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Sample records for density gradient technique

  1. Comparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa

    Institute of Scientific and Technical Information of China (English)

    Shyam S.R. Allamaneni; Ashok Agarwal; Sreedhar Rama; Pavithra Ranganathan; Rakesh K. Sharma

    2005-01-01

    Aim: To 1) compare post-wash and post-thaw parameters of sperm processed with PureSperm density gradient technique and swim-up method; and 2) test the efficacy of two commonly available density gradient media PureSperm and Isolate. Methods: This prospective study used semen specimens from 22 patients. Specimens from nine patients were processed by both PureSperm density gradient and swim-up method. These specimens were then cryopreserved.Thirteen specimens were processed by both PureSperm (40 % and 80 %) and Isolate (50 % and 90 %) double density gradient techniques. The two fractions processed by both PureSperm and swim-up were analyzed for post-wash sperm characteristics. Post-thaw analysis was done after 24 hours. Sperm fractions obtained after processing with PureSperm and Isolate were compared for post-wash sperm characteristics and ROS levels. Results: Specimens prepared with PureSperm had significantly higher median total motile sperm counts (TMSC) (32.2 × 106 vs.17.6 × 106), recovery rates (69.2 % vs. 50.0 %), and longevity at 4 hours (83.0 % vs. 55.0 %) compared to specimen prepared by swim-up. Post-thaw specimens also had a higher recovery and longevity at 4 hours with PureSperm as compared to the swim-up. Semen specimens processed by PureSperm had significantly higher total sperm count,TMSC, and percentage recovery rates (30.0 % vs. 19.7 %) than Isolate. Conclusion: Semen quality is better preserved in fresh and cryopreserved semen prepared with PureSperm density gradient compared to swim-up. A significant enrichment of sperm is observed with PureSperm compared to Isolate. Higher recovery rates of mature motile sperm obtained after PureSperm sperm preparation may be beneficial for successful ART.

  2. A novel technique for real-time estimation of edge pedestal density gradients via reflectometer time delay data

    Science.gov (United States)

    Zeng, L.; Doyle, E. J.; Rhodes, T. L.; Wang, G.; Sung, C.; Peebles, W. A.; Bobrek, M.

    2016-11-01

    A new model-based technique for fast estimation of the pedestal electron density gradient has been developed. The technique uses ordinary mode polarization profile reflectometer time delay data and does not require direct profile inversion. Because of its simple data processing, the technique can be readily implemented via a Field-Programmable Gate Array, so as to provide a real-time density gradient estimate, suitable for use in plasma control systems such as envisioned for ITER, and possibly for DIII-D and Experimental Advanced Superconducting Tokamak. The method is based on a simple edge plasma model with a linear pedestal density gradient and low scrape-off-layer density. By measuring reflectometer time delays for three adjacent frequencies, the pedestal density gradient can be estimated analytically via the new approach. Using existing DIII-D profile reflectometer data, the estimated density gradients obtained from the new technique are found to be in good agreement with the actual density gradients for a number of dynamic DIII-D plasma conditions.

  3. Energy in density gradient

    CERN Document Server

    Vranjes, J

    2015-01-01

    Inhomogeneous plasmas and fluids contain energy stored in inhomogeneity and they naturally tend to relax into lower energy states by developing instabilities or by diffusion. But the actual amount of energy in such inhomogeneities has remained unknown. In the present work the amount of energy stored in a density gradient is calculated for several specific density profiles in a cylindric configuration. This is of practical importance for drift wave instability in various plasmas, and in particular in its application in models dealing with the heating of solar corona because the instability is accompanied with stochastic heating, so the energy contained in inhomogeneity is effectively transformed into heat. It is shown that even for a rather moderate increase of the density at the axis in magnetic structures in the corona by a factor 1.5 or 3, the amount of excess energy per unit volume stored in such a density gradient becomes several orders of magnitude greater than the amount of total energy losses per unit ...

  4. Energy in density gradient

    Energy Technology Data Exchange (ETDEWEB)

    Vranjes, J., E-mail: jvranjes@yahoo.com [Instituto de Astrofísica de Canarias, 38205 La Laguna, Tenerife (Spain); Departamento de Astrofísica, Universidad de La Laguna, 38205 La Laguna, Tenerife (Spain); Kono, M., E-mail: kono@fps.chuo-u.ac.jp [Faculty of Policy Studies, Chuo University, Tokyo (Japan)

    2015-01-15

    Inhomogeneous plasmas and fluids contain energy stored in inhomogeneity and they naturally tend to relax into lower energy states by developing instabilities or by diffusion. But the actual amount of energy in such inhomogeneities has remained unknown. In the present work, the amount of energy stored in a density gradient is calculated for several specific density profiles in a cylindrical configuration. This is of practical importance for drift wave instability in various plasmas, and, in particular, in its application in models dealing with the heating of solar corona because the instability is accompanied with stochastic heating, so the energy contained in inhomogeneity is effectively transformed into heat. It is shown that even for a rather moderate increase of the density at the axis in magnetic structures in the corona by a factor 1.5 or 3, the amount of excess energy per unit volume stored in such a density gradient becomes several orders of magnitude greater than the amount of total energy losses per unit volume (per second) in quiet regions in the corona. Consequently, within the life-time of a magnetic structure such energy losses can easily be compensated by the stochastic drift wave heating.

  5. Density gradient multilayered polymerization (DGMP): a novel technique for creating multi-compartment, customizable scaffolds for tissue engineering.

    Science.gov (United States)

    Joshi-Barr, Shivanjali; Karpiak, Jerome V; Ner, Yogesh; Wen, Jessica H; Engler, Adam J; Almutairi, Adah

    2013-02-12

    Complex tissue culture matrices, in which types and concentrations of biological stimuli (e.g. growth factors, inhibitors, or small molecules) or matrix structure (e.g. composition, concentration, or stiffness of the matrix) vary over space, would enable a wide range of investigations concerning how these variables affect cell differentiation, migration, and other phenomena. The major challenge in creating layered matrices is maintaining the structural integrity of layer interfaces without diffusion of individual components from each layer. Current methodologies to achieve this include photopatterning, lithography, sequential functionalization5, freeze drying, microfluidics, or centrifugation, many of which require sophisticated instrumentation and technical skills. Others rely on sequential attachment of individual layers, which may lead to delamination of layers. DGMP overcomes these issues by using an inert density modifier such as iodixanol to create layers of varying densities. Since the density modifier can be mixed with any prepolymer or bioactive molecule, DGMP allows each scaffold layer to be customized. Simply varying the concentration of the density modifier prevents mixing of adjacent layers while they remain aqueous. Subsequent single step polymerization gives rise to a structurally continuous multilayered scaffold, in which each layer has distinct chemical and mechanical properties. The density modifier can be easily removed with sufficient rinsing without perturbation of the individual layers or their components. This technique is therefore well suited for creating hydrogels of various sizes, shapes, and materials. A protocol for fabricating a 2D-polyethylene glycol (PEG) gel, in which alternating layers incorporate RGDS-350, is outlined below. We use PEG because it is biocompatible and inert. RGDS, a cell adhesion peptide, is used to demonstrate spatial restriction of a biological cue, and the conjugation of a fluorophore (Alexa Fluor 350) enables

  6. GPS, GNSS, and Ionospheric Density Gradients

    Science.gov (United States)

    Kintner, P. M.; O'Hanlon, B.; Humphreys, T. E.

    2009-12-01

    Ionospheric density and density gradients affect GNSS signals in two ways. They can introduce ranging errors or irregularities that form on the density gradients producing scintillation. Here we focus on the issue of ranging errors. There are two approaches to mitigating ranging errors produced by ionospheric density gradients which can be 20-30 m during major magnetic storms. The first approach is to use a reference receiver(s) to determine the ionospheric contribution to ranging errors. The ranging error is then transmitted to the user for correction within the mobile receiver. This approach is frequently referred to as differential GPS and, when multiple reference receivers are used, the system is referred to as an augmentation system. This approach is vulnerable to ionospheric gradients depending on the reference receiver spacing(s) and latency in applying the correction within the mobile receiver. The second approach is to transmit navigation signals at two frequencies and then use the relative delay between the two signals to both estimate the ranging error and calculate the correct range. Currently the dual frequency technique is used by US military receivers with an encryption key and some civilian receivers which must be stationary and average over times long compared to those required for navigation. However, the technology of space based radio navigation is changing. GPS will soon be a system with three frequencies and multiple codes. Furthermore Europe, Russia, and China are developing independent systems to complement and compete with GPS while India and Japan are developing local systems to enhance GPS performance in their regions. In this talk we address two questions. How do density gradients affect augmentation systems including the social consequences and will the new GPS/GNSS systems with multiple civilian frequencies be able to remove ionospheric errors. The answers are not at all clear.

  7. Clinical efficacy of a combination of Percoll continuous density gradient and swim-up techniques for semen processing in HIV-1 serodiscordant couples

    Directory of Open Access Journals (Sweden)

    Osamu Inoue

    2017-01-01

    Full Text Available To evaluate the clinical efficacy of a procedure comprising a combination of Percoll continuous density gradient and modified swim-up techniques for the removal of human immunodeficiency virus type 1 (HIV-1 from the semen of HIV-1 infected males, a total of 129 couples with an HIV-1 positive male partner and an HIV-1 negative female partner (serodiscordant couples who were treated at Keio University Hospital between January 2002 and April 2012 were examined. A total of 183 ejaculates from 129 HIV-1 infected males were processed. After swim-up, we successfully collected motile sperms at a recovery rate as high as 100.0% in cases of normozoospermia (126/126 ejaculates, oligozoospermia (6/6, and asthenozoospermia (36/36. The recovery rate of oligoasthenozoospermia was 86.7% (13/15. In processed semen only four ejaculates (4/181:2.2% showed viral nucleotide sequences consistent with those in the blood of the infected males. After using these sperms, no horizontal infections of the female patients and no vertical infections of the newborns were observed. Furthermore, no obvious adverse effects were observed in the offspring. This protocol allowed us to collect HIV-1 negative motile sperms at a high rate, even in male factor cases. We concluded that our protocol is clinically effective both for decreasing HIV-1 infections and for yielding a healthy child.

  8. Clinical efficacy of a combination of Percoll continuous density gradient and swim-up techniques for semen processing in HIV-1 serodiscordant couples

    Science.gov (United States)

    Inoue, Osamu; Kuji, Naoaki; Ito, Hiroe; Yamada, Mitsutoshi; Hamatani, Toshio; Oyadomari, Aimi; Kato, Shingo; Hanabusa, Hideji; Isaka, Keiichi; Tanaka, Mamoru

    2017-01-01

    To evaluate the clinical efficacy of a procedure comprising a combination of Percoll continuous density gradient and modified swim-up techniques for the removal of human immunodeficiency virus type 1 (HIV-1) from the semen of HIV-1 infected males, a total of 129 couples with an HIV-1 positive male partner and an HIV-1 negative female partner (serodiscordant couples) who were treated at Keio University Hospital between January 2002 and April 2012 were examined. A total of 183 ejaculates from 129 HIV-1 infected males were processed. After swim-up, we successfully collected motile sperms at a recovery rate as high as 100.0% in cases of normozoospermia (126/126 ejaculates), oligozoospermia (6/6), and asthenozoospermia (36/36). The recovery rate of oligoasthenozoospermia was 86.7% (13/15). In processed semen only four ejaculates (4/181:2.2%) showed viral nucleotide sequences consistent with those in the blood of the infected males. After using these sperms, no horizontal infections of the female patients and no vertical infections of the newborns were observed. Furthermore, no obvious adverse effects were observed in the offspring. This protocol allowed us to collect HIV-1 negative motile sperms at a high rate, even in male factor cases. We concluded that our protocol is clinically effective both for decreasing HIV-1 infections and for yielding a healthy child. PMID:26908065

  9. Effects of Density Gradients on Braneworld Stars

    Science.gov (United States)

    Ovalle, Jorge

    During the last few years the braneworld consequences on general relativity have been studied with great interest. The implications in both, cosmological and astrophysics scenarios, have been considered for many authors. However there are some aspects of braneworld consequences which have not been clearly elucidated yet. For instance, the role played for density gradients in the astrophysics scenario is not clear so far, leaving thus the study of braneworld stars as one of the most difficult scenarios. Here it is shown an approach which allows the study of density gradients and their consequences through the Weyl fluid produced inside a stellar distributions. Some general aspects are discussed in detail.

  10. Interplay between transport barriers and density gradient

    Science.gov (United States)

    Sarazin, Y.; Grandgirard, V.; Dif-Pradalier, G.; Garbet, X.; Ghendrih, Ph.

    2006-09-01

    The present paper addresses two critical issues of zonal flows: the evidence of control parameters of their driving term, namely the Reynolds stress, and how they back-react on turbulence and transport. Kinetic nonlinear simulations are performed with the GYSELA code [V. Grandgirard et al., J. Comput. Phys. (to be published)], which models the slab branch of the ion temperature gradient driven instability in the four-dimensional drift-kinetic regime. First, the numerical results show that the gradient of the guiding center density, related to the general potential vorticity, is stabilizing both linearly, by increasing the instability threshold, and nonlinearly, by activating zonal flows. Accordingly, the Reynolds stress is found to scale like LΩ-2 in the quasilinear regime, LΩ being the gradient length of the guiding center density. Second, the local temperature gradient appears to increase linearly with the curvature of the zonal flows, regardless of its sign. Such behavior agrees qualitatively with a perturbative theory. Indeed, while linear eigenmodes are localized at the maximum of the temperature gradient in the absence of zonal flows, they tend to be expelled if both exhibit a maximum at the same location. In this case, the reduction mechanism of the turbulent transport results from the ability of large zonal flow curvatures to render strong temperature gradients stable with respect to perturbations.

  11. Effect of Crustal Density Structures on GOCE Gravity Gradient Observables

    Directory of Open Access Journals (Sweden)

    Robert Tenzer and Pavel Novák

    2013-01-01

    Full Text Available We investigate the gravity gradient components corrected for major known anomalous density structures within the _ crust. Heterogeneous mantle density structures are disregarded. The gravimetric forward modeling technique is utilized to compute the gravity gradients based on methods for a spherical harmonic analysis and synthesis of a gravity field. The _ gravity gradient components are generated using the global geopotential model GOCO-03s. The topographic and stripping gravity corrections due to the density contrasts of the ocean and ice are computed from the global topographic/bathymetric model DTM2006.0 (which also includes the ice-thickness dataset. The discrete data of sediments and crust layers taken from the CRUST2.0 global crustal model are then used to apply the additional stripping corrections for sediments and remaining anomalous crustal density structures. All computations are realized globally on a one arc-deg geographical grid at a mean satellite elevation of 255 km. The global map of the consolidated crust-stripped gravity gradients reveals distinctive features which are attributed to global tectonics, lithospheric plate configuration, lithosphere structure and mantle dynamics (e.g., glacial isostatic adjustment, mantle convection. The Moho signature, which is the most pronounced signal in these refined gravity gradients, is superimposed over a weaker gravity signal of the lithospheric mantle. An interpretational quality of the computed (refined gravity gradient components is mainly limited by a low accuracy and resolution of the CRUST2.0 sediment and crustal layer data and unmodeled mantle structures.

  12. Regularized Multitask Learning for Multidimensional Log-Density Gradient Estimation.

    Science.gov (United States)

    Yamane, Ikko; Sasaki, Hiroaki; Sugiyama, Masashi

    2016-07-01

    Log-density gradient estimation is a fundamental statistical problem and possesses various practical applications such as clustering and measuring nongaussianity. A naive two-step approach of first estimating the density and then taking its log gradient is unreliable because an accurate density estimate does not necessarily lead to an accurate log-density gradient estimate. To cope with this problem, a method to directly estimate the log-density gradient without density estimation has been explored and demonstrated to work much better than the two-step method. The objective of this letter is to improve the performance of this direct method in multidimensional cases. Our idea is to regard the problem of log-density gradient estimation in each dimension as a task and apply regularized multitask learning to the direct log-density gradient estimator. We experimentally demonstrate the usefulness of the proposed multitask method in log-density gradient estimation and mode-seeking clustering.

  13. Effect of Crustal Density Structures on GOCE Gravity Gradient Observables

    Directory of Open Access Journals (Sweden)

    Robert Tenzer Pavel Novák

    2013-01-01

    Full Text Available We investigate the gravity gradient components corrected for major known anomalous density structures within the Earth¡¦s crust. Heterogeneous mantle density structures are disregarded. The gravimetric forward modeling technique is utilized to compute the gravity gradients based on methods for a spherical harmonic analysis and synthesis of a gravity field. The Earth¡¦s gravity gradient components are generated using the global geopotential model GOCO-03s. The topographic and stripping gravity corrections due to the density contrasts of the ocean and ice are computed from the global topographic/bathymetric model DTM2006.0 (which also includes the ice-thickness dataset. The discrete data of sediments and crust layers taken from the CRUST2.0 global crustal model are then used to apply the additional stripping corrections for sediments and remaining anomalous crustal density structures. All computations are realized globally on a one arc-deg geographical grid at a mean satellite elevation of 255 km. The global map of the consolidated crust-stripped gravity gradients reveals distinctive features which are attributed to global tectonics, lithospheric plate configuration, lithosphere structure and mantle dynamics (e.g., glacial isostatic adjustment, mantle convection. The Moho signature, which is the most pronounced signal in these refined gravity gradients, is superimposed over a weaker gravity signal of the lithospheric mantle. An interpretational quality of the computed (refined gravity gradient components is mainly limited by a low accuracy and resolution of the CRUST2.0 sediment and crustal layer data and unmodeled mantle structures.

  14. Planar gradient coil design by scaling the spatial frequencies of minimum-inductance current density.

    Science.gov (United States)

    Lee, S Y; Park, B S; Yi, J H; Yi, W

    1997-11-01

    Gradient coil inductance has been remarkably reduced by the minimum-inductance design technique, which minimizes the magnetic energy stored by the gradient coil. The planar gradient coil designed by this technique, however, often has poor magnetic field linearity. Scaling the spatial frequencies of the current density function derived by this method, the magnetic field linearity of the planar gradient coil can be greatly improved with a small sacrifice of gradient coil inductance. A figure of merit of the planar gradient coil has been found to be improved by scaling the spatial frequencies.

  15. Density Gradient Stabilization of Electron Temperature Gradient Driven Turbulence in a Spherical Tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Ren, Y; Mazzucato, E; Guttenfelder, W; Bell, R E; Domier, C W; LeBlanc, B P; Lee, K C; Luhmann Jr, N C; Smith, D R

    2011-03-21

    In this letter we report the first clear experimental observation of density gradient stabilization of electron temperature gradient driven turbulence in a fusion plasma. It is observed that longer wavelength modes, k⊥ρs ≤10, are most stabilized by density gradient, and the stabilization is accompanied by about a factor of two decrease in the plasma effective thermal diffusivity.

  16. Variation of DNA Fragmentation Levels During Density Gradient Sperm Selection for Assisted Reproduction Techniques: A Possible New Male Predictive Parameter of Pregnancy?

    Science.gov (United States)

    Muratori, Monica; Tarozzi, Nicoletta; Cambi, Marta; Boni, Luca; Iorio, Anna Lisa; Passaro, Claudia; Luppino, Benedetta; Nadalini, Marco; Marchiani, Sara; Tamburrino, Lara; Forti, Gianni; Maggi, Mario; Baldi, Elisabetta; Borini, Andrea

    2016-05-01

    Predicting the outcome of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) is one main goal of the present research on assisted reproduction. To understand whether density gradient centrifugation (DGC), used to select sperm, can affect sperm DNA integrity and impact pregnancy rate (PR), we prospectively evaluated sperm DNA fragmentation (sDF) by TUNEL/PI, before and after DGC. sDF was studied in a cohort of 90 infertile couples the same day of IVF/ICSI treatment. After DGC, sDF increased in 41 samples (Group A, median sDF value: 29.25% [interquartile range, IQR: 16.01-41.63] in pre- and 60.40% [IQR: 32.92-93.53] in post-DGC) and decreased in 49 (Group B, median sDF value: 18.84% [IQR: 13.70-35.47] in pre- and 8.98% [IQR: 6.24-15.58] in post-DGC). PR was 17.1% and 34.4% in Group A and B, respectively (odds ratio [OR]: 2.58, 95% confidence interval [CI]: 0.95-7.04, P = 0.056). After adjustment for female factor, female and male age and female BMI, the estimated OR increased to 3.12 (95% CI: 1.05-9.27, P = 0.041). According to the subgroup analysis for presence/absence of female factor, heterogeneity in the association between the Group A and B and PR emerged (OR: 4.22, 95% CI: 1.16-15.30 and OR: 1.53, 95% CI: 0.23-10.40, respectively, for couples without, n = 59, and with, n = 31, female factor).This study provides the first evidence that the DGC procedure produces an increase in sDF in about half of the subjects undergoing IVF/ICSI, who then show a much lower probability of pregnancy, raising concerns about the safety of this selection procedure. Evaluation of sDF before and after DGC configures as a possible new prognostic parameter of pregnancy outcome in IVF/ICSI. Alternative sperm selection strategies are recommended for those subjects who undergo the damage after DGC.

  17. Particle transport in density gradient driven TE mode turbulence

    CERN Document Server

    Skyman, Andreas; Strand, P I

    2011-01-01

    The turbulent transport of main ion and trace impurities in a tokamak device in the presence of steep electron density gradients has been studied. The parameters are chosen for trapped electron (TE) mode turbulence, driven primarily by steep electron density gradients relevant to H-mode physics, but with a transition to temperature gradient driven turbulence as the density gradient flattens. Results obtained through non-linear (NL) and quasilinear (QL) gyrokinetic simulations using the GENE code are compared with results obtained from a fluid model. Main ion and impurity transport is studied by examining the balance of convective and diffusive transport, as quantified by the density gradient corresponding to zero particle flux (peaking factor). Scalings are obtained for the impurity peaking with the background electron density gradient and the impurity charge number. It is shown that the impurity peaking factor is weakly dependent on impurity charge and significantly smaller than the driving electron density ...

  18. A density gradient theory based method for surface tension calculations

    DEFF Research Database (Denmark)

    Liang, Xiaodong; Michelsen, Michael Locht; Kontogeorgis, Georgios

    2016-01-01

    The density gradient theory has been becoming a widely used framework for calculating surface tension, within which the same equation of state is used for the interface and bulk phases, because it is a theoretically sound, consistent and computationally affordable approach. Based on the observation...... that the optimal density path from the geometric mean density gradient theory passes the saddle point of the tangent plane distance to the bulk phases, we propose to estimate surface tension with an approximate density path profile that goes through this saddle point. The linear density gradient theory, which...... assumes linearly distributed densities between the two bulk phases, has also been investigated. Numerical problems do not occur with these density path profiles. These two approximation methods together with the full density gradient theory have been used to calculate the surface tension of various...

  19. Separation of colloidal two dimensional materials by density gradient ultracentrifugation

    Energy Technology Data Exchange (ETDEWEB)

    Kuang, Yun; Song, Sha [State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029 (China); Huang, Jinyang, E-mail: huangjy@mail.buct.edu.cn [Department of Mathematics, College of Science, Beijing University of Chemical Technology, Beijing 100029 (China); Sun, Xiaoming, E-mail: sunxm@mail.buct.edu.cn [State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029 (China)

    2015-04-15

    Two-dimensional (2D) materials have been made through various approaches but obtaining monodispersed simply by synthesis optimization gained little success, which highlighted the need for introducing nanoseparation methods. Density gradient ultracentrifugation method has emerged as a versatile and scalable method for sorting colloidal 2D nanomaterials. Isopycnic separation was applied on thickness-dependent separation of graphene nanosheets. And rate-zonal separation, as a more versatile separation method, demonstrated its capability in sorting nanosheets of chemically modified single layered graphene, layered double hydroxide, and even metallic Ag. Establishing such density gradient ultracentrifugation method not only achieves monodispersed nanosheets and provides new opportunities for investigation on size dependent properties of 2D materials, but also makes the surface modification possible by introducing “reaction zones” during sedimentation of the colloids. - Graphical abstract: Two-dimensional (2D) materials have been made through various approaches but obtaining monodispersed simply by synthesis optimization gained little success, which highlighted the need for introducing nanoseparation methods. Density gradient ultracentrifugation method has emerged as a versatile and scalable method for sorting colloidal 2D nanomaterials according to their size of thickness difference. Establishing such density gradient ultracentrifugation method not only achieves monodispersed nanosheets and provides new opportunities for investigation on size dependent properties of 2D materials, but also makes the surface modification possible by introducing “reaction zones” during sedimentation of the colloids. - Highlights: • Density gradient ultracentrifugation was applied on size separation of 2D material. • Isopycnic separation was applied on separation of low density materials. • Rate-zonal separation was applied on separation of large density materials. • Size

  20. Analytical gradients for excitation energies from frozen-density embedding.

    Science.gov (United States)

    Kovyrshin, Arseny; Neugebauer, Johannes

    2016-08-21

    The formulation of analytical excitation-energy gradients from time-dependent density functional theory within the frozen-density embedding framework is presented. In addition to a comprehensive mathematical derivation, we discuss details of the numerical implementation in the Slater-function based Amsterdam Density Functional (ADF) program. Particular emphasis is put on the consistency in the use of approximations for the evaluation of second- and third-order non-additive kinetic-energy and exchange-correlation functional derivatives appearing in the final expression for the excitation-energy gradient. We test the implementation for different chemical systems in which molecular excited-state potential-energy curves are affected by another subsystem. It is demonstrated that the analytical implementation for the evaluation of excitation-energy gradients yields results in close agreement with data from numerical differentiation. In addition, we show that our analytical results are numerically more stable and thus preferable over the numerical ones.

  1. Density-gradient-assisted centrifugal microfluidics: an approach to continuous-mode particle separation.

    Science.gov (United States)

    Ukita, Yoshiaki; Oguro, Takayuki; Takamura, Yuzuru

    2017-06-01

    Centrifugal microfluidics has been recognized as a promising pumping method in microfluidics because of its simplicity, easiness of automation, and parallel processing. However, the patterning of stripe flow in centrifugal microfluidics is challenging because a fluid is significantly affected by the Coriolis force, which produces an intrinsic secondary flow. This paper reports a technical and design strategy for centrifugal microfluidics called "density-gradient-assisted centrifugal microfluidics." The flow behavior is observed with the presence of a density gradient and without a density gradient in two concentrically traveling phase flows. As a result, clear stripe flow pattern is observed with a density difference of 0.05 g/cm(3) between water and a percoll solution at a flow rate of 11.8 μl/s (7 ml/10 min) and spinning speed of 3000 rpm. In contrast, without a density gradient, it is necessary to reduce the flow rate and spinning speed to 0.1 μl/s and 1000 rpm, respectively. This paper also presents the use of a density gradient to assist in focusing resin (polystyrene) particles on the boundary of a stripe flow pattern that consists of water and percoll with different densities. Moreover, the density-based separation and sorting of particles in a mixed particle suspension is demonstrated. Polystyrene is selectively focused on the boundary, but silica particles are separated from the focused trajectory due to a difference in density. The separated particles are continuously sorted into different reservoirs with polystyrene and silica separation efficiencies of 96.5% and 98.5%, respectively. The pumping, stripe flow pattern formation, particle concentration, and sorting are simultaneously realized by applying a density gradient and centrifugal force. Therefore, this principle can realize a very simple technique for label-free particle separation by just spinning a disk device and can be applied in other applications by the use of the density-gradient

  2. Fabrication and evaluation of uniform and gradient density epoxies

    Energy Technology Data Exchange (ETDEWEB)

    Domeier, L.A.; Skala, D.M.; Goods, S.H. [and others

    1997-11-01

    Filled epoxy materials which vary in density in a designed manner have been fabricated and their mechanical properties evaluated. Density variations were produced by incorporating different volume fractions of either glass microballoons (GMB) or alumina. Several different sample types were evaluated including uniform density (0.8 g/cm{sup 3} < {rho} < 2.0 g/cm{sup 3}) samples and gradient density samples (GMB only, 0.8 g/cm{sup 3} < {rho} < 1.2 g/cm{sup 3}). The uniform density specimens were evaluated for the effects of filler type and concentration on modulus and toughness. Results indicated that addition of alumina filler significantly increased the resulting modulus while addition of GMB had little measurable effect. These differences could be understood in terms of the differing moduli of the additives relative to that of the epoxy matrix. In the former case the alumina particulates had a modulus much greater than that of the epoxy while in the latter case, the modulus of the GMB additive was only slightly greater than that of the matrix. Addition of either filler significantly degraded the toughness of the composite specimens and precluded the use of gradients to enhance toughness performance. Discontinuous {open_quotes}block{close_quotes} gradients used for testing were fabricated by simple sequential pours of formulations with different GMB loadings and were evaluated for modulus, strength and ductility. Continuous gradients were fabricated in process studies by programmed shifts in the peristaltic pumping/mixing ratio of epoxies filled with either alumina or GMB. None of the continuous gradient materials were mechanically tested. These results suggest that applications utilizing gradient materials containing alumina and similar high modulus fillers to provide designed stiffness rather than improved toughness are the most appropriate targets for future investigation.

  3. Enhancement of electric and magnetic wave fields at density gradients

    Directory of Open Access Journals (Sweden)

    A. Reiniusson

    2006-03-01

    Full Text Available We use Freja satellite data to investigate irregular small-scale density variations. The observations are made in the auroral region at about 1000-1700 km. The density variations are a few percent, and the structures are found to be spatial down to a scale length of a few ion gyroradii. Irregular density variations are often found in an environment of whistler mode/lower hybrid waves and we show that at the density gradients both the electric and magnetic wave fields are enhanced.

  4. The causal relation between turbulent particle flux and density gradient

    Energy Technology Data Exchange (ETDEWEB)

    Milligen, B. Ph. van; Martín de Aguilera, A.; Hidalgo, C. [CIEMAT - Laboratorio Nacional de Fusión, Avda. Complutense 40, 28040 Madrid (Spain); Carreras, B. A. [BACV Solutions, 110 Mohawk Road, Oak Ridge, Tennessee 37830 (United States); García, L.; Nicolau, J. H. [Universidad Carlos III, 28911 Leganés, Madrid (Spain)

    2016-07-15

    A technique for detecting the causal relationship between fluctuating signals is used to investigate the relation between flux and gradient in fusion plasmas. Both a resistive pressure gradient driven turbulence model and experimental Langmuir probe data from the TJ-II stellarator are studied. It is found that the maximum influence occurs at a finite time lag (non-instantaneous response) and that quasi-periodicities exist. Furthermore, the model results show very long range radial influences, extending over most of the investigated regions, possibly related to coupling effects associated with plasma self-organization. These results clearly show that transport in fusion plasmas is not local and instantaneous, as is sometimes assumed.

  5. Interacting Eigenmodes of a plasma diode with a density gradient

    Energy Technology Data Exchange (ETDEWEB)

    Loefgren, T.; Gunell, H.

    1997-08-01

    The formation of narrow high frequency electric field spikes in plasma density gradients is investigated using one-dimensional particle in cell simulations. It is found that the shape of the plasma density gradient is very important for the spike formation. The spike appears also in simulations with immobile ions showing that a coupling to the ion motion, as for example in wave interactions, is not necessary for the formation of HF spikes. However, the HF spike influences the ion motion, and ion waves are seen in the simulations. It has been found, in experiments and simulations, that the electron velocity distribution function deviates from the Maxwellian distribution. Dispersion relations are calculated using realistic distribution functions. The spike can be seen as a coupled system of two Eigenmodes of a plasma diode fed by the beam-plasma interaction. Based on a simplified fluid description of such Eigenmodes, explanations for the localization of the spike, spatially and in frequency, are given. The density amplitude is comparable with the DC density level close to the cathode. Space charge limits of waves in this region seem to determine the amplitude of the spike through the Poisson`s equation. 12 refs, 19 figs.

  6. Nondestructive testing by ESPI and quasi phase shift gradient technique

    Science.gov (United States)

    Pang, Linyong; Wu, Xiaoping

    1996-09-01

    A new nondestructive testing (NDT) technique, which is based on Electronic Speckle Pattern Interferometry (ESPI) and digital image processing with quasi phase shift and gradient technique, is presented. A simple phase reduction algorithm is developed, which replaced an accurate phase shifter. Compared with other phase shift techniques, this method is insensitive to environmental vibration and air disturbance, has visible procedures and results allows the object to move slowly during the inspection procedure, does not need phase unwrapping, and has a quick image processing speed. As an application, this NDT technique is used to detect defects in composite materials and the resulting deformation phase gradient image shows a better visual effect than normal ESPI.

  7. The density gradient effect on quantum Weibel instability

    Energy Technology Data Exchange (ETDEWEB)

    Mahdavi, M., E-mail: m.mahdavi@umz.ac.ir; Khodadadi Azadboni, F., E-mail: f.khodadadi@stu.umz.ac.ir [Physics Department, University of Mazandaran, P. O. Box 47415-416, Babolsar (Iran, Islamic Republic of)

    2015-03-15

    The Weibel instability plays an important role in stopping the hot electrons and energy deposition mechanism in the fast ignition of inertial fusion process. In this paper, the effects of the density gradient and degeneracy on Weibel instability growth rate are investigated. Calculations show that decreasing the density degenerate in the plasma corona, near the relativistic electron beam emitting region by 8.5% leads to a 92% reduction in the degeneracy parameter and about 90% reduction in Weibel instability growth rate. Also, decreasing the degenerate density near the fuel core by 8.5% leads to 1% reduction in the degeneracy parameter and about 8.5% reduction in Weibel instability growth rate. The Weibel instability growth rate shrinks to zero and the deposition condition of relativistic electron beam energy can be shifted to the fuel core for a suitable ignition by increasing the degeneracy parameter in the first layer of plasma corona.

  8. Mineral density volume gradients in normal and diseased human tissues.

    Directory of Open Access Journals (Sweden)

    Sabra I Djomehri

    Full Text Available Clinical computed tomography provides a single mineral density (MD value for heterogeneous calcified tissues containing early and late stage pathologic formations. The novel aspect of this study is that, it extends current quantitative methods of mapping mineral density gradients to three dimensions, discretizes early and late mineralized stages, identifies elemental distribution in discretized volumes, and correlates measured MD with respective calcium (Ca to phosphorus (P and Ca to zinc (Zn elemental ratios. To accomplish this, MD variations identified using polychromatic radiation from a high resolution micro-computed tomography (micro-CT benchtop unit were correlated with elemental mapping obtained from a microprobe X-ray fluorescence (XRF using synchrotron monochromatic radiation. Digital segmentation of tomograms from normal and diseased tissues (N=5 per group; 40-60 year old males contained significant mineral density variations (enamel: 2820-3095 mg/cc, bone: 570-1415 mg/cc, cementum: 1240-1340 mg/cc, dentin: 1480-1590 mg/cc, cementum affected by periodontitis: 1100-1220 mg/cc, hypomineralized carious dentin: 345-1450 mg/cc, hypermineralized carious dentin: 1815-2740 mg/cc, and dental calculus: 1290-1770 mg/cc. A plausible linear correlation between segmented MD volumes and elemental ratios within these volumes was established, and Ca/P ratios for dentin (1.49, hypomineralized dentin (0.32-0.46, cementum (1.51, and bone (1.68 were observed. Furthermore, varying Ca/Zn ratios were distinguished in adapted compared to normal tissues, such as in bone (855-2765 and in cementum (595-990, highlighting Zn as an influential element in prompting observed adaptive properties. Hence, results provide insights on mineral density gradients with elemental concentrations and elemental footprints that in turn could aid in elucidating mechanistic processes for pathologic formations.

  9. Density Gradient Ultracentrifugation to Isolate Endogenous Protein Complexes after Affinity Capture.

    Science.gov (United States)

    Fernandez-Martinez, Javier; LaCava, John; Rout, Michael P

    2016-07-01

    This protocol describes the isolation of native protein complexes by density gradient ultracentrifugation. The outcome of an affinity capture and native elution experiment is generally a mixture of (1) the complex(es) associated with the protein of interest under the specific conditions of capture, (2) fragments of the complex generated by degradation or disassembly during the purification procedure, and (3) the protease or reagent used to natively elute the sample. To separate these components and isolate a homogeneous complex, an additional step of purification is required. Rate-zonal density gradient ultracentrifugation is a reliable and powerful technique for separating particles based on their hydrodynamic volume. The density gradient is generated by mixing low- and high-density solutions of a suitable low-molecular-weight inert solute (e.g., sucrose or glycerol). The gradient is formed in a solvent that could be any of the solvents used for the affinity capture and native elution and should help to preserve the structure and activity of the assembly.

  10. Moho Density Contrast in Central Eurasia from GOCE Gravity Gradients

    Directory of Open Access Journals (Sweden)

    Mehdi Eshagh

    2016-05-01

    Full Text Available Seismic data are primarily used in studies of the Earth’s inner structure. Since large parts of the world are not yet sufficiently covered by seismic surveys, products from the Earth’s satellite observation systems have more often been used for this purpose in recent years. In this study we use the gravity-gradient data derived from the Gravity field and steady-state Ocean Circulation Explorer (GOCE, the elevation data from the Shuttle Radar Topography Mission (SRTM and other global datasets to determine the Moho density contrast at the study area which comprises most of the Eurasian plate (including parts of surrounding continental and oceanic tectonic plates. A regional Moho recovery is realized by solving the Vening Meinesz-Moritz’s (VMM inverse problem of isostasy and a seismic crustal model is applied to constrain the gravimetric solution. Our results reveal that the Moho density contrast reaches minima along the mid-oceanic rift zones and maxima under the continental crust. This spatial pattern closely agrees with that seen in the CRUST1.0 seismic crustal model as well as in the KTH1.0 gravimetric-seismic Moho model. However, these results differ considerably from some previously published gravimetric studies. In particular, we demonstrate that there is no significant spatial correlation between the Moho density contrast and Moho deepening under major orogens of Himalaya and Tibet. In fact, the Moho density contrast under most of the continental crustal structure is typically much more uniform.

  11. Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells

    Directory of Open Access Journals (Sweden)

    Yuxi Sun

    2017-08-01

    Full Text Available Density-gradient centrifugation is a label-free approach that has been extensively used for cell separations. Though elegant, this process is time-consuming (>30 min, subjects cells to high levels of stress (>350 g and relies on user skill to enable fractionation of cells that layer as a narrow band between the density-gradient medium and platelet-rich plasma. We hypothesized that microfluidic adaptation of this technique could transform this process into a rapid fractionation approach where samples are separated in a continuous fashion while being exposed to lower levels of stress (<100 g for shorter durations of time (<3 min. To demonstrate proof-of-concept, we designed a microfluidic density-gradient centrifugation device and constructed a setup to introduce samples and medium like Ficoll in a continuous, pump-less fashion where cells and particles can be exposed to centrifugal force and separated via different outlets. Proof-of-concept studies using binary mixtures of low-density polystyrene beads (1.02 g/cm3 and high-density silicon dioxide beads (2.2 g/cm3 with Ficoll–Paque (1.06 g/cm3 show that separation is indeed feasible with >99% separation efficiency suggesting that this approach can be further adapted for separation of cells.

  12. Stable smoothed particle magnetohydrodynamics in very steep density gradients

    CERN Document Server

    Lewis, Benjamin T; Monaghan, Joseph J; Price, Daniel J

    2015-01-01

    The equations of smoothed particle magnetohydrodynamics (SPMHD), even with the various corrections to instabilities so far proposed, have been observed to be unstable when a very steep density gradient is necessarily combined with a variable smoothing length formalism. Here we consider in more detail the modifications made to the SPMHD equations in LBP2015 that resolve this instability by replacing the smoothing length in the induction and anisotropic force equations with an average smoothing length term. We then explore the choice of average used and compare the effects on a test `cylinder-in-a-box' problem and the collapse of a magnetised molecular cloud core. We find that, aside from some benign numerical effects at low resolutions for the quadratic mean, the formalism is robust as to the choice of average but that in complicated models it is essential to apply the average to both equations; in particular, all four averages considered exhibit similar conservation properties. This improved formalism allows ...

  13. Weighted graph based ordering techniques for preconditioned conjugate gradient methods

    Science.gov (United States)

    Clift, Simon S.; Tang, Wei-Pai

    1994-01-01

    We describe the basis of a matrix ordering heuristic for improving the incomplete factorization used in preconditioned conjugate gradient techniques applied to anisotropic PDE's. Several new matrix ordering techniques, derived from well-known algorithms in combinatorial graph theory, which attempt to implement this heuristic, are described. These ordering techniques are tested against a number of matrices arising from linear anisotropic PDE's, and compared with other matrix ordering techniques. A variation of RCM is shown to generally improve the quality of incomplete factorization preconditioners.

  14. Bull Fertility and Its Relation with Density Gradient Selected Sperm

    Directory of Open Access Journals (Sweden)

    Allouche Lynda ,

    2017-01-01

    Full Text Available Background Sperm selection method is usually used to collect these cells for in vitro-assisted reproduction. Few studies reported the relationship of in vivo fertility and semen parameters after sperm selection; hence, the present study attempted to assess different semen parameters after post-thaw or sperm selection, using density gradient separation BoviPure®, to predict in vivo fertility. Materials and Methods In this experimental study, frozen semen quality of four Montbeliarde bulls were assessed after post-thaw (PT or after sperm selection (SSp, using density gradient separation BoviPure®, to predict the fertility rate in vivo. In addition to PT or SSp, semen was examined for concentration, motility, morphology abnormalities, viability, acrosome and plasma membrane integrities. Fertility was measured as non-return rates within 56 days after the first insemination (NRR or as corrected NRR, expressed as CNRR, to the factors influencing fertility using linear mixed model. Non-parametric Kruskal-Wallis test was performed to compare semen parameter variables. Fertility rates were compared using Chi-square test. Pearson correlation analysis was used to test the relationship between CNRR and semen parameters. Data was analysed using SPSS package program, version 21.0. Results Most of the examined bulls exhibited a high fertility rate (3/4 bulls, 62.1- 81.8% for NRR or 67.2-98.5% for CNRR. Fertility rate, expressed as CNRR, was significantly related to semen parameters after SSp, but not after PT. Thus, CNRR was increased with decrease of total motility, progressive spermatozoa and abaxial implantation frequencies after SSp (r=-0.999, P=0.001; r=-0.990, P=0.010; r=-0.988, P= 0.012, respectively; while, CNRR was decreased with decrease of SSp immotile spermatozoa (r=+0.995, P=0.005, underlying that maximal limit of determined immotile spermatozoa is 47%. Conclusion High frequencies of total and progressive motility spermatozoa, and abaxial

  15. [gamma]-ray CT examination of density gradients in slip ceramics: correlation with stress distribution

    Energy Technology Data Exchange (ETDEWEB)

    Sawicka, B.D. (AECL Research, Chalk River Labs., Ontario (Canada)); Murphy, J.G.; Taheri, F.; Kanary, L.E. (Advanced Materials Engineering Centre, Halifax, Nova Scotia (Canada))

    1992-06-01

    Residual stresses caused by processing techniques reduce the safe allowable design life of components. This is especially critical for brittle materials, like ceramics, which do not exhibit plastic deformation. During development of the manufacturing process for an experimental high-precision ceramic component, a characteristic cracking pattern occurred in a series of prototypes, which suggested development of stresses during densification, either during drying or sintering. To examine parameters which may influence the development of these residual stresses, a series of simple geometry specimens were prepared and characterized for density gradients using computed tomography (CT). Using the measured values of density gradients, a model of the sintering process was made and the resultant stress distribution in the parts calculated. Results indicate that the use of nonlinear finite element analysis in conjunction with hypoelastic materials modeling qualitatively represents the sintering stresses.

  16. γ-ray CT examination of density gradients in slip cast ceramics: correlation with stress distribution

    Science.gov (United States)

    Sawicka, B. D.; Murphy, J. G.; Taheri, F.; Kanary, L. E.

    1992-06-01

    Residual stresses caused by processing techniques reduce the safe allowable design life of components. This is especially critical for brittle materials, like ceramics, which do not exhibit plastic deformation. During development of the manufacturing process for an experimental high-precision ceramic component, a characteristic cracking pattern occurred in a series of prototypes, which suggested development of stresses during densification, either during drying or sintering. To examine parameters which may influence the development of these residual stresses, a series of simple geometry specimens were prepared and characterized for density gradients using computed tomography (CT). Using the measured values of density gradients, a model of the sintering process was made and the resultant stress distribution in the parts calculated. Results indicate that the use of nonlinear finite element analysis in conjunction with hypoelastic materials modelling qualitatively represents the sintering stresses.

  17. Layout-Driven Post-Placement Techniques for Temperature Reduction and Thermal Gradient Minimization

    DEFF Research Database (Denmark)

    Liu, Wei; Calimera, Andrea; Macii, Alberto;

    2013-01-01

    With the continuing scaling of CMOS technology, on-chip temperature and thermal-induced variations have become a major design concern. To effectively limit the high temperature in a chip equipped with a cost-effective cooling system, thermal specific approaches, besides low power techniques......, are necessary at the chip design level. The high temperature in hotspots and large thermal gradients are caused by the high local power density and the nonuniform power dissipation across the chip. With the objective of reducing power density in hotspots, we propose two placement techniques that spread cells...... layout. We compare the proposed methods in terms of temperature reduction, timing, and area overhead to the baseline method, which enlarges the circuit area uniformly. The experimental results showed that our methods achieve a larger reduction in both peak temperature and thermal gradient than...

  18. Gram-scale fractionation of nanodiamonds by density gradient ultracentrifugation

    Science.gov (United States)

    Peng, Wei; Mahfouz, Remi; Pan, Jun; Hou, Yuanfang; Beaujuge, Pierre M.; Bakr, Osman M.

    2013-05-01

    Size is a defining characteristic of nanoparticles; it influences their optical and electronic properties as well as their interactions with molecules and macromolecules. Producing nanoparticles with narrow size distributions remains one of the main challenges to their utilization. At this time, the number of practical approaches to optimize the size distribution of nanoparticles in many interesting materials systems, including diamond nanocrystals, remains limited. Diamond nanocrystals synthesized by detonation protocols - so-called detonation nanodiamonds (DNDs) - are promising systems for drug delivery, photonics, and composites. DNDs are composed of primary particles with diameters mainly Applications requiring DNDs with specific particle or aggregate sizes are now within reach.Size is a defining characteristic of nanoparticles; it influences their optical and electronic properties as well as their interactions with molecules and macromolecules. Producing nanoparticles with narrow size distributions remains one of the main challenges to their utilization. At this time, the number of practical approaches to optimize the size distribution of nanoparticles in many interesting materials systems, including diamond nanocrystals, remains limited. Diamond nanocrystals synthesized by detonation protocols - so-called detonation nanodiamonds (DNDs) - are promising systems for drug delivery, photonics, and composites. DNDs are composed of primary particles with diameters mainly Applications requiring DNDs with specific particle or aggregate sizes are now within reach. Electronic supplementary information (ESI) available: Discussion of the influence of sample solution concentrations on DLS measurements, comparisons of the size distributions of our raw milled particles and NanoAmando particles, a detailed description of the RZDGU procedure, discussion of the influences of the gradients and centrifugation times on fractionation, TEM images, zeta potentials, AUC analysis and

  19. Gram-scale fractionation of nanodiamonds by density gradient ultracentrifugation

    KAUST Repository

    Peng, Wei

    2013-01-01

    Size is a defining characteristic of nanoparticles; it influences their optical and electronic properties as well as their interactions with molecules and macromolecules. Producing nanoparticles with narrow size distributions remains one of the main challenges to their utilization. At this time, the number of practical approaches to optimize the size distribution of nanoparticles in many interesting materials systems, including diamond nanocrystals, remains limited. Diamond nanocrystals synthesized by detonation protocols-so-called detonation nanodiamonds (DNDs)-are promising systems for drug delivery, photonics, and composites. DNDs are composed of primary particles with diameters mainly <10 nm and their aggregates (ca. 10-500 nm). Here, we introduce a large-scale approach to rate-zonal density gradient ultracentrifugation to obtain monodispersed fractions of nanoparticles in high yields. We use this method to fractionate a highly concentrated and stable aqueous solution of DNDs and to investigate the size distribution of various fractions by dynamic light scattering, analytical ultracentrifugation, transmission electron microscopy and powder X-ray diffraction. This fractionation method enabled us to separate gram-scale amounts of DNDs into several size ranges within a relatively short period of time. In addition, the high product yields obtained for each fraction allowed us to apply the fractionation method iteratively to a particular size range of particles and to collect various fractions of highly monodispersed primary particles. Our method paves the way for in-depth studies of the physical and optical properties, growth, and aggregation mechanism of DNDs. Applications requiring DNDs with specific particle or aggregate sizes are now within reach. © 2013 The Royal Society of Chemistry.

  20. Inhomogeneous radiation degradation in polymers studied with a density gradient column

    Science.gov (United States)

    Gillen, K. T.; Clough, R. L.

    Radiation dose-rate effects are known to exist in many polymers and are typically caused by oxidation reactions. Since the buildup of oxidation products often leads to an increase in polymer density, a density gradient column was used in a novel way to study the mechanisms responsible for dose-rate effects in a commercial ethylene propylene rubber (EPR) electrical cable insulation material. After various aging conditions, density profiles of samples were obtained by measuring the density of small pieces cut successively from the outside to the inside of the approximately 1-mm thick insulation. By monitoring density profiles as a function of dose rate and total radiation dose, two inhomogeneous mechanisms were found to be responsible for the observed dose-rate effects in this EPR material. At high dose rates, oxygen-diffusion-limited degradation was identified, as evidenced by a density profile which showed increased density near the air-exposed surfaces of the insulation but little change in density in the interior of the sample. As the dose rate was lowered, the oxidation region spread inward until it encompassed the entire sample at dose rates below about 100 krad/hr. The second mechanism, responsible for continuing the dose-rate effects at lower dose rates, appears to involve copper-catalyzed oxidation. This mechanism is often found to be significant in thermal aging studies; evidence that it may also be significant under room-temperature, radiation-aging conditions came from the density profile results, which revealed large increases in density as the inside of the insulation (near the tinned-copper conductor) was approached. For the same total radiation dose, this increase became larger as the dose rate was lowered. Preliminary chemical analysis profiling techniques indicate significantly enhanced copper and tin concentrations as the inside of the insulation is approached, even for insulation stripped from the metallic conductor before aging. This implies that

  1. Calculate Electric Field Gradient of TiO2 Within Density Functional Theory

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    <正>TiO2 electric field gradient has been calculated utilizing WIEN2K program, which is ab initio based on density function theory (DFT). DFT uses the charge density as a variable instead of electronic wave

  2. A numerical study on charged-particle scattering and radiography of a steep density gradient

    Science.gov (United States)

    Shao, Guangchao; Wang, Xiaofang

    2016-09-01

    Electron and proton radiography of polystyrene planar targets with different density gradients is studied by Monte Carlo simulations in a regime that the incident charged-particle's kinetic energy is much higher than its energy loss in the targets. It is shown that by scattering of the electrons or protons, the density gradient causes modulations of the charged-particle beam transmitted from the target and the modulation contrast is sensitive only to a steep gradient, which suggests a novel diagnostic method wherein a steep density gradient could be distinguished from the scattering of a charged-particle beam in radiography. By using a 100-MeV charged-particle beam, it is found that the modulation is evident for a steep density gradient of width smaller than 1 μm for electron radiography and 0.6 μm for proton radiography, respectively, but almost negligible when the density gradient width is greater than 1 μm. The feasibility of diagnosing the steep density gradients in compressed matter is confirmed by the simulations of radiographing a laser-ablated planar foil. Simulations also show that it is possible to diagnose the density gradients inside a multilayered spherical capsule.

  3. Stabilization of electron-scale turbulence by electron density gradient in national spherical torus experiment

    Energy Technology Data Exchange (ETDEWEB)

    Ruiz Ruiz, J.; White, A. E. [MIT-Plasma Science and Fusion Center, Cambridge, Massachusetts 02139 (United States); Ren, Y.; Guttenfelder, W.; Kaye, S. M.; Leblanc, B. P.; Mazzucato, E. [Princeton Plasma Physics Laboratory, Princeton, New Jersey 08543 (United States); Lee, K. C. [National Fusion Research Institute, Daejeon (Korea, Republic of); Domier, C. W. [University of California at Davis, Davis, California 95616 (United States); Smith, D. R. [University of Wisconsin-Madison, Madison, Wisconsin 53706 (United States); Yuh, H. [Nova Photonics, Inc., Princeton, New Jersey 08540 (United States)

    2015-12-15

    Theory and experiments have shown that electron temperature gradient (ETG) turbulence on the electron gyro-scale, k{sub ⊥}ρ{sub e} ≲ 1, can be responsible for anomalous electron thermal transport in NSTX. Electron scale (high-k) turbulence is diagnosed in NSTX with a high-k microwave scattering system [D. R. Smith et al., Rev. Sci. Instrum. 79, 123501 (2008)]. Here we report on stabilization effects of the electron density gradient on electron-scale density fluctuations in a set of neutral beam injection heated H-mode plasmas. We found that the absence of high-k density fluctuations from measurements is correlated with large equilibrium density gradient, which is shown to be consistent with linear stabilization of ETG modes due to the density gradient using the analytical ETG linear threshold in F. Jenko et al. [Phys. Plasmas 8, 4096 (2001)] and linear gyrokinetic simulations with GS2 [M. Kotschenreuther et al., Comput. Phys. Commun. 88, 128 (1995)]. We also found that the observed power of electron-scale turbulence (when it exists) is anti-correlated with the equilibrium density gradient, suggesting density gradient as a nonlinear stabilizing mechanism. Higher density gradients give rise to lower values of the plasma frame frequency, calculated based on the Doppler shift of the measured density fluctuations. Linear gyrokinetic simulations show that higher values of the electron density gradient reduce the value of the real frequency, in agreement with experimental observation. Nonlinear electron-scale gyrokinetic simulations show that high electron density gradient reduces electron heat flux and stiffness, and increases the ETG nonlinear threshold, consistent with experimental observations.

  4. A density-adaptive SPH method with kernel gradient correction for modeling explosive welding

    Science.gov (United States)

    Liu, M. B.; Zhang, Z. L.; Feng, D. L.

    2017-05-01

    Explosive welding involves processes like the detonation of explosive, impact of metal structures and strong fluid-structure interaction, while the whole process of explosive welding has not been well modeled before. In this paper, a novel smoothed particle hydrodynamics (SPH) model is developed to simulate explosive welding. In the SPH model, a kernel gradient correction algorithm is used to achieve better computational accuracy. A density adapting technique which can effectively treat large density ratio is also proposed. The developed SPH model is firstly validated by simulating a benchmark problem of one-dimensional TNT detonation and an impact welding problem. The SPH model is then successfully applied to simulate the whole process of explosive welding. It is demonstrated that the presented SPH method can capture typical physics in explosive welding including explosion wave, welding surface morphology, jet flow and acceleration of the flyer plate. The welding angle obtained from the SPH simulation agrees well with that from a kinematic analysis.

  5. Density gradient centrifugation in urografin of Moraxella and Kingella cells and appendages.

    Science.gov (United States)

    Frøholm, L O; Bøvre, K

    1978-04-01

    Purification of fimbriae (pili) by density gradient banding in Urografin medium was attempted. Moraxella nonliquefaciens and Kingella kingae fimbriae were of higher density than their cells of origin, but fimbrial fractions obtained by homogenization and differential centrifugation still banded together with presumed outer membrane fragments and some whole cells in Urografin gradients. The cellular density of genetic variants with different fimbriation/competence levels was also studied. For one strain of M. nonliquefaciens and two strains of K. kingae, cells harvested from agar plates tended to show several bands on isopycnic density gradient centrifugation, with slightly higher general density of fimbriated variants than non-fimbriated. A single density band could be observed with cells from log phase broth cultures of selected strains which showed no distinct difference between fimbriation or competence variants of each strain. Cells of M. nonliquefaciens and M. bovis showed comparable buoyant densities, whereas those of K. kingae had a higher density.

  6. Cell Growth on ("Janus") Density Gradients of Bifunctional Zeolite L Crystals.

    Science.gov (United States)

    Kehr, Nermin Seda; Motealleh, Andisheh; Schäfer, Andreas H

    2016-12-28

    Nanoparticle density gradients on surfaces have attracted interest as two-dimensional material surfaces that can mimic the complex nano-/microstructure of the native extracellular matrix, including its chemical and physical gradients, and can therefore be used to systematically study cell-material interactions. In this respect, we report the preparation of density gradients made of bifunctional zeolite L crystals on glass surfaces and the effects of the density gradient and biopolymer functionalization of zeolite L crystals on cell adhesion. We also describe how we created "Janus" density gradient surfaces by gradually depositing two different types of zeolite L crystals that were functionalized and loaded with different chemical groups and guest molecules onto the two distinct sides of the same glass substrate. Our results show that more cells adhered on the density gradient of biopolymer-coated zeolites than on uncoated ones. The number of adhered cells increased up to a certain surface coverage of the glass by the zeolite L crystals, but then it decreased beyond the zeolite density at which a higher surface coverage decreased fibroblast cell adhesion and spreading. Additionally, cell experiments showed that cells gradually internalized the guest-molecule-loaded zeolite L crystals from the underlying density gradient containing bifunctional zeolite L crystals.

  7. BMP4 density gradient in disk-shaped confinement

    Science.gov (United States)

    Bozorgui, Behnaz; Teimouri, Hamid; Kolomeisky, Anatoly B.

    We present a quantitative model that explains the scaling of BMP4 gradients during gastrulation and the recent experimental observation that geometric confinement of human embryonic stem cells is sufficient to recapitulate much of germ layer patterning. Based on a assumption that BMP4 diffusion rate is much smaller than the diffusion rate of it's inhibitor molecules, our results confirm that the length-scale which defines germ layer territories does not depend on system size.

  8. Integration of the Density Gradient Model into a General Purpose Device Simulator

    Directory of Open Access Journals (Sweden)

    Andreas Wettstein

    2002-01-01

    Full Text Available A generalized Density Gradient model has been implemented into the device simulator Dessis [DESSIS 7.0 reference manual (2001. ISE Integrated Systems Engineering AG, Balgriststrasse 102, CH-8008 Zürich].We describe the multidimensional discretization scheme used and discuss our modifications to the standard Density Gradient model. The evaluation of the model shows good agreement to results obtained by the Schro¨dinger equation.

  9. Arrays of quasi-hexagonally ordered silica nanopillars with independently controlled areal density, diameter and height gradients

    Science.gov (United States)

    Özdemir, Burcin; Huang, Wenting; Plettl, Alfred; Ziemann, Paul

    2015-03-01

    A consecutive fabrication approach of independently tailored gradients of the topographical parameters distance, diameter and height in arrays of well-ordered nanopillars on smooth SiO2-Si-wafers is presented. For this purpose, previously reported preparation techniques are further developed and combined. First, self-assembly of Au-salt loaded micelles by dip-coating with computer-controlled pulling-out velocities and subsequent hydrogen plasma treatment produce quasi-hexagonally ordered, 2-dimensional arrays of Au nanoparticles (NPs) with unidirectional variations of the interparticle distances along the pulling direction between 50-120 nm. Second, the distance (or areal density) gradient profile received in this way is superimposed with a diameter-controlled gradient profile of the NPs applying a selective photochemical growth technique. For demonstration, a 1D shutter is used for locally defined UV exposure times to prepare Au NP size gradients varying between 12 and 30 nm. Third, these double-gradient NP arrangements serve as etching masks in a following reactive ion etching step delivering arrays of nanopillars. For height gradient generation, the etching time is locally controlled by applying a shutter made from Si wafer piece. Due to the high flexibility of the etching process, the preparation route works on various materials such as cover slips, silicon, silicon oxide, silicon nitride and silicon carbide.

  10. Density functional theory for molecular and periodic systems using density fitting and continuous fast multipole method: Analytical gradients.

    Science.gov (United States)

    Łazarski, Roman; Burow, Asbjörn Manfred; Grajciar, Lukáš; Sierka, Marek

    2016-10-30

    A full implementation of analytical energy gradients for molecular and periodic systems is reported in the TURBOMOLE program package within the framework of Kohn-Sham density functional theory using Gaussian-type orbitals as basis functions. Its key component is a combination of density fitting (DF) approximation and continuous fast multipole method (CFMM) that allows for an efficient calculation of the Coulomb energy gradient. For exchange-correlation part the hierarchical numerical integration scheme (Burow and Sierka, Journal of Chemical Theory and Computation 2011, 7, 3097) is extended to energy gradients. Computational efficiency and asymptotic O(N) scaling behavior of the implementation is demonstrated for various molecular and periodic model systems, with the largest unit cell of hematite containing 640 atoms and 19,072 basis functions. The overall computational effort of energy gradient is comparable to that of the Kohn-Sham matrix formation. © 2016 Wiley Periodicals, Inc.

  11. Density gradients and internal dust in the Orion nebula

    Directory of Open Access Journals (Sweden)

    Luc Binette

    2002-01-01

    Full Text Available La estructura de ionización de la nebulosa de Orión puede ser descrita como una piel delgada sobre la superficie de una nube densa. Proponemos que una estratificación en la densidad, descrita por una ley de potencias (n / x_2, donde x es la distancia al frente de ionización, presenta propiedades que concuerdan con nuestros espectros de rendija larga de la nebulosa de Orión. Por ejemplo, existe una relación de unicidad entre el brillo superficial en HB, o la densidad del frente de ionización en [S II], y la escala L de la ley de potencias, donde L es la distancia entre el frente de ionización y el lugar donde comienza a crecer la densidad, cerca de la estrella excitadora. Es necesario incluir polvo interno a fin de obtener un ajuste aceptable a las observaciones tanto de la densidad del [S II], como del brillo superficial en HB. Los modelos que incluyen granos de polvo pequeños proporcionan un mejor ajuste que aquellos con granos grandes. Los gradientes de los cocientes de líneas observados a lo largo de la rendija se reproducen cualitativamente por nuestros modelos de densidad estratificada, suponiendo una temperatura estelar de 38,000 K. La desexcitación colisional parece ser la responsable de la mitad del gradiente observado en el cociente [N II] 5755/[N II] 6583, el cual es sensible a la temperatura. Proponemos que la relación empírica encontrada por Wen & O`Dell (1995 entre la densidad y la distancia a la estrella puede deberse a una estratificación de la densidad en forma de ley de potencias.

  12. Predicting Chemical Reactivity from the Charge Density through Gradient Bundle Analysis: Moving beyond Fukui Functions.

    Science.gov (United States)

    Morgenstern, Amanda; Wilson, Timothy R; Eberhart, M E

    2017-06-08

    Predicting chemical reactivity is a major goal of chemistry. Toward this end, atom condensed Fukui functions of conceptual density functional theory have been used to predict which atom is most likely to undergo electrophilic or nucleophilic attack, providing regioselectivity information. We show that the most probable regions for electrophilic attack within each atom can be predicted through analysis of gradient bundle volumes, a property that depends only on the charge density of the neutral molecules. We also introduce gradient bundle condensed Fukui functions to compare the stereoselectivity information obtained from gradient bundle volume analysis. We demonstrate this method using the test set of molecular fluorine, oxygen, nitrogen, carbon monoxide, and hydrogen cyanide.

  13. Horizontal density-gradient effects on simulation of flow and transport in the Potomac Estuary

    Science.gov (United States)

    Schaffranek, Raymond W.; Baltzer, Robert A.; ,

    1990-01-01

    A two-dimensional, depth-integrated, hydrodynamic/transport model of the Potomac Estuary between Indian Head and Morgantown, Md., has been extended to include treatment of baroclinic forcing due to horizontal density gradients. The finite-difference model numerically integrates equations of mass and momentum conservation in conjunction with a transport equation for heat, salt, and constituent fluxes. Lateral and longitudinal density gradients are determined from salinity distributions computed from the convection-diffusion equation and an equation of state that expresses density as a function of temperature and salinity; thus, the hydrodynamic and transport computations are directly coupled. Horizontal density variations are shown to contribute significantly to momentum fluxes determined in the hydrodynamic computation. These fluxes lead to enchanced tidal pumping, and consequently greater dispersion, as is evidenced by numerical simulations. Density gradient effects on tidal propagation and transport behavior are discussed and demonstrated.

  14. Velocity fields as a tracer from magnetic fields in sub-alfv\\'enic regimes: The Velocity Gradient Technique

    CERN Document Server

    González-Casanova, Diego F

    2016-01-01

    Strong Alfv\\'enic turbulence develops eddy-like motions perpendicular to the local direction of magnetic fields. This local alignment induces velocity gradients perpendicular to the local direction of the magnetic field. We use this fact to propose a new technique of studying the direction of magnetic fields from observations, the Velocity Gradient Technique. We test our idea by employing the synthetic observations obtained via 3D MHD numerical simulations for different sonic and Alfv\\'en Mach numbers. We calculate the velocity gradient, $\\mathbf{\\Omega}$, using the velocity centroids. We find that $\\mathbf{\\Omega}$ traces the projected magnetic field best for the synthetic maps obtained with sub-Alfv\\'enic simulations providing good point-wise correspondence between the magnetic field direction and that of $\\mathbf{\\Omega}$. The reported alignment is much better than the alignment between the density gradients and the magnetic field and we demonstrated that it can be used to find the magnetic field strength ...

  15. Effect of density gradient centrifugation on reactive oxygen species in human semen.

    Science.gov (United States)

    Takeshima, Teppei; Yumura, Yasushi; Kuroda, Shinnosuke; Kawahara, Takashi; Uemura, Hiroji; Iwasaki, Akira

    2017-06-01

    Density gradient centrifugation can separate motile sperm from immotile sperm and other cells for assisted reproduction, but may also remove antioxidants from seminal plasma, resulting in oxidative stress. Therefore, we investigated reactive oxygen species (ROS) concentrations and distribution in semen before and after density gradient centrifugation. We assessed semen volume, sperm concentration, sperm motility, and ROS levels before and after density gradient centrifugation (300 x g for 20 minutes) in 143 semen samples from 118 patients. The ROS removal rate was evaluated in ROS-positive samples and ROS formation rate in ROS-negative samples. Thirty-eight of 143 untreated samples (26.6%) were ROS-positive; sperm motility was significantly lower in these samples than in ROS-negative samples (p density gradient centrifugation, only seven of the 38 ROS-positive samples (18.42%) exhibited a ROS-positive lower layer (containing motile sperm) with a ROS removal rate of 81.58%, whereas the upper layer was ROS-positive in 24 samples (63.16%). In the ROS-negative group (n = 105), ROS was detected in 19 samples after centrifugation (18.10%, ROS generation rate), of which 18 were ROS-positive only in the upper layer or interface and the other was ROS-positive in both layers. Density gradient centrifugation can separate motile sperm from immotile sperm as well as remove ROS (including newly generated ROS). This data supports the view that density gradient centrifugation can select motile spermatozoa without enhancing oxidative stress. ROS: reactive oxygen species; SOD: superoxide dismutase; GPx: glutathione peroxidase; DNA: deoxyribonucleic acid; DGC: density gradient centrifugation; IUI: intrauterine insemination; IVF: in vitro fertilization; HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; EDTA: ethylenediaminetetraacetic acid; HTF: HEPES-buffered human tubal fluid; IMSI: intracytoplasmic morphologically selected sperm injection; SMAS: sperm motility analyzing

  16. The influence of mineral matter on the separation of amorphous marine kerogens using density gradient centrifugation

    NARCIS (Netherlands)

    Sinninghe Damsté, J.S.; Hartgers, W.A.; Leeuw, J.W. de; Ling, Y.; Crelling, J.C.

    1995-01-01

    Three relatively immature amorphous marine kerogens were subjected to density gradient centrifugation (DGC). The density fractions obtained were analyzed by Curie-point pyrolysis in combination with gas chromatography (Py-GC) and with gas chromatography-mass spectrometry (Py-GC-MS). Despite the

  17. Space charge profiles in low density polyethylene samples containing a permittivity/conductivity gradient

    DEFF Research Database (Denmark)

    Bambery, K.R.; Fleming, R.J.; Holbøll, Joachim

    2001-01-01

    .5×107 V m-1. Current density was also measured as a function of temperature and field. Space charge due exclusively to the temperature gradient was detected, with density of order 0.01 C m-3. The activation energy associated with the transport of electrons through the bulk was calculated as 0.09 e...

  18. Evaluation of centrifugation parameters for density gradient experiments by means of a programmable pocket calculator.

    Science.gov (United States)

    Kreutzfeldt, C

    1980-10-01

    A calculation program is proposed suitable for programmable pocket calculators (e.g. HP series) to estimate s20,w f omega2 dt values from density gradient centrifugation data. The program can be applied to linear or exponential density gradients prepared from sucrose or glycerol solutions spun in zonal rotors or swinging bucket rotors. A wide solute concentration range and temperature range is accounted for. Constants for empirical density calculation of glycerol and sucrose solutions concentrated in % (w/v) are estimated. Experimental verification of the program was carried out.

  19. Density-Gradient-Driven trapped-electron-modes in improved-confinement RFP plasmas

    Science.gov (United States)

    Duff, James

    2016-10-01

    Short wavelength density fluctuations in improved-confinement MST plasmas exhibit multiple features characteristic of the trapped-electron-mode (TEM), strong evidence that drift wave turbulence emerges in RFP plasmas when transport associated with MHD tearing is reduced. Core transport in the RFP is normally governed by magnetic stochasticity stemming from long wavelength tearing modes that arise from current profile peaking. Using inductive control, the tearing modes are reduced and global confinement is increased to values expected for a comparable tokamak plasma. The improved confinement is associated with a large increase in the pressure gradient that can destabilize drift waves. The measured density fluctuations have frequencies >50 kHz, wavenumbers k_phi*rho_sglobal tearing modes. Their amplitude increases with the local density gradient, and they exhibit a density-gradient threshold at R/L_n 15, higher than in tokamak plasmas by R/a. the GENE code, modified for RFP equilibria, predicts the onset of microinstability for these strong-gradient plasma conditions. The density-gradient-driven TEM is the dominant instability in the region where the measured density fluctuations are largest, and the experimental threshold-gradient is close to the predicted critical gradient for linear stability. While nonlinear analysis shows a large Dimits shift associated with predicted strong zonal flows, the inclusion of residual magnetic fluctuations causes a collapse of the zonal flows and an increase in the predicted transport to a level close to the experimentally measured heat flux. Similar circumstances could occur in the edge region of tokamak plasmas when resonant magnetic perturbations are applied for the control of ELMs. Work supported by US DOE.

  20. Multispecies Density and Temperature Gradient Dependence of Quasilinear Particle and Energy Fluxes

    Energy Technology Data Exchange (ETDEWEB)

    G. Rewoldt; R.V. Budny; W.M. Tang

    2004-08-09

    The variations of the normalized quasilinear particle and energy fluxes with artificial changes in the density and temperature gradients, as well as the variations of the linear growth rates and real frequencies, for ion temperature gradient and trapped-electron modes, are calculated. The quasilinear fluxes are normalized to the total energy flux, summed over all species. Here, realistic cases for tokamaks and spherical torii are considered which have two impurity species. For situations where there are substantial changes in the normalized fluxes, the ''diffusive approximation,'' in which the normalized fluxes are taken to be linear in the gradients, is seen to be inaccurate. Even in the case of small artificial changes in density or temperature gradients, changes in the fluxes of different species (''off-diagonal'') generally are significant, or even dominant, compared to those for the same species (''diagonal'').

  1. Cosmological constraints from the redshift dependence of the Alcock-Paczynski test: galaxy density gradient field

    CERN Document Server

    Li, Xiao-Dong; Forero-Romero, Jaime E; Kim, Juhan

    2014-01-01

    We propose a method based on the redshift dependence of the Alcock-Paczynski (AP) test to measure the expansion history of the Universe. It uses the isotropy of the galaxy density gradient field to constrain cosmological parameters. If the density parameter $\\Omega_m$ or the dark energy equation of state $w$ are incorrectly chosen, the gradient field appears to be anisotropic with the degree of anisotropy varying with redshift. We use this effect to constrain the cosmological parameters governing the expansion history of the Universe. Although redshift-space distortions (RSD) induced by galaxy peculiar velocities also produce anisotropies in the gradient field, these effects are close to uniform in magnitude over a large range of redshift. This makes the redshift variation of the gradient field anisotropy relatively insensitive to the RSD. By testing the method on mock surveys drawn from the Horizon Run 3 cosmological N-body simulations, we demonstrate that the cosmological parameters can be estimated without...

  2. A rapid and scalable density gradient purification method for Plasmodium sporozoites

    Directory of Open Access Journals (Sweden)

    Kennedy Mark

    2012-12-01

    Full Text Available Abstract Background Malaria remains a major human health problem, with no licensed vaccine currently available. Malaria infections initiate when infectious Plasmodium sporozoites are transmitted by Anopheline mosquitoes during their blood meal. Investigations of the malaria sporozoite are, therefore, of clear medical importance. However, sporozoites can only be produced in and isolated from mosquitoes, and their isolation results in large amounts of accompanying mosquito debris and contaminating microbes. Methods Here is described a discontinuous density gradient purification method for Plasmodium sporozoites that maintains parasite infectivity in vitro and in vivo and greatly reduces mosquito and microbial contaminants. Results This method provides clear advantages over previous approaches: it is rapid, requires no serum components, and can be scaled to purify >107 sporozoites with minimal operator involvement. Moreover, it can be effectively applied to both human (Plasmodium falciparum, Plasmodium vivax and rodent (Plasmodium yoelii infective species with excellent recovery rates. Conclusions This novel method effectively purifies viable malaria sporozoites by greatly reducing contaminating mosquito debris and microbial burdens associated with parasite isolation. Large-scale preparations of purified sporozoites will allow for enhanced in vitro infections, proteomics, and biochemical characterizations. In conjunction with aseptic mosquito rearing techniques, this purification technique will also support production of live attenuated sporozoites for vaccination.

  3. Projected gradient algorithms for Hartree-Fock and density matrix functional theory calculations

    Science.gov (United States)

    Cancès, Eric; Pernal, Katarzyna

    2008-04-01

    We present projected gradient algorithms designed for optimizing various functionals defined on the set of N-representable one-electron reduced density matrices. We show that projected gradient algorithms are efficient in minimizing the Hartree-Fock or the Müller-Buijse-Baerends functional. On the other hand, they converge very slowly when applied to the recently proposed BBk (k =1,2,3) functionals [O. Gritsenko et al., J. Chem. Phys. 122, 204102 (2005)]. This is due to the fact that the BBk functionals are not proper functionals of the density matrix.

  4. An Independent Component Analysis Algorithm through Solving Gradient Equation Combined with Kernel Density Estimation

    Institute of Scientific and Technical Information of China (English)

    XUE Yun-feng; WANG Yu-jia; YANG Jie

    2009-01-01

    A new algorithm for linear instantaneous independent component analysis is proposed based on max-imizing the log-likelihood contrast function which can be changed into a gradient equation. An iterative method is introduced to solve this equation efficiently. The unknown probability density functions as well as their first and second derivatives in the gradient equation are estimated by kernel density method. Computer simulations on artificially generated signals and gray scale natural scene images confirm the efficiency and accuracy of the proposed algorithm.

  5. Localization of the Proton Pump of Corn Coleoptile Microsomal Membranes by Density Gradient Centrifugation 1

    Science.gov (United States)

    Mandala, Suzanne; Mettler, Irvin J.; Taiz, Lincoln

    1982-01-01

    Previous studies characterizing an ATP-dependent proton pump in microsomal membrane vesicles of corn coleoptiles led to the conclusion that the proton pump was neither mitochondrial nor plasma membrane in origin (Mettler, Mandala, Taiz 1982 Plant Physiol 70: 1738-1742). To facilitate positive identification of the vesicles, corn coleoptile microsomal membranes were fractionated on linear sucrose and dextran gradients, with ATP-dependent [14C]methylamine uptake as a probe for proton pumping. On sucrose gradients, proton pumping activity exhibited a density of 1.11 grams/cubic centimeter and was coincident with the endoplasmic reticulum (ER). In the presence of high magnesium, the ER shifted to a heavier density, while proton pumping activity showed no density shift. On linear dextran gradients, proton pumping activity peaked at a lighter density than the ER. The proton pump appears to be electrogenic since both [14C]SCN− uptake and 36Cl− uptake activities coincided with [14C] methylamine uptake on dextran gradients. On the basis of density and transport properties, we conclude that the proton pumping vesicles are probably derived from the tonoplast. Nigericin-stimulated ATPase activity showed a broad distribution which did not coincide with any one membrane marker. PMID:16662755

  6. Preparation of Avocado Mitochondria Using Self-Generated Percoll Density Gradients and Changes in Buoyant Density during Ripening.

    Science.gov (United States)

    Moreau, F; Romani, R

    1982-11-01

    Mitochondria from avocado (Persea americana Mill, var. Fuerte and Hass) can be rapidly prepared at every stage of ripening using differential centrifugation and self-generated Percoll gradients. The procedure results in improved oxidative and phosphorylative properties, especially for mitochondria isolated from preclimacteric fruits.A gradual change in the buoyant density of avocado mitochondria takes place during ripening. Climacteric and postclimacteric avocado mitochondria have the same buoyant density as other plant mitochondria (potato, cauliflower), whereas mitochondria from preclimacteric fruit have a lower density. The transition in buoyant density occurs during the climacteric rise, and two populations of intact mitochondria (p = 1.060 and p = 1.075) can be separated at this stage. Evidence indicates that the difference in mitochondrial buoyant density between preclimacteric and postclimacteric mitochondria is likely due to interactions with soluble cytosolic components.

  7. Purification of white spot syndrome virus by iodixanol density gradient centrifugation.

    Science.gov (United States)

    Dantas-Lima, J J; Corteel, M; Cornelissen, M; Bossier, P; Sorgeloos, P; Nauwynck, H J

    2013-10-01

    Up to now, only a few brief procedures for purifying white spot syndrome virus (WSSV) have been described. They were mainly based on sucrose, NaBr and CsCl density gradient centrifugation. This work describes for the first time the purification of WSSV through iodixanol density gradients, using virus isolated from infected tissues and haemolymph of Penaeus vannamei (Boone). The purification from tissues included a concentration step by centrifugation (2.5 h at 60,000 g) onto a 50% iodixanol cushion and a purification step by centrifugation (3 h at 80,000 g) through a discontinuous iodixanol gradient (phosphate-buffered saline, 5%, 10%, 15% and 20%). The purification from infected haemolymph enclosed a dialysis step with a membrane of 1,000 kDa (18 h) and a purification step through the earlier iodixanol gradient. The gradients were collected in fractions and analysed. The number of particles, infectivity titre (in vivo), total protein and viral protein content were evaluated. The purification from infected tissues gave WSSV suspensions with a very high infectivity and an acceptable purity, while virus purified from haemolymph had a high infectivity and a very high purity. Additionally, it was observed that WSSV has an unusually low buoyant density and that it is very sensitive to high external pressures.

  8. Investigation of Vapor-Liquid Nucleation for Associating Fluids by Density Gradient Theory

    Institute of Scientific and Technical Information of China (English)

    FU Dong; LIU Jianmin

    2009-01-01

    An equation of state (EOS) applicable to both the uniform and non-uniform associating fluids was established by using the density-gradient expansion, in which the influence parameter κis formulated as a function of tempera-ture. The molecular parameters were regressed by fitting to the experimental data of vapor pressures and liquid den-sities. Within the framework of density gradient theory (DGT), the nucleation rates for water, heavy water, metha-nol, ethanol, 1-propanoi, 1-butanol, 1-pentanol and 1-hexanol were calculated. The results were satisfactory com-pared with the experimental data. Our study shows that DGT preserves all the advantages of density functional the-ory (DFT) in capturing the structure and properties of nucleus but gives much more accurate nucleation rates by adjusting the influence parameter.

  9. Stabilized density gradient theory algorithm for modeling interfacial properties of pure and mixed systems

    CERN Document Server

    Mu, Xiaoqun; Alpak, Faruk O; Chapman, Walter G

    2016-01-01

    Density gradient theory (DGT) allows fast and accurate determination of surface tension and density profile through a phase interface. Several algorithms have been developed to apply this theory in practical calculations. While the conventional algorithm requires a reference substance of the system, a modified "stabilized density gradient theory" (SDGT) algorithm is introduced in our work to solve DGT equations for multiphase pure and mixed systems. This algorithm makes it possible to calculate interfacial properties accurately at any domain size larger than the interface thickness without choosing a reference substance or assuming the functional form of the density profile. As part of DGT inputs, the perturbed chain statistical associating fluid theory (PC-SAFT) equation of state (EoS) was employed for the first time with the SDGT algorithm. PC-SAFT has excellent performance in predicting liquid phase properties as well as phase behaviors. The SDGT algorithm with the PC-SAFT EoS was tested and compared with ...

  10. Vertical density gradient in the eastern North Atlantic during the last 30,000 years

    Energy Technology Data Exchange (ETDEWEB)

    Rogerson, M.; Ramirez, J. [University of Hull, Geography Department, Hull (United Kingdom); Bigg, G.R. [University of Sheffield, Department of Geography, Sheffield (United Kingdom); Rohling, E.J. [University of Southampton, National Oceanography Centre, School of Ocean and Earth Science, Southampton (United Kingdom)

    2012-08-15

    Past changes in the density and momentum structure of oceanic circulation are an important aspect of changes in the Atlantic Meridional Overturning Circulation and consequently climate. However, very little is known about past changes in the vertical density structure of the ocean, even very extensively studied systems such as the North Atlantic. Here we exploit the physical controls on the settling depth of the dense Mediterranean water plume derived from the Strait of Gibraltar to obtain the first robust, observations-based, probabilistic reconstruction of the vertical density gradient in the eastern North Atlantic during the last 30,000 years. We find that this gradient was weakened by more than 50%, relative to the present, during the last Glacial Maximum, and that changes in general are associated with reductions in AMOC intensity. However, we find only a small change during Heinrich Event 1 relative to the Last Glacial Maximum, despite strong evidence that overturning was substantially altered. This implies that millennial-scale changes may not be reflected in vertical density structure of the ocean, which may be limited to responses on an ocean-overturning timescale or longer. Regardless, our novel reconstruction of Atlantic density structure can be used as the basis for a dynamical measure for validation of model-based AMOC reconstructions. In addition, our general approach is transferrable to other marginal sea outflow plumes, to provide estimates of oceanic vertical density gradients in other locations. (orig.)

  11. Horizontal Ionospheric Electron Density Gradients Observed by FORMOSAT-3/COSMIC TIP: Spatial Distributions and Effects on VLF Wave Propagation at Mid-Latitudes

    Directory of Open Access Journals (Sweden)

    Damien H. Chua

    2009-01-01

    Full Text Available We investigate the spatial variability of electron densities in the nightside ionosphere and its effects on very-low frequency (VLF wave propagation using a suite of instruments from the FORMOSAT-3/Constellation Observing System for Meteorology Ionosphere and Climate (COSMIC spacecraft.We use observations from the Tiny Ionospheric Photometer (TIP instruments to infer the horizontal electron density gradients along each satellite track. We demonstrate that the OI 1356 _ radiance measured by the TIP instruments tracks the horizontal electron density structure well with high spatial resolution and unprecedented sensitivity. Accurate measurements of the horizontal electron density gradients are important for improving retrieved electron density profiles from GPS occultation and other tomographic remote sensing techniques. The processes underlying the variability in the large-scale, nightside electron density gradients are the main drivers of ionospheric weather. TIP observations reveal significant variability in both the small and large scale structure of the nightside ionosphere. The relative intensities, relative widths, and latitudinal separation of the northern and southern ionization crests of the Appleton anomalies show a high degree of longitudinal variation.We demonstrate how the TIP observations can be used to measure the horizontal gradient of the refractive index of whistler-mode VLF waves propagating in a cold, collisionless plasma. These measurements are critical for understanding how gradients in electron density associated with ionospheric structure such as depletions and the Appleton anomalies affect VLF wave propagation through the equatorial and mid-latitude ionosphere.

  12. Dislocation structure of Ge crystals grown by low thermal gradient Czochralski technique

    Science.gov (United States)

    Trukhanov, E. M.; Fritzler, K. B.; Vasilenko, A. P.; Kolesnikov, A. V.; Kasimkin, P. V.; Moskovskih, V. A.

    2017-06-01

    Dislocation structure of the Ge single crystals grown by Czochralski method with low thermal gradient has been studied. The selective etching technique and the X-Ray transmission and reflection topography were used. Clearly defined non-uniform dislocation distribution over the crystal cross - section is revealed. Helical dislocations and sets of prismatic dislocation loops are registered. Helical dislocations perpendicular to the ingot axis are situated near the boundary between the regions with low and high dislocation densities (102 and 103 cm-2, respectively). Their length can be as much as several millimeters. Dislocation formations lying at a 35.3° to the crystal axis along directions are also observed. These formations have the shape of prism confined by {111} planes.

  13. The effect of density gradient on the growth rate of relativistic Weibel instability

    Energy Technology Data Exchange (ETDEWEB)

    Mahdavi, M., E-mail: m.mahdavi@umz.ac.ir [Physics Department, University of Mazandaran, P.O. Box 47415-416, Babolsar (Iran, Islamic Republic of); Khodadadi Azadboni, F., E-mail: f.khodadadi@stu.umz.ac.ir [Physics Department, University of Mazandaran, P.O. Box 47415-416, Babolsar (Iran, Islamic Republic of); Young Researchers Club, Sari Branch, Islamic Azad University, P.O. Box 48161-194, Sari (Iran, Islamic Republic of)

    2014-02-15

    In this paper, the effect of density gradient on the Weibel instability growth rate is investigated. The density perturbations in the near corona fuel, where temperature anisotropy, η, is larger than the critical temperature anisotropy, η{sub c}, (η > η{sub c}), enhances the growth rate of Weibel instability due to the sidebands coupled with the electron oscillatory velocity. But for η < η{sub c}, the thermal spread of the energetic electrons reduces the growth rate. Also, the growth rate can be reduced if the relativistic parameter (Lorentz factor) is sufficiently large, γ > 2. The analysis shows that relativistic effects and density gradient tend to stabilize the Weibel instability. The growth rate can be reduced by 88% by reducing η by a factor of 100 and increasing relativistic parameter by a factor of 3.

  14. Space charge profiles in low density polyethylene samples containing a permittivity/conductivity gradient

    DEFF Research Database (Denmark)

    Bambery, K.R.; Fleming, R.J.; Holbøll, Joachim

    2001-01-01

    Laser induced pressure pulse space charge measurements were made on 1.5 mm thick plaques of high purity low density polyethylene equipped with vacuum-evaporated aluminium electrodes. Temperature differences up to 20 °C were maintained across the samples, which were subjected to dc fields up to 1.......5×107 V m-1. Current density was also measured as a function of temperature and field. Space charge due exclusively to the temperature gradient was detected, with density of order 0.01 C m-3. The activation energy associated with the transport of electrons through the bulk was calculated as 0.09 e...

  15. Relative contributions of temperature and salinity to seasonal mixed layer density changes and horizontal density gradients

    OpenAIRE

    Johnson, Gregory C.; Schmidtko, Sunke; Lyman, John M.

    2012-01-01

    Temperature and salinity both contribute to ocean density, including its seasonal cycle and spatial patterns in the mixed layer. Temperature and salinity profiles from the Argo Program allow construction and analysis of a global, monthly, mixed layer climatology. Temperature changes dominate the seasonal cycle of mixed layer density in most regions, but salinity changes are dominant in the tropical warm pools, Arctic, and Antarctic. Under the Intertropical Convergence Zone, temperature and sa...

  16. A collocation method for surface tension calculations with the density gradient theory

    DEFF Research Database (Denmark)

    Larsen, Peter Mahler; Maribo-Mogensen, Bjørn; Kontogeorgis, Georgios M.

    2016-01-01

    Surface tension calculations are important in many industrial applications and over a wide range of temperatures, pressures and compositions. Empirical parachor methods are not suitable over a wide condition range and the combined use of density gradient theory with equations of state has been...... proposed in literature. Often, many millions of calculations are required in the gradient theory methods, which is computationally very intensive. In this work, we have developed an algorithm to calculate surface tensions an order of magnitude faster than the existing methods, with no loss of accuracy...

  17. Effects of density, velocity gradient, and compressibility on side-jet formation in round jets with variable density

    Science.gov (United States)

    Muramatsu, Akinori

    2013-11-01

    When a low density gas compared with the ambient gas is discharged from a round nozzle, side jets that are radial ejections of jet fluid are generated at the initial region of the jet. The density ratio between the jet fluid and the ambient fluid is a main parameter for the side-jet formation. Since the side-jet formation is also related to the instability of shear layer, it depends on the velocity gradient of the shear layer in the jet. The velocity gradient is evaluated by a ratio of the momentum thickness and the nozzle diameter at the nozzle exit. Compressibility suppresses the instability and the generation of the side jets. The compressibility is evaluated by a Mach number, which is a ratio defined by an issuing velocity of the jet and a sound velocity in the ambient fluid. Influence of these three parameters on the side-jet formation was examined experimentally. The density ratio and momentum thickness ratio were varied from 0.14 to 1.53, and from 14 to 155, respectively. The Mach number was varied to 0.7. Existence of side jets was confirmed by flow visualization using a laser sheet. Domains for the side-jet formation by the density ratio, the momentum thickness ratio, and the Mach number were determined.

  18. Pitfalls of using the geometric-mean combining rule in the density gradient theory

    DEFF Research Database (Denmark)

    Liang, Xiaodong; Michelsen, Michael Locht; Kontogeorgis, Georgios

    2016-01-01

    It is popular and attractive to model the interfacial tension using the density gradient theory with the geometric-mean combining rule, in which the same equation of state is used for the interface and bulk phases. The computational efficiency is the most important advantage of this theory. In th...... the interface could be considered as a warning for the unsuccessful applications of the geometric-mean density gradient theory combined with the chosen thermodynamic model, even if numerical pitfalls do not occur. (C) 2016 Elsevier B.V. All rights reserved.......It is popular and attractive to model the interfacial tension using the density gradient theory with the geometric-mean combining rule, in which the same equation of state is used for the interface and bulk phases. The computational efficiency is the most important advantage of this theory....... In this work, it has been mathematically shown that the theory fails if the solution profile is not monotonic in the path function, which is defined as the summation of the density multiplied by the square root of the influence parameter over all components. A computational solution procedure is then presented...

  19. Baroclinic pressure gradient difference schemes of subtracting the local averaged density stratification in sigma coordinates models

    Institute of Scientific and Technical Information of China (English)

    ZHU Shouxian; ZHANG Wenjing

    2008-01-01

    Much has been written of the error in computing the baroclinic pressure gradient (BPG) with sigma coordinates in ocean or atmos- pheric numerical models. The usual way to reduce the error is to subtract area-averaged density stratification of the whole computa- tion region. But if there is great difference between the area-averaged and the local averaged density stratification, the error will be obvious. An example is given to show that the error from this method may be larger than that from no correction sometimes. The definition of local area is put forward. Then, four improved BPG difference schemes of subtracting the local averaged density strat- ification are designed to reduce the error. Two of them are for diagnostic calculation (density field is fixed), and the others are for prognostic calculation (density field is not fixed). The results show that the errors from these schemes all significantly decrease.

  20. A SELF-ADAPTIVE TECHNIQUE FOR A KIND OF NONLINEAR CONJUGATE GRADIENT METHODS

    Institute of Scientific and Technical Information of China (English)

    王丽平

    2004-01-01

    Conjugate gradient methods. are a class of important methods for unconstrained optimization, especially when the dimension is large. In 2001, Dai and Liao have proposed a new conjugate condition, based on it two nonlinear conjugate gradient methods are constructed. With trust region idea, this paper gives a self-adaptive technique for the two methods. The numerical results show that this technique works well for the given nonlinear optimization test problems.

  1. Effect of semen extender and density gradient centrifugation on the motility and fertility of turkey spermatozoa.

    Science.gov (United States)

    Morrell, J M; Persson, B; Tjellström, H; Laessker, A; Nilsson, H; Danilova, M; Holmes, P V

    2005-12-01

    In the absence of commercially viable methods for cryopreserving turkey spermatozoa, new processing methods are required to extend the functional life of stored turkey spermatozoa for artificial insemination. The present study evaluates the efficacy of a new extender (Turkey Semen Extend) and investigates the use of density gradient centrifugation in processing turkey spermatozoa for artificial insemination. The new extender is compared with two commercially available turkey semen extenders, Beltsville Poultry Semen Extender and Ovodyl. Turkey spermatozoa in Turkey Semen Extend were still motile 20 h after collection, representing a considerable improvement over the other semen extenders (40%, 0% and 8% for Turkey Semen Extend, Beltsville Poultry Semen Extender and Ovodyl, respectively). A field trial on a commercial turkey farm showed improved fertilization rates following insemination of turkey hens with semen extended in Turkey Semen Extend (89.7%) compared with Beltsville Poultry Semen Extender (86.9%). This difference is statistically significant (p < 0.05). Processing on a density gradient, optimized for turkey spermatozoa, also increased sperm survival (50% gradient-prepared spermatozoa still motile after 18 h compared with <10% non-processed spermatozoa). Preliminary studies indicate that gradient preparation of spermatozoa may aid survival during cryopreservation.

  2. Variable density sampling based on physically plausible gradient waveform. Application to 3D MRI angiography

    CERN Document Server

    Chauffert, Nicolas; Boucher, Marianne; Mériaux, Sébastien; CIUCIU, Philippe

    2015-01-01

    Performing k-space variable density sampling is a popular way of reducing scanning time in Magnetic Resonance Imaging (MRI). Unfortunately, given a sampling trajectory, it is not clear how to traverse it using gradient waveforms. In this paper, we actually show that existing methods [1, 2] can yield large traversal time if the trajectory contains high curvature areas. Therefore, we consider here a new method for gradient waveform design which is based on the projection of unrealistic initial trajectory onto the set of hardware constraints. Next, we show on realistic simulations that this algorithm allows implementing variable density trajectories resulting from the piecewise linear solution of the Travelling Salesman Problem in a reasonable time. Finally, we demonstrate the application of this approach to 2D MRI reconstruction and 3D angiography in the mouse brain.

  3. Analytic calculations of hyper-Raman spectra from density functional theory hyperpolarizability gradients

    Energy Technology Data Exchange (ETDEWEB)

    Ringholm, Magnus; Ruud, Kenneth [Centre for Theoretical and Computational Chemistry, Department of Chemistry, University of Tromsø – The Arctic University of Norway, 9037 Tromsø (Norway); Bast, Radovan [Theoretical Chemistry and Biology, School of Biotechnology, Royal Institute of Technology, AlbaNova University Center, S-10691 Stockholm (Sweden); PDC Center for High Performance Computing, Royal Institute of Technology, S-10044 Stockholm (Sweden); Oggioni, Luca [Centre for Theoretical and Computational Chemistry, Department of Chemistry, University of Tromsø – The Arctic University of Norway, 9037 Tromsø (Norway); Department of Physics G. Occhialini, University of Milano Bicocca, Piazza della scienza 3, 20126 Milan (Italy); Ekström, Ulf [Centre for Theoretical and Computational Chemistry, Department of Chemistry, University of Oslo, P.O. Box 1033 Blindern, 0315 Oslo (Norway)

    2014-10-07

    We present the first analytic calculations of the geometrical gradients of the first hyperpolarizability tensors at the density-functional theory (DFT) level. We use the analytically calculated hyperpolarizability gradients to explore the importance of electron correlation effects, as described by DFT, on hyper-Raman spectra. In particular, we calculate the hyper-Raman spectra of the all-trans and 11-cis isomers of retinal at the Hartree-Fock (HF) and density-functional levels of theory, also allowing us to explore the sensitivity of the hyper-Raman spectra on the geometrical characteristics of these structurally related molecules. We show that the HF results, using B3LYP-calculated vibrational frequencies and force fields, reproduce the experimental data for all-trans-retinal well, and that electron correlation effects are of minor importance for the hyper-Raman intensities.

  4. Analytic calculations of hyper-Raman spectra from density functional theory hyperpolarizability gradients

    Science.gov (United States)

    Ringholm, Magnus; Bast, Radovan; Oggioni, Luca; Ekström, Ulf; Ruud, Kenneth

    2014-10-01

    We present the first analytic calculations of the geometrical gradients of the first hyperpolarizability tensors at the density-functional theory (DFT) level. We use the analytically calculated hyperpolarizability gradients to explore the importance of electron correlation effects, as described by DFT, on hyper-Raman spectra. In particular, we calculate the hyper-Raman spectra of the all-trans and 11-cis isomers of retinal at the Hartree-Fock (HF) and density-functional levels of theory, also allowing us to explore the sensitivity of the hyper-Raman spectra on the geometrical characteristics of these structurally related molecules. We show that the HF results, using B3LYP-calculated vibrational frequencies and force fields, reproduce the experimental data for all-trans-retinal well, and that electron correlation effects are of minor importance for the hyper-Raman intensities.

  5. Separation of Bacteria, Protozoa and Carbon Nanotubes by Density Gradient Centrifugation

    Directory of Open Access Journals (Sweden)

    Monika Mortimer

    2016-10-01

    Full Text Available Sustainable production and use of carbon nanotube (CNT-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors. Microbes, as abundant organisms responsible for nutrient cycling in soil and water, are important ecological receptors for studying the effects of CNTs. Quantification of CNT association with microbial cells requires efficient separation of CNT-associated cells from individually dispersed CNTs and CNT agglomerates. Here, we designed, optimized, and demonstrated procedures for separating bacteria (Pseudomonas aeruginosa from unbound multiwall carbon nanotubes (MWCNTs and MWCNT agglomerates using sucrose density gradient centrifugation. We demonstrate separation of protozoa (Tetrahymena thermophila from MWCNTs, bacterial agglomerates, and protozoan fecal pellets by centrifugation in an iodixanol solution. The presence of MWCNTs in the density gradients after centrifugation was determined by quantification of 14C-labeled MWCNTs; the recovery of microbes from the density gradient media was confirmed by optical microscopy. Protozoan intracellular contents of MWCNTs and of bacteria were also unaffected by the designed separation process. The optimized methods contribute to improved efficiency and accuracy in quantifying MWCNT association with bacteria and MWCNT accumulation in protozoan cells, thus supporting improved assessment of CNT bioaccumulation.

  6. Separation of Bacteria, Protozoa and Carbon Nanotubes by Density Gradient Centrifugation

    Science.gov (United States)

    Mortimer, Monika; Petersen, Elijah J.; Buchholz, Bruce A.; Holden, Patricia A.

    2016-01-01

    Sustainable production and use of carbon nanotube (CNT)-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors. Microbes, as abundant organisms responsible for nutrient cycling in soil and water, are important ecological receptors for studying the effects of CNTs. Quantification of CNT association with microbial cells requires efficient separation of CNT-associated cells from individually dispersed CNTs and CNT agglomerates. Here, we designed, optimized, and demonstrated procedures for separating bacteria (Pseudomonas aeruginosa) from unbound multiwall carbon nanotubes (MWCNTs) and MWCNT agglomerates using sucrose density gradient centrifugation. We demonstrate separation of protozoa (Tetrahymena thermophila) from MWCNTs, bacterial agglomerates, and protozoan fecal pellets by centrifugation in an iodixanol solution. The presence of MWCNTs in the density gradients after centrifugation was determined by quantification of 14C-labeled MWCNTs; the recovery of microbes from the density gradient media was confirmed by optical microscopy. Protozoan intracellular contents of MWCNTs and of bacteria were also unaffected by the designed separation process. The optimized methods contribute to improved efficiency and accuracy in quantifying MWCNT association with bacteria and MWCNT accumulation in protozoan cells, thus supporting improved assessment of CNT bioaccumulation. PMID:27917301

  7. Phase-mixing self-injection into wakefield acceleration structure driven in a rising density gradient

    Science.gov (United States)

    Sahai, Aakash

    2015-11-01

    We model the phase-mixing self-injection of electrons into the plasma-wakefield acceleration structures driven in a longitudinally rising density gradient. In several laser-plasma acceleration experiments a long tail of accelerated electrons of different energies is experimentally observed. Self-injection is the process where some of the plasma electrons lose coherence with the wave due to non-linearities. The non-linearity is inherently and intentionally induced in the plasma oscillations due to the variation of the restoring force along the rising density gradient. These electrons then get trapped in and propagate with the accelerating phase of the plasma-wave. The onset of trapping is shown to scale with the gradient of the rising density and the amplitude of oscillations using the phase-mixing model. We computationally verify the phase-mixing model in planar geometry using PIC codes. The trapping of electrons in cylindrical electron plasma oscillations in the non-linear regime is verified with scaling similar to the planar geometry phase-mixing model. A full theory of longitudinal phase-mixing of radial oscillations is currently underway. The importance of this work for laser-plasma acceleration lies in consistently accelerating just the desired mono-energetic bunch. Work supported by the US Department of Energy under DE-SC0010012 and the National Science Foundation under NSF-PHY-0936278. Done...processed 1928 records...14:16:38

  8. A spatially resolved nucleic acid biochip based on a gradient of density of immobilized probe oligonucleotide

    Energy Technology Data Exchange (ETDEWEB)

    Park, Sung Han [Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto at Mississauga, 3359 Mississauga Road North, Mississauga, Ontario, L5L 1C6 (Canada); Krull, Ulrich [Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto at Mississauga, 3359 Mississauga Road North, Mississauga, Ontario, L5L 1C6 (Canada)]. E-mail: ukrull@utm.utoronto.ca

    2006-04-06

    The potential for a new biochip design based on a continuous gradient of density of immobilized single-stranded DNA oligonucleotide probes (ssDNA) is explored. This gradient resolved information platform (GRIP) can provide sequence identification based on the spatial location and extent of hybridization by a target sequence. Surfaces based on indium-tin oxide (ITO) on glass were first functionalized by 3-aminopropyltriethoxysilane (APTES) followed by attachment of glutaraldehyde, prior to immobilization of oligonucleotide probe that was terminated with amine. The use of Cy{sub 3} and Cy{sub 5} dye-labelled ssDNA probes and targets allowed estimation of density and correlation of the location of binding of labelled targets. Probe molecules of 20 mer lengths were loaded to produce density gradients in the range of 1.0-200 ng/mm{sup 2}. The biochips could resolve a mixture of fully complementary five base-pair mismatched targets by the location of binding on the surface. Thermal control provided additional selectivity. Thermal cycling and washing provided for regeneration of the surface, and the fluorescence intensities showed no deterioration in at least five cycles of hybridization reactions.

  9. The influence of near-wall density and viscosity gradients on turbulence in channel flows

    CERN Document Server

    Patel, Ashish; Pecnik, Rene

    2016-01-01

    The influence of near-wall density and viscosity gradients on near-wall turbulence in a channel are studied by means of Direct Numerical Simulation (DNS) of the low-Mach number approximation of the Navier--Stokes equations. Different constitutive relations for density and viscosity as a function of temperature are used in order to mimic a wide range of fluid behaviours and to develop a generalised framework for studying turbulence modulations in variable property flows. Instead of scaling the velocity solely based on local density, as done for the van Driest transformation, we derive an extension of the scaling that is based on gradients of the semi-local Reynolds number $Re_\\tau^*$. This extension of the van Driest transformation is able to collapse velocity profiles for flows with near-wall property gradients as a function of the semi-local wall coordinate. However, flow quantities like mixing length, turbulence anisotropy and turbulent vorticity fluctuations do not show a universal scaling very close to th...

  10. Isolation of human salivary extracellular vesicles by iodixanol density gradient ultracentrifugation and their characterizations

    Directory of Open Access Journals (Sweden)

    Kazuya Iwai

    2016-05-01

    Full Text Available Diagnostic methods that focus on the extracellular vesicles (EVs present in saliva have been attracting great attention because of their non-invasiveness. EVs contain biomolecules such as proteins, messenger RNA (mRNA and microRNA (miRNA, which originate from cells that release EVs, making them an ideal source for liquid biopsy. Although there have been many reports on density-based fractionation of EVs from blood and urine, the number of reports on EVs from saliva has been limited, most probably because of the difficulties in separating EVs from viscous saliva using density gradient centrifugation. This article establishes a protocol for the isolation of EVs from human saliva using density gradient centrifugation. The fractionated salivary EVs were characterized by atomic force microscopy, western blot and reverse transcription polymerase chain reaction. The results indicate that salivary EVs have a smaller diameter (47.8±12.3 nm and higher density (1.11 g/ml than EVs isolated from conditioned cell media (74.0±23.5 nm and 1.06 g/ml, respectively. Additionally, to improve the throughput of density-based fractionation of EVs, the original protocol was further modified by using a fixed angle rotor instead of a swinging rotor. It was also confirmed that several miRNAs were expressed strongly in the EV-marker-expressing fractions.

  11. Isolation of human salivary extracellular vesicles by iodixanol density gradient ultracentrifugation and their characterizations

    Science.gov (United States)

    Iwai, Kazuya; Minamisawa, Tamiko; Suga, Kanako; Yajima, Yasutomo; Shiba, Kiyotaka

    2016-01-01

    Diagnostic methods that focus on the extracellular vesicles (EVs) present in saliva have been attracting great attention because of their non-invasiveness. EVs contain biomolecules such as proteins, messenger RNA (mRNA) and microRNA (miRNA), which originate from cells that release EVs, making them an ideal source for liquid biopsy. Although there have been many reports on density-based fractionation of EVs from blood and urine, the number of reports on EVs from saliva has been limited, most probably because of the difficulties in separating EVs from viscous saliva using density gradient centrifugation. This article establishes a protocol for the isolation of EVs from human saliva using density gradient centrifugation. The fractionated salivary EVs were characterized by atomic force microscopy, western blot and reverse transcription polymerase chain reaction. The results indicate that salivary EVs have a smaller diameter (47.8±12.3 nm) and higher density (1.11 g/ml) than EVs isolated from conditioned cell media (74.0±23.5 nm and 1.06 g/ml, respectively). Additionally, to improve the throughput of density-based fractionation of EVs, the original protocol was further modified by using a fixed angle rotor instead of a swinging rotor. It was also confirmed that several miRNAs were expressed strongly in the EV-marker-expressing fractions. PMID:27193612

  12. The effect of longitudinal density gradient on electron plasma wake field acceleration

    CERN Document Server

    Tsiklauri, David

    2016-01-01

    3-, 2- and 1-dimensional, particle-in-cell, fully electromagnetic simulations of electron plasma wake field acceleration in the blow out regime are presented. Earlier results are extended by (i) studying the effect of longitudinal density gradient; (ii) avoiding use of co-moving simulation box; (iii) inclusion of ion motion; and (iv) studying fully electromagnetic plasma wake fields. It is established that injecting driving and trailing electron bunches into a positive density gradient of ten-fold increasing density over 10 cm long Lithium vapor plasma, results in spatially more compact and three times larger, compared to the uniform density case, electric fields (-6.4 x 10^{10} V/m), leading to acceleration of the trailing bunch up to 24.4 GeV (starting from initial 20.4 GeV), with an energy transfer efficiencies from leading to trailing bunch of 75 percent. In the uniform density case -2.5 x 10^{10} V/m wake is created leading to acceleration of the trailing bunch up to 22.4 GeV, with an energy transfer eff...

  13. Current Density Functional Theory Using Meta-Generalized Gradient Exchange-Correlation Functionals.

    Science.gov (United States)

    Furness, James W; Verbeke, Joachim; Tellgren, Erik I; Stopkowicz, Stella; Ekström, Ulf; Helgaker, Trygve; Teale, Andrew M

    2015-09-08

    We present the self-consistent implementation of current-dependent (hybrid) meta-generalized gradient approximation (mGGA) density functionals using London atomic orbitals. A previously proposed generalized kinetic energy density is utilized to implement mGGAs in the framework of Kohn-Sham current density functional theory (KS-CDFT). A unique feature of the nonperturbative implementation of these functionals is the ability to seamlessly explore a wide range of magnetic fields up to 1 au (∼235 kT) in strength. CDFT functionals based on the TPSS and B98 forms are investigated, and their performance is assessed by comparison with accurate coupled-cluster singles, doubles, and perturbative triples (CCSD(T)) data. In the weak field regime, magnetic properties such as magnetizabilities and nuclear magnetic resonance shielding constants show modest but systematic improvements over generalized gradient approximations (GGA). However, in the strong field regime, the mGGA-based forms lead to a significantly improved description of the recently proposed perpendicular paramagnetic bonding mechanism, comparing well with CCSD(T) data. In contrast to functionals based on the vorticity, these forms are found to be numerically stable, and their accuracy at high field suggests that the extension of mGGAs to CDFT via the generalized kinetic energy density should provide a useful starting point for further development of CDFT approximations.

  14. Les écoulements par RMN à gradient pulsé Pulsed Gradient Nmr Techniques for Studying Flows

    Directory of Open Access Journals (Sweden)

    Lebon L.

    2006-12-01

    Full Text Available Nous présentons ici les techniques de RMN à gradient pulsé qui permettent d'étudier les écoulements multiphasiques en canalisation ou en milieu poreux. Les principaux avantages sont de pouvoir travailler sur des milieux non transparents et d'accéder à des échelles de longueurs faibles. On montre qu'il est possible d'obtenir des informations locales sur l'écoulement, telles que le profil de vitesse et ses fluctuations dans les écoulements diphasiques, ou les cartes de distribution des probabilités de déplacement dans des échantillons poreux hétérogènes. Pulsed gradient NMR techniques are presented here. They allow the study of multiphase flow in pipes as well as porous media. The main advantages are the possibilities of studying non transparent media at small length scales. We show that it is possible to obtain local information on the fluid flow, such as velocity profiles in two phase systems, or maps of distribution of displacement probabilities in heterogeneous porous media.

  15. Noninvasive prenatal diagnosis. Use of density gradient centrifugation, magnetically activated cell sorting and in situ hybridization

    DEFF Research Database (Denmark)

    Campagnoli, C; Multhaupt, H A; Ludomirski, A;

    1997-01-01

    cells recovered did not differ. Seven of seven male pregnancies were correctly identified. One case of trisomy 21 was detected. CONCLUSION: The in situ hybridization analysis of fetal nucleated erythrocytes isolated from maternal blood using single density gradient centrifugation, anti-CD71/anti...... of the isolated cells were subjected to in situ hybridization with specific DNA probes for the Y chromosome and chromosome 21 to confirm the fetal origin. RESULTS: After MiniMACS the enrichment factors for the CD71/GPA- and CD36/GPA-positive cells from maternal blood were similar, and the percentages of fetal...

  16. Density functional theory optimized basis sets for gradient corrected functionals: 3d transition metal systems.

    Science.gov (United States)

    Calaminici, Patrizia; Janetzko, Florian; Köster, Andreas M; Mejia-Olvera, Roberto; Zuniga-Gutierrez, Bernardo

    2007-01-28

    Density functional theory optimized basis sets for gradient corrected functionals for 3d transition metal atoms are presented. Double zeta valence polarization and triple zeta valence polarization basis sets are optimized with the PW86 functional. The performance of the newly optimized basis sets is tested in atomic and molecular calculations. Excitation energies of 3d transition metal atoms, as well as electronic configurations, structural parameters, dissociation energies, and harmonic vibrational frequencies of a large number of molecules containing 3d transition metal elements, are presented. The obtained results are compared with available experimental data as well as with other theoretical data from the literature.

  17. The formulation and implementation of analytic energy gradients for periodic density functional calculations with STO/NAO Bloch basis set

    NARCIS (Netherlands)

    Kadantsev, Eugene S.; Klooster, Rob; De Boeij, Paul L.; Ziegler, Tom

    2007-01-01

    Analytic energy gradients with respect to atomic coordinates for systems with translational invariance are formulated within the framework of Kohn-Sham Density Functional Theory. The energy gradients are implemented in the BAND program for periodic DFT calculations which directly employs a Bloch bas

  18. Minimax current density gradient coils: analysis of coil performance and heating.

    Science.gov (United States)

    Poole, Michael S; While, Peter T; Lopez, Hector Sanchez; Crozier, Stuart

    2012-08-01

    Standard gradient coils are designed by minimizing the inductance or resistance for an acceptable level of gradient field nonlinearity. Recently, a new method was proposed to minimize the maximum value of the current density in a coil additionally. The stated aim of that method was to increase the minimum wire spacing and to reduce the peak temperature in a coil for fixed efficiency. These claims are tested in this study with experimental measurements of magnetic field and temperature as well as simulations of the performance of many coils. Experimental results show a 90% increase in minimum wire spacing and 40% reduction in peak temperature for equal coil efficiency and field linearity. Simulations of many more coils indicate increase in minimum wire spacing of between 50 and 340% for the coils studied here. This method is shown to be able to increase coil efficiency when constrained by minimum wire spacing rather than switching times or total power dissipation. This increase in efficiency could be used to increase gradient strength, duty cycle, or buildability.

  19. Affinity-mediated sorting order reversal of single-walled carbon nanotubes in density gradient ultracentrifugation

    Science.gov (United States)

    Jang, Myungsu; Kim, Somin; Jeong, Haneul; Ju, Sang-Yong

    2016-10-01

    Sorted single-walled carbon nanotubes (SWNTs) are of paramount importance for their utilization in high-end optoelectronic applications. Sodium cholate (SC)-based density gradient ultracentrifugation (DGU) has been instrumental in isolating small diameter (d t) SWNTs. Here, we show that SWNTs wrapped by flavin mononucleotide (FMN) as a dispersing agent are sorted in DGU, and show sorting order reversal behavior, departing from prototypical SC-SWNT trends. Larger d t SWNTs are sorted in lower density (ρ), and buoyant ρ distribution of FMN-SWNT ranges from 1.15-1.25 g cm-3. Such a nanotube layering pattern originates from both the binding affinity between FMN and SWNT and the less-susceptible hydrated volume of remote phosphate sidechains of FMN according to nanotube d t change.

  20. Vole preference of bilberry along gradients of simulated moose density and site productivity.

    Science.gov (United States)

    Pedersen, Simen; Andreassen, Harry P; Persson, Inga-Lill; Julkunen-Tiitto, Riitta; Danell, Kjell; Skarpe, Christina

    2011-12-01

    Browsing by large herbivores might either increase or decrease preference for the plant by other herbivores, depending on the plant response. Using a cafeteria test, we studied the preference by root voles (Microtus oeconomus [Pallas, 1776]) for bilberry (Vaccinium myrtillus L.) previously subjected to 4 levels of simulated moose (Alces alces [Linnaeus, 1758]) density. The different levels of moose density were simulated at population densities relevant for Fennoscandian conditions, in exclosures situated along a site productivity gradient. We expected: (i) voles to prefer bilberry from high productivity sites over low productivity sites; (ii) voles to prefer browsed bilberry, if plants allocate resources to compensatory growth or to avoid browsed bilberry if plants allocate resources to defense; (iii) these effects to increase with increasing simulated moose density; and (iv) the concentration of plant chemicals and the plant morphology to explain vole preference. Specifically, we predicted that voles would prefer: (i) plants with high nitrogen content; (ii) plants with low content of defensive substances; and (iii) tall plants with long shoots. Voles preferred bilberry from the high productivity sites compared to the low productivity sites. We also found an interaction between site productivity and simulated moose density, where voles preferred unbrowsed plants at low productivity sites and intermediate levels of browsing at high productivity sites. There was no effect of plant chemistry or morphology on vole preference. We conclude that moose browsing impacts the food preference of voles. With the current high densities of moose in Fennoscandia, this could potentially influence vole food selection and population dynamics over large geographical areas.

  1. Reproducible isolation of type II pneumocytes from fetal and adult rat lung using nycodenz density gradients.

    Science.gov (United States)

    Viscardi, R M; Ullsperger, S; Resau, J H

    1992-01-01

    Isolating fresh, relatively pure type II pneumocytes from the lung, particularly of fetal origin, is a difficult process. Separation by buoyant density gradient centrifugation has been used successfully to isolate adult type II cells. There is concern, however, that Percoll, a gradient medium that is commonly used for type II cell isolation, may be toxic to cells. We evaluated a new gradient medium, Nycodenz, that is (1) a true solution, (2) transparent, (3) not metabolized by cells, and (4) nontoxic to cells. Type II pneumocytes were isolated from 19- and 21-day gestation fetal and adult rat lung by elastase digestion and separated on preformed isotonic Nycodenz gradients (2 mL each of 27.6, 20.7, 13.8, and 4.6 (w/v) solutions). Type II pneumocytes were recovered from the density range 1.057-1.061 and identified by binding of FITC-conjugated and gold-complexed Maclura pomifera lectin. Cells derived from 19-day fetal lung contained abundant glycogen and reacted with a monoclonal antibody to the cytokeratins 8 and 18, which are markers of the fetal type II cell. Adult type II cells reacted with antibodies to cytokeratins 8, 18, and 19. Type II cell purity was 79.7 +/- 2.4%, 83.8 +/- 2.8%, and 82.6 +/- 1.8% (means +/- SEM) for 19- and 21-day gestation fetal and adult lung preparations, respectively. Cell viability was greater than 95%. The final cell yield for adult preparations was 17.8 +/- 2.7 x 10(6)/rat (means +/- SEM). To determine if the freshly isolated type II pneumocytes were functionally active, the incorporation of [3H]choline into phosphatidylcholine was measured. The percent saturation of phosphatidylcholine was high for both populations of freshly isolated cells. However, adult type II pneumocytes incorporated [3H]choline into phosphatidylcholine more rapidly than 21-day gestation fetal cells (5.97 x 10(-3) dpm/10(6) cells/h vs. 0.32 x 10(-3) dpm/10(6) cells/h, P less than .005). We have demonstrated that, using the Nycodenz isolation method, it is

  2. Comparison of bone density measurement techniques: DXA and Archimedes' principle.

    Science.gov (United States)

    Keenan, M J; Hegsted, M; Jones, K L; Delany, J P; Kime, J C; Melancon, L E; Tulley, R T; Hong, K D

    1997-11-01

    The standard method for determination of density (g/cm3) of bones from small animals has been the application of Archimedes' principle. A recent development has been software for the determination of "density" (g/cm2) of small animal bones with dual-energy X-ray absorptiometry (DXA). We compared Archimedes' principle and DXA (Hologic QDR-2000) in the measurement of the densities of whole and hollowed femurs of 5- to 6-month-old retired female breeder rats. In an attempt to ensure detectable treatment differences, rats were used from a low-vitamin D Holtzman and a supplemental-vitamin D Sprague-Dawley colony. Whole femur densities were higher for supplemental-vitamin D colony rats than for low vitamin D rats using both techniques (Archimedes' principle, p Archimedes' principle than for DXA. Other variables such as femur ash weight and calcium content were also highly correlated to densities with both techniques. Hollowed femur density values were higher than whole femur values with Archimedes' principle but lower with DXA. Colony effects for hollowed femur densities were diminished with Archimedes' principle (p < 0.03) and eliminated with DXA (p < 0.53). Investigation of whole bones is more biologically relevant, and both techniques were effective in detecting differences between whole femurs from low-vitamin D and supplemental-vitamin D colony rats.

  3. Sea surface density gradients in the Nordic Seas during the Holocene as revealed by paired microfossil and isotope proxies

    DEFF Research Database (Denmark)

    Van Nieuwenhove, Nicolas; Hillaire-Marcel, Claude; Bauch, Henning A.

    2016-01-01

    We attempt to assess the Holocene surface-subsurface seawater density gradient on millennial time-scale based on the reconstruction of potential density (σθ) by combining data from dinoflagellate cyst assemblages and planktic foraminiferal (Neogloboquadrina pachyderma (s)) stable oxygen isotopes (δ...

  4. Controls on stand transpiration and soil water utilization along a tree density gradient in a Neotropical savanna

    Science.gov (United States)

    Sandra J. Bucci; Fabian G. Scholz; Guillermo Goldstein; William A. Hoffmann; Frederick C. Meinzer; Augusto C. Franco; Thomas Giambelluca; Fernando Miralles-Wilhelm

    2008-01-01

    Environmental controls of stand-level tree transpiration (E) and seasonal patterns of soil water utilization were studied in five central Brazilian savanna (Cerrado) sites differing in tree density. Tree density of Cerrado vegetation in the study area consistently changes along topographic gradients from ~1,000 trees ha-1 in open savannas (campo...

  5. Emergence flux declines disproportionately to larval density along a stream metals gradient.

    Science.gov (United States)

    Schmidt, Travis S; Kraus, Johanna M; Walters, David M; Wanty, Richard B

    2013-08-01

    Effects of contaminants on adult aquatic insect emergence are less well understood than effects on insect larvae. We compared responses of larval density and adult emergence along a metal contamination gradient. Nonlinear threshold responses were generally observed for larvae and emergers. Larval densities decreased significantly at low metal concentrations but precipitously at concentrations of metal mixtures above aquatic life criteria (cumulative criterion accumulation ratio (CCAR) ≥ 1). In contrast, adult emergence declined precipitously at low metal concentrations (CCAR ≤ 1), followed by a modest decline above this threshold. Adult emergence was a more sensitive indicator of the effect of low metals concentrations on aquatic insect communities compared to larvae, presumably because emergence is limited by a combination of larval survival and other factors limiting successful emergence. Thus effects of exposure to larvae are not manifest until later in life (during metamorphosis and emergence). This loss in emergence reduces prey subsidies to riparian communities at concentrations considered safe for aquatic life. Our results also challenge the widely held assumption that adult emergence is a constant proportion of larval densities in all streams.

  6. Current Density-Functional Theory using meta-Generalized Gradient Exchange--Correlation Functionals

    CERN Document Server

    Furness, James W; Tellgren, Erik I; Stopkowicz, Stella; Ekström, Ulf; Helgaker, Trygve; Teale, Andrew M

    2015-01-01

    We present the self-consistent implementation of current-dependent (hybrid) meta generalized gradient approximation (mGGA) density functionals using London atomic orbitals. A previously proposed generalized kinetic energy density is utilized to implement mGGAs in the framework of Kohn--Sham current density-functional theory (KS-CDFT). A unique feature of the non-perturbative implementation of these functionals is the ability to seamlessly explore a wide range of magnetic fields up to 1 a.u. ($\\sim 235000$T) in strength. CDFT functionals based on the TPSS and B98 forms are investigated and their performance is assessed by comparison with accurate CCSD(T) data. In the weak field regime magnetic properties such as magnetizabilities and NMR shielding constants show modest but systematic improvements over GGA functionals. However, in strong field regime the mGGA based forms lead to a significantly improved description of the recently proposed perpendicular paramagnetic bonding mechanism, comparing well with CCSD(T...

  7. Strain energy density gradients in bone marrow predict osteoblast and osteoclast activity: a finite element study.

    Science.gov (United States)

    Webster, Duncan; Schulte, Friederike A; Lambers, Floor M; Kuhn, Gisela; Müller, Ralph

    2015-03-18

    Huiskes et al. hypothesized that mechanical strains sensed by osteocytes residing in trabecular bone dictate the magnitude of load-induced bone formation. More recently, the mechanical environment in bone marrow has also been implicated in bone׳s response to mechanical stimulation. In this study, we hypothesize that trabecular load-induced bone formation can be predicted by mechanical signals derived from an integrative µFE model, incorporating a description of both the bone and marrow phase. Using the mouse tail loading model in combination with in vivo micro-computed tomography (µCT) we tracked load induced changes in the sixth caudal vertebrae of C57BL/6 mice to quantify the amount of newly mineralized and eroded bone volumes. To identify the mechanical signals responsible for adaptation, local morphometric changes were compared to micro-finite element (µFE) models of vertebrae prior to loading. The mechanical parameters calculated were strain energy density (SED) on trabeculae at bone forming and resorbing surfaces, SED in the marrow at the boundary between bone forming and resorbing surfaces, along with SED in the trabecular bone and marrow volumes. The gradients of each parameter were also calculated. Simple regression analysis showed mean SED gradients in the trabecular bone matrix to significantly correlate with newly mineralized and eroded bone volumes R(2)=0.57 and 0.41, respectively, pbone marrow plays a significant role in determining osteoblast and osteoclast activity.

  8. Two-dimensional radial acquisition technique with density adaption in sodium MRI.

    Science.gov (United States)

    Konstandin, Simon; Nagel, Armin M; Heiler, Patrick M; Schad, Lothar R

    2011-04-01

    Conventional 2D radial projections suffer from losses in signal-to-noise ratio efficiency because of the nonuniform k-space sampling. In this study, a 2D projection reconstruction method with variable gradient amplitudes is presented to cover the k-space uniformly. The gradient is designed to keep the average sampling density constant. By this, signal-to-noise ratio is increased, and the linear form of the radial trajectory is kept. The simple gradient design and low hardware requirements in respect of slew rate allow an easy implementation at MR scanners. Measurements with the density-adapted 2D radial trajectory were compared with the conventional projection reconstruction method. It is demonstrated that the density-adapted 2D radial trajectory technique provides higher signal-to-noise ratio (up to 28% in brain tissue), less blurring, and fewer artifacts in the presence of magnetic field inhomogeneities than imaging with the conventional 2D radial trajectory scheme. The presented sequence is well-suited for electrocardiographically gated sodium heart MRI and other applications with short relaxation times. Copyright © 2010 Wiley-Liss, Inc.

  9. DIAGNOSTIC VALUE OF DENSITY GRADIENT CENTRIFUGATION FOR EXFOLIATIVE TUMOR CELLS IN MALIGNANT PLEURAL EFFUSIONS

    Institute of Scientific and Technical Information of China (English)

    郭胤仕; 朱任之

    2004-01-01

    Objective To find out a specific method for diagnosis of malignant pleural effusions( MPEs )with higher sensitivity and practicality. Methods The diagnosis of MPEs were made using density gradient centrifugation ( DGC ) , smear cytologic examination (SCE) and pleural needle biopsy (PNB). Comparisons between these results and those of benign pleural effusions were also made. Results The positive rates of DGC,SCE and PNB for diagnosing MPEs were 94. 3% ,62.9% and 44.6% , respectively, and the positive rate of SCE combined with PNB for diagnosing MPEs was 73.2 %. The positive rate of the exfoliative tumor cells ( ETCs ) by DGC was much higher than that of SCE or/and PNB with no false-positive. Conclusion The ETCs isolated by DGC from the MPEs is quite specific for the diagnosis of malignant tumors with higher sensitivity and practicality in clinico-pathological practice.

  10. Separation of arginase isoforms by capillary zone electrophoresis and isoelectric focusing in density gradient column.

    Science.gov (United States)

    Pedrosa, M M; Legaz, M E

    1995-04-01

    Four major arginase isoforms, I, II, III and IV, have been detected in Evernia prunastri thallus. They differ in terms of both physical and biochemical properties. The isoelectric point (pI) of these proteins has been determined by both isoelectric focusing in density gradient column and high-performance capillary electrophoresis (HPCE). Isoelectric focusing revealed charge microheterogeneity for isoforms II and IV whereas arginases I and II had the same pI value of 5.8. HPCE separation confirmed this charge microheterogeneity for isoform IV but not for isoform III, and provided evidence of microheterogeneity for isoforms I and II. The effect of various electrolyte buffers and running conditions on the HPCE separation of arginase isoform were investigated. Addition of 0.5 mM spermidine (SPD) to the running buffer reduced the electroosmotic flow (EOF) and permitted discriminating between the native proteins and protein fragments.

  11. A Novel Microcharacterization Technique in the Measurement of Strain and Orientation Gradient in Advanced Materials

    Science.gov (United States)

    Garmestai, H.; Harris, K.; Lourenco, L.

    1997-01-01

    Representation of morphology and evolution of the microstructure during processing and their relation to properties requires proper experimental techniques. Residual strains, lattice distortion, and texture (micro-texture) at the interface and the matrix of a layered structure or a functionally gradient material and their variation are among parameters important in materials characterization but hard to measure with present experimental techniques. Current techniques available to measure changes in interred material parameters (residual stress, micro-texture, microplasticity) produce results which are either qualitative or unreliable. This problem becomes even more complicated in the case of a temperature variation. These parameters affect many of the mechanical properties of advanced materials including stress-strain relation, ductility, creep, and fatigue. A review of some novel experimental techniques using recent advances in electron microscopy is presented here to measure internal stress, (micro)texture, interracial strength and (sub)grain formation and realignment. Two of these techniques are combined in the chamber of an Environmental Scanning Electron Microscope to measure strain and orientation gradients in advanced materials. These techniques which include Backscattered Kikuchi Diffractometry (BKD) and Microscopic Strain Field Analysis are used to characterize metallic and intermetallic matrix composites and superplastic materials. These techniques are compared with the more conventional x-ray diffraction and indentation techniques.

  12. Self-injection by trapping of plasma electrons oscillating in rising density gradient at the vacuum-plasma interface

    CERN Document Server

    Sahai, Aakash A; Muggli, Patric

    2014-01-01

    We model the trapping of plasma $e^-$ within the density structures excited by a propagating energy source ($\\beta_{S}\\simeq1$) in a rising plasma density gradient. Rising density gradient leads to spatially contiguous coupled up-chirped plasmons ($d{\\omega^2_{pe}(x)}/{dx}>0$). Therefore phase mixing between plasmons can lead to trapping until the plasmon field is high enough such that $e^-$ trajectories returning towards a longer wavelength see a trapping potential. Rising plasma density gradients are ubiquitous for confining the plasma within sources at the vacuum-plasma interfaces. Therefore trapping of plasma-$e^-$ in a rising ramp is important for acceleration diagnostics and to understand the energy dissipation from the excited plasmon train \\cite{LTE-2013}. Down-ramp in density \\cite{density-transition-2001} has been used for plasma-$e^-$ trapping within the first bucket behind the driver. Here, in rising density gradient the trapping does not occur in the first plasmon bucket but in subsequent plasmon...

  13. Gradient chromatofocusing-mass spectrometry: a new technique in protein analysis.

    Science.gov (United States)

    Shan, Lian; Hribar, James A; Zhou, Xiang; Anderson, David J

    2008-08-01

    A new analytical technique, gradient chromatofocusing-mass spectrometry (gCF-MS), was developed employing ion-exchange high-performance liquid chromatography (HPLC) interfaced to an electrospray-quadrupole mass spectrometer in the determination of proteins. There have been few reports, if any, of a HPLC-MS technique for proteins in which the ion-exchange column is directly interfaced to the mass spectrometer. The employment of a linear pH gradient elution scheme directly interfaced to mass spectrometry is also unique in the present work. The technique was demonstrated by the separation of six proteins (carbonic anhydrase II, enolase, beta-lactoglobulin A, lactoglobulin B, soybean trypsin inhibitor, and amyloglucosidase) employing a descending linear pH gradient from pH 9 to 2.6 on a 50 mm x 2.1 mm DEAE HPLC column using volatile buffer components. A signal enhancement solution consisting of 8% formic acid in acetonitrile was pumped post-column and was mixed 1:1 with column effluent and then directed on-line into the mass spectrometer. Molecular masses of the proteins were determined within +/-0.010% to 0.033% (+/-100 to 330 ppm) with peak height total ion current detection limits of 4 to 78 pmol of injected amounts (S/N = 3). This technique is applicable to the analysis of proteins and other charged molecules.

  14. Phase-mixing self-injection into plasma-wakefield acceleration structures driven in a rising density gradient

    Science.gov (United States)

    Sahai, Aakash Ajit

    We model the phase-mixing self-injection of electrons into plasma-wakefield acceleration structures driven in a longitudinally rising density gradient. Self-injection is the process where some of the plasma electrons lose coherence with the wave due to non-linearities. The non-linearity is inherently and intentionally induced in the plasma oscillations due to the variation of the restoring force along the rising density gradient. These electrons then get trapped in and propagate with the accelerating phase of the plasma-wave. The electron oscillations driven by matched energy-sources are shown to get trapped in the wakefields similar in scaling to the phase-mixing of free oscillations. The onset of trapping is shown to scale with the gradient of rising density and the amplitude of oscillations. The planar longitudinal electron oscillations undergo trajectory crossing above a threshold amplitude or in a density inhomogeneity leading to phase-mixing and trapping of the oscillating electrons to a phase of the wave. In this thesis, we analyze the scaling of the phase-mixing based trapping of electron oscillations, independent of a threshold, in planar geometry driven by an electron beam in a rising density gradient. The cylindrical and spherical geometry electron oscillations undergo phase-mixing irrespective of the amplitude of oscillations. Here, driven radial electron oscillations in cylindrical geometry are shown to undergo phase-mixing leading to trapping of the plasma electrons in a longitudinally rising density gradient. We also present preliminary scaling results of phase-mixing based trapping of radially oscillating electrons in a rising density gradient.

  15. Role of Density Gradient Driven Trapped Electron Modes in the H-Mode Inner Core with Electron Heating

    Science.gov (United States)

    Ernst, D.

    2015-11-01

    We present new experiments and nonlinear gyrokinetic simulations showing that density gradient driven TEM (DGTEM) turbulence dominates the inner core of H-Mode plasmas during strong electron heating. Thus α-heating may degrade inner core confinement in H-Mode plasmas with moderate density peaking. These DIII-D low torque quiescent H-mode experiments were designed to study DGTEM turbulence. Gyrokinetic simulations using GYRO (and GENE) closely match not only particle, energy, and momentum fluxes, but also density fluctuation spectra, with and without ECH. Adding 3.4 MW ECH doubles Te /Ti from 0.5 to 1.0, which halves the linear TEM critical density gradient, locally flattening the density profile. Density fluctuations from Doppler backscattering (DBS) intensify near ρ = 0.3 during ECH, displaying a band of coherent fluctuations with adjacent toroidal mode numbers. GYRO closely reproduces the DBS spectrum and its change in shape and intensity with ECH, identifying these as coherent TEMs. Prior to ECH, parallel flow shear lowers the effective nonlinear DGTEM critical density gradient 50%, but is negligible during ECH, when transport displays extreme stiffness in the density gradient. GS2 predictions show the DGTEM can be suppressed, to avoid degradation with electron heating, by broadening the current density profile to attain q0 >qmin > 1 . A related experiment in the same regime varied the electron temperature gradient in the outer half-radius (ρ ~ 0 . 65) using ECH, revealing spatially coherent 2D mode structures in the Te fluctuations measured by ECE imaging. Fourier analysis with modulated ECH finds a threshold in Te profile stiffness. Supported by the US DOE under DE-FC02-08ER54966 and DE-FC02-04ER54698.

  16. The transport phenomena during the growth of ZnTe crystal by the temperature gradient solution growth technique

    Science.gov (United States)

    Yin, Liying; Jie, Wanqi; Wang, Tao; Zhou, Boru; Yang, Fan

    2017-03-01

    A numerical model is developed to simulate the temperature field, the thermosolutal convection, the solute segregation and the growth interface morphology during the growth of ZnTe crystal from Te rich solution by the temperature gradient solution growth (TGSG) technique. Effects of the temperature gradient on the transport phenomena, the growth interface morphology and the growth rate are examined. The influences of the latent heat and the thermal conductivity of ZnTe crystal on the transport phenomena and the growth interface are also discussed. We find that the mass transfer of ZnTe in the solution is very slow because of the low diffusion coefficient and the lack of mixing in the lower part of the solution. During the growth, dilute solution with high density and low growth temperature accumulates in the central region of the growth interface, making the growth interface change into two distinct parts. The inner part is very concave, while the outer part is relatively flat. Growth conditions in front of the two parts of the growth interface are different. The crystalline quality of the inner part of the ingot is predicted to be worse than that of the outer part. High temperature gradient can significantly increase the growth rate, and avoid the diffusion controlled growth to some extent.

  17. Versatile van der Waals Density Functional Based on a Meta-Generalized Gradient Approximation

    Directory of Open Access Journals (Sweden)

    Haowei Peng

    2016-10-01

    Full Text Available A “best-of-both-worlds” van der Waals (vdW density functional is constructed, seamlessly supplementing the strongly constrained and appropriately normed (SCAN meta-generalized gradient approximation for short- and intermediate-range interactions with the long-range vdW interaction from rVV10, the revised Vydrov–van Voorhis nonlocal correlation functional. The resultant SCAN+rVV10 is the only vdW density functional to date that yields excellent interlayer binding energies and spacings, as well as intralayer lattice constants in 28 layered materials. Its versatility for various kinds of bonding is further demonstrated by its good performance for 22 interactions between molecules; the cohesive energies and lattice constants of 50 solids; the adsorption energy and distance of a benzene molecule on coinage-metal surfaces; the binding energy curves for graphene on Cu(111, Ni(111, and Co(0001 surfaces; and the rare-gas solids. We argue that a good semilocal approximation should (as SCAN does capture the intermediate-range vdW through its exchange term. We have found an effective range of the vdW interaction between 8 and 16 Å for systems considered here, suggesting that this interaction is negligibly small at the larger distances where it reaches its asymptotic power-law decay.

  18. Versatile van der Waals Density Functional Based on a Meta-Generalized Gradient Approximation

    Science.gov (United States)

    Peng, Haowei; Yang, Zeng-Hui; Perdew, John P.; Sun, Jianwei

    2016-10-01

    A "best-of-both-worlds" van der Waals (vdW) density functional is constructed, seamlessly supplementing the strongly constrained and appropriately normed (SCAN) meta-generalized gradient approximation for short- and intermediate-range interactions with the long-range vdW interaction from r VV 10 , the revised Vydrov-van Voorhis nonlocal correlation functional. The resultant SCAN +r VV 10 is the only vdW density functional to date that yields excellent interlayer binding energies and spacings, as well as intralayer lattice constants in 28 layered materials. Its versatility for various kinds of bonding is further demonstrated by its good performance for 22 interactions between molecules; the cohesive energies and lattice constants of 50 solids; the adsorption energy and distance of a benzene molecule on coinage-metal surfaces; the binding energy curves for graphene on Cu(111), Ni(111), and Co(0001) surfaces; and the rare-gas solids. We argue that a good semilocal approximation should (as SCAN does) capture the intermediate-range vdW through its exchange term. We have found an effective range of the vdW interaction between 8 and 16 Å for systems considered here, suggesting that this interaction is negligibly small at the larger distances where it reaches its asymptotic power-law decay.

  19. The effect of electron beam pitch angle and density gradient on solar type III radio bursts

    CERN Document Server

    Pechhacker, Roman

    2012-01-01

    1.5D Particle-In-Cell simulations of a hot, low density electron beam injected into magnetized, maxwellian plasma were used to further explore the alternative non-gyrotropic beam driven electromagnetic emission mechanism, first studied in Tsiklauri (2011). Variation of beam injection angle and background density gradient showed that the emission process is caused by the perpendicular component of the beam injection current, whereas the parallel component only produces Langmuir waves, which play no role in the generation of EM waves in our mechanism. Particular emphasis was put on the case, where the beam is injected perpendicularly to the background magnetic field, as this turned off any electrostatic wave generation along the field and left a purely electromagnetic signal in the perpendicular components. The simulations establish the following key findings: i) Initially waves at a few w_ce/gamma are excited, mode converted and emitted at w_pe ii) The emission intensity along the beam axis is proportional to ...

  20. Analytic energy gradients for the coupled-cluster singles and doubles method with the density-fitting approximation.

    Science.gov (United States)

    Bozkaya, Uğur; Sherrill, C David

    2016-05-07

    An efficient implementation is presented for analytic gradients of the coupled-cluster singles and doubles (CCSD) method with the density-fitting approximation, denoted DF-CCSD. Frozen core terms are also included. When applied to a set of alkanes, the DF-CCSD analytic gradients are significantly accelerated compared to conventional CCSD for larger molecules. The efficiency of our DF-CCSD algorithm arises from the acceleration of several different terms, which are designated as the "gradient terms": computation of particle density matrices (PDMs), generalized Fock-matrix (GFM), solution of the Z-vector equation, formation of the relaxed PDMs and GFM, back-transformation of PDMs and GFM to the atomic orbital (AO) basis, and evaluation of gradients in the AO basis. For the largest member of the alkane set (C10H22), the computational times for the gradient terms (with the cc-pVTZ basis set) are 2582.6 (CCSD) and 310.7 (DF-CCSD) min, respectively, a speed up of more than 8-folds. For gradient related terms, the DF approach avoids the usage of four-index electron repulsion integrals. Based on our previous study [U. Bozkaya, J. Chem. Phys. 141, 124108 (2014)], our formalism completely avoids construction or storage of the 4-index two-particle density matrix (TPDM), using instead 2- and 3-index TPDMs. The DF approach introduces negligible errors for equilibrium bond lengths and harmonic vibrational frequencies.

  1. Gradient vs. approximation design optimization techniques in low-dimensional convex problems

    Science.gov (United States)

    Fedorik, Filip

    2013-10-01

    Design Optimization methods' application in structural designing represents a suitable manner for efficient designs of practical problems. The optimization techniques' implementation into multi-physical softwares permits designers to utilize them in a wide range of engineering problems. These methods are usually based on modified mathematical programming techniques and/or their combinations to improve universality and robustness for various human and technical problems. The presented paper deals with the analysis of optimization methods and tools within the frame of one to three-dimensional strictly convex optimization problems, which represent a component of the Design Optimization module in the Ansys program. The First Order method, based on combination of steepest descent and conjugate gradient method, and Supbproblem Approximation method, which uses approximation of dependent variables' functions, accompanying with facilitation of Random, Sweep, Factorial and Gradient Tools, are analyzed, where in different characteristics of the methods are observed.

  2. Bounded perturbation resilience and superiorization techniques for the projected scaled gradient method

    Science.gov (United States)

    Xu, Hong-Kun

    2017-04-01

    Bounded perturbation resilience and superiorization techniques for the projected scaled gradient (PSG) method are studied under the general Hilbert space setting. Weak convergence results of the (superiorized) PSG method and its relaxed version are proved under the assumption that the errors be summable. It is also shown that the PSG method converges in a sublinear rate and can be accelerated to the convergence rate O≤ft(\\tfrac{1}{{n}2}\\right). Applications to linear inverse problems and split feasibility problems are discussed.

  3. Role of density gradient driven trapped electron mode turbulence in the H-mode inner core with electron heating

    Science.gov (United States)

    Ernst, D. R.; Burrell, K. H.; Guttenfelder, W.; Rhodes, T. L.; Dimits, A. M.; Bravenec, R.; Grierson, B. A.; Holland, C.; Lohr, J.; Marinoni, A.; McKee, G. R.; Petty, C. C.; Rost, J. C.; Schmitz, L.; Wang, G.; Zemedkun, S.; Zeng, L.

    2016-05-01

    A series of DIII-D [J. L. Luxon, Nucl. Fusion 42, 614 (2002)] low torque quiescent H-mode experiments show that density gradient driven trapped electron mode (DGTEM) turbulence dominates the inner core of H-mode plasmas during strong electron cyclotron heating (ECH). Adding 3.4 MW ECH doubles Te/Ti from 0.5 to 1.0, which halves the linear DGTEM critical density gradient, locally reducing density peaking, while transport in all channels displays extreme stiffness in the density gradient. This suggests that fusion α-heating may degrade inner core confinement in H-mode plasmas with moderate density peaking and low collisionality, with equal electron and ion temperatures, key conditions expected in burning plasmas. Gyrokinetic simulations using GYRO [J. Candy and R. E. Waltz, J. Comput. Phys. 186, 545 (2003)] (and GENE [Jenko et al., Phys. Plasmas 7, 1904 (2000)]) closely match not only particle, energy, and momentum fluxes but also density fluctuation spectra from Doppler backscattering (DBS), with and without ECH. Inner core DBS density fluctuations display discrete frequencies with adjacent toroidal mode numbers, which we identify as DGTEMs. GS2 [Dorland et al., Phys. Rev. Lett. 85, 5579 (2000)] predictions show the DGTEM can be suppressed, to avoid degradation with electron heating, by broadening the current density profile to attain q0>qmin>1 .

  4. Sperm fractions obtained following density gradient centrifugation in human ejaculates show differences in sperm DNA longevity

    Institute of Scientific and Technical Information of China (English)

    Jaime Goslvez; Stephen Johnston; Carmen Lpez-Fernndez; Altea Goslbez; Francisca Arroyo; Jose Lus Fernndez; Juan G lvarez

    2014-01-01

    Objective:To investigate the DNA longevity characteristics associated with each resultant fraction following density gradient centrifugation (DGC) in comparison to that of the original neat ejaculated sample. Methods:An aliquot of neat semen (NSS) collected from 7 patients was processed using DGC resulting in 3 fractions;Fraction 1:seminal plasma/40%gradient interface (GI);Fraction 2:40%GI/80%GI;Fraction 3:80%GI/pellet. An aliquot of each fraction and NSS was cryopreserved, thawed and incubated at 37 ℃for 24h;the increase of sperm DNA fragmentation was assessed using the Dyn-Halosperm assay following 0, 3, 6 and 24h of incubation. Results:While there was a significant reduction in the incidence of baseline sperm DNA fragmentation following DGC in Fraction 3, sperm DNA longevity was shown to be higher in the NSS than in any other sub-population following incubation. The highest levels of baseline DNA damage were found in Fractions 1 and 2;these fractions also showed the highest rate DNA fragmentation following incubation, subsequently exhibiting the lowest DNA longevity. Conclusion:1) Unnecessary incubation of spermatozoa prior to artificial insemination or in vitro fertilization, should be avoided, since sperm DNA longevity is significantly reduced after ex vivo sperm handling and 2) Although sperm selection by DCG significantly reduces the baseline levels of SDF of sperm in Fraction 3, sperm DNA longevity in this fraction was ultimately lower following 24 h incubation when compared to sperm recovered from non-centrifuged NSS.

  5. Gradient-based multiobjective optimization using a distance constraint technique and point replacement

    Science.gov (United States)

    Sato, Yuki; Izui, Kazuhiro; Yamada, Takayuki; Nishiwaki, Shinji

    2016-07-01

    This paper proposes techniques to improve the diversity of the searching points during the optimization process in an Aggregative Gradient-based Multiobjective Optimization (AGMO) method, so that well-distributed Pareto solutions are obtained. First to be discussed is a distance constraint technique, applied among searching points in the objective space when updating design variables, that maintains a minimum distance between the points. Next, a scheme is introduced that deals with updated points that violate the distance constraint, by deleting the offending points and introducing new points in areas of the objective space where searching points are sparsely distributed. Finally, the proposed method is applied to example problems to illustrate its effectiveness.

  6. Identification techniques for phenomenological models of hysteresis based on the conjugate gradient method

    Energy Technology Data Exchange (ETDEWEB)

    Andrei, Petru [Electrical and Computer Engineering Department, Florida State Unviersity, Tallahassee, FL 32310 (United States) and Electrical and Computer Engineering Department, Florida A and M Unviersity, Tallahassee, FL 32310 (United States)]. E-mail: pandrei@eng.fsu.edu; Oniciuc, Liviu [Electrical and Computer Engineering Department, Florida State Unviersity, Tallahassee, FL 32310 (United States); Stancu, Alexandru [Faculty of Physics, ' Al. I. Cuza' University, Iasi 700506 (Romania); Stoleriu, Laurentiu [Faculty of Physics, ' Al. I. Cuza' University, Iasi 700506 (Romania)

    2007-09-15

    An identification technique for the parameters of phenomenological models of hysteresis is presented. The basic idea of our technique is to set up a system of equations for the parameters of the model as a function of known quantities on the major or minor hysteresis loops (e.g. coercive force, susceptibilities at various points, remanence), or other magnetization curves. This system of equations can be either over or underspecified and is solved by using the conjugate gradient method. Numerical results related to the identification of parameters in the Energetic, Jiles-Atherton, and Preisach models are presented.

  7. Laser-strophometry high-resolution technique for velocity gradient measurements in fluid flows

    CERN Document Server

    Staude, Wilfried

    2001-01-01

    This book describes techniques that allow the measurement of arbitrary velocity gradient components in fluids with high spatial and temporal resolution, e.g. turbulent fluids. The techniques are based on the properties of scattered laser light. The book gives a detailed and rigorous treatment of the physical and mathematical background in a pedagogical presentation accessible to students in physics and engineering. From both the theoretical and experimental points of view, four different schemes are discussed in detail; the schemes differ in the way the velocity of the moving pattern of the scattered laser light is measured.

  8. Ultrasonic technique for monitoring of liquid density variations.

    Science.gov (United States)

    Kazys, R; Rekuviene, R; Sliteris, R; Mazeika, L; Zukauskas, E

    2015-01-01

    A novel ultrasonic measurement technique for density measurements of different liquids in extreme conditions has been developed. The proposed density measurement method is based on transformation of the acoustic impedance of the measured liquid. The higher accuracy of measurements is achieved by means of the λ/4 acoustic matching layer between the load and the ultrasonic waveguide transducer. Introduction of the matching layer enhances sensitivity of the measurement system. Sometimes, the density measurements must be performed in very complex conditions: high temperature (up to 200 °C), pressure (up to 10 MPa), and high chemical activity of the medium under measurement. In this case, the special geometry metal waveguides are proposed to use in order to protect the piezoelectric transducer surface from influence of a high temperature. The experimental set-up of technique was calibrated using the reference liquids with different densities: ethyl ether, ethyl alcohol, distilled water, and different concentration (20%, 40%, and 60%) sugar-water solutions. The uncertainty of measurements is less than 1%. The proposed measurement method was verified in real conditions by monitoring the density of a melted polypropylene during manufacturing process.

  9. A density-division embedding potential inversion technique

    Science.gov (United States)

    Roncero, O.; Zanchet, A.; Villarreal, P.; Aguado, A.

    2009-12-01

    A new method is proposed to partition the density of a system in two portions. The density on each subsystem is the solution of a Fock equation modified by the addition of an embedding potential. This embedding potential is obtained iteratively by minimizing the difference between the electronic densities of the total system and the sum of the subsystems. Thus, the electronic density partition and the embedding potential are obtained at the same time within the procedure, guarantying the v-representability of the densities partitioned. This fact is a considerable improvement of a recently proposed embedding potential inversion technique, [O. Roncero, M. P. de Lara-Castells, P. Villarreal, F. Flores, J. Ortega, M. Paniagua, and A. Aguado, J. Chem. Phys. 129, 184104 (2008)], in which the embedding potential is obtained once the electronic density is previously partitioned. The method is first applied to a linear H10 chain to illustrate how it works. The orbitals obtained are localized on each subsystem, and can be used to include local electronic correlation with currently available ab initio programs. Finally, the method is applied to include the electronic correlation needed to describe the van der Waals interaction between H10 chains and H2 molecules, of ≈12 meV, giving very accurate results.

  10. Synchrotron X-ray measurement techniques for thermal barrier coated cylindrical samples under thermal gradients

    Energy Technology Data Exchange (ETDEWEB)

    Siddiqui, Sanna F.; Knipe, Kevin; Manero, Albert; Raghavan, Seetha [Department of Mechanical and Aerospace Engineering, University of Central Florida, Orlando, Florida 32816 (United States); Meid, Carla; Wischek, Janine; Bartsch, Marion [German Aerospace Center (DLR), Institute of Materials Research, 51147 Cologne (Germany); Okasinski, John; Almer, Jonathan [X-Ray Science Division, Advanced Photon Source, Argonne National Laboratory, Argonne, Illinois 60439 (United States); Karlsson, Anette M. [Cleveland State University, 2121 Euclid Avenue, Cleveland, Ohio 44115 (United States)

    2013-08-15

    Measurement techniques to obtain accurate in situ synchrotron strain measurements of thermal barrier coating systems (TBCs) applied to hollow cylindrical specimens are presented in this work. The Electron Beam Physical Vapor Deposition coated specimens with internal cooling were designed to achieve realistic temperature gradients over the TBC coated material such as that occurring in the turbine blades of aeroengines. Effects of the circular cross section on the x-ray diffraction (XRD) measurements in the various layers, including the thermally grown oxide, are investigated using high-energy synchrotron x-rays. Multiple approaches for beam penetration including collection, tangential, and normal to the layers, along with variations in collection parameters are compared for their ability to attain high-resolution XRD data from the internal layers. This study displays the ability to monitor in situ, the response of the internal layers within the TBC, while implementing a thermal gradient across the thickness of the coated sample. The thermal setup maintained coating surface temperatures in the range of operating conditions, while monitoring the substrate cooling, for a controlled thermal gradient. Through variation in measurement location and beam parameters, sufficient intensities are obtained from the internal layers which can be used for depth resolved strain measurements. Results are used to establish the various techniques for obtaining XRD measurements through multi-layered coating systems and their outcomes will pave the way towards goals in achieving realistic in situ testing of these coatings.

  11. Synchrotron X-ray measurement techniques for thermal barrier coated cylindrical samples under thermal gradients.

    Science.gov (United States)

    Siddiqui, Sanna F; Knipe, Kevin; Manero, Albert; Meid, Carla; Wischek, Janine; Okasinski, John; Almer, Jonathan; Karlsson, Anette M; Bartsch, Marion; Raghavan, Seetha

    2013-08-01

    Measurement techniques to obtain accurate in situ synchrotron strain measurements of thermal barrier coating systems (TBCs) applied to hollow cylindrical specimens are presented in this work. The Electron Beam Physical Vapor Deposition coated specimens with internal cooling were designed to achieve realistic temperature gradients over the TBC coated material such as that occurring in the turbine blades of aeroengines. Effects of the circular cross section on the x-ray diffraction (XRD) measurements in the various layers, including the thermally grown oxide, are investigated using high-energy synchrotron x-rays. Multiple approaches for beam penetration including collection, tangential, and normal to the layers, along with variations in collection parameters are compared for their ability to attain high-resolution XRD data from the internal layers. This study displays the ability to monitor in situ, the response of the internal layers within the TBC, while implementing a thermal gradient across the thickness of the coated sample. The thermal setup maintained coating surface temperatures in the range of operating conditions, while monitoring the substrate cooling, for a controlled thermal gradient. Through variation in measurement location and beam parameters, sufficient intensities are obtained from the internal layers which can be used for depth resolved strain measurements. Results are used to establish the various techniques for obtaining XRD measurements through multi-layered coating systems and their outcomes will pave the way towards goals in achieving realistic in situ testing of these coatings.

  12. Reflectometry techniques for density profile measurements on fusion plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Laviron, C. [Association Euratom-CEA, Centre d`Etudes de Cadarache, 13 - Saint-Paul-lez-Durance (France). Dept. de Recherches sur la Fusion Controlee; Donne, A.J.H. [Associatie Euratom-FOM, Nieuwegein (Netherlands). FOM-Instituut voor Plasmafysica; Manso, M.E. [Instituto Superior Tecnico, Lisbon (Portugal). Lab. de Quimica Organica; Sanchez, J. [EURATOM-CIEMAT for Fusion Association, Madrid (Spain)

    1996-03-01

    Reflectometry applied to the measurement of density profiles on fusion plasmas has been subject to many recent developments. After a brief reminder of the principles of reflectometry, the theoretical accuracy of reflectometry measurements is discussed. The main difficulties limiting the performance, namely the plasma fluctuations and the quality of the transmission lines, are analysed. The different techniques used for reflectometry are then presented. The present status and achievements of actual implementations of these techniques are shown, with an analysis of their respective limitations and merits, as well as foreseen developments. (author). 70 refs.

  13. Radial gradients of phase space density in the inner electron radiation

    Science.gov (United States)

    Kim, Kyung-Chan; Shprits, Yuri

    2012-12-01

    While the outer radiation belt (3.5 inner radiation belt (1.2 inner electron belt in recent years. It has been generally accepted that the equilibrium structure of radiation belt electrons is explained by the slow inward radial diffusion from a source in the outer belt and losses by Coulomb collision and wave-particle interaction. In this study, we examine this well accepted theory using the radial profiles of the phase space density (PSD), inferred from in situ measurements made by three different satellites: S3-3, CRRES, and POLAR. Our results show that electron PSD in the inner electron belt has a clear prominent local peak and negative radial gradient in the outer portion of the inner zone, i.e., decreasing PSD with increasingL-value. A likely explanation for the peaks in PSD is acceleration due to energy diffusion produced by lightning-generated and anthropogenic whistlers. These results indicate that either additional local acceleration mechanism is responsible for the formation of the inner electron belt or inner electron belt is formed by sporadic injections of electrons into the inner zone. The currently well accepted model of slow diffusion and losses will be further examined by the upcoming Radiation Belt Storm Probes (RBSP) mission.

  14. Ultrasonic Technique for Density Measurement of Liquids in Extreme Conditions

    Directory of Open Access Journals (Sweden)

    Rymantas Kazys

    2015-08-01

    Full Text Available An ultrasonic technique, invariant to temperature changes, for a density measurement of different liquids under in situ extreme conditions is presented. The influence of geometry and material parameters of the measurement system (transducer, waveguide, matching layer on measurement accuracy and reliability is analyzed theoretically along with experimental results. The proposed method is based on measurement of the amplitude of the ultrasonic wave, reflected from the interface of the solid/liquid medium under investigation. In order to enhance sensitivity, the use of a quarter wavelength acoustic matching layer is proposed. Therefore, the sensitivity of the measurement system increases significantly. Density measurements quite often must be performed in extreme conditions at high temperature (up to 220 °C and high pressure. In this case, metal waveguides between piezoelectric transducer and the measured liquid are used in order to protect the conventional transducer from the influence of high temperature and to avoid depolarization. The presented ultrasonic density measurement technique is suitable for density measurement in different materials, including liquids and polymer melts in extreme conditions. A new calibration algorithm was proposed. The metrological evaluation of the measurement method was performed. The expanded measurement uncertainty Uρ = 7.4 × 10−3 g/cm3 (1%.

  15. A comparison of density functional theory and coupled cluster methods for the calculation of electric dipole polarizability gradients of methane

    DEFF Research Database (Denmark)

    Paidarová, Ivana; Sauer, Stephan P. A.

    2012-01-01

    We have compared the performance of density functional theory (DFT) using five different exchange-correlation functionals with four coupled cluster theory based wave function methods in the calculation of geometrical derivatives of the polarizability tensor of methane. The polarizability gradient...

  16. Collagen density gradient on three-dimensional printed poly(ε-caprolactone) scaffolds for interface tissue engineering.

    Science.gov (United States)

    D'Amora, Ugo; D'Este, Matteo; Eglin, David; Safari, Fatemeh; Sprecher, Christoph M; Gloria, Antonio; De Santis, Roberto; Alini, Mauro; Ambrosio, Luigi

    2017-05-09

    The ability to engineer scaffolds that resemble the transition between tissues would be beneficial to improve repair of complex organs, but has yet to be achieved. In order to mimic tissue organization, such constructs should present continuous gradients of geometry, stiffness and biochemical composition. Although the introduction of rapid prototyping or additive manufacturing techniques allows deposition of heterogeneous layers and shape control, the creation of surface chemical gradients has not been explored on three-dimensional (3D) scaffolds obtained through fused deposition modelling technique. Thus, the goal of this study was to introduce a gradient functionalization method in which a poly(ε-caprolactone) surface was first aminolysed and subsequently covered with collagen via carbodiimide reaction. The 2D constructs were characterized for their amine and collagen contents, wettability, surface topography and biofunctionality. Finally, chemical gradients were created in 3D printed scaffolds with controlled geometry and porosity. The combination of additive manufacturing and surface modification is a viable tool for the fabrication of 3D constructs with controlled structural and chemical gradients. These constructs can be employed for mimicking continuous tissue gradients for interface tissue engineering. Copyright © 2017 John Wiley & Sons, Ltd.

  17. Application of density gradient for the isolation of the fecal microbial stool component and the potential use thereof.

    Science.gov (United States)

    Hevia, Arancha; Delgado, Susana; Margolles, Abelardo; Sánchez, Borja

    2015-11-19

    The idea of considering the gut microbiota as a virtual human organ has led to the concept of fecal microbiota transplantation (FMT), which has recently been extremely successful in the treatment of cases of recurrent Clostridium difficile infection. Administration of safe, viable, and representative fecal microbiota is crucial for FMT. To our knowledge, suitable techniques and systematic conditions for separating the fecal microbiota from stool samples have not been thoroughly investigated. In this work we show the potential to separate stool microorganisms from the rest of fecal material using a procedure with a Nycodenz® density gradient, yielding 10(10) viable bacteria per two grams of feces. This procedure did not affect the original microbiota composition in terms of viability, distribution and proportions, as assessed by a phylogenetic metagenomic approach. Obtaining the fecal microbiota by concentration and separation of the microorganisms from the rest of the stool components would allow the standardization of its recovery and its long-term preservation. FMT or similar microbiota restoration therapies could be used for the treatment of several disorders, or even for aesthetic purposes, so the method described in our work may contribute to the setting of the basis for the development of safe and standardized products.

  18. Gradient measurement technique to identify phase transitions in nano-dispersed liquid crystalline compounds

    Science.gov (United States)

    Pardhasaradhi, P.; Madhav, B. T. P.; Venugopala Rao, M.; Manepalli, R. K. N. R.; Pisipati, V. G. K. M.

    2016-09-01

    Characterization and phase transitions in pure and 0.5% BaTiO3 nano-dispersed liquid crystalline (LC) N-(p-n-heptyloxybenzylidene)-p-n-nonyloxy aniline, 7O.O9, com-pounds are carried out using a polarizing microscope attached with hot stage and camera. We observed that when any of these images are distorted, different local structures suffer from various degradations in a gradient magnitude. So, we examined the pixel-wise gradient magnitude similarity between the reference and distorted images combined with a novel pooling strategy - the standard deviation of the GMS map - to determine the overall phase transition variations. In this regard, MATLAB software is used for gradient measurement technique to identify the phase transitions and transition temperature of the pure and nano-dispersed LC compounds. The image analysis of this method proposed is in good agreement with the standard methods like polarizing microscope (POM) and differential scanning calorimeter (DSC). 0.5% BaTiO3 nano-dispersed 7O.O9 compound induces cholesteric phase quenching the nematic phase, which the pure compound exhibits.

  19. Soot particle density determination from a laser extinction multipass technique

    Science.gov (United States)

    Glaros, Gregory E.

    1994-09-01

    Methods of measuring soot particle densities have been of interest for several decades. Plume signature determination of both rocket and air-breathing engines is of concern when applied to pollution and theater missile ballistic defense strategies. Application of non-intrusive traditional techniques employing Bouguer's law relied on Sauter mean diameter statistical deviation and the probability density function in order to compensate for the ambiguities present in the extension of classical Mie theory. Our investigation developed an apparatus which will determine soot particle densities by measuring extinction from absorption of light energy transmitted through an exhaust plume. The method used was a two-pass technique using an optical phase conjugator (OPC) which returned the non-absorbed portion of light energy. When the apparatus was used with a retroreflector, it produced accurate results but did not compensate for thermal blooming or beam steering. Characteristics of a photorefractive crystal used in the QPC process allowed for the return of an incident beam corrected for aberrations. Although the OPC returned the phase conjugate of the incident beam its size precluded the return of all of the transmitted data because data was lost on the blossomed beam.

  20. A model for thin layer formation by delayed particle settling at sharp density gradients

    Science.gov (United States)

    Prairie, Jennifer C.; White, Brian L.

    2017-02-01

    Thin layers - regions where plankton or particles accumulate vertically on scales of a few meters or less - are common in coastal waters, and have important implications for both trophic dynamics and carbon cycling. These features can form by a variety of biological and physical mechanisms, including localized growth, shear-thinning, and directed swimming. An additional mechanism may result in the formation of thin layers of marine aggregates, which have been shown to decrease their settling velocity when passing through sharp density gradients, a behavior termed delayed settling. Here, we apply a simple vertical advection-diffusion model to predict the properties of aggregate thin layers formed by this process. We assume a constant vertical flux of particles from the surface, which is parameterized by observations from laboratory experiments with marine aggregates. The formation, maintenance, and shape of the layers are described in relation to non-dimensional numbers that depend on environmental conditions and particle settling properties. In particular, model results demonstrate layer intensity and sharpness both increase with higher Péclet number (Pe), that is, under conditions with weaker mixing relative to layer formation. Similarly, more intense and sharper layers are found when the delayed settling behavior of aggregates is characterized by a lower velocity minimum. The model also predicts layers that are vertically asymmetric and highly "peaky" when compared with a Gaussian distribution, features often seen in thin layers in natural environments. Lastly, by comparing model predictions with observations of thin layers in the field, we are able to gain some insight into the applicability of delayed settling as a thin layer formation mechanism in different environmental conditions.

  1. New signal processing technique for density profile reconstruction using reflectometry

    Energy Technology Data Exchange (ETDEWEB)

    Clairet, F.; Bottereau, C. [CEA, IRFM, F-13108 Saint-Paul-lez-Durance (France); Ricaud, B. [CEA, IRFM, F-13108 Saint-Paul-lez-Durance (France); CPT UMR 6207, Campus de Luminy, case 907, F-13288 Marseille (France); Briolle, F. [CPT UMR 6207, Campus de Luminy, case 907, F-13288 Marseille (France); CReA, BA 701, F-13306 Salon de Provence (France); Heuraux, S. [IJL-P2M, UMR-CNRS 7198, Universite Henri Poincare, F-54506 Vandoeuvre (France)

    2011-08-15

    Reflectometry profile measurement requires an accurate determination of the plasma reflected signal. Along with a good resolution and a high signal to noise ratio of the phase measurement, adequate data analysis is required. A new data processing based on time-frequency tomographic representation is used. It provides a clearer separation between multiple components and improves isolation of the relevant signals. In this paper, this data processing technique is applied to two sets of signals coming from two different reflectometer devices used on the Tore Supra tokamak. For the standard density profile reflectometry, it improves the initialization process and its reliability, providing a more accurate profile determination in the far scrape-off layer with density measurements as low as 10{sup 16} m{sup -1}. For a second reflectometer, which provides measurements in front of a lower hybrid launcher, this method improves the separation of the relevant plasma signal from multi-reflection processes due to the proximity of the plasma.

  2. Visualization techniques for spatial probability density function data

    Directory of Open Access Journals (Sweden)

    Udeepta D Bordoloi

    2006-01-01

    Full Text Available Novel visualization methods are presented for spatial probability density function data. These are spatial datasets, where each pixel is a random variable, and has multiple samples which are the results of experiments on that random variable. We use clustering as a means to reduce the information contained in these datasets; and present two different ways of interpreting and clustering the data. The clustering methods are used on two datasets, and the results are discussed with the help of visualization techniques designed for the spatial probability data.

  3. Cost-Optimal Design of a 3-Phase Core Type Transformer by Gradient Search Technique

    Science.gov (United States)

    Basak, R.; Das, A.; Sensarma, A. K.; Sanyal, A. N.

    2014-04-01

    3-phase core type transformers are extensively used as power and distribution transformers in power system and their cost is a sizable proportion of the total system cost. Therefore they should be designed cost-optimally. The design methodology for reaching cost-optimality has been discussed in details by authors like Ramamoorty. It has also been discussed in brief in some of the text-books of electrical design. The paper gives a method for optimizing design, in presence of constraints specified by the customer and the regulatory authorities, through gradient search technique. The starting point has been chosen within the allowable parameter space the steepest decent path has been followed for convergence. The step length has been judiciously chosen and the program has been maneuvered to avoid local minimal points. The method appears to be best as its convergence is quickest amongst different optimizing techniques.

  4. Analytical gradients of the state-average complete active space self-consistent field method with density fitting

    Energy Technology Data Exchange (ETDEWEB)

    Delcey, Mickaël G. [Department of Chemistry – Ångström, The Theoretical Chemistry Programme, Uppsala University, P.O. Box 518, 751 20 Uppsala (Sweden); Pedersen, Thomas Bondo [Centre for Theoretical and Computational Chemistry, Department of Chemistry, University of Oslo, P.O. Box 1033 Blindern, 0315 Oslo (Norway); Aquilante, Francesco [Department of Chemistry – Ångström, The Theoretical Chemistry Programme, Uppsala University, P.O. Box 518, 751 20 Uppsala (Sweden); Dipartimento di chimica “G. Ciamician,” Università di Bologna, V. F. Selmi 2, 40126 Bologna (Italy); Lindh, Roland, E-mail: roland.lindh@kemi.uu.se [Department of Chemistry – Ångström, The Theoretical Chemistry Programme, Uppsala University, P.O. Box 518, 751 20 Uppsala (Sweden); Uppsala Center for Computational Chemistry - UC_3, Uppsala University, P.O. Box 518, 751 20 Uppsala (Sweden)

    2015-07-28

    An efficient implementation of the state-averaged complete active space self-consistent field (SA-CASSCF) gradients employing density fitting (DF) is presented. The DF allows a reduction both in scaling and prefactors of the different steps involved. The performance of the algorithm is demonstrated on a set of molecules ranging up to an iron-Heme b complex which with its 79 atoms and 811 basis functions is to our knowledge the largest SA-CASSCF gradient computed. For smaller systems where the conventional code could still be used as a reference, both the linear response calculation and the gradient formation showed a clear timing reduction and the overall cost of a geometry optimization is typically reduced by more than one order of magnitude while the accuracy loss is negligible.

  5. Isolation of Plasmodium berghei ookinetes in culture using Nycodenz density gradient columns and magnetic isolation

    Directory of Open Access Journals (Sweden)

    Williams Jackie

    2003-11-01

    Full Text Available Abstract Background Large scale in vitro production of the mosquito stages of malaria parasites remains elusive, with only limited success for complete sporogonic development and only one report of development through to infective sporozoites. The initial step in this process is the production, in vitro, of ookinetes from gametocytaemic blood. Methods for isolation of these ookinetes from blood cells have been described; however, in addition to yield often being low, processing time and potential for contamination by erythrocytes remain high. Methods This study compares two procedures for retaining mature ookinetes from blood stage cultures, whilst removing red blood cells and other contaminants prior to further culture of the parasite. The well established method of isolation on Nycodenz cushions is compared with a novel method utilizing the innate magnetic properties of the haem pigment crystals found in the cytoplasm of ookinetes. Results Yield and viability of ookinetes were similar with both isolation methods. However, in our hands magnetic isolation produced a cleaner ookinete preparation much more quickly. Moreover, decreasing the flow rate through the magnetic column could further enhance the yield. Conclusion We recommend the enrichment of an ookinete preparation prior to further culture being performed using the magnetic properties of Plasmodium berghei ookinetes as an alternative to their density. The former technique is faster, removes more erythrocytes, but day-to-day costs are greater.

  6. Enhanced Stereo Matching Technique using Image Gradient for Improved Search Time

    Directory of Open Access Journals (Sweden)

    Pratibha Vellanki

    2011-05-01

    Full Text Available Stereo matching algorithms developed from local area based correspondence matching involve extensive search. This paper presents a stereo matching technique for computation of a dense disparity map by trimming down the search time for the local area based correspondence matching. We use constraints such as epipolar line, limiting the disparity, uniqueness and continuity to obtain an initial dense disparity map. We attempt to improvise this map by using color information for matching. A new approach has been discussed which is based on the extension of the continuity constraint for reducing the search time. We use correspondence between rows and gradient of image to compute the disparity. Thus we achieve a good trade off between accuracy and search time.

  7. Development of gradient-corrected exchange-correlation functionals in the density functional theory; Developpement de fonctionnelles corrigees du gradient en theorie de la fonctionnelle de la densite

    Energy Technology Data Exchange (ETDEWEB)

    Lembarki, A.

    1994-12-01

    In this work, we have developed some gradient-corrected exchange-correlation functionals. This study is in keeping with the density functional theory (DFT) formalism. In the first part of this memory, a description of Hartree-Fock (HF), post-HF and density functional theories is given. The second part is devoted the study the different approximations of DFT exchange-correlation functionals which have been proposed in the last years. In particular, we have underlined the approximations used for the construction of these functionals. The third part of this memory consists in the development of new gradient-corrected functionals. In this study, we have established a new relation between exchange energy, correlation energy and kinetic energy. We have deduced two new possible forms of exchange or correlation functionals, respectively. In the fourth part, we have studied the exchange potential, for which the actual formulation does not satisfy some theoretical conditions, such as the asymptotic behavior -1/r. Our contribution lies in the development of an exchange potential with a correct asymptotic -1/r behavior for large values of r. In this chapter, we have proposed a model which permits the obtention of the exchange energy from the exchange potential, using the virial theorem. The fifth part of this memory is devoted the application of these different functionals to simple systems (H{sub 2}O, CO, N{sub 2}O, H{sub 3}{sup +} and H{sub 5}{sup +}) in order to characterize the performance of DFT calculations in regards to those obtained with post-HF methods. (author). 215 refs., 8 figs., 28 tabs.

  8. Different elution modes and field programming in gravitational field-flow fractionation: field programming using density and viscosity gradients.

    Science.gov (United States)

    Plocková, Jana; Chmelík, Josef

    2006-06-23

    In previous papers, several approaches to programming of the resulting force field in GFFF were described and investigated. The experiments were dealing with flow-velocity and channel thickness, i.e. factors influencing hydrodynamic lift forces (HLF). The potential of density and viscosity of carrier liquid for field programming was predicted and demonstrated by preliminary experiments. This work is devoted to experimental verification of the influence of carrier liquid density and viscosity. Several carrier liquid density and simultaneously viscosity gradients using water-methanol mixtures are in this work implemented in the separation of a model silica mixture. Working with the water-methanol gradients, one is not able to separate the influence of density from the contribution of viscosity. However, we found experimental conditions to show the isolated effect of carrier liquid density (two water-methanol mixtures of equal viscosity differing in their densities). In order to demonstrate the isolated effect of viscosity, we implemented in this work a new system of (hydroxypropyl)methyl cellulose (HPMC) carrier liquids. Three different HPMC compositions enabled to vary the viscosity more than two times at almost constant density. With increasing carrier liquid viscosity, the focusing and elevating trend was clearly pronounced for 5 and 10 microm silica particles. By the isolated effect of increased viscosity, the centre of the 10 microm particle zone was elevated to the streamline at 16% of the channel height. These experiments have shown that the influence of carrier liquid viscosity on HLF should be taken into account even at higher levels above the channel bottom, i.e. beyond the near-wall region. Further, it is shown that higher value of carrier liquid viscosity improves the separation of the model mixture in terms of time and resolution.

  9. Analytical nuclear excited-state gradients for the Tamm-Dancoff approximation using uncoupled frozen-density embedding.

    Science.gov (United States)

    Heuser, Johannes; Höfener, Sebastian

    2017-10-15

    We report the derivation and implementation of analytical nuclear gradients for excited states using time-dependent density functional theory using the Tamm-Dancoff approximation combined with uncoupled frozen-density embedding using density fitting. Explicit equations are presented and discussed. The implementation is able to treat singlet as well as triplet states and functionals using the local density approximation, the generalized gradient approximation, combinations with Hartree-Fock exchange (hybrids), and range-separated functionals such as CAM-B3LYP. The new method is benchmarked against supermolecule calculations in two case studies: The solvatochromic shift of the (vertical) fluorescence energy of 4-aminophthalimide on solvation, and the first local excitation of the benzonitrile dimer. Whereas for the 4-aminophthalimide-water complex deviations of about 0.2 eV are obtained to supermolecular calculations, for the benzonitrile dimer the maximum error for adiabatic excitation energies is below 0.01 eV due to a weak coupling of the subsystems. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  10. Structured DC Electric Fields With and Without Associated Plasma Density Gradients Observed with the C/NOFS Satellite

    Science.gov (United States)

    Pfaff, R.; Rowland, D.; Klenzing, J.; Freudenreich, H.; Bromund, K.; Liebrecht, C.; Roddy, P.; Hunton, D.

    2009-01-01

    DC electric field observations and associated plasma drifts gathered with the Vector Electric Field Investigation on the Air Force Communication/Navigation Outage Forecasting System (C/NOFS) satellite typically reveal considerable variation at large scales (approximately 100's of km), in both daytime and nighttime cases, with enhanced structures usually confined to the nightside. Although such electric field structures are typically associated with plasma density depletions and structures, as observed by the Planar Langmuir Probe on C/NOFS, what is surprising is the number of cases in which large amplitude, structured DC electric fields are observed without a significant plasma density counterpart structure, including their appearance at times when the ambient plasma density appears relatively quiescent. We investigate the relationship of such structured DC electric fields and the ambient plasma density in the C/NOFS satellite measurements observed thus far, taking into account both plasma density depletions and enhancements. We investigate the mapping of the electric fields along magnetic field lines from distant altitudes and latitudes to locations where the density structures, which presumably formed the original seat of the electric fields, are no longer discernible in the observations. In some cases, the electric field structures and spectral characteristics appear to mimic those associated with equatorial spread-F processes, providing important clues to their origins. We examine altitude, seasonal, and longitudinal effects in an effort to establish the origin of such structured DC electric fields observed both with, and without, associated plasma density gradients

  11. Correlation between density fluctuations and plasma gradients at the edge of the TORE SUPRA tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Devynck, P.; Garbet, X.; Laviron, C.; Payan, J.; Haas, J. de; Clairet, F.; Talvard, M. [Association Euratom-CEA, Centre d`Etudes de Cadarache, 13 - Saint-Paul-lez-Durance (France). Dept. de Recherches sur la Fusion Controlee; Gervais, F.; Gresillon, D.; Hennequin, P.; Quemeneur, A.; Truc, A. [Ecole Polytechnique, 91 - Palaiseau (France). Lab. de Physique des Milieux Ionises

    1993-12-31

    The ALTAIR diagnostic uses scattering on the plasma electrons with the help of an IR laser beam to obtain information on the density fluctuations. This diagnostic can simultaneously record fluctuations from two independent wave numbers. Two experiments are carried out: study of the density fluctuations during strong plasma heating that combined both low hybrid waves and ion cyclotron heating; density fluctuations associated to the tokamak gas-feed modulation. 6 figs., 5 refs.

  12. [The study of the growth of tylosin producer using differential centrifugation of mycelium in a sucrose density gradient].

    Science.gov (United States)

    Salamakha, O V; Rogatykh, N P; Savochkina, I V; Tikhomirova, L A; Bazarenko, I L

    1989-01-01

    The mycelium of Streptomyces fradiae was fractionated by differential centrifugation in a sucrose density gradient (SDG) using various samples of the inoculation material and aliquots of the cultural broth taken in the course of tylosin production. The mode of mycelium distribution in SDG made it possible to select the most active inoculation material. The mycelium was redistributed from sucrose layers with a high density to those with a lower density in the course of fermentation. The fractions differed in the antibiotic activity but none of them had an activity higher than in the control centrifuged in 30% sucrose and washed off just like the fractions. Therefore, mycelium fractionation in SDG would not elevate its antibiotic activity. The paper presents the cytological characteristics of different fractions changing in the course of fermentation.

  13. Laplacian-dependent models of the kinetic energy density: Applications in subsystem density functional theory with meta-generalized gradient approximation functionals.

    Science.gov (United States)

    Śmiga, Szymon; Fabiano, Eduardo; Constantin, Lucian A; Della Sala, Fabio

    2017-02-14

    The development of semilocal models for the kinetic energy density (KED) is an important topic in density functional theory (DFT). This is especially true for subsystem DFT, where these models are necessary to construct the required non-additive embedding contributions. In particular, these models can also be efficiently employed to replace the exact KED in meta-Generalized Gradient Approximation (meta-GGA) exchange-correlation functionals allowing to extend the subsystem DFT applicability to the meta-GGA level of theory. Here, we present a two-dimensional scan of semilocal KED models as linear functionals of the reduced gradient and of the reduced Laplacian, for atoms and weakly bound molecular systems. We find that several models can perform well but in any case the Laplacian contribution is extremely important to model the local features of the KED. Indeed a simple model constructed as the sum of Thomas-Fermi KED and 1/6 of the Laplacian of the density yields the best accuracy for atoms and weakly bound molecular systems. These KED models are tested within subsystem DFT with various meta-GGA exchange-correlation functionals for non-bonded systems, showing a good accuracy of the method.

  14. Fractionation of Saprolegnia diclina (Oomycetes) satelite DNAs by AgNO3/Cs2SO4 density gradient centrifugation.

    Science.gov (United States)

    Neish, G A; Green, B R

    1977-12-14

    Saprolegnia diclina DNA has been fractionated using preparative AgNO3/Cs2SO4 and CsCl density gradients. In addition to the previously identified major satellite DNA, there are two minor DNA components banding at 1.682 and 1.701 g - cm(-3) in CsCl. Purified major satellite DNA bands at 1.707 g - cm(-3) giving a base composition of 48% G + C in good agreement with 47% G + C calculated from its Tm value. The nuclear DNA base composition is 58% G + C by both methods. The base composition of the major satellite DNA suggests that it may represent ribosomal DNA cistrons.

  15. Mapping axonal density and average diameter using non-monotonic time-dependent gradient-echo MRI

    DEFF Research Database (Denmark)

    Nunes, Daniel; Cruz, Tomás L; Jespersen, Sune N

    2017-01-01

    Abstract White Matter (WM) microstructures, such as axonal density and average diameter, are crucial to the normal function of the Central Nervous System (CNS) as they are closely related with axonal conduction velocities. Conversely, disruptions of these microstructural features may result....... While clearly further modelling and theoretical developments are necessary, we conclude that salient WM microstructural features can be extracted from these simple, SNR-efficient multi-gradient echo MRI, and that this paves the way towards easier estimation of WM microstructure in vivo....

  16. Automated liver sampling using a gradient dual-echo Dixon-based technique.

    Science.gov (United States)

    Bashir, Mustafa R; Dale, Brian M; Merkle, Elmar M; Boll, Daniel T

    2012-05-01

    Magnetic resonance spectroscopy of the liver requires input from a physicist or physician at the time of acquisition to insure proper voxel selection, while in multiecho chemical shift imaging, numerous regions of interest must be manually selected in order to ensure analysis of a representative portion of the liver parenchyma. A fully automated technique could improve workflow by selecting representative portions of the liver prior to human analysis. Complete volumes from three-dimensional gradient dual-echo acquisitions with two-point Dixon reconstruction acquired at 1.5 and 3 T were analyzed in 100 subjects, using an automated liver sampling algorithm, based on ratio pairs calculated from signal intensity image data as fat-only/water-only and log(in-phase/opposed-phase) on a voxel-by-voxel basis. Using different gridding variations of the algorithm, the average correct liver volume samples ranged from 527 to 733 mL. The average percentage of sample located within the liver ranged from 95.4 to 97.1%, whereas the average incorrect volume selected was 16.5-35.4 mL (2.9-4.6%). Average run time was 19.7-79.0 s. The algorithm consistently selected large samples of the hepatic parenchyma with small amounts of erroneous extrahepatic sampling, and run times were feasible for execution on an MRI system console during exam acquisition. Copyright © 2011 Wiley Periodicals, Inc.

  17. Thermodynamic, energy efficiency, and power density analysis of reverse electrodialysis power generation with natural salinity gradients.

    Science.gov (United States)

    Yip, Ngai Yin; Vermaas, David A; Nijmeijer, Kitty; Elimelech, Menachem

    2014-05-06

    Reverse electrodialysis (RED) can harness the Gibbs free energy of mixing when fresh river water flows into the sea for sustainable power generation. In this study, we carry out a thermodynamic and energy efficiency analysis of RED power generation, and assess the membrane power density. First, we present a reversible thermodynamic model for RED and verify that the theoretical maximum extractable work in a reversible RED process is identical to the Gibbs free energy of mixing. Work extraction in an irreversible process with maximized power density using a constant-resistance load is then examined to assess the energy conversion efficiency and power density. With equal volumes of seawater and river water, energy conversion efficiency of ∼ 33-44% can be obtained in RED, while the rest is lost through dissipation in the internal resistance of the ion-exchange membrane stack. We show that imperfections in the selectivity of typical ion exchange membranes (namely, co-ion transport, osmosis, and electro-osmosis) can detrimentally lower efficiency by up to 26%, with co-ion leakage being the dominant effect. Further inspection of the power density profile during RED revealed inherent ineffectiveness toward the end of the process. By judicious early discontinuation of the controlled mixing process, the overall power density performance can be considerably enhanced by up to 7-fold, without significant compromise to the energy efficiency. Additionally, membrane resistance was found to be an important factor in determining the power densities attainable. Lastly, the performance of an RED stack was examined for different membrane conductivities and intermembrane distances simulating high performance membranes and stack design. By thoughtful selection of the operating parameters, an efficiency of ∼ 37% and an overall gross power density of 3.5 W/m(2) represent the maximum performance that can potentially be achieved in a seawater-river water RED system with low

  18. Combination of direct swim-up technique and discontinuous Percoll gradient centrifugation for sperm preparation of oligoasthenozoospermic samples.

    Science.gov (United States)

    Chen, S U; Ho, H N; Chen, H F; Chao, K H; Wu, M Y; Chen, C D; Huang, S C; Lee, T Y; Yang, Y S

    1996-01-01

    Sperm recovery for assisted reproduction in oligoasthenozoospermic patients is not satisfying either by the swim-up technique or by Percoll gradient centrifugation, and no single technique is constantly preferred. The design of this study was to evaluate the effects of combining the two methods on improving the efficacy of sperm preparation in these poor samples. For each semen sample, 1 mL was treated with a combination method, which used direct swim-up technique to recover motile sperm swimming to the supernatant, and then the residual semen was subjected to two-layer discontinuous Percoll gradient procedure for further recovery of motile sperm. Another 1 mL was prepared with two-layer discontinuous Percoll gradient centrifugation alone for comparison. Parameters measured included sperm concentration, number of progressively motile sperm, percentage of progressive motility, percentage of motile sperm recovery, amount of debris, percentage of normal forms according to Kruger's strict criteria, and motion characteristics of sperm using computer-aided motility analysis. The results of 30 oligoasthenozoospermic samples demonstrated that the combination method achieved a significantly greater recovery of motile sperm than the two-layer discontinuous Percoll gradient centrifugation did (43.2 +/- 19.7% vs. 32.2 +/- 14.3%, p method than in those of Percoll gradient centrifugation alone, but the difference was not significant (63.7 +/- 21.8% vs. 58.7 +/- 20.1%). The debris of semen was removed equally well by both methods. The percentage of normal forms as well as motion characteristics, including curvilinear velocity, straight-line velocity, mean amplitude of lateral head displacement, and linearity, were similar in the samples treated by these two procedures. The combination of the direct swim-up technique and discontinuous Percoll gradient centrifugation combines the advantages of each procedure and results in greater recovery of motile sperm in oligoasthenozoospermic

  19. Alternative sample-introduction technique to avoid breakthrough in gradient-elution liquid chromatography of polymers

    NARCIS (Netherlands)

    Reingruber, E.; Bedani, F.; Buchberger, W.; Schoenmakers, P.

    2010-01-01

    Gradient-elution liquid chromatography (GELC) is a powerful tool for the characterization of synthetic polymers. However, gradient-elution chromatograms often suffer from breakthrough phenomena. Breakthrough can be averted by using a sample solvent as weak as the mobile phase. However, this approach

  20. Up-Hill Diffusion Creating Density Gradient - What is the Proper Entropy?

    CERN Document Server

    Sato, Naoki

    2016-01-01

    It is always some constraint that yields any nontrivial structure from statistical averages. As epitomized by the Boltzmann distribution, the energy conservation is often the principal constraint acting on mechanical systems. Here, we investigate a different type: the topological constraint imposed on `space'. Such constraint emerges from the null space of the Poisson operator linking energy gradient to phase space velocity, and appears as an adiabatic invariant altering the preserved phase space volume at the core of statistical mechanics. The correct measure of entropy, built on the distorted invariant measure, behaves consistently with the second law of thermodynamics. The opposite behavior (decreasing entropy and negative entropy production) arises in arbitrary coordinates. An ensamble of rotating rigid bodies is worked out. The theory is then applied to up-hill diffusion in a magnetosphere.

  1. GLOBAL CONVERGENCE RESULTS OF A THREE TERM MEMORY GRADIENT METHOD WITH A NON-MONOTONE LINE SEARCH TECHNIQUE

    Institute of Scientific and Technical Information of China (English)

    Sun Qingying

    2005-01-01

    In this paper, a new class of three term memory gradient method with nonmonotone line search technique for unconstrained optimization is presented. Global convergence properties of the new methods are discussed. Combining the quasi-Newton method with the new method, the former is modified to have global convergence property. Numerical results show that the new algorithm is efficient.

  2. A process study of the interaction of tidal currents, tidal mixing and density gradients in a region of freshwater influence

    Science.gov (United States)

    Xing, Jiuxing; Chen, Shengli

    2017-08-01

    A three dimensional unstructured grid model of the west coast of Britain is used to study the process of the interaction of tidal currents, tidal mixing and density gradient in the Liverpool Bay region. Calculations with M2 tidal forcing and omitting freshwater discharge show that tidal currents in the region are strong (of order 1 ms- 1), with tidal current ellipses essentially rectilinear in the surface and bottom. In the absence of tidal forcing, the freshwater is confined to a thin surface layer. With the inclusion of tidal mixing the surface layer thickens, and in the shallow water area mixed layer occupies the whole water depth. This has a significant effect of reducing its lateral spread. A detailed study of time series of velocity, salinity and turbulence reveals that at flood tide, more saline water is advected into the coastal region and rapid vertical mixing occurs, whereas at ebb tide, fresher water is advected over more saline water. The induced strong pycnocline uncouples surface and bottom layers leading to more circular tidal ellipses which rotate in opposite directions in the vertical, as found in observations. The three dimensional nature of the model reveals that this process involves both horizontal and vertical density gradients, and shows significant horizontal variability in the Liverpool Bay region.

  3. Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation: implications for assisted conception.

    Science.gov (United States)

    Tomlinson, M J; Moffatt, O; Manicardi, G C; Bizzaro, D; Afnan, M; Sakkas, D

    2001-10-01

    With an increase in the use of assisted reproduction technologies the requirements of the diagnostic semen analysis are constantly changing. Spermatozoa from patients undergoing IVF were analysed by examining the conventional semen parameters and DNA/chromatin integrity, using in-situ nick translation (NT) and the Chromomycin A(3) fluorochrome, which indirectly demonstrates a decreased presence of protamine. Samples were examined before and after preparation using discontinuous density gradient centrifugation. Density gradient centrifugation enriched samples by improving the percentage of morphologically normal forms by 138% and sperm nuclear integrity by 450%. Sperm nuclear integrity as assessed by in-situ nick translation (NT) demonstrated a very clear relationship with sperm concentration, motility and morphology. Morphology correlated with fertilization rates of patients undergoing IVF, while NT values of the spermatozoa post-preparation were significantly lower in pregnant patients. We have demonstrated that along with the classical semen parameters, the assessment of nuclear integrity improves the characterization of the semen sample and may be used as a tool for allocating patients to specific assisted reproduction treatments.

  4. Damping-Growth Transition for Ion-Acoustic Waves in a Density Gradient

    DEFF Research Database (Denmark)

    D'Angelo, N.; Michelsen, Poul; Pécseli, Hans

    1975-01-01

    A damping-growth transition for ion-acoustic waves propagating in a nonuniform plasma (e-folding length for the density ln) is observed at a wavelength λ∼2πln. This result supports calculations performed in connection with the problem of heating of the solar corona by ion-acoustic waves generated...

  5. Numerical Studies of Electron Acceleration Behind Self-Modulating Proton Beam in Plasma with a Density Gradient

    CERN Document Server

    Petrenko, Alexey; Sosedkin, Alexander

    2016-01-01

    Presently available high-energy proton beams in circular accelerators carry enough momentum to accelerate high-intensity electron and positron beams to the TeV energy scale over several hundred meters of the plasma with a density of about 1e15 1/cm^3. However, the plasma wavelength at this density is 100-1000 times shorter than the typical longitudinal size of the high-energy proton beam. Therefore the self-modulation instability (SMI) of a long (~10 cm) proton beam in the plasma should be used to create the train of micro-bunches which would then drive the plasma wake resonantly. Changing the plasma density profile offers a simple way to control the development of the SMI and the acceleration of particles during this process. We present simulations of the possible use of a plasma density gradient as a way to control the acceleration of the electron beam during the development of the SMI of a 400 GeV proton beam in a 10 m long plasma. This work is done in the context of the AWAKE project --- the proof-of-prin...

  6. A Study on Effects on Current Density Distribution, Inductance Gradient, and Contact Force by Variation of Armature and Rail Structure

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Bok Ki [Kwangwoon University, Seoul (Korea)

    2001-02-01

    The distribution of current in the conductors influenced by armature geometry and velocity is an important parameter for determining performance of an electromagnetic launcher(EML). The electric current in the early launching stage tends to flow on the outer surface of the conductors, resulting in very high local electric current density. However, the tendency for current to concentrate on the surface is driven by the velocity skin effect later in launching stage. The high current density produces high local heating and, consequently, increases armature wear which causes several defects on EML system. This paper investigates the effect of rail/armature geometry on current density distribution, launcher inductance gradient (L'), and contact force. Three geometrical parameters are used to characterize the railgun system. These are the ratio of contact length, relative position of contact leading edge to root trailing edge, and the ratio of rail overhang to the rail height. The distribution of current density, L',contact force between various configurations of the armature and the rail are analyzed and compared by using the EMAP3D program. (author). 6 refs., 9 figs., 3 tabs.

  7. Mapping the genome of meta-generalized gradient approximation density functionals: The search for B97M-V

    Energy Technology Data Exchange (ETDEWEB)

    Mardirossian, Narbe [Kenneth S. Pitzer Center for Theoretical Chemistry, Department of Chemistry, University of California, Berkeley, California 94720 (United States); Head-Gordon, Martin, E-mail: mhg@cchem.berkeley.edu [Kenneth S. Pitzer Center for Theoretical Chemistry, Department of Chemistry, University of California, Berkeley, California 94720 (United States); Chemical Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States)

    2015-02-21

    A meta-generalized gradient approximation density functional paired with the VV10 nonlocal correlation functional is presented. The functional form is selected from more than 10{sup 10} choices carved out of a functional space of almost 10{sup 40} possibilities. Raw data come from training a vast number of candidate functional forms on a comprehensive training set of 1095 data points and testing the resulting fits on a comprehensive primary test set of 1153 data points. Functional forms are ranked based on their ability to reproduce the data in both the training and primary test sets with minimum empiricism, and filtered based on a set of physical constraints and an often-overlooked condition of satisfactory numerical precision with medium-sized integration grids. The resulting optimal functional form has 4 linear exchange parameters, 4 linear same-spin correlation parameters, and 4 linear opposite-spin correlation parameters, for a total of 12 fitted parameters. The final density functional, B97M-V, is further assessed on a secondary test set of 212 data points, applied to several large systems including the coronene dimer and water clusters, tested for the accurate prediction of intramolecular and intermolecular geometries, verified to have a readily attainable basis set limit, and checked for grid sensitivity. Compared to existing density functionals, B97M-V is remarkably accurate for non-bonded interactions and very satisfactory for thermochemical quantities such as atomization energies, but inherits the demonstrable limitations of existing local density functionals for barrier heights.

  8. Numerical studies of electron acceleration behind self-modulating proton beam in plasma with a density gradient

    Science.gov (United States)

    Petrenko, A.; Lotov, K.; Sosedkin, A.

    2016-09-01

    Presently available high-energy proton beams in circular accelerators carry enough momentum to accelerate high-intensity electron and positron beams to the TeV energy scale over several hundred meters of the plasma with a density of about 1015cm-3. However, the plasma wavelength at this density is 100-1000 times shorter than the typical longitudinal size of the high-energy proton beam. Therefore the self-modulation instability (SMI) of a long (~10 cm) proton beam in the plasma should be used to create the train of micro-bunches which would then drive the plasma wake resonantly. Changing the plasma density profile offers a simple way to control the development of the SMI and the acceleration of particles during this process. We present simulations of the possible use of a plasma density gradient as a way to control the acceleration of the electron beam during the development of the SMI of a 400 GeV proton beam in a 10 m long plasma. This work is done in the context of the AWAKE project-the proof-of-principle experiment on proton driven plasma wakefield acceleration at CERN.

  9. Particulate emissions from a beef cattle feedlot using the flux-gradient technique.

    Science.gov (United States)

    Bonifacio, Henry F; Maghirang, Ronaldo G; Trabue, Steven L; McConnell, Laura L; Prueger, John H; Razote, Edna B

    2014-07-01

    Data on air emissions from open-lot beef cattle () feedlots are limited. This research was conducted to determine fluxes of particulate matter with an aerodynamic diameter ≤10 μm (PM) from a commercial beef cattle feedlot in Kansas using the flux-gradient technique, a widely used micrometeorological method for air emissions from open sources. Vertical PM concentration profiles and micrometeorological parameters were measured at the feedlot using tapered element oscillating microbalance PM samplers and eddy covariance instrumentations (i.e., sonic anemometer and infrared hygrometer), respectively, from May 2010 through September 2011, representing feedlot conditions with air temperatures ranging from -24 to 39°C. Calculated hourly PM fluxes varied diurnally and seasonally, ranging up to 272 mg m h, with an overall median of 36 mg m h. For warm conditions (air temperature of 21 ± 10°C), the highest hourly PM fluxes (range 116-146 mg m h) were observed during the early evening period, from 2000 to 2100 h. For cold conditions (air temperature of -2 ± 8°C), the highest PM fluxes (range 14-27 mg m h) were observed in the afternoon, from 1100 to 1500 h. Changes in the hourly trend of PM fluxes coincided with changes in friction velocity, air temperature, sensible heat flux, and surface roughness. The PM emission was also affected by the pen surface water content, where a water content of at least 20% (wet basis) would be sufficient to effectively reduce PM emissions from pens by as much as 60%.

  10. Highly durable superhydrophobic coatings with gradient density by movable spray method

    Science.gov (United States)

    Tenjimbayashi, Mizuki; Shiratori, Seimei

    2014-09-01

    Superhydrophobic surface is expected to be applied in anti-fouling, anti-icing, and anti-bacterial. However, practical use is interrupted by low mechanical strength, time-consuming process, and limited coating substrate. Here highly durable superhydrophobic coatings were prepared by simple and novel spraying method, which sprays with changing the "spray distance between substrate and spray" (SD), named "movable spray method." We prepared the solution that changes wettability and durability with spraying distance by mixing SiO2 nanoparticles and ethyl alpha cyanoacrylate polymer (EAC). Then, we evaluated the chemical components and surface morphologies of each spraying distance coatings (0 ˜ 50 cm) by XPS, SEM, and laser scanning microscope. It revealed that surface roughness and SiO2/EAC ratio increased as the SD increases. Thus, durable superhydrophobic coatings were designed by spraying with increasing SD gradually. Glow discharge-optical emission spectrometry analysis revealed that designed coatings showed the gradual increase of SiO2/EAC ratio. As a result, coatings prepared on glass, wood, or aluminum substrates maintained their superhydrophobicity up to the abrasion at 40 kPa. This movable spray method is simple coating by the wet process and prepares robust hydrophobic coating on complex shape and large area substrates. The gradient functional surface was found to have mechanical durability and superhydrophobicity, and wide area applications will be expected.

  11. Arbitrary magnetic field gradient waveform correction using an impulse response based pre-equalization technique.

    Science.gov (United States)

    Goora, Frédéric G; Colpitts, Bruce G; Balcom, Bruce J

    2014-01-01

    The time-varying magnetic fields used in magnetic resonance applications result in the induction of eddy currents on conductive structures in the vicinity of both the sample under investigation and the gradient coils. These eddy currents typically result in undesired degradations of image quality for MRI applications. Their ubiquitous nature has resulted in the development of various approaches to characterize and minimize their impact on image quality. This paper outlines a method that utilizes the magnetic field gradient waveform monitor method to directly measure the temporal evolution of the magnetic field gradient from a step-like input function and extracts the system impulse response. With the basic assumption that the gradient system is sufficiently linear and time invariant to permit system theory analysis, the impulse response is used to determine a pre-equalized (optimized) input waveform that provides a desired gradient response at the output of the system. An algorithm has been developed that calculates a pre-equalized waveform that may be accurately reproduced by the amplifier (is physically realizable) and accounts for system limitations including system bandwidth, amplifier slew rate capabilities, and noise inherent in the initial measurement. Significant improvements in magnetic field gradient waveform fidelity after pre-equalization have been realized and are summarized.

  12. Study of a Liquid Plug-Flow Thermal Cycling Technique Using a Temperature Gradient-Based Actuator

    Directory of Open Access Journals (Sweden)

    Yusuke Fuchiwaki

    2014-10-01

    Full Text Available Easy-to-use thermal cycling for performing rapid and small-volume DNA amplification on a single chip has attracted great interest in the area of rapid field detection of biological agents. For this purpose, as a more practical alternative to conventional continuous flow thermal cycling, liquid plug-flow thermal cycling utilizes a thermal gradient generated in a serpentine rectangular flow microchannel as an actuator. The transit time and flow speed of the plug flow varied drastically in each temperature zone due to the difference in the tension at the interface between temperature gradients. According to thermal distribution analyses in microfluidics, the plug flow allowed for a slow heating process, but a fast cooling process. The thermal cycle of the microfluid was consistent with the recommended temperature gradient for PCR. Indeed, amplification efficiency of the plug flow was superior to continuous flow PCR, and provided an impressive improvement over previously-reported flow microchannel thermal cycling techniques.

  13. Mapping axonal density and average diameter using non-monotonic time-dependent gradient-echo MRI

    Science.gov (United States)

    Nunes, Daniel; Cruz, Tomás L.; Jespersen, Sune N.; Shemesh, Noam

    2017-04-01

    White Matter (WM) microstructures, such as axonal density and average diameter, are crucial to the normal function of the Central Nervous System (CNS) as they are closely related with axonal conduction velocities. Conversely, disruptions of these microstructural features may result in severe neurological deficits, suggesting that their noninvasive mapping could be an important step towards diagnosing and following pathophysiology. Whereas diffusion based MRI methods have been proposed to map these features, they typically entail the application of powerful gradients, which are rarely available in the clinic, or extremely long acquisition schemes to extract information from parameter-intensive models. In this study, we suggest that simple and time-efficient multi-gradient-echo (MGE) MRI can be used to extract the axon density from susceptibility-driven non-monotonic decay in the time-dependent signal. We show, both theoretically and with simulations, that a non-monotonic signal decay will occur for multi-compartmental microstructures - such as axons and extra-axonal spaces, which were here used as a simple model for the microstructure - and that, for axons parallel to the main magnetic field, the axonal density can be extracted. We then experimentally demonstrate in ex-vivo rat spinal cords that its different tracts - characterized by different microstructures - can be clearly contrasted using the MGE-derived maps. When the quantitative results are compared against ground-truth histology, they reflect the axonal fraction (though with a bias, as evident from Bland-Altman analysis). As well, the extra-axonal fraction can be estimated. The results suggest that our model is oversimplified, yet at the same time evidencing a potential and usefulness of the approach to map underlying microstructures using a simple and time-efficient MRI sequence. We further show that a simple general-linear-model can predict the average axonal diameters from the four model parameters, and

  14. High-Resolution MR Imaging with Strong Local "surface" Gradient Coils, and, Optimization of Spgr Techniques for Functional MR Imaging.

    Science.gov (United States)

    Jin, Haoran

    In this thesis we discuss two specific topics in magnetic resonance imaging. The first concerns the technical requirements of high resolution MR imaging. Unique local "surface" gradient coils have been designed, constructed, integrated with a whole body MR imaging system, and used to acquire MR images demonstrating higher spatial resolution in three dimensions. The novel gradient coil design generates a strong linear gradient-field in three dimensions near the planar surface of the coil assembly for high resolution MR skin imaging. The rise times of the gradient coils were measured to be less than 250 mus, allowing rapid gradient coil switching. No significant eddy current effects have been found on the images. Images of a phantom and human skin with a field of view 3 cm by 3 cm and matrix size of 512 x 384 were obtained, corresponding to an in-plane resolution of 58 by 78 mu m. The resulting images represent a significant improvement in limiting spatial resolution compared to conventional MR images. The second topic of this thesis is functional MR imaging (FMRI). Functional MR imaging is based on the concept that neural activity in the cerebral cortex causes an increase in blood flow and a decrease in capillary deoxyhemoglobin concentrations, producing a signal enhancement in T2 ^*-weighted pulse sequences. The magnetic susceptibility of blood changes the oxygenation, changing the local T2^*. Spoiled gradient echo (SPGR) techniques both theoretically and experimentally have been optimized for functional MRI of human motor cortex. Experimental measurements have been performed and compared with the theoretical optimizations of signal to noise ratios of subtracted SPGR imaging. The experimental data are in good agreement with theoretical calculations. An FMRI of motor cortex stimulation with more than 5% intensity change has been observed using optimized techniques. Post imaging processing has been employed for displaying signal changes in the functional MR imaging.

  15. Proton acceleration using doped Argon plasma density gradient interacting with relativistic CO2 -laser pulse

    Science.gov (United States)

    Sahai, Aakash; Ettlinger, Oliver; Hicks, George; Ditter, Emma-Jane; Najmudin, Zulfikar

    2016-10-01

    We investigate proton and light-ion acceleration driven by the interaction of relativistic CO2 laser pulses with overdense Argon or other heavy-ion gas targets doped with lighter-ion species. Optically shaping the gas targets allows tuning of the pre-plasma scale-length from a few to several laser wavelengths, allowing the laser to efficiently drive a propagating snowplow through the bunching in the electron density. Preliminary PIC-based modeling shows that the lighter-ion species is accelerated even without any significant motion of the heavier ions which is a signature of the Relativistically Induced Transparency Acceleration mechanism. Some outlines of possible experiments at the TW CO2 laser at the Accelerator Test Facility at Brookhaven National Laboratory are presented.

  16. Some Bayesian statistical techniques useful in estimating frequency and density

    Science.gov (United States)

    Johnson, D.H.

    1977-01-01

    This paper presents some elementary applications of Bayesian statistics to problems faced by wildlife biologists. Bayesian confidence limits for frequency of occurrence are shown to be generally superior to classical confidence limits. Population density can be estimated from frequency data if the species is sparsely distributed relative to the size of the sample plot. For other situations, limits are developed based on the normal distribution and prior knowledge that the density is non-negative, which insures that the lower confidence limit is non-negative. Conditions are described under which Bayesian confidence limits are superior to those calculated with classical methods; examples are also given on how prior knowledge of the density can be used to sharpen inferences drawn from a new sample.

  17. Isolation of bacterial plasmids by density gradient centrifugation in cesium trifluoroacetate (CsTFA) without the use of ethidium bromide.

    Science.gov (United States)

    Andersson, K; Hjorth, R

    1985-01-01

    Plasmids extracted from bacterial cells by alkaline extraction can easily be isolated from linear DNA by isopycnic centrifugation in CsTFA. This is a fast and simple method which circumvents the use of the intercalating dye, ethidium bromide, and consequently the problems associated with its removal. The buoyant densities for covalently closed circular DNA and linear DNA in CsTFA are 1.60 g/ml and 1.65 g/ml, respectively. The isolation is achieved regardless of plasmid size and can be accomplished at temperatures of between 4 and 30 degrees C. Plasmid DNA isolated in gradients of CsTFA are of a high purity and have been found to be intact when cleaved with restriction enzymes and ligated with T4 DNA ligase.

  18. A Magnetic Resonance Measurement Technique for Rapidly Switched Gradient Magnetic Fields in a Magnetic Resonance Tomograph

    Directory of Open Access Journals (Sweden)

    K. Bartušek

    2003-01-01

    Full Text Available This paper describes a method for measuring of the gradient magnetic field in Nuclear Magnetic Resonance (NMR tomography, which is one of the modern medical diagnostic methods. A very important prerequisite for high quality imaging is a gradient magnetic field in the instrument with exactly defined properties. Nuclear magnetic resonance enables us to measure the pulse gradient magnetic field characteristics with high accuracy. These interesting precise methods were designed, realised, and tested at the Institute of Scientific Instruments (ISI of the Academy of Sciences of the Czech Republic. The first of them was the Instantaneous Frequency (IF method, which was developed into the Instantaneous Frequency of Spin Echo (IFSE and the Instantaneous Frequency of Spin Echo Series (IFSES methods. The above named methods are described in this paper and their a comparison is also presented.

  19. 3D imaging of flow patterns in an internally-pumped microfluidic device: redox magnetohydrodynamics and electrochemically-generated density gradients.

    Science.gov (United States)

    Gao, Feng; Kreidermacher, Adam; Fritsch, Ingrid; Heyes, Colin D

    2013-05-07

    Redox magnetohydrodynamics (MHD) is a promising technique for developing new electrochemical-based microfluidic flow devices with unique capabilities, such as easily switching flow direction and adjusting flow speeds and flow patterns as well as avoiding bubble formation. However, a detailed description of all the forces involved and predicting flow patterns in confined geometries is lacking. In addition to redox-MHD, density gradients caused by the redox reactions also play important roles. Flow in these devices with small fluid volumes has mainly been characterized by following microbead motion by optical microscopy either by particle tracking velocimetry (PTV) or by processing the microbead images by particle image velocimetry (PIV) software. This approach has limitations in spatial resolution and dimensionality. Here we use fluorescence correlation spectroscopy (FCS) to quantitatively and accurately measure flow speeds and patterns in the ~5-50 μm/s range in redox-MHD-based microfluidic devices, from which 3D flow maps are obtained with a spatial resolution down to 2 μm. The 2 μm spatial resolution flow speeds map revealed detailed flow profiles during redox-MHD in which the velocity increases linearly from above the electrode and reaches a plateau across the center of the cell. By combining FCS and video-microscopy (with PTV and PIV processing approaches), we are able to quantify a vertical flow of ~10 μm/s above the electrodes as a result of density gradients caused by the redox reactions and follow convection flow patterns. Overall, combining FCS, PIV, and PTV analysis of redox-MHD is a powerful combination to more thoroughly characterize the underlying forces in these promising microfluidic devices.

  20. Automatic Inspection of the Warp-Weft Density Using Image Processing Techniques

    OpenAIRE

    ALDEMİR, Erdoğan; Hakan ÖZDEMİR; KILINÇ, Selçuk

    2016-01-01

    In this study, possibility of determining warp and weft yarn density of colored and figured plain and twill woven fabrics by Wiener filter, median filter, grey level co-occurrence matrix and gray line profile methods, which are spatial techniques, and by Fourier and wavelet transformation methods, which are frequency domain techniques, are investigated. Considering the spatial techniques, the most successful technique that determines warp and weft densities of plain and twill fabrics is the m...

  1. The effects of lateral density gradients, slopes and buoyancy on channel flow: 1D analytical solutions and applications to the SE Canadian Cordillera

    Science.gov (United States)

    Gervais, Félix; Ranalli, Giorgio

    2017-08-01

    We present 1D analytical solutions for channel flow in orogens driven by various types of pressure gradients. Our calculations demonstrate that lateral density gradients in the upper crust, such as would occur across a suture zone separating arc rocks from pericratonic sediments provide a driving force for Poiseuille flow as large as topographic gradients observed in modern mountain belts. For cases for which the gradients are external (topographic and lateral density gradients) and internal (e.g. partial melting of channel material) to the channel, inclination decreases and increases the Poiseuille component of the average flow-velocity within the channel by the cosine and sine of the slope, respectively. The magnitude of the pressure gradient consequent upon the buoyancy generated by partial melting of metapelites in a channel with a 30° slope, such as would occur above an underthrusting basement ramp, is similar to that of topographic or lateral density gradients. Channel flow up a ramp could thus constitute an important exhumation mechanism in large hot orogens. Our calculations indicate that mid-crustal channel flow was a highly likely process in the Late Cretaceous-Paleocene setting of the southeastern Canadian Cordillera. The flow was first driven by the lateral density contrast between pericratonic sediments and the arc-related Intermontane terrane, then by combined effect of topographic gradient and melt-induced buoyancy of the Lower Selkirk Allochthon (part of the Shuswap Complex). Flow up the underthrusting basement ramp resulted in exhumation from mid- to upper-crustal levels. Channel flow then migrated downward to involve basement and overlying cover sequence rocks. Our results indicate that syn-convergent channel flow was a viable and very likely process in the southeastern Canadian Cordillera.

  2. Forest biomass density across large climate gradients in northern South America is related to water availability but not with temperature

    Science.gov (United States)

    Cayuela, Luis; González-Caro, Sebastián; Aldana, Ana M.; Stevenson, Pablo R.; Phillips, Oliver; Cogollo, Álvaro; Peñuela, Maria C.; von Hildebrand, Patricio; Jiménez, Eliana; Melo, Omar; Londoño-Vega, Ana Catalina; Mendoza, Irina; Velásquez, Oswaldo; Fernández, Fernando; Serna, Marcela; Velázquez-Rua, Cesar; Benítez, Doris; Rey-Benayas, José M.

    2017-01-01

    Understanding and predicting the likely response of ecosystems to climate change are crucial challenges for ecology and for conservation biology. Nowhere is this challenge greater than in the tropics as these forests store more than half the total atmospheric carbon stock in their biomass. Biomass is determined by the balance between biomass inputs (i.e., growth) and outputs (mortality). We can expect therefore that conditions that favor high growth rates, such as abundant water supply, warmth, and nutrient-rich soils will tend to correlate with high biomass stocks. Our main objective is to describe the patterns of above ground biomass (AGB) stocks across major tropical forests across climatic gradients in Northwestern South America. We gathered data from 200 plots across the region, at elevations ranging between 0 to 3400 m. We estimated AGB based on allometric equations and values for stem density, basal area, and wood density weighted by basal area at the plot-level. We used two groups of climatic variables, namely mean annual temperature and actual evapotranspiration as surrogates of environmental energy, and annual precipitation, precipitation seasonality, and water availability as surrogates of water availability. We found that AGB is more closely related to water availability variables than to energy variables. In northwest South America, water availability influences carbon stocks principally by determining stand structure, i.e. basal area. When water deficits increase in tropical forests we can expect negative impact on biomass and hence carbon storage. PMID:28301482

  3. Modelling The Effects of Density Gradients and Fluctuations on the Apparent Sizes and Positions of Low Frequency Solar Radio Sources

    Science.gov (United States)

    Alcock, Benjamin Thomas; Kontar, Eduard; Jeffrey, Natasha

    2017-08-01

    Recent high spatial and temporal resolution imaging of fast growth of the Type-III source and movement of the source centroid. In this work, we use a Monte-Carlo ray tracing simulation to model the passage of low frequency (5-240 MHz) radio waves through the solar corona from a point source, considering both isotropic and dipole emission. We model the effects of random density fluctuations and an isotropic density gradient on the transport of the rays, varying the strength of the scattering to observe the effects on images of the source from an observer at 1 AU. Absorption of photons is included, and the effects on the reproduced images and flux curves are observed. The apparent source size and centroid position are tracked through the simulation, and we find a general increase in source size with time, and a variation of centroid position in both directions throughout the simulation. We find that the size of the variation is strongly dependant upon frequency, with lower frequency sources appearing to move further on the disk than higher frequency sources. We also observe the strength of the effects at different viewing angles, finding that the greatest variation occurs closer to the solar limb. Further observational work is required to limit the scattering parameters, in order to allow for comparison with current radio images.

  4. Forest biomass density across large climate gradients in northern South America is related to water availability but not with temperature.

    Science.gov (United States)

    Álvarez-Dávila, Esteban; Cayuela, Luis; González-Caro, Sebastián; Aldana, Ana M; Stevenson, Pablo R; Phillips, Oliver; Cogollo, Álvaro; Peñuela, Maria C; von Hildebrand, Patricio; Jiménez, Eliana; Melo, Omar; Londoño-Vega, Ana Catalina; Mendoza, Irina; Velásquez, Oswaldo; Fernández, Fernando; Serna, Marcela; Velázquez-Rua, Cesar; Benítez, Doris; Rey-Benayas, José M

    2017-01-01

    Understanding and predicting the likely response of ecosystems to climate change are crucial challenges for ecology and for conservation biology. Nowhere is this challenge greater than in the tropics as these forests store more than half the total atmospheric carbon stock in their biomass. Biomass is determined by the balance between biomass inputs (i.e., growth) and outputs (mortality). We can expect therefore that conditions that favor high growth rates, such as abundant water supply, warmth, and nutrient-rich soils will tend to correlate with high biomass stocks. Our main objective is to describe the patterns of above ground biomass (AGB) stocks across major tropical forests across climatic gradients in Northwestern South America. We gathered data from 200 plots across the region, at elevations ranging between 0 to 3400 m. We estimated AGB based on allometric equations and values for stem density, basal area, and wood density weighted by basal area at the plot-level. We used two groups of climatic variables, namely mean annual temperature and actual evapotranspiration as surrogates of environmental energy, and annual precipitation, precipitation seasonality, and water availability as surrogates of water availability. We found that AGB is more closely related to water availability variables than to energy variables. In northwest South America, water availability influences carbon stocks principally by determining stand structure, i.e. basal area. When water deficits increase in tropical forests we can expect negative impact on biomass and hence carbon storage.

  5. Endothelial cell density after deep anterior lamellar keratoplasty (Melles technique)

    NARCIS (Netherlands)

    Van Dooren, BTH; Mulder, PGH; Nieuwendaal, CP; Beekhuis, WH; Melles, GRJ

    PURPOSE: To measure the recipient endothelial cell loss after the Melles technique for deep anterior lamellar keratoplasty. METHODS: In 21 eyes of 21 patients, a deep anterior lamellar keratoplasty procedure was performed. Before surgery and at 6, 12, and 24 months after surgery, specular microscopy

  6. Multiconfiguration Pair-Density Functional Theory: A Fully Translated Gradient Approximation and Its Performance for Transition Metal Dimers and the Spectroscopy of Re2Cl8(2-).

    Science.gov (United States)

    Carlson, Rebecca K; Truhlar, Donald G; Gagliardi, Laura

    2015-09-08

    We extend the on-top density functional of multiconfiguration pair-density functional theory (MC-PDFT) to include the gradient of the on-top density as well as the gradient of the density. We find that the theory is reasonably stable to this extension; furthermore, it provides improved accuracy for molecules containing transition metals. We illustrate the extended on-top density functionals by applying them to Cr2, Cu2, Ag2, Os2, and Re2Cl8(2-) as well as to our previous database of 56 data for bond dissociation energies, barrier heights, reaction energies, proton affinities, and the water dimer. The performance of MC-PDFT is comparable to or better than that of CASPT2.

  7. Energy gradients with respect to atomic positions and cell parameters for the Kohn-Sham density-functional theory at the Gamma point.

    Science.gov (United States)

    Weber, Valéry; Tymczak, Christopher J; Challacombe, Matt

    2006-06-14

    The application of theoretical methods based on density-functional theory is known to provide atomic and cell parameters in very good agreement with experimental values. Recently, construction of the exact Hartree-Fock exchange gradients with respect to atomic positions and cell parameters within the Gamma-point approximation has been introduced. In this article, the formalism is extended to the evaluation of analytical Gamma-point density-functional atomic and cell gradients. The infinite Coulomb summation is solved with an effective periodic summation of multipole tensors. While the evaluation of Coulomb and exchange-correlation gradients with respect to atomic positions are similar to those in the gas phase limit, the gradients with respect to cell parameters needs to be treated with some care. The derivative of the periodic multipole interaction tensor needs to be carefully handled in both direct and reciprocal space and the exchange-correlation energy derivative leads to a surface term that has its origin in derivatives of the integration limits that depend on the cell. As an illustration, the analytical gradients have been used in conjunction with the QUICCA algorithm to optimize one-dimensional and three-dimensional periodic systems at the density-functional theory and hybrid Hartree-Fock/density-functional theory levels. We also report the full relaxation of forsterite supercells at the B3LYP level of theory.

  8. A projected gradient method with nonmonotonic backtracking technique for solving convex constrained monotone variational inequality problem

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Based on a differentiable merit function proposed by Taji,et al in "Mathematical Programming,1993,58: 369-383",a projected gradient trust region method for the monotone variational inequality problem with convex constraints is presented.Theoretical analysis is given which proves that the proposed algorithm is globally convergent and has a local quadratic convergence rate under some reasonable conditions.The results of numerical experiments are reported to show the effectiveness of the proposed algorithm.

  9. Sucrose density gradient centrifugation and cross-flow filtration methods for the production of arbovirus antigens inactivated by binary ethylenimine

    Directory of Open Access Journals (Sweden)

    Chuan Teck F

    2004-01-01

    Full Text Available Abstract Background Sucrose density gradient centrifugation and cross-flow filtration methods have been developed and standardised for the safe and reproducible production of inactivated arbovirus antigens which are appropriate for use in diagnostic serological applications. Methods To optimise the maximum titre of growth during the propagation of arboviruses, the multiplicity of infection and choice of cell line were investigated using stocks of Ross River virus and Barmah Forest virus grown in both mosquito and mammalian cell lines. To standardise and improve the efficacy of the inactivation of arboviral suspensions, stocks of Ross River virus, Barmah Forest virus, Japanese encephalitis virus, Murray Valley encephalitis virus and Alfuy virus were chemically inactivated using binary ethylenimine at a final concentration of 3 mM. Aliquots were then taken at hourly intervals and crude inactivation rates were determined for each virus using a plaque assay. To ensure complete inactivation, the same aliquots were each passaged 3 times in Aedes albopictus C6/36 cells and the presence of viral growth was detected using an immunofluorescent assay. For larger quantities of viral suspensions, centrifugation on an isopycnic sucrose density gradient or cross-flow filtration was used to produce concentrated, pure antigens or partially concentrated, semi-purified antigens respectively. Results The results of the propagation experiments suggested that the maximum viral titres obtained for both Ross River virus and Barmah Forest virus were affected by the incubation period and choice of cell line, rather than the use of different multiplicity of infection values. Results of the binary ethylenimine inactivation trial suggested that standardised periods of 5 or 8 hours would be suitable to ensure effective and complete inactivation for a number of different arboviral antigens. Conclusion Two methods used to prepare inactivated arbovirus antigens have been

  10. Anomalous diurnal variation of atmospheric potential gradient and air-Earth current density observed at Maitri, Antarctica

    Science.gov (United States)

    Jeeva, K.; Gurubaran, S.; Williams, E. R.; Kamra, A. K.; Sinha, A. K.; Guha, A.; Selvaraj, C.; Nair, K. U.; Dhar, Ajay

    2016-11-01

    The scope of this paper is to explore the mechanisms operating over Maitri (70.76°S, 11.74°E, 117 m above mean sea level), a coastal Antarctic station, that produce an anomalous fair-weather diurnal pattern of the atmospheric electric potential gradient (PG) and air-Earth current density (AEC). The anomaly in the diurnal variations of AEC and the PG is displaying an ostensible minimum at 10 UT and a diminished response to the thunderstorm over the African continent in the 14-16 UT time frame. The data sets (2005-2014, except 2012) of the PG, and to some extent, AEC, from Maitri, are used to explore this anomaly. It follows that the fair-weather electrical phenomena over Maitri can be ascribed to global electrified convection on the one hand and to regional phenomena like convection due to the replacement of warm air by katabatic winds on the other hand. The katabatic winds originate on the polar plateau and blow from 130° at Maitri which are likely to transport various elements from the mountain slopes, and space charge from the polar plateau is expected to produce various disturbances in the PG and AEC monitored over the coastal Antarctica. This mechanism may be responsible for peaks in the early UT hours and also for the anomalous behavior of atmospheric electrical parameters observed at Maitri. Maitri data are compared with that of Carnegie cruise and Vostok to explain the source of anomaly.

  11. Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid.

    Science.gov (United States)

    Torabi, Forough; Binduraihem, Adel; Miller, David

    2017-03-01

    Mature spermatozoa bind hyaluronic acid in the extracellular matrix via hyaladherins. Immature spermatozoa may be unable to interact because they do not express the appropriate hyaladherins on their surface. Fresh human semen samples were fractionated using differential density gradient centrifugation (DDGC) and the ability of these fractions to bind hyaluronic acid was evaluated. The presence of sperm hyaladherins was also assessed. CD44 was located mainly on the acrosome and equatorial segment and became more restricted to the equatorial segment in capacitated spermatozoa. Hyaluronic acid-TRITC (hyaluronic acid conjugated with tetramethylrhodamine isothiocyanante), a generic hyaluronic-acid-binding reagent, labelled the membrane and the neck region, particularly after capacitation. Sperm populations obtained after DDGC or after interaction with hyaluronic acid were assessed for DNA fragmentation and chromatin maturity. Strong relationships between both measures and sperm sedimentation and hyaluronic-acid-binding profiles were revealed. Capacitation enhanced hyaluronic acid binding of both DDGC-pelleted sperm and sperm washed free of seminal fluid. In conclusion, hyaladherins were detected on human sperm and a higher capacity for sperm hyaluronic-acid-binding was shown to correspond with their DDGC sedimentation profiles and with lower levels of DNA fragmentation and better chromatin maturity. Capacitation induced changes in the distribution and presence of hyaladherins may enhance hyaluronic-acid-binding.

  12. PFISR GPS tracking mode for researching high-latitude ionospheric electron density gradients associated with GPS scintillation

    Science.gov (United States)

    Loucks, D. C.; Palo, S. E.; Pilinski, M.; Crowley, G.; Azeem, S. I.; Hampton, D. L.

    2016-12-01

    Ionospheric behavior in the high-latitudes can significantly impact Ultra High Frequency (UHF) signals in the 300 MHz to 3 GHz band, resulting in degradation of Global Positioning System (GPS) position solutions and satellite communications interruptions. To address these operational concerns, a need arises to identify and understand the ionospheric structure that leads to disturbed conditions in the Arctic. Structures in the high-latitude ionosphere are known to change on the order of seconds or less, can be decameters to kilometers in scale, and elongate across magnetic field lines at auroral latitudes. Nominal operations at Poker Flat Incoherent Scatter Radar (PFISR) give temporal resolution on the order of minutes, and range resolution on the order of tens of kilometers, while specialized GPS receivers available for ionospheric sensing have a 100Hz observation sampling rate. One of these, ASTRA's Connected Autonomous Space Environment Sensor (CASES) is used for this study. We have developed a new GPS scintillation tracking mode for PFISR to address open scientific questions regarding temporal and spatial electron density gradients. The mode will be described, a number of experimental campaigns will be analyzed, and results and lessons learned will be presented.

  13. Space Electron Density Gradient Studies using a 3D Embedded Reconfigurable Sounder and ESA/NASA CLUSTER Mission

    Science.gov (United States)

    Dekoulis, George

    2016-07-01

    This paper provides a direct comparison between data captured by a new embedded reconfigurable digital sounder, different ground-based ionospheric sounders spread around Europe and the ESA/NASA CLUSTER mission. The CLUSTER mission consists of four identical space probes flying in a formation that allows measurements of the electron density gradient in the local magnetic field. Both the ground-based and the spacecraft instrumentations assist in studying the motion, geometry and boundaries of the plasmasphere. The comparison results are in accordance to each other. Some slight deviations among the captured data were expected from the beginning of this investigation. These small discrepancies are reasonable and seriatim analyzed. The results of this research are significant, since the level of the plasma's ionization, which is related to the solar activity, dominates the propagation of electromagnetic waves through it. Similarly, unusually high solar activity presents serious hazards to orbiting satellites, spaceborne instrumentation, satellite communications and infrastructure located on the Earth's surface. Long-term collaborative study of the data is required to continue, in order to identify and determine the enhanced risk in advance. This would allow scientists to propose an immediate cure.

  14. First-principles local density approximation (LDA)+ U and generalized gradient approximation (GGA) + U studies of plutonium oxides

    Institute of Scientific and Technical Information of China (English)

    Sun Bo; Zhang Ping

    2008-01-01

    The electronic structures and properties of PuO2 and Pu2O3 have been studied according to the first principles by using the all-electron projector-augmented-wave (PAW) method. The local density approximation (LDA)+U and the generalized gradient approximation (GGA)+U formalisms have been used to account for the strong on-site Coulomb repulsion among the localized Pu 5f electrons. We discuss how the properties of PuO2 and Pu2O3 are affected by choosing the values of U and exchange-correlation potential. Also, the oxidation reaction of Pu2O3, leading to the formation of PuO2, and its dependence on U and exchange-correlation potential have been studied. Our results show that by choosing an appropriate U it is possible to consistently describe structural, electronic, and thermodynamic properties of PuO2 and Pu2O3, which enable the modelling of the redox process involving Pu-based materials.

  15. A long-term study on the deletion criterion of questionable electron density profiles caused by ionospheric irregularities - COSMIC radio occultation technique

    Science.gov (United States)

    Uma, G.; Brahmanandam, P. S.; Chu, Y. H.

    2016-06-01

    The crucial assumption made in the retrieval of radio-occultated atmospheric parameters is the spherical symmetry of the atmospheric refractive index, which implies that no horizontal gradient of the refractive index exists along the spherical shell. Nevertheless, the presence of density irregularities will lead to scintillation and multipath effects that often create highly fluctuating and random electron density profiles. In this study, it is proposed a reliable data quality control (QC) approach to remove questionable electron density profiles (due to the presence of ionospheric irregularities) retrieved using the COSMIC radio occultation (RO) technique based on two parameters, namely, the gradient and fluctuation of the topside density profile. Statistics of seven years density profiles (July 2006-May 2013) are presented by determining the aforementioned parameters for every density profile. The main advantage of this data QC is that it uses COSMIC RO electron density profiles retrieved from the slant total electron content (TEC) that is estimated from the excess phases of the GPS L1 and L2 frequencies only to delete the questionable profiles, instead of relying on any model and other observations. A systematic criterion has been developed based on the statistics to relinquish the so-called questionable density profiles. The computed gradients and fluctuations of the topside ionosphere electron density profiles have shown a few important features including, solar activity dependency and pronounced variations in between around +40° and -40° latitudes. After the removal of questionable profiles, both peak densities and heights of the ionosphere F layer are presented globally in different seasons of years during 2007 and 2012 that revealed several important features.

  16. Sexagem de espermatozoides bovinos por centrifugação em gradiente descontínuo de densidade de Percoll Sex selection in bovine spermatozoa by using Percoll discontinuos density gradient centrifugation

    Directory of Open Access Journals (Sweden)

    Vera Fernanda Martins Hossepian de Lima

    2011-08-01

    Full Text Available O objetivo neste trabalho foi desenvolver um método de seleção do sexo de espermatozoides bovinos por centrifugação em gradiente de densidade de Percoll. Utilizou-se sêmen congelado de touros mantidos em regime de colheita de sêmen. A fração de espermatozoides X ou Y foi separada por centrifugação em treze diferentes gradientes de densidade de Percoll formados por 1 a 12 camadas com densidades que variaram de 1,004 g/mL a 1,123 g/mL. As soluções com diferentes densidades foram preparadas misturando-se, em proporções diferentes, meio de cultura Hank's e uma solução estoque composta de NaCl 1,5 M e Percoll (1:9, v/v. Sobre cada gradiente foi colocado um total de 50 × 10(6 espermatozoides descongelados em 0,7 mL de meio Hank's e centrifugados a 250 X g por 30 minutos, em rotor horizontal, a 25°C. Os espermatozoides das frações superior e inferior foram tratados com Quinacrina Mustarda e analisados (200 deles quanto à presença do corpúsculo-F. Dos espermatozoides encontrados no sedimento de dois gradientes, compostos de 8 e 12 frações com densidades variando entre 1,050 a 1,120 g/mL e 1,044 a 1,123 g/mL, respectivamente, visualizaram-se 25% com corpúsculo-F e os 75% restantes prováveis portadores do cromossomo X. O aumento na porcentagem de espermatozoides X após a centrifugação em gradiente de densidade permitirá que esse método de sexagem seja usado em larga escala na produção comercial de carne e leite bem como no teste de progênie.The objective of this work was to develop a bovine spermatozoid sex selection method by using Percoll density gradient centrifugation. It was used frozen semen of bulls kept in semen collection regime. Fraction X or Y was separated by centrifugation in three different Percoll density gradient formed by 1 to 12 layers with densities varying from 1.004 g/mL to 1.123 g/mL. Solutions with different densities were prepared by mixing, at different proportions, Hank's culture medium and a

  17. Alternative sample-introduction technique to avoid breakthrough in gradient-elution liquid chromatography of polymers.

    Science.gov (United States)

    Reingruber, Eva; Bedani, Filippo; Buchberger, Wolfgang; Schoenmakers, Peter

    2010-10-15

    Gradient-elution liquid chromatography (GELC) is a powerful tool for the characterization of synthetic polymers. However, gradient-elution chromatograms often suffer from breakthrough phenomena. Breakthrough can be averted by using a sample solvent as weak as the mobile phase. However, this approach is only applicable to polymers for which a sufficiently strong solvent exists which is at the same time a weak eluent. Finding such a solvent for a given polymer can be laborious or may even be impossible. Besides, when working with comprehensive two-dimensional LC the effluent of the first dimension is the injection solvent of the second dimension. In this case, it is not possible to avoid breakthrough by adjusting the eluent strength of the second-dimension injection solvent. Therefore, another strategy to avert breakthrough has to be implemented. In this work, we successfully avoided breakthrough in GELC by mixing the mobile phase not before, but after the autosampler. This was demonstrated measuring a blend of poly(methyl methacrylate) standards with different molecular-weights as model mixture with comprehensive two-dimensional GELC×size-exclusion chromatography. The strategy is thought to be applicable to all substances with a sufficiently strong dependence of retention on mobile-phase composition. This typically applies to large molecules (synthetic and natural polymers) and allows efficient refocusing. Unretained and barely retained substances are not refocused and therefore suffer in the proposed setup from peak broadening. Copyright © 2010 Elsevier B.V. All rights reserved.

  18. Cultivo de células mesenquimais do sangue de cordão umbilical com e sem uso do gradiente de densidade Ficoll-Paque Blood mesenchymal stem cell culture from the umbilical cord with and without Ficoll-Paque density gradient method

    Directory of Open Access Journals (Sweden)

    Rosa Sayoko Kawasaki-Oyama

    2008-03-01

    Ficoll-Paque gradient density method (d=1.077g/ml. METHODS: Ten samples of the umbilical cord blood obtained from full-term deliveries were submitted to two different procedures of mesenchymal stem cell culture: a Method without the Ficoll-Paque density gradient, which concentrates all nucleated cells; b Method with the Ficoll-Paque density gradient, which selects only low-density mononuclear cells. Cells were initially plated into 25 cm² cultures flasks at a density of 1x10(7 nucleated cells/cm² and 1x10(6 mononuclear cells/cm². RESULTS: It was obtained 2-13x10(7 (median = 2.35x10(7 nucleated cells/cm² by the method without the Ficoll-Paque gradient density, and 3.7-15.7x10(6 (median = 7.2x10(6 mononuclear cells/cm² by the method with the Ficoll-Paque gradient density. In all cultures adherent cells were observed 24 hours after being cultured. Cells presented fibroblastoid and epithelioid morphology. In most of the cultures, cell proliferation occurred in the first week, but after the second week only some cultures - derived from the method without the Ficoll-Paque gradient density - maintained the growth rate reaching confluence. Those cultures were submitted to trypsinization with 0.25% trypsin/EDTA solution and cultured for two to three months. CONCLUSION: In the samples analyzed, cell separation and mesenchymal stem cell culture techniques from human umbilical cord blood by the method without the Ficoll-Paque density gradient was more efficient than the method with the Ficoll-Paque density gradient.

  19. Evaluation of Fat Suppression of Diffusion-weighted Imaging Using Section Select Gradient Reversal Technique on 3 T Breast MRI.

    Science.gov (United States)

    Takemori, Daichi; Kimura, Daisuke; Yamada, Eiji; Higashida, Mitsuji

    2016-07-01

    This study evaluates fat suppression of diffusion-weighted imaging (DWI) using section select gradient reversal (SSGR) technique in clinical images on 3 T breast MRI. A total of 20 patients with breast cancer were examined at a Philips Ingenia 3 T MRI. We acquired DWI with SPAIR, SSGR-SPAIR, STIR, and SSGR-STIR. We evaluated contrast between the fat region and lesion, the coefficient of variance (CV) of the fat region and the apparent diffusion coefficient (ADC) of normal breast tissue and lesion. The contrast between the fat region and lesion was improved with SSGR technique. The CV of the fattest region did not have any significant difference in SPAIR technique (p>0.05), but it was significantly decreased in the STIR technique using SSGR technique (p<0.05). Positive correlation was observed in ADC value between SPAIR and other fat suppression techniques (SSGR-SPAIR, STIR, SSGR-STIR). DWI using SSGR technique was suggested to be effective on 3 T breast MRI.

  20. Performance improvement of n-i-p μc-Si:H solar cells by gradient hydrogen dilution technique

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    High pressure radio frequency plasma enhanced chemical vapor deposition(RF-PECVD)process was adopted to investigate the effect of constant hydrogen dilution technique and gradient hydrogen dilu-tion technique on the structural evolution of intrinsic films and the performance of n-i-p microcrystal-line silicon solar cells.The experiment results demonstrated that the grain size and crystalline volume fraction along the growth direction of intrinsic films can be controlled and the performance of solar cells can be greatly improved by gradient hydrogen dilution technique.An initial active-area efficiency of 5.7%(Voc=0.47V,Jsc=20.2mA/cm2,FF=60%)for the μc-Si:H single-junction n-i-p solar cells and an initial active-area efficiency of 10.12%(Voc=1.2V,Jsc=12.05mA/cm2,FF=70%)for the a-Si:H/μc-Si:H tandem n-i-p solar cells has been achieved.

  1. Analysis of the Nucleoside Content of Cordyceps sinensis Using the Stepwise Gradient Elution Technique of Thin-Layer Chromatography

    Institute of Scientific and Technical Information of China (English)

    MA,King-Wah(马敬桦); CHAU,Foo-Tim(周福添); WU,Jian-Yong(吴建勇)

    2004-01-01

    Nucleoside is the main class of active components in Cordyceps sinensis. Thin-layer chromatography (TLC) is one of the most commonly used methods in pharmacopoeias for analyzing chemical components of herbal medicine. Since the isocratic elution method cannot be applied successfully in TLC analysis for separating all the nucleoside components, the stepwise gradient elution has been developed in this work to separate eight nucleoside standards with success. In this way, quantitative analyses of the samples of Cordyceps sinensis were achieved via the proposed TLC procedure coupled with the scanning densitometric techniques of CAMAG and TLCQA methods for qualitative and quantitative analysis.

  2. Numerical simulation of the growth of 2″ diameter GaAs crystals by the vertical gradient freeze technique

    Science.gov (United States)

    Weimann, H.; Amon, J.; Jung, Th.; Müller, G.

    1997-10-01

    We present a global two-dimensional model of a multi-zone-furnace for the growth of GaAs using the bottom-seeded vertical gradient freeze (VGF) technique. The finite element code FIDAP was used to perform calculations of the heat transfer due to radiation and conduction in the whole furnace. The numerical results show a good agreement between measured and calculated temperature distributions in the furnace and calculated/measured power consumptions of the heaters. Quasi-steady-state calculations for a typical growth process were performed and the influence of different growth velocities on the interface shape was analyzed.

  3. X-ray radiographic technique for measuring density uniformity of silica aerogel

    CERN Document Server

    Tabata, Makoto; Adachi, Ichiro; Morita, Takeshi; Nishikawa, Keiko; 10.1016/j.nima.2012.09.001

    2012-01-01

    This paper proposes a new X-ray radiographic technique for measuring density uniformity of silica aerogels used as radiator in proximity-focusing ring-imaging Cherenkov detectors. To obtain high performance in a large-area detector, a key characteristic of radiator is the density (i.e. refractive index) uniformity of an individual aerogel monolith. At a refractive index of n = 1.05, our requirement for the refractive index uniformity in the transverse plane direction of an aerogel tile is |\\delta (n - 1)/(n - 1)| < 4% in a focusing dual layer radiator (with different refractive indices) scheme. We applied the radiographic technique to evaluate the density uniformity of our original aerogels from a trial production and that of Panasonic products (SP-50) as a reference, and to confirm they have sufficient density uniformity within \\pm 1% along the transverse plane direction. The measurement results show that the proposed technique can quantitatively estimate the density uniformity of aerogels.

  4. Influence of plasma density and plasma sheath dynamics on the ion implantation by plasma immersion technique

    OpenAIRE

    Ensinger, Wolfgang

    1996-01-01

    Influence of plasma density and plasma sheath dynamics on the ion implantation by plasma immersion technique / B. Rauschenbach ... - In: Nuclear instruments and methods in physics research. B. 113. 1996. S. 266-269

  5. Relativistically Induced Transparency Acceleration (RITA) of Protons and Light-ions with Ultrashort Laser Interaction with Heavy-ion Plasma Density Gradient

    CERN Document Server

    Sahai, Aakash A; Tableman, A R; Mori, W B; Katsouleas, T C

    2014-01-01

    The relativistically induced transparency acceleration (RITA) scheme of proton and ion acceleration using laser-plasma interactions is introduced, modeled, and compared to the existing schemes. Protons are accelerated with femtosecond relativistic pulses to produce quasimonoenergetic bunches with controllable peak energy. The RITA scheme works by a relativistic laser inducing transparency to densities higher than the cold-electron critical density, while the background heavy ions are stationary. The rising laser pulse creates a traveling acceleration structure at the relativistic critical density by ponderomotively driving a local electron density inflation, creating an electron snowplow and a co-propagating electrostatic potential. The snowplow advances with a velocity determined by the rate of the rise of the laser's intensity envelope and the heavy-ion-plasma density gradient scale length. The rising laser is incrementally rendered transparent to higher densities such that the relativistic-electron plasma ...

  6. Advancing biodiversity-ecosystem functioning science using high-density tree-based experiments over functional diversity gradients.

    Science.gov (United States)

    Tobner, Cornelia M; Paquette, Alain; Reich, Peter B; Gravel, Dominique; Messier, Christian

    2014-03-01

    Increasing concern about loss of biodiversity and its effects on ecosystem functioning has triggered a series of manipulative experiments worldwide, which have demonstrated a general trend for ecosystem functioning to increase with diversity. General mechanisms proposed to explain diversity effects include complementary resource use and invoke a key role for species' functional traits. The actual mechanisms by which complementary resource use occurs remain, however, poorly understood, as well as whether they apply to tree-dominated ecosystems. Here we present an experimental approach offering multiple innovative aspects to the field of biodiversity-ecosystem functioning (BEF) research. The International Diversity Experiment Network with Trees (IDENT) allows research to be conducted at several hierarchical levels within individuals, neighborhoods, and communities. The network investigates questions related to intraspecific trait variation, complementarity, and environmental stress. The goal of IDENT is to identify some of the mechanisms through which individuals and species interact to promote coexistence and the complementary use of resources. IDENT includes several implemented and planned sites in North America and Europe, and uses a replicated design of high-density tree plots of fixed species-richness levels varying in functional diversity (FD). The design reduces the space and time needed for trees to interact allowing a thorough set of mixtures varying over different diversity gradients (specific, functional, phylogenetic) and environmental conditions (e.g., water stress) to be tested in the field. The intention of this paper is to share the experience in designing FD-focused BEF experiments with trees, to favor collaborations and expand the network to different conditions.

  7. A technique for studying polymer interactions with concentration gradients using light scattering and viscometry

    Science.gov (United States)

    Sorci, Gina Aline

    Light scattering together with viscometry and chromatography techniques are used to examine polymers and their interactions with various substances such as salts and surfactants. New and improved upon techniques provide detailed results which have never been possible before. One such technique is developed which uses a programmable mixer to study interactions of polymers over a wide range of concentrations. It is referred to as the automatic continuous mixing technique or simply ACM. This technique has led to detailed studies of virial coefficients and the radius of gyration of polyelectrolytes with several different salts. These studies have led to verification of several theoretical models in which the computations match the experimental results. One such significant finding is the relationship between A 2 and A3. It was predicted assuming the polymers to be spheres that the relationship be A3 ∝ A22 . This remarkable relationship was seen in the experimental results for Hyaluronate in NaCl. This technique was also used to study a variety of different interactions such as surfactants and polymers, zwitterion polymers and salts as well as more complex systems of proteins and denaturing agents. Light scattering is also implemented in the HTDSLS which is heterogeneous time dependent static light scattering and ACOMP automatic continuous on-line monitoring of polymerization reactions. HTDSLS enables one to monitor simultaneously a polymer solution contaminated with larger particles provided certain conditions are meant. The technique was used to observe the simultaneous growth of virus (polymer) and bacteria (large particle). The ACOMP technique which has been successfully implemented for a variety of polymerizations is used here to monitor several different types of polymerizations simple radical growth. ATRP (atom transfer radical polymerization) as well as the step growth of polyamine. All of these utilizations of light scattering have significant applications to

  8. Quantitative Analysis of Diffusion Weighted MR Images of Brain Tumor Using Signal Intensity Gradient Technique

    Directory of Open Access Journals (Sweden)

    S. S. Shanbhag

    2014-01-01

    Full Text Available The purpose of this study was to evaluate the role of diffusion weighted-magnetic resonance imaging (DW-MRI in the examination and classification of brain tumors, namely, glioma and meningioma. Our hypothesis was that as signal intensity variations on diffusion weighted (DW images depend on histology and cellularity of the tumor, analysing the signal intensity characteristics on DW images may allow differentiating between the tumor types. Towards this end the signal intensity variations on DW images of the entire tumor volume data of 20 subjects with glioma and 12 subjects with meningioma were investigated and quantified using signal intensity gradient (SIG parameter. The relative increase in the SIG values (RSIG for the subjects with glioma and meningioma was in the range of 10.08–28.36 times and 5.60–9.86 times, respectively, compared to their corresponding SIG values on the contralateral hemisphere. The RSIG values were significantly different between the subjects with glioma and meningioma (P<0.01, with no overlap between RSIG values across the two tumors. The results indicate that the quantitative changes in the RSIG values could be applied in the differential diagnosis of glioma and meningioma, and their adoption in clinical diagnosis and treatment could be helpful and informative.

  9. Direct gradient projection method with transformation of variables technique for structural topology optimization

    NARCIS (Netherlands)

    Chang, C.; Borgart, A.; Chen, A.; Hendriks, M.A.N.

    2014-01-01

    This paper proposes an efficient and reliable topology optimization method that can obtain a black and white solution with a low objective function value within a few tens of iterations. First of all, a transformation of variables technique is adopted to eliminate the constraints on the design varia

  10. Long-term density evolution through semi-analytical and differential algebra techniques

    Science.gov (United States)

    Wittig, Alexander; Colombo, Camilla; Armellin, Roberto

    2017-08-01

    This paper introduces and combines for the first time two techniques to allow long-term density propagation in astrodynamics. First, we introduce an efficient method for the propagation of phase space densities based on differential algebra (DA) techniques. Second, this DA density propagator is used in combination with a DA implementation of the averaged orbital dynamics through semi-analytical methods. This approach combines the power of orbit averaging with the efficiency of DA techniques. While the DA-based method for the propagation of densities introduced in this paper is independent of the dynamical system under consideration, the particular combination of DA techniques with averaged equations of motion yields a fast and accurate technique to propagate large clouds of initial conditions and their associated probability density functions very efficiently for long time. This enables the study of the long-term behavior of particles subjected to the given dynamics. To demonstrate the effectiveness of the proposed approach, the evolution of a cloud of high area-to-mass objects in Medium Earth Orbit is reproduced considering the effects of solar radiation pressure, the Earth's oblateness and luni-solar perturbations. The method can propagate 10,000 random fragments and their density for 1 year within a few seconds on a common desktop PC.

  11. Average OH density in alternating current dielectric barrier discharge by laser-induced fluorescence technique

    Science.gov (United States)

    Yang, Hongliang; Feng, Chunlei; Gao, Liang; Ding, Hongbin

    2015-10-01

    The average OH density in atmospheric He-H2O(0.4%) needle-plate dielectric barrier discharge (DBD) was measured by the asynchronous laser-induced fluorescence (LIF) technique and the fluctuation of OH radical density was measured simultaneously to prove that the average OH density can be obtained by the asynchronous LIF technique. The evolution of the average OH density in four different discharge patterns, namely, negative barrier corona discharge, glow discharge, multi glow discharge, and streamer discharge, was studied, and it was found that the average OH density has an observable increase from corona discharge to streamer discharge. The main mechanism of OH production in the four different discharge patterns was analyzed. It was shown that the main mechanism of OH production in negative barrier corona discharge is electron direct collision dissociation, whereas in the other three discharge patterns the He metastable Penning ionization is the main process.

  12. Study of density field measurement based on NPLS technique in supersonic flow

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Due to the influence of shock wave and turbulence, supersonic density field exhibits strongly inhomogeneous and unsteady characteristics. Applying traditional density field measurement techniques to supersonic flows yields three problems: low spatiotemporal resolution, limitation of measuring 3D density field, and low signal to noise ratio (SNR). A new method based on Nano-based Planar Laser Scattering (NPLS) technique is proposed in this paper to measure supersonic density field. This method measures planar transient density field in 3D supersonic flow by calibrating the relationship between density and concentration of tracer particles, which would display the density fluctuation due to the influence of shock waves and vortexes. The application of this new method to density field measurement of supersonic optical bow cap is introduced in this paper, and the results reveal shock wave, turbulent boundary layer in the flow with the spatial resolution of 93.2 μm/pixel. By analyzing the results at interval of 5 μs, temporal evolution of density field can be observed.

  13. Study of density field measurement based on NPLS technique in supersonic flow

    Institute of Scientific and Technical Information of China (English)

    TIAN LiFeng; YI ShiHe; ZHAO YuXin; HE Lin; CHENG ZhongYu

    2009-01-01

    Due to the influence of shock wave and turbulence,supersonic density field exhibits strongly inho-mogeneous and unsteady characteristics.Applying traditional density field measurement techniques to supersonic flows yields three problems: low spatiotemporal resolution,limitation of measuring 3D density field,and low signal to noise ratio (SNR).A new method based on Nano-based Planar Laser Scattering (NPLS) technique is proposed in this paper to measure supersonic density field.This method measures planar transient density field in 3D supersonic flow by calibrating the relationship between density and concentration of tracer particles,which would display the density fluctuation due to the influence of shock waves and vortexes.The application of this new method to density field measurement of supersonic optical bow cap is introduced in this paper,and the results reveal shock wave,turbulent boundary layer in the flow with the spatial resolution of 93.2 pm/pixel.By analyzing the results at interval of 5 μs,temporal evolution of density field can be observed.

  14. High energy X-ray diffraction study of a dental ceramics–titanium functional gradient material prepared by field assisted sintering technique

    Energy Technology Data Exchange (ETDEWEB)

    Witte, K., E-mail: kerstin.witte@uni-rostock.de [Institute of Physics, University of Rostock, August-Bebel-Str. 55, 18055 Rostock (Germany); Bodnar, W. [Institute of Physics, University of Rostock, August-Bebel-Str. 55, 18055 Rostock (Germany); Schell, N. [Institute of Materials Research, Helmholtz-Center Geesthacht, Max-Planck-Str. 1, 21502 Geesthacht (Germany); Lang, H. [Department of Operative Dentistry and Periodontology, University of Rostock, Strempelstr. 13, 18057 Rostock (Germany); Burkel, E. [Institute of Physics, University of Rostock, August-Bebel-Str. 55, 18055 Rostock (Germany)

    2014-09-15

    A functional gradient material with eleven layers composed of a dental ceramics and titanium was successfully consolidated using field assisted sintering technique in a two-step sintering process. High energy X-ray diffraction studies on the gradient were performed at High Energy Material Science beamline at Desy in Hamburg. Phase composition, crystal unit edges and lattice mismatch along the gradient were determined applying Rietveld refinement procedure. Phase analysis revealed that the main crystalline phase present in the gradient is α-Ti. Crystallinity increases stepwisely along the gradient with a decreasing increment between every next layer, following rather the weight fraction of titanium. The crystal unit edge a of titanium remains approximately constant with a value of 2.9686(1) Å, while c is reduced with increasing amount of titanium. In the layer with pure titanium the crystal unit edge c is constant with a value of 4.7174(2) Å. The lattice mismatch leading to an internal stress was calculated over the whole gradient. It was found that the maximal internal stress in titanium embedded in the studied gradient is significantly smaller than its yield strength, which implies that the structure of titanium along the whole gradient is mechanically stable. - Highlights: • High energy XRD studies of dental ceramics–Ti gradient material consolidated by FAST. • Phase composition, crystallinity and lattice parameters are determined. • Crystallinity increases stepwisely along the gradient following weight fraction of Ti. • Lattice mismatch leading to internal stress is calculated over the whole gradient. • Internal stress in α-Ti embedded in the gradient is smaller than its yield strength.

  15. TSP, PM10, and PM2.5 emissions from a beef cattle feedlot using the flux-gradient technique

    Science.gov (United States)

    Bonifacio, Henry F.; Maghirang, Ronaldo G.; Trabue, Steven L.; McConnell, Laura L.; Prueger, John H.; Bonifacio, Edna R.

    2015-01-01

    Emissions data on air pollutants from large open-lot beef cattle feedlots are limited. This research was conducted to determine emissions of total suspended particulates (TSP) and particulate matter (PM10 and PM2.5) from a commercial beef cattle feedlot in Kansas (USA). Vertical particulate concentration profiles at the feedlot were measured using gravimetric samplers, and micrometeorological parameters were monitored with eddy covariance instrumentation during the nine 4- to 5-day intensive sampling campaigns from May 2010 through September 2011. Emission fluxes were determined from the measured concentration gradients and meteorological parameters using the flux-gradient technique. PM ratios based on calculated emission fluxes were 0.28 for PM2.5/PM10, 0.12 for PM2.5/TSP, and 0.24 for PM10/TSP, indicating that a large fraction of the PM emitted at the studied feedlot was in the coarse range of aerodynamic diameter, >10 μm. Median daily emission factors were 57, 21, and 11 kg 1000-head (hd)-1 d-1 for TSP (n = 20 days), PM10 (n = 19 days), and PM2.5 (n = 11 days), respectively. Cattle pen surface moisture contents of at least 20-30% significantly reduced both TSP and PM10 emissions, but moisture's effect on PM2.5 emissions was not established due to difficulty in measuring PM2.5 concentrations under low-PM conditions.

  16. Gradient enhanced-fluidity liquid hydrophilic interaction chromatography of ribonucleic acid nucleosides and nucleotides: A "green" technique.

    Science.gov (United States)

    Beilke, Michael C; Beres, Martin J; Olesik, Susan V

    2016-03-04

    A "green" hydrophilic interaction liquid chromatography (HILIC) technique for separating the components of mixtures with a broad range of polarities is illustrated using enhanced-fluidity liquid mobile phases. Enhanced-fluidity liquid chromatography (EFLC) involves the addition of liquid CO2 to conventional liquid mobile phases. Decreased mobile phase viscosity and increased analyte diffusivity results when a liquefied gas is dissolved in common liquid mobile phases. The impact of CO2 addition to a methanol:water (MeOH:H2O) mobile phase was studied to optimize HILIC gradient conditions. For the first time a fast separation of 16 ribonucleic acid (RNA) nucleosides/nucleotides was achieved (16min) with greater than 1.3 resolution for all analyte pairs. By using a gradient, the analysis time was reduced by over 100% compared to similar separations conducted under isocratic conditions. The optimal separation using MeOH:H2O:CO2 mobile phases was compared to MeOH:H2O and acetonitrile:water (ACN:H2O) mobile phases. Based on chromatographic performance parameters (efficiency, resolution and speed of analysis) and an assessment of the environmental impact of the mobile phase mixtures, MeOH:H2O:CO2 mixtures are preferred over ACN:H2O or MeOH:H2O mobile phases for the separation of mixtures of RNA nucleosides and nucleotides.

  17. Comparative assessment of density functional methods for evaluating essential parameters to simulate SERS spectra within the excited state energy gradient approximation

    Science.gov (United States)

    Mohammadpour, Mozhdeh; Jamshidi, Zahra

    2016-05-01

    The prospect of challenges in reproducing and interpretation of resonance Raman properties of molecules interacting with metal clusters has prompted the present research initiative. Resonance Raman spectra based on the time-dependent gradient approximation are examined in the framework of density functional theory using different methods for representing the exchange-correlation functional. In this work the performance of different XC functionals in the prediction of ground state properties, excitation state energies, and gradients are compared and discussed. Resonance Raman properties based on time-dependent gradient approximation for the strongly low-lying charge transfer states are calculated and compared for different methods. We draw the following conclusions: (1) for calculating the binding energy and ground state geometry, dispersion-corrected functionals give the best performance in comparison to ab initio calculations, (2) GGA and meta GGA functionals give good accuracy in calculating vibrational frequencies, (3) excited state energies determined by hybrid and range-separated hybrid functionals are in good agreement with EOM-CCSD calculations, and (4) in calculating resonance Raman properties GGA functionals give good and reasonable performance in comparison to the experiment; however, calculating the excited state gradient by using the hybrid functional on the hessian of GGA improves the results of the hybrid functional significantly. Finally, we conclude that the agreement of charge-transfer surface enhanced resonance Raman spectra with experiment is improved significantly by using the excited state gradient approximation.

  18. Development of a vector-tensor system to measure the absolute magnetic flux density and its gradient in magnetically shielded rooms.

    Science.gov (United States)

    Voigt, J; Knappe-Grüneberg, S; Gutkelch, D; Haueisen, J; Neuber, S; Schnabel, A; Burghoff, M

    2015-05-01

    Several experiments in fundamental physics demand an environment of very low, homogeneous, and stable magnetic fields. For the magnetic characterization of such environments, we present a portable SQUID system that measures the absolute magnetic flux density vector and the gradient tensor. This vector-tensor system contains 13 integrated low-critical temperature (LTc) superconducting quantum interference devices (SQUIDs) inside a small cylindrical liquid helium Dewar with a height of 31 cm and 37 cm in diameter. The achievable resolution depends on the flux density of the field under investigation and its temporal drift. Inside a seven-layer mu-metal shield, an accuracy better than ±23 pT for the components of the static magnetic field vector and ±2 pT/cm for each of the nine components of the gradient tensor is reached by using the shifting method.

  19. Diffusive gradient in thin films technique for assessment of cadmium and copper bioaccessibility to radish (Raphanus sativus).

    Science.gov (United States)

    Dočekalová, Hana; Škarpa, Petr; Dočekal, Bohumil

    2015-03-01

    The aim of this study was to assess cadmium and copper uptake by radish (Raphanus sativus) and to test the capability of the diffusive gradient in thin films (DGT) technique to predict bioaccessibility of the metals for this plant. Radish plants were grown in pots filled with uncontaminated control and artificially contaminated soils differing in cadmium and copper contents. Metal concentrations in plants were compared with free ion metal concentrations in soil solution, and concentrations measured by DGT. Significant correlation was found between metal fluxes to plant and metal fluxes into DGT. Pearson correlation coefficient for cadmium was 0.994 and for copper 0.998. The obtained results showed that DGT offers the possibility of simple test procedure for soils and can be used as a physical surrogate for plant uptake.

  20. Breast density mapping based upon system calibration, x-ray techniques, and FFDM images

    Science.gov (United States)

    Chen, Biao; Smith, Andrew P.; Jing, Zhenxue; Wu, Tao

    2007-03-01

    Clinical studies have correlated a high breast density to a women's risk of breast cancer. A breast density measurement that can quantitatively depict the volume distribution and percentage of dense tissues in breasts would be very useful for risk factor assessment of breast cancer, and might be more predictive of risks than the common but subjective and coarse 4-point BIRADS scale. This paper proposes to use a neural-network mapping to compute the breast density information based upon system calibration data, x-ray techniques, and Full Field Digital Mammography (FFDM) images. The mapping consists of four modules, namely, system calibration, generator of beam quality, generator of normalized absorption, and a multi-layer feed-forward neural network. As the core of breast density mapping, the network accepts x-ray target/filter combination, normalized x-ray absorption, pixel-wise breast thickness map, and x-ray beam quality during image acquisition as input elements, and exports a pixel-wise breast density distribution and a single breast density percentage for the imaged breast. Training and testing data sets for the design and verification of the network were formulated from calibrated x-ray beam quality, imaging data with a step wedge phantom under a variety x-ray imaging techniques, and nominal breast densities of tissue equivalent materials. The network was trained using a Levenberg-Marquardt algorithm based back-propagation learning method. Various thickness and glandular density phantom studies were performed with clinical x-ray techniques. Preliminary results showed that the neural network mapping is promising in accurately computing glandular density distribution and breast density percentage.

  1. A Breast Cell Atlas: Organelle analysis of the MDA-MB-231 cell line by density-gradient fractionation using isotopic marking and label-free analysis

    Directory of Open Access Journals (Sweden)

    Marianne Sandin

    2015-09-01

    Full Text Available Protein translocation between organelles in the cell is an important process that regulates many cellular functions. However, organelles can rarely be isolated to purity so several methods have been developed to analyse the fractions obtained by density gradient centrifugation. We present an analysis of the distribution of proteins amongst organelles in the human breast cell line, MDA-MB-231 using two approaches: an isotopic labelling and a label-free approach.

  2. A protocol for exosome isolation and characterization: evaluation of ultracentrifugation, density-gradient separation, and immunoaffinity capture methods.

    Science.gov (United States)

    Greening, David W; Xu, Rong; Ji, Hong; Tauro, Bow J; Simpson, Richard J

    2015-01-01

    Exosomes are 40-150 nm extracellular vesicles that are released from a multitude of cell types, and perform diverse cellular functions including intercellular communication, antigen presentation, and transfer of tumorigenic proteins, mRNA and miRNA. Exosomes are important regulators of the cellular niche, and their altered characteristics in many diseases, such as cancer, suggest their importance for diagnostic and therapeutic applications, and as drug delivery vehicles. Exosomes have been purified from biological fluids and in vitro cell cultures using a variety of strategies and techniques. In this chapter, we reveal the protocol and key insights into the isolation, purification and characterization of exosomes, distinct from shed microvesicles and apoptotic blebs. Using the colorectal cancer cell line LIM1863 as a cell model, a comprehensive evaluation of exosome isolation methods including ultracentrifugation (UC-Exos), OptiPrep™ density-based separation (DG-Exos), and immunoaffinity capture using anti-EpCAM-coated magnetic beads (IAC-Exos) were examined. All exosome isolation methodologies contained 40-150 nm vesicles based on electron microscopy, and positive for exosome markers (Alix, TSG101, HSP70) based on immunoblotting. This protocol employed a proteomic profiling approach to characterize the protein composition of exosomes, and label-free spectral counting to evaluate the effectiveness of each method in exosome isolation. Based on the number of MS/MS spectra identified for exosome markers and proteins associated with their biogenesis, trafficking, and release, IAC-Exos was shown to be the most effective method to isolate exosomes. However, the use of density-based separation (DG-Exos) provides significant advantages for exosome isolation when the use of immunoaffinity capture is limited (due to antibody availability and suitability of exosome markers).

  3. Preparation and characterization of chemical gradient surfaces and their application for the study of cellular interaction phenomena

    NARCIS (Netherlands)

    Ruardy, TG; Schakenraad, JM; vanderMei, HC; Busscher, HJ

    1997-01-01

    Chemical gradient surfaces are surfaces with a gradually changing chemistry along their length which is responsible for a position bound variation in physical properties, most notably, the wettability. In this review, methods to prepare (palladium deposition, diffusion technique, density gradient

  4. On the formation of sharp gradients in electron density resulting from an ice-plasma feedback instabilities in the polar summer mesosphere

    Science.gov (United States)

    Yee, J.; Bahcivan, H.

    2014-12-01

    Polar Mesospheric Summer Echoes (PMSEs) have commonly been attributed to scattering from (1) volume-filled electron density turbulence and (2) extremely sharp (meter-scale) gradients in electron density. In-situ sounding rockets have measured both extended regions of turbulence as well as electron density ledges. Unlike the turbulence theory, which has been extensively studied, a theory on the formation of sharp edges has yet to be explored. In this study, we reconsider the study of Gumbel et al. [2002] on the influences of ice particles on the ion chemistry and propose a theory of ice-plasma feedback instability in order to explain the origin of sharp electron density gradients. A one-dimensional particle-in-cell simulation of a multi-constituent weakly-ionized plasma has been developed to capture the physics of proton-hydrates (H+[H20]n), ice, and plasma interactions on a spatial grid of approximately 25 m. The simulation captures (1) the development of the proton-hydrate chain starting at n=4 via Thomson's model and Natanson's recombination scheme, (2) the formation of ice nucleation on large cluster proton-hydrates (n > 73), (3) the attachment of electrons (and ions) to ice particles, and resulting depletion of the plasma density, which significantly accelerates the proton-hydrate chain and ionic nucleation of new ice particles, (4) and the development of an ice-plasma feedback instability whereby the repeated process of developing new ice particles leads to capture of even more electrons and further depletion of existing regions of lower plasma density. Since this feedback instability process is faster than typical aerosol diffusion rates in this region, existing gradients steepen to produce electron density ledges. Although ionic nucleation is not feasible as the major mesospheric nucleation process, it can become efficient locally, near the coldest parts of the mesosphere because the proton-hydrate chain development is extremely sensitive to ambient

  5. Determination of free Zn2+ concentration in synthetic and natural samples with AGNES (Absence of Gradients and Nernstian Equilibrium Stripping) and DMT (Donnan Membrane Techniques)

    NARCIS (Netherlands)

    Chito, D.; Weng, L.P.; Galceran, J.; Companys, E.; Puy, J.; Riemsdijk, van W.H.; Leeuwen, van H.P.

    2012-01-01

    The determination of free Zn2+ ion concentration is a key in the study of environmental systems like river water and soils, due to its impact on bioavailability and toxicity. AGNES (Absence of Gradients and Nernstian Equilibrium Stripping) and DMT (Donnan Membrane Technique) are emerging techniques

  6. Molecular Rayleigh Scattering Techniques Developed for Measuring Gas Flow Velocity, Density, Temperature, and Turbulence

    Science.gov (United States)

    Mielke, Amy F.; Seasholtz, Richard G.; Elam, Kristie A.; Panda, Jayanta

    2005-01-01

    Nonintrusive optical point-wise measurement techniques utilizing the principles of molecular Rayleigh scattering have been developed at the NASA Glenn Research Center to obtain time-averaged information about gas velocity, density, temperature, and turbulence, or dynamic information about gas velocity and density in unseeded flows. These techniques enable measurements that are necessary for validating computational fluid dynamics (CFD) and computational aeroacoustic (CAA) codes. Dynamic measurements allow the calculation of power spectra for the various flow properties. This type of information is currently being used in jet noise studies, correlating sound pressure fluctuations with velocity and density fluctuations to determine noise sources in jets. These nonintrusive techniques are particularly useful in supersonic flows, where seeding the flow with particles is not an option, and where the environment is too harsh for hot-wire measurements.

  7. Sodium MRI using a density-adapted 3D radial acquisition technique.

    Science.gov (United States)

    Nagel, Armin M; Laun, Frederik B; Weber, Marc-André; Matthies, Christian; Semmler, Wolfhard; Schad, Lothar R

    2009-12-01

    A density-adapted three-dimensional radial projection reconstruction pulse sequence is presented which provides a more efficient k-space sampling than conventional three-dimensional projection reconstruction sequences. The gradients of the density-adapted three-dimensional radial projection reconstruction pulse sequence are designed such that the averaged sampling density in each spherical shell of k-space is constant. Due to hardware restrictions, an inner sphere of k-space is sampled without density adaption. This approach benefits from both the straightforward handling of conventional three-dimensional projection reconstruction sequence trajectories and an enhanced signal-to-noise ratio (SNR) efficiency akin to the commonly used three-dimensional twisted projection imaging trajectories. Benefits for low SNR applications, when compared to conventional three-dimensional projection reconstruction sequences, are demonstrated with the example of sodium imaging. In simulations of the point-spread function, the SNR of small objects is increased by a factor 1.66 for the density-adapted three-dimensional radial projection reconstruction pulse sequence sequence. Using analytical and experimental phantoms, it is shown that the density-adapted three-dimensional radial projection reconstruction pulse sequence allows higher resolutions and is more robust in the presence of field inhomogeneities. High-quality in vivo images of the healthy human leg muscle and the healthy human brain are acquired. For equivalent scan times, the SNR is up to a factor of 1.8 higher and anatomic details are better resolved using density-adapted three-dimensional radial projection reconstruction pulse sequence. (c) 2009 Wiley-Liss, Inc.

  8. Effect of Biofilm on Passive Sampling of Dissolved Orthophosphate Using the Diffusive Gradients in Thin Films Technique.

    Science.gov (United States)

    Feng, Zhongmin; Zhu, Peng; Fan, Hongtao; Piao, Shanshan; Xu, Liang; Sun, Ting

    2016-07-05

    We evaluated the possibility of sampling dissolved orthophosphate using the diffusive gradient in thin films (DGT) technique with a phosphate ion-imprinted polymer (PIP)-based adsorbent and assessed the effect of biofilm on the DGT measurement. The composition of biofilm formed on the DGT surface was analyzed, and the effect of biofouling on the diffusion coefficient of the analyte was investigated. The corrected diffusion coefficient for the biofouled DGT was estimated and used for the calculation of the DGT equation. PIP-binding gels had a higher adsorption affinity for orthophosphate than for the other anions, indicating its selectivity for orthophosphate. The concentrations predicted via DGT agreed well with the concentrations determined in the bulk solutions. Sampling of orthophosphate using PIP-DGT was consistent over a pH range of 3-9 and ionic strength range of 0.01-10 000 μM. Other P compounds cannot be measured using the PIP-DGT technique. The diffusion coefficient of the orthophosphate linearly decreased with increasing thickness of the biofilm. This sampling method performed predictably in freshwater when the biofilm was not formed or when value for the biofilm interference was reduced by using the corrected diffusion coefficient.

  9. On the errors of local density (LDA) and generalized gradient (GGA) approximations to the Kohn-Sham potential and orbital energies.

    Science.gov (United States)

    Gritsenko, O V; Mentel, Ł M; Baerends, E J

    2016-05-28

    In spite of the high quality of exchange-correlation energies Exc obtained with the generalized gradient approximations (GGAs) of density functional theory, their xc potentials vxc are strongly deficient, yielding upshifts of ca. 5 eV in the orbital energy spectrum (in the order of 50% of high-lying valence orbital energies). The GGAs share this deficiency with the local density approximation (LDA). We argue that this error is not caused by the incorrect long-range asymptotics of vxc or by self-interaction error. It arises from incorrect density dependencies of LDA and GGA exchange functionals leading to incorrect (too repulsive) functional derivatives (i.e., response parts of the potentials). The vxc potential is partitioned into the potential of the xc hole vxchole (twice the xc energy density ϵxc), which determines Exc, and the response potential vresp, which does not contribute to Exc explicitly. The substantial upshift of LDA/GGA orbital energies is due to a too repulsive LDA exchange response potential vxresp (LDA) in the bulk region. Retaining the LDA exchange hole potential plus the B88 gradient correction to it but replacing the response parts of these potentials by the model orbital-dependent response potential vxresp (GLLB) of Gritsenko et al. [Phys. Rev. A 51, 1944 (1995)], which has the proper step-wise form, improves the orbital energies by more than an order of magnitude. Examples are given for the prototype molecules: dihydrogen, dinitrogen, carbon monoxide, ethylene, formaldehyde, and formic acid.

  10. High density of tree-cavities and snags in tropical dry forest of western Mexico raises questions for a latitudinal gradient.

    Directory of Open Access Journals (Sweden)

    Leopoldo Vázquez

    Full Text Available It has been suggested that a latitudinal gradient exists of a low density of snags and high density of naturally-formed tree-cavities in tropical vs. temperate forests, though few cavities may have characteristics suitable for nesting by birds. We determined snag and cavity density, characteristics, and suitability for birds in a tropical dry forest biome of western Mexico, and evaluated whether our data fits the trend of snag and cavity density typically found in tropical moist and wet forests. We established five 0.25-ha transects to survey and measure tree-cavities and snags in each of three vegetation types of deciduous, semi-deciduous, and mono-dominant Piranhea mexicana forest, comprising a total of 3.75 ha. We found a high density of 77 cavities/ha, with 37 cavities suitable for birds/ha, where density, and characteristics of cavities varied significantly among vegetation types. Lowest abundance of cavities occurred in deciduous forest, and these were in smaller trees, at a lower height, and with a narrower entrance diameter. Only 8.6% of cavities were excavated by woodpeckers, and only 11% of cavities were occupied, mainly by arthropods, though 52% of all cavities were unsuitable for birds. We also found a high density of 56 snags/ha, with greatest density in deciduous forest (70 snags/ha, though these were of significantly smaller diameter, and snags of larger diameter were more likely to contain cavities. The Chamela-Cuixmala tropical dry forest had the highest density of snags recorded for any tropical or temperate forest, and while snag density was significantly correlated with mean snag dbh, neither latitude nor mean dbh predicted snag density in ten forest sites. The high spatial aggregation of snag and cavity resources in tropical dry forest may limit their availability, particularly for large-bodied cavity adopters, and highlights the importance of habitat heterogeneity in providing resources for primary and secondary cavity-nesters.

  11. High density of tree-cavities and snags in tropical dry forest of western Mexico raises questions for a latitudinal gradient.

    Science.gov (United States)

    Vázquez, Leopoldo; Renton, Katherine

    2015-01-01

    It has been suggested that a latitudinal gradient exists of a low density of snags and high density of naturally-formed tree-cavities in tropical vs. temperate forests, though few cavities may have characteristics suitable for nesting by birds. We determined snag and cavity density, characteristics, and suitability for birds in a tropical dry forest biome of western Mexico, and evaluated whether our data fits the trend of snag and cavity density typically found in tropical moist and wet forests. We established five 0.25-ha transects to survey and measure tree-cavities and snags in each of three vegetation types of deciduous, semi-deciduous, and mono-dominant Piranhea mexicana forest, comprising a total of 3.75 ha. We found a high density of 77 cavities/ha, with 37 cavities suitable for birds/ha, where density, and characteristics of cavities varied significantly among vegetation types. Lowest abundance of cavities occurred in deciduous forest, and these were in smaller trees, at a lower height, and with a narrower entrance diameter. Only 8.6% of cavities were excavated by woodpeckers, and only 11% of cavities were occupied, mainly by arthropods, though 52% of all cavities were unsuitable for birds. We also found a high density of 56 snags/ha, with greatest density in deciduous forest (70 snags/ha), though these were of significantly smaller diameter, and snags of larger diameter were more likely to contain cavities. The Chamela-Cuixmala tropical dry forest had the highest density of snags recorded for any tropical or temperate forest, and while snag density was significantly correlated with mean snag dbh, neither latitude nor mean dbh predicted snag density in ten forest sites. The high spatial aggregation of snag and cavity resources in tropical dry forest may limit their availability, particularly for large-bodied cavity adopters, and highlights the importance of habitat heterogeneity in providing resources for primary and secondary cavity-nesters.

  12. Early and long-term impacts of browsing by roe deer in oak coppiced woods along a gradient of population density

    Directory of Open Access Journals (Sweden)

    Francesco Chianucci

    2015-01-01

    Full Text Available Over the last few decades, wild ungulate populations have exhibited relevant geographic and demographic expansion in most European countries; roe deer is amongst the most widespread ungulate species. The increasing roe deer densities have led to strong impact on forest regeneration; the problem has been recently recognized in coppice woods, a silvicultural system which is widespread in Italy, where it amounts to about 56% of the total national forested area.In this study we investigated the effect of roe deer browsing on the vegetative regeneration of Turkey oak few years after coppicing, along a gradient of roe deer density. A browsing index revealed that browsing impact was high at any given roe deer density but increased at higher density, with the browsing rate ranging from 65% to 79%. We also analyzed the long-term impact of browsing six and eleven years after coppicing under a medium roe deer density. Results indicated the early impact are not ephemeral but produced prolonged impacts through time, with an average reduction in volume of -57% and -41% six and eleven years after coppicing, respectively. Based on these results we proposed integrating browsing monitoring with roe deer density estimation to allow identifying ungulate densities which are compatible with silvicultural and forest management objectives. The proposed browsing index can be regarded as an effective management tool, on account of its simplicity and cost-effectiveness, being therefore highly suitable for routine, large scale monitoring of browsing impact.

  13. Laplacian-Level Kinetic Energy Approximations Based on the Fourth-Order Gradient Expansion: Global Assessment and Application to the Subsystem Formulation of Density Functional Theory.

    Science.gov (United States)

    Laricchia, Savio; Constantin, Lucian A; Fabiano, Eduardo; Della Sala, Fabio

    2014-01-14

    We tested Laplacian-level meta-generalized gradient approximation (meta-GGA) noninteracting kinetic energy functionals based on the fourth-order gradient expansion (GE4). We considered several well-known Laplacian-level meta-GGAs from the literature (bare GE4, modified GE4, and the MGGA functional of Perdew and Constantin (Phys. Rev. B 2007,75, 155109)), as well as two newly designed Laplacian-level kinetic energy functionals (L0.4 and L0.6). First, a general assessment of the different functionals is performed to test them for model systems (one-electron densities, Hooke's atom, and different jellium systems) and atomic and molecular kinetic energies as well as for their behavior with respect to density-scaling transformations. Finally, we assessed, for the first time, the performance of the different functionals for subsystem density functional theory (DFT) calculations on noncovalently interacting systems. We found that the different Laplacian-level meta-GGA kinetic functionals may improve the description of different properties of electronic systems, but no clear overall advantage is found over the best GGA functionals. Concerning the subsystem DFT calculations, the here-proposed L0.4 and L0.6 kinetic energy functionals are competitive with state-of-the-art GGAs, whereas all other Laplacian-level functionals fail badly. The performance of the Laplacian-level functionals is rationalized thanks to a two-dimensional reduced-gradient and reduced-Laplacian decomposition of the nonadditive kinetic energy density.

  14. Evaluation results on the effectiveness of the corrosion protection system for underground pipelines, using the DC-voltage gradient technique

    Energy Technology Data Exchange (ETDEWEB)

    Ribeiro, Antonio Carlos [Petroquimica Uniao S.A., Santo Andre, SP (Brazil)

    2003-07-01

    A pipeline of diameter 6'' with 17.694 km of extension was evaluated how much to the integrity of its external covering and the effectiveness of the system of cathodic protection, using the method DC-Voltage Gradient. The Cathodic Protection in this pipeline is made by seven rectifiers. The gotten data indicate that the potential pipe-ground registered in some check points to the long one of the pipeline is extremely negative, what has led to a super protection of the pipeline for the CP. This if explains for the great proximity between the anodes and the pipeline. For km had been identified 917 failures with a mean density of 50,1 per Km. Beyond the analysis of the data, this work includes conclusions and recommendations detailed for the repairs of the covering of the pipeline and for improvement of the effectiveness of the CP. One sends regards to a combination of repairs in the covering and reevaluation of the CP, in way to improve the protection level and to assure the reduction of the risk of external corrosion. (author)

  15. Density-dependent regulation of brook trout population dynamics along a core-periphery distribution gradient in a central Appalachian watershed.

    Directory of Open Access Journals (Sweden)

    Brock M Huntsman

    Full Text Available Spatial population models predict strong density-dependence and relatively stable population dynamics near the core of a species' distribution with increasing variance and importance of density-independent processes operating towards the population periphery. Using a 10-year data set and an information-theoretic approach, we tested a series of candidate models considering density-dependent and density-independent controls on brook trout population dynamics across a core-periphery distribution gradient within a central Appalachian watershed. We sampled seven sub-populations with study sites ranging in drainage area from 1.3-60 km(2 and long-term average densities ranging from 0.335-0.006 trout/m. Modeled response variables included per capita population growth rate of young-of-the-year, adult, and total brook trout. We also quantified a stock-recruitment relationship for the headwater population and coefficients of variability in mean trout density for all sub-populations over time. Density-dependent regulation was prevalent throughout the study area regardless of stream size. However, density-independent temperature models carried substantial weight and likely reflect the effect of year-to-year variability in water temperature on trout dispersal between cold tributaries and warm main stems. Estimated adult carrying capacities decreased exponentially with increasing stream size from 0.24 trout/m in headwaters to 0.005 trout/m in the main stem. Finally, temporal variance in brook trout population size was lowest in the high-density headwater population, tended to peak in mid-sized streams and declined slightly in the largest streams with the lowest densities. Our results provide support for the hypothesis that local density-dependent processes have a strong control on brook trout dynamics across the entire distribution gradient. However, the mechanisms of regulation likely shift from competition for limited food and space in headwater streams to

  16. Formulation of a Lagrangian stochastic model of dispersion in the convective boundary layer with skewed turbulence conditions and vertical density gradient

    Science.gov (United States)

    cassiani, massimo; stohl, andreas; brioude, jerome

    2014-05-01

    The vertical gradient of air density has been included in a skewed probability density function formulation for turbulence in the convective boundary layer and the related drift term for Lagrangian stochastic particle modelling has been obtained based on the well-mixed condition. The formulation has been extended to include unsteady turbulence statistics. Tests were carried out to validate the model including consistency between forward and backward simulations and preservation of well-mixed state with unsteady conditions. The stationary state CBL drift term with density correction was incorporated in the FLEXPART/FLEXPART-WRF Lagrangian models. Currently only the steady state horizontally homogeneous drift term were included. To avoid numerical instability, using the steady homogenous drift in the presence of non-stationary and horizontally non-homogeneous conditions, a re-initialization procedure for particle velocity was used. The criteria for re-initialization and resulting errors were assessed.

  17. Measurement of deuterium density profiles in the H-mode steep gradient region using charge exchange recombination spectroscopy on DIII-D

    Science.gov (United States)

    Haskey, S. R.; Grierson, B. A.; Burrell, K. H.; Chrystal, C.; Groebner, R. J.; Kaplan, D. H.; Pablant, N. A.; Stagner, L.

    2016-11-01

    Recent completion of a thirty two channel main-ion (deuterium) charge exchange recombination spectroscopy (CER) diagnostic on the DIII-D tokamak [J. L. Luxon, Nucl. Fusion 42, 614 (2002)] enables detailed comparisons between impurity and main-ion temperature, density, and toroidal rotation. In a H-mode DIII-D discharge, these new measurement capabilities are used to provide the deuterium density profile, demonstrate the importance of profile alignment between Thomson scattering and CER diagnostics, and aid in determining the electron temperature at the separatrix. Sixteen sightlines cover the core of the plasma and another sixteen are densely packed towards the plasma edge, providing high resolution measurements across the pedestal and steep gradient region in H-mode plasmas. Extracting useful physical quantities such as deuterium density is challenging due to multiple photoemission processes. These challenges are overcome using a detailed fitting model and by forward modeling the photoemission using the FIDASIM code, which implements a comprehensive collisional radiative model.

  18. Electric field gradients of transition metal complexes from density functional theory: assessment of functionals, geometries and basis sets.

    Science.gov (United States)

    Bjornsson, Ragnar; Bühl, Michael

    2010-06-14

    Electric field gradients (EFGs) were computed for the first-row transition metal nuclei in Cr(C(6)H(6))(CO)(3), MnO(3)F, Mn(CO)(5)H, MnCp(CO)(3), Co(CO)(4)H, Co(CO)(3)(NO) and VCp(CO)(4), for which experimental gas-phase data (in form of nuclear quadrupole coupling constants) are available from microwave spectroscopy. A variety of exchange-correlation functionals were assessed, among which range-separated hybrids (such as CAM-B3LYP or LC-omegaPBE) perform best, followed by global hybrids (such as B3LYP and PBE0) and gradient-corrected functionals (such as BP86). While large basis sets are required on the metal atom for converged EFGs, smaller basis sets can be employed on the ligands. In most cases, EFGs show little sensitivity toward the geometrical parameters.

  19. Schlieren technique applied to the arc temperature measurement in a high energy density cutting torch

    Science.gov (United States)

    Prevosto, L.; Artana, G.; Mancinelli, B.; Kelly, H.

    2010-01-01

    Plasma temperature and radial density profiles of the plasma species in a high energy density cutting arc have been obtained by using a quantitative schlieren technique. A Z-type two-mirror schlieren system was used in this research. Due to its great sensibility such technique allows measuring plasma composition and temperature from the arc axis to the surrounding medium by processing the gray-level contrast values of digital schlieren images recorded at the observation plane for a given position of a transverse knife located at the exit focal plane of the system. The technique has provided a good visualization of the plasma flow emerging from the nozzle and its interactions with the surrounding medium and the anode. The obtained temperature values are in good agreement with those values previously obtained by the authors on the same torch using Langmuir probes.

  20. Laplacian-level kinetic energy approximations based on the fourth-order gradient expansion: Global assessment and application to the subsystem formulation of density functional theory

    CERN Document Server

    Laricchia, S; Fabiano, E; Della Sala, F

    2014-01-01

    We test Laplacian-level meta-generalized gradient approximation (meta-GGA) non-interacting kinetic energy functionals based on the fourth-order gradient expansion (GE4). We consider several well known Laplacian-level meta-GGAs from literature (bare GE4, modified GE4, and the MGGA functional of Perdew and Constantin [Phys. Rev. B \\textbf{75},155109 (2007)]), as well as two newly designed Laplacian-level kinetic energy functionals (named L0.4 and L0.6). First, a general assessment of the different functionals is performed, testing them for model systems (one-electron densities, Hooke's atom and different jellium systems), atomic and molecular kinetic energies as well as for their behavior with respect to density-scaling transformations. Finally, we assess, for the first time, the performance of the different functionals for Subsystem Density Functional Theory (DFT) calculations on non-covalently interacting systems. We find that the different Laplacian-level meta-GGA kinetic functionals may improve the descript...

  1. Nasolacrimal system: evaluation with gadolinium-enhanced MR dacryocystography with a three-dimensional fast spoiled gradient-recalled technique

    Energy Technology Data Exchange (ETDEWEB)

    Karaguelle, Tuba; Erden, Ayse; Erden, Ilhan [Department of Radiology, Medical School, Ankara University, Sihhiye, 06100 Ankara (Turkey); Zilelioglu, Gueler [Department of Ophthalmology, Medical School, Ankara University, Sihhiye, 06100 Ankara (Turkey)

    2002-09-01

    The aim of this study was to determine the diagnostic accuracy of gadolinium-enhanced three-dimensional (3D) fast spoiled gradient-recalled (FSPGR) MR dacryocystography in patients with epiphora. Bilateral nasolacrimal systems of 19 patients suspected of having nasolacrimal canal obstruction were evaluated with MR dacryocystography. A sterile 0.9% NaCl solution containing 1:100 diluted gadolinium chelate was instilled into the bilateral conjunctival sacs of the patients. The 3D FSPGR sequence was used with 1.0-T scanner with the following parameters: TR 9.3 ms; TE 2 ms; flip angle 20; 256 x 224 matrix; 13- or 26-cm field of view; and 1-mm slice thickness. Seventeen patients had digital dacryocystography for comparison which we considered standard of reference. Thirty-four nasolacrimal systems were evaluated with MR and digital dacryocystography. Discrepancies between the findings of both methods were detected in 2 patients. The overall sensitivity of MR in detecting the obstruction was 100%. Magnetic resonance helped to determine the canalicular and ductal obstruction in 100% of the patients and the saccular obstruction in 80% of the patients. We conclude that 3D FSGR technique for MR dacryocystography is a reliable and noninvasive method in the evaluation of the obstruction level in the lacrimal system in patients with epiphora. (orig.)

  2. Recent progress in oxide scintillation crystals development by low-thermal gradient Czochralski technique for particle physics experiments

    Science.gov (United States)

    Shlegel, V. N.; Borovlev, Yu. A.; Grigoriev, D. N.; Grigorieva, V. D.; Danevich, F. A.; Ivannikova, N. V.; Postupaeva, A. G.; Vasiliev, Ya. V.

    2017-08-01

    Modern particle physics experiments call for high performance scintillation detectors with unique properties: radiation-resistant in high energy and astrophysics, highly radiopure, containing certain elements or enriched isotopes in astroparticle physics. The low-thermal gradient Czochralski (LTG CZ) crystal growth technique provides excellent quality large volume radiopure crystal scintillators. Absence of thermoelastic stress in the crystal and overheating of the melt in the LTG CZ method is particularly significant in production of crystalline materials with strong thermal anisotropic properties and low mechanical strength, with a very high yield of crystalline boules and low losses of initial charge, crucially important in production of crystal scintillators from enriched isotopes for double beta decay experiments. Here we discuss progress in development of the well known scintillators (Bi4Ge3O12 (BGO), CdWO4, ZnWO4, CaMoO4, PbMoO4), as well as R&D of new materials (ZnMoO4, Li2MoO4, Na2Mo2O7) for the next generation experiments in particle physics.

  3. Derivation of general analytic gradient expressions for density-fitted post-Hartree-Fock methods: an efficient implementation for the density-fitted second-order Møller-Plesset perturbation theory.

    Science.gov (United States)

    Bozkaya, Uğur

    2014-09-28

    General analytic gradient expressions (with the frozen-core approximation) are presented for density-fitted post-HF methods. An efficient implementation of frozen-core analytic gradients for the second-order Møller-Plesset perturbation theory (MP2) with the density-fitting (DF) approximation (applying to both reference and correlation energies), which is denoted as DF-MP2, is reported. The DF-MP2 method is applied to a set of alkanes, conjugated dienes, and noncovalent interaction complexes to compare the computational cost of single point analytic gradients with MP2 with the resolution of the identity approach (RI-MP2) [F. Weigend and M. Häser, Theor. Chem. Acc. 97, 331 (1997); R. A. Distasio, R. P. Steele, Y. M. Rhee, Y. Shao, and M. Head-Gordon, J. Comput. Chem. 28, 839 (2007)]. In the RI-MP2 method, the DF approach is used only for the correlation energy. Our results demonstrate that the DF-MP2 method substantially accelerate the RI-MP2 method for analytic gradient computations due to the reduced input/output (I/O) time. Because in the DF-MP2 method the DF approach is used for both reference and correlation energies, the storage of 4-index electron repulsion integrals (ERIs) are avoided, 3-index ERI tensors are employed instead. Further, as in case of integrals, our gradient equation is completely avoid construction or storage of the 4-index two-particle density matrix (TPDM), instead we use 2- and 3-index TPDMs. Hence, the I/O bottleneck of a gradient computation is significantly overcome. Therefore, the cost of the generalized-Fock matrix (GFM), TPDM, solution of Z-vector equations, the back transformation of TPDM, and integral derivatives are substantially reduced when the DF approach is used for the entire energy expression. Further application results show that the DF approach introduce negligible errors for closed-shell reaction energies and equilibrium bond lengths.

  4. Derivation of general analytic gradient expressions for density-fitted post-Hartree-Fock methods: An efficient implementation for the density-fitted second-order Møller–Plesset perturbation theory

    Energy Technology Data Exchange (ETDEWEB)

    Bozkaya, Uğur, E-mail: ugur.bozkaya@atauni.edu.tr [Department of Chemistry, Atatürk University, Erzurum 25240, Turkey and Department of Chemistry, Middle East Technical University, Ankara 06800 (Turkey)

    2014-09-28

    General analytic gradient expressions (with the frozen-core approximation) are presented for density-fitted post-HF methods. An efficient implementation of frozen-core analytic gradients for the second-order Møller–Plesset perturbation theory (MP2) with the density-fitting (DF) approximation (applying to both reference and correlation energies), which is denoted as DF-MP2, is reported. The DF-MP2 method is applied to a set of alkanes, conjugated dienes, and noncovalent interaction complexes to compare the computational cost of single point analytic gradients with MP2 with the resolution of the identity approach (RI-MP2) [F. Weigend and M. Häser, Theor. Chem. Acc. 97, 331 (1997); R. A. Distasio, R. P. Steele, Y. M. Rhee, Y. Shao, and M. Head-Gordon, J. Comput. Chem. 28, 839 (2007)]. In the RI-MP2 method, the DF approach is used only for the correlation energy. Our results demonstrate that the DF-MP2 method substantially accelerate the RI-MP2 method for analytic gradient computations due to the reduced input/output (I/O) time. Because in the DF-MP2 method the DF approach is used for both reference and correlation energies, the storage of 4-index electron repulsion integrals (ERIs) are avoided, 3-index ERI tensors are employed instead. Further, as in case of integrals, our gradient equation is completely avoid construction or storage of the 4-index two-particle density matrix (TPDM), instead we use 2- and 3-index TPDMs. Hence, the I/O bottleneck of a gradient computation is significantly overcome. Therefore, the cost of the generalized-Fock matrix (GFM), TPDM, solution of Z-vector equations, the back transformation of TPDM, and integral derivatives are substantially reduced when the DF approach is used for the entire energy expression. Further application results show that the DF approach introduce negligible errors for closed-shell reaction energies and equilibrium bond lengths.

  5. Comparison of Conjugate Gradient Density Matrix Search and Chebyshev Expansion Methods for Avoiding Diagonalization in Large-Scale Electronic Structure Calculations

    Science.gov (United States)

    Bates, Kevin R.; Daniels, Andrew D.; Scuseria, Gustavo E.

    1998-01-01

    We report a comparison of two linear-scaling methods which avoid the diagonalization bottleneck of traditional electronic structure algorithms. The Chebyshev expansion method (CEM) is implemented for carbon tight-binding calculations of large systems and its memory and timing requirements compared to those of our previously implemented conjugate gradient density matrix search (CG-DMS). Benchmark calculations are carried out on icosahedral fullerenes from C60 to C8640 and the linear scaling memory and CPU requirements of the CEM demonstrated. We show that the CPU requisites of the CEM and CG-DMS are similar for calculations with comparable accuracy.

  6. Desvio da proporção de sexo e da integridade do DNA dos espermatozóides bovinos centrifugados em gradientes de densidade contínuos Alteration of sex ratio and DNA integrity of bovine sperm centrifuged in continuous density gradients

    Directory of Open Access Journals (Sweden)

    Alberto Lopes Gusmão

    2010-03-01

    Full Text Available O objetivo, neste trabalho, foi verificar o desvio da proporção de sexo e a presença de fragmentação do DNA, pela técnica de TUNEL (“In situ terminal deoxinucleotidyl transferase mediated dUTP nick end labeling assay”, em espermatozoides bovinos centrifugados em gradientes de densidade de Percoll ou OptiPrep durante a separação espermática. Doses de sêmen de touros foram descongeladas, e cerca de 40 milhões de espermatozoides foram depositados sobre cada gradiente de densidade compostos por Percoll ou OptiPrep com três camadas entre 1.110g/mL e 1.123g/mL, em tubos de 15mL, em que permaneceram por 24h a 4°C antes da deposição dos espermatozoides. Os tubos foram centrifugados a 500xg por 15min a 22°C. Os sobrenadantes foram aspirados, e os sedimentos, recuperados para verificação da fragmentação do DNA pela técnica de TUNEL. Obteve-se um desvio dos embriões produzidos in vitro para fêmeas no gradiente de Percoll (62% de fêmeas, em relação aos grupos OptiPrep e Controle (47,1 e 48,7% de fêmeas, respectivamente. Não foi detectada fragmentação do DNA dos espermatozoides nas amostras centrifugadas, tanto no gradiente de Percoll quanto de OptiPrep. Dessa forma, foi possível realizar a sexagem espermática, com uma maior porcentagem de espermatozoides X do que o grupo controle, por meio de metodologia mais simples e sem provocar danos ao DNA dos espermatozoides.The objective of the present study was to verify the sex ratio and presence of DNA fragmentation by TUNEL technique (In situ terminal deoxinucleotidyl transferase mediated dUTP nick end labeling assay in bovine spermatozoa centrifuged in density gradients of Percoll or OptiPrep during the sperm separation. Approximately 40 million of frozen/thawed bovine spermatozoa were deposited on each density gradient composed of Percoll or OptiPrep with three layers ranging from 1.110g/mL to 1.123g/mL in polystyrene tubes of 15mL. The tubes were kept at 4°C for 24h before

  7. Relativistically induced transparency acceleration of light ions by an ultrashort laser pulse interacting with a heavy-ion-plasma density gradient

    Science.gov (United States)

    Sahai, Aakash A.; Tsung, Frank S.; Tableman, Adam R.; Mori, Warren B.; Katsouleas, Thomas C.

    2013-10-01

    The relativistically induced transparency acceleration (RITA) scheme of proton and ion acceleration using laser-plasma interactions is introduced, modeled, and compared to the existing schemes. Protons are accelerated with femtosecond relativistic pulses to produce quasimonoenergetic bunches with controllable peak energy. The RITA scheme works by a relativistic laser inducing transparency [Akhiezer and Polovin, Zh. Eksp. Teor. Fiz 30, 915 (1956); Kaw and Dawson, Phys. FluidsPFLDAS0031-917110.1063/1.1692942 13, 472 (1970); Max and Perkins, Phys. Rev. Lett.PRLTAO0031-900710.1103/PhysRevLett.27.1342 27, 1342 (1971)] to densities higher than the cold-electron critical density, while the background heavy ions are stationary. The rising laser pulse creates a traveling acceleration structure at the relativistic critical density by ponderomotively [Lindl and Kaw, Phys. FluidsPFLDAS0031-917110.1063/1.1693437 14, 371 (1971); Silva , Phys. Rev. E1063-651X10.1103/PhysRevE.59.2273 59, 2273 (1999)] driving a local electron density inflation, creating an electron snowplow and a co-propagating electrostatic potential. The snowplow advances with a velocity determined by the rate of the rise of the laser's intensity envelope and the heavy-ion-plasma density gradient scale length. The rising laser is incrementally rendered transparent to higher densities such that the relativistic-electron plasma frequency is resonant with the laser frequency. In the snowplow frame, trace density protons reflect off the electrostatic potential and get snowplowed, while the heavier background ions are relatively unperturbed. Quasimonoenergetic bunches of velocity equal to twice the snowplow velocity can be obtained and tuned by controlling the snowplow velocity using laser-plasma parameters. An analytical model for the proton energy as a function of laser intensity, rise time, and plasma density gradient is developed and compared to 1D and 2D PIC OSIRIS [Fonseca , Lect. Note Comput. Sci.9783

  8. Digital processing techniques and film density calibration for printing image data

    Science.gov (United States)

    Chavez, Pat S.; McSweeney, Joseph A.; Binnie, Douglas R.

    1987-01-01

    Satellite image data that cover a wide range of environments are being used to make prints that represent a map type product. If a wide distribution of these products is desired, they are printed using lithographic rather than photographic procedures to reduce the cost per print. Problems are encountered in the photo lab if the film products to be used for lithographic printing have the same density range and density curve characteristics as the film used for photographic printing. A method is presented that keeps the film densities within the 1.1 range required for lithographic printing, but generates film products with contrast similar to that in photographic film for the majority of data (80 percent). Also, spatial filters can be used to enhance local detail in dark and bright regions, as well as to sharpen the final image product using edge enhancement techniques.

  9. Assessment of density functional theory optimized basis sets for gradient corrected functionals to transition metal systems: the case of small Nin (n

    Science.gov (United States)

    López Arvizu, Gregorio; Calaminici, Patrizia

    2007-05-21

    Density functional calculations have been performed for small nickel clusters, Ni(n), Ni(n) (+), and Ni(n)(-) (ntheory approach. Newly developed nickel all-electron basis sets optimized for generalized gradient approximation (GGA) as well as an all-electron basis set optimized for the local density approximation were employed. For both neutral and charged systems, several isomers and different multiplicities were studied in order to determine the lowest energy structures. A vibrational analysis was performed in order to characterize these isomers. Structural parameters, harmonic frequencies, binding energies, ionization potentials, and electron affinities are reported. This work shows that the employed GGA basis sets for the nickel atom are important for the correct prediction of the ground state structures of small nickel clusters and that the structural assignment of these systems can be performed, with a good resolution, over the ionization potential.

  10. Memory,Time and Technique Aspects of Density Matrix Renormalization Group Method

    Institute of Scientific and Technical Information of China (English)

    QIN Shao-Jin; LOU Ji-Zhong

    2001-01-01

    We present the memory size,computational time,and technique aspects of density matrix renormalization group (DMRG) algorithm.We show how to estimate the memory size and computational time before starting a large scale DMRG calculation.We propose an implementation of the Hamiltonian wavefunction multiplication and a wavefunction initialization in DMRG with block matrix data structure.One-dimensional Heisenberg model is used to illustrate our study.``

  11. Error Analysis of a Fractional Time-Stepping Technique for Incompressible Flows with Variable Density

    KAUST Repository

    Guermond, J.-L.

    2011-01-01

    In this paper we analyze the convergence properties of a new fractional time-stepping technique for the solution of the variable density incompressible Navier-Stokes equations. The main feature of this method is that, contrary to other existing algorithms, the pressure is determined by just solving one Poisson equation per time step. First-order error estimates are proved, and stability of a formally second-order variant of the method is established. © 2011 Society for Industrial and Applied Mathematics.

  12. 3D-Laser-Scanning Technique Applied to Bulk Density Measurements of Apollo Lunar Samples

    Science.gov (United States)

    Macke, R. J.; Kent, J. J.; Kiefer, W. S.; Britt, D. T.

    2015-01-01

    In order to better interpret gravimetric data from orbiters such as GRAIL and LRO to understand the subsurface composition and structure of the lunar crust, it is import to have a reliable database of the density and porosity of lunar materials. To this end, we have been surveying these physical properties in both lunar meteorites and Apollo lunar samples. To measure porosity, both grain density and bulk density are required. For bulk density, our group has historically utilized sub-mm bead immersion techniques extensively, though several factors have made this technique problematic for our work with Apollo samples. Samples allocated for measurement are often smaller than optimal for the technique, leading to large error bars. Also, for some samples we were required to use pure alumina beads instead of our usual glass beads. The alumina beads were subject to undesirable static effects, producing unreliable results. Other investigators have tested the use of 3d laser scanners on meteorites for measuring bulk volumes. Early work, though promising, was plagued with difficulties including poor response on dark or reflective surfaces, difficulty reproducing sharp edges, and large processing time for producing shape models. Due to progress in technology, however, laser scanners have improved considerably in recent years. We tested this technique on 27 lunar samples in the Apollo collection using a scanner at NASA Johnson Space Center. We found it to be reliable and more precise than beads, with the added benefit that it involves no direct contact with the sample, enabling the study of particularly friable samples for which bead immersion is not possible

  13. 密度梯度碳/碳复合材料的制备及性能%Preparation and Properties of Density Gradient Carbon/Carbon Composites

    Institute of Scientific and Technical Information of China (English)

    陈强; 张守阳

    2011-01-01

    采用强制流动热梯度化学气相渗透法在1000~1250℃制备了密度梯度碳/碳复合材料;借助三点弯曲试验和激光闪烁法测定了复合材料的弯曲性能与导热系数,用偏光显微镜及扫描电子显微镜观察了基体热解碳的组织结构及断口形貌。结果表明:该复合材料上层的最大密度为1.65g·cm^-3,下层的最小密度为1.10g·cm^-3,具有明显的密度梯度;复合材料的密度越大,抗弯强度越高;其导热系数也随密度的增加而增大;沉积温度是影响基体热解碳组织的主要因素,高温有利于粗糙层热解碳的生成,而低温有利于光滑层热解碳的生成。%Density gradient carbon/carbon composites were infiltrated using forced flow thermal gradient chemical vapor infiltration in the range of 1 000- 1 250℃. Flexural strength and thermal conductivity were determined by 3 point bending test and laser flashing method. Mierostructure of deposited pyrolytic carbon and morphology of fracture surface were observed by polarized light microscopy and scanning electron microscopy. The results show that maximal density of upper part of the composites was 1.65 g · cm^-3 , while miniumum density of bottom part was 1. 10 g ·em^-3 , and obvions density gradient was found. Flexural strength and coefficient of thermal conductivity increased with the increase of density of the composites. The deposition temperature had a great impact on the mierostrueture of the pyolytic carbon. A higher temperature was favourable for the formation of rough layer hydrocarbon, while a lower temperature was favourable for the formation of smooth layer hydrocarbon.

  14. Predicting the morphological characteristics and basic density of Eucalyptus wood using the NIRS technique

    Directory of Open Access Journals (Sweden)

    Lívia Cássia Viana

    2009-12-01

    Full Text Available This work aimed to apply the near infrared spectroscopy technique (NIRS for fast prediction of basic density and morphological characteristics of wood fibers in Eucalyptus clones. Six Eucalyptus clones aged three years were used, obtained from plantations in Cocais, Guanhães, Rio Doce and Santa Bárbara, in Minas Gerais state. The morphological characteristics of the fibers and basic density of the wood were determined by conventional methods and correlated with near infrared spectra using partial least square regression (PLS regression. Best calibration correlations were obtained in basic density prediction, with values 0.95 for correlation coefficient of cross validation (Rcv and 3.4 for ratio performance deviation (RPD, in clone 57. Fiber length can be predicted by models with Rcv ranging from 0.61 to 0.89 and standard error (SECV ranging from 0.037 to 0.079 mm. The prediction model for wood fiber width presented higher Rcv (0.82 and RPD (1.9 values in clone 1046. Best fits to estimate lumen diameter and fiber wall thickness were obtained with information from clone 1046. In some clones, the NIRS technique proved efficient to predict the anatomical properties and basic density of wood in Eucalyptus clones.

  15. Practical technique to quantify small, dense low-density lipoprotein cholesterol using dynamic light scattering

    Science.gov (United States)

    Trirongjitmoah, Suchin; Iinaga, Kazuya; Sakurai, Toshihiro; Chiba, Hitoshi; Sriyudthsak, Mana; Shimizu, Koichi

    2016-04-01

    Quantification of small, dense low-density lipoprotein (sdLDL) cholesterol is clinically significant. We propose a practical technique to estimate the amount of sdLDL cholesterol using dynamic light scattering (DLS). An analytical solution in a closed form has newly been obtained to estimate the weight fraction of one species of scatterers in the DLS measurement of two species of scatterers. Using this solution, we can quantify the sdLDL cholesterol amount from the amounts of the low-density lipoprotein cholesterol and the high-density lipoprotein (HDL) cholesterol, which are commonly obtained through clinical tests. The accuracy of the proposed technique was confirmed experimentally using latex spheres with known size distributions. The applicability of the proposed technique was examined using samples of human blood serum. The possibility of estimating the sdLDL amount using the HDL data was demonstrated. These results suggest that the quantitative estimation of sdLDL amounts using DLS is feasible for point-of-care testing in clinical practice.

  16. A Noncontact Measurement Technique for the Density and Thermal Expansion Coefficient of Solid and Liquid Materials

    Science.gov (United States)

    Chung, Sang K.; Thiessen, David B.; Rhim, Won-Kyu

    1996-01-01

    A noncontact measurement technique for the density and the thermal expansion refractory materials in their molten as well as solid phases is presented. This technique is based on the video image processing of a levitated sample. Experiments were performed using the high-temperature electrostatic levitator (HTESL) at the Jet Propulsion Laboratory in which 2-3 mm diameter samples can be levitated, melted, and radiatively cooled in a vacuum. Due to the axisymmetric nature of the molten samples when levitated in the HTESL, a rather simple digital image analysis can be employed to accurately measure the volumetric change as a function of temperature. Density and the thermal expansion coefficient measurements were made on a pure nickel sample to test the accuracy of the technique in the temperature range of 1045-1565 C. The result for the liquid phase density can be expressed by p = 8.848 + (6.730 x 10(exp -4)) x T (degC) g/cu cm within 0.8% accuracy, and the corresponding thermal expansion coefficient can be expressed by Beta=(9.419 x 10(exp -5)) - (7.165 x 10(exp -9) x T (degC)/K within 0.2% accuracy.

  17. Lagrangian Stochastic Modelling of Dispersion in the Convective Boundary Layer with Skewed Turbulence Conditions and a Vertical Density Gradient: Formulation and Implementation in the FLEXPART Model

    Science.gov (United States)

    Cassiani, Massimo; Stohl, Andreas; Brioude, Jerome

    2015-03-01

    A correction for the vertical gradient of air density has been incorporated into a skewed probability density function formulation for turbulence in the convective boundary layer. The related drift term for Lagrangian stochastic dispersion modelling has been derived based on the well-mixed condition. Furthermore, the formulation has been extended to include unsteady turbulence statistics and the related additional component of the drift term obtained. These formulations are an extension of the drift formulation reported by Luhar et al. (Atmos Environ 30:1407-1418, 1996) following the well-mixed condition proposed by Thomson (J Fluid Mech 180:529-556, 1987). Comprehensive tests were carried out to validate the formulations including consistency between forward and backward simulations and preservation of a well-mixed state with unsteady conditions. The stationary state CBL drift term with density correction was incorporated into the FLEXPART and FLEXPART-WRF Lagrangian models, and included the use of an ad hoc transition function that modulates the third moment of the vertical velocity based on stability parameters. Due to the current implementation of the FLEXPART models, only a steady-state horizontally homogeneous drift term could be included. To avoid numerical instability, in the presence of non-stationary and horizontally inhomogeneous conditions, a re-initialization procedure for particle velocity was used. The criteria for re-initialization and resulting errors were assessed for the case of non-stationary conditions by comparing a reference numerical solution in simplified unsteady conditions, obtained using the non-stationary drift term, and a solution based on the steady drift with re-initialization. Two examples of "real-world" numerical simulations were performed under different convective conditions to demonstrate the effect of the vertical gradient in density on the particle dispersion in the CBL.

  18. On the errors of local density (LDA) and generalized gradient (GGA) approximations to the Kohn-Sham potential and orbital energies

    Science.gov (United States)

    Gritsenko, O. V.; Mentel, Ł. M.; Baerends, E. J.

    2016-05-01

    In spite of the high quality of exchange-correlation energies Exc obtained with the generalized gradient approximations (GGAs) of density functional theory, their xc potentials vxc are strongly deficient, yielding upshifts of ca. 5 eV in the orbital energy spectrum (in the order of 50% of high-lying valence orbital energies). The GGAs share this deficiency with the local density approximation (LDA). We argue that this error is not caused by the incorrect long-range asymptotics of vxc or by self-interaction error. It arises from incorrect density dependencies of LDA and GGA exchange functionals leading to incorrect (too repulsive) functional derivatives (i.e., response parts of the potentials). The vxc potential is partitioned into the potential of the xc hole vxchole (twice the xc energy density ɛxc), which determines Exc, and the response potential vresp, which does not contribute to Exc explicitly. The substantial upshift of LDA/GGA orbital energies is due to a too repulsive LDA exchange response potential vxresp L D A in the bulk region. Retaining the LDA exchange hole potential plus the B88 gradient correction to it but replacing the response parts of these potentials by the model orbital-dependent response potential vxresp G L L B of Gritsenko et al. [Phys. Rev. A 51, 1944 (1995)], which has the proper step-wise form, improves the orbital energies by more than an order of magnitude. Examples are given for the prototype molecules: dihydrogen, dinitrogen, carbon monoxide, ethylene, formaldehyde, and formic acid.

  19. Comparative validation using quantitative real-time PCR (qPCR and conventional PCR of bovine semen centrifuged in continuous density gradient

    Directory of Open Access Journals (Sweden)

    M.V. Resende

    2011-06-01

    Full Text Available The objective of the present study was to determine the sperm enrichment with X-bearing spermatozoa, after one centrifugation in a Percoll or OptiPrep continuous density gradient, using quantitative real-time polymerase chain reaction (qPCR of sperm DNA and resultant in vitro-produced bovine embryos by PCR. Frozen/thawed sperm was layered on density gradients and the tubes were centrifuged. Supernatants were gently aspirated and the sperm recovered from the bottom of the tubes. Cleavage and blastocyst rates were determined through in vitro production of embryos and PCR was performed to identify the embryos' genetic sex. A difference in blastocyst rate was found in the Percoll treatment compared to OptiPrep (P<0.05. The percentage of female embryos in the Percoll and OptiPrep groups was 62.0% and 47.1%, respectively. These results were confirmed by qPCR of spermatozoa DNA and underestimation was seen only in the Percoll group. It was possible to sexing sperm using simple approach.

  20. Electron cyclotron maser emission mode coupling to the z-mode on a longitudinal density gradient in the context of solar type III bursts

    CERN Document Server

    Pechhacker, Roman

    2012-01-01

    A beam of super-thermal, hot electrons was injected into maxwellian plasma with a density gradient along a magnetic field line. 1.5D particle-in-cell simulations were carried out which established that the EM emission is produced by the perpendicular component of the beam injection momentum. The beam has a positive slope in the distribution function in perpendicular momentum phase space, which is the characteristic feature of a cyclotron maser. The cyclotron maser in the overdense plasma generates emission at the electron cyclotron frequency. The frequencies of generated waves were too low to propagate away from the injection region, hence the wavelet transform shows a pulsating wave generation and decay process. The intensity pulsation frequency is twice the relativistic cyclotron frequency. Eventually, a stable wave packet formed and could mode couple on the density gradient to reach frequencies of the order of the plasma frequency, that allowed for propagation. The emitted wave is likely to be a z-mode wav...

  1. Density-Gradient Mediated Band Extraction of Leukocytes from Whole Blood Using Centrifugo-Pneumatic Siphon Valving on Centrifugal Microfluidic Discs.

    Directory of Open Access Journals (Sweden)

    David J Kinahan

    Full Text Available Here we present retrieval of Peripheral Blood Mononuclear Cells by density-gradient medium based centrifugation for subsequent analysis of the leukocytes on an integrated microfluidic "Lab-on-a-Disc" cartridge. Isolation of white blood cells constitutes a critical sample preparation step for many bioassays. Centrifugo-pneumatic siphon valves are particularly suited for blood processing as they function without need of surface treatment and are 'low-pass', i.e., holding at high centrifugation speeds and opening upon reduction of the spin rate. Both 'hydrostatically' and 'hydrodynamically' triggered centrifugo-pneumatic siphon valving schemes are presented. Firstly, the geometry of the pneumatic chamber of hydrostatically primed centrifugo-pneumatic siphon valves is optimised to enable smooth and uniform layering of blood on top of the density-gradient medium; this feature proves to be key for efficient Peripheral Blood Mononuclear Cell extraction. A theoretical analysis of hydrostatically primed valves is also presented which determines the optimum priming pressure for the individual valves. Next, 'dual siphon' configurations for both hydrostatically and hydrodynamically primed centrifugo-pneumatic siphon valves are introduced; here plasma and Peripheral Blood Mononuclear Cells are extracted through a distinct siphon valve. This work represents a first step towards enabling on disc multi-parameter analysis. Finally, the efficiency of Peripheral Blood Mononuclear Cells extraction in these structures is characterised using a simplified design. A microfluidic mechanism, which we termed phase switching, is identified which affects the efficiency of Peripheral Blood Mononuclear Cell extraction.

  2. Thermodynamic study of β-cyclodextrin-dye inclusion complexes using gradient flow injection technique and molecular modeling

    Science.gov (United States)

    Izadmanesh, Y.; Ghasemi, Jahan B.

    2016-08-01

    Gradient flow injection technique-diode array spectrophotometry was applied for β-cyclodextrin (β-CD)-dye inclusion complex studies. A single injection of a small amount of mixed β-CD-dye solution (100 μl) into the carrier solution of the dye and recording the spectra gave the titration data. The mole ratio data were calculated by calibrating the dispersion pattern using a calibrator dye (rose bengal). Model-based multivariate methods were used to analyze the spectral-mole ratio data and, as a result, estimate stability constants and concentration-spectral profiles. Reliability was tested by applying this method to study the β-CD host-guest complexes with several dyes as guest molecules. Singular value decomposition (SVD) was used to select the chemical model and reduce noise. Molecular modeling provided the ability to predict the guest conformation-orientation (posing) within the cavity of β-CD and the nature of the involved interactions. Among those dyes showing observable spectral variation, the stoichiometric ratio of β-CD: dye (and log Kf) of methyl orange, fluorescein, phenol red, 4-(2-pyridylazo) resorcinol (PAR), and crystal violet were calculated to be 1:1 (4.26 ± 0.01), 1:1 (1.53 ± 0.08), 1:1 (3.11 ± 0.04), 1:1 (1.06 ± 0.12), and 2:1 (5.27 ± 0.03), respectively. Compared with the classical method of titration, this method is simple and fast and has the advantage of needing reduced human interference. Molecular modeling facilitates a better understanding of the type of interactions and conformation of guest molecules in the β-CD cavity. The details of the proposed method are discussed in this paper.

  3. Thermodynamic study of β-cyclodextrin-dye inclusion complexes using gradient flow injection technique and molecular modeling.

    Science.gov (United States)

    Izadmanesh, Y; Ghasemi, Jahan B

    2016-08-05

    Gradient flow injection technique-diode array spectrophotometry was applied for β-cyclodextrin (β-CD)-dye inclusion complex studies. A single injection of a small amount of mixed β-CD-dye solution (100μl) into the carrier solution of the dye and recording the spectra gave the titration data. The mole ratio data were calculated by calibrating the dispersion pattern using a calibrator dye (rose bengal). Model-based multivariate methods were used to analyze the spectral-mole ratio data and, as a result, estimate stability constants and concentration-spectral profiles. Reliability was tested by applying this method to study the β-CD host-guest complexes with several dyes as guest molecules. Singular value decomposition (SVD) was used to select the chemical model and reduce noise. Molecular modeling provided the ability to predict the guest conformation-orientation (posing) within the cavity of β-CD and the nature of the involved interactions. Among those dyes showing observable spectral variation, the stoichiometric ratio of β-CD: dye (and log Kf) of methyl orange, fluorescein, phenol red, 4-(2-pyridylazo) resorcinol (PAR), and crystal violet were calculated to be 1:1 (4.26±0.01), 1:1 (1.53±0.08), 1:1 (3.11±0.04), 1:1 (1.06±0.12), and 2:1 (5.27±0.03), respectively. Compared with the classical method of titration, this method is simple and fast and has the advantage of needing reduced human interference. Molecular modeling facilitates a better understanding of the type of interactions and conformation of guest molecules in the β-CD cavity. The details of the proposed method are discussed in this paper.

  4. How Do Vegetation Density and Transportation Network Density Affect Crime across an Urban Central-Peripheral Gradient? A Case Study in Kitchener—Waterloo, Ontario

    Directory of Open Access Journals (Sweden)

    Yikang Du

    2016-07-01

    Full Text Available The relationship between vegetation, transportation networks, and crime has been under debate. Vegetation has been positively correlated with fear of crime; however, the actual correlation between vegetation and occurrences of crime is uncertain. Transportation networks have also been connected with crime occurrence but their impact on crime tends to vary over different circumstances. By conducting spatial analyses, this study explores the associations between crime and vegetation as well as transportation networks in Kitchener-Waterloo. Further, geographically weighted regression modeling and a dummy urban variable representing the urban center/other urban areas were employed to explore the associations across an urban central-peripheral gradient. Associations were analyzed for crimes against persons and crimes against property for four specific crime types (assaults, vehicle theft, sex offences, and drugs. Results suggest that vegetation has a reverse association with crimes against persons and crimes against property while transportation networks have a positive relationship with these two types of crime. Additionally, vegetation can be a deterrent to vehicle theft crime and drugs, while transportation networks can be a facilitator of drug-related crimes. Besides, these two associations appear stronger in the urban center compared to the urban periphery.

  5. Intensity Modulated Proton Therapy for Craniospinal Irradiation: Organ-at-Risk Exposure and a Low-Gradient Junctioning Technique

    Energy Technology Data Exchange (ETDEWEB)

    Stoker, Joshua B., E-mail: stoker.joshua@mayo.edu [Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Department of Radiation Oncology, Mayo Clinic Arizona, Phoenix, Arizona (United States); Grant, Jonathan [Division of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Zhu, X. Ronald; Pidikiti, Rajesh [Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Mahajan, Anita; Grosshans, David R. [Division of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States)

    2014-11-01

    Purpose: To compare field junction robustness and sparing of organs at risk (OARs) during craniospinal irradiation (CSI) using intensity modulated proton therapy (IMPT) to conventional passively scattered proton therapy (PSPT). Methods and Materials: Ten patients, 5 adult and 5 pediatric patients, previously treated with PSPT-based CSI were selected for comparison. Anterior oblique cranial fields, using a superior couch rotation, and posterior spinal fields were used for IMPT planning. To facilitate low-gradient field junctioning along the spine, the inverse-planning IMPT technique was divided into 3 stages. Dose indices describing target coverage and normal tissue dose, in silico error modeling, and film dosimetry were used to assess plan quality. Results: Field junction robustness along the spine was improved using the staged IMPT planning technique, reducing the worst case impact of a 4-mm setup error from 25% in PSPT to <5% of prescription dose. This was verified by film dosimetry for clinical delivery. Exclusive of thyroid dose in adult patients, IMPT plans demonstrated sparing of organs at risk as good or better than PSPT. Coverage of the cribriform plate for pediatric (V95% [percentage of volume of the target receiving at least 95% of the prescribed dose]; 87 ± 11 vs 92 ± 7) and adult (V95%; 94 ± 7 vs 100 ± 1) patients and the clinical target in pediatric (V95%; 98 ± 2 vs 100 ± 1) and adult (V95%; 100 ± 1 vs 100 ± 1) patients for PSPT and IMPT plans, respectively, were comparable or improved. For adult patients, IMPT target dose inhomogeneity was increased, as determined by heterogeneity index (HI) and inhomogeneity coefficient (IC). IMPT lowered maximum spinal cord dose, improved spinal dose homogeneity, and reduced exposure to other OARs. Conclusions: IMPT has the potential to improve CSI plan quality and the homogeneity of intrafractional dose at match lines. The IMPT approach developed may also simplify treatments and reduce

  6. Seismic Hazard Analysis Using the Adaptive Kernel Density Estimation Technique for Chennai City

    Science.gov (United States)

    Ramanna, C. K.; Dodagoudar, G. R.

    2012-01-01

    Conventional method of probabilistic seismic hazard analysis (PSHA) using the Cornell-McGuire approach requires identification of homogeneous source zones as the first step. This criterion brings along many issues and, hence, several alternative methods to hazard estimation have come up in the last few years. Methods such as zoneless or zone-free methods, modelling of earth's crust using numerical methods with finite element analysis, have been proposed. Delineating a homogeneous source zone in regions of distributed seismicity and/or diffused seismicity is rather a difficult task. In this study, the zone-free method using the adaptive kernel technique to hazard estimation is explored for regions having distributed and diffused seismicity. Chennai city is in such a region with low to moderate seismicity so it has been used as a case study. The adaptive kernel technique is statistically superior to the fixed kernel technique primarily because the bandwidth of the kernel is varied spatially depending on the clustering or sparseness of the epicentres. Although the fixed kernel technique has proven to work well in general density estimation cases, it fails to perform in the case of multimodal and long tail distributions. In such situations, the adaptive kernel technique serves the purpose and is more relevant in earthquake engineering as the activity rate probability density surface is multimodal in nature. The peak ground acceleration (PGA) obtained from all the three approaches (i.e., the Cornell-McGuire approach, fixed kernel and adaptive kernel techniques) for 10% probability of exceedance in 50 years is around 0.087 g. The uniform hazard spectra (UHS) are also provided for different structural periods.

  7. Correlations Between the Gradient of Contrast Density, Evaluated by Cardio CT, and Functional Significance of Coronary Artery Stenosis

    Directory of Open Access Journals (Sweden)

    Orzan Marius

    2016-06-01

    Full Text Available Background: Assessment of the hemodynamic significance of a coronary artery stenosis is a challenging task, being extremely important for the establishment of indication for revascularization in atherosclerotic coronary artery stenosis. The aim of this study was to evaluate the role of a new marker reflecting the functional significance of a coronary artery stenosis, represented by the attenuation degree of contrast density along the stenosis by Coronary CT.

  8. Feasibility of measuring density and temperature of laser produced plasmas using spectroscopic techniques.

    Energy Technology Data Exchange (ETDEWEB)

    Edens, Aaron D.

    2008-09-01

    A wide variety of experiments on the Z-Beamlet laser involve the creation of laser produced plasmas. Having a direct measurement of the density and temperature of these plasma would an extremely useful tool, as understanding how these quantities evolve in space and time gives insight into the causes of changes in other physical processes, such as x-ray generation and opacity. We propose to investigate the possibility of diagnosing the density and temperature of laser-produced plasma using temporally and spatially resolved spectroscopic techniques that are similar to ones that have been successfully fielded on other systems. Various researchers have measured the density and temperature of laboratory plasmas by looking at the width and intensity ratio of various characteristic lines in gases such as nitrogen and hydrogen, as well as in plasmas produced off of solid targets such as zinc. The plasma conditions produce two major measurable effects on the characteristic spectral lines of that plasma. The 1st is the Stark broadening of an individual line, which depends on the electron density of the plasma, with higher densities leading to broader lines. The second effect is a change in the ratio of various lines in the plasma corresponding to different ionization states. By looking at the ratio of these lines, we can gain some understanding of the plasma ionization state and consequently its temperature (and ion density when coupled with the broadening measurement). The hotter a plasma is, the higher greater the intensity of lines corresponding to higher ionization states. We would like to investigate fielding a system on the Z-Beamlet laser chamber to spectroscopically study laser produced plasmas from different material targets.

  9. Evaluation of the flux gradient technique for measurement of ozone surface fluxes over snowpack at Summit, Greenland

    Directory of Open Access Journals (Sweden)

    F. Bocquet

    2011-10-01

    Full Text Available A multi-step procedure for investigating ozone surface fluxes over polar snow by the tower gradient method was developed and evaluated. These measurements were then used to obtain five months (April–August 2004 of turbulent ozone flux data at the Summit research camp located in the center of the Greenland ice shield. Turbulent fluxes were determined by the gradient method incorporating tower measurements of (a ozone gradients measured by commercial ultraviolet absorption analyzers, (b ambient temperature gradients using aspirated thermocouple sensors, and (c wind speed gradients determined by cup anemometers. All gradient instruments were regularly inter-compared by bringing sensors or inlets to the same measurement height. The developed protocol resulted in an uncertainty on the order of 0.1 ppbv for 30-min averaged ozone gradients that were used for the ozone flux calculations. This protocol facilitated a lower sensitivity threshold for the ozone flux determination of ∼8 × 10−3μg m−2 s−1, respectively ∼0.01 cm s−1 for the ozone deposition velocity for typical environmental conditions encountered at Summit. Uncertainty in the 30-min ozone exchange measurements (evaluated by the Monte Carlo statistical approach was on the order of 10−2 cm s−1. This uncertainty typically accounted to ~20–100% of the ozone exchange velocities that were determined. These measurements are among the most sensitive ozone deposition determinations reported to date. This flux experiment allowed for measurements of the relatively low ozone uptake rates encountered for polar snow, and thereby the study of their environmental and spring-versus-summer dependencies.

  10. Evaluation of the flux gradient technique for measurement of ozone surface fluxes over snowpack at Summit, Greenland

    Directory of Open Access Journals (Sweden)

    F. Bocquet

    2011-02-01

    Full Text Available A multi-step procedure for investigating ozone surface fluxes over polar snow by the tower gradient method was developed and evaluated. These measurements were then used to obtain four months of turbulent ozone flux data at the Summit research camp located in the center of the Greenland ice shield. Turbulent fluxes were determined by the aerodynamic gradient method incorporating tower measurements of (a ozone gradients measured by commercial ultraviolet absorption analyzers, (b ambient temperature gradients using aspirated thermocouple sensors, and (c wind speed gradients determined by cup anemometers. All gradient instruments were regularly inter-compared by bringing sensors or inlets to the same measurement height. The developed protocol resulted in an uncertainty on the order of 0.1 ppbv for 30-min averaged ozone gradients that were used for the ozone flux calculations. This protocol facilitated a lower sensitivity threshold for the ozone flux determination of −8 × 10−3 μg m−2 s−1, respectively ~0.01 cm s−1 for the ozone deposition velocity for typical environmental conditions encountered at Summit. Uncertainty in the 30-min ozone exchange measurements (evaluated by the Monte Carlo statistical approach was on the order of 10−2 cm s−1. This uncertainty typically accounted to ~20–100% of the ozone exchange velocities that were determined. These measurements are among the most sensitive ozone deposition determinations reported to date. This flux experiment, deployed at Summit for a period of four months, allowed for measurements of the relatively low ozone uptake rates encountered for polar snow, and thereby the study of their environmental and seasonal dependencies.

  11. Evaluation of the flux gradient technique for measurement of ozone surface fluxes over snowpack at Summit, Greenland

    Science.gov (United States)

    Bocquet, F.; Helmig, D.; van Dam, B. A.; Fairall, C. W.

    2011-10-01

    A multi-step procedure for investigating ozone surface fluxes over polar snow by the tower gradient method was developed and evaluated. These measurements were then used to obtain five months (April-August 2004) of turbulent ozone flux data at the Summit research camp located in the center of the Greenland ice shield. Turbulent fluxes were determined by the gradient method incorporating tower measurements of (a) ozone gradients measured by commercial ultraviolet absorption analyzers, (b) ambient temperature gradients using aspirated thermocouple sensors, and (c) wind speed gradients determined by cup anemometers. All gradient instruments were regularly inter-compared by bringing sensors or inlets to the same measurement height. The developed protocol resulted in an uncertainty on the order of 0.1 ppbv for 30-min averaged ozone gradients that were used for the ozone flux calculations. This protocol facilitated a lower sensitivity threshold for the ozone flux determination of ∼8 × 10-3μg m-2 s-1, respectively ∼0.01 cm s-1 for the ozone deposition velocity for typical environmental conditions encountered at Summit. Uncertainty in the 30-min ozone exchange measurements (evaluated by the Monte Carlo statistical approach) was on the order of 10-2 cm s-1. This uncertainty typically accounted to ~20-100% of the ozone exchange velocities that were determined. These measurements are among the most sensitive ozone deposition determinations reported to date. This flux experiment allowed for measurements of the relatively low ozone uptake rates encountered for polar snow, and thereby the study of their environmental and spring-versus-summer dependencies.

  12. A laser-induced fluorescence measurement technique for obtaining neutral hydrogen densities in plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Yao, X.Z.; Yang, T.F.; Chang-Diaz, F.R. [Plasma Fusion Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)

    1995-09-01

    The resonance fluorescence of neutral hydrogen illuminated by {ital H}{sub {alpha}} radiation has been used as a technique for the spatially and temporally resolved density measurements of neutral hydrogen in high temperature plasmas, such as in the tokamak and magnetic mirror plasma fusion devices. The fluorescence signal, usually very weak and buried in the background of stray laser light and {ital H}{sub {alpha}} emission, is very difficult to extract and its measurements are inaccurate. This paper discusses the improvement of the signal extraction using two optical path laser-induced fluorescence (LIF) methods. One optical path carries the fluorescence signal and the background (the stray laser light and {ital H}{sub {alpha}} emission), whereas the other path carries only the background signal. By combining these two signals, a clean fluorescence signal can be isolated by subtracting out the background using a differential amplifier. The measurement is obtained instantaneously from these two signals which are taken simultaneously in one pulse rather than being extracted from two separate spectra taken in two sequential pulses (double pulses). This method, therefore, makes a significant improvement on the double pulse technique in terms of the accuracy of the measurement and the time resolution. Using this LIF technique the measurement of the neutral density profile in the exhaust of a tandem mirror plasma propulsion device is obtained and presented. {copyright} {ital 1995} {ital American} {ital Institute} {ital of} {ital Physics}.

  13. Density and composition analysis using focused MeV ion mubeam techniques

    Science.gov (United States)

    Antolak, A. J.; Bench, G. S.; Pontau, A. E.; Morse, D. H.; Heikkinen, D. W.; Weirup, D. L.

    1994-12-01

    Nuclear muscopy uses focused MeV ion mubeams to non-destructively characterize materials and components with mun scale spatial resolution. Although a number of accelerator-based mubeam methods are available for materials analysis, this paper centers on the techniques of Ion mutomography (IMT) and Particle-Induced X-ray Emission (PIXE). IMT provides quantitative three-dimensional density information with mun-scale spatial resolution and 1% density variation sensitivity. Recently, IMT has become more versatile because greater emphasis has been placed on understanding the effects of reconstruction artifacts, beam spatial broadening, and limited projection data sets. PIXE provides quantitative elemental information with detection sensitivities to 1 μg/g or below in some instances. By scanning the beam, two-dimensional maps of elemental concentration can also be recorded. However, since X-rays are produced along the entire path of the ion beam as it penetrates the sample, these measurements only give depth-averaged information in general. PIXE tomography (PIXET) is the natural extension from conventional PIXE analysis to the full three-dimensional measurement and forms the bridge linking the complementary techniques of PIXE and IMT. This paper presents recent developments and applications of these ion beam techniques in a diverse range of fields including characterizing metal-matrix composites, biological specimens and inertial confinement fusion targets.

  14. Linking Soil Physical Parameters Along a Density Gradient in a Loess-Soil Long-Term Experiment

    DEFF Research Database (Denmark)

    Eden, Marie; Møldrup, Per; Schjønning, Per

    2012-01-01

    It is important to understand the impact of texture and organic carbon (OC) on soil structure development. Only few studies investigated this for silt-dominated soils. In this study, soil physical properties were determined on samples from a controlled experiment (Static Fertilization Experiment...... coefficient data; the model pore-connectivity factor was fairly constant, whereas the water blockage factor was markedly different. Water and air parameters both implied that change in bulk density was the major driver for diffusive and convective parameters in the experiment....

  15. Concept of a new technique for the combined determination of dielectric and density virial coefficients

    Science.gov (United States)

    Guenz, Christian; Gaiser, Christof; Richter, Markus

    2017-02-01

    For the combined determination of dielectric and density virial coefficients, a new measurement concept based on dielectric-constant gas thermometry (DCGT) has been elaborated. The developed apparatus design allows conventional DCGT measurements as well as gas expansion experiments to enable a distinction between density and dielectric virial coefficients. The new technique presented in this technical design note offers an independent method to obtain experimental data, which are highly desirable for the improvement of thermodynamic property models for pure gases and gas mixtures. Identification of commercial equipment and materials in this paper does not imply recommendation or endorsement by PTB, nor does it imply that the equipment and materials identified are necessarily the best available for the purpose.

  16. Bulk Crystal Growth of Piezoelectric PMN-PT Crystals Using Gradient Freeze Technique for Improved SHM Sensors

    Science.gov (United States)

    Aggarwal, Mohan D.; Kochary, F.; Penn, Benjamin G.; Miller, Jim

    2007-01-01

    There has been a growing interest in recent years in lead based perovskite ferroelectric and relaxor ferroelectric solid solutions because of their excellent dielectric, piezoelectric and electrostrictive properties that make them very attractive for various sensing, actuating and structural health monitoring (SHM) applications. We are interested in the development of highly sensitive and efficient PMN-PT sensors based on large single crystals for the structural health monitoring of composite materials that may be used in future spacecrafts. Highly sensitive sensors are needed for detection of defects in these materials because they often tend to fail by distributed and interacting damage modes and much of the damage occurs beneath the top surface of the laminate and not detectable by visual inspection. Research is being carried out for various combinations of solid solutions for PMN-PT piezoelectric materials and bigger size crystals are being sought for improved sensor applications. Single crystals of this material are of interest for sensor applications because of their high piezoelectric coefficient (d33 greater than 1700 pC/N) and electromechanical coefficients (k33 greater than 0.90). For comparison, the commonly used piezoelectric ceramic lead zirconate titanate (PZT) has a d33 of about 600 pC/N and electromechanical coefficients k33 of about 0.75. At the present time, these piezoelectric relaxor crystals are grown by high temperature flux growth method and the size of these crystals are rather small (3x4x5 mm(exp 3). In the present paper, we have attempted to grow bulk single crystals of PMN-PT in a 2 inch diameter platinum crucible and successfully grown a large size crystal of 67%PMN-33%PT using the vertical gradient freeze technique with no flux. Piezoelectric properties of the grown crystals are investigated. PMN-PT plates show excellent piezoelectric properties. Samples were poled under an applied electric field of 5 kV/cm. Dielectric properties at a

  17. The Effect of Pre-Impact Porosity and Vertical Density Gradients on the Gravity Signature of Lunar Craters

    Science.gov (United States)

    Milbury, Colleen; Johnson, Brandon C.; Melosh, H. Jay; Collins, Gareth S.; Blair, David M.; Soderblom, Jason M.; Nimmo, Francis; Phillips, Roger J.; Bierson, Carver J.; Zuber, Maria T.

    2015-11-01

    overall porosity, but higher vertical gradients, giving craters within SPA more-negative BAs than those within the highlands crust. These simulations demonstrate that the BA and porosities reported here are valid for determining general trends only.

  18. A density functional study of (17)O, (14)N and (2)H electric field gradient tensors in the real crystalline structure of alpha-glycine.

    Science.gov (United States)

    Behzadi, Hadi; Hadipour, Nasser L; Mirzaei, Mahmoud

    2007-01-01

    A density functional theory (DFT) study was carried out to calculate (17)O, (14)N and (2)H electric field gradient (EFG) tensors in accurate neutron diffraction structures of alpha-glycine at 288 and 427 K. B3LYP is the used method and 6-311+G(*) and 6-311++G(**) are the basis sets in the calculations of EFG tensors at the sites of (17)O, (14)N and (2)H nuclei in the monomer and the octameric cluster of alpha-glycine at two temperatures. Quadrupole coupling constants and asymmetry parameters are the converted parameters of calculated EFG tensors to experimentally measurable ones. The calculated results of monomer and the target molecule in octameric cluster reveal that hydrogen-bonding interactions play an important role in the crystalline structure of alpha-glycine where the results of the target molecule in octameric cluster are in good agreement with the experiments.

  19. Efficient enrichment of hepatic cancer stem-like cells from a primary rat HCC model via a density gradient centrifugation-centered method.

    Directory of Open Access Journals (Sweden)

    Wei-hui Liu

    Full Text Available BACKGROUND: Because few definitive markers are available for hepatic cancer stem cells (HCSCs, based on physical rather than immunochemical properties, we applied a novel method to enrich HCSCs. METHODOLOGY: After hepatic tumor cells (HTCs were first isolated from diethylinitrosamine-induced F344 rat HCC model using percoll discontinuous gradient centrifugation (PDGC and purified via differential trypsinization and differential attachment (DTDA, they were separated into four fractions using percoll continuous gradient centrifugation (PCGC and sequentially designated as fractions I-IV (FI-IV. Morphological characteristics, mRNA and protein levels of stem cell markers, proliferative abilities, induced differentiation, in vitro migratory capacities, in vitro chemo-resistant capacities, and in vivo malignant capacities were determined for the cells of each fraction. FINDINGS: As the density of cells increased, 22.18%, 11.62%, 4.73% and 61.47% of primary cultured HTCs were segregated in FI-FIV, respectively. The cells from FIII (density between 1.041 and 1.062 g/ml displayed a higher nuclear-cytoplasmic ratio and fewer organelles and expressed higher levels of stem cell markers (AFP, EpCAM and CD133 than cells from other fractions (P<0.01. Additionally, in vitro, the cells from FIII showed a greater capacity to self-renew, differentiate into mature HTCs, transit across membranes, close scratches, and carry resistance to chemotherapy than did cells from any other fraction; in vivo, injection of only 1×10(4 cells from FIII could generate tumors not only in subcutaneous tissue but also in the livers of nude mice. CONCLUSIONS: Through our novel method, HCSC-like cells were successfully enriched in FIII. This study will greatly contribute to two important areas of biological interest: CSC isolation and HCC therapy.

  20. Improving Soil Organic Carbon stock estimates in agricultural topsoil at a regional scale using a Stochastic Gradient Boosting technique

    Science.gov (United States)

    Schillaci, Calogero; Lombardo, Luigi; Saia, Sergio; Fantappiè, Maria; Märker, Michael; Acutis, Marco

    2016-04-01

    Agro-ecosystems have a paramount importance as a source of goods and incomes and have a highly unexpressed potential to mitigate greenhouse gasses (GHG) emission. In agro-ecosystems, Soil Organic Carbon (SOC) is recognized as the most important trait to be managed in order to maintain soil fertility and ecosystems services. Accurate laboratory analysis is indeed the best way to investigate soils. However, it is expensive and time consuming when aiming at gaining information on large areas such as an entire district or region. Remote Sensing (RS) is recently offering increasingly detailed Digital Elevation Models (DEMs) and low-cost multispectral satellite imagery. Moreover accurate worldwide climate records of the last 50 years were recently made freely available. Across Sicily, there is a strong heterogeneity of agro-ecosystems, with a dominance of field crops and orchards. In the present work, we modeled the SOC through a wide range of predictors including both ecosystem and agronomic characteristics of the soils, such as panchromatic bands, a Normalized Differenced Vegetation Index NDVI and landuse based on multispectral remote-sensed data LANDSAT ETM+7, terrain attributes derived by radar satellite data from the Shuttle Radar Topographic Mission (SRTM), as well as soil texture information and climate data record from WORLDCLIM. As dependent variable, a set of 2,891 Walkley-Black SOC and 1,049 bulk density laboratory analyses collected throughout Sicily (Italy) was used for modelling the CS stock and build the map. The Stochastic Gradient Treeboost (SGT) learning algorithm was applied to 75% of the CS stock dataset. The remaining 25% was used to validate the model. In addition, the SGT was compared to a Generalized Linear Mixed Model (GLMM). Both SGT and GLMM models show a high performance. With regards to the full model, both algorithms designated temperature and annual rainfall as fundamental predictors of CS. In addition, SGT highlighted the annual rainfall

  1. Comparison of diazo-coupling, formazan, and silver staining techniques for visualizing alkaline phosphatase isoenzymes after electrophoresis in homogeneous-pore and gradient-pore polyacrylamide gels.

    Science.gov (United States)

    Hodson, A W; Skillen, A W

    1988-03-01

    Three techniques for visualization of alkaline phosphatase after polyacrylamide-gel electrophoresis are compared. These are diazo-dye simultaneous coupling with the substrate sodium naphthyl phosphate and 5-chloro-2-toluene diazonium chloride; formazan precipitation with the substrate 5-bromo-4-chloro-3-indolyl phosphate and 3-[4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide; and silver staining with the substrate sodium glycerophosphate. Each staining technique was tested with gradient-pore and homogeneous-pore acrylamide-gel electrophoresis. The main factors assessed are sensitivity; separation of the human serum alkaline phosphatase isoenzymes of the liver, bone, and intestinal types; and differences in substrate affinity, as well as the complexity of each technique. Using the three techniques only minor differences in substrate affinity are evident. There is some nonspecific staining with the diazo-coupling technique but not with the formazan and silver staining techniques. The differences, in the mobility of the liver, bone, and intestinal isoenzymes achieved by homogeneous-pore gel electrophoresis are sufficient to allow them to be clearly distinguished. However, only very small differences in mobility are found with gradient-pore gel electrophoresis, but the sharper bands in this medium allow much smaller amounts of activity to be detected. As little as 160 microU of enzyme can be visualized by the diazo technique. Silver staining gives an approximately fourfold increase in sensitivity over the formazan technique, which in turn gives a fourfold increase over the diazo technique. An important aspect of the silver staining technique is the potential of increasing sensitivity much further by improvements in the photographic physical development stage.(ABSTRACT TRUNCATED AT 250 WORDS)

  2. Ecohydrologic controls on vegetation density and evapotranspiration partitioning across the climatic gradients of the central United States

    Directory of Open Access Journals (Sweden)

    J. P. Kochendorfer

    2008-03-01

    Full Text Available The soil-water balance and plant water use are investigated over a domain encompassing the central United States using the Statistical-Dynamical Ecohydrology Model (SDEM. The seasonality in the model and its use of the two-component Shuttleworth-Wallace canopy model allow for application of an ecological optimality hypothesis in which vegetation density, in the form of peak green leaf area index (LAI, is maximized, within upper and lower bounds, such that, in a typical season, soil moisture in the latter half of the growing season just reaches the point at which water stress is experienced. Another key feature of the SDEM is that it partitions evapotranspiration into transpiration, evaporation from canopy interception, and evaporation from the soil surface. That partitioning is significant for the soil-water balance because the dynamics of the three processes are very different. The partitioning and the model-determined peak in green LAI are validated based on observations in the literature, as well as through the calculation of water-use efficiencies with modeled transpiration and large-scale estimates of grassland productivity. Modeled-determined LAI are seen to be at least as accurate as the unaltered satellite-based observations on which they are based. Surprising little dependence on climate and vegetation type is found for the percentage of total evapotranspiration that is soil evaporation, with most of the variation across the study region attributable to soil texture and the resultant differences in vegetation density. While empirical evidence suggests that soil evaporation in the forested regions of the most humid part of the study region is somewhat overestimated, model results are in excellent agreement with observations from croplands and grasslands. The implication of model results for water-limited vegetation is that the higher (lower soil moisture content in wetter (drier climates is more-or-less completely offset by the greater

  3. Stellar acoustic radii, mean densities and ages from seismic inversion techniques

    CERN Document Server

    Buldgen, Gaël; Dupret, Marc-Antoine; Samadi, Réza

    2014-01-01

    Context. Determining stellar characteristics such as the radius, the mass or the age is crucial when studying stellar evolution, exoplanetary systems or characterising stellar populations in the Galaxy. Asteroseismology is the golden path to accurately obtain these characteristics. In this context, a key question is how to make these methods less model-dependant. Aims. Building on the work of Reese et al. (2012), we wish to extend the SOLA inversion technique to new stellar global characteristics in addition to the mean density. The goal is to provide a general framework in which to estimate these characteristics as accurately as possible in low mass main sequence stars. Methods. First, we describe our framework and discuss the reliability of the inversion technique and the possible sources of error.We then apply this methodology to the acoustic radius, an age indicator based on the sound speed derivative and the mean density and compare it to estimates based on the average large and small frequency separatio...

  4. Remote sensing and spatial statistical techniques for modelling Ommatissus lybicus (Hemiptera: Tropiduchidae) habitat and population densities.

    Science.gov (United States)

    Al-Kindi, Khalifa M; Kwan, Paul; R Andrew, Nigel; Welch, Mitchell

    2017-01-01

    In order to understand the distribution and prevalence of Ommatissus lybicus (Hemiptera: Tropiduchidae) as well as analyse their current biographical patterns and predict their future spread, comprehensive and detailed information on the environmental, climatic, and agricultural practices are essential. The spatial analytical techniques such as Remote Sensing and Spatial Statistics Tools, can help detect and model spatial links and correlations between the presence, absence and density of O. lybicus in response to climatic, environmental, and human factors. The main objective of this paper is to review remote sensing and relevant analytical techniques that can be applied in mapping and modelling the habitat and population density of O. lybicus. An exhaustive search of related literature revealed that there are very limited studies linking location-based infestation levels of pests like the O. lybicus with climatic, environmental, and human practice related variables. This review also highlights the accumulated knowledge and addresses the gaps in this area of research. Furthermore, it makes recommendations for future studies, and gives suggestions on monitoring and surveillance methods in designing both local and regional level integrated pest management strategies of palm tree and other affected cultivated crops.

  5. Microwave techniques for electron density measurements in low pressure RF plasmas

    Science.gov (United States)

    Zheltukhin, Viktor; Gafarov, Ildar; Shemakhin, Alexander

    2016-09-01

    Results of the experimental studying of RF plasma jet at low pressure in the range of 10 - 300 Pa is presented. The electron density distribution both in inductive and in capacitive coupled RF discharges was measured at 1.76 MHz and 13.56 MHz consequently. We used three independent microwave diagnostic techniques such as free space (the ``two-frequency'' and ``on the cut-off signal'') and a resonator. It is found that the electron density in the RF plasma jets is by 1-2 orders of magnitude greater than in the decaying plasma jet, and by 1-2 orders of magnitude less than in the RF plasma torch. Thus the RF plasma jet is similar to the additional discharge between the electrodes or the coil and the vacuum chamber walls. As a consequence, the formation of the positive charge sheath near the specimen placed in plasma stream is observed. It is found that the maximum of ionization degree as well as more uniform electron density distribution across the stream is observed in the range of the gas flow rate Gg = 0 . 06 - 0 . 12 g/s and the discharge power Pd = 0 . 5 - 2 . 5 kW. The work was funded by RFBR, according to the research projects No. 16-31-60081 mol_a_dk.

  6. Determination of free Zn2+ concentration in synthetic and natural samples with AGNES (Absence of Gradients and Nernstian Equilibrium Stripping) and DMT (Donnan Membrane Technique).

    Science.gov (United States)

    Chito, Diana; Weng, Liping; Galceran, Josep; Companys, Encarnació; Puy, Jaume; van Riemsdijk, Willem H; van Leeuwen, Herman P

    2012-04-01

    The determination of free Zn(2+) ion concentration is a key in the study of environmental systems like river water and soils, due to its impact on bioavailability and toxicity. AGNES (Absence of Gradients and Nernstian Equilibrium Stripping) and DMT (Donnan Membrane Technique) are emerging techniques suited for the determination of free heavy metal concentrations, especially in the case of Zn(2+), given that there is no commercial Ion Selective Electrode. In this work, both techniques have been applied to synthetic samples (containing Zn and NTA) and natural samples (Rhine river water and soils), showing good agreement. pH fluctuations in DMT and N(2)/CO(2) purging system used in AGNES did not affect considerably the measurements done in Rhine river water and soil samples. Results of DMT in situ of Rhine river water are comparable to those of AGNES in the lab. The comparison of this work provides a cross-validation for both techniques.

  7. Simulating One-Photon Absorption and Resonance Raman Scattering Spectra Using Analytical Excited State Energy Gradients within Time-Dependent Density Functional Theory

    Energy Technology Data Exchange (ETDEWEB)

    Silverstein, Daniel W.; Govind, Niranjan; van Dam, Hubertus J. J.; Jensen, Lasse

    2013-12-10

    A parallel implementation of analytical time-dependent density functional theory gradients is presented for the quantum chemistry program NWChem. The implementation is based on the Lagrangian approach developed by Furche and Ahlrichs. To validate our implementation, we first calculate the Stokes shifts for a range of organic dye molecules using a diverse set of exchange-correlation functionals (traditional density functionals, global hybrids, and range-separated hybrids) followed by simulations of the one-photon absorption and resonance Raman scattering spectrum of the phenoxyl radical, the well-studied dye molecule rhodamine 6G, and a molecular host–guest complex (TTFcCBPQT4+). The study of organic dye molecules illustrates that B3LYP and CAM-B3LYP generally give the best agreement with experimentally determined Stokes shifts unless the excited state is a charge transfer state. Absorption, resonance Raman, and fluorescence simulations for the phenoxyl radical indicate that explicit solvation may be required for accurate characterization. For the host–guest complex and rhodamine 6G, it is demonstrated that absorption spectra can be simulated in good agreement with experimental data for most exchange-correlation functionals. Finally, however, because one-photon absorption spectra generally lack well-resolved vibrational features, resonance Raman simulations are necessary to evaluate the accuracy of the exchange-correlation functional for describing a potential energy surface.

  8. Nonlinear electronic excitations in crystalline solids using meta-generalized gradient approximation and hybrid functional in time-dependent density functional theory

    Science.gov (United States)

    Sato, Shunsuke A.; Taniguchi, Yasutaka; Shinohara, Yasushi; Yabana, Kazuhiro

    2015-12-01

    We develop methods to calculate electron dynamics in crystalline solids in real-time time-dependent density functional theory employing exchange-correlation potentials which reproduce band gap energies of dielectrics; a meta-generalized gradient approximation was proposed by Tran and Blaha [Phys. Rev. Lett. 102, 226401 (2009)] (TBm-BJ) and a hybrid functional was proposed by Heyd, Scuseria, and Ernzerhof [J. Chem. Phys. 118, 8207 (2003)] (HSE). In time evolution calculations employing the TB-mBJ potential, we have found it necessary to adopt the predictor-corrector step for a stable time evolution. We have developed a method to evaluate electronic excitation energy without referring to the energy functional which is unknown for the TB-mBJ potential. For the HSE functional, we have developed a method for the operation of the Fock-like term in Fourier space to facilitate efficient use of massive parallel computers equipped with graphic processing units. We compare electronic excitations in silicon and germanium induced by femtosecond laser pulses using the TB-mBJ, HSE, and a simple local density approximation (LDA). At low laser intensities, electronic excitations are found to be sensitive to the band gap energy: they are close to each other using TB-mBJ and HSE and are much smaller in LDA. At high laser intensities close to the damage threshold, electronic excitation energies do not differ much among the three cases.

  9. Nonlinear electronic excitations in crystalline solids using meta-generalized gradient approximation and hybrid functional in time-dependent density functional theory

    Energy Technology Data Exchange (ETDEWEB)

    Sato, Shunsuke A. [Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba 305-8571 (Japan); Taniguchi, Yasutaka [Center for Computational Science, University of Tsukuba, Tsukuba 305-8571 (Japan); Department of Medical and General Sciences, Nihon Institute of Medical Science, 1276 Shimogawara, Moroyama-Machi, Iruma-Gun, Saitama 350-0435 (Japan); Shinohara, Yasushi [Max Planck Institute of Microstructure Physics, 06120 Halle (Germany); Yabana, Kazuhiro [Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba 305-8571 (Japan); Center for Computational Science, University of Tsukuba, Tsukuba 305-8571 (Japan)

    2015-12-14

    We develop methods to calculate electron dynamics in crystalline solids in real-time time-dependent density functional theory employing exchange-correlation potentials which reproduce band gap energies of dielectrics; a meta-generalized gradient approximation was proposed by Tran and Blaha [Phys. Rev. Lett. 102, 226401 (2009)] (TBm-BJ) and a hybrid functional was proposed by Heyd, Scuseria, and Ernzerhof [J. Chem. Phys. 118, 8207 (2003)] (HSE). In time evolution calculations employing the TB-mBJ potential, we have found it necessary to adopt the predictor-corrector step for a stable time evolution. We have developed a method to evaluate electronic excitation energy without referring to the energy functional which is unknown for the TB-mBJ potential. For the HSE functional, we have developed a method for the operation of the Fock-like term in Fourier space to facilitate efficient use of massive parallel computers equipped with graphic processing units. We compare electronic excitations in silicon and germanium induced by femtosecond laser pulses using the TB-mBJ, HSE, and a simple local density approximation (LDA). At low laser intensities, electronic excitations are found to be sensitive to the band gap energy: they are close to each other using TB-mBJ and HSE and are much smaller in LDA. At high laser intensities close to the damage threshold, electronic excitation energies do not differ much among the three cases.

  10. MR assessment of left ventricular function: quantitative comparison of fast imaging employing steady-state acquisition (FIESTA) with fast gradient echo cine technique.

    Science.gov (United States)

    Li, Wei; Stern, Jessica S; Mai, Vu M; Pierchala, Linda N; Edelman, Robert R; Prasad, Pottumarthi V

    2002-11-01

    To evaluate the agreement of fast imaging employing steady-state acquisition (FIESTA) cine technique with segmented k-space fast gradient echo (GRE) cine technique when using them for assessment of cardiac function. Eleven MR cine studies were performed on six healthy volunteers and five patients, using FIESTA and fast GRE techniques. The quantitative measurements of ventricular function obtained from the two techniques were compared. The data analysis was performed by two observers independently. Compared to fast GRE cine technique, FIESTA cine technique consistently resulted in higher end-diastolic volume (10.2%) and end-systolic volume (21.6%), but lower myocardial mass of left ventricle (19.2%) and ejection fraction (9.9%). The stroke volume obtained from the two techniques was very close. The primary explanation for this variability is that the two techniques have different mechanisms on establishing signal contrast. Compared to fast GRE technique, FIESTA provides significantly different results when using it for assessment of left ventricular function. It is important to consider this difference in the assessment of cardiac function. Copyright 2002 Wiley-Liss, Inc.

  11. Comparison of volatility function technique for risk-neutral densities estimation

    Science.gov (United States)

    Bahaludin, Hafizah; Abdullah, Mimi Hafizah

    2017-08-01

    Volatility function technique by using interpolation approach plays an important role in extracting the risk-neutral density (RND) of options. The aim of this study is to compare the performances of two interpolation approaches namely smoothing spline and fourth order polynomial in extracting the RND. The implied volatility of options with respect to strike prices/delta are interpolated to obtain a well behaved density. The statistical analysis and forecast accuracy are tested using moments of distribution. The difference between the first moment of distribution and the price of underlying asset at maturity is used as an input to analyze forecast accuracy. RNDs are extracted from the Dow Jones Industrial Average (DJIA) index options with a one month constant maturity for the period from January 2011 until December 2015. The empirical results suggest that the estimation of RND using a fourth order polynomial is more appropriate to be used compared to a smoothing spline in which the fourth order polynomial gives the lowest mean square error (MSE). The results can be used to help market participants capture market expectations of the future developments of the underlying asset.

  12. Density of states techniques for lattice field theories using the functional fit approach (FFA)

    CERN Document Server

    Gattringer, Christof; Lehmann, Alexander; Törek, Pascal

    2015-01-01

    We discuss a variant of density of states (DoS) techniques for lattice field theories, the so-called "functional fit approach" (FFA). The DoS FFA is based on a density of states rho(x) which is parameterized on small intervals of the argument x of rho(x). On these intervals restricted Monte Carlo simulations with an additional Boltzmann factor exp(lambda x) allow to determine rho(x) very precisely by obtaining its parameters from fitting the Monte Carlo data to a known function of lambda. We describe the method in detail and show its applicability in four different systems, three of which have a complex action problem: The SU(3) spin model with a chemical potential, U(1) lattice gauge theory, the Z(3) spin model with chemical potential, and 2-dimensional U(1) lattice gauge theory with a topological term. In all cases we compare to reference calculations, which partly were done in a dual formulation where the complex action problem is absent. In all four cases we find a very encouraging performance of the DoS ...

  13. Effect of resination technique on mechanical properties of medium density fiberboard

    Directory of Open Access Journals (Sweden)

    Nadir Ayrilmis

    2013-02-01

    Full Text Available Effects of different resination techniques relative to the mechanical properties of commercially produced thick medium density fiberboard (MDF were investigated. The amount of urea-formaldehyde resin (11 wt% applied to the wood fibers was gradually decreased in the blowline (11, 10.5, 10, and 9.5 wt%, while it was gradually increased in the short-retention blender (0, 0.5, 1, and 1.5. wt%. The internal bond strength of the MDF boards considerably improved as the amount of the resin applied to the fibers in the short-retention blender was increased to 1.5 wt%. In particular, the increase in the IB strength was most pronounced as the resin content increased from 1 to 1.5%. The edge and face screw withdrawal resistances increased by 7.7 and 7.9% as the amount of the resin applied to the fibers in the blender was increased. Similar values were also observed for the flexural properties. Overall, the total resin content in the production of thick MDF can be decreased as blender resination, a means of post-dryer resin addition, is incorporated into the blowline resination technique.

  14. Applications of Low Density Flow Techniques and Catalytic Recombination at the Johnson Space Center

    Science.gov (United States)

    Scott, Carl D.

    2000-01-01

    The talk presents a brief background on defInitions of catalysis and effects associated with chemically nonequilibrium and low-density flows of aerospace interest. Applications of catalytic recombination on surfaces in dissociated flow are given, including aero heating on reentry spacecraft thermal protection surfaces and reflection of plume flow on pressure distributions associated with the space station. Examples include aero heating predictions for the X-38 test vehicle, the inlet of a proposed gas-sampling probe used in high enthalpy test facilities, and a parabolic body at angle of attack. The effect of accommodation coefficients on thruster induced pressure distributions is also included. Examples of tools used include simple aero heating formulas based on boundary layer solutions, an engineering approximation that uses axisymmetric viscous shock layer flow to simulate full three dimensional flow, full computational fluid dynamics, and direct simulation Monte-Carlo calculations. Methods of determining catalytic recombination rates in arc jet flow are discus ed. An area of catalysis not fully understood is the formation of single-wall carbon nanotubes (SWNT) with gas phase or nano-size metal particles. The Johnson Space Center is making SWNTs using both a laser ablation technique and an electric arc vaporization technique.

  15. The Effect of Pre-Impact Porosity and Vertical Density Gradients on the Gravity Signature of Lunar Craters as Seen by GRAIL

    Science.gov (United States)

    Milbury, C.; Johnson, B. C.; Melosh, H., IV; Collins, G. S.; Blair, D. M.; Soderblom, J. M.; Nimmo, F.; Bierson, C. J.; Phillips, R. J.; Zuber, M. T.

    2015-12-01

    As a result of NASA's dual spacecraft Gravity Recovery And Interior Laboratory (GRAIL) mission [Zuber et al., 2013; doi:10.1126/science.1231507], we now know that the lunar crust is highly porous and that the porosity varies laterally [Wieczorek et al., 2013; doi:10.1126/science.1231530] and vertically [Besserer et al., 2014; doi:10.1002/2014GL060240]. Analysis of complex craters located within the lunar highlands reveals that: 1) craters larger than diameter D~210 have positive Bouguer Anomalies (BAs), 2) craters with D ≲ 100 km have both positive and negative BAs that vary about the (near 0) mean by approximately ± 25 mGal, and, 3) D and BA are anticorrelated for craters with D ≲ 100 km [Soderblom et al., 2015; submitted]. Numerical modeling by Milbury et al. [2015, LPSC] shows that pre-impact porosity is the dominant influence on the gravity signature of complex craters with D ≲ 100 km, and mantle uplift dominates the gravity for those with D > 140 km. Phillips et al. [2015, LPSC] showed that complex craters located in the South Pole-Aitken (SPA) basin tend to have more-negative BAs than similar craters in the highlands. By including (pre-impact) vertical porosity/density gradients in our impact simulations, we reproduce the observed anticorrelation between BA and D for D ≲ 100 km, and the observed difference between the BAs of SPA and highland craters. We use the iSALE hydrocode including pore space compaction [Wünnemann et al., 2006; doi:10.1016/j.icarus.2005.10.013] and dilatant bulking [Collins, 2014; doi:10.1002/2014JE004708] to understand how the gravity signature of impact craters develop. In this study we vary density/porosity with depth. We find that simulations that have constant porosity with depth have a lower BA for a given crater diameter than those with varying porosity. We used two different mean porosities (7% and 14%) and found that the BA increases with increasing porosity, similar to simulations with constant porosity. Larger

  16. Investigation on the growth and characterization of 4-aminobenzophenone single crystal by the vertical dynamic gradient freeze technique

    Science.gov (United States)

    Prabhakaran, SP.; Ramesh Babu, R.; Sukumar, M.; Bhagavannarayana, G.; Ramamurthi, K.

    2014-03-01

    Growth of bulk single crystal of 4-Aminobenzophenone (4-ABP) from the vertical dynamic gradient freeze (VDGF) setup designed with eight zone furnace was investigated. The experimental parameters for the growth of 4-ABP single crystal with respect to the design of VDGF setup are discussed. The eight zones were used to generate multiple temperature gradients over the furnace, and video imaging system helped to capture the real time growth and solid-liquid interface. 4-ABP single crystal with the size of 18 mm diameter and 40 mm length was grown from this investigation. Structural and optical quality of grown crystal was examined by high resolution X-ray diffraction and UV-visible spectral analysis, respectively and the blue emission was also confirmed from the photoluminescence spectrum. Microhardness number of the crystal was estimated at different loads using Vicker's microhardness tester. The size and quality of single crystal grown from the present investigation are compared with the vertical Bridgman grown 4-ABP.

  17. Calorific value of Prosopis africana and Balanites aegyptiaca wood: Relationships with tree growth, wood density and rainfall gradients in the West African Sahel

    Energy Technology Data Exchange (ETDEWEB)

    Montes, Carmen Sotelo; Weber, John C. [World Agroforestry Centre (ICRAF), Sahel Office, B.P. E 5118 Bamako (Mali); Silva, Dimas Agostinho da; Bolzon de Muniz, Graciela Ines [Universidade Federal do Parana (UFPR), Av. Lothario Meissner, 900, CEP.: 80270-170-Curitiba (Brazil); Garcia, Rosilei A. [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Instituto de Florestas, Departamento de Produtos Florestais, BR 465, km 07, 23890-000, Seropedica, Rio de Janeiro (Brazil)

    2011-01-15

    Prosopis africana and Balanites aegyptiaca are native tree species in the West African Sahel and provide wood for fuel, construction and other essential products. A provenance/progeny test of each species was established at one relatively dry site in Niger, and evaluated at 13 years. Gross calorific value of the wood was determined for a random sample of trees in each test: gross CV and CVm{sup 3} = gross calorific value in MJ kg{sup -1} and MJ m{sup -3}, respectively. The major objectives were to determine if gross CV was positively correlated with wood density and tree growth, and if gross CV and/or CVm{sup 3} varied with rainfall gradients in the sample region. Provenances were grouped into a drier and more humid zone, and correlations were computed among all trees and separately in each zone. Results indicated that gross CV was not significantly correlated with density in either species. Gross CV was positively correlated with growth of P. africana (but not B. aegyptiaca) only in the drier zone. Gross CVm{sup 3} was positively correlated with growth of both species, and the correlations were stronger in the drier zone. Multiple regressions with provenance latitude, longitude and elevation indicated that provenance means for gross CV increased, in general, from the drier to the more humid zones. Regressions with gross CVm{sup 3} were not significant. Results are compared with earlier research reports from the provenance/progeny tests and with other tropical hardwood species; and practical implications are presented for tree improvement and conservation programs in the region. (author)

  18. Sperm selection by Capripure(®) density-gradient centrifugation versus the dextran swim-up procedure in wild mountain ruminants.

    Science.gov (United States)

    Santiago-Moreno, J; Esteso, M C; Castaño, C; Toledano-Díaz, A; Rodríguez, E; López-Sebastián, A

    2014-10-01

    This study compares the effectiveness of two methods of sperm selection - Capripure(®) density-gradient centrifugation (DGC) and dextran swim-up (DSU) - in semen samples from Iberian ibex (Capra pyrenaica) and European mouflon (Ovis musimon). During the increasing photoperiod, Capripure(®) DGC improved the percentage of sperm with progressive motility (Pmouflon, Capripure(®) DGC selection was unaffected by photoperiod, had no influence on any sperm variable, and selected 47.8% of the initial total number of mouflon spermatozoa in ejaculate samples. Photoperiod had no influence on the effectiveness of DSU in either ibexes or mouflons. In the ibexes, DSU reduced (Pmouflons, DSU had no significant influence on any sperm variable, and selected 27.8% of the initial total number. Capripure(®) DGC improved ibex and mouflon sperm motility (Pmouflon, sperm cells showing non-progressive motility were found after only 20 h of post-centrifugation incubation following Capripure(®) DGC selection. In conclusion, Capripure(®) DGC would seem a useful method for selecting the best spermatozoa from both ibex and mouflon ejaculates.

  19. [Development of a gentamicin producer under different culture conditions studied by a method of differential centrifugation of the mycelium in a saccharose density gradient].

    Science.gov (United States)

    Losev, V V; Laznikova, T N; Dmitrieva, S V

    1981-05-01

    The method of differential centrifugation in the sucrose density gradient (SDG) enabled one to trace the changes in the development of the seed and fermentation mycelium of the gentamicin-producing organism. Correlation between gentamicin distribution in the SDG and the culture productivity was found. It was shown that the culture grown under the optimal aeration and agitation conditions was characterized by formation of higher amounts of the mycelium in the 5th and 6th layers of the SDG. Such mycelium was more productive than that from the other SDG layers. The most productive 48-hour seed culture had the more significant part of the mycelium in the 3rd layer of the SDG. When such a culture had the more significant part of the mycelium in the 3rd layer of the SDG. When such a culture was used as the seed material, the activity of the fermentation broth was the highest. The method of differential centrifugation in the SDG provides determination of the culture productivity by the volumes of the fermentation mycelium in the 5th and 6th layers or the seed mycelium in the 3rd layer of the SDG.

  20. Instability of spiral and scroll waves in the presence of a gradient in the fibroblast density: the effects of fibroblast-myocyte coupling

    CERN Document Server

    Zimik, Soling

    2016-01-01

    Fibroblast-myocyte coupling can modulate electrical-wave dynamics in cardiac tissue. In diseased hearts, the distribution of fibroblasts is heterogeneous, so there can be gradients in the fibroblast density (henceforth we call this GFD) especially from highly injured regions, like infarcted or ischemic zones, to less-wounded regions of the tissue. Fibrotic hearts are known to be prone to arrhythmias, so it is important to understand the effects of GFD in the formation and sustenance of arrhythmic re- entrant waves, like spiral or scroll waves. Therefore, we investigate the effects of GFD on the stability of spiral and scroll waves of electrical activation in a state-of-the- art mathematical model for cardiac tissue in which we also include fibroblasts. By introducing GFD in controlled ways, we show that spiral and scroll waves can be unstable in the presence of GFDs because of regions with varying spiral or scroll-wave frequency {\\omega}, induced by the GFD. We examine the effects of the resting membrane pote...

  1. Density gradient theory combined with the PC-SAFT equation of state used for modeling the surface tension of associating systems

    Directory of Open Access Journals (Sweden)

    Vinš Václav

    2014-03-01

    Full Text Available The density gradient theory (GT combined with a SAFT-type (Statistical Associating Fluid Theory equation of state has been used for modeling the surface tension of associating fluids represented by a series of six alkanols ranging from methanol to 1-pentanol. The effect of nonzero dipole moment of the selected alkanols on the predicted surface tension was investigated in this study. Results of the GT + non-polar Perturbed Chain (PC SAFT equation of state were compared to predictions of GT combined with the PC-polar-SAFT, i.e. PCP-SAFT, equation. Both GT + PC-SAFT and GT + PCP-SAFT give reasonable prediction of the surface tension for pure alkanols. Results of both models are comparable as no significant difference in the modeled saturation properties and in the predicted surface tension using GT was found. Consideration of dipolar molecules of selected alkanols using PCP-SAFT had only minor effect on the predicted properties compared to the non-polar PC-SAFT model.

  2. Zinc phthalocyanine thin film and chemical analyte interaction studies by density functional theory and vibrational techniques.

    Science.gov (United States)

    Saini, G S S; Singh, Sukhwinder; Kaur, Sarvpreet; Kumar, Ranjan; Sathe, Vasant; Tripathi, S K

    2009-06-03

    Thin films of zinc phthalocyanine have been deposited on KBr and glass substrates by the thermal evaporation method and characterized by the x-ray diffraction, optical, infrared and Raman techniques. The observed x-ray diffraction and infrared absorption spectra of as-deposited thin films suggest the presence of an α crystalline phase. Infrared and Raman spectra of thin films after exposure to vapours of ammonia and methanol have also been recorded. Shifts in the position of some IR and Raman bands in the spectra of exposed films have been observed. Some bands also show changes in their intensity on exposure. Increased charge on the phthalocyanine ring and out-of-plane distortion of the core due to interaction between zinc phthalocyanine and vapour molecules involving the fifth coordination site of the central metal ion may be responsible for the band shifts. Changes in the intensity of bands are interpreted in terms of the lowering of molecular symmetry from D(4h) to C(4v) due to doming of the core. Molecular parameters and Mulliken atomic charges of zinc phthalocyanine and its complexes with methanol and ammonia have been calculated from density functional theory. The binding energy of the complexes have also been calculated. Calculated values of the energy for different complexes suggest that axially coordinated vapour molecules form the most stable complex. Calculated Mulliken atomic charges show net charge transfer from vapour molecules to the phthalocyanine ring for the most stable complex.

  3. TBI lung dose comparisons using bilateral and anteroposterior delivery techniques and tissue density corrections.

    Science.gov (United States)

    Bailey, Daniel W; Wang, Iris Z; Lakeman, Tara; Hales, Lee D; Singh, Anurag K; Podgorsak, Matthew B

    2015-03-08

    This study compares lung dose distributions for two common techniques of total body photon irradiation (TBI) at extended source-to-surface distance calculated with, and without, tissue density correction (TDC). Lung dose correction factors as a function of lateral thorax separation are approximated for bilateral opposed TBI (supine), similar to those published for anteroposterior-posteroanterior (AP-PA) techniques in AAPM Report 17 (i.e., Task Group 29). 3D treatment plans were created retrospectively for 24 patients treated with bilateral TBI, and for whom CT data had been acquired from the head to the lower leg. These plans included bilateral opposed and AP-PA techniques- each with and without - TDC, using source-to-axis distance of 377 cm and largest possible field size. On average, bilateral TBI requires 40% more monitor units than AP-PA TBI due to increased separation (26% more for 23 MV). Calculation of midline thorax dose without TDC leads to dose underestimation of 17% on average (standard deviation, 4%) for bilateral 6 MV TBI, and 11% on average (standard deviation, 3%) for 23 MV. Lung dose correction factors (CF) are calculated as the ratio of midlung dose (with TDC) to midline thorax dose (without TDC). Bilateral CF generally increases with patient separation, though with high variability due to individual uniqueness of anatomy. Bilateral CF are 5% (standard deviation, 4%) higher than the same corrections calculated for AP-PA TBI in the 6 MV case, and 4% higher (standard deviation, 2%) for 23 MV. The maximum lung dose is much higher with bilateral TBI (up to 40% higher than prescribed, depending on patient anatomy) due to the absence of arm tissue blocking the anterior chest. Dose calculations for bilateral TBI without TDC are incorrect by up to 24% in the thorax for 6 MV and up to 16% for 23 MV. Bilateral lung CF may be calculated as 1.05 times the values published in Table 6 of AAPM Report 17, though a larger patient pool is necessary to better

  4. Antimicrobial susceptibility testing of Shiga toxin-producing Escherichia coli from various samples by using a spiral gradient endpoint technique.

    Science.gov (United States)

    Khatiwada, Janak; Fullerton, Marjorie; Davis, Shurrita; Williams, Leonard L

    2012-01-01

    Shiga toxin-producing Escherichia coli (STEC) remains a major public health concern. Microbial resistance may be due to use of antimicrobial agents (AAs) as a growth promoter in food animals or overuse of AAs in humans. The objective of the current study was to determine the antimicrobial susceptibility patterns of STEC strains isolated from food, veterinary, and clinical sources against 14 AAs by using the spiral gradient endpoint method. One hundred ten isolates from three sources were characterized. Results of the current study showed that all strains were resistant to the folate pathway inhibiting AAs including tylosin tartrate (gradient minimum inhibitory concentration [GMIC] ranges from ≥180.00 to 256.00 μg/mL; end concentration [EC] ranges from ≥130.00 to 151.22 μg/mL; and tail-end concentration [TEC] ≥145.00 μg/mL). All the strains isolated from three sources were susceptible to the fluoroquinolone class of AAs (GMIC ranges from ≤1.00 to 64.30 μg/mL; EC ranges from ≤3.33 to 72.00 μg/mL; and TEC ranges from ≤12.13 to 45.00 μg/mL). Among the food isolates, less resistance was found within the aminoglycoside and amphenicol group (GMIC ≥256.00 μg/mL; EC=161.00 μg/mL). Eight strains were resistant to one to three, 44 strains were resistant to four to six, and two strains were resistant to seven or more AAs. All the clinical isolates (100%) were susceptible to the fluoroquinolones and gentamycin. Results also showed that antimicrobial resistance was observed between four and six AAs among the isolates. Some veterinary isolates were resistant to five AAs. Least AAs resistance was shown by 3.7% of isolates to gentamycin and 7.45% to chloramphenicol. This study showed an increasing trend of antimicrobial resistant strains of STEC, and we suggest that periodic surveillance of the antimicrobial susceptibility may be a useful measure to detect the antimicrobial resistant pathogens.

  5. Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation

    Directory of Open Access Journals (Sweden)

    Jun Takezawa

    2010-01-01

    Full Text Available When a replicative DNA polymerase stalls upon encountering a lesion on the template strand, it is relieved by other low-processivity polymerase(s, which insert nucleotide(s opposite the lesion, extend by a few nucleotides, and dissociate from the 3′-OH. The replicative polymerase then resumes DNA synthesis. This process, termed translesion replication (TLS or replicative bypass, may involve at least five different polymerases in mammals, although the participating polymerases and their roles have not been entirely characterized. Using siRNAs originally designed and an alkaline sucrose density gradient sedimentation technique, we verified the involvement of several polymerases in ultraviolet (UV light-induced TLS in HeLa cells. First, siRNAs to Rev3 or Rev7 largely abolished UV-TLS, suggesting that these 2 gene products, which comprise Polζ, play a main role in mutagenic TLS. Second, Rev1-targeted siRNA also abrogated UV-TLS, indicating that Rev1 is also indispensable to mutagenic TLS. Third, Polη-targeted siRNA also prevented TLS to a greater extent than our expectations. Forth, although siRNA to Polι had no detectable effect, that to Polκ delayed UV-TLS. To our knowledge, this is the first study reporting apparent evidence for the participation of Polκ in UV-TLS.

  6. Surface Tension of Binary Mixtures Including Polar Components Modeled by the Density Gradient Theory Combined with the PC-SAFT Equation of State

    Science.gov (United States)

    Vinš, Václav; Planková, Barbora; Hrubý, Jan

    2013-05-01

    In this study, the Cahn-Hilliard density gradient theory (GT) is used for predicting the surface tension of various binary mixtures at relatively wide temperature ranges and for testing the application of the GT for predictions of homogeneous nucleation. The GT was combined with two physically based equations of state (EoS), namely the perturbed-chain (PC) statistical associating fluid theory (SAFT) and its modification for polar substances the perturbed-chain polar (PCP) SAFT. The GT applied to the planar phase interface was employed to predict the interfacial tension for various quadrupolar (CO2 and benzene) and dipolar (difluoromethane, i.e., R32; pentafluoroethane, i.e., R125; and 1,1,1,2-tetrafluoroethane, i.e., R134a) substances and for five binary mixtures including polar components ( n-decane + CO2, benzene + CO2, R32 + R125, R32 + R134a, R134a + R125). The PCP-SAFT EoS combined with the GT provides more accurate results for both the quadrupolar and dipolar substances than the original PC-SAFT EoS. Besides the planar phase interface, the GT was also applied to the spherical phase interface simulating a critical cluster occurring in homogeneous nucleation of droplets. Carbon dioxide was considered, because it has a relatively high quadrupole moment and because of its relevance to natural gas processing. Application of the PCP-SAFT EoS provides a significant improvement compared to the PC-SAFT EoS, and it is clearly superior to the classical cubic Peng-Robinson EoS, which is still used for modeling droplet nucleation.

  7. Comparison of the effect of different media on the clinical outcomes of the density-gradient centrifugation/swim-up and swim-up methods.

    Science.gov (United States)

    Kim, Eun-Kyung; Kim, Eun-Ha; Kim, Eun-Ah; Lee, Kyung-Ah; Shin, Ji-Eun; Kwon, Hwang

    2015-03-01

    Sperm must be properly prepared in in vitro fertilization (IVF)-embryo transfer (ET) programs in order to control the fertilization rate and ensure that embryos are of high quality and have appropriate developmental abilities. The objective of this study was to determine the most optimal sperm preparation method for IVF. Patients less than 40 years of age who participated in a fresh IVF-ET cycle from November 2012 to March 2013 were included in this study. Poor responders with less than three mature oocytes were excluded. Ham's F-10 medium or sperm-washing medium (SWM) was used in combination with the density-gradient centrifugation/swim-up (DGC-SUP) or SUP methods for sperm preparation. A total of 429 fresh IVF-ET cycles were grouped according to the media and methods used for sperm preparation and retrospectively analyzed (DGC-SUP/Ham's F-10, n=82; DGC-SUP/SWM, n=43; SUP/Ham's F-10, n=181; SUP/SWM, n=123). There were no significant differences among these four groups with respect to the mean age of the female partners, duration of infertility, number of previous IVF cycles, and retrieved oocytes. We determined that both the DGC-SUP and SUP methods for sperm preparation from whole semen, using either Ham's F-10 or SWM media, result in comparable clinical outcomes, including fertilization and pregnancy rates. We suggest that both media and both methods for sperm preparation can be used for selecting high-quality sperm for assistive reproductive technology programs.

  8. Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting.

    Science.gov (United States)

    Chi, Hee-Jun; Kwak, Su-Jin; Kim, Seok-Gi; Kim, Youn-Young; Park, Ji-Young; Yoo, Chang-Seok; Park, Il-Hae; Sun, Hong-Gil; Kim, Jae-Won; Lee, Kyeong-Ho

    2016-12-01

    This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency. Semen analysis and a sperm DNA fragmentation assay were performed to assess the correlations between semen parameters and the DFI in 458 semen samples. Sperm with progressive motility or non-apoptosis were isolated by DGC or MACS, respectively, in 29 normozoospermic semen samples. The effects of DGC or MACS alone and of DGC and MACS combined on reducing the amount of sperm in the sample with DNA fragmentation and protamine deficiency were investigated. The sperm DFI showed a significant correlation (r=-0.347, p<0.001) with sperm motility and morphology (r=-0.114, p<0.05) but not with other semen parameters. The DFI (11.5%±2.0%) of semen samples was significantly reduced by DGC (8.1%±4.1%) or MACS alone (7.4%±3.9%) (p<0.05). The DFI was significantly further reduced by a combination of DGC and MACS (4.1%±1.3%, p<0.05). Moreover, the combination of DGC and MACS (1.6%±1.1%, p<0.05) significantly reduced the protamine deficiency rate of semen samples compared to DGC (4.4%±3.2%) or MACS alone (3.4%±2.2%). The combination of DGC and MACS may be an effective method to isolate high-quality sperm with progressive motility, non-apoptosis, high DNA integrity, and low protamine deficiency in clinical use.

  9. A novel consistent photomicrography technique using a reference slide made of neutral density filter.

    Science.gov (United States)

    Kim, Jong Yeob; Kim, Ji Woong; Seo, Soo Hong; Kye, Young Chul; Ahn, Hyo Hyun

    2011-05-01

    Obtaining consistent photomicrographic images of pathology slides is not always easy because of many different types and settings of the equipment such as the microscopes and digital cameras. In this study, we developed a photomicrography technique that could acquire consistent images of pathology slides. The neutral density (ND) filter was attached to a transparent glass slide as a reference slide, photographed using consistent settings, and acquired images that harbored all of the areas of gray, white, and black. In the same way, the slide was replaced by the actual pathology slide, and photomicrographed. To simulate different light environment, the above photographic session was repeated using two different light intensities and microscopes. A graphic program was used to adjust levels of the reference slide images and this leveling, or calibration, was saved as a file for each. This file for leveling process was applied to actual subsequent photomicrographic images. The same sites of noncalibrated and calibrated images of the pathology slide were calculated into CIELAB or CIE L*a*b* coordinates. Then, the color differences (ΔE*ab) were calculated. As results, in the study using a 50% transmittance ND filter, two original different images were made nearly identical to the unaided eye, especially in two-point (white and gray) and three-point (black, white, and gray) leveling. In comparison of different light intensities, the ΔE*ab of the selected area was 0.9 in two-point leveling. Between different microscopes, 10.7 of ΔE*ab was the smallest value in three-point leveling. This method would be helpful for acquiring consistent photomicrographic images of pathology slides.

  10. Automated Method for Analysis of Mammographic Breast Density - A Technique for Breast Cancer Risk Estimation

    Science.gov (United States)

    2006-07-01

    based texture analysis to generate an orientation image, mean shift smoothing, and ridge-tracking. For a data set of 118 MLO view mammograms, the new...tion. The data set comprised the four- view craniocaudal (CC) and medio - lateral oblique (MLO) mammograms obtained in 65 patients who were ran- domly...structures, and then a gradient-based texture analysis was designed to extract a texture orientation image that represented the dominant texture orientation

  11. SU-E-T-470: Importance of HU-Mass Density Calibration Technique in Proton Pencil Beam Dose Calculation

    Energy Technology Data Exchange (ETDEWEB)

    Penfold, S; Miller, A [University of Adelaide, Adelaide, SA (Australia)

    2015-06-15

    Purpose: Stoichiometric calibration of Hounsfield Units (HUs) for conversion to proton relative stopping powers (RStPs) is vital for accurate dose calculation in proton therapy. However proton dose distributions are not only dependent on RStP, but also on relative scattering power (RScP) of patient tissues. RScP is approximated from material density but a stoichiometric calibration of HU-density tables is commonly neglected. The purpose of this work was to quantify the difference in calculated dose of a commercial TPS when using HU-density tables based on tissue substitute materials and stoichiometric calibrated ICRU tissues. Methods: Two HU-density calibration tables were generated based on scans of the CIRS electron density phantom. The first table was based directly on measured HU and manufacturer quoted density of tissue substitute materials. The second was based on the same CT scan of the CIRS phantom followed by a stoichiometric calibration of ICRU44 tissue materials. The research version of Pinnacle{sup 3} proton therapy was used to compute dose in a patient CT data set utilizing both HU-density tables. Results: The two HU-density tables showed significant differences for bone tissues; the difference increasing with increasing HU. Differences in density calibration table translated to a difference in calculated RScP of −2.5% for ICRU skeletal muscle and 9.2% for ICRU femur. Dose-volume histogram analysis of a parallel opposed proton therapy prostate plan showed that the difference in calculated dose was negligible when using the two different HU-density calibration tables. Conclusion: The impact of HU-density calibration technique on proton therapy dose calculation was assessed. While differences were found in the calculated RScP of bony tissues, the difference in dose distribution for realistic treatment scenarios was found to be insignificant.

  12. Maximum voltage gradient technique for optimization of ablation for typical atrial flutter with zero-fluoroscopy approach.

    Science.gov (United States)

    Deutsch, Karol; Śledź, Janusz; Mazij, Mariusz; Ludwik, Bartosz; Labus, Michał; Karbarz, Dariusz; Pasicka, Bernadetta; Chrabąszcz, Michał; Śledź, Arkadiusz; Klank-Szafran, Monika; Vitali-Sendoz, Laura; Kameczura, Tomasz; Śpikowski, Jerzy; Stec, Piotr; Ujda, Marek; Stec, Sebastian

    2017-06-01

    Radiofrequency catheter ablation (RFCA) is an established effective method for the treatment of typical cavo-tricuspid isthmus (CTI)-dependent atrial flutter (AFL). The introduction of 3-dimensional electro-anatomic systems enables RFCA without fluoroscopy (No-X-Ray [NXR]). The aim of this study was to evaluate the feasibility and effectiveness of CTI RFCA during implementation of the NXR approach and the maximum voltage-guided (MVG) technique for ablation of AFL.Data were obtained from prospective standardized multicenter ablation registry. Consecutive patients with the first RFCA for CTI-dependent AFL were recruited. Two navigation approaches (NXR and fluoroscopy based as low as reasonable achievable [ALARA]) and 2 mapping and ablation techniques (MVG and pull-back technique [PBT]) were assessed. NXR + MVG (n  =  164; age: 63.7 ± 9.5; 30% women), NXR + PBT (n  =  55; age: 63.9 ± 10.7; 39% women); ALARA + MVG (n  =  36; age: 64.2 ± 9.6; 39% women); and ALARA + PBT (n  =  205; age: 64.7 ± 9.1; 30% women) were compared, respectively. All groups were simplified with a 2-catheter femoral approach using 8-mm gold tip catheters (Osypka AG, Germany or Biotronik, Germany) with 15 min of observation. The MVG technique was performed using step-by-step application by mapping the largest atrial signals within the CTI.Bidirectional block in CTI was achieved in 99% of all patients (P  =  NS, between groups). In NXR + MVG and NXR + PBT groups, the procedure time decreased (45.4 ± 17.6 and 47.2 ± 15.7 min vs. 52.6 ± 23.7 and 59.8 ± 24.0 min, P < .01) as compared to ALARA + MVG and ALARA + PBT subgroups. In NXR + MVG and NXR + PBT groups, 91% and 98% of the procedures were performed with complete elimination of fluoroscopy. The NXR approach was associated with a significant reduction in fluoroscopy exposure (from 0.2 ± 1.1 [NXR + PBT] and 0.3 ± 1.6 [NXR + MVG] to 7.7 ± 6.0 min [ALARA + MVG] and 9

  13. Interpretation of Source Parameters from Total Gradient of Gravity and Magnetic Anomalies Caused by Thin Dyke using Nonlinear Global Optimization Technique

    Science.gov (United States)

    Biswas, A.

    2016-12-01

    A proficient way to deal with appraisal model parameters from total gradient of gravity and magnetic data in light of Very Fast Simulated Annealing (VFSA) has been exhibited. This is the first run through of applying VFSA in deciphering total gradient of potential field information with another detailing estimation brought on because of detached causative sources installed in the subsurface. The model parameters translated here are the amplitude coefficient (k), accurate origin of causative source (x0) depth (z0) and the shape factor (q). The outcome of VFSA improvement demonstrates that it can exceptionally decide all the model parameters when shape variable is fixed. The model parameters assessed by the present strategy, for the most part the shape and depth of the covered structures was observed to be in astounding concurrence with the genuine parameters. The technique has likewise the capability of dodging very uproarious information focuses and enhances the understanding results. Investigation of Histogram and cross-plot examination likewise proposes the translation inside the assessed ambiguity. Inversion of noise-free and noisy synthetic data information for single structures and field information shows the viability of the methodology. The procedure has been carefully and adequately connected to genuine field cases (Leona Anomaly, Senegal for gravity and Pima copper deposit, USA for magnetic) with the nearness of mineral bodies. The present technique can be to a great degree material for mineral investigation or ore bodies of dyke-like structure rooted in the shallow and more deep subsurface. The calculation time for the entire procedure is short.

  14. A new technique to measure the neutralizer cell gas line density applied to a DIII-D neutral beamline

    Energy Technology Data Exchange (ETDEWEB)

    Kessler, D.N.; Hong, R.M.; Riggs, S.P.

    1995-10-01

    The DIII-D tokamak employs eight ion sources for plasma heating. In order to obtain the maximum neutralization of energetic ions (providing maximum neutral beam power) and reduce the heat load on beamline internal components caused by residual energetic ions, sufficient neutral gas must be injected into the beamline neutralizer cell. The neutral gas flow rate must be optimized, however, since excessive gas will increase power losses due to neutral beam scattering and reionization. It is important, therefore, to be able to determine the neutralizer cell gas line density. A new technique which uses the ion source suppressor grid current to obtain the neutralizer cell gas line density has been developed. The technique uses the fact that slow ions produced by beam-gas interactions in the neutralizer cell during beam extraction are attracted to the negative potential applied to the suppressor grid, inducing current flow in the grid. By removing the dependence on beam energy and beam current a normalized suppressor grid current function can be formed which is dependent only on the gas line density. With this technique it is possible to infer the gas line density on a shot by shot basis.

  15. Self-anti-reflective density-modulated thin films by HIPS technique

    Science.gov (United States)

    Keles, Filiz; Badradeen, Emad; Karabacak, Tansel

    2017-08-01

    A critical factor for an efficient light harvesting device is reduced reflectance in order to achieve high optical absorptance. In this regard, refractive index engineering becomes important to minimize reflectance. In this study, a new fabrication approach to obtain density-modulated CuIn x Ga(1-x)Se2 (CIGS) thin films with self-anti-reflective properties has been demonstrated. Density-modulated CIGS samples were fabricated by utilizing high pressure sputtering (HIPS) at Ar gas pressure of 2.75 × 10-2 mbar along with conventional low pressure sputtering (LPS) at Ar gas pressure of 3.0 × 10-3 mbar. LPS produces conventional high density thin films while HIPS produces low density thin films with approximate porosities of ˜15% due to a shadowing effect originating from the wide-spread angular atomic of HIPS. Higher pressure conditions lower the film density, which also leads to lower refractive index values. Density-modulated films that incorporate a HIPS layer at the side from which light enters demonstrate lower reflectance thus higher absorptance compared to conventional LPS films, although there is not any significant morphological difference between them. This result can be attributed to the self-anti-reflective property of the density-modulated samples, which was confirmed by the reduced refractive index calculated for HIPS layer via an envelope method. Therefore, HIPS, a simple and scalable approach, can provide enhanced optical absorptance in thin film materials and eliminate the need for conventional light trapping methods such as anti-reflective coatings of different materials or surface texturing.

  16. Optimisation of coal blend and bulk density for coke ovens by vibrocompacting technique non-recovery ovens

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, P.P.; Vinoo, D.S.; Yadav, U.S.; Ghosh, S.; Lal, J.P.N. [J.S.W. Steel Ltd, Bellary (India)

    2007-09-15

    The quality of coke produced in a coke oven depends on the coal blend characteristics and carbonisation conditions. Scarcity of good quality coking coal made it necessary to look for techniques capable of producing superior coke from inferior coals. Precarbonisation techniques improve the bulk density of the coal charge and produce good quality coke from inferior coals. The stamp charging technique, the most effective among them requires finer crushing of coal and higher moisture as binder, both requiring additional energy. JSW Steel has adopted vibrocompaction along with non-recovery ovens for its 1.2 Mtpa coke production. This is a highly ecofriendly coke making process producing excellent quality coke from inferior coals. It increases the bulk density of cake, similar to stamp charging, using compaction in place of stamping. A cake density of 1.10 t m{sup -3} has been achieved using the vibrocompacting technique with optimum moisture and crushing fineness. Coal blend containing up to 35% soft coal and coking coal, having 32% volatile matter have been successfully used to produce a coke with coke strength after reaction >65%, coke reactivity index <25% and M10 <6%. The paper discusses the experience of operating vibrocompaction non-recovery coke ovens.

  17. Selective evaluation of high density lipoprotein from mouse small intestine by an in situ perfusion technique[S

    Science.gov (United States)

    Yamaguchi, Satoshi; Zhang, Bo; Tomonaga, Takeshi; Seino, Utako; Kanagawa, Akiko; Segawa, Masaru; Nagasaka, Hironori; Suzuki, Akira; Miida, Takashi; Yamada, Sohsuke; Sasaguri, Yasuyuki; Doi, Takefumi; Saku, Keijiro; Okazaki, Mitsuyo; Tochino, Yoshihiro; Hirano, Ken-ichi

    2014-01-01

    The small intestine (SI) is the second-greatest source of HDL in mice. However, the selective evaluation of SI-derived HDL (SI-HDL) has been difficult because even the origin of HDL obtained in vivo from the intestinal lymph duct of anesthetized rodents is doubtful. To shed light on this question, we have developed a novel in situ perfusion technique using surgically isolated mouse SI, with which the possible filtration of plasma HDL into the SI lymph duct can be prevented. With the developed method, we studied the characteristics of and mechanism for the production and regulation of SI-HDL. Nascent HDL particles were detected in SI lymph perfusates in WT mice, but not in ABCA1 KO mice. SI-HDL had a high protein content and was smaller than plasma HDL. SI-HDL was rich in TG and apo AIV compared with HDL in liver perfusates. SI-HDL was increased by high-fat diets and reduced in apo E KO mice. In conclusion, with our in situ perfusion model that enables the selective evaluation of SI-HDL, we demonstrated that ABCA1 plays an important role in intestinal HDL production, and SI-HDL is small, dense, rich in apo AIV, and regulated by nutritional and genetic factors. PMID:24569139

  18. Mercury Distribution in the Deûle River (Northern France) Measured by the Diffusive Gradients in Thin Films Technique and Conventional Methods.

    Science.gov (United States)

    Diviš, Pavel; Kadlecová, Milada; Ouddane, Baghdad

    2016-05-01

    The distribution of mercury in surface water and in sediment from Deûle River in Northern France was studied by application of conventional sampling methods and by diffusive gradients in thin films technique (DGT). Concentration of total dissolved mercury in surface water was 20.8 ± 0.8 ng l(-1). The particulate mercury concentration was 6.2 ± 0.6 µg g(-1). The particulate mercury was accumulated in sediment (9.9 ± 2.3 mg kg(-1)), and it was transformed by methylating bacteria to methylmercury, mainly in the first 2-cm layer of the sediment. Total dissolved concentration of mercury in sediment pore water obtained by application of centrifugation extraction was 17.6 ± 4.1 ng l(-1), and it was comparable with total dissolved pore water mercury concentration measured by DGT probe containing Duolite GT-73 resin gel (18.2 ± 4.3 ng l(-1)), taking the sediment heterogeneity and different principles of the applied methods into account. By application of two DGT probes with different resin gels specific for mercury, it was found that approximately 30% of total dissolved mercury in sediment pore water was present in labile forms easy available for biota. The resolution of mercury DGT depth profiles was 0.5 cm, which allows, unlike conventional techniques, to study the connection of the geochemical cycle of mercury with geochemical cycles of iron and manganese.

  19. Comparison of high latitude electron density profiles obtained with the GPS radio occultation technique and EISCAT measurements

    Directory of Open Access Journals (Sweden)

    C. Stolle

    2004-06-01

    Full Text Available To obtain a comprehensive view on high latitude processes by applying different observation techniques, the SIRCUS campaign was initiated in 2001/2002. This paper compares electron density profiles derived from CHAMP radio occultation data and those measured with the EISCAT facility. Since ionospheric profiling with the help of space-based received GPS is a relatively new technique, validations with established independent instruments are of crucial need. We present 28 profiling events for quasi-statistical analyses, which occurred during the SIRCUS campaigns and describe some of them in more detail. We found out that the majority of profile comparisons in electron density peak value and height, as well as in TEC, lie within the error ranges of the two methods. Differences in the ionospheric quantities do not necessarily occur when the locations of the occultation and of the radar site show considerable distances. Differences are more pronounced when the ionosphere is remarkably structured.

  20. A Method to Improve Electron Density Measurement of Cone-Beam CT Using Dual Energy Technique

    Directory of Open Access Journals (Sweden)

    Kuo Men

    2015-01-01

    Full Text Available Purpose. To develop a dual energy imaging method to improve the accuracy of electron density measurement with a cone-beam CT (CBCT device. Materials and Methods. The imaging system is the XVI CBCT system on Elekta Synergy linac. Projection data were acquired with the high and low energy X-ray, respectively, to set up a basis material decomposition model. Virtual phantom simulation and phantoms experiments were carried out for quantitative evaluation of the method. Phantoms were also scanned twice with the high and low energy X-ray, respectively. The data were decomposed into projections of the two basis material coefficients according to the model set up earlier. The two sets of decomposed projections were used to reconstruct CBCT images of the basis material coefficients. Then, the images of electron densities were calculated with these CBCT images. Results. The difference between the calculated and theoretical values was within 2% and the correlation coefficient of them was about 1.0. The dual energy imaging method obtained more accurate electron density values and reduced the beam hardening artifacts obviously. Conclusion. A novel dual energy CBCT imaging method to calculate the electron densities was developed. It can acquire more accurate values and provide a platform potentially for dose calculation.

  1. Novel analysis technique for measuring edge density fluctuation profiles with reflectometry in the Large Helical Device

    Science.gov (United States)

    Creely, A. J.; Ida, K.; Yoshinuma, M.; Tokuzawa, T.; Tsujimura, T.; Akiyama, T.; Sakamoto, R.; Emoto, M.; Tanaka, K.; Michael, C. A.

    2017-07-01

    A new method for measuring density fluctuation profiles near the edge of plasmas in the Large Helical Device (LHD) has been developed utilizing reflectometry combined with pellet-induced fast density scans. Reflectometer cutoff location was calculated by proportionally scaling the cutoff location calculated with fast far infrared laser interferometer (FIR) density profiles to match the slower time resolution results of the ray-tracing code LHD-GAUSS. Plasma velocity profile peaks generated with this reflectometer mapping were checked against velocity measurements made with charge exchange spectroscopy (CXS) and were found to agree within experimental uncertainty once diagnostic differences were accounted for. Measured density fluctuation profiles were found to peak strongly near the edge of the plasma, as is the case in most tokamaks. These measurements can be used in the future to inform inversion methods of phase contrast imaging (PCI) measurements. This result was confirmed with both a fixed frequency reflectometer and calibrated data from a multi-frequency comb reflectometer, and this method was applied successfully to a series of discharges. The full width at half maximum of the turbulence layer near the edge of the plasma was found to be only 1.5-3 cm on a series of LHD discharges, less than 5% of the normalized minor radius.

  2. Uncertainty quantification techniques for population density estimates derived from sparse open source data

    Science.gov (United States)

    Stewart, Robert; White, Devin; Urban, Marie; Morton, April; Webster, Clayton; Stoyanov, Miroslav; Bright, Eddie; Bhaduri, Budhendra L.

    2013-05-01

    The Population Density Tables (PDT) project at Oak Ridge National Laboratory (www.ornl.gov) is developing population density estimates for specific human activities under normal patterns of life based largely on information available in open source. Currently, activity-based density estimates are based on simple summary data statistics such as range and mean. Researchers are interested in improving activity estimation and uncertainty quantification by adopting a Bayesian framework that considers both data and sociocultural knowledge. Under a Bayesian approach, knowledge about population density may be encoded through the process of expert elicitation. Due to the scale of the PDT effort which considers over 250 countries, spans 50 human activity categories, and includes numerous contributors, an elicitation tool is required that can be operationalized within an enterprise data collection and reporting system. Such a method would ideally require that the contributor have minimal statistical knowledge, require minimal input by a statistician or facilitator, consider human difficulties in expressing qualitative knowledge in a quantitative setting, and provide methods by which the contributor can appraise whether their understanding and associated uncertainty was well captured. This paper introduces an algorithm that transforms answers to simple, non-statistical questions into a bivariate Gaussian distribution as the prior for the Beta distribution. Based on geometric properties of the Beta distribution parameter feasibility space and the bivariate Gaussian distribution, an automated method for encoding is developed that responds to these challenging enterprise requirements. Though created within the context of population density, this approach may be applicable to a wide array of problem domains requiring informative priors for the Beta distribution.

  3. Uncertainty Quantification Techniques for Population Density Estimates Derived from Sparse Open Source Data

    Energy Technology Data Exchange (ETDEWEB)

    Stewart, Robert N [ORNL; White, Devin A [ORNL; Urban, Marie L [ORNL; Morton, April M [ORNL; Webster, Clayton G [ORNL; Stoyanov, Miroslav K [ORNL; Bright, Eddie A [ORNL; Bhaduri, Budhendra L [ORNL

    2013-01-01

    The Population Density Tables (PDT) project at the Oak Ridge National Laboratory (www.ornl.gov) is developing population density estimates for specific human activities under normal patterns of life based largely on information available in open source. Currently, activity based density estimates are based on simple summary data statistics such as range and mean. Researchers are interested in improving activity estimation and uncertainty quantification by adopting a Bayesian framework that considers both data and sociocultural knowledge. Under a Bayesian approach knowledge about population density may be encoded through the process of expert elicitation. Due to the scale of the PDT effort which considers over 250 countries, spans 40 human activity categories, and includes numerous contributors, an elicitation tool is required that can be operationalized within an enterprise data collection and reporting system. Such a method would ideally require that the contributor have minimal statistical knowledge, require minimal input by a statistician or facilitator, consider human difficulties in expressing qualitative knowledge in a quantitative setting, and provide methods by which the contributor can appraise whether their understanding and associated uncertainty was well captured. This paper introduces an algorithm that transforms answers to simple, non-statistical questions into a bivariate Gaussian distribution as the prior for the Beta distribution. Based on geometric properties of the Beta distribution parameter feasibility space and the bivariate Gaussian distribution, an automated method for encoding is developed that responds to these challenging enterprise requirements. Though created within the context of population density, this approach may be applicable to a wide array of problem domains requiring informative priors for the Beta distribution.

  4. Development of a spectroscopic technique for simultaneous magnetic field, electron density, and temperature measurements in ICF-relevant plasmas

    Science.gov (United States)

    Dutra, E. C.; Koch, J. A.; Presura, R.; Angermeier, W. A.; Darling, T.; Haque, S.; Mancini, R. C.; Covington, A. M.

    2016-11-01

    Spectroscopic techniques in the visible range are often used in plasma experiments to measure B-field induced Zeeman splitting, electron densities via Stark broadening, and temperatures from Doppler broadening. However, when electron densities and temperatures are sufficiently high, the broadening of the Stark and Doppler components can dominate the emission spectra and obscure the Zeeman component. In this research, we are developing a time-resolved multi-axial technique for measuring the Zeeman, Stark, and Doppler broadened line emission of dense magnetized plasmas for Z-pinch and Dense Plasma Focus (DPF) accelerators. The line emission is used to calculate the electron densities, temperatures, and B-fields. In parallel, we are developing a line-shape modeling code that incorporates the broadening effects due to Stark, Doppler, and Zeeman effects for dense magnetized plasma. This manuscript presents the details of the experimental setup and line shape code, along with the results obtained from an Al iii doublet at the University of Nevada, Reno at Nevada Terawatt Facility. Future tests are planned to further evaluate the technique and modeling on other material wire array, gas puff, and DPF platforms.

  5. "Prospecting Asteroids: Indirect technique to estimate overall density and inner composition"

    Science.gov (United States)

    Such, Pamela

    2016-07-01

    Spectroscopic studies of asteroids make possible to obtain some information on their composition from the surface but say little about the innermost material, porosity and density of the object. In addition, spectroscopic observations are affected by the effects of "space weathering" produced by the bombardment of charged particles for certain materials that change their chemical structure, albedo and other physical properties, partly altering their chances of identification. Data such as the mass, size and density of the asteroids are essential at the time to propose space missions in order to determine the best candidates for space exploration and is of great importance to determine a priori any of them remotely from Earth. From many years ago its determined masses of largest asteroids studying the gravitational effects they have on smaller asteroids when they approach them (see Davis and Bender, 1977; Schubart and Matson, 1979; School et al 1987; Hoffman, 1989b, among others), but estimates of the masses of the smallest objects is limited to the effects that occur in extreme close encounters to other asteroids of similar size. This paper presents the results of a search for approaches of pair of asteroids that approximate distances less than 0.0004 UA (50,000 km) of each other in order to study their masses through the astrometric method and to estimate in a future their densities and internal composition. References Davis, D. R., and D. F. Bender. 1977. Asteroid mass determinations: search for futher encounter opportunities. Bull. Am. Astron. Soc. 9, 502-503. Hoffman, M. 1989b. Asteroid mass determination: Present situation and perspectives. In asteroids II (R. P. Binzel, T. Gehreis, and M. S. Matthews, Eds.), pp 228-239. Univ. Arizona Press, Tucson. School, H. L. D. Schmadel and S. Roser 1987. The mass of the asteroid (10) Hygiea derived from observations of (829) Academia. Astron. Astrophys. 179, 311-316. Schubart, J. And D. L. Matson 1979. Masses and

  6. Preparation and characterization of chemical gradient surfaces and their application for the study of cellular interaction phenomena

    NARCIS (Netherlands)

    Ruardy, TG; Schakenraad, JM; vanderMei, HC; Busscher, HJ

    1997-01-01

    Chemical gradient surfaces are surfaces with a gradually changing chemistry along their length which is responsible for a position bound variation in physical properties, most notably, the wettability. In this review, methods to prepare (palladium deposition, diffusion technique, density gradient me

  7. Preparation and characterization of chemical gradient surfaces and their application for the study of cellular interaction phenomena

    NARCIS (Netherlands)

    Ruardy, TG; Schakenraad, JM; vanderMei, HC; Busscher, HJ

    1997-01-01

    Chemical gradient surfaces are surfaces with a gradually changing chemistry along their length which is responsible for a position bound variation in physical properties, most notably, the wettability. In this review, methods to prepare (palladium deposition, diffusion technique, density gradient me

  8. Advantageous use of SSA technique to observe effects of thickness, antioxidant and oxygen in gamma irradiated low density polyethylene

    Energy Technology Data Exchange (ETDEWEB)

    Perez, C.J., E-mail: cjperez@fi.mdp.edu.ar [Research Institute of Material Science and Technology (INTEMA), National Research Council (CONICET), Engineering Faculty, Mar del Plata University, Av. J.B. Justo 4302, 7600 Mar del Plata (Argentina); Failla, M.D. [Planta Piloto de Ingenieria Quimica-PLAPIQUI (UNS-CONICET), Camino ' La Carrindanga' Km 7, 8000 Bahia Blanca (Argentina); Carella, J.M. [Research Institute of Material Science and Technology (INTEMA), National Research Council (CONICET), Engineering Faculty, Mar del Plata University, Av. J.B. Justo 4302, 7600 Mar del Plata (Argentina)

    2012-06-20

    Highlights: Black-Right-Pointing-Pointer Information from successive self-nucleation and annealing technique is analyzed. Black-Right-Pointing-Pointer Oxygen and antioxidants reduce crosslinking efficiency by reaction with free radicals. Black-Right-Pointing-Pointer Recognizable differences are obtained in samples irradiated at different atmospheres. - Abstract: Information obtained from successive self-nucleation and annealing (SSA) technique is analyzed, paying special attention to the observable effects of samples thickness and antioxidant and oxygen concentrations. Molecular structure changes for low density polyethylene (LDPE) samples, irradiated under three different atmospheres for doses between 33 and 222 kGy were analyzed, with emphasis on the changes of longer polymethylene crystallizable lengths. Antioxidant and oxygen concentrations were varied for samples of different thickness to study the effects on degradation. The changes in the molecular structure were followed simultaneously by SSA and Infrared spectroscopy (FTIR) via carbonyl group concentration. Preliminary quantifications of the SSA technique sensitivity are also advanced.

  9. Gradient networks

    Science.gov (United States)

    Toroczkai, Zoltán; Kozma, Balázs; Bassler, Kevin E.; Hengartner, N. W.; Korniss, G.

    2008-04-01

    Gradient networks are defined (Toroczkai and Bassler 2004 Nature 428 716) as directed graphs formed by local gradients of a scalar field distributed on the nodes of a substrate network G. We present the derivation for some of the general properties of gradient graphs and give an exact expression for the in-degree distribution R(l) of the gradient network when the substrate is a binomial (Erd{\\;\\kern -0.10em \\raise -0.35ex \\{{^{^{\\prime\\prime}}}}\\kern -0.57em \\o} s-Rényi) random graph, G_{N,p} , and the scalars are independent identically distributed (i.i.d.) random variables. We show that in the limit N \\to \\infty, p \\to 0, z = pN = \\mbox{const} \\gg 1, R(l)\\propto l^{-1} for l Bassler (2004 Nature 428 716).

  10. Laser textured surface gradients

    Science.gov (United States)

    Ta, Van Duong; Dunn, Andrew; Wasley, Thomas J.; Li, Ji; Kay, Robert W.; Stringer, Jonathan; Smith, Patrick J.; Esenturk, Emre; Connaughton, Colm; Shephard, Jonathan D.

    2016-05-01

    This work demonstrates a novel technique for fabricating surfaces with roughness and wettability gradients and their subsequent applications for chemical sensors. Surface roughness gradients on brass sheets are obtained directly by nanosecond laser texturing. When these structured surfaces are exposed to air, their wettability decreases with time (up to 20 days) achieving both spatial and temporal wettability gradients. The surfaces are responsive to organic solvents. Contact angles of a series of dilute isopropanol solutions decay exponentially with concentration. In particular, a fall of 132° in contact angle is observed on a surface gradient, one order of magnitude higher than the 14° observed for the unprocessed surface, when the isopropanol concentration increased from 0 to 15.6 wt%. As the wettability changes gradually over the surface, contact angle also changes correspondingly. This effect offers multi-sensitivity at different zones on the surface and is useful for accurate measurement of chemical concentration.

  11. Atmospheric density, temperature and wind measurement techniques during the 1980 Energy Budget Campaign

    Science.gov (United States)

    Schmidlin, F. J.; Philbrick, C. R.; Offermann, D.

    1981-01-01

    Super Loki rocketsonde systems are described. The datasonde telemeters data to a ground station where ambient temperatures are calculated between 20 and 70 km. The sphere is a passive, radar tracked system which allows density to be calculated between 30 and 90 km. When flown simultaneously the systems give redundant data in the altitudes between 30 and 70 km. The datasonde has a balloon parachute that descends more slowly than a conventional parachute and is more stable. Because of launch constraints the datasondes reached very high apogees, leading to very fast descent velocities. Aerodynamic heating reduced thermistor sensitivity. Anomalous parachute behavior influenced wind sensing until a denser layer was reached. The spheres collapsed above 60 km altitude, but their data, combined with dropsonde data give significant results for 21 flights. These show that the stratosphere is colder than Cospar 72 model predictions and the mesosphere is warmer.

  12. Deriving the electron-phonon spectral density of MgB2 from optical data, using maximum entropy techniques.

    Science.gov (United States)

    Hwang, J; Carbotte, J P

    2014-04-23

    We use maximum entropy techniques to extract an electron-phonon density from optical data for the normal state at T = 45 K of MgB2. Limiting the analysis to a range of phonon energies below 110 meV, which is sufficient for capturing all phonon structures, we find a spectral function that is in good agreement with that calculated for the quasi-two-dimensional σ-band. Extending the analysis to higher energies, up to 160 meV, we find no evidence for any additional contributions to the fluctuation spectrum, but find that the data can only be understood if the density of states is taken to decrease with increasing energy.

  13. An investigation of density measurement method for yarn-dyed woven fabrics based on dual-side fusion technique

    Science.gov (United States)

    Zhang, Rui; Xin, Binjie

    2016-08-01

    Yarn density is always considered as the fundamental structural parameter used for the quality evaluation of woven fabrics. The conventional yarn density measurement method is based on one-side analysis. In this paper, a novel density measurement method is developed for yarn-dyed woven fabrics based on a dual-side fusion technique. Firstly, a lab-used dual-side imaging system is established to acquire both face-side and back-side images of woven fabric and the affine transform is used for the alignment and fusion of the dual-side images. Then, the color images of the woven fabrics are transferred from the RGB to the CIE-Lab color space, and the intensity information of the image extracted from the L component is used for texture fusion and analysis. Subsequently, three image fusion methods are developed and utilized to merge the dual-side images: the weighted average method, wavelet transform method and Laplacian pyramid blending method. The fusion efficacy of each method is evaluated by three evaluation indicators and the best of them is selected to do the reconstruction of the complete fabric texture. Finally, the yarn density of the fused image is measured based on the fast Fourier transform, and the yarn alignment image could be reconstructed using the inverse fast Fourier transform. Our experimental results show that the accuracy of density measurement by using the proposed method is close to 99.44% compared with the traditional method and the robustness of this new proposed method is better than that of conventional analysis methods.

  14. An ensemble average method to estimate absolute TEC using radio beacon-based differential phase measurements: Applicability to regions of large latitudinal gradients in plasma density

    Science.gov (United States)

    Thampi, Smitha V.; Bagiya, Mala S.; Chakrabarty, D.; Acharya, Y. B.; Yamamoto, M.

    2014-12-01

    A GNU Radio Beacon Receiver (GRBR) system for total electron content (TEC) measurements using 150 and 400 MHz transmissions from Low-Earth Orbiting Satellites (LEOS) is fabricated in house and made operational at Ahmedabad (23.04°N, 72.54°E geographic, dip latitude 17°N) since May 2013. This system receives the 150 and 400 MHz transmissions from high-inclination LEOS. The first few days of observations are presented in this work to bring out the efficacy of an ensemble average method to convert the relative TECs to absolute TECs. This method is a modified version of the differential Doppler-based method proposed by de Mendonca (1962) and suitable even for ionospheric regions with large spatial gradients. Comparison of TECs derived from a collocated GPS receiver shows that the absolute TECs estimated by this method are reliable estimates over regions with large spatial gradient. This method is useful even when only one receiving station is available. The differences between these observations are discussed to bring out the importance of the spatial differences between the ionospheric pierce points of these satellites. A few examples of the latitudinal variation of TEC during different local times using GRBR measurements are also presented, which demonstrates the potential of radio beacon measurements in capturing the large-scale plasma transport processes in the low-latitude ionosphere.

  15. Separation of single-walled carbon nanotubes by gel-based chromatography using surfactant step-gradient techniques and development of new instrumentation for studying SWCNT reaction processes

    Science.gov (United States)

    Breindel, Leonard M.

    Single-walled carbon nanotube (SWCNT) synthesis methods such as CoMoCATTM, HiPcoTM, pulsed laser vaporization (PLV), and catalytic chemical vapor deposition (CCVD) produce several different distributions of (n,m) SWCNT structures, where ( n,m) defines the nanotube diameter and chiral wrapping angle. Post-synthesis processing such as functionalization and/or separations must therefore be employed to yield high purity electronic or single (n,m) samples. Through the use of a surfactant gradient across a gel-based chromatographic column, separations of single (n,m) species can be achieved. Anionic surfactants such as SDS, SDBS, and AOT display different separation effectiveness for single (n,m) species. Results of near-infrared optical absorption for separated SWCNT surfactant suspensions will be discussed, leading to a broader understanding of the important factors necessary for the gel chromatography separation technique. In particular, the effects of SWCNT/surfactant micelle structure are found to be key to achieving fast, simple SWCNT electronic type separations. Additionally, development of new instrumentation for the near-infrared spectrofluorimetric analysis (NIR-SFA) of SWCNTs is useful to the advancement of fundamental SWCNT research and applications. NIR-SFA, for instance, allows for the (n,m) structures of a sample to be identified and monitored during the progress of a chemical reaction or separation experiment. Seeking to achieve the time resolutions necessary for such experiments, the design and optimizations of a system utilizing single-wavelength excitation by diode lasers coupled with a fast NIR detection system are presented.

  16. LDRD ER Final Report: Recreating Planetary Cores in the Laboratory: New Techniques to Extremely High Density States

    Energy Technology Data Exchange (ETDEWEB)

    Collins, G; Celliers, P; Hicks, D; Cauble, R; Bradley, D; MacKinnon, A; Moon, S; Young, D; Chau, R; Eggert, J; Willi, P; Pasley, J; Jeanloz, R; Lee, K; Bennedetti, R; Koenig, M; Benuzzi-Mounaix, A; Batani, D; Loubeyre, P; Hubbard, W

    2003-02-07

    An accurate equation of state (EOS) for planetary constituents at extreme conditions is the key to any credible model of planets or low mass stars. However, very few materials have their high pressure (>few Mbar) EOS experimentally validated, and even then, only on the principal Hugoniot. For planetary and stellar interiors, compression occurs from gravitational force so that material states follow a line of isotropic compression (ignoring phase separation) to ultra-high densities. An example of the hydrogen phase space composing Jupiter and one particular Brown Dwarf is shown. At extreme densities, material states are predicted to have quite unearthly properties such as high temperature superconductivity and low temperature fusion. High density experiments on Earth are achieved with either static compression techniques (i.e. diamond anvil cells) or dynamic compression techniques using large laser facilities, gas guns, or explosives. The ultimate goal of this multi-directorate and multi-institutional proposal was to develop techniques that will enable us to understand material states that previously only existed at the core of giant planets, stars, or speculative theories. Our effort was a complete success, meeting all of the objectives set out in our proposals. First we focused on developing accurate Hugoniot techniques to be used for constraining the equation of state at high pressure/temperature. We mapped out an accurate water EOS and measured that the ionic->electronic conduction transition occurs at lower pressures than models predict. These data and their impact are fully described in the first enclosed paper ''The Equation of State and Optical Properties of Water Compressed by Strong Shock Waves.'' Currently models used to construct planetary isentropes are constrained by only the planet radius, outer atmospheric spectroscopy, and space probe gravitational moment and magnetic field data. Thus these data, which provide rigid constraints

  17. Distribution and density of mast cells in camel small intestine and influence of fixation techniques

    Directory of Open Access Journals (Sweden)

    MB Al-Zghoul

    2009-08-01

    Full Text Available This study was carried out to gather species-specific data on mast-cell density and distribution in camel small intestine under different fixation conditions and to elucidate the presence and cross-reactivity of tryptase in the camel small intestine using human specific anti-tryptase antibody. Tissue specimens from the jejunum, duodenum, and ileum were obtained from 9 healthy, 9-12 months old, male camels. Specimens were fixed either with carnoy’s fluid or formalinbuffered solution and stained with either methylene blue or immunohistochemically to identify mast cells. The present study demonstrated for the first time, the presence and cross-reactivity of tryptase in the camel small intestine using a specific mouse anti-human tryptase antibody. Mast cells were detected in all histological layers of the camel small intestine (mucosal, submucosal, muscularis externa and serosa. Among all locations examined in the duodenum, ileum and jejunum, no significant difference was observed in mast-cell counts among the lamina propria, muscularis mucosae, muscularis externa and the serosa. The only significant difference observed was the mast-cell count in submucosa region where the highest and lowest mast count was observed in the jejenual and ileal submucosa, respectively. Significant differences regarding the distribution of mast cell as well as the influence of the fixation method could be observed. This underlines the fact that data regarding mast cell heterogeneity from other species, obtained by different fixation methods, are not comparable. This fact has to be taken into account when evaluating mast cell subtypes under pathological conditions.

  18. Influence of Heartwood on Wood Density and Pulp Properties Explained by Machine Learning Techniques

    Directory of Open Access Journals (Sweden)

    Carla Iglesias

    2017-01-01

    Full Text Available The aim of this work is to develop a tool to predict some pulp properties e.g., pulp yield, Kappa number, ISO brightness (ISO 2470:2008, fiber length and fiber width, using the sapwood and heartwood proportion in the raw-material. For this purpose, Acacia melanoxylon trees were collected from four sites in Portugal. Percentage of sapwood and heartwood, area and the stem eccentricity (in N-S and E-W directions were measured on transversal stem sections of A. melanoxylon R. Br. The relative position of the samples with respect to the total tree height was also considered as an input variable. Different configurations were tested until the maximum correlation coefficient was achieved. A classical mathematical technique (multiple linear regression and machine learning methods (classification and regression trees, multi-layer perceptron and support vector machines were tested. Classification and regression trees (CART was the most accurate model for the prediction of pulp ISO brightness (R = 0.85. The other parameters could be predicted with fair results (R = 0.64–0.75 by CART. Hence, the proportion of heartwood and sapwood is a relevant parameter for pulping and pulp properties, and should be taken as a quality trait when assessing a pulpwood resource.

  19. Fabrication of polystyrene hollow microspheres as laser fusion targets by optimized density-matched emulsion technique and characterization

    Indian Academy of Sciences (India)

    K K Mishra; R K Khardekar; Rashmi Singh; H C Pant

    2002-07-01

    Inertial confinement fusion, frequently referred to as ICF, inertial fusion, or laser fusion, is a means of producing energy by imploding small hollow microspheres containing thermonuclear fusion fuel. Polymer microspheres, which are used as fuel containers, can be produced by solution-based micro-encapsulation technique better known as density-matched emulsion technique. The specifications of these microspheres are very rigorous, and various aspects of the emulsion hydrodynamics associated with their production are important in controlling the final product. This paper describes about the optimization of various parameters associated with density-matched emulsion method in order to improve the surface smoothness, wall thickness uniformity and sphericity of hollow polymer microspheres. These polymer microshells have been successfully fabricated in our lab, with 3–30 m wall thickness and 50–1600 m diameters. The sphericity and wall thickness uniformity are better than 99%. Elimination of vacuoles and high yield rate has been achieved by adopting the step-wise heating of W1/O/W2 emulsion for solvent removal.

  20. Analysing the effect of stand density and site conditions on structure and growth of oak species using Nelder trials along an environmental gradient: experimental design, evaluation methods, and results

    Institute of Scientific and Technical Information of China (English)

    Enno; Uhl; Peter; Biber; Matthias; Ulbricht; Michael; Heym; Tamás; Horváth; Ferenc; Lakatos; Janós; Gál; Leonhard; Steinacker; Giustino; Tonon; Maurizio; Ventura; Hans; Pretzsch

    2015-01-01

    Background: Most current approaches in forest science and practice require information about structure and growth of individual trees rather than- or in addition to- sum and mean values of growth and yield at forest stand level as provided by classic experimental designs. By inventing the wheel design, Nelder provided the possibility to turn to the individual tree as basic information unit. Such trials provide valuable insights into the dependency of growth on stand density at particular sites.Methods: Here, we present an extension of the original design and evaluation by Nelder.(i) We established Nelder wheels along an environmental gradient through Europe in atlantic climate in Belgium and Germany, Mediterranean climate in Italy, continental climate in Hungary as well as on high land climate in Mexico. Such disjunct Nelder wheels along an environmental gradient can be regarded and analysed as a two-factor design with the factors of site condition and stand density.(ii) We present an advanced statistical approach to evaluate density dependent growth dynamics of trees planted in form of the Nelder design, which considers spatio-temporal autocorrelation.(iii)We prove the usefulness of the methods in improving ecological theory concerning density related productivity,trade-offs between facilitation and competition, and allometric relations between size variables.Results: First evaluations based on remeasured Nelder wheels in oak(Quercus robur L.) show a size growth differentiation during the first observation period. In particular, height growth is accelerated under higher competition indicating facilitation effects. We detect furthermore a high variability in allometric relations.Conclusions: The proposed design, methods, and results are discussed regarding their impact on forest practice,model building, and ecological theory. We conclude that the extended Nelder approach is highly efficient in providing currently lacking individual tree level information.

  1. Analysing the effect of stand density and site conditions on structure and growth of oak species using Nelder trials along an environmental gradient: experimental design, evaluation methods, and results

    Directory of Open Access Journals (Sweden)

    Enno Uhl

    2015-05-01

    Full Text Available Background Most current approaches in forest science and practice require information about structure and growth of individual trees rather than - or in addition to - sum and mean values of growth and yield at forest stand level as provided by classic experimental designs. By inventing the wheel design, Nelder provided the possibility to turn to the individual tree as basic information unit. Such trials provide valuable insights into the dependency of growth on stand density at particular sites. Methods Here, we present an extension of the original design and evaluation by Nelder. (i We established Nelder wheels along an environmental gradient through Europe in atlantic climate in Belgium and Germany, Mediterranean climate in Italy, continental climate in Hungary as well as on high land climate in Mexico. Such disjunct Nelder wheels along an environmental gradient can be regarded and analysed as a two-factor design with the factors of site condition and stand density. (ii We present an advanced statistical approach to evaluate density dependent growth dynamics of trees planted in form of the Nelder design, which considers spatio-temporal autocorrelation. (iii We prove the usefulness of the methods in improving ecological theory concerning density related productivity, trade-offs between facilitation and competition, and allometric relations between size variables. Results First evaluations based on remeasured Nelder wheels in oak (Quercus roburL. show a size growth differentiation during the first observation period. In particular, height growth is accelerated under higher competition indicating facilitation effects. We detect furthermore a high variability in allometric relations. Conclusions The proposed design, methods, and results are discussed regarding their impact on forest practice, model building, and ecological theory. We conclude that the extended Nelder approach is highly efficient in providing currently lacking individual tree level

  2. The urban land price and its regional differences in China based on density gradient%基于密度梯度曲线的中国城市地价特征及区域差异

    Institute of Scientific and Technical Information of China (English)

    杨奎奇; 汪应宏; 张绍良; 赵清

    2012-01-01

    选取超大型综合性城市以外的全国不同区域和职能类型的33个城市为样本,采用地价密度梯度曲线模型,对其进行曲线回归分析,得出各城市商业、住宅地价的中心值和曲率系数。分析发现现阶段我国城市地价密度梯度曲线总体上符合城市地价变动的一般规律:城市中心地价与城市规模呈正相关关系;曲率系数与城市规模呈负相关关系;商业地价曲线曲率系数比住宅地价曲线曲率系数高。再此基础上对地价梯度曲线曲率系数进行聚类分析,总结出中国城市地价的不同类型特征。另外通过对我国东中西部30个城市样本的地价梯度系数的统计分析,从空间差异角度发现三类地区的地价呈现不同特征规律。%Taking 33 cities from different regions and categories in China as research ob- jects, this essay carries out land price curve regression analysis to simulate their land price density gradient. Then some coefficients of density gradient curve of urban commercial and residential land price are obtained. According to these coefficients, the phenomenon of Chinese urban land price density curves adapting to the normal rules of market economic countries is observed through analysis, which indicates that the city land price can reflect the true value of land, the main method of allocation of land resource in China through 30 years of marketing reform. Moreover, the conclusion that land price density gradients have different categories in China has been drawn based on clustering analysis of commer- cial and residential curve curvature. According to the commercial land price curve curva- ture and residential land price curvature, the cities in China except multi-center cities such as Beijing and Shanghai, are identified into four types. Every type of city is corresponding separately to different levels of development, city capacity, economic scale and parameters of land price curvature

  3. Speciation of lead in seawater and river water by using Saccharomyces cerevisiae immobilized in agarose gel as a binding agent in the diffusive gradients in thin films technique

    Energy Technology Data Exchange (ETDEWEB)

    Pescim, Guilherme Favoreto; Marrach, Gabriela; Vannuci-Silva, Monizze; Souza, Lais Alves; Menegario, Amauri Antonio [Universidade Estadual Paulista, Centro de Estudos Ambientais, Rio Claro, SP (Brazil)

    2012-09-15

    Saccharomyces cerevisiae immobilized in agarose gel is proposed as a binding agent for the diffusive gradients in thin films (DGT) technique for determination of Pb in river water and seawater. DGT samplers were assembled with the proposed binding agent (25-mm disk containing 20 %, m/v, S. cerevisiae and 3.0 %, m/v, agarose) and a diffusive layer of cellulose (3MM Chr chromatography paper of 25-mm diameter). The effects of some DGT parameters (e.g., immersion time, ionic strength, and pH) were evaluated. Elution of Pb from the binding agent was effectively done with 1.75 mol L{sup -1} HNO{sub 3}. The deployment curve (between 2 and 24 h) was characterized by a significant uptake of Pb (346 ng Pb h{sup -1}) and good linear regression (R {sup 2} = 0.9757). The experimental results are in excellent agreement with the predicted theoretical curve for mass uptake. Consistent results were found for solutions with ionic strengths of 0.005 mol L{sup -1} or greater and within a pH range of 4.5-8.5. Interferences from Cu (20:1), Mn (20:1), Fe (20:1), Zn (20:1), Ca (250:1), and Mg (250:1) in Pb retention were negligible. Determination of Pb in spiked river water samples (from the Corumbatai and Piracicaba rivers) performed using the proposed device was in agreement with total dissolved Pb, whereas measurements in seawater suggest that of the various species of Pb present in the samples, only cationic Pb species are adsorbed by the agarose-yeast gel disks. The in situ concentration of Pb obtained at two different sites of the Rio Claro stream (Corumbatai basin) were 1.13 {+-} 0.01 and 1.34 {+-} 0.04 {mu}g L{sup -1}. For 72-h deployments, a detection limit of 0.75 {mu}g L{sup -1} was calculated. The combination of inductively coupled plasma optical emission spectroscopy and in situ deployments of DGT samplers during the 72-h period makes possible the determination of labile Pb in river water. (orig.)

  4. Determination of mercury species by the diffusive gradient in thin film technique and liquid chromatography – atomic fluorescence spectrometry after microwave extraction

    Energy Technology Data Exchange (ETDEWEB)

    Pelcová, Pavlína, E-mail: pavlina.pelcova@mendelu.cz; Dočekalová, Hana, E-mail: hana.docekalova@mendelu.cz; Kleckerová, Andrea, E-mail: andrea.kleckerova@mendelu.cz

    2015-03-25

    Highlights: • DGT–MAE–LC–CV-AFS method was developed for determination of four mercury species. • The microwave extraction was used for isolation of mercury species from resin gels. • Optimized DGT–MAE–LC–CV-AFS method provides low detection limits (13–38 ng L{sup −1}). • The diffusion coefficients of four mercury species were simultaneously determined. - Abstract: A diffusive gradient in thin films technique (DGT) was combined with liquid chromatography (LC) and cold vapor atomic fluorescence spectrometry (CV-AFS) for the simultaneous quantification of four mercury species (Hg{sup 2+}, CH{sub 3}Hg{sup +}, C{sub 2}H{sub 5}Hg{sup +}, and C{sub 6}H{sub 5}Hg{sup +}). After diffusion through an agarose diffusive layer, the mercury species were accumulated in resin gels containing thiol-functionalized ion-exchange resins (Duolite GT73, and Ambersep GT74). A microwave-assisted extraction (MAE) in the presence of 6 M HCl and 5 M HCl (55 °C, 15 min) was used for isolation of mercury species from Ambersep and Duolite resin gels, respectively. The extraction efficiency was higher than 95.0% (RSD 3.5%). The mercury species were separated with a mobile phase containing 6.2% methanol + 0.05% 2-mercaptoethanol + 0.02 M ammonium acetate with a stepwise increase of methanol content up to 80% in the 16th min on a Zorbax C18 reverse phase column. The LODs of DGT–MAE–LC–CV-AFS method were 38 ng L{sup −1} for CH{sub 3}Hg{sup +}, 13 ng L{sup −1} for Hg{sup 2+}, 34 ng L{sup −1} for C{sub 2}H{sub 5}Hg{sup +} and 30 ng L{sup −1} for C{sub 6}H{sub 5}Hg{sup +} for 24 h DGT accumulation at 25 °C.

  5. Structure, spectra and antioxidant action of ascorbic acid studied by density functional theory, Raman spectroscopic and nuclear magnetic resonance techniques.

    Science.gov (United States)

    Singh, Gurpreet; Mohanty, B P; Saini, G S S

    2016-02-15

    Structure, vibrational and nuclear magnetic resonance spectra, and antioxidant action of ascorbic acid towards hydroxyl radicals have been studied computationally and in vitro by ultraviolet-visible, nuclear magnetic resonance and vibrational spectroscopic techniques. Time dependant density functional theory calculations have been employed to specify various electronic transitions in ultraviolet-visible spectra. Observed chemical shifts and vibrational bands in nuclear magnetic resonance and vibrational spectra, respectively have been assigned with the help of calculations. Changes in the structure of ascorbic acid in aqueous phase have been examined computationally and experimentally by recording Raman spectra in aqueous medium. Theoretical calculations of the interaction between ascorbic acid molecule and hydroxyl radical predicted the formation of dehydroascorbic acid as first product, which has been confirmed by comparing its simulated spectra with the corresponding spectra of ascorbic acid in presence of hydrogen peroxide. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. A pseudo-discrete algebraic reconstruction technique (PDART) prior image-based suppression of high density artifacts in computed tomography

    Science.gov (United States)

    Pua, Rizza; Park, Miran; Wi, Sunhee; Cho, Seungryong

    2016-12-01

    We propose a hybrid metal artifact reduction (MAR) approach for computed tomography (CT) that is computationally more efficient than a fully iterative reconstruction method, but at the same time achieves superior image quality to the interpolation-based in-painting techniques. Our proposed MAR method, an image-based artifact subtraction approach, utilizes an intermediate prior image reconstructed via PDART to recover the background information underlying the high density objects. For comparison, prior images generated by total-variation minimization (TVM) algorithm, as a realization of fully iterative approach, were also utilized as intermediate images. From the simulation and real experimental results, it has been shown that PDART drastically accelerates the reconstruction to an acceptable quality of prior images. Incorporating PDART-reconstructed prior images in the proposed MAR scheme achieved higher quality images than those by a conventional in-painting method. Furthermore, the results were comparable to the fully iterative MAR that uses high-quality TVM prior images.

  7. Structure, spectra and antioxidant action of ascorbic acid studied by density functional theory, Raman spectroscopic and nuclear magnetic resonance techniques

    Science.gov (United States)

    Singh, Gurpreet; Mohanty, B. P.; Saini, G. S. S.

    2016-02-01

    Structure, vibrational and nuclear magnetic resonance spectra, and antioxidant action of ascorbic acid towards hydroxyl radicals have been studied computationally and in vitro by ultraviolet-visible, nuclear magnetic resonance and vibrational spectroscopic techniques. Time dependant density functional theory calculations have been employed to specify various electronic transitions in ultraviolet-visible spectra. Observed chemical shifts and vibrational bands in nuclear magnetic resonance and vibrational spectra, respectively have been assigned with the help of calculations. Changes in the structure of ascorbic acid in aqueous phase have been examined computationally and experimentally by recording Raman spectra in aqueous medium. Theoretical calculations of the interaction between ascorbic acid molecule and hydroxyl radical predicted the formation of dehydroascorbic acid as first product, which has been confirmed by comparing its simulated spectra with the corresponding spectra of ascorbic acid in presence of hydrogen peroxide.

  8. Density functional theory investigation of hydrogen bonding effects on the oxygen, nitrogen and hydrogen electric field gradient and chemical shielding tensors of anhydrous chitosan crystalline structure.

    Science.gov (United States)

    Esrafili, Mehdi D; Elmi, Fatemeh; Hadipour, Nasser L

    2007-02-08

    A systematic computational investigation was carried out to characterize the 17O, 14N and 2H electric field gradient, EFG, as well as 17O, 15N, 13C and 1H chemical shielding tensors in the anhydrous chitosan crystalline structure. To include the hydrogen-bonding effects in the calculations, the most probable interacting molecules with the target molecule in the crystalline phase were considered through a hexameric cluster. The computations were performed with the B3LYP method and 6-311++G(d,p) and 6-31++G(d,p) standard basis sets using the Gaussian 98 suite of programs. Calculated EFG and chemical shielding tensors were used to evaluate the 17O, 14N and 2H nuclear quadrupole resonance, NQR, and 17O, 15N, 13C and 1H nuclear magnetic resonance, NMR, parameters in the hexameric cluster, which are in good agreement with the available experimental data. The difference between the calculated NQR and NMR parameters of the monomer and hexamer cluster shows how much hydrogen bonding interactions affect the EFG and chemical shielding tensors of each nucleus. These results indicate that both O(3)-H(33)...O(5-3) and N-H(22)...O(6-4) hydrogen bonding have a major influence on NQR and NMR parameters. Also, the quantum chemical calculations indicate that the intra- and intermolecular hydrogen bonding interactions play an essential role in determining the relative orientation of EFG and chemical shielding principal components in the molecular frame axes.

  9. Pancreatic islet isolation by mechanical-enzymatic separation, stationary collagenase digestion and dextran discontinuous density gradient purification: experimental study in dogs Isolamento das ilhotas pancreáticas pela separação mecânica-enzimática digestão estacionária com colagenase e purificação com gradiente de densidade descontínua de dextran: estudo experimental em cães

    Directory of Open Access Journals (Sweden)

    Jaques Waisberg

    2002-04-01

    Full Text Available The prospects for allotransplantation of pancreatic islets in man depend on the development of methods that provide sufficient quantities of pancreatic islets from a single donor, which are capable, when transplanted, of achieve the normalization of carbohydrate metabolism. Objective: Evaluate the efficacy of the isolation of Langerhans islets from dogs, by means of mechanical-enzymatic separation technique with stationary digestion using collagenase, and purification with a discontinuous dextran density gradient. Methods: The counting of islet numbers and evaluation of their sizes was accomplished by staining with diphenylthiocarbazone and using stereoscopic microscopes equipped with eyepiece reticule for the measurement of average diameters of stained islets. Results: The results disclosed that the average number of islets isolated was 81032.20 ± 24736.79 and the average number of islets isolated per kg of body weight was 6938.70 ± 1392.43. The average number of islets isolated per kg of body weight showed significant correlation with body weight and weight of the pancreas resected. Conclusion: The number of islets isolated, of a single donor, by mechanical-enzymatic separation, stationary collagenase digestion and discontinuous dextran density gradient purification can be sufficient to success of pancreatic islets transplant in dogs.A perspectiva do alotransplante de ilhotas pancreáticas no homem está na dependência do desenvolvimento de métodos que propiciem quantidades suficientes de ilhotas pancreáticas, originadas de doador único, capazes de, quando transplantadas, levarem à normalização do metabolismo dos hidratos de carbono. Objetivo: Avaliar, em cães, a eficácia do isolamento das ilhotas de Langerhans por meio da técnica de separação mecânica-enzimática, digestão estacionária com colagenase e purificação pelo gradiente de densidade descontínua de dextran. Métodos: A contagem do número e avaliação do tamanho

  10. Keutuhan Membran Spermatozoa Disekuensing Sentrifugasi Gradien Densitas Percoll Berpengencer Andromed dan CEP-2 yang Ditambahkan Kuning Telur (MEMBRANE INTACT OF SPERMATOZOA FOLLOWING SEXING USING PERCOLL DENSITY GRADIENT CENTRIFUGATION IN ANDROMED AND C

    Directory of Open Access Journals (Sweden)

    Yudha Fika Diliyana

    2014-05-01

    Full Text Available The aim of this study was to observe the best extender in protecting the membrane of bovine spermatozoafollowing sexing by percoll density gradient centrifugation. Freshly collected semen were obtained fromBalai Besar Inseminasi Buatan Singosari-Malang. The semen were diluted in andromed and CaudalEpididymal Plasma-2 (CEP-2 added with 10% egg yolk extenders.The sperm membrane integrity wasobserved using Hypo-osmotic Swelling Test (HOST. Sperm capacitation and acrososome reaction wereassessed using Chlortetracycline Fluorescence Assay.The results showed that andromed and CEP-2 addedwith 10% egg yolk were able to retain the sperm membrane integrity, whereas sperm capacitation andacrosome reaction were kept low. Caudal Epididymal Plasma-2 (CEP-2 added with 10% egg yolk seemedto give better protection towards the sperm membrane intact in comparison to andromed extender.

  11. In-phase and out-of-phase gradient-echo imaging in abdominal studies: intra-individual comparison of three different techniques

    Energy Technology Data Exchange (ETDEWEB)

    Ramalho, Miguel; Heredia, Vasco; Campos, Rafael O. P. de; Azevedo, Rafael M.; Semelka, Richard C. (Dept. of Radiology, Univ. of North Carolina at Chapel Hill (United States)); Dale, Brian M. (Siemens Medical Systems, Morrisville (United States)), email: richsem@med.unc.edu

    2012-05-15

    Background: T1-weighted gradient-echo in-phase and out-of-phase imaging is an essential component of comprehensive abdominal MR exams. It is useful for the study of fat-containing lesions and to identify various disease states related to the presence of fat in the liver. Purpose: To compare three T1-weighted in-phase and out-of-phase (IP/OP) gradient-echo imaging sequences in an intra-individual fashion, and to determine whether advantages exist for each of these sequences for various patient types. Material and Methods: One hundred and eighteen consecutive subjects (74 men, 44 women; mean age 53.9 +- 13.8 years) who had MRI examinations containing all three different IP/OP sequences (two-dimensional spoiled gradient-echo [2D-GRE], three-dimensional gradient-echo [3D-GRE], and magnetization-prepared gradient-recall echo [MP-GRE]) were included. Two different reviewers independently and blindly qualitatively evaluated IP/OP sequences to determine image quality, extent of artifacts, lesion detectability and conspicuity, and subjective grading of liver steatosis for the various sequences. Quantitative analysis was also performed. Qualitative and quantitative data were subjected to statistical analysis. Results: Respiratory ghosting, parallel imaging, and truncation artifacts as well as shading and blurring were more pronounced with 3D-GRE IP/OP imaging. Overall image quality was higher with 2D-GRE (P < 0.05). Detectability of low-fluid content lesions was lower with IP/OP MP-GRE sequences. MP-GRE sequences had the lowest SNRs (P < 0.001). Liver-to-spleen and liver-to-lesion CNRs were significantly lower with 3D-GRE and MP-GR, respectively (P < 0.001). Fat liver indexes showed strongly positive correlation between all sequences. Conclusion: Currently, 2D-GRE remains the best approach for clinical IP/OP imaging. The good image quality of MP-GRE sequences acquired in a free-breathing manner should recommend its use in patients unable to suspend breathing

  12. Using soft computing techniques to predict corrected air permeability using Thomeer parameters, air porosity and grain density

    Science.gov (United States)

    Nooruddin, Hasan A.; Anifowose, Fatai; Abdulraheem, Abdulazeez

    2014-03-01

    Soft computing techniques are recently becoming very popular in the oil industry. A number of computational intelligence-based predictive methods have been widely applied in the industry with high prediction capabilities. Some of the popular methods include feed-forward neural networks, radial basis function network, generalized regression neural network, functional networks, support vector regression and adaptive network fuzzy inference system. A comparative study among most popular soft computing techniques is presented using a large dataset published in literature describing multimodal pore systems in the Arab D formation. The inputs to the models are air porosity, grain density, and Thomeer parameters obtained using mercury injection capillary pressure profiles. Corrected air permeability is the target variable. Applying developed permeability models in recent reservoir characterization workflow ensures consistency between micro and macro scale information represented mainly by Thomeer parameters and absolute permeability. The dataset was divided into two parts with 80% of data used for training and 20% for testing. The target permeability variable was transformed to the logarithmic scale as a pre-processing step and to show better correlations with the input variables. Statistical and graphical analysis of the results including permeability cross-plots and detailed error measures were created. In general, the comparative study showed very close results among the developed models. The feed-forward neural network permeability model showed the lowest average relative error, average absolute relative error, standard deviations of error and root means squares making it the best model for such problems. Adaptive network fuzzy inference system also showed very good results.

  13. Difference between bulk and thin film densities of metal oxide and fluoride films studied by NRA depth profiling techniques

    CERN Document Server

    Coban, A; Durrani, S A

    2002-01-01

    Nuclear reaction analysis techniques have been used to study the difference between bulk and thin film densities of different dielectric (WO sub 3 , MgF sub 2 , NdF sub 3 , LaF sub 3 and ThF sub 4) thin films. Thicknesses of the films were measured by optical methods. The sup 1 sup 8 O(p,alpha) sup 1 sup 5 N reaction was used at 730 keV to profile WO sub 3 prepared with different thicknesses on a tantalum backing by thermal evaporation of natural WO sub 3. We have also successfully tested the sup 1 sup 8 O(p,alpha) sup 1 sup 5 N reaction at the 629 keV (GAMMA=2.1 keV) resonance for the same purpose. Excitation function measurement of the reaction was performed around the resonant energy at a detection angle of 150 deg. . In order to obtain the oxygen profiles of the thin films non-resonant part of the excitation function was deconvoluted using the known cross-section data of the reaction. Also, we studied different films of MgF sub 2 , NdF sub 3 , LaF sub 3 and ThF sub 4 using the 483.85 keV resonance in the ...

  14. Moving least squares-based multi-functional sensing technique or estimating viscosity and density of ternary solution

    Institute of Scientific and Technical Information of China (English)

    LIU Dan; WEI Guo; SUN Jin-wei; LIU Xin

    2009-01-01

    In the osmotic dehydration process of food, on-line estimation of concentrations of two components in ternary solution with NaCI and sucrose was performed based on multi-functional sensing technique.Moving Least Squares were adopted in approximation procedure to estimate the viscosity of such interested ternary solu-tion with the given data set.As a result, in one mode of using total experimental data as calibration data andvalidation data, the relative deviations of estimated viscosities are less than ~ 1.24%.In the other mode, by taking total experimental data except the ones for estimation as calibration data, the relative deviations are less than±3.47%.In the same way, the density of ternary solution can be also estimated with deviations less than ± 0.11% and ± 0.30% respectively in these two models.The satisfactory and accurate results show the ex-traordinary efficiency of Moving Least Squares behaved in signal approximation for multi-functional sensors.

  15. Strain-driven diffusion process during silicon oxidation investigated by coupling density functional theory and activation relaxation technique

    Science.gov (United States)

    Salles, N.; Richard, N.; Mousseau, N.; Hemeryck, A.

    2017-08-01

    The reaction of oxygen molecules on an oxidized silicon model-substrate is investigated using an efficient potential energy hypersurface exploration that provides a rich picture of the associated energy landscape, energy barriers, and insertion mechanisms. Oxygen molecules are brought in, one by one, onto an oxidized silicon substrate, and accurate pathways for sublayer oxidation are identified through the coupling of density functional theory to the activation relaxation technique nouveau, an open-ended unbiased reaction pathway searching method, allowing full exploration of potential energy surface. We show that strain energy increases with O coverage, driving the kinetics of diffusion at the Si/SiO2 interface in the interfacial layer and deeper into the bulk: at low coverage, interface reconstruction dominates while at high coverage, oxygen diffusion at the interface or even deeper into the bottom layers is favored. A changing trend in energetics is observed that favors atomic diffusions to occur at high coverage while they appear to be unlikely at low coverage. Upon increasing coverage, strain is accumulated at the interface, allowing the oxygen atom to diffuse as the strain becomes large enough. The observed atomic diffusion at the interface releases the accumulated strain, which is consistent with a layer-by-layer oxidation growth.

  16. Fabrication of Graded Density SiO2 Aerogel via Sol-Co-Gelation Techniques%溶胶共凝法制备准连续密度梯度SiO2气凝胶

    Institute of Scientific and Technical Information of China (English)

    归佳寅; 周斌; 钟艳红; 杜艾; 沈军

    2011-01-01

    针对气凝胶在航空航天领域的应用需求,为了进一步拓展单一密度气凝胶的应用范围,对密度梯度SiO2气胶的制备进行了研究.采用自建密度梯度制备成型装置,以正硅酸乙酯为有机硅源,经溶胶共凝工艺,结合CO2超临干燥技术,获得密度范围在60~160 mg/cm3的准连续密度梯度SiO2气凝胶.采用光学显微镜、扫描电子显微镜、X线相衬成像等手段对制备获得的SiO2气凝胶进行测试表征.通过与逐层凝胶法制备的密度渐变SiO2气凝胶进行列发现,溶胶共凝工艺消除了层间界面处所产生的致密层和密度突变,有利于相邻两层间的互扩散,使密度变化更趋于续.采用该法制备的密度梯度气凝胶拓展了常规气凝胶的应用范围,在声阻抗匹配和空间粒子捕获等领域有潜在的用前景.%For the application requirement in aerospace and further expanding the application field of aerogel, the fabrication of gradient density aerogel SiO2 has been researched in this paper. By a self-built device, a continuously graded silica aerogel is fabricated via special sol-co-gelation techniques, with tetraethoxysilane (TEOS) as the organic precursor and by a supercritical drying process. Its density ranges from 60 mg/cm3 to 160 mg/cm3. Scan electron microscope, X-ray phase contrast method and optical microscopy are used to characterize the graded density silica aerogel prepared. Compared with the graded density silica aerogel produced by layer-by-layer gelation techniques, the sol-co-gelation techniques smooth out density mutation and increase the interdiffusion between adjacent layers, which makes density change tend to be more continuous. The gradient density aerogel fabricated by such a method expands greatly the application of conditional aerogel. It can potentially be used in many fields such as acoustic impedance matching and interstellar particles collection.

  17. Utilization of combined remote sensing techniques to detect environmental variables influencing malaria vector densities in rural West Africa

    Science.gov (United States)

    2012-01-01

    Introduction The use of remote sensing has found its way into the field of epidemiology within the last decades. With the increased sensor resolution of recent and future satellites new possibilities emerge for high resolution risk modeling and risk mapping. Methods A SPOT 5 satellite image, taken during the rainy season 2009 was used for calculating indices by combining the image's spectral bands. Besides the widely used Normalized Difference Vegetation Index (NDVI) other indices were tested for significant correlation against field observations. Multiple steps, including the detection of surface water, its breeding appropriateness for Anopheles and modeling of vector imagines abundance, were performed. Data collection on larvae, adult vectors and geographic parameters in the field, was amended by using remote sensing techniques to gather data on altitude (Digital Elevation Model = DEM), precipitation (Tropical Rainfall Measurement Mission = TRMM), land surface temperatures (LST). Results The DEM derived altitude as well as indices calculations combining the satellite's spectral bands (NDTI = Normalized Difference Turbidity Index, NDWI Mac Feeters = Normalized Difference Water Index) turned out to be reliable indicators for surface water in the local geographic setting. While Anopheles larvae abundance in habitats is driven by multiple, interconnected factors - amongst which the NDVI - and precipitation events, the presence of vector imagines was found to be correlated negatively to remotely sensed LST and positively to the cumulated amount of rainfall in the preceding 15 days and to the Normalized Difference Pond Index (NDPI) within the 500 m buffer zone around capture points. Conclusions Remotely sensed geographical and meteorological factors, including precipitations, temperature, as well as vegetation, humidity and land cover indicators could be used as explanatory variables for surface water presence, larval development and imagines densities. This modeling

  18. Utilization of combined remote sensing techniques to detect environmental variables influencing malaria vector densities in rural West Africa.

    Science.gov (United States)

    Dambach, Peter; Machault, Vanessa; Lacaux, Jean-Pierre; Vignolles, Cécile; Sié, Ali; Sauerborn, Rainer

    2012-03-23

    The use of remote sensing has found its way into the field of epidemiology within the last decades. With the increased sensor resolution of recent and future satellites new possibilities emerge for high resolution risk modeling and risk mapping. A SPOT 5 satellite image, taken during the rainy season 2009 was used for calculating indices by combining the image's spectral bands. Besides the widely used Normalized Difference Vegetation Index (NDVI) other indices were tested for significant correlation against field observations. Multiple steps, including the detection of surface water, its breeding appropriateness for Anopheles and modeling of vector imagines abundance, were performed. Data collection on larvae, adult vectors and geographic parameters in the field, was amended by using remote sensing techniques to gather data on altitude (Digital Elevation Model = DEM), precipitation (Tropical Rainfall Measurement Mission = TRMM), land surface temperatures (LST). The DEM derived altitude as well as indices calculations combining the satellite's spectral bands (NDTI = Normalized Difference Turbidity Index, NDWI Mac Feeters = Normalized Difference Water Index) turned out to be reliable indicators for surface water in the local geographic setting. While Anopheles larvae abundance in habitats is driven by multiple, interconnected factors - amongst which the NDVI - and precipitation events, the presence of vector imagines was found to be correlated negatively to remotely sensed LST and positively to the cumulated amount of rainfall in the preceding 15 days and to the Normalized Difference Pond Index (NDPI) within the 500 m buffer zone around capture points. Remotely sensed geographical and meteorological factors, including precipitations, temperature, as well as vegetation, humidity and land cover indicators could be used as explanatory variables for surface water presence, larval development and imagines densities. This modeling approach based on remotely sensed

  19. Utilization of combined remote sensing techniques to detect environmental variables influencing malaria vector densities in rural West Africa

    Directory of Open Access Journals (Sweden)

    Dambach Peter

    2012-03-01

    Full Text Available Abstract Introduction The use of remote sensing has found its way into the field of epidemiology within the last decades. With the increased sensor resolution of recent and future satellites new possibilities emerge for high resolution risk modeling and risk mapping. Methods A SPOT 5 satellite image, taken during the rainy season 2009 was used for calculating indices by combining the image's spectral bands. Besides the widely used Normalized Difference Vegetation Index (NDVI other indices were tested for significant correlation against field observations. Multiple steps, including the detection of surface water, its breeding appropriateness for Anopheles and modeling of vector imagines abundance, were performed. Data collection on larvae, adult vectors and geographic parameters in the field, was amended by using remote sensing techniques to gather data on altitude (Digital Elevation Model = DEM, precipitation (Tropical Rainfall Measurement Mission = TRMM, land surface temperatures (LST. Results The DEM derived altitude as well as indices calculations combining the satellite's spectral bands (NDTI = Normalized Difference Turbidity Index, NDWI Mac Feeters = Normalized Difference Water Index turned out to be reliable indicators for surface water in the local geographic setting. While Anopheles larvae abundance in habitats is driven by multiple, interconnected factors - amongst which the NDVI - and precipitation events, the presence of vector imagines was found to be correlated negatively to remotely sensed LST and positively to the cumulated amount of rainfall in the preceding 15 days and to the Normalized Difference Pond Index (NDPI within the 500 m buffer zone around capture points. Conclusions Remotely sensed geographical and meteorological factors, including precipitations, temperature, as well as vegetation, humidity and land cover indicators could be used as explanatory variables for surface water presence, larval development and imagines

  20. Study of diffusion in cartilage by the ''PFG'' (pulsed-field-gradient) NMR technique; Studium der Diffusion im Knorpel mit der ''PFG'' (pulsed-field-gradient)-NMR-Technik

    Energy Technology Data Exchange (ETDEWEB)

    Naji, L.; Trampel, R.; Ngwa, W.; Knauss, R.; Schiller, J.; Arnold, K. [Leipzig Univ. (Germany). Inst. fuer Medizinische Physik und Biophysik

    2001-07-01

    Since cartilage does not contain any blood vessels, diffusion is the most important mechanism for its supply. Although several methods are available for the measurement of diffusion, this study focuses exclusively on NMR methods. Besides the ''classic'' water diffusion, the diffusion behaviour of ions and polymers in cartilage is also described. In all cases, and at short observation times, diffusion is mostly determined by the water content of the sample. However, the variation of the observation time allows to obtain information also on the internal structure of cartilage. In addition, it is discussed to which extent the individual techniques allow conclusions with respect to degenerative joint diseases, and under which in vivo conditions they can be applied. (orig.) [German] Das Fehlen von Blutgefaessen im Knorpel macht die Diffusion zum wichtigsten Transportmechanismus fuer die Versorgung des Knorpels. Obwohl unterschiedliche Methoden zur Messung von Diffusionsprozessen eingesetzt werden koennen, beschraenken wir uns hie ausschliesslich auf NMR-Methoden. Neben der ''klassischen'' Wasserdiffusion werden auch die Diffusion von Ionen und die Polymerdiffusion in Knorpel beschrieben. In allen Faellen gilt, dass ueber kurze Beobachtungszeiten das Diffusionsverhalten im wesentlichen durch den Wassergehalt bestimmt wird. Jedoch werden durch Variation der Beobachtungszeit auch Informationen ueber die innere Struktur des Knorpels erhalten. Es wird ausserdem dargelegt, inwieweit Techniken Rueckschluesse auf degenerative Gelenkveraenderungen zugelassen und wie sie unter In-vivo-Bedingungen genutzt werden koennten. (orig.)

  1. A numerical study of scalar gradients in Kelvin-Helmholtz billows

    Science.gov (United States)

    Parker, J. W.; Bowhill, S. A.

    1989-01-01

    A high resolution numerical technique is used to model the development of a periodically perturbed shear layer imbedded in an initially vertical gradient of a passive scalar. The technique follows the development of the vorticity through an initial linear growth state and well into the nonlinear development of Kelvin-Helmholtz billows, in the zero-viscosity, zero-diffusion limit. The resulting scalar distribution rapidly develops regions of extremely sharp scalar gradients, which wind around the periodically spaced vortical low gradient cores. Vertical cross sections through different parts of the billow structure are presented and compared with rocket measurements of electron density fine structure in the mesosphere. Gradient limits imposed by finite diffusion are calculated, and implications for atmospheric radar observations are discussed.

  2. Thermally tailored gradient topography surface on elastomeric thin films.

    Science.gov (United States)

    Roy, Sudeshna; Bhandaru, Nandini; Das, Ritopa; Harikrishnan, G; Mukherjee, Rabibrata

    2014-05-14

    We report a simple method for creating a nanopatterned surface with continuous variation in feature height on an elastomeric thin film. The technique is based on imprinting the surface of a film of thermo-curable elastomer (Sylgard 184), which has continuous variation in cross-linking density introduced by means of differential heating. This results in variation of viscoelasticity across the length of the surface and the film exhibits differential partial relaxation after imprinting with a flexible stamp and subjecting it to an externally applied stress for a transient duration. An intrinsic perfect negative replica of the stamp pattern is initially created over the entire film surface as long as the external force remains active. After the external force is withdrawn, there is partial relaxation of the applied stresses, which is manifested as reduction in amplitude of the imprinted features. Due to the spatial viscoelasticity gradient, the extent of stress relaxation induced feature height reduction varies across the length of the film (L), resulting in a surface with a gradient topography with progressively varying feature heights (hF). The steepness of the gradient can be controlled by varying the temperature gradient as well as the duration of precuring of the film prior to imprinting. The method has also been utilized for fabricating wettability gradient surfaces using a high aspect ratio biomimetic stamp. The use of a flexible stamp allows the technique to be extended for creating a gradient topography on nonplanar surfaces as well. We also show that the gradient surfaces with regular structures can be used in combinatorial studies related to pattern directed dewetting.

  3. Técnicas de medida de densidad de masa ósea Bone mineral density measurement techniques

    Directory of Open Access Journals (Sweden)

    R. Ibáñez

    2003-01-01

    Full Text Available Los densitómetros por absorciometría fotónica simple y dual dejaron paso a las técnicas no isotópicas, absorciometría de rayos X simple y dual, que son las que más se usan en la actualidad. La técnica más extendida es la absorciometría de doble energía de rayos X, DXA, especialmente los densitómetros que analizan cadera y columna lumbar. Es una técnica precisa, fiable, y se ha demostrado que es capaz de predecir fracturas. Sin embargo, los densitómetros periféricos, tanto los que se basan en tecnología dexa como los de ultrasonidos, también han demostrado su utilidad. La topografía cuantificada computerizada es el único densitómetro tridimensional, capaz de diferenciar entre hueso cortical y trabecular. Tiene el inconveniente de la alta radiación y el alto coste económico. Repasamos las características, así como las ventajas e inconvenientes, de cada una de estas técnicas de medida de masa ósea. En el presente articulo se repasan las distintas técnicas densitométricas desarrolladas en los últimos años y los distintos criterios sobre las indicaciones de densitometría. No existen criterios unánimes al respecto, sin embargo distintos organismos y sociedades científicas han elaborado recomendaciones, basadas en los factores de riesgo de osteoporosis o de fractura, que pueden ser una guía útil para la práctica clínica.Different techniques for measuring bone mass density developed in the last years are revised. Single and dual fotonic absorciometry are now outdated by the use of single and dual x-ray absorciometry which don’t need an isotope source. The most widely used technique now is the dual energy x-ray asbsorciometry , specially the ones who analyse lumbar spine and hip. It is an accurate and precise tool and has proved to be a good predictor of fractures. Nevertherless, peripheral densitometers based on dual energy x-ray or on broadband ultrasonic attenuation, have also proved to be of utility

  4. Velocity Gradients as a Tracer for Magnetic Fields

    Science.gov (United States)

    González-Casanova, Diego F.; Lazarian, A.

    2017-01-01

    Strong Alfvénic turbulence develops eddy-like motions perpendicular to the local direction of magnetic fields. This local alignment induces velocity gradients perpendicular to the local direction of the magnetic field. We use this fact to propose a new technique of studying the direction of magnetic fields from observations, which we call the velocity gradient technique. We test our idea by employing the synthetic observations obtained via 3D magnetohydrodynamical (MHD) numerical simulations for different sonic and Alfvén Mach numbers. We calculate the velocity gradient, {\\boldsymbol{Ω }}, using the velocity centroids. We find that {\\boldsymbol{Ω }} traces the projected magnetic field best for the synthetic maps obtained with sub-Alfvénic simulations and provides good point-wise correspondence between the magnetic field direction and the direction of {\\boldsymbol{Ω }}. The reported alignment is much better than the alignment between the density gradients and the magnetic field, and we demonstrate that it can be used to find the magnetic field strength with an analog of the Chandrasekhar–Fermi method. This new technique does not require dust polarimetry, and our study opens up a new way of studying magnetic fields using spectroscopic data.

  5. PIV MEASUREMENTS FOR GAS FLOW UNDER GRADIENT MAGNETIC FIELDS

    Institute of Scientific and Technical Information of China (English)

    RUAN Xiaodong; WU Feng; F.YAMAMOTO

    2004-01-01

    Particle Image Velocimetry (PIV) techniques were developed to measure the convective N2-air flow under gradient magnetic fields. The velocity fields were calculated by the Minimum Quadratic Difference (MQD) algorithm and spurious vectors were eliminated by Delaunay Tessellation.The N2-air flow was measured as the magnetic flux density varying from 0 ~ 1.5 T. A strengthened vortex flow of air was observed under the condition that the magnetic field was applied, and the velocity of N2 jet rose with the increase of the magnetic density. The experimental results show that the magnetic force will induce a vortex flow and cause a convection flow of the air mixture when both gradients of the O2 concentration and the magnetic field intensity exist.

  6. CO{sub 2} laser diagnostics for measurements of the plasma density profile and plasma density fluctuations on LHD

    Energy Technology Data Exchange (ETDEWEB)

    Vyacheslavov, L.N. [Budker Institute of Nuclear Physics, Novosibirsk (Russian Federation); Tanaka, K.; Kawahata, K. [National Inst. for Fusion Science, Toki, Gifu (Japan)

    2001-04-01

    A CO{sub 2} laser based diagnostics complimentary to the existing FIR interferometer is proposed. It combines interferometry for plasma density profile measurement under conditions of large density gradients, and both imaging and scattering techniques for observation of plasma fluctuations. Two-colour interferometer with a slablike probe beam and a single multichannel linear detector array provide observation of plasma density profile and density fluctuations at two locations along the probe beam. Basic characteristics of the diagnostics are considered as well as some effects that include dispersion and are critical for two colour imaging technique. The results of the bench-top experiments with the prototype of the interferometer are presented. (author)

  7. An investigation of hydrogen-bonding effects on the nitrogen and hydrogen electric field gradient and chemical shielding tensors in the 9-methyladenine real crystalline structure: a density functional theory study.

    Science.gov (United States)

    Mirzaei, Mahmoud; Hadipour, Nasser L

    2006-04-13

    Hydrogen-bonding effects in the real crystalline structure of 9-methyladenine, 9-MA, were studied using calculated electric field gradient, EFG, and chemical shielding, CS, tensors for nitrogen and hydrogen nuclei via density functional theory. The calculations were carried out at the B3LYP and B3PW91 levels with the 6-311++G basis set via the Gaussian 98 package. Nuclear quadrupole coupling constants, C(Q), and asymmetry parameters, eta(Q), are reported for (14)N and (2)H. The chemical shielding anisotropy, Deltasigma, and chemical shielding isotropy, sigma(iso), are also reported for (15)N and (1)H. The difference between the calculated parameters of the monomer and heptameric layer-like cluster 9-MA shows how much H-bonding interactions affect the EFG and CS tensors of each nucleus. This result indicates that N(10) (imino nitrogen) has a major role in H-bonding interactions, whereas that of N(9) is negligible. There is good agreement between the present calculated parameters and reported experimental data. Although some discrepancies were observed, this could be attributed to the different conditions which were applied for calculation and the experiments.

  8. A Diffusive Gradient-in-Thin-Film Technique for Evaluation of the Bioavailability of Cd in Soil Contaminated with Cd and Pb

    Directory of Open Access Journals (Sweden)

    Peifang Wang

    2016-06-01

    Full Text Available Management of heavy metal contamination requires accurate information about the distribution of bioavailable fractions, and about exchange between the solid and solution phases. In this study, we employed diffusive gradients in thin-films (DGT and traditional chemical extraction methods (soil solution, HOAc, EDTA, CaCl2, and NaOAc to determine the Cd bioavailability in Cd-contaminated soil with the addition of Pb. Two typical terrestrial species (wheat, Bainong AK58; maize, Zhengdan 958 were selected as the accumulation plants. The results showed that the added Pb may enhance the efficiency of Cd phytoextraction which is indicated by the increasing concentration of Cd accumulating in the plant tissues. The DGT-measured Cd concentrations and all the selected traditional extractants measured Cd concentrations all increased with increasing concentration of the addition Pb which were similar to the change trends of the accumulated Cd concentrations in plant tissues. Moreover, the Pearson regression coefficients between the different indicators obtained Cd concentrations and plants uptake Cd concentrations were further indicated significant correlations (p < 0.01. However, the values of Pearson regression coefficients showed the merits of DGT, CaCl2, and Csol over the other three methods. Consequently, the in situ measurement of DGT and the ex situ traditional methods could all reflect the inhibition effects between Cd and Pb. Due to the feature of dynamic measurements of DGT, it could be a robust tool to predict Cd bioavaiability in complex contaminated soil.

  9. A Diffusive Gradient-in-Thin-Film Technique for Evaluation of the Bioavailability of Cd in Soil Contaminated with Cd and Pb.

    Science.gov (United States)

    Wang, Peifang; Wang, Teng; Yao, Yu; Wang, Chao; Liu, Cui; Yuan, Ye

    2016-06-02

    Management of heavy metal contamination requires accurate information about the distribution of bioavailable fractions, and about exchange between the solid and solution phases. In this study, we employed diffusive gradients in thin-films (DGT) and traditional chemical extraction methods (soil solution, HOAc, EDTA, CaCl₂, and NaOAc) to determine the Cd bioavailability in Cd-contaminated soil with the addition of Pb. Two typical terrestrial species (wheat, Bainong AK58; maize, Zhengdan 958) were selected as the accumulation plants. The results showed that the added Pb may enhance the efficiency of Cd phytoextraction which is indicated by the increasing concentration of Cd accumulating in the plant tissues. The DGT-measured Cd concentrations and all the selected traditional extractants measured Cd concentrations all increased with increasing concentration of the addition Pb which were similar to the change trends of the accumulated Cd concentrations in plant tissues. Moreover, the Pearson regression coefficients between the different indicators obtained Cd concentrations and plants uptake Cd concentrations were further indicated significant correlations (p < 0.01). However, the values of Pearson regression coefficients showed the merits of DGT, CaCl₂, and Csol over the other three methods. Consequently, the in situ measurement of DGT and the ex situ traditional methods could all reflect the inhibition effects between Cd and Pb. Due to the feature of dynamic measurements of DGT, it could be a robust tool to predict Cd bioavaiability in complex contaminated soil.

  10. Selective sampling and measurement of Cr (VI) in water with polyquaternary ammonium salt as a binding agent in diffusive gradients in thin-films technique.

    Science.gov (United States)

    Chen, Hong; Zhang, Yang-Yang; Zhong, Ke-Li; Guo, Lian-Wen; Gu, Jia-Li; Bo, Le; Zhang, Meng-Han; Li, Jian-Rong

    2014-04-30

    A diffusive gradients in thin films (DGT) device with polyquaternary ammonium salt (PQAS) as a novel binding agent (PQAS DGT) combined with graphite furnace atomic absorption spectrometry (GFAAS) was developed for the selective sampling and measurement of Cr (VI) in water. The performance of PQAS DGT was independent of pH 3-12 and ionic strength from 1 × 10(-3) to 1 molL(-1). DGT validation experiments showed that Cr (VI) was measured accurately as well as selectively by PQAS DGT, whereas Cr (III) was not determined quantitatively. Compared with diphenylcarbazide spectrophotometric method (DPC), the measurement of Cr (VI) with PQAS DGT was agreement with that of DPC method in the industrial wastewater. PQAS-DGT device had been successfully deployed in local freshwater. The concentrations of Cr (VI) determined by PQAS DGT coupled with GFAAS in Nuer River, Ling River and North Lake were 0.73 ± 0.09 μg L(-1), 0.50 ± 0.07 μg L(-1) and 0.61 ± 0.07 μg L(-1), respectively. The results indicate that PQAS DGT device can be used for the selective sampling and measurement Cr (VI) in water and its detection limit is lower than that of DPC method. Copyright © 2014 Elsevier B.V. All rights reserved.

  11. Isolation and structure elucidation of a highly haemolytic saponin from the Merck saponin extract using high-field gradient-enhanced NMR techniques.

    Science.gov (United States)

    Delay, C; Gavin, J A; Aumelas, A; Bonnet, P A; Roumestand, C

    1997-07-11

    Saponins SAPO50 and SAPO30, of which SAPO50 is highly haemolytic, have been isolated from the commercial Merck Saponin. Their structures have been determined exclusively by high-field gradient-enhanced NMR methods. The 1H and 13C NMR spectra of these saponins in pyridine-deuterium oxide have been assigned by homonuclear and heteronuclear correlation experiments. Anomeric configurations were obtained by combined use of 1JCH, 3JH-1.H-2, and 1D-NOESY data. Sugar residues were identified by use of 3JHH values obtained from their subspectra recorded using an optimized 1D-zeta-TOCSY sequence. Linkage assignments were made using the ge-HMBC and 1D-NOESY spectra. This study shows that SAPO50 represents a hitherto undescribed saponin with the following structure: 3-O-beta-D-xylopyranosyl-(1-->3)-[beta-D-galactopyranosyl- (1-->2)]-beta-D-glucuronopyranosyl gypsogenin 28-O-(6-deoxy-beta-D-glucopyranosyl)-(1-->4)-[beta-D-xylopyranosyl-(1--> 3)- beta-D-xylopyranosyl-(1-->4)]-alpha-L-rhamnopyranosyl-(1-->2)-beta-D- fucopyranoside. SAPO30, however, corresponds to a saponin previously described [D. Frechet, B. Christ, B. Monegier du Sorbier, H. Fischer, and M. Vuilhorgne, Phytochemistry, 30 (1991) 927-931].

  12. BREAST DENSITY EVALUATION: A COMPARISON BETWEEN ASSESSMENT BY A RADIOLOGIST AND THE COMPUTER-ASSISTED THRESHOLD TECHNIQUE

    OpenAIRE

    2015-01-01

    Traditionally, mammographic density (MD) of the breast has been assessed by a radiologist visually. This subjective evaluation requires significant experience to distinguish the relative proportions of the fibrous connective tissue and adipose tissue in the mammary gland correctly. The aim of this study is to compare the capabilities of the different methods (visual and computer-assisted) for assessing mammographic density. Our sample in this study consists of 66 patients with digital m...

  13. Enriching the fetal nuclear red blood cells by intervening membrane ionic channels and density gradient centrifugation%干预离子通道联合密度梯度离心富集胎儿有核红细胞

    Institute of Scientific and Technical Information of China (English)

    刘芳; 白亚娜; 张丽娜; 徐向红; 程宁

    2011-01-01

    Objective To investigate the effectiveness of the Nucleated Red Blood Cells' enrichment by intervening membrance ionic channels-K + /Cl- cotransporter and density gradient centrifugation in umbilical cord blood. Methods Setting up optical Intervening condition of maximal change of cell volume from RBCs on umbilical cord blood, the harvest rate of cell enrichment by different density medium centrifugation were determined by flow cytometry. Results Compared to traditional enriching method, the average purity of NRBC enrichment in the umbilical cord blood could take from 8. 6% to 69. 9% by centrifugation of 1. 065 g/L medium and 55. 9% by 1. 099 g/L medium after intervening, which means to respectively increase enrichment of NRBC. Conclusion Both urea intervention and density centrifugation may effectively promote the harvest rate of the NRBC enrichment.%目的 探讨干预膜表面K-CI同向转运体(KCC)离子通道联合密度梯度离心对富集脐血中胎儿有核红细胞(NRBC)的效果.方法 用KCC特异激活剂尿素(Urea)干预细胞膜,建立最大限度缩小细胞体积的干预条件;用最佳干预条件干预脐血后进行不同密度介质下离心富集NRBC,用流式细胞仪计数NRBC的富集效果.结果 比较传统1.077淋巴细胞分离液离心后的单个核细胞层流式细胞仪计数NRBC为8.6%,干预脐血后用1.065密度介质离心后下层NRBC达69.9%,未干预组中用1.099密度介质离心的单个核细胞层NRBC达55.9%,提高了富集量.结论 尿素干预联合密度梯度离心能有效提高NRBC的富集率.

  14. Determination of photocarrier density under continuous photoirradiation using spectroscopic techniques as applied to polymer: Fullerene blend films

    Energy Technology Data Exchange (ETDEWEB)

    Kanemoto, Katsuichi, E-mail: kkane@sci.osaka-cu.ac.jp; Nakatani, Hitomi; Domoto, Shinya [Department of Physics, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585 (Japan)

    2014-10-28

    We propose a method to determine the density of photocarrier under continuous photoirradiation in conjugated polymers using spectroscopic signals obtained by photoinduced absorption (PIA) measurements. The bleaching signals in the PIA measurements of polymer films and the steady-state absorption signals of oxidized polymer solution are employed to determine the photocarrier density. The method is applied to photocarriers of poly (3-hexylthiophene) (P3HT) in a blended film consisting of P3HT and [6,6]-phenyl C61 butyric acid methyl ester (PCBM). The photocarrier density under continuous photoirradiation of 580 mW/cm{sup 2} is determined to be 3.5 × 10{sup 16 }cm{sup −3}. Using a trend of the carrier density increasing in proportion to the square root of photo-excitation intensity, we provide a general formula to estimate the photocarrier density under simulated 1 sun solar irradiation for the P3HT: PCBM film of an arbitrary thickness. We emphasize that the method proposed in this study enables an estimate of carrier density without measuring a current and can be applied to films with no electrodes as well as to devices.

  15. Suction/inspiration against resistance or standardized Mueller maneuver: a new breathing technique to improve contrast density within the pulmonary artery: a pilot CT study

    Energy Technology Data Exchange (ETDEWEB)

    Gutzeit, Andreas [Paracelsus Medical University Salzburg, Department of Radiology, Salzburg (Austria); Hirslanden Hospital St. Anna, Institute of Radiology and Nuclear Medicine, Clinical Research Unit, Lucerne (Switzerland); Kantonsspital Winterthur, Department of Radiology, Winterthur (Switzerland); Froehlich, Johannes M.; Weymarn, Constantin von; Goyen, Matthias [Hirslanden Hospital St. Anna, Institute of Radiology and Nuclear Medicine, Clinical Research Unit, Lucerne (Switzerland); Waelti, Stephan [Cantonal Hospital St. Gallen, Department of Radiology and Nuclear Medicine, St. Gallen (Switzerland); Roos, Justus E. [Duke University Medical Center, Department of Radiology, Durham, NC (United States); Meissnitzer, Matthias; Hergan, Klaus [Paracelsus Medical University Salzburg, Department of Radiology, Salzburg (Austria); Czell, David [Cantonal Hospital Winterthur, Department of Neurology, Winterthur (Switzerland); Reischauer, Carolin [Paracelsus Medical University Salzburg, Department of Radiology, Salzburg (Austria); Hirslanden Hospital St. Anna, Institute of Radiology and Nuclear Medicine, Clinical Research Unit, Lucerne (Switzerland)

    2015-11-15

    Our aim was to prospectively investigate whether the recently introduced suction/inspiration against resistance breathing method leads to higher computed tomography (CT) contrast density in the pulmonary artery compared to standard breathing. The present study was approved by the Medical Ethics committee and all subjects gave written informed consent. Fifteen patients, each without suspicious lung emboli, were randomly assigned to four different groups with different breathing maneuvers (suction against resistance, Valsalva, inspiration, expiration) during routine CT. Contrast enhancement in the central and peripheral sections of the pulmonary artery were measured and compared with one another. Peripheral enhancement during suction yielded increased mean densities of 138.14 Hounsfield units (HU) (p = 0.001), compared to Valsalva and a mean density of 67.97 HU superior to inspiration (p = 0.075). Finally, suction in comparison to expiration resulted in a mean increase of 30.51 HU (p = 0.42). Central parts of pulmonary arteries presented significantly increased enhancement values (95.74 HU) for suction versus the Valsalva technique (p = 0.020), while all other mean densities were in favour of suction (versus inspiration: p = 0.201; versus expiration: p = 0.790) without reaching significance. Suction/Inspiration against resistance is a promising technique to improve contrast density within pulmonary vessels, especially in the peripheral parts, in comparison to other breathing maneuvers. (orig.)

  16. Multi-parameter gradient procedure for polarimetry data inversion in tokamak plasma

    Energy Technology Data Exchange (ETDEWEB)

    Chrzanowski, J., E-mail: j.chrzanowski@am.szczecin.pl [Maritime University, Szczecin Wały Chrobrego 1/2 (Poland); Kravtsov, Yu. A. [Maritime University, Szczecin Wały Chrobrego 1/2 (Poland); Mazon, D. [Association Euratom/CEA, CEA Cadarache DSM/IRFM, 13108 St. Paul lez Durance Cedex (France); JET, Culham (United Kingdom)

    2013-10-15

    Highlights: ► We use gradient procedure to fit plasma parameters to polarimetric data. ► Calculations are performed in developed by authors angular variables technique. ► Numerical results are compared with experimentally measured angular parameters. ► We observe satisfied accuracy of inversion procedure after several iterations. -- Abstract: Multi-parameter gradient procedure is suggested which allows fitting tokamak plasma model to polarimetric data. One of the simplest version of gradient procedure deals with four parameters model: maximum values of electron density, maximum value of electric current density in plasma, common radius of electron density, electric current distributions and increment of the safety factor inside plasma. Using recently developed by authors angular variables technique (AVT) in plasma polarimetry we may compute angular parameters of polarization ellipse for a given set of four plasma parameters and compare them with experimentally measured angular parameters. With angular parameters, measured in two channels polarimetric system (two azimuthal and two ellipticity angles, totally four experimental values). Applying then gradient procedure for squared difference between computed and measured parameters, we find four parameters of plasma model and thereby perform inversion of polarimetric data. Numerical simulations have approved that gradient procedure provides acceptable accuracy of inversion already after several iterations.

  17. Selective sampling and measurement of Cr (VI) in water with polyquaternary ammonium salt as a binding agent in diffusive gradients in thin-films technique

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Hong, E-mail: redastar@163.com [College of Chemistry, Chemical Engineering and Food Safety, Bohai University (China); Research Institute of Food Science, Bohai University (China); Food Safety Key Lab of Liaoning Province, Jinzhou 121013 (China); Zhang, Yang-Yang; Zhong, Ke-Li; Guo, Lian-Wen [College of Chemistry, Chemical Engineering and Food Safety, Bohai University (China); Research Institute of Food Science, Bohai University (China); Food Safety Key Lab of Liaoning Province, Jinzhou 121013 (China); Gu, Jia-Li [College of Chemistry, Chemical Engineering and Food Safety, Bohai University (China); Bo, Le; Zhang, Meng-Han [College of Chemistry, Chemical Engineering and Food Safety, Bohai University (China); Research Institute of Food Science, Bohai University (China); Food Safety Key Lab of Liaoning Province, Jinzhou 121013 (China); Li, Jian-Rong, E-mail: lijianrong@zjgsu.edu.cn [College of Chemistry, Chemical Engineering and Food Safety, Bohai University (China); Research Institute of Food Science, Bohai University (China); Food Safety Key Lab of Liaoning Province, Jinzhou 121013 (China)

    2014-04-01

    Graphical abstract: - Highlights: • We develop a new DGT device for in situ sampling Cr (VI) in water. • Polyquaternary ammonium salt (PQAS) was used as binding agent of DGT device. • Cr (VI) can be accumulated in the PQAS binding phase whereas Cr (III) cannot. • The DGT performance was independent of pH 3–12 and ionic strength 1 × 10{sup −3}–1 mol L{sup −1}. - Abstract: A diffusive gradients in thin films (DGT) device with polyquaternary ammonium salt (PQAS) as a novel binding agent (PQAS DGT) combined with graphite furnace atomic absorption spectrometry (GFAAS) was developed for the selective sampling and measurement of Cr (VI) in water. The performance of PQAS DGT was independent of pH 3–12 and ionic strength from 1 × 10{sup −3} to 1 mol L{sup −1}. DGT validation experiments showed that Cr (VI) was measured accurately as well as selectively by PQAS DGT, whereas Cr (III) was not determined quantitatively. Compared with diphenylcarbazide spectrophotometric method (DPC), the measurement of Cr (VI) with PQAS DGT was agreement with that of DPC method in the industrial wastewater. PQAS-DGT device had been successfully deployed in local freshwater. The concentrations of Cr (VI) determined by PQAS DGT coupled with GFAAS in Nuer River, Ling River and North Lake were 0.73 ± 0.09 μg L{sup −1}, 0.50 ± 0.07 μg L{sup −1} and 0.61 ± 0.07 μg L{sup −1}, respectively. The results indicate that PQAS DGT device can be used for the selective sampling and measurement Cr (VI) in water and its detection limit is lower than that of DPC method.

  18. Suction/Inspiration against resistance or standardized Mueller maneuver : a new breathing technique to improve contrast density within the pulmonary artery: a pilot CT study.

    Science.gov (United States)

    Gutzeit, Andreas; Froehlich, Johannes M; Wälti, Stephan; Roos, Justus E; Meissnitzer, Matthias; Hergan, Klaus; von Weymarn, Constantin; Czell, David; Goyen, Matthias; Reischauer, Carolin

    2015-11-01

    Our aim was to prospectively investigate whether the recently introduced suction/inspiration against resistance breathing method leads to higher computed tomography (CT) contrast density in the pulmonary artery compared to standard breathing. The present study was approved by the Medical Ethics committee and all subjects gave written informed consent. Fifteen patients, each without suspicious lung emboli, were randomly assigned to four different groups with different breathing maneuvers (suction against resistance, Valsalva, inspiration, expiration) during routine CT. Contrast enhancement in the central and peripheral sections of the pulmonary artery were measured and compared with one another. Peripheral enhancement during suction yielded increased mean densities of 138.14 Hounsfield units (HU) (p = 0.001), compared to Valsalva and a mean density of 67.97 HU superior to inspiration (p = 0.075). Finally, suction in comparison to expiration resulted in a mean increase of 30.51 HU (p = 0.42). Central parts of pulmonary arteries presented significantly increased enhancement values (95.74 HU) for suction versus the Valsalva technique (p = 0.020), while all other mean densities were in favour of suction (versus inspiration: p = 0.201; versus expiration: p = 0.790) without reaching significance. Suction/Inspiration against resistance is a promising technique to improve contrast density within pulmonary vessels, especially in the peripheral parts, in comparison to other breathing maneuvers. • Suction/Inspiration against resistance is promising to improve contrast density within the pulmonary artery. • Patients potentially suffering pulmonary embolism are able to follow suction/inspiration against resistance. • Contrast density after suction is superior in comparison to other breathing maneuvers.

  19. High density and connectivity of a MgB2 filament made using the internal magnesium diffusion technique

    Science.gov (United States)

    Kulich, M.; Kováč, P.; Hain, M.; Rosová, A.; Dobročka, E.

    2016-03-01

    In order to allow precise and detailed physical studies of an MgB2 filament made by the internal magnesium diffusion process (IMD), a modified approach (MIMD) using a Mg tube filled with boron powder deformed into wire was introduced. The MIMD process allows easy extraction of the MgB2 filament after the final heat treatment and performance of four-probe resistive measurements and density estimation, which is not possible for standard IMD wires. The Rowell approach has been applied for the grain connectivity from R(T) data of extracted MgB2 for the first time. The filament’s density has been estimated from the precise volume measured by x-ray micro-tomography and mass. The high connectivity and density of the MgB2 filament made by the diffusion process are discussed and compared with those of filaments made by other processes.

  20. The control of plasma density profile in Tore Supra. Comparison of different fueling techniques; Controle du profil de densite dans le plasma de Tore Supra. Comparaison de differentes methodes d'alimentation en particules

    Energy Technology Data Exchange (ETDEWEB)

    Commaux, N

    2007-09-15

    The behaviour of a reactor-class plasma when fuelled using the existing techniques (gas puffing, supersonic molecular beam injection and pellet injection) is still very difficult to foresee. The present work has been initiated on Tore Supra in order to extrapolate the consequences of the different fuelling systems on ITER. Two main topics have been studied: the comparison of the plasma behaviour when fuelled using the different techniques at high Greenwald density fractions and the study of the homogenization following a pellet injection (main fuelling technique for ITER burning plasmas). The experiments at high Greenwald density fractions performed on Tore Supra showed that the plasma behaviour is very dependent on the fuelling method. The plasma energy confinement is following the scaling laws determined at low density when fuelled using pellet injection. which is better than for gas puffing and SMBI. both inducing a significant confinement loss. This behaviour is nor related to a transport modification: the ratio between effective diffusion and convection is similar to the pellet case. The difference between these shots is related only to the position of the matter source (at the edge for gas and close to the center for pellets). The study concerning the homogenization phenomena following a pellet injection aims mainly to study the {nabla}B-drift effect that expels the mater deposited by a pellet toward the low field side. A new phenomenon. which appears to be particularly important for the {nabla}B-drift during low field side injections. was then discovered: the influence of magnetic surfaces with an integer-valued safety factor (q). When the mater drifting toward low field side crosses an integer q surface. it experiences an important braking effect which stops the drift motion. It implies that the pellet material is mainly deposited on the last integer q surface crossed by the pellet during its injection. This study allows also to determine that the {nabla

  1. Studies on remote sensing method of particle size and water density distribution in mists and clouds using laser radar techniques

    Science.gov (United States)

    Shimizu, H.; Kobayasi, T.; Inaba, H.

    1979-01-01

    A method of remote measurement of the particle size and density distribution of water droplets was developed. In this method, the size of droplets is measured from the Mie scattering parameter which is defined as the total-to-backscattering ratio of the laser beam. The water density distribution is obtained by a combination of the Mie scattering parameter and the extinction coefficient of the laser beam. This method was examined experimentally for the mist generated by an ultrasonic mist generator and applied to clouds containing rain and snow. Compared with the conventional sampling method, the present method has advantages of remote measurement capability and improvement in accuracy.

  2. INFLUENCES OF SLOPE GRADIENT ON SOIL EROSION

    Institute of Scientific and Technical Information of China (English)

    刘青泉; 陈力; 李家春

    2001-01-01

    The main factors influencing soil erosion include the net rain excess, the water depth, the velocity, the shear stress of overland flows , and the erosion-resisting capacity of soil. The laws of these factors varying with the slope gradient were investigated by using the kinematic wave theory. Furthermore, the critical slope gradient of erosion was driven. The analysis shows that the critical slope gradient of soil erosion is dependent on grain size , soil bulk density , surface roughness, runoff length, net rain excess, and the friction coefficient of soil, etc. The critical slope gradient has been estimated theoretically with its range between 41. 5 °~ 50°.

  3. Image and Radiation Power Analysis Techniques for Determining Electron temperature, Liner Areal Density, and Radiated Energy in MagLIF Experiments

    Science.gov (United States)

    Evans, Matthew; Knapp, Patrick; Gomez, Matthew; Hansen, Stephanie; McBride, Ryan; MacPherson, L. Armon; Gourdain, Pierre

    2016-10-01

    We describe techniques developed to analyze filtered Time Integrated Pinhole Camera (TIPC) images to determine the axially resolved electron temperature and liner areal density at stagnation in MagLIF experiments conducted on the Z machine at Sandia National Laboratories. X-ray power detectors are analyzed to determine the absolute radiated energy. The TIPC images are co-registered using intensity based similarities. This technique is shown to provide accurate registration without the use of fiducial markings. A filtered 6-channel PCD array was used to record the radiated power at photon energies >1 keV. A model for the x-ray emission is used with the data set to perform Bayesian parameter estimation to simultaneously determine the electron temperature, liner areal density and x-ray yield with uncertainties via χ2 minimization.

  4. Bone mass density estimation: Archimede’s principle versus automatic X-ray histogram and edge detection technique in ovariectomized rats treated with germinated brown rice bioactives

    Science.gov (United States)

    Muhammad, Sani Ismaila; Maznah, Ismail; Mahmud, Rozi Binti; Esmaile, Maher Faik; Zuki, Abu Bakar Zakaria

    2013-01-01

    Background Bone mass density is an important parameter used in the estimation of the severity and depth of lesions in osteoporosis. Estimation of bone density using existing methods in experimental models has its advantages as well as drawbacks. Materials and methods In this study, the X-ray histogram edge detection technique was used to estimate the bone mass density in ovariectomized rats treated orally with germinated brown rice (GBR) bioactives, and the results were compared with estimated results obtained using Archimede’s principle. New bone cell proliferation was assessed by histology and immunohistochemical reaction using polyclonal nuclear antigen. Additionally, serum alkaline phosphatase activity, serum and bone calcium and zinc concentrations were detected using a chemistry analyzer and atomic absorption spectroscopy. Rats were divided into groups of six as follows: sham (nonovariectomized, nontreated); ovariectomized, nontreated; and ovariectomized and treated with estrogen, or Remifemin®, GBR-phenolics, acylated steryl glucosides, gamma oryzanol, and gamma amino-butyric acid extracted from GBR at different doses. Results Our results indicate a significant increase in alkaline phosphatase activity, serum and bone calcium, and zinc and ash content in the treated groups compared with the ovariectomized nontreated group (P Archimede’s principle and the edge detection technique between the treated groups (r2 = 0.737, P = 0.004). Conclusion Our study shows that GBR bioactives increase bone density, which might be via the activation of zinc formation and increased calcium content, and that X-ray edge detection technique is effective in the measurement of bone density and can be employed effectively in this respect. PMID:24187491

  5. SEMICONDUCTOR DEVICES Density-controllable nonvolatile memory devices having metal nanocrystals through chemical synthesis and assembled by spin-coating technique

    Science.gov (United States)

    Guangli, Wang; Yubin, Chen; Yi, Shi; Lin, Pu; Lijia, Pan; Rong, Zhang; Youdou, Zheng

    2010-12-01

    A novel two-step method is employed, for the first time, to fabricate nonvolatile memory devices that have metal nanocrystals. First, size-averaged Au nanocrystals are synthesized chemically; second, they are assembled into memory devices by a spin-coating technique at room temperature. This attractive approach makes it possible to tailor the diameter and control the density of nanocrystals individually. In addition, processes at room temperature prevent Au diffusion, which is a main concern for the application of metal nanocrystal-based memory. The experimental results, both the morphology characterization and the electrical measurements, reveal that there is an optimum density of nanocrystal monolayer to balance between long data retention and a large hysteresis memory window. At the same time, density-controllable devices could also feed the preferential emphasis on either memory window or retention time. All these facts confirm the advantages and novelty of our two-step method.

  6. BREAST DENSITY EVALUATION: A COMPARISON BETWEEN ASSESSMENT BY A RADIOLOGIST AND THE COMPUTER-ASSISTED THRESHOLD TECHNIQUE

    Directory of Open Access Journals (Sweden)

    George Baytchev

    2015-09-01

    Full Text Available Traditionally, mammographic density (MD of the breast has been assessed by a radiologist visually. This subjective evaluation requires significant experience to distinguish the relative proportions of the fibrous connective tissue and adipose tissue in the mammary gland correctly. The aim of this study is to compare the capabilities of the different methods (visual and computer-assisted for assessing mammographic density. Our sample in this study consists of 66 patients with digital mammography. The mammographic density has been evaluated using the four-grade scale of the American College of Radiology (ACR; visually, visually using an analog scale and semi-automated using UTHSCSA Image Tool 3.0, Image J and Adobe Photoshop CS6 software. The average mammographic density calculated using the different methods is as follows: 34.8% (from 10% to 70%; 32.1% (from 10% to 60%; 23% (from 0% to 70.9%; 22.7% (from 2.5% to 78.1% and 22.5% (from 1.5% to 72.4%. There is a strong correlation between the results obtained visually and those calculated using a computer-assisted measurement (p< 0.0001. A strong correlation was found also between the results acquired using the different semi-automated programs (p< 0.0001. Precise measurement of mammographic density is of great importance for the mammographic screening and evaluation of breast cancer risk. The semi-automated methods, used for this purpose are objective, accessible and reproducible tools and have some advantages over the subjective visual assessment.

  7. Graded/Gradient Porous Biomaterials

    Directory of Open Access Journals (Sweden)

    Xigeng Miao

    2009-12-01

    Full Text Available Biomaterials include bioceramics, biometals, biopolymers and biocomposites and they play important roles in the replacement and regeneration of human tissues. However, dense bioceramics and dense biometals pose the problem of stress shielding due to their high Young’s moduli compared to those of bones. On the other hand, porous biomaterials exhibit the potential of bone ingrowth, which will depend on porous parameters such as pore size, pore interconnectivity, and porosity. Unfortunately, a highly porous biomaterial results in poor mechanical properties. To optimise the mechanical and the biological properties, porous biomaterials with graded/gradient porosity, pores size, and/or composition have been developed. Graded/gradient porous biomaterials have many advantages over graded/gradient dense biomaterials and uniform or homogenous porous biomaterials. The internal pore surfaces of graded/gradient porous biomaterials can be modified with organic, inorganic, or biological coatings and the internal pores themselves can also be filled with biocompatible and biodegradable materials or living cells. However, graded/gradient porous biomaterials are generally more difficult to fabricate than uniform or homogenous porous biomaterials. With the development of cost-effective processing techniques, graded/gradient porous biomaterials can find wide applications in bone defect filling, implant fixation, bone replacement, drug delivery, and tissue engineering.

  8. An optimum PN code search technique for a given apriori signal location density. [PseudoNoise in radar detection

    Science.gov (United States)

    Holmes, J. K.; Woo, K. T.

    1978-01-01

    A method is presented for optimizing (minimizing) the acquisition time for a PN-type spread spectrum system when the a priori probability density function is not uniform. For an assumed a priori Gaussian density function, calculations show that the acquisition time, when the 0.5 probability acquisition time is used as a measure of acquisition time, is reduced by 40% for a cell detection probability of 0.25 and when three sweeps are used. For the same parameters and with four sweeps the acquisition time is reduced by 41%. When the acquisition time probability is set to 0.9 instead of 0.5, the reduction is 25% of the uniform sweep time.

  9. In situ speciation of dissolved inorganic antimony in surface waters and sediment porewaters: development of a thiol-based diffusive gradients in thin films technique for Sb(III).

    Science.gov (United States)

    Bennett, William W; Arsic, Maja; Welsh, David T; Teasdale, Peter R

    2016-08-10

    Antimony is a priority environmental contaminant typically present as either the trivalent (Sb(III)) or the pentavalent (Sb(V)) oxidation state in aquatic systems. Both the toxicity and mobility of antimony are affected by its speciation, and thus the accurate measurement of antimony speciation is essential for investigating the behaviour of this contaminant in aquatic systems. Here we present a diffusive gradients in thin films (DGT) technique, which utilises a binding layer containing a thiol-based adsorbent (3-mercaptopropyl functionalised silica gel), for the selective measurement of Sb(III) in surface waters and sediment porewaters. We also evaluated the Metsorb DGT technique, which has been previously reported to accurately measure Sb(V), for its ability to accumulate Sb(III) and thus allow the measurement of total inorganic antimony. Both the mercapto-silica and Metsorb DGT techniques showed a high affinity for Sb(III), with uptake efficiencies >97%. Elution efficiencies of 86.9 ± 2.6% and 88.1 ± 1.2% were obtained for mercapto-silica and Metsorb, respectively, with 1 mol L(-1) H2O2 in 1 mol L(-1) NaOH. The accumulation of Sb(III) by these DGT techniques was linear with time (R(2) > 0.99) and unaffected by pH (4.07-8.05), ionic strength (0.001-1.0 mol L(-1) NaCl), bicarbonate (1-15 mmol L(-1)), and an artificial seawater matrix (pH 8.34; salinity 34.8). Finally, the mercapto-silica DGT technique was applied to measure porewater concentrations of Sb(III) and As(III) in a contaminated freshwater sediment at high resolution.

  10. Techniques, tools and best practices for ligand electron-density analysis and results from their application to deposited crystal structures.

    Science.gov (United States)

    Pozharski, Edwin; Weichenberger, Christian X; Rupp, Bernhard

    2013-02-01

    As a result of substantial instrumental automation and the continuing improvement of software, crystallographic studies of biomolecules are conducted by non-experts in increasing numbers. While improved validation almost ensures that major mistakes in the protein part of structure models are exceedingly rare, in ligand-protein complex structures, which in general are most interesting to the scientist, ambiguous ligand electron density is often difficult to interpret and the modelled ligands are generally more difficult to properly validate. Here, (i) the primary technical reasons and potential human factors leading to problems in ligand structure models are presented; (ii) the most common categories of building errors or overinterpretation are classified; (iii) a few instructive and specific examples are discussed in detail, including an electron-density-based analysis of ligand structures that do not contain any ligands; (iv) means of avoiding such mistakes are suggested and the implications for database validity are discussed and (v) a user-friendly software tool that allows non-expert users to conveniently inspect ligand density is provided.

  11. Biomimetic Gradient Polymers with Enhanced Damping Capacities.

    Science.gov (United States)

    Wang, Dong; Zhang, Huan; Guo, Jing; Cheng, Beichen; Cao, Yuan; Lu, Shengjun; Zhao, Ning; Xu, Jian

    2016-04-01

    Designing gradient structures, mimicking biological materials, such as pummelo peels and tendon, is a promising strategy for developing advanced materials with superior energy damping capacities. Here a facile and effective approach for fabricating polymers with composition gradients at millimeter length scale is presented. The gradient thiol-ene polymers (TEPs) are created by the use of density difference of ternary thiol-ene-ene precursors and the subsequent photo-crosslinking via thiol-ene reaction. The compositional gradients are analyzed via differential scanning calorimeter (DSC), compressive modulus testing, atomic force microscopy (AFM) indentation, and swelling measurements. In contrast to homogeneous TEPs networks, the resultant gradient polymer shows a broader effective damping temperature range combining with good mechanical properties. The present result provides an effective route toward high damping materials by the fabrication of gradient structures.

  12. Optical phase distortion due to turbulent-fluid density fields - Quantification using the small-aperture beam technique

    Science.gov (United States)

    Jumper, E. J.; Hugo, R. J.

    1992-07-01

    This paper discusses the small-aperture beam technique, a relatively new way of experimentally quantifying optically-active, turbulent-fluid-flow-induced optical degradation. The paper lays out the theoretical basis for the technique, and the relationship of the measured jitter of the beam to optical path difference. A numerical simulation of a two-dimensional heated jet is used to explore the validity of beam jitter to obtain optical path difference in a flow region where eddy production constitutes the major character of the 'turbulent' flow field.

  13. An Inexpensive Digital Gradient Controller for HPLC.

    Science.gov (United States)

    Brady, James E.; Carr, Peter W.

    1983-01-01

    Use of gradient elution techniques in high performance liquid chromatography (HPLC) is often essential for direct separation of complex mixtures. Since most commercial controllers have features that are of marginal value for instructional purposes, a low-cost controller capable of illustrating essential features of gradient elution was developed.…

  14. An Inexpensive Digital Gradient Controller for HPLC.

    Science.gov (United States)

    Brady, James E.; Carr, Peter W.

    1983-01-01

    Use of gradient elution techniques in high performance liquid chromatography (HPLC) is often essential for direct separation of complex mixtures. Since most commercial controllers have features that are of marginal value for instructional purposes, a low-cost controller capable of illustrating essential features of gradient elution was developed.…

  15. Differentiation between simple cyst and hepatic hemangioma utilizing T2-weighted magnetic resonance imaging with gradient-echo (b-FFE) technique

    Energy Technology Data Exchange (ETDEWEB)

    Burim, Carolina Valente; D' Ippolito, Giuseppe [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. de Diagnostico por Imagem]. E-mail: scoposl@uol.com.br; Pecci Neto, Luiz; Torlai, Fabiola Goda; Tiferes, Dario Ariel [Laboratorio Fleury, Sao Paulo, SP (Brazil). Centro de Medicina Diagnostica

    2008-11-15

    Objective: to establish the role of MRI T2-weighted sequences in the differentiation between simple cysts and hepatic hemangiomas. Materials and methods: a double-blinded, prospective, observational, cross sectional study evaluated 52 patients with 91 hepatic lesions (34 simple cysts and 57 hemangiomas) submitted to abdominal magnetic resonance imaging. The combined analysis of all sequences was considered as the golden-standard. TSE sequences with long echo trains and b-FFE sequences were subjectively analyzed by two independent observers for differentiating cysts from hemangiomas. The kappa test ({kappa}) was utilized in the analysis of the methods accuracy and inter- and intra-observer agreement (p < 0.05{sup *}). Results: cysts and hemangiomas dimensions ranged respectively between 0.5 and 6.5 cm (mean 1.89 cm), and 0.8 and 11 cm (mean = 2.62 cm). The analysis of the sequences with long-TE and the golden-standard demonstrated a non-statistically significant agreement (k: 0.00-0.10). The agreement between the evaluation of the b-FFE sequence and the golden-standard ranged from substantial ({kappa}: 0.62-0.71) to almost perfect ({kappa}: 0.86) for both observers. The inter- and intra-observer agreement for the b-FFE sequence ranged from substantial ({kappa}: 0.62-0.70) to almost perfect ({kappa}: 0.85-0.91). Conclusion: T2-weighted images acquired with the b-FFE technique present a high accuracy and reproducibility in the differentiation between cysts and hepatic hemangiomas. (author)

  16. Effects of forest road amelioration techniques on soil bulk density, surface runoff, sediment transport, soil moisture and seedling growth

    Science.gov (United States)

    Randy K. Kolka; Mathew F. Smidt

    2004-01-01

    Although numerous methods have been used to retire roads, new technologies have evolved that can potentially ameliorate soil damage, lessen ,the generation of nonpoint source pollution and increase tree productivity on forest roads. In this study we investigated the effects of three forest road amelioration techniques, subsoiling, recontouring and traditional...

  17. A systematic determination of diffusion coefficients of trace elements in open and restricted diffusive layers used by the diffusive gradients in a thin film technique.

    Science.gov (United States)

    Shiva, Amir Houshang; Teasdale, Peter R; Bennett, William W; Welsh, David T

    2015-08-12

    A systematic comparison of the diffusion coefficients of cations (Al, Cd, Co, Cu, Mn, Ni, Pb, Zn) and oxyanions (Al, As, Mo, Sb, V, W) in open (ODL) and restricted (RDL) diffusive layers used by the DGT technique was undertaken. Diffusion coefficients were measured using both the diffusion cell (Dcell) method at pH 4.00 and the DGT time-series (D(DGT)) method at pH 4.01 and 7.04 (pH 8.30 was used instead of 7.04 for Al) using the Chelex-Metsorb mixed binding layer. The performance of Chelex-Metsorb as a new DGT binding layer for Al uptake was also evaluated for the first time. Reasonable agreement was observed between D(cell) and D(DGT) measurements for both ODL and RDL, except for V and W. The ratios of D(cell)/D(DGT) for V of 0.44 and 0.39, and for W of 0.66 and 0.63 with ODL and RDL respectively, were much lower due to the formation of a high proportion of polyoxometalate species at the higher concentrations required with the D(cell) measurements. This is the first time that D values have been reported for several oxyanions using RDL. Except for Al at pH 8.30 with ODL, all D(DGT) measurements were retarded relative to diffusion coefficients in water (DW) for both diffusive hydrogels. Diffusion in RDL was further retarded compared with ODL, for all elements (0.66-0.78) with both methods. However, the degree of retardation observed changed for cations and anions at each pH. At pH 7.04 cations had a slightly higher D(DGT) and oxyanions had a slightly lower D(DGT) than at pH 4.01 for both ODL and RDL. It is proposed that this is due to partial formation of acrylic acid functional groups (pKa ≈4.5), which would be fully deprotonated at pH 7.04 (negative) and mostly protonated at pH 4.01 (neutral). As Al changes from being cationic at pH 4.01 to anionic at pH 8.30 the results were more complex.

  18. A systematic determination of diffusion coefficients of trace elements in open and restricted diffusive layers used by the diffusive gradients in a thin film technique

    Energy Technology Data Exchange (ETDEWEB)

    Shiva, Amir Houshang; Teasdale, Peter R., E-mail: p.teasdale@griffith.edu.au; Bennett, William W.; Welsh, David T.

    2015-08-12

    A systematic comparison of the diffusion coefficients of cations (Al, Cd, Co, Cu, Mn, Ni, Pb, Zn) and oxyanions (Al, As, Mo, Sb, V, W) in open (ODL) and restricted (RDL) diffusive layers used by the DGT technique was undertaken. Diffusion coefficients were measured using both the diffusion cell (D{sub cell}) method at pH 4.00 and the DGT time-series (D{sub DGT}) method at pH 4.01 and 7.04 (pH 8.30 was used instead of 7.04 for Al) using the Chelex-Metsorb mixed binding layer. The performance of Chelex-Metsorb as a new DGT binding layer for Al uptake was also evaluated for the first time. Reasonable agreement was observed between D{sub cell} and D{sub DGT} measurements for both ODL and RDL, except for V and W. The ratios of D{sub cell}/D{sub DGT} for V of 0.44 and 0.39, and for W of 0.66 and 0.63 with ODL and RDL respectively, were much lower due to the formation of a high proportion of polyoxometalate species at the higher concentrations required with the D{sub cell} measurements. This is the first time that D values have been reported for several oxyanions using RDL. Except for Al at pH 8.30 with ODL, all D{sub DGT} measurements were retarded relative to diffusion coefficients in water (D{sub W}) for both diffusive hydrogels. Diffusion in RDL was further retarded compared with ODL, for all elements (0.66–0.78) with both methods. However, the degree of retardation observed changed for cations and anions at each pH. At pH 7.04 cations had a slightly higher D{sub DGT} and oxyanions had a slightly lower D{sub DGT} than at pH 4.01 for both ODL and RDL. It is proposed that this is due to partial formation of acrylic acid functional groups (pK{sub a} ≈4.5), which would be fully deprotonated at pH 7.04 (negative) and mostly protonated at pH 4.01 (neutral). As Al changes from being cationic at pH 4.01 to anionic at pH 8.30 the results were more complex. - Highlights: • Determining elemental diffusion coefficients in open and restricted diffusive gels. • The DGT

  19. Gradient Descent Bit Flipping Algorithms for Decoding LDPC Codes

    OpenAIRE

    Wadayama, Tadashi; Nakamura, Keisuke; Yagita, Masayuki; Funahashi, Yuuki; Usami, Shogo; Takumi, Ichi

    2007-01-01

    A novel class of bit-flipping (BF) algorithms for decoding low-density parity-check (LDPC) codes is presented. The proposed algorithms, which are called gradient descent bit flipping (GDBF) algorithms, can be regarded as simplified gradient descent algorithms. Based on gradient descent formulation, the proposed algorithms are naturally derived from a simple non-linear objective function.

  20. A tropical cyclone dynamic initialization technique using high temporal and spatial density atmospheric motion vectors and airborne field campaign data

    Science.gov (United States)

    Hendricks, E. A.; Bell, M. M.; Elsberry, R. L.; Velden, C.

    2016-12-01

    A new tropical cyclone dynamic initialization technique is described and tested. The technique uses the triple-nested Coupled Ocean-Atmosphere Mesoscale Prediction System-Tropical Cyclones (COAMPS-TC) (with horizontal grid spacings of 45-,15-, and 5-km, respectively) in conjunction with the Spline Analysis at Mesoscale Utilizing Radar and Aircraft Instrumentation (SAMURAI). A proof-of-concept demonstration of this technique is given for Hurricane Joaquin from the Office of Naval Research (ONR) Tropical Cyclone Intensity (TCI) field program conducted in 2015. High spatial and temporal resolution atmospheric motion vectors (AMVs), dropwindsondes from the Yankee Environmental Systems High Definition Sounding System (HDSS), and surface wind speed retrievals from the Hurricane Imaging Radiometer (HIRAD) are ingested into SAMURAI to produce increments, which are then used by the COAMPS-TC dynamic initialization scheme to produce consistent dynamic and thermodynamically balanced fields. This high temporal resolution (order of 10-15 minutes) incremental dynamic initialization procedure has advantages over conventional methods in that a bogus vortex is not used, and existing asymmetries (including convective heating and upper and low level wind asymmetries) that exist in the TC are retained. The use of dynamic initialization also ensures improved vortex and environment balance, and consistency with the model physics. A preliminary verification of this new TC initialization scheme will be presented for the initialization and forecast of Hurricane Joaquin (2015).

  1. Perturbative running of the twisted Yang-Mills coupling in the gradient flow scheme

    CERN Document Server

    Bribian, Eduardo I

    2016-01-01

    We report on our ongoing computation of the perturbative running of the Yang-Mills coupling using gradient flow techniques. In particular, we use the gradient flow method with twisted boundary conditions to perform a perturbative expansion of the expectation value of the Yang-Mills energy density up to fourth order in the coupling at finite flow time. We regularise the resulting integrals using dimensional regularisation, and reproduce the universal coefficient of the 1/{\\epsilon} term in the relation between bare and renormalised couplings. The computation of the finite part leading to a determination of the {\\Lambda} parameter in this scheme is underway.

  2. Modelling risk of tick exposure in southern Scandinavia using machine learning techniques, satellite imagery, and human population density maps

    DEFF Research Database (Denmark)

    Kjær, Lene Jung; Korslund, L.; Kjelland, V.

    Vector-borne diseases such as Lyme disease and tick-borne encephalitis have become more common in recent decades and present a real health problem in many parts of Europe. Risk assessment, control, and prevention of these diseases require a better understanding of vector abundance as well as risk...... factors determining human exposure to ticks. There is a great need for analyses and models that can predict how vectors and their associated diseases are distributed and how this relates to high risk areas for human exposure.As a part of the ScandTick Innovation project, we surveyed ticks at approximately...... distribution (probability of presence) in southern Scandinavia. Together with the predicted distribution maps, we used human density maps to determine areas with high risk of exposure to ticks. For nymphs, the predicted distribution found corresponded well with known distributions of ticks in Scandinavia...

  3. Bone mass density estimation: Archimede’s principle versus automatic X-ray histogram and edge detection technique in ovariectomized rats treated with germinated brown rice bioactives

    Directory of Open Access Journals (Sweden)

    Muhammad SI

    2013-10-01

    Full Text Available Sani Ismaila Muhammad,1,2 Ismail Maznah,1,3 Rozi Binti Mahmud,4 Maher Faik Esmaile,5 Zuki Abu Bakar Zakaria6 1Laboratory of Molecular Biomedicine, Institute of Bioscience, 2Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria; 3Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, 4Department of Radiology, Faculty of Medicine and Health Sciences, 5Department of Electrical and Electronic Engineering, Faculty of Engineering, 6Department of Pre-clinical Studies, Faculty of Veterinary Medicine, University Putra Malaysia, Selangor, Malaysia Background: Bone mass density is an important parameter used in the estimation of the severity and depth of lesions in osteoporosis. Estimation of bone density using existing methods in experimental models has its advantages as well as drawbacks. Materials and methods: In this study, the X-ray histogram edge detection technique was used to estimate the bone mass density in ovariectomized rats treated orally with germinated brown rice (GBR bioactives, and the results were compared with estimated results obtained using Archimede’s principle. New bone cell proliferation was assessed by histology and immunohistochemical reaction using polyclonal nuclear antigen. Additionally, serum alkaline phosphatase activity, serum and bone calcium and zinc concentrations were detected using a chemistry analyzer and atomic absorption spectroscopy. Rats were divided into groups of six as follows: sham (nonovariectomized, nontreated; ovariectomized, nontreated; and ovariectomized and treated with estrogen, or Remifemin®, GBR-phenolics, acylated steryl glucosides, gamma oryzanol, and gamma amino-butyric acid extracted from GBR at different doses. Results: Our results indicate a significant increase in alkaline phosphatase activity, serum and bone calcium, and zinc and ash content in the treated groups compared with the ovariectomized

  4. Conversion of a transverse density modulation into a longitudinal phase space modulation using an emittance exchange technique

    CERN Document Server

    Sun, Y -E; Johnson, A; Lumpkin, A; Ruan, J; Thurman-Keup, R

    2010-01-01

    We report on an experiment to produce a train of sub-picosecond microbunches using a transverse-to-longitudinal emittance exchange technique. The generation of a modulation on the longitudinal phase space is done by converting an initial horizontal modulation produced using a multislits mask. The preliminary experimental data clearly demonstrate the conversion process. To date only the final energy modulation has been measured. However numerical simulations, in qualitative agreement with the measurements, indicate that the conversion process should also introduce a temporal modulation.

  5. Lung density

    DEFF Research Database (Denmark)

    Garnett, E S; Webber, C E; Coates, G

    1977-01-01

    The density of a defined volume of the human lung can be measured in vivo by a new noninvasive technique. A beam of gamma-rays is directed at the lung and, by measuring the scattered gamma-rays, lung density is calculated. The density in the lower lobe of the right lung in normal man during quiet...... breathing in the sitting position ranged from 0.25 to 0.37 g.cm-3. Subnormal values were found in patients with emphsema. In patients with pulmonary congestion and edema, lung density values ranged from 0.33 to 0.93 g.cm-3. The lung density measurement correlated well with the findings in chest radiographs...... but the lung density values were more sensitive indices. This was particularly evident in serial observations of individual patients....

  6. SEPARATION OF X-BEARING BOVINE SPERM BY CENTRIFUGATION IN CONTINUOUS PERCOLL AND OPTIPREP DENSITY GRADIENT: EFFECT IN SPERM VIABILITY AND IN VITRO EMBRYO PRODUCTION SEPARAÇÃO DE ESPERMATOZOIDES PORTADORES DO CROMOSSOMO X BOVINO POR CENTRIFUGAÇÃO EM GRADIENTE DE DENSIDADE CONTÍNUO DE PERCOLL E OPTIPREP: EFEITO SOBRE A VIABILIDADE ESPERMÁTICA E NA PRODUÇÃO IN VITRO DE EMBRIÕES

    Directory of Open Access Journals (Sweden)

    Aline Costa Lucio

    2009-07-01

    Full Text Available

    The aim of this study was to separate X-bearing bovine sperm by continuous Percoll and OptiPrep density gradients and to validate the sexing of resultant in vitro produced embryos by Polimerase Chain Reaction (PCR. Frozen/thawed sperm was layered on density gradients which were previously prepared in polystyrene tubes, 24 h before procedures and maintained at 4 °C. The tubes were centrifuged at 500 x g for 15 min at 22 °C. Supernatants were gently aspirated and the sperm recovered from the bottom of the tubes. Viability and integrity of sperm were evaluated by Trypan Blue/Giemsa stain. Cleavage and blastocyst rates were determined by in vitro production of embryos and PCR was performed for identification of the embryos’ genetic sex. No damage in viability and acrossomal integrity and in cleavage and blastocyst rates was found in the Percoll and OptiPrep treatment compared to the non-centrifuged group (P>0.05. The percentage of female embryos in the Percoll and OptiPrep group was 63.0 and 47.6%, respectively. The female embryos in control group were 48.7%. A sexual deviation in the Percoll density gradient was achieved without reduction of sperm viability and in vitro production rates.

    KEY WORDS: Bovine, centrifugation, in vitro production of embryos, PCR, X-bearing sperm.

    O objetivo deste estudo foi separar espermatozoides bovinos portadores do cromossomo X pela centrifugação em gradiente de densidade contínuo de Percoll e OptiPrep, e validar a sexagem pela reação em cadeia da polimerase (PCR, dos embriões produzidos in vitro. Para a sexagem, espermatozoides descongelados foram depositados nos gradientes de densidade, previamente preparados, em tubos de poliestireno, 24 horas antes da sexagem e mantidos a 4°C. Centrifugou-se a 500 x g por quinze minutos a 22°C. Os sobrenadantes foram aspirados, e os espermatozoides recuperados do

  7. Evaluation of bone-to-implant contact and bone density adjacent to titanium implants using a stereological technique on ground sections

    DEFF Research Database (Denmark)

    Balatsouka, Dimitra; Gotfredsen, Klaus; Gundersen, Hans Jørgen Gottlieb

    2006-01-01

    using stereological principles. The aim of the study was to describe an unbiased design for evaluating boneto- implant contact (BIC) and peri-implant bone density (BD-i) in three-dimensions. The unbiased design was based on a fixed axis vertical random sampling technique. Three bone-implant blocks were...... collected from 3 rabbits. Four sections were obtained from each animal using a fixed axis vertical random sampling technique. The BIC was estimated by creating a stereological method based on a systematic test line set. The BD-i was estimated using a design based on a systematic point set. The efficiency......When bone implants have to be examined in situ ground sections are required. Histomorphometric measurements are usually performed on two-dimensional sections, causing biased results when they are wrongly extrapolated to 3D without any knowledge of stereology. Unbiased results can only be obtained...

  8. Evaluation of bone-to-implant contact and bone density adjacent to titanium implants using a stereological technique on ground sections

    DEFF Research Database (Denmark)

    Balatsouka, Dimitra; Gotfredsen, Klaus; Gundersen, Hans Jørgen Gottlieb

    2006-01-01

    When bone implants have to be examined in situ ground sections are required. Histomorphometric measurements are usually performed on two-dimensional sections, causing biased results when they are wrongly extrapolated to 3D without any knowledge of stereology. Unbiased results can only be obtained...... using stereological principles. The aim of the study was to describe an unbiased design for evaluating boneto- implant contact (BIC) and peri-implant bone density (BD-i) in three-dimensions. The unbiased design was based on a fixed axis vertical random sampling technique. Three bone-implant blocks were...... collected from 3 rabbits. Four sections were obtained from each animal using a fixed axis vertical random sampling technique. The BIC was estimated by creating a stereological method based on a systematic test line set. The BD-i was estimated using a design based on a systematic point set. The efficiency...

  9. 密度梯度离心结合贴壁法培养成年大鼠骨髓间充质干细胞的生物学特性%Biological characteristics of bone marrow-derived mesenchymal stem cells cultured by density gradient centrifugation combined with adherence in adult rats

    Institute of Scientific and Technical Information of China (English)

    杨玉霞; 郑健樑; 张平; 林健贤; 张文忻

    2007-01-01

    BACKGROUND: It is important to study the methods of culturing bone marrow-derived mesenchymal stem cells (MSCs) to obtain a great amount of high purity MSCs for applying ocular tissues constructed by tissue engineering technique to treat eye diseases.OBJECTTVE: To separate and culture in vitro MSCs from bone marrow of the adult rats by density gradient centrifugation combined with adherence culture, and observe the growing characteristics and the possibility of mass multiplication.DESIGN: A completely randomized grouping design/repetitive measuring experiment.SETTING: Pathological laboratory, Zhongshan Ophthalmic Center, Sun Yat-sen University.MATERIALS: Four six-week-old SD rats about 250 g, grade Ⅱ of cleaning, were provided by the Animal Center of Sun Yat-sen University [certificate number: SCSK(Yue)2004/0011], about 250 g each rat and there was no limit to the sex. The main reagents and instruments included low sugar Dulbecco modified Eagle culture medium (DMEM/F12, American Gibco Corporation), trypsin (fetal bovine serum (FBS, Hangzhou Sijiqing Bio-Engineering Material Research Institute), American Gibco Corporation), disodium edetate, lymphocyte separating medium, fibronectin, CD44, CD34, CD31 monoclonal antibodies, two-step-method kit for immunohistochemistry (Beijing Zhong shan Biotechnology Corporation).METHODS: This experiment was conducted at the Key laboratory of Ophthalmology (Sun Yat-sen University), Ministry of ethanol (750 g/L) for 10 minutes. Under aseptic condition, the medullaris cavitas was exposed, the syringe containing application m edium was directly punctured into the femoral cavity, the cells in the medullaris cavitas were washed out with the culture medium containing heparin and taken as the cell suspension. The bone marrow-derived MSCs were separated and purified by density gradient centrifugation combined with adherence culture, and the growing conditions of the wells. When the cells had generally connected with each other, they were

  10. "Cuts in Action": A High-Density EEG Study Investigating the Neural Correlates of Different Editing Techniques in Film.

    Science.gov (United States)

    Heimann, Katrin S; Uithol, Sebo; Calbi, Marta; Umiltà, Maria A; Guerra, Michele; Gallese, Vittorio

    2016-11-24

    In spite of their striking differences with real-life perception, films are perceived and understood without effort. Cognitive film theory attributes this to the system of continuity editing, a system of editing guidelines outlining the effect of different cuts and edits on spectators. A major principle in this framework is the 180° rule, a rule recommendation that, to avoid spectators' attention to the editing, two edited shots of the same event or action should not be filmed from angles differing in a way that expectations of spatial continuity are strongly violated. In the present study, we used high-density EEG to explore the neural underpinnings of this rule. In particular, our analysis shows that cuts and edits in general elicit early ERP component indicating the registration of syntactic violations as known from language, music, and action processing. However, continuity edits and cuts-across the line differ from each other regarding later components likely to be indicating the differences in spatial remapping as well as in the degree of conscious awareness of one's own perception. Interestingly, a time-frequency analysis of the occipital alpha rhythm did not support the hypothesis that such differences in processing routes are mainly linked to visual attention. On the contrary, our study found specific modulations of the central mu rhythm ERD as an indicator of sensorimotor activity, suggesting that sensorimotor networks might play an important role. We think that these findings shed new light on current discussions about the role of attention and embodied perception in film perception and should be considered when explaining spectators' different experience of different kinds of cuts.

  11. Gradient chromatofocusing. versatile pH gradient separation of proteins in ion-exchange HPLC: characterization studies.

    Science.gov (United States)

    Shan, Lian; Anderson, David J

    2002-11-01

    A new chromatofocusing technique called gradient chromatofocusing is characterized. Gradient chromatofocusing generates linear pH gradients on anion-exchange columns with inexpensive low molecular mass buffer components via HPLC gradient mixing. Gradient chromatofocusing results are compared with that of conventional chromatofocusing in the chromatography of several proteins on a Mono P column, including beta-lactoglobulin A and B, ovalbumin, BSA, and conalbumin. Gradient chromatofocusing shows superior performance, with resolution increases greater than 3-fold being realized for the entire protein mixture and up to 25-fold for a particular protein pair. This performance superiority arises from inherent advantages in the gradient chromatofocusing technique in optimizing conditions pertinent to separation, including buffer concentration and pH gradient slope. These resolution gains arise from both increases in separation factor and decreases in peak width achieved with the pH gradient chromatofocusing technique through the manipulation of buffer concentration and the pH gradient profile. Gradient chromatofocusing is also compared with conventional NaCl gradient ion-exchange chromatography using the same Mono P column, demonstrating 3-fold resolution gains, resulting from a 3-fold decrease in peak width. The present work demonstrates the significantly improved performance that gradient chromatofocusing has in protein separations compared to other ion-exchange chromatographic techniques. Mechanisms for the various effects are discussed.

  12. The gradient flow in a twisted box

    Energy Technology Data Exchange (ETDEWEB)

    Ramos, Alberto [Deutsches Elektronen-Synchrotron (DESY), Zeuthen (Germany). John von Neumann-Inst. fuer Computing NIC

    2013-08-15

    We study the perturbative behavior of the gradient flow in a twisted box. We apply this information to define a running coupling using the energy density of the flow field. We study the step-scaling function and the size of cutoff effects in SU(2) pure gauge theory. We conclude that the twisted gradient flow running coupling scheme is a valid strategy for step-scaling purposes due to the relatively mild cutoff effects and high precision.

  13. CdS/Cd Te solar cells. Part I. Solar cells processed by the gradient recrystallization and growth technique (GREG); Celdas solares de heterounion de CdS/CdTe. Parte I. Celdas solares procesadas por la tecnica GREG

    Energy Technology Data Exchange (ETDEWEB)

    Tufino V, M.; Contreras P, G.; Albor A, M.L.; Gonzalez T, M.A. [Escuela Superior de Fisica y Matematicas, Instituto Politecnico Nacional , 07738 Mexico D.F. (Mexico); Compaan, A.D. [Department of Physics and Astronomy and Center for Materials Science and Engineering, The University of Toledo, Toledo OH 43606 (United States)

    1998-12-31

    In this paper we present the processing and characterization of thin film CdS/Cd Te solar cells obtained by the gradient recrystallization and growth technique, GREG. The cells were deposited on soda-lime LOF{sup TM} conducting glass substrates and Cu-Au contacts were evaporated on top of the Cd Te film. The films deposition conditions were: for CdS the source temperature T{sub f} varied between 750 and 800 Centigrade and the substrate temperature T{sub s} varied between 480 and 550 Centigrade, while for Cd Te T{sub f} varied between 570 and 650 Centigrade and T{sub s} from 460 to 480 Centigrade; both films were deposited under a constant Ar gas pressure. The films were characterized by X-ray diffraction, atomic force microscopy, optical absorption and photoluminescence . Both CdS and Cd Te films were polycrystalline with preferential orientation in the (002) direction for CdS and in the (111) direction for Cd Te; the grain size ranges for the films were 0.2-1 {mu} m for CdS and 0.5-5 {mu} m for Cd Te. The solar cell photoconductive parameters were determined yielding the best cell performance values of V{sub OC} = 0.7 V, J{sub SC} = 31 m A/cm{sup 2} , f f = 50% , SQE{sub max} = 0.6 elect./photon at 550 nm and 8 % solar energy conversion efficiency. (Author)

  14. Tubing-Electrospinning: A One-Step Process for Fabricating Fibrous Matrices with Spatial, Chemical, and Mechanical Gradients.

    Science.gov (United States)

    Kim, Jung-Suk; Im, Byung Gee; Jin, Gyuhyung; Jang, Jae-Hyung

    2016-08-31

    Guiding newly generated tissues in a gradient pattern, thereby precisely mimicking inherent tissue morphology and subsequently arranging the intimate networks between adjacent tissues, is essential to raise the technical levels of tissue engineering and facilitate its transition into the clinic. In this study, a straightforward electrospinning method (the tubing-electrospinning technique) was developed to create fibrous matrices readily with diverse gradient patterns and to induce patterned cellular responses. Gradient fibrous matrices can be produced simply by installing a series of polymer-containing lengths of tubing into an electrospinning circuit and sequentially processing polymers without a time lag. The loading of polymer samples with different characteristics, including concentration, wettability, and mechanical properties, into the tubing system enabled unique features in fibrous matrices, such as longitudinal gradients in fiber density, surface properties, and mechanical stiffness. The resulting fibrous gradients were shown to arrange cellular migration and residence in a gradient manner, thereby offering efficient cues to mediate patterned tissue formation. The one-step process using tubing-electrospinning apparatus can be used without significant modifications regardless of the type of fibrous gradient. Hence, the tubing-electrospinning system can serve as a platform that can be readily used by a wide-range of users to induce patterned tissue formation in a gradient manner, which will ultimately improve the functionality of tissue engineering scaffolds.

  15. Electric field gradients in Hg compounds

    DEFF Research Database (Denmark)

    Arcisauskaité, Vaida; Knecht, Stefan; Sauer, Stephan P. A.

    2012-01-01

    We examine the performance of Density Functional Theory (DFT) approaches based on the Zeroth-Order Regular Approximation (ZORA) Hamiltonian (with and without inclusion of spinorbit coupling) for predictions of electric ¿eld gradients (EFGs) at the heavy atom Hg nucleus. This is achieved by compar......We examine the performance of Density Functional Theory (DFT) approaches based on the Zeroth-Order Regular Approximation (ZORA) Hamiltonian (with and without inclusion of spinorbit coupling) for predictions of electric ¿eld gradients (EFGs) at the heavy atom Hg nucleus. This is achieved...

  16. Dual fuel gradients in uranium silicide plates

    Energy Technology Data Exchange (ETDEWEB)

    Pace, B.W. [Babock and Wilcox, Lynchburg, VA (United States)

    1997-08-01

    Babcock & Wilcox has been able to achieve dual gradient plates with good repeatability in small lots of U{sub 3}Si{sub 2} plates. Improvements in homogeneity and other processing parameters and techniques have allowed the development of contoured fuel within the cladding. The most difficult obstacles to overcome have been the ability to evaluate the bidirectional fuel loadings in comparison to the perfect loading model and the different methods of instilling the gradients in the early compact stage. The overriding conclusion is that to control the contour of the fuel, a known relationship between the compact, the frames and final core gradient must exist. Therefore, further development in the creation and control of dual gradients in fuel plates will involve arriving at a plausible gradient requirement and building the correct model between the compact configuration and the final contoured loading requirements.

  17. A new family of conjugate gradient methods

    Science.gov (United States)

    Shi, Zhen-Jun; Guo, Jinhua

    2009-02-01

    In this paper we develop a new class of conjugate gradient methods for unconstrained optimization problems. A new nonmonotone line search technique is proposed to guarantee the global convergence of these conjugate gradient methods under some mild conditions. In particular, Polak-Ribiére-Polyak and Liu-Storey conjugate gradient methods are special cases of the new class of conjugate gradient methods. By estimating the local Lipschitz constant of the derivative of objective functions, we can find an adequate step size and substantially decrease the function evaluations at each iteration. Numerical results show that these new conjugate gradient methods are effective in minimizing large-scale non-convex non-quadratic functions.

  18. In situ selective determination of methylmercury in river water by diffusive gradient in thin films technique (DGT) using baker's yeast (Saccharomyces cerevisiae) immobilized in agarose gel as binding phase

    Energy Technology Data Exchange (ETDEWEB)

    Tafurt-Cardona, Makenly [Programa de Pós-graduação em Geociências e Meio Ambiente, Instituto de Geociências e Ciências Exatas, UNESP – Univ. Estadual Paulista, Av. 24-A, 1515, CEP: 13506-900, Rio Claro, SP (Brazil); Centro de Estudos Ambientais, UNESP – Univ. Estadual Paulista, Av. 24-A, 1515, CEP: 13506-900, Rio Claro, SP (Brazil); Eismann, Carlos Eduardo; Suárez, Carlos Alfredo [Centro de Estudos Ambientais, UNESP – Univ. Estadual Paulista, Av. 24-A, 1515, CEP: 13506-900, Rio Claro, SP (Brazil); Menegário, Amauri Antonio, E-mail: amenega@rc.unesp.br [Programa de Pós-graduação em Geociências e Meio Ambiente, Instituto de Geociências e Ciências Exatas, UNESP – Univ. Estadual Paulista, Av. 24-A, 1515, CEP: 13506-900, Rio Claro, SP (Brazil); Centro de Estudos Ambientais, UNESP – Univ. Estadual Paulista, Av. 24-A, 1515, CEP: 13506-900, Rio Claro, SP (Brazil); Silva Luko, Karen [Programa de Pós-graduação em Geociências e Meio Ambiente, Instituto de Geociências e Ciências Exatas, UNESP – Univ. Estadual Paulista, Av. 24-A, 1515, CEP: 13506-900, Rio Claro, SP (Brazil); Centro de Estudos Ambientais, UNESP – Univ. Estadual Paulista, Av. 24-A, 1515, CEP: 13506-900, Rio Claro, SP (Brazil); and others

    2015-08-05

    Saccharomyces cerevisiae immobilized in agarose gel as binding phase and polyacrylamide as diffusive layer in the diffusive gradient in thin films technique (DGT) was used for selective determination of methylmercury (MeHg). Deployment tests showed good linearity in mass uptake up to 48 h (3276 ng). When coupling the DGT technique with Cold Vapor Atomic Fluorescence Spectrometry, the method has a limit of detection of 0.44 ng L{sup −1} (pre concentration factor of 11 for 48 h deployment). Diffusion coefficient of 7.03 ± 0.77 × 10{sup −6} cm{sup 2} s{sup −1} at 23 °C in polyacrylamide gel (pH = 5.5 and ionic strength = 0.05 mol L{sup −1} NaCl) was obtained. Influence of ionic strength (from 0.0005 mol L{sup −1} to 0.1 mol L{sup −1} NaCl) and pH (from 3.5 to 8.5) on MeHg uptake were evaluated. For these range, recoveries of 84–105% and 84–98% were obtained for ionic strength and pH respectively. Potential interference due to presence of Cu, Fe, Mn, Zn was also assessed showing good recoveries (70–87%). The selectivity of the proposed approach was tested by deployments in solutions containing MeHg and Hg(II). Results obtained showed recoveries of 102–115 % for MeHg, while the uptake of Hg(II) was insignificant. The proposed approach was successfully employed for in situ measurements in the Negro River (Manaus-AM, Brazil). - Highlights: • A method for in situ selective determination of MeHg by DGT technique is proposed. • Saccharomyces cerevisiae immobilized in agarose gel was used as binding agent. • Effects of pH, ionic strength and concomitant ions on uptake of MeHg were evaluated. • DGT device containing polyacrylamide gel as diffusive layer showed better selectivity. • The proposed approach was successfully applied for analysis of river water.

  19. EXAMINATION OF THE SURFACE FREE ENERGY AND ACID-BASE PROPERTIES OF CELLULOSE BY THE COLUMN WICKING TECHNIQUE AND THE CRITICAL PACKING HEIGHT/DENSITY

    Institute of Scientific and Technical Information of China (English)

    Qing Shen; Jian-feng Hu; Qing-feng Gu

    2004-01-01

    The column wicking technique was applied to estimate the surface free energy of cellulose, the importance of which is to obtain a real effective capillary radius, Reff, initially from the plot of Washburn penetration distance versus time.Since the cellulose sample could not be packed with good reproducibility, therefore, Reff can not be obtained readily from the slope of the plot. A method was developed in this paper by uniting all apparent packing heights with a unique value to deduce a real effective capillary radius. Based on the defined critical packing height related to the critical packing density, the surface free energy and acid-base properties of cellulose Sigma C8002 were estimated.

  20. Density measurement of thin layers by electron energy loss spectroscopy (EELS).

    Science.gov (United States)

    Thomas, Jürgen; Ramm, Jürgen; Gemming, Thomas

    2013-07-01

    A method to measure the density of thin layers is presented which utilizes electron energy loss spectroscopy (EELS) techniques within a transmission electron microscope. The method is based on the acquisition of energy filtered images in the low loss region as well as of an element distribution map using core loss edges. After correction of multiple inelastic scattering effects, the intensity of the element distribution map is proportional to density and thickness. The dependence of the intensities of images with low energy loss electrons on the density is different from that. This difference allows the calculation of the relative density pixel by pixel and to determine lateral density gradients or fluctuations in thin films without relying on a constant specimen thickness. The method is demonstrated at thin carbon layers produced with density gradients.

  1. Predator Presence and Vegetation Density Affect Capture Rates and Detectability of Litoria aurea Tadpoles: Wide-Ranging Implications for a Common Survey Technique.

    Science.gov (United States)

    Sanders, Madeleine R; Clulow, Simon; Bower, Deborah S; Clulow, John; Mahony, Michael J

    2015-01-01

    Trapping is a common sampling technique used to estimate fundamental population metrics of animal species such as abundance, survival and distribution. However, capture success for any trapping method can be heavily influenced by individuals' behavioural plasticity, which in turn affects the accuracy of any population estimates derived from the data. Funnel trapping is one of the most common methods for sampling aquatic vertebrates, although, apart from fish studies, almost nothing is known about the effects of behavioural plasticity on trapping success. We used a full factorial experiment to investigate the effects that two common environmental parameters (predator presence and vegetation density) have on the trapping success of tadpoles. We estimated that the odds of tadpoles being captured in traps was 4.3 times higher when predators were absent compared to present and 2.1 times higher when vegetation density was high compared to low, using odds ratios based on fitted model means. The odds of tadpoles being detected in traps were also 2.9 times higher in predator-free environments. These results indicate that common environmental factors can trigger behavioural plasticity in tadpoles that biases trapping success. We issue a warning to researchers and surveyors that trapping biases may be commonplace when conducting surveys such as these, and urge caution in interpreting data without consideration of important environmental factors present in the study system. Left unconsidered, trapping biases in capture success have the potential to lead to incorrect interpretations of data sets, and misdirection of limited resources for managing species.

  2. Gradients are shaping up.

    Science.gov (United States)

    Bollenbach, Tobias; Heisenberg, Carl-Philipp

    2015-04-23

    In animal embryos, morphogen gradients determine tissue patterning and morphogenesis. Shyer et al. provide evidence that, during vertebrate gut formation, tissue folding generates graded activity of signals required for subsequent steps of gut growth and differentiation, thereby revealing an intriguing link between tissue morphogenesis and morphogen gradient formation.

  3. Speciation analysis of inorganic arsenic in river water by Amberlite IRA 910 resin immobilized in a polyacrylamide gel as a selective binding agent for As(V) in diffusive gradient thin film technique.

    Science.gov (United States)

    Rolisola, Ana M C M; Suárez, Carlos A; Menegário, Amauri A; Gastmans, Didier; Kiang, Chang H; Colaço, Camila D; Garcez, Daniel L; Santelli, Ricardo E

    2014-09-07

    In this study, a method is proposed for the selective retention of As(V) using diffusive gradient in thin film (DGT) samplers containing a strongly basic anion exchange resin (Amberlite IRA 910) supported on a polyacrylamide gel. In addition, the total arsenic content is determined by ferrihydrite gel discs. Subsequently, the concentration of As(III) was obtained by determining the difference between the total As and As(V). DGT experiments showed linear accumulation of As(V) (up to 280 ng) until a deployment time of 8 h deployment (R(2) > 0.99). The retention of As(V) was appropriate (97.9-112.3%) between pH 5 and 9. For a solution with an ionic strength ranging from 0.001 to 0.05 mol L(-1), the As(V) uptake ranged from 90-120%. The proposed method was applied for the speciation of arsenic in river water. For the analysis of spiked samples collected at the Furnas stream, the recoveries of total arsenic content ranged between 103.9% and 118.8%. However, the recoveries of As(III) and As(V) were 43.3-75.2% and 147.3-153.4%, respectively. These differences were probably because of the oxidation of As(III) to As(V) during deployments. For spiked samples collected at the Ribeirão Claro, the recoveries of dissolved As(III), As(V) and As(T) were 103.1%, 108.0% and 106.3%, respectively. Thus, the DGT technique with Amberlite IRA 910 resin as the binding phase can be employed for the in situ redox speciation of inorganic arsenic.

  4. Fast Distributed Gradient Methods

    CERN Document Server

    Jakovetic, Dusan; Moura, Jose M F

    2011-01-01

    The paper proposes new fast distributed optimization gradient methods and proves convergence to the exact solution at rate O(\\log k/k), much faster than existing distributed optimization (sub)gradient methods with convergence O(1/\\sqrt{k}), while incurring practically no additional communication nor computation cost overhead per iteration. We achieve this for convex (with at least one strongly convex,) coercive, three times differentiable and with Lipschitz continuous first derivative (private) cost functions. Our work recovers for distributed optimization similar convergence rate gains obtained by centralized Nesterov gradient and fast iterative shrinkage-thresholding algorithm (FISTA) methods over ordinary centralized gradient methods. We also present a constant step size distributed fast gradient algorithm for composite non-differentiable costs. A simulation illustrates the effectiveness of our distributed methods.

  5. Polarization Gradient Cooling by Zeeman-Effect-Assisted Saturated Absorption

    Institute of Scientific and Technical Information of China (English)

    HAN Shun-Li; CHENG Bing; ZHANG Jiag-Fang; XU Yun-Fei; WANG Zhao-Ying; LIN Qiang

    2009-01-01

    A novel and simple method to realize polarization gradient cooling(PGC)is reported.The stabilizing,shifting and rapid tuning of the frequency of the external cavity diode laser is realized by using the Zeeman-effect-assisted Doppler-free saturated absorption technique.Based on this convenient technique,~(87)Rb cold atoms are captured from room-temperature background vapor in the magneto-optical trap(MOT).Meanwhile,the steady-state number,the density and the lifetime of atoms in the MOT are measured.Subsequently,a frequency-fast-varying circuit is designed to realize PGC,which is demonstrated effectively and reliably in experiments.The temperature of the cold atom cloud is measured by two different methods,which coincide with each other.

  6. Behavior of the thermal diffusivity of native and oxidized human low-density lipoprotein solutions studied by the Z-scan technique

    Science.gov (United States)

    Santos, Priscila R.; Genaro-Mattos, Thiago C.; Monteiro, Andrea M.; Miyamoto, Sayuri; Figueiredo Neto, Antonio M.

    2012-10-01

    Modifications in low-density lipoprotein (LDL) have emerged as a major pathogenic factor of atherosclerosis, which is the main cause of morbidity and mortality in the western world. Measurements of the heat diffusivity of human LDL solutions in their native and in vitro oxidized states are presented by using the Z-Scan (ZS) technique. Other complementary techniques were used to obtain the physical parameters necessary to interpret the optical results, e.g., pycnometry, refractometry, calorimetry, and spectrophotometry, and to understand the oxidation phase of LDL particles. To determine the sample's thermal diffusivity using the thermal lens model, an iterative one-parameter fitting method is proposed which takes into account several characteristic ZS time-dependent and the position-dependent transmittance measurements. Results show that the thermal diffusivity increases as a function of the LDL oxidation degree, which can be explained by the increase of the hydroperoxides production due to the oxidation process. The oxidation products go from one LDL to another, disseminating the oxidation process and caring the heat across the sample. This phenomenon leads to a quick thermal homogenization of the sample, avoiding the formation of the thermal lens in highly oxidized LDL solutions.

  7. Behavior of the thermal diffusivity of native and oxidized human low-density lipoprotein solutions studied by the Z-scan technique.

    Science.gov (United States)

    Santos, Priscila R; Genaro-Mattos, Thiago C; Monteiro, Andrea M; Miyamoto, Sayuri; Figueiredo Neto, Antonio M

    2012-10-01

    Modifications in low-density lipoprotein (LDL) have emerged as a major pathogenic factor of atherosclerosis, which is the main cause of morbidity and mortality in the western world. Measurements of the heat diffusivity of human LDL solutions in their native and in vitro oxidized states are presented by using the Z-Scan (ZS) technique. Other complementary techniques were used to obtain the physical parameters necessary to interpret the optical results, e.g., pycnometry, refractometry, calorimetry, and spectrophotometry, and to understand the oxidation phase of LDL particles. To determine the sample's thermal diffusivity using the thermal lens model, an iterative one-parameter fitting method is proposed which takes into account several characteristic ZS time-dependent and the position-dependent transmittance measurements. Results show that the thermal diffusivity increases as a function of the LDL oxidation degree, which can be explained by the increase of the hydroperoxides production due to the oxidation process. The oxidation products go from one LDL to another, disseminating the oxidation process and caring the heat across the sample. This phenomenon leads to a quick thermal homogenization of the sample, avoiding the formation of the thermal lens in highly oxidized LDL solutions.

  8. EVALUATION OF BONE-TO-IMPLANT CONTACT AND BONE DENSITY ADJACENT TO TITANIUM IMPLANTS USING A STEREOLOGICAL TECHNIQUE ON GROUND SECTIONS

    Directory of Open Access Journals (Sweden)

    Dimitra Balatsouka

    2011-05-01

    Full Text Available When bone implants have to be examined in situ ground sections are required. Histomorphometric measurements are usually performed on two-dimensional sections, causing biased results when they are wrongly extrapolated to 3D without any knowledge of stereology. Unbiased results can only be obtained using stereological principles. The aim of the study was to describe an unbiased design for evaluating boneto-mplant contact (BIC and peri-implant bone density (BD-i in three-dimensions. The unbiased design was based on a fixed axis vertical random sampling technique. Three bone-implant blocks were collected from 3 rabbits. Four sections were obtained from each animal using a fixed axis vertical random sampling technique. The BIC was estimated by creating a stereological method based on a systematic test line set. The BD-i was estimated using a design based on a systematic point set. The efficiency of the systematic sampling was evaluated for each sampling level: Coefficient of error CE( i w for the systematic test line set, CESyst between the 4 rotated sections, CENoise for the reproducibility. These variances were compared to the biological variation (CV between animals. The mean CE( i w was 5 to 6%; the CESyst was 7.8% for the BIC estimates and 5.7% for the BD-i estimates; the CENoise was 5.8% for the BIC and 7.7% for the BD-i. The CV was 19% for the BIC estimates and 24% for the BD-i estimates. These results demonstrated that the stereological technique used in the present study was a very efficient method to obtain unbiased estimates of BIC and BD-i in 3D on 2D implant-bone sections.

  9. Large-scale symmetry-adapted perturbation theory computations via density fitting and Laplace transformation techniques: Investigating the fundamental forces of DNA-intercalator interactions

    Science.gov (United States)

    Hohenstein, Edward G.; Parrish, Robert M.; Sherrill, C. David; Turney, Justin M.; Schaefer, Henry F.

    2011-11-01

    Symmetry-adapted perturbation theory (SAPT) provides a means of probing the fundamental nature of intermolecular interactions. Low-orders of SAPT (here, SAPT0) are especially attractive since they provide qualitative (sometimes quantitative) results while remaining tractable for large systems. The application of density fitting and Laplace transformation techniques to SAPT0 can significantly reduce the expense associated with these computations and make even larger systems accessible. We present new factorizations of the SAPT0 equations with density-fitted two-electron integrals and the first application of Laplace transformations of energy denominators to SAPT. The improved scalability of the DF-SAPT0 implementation allows it to be applied to systems with more than 200 atoms and 2800 basis functions. The Laplace-transformed energy denominators are compared to analogous partial Cholesky decompositions of the energy denominator tensor. Application of our new DF-SAPT0 program to the intercalation of DNA by proflavine has allowed us to determine the nature of the proflavine-DNA interaction. Overall, the proflavine-DNA interaction contains important contributions from both electrostatics and dispersion. The energetics of the intercalator interaction are are dominated by the stacking interactions (two-thirds of the total), but contain important contributions from the intercalator-backbone interactions. It is hypothesized that the geometry of the complex will be determined by the interactions of the intercalator with the backbone, because by shifting toward one side of the backbone, the intercalator can form two long hydrogen-bonding type interactions. The long-range interactions between the intercalator and the next-nearest base pairs appear to be negligible, justifying the use of truncated DNA models in computational studies of intercalation interaction energies.

  10. Second gradient poromechanics

    CERN Document Server

    Sciarra, Giulio; Coussy, Olivier

    2010-01-01

    Second gradient theories have been developed in mechanics for treating different phenomena as capillarity in fluids, plasticity and friction in granular materials or shear band deformations. Here, there is an attempt of formulating a second gradient Biot like model for porous materials. In particular the interest is focused in describing the local dilatant behaviour of a porous material induced by pore opening elastic and capillary interaction phenomena among neighbouring pores and related micro-filtration phenomena by means of a continuum microstructured model. The main idea is to extend the classical macroscopic Biot model by including in the description second gradient effects. This is done by assuming that the surface contribution to the external work rate functional depends on the normal derivative of the velocity or equivalently assuming that the strain work rate functional depends on the porosity and strain gradients. According to classical thermodynamics suitable restrictions for stresses and second g...

  11. Improving the accuracy of pulsed field gradient NMR diffusion experiments: Correction for gradient non-uniformity

    Science.gov (United States)

    Connell, Mark A.; Bowyer, Paul J.; Adam Bone, P.; Davis, Adrian L.; Swanson, Alistair G.; Nilsson, Mathias; Morris, Gareth A.

    2009-05-01

    Pulsed field gradient NMR is a well-established technique for the determination of self-diffusion coefficients. However, a significant source of systematic error exists in the spatial variation of the applied pulsed field gradient. Non-uniform pulsed field gradients cause the decay of peak amplitudes to deviate from the expected exponential dependence on gradient squared. This has two undesirable effects: the apparent diffusion coefficient will deviate from the true value to an extent determined by the choice of experimental parameters, and the error estimated by the nonlinear least squares fitting will contain a significant systematic contribution. In particular, the apparent diffusion coefficient determined by exponential fitting of the diffusional attenuation of NMR signals will depend both on the exact pulse widths used and on the range of gradient amplitudes chosen. These problems can be partially compensated for if experimental attenuation data are fitted to a function corrected for the measured spatial dependence of the gradient and signal strength. This study describes a general alternative to existing methods for the calibration of NMR diffusion measurements. The dominant longitudinal variation of the pulsed field gradient amplitude and the signal strength are mapped by measuring pulsed field gradient echoes in the presence of a weak read gradient. These data are then used to construct a predicted signal decay function for the whole sample, which is parameterised as the exponential of a power series. Results are presented which compare diffusion coefficients obtained using the new calibration method with previous literature values.

  12. Design of spherical symmetric gradient index lenses

    Science.gov (United States)

    Miñano, Juan C.; Grabovičkić, Dejan; Benítez, Pablo; González, Juan C.; Santamaría, Asunción

    2012-10-01

    Spherical symmetric refractive index distributions also known as Gradient Index lenses such as the Maxwell-Fish-Eye (MFE), the Luneburg or the Eaton lenses have always played an important role in Optics. The recent development of the technique called Transformation Optics has renewed the interest in these gradient index lenses. For instance, Perfect Imaging within the Wave Optics framework has recently been proved using the MFE distribution. We review here the design problem of these lenses, classify them in two groups (Luneburg moveable-limits and fixed-limits type), and establish a new design techniques for each type of problem.

  13. Cell orientation gradients on an inverse opal substrate.

    Science.gov (United States)

    Lu, Jie; Zou, Xin; Zhao, Ze; Mu, Zhongde; Zhao, Yuanjin; Gu, Zhongze

    2015-05-20

    The generation of cell gradients is critical for understanding many biological systems and realizing the unique functionality of many implanted biomaterials. However, most previous work can only control the gradient of cell density and this has no effect on the gradient of cell orientation, which has an important role in regulating the functions of many connecting tissues. Here, we report on a simple stretched inverse opal substrate for establishing desired cell orientation gradients. It was demonstrated that tendon fibroblasts on the stretched inverse opal gradient showed a corresponding alignment along with the elongation gradient of the substrate. This "random-to-aligned" cell gradient reproduces the insertion part of many connecting tissues, and thus, will have important applications in tissue engineering.

  14. Critical current density and ac harmonic voltage generation in YBa{sub 2}Cu{sub 3}O{sub 7-{delta}} thin films by the screening technique

    Energy Technology Data Exchange (ETDEWEB)

    Perez-Lopez, Israel O., E-mail: iperez@mda.cinvestav.m [Applied Physics Department, Cinvestav Unidad Merida, Km 6 Ant., Carretera a Progreso, A.P. 73, C.P. 97310 Merida, Yucatan (Mexico); Gamboa, Fidel; Sosa, Victor [Applied Physics Department, Cinvestav Unidad Merida, Km 6 Ant., Carretera a Progreso, A.P. 73, C.P. 97310 Merida, Yucatan (Mexico)

    2010-12-15

    The temperature and field dependence of harmonics in voltage V{sub n}=V{sub n}{sup '}-iV{sub n}{sup ''} using the screening technique have been measured for YBa{sub 2}Cu{sub 3}O{sub 7-{delta}} superconducting thin films. Using the Sun model we obtained the curves for the temperature-dependent critical current density J{sub c}(T). In addition, we applied the criterion proposed by Acosta et al. to compute J{sub c}(T). Also, we made used of the empirical law J{sub c{proportional_to}}(1-T/T{sub c}){sup n} as an input in our calculations to reproduce experimental harmonic generation up to the fifth harmonic. We found that most models fit well the fundamental voltage but higher harmonics are poorly reproduced. Such behavior suggests the idea that higher harmonics contain information concerning complex processes like flux creep or thermally assisted flux flow.

  15. The Number Density Evolution of Extreme Emission Line Galaxies in 3D-HST: Results from a Novel Automated Line Search Technique for Slitless Spectroscopy

    CERN Document Server

    Maseda, Michael V; Rix, Hans-Walter; Momcheva, Ivelina; Brammer, Gabriel B; Franx, Marijn; Lundgren, Britt F; Skelton, Rosalind E; Whitaker, Katherine E

    2016-01-01

    The multiplexing capability of slitless spectroscopy is a powerful asset in creating large spectroscopic datasets, but issues such as spectral confusion make the interpretation of the data challenging. Here we present a new method to search for emission lines in the slitless spectroscopic data from the 3D-HST survey utilizing the Wide-Field Camera 3 on board the Hubble Space Telescope. Using a novel statistical technique, we can detect compact (extended) emission lines at 90% completeness down to fluxes of 1.5 (3.0) times 10^{-17} erg/s/cm^2, close to the noise level of the grism exposures, for objects detected in the deep ancillary photometric data. Unlike previous methods, the Bayesian nature allows for probabilistic line identifications, namely redshift estimates, based on secondary emission line detections and/or photometric redshift priors. As a first application, we measure the comoving number density of Extreme Emission Line Galaxies (restframe [O III] 5007 equivalent widths in excess of 500 Angstroms)...

  16. High Yield Cultivation Techniques of Density-tolerant Spring Maize in Northern China%北方耐密春玉米高产栽培技术

    Institute of Scientific and Technical Information of China (English)

    杨长海

    2014-01-01

    Based on the introduction of maize planting technology, the yield increasing effect and efficiency principle, the author gives a detailed description of the cultivation techniques of density-tolerant maize in northern China from plot election and soil preparation, seed treatment, variety selection, fertilization, pest and disease control in an effort to provide a theoretical reference for increasing the yield of Spring Maize in Northern China.%在概述玉米密植技术的增产效果及增产增效原理的基础上,从选地和整地、品种选择、种子处理、施肥、病虫害防治5方面,详细介绍北方地区耐密玉米高产栽培技术要点,为提高北方地区春玉米产量提供理论参考。

  17. A Wavelet-Based Assessment of Topographic-Isostatic Reductions for GOCE Gravity Gradients

    Science.gov (United States)

    Grombein, Thomas; Luo, Xiaoguang; Seitz, Kurt; Heck, Bernhard

    2014-07-01

    Gravity gradient measurements from ESA's satellite mission Gravity field and steady-state Ocean Circulation Explorer (GOCE) contain significant high- and mid-frequency signal components, which are primarily caused by the attraction of the Earth's topographic and isostatic masses. In order to mitigate the resulting numerical instability of a harmonic downward continuation, the observed gradients can be smoothed with respect to topographic-isostatic effects using a remove-compute-restore technique. For this reason, topographic-isostatic reductions are calculated by forward modeling that employs the advanced Rock-Water-Ice methodology. The basis of this approach is a three-layer decomposition of the topography with variable density values and a modified Airy-Heiskanen isostatic concept incorporating a depth model of the Mohorovičić discontinuity. Moreover, tesseroid bodies are utilized for mass discretization and arranged on an ellipsoidal reference surface. To evaluate the degree of smoothing via topographic-isostatic reduction of GOCE gravity gradients, a wavelet-based assessment is presented in this paper and compared with statistical inferences in the space domain. Using the Morlet wavelet, continuous wavelet transforms are applied to measured GOCE gravity gradients before and after reducing topographic-isostatic signals. By analyzing a representative data set in the Himalayan region, an employment of the reductions leads to significantly smoothed gradients. In addition, smoothing effects that are invisible in the space domain can be detected in wavelet scalograms, making a wavelet-based spectral analysis a powerful tool.

  18. Multiscale architectured materials with composition and grain size gradients manufactured using high-pressure torsion.

    Science.gov (United States)

    Kang, Ji Yun; Kim, Jung Gi; Park, Hyo Wook; Kim, Hyoung Seop

    2016-05-27

    The concept of multiscale architectured materials is established using composition and grain size gradients. Composition-gradient nanostructured materials are produced from coarse grained interstitial free steels via carburization and high-pressure torsion. Quantitative analyses of the dislocation density using X-ray diffraction and microstructural studies clearly demonstrate the gradients of the dislocation density and grain size. The mechanical properties of the gradient materials are compared with homogeneous nanostructured carbon steel without a composition gradient in an effort to investigate the gradient effect. Based on the above observations, the potential of multiscale architecturing to open a new material property is discussed.

  19. Mass Function Gradients and the Need for Dark Matter

    CERN Document Server

    Taylor, J A

    1998-01-01

    There is substantial evidence that the initial mass function (IMF) may be a function of the local star formation conditions. In particular, the IMF is predicted to flatten with increasing local luminosity density, with the formation of massive stars being preferentially enhanced in brighter regions. If IMF gradients are general features of galaxies, several previous astrophysical measurements, such as the surface mass densities of spirals (obtained assuming constant mass to light ratios), were plagued by substantial systematic errors. In this Letter, calculations which account for possible IMF gradients are presented of surface densities of spiral galaxies. Compared to previous estimates, the mass densities corrected for IMF gradients are higher in the outer regions of the disks. For a model based on the Milky Way but with an IMF scaled according to R136, the rotation curve without the traditional dark halo component falls with galactrocentric radius, though slower than it would without IMF gradients. For a s...

  20. High Gradient Accelerator Research

    Energy Technology Data Exchange (ETDEWEB)

    Temkin, Richard [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Physics. Plasma Science and Fusion Center

    2016-07-12

    The goal of the MIT program of research on high gradient acceleration is the development of advanced acceleration concepts that lead to a practical and affordable next generation linear collider at the TeV energy level. Other applications, which are more near-term, include accelerators for materials processing; medicine; defense; mining; security; and inspection. The specific goals of the MIT program are: • Pioneering theoretical research on advanced structures for high gradient acceleration, including photonic structures and metamaterial structures; evaluation of the wakefields in these advanced structures • Experimental research to demonstrate the properties of advanced structures both in low-power microwave cold test and high-power, high-gradient test at megawatt power levels • Experimental research on microwave breakdown at high gradient including studies of breakdown phenomena induced by RF electric fields and RF magnetic fields; development of new diagnostics of the breakdown process • Theoretical research on the physics and engineering features of RF vacuum breakdown • Maintaining and improving the Haimson / MIT 17 GHz accelerator, the highest frequency operational accelerator in the world, a unique facility for accelerator research • Providing the Haimson / MIT 17 GHz accelerator facility as a facility for outside users • Active participation in the US DOE program of High Gradient Collaboration, including joint work with SLAC and with Los Alamos National Laboratory; participation of MIT students in research at the national laboratories • Training the next generation of Ph. D. students in the field of accelerator physics.

  1. Model comparison for the density structure along solar prominence threads

    CERN Document Server

    Arregui, I

    2015-01-01

    Quiescent solar prominence fine structures are typically modelled as density enhancements, called threads, which occupy a fraction of a longer magnetic flux tube. The profile of the mass density along the magnetic field is however unknown and several arbitrary alternatives are employed in prominence wave studies. We present a comparison of theoretical models for the field-aligned density along prominence fine structures. We consider Lorentzian, Gaussian, and parabolic profiles. We compare their theoretical predictions for the period ratio between the fundamental transverse kink mode and the first overtone to obtain estimates for the ratio of densities between the central part of the tube and its foot-points and to assess which one would better explain observed period ratio data. Bayesian parameter inference and model comparison techniques are developed and applied. Parameter inference requires the computation of the posterior distribution for the density gradient parameter conditional on the observable period...

  2. Uniform gradient expansions

    Directory of Open Access Journals (Sweden)

    Massimo Giovannini

    2015-06-01

    Full Text Available Cosmological singularities are often discussed by means of a gradient expansion that can also describe, during a quasi-de Sitter phase, the progressive suppression of curvature inhomogeneities. While the inflationary event horizon is being formed the two mentioned regimes coexist and a uniform expansion can be conceived and applied to the evolution of spatial gradients across the protoinflationary boundary. It is argued that conventional arguments addressing the preinflationary initial conditions are necessary but generally not sufficient to guarantee a homogeneous onset of the conventional inflationary stage.

  3. Uniform gradient expansions

    Energy Technology Data Exchange (ETDEWEB)

    Giovannini, Massimo, E-mail: massimo.giovannini@cern.ch [Department of Physics, Theory Division, CERN, 1211 Geneva 23 (Switzerland); INFN, Section of Milan-Bicocca, 20126 Milan (Italy)

    2015-06-30

    Cosmological singularities are often discussed by means of a gradient expansion that can also describe, during a quasi-de Sitter phase, the progressive suppression of curvature inhomogeneities. While the inflationary event horizon is being formed the two mentioned regimes coexist and a uniform expansion can be conceived and applied to the evolution of spatial gradients across the protoinflationary boundary. It is argued that conventional arguments addressing the preinflationary initial conditions are necessary but generally not sufficient to guarantee a homogeneous onset of the conventional inflationary stage.

  4. Impact toughness of a gradient hardened layer of Cr5Mo1V steel treated by laser shock peening

    Science.gov (United States)

    Xia, Weiguang; Li, Lei; Wei, Yanpeng; Zhao, Aimin; Guo, Yacong; Huang, Chenguang; Yin, Hongxiang; Zhang, Lingchen

    2016-04-01

    Laser shock peening (LSP) is a widely used surface treatment technique that can effectively improve the fatigue life and impact toughness of metal parts. Cr5Mo1V steel exhibits a gradient hardened layer after a LSP process. A new method is proposed to estimate the impact toughness that considers the changing mechanical properties in the gradient hardened layer. Assuming a linearly gradient distribution of impact toughness, the parameters controlling the impact toughness of the gradient hardened layer were given. The influences of laser power densities and the number of laser shots on the impact toughness were investigated. The impact toughness of the laser peened layer improves compared with an untreated specimen, and the impact toughness increases with the laser power densities and decreases with the number of laser shots. Through the fracture morphology analysis by a scanning electron microscope, we established that the Cr5Mo1V steel was fractured by the cleavage fracture mechanism combined with a few dimples. The increase in the impact toughness of the material after LSP is observed because of the decreased dimension and increased fraction of the cleavage fracture in the gradient hardened layer.

  5. Gradient systems and mechanical systems

    Institute of Scientific and Technical Information of China (English)

    Fengxiang Mei; Huibin Wu

    2016-01-01

    All types of gradient systems and their properties are discussed. Two problems connected with gradient sys-tems and mechanical systems are studied. One is the direct problem of transforming a mechanical system into a gradi-ent system, and the other is the inverse problem, which is transforming a gradient system into a mechanical system.

  6. Collisional transport in a plasma with steep gradients

    Energy Technology Data Exchange (ETDEWEB)

    Wang, W.; Okamoto, M.; Nakajima, N.; Murakami, S. [National Inst. for Fusion Science, Toki, Gifu (Japan)

    1999-06-01

    The validity is given to the newly proposed two {delta}f method for neoclassical transport calculation, which can be solve the drift kinetic equation considering effects of steep plasma gradients, large radial electric field, finite banana width, and an orbit topology near the axis. The new method is applied to the study of ion transport with steep plasma gradients. It is found that the ion thermal diffusivity decreases as the scale length of density gradient decreases, while the ion particle flux due to ion-ion self collisions increases with increasing gradient. (author)

  7. Measurement of induced magnetic flux density using injection current nonlinear encoding (ICNE) in MREIT.

    Science.gov (United States)

    Park, Chunjae; Lee, Byung Il; Kwon, Ohin; Woo, Eung Je

    2007-02-01

    Magnetic resonance electrical impedance tomography (MREIT) measures induced magnetic flux densities subject to externally injected currents in order to visualize conductivity distributions inside an electrically conducting object. Injection currents induce magnetic flux densities that appear in phase parts of acquired MR image data. In the conventional current injection method, we inject currents during the time segment between the end of the first RF pulse and the beginning of the reading gradient in order to ensure the gradient linearity. Noting that longer current injections can accumulate more phase changes, we propose a new pulse sequence called injection current nonlinear encoding (ICNE) where the duration of the injection current pulse is extended until the end of the reading gradient. Since the current injection during the reading gradient disturbs the gradient linearity, we first analyze the MR signal produced by the ICNE pulse sequence and suggest a novel algorithm to extract the induced magnetic flux density from the acquired MR signal. Numerical simulations and phantom experiments show that the new method is clearly advantageous in terms of the reduced noise level in measured magnetic flux density data. The amount of noise reduction depends on the choice of the data acquisition time and it was about 24% when we used a prolonged data acquisition time of 10.8 ms. The ICNE method will enhance the clinical applicability of the MREIT technique when it is combined with an appropriate phase artefact minimization method.

  8. Time Rate Gradient Effects and Negative Mass

    Science.gov (United States)

    Miksch, Edmond

    2008-03-01

    The Harvard tower Experiment and tests with accurate atomic clocks show that a clock at a high elevation indicates more elapsed time than a clock at a low elevation, both clocks properly measuring time at their locations. This fact mandates that Newton's first law of motion be rewritten to cite impulse balance rather than force balance. Time rate gradient effects explain how the weight of a precisely vertical and precisely uniform electric field or a precisely vertical and precisely uniform magnetic field is supported in a precisely unidirectional gravitational field. Time rate gradient effects also explain how the weight of a unidirectional gravitational field is reacted. It is confirmed that the mass density of the gravitational field is negative. http://www.TimeRateGradient.com; http://www.Negative-Mass.com; http://www.EinsteinsElevator.com

  9. 应用密度梯度离心和贴壁筛选法分离免骨髓间充质干细胞%Isolation of rabbit bone marrow mesenchymal stem cells using density gradient centrifugation and adherence screening methods

    Institute of Scientific and Technical Information of China (English)

    张才龙; 夏长所; 蒋正尧

    2009-01-01

    BACKGROUND: Under special conditions, bone marrow mesenchymal stem cells (BMSCs) can differentiate into osteoblasts and chondroblasts. However, MSCs are few in bone marrow. How to harvest, purity and rapidly proliferate in vitro is a foundation of application in tissue engineering technique. OBJECTIVE: To optimize, collect, purity, assess rabbit BMSCs and to observe the biological character of BMSCs. DESIGN, TIME AND SETTING: The observational study was performed at the Animal Experimental Center of Tongji Medical College from September 2005 to July 2006. MATERIALS: One female New Zealand rabbits aged 2 months were used for MSC collection and primary culture. METHODS: Bone marrow solution was purified by density gradient centrifugation and adherence screening method. Culture solution was obtained. BMSCs were incubated in phosphate buffered solution (PBS), supplemented with 2.5 g/L trypsin (3.0 mL), and placed in an incubator at 37 ℃ for two or three minutes. Cell morphology was observed using an inverted microscope. The digestion was stopped when cytoplasm recovery, long and thin cells with large intercellular space, and few round cells appeared. Subsequently, BMSCs were incubated in serum-free L-DMEM, and placed in a plastic culture flask at 1.0×108/L. MAIN OUTCOME MEASURES: MSC morphology, ultrastructura and surface marker; Proliferation of the first, third, fifth, eighth and tenth passages of BMSCs; Cell growth curve was drawn. RESULTS: BMSCs was pure following density gradient centrifugation and adherence screening method. The third and fifth passage of cells had typical whirlpool-shape. Transmission electron microscope demonstrated that round or oval MSCs possessed large nuclei, big nucleus proportion, a few cellular organ. These were low-differentiated cells. Growth curve of cultured MSCs was "S" shape. The first, third and fifth passage cells had strong reproductive capability. The eighth and tenth passage of cells had significantly reduced proliferation

  10. Sorting cells by their density

    Science.gov (United States)

    Norouzi, Nazila; Bhakta, Heran C.

    2017-01-01

    Sorting cells by their type is an important capability in biological research and medical diagnostics. However, most cell sorting techniques rely on labels or tags, which may have limited availability and specificity. Sorting different cell types by their different physical properties is an attractive alternative to labels because all cells intrinsically have these physical properties. But some physical properties, like cell size, vary significantly from cell to cell within a cell type; this makes it difficult to identify and sort cells based on their sizes alone. In this work we continuously sort different cells types by their density, a physical property with much lower cell-to-cell variation within a cell type (and therefore greater potential to discriminate different cell types) than other physical properties. We accomplish this using a 3D-printed microfluidic chip containing a horizontal flowing micron-scale density gradient. As cells flow through the chip, Earth’s gravity makes each cell move vertically to the point where the cell’s density matches the surrounding fluid’s density. When the horizontal channel then splits, cells with different densities are routed to different outlets. As a proof of concept, we use our density sorter chip to sort polymer microbeads by their material (polyethylene and polystyrene) and blood cells by their type (white blood cells and red blood cells). The chip enriches the fraction of white blood cells in a blood sample from 0.1% (in whole blood) to nearly 98% (in the output of the chip), a 1000x enrichment. Any researcher with access to a 3D printer can easily replicate our density sorter chip and use it in their own research using the design files provided as online Supporting Information. Additionally, researchers can simulate the performance of a density sorter chip in their own applications using the Python-based simulation software that accompanies this work. The simplicity, resolution, and throughput of this

  11. A comparison of conventional maximum intensity projection with a new depth-specific topographic mapping technique in the CT analysis of proximal tibial subchondral bone density

    Energy Technology Data Exchange (ETDEWEB)

    Johnston, James D. [University of Saskatchewan, Department of Mechanical Engineering, Saskatoon, SK (Canada); University of British Columbia, Department of Mechanical Engineering, Vancouver, BC (Canada); Kontulainen, Saija A. [University of Saskatchewan, College of Kinesiology, Saskatoon, SK (Canada); Masri, Bassam A.; Wilson, David R. [University of British Columbia, Department of Orthopaedics, Vancouver, BC (Canada)

    2010-09-15

    The objective was to identify subchondral bone density differences between normal and osteoarthritic (OA) proximal tibiae using computed tomography osteoabsorptiometry (CT-OAM) and computed tomography topographic mapping of subchondral density (CT-TOMASD). Sixteen intact cadaver knees from ten donors (8 male:2 female; mean age:77.8, SD:7.4 years) were categorized as normal (n = 10) or OA (n = 6) based upon CT reconstructions. CT-OAM assessed maximum subchondral bone mineral density (BMD). CT-TOMASD assessed average subchondral BMD across three layers (0-2.5, 2.5-5 and 5-10 mm) measured in relation to depth from the subchondral surface. Regional analyses of CT-OAM and CT-TOMASD included: medial BMD, lateral BMD, and average BMD of a 10-mm diameter area that searched each medial and lateral plateau for the highest ''focal'' density present within each knee. Compared with normal knees, both CT-OAM and CT-TOMASD demonstrated an average of 17% greater whole medial compartment density in OA knees (p < 0.016). CT-OAM did not distinguish focal density differences between OA and normal knees (p > 0.05). CT-TOMASD focal region analyses revealed an average of 24% greater density in the 0- to 2.5-mm layer (p = 0.003) and 36% greater density in the 2.5- to 5-mm layer (p = 0.034) in OA knees. Both CT-OAM and TOMASD identified higher medial compartment density in OA tibiae compared with normal tibiae. In addition, CT-TOMASD indicated greater focal density differences between normal and OA knees with increased depth from the subchondral surface. Depth-specific density analyses may help identify and quantify small changes in subchondral BMD associated with OA disease onset and progression. (orig.)

  12. Dropwise Condensation on a Radial Gradient Surface

    Science.gov (United States)

    Macner, Ashley; Daniel, Susan; Steen, Paul

    2013-11-01

    In transient dropwise condensation from steam onto a cool surface, distributions of drops evolve by nucleation, growth, and coalescence. This study examines how surface functionalization affects drop growth and coalescence. Surfaces are treated by silanization to deliver either a spatially uniform contact-angle (hydrophilic, neutral, and hydrophobic) or a radial gradient of contact-angles. The time evolution of number-density and associated drop-size distributions are reported. For a typical condensation experiment on a uniform angle surface, the number-density curves show two regimes: an initial increase in number-density as a result of nucleation and a subsequent decrease in number-density as a result of larger scale coalescence events. Without a removal mechanism, the fractional coverage, regardless of treatment, approaches unity. For the same angle-surface, the associated drop-size distributions progress through four different shapes along the growth curve. In contrast, for a radial gradient surface where removal by sweeping occurs, the number-density increases and then levels off to a value close to the maximum number-density that is well below unity coverage and only two shapes of distributions are observed. Implications for heat transfer will be discussed. This work was supported by a NASA Office of the Chief Technologist's Space Technology Research Fellowship.

  13. Gradient Bundle Analysis: A Full Topological Approach to Chemical Bonding

    CERN Document Server

    Morgenstern, Amanda

    2016-01-01

    The "chemical bond" is a central concept in molecular sciences, but there is no consensus as to what a bond actually is. Therefore, a variety of bonding models have been developed, each defining the structure of molecules in a different manner with the goal of explaining and predicting chemical properties. This thesis describes the initial development of gradient bundle analysis (GBA), a chemical bonding model that creates a high resolution picture of chemical interactions within the charge density framework. GBA is based on concepts from the quantum theory of atoms in molecules (QTAIM), but uses a more complete picture of the topology and geometry of the electron charge density to understand and predict bonding interactions. Gradient bundles are defined as volumes bounded by zero-flux surfaces (ZFSs) in the gradient of the charge density with well-defined energies. The structure of gradient bundles provides an avenue for detecting the locations of valence electrons, which correspond to reactive regions in a ...

  14. NIF optics phase gradient specfication

    Energy Technology Data Exchange (ETDEWEB)

    Williams, W.; Auerbach, J.; Hunt, J.; Lawson, L.; Manes, K.; Orth, C.; Sacks, R.; Trenholme, J.; Wegner, P.

    1997-05-02

    A root-mean-square (rms) phase gradient specification seems to allow a good connection between the NIP optics quality and focal spot requirements. Measurements on Beamlet optics individually, and as a chain, indicate they meet the assumptions necessary to use this specification, and that they have a typical rms phase gradient of {approximately}80 {angstrom}/cm. This may be sufficient for NIP to meet the proposed Stockpile Stewardship Management Program (SSMP) requirements of 80% of a high- power beam within a 200-250 micron diameter spot. Uncertainties include, especially, the scale length of the optics phase noise, the ability of the adaptive optic to correct against pump-induced distortions and optics noise, and the possibility of finding mitigation techniques against whole-beam self-focusing (e.g. a pre- correction optic). Further work is needed in these areas to better determine the NIF specifications. This memo is a written summary of a presentation on this topic given by W. Williams 24 April 1997 to NIP and LS&T personnel.

  15. Constrained length minimum inductance gradient coil design.

    Science.gov (United States)

    Chronik, B A; Rutt, B K

    1998-02-01

    A gradient coil design algorithm capable of controlling the position of the homogeneous region of interest (ROI) with respect to the current-carrying wires is required for many advanced imaging and spectroscopy applications. A modified minimum inductance target field method that allows the placement of a set of constraints on the final current density is presented. This constrained current minimum inductance method is derived in the context of previous target field methods. Complete details are shown and all equations required for implementation of the algorithm are given. The method has been implemented on computer and applied to the design of both a 1:1 aspect ratio (length:diameter) central ROI and a 2:1 aspect ratio edge ROI gradient coil. The 1:1 design demonstrates that a general analytic method can be used to easily obtain very short gradient coil designs for use with specialized magnet systems. The edge gradient design demonstrates that designs that allow imaging of the neck region with a head sized gradient coil can be obtained, as well as other applications requiring edge-of-cylinder regions of uniformity.

  16. Adaptive Regularization of Neural Networks Using Conjugate Gradient

    DEFF Research Database (Denmark)

    Goutte, Cyril; Larsen, Jan

    1998-01-01

    Andersen et al. (1997) and Larsen et al. (1996, 1997) suggested a regularization scheme which iteratively adapts regularization parameters by minimizing validation error using simple gradient descent. In this contribution we present an improved algorithm based on the conjugate gradient technique........ Numerical experiments with feedforward neural networks successfully demonstrate improved generalization ability and lower computational cost...

  17. A NONMONOTONE CONJUGATE GRADIENT ALGORITHM FOR UNCONSTRAINED OPTIMIZATION

    Institute of Scientific and Technical Information of China (English)

    DAI Yuhong

    2002-01-01

    Conjugate gradient methods are very important methods for unconstrained optimization, especially for large scale problems. In this paper, we propose a new conjugate gradient method, in which the technique of nonmonotone line search is used. Under mild assumptions, we prove the global convergence of the method. Some numerical results are also presented.

  18. Bigravity from gradient expansion

    Energy Technology Data Exchange (ETDEWEB)

    Yamashita, Yasuho [Yukawa Institute for Theoretical Physics, Kyoto University,606-8502, Kyoto (Japan); Tanaka, Takahiro [Yukawa Institute for Theoretical Physics, Kyoto University,606-8502, Kyoto (Japan); Department of Physics, Kyoto University,606-8502, Kyoto (Japan)

    2016-05-04

    We discuss how the ghost-free bigravity coupled with a single scalar field can be derived from a braneworld setup. We consider DGP two-brane model without radion stabilization. The bulk configuration is solved for given boundary metrics, and it is substituted back into the action to obtain the effective four-dimensional action. In order to obtain the ghost-free bigravity, we consider the gradient expansion in which the brane separation is supposed to be sufficiently small so that two boundary metrics are almost identical. The obtained effective theory is shown to be ghost free as expected, however, the interaction between two gravitons takes the Fierz-Pauli form at the leading order of the gradient expansion, even though we do not use the approximation of linear perturbation. We also find that the radion remains as a scalar field in the four-dimensional effective theory, but its coupling to the metrics is non-trivial.

  19. Increasing SLEDed Linac Gradient

    Energy Technology Data Exchange (ETDEWEB)

    Farkas, Zoltan D

    2001-11-08

    This note will show how to increase the SLED [1] gradient by varying Q{sub e}, the external Q of the SLED cavity, by increasing its Q{sub 0} and by increasing the compression ratio. If varying the external Q is to be effective, then the copper losses should be small so that Q{sub 0} >> Q{sub e}. Methods of varying Q{sub e} will be indicated but no experimental data will be presented. If we increase the klystron pulse width from 3.5 to 5 {micro}S and increase Q{sub 0} from the present 100000 to 300000, then the gradient increases by 19% and the beam energy increases from 50 to 60 GeV. This note will also discuss SLED operation at 11424 MHz, the NLC frequency. Without Q{sub e} switching, using SLED at 11424 MHz increases the SLAC gradient from 21 MV/m to 34 MV/m, and at the same repetition rate, uses about 1/5 of rf average power. If we also double the compression ratio, we reach 47 MV/m and over 100 GeV beam energy.

  20. Dropwise condensation on a cold gradient substrate

    Science.gov (United States)

    Macner, Ashley; Daniel, Susan; Steen, Paul

    2012-11-01

    Distributions of drops that arise from dropwise condensation evolve by nucleation, growth, and coalescence of drops. An understanding of how surface-energy gradients applied to the substrate affect drop growth and coalescence is needed for design of effective surfaces for large-scale dropwise condensation. Transient dropwise condensation from a vapor phase onto a cold and chemically treated surface is reported. The surfaces were treated to deliver either a uniform contact-angle or a gradient of contact-angles by silanization. The time evolution of drop-size and number-density distributions is reported. For a typical condensation experiment, the drop distributions advance through two stages: an increase in drop density as a result of nucleation and a decrease in drop density as a result of larger scale coalescence events. Because the experiment is transient in nature, the shape of the distribution can be used to predict the number of drop generations and their stage of development. Preliminary results for gradient surfaces will be discussed and compared against observations of behavior on uniformly coated surfaces. NASA Space Technology Research Fellowship (NSTRF).

  1. Gradient induced liquid motion on laser structured black Si surfaces

    Science.gov (United States)

    Paradisanos, I.; Fotakis, C.; Anastasiadis, S. H.; Stratakis, E.

    2015-09-01

    This letter reports on the femtosecond laser fabrication of gradient-wettability micro/nano-patterns on Si surfaces. The dynamics of directional droplet spreading on the surface tension gradients developed is systematically investigated and discussed. It is shown that microdroplets on the patterned surfaces spread at a maximum speed of 505 mm/s, which is the highest velocity demonstrated so far for liquid spreading on a surface tension gradient in ambient conditions. The application of the proposed laser patterning technique for the precise fabrication of surface tension gradients for open microfluidic systems, liquid management in fuel cells, and drug delivery is envisaged.

  2. Gradient induced liquid motion on laser structured black Si surfaces

    CERN Document Server

    Paradisanos, I; Anastasiadis, S H; Stratakis, E

    2015-01-01

    This letter reports on the femtosecond laser fabrication of gradient-wettability micro/nano- patterns on Si surfaces. The dynamics of directional droplet spreading on the surface tension gradients developed is systematically investigated and discussed. It is shown that microdroplets on the patterned surfaces spread at a maximum speed of 505 mm/sec, that is the highest velocity demonstrated so far for liquid spreading on a surface tension gradient in ambient conditions. The application of the proposed laser patterning technique for the precise fabrication of surface tension gradients for open microfluidic systems, liquid management in fuel cells and drug delivery is envisaged.

  3. Adaptive Thermostats for Noisy Gradient Systems

    CERN Document Server

    Leimkuhler, Benedict

    2015-01-01

    We study numerical methods for sampling probability measures in high dimensions where the underlying model is only approximately identified with a gradient system. Extended stochastic dynamical methods are discussed which have application to multiscale models, nonequilibrium molecular dynamics and Bayesian sampling techniques arising in emerging machine learning applications. In addition to providing a more comprehensive discussion of the foundations of these methods, we propose a new numerical method for the Adaptive Langevin/stochastic gradient Nos\\'e-Hoover thermostat that achieves a dramatic improvement in numerical efficiency over the most popular stochastic gradient methods reported in the literature. We also demonstrate that the newly-established method inherits a superconvergence property (fourth order convergence to the invariant measure for configurational quantities) recently demonstrated in the setting of Langevin dynamics. Our findings are verified by numerical experiments.

  4. Inversion gradients for acoustic VTI wavefield tomography

    KAUST Repository

    Li, Vladimir

    2017-03-21

    Wavefield tomography can handle complex subsurface geology better than ray-based techniques and, ultimately, provide a higher resolution. Here, we implement forward and adjoint wavefield extrapolation for VTI (transversely isotropic with a vertical symmetry axis) media using a generalized pseudospectral operator based on a separable approximation for the P-wave dispersion relation. This operator is employed to derive the gradients of the differential semblance optimization (DSO) and modified image-power objective functions. We also obtain the gradient expressions for a data-domain objective function that can more easily incorporate borehole information necessary for stable VTI velocity analysis. These gradients are similar to the ones obtained with a space-time finite-difference (FD) scheme for a system of coupled wave equations but the pseudospectral method is not hampered by the imprint of the shear-wave artifact. Numerical examples also show the potential advantages of the modified image-power objective function in estimating the anellipticity parameter η.

  5. The Differential Virial Theorem with Gradient Formulas for the Operators

    CERN Document Server

    Finley, James P

    2016-01-01

    A gradient dependent formula is derived for the spinless one-particle density-matrix operator z from the differential virial theorem. A gradient dependent formula is also derived for a spinless one-particle density-matrix operator that can replace the two operators of the differential virial theorem that arise from the kinetic energy operator. Other operators are also derived that can replace the operators mentioned above in the differential virial theorem; these operators depend on the real part of spinless one-particle density-matrix.

  6. Reinforcement Learning by Value Gradients

    CERN Document Server

    Fairbank, Michael

    2008-01-01

    The concept of the value-gradient is introduced and developed in the context of reinforcement learning. It is shown that by learning the value-gradients exploration or stochastic behaviour is no longer needed to find locally optimal trajectories. This is the main motivation for using value-gradients, and it is argued that learning value-gradients is the actual objective of any value-function learning algorithm for control problems. It is also argued that learning value-gradients is significantly more efficient than learning just the values, and this argument is supported in experiments by efficiency gains of several orders of magnitude, in several problem domains. Once value-gradients are introduced into learning, several analyses become possible. For example, a surprising equivalence between a value-gradient learning algorithm and a policy-gradient learning algorithm is proven, and this provides a robust convergence proof for control problems using a value function with a general function approximator.

  7. Gradient chromatofocusing high-performance liquid chromatography. II. Theoretical aspects.

    Science.gov (United States)

    Liu, Y; Anderson, D J

    1997-02-21

    This article is Part II of a series describing a newly-developed gradient chromatofocusing high-performance liquid chromatography (HPLC) technique. Theoretical aspects of the technique are discussed. In gradient chromatofocusing, the column pH gradient with respect to column distance can be varied without necessarily affecting the outlet pH gradient with respect to time. Factors influencing the value of the slope of the column pH gradient are identified through derived equations and a computer simulation model. A newly-identified parameter is introduced, column travel time, which can be uniquely varied in gradient chromatofocusing. Experiments show increased conversion of fibrinogen to denatured forms with increased column travel time. Another unique aspect of gradient chromatofocusing is that the mobile phase buffer concentration can be manipulated without necessarily affecting the outlet pH gradient slope, giving the technique expanded versatility for optimizing the separation. In the present work, the pIapparent for fibrinogen is found to increase with increased mobile phase buffer concentration.

  8. Determination of complete velocity gradient tensor by using cinematographic stereoscopic PIV in a turbulent jet

    Energy Technology Data Exchange (ETDEWEB)

    Ganapathisubramani, B. [The University of Texas at Austin, Center for Aeromechanics Research, Austin, TX (United States); Imperial College London, Department of Aeronautics, London (United Kingdom); Lakshminarasimhan, K. [The University of Texas at Austin, Center for Aeromechanics Research, Austin, TX (United States); Sandia National Laboratories, Combustion Research Facility, Livermore, CA (United States); Clemens, N.T. [The University of Texas at Austin, Center for Aeromechanics Research, Austin, TX (United States)

    2007-06-15

    Cinematographic stereoscopic PIV measurements were performed in the far field of an axisymmetric co-flowing turbulent round jet (Re{sub T}{approx}150, where Re{sub T} is the Reynolds number based on Taylor micro scale) to resolve small and intermediate scales of turbulence. The time-resolved three-component PIV measurements were performed in a plane normal to the axis of the jet and the data were converted to quasi-instantaneous three-dimensional (volumetric) data by using Taylor's hypothesis. The availability of the quasi-three-dimensional data enabled the computation of all nine components of the velocity gradient tensor over a volume. The use of Taylor's hypothesis was validated by performing a separate set of time-resolved two component ''side-view'' PIV measurements in a plane along the jet axis. Probability density distributions of the velocity gradients computed using Taylor's hypothesis show good agreement with those computed directly with the spatially resolved data. The overall spatial structure of the gradients computed directly exhibits excellent similarity with that computed using Taylor's hypothesis. The accuracy of the velocity gradients computed from the pseudo-volume was assessed by computing the divergence error in the flow field. The root mean square (rms) of the divergence error relative to the magnitude of the velocity gradient tensor was found to be 0.25, which is consistent with results based on other gradient measurement techniques. The velocity gradients, vorticity components and mean dissipation in the self-similar far field of the jet were found to satisfy the axisymmetric isotropy conditions. The divergence error present in the data is attributed to the intrinsic uncertainty associated with performing stereoscopic PIV measurements and not to the use of Taylor's hypothesis. The divergence error in the data is found to affect areas of low gradient values and manifests as nonphysical values for

  9. Gedanken Densities and Exact Constraints in Density Functional Theory

    CERN Document Server

    Perdew, John P; Sun, Jianwei; Burke, Kieron

    2014-01-01

    Approximations to the exact density functional for the exchange-correlation energy of a many-electron ground state can be constructed by satisfying constraints that are universal, i.e., valid for all electron densities. Gedanken densities are designed for the purpose of this construction, but need not be realistic. The uniform electron gas is an old gedanken density. Here, we propose a spherical two-electron gedanken density in which the dimensionless density gradient can be an arbitrary positive constant wherever the density is non-zero. The Lieb-Oxford lower bound on the exchange energy can be satisfied within a generalized gradient approximation (GGA) by bounding its enhancement factor or simplest GGA exchange-energy density. This enhancement-factor bound is well known to be sufficient, but our gedanken density shows that it is also necessary. The conventional exact exchange-energy density satisfies no such local bound, but energy densities are not unique, and the simplest GGA exchange-energy density is no...

  10. Differential heating: A versatile method for thermal conductivity measurements in high-energy-density matter

    Energy Technology Data Exchange (ETDEWEB)

    Ping, Y.; Fernandez-Panella, A.; Correa, A.; Shepherd, R.; Landen, O.; London, R. A.; Sterne, P. A.; Whitley, H. D.; Fratanduono, D.; Collins, G. W. [Lawrence Livermore National Laboratory, Livermore, California 94550 (United States); Sio, H. [Plasma Science and Fusion Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States); Boehly, T. R. [Laboratory for Laser Energetics, University of Rochester, Rochester, New York 14623 (United States)

    2015-09-15

    We propose a method for thermal conductivity measurements of high energy density matter based on differential heating. A temperature gradient is created either by surface heating of one material or at an interface between two materials by different energy deposition. The subsequent heat conduction across the temperature gradient is observed by various time-resolved probing techniques. Conceptual designs of such measurements using laser heating, proton heating, and x-ray heating are presented. The sensitivity of the measurements to thermal conductivity is confirmed by simulations.

  11. Excited state geometry optimization with the density matrix renormalization group as applied to polyenes

    CERN Document Server

    Hu, Weifeng

    2015-01-01

    We describe and extend the formalism of state-specific analytic density matrix renormalization group (DMRG) energy gradients, first used by Liu et al (J. Chem. Theor.Comput. 9, 4462 (2013)). We introduce a DMRG wavefunction maximum overlap following technique to facilitate state-specific DMRG excited state optimization. Using DMRG configuration interaction (DMRG-CI) gradients we relax the low-lying singlet states of a series of trans-polyenes up to C20H22. Using the relaxed excited state geometries as well as correlation functions, we elucidate the exciton, soliton, and bimagnon ("single-fission") character of the excited states, and find evidence for a planar conical intersection.

  12. Composition Gradient Hard Coatings by Arc Ion Plating

    Institute of Scientific and Technical Information of China (English)

    CHEN Jun; LIN Guo-qiang; WANG Fu-gang

    2004-01-01

    Arc Ion Plating can be used to synthesize multi-component composition gradient hard coatings by adjusting arc currents of metal targets. The present work aims at a comprehensive description of such a technique. The examples of TiAl multi-layer alloy coatings and (Ti, M) N composition-gradient films were taken (M representing Zr, Nb etc.) for the purpose of explaining the working process and evaluating practical effects. The results show that this technique has the advantages of easy manipulation, rapid deposition, and wide composition range.Key Words: Arc Ion plating, hard coating, composition gradient coatings

  13. Strain gradient crystal plasticity: A continuum mechanics approach to modeling micro-structural evolution

    DEFF Research Database (Denmark)

    El-Naaman, Salim Abdallah; Nielsen, Kim Lau; Niordson, Christian Frithiof

    2015-01-01

    In agreement with dislocation theory, recent experiments show, both quantitatively and qualitatively, how geometrically necessary dislocations (GNDs) distribute in dislocation wall and cell structures. Hence, GND density fields are highly localized with large gradients and discontinuities occurring...... between the cells. This behavior is not typical for strain gradient crystal plasticity models. The present study employs a higher order extension of conventional crystal plasticity theory in which the viscous slip rate is influenced by the gradients of GND densities through a back stress...

  14. Strain gradient crystal plasticity: A continuum mechanics approach to modeling micro-structural evolution

    DEFF Research Database (Denmark)

    El-Naaman, Salim Abdallah; Nielsen, Kim Lau; Niordson, Christian Frithiof

    2015-01-01

    In agreement with dislocation theory, recent experiments show, both quantitatively and qualitatively, how geometrically necessary dislocations (GNDs) distribute in dislocation wall and cell structures. Hence, GND density fields are highly localized with large gradients and discontinuities occurring...... between the cells. This behavior is not typical for strain gradient crystal plasticity models. The present study employs a higher order extension of conventional crystal plasticity theory in which the viscous slip rate is influenced by the gradients of GND densities through a back stress...

  15. First estimation of Eurasian lynx (Lynx lynx abundance and density using digital cameras and capture–recapture techniques in a German national park

    Directory of Open Access Journals (Sweden)

    Weingarth, K.

    2012-01-01

    Full Text Available Eurasian lynx are individually identifiable by their unique coat markings, making them ideal candidates for capture–recapture (CMR surveys. We evaluated the use of digital photography to estimate Eurasian lynx population abundance and density within the Bavarian Forest National Park. From November 2008 to January 2009 we placed 24 camera trap sites, each with two cameras facing each other on well–used walking tracks. The units were placed based on a systematic grid of 2.7 km. We captured five independent and three juvenile lynx and calculated abundance estimates using Program Mark. We also compared density estimates based on the MMDM method (Mean Maximum Distance Moved from telemetry data (½MMDMGPS and from camera trapping data (½MMDMCAM. We estimated that in an effectively sampled area of 664 km2 the Eurasian lynx density was 0.9 individuals/100 km2 with ½MMDMCAM. The Eurasian lynx density calculated with ½MMDMGPS was 0.4 individuals/100 km2 in an effectively sampled area of 1,381 km2. Our results suggest that long–term photographic CMR sampling on a large scale may be a useful tool to monitor population trends of Eurasian lynx in accordance with the Fauna–Flora–Habitat Directive of the European Union.

  16. Determination of the density of the defect states in Hf0.5Zr0.5O2 high-k film Deposited by using rf-magnetron sputtering technique

    Directory of Open Access Journals (Sweden)

    W. Lu

    2014-08-01

    Full Text Available A memory structure Pt/Al2O3/Hf0.5Zr0.5O2/Al2O3/p-Si was fabricated by using atomic layer deposition and rf-magnetron sputtering techniques, and its microstructure has been investigated by using the high resolution transmission electron microscopy (HRTEM. By measuring the applied gate voltage dependence of the capacitance for the memory structure, the planar density of the trapped charges in Hf0.5Zr0.5O2 high-k film was estimated as 6.63 × 1012 cm−2, indicating a body defect density of larger than 2.21 × 1019 cm−3. It is observed that the post-annealing in N2 can reduces the defect density in Hf0.5Zr0.5O2 film, which was ascribed to the occupancy of oxygen vacancies by nitrogen atoms.

  17. Predicting for Sustainable Insurance with Adaptive Gradient Methods

    Directory of Open Access Journals (Sweden)

    Parveen Sehgal

    2015-07-01

    Full Text Available This paper extends the comparison of gradient based training methods used in the construction of prediction models based upon neural network, for sustainable insurance. Here adaptive gradient based techniques are compared with simple first order gradient based technique and with some second order training techniques for learning of the networ