuals/female. Our findings could be a basis for further studies devoted to determine damage and control strategies for T. desertorum on kidney bean crops.

  18. Características biológicas de Boophilus microplus (acari: ixodidae a partir de infestação experimental em cão Biological characteristics of Boophilus microplus (acari: ixodidae on dog under experimental infestation

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    Marcos P. Franque

    2007-12-01

    Full Text Available Boophilus microplus (Canestrini, 1887, um parasita comum dos bovinos, tem sido observado em cães. Com objetivo de descrever características biológicas desse parasitismo, um cão foi infestado experimentalmente com 10.000 larvas de B. microplus previamente mantidas em câmara climatizada a 27 ± 1 ºC e umidade relativa superior a 80%. A média da fase parasitária foi de 24,4 ± 1,50 dias, com uma taxa de recuperação de 0,42%. Das 21 fêmeas desprendidas natural e precocemente do hospedeiro, 6 (28% ingurgitaram o suficiente (75,1 ± 30,23mg para realizar postura. O período médio de prépostura foi de 4,33 ± 1,37 dias e o período médio de postura de 9,17 ± 2,32 dias, com produção média de 18,78 ± 15,34mg de postura. O índice médio de produção de ovos observado foi de 22,38%. Estes resultados demonstraram que fêmeas de B. microplus alimentadas em cão experimentalmente infestado, completam seu ciclo biológico. A obtenção de fêmeas, capazes de realizar posturas viáveis, sugerem a possibilidade do cão atuar como hospedeiro alternativo para B. microplus, especialmente quando não há disponível outra espécie de hospedeiro preferencial.Boophilus microplus, a common parasite of cattle, has eventually reported in dogs. To describe biological features of this parasitism, one dog was experimentally infested with 10,000 larvae of B. microplus which were previously held in acclimatized camera at 27 ±1ºC and relative humidity up to 80%. The mean of parasitic phase was 24.4 ± 1.50 days, with 0.42% of recovery rate. Of 21 natural detached B. microplus females, six engorged enough (75.1 ± 30.23mg to achieve posture. The mean period of pre-posture was 4.33 ± 1.37 days and the means period of posture was 9.17 ± 2.32, producing a mean of 18.78 ± 15.34 posture weight. The mean of eggs production index observed was 22.38%. The results showed that B. microplus females fed on dogs to complete their life cycle. The females collected were able to ovoposite viable eggs suggesting that dogs can be a possible alternative hosts to B. microplus, especially when there is no other preferential host species available.

  19. First records of the ticks Amblyomma calcaratum and A. pacae (Acari: Ixodidae parasitizing mammals of Mexico Primeros registros de las garrapatas Amblyomma calcaratum y A. pacae (Acari: Ixodidae parasitando mamíferos de México

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    Carmen Guzmán-Cornejo

    2006-06-01

    Full Text Available Based on study of ticks deposited in the Colección Nacional de Ácaros, Instituto de Biología, Universidad Nacional Autónoma de México, we report the first records in Mexico for two species of Amblyomma: Amblyomma calcaratum ex Tamandua mexicana, and Amblyomma pacae ex Tapirus bairdii. These new records increase the number of species recorded for the genus Amblyomma in Mexico to 26.Basado en la revisión de garrapatas depositadas en la Colección Nacional de Ácaros, Instituto de Biología, Universidad Nacional Autónoma de México, establecemos los primeros registros en México para 2 especies del género Amblyomma: Amblyomma calcaratum ex Tamandua mexicana y Amblyomma pacae ex Tapirus bairdii. Estos nuevos registros incrementan a 26 el número de especies del género Amblyomma distribuidas en México.

  20. Toxicidade residual de alguns agrotóxicos recomendado na agricultura sobre Neoseiulus californicus (McGregor (Acari: Phytoseiidae Residual toxicity of some pesticides recommended for citrus orchards on the predaceous mite Neoseiulus californicus (McGregor (Acari: phytoseiidae

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    Marcos Zatti da Silva

    2007-04-01

    Full Text Available O objetivo do trabalho foi avaliar a toxicidade residual de alguns agrotóxicos utilizados em citros sobre Neoseiulus californicus (McGregor em condições de laboratório. O método de bioensaio adotado foi o de contato residual. Folhas de citros da variedade Pêra, acondicionadas em arenas, foram pulverizadas em torre de Potter. A toxicidade residual dos produtos foi avaliada duas horas e 1; 3; 5; 7; 10; 14 e 21 dias após a aplicação. Em cada arena, foram transferidas dez fêmeas adultas de N. californicus, juntamente com uma quantidade suficiente de Tetranychus urticae, como fonte de alimento. As avaliações de mortalidade foram realizadas 72 horas após a transferência dos ácaros para as arenas. Os agrotóxicos acrinathrin, deltamethrin, dinocap, enxofre, fenpropathrin, óxido de fenbutatin e propargite não causaram mortalidades significativas em adultos de N. californicus. Foram registradas mortalidades de 29,8; 24,0 e 34,1% para ácaros N. californicus expostos a resíduos de duas horas de idade de abamectim, azocyclotin e cyhexatin, respectivamente. Dicofol, pyridaben e chlorfenapyr causaram 100% de mortalidade aos ácaros predadores expostos aos resíduos tóxicos dos acaricidas, com duas horas de idade. Abamectin provocou mortalidade significativa por um período inferior a um dia. Resíduos dos acaricidas azocyclotin, cyhexatin, dicofol, pyridaben e chlorfenapyr provocaram mortalidades significativas por períodos de 1; 1; 10; 10 e 21dias, respectivamente. Os resultados obtidos no presente experimento servem de subsídio para a escolha adequada dos agrotóxicos a serem utilizados em pomares de citros nos quais N. californicus esteja presente ou naqueles em que o predador venha a ser liberado. Esses resultados também servem para a escolha do momento mais favorável para a liberação dos ácaros predadores dessa espécie no campo, após a aplicação de agrotóxicos nos pomares. Estudos conduzidos em condições de campo ainda são necessários para se compreender melhor o efeito desses agrotóxicos sobre o ácaro predador.This study was carried out to evaluate the residual toxicity of some pesticides used in citrus orchards, on Neoseiulus californicus (McGregor under laboratory conditions. The residual contact bioassay method was adopted. Citrus leaves of the variety "Pêra" were sprayed in a Potter tower. The products' residual toxicity was evaluated at two hours and 1, 3, 5, 7, 10, 14 and 21 days after treatment. Ten adult females of N. californicus were transferred to each arena together with an enough amount of Tetranychus urticae to feed the predator. Mortality evaluations were performed at 72 hours after transferring the predaceous mites to the arenas. The pesticides acrinathrin, deltamethrin, dinocap, sulphur, fenpropathrin, fenbutatin oxide and propargite did not cause significant mortalities to the adults of N. californicus. Abamectim, azocyclotin and cyhexatin caused mortalities of 29.8, 24.0 and 34.1%, respectively, for N. californicus adults exposed to two-hour pesticide residues. Dicofol, pyridaben and chlorfenapyr caused 100% of mortality to the predators exposed to the two-hour acaricide residues. Abamectin provoked significant mortalities for a period shorter than one day. Residues of azocyclotin, cyhexatin, dicofol, pyridaben and chlorfenapyr caused significant mortalities for periods of 1, 1, 10, 10 and 21 days, respectively. The results of this study provided basic information for choosing the adequate pesticides to be used in citrus orchards in which N. californicus is present, or in those the predator will be released. The results are also useful for the decision of the best releasing time for N. californicus in the field, after pesticide applications. Studies carried out in the field are still necessary to understand better the effect of these pesticides under the predaceous mite.

  1. Efeito do manejo de plantas daninhas sobre Neoseiulus californicus (Acari:Phytoseiidae em pomar de macieira Effect of weed management on Neoseiulus californicus (Acari:Phytoseiidae in apple orchard

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    Lino Bittencourt Monteiro

    2002-12-01

    Full Text Available Avaliou-se a influência do manejo de plantas daninhas sobre o deslocamento de ácaros tetraniquídeos (Panonychus ulmi e Tetranychus urticae e do ácaro predador Neoseiulus californicus em um pomar de macieira 'Gala', onde foi implantado o controle biológico do ácaro vermelho, P. ulmi, por meio de liberações massais de N. californicus. As parcelas tiveram as plantas daninhas manejadas de três formas: sem manejo, com roçadas manuais e com herbicidas. As populações de ácaros foram avaliadas sobre as plantas daninhas, Plantago tormentosa e Erigeron sp, e sobre as folhas de macieira. As maiores populações de N. californicus foram observadas nas parcelas onde os manejos proporcionaram desenvolvimento de plantas daninhas na linha de plantio. Na parcela manejada com herbicida, houve maior população de ácaros tetraniquídeos sobre as macieiras, provavelmente, devido ao reduzido número de N. californicus. P. tormentosa foi o hospedeiro preferencial do ácaro predador. Concluiu-se que o manejo de plantas daninhas, na linha de plantio das macieiras, assume um importante papel no equilíbrio entre as populações de ácaros.The influence of weed management on tetranychid mites (Panonychus ulmi and Tetranychus urticae and phytoseids movement was evaluated. Neoseiulus californicus populations were released in an apple orchard for biological control of tetranychid mites. Three kinds of weed management were used: no weed control at all, manual control and control with herbicide. The mites were evaluated in Plantago tormentosa, Erigeron sp, and apple tree leaves. The highest population of N. californicus was observed where weed was not controlled. Tetranychid mites populations were abundant on apple trees when herbicide was used, probably due to the low population of N. californicus. P. tormentosa was the preferred host of phytoseids. The conclusion was that weed management plays an important role in the regulation of mite species in apple orchards.

  2. CHEMICAL CONTROL OF ACARID Brevipalpus phoenicis (Geijskes, 1939 (Acari-Tenuipalpidae, TRANSMITTER OF LEPROSIS IN CITRUS CONTROLE QUÍMICO DO ÁCARO Brevipalpus phoenicis (Geijskes, 1939 (Acari-Tenuipalpidae, TRANSMISSOR DA LEPROSE EM CITROS

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    saúl Edgard Mendez Sanchez

    2007-09-01

    Full Text Available

    Leprosis of citrus is becoming a serious problem for a nascent citriculture of Goiás State, for this disease provokes defoliation and sometimes even death of young ‘Pera’ plants in forming orchard. This experiment was developed in one property with a forming orchard, located in the municipality of Leopoldo de Bulhões - Goiás. The prescribed treatments, expressed in dose per plant, of commercial product were: 1 binapacril (Acricid 40 CE, 16 ml; 2 clofentezine (Acaristop 50 SC, 2.0 ml; 3 clofentezine (Acaristop 50 SC, 3.0 ml; 4 tefluron + binapacril (Nomolt + Acricid 40 CE - 1.2 and 5.0 ml; 5 cyhexatin (Plictran 50 BR, 3.2 ml; 6 avermectin + triona (Vertirnec 18 CE + Triona B, 3.0 ml and 25 ml, respectively; 7 control. These treatments were realized in two stages. In the first, were ministered only acarid-killers; in the second, it was added foliar fertilization with formula 20-36-20, containing more Mg, S, B, Zn, Fe, Mn, Mo and Co, at dose of 10 g fertilizer/plant. Estimations were realized before and at 7, 15 and 30 days after pulverization, obtaining at 7 days the best results with treatments 3, 4, 5 and 6 with 77.95%, 77.20%, 78.11% and 80.31% of control, respectively, although it had none statistic significance. At 15 and 30 days all treatments presented results up to 75% of control. It is accentuated that in treatments 5 and 6 of the stage that received foliar fertilization the plants recovered from chlorosis with greater rapidity, when confronted with other treatments.

    A leprose dos citros está se tomando problema sério para a nascente citricultura causando desfolhamento e às vezes até a morte das plantas jovens de pomares em formação. Visando ao controle do ácaro da leprose Brevipalpus phoenicis (Geijskes, 1939, o experimento foi realizado em uma propriedade com pomar em formação da variedade ‘Pera’, situada no município de Leopoldo de Bulhões - Goiás. Os tratamentos estabelecidos, expressos em dose do produto comercial por planta, foram: 1 binapacril (Acricid 40 CE, a 16 ml; 2 clofentezine (Acaristop 50 SC, a 2,0 ml; 3 clofentezine (Acaristop 50 SC, a 3,0 ml; 4 tefluron + binapacril (Nomolt + Acrid 40 CE, a 1,2 e 5,0 ml; 5 cyhexatin (Plictran 50 BR, a 3,2 ml; 6 avermectin + triona (Vertimec 18 CE + Triona B, a 3,0 ml e 25 ml, respectivamente; 7 testemunha. Estes tratamentos foram realizados em duas etapas. Na primeira, aplicaram-se os acaricidas isoladamente; na segunda, a cada tratamento foram adicionados 10 g/planta de adubação foliar da fórmula 20-36-20, contendo ainda Mg, S, B, Zn, Fe, Mn e Co. As avaliações foram realizadas antes e aos 7, 15 e 30 dias após a pulverização. Embora sem significância estatística, houve tendência de melhores resultados com os tratamentos 3, 4, 5 e 6 aos 7 dias após a pulverização, com 77,95; 77,20; 78,11 e 80,31% de controle respectivamente. Aos 15 e 30 dias todos os tratamentos apresentaram resultados superiores a 75% de controle. Salienta-se que nos tratamentos 5 e 6 da etapa que recebeu adubação foliar as plantas recuperaram-se da clorose com maior rapidez, quando comparados com os outros.

  3. Repellent activity of DEET against Amblyomma cajennense (Acari: Ixodidae nymphs submitted to different laboratory bioassays Atividade repelente do DEET contra ninfas de Amblyomma cajennense (Acari: Ixodidae em bioensaio laboratorial

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    Sara Fernandes Soares

    2010-03-01

    Full Text Available This study was developed to evaluate the repellent activity of N,N-diethyl-3-methylbenzamide (DEET against Amblyomma cajennense nymphs. Two repellent bioassays were compared and the effective concentration and repellent time were calculated. The fingertip test was accomplished to evaluate in vivo four concentrations of the compound (0.200; 0.100; 0.050 and 0.025 mg.cm-2 and the filter-paper bioassay to evaluate in vitro the two highest concentrations. The compound provided repellence higher than 90% in all concentrations and at least 95% repellency in the highest concentration over 5 hours. The effective concentration against 50% of tested nymphs (EC50 was 0.006 mg.cm-2 and the EC99 was 0.036 mg.cm-2. Those concentrations were lower than the ones obtained against other tick species, denoting the effectiveness of DEET against A. cajennense. The repellency time against 50% of the ticks (RT50 was 4.8 hours and the RT90 was 2.7 hours. Both bioassays were adequate to evaluate A. cajennense repellency and provided similar results; however the in vivo test is more appropriate to estimate the effective concentration and repellency time.Este estudo foi conduzido com o objetivo de avaliar a atividade repelente do N,N-diethyl-3-methylbenzamide (DEET sobre ninfas de Amblyomma cajennense. Dois bioensaios para a avaliação de repelência foram comparados e cálculos da concentração eficaz e do tempo de repelência foram realizados. Foram empregados o bioensaio da ponta do dedo, para avaliação in vivo de quatro concentações do químico (0,200; 0,100; 0,050 e 0,025 mg.cm-2 e o bioensaio do papel filtro, para a avaliação in vitro das duas concentrações mais altas. O composto conferiu mais de 90% de repelência em todas as concentrações utilizadas e 95% de repelência por mais de cinco horas na maior concentração. A concentração do composto efetiva contra 50% das ninfas testadas (CE50 foi de 0,006 mg.cm-2 e a CE99 foi de 0,036 mg.cm-2. Estas concentrações são mais baixas do que as observadas em outras espécies de carrapatos, denotando a efetividade do princípio contra A. cajennense. O tempo de repelência de 50% dos carrapatos (TR50 foi de 4,8 horas e o TR90 de 2,7 horas. Os dois bioensaios avaliados permitiram a observação de percentuais de repelência igualmente altos e se mostraram adequados para tal avaliação, sendo que o teste in vivo é mais indicado para cálculo da concentração eficaz e da duração da repelência.

  4. Flutuação populacional e sintomas de dano por ácaros (Acari em seringueira no Estado do Mato Grosso, Brasil Population fluctuation and mite (Acari damage symptoms in rubber trees in the State of Mato Grosso, Brazil

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    Noeli Juarez Ferla

    2008-04-01

    Full Text Available Apesar da grande importância da cultura da seringueira (Hevea brasiliensis Muell. Arg. para o Brasil, pouco se conhece sobre a flutuação populacional dos ácaros nessa cultura. O objetivo deste trabalho foi estudar a flutuação populacional de ácaros na seringueira no Estado do Mato Grosso, bem como observar a evolução dos sintomas de ataque de tais pragas nessa planta. O estudo foi conduzido em campos de seringueira de Itiquira e Pontes e Lacerda, Estado do Mato Grosso, com seis clones: FX 3864, RRIM 600, IAN 873, IAN 713, PB 260 e PR 255, cujas coletas foram realizadas de agosto de 1998 a julho de 2000. Na safra de 1998/1999, a amostragem foi de 150 folhas de cada um dos estratos basal, mediano e apical, em 10 plantas ao acaso. A metodologia de coleta na safra de 1999/2000 foi alterada devido aos resultados da parcial anterior de cinco folhas do estrato mediano de cada uma das 15 plantas ao acaso. Calacarus heveae Feres foi a espécie mais freqüente em Itiquira e Phyllocoptruta serigueirae Feres em Pontes e Lacerda. A queda prematura das folhas foi observada apenas em Itiquira.Despite the importance of rubber trees (Hevea brasiliensis Muell. Arg. to Brazil, little is known about mite population fluctuation on this crop. The aim of this work was to study the fluctuation of the mite population on rubber trees in the state of Mato Grosso and to observe the evolution of the symptoms caused by their attack. This study was conducted in rubber tree fields at Itiquira and Pontes e Lacerda in the state of Mato Grosso with six clones: FX 3864, RRIM 600, IAN 873, IAN 713, PB 260 and PR 255. Samplings were conducted between August 1998 and July 2000. The 1998/1999 samples consisted of 150 leaves of each of the basal, median and apical strata of 10 plants, randomly taken. The 1999/2000 sampling methodology was changed due the results for the previous partial for five leaves of the median stratum of each of 15 plants randomly taken. Calacarus heveae Feres was the most frequent species in Itiquira and Phyllocoptruta serigueirae Feres in Pontes e Lacerda. Early leaf drop was observed only in Itiquira

  5. Mortalidade do ácaro predador Neoseiulus californicus (Acari: Phytoseiidae em testes de toxicidade residual de inseticidas e acaricidas usuais em pomáceas Mortality of predatory mite Neoseiulus californicus (Acari: Phytoseiidae in residual toxicity persistence tests

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    Marcelo Gustavo Ruiz

    2008-12-01

    Full Text Available Os ácaros fitoseídeos, especialmente Neoseiulus californicus (McGregor, são importantes agentes de controle biológico de ácaros tetraniquídeos-praga nas culturas de pomáceas no "Alto Valle del Río Negro y Neuquén", Argentina. Neste trabalho, avaliou-se a mortalidade de N. californicus quando exposto a resíduos dos inseticidas azimphos-methyl, carbaryl e cyfluthrin, e dos acaricidas cyhexatin e propargite. Os produtos foram aplicados às concentrações recomendadas em plantas de pereira. Um, três, seis e dez dias após a aplicação (DAA, folhas tratadas foram retiradas das plantas para a preparação de unidades experimentais. Cinco adultos de N. californicus, provenientes de criação-estoque, foram transferidos para cada unidade, onde pólen de taboa foi fornecido como alimento. As unidades foram mantidas a 25 ± 2 ºC, 60 ± 10% de umidade relativa e fotoperíodo de 14 h. A mortalidade do ácaro foi avaliada 24 h após o confinamento. As médias de mortalidade foram comparadas pelo teste de Dunnett, a 5% de probabilidade. A progressão do declínio do efeito dos produtos testados foi submetida à análise de regressão. Nas duas primeiras datas de avaliação, todos os produtos apresentaram valores de mortalidade significativamente diferentes da testemunha tratada com água. Seis dias após a aplicação, propargite, cyhexatin e cyfluthrin apresentaram mortalidade de aproximadamente 30%, enquanto a mortalidade nos tratamentos azimphos-methyl e carbaryl apresentou níveis estatisticamente similares aos da testemunha. Dez dias após a aplicação, a mortalidade em todos os tratamentos não diferiu significativamente da testemunha. O efeito de todos os produtos apresentou declínio progressivo ao longo do período de observação, sendo significativa a 1% de probabilidade a regressão linear negativa para os valores obtidos. Os maiores efeitos negativos sobre a sobrevivência de N. californicus corresponderam aos acaricidas testados. Azimphos-methyl foi o produto que menos afetou a sobrevivência do ácaro predador. Os inseticidas testados, usados na região do "Alto Valle del Río Negro y Neuquén" para o controle de Cydia pomonella, praga-chave das culturas de pomáceas, apresentaram baixa toxicidade sobre N. californicus.Phytoseiid mites, mainly Neoseiulus californicus (McGregor, are important biological control agents of Tetranychidae pest mites in pip fruit crops in the region known as "Alto Valle del Río Negro y Neuquén", Argentina. We assessed the mortality of N. californicus when exposed to residues of the insecticides azimphos-methyl, carbaryl and cyfluthrin, as well as the acaricides cyhexatin and propargite. Pear plants were sprayed up to dip-point with pesticides in their recommended label concentrations. One, 3, 6 and 10 days after application (DAA, leaves were collected from treated plants and used to establish experimental arenas. Five adult laboratory-reared N. californicus specimens were transferred into each arena which contained Southern cattail pollen as food source. Experimental arenas were kept at 25 ± 2 ºC, 60 ± 10% RH and a photoperiod of 14 hours. Mite mortality was assessed 24 hours after the confinement. The completely randomized design was adopted for data statistical analysis, mortality means were compared by Dunnett's test (p < 0.05. Progression of pesticide's effect decline was submitted to regression analysis. On 1 and 3 DAA mean mortality in all of the treatments was significantly different from that of the water-treated control. On the sixth DAA, propargite, cyhexatin and cyfluthrin treatments caused about 30% mortality, while mortality levels in treatments with azimphos-methyl and carbaryl were statistically similar to that of control treatment. On the tenth DAA, mortality in none of the pesticide treatments differed from that of control. All of the pesticide treatments presented progressive decline throughout the experimental period, being significant (p < 0.01 the negative linear regression obtained. Tested acaricides produced the greatest negative effects on the survival of N. californicus. Azimphos-methyl was the pesticide that least affected the survival of the predatory mite. Tested insecticides, used in the "Alto Valle del Río Negro y Neuquén" region to control codling moth, C. pomonella, key pest of pip fruit crops, showed low toxicity to N. californicus.

  6. CONTROL TEST TO MICRO ACARID Aculops lycopersici (Massee, 1937 (ACARI: ERIOPHYIDAE ATTACKING TOMATO PLANT, USING A NEW ACARID-KILLER ENSAIO DE CONTROLE AO MICROÁCARO DO TOMATEIRO Aculops lycopersici (Massee, 1937 (Acari: Eriophyidae COM UM NOVO ACARICIDA

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    Roberto Magno de Castro e Silva

    2007-09-01

    Full Text Available

    This experiment was conducted in a tomato culture area in the surroundings of the Agronomy School of UFG with the objective of evaluate the effect of a new acarid-killer named avermectin against Aculops lycopersici (Massee, 1937, the tomato plant micro acarid. The experimental delineation used was random blocks with six treatments and four replicates. Treatments utilized were: Avermectin (Vertimec 18 CE, 50, 75 and 100 ml/100 1 of water; Cyhexatin (Plictran 50 BR 100 g/100 l of water; Sulphur (Kumulus 80% PM, 400g/100 1 of water and control. Pulverizations were made by using a portable costal sprayer, applying 30 ml of solution per plant. Estimations were made at 3, 7, 10, 14 and 21 days after treatments, sampling 10 leaves at random of 10 plants pertaining to 2 central lines of each parcel and counting the number of acarids aiming an area of 0.7 cm2/leaf. The results showed that the product avermectin was superior to other acarid-killers in all estimations realized, presenting a control efficiency close to 95% at smaller dosage, until 21 days. Cybexatin and sulphur reduced its efficiency during the experiment development.

    O experimento foi conduzido numa área de plantação de tomate, nas proximidades da Escola de Agronomia da UFG, com o objetivo de avaliar o efeito de um novo acaricida denominado avermectin, contra Aculops lycopersici (Massee, 1937, o microácaro do tomateiro. O delineamento experimental foi de blocos ao acaso com seis tratamentos e quatro repetições. Os tratamentos utilizados foram: Avermectin (Vertimec 18 CE, 50, 75 e 100 ml/l00 1 de água; Cyhexatin (Plictran 50 BR. 100 g/100 1 de água; Enxofre (Kumulus 80% PM. 400 g/100 1 de água e Testemunha. As aplicações foram efetuadas com um pulverizador costal manual jacto, gastando-se 30 ml de calda por planta. As avaliações foram realizadas aos 3, 7, 10, 14 e 21 dias após a aplicação, onde amostrou-se 10 folhas ao acaso, de 10 plantas das duas linhas centrais de cada parcela e contou-se o número de ácaros visando a uma área de 0,7 cm2/folha. Os resultados obtidos mostraram que o produto avermectin foi superior aos outros acaricidas, apresentando uma eficiência de controle de aproximadamente 95%, na menor dosagem, até 21 dias. O cyhexatin e o enxofre foram reduzindo sua eficiência ao longo do desenvolvimento do ensaio.

  7. The parasitism of Ixodes luciae (Acari: Ixodidae on marsupials and rodents in Peruvian Amazon O parasitismo do Ixodes luciae (Acari: ixodidae em marsupiais e em roedores na Amazônia Peruana

    Directory of Open Access Journals (Sweden)

    María Mónica Díaz

    2009-01-01

    Full Text Available In this work the infestation with I. luciae on Didelphimorphia and Rodentia in different environments of Peruvian Amazon was studied. Didelphimorphia was represented by the family Didelphidae. Specimens belonging to Caluromys lanatus, Didelphis marsupialis, Marmosops sp.2, Metachirus nudicaudatus, Philander andersoni and Philander opossum were infested with adults I. luciae and one Micoureus sp. was infested with larvae. In Rodentia, the infestation with I. luciae nymphs was restricted to Hylaeamys perenensis, Hylaeamys yunganus and Oligoryzomys microtis, while one Oecomys bicolor (all Cricetidae was infested with larvae of this species. The few larvae were found on rodents captured in primary forest. The only significant difference (P No presente trabalho, infestações por Ixodes luciae em Didelphimorphia e Rodentia em diferentes ambientes da Amazônia peruana foram estudadas. Didelphimorphia foi representada pela família Didelphidae. Espécimes pertencentes a Caluromys lanatus, Didelphis marsupialis, Marmosops sp., Metachirus nudicaudatus, Philander andersoni e Philander opossum foram encontrados infestadas por adultos de I. luciae; um Micoureus sp. foi encontrado infestado por larvas. Em Rodentia, a infestação por ninfas de I. luciae estiveram restritas a Hylaeamys perenensis, Hylaeamys yunganus e Oligoryzomys microtis, enquanto que um Oecomys bicolor (todos Cricetidae esteve infestado por larvas de I. luciae. As poucas larvas foram encontradas em roedores capturados na floresta primária. A única diferença significante (P < 0.05 na prevalência de carrapatos adultos em Didelphimorphia foi entre P. andersoni e M. nudicaudatus (distribuição chi-quadrado. A distribuição de carrapatos adultos foi significativamente diferente em P. andersoni quando comparado com M. nudicaudatus, P. opossum e D. marsupialis (teste Kruskal-Wallis. Nenhum efeito significante do mês ou ambiente foi observado em relação à infestação por carrapato adulto em Didelphimorphia. Tanto a prevalência de infestações por ninfas, como a distribuição do carrapato indicaram que H. perenensis e H. yunganus estiveram mais propensos a estarem infestados por ninfas de I. luciae do que O. microtis. A prevalência de infestações por ninfas foi maior em florestas primária e secundária do que em áreas rurais, enquanto que a abundância foi maior em floresta secundária, quando comparada com áreas rurais (P < 0.05. O teste Kruskal-Wallis indicou diferenças (P < 0.05 para infestações por ninfas durante Dezembro, em relação a Janeiro, Março, Abril e Junho. O ciclo natural de I. luciae parece ser continuo, destacado por adultos parasitando Philander e ninfas parasitando Hylaeamys em ambientes florestais.

  8. Padrão morfológico e ciclo de vida de Eutetranychus banksi (Acari: Tetranychidae de diferentes locais e hospedeiros Morphological pattern and life cycle of Eutetranychus banksi (Acari: Tetranychidae from different localities and hosts

    Directory of Open Access Journals (Sweden)

    Viviane M. Mattos

    2009-09-01

    Full Text Available Eutetranychus banksi (McGregor, 1914 is a phytophagous, polyphagous and cosmopolitan species recorded on 23 plant families in 24 countries in North, Central and South Americas, Europe, Asia, and Africa. Specimens of E. banksi present morphological variability among individuals of the same population and also among populations from different host species, being commonly referred to as a polytypic species, despite the fact that studies based on meristic comparisons of these populations are lacking. Also, there is no data available in the literature on the biological life tables of E. banksi on different hosts. Specimens collected in Brazil, Guadeloupe, Martinique, Mexico, and Paraguay were investigated in order to recognize morphological patterns among populations from different localities and hosts. A redescription of the species is provided based on data both from literature and new observations. Also, the biological cycle on three hosts, experiments of hosts exchange and the possibility of interbreeding in individuals originating from different populations were evaluated. Significant differences in the morphology of the females of different populations were detected when comparing the different hosts. The length of the dorsal pairs of setae v2, sc2, c1, c3, e1 and h1 differentiated the specimens of the populations collected from Citrus sp. from those of Hevea brasiliensis Muell. Arg. and Pachira aquatica Aubl.. Herein we observed significant variation among the life cycle of this species on the three hosts studied. When a substrate different from the one where the mites were collected was used for the biological cycle essay, significant variation was observed in several parameters such as average oviposition rate, events of arrenothoky, and sex ratio. Interbreeding with populations collected from Citrus sinensis and P. aquatica resulted in no descendants, although mating was observed.

  9. Amblyomma rotundatum (Koch, 1844 (Acari: Ixodidae two-host life-cycle on Viperidae snakes Ciclo dioxênico em Amblyomma rotundatum (Koch, 1844 (Acari: Ixodidaeparasitando serpentes da família Viperidae

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    Daniel Sobreira Rodrigues

    2010-09-01

    Full Text Available Amblyomma rotundatum is an ixodid tick that infests ectothermic animals and reproduces exclusively by parthenogenesis. This tick has been frequently reported to infest reptiles and amphibians, under natural conditions and sometimes in captivity. It was described in Brazil and several other countries of South, Central and North America. Although many studies have reported aspects of its biology, none of them has used regularly either ophidian as hosts, or controlled temperature, humidity and luminosity for parasitic stages. The objective of this experiment was to study the life cycle of A. rotundatum feeding on Viperidae snakes under room controlled conditions at 27 ± 1 ºC temperature, 85 ± 10% relative humidity and 12:12 hours photoperiod for parasitic stages, and under B.O.D incubator conditions at 27 ± 1 ºC temperature, 85 ± 10% relative humidity and scotophase for non-parasitic stages. The total duration of the life cycle ranged from 56 to 163 days (mean of 105 days. Two-host life cycle was observed for most of the ixodid population studied.Amblyomma rotundatum é um carrapato da família Ixodidae, parasito de animais pecilotérmicos, e que se reproduz exclusivamente por partenogênese. Este carrapato é frequentemente relatado infestando répteis e anfíbios em condições naturais e, às vezes, em animais de cativeiro. Ele já foi relatado no Brasil e em vários outros países das Américas do Sul, Central e do Norte. Embora muitos estudos sobre sua biologia tenham sido publicados, nunca foram utilizados ofídios como hospedeiros e, tão pouco, foram realizados ensaios com os estádios parasitários sob condições controladas de temperatura, umidade e iluminação. O objetivo deste experimento foi estudar o ciclo biológico de A. rotundatum se alimentando em serpentes da família Viperidae sob condições ambientais controladas a 27 ± 1 ºC de temperatura, 85 ± 10% de umidade relativa do ar e 12:12 horas de fotoperíodo para estágios parasitários; assim como sob condições iguais a 27 ± 1 ºC de temperatura, 85 ± 10% de umidade relativa do ar e escotofase em estufas de germinação para estádios não parasitários. A duração total do ciclo de vida variou de 56 a 163 dias (média de 105 dias. Observou-se ciclo dioxênico para a a maior parte da população dos ixodídeos em estudo.

  10. Description of a new tick species, Ixodes collaris n. sp. (Acari: Ixodidae), from bats (Chiroptera: Hipposideridae, Rhinolophidae) in Vietnam.

    Science.gov (United States)

    Hornok, Sándor; Görföl, Tamás; Estók, Péter; Tu, Vuong Tan; Kontschán, Jenő

    2016-06-10

    In a recent study on ixodid bat ticks from Eurasia, a high genetic difference was found between Ixodes vespertilionis from Europe and Vietnam. Accordingly, it was proposed that I. vespertilionis is a species complex, with at least one additional, hitherto undescribed species. The aim of the present study was to investigate the morphology of bat ticks from Vietnam and to assess their taxonomic status in comparison with those collected in Europe. Ixodid bat ticks (two females and two nymphs) collected from the pomona leaf-nosed bat (Hipposideros pomona) (Hipposideridae) and intermediate horseshoe bat (Rhinolophus affinis) (Rhinolophidae) in Vietnam showed major morphological differences from European isolates of I. vespertilionis, including the shape of the scutum, the enclosure and shape of porose areas, the presence of a caudo-lateral collar-like ridge ventrally on the basis capituli, polytrich coxae with short setae, and grouped (non-linear) arrangement of anterior pit sensillae in Haller's organ. In this study the female and the nymph of an ixodid bat tick species from Vietnam are described for the first time. The genetic and morphological differences between I. vespertilionis Koch, 1844 and these bat ticks from Vietnam justify the status of the latter as a distinct species, Ixodes collaris Hornok n. sp.

  11. PREDATORY PHYTOSEIID MITES (Acari:Phytoseiidae) AS BIOINDICATORS OF STRESS IMPACT ON A FARMLAND AND BUTRESSES OF THE FARMLAND REVIVAL

    Czech Academy of Sciences Publication Activity Database

    Zacharda, Miloslav

    2001-01-01

    Roč. 20, č. 1 (2001), s. 47-56 ISSN 1335-342X Institutional research plan: CEZ:AV0Z6087904 Keywords : farmland * stress * revival Subject RIV: GF - Plant Pathology, Vermin, Weed, Plant Protection Impact factor: 0.192, year: 2001

  12. Thalassornectes (Alcidectes) aukletae, new subgenus and species (Acari: Hypoderatidae) from the crested and parakeet auklets (Aves: Charadriiformes; Alcidae).

    Science.gov (United States)

    Pence, D B; Hoberg, E P

    1991-03-01

    In the genus Thalassornectes, a new subgenus, Alcidectes, and a new species, T. (Alcidectes) aukletae, are described from deutonymphs in the subcutaneous adipose tissue of the crested auklet, Aethia cristatella (Pallas), and the parakeet auklet, Cyclorrhynchus psittacula (Pallas), from the eastern Pacific USSR. The new subgenus and species differ from one or both of the single species in each of the other two subgenera, Thalassornectes and Rallidectes, by (1) the normal size, position, and parallel arrangement of the genital papillae; (2) the larger size of seta sce; (3) the greater length and stronger development of setae sci, d1, l1, h, and sh; (4) the equal size of tarsi III and IV or their size subequal, with tarsus IV slightly longer than tarsus III; (5) both epimera I and sternum well developed and nearly equal in length; and (6) the free sclerotized posteriad extension from epimerite II on the ventral cuticular surface. This is the first hypoderatid reported from the host order Charadriiformes. The distribution of T. (Alcidectes) aukletae among auklets may be attributed to either cospeciation or may have an ecological basis; data are insufficient at present to sustain either hypothesis.

  13. Cattle fever tick, Rhipicephalus annulatus (Acari: Ixodidae), and the quest for discovery of its natural enemies in the Balkan Region

    Science.gov (United States)

    Cattle fever tick, Rhipicephalus annulatus (CFT), is a hard tick native to the Mediterranean region that is invasive in the southwestern USA. The tick is known to develop on cattle and white tailed deer, and it transmits two lethal diseases, piroplasmosis and babesiosis. Extensive use of acaricides...

  14. DIAGNÓSTICO TAXONÓMICO DE LA FAMILIA TENUIPALPIDAE (ACARI: TETRANYCHOIDEA EN EL VALLE DEL CAUCA (COLOMBIA

    Directory of Open Access Journals (Sweden)

    Mesa Nora Cristina

    2013-07-01

    Full Text Available Varias especies de ácaros de la familia Tenuipalpidae, especialmente las del géneroBrevipalpus Donnadieu, son reconocidas como vectores de enfermedades viralesen diferentes cultivos comerciales. La falta de estudios taxonómicos en esta familiade ácaros en Colombia y la posibilidad de introducir en forma accidental especiesde estos ácaros planos, son una amenaza para la bioseguridad de la producciónfrutícola del país. El objetivo de este trabajo es contribuir a la identificación de lasespecies de Tenuipalpidae en la vegetación silvestre y cultivada en el departamentodel Valle del Cauca y confirmar la identidad taxonómica de las especies deBrevipalpus en cítricos, guayaba, maracuyá, café y orquídeas. Las recoleccionesde ejemplares se realizaron en épocas seca y lluviosa en varios municipios deldepartamento. El montaje de los ácaros se realizó en medio Hoyer. La especiepredominante en todas las localidades y en los hospedantes recolectados fueBrevipalpus phoenicis Geijskes (1939. Por el contrario, B. obovatus Donnadieuse encontró en poblaciones muy bajas en limón Tahití y mandarina en algunaslocalidades de Palmira. Tenuipalpus anacardii De León se presentó en Anacardiumexcelsum (Bertero y Balb. ex Kunth Skeels, en el municipio de Roldanillo y T.pacificus Baker se encontró en el corregimiento de Rozo, municipio de Palmira,sobre plantas importadas de un cultivo comercial de Dendrobium sp., mientrasque Dolichotetranychus floridanus (Banks solamente se encontró sobre Ananascomosus (L. Merr., en muestras recolectadas en Cali

  15. Oribatid mite communities and metal bioaccumulation in oribatid species (Acari, Oribatida) along the heavy metal gradient in forest ecosystems

    International Nuclear Information System (INIS)

    Skubala, Piotr; Kafel, Alina

    2004-01-01

    The responses of oribatid communities to heavy metal contamination were studied. Concentration of cadmium, copper and zinc in nine oribatid species along a gradient of heavy metal pollution was measured. Oribatid mites were sampled seasonally during two years in five forests located at different distances from the zinc smelter in the Olkusz District, southern Poland. The most numerous and diverse oribatid communities were found in the forest with moderate concentrations of heavy metals. Analysis by atomic absorption spectrophotometry revealed large differences in metal body burdens among species. All studied oribatid species appeared to be accumulators of copper with Oppiella nova, Nothrus silvestris and Adoristes ovatus characterized by the highest bioaccumulation factors. Most species poorly accumulate cadmium and zinc. The accumulation of heavy metals in the body of oribatids was not strictly determined by their body size or the trophic level at which they operate

  16. Cattle Fever Tick, Rhipicephalus (Boophilus) microplus, (Acari: Ixodidae): potential control on pastures by the application of urea fertilizer

    Science.gov (United States)

    The southern cattle fever tick, Rhipicephalus (Boophilus) microplus, spends as much as 80–90% of its life cycle as a larva questing for a host. Standard control methods are limited to on-host applications, leaving a need for methods directed at the pasture infesting stages. Reports from Brazil indic...

  17. In vitro acaricidal efficacy of plant extracts from Brazilian flora and isolated substances against Rhipicephalus microplus (Acari: Ixodidae).

    Science.gov (United States)

    Valente, Paula Pimentel; Amorim, Juliana Mendes; Castilho, Rachel Oliveira; Leite, Romário Cerqueira; Ribeiro, Múcio Flávio Barbosa

    2014-01-01

    The tick Rhipicephalus microplus causes significant losses in livestock cattle and has developed increasing resistance to the primary acaricides that are used to treat these infections. The objective of this study was to identify new biomolecules or isolated substances showing acaricidal activity from plants. Larval packet tests were conducted to evaluate the effects of 11 species of plants and three isolated substances (betulinic acid, eugenol, and nerolidol) on R. microplus. An adult female immersion test was performed with the substance that showed the highest larvicidal activity, which was evaluated for inhibition of reproduction. Tests using Licania tomentosa, Hymenaea stigonocarpa, Hymenaea courbaril, Stryphnodendron obovatum, Jacaranda cuspidifolia, Jacaranda ulei, Struthanthus polyrhizus, Chrysobalanus icaco, Vernonia phosphorea, Duguetia furfuracea, and Simarouba versicolor extracts as well as the isolated substance betulinic acid indicated lower acaricidal effects on R. microplus larvae. The extract displaying the best larvicidal activity was the ethanolic extract from L. tomentosa at a concentration of 60%, resulting in a mortality rate of 40.3%. However, nerolidol and eugenol showed larvicidal activity, which was highest for eugenol. Nerolidol caused a 96.5% mortality rate in the R. microplus larvae at a high concentration of 30%, and eugenol caused 100% mortality at a concentration of 0.3%. In the adult immersion test, 5% eugenol was identified as a good biomolecule for controlling R. microplus, as demonstrated by its high acaricidal activity and inhibition of oviposition.

  18. New species of the genus Lauroppia Subías & Mínguez, 1986 (Acari, Oribatida, Oppiidae from Iran

    Directory of Open Access Journals (Sweden)

    Subías, L. S.

    2008-12-01

    Full Text Available Two new species of oribatid mites of the family Oppiidae Sellnick, 1937, Lauroppia iranica sp. nov. and Lauroppia persiangulfia sp. nov., are described from Mazandaran province, Northern Iran. These two new species are characterized by their long sensillus, with a bipectinate fusiform head and seven medium long barbs in L. iranica and a pectinate head and six long barbs in L. persiangulfia. Also an identification key to the Iranian species of Lauroppia is given.Dos nuevas especies de ácaros oribátidos de la familia Oppiidae Sellnick, 1937, Lauroppia iranica sp. nov. and Lauroppia persiangulfia sp. nov., se describen de la provincia de Mazandaran, norte de Irán. Estas dos nuevas especies se caracterizan por su sensilo largo, con una cabeza fusiforme bipectinada y con siete bárbulas de longitud media en L. iranica y con una cabeza pectinada y seis bárbulas largas en L. persiangulfia.

  19. Effect of culture media on virulence of Hirsutella thompsonii (Fischer) (Deuteromycetes) to control Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae)

    International Nuclear Information System (INIS)

    Rossi-Zalaf, Luciana S.; Alves, Sergio B.; Vieira, Solange A.

    2008-01-01

    The virulence of Hirsutella thompsonii (Fischer) to Brevipalpus phoenicis (Geijskes) was evaluated in laboratory, grown on complete and solid culture media (MC-S); complete and liquid culture media (MC-L); rice (APC) and powdered rice (APC-SM). Adults were confined to arenas prepared with citrus leaves in acrylic dishes containing water-agar. Conidial suspensions were prepared at different concentration (3.2 x 10 5 to 1 x 10 7 spores/ml) and applied on mites to establish the table curve-response on fourth day. For field evaluation, adults were maintained in arenas prepared with fruits which were placed in plants. In this test, four treatments were tried: H. thompsonii cultured on rice (APC) at two concentrations (20 kg/ha and 10 kg/ha), H. thompsonii produced by liquid fermentation (MC-L) (5 L/ha) and control (sterile water). Adult survival, number of eggs and nymphs per fruit were observed 10 and 20 days after the fungus application. The lowest LC 25 value calculated was from pathogen produced in MC-S (1.9 x 10 5 conidia/ml).The LC 25 values calculated to APC and APC-SM did not differ statistically. The LC 25 values to MC-L and MC-S were 1.9 x 10 6 infective cells/ml and 2.2 x 10 5 conidia/ml. In the fi eld, concentration and time to death differed between treatments and control. The applications resulted in reduction of adult survival and number of eggs. (author)

  20. Eriophyoid mites (Acari: Trombidiformes: Eriophyoidea) of Rosales trees in Iran: two new species and three new records.

    Science.gov (United States)

    Lotfollahi, Parisa; Irani-Nejad, Karim Haddad; De Lillo, Enrico

    2014-09-11

    This paper describes two new species of Eriophyoidea associated with trees belonging to the order Rosales in the south-western portion of East Azerbaijan province, Iran, collected during a survey in 2011: Aceria lobolinguae n. sp. on Elaeagnus angustifolia L. (Elaeagnaceae) and Rhinophytoptus nemalobos n. sp. on Prunus domestica L. (Rosaceae). Additionally, Phyllocoptes abaenus Keifer on Prunus armeniaca L. (Rosaceae), Aculus fockeui (Nalepa & Trouessart) on Prunus amygdalus Stokes and Malus domestica Borkh. (Rosaceae), and Aceria mori (Keifer) on Morus alba L. (Moraceae) were collected and are new records for the mite fauna of Iran. New locality records and host plant data are provided for Eriophyes similis (Nalepa), Eriophyes pyri (Pagenstecher) and Calepitrimerus baileyi (Keifer) which are eriophyoid species previously known from Iran. 

  1. Mites of the superfamily Rhodacaroidea (Acari: Mesostigmata) from Colombia, with a key for the world species of Desectophis Karg (Ologamasidae).

    Science.gov (United States)

    Rueda-Ramirez, Diana; Castilho, Raphael C; De Moraes, Gilberto J

    2013-11-08

    Three species of Rhodacaroidea are reported from soil in Colombia. Desectophis anthuriumsetis n. sp. (Ologamasidae) and Multidentorhodacarus colombianus n. sp. (Rhodacaridae) are described from newly-collected material. Multidentorhodacarus triramulus (Karg, 1998) is redescribed from the holotype and adult females collected in this study. A key for the separation of females of the five recognisable world species of Desectophis Karg is provided.

  2. Toxicity of Piper aduncum L. (Piperales: Piperaceae) from the Amazon forest for the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).

    Science.gov (United States)

    Silva, Wilson Castro; Martins, João Ricardo de Souza; de Souza, Hellen Emília Menezes; Heinzen, Horacio; Cesio, Maria Verônica; Mato, Mauricio; Albrecht, Francine; de Azevedo, João Lúcio; de Barros, Neiva Monteiro

    2009-10-14

    The mortality of 14-21-day-old Rhipicephalus (Boophilus) microplus larvae, and the mortality and fertility of groups of engorged adult females exposed to different concentrations of hexane, ethyl acetate and ethanol extracts of spiked pepper (Piper aduncum) were evaluated, using a completely randomized design with five treatment groups, two control groups, and two replicates for the larvae and five replicates for the adult females. Similar methodology was used to investigate the toxicity of the essential oil hydro-distillate (94.84% dillapiole) obtained from the P. aduncum crude hexane extract. The LC(50) of the hexane extract was 9.30 mg ml(-1) for larvae and the reproduction reduction ranged from 12.48% to 54.22%, while 0.1mg/ml(-1) of the essential oil induced 100% mortality in larvae. Literature reports on natural products active against R. microplus were listed and compared with the results presented here. These results indicate that P. aduncum extracts, and particularly its essential oil, are potential alternative control agents for R. microplus.

  3. Demodex lutrae n. sp. (Acari) in European otter Lutra lutra (Carnivora: Mustelidae) with data from other demodecid mites in carnivores.

    Science.gov (United States)

    Izdebska, Joanna N; Rolbiecki, Leszek

    2014-12-01

    This article describes morphological characteristics and occurrence of Demodex lutrae n. sp., which was found on European otter Lutra lutra (Linnaeus, 1758) in Poland. The new species was found in hairy regions of otter skin, mainly in the head area. With respect to morphological features, D. lutrae is most similar to D. canis (Leydig, 1859) from the domestic dog Canis familiaris Linnaeus, 1758. The new species is a medium-sized demodecid mite (adult stages average 200 μm in length); characteristic features of these mites are hammer-shaped supracoxal spines (setae elc.p) on dorsal side of gnathosoma and palps with 3 conical spines. Demodex lutrae is the first representative of the family Demodecidae described in a host from the subfamily Lutrinae. This paper also contains a checklist of demodecid mites known from carnivores.

  4. Phylogenetic relationships in Demodex mites (Acari: Demodicidae) based on mitochondrial 16S rDNA partial sequences.

    Science.gov (United States)

    Zhao, Ya-E; Wu, Li-Ping

    2012-09-01

    To confirm phylogenetic relationships in Demodex mites based on mitochondrial 16S rDNA partial sequences, mtDNA 16S partial sequences of ten isolates of three Demodex species from China were amplified, recombined, and sequenced and then analyzed with two Demodex folliculorum isolates from Spain. Lastly, genetic distance was computed, and phylogenetic tree was reconstructed. MEGA 4.0 analysis showed high sequence identity among 16S rDNA partial sequences of three Demodex species, which were 95.85 % in D. folliculorum, 98.53 % in Demodex canis, and 99.71 % in Demodex brevis. The divergence, genetic distance, and transition/transversions of the three Demodex species reached interspecies level, whereas there was no significant difference of the divergence (1.1 %), genetic distance (0.011), and transition/transversions (3/1) of the two geographic D. folliculorum isolates (Spain and China). Phylogenetic trees reveal that the three Demodex species formed three separate branches of one clade, where D. folliculorum and D. canis gathered first, and then gathered with D. brevis. The two Spain and five China D. folliculorum isolates did not form sister clades. In conclusion, 16S mtDNA are suitable for phylogenetic relationship analysis in low taxa (genus or species), but not for intraspecies determination of Demodex. The differentiation among the three Demodex species has reached interspecies level.

  5. Detection, Prevalence and Phylogenetic Relationships of Demodex spp and further Skin Prostigmata Mites (Acari, Arachnida) in Wild and Domestic Mammals.

    Science.gov (United States)

    Sastre, Natalia; Francino, Olga; Curti, Joseph N; Armenta, Tiffany C; Fraser, Devaughn L; Kelly, Rochelle M; Hunt, Erin; Silbermayr, Katja; Zewe, Christine; Sánchez, Armand; Ferrer, Lluís

    2016-01-01

    This study was conceived to detect skin mites in social mammals through real-time qPCR, and to estimate taxonomic Demodex and further Prostigmata mite relationships in different host species by comparing sequences from two genes: mitochondrial 16S rRNA and nuclear 18S rRNA. We determined the mite prevalence in the hair follicles of marmots (13%) and bats (17%). The high prevalence found in marmots and bats by sampling only one site on the body may indicate that mites are common inhabitants of their skin. Since we found three different mites (Neuchelacheles sp, Myobia sp and Penthaleus sp) in three bat species (Miotis yumanensis, Miotis californicus and Corynorhinus townsendii) and two different mites (both inferred to be members of the Prostigmata order) in one marmot species (Marmota flaviventris), we tentatively concluded that these skin mites 1) cannot be assigned to the same genus based only on a common host, and 2) seem to evolve according to the specific habitat and/or specific hair and sebaceous gland of the mammalian host. Moreover, two M. yumanensis bats harbored identical Neuchelacheles mites, indicating the possibility of interspecific cross-infection within a colony. However, some skin mites species are less restricted by host species than previously thought. Specifically, Demodex canis seems to be more transmissible across species than other skin mites. D. canis have been found mostly in dogs but also in cats and captive bats. In addition, we report the first case of D. canis infestation in a domestic ferret (Mustela putorius). All these mammalian hosts are related to human activities, and D. canis evolution may be a consequence of this relationship. The monophyletic Demodex clade showing closely related dog and human Demodex sequences also supports this likely hypothesis.

  6. Complete sequence analysis of 18S rDNA based on genomic DNA extraction from individual Demodex mites (Acari: Demodicidae).

    Science.gov (United States)

    Zhao, Ya-E; Xu, Ji-Ru; Hu, Li; Wu, Li-Ping; Wang, Zheng-Hang

    2012-05-01

    The study for the first time attempted to accomplish 18S ribosomal DNA (rDNA) complete sequence amplification and analysis for three Demodex species (Demodex folliculorum, Demodex brevis and Demodex canis) based on gDNA extraction from individual mites. The mites were treated by DNA Release Additive and Hot Start II DNA Polymerase so as to promote mite disruption and increase PCR specificity. Determination of D. folliculorum gDNA showed that the gDNA yield reached the highest at 1 mite, tending to descend with the increase of mite number. The individual mite gDNA was successfully used for 18S rDNA fragment (about 900 bp) amplification examination. The alignments of 18S rDNA complete sequences of individual mite samples and those of pooled mite samples ( ≥ 1000mites/sample) showed over 97% identities for each species, indicating that the gDNA extracted from a single individual mite was as satisfactory as that from pooled mites for PCR amplification. Further pairwise sequence analyses showed that average divergence, genetic distance, transition/transversion or phylogenetic tree could not effectively identify the three Demodex species, largely due to the differentiation in the D. canis isolates. It can be concluded that the individual Demodex mite gDNA can satisfy the molecular study of Demodex. 18S rDNA complete sequence is suitable for interfamily identification in Cheyletoidea, but whether it is suitable for intrafamily identification cannot be confirmed until the ascertainment of the types of Demodex mites parasitizing in dogs. Copyright © 2012 Elsevier Inc. All rights reserved.

  7. Detection, Prevalence and Phylogenetic Relationships of Demodex spp and further Skin Prostigmata Mites (Acari, Arachnida in Wild and Domestic Mammals.

    Directory of Open Access Journals (Sweden)

    Natalia Sastre

    Full Text Available This study was conceived to detect skin mites in social mammals through real-time qPCR, and to estimate taxonomic Demodex and further Prostigmata mite relationships in different host species by comparing sequences from two genes: mitochondrial 16S rRNA and nuclear 18S rRNA. We determined the mite prevalence in the hair follicles of marmots (13% and bats (17%. The high prevalence found in marmots and bats by sampling only one site on the body may indicate that mites are common inhabitants of their skin. Since we found three different mites (Neuchelacheles sp, Myobia sp and Penthaleus sp in three bat species (Miotis yumanensis, Miotis californicus and Corynorhinus townsendii and two different mites (both inferred to be members of the Prostigmata order in one marmot species (Marmota flaviventris, we tentatively concluded that these skin mites 1 cannot be assigned to the same genus based only on a common host, and 2 seem to evolve according to the specific habitat and/or specific hair and sebaceous gland of the mammalian host. Moreover, two M. yumanensis bats harbored identical Neuchelacheles mites, indicating the possibility of interspecific cross-infection within a colony. However, some skin mites species are less restricted by host species than previously thought. Specifically, Demodex canis seems to be more transmissible across species than other skin mites. D. canis have been found mostly in dogs but also in cats and captive bats. In addition, we report the first case of D. canis infestation in a domestic ferret (Mustela putorius. All these mammalian hosts are related to human activities, and D. canis evolution may be a consequence of this relationship. The monophyletic Demodex clade showing closely related dog and human Demodex sequences also supports this likely hypothesis.

  8. Evaluation of four commercial natural products for repellency and toxicity against the lone star tick, Amblyomma americanum (Acari: Ixodidae).

    Science.gov (United States)

    Machtinger, Erika T; Li, Andrew Y

    2017-12-01

    Lone star ticks are aggressive ectoparasites of domestic and wild animals, as well as humans. These ticks can transmit many pathogens that cause disease including Erhlichia and tularemia. Common compounds used for personal protection and area sprays are N-diethyl-3-methyl benzamide (DEET) and permethrin, but public concern over personal and environmental safety require the development of new, safer products. In the current study, four commercially available products (Wondercide, Essentria IC 3 , Vet's Best, and Mosquito Barrier) were tested for both repellent and toxic effects against lone star tick nymphs and adults. Overall, all four products were more effective against nymphs than against adults. Wondercide and Essentria IC 3 were as toxic to nymphs as permethrin at concentrations of 3.13% and higher, and as repellent as DEET at all concentrations. Nymphs were also repelled by Mosquito Barrier and Vet's Best, but these products had about half or less of the repellent effects of Wondercide and Essentria IC 3 at most of the concentrations. Adult ticks were repelled similarly by all products at all tested concentrations, but at lower levels than nymphs. Toxicity of the four tested products on adults was similar at concentrations of 12.5% and below, less than half of what was observed with permethrin with declining effectiveness as concentrations decreased. Overall, these four products may offer a natural way to repel lone star ticks, but further field testing is needed to determine rates of application and residual activity.

  9. Feeding of the panphytophagous oribatid mite Scheloribates laevigatus (Acari: Oribatida) on cyanobacterial and algal diets in laboratory experiments

    Czech Academy of Sciences Publication Activity Database

    Hubert, J.; Lukešová, Alena

    2001-01-01

    Roč. 16, - (2001), s. 77-83 ISSN 0929-1393 R&D Projects: GA ČR GA206/97/0629 Institutional research plan: CEZ:AV0Z6066911 Keywords : feeding * Oribatida * digestive tract Subject RIV: EH - Ecology, Behaviour Impact factor: 1.150, year: 2001

  10. Etoxazole resistance in predatory mite Phytoseiulus persimilis A.-H. (Acari: Phytoseiidae): Cross-resistance, inheritance and biochemical resistance mechanisms.

    Science.gov (United States)

    Yorulmaz Salman, Sibel; Aydınlı, Fatma; Ay, Recep

    2015-07-01

    Phytoseiulus persimilis of the family Phytoseiidae is an effective predatory mite species that is used to control pest mites. The LC50 and LC60 values of etoxazole were determined on P. persimilis using a leaf-disc method and spraying tower. A laboratory selection population designated ETO6 was found to have a 111.63-fold resistance to etoxazole following 6 selection cycles. This population developed low cross-resistance to spinosad, spiromesifen, acetamiprid, indoxacarb, chlorantraniliprole, milbemectin and moderate cross-resistance to deltamethrin. PBO, IBP and DEM synergised resistance 3.17-, 2.85- and 3.60-fold respectively. Crossing experiments revealed that etoxazole resistance in the ETO6 population was an intermediately dominant and polygenic. In addition, detoxifying enzyme activities were increased 2.71-fold for esterase, 3.09-fold for glutathione S-transferase (GST) and 2.76-fold for cytochrome P450 monooxygenase (P450) in the ETO6 population. Selection for etoxazole under laboratory conditions resulted in the development of etoxazole resistance in the predatory mite P. persimilis that are resistant to pesticides are considered valuable for use in resistance management programmes within integrated pest control strategies. Copyright © 2014 Elsevier Inc. All rights reserved.

  11. Sublethal effects of fenpyroximate and pyridaben on two predatory mite species, Neoseiulus womersleyi and Phytoseiulus persimilis (Acari, Phytoseiidae).

    Science.gov (United States)

    Park, Jung-Joon; Kim, Minsik; Lee, Joon-Ho; Shin, Key-Il; Lee, Sung Eun; Kim, Jeong-Gyu; Cho, Kijong

    2011-07-01

    Laboratory bioassays were conducted to evaluate the sublethal effects of fenpyroximate and pyridaben on life-table parameters of two predatory mites species, Neoseiulus (= Amblyseius) womersleyi and Phytoseiulus persimilis. In these assays, young adult females were treated with three sublethal concentrations of each acaricide. The life-table parameters were calculated at each acaricide concentration, and compared using bootstrap procedures. For each acaricide, the LC(50) estimates for both species were similar, yet the two species exhibited completely different susceptibility when the population growth rate was used as the endpoint. Exposure to both acaricides reduced the net reproduction rate (R (o)) in a concentration-dependent manner and their EC(50)s were equivalent to less than LC(7). Two different scales of population-level endpoints were estimated to compare the total effect between the species and treatments: the first endpoint values were based on the net reproductive rate (fecundity λ) and the second endpoint values incorporated the mean egg hatchability into the net reproductive rate (vitality λ). The fecundity λ decreased in a concentration-dependent manner for both acaricide treatments, but the vitality λ decreased abruptly after treatment of N. womersleyi with pyridaben. The change in the patterns of λ revealed that the acaricide effects at the population level strongly depended on the life-history characteristics of the predatory mite species and the chemical mode of action. When the total effects of the two acaricides on N. womersleyi and P. persimilis were considered, fenpyroximate was found to be the most compatible acaricide for the augmentative release of N. womersleyi after treatment.

  12. PCR-based identification of the pathogenic bacterium, Acaricomes phytoseiuli, in the biological control agent Phytoseiulus persimilis (Acari: Phytoseiidae)

    NARCIS (Netherlands)

    Gols, R.; Schütte, C.; Stouthamer, R.; Dicke, M.

    2007-01-01

    The predatory mite, Phytoseiulus persimilis is an important biological control agent of herbivorous spider mites. This species is also intensively used in the study of tritrophic effects of plant volatiles in interactions involving plants, herbivores, and their natural enemies. Recently, a novel

  13. Sex-specific developmental plasticity of generalist and specialist predatory mites (Acari: Phytoseiidae) in response to food stress.

    Science.gov (United States)

    Walzer, Andreas; Schausberger, Peter

    2011-03-01

    We studied developmental plasticity under food stress in three female-biased size dimorphic predatory mite species, Phytoseiulus persimilis, Neoseiulus californicus, and Amblyseius andersoni. All three species prey on two-spotted spider mites but differ in the degree of adaptation to this prey. Phytoseiulus persimilis is a specialized spider mite predator, N. californicus is a generalist with a preference for spider mites, and A. andersoni is a broad generalist. Immature predators were offered prey patches of varying density and their survival chances, dispersal tendencies, age and size at maturity measured. Amblyseius andersoni dispersed earlier from and had lower survival chances in low density prey patches than N. californicus and P. persimilis. Age at maturity was not affected by prey density in the generalist A. andersoni, whereas both the specialist P. persimilis and the generalist N. californicus accelerated development at low prey densities. Species-specific plasticity in age at maturity reflects opposite survival strategies when confronted with limited prey: to prematurely leave and search for other food (A. andersoni) or to stay and accelerate development (P. persimilis, N. californicus). In all species, size at maturity was more plastic in females than males, indicating that males incur higher fitness costs from deviations from optimal body size.

  14. New approach for the study of mite reproduction: The first transcriptome analysis of a mite, Phytoseiulus persimilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Cabrera, Ana R; Donohue, Kevin V; Khalil, Sayed M S; Scholl, Elizabeth; Opperman, Charles; Sonenshine, Daniel E; Roe, R Michael

    2011-01-01

    Many species of mites and ticks are of agricultural and medical importance. Much can be learned from the study of transcriptomes of acarines which can generate DNA-sequence information of potential target genes for the control of acarine pests. High throughput transcriptome sequencing can also yield sequences of genes critical during physiological processes poorly understood in acarines, i.e., the regulation of female reproduction in mites. The predatory mite, Phytoseiulus persimilis, was selected to conduct a transcriptome analysis using 454 pyrosequencing. The objective of this project was to obtain DNA-sequence information of expressed genes from P. persimilis with special interest in sequences corresponding to vitellogenin (Vg) and the vitellogenin receptor (VgR). These genes are critical to the understanding of vitellogenesis, and they will facilitate the study of the regulation of mite female reproduction. A total of 12,556 contiguous sequences (contigs) were assembled with an average size of 935bp. From these sequences, the putative translated peptides of 11 contigs were similar in amino acid sequences to other arthropod Vgs, while 6 were similar to VgRs. We selected some of these sequences to conduct stage-specific expression studies to further determine their function. 2010 Elsevier Ltd. All rights reserved.

  15. Standardization of a rearing procedure of Tetranychus urticae Koch (Acari: Tetranychidae) on bean (Phaseolus vulgaris): plant age and harvest time

    International Nuclear Information System (INIS)

    Bustos, Alexander; Cantor, Fernando; Cure, Jose R; Rodriguez, Daniel

    2009-01-01

    A rearing technique was standardized to produce Tetranychus urticae Koch on Phaseolus vulgaris (ICA Cerinza variety) as a prey of the predatory mite Phytoseiulus persimilis Athias-Henriot. Two assays were conducted to assess the following variables: the most suitable plant age for mite infestation, and the best time to harvest the mites and re infest the plants. In the first experiment, four, five, six, and seven-week-old plants of P. vulgaris were infested with six T. urticae per foliole. The lower plant stratum exhibited the largest number of mites regardless of plant age. However, four-week old plants had the larger average number of individuals. In the second experiment four-week-old plants were infested with 0.5 female mite/cm 2 of leaf. The number of individuals per instar of T. urticae was recorded weekly. The highest mite production occurred between four and five weeks after infestation, indicating this to be the most suitable for mite harvesting and for plant reinfestation. (author)

  16. Ultrastructure and Pathology of Microsporidium phytoseiuli n. sp. Infecting the Predatory Mite, Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae)

    Science.gov (United States)

    Bjørnson; Steiner; Keddie

    1996-11-01

    Ultrastructure and pathology of Microsporidium phytoseiuli n. sp. infecting the predatory mite Phytoseiulus persimilis Athias-Henriot is described using light and transmission electron microscopy. Infected mites showed no gross, external symptoms. All observed stages of the parasite had unpaired nuclei. Schizonts were commonly observed within nuclei of digestive cells of the ventriculus and within the cytoplasm of cells lining the cecal wall and in muscle tissue underlying it. Sporoblasts and spores occurred in the nuclei and cytoplasm of digestive cells within the ventriculus, in cortical regions of the sub- and supraesophageal ganglia, within the cecal wall and muscle tissue, and in parenchyma cells underlying the cuticle. Mature spores were also observed in developing eggs within gravid females. These were broad- to elongate-ovoid, measured 4.33 ± 0.35 x 1.27 ± 0.15 &mgr;m (electron micrographs), 5.37 ± 0.46 x 2.22 ± 0.17 &mgr;m (fixed and stained), and 5.88 ± 0.34 x 2.22 ± 0.19 &mgr;m (fresh) and had an isolfilar polar filament coiled 12 to 15 times within the posterior two-thirds. Within cells, individual spores appeared to be in direct contact with host cytoplasm, while groups of spores were infrequently observed within interfacial envelopes. Groups of 4, 8, to more than 16 spores were observed by light microscopy, while 8 was the maximum observed by electron microscopy. No spores were observed in Tetranychus urticae, a mite used as food during this study.

  17. Process of egg formation in the female body cavity and fertilization in male eggs of Phytoseiulus persimilis (Acari: Phytoseiidae).

    Science.gov (United States)

    Toyoshima, S; Nakamura, M; Nagahama, Y; Amano, H

    2000-01-01

    The process of egg formation in the body cavity of a phytoseiid mite, Phytoseiulus persimilis, was observed to examine fertilization of male eggs. After insemination, one of the ova at the periphery of the ovary began to expand, taking up yolk. Two pronuclei appeared in the expanded egg, located dorsally in the ovary, and yolk granules were formed gradually. After the egg became filled with yolk granules the two pronuclei fused. The egg moved via the narrow entrance at the ventral region into the oviduct, where the eggshell was formed. When the eggshell was complete, and while embryogenesis proceeded, the egg was deposited. In the meantime some ova began to expand sequentially and two joining pronuclei appeared in expanding eggs. The joining pronuclei in the first egg proved male diploidy. This is additional evidence of pseudo-arrhenotoky in this phytoseiid mite species, since the first eggs developed into males.

  18. A Modular Cage System Design for Continuous Medium to Large Scale In Vivo Rearing of Predatory Mites (Acari: Phytoseiidae

    Directory of Open Access Journals (Sweden)

    Juan Alfredo Morales-Ramos

    2014-01-01

    Full Text Available A new stackable modular system was developed for continuous in vivo production of phytoseiid mites. The system consists of cage units that are filled with lima beans,  Phaseolus lunatus, or red beans, P. vulgaris, leaves infested with high levels of the two-spotted spider mites, Tetranychus urticae. The cage units connect with each other through a connection cup, which also serves for monitoring and collection. Predatory mites migrate upwards to new cage units as prey is depleted. The system was evaluated for production of Phytoseiulus persimilis. During a 6-month experimental period, 20,894.9±10,482.5 (mean ± standard deviation predators were produced per week. The production consisted of 4.1±4.6% nymphs and 95.9±4.6% adults. A mean of 554.5±59.8 predatory mites were collected per harvested cage and the mean interval length between harvests was 6.57±6.76 days. The potential for commercial and experimental applications is discussed.

  19. Eriophyid-myte (Acari: Eriophyoidea: Eriophyidae as moontlike beheeragente van ongewenste uitheemse plante in Suid-Afrika

    Directory of Open Access Journals (Sweden)

    C. Craemer

    1996-07-01

    Full Text Available Eriophyid-myte kom waarskynlik op die meeste hoër plante voor en is oor die algemeen baie gasheerspesifiek. Sommiges is skadelik genoeg om oorweeg te word vir biologiese beheerprogramme teen ongewenste plante. Algemene belangstelling in die gebruik van eriophyids vir onkruidbeheer het onlangs ontstaan en vyf spesies is reeds, meestal suksesvol, gebruik vir onkruidbeheer in ander lande. Eriophyid-myte is nog nie as onkruidbeheeragente in Suid-Afrika benut nie. Sewentien spesies wat simptome veroorsaak wat moontlik nuttig kan wees vir die beheer van 12 plantspesies, wat as onkruide in Suid-Afrika beskou word, word gelys en bespreek. Die onkruide is: Acacia saligna (Port Jackson, Acroptilon repens (Russiese dissel, Cardaria draba (peperbos cardaria, Chromolaena odorata (paraffienbos. Convolvulus arvensis (akkerwinde, Cuscuta epithymum (dodder, Hypericum perforatum (St. Janskruid, Lantana camara (lantana, Melia azedarach (sering, Opuntia inermis (doringturksvy, Solanum elaeagnifolium (satansbos, S. mauritianum (luisboom en Spartium junceum (Spaanse besem. Sommige van hierdie eriophyid-spesies kan moontlik suksesvol as onkruidbeheeragente in Suid-Afrika gebruik word en derhalwe is verdere navorsing in hierdie verband geregverdig.

  20. The genus Galumna in Nepalese oribatid mite fauna, with notes on systematic placement of some species (Acari, Oribatida, Galumnidae

    Directory of Open Access Journals (Sweden)

    Sergey Ermilov

    2014-09-01

    Full Text Available The oribatid mite genus Galumna (Oribatida, Galumnidae is recorded for the first time in Nepal. A new species, Galumna tetraporosa sp. n., is described from soil of secondary mixed broadleaved forest. It is most similar morphologically to G. tokyoensis Aoki, 1966 and G. valida Aoki, 1994, however, it differs from both by the absence of interlamellar setae and the presence of two pairs of notogastral porose areas Aa. Galumna granalata Aoki, 1984 is redescribed on the basis of specimens from Nepal. Galumna floridae (Jacot, 1929 and G. hexagona Balogh, 1960 are transferred to the genus Notogalumna; G. mauritii Mahunka, 1978 is transferred to the genus Dimidiogalumna.