WorldWideScience

Sample records for delft leiden bsdl

  1. Standhouden in Delft

    NARCIS (Netherlands)

    De Jong, F.

    1997-01-01

    Technology is still masculine, and so is the Technical University at Delft. Frequently the question is asked: who were the first woman teacher and woman student? More interesting, however, is the question how women participated in this domain throughout history and how they managed to survive. This

  2. Looking back: the Delft method

    NARCIS (Netherlands)

    Makkink, H.

    2007-01-01

    One of the more unexpected innovations to come out of Delft is an unconventional method for teaching a second language. Developed 25 years ago by the applied linguistics section, the Delft method has proved highly effective, provided, that is, the students do their homework. You learn a language by

  3. The Delft-Java Engine

    NARCIS (Netherlands)

    Glossner III, C.J.

    2001-01-01

    In this dissertation, we describe the DELFT-JAVA engine - a 32-bit RISC-based architecture that provides high performance JAVA program execution. More specifically we describe a microarchitecture that accelerates JAVA execution and provide details of the DELFT-JAVA architecture for executing JAVA

  4. Learning about Factor V Leiden Thrombophilia

    Science.gov (United States)

    ... on this page Learning About Factor V Leiden Thrombophilia What is factor V Leiden thrombophilia? What are ... V Leiden Thrombophilia What is factor V Leiden thrombophilia? Factor V Leiden thrombophilia is an inherited disorder ...

  5. In Delft: a personal account.

    Science.gov (United States)

    Scheffers, W Alexander

    2015-08-01

    The author looks back on his development in microbiology and yeast research, and on the establishment in Delft of the FEMS Central Office, FEMS Publications Office and the birth of FEMS Yeast Research. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  6. Study of Delft aerospace alumni

    NARCIS (Netherlands)

    Smits, G.N.

    2008-01-01

    This thesis reports on an alumni study of the Faculty Aerospace Engineering at Delft University of Technology to discover what the impact is of the degree in aerospace engineering on an alumnus' professional success and comment on what are important qualities for aerospace engineers to have in order

  7. Factor V Leiden thrombophilia.

    Science.gov (United States)

    Kujovich, Jody Lynn

    2011-01-01

    Factor V Leiden is a genetic disorder characterized by a poor anticoagulant response to activated Protein C and an increased risk for venous thromboembolism. Deep venous thrombosis and pulmonary embolism are the most common manifestations, but thrombosis in unusual locations also occurs. The current evidence suggests that the mutation has at most a modest effect on recurrence risk after initial treatment of a first venous thromboembolism. Factor V Leiden is also associated with a 2- to 3-fold increased relative risk for pregnancy loss and possibly other obstetric complications, although the probability of a successful pregnancy outcome is high. The clinical expression of Factor V Leiden is influenced by the number of Factor V Leiden alleles, coexisting genetic and acquired thrombophilic disorders, and circumstantial risk factors. Diagnosis requires the activated Protein C resistance assay (a coagulation screening test) or DNA analysis of the F5 gene, which encodes the Factor V protein. The first acute thrombosis is treated according to standard guidelines. Decisions regarding the optimal duration of anticoagulation are based on an individualized assessment of the risks for venous thromboembolism recurrence and anticoagulant-related bleeding. In the absence of a history of thrombosis, long-term anticoagulation is not routinely recommended for asymptomatic Factor V Leiden heterozygotes, although prophylactic anticoagulation may be considered in high-risk clinical settings. In the absence of evidence that early diagnosis reduces morbidity or mortality, decisions regarding testing at-risk family members should be made on an individual basis.

  8. Genetics Home Reference: factor V Leiden thrombophilia

    Science.gov (United States)

    ... Conditions factor V Leiden thrombophilia factor V Leiden thrombophilia Enable Javascript to view the expand/collapse boxes. ... Open All Close All Description Factor V Leiden thrombophilia is an inherited disorder of blood clotting . Factor ...

  9. Delft3D turbine turbulence module

    Energy Technology Data Exchange (ETDEWEB)

    2016-04-18

    The DOE has funded Sandia National Labs (SNL) to develop an open-source modeling tool to guide the design and layout of marine hydrokinetic (MHK) arrays to maximize power production while minimizing environmental effects. This modeling framework simulates flows through and around a MHK arrays while quantifying environmental responses. As an augmented version of the Dutch company, Deltares’s, environmental hydrodynamics code, Delft3D, SNL-Delft3D includes a new module that simulates energy conversion (momentum withdrawal) by MHK devices with commensurate changes in the turbulent kinetic energy and its dissipation rate.

  10. Delft: Nieuwe natuursteen in een oude stad

    NARCIS (Netherlands)

    Nijland, T.G.; Hees, R.P.J. van; Quist, W.

    2012-01-01

    Delft is alom bekend van de Oude en de Nieuwe Kerk, beiden (deels) opgetrokken door bouwmeesters/ steenhouwers van het roemruchte Brabantse geslacht Keldermans en deels in de onvermijdelijk met deze bouwmeesters verbonden witte zandige kalksteen, meestal Lede of Balegemse steen genoemd. Met name aan

  11. Parking Spoorzone Delft: Addressing expected parking challenges 2015-2017

    OpenAIRE

    Piccot, C.; Groenendijk, L.; Rot, M.; Van der Meijs, P.; Rakers, T.; Negenborn, R.R.; Annema, J.A.; Van Pel, A; Vleugel, J.

    2014-01-01

    This project is carried out on request of the BVOW, the interest group of the neighbourhoods Olofsbuurt and Westerkwartier in Delft, in order to propose solutions for the parking issue of Spoorzone Delft expected between 2015 and 2017. They are worried that parking disturbances will emerge in their neighbourhoods when the parking places of Spoorzone Delft will be removed. Indeed, in 2015 the parking places that are currently situated below the viaduct will disappear due to the removal of the ...

  12. Forze hydrogen racing team Delft; TU Delft students develop hydrogen race-car

    NARCIS (Netherlands)

    Hartvelt, M.

    2014-01-01

    ‘Working towards a sustainable, yet exciting future’ is one of the big challenges in engineering nowadays. A group of students from the TU Delft accepted this challenge and designed a zero-emission hydrogen powered race-car. Combining green technology with racing, Forze wants to show the potential

  13. Industriel produktudvikler fra Delft University of Technology

    DEFF Research Database (Denmark)

    Hansen, Claus Thorp

    1999-01-01

    Hverdagen for konstruktører og produktudviklere i dansk industri er præget af tidspres og stærkt voksende kompleksitet, havd angår nye teknologier, mange hensyn, dokumentationstvang, kvalitetsproblemer og mange nye computerværktøjer. I dette bidrag fokuseres på hvordan morgendagens produktudvikle...... uddannes. Uddannelsen til industriel produktudvikler ved Delft University of Technology sammenlignes med IKSs uddannelse af konstruktører og produktudviklere. I sammenligningen ses på de to institutioners værdiopfattelser, uddannelsernes opbygning og kandidaternes professionalisme....

  14. Parking Spoorzone Delft: Addressing expected parking challenges 2015-2017

    NARCIS (Netherlands)

    Piccot, C.; Groenendijk, L.; Rot, M.; Van der Meijs, P.; Rakers, T.; Negenborn, R.R.; Annema, J.A.; Pel, A.; Vleugel, J.

    2014-01-01

    This project is carried out on request of the BVOW, the interest group of the neighbourhoods Olofsbuurt and Westerkwartier in Delft, in order to propose solutions for the parking issue of Spoorzone Delft expected between 2015 and 2017. They are worried that parking disturbances will emerge in their

  15. Parking Spoorzone Delft: Addressing expected parking challenges 2015-2017

    NARCIS (Netherlands)

    Piccot, C.; Groenendijk, L.; Rot, M.; Van der Meijs, P.; Rakers, T.; Negenborn, R.R.; Annema, J.A.; Pel, A.; Vleugel, J.

    2014-01-01

    This project is carried out on request of the BVOW, the interest group of the neighbourhoods Olofsbuurt and Westerkwartier in Delft, in order to propose solutions for the parking issue of Spoorzone Delft expected between 2015 and 2017. They are worried that parking disturbances will emerge in their

  16. Forze hydrogen racing team Delft; TU Delft students develop hydrogen race-car

    OpenAIRE

    Hartvelt, M.

    2014-01-01

    ‘Working towards a sustainable, yet exciting future’ is one of the big challenges in engineering nowadays. A group of students from the TU Delft accepted this challenge and designed a zero-emission hydrogen powered race-car. Combining green technology with racing, Forze wants to show the potential of hydrogen as an alternative fuel. Find out how Forze has been developing hydrogen powered racecars since 2007.

  17. Energy conservation at Brouwer Rotatie in Delft, Netherlands; Energiebesparing bij Brouwer Rotatie Delft

    Energy Technology Data Exchange (ETDEWEB)

    Jansen, S.F.H. [Verwey R.T.B., Utrecht (Netherlands)

    2000-04-01

    At Brouwer Rotatie in Delft, Netherlands, the use of waste heat from the production process to produce energy for cooling using an absorption refrigerator turned out to be a financially attractive option. By using a heat and power installation, the purchase of electricity is significantly reduced, with the thermal energy released being largely applied elsewhere at the complex. With respect to future energy policy, it is recommended to purchase energy for the Brouwer Group on a national level.

  18. Delft Mass Transport model DMT-2

    Science.gov (United States)

    Ditmar, Pavel; Hashemi Farahani, Hassan; Inacio, Pedro; Klees, Roland; Zhao, Qile; Guo, Jing; Liu, Xianglin; Sun, Yu; Riva, Ricardo; Ran, Jiangjun

    2013-04-01

    Gravity Recovery And Climate Experiment (GRACE) satellite mission has enormously extended our knowledge of the Earth's system by allowing natural mass transport of various origin to be quantified. This concerns, in particular, the depletion and replenishment of continental water stocks; shrinking of polar ice sheets; deformation of the Earth's crust triggered by large earthquakes, and isostatic adjustment processes. A number of research centers compute models of temporal gravity field variations and mass transport, using GRACE data as input. One of such models - Delft Mass Transport model - is being produced at the Delft University of Technology in collaboration with the GNSS Research Center of Wuhan University. A new release of this model, DMT-2, has been produced on the basis of a new (second) release of GRACE level-1b data. This model consists of a time-series of monthly solutions spanning a time interval of more than 8 years, starting from Feb. 2003. Each solution consists of spherical harmonic coefficients up to degree 120. Both unconstrained and optimally filtered solutions are obtained. The most essential improvements of the DMT-2 model, as compared to its predecessors (DMT-1 and DMT-1b), are as follows: (i) improved estimation and elimination of low-frequency noise in GRACE data, so that strong mass transport signals are not damped; (ii) computation of accurate stochastic models of data noise for each month individually with a subsequent application of frequency-dependent data weighting, which allows statistically optimal solutions to be compiled even if data noise is colored and gradually changes in time; (iii) optimized estimation of accelerometer calibration parameters; (iv) incorporation of degree 1 coefficients estimated with independent techniques; (v) usage of state-of-the-art background models to de-alias GRACE data from rapid mass transport signals (this includes the EOT11a model of ocean tides and the latest release of the AOD1B product describing

  19. Factor V Leiden and Inflammation

    Directory of Open Access Journals (Sweden)

    Silvia Perez-Pujol

    2012-01-01

    Full Text Available Factor V Leiden, is a variant of human factor V (FV, also known as proaccelerin, which leads to a hypercoagulable state. Along these years, factor V Leiden (FVL has been studied from the pathophysiologic point of view, and research has been focused on finding clinical approaches for the management of the FVL associated to a trombophilic state. Less attention has been paid about the possible role of FVL in inflammatory conditions known to be present in different disorders such as uremia, cirrhosis, liver transplantation, depression as well as sepsis, infection or, inflammatory bowel disease (IBD. Whether platelet FVL will increase the activation of coagulation and/or in which proportion is able to determine the final outcome in the previously mentioned inflammatory conditions is a subject that remains uncertain. This paper will review the association of FVL with inflammation. Specifically, it will analyze the important role of the endothelium and the contribution of other inflammatory components involved at both the immune and vascular levels. This paper will also try to emphasize the importance of being a FVL carrier in associations to diseases where a chronic inflammation occurs, and how this condition may be determinant in the progression and outcome of a specific clinic situation.

  20. College via iTunes U: TU Delft deelt graag kennis

    NARCIS (Netherlands)

    Suijker, P.

    2010-01-01

    De TU Delft publiceert lezingen, colleges en informatieve films op iTunes U van Apple. De auteur beschrijft waarom de TU Delft gekozen heeft voor iTunes U en op welke manier het project tot stand is gekomen.

  1. McStas-model of the delft SESANS

    DEFF Research Database (Denmark)

    Knudsen, Erik; Udby, L.; Willendrup, Peter Kjær

    2011-01-01

    present the first virtual data from it, using a refracting prism-like sample model. In consequence, polarisation instrumentation is now included natively in the McStas kernel, including options for magnetic fields and a number of utility components. This development has brought us to a point where......We present simulation results taking first virtual data from a model of the Spin-Echo Small Angle Scattering (SESANS) instrument situated in Delft, in the framework of the McStas Monte Carlo software package. The main focus has been on making a model of the Delft SESANS instrument, and we can now...

  2. McStas-model of the delft SESANS

    Science.gov (United States)

    Knudsen, E.; Udby, L.; Willendrup, P. K.; Lefmann, K.; Bouwman, W. G.

    2011-06-01

    We present simulation results taking first virtual data from a model of the Spin-Echo Small Angle Scattering (SESANS) instrument situated in Delft, in the framework of the McStas Monte Carlo software package. The main focus has been on making a model of the Delft SESANS instrument, and we can now present the first virtual data from it, using a refracting prism-like sample model. In consequence, polarisation instrumentation is now included natively in the McStas kernel, including options for magnetic fields and a number of utility components. This development has brought us to a point where realistic models of polarisation-enabled instrumentation can be built.

  3. The Delft innovation method: A design thinker's guide to innovation

    NARCIS (Netherlands)

    Buijs, J.A.

    2012-01-01

    This paper will introduce a new book on innovating, the process that leads to innovation(s). It is based on the experiences in the innovation and creativity field from the Delft University of Technology as well as from independent consultancy. These experiences are condensed in an overall method for

  4. Archives, Collections and Curatorship. The Delft Chair Collection

    NARCIS (Netherlands)

    Van Wijk, C.

    2011-01-01

    Last year, in May, the building of the Faculty of Architecture at the Delft Technical University was destroyed almost entirely by fire. Fortunately, some parts of the building escaped the flames and, after a few days, the library collection of over 40,000 titles was saved, as was the chair collectio

  5. Neue Miolispa aus dem Leidener Museum

    NARCIS (Netherlands)

    Kleine, R.

    1917-01-01

    Gelegentlich einer umfassenden Aufarbeitung der Gattung Miolispa Pascoe hat mir das Leidener Museum in dankenswerter Weise das noch unbestimmte Material zur Verfügung gestellt, unter welchem sich zwei neue Arten vorfanden. Beide stammen aus Neu-Guinea. Trotz des herrschenden Weltkrieges und der

  6. Mechanisms of the Factor V Leiden Paradox

    NARCIS (Netherlands)

    Stralen, van K.J.; Doggen, C.J.M.; Bezemer, I.D.; Pomp, E.R.; Lisman, T.; Rosendaal, F.R.

    2008-01-01

    Objective— Carriers of the factor V Leiden mutation (FVL-carriers) have a substantially increased risk of deep venous thrombosis (DVT), whereas the risk of pulmonary embolism (PE) is only mildly increased compared with noncarriers. So far few studies have investigated possible mechanisms for this so

  7. Mechanisms of the factor V Leiden paradox

    NARCIS (Netherlands)

    van Stralen, K. J.; Doggen, C. J. M.; Bezemer, I. D.; Pomp, E. R.; Lisman, T.; Rosendaal, F. R.

    2008-01-01

    Objective-Carriers of the factor V Leiden mutation (FVL-carriers) have a substantially increased risk of deep venous thrombosis (DVT), whereas the risk of pulmonary embolism (PE) is only mildly increased compared with noncarriers. So far few studies have investigated possible mechanisms for this so-

  8. Neue Miolispa aus dem Leidener Museum

    NARCIS (Netherlands)

    Kleine, R.

    1917-01-01

    Gelegentlich einer umfassenden Aufarbeitung der Gattung Miolispa Pascoe hat mir das Leidener Museum in dankenswerter Weise das noch unbestimmte Material zur Verfügung gestellt, unter welchem sich zwei neue Arten vorfanden. Beide stammen aus Neu-Guinea. Trotz des herrschenden Weltkrieges und der Schw

  9. Validation of Delft3D as a Coastal Surge and Inundation Prediction System

    Science.gov (United States)

    2016-04-07

    modeling system Delft3D that includes surge and inundation prediction. We have developed a GUI based on the Delft DashBoard tool that helps process ...predictions. 1.2 Delft3d modeling suite The Delft3D suite of models is a fully integrated software suite for 2-dimensional (2D) and 3- dimensional (3D... Engineering , 73, 58-70. Booij, N., R. Ris and L. Holthuijsen, 1999. A third-generation wave model for coastal regions, Part I, Model description and

  10. Factor V Leiden: a case study and review.

    Science.gov (United States)

    Slusher, Kimberlee B

    2010-01-01

    Venous thromboembolism accounts for approximately 600,000 hospitalizations and 50,000 deaths per year in the United States. Many inherited blood disorders predispose patients to this disorder. The most common of these disorders is factor V Leiden. Factor V Leiden thrombophilia is an inherited disorder, mutation, of the mechanisms of blood clotting. The mutation causes resistance to activated protein C and thus causes a defect in the natural anticoagulation system. This predisposes patients to recurrent deep vein thrombosis and in combination with other risk factors can cause significant morbidity. This article discusses the pathophysiology, disease characteristics, risk factors for venous thromboembolism, diagnosis and testing, management of factor V Leiden, and implications for nursing in regard to factor V Leiden. The frequency of factor V Leiden and its prevalence in thromboembolic disease emphasize the need for nursing professionals from many diverse backgrounds to better understand the consequences of the factor V Leiden mutation.

  11. Factor V Leiden and Cardiopulmonary Bypass.

    Science.gov (United States)

    Uppal, Victor; Rosin, Mark; Marcoux, Jo-Anne; Olson, Marnie; Bezaire, Jennifer; Dalshaug, Gregory

    2015-12-01

    We present a case of a patient with factor V Leiden with an antithrombin III activity of 67% who received a successful aortic valve replacement supported by cardiopulmonary bypass (CPB). A safe level of anticoagulation was achieved by monitoring activated clotting time (ACT) and heparin concentration ensuring adequate anticoagulation throughout the procedure. Results from ACT, heparin dose response, heparin protamine titration, and thrombelastography are given. Factor V Leiden patients can be safely anti-coagulated using heparin for CPB procedures when monitored with ACT, heparin protamine titration, and thrombelastography. Postoperative chest tube losses were 360 mL, less than half our institutional average. Anticoagulation for the pre-and post-operative phase is also discussed.

  12. Fuel shuffling optimization for the Delft research reactor

    Energy Technology Data Exchange (ETDEWEB)

    Geemert, R. van; Hoogenboom, J.E.; Gibcus, H.P.M. [Delft Univ. of Technology, Interfaculty Reactor Inst., Delft (Netherlands); Quist, A.J. [Delft Univ., Fac. of Applied Mathematics and Informatics, Delft (Netherlands)

    1997-07-01

    A fuel shuffling optimization procedure is proposed for the Hoger Onderwijs Reactor (HOR) in Delft, the Netherlands, a 2 MWth swimming-pool type research reactor. In order to cope with the fluctuatory behaviour of objective functions in loading pattern optimization, the proposed cyclic permutation optimization procedure features a gradual transition from global to local search behaviour via the introduction of stochastic tests for the number of fuel assemblies involved in a cyclic permutation. The possible objectives and the safety and operation constraints, as well as the optimization procedure, are discussed, followed by some optimization results for the HOR. (author)

  13. Evaluation of the Sustainable Development Graduation Track at Delft University of Technology

    Science.gov (United States)

    De Werk, G.; Kamp, L. M.

    2008-01-01

    This paper evaluates the sustainable development graduation track at TU Delft. This track can be followed by all students of TU Delft. It consists of an interdisciplinary colloquium "Technology in Sustainable Development", 300 h of self-chosen courses on sustainable development and a graduation project in which sustainable development is…

  14. CWI and TU Delft at TREC 2013: Contextual Suggestion, Federated Web Search, KBA, and Web Tracks

    NARCIS (Netherlands)

    Bellogín Kouki, A.; Gebremeskel, G.G.; He, J.; Lin, J.J.P.; Said, A.; Samar, T.; Vries, A.P. de; Vuurens, J.B.P.

    2014-01-01

    This paper provides an overview of the work done at the Centrum Wiskunde & Informatica (CWI) and Delft University of Technology (TU Delft) for different tracks of TREC 2013. We participated in the Contextual Suggestion Track, the Federated Web Search Track, the Knowledge Base Acceleration (KBA) Trac

  15. Fire and collapse, Faculty of Architecture building, Delft University of Technology: Data collection and preliminary analyses

    NARCIS (Netherlands)

    Meacham, B.; Park, H.; Engelhardt, M.; Kirk, A.; Kodur, V.; Straalen, IJ.J.; Maljaars, J.; Weeren, K. van; Feijter, R. de; Both, K.

    2010-01-01

    On the morning of May 13, 2008, a fire that started in a coffee vending machine on the 6th floor of the 13-story Faculty of Architecture Building at the Delft University of Technology (TUD), Delft, the Netherlands, quickly developed into an extreme loading event. Although all building occupants evac

  16. Evaluation of the Sustainable Development Graduation Track at Delft University of Technology

    Science.gov (United States)

    De Werk, G.; Kamp, L. M.

    2008-01-01

    This paper evaluates the sustainable development graduation track at TU Delft. This track can be followed by all students of TU Delft. It consists of an interdisciplinary colloquium "Technology in Sustainable Development", 300 h of self-chosen courses on sustainable development and a graduation project in which sustainable development is…

  17. Mogelijke oorzaken van van verschillen in houdbaarheid in snijhyacint Delft Blue

    NARCIS (Netherlands)

    Vreeburg, P.J.M.; Korsuize, C.A.

    2007-01-01

    De cultivar ‘Delft Blue’ is de belangrijkste snijhyacint. De presentatie van Delft Blue in knopstadium is erg goed door veelal dikke trossen met veel nagels, die goed blauw kleuren. De ervaring is echter dat deze cultivar op de vaas tegen kan vallen doordat de steel de zware bloemtros niet kan drage

  18. Fire and collapse, Faculty of Architecture building, Delft University of Technology: Data collection and preliminary analyses

    NARCIS (Netherlands)

    Meacham, B.; Park, H.; Engelhardt, M.; Kirk, A.; Kodur, V.; Straalen, IJ.J.; Maljaars, J.; Weeren, K. van; Feijter, R. de; Both, K.

    2010-01-01

    On the morning of May 13, 2008, a fire that started in a coffee vending machine on the 6th floor of the 13-story Faculty of Architecture Building at the Delft University of Technology (TUD), Delft, the Netherlands, quickly developed into an extreme loading event. Although all building occupants

  19. [Activated protein C resistance and factor V Leiden: clinical interest].

    Science.gov (United States)

    Guermazi, S; Znazen, R

    2011-10-01

    Activated protein C resistance (APCR) is a coagulation abnormality often linked to FV Leiden mutation, a single nucleotide G1691A substitution resulting in arginine 506→glutamine missense factor V mutation. FV Leiden has a frequency of 20 to 30% in groups of patients with venous thrombosis while it is of 4 to 10% in normal subjects. FV Leiden is considered as a weak risk factor of thrombosis except in homozygote. FV Leiden is implicated in deep venous thrombosis occurrence. Duration of oral anticoagulant treatment is six months in patients developing a first venous thrombosis except in patients with combined defects or a clinical context suggesting a high risk of severe relapse. Detection of APCR by coagulation methods is often used in first intention with a high specificity if plasmas tested are diluted in factor V deficient plasma. Genotyping study is essential to establish the heterozygote or homozygote statute and certain teams perform it directly. Nevertheless, APCR not related to FV Leiden could be an independent thrombosis risk factor. APCR and FV Leiden are included in laboratory investigations of thrombophilic markers in patients less than 50 years with venous thrombosis. In arterial thrombosis, FV Leiden implication is weak or absent. FV Leiden increases the risk of thrombosis in other situations as in patients with cancer. An association with recurrent miscarriages and other vasculoplacental complications is also reported in many studies but the data concerning the efficacy of antithrombotic treatment to prevent recurrence are currently insufficient. Copyright © 2009 Elsevier Masson SAS. All rights reserved.

  20. The Dermaptera in the Museums at Leiden and Amsterdam

    NARCIS (Netherlands)

    Boeseman, M.

    1954-01-01

    The collections of Dermaptera in the Rijksmuseum van Natuurlijke Historie at Leiden and the Zoölogisch Museum at Amsterdam, referred to in the present paper as "Museum Leiden" and "Museum Amsterdam", consist of numerous specimens including many types described by previous authors and a fairly large

  1. Genetic modulation of the FV(Leiden)/normal FV ratio and risk of venous thrombosis in factor V Leiden heterozygotes.

    Science.gov (United States)

    Segers, O; Simioni, P; Tormene, D; Bulato, C; Gavasso, S; Rosing, J; Castoldi, Elisabetta

    2012-01-01

    The factor (F)V Leiden mutation causes activated protein C (APC) resistance by decreasing the susceptibility of FVa to APC-mediated inactivation and by impairing the APC-cofactor activity of FV in FVIIIa inactivation. However, APC resistance and the risk of venous thromboembolism (VTE) vary widely among FV Leiden heterozygotes. Common F5 genetic variation probably contributes to this variability. APC resistance was determined in 250 FV Leiden heterozygotes and 133 normal relatives using the prothrombinase-based assay, which specifically measures the susceptibility of plasma FVa to APC. The effects of 12 F5 single-nucleotide polymorphisms (SNPs) on the normalized APC sensitivity ratio (nAPCsr) and on FV levels were determined by multiple regression analysis. In FV Leiden heterozygotes,VTE risk increased with increasing nAPCsr, reaching an odds ratio (OR) of 9.9 (95% confidence interval [CI] 1.2–80.5) in the highest nAPCsr quartile. The minor alleles of several F5 SNPs, including 327 A/G (Q51Q), 409 G/C (D79H), 2663 A/G(K830R, T2 haplotype), 6533 T/C (M2120T) and 6755 A/G (D2194G, R2 haplotype), increased the nAPCsr in FV Leiden heterozygotes, but not in their normal relatives. Most of these effects could be attributed to a shift in the FV(Leiden)/normal FV ratio. Four FV Leiden heterozygotes with extremely high nAPCsr turned out to be pseudo-homozygotes, i.e. they carried a deleterious mutation on the non-Leiden allele. In FV Leiden heterozygotes, the prothrombinase-based nAPCsr is a marker of VTE risk and is modulated by common F5 SNPs that affect the FV(Leiden)/normal FV ratio in plasma.

  2. 'Most rare workmen': optical practitioners in early seventeenth-century Delft.

    Science.gov (United States)

    Zuidervaart, Huib J; Rijks, Marlise

    2015-03-01

    A special interest in optics among various seventeenth-century painters living in the Dutch city of Delft has intrigued historians, including art historians, for a long time. Equally, the impressive career of the Delft microscopist Antoni van Leeuwenhoek has been studied by many historians of science. However, it has never been investigated who, at that time, had access to the mathematical and optical knowledge necessary for the impressive achievements of these Delft practitioners. We have tried to gain insight into Delft as a 'node' of optical knowledge by following the careers of three minor local figures in early seventeenth-century Delft. We argue that through their work, products, discussions in the vernacular and exchange of skills, rather than via learned publications, these practitioners constituted a foundation on which the later scientific and artistic achievements of other Delft citizens were built. Our Delft case demonstrates that these practitioners were not simple and isolated craftsmen; rather they were crucial components in a network of scholars, savants, painters and rich virtuosi. Decades before Vermeer made his masterworks, or Van Leeuwenhoek started his famous microscopic investigations, the intellectual atmosphere and artisanal knowledge in this city centred on optical topics.

  3. Leiden Mutation and the Course of Severe Acute Pancreatitis

    Directory of Open Access Journals (Sweden)

    A. V. Ershov

    2013-01-01

    Full Text Available Objective: to evaluate the impact of Leiden mutation on the course of severe acute pancreatitis. Subjects and methods. One hundred and twelve people were examined. Group 1 comprised 50 patients diagnosed with severe acute pancreatitis without coagulation factor V (Leiden mutation. Group 2 included 42 patients with severe acute pancreatitis who were found to have Leiden mutation. Acute pancreatitis was first diagnosed in both groups. Group 3 consisted of 20 apparently healthy individuals (a control group. The severity of the underlying disease was determined in accordance with the clinical and laboratory parameters recommended by the I. I. Dzhanelidze Saint Petersburg Research Institute of Emergence Care. Results. This investigation revealed an association of Leiden mutation with trends in the development of acute pancreatitis. Group 2 exhibited a more severe disease: large focal pancreatic necrosis was twice more common and infectious complications developed more frequently; more aggressive and radical treatments were more often used. The patients with Leiden mutation had higher mortality rates (33% in the Leiden mutation group and 24% in the non-mutation group. Conclusion. The findings should be kept in mind in elaborating new diagnostic methods and principles in the treatment of the underlying disease and in the prevention of its complications in patients with severe acute pancreatitis. Key words: acute pancreatitis, Leiden mutation.

  4. Livedoid vasculopathy and its association with factor V Leiden mutation.

    Science.gov (United States)

    Yong, Angeline Anning; Tan, Audrey Wei Hsia; Giam, Yoke Chin; Tang, Mark Boon Yang

    2012-12-01

    Livedoid vasculopathy is a rare chronic relapsing disorder characterised by recurrent painful thrombotic and vasculitic ulcers on the legs. We present the cases of two Indian women with livedoid vasculopathy that were found to be associated with an underlying factor V Leiden heterozygous mutation. There were no other thrombotic manifestations, and livedoid vasculopathy was the sole presenting feature of the factor V Leiden mutation, although this could also be coincidental. Initial treatment with high-dose immunosuppressive therapy was suboptimal, and the addition of pentoxifylline and antiplatelet therapy was crucial in achieving disease control and remission. These cases highlight the possible association with an underlying prothrombotic disorder, such as factor V Leiden mutation, in patients with livedoid vasculopathy. Although this association is relatively uncommon, it is more relevant to Indian patients, as the presence of factor V Leiden mutation is highest in this ethnicity as compared to the local Malay and Chinese populations.

  5. Improved formula. Delft University of Technology wants to win back the World Cup with solar car Nuna6; Verbeterde formule. TU Delft wil met Nuna6 wereldbeker heroveren

    Energy Technology Data Exchange (ETDEWEB)

    Hoving, D.

    2011-10-14

    Students of the Technical University of Delft participate in the international biennial competition for solar cars in Australia for the sixth time. Again, they managed to produce and design a lighter, smaller and more aerodynamic solar car than the previous one. [Dutch] Studenten van de Technische Universiteit Delft doen voor de zesde keer mee aan de internationale tweejaarlijkse wedstrijd voor zonnewagens in Australie. Ze zijn er weer in geslaagd een auto te ontwerpen en te fabriceren die lichter, kleiner en aerodynamischer is dan de vorige.

  6. A New View of Delft: Delft geodesists and industrial designers show 3D perspective based on seventeenth century drawings and prints

    NARCIS (Netherlands)

    De Boo, M.

    2001-01-01

    In order to gain a comprehensive view of the consequences of large infrastructure operations, good imaging techniques are essential to present projects in a clear manner. At the Delft Department of Geodetic Engineering, Virtual Reality techniques have been linked for the first time to geographical i

  7. Updated Delft Mass Transport model DMT-2: computation and validation

    Science.gov (United States)

    Hashemi Farahani, Hassan; Ditmar, Pavel; Inacio, Pedro; Klees, Roland; Guo, Jing; Guo, Xiang; Liu, Xianglin; Zhao, Qile; Didova, Olga; Ran, Jiangjun; Sun, Yu; Tangdamrongsub, Natthachet; Gunter, Brian; Riva, Ricardo; Steele-Dunne, Susan

    2014-05-01

    A number of research centers compute models of mass transport in the Earth's system using primarily K-Band Ranging (KBR) data from the Gravity Recovery And Climate Experiment (GRACE) satellite mission. These models typically consist of a time series of monthly solutions, each of which is defined in terms of a set of spherical harmonic coefficients up to degree 60-120. One of such models, the Delft Mass Transport, release 2 (DMT-2), is computed at the Delft University of Technology (The Netherlands) in collaboration with Wuhan University. An updated variant of this model has been produced recently. A unique feature of the computational scheme designed to compute DMT-2 is the preparation of an accurate stochastic description of data noise in the frequency domain using an Auto-Regressive Moving-Average (ARMA) model, which is derived for each particular month. The benefits of such an approach are a proper frequency-dependent data weighting in the data inversion and an accurate variance-covariance matrix of noise in the estimated spherical harmonic coefficients. Furthermore, the data prior to the inversion are subject to an advanced high-pass filtering, which makes use of a spatially-dependent weighting scheme, so that noise is primarily estimated on the basis of data collected over areas with minor mass transport signals (e.g., oceans). On the one hand, this procedure efficiently suppresses noise, which are caused by inaccuracies in satellite orbits and, on the other hand, preserves mass transport signals in the data. Finally, the unconstrained monthly solutions are filtered using a Wiener filter, which is based on estimates of the signal and noise variance-covariance matrices. In combination with a proper data weighting, this noticeably improves the spatial resolution of the monthly gravity models and the associated mass transport models.. For instance, the computed solutions allow long-term negative trends to be clearly seen in sufficiently small regions notorious

  8. Diagnosis and management of factor V Leiden.

    Science.gov (United States)

    Campello, Elena; Spiezia, Luca; Simioni, Paolo

    2016-12-01

    The discovery of the factor V Leiden (FVL) missense mutation (Arg506Gln) causing factor V resistance to the anticoagulant action of activated protein C was a landmark that allowed a better understanding of the basis of inherited thrombotic risk. FVL mutation is currently the most common known hereditary defect predisposing to venous thrombosis. Areas covered: Novel data-driven FVL diagnosis and therapeutic approaches in the management of FVL carriers in various clinical settings. Brief conclusions on topics of direct clinical relevance including currently available indications for primary and secondary prophylaxis, the management of female, pediatric carriers and asymptomatic relatives. Latest evidence on the association between FVL and cancer, as well as the possible use of direct oral anticoagulant therapy. Expert commentary: Although FVL diagnosis nowadays is highly accurate, many doubts remain regarding the best management and therapeutic protocols. The main role of clinicians is to tailor therapeutic strategies to carriers and their relatives. High familial penetrance, distinctive aspects of the first thrombotic event (provoked/unprovoked, age, etc.) and laboratory biomarkers can guide the optimal management of secondary antithrombotic prophylaxis, primary prophylaxis in asymptomatic individuals, and whether to screen relatives.

  9. Influence of the factor V Leiden mutation on infectious disease susceptibility and outcome

    DEFF Research Database (Denmark)

    Benfield, Thomas L; Dahl, Mortens; Nordestgaard, Borge G

    2005-01-01

    The effect of the coagulation factor V Leiden mutation on infectious disease susceptibility and outcome is controversial.......The effect of the coagulation factor V Leiden mutation on infectious disease susceptibility and outcome is controversial....

  10. 21 CFR 864.7280 - Factor V Leiden DNA mutation detection systems.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Factor V Leiden DNA mutation detection systems....7280 Factor V Leiden DNA mutation detection systems. (a) Identification. Factor V Leiden deoxyribonucleic acid (DNA) mutation detection systems are devices that consist of different reagents...

  11. Factor v Leiden homozygous genotype and pregnancy outcomes.

    Science.gov (United States)

    Biron-Andréani, Christine; Bauters, Anne; Le Cam-Duchez, Véronique; Delahousse, Bénédicte; Lequerrec, Agnès; Dutrillaux, Fabienne; Boinot, Catherine; Saladin-Thiron, Catherine; Polack, Benoit; Gruel, Yves; Morange, Pierre-Emmanuel

    2009-12-01

    To assess the rate of early (first trimester) and late (second and third trimester) fetal loss in women who are factor V Leiden homozygous. Between December 1995 and February 2007, consecutive, unrelated white women who were factor V Leiden homozygous and who had been pregnant at least once were recruited from 10 French hemostasis units. For reasons of comparison, we included women who were factor V Leiden heterozygous and a group of noncarriers. The frequency of early and late fetal loss was assessed retrospectively and compared among the three groups. The effect of concomitant thrombophilic abnormalities was evaluated. The overall pregnancy outcome was reported. We analyzed 240 thromboprophylaxis-free pregnancies in 95 women who were factor V Leiden homozygous, 425 in 195 women who were factor V Leiden heterozygous, and 182 in 73 women who were noncarriers. The risk of late fetal loss was higher in women who were homozygous (13/95, 13.7%) compared with those who were noncarriers (1/73, 1.4%, odds ratio 11.41, 95% confidence interval 1.46-89.46, P=.002), whereas it was similar in women who were heterozygous and in noncarriers (6/195, 3.1% compared with 1/73, 1.4%, P=.68). The percentage of women with early fetal loss was similar in the three groups (P=.81). The live-birth rate was 80%, 84%, and 85%, respectively, for women who where homozygous, heterozygous, and noncarriers (P=.88). The factor V Leiden homozygous genotype increases the risk of late fetal loss. However, the overall likelihood of a positive outcome is high in our series of women who were homozygous. III.

  12. Anticoagulation duration in heterozygous factor V Leiden: a decision analysis.

    Science.gov (United States)

    Donovan, Anna K; Smith, Kenneth J; Ragni, Margaret V

    2013-01-01

    Current anticoagulation guidelines suggest that optimal anticoagulation duration for unprovoked venous thromboembolism is determined by an individual risk assessment, balancing risks of anticoagulation bleeding with venous thromboembolism recurrence. Among individuals heterozygous for the factor V Leiden mutation, while venous thromboembolism recurrence risk is greater, the risk for bleeding is recognized to be lower, suggesting longer duration anticoagulation could be considered. The objective of this study was to compare standard vs. lifelong anticoagulation in 20-year-old factor V Leiden heterozygotes with unprovoked venous thromboembolism. A Markov state-transition model was used, incorporating risks of major, minor, and fatal anticoagulation bleeding, bleeding and thromboembolism morbidity and mortality, and quality of life utilities. Model parameter values favoring lifelong anticoagulation in factor V Leiden heterozygotes were determined in sensitivity analyses. Outcomes were in quality-adjusted life years, discounted at 3% per year. In general population groups with odds ratios for venous thromboembolism recurrence and anticoagulation bleeding of 1.0, a short-term anticoagulation strategy gained 0.09 quality-adjusted life years more than a lifelong anticoagulation strategy. By contrast, in factor V Leiden heterozygotes, lifetime anticoagulation was favored if their relative risk of venous thromboembolism was greater than 1.07 or their relative risk for bleeding was less than 0.91. Results were relatively insensitive to individual variation in other parameter values. Lifelong anticoagulation may benefit individuals heterozygous for factor V Leiden and previous idiopathic venous thromboembolism. Studies assessing bleeding risk with anticoagulation in factor V Leiden heterozygotes and the costs of indefinite anticoagulation are needed to determine if lifelong anticoagulation is the optimal strategy. © 2013 Elsevier Ltd. All rights reserved.

  13. [Idiopathic intracranial hypertension and factor V Leiden mutation].

    Science.gov (United States)

    Younes, S; Aissi, M; Chérif, Y; Daoussi, N; Boughammoura, A; Frih Ayed, M; Sfar, M H; Jerbi, S

    2014-07-01

    Activated proteinC resistance is a frequent prothrombotic abnormality. In most cases it is due to factorV Leiden mutation by nucleotide G1691A substitution. This recently described thrombophilic defect of activated proteinC resistance has been postulated to be implicated in the pathogenesis of idiopathic intracranial hypertension (IIH). We report a case of factorV Leiden mutation in association with IIH and their likely link and implication in the management of IIH. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  14. Neue Grabwespen aus der Sammlung des Leidener Museums

    NARCIS (Netherlands)

    Handlirsch, Anton

    1895-01-01

    Durch die Güte der Herren Director Dr. F. A. Jentink und Conservator C. Ritsema erhielt ich anlässlich einer monographischen Arbeit das reiche Nyssoniden- und Bembecidenmaterial des Leidener Museums zur Revision zugeschikt. Unter den vielen interessanten Arten dieser Sammlung befinden sich auch 5 fü

  15. MicroDOT: Design of a 10W prototype of the Delft offshore turbine

    NARCIS (Netherlands)

    Kempenaar, A.S.; Diepeveen, N.F.B.; Jarquin Laguna, A.

    2011-01-01

    The Delft Offshore Turbine (DOT) project is a research project within DUWIND. The main objective of the project is to reduce the costs of offshore wind energy through technical solutions. A defining characteristic of the DOT concept is the fluid power transmission. The next step for the DOT concept

  16. Eco-runner team Delft; the most fuel efficient vehicle in the world

    NARCIS (Netherlands)

    Rijks, F.

    2014-01-01

    The slogan, ‘Eco-Runner: the most fuel efficient vehicle in the world’ of the Eco-Runner Team Delft says it all: designing and building the most fuel efficient vehicle in the world. The Eco-Runner Team is a ‘D:DREAM Team’ where students from various faculties work together to design and build extrem

  17. Making the user really matter – a test case from Delft

    NARCIS (Netherlands)

    Mantel, E.; Van Wezenbeek, W.J.S.M.

    2014-01-01

    How do you become even more successful if you are already successful? That is a question that TU Delft Library asks itself regularly. In the last few years, it has seen that its transformation into a library learning centre was a good move, but there is more. Even more focus can be on the client, th

  18. Eco-runner team Delft; the most fuel efficient vehicle in the world

    NARCIS (Netherlands)

    Rijks, F.

    2014-01-01

    The slogan, ‘Eco-Runner: the most fuel efficient vehicle in the world’ of the Eco-Runner Team Delft says it all: designing and building the most fuel efficient vehicle in the world. The Eco-Runner Team is a ‘D:DREAM Team’ where students from various faculties work together to design and build

  19. Essential Skills for System Dynamics Practitioners: A Delft University of Technology Perspective

    NARCIS (Netherlands)

    Meyers, W.K.; Slinger, J.H.; Pruyt, E.; Yucel, G.; Van Daalen, C.

    2010-01-01

    There is little consensus on the skills set required of a system dynamics practitioner. In this paper we use the teaching approach and learning goals of the system dynamics courses at the Delft University of Technology as a starting point to explore the development of system dynamics modeling

  20. Creating the integral engineer: Combining development education, sustainability, entrepreneurship and technology at Delft University of Technology

    NARCIS (Netherlands)

    Zwarteveen, J.W.; Blom, E.M.; Vastbinder, B.; Brezet, J.C.

    2010-01-01

    A modern engineer is more than a technical specialist. Training an integral engineer requires education in non-technical skills, including social and ethical aspects. Therefore, Delft University of Technology (DUT) introduced sustainable development and entrepreneurship into its bachelor and master

  1. Observations from the fire and collapse of the Faculty of Architecture Building, Delft University of Technology

    NARCIS (Netherlands)

    Engelhardt, M.D.; Meacham, B.; Kodur, V.; Kirk, A.; Park, H.; Straalen, IJ.J. van; Maljaars, J.; Weeren, K. van; Feijter, R. de; Both, K.

    2013-01-01

    On May 13, 2008, a fire occurred at the Faculty of Architecture Building at the Delft University of Technology (TUD) in the Netherlands. The fire ultimately led to the collapse of a major portion of the building. Data was collected on this fire event by an international team that included

  2. The trail of six design projects in the Delft bachelor Aerospace Engineering

    NARCIS (Netherlands)

    Kamp, A.

    2012-01-01

    Tomorrow’s engineers are required to have a good balance between deep working knowledge of engineering sciences and engineering skills. In the Bachelor Aerospace Engineering at TU Delft, students are educated to master these competences so that they are ready to engineer when they graduate. The

  3. One Thousand Flowers in Delft: A Bottom-up approach to improve teaching international students

    NARCIS (Netherlands)

    Brummelink, M.L.; Frantzeskaki, N.; Klaassen, R.G.; Blom, E.M.; Kooij, R.E.; Kroesen, J.O.; Stadler D.I.; Boogaard, M.E.D. van den

    2009-01-01

    Delft University of Technology (DUT) is becoming more and more an international community. Since lectures on master level are all conducted in English, the numbers of students from foreign countries attending DUT are rising and international cooperation within educational projects is a necessity. To

  4. Delft Dashboard: a quick setup tool for coastal and estuarine models

    Science.gov (United States)

    Nederhoff, C., III; Van Dongeren, A.; Van Ormondt, M.; Veeramony, J.

    2016-02-01

    We developed easy-to-use Delft DashBoard (DDB) software for the rapid set-up of coastal and estuarine hydrodynamic and basic morphological numerical models. In the "Model Maker" toolbox, users have the capability to set-up Delft3D models, in a minimal amount of time (in the order of a hour), for any location in the world. DDB draws upon public internet data sources of bathymetry and tidesto construct the model. With additional toolboxes, these models can be forced with parameterized hurricane wind fields, uplift of the sea surface due to tsunamis nested in publically available ocean models and forced with meteo data (wind speed, pressure, temperature) In this presentation we will show the skill of a model which is setup with Delft Dashboard and compare it to well-calibrated benchmark models. These latter models have been set-up using detailed input data and boundary conditions. We have tested the functionality of Delft DashBoard and evaluate the performance and robustness of the DDB model system on a variety of cases, ranging from a coastal to basin models. Furthermore, we have performed a sensitivity study to investigate the most critical physical and numerical processes. The software can benefit operational modellers, as well as scientists and consultants.

  5. Creating the integral engineer: Combining development education, sustainability, entrepreneurship and technology at Delft University of Technology

    NARCIS (Netherlands)

    Zwarteveen, J.W.; Blom, E.M.; Vastbinder, B.; Brezet, J.C.

    2010-01-01

    A modern engineer is more than a technical specialist. Training an integral engineer requires education in non-technical skills, including social and ethical aspects. Therefore, Delft University of Technology (DUT) introduced sustainable development and entrepreneurship into its bachelor and master

  6. DelftCluster Railway transition zones & Switches: Factual report long-term measurements

    NARCIS (Netherlands)

    Hartman, A.D.; Hölscher, P.

    2009-01-01

    For the Delft Cluster project “Railway Transition zones” extensive field-testing has been performed. Testing took place on the railway track Gouda-Goverwelle (GoGo) on a culvert and on a Switch. Many different parties were involved in the testing and numerous different types of tests were performed.

  7. DelftCluster Railway transition zones and switches: Factual report fieldtest monitoring

    NARCIS (Netherlands)

    Hartman, A.D.

    2009-01-01

    For the Delft Cluster project ‘Railway transition zones and switches’, extensive field-testing has been carried out. At a location east of the railway station Gouda Goverwelle (GoGo) the behavior of a track and soil at a culvert and a switch are studied. This report is part of a series of reports de

  8. Delft Cluster Railway transition zones & Switches: Factual report short-term measurement 2009

    NARCIS (Netherlands)

    Hölscher, P.; Hartman, A.D.

    2009-01-01

    For the Delft Cluster 'Railway transition zones and switches' project, extensive measurements are made. The measurements are divided into three types. The short-term measurement of may 2009 is the subject of this report. The long-term and soil investigation measurements are described in other report

  9. Test facility development for testing of micro-thrusters at TU-Delft

    NARCIS (Netherlands)

    Zandbergen, B.T.C.; Janssens, S.; Valente, F.; Perez-Grande, D.; Koopmans, R.

    2010-01-01

    The development of highly integrated and compact micro-satellites and the need for a higher precision of positioning and attitude control in space missions has stimulated the development of micro-propulsion systems. At TU-Delft, investigations are focusing on non-chemical, thermal propulsion. TU-Del

  10. The trail of six design projects in the Delft bachelor Aerospace Engineering

    NARCIS (Netherlands)

    Kamp, A.

    2012-01-01

    Tomorrow’s engineers are required to have a good balance between deep working knowledge of engineering sciences and engineering skills. In the Bachelor Aerospace Engineering at TU Delft, students are educated to master these competences so that they are ready to engineer when they graduate. The bach

  11. Evaluating Research in Context: Pilot Study at Faculty of Architecture TU Delft

    NARCIS (Netherlands)

    Van der Meulen, B.; Daemen, F.; Van Drooge, L.; De Jong, S.; Spaapen, J.; Wamelink, F.; Van den Besselaar, P.

    2010-01-01

    The vice chancellor of Delft University of Technology, Prof.dr.ir. J. Fokkema, introduced a pilot Evaluating Research in Context (ERiC) at the Faculty of Architecture. The Faculty of Architecture perceives a serious confl ict between the demands and criteria in evaluation procedures and the ambition

  12. Essential Skills for System Dynamics Practitioners: A Delft University of Technology Perspective

    NARCIS (Netherlands)

    Meyers, W.K.; Slinger, J.H.; Pruyt, E.; Yucel, G.; Van Daalen, C.

    2010-01-01

    There is little consensus on the skills set required of a system dynamics practitioner. In this paper we use the teaching approach and learning goals of the system dynamics courses at the Delft University of Technology as a starting point to explore the development of system dynamics modeling skills

  13. Proceedings of the 1st workshop on aspect reverse-engineering, Delft, 09.11.2004

    NARCIS (Netherlands)

    Tourwé, T.; Bruntink, M.; Marin, A.M.; Shepherd, D.

    2005-01-01

    This technical report contains the papers submitted to and presented at the 1st Workshop on Aspect Reverse-Engineering, held in conjunction with the 11th Working Conference on Reverse Engineering (WCRE), in Delft, The Netherlands. The aims of this workshop was to bring together researchers and pract

  14. Why is factor V Leiden so rare in the Basques?

    Science.gov (United States)

    Bauduer, F

    2015-05-01

    Factor V Leiden is the most common inherited trait in Caucasians that predisposes individuals to venous thrombosis. However, it is almost absent amongst the Basque people that live in the south western part of Europe. To explain this finding, we speculate upon the putative contribution of various evolutionary forces through which the Basque genome may have been shaped. © 2015 International Society on Thrombosis and Haemostasis.

  15. Note sur les Buprestides du Museum de Leiden

    NARCIS (Netherlands)

    Thery, A.

    1935-01-01

    M. le Dr. H. C. Blöte, entomologiste du Rijksmuseum van Natuurlijke Historie à Leiden (Hollande) m'a confié l'étude de matériaux indéterminés appartenant aux collections de cet établissement, parmi ceux-ci j'ai trouvé quelques espèces nouvelles dont je donne ci-dessous la description. Les types de t

  16. Factor V Leiden and Prothrombin Mutations in South of Iran

    Directory of Open Access Journals (Sweden)

    M Karimi

    2007-01-01

    Full Text Available ABSTRACT: Introduction & Objective: Factor V Leiden and prothrombin mutation are not common but they are involved in pediatric thrombosis. The aim of this study was to evaluate the frequency of factor V Leiden & prohtrombin mutation in healthy population of Shiraz, south of Iran. Materials & Methods: In this cross-sectional study 195 healthy people (97 female and 98 male were randomly selected. Peripheral white blood cells obtained from 5 ml blood contained 1-2 mg/ml K2- EDTA. Genomic DNA extraction was performed following the protocol described by Miller et al. PCR amplification was carried out in 25μl reaction volume containing 0.5 units Taq polymerase, 200μM dNTP, 500 μM of each of the previously described primers. After initial denaturation, 35 cycles at 95◦c for 30s, and 72◦c for 20s and followed extention by 72 for 10 min were performed. About 10μl of PDR product was digested with MNI I or Mbo restriction enzymes. Results: In this study we determined factor V Leiden in 8 (4.1% and prothrombin mutation in 6 individual (3.07% of 198 cases in heterozygous form. No homozygous was seen for any of the mutations. Only one case presented a double heterozygous for factor V and prothrombin in this cohort. Conclusion: Several studies of factor V leiden and prothrombin mutations in the East of Asia showed the higher frequency of these mutations in Iran.

  17. Survival advantage of heterozygous fV Leiden carriers in murine sepsis

    Science.gov (United States)

    Kerschen, Edward; Hernandez, Irene; Zogg, Mark; Maas, Matthias; Weiler, Hartmut

    2015-01-01

    Summary Background The high allelic frequency of the prothrombotic Leiden polymorphism in human blood coagulation factor V (fV) has been speculated to reflect positive selection during evolution. Heterozygous Leiden carriers enrolled in the placebo arm of the PROWESS sepsis trial, and heterozygous Leiden mice challenged with endotoxin both showed reduced mortality, whereas homozygous Leiden mice were not protected from lethal endotoxemia. Follow-up analyses of clinical outcomes, and of mouse models of infection with various pathogens remained inconclusive. Objective To establish whether aPC-resistance of fV Leiden modifies the outcome of bacterial infection in murine sepsis models. Methods Homozygous and heterozygous fV Leiden mice were subjected to gram-positive (S.aureus) or gram-negative (Y.pestis; E.coli) septic peritonitis, or polymicrobial, focal septic peritonitis induced by cecal ligation and puncture (CLP); and the effect of fV Leiden on 7-day survival and bacterial dissemination was assessed. Outcomes were compared to the sepsis survival of mice with genetically impaired hemostasis (hemophilia A, thrombocytopenia, thrombin receptor PAR4 deficiency, protein C receptor ProcR/EPCR-deficiency). Results Heterozygous, but not homozygous Leiden mice were protected from lethal infection with highly virulent S.aureus and Y.pestis strains. FV Leiden did not affect the outcome of sepsis induced by CLP, staphylokinase-deficient S.aureus, Pla-deficient Y.pestis, or E.coli. Thrombocytopenia, deficiency of PAR1 or PAR4 did not affect S.aureus sepsis survival, whereas hemophilia A increased mortality. ProcR-deficiency selectively abolished the survival advantage of heterozygous Leiden mice. Conclusions In mice, heterozygous fV Leiden carriers are protected from sepsis mortality after infection with clinically relevant human bacterial pathogens. PMID:25690763

  18. Survival advantage of heterozygous factor V Leiden carriers in murine sepsis.

    Science.gov (United States)

    Kerschen, E; Hernandez, I; Zogg, M; Maas, M; Weiler, H

    2015-06-01

    The high allelic frequency of the prothrombotic Leiden polymorphism in human blood coagulation factor V (FV) has been speculated to reflect positive selection during evolution. Heterozygous Leiden carriers enrolled in the placebo arm of the PROWESS sepsis trial and heterozygous Leiden mice challenged with endotoxin both showed reduced mortality, whereas homozygous Leiden mice were not protected from lethal endotoxemia. Follow-up analyses of clinical outcomes and of mouse models of infection with various pathogens remained inconclusive. To establish whether activated protein C resistance of FV Leiden modifies the outcome of bacterial infection in murine sepsis models. Homozygous and heterozygous FV Leiden mice were subjected to gram-positive (S. aureus) or gram-negative (Y. pestis; E. coli) septic peritonitis or polymicrobial, focal septic peritonitis induced by cecal ligation and puncture. The effect of FV Leiden on 7-day survival and bacterial dissemination was assessed. Outcomes were compared with the sepsis survival of mice with genetically impaired hemostasis (hemophilia A, thrombocytopenia, thrombin receptor PAR4 [protease activated receptor 4] deficiency, endothelial protein C receptor [ProcR/EPCR] deficiency). Heterozygous, but not homozygous, Leiden mice were protected from lethal infection with highly virulent S. aureus and Y. pestis strains. FV Leiden did not affect the outcome of sepsis induced by cecal ligation and puncture, staphylokinase-deficient S. aureus, Pla-deficient Y. pestis, or E. coli. Thrombocytopenia, deficiency of PAR1 or PAR4 did not affect S. aureus sepsis survival, whereas hemophilia A increased mortality. ProcR deficiency selectively abolished the survival advantage of heterozygous Leiden mice. In mice, heterozygous FV Leiden carriers are protected from sepsis mortality after infection with clinically relevant human bacterial pathogens. © 2015 International Society on Thrombosis and Haemostasis.

  19. Delft3D-FLOW on PRACE infrastructures for real life hydrodynamic applications.

    Science.gov (United States)

    Donners, John; Genseberger, Menno; Jagers, Bert; de Goede, Erik; Mourits, Adri

    2013-04-01

    PRACE, the Partnership for Advanced Computing in Europe, offers access to the largest high-performance computing systems in Europe. PRACE invites and helps industry to increase their innovative potential through the use of the PRACE infrastructure. This poster describes different efforts to assist Deltares with porting the open-source simulation software Delft3D-FLOW to PRACE infrastructures. Analysis of the performance on these infrastructures has been done for real life flow applications. Delft3D-FLOW is a 2D and 3D shallow water solver which calculates non-steady flow and transport phenomena resulting from tidal and meteorological forcing on a curvilinear, boundary fitted grid in Cartesian or spherical coordinates. It also includes a module which sediment transport (both suspended and bed total load) and morphological changes for an arbitrary number of cohesive and non-cohesive fractions. As Delft3D-FLOW has been developed over several decades, with a variety of functionality and over 350k lines of source code, porting to PRACE infrastructures needs some effort. At the moment Delft3D-FLOW uses MPI with domain decomposition in one direction as its parallellisation approach. Because it is hard to identify scaling issues if one immediately starts with a complex case with many features enabled, different cases with increasing complexity have been used to investigate scaling of this parallellisation approach on several PRACE platforms. As a base reference case we started with a schematic high-resolution 2D hydrodynamic model of the river Waal that turned out to be surprisingly well-suited to the highly-parallel PRACE machines. Although Delft3D-FLOW employs a sophisticated build system, several modifications were required to port it to most PRACE systems due to the use of specific, highly-tuned compilers and MPI-libraries. After this we moved to a 3D hydrodynamic model of Rotterdam harbour that includes sections of the rivers Rhine and Meuse and a part of the North

  20. Homozygous factor V Leiden and double heterozygosity for factor V Leiden and prothrombin mutation.

    Science.gov (United States)

    Saemundsson, Ymir; Sveinsdottir, Signý Vala; Svantesson, Henrik; Svensson, Peter J

    2013-10-01

    The most common forms of familial thrombophilia are factor V Leiden (FVL) and prothrombin mutation (PTM). Homozygous FVL and PTM have long been feared conditions thought to cause high rates of morbidity and mortality. To analyse clinical features in patients with homozygous FVL and PTM, as well as patients with double heterozygosity for FVL and PTM. All patients with homozygous FVL, PTM or double heterozygosity in the MATS database of 1465 consecutive unselected patients were analysed regarding age at inclusion venous thromboembolism (VTE), age at first thrombosis, recurrence, clinical course and acquired risk factors. We found 36 patients homozygous for FVL. Patients homozygous for FVL were younger than controls at group level (56 ± 18 vs. 63 ± 17, p < 0.02). Homozygous women were younger than female controls (50 ± 19 vs. 63 ± 18, p < 0.002). No difference was observed when comparing male subjects. Women were younger than men at inclusion thrombosis (50 ± 19 vs. 65 ± 14, p < 0.02) and at first thrombosis (47 ± 19 vs. 64 ± 14, p < 0.01). Deep venous thrombosis (DVT) was seen in 33 patients (92 %), 6 (17 %) had pulmonary embolism (PE) and 3 (8 %) had combined DVT and PE. PE was less frequent in homozygous FVL women compared to female controls (p < 0.03). VTE recurred in 3 subjects during the duration of the study. Odds ratio for VTE in homozygous FVL patients compared to controls was 13.9 (95 % CI 9.9-19.7). We found no subjects with homozygous PTM. Double heterozygosity for FVL and PTM was seen in 12 subjects. There was no difference in age at inclusion VTE between double heterozygotes and controls (59 ± 16 vs. 63 ± 17, ns.). DVT was seen in 92 % at inclusion, 8 % had PE. Mean age at first VTE was 52 ± 17 (27-82). Consecutive homozygous FVL patients had a higher age at first thrombosis than previously described. Homozygous females are affected at an earlier age than homozygous men and female controls. It seems that

  1. Characterization of atherosclerotic lesions in apo E3-leiden transgenic mice

    NARCIS (Netherlands)

    Leppänen, P.; Luoma, J.S.; Hofker, M.H.; Havekes, L.M.; Ylä-Herttuala, S.

    1998-01-01

    Apo E3-leiden transgenic mice express human dysfunctional apo E variant and develop hyperlipidemia and atherosclerosis on a high fat/high cholesterol diet. We characterized diet-induced atherosclerotic lesions in apo E3-leiden transgenic mice using immunocytochemical methods in order to examine foam

  2. Preferential association of apolipoprotein E Leiden with very low density lipoproteins of human plasma

    NARCIS (Netherlands)

    Fazio, S.; Horie, Y.; Weisgraber, K.H.; Havekes, L.M.; Rall Jr., S.C.

    1993-01-01

    Apolipoprotein (apo) E Leiden is a rare variant of human apoE characterized by defective receptor binding and associated with dominant transmission of type III hyperlipoproteinemia. In heterozygotes, apoE Leiden is present in higher concentrations in both total plasma and very low density

  3. Factor XIII Va134Leu and the risk of venous thromboembolism in factor V Leiden carriers

    NARCIS (Netherlands)

    Franco, RF; Middeldorp, S; Meinardi, [No Value; van Pampus, ECM; Reitsma, PH

    2000-01-01

    A mutation in factor XIII (Val34Leu) was reported to protect against Venous thromboembolism. We evaluated the effect of Val34Leu on thrombotic risk in 352 factor V Leiden carriers who were first-degree relatives of 132 thrombotic propositi carrying factor V Leiden. The total observation period was

  4. Factor V Leiden mutation: An added risk in single ventricle palliation

    OpenAIRE

    R Saileela; C Shanthi; Ravi Agarwal; Raghavan Subramanyan; K M Cherian

    2012-01-01

    We present the case report of a child with Factor V Leiden mutation who underwent Fontan procedure. Thromboembolism is a widely recognized complication of the Fontan procedure and its modifications. Factor V Leiden mutation, being a hypercoagulable state, posed a higher risk for thromboembolism in this child. Appropriate measures taken before and after surgery prevented postoperative coagulopathy.

  5. Progression and regression of atherosclerosis in APOE3-Leiden transgenic mice : An immunohistochemical study

    NARCIS (Netherlands)

    Gijbels, M.J.J.; Cammen, M. van der; Laan, L.J.W. van der; Emeis, J.J.; Havekes, L.M.; Hofker, M.H.; Kraal, G.

    1999-01-01

    Apolipoprotein E3-Leiden (APOE3-Leiden) transgenic mice develop hyperlipidemia and are highly susceptible to diet-induced atherosclerosis. We have studied the progression and regression of atherosclerosis using immunohistochemistry. Female transgenic mice were fed a moderate fat diet to study athero

  6. Factor XIII Va134Leu and the risk of venous thromboembolism in factor V Leiden carriers

    NARCIS (Netherlands)

    Franco, RF; Middeldorp, S; Meinardi, [No Value; van Pampus, ECM; Reitsma, PH

    2000-01-01

    A mutation in factor XIII (Val34Leu) was reported to protect against Venous thromboembolism. We evaluated the effect of Val34Leu on thrombotic risk in 352 factor V Leiden carriers who were first-degree relatives of 132 thrombotic propositi carrying factor V Leiden. The total observation period was 2

  7. Characterization of atherosclerotic lesions in apo E3-leiden transgenic mice

    NARCIS (Netherlands)

    Leppänen, P.; Luoma, J.S.; Hofker, M.H.; Havekes, L.M.; Ylä-Herttuala, S.

    1998-01-01

    Apo E3-leiden transgenic mice express human dysfunctional apo E variant and develop hyperlipidemia and atherosclerosis on a high fat/high cholesterol diet. We characterized diet-induced atherosclerotic lesions in apo E3-leiden transgenic mice using immunocytochemical methods in order to examine foam

  8. Weldenkende burgers en Oranjeliefhebbers. Patriotten en Prinsgezinden in Leiden, 1775-1795

    NARCIS (Netherlands)

    de Jong, E.H.

    2014-01-01

    This book deals with the conflicts between Patriots and Orangists in Leiden, which will be assessed with the help of the theory of collective action by the American sociologist Charles Tilly. The urban economy in Leiden was dominated by a textile industry that concentrated on exports and was very

  9. Factor V Leiden associated with flap loss in microsurgical breast reconstruction.

    Science.gov (United States)

    Khansa, Ibrahim; Colakoglu, Salih; Tomich, David C; Nguyen, Minh-Doan; Lee, Bernard T

    2011-07-01

    Two cases are reported of flap loss following microsurgical perforator flap breast reconstruction in patients diagnosed with a factor V Leiden mutation. Factor V Leiden is the most common inherited cause of hypercoagulability, leading to an increased risk of thrombotic events. The first patient underwent a deep inferior epigastric artery perforator flap and then had recurrent arterial thrombosis both intraoperatively and postoperatively. This patient was subsequently diagnosed with a factor V Leiden mutation. The second patient had a known factor V Leiden mutation and underwent a superior gluteal artery perforator flap, which developed thrombosis and flap loss 2 days later. Preoperative assessment of a personal or family history of unexplained venous or arterial thrombosis should prompt suspicion of a factor V Leiden mutation. This mutation places patients at high risk for thromboembolic events in microvascular breast reconstruction, particularly when oral contraceptives or tamoxifen are used in conjunction. Copyright © 2011 Wiley-Liss, Inc.

  10. Activated protein C resistance testing for factor V Leiden.

    Science.gov (United States)

    Kadauke, Stephan; Khor, Bernard; Van Cott, Elizabeth M

    2014-12-01

    Activated protein C resistance assays can detect factor V Leiden with high accuracy, depending on the method used. Factor Xa inhibitors such as rivaroxaban and direct thrombin inhibitors including dabigatran, argatroban, and bivalirudin can cause falsely normal results. Lupus anticoagulants can cause incorrect results in most current assays. Assays that include dilution into factor V-deficient plasma are needed to avoid interference from factor deficiencies or elevations, which can arise from a wide variety of conditions such as warfarin, liver dysfunction, or pregnancy. The pros and cons of the currently available assays are discussed. © 2014 Wiley Periodicals, Inc.

  11. Factor V Leiden Is Associated with Higher Risk of Deep Venous Thrombosis of Large Blood Vessels

    Science.gov (United States)

    Arsov, Todor; Miladinova, Daniela; Spiroski, Mirko

    2006-01-01

    Aim To determine the prevalence of factor V Leiden mutation in patients with different presentation of venous thromboembolic disease and healthy individuals in the Republic of Macedonia. Methods The retrospective case-control study involved 190 patients with venous thromboembolic disease and 200 healthy individuals, who were screened for the presence of factor V Leiden mutation, using a polymerase chain reaction-restriction fragment length polymorphism method. The prevalence of factor V Leiden was analyzed according to the localization of thrombosis, presence of risk factors, and family history of thrombosis. The odds of deep venous thrombosis were calculated with respect to the presence of factor V Leiden mutation. Results The prevalence of factor V Leiden mutation among patients with venous thromboembolic disease was 21.1%, compared with 5.5% in the healthy individuals. Factor V Leiden positive patients had the first episode of deep venous thrombosis at a younger age, and the prevalence of the mutation was the highest among patients with a positive family history of thrombosis (33.9%, P = 0.003) and in patients with deep venous thrombosis affecting a large blood vessel (37.7%, P = 0.001). The prevalence of factor V Leiden mutation was lower in patients with calf deep venous thrombosis and primary thromboembolism (13.3% and 13.1%, respectively; P>0.05). The odds ratio for iliofemoral or femoral deep venous thrombosis in factor V Leiden carriers was 10.4 (95% confidence interval, 4.7-23.1). Conclusion The prevalence of factor V Leiden mutation was high in patients with venous thromboembolic disease and healthy individuals in the Republic of Macedonia. Factor V Leiden carriers have the highest odds of developing deep venous thrombosis affecting a large venous blood vessel. PMID:16758522

  12. Daily hydro- and morphodynamic simulations at Duck, NC, USA using Delft3D

    Science.gov (United States)

    Penko, Allison; Veeramony, Jay; Palmsten, Margaret; Bak, Spicer; Brodie, Katherine; Hesser, Tyler

    2017-04-01

    Operational forecasting of the coastal nearshore has wide ranging societal and humanitarian benefits, specifically for the prediction of natural hazards due to extreme storm events. However, understanding the model limitations and uncertainty is as equally important as the predictions themselves. By comparing and contrasting the predictions of multiple high-resolution models in a location with near real-time collection of observations, we are able to perform a vigorous analysis of the model results in order to achieve more robust and certain predictions. In collaboration with the U.S. Army Corps of Engineers Field Research Facility (USACE FRF) as part of the Coastal Model Test Bed (CMTB) project, we have set up Delft3D at Duck, NC, USA to run in near-real time, driven by measured wave data at the boundary. The CMTB at the USACE FRF allows for the unique integration of operational wave, circulation, and morphology models with real-time observations. The FRF has an extensive array of in-situ and remotely sensed oceanographic, bathymetric, and meteorological data that is broadcast in near-real time onto a publically accessible server. Wave, current, and bed elevation instruments are permanently installed across the model domain including 2 waverider buoys in 17-m and 26-m water depths at 3.5-km and 17-km offshore, respectively, that record directional wave data every 30-min. Here, we present the workflow and output of the Delft3D hydro- and morphodynamic simulations at Duck, and show the tactical benefits and operational potential of such a system. A nested Delft3D simulation runs a parent grid that extends 12-km in the along-shore and 3.5-km in the cross-shore with 50-m resolution and a maximum depth of approximately 17-m. The bathymetry for the parent grid was obtained from a regional digital elevation model (DEM) generated by the Federal Emergency Management Agency (FEMA). The inner nested grid extends 1.8-km in the along-shore and 1-km in the cross-shore with 5-m

  13. Myotonic Dystrophy-1 Complicated by Factor-V (Leiden Mutation

    Directory of Open Access Journals (Sweden)

    Josef Finsterer

    2015-01-01

    Full Text Available Objectives. Presence of a factor-V Leiden mutation in a patient with myotonic dystrophy type 1 (DM1 has been reported only once. Here we report the second DM1 patient carrying a factor-V mutation who died from long-term complications of this mutation. Case Report. A 66-year-old DM1 patient with multi-organ-disorder syndrome developed a first deep venous thrombosis (DVT and consecutive pulmonary embolism (PE at age 50 y. Acetyl-salicylic acid was given. One year later he experienced a second DVT; that is why phenprocoumon was started. Despite anticoagulation, he experienced a third DVT bilaterally and a second PE bilaterally at 61 y; that is why a vena cava filter was additionally deployed. Despite therapeutic anticoagulation, he experienced a vena cava filter thrombosis at age 62 y. Genetic workup revealed a heterozygous factor-V mutation in addition to a CTG-repeat expansion of 500. As a consequence of PE he developed chronic obstructive pulmonary disease and experienced recurrent pulmonary infections, which were lastly responsible for decease at age 66 y despite intensive care measures. Conclusion. The heterozygous Leiden mutation may severely affect DM1 patients to such a degree that they die from its complications. If DM1 patients present with unusual manifestations, search for causes other than a CTG-repeat expansion is indicated.

  14. The absence of factor V Leiden mutation in Malays with recurrent spontaneous abortions.

    Science.gov (United States)

    Yusoff, Narazah Mohd; Abdullah, Wan Zaidah; Ghazali, Selamah; Othman, Mohd Shukri; Baba, Abdul Aziz; Abdullah, Norazmi; Isa, Mohd Nizam; Chong, Chan Li

    2002-05-01

    The objectives of this study were to investigate the prevalence of factor V Leiden mutation in Malay women with recurrent spontaneous abortion and to clarify the contribution of the factor V Leiden mutation to recurrent miscarriages in these women. A prospective case control study between June 1999 and April 2000. Hospital University Science of Malaysia, Kubang Kerian, Kelantan, and Maternal and Child Health Clinic, Pasir Mas, Kelantan, Malaysia. A total of 46 Malay women with a history of three or more first or second trimester miscarriages were studied. The control group consisted of 46 parous women without obstetric complications. Diagnosis of factor V Leiden mutation was made by examination of factor V Leiden allele product following Mnl I digestion of factor V Leiden alleles amplified by polymerase chain reaction. None of the 46 women with recurrent spontaneous abortion carried the mutation. Also, we found no subject carrying the factor V Leiden alleles in the control group. These results suggest that that there is no association between the factor V Leiden mutation and recurrent spontaneous abortion in the Malay population.

  15. HI shells in the Leiden/Argentina/Bonn HI survey

    CERN Document Server

    Ehlerova, S

    2013-01-01

    We analyse the all-sky Leiden/Argentina/Bonn HI survey, where we identify shells belonging to the Milky Way. We used an identification method based on the search of continuous regions of a low brightness temperature that are compatible with given properties of HI shells. We found 333 shells in the whole Galaxy. The size distribution of shells in the outer Galaxy is fitted by a power law with the coefficient of 2.6 corresponding to the index 1.8 in the distribution of energy sources. Their surface density decreases exponentially with a scale length of 2.8 kpc. The surface density of shells with radii >= 100 pc in the solar neighbourhood is around 4 per kpc^2 and the 2D porosity is approximately 0.7.

  16. The relationship between FV Leiden and pulmonary embolism

    Directory of Open Access Journals (Sweden)

    Hooper W Craig

    2001-11-01

    Full Text Available Abstract Pulmonary embolism (PE is one of the leading causes of in-patient hospital deaths. As a consequence, the identification of hemostatic variables that could identify those at risk would be important in reducing mortality. It has previously been thought that deep vein thrombosis and PE are a single disease entity and would, therefore, have the same risk factors. This view is changing, however, with the realization that the prevalence of FV Leiden, a recognized genetic risk factor for deep vein thrombosis, may be a 'milder' genetic risk factor for PE. These observations suggest that PE is not only associated with a different set of risk factors, but may be reflective of a different clot structure.

  17. Factor V Leiden in women: a thrombotic risk factor or an evolutionary advantage?

    Science.gov (United States)

    Franchini, Massimo; Lippi, Giuseppe

    2011-04-01

    Factor V Leiden is a common gain-of-function gene mutation resulting in a genetic predisposition to thromboembolic complications. Growing evidence in the literature indicates an interaction between factor V Leiden thrombophilia and acquired prothrombotic conditions such as contraceptive use or hormone replacement therapy, resulting in an increased risk of venous thromboembolism (VTE). Similarly, when combined with the prothrombotic influence of pregnancy, women who are carriers of factor V Leiden are faced with an increased risk of adverse pregnancy outcomes, including VTE, pre-eclampsia, fetal loss, placental abruption, and fetal growth restriction. The results of the most important meta-analyses on the relationship between inherited (factor V Leiden) and acquired thrombophilia in women are analyzed in this review, along with the possible evolutionary role of this mutation. © Thieme Medical Publishers.

  18. The Leiden Ranking 2011/2012: Data collection, indicators, and interpretation

    CERN Document Server

    Waltman, Ludo; Kosten, Joost; Noyons, Ed C M; Tijssen, Robert J W; van Eck, Nees Jan; van Leeuwen, Thed N; van Raan, Anthony F J; Visser, Martijn S; Wouters, Paul

    2012-01-01

    The Leiden Ranking 2011/2012 is a ranking of universities based on bibliometric indicators of publication output, citation impact, and scientific collaboration. The ranking includes 500 major universities from 41 different countries. This paper provides an extensive discussion of the Leiden Ranking 2011/2012. The ranking is compared with other global university rankings, in particular the Academic Ranking of World Universities (commonly known as the Shanghai Ranking) and the Times Higher Education World University Rankings. Also, a detailed description is offered of the data collection methodology of the Leiden Ranking 2011/2012 and of the indicators used in the ranking. Various innovations in the Leiden Ranking 2011/2012 are presented. These innovations include (1) an indicator based on counting a university's highly cited publications, (2) indicators based on fractional rather than full counting of collaborative publications, (3) the possibility of excluding non-English language publications, and (4) the us...

  19. Lectures on Innovation in Building Technology: Lecture Articles for Students of Architecture Delft 1992-2015 / Nottingham 2005-2011

    NARCIS (Netherlands)

    Eekhout, A.J.C.M.

    2016-01-01

    This book is a collection of articles written in recent years and used in lectures for students at the Faculty of Architecture at Delft and at Nottingham University. The lectures and articles are based on a mixture of innovations in academia and industry. They elucidate the relationship between arch

  20. Distribution of some hydrocarbons in ambient air near Delft and the influence on the formation of secondary air pollutants

    NARCIS (Netherlands)

    Bos, R.; Guicherit, R.; Hoogeveen, A.

    1977-01-01

    The relative concentrations of hydrocarbons in the atmosphere may provide information concerning their origin. It appears that the hydrocarbon composition measured in Delft (The Netherlands) is entirely different for northern and southern wind directions. This points to different sources. The most

  1. [R]MIT Research Centre at Delft University of Technology: A Bridge between Research, Education, Society and Profession

    Science.gov (United States)

    Zijlstra, Hielkje

    2009-01-01

    In 2006, we launched the [R]MIT Research Centre (Modification, Intervention Transformation) at the Faculty of Architecture at Delft University of Technology. [R]MIT was founded to respond to the need for an integrated, multi-disciplinary approach to the transformation of the built environment. [R]MIT aims to bring momentum to the renewal of…

  2. Lectures on Innovation in Building Technology: Lecture Articles for Students of Architecture Delft 1992-2015 / Nottingham 2005-2011

    NARCIS (Netherlands)

    Eekhout, A.J.C.M.

    2016-01-01

    This book is a collection of articles written in recent years and used in lectures for students at the Faculty of Architecture at Delft and at Nottingham University. The lectures and articles are based on a mixture of innovations in academia and industry. They elucidate the relationship between

  3. Set-up and validation of a Delft-FEWS based coastal hazard forecasting system

    Science.gov (United States)

    Valchev, Nikolay; Eftimova, Petya; Andreeva, Nataliya

    2017-04-01

    European coasts are increasingly threatened by hazards related to low-probability and high-impact hydro-meteorological events. Uncertainties in hazard prediction and capabilities to cope with their impact lie in both future storm pattern and increasing coastal development. Therefore, adaptation to future conditions requires a re-evaluation of coastal disaster risk reduction (DRR) strategies and introduction of a more efficient mix of prevention, mitigation and preparedness measures. The latter presumes that development of tools, which can manage the complex process of merging data and models and generate products on the current and expected hydro-and morpho-dynamic states of the coasts, such as forecasting system of flooding and erosion hazards at vulnerable coastal locations (hotspots), is of vital importance. Output of such system can be of an utmost value for coastal stakeholders and the entire coastal community. In response to these challenges, Delft-FEWS provides a state-of-the-art framework for implementation of such system with vast capabilities to trigger the early warning process. In addition, this framework is highly customizable to the specific requirements of any individual coastal hotspot. Since its release many Delft-FEWS based forecasting system related to inland flooding have been developed. However, limited number of coastal applications was implemented. In this paper, a set-up of Delft-FEWS based forecasting system for Varna Bay (Bulgaria) and a coastal hotspot, which includes a sandy beach and port infrastructure, is presented. It is implemented in the frame of RISC-KIT project (Resilience-Increasing Strategies for Coasts - toolKIT). The system output generated in hindcast mode is validated with available observations of surge levels, wave and morphodynamic parameters for a sequence of three short-duration and relatively weak storm events occurred during February 4-12, 2015. Generally, the models' performance is considered as very good and

  4. Family history of venous thromboembolism and identifying factor V Leiden carriers during pregnancy.

    Science.gov (United States)

    Horton, Amanda L; Momirova, Valerija; Dizon-Townson, Donna; Wenstrom, Katharine; Wendel, George; Samuels, Philip; Sibai, Baha; Spong, Catherine Y; Cotroneo, Margaret; Sorokin, Yoram; Miodovnik, Menachem; O'Sullivan, Mary J; Conway, Deborah; Wapner, Ronald J

    2010-03-01

    To estimate whether there is a correlation between family history of venous thromboembolism and factor V Leiden mutation carriage in gravid women without a personal history of venous thromboembolism. This is a secondary analysis of a prospective observational study of the frequency of pregnancy-related thromboembolic events among carriers of the factor V Leiden mutation. Family history of venous thromboembolism in either first- or second-degree relatives was self-reported. Sensitivity, specificity, and positive and negative predictive values of family history to predict factor V Leiden mutation carrier status were calculated. Women without a personal venous thromboembolism history and with available DNA were included (n=5,168). One hundred forty women (2.7% [95% confidence interval (CI) 2.3-3.2%]) were factor V Leiden mutation-positive. Four hundred twelve women (8.0% [95% CI 7.3-8.7%]) reported a family history of venous thromboembolism. Women with a positive family history were twofold more likely to be factor V Leiden mutation carriers than those with a negative family history (23 of 412 [5.6%] compared with 117 of 4,756 [2.5%], Pfactor V Leiden carriers were 16.4% (95% CI 10.7-23.6%), 92.3% (95% CI 91.5-93.0%), and 5.6% (95% CI 3.6-8.3%), respectively. Although a family history of venous thromboembolism is associated with factor V Leiden mutation in thrombosis-free gravid women, the sensitivity and positive predictive values are too low to recommend screening women for the factor V Leiden mutation based solely on a family history.

  5. Factor V Leiden and inflammatory bowel disease: a systematic review and meta-analysis.

    Science.gov (United States)

    Liang, Jie; Wu, Shengjun; Feng, Bin; Lei, Shaoni; Luo, Guanhong; Wang, Jingbo; Li, Kai; Li, Xiaohua; Xie, Huahong; Zhang, Dexin; Wang, Xin; Wu, Kaichun; Miao, Danmin; Fan, Daiming

    2011-10-01

    Recent studies proved that inflammatory bowel disease (IBD) patients had a higher risk of thromboembolism and a Factor V Leiden mutation that prevents the efficient inactivation of factor V, which leads to thromboembolism and thus contributes to a high potential risk of IBD. However, the relationship between Factor V Leiden mutation and IBD remains controversial. We conducted a systematic review with meta-analysis of studies assessing the association of Factor V Leiden mutation with the risk of IBD in humans. We extracted the number of IBD and control subjects with or without Factor V Leiden mutation from each study and conducted this analysis using a fixed-effects model. Nineteen studies met the inclusion criteria and were included in the meta-analysis. No significant heterogeneity was found in results across the 19 studies (I (2) = 18.8%, P = 0.23), which showed a slight but not significant increase in the risk of IBD with Factor V Leiden mutation in the general population (summary odds ratio [OR] 1.13, 95% confidence interval [CI] 0.87-1.46). Taking into account ethnic differences, further study exhibited a slight but not significant increase in risk of IBD with Factor V Leiden mutation in Europeans (summary OR 1.20, 95% CI 0.88-1.64). However, Factor V Leiden mutation was significantly associated with a higher risk of thromboembolism in IBD patients (summary OR 5.30, 95% CI 2.25-12.48). No publication bias was found in this study. This meta-analysis indicated that although Factor V Leiden mutation was not significantly associated with the risk of IBD, it was significantly associated with a higher risk of thromboembolism in IBD patients.

  6. Fator V de Leiden na doença de Legg-Calvé-Perthes Leiden's V-factor in Legg-Calvé-Perthes disease

    Directory of Open Access Journals (Sweden)

    Lia Lira Olivier Sanders

    2009-01-01

    Full Text Available Trobofilias hereditárias têm sido implicadas na patogênese da doenca de Legg-Calvé-Perthes. Uma investigação do fator de risco hereditário mais comum para hipercoagulabilidade - a mutação no gene do fator V (fator V de Leiden - foi conduzida em 20 pacientes com Legg-Calvé-Perthes e 214 controles sadios. A prevalência do fator V de Leiden foi maior nos pacientes com Legg-Calvé-Perthes que no grupo controle (30 vs. 1,87%. A razão de chances (odds ratio para o desenvolvimento de Legg-Calvé-Perthes foi de 22,5 (pInherited tendency to hypercoagulability has been suggested as a cause of vascular thrombosis resulting in Legg-Calvé-Perthes disease. An investigation of the most common inherited risk factor for hypercoagulability - the mutation in the V-factor gene (Leiden's V-factor - was carried out among 20 Patients diagnosed with Legg- Calvé-Perthes disease. Patients were compared with 214 healthy controls. The prevalence of the Leiden's V-factor was higher in patients with Legg-Calvé-Perthes disease than in controls (30% vs. 1,87%. The odds ratio for the development of Legg-Calvé-Perthes disease in the presence of the Leiden's V-factor mutation was 22,5 (p<0,05; confidence interval: 5,68-89.07. These data suggest the Leiden's V-factor as an inherited risk factor for hypercoagulability associated with the development of Legg-Calvé-Perthes disease.

  7. Operational Satellite Based Flood Mapping Using the Delft-FEWS System

    Science.gov (United States)

    Westerhoff, Rogier; Huizinga, Jan; Kleuskens, Marco; Burren, Richard; Casey, Simon

    2010-12-01

    Reliable and timely information is essential for appropriate flood management. This article describes a probabilistic method to assess flood extent from SAR data. The article also addresses the derivation of flood levels and flood depth based on probabilistic flood extents and SRTM. The methods are tested on Envisat ASAR images in a hydrological open standard IT platform (Delft-FEWS). Providing flood extent maps in terms of probabilities using multiple angle data offers advantages for operational purposes, like major improvement of revisit time from 35 to 1-2 days, weighted merging of various data sources (in-situ, optical and SAR) and uncertainty propagation in models. Using medium or high resolution SAR data instead of 1x1 km pixels and using high resolution digital terrain model instead of SRTM data are important recommendations.

  8. Collaboration of the Dutch research program for radioactive waste disposal (OPERA) and TU Delft

    Energy Technology Data Exchange (ETDEWEB)

    Bykov, D.M.; Kloosterman, J.L. [TU Delft (Netherlands). Reactor Inst. Delft; Neeft, E.A.C.; Verhoef, E.V. [COVRA N.V., Nieuwdorp (Netherlands)

    2015-07-01

    awarded research proposals available at www.covra.nl/downloads/o pera. Building up competences and knowledge on radioactive waste management and geological disposal is an important goal of OPERA. To this end OPERA collaborates with TU Delft to develop an academic curriculum for the chair Chemistry of the Nuclear Fuel Cycle in the Master of Science Engineering. Furthermore, the results obtained in OPERA will be presented at a Summer School which is planned to be organized at the end of the Program. TU Delft educates young specialists, bachelor and master students, by research projects on geological disposal at the new actinide laboratory (U and Th). For such purpose the knowledge transfer from other OPERA partners is also foreseen, including students internships and visits of the research facilities. Reactor Institute Delft (RID) is part of the Applied Sciences faculty of TU Delft and houses the Radiation Science and Technology (RST) department. RID operates the Hoger Onderwijs Reactor (HOR), a 2 MW pool- type research reactor in an academic setting. The reactor is used as a source of neutrons and positrons for research purposes, including those of OPERA. It also provides neutrons to a variety of facilities for radioisotope production and neutron activation analysis. OPERA is financed by the Dutch Ministry of Economic Affairs and the public limited liability company Elektriciteits-Produktiemaatschappij Zuid-Nederland (EPZ) and coordinated by COVRA.

  9. Three centuries of protozoology: a brief tribute to its founding father, A. van Leeuwenhoek of Delft.

    Science.gov (United States)

    Corliss, J O

    1975-02-01

    It was exactly 300 years ago this month (August 1974) that the 17th century modest draper from Delft, Holland--Antony van Leeuwenhoek--discovered protozoa. Describing them, often with amazing accuracy considering the optical equipment he was using (simply a home-made "glorified" hand lens), in letters to the Royal Society of London, he established himself, certainly, as the founding father of protozoology. It is particularly appropriate for an assemblage of protozoologists to pay homage to this intrepid "philosopher in little things," a man with an insatiable curiosity about his wee animalcules, on the tricentenary of his discovery of them, since it was an event of such long-lasting significance.

  10. Influence of single nucleotide polymorphisms on thrombin generation in factor V Leiden heterozygotes.

    Science.gov (United States)

    Segers, O; Simioni, P; Tormene, D; Castoldi, E

    2014-03-03

    Carriership of the factor V (FV) Leiden mutation increases the risk of venous thromboembolism (VTE) ~4-fold, but the individual risk of each FV Leiden carrier depends on several co-inherited risk and protective factors. Under the hypothesis that thrombin generation might serve as an intermediate phenotype to identify genetic modulators of VTE risk, we enrolled 188 FV Leiden heterozygotes (11 with VTE) and determined the following parameters: thrombin generation in the absence and presence of activated protein C (APC); plasma levels of prothrombin, factor X, antithrombin, protein S and tissue factor pathway inhibitor; and the genotypes of 24 SNPs located in the genes encoding these coagulation factors and inhibitors. Multiple regression analysis was subsequently applied to identify the (genetic) determinants of thrombin generation. The endogenous thrombin potential (ETP) showed a striking inter-individual variability among different FV Leiden carriers and, especially when measured in the presence of APC, correlated with VTE risk. Several SNPs in the F2 (rs1799963, rs3136516), F10 (rs693335), SERPINC1 (rs2227589), PROS1 (Heerlen polymorphism) and TFPI (rs5940) genes significantly affected the ETP-APC and/or the ETP+APC in FV Leiden carriers. Most of these SNPs have shown an association with VTE risk in conventional epidemiological studies, suggesting that the genetic dissection of thrombin generation leads to the detection of clinically relevant SNPs. In conclusion, we have identified several SNPs that modulate thrombin generation in FV Leiden heterozygotes. These SNPs may help explain the large variability in VTE risk observed among different FV Leiden carriers.

  11. Nuclear themes. Farewell speech of professor Hugo van Dam at the Delft University of Technology; Kernthema's. Afscheidsrede van professor Hugo van Dam van de Technische Universiteit Delft

    Energy Technology Data Exchange (ETDEWEB)

    Van Dam, H. [Faculteit der Technische Natuurwetenschappen, Technische Universiteit Delft, Delft (Netherlands)

    2003-07-01

    Farewell speech of a professor of theoretical and applied physics (reactor physics) at the Faculty of Technical Sciences of the Delft University of Technology, September 5, 2003. An overview is given of the experiences of the author in his career with respect to nuclear energy, focusing on the use of nuclear energy in weapons, ship propulsion and reactors. [Dutch] Rede uitgesproken bij het afscheid als hoogleraar in the theoretische en toegepaste natuurkunde (reactorfysica) aan de Faculteit der Technische Natuurwetenschappen van de Technische Universiteit Delft op vrijdag 5 september 2003. Er wordt een overzicht gegeven van de ervaringen van de auteur in zijn carriere m.b.t. kernenergie, met speciale aandacht voor het gebruik van kernenergie in wapens, scheepsvoortstuwing, en kerncentrales.

  12. HI shells in the Leiden/Argentina/Bonn HI survey

    Science.gov (United States)

    Ehlerová, S.; Palouš, J.

    2013-02-01

    Aims: We analyse the all-sky Leiden/Argentina/Bonn HI survey, where we identify shells belonging to the Milky Way. Methods: We used an identification method based on the search of continuous regions of a low brightness temperature that are compatible with given properties of HI shells. Results: We found 333 shells in the whole Galaxy. The size distribution of shells in the outer Galaxy is fitted by a power law with the coefficient of 2.6 corresponding to the index 1.8 in the distribution of energy sources. Their surface density decreases exponentially with a scale length of 2.8 kpc. The surface density of shells with radii ≥100 pc in the solar neighbourhood is ~4 kpc-2 and the 2D porosity is ~0.7. Tables A.1 and A.2 are only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/550/A23

  13. Heterogeneous ethnic distribution of the factor v leiden mutation

    Directory of Open Access Journals (Sweden)

    Franco Rendrik F.

    1999-01-01

    Full Text Available Inherited resistance to activated protein C caused by the factor V Leiden (FVL mutation is the most common genetic cause of venous thrombosis yet described, being found in 20-60% of patients with venous thrombophilia. A relationship between the FVL mutation and an increased predisposition to arterial thrombosis in young women was recently reported. We assessed the prevalence of the FVL mutation in 440 individuals (880 chromosomes belonging to four different ethnic groups: Caucasians, African Blacks, Asians and Amerindians. PCR amplification followed by MnlI digestion was employed to define the genotype. The FVL mutation was found in a heterozygous state in four out of 152 Whites (2.6%, one out of 151 Amerindians (0.6%, and was absent among 97 African Blacks and 40 Asians. Our results confirm that FVL has a heterogeneous distribution in different human populations, a fact that may contribute to geographic and ethnic differences in the prevalence of thrombotic diseases. In addition, these data may be helpful in decisions regarding the usefulness of screening for the FVL mutation in subjects at risk for thrombosis.

  14. Prevalence of factor Ⅴ Leiden and prothrombin G20210A in patients with gastric cancer

    Institute of Scientific and Technical Information of China (English)

    Sandra Battistelli; Massimo Stefanoni; Alberto Genovese; Aurelio Vittoria; Roberto Cappelli; Franco Roviello

    2006-01-01

    AIM: To analyze the prevalence of the two commonest thrombophilic mutations, factor Ⅴ Leiden and prothrombin G20210A, in patients with gastric cancer.METHODS: One hundred and twenty-one patients with primary gastric carcinoma and 130 healthy subjects,comparable for age and sex, were investigated. Factor Ⅴ Leiden was detected by using polymerase chain reaction and restriction enzyme digestion, and prothrombin G20210A gene mutation by allele-specific PCR.RESULTS: Among the 121 cancer patients, factor Ⅴ Leiden was found in 4 cases (GA genotype: 3.3%) and prothrombin G20210A in 10 cases (GA genotype: 8.3%).Of the 130 control subjects, factor Ⅴ Leiden was detected in 6 cases (GA genotype: 4.6%) and prothrombin G20210A in 8 cases (GA genotype: 6.1%). No double heterozygous carriers of both mutations were found in either group. The prevalence of both factor Ⅴ Leiden and prothrombin G20210A variant was not statistically different between the cancer patients and the healthy subjects.CONCLUSION: Our study suggests that, in gastric cancer, the risk factors of thrombophilic cancer state are on acquired rather than on a genetic basis and that prothrombin G20210A does not seem to be a cofactor in gastric cancer pathogenesis.

  15. Recurrent venous thromboembolism in a patient with heterozygous factor v leiden mutation.

    Science.gov (United States)

    White, C Whitney; Thomason, Angela R; Prince, Valerie

    2014-09-01

    To report a patient case identifying risk for recurrent venous thromboembolism (VTE) associated with heterozygous Factor V Leiden mutation. A 54-year-old Caucasian male was diagnosed with heterozygous Factor V Leiden mutation in 2008 after experiencing a deep vein thrombosis (DVT) and bilateral pulmonary embolism. The patient was treated appropriately and started on anticoagulation therapy with warfarin through an anticoagulation management clinic. After approximately 17 months of warfarin therapy without incident, warfarin was discontinued. Within 2 months after discontinuation of anticoagulation therapy, the patient experienced his second DVT and left pulmonary artery embolus. The risk of recurrent venous thromboembolism (VTE) in patients with heterozygous Factor V Leiden mutation is documented as an approximate 1.4-fold increase compared to patients without thrombophilia. However, the risk increases dramatically when nonreversible (age) or reversible risk factors (obesity, smoking, and long air flights) are present in this population. Based on recent literature, heterozygous Factor V Leiden mutation exponentially increases the risk of recurrent VTE, especially in the presence of other risk factors. Health care providers should complete a comprehensive review of the patients' other risk factors when deciding on duration of anticoagulation therapy for patients with positive heterozygous Factor V Leiden mutation.

  16. Cerebral venous and sinus thrombosis and thrombophilic mutations in Western Iran: association with factor V Leiden.

    Science.gov (United States)

    Rahimi, Zohreh; Mozafari, Hadi; Bigvand, Amir Hossein Amiri; Doulabi, Reza Mohammad; Vaisi-Raygani, Asad; Afshari, Dariush; Razazian, Nazanin; Rezaei, Mansour

    2010-08-01

    The present study aimed at investigating the prevalence of factor V Leiden G1691A, prothrombin G20210A, and MTHFR C677T in cerebral venous and sinus thrombosis (CVST) patients and their possible association with CVST in Western Iran. A total of 24 CVST patients with the mean age of 37.1 +/- 11.7 years and 100 sex- and age-matched healthy individuals from Kermanshah Province of Iran with ethnic background of Kurd were studied for factor V Leiden G1691A, prothrombin G20210A and MTHFR C677T by PCR-RFLP method using Mnl I, Hind III, and Hinf I restriction enzymes, respectively. Prevalence of factor V Leiden was 16.7% in patients and 2% in control group. A significant association was found between factor V Leiden mutation and CVST with odds ratio (OR) of 9.8 (95% confidence intervals [CI] 1.68-57.2, P = .01). No prothrombin G20210A was found among patients. In patients, MTHFR C677T tended to be higher (58.3%) compared to control (44%), OR of 1.8 (95% CI 0.73-4.5, P = .2). Our study for the first time has determined the prevalence of inherited thrombophilia in a homogenous ethnic group of CVST patients and suggests that factor V Leiden, and not the prothrombin gene mutation is a risk factor for CVST in Western Iran.

  17. Both lipolysis and hepatic uptake of VLDL are impaired in transgenic mice coexpressing human apolipoprotein E*3Leiden and human apolipoprotein C1

    NARCIS (Netherlands)

    Jong, M.C.; Dahlmans, V.E.H.; Gorp, P.J.J. van; Breuer, M.L.; Mol, M.J.T.M.; Zee, A. van der; Frants, R.R.; Hofker, M.H.; Havekes, L.M.

    1996-01-01

    Transgenic mice overexpressing human APOE*3Leiden are highly susceptible to diet-induced hyperlipoproteinemia and atherosclerosis due to a defect in hepatic uptake of remnant lipoproteins. In addition to the human APOE*3Leiden gene, these mice carry the human APOC1 gene (APOE*3Leiden- C1). To

  18. Simulation of water temperature in two reservoirs with Delft3d

    Science.gov (United States)

    Yang, J. Y.; Zhou, L. Y.

    2016-08-01

    The proposeled Guanjingkou and Fengdou reservoir will be constructed at Chongqing city and Muling city in China respectively. The water temperature in the reservoir, in the downstream, and the aquatic ecosystem would be altered by the construction of the reservoirs. This paper simulates the water temperature in the two reservoirs by using the Delft3d z-layer model, which uses the fixed elevation for layers. According to the simulation results, the temperature profile in the reservoirs can be divided into three layers: the upmost epilimnion layer, the beneathed thermocline layer, and the constant tepmerature layer at bottom. The temperature effects can be reduced by measurements of stoplogs gates and mutiple gates, respectively. Based on the simulation results in the wet, nomal, and dry year, the temperature of water released from the stoplogs gates at Guanjingkou reservior can be respectively increased by 5.7°C, 6.8°C, 9.6°C, and 5.5°C in the irrigation season from May to August. The temperature of water released from the mutiple gates at Fengdou reservior can be respectively increased by 7.7 °C, 1.9 °C, 9.5 °C, and 10.1 °C from May to August. The negative impacts from the water with lower temperature on the related ecosystem can be significently alleviated.

  19. Filtration Characterization Method as Tool to Assess Membrane Bioreactor Sludge Filterability—The Delft Experience

    Directory of Open Access Journals (Sweden)

    Maria Lousada-Ferreira

    2014-04-01

    Full Text Available Prevention and removal of fouling is often the most energy intensive process in Membrane Bioreactors (MBRs, responsible for 40% to 50% of the total specific energy consumed in submerged MBRs. In the past decade, methods were developed to quantify and qualify fouling, aiming to support optimization in MBR operation. Therefore, there is a need for an evaluation of the lessons learned and how to proceed. In this article, five different methods for measuring MBR activated sludge filterability and critical flux are described, commented and evaluated. Both parameters characterize the fouling potential in full-scale MBRs. The article focuses on the Delft Filtration Characterization method (DFCm as a convenient tool to characterize sludge properties, namely on data processing, accuracy, reproducibility, reliability, and applicability, defining the boundaries of the DFCm. Significant progress was made concerning fouling measurements in particular by using straight forward approaches focusing on the applicability of the obtained results. Nevertheless, a fouling measurement method is still to be defined which is capable of being unequivocal, concerning the fouling parameters definitions; practical and simple, in terms of set-up and operation; broad and useful, in terms of obtained results. A step forward would be the standardization of the aforementioned method to assess the sludge filtration quality.

  20. Filtration characterization method as tool to assess membrane bioreactor sludge filterability-the delft experience.

    Science.gov (United States)

    Lousada-Ferreira, Maria; Krzeminski, Pawel; Geilvoet, Stefan; Moreau, Adrien; Gil, Jose A; Evenblij, Herman; van Lier, Jules B; van der Graaf, Jaap H J M

    2014-04-30

    Prevention and removal of fouling is often the most energy intensive process in Membrane Bioreactors (MBRs), responsible for 40% to 50% of the total specific energy consumed in submerged MBRs. In the past decade, methods were developed to quantify and qualify fouling, aiming to support optimization in MBR operation. Therefore, there is a need for an evaluation of the lessons learned and how to proceed. In this article, five different methods for measuring MBR activated sludge filterability and critical flux are described, commented and evaluated. Both parameters characterize the fouling potential in full-scale MBRs. The article focuses on the Delft Filtration Characterization method (DFCm) as a convenient tool to characterize sludge properties, namely on data processing, accuracy, reproducibility, reliability, and applicability, defining the boundaries of the DFCm. Significant progress was made concerning fouling measurements in particular by using straight forward approaches focusing on the applicability of the obtained results. Nevertheless, a fouling measurement method is still to be defined which is capable of being unequivocal, concerning the fouling parameters definitions; practical and simple, in terms of set-up and operation; broad and useful, in terms of obtained results. A step forward would be the standardization of the aforementioned method to assess the sludge filtration quality.

  1. Antony van Leeuwenhoek's microscopes and other scientific instruments: new information from the Delft archives.

    Science.gov (United States)

    Zuidervaart, Huib J; Anderson, Douglas

    2016-07-01

    This paper discusses the scientific instruments made and used by the microscopist Antony van Leeuwenhoek (1632-1723). The immediate cause of our study was the discovery of an overlooked document from the Delft archive: an inventory of the possessions that were left in 1745 after the death of Leeuwenhoek's daughter Maria. This list sums up which tools and scientific instruments Leeuwenhoek possessed at the end of his life, including his famous microscopes. This information, combined with the results of earlier historical research, gives us new insights about the way Leeuwenhoek began his lens grinding and how eventually he made his best lenses. It also teaches us more about Leeuwenhoek's work as a surveyor and a wine gauger. A further investigation of the 1747 sale of Leeuwenhoek's 531 single lens microscopes has not only led us to the identification of nearly all buyers, but also has provided us with some explanation about why only a dozen of this large number of microscopes has survived.

  2. Green micro-resistojet research at Delft University of Technology: new options for Cubesat propulsion

    Science.gov (United States)

    Cervone, A.; Zandbergen, B.; Guerrieri, D. C.; De Athayde Costa e Silva, M.; Krusharev, I.; van Zeijl, H.

    2017-03-01

    The aerospace industry is recently expressing a growing interest in green, safe and non-toxic propellants for the propulsion systems of the new generation of space vehicles, which is especially true in the case of Cubesat micro-propulsion systems. Demanding requirements are associated to the future missions and challenges offered by this class of spacecraft, where the availability of a propulsion system might open new possibilities for a wide range of applications including orbital maintenance and transfer, formation flying and attitude control. To accomplish these requirements, Delft University of Technology is currently developing two different concepts of water-propelled micro-thrusters based on MEMS technologies: a free molecular micro-resistojet operating with sublimating solid water (ice) at low plenum gas pressure of less than 600 Pa, and a more conventional micro-resistojet operating with liquid water heated and vaporized by means of a custom designed silicon heating chamber. In this status review paper, the current design and future expected developments of the two micro-propulsion concepts is presented and discussed, together with an initial analysis of the expected performance and potential operational issues. Results of numerical simulations conducted to optimize the design of the heating and expansion slots, as well as a detailed description of the manufacturing steps for the conventional micro-resistojet concept, are presented. Some intended steps for future research activities, including options for thrust intensity and direction control, are briefly introduced.

  3. Johannes Vermeer of Delft [1632-1675] and vision in neuroendoscopy

    Directory of Open Access Journals (Sweden)

    Waleed A Azab

    2014-01-01

    Full Text Available Background: Johannes Vermeer of Delft [1632-1675] was one of the greatest Masters of the Dutch Golden Age who was intensely preoccupied with the behavior of light and other optical effects and was entitled "The Master of Light". He fastidiously attended to the subtleties of visual expression through geometry, composition, and precise mastery of the rules of perspective. It has been our impression that some visual similarity does exist between neuroendoscopic images and some of Vermeer′s paintings. Such a relation could be explained by the fact that optical devices are utilized in producing both types of display. Methods: We reviewed the pertinent medical and art literature, observed some video clips of our endoscopy cases, and inspected digital high resolution images of Vermeer′s paintings in order to elaborate on shared optical phenomena between neuroendoscopic views and Vermeer′s paintings. Results: Specific optical phenomena are indeed shared by Johannes Vermeer′s works and neuroendoscopic vision, namely light and color effects as well as the rules of perspective. Conclusion: From the physical point of view, the possibility that a camera obscura inspired Vermeer′s artistic creation makes the existence of a visual link between his paintings and the endoscopic views of the intracranial cavity comprehensible.

  4. A RARE CASE OF FACTOR V LEIDEN MUTATION COMPLICATING PREGNANCY IN INDIA

    Directory of Open Access Journals (Sweden)

    Chitra

    2016-05-01

    Full Text Available Factor V Leiden mutation (Factor V Leiden is an autosomal dominant haemostatic disorder that predisposes affected persons to venous thromboembolic events (VTE. Although the mutation causing FVL is easily diagnosed using molecular DNA techniques, (1 patients who are heterozygous for this disorder often remain asymptomatic until they develop a concurrent prothrombotic condition. Pregnancy, which may increase an individual woman’s risk of venous thromboembolic events by 5- to 6-fold. (2 Because there are potentially serious effects of FVL for both the mother and the child, and availability of effective treatment strategies, early detection and treatment of this condition is warranted. (3 We are presenting this case in order to emphasise the existence of Factor V Leiden in Indian population and its approach during pregnancy.

  5. Homozygous factor V Leiden mutation in type IV Ehlers-Danlos patient.

    Science.gov (United States)

    Refaat, Marwan; Hotait, Mostafa; Winston, Brion

    2014-03-16

    Ehlers-Danlos syndrome (EDS) is a group of inherited connective tissue disorders caused by collagen synthesis defects. Several hemostatic abnormalities have been described in EDS patients that increase the bleeding tendencies of these patients. This case report illustrates a patient with an unusual presentation of a patient with type IV EDS, platelet δ-storage pool disease and factor V Leiden mutation. Young woman having previous bilateral deep vein thrombosis and pulmonary emboli coexisting with ruptured splenic aneurysm and multiple other aneurysms now presented with myocardial infarction. Presence of factor V Leiden mutation raises the possibility that the infarct was due to acute coronary thrombosis, although coronary artery aneurysm and dissection with myocardial infarction is known to occur in vascular type EDS. This is the first report in the medical literature of factor V Leiden mutation in an EDS patient which made the management of our patient challenging with propensity to both bleeding and clotting.

  6. Homozygous factor V Leiden mutation in type IV Ehlers-Danlos patient

    Science.gov (United States)

    Refaat, Marwan; Hotait, Mostafa; Winston, Brion

    2014-01-01

    Ehlers-Danlos syndrome (EDS) is a group of inherited connective tissue disorders caused by collagen synthesis defects. Several hemostatic abnormalities have been described in EDS patients that increase the bleeding tendencies of these patients. This case report illustrates a patient with an unusual presentation of a patient with type IV EDS, platelet δ-storage pool disease and factor V Leiden mutation. Young woman having previous bilateral deep vein thrombosis and pulmonary emboli coexisting with ruptured splenic aneurysm and multiple other aneurysms now presented with myocardial infarction. Presence of factor V Leiden mutation raises the possibility that the infarct was due to acute coronary thrombosis, although coronary artery aneurysm and dissection with myocardial infarction is known to occur in vascular type EDS. This is the first report in the medical literature of factor V Leiden mutation in an EDS patient which made the management of our patient challenging with propensity to both bleeding and clotting. PMID:24653990

  7. Testosterone, anastrozole, factor V Leiden heterozygosity and osteonecrosis of the jaws.

    Science.gov (United States)

    Pandit, Ramesh S; Glueck, Charles J

    2014-04-01

    Our specific aim is to describe the development of thrombotic osteonecrosis of the jaws after testosterone-anastrozole therapy in a 55-year-old white man subsequently found to have previously undiagnosed factor V Leiden heterozygosity. Before the diagnosis of V Leiden heterozygosity, he was given testosterone gel, 50 mg/day, and on testosterone, serum testosterone (963 ng/dl) and estradiol were high (50 pg/ml). Anastrozole was started, and testosterone was continued. Six months later, osteonecrosis of the jaws was diagnosed. Exogenous testosterone is aromatized to estradiol and estradiol-induced thrombophilia, when superimposed on underlying familial thrombophilia, as in this case, may lead to thrombosis and osteonecrosis. We recommend that before giving testosterone, at a minimum, screening for the factor V Leiden and G20210A mutations, and factor VIII and XI activity be carried out, to avoid unanticipated thrombosis.

  8. Mistaken Identity and Mirror Images: Albert and Carl Einstein, Leiden and Berlin, Relativity and Revolution

    Science.gov (United States)

    van Dongen, Jeroen

    2012-06-01

    Albert Einstein accepted a "special" visiting professorship at the University of Leiden in the Netherlands in February 1920. Although his appointment should have been a mere formality, it took until October of that year before Einstein could occupy his special chair. Why the delay? The explanation involves a case of mistaken identity with Carl Einstein, Dadaist art, and a particular Dutch fear of revolutions. But what revolutions was one afraid of? The story of Einstein's Leiden chair throws new light on the reception of relativity and its creator in the Netherlands and in Germany.

  9. The association of factor V Leiden with various clinical patterns of venous thromboembolism-the factor V Leiden paradox.

    Science.gov (United States)

    Hirmerova, J; Seidlerova, J; Subrt, I

    2014-09-01

    Factor V Leiden (FVL) supposedly carries relatively higher risk of deep vein thrombosis (DVT), compared to the risk of pulmonary embolism (PE). To prove this paradox in a group of patients with various clinical presentation of venous thromboembolism (VTE). We retrospectively evaluated clinical pattern of VTE in patients who had been referred to vascular clinic shortly after an acute VTE event. In FVL positive and FVL negative groups we compared the prevalence of isolated symptomatic DVT (proximal or distal) and symptomatic PE with/without DVT, and, moreover, asymptomatic DVT or PE. Of 575 patients (mean age 57 years, 50.1% women), 120 were FVL positive and those had significantly higher prevalence of isolated symptomatic DVT, compared to symptomatic PE with/without DVT. Proximal DVT location was significantly more frequent in FVL carriers. The prevalence of asymptomatic PE did not differ between the two groups. The rate of asymptomatic DVT tended to be higher in FVL negative group. In a multivariate analysis, we confirmed FVL to be positively associated with isolated DVT presentation (odds ratio OR 1.757; 95% confidence interval (CI) 1.148-2.690). On the contrary, increasing age and unprovoked nature of VTE event carried a higher risk of symptomatic PE. We confirmed FVL to be significantly associated with isolated symptomatic DVT despite higher prevalence of proximal DVT in FVL carriers. The fact of relatively lower risk of PE in FVL positive patients might have clinical implication. However, mechanisms of FVL paradox remain to be elucidated. © The Author 2014. Published by Oxford University Press on behalf of the Association of Physicians. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  10. Evaluation the frequency of factor V Leiden mutation in pregnant women with preeclampsia syndrome in an Iranian population.

    Science.gov (United States)

    Karimi, Samieh; Yavarian, Majid; Azinfar, Azadeh; Rajaei, Minoo; Azizi Kootenaee, Maryam

    2012-01-01

    Role of genetic factors in etiology of preeclampsia is not confirmed yet. Gene defect frequency varies in different geographic areas as well as ethnic groups. In this study, the role of factor V Leiden mutation in the pathogenesis of preeclampsia syndrome among the pregnant population of northern shore of Persian Gulf in Iran, were considered. Between Jan. 2008 and Dec. 2009, in a nested case control study, pregnant women with preeclampsia (N=198) as cases and healthy (N=201) as controls were enrolled in the study. DNA were extracted from 10 CC peripheral blood and analyzed for presence of factor V Leiden mutation in these subjects. The maternal and neonatal outcomes of pregnancy according to the distribution of factor V Leiden were also compared among cases. In total, 17(8.6%) of cases and 2(1%) of controls showed the factor V Leiden mutation. The incidence of factor V Leiden was typically higher in preeclamptic women than control group (OR: 9.34 %95 CI: 2.12-41.01). There was no difference in incidence rate of preterm deliveryfactor V Leiden mutation. The pregnant women with factor V Leiden mutation are prone for preeclampsia syndrome during pregnancy, but this risk factor was not correlated to pregnancy complications in the studied women.

  11. Subacute Budd-Chiari syndrome associated with polycythemia vera and factor V Leiden mutation

    NARCIS (Netherlands)

    Simsek, S; Verheesen, RV; Haagsma, EB; Lourens, J

    We describe a 48-year-old caucasian woman with a subacute Budd-Chiari syndrome attributed to the presence of polycythaemia vera, heterozygosity for the factor V Leiden mutation and the use of an oral contraceptive pill. Two diagnostic pitfalls were encountered. First, on CT scanning of the abdomen

  12. Olmesartan and pravastatin additively reduce development of atherosclerosis in APOE*3Leiden transgenic mice

    NARCIS (Netherlands)

    Hoorn, J.W.A. van der; Kleemann, R.; Havekes, L.M.; Kooistra, T.; Princen, H.M.G.; Jukema, J.W.

    2007-01-01

    AIM: This study was designed to investigate the effect of the angiotensin II receptor blocker olmesartan alone, or in combination with standard treatment with a statin, pravastatin, on atherosclerosis development in APOE*3Leiden transgenic mice. METHODS AND RESULTS: Four groups of 15 mice received

  13. Increased sperm count may account for high population frequency of factor V Leiden

    NARCIS (Netherlands)

    D.M. Cohn; S. Repping; H.R. Büller; J.C.M. Meijers; S. Middeldorp

    2010-01-01

    Background: Factor V Leiden (FVL) increases the risk of venous thrombosis and pregnancy loss in carriers. Nevertheless, this relatively old mutation is prevalent in Caucasion populations, which could be explained by positive selection pressure. Men with FVL have previously been found to have higher

  14. Verzeichnis der Echiuridae, Sipunculidae und Priapulidae des Naturhistorichen Reichsmuseums in Leiden

    NARCIS (Netherlands)

    Stiasny, G.

    1930-01-01

    Die vorliegende Mitteilung ist das Ergebnis der Bearbeitung zumeist älteren Materiales an Echiuriden, Sipunculiden und Priapuliden des Naturhistorischen Reichs-Museums in Leiden. Obwohl die Sammlung nach Anzahl der Exemplare nicht umfangreich ist, zeigt sie einen überraschenden Reichtum an Formen

  15. Increased risk for fetal loss in carriers of the factor V Leiden mutation

    NARCIS (Netherlands)

    Meinardi, [No Value; Middeldorp, S; de Kam, PJ; Koopman, MMW; van Pampus, ECM; Hamulyak, K; Prins, MH; Buller, HR; van der Meer, J

    1999-01-01

    Background: An increased risk for fetal loss caused by placental thrombosis is probable in carriers of the factor V Leiden mutation but has not been demonstrated consistently in previous studies. Objective: To determine the overall risk for fetal loss and the separate risks for miscarriage and still

  16. Types of Recent Cephalopoda in the National Museum of Natural History, Leiden

    NARCIS (Netherlands)

    Roeleveld, M.A.C.; Goud, J.; Gleadall, I.C.

    2003-01-01

    A list is given of five name-bearing taxa in the collection of the National Museum of Natural History in Leiden. There is also a brief discussion of purported type material and a summary of new information updating the types list of Sweeney & Roper, 1998.

  17. Type specimens of Maastrichtian fossils in the National Museum of Natural History, Leiden

    NARCIS (Netherlands)

    Leloux, J.

    2002-01-01

    The type specimens of Maastrichtian invertebrate fossils from Limburg, The Netherlands, present in the National Museum of Natural History, Leiden, are listed. The Upper Cretaceous plant type specimens from Limburg of Miquel that were once part of the Staring collection present in the Palaeobotanical

  18. Plasma hepcidin levels and anemia in old age. The Leiden 85-Plus Study

    NARCIS (Netherlands)

    Elzen, W.P. den; Craen, A.J. de; Wiegerinck, E.T.G.; Westendorp, R.G.J.; Swinkels, D.W.; Gussekloo, J.

    2013-01-01

    Hepcidin, an important regulator of iron homeostasis, is suggested to be causally related to anemia of inflammation. The aim of this study was to explore the role of plasma hepcidin in anemia among older persons from the general population. The Leiden 85-Plus Study is a population-based study of

  19. [Adrenal hemorrhage in a newborn with factor V Leiden--a clinical case].

    Science.gov (United States)

    Vulkova, A; Kovacheva, K; ionov, M; Rosmanova, R; Atanasova, V; Slavkova, N; Ivanov, P

    2009-01-01

    Neonatal adrenal hemorrhage is frequently associated with birth trauma, perinatal asphyxia, septicemia, coagulation defects and thromboembolism. We report a case of bilateral adrenal hemorrhage in newborn baby with perinatal asphyxia. Ultrasound findings of subacute adrenal hemorrhage draw attention to its presumable antenatal genesis. DNA analysis for thrombophilic mutations identifies factor V Leiden.

  20. Verzeichnis der Echiuridae, Sipunculidae und Priapulidae des Naturhistorichen Reichsmuseums in Leiden

    NARCIS (Netherlands)

    Stiasny, G.

    1930-01-01

    Die vorliegende Mitteilung ist das Ergebnis der Bearbeitung zumeist älteren Materiales an Echiuriden, Sipunculiden und Priapuliden des Naturhistorischen Reichs-Museums in Leiden. Obwohl die Sammlung nach Anzahl der Exemplare nicht umfangreich ist, zeigt sie einen überraschenden Reichtum an Formen ga

  1. The association of factor V leiden mutation with recurrent pregnancy loss.

    Science.gov (United States)

    Kashif, Sumreen; Kashif, Muhammad Ali; Saeed, Anjum

    2015-11-01

    To determine the association of factor V Leiden mutation with recurrent pregnancy loss. The case-control study was conducted at the Department of Haematology, Armed Forces Institute of Pathology, Rawalpindi, Pakistan, from January to June 2012, and comprised women of 18 to 45 years of age who had a history of recurrent pregnancy loss, and controls with no history of pregnancy loss. All the subjects belonged to Punjabi ethnic group. Three ml blood was taken from cases and controls and deoxyribonucleic acid was extracted. In order to identify Factor V Leiden mutation, polymerase chain reaction method was utilised combined with the amplification refractory mutation system. Data was analysed using SPSS 17. Of the 112 subjects, 56(50%) were in each of the two groups. The presence of factor V Leiden mutation among the cases was 3(5.4%) while it was absent among the controls. The mutation was significantly associated with recurrent pregnancy loss (p=0.017).Recurrent pregnancy loss was higher in cases than controls (p=0.001). Factor V Leiden mutation, Recurrent pregnancy loss, PCR (Polymerase chain reaction).

  2. Coexistence of hypofibrinogenemia and factor V Leiden mutation: is the balance shifted to thrombosis?

    Science.gov (United States)

    Miljić, Predrag; Nedeljkov-Jančić, Ružica; Zuvela, Marinko; Subota, Vesna; Dorđević, Valentina

    2014-09-01

    Congenital hypofibrinogenemia and afibrinogenemia are usually associated with an increased risk of bleeding, but occurrence of arterial or venous thrombosis has also been reported in individuals with fibrinogen deficiency. This study reports on a 25-year-old patient with hypofibrinogenemia (fibrinogen 0.6 g/l) and congenital thrombophilia due to heterozygous factor V Leiden mutation who developed spontaneous deep-vein thrombosis (DVT) in the right lower extremity. Regardless of hypofibrinogenemia, he was receiving anticoagulant therapy over 6 months, with no occurrence of bleeding. His father is also a heterozygous carrier of factor V Leiden, but with normal fibrinogen level and he remained asymptomatic despite having experienced surgery in the past. This case, as well as data from literature, suggests that risk of thrombosis in carriers of factor V Leiden mutation is not counterbalanced by moderate congenital hypofibrinogenemia, and that antithrombotic prophylaxis should not be omitted in high-risk situations for occurrence of thrombosis in patients with coinheritance of hypofibrinogenemia and factor V Leiden mutation.

  3. The risk of mortality and the factor V Leiden mutation in a population-based cohort

    NARCIS (Netherlands)

    Heijmans, B.T.; Westendorp, R.G.J.; Knook, D.L.; Kluft, C.; Slagboom, P.E.

    1998-01-01

    The factor V Leiden mutation (conferring resistance to activated protein C) has been implicated in the risk of arterial thrombosis and is a well-established risk factor for venous thrombosis especially in the elderly. We studied whether the disease association of the factor V mutation is reflected

  4. Pneumococcal meningitis and endocarditis in an infant: possible improved survival with factor V Leiden mutation.

    Science.gov (United States)

    Mohapatra, Sitikant; Doulah, Assaf; Brown, Elspeth

    2017-08-12

    Streptococcus pneumoniae infections continue to remain associated with high morbidity and mortality. Although the incidence of invasive meningeal and/or lung disease are not uncommon, Streptococcus pneumoniae endocarditis is rare especially in healthy pediatric population. New studies have suggested a strong association between factor V leiden (FVL) mutation and favorable outcomes in critically ill children. A healthy 10 month old presented with sepsis and meningeal signs, was later confirmed to have Streptococcus pneumoniae meningitis and endocarditis. She was found to have factor V leiden mutation and made a complete recovery despite initial complications. Presence of factor V leiden mutation in critically ill children with severe septicaemia possibly contributes to better outcomes. What is known: • Mortality and morbidity remain high with invasive pneumococcal disease. • Pneumococcal endocarditis is rare in healthy pediatric population and results in significant morbidity and mortality What is new: • New studies have suggested a strong association between factor V leiden (FVL) mutation and favorable outcomes in critically ill children. • The presence of factor V mutation in children with extensive invasive pneumococcal disease possibly contributes to a better outcome.

  5. Cerebral venous thrombosis following spinal surgery in a patient with Factor V Leiden mutation.

    Science.gov (United States)

    Yılmaz, Baran; Ekşi, Murat Şakir; Akakın, Akın; Toktaş, Zafer Orkun; Demir, Mustafa Kemal; Konya, Deniz

    2016-08-01

    Cerebral venous thrombosis is a devastating event leading to high mortality and morbidity rates. We present a case of cerebral venous thrombosis that occurred following spinal surgery in a patient with Factor V Leiden mutation and G1691A heterozygosity. Possible prevention and treatment strategies have been discussed.

  6. Factor V Leiden mutation in relation to fecundity and miscarriage in women with venous thrombosis

    NARCIS (Netherlands)

    Dunne, F.M.; Doggen, Catharina Jacoba Maria; Heemskerk, M.; Rosendaal, F.R.; Helmerhorst, F.M.

    2005-01-01

    BACKGROUND: Factor V Leiden mutation (Arg506Gln) increases the likelihood of venous thrombosis; it may also have a positive effect through facilitation of embryo implantation. This may manifest itself as a reduced time to pregnancy (increased fecundity) and fewer miscarriages in the first trimester.

  7. The finger of God : anatomical practice in 17th century Leiden

    NARCIS (Netherlands)

    Huisman, Tijs

    2008-01-01

    A description of 17th century anatomical activity at the major Dutch university in a cultural context This study offers a history of the Leiden anatomical theatre in the first century of its existence; who were the scientists working there in the 17th century, the Dutch Golden Age. What was the mot

  8. Orthopterological notes I : On the Lesini of the Leiden Museum (Tettigoniidae, Copiphorinae)

    NARCIS (Netherlands)

    Jong, de C.

    1942-01-01

    When rearranging a part of the collections of Orthoptera in the Rijksmuseum van Natuurlijke Historie at Leiden I found a number of specimens belonging to this tribus, which by former authors is considered as a separate subfamily with the name Eumegalodontinae (Kirby, 1906, p. 289; Caudell, 1927, p.

  9. Plasma hepcidin levels and anemia in old age. The Leiden 85-Plus Study

    NARCIS (Netherlands)

    Elzen, W.P. den; Craen, A.J. de; Wiegerinck, E.T.G.; Westendorp, R.G.J.; Swinkels, D.W.; Gussekloo, J.

    2013-01-01

    Hepcidin, an important regulator of iron homeostasis, is suggested to be causally related to anemia of inflammation. The aim of this study was to explore the role of plasma hepcidin in anemia among older persons from the general population. The Leiden 85-Plus Study is a population-based study of 85-

  10. Plasma hepcidin levels and anemia in old age. The Leiden 85-Plus Study

    NARCIS (Netherlands)

    Elzen, W.P. den; Craen, A.J. de; Wiegerinck, E.T.G.; Westendorp, R.G.J.; Swinkels, D.W.; Gussekloo, J.

    2013-01-01

    Hepcidin, an important regulator of iron homeostasis, is suggested to be causally related to anemia of inflammation. The aim of this study was to explore the role of plasma hepcidin in anemia among older persons from the general population. The Leiden 85-Plus Study is a population-based study of 85-

  11. Paul Ehrenfest's rough road to Leiden: a physicist's search for a position, 1904-1912

    NARCIS (Netherlands)

    Huijnen, P.; Kox, A.J.

    2007-01-01

    Paul Ehrenfest (1880-1933) received his Ph.D. degree at the University of Vienna in 1904 and moved with his wife and young daughter to St. Petersburg in 1907, where he remained until he succeeded Hendrik Antoon Lorentz (1853-1928) in the chair of theoretical physics at the University of Leiden in 19

  12. Mistaken Identity and Mirror Images: Albert and Carl Einstein, Leiden and Berlin, Relativity and Revolution

    NARCIS (Netherlands)

    van Dongen, J.A.E.F.

    2012-01-01

    Albert Einstein accepted a “special” visiting professorship at the University of Leiden in the Netherlands in February 1920. Although his appointment should have been a mere formality, it took until October of that year before Einstein could occupy his special chair. Why the delay? The explanation i

  13. Subacute Budd-Chiari syndrome associated with polycythemia vera and factor V Leiden mutation

    NARCIS (Netherlands)

    Simsek, S; Verheesen, RV; Haagsma, EB; Lourens, J

    2000-01-01

    We describe a 48-year-old caucasian woman with a subacute Budd-Chiari syndrome attributed to the presence of polycythaemia vera, heterozygosity for the factor V Leiden mutation and the use of an oral contraceptive pill. Two diagnostic pitfalls were encountered. First, on CT scanning of the abdomen t

  14. Type specimens of amphibians in the National Museum of Natural History, Leiden, The Netherlands

    NARCIS (Netherlands)

    Gasso Miracle, M.E.; Hoek Ostende, van den L.W.; Arntzen, J.W.

    2007-01-01

    The amphibian type specimens held in the National Museum of Natural History in Leiden are listed. A total of 775 type specimens representing 143 taxon names were encountered. The list provides the original name, the original publication date, pagination and illustrations, current name, type locality

  15. Die Scyphomedusen-Sammlung des Naturhistorischen Reichsmuseums in Leiden : II. Stauromedusae, Coronatae, Semaeostomeae

    NARCIS (Netherlands)

    Stiasny, G.

    1919-01-01

    ALLGEMEINER TEIL. Die Bearbeitung der umfangreichen Scyphomedusen-Sammlung im Rijksmuseum van Natuurlijke Historie in Leiden ist nunmehr so weit vorgeschritten, dass sich eine Übersicht über dieselbe gewinnen lässt. In einer früheren Mitteilung wurde über die Cubomedusen berichtet, eine weitere zur

  16. Factor V leiden mutation in Behcet’s disease and the relationship with clinical manifestations

    Directory of Open Access Journals (Sweden)

    Mowla K

    2010-05-01

    Full Text Available "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: Behcet's disease (BD is a multisystemic inflammatory disease with unknown origin characterized by recurrent oral aphtous ulcers, genital, ocular and skin lesions. A single point mutation 1691G to A in the factor V gene increases the risk of venous thrombosis. This study designed to determine factor V Leiden mutation in Behcet's disease, and to find out it's relationship with the clinical manifestations in Khuzestan province, Iran. "n"nMethods: One hundred patients with Behcet's Disease (44 males and 56 females based on international diagnostic criteria and 70 healthy subjects were included in the study. Patients and controls were tested for the presence of factor V Leiden mutation using polymerase chain reaction method."n"nResults: The prevalence of factor V Leiden mutation was significantly higher in BD (10 out of 100, 10% compared with healthy control subjects (1 out of 70, 1.4%, (p=0.025. Vascular lesions in this study were deep vein thrombosis (DVT (7%, subcutaneous thrombophlebitis (5%, stroke (1% and retinal vasculitis (39%. It was found that there was no association between venous thrombosis and the factor V Leiden mutation in Khuzestanian patients. Also, no association between

  17. Factor V Leiden is associated with increased sperm count.

    Science.gov (United States)

    van Mens, T E; Joensen, U N; Bochdanovits, Z; Takizawa, A; Peter, J; Jørgensen, N; Szecsi, P B; Meijers, J C M; Weiler, H; Rajpert-De Meyts, E; Repping, S; Middeldorp, S

    2017-09-12

    Is the thrombophilia mutation factor V Leiden (FVL) associated with an increased total sperm count? Carriers of FVL have a higher total sperm count than non-FVL-carriers, which could not be explained by genetic linkage or by observations in a FVL-mouse model. FVL has a high prevalence in Caucasians despite detrimental health effects. Carriers have been shown to have higher fecundity, which might partly explain this evolutionary paradox. We determined FVL status in two cohorts (Dutch, n = 627; Danish, n = 854) of consecutively included men without known causes for spermatogenic failure, and performed an individual patient data meta-analysis of these two cohorts together with one previously published (Dutch, n = 908) cohort. We explored possible biological underpinnings for the relation between sperm count and FVL, by use of a FVL-mouse model and investigations of genetic linkage. Participants were male partners of subfertile couples (two Dutch cohorts) and young men from the general population (Danish cohort): FVL carrier rate was 4.0%, 4.6% and 7.3%, respectively. There were differences in smoking, abstinence time and age between the cohorts. We corrected for these in the primary analysis, which consisted of a mixed linear effects model, also incorporating unobjectified population differences. In public haplotype data from subjects of European descent, we explored linkage disequilibrium of FVL with all known single nucleotide polymorphisms in a 1.5 MB region around the F5 gene with an R2 cutoff of 0.8. We sequenced exons of four candidate genes hypothesized to be linked to FVL in a subgroup of FVL carriers with extreme sperm count values. The animal studies consisted of never mated 15-18-week-old C57BL/J6 mice heterozygous and homozygous for FVL and wild-type mice. We compared spermatogenesis parameters (normalized internal genitalia weights, epididymis sperm content and sperm motility) between FVL and wild-type mice. Human FVL carriers have a higher total sperm

  18. Annotated catalogue of recent Echinoderm type specimens in the collection of the Rijksmuseum van Natuurlijke Historie at Leiden

    NARCIS (Netherlands)

    Jangoux, M.; Ridder, de C.

    1987-01-01

    The Leiden Museum houses 82 type specimens of recent echinoderms representing 59 nominal species, one nominal subspecies and one variety. Each species, subspecies or variety is presented (original name, current status, Museum characteristics, brief bibliography, and eventual remarks).

  19. Venous thrombosis with both heterozygous factor V Leiden (R507Q) and factor II (G20210A) mutations.

    Science.gov (United States)

    Bhaijee, Feriyl; Jordan, Brenda; Pepper, Dominique J; Leacock, Rodney; Rock, William A

    2012-01-01

    Both hereditary and acquired factors increase the risk of venous thromboembolism, thus the clinical management of affected patients involves evaluation of genetic factors that predispose to hypercoagulability. Factor V Leiden (R507Q) and factor II (prothrombin) mutation (G20210A) are the two most common inherited hypercoagulability disorders among populations of European origin. Both factor V Leiden and factor II mutation (G20210A) represent gain-of-function mutations: factor V Leiden causes resistance to activated protein C, and factor II mutation (G20210A) results in higher levels of plasma prothrombin. Herein, we present an uncommon case of combined factor V Leiden mutation (R507Q) and factor II mutation (G20210A), and discuss the prevalence and features of each entity, as well as their role in the clinical management of affected patients.

  20. Sneddon Syndrome with Factor V Leiden, Methylene Tetrahydrofolate Reductase and FMF Gene Mutations

    Directory of Open Access Journals (Sweden)

    Murat Terzi

    2010-03-01

    Full Text Available Sneddon syndrome (SNS, characterized by livedo racemosa and stroke, is a rare disease, especially in young adults. Livedo racemosa are large lesions, widespread on the extremities and the body, that are violet-colored and have a good appearance and ambiguous limits. A 33-years-old female presented to our clinic for headache. She had a two-year history of blue-purple skin marks on her body and legs. The skin lesions were consistent with livedo racemosa. She had experienced right hemiparesis according to her medical history. Factor V Leiden (G1691A mutation was heterozygote-positive. Methylenetetrahydrofolate reductase (MTHFR C677T and FMF gene (MEFV V726A mutations were determined. SNS is the cause of stroke, rarely seen in young adults. We considered this case to be of value since it is the first SNS case having factor V Leiden, MTHFR and MEFV mutations concomitantly.

  1. A Successful Mother and Neonate Outcome for a Woman with Essential Thrombocytosis and FV Leiden Heterozygosity

    Directory of Open Access Journals (Sweden)

    Marianna Politou

    2016-01-01

    Full Text Available Essential thrombocytosis (ET and FV Leiden heterozygosity represent an acquired and hereditable hypercoagulable state, respectively. An uncommon case of coexistence of ET and FV Leiden heterozygosity in a 36-year-old pregnant woman and her successful pregnancy outcome is described. She was considered to be at high risk of thrombosis during her pregnancy and she was treated with both prophylactic dose of LMWH and aspirin daily throughout her pregnancy and for a 6-week period postpartum. The efficacy of the anticoagulation treatment was monitored in various time points not only by measuring anti-Xa levels and D-Dimers but also with new coagulation methods such as rotation thromboelastometry and multiplate. Global assessment of coagulation using additional newer laboratory tests might prove useful in monitoring coagulation pregnancies at high risk for thrombosis.

  2. [Livedoid vasculopathy with heterozygous factor V Leiden mutation and sticky platelet syndrome].

    Science.gov (United States)

    Lewerenz, V; Burchardt, T; Büchau, A; Ruzicka, T; Megahed, M

    2004-04-01

    A 64-year-old male patient presented with painful ulcerations and livedo racemosa of both lower limbs. He had a history of cerebral and myocardial infarctions. Dermatohistologic findings and laboratory tests of the patient's coagulation system revealed the diagnosis of livedoid vasculopathy with heterozygous factor V Leiden mutation and sticky platelet syndrome type II. Systemic treatment with acetylsalicylic acid and heparin as well as topical therapy with disinfectant and granulation-inducing agents resulted in improvement of the skin lesions.

  3. Regional block anesthesia in a patient with factor V Leiden mutation and axillary artery occlusion

    OpenAIRE

    Ozdemir, haluk

    2011-01-01

    Kerem Erkalp1, Mevlut Comlekci1, Bekir Inan2, Gokcen Basaranoglu1, Haluk Ozdemir1, Leyla Saidoglu11Department of Anaesthesiology and Reanimation, Vakif Gureba Hospital, Istanbul, Turkey; 2Department of Vascular Surgery, Vakif Gureba Hospital, Istanbul, TurkeyAbstract: Anesthetic management of patients with coagulation disorders presents safety and technical challenges. This case describes a 58-year-old woman with factor V Leiden mutation who required distal saphenous vein harvest and axillo-b...

  4. Paternal factor V Leiden and recurrent pregnancy loss: a new concept behind fetal genetics?

    Science.gov (United States)

    Udry, S; Aranda, F M; Latino, J O; de Larrañaga, G F

    2014-05-01

    In up to 50% of couples affected by recurrent pregnancy loss, no identifiable cause is established. Fetal and maternal factors may be equally important in the establishment and maintenance of the placental/maternal arteriovenous anastomoses. Therefore,the inheritance of thrombophilia-related genes may be an important factor in the pathophysiology of recurrent pregnancy loss. Most of the research on recurrent pregnancy loss and thrombophilia has focused on maternal factors, but little is known about the paternal contribution. On that basis, we studied the association between inherited paternal thrombophilias and recurrent pregnancy loss in a narrowly selective group of 42 Argentine males from couples that presented without any known risk factors for recurrent pregnancy loss. The genotypic distributions of factor (F) V Leiden and prothrombin G20210A among cases were compared with those from a reference group composed of 200 Argentine men. We found a significant difference in the distribution of FV Leiden between both groups (16.7% vs. 3.0%), but no difference was found in the distribution of prothrombin G20210A (2.4% vs.2.0%). Those couples with paternal FV Leiden carriage would be six times more likely to experience recurrent pregnancy loss despite no other apparent cause (OR = 6.47; 95% CI, 2.06–20.39). We found evidence of an association between the paternal carriage of FV Leiden and the predisposition to recurrent pregnancy loss, thereby supporting the hypothesis that genetic contributions from both parents are essential factors in the development of this obstetric disorder.

  5. Meta-analysis of factor V Leiden and prothrombin G20210A polymorphism in migraine.

    Science.gov (United States)

    Lippi, Giuseppe; Mattiuzzi, Camilla; Cervellin, Gianfranco

    2015-01-01

    Migraine is a frequent and disabling condition, which exhibits a substantial genetic background and is frequently associated with abnormalities of primary and secondary hemostasis. We performed a systematic literature search and a meta-analysis of available data about the potential associations between migraine and factor V (FV) Leiden or prothrombin (FII) G20210A gene polymorphism. The final number of studies included was 15 (all cross-sectional) about migraine and FV Leiden, and 12 (all cross-sectional) about migraine and FII G20210A polymorphism, with broad inter-study heterogeneity (I², 82 and 85%). The cumulative prevalence of the FV 1691A allele was found to be similar between cases (n = 1450; 4.9%) and controls (n = 3468; 4.7%; P = 0.74). The cumulative prevalence of the FII 20210A allele was also found to be similar between cases (n = 1226; 4.2%) and controls (n = 3144; 4.5%; P = 0.59). Nevertheless, sub-analysis of studies in adults and children revealed that both polymorphisms were not associated with migraine in adults, but FV Leiden and the FII 20210A allele were approximately two-fold more prevalent in children with migraine than in those without. In conclusion, despite migraine exhibits a clear neurovascular origin and is frequently associated with thrombotic disorders, isolate thrombophilic mutations seem to play a negligible pathogenetic role in this condition in adults, whereas the increased prevalence of FV Leiden and the FII 20210A allele in children with migraine deserves further scrutiny.

  6. Prevalence of factor V Leiden mutation in non-European populations.

    Science.gov (United States)

    Pepe, G; Rickards, O; Vanegas, O C; Brunelli, T; Gori, A M; Giusti, B; Attanasio, M; Prisco, D; Gensini, G F; Abbate, R

    1997-02-01

    A difference in the prevalence of venous thromboembolism (TE) in major human groups has been described and an uneven distribution of FV Leiden mutation over the world has recently been reported. We investigated FV Leiden mutation in 584 apparently healthy subjects mostly from populations different from those previously investigated: 170 Europeans (Spanish, Italians), 101 sub-saharan Africans (Fon, Bariba, Berba, Dendi), 115 Asians (Indonesians, Chinese, Tharus), 57 Amerindians (Cayapa), 84 Afroamericans (Rio Cayapa, Viche), and 57 Ethiopians (Amhara, Oromo). The mutation was detected in only 1/115 Asian (Tharu) and in 5/170 Europeans (4 Italians, 1 Spanish). These data confirm that in non-Europeans the prevalence of FV mutation is at least 7 times lower than in Europeans and provide indirect evidence of a low prevalence not only of the FV Leiden gene but also of other genes leading to more severe thrombophilia. Finally, findings from the literature together with those pertaining to this study clearly show a marked heterogeneity among Europeans.

  7. Regional block anesthesia in a patient with factor V Leiden mutation and axillary artery occlusion

    Directory of Open Access Journals (Sweden)

    Kerem Erkalp

    2011-02-01

    Full Text Available Kerem Erkalp1, Mevlut Comlekci1, Bekir Inan2, Gokcen Basaranoglu1, Haluk Ozdemir1, Leyla Saidoglu11Department of Anaesthesiology and Reanimation, Vakif Gureba Hospital, Istanbul, Turkey; 2Department of Vascular Surgery, Vakif Gureba Hospital, Istanbul, TurkeyAbstract: Anesthetic management of patients with coagulation disorders presents safety and technical challenges. This case describes a 58-year-old woman with factor V Leiden mutation who required distal saphenous vein harvest and axillo-brachial bypass to treat axillary artery occlusion. The patient underwent surgery with satisfactory anesthesia using infraclavicular brachial plexus block, thoracic paravertebral block, and unilateral subarachnoid block. These three regional anesthetic interventions were performed in lieu of general anesthesia to minimize risks of thrombotic events, pain, and to decrease recovery time. Despite higher failure rates of regional anesthesia, longer time required for procedures, and added discomforts during surgery, the benefits may outweigh risks for selected high-risk patients, including those with factor V Leiden mutations.Keywords: regional anesthesia, factor V Leiden, pain, vein harvest

  8. Right Ventricular Thrombus in a 36-Year-Old Man with Factor V Leiden.

    Science.gov (United States)

    Hajsadeghi, Shokoufeh; Naghshin, Roozbeh; Hejrati, Maral; Kerman, Scott Reza Jafarian

    2015-01-01

    Factor V Leiden deficiency is the most common hereditary hypercoagulable disease in the United States and involves 5% of the Caucasian population. Up to 30% of patients who present with deep vein thrombosis (DVT) or pulmonary thromboembolism present with this condition. This is a case report of a 36-year-old man who experienced one episode of DVT within the previous year and was admitted to our hospital due to productive coughs and hemoptysis. Paraclinical studies demonstrated a right ventricular thrombus. Additional investigation was done to find the underlying cause. Laboratory tests were positive for Factor V Leiden mutation. Other factors for hypercoagulability states were normal. Given that Factor V Leiden mutation is a life-threatening condition with a relatively high prevalence and considering its thrombogenesis, screening tests are necessary in young patients without obvious reasons for recurrent thrombus formation. It seems that medical noninvasive treatments can be an alternative therapy to surgery when a ventricular thrombus is suspected in these patients.

  9. The fastest bicycle in the world; how a team of Delft students is building a bicycle to break the world speed record

    NARCIS (Netherlands)

    Annega, G.

    2011-01-01

    The Netherlands is a country known for its extensive use of bicycles as a means of everyday transport. However, while it has existed for about two centuries, todays bicycles only differ from those of the 19th century in relatively small details. Human Power Team Delft is building a whole new type of

  10. Advances in computer-aided engineering: CAD/CAM-research at Delft University of Technology. Report of the VF-project CAD/CAM 1989-1994

    NARCIS (Netherlands)

    1994-01-01

    This book contains a collection of articles describing on-going CAD/CAM-research at several engineering faculties at Delft University of Technology. Two main themes covered in this book are 'Conceptual design of complex products' and 'Product modelling and product data exchange'.

  11. Computational Modeling on Concrete, Masonry and Fiber-reinforced Composites : proceedings of the Workshop on 17 – 18 June 2009, Delft, The Netherlands

    NARCIS (Netherlands)

    2009-01-01

    This booklet comprises the extended abstracts to be presented at the Ma(gni)FiCo workshop held in Delft, The Netherlands, on 17-18 June 2009. The spirit of this workshop is to have a small gathering with ample time for lively conversation and interaction among the student and faculty researchers. A

  12. Institute of place making: A project by the chair of Landscape Architecture at the TU Delft. Oerol 2013: Sense of place

    NARCIS (Netherlands)

    Pouderoijen, M.T.; Piccinini, D.

    2013-01-01

    This booklet shows the results of a project developed by TU Delft in a Master elective course offered by the chair of Landscape Architecture: Landscape Architecture ON site, being part of Oerol Festival 2013. The aim of the project was to express the landscape of Terschelling into a temporarily proj

  13. Advances in computer-aided engineering: CAD/CAM-research at Delft University of Technology. Report of the VF-project CAD/CAM 1989-1994

    NARCIS (Netherlands)

    1994-01-01

    This book contains a collection of articles describing on-going CAD/CAM-research at several engineering faculties at Delft University of Technology. Two main themes covered in this book are 'Conceptual design of complex products' and 'Product modelling and product data exchange'.

  14. Technische universiteiten en duurzame ontwikkeling in Afrika: een interfacultair studenten onderzoeksproject voor het 33e lustrumsymposium van de Technische Universiteit Delft

    NARCIS (Netherlands)

    De Vries, G.; Wessels, R.

    2007-01-01

    In 2007, Delft University of Technology celebrates its 165th birthday. This unique occasion is celebrated with several activities revolving around the subject "Sustainable Solutions, with a focus on Africa". For the interdisciplinary course "Technical Universities and Sustainable development of Afri

  15. Evaluation the frequency of factor V Leiden mutation in pregnant women with preeclampsia syndrome in an Iranian population

    Directory of Open Access Journals (Sweden)

    Azadeh Azinfar

    2012-01-01

    Full Text Available Background: Role of genetic factors in etiology of preeclampsia is not confirmed yet.Objective: Gene defect frequency varies in different geographic areas as well as ethnic groups. In this study, the role of factor V Leiden mutation in the pathogenesis of preeclampsia syndrome among the pregnant population of northern shore of Persian Gulf in Iran, were considered.Materials and Methods: Between Jan. 2008 and Dec. 2009, in a nested case control study, pregnant women with preeclampsia (N=198 as cases and healthy (N=201 as controls were enrolled in the study. DNA were extracted from 10 CC peripheral blood and analyzed for presence of factor V Leiden mutation in these subjects. The maternal and neonatal outcomes of pregnancy according to the distribution of factor V Leiden were also compared among cases.Results: In total, 17(8.6% of cases and 2(1% of controls showed the factor V Leiden mutation. The incidence of factor V Leiden was typically higher in preeclamptic women than control group (OR: 9.34 %95 CI: 2.12-41.01. There was no difference in incidence rate of preterm delivery< 37 weeks (OR: 1.23 %95 CI: 0.38-4.02, very early preterm delivery<32 weeks (OR: 1.00 %95 CI: 0.12-8.46, intra uterine fetal growth restriction (IUGR (OR: 1.32 %95 CI: 0.15-11.30 ,and the rate of cesarean section (OR: 0.88 %95 CI: 0.29-2.62 among cases based on the prevalence of factor V Leiden mutation.Conclusion: The pregnant women with factor V Leiden mutation are prone for preeclampsia syndrome during pregnancy, but this risk factor was not correlated to pregnancy complications in the studied women

  16. Involvement of serum retinoids and Leiden mutation in patients with esophageal, gastric, liver, pancreatic, and colorectal cancers in Hungary

    Institute of Scientific and Technical Information of China (English)

    Gyula Mózsik; Zoltán Matus; Béla Melegh; Gy(o)rgy Rumi; András D(o)m(o)t(o)r; Mária Figler; Beáta Gasztonyi; El(o)d Papp; Alajos Pár; Gabriella Pár; József Belágyi

    2005-01-01

    AIM: To analyze the serum levels of retinoids and Leiden mutation in patients with esophageal, gastric, liver,pancreatic, and colorectal cancers.METHODS: The changes in serum levels of retinoids (vitamin A, α- and β-carotene, α- and β-cryptoxanthin,zeaxanthin, lutein) and Leiden mutation were measured by high liquid performance chromatography (HPLC)and polymerase chain reaction (PCR) in 107 patients (70 males/37 females) with esophageal (0/8), gastric (16/5), liver (8/7), pancreatic (6/4), and colorectal (30/21including 9 patients suffering from in situ colon cancer)cancer. Fifty-seven healthy subjects (in matched groups)for controls of serum retinoids and 600 healthy blood donors for Leiden mutation were used.RESULTS: The serum levels of vitamin A and zeaxanthin were decreased significantly in all groups of patients with gastrointestinal (GI) tumors except for vitamin A in patients with pancreatic cancer. No changes were obtained in the serum levels of α- and β-carotene,α- and β-cryptoxanthin, zeaxanthin, lutein in patients with GI cancer. The prevalence of Leiden mutation significantly increased in all groups of patients with GI cancer.CONCLUSION: Retinoids (as environmental factors)are decreased significantly with increased prevalence of Leiden mutation (as a genetic factor) in patients before the clinical manifestation of histologically different (planocellular and hepatocellular carcinoma, and adenocarcinoma) GI cancer.

  17. Clinical characteristics of patients with factor V Leiden or prothrombin G20210A and a first episode of venous thromboembolism. Findings from the RIETE Registry.

    Science.gov (United States)

    Gadelha, Telma; Roldán, Vanessa; Lecumberri, Ramón; Trujillo-Santos, Javier; del Campo, Raquel; Poggio, Renzo; Monreal, Manuel

    2010-10-01

    The clinical characteristics of patients with factor V Leiden or prothrombin G20210A presenting with a first episode of venous thromboembolism (VTE) have not been thoroughly studied. RIETE is an ongoing registry of consecutive patients with acute VTE. We compared the clinical characteristics of patients with factor V Leiden, prothrombin G20210A, or no thrombophilia, at presentation with a first episode of VTE. As of May 2009, 22428 patients had been enrolled with a first episode of VTE. Of these, 345 had factor V Leiden, 261 had prothrombin G20210A, and 2399 tested negative. Sixty-two percent of the VTE episodes in women with factor V Leiden or prothrombin G20210A (40% in men) were associated with an acquired risk factor. Among women, pregnancy or contraceptive use accounted for 63% and 67% of such risk factors. Patients with factor V Leiden presented with pulmonary embolism (PE) less likely than those with prothrombin G20210A (31% vs. 51%; pFactor V Leiden presented with hypoxaemia (Sat O(2) levelsfactor V Leiden or prothrombin G20210A were associated with an acquired risk factor (mostly pregnancy or contraceptive use). Only 4.5% of patients with factor V Leiden presenting with acute PE had hypoxaemia. Copyright © 2010 Elsevier Ltd. All rights reserved.

  18. Molecular characterization of factor V leiden G1691A and prothrombin G20210A mutations in Saudi newborns with stroke.

    Science.gov (United States)

    Gawish, Gihan E-H

    2011-10-01

    This study examined a possible association between the mutations related to Factor V Leiden and Factor II (prothrombin) and stroke in Saudi neonates. A multiplex PCR was established to detect Factor V Leiden G1691A and prothrombin G20210A mutations in 72 neonatal stroke subjects and 70 healthy adult controls with no family history of thromboembolic diseases. The frequency of the homozygous normal genotype (GG) of both genes was found to be significantly lower in the stroke subjects than in the controls (P Factor II heterozygous mutant form (GA) and the homozygous normal Factor V (GG) (P Factor V and the homozygous normal Factor II genotypes (GG) (P = 0.0) than controls. The study concluded that prothrombin and Factor V Leiden may be important risk factors for neonatal stroke in Saudi children.

  19. A case of pulmonary thromboembolism due to coagulation factor V Leiden in Japan ~ usefulness of next generation sequencing~.

    Science.gov (United States)

    Sueta, Daisuke; Ito, Miwa; Uchiba, Mitsuhiro; Sakamoto, Kenji; Yamamoto, Eiichiro; Izumiya, Yasuhiro; Kojima, Sunao; Kaikita, Koichi; Shinriki, Satoru; Hokimoto, Seiji; Matsui, Hirotaka; Tsujita, Kenichi

    2017-01-01

    Because the venous thromboembolisms (VTEs) due to the coagulation factor V R506Q (FV Leiden) mutation is often seen in Caucasians, the VTE onset in Japan has not been reported. A 34-year-old man from north Africa experiencing sudden dyspnea went to a hospital for advice. The patient had pain in his right leg and a high plasma D-dimer level. A contrast-enhanced computed tomography scan revealed a contrast deficit in the bilateral pulmonary artery and in the right lower extremity. The patient was diagnosed with VTE, and anticoagulation therapy was initiated. Our targeted gene panel sequencing revealed that the occurrence of VTE was attributed to a presence of the FV Leiden mutation. This is the first report demonstrating VTE caused by the FV Leiden mutation in Japan.

  20. 基于Delft3D模型的挟沙水流泥沙输运特征分析%Sediment Laden Flow and Sediment Transport Characteristics Analysis Based on Delft3 D Model

    Institute of Scientific and Technical Information of China (English)

    何瑛

    2016-01-01

    灌渠在农业生产中具有重要作用,渠道引水灌溉是我国主要灌溉形式,引水过程势必会伴随引沙。以塔河灌区输水渠道为研究对象,分析灌渠挟沙水流泥沙输特性。利用Delft3 D模型建立二维渠道泥沙输运数学模型,对渠道不同水位、流速、流量下的含沙量进行研究。计算结果表明,塔河灌区输水渠道涨落急流速平均误差小于0.14 m/s,流量峰值误差小于75 m3/s;灌渠流态合理,含沙量计算误差小于0.02 kg/m3;渠床冲淤误差为0.023 m,与测量值相差23%,该数学模型可以较好地反映灌渠挟沙水流泥沙输运情况。%Irrigation plays an important role in agricultural production, water diversion canal irriga-tion is the main form of irrigation, water diversion process will inevitably accompanied by sediment diversion. Using Tahe irrigation water channel as the research object, irrigation water sediment char-acteristics was analyzed. The Delft3 D model was used to establish a two-dimensional channel sedi-ment transport mathematical model. Under the different channels of water level, flow velocity and flow sediment were studied. Calculation results show that:in Tahe irrigation area water channel fluc-tuation and flow rate of urgency with an average error of less than 0. 14m/s, peak flow error is less than 75m3/s;reasonable irrigation regime, sediment calculation error is less than 0. 02kg/m3;canal bed scouring and silting error 0. 023m, and measured values differed by 23%. The mathematical model can be to better reflect the irrigation ditch sediment sand flow and sediment transport.

  1. Outcome of Patients with Venous Thromboembolism and Factor V Leiden or Prothrombin 20210 Carrier Mutations During the Course of Anticoagulation.

    Science.gov (United States)

    Tzoran, Inna; Papadakis, Manolis; Brenner, Benjamin; Fidalgo, Ángeles; Rivas, Agustina; Wells, Philip S; Gavín, Olga; Adarraga, María Dolores; Moustafa, Farès; Monreal, Manuel

    2017-04-01

    Individuals with factor V Leiden or prothrombin G20210A mutations are at a higher risk to develop venous thromboembolism. However, the influence of these polymorphisms on patient outcome during anticoagulant therapy has not been consistently explored. We used the Registro Informatizado de Enfermedad TromboEmbólica database to compare rates of venous thromboembolism recurrence and bleeding events occurring during the anticoagulation course in factor V Leiden carriers, prothrombin mutation carriers, and noncarriers. Between March 2001 and December 2015, 10,139 patients underwent thrombophilia testing. Of these, 1384 were factor V Leiden carriers, 1115 were prothrombin mutation carriers, and 7640 were noncarriers. During the anticoagulation course, 160 patients developed recurrent deep vein thrombosis and 94 patients developed pulmonary embolism (16 died); 154 patients had major bleeding (10 died), and 291 patients had nonmajor bleeding. On multivariable analysis, factor V Leiden carriers had a similar rate of venous thromboembolism recurrence (adjusted hazard ratio [HR], 1.16; 95% confidence interval [CI], 0.82-1.64), half the rate of major bleeding (adjusted HR, 0.50; 95% CI, 0.25-0.99) and a nonsignificantly lower rate of nonmajor bleeding (adjusted HR, 0.66; 95% CI, 0.43-1.01) than noncarriers. Prothrombin mutation carriers and noncarriers had a comparable rate of venous thromboembolism recurrence (adjusted HR, 1.00; 95% CI, 0.68-1.48), major bleeding (adjusted HR, 0.75; 95% CI, 0.42-1.34), and nonmajor bleeding events (adjusted HR, 1.10; 95% CI, 0.77-1.57). During the anticoagulation course, factor V Leiden carriers had a similar risk for venous thromboembolism recurrence and half the risk for major bleeding compared with noncarriers. This finding may contribute to decision-making regarding anticoagulation duration in selected factor V Leiden carriers with venous thromboembolism. Copyright © 2017. Published by Elsevier Inc.

  2. Do Factor V Leiden and Prothrombin G20210A Mutations Predict Recurrent Venous Thromboembolism in Older Patients?

    Science.gov (United States)

    Méan, Marie; Limacher, Andreas; Stalder, Odile; Angelillo-Scherrer, Anne; Alberio, Lorenzo; Fontana, Pierre; Beer, Hans-Jürg; Rodondi, Nicolas; Lämmle, Bernhard; Aujesky, Drahomir

    2017-10-01

    The value of genetic thrombophilia testing in elderly patients with an unprovoked venous thromboembolism is unclear. We assessed whether the Factor V Leiden and the prothrombin G20210A mutation are associated with recurrent venous thromboembolism in elderly patients in a prospective multicenter cohort study. We genotyped the Factor V Leiden and the prothrombin G20210A mutation in 354 consecutive in- and outpatients aged ≥65 years with a first unprovoked venous thromboembolism from 9 Swiss hospitals. Patients and managing physicians were blinded to testing results. The outcome was recurrent symptomatic venous thromboembolism during follow-up. We examined the association between the Factor V Leiden and the prothrombin G20210A mutation and venous thromboembolism recurrence using competing risk regression, adjusting for age, sex, and periods of anticoagulation as a time-varying covariate. Overall, 9.0% of patients had a Factor V Leiden and 3.7% had a prothrombin G20210A mutation. At 36 months of follow-up, patients with a Factor V Leiden and a prothrombin G20210A mutation had a cumulative incidence of recurrent venous thromboembolism of 12.9% (95% confidence interval [CI], 5.1%-30.8%) and 18.5% (95% CI, 4.9%-56.5%), respectively, compared with 16.7% (95% CI, 12.5%-22.1%) of patients without mutation (P = .91 by the log-rank test). After adjustment, neither the Factor V Leiden (sub-hazard ratio 0.98; 95% CI, 0.35-2.77) nor the prothrombin G20210A mutation (sub-hazard ratio 1.15; 95% CI, 0.25-5.19) was associated with recurrent venous thromboembolism. Our results suggest that testing for genetic thrombophilia may not be beneficial in elderly patients with a first unprovoked venous thromboembolism. Copyright © 2017 Elsevier Inc. All rights reserved.

  3. HELLP Syndrome and Cerebral Venous Sinus Thrombosis Associated with Factor V Leiden Mutation during Pregnancy.

    Science.gov (United States)

    Dag, Zeynep Ozcan; Işik, Yuksel; Simsek, Yavuz; Tulmac, Ozlem Banu; Demiray, Demet

    2014-01-01

    Preeclampsia is a leading cause of maternal mortality and morbidity worldwide. The neurological complications of preeclampsia and eclampsia are responsible for a major proportion of the morbidity and mortality for women and their infants alike. Hormonal changes during pregnancy and the puerperium carry an increased risk of venous thromboembolism including cerebral venous sinus thrombosis (CVST). Factor 5 leiden (FVL) is a procoagulant mutation associated primarily with venous thrombosis and pregnancy complications. We report a patient with FVL mutation who presented with CVST at 24th week of pregnancy and was diagnosed as HELLP syndrome at 34th week of pregnancy.

  4. HELLP Syndrome and Cerebral Venous Sinus Thrombosis Associated with Factor V Leiden Mutation during Pregnancy

    Directory of Open Access Journals (Sweden)

    Zeynep Ozcan Dag

    2014-01-01

    Full Text Available Preeclampsia is a leading cause of maternal mortality and morbidity worldwide. The neurological complications of preeclampsia and eclampsia are responsible for a major proportion of the morbidity and mortality for women and their infants alike. Hormonal changes during pregnancy and the puerperium carry an increased risk of venous thromboembolism including cerebral venous sinus thrombosis (CVST. Factor 5 leiden (FVL is a procoagulant mutation associated primarily with venous thrombosis and pregnancy complications. We report a patient with FVL mutation who presented with CVST at 24th week of pregnancy and was diagnosed as HELLP syndrome at 34th week of pregnancy.

  5. Jewish Medical Students and Graduates at the Universities of Padua and Leiden: 1617–1740

    Directory of Open Access Journals (Sweden)

    Kenneth Collins

    2013-01-01

    Full Text Available The first Jewish medical graduates at the University of Padua qualified in the fifteenth century. Indeed, Padua was the only medical school in Europe for most of the medieval period where Jewish students could study freely. Though Jewish students came to Padua from many parts of Europe the main geographical sources of its Jewish students were the Venetian lands. However, the virtual Padua monopoly on Jewish medical education came to an end during the seventeenth century as the reputation of the Dutch medical school in Leiden grew. For aspiring medieval Jewish physicians Padua was, for around three hundred years, the first, simplest, and usually the only choice.

  6. The Leiden EXoplanet Instrument (LEXI): a high-contrast high-dispersion spectrograph

    Science.gov (United States)

    Haffert, S. Y.; Wilby, M. J.; Keller, C. U.; Snellen, I. A. G.

    2016-08-01

    The Leiden EXoplanet Instrument (LEXI) will be the first instrument designed for high-contrast, high-dispersion integral field spectroscopy at optical wavelengths. High-contrast imaging (HCI) and high-dispersion spectroscopy (HDS) techniques are used to reach contrasts of 10-7. LEXI will be a bench-mounted, high dispersion integral field spectrograph that will record spectra in a small area around the star with high spatial resolution and high dynamic range. A prototype is being setup to The Leiden EXoplanet Instrument (LEXI) will be the first instrument designed for high-contrast, high-dispersion integral field spectroscopy at optical wavelengths. High-contrast imaging (HCI) and high-dispersion spectroscopy (HDS) techniques are used to reach contrasts of 10-7. LEXI will be a bench-mounted, high dispersion integral field spectrograph that will record spectra in a small area around the star with high spatial resolution and high dynamic range. A prototype is being setup to test the combination of HCI+HDS and its first light is expected in 2016.

  7. Fibrinogen γ' increases the sensitivity to activated protein C in normal and factor V Leiden plasma.

    Science.gov (United States)

    Omarova, Farida; Uitte de Willige, Shirley; Simioni, Paolo; Ariëns, Robert A S; Bertina, Rogier M; Rosing, Jan; Castoldi, Elisabetta

    2014-08-28

    Activated protein C (APC) resistance, often associated with the factor V (FV) Leiden mutation, is the most common risk factor for venous thrombosis. We observed increased APC resistance in carriers of fibrinogen γ gene (FGG) haplotype 2, which is associated with reduced levels of the alternatively spliced fibrinogen γ' chain. This finding prompted us to study the effects of fibrinogen and its γ' chain on APC resistance. Fibrinogen, and particularly the γA/γ' isoform, improved the response of plasma to added APC in the thrombin generation-based assay. Similarly, a synthetic peptide mimicking the C-terminus of the fibrinogen γ' chain, which binds thrombin and inhibits its activities, greatly increased the APC sensitivity of normal and FV Leiden plasma, likely due to its ability to inhibit thrombin-mediated activation of FV and FVIII. Although the fibrinogen γ' peptide also inhibited protein C activation by the thrombin/thrombomodulin complex, it still increased the sensitivity of plasma to endogenously formed APC when thrombin generation was measured in the presence of soluble thrombomodulin. We conclude that fibrinogen, and particularly fibrinogen γ', increases plasma APC sensitivity. The fibrinogen γ' peptide might form the basis for pharmacologic interventions to counteract APC resistance. © 2014 by The American Society of Hematology.

  8. Frequency of Factor V Leiden and Prothrombin Polymorphism in South of Iran

    Directory of Open Access Journals (Sweden)

    Javad Dehbozorgian

    2009-06-01

    Full Text Available Normal hemostasis requires balanced regulation of prothromboticand antithrombotic factors. Inherited alteration of factor Vand prothrombin gene, the G20210A mutation, increases the resistanceof factor V to degradation and booster production ofprothrombin respectively. These alterations can increase hypercoagulabilityleading to thrombotic consequences. We aimed toassess the frequencies of these mutations in a group of the populationof southern Iran. In total, 198 healthy volunteers with theage range of 1-64 years were selected and screened for factor VLeiden and prothrombin mutations using polymerase chain reactionand restriction fragment length polymorphism techniques.The carrier frequencies for factor V Leiden and prothrombin mutationin the studied cohort were 4.1% and 3.07%, respectively.In the studied area, the allele frequency of factor V ishigher than the prothrombin G20210A mutation (0.0204 v0.0153. According to the data and Hardy-Weinberger equation,the total risk of thrombosis caused by homozygosity andheterozygosity of factor V Leiden, prothrombin G20210A mutationand compound heterozygosity of these mutations areabout 1 in 500 individuals.

  9. Multiple Brain Abscesses in an Immunocompetent Patient With Factor V Leiden Mutation.

    Science.gov (United States)

    Zafar, Saeed Zubair; Pervin, Najwa; Manthri, Sukesh; Bhattarai, Mukul

    2016-01-01

    Multiple brain abscesses in an immunocompetent patient is a challenging clinical problem in the medical world despite advances in imaging techniques, laboratory diagnostics, surgical interventions, and antimicrobial treatment. It is a clinical entity that typically tends to occur in the presence of known predisposing factors. Clinicians seek to determine the potential risk factors responsible for the development of brain abscess because it is very crucial for management of this life-threatening condition. At times, like in our case, there are clinical situations where it is difficult to reveal any traditional risk factors. We report a case of multiple brain abscesses in a 51-year-old female with a past medical history significant only for factor V Leiden mutation, and deep vein thrombosis on chronic anticoagulation. She underwent thorough evaluation but no predisposing factors were found. Based on our extensive literature review, this is the index case of multiple brain abscesses in a patient with history of factor V Leiden mutation and the absence of any conventional risk factors. We also postulate a possible mechanism of infection in such patients.

  10. Life threatening pulmonary embolus in a factor V Leiden carrier on oral contraceptives: a case report.

    Science.gov (United States)

    Saif, M W; Volpe, B T; Dailey, M; Tsongalis, G J

    1997-06-01

    Venous thromboembolism is a serious, potentially lethal health problem affecting one per 1,000 people annually. Major surgery, the use of oral contraceptives, complicated pregnancy, fractures, and immobilization increase the risk of thrombosis. In addition to these factors, thrombosis is associated with inherited deficiencies of antithrombin III, protein C, and protein S. Together these do not account for more than five to 10% of the cases. Hereditary activated protein C resistance has been recognized as a basis for a majority of cases of familial thrombosis. It accounted for more than a 10 times higher number than that of other known genetic defects. We describe a case of a young female who presented with a pulmonary embolism and was discovered to have activated protein C resistance. This patient had a heterozygous mutation for factor V Leiden and was taking oral contraceptives. This report underlines: 1) increased risk of venous thrombosis in oral contraceptive users who carry factor V Leiden mutation associated with functional resistance to the normal anticoagulation activities of protein C; 2) most episodes occurring in the young are minor, but pulmonary embolus can occur; 3) the importance of identifying other affected members of the family; and 4) the importance of anticoagulation prophylaxis at times of enhanced risk, particularly during pregnancy, postpartum, and major surgery.

  11. Factor V G1691A (Leiden is a major etiological factor in Egyptian Budd-Chiari syndrome patients

    Directory of Open Access Journals (Sweden)

    Tawhida Y. Abdel Ghaffar

    2011-12-01

    Full Text Available Objective: Budd-Chiari syndrome is a multifactorial disease in which several prothrombotic disorders may predispose patients to the development of thrombosis at this uncommon location (hepatic veins. The aim of this study was to determine the prevalence and characteristics of inherited thrombophilia in Egyptian Budd-Chiari syndrome patients.Materials and Methods: The study included 47 Budd-Chiari syndrome patients (20 children and 27 adults. Genotyping of Factor V G1691A (Leiden, prothrombin G20210A (PT, and methylenetetrahydrofolate reductase C677T were performed using real-time PCR and fluorescence melting curve detection analysis.Results: Factor V Leiden was observed in 29 patients (61.7%. It is the only factor that caused Budd-Chiari syndrome in 18 of the patients and in 5 of the patients with inferior vena cava involvement. Myeloproliferative disease was noted in 12 (25.5% patients, antiphospholipid syndrome in 5 (10.6%, and Behcet’s disease in 3 (6.4%. Interestingly, 3 of the children with Budd-Chiari syndrome had lipid storage disease.Conclusion: Factor V Leiden was a major etiological factor in Egyptian Budd-Chiari syndrome patients, which may have been related to the high frequency of this mutation in the study region. Factor V Leiden was also a strong thrombophilic factor and the leading cause of inferior vena cava thrombosis in these patients. Lipid storage disease should be included as a risk factor for Budd-Chiari syndrome.

  12. Catalogue of the Mesozoic and Cenozoic holotypes in the collection of plant fossils in the Nationaal Natuurhistorisch Museum, Leiden

    NARCIS (Netherlands)

    Konijnenburg-van Cittert, van J.H.A.; Waveren, van I.M.; Jonckers, J.B.

    2004-01-01

    This is an inventory of the Mesozoic and Cenozoic type material in the original palaeobotanical collections of the Nationaal Natuurhistorisch Museum, Leiden, The Netherlands. In total 60 holotypes are documented and one is noted as missing from the collections. One new combination is made (Cinnamomu

  13. Cenozoic Molluscan types from Java (Indonesia) in the Martin Collection (Division of Cenozoic Mollusca), National Museum of Natural History, Leiden

    NARCIS (Netherlands)

    Hoek Ostende, van den L.W.; Leloux, J.; Wesselingh, F.P.; Winkler Prins, C.F.

    2002-01-01

    An inventory of type material in the ‘Martin Collection’ at the Division of Cenozoic Mollusca of the National Museum of Natural History, Leiden, The Netherlands has been made. In total 1842 lots containing over 5700 type specimens of 912 species were encountered. The status of the types is outlined.

  14. Has one of Captain Cook’s possums landed in Leiden? The possible holotype of Pseudocheirus peregrinus (Boddaert, 1785)

    NARCIS (Netherlands)

    Smeenk, C.

    2009-01-01

    The identity of an old female specimen of Pseudocheirus peregrinus (Boddaert, 1785) in the National Museum of Natural History, Leiden, is discussed and the early descriptions and nomenclatural history of the species are reviewed. The assumption by Temminck (1824) and Jentink (1888) that the animal o

  15. Basement evolution in the Northern Hesperian Massif. A preliminary survey of results obtained by the Leiden research group

    NARCIS (Netherlands)

    Tex, den E.

    1981-01-01

    Historical notes on Galician geology, and on the work of the Leiden University petrology team in particular, are first provided. This is followed by an introduction to the geology of Galicia with emphasis on its crystalline basement and upper mantle inliers. Six lithotectonic units are

  16. Relative validity of the food frequency questionnaire used to assess dietary intake in the Leiden Longevity Study

    NARCIS (Netherlands)

    Streppel, M.T.; Vries, de J.H.M.; Meyboom, S.; Beekman, M.; Craen, A.J.M.; Slagboom, P.E.; Feskens, E.J.M.

    2013-01-01

    Background - Invalid information on dietary intake may lead to false diet-disease associations. This study was conducted to examine the relative validity of the food frequency questionnaire (FFQ) used to assess dietary intake in the Leiden Longevity Study. Methods - A total of 128 men and women part

  17. Effects of dietary fish oil on serum lipids and VLDL kinetics in hyperlipidemic apolipoprotein E*3-Leiden transgenic mice

    NARCIS (Netherlands)

    Vlijmen, B.J.M. van; Mensink, R.P.; Hof, H.B. van 't; Offermans, R.F.G.; Hofker, M.H.; Havekes, L.M.

    1998-01-01

    Studying the effects of dietary fish oil on VLDL metabolism in humans is subject to both large intra- and interindividual variability. In the present study we therefore used hyperlipidentic apolipoprotein (APO) E*3-Leiden mice, which have impaired chylomicron and very low density lipoprotein (VDL) r

  18. Risk of venous thromboembolism in carriers of factor V Leiden with a concomitant inherited thrombophilic defect : a retrospective analysis

    NARCIS (Netherlands)

    Meinardi, [No Value; Middeldorp, S; de Kam, PJ; Koopman, AMW; van Pampus, ECM; Hamulyak, K; Prins, MH; Buller, HR; van der Meer, J

    2001-01-01

    Factor V Leiden is the most common genetic defect associated with venous thromboembolism. Its clinical expression is limited and shows a wide intrafamilial and interfamilial variation, which might be explained by the influence of other genetic risk factors. We retrospectively studied 226 patients

  19. The risk of pregnancy-related venous thromboembolism in women who are homozygous for factor V Leiden

    NARCIS (Netherlands)

    Middeldorp, S; Libourel, EJ; Hamulyak, K; van der Meer, J; Buller, HR

    The risk of venous thromboembolism (VTE) is increased in pregnancy and during the post-partum period, The absolute risk for pregnancy-related VTE in heterozygous women with the factor V Leiden mutation is approximately 2%, but studies on this risk for homozygous women show conflicting results. In a

  20. Life-threatening aortic thrombosis in a trauma patient homozygous for factor V Leiden mutation: Case report

    Directory of Open Access Journals (Sweden)

    Kopterides Petros

    2011-05-01

    Full Text Available Abstract We report a case of near fatal aortic thrombosis in a trauma patient homozygous for mutation of Factor V Leiden. He responded well to vascular surgery and intensive care unit management and was discharged successfully from the hospital one month later.

  1. Impact of the factor V Leiden mutation on the outcome of pneumococcal pneumonia: a controlled laboratory study

    NARCIS (Netherlands)

    Schouten, M.; van 't Veer, C.; Roelofs, J.J.; Levi, M.; van der Poll, T.

    2010-01-01

    Introduction: Streptococcus (S.) pneumoniae is the most common cause of community-acquired pneumonia. The factor V Leiden (FVL) mutation results in resistance of activated FV to inactivation by activated protein C and thereby in a prothrombotic phenotype. Human heterozygous FVL carriers have been re

  2. Rosuvastatin reduces plasma lipids by inhibiting VLDL production and enhancing hepatobiliary lipid excretion in ApoE*3-Leiden mice

    NARCIS (Netherlands)

    Delsing, DJM; Post, SM; Groenendijk, M; Solaas, K; van der Boom, H; van Duyvenvoorde, W; de Wit, ECM; Bloks, VW; Kuipers, F; Havekes, LM; Princen, HMG

    The present study was designed to investigate the lipid-lowering properties and mechanisms of action of a new HMG-CoA reductase inhibitor, rosuvastatin, in female ApoE*3-Leiden transgenic mice. Mice received a high fat/cholesterol (HFC) diet containing either rosuvastatin (0 [control], 0.00125%,

  3. Charles Darwin’s lost Cinereous Harrier found in the collection of the National Museum of Natural History Leiden

    NARCIS (Netherlands)

    Grouw, van H.; Steinheimer, F.D.

    2008-01-01

    During reorganisation of the Leiden bird collection a mounted harrier was found what seemed to be one of the still lost specimens collected by Darwin. After closer research it turned out it was indeed the last missing harrier of the Darwin collection.

  4. Cholesteryl ester transfer protein decreases high-density lipoprotein and severely aggravates atherosclerosis in APOE*3-Leiden mice

    NARCIS (Netherlands)

    M. Westerterp (Marit); C.C. van der Hoogt (Caroline); W. de Haan (Willeke); E. Offerman (Erik); G.M. Dallinga-Thie (Geesje); J.W. Jukema (Jan Wouter); L.M. Havekes (Louis); P.C.N. Rensen (Patrick)

    2006-01-01

    textabstractOBJECTIVE - The role of cholesteryl ester transfer protein (CETP) in the development of atherosclerosis is still undergoing debate. Therefore, we evaluated the effect of human CETP expression on atherosclerosis in APOE*3-Leiden (E3L) mice with a humanized lipoprotein profile. METHODS AND

  5. A reidentification of the Australian Muridae in the Leiden Museum listed by F. A. Jentink in 1887 and 1888

    NARCIS (Netherlands)

    Mahoney, J.A.

    1969-01-01

    A number of Australian rodent specimens are listed by Jentink (1887; 1888) in two catalogues of the mammals in the Rijksmuseum van Natuurlijke Historie, Leiden, Netherlands. The 1887 catalogue deals with the osteological material; in it, thirteen Australian rodent skulls are recorded. The 1888 catal

  6. Charles Darwin’s lost Cinereous Harrier found in the collection of the National Museum of Natural History Leiden

    NARCIS (Netherlands)

    Grouw, van H.; Steinheimer, F.D.

    2008-01-01

    During reorganisation of the Leiden bird collection a mounted harrier was found what seemed to be one of the still lost specimens collected by Darwin. After closer research it turned out it was indeed the last missing harrier of the Darwin collection.

  7. Factor V Leiden mutation increases the risk for venous thromboembolism in cancer patients - results from the Vienna Cancer And Thrombosis Study (CATS).

    Science.gov (United States)

    Pabinger, I; Ay, C; Dunkler, D; Thaler, J; Reitter, E-M; Marosi, C; Zielinski, C; Mannhalter, C

    2015-01-01

    Patients with cancer are at an increased risk for venous thromboembolism (VTE). The risk varies markedly in different patient populations. Factor V (FV) Leiden is the most common genetic risk factor for VTE, and the impact of FV Leiden on cancer-associated thrombosis is not yet fully elucidated. To study the impact of FV Leiden on the risk of thrombosis in cancer patients. In the prospective observational Vienna Cancer And Thrombosis Study (CATS), 982 patients were included and were followed until occurrence of VTE or death, for a maximum period of 2 years. FV Leiden was determined by genotyping at inclusion. Main outcome measures were symptomatic or lethal objectively confirmed VTE. Of the 982 patients, FV Leiden was diagnosed in 72 (7.3%, 70 were heterozygous and 2 were homozygous). Ten of 72 (13.9%) patients with FV Leiden developed VTE, whereas this was the case in 69 of 910 (7.6%) patients without FV Leiden. In multivariate analysis that included age, sex, different tumor types, tumor stage, newly diagnosed vs. recurrence of disease, and the treatment modalities, the hazard ratio was 2.0 (95% confidence interval 1.0-4.0). In Kaplan-Meier analysis, the probability for development of VTE was 13% in those with and 5.7% in those without FV Leiden after 6 months; after 1 year, the corresponding risks were 15% and 7.3%. FV Leiden is a genetically determined and thus disease-independent parameter, which is associated with VTE in cancer patients and could therefore be used for individual risk assignment. © 2014 International Society on Thrombosis and Haemostasis.

  8. Thrombomodulin-dependent effect of factor V Leiden mutation on the cross-linking of α2-plasmin inhibitor to fibrin and its consequences on fibrinolysis.

    Science.gov (United States)

    Koncz, Zsuzsa; Bagoly, Zsuzsa; Haramura, Gizella; Mezei, Zoltán A; Muszbek, László

    2012-09-01

    It has been shown that thrombomodulin (TM) considerably delays factor XIII (FXIII) activation and this effect is abrogated by Factor V Leiden (FV(Leiden)) mutation. The aim of the study was to explore the effect of TM on the cross-linking of α(2)-plasmin inhibitor (α(2)-PI) to fibrin in plasma samples of different FV genotypes and how this effect is related to the impaired fibrinolysis of FV(Leiden) carriers. In the plasma samples of fifteen individuals with different FV genotypes and in FV deficient plasma supplemented with wild type FV or FV(Leiden) coagulation was initiated by recombinant human tissue factor and phospholipids with or without recombinant human TM (rhTM). In the recovered clots the extent of α(2)-PI-fibrin cross-linking was evaluated by Western blotting and quantitative densitometry. The effect of rhTM on tissue plasminogen activator (tPA) induced clot lysis was measured by turbidimetric method. rhTM significantly delayed the formation of α(2)-PI-fibrin α-chain heterodimers/oligomers in plasma samples containing wild type FV. This effect of rhTM was impaired in the presence of FV(Leiden). rhTM delayed tPA-induced clot lysis and this effect of rhTM was more pronounced in plasma containing FV(Leiden). When TAFIa was inhibited by potato carboxypeptidase inhibitor, rhTM accelerated clot lysis in the presence of wild type FV, which is explained by the delayed α(2)-PI-fibrin cross-linking. This effect of rhTM did not prevail in the presence of FV(Leiden). FV(Leiden) abrogates the delaying effect of rhTM on α(2)-PI-fibrin cross-linking, which contributes to the impaired fibrinolysis observed in FV(Leiden) carriers. Copyright © 2012 Elsevier Ltd. All rights reserved.

  9. Meta-analysis of factor V Leiden and ischemic stroke in young adults: the importance of case ascertainment.

    Science.gov (United States)

    Hamedani, Ali G; Cole, John W; Mitchell, Braxton D; Kittner, Steven J

    2010-08-01

    The factor V Leiden mutation is associated with ischemic stroke in children but not in adults. Whether it is associated with ischemic stroke in young adults, however, is uncertain. To address this issue, we performed a meta-analysis of 18 case-control studies of ischemic stroke in adults 50 years of age and younger published before June 2009. Across all studies, factor V Leiden was detected in 154 of 2045 cases (7.5%) and 217 of 5307 controls (4.1%), yielding a fixed-effect odds ratio of 2.00 (95% CI, 1.59-2.51). However, further analyses revealed substantial heterogeneity among these studies (P=0.005 for Q-test of heterogeneity). Hypothesizing that this heterogeneity could be related to differences among studies in case selection criteria, we stratified the meta-analysis into studies for which case samples were enriched or not enriched to include cases having an increased likelihood of prothrombotic genetic involvement ("selected" ischemic stroke studies, n=9) and those that recruited cases from consecutive neurology referrals or hospitalizations ("unselected" ischemic stroke studies, n=8). Among the 9 "selected" ischemic stroke studies, factor V Leiden was more strongly associated with stroke (OR, 2.73; 95% CI, 1.98-3.75), whereas among the 8 "unselected" ischemic stroke studies, the association between factor V Leiden and stroke was substantially weaker (OR, 1.40; 95% CI, 0.998-1.95). This difference was found to be statistically significant (P=0.003 for Woolf test for heterogeneity). We conclude that factor V Leiden is associated with ischemic stroke in young adults, particularly in patient populations in which there is an increased clinical suspicion of prothrombotic state.

  10. Rastreamento familiar do fator V de Leiden: a importância da detecção de portadores heterozigotos Familiar tracking of factor V Leiden: the importance of detection in heterozygous carriers

    Directory of Open Access Journals (Sweden)

    Eunice B. Carvalho

    2005-06-01

    Full Text Available O fator de Leiden é uma mutação genética que predispõe seus portadores ao tromboembolismo venoso. O objetivo do estudo foi investigar a distribuição dos alelos em 21 membros da família de três pacientes portadores de trombose com a presença da mutação do fator V de Leiden. A detecção da mutação no gene do fator V foi realizada entre portadores da mutação no estado heterozigoto. Este estudo foi realizado no Centro de Hematologia e Hemoterapia do Ceará - Hemoce. Observou-se a presença da mutação no estado heterozigoto na família 1 (83,3%, na família 2 (40% e na família 3 (50%. No total de 24 membros (pacientes e familiares analisados, 50% (12/24 apresentaram a mutação, todos no estado heterozigoto, 66,7% (8/12 não apresentaram trombose. A detecção do fator V de Leiden em pacientes portadores de eventos trombóticos é recomendado para esclarecimento das causas e para efetuar o rastreamento em membros de sua família, ainda sem o aparecimento de eventos trombóticos, de forma a avaliar os riscos associados e assim determinar um acompanhamento médico preventivo.Factor V Leiden is a mutation that can cause venous thrombosis. When associated to other risk factors such as the use of contraceptives, important surgical intervention, pregnancy and malignant diseases, the risk for heterozygous carriers increases by three-fold to 10 times or even 80 times for two mutated alleles. The factor V Leiden is found in about 20% of the population with a history of venous thromboembolism. It is present in about 4 to 6% of general population but this percentage changes depending on the ethnicity. This study shows the distribution of alleles in family members of three carriers of factor V Leiden diagnosed with deep venous thrombosis. The mutation investigation of the factor V Leiden gene was performed in 21 family members of 3 heterozygous carriers. The study was performed in the Hematology and Hemotherapy Center from Ceará - Hemoce

  11. Delft3D Storm Surge Simulation of Typhoon Haiyan for Projection of Coastal Inundation in the Visayas Islands, Philippines

    Science.gov (United States)

    Suarez, John Kenneth; Cabacaba, Krichi May; Biton, Nophi Ian; Cuadra, Camille; Santiago, Joy; Mendoza, Jerico; Mahar Francisco Lagmay, Alfredo

    2015-04-01

    The Philippines is geographically prone to tropical cyclones with an annual average of 20 typhoons entering the country's area of responsibility. Majority of these typhoons pass through the central part of the archipelago in the Visayas Region. On 08 November 2013, a Category 5 Typhoon Haiyan with maximum ten-minute sustained wind speed of 230 kph hit the Visayas region causing damage amounting to two billion US dollars with 6,300 reported casualties. The adverse impacts of future storm surge events in the Philippine archipelago, specifically in the Visayan region, can be mitigated if a storm surge model that will include the inundation of coastal areas is generated. The hydrodynamic modeling software, Delft3D, was used in creating hydrodynamic models for areas in the Visayas Islands. High resolution hydrodynamic models of the hardly stricken areas with 10-m per pixel resolution Digital Elevation Model and General Bathymetric Chart of the Oceans bathymetry were nested to an overall model of Visayas with a coarse grid cell size. Due to the lack of observed water level data during the onslaught of Typhoon Haiyan, the overall Visayas model was calibrated by creating hydrodynamic models for the same Haiyan-affected areas using previous typhoons with recorded data acquired from tide stations as wind forcing. Several simulations were carried out to generate the farthest possible inland incursion of storm surges. This was done by translating the actual typhoon track vertically and horizontally with a specified increment. The output of the study is a storm surge inundation map showing the worst case scenario of inundation for a Category 5 typhoon. This storm surge inundation map can be used to determine safe zones for development of infrastructure near coastal areas. The storm surge inundation map can also be used as basis for disaster preparedness plans of coastal communities threatened by approaching typhoons.

  12. Die Leiden des jungen Werthers à luz da história do conceito de subjetividade

    Directory of Open Access Journals (Sweden)

    Felipe Vale da Silva

    2013-06-01

    Full Text Available O artigo analisa os paralelos entre o retrato do 'sujeito moderno em crise' visto no romance Die Leiden des jungen Werthers de Goethe e, por outro lado, o perfil psicossocial do 'homem do sentimento' do século XVIII, fruto da cultura da Empfindsamkeit. Defendo a perspectiva de que, no romance, Goethe não apenas se utiliza do formato literário mais tradicional da Empfindsamkeit (o do romance epistolar, como também se apropria de topói e técnicas discursivas que lhe são próprias em registro radicalmente heterodoxo. A argumentação nos ligará a uma dedução da visão do conceito de subjetividade moderna com que Goethe trabalha em sua fase final do Sturm und Drang, e que o situa como importante nome do discurso filosófico da modernidade.

  13. A male adolescent with left iliac thrombophlebitis and heterozygosity for factor V Leiden mutation.

    Science.gov (United States)

    Lovejoy, Amy E; Bush, Ruth A; Pong, Alice L; Hilfiker, Mary L

    2009-08-01

    The epidemiology of deep vein thrombosis in adolescents has 2 potential associations. First, there is a demonstrated association with a congenital anomaly of the inferior vena cava (Dean SM, Tytle TL. Vas Med. 2006;11:165-169; Schnieider JG, Eynatten MV, Dugi KA, et al. J Intern Med. 2002;252:276-280). Secondly, resistance to activated protein C as a result of factor V Leiden is associated with thromboembolic disease at an early age (Price DT, Ridker PM. Ann Intern Med. 1997;127:895-903). Imaging modalities, central venous catheters, and improved life expectancy for critically and chronically ill children have resulted in an increased diagnosis of thromboembolic disease in the pediatric population (Journeycake MM, Manco-Johnson MJ. Hematol Oncol Clin N Am. 2004;18;1315-1338), and evaluation for thrombophilia should be performed for any child with thromboembolic disease.

  14. EPIDEMIOLOGY OF ACTIVATED PROTEIN C RESISTANCE AND FACTOR V LEIDEN MUTATION IN THE MEDITERRANEAN REGION

    Directory of Open Access Journals (Sweden)

    Mehrez Mehrez M. Jadaon

    2011-09-01

    Full Text Available Venous thromboembolic disorders (VTE are serious disorders with high morbidity and mortality rates. Many genetic and acquired risk factors were identified to cause VTE The most common genetic risk factor is Factor V Leiden mutation (FVL. FVL was found in high percentage of populations of Caucasian origin but was almost absent in non-Caucasians. It was also reported in populations living in North Africa and the Middle East.  This review article briefly explains FVL and how it causes VTE, the distribution of FVL worldwide, and then it elaborates on the epidemiology of FVL in the Mediterranean Region and how this brought speculations that FVL might have originated in the Eastern Mediterranean area.

  15. What We Have Learned from San Francisco Declaration on Research Assessment and Leiden Manifesto?

    Directory of Open Access Journals (Sweden)

    Carey Ming-Li Chen

    2017-03-01

    Full Text Available In recent years, the research performance evaluation of members of the academic community conducted by government or institutions has been applied with multiple indicators and peer review, however, there are many controversies about the design and application of research evaluation indicators. This article aims to introduce the development process of San Francisco Declaration on Research Assessment (DORA and Leiden Manifesto and summarize their contents of guidelines and attempts to compare the differences between these two documents. It hopes that this article can arise the attention and reflection of research evaluation indicators and relevant issues from Taiwan academic community to reach consensus of utilization of research evaluation indicators. It will be beneficial to develop the version of declaration with local characteristics in the future.

  16. Epidemiology of Activated Protein C Resistance and Factor V Leiden Mutation in the Mediterranean Region

    Science.gov (United States)

    Jadaon, Mehrez M.

    2011-01-01

    Venous thromboembolic disorders (VTE) are serious disorders with high morbidity and mortality rates. Many genetic and acquired risk factors were identified to cause VTE. The most common genetic risk factor is Factor V Leiden mutation (FVL). FVL was found in high percentage of populations of Caucasian origin but was almost absent in non-Caucasians. It was also reported in populations living in North Africa and the Middle East. This review article briefly explains FVL and how it causes VTE, the distribution of FVL worldwide, and then it elaborates on the epidemiology of FVL in the Mediterranean Region and how this brought speculations that FVL might have originated in the Eastern Mediterranean area. PMID:22224194

  17. A Young Male Patient With Multiple Thromboembolisms Associated With Factor V Leiden Mutation.

    Science.gov (United States)

    Çinier, Göksel; Öz, Ahmet; Tekkesin, Ahmet Ilker; Hayıroğlu, Mert İlker; Keskin, Muhammed; Avsar, Şahin

    2016-09-28

    Factor V Leiden (FVL) mutation is the most common hereditary thrombophilia. Association of this mutation with venous thrombosis is well established. However, there are several conflicting results regarding the association of FVL with arterial thrombosis, acute coronary syndrome, and intracardiac thrombosis. In this case report, we present a 44-yearold male patient with a medical history of both arterial and venous thrombosis who came to our emergency department with chest pain. After the initial evaluation he was diagnosed as having acute coronary syndrome and transthoracic echocardiography revealed an intracardiac apical thrombus. Coronary angiography showed non-critical stenosis. Thrombophilia panel was studied and the patient was found to be heterozygotic for FVL mutation. An apical thrombus was extracted surgically because of the high risk of systemic embolization.

  18. An improved algorithm for activated protein C resistance and factor V Leiden screening.

    Science.gov (United States)

    Herskovits, Adrianna Z; Morgan, Elizabeth A; Lemire, Susan J; Lindeman, Neal I; Dorfman, David M

    2013-09-01

    To evaluate the performance of a Russell viper venom-based activated protein C resistance (APCR) screening test relative to DNA analysis for the factor V Leiden mutation. We evaluated the concordance between Pefakit APCR screening results and DNA analysis for 435 patients homozygous (n = 11), heterozygous (n = 310), or wild-type (n =114) for the G1691A allele. Using receiver operating characteristic analysis, we found that a cutoff of 1.89 for the APCR ratio yields a sensitivity and specificity of 99.1%. In patients with discrepant genotype-phenotype correlation, their APCR may provide a more clinically relevant result. We compared several strategies for employing reflex testing and found that performing initial APCR screening followed by confirmatory molecular analysis on a subset of cases in the borderline regions between the diagnostic groups can reduce unnecessary testing by approximately 80% without compromising diagnostic accuracy.

  19. Recurrent pregnancy loss in a subject with heterozygote factor V Leiden mutation; a case report

    Science.gov (United States)

    Ebrahimzadeh-Vesal, Reza; Azam, Roza; Ghazarian, Arvin; Hajesmaeili, Mogge; Ranji, Najmeh; Ezzati, Mohammad Reza; Sadri, Mehrdad; Mohammadi, Mohammad Ali; Khavandi, Siamak

    2014-01-01

    Recurrent pregnancy loss is usually defined as the loss of two or more consecutive pregnancies before 20 weeks of gestation, which occurs in approximately 5% of reproductive-aged women. It has been suggested that women with thrombophilia have an increased risk of pregnancy loss and other adverse pregnancy outcomes. Thrombophilia is an important predisposition to blood clot formation and is considered as a significant risk factor for recurrent pregnancy loss. The inherited predisposition to thrombophilia is most often associated with factor V Leiden mutation, prothrombin G20210A mutation, and methylenetetrahydrofolate reductase C677T and A1298C gene variants. The net effect is an increased cleavage of prothrombin to thrombin and excessive blood coagulation. PMID:26989729

  20. Increased activation of blood coagulation in pregnant women with the Factor V Leiden mutation.

    Science.gov (United States)

    Kjellberg, Ulla; van Rooijen, Marianne; Bremme, Katarina; Hellgren, Margareta

    2014-10-01

    The risk of venous thromboembolism is enhanced in pregnant carriers of the Factor V Leiden mutation. The primary aim of the study was to compare prothrombin fragments 1+2, soluble fibrin and D-dimer levels in pregnant Factor V Leiden mutation carriers with those in non-carriers. Secondary aims were to evaluate whether these biomarkers could predict placenta-mediated complications or venous thromboembolism, and to study blood coagulation after caesarean section with thromboprophylaxis and after vaginal delivery without thromboprophylaxis. Prothrombin fragments 1+2, soluble fibrin and D-dimer levels were studied longitudinally in 476 carriers with singleton pregnancies from gestational weeks 23-25 until 8-10 weeks postpartum. Prothrombin fragments 1+2 and D-dimer levels gradually increased during pregnancy. D-dimer levels were higher in carriers, both during pregnancy and puerperium, compared to non-carriers. D-dimer levels above 0.5mg/l were found in about 30% and 20% of the heterozygous carriers at 4-5 and 8-10 weeks postpartum, respectively. Soluble fibrin levels were mainly unchanged during pregnancy, with no difference between carriers and non-carriers. Biomarker levels were similar in carriers with uncomplicated and complicated pregnancies. Higher D-dimer levels indicate increased blood coagulation and fibrinolysis activity in carriers. The high proportion of carriers with D-dimer levels exceeding 0.5mg/l postpartum must be considered when assessing the probability of venous thromboembolism. Large overlaps in biomarker levels in normal and complicated pregnancies suggest that these biomarkers cannot be used as predictors. Thromboprophylaxis following caesarean section may prevent increased activation of blood coagulation. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. Sensitized mutagenesis screen in Factor V Leiden mice identifies thrombosis suppressor loci.

    Science.gov (United States)

    Westrick, Randal J; Tomberg, Kärt; Siebert, Amy E; Zhu, Guojing; Winn, Mary E; Dobies, Sarah L; Manning, Sara L; Brake, Marisa A; Cleuren, Audrey C; Hobbs, Linzi M; Mishack, Lena M; Johnston, Alexander J; Kotnik, Emilee; Siemieniak, David R; Xu, Jishu; Li, Jun Z; Saunders, Thomas L; Ginsburg, David

    2017-09-05

    Factor V Leiden (F5(L) ) is a common genetic risk factor for venous thromboembolism in humans. We conducted a sensitized N-ethyl-N-nitrosourea (ENU) mutagenesis screen for dominant thrombosuppressor genes based on perinatal lethal thrombosis in mice homozygous for F5(L) (F5(L/L) ) and haploinsufficient for tissue factor pathway inhibitor (Tfpi(+/-) ). F8 deficiency enhanced the survival of F5(L/L)Tfpi(+/-) mice, demonstrating that F5(L/L)Tfpi(+/-) lethality is genetically suppressible. ENU-mutagenized F5(L/L) males and F5(L/+)Tfpi(+/-) females were crossed to generate 6,729 progeny, with 98 F5(L/L)Tfpi(+/-) offspring surviving until weaning. Sixteen lines, referred to as "modifier of Factor 5 Leiden (MF5L1-16)," exhibited transmission of a putative thrombosuppressor to subsequent generations. Linkage analysis in MF5L6 identified a chromosome 3 locus containing the tissue factor gene (F3). Although no ENU-induced F3 mutation was identified, haploinsufficiency for F3 (F3(+/-) ) suppressed F5(L/L)Tfpi(+/-) lethality. Whole-exome sequencing in MF5L12 identified an Actr2 gene point mutation (p.R258G) as the sole candidate. Inheritance of this variant is associated with suppression of F5(L/L)Tfpi(+/-) lethality (P = 1.7 × 10(-6)), suggesting that Actr2(p.R258G) is thrombosuppressive. CRISPR/Cas9 experiments to generate an independent Actr2 knockin/knockout demonstrated that Actr2 haploinsufficiency is lethal, supporting a hypomorphic or gain-of-function mechanism of action for Actr2(p.R258G) Our findings identify F8 and the Tfpi/F3 axis as key regulators in determining thrombosis balance in the setting of F5(L) and also suggest a role for Actr2 in this process.

  2. Risk of venous thromboembolism and myocardial infarction associated with factor V Leiden and prothrombin mutations and blood type.

    Science.gov (United States)

    Sode, Birgitte F; Allin, Kristine H; Dahl, Morten; Gyntelberg, Finn; Nordestgaard, Børge G

    2013-03-19

    ABO blood type locus has been reported to be an important genetic determinant of venous and arterial thrombosis in genome-wide association studies. We tested the hypothesis that ABO blood type alone and in combination with mutations in factor V Leiden R506Q and prothrombin G20210A is associated with the risk of venous thromboembolism and myocardial infarction in the general population. We used data from 2 Danish studies that followed members of the general public from 1977 through 2010. We obtained the genotype of 66 001 white participants for ABO blood type, factor V Leiden R506Q and prothrombin G20210A. We calculated hazard ratios (HRs) and population attributable risk. Our main outcome measures were venous thromboembolism and myocardial infarction. The multivariable adjusted HR for venous thromboembolism was 1.4 (95% confidence interval [CI] 1.3-1.5) for non-O blood type (v. O blood type). For the factor V Leiden R506Q mutation, the adjusted HR was 2.2 (95% CI 2.0-2.5) for heterozygous participants and 7.0 (95%CI 4.8-10) for homozygous participants (v. participants without the mutation). For prothrombin G20210A, the adjusted HR was 1.5 (95%CI 1.2-1.9) for heterozygous participants and 11 (95% CI 2.8-44) for homozygous participants (v. participants without the mutation). When we combined ABO blood type and factor V Leiden R506Q or prothrombin G20210A genotype, there was a stepwise increase in the risk of venous thromboembolism (trend, pfactor V Leiden R506Q and 1% for prothrombin G20210A. Multivariable adjusted HRs for myocardial infarction by genotypes did not differ from 1.0. ABO blood type had an additive effect on the risk of venous thromboembolism when combined with factor V Leiden R506Q and prothrombin G20210A mutations; blood type was the most important risk factor for venous thromboembolism in the general population.

  3. Risk of recurrent venous thrombosis in homozygous carriers and double heterozygous carriers of factor V Leiden and prothrombin G20210A.

    Science.gov (United States)

    Lijfering, Willem M; Middeldorp, Saskia; Veeger, Nic J G M; Hamulyák, Karly; Prins, Martin H; Büller, Harry R; van der Meer, Jan

    2010-04-20

    Homozygous or double heterozygous factor V Leiden and/or prothrombin G20210A is a rare inherited thrombophilic trait. Whether individuals with this genetic background have an increased risk of recurrent venous thrombosis is uncertain. A case-control design within a large cohort of families with thrombophilia was chosen to calculate the risk of recurrent venous thrombosis in individuals with homozygosity or double heterozygosity of factor V Leiden and/or prothrombin G20210A. Cases were individuals with recurrent venous thrombosis, and controls were those with only 1 venous thrombosis. The cohort consisted of 788 individuals with venous thrombosis; 357 had factor V Leiden, 137 had prothrombin G20210A, 27 had factor V Leiden and/or prothrombin G20210A homozygosity, and 49 had double heterozygosity for both mutations. We identified 325 cases with recurrent venous thrombosis and 463 controls with only 1 venous thrombosis. Compared with noncarriers, crude odds ratio for recurrence was 1.2 (95% confidence interval, 0.9 to 1.6) for heterozygous carriers of factor V Leiden, 0.7 (95% confidence interval, 0.4 to 1.2) for prothrombin G20210A, 1.2 (95% confidence interval, 0.5 to 2.6) for homozygous carriers of factor V Leiden and/or prothrombin G20210A, and 1.0 (95% confidence interval, 0.6 to 1.9) for double heterozygotes of both mutations. Adjustments for age, sex, family status, first event type, and concomitance of natural anticoagulant deficiencies did not alter the risk estimates. In this study, individuals with homozygous factor V Leiden and/or homozygous prothrombin G20210A or double heterozygous carriers of factor V Leiden and prothrombin G20210A did not have a high risk of recurrent venous thrombosis.

  4. The frequency of factor V Leiden and prothrombin G20210A mutations in Slovak and Roma (Gypsy) ethnic group of Eastern Slovakia.

    Science.gov (United States)

    Bôžiková, Alexandra; Gabriková, Dana; Sovičová, Adriana; Behulová, Regina; Mačeková, Soňa; Boroňová, Iveta; Petrejčíková, Eva; Soták, Miroslav; Bernasovská, Jarmila; Bernasovský, Ivan

    2012-10-01

    Factor V Leiden and prothrombin G20210A are the two most prevalent causes of inherited thrombophilia. The prevalence of these mutations varies widely in healthy Caucasian population. The aim of our study was to determine the frequency of factor V Leiden and prothrombin G20210A mutations in Slovak and Roma ethnic group from Eastern Slovakia. We analyzed 540 asymptomatic individuals (269 individuals of Slovak ethnicity and 271 individuals of Roma ethnicity) by real-time PCR method. The detected allele frequencies were 2.97 versus 6.64 % for factor V Leiden (p = 0.0049), and 0.74 versus 0.92 % for prothrombin mutation (p = 0.7463) in Slovak and Roma population, respectively. The Roma ethnic group had significantly higher prevalence of factor V Leiden mutation when compared to Slovak ethnic group. The allele frequency of factor V Leiden in ethnic Romanies from Eastern Slovakia was one of the highest in Europe. Our results confirm an uneven geographical and ethnic distribution of factor V Leiden.

  5. Atypical presentation of livedo racemosa in a factor V Leiden heterozygous positive patient with Pseudomonas aeruginosa urosepsis.

    Science.gov (United States)

    Johnston, Jesse; Noakes, Rowland; Davidson, Kurt

    2016-11-12

    Impairment of the protein C pathway, detectable by reduced plasma levels of activated protein C (APC), are risk factors for venous thrombosis. Activated protein C maintains clotting homeostasis by regulation of pro-coagulant factors Va and VIIIa. Both infection and the factor V Leiden mutation reduce the formation of APC from protein C in the blood. With low levels of APC, excess factors Va and VIIIa exist, increasing the risk of thrombus formation. Livedo racemosa is characterised by a striking, violaceous branch-like pattering of the skin. It is similar to livedo reticularis, but with a different morphology and histopathology. In this case report we present the first case of livedo racemosa, in an 89-year-old factor V Leiden-positive patient with a Pseudomonas aeruginosa urinary tract infection. The cutaneous biopsies demonstrated vasculopathy with intraluminal thrombi in subcutaneous vessels with no evidence of inflammatory vasculitis.

  6. Telomere length and anaemia in old age: results from the Newcastle 85-plus Study* and the Leiden 85-plus Study

    Science.gov (United States)

    Den Elzen, Wendy P. J.; Martin-Ruiz, Carmen; von Zglinicki, Thomas; Westendorp, Rudi G. J.; Kirkwood, Thomas B. L.; Gussekloo, Jacobijn

    2011-01-01

    Background: reduced telomere length in blood cells has been associated with increased risk of multiple age-related diseases and is widely regarded as a general biomarker of ageing. Therefore, it is important to know both the extent and limitations of this association. We investigated the relation between telomere length and anaemia in two independent cohorts, with the prior expectation of adding anaemia to the list of conditions for which telomere reduction is a risk factor. Participants and methods: the present study is embedded in the Newcastle 85-plus Study and Leiden 85-plus Study, two population-based studies of inhabitants of Newcastle and North Tyneside, UK (n = 749) and Leiden, the Netherlands (n = 658) aged 85 and over. High-molecular-weight DNA was isolated from full fresh blood (Newcastle) and peripheral blood mononuclear cells samples (Leiden). Telomere length was measured as abundance of telomeric template versus a single gene by quantitative real-time polymerase chain reaction. Anaemia was defined according to World Health Organization criteria. Results: in both studies, no differences in median telomere length were observed between participants with anaemia and participants without anaemia (Newcastle: 2,846 bp (interquartile range (IQR) 2,433–3,630) versus 2,920 bp (IQR 2,425–3,570), P = 0.63; Leiden: 4,136 bp (IQR 3,879–4,428) versus 4,167 bp (IQR 3,893–4,501), P = 0.41). Telomere length also did not correlate with any other haematological parameter in both men and women. Conclusions: in contrast to other age-related diseases, telomere length is not associated with anaemia or any other haematological parameter in older individuals in the general population. PMID:21622673

  7. Risk of venous thromboembolism and myocardial infarction associated with factor V Leiden and prothrombin mutations and blood type

    DEFF Research Database (Denmark)

    Sode, Birgitte F; Allin, Kristine H; Dahl, Morten

    2013-01-01

    ABO blood type locus has been reported to be an important genetic determinant of venous and arterial thrombosis in genome-wide association studies. We tested the hypothesis that ABO blood type alone and in combination with mutations in factor V Leiden R506Q and prothrombin G20210A is associated...... with the risk of venous thromboembolism and myocardial infarction in the general population....

  8. Factor V Leiden, Prothrombin and MTHFR Mutation in Patients with Preeclamsia, Intrauterine Growth Restriction and Placental Abruption.

    Science.gov (United States)

    Livrinova, Vesna; Lega, Marija Hadzi; Dimcheva, Anita Hristova; Samardziski, Igor; Isjanovska, Rozalinda

    2015-12-15

    Factor V Leiden, Prothrombin and MTHFR gene mutation, could have an influence in pregnancy with adverse outcome Preeclamsia, IUGR and Placental abruption. The aim of this study is to investigate the presence of above mentioned inherited thrombophilias and its statistical significance, distribution among the complicated and normal pregnancy, and relative risk for carrier of mutation to develop preeclampsia, IUGR and placental abruption. Prospective cohort study is implemented at University Clinic for Obstetric and Gynecology in Skopje, Republic of Macedonia. The study included 109 delivered patients: 40 with preeclapmsia, 22 with IUGR, 17 with placental abruption and 30 as control group with normal pregnancy. The amount of 3 ml venous blood has been used for detection of these point mutations using ThromboStrip -Opegen, QIAGEN kit manufactured for thrombotic risk. The highest frequency was found: in the group with preeclampsia 35% were MTHFR homozygous, IUGR -MTHFR heterozygous 45%, Placental abruption- 52.9% MTHFR heterozygous, and in the control group without thrombophilia 56.7%. There were combined thrombophilia in 3 patients. There aren`t statistical significance in presence of thrombophilia among groups (p > 0.05). Statistical significance (p Factor V Leiden heterozygous was 4.50 (0.47Factor V Leiden for placental abruption. Further investigations with more patients are warranted.

  9. To determine the frequency of Factor V Leiden in cases of Deep Vein Thrombosis and Healthy controls.

    Science.gov (United States)

    Saeed, Anjum; Sumreen; Kashif, Muhammad Ali

    2015-01-01

    To determine the frequency of Factor V Leiden in cases of Deep Vein Thrombosis and Healthy controls. This case control study was performed in Armed Forces Institute of Pathology Rawalpindi, From 21(st) March to 25(th) September 2013. One hundred patients with diagnostic evidence of Deep vein thrombosis on Doppler ultrasound/Magnetic resonance imaging (MRI) scan were included in the study through non probability convenient sampling and compared with 100 matched healthy controls. DNA was extracted from the blood sample by kit method. In order to identify Factor V Leiden mutation, the polymerase chain reaction (PCR) method was utilized combined with the Amplification refractory mutation system. Data was analyzed using statistical package for social sciences (SPSS) version 17. In 100 patients of Deep Vein Thrombosis (DVT), frequency of Factor V Leiden (FVL) was 13% and it is was 2% in healthy control group. A significant association was found between FVL and DVT with odds ratio of 7.32 and with P value (P = 0.003). FVL was found to be highly prevalent among patients of DVT, Signifying strong association between the two.

  10. Possible incorrect genotyping of heterozygous factor V Leiden and Prothrombin 20210 gene mutations by the GeneXpert assay.

    Science.gov (United States)

    Marturano, Alessandro; Bury, Loredana; Gresele, Paolo

    2014-08-05

    The GeneXpert analyzer is a hands-off system for the detection of Factor V Leiden and of Prothrombin G20210A (GPRO) gene thrombophilic mutations. Although the system is efficient and easy to use, we report the rare possibility of incorrect genotyping. 1648 samples were evaluated using the GeneXpert HemosIL Factor II and Factor V assay: 1319 were freshly analyzed while 329 were frozen, thawed and diluted with saline prior to analysis to avoid clogging of the instrument syringe. Two samples, both heterozygous, one for the factor V Leiden and the other for the GPRO gene, were incorrectly genotyped as homozygous for the relative mutation. Inspection of the Ct values and amplification curves and genotyping with PCR revealed the correct genotype as heterozygous for factor V Leiden and GPRO mutation. The GeneXpert HemosIL Factor II and Factor V assay is an automated, fast genotyping assay requiring almost no sample manipulation, advantageous characteristics if compared with other PCR-based methods. However, an inattentive use of it can generate incorrect diagnosis. A careful handling of the sample, in particular correct dilution of frozen/thawed samples before analysis, and the inspection of the amplification curves and Ct values are required to avoid artifacts. Copyright © 2014 Elsevier B.V. All rights reserved.

  11. Vermeer, de Delft, cartographe

    Directory of Open Access Journals (Sweden)

    Georges KAMPEN

    1986-09-01

    Full Text Available Le tiers des tableaux du grand peintre hollandais (dont «Le géographe» présente une carte ou une mappemonde. Cela traduit à la fois le goût de la société du temps pour une représentation minutieuse de la réalité et l'importance de la carte dans une période troublée de l'histoire des Pays-Bas.

  12. The frequencies of FV Leiden and FII G20210A mutations in patients with different clinical manifestations of venous thromboembolism: Experience from large Serbian cohort

    Directory of Open Access Journals (Sweden)

    Tomić Branko

    2016-01-01

    Full Text Available Venous thromboembolism is a multifactorial disorder with two manifestations: deep-vein thrombosis and pulmonary embolism. Pulmonary embolism is usually considered as the complication of deep-vein thrombosis, but there are reported cases of isolated pulmonary embolism. FV Leiden and FII G20210A mutations are most common genetic risk factors for the venous thromboembolism. Several studies reported "FV Leiden paradox": lower prevalence of FV Leiden mutation among patients with isolated pulmonary embolism than among those with deep-vein thrombosis. The aim of this study was to determine FV Leiden and FII G20210A mutations frequency in thrombophilic patients in Serbian population. We tested prevalence of these mutations carriers in 1427 individuals divided in three groups of patients (with deep-vein thrombosis, deep-vein thrombosis/ pulmonary embolism and isolated pulmonary embolism and control group. All subjects were tested for these mutations using PCR-RFLP analysis. Detected frequency of FV Leiden heterozygous carriers in patients with isolated pulmonary embolism was 6.9% (for FII G20210A 11.6%, while in other two groups of patients with deep-vein thrombosis and deep vein thrombosis/pulmonary embolism, frequency was 18.6% (for FII G20210A mutation were 11.6% and 8.3%, respectively. Our results showed that FV Leiden mutation is less frequent in patients with isolated pulmonary embolism than in patients with deep-vein thrombosis or deep-vein thrombosis accompanied with pulmonary embolism, confirming "FV Leiden paradox". On the other hand, detected frequency of FII G20210A mutation carriers was similar in all three groups of patients. [Projekat Ministarstva nauke Republike Srbije, br. 173008

  13. Science and taste. Painting, passions, and the new philosophy in seventeenth-century Leiden.

    Science.gov (United States)

    Smith, P H

    1999-09-01

    This article argues that the art collection owned by Franciscus dele Boë, Sylvius, a professor of practical medicine at the University of Leiden from 1658 to 1672, gives insight into some aspects of the character and significance of the new philosophy in the midseventeenth century. Through his teaching, his advocacy, and his practice of the new experimental philosophy, Sylvius played a role in shaping and institutionalizing the practices of the new philosophy that spread throughout Europe in the late seventeenth and eighteenth centuries. Sylvius's house design and large painting collection also exemplified the consumption and taste of the northern Netherlands in the seventeenth century. An examination of both Sylvius's science and his taste can help us understand what was at stake for Sylvius and his contemporaries in their practice of the new philosophy. This article finds that Sylvius's taste and his science both involved practices of social distinction, demarcation, and control. Moreover, both were enmeshed in controversy about the epistemological status of knowledge gained through the senses and about the practices by which that knowledge was gathered.

  14. Factor V leiden and ischemic stroke risk: the Genetics of Early Onset Stroke (GEOS) study.

    Science.gov (United States)

    Hamedani, Ali G; Cole, John W; Cheng, Yuching; Sparks, Mary J; O'Connell, Jeffrey R; Stine, Oscar C; Wozniak, Marcella A; Stern, Barney J; Mitchell, Braxton D; Kittner, Steven J

    2013-05-01

    Factor V Leiden (FVL) has been associated with ischemic stroke in children but not in adults. Although the FVL mutation is associated with increased risk for venous thrombosis, its association with ischemic stroke in young adults remains uncertain. Therefore, we examined the association between FVL and ischemic stroke in participants of the Genetics of Early Onset Stroke (GEOS) study. A population-based case control study identified 354 women and 476 men 15 to 49 years of age with first-ever ischemic stroke and 907 controls. Participant-specific data included vascular risk factors, FVL genotype and, for cases, the ischemic stroke subtype by modified Trial of ORG 10172 in Acute Stroke criteria. Logistic regression was used to calculate odds ratios for the entire population and for subgroups stratified by risk factors and ischemic stroke subtype. The frequency of the FVL mutation was similar between ischemic stroke patients (3.6%; 95% confidence interval [CI] 2.5%-5.1%) and nonstroke controls (3.8%; 95% CI 2.7%-5.2%). This frequency did not change significantly when cases were restricted to patients with stroke of undetermined etiology (4.1%; 95% CI 2.6%-6.4%). Among young adults, we found no evidence for an association between FVL and either all ischemic stroke or the subgroup with stroke of undetermined etiology. Published by Elsevier Inc.

  15. Leiden Index of Depression Sensitivity-Revised (LEIDS-R: Spanish validation proposal

    Directory of Open Access Journals (Sweden)

    Cristina Senín-Calderón

    2017-01-01

    Full Text Available Adaptar y validar al espa˜nol la escala Leiden de Sensibilidad para la Depresión Revisada para evaluar la reactividad cognitiva al humor depresivo. Conocer la estructura factorial de la escala y hallar las propiedades psicométricas. La muestra consistió en 600 participantes (103 pacientes y 497 sujetos de población general. Se obtuvo una estructura de cuatro factores, se propuso un factor general que evalúa la reactividad cognitiva y se eliminaron 10 ítems. Se propone una versión reducida de la escala (LEIDS-R24. Los factores y la escala global presentan una adecuada consistencia interna y los resultados de la validación muestran que todos los factores de la escala predicen adecuadamente la sintomatología depresiva (BDI-II y muestran correlaciones elevadas con la Escala de Actitudes Disfuncionales y BDI-II. La escala completa discriminó adecuadamente en sintomatología depresiva entre sujetos de la población general y sujetos con trastornos de ansiedad y depresivos. No se hallaron diferencias significativas en la medida LEIDS-R24 entre sujetos con trastornos de ansiedad y depresivos. La escala LEIDS-R24 puede ser una medida útil y breve para evaluar la reactividad cognitiva al humor depresivo y analizar la vulnerabilidad común que puede darse entre personas con trastornos de ansiedad y depresivos.

  16. Factor V-Leiden Mutation: A Common Risk Factor for Venous Thrombosis among Lebanese Patients

    Directory of Open Access Journals (Sweden)

    Raghid Kreidy

    2012-01-01

    Full Text Available Aim. Lebanon exhibits one of the highest prevalences of factor V-Leiden (FVL in the world (14.4%. The aim of this study is to evaluate the incidence of FVL mutation among Lebanese patients with lower extremity venous thrombosis. Material and Methods. From January 2003 to January 2011, 283 consecutive Lebanese patients, diagnosed with deep venous thrombosis (DVT by duplex scan, were retrospectively reviewed. FVL mutation was tested among patients with conditions highly suggestive of hypercoagulation states (65 patients. Results. FVL mutation was detected among 56.9% of patients, 68.6% of patients younger than 50 years, and 43.4% of patients older than 50 years (=0.041. FVL mutation was commonly reported in young adults, in patients with pregnancy, estrogen drugs, recurrent DVT, and resistance to anticoagulation. Conclusion. The high rate of FVL mutation observed among Lebanese patients with venous thrombosis is related to the high prevalence of this mutation in the Lebanese population. Thrombophilia screening should be tailored to accommodate a population's risk factor. In countries with high prevalence of FVL, this mutation should be screened among patients younger than 50 years and patients with situations highly suggestive of hypercoagulation states.

  17. Factor V Leiden: should we screen oral contraceptive users and pregnant women?

    Science.gov (United States)

    Vandenbroucke, J. P.; van der Meer, F. J.; Helmerhorst, F. M.; Rosendaal, F. R.

    1996-01-01

    The factor V Leiden mutation is the most common genetic risk factor for deep vein thrombosis: it is present in about 5% of the white population. The risk of deep vein thrombosis among women who use oral contraceptives is greatly increased by the presence of the mutation. The same seems to be true of the risk of postpartum thrombosis. Several authors have called for all women to be screened before prescription of oral contraceptives and during pregnancy. Such a policy might deny effective contraception to a substantial number of women while preventing only a small number of deaths due to pulmonary emboli. Moreover, in pregnancy the ensuing use of oral anticoagulation prophylaxis might carry a penalty of fatal bleeding that is equal to or exceeds the risk of death due to postpartum thrombosis. It might pay, however, to take a personal and family history of deep vein thrombosis when prescribing oral contraceptives or at a first antenatal visit to detect women from families with a tendency to multiple thrombosis. Images p1129-a PMID:8916702

  18. Lack of association between factor V Leiden and sepsis: a meta-analysis.

    Science.gov (United States)

    Zhang, Jing; He, Yanxian; Song, Weibing; Lu, Yong; Li, Ping; Zou, Li; Zhong, Wuzhuang

    2015-04-01

    Some studies evaluated the association of factor V Leiden (FVL) with sepsis risk and mortality risk. However, the results were conflicting. Thus, we performed a meta-analysis to address the association between FVL and sepsis. PubMed and EMBASE databases were searched to find relevant studies. Odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were estimated using random effects model. Five case-control studies and 3 cohort studies were included. Overall, no significant association between FVL and sepsis risk was observed (OR = 0.93; 95% CI 0.74-1.15; P = .49). In addition, there was no significant association between FVL and sepsis-related mortality (OR = 1.17; 95% CI 0.73-1.88; P = .52). In the subgroup analysis, no increased sepsis risk and mortality risk were found in caucasian population. This meta-analysis suggested that FVL was not a risk factor for sepsis and sepsis mortality. © The Author(s) 2013.

  19. Hepatocyte-specific IKKβ expression aggravates atherosclerosis development in APOE*3-Leiden mice

    Science.gov (United States)

    Wong, Man C.; van Diepen, Janna A.; Hu, Lihui; Guigas, Bruno; de Boer, Hetty C.; van Puijvelde, Gijs H.; Kuiper, Johan; van Zonneveld, Anton J.; Shoelson, Steven E.; Voshol, Peter J.; Romijn, Johannes A.; Havekes, Louis M.; Tamsma, Jouke T.; Rensen, Patrick C.N.; Hiemstra, Pieter S.; Berbée, Jimmy F.P.

    2014-01-01

    Objective The liver is the key organ involved in systemic inflammation, but the relation between hepatic inflammation and atherogenesis is poorly understood. Since nuclear factor-κB (NF-κB) is a central regulator of inflammatory processes, we hypothesized that chronically enhanced hepatic NF-κB activation, through hepatocyte-specific expression of IκB kinase-β (IKKβ) (LIKK), will aggravate atherosclerosis development in APOE*3-Leiden (E3L) mice. Methods and Results E3L.LIKK and E3L control littermates were fed a Western-type diet for 24 weeks. E3L.LIKK mice showed a 2.3-fold increased atherosclerotic lesion area and more advanced atherosclerosis in the aortic root with less segments without atherosclerotic lesions (11 vs. 42%), and more segments with mild (63% vs. 44%) and severe (26% vs. 14 %) lesions. Expression of LIKK did not affect basal levels of inflammatory parameters, but plasma cytokine levels tended to be higher in E3L.LIKK mice after lipopolysaccharide (LPS) administration. E3L.LIKK mice showed transiently increased plasma cholesterol levels, confined to (V)LDL. This transient character resulted in a mild (+17%) increased cumulative plasma cholesterol exposure. Conclusion We conclude that selective activation of NF-κB in hepatocytes considerably promotes atherosclerosis development which is (at least partly) explained by an increased sensitivity to proinflammatory triggers and transiently increased plasma cholesterol levels. PMID:21798539

  20. Primary thrombophilia in Mexico. II. Factor V G1691A (Leiden), prothrombin G20210A, and methylenetetrahydrofolate reductase C677T polymorphism in thrombophilic Mexican mestizos.

    Science.gov (United States)

    Ruiz-Argüelles, G J; Garcés-Eisele, J; Reyes-Núñez, V; Ramírez-Cisneros, F J

    2001-01-01

    We have shown that in Mexican mestizo patients with clinical features of primary thrombophilia, 39% have activated protein C resistance phenotype, 5% protein C deficiency, and 2% protein S deficiency. In the present study, in a group of 37 thrombophilic Mexicans and 50 normal controls, we assessed the factor V G1691A (Leiden), the prothrombin G20210A, and the methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphisms. Four patients were found to be heterozygous for factor V Leiden, 5 heterozygous for the prothrombin 20210, 16 heterozygous, and 6 homozygous for the MTHFR 677. There were four individuals with co-segregation of alleles: two heterozygotes for the factor V Leiden/prothrombin 20210, one heterozygote for prothrombin 20210/MTHFR 677, and one heterozygote for prothrombin 20210/homozygote for MTHFR 677. For factor V Leiden, prothrombin 20210, and MTHFR 677 mutations, the allele frequencies were respectively 1% (+/-0.2%, alpha = 0.05), 7.66 (P mestizo thrombophilic patients, the low prevalence of the factor V Leiden mutation (10.8%) and the high prevalence of the prothrombin 20210 mutation (13.5%) contrast with those identified in Caucasian thrombophilic patients (21% and 6%, respectively; P mestizo patients.

  1. Case control study of the factor V Leiden and factor II G20210A mutation frequency in women with recurrent pregnancy loss.

    Science.gov (United States)

    Teremmahi Ardestani, Majid; Nodushan, Hossein Hadi; Aflatoonian, Abbas; Ghasemi, Nasrin; Sheikhha, Mohammad Hasan

    2013-01-01

    Recurrent pregnancy loss (RPL) caused by various genetic and non-genetic factors. After chromosome abnormality, thrombophilia is one of the most important genetic factors that could cause RPL. Factor V Leiden and factor II G20210A mutation were the most common mutations cause thrombophilia in the world. The purpose of this study was to determine the frequency of factor V Leiden and prothrombine gene mutations in women with RPL compared with women who had uneventful pregnancies. This case control study evaluates the frequency of factor V-Leiden and factor II G20210 genotypes in 80 women with two or more pregnancy losses, compared with 80 women without adverse pregnancy outcome. The mutations were assessed by PCR-RFLP. Frequency of the factor V Leiden among cases was 2.5%, which was higher than controls (1.25%), but the difference was not significant. No factor II G20210 mutation was found among cases and controls. These data did not confirm that factor V Leiden and factor II G20210 mutation might play a role in recurrent pregnancy loss in Iranian women.

  2. A Multiplex Allele Specific Polymerase Chain Reaction (MAS-PCR) for the Detection of Factor V Leiden and Prothrombin G20210A.

    Science.gov (United States)

    Bagheri, Morteza; Rad, Isa Abdi

    2011-01-01

    In order to determine the frequencies of factor V Leiden and prothrombin G20210A point mutations in the Iranian population with Azeri Turkish origin. 120 unrelated individuals from general population randomly selected and were examined for factor V Leiden and prothrombin G20210A mutations using a multiplex allele specific polymerase chain reaction (MAS-PCR) assayOutcomes: The frequency of prothrombin G20210A mutation was 2.08%, which means 5 chromosomes out of 240 chromosomes had prothrombin G20210A mutation. The distribution of prothrombin 20210 GG, GA, AA genotypes and prothrombin 20210A allele were 37(92.5%), 3(7.5%), 0(0%) and 3(3.75%) in males and 78(97.5%), 2(2.5%), 0(0%) and 2(1.25%) in females, respectively. Factor V Leiden was not found in our tested group (zero chromosomes out of 240 chromosomes). Analysis of the observed frequencies in the studied groups indicates that there is no statistically significant difference between females and males, regarding prothrombin G20210A mutation (p value>0.05). This is the first study in its own kind in this population and implies that the frequency of Factor V Leiden G1691A (R506Q, FV-Leiden) allele is extremely low but the prothrombin G20210A mutation is more frequent in the tested group.

  3. Factor V Leiden 1691G/A and prothrombin gene 20210G/A polymorphisms as prothrombotic markers in adult Egyptian acute leukemia patients.

    Science.gov (United States)

    El Sissy, Azza Hamdy; El Sissy, Maha H; Elmoamly, Shereef

    2014-11-01

    Factor V Leiden 1691G/A and prothrombin gene 20210G/A mutations are the most common genetic defects leading to thrombosis. This work aimed to study the FV Leiden and the prothrombin gene polymorphism in adult Egyptian patients with acute leukemia and their importance in thrombophilia screening. The study included 76 patients with acute leukemia and 100 healthy controls. Genotyping was done by real-time polymerase chain reaction technique. For factor V Leiden, the frequency of G/A mutation conferred more than 2.5-fold of increased risk of (OR 2.639 95 % CI 1.045-6.669). The frequency of factor V Leiden combined (G/A + A/A) genotypes conferred 2.83-fold of increased risk (OR 2.828, CI 1.13-7.075), The A allele conferred almost threefold increased risk (OR 2.824, 95 % CI 1.175-6.785). Despite higher frequency in patients compared to controls, there was no risk of association between prothrombin gene mutation and acute leukemia in adult Egyptians nor was there between combined genotypes of prothrombin gene mutation and factor V Leiden.

  4. Exploring the use of automated vehicles as last mile connection of train trips through an agent-based simulation model: An application to Delft, Netherlands

    Directory of Open Access Journals (Sweden)

    Arthur Scheltes

    2017-06-01

    Full Text Available The last mile in a public transport trip is known to bring a large disutility for passengers, because the conventional transport modes for this stage of the trip can, in many cases, be rather slow, inflexible and not provide a seamless experience to passengers. Fully automated vehicles (AVs, that is, those which do not need a driver, could act as a first mile/last mile connection to mass public transport modes. In this paper, we study a system that we call Automated Last-Mile Transport (ALMT, which consists of a fleet of small, fully automated, electric vehicles to improve the last mile performance of a trip done in a train. An agent-based simulation model was proposed for the ALMT whereby a dispatching algorithm distributes travel requests amongst the available vehicles using a FIFO sequence and selects a vehicle based on a set of specified control conditions (e.g. travel time to reach a requesting passenger. The model was applied to the case-study of the connection between the train station Delft Zuid and the Technological Innovation Campus (Delft, The Netherlands in order to test the methodology and understand the performance of the system in function of several operational parameters and demand scenarios. The most important conclusion from the baseline scenario was that the ALMT system was only able to compete with the walking mode and that additional measures were needed to increase the performance of the ALMT system in order to be competitive with cycling. Relocating empty vehicles or allowing pre-booking of vehicles led to a significant reduction in average waiting time, whilst allowing passengers to drive at a higher speed led to a large reduction in average travel time, whilst simultaneously reducing system capacity as energy use is increased.

  5. Analytic validity of genetic tests to identify factor V Leiden and prothrombin G20210A.

    Science.gov (United States)

    Emadi, Ashkan; Crim, Matthew T; Brotman, Daniel J; Necochea, Alejandro J; Samal, Lipika; Wilson, Lisa M; Bass, Eric B; Segal, Jodi B

    2010-04-01

    The objective of this study is to systematically review methods for detecting Factor V Leiden or prothrombin G20210A. English-language literature from MEDLINE, EMBASE, The Cochrane Library, the Cumulative Index to Nursing and Allied Health Literature, PsycInfo(c), 2000-December 2008. Studies assessed methods for detection of these mutations in at least 10 human blood samples and reported concordance, discordance, or reproducibility. Two investigators abstracted data on the sample selection criteria, test operators, DNA extraction, experimental test, reference standard, commercial instruments, concordance rates, explanation of any discordance, and whether discordance resolved after repetition. We assessed strength of the evidence using the GRADE criteria. We reviewed 7,777 titles and included 66 articles. The majority of the reviewed studies used PCR-RFLP or AS-PCR as the reference standard. The studies demonstrated that commercially available and precommercial tests have high analytic validity with all having greater than 99% concordance with the reference standard. With a few exceptions, discordance resolved with repetition of the test, suggesting operator or administrative errors were responsible for the discordant results. In the quality assurance studies, greater than 98% of laboratories demonstrated high, even perfect, accuracy when asked to diagnose a sample with a known mutation. The majority of errors came from a limited number of laboratories. Although not all methods may be accurate, there is high-grade evidence that genetic tests for the detection of FVL and prothrombin G20210A have excellent analytic validity. There is high-grade evidence that most, but not all, clinical laboratories test for FVL and prothrombin G20210A accurately.

  6. Motivating factors for physician ordering of factor V Leiden genetic tests.

    Science.gov (United States)

    Hindorff, Lucia A; Burke, Wylie; Laberge, Anne-Marie; Rice, Kenneth M; Lumley, Thomas; Leppig, Kathleen; Rosendaal, Frits R; Larson, Eric B; Psaty, Bruce M

    2009-01-12

    The factor V Leiden (FVL) genetic test is used by many physicians despite its uncertain clinical utility. We investigate whether self-reported motivations and behaviors concerning FVL genetic testing differ between 2 groups of primary care physicians defined by frequency of previous FVL test use. In January 2007, 112 physicians (60 frequent and 52 infrequent FVL test users) at Group Health, a large health care delivery system, were surveyed. Survey content areas included primary reasons and motivating factors for ordering the FVL test, the likelihood of ordering the FVL test for hypothetical patients, potential barriers to genetic testing, and practices and skills regarding FVL test ordering. Responses between groups agreed concerning most clinical- and patient-related factors. Frequent-FVL physicians were more likely than infrequent-FVL physicians to report ordering the FVL test for hypothetical patients with mesenteric venous thrombosis (adjusted odds ratio, 4.57; 95% confidence interval, 1.55-13.53) or venous thrombosis after hospital discharge (adjusted odds ratio, 3.42; 95% confidence interval, 1.30-8.95). Frequent-FVL physicians were also less likely to identify several items on the survey as barriers to genetic testing and were more likely to report high confidence in interpreting and explaining FVL test results. Generally, both physician groups reported similar motivating factors for ordering FVL tests, and reported behaviors were consistent with existing guidelines. More striking differences were observed for measures such as barriers to and confidence in using genetic tests. Although additional research is necessary to evaluate the impact of these results, they inform several knowledge-to-practice translation issues that are important for the successful integration of genetic testing into primary care.

  7. Motivating factors for physician ordering of Factor V Leiden genetic tests

    Science.gov (United States)

    Hindorff, Lucia A.; Burke, Wylie; Laberge, Anne-Marie; Rice, Kenneth M.; Lumley, Thomas; Leppig, Kathleen; Rosendaal, Frits R.; Larson, Eric B.; Psaty, Bruce M.

    2009-01-01

    Background The Factor V Leiden (FVL) genetic test is used by many physicians despite its uncertain clinical utility. This study investigated whether self-reported motivations and behaviors concerning FVL genetic testing differed between two groups of primary care physicians defined by frequency of prior FVL test use. Methods In January 2007, 112 primary care physicians (60 frequent, 52 infrequent FVL test users) at Group Health, a large health care delivery system, were surveyed. Survey content areas included: primary reasons and motivating factors for ordering FVL; likelihood of ordering FVL for hypothetical patients; potential barriers to genetic testing, and practices and skills regarding FVL test ordering. Results Responses between groups agreed concerning most clinical- or patient-related factors. Frequent-FVL physicians were more likely than infrequent-FVL physicians to report ordering FVL for hypothetical patients with mesenteric venous thrombosis (adjusted OR 4.57, 95% CI 1.55, 13.53) or venous thrombosis following hospital discharge (adjusted OR 3.42, 95% CI 1.30, 8.95). Frequent-FVL physicians were also less likely to agree with several potential barriers to genetic testing and more likely to report high confidence in interpreting and explaining FVL test results. Conclusions Generally, both groups of physicians reported similar motivating factors for ordering FVL, and reported behaviors were consistent with existing guidelines. More striking differences were observed for measures such as barriers to and confidence in using genetic tests. Though additional research is necessary to evaluate their impact, these results inform several knowledge-to-practice translation issues that are important to the successful integration of genetic testing into primary care. PMID:19139326

  8. The association between Factor V Leiden with the presence and severity of coronary artery disease.

    Science.gov (United States)

    Boroumand, Mohammadali; Pourgholi, Leila; Ziaee, Shayan; Anvari, Maryam Sotoudeh; Jalali, Arash; Goodarzynejad, Hamidreza

    2014-04-01

    The presence of Factor V Leiden (FVL) is proposed to be associated with a higher risk for arterial thrombosis. The aim of this study was to examine a relationship between FVL with the presence and severity of angiographically determined coronary artery disease (CAD). In this case-control study, 1083 patients having angiographic evidence of atherosclerosis with ≥50% luminal stenosis in their epicardial coronary tree were compared with patients with no luminal stenosis (n=320) or with luminal stenosis Factor V polymorphisms was analyzed using polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). FVL was found to be independently associated with the occurrence of CAD (p=0.020). As compared to wild genotype, heterozygote or homozygote mutant genotypes were more likely associated with a trend towards more severe CAD (adjusted OR=1.85, 95% CI=1.26 to 2.72; p=0.002, and adjusted OR=3.70, 95% CI=1.71 to 8.00; p=0.001; respectively). In addition, the median and inter-quartile range for Gensini score were significantly different among the GG (27.8, 3 to 66.5), GA (53.5, 10 to 104.1), and AA (92.8, 48.1 to 125.9) genotypes (p<0.001). Our results confirmed the hypothesis that FVL mutation is a significant determinant of CAD risk. Furthermore, we observed that FVL is independently associated with increasing CAD severity. Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  9. Phenotypic presentation of thrombophilia in double heterozygote for factor v leiden and prothrombin 20210 G>A mutations: Case report

    Directory of Open Access Journals (Sweden)

    Nagorni-Obradović Ljudmila

    2014-01-01

    Full Text Available Physicians usually do not suspect pulmonary thromboembolism in younger patients except in those who have thrombophilia. In those latter patients some special conditions such as trauma or surgery may provoke the disease. In some adult persons, thrombophilia may still remain unrecognized, until appearance of additional conditions influence development of thrombosis. A 55-year-old Caucasian female, non-smoker, experienced sudden chest pain and hemoptysis without chest trauma. History taking revealed type 2 diabetes mellitus and hypothyroidism. She was overweight with body mass index 29.0. The review of the family history revealed that her father and mother died of brain infarction, while her 22-year-old son and 24-year-old daughter were healthy. Due to suspicion for thrombosis, multi-slice computerized tomography thorax scan was done and pulmonary embolism was diagnosed. Although without clear risk factor for thrombosis in our patient, we performed laboratory investigation for congenital thrombophilia. Genetic analysis showed double heterozygous for factor V Leiden and prothrombin 20210 G>A mutations. Congenital thrombophilia was risk factor for thrombosis in our patient but haemostatic imbalance was not previously clinically recognized. She had two pregnancies without complications. Appearance of other associative factors such as endocrine disorders - hypothyroidism and metabolic syndrome with diabetes type 2, and overweigh were additional potential triggers for clinical manifestation of pulmonary thromboembolism in her adult age. Her children underwent genetic analysis, too. The son was also double heterozygous for factor V Leiden and prothrombin 20210 G>A mutations, while daughter was heterozygous for factor V Leiden, and none had clinical signs of thrombosis. [Projekat Ministarstva nauke Republike Srbije, br. ON175081 i br. ON 175091

  10. Relationship between Factor V Leiden Gene Variant and Risk of Ischemic Stroke: A Case-Control Study.

    Science.gov (United States)

    Kumar, Amit; Misra, Shubham; Sagar, Ram; Kumar, Pradeep; Yadav, Arun K; Talwar, Pumanshi; Raj, Ritesh; Prasad, Kameshwar

    2017-01-01

    Factor V Leiden is the most common genetic variation among the blood coagulation pathway which leads to prothrombotic state, therefore, is considered an important gene for understating the stroke mechanism. The aim of the present study is to determine the relationship between single nucleotide polymorphism at G1691A position of Factor V gene and risk of ischemic stroke (IS) in North Indian population. In a retrospective case-control study, 250 patients with IS and 250 age- and gender-matched controls were enrolled in the period of October 2012 to September 2014 from in- and out-patient department of Neurology, All India Institute of Medical Sciences, New Delhi, India. Deoxyribonucleic acid for each case and control was isolated from peripheral blood using phenol-chloroform extraction method. Polymerase chain reaction-restriction fragment length polymorphism method was used to determine the polymorphism. Data were analyzed using STATA Software Version 13. The mean age of IS patient was 52.8 ± 12.5 years and in control group was 50.97 ± 12.7 years. Genotypic frequency distributions were in accordance with Hardy-Weinberg equilibrium in both cases and controls. As expected hypertension, diabetes, dyslipidemia, smoking, heavy alcohol intake, family history of stroke, and poor economic status were significantly associated with the risk of IS. Multivariate analysis revealed 5.17 times higher odds for developing the risk of large vessel subtype of IS in patients carrying Factor V Leiden G1691A gene variation as compared to control subjects (OR, 5.17; 95% CI, 1.32-20.3, P = 0.01). The present study suggests that Factor V Leiden G1691A polymorphism may be significantly associated with the risk of large vessel subtype of IS. Large sample size studies using prospective cohort designs are required to corroborate the present findings.

  11. Relationship between Factor V Leiden Gene Variant and Risk of Ischemic Stroke: A Case–Control Study

    Science.gov (United States)

    Kumar, Amit; Misra, Shubham; Sagar, Ram; Kumar, Pradeep; Yadav, Arun K; Talwar, Pumanshi; Raj, Ritesh; Prasad, Kameshwar

    2017-01-01

    Background: Factor V Leiden is the most common genetic variation among the blood coagulation pathway which leads to prothrombotic state, therefore, is considered an important gene for understating the stroke mechanism. Aim: The aim of the present study is to determine the relationship between single nucleotide polymorphism at G1691A position of Factor V gene and risk of ischemic stroke (IS) in North Indian population. Materials and Methods: In a retrospective case–control study, 250 patients with IS and 250 age- and gender-matched controls were enrolled in the period of October 2012 to September 2014 from in- and out-patient department of Neurology, All India Institute of Medical Sciences, New Delhi, India. Deoxyribonucleic acid for each case and control was isolated from peripheral blood using phenol-chloroform extraction method. Polymerase chain reaction-restriction fragment length polymorphism method was used to determine the polymorphism. Data were analyzed using STATA Software Version 13. Results: The mean age of IS patient was 52.8 ± 12.5 years and in control group was 50.97 ± 12.7 years. Genotypic frequency distributions were in accordance with Hardy–Weinberg equilibrium in both cases and controls. As expected hypertension, diabetes, dyslipidemia, smoking, heavy alcohol intake, family history of stroke, and poor economic status were significantly associated with the risk of IS. Multivariate analysis revealed 5.17 times higher odds for developing the risk of large vessel subtype of IS in patients carrying Factor V Leiden G1691A gene variation as compared to control subjects (OR, 5.17; 95% CI, 1.32–20.3, P = 0.01). Conclusion: The present study suggests that Factor V Leiden G1691A polymorphism may be significantly associated with the risk of large vessel subtype of IS. Large sample size studies using prospective cohort designs are required to corroborate the present findings. PMID:28904463

  12. Factor V Leiden, prothrombin G20210A, and methylene tetrahydrofolate reductase mutations and stillbirth: the Stillbirth Collaborative Research Network.

    Science.gov (United States)

    Silver, Robert M; Saade, George R; Thorsten, Vanessa; Parker, Corette B; Reddy, Uma M; Drews-Botsch, Carey; Conway, Deborah; Coustan, Donald; Dudley, Donald J; Bukowski, Radek; Rowland Hogue, Carol J; Pinar, Halit; Varner, Michael W; Goldenberg, Robert; Willinger, Marian

    2016-10-01

    An evaluation for heritable thrombophilias is recommended in the evaluation of stillbirth. However, the association between thrombophilias and stillbirth remains uncertain. We sought to assess the association between maternal and fetal/placental heritable thrombophilias and stillbirth in a population-based, case-control study in a geographically, racially, and ethnically diverse population. We conducted secondary analysis of data from the Stillbirth Collaborative Research Network, a population-based case-control study of stillbirth. Testing for factor V Leiden, prothrombin G20210A, methylene tetrahydrofolate reductase C677T and A1298C, and plasminogen activating inhibitor (PAI)-1 4G/5G mutations was done on maternal and fetal (or placental) DNA from singleton pregnancies. Data analyses were weighted for oversampling and other aspects of the design. Odds ratios (OR) were generated from univariate models regressing stillbirth/live birth status on each thrombophilia marker. Results were available for ≥1 marker in 488 stillbirths and 1342 live birth mothers and 405 stillbirths and 990 live birth fetuses. There was an increased odds of stillbirth for maternal homozygous factor V Leiden mutation (2/488; 0.4% vs 1/1380; 0.0046%; OR, 87.44; 95% confidence interval, 7.88-970.92). However, there were no significant differences in the odds of stillbirth for any other maternal thrombophilia, even after stratified analyses. Fetal 4G/4G PAI-1 (OR, 0.63; 95% confidence interval, 0.43-0.91) was associated with decreased odds of stillbirth. Other fetal thrombophilias were similar among groups. Most maternal and fetal thrombophilias were not associated with stillbirth. Maternal factor V Leiden was weakly associated with stillbirth, and the fetal PAI-1 4G/4G polymorphism was associated with live birth. Our data do not support routine testing for heritable thrombophilias as part of an evaluation for possible causes of stillbirth. Copyright © 2016. Published by Elsevier Inc.

  13. Portal vein thrombosis in a patient with HCV cirrhosis and combined hemophilia A and thrombophilia V Leiden

    Directory of Open Access Journals (Sweden)

    Nikos Eleftheriadis

    2010-10-01

    Full Text Available Nikos Eleftheriadis, Pantelis MakrisHemostatic Unit of The First Propedeutic Department of Internal Medicine, AHEPA Hospital, Aristotles University of Thessaloniki, GreeceAbstract: The relation of hemophilia A with thrombophilia V Leiden is extremely rare in the literature. Furthermore, hemophiliac patients have an increased risk of severe life-threatening hemorrhage, blood transfusions, and therefore hepatitis transmission, mainly hepatitis C (HCV.Aims and methods: We present a 54-year-old male with a 5-year history of decompensated liver cirrhosis on the grounds of HCV hepatitis, hemophilia A, and thrombophilia V Leiden. He was admitted to our department because of severe abdominal distension, resembling ‘tense ascites’ despite the use of diuretics. Clinical examination showed shifting dullness and a protuberant abdomen, while hematological and blood chemistry results revealed thrombopenia (platelets: 77000/mL and hypoalbuminemia. Repeated abdominal paracentesis (under factor VIII administration failed to remove ascitic fluid, while abdominal echosonography and computed tomography revealed severe edema of mesenterium and intraabdominal viscus and the absence of free ascitic fluid, atrophic cirrhotic liver, and splenomegaly. Moreover, abdominal doppler echosonography revealed signs of portal hypertension, previous portal vein thrombosis, and revascularization of the portal vein. Gastroscopy showed esophageal varices grade II, without signs of bleeding. A-FP and all other laboratory examinations were normal.Results: Our patient was intravenously treated with albumine and diuretics (furosemide with mild improvement of his abdominal distension. During his hospitalization he presented an episode of spontaneous bacterial peritonitis and hepatic encephalopathy, which were successfully treated with lactulose clysmas and ciprofloxacine. He was discharged in a good general condition.Conclusion: According to our case we consider the false clinical

  14. Prevalence of factor V G1691A (Leiden) and prothrombin G20210A polymorphisms among apparently healthy Jordanians.

    Science.gov (United States)

    Nusier, Mohamad K; Radaideh, Abdelrahman M; Ababneh, Nida'a A; Qaqish, Bara'ah M; Alzoubi, Renad; Khader, Yousef; Mersa, Janet Y; Irshaid, Nidal M; El-Khateeb, Mohammed

    2007-10-01

    Factor V Leiden and prothrombin G20210A are related genetic risk factors for venous thromboembolism (VTE). Analysis for both mutations is increasingly being performed on patients exhibiting hypercoagulability. The objective of this study was to determine the prevalence of factor V Leiden (FVL), prothrombin-G20210A (PT-G20210A) polymorphisms and their coexistence among apparently healthy Jordanians. One thousand apparently healthy individuals from representative regions of Jordan with no previous history of VTE participated in this study. The mean age of participants was 28.5+/-6.4 years (age range 18-45 years). Two hundred and eighteen subjects were APC resistant with an APC-R mean of 85.52+/-15.35 seconds; the non-resistant subjects had an APC-R mean of 159.90+/-26.96 seconds. A multiplex polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the simultaneous detection of FVL and prothrombin G20210A was used to analyze the 218 DNA samples that were APC-R resistant. Both mutations generate HindIII RFLPs and the prothrombin amplicon contains an invariant HindIII recognition sites. The multiplex PCR-RFLP of Factor V for those 218-samples was: 41 wild-type, 169 heterozygous mutant, and eight homozygous mutant individuals. For prothrombin G20210A, the multiplex PCR-RFLP identified 215 wild-type and three heterozygous mutant individuals. Factor V positive individuals (n=50) had a mean F-V activity of 78.04%+/-25.81. F-V activity among wild type (n=41), F-V Leiden heterozygous (n=169) and F-V Leiden homozygous (n=8) were 92.93%+/-16.17, 87.02%+/-15.21 and 96.14%+/-12.32, respectively. Factor II positive subjects (n=47) had a mean factor II activity of 127.96%+/-21.37. F-II activity among carriers (heterozygous, n=3) and non-carriers (normal, n=215) of PT-G20210A mutation were 107.67%+/-9.29 and 105.00%+/-17.79, respectively. The prevalence of FVL was 21.8% and there is a little likelihood of the co-inheritance of the FVL and PT-G20210A among

  15. Paget-Schroetter syndrome after a dental procedure in a patient with factor V Leiden (R506Q) heterozygosity.

    Science.gov (United States)

    Sharma, Prabin

    2017-04-01

    : Paget-Schroetter syndrome or effort thrombosis is characterized by spontaneous thrombosis of the upper extremity venous system, commonly seen in a young healthy patient after repetitive use of the upper extremities. It is rarely associated with coagulopathy and thus, hypercoagulable work-up is not usually a part of the investigation. We present a first case of a young woman, who was diagnosed with left upper extremity effort thrombosis following a dental procedure. Interestingly, she was also noted to be heterozygous for factor V Leiden mutation.

  16. SV-IV Peptide1–16 reduces coagulant power in normal Factor V and Factor V Leiden

    Directory of Open Access Journals (Sweden)

    Ferrazzi Paola

    2007-12-01

    Full Text Available Abstract Native Factor V is an anticoagulant, but when activated by thrombin, Factor X or platelet proteases, it becomes a procoagulant. Due to these double properties, Factor V plays a crucial role in the regulation of coagulation/anticoagulation balance. Factor V Leiden (FVL disorder may lead to thrombophilia. Whether a reduction in the activation of Factor V or Factor V Leiden may correct the disposition to thrombophilia is unknown. Therefore we tested SV-IV Peptide 1–16 (i.e. a peptide derived by seminal protein vescicle number IV, SV-IV to assess its capacity to inhibit the procoagulant activity of normal clotting factor V or Factor V Leiden (FVL. We found that SV-IV protein has potent anti-inflammatory and immunomodulatory properties and also exerts procoagulant activity. In the present work we show that the SV-IV Peptide 1–16, incubated with plasma containing normal Factor V or FVL plasma for 5 minutes reduces the procoagulant capacity of both substances. This is an anticoagulant effect whereas SV-IV protein is a procoagulant. This activity is effective both in terms of the coagulation tests, where coagulation times are increased, and in terms of biochemical tests conducted with purified molecules, where Factor X activation is reduced. Peptide 1–16 was, in the pure molecule system, first incubated for 5 minutes with purified Factor V then it was added to the mix of phosphatidylserine, Ca2+, Factor X and its chromogenic molecule Chromozym X. We observed a more than 50% reduction in lysis of chromogenic molecule Chromozym X by Factor Xa, compared to the sample without Peptide 1–16. Such reduction in Chromozym X lysis, is explained with the reduced activation of Factor X by partial inactivation of Factor V by Peptide 1–16. Thus our study demonstrates that Peptide 1–16 reduces the coagulation capacity of Factor V and Factor V Leiden in vitro, and, in turn, causes factor X reduced activation.

  17. Protein S testing in patients with protein S deficiency, factor V Leiden, and rivaroxaban by North American Specialized Coagulation Laboratories.

    Science.gov (United States)

    Smock, Kristi J; Plumhoff, Elizabeth A; Meijer, Piet; Hsu, Peihong; Zantek, Nicole D; Heikal, Nahla M; Van Cott, Elizabeth M

    2016-07-04

    In 2010-2012, the North American Specialized Coagulation Laboratory Association (NASCOLA) distributed 12 proficiency testing challenges to evaluate laboratory testing for protein S (PS). Results were analysed to assess the performance of PS activity, PS free antigen, and PS total antigen testing. Statistical analysis was performed on the numeric results and qualitative classification submitted for each method. There were 2,106 total results: 716 results from PS activity assays, 833 results from PS free antigen assays, and 557 results from PS total antigen assays. The three assay types performed well in the classification of five normal samples and nine abnormal samples, although certain PS activity methods were more likely to classify normal samples as abnormal and one PS total antigen assay was more likely to classify abnormal samples as normal. PS activity methods were affected by interfering substances such as heterozygous or homozygous factor V Leiden mutation (underestimation) and the anticoagulant drug rivaroxaban (overestimation). In conclusion, NASCOLA laboratories using a variety of PS assays performed well in the classification of clearly normal and abnormal samples. Laboratories performing PS activity assays should be aware of potential interferences in samples positive for FV Leiden or containing certain anticoagulant medications.

  18. Bilateral Legg-Calve-Perthes Disease and Kienbock's Disease in a Child With Factor V Leiden Thrombophilia: A Case Report.

    Science.gov (United States)

    Baltzer, Heather L; Riester, Scott; Moran, Steven L

    2016-09-01

    Background: The etiology of multifocal osteonecrosis is not definitively known; however, hypercoagulable state is a very plausible cause. Methods: We present an unusual case of a 12-year-old boy with a history of Legg-Calve-Perthes disease presenting with right wrist pain who was subsequently diagnosed with Kienbock's disease. The finding of multifocal osteonecrosis prompted testing for a hypercoagulable state that was positive for Factor V Leiden thrombophilia. A thorough literature review using Medline database was conducted to investigate associations between inherited hypercoagulable states and multifocal osteonecrosis. Results: Our literature review identified 2 similar cases of multifocal osteonecrosis associated with a hypercoagulable disorder in adult patients. There were no reports among the pediatric patient population. Meta-analysis has demonstrated a potential link between Legg-Calve-Perthes disease and Factor V Leiden thrombophilia. Conclusions: This study offers further evidence to support the theory that multifocal osteonecrosis may be linked to a hypercoagulable state. Patients presenting with multifocal osteonecrosis should undergo screening for hypercoagulable states. Further investigation is needed to ascertain the potential benefit of prophylactic anticoagulation in patients with a known hypercoagulable state and multifocal osteonecrosis.

  19. Pancreatic cancer-associated gene polymorphisms in a nation-wide cohort of p16-Leiden germline mutation carriers; A case-control study Medical Genetics

    NARCIS (Netherlands)

    T.P. Potjer (Thomas P.); N. van der Stoep (Nienke); J.J. Houwing-Duistermaat (Jeanine); I.C.A.W. Konings (Ingrid C.A.W.); C.M. Aalfs (Cora); P.C. van den Akker (Peter); M.G.E.M. Ausems (Margreet); C.J. Dommering (Charlotte); L. van der Kolk (Lizet); M.C. Maiburg (Merel C.); L. Spruijt (Liesbeth); A. Wagner (Anja); H. Vasen (Hans); F.J. Hes (Frederik)

    2015-01-01

    textabstractBackground: The p16-Leiden founder mutation in the CDKN2A gene is the most common cause of Familial Atypical Multiple Mole Melanoma (FAMMM) syndrome in the Netherlands. Individuals with this mutation are at increased risk for developing melanoma of the skin, as well as pancreatic cancer.

  20. Pancreatic cancer-associated gene polymorphisms in a nation-wide cohort of p16-Leiden germline mutation carriers; a case-control study

    NARCIS (Netherlands)

    T.P. Potjer (Thomas P.); N. van der Stoep (Nienke); J.J. Houwing-Duistermaat (Jeanine); I.C.A.W. Konings (Ingrid C.A.W.); C.M. Aalfs (Cora); P.C. van den Akker (Peter); M.G.E.M. Ausems (Margreet); C.J. Dommering (Charlotte); L. van der Kolk (Lizet); M.C. Maiburg (Merel C.); L. Spruijt (Liesbeth); A. Wagner (Anja); H. Vasen (Hans); F.J. Hes (Frederik)

    2015-01-01

    textabstractBackground: The p16-Leiden founder mutation in the CDKN2A gene is the most common cause of Familial Atypical Multiple Mole Melanoma (FAMMM) syndrome in the Netherlands. Individuals with this mutation are at increased risk for developing melanoma of the skin, as well as pancreatic cancer.

  1. China Back in the Frame. A comparative study of Canton, Whampoa and Macao harbour views in the Leiden Museum of Ethnology and in the Guangzhou Museum

    NARCIS (Netherlands)

    Poel, van der R.H.M.; Groenendijk, E.; Viallé, C.; Blussé, L.

    2009-01-01

    This article presents a brief overview of research results deriving from the investigation of a group of 18th and 19th century export oil paintings from China in the collection of the National Museum of Ethnology n Leiden. The oils were compared with a group of reverse glass paintings in the same co

  2. Risk of Recurrent Venous Thrombosis in Homozygous Carriers and Double Heterozygous Carriers of Factor V Leiden and Prothrombin G20210A

    NARCIS (Netherlands)

    Lijfering, Willem M.; Middeldorp, Saskia; Veeger, Nic J. G. M.; Hamulyak, Karly; Prins, Martin H.; Bueller, Harry R.; van der Meer, Jan

    2010-01-01

    Background-Homozygous or double heterozygous factor V Leiden and/or prothrombin G20210A is a rare inherited thrombophilic trait. Whether individuals with this genetic background have an increased risk of recurrent venous thrombosis is uncertain. Methods and Results-A case-control design within a lar

  3. Spix and Wagler type specimens of reptiles and amphibians in the Natural History Musea in Munich (Germany) and Leiden (The Netherlands)

    NARCIS (Netherlands)

    Hoogmoed, M.S.; Gruber, U.

    1983-01-01

    An evaluation of the existing SPIX/WAGLER type material in the museums in Munich and Leiden is given. It transpired that a considerable part of the type material, which was thought to have been destroyed during the second world war, is still extant. The material is described briefly, its presenttaxo

  4. Prevalence of coagulation factor II G20210A and factor V G1691A Leiden polymorphisms in Chechans, a genetically isolated population in Jordan.

    Science.gov (United States)

    Dajani, Rana; Fatahallah, Raja; Dajani, Abdelrahman; Al-Shboul, Mohammad; Khader, Yousef

    2012-09-01

    Coagulation factor II G20210A and coagulation factor V (Leiden) G1691A single nucleotide polymorphisms (SNPs) are major inherited risk factors of venous thromboembolism. In view of the heterogeneity in their world distribution and lack of sufficient information about their distribution among Chechans, we addressed the prevalence of these SNPs in the Chechan population in Jordan, a genetically isolated population. Factor II G20210A and factor V Leiden SNPs were analysed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method and Amplification refractory mutation detection system (ARMS) respectively in 120 random unrelated subjects from the Chechan population in Jordan. Among the subjects studied for factor II G20210A mutation there were three individuals carrying this mutation as heterozygous (one female and two male), giving a prevalence of 2.5 % and an allele frequency of 1.25 %. No homozygous factor II allele was found. Factor V Leiden G1691A mutation was detected as heterozygous in 22 of 120 of individuals (17 female and five male) indicating a prevalence of 18.3 % and allele frequency of 9.2 %. No homozygous allele was found. Our results indicated that prevalence of factor II G20210A mutation in the Chechan population is similar to prevalence in Jordan and Caucasian populations (1-6 %) while the prevalence of factor V Leiden was higher in the Chechan population compared to Jordan and Caucasian populations (2-15 %).

  5. A risk-benefit analysis of factor V Leiden testing to improve pregnancy outcomes: a case study of the capabilities of decision modeling in genomics.

    Science.gov (United States)

    Bajaj, Preeti S; Veenstra, David L

    2013-05-01

    We sought to assess the benefits, risks, and personal utility of factor V Leiden mutation testing to improve pregnancy outcomes and to assess the utility of decision-analytic modeling for complex outcomes in genomics. We developed a model to evaluate factor V Leiden testing among women with a history of recurrent pregnancy loss, including heparin therapy during pregnancy in mutation-positive women. Outcomes included venous thromboembolism, major bleeds, pregnancy loss, maternal mortality, and quality-adjusted life-years. Factor V Leiden testing in a hypothetical cohort of 10,000 women led to 7 fewer venous thromboembolic events, 90 fewer pregnancy losses, and an increase of 17 major bleeding events. Small improvements in quality-adjusted life-years were largely attributable to reduced mortality but also to improvements in health-related quality of life. However, sensitivity analyses indicate large variance in results due to data uncertainty. Furthermore, the complexity of outcomes limited our ability to fully capture the repercussions of testing in the quality-adjusted life-year measure. Factor V Leiden testing involves tradeoffs between clinical and personal utility, and additional effectiveness data are needed for heparin use to prevent pregnancy loss. Decision-analytic methods offer somewhat limited value in assessing these tradeoffs, suggesting that evaluation of complex outcomes will require novel approaches to appropriately capture patient-centered outcomes.Genet Med 2013:15(5):374-381.

  6. Do incident and recurrent venous thromboembolism risks truly differ between heterozygous and homozygous Factor V Leiden carriers? A retrospective cohort study.

    Science.gov (United States)

    Perez Botero, J; Ormsby, W D; Ashrani, A A; McBane, R D; Wysokinski, W E; Patnaik, M M; Lewis, B R; Grill, D E; Pruthi, R K; Heit, J A

    2016-05-01

    While Factor V Leiden (F5 rs6025 A allele) is a known venous thromboembolism (VTE) risk factor, VTE risk among heterozygous vs. homozygous carriers is uncertain. In a retrospective cohort study of Mayo Clinic patients referred for genotyping between 1996 and 2013, we tested Factor V Leiden genotype as a risk factor for incident and recurrent VTE. Among heterozygous (n=268) and homozygous (n=111) carriers, the prevalence of VTE was 54% and 68%, respectively (p=0.016). While mean patient age at first VTE event (43.9 vs. 42.9years; p=0.70) did not differ significantly, median VTE-free survival was modestly shorter for homozygous carriers (56.8 vs 59.5 years; p=0.04). Sixty-nine (48%) and 31 (42%) heterozygous and homozygous carriers had ≥1 VTE recurrence (p=0.42). In a multivariable model, idiopathic incident VTE and a second thrombophilia were associated with increased and anticoagulation duration >6months with reduced hazards of VTE recurrence; Factor V Leiden genotype was not an independent predictor of recurrence. Aside from a higher VTE prevalence and modestly reduced VTE-free survival, VTE penetrance and phenotype severity did not differ significantly among homozygous vs. heterozygous carriers, suggesting that VTE prophylaxis and management should not differ by Factor V Leiden genotype. Copyright © 2016 European Federation of Internal Medicine. Published by Elsevier B.V. All rights reserved.

  7. The Leiden/Argentine/Bonn (LAB) Survey of Galactic HI: Final data release of the combined LDS and IAR surveys with improved stray-radiation corrections

    NARCIS (Netherlands)

    Kaberla, P.M.W.; Burton, W.B.; Hartmann, L.; Arnal, E.M.; Bajaja, E.; Morras, R.; Pöppel, W.G.L.

    2005-01-01

    We present the final data release of observations of λ21-cm emission from Galactic neutral hydrogen over the entire sky, merging the Leiden/Dwingeloo Survey (LDS: Hartmann & Burton 1997, Atlas of Galactic Neutral Hydrogen) of the sky north of δ = −30◦ with the Instituto Argentino de Radioastronomía

  8. Borgen van verkeersveiligheid bij het aanbesteden van wegen : review van de aanbesteding van het RWS Zuid-Holland-project A4 Burgerveen-Leiden.

    NARCIS (Netherlands)

    Schermers, G. Wesemann, P. & Stipdonk, H.L.

    2008-01-01

    The Division Zuid-Holland of the Directorate-General for Public Works and Water Management has used a new procedure for contracting the widening of the A4 motorway near Leiden. The new procedure paid special attention to road safety in the framework of functional contracting. Tenderers could receive

  9. Association of resistance to activated protein with the presence of Leiden and Cambridge Factor V mutations in Mexican patients with primary thrombophilia.

    Science.gov (United States)

    Zavala Hernández, César; Hernández Zamora, Edgar; Martínez Murillo, Carlos; Arenas Sordo, María de la Luz; González Orozco, Ana Elena; Reyes Maldonado, Elba

    2010-01-01

    Leiden and Cambridge factor V coagulation mutations and activated protein C resistance (RaPC) are alterations related with vein and artery thrombosis. In this study we aimed to determine whether RaPC is associated with the presence of Leiden and Cambridge mutation and the frequency of these mutations in the racially mestizo Mexican population. We included 150 Mexican patients with primary thrombophilia and 100 healthy subjects in this study. RaPC was determined using commercial methods and genotypes FV Leiden and FV Cambridge with PCR-RFLPs. RaPC was positive in four patients and in one control individual; however, there was no presence of Leiden or Cambridge mutation in the studied group; thus, RaPC was not correlated with the presence of any of the studied mutations. These results indicate that there are other primary or secondary causes different from those studied, which condition the presence of RaPC. Furthermore, the frequency obtained for RaPC in our thrombophilic population of racially mixed Mexicans is lower compared to that obtained in the Caucasian population, most probably because they are genetically different populations.

  10. Factor V Leiden, factor V Cambridge, factor II GA20210, and methylenetetrahydrofolate reductase in cerebral venous and sinus thrombosis: A case-control study.

    Science.gov (United States)

    Saadatnia, Mohammad; Salehi, Mansour; Movahedian, Ahmad; Shariat, Seyed Ziaeddin Samsam; Salari, Mehri; Tajmirriahi, Marzieh; Asadimobarakeh, Elham; Salehi, Rasoul; Amini, Gilda; Ebrahimi, Homa; Kheradmand, Ehsan

    2015-06-01

    Factor V G1691A (FV Leiden), FII GA20210, and methylenetetrahydrofolate reductase (MTHFR) C677T mutations are the most common genetic risk factors for thromboembolism in the Western countries. However, there is rare data in Iran about cerebral venous and sinus thrombosis (CVST) patients. The aim of this study was to evaluate the frequency of common genetic thrombophilic factors in CVST patients. Forty consequently CVST patients from two University Hospital in Isfahan University of Medical Sciences aged more than 15 years from January 2009 to January 2011 were recruited. In parallel, 51 healthy subjects with the same age and race from similar population selected as controls. FV Leiden, FII GA20210, MTHFR C677T, and FV Cambridge gene mutations by polymerase chain reaction technique were evaluated in case and control groups. FV Leiden, FII GA20210, and FV Cambridge gene mutations had very low prevalence in both case (5%, 2%, 0%) and control (2.5%, 0%, 0%) and were not found any significant difference between groups. MTHFR C677T mutations was in 22 (55%) of patients in case group and 18 (35.5%) of control group (P = 0.09). This study showed that the prevalence of FV Leiden, FII GA20210, and FV Cambridge were low. Laboratory investigations of these mutations as a routine test for all patients with CVST may not be cost benefit.

  11. Risk of venous thromboembolism associated with single and combined effects of Factor V Leiden, Prothrombin 20210A and Methylenetethraydrofolate reductase C677T

    DEFF Research Database (Denmark)

    Simone, Benedetto; De Stefano, Valerio; Leoncini, Emanuele

    2013-01-01

    Genetic and environmental factors interact in determining the risk of venous thromboembolism (VTE). The risk associated with the polymorphic variants G1691A of factor V (Factor V Leiden, FVL), G20210A of prothrombin (PT20210A) and C677T of methylentetrahydrofolate reductase (C677T MTHFR) genes ha...

  12. List of the primary types of social wasps (Hymenoptera: Vespidae) deposited in the Nationaal Natuurhistorisch Museum, Leiden, and the Zoölogisch Museum, Amsterdam

    NARCIS (Netherlands)

    Kojima, J.; Achterberg, van C.

    1997-01-01

    The primary types of social wasp subfamilies Vespinae, Polistinae and Stenogastrinae housed in the Nationaal Natuurhistorisch Museum in Leiden and the Zoölogisch Museum in Amsterdam are listed. Their complete label data are given unless they are available elsewhere. Lectotypes of Polistes snelleni d

  13. Freqüência do fator V Leiden em indivíduos sob investigação de trombofilia, Recife, Pernambuco, Brasil Frequency of factor V Leiden in individuals under thrombophilia investigation, Recife, Pernambuco, Brazil

    Directory of Open Access Journals (Sweden)

    Catarina P. S. Ramos

    2006-06-01

    Full Text Available As tromboses são eventos de etiopatogênese multifatorial resultantes da interação de fatores genéticos e ambientais, constituindo na atualidade uma das causas mais comuns de morbimortalidade. Uma mutação de ponto no fator V da coagulação, o fator V Leiden (FVL, constitui o defeito genético mais comum associado com trombofilia. No Brasil, o estudo deste fator de risco é relativamente recente e se dispõe de poucos dados na literatura especializada. Este trabalho teve como objetivo determinar a freqüência da mutação do fator V Leiden em 292 indivíduos sob investigação de trombofilia no Hemocentro de Pernambuco. A técnica molecular utilizada foi a RE/PCR (Enzima de Restrição/Reação em Cadeia da Polimerase, usando primers específicos e a enzima MnlI. A freqüência do FVL encontrada foi de 13,3%, sendo 36 heterozigotos e 3 homozigotos. A presença da mutação foi semelhante em indivíduos com idade tanto inferior quanto superior a 45 anos. Os resultados da pesquisa mostraram que a freqüência do FVL na população estudada é semelhante à descrita na literatura científica para indivíduos selecionados com tromboembolismo e confirmam a importância do estudo molecular nas diferentes faixas etárias.Thrombosis is a multifactorial disease involving genetic and environmental factors and constitutes one of the most common causes of morbimortality. A point mutation in coagulation factor V - factor V Leiden (FVL, constitutes the most prevalent genetic defect associated with thrombophilias. The study of this risk factor is relatively recent in Brazil and only a few reports have been published to date. The aim of this study was to determine the frequency of FVL in 292 individuals being investigated for thrombophilia at the Pernambuco State Blood Center. The molecular biology technique used was RE/PCR (Restriction Enzyme / Polymerase Chain Reaction, using specific primers and the MnlI enzyme. The frequency of FVL was 13

  14. Cytomegalovirus-associated splenic infarcts in a female patient with Factor V Leiden mutation: a case report

    Directory of Open Access Journals (Sweden)

    Atzmony Lihi

    2008-12-01

    Full Text Available Abstract Introduction Cytomegalovirus-associated thrombosis has rarely been reported in the medical literature, and if so, mainly in immunocompromized patients. Case presentation We report the case of a 36-year-old Caucasian woman with acute cytomegalovirus infection presenting with spontaneous splenic infarcts. Trans-esophageal echocardiography did not show any vegetations or mural thrombi. The patient was also found to be heterozygous for the Factor V Leiden mutation. Anticoagulation treatment was considered but ruled out since cytomegalovirus was the obvious trigger for thrombosis in this patient. To the best of our knowledge, this is only the third report to date of cytomegalovirus-associated splenic infarcts. Conclusion This case report serves as additional evidence for the role of cytomegalovirus in thrombosis.

  15. Mesenteric vein thrombosis in a patient heterozygous for factor V Leiden and G20210A prothrombin genotypes.

    Science.gov (United States)

    Karmacharya, Paras; Aryal, Madan Raj; Donato, Anthony

    2013-11-21

    Mesenteric venous thrombosis (MVT) is a rare but life threatening form of bowel ischemia. It is implicated in 6%-9% of all cases of acute mesenteric ischemia. The proportion of patients with primary (or idiopathic) MVT varies from 0% to 49%, with a decrease in frequency secondary to more recent availability of newer investigations for hypercoagulability. The presence of factor V Leiden (FVL) and prothrombin G20210A mutations (PGM) have been well documented in these cases. However, there have been scarce case reports describing MVT in heterozygotes of both these mutations occurring simultaneously and its implications on long term management. Our case describes acute MVT in a previously asymptomatic young patient with no prior history of venous thromboembolism. The patient was found to be heterozygous for FVL and PGM and treated with lifelong anticoagulation with warfarin (goal international normalized ratio: 2-3) and avoidance of hormonal contraceptives.

  16. [Neonatal renal vein thrombosis in a heterozygous carrier of both factor V Leiden and the MTHFR gene mutation].

    Science.gov (United States)

    Wannes, S; Soua, H; Ghanmi, S; Braham, H; Hassine, M; Hamza, H A; Ben Hamouda, H; Sfar, M-T

    2012-04-01

    Renal vein thrombosis (RVT) is a rare but potentially serious neonatal disease. Its epidemiology and its clinical and biological expression are currently well known, but its etiological exploration, like that of venous thromboembolism, is increasingly complex. Perinatal risk factors such as prematurity, dehydration, and birth asphyxia have lost their direct accountability at the expense of their interaction with constitutional disorders of hemostasis. We report a case of RVT in a newborn who was a heterozygous carrier of both factor V Leiden and the methylene tetrahydrofolate reductase (MTHFR) gene mutation. We recall the clinical and epidemiological characteristics. A search for inborn blood coagulation disorders should be systematic in the newborn infant with venous thrombosis because of the risk of recurrence, taking into account perinatal factors and maternal thrombophilia (especially if RVT is established during the prenatal period).

  17. Activated Protein C-Resistance Determination and Vascular Access Thrombosis in Populations with High Prevalence of Factor V Leiden.

    Science.gov (United States)

    Androulakis, Nikolaos E; Tzenakis, Nikolaos; Nioti, Eleni; Spatharaki, Paraskevi; Vyzoukaki, Rodanthi; Papadopoulou, Anastasia; Kokonozaki, Maria; Alexandrakis, Michael G

    2015-01-01

    Factor V Leiden heterozygosity occurs in 3-8% of the general European and US populations. Activated protein C resistance (APC-R)--a non-molecular laboratory test--can efficiently demonstrate the presence of this mutation and can be performed on most coagulation analyzers. On the other hand, fistula or graft thrombosis is a common and costly complication in hemodialysis patients. Our aim was to establish the value of APC-R determination in hemodialysis patients by assessing the risk of access thrombosis in patients with increased APC-R. A total of 133 patients (81 men, mean age 64.5 ± 14.9 years and 52 women, mean age 63.6 ± 15 years) were selected. Participants were divided into 2 groups: those with access thrombosis (54 patients, 40.6%) and those with no access thrombosis (79 patients, 59.4%), and they were tested for the most common congenital or acquired thrombophilia risk factors. Overall, 12 patients (9%) had an increased APC-R and 10 of them had at least 1 episode of access thrombosis (83.3%). Univariate analysis to estimate crude odds ratio (OR) showed an OR of 8.8 (95% CI 1.8-41.8) times higher risk for access thrombosis in these patients. No significant differences were found after adjusting for age, hypertension, diabetes mellitus, coronary artery disease, cerebrovascular disease, peripheral arterial disease and malignancy. Sex was also a factor influencing thrombosis, presenting a higher OR for women (OR 2.2, 95% CI 1.1-4.4). This study revealed a significant association between access thrombosis and increased APC-R in hemodialysis patients. This indicates that the determination of APC-R should be considered--especially, in populations with a high prevalence of Factor V Leiden--as proper anticoagulant therapy in these patients may reduce the risk of access thrombosis. © 2015 S. Karger AG, Basel.

  18. Clinical significance of factor V leiden and prothrombin G20210A-mutations in cerebral venous thrombosis - comparison with arterial ischemic stroke.

    Science.gov (United States)

    Beye, Aida; Pindur, Gerhard

    2017-08-28

    Cerebrovascular diseases are considered in a different way concerning their etiology with regard to arterial and venous occlusion. The role of thrombophilia in this context remains undetermined. For this reason, a case-control study was conducted including a total of 202 patients (154 females, 48 males) aged from 18 to 76 years (mean: 39.8 years) suffering either from cerebral sinus venous thrombosis (n = 101) or from arterial ischemic stroke (n = 101). Study groups were evaluated on the basis of age- and gender-matched pairs. Gene mutations of factor V-1691 (factor V Leiden) and prothrombin-20210 being considered as the most common thrombophilia markers were analyzed in this study. Factor V Leiden-mutations were found in 16.8% of patients with cerebral sinus venous thrombosis (CVT) and in 17.8% of patients with arterial ischemic stroke (AIS), which was significantly more frequent than in controls at a rate of 4.95% (ORs: 3.89 and 4.16). Prothrombin-mutations were significantly more frequent in CVT at a rate of 14.9% versus 2.97% in controls (OR: 5.70). This does not apply for AIS showing a rate of 4.95% prothrombin-mutations. Rates of factor V Leiden-mutations are not different in CVT compared with AIS. In contrast, however, prothrombin-mutations were significantly more frequent in CVT than in AIS with a rate of 14.9% versus 4.95% (OR 3.35). Furthermore, 3 cases with combined heterozygosity of factor V Leiden- and prothrombin-mutation have been identified in CVT, but not in AIS or controls. All of the above mentioned mutations were exclusively heterozygous. We conclude from these data that thrombophilia in terms of factor V Leiden genotype is a risk factor for both CVT and AIS in equal measure. In contrast, prothrombin-20210-mutations were different playing a significant role in the pathogenesis of cerebral sinus vein- thrombosis, but not in arterial ischemic stroke. Also, the combined occurrence of heterozygous prothrombin- and factor V Leiden

  19. Association analysis of insulin-like growth factor-1 axis parameters with survival and functional status in nonagenarians of the Leiden Longevity Study

    DEFF Research Database (Denmark)

    van der Spoel, Evie; Rozing, Maarten P; Houwing-Duistermaat, Jeanine J

    2015-01-01

    with old age survival and functional status in nonagenarians from the Leiden Longevity Study. This study examined 858 Dutch nonagenarian (males≥89 years; females≥91 years) siblings from 409 families, without selection on health or demographic characteristics. Nonagenarians were divided over sex.......91) compared to the quartile with the highest ratio (ptrend=0.002). Functional status was assessed by (Instrumental) Activities of Daily Living ((I)ADL) scales. Compared to those in the quartile with the highest IGF-1/IGFBP3 ratio, nonagenarians in the lowest quartile had higher scores for ADL (ptrend=0.......001) and IADL (ptrend=0.003). These findings suggest that IGF-1 axis parameters are associated with increased old age survival and better functional status in nonagenarians from the Leiden Longevity Study....

  20. Heerlen polymorphism associated with type III protein S deficiency and factor V Leiden mutation in a Polish patient with deep vein thrombosis.

    Science.gov (United States)

    Wypasek, Ewa; Potaczek, Daniel P; Alhenc-Gelas, Martine; Undas, Anetta

    2014-01-01

    Protein S is one of the major natural anticoagulants. A missense serine 501 to proline (S501P) Heerlen polymorphism is associated with reduced levels of free protein S. Heerlen polymorphism, especially when combined with other thrombophilia risk factors, can lead to thromboembolic complications. To our knowledge, we report here the first Polish case associated with heterozygous Heerlen polymorphism resulting in type III protein S deficiency, detected in a 50-year-old man with several thrombotic episodes of deep and superficial veins and a highly positive thrombotic family history. The patient also had factor V Leiden mutation and persistently elevated anticardiolipin antibodies. It seems that increased risk of thrombotic complications could be explained in the patient by a synergy between the effects of Heerlen polymorphism, factor V Leiden heterozygous status and antiphospholipid syndrome.

  1. A Right Middle Cerebral Artery Infarct After Frontal Eosinophilic Granuloma Resection in an 8-Year-Old Boy with Factor V Leiden.

    Science.gov (United States)

    Cakir, Ertugrul; Arslan, Erhan; Yazar, Ugur; Reis, Gokce Pinar

    2015-01-01

    Stroke in children is relatively uncommon. We describe an 8-year-old boy diagnosed with primary eosinophilic granuloma (EG) of the frontal bone. After excision of the EG, the postoperative course was eventful. The patient had an acute right middle cerebral artery (MCA) infarct and had been comatose with a diminished Glasgow Coma Scale (GCS) score of 5. Urgent decompressive hemicraniectomy with duraplasty was performed. The postoperative course after the second operation was uneventful. Hematological tests revealed a diagnosis of factor V Leiden. The patient was discharged with left hemiparesis and GCS of 15. To the best of our knowledge, no such clinical picture of MCA infarction after EG excision has been described before. Neurosurgeons should be aware of inherited thrombophilias, such as factor V Leiden, if the postoperative clinical course worsens because of cerebral artery thrombosis. Also, decompressive hemicraniectomy could be life saving and should be performed urgently without any hesitation.

  2. Combined heterozygosity of factor V leiden and the G20210A prothrombin gene mutation in a patient with cerebral cortical vein thrombosis.

    Science.gov (United States)

    Liu, X Y; Gabig, T G; Bang, N U

    2000-07-01

    Cerebral venous thrombosis (CVT) is a rare type of stroke with a variety of causes. Several reports have suggested that either factor V Leiden or G20210A prothrombin gene mutation is associated with an increased risk of CVT. The genetic thrombophilias are typically associated with other predisposing factors. We report a unique case of CVT in a patient with both the factor V Leiden and the G20210A prothrombin gene mutations without other identifiable precipitating factors in a 28-year-old white male in good health. MRI and cerebral arterial angiography showed cerebral cortical venous thrombosis. This case suggests that combined heterozygous individuals may be particularly prone to spontaneous thrombosis, like CVT.

  3. Deep vein thrombosis, ecythyma gangrenosum and heparin-induced thrombocytopenia occurring in a man with a heterozygous Factor V Leiden mutation

    OpenAIRE

    Mariya Apostolova; Baoying Weng; Pote, Harry H.; Harold Ashcraft; Curtis Goldblatt; Paul V. Woolley

    2012-01-01

    Skin necrosis and limb gangrene are occasional thrombotic manifestations of anticoagulation therapy. We report a man heterozygous for the Factor V Leiden (FVL) mutation, and with a history of recurrent deep venous thrombosis, who initially presented with a necrotic skin lesion of the right flank while on warfarin therapy with a therapeutic international normalized ratio. Warfarin was discontinued and he received intravenous heparin. Thereafter he developed thrombocytopenia and pedal erythema ...

  4. The relationship between factor V Leiden, prothrombin G20210A, and MTHFR mutations and the first major thrombotic episode in polycythemia vera and essential thrombocythemia.

    Science.gov (United States)

    Trifa, Adrian P; Cucuianu, Andrei; Popp, Radu A; Coadă, Camelia A; Costache, Roxana M; Militaru, Mariela S; Vesa, Ştefan C; Pop, Ioan V

    2014-02-01

    Arterial and venous thrombosis are the most frequent complications in patients with polycythemia vera and essential thrombocythemia. We sought to demonstrate a possible contribution of the factor V Leiden, prothrombin G20210A, and methylenetetrahydrofolate reductase (MTHFR) 677 C > T and 1298 A > C mutations to the thrombotic risk in patients with polycythemia vera and essential thrombocythemia along with other biological features of these patients. We included 86 patients with polycythemia vera, of which 34 (39.5 %) had major thrombosis and 95 patients with essential thrombocythemia, of which 22 (23.1 %) had major thrombosis. In the whole cohort of patients, only the factor V Leiden mutation was significantly associated with both arterial and venous thrombosis in univariate and multivariate analysis (odds ratio (OR) = 4.3; 95 % confidence interval (CI) = 1.5-12.5; p = 0.008 and OR = 4.3; 95 % CI = 1.2-15.9; p = 0.02, respectively). Other factors significantly associated with thrombosis in both univariate and multivariate analysis were male sex (OR = 2.8, 95 % CI = 1.4-5.4, p = 0.002 and OR = 3.5, 95 % CI = 1.6-7.6, p = 0.002, respectively) and the JAK2 V617F mutation (OR = 5.5, 95 % CI = 2.1-15, p = 0.0001 and OR = 6.9, 95 % CI = 2.2-21.2, p = 0.001, respectively). In conclusion, among the four mutations analyzed (factor V Leiden, prothrombin G20210A, and MTHFR 677 C > T and 1298 A > C), only factor V Leiden is a major contributor to thrombosis in polycythemia vera and essential thrombocythemia.

  5. Contribution à l'étude des Hepialidae (22ème note) : Hepialidae du Musée de Leiden

    NARCIS (Netherlands)

    Viette, P.E.L.

    1950-01-01

    On trouvera ici l'étude d'une partie des Hepialidae du Musée de Leiden que le Dr. van Regteren Altena a bien voulu nous confier pour étude, ce dont le remercions vivement. Nous y avons ajouté l'étude de quatre espèces du British Museum (N.H.) et une du Museum de Paris. HÉPIALIDES DE LA NOUVELLE GUIN

  6. Macht, machinaties en musea. Jan van der Hoeven, Hermann Schlegel en hun strijd om het Rijksmuseum van natuurlijke historie te Leiden

    Directory of Open Access Journals (Sweden)

    H.L. de Jonge

    2005-01-01

    Full Text Available Might, machinations and museums. Jan van der Hoeven, Hermann Schlegel and their battle over the National Museum for Natural History in LeidenMuseums had a central place in nineteenth-century natural history. Aside from being important places for carrying out research into nature, they were also powerful vehicles for transmitting and communicating scientific knowledge. That is why, in the nineteenth century, natural history museums were frequently battlegrounds for rival scientists. Often, clashes over scientific matters were at the same time conflicts about power and space. After all, the naturalist who could get control over a museum’s collection and decide how the specimens should be classified and exhibited ultimately had the power to dictate which scientific knowledge was communicated; which story was told by a collection of specimens. This article is about the power struggle over the directorate of the National Museum of Natural History in Leiden that broke out between Jan van der Hoeven, Professor of Natural History and Comparative Anatomy at Leiden and Herman Schlegel, the museum’s chief curator. Both were rooted in very different natural historical traditions and had completely different views on the role of the museum and how the collection should be arranged and exhibited. In this article, we follow the two scientists in their attempts to gain support for their nominations. In this way, we also get a picture of nineteenth century Dutch political culture and the role scientists could play in it.

  7. Lack of association between factor V Leiden and prothrombin G20210A polymorphisms in Tunisian subjects with a history of myocardial infarction.

    Science.gov (United States)

    Berredjeb Ben Slama, Dhouha; Fekih-Mrissa, Najiba; Haggui, Abdeddayem; Nsiri, Brahim; Baraket, Nadia; Haouala, Habib; Gritli, Nasreddine

    2013-01-01

    Myocardial infarction is a multifactorial disease. It is provoked by occlusions in the coronary arteries resulting from exposure to multiple risk factors. To study the risk of myocardial infarction associated with the gene polymorphisms of factor V Leiden and factor II (G20210A). Cases consisted of 100 myocardial infarction patients who were hospitalized in the Principal Military Hospital of Tunis and 200 control subjects with no history of myocardial infarction. The prevalence of the factor V Leiden was higher in myocardial infarction patients (9%) than in control subjects (6%) with an OR=1.55 (95% CI=0.58-4.12), whereas the prevalence of prothrombin G20210A mutation was 3% and 2.5% in the patient and control groups, respectively [OR=1.21 (95% CI=0.22-5.94)]. Our results indicate that neither factor V Leiden nor the prothrombin G20210A contributed to the risk factors for myocardial infarction. Copyright © 2013 Elsevier Inc. All rights reserved.

  8. Association between the V Leiden G1691A mutation and sudden sensorineural hearing loss in Italian population: a meta-analysis.

    Science.gov (United States)

    Shu, Jingcheng; Si, Yongfeng; Yin, Shihua; He, Meirong

    2016-09-01

    Epidemiological studies have reported inconsistent findings on the association between the V Leiden G1691A mutation and sudden sensorineural hearing loss (SSNHL) in Italian population. The aim of this meta-analysis was to clarify this association. PubMed, Embase, and the China National Knowledge Infrastructure (CNKI) were searched up to April 1, 2015. We used STATA12.0 to calculate summary odds ratios (ORs) with 95 % confidence intervals (CIs). Four studies including 958 patients were identified. Pooled data showed no significant association between V Leiden G1691A mutation and risk of SSNHL in Italian population: A vs. G (OR = 1.660, 95 % CI 0.428-6.446, P OR = 0.464) and AG vs. GG (OR = 1.680, 95 % CI 0.422-6.688, P OR = 0.462). The present meta-analysis suggests that V Leiden G1691A mutation is not significantly associated with increased risk of SSNHL disease in Italian population. Further large and well-designed studies are needed to confirm this association.

  9. [The 1691 G > A (factor V Leiden) and 1328 T > C V coagulation factor polymorphisms and recurrent miscarriages].

    Science.gov (United States)

    Bałajewicz-Nowak, Marta; Pityński, Kazimierz; Milewicz, Tomasz

    2015-01-01

    Objectives: Inherited thrombophilia might lead to recurrent pregnancy loss (RPL). The aim of the study was to estimate the prevalence of V coagulation factor polymorphisms related with inherited thrombophilia among women in Malopolska region.Material and methods: Group of 136 women, who experienced at least 2 unexplained, idiopathic pregnancy loss. 106 healthy women having at least one uncomplicated pregnancy and delivered healthy children constituted a control group. Each patient were examined for factor V Leiden (FVL) and mutation 1328 T>C of factor V gene with use of real –time PCR and Taq-Man probes.Results: Among patients with RPL inhabiting region of Malopolska compared to control group occurred higher prevalence of FVL and mutation 1328 T>C. There is coincidence of polymorphism 1328 T>C of factor V gene and FVL in group of early and late RPL.Conclusions: TC genotype of 1328 T>C mutation carriers reveal tendency toward RPL below 7 weeks of pregnancy.Based on results of these findings inherited thrombophilia evaluation in patients after two or more RPL should be recommended.

  10. Sudden hearing loss followed by deep vein thrombosis and pulmonary embolism in a patient with factor V Leiden mutation.

    Science.gov (United States)

    Lovato, A; Tormene, D; Staffieri, C; Breda, S; Staffieri, A; Marioni, G

    2014-09-01

    Factor V Leiden (FVL) is by far the most prevalent inherited thrombophilic abnormality in Western countries, and this genetic condition has been associated with sudden sensorineural hearing loss (SSHL). Audiologists should be aware that SSHL may be the precursor of life-threatening thromboembolic events, especially in Caucasians who are more likely to be FVL carriers. Case report. A 41-year-old male patient. Although this is not the first report of SSHL in a FVL carrier, it is the first to describe SSHL occurring in a heterozygous FVL carrier who--within a month--was also diagnosed with deep vein thrombosis of the left common femoral, saphenous, and popliteal veins, and pulmonary embolism of the left pulmonary artery branch serving the posterior basal segment of the inferior lobe. SSHL is an emergency condition that warrants prompt medical examination and treatment. Hematological investigations should be considered in SSHL patients at least for those with a family history of thrombotic events, and for women on estrogen-progestin therapy or during pregnancy, with a view to providing adequate antithrombotic prophylaxis and reducing the risk of other thromboembolic events.

  11. Hepatocyte-specific IKK-β activation enhances VLDL-triglyceride production in APOE*3-Leiden mice.

    Science.gov (United States)

    van Diepen, Janna A; Wong, Man C; Guigas, Bruno; Bos, Jasper; Stienstra, Rinke; Hodson, Leanne; Shoelson, Steven E; Berbée, Jimmy F P; Rensen, Patrick C N; Romijn, Johannes A; Havekes, Louis M; Voshol, Peter J

    2011-05-01

    Low-grade inflammation in different tissues, including activation of the nuclear factor κB pathway in liver, is involved in metabolic disorders such as type 2 diabetes and cardiovascular diseases (CVDs). In this study, we investigated the relation between chronic hepatocyte-specific overexpression of IkB kinase (IKK)-β and hypertriglyceridemia, an important risk factor for CVD, by evaluating whether activation of IKK-β only in the hepatocyte affects VLDL-triglyceride (TG) metabolism directly. Transgenic overexpression of constitutively active human IKK-β specifically in hepatocytes of hyperlipidemic APOE*3-Leiden mice clearly induced hypertriglyceridemia. Mechanistic in vivo studies revealed that the hypertriglyceridemia was caused by increased hepatic VLDL-TG production rather than a change in plasma VLDL-TG clearance. Studies in primary hepatocytes showed that IKK-β overexpression also enhances TG secretion in vitro, indicating a direct relation between IKK-β activation and TG production within the hepatocyte. Hepatic lipid analysis and hepatic gene expression analysis of pathways involved in lipid metabolism suggested that hepatocyte-specific IKK-β overexpression increases VLDL production not by increased steatosis or decreased FA oxidation, but most likely by carbohydrate-responsive element binding protein-mediated upregulation of Fas expression. These findings implicate that specific activation of inflammatory pathways exclusively within hepatocytes induces hypertriglyceridemia. Furthermore, we identify the hepatocytic IKK-β pathway as a possible target to treat hypertriglyceridemia.

  12. Polymorphisms at activated protein C cleavage sites of factor V: Are they important in the absence of factor V Leiden?

    Directory of Open Access Journals (Sweden)

    Ehsan Kheradmand

    2017-01-01

    Full Text Available Background: Activated protein C (APC inactivates factor V (FV by cleavage of its heavy chain at Arg306, Arg506, Arg679, and Lys994. Mutational changes, which abolish APC cleavage sites, may predispose thrombosis by altering the inactivation process of FV. FV Leiden (FVL (Arg506Glu has been demonstrated as a strong risk factor for thrombosis. In the current study, we have studied whether mutations in the cleavage sites of FV for APC, not due to FVL, would have a role in presenting APC resistance (APCR and initiation of a cerebral thrombotic event.Methods: A group of 22 patients with a history of cerebral venous thrombosis (CVT, who were not carriers of FVL enrolled in the study. The patients who had conditions associated with acquired APCR were excluded from the study. APCR test was performed on the remaining 16 patients, which showed APCR in 4 plasma samples. DNA sequencing was performed on four exons of FV of APCR patients, encoding Arg306, Arg506, Arg679, and Lys994.Results: Mutations were not found within nucleotides encoding the cleavage sites; neither was found within their close upstream and downstream sequences.Conclusion: Our results show that polymorphisms affecting cleavage sites of FV other than Arg506Glu it would be less likely to be the basis for APCR and its increased thrombosis susceptibility. In addition, it emphasizes on the importance of screening for APCR in the patients diagnosed with CVT.

  13. Successful Living-Related Renal Allograft in a Recipient With Factor V Leiden Deficiency: A Case Report.

    Science.gov (United States)

    Florou, Evangelia; Koukoulaki, Maria; Theodoros, Theodoridis; Kalatzis, Vasileios; Vougas, Vasileios; Stamataki, Elissavet; Kokkinou, Vasiliki Christopoulou; Kostakis, Alkiviadis; Drakopoulos, Spiros

    2017-02-01

    Thrombophilia due to activated protein C resistance (Leiden mutation) is the most common inherited thrombophilic disorder with 5% incidence in whites. Renal transplant of these patients entails a risk of vascular thrombosis soon after the transplant; and acute rejection episodes and graft loss within the first year. We present a case of a successful living-related renal transplant in man with a recent history of repeat episodes of vascular access thrombosis attributed to inherited thrombophilia (heterozygosity for factor V mutation Q506 and homozygosity for mutation T677 for methylene-tetrahydrofolate reductase). Transplant recipient was administered anticoagulation therapy with low molecular weight heparin pre- and postoperatively. No thrombotic or hemorrhagic events occurred posttransplant. A high suspicion of thrombophilic disorders in patients with end-stage renal disease with vascular access thrombotic events should be screened further to prevent failure of a subsequent renal transplant. Inherited thrombophilic disorders may not exclude living-related kidney transplant provided that anticoagulation therapy is admin-istered perioperatively.

  14. Protective effect of compression socks in a marathon runner with a genetic predisposition to thrombophilia due to Factor V Leiden.

    Science.gov (United States)

    Zaleski, Amanda L; Pescatello, Linda S; Thompson, Paul D; Taylor, Beth A

    2015-07-01

    The present case study is an analysis of the effect of compression socks on hemostatic activation following a marathon in a female endurance athlete found to be heterozygous for the coagulation factor V (F5 1691 G>A [Arg>Gln rs6025/560]) risk allele that predisposes one to a genetically inherited disorder of blood clotting, Factor V Leiden. Markers for coagulation and fibrinolysis were obtained 24 h prior to (PRE), immediately after (FINISH) and 24 h after (POST) completion of two marathons: the first in which the runner was not wearing compression socks, and the second in which the runner wore compression socks throughout the race. Compression socks worn during a marathon appeared to lower the overall impact on hemostasis as well as clot formation in this particular athlete as evidenced by lower t-PA (-56%), TAT (-63%) and D-dimer (-30%). Hemostatic activation may be lower with the use of compression socks, and thus may be effective for preserving hemostasis in endurance athletes at risk.

  15. Low blood pressure predicts increased mortality in very old age even without heart failure: the Leiden 85-plus Study.

    Science.gov (United States)

    Poortvliet, Rosalinde K E; Blom, Jeanet W; de Craen, Anton J M; Mooijaart, Simon P; Westendorp, Rudi G J; Assendelft, Willem J J; Gussekloo, Jacobijn; de Ruijter, Wouter

    2013-05-01

    To investigate whether low systolic blood pressure is predictive for increased mortality risk in 90-year-old subjects without heart failure, defined by low levels of NT-proBNP, as well as in 90-year-old subjects with high levels of NT-proBNP. This study was embedded in the Leiden 85-plus Study, an observational population-based prospective study. All 90-year-old participants (n = 267) were included between 2002 and 2004 and followed up for mortality for at least 5 years. Differences in mortality risks were compared between participants with low systolic blood pressure (≤150 mmHg) and high systolic blood pressure (>150 mmHg) within strata of low NT-proBNP (low NT-proBNP, low systolic blood pressure gave a two-fold increased risk (hazard ratio 2.0, 95% confidence interval 1.1-3.4) compared with participants with high systolic blood pressure. For participants with high NT-proBNP, low systolic blood pressure provided a 1.7 increased mortality risk (95% confidence interval 1.2-2.3) compared with high systolic blood pressure. Low systolic blood pressure is predictive for increased mortality risk in 90-year-old subjects, irrespective of the NT-proBNP level. Therefore, the absence or presence of heart failure as determined by NT-proBNP does not influence the prognostic value of low systolic blood pressure with regard to mortality in the oldest old.

  16. High blood pressure and resilience to physical and cognitive decline in the oldest old: the Leiden 85-plus Study.

    Science.gov (United States)

    Sabayan, Behnam; Oleksik, Anna M; Maier, Andrea B; van Buchem, Mark A; Poortvliet, Rosalinde K E; de Ruijter, Wouter; Gussekloo, Jacobijn; de Craen, Anton J M; Westendorp, Rudi G J

    2012-11-01

    To evaluate the association between various blood pressure (BP) measures at age 85 and future decline in physical and cognitive function the oldest old. Longitudinal study. The population-based Leiden 85-plus Study. Five hundred seventy-two 85-year-old community-dwelling individuals. BP was measured at age 85 during home visits. Activities of daily living (ADLs) and Mini-Mental State Examination (MMSE) were assessed at age 85 and annually thereafter up to age 90. On average, participants were followed for 3.2 years. Cross-sectional and longitudinal analyses were performed using linear regression models using systolic BP (SBP), diastolic BP (DBP), mean arterial pressure (MAP), and pulse pressure (PP) as the determinants. All analyses were adjusted for sociodemographic and cardiovascular factors. At age 85, higher SBP and PP were associated with lower ADL disability scores (both P = .01). Similarly, higher SBP, DBP, and MAP were associated with higher MMSE scores (all P physical and cognitive decline, especially in individuals with pre-existing physical disability. © 2012, Copyright the Authors Journal compilation © 2012, The American Geriatrics Society.

  17. Effect of dabigatran on a prothrombinase-based assay for detecting activated protein C resistance: an ex vivo and in vitro study in normal subjects and factor V Leiden carriers.

    Science.gov (United States)

    Gessoni, Gianluca; Valverde, Sara; Valle, Letizia; Caruso, Pierpaolo; Gessoni, Francesca; Valle, Roberto

    2017-03-07

    The aim of this study was to evaluate ex vivo and in vitro interference of a direct factor IIa inhibitor, dabigatran, on a prothrombinase-based assay to detect activated protein C resistance. An ex vivo study was performed in six heterozygous factor V Leiden carriers and 12 normal subjects without the factor V Leiden mutation who were treated with dabigatran. An in vitro study was also performed considering 12 plasma samples (six from normal subjects and six from heterozygous factor V Leiden carriers) spiked with dabigatran. The dabigatran concentration was evaluated using a diluted thrombin time assay, activated protein C resistance was evaluated using a prothrombinase-based assay. In both the ex vivo and in vitro studies dabigatran interfered significantly with activated protein C resistance ratios observed in normal subjects and in factor V Leiden heterozygous carriers. The results reported in this paper seem to confirm that dabigatran is able to interfere with the Penthafarm prothrombinase-based assay used to study activated protein C resistance, significantly increasing observed ratios. This effect appears to be present already at low concentrations of dabigatran (6 ng/mL) and affects both normal subjects and heterozygous carriers of factor V Leiden. In this group of patients, dabigatran, at concentrations in the therapeutic range (100-200 ng/mL), could markedly increase the activated protein C resistance ratio, bringing it up to within the reference range for normal subjects, thus potentially leading to misclassification of patients.

  18. Free Form Technology from Delft

    NARCIS (Netherlands)

    Eekhout, A.J.C.M.; Van Gelder, B.L.; Lockefeer, W.A.A.M.; Veltkamp, M.; Vollers, K.J.

    2016-01-01

    The success of the Guggenheim Museum in Bilbao, designed and engineered by Frank O. Gehry and inaugurated in 1997, opened the eyes of the world to the plastic possibilities of Free Form Design. That is, on the side of architects and their admiring clients. Some architects draw up complicated but sur

  19. Delft Aerospace engineering integrated curriculum

    NARCIS (Netherlands)

    Kamp, A.

    2011-01-01

    The complex multidisciplinary problems and challenges in our society require deep problem solvers in science, management and engineering who are also capable of interacting with and understanding specialists from a wide range of disciplines and functional areas. Industry refers to these people as T-

  20. TU Delft en online media

    NARCIS (Netherlands)

    Speekenbrink, R.J.H.

    2013-01-01

    Dit boek behandelt een visie op online media bij Nederlandse universiteiten. Daarbij beperk ik me tot die aspecten waarin online communicatie en/of online marketing een rol speelt. Dit boek behandelt niet de onderwijsgerelateerde aspecten van online media. Elektronische leeromgevingen, sociale medi

  1. TU Delft en online media

    NARCIS (Netherlands)

    Speekenbrink, R.J.H.

    2013-01-01

    Dit boek behandelt een visie op online media bij Nederlandse universiteiten. Daarbij beperk ik me tot die aspecten waarin online communicatie en/of online marketing een rol speelt. Dit boek behandelt niet de onderwijsgerelateerde aspecten van online media. Elektronische leeromgevingen, sociale medi

  2. TU Delft en online media

    NARCIS (Netherlands)

    Speekenbrink, R.J.H.

    2013-01-01

    Dit boek behandelt een visie op online media bij Nederlandse universiteiten. Daarbij beperk ik me tot die aspecten waarin online communicatie en/of online marketing een rol speelt. Dit boek behandelt niet de onderwijsgerelateerde aspecten van online media. Elektronische leeromgevingen, sociale

  3. Fetal and neonatal exposure to trans-fatty acids impacts on susceptibility to atherosclerosis in apo E*3 Leiden mice.

    Science.gov (United States)

    Gates, Louise; Langley-Evans, Simon C; Kraft, Jana; Lock, Adam L; Salter, Andrew M

    2017-02-01

    Nutrition during pregnancy can impact on the susceptibility of the offspring to CVD. Postnatal consumption of trans-fatty acids (TFA), associated with partially hydrogenated vegetable oil (PHVO), increases the risk of atherosclerosis, whereas evidence for those TFA associated with ruminant-derived dairy products and meat remain equivocal. In this study, we investigate the impact of maternal consumption of dietary PHVO (P) and ruminant milk fat (R) on the development of atherosclerosis in their offspring, using the transgenic apoE*3 Leiden mouse. Dams were fed either chow (C) or one of three high-fat diets: a diet reflecting the SFA content of a 'Western' diet (W) or one enriched with either P or R. Diets were fed during either pregnancy alone or pregnancy and lactation. Weaned offspring were then transferred to an atherogenic diet for 12 weeks. Atherosclerosis was assessed as lipid staining in cross-sections of the aorta. There was a significant effect of maternal diet during pregnancy on development of atherosclerosis (P=0·013) in the offspring with those born of mothers fed R or P during pregnancy displaying smaller lesions that those fed C or W. This was not associated with changes in total or lipoprotein cholesterol. Continuing to feed P during lactation increased atherosclerosis compared with that seen in offspring of dams fed P only during pregnancy (P<0·001). No such effect was seen in those from mothers fed R (P=0·596) or W (P=901). We conclude that dietary TFA have differing effects on cardiovascular risk at different stages of the lifecycle.

  4. Both transient and continuous corticosterone excess inhibit atherosclerotic plaque formation in APOE*3-leiden.CETP mice.

    Directory of Open Access Journals (Sweden)

    Hanna E Auvinen

    Full Text Available INTRODUCTION: The role of glucocorticoids in atherosclerosis development is not clearly established. Human studies show a clear association between glucocorticoid excess and cardiovascular disease, whereas most animal models indicate an inhibitory effect of glucocorticoids on atherosclerosis development. These animal models, however, neither reflect long-term glucocorticoid overexposure nor display human-like lipoprotein metabolism. AIM: To investigate the effects of transient and continuous glucocorticoid excess on atherosclerosis development in a mouse model with human-like lipoprotein metabolism upon feeding a Western-type diet. METHODS: Pair-housed female APOE*3-Leiden.CETP (E3L.CETP mice fed a Western-type containing 0.1% cholesterol for 20 weeks were given corticosterone (50 µg/ml for either 5 (transient group or 17 weeks (continuous group, or vehicle (control group in the drinking water. At the end of the study, atherosclerosis severity, lesion area in the aortic root, the number of monocytes adhering to the endothelial wall and macrophage content of the plaque were measured. RESULTS: Corticosterone treatment increased body weight and food intake for the duration of the treatment and increased gonadal and subcutaneous white adipose tissue weight in transient group by +35% and +31%, and in the continuous group by +140% and 110%. Strikingly, both transient and continuous corticosterone treatment decreased total atherosclerotic lesion area by -39% without lowering plasma cholesterol levels. In addition, there was a decrease of -56% in macrophage content of the plaque with continuous corticosterone treatment, and a similar trend was present with the transient treatment. CONCLUSION: Increased corticosterone exposure in mice with human-like lipoprotein metabolism has beneficial, long-lasting effects on atherosclerosis, but negatively affects body fat distribution by promoting fat accumulation in the long-term. This indicates that the increased

  5. Quality of life in elderly Iranian population using leiden-padua questionnaire: A systematic review and meta-analysis

    Directory of Open Access Journals (Sweden)

    Parvin Cheraghi

    2017-01-01

    Full Text Available The world population is getting old rapidly; the aging population is the new phenomenon in Iran too. The aim of this meta-analysis was to estimate the overall and subscales mean score of quality of life (QOL based on the Leiden-Padua (LEIPAD questionnaire among the elderly population of Iran. The major international and national databases including; Medlin, Scopus, Science Direct, SID, MagIran, IranMedex, and Irandoc was searched. All cross-sectional studies, which measured the QOL among the elderly population in Iran using the LEIPAD questionnaire, were included. Furthermore, we used the following key words, “Quality of life,” “aging,” “aged,” “elderly,” and “Iran.” Of 2155 records, four articles reminded for the meta-analysis, which involved 628 participants with a mean age of 71.73 ± 4.28 years. The mean scores of QOL in each scale were as follows: 10.80 (9.30–12.31 for physical health, 13.51 (6.81–20.21 for self-care scale, 8.60 (5.07–12.14 for depression and anxiety, 12.48 (10.39–14.58 for cognitive functioning scale, 2.19 (0.67–3.72 for sexual functioning scale, 10.98 (5.87–16.09 for life satisfaction scale, and 5.90 (3.64–8.16 for social desirability scale. This study revealed that the total QOL for the elderly population is relatively low in Iranian society. It is appeared to provide social support, and upgrade their QOL seems to be essential for the elderly population.

  6. High blood pressure, physical and cognitive function, and risk of stroke in the oldest old: the Leiden 85-plus Study.

    Science.gov (United States)

    Sabayan, Behnam; van Vliet, Peter; de Ruijter, Wouter; Gussekloo, Jacobijn; de Craen, Anton J M; Westendorp, Rudi G J

    2013-01-01

    Epidemiological studies have shown mixed findings on the association between hypertension and stroke in the oldest old. Heterogeneity of the populations under study may underlie variation in outcomes. We examined whether the level of physical and cognitive function moderates the association between blood pressure and stroke. We included 513 subjects aged 85 years old from the population-based Leiden 85-plus Study. Systolic blood pressure, diastolic blood pressure, mean arterial pressure, and pulse pressure were measured at baseline. Activities of daily living and Mini-Mental State Examination were assessed to estimate level of physical and cognitive function, respectively. Five-year risk of stroke was estimated with Cox regression analysis. In the entire cohort, there were no associations between various measures of blood pressure and risk of stroke except for the inverse relation between pulse pressure and stroke risk (hazard ratio [HR], 0.80 [95% confidence interval [CI], 0.66-0.98]). Among subjects with impaired physical functioning, higher systolic blood pressure (HR, 0.74 [95% CI, 0.59-0.92]), mean arterial pressure (HR: 0.68 [95% CI, 0.47-0.97]), and pulse pressure (HR, 0.71 [95% CI, 0.55-0.93]) were associated with reduced risk of stroke. Likewise, among subjects with impaired cognitive functioning, higher systolic blood pressure was associated with reduced risk of stroke (HR, 0.80 [95% CI, 0.65-0.98]). In subjects with unimpaired cognitive functioning, higher diastolic blood pressure (HR: 1.98 [95% CI, 1.21-3.22]) and mean arterial pressure (HR, 1.70 [95% CI, 1.08-2.68]) were associated with higher risk of stroke. Our findings suggest that impaired physical and cognitive function moderates the association between blood pressure and stroke.

  7. Fenofibrate increases very low density lipoprotein triglyceride production despite reducing plasma triglyceride levels in APOE*3-Leiden.CETP mice.

    Science.gov (United States)

    Bijland, Silvia; Pieterman, Elsbet J; Maas, Annemarie C E; van der Hoorn, José W A; van Erk, Marjan J; van Klinken, Jan B; Havekes, Louis M; van Dijk, Ko Willems; Princen, Hans M G; Rensen, Patrick C N

    2010-08-13

    The peroxisome proliferator-activated receptor alpha (PPARalpha) activator fenofibrate efficiently decreases plasma triglycerides (TG), which is generally attributed to enhanced very low density lipoprotein (VLDL)-TG clearance and decreased VLDL-TG production. However, because data on the effect of fenofibrate on VLDL production are controversial, we aimed to investigate in (more) detail the mechanism underlying the TG-lowering effect by studying VLDL-TG production and clearance using APOE*3-Leiden.CETP mice, a unique mouse model for human-like lipoprotein metabolism. Male mice were fed a Western-type diet for 4 weeks, followed by the same diet without or with fenofibrate (30 mg/kg bodyweight/day) for 4 weeks. Fenofibrate strongly lowered plasma cholesterol (-38%) and TG (-60%) caused by reduction of VLDL. Fenofibrate markedly accelerated VLDL-TG clearance, as judged from a reduced plasma half-life of glycerol tri[(3)H]oleate-labeled VLDL-like emulsion particles (-68%). This was associated with an increased post-heparin lipoprotein lipase (LPL) activity (+110%) and an increased uptake of VLDL-derived fatty acids by skeletal muscle, white adipose tissue, and liver. Concomitantly, fenofibrate markedly increased the VLDL-TG production rate (+73%) but not the VLDL-apolipoprotein B (apoB) production rate. Kinetic studies using [(3)H]palmitic acid showed that fenofibrate increased VLDL-TG production by equally increasing incorporation of re-esterified plasma fatty acids and liver TG into VLDL, which was supported by hepatic gene expression profiling data. We conclude that fenofibrate decreases plasma TG by enhancing LPL-mediated VLDL-TG clearance, which results in a compensatory increase in VLDL-TG production by the liver.

  8. Plasminogen activator inhibitor-1 4G/5G polymorphism, factor V Leiden, prothrombin mutations and the risk of VTE recurrence.

    Science.gov (United States)

    Sundquist, Kristina; Wang, Xiao; Svensson, Peter J; Sundquist, Jan; Hedelius, Anna; Larsson Lönn, Sara; Zöller, Bengt; Memon, Ashfaque A

    2015-11-25

    Plasminogen-activator inhibitor (PAI)-1 is an important inhibitor of the plasminogen/plasmin system. PAI-1 levels are influenced by the 4G/5G polymorphism in the PAI-1 promoter. We investigated the relationship between the PAI-1 polymorphism and VTE recurrence, and its possible modification by factor V Leiden (FVL) and prothrombin (PTM) mutations. Patients (n=1,069) from the Malmö Thrombophilia Study were followed from discontinuation of anticoagulant treatment until diagnosis of VTE recurrence or the end of the study (maximum follow-up 9.8 years). One hundred twenty-seven patients (11.9 %) had VTE recurrence. PAI-1 was genotyped by TaqMan PCR. Cox regression analysis adjusted for age, sex and acquired risk factors of VTE showed no evidence of an association between PAI-1 genotype and risk of VTE recurrence in the study population as a whole. However, by including an interaction term in the analysis we showed that FVL but not PTM modified the effect of PAI-1 genotype: patients with the 4G allele plus FVL had a higher risk of VTE recurrence [hazard ratio (HR) =2.3, 95 % confidence interval (CI) =1.5-3.3] compared to patients with the 4G allele but no FVL (reference group) or FVL irrespective of PAI-1 genotype (HR=1.8, 95 % CI=1.3-2.5). Compared to reference group, 5G allele irrespective of FVL was associated with lower risk of VTE recurrence only when compared with 4G allele together with FVL. In conclusion, FVL has a modifying effect on PAI-1 polymorphism in relation to risk of VTE recurrence. The role of PAI-1 polymorphism as a risk factor of recurrent VTE may be FVL dependent.

  9. Report on the International Workshop “The Golden Horde in a Global Perspective: Imperial Strategies”, Leiden University, May 7–8, 2015 »

    Directory of Open Access Journals (Sweden)

    Roman Hautala

    2015-09-01

    Full Text Available This article contains a report on the international conference organized by the University of Leiden in May 2015. According to its organizers, this conference has become the first Western European symposium dedicated to the study of the long history of the Golden Horde. The conference became a fruitful meeting place of the leading Russian and Tatar historians and archaeologists with their Western colleagues from the Netherlands, United Kingdom, United States of America, Hungary, Poland, and Finland. International participants of the Leiden conference discussed the most topical issues of historiography associated with eventful history of the Golden Horde. In particular, the conference participants paid special attention to the study of the administration of the ulus of Jochi that naturally combined governmental models of the Mongol Empire of the Chingizids with local administrative elements. A separate section of the conference was devoted to the analysis of the religious policy of the Golden Horde khans, where special attention was paid to the historiographical debate on the Islamization of the ulus of Jochi. The conference participants also paid considerable attention to the consideration of the material culture of the Golden Horde known both from written sources and, above all, from the materials of archaeological findings. Also, a number of reports of the confe­rence was devoted to foreign and domestic policies of the Golden Horde rulers, at the same time reflecting the central geopolitical role of the ulus of Jochi on the Eurasian continent and the high level of civilization of the Golden Horde. The main achievement of the Leiden conference was a statement on the need of deeper study of the Golden Horde history in order to overcome a number of stereotypes regarding misconceptions of the ulus of Jochi represented as backward and parasitic State formation. The conference participants agreed in general opinion about the necessity of similar

  10. Risk of placenta-mediated pregnancy complications or pregnancy-related VTE in VTE-asymptomatic families of probands with VTE and heterozygosity for factor V Leiden or G20210 prothrombin mutation.

    Science.gov (United States)

    Cordoba, Iris; Pegenaute, Carlota; González-López, Tomás José; Chillon, Carmen; Sarasquete, Maria Eugenia; Martin-Herrero, Francisco; Guerrero, Carmen; Cabrero, Mónica; Garcia Sanchez, Maria Helena; Pabon, Pedro; Lozano, Francisco Santiago; Gonzalez, Marcos; Alberca, Ignacio; González-Porras, José Ramón

    2012-09-01

    Few studies have evaluated the risk of pregnancy-related adverse events in asymptomatic relatives of probands for VTE and factor V Leiden or the G20210A variant. The antepartum management of this population ranges from antepartum anticoagulation therapy to clinical surveillance. To evaluate the risk of placenta-mediated pregnancy complications and pregnancy-related VTE in VTE-asymptomatic families of probands with VTE and who are heterozygous carriers of either factor V Leiden or PT-G20210A mutation. One hundred and fifty-eight relatives, who had 415 pregnancies, were retrospectively evaluated. Odds ratios and 95% confidence intervals were calculated to compare pregnancy outcomes between women with and without thrombophilia. In the factor V Leiden group, 22 placenta-mediated pregnancy events of 152 pregnancies (14.4%) were reported, compared with 25 adverse events of 172 pregnancies in the G20210A prothrombin group (14.5%) and 13 adverse events of 91 pregnancies in the non-carrier group (14.2%). Carriers of factor V Leiden or G20210A prothrombin were not associated with a higher risk of pregnancy-adverse outcomes compared with non-carriers: OR 1.02 (95% CI, 0.40-2.25) and 1.25 (95% CI, 0.48-3.24), respectively. Four episodes of pregnancy-associated VTE of 415 pregnancies (0.96%) were recorded. Two episodes of VTE in the G20210A group, one in the factor V Leiden group, and one episode in the non-carrier group were noted. In VTE-asymptomatic relatives of probands with VTE, the presence of factor V Leiden or the G20210A prothrombin mutation in heterozygosis should not lead to a decision to instigate antepartum prophylaxis. © 2012 John Wiley & Sons A/S.

  11. Lipidomics reveals multiple pathway effects of a multi-components preparation on lipid biochemistry in ApoE*3Leiden.CETP mice.

    Directory of Open Access Journals (Sweden)

    Heng Wei

    Full Text Available BACKGROUND: Causes and consequences of the complex changes in lipids occurring in the metabolic syndrome are only partly understood. Several interconnected processes are deteriorating, which implies that multi-target approaches might be more successful than strategies based on a limited number of surrogate markers. Preparations from Chinese Medicine (CM systems have been handed down with documented clinical features similar as metabolic syndrome, which might help developing new intervention for metabolic syndrome. The progress in systems biology and specific animal models created possibilities to assess the effects of such preparations. Here we report the plasma and liver lipidomics results of the intervention effects of a preparation SUB885C in apolipoprotein E3 Leiden cholesteryl ester transfer protein (ApoE*3Leiden.CETP mice. SUB885C was developed according to the principles of CM for treatment of metabolic syndrome. The cannabinoid receptor type 1 blocker rimonabant was included as a general control for the evaluation of weight and metabolic responses. METHODOLOGY/PRINCIPAL FINDINGS: ApoE*3Leiden.CETP mice with mild hypercholesterolemia were divided into SUB885C-, rimonabant- and non-treated control groups. SUB885C caused no weight loss, but significantly reduced plasma cholesterol (-49%, p<0.001, CETP levels (-31%, p<0.001, CETP activity (-74%, p<0.001 and increased HDL-C (39%, p<0.05. It influenced lipidomics classes of cholesterol esters and triglycerides the most. Rimonabant induced a weight loss (-9%, p<0.05, but only a moderate improvement of lipid profiles. In vitro, SUB885C extract caused adipolysis stimulation and adipogenesis inhibition in 3T3-L1 cells. CONCLUSIONS: SUB885C, a multi-components preparation, is able to produce anti-atherogenic changes in lipids of the ApoE*3Leiden.CETP mice, which are comparable to those obtained with compounds belonging to known drugs (e.g. rimonabant, atorvastatin, niacin. This study successfully

  12. Antes da Queda do Muro, além dele e sem ele: A estética emergente em Die neuen Leiden...

    OpenAIRE

    2010-01-01

    This article is an analysis of the novel Die neuen Leiden des jungen W. (1973) in the light of its intertextuality with Goethe’s, Defoe’s and Salinger’s texts. It has been suggested that Plenzdorf's novel creates a stylistic and ideological world, which is instigating for the literary paradigms of the Socialist Realism of the 70s in East Germany. Twenty years after the Fall of the Berlin Wall, this created world in the novel seems to be up-to-date or even paradigmatic for the current repre...

  13. Low prevalence of Factor V Leiden and the prothrombin G20210A mutation in a healthy population from the central-south region of Chile Baixa prevalência do Fator V Leiden e da mutação da protrombina G20210A em uma população sã da região centro-sul do Chile

    Directory of Open Access Journals (Sweden)

    Iván Palomo

    2009-01-01

    Full Text Available Thrombosis is a result of the interaction between predisposing genetic polymorphisms and acquired risk factors. The two prothrombotic polymorphisms which are most frequently associated with thrombosis are factor V (FV Leiden and the prothrombin (PT G20210A mutation. The objective of this work was to investigate the prevalence of both factors in the central-south region of Chile. Determination of the frequency was carried out by means of a genetic analysis of 1200 healthy, non-native individuals. The mutation of FV Leiden was found in 1.25% of the population and the PT G20210A mutation in 1.33%. None of the individuals were homozygosis or had both polymorphisms. The prevalences of FV Leiden and the PT G20210A mutation are less common in the healthy population.A trombose pode ser o resultado da interação de polimorfismo genético e fatores de riscos adquiridos. Os polimosfirmos protrombóticos mais frequentes são fator V (FV Leiden e a mutação da protrombina (PT G20210A. O objetivo deste trabalho foi investigar a prevalência de ambos os polimorfismos na região centro-sul do Chile. Foram realizadas análises genéticas (PCR RFLP de 1.200 pessoas saudáveis, não nativas da região. Foram encontrados 1,25% de mutação do Fator V Leiden e 1,33% da mutação da protrombina G20210A. Não foi detectada homozigose em ambos os polimorfismos. A prevalência de FV Leiden e da mutação G20210A é baixa na população estudada.

  14. Anécdotas castellanas en escritura hebraica. Apuntes paremiológicos conservados en las anotaciones hebreas de Alfonso de Zamora (Ms. Leiden Or. 645

    Directory of Open Access Journals (Sweden)

    Alonso Fontela, Carlos

    2011-12-01

    Full Text Available Problems of translation and interpretation are addressed in this presentation of Alfonso de Zamora’s Hebrew and Hebrew “Aljamiado” Spanish notes (ca. 1530 from Leiden University Library Ms. Or. 645 (ol. Warner 65 containing anecdotes, sayings and short tales. Additional notes dealing with a manuscript copy of the Targum to the Prophets comissioned by the University of Salamanca are also discussed.

    Este artículo estudia algunas notas manuscritas de Alfonso de Zamora en hebreo y en aljamía hebraico-castellana datadas hacia 1530, y procedentes del ms. Or. 645 (ol. Warner 65 de la Biblioteca de la Universidad de Leiden. Dichas notas contienen anécdotas, refranes y «cuentecillos», tales como las anécdotas «de fray Palomo», «de un portugués», y «de lo que dijo el gallego». Otras anotaciones de Zamora tratan de un trabajo de copia del Targum a los Profetas por encargo de la Universidad de Salamanca.

  15. A genetic study of Factor V Leiden (G1691A) mutation in young ischemic strokes with large vessel disease in a South Indian population.

    Science.gov (United States)

    Anadure, Ravi; Christopher, Rita; Nagaraja, Dindagur; Narayanan, Coimbatore

    2017-10-01

    Factor V Leiden (FVL) has been, by far, the most investigated gene mutation, with 26 studies to date, on its role in arterial strokes. Overall, a meta-analysis of all these studies taken together showed that carriers of the Factor V Leiden allele were 1.33times more likely to develop arterial strokes when compared to controls. We subjected a highly select subset of young strokes, with large vessel infarcts, to genetic analysis for FVL mutation and compared them with matched healthy controls to look for a statistically significant association. In this prospective study, 6/120 cases (5%) and 2/120 controls (1.6%) were positive for heterozygous FVL (G1691A) mutation. The higher prevalence of FVL mutation in cases (5%) compared to controls (1.6%) did not show statistical significance with a Pearson's Chi square P value of 0.15. The Odds Ratio (OR) for risk of large vessel disease in FVL positive cases was 3.10 (95% CI of 0.61-15.7). FVL mutation (G1691A) in young Indian subjects with ischemic strokes does not seem to be significantly associated with large vessel disease. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Prevalence of Factor V Leiden-G1691A and MTHFR-C677T Thrombosis Gene Modifier in Iron Deficiency Anemia: A Pathophysiological Effect in Indian Isolates.

    Science.gov (United States)

    Pandey, S K; Pandey, S; Mishra, R M; Indurkar, M

    2017-03-01

    Normal iron levels are required to prevent thrombocytosis by inhibiting thrombopoiesis. Thrombocytosis is usually associated with a mild iron deficiency and is the result of a lack of inhibition of thrombopoiesis. Study participants were 430 iron deficiency anemia (IDA) patients. Ten (10) mL of venous blood were collected for the subjects. Ferritin analysis was done by ELISA method while Hemogram analysis was done by auto-analyzer. Factor V Leiden, PRTG20210A, and MTHFR C677T genotype analysis was performed by PCR-RFLP method. Among the patients, 9 were heterozygous (G>A) and 2 were homozygous (A>A) carrier of FV Leiden; while 20 were heterozygous (C>T) and 3 were homozygous (T>T) for MTHFR polymorphism. None of the patient was identified with PT mutation. Patients with thrombosis gene marker had lower hemoglobin, mean corpuscular volume, mean corpuscular haemoglobin levels, and mean corpuscular hemoglobin concentration than patients without thrombosis gene marker. Serum ferritin was elevated in subject with the absence of thrombosis gene markers. Our data suggest a high impact of inherited hypercoagulability risk factors in the pathogenesis of IDA and its complications.

  17. Teaching Engineering Ethics to PhD Students: A Berkeley-Delft Initiative : Commentary on "Ethics Across the Curriculum: Prospects for Broader (and Deeper) Teaching and Learning in Research and Engineering Ethics".

    Science.gov (United States)

    Taebi, Behnam; Kastenberg, William E

    2016-07-13

    A joint effort by the University of California at Berkeley and Delft University of Technology to develop a graduate engineering ethics course for PhD students encountered two types of challenges: academic and institutional. Academically, long-term collaborative research efforts between engineering and philosophy faculty members might be needed before successful engineering ethics courses can be initiated; the teaching of ethics to engineering graduate students and collaborative research need to go hand-in-hand. Institutionally, both bottom-up approaches at the level of the faculty and as a joint research and teaching effort, and top-down approaches that include recognition by a University's administration and the top level of education management, are needed for successful and sustainable efforts to teach engineering ethics.

  18. The Frequency of Factor V Leiden, Prothrombin G20210A and Methylenetetrahydrofolate Reductase C677T Mutations in Migraine Patients

    Directory of Open Access Journals (Sweden)

    Ruhsen Öcal

    2010-12-01

    Full Text Available OBJECTIVE: Migraine is an independent risk factor for ischemic stroke, but its pathophysiology is still unclear. Genetic factors that predispose patients to thrombosis have been studied in patients with migraine to highlight the pathogenesis, but the results remain controversial. In this study, the frequencies of factor V Leiden (FVL, prothrombin (Pt G20210A and methylenetetrahydrofolate reductase (MTHFR C677T mutations were investigated. METHODS: One hundred and sixty patients aged of 15 to 55 years with no history of systemic disease and who had been diagnosed as migraine according to the International Headache Society (IHS diagnostic criteria at Baskent University Hospital Neurology Outpatient Clinics were investigated for FVL, Pt G20210A and MTHFR C677T mutations from their genomic DNA, and the results were compared with those of healthy controls. RESULTS: One hundred and fifty five (96.9% of 160 migraine patients were homozygote normal, 5 (3.1% were heterozygote and none of them were homozygote mutant for FVL. The control group had 9.8% heterozygote individuals but the difference between the percentages was not statistically significant (p> 0.05. There were no homozygote mutant individuals in the Turkish population study in normal subjects like our study. Thirty nine (24.4% of 160 migraine patients were heterozygote and 8 (5% were homozygote mutant for MTHFR C677T. The control group had 37 (34.9% heterozygote and 6 (5.6% homozygote mutant individuals. The difference between the percentages was not statistically significant (p= 0.15. Three (1.9% of 160 migraine patients were heterozygote and 5 (2.9% of the control group were heterozygote mutant for Pt G20210A mutation. The control group had 37 (34.9% heterozygote and 6 (5.6% homozygote mutant individuals. The difference between the percentages was not statistically significant (p= 0.420. CONCLUSION: Our study indicates that FVL, Pt G20210A and MTHFR C677T gene mutations, which are considered

  19. Effect of macrophage-derived mouse ApoE, human ApoE3-Leiden, and human ApoE2 (Arg158→Cys) on cholesterol levels and atherosclerosis in ApoE- deficient mice

    NARCIS (Netherlands)

    Eck, M. van; Herijgers, N.; Dijk, K.W. van; Havekes, L.M.; Hofker, M.H.; Groot, P.H.E.; Berkel, T.J.C. van

    2000-01-01

    The effect of monocyte/macrophage-derived wild-type mouse apolipoprotein E (apoE), human apoE3-Leiden, and human apoE2 on serum cholesterol levels and the development of atherosclerosis in apoE-deficient (apoe-/-) mice was investigated by using bone marrow transplantation (BMT). At 4 weeks after

  20. No interaction between factor V Leiden and hyperhomocysteinemia or MTHFR 677TT genotype in venous thrombosis. Results of a meta-analysis of published studies and a large case-only study.

    NARCIS (Netherlands)

    Keijzer, M.B.; Borm, G.F.; Blom, H.J.; Bos, G.M.; Rosendaal, F.R.; Heijer, M. den

    2007-01-01

    Homocysteine may have a thrombogenic effect through inhibition of inactivation of factor Va by activated protein C. Because factor V Leiden also leads to resistance of factor V to activated protein C, it would be possible that both factors show interaction for the risk of venous thrombosis. This has

  1. Co-segregation of thrombophilic disorders in factor V Leiden carriers; the contributions of factor VIII, factor XI, thrombin activatable fibrinolysis inhibitor and lipoprotein(a) to the absolute risk of venous thromboembolism

    NARCIS (Netherlands)

    Libourel, EJ; Bank, [No Value; Meinardi, [No Value; Balje-Volkers, CP; Hamulyak, K; Middeldorp, S; Koopman, MMW; van Pampus, ECM; Prins, MH; Buller, HR; van der Meer, J

    2002-01-01

    Background and Objectives. The clinical expression of factor V Leiden varies widely within and between families and only a minority of carriers will ever develop venous thromboembolism. Co-segregation of thrombophilic disorders is a possible explanation, Our aim was to assess the contributions of

  2. Different risk of deep vein thrombosis and pulmonary embolism in carriers with factor V Leiden compared with non-carriers, but not in other thrombophilic defects. Results from a large retrospective family cohort study

    NARCIS (Netherlands)

    Makelburg, Anja B. U.; Veeger, Nic J. G. M.; Middeldorp, Saskia; Hamulyak, Karly; Prins, Martin H.; Buller, Harry R.; Lijfering, Willem M.

    2010-01-01

    The term factor V Leiden (FVL) paradox is used to describe the different risk of deep vein thrombosis and pulmonary embolism that has been found in carriers of FVL. In a thrombophilic family-cohort, we estimated differences in absolute risks of deep vein thrombosis and pulmonary embolism for various

  3. Factor V Leiden mutation, prothrombin gene mutation, and deficiencies in coagulation inhibitors associated with Budd-Chiari syndrome and portal vein thrombosis: results of a case-control study

    NARCIS (Netherlands)

    H.L.A. Janssen (Harry); J.P. Vandenbroucke; F.R. Rosendaal (Frits); B. van Hoek (Bart); J.R. Meinardi; F.P. Vleggaar (Frank); S.H. van Uum; E.B. Haagsma (Els); F.J.M. van der Meer; J. van Hattum (Jan); R.A. Chamuleau; R.P.R. Adang (Rob)

    2000-01-01

    textabstractIn a collaborative multicenter case-control study, we investigated the effect of factor V Leiden mutation, prothrombin gene mutation, and inherited deficiencies of protein C, protein S, and antithrombin on the risk of Budd-Chiari syndrome (BCS) and portal vein thrombosi

  4. Factor V Leiden

    Science.gov (United States)

    ... increase your chance of developing abnormal blood clots (thrombophilia), usually in your veins. Most people with factor ... sharp tools. References Bauer KA. Management of inherited thrombophilia. http://www.uptodate.com/home. Accessed June 6, ...

  5. The Prevalence of Factor V Leiden (G1691A) and Methylenetetrahydrofolate Reductase C677T Mutations in Sickle Cell Disease in Western India.

    Science.gov (United States)

    Kangne, Harshada K; Jijina, Farah F; Italia, Yazdi M; Jain, Dipti L; Nadkarni, Anita H; Ghosh, Kanjaksha K; Colah, Roshan B

    2015-03-01

    The prevalence of the Factor V Leiden (FVL; G1691A) mutation and the methylenetetrahydrofolate reductase (MTHFR; C677T) mutation was determined in 180 patients with sickle cell (SS) disease (126 sickle homozygous and 54 sickle β-thalassaemia--age 1-47 years) and in 130 healthy controls. The FVL mutation in the heterozygous state was present in only 3 patients with SS disease and was absent in the controls. Genotyping of MTHFR 677C > T revealed increased frequency of the C allele than the T allele in patients as well as in controls. This suggests that these genetic markers may not be major risk factors for a hypercoagulable state in Indian patients with SS disease. © The Author(s) 2013.

  6. The relationship of the factor V Leiden mutation or the deletion-deletion polymorphism of the angiotensin converting enzyme to postoperative thromboembolic events following total joint arthroplasty

    Directory of Open Access Journals (Sweden)

    Fang Carrie

    2001-04-01

    Full Text Available Abstract Background Although all patients undergoing total joint arthroplasty are subjected to similar risk factors that predispose to thromboembolism, only a subset of patients develop this complication. The objective of this study was to determine whether a specific genetic profile is associated with a higher risk of developing a postoperative thromboembolic complication. Specifically, we examined if the Factor V Leiden (FVL mutation or the deletion polymorphism of the angiotensin-converting enzyme (ACE gene increased a patient's risk for postoperative thromboembolic events. The FVL mutation has been associated with an increased risk of idiopathic thromboembolism and the deletion polymorphism of the ACE gene has been associated with increased vascular tone, attenuated fibrinolysis and increased platelet aggregation. Methods The presence of these genetic profiles was determined for 38 patients who had a postoperative symptomatic pulmonary embolus or proximal deep venous thrombosis and 241 control patients without thrombosis using molecular biological techniques. Results The Factor V Leiden mutation was present in none of the 38 experimental patients and in 3% or 8 of the 241 controls (p = 0.26. Similarly there was no difference detected in the distribution of polymorphisms for the ACE gene with the deletion-deletion genotype present in 36% or 13 of the 38 experimental patients and in 31% or 74 of the 241 controls (p = 0.32. Conclusions Our results suggest that neither of these potentially hypercoaguable states are associated with an increased risk of symptomatic thromboembolic events following total hip or knee arthroplasty in patients receiving pharmacological thromboprophylaxis.

  7. Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India.

    Science.gov (United States)

    Nishank, Sudhansu Sekhar; Singh, Mendi Prema Shyam Sunder; Yadav, Rajiv

    2013-11-01

    It is known that patients with sickle cell disease (SCD) present activation of the blood coagulation and fibrinolytic systems, especially during vaso-occlusive crises and also during the steady state of the disease. We determined whether the presence of the factor prothrombin gene G20210A variant, factor V gene G1691A mutation (factor V Leiden), and methylenetetrahydrofolate reductase (MTHFR) C677T polymorphisms may be risk factors for vascular complications in individuals with SCD. The study involved 150 patients with sickle cell anemia and 150 healthy controls of Central India. Genotyping of three thrombophilic mutations was carried out by PCR-RFLP methods using MnlI, Hind III, and Hinf I, respectively, for factor V Leiden, prothrombin, and MTHFR mutations. Patients with SCD had significantly higher prevalence of mutant variants of MTHFR gene (28.0% heterozygotes and 14.6% homozygotes) and FVL gene (14.6% heterozygotes) as compared to normal/control individuals, but complete absence of mutant variants of prothrombin gene. The patients with SCD having mutant variants of MTHFR and FVL genes showed higher incidence of pain in chest, abdomen, and bone joints along with early age of onset of clinical manifestations as well as frequent dependence on blood transfusion than those patients with SCD having wild variants of these thrombotic genes. As compared to control subjects, SCD individuals having mutant variants of FVL and MTHFR genes had significant association with higher levels of prothrombin fragment (F1+2), D-dimer, thrombin-antithrombin (TAT), and lower level of protein C. MTHFR C677T and FVL G1691A polymorphisms may be risk factors for increased vascular complications in patient with SCD. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  8. Cerebral infarction and femoral venous thrombosis detected in a patient with diabetic ketoacidosis and heterozygous factor V Leiden G1691A and PAI-1 4G/5G mutations.

    Science.gov (United States)

    Yaroglu Kazanci, Selcen; Yesilbas, Osman; Ersoy, Melike; Kihtir, Hasan Serdar; Yildirim, Hamdi Murat; Sevketoglu, Esra

    2015-09-01

    Cerebral infarction is one of the serious neurological complications of diabetic ketoacidosis (DKA). Especially in patients who are genetically prone to thrombosis, cerebral infarction may develop due to inflammation, dehydration, and hyperviscocity secondary to DKA. A 6-year-old child with DKA is diagnosed with cerebral infarction after respiratory insufficiency, convulsion, and altered level of consciousness. Femoral and external iliac venous thrombosis also developed in a few hours after central femoral catheter had been inserted. Heterozygous type of factor V Leiden and PAI-14G/5G mutation were detected. In patients with DKA, cerebral infarction may be suspected other than cerebral edema when altered level of consciousness, convulsion, and respiratory insufficiency develop and once cerebral infarction occurs the patients should also be evaluated for factor V Leiden and PAI-14G/5G mutation analysis in addition to the other prothrombotic risk factors.

  9. Pulmonary Embolism in a Sarcoidosis Patient Double Heterozygous for Methylenetetrahydrofolate Reductase Gene Polymorphisms and Factor V Leiden and Homozygous for the D-Allele of Angiotensin Converting Enzyme Gene

    Directory of Open Access Journals (Sweden)

    Nadim El-Majzoub

    2015-01-01

    Full Text Available Sarcoidosis is a multisystem granulomatous disease of unknown etiology and pathogenesis. It presents in patients younger than 40 years of age. The lungs are the most commonly affected organ. Till the present day, there is no single specific test that will accurately diagnose sarcoidosis; as a result, the diagnosis of sarcoidosis relies on a combination of clinical, radiologic, and histologic findings. Patients with sarcoidosis have been found to have an increased risk of pulmonary embolism compared to the normal population. MTHFR and factor V Leiden mutations have been reported to increase the risk of thrombosis in patients. We hereby present a case of a middle aged man with sarcoidosis who developed a right main pulmonary embolism and was found to be double heterozygous for methylenetetrahydrofolate reductase gene polymorphisms and factor V Leiden and homozygous for the D-allele of the angiotensin converting enzyme gene.

  10. Evaluation of maternal serum folate, vitamin B12, and homocysteine levels andfactor V Leiden, factor II g.20210G>A, and MTHFR variations in prenatallydiagnosed neural tube defects.

    Science.gov (United States)

    Aydin, Hatip; Arisoy, Resul; Karaman, Ali; Erdoğdu, Emre; Çetinkaya, Arda; B Geçkinli, Bilge; Şimşek, Hasan; Demirci, Oya

    2016-02-17

    Neural tube defects (NTDs) are common congenital malformations that develop as a result of interactions between several genes and environmental factors. Many factors have been investigated in order to understand the etiology of NTDs, and many studies have identified folate intake as a common contributing factor. The exact etiology of the disease is still unknown. In this study, we compared serum folate, vitamin B12, and homocysteine levels, along with common thrombophilia-related genetic variations, including factor V Leiden, factor II g.20210G>A, MTHFR c.677C>T, and MTHFR c.1298A>C, in 35 pregnant women with fetal NTDs and 38 pregnant women with healthy fetuses. A significant difference in serum vitamin B12 level and factor V Leiden frequency was detected between the two groups. On the other hand, serum folate, homocysteine levels, and factor II g.20210G>A, MTHFR c.677C>T, and MTHFR c.1298A>C were not significantly different in the NTD group compared to the controls. These results indicate that vitamin B12 supplementation along with folate may help in lowering NTD frequency. In addition, this is the first study that provides evidence for a possible relationship between increased NTD risk and factor V Leiden.

  11. Leiden reconstructed human epidermal model as a tool for the evaluation of the skin corrosion and irritation potential according to the ECVAM guidelines.

    Science.gov (United States)

    El Ghalbzouri, A; Siamari, R; Willemze, R; Ponec, M

    2008-08-01

    In the ECVAM validation studies two common skin protocols have been developed, the skin corrosion and skin irritation protocol. Both protocols include next to general and functional conditions that the skin model must meet, also the correct prediction of the activity of certain reference chemicals. For the skin corrosion protocol, the OECD TG 431 defined 12 reference chemicals that should be correctly predicted by the epidermal skin model. For skin irritation 20 test substances should meet the defined criteria. In this study we aimed to subject our Leiden human epidermal (LHE) model to both common protocols according to the ECVAM guidelines. The LHE model generated in this study has been fully characterized and shows very high similarities with the native skin. After minor technical changes in both protocols, corrosion classifications were obtained in concordance with those reported for the validated human skin models EpiSkin and EpiDerm. The results obtained with the common skin irritation protocol were very similar to that of earlier studies with the SkinEthic, EpiSkin and EpiDerm models. This means that the protocols and prediction models developed during the validation studies with a specific skin model can be used with other similar skin models. This study demonstrates that reconstructed human skin equivalents have been proven to be efficient and reliable alternatives to animal testing.

  12. Deep vein thrombosis, ecythyma gangrenosum and heparin-induced thrombocytopenia occurring in a man with a heterozygous Factor V Leiden mutation

    Directory of Open Access Journals (Sweden)

    Mariya Apostolova

    2012-11-01

    Full Text Available Skin necrosis and limb gangrene are occasional thrombotic manifestations of anticoagulation therapy. We report a man heterozygous for the Factor V Leiden (FVL mutation, and with a history of recurrent deep venous thrombosis, who initially presented with a necrotic skin lesion of the right flank while on warfarin therapy with a therapeutic international normalized ratio. Warfarin was discontinued and he received intravenous heparin. Thereafter he developed thrombocytopenia and pedal erythema and was diagnosed with heparin-induced thrombocytopenia (HIT. Heparin was replaced with argatroban. He ultimately underwent bilateral below-knee amputations for the thrombotic complications of the HIT. The initial necrotic lesion healed with antibiotics and wound care. Pathologic examination of multiple biopsy specimens revealed two separate lesions. One was necrotic tissue infiltrated with methicillin resistant Staphylococcus aureus having features of ecthyma gangrenosum. The second showed thrombotic changes consistent with HIT. The case illustrates the differential diagnosis of skin necrosis and limb gangrene in patients on warfarin and heparin, and also the clinical complexities that can occur in a FVL heterozygote.

  13. GLP-1 receptor activation inhibits VLDL production and reverses hepatic steatosis by decreasing hepatic lipogenesis in high-fat-fed APOE*3-Leiden mice.

    Directory of Open Access Journals (Sweden)

    Edwin T Parlevliet

    Full Text Available OBJECTIVE: In addition to improve glucose intolerance, recent studies suggest that glucagon-like peptide-1 (GLP-1 receptor agonism also decreases triglyceride (TG levels. The aim of this study was to evaluate the effect of GLP-1 receptor agonism on very-low-density lipoprotein (VLDL-TG production and liver TG metabolism. EXPERIMENTAL APPROACH: The GLP-1 peptide analogues CNTO3649 and exendin-4 were continuously administered subcutaneously to high fat diet-fed APOE*3-Leiden transgenic mice. After 4 weeks, hepatic VLDL production, lipid content, and expression profiles of selected genes involved in lipid metabolism were determined. RESULTS: CNTO3649 and exendin-4 reduced fasting plasma glucose (up to -30% and -28% respectively and insulin (-43% and -65% respectively. In addition, these agents reduced VLDL-TG production (-36% and -54% respectively and VLDL-apoB production (-36% and -43% respectively, indicating reduced production of VLDL particles rather than reduced lipidation of apoB. Moreover, they markedly decreased hepatic content of TG (-39% and -55% respectively, cholesterol (-30% and -55% respectively, and phospholipids (-23% and -36% respectively, accompanied by down-regulation of expression of genes involved in hepatic lipogenesis (Srebp-1c, Fasn, Dgat1 and apoB synthesis (Apob. CONCLUSION: GLP-1 receptor agonism reduces VLDL production and hepatic steatosis in addition to an improvement of glycemic control. These data suggest that GLP-receptor agonists could reduce hepatic steatosis and ameliorate dyslipidemia in patients with type 2 diabetes mellitus.

  14. Maternal factor V Leiden and prothrombin mutations do not seem to contribute to the occurrence of two or more than two consecutive miscarriages in Caucasian patients.

    Science.gov (United States)

    Baumann, Kristin; Beuter-Winkler, Petra; Hackethal, Andreas; Strowitzki, Thomas; Toth, Bettina; Bohlmann, Michael K

    2013-12-01

    We analysed the prevalence of the most common hereditary thrombophilia (hTP) - factor V Leiden (FVL) mutation, prothrombin 20210 G>A substitution (PT) - and the 677 C>T replacement in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene in Caucasian patients with a history of two and more consecutive recurrent miscarriages (RMs) as compared to healthy controls with an identical ethnic background and at least one live birth. A multicenter analysis of three hTP was performed in 641 RM patients identically screened at specialized university centres. The study groups consisted of 240 patients with 2 (1) and 401 patients with >2 miscarriages (2) and were compared with 157 controls. There was no significant difference in the prevalence of the hTP between RM patients and controls nor within the two study groups. Subgroup analysis showed that the homozygous MTHFR polymorphism was significantly more prevalent in the study group 2 as compared to study group 1 (13.9 versus 7.9%, P = 0.02). In Caucasians, maternal FVL or PT mutations do not seem to contribute to the pathophysiology of RM, irrespective of the number of miscarriages. However, the role of the homozygous MTHFR polymorphism merits further investigation. © 2013 John Wiley & Sons Ltd.

  15. Factor V G1691A (Leiden and prothrombin G20210A gene mutation status, and thrombosis in patients with chronic myeloproliferative disorders

    Directory of Open Access Journals (Sweden)

    Nur Soyer

    2011-12-01

    Full Text Available Objective: The aim of this study was to examine Factor V G1691A (Leiden (FVL and prothrombin G20210A (PT gene mutation status, and their relationship with thrombosis in patients with chronic myeloproliferative disorders (CMPDs.Materials and Methods: The study included 160 patients with a CMPD that were regularly followed-up between 1993 and 2009. FVL and PT mutation status was established based on blood samples analyzed via PCR using specific primers.Results: The frequency of FVL and PT mutation was 12.5% and 4.4%, respectively. In total, 27 episodes of thrombosis occurred in 24 (15% of the patients, and there wasn’t an association between the observed thrombotic events, and FVL or PT mutations. Hepatic vein thrombosis was noted in 3 patients that had FVL mutation, of which 1 also had PT mutation.Conclusion: We did not observe a relationship between thrombosis, and FVL or PT mutations in CMPD patients; however, 3 of the patients that had hepatic vein thrombosis also had FVL mutation. Larger studies are needed to more clearly determine if all CMPD patients with hepatic vein thrombosis need be investigated for FVL and PT mutation.

  16. Risk of Budd-Chiari syndrome associated with factor V Leiden and G20210A prothrombin mutation: a meta-analysis.

    Directory of Open Access Journals (Sweden)

    Peijin Zhang

    Full Text Available BACKGROUND: Various studies have demonstrated that factor V Leiden (FVL and G20210A prothrombin mutation contribute to the risk of Budd-Chiari syndrome (BCS, while other studies provided conflicting findings. In order to derive more precise estimations of the relationships, a meta-analysis was performed. METHODS: Eligible articles were identified through search of databases including Pubmed, Chinese Biomedical Database (CBM, Chinese, and Chinese National Knowledge Infrastructure (CNKI, Chinese. Odd ratios (ORs with 95% confidence intervals (CIs were calculated using random- or fixed- model. RESULTS: Finally, twelve studies were included for FVL and nine studies were included for G20210A prothrombin mutation. With respect to FVL, significantly increased BCS risk was found in the overall population (OR = 6.29, 95%CI = 4.23-9.36. Subgroup analyses suggested that FVL was associated with an increased risk of BCS in the population with high background mutation prevalence (>1% in the normal population. No significant association was found between BCS and G20210A prothrombin mutation (OR = 1.78, 95%CI = 0.77-4.11. CONCLUSION: The presence of FVL should be evaluated in patients with BCS. Conversely, G20210A prothrombin mutation is not significantly associated with risk of BCS. Large-scale well designed studies are necessary to be conducted to further confirm or refute the observed association.

  17. Association of factor V Leiden, Janus kinase 2, prothrombin, and MTHFR mutations with primary Budd-Chiari syndrome in Egyptian patients.

    Science.gov (United States)

    El Sebay, Hatem M; Safan, Manal A; Daoud, Ashraf A; Tayel, Safaa I; Nouh, Mohamed A; El Shafie, Shymaa

    2016-01-01

    Budd-Chiari syndrome (BCS) is defined as obstruction of hepatic venous outflow anywhere from the small hepatic veins to the suprahepatic inferior vena cava. The pathogenesis of BCS is still not fully understood. This study aimed to evaluate the association of factor V Leiden (FVL), Janus kinase 2 (JAK2), prothrombin, and methylene tetrahydrofolate reductase (MTHFR) mutations with primary BCS. The study was carried out on 35 patients with primary BCS and 15 age and gender matched healthy individuals as a control group. Genotyping of FVL, prothrombin, and MTHFR mutations was determined by GENEQUALITY AB-THROMBO TYPE kit based on the reverse hybridization principle. JAK2 mutation was determined by polymerase chain reaction-restriction fragment length polymorphism. There was a statistically significant difference between patients and controls regarding FVL, MTHFR C677T, and MTHFR A1298C mutations with odds ratio of 1.83, 2.0, and 1.79, respectively. Hetero MTHFR C677T, hetero FVL, and hetero MTHFR A1298C were the most common etiological factors being responsible for 57.1, 42.9, and 42.9% of primary BCS cases, respectively. It could be concluded that BCS is a multifactorial disease; in the current study, MTHFR C677T mutation was the most common cause of disease. Identification of one cause of BCS should not eliminate investigations for detection of other etiological factors. © 2015 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd.

  18. Thrombophilic risk of individuals with rare compound factor V Leiden and prothrombin G20210A polymorphisms: an international case series of 100 individuals.

    Science.gov (United States)

    Lim, Ming Y; Deal, Allison M; Kim, Steven; Musty, Michael D; Conard, Jacqueline; Simioni, Paolo; Dutrillaux, Fabienne; Eid, Suhair S; Middeldorp, Saskia; Halbmayer, Walter M; Boneu, Bernard; Moia, Marco; Moll, Stephan

    2016-10-01

    The risk of thrombosis in individuals with rare compound thrombophilias, homozygous factor V Leiden (FVL) plus heterozygous prothrombin G20210A (PTM), homozygous PTM plus heterozygous FVL, and homozygous FVL plus homozygous PTM, is unknown. We identified, worldwide, individuals with these compound thrombophilias, predominantly through mailing members of the International Society on Thrombosis and Haemostasis. Physicians were sent a clinical questionnaire. Confirmatory copies of the genetic results were obtained. One hundred individuals were enrolled; 58% were female. Seventy-one individuals had a venous thrombosis (includes superficial and deep vein thrombosis, and pulmonary embolism), 4 had an arterial thrombosis and 6 had both. Nineteen individuals had never had a thrombotic event. Thrombosis-free survival curves demonstrated that 50% of individuals had experienced a thrombotic event by 35 yrs of age, while 50% had a first venous thromboembolic event (VTE; includes all venous thrombosis except superficial thrombosis) by 41 yrs of age; 38.2% of first VTEs were unprovoked. 37% of patients had at least one VTE recurrence. Seventy percent of first pregnancies carried to term and not treated with anticoagulation were thrombosis-free. In conclusion, patients with these rare compound thrombophilias are not exceedingly thrombogenic, even though they have a substantial risk for VTE. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  19. A prepared speech in front of a pre-recorded audience: subjective, physiological, and neuroendocrine responses to the Leiden Public Speaking Task.

    Science.gov (United States)

    Westenberg, P Michiel; Bokhorst, Caroline L; Miers, Anne C; Sumter, Sindy R; Kallen, Victor L; van Pelt, Johannes; Blöte, Anke W

    2009-10-01

    This study describes a new public speaking protocol for youth. The main question asked whether a speech prepared at home and given in front of a pre-recorded audience creates a condition of social-evaluative threat. Findings showed that, on average, this task elicits a moderate stress response in a community sample of 83 12- to 15-year-old adolescents. During the speech, participants reported feeling more nervous and having higher heart rate and sweatiness of the hands than at baseline or recovery. Likewise, physiological (heart rate and skin conductance) and neuroendocrine (cortisol) activity were higher during the speech than at baseline or recovery. Additionally, an anticipation effect was observed: baseline levels were higher than recovery levels for most variables. Taking the anticipation and speech response together, a substantial cortisol response was observed for 55% of participants. The findings indicate that the Leiden Public Speaking Task might be particularly suited to investigate individual differences in sensitivity to social-evaluative situations.

  20. Antes da Queda do Muro, além dele e sem ele: A estética emergente em Die neuen Leiden...

    Directory of Open Access Journals (Sweden)

    Simone Malaguti

    2010-01-01

    Full Text Available Nesta análise do romance Die neuen Leiden des jungen W. (1973 em sua intertextualidade com os romances de Goethe, Defoe e Salinger, sugere-se que o texto de Plenzdorf cria um universo estilístico e ideológico instigante a uma produção inserida no contexto literário do Realismo Socialista dos anos 70 na Alemanha Oriental. Vinte anos após a Queda do Muro de Berlim, esse universo revela-se atual, senão modelar, para o (retrabalho de memória cultural da RDA representado na literatura e no cinema contemporâneos. Para tanto, recorre-se às análises da intertextualidade como um método de abordagem de processos de transformação. Expõem-se, então, os diferentes processos observados no romance em relação aos seus pré-textos.

  1. The Leiden/Argentine/Bonn (LAB) Survey of Galactic HI: Final data release of the combined LDS and IAR surveys with improved stray-radiation corrections

    CERN Document Server

    Kalberla, P M W; Hartmann, D; Arnal, E M; Bajaja, E; Morras, R; Poppel, W G L; Hartmann, Dap

    2005-01-01

    We present the final data release of observations of lambda 21-cm emission from Galactic neutral hydrogen over the entire sky, merging the Leiden/Dwingeloo Survey (LDS: Hartmann & Burton, 1997) of the sky north of delta = -30 deg with the Instituto Argentino de Radioastronomia Survey (IAR: Arnal et al., 2000, and Bajaja et al., 2005) of the sky south of delta = -25 deg. The angular resolution of the combined material is HPBW ~ 0.6 deg. The LSR velocity coverage spans the interval -450 km/s to +400 km/s, at a resolution of 1.3 km/s. The data were corrected for stray radiation at the Institute for Radioastronomy of the University of Bonn, refining the original correction applied to the LDS. The rms brightness-temperature noise of the merged database is 0.07 - 0.09 K. Residual errors in the profile wings due to defects in the correction for stray radiation are for most of the data below a level of 20 - 40 mK. It would be necessary to construct a telescope with a main beam efficiency of eta_{MB} > 99% to achi...

  2. Las plantas vasculares de la Península Ibérica en la obra de Clusio: envíos de semillas de Sevilla a Leiden

    Directory of Open Access Journals (Sweden)

    Menéndez de Luarca, Luis Ramón-Laca

    1997-12-01

    Full Text Available Before 1575 Carolus Clusius visited Spain and Portugal, in what can be considered the first extensive botanical collection trip for the Iberian Peninsula. He published in 1576 Rariorum aliquot stirpium per Hispanias observatarum historia, mainly based on materials collected during that expedition. The content of this book was later merged with additional information to créate a more comprehensive work dealing with the whole European flora, Rariorum plantarum historiae, published in 1601. An important element in the generation of this second book was the collaboration of Simón de Tovar, from Seville, who contributed with shipments of seeds and bulbs to Clusius. Thanks to Tovar, the number of plants recorded in Rar. stirp. hispan, hist, was increased with some remarkable examples in Rar. pl. hist. It is particularly interesting to note that at that moment American plants were a great novelty and Seville was the gate for the introduction of most of them, i.e. Sprekelia formosissima (L. Herbert [• Amaryllis formosissima L.] or Psidium guajava L. A reconstruction of Clusius itinerary based on the collection localities is given in this paper. A discussion of the correspondence between both botanists, including a critical list of the plants sent to Leiden by Tovar is presented.Carolus Clusius, o sencillamente Clusio, visitó España y Portugal antes de 1575, en lo que puede considerarse la primera herborización sistemática de la Península Ibérica. En 1576 fue publicada en Amberes la obra Rariorum aliquot stirpium per Hispanias observatarum historia, basada fundamentalmente en el material colectado durante este viaje. Esta obra fue refundida posteriormente con información adicional en Rariorum plantarían historiae, publicada en 1601, que trataba de abarcar el conjunto de la flora europea. Un elemento importante en la génesis de esta segunda obra fue la colaboración del sevillano Simón de Tovar, quien contribuyó con envíos de semillas

  3. The association of factor V leiden and prothrombin gene mutation and placenta-mediated pregnancy complications: a systematic review and meta-analysis of prospective cohort studies.

    Directory of Open Access Journals (Sweden)

    Marc A Rodger

    2010-06-01

    Full Text Available BACKGROUND: Factor V Leiden (FVL and prothrombin gene mutation (PGM are common inherited thrombophilias. Retrospective studies variably suggest a link between maternal FVL/PGM and placenta-mediated pregnancy complications including pregnancy loss, small for gestational age, pre-eclampsia and placental abruption. Prospective cohort studies provide a superior methodologic design but require larger sample sizes to detect important effects. We undertook a systematic review and a meta-analysis of prospective cohort studies to estimate the association of maternal FVL or PGM carrier status and placenta-mediated pregnancy complications. METHODS AND FINDINGS: A comprehensive search strategy was run in Medline and Embase. Inclusion criteria were: (1 prospective cohort design; (2 clearly defined outcomes including one of the following: pregnancy loss, small for gestational age, pre-eclampsia or placental abruption; (3 maternal FVL or PGM carrier status; (4 sufficient data for calculation of odds ratios (ORs. We identified 322 titles, reviewed 30 articles for inclusion and exclusion criteria, and included ten studies in the meta-analysis. The odds of pregnancy loss in women with FVL (absolute risk 4.2% was 52% higher (OR = 1.52, 95% confidence interval [CI] 1.06-2.19 as compared with women without FVL (absolute risk 3.2%. There was no significant association between FVL and pre-eclampsia (OR = 1.23, 95% CI 0.89-1.70 or between FVL and SGA (OR = 1.0, 95% CI 0.80-1.25. PGM was not associated with pre-eclampsia (OR = 1.25, 95% CI 0.79-1.99 or SGA (OR 1.25, 95% CI 0.92-1.70. CONCLUSIONS: Women with FVL appear to be at a small absolute increased risk of late pregnancy loss. Women with FVL and PGM appear not to be at increased risk of pre-eclampsia or birth of SGA infants. Please see later in the article for the Editors' Summary.

  4. Predictive value of a profile of routine blood measurements on mortality in older persons in the general population: the Leiden 85-plus Study.

    Directory of Open Access Journals (Sweden)

    Anne H van Houwelingen

    Full Text Available BACKGROUND: Various questionnaires and performance tests predict mortality in older people. However, most are heterogeneous, laborious and a validated consensus index is not available yet. Since most older people are regularly monitored by laboratory tests, we compared the predictive value of a profile of seven routine laboratory measurements on mortality in older persons in the general population with other predictors of mortality; gait speed and disability in instrumental activities of daily living (IADL. METHODOLOGY/PRINCIPAL FINDINGS: Within the Leiden 85-plus Study, a prospective population-based study, we followed 562 participants aged 85 years for mortality over five years. At baseline (age 85 years high-density lipoprotein cholesterol, albumin, alanine transaminase, hemoglobin, creatinin clearance, C-reactive protein and homocysteine were measured. Participants were stratified based on their number of laboratory abnormalities (0, 1, 2-4 and 5-7. The predictive capacity was compared with gait speed (6-meter walking test and disability in IADL (Groningen Activity Restriction Scale by C-statistics. At baseline, 418 (74% 85-year old participants had at least one laboratory abnormality. All cause mortality risk increased with increasing number of laboratory abnormalities to a hazard ratio of 5.64 [95% CI 3.49-9.12] for those with 5-7 laboratory abnormalities (p<0.001 compared to those without abnormalities. The c-statistic was 0.66 [95% CI 0.59-0.69], similar to that of gait speed and disability in IADL. CONCLUSIONS/SIGNIFICANCE: In the general population of oldest old, the number of abnormalities in seven routine laboratory measurements predicts five-year mortality as accurately as gait speed and IADL disability.

  5. Thrombophilic Genetic Factors PAI-1, MTHFRC677T, V Leiden 506Q, and Prothrombin 20210A in Noncirrhotic Portal Vein Thrombosis and Budd-Chiari Syndrome in a Caucasian Population

    Directory of Open Access Journals (Sweden)

    Mario D’Amico

    2013-01-01

    Full Text Available Thrombophilic genetic factors PAI-1, MTHFRC677T, V Leiden 506Q, and Prothrombin 20210A were studied as risk factors in 235 Caucasian subjects: 85 patients with abdominal thrombosis (54 with portal vein thrombosis (PVT and 31 with Budd-Chiari syndrome (BCS without liver cirrhosis or hepatocellular carcinoma and 150 blood bank donors. Seventy-five patients with PVT/BCS showed associated disease or particular clinical status (46 PVT/29 BCS: 37 myeloproliferative neoplasm (20 PVT/17 BCS, 12 abdominal surgery (10 PVT/2 BCS, 10 contraception or pregnancy (6 PVT/4 BCS, 7 abdominal acute disease (6 PVT/1 BCS, and 9 chronic disease (4 PVT/5 BCS; ten patients did not present any association (8 PVT/2 BCS. PAI-14G-4G, MTHFR677TT, and V Leiden 506Q were significantly frequent (OR 95% CI and χ2 test with P value in abdominal thrombosis; in these patients PAI-14G-4G and MTHFR677TT distributions deviated from that expected from a population in the Hardy-Weinberg equilibrium (PAI-1: χ2=13.8, P<0.001; MTHFR677: χ2=7.1, P<0.01, whereas the equilibrium was respected in healthy controls. V Leiden Q506 and Prothrombin 20210A were in the Hardy-Weinberg equilibrium both in patients with abdominal thrombosis and healthy controls. Our study shows an important role of PAI-14G-4G and MTHFR677TT in abdominal thrombosis without liver cirrhosis or hepatocellular carcinoma.

  6. Factor V Leiden mutation and high FVIII are associated with an increased risk of VTE in women with breast cancer during adjuvant tamoxifen - results from a prospective, single center, case control study.

    Science.gov (United States)

    Kovac, Mirjana; Kovac, Zeljko; Tomasevic, Zorica; Vucicevic, Slavko; Djordjevic, Valentina; Pruner, Iva; Radojkovic, Dragica

    2015-01-01

    Estimates of the risk ratio of tamoxifen-associated venous thromboembolism (VTE) in breast cancer patients range from 2.4 to 7.1. The occurrence of thrombosis in patients with breast cancer complicates the clinical condition and causes a change of treatment. Our study was conducted in order to investigate the influence of patient-related risk factors for thrombosis development in breast cancer patients whose treatment included adjuvant tamoxifen. The prospective, single center, case control study included 150 breast cancer women, 50 whom developed venous thrombosis during adjuvant tamoxifen and 100 whom did not have thrombosis, as a control group. Patient-related risk factors such as: age, body mass index, previous VTE, varicose veins, concomitant diseases, the presence of prothrombotic mutations (FV Leiden, FII G20210A) and FVIII activity were evaluated in both groups. In respect of prothrombotic mutations, the FV Leiden mutation was present in a higher number of women from the VTE group (10/50 vs 7/100; P=0.020). Additionally, FVIII activity was significantly higher in the VTE group; median (IQR), of 1.79 (0.69) vs 1.45 (0.55); P1.5IU/ml, who were carriers of prothrombotic mutations, an OR of 3.76 (CI 95% 1.276-11.096; P=0.016) was obtained for VTE. The results of our study showed that the factor V Leiden mutation and high FVIII are associated with an increased risk of VTE in women with breast cancer during adjuvant tamoxifen. Copyright © 2014. Published by Elsevier B.V.

  7. DANCERS: Delft advanced news retrieval system

    Science.gov (United States)

    Hanjalic, Alan; Kakes, Geerd; Lagendijk, Reginald L.; Biemond, Jan

    2001-01-01

    In this paper we present a system for automated analysis, classification and indexing of broadcast news programs. The system first analyses the visual and the speech stream of an input news program in order to obtain an initial partitioning of the program into the so-called report segments. The analysis of the visual stream provides the boundaries of the report segments lying at the beginning and the end of each anchorperson shot. This analysis step is performed by applying an existing techniques for anchorperson shot detection. The analysis of the speech stream gives the boundaries of the report segments lying in the middle of each silent interval. Then, the transcribed speech of each of the report segments is matched with the content of a large pre-specified textual topic database. This database covers a large number of topics and can be updated by the user at any time. Fro each topic a vast number of keywords is given, each of which is also assigned a weight that indicates the importance of a keyword for a certain topic. The result of the matching procedure is a list of probable topics per report segment, where for each topic on the list a likelihood is computed based on the number of relevant keywords found in the segment and on the weights of those keywords. The list of topics per segment is then shortened by separating the most probable from least probable topics based on their likelihood. Finally, the likelihood values of the most probable topics are used in the last system module to merge related neighboring segments into reports. The most probable topics serving as the base for the segment-merging procedure are the same time the retrieval indexes for the reports and are used for classifying together all reports in the database that cover one and the same topic.

  8. Delft's solar car wins Solar Challenge 2003

    NARCIS (Netherlands)

    Van Kasteren, J.

    2003-01-01

    There were remarkable scenes in Adelaide, Australia,on the afternoon of Wednesday 22 October 2003 when a swathe of orange spilled through the city. Barely visible at the heart of this burst of colour was the Nuna II, a futuristic vehicle which had just won the Solar Challenge 2003, a four-day journe

  9. The factor V leiden , the G20210A mutation of prothrombin gene and their association with deep vein thrombophilia%凝血因子V基因Leiden 突变和凝血酶原基因G20210A突变与下肢深静脉血栓形成关系的探讨

    Institute of Scientific and Technical Information of China (English)

    郭辰虹; 郭琼行; 龚瑶琴; 陈丙玺; 常宏; 周海斌

    2002-01-01

    目的:探讨凝血因子V 基因G1691A突变(Leiden 突变)和凝血酶原基因G20210A突变与下肢深静脉血栓形成的关系.方法:采用PCR---RFLP技术对86例下肢深静脉血栓形成患者和100例正常对照的健康人群进行凝血因子 V 基因G1691A突变和凝血酶原基因G20210A突变检测.结果:86例下肢深静脉血栓形成患者和100例正常对照组中均未检出凝血因子V基因 G1691A突变和凝血酶原基因G20210A突变.结论:凝血因子V基因G1691A突变和凝血酶原基因G20210A突变可能不是中国汉族人群下肢深静脉血栓形成的主要风险因子.

  10. [Prevalence of thrombophilic mutations of FV Leiden, prothrombin G20210A and PAl-1 4G/5G and their combinations in a group of 1450 healthy middle-aged individuals in the Prague and Central Bohemian regions (results of FRET real-time PCR assay)].

    Science.gov (United States)

    Kvasnicka, Jan; Hájková, Jaroslava; Bobcíková, Petra; Kvasnicka, Tomás; Dusková, Daniela; Poletínová, Sárka; Kieferová, Veronika

    2012-01-01

    Factor V Leiden (G1691A) and prothrombin gene (FII G20210A) mutations are independent risk factors of venous thrombosis and this risk is further increased by the combined genotype 4G/4G PAI-1. The primary objective was to identify the frequency of mutations of minor alleles and genotypes of FVL, FII G20210A and PAI-1 4G/5G in healthy Caucasians in the Prague and Central Bohemia regions. The secondary objective was to identify the occurrence of their mutual combinations. Genotyping was performed in 1,450 healthy individuals (blood donors, 981 men and 469 women) using robotic DNA isolation and subsequent PCR and melting curve analysis (Light Cycler 480 System, Roche). The minor allele frequencies in FV Leiden and FII G20210A mutations were 4.5% and 1.3% respectively. The frequency of the 4G PAI-1 allele was 55.9%. The genotype frequencies were as follows: GG 91.10%, GA 8.83% and AA 0.07% for FV Leiden; GG 97.38%, GA 2.55% and AA 0.07% for FII G20210A and 4G/4G 30.69%, 4G/5G 50.34% and 5G/5G 18.97% for PAI-1. No differences in these frequencies were found between the genders. The occurrence of the combined heterozygous FII and heterozygous FV Leiden mutations was 0.14%. The PAI-1 4G/4G genotype was combined with the heterozygous FV leiden mutation in 2.83% of cases and with the heterozygous FII mutation in 0.62% of cases. The found frequencies of genotypes and alleles confirm a relatively high prevalence of hereditary thrombophilia in the Czech Republic.

  11. Avaliação da incidência das mutações G1691A no gene do fator V (fator V Leiden e G20210A no gene da protrombina em pacientes com diabetes mellitus tipo 2 Incidence of G1691A factor V gene (factor V Leiden and G20210A prothrombin gene mutations in type 2 diabetes patients

    Directory of Open Access Journals (Sweden)

    Anna L. Soares

    2005-09-01

    Full Text Available Em virtude da alta prevalência de Diabetes mellitus tipo 2 (DM2 na população mundial e da alta taxa de mortalidade decorrente de eventos trombóticos, é de extrema importância o conhecimento das alterações no sistema hemostático em pacientes portadores deste distúrbio. A mutação no gene do fator V (G1691A - fator V Leiden em heterozigose ou homozigose confere aos portadores o fenótipo de resistência à proteína C ativada, situação que aumenta em sete vezes o risco de desenvolver uma trombose. A mutação G20210A no gene da protrombina resulta no quadro de hiperprotrombinemia, aumentando o risco de trombose em três vezes. A pesquisa dessas mutações de interesse em trombofilia é de grande relevância considerando que a presença das mesmas pode exacerbar o estado de hipercoagulabilidade acelerando as complicações no diabetes. O presente estudo teve como objetivo avaliar a incidência dessas mutações em indivíduos hígidos (Controle, n=16, pacientes com DM2 (n=7, com hipertensão (HAS, n=12 e com DM2+HAS (n=18, através da técnica de PCR-RFLP. As freqüências encontradas nos grupos estudados foram baixas e similares àquelas observadas na população brasileira em geral. Não foi possível estabelecer correlação entre a presença da mutação e características específicas de cada grupo. Dessa forma, ainda não está claro se há ou não uma maior prevalência dessas mutações em indivíduos diabéticos e se a presença das mesmas contribui para o aumento do risco de desenvolver trombose nesses indivíduos, sendo necessário estudos mais amplos para a elucidação da questão.Because of the high prevalence of type 2 diabetes mellitus (DM2 worldwide and the high mortality rate due to thrombotic events, it is extremely important to know about changes in the hemostatic system of such patients. Factor V mutation (G1691A - factor V Leiden in either heterozygosis or homozygosis confers the activated protein C resistant

  12. Einige Ericaceen des Leidener Herbariums

    NARCIS (Netherlands)

    Smith, J.J.

    1916-01-01

    Frutex, ramulis validiusculis, rigidis, vix tortilibus, plus minusve obtuso-trigonis vel subteretibus, glabris. Folia alterna, breviter petiolata, oblongo-ovata, sensim acuminata, angusta obtuse callosoapiculata, basi rotundata et brevissime in petiolum contracta, densius crenato-serrata, dentibus c

  13. Agrarisch ondernemers leiden leerlingen op

    NARCIS (Netherlands)

    Noorduyn, L.

    2008-01-01

    Een boer of tuinder die een neventak heeft als onderwijs-leerbedrijf? In de toekomst is dat heel goed mogelijk, denkt Jan Pieter Janssen, voorzitter van het college van bestuur van het Citaverde College in Limburg. Het zou het ultieme vervolg zijn op de pilot “Bedrijf als schakelplaats in de groene

  14. Effect of Discontinuation of Antihypertensive Treatment in Elderly People on Cognitive Functioning--the DANTE Study Leiden: A Randomized Clinical Trial.

    Science.gov (United States)

    Moonen, Justine E F; Foster-Dingley, Jessica C; de Ruijter, Wouter; van der Grond, Jeroen; Bertens, Anne Suzanne; van Buchem, Mark A; Gussekloo, Jacobijn; Middelkoop, Huub A; Wermer, Marieke J H; Westendorp, Rudi G J; de Craen, Anton J M; van der Mast, Roos C

    2015-10-01

    Observational studies indicate that lower blood pressure (BP) increases risk for cognitive decline in elderly individuals. Older persons are at risk for impaired cerebral autoregulation; lowering their BP may compromise cerebral blood flow and cognitive function. To assess whether discontinuation of antihypertensive treatment in older persons with mild cognitive deficits improves cognitive, psychological, and general daily functioning. A community-based randomized clinical trial with a blinded outcome assessment at the 16-week follow-up was performed at 128 general practices in the Netherlands. A total of 385 participants 75 years or older with mild cognitive deficits (Mini-Mental State Examination score, 21-27) without serious cardiovascular disease who received antihypertensive treatment were enrolled in the Discontinuation of Antihypertensive Treatment in Elderly People (DANTE) Study Leiden from June 26, 2011, through August 23, 2013 (follow-up, December 16, 2013). Intention-to-treat analyses were performed from January 20 through April 11, 2014. Discontinuation (n=199) vs continuation (n=186) of antihypertensive treatment (allocation ratio, 1:1). Change in the overall cognition compound score. Secondary outcomes included changes in scores on cognitive domains, the Geriatric Depression Scale-15, Apathy Scale, Groningen Activity Restriction Scale (functional status), and Cantril Ladder (quality of life). Compared with 176 participants undergoing analysis in the control (continuation) group, 180 in the intervention (discontinuation) group had a greater increase (95% CI) in systolic BP (difference, 7.36 [3.02 to 11.69] mm Hg; P=.001) and diastolic BP (difference, 2.63 [0.34 to 4.93] mm Hg; P=.03). The intervention group did not differ from the control group in change (95% CI) in overall cognition compound score (0.01 [-0.14 to 0.16] vs -0.01 [-0.16 to 0.14]; difference, 0.02 [-0.19 to 0.23]; P=.84). The intervention and control groups did not differ significantly

  15. The frequency of β-globin gene haplotypes, α-thalassemia and genetic polymorphisms of methylenetetrahydrofolate reductase, factor V Leiden and prothrombin genes in children with sickle cell disease in Rio de Janeiro, Brazil Frequência dos haplótipos da globina beta, da talassemia alfa e dos polimorfismos genéticos dos genes da metilenotetrahidrofolato redutase, do fator V Leiden e da protrombina em crianças com doença falciforme no Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Isaac L. Silva Filho

    2010-02-01

    Full Text Available A freqüência dos haplótipos beta S e beta C do gene da globina e a prevalência de talassemia alfa e de mutações nos genes da metilenotetrahidrofolato redutase (MTHFR-C677T, do fator V de Leiden e da protrombina (G20210A foi estudada em crianças com doença falciforme do Rio de Janeiro. O haplótipo Bantu foi o mais freqüente (65,9%, 21,2% das crianças (18% heterozigotas e 3% homozigotas apresentam talassemia com mutação alfa 3.7kb, ao contrário da mutação alfa 4.2kb que não foi encontrada. Os alelos 677CT e 677TT da MTHFR foram observados em 20,2% e 4,8%, respectivamente. Os haplótipos Camarões, Árabe-Indiano e Senegal não foram detectados na amostra estudada, bem como mutações no gene do fator V de Leiden e da protrombina. Somente o haplótipo beta C CI foi observado. Esse é o primeiro estudo realizado em uma amostra proveniente do Programa de Triagem Neonatal para Hemoglobinopatias do estado do Rio de Janeiro. Apesar do Rio de Janeiro ser a segunda maior cidade brasileira e seus habitantes expressarem o elevado grau de miscigenação ocorrida no país, nossos resultados ainda coincidem com os registros históricos dos fluxos migratórios do gene beta S para o Brasil, bem como refletem a forte influência de indivíduos de origem africana na população do Rio de Janeiro.

  16. 维吾尔族静脉血栓栓塞症与凝血酶原G20210A和凝血因子V Leiden 突变的相关性研究%Association of polymorphism gene G20210A mutation or factor V Leiden mutation andactivated protein C resistance in Uyghur patients with Venous thromboembolism

    Institute of Scientific and Technical Information of China (English)

    胡雪梅; 艾力曼·马合木提; 夏玉宁; 古丽尼格尔·吾布力; 古丽米热·吐尼牙孜

    2015-01-01

    目的:探讨凝血酶原基因 G20210A(FⅡG20210A)和凝血因子 V基因 Leiden 突变(FVL)单核苷酸多态性(SNP)及活化蛋白C抵抗(APCR)与维吾尔族(维族)静脉血栓栓塞症(VTE)的关系。方法选择2008年1月-2013年6月在新疆医科大学第一附属医院住院的维族 VTE患者178例(病例组)及本院同期同民族住院体检者217例(健康对照组)。采用聚合酶链式反应-限制性片段长度多态性(PCR-PFLP)技术检测 FⅡG20210A 和FVL多态性,应用凝固法于SYSMEX CS-2000型全自动血液凝固仪检测APCR,并分析FⅡG20210A和FVL多态性及 APCR与 VTE的关联性。结果(1)所有受检对象均未检出 FⅡG20210A基因突变,其变异频率为0。(2)健康对照组中只有 GG基因型,而未见 GA和 AA基因型,A等位基因频率为0。病例组中 FVL多态性 GG型与GA/AA型分布频率差异有统计学意义(P =0.018),且A等位基因分布频率差异亦有统计学意义(P =0.018)。(3)病例组 APCR阳性率较健康对照组高(11.24%与4.60%),其差异有统计学意义(P <0.05)。但 VTE患者FVL多态性各基因型间 APCR阳性率比较差异无统计学意义(P >0.05)。(4)非条件 logistic回归分析后,肥胖(OR=1.599,95%CI 1.383~1.849,P =0.000)、高脂血症(OR =1.087,95%CI 1.035~1.141,P =0.001)和APCR阳性(OR =1.922,95%CI 1.880~5.561,P =0.033)是 VTE患者的独立危险因素。结论凝血酶原G20210A基因突变在新疆维族 VTE患者及正常人群中罕见甚至缺如。APCR及 FVL多态性是维吾尔族 VTE患者的危险因素,但 FVL多态性不是引起 APCR的主要因素。%Objective To investigate the association between the G20210A mutation of Prothrombin gene (FII G20210A),factor V Leiden mutation (FVL)and activated protein C resistance (APCR)in Uyghur patients with

  17. Bιβλιοκρισία του: V. SYROS, Die Rezeption der aristotelischen politischen Philosophie bei Marsilius von Padua. Eine Untersuchung zur ersten Diktion des Defensor pacis [Studies in Medieval and Reformation Traditions, ed. by A. C. Gow, v. 134], Leiden, 2007

    Directory of Open Access Journals (Sweden)

    Στυλιανός ΛΑΜΠΑΚΗΣ

    2012-01-01

    Full Text Available   Βιβλιοκρισία του : V. SYROS, Die Rezeption der aristotelischen politischen Philosophie bei Marsilius von Padua. Eine Untersuchung zur ersten Diktion des Defensor pacis. [Studies in Medieval and Reformation Traditions, edited by Andrew Colin Gow, volume 134], Brill, Leiden-Boston 2007, σελ. X+364, ISSN 1573-4188

  18. Dietary Antioxidants Decrease Serum Soluble Adhesion Molecule (sVCAM-1, sICAM-1 but not Chemokine (JE/MCP-1, KC Concentrations, and Reduce Atherosclerosis in C57BL but Not ApoE*3 Leiden Mice Fed an Atherogenic Diet

    Directory of Open Access Journals (Sweden)

    Nuala Murphy

    2005-01-01

    Full Text Available Dietary antioxidants are reported to suppress cellular expression of chemokines and adhesion molecules that recruit monocytes to the artery wall during atherosclerosis. In the present study we measured the effect of feeding apoE*3 Leiden mice or their non-transgenic (C57BL littermates with atherogenic diets either deficient in, or supplemented with, dietary antioxidants (vitamin E, vitamin C and β-carotene for 12 weeks, on serum levels of CC (JE/MCP-1 and CXC (KC chemokines and soluble adhesion molecules (sVCAM-1, sICAM-1 and atherosclerotic lesion size. ApoE*3 Leiden mice developed gross hypercholesterolaemia, and markedly accelerated (10–20 fold; P < 0.0001 atherogenesis, compared with non-transgenic animals. Antioxidant consumption reduced lesion area in non-transgenic, but not apoE*3 Leiden, mice. Serum sVCAM-1 and sICAM-1 levels were significantly (P < 0.0001 increased (sVCAM-1 up to 3.9 fold; sICAM-1 up to 2.4 fold by 4—8 weeks in all groups, and then declined. The initial increase in the concentration of adhesion molecules was reduced by 38%— 61% (P < 0.05 by antioxidant consumption, particularly in non-transgenic mice. By contrast, serum chemokine levels tended to increase more rapidly from baseline in apoE*3 Leiden mice, compared with non-transgenic animals, but were unaffected by dietary antioxidants. We conclude that dietary antioxidants reduce circulating soluble adhesion molecules and atherosclerosis in C57BL mice.

  19. PAI-1 4G-4G, MTHFR 677TT, V Leiden 506Q, and Prothrombin 20210A in Splanchnic Vein Thrombosis: Analysis of Individual Patient Data From Three Prospective Studies.

    Science.gov (United States)

    Pasta, Linda; Pasta, Francesca; D'Amico, Mario

    2016-03-01

    There are no univocal opinions on the role of genetic thrombophilia on splanchnic vein thrombosis (SVT). We defined genetic thrombophilia the presence of one of these thrombophilic genetic factors (THRGFs): PAI-1 4G-4G, MTHFR 677TT, V Leiden 506Q, and prothrombin 20210A. To evaluate the frequencies of these THRGFs in SVT patients, we analyzed individual data of 482 Caucasian patients, recruited from 2000 to 2014 in three prospective studies. SVT was defined as the presence of thrombosis of portal (PVT), mesenteric (MVT), splenic (SPVT), cava (CT), and hepatic vein (Budd Chiari syndrome, BCS). Pre-hepatic SVT (pre-HSVT) was defined as PVT with or without MVT/SPVT, without BCS. Post-hepatic SVT (post-HSVT) was BCS with or without PVT/MVT/SPVT. We compared 350 patients with liver cirrhosis (LC), 47 hepatocellular carcinoma (HCC), 37 myeloproliferative neoplasm (MPN), 38 associated disease (AD), 10 without any associated disease (WAD), vs 150 healthy controls (HC); 437 patients showed pre-HSVT and 45 post-HSVT. Thrombophilia was present in 294/482 (60.9%) patients: 189/350 LC (54.0%), 31/47 (66.0%) HCC, 29/39 (74.4%) MPN, 35/38 AD (92.1%), and 10/10 (100%) WAD, and 54/150 (36.0%) in HC. In the total group, we found 175 PAI-1 4G-4G, 130 MTHFR 677TT, 42V Leiden 506Q, and 27 prothrombin 20210A; 75 patients showed presence of >1 TRHGF; the more frequent association was PAI-1 4G-4G/MTHFR 677TT, in 36 patients. PAI-1 4G-4G and MTHFR 677TT were significantly more frequent in patients with SVT (P values 4G-4G and MTHFR 677TT distributions deviated significantly from that expected from a population in Hardy-Weinberg equilibrium. Thrombophilia was significantly less frequent in patients with pre-HSVT (250/437, 57.2%) than in patients with post-HSVT (44/45, 97.8%). Our study shows the significant prevalence of PAI-1 4G-4G and MTHFR 677TT in SVT, mainly in post-HSVT.

  20. LDE centres: sprint or marathon?

    NARCIS (Netherlands)

    Bonger, S.; Van Rein, E.

    2015-01-01

    The aim of the Strategic Leiden-Delft-Erasmus Alliance, established by the three universities in 2012, was to improve research and education and competitiveness. Projects are intended to develop from the ground up, which led to the establishment of eight joint centres in 2013. A quick look around re

  1. Potted history

    NARCIS (Netherlands)

    Van Dijk, T.

    2010-01-01

    The Jordan Valley was once populated by a people, now almost forgotten by historians, with whom the pharaoh of Egypt sought favour. That is the conclusion reached by Niels Groot, the first researcher to take a PhD at the Delft-Leiden Centre for Archaeology, Art History and Science.

  2. Lack of rivaroxaban influence on a prothrombinase-based assay for the detection of activated C protein resistance: an Italian ex vivo and in vitro study in normal subjects and factor V Leiden carriers.

    Science.gov (United States)

    Gessoni, G; Valverde, S; Valle, L; Gessoni, F; Caruso, P; Valle, R

    2017-08-01

    Activated protein C resistance (APCr) leads to hypercoagulability and is due, often but not exclusively, to Factor V Leiden (FVL). The aim of this study was to assess the ex vivo and in vitro interference of the direct factor Xa inhibitor rivaroxaban (RIV) on a prothrombinase-based assay for APCr detection. An ex vivo study was performed on fresh plasma samples obtained from 44 subjects with FV wild-type and seven with FVL heterozygous, all treated with RIV. An in vitro study was performed on 15 plasma samples (six from normal subjects, six from heterozygous, and three from homozygous FVL carriers, all frozen specimens) spiked with RIV. RIV concentration was evaluated using a chromogenic assay, and APCr was evaluated by a prothrombinase-based assay. No significant interference of RIV on APCr results obtained by a prothrombinase-based assay was observed for drug concentrations up to 400 ng/mL in FV wild-type and FVL carriers (homozygous and heterozygous). These results were confirmed both ex vivo and in vitro. RIV did not significantly interfere with the prothrombinase-based assay used for the assessment of APCr, and this was observed to occur independently of FV status. However, only concentrations up to 400 ng/mL were tested and, therefore, what occurs in the presence of higher doses remains to be investigated. © 2017 John Wiley & Sons Ltd.

  3. Different risk of deep vein thrombosis and pulmonary embolism in carriers with factor V Leiden compared with non-carriers, but not in other thrombophilic defects. Results from a large retrospective family cohort study

    Science.gov (United States)

    Mäkelburg, Anja B.U.; Veeger, Nic J.G.M.; Middeldorp, Saskia; Hamulyák, Karly; Prins, Martin H.; Büller, Harry R.; Lijfering, Willem M.

    2010-01-01

    The term factor V Leiden (FVL) paradox is used to describe the different risk of deep vein thrombosis and pulmonary embolism that has been found in carriers of FVL. In a thrombophilic family-cohort, we estimated differences in absolute risks of deep vein thrombosis and pulmonary embolism for various thrombophilic defects. Of 2,054 relatives, 1,131 were female, 41 had pulmonary embolism and 126 deep vein thrombosis. Annual incidence for deep vein thrombosis in non-carriers of FVL was 0.19% (95%CI, 0.16–0.23), and 0.41% (95%CI, 0.28–0.58) in carriers; relative risk (RR) 2.1 (95%CI, 1.4–3.2). For pulmonary embolism these incidences were similar in carriers and non-carriers 0.07%, respectively; RR 1.0 (95% CI, 0.4–2.5). When co-inheritance of other thrombophilic defects was excluded the RR for deep vein thrombosis in FVL carriers was 7.0 (95%CI, 2.3–21.7) compared to non-carriers and 2.8 (95%CI, 0.5–14.4) for pulmonary embolism. For other thrombophilic defects no such effect was observed. Thus the FVL paradox was confirmed in our study. However, a similar paradox in carriers of other thrombophilic defects was not observed. PMID:20007142

  4. Effects of probiotic Lactobacillus rhamnosus GG and Propionibacterium freudenreichii ssp. shermanii JS supplementation on intestinal and systemic markers of inflammation in ApoE*3Leiden mice consuming a high-fat diet.

    Science.gov (United States)

    Oksaharju, Anna; Kooistra, Teake; Kleemann, Robert; van Duyvenvoorde, Wim; Miettinen, Minja; Lappalainen, Jani; Lindstedt, Ken A; Kovanen, Petri T; Korpela, Riitta; Kekkonen, Riina A

    2013-07-14

    A high-fat diet disturbs the composition and function of the gut microbiota and generates local gut-associated and also systemic responses. Intestinal mast cells, for their part, secrete mediators which play a role in the orchestration of physiological and immunological functions of the intestine. Probiotic bacteria, again, help to maintain the homeostasis of the gut microbiota by protecting the gut epithelium and regulating the local immune system. In the present study, we explored the effects of two probiotic bacteria, Lactobacillus rhamnosus GG (GG) and Propionibacterium freudenreichii spp. shermanii JS (PJS), on high fat-fed ApoE*3Leiden mice by estimating the mast cell numbers and the immunoreactivity of TNF-α and IL-10 in the intestine, as well as plasma levels of several markers of inflammation and parameters of lipid metabolism. We found that mice that received GG and PJS exhibited significantly lower numbers of intestinal mast cells compared with control mice. PJS lowered intestinal immunoreactivity of TNF-α, while GG increased intestinal IL-10. PJS was also observed to lower the plasma levels of markers of inflammation including vascular cell adhesion molecule 1, and also the amount of gonadal adipose tissue. GG lowered alanine aminotransferase, a marker of hepatocellular activation. Collectively, these data demonstrate that probiotic GG and PJS tend to down-regulate both intestinal and systemic pro-inflammatory changes induced by a high-fat diet in this humanised mouse model.

  5. Late failure of a split-thickness skin graft in the setting of homozygous factor V Leiden mutation: a case report and correlative animal model from the Wound Etiology and Healing (WE-HEAL) study.

    Science.gov (United States)

    Shanmugam, Victoria K; McNish, Sean; Duncan, Joanna; Root, Brandy; Tassi, Elena; Wellstein, Anton; Kallakury, Bhaskar; Attinger, Christopher E

    2015-10-01

    We present the case of a 53-year-old Caucasian male smoker with remote history of left lower extremity deep venous thrombosis (DVT) and a strong family history of thrombosis, who presented to the Center for Wound Healing at MedStar Georgetown University Hospital with spontaneous left leg ulceration. Prothrombotic evaluation showed homozygosity for the factor V Leiden (FVL) mutation. Therapeutic anticoagulation was commenced with warfarin (Coumadin®) and the patient underwent successful debridement and Apligraf® followed by split-thickness skin graft (STSG) of two wounds. He had an uneventful postoperative course and on the 27th postoperative day the grafts were 95% intact. However, by postoperative day 41 there was 10% graft loss, and over the subsequent 2 weeks both grafts necrosed. On further questioning, it transpired that the patient had discontinued his warfarin on postoperative day 37 because he thought that it was no longer necessary. The patient is enrolled in the Wound Etiology and Healing (WE-HEAL) study, and at the time of the original graft, residual skin fragments from the STSG were transplanted onto a nude mouse for development of an animal model of wound healing. The mouse graft was successful and was harvested at postoperative day 87 for pathological examination. We review the mechanisms by which prothrombotic states, particularly FVL mutation, can contribute to skin graft failure and delayed wound healing. This case highlights the importance of considering prothrombotic conditions in patients with spontaneous leg ulcerations and the impact of therapeutic anticoagulation on healing. It further allows us to demonstrate the efficacy of the animal model in which residual fragments of STSG tissue are utilised for transplant onto nude mice for manipulation in the laboratory. © 2013 The Authors. International Wound Journal © 2013 Medicalhelplines.com Inc and John Wiley & Sons Ltd.

  6. The 2010 ACR/EULAR criteria are not sufficiently accurate in the early identification of autoantibody-negative rheumatoid arthritis: Results from the Leiden-EAC and ESPOIR cohorts.

    Science.gov (United States)

    Boeters, Debbie M; Gaujoux-Viala, Cécile; Constantin, Arnaud; van der Helm-van Mil, Annette H M

    2017-10-01

    The 2010 ACR/EULAR criteria were derived to classify rheumatoid arthritis (RA) earlier in time. Previous studies indeed observed that the 2010 criteria were fulfilled earlier than the 1987 criteria. This study determined whether the 2010 criteria perform equally in early classification of autoantibody-positive and autoantibody-negative RA. From the total Leiden-EAC (n = 3448) and ESPOIR (n = 813) RA patients who fulfilled the 1987 RA criteria at 1 year but not at presentation were selected (n = 463 and n = 53, respectively), as these patients were classified with delay with the 1987 criteria. These RA patients were studied on fulfilling the 2010 criteria at baseline (as 2010 positivity indicated that these RA patients were earlier identified) and these analyses were stratified for patients with and without anti-citrullinated protein antibodies (ACPA) and rheumatoid factor (RF). Analyses were repeated for DMARD start within the first year as reference for RA (instead of fulfilling the 1987 criteria). In the EAC, 75% of the selected RA patients did already fulfill the 2010 criteria at baseline. In ESPOIR this was 57%, indeed demonstrating early classification with the 2010 criteria. Among the selected autoantibody-positive RA patients of the EAC, 93% was already identified at baseline with the 2010 criteria. Within autoantibody-negative RA this was 51% (p < 0.001), indicating that 49% of autoantibody-negative RA patients were not early classified with the 2010 criteria. Similarly, within autoantibody-positive RA patients in ESPOIR 92% were 2010 positive at baseline, whereas this was only 25% within autoantibody-negative RA (p < 0.001), indicating that 75% of autoantibody-negative RA patients were not early classified with the 2010 criteria. Similar results were obtained when DMARD start was the reference for RA. The 2010 criteria perform well in the early identification of autoantibody-positive RA, but autoantibody-negative RA patients are still frequently missed

  7. Risk of venous thromboembolism associated with single and combined effects of Factor V Leiden, Prothrombin 20210A and Methylenetethraydrofolate reductase C677T: a meta-analysis involving over 11,000 cases and 21,000 controls

    Science.gov (United States)

    Simone, B; De Stefano, V; Leoncini, E; Zacho, J; Martinelli, I; Emmerich, J; Rossi, E; Folsom, AR; Almawi, WY; Scarabin, PY; den Heijer, M; Cushman, M; Penco, S; Vaya, A; Angchaisuksiri, P; Okumus, G; Gemmati, D; Cima, S; Akar, N; Oguzulgen, KI; Ducros, V; Lichy, C; Fernandez-Miranda, C; Szczeklik, A; Nieto, JA; Torres, JD; Le Cam-Duchez, V; Ivanov, P; Cantu, C; Shmeleva, VM; Stegnar, M; Ogunyemi, D; Eid, SS; Nicolotti, N; De Feo, E; Ricciardi, W; Boccia, S

    2014-01-01

    BACKGROUND Genetic and environmental factors interact in determining the risk of venous thromboembolism (VTE). The risk associated with the polymorphic variants G1691A of factor V (Factor V Leiden,FVL), G20210A of prothrombin (PT20210A) and C677T of methylentetrahydrofolate reductase (C677T MTHFR) genes has been investigated in many studies. METHODS We performed a pooled analysis of case-control and cohort studies investigating in adults the association between each variant and VTE, published on Pubmed, Embase or Google through January 2010. Authors of eligible papers, were invited to provide all available individual data for the pooling. The Odds Ratio (OR) for first VTE associated with each variant, individually and combined with the others, were calculated with a random effect model, in heterozygotes and homozygotes (dominant model for FVL and PT20210A; recessive for C677T MTHFR). RESULTS We analysed 31 databases, including 11,239 cases and 21,521 controls. No significant association with VTE was found for homozygous C677T MTHFR (OR: 1.38; 95% confidence intervals [CI]: 0.98–1.93), whereas the risk was increased in carriers of either heterozygous FVL or PT20210 (OR=4.22; 95% CI: 3.35–5.32; and OR=2.79;95% CI: 2.25–3.46, respectively), in double hterozygotes (OR=3.42; 95%CI 1.64-7.13), and in homozygous FVL or PT20210A (OR=11.45; 95%CI: 6.79-19.29; and OR: 2.79; 95%CI: 2.25 – 3.46, respectively). The stratified analyses showed a stronger effect of FVL on individuals ≤45 years (p-value for interaction = 0.036) and of PT20210A in women using oral contraceptives (p-value for interaction = 0.045). CONCLUSIONS In this large pooled analysis, inclusive of large studies like MEGA, no effect was found for C677T MTHFR on VTE; FVL and PT20210A were confirmed to be moderate risk factors. Notably, double carriers of the two genetic variants produced an impact on VTE risk significantly increased but weaker than previously thought. PMID:23900608

  8. Risk of venous thromboembolism associated with single and combined effects of Factor V Leiden, Prothrombin 20210A and Methylenetethraydrofolate reductase C677T: a meta-analysis involving over 11,000 cases and 21,000 controls.

    Science.gov (United States)

    Simone, Benedetto; De Stefano, Valerio; Leoncini, Emanuele; Zacho, Jeppe; Martinelli, Ida; Emmerich, Joseph; Rossi, Elena; Folsom, Aaron R; Almawi, Wassim Y; Scarabin, Pierre Y; den Heijer, Martin; Cushman, Mary; Penco, Silvana; Vaya, Amparo; Angchaisuksiri, Pantep; Okumus, Gulfer; Gemmati, Donato; Cima, Simona; Akar, Nejat; Oguzulgen, Kivilcim I; Ducros, Véronique; Lichy, Christoph; Fernandez-Miranda, Consuelo; Szczeklik, Andrzej; Nieto, José A; Torres, Jose Domingo; Le Cam-Duchez, Véronique; Ivanov, Petar; Cantu-Brito, Carlos; Shmeleva, Veronika M; Stegnar, Mojka; Ogunyemi, Dotun; Eid, Suhair S; Nicolotti, Nicola; De Feo, Emma; Ricciardi, Walter; Boccia, Stefania

    2013-08-01

    Genetic and environmental factors interact in determining the risk of venous thromboembolism (VTE). The risk associated with the polymorphic variants G1691A of factor V (Factor V Leiden, FVL), G20210A of prothrombin (PT20210A) and C677T of methylentetrahydrofolate reductase (C677T MTHFR) genes has been investigated in many studies. We performed a pooled analysis of case-control and cohort studies investigating in adults the association between each variant and VTE, published on Pubmed, Embase or Google through January 2010. Authors of eligible papers, were invited to provide all available individual data for the pooling. The Odds Ratio (OR) for first VTE associated with each variant, individually and combined with the others, were calculated with a random effect model, in heterozygotes and homozygotes (dominant model for FVL and PT20210A; recessive for C677T MTHFR). We analysed 31 databases, including 11,239 cases and 21,521 controls. No significant association with VTE was found for homozygous C677T MTHFR (OR: 1.38; 95 % confidence intervals [CI]: 0.98-1.93), whereas the risk was increased in carriers of either heterozygous FVL or PT20210 (OR = 4.22; 95 % CI: 3.35-5.32; and OR = 2.79;95 % CI: 2.25-3.46, respectively), in double heterozygotes (OR = 3.42; 95 %CI 1.64-7.13), and in homozygous FVL or PT20210A (OR = 11.45; 95 %CI: 6.79-19.29; and OR: 6.74 (CI 95 % 2.19-20.72), respectively). The stratified analyses showed a stronger effect of FVL on individuals ≤ 45 years (p value for interaction = 0.036) and of PT20210A in women using oral contraceptives (p-value for interaction = 0.045). In this large pooled analysis, inclusive of large studies like MEGA, no effect was found for C677T MTHFR on VTE; FVL and PT20210A were confirmed to be moderate risk factors. Notably, double carriers of the two genetic variants produced an impact on VTE risk significantly increased but weaker than previously thought.

  9. DUNBID, the Delft University neutron backscattering imaging detector

    Energy Technology Data Exchange (ETDEWEB)

    Bom, V.R. [Delft University of Technology, Mekelweg 15, 2629 JB Delft (Netherlands)]. E-mail: vb@iri.tudelft.nl; Eijk, C.W.E. van [Delft University of Technology, Mekelweg 15, 2629 JB Delft (Netherlands); Ali, M.A. [Atomic Energy Authority, Nuclear Research Center, Reactor and Neutron Physics Department, P.O. Box 13759, Abu Zabal, Cairo (Egypt)

    2005-12-01

    In the search for low-metallic land mines, the neutron backscattering technique may be applied if the soil is sufficiently dry. An advantage of this method is the speed of detection: the scanning speed may be made comparable to that of a metal detector. A two-dimensional position sensitive detector is tested to obtain an image of the back scattered thermal neutron radiation. Results of experiments using a radionuclide neutron source are presented. The on-mine to no-mine signal ratio can be improved by the application of a window on the neutron time-of-flight. Results using a pulsed neutron generator are also presented.

  10. The Delft Sand, Clay and Rock Cutting Model

    NARCIS (Netherlands)

    Miedema, S.A.

    2014-01-01

    Sand, clay and rock have to be excavated for a variety of purposes, such as dredging, trenching, mining (including deep sea mining), drilling, tunnel boring and many other applications. Many excavations take place on dry land, but they are also frequently required in completely saturated conditions,

  11. Ülikooli raamatukogu, Delft (Holland) / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    1999-01-01

    Hollandi arhitektuuribüroos Mecanoo projekteeritud, 1997. a. valminud Delfti Tehnikaülikooli raamatukoguhoone on maastikkusulanduva arhitektuuri näide. Klaasfassaadiga raamatukogu on pealt kaetud muruga. Efektne valge koonus sisaldab ülestikku ringikujulisi lugemistubasid, mis valguse saavad ülalt. Ülejäänud maja moodustab avatud saali, põhifond asub maa all.

  12. Delft Delta Design: The Houston Galveston Bay Region, Texas, USA

    NARCIS (Netherlands)

    Kothuis, B.L.M.; Brand, A.D.; Sebastian, A.G.; Nillesen, A.L.; Jonkman, S.N.

    2015-01-01

    In 2008, Hurricane Ike devastated Bolivar Peninsula, narrowly missing the more heavily industrialized and populated areas in the region. In the aftermath of the hurricane, the Severe Storm Prediction, Education and Evacuation from Disasters (SSPEED) Center at Rice University in Houston, and Texas A&

  13. Bouwmaterialen paalmatrassystemen: Delft Cluster Blijvend Vlakke Wegen, WP3

    NARCIS (Netherlands)

    Van der Meij, R.; Van Ruijven, J.

    2007-01-01

    Dit rapport betreft een literatuurstudie naar bouwmaterialen die in paalmatrassystemen worden gebruikt. De volgende aspecten komen aan bod: • Materialen voor paalmatrassystemen (palen, geotextielen, matrasmateriaal) • Hiaten in kennis (op basis van interviews) Door middel van deze literatuurstudie e

  14. Changing culture - the experience of TU Delft Library

    NARCIS (Netherlands)

    Heijne, M.; Van der Sar, E.

    2004-01-01

    When seeking to introduce change into an organisation, it is usually the organisational structure that is the main focus of attention. A reorganisation, however, does not necessarily resolve underlying cultural problems. It can, in fact, be just these cultural problems that prevent the organisation

  15. Advanced tyre modelling in DADS using DELFT-TYRE

    NARCIS (Netherlands)

    Oosten, J.J.M. van

    1999-01-01

    In vehicle dynamics studies detailed knowledge about the interaction forces between tyre and road are inevitable. Since the 1st publication by Pacejka of the Magic Formula Tyre model, the use of this model has become very popular, due to its accuracy, calculation speed and physical relations to the

  16. TU Delft y Educación Abierta

    OpenAIRE

    Mulder, Anka

    2015-01-01

    Jornada de Educación Abierta celebrada el 12 de marzo de 2015 en la Universidad Carlos III de Madrid en el marco de la Open Education Week promovida por el Consorcio OpenCourseWare 27 sliders / transparencias

  17. Neue Lycaenidenformen aus dem Rijksmuseum in Leiden

    NARCIS (Netherlands)

    Fruhstorfer, H.

    1915-01-01

    Lampides euchylas poliämus subspec. nova. ♂ Nur unwesentlich vom L. euchylas umbriel Fruhst. ♂ von Waigeoe differenziert, ♀ dagegen mit allen Kennzeichen einer Satellitinselrasse versehen. Flügel oberseits breiter schwarz umrahmt, so dass der weisse Diskalfleck der Vorderflügel reduziert wird. Der b

  18. Liebe ohne Leiden? Love without Sorrows?

    Directory of Open Access Journals (Sweden)

    Stefan Müller

    2008-11-01

    Full Text Available Im vorliegenden Sammelband werden unterschiedliche Zugänge und Herangehensweisen zum Thema ‚Liebe im Kapitalismus‘ nebeneinandergestellt. Die Idee der Liebe innerhalb der ‚Logik des Kapitalismus‘ soll genauer untersucht werden. Dafür werden Erfahrungen und Reflexionen aus Kunst, Literatur, Philosophie und Psychoanalyse sowie aus Datenerhebungen und Interviews herangezogen und analysiert. Die Beiträge gruppieren sich zumeist allerdings stärker um das Themenfeld der Liebe. Veränderungen der kapitalistischen Logik nachzuzeichnen, wird in den Ausführungen kaum angestrebt.The volume at hand compares varying approaches to the theme of ‘love under capitalism.’ The intention is to more closely examine the idea of love within the ‘logic of capitalism.’ In order to achieve this, experiences and reflections from art, literature, philosophy, and psychoanalysis as well as data collections and interviews are drawn out and analyzed. The contributions, however, are mostly grouped around the thematic of love. Most of the studies barely begin to sketch the changes of capitalist logic.

  19. Association of prothrombin G20210A and coagulation factor Leiden gene mutation with coronary heart disease%凝血酶原基因G20210A和凝血因子V Leiden突变与冠心病的相关性探讨

    Institute of Scientific and Technical Information of China (English)

    金国栋; 徐耕; 傅国胜; 马骥; 施育平; 单江

    2003-01-01

    目的:探讨凝血酶原基因G20210A和凝血因子VG1691A(Leiden)突变在中国汉族人群中的发生率及与冠心病的关系.方法:用聚合酶链反应和限制性内切酶片段长度多态性技术,对234例冠心病患者和210名正常人的凝血酶原基因G20210A和凝血因子V Leiden突变进行分析.结果:找到了一例正常人的凝血酶原基因G20210A变异,突变率为0.2%.冠心病和正常人中均未发现凝血因子V Leiden突变.结论:汉族人群中存在凝血酶原基因G20210A突变,但凝血酶原基因G20210A和凝血因子V Leiden变异在我国的发生率较低,不足以作为冠心病的遗传性危险因素.

  20. Correlation with Platelet Parameters and Genetic Markers of Thrombophilia Panel (Factor II g.20210G>A, Factor V Leiden, MTHFR (C677T, A1298C), PAI-1, β-Fibrinogen, Factor XIIIA (V34L), Glycoprotein IIIa (L33P)) in Ischemic Strokes.

    Science.gov (United States)

    Tasdemir, Sener; Erdem, Haktan Bagis; Sahin, Ibrahim; Ozel, Lutfi; Ozdemir, Gokhan; Eroz, Recep; Tatar, Abdulgani

    2016-06-01

    An important type of arterial thrombosis, ischemic stroke is associated with increased mortality risk, severe disability and life quality impairment. In this study, we analyzed mean platelet volume, platelet count values and genetic thrombophilia markers of patients who have ischemic stroke history and searched the relationship with genetic predisposition of ischemic strokes and platelet parameters. A retrospective, clinical trial was performed by reviewing the ischemic stroke history (except cryptogenic events) of 599 patients and 100 controls. The results of the genetic thrombophilia panel were used to classify the study group and control group into low and high risk for thrombophilia groups. The high-risk group included patients homozygous/heterozygous for Factor II g.20210G>A or Factor V Leiden mutations with/without any other polymorphism. The low-risk group included patients heterozygous or homozygous for MTHFR (C677T, A1298C), PAI-1, β-fibrinogen, Factor XIIIA (V34L) and glycoprotein IIIa (L33P) polymorphisms or negative in terms of both mutations and polymorphisms. The results of study showed us that high-risk group mutations are important risk factors for ischemic stroke but low-risk group polymorphisms are not significant. According to platelet parameters, although there was a significant difference between MPV and PLT values of ischemic stroke and control group, thrombophilia mutations and polymorphisms have not a significant effect on MPV and PLT values in ischemic stroke patients.

  1. α-半乳糖苷酶A缺乏对凝血因子V Leiden小鼠组织纤维蛋白沉积和血栓形成的影响%Effect α-galaetosidase A deficiency on FV Leiden fibtin deposition and thrombosis in mice

    Institute of Scientific and Technical Information of China (English)

    沈粤春; 何兆初; 蔡茹丽; 潘洁贞; 王晓明; 李君

    2009-01-01

    Objective To evaluate the effect of q-galaetosidase A(Gla)deficiency on FV Leiden (FVL)associated thrombosis in vivo.Metlaods To generate the mice carrying mutations in Gla and F V L and analyze the tissue fibrin deposition in organs and thrombosis.Results in the presence of F V L,Gla deficiency greatly increased tissue fibrin deposition compared with that in wild-type[Gla-/0 FV Q/Q vs.Gla+/0 FV Q/Q=(0.24±0.07)% vs.(0.086±0.049)%,P<0.0001;Gla-/-FV Q/Q vs.Gla+/+ FV Q/Q=(0.32±0.03)% vs.(0.06±0.005)%,P<0.05].With Gla deficiency,the number of thrombi on organ sectiona in F V L mice was signifieantly increased[(Gla-/-FV Q/Q and Gla-/0 FV Q/Q) vs.(Gla+/+ FV Q/Q and Gla+/0 FV Q/Q)=1.9±0.7 vs.0.3±0.1,P<0.05].Conclusions Gla deficieney could be an important genetic modifier for the enhaneed thrombosis associated with F V L.%目的 评价α-半乳糖苷酶A(Gla)缺乏与凝血因子V(FV)Leiden(FVL)在体内对血栓形成的影响.方法 建立Gla与FVL基因复合突变小鼠,并分析其器官组织纤维蛋白沉积和血栓形成情况.结果 在FVL存在情况下,野生型小鼠组织器官纤维蛋白沉积[Gla+/0 FV Q/Q,(0.08±0.05)%]较Gla缺乏型[Gla-/0 FV Q/Q,(0.24±0.07)%)]显著降低(P<0.01),Gla缺乏型纯合子小鼠组织器官纤维蛋白沉积[Gla-/-FV Q/Q,(0.32±0.03)%)]较Gla野生型[Gla+/+FV Q/Q,(0.06±0.01)%)]显著增加(P<0.05);同时Gla缺乏型小鼠(Gla-/-FV Q/Q和Gla-/0 FV Q/Q)血栓数目[(1.9±0.7)个]亦显著高于Gla野生型小鼠(Gla+/+FV Q/Q和Gla+/0 FV Q/Q)的血栓数目[(0.3±0.1)个](P<0.05).结论 Gla缺乏加重FVL突变小鼠的组织纤维蛋白沉积和血栓形成,提示Cla缺乏可能是重要的增强FVL患者血栓形成的遗传因素,临床上FVL患者出现早发和严重的血栓疾病可能合并其他遗传性血栓相关的基因缺陷.

  2. Factor V Leiden is associated with increased sperm count

    DEFF Research Database (Denmark)

    van Mens, T E; Joensen, U N; Bochdanovits, Z

    2017-01-01

    ) of consecutively included men without known causes for spermatogenic failure, and performed an individual patient data meta-analysis of these two cohorts together with one previously published (Dutch, n = 908) cohort. We explored possible biological underpinnings for the relation between sperm count and FVL......, by use of a FVL-mouse model and investigations of genetic linkage. PARTICIPANTS/MATERIALS, SETTING, METHODS: Participants were male partners of subfertile couples (two Dutch cohorts) and young men from the general population (Danish cohort): FVL carrier rate was 4.0%, 4.6% and 7.3%, respectively...... FVL carriers have a higher total sperm count than non-carriers, with an adjusted mean difference of 31 × 106 (95%CI 0.2-61.7; P = 0.048). Mice with the FVL mutation do not have increased spermatogenesis as compared to wildtype mice. None of the studied polymorphisms was in linkage disequilibrium...

  3. Factor V Leiden Mutation and PT 20210 Mutation Test

    Science.gov (United States)

    ... is suspected that a person has an inherited risk factor for blood clots, for example, when an individual: Has a first deep venous thrombosis (DVT) or venous thromboembolism (VTE) before age 50 ...

  4. On Rhinoceros simus, Burchell in the Leiden Museum

    NARCIS (Netherlands)

    Jentink, F.A.

    1890-01-01

    According to Dr. P. L. Sclater (Nature, September 25, 1890, p. 520) Mr. F. Selous says in the Field of August 16 as follows: — »it was within a mile of this spot that, two years previously (i. e. in 1888), I shot two white Rhinoceroses Rhinoceros simus), the last of their kind that have been killed

  5. Beschreibung neuer Hymenophyllaceae aus dem Rijks Herbarium zu Leiden

    NARCIS (Netherlands)

    Rosenstock, E.

    1912-01-01

    Euhymenophyllum; rhizomate repente, filiformi, validiusculo, glabrescente; stipitibus subflexuosis, firmis, basin fere usque crispo-alatis, 2-3 cm. longis; laminis e basi parum angustata linearibus, obtusis, rigide membranaceis, fuscis, glaberrimis, subtripinnatifidis, 4½ cm. longis, 1½ cm. latis; s

  6. Integrative Biological Analysis of the APOE*3-Leiden Transgenic Mouse

    NARCIS (Netherlands)

    Clish, C.B.; Davidov, E.; Oresic, M.; Plasterer, T.N.; Lavine, G.; Londo, T.; Meys, M.; Snell, P.; Stochaj, W.; Adourian, A.; Zhang, X.; Morel, N.; Neumann, E.; Verheij, E.; Vogels, J.T.W.E.; Havekes, L.M.; Afeyan, N.; Regnier, F.; Greef, J. van der; Naylor, S.

    2004-01-01

    Integrative (or systems biology) is a new approach to analyzing biological entities as integrated systems of genetic, genomic, protein, metabolite, cellular, and pathway events that are in flux and interdependent. Here, we demonstrate the application of intregrative biological analysis to a mammalia

  7. Point processes in traffic safety analysis. Thesis Leiden.

    NARCIS (Netherlands)

    Bijleveld, F.D.

    1995-01-01

    This study investigates the possibilities for analysing accident data independently of the choice of the length of the intervals used, and consequently independently of the choice of the starting point of the sequence of accident counts. This seems to be possible using the original points of time as

  8. Factor V Leiden and post thromboembolic pulmonary hypertension.

    Science.gov (United States)

    Naudziūnas, Albinas; Miliauskas, Skaidrius

    2003-01-01

    Literature review and original data concerning the most common cause of inherited thrombophilia - activated protein C resistance have been presented. One hundred and three patients with confirmed venous thromboembolism have been investigated for activated protein C resistance with 2 ( nd ) generation "Diagnostica Stago" test. Activated protein C resistance has been found in 22.3 % cases. In the group of 70 healthy unselected men and women, matched by sex and age, this mutation has been found in 7.1% cases. Out of 101 patients with pulmonary thromboembolism confirmed by pulmoangiography or perfusion lung scan, 78 have been investigated by Doppler echocardioscopy in order to detect pulmonary hypertension after 1.5 months. Statistically significant correlation between age and pulmonary artery pressure has been found. No correlation between pulmonary artery pressure and activated protein C resistance has been detected.

  9. Greenhouse IPM in the University of Leiden's botanical garden.

    NARCIS (Netherlands)

    Lenteren, van J.C.; Kole, M.; Vliet, van G.J.C.M.

    1986-01-01

    An experiment is made with the conversion of chemical control to IPM. It is concluded that IPM is possible in greenhouses containing many plant species. However it is not easy to begin such programs. Intensive training and continuous guidance of greenhouse personnel is imperative

  10. Building the fastest bicycle in the world; a year with the Human Power Team Delft & Amsterdam

    NARCIS (Netherlands)

    Schuurman, M.; Roks, G.

    2014-01-01

    “Third time lucky” is the saying, but for the third Human Power Team it almost turned out as a year for nothing. The team has been attempting to set the world record for the fastest bicycle powered only by human muscles for two years already. This endeavor to let a Dutchman be the fastest human on e

  11. DELFT-TYRE: high frequency tyre modelling using SWIFT-Tyre

    NARCIS (Netherlands)

    Oosten, J.J.M. van; Jansen, S.T.H.

    1999-01-01

    As is well known, Magic Formula tyre modelling (MF-Tyre is a part of ADAMS/Tire) allows an accurate and efficient description of tyre-road interaction forces required for any usual vehicle handling simulation. When it comes to modelling of tyre behaviour at higher frequencies, which are important fo

  12. [Johannes Vermeer and Anthon van Leeuwenhoek: Delft Art and Science together during the golden Dutch century].

    Science.gov (United States)

    Miranda C, Marcelo

    2009-04-01

    Johannes Vermeer and Anthon van Leeuwenhoek are among the greatest geniuses in Art and Science respectively. During the seventeenth century, they achieved innovative advances. Vermeer, in painting, created a new intimate view of people specially women, developing the treatment of light and details. Leeuwenhoek, friend of Vermeer, influenced him in the use of the obscure camera in his works. In spite of having no formal academic education, he made extremely relevant discoveries with the use of microscope. He showed for first time human spermatozoids, red blood cells, brain, nerve and muscle structures and described many living animals. These two brilliant contemporary Dutch men made a great contribution to our civilization.

  13. Grondwatereffecten aan de oppervlakte (gebracht): Onderzoek naar effecten van stopzetting grondwaterontrekking DSM Delft - Technisch rapport

    NARCIS (Netherlands)

    Roelofsen, F.; Goorden, N.

    2008-01-01

    Deze rapportage bevat de beschrijving van de afzonderlijke modellen en is om die reden opgebouwd uit 5 delen. Deel I beschrijft de totstandkoming van de geo(hydro)logie van de ondergrond. Daarbij wordt onderscheid gemaakt tussen Pleistoceen en Holoceen waarbij het Holoceen zowel regionaal als ook in

  14. A safer road environment for cyclists. Proefschrift Technische Universiteit Delft TUD.

    NARCIS (Netherlands)

    Schepers, P.

    2013-01-01

    This thesis focuses on the question of how the road environment (road design and network characteristics) affects road safety for cyclists through effects on risk and exposure to risk. In this thesis, the term ‘road design’ is used to denote the location level (e.g. intersection design) while the te

  15. The multi-functional cleanroom of TNO Building and Construction Research at Delft (NL)

    NARCIS (Netherlands)

    Ham, P.J.

    1996-01-01

    For studies in rooms in which dust-free air is important, the department of Indoor Environment, Building Physics and Systems of TNO Building and Construction Research has access to a multifunctional cleanroom annex operating theatre (OP). On the ICCCS-symposium in Zürich 1990 TNO Building and Constr

  16. Riverine Flow Observations and Modeling: Sensitivity of Delft3D River Model to Bathymetric Variability

    Science.gov (United States)

    2011-09-30

    performed by a person walking with a GPS-equipped backpack and with an echosounder- equipped electric kayak . The meandering reach (Figure 2) is a deep (~10m...Structures in a Natural Gravel-Bed River submitted to Geophysical Research Letters. Manuscript Abstract Stream-wise, horizontal length scales and...Unmanned Surface Vehicle (USV), an Unmanned Underwater Vehicle (UUV), and some additional stationary platforms and were deployed in a number of natural

  17. IVERINE FLOW OBSERVATIONS AND MODELING: Sensitivity of Delft3D River Model to Bathymetric Variability

    Science.gov (United States)

    2011-01-01

    equipped backpack and with an echosounder-equipped electric kayak . The meandering reach (Figure 2) is a deep (~10m) channel with flows around 0.5m/s...Kootenai River, ID on 12-16 August 2010. The study reach contained a number of natural channel features, such as a pool-riffle sequence and bank...irregularities, which influence transverse mixing. The dye was released at a constant rate for one hour from a kayak fixed in the center of the channel

  18. Application of the Delft Numerical models in the Lower Yellow River

    NARCIS (Netherlands)

    Liu, H.L.; Liang, G.T.; Wu, S.Q.; Ji, Z.W.

    2002-01-01

    Because of heavy deposition and water shortage, the Yellow River faces the risk of flooding and no-flow since the middle of 1980s. The problem could be solved only by integrated water management. However, Dutch has a long history fighting the flood and gets a lot of experiences in water management,

  19. The status of the Delft University Neutron Backscatter Landmine Detector (DUNBLAD).

    Science.gov (United States)

    Bom, V R; Datema, C P; van Eijk, C W E

    2004-07-01

    The neutron backscattering technique may be applied to search for non-metallic landmines in relatively dry soils. A detector system using this technique has been constructed. Tests showed that anti-tank mines can reliably be found, but that, depending on the circumstances, anti-personnel mines may escape detection. A first test with a pulsed neutron generator shows that further improvements can be achieved by applying a window on the neutron transit time.

  20. The status of the Delft University Neutron Backscatter Landmine Detector (DUNBLAD)

    Energy Technology Data Exchange (ETDEWEB)

    Bom, V.R. E-mail: vb@iri.tudelft.nl; Datema, C.P. E-mail: cor.datema@philips.com; Eijk, C.W.E. van E-mail: vaneijk@iri.tudelft.nl

    2004-07-01

    The neutron backscattering technique may be applied to search for non-metallic landmines in relatively dry soils. A detector system using this technique has been constructed. Tests showed that anti-tank mines can reliably be found, but that, depending on the circumstances, anti-personnel mines may escape detection. A first test with a pulsed neutron generator shows that further improvements can be achieved by applying a window on the neutron transit time.

  1. Bean counting: fair trade, Delft Reactor Institute shoots neutrons at coffee beans

    NARCIS (Netherlands)

    Schrauwers, A.

    2003-01-01

    Apart from oil, coffee is the worlds most important export product. The large-scale intensive coffee plantations are also one of the worlds biggest users of pesticides. But cautious signs are emerging of return to the traditional, practically pesticide-free, organic growing methods, a trend that

  2. Managing the risks of a large-scale infrastructure project: The case of Spoorzone Delft

    NARCIS (Netherlands)

    Priemus, H.

    2012-01-01

    Risk management in large-scale infrastructure projects is attracting the attention of academics and practitioners alike. After a brief summary of the theoretical background, this paper describes how the risk analysis and risk management shaped up in a current large-scale infrastructure project in

  3. Delft3D-Modelling of Hydrodynamic and Water Quality Processes in San Diego Bight

    Science.gov (United States)

    2010-09-30

    Terrill (SIO Scripps), Dr. Lyle Hibler ( PNL ) and Mark Moline (CalPoly) who are funded separately. LONG-TERM GOALS The long-term goal of this effort is...October 3, 2002. RELATED PROJECTS This work is a cooperation with Dr. Eric Terrill (SIO Scripps), Dr. Lyle Hibler ( PNL ) and Mark Moline (CalPoly) who are funded separately. 5

  4. Delfts blauwe dood: de gevolgen van de cholera in de negentiende eeuw

    NARCIS (Netherlands)

    Ekamper, P.; Buzing, G.

    2013-01-01

    Cholera-epidemieën waren in de 19de eeuw verantwoordelijk voor vele dodelijke slachtoffers. De ziekte maakte bovendien geen onderscheid tussen arm en rijk. Normaal gesproken relatief minder kwetsbare groepen in die tijd, zoals wat oudere kinderen en jongere volwassenen, liepen juist een groter

  5. Innovative collection development for e-books at the TU Delft Library

    NARCIS (Netherlands)

    Brinkman Dzwig, Z.E.

    2013-01-01

    Thanks to new technologies libraries worldwide go digital and are accessible 24/7 from remote locations. The innovation is even more visible in traditional library tasks such as collection development and acquisitions. Those tasks are undergoing rapid transformation as well. One of the reasons is

  6. Filtration Characterization Method as Tool to Assess Membrane Bioreactor Sludge Filterability—The Delft Experience

    NARCIS (Netherlands)

    Lousada-Ferreira, M.; Krzeminski, P.; Geilvoet, S.; Moreau, A.; Gil, J.A.; Evenblij, H.; Van Lier, J.B.; Van der Graaf, J.H.J.M.

    2014-01-01

    Prevention and removal of fouling is often the most energy intensive process in Membrane Bioreactors (MBRs), responsible for 40% to 50% of the total specific energy consumed in submerged MBRs. In the past decade, methods were developed to quantify and qualify fouling, aiming to support optimization

  7. Filtration characterization method as tool to assess membrane bioreactor sludge filterability-the Delft experience

    OpenAIRE

    Maria Lousada-Ferreira; Pawel Krzeminski; Stefan Geilvoet; Adrien Moreau; Jose A. Gil; Herman Evenblij; van Lier, Jules B.; van der Graaf, Jaap H.J.M.

    2014-01-01

    Prevention and removal of fouling is often the most energy intensive process in Membrane Bioreactors (MBRs), responsible for 40% to 50% of the total specific energy consumed in submerged MBRs. In the past decade, methods were developed to quantify and qualify fouling, aiming to support optimization in MBR operation. Therefore, there is a need for an evaluation of the lessons learned and how to proceed. In this article, five different methods for measuring MBR activated sludge filterability an...

  8. Pilot application of Delft3D Flexible Mesh: assisting a field campaign at the sand engine

    NARCIS (Netherlands)

    Luijendijk, Arjen; Braat, L.; Waagmeester, Niek; Scheel, Freek

    2015-01-01

    For the execution of field measurements around the Sand Engine during MegaPEX2014 there was a need for operational model predictions. Local river outflow causes non-uniform velocities and spatially complex current patterns near the Sand Engine. For assessing the optimal timing and location of the in

  9. Design in Industrial Design Engineering: Teaching Design at the School of IDE at the TU Delft.

    Science.gov (United States)

    Schierbeek, Bernd B.

    1988-01-01

    Describes an industrial design course in the Netherlands. Includes a discussion of the school history, integration of various courses, implications from the experience, design process as decision-making, basic design cycle derived from the general problem-solving process, design methodology, creativity, and conclusions. (YP)

  10. Delft3D-FLOW Model of the Yangon Port Area

    NARCIS (Netherlands)

    De Koning, R.J.; Janssen, M.P.J.

    2016-01-01

    Large sedimentation is observed in the Yangon port area in Myanmar and this sediment is blocking the entrance channel into the port. The entrance channel is dredged intensively to keep it navigable but insight into the sedimentation processes is limited. The sedimented area rises above the water lev

  11. Managing the risks of a large-scale infrastructure project: The case of Spoorzone Delft

    NARCIS (Netherlands)

    Priemus, H.

    2012-01-01

    Risk management in large-scale infrastructure projects is attracting the attention of academics and practitioners alike. After a brief summary of the theoretical background, this paper describes how the risk analysis and risk management shaped up in a current large-scale infrastructure project in th

  12. Delft locust devours documents: safe document destruction services brought to the desktop

    NARCIS (Netherlands)

    Hartmann, L.

    2002-01-01

    You can sometimes spot them in the streets in the Netherlands, the white lorries of the Van Gansewinkel waste disposal company. A while ago the company even had a radio commercial. To emphasize the companys reliability, the message was read by Mr Wil Simon, who used to present the Dutch equivalent

  13. Voorlichting voor het verkeer. Syllabus College Verkeerstechniek T.H. Delft.

    NARCIS (Netherlands)

    Schreuder, D.A.

    1980-01-01

    After a general introduction (1) on the human eye, photometry and sources of light, the report deals with the functional aspects of public lighting (2), its design (3), and the cost benefit analysis (4). In chapter 2 the function of traffic provisions in general and those of traffic lighting are

  14. Innovative collection development for e-books at the TU Delft Library

    NARCIS (Netherlands)

    Brinkman Dzwig, Z.E.

    2013-01-01

    Thanks to new technologies libraries worldwide go digital and are accessible 24/7 from remote locations. The innovation is even more visible in traditional library tasks such as collection development and acquisitions. Those tasks are undergoing rapid transformation as well. One of the reasons is t

  15. Duurzame energietechniek : toegepast in de bloembollensector : bijlage rapport : Afstudeerscriptie WErktuigbouwkunde De Haagse Hogeschool afdeling Delft

    NARCIS (Netherlands)

    Meij, van der M.; PPO Bloembollenteelt,

    2011-01-01

    In dit rapport wordt een overzicht gegeven van duurzame energietechnieken die op bloembollenbedrijven toegepast kunnen worden. De volgende technieken zijn onderzocht: zonnepanelen, windenergie, zonnedak, warmtepomp, geothermie, warmtekrachtkoppeling en houtkachel.

  16. Modeling tidal dynamics in a mangrove creek catchment in Delft3D

    NARCIS (Netherlands)

    Horstman, E.M.; Dohmen-Janssen, C.M.; Hulscher, S.J.M.H.; Bonneton, P.; Garlan, T.

    2013-01-01

    Modeling tidal dynamics in mangroves is of great use in studying the effects of changes in e.g. vegetation cover or tidal forcing. Process based models, taking into account vegetation drag and turbulence, have not yet been applied to study tidal dynamics in mangrove forests. We compare three differe

  17. Aging of Asphalt Symposium, September 17th 2014, Delft, the Netherlands

    NARCIS (Netherlands)

    Erkens, S.M.J.G.; Scarpas, A.; Blab, R.; Glaser, R.; Glover, C.; Oeser, M.; Porot, L.; Soenen, H.

    2015-01-01

    Technical specifications for the asphalt concrete properties are developed to be able to specify mixtures that will perform well in pavement applications. Being able to identify and determine properties related to pavement performance in practice is crucial for both road authorities and contractors,

  18. Aging of Asphalt Symposium, September 17th 2014, Delft, the Netherlands

    OpenAIRE

    Erkens, S.M.J.G.; Scarpas, A.; Blab, R.; Glaser, R; Glover, C.; Oeser, M.; Porot, L.; Soenen, H.

    2015-01-01

    Technical specifications for the asphalt concrete properties are developed to be able to specify mixtures that will perform well in pavement applications. Being able to identify and determine properties related to pavement performance in practice is crucial for both road authorities and contractors, since it allows for design and risk management by determining design life times and reliability. However, the properties of Asphalt Concrete (AC) change over its lifetime and since most pavement l...

  19. A suspended-load experiment in a straight flume at Delft Hydraulics

    NARCIS (Netherlands)

    Talmon, A.M.; De Graaff, J.

    1991-01-01

    A suspended sediment transport experiment in a straight flume with a mobile bed is reported. The bed topography is non-horizontal. The flow is steady. Due to an obstruction in the entrance a steady bed oscillation is generated. The bed topography is measured by means of manual bed soundings (by Del

  20. Morphological modelling of the Western Scheldt: validation of DELFT3D

    OpenAIRE

    Kuijper, C.; Steijn, R.C.; D. Roelvink; van der Kaaij, T.; Olijslagers, P.

    2004-01-01

    Morphological changes of the Scheldt estuary are extensively monitored by the Dutch and Belgian authorities. To predict effects of long-term natural changes (sea level rise) and the consequences of various human interventions, such as dredging/dumping/sand mining and deepening of the navigation channel, there is an urgent need for adequate and reliable tools in these matters. The present report describes the results of the calibration and verification of the DEFLT3D morphological model for th...

  1. Imaging and nanofabrication with the helium ion microscope of the Van Leeuwenhoek Laboratory in Delft.

    Science.gov (United States)

    Alkemade, Paul F A; Koster, Emma M; van Veldhoven, Emile; Maas, Diederik J

    2012-01-01

    Although helium ion microscopy (HIM) was introduced only a few years ago, many new application fields are emerging. The connecting factor between these novel applications is the unique interaction of the primary helium ion beam with the sample material at and just below its surface. In particular, the HIM secondary electron signal stems from an area that is extremely well localized around the point of incidence of the primary beam. This makes the HIM well suited for both high-resolution imaging and high-resolution nanofabrication. Another advantage in nanofabrication is the low ion backscattering fraction, which leads to a weak proximity effect. The subnanometer probe size and the unique beam-materials interactions have opened new areas of research. This review presents a selection of studies conducted on a single instrument. The selection encompasses applications ranging from imaging to nanofabrication and from fundamental academic research to applied industrial developments.

  2. Opening van vier nieuwe laboratoria van de Afdeling der Werktuigbouwkunde van de Technische Hogeschool Delft

    NARCIS (Netherlands)

    Boon, E.F.; Boot, J.; Vahl, L.; Van Eldik Thieme, H.C.A.; Blok, H.

    1959-01-01

    Samenvatting van de toespraak door Prof.ir, R. van Hasselt bij de officiele opening van vier nieuwe laboratoriavan de Afdeling der Werktuigbouwkunde Boon, E.F. en Boot, J.: het Laboratorium voor Chemische Werktuigen Vahl, L.: het Laboratorium voor Koudetechniek Van Eldik Thieme, H.C.A.: het

  3. Bean counting: fair trade, Delft Reactor Institute shoots neutrons at coffee beans

    NARCIS (Netherlands)

    Schrauwers, A.

    2003-01-01

    Apart from oil, coffee is the worlds most important export product. The large-scale intensive coffee plantations are also one of the worlds biggest users of pesticides. But cautious signs are emerging of return to the traditional, practically pesticide-free, organic growing methods, a trend that sta

  4. Delfts blauwe dood: de gevolgen van de cholera in de negentiende eeuw

    NARCIS (Netherlands)

    Ekamper, P.; Buzing, G.

    2013-01-01

    Cholera-epidemieën waren in de 19de eeuw verantwoordelijk voor vele dodelijke slachtoffers. De ziekte maakte bovendien geen onderscheid tussen arm en rijk. Normaal gesproken relatief minder kwetsbare groepen in die tijd, zoals wat oudere kinderen en jongere volwassenen, liepen juist een groter risic

  5. Delft locust devours documents: safe document destruction services brought to the desktop

    NARCIS (Netherlands)

    Hartmann, L.

    2002-01-01

    You can sometimes spot them in the streets in the Netherlands, the white lorries of the Van Gansewinkel waste disposal company. A while ago the company even had a radio commercial. To emphasize the companys reliability, the message was read by Mr Wil Simon, who used to present the Dutch equivalent o

  6. Delfts blauwe dood: de gevolgen van de cholera in de negentiende eeuw

    NARCIS (Netherlands)

    Ekamper, P.; Buzing, G.

    2013-01-01

    Cholera-epidemieën waren in de 19de eeuw verantwoordelijk voor vele dodelijke slachtoffers. De ziekte maakte bovendien geen onderscheid tussen arm en rijk. Normaal gesproken relatief minder kwetsbare groepen in die tijd, zoals wat oudere kinderen en jongere volwassenen, liepen juist een groter risic

  7. Reappraisal of the diagnostic and prognostic value of morning stiffness in arthralgia and early arthritis: Results from the Groningen EARC, Leiden EARC, ESPOIR, Leiden EAC and REACH

    NARCIS (Netherlands)

    J.A.B. van Nies (Jessica A.B.); C. Alves (Celina); A.L.S. Radix-Bloemen (Audrey L.S.); C. Gaujoux-Viala (Cécile); T.W.J. Huizinga (Tom); J.M.W. Hazes (Mieke); E. Brouwer (Eric); B. Fautrel (Bruno); Mil, A.H.M.H.-V. (Annette H.M. van der Helm-van)

    2015-01-01

    textabstractIntroduction: Morning stiffness is assessed daily in the diagnostic process of arthralgia and arthritis, but large-scale studies on the discriminative ability are absent. This study explored the diagnostic value of morning stiffness in 5,202 arthralgia and arthritis patients and the prog

  8. Reappraisal of the diagnostic and prognostic value of morning stiffness in arthralgia and early arthritis : results from the Groningen EARC, Leiden EARC, ESPOIR, Leiden EAC and REACH

    NARCIS (Netherlands)

    van Nies, Jessica A. B.; Alves, Celina; Radix-Bloemen, Audrey L. S.; Gaujoux-Viala, Cecile; Huizinga, Tom W. J.; Hazes, Johanna M. W.; Brouwer, Elisabeth; Fautrel, Bruno; van der Helm-van Mil, Annette H. M.

    2015-01-01

    Introduction: Morning stiffness is assessed daily in the diagnostic process of arthralgia and arthritis, but large-scale studies on the discriminative ability are absent. This study explored the diagnostic value of morning stiffness in 5,202 arthralgia and arthritis patients and the prognostic value

  9. Rewards and road user behaviour : an investigation of the effects of reward programs on safety belt use. Proefschrift Rijksuniversiteit Leiden RUL, Leiden.

    NARCIS (Netherlands)

    Hagenzieker, M.P.

    1999-01-01

    It has been traditional practice in the traffic system for desired behaviour to be stimulated by regulations and laws, resulting in the punishment of offences. Until recently, modifying road user behaviour by rewards has not been given much attention in the practice of road safety. The aim of this m

  10. The Lightweight Delft Cylinder Hand, the First Multi-Articulating Hand That Meets the Basic User Requirements

    NARCIS (Netherlands)

    Smit, G.; Plettenburg, D.H.; Van der Helm, F.C.T.

    2014-01-01

    Rejection rates of upper limb prostheses are high (23-45%). Amputees indicate that the highest design priority should be reduction of the mass of the prosthetic device. Despite all efforts, the mass of the new prosthetic hands is 35-73% higher than that of older hands. Furthermore current hands are

  11. First Quantification of Calcium Intake from Calcium-Dense Dairy Products in Dutch Fracture Patients (The Delft Cohort Study

    Directory of Open Access Journals (Sweden)

    Peter van den Berg

    2014-06-01

    Full Text Available Recommendations for daily calcium intake from dairy products are variable and based on local consensus. To investigate whether patients with a recent fracture complied with these recommendations, we quantified the daily dairy calcium intake including milk, milk drinks, pudding, yoghurt, and cheese in a Dutch cohort of fracture patients and compared outcomes with recent data of a healthy U.S. cohort (80% Caucasians. An observational study analyzed dairy calcium intakes of 1526 female and 372 male Dutch fracture patients older than 50. On average, participants reported three dairy servings per day, independently of age, gender or population density. Median calcium intake from dairy was 790 mg/day in females and males. Based on dairy products alone, 11.3% of women and 14.2% of men complied with Dutch recommendations for calcium intake (adults ≤ 70 years: 1100 mg/day and >70 years: 1200 mg/day. After including 450 mg calcium from basic nutrition, compliance raised to 60.5% and 59.1%, respectively, compared to 53.2% in the U.S. cohort. Daily dairy calcium intake is not associated with femoral neck bone mineral density (BMD T-scores or WHO Fracture Assessment Tool (FRAX risk scores for major fracture or hip fracture. However, when sub analyzing the male cohort, these associations were weakly negative. The prevalence of maternal hip fracture was a factor for current fracture risks, both in women and men. While daily dairy calcium intake of Dutch fracture patients was well below the recommended dietary intake, it was comparable to intakes in a healthy U.S. cohort. This questions recommendations for adding more additional dairy products to preserve adult skeletal health, particularly when sufficient additional calcium is derived from adequate non-dairy nutrition.

  12. La experiencia del espacio academico flexible: BK-City, Universidad Tecnica de Delft, laboratorio spacial de una facultad de arquitectura

    National Research Council Canada - National Science Library

    Penaloza Caicedo, Andreia; Curvelo Magdaniel, Flavia

    2012-01-01

    ...? La anterior pregunta se enmarca dentro del concepto de flexibilidad como herramienta de diseno que permite explorar y proponer modelos espaciales adaptables a las condiciones dinamicas del proceso...

  13. First quantification of calcium intake from calcium-dense dairy products in Dutch fracture patients (the Delft cohort study).

    Science.gov (United States)

    van den Berg, Peter; van Haard, Paul M M; van den Bergh, Joop P W; Niesten, Dieu Donné; van der Elst, Maarten; Schweitzer, Dave H

    2014-06-23

    Recommendations for daily calcium intake from dairy products are variable and based on local consensus. To investigate whether patients with a recent fracture complied with these recommendations, we quantified the daily dairy calcium intake including milk, milk drinks, pudding, yoghurt, and cheese in a Dutch cohort of fracture patients and compared outcomes with recent data of a healthy U.S. cohort (80% Caucasians). An observational study analyzed dairy calcium intakes of 1526 female and 372 male Dutch fracture patients older than 50. On average, participants reported three dairy servings per day, independently of age, gender or population density. Median calcium intake from dairy was 790 mg/day in females and males. Based on dairy products alone, 11.3% of women and 14.2% of men complied with Dutch recommendations for calcium intake (adults ≤ 70 years: 1100 mg/day and >70 years: 1200 mg/day). After including 450 mg calcium from basic nutrition, compliance raised to 60.5% and 59.1%, respectively, compared to 53.2% in the U.S. cohort. Daily dairy calcium intake is not associated with femoral neck bone mineral density (BMD) T-scores or WHO Fracture Assessment Tool (FRAX) risk scores for major fracture or hip fracture. However, when sub analyzing the male cohort, these associations were weakly negative. The prevalence of maternal hip fracture was a factor for current fracture risks, both in women and men. While daily dairy calcium intake of Dutch fracture patients was well below the recommended dietary intake, it was comparable to intakes in a healthy U.S. cohort. This questions recommendations for adding more additional dairy products to preserve adult skeletal health, particularly when sufficient additional calcium is derived from adequate non-dairy nutrition.

  14. Data consistency checks for building a 3D model: A case study of Technical University, Delft Campus, The Netherlands

    NARCIS (Netherlands)

    Ghawana, T.; Zlatanova, S.

    2010-01-01

    GIS is changing rapidly its face with the advancement of computing technologies. From merely a 2D representation of real world features, it is moving to present more and more applications in 3D formats. Today 3D GIS is an essential way of handling the spatial data in urban planning. In cities where

  15. Data consistency checks for building a 3D model: A case study of Technical University, Delft Campus, The Netherlands

    NARCIS (Netherlands)

    Ghawana, T.; Zlatanova, S.

    2010-01-01

    GIS is changing rapidly its face with the advancement of computing technologies. From merely a 2D representation of real world features, it is moving to present more and more applications in 3D formats. Today 3D GIS is an essential way of handling the spatial data in urban planning. In cities where

  16. The Why Factory Tribune, Delft, The Netherlands%荷兰代尔夫特Why Factory讲堂

    Institute of Scientific and Technical Information of China (English)

    MVRDV建筑师事务所

    2011-01-01

    @@ Why Factory是由Winy Maas于2008年创立并领导的一个全球城市问题智囊团和研究机构.就在Why Factory刚移入代尔夫特理工大学的建筑系馆的几周后,该馆于2008年5月12日被大火烧毁.

  17. Red light running by cyclists : which factors influence the red light negating cyclist? Thesis Delft University of Technology.

    NARCIS (Netherlands)

    Meel, E.M. van der

    2013-01-01

    In an ideal world there would be no crashes in traffic. Unfortunately the real world is far from ideal and over 20,000 people get injured in a crash in the Netherlands each year. Around 46,7% of these registered crashes, take place at intersections. To decrease the chance and number of conflicts, an

  18. Teaching nanoscience across scientific and geographical borders A European Master programme in nanoscience and nanotechnology

    Science.gov (United States)

    Chesneau, A.; Groeseneken, G.; Heremans, P.; Rep, D.; Rudquist, P.; Schwille, P.; Sluijter, B.; Wendin, G.

    2008-03-01

    Within the Erasmus Mundus Master (EMM) Programme, five European Universities (KU Leuven, Belgium, Chalmers University of Technology, Sweden, Delft University of Technology and Leiden University, the Netherlands, and the University of Dresden, Germany) have joined forces to offer a unique master programme in Nanoscience and Nanotechnology, 'EMM-nano', at the cutting edge of state-of-the-art research. The students design and build their individual area of specialisation within nanophysics, nanotechnology, biophysics, biotechnology through their choice of trajectory between the partners. We discuss some of the challenges related to the crossdisciplinary nature of the field, educational activities in cleanrooms, and issues related to the integration of teaching programmes across the borders within Europe.

  19. High blood pressure at old age : The Leiden 85 plus study

    NARCIS (Netherlands)

    Bemmel, Thomas van

    2010-01-01

    The last decades have shown an increasing interest in treatment of high blood pressure. Copious amounts of data have been published on the mortality and morbidity risks of high blood pressure. Overall these data have resulted in an increasing awareness of the deleterious effects of only modest eleva

  20. Species of Ganoderma and related genera mainly of the Bogor and Leiden Herbaria

    NARCIS (Netherlands)

    Steyaert, R.L.

    1972-01-01

    This contribution to the knowledge of the Aphyllophorales is dedicated to Dr. M. A. Donk as a tribute to his life-long endeavour to put the systematics and the taxonomy of this challenging order on a sound basis. A collection of South Asian specimens of Ganoderma and various other collections have b

  1. Onderzoeken moeten leiden tot praktische tips ter voorkoming: Komkommerbontvirus: veel vragen, antwoorden niet eenvoudig

    NARCIS (Netherlands)

    Stijger, I.

    2011-01-01

    Komkommerbontvirus (CGMMV) is een groot probleem op komkommerbedrijven. Het virus is erg hardnekkig en de afgelopen jaren hebben steeds meer bedrijven er mee te maken gehad. Komkommertelers doen er zo veel mogelijk aan om het virus uit de kas te houden en verspreiding te voorkomen. Dat blijkt niet e

  2. Actiniaria from Ria de Arosa, Galicia, northwestern Spain, in the Netherlands Centre for Biodiversity Naturalis, Leiden

    NARCIS (Netherlands)

    Hartog, den J.C.; Ates, R.M.L.

    2011-01-01

    Twentysix species of sea anemones (Actiniaria) collected in Galicia, northwestern Spain are present in the collection of the Netherlands Centre for Biodiversity Naturalis. They are listed and discussed. The cnidom of seventeen species is surveyed. Diadumene cincta is not a recent immigrant in wester

  3. Species of Ganoderma and related genera mainly of the Bogor and Leiden Herbaria

    NARCIS (Netherlands)

    Steyaert, R.L.

    1972-01-01

    This contribution to the knowledge of the Aphyllophorales is dedicated to Dr. M. A. Donk as a tribute to his life-long endeavour to put the systematics and the taxonomy of this challenging order on a sound basis. A collection of South Asian specimens of Ganoderma and various other collections have

  4. Identification of prognostic and diagnostic biomarkers of glucose intolerance in ApoE3Leiden mice

    NARCIS (Netherlands)

    Wopereis, S.; Radonjic, M.; Rubingh, C.; Erk, M. van; Smilde, A.; Duyvenvoorde, W. van; Cnubben, N.; Kooistra, T.; Ommen, B. van; Kleemann, R.

    2012-01-01

    The prevalence of diabetes mellitus Type 2 could be significantly reduced by early identification of subjects at risk, allowing for better prevention and earlier treatment. Glucose intolerance (GI) is a hallmark of the prediabetic stage. This study aims at identifying 1) prognostic biomarkers predic

  5. A systematic catalogue of the Coleopterous family Helotidae in the Leiden Museum

    NARCIS (Netherlands)

    Ritsema, C.

    1915-01-01

    Gen. HELOTA Mc L.1) Mc Leay, Annulosa Javanica, 1825, p. 42; ed. Lequien, 1833, p. 151. INDOMALAYAN SPECIES. (Nos. 1—69). 1. Vigorsi Mc L. Annul. Javan. 1825, p. 43, tab. 1, fig. 9; ed. Lequien, 1833, p. 152, tab. 5, fig. 4. a. ♀. Java or.: Mt. Ardjoeno (W. E. J. Hekmeyer). b. ♀. Java (A. B. v.

  6. Polychaeten aus den Zoologischen Museen von Leiden und Amsterdam. - IV. (Schluss)

    NARCIS (Netherlands)

    Augener, H.

    1934-01-01

    Fam. ARICIIDAE. Aricia foetida Clap. Fundort: 73° N. 25° 0. 220 fm. 19.VII. 1878. -W. B. 1878/79. — M. L. Von diesem Fundort liegen zwei hinten mehr oder weniger unvollständige Exemplare vor, von denen das kürzere mit noch 95 Segmenten ca. 33 mm lang ist. Ich bemerke über dieses Tier folgendes. Die

  7. Integraal verkeerstoezicht in de subregio Leiden : effecten op het gebruik van autogordels.

    NARCIS (Netherlands)

    Mathijssen, M.P.M.

    1993-01-01

    In 1990, the national and local police carried out an experimental integral traffic enforcement policy in the subregion of Leyden (situated in the western part of the Netherlands). The purpose of the experiment (See also IRRD 855463 and 855464) was to combine the enforcement on different important

  8. Aesthesis in anatomy. Materiality and elegance in the Eighteenth-Century Leiden Anatomical Collections

    NARCIS (Netherlands)

    Hendriksen, Marieke Maria Anna

    2012-01-01

    A life-like human arm decorated with lace sleeves, holding an eyelid on a string, fragments of skin swivelling in a phial with a twig, a shiny silvery liver, a dog with a cleft palate, a human ear with a tiny pox mark, foetuses decorated with colourful beads. At first sight, it seems an odd collecti

  9. High blood pressure at old age : The Leiden 85 plus study

    NARCIS (Netherlands)

    Bemmel, Thomas van

    2010-01-01

    The last decades have shown an increasing interest in treatment of high blood pressure. Copious amounts of data have been published on the mortality and morbidity risks of high blood pressure. Overall these data have resulted in an increasing awareness of the deleterious effects of only modest

  10. Mesenteric and portal vein thrombosis associated with hyperhomocysteinemia and heterozygosity for factor V Leiden mutation

    Institute of Scientific and Technical Information of China (English)

    Giuseppe Famularo; Giovanni Minisola; Giulio Cesare Nicotra; Claudio De Simone

    2005-01-01

    @@ TO THE EDITOR A 79-year-old man was hospitalized because of worsening upper abdominal pain which started two days before admission and was continuously present. His personal and family historywas uneventful, he did not smoke and denied toxic habits or using any medications, including over the-counter medications, herbal remedies or any vitamin supplements.

  11. From Innovation Community to Community Innovation User-initiated Innovation in Wireless Leiden

    NARCIS (Netherlands)

    van Oost, Elizabeth C.J.; Verhaegh, Stefan; Oudshoorn, Nelly E.J.

    2009-01-01

    The role of users in innovation processes has gained increasing attention in innovation studies, technology studies, and media studies. Scholars have identified users and use practices as a source of innovation. So far, however, little insight has been generated in innovation processes in which

  12. Reference values for anxiety questionnaires: the Leiden Routine Outcome Monitoring Study.

    Science.gov (United States)

    Schulte-van Maaren, Yvonne W M; Giltay, Erik J; van Hemert, Albert M; Zitman, Frans G; de Waal, Margot W M; Carlier, Ingrid V E

    2013-09-25

    The monitoring of patients with an anxiety disorder can benefit from Routine Outcome Monitoring (ROM). As anxiety disorders differ in phenomenology, several anxiety questionnaires are included in ROM: Brief Scale for Anxiety (BSA), PADUA Inventory Revised (PI-R), Panic Appraisal Inventory (PAI), Penn State Worry Questionnaire (PSWQ), Worry Domains Questionnaire (WDQ), Social Interaction, Anxiety Scale (SIAS), Social Phobia Scale (SPS), and the Impact of Event Scale-Revised (IES-R). We aimed to generate reference values for both 'healthy' and 'clinically anxious' populations for these anxiety questionnaires. We included 1295 subjects from the general population (ROM reference-group) and 5066 psychiatric outpatients diagnosed with a specific anxiety disorder (ROM patient-group). The MINI was used as diagnostic device in both the ROM reference group and the ROM patient group. To define limits for one-sided reference intervals (95th percentile; P95) the outermost 5% of observations were used. Receiver Operating Characteristics (ROC) analyses were used to yield alternative cut-off values for the anxiety questionnaires. For the ROM reference-group the mean age was 40.3 years (SD=12.6), and for the ROM patient-group it was 36.5 years (SD=11.9). Females constituted 62.8% of the reference-group and 64.4% of the patient-group. P95 ROM reference group cut-off values for reference versus clinically anxious populations were 11 for the BSA, 43 for the PI-R, 37 for the PAI Anticipated Panic, 47 for the PAI Perceived Consequences, 65 for the PAI Perceived Self-efficacy, 66 for the PSWQ, 74 for the WDQ, 32 for the SIAS, 19 for the SPS, and 36 for IES-R. ROC analyses yielded slightly lower reference values. The discriminative power of all eight anxiety questionnaires was very high. Substantial non-response and limited generalizability. For eight anxiety questionnaires a comprehensive set of reference values was provided. Reference values were generally higher in women than in men, implying the use of gender-specific cut-off values. Each instrument can be offered to every patient with MAS disorders to make responsible decisions about continuing, changing or terminating therapy. © 2013 Elsevier B.V. All rights reserved.

  13. Lymphocyte count and mortality risk in older persons. The Leiden 85-Plus Study

    NARCIS (Netherlands)

    Izaks, GJ; Remarque, EJ; Becker, SV; Westendorp, RGJ

    2003-01-01

    OBJECTIVES: To investigate whether a low peripheral blood lymphocyte count is associated with increased mortality risk in older persons and to determine whether this association could be ascribed to ill health. DESIGN: A cohort study with a total follow-up period of 1,602 person years. SETTING: Leid

  14. Einige Ergebnisse der Sichtung der Gattungen Melitaea und Argynnis im Rijksmuseum van Natuurlijke Historie, Leiden

    NARCIS (Netherlands)

    Eisner, C.

    1942-01-01

    Melitaea cinxia L. subsp. balcanensis nov. subsp. ♂ ähnelt dem von subsp. australis Vrty., glänzend braun mit prägnanter Schwarzzeichnung. ♀ fahlgelb, oft grün überstäubt, meist mit zurücktretender Schwarzzeichnung; zwei Exemplare indessen dunkel, f. obscurior Stgr. Die Unterseite wie die von subsp.

  15. Michael Reid Trice, Encountering Cruelty: The Fracture of the Human Heart (Leiden and Boston: Brill, 2011)

    OpenAIRE

    Wendy Hamblet

    2012-01-01

    As many violence scholars, I have not found myself to be greatly interested in cruelty, for the simple reason that instances of cruelty are relatively rare, if we accept its usual definition as “willful indifference to suffering, or taking pleasure in inflicting such suffering upon sentient beings.” Most people do not actively wish harm to others, and for this reason, the harms that keep our societies running smoothly and in ordered fashion must be kept in concealment, far from our direct vis...

  16. Michael Reid Trice, Encountering Cruelty: The Fracture of the Human Heart (Leiden and Boston: Brill, 2011

    Directory of Open Access Journals (Sweden)

    Wendy Hamblet

    2012-03-01

    Full Text Available As many violence scholars, I have not found myself to be greatly interested in cruelty, for the simple reason that instances of cruelty are relatively rare, if we accept its usual definition as “willful indifference to suffering, or taking pleasure in inflicting such suffering upon sentient beings.” Most people do not actively wish harm to others, and for this reason, the harms that keep our societies running smoothly and in ordered fashion must be kept in concealment, far from our direct vision, if we are to be asked to tolerate them—cruel punishments, executions, exclusions of minorities from the full benefits of the society, etc. However, Michael Trice’s new book, Encountering Cruelty, renews a fresh interest in this dark topic by redefining cruelty as an ordinary, common occurrence, infecting the everyday in ever more subtle forms. Trice reveals how a too narrow definition of cruelty may grant us the comfort of believing its occurrence to be minimal, but it robs us of the opportunity to track its traces writ large across the landscape of the human world. We cannot hope to understand, and ultimately heal, the rebounding effects of suffering across the broader social terrain, unless we are willing to admit the cruelty that underlies modern institutional life.

  17. Endocrine and metabolic features of familial longevity : the Leiden Longevity Study

    NARCIS (Netherlands)

    Rozing, Maarten Pieter

    2011-01-01

    This thesis discusses the metabolic and endocrine characteristics of long-lived Dutch families. We found that familial longevity is marked by low thyroid function and preserved insulin senitivity. The second part of this thesis adresses the Gompertz law of mortality as an estimate of the rate of sen

  18. Levels of 25-hydroxyvitamin D in familial longevity: the Leiden Longevity Study

    NARCIS (Netherlands)

    Noordam, R.; Craen, A.J.M.; Pedram, P.; Maier, A.B.; Mooijaart, S.P.; Pelt, van J.; Feskens, E.J.M.; Streppel, M.T.; Slagboom, P.E.; Westendorp, R.G.; Beekman, M.; Heemst, van D.

    2012-01-01

    Background: Low levels of 25(OH) vitamin D are associated with various age-related diseases and mortality, but causality has not been determined. We investigated vitamin D levels in the offspring of nonagenarians who had at least one nonagenarian sibling; these offspring have a lower prevalence of a

  19. A systematic catalogue of the Coleopterous family Helotidae in the Leiden Museum

    NARCIS (Netherlands)

    Ritsema, C.

    1915-01-01

    Gen. HELOTA Mc L.1) Mc Leay, Annulosa Javanica, 1825, p. 42; ed. Lequien, 1833, p. 151. INDOMALAYAN SPECIES. (Nos. 1—69). 1. Vigorsi Mc L. Annul. Javan. 1825, p. 43, tab. 1, fig. 9; ed. Lequien, 1833, p. 152, tab. 5, fig. 4. a. ♀. Java or.: Mt. Ardjoeno (W. E. J. Hekmeyer). b. ♀. Java (A. B. v. Mede

  20. Science and Taste: Painting, Passions, and the New Philosophy in Seventeenth-Century Leiden

    National Research Council Canada - National Science Library

    Pamela H. Smith

    1999-01-01

    ... throughout Europe in the late seventeenth and eighteenth centuries. Sylvius's house design and large painting collection also exemplified the consumption and taste of the northern Netherlands in the seventeenth century...

  1. Buprestides du Musée de Leiden (2ème note)

    NARCIS (Netherlands)

    Thery, A.

    1937-01-01

    Cyphogastra Kampeni nov. spec. ♀; Long.: 29 mm; larg.: 9 mm. — Allongé, très atténué postérieurement, ayant sa plus grande largeur vers le tiers postérieur des élytres; entièrement d'un beau vert émeraude à aspect de laque, en dessus, d'un vert légèrement bleuâtre et très brillant, en dessous. Le de

  2. Modulation of HDL metabolism : studies in APOE*3-Leiden.CETP mice

    NARCIS (Netherlands)

    Haan, Willemke de

    2009-01-01

    Cardiovascular disease (CVD) is a major cause of mortality and morbidity in the Western world. CVD is mainly caused by atherosclerosis, for which dyslipidemia, characterized by high a plasma level of (very) low density lipoprotein ((V)LDL) and a low plasma level of high density lipoprotein (HDL), is

  3. Some unusual gemstones in the collection of the Rijksmuseum van Geologie en Mineralogie of Leiden

    NARCIS (Netherlands)

    Zwaan, P.C.

    1955-01-01

    Our Museum can take pride in a very valuable gem collection for, besides the numerous specimens which were gathered by King William I of the Netherlands a great many of the more unusual stones are to be found in it. Especially since World War II the Museum has acquired many interesting and important

  4. Printed and Mimeographed Issues Obtainable from Flora Malesiana Foundation, Rijksherbarium, Leiden

    NARCIS (Netherlands)

    NN,

    1967-01-01

    Index to the Enumeration of the Orchidaceae of Sumatra, by J.J. Smith. At the Rijksherbarium there was a handwritten copy compiled by the late Dr. J.J. Smith of his important enumeration of Sumatran Orchids, published in Fedde, Repertorium 32 (1933) 130-386, which was obviously for reasons of econom

  5. A life of learning in Leiden. The mathematician Frans van Schooten (1615-1660)

    NARCIS (Netherlands)

    Dopper, J.G.

    2014-01-01

    Frans van Schooten jr. (1615-1660) was one of the most influential Dutch mathematicians of the seventeenth century. He is best known for his two Latin editions (1649, 1659-61) of the Géométrie (1637) of Descartes, which originally appeared in French as an appendix to the Discours de la Methode. This

  6. Construction, jobs and affordable energy in the north of the Netherlands; Bouwen, Banen en Betaalbare energie in Noord-Nederland

    Energy Technology Data Exchange (ETDEWEB)

    Blom, M.; Schepers, B. [CE Delft, Delft (Netherlands); Tieben, B. [SEO Economic Research, Amsterdam (Netherlands)

    2013-03-15

    Additional investments to boost the energy efficiency of the housing stock in the north of the Netherlands can create a substantial number of new jobs in construction and supply industries. That is the conclusion of a pilot study carried out by CE Delft and SEO Economic Research. Depending on the energy efficiency target adopted, an annual investment of around euro 400 million would lead to some 4,500 new jobs (in the first year). This would mean over 2,000 unemployed construction workers being able to return to work. To realise these investments requires a 'quantum leap' in funding. For an efficiency improvement scenario of 2% per annum this would mean a quadrupling of present investment plans in this part of the country [Dutch] Extra investeringen in energiebesparing van bestaande woningen kunnen voor Noord-Nederland een fors aantal banen in de bouw en de toeleverende industrie op leveren. Dat is de conclusie uit een verkenning die door CE Delft en SEO Economisch Onderzoek in opdracht van de Natuur en Milieufederatie Drenthe is uitgevoerd. Afhankelijk van de besparingsdoelstelling leiden investeringen van zo'n 400 miljoen per jaar tot zo'n 4.500 extra banen (eerste jaar). Daarmee kunnen ruim 2.000 werkloze bouwvakkers aan het werk worden geholpen. Om deze investeringen te kunnen realiseren, is een forse schaalsprong nodig in het financieringsaanbod. In het scenario van 2%+ per jaar komt dit neer op een verviervoudiging van de huidige investeringsambitie in Noord-Nederland.

  7. Stemcell Information: SKIP000532 [SKIP Stemcell Database[Archive

    Lifescience Database Archive (English)

    Full Text Available yology, Leiden University Medical Center, Leiden, and Interuniversity Cardiology Institute of the Netherland...ty Medical Center, Leiden, and Interuniversity Cardiology Institute of the Netherlands, Utrecht, the Netherl...ands Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, and Interuniversity Car...University Medical Center, Leiden, and Interuniversity Cardiology Institute of th... Embryology, Leiden University Medical Center, Leiden, and Interuniversity Cardiology Institute of the Nethe

  8. Assessment of the relationship between observed crashes and simulated conflicts at intersections. Master of Science Thesis Delft University of Technology, Department of Civil Engineering.

    NARCIS (Netherlands)

    Marchesini, P.A.

    2009-01-01

    The goal of this M.Sc. thesis project is to investigate a traffic safety indicator calculated on the basis of micro-simulation. This safety indicator, TTC (time-to-collision), is based on the concept of a conflict situation; i.e. two vehicles approaching each other in such a way that if they do not

  9. Heavy metal pollution and sediment transport in the rhinemeuse estuary, using a 2D model Delft3D: Water quality and calamities. Case study Biesbosch

    NARCIS (Netherlands)

    Jose Alonso, J.J.

    2010-01-01

    Different measures will be executed in the framework PKB “Room for the River” in order to lower the water levels in the river area during normative high discharges. The plan Ontpoldering Noordwaard is one of them and has as aim to inundate parts of the Noordwaard during high discharges. Water coming

  10. Heavy metal pollution and sediment transport in the rhinemeuse estuary, using a 2D model Delft3D: Water quality and calamities. Case study Biesbosch

    OpenAIRE

    Jose Alonso, J.J.

    2010-01-01

    Different measures will be executed in the framework PKB “Room for the River” in order to lower the water levels in the river area during normative high discharges. The plan Ontpoldering Noordwaard is one of them and has as aim to inundate parts of the Noordwaard during high discharges. Water coming from the Nieuwe Merwede as result of high water levels will flow into the Noordwaard and leave the area through the south part. As consequence the creeks in the Brabantse Biesbosch will process mo...

  11. DASMAT-Delft University Aircraft Simulation Model and Analysis Tool: A Matlab/Simulink Environment for Flight Dynamics and Control Analysis

    NARCIS (Netherlands)

    Van der Linden, C.A.A.M.

    1998-01-01

    Computer Assisted Design (CAD) environments have become important devices for the design and evaluation of flight control systems. For general use, different aircraft and operational conditions should be easily implemented in such a CAD environment and it should be equipped with a set of simulation

  12. DASMAT-Delft University Aircraft Simulation Model and Analysis Tool: A Matlab/Simulink Environment for Flight Dynamics and Control Analysis

    NARCIS (Netherlands)

    Van der Linden, C.A.A.M.

    1998-01-01

    Computer Assisted Design (CAD) environments have become important devices for the design and evaluation of flight control systems. For general use, different aircraft and operational conditions should be easily implemented in such a CAD environment and it should be equipped with a set of simulation

  13. Post-war public housing in trouble: Papers presented at the Congress 'Post-war Public Housing in trouble', Delft, the Netherlands, October 4-5, 1984

    NARCIS (Netherlands)

    Prak, N.L.; Priemus, H.

    1985-01-01

    In a number of countries the social housing sector was given serious food for thought when some relatively new housing estates had to be demolished: Pruitt Igoe (St. Louis), Quarry Hills (Leeds), la Démocratie (Lyon), Linnaeusstraat (Leeuwarden, NL). The management of many other estates proves to be

  14. Assessment of the relationship between observed crashes and simulated conflicts at intersections. Master of Science Thesis Delft University of Technology, Department of Civil Engineering.

    NARCIS (Netherlands)

    Marchesini, P.A.

    2009-01-01

    The goal of this M.Sc. thesis project is to investigate a traffic safety indicator calculated on the basis of micro-simulation. This safety indicator, TTC (time-to-collision), is based on the concept of a conflict situation; i.e. two vehicles approaching each other in such a way that if they do not

  15. Proceedings of the International Symposium Concepts 2020. Road Transport, Vehicles and the Motor Industry into the Next Century, 16 November 1995, Delft, Netherlands

    NARCIS (Netherlands)

    1995-01-01

    In 1995, the TNO Road-Vehicles Research Institute clocked up 25 years as a TNO research institute and we naturally wanted to celebrate this milestone with our many friends and associates at home and abroad.

  16. Post-war public housing in trouble: Papers presented at the Congress 'Post-war Public Housing in trouble', Delft, the Netherlands, October 4-5, 1984

    NARCIS (Netherlands)

    Prak, N.L.; Priemus, H.

    1985-01-01

    In a number of countries the social housing sector was given serious food for thought when some relatively new housing estates had to be demolished: Pruitt Igoe (St. Louis), Quarry Hills (Leeds), la Démocratie (Lyon), Linnaeusstraat (Leeuwarden, NL). The management of many other estates proves to be

  17. Low blood pressure predicts increased mortality in very old age even without heart failure: the Leiden 85-plus Study

    NARCIS (Netherlands)

    Poortvliet, R.K.; Blom, J.W.; Craen, A.J. de; Mooijaart, S.P.; Westendorp, R.G.J.; Assendelft, W.J.J.; Gussekloo, J.; Ruijter, W. de

    2013-01-01

    AIMS: To investigate whether low systolic blood pressure is predictive for increased mortality risk in 90-year-old subjects without heart failure, defined by low levels of NT-proBNP, as well as in 90-year-old subjects with high levels of NT-proBNP. METHODS AND RESULTS: This study was embedded in the

  18. Influence of Factor V Leiden on susceptibility to and outcome from critical illness: a genetic association study

    DEFF Research Database (Denmark)

    Benfield, Thomas; Ejrnaes, Karen; Juul, Klaus

    2010-01-01

    . METHODS: A genetic association study involving four case cohorts comprising two Gram negative sepsis, one invasive pneumococcal disease and one intensive care unit cohort with a total of 1,249 patients. Controls were derived from a population-based cohort study (N = 8,147). DNA from patients and controls...

  19. Felipe II y la conquista de reliquias por los Tercios de Flandes: el ejemplo de Leiden (1570-1574

    Directory of Open Access Journals (Sweden)

    Ferrer García, Félix A.

    2014-06-01

    Full Text Available The patrimonial principles and the exaggerated religious beliefs of Phillip II of Spain, placed in the complex foreign affairs of the 16th century, led to the presence of the castilian troops in many european battlefields. Phillip II had to cope with a raising international hostility, not only due to strategic reasons but also to his clear imperialist ideas. But we can´t avoid some ideological statements because the King considered himself as the true protector of Christianity. The Spanish Tercios, and specially the Duke of Alba, developed military purposes but they were also finders and guardians of the numerous relics from the northern european territories.La concepción patrimonial y el inflamado ímpetu religioso de Felipe II, en el marco de la compleja política exterior del siglo XVI, provocaron la presencia de las tropas castellanas en numerosos campos de batalla europeos. Durante su gobierno el rey contempló la creciente hostilidad internacional, bien por factores estratégicos, bien por un no disimulado afán imperialista, sin olvidar algunos elementos ideológicos, pues el monarca se consideraba defensor de la cristiandad. Los Tercios de Flandes, con el Gran Duque de Alba, ejercitaron su función militar, pero también actuaron como buscadores, acopiadores y custodios de numerosas reliquias depositadas en los territorios europeos septentrionales.

  20. Putative type specimens of Satyrichthys (Scorpaeniformes: Peristediidae) in the Bleeker collection of the Naturalis Biodiversity Center, Leiden, The Netherlands.

    Science.gov (United States)

    Van Oijen, Martien J P; Kawai, Toshio; Loots, Ineke

    2013-01-01

    The presumed type status of the two remaining specimens of Peristedion moluccense Bleeker 1850 in the collection the Naturalis Biodiversity Center was examined by comparing them to descriptions in two Bleeker papers and an unpublished manuscript by Bleeker written for the Atlas Ichthyologique. Latin, French and Dutch parts of these papers were translated into English. The Dutch description of P. laticeps Schlegel 1852 was also translated. In the course of this study it became clear why Bleeker considered P. laticeps, a as a synonym of P. moluccense.

  1. Resultaten van het ringonderzoek betreffende den vogeltrek, ingesteld door 's Rijks Museum van Natuurlijke Historie te Leiden

    NARCIS (Netherlands)

    Oort, van E.D.

    1930-01-01

    De hier volgende berichten van waarneming van geringde vogels werden door mij in 1929 ontvangen. Wederom zijn talrijke interessante gevallen te vermelden, dank zij de medewerking van zoo velen, aan wie ik hier openlijk dank breng. Bij voortduring beveel ik ons ringonderzoek in aller medewerking aan

  2. Resultaten van het ringonderzoek betreffende den vogeltrek, ingesteld door 's Rijks Museum van Natuurlijke Historie te Leiden

    NARCIS (Netherlands)

    Sleijser, A.J.

    1933-01-01

    Het tweede gedeelte van de berichten omtrent waarneming van geringde vogels, die gedurende het jaar 1932 bij 's Rijks Museum van Natuurlijke Historie ingekomen zijn, wordt in de hierna volgende lijst vermeld. Het gemis van Prof. Dr. E. D. van Oort bij het samenstellen van dit verslag is onzegbaar

  3. Outcomes of neuropsychiatric events in systemic lupus erythematosus based on clinical phenotypes; prospective data from the Leiden NP SLE cohort.

    Science.gov (United States)

    Magro-Checa, C; Beaart-van de Voorde, L J J; Middelkoop, H A M; Dane, M L; van der Wee, N J; van Buchem, M A; Huizinga, T W J; Steup-Beekman, G M

    2017-04-01

    Objective The objective of this study was to assess whether clinical and patient's reported outcomes are associated with a different pathophysiological origin of neuropsychiatric events presenting in systemic lupus erythematosus. Methods A total of 232 neuropsychiatric events presenting in 131 systemic lupus erythematosus patients were included. Neuropsychiatric systemic lupus erythematosus diagnosis was established per event by multidisciplinary evaluation. All neuropsychiatric events were divided according to a suspected underlying pathophysiological process into one of the following: non-neuropsychiatric systemic lupus erythematosus related, inflammatory and ischaemic neuropsychiatric systemic lupus erythematosus. The clinical outcome of all neuropsychiatric events was determined by a physician-completed four-point Likert scale. Health-related quality of life was measured with the subscales of the patient-generated Short Form 36 (SF-36) health survey questionnaire. The change between scores at paired visits of all domain scores, mental component summary (SF-36 MCS) and physical component summary (SF-36 PCS) scores were retrospectively calculated and used as patient-reported outcome. The association among these outcomes and the different origin of neuropsychiatric events was obtained using multiple logistic regression analysis. Results The clinical status of 26.8% non-neuropsychiatric systemic lupus erythematosus events, 15.8% ischaemic neuropsychiatric systemic lupus erythematosus and 51.6% inflammatory neuropsychiatric systemic lupus erythematosus improved after re-assessment. Almost all SF-36 domains had a positive change at re-assessment in all groups independently of the origin of neuropsychiatric events. Neuropsychiatric systemic lupus erythematosus ( B = 0.502; p < 0.001) and especially inflammatory neuropsychiatric systemic lupus erythematosus ( B = 0.827; p < 0.001) had better clinical outcome, with change in disease activity being the only important predictor. The change in SF-36 MCS was also independently associated with neuropsychiatric systemic lupus erythematosus ( B = 5.783; p < 0.05) and inflammatory neuropsychiatric systemic lupus erythematosus ( B = 11.133; p < 0.001). Disease duration and change in disease activity were the only predictors in both cases. The change in SF-36 PCS was only negatively associated with age. Conclusion Inflammatory neuropsychiatric systemic lupus erythematosus events have better clinical outcome and meaningful improvement in SF-36 MCS than ischaemic neuropsychiatric systemic lupus erythematosus or non-neuropsychiatric systemic lupus erythematosus.

  4. Value of multidisciplinary reassessment in attribution of neuropsychiatric events to systemic lupus erythematosus: prospective data from the Leiden NPSLE cohort.

    Science.gov (United States)

    Magro-Checa, César; Zirkzee, Els J; Beaart-van de Voorde, Liesbeth J J; Middelkoop, Huub A; van der Wee, Nic J; Huisman, Menno V; Eikenboom, Jeroen; Kruyt, Nyika D; van Buchem, Mark A; Huizinga, Tom W J; Steup-Beekman, Gerda M

    2017-10-01

    To determine the contribution of reassessment in the attribution process of neuropsychiatric (NP) events to SLE or other aetiologies in a large, prospective and multidisciplinary assessed NPSLE cohort and to compare these results with other available attribution models for NP events occurring in SLE. Three hundred and four consecutive SLE patients presenting NP events were evaluated. All subjects underwent standardized multidisciplinary medical, neuropsychological, laboratory and radiological examination on the inclusion and reassessment dates. Diagnosis was always established by multidisciplinary consensus. The final diagnosis after reassessment also took into account disease course and response to treatment. These data were compared with currently available attribution models for NP events in SLE. A total of 463 NP events were established. After reassessment, attribution to SLE was discordant in 64 (13.8%) NP events when compared with the first visit. We show that 14.5% of NP events previously attributed to SLE reclassified as non-NPSLE. In 86.4% of these patients immunosuppressive therapy was started after the first visit. When reassessment and available attribution models were compared, NPSLE cases overlapped considerably. Although specificity was high for all comparisons (0.81-0.95), an important variation in sensitivity (0.39-0.83) and agreement estimates (κ = 0.29-0.68) was observed. The Italian algorithm showed the highest sensitivity and specificity (>0.80) and moderate agreement (0.59-0.64). In clinical practice NP events presenting in SLE are too often attributed to an immune-mediated origin. Multidisciplinary reassessment avoids misclassification in NPSLE. Multidisciplinary reassessment is the reference standard in NP events presenting in SLE and cannot be replaced by available attribution models.

  5. Niacin Reduces Atherosclerosis Development in APOE*3Leiden.CETP Mice Mainly by Reducing NonHDL-Cholesterol

    NARCIS (Netherlands)

    Kühnast, S.; Louwe, M.C.; Heemskerk, M.M.; Pieterman, E.J.; Klinken, J.B. van; Berg, S.A.A. van den; Smit, J.W.A.; Havekes, L.M.; Rensen, P.C.N.; Hoorn, J.W.A. van der; Princen, H.M.G.; Jukema, J.W.

    2013-01-01

    Objective:Niacin potently lowers triglycerides, mildly decreases LDL-cholesterol, and largely increases HDL-cholesterol. Despite evidence for an atheroprotective effect of niacin from previous small clinical studies, the large outcome trials, AIM-HIGH and HPS2-THRIVE did not reveal additional benefi

  6. Niacin Reduces Atherosclerosis Development in APOE*3Leiden.CETP Mice Mainly by Reducing NonHDL-Cholesterol

    NARCIS (Netherlands)

    Kuhnast, S.; Louwe, M.C.; Heemskerk, M.M.; Pieterman, E.J.; Klinken, J.B. van; Berg, S.A. van den; Smit, J.W.A.; Havekes, L.M.; Rensen, P.C.; Hoorn, J.W. van der; Princen, H.M.; Jukema, J.W.

    2013-01-01

    OBJECTIVE: Niacin potently lowers triglycerides, mildly decreases LDL-cholesterol, and largely increases HDL-cholesterol. Despite evidence for an atheroprotective effect of niacin from previous small clinical studies, the large outcome trials, AIM-HIGH and HPS2-THRIVE did not reveal additional benef

  7. Both Transient and Continuous Corticosterone Excess Inhibit Atherosclerotic Plaque Formation in APOE*3-Leiden.CETP Mice

    NARCIS (Netherlands)

    Auvinen, H.E.; Wang, Y.; Princen, H.; Romijn, J.A.; Havekes, L.M.; Smit, J.W.A.; Meijer, O.C.; Biermasz, N.R.; Rensen, P.C.; Pereira, A.M.

    2013-01-01

    INTRODUCTION: The role of glucocorticoids in atherosclerosis development is not clearly established. Human studies show a clear association between glucocorticoid excess and cardiovascular disease, whereas most animal models indicate an inhibitory effect of glucocorticoids on atherosclerosis develop

  8. Both Transient and Continuous Corticosterone Excess Inhibit Atherosclerotic Plaque Formation in APOE*3-Leiden.CETP Mice

    NARCIS (Netherlands)

    Auvinen, H.E.; Wang, Y.; Princen, H.; Romijn, J.A.; Havekes, L.M.; Smit, J.W.A.; Meijer, O.C.; Biermasz, N.R.; Rensen, P.C.N.; Pereira, A.M.

    2013-01-01

    Introduction: The role of glucocorticoids in atherosclerosis development is not clearly established. Human studies show a clear association between glucocorticoid excess and cardiovascular disease, whereas most animal models indicate an inhibitory effect of glucocorticoids on atherosclerosis develop

  9. Low blood pressure predicts increased mortality in very old age even without heart failure: the Leiden 85-plus Study

    NARCIS (Netherlands)

    Poortvliet, R.K.; Blom, J.W.; Craen, A.J. de; Mooijaart, S.P.; Westendorp, R.G.J.; Assendelft, W.J.J.; Gussekloo, J.; Ruijter, W. de

    2013-01-01

    AIMS: To investigate whether low systolic blood pressure is predictive for increased mortality risk in 90-year-old subjects without heart failure, defined by low levels of NT-proBNP, as well as in 90-year-old subjects with high levels of NT-proBNP. METHODS AND RESULTS: This study was embedded in the

  10. Butyrate Reduces HFD-Induced Adipocyte Hypertrophy and Metabolic Risk Factors in Obese LDLr-/-.Leiden Mice

    Directory of Open Access Journals (Sweden)

    Charlotte E. Pelgrim

    2017-07-01

    Full Text Available Adipose tissue (AT has a modulating role in obesity-induced metabolic complications like type 2 diabetes mellitus (T2DM via the production of so-called adipokines such as leptin, adiponectin, and resistin. The adipokines are believed to influence other tissues and to affect insulin resistance, liver function, and to increase the risk of T2DM. In this study, we examined the impact of intervention with the short-chain fatty acid butyrate following a high-fat diet (HFD on AT function and other metabolic risk factors associated with obesity and T2DM in mice during mid- and late life. In both mid- and late adulthood, butyrate reduced HFD-induced adipocyte hypertrophy and elevations in leptin levels, which were associated with body weight, and cholesterol and triglyceride levels. HFD feeding stimulated macrophage accumulation primarily in epididymal AT in both mid- and late life adult mice, which correlated with liver inflammation in late adulthood. In late-adult mice, butyrate diminished increased insulin levels, which were related to adipocyte size and macrophage content in epididymal AT. These results suggest that dietary butyrate supplementation is able to counteract HFD-induced detrimental changes in AT function and metabolic outcomes in late life. These changes underlie the obesity-induced elevated risk of T2DM, and therefore it is suggested that butyrate has potential to attenuate risk factors associated with obesity and T2DM.

  11. Renal transplantation experience in a patient with factor V Leiden homozygous, MTHFR C677T heterozygous, and PAI heterozygous mutation.

    Science.gov (United States)

    Gülhan, Bora; Tavil, Betül; Gümrük, Fatma; Aki, Tuncay F; Topaloglu, Rezan

    2015-08-01

    Vascular complications are important causes of allograft loss in renal transplantation. A two and a half-month-old boy was diagnosed with posterior urethral valve and progressed to end-stage renal disease at eight yr of age. During the HD period, a central venous catheter was replaced three times for repeated thrombosis. The boy was found to be homozygous for FVL and heterozygous for both MTHFR (C677T) and PAI. At the age of 12, renal transplantation was performed from a deceased donor. Postoperative anticoagulation therapy was initiated with continuous intravenous administration of heparin at the dose of 10 IU/kg/h. HD was performed for the first three days. By the fourth day of transplantation, his urine output had increased gradually. Heparin infusion was continued for 18 days during hospitalization at the same dosage. Thereafter, he was discharged with LMWH. On the third month after transplantation, his serum creatinine level was 1.1 mg/dL and eGFR was 75.7 mL/min/1.73 m(2). He has still been using LMWH, and his eGFR was 78.7 mL/min/1.73 m(2) eight months after transplantation. Postoperative low-dose heparin treatment is a safe strategy for managing a patient with multiple thrombotic risk factors. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  12. Plasma and liver lipidomics response to an intervention of rimonabant in ApoE*3Leiden.CETP transgenic mice

    NARCIS (Netherlands)

    Hu, C.; Wei, H.; Hoek, A.M. van den; Wang, M.; Heijden, R. van der; Spijksma, G.; Reijmers, T.H.; Bouwman, J.; Wopereis, S.; Havekes, L.M.; Verheij, E.; Hankemeier, T.; Xu, G.; Greef, J. van der

    2011-01-01

    Background: Lipids are known to play crucial roles in the development of life-style related risk factors such as obesity, dyslipoproteinemia, hypertension and diabetes. The first selective cannabinoid-1 receptor blocker rimonabant, an anorectic anti-obesity drug, was frequently used in conjunction w

  13. Geological collections of the Nationaal Natuurhistorisch Museum (Leiden, The Netherlands): cultural heritage of the geosciences and mining

    NARCIS (Netherlands)

    Winkler Prins, C.F.

    2004-01-01

    The role played by the geological collections of the Nationaal Natuurhistorisch Museum, the National Museum of Natural History, in documenting the developments in the Earth sciences in The Netherlands and abroad is discussed, as well as the influence exercised by the mining industry and former Dutch

  14. Prevalentie van gedragingen van ouders om het huilen van zuigelingen te verminderen die kunnen leiden tot mishandeling.

    NARCIS (Netherlands)

    Reijneveld, S.A.; van der Wal, M.F.; Brugman, E.; Hirasing, R.A.; Verloove-Vanhorick, S.P.

    2004-01-01

    OBJECTIVE: To estimate the prevalence of parental actions to stop infant crying that may threaten infant health, and to determine specific risk groups regarding these actions. DESIGN: Descriptive. METHOD: Before their visit to a well-baby clinic in the Netherlands, parents of 3345 infants aged 1-6 m

  15. Proximity to death is associated with frequency of GP contacts in the oldest old: the Leiden 85-plus study

    NARCIS (Netherlands)

    Blom, J.W.; Lemmens, S.P.; Assendelft, W.J.J.; Eekhof, J.A.H.; Gussekloo, J.

    2012-01-01

    Background: the relationship between proximity to death and the amount of care provided by general practitioners (GPs) is largely unknown. Objective: to examine the influence of the proximity to death on the frequency and length of GP contacts in the oldest old. Study design: this population-based f

  16. Effect of discontinuation of antihypertensive medication on orthostatic hypotension in older persons with mild cognitive impairment: the DANTE Study Leiden.

    Science.gov (United States)

    Moonen, Justine E F; Foster-Dingley, Jessica C; de Ruijter, Wouter; van der Grond, Jeroen; de Craen, Anton J M; van der Mast, Roos C

    2016-03-01

    the relationship between antihypertensive medication and orthostatic hypotension in older persons remains ambiguous, due to conflicting observational evidence and lack of data of clinical trials. to assess the effect of discontinuation of antihypertensive medication on orthostatic hypotension in older persons with mild cognitive impairment. a total of 162 participants with orthostatic hypotension were selected from the Discontinuation of Antihypertensive Treatment in Elderly people (DANTE) Study. This randomised clinical trial included community-dwelling participants aged ≥75 years, with mild cognitive impairment, using antihypertensive medication and without serious cardiovascular disease. Participants were randomised to discontinuation or continuation of antihypertensive treatment (ratio 1:1). Orthostatic hypotension was defined as a drop of at least 20 mmHg in systolic blood pressure and/or 10 mmHg in diastolic blood pressure on standing from a seated position. Outcome was the absence of orthostatic hypotension at 4-month follow-up. Relative risks (RR) were calculated by intention-to-treat and per-protocol analyses. at follow-up, according to intention-to-treat analyses, of the 86 persons assigned to discontinuation of antihypertensive medication, 43 (50%) were free from orthostatic hypotension, compared with 29 (38%) of the 76 persons assigned to continuation of medication [RR 1.31 (95% confidence interval (CI) 0.92-1.87); P = 0.13]. Per-protocol analysis showed that recovery from orthostatic hypotension was significantly higher in persons who completely discontinued all antihypertensive medication (61%) compared with the continuation group (38%) [RR 1.60 (95% CI 1.10-2.31); P = 0.01]. in older persons with mild cognitive impairment and orthostatic hypotension receiving antihypertensive medication, discontinuation of antihypertensive medication may increase the probability of recovery from orthostatic hypotension. © The Author 2016. Published by Oxford University Press on behalf of the British Geriatrics Society. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  17. Effect of Discontinuation of Antihypertensive Treatment in Elderly People on Cognitive Functioning-the DANTE Study Leiden

    DEFF Research Database (Denmark)

    Moonen, Justine E F; Foster-Dingley, Jessica C; de Ruijter, Wouter

    2015-01-01

    ]) and depression (0.14 [-0.20 to 0.48; P = .41]), functional status (-0.72 [-1.52 to 0.09; P = .08]), and quality-of-life score (-0.09 [-0.34 to 0.16; P = .46]). Adverse events were equally distributed. CONCLUSIONS AND RELEVANCE: In older persons with mild cognitive deficits, discontinuation of antihypertensive...... (quality of life). RESULTS: Compared with 176 participants undergoing analysis in the control (continuation) group, 180 in the intervention (discontinuation) group had a greater increase (95% CI) in systolic BP (difference, 7.36 [3.02 to 11.69] mm Hg; P = .001) and diastolic BP (difference, 2.63 [0.34 to 4......IMPORTANCE: Observational studies indicate that lower blood pressure (BP) increases risk for cognitive decline in elderly individuals. Older persons are at risk for impaired cerebral autoregulation; lowering their BP may compromise cerebral blood flow and cognitive function. OBJECTIVE: To assess...

  18. Both Transient and Continuous Corticosterone Excess Inhibit Atherosclerotic Plaque Formation in APOE*3-Leiden.CETP Mice

    NARCIS (Netherlands)

    Auvinen, H.E.; Wang, Y.; Princen, H.; Romijn, J.A.; Havekes, L.M.; Smit, J.W.A.; Meijer, O.C.; Biermasz, N.R.; Rensen, P.C.N.; Pereira, A.M.

    2013-01-01

    Introduction: The role of glucocorticoids in atherosclerosis development is not clearly established. Human studies show a clear association between glucocorticoid excess and cardiovascular disease, whereas most animal models indicate an inhibitory effect of glucocorticoids on atherosclerosis develop

  19. Both Transient and Continuous Corticosterone Excess Inhibit Atherosclerotic Plaque Formation in APOE*3-Leiden.CETP Mice

    NARCIS (Netherlands)

    Auvinen, H.E.; Wang, Y.; Princen, H.; Romijn, J.A.; Havekes, L.M.; Smit, J.W.A.; Meijer, O.C.; Biermasz, N.R.; Rensen, P.C.; Pereira, A.M.

    2013-01-01

    INTRODUCTION: The role of glucocorticoids in atherosclerosis development is not clearly established. Human studies show a clear association between glucocorticoid excess and cardiovascular disease, whereas most animal models indicate an inhibitory effect of glucocorticoids on atherosclerosis develop

  20. Book review of Ida Wendt, EU Competition Law and Liberal Professions: an Uneasy Relationship? Leiden: Brill Academic Publishers, 2012

    NARCIS (Netherlands)

    Vedder, Hans

    2014-01-01

    This review finds this book timely, if only because it highlights the law-making mechanisms that underlie the relevant legislation and jurisprudence. It places the book in the wider context of European integration and suggests further avenues for research.