Introducción. El papel de la biopsia del ganglio centinela realizada después de quimioterapia neoadyuvante es controvertido. El objetivo del presente estudio es analizar el valor del ganglio centinela en los tumores de mama avanzados que han recibido neoadyuvancia y estudiarlos con técnicas de hematoxilina y eosina, inmunohistoquímica y molecular de reacción en cadena de la polimerasa (RT-PCR. Material y métodos. Un total de 33 pacientes fueron incluidos en el estudio; estos enfermos recibieron neoadyuvancia y se les realizó la técnica del ganglio centinela. Una vez extraído el ga nglio, este era enviado a anatomía patológica, donde era evaluado y posteriormente se fragmentaba, utilizando entre un 25% y 50% del ganglio para el estudio en el laboratorio de biología molecular. Resultados. Los ganglios centinelas se estudiaron con las técnicas de hematoxilina y eosina, inmunohistoquímica y la molecular, encontrando un incremento de la sensibilidad con la última técnica, la del RT-PCR. Los ganglios centinelas fueron negativos en un 45.1% y las linfadenectomías fueron negativas en un 61.3% después de neoadyuvancia. Las recidivas locales a 5 años equivalen al 8.3%. Para nuestra serie la tasa de sobrevida libre de enfermedad a 5 años en estadio II es del 100% y en estadio III es del 87.5%. Conclusión. Existe un escaso número de pacientes estudiadas, sin un seguimiento que avale el uso de la biopsia del ganglio centinela después de neoadyuvancia, por lo que actualmente sólo debiera realizarse con fines de investigación. Al comparar las técnicas de hematoxilina y eosina, inmunohistoqu
A prospective randomized study of postoperative adjuvant chemo-radiotherapy (CT+RT) vs. radiotherapy(RT) alone in resected stage II and IIIA non-small cell lung cancer (NSCLC)
International Nuclear Information System (INIS)
Chang, Geol Lee; Joo, Hang Kim; Kyung, Young Chung; Doo, Yun Lee; Kil, Dong Kim; Won, Young Lee; Sung, Kyu Kim; Sei, Kyu Kim; Gwi, Eon Kim
1995-01-01
Objective: A prospective randomized study has been conducted to compare the results of treatment between CT+RT and RT alone as an adjuvant setting in completely resected stage II and IIIA NSCLC. Materials and Methods: Patients who had completely resected stage II and IIIA NSCLC were randomized into a CT+RT arm(arm A) and a RT alone arm(arm B) as an adjuvant setting after stratification according to cell type(squamous vs. non-squamous) and stage(II vs. IIIA). CT(Etoposide 100mg/m2 I.V. infusion d1-3, Cisplatin 20mg/m2 I.V. infusion d1-5, total 6cycles) was started in postop. 3 weeks with a 4 weeks interval. RT(5040cGy/5-6wks, 180cGy/fr) was started in postoperative 5 weeks after the first cycle of CT for group A and in postoperative 4 weeks for group B. A total of 69 patients were registered from Sep. 1990 to Jun. 1993. Sixty five of these patients were evaluable because 4 patients were ineligible due to distant metastasis before adjuvant treatment. Two patients who refused adjuvant treatment were included in this study to avoid selection bias. Results: Sixteen patients (48%) have received CT of more than 3 cycles and 51 patients(78%) have received RT of more than 50Gy. Four patients died due to treatment-related complications [broncho-pleural fistula 3(arm A:B=2:1), pneumonia 1(arm A)]. Survival and the patterns of failure are as follows: Conclusion: There is no statistical significance in either the overall survival or the patterns of failure between the CT+RT arm and RT alone arm as an adjuvant setting in resected stage II and IIIA NSCLC
EVOLUCION DE LA NATALIDAD Y DEL RIESGO REPRODUCTIVO EN MUJERES DE 40 O MAS AÑOS EN LA DECADA DE LOS 90
OpenAIRE
Donoso S., Enrique; Becker V., Jorge; Villarroel del P., Luis
2002-01-01
Se evalúa el riesgo reproductivo en mujeres chilenas de 40 o más años, comparadas con mujeres de 20 a 34 años, en el período 1990 _ 1999. El riesgo se evaluó según Odds Ratio con un intervalo de confianza del 95%. El riesgo de muerte materna (4,05; 3,13-5,23), fetal tardía (2,10; 1,94-2,27), neonatal (1,62; 1,50-1,75) e infantil (1,60; 1,51-1,70) fue más alto en las mujeres mayores. En el grupo estudio, los nacimientos aumentaron significativamente, mientras que en el grupo control el descens...
DeepRT: deep learning for peptide retention time prediction in proteomics
OpenAIRE
Ma, Chunwei; Zhu, Zhiyong; Ye, Jun; Yang, Jiarui; Pei, Jianguo; Xu, Shaohang; Zhou, Ruo; Yu, Chang; Mo, Fan; Wen, Bo; Liu, Siqi
2017-01-01
Accurate predictions of peptide retention times (RT) in liquid chromatography have many applications in mass spectrometry-based proteomics. Herein, we present DeepRT, a deep learning based software for peptide retention time prediction. DeepRT automatically learns features directly from the peptide sequences using the deep convolutional Neural Network (CNN) and Recurrent Neural Network (RNN) model, which eliminates the need to use hand-crafted features or rules. After the feature learning, pr...
Comportamiento de la susceptibilidad magnética en el ambiente ofiolítico del nordeste de Cuba oriental
Directory of Open Access Journals (Sweden)
José A. Batista-Rodríguez
2009-03-01
Full Text Available Se analiza el comportamiento de la susceptibilidad magnética en las rocas de ambiente ofiolítico del nordeste de Cuba oriental con el propósito de esclarecer, en esta zona, la naturaleza geológica de las anomalías del campo magnético; para ello se consideraron, primeramente, resultados de investigaciones petrofísicas anteriores que incluyeran la susceptibilidad magnética; posteriormente, en dos etapas de muestreo separadas por un intervalo de 5 años, se midió esta propiedad en un total de 374 muestras. El estudio permitió conocer que la susceptibilidad magnética constituye un reflejo de la composición e historia geológica de las diferentes rocas de la región, e inferir variaciones en el grado de serpentinización de las rocas ultramáficas, así como el enriquecimiento en minerales magnéticos en todas las rocas estudiadas. Las peridotitas serpentinizadas y los diques de gabros presentan los valores más altos de susceptibilidad magnética reflejando, para las peridotitas, su grado de serpentinización en una relación directa. Ambos grupos de rocas son los principales responsables del comportamiento del campo magnético, el cual parece estar vinculado a las características geométricas y físicas de las peridotitas y algunos diques de gabros.
Duración del periodo de lluvias y aptitud de tierras para la agricultura de temporal / Duration of the rain period and land suitability for rainfed agriculture
Directory of Open Access Journals (Sweden)
Carmen Delgado
2017-09-01
Full Text Available . El concepto duración del periodo de lluvias (DPLL es una propuesta de mejoramiento de otro denominado longitud del periodo de crecimiento (LPC. El objetivo fue realizar un mapa de aptitud para la agricultura de temporal basado en la selección de un modelo de la duración del periodo de lluvias con el índice de humedad. Se utilizaron los datos de 65 estaciones meteorológicas del estado de Yucatán y zonas circundantes. Se analizó la relación entre el resultado del modelo con el mapa de la vegetación potencial. Se encontró que el índice de humedad es mayor cuando proviene del método de Thornthwaite (MT, con el que se obtuvo el mejor modelo cartográco con valor de denición de un mes húmedo cuando es mayor a 0.65. El variograma fue tipo Gaussiano con una r2 = 0.98 y el porcentaje de la varianza explicada C/(C0+C = 74 %. La validación cruzada de la interpolación presentó una r= 0.63 y la raíz cuadrada del error cuadrático medio (RECM = 1.34. Los cinco intervalos de meses húmedos de interés agrícola fue de tres a cuatro meses, de cuatro a cinco meses, de cinco a seis meses, de seis a siete meses, y de siete a ocho meses
Pärt, Arvo. Fratres / Rainer Wagner
Index Scriptorium Estoniae
Wagner, Rainer
1995-01-01
Uuest heliplaadist "Pärt, Arvo. Fratres, Cantus (in Memory of Benjamin Britten), summa, Festina Lente; Peter Manning, France Springuel, Mireille Gleizes, Huub Righarts, I Fiamminghi, Rudolf Werthen" (AD: 1994) Telarc/in-akustik CD 80 387 (WD: 79'00")
Determinação dos intervalos de referência para lipídeos e lipoproteínas em escolares de 10 a 19 anos de idade de Maracaí (SP
Directory of Open Access Journals (Sweden)
Seki Mario
2003-01-01
Full Text Available INTRODUÇÃO: Os estudos epidemiológicos do perfil lipídico em jovens fornecem subsídios para a prevenção da aterosclerose e a redução das elevadas taxas de mortalidade provocadas por doenças do aparelho circulatório. Observa-se, entretanto, que os autores nacionais utilizam intervalos de referência procedentes de outros países, geralmente os norte-americanos. As III Diretrizes Brasileiras Sobre Dislipidemias (III DBSD, 2001 também adotam este recurso, recomendando valores do National Cholesterol Education Program (NCEP (EUA, 1992. Esta prática, que desconsidera diferenças raciais, condições socioeconômicas, constituição física e hábitos alimentares da população brasileira, pode induzir potenciais vieses, prejudicando o dimensionamento das dislipidemias em nosso meio. OBJETIVO: Estabelecer intervalos de referência para triglicerídeos (TG, colesterol total (CT, LDL-colesterol (LDL-c, HDL-colesterol (HDL-c e não-HDL-colesterol (não-HDL-c em escolares de Maracaí (SP, de ambos sexos e faixa etária de 10 a 19 anos. MÉTODOS: Delineamento transversal. No período de 25 de março a 8 de outubro de 2002, foram avaliados 1.371 alunos de ambos os sexos, de 10 a 19 anos de idade, matriculados em oito escolas públicas e uma privada. Foram utilizadas reações enzimáticas para determinações de CT e TG, kits Johnson & Johnson (Ortho Clinical Diagnostics, metodologia de química seca e auto-analisador Vitros 750. A dosagem enzimática de HDL-c foi precedida pela precipitação de LDL-c e VLDL-c presentes nos soros mediante uso de reagentes responsivos à separação magnética, contendo sulfato de dextran e cloreto de magnésio (Johnson & Johnson. Valores de LDL-c para TG sérico abaixo de 400mg/dl foram obtidos pelo cálculo de Friedewald: LDL-c = CT - HDL-c - TG/5; para valores superiores a este, os soros foram precipitados com sulfato de polivinil dissolvido em polietilenoglicol a 25%, pH 6,7 e posterior determinação com
El conocimiento de profesionales de la salud sobre la prevención del cáncer cervical. Alternativas de educación médica
Directory of Open Access Journals (Sweden)
Arillo-Santillán Edna
2000-01-01
Full Text Available OBJETIVO: Evaluar el nivel de conocimientos que diversos tipos de profesionales de la salud tienen sobre la prevención del cáncer cervical. MATERIAL Y MÉTODOS: Se realizó un estudio transversal entre 520 profesionales de la salud del estado de Morelos durante 1998, para evaluar y cuantificar el nivel de conocimientos que poseen en relación con el impacto, la etiología, el tamizaje, el diagnóstico y el tratamiento del cáncer cervicouterino. Se aplicó un cuestionario con escala de 1 a 10, y la muestra incluyó a especialistas en medicina familiar, especialistas de áreas clínicas, enfermeras especialistas y generales, así como trabajadoras sociales. El análisis estadístico incluyó análisis de varianza y estimación de intervalos de confianza al 95%. RESULTADOS: La media del nivel de conocimiento acerca de la prevención del cáncer cervicouterino fue de 4.74, con un intervalo de confianza (IC al 95% de 4.57-4.88, en una escala máxima de 10. La mayor diferencia en el índice de conocimiento acerca de el diagnóstico, la prevención y el tratamiento del cáncer cervical se observó entre los médicos de especialidades clínicas ( o = 5.21, IC95% 4.81-5.60, en comparación con las trabajadoras sociales ( o = 3.07, IC95% 2.31-3.82. En la mayoría de los casos, la periodicidad con la que una mujer debe hacerse la prueba de Papanicolaou se postula menor a un año, y existe un pobre consenso en relación con el periodo de edad en que debe obtenerse dicha prueba. El nivel de conocimientos es menor conforme se tratan de identificar aspectos etiológicos y perspectivas de tratamiento. CONCLUSIONES: La educación médica continua es necesaria para el desarrollo óptimo de un programa de detección oportuna de cáncer cervical. Los resultados del estudio en el estado de Morelos revelan que, además de mejorar los programas académicos de formación de pregrado, es necesario incrementar las intervenciones educativas entre los profesionales de
Estructura de tallas, madurez gonádica y alimentación del pez Diapterus rhombeus (Gerreidae) en el sistema fluvio-deltaico Pom-Atasta, Campeche, México
OpenAIRE
Arturo Aguirre-León; Silvia Díaz-Ruiz
2006-01-01
Se estudió el pez D. rhombeus en el sistema fluviodeltaico Pom-Atasta, asociado a la Laguna de Términos Campeche, en un ciclo anual de 1992 a 1993. Esta especie es dominante en el sistema por su abundancia numérica, peso, frecuencia y amplia distribución. Se obtuvieron 745 individuos con un peso total de 2 890.2 g y un intervalo de longitud total de 3.0 a 16.7 cm. La variación anual del coeficiente alométrico b fue de 2.71 a 3.45. El factor de condición varió de 0.711 a 0.934. Fueron obtenida...
Factores de riesgo del bajo peso al nacer, Hospital Gineco-Obstétrico Provincial de Sancti Spíritus, Cuba
Directory of Open Access Journals (Sweden)
Marcos Aurelio Grau Espinosa
1999-08-01
Full Text Available El objetivo de este trabajo fue identificar algunos factores de riesgo que pudiera presentar la madre durante el embarazo, asociados con la incidencia de bajo peso al nacer (menos de 2500 g. Se diseñó un estudio con 378 casos y 649 controles del Hospital Gineco-Obstétrico Provincial de Sancti Spíritus, Cuba, durante el año 1994. Los datos se obtuvieron de la historia clínica, el registro de partos y una entrevista personal con la madre. Se realizó un análisis bivariado y se controlaron los posibles factores de confusión utilizando la regresión logística dicotómica, mediante los programas Epi Info 5 y SPSS. Por medio del modelo multivariado final se identificaron como factores de riesgo significativos de bajo peso al nacer la hipertensión arterial durante el embarazo, la consulta extemporánea de evaluación del embarazo, los abortos anteriores, último intervalo intergenésico menor de 2 años y el aumento de peso materno menor de 8 kg. Se debe dar prioridad a estas tres últimas variables para mantener buenos indicadores en el programa maternoinfantil de la Provincia de Sancti Spíritus, Cuba.
Comparison of RT-PCR-Dot blot hybridization based on radioisotope 32P with conventional RT-PCR and commercial ELISA Assays for blood screening of HIV-1
International Nuclear Information System (INIS)
Maria Lina R; Andi Yasmon
2011-01-01
There are many commercial ELISA and rapid test kits that have been used for blood screening; however, the kits can give false positive and negative results. Therefore, RT-PCR (Reverse Transcription Polymerase Chain Reaction) - Dot Blot Hybridization based on radioisotope 32 P (RDBR) method was developed in this research, to compare the method with the conventional RT-PCR and commercial ELISA Enzyme-Linked lmmunosorbent Assay) kit. This method is efficient for screening of large blood specimens and surveillance study. Eighty seven samples were used and serum of the samples were tested by ELISA to detect HIV-1. The HIV-l RNA genome was extracted from plasma samples and tested using the RT-PCR and RDBR methods. Of 87 samples that were tested, the rates of positive testing of the RT-PCR, the RDBR, and the ELISA were 71.26%, 74.71%, and 80.46%, respectively. The RDBR (a combination of RTPCR and dot blot hybridization) was more sensitive than conventional RT-PCR by showing 3.45% in increase number of positive specimens. The results showed that of 9 samples (10.34%) were negative RDBR and positive ELISA, while 4 samples (4.60%) were negative ELISA and positive RDBR. The two methods showed slightly difference in the results but further validation is still needed. However, RDBR has high potential as an alternative method for screening of blood in large quantities when compared to method of conventional RT-PCR and ELISA. (author)
Natural and Enhanced Attenuation of Chlorinated Solvents Using RT3D
Energy Technology Data Exchange (ETDEWEB)
Johnson, Christian D.; Truex, Michael J.; Clement, T P.
2006-07-25
RT3D (Reactive Transport in 3-Dimensions) is a reactive transport code that can be applied to model solute fate and transport for many different purposes. This document specifically addresses application of RT3D for modeling related to evaluation and implementation of Monitored Natural Attenuation (MNA). Selection of MNA as a remedy requires an evaluation process to demonstrate that MNA will meet the remediation goals. The U.S. EPA, through the Office of Solid Waste and Emergency Response (OSWER) Directive 9200.4?17P, provides the regulatory context for the evaluation and implementation of MNA. In a complementary fashion, the context for using fate and transport modeling as part of MNA evaluation is described in the EPA?s technical protocol for chlorinated solvent MNA, the Scenarios Evaluation Tool for Chlorinated Solvent MNA, and in this document. The intent of this document is to describe (1) the context for applying RT3D for chlorinated solvent MNA and (2) the attenuation processes represented in RT3D, (3) dechlorination reactions that may occur, and (4) the general approach for using RT3D reaction modules (including a summary of the RT3D reaction modules that are available) to model fate and transport of chlorinated solvents as part of MNA or for combinations of MNA and selected types of active remediation.
Selection of reference genes for quantitative RT-PCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions
DEFF Research Database (Denmark)
Svingen, Terje; Letting, Heidi; Hadrup, Niels
2015-01-01
In biological research the analysis of gene expression levels in cells and tissues can be a powerful tool to gain insights into biological processes. For this, quantitative RT-PCR (RT-qPCR) is a popular method that often involve the use of constitutively expressed endogenous reference (or...... ‘housekeeping’) gene for normalization of data. Thus, it is essential to use reference genes that have been verified to be stably expressed within the specific experimental setting. Here, we have analysed the expression stability of 12 commonly used reference genes (Actb, B2m, Gapdh, Hprt, Pgk1, Rn18s, Rpl13a...
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Milan Kundera poeediparukaga faun / Kärt Hellerma
Index Scriptorium Estoniae
Hellerma, Kärt, 1956-
1996-01-01
Arvustus: Kundera, Milan. Surematus. Tln. : Monokkel, 1995. Ilmunud ka kogumikus: Hellerma, Kärt. Kohanenud kirjandus : valik kirjanduskriitikat 1987-2006. Eesti Keele Sihtasutus : Tallinn, 2006. Lk. 176-179
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WE-H-207B-00: MRgRT
Energy Technology Data Exchange (ETDEWEB)
NONE
2016-06-15
In recent years, steady progress has been made towards the implementation of MRI in external beam radiation therapy for processes ranging from treatment simulation to in-room guidance. Novel procedures relying mostly on MR data are currently implemented in the clinic. This session will cover topics such as (a) commissioning and quality control of the MR in-room imagers and simulators specific to RT, (b) treatment planning requirements, constraints and challenges when dealing with various MR data, (c) quantification of organ motion with an emphasis on treatment delivery guidance, and (d) MR-driven strategies for adaptive RT workflows. The content of the session was chosen to address both educational and practical key aspects of MR guidance. Learning Objectives: Good understanding of MR testing recommended for in-room MR imaging as well as image data validation for RT chain (e.g. image transfer, filtering for consistency, spatial accuracy, manipulation for task specific); Familiarity with MR-based planning procedures: motivation, core workflow requirements, current status, challenges; Overview of the current methods for the quantification of organ motion; Discussion on approaches for adaptive treatment planning and delivery. T. Stanescu - License agreement with Modus Medical Devices to develop a phantom for the quantification of MR image system-related distortions.; T. Stanescu, N/A.
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WE-H-207B-00: MRgRT
International Nuclear Information System (INIS)
2016-01-01
In recent years, steady progress has been made towards the implementation of MRI in external beam radiation therapy for processes ranging from treatment simulation to in-room guidance. Novel procedures relying mostly on MR data are currently implemented in the clinic. This session will cover topics such as (a) commissioning and quality control of the MR in-room imagers and simulators specific to RT, (b) treatment planning requirements, constraints and challenges when dealing with various MR data, (c) quantification of organ motion with an emphasis on treatment delivery guidance, and (d) MR-driven strategies for adaptive RT workflows. The content of the session was chosen to address both educational and practical key aspects of MR guidance. Learning Objectives: Good understanding of MR testing recommended for in-room MR imaging as well as image data validation for RT chain (e.g. image transfer, filtering for consistency, spatial accuracy, manipulation for task specific); Familiarity with MR-based planning procedures: motivation, core workflow requirements, current status, challenges; Overview of the current methods for the quantification of organ motion; Discussion on approaches for adaptive treatment planning and delivery. T. Stanescu - License agreement with Modus Medical Devices to develop a phantom for the quantification of MR image system-related distortions.; T. Stanescu, N/A
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Factores pronósticos del abandono del tratamiento antituberculoso en una región endémica del Perú Predictive factors for noncompliance with tuberculosis treatment in an endemic region of Peru
Directory of Open Access Journals (Sweden)
Dante Roger Culqui
2005-07-01
Full Text Available OBJETIVO: Identificar factores de pronóstico del abandono del tratamiento antituberculoso en la provincia de Ica, Perú. MÉTODOS: Entre 1998 y 2000 se llevó a cabo un estudio de casos y testigos (razón numérica de 1:1 en la provincia de Ica. Se identificaron 55 casos de abandono del tratamiento antituberculoso. Los factores evaluados se seleccionaron a partir del modelo del campo de la salud de Lalonde. Las respectivas razones de posibilidades se calcularon por medio de análisis unifactorial y multifactorial. RESULTADOS: Se identificaron como factores pronósticos del abandono del tratamiento anti-tuberculoso los siguientes: considerar insuficiente la información proporcionada por el personal de salud sobre el tratamiento (razón de posibilidades [odds ratio, OR]: 4,20; intervalo de confianza de 95% [IC95%]: 1,77 a 10,02, considerar inadecuados los horarios para recibir el tratamiento (OR: 9,95; IC95%: 1,97 a 50,21 y consumir drogas ilícitas (OR: 7,15; IC95%: 1,69 a 30,23. CONCLUSIONES: Para mejorar el cumplimiento del régimen antituberculoso es necesario brindar a los pacientes información personalizada sobre la enfermedad y su tratamiento, además de ofrecerles horarios flexibles y apropiados para recibirlo. El consumo de drogas es el factor de riesgo más alto de abandono, por lo que resultan cruciales su identificación y seguimiento.OBJECTIVE: To identify factors that predict noncompliance with tuberculosis treatment in the province of Ica, Peru. METHODS: Between 1998 and 2000 a case-control study (1:1 ratio was conducted in the province of Ica, with 55 cases (persons who dropped out of treatment being identified. The factors evaluated were chosen from Lalonde's model of the field of health. The respective odds ratios were calculated by means of univariate analysis and multivariate analysis. RESULTS: The following factors were identified as being predictive of noncompliance with tuberculosis treatment: thinking that the
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Espaçamento de plantio e intervalos de colheita na biomassa e no óleo essencial de gerânio Plant spacing and harvest intervals on biomass and essential oil of geranium
Directory of Open Access Journals (Sweden)
Arie F Blank
2012-12-01
Full Text Available O gerânio (Pelargonium graveolens é uma erva aromática e seu óleo essencial é comumente utilizado na constituição de fármacos e cosméticos no mundo. A planta adapta-se bem às condições climáticas do nordeste brasileiro, mas são escassos os dados sobre seu rendimento quantitativo e qualitativo. O objetivo do trabalho foi avaliar a influência de espaçamento de plantas e intervalos de colheita em gerânio na produção de biomassa e de óleo essencial. Foram testados três espaçamentos de plantio (50x50, 50x60 e 50x80 cm e três intervalos de colheita (8, 12 e 16 semanas. Os maiores valores totais de massa fresca e seca de folhas e caules (2679,04 g m-2; 424,62 g m-2; 1035,08 g m-2; 136,85 g m-2, respectivamente e rendimento de óleo essencial (7,56 mL m-2, que são características de interesse direto para o mercado, foram obtidos em intervalo de colheita de oito semanas e no espaçamento de 50x50 cm. Nas demais variáveis analisadas, houve pouca diferenciação entre os tratamentos, contudo, períodos longos de colheita são menos produtivos, pois houve queda nos valores de todas as variáveis nas ultimas colheitas de cada intervalo. Assim, melhores resultados de quantidade e qualidade para o gerânio foram obtidos com intervalo de 8 semanas e no espaçamento 50x50 cm.Geranium (Pelargonium graveolens is an aromatic herb and its essential oil is commonly used in the creation of drugs and cosmetics worldwide. The plant is well adapted to the climatic conditions of the Brazilian northeast, but there are few data on its quantitative and qualitative yield. The aim of this study was to evaluate the influence of plant spacing and harvest intervals of geranium on the production of biomass and essential oil. Three different plant spacings (50x50, 50x60 and 50x80 cm and three harvest intervals (8, 12 and 16 weeks were tested. The highest values of total fresh and dry weight of leaves and stems (2679.04 g m-2; 424.62 g m-2; 1035.08 g m-2
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Repartición del recurso trófico entre Mullus barbatus y M. surmuletus en el Mar Catalán (Mediterráneo Noroccidental
Directory of Open Access Journals (Sweden)
2005-01-01
Full Text Available Se examinó la composición trófica de Mullus barbatus y M. surmuletus con el fin de evaluar la repartición del recurso entre estas dos especies simpátricas. Para cada especie, los peces se agruparon en intervalos de clase de 1 cm (longitud total = LT. Se realizó el análisis de agrupamiento utilizando el índice de similitud de Bray-Curtis y el método de agrupación de UPGMA. Previamente, para cada intervalo de clase se estandarizó y transformó a doble raíz cuadrada la abundancia de las presas. Con base en la similitud trófica, se definieron seis nuevos grupos: (I adultos mayores de M. surmuletus (21–31 cm LT, (II organismos recién asentados de ambas especies (6–8 cm LT, (III adultos mayores de M. barbatus (19–21 cm LT, (IV adultos de M. barbatus (12–18 cm LT, (V juveniles de ambas especies y (VI adultos de M. surmuletus (12–20, 22 cm LT. El análisis de similitud (ANOSIM muestra que existen diferencias significativas entre la composición trófica de los grupos sucesivos (P ≤ 0.05. Si bien estas especies simpátricas consumen una amplia gama de presas en común, la proporción relativa de sus componentes muestra diferencias claras entre especies e incluso dentro de cada especie a lo largo de su desarrollo; estas diferencias se tornan más evidentes en los organismos mayores. Los resultados del presente trabajo resaltan la importancia de la repartición trófica como un mecanismo que facilita la coexistencia intra e inter específica de estas dos especies simpátricas.
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Macrocrustáceos (Peracarida, Decapoda de fondos carbonatados del sector occidental del banco de Campeche en el sur del golfo de México Macrocrustacea (Peracarida, Decapoda from carbonated habitats in the western sector of the Campeche Bank in the Southern Gulf of Mexico
Directory of Open Access Journals (Sweden)
Elva Escobar-Briones
2010-10-01
Full Text Available Este estudio reconoce el número de familias, abundancia, diversidad biológica y distribución de las comunidades de macrocrustáceos asociadas a fondos carbonatados del sector occidental del banco de Campeche. Las muestras analizadas de fondos carbonatados provinieron de colectas realizadas a bordo del B/O Pelican de la Universidad de Louisiana con arrastres efectuados en un intervalo de 20 a 182 m de profundidad. Se identificaron 64 familias. El número de familias, la abundancia y diversidad biológica disminuyó con la profundidad. La distancia a los bancos y arrecifes determinan la variación en la riqueza de familias y la distribución de la asociación de macrocrustáceos. El banco de Campeche puede considerarse un hotspot de diversidad biológica marina para los macrocrustáceos asociados a fondos carbonatados.This study contributes with information on the number of families, abundance, biodiversity and geographical distribution of the crustacean assemblage associated to carbonated habitats in the western sector of the Campeche Bank. The samples studied were collected onboard the R/V Pelican of the University of Louisiana from dredge materials obtained at a range of 20 to 182 m depth. The specimens were grouped into 64 families. The number of families, the abundance and diversity values decreased with increasing depth. The distance to the banks and reef islands determined the variability recorded in the richness of families and the distribution patterns of the macrocrustacean assemblage. The Campeche Bank is suggested to be a "hotspot" of biological diversity for the macrocrustaceans associated to the carbonated bottoms.
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Quantitative real-time RT-PCR and chromogenic in situ hybridization
DEFF Research Database (Denmark)
Rosa, Fabíola E; Silveira, Sara M; Silveira, Cássia G T
2009-01-01
. METHODS: To elucidate the molecular profile of HER-2 status, mRNA and protein expression in 75 invasive breast carcinomas were analyzed by real time quantitative RT-PCR (qRT-PCR) and IHC, respectively. Amplifications were evaluated in 43 of these cases by CISH and in 11 by FISH. RESULTS: The concordance...
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Direct recovery of infectious Pestivirus from a full-length RT-PCR amplicon
DEFF Research Database (Denmark)
Rasmussen, Thomas Bruun; Reimann, Ilona; Hoffmann, Bernd
2008-01-01
This study describes the use of a novel and rapid long reverse transcription (RT)-PCR for the generation of infectious full-length cDNA of pestiviruses. To produce rescued viruses, full-length RT-PCR amplicons of 12.3 kb, including a T7-promotor, were transcribed directly in vitro, and the result......This study describes the use of a novel and rapid long reverse transcription (RT)-PCR for the generation of infectious full-length cDNA of pestiviruses. To produce rescued viruses, full-length RT-PCR amplicons of 12.3 kb, including a T7-promotor, were transcribed directly in vitro......, and the resulting RNA transcripts were electroporated into ovine cells. Infectious virus was obtained after one cell culture passage. The rescued viruses had a phenotype similar to the parental Border Disease virus strain. Therefore, direct generation of infectious pestiviruses from full-length RT-PCR cDNA products...
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Comparación del efecto citotóxico de tres agentes quelantes sobre fibroblasto del ligamento periodontal humano. Estudio in vitro
Directory of Open Access Journals (Sweden)
Edwin De la Cruz Rocha
2012-01-01
Full Text Available Objetivo: Comparar in vitro el efecto citotóxico de tres agentes quelantes sobre fibroblastos del ligamento periodontal humano. Materiales y métodos: Se utilizaron cultivos de fibroblastos de ligamento periodontal humano, los cuales fueron colocados en contacto con los agentes quelantes a evaluar (RCPrep, Glyde, EDTA al 17% a intervalos de 15, 30 y 60 minutos. Se midió la absorbancia para cada uno de los grupos, para determinar el grado de actividad enzimática, que es indicador de muerte celular. Previo a la cuantificación de la absorbancia se corroboró microscópicamente la formación de los cristales de formazán, los cuales se forman alrededor de los fibroblastos, y su presencia es indicador de integridad de la membrana y de la actividad metabólica. Por microscopia se verificó la formación de cristales de formazan, después de agregar azul de tripán. Resultados: El Glyde mostró mayor grado de citotoxicidad, con una diferencia estadísticamente significativa, al compararlo con EDTA 17% y el RC-PREP. El EDTA presentó mayor citotoxicidad que el RC-PREP a los 15minutos, evento que cambió a los 30 y 60 minutos. Conclusiones: Los agentes quelantes RC-Prep, Glyde y EDTA tienen un efecto citotóxico a nivel de los fibroblastos del ligamento periodontal, siendo el EDTA el de menor efecto citotóxico a los 30 y 60 minutos comparado con RC-Prep y Glyde.
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Effect of low frequency ultrasound on combined rt-PA and eptifibatide thrombolysis in human clots.
Science.gov (United States)
Meunier, Jason M; Holland, Christy K; Pancioli, Arthur M; Lindsell, Christopher J; Shaw, George J
2009-01-01
Fibrinolytics such as recombinant tissue plasminogen activator (rt-PA) are used to treat thrombotic disease such as acute myocardial infarction (AMI) and ischemic stroke. Interest in increasing efficacy and reducing side effects has led to the study of adjuncts such as GP IIb-IIIa inhibitors and ultrasound (US) enhanced thrombolysis. Currently, GP IIb-IIIa inhibitor and fibrinolytic treatment are often used in AMI, and are under investigation for stroke treatment. However, little is known of the efficacy of combined GP IIb-IIIa inhibitor, fibrinolytic and ultrasound treatment. We measure the lytic efficacy of rt-PA, eptifibatide (Epf) and 120 kHz ultrasound treatment in an in-vitro human clot model. Blood was drawn from 15 subjects after IRB approval. Clots were made in 20 microL pipettes, and placed in a water tank for microscopic visualization during lytic treatment. Clots were exposed to control, rt-PA (rt-PA), eptifibatide (Epf), or rt-PA+eptifibatide (rt-PA + Epf), with (+US) or without (-US) ultrasound for 30 minutes at 37 degrees C in human plasma. Clot lysis was measured over time, using a microscopic imaging technique. The fractional clot loss (FCL) and initial lytic rate (LR) were used to quantify lytic efficacy. LR values for (- US) treated clots were 0.8+/-0.1(control), 1.8+/-0.3 (Epf), 1.5+/-0.2 (rt-PA), and 1.3+/-0.4 (rt-PA + Epf) (% clot width/minute) respectively. In comparison, the (+ US) group exhibited LR values of 1.6+/-0.2 (control), 4.3+/-0.4 (Epf), 6.3+/-0.4 (rt-PA), and 4.6+/-0.6 (rt-PA + Epf). For (- US) treated clots, FCL was 6.0+/-0.8 (control), 9.2+/-2.5 (Epf), 15.6+/-1.7 (rt-PA), and 28.0+/-2.2% (rt-PA + Epf) respectively. FCL for (+ US) clots was 13.5+/-2.4 (control), 20.7+/-6.4 (Epf), 44.4+/-3.6 (rt-PA) and 30.3+/-3.6% (rt-PA + Epf) respectively. Although the addition of eptifibatide enhances the in-vitro lytic efficacy of rt-PA in the absence of ultrasound, the efficacy of ultrasound and rt-PA is greater than that of combined
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Pärt, Arvo: "Litany" / Patric Wiklacz
Index Scriptorium Estoniae
Wiklacz, Patric
1996-01-01
Uuest heliplaadist "Pärt, Arvo: "Litany". Litany. Psalom. Trisagion. The Hilliard Ensemble, Orchestre de chambre de Tallinn, Choeur de chambre Philharmonique d'Estonie, Tõnu Kaljuste. Orchestre de chambre de Lituanie, Saulius Sondeckis. ECM New Series ECM 1592, distribution Polygram 449 810-2 (CD:158F)
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Pärt, Arvo: "Litany" / Barry Witherden
Index Scriptorium Estoniae
Witherden, Barry
1996-01-01
Uuest heliplaadist "Pärt, Arvo: "Litany". Litany. Psalom. Trisagion. The Hilliard Ensemble, Tallinn Chamber Orchestra, Estonian Philharmonic Chamber Orchestra, Estonian Philharmonic Chamber Choir, Tõnu Kaljuste. Orchestre de chambre de Lituanie, Saulius Sondeckis". ECM New Series ECM 1592, distribution Polygram 449 810-2 (CD:158F)
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Análisis de las dependencias composicionales de las propiedades opticas de láminas semiconductoras amorfas del sistema As-S-Se
Directory of Open Access Journals (Sweden)
González-Leal, J. M.
2004-04-01
Full Text Available Amorphous As40S60-xSex (x = 0, 20, 30, 40 and 60 at. % films have been prepared by vacuum thermal evaporation. Values for the refractive index and the absorption coefficient of these films have been determined from their transmission spectra, measured at normal incidence, in the spectral range from 400 to 2200 nm. It has been found that the refractive index of the samples increases with increasing Se content, over the entire spectral range. This behaviour can be explained, according to the Lorentz-Lorenz relationship, by the increased polarizability of the larger Se atoms, in comparison with S atoms. The values of the As effective coordination number, Nc, have been estimated from the analysis of the dispersion of the refractive index, in terms of a model based on the single-oscillator formula. The increase in Nc with increasing Se content, from around 3.0 to 3.4, which has been inferred from this analysis, suggests an increase in the structural compactness of the films when substituting S by Se. This conclusion is supported by the decrease observed in the intensity of the first sharp diffraction peak, in the X-ray patterns. Finally, it has been found a decrease in the optical bandgap with increasing Se content, from 2.38 to 1.79 eV, which can be plausibly explained considering the lower bonding energy of As-Se bonds compared with that of As-S bonds.
Se han preparado láminas semiconductoras amorfas de composiciones químicas As40S60-xSex (con x = 0, 20, 30, 40 y 60 % at., mediante la técnica de evaporación térmica en vacío. Los valores del índice de refracción y del coeficiente de absorción de estas láminas, se determinaron a partir de los espectros de transmisión óptica, obtenidos en incidencia normal, en el intervalo espectral comprendido entre 400 y 2200 nm. Se ha encontrado un aumento en los valores del índice de refracción con el contenido de Se, dentro del intervalo espectral en estudio. El análisis de la dependencia
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The SPoRT-WRF: Evaluating the Impact of NASA Datasets on Convective Forecasts
Science.gov (United States)
Zavodsky, Bradley; Kozlowski, Danielle; Case, Jonathan; Molthan, Andrew
2012-01-01
Short-term Prediction Research and Transition (SPoRT) seeks to improve short-term, regional weather forecasts using unique NASA products and capabilities SPoRT has developed a unique, real-time configuration of the NASA Unified Weather Research and Forecasting (WRF)WRF (ARW) that integrates all SPoRT modeling research data: (1) 2-km SPoRT Sea Surface Temperature (SST) Composite, (2) 3-km LIS with 1-km Greenness Vegetation Fraction (GVFs) (3) 45-km AIRS retrieved profiles. Transitioned this real-time forecast to NOAA's Hazardous Weather Testbed (HWT) as deterministic model at Experimental Forecast Program (EFP). Feedback from forecasters/participants and internal evaluation of SPoRT-WRF shows a cool, dry bias that appears to suppress convection likely related to methodology for assimilation of AIRS profiles Version 2 of the SPoRT-WRF will premier at the 2012 EFP and include NASA physics, cycling data assimilation methodology, better coverage of precipitation forcing, and new GVFs
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Teekond Kirjasõna Vabariigis / Märt Väljataga ; intervjueerinud Marek Tamm
Index Scriptorium Estoniae
Väljataga, Märt, 1965-
2015-01-01
Märt Väljataga tõlgitud R. Rorty artiklist "Pragmatism ja filosoofia", mis ilmus Akadeemia 1992, nr. 2, lk. 281-282, 285, tema filosoofiast ja Märt Väljataga tööst ajakirja "Vikerkaar" toimetajana ning tõlkijana
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TRASTORNOS DEL ESPECTRO AUTISTA Y EXPOSICIONES OCUPACIONALES DE LOS PROGENITORES
Directory of Open Access Journals (Sweden)
Manuel Pino-López
2013-01-01
Full Text Available Fundamentos: Estudios con hermanos y gemelos sugieren un componente genético en el origen del autismo que no explica su crecimiento actual. El objetivo es investigar si factores ambientales como algunas exposiciones profesionales (trabajo nocturno, manejo de disolventes y/o campos electromagnéticos incrementan la probabilidad de trastornos del espectro autista (TEA en los hijos. Métodos: Estudio observacional de casos y controles mediante análisis de expedientes de 206 niños entre 16 y 36 meses de edad evaluados en el Servicio de Atención Temprana de Ciudad Real (70 con TEA y 136 no afectados. Para medir el riesgo de TEA asociado al trabajo nocturno, con disolventes y/o campos electromagnéticos se calculó la odds ratio (OR con un intervalo de confianza (IC del 95%. Resultados: El riesgo de TEA se multiplica por 2,22 cuando un progenitor trabaja en las ocupaciones estudiadas (OR=2,22, IC 95%=1,42-3,48, destacando trabajo con disolventes (OR=2,81, IC 95%=1,28-6,17 y nocturno (OR=2,18, IC 95%=1,21-3,93. El riesgo se multiplica por 3 si la madre trabaja en estas ocupaciones (OR=3, IC95%=1,44-6,26, destacando trabajo nocturno (OR=3,47, IC 95%=1,39-8,63 y con disolventes (OR=2,88, IC 95%=1,28-6,17. El riesgo se multiplica por 1,94 si el padre trabaja en estas ocupaciones(OR=1,94, IC 95%=1,07-3,53 y por 2,81 con disolventes (OR=2,81, IC 95%=1,01-7,86. Se encontró asociación positiva entre nivel educativo de los progenitores y TEA. Conclusiones: Encontramos relación significativa entre exposición de los progenitores a los riesgos estudiados y TEA en los hijos. Los resultados sugieren la participación de alteraciones genéticas ocasionadas por factores ambientales en el origen del trastorno.
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Factors influencing the use of RT in NSW: a qualitative study exploring consumer and health professional practices
International Nuclear Information System (INIS)
Sundaresan, Puma; Milross, Christopher G.; Stockler, Martin R.; Smith, Andrea; Evans, Alison; King, Madeleine T.
2014-01-01
Radiotherapy (RT) is an essential and cost-effective cancer treatment. It is underutilised in Australia. Bridging the gap between actual and optimal RT utilisation requires not only provision of adequate RT infrastructure but also an understanding of the factors that influence the extent to which this opportunity for RT is utilised. This study explored factors perceived to affect RT-related decision making by consumers and health professionals (HPs). Six semi-structured focus groups (FGs) and 13 interviews were conducted at three geographical locations in NSW, Australia (n=26 consumers and 30 HPs). Audio recordings of FGs and interviews were transcribed verbatim and analysed thematically. An exhaustive list of issues perceived to affect consumer and HP RT decisions was identified. There were common themes across participant groups and locations. Perceptions of RT and its benefits, as well as accurate communication of the expected benefits and risks of RT, were highlighted as important to decision making. Perceived factors relating to 'inconvenience' of RT were multifaceted and included travel, relocation, accommodation, time away from work and financial challenges. Perceived potential barriers to RT referral included knowledge of RT and RT services, availability of a local or visiting RT service, referrer bias, and the low profile of RT. Important drivers during RT decisions appear to include the perceived benefit, risks and inconvenience of RT. Underutilisation of RT may also result from multiple barriers at the referrer level. Further research into whether these factors influence actual RT decisions is needed.
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Temperature affects thrombolytic efficacy using rt-PA and eptifibatide, an in vitro study.
Science.gov (United States)
Meunier, Jason M; Chang, Wan-Tsu W; Bluett, Brent; Wenker, Evan; Lindsell, Christopher J; Shaw, George J
2012-09-01
The potential for hypothermia as a neuroprotectant during stroke has led to its increase in clinical use. At the same time, combination pharmaceutical therapies for ischemic stroke using recombinant tissue plasminogen activator (rt-PA), and GP IIb-IIIa inhibitors, such as Eptifibatide (Epf ), are under study. However, there is little data on how the reactions triggered by these agents are impacted by temperature. Here, clot lysis during exposure to the combination of rt-PA and Epf is measured in an in vitro human clot model at hypothermic temperatures. The hypothesis is that lytic efficacy of rt-PA and Epf decreases with decreasing temperature. Whole blood clots from 31 volunteers were exposed to rt-PA (0.5 μg/mL) and Epf (0.63 μg/mL) in human fresh-frozen plasma (rt-PA+Epf ), rt-PA alone in plasma (rt-PA Alone), or to plasma alone (Control), at temperatures from 30°C to 37°C, for 30 minutes. Clot lysis was measured using a microscopic imaging technique; the mean fractional clot loss (FCL) at 30 minutes was used to determine lytic efficacy. Temperature had a significant impact on FCL in clots exposed to rt-PA+Epf, with the FCL being lower at 30°C to 36°C than at 37°C. The FCL remained significantly higher for rt-PA+Epf–treated clots than Controls regardless of temperature, with the exception of measurements made at 30°C when no significant differences in the FCL were observed between groups. The use of hypothermia as a neuroprotectant may negatively impact the therapeutic benefit of thrombolytic agents.
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Development and implementation of the quality control panel of RT-PCR and real-time RT-PCR for avian influenza A (H5N1 surveillance network in mainland China
Directory of Open Access Journals (Sweden)
Wang Wei
2011-03-01
Full Text Available Abstract Background Reverse transcription PCR (RT-PCR and real time RT-PCR (rRT-PCR have been indispensable methods for influenza surveillance, especially for determination of avian influenza. The movement of testing beyond reference lab introduced the need of quality control, including the implementation of an evaluation system for validating personal training and sample proficiency testing. Methods We developed a panel with lysates of seasonal influenza virus (H1N1, H3N2 and B, serials of diluted H5N1 virus lysates, and in-vitro transcribed H5 hemaglutinin (HA and an artificial gene RNAs for RT-PCR and rRT-PCR quality control assessment. The validations of stability and reproducibility were performed on the panel. Additionally, the panel was implemented to assess the detection capability of Chinese human avian influenza networks. Results The panel has relatively high stability and good reproducibility demonstrated by kappa's tests. In the implementation of panel on Chinese human avian influenza networks, the results suggested that there were a relatively low number of discrepancies for both concise and reproducibility in Chinese avian influenza virus net works. Conclusions A quality control panel of RT-PCR and real-time RT-PCR for avian influenza A (H5N1 surveillance network was developed. An availably statistical data, which are used to assess the detection capability of networks on avian influenza virus (H5N1, can be obtained relatively easily through implementation of the panel on networks.
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Caracterización etiológica del síndrome de Lennox-Gastaut sintomático
Directory of Open Access Journals (Sweden)
Ileana Valdivia Álvarez
2012-03-01
Full Text Available Introducción: el síndrome de Lennox-Gastaut es una encefalopatía epiléptica dependiente de la edad, de gran severidad, por su farmacorresistencia y las discapacidades asociadas. Objetivos: caracterizar el síndrome de Lennox-Gastaut sintomático según sexo, edad de inicio y diagnóstico, tipo de crisis predominantes, hallazgos en neuroimagen y etiología. Métodos: estudio descriptivo, retrospectivo con una muestra de 36 pacientes egresados del servicio de neuropediatría del Hospital Pediátrico "Juan M. Márquez", con diagnóstico de síndrome de Lennox-Gastaut sintomático y que tuvieran estudios de neuroimagen (tomografía axial computarizada y resonancia magnética nuclear. Las variables cualitativas se describieron estadísticamente mediante frecuencias absolutas y cifras porcentuales. Para los porcentajes de interés, se calculó su intervalo de confianza con el 95 % de confiabilidad (IC 95 %. Resultados y conclusiones: el síndrome de Lennox-Gastaut sintomático fue más frecuente en varones, que comenzaron con epilepsia antes del año de edad, el 33 % presentó síndrome de West. Más del 60 % se diagnosticó antes de los 4 años de edad. Las crisis más frecuentes fueron las tónicas y atónicas de cuello. La tomografía axial computarizada permite localizar zonas de atrofia y pocas lesiones estructurales, que se precisan por resonancia magnética nuclear como alteraciones de la migración neuronal. Después de las malformaciones del sistema nervioso central, la causa más frecuente fue la hipoxia perinatal.
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A study on the establishment of practical program for the RT promotion plan
Energy Technology Data Exchange (ETDEWEB)
Kim, Seong Yen; Jin, J. H.; Beyn, M. W.; Han, H. S.; Park, C.; Lee, J. W.; Yang, M. S.; Jeong, I. H.; Nho, Y. C
2003-03-01
The present state of RT utilizations in Korea is categorized into radioisotope applications to treat incurable diseases, food irradiation, and nondestructive test, and radiation applications by radiation generators such as cyclotrons to produce short half-life radioisotopes for medical uses and electron accelerators to make better products of cables and radial tiers. In order to activate more utilizations of RT, the followings should be carried out: 1. Establishment of RT Research Institute and RT Industrial Complex - Establishment of the tentatively named [Radiation Science and Technology Research Center] and 'Cyclotron Center' in four provinces should be effectively carried out as scheduled. - Successful pursuing design and construction of the proton accelerator for research and technology development, and CNS research facility. - Radiation area workers training participating the RT projects; Participation and training of university graduates to the on-going and planned RT projects, Classes for special skills and experts to radiation could be designed at the Consociated Graduate School, being established by joining national research organizations, if necessary. - Secure minimum number of R and D man power for RT development 2. Review design and construction of RI production reactor and construct distribution and transportation of RIs - Constructions of distribution lines and a central depository site with the establishment of Cyclotron Centers and RI production reactor - Designation, as early as possible, of the organization to handle the constructions of the distribution lines and the central depository site. 3. Establishment of an organization to appeal RT industries' concerns - An organization composed of governmental officials, researchers from national research institutes and/or private research institutes, representatives from associations and RT industries should be formed to define the issues to promote radiation uses. 4. Establishment of
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A study on the establishment of practical program for the RT promotion plan
International Nuclear Information System (INIS)
Kim, Seong Yen; Jin, J. H.; Beyn, M. W.; Han, H. S.; Park, C.; Lee, J. W.; Yang, M. S.; Jeong, I. H.; Nho, Y. C.
2003-03-01
The present state of RT utilizations in Korea is categorized into radioisotope applications to treat incurable diseases, food irradiation, and nondestructive test, and radiation applications by radiation generators such as cyclotrons to produce short half-life radioisotopes for medical uses and electron accelerators to make better products of cables and radial tiers. In order to activate more utilizations of RT, the followings should be carried out: 1. Establishment of RT Research Institute and RT Industrial Complex - Establishment of the tentatively named [Radiation Science and Technology Research Center] and 'Cyclotron Center' in four provinces should be effectively carried out as scheduled. - Successful pursuing design and construction of the proton accelerator for research and technology development, and CNS research facility. - Radiation area workers training participating the RT projects; Participation and training of university graduates to the on-going and planned RT projects, Classes for special skills and experts to radiation could be designed at the Consociated Graduate School, being established by joining national research organizations, if necessary. - Secure minimum number of R and D man power for RT development 2. Review design and construction of RI production reactor and construct distribution and transportation of RIs - Constructions of distribution lines and a central depository site with the establishment of Cyclotron Centers and RI production reactor - Designation, as early as possible, of the organization to handle the constructions of the distribution lines and the central depository site. 3. Establishment of an organization to appeal RT industries' concerns - An organization composed of governmental officials, researchers from national research institutes and/or private research institutes, representatives from associations and RT industries should be formed to define the issues to promote radiation uses. 4. Establishment of Pan-ministry PR
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Tabaquismo en médicos y en la población general. Comparación de los resultados del estudio TAMARA y de la Encuesta Nacional de Factores de Riesgo 2005
Directory of Open Access Journals (Sweden)
Daniel Ferrante
2008-01-01
Full Text Available Aunque se demostró la influencia del médico sobre el paciente fumador para desalentar y controlar la adicción, esta actitud depende en gran medida de la relación del propio médico con el tabaco. El objetivo de este estudio fue comparar la prevalencia de tabaquismo en médicos del estudio TAMARA (Tabaquismo en médicos de la República Argentina versus la comunicada para la población general en la Encuesta Nacional de Factores de Riesgo 2005 (ENFR.Se recurrió a la base de datos del estudio TAMARA para estratificar la población de médicos de acuerdo con los intervalos de edad comunicados por la ENFR. De los 6.497 médicos originales del TAMARA, se incluyeron 5.806 en el intervalo de 25 a 64 años, mientras que de la ENFR se utilizaron los datos de 9.658 encuestados de la población general. Se encontraron prevalencias de fumadores similares para los médicos y la población general (32,2% versus 32,4%; p = 0,403; sin embargo, al estratificar por edad se demostró un patrón distinto. La proporción de médicos fumadores fue inferior a la de la población general entre los 25 y los 34 años (30,9% versus 34,6%; p = 0,0004 y entre los 50 y los 64 años (21,3% versus 26,8%; p < 0,0001, mientras que en el grupo de edad intermedia (35- 49 años el porcentaje de médicos fumadores superó la prevalencia de la ENFR (44,4 versus 35,8%; p < 0,0001. Se observó además que este estrato de edad intermedia fue el que tenía menos formación para el manejo del tabaquismo.En conclusión, si bien la prevalencia global del tabaquismo en médicos no es diferente de la correspondiente a la población general, la estratificación por edad mostró que los médicos eran más fumadores entre los 35 y los 49 años. Aunque en los grupos de menor y mayor edad se encontraron menos fumadores entre los médicos, esta diferencia no fue tan marcada como en el grupo intermedio. La alta prevalencia de fumadores entre los médicos podría constituirse en una influencia
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Variance to mean ratio, R(t), for poisson processes on phylogenetic trees.
Science.gov (United States)
Goldman, N
1994-09-01
The ratio of expected variance to mean, R(t), of numbers of DNA base substitutions for contemporary sequences related by a "star" phylogeny is widely seen as a measure of the adherence of the sequences' evolution to a Poisson process with a molecular clock, as predicted by the "neutral theory" of molecular evolution under certain conditions. A number of estimators of R(t) have been proposed, all predicted to have mean 1 and distributions based on the chi 2. Various genes have previously been analyzed and found to have values of R(t) far in excess of 1, calling into question important aspects of the neutral theory. In this paper, I use Monte Carlo simulation to show that the previously suggested means and distributions of estimators of R(t) are highly inaccurate. The analysis is applied to star phylogenies and to general phylogenetic trees, and well-known gene sequences are reanalyzed. For star phylogenies the results show that Kimura's estimators ("The Neutral Theory of Molecular Evolution," Cambridge Univ. Press, Cambridge, 1983) are unsatisfactory for statistical testing of R(t), but confirm the accuracy of Bulmer's correction factor (Genetics 123: 615-619, 1989). For all three nonstar phylogenies studied, attained values of all three estimators of R(t), although larger than 1, are within their true confidence limits under simple Poisson process models. This shows that lineage effects can be responsible for high estimates of R(t), restoring some limited confidence in the molecular clock and showing that the distinction between lineage and molecular clock effects is vital.(ABSTRACT TRUNCATED AT 250 WORDS)
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Tipificación molecular del virus dengue 3 durante el brote epidémico de dengue clásico en Lima, Perú, 2005
Directory of Open Access Journals (Sweden)
Enrique Mamani Z
2005-07-01
Full Text Available Objetivos: Identificar mediante trascripción reversa-reacción en cadena de la polimerasa (RT-PCR y sitios específicos de restricción - reacción en cadena de la polimerasa (RSS-PCR al agente causal del brote epidémico presentado en el distrito de Comas, Lima en abril del año 2005. Materiales y métodos: veinte muestras de suero colectadas durante el brote de dengue fueron procesados por RT-PCR para determinar el serotipo, esta técnica se realizó en un solo paso. Luego se aplicó la técnica RSS-PCR para la identificación del genotipo circulante y se corroboraron los resultados posteriormente con aislamiento viral y secuenciamiento. Resultados: El análisis del RTPCR del ARN extraído de las muestras presentó un producto amplificado de 290pb que corresponden al dengue serotipo 3 (DEN 3. El análisis de los productos de RSS-PCR del ARN extraído a partir de aislamientos de DEN 3 correspondió al patrón C, incluido en el genotipo III. Los aislamientos de los virus dengue 3 en líneas celulares C6/36, tipificadas por IFI y el secuenciamiento genético confirmaron los resultados obtenidos por las pruebas previamente descritas. Conclusión: Durante el brote epidémico de dengue clásico en Lima, circuló el genotipo III del virus DEN 3.
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El rol mediador del aprendizaje entre el liderazgo y la realización del trabajador
Directory of Open Access Journals (Sweden)
José Angel Miguel-Dávila
2014-02-01
Full Text Available Objeto: Bajo la perspectiva de la Gestión de la Calidad Total (GCT aplicada a entidades gestoras de servicios deportivos, el presente trabajo pretende aportar a la escasa literatura existente, una alternativa a las relaciones clásicas entre sus constructos. En concreto, se estudia la relación entre liderazgo visionario (LV y realización del trabajador (RT y, una vez evaluada ésta, conocer el papel que tiene el aprendizaje organizativo (AO. Diseño/metodología: Cuestionario online a los directivos de los campos de golf de España y Portugal, países importantes de esta oferta turística, obteniéndose una muestra de 132 empresas. Aportaciones y resultados: Respecto al primer objetivo, los resultados muestran una relación directa y significativa del liderazgo visionario con la realización del trabajador. Asimismo, se identifica el aprendizaje organizativo como mediador parcial de la relación anterior; es decir, el líder no incide en los empleados únicamente a través del proceso de aprendizaje, sino que la transmisión de su visión se relaciona también directamente con la realización de los empleados. Limitaciones: La principal de esta investigación es común a otros estudios anteriores: el hecho de que todos los participantes pertenezcan a la misma industria, el turismo deportivo, limita la generalización de resultados a otros sectores. Valor añadido: El primer objetivo que relaciona dos variables del modelo, ya supone una primera contribución, puesto que el estudio de relaciones adicionales al Modelo de Gestión de Deming ha sido escasamente contrastado desde la perspectiva de la GCT. Por otra parte, el contexto donde esta nueva relación es contrastada, campos de golf, ayuda a generar literatura sobre calidad en centros deportivos así como aumentar la aplicabilidad de las relaciones del modelo GCT en el sector servicios.
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RT-PCR for detection of all seven genotypes of Lyssavirus genus.
Science.gov (United States)
Vázquez-Morón, S; Avellón, A; Echevarría, J E
2006-08-01
The Lyssavirus genus includes seven species or genotypes named 1-7. Rabies genotypes correlate with geographical distribution and specific hosts. Co-circulation of different lyssaviruses, imported cases, and the presence of unknown viruses, such as Aravan, Khujand, Irkut and West Caucasian Bat Virus, make it necessary to use generic methods able to detect all lyssaviruses. Primer sequences were chosen from conserved regions in all genotypes in order to optimise a generic RT-PCR. Serial dilutions of 12 RNA extracts from all seven Lyssavirus genotypes were examined to compare the sensitivity of the RT-PCR standardised in this study with a published RT-PCR optimised for EBLV1 detection and capable of amplifying RNA from all seven lyssaviruses. All seven genotypes were detected by both RT-PCRs, however, the sensitivity was higher with the new version of the test. Twenty samples submitted for rabies diagnosis were tested by the new RT-PCR. Eight out of 20 samples from six dogs, one horse and one bat were found positive, in agreement with immunofluorescence results. Seven samples from terrestrial mammals were genotype 1 and one from a bat was genotype 5. In conclusion, this method can be used to complement immunofluorescence for the diagnosis of rabies, enabling the detection of unexpected lyssaviruses during rabies surveillance.
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Estandarización de la obtención de amplificados del genoma del virus de la rabia para su uso en estudios de epidemiología molecular
Directory of Open Access Journals (Sweden)
Andrés Páez
2002-03-01
Full Text Available Con el fin de perfeccionar el diagnóstico y la tipificación del virus de la rabia en Colombia, el Laboratorio Nacional de Referencia del Instituto Nacional de Salud estandarizó técnicas para amplificar un fragmento de ADN complementario (ADNc a una fracción de 902 nucleótidos seleccionados del ARN del virus de la rabia. La fracción mencionada contiene secuencias que codifican para los aminoácidos 447-525 de la glicoproteína y 1-35 de la proteína L. Además, contiene la región intergénica no codificante conocida como Pseudogen Psi. Las técnicas estandarizadas consistieron en: 1 amplificación biológica mediante reaislamiento del virus en ratón ICR, 2 extracción del ARN total a partir del cerebro del ratón infectado, y 3 amplificación molecular utilizando la técnica de transcripción reversa y reacción en cadena de la polimerasa (RT-PCR para obtener el ADNc. La muestra del estudio constó de 30 cepas de virus de rabia obtenidas originalmente de 30 caninos y tomadas del banco del Laboratorio de Virología. Por su simplicidad, la metodología descrita en este estudio presenta grandes ventajas sobre las técnicas descritas en anteriores estudios. La tecnología propuesta es un complemento preciso de las técnicas de diagnóstico de la rabia; además, se aplica a la identificación de relaciones filogenéticas de diversos aislamientos y, por ende, se utiliza en la identificación de las dinámicas de transmisión y difusión geográfica del virus rábico.
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System of programming units for the K556RT4 and K556RT5 fixed programmed memory devices
International Nuclear Information System (INIS)
Bobkov, S.G.; Ermolin, Yu.V.; Kantserov, V.A.; Strigin, V.B.
1983-01-01
The programming system of constant programmable memory devices K556RT4 and K556RT5 that consist of two units (a programming device and an electrothermotraining unit) is described. The modules are made in the KAMAK standard. The programming device takes up 2 normal places, while the electrothermotraining block takes up 1 place. As information recording is done using a computer the time for programming is reduced and the possibility of errors is limited as compared with the manual method. The computer introduces the whole word to be recorded, not the separate parts, in the programming device. The transition to a new digit of a given word in the programming device is done automatically. This reduces the expense of computer time and accelerates the programming of microdiagrams
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Application and evaluation of RT-PCR-ELISA for the nucleoprotein and RT-PCR for detection of low-pathogenic H5 and H7 subtypes of avian influenza virus
DEFF Research Database (Denmark)
Dybkær, Karen; Munch, Mette; Handberg, Kurt J.
2004-01-01
Three 1-tube Reverse Transcriptase Polymerase Chain Reactions (RT-PCR) directed against the genes encoding the nucleoprotein (NP) and the H5 and H7 hemagglutinin (HA) gene, respectively, were used for detection of avian influenza virus (AIV) in various specimens. A total of 1,040 samples...... originating from chickens experimentally infected with 2 different low pathogenic avian influenza viruses, from domestic ducks and from wild aquatic birds were examined. The outcome of 1) the universal AIV RT-PCR including a PCR-enzyme-linked immunosorbent assay (ELISA) procedure directed against NP (NP RT...
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Rapport fra VIOL-projektet, Del 2: Technological literacy som udfordring for professioner og uddannelse
DEFF Research Database (Denmark)
Rapporten ”Technological literacy som udfordring for professioner og uddannelse” er del af den samlede afrapportering af projektet ”Velfærdsteknologi, Innovation, Omsorg og Læring” (VIOL). VIOL-projektet er gennemført i et samarbejde mellem University College Sjælland (UCSJ) og Roskilde Universitet...... (RUC) og er støttet af Den Europæiske Socialfond. Projektet er gennemført i perioden januar 2013 til og med februar 2015. Rapporten ”Technological literacy - analyser og erfaringsopsamling på tværs af professioner” samler og formidler en tværgående analyse af projektets erfaringer og resultater med...... særligt fokus på analyser og fortolkninger af ”technological literacy” på tværs af professioner og ud-dannelser....
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Standardized comparison of the relative impacts of HIV-1 reverse transcriptase (RT) mutations on nucleoside RT inhibitor susceptibility.
Science.gov (United States)
Melikian, George L; Rhee, Soo-Yon; Taylor, Jonathan; Fessel, W Jeffrey; Kaufman, David; Towner, William; Troia-Cancio, Paolo V; Zolopa, Andrew; Robbins, Gregory K; Kagan, Ron; Israelski, Dennis; Shafer, Robert W
2012-05-01
Determining the phenotypic impacts of reverse transcriptase (RT) mutations on individual nucleoside RT inhibitors (NRTIs) has remained a statistical challenge because clinical NRTI-resistant HIV-1 isolates usually contain multiple mutations, often in complex patterns, complicating the task of determining the relative contribution of each mutation to HIV drug resistance. Furthermore, the NRTIs have highly variable dynamic susceptibility ranges, making it difficult to determine the relative effect of an RT mutation on susceptibility to different NRTIs. In this study, we analyzed 1,273 genotyped HIV-1 isolates for which phenotypic results were obtained using the PhenoSense assay (Monogram, South San Francisco, CA). We used a parsimonious feature selection algorithm, LASSO, to assess the possible contributions of 177 mutations that occurred in 10 or more isolates in our data set. We then used least-squares regression to quantify the impact of each LASSO-selected mutation on each NRTI. Our study provides a comprehensive view of the most common NRTI resistance mutations. Because our results were standardized, the study provides the first analysis that quantifies the relative phenotypic effects of NRTI resistance mutations on each of the NRTIs. In addition, the study contains new findings on the relative impacts of thymidine analog mutations (TAMs) on susceptibility to abacavir and tenofovir; the impacts of several known but incompletely characterized mutations, including E40F, V75T, Y115F, and K219R; and a tentative role in reduced NRTI susceptibility for K64H, a novel NRTI resistance mutation.
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Kirjanduskriitikute suvemängud / Kärt Hellerma
Index Scriptorium Estoniae
Hellerma, Kärt, 1956-
2007-01-01
Arvustus: Lodge, David. Ühest kohast teise / tlk. Aet Varik. Tallinn : Varrak, 2005 ; Lodge, David. Väike maailm / tlk. Kersti Unt. Tallinn : Varrak, 1996 ; Lodge, David. Väärt töö / tlk. Kersti Unt. Tallinn : Varrak, 2006
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ESTRATEGIA PARA REDUCIR INTERVALOS DE INCERTIDUMBRE APLICADA EN LOCALIZACIÓN DE FALLAS EN SISTEMAS DE DISTRIBUCIÓN
Directory of Open Access Journals (Sweden)
César Augusto Orozco Henao
2012-01-01
Full Text Available El método de localización propuesto en este artículo se basa en una estrategia iterativa que determina el valor mínimo de la reactancia de falla, utilizando la técnica de reducción de intervalos de incertidumbre de Fibonacci. Esta técnica mejora el método de localización de fallas debido a que aumenta su precisión, mediante la aplicación de un tamaño de paso variable para determinar el valor absoluto de la reactancia de falla. La estrategia utiliza las mediciones de tensión y corriente en la subestación, por lo tanto, considera las variaciones en los estados de pre-falla y falla. El método se valida en el sistema de prueba IEEE 34 nodos conformado por laterales monofásicos, bifásicos y trifásicos, para todos los tipos de fallas y considerando resistencias de falla entre 0 y 40ohms. Los resultados obtenidos muestran errores de estimación inferiores al 1,8%, lo cual confirma la validez de la propuesta.
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Modeling qRT-PCR dynamics with application to cancer biomarker quantification.
Science.gov (United States)
Chervoneva, Inna; Freydin, Boris; Hyslop, Terry; Waldman, Scott A
2017-01-01
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is widely used for molecular diagnostics and evaluating prognosis in cancer. The utility of mRNA expression biomarkers relies heavily on the accuracy and precision of quantification, which is still challenging for low abundance transcripts. The critical step for quantification is accurate estimation of efficiency needed for computing a relative qRT-PCR expression. We propose a new approach to estimating qRT-PCR efficiency based on modeling dynamics of polymerase chain reaction amplification. In contrast, only models for fluorescence intensity as a function of polymerase chain reaction cycle have been used so far for quantification. The dynamics of qRT-PCR efficiency is modeled using an ordinary differential equation model, and the fitted ordinary differential equation model is used to obtain effective polymerase chain reaction efficiency estimates needed for efficiency-adjusted quantification. The proposed new qRT-PCR efficiency estimates were used to quantify GUCY2C (Guanylate Cyclase 2C) mRNA expression in the blood of colorectal cancer patients. Time to recurrence and GUCY2C expression ratios were analyzed in a joint model for survival and longitudinal outcomes. The joint model with GUCY2C quantified using the proposed polymerase chain reaction efficiency estimates provided clinically meaningful results for association between time to recurrence and longitudinal trends in GUCY2C expression.
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Reverse transcriptase-quantitative polymerase chain reaction (RT ...
African Journals Online (AJOL)
zino
2014-02-05
Feb 5, 2014 ... ecological studies - A review ... The objective of this review is to assess the importance of RT-qPCR in soil related ... phenol extraction step with heat inactivation of the added .... Real time polymerase chain reaction (PCR).
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Situación psicosocial de las adolescentes y toxemias del embarazo
Directory of Open Access Journals (Sweden)
Salvador-Moysén Jaime
2000-01-01
Full Text Available OBJETIVO: Conocer la situación psicosocial percibida durante la gestación por dos grupos de adolescentes; uno de ellos, conformado por mujeres con diagnóstico de enfermedad hipertensiva inducida por el embarazo y, el otro, constituido por mujeres cualificadas como "sanas". MATERIAL Y MÉTODOS: Se eligió un diseño de casos y controles. De las adolescentes gestantes que acudieron para la resolución de su embarazo al Hospital General de la ciudad de Durango, Durango, en los periodos de julio de 1996 a febrero de 1997, y de septiembre de 1998 a enero de 1999, se seleccionaron 39 casos y 88 controles de acuerdo con criterios explícitos del estudio, a quienes se les aplicó un cuestionario semiestructurado para la identificación de: estresores psicosociales, apoyo psicosocial, respuestas de enfrentamiento, intencionalidad, deseo y aceptación del embarazo, estado anímico y calidad de convivencia durante el mismo. Para el análisis de los datos, además de los procedimientos descriptivos, se estimó ji² y la razón de momios con intervalos de confianza a 95%. RESULTADOS: Las características sociodemográficas y ginecobstétricas de ambos grupos fueron similares; la percepción de fuentes de estrés fue mayor en el grupo de casos y la de fuentes de apoyo favoreció al grupo control, el estado anímico positivo y la convivencia satisfactoria durante el embarazo fueron referidos más frecuentemente con valores estadísticamente significativos, por las integrantes del grupo control. CONCLUSIONES: Se identificaron diferencias cualitativas en la percepción de los escenarios psicosociales por las mujeres participantes en el estudio, observándose una evaluación más favorable, estadísticamente significativa, por el grupo control.
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Pärt, Arvo. Litany; Psalom; Trisagion / Andreas Obst
Index Scriptorium Estoniae
Obst, Andreas
1996-01-01
Pärt, Arvo. Litany; Psalom; Trisagion. Hilliard Ensemble, Estonian Philharmonic Chamber Choir, Tallinn Chamber Orchestra, Tõnu Kaljuste; Lithuanian Chamber Orchestra, Saulius Sondeckis. ECM 1592/449 810-2
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Narcisisma saistība ar pašnovērtējumu.
OpenAIRE
Strode, Indra
2008-01-01
Pētījuma mēķis ir noskaidrot narcisisma saistību ar pašvērtējumu. Pētījuma respondentu skaits – 60 cilvēki (30 sievietes, 30vīrieši). Vecuma ierobežojums – no 20 līdz 30 gadi. Pašvērtējuma līmeņa noteikšanai tika izmantota Rozenberga pašnovērtējuma aptauja („Rosenberg Self-Esteem Scale”, Rosenberg, 1965), narcisisma noteikšanai tik izmantota Narcistiskas personības aptauja (Raskin & Terry, 1988). Vīriešu narcisisma un pašvērtējuma rādītāji uzrādīja statistiski nozīmīgu korelāciju, tāpat kā si...
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Evaluación de conformidad en la calibración del canal de medición de conductividad para aguas farmacéuticas
Directory of Open Access Journals (Sweden)
Adolfo Castillo Caballero
2017-12-01
Full Text Available Entre los sistemas críticos para la producción de productos inyectables en el Centro de Inmunología Molecular se encuentra el canal de medición de la conductividad, tanto para agua purificada como para el agua para inyección. De acuerdo con el cumplimiento de las Buenas Prácticas de Producción, estos sistemas se someten regularmente a procesos de validación, donde la calibración del canal de conductividad es una práctica obligatoria para la calificación del desempeño. Un equipo o sistema de medición para ser fiable requiere una evaluación de la conformidad, con una probabilidad mayor o igual al 95%. Para dar cumplimiento a los requisitos regulatorios y directrices actuales se propone como criterio la evaluación de la conformidad en la calibración del sistema de agua farmacéutica. Se armoniza el procedimiento de calibración y las regulaciones farmacéuticas internacionales actuales para la medición de la conductividad del agua para productos inyectables. Se definieron las características metrológicas, las fuentes de incertidumbre se cuantifican y se determinan los intervalos de aceptación, para conseguir una probabilidad de la conformidad en la calibración igual al 95% a partir del conocimiento de la capacidad de medición, con el fin de responder a los requerimientos de las agencias regulatorias internacionales para la industria biofarmacéutica.
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Mamma diagnostics for MTRA (medical-radiological personnel)/RT (radiologists); Mammadiagnostik fuer MTRA/RT
Energy Technology Data Exchange (ETDEWEB)
Fischer, Uwe; Baum, Friedemann
2014-07-01
The text book on mamma diagnostics for MTRA (medical-radiological personnel)/RT (radiologists) covers the following issues: Anatomy, development and physiology of mammary glands; tumor development an breast cancer risk; pathology, non-imaging diagnostics; mammography: physical-technical fundamentals; mammography: analogue technique; mammography: digital technique; mammography: quality assurance; mammography: legal questions and radiation protection; mammography: new developments; mammography: setting technique; mammography: use and appraisal; mamma-sonography: technique and methodology; mamma-sonography: assignment and appraisal, mamma-NMR: technique and methodology; mamma-NMR: assignment and appraisal lymph node diagnostics; mamma interventions; biopsy; mamma interventions: marking examination concepts; therapeutic concepts; hygienic concepts; communication and interaction.
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Canine distemper virus detection by different methods of One-Step RT-qPCR
Directory of Open Access Journals (Sweden)
Claudia de Camargo Tozato
2016-01-01
Full Text Available ABSTRACT: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR assays were tested and compared for analytical sensitivity to Canine Distemper Virus RNA detection: a One-Step RT-qPCR (system A and a One-Step RT-qPCR combined with NESTED-qPCR (system B. Limits of detection for both systems were determined using a serial dilution of Canine Distemper Virus synthetic RNA or a positive urine sample. In addition, the same urine sample was tested using samples with prior centrifugation or ultracentrifugation. Commercial kits of One-Step RT-qPCR assays detected canine distemper virus RNA in 10 (100% urine samples from symptomatic animals tested. The One-Step RT-qPCR kit that showed better results was used to evaluate the analytical sensitivity of the A and B systems. Limit of detection using synthetic RNA for the system A was 11 RNA copies µL-1 and 110 RNA copies µl-1 for first round System B. The second round of the NESTED-qPCR for System B had a limit of detection of 11 copies µl-1. Relationship between Ct values and RNA concentration was linear. The RNA extracted from the urine dilutions was detected in dilutions of 10-3 and10-2 by System A and B respectively. Urine centrifugation increased the analytical sensitivity of the test and proved to be useful for routine diagnostics. The One-Step RT-qPCR is a fast, sensitive and specific method for canine distemper routine diagnosis and research projects that require sensitive and quantitative methodology.
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Pärt, Arvo: "Miserere", "Festina lente" / Volkmar Fischer
Index Scriptorium Estoniae
Fischer, Volkmar
1991-01-01
Uuest heliplaadist Pärt, Arvo: "Miserere", "Festina lente", "Sarah Was Ninety Years Old". The Hilliard Ensemble. Orchester der Beethovenhalle Bonn. Dirigent Dennis Russel Davies (ECM Records, distr Amigo)
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MANIFESTACIONES DE VIOLENCIA EXPLÍCITA O EVIDENTE DURANTE EL DESARROLLO DEL RECREO ESCOLAR
Directory of Open Access Journals (Sweden)
Jenny María Artavia Granados
2012-01-01
Full Text Available Este artículo es producto de un proceso de investigación realizado en una escuela de la Dirección Regional de Educación Occidente. Su finalidad fue determinar si en el intervalo de los recreos de la escuela en estudio, se suscitaban manifestaciones de violencia escolar explícita entre estudiantes de IV, V y VI año del II Ciclo de la Educación General Básica. Durante el desarrollo del estudio, se tomaron en cuenta aspectos relacionados con las actividades lúdicas y las interacciones personales que se presentaban en estos espacios escolares. Sobre todo, los que podrían propiciar violencia escolar explícita; los mecanismos de prevención y estrategias empleadas por la escuela para contrarrestar la violencia escolar; también el nivel de integración del personal docente y administrativo de la escuela durante el periodo de los recreos. Asimismo, se plantearon algunas conclusiones entre las que destacan que un alto porcentaje de niños y niñas manifiesta que existen enfrentamientos entre estudiantes, durante el desarrollo de los recreos, y que estos se perciben a través de comportamientos de violencia tanto física como verbal. Las manifestaciones de violencia más comunes son peleas, golpes así como el uso de un lenguaje soez y ofensivo. Los motivos de los enfrentamientos varían según el nivel escolar entre el estudiantado. Los mecanismos empleados por la escuela para contrarrestar la violencia que se suscita durante los recreos, se basan en el uso de procedimientos sancionadores y la aplicación de medidas preventivas.
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Detection of Tomato black ring virus by real-time one-step RT-PCR.
Science.gov (United States)
Harper, Scott J; Delmiglio, Catia; Ward, Lisa I; Clover, Gerard R G
2011-01-01
A TaqMan-based real-time one-step RT-PCR assay was developed for the rapid detection of Tomato black ring virus (TBRV), a significant plant pathogen which infects a wide range of economically important crops. Primers and a probe were designed against existing genomic sequences to amplify a 72 bp fragment from RNA-2. The assay amplified all isolates of TBRV tested, but no amplification was observed from the RNA of other nepovirus species or healthy host plants. The detection limit of the assay was estimated to be around nine copies of the TBRV target region in total RNA. A comparison with conventional RT-PCR and ELISA, indicated that ELISA, the current standard test method, lacked specificity and reacted to all nepovirus species tested, while conventional RT-PCR was approximately ten-fold less sensitive than the real-time RT-PCR assay. Finally, the real-time RT-PCR assay was tested using five different RT-PCR reagent kits and was found to be robust and reliable, with no significant differences in sensitivity being found. The development of this rapid assay should aid in quarantine and post-border surveys for regulatory agencies. Copyright © 2010 Elsevier B.V. All rights reserved.
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Análisis espacial del sobrepeso y la obesidad infantil en el Perú, 2014
Directory of Open Access Journals (Sweden)
Akram Hernández-Vásquez
Full Text Available RESUMEN Objetivos. Estimar las prevalencias regionales e identificar los patrones espaciales del grado de sobrepeso y obesidad por distritos, en menores de cinco años en el Perú durante el año 2014. Materiales y métodos. Análisis de la información reportada por el Sistema de Información del Estado Nutricional (SIEN del número de casos de sobrepeso y obesidad en menores de cinco años registrados durante el año 2014. Se calcularon prevalencias regionales para sobrepeso y obesidad y sus respectivos intervalos de confianza al 95%. El índice de Moran fue utilizado para determinar los patrones de agrupación de distritos con altas prevalencia de sobrepeso y/o obesidad. Resultados. Se analizaron datos de 1834 distritos y 2 318 980 menores de cinco años, 158 738 casos (6,84%; IC 95%: 6,81-6,87 presentaron sobrepeso, mientras que 56 125 (2,42%; IC 95%: 2,40-2,44 obesidad. Las mayores prevalencias de sobrepeso fueron identificadas en las regiones de Tacna (13,9%, Moquegua (11,8%, Callao (10,4%, Lima (10,2% e Ica (9,3%, y en las mismas regiones para obesidad con 5,3%; 4,3%; 4,0%; 4,0% y 3,8% respectivamente. El análisis espacial encontró agrupaciones distritales de altas prevalencias en un 10% del total de distritos tanto para sobrepeso y obesidad, identificándose 199 distritos para sobrepeso (126 urbanos y 73 rurales, y 184 para obesidad (136 urbanos y 48 rurales. Conclusiones. Se identificó las mayores prevalencias de sobrepeso y obesidad en regiones de la costa peruana. Asimismo, estas regiones son las que exhibieron predominantemente una agrupación espacial de distritos con altas prevalencias de sobrepeso y obesidad.
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Monitoring and improving the sensitivity of dengue nested RT-PCR used in longitudinal surveillance in Thailand.
Science.gov (United States)
Klungthong, Chonticha; Manasatienkij, Wudtichai; Phonpakobsin, Thipwipha; Chinnawirotpisan, Piyawan; Rodpradit, Prinyada; Hussem, Kittinun; Thaisomboonsuk, Butsaya; Ong-ajchaowlerd, Prapapun; Nisalak, Ananda; Kalayanarooj, Siripen; Buddhari, Darunee; Gibbons, Robert V; Jarman, Richard G; Yoon, In-Kyu; Fernandez, Stefan
2015-02-01
AFRIMS longitudinal dengue surveillance in Thailand depends on the nested RT-PCR and the dengue IgM/IgG ELISA. To examine and improve the sensitivity of the nested RT-PCR using a panel of archived samples collected during dengue surveillance. A retrospective analysis of 16,454 dengue IgM/IgG ELISA positive cases collected between 2000 and 2013 was done to investigate the sensitivity of the nested RT-PCR. From these cases, 318 acute serum specimens or extracted RNA, previously found to be negative by the nested RT-PCR, were tested using TaqMan real-time RT-PCR (TaqMan rRT-PCR). To improve the sensitivity of nested RT-PCR, we designed a new primer based on nucleotide sequences from contemporary strains found to be positive by the TaqMan rRT-PCR. Sensitivity of the new nested PCR was calculated using a panel of 87 samples collected during 2011-2013. The percentage of dengue IgM/IgG ELISA positive cases that were negative by the nested RT-PCR varied from 17% to 42% for all serotypes depending on the year. Using TaqMan rRT-PCR, dengue RNA was detected in 194 (61%) of the 318 acute sera or extracted RNA previously found to be negative by the nested RT-PCR. The newly designed DENV-1 specific primer increased the sensitivity of DENV-1 detection by the nested RT-PCR from 48% to 88%, and of all 4 serotypes from 73% to 87%. These findings demonstrate the impact of genetic diversity and signal erosion on the sensitivity of PCR-based methods. Published by Elsevier B.V.
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Pärt, Arvo: Seven Magnificat Antiphons / Barry Witherden
Index Scriptorium Estoniae
Witherden, Barry
1996-01-01
Uuest heliplaadist "Pärt, Arvo: Seven Magnificat Antiphons. Magnificat. Summa; Tormis, Veljo: The Curse Upon Iron. Karelian Destiny. BBC Singers, Bo Holton". Collins Classics 1472-2 (61 minutes: DDD)
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Comportamiento reológico dinámico de mayonesas comerciales: influencia de la temperatura y del contenido en aceite
Directory of Open Access Journals (Sweden)
Berjano, M.
1991-10-01
Full Text Available The linear dynamic viscoelasticity of two commercial foodstuffs, oil in water emulsions with very different oil contents, has been studied. Temperature ranged between 10 and 40°C. From the experimental results it can be deduced that less oil content produces a significant decrease in the linear viscoelastic functions, as well as an enhanced temperature sensitivity.
Se estudia el comportamiento viscoelástico dinámico, en zona lineal, de dos productos comerciales que son emulsiones alimentarias aceite en agua, o/w, con un contenido en aceite muy distinto. El intervalo de temperatura estudiado ha variado entre 10 y 40°C. De los resultados obtenidos se deduce que un menor contenido en aceite produce una disminución significativa de todas las funciones viscoelásticas, así como una mayor influencia del efecto de la temperatura.
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Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR
Directory of Open Access Journals (Sweden)
Sérgio Monteiro de Almeida
Full Text Available ABSTRACT The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR. The aim of this study was to examine the influence of red blood cells (RBCs in cerebrospinal fluid (CSF as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR for enteroviruses (EV. Forty-four CSF samples from patients showing characteristics of viral meningitis were assessed for EV by RT-PCR. Viral RNA extracted with guanidine isothyocianate buffer and virus detection was performed by in-house nested PCR. Positivity for EV RT-PCR was higher in CSF samples without RBCs than in samples with RBCs: 13(26% and 36(9.2%, p = 0.001. In the group with positive EV RT-PCR, the mean + SD CSF RBC was 37 ± 183 cell/mm3; the group with negative results had 580 + 2,890 cell/mm3 (p = 0.007. The acceptable upper limit for CSF RBCs that could not influence RT-PCR was 108 cells/mm3. CSF samples with negative results for EV RT-PCR have more erythrocytes.
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SPoRT - An End-to-End R2O Activity
Science.gov (United States)
Jedlovec, Gary J.
2009-01-01
Established in 2002 to demonstrate the weather and forecasting application of real-time EOS measurements, the Short-term Prediction Research and Transition (SPoRT) program has grown to be an end-to-end research to operations activity focused on the use of advanced NASA modeling and data assimilation approaches, nowcasting techniques, and unique high-resolution multispectral observational data applications from EOS satellites to improve short-term weather forecasts on a regional and local scale. SPoRT currently partners with several universities and other government agencies for access to real-time data and products, and works collaboratively with them and operational end users at 13 WFOs to develop and test the new products and capabilities in a "test-bed" mode. The test-bed simulates key aspects of the operational environment without putting constraints on the forecaster workload. Products and capabilities which show utility in the test-bed environment are then transitioned experimentally into the operational environment for further evaluation and assessment. SPoRT focuses on a suite of data and products from MODIS, AMSR-E, and AIRS on the NASA Terra and Aqua satellites, and total lightning measurements from ground-based networks. Some of the observations are assimilated into or used with various versions of the WRF model to provide supplemental forecast guidance to operational end users. SPoRT is enhancing partnerships with NOAA / NESDIS for new product development and data access to exploit the remote sensing capabilities of instruments on the NPOESS satellites to address short term weather forecasting problems. The VIIRS and CrIS instruments on the NPP and follow-on NPOESS satellites provide similar observing capabilities to the MODIS and AIRS instruments on Terra and Aqua. SPoRT will be transitioning existing and new capabilities into the AWIIPS II environment to continue the continuity of its activities.
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Tabaquismo en profesionales de la salud del Instituto Mexicano del Seguro Social, Morelos
Directory of Open Access Journals (Sweden)
Jorge Salmerón-Castro
2002-01-01
Full Text Available Objetivo. Determinar la prevalencia de hábito tabáquico por categoría laboral en trabajadores de la salud del Instituto Mexicano del Seguro Social (IMSS, en el estado de Morelos, México. Material y métodos. Se realizó un estudio transversal en 3 133 empleados de los servicios médicos de las 23 unidades médicas con que cuenta la delegación Morelos del IMSS, durante el periodo de octubre de 1998 a marzo de 2000. Mediante un cuestionario autoaplicable se recolectó información sobre patrones de consumo de tabaco en distintas etapas de la vida, así como algunas características demográficas y categoría laboral. Se estimaron prevalencias e intervalos de confianza al 95% por grupos de edad y sexo para distintas categorías laborales. Resultados. De los 3 133 empleados participantes, 53.4% (IC 95% 50.8-56.8 de los hombres y 27.4% (IC 95% 25.4-29.3 de las mujeres contaban con historia de tabaquismo en algún momento de la vida. La prevalencia de tabaquismo activo fue de 28.3 % (IC 95% 25.6-31.0 y 14.4% (IC 95% 12.8-15.9 en hombres y mujeres, respectivamente. Entre los hombres, los médicos presentan una prevalencia de tabaquismo activo menor (20.9% que la observada entre trabajadores de otras áreas: personal administrativo (33.0% y personal técnico de distintas categorías (26.6%. Las mujeres muestran en general una prevalencia menor que los hombres en todas las categorías, y las enfermeras presentan una prevalencia discretamente menor (12.5% que las médicas (16.0%. Conclusiones. El patrón del consumo de tabaco observado en profesionales de la salud del IMSS Morelos muestra una prevalencia menor a la observada en población general. Sin embargo, es necesario continuar con programas para desalentar el consumo del tabaco entre profesionales de la salud, y tratar de hacerlos concientes acerca de la influencia positiva que pueden y deben ejercer en la sociedad para impulsar programas integrados contra el consumo del tabaco y la adicción a
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Short-Term Prediction Research and Transition (SPoRT) Center: Transitioning Satellite Data to Operations
Science.gov (United States)
Zavodsky, Bradley
2012-01-01
The Short-term Prediction Research and Transition (SPoRT) Center located at NASA Marshall Space Flight Center has been conducting testbed activities aimed at transitioning satellite products to National Weather Service operational end users for the last 10 years. SPoRT is a NASA/NOAA funded project that has set the bar for transition of products to operational end users through a paradigm of understanding forecast challenges and forecaster needs, displaying products in end users decision support systems, actively assessing the operational impact of these products, and improving products based on forecaster feedback. Aiming for quality partnerships rather than a large quantity of data users, SPoRT has become a community leader in training operational forecasters on the use of up-and-coming satellite data through the use of legacy instruments and proxy data. Traditionally, SPoRT has supplied satellite imagery and products from NASA instruments such as the Moderate-resolution Imaging Spectroradiometer (MODIS) and the Atmospheric Infrared Sounder (AIRS). However, recently, SPoRT has been funded by the GOES-R and Joint Polar Satellite System (JPSS) Proving Grounds to accelerate the transition of selected imagery and products to help improve forecaster awareness of upcoming operational data from the Visible Infrared Imager Radiometer Suite (VIIRS), Cross-track Infrared Sounder (CrIS), Advanced Baseline Imager (ABI), and Geostationary Lightning Mapper (GLM). This presentation provides background on the SPoRT Center, the SPoRT paradigm, and some example products that SPoRT is excited to work with forecasters to evaluate.
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Märt Agu: "Isa vari käib mul kogu aeg kannul" / Märt Agu ; interv. Aigi Viira
Index Scriptorium Estoniae
Agu, Märt
2006-01-01
1. okt. tähistas oma 30-aastast sünnipäeva ja oma ellukutsuja Mait Agu 55-sünniaastapäeva Tallinna Ülikooli koreograafiaosakond. Sel puhul esitati Estonias Mait Agu tantsulavastus "Kodalased ja kaasaegsed", mille seadis uuesti lavale Märt Agu oma isa loodud koreograafia põhjal
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A Formal Approach for RT-DVS Algorithms Evaluation Based on Statistical Model Checking
Directory of Open Access Journals (Sweden)
Shengxin Dai
2015-01-01
Full Text Available Energy saving is a crucial concern in embedded real time systems. Many RT-DVS algorithms have been proposed to save energy while preserving deadline guarantees. This paper presents a novel approach to evaluate RT-DVS algorithms using statistical model checking. A scalable framework is proposed for RT-DVS algorithms evaluation, in which the relevant components are modeled as stochastic timed automata, and the evaluation metrics including utilization bound, energy efficiency, battery awareness, and temperature awareness are expressed as statistical queries. Evaluation of these metrics is performed by verifying the corresponding queries using UPPAAL-SMC and analyzing the statistical information provided by the tool. We demonstrate the applicability of our framework via a case study of five classical RT-DVS algorithms.
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Reactividad del antígeno GST-SAPA de Trypanosoma cruzi frente a sueros de pacientes con enfermedad de Chagas y leishmaniasis Reactivity of GST-SAPA antigen of Trypanosoma cruzi against sera from patients with Chagas disease and leishmaniasis
Directory of Open Access Journals (Sweden)
José Gil
2011-04-01
Full Text Available El diagnóstico serológico de la infección producida por Trypanosoma cruzi es de especial relevancia dado que los métodos parasitológicos tienen, en las fases indeterminada y crónica, una sensibilidad limitada. El antígeno SAPA fue usado en diversos estudios y demostró ser un buen candidato para el diagnóstico de la infección por T. cruzi. La enfermedad de Chagas y la leishmaniasis son endémicas en el norte de Salta, con posibles zonas de solapamiento. Este hecho suele dar lugar a infecciones mixtas T. cruzi-Leishmania spp., con la consecuente probabilidad de diagnóstico cruzado cuando se usan antígenos no específicos. Se evaluó la reactividad del antígeno GST-SAPA en la prueba de ELISA (ELISA-SAPA frente a sueros de personas infectadas por T. cruzi (n = 154, con leishmaniasis (n = 66, infecciones mixtas (n = 29 y controles negativos (n = 28, usando como pruebas de referencia para el diagnóstico de la infección por T. cruzi kits comerciales de ELISA y HAI. Se calculó la sensibilidad, especificidad e índice de concordancia kappa de la prueba de ELISA-SAPA, para la detección de infección por T. cruzi. Entre los sueros de pacientes con leishmaniasis estudiados se detectó un 30.5% de infecciones mixtas. Para la detección de infección por T. cruzi, ELISA-SAPA mostró una sensibilidad del 97.1% (intervalo de confianza del 95%: 94.5-99.9, una especificidad del 100% (intervalo de confianza del 95%: 99.5-100 y un índice de concordancia kappa de 96 (intervalo de confianza del 95%:93-99%, comparado con las pruebas serológicas comerciales. Los valores de sensibilidad, especificidad y concordancia calculados muestran una alta eficiencia de ELISA-SAPA.Serologic diagnosis of Trypanosoma cruzi infection is important due to the limited sensitivity of direct parasitologic methods for diagnosis in the indeterminate and chronic phases of disease. SAPA antigen has been used in several studies and has been shown to be a good marker for use
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Ring trial 2016 for Bluetongue virus detection by real-time RT-PCR in France.
Science.gov (United States)
Sailleau, Corinne; Viarouge, Cyril; Breard, Emmanuel; Vitour, Damien; Zientara, Stephan
2017-05-01
Since the unexpected emergence of BTV-8 in Northern Europe and the incursion of BTV-8 and 1 in France in 2006-2007, molecular diagnosis has considerably evolved. Several real-time RT-PCR (rtRT-PCR) methods have been developed and published, and are currently being used in many countries across Europe for BTV detection and typing. In France, the national reference laboratory (NRL) for orbiviruses develops and validates 'ready-to-use' kits with private companies for viral RNA detection. The regional laboratories network that was set up to deal with a heavy demand for analyses has used these available kits. From 2007, ring tests were organized to monitor the performance of the French laboratories. This study presents the results of 63 regional laboratories in the ring trial organized in 2016. Blood samples were sent to the laboratories. Participants were asked to use the rtRT-PCR methods in place in their laboratory, for detection of all BTV serotypes and specifically BTV-8. The French regional laboratories are able to detect and genotype BTV in affected animals. Despite the use of several methods (i.e. RNA extraction and different commercial rtRT-PCRs), the network is homogeneous. The ring trial demonstrated that the French regional veterinary laboratories have reliable and robust BTV diagnostic tools for BTV genome detection.
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Efectos de la restricción comportamental crónica sobre el aprendizaje de una tarea espacial en el laberinto circular de Barnes
Directory of Open Access Journals (Sweden)
Alejandro Múnera
2005-03-01
Full Text Available Estudios anteriores utilizando modelos de memoria espacial basados en la natación forzada o la deprivación alimentaria han mostrado que el estrés por inmovilización aplicado de forma crónica (21 días afecta el aprendizaje en términos de la velocidad para aprender la tarea y del número de errores cometidos. Igualmente, se ha observado que el estrés deteriora la ejecución cognitiva despúes de largos intervalos y la mejora a intervalos cortos (despuesta adaptativa.
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PpRT1: the first complete gypsy-like retrotransposon isolated in Pinus pinaster.
Science.gov (United States)
Rocheta, Margarida; Cordeiro, Jorge; Oliveira, M; Miguel, Célia
2007-02-01
We have isolated and characterized a complete retrotransposon sequence, named PpRT1, from the genome of Pinus pinaster. PpRT1 is 5,966 bp long and is closely related to IFG7 gypsy retrotransposon from Pinus radiata. The long terminal repeats (LTRs) have 333 bp each and show a 5.4% sequence divergence between them. In addition to the characteristic polypurine tract (PPT) and the primer binding site (PBS), PpRT1 carries internal regions with homology to retroviral genes gag and pol. The pol region contains sequence motifs related to the enzymes protease, reverse transcriptase, RNAseH and integrase in the same typical order known for Ty3/gypsy-like retrotransposons. PpRT1 was extended from an EST database sequence indicating that its transcription is occurring in pine tissues. Southern blot analyses indicate however, that PpRT1 is present in a unique or a low number of copies in the P. pinaster genome. The differences in nucleotide sequence found between PpRT1 and IFG7 may explain the strikingly different copy number in the two pine species genome. Based on the homologies observed when comparing LTR region among different gypsy elements we propose that the highly conserved LTR regions may be useful to amplify other retrotransposon sequences of the same or close retrotransposon family.
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Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated associated virus 3 variant groups I, II, III and VI
Directory of Open Access Journals (Sweden)
Bester Rachelle
2012-09-01
Full Text Available Abstract Background Grapevine leafroll-associated virus 3 (GLRaV-3 is the main contributing agent of leafroll disease worldwide. Four of the six GLRaV-3 variant groups known have been found in South Africa, but their individual contribution to leafroll disease is unknown. In order to study the pathogenesis of leafroll disease, a sensitive and accurate diagnostic assay is required that can detect different variant groups of GLRaV-3. Methods In this study, a one-step real-time RT-PCR, followed by high-resolution melting (HRM curve analysis for the simultaneous detection and identification of GLRaV-3 variants of groups I, II, III and VI, was developed. A melting point confidence interval for each variant group was calculated to include at least 90% of all melting points observed. A multiplex RT-PCR protocol was developed to these four variant groups in order to assess the efficacy of the real-time RT-PCR HRM assay. Results A universal primer set for GLRaV-3 targeting the heat shock protein 70 homologue (Hsp70h gene of GLRaV-3 was designed that is able to detect GLRaV-3 variant groups I, II, III and VI and differentiate between them with high-resolution melting curve analysis. The real-time RT-PCR HRM and the multiplex RT-PCR were optimized using 121 GLRaV-3 positive samples. Due to a considerable variation in melting profile observed within each GLRaV-3 group, a confidence interval of above 90% was calculated for each variant group, based on the range and distribution of melting points. The intervals of groups I and II could not be distinguished and a 95% joint confidence interval was calculated for simultaneous detection of group I and II variants. An additional primer pair targeting GLRaV-3 ORF1a was developed that can be used in a subsequent real-time RT-PCR HRM to differentiate between variants of groups I and II. Additionally, the multiplex RT-PCR successfully validated 94.64% of the infections detected with the real-time RT-PCR HRM
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La edad materna avanzada como elemento favorecedor de complicaciones obstétricas y del nacimiento
Directory of Open Access Journals (Sweden)
Jorge Manuel Balestena Sánchez
2015-10-01
Full Text Available Introducción: el aumento actual del embarazo en mujeres añosas provoca un riesgo superior para la mujer y su feto. Objetivo: evaluar la edad materna avanzada como factor de complicaciones obstétricas y del nacimiento. Material y método: se efectuó una investigación observacional, retrospectiva, transversal y analítica en el Hospital General Docente "Abel Santamaría Cuadrado" de Pinar del Río entre 2012 y 2013. El universo de estudio fueron la totalidad de gestantes que ingresaron en el período estudiado. La muestra se formó con gestantes añosas (grupo estudio 360 pacientes y un grupo control (720 pacientes con embarazadas entre 25 y 30 años; ambos se escogieron por muestreo intencionado. Se resumieron las variables cualitativas mediante frecuencias absolutas y relativas porcentuales. Se utilizaron las pruebas de ji cuadrado, Odd Ratio. El intervalo de confianza para el Odd Ratio al 95%. Resultados: hubo un predominio de la embarazada con partos anteriores (92.8%, la normopeso (68.9%, el 34.4% padecían alguna enfermedad crónica, siendo la principal la hipertensión arterial; la anemia durante el embarazo estuvo presente en el 34.4%, el 88.3% parieron a término, de modo espontáneo; además preponderó la cesárea con un (52.2%. La morbilidad intraparto se manifestó en el 13.1%, mientras la puerperal en el 26.7%. Hubo una elevada asociación del embarazo prolongado con la enfermedad hipertensiva, el oligoamnios y la anemia (p < 0,001, también con la inducción del parto (p < 0,001 y la cesárea (p < 0,001, así como con la morbilidad puerperal (p < 0,001. Conclusiones: la edad materna avanzada es un factor de riesgo a tener en cuenta durante el proceso de la gestación y el nacimiento.
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Pérdida del precondicionamiento isquémico: un fenómeno relacionado con el envejecimiento
Directory of Open Access Journals (Sweden)
Jorge A. Belardi
2007-01-01
Full Text Available IntroducciónLa edad es un predictor independiente de riesgo en pacientes con enfermedad coronaria.Esto podría explicarse por la falta de adaptación a la isquemia miocárdica aguda. Elprecondicionamiento es un mecanismo por el cual episodios repetitivos de isquemia inducenen el miocardio una tolerancia mayor a episodios subsiguientes.ObjetivoEvaluar el desarrollo de precondicionamiento isquémico en pacientes añosos.Material y métodosSe incluyeron 65 pacientes sometidos a angioplastia coronaria electiva (< 70 años [n = 47] y≥ 70 años [n = 18]. Se evaluó el desarrollo de precondicionamiento durante tres períodos deoclusión coronaria. Por ECG intracoronario se midió la elevación del ST al final de cada dilatacióny se registró el porcentaje de resolución del ST a la tercera dilatación respecto del máximovalor registrado. Los datos se presentan como mediana e intervalo intercuartil 25/75%.ResultadosNo hubo diferencias significativas en las características clínicas basales. El máximo ST registrado,el ST a la tercera dilatación y el porcentaje de resolución del ST fueron 14 (9/24mm, 8 (4/14 mm y 23,8% (0/55,5 para los pacientes jóvenes y 9,5 (5/18 mm (p = ns, 6,5 (4/16 mm (p = ns y 5,5% (0/20 (p = 0,04 para los añosos. Al estratificar por grupos etarios,la proporción de pacientes que alcanzaron una resolución del ST ≥ 50% mostró una distribuciónlineal por chi cuadrado de tendencia (p = 0,025.ConclusionesNuestro estudio sugiere que el precondicionamiento isquémico se encontraría disminuidoen pacientes añosos. La funcionalidad de este mecanismo menguaría en forma progresivacon el envejecimiento.
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Frecuentación del servicio de urgencias y factores sociodemográficos
Directory of Open Access Journals (Sweden)
Braun T.
2002-01-01
Full Text Available Objetivos: La utilización por parte de la población de los recursos sanitarios se encuentra relacionada con los niveles de salud y con factores socioeconómicos. Los grupos de población con menor nivel socioeconómico tienen una mayor utilización de determinados recursos sanitarios; los servicios de urgencia podrían presentar un uso diferencial entre los distintos grupos de población. El presente estudio pretende identificar diferencias en el uso de los recursos de atención urgente entre grupos con diferentes características socioeconómicas. Material y métodos: Se realiza un estudio poblacional, en el que mediante el agrupamiento de las secciones censales de la ciudad de Santander, según variables sociodemográficas (edad, nivel de formación y nivel de empleo, se establecen áreas de la ciudad con similares características, mediante análisis de clusters. Se identifica el origen de los pacientes que consultan en el Servicio de Urgencias Hospitalarias (SUH del Hospital Marqués de Valdecilla, y se calcula la tasa de demanda para cada una de las áreas sociodemográficas. Resultados: El análisis de clusters es capaz de identificar cuatro agrupaciones de diferentes características socioeconómicas que se ajustan a barrios de la ciudad de Santander. Se aprecia una mayor utilización del SUH en los grupos sociales más desfavorecidos y de mayor edad media (odds ratio [OR] = 1,91; intervalo de confianza [IC] del 95% 1,73-2,1 de la agrupación 1 con respecto a la 4. La proporción de ingresos es superior en las agrupaciones más favorecidas, mientras que el número de usuarios que realizan visitas repetidas proceden de agrupaciones con menor nivel socioeconómico. Conclusión: La utilización del SUH está condicionada por las características sociodemográficas del área de residencia, identificándose mayor uso entre los grupos de más bajo nivel socioeconómico.
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NASA SPoRT JPSS PG Activities in Alaska
Science.gov (United States)
Berndt, Emily; Molthan, Andrew; Fuell, Kevin; McGrath, Kevin; Smith, Matt; LaFontaine, Frank; Leroy, Anita; White, Kris
2018-01-01
SPoRT (NASA's Short-term Prediction Research and Transition Center) has collaboratively worked with Alaska WFOs (Weather Forecast Offices) to introduce RGB (Red/Green/Blue false color image) imagery to prepare for NOAA-20 (National Oceanic and Atmospheric Administration, JPSS (Joint Polar Satellite System) series-20 satellite) VIIRS (Visible Infrared Imaging Radiometer Suite) and improve forecasting aviation-related hazards. Last R2O/O2R (Research-to-Operations/Operations-to-Research) steps include incorporating NOAA-20 VIIRS in RGB suite and fully transitioning client-side RGB processing to GINA (Geographic Information Network of Alaska) and Alaska Region. Alaska Region WFOs have been part of the successful R2O/O2R story to assess the use of NESDIS (National Environmental Satellite, Data, and Information Service) Snowfall Rate product in operations. SPoRT introduced passive microwave rain rate and IMERG (Integrated Multi-satellitE Retrievals for GPM (Global Precipitation Measurement)) (IMERG) to Alaska WFOs for use in radar-void areas and assessing flooding potential. SPoRT has been part of the multi-organization collaborative effort to introduce Gridded NUCAPS (NOAA Unique CrIS/ATMS (Crosstrack Infrared Sounder/Advanced Technology Microwave Sounder) Processing System) to the Anchorage CWSU (Center Weather Service Unit) to assess Cold Air Aloft events, [and as part of NOAA's PG (Product Generation) effort].
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Produtividade da biomassa de melissa em função de intervalo de cortes e doses de nitrogênio Biomass production of Lemon balm as a result of cutting interval and nitrogen levels
Directory of Open Access Journals (Sweden)
André May
2008-09-01
Full Text Available A espécie Melissa officinalis tem crescido em importância econômica no setor produtivo de plantas medicinais, contudo o nível de informações agronômicas a cerca da espécie é limitado. Dessa forma, foi instalado um experimento visando avaliar a influência do intervalo de cortes e de doses de nitrogênio sobre a produção de biomassa dessa espécie. O delineamento experimental utilizado foi o de blocos ao acaso, com três repetições, em esquema fatorial 4 x 4, sendo os fatores estudados o intervalo de cortes (30; 42; 54 e 66 dias e dose de nitrogênio (0; 60; 120 e 180 kg ha-1 de N. Foram avaliadas a altura da planta e massa seca da parte aérea. Observou-se maior altura da planta para maior intervalo entre corte. Maiores rendimentos de massa seca foram observados com a aplicação de 180 kg ha-1 de N, havendo uma resposta linear para a produção de massa seca com a aplicação de N.Cultivation of Melissa officinalis is increasing in economic importance in the market of medicinal plants, although the level of agronomic information concerning the specie is limited. To achieve those objectives, a trial was carried out to study the influence of cutting intervals and nitrogen levels in biomass production. The experimental design was in blocks with three replications in a 4 x 4 factorial cheme: cutting intervals (30; 42; 54 and 66 days and nitrogen (0; 60; 120 and 180 kg ha-1 of N. The characteristics of plant height and aerial part and accumulated dry mater were evaluated. Increasing the cutting intervals resulted in higher plants. The highest dry matter value was observed using 180 kg ha-1 of N, having a linear reply for the production of dry mass with the application of N.
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Utilidad del modelo de Jouyban-Acree para la estimación de la solubilidad del ibuprofeno y el naproxeno en algunas mezclas cosolventes
Directory of Open Access Journals (Sweden)
Edgar F. Vargas E.
2008-05-01
Full Text Available La predicción de la solubilidad de los fármacos en sistemas cosolventes de uso farmacéutico ha sido una sólida lí-nea de investigación a nivel académico e industrial desde hace varias décadas. Por esta razón, en la presente inves-tigación se evaluó la validez de una adaptación puntual del modelo de Jouyban-Acree (J-A en comparación con la utilidad de la ecuación de solubilidad logarítmica-lineal propuesta por Yalkowsky y Roseman (Y-R, para la estima-ción de la solubilidad del ibuprofeno (IBP y el naproxeno (NAP en mezclas cosolventes propilenoglicol + agua, en función de la composición cosolvente y de la temperatura, en el intervalo entre 20,0 y 40,0 °C. Los modelos de J-A y Y-R requieren únicamente de los valores experimentales de la solubilidad en equilibrio de los fármacos en los sol-ventes puros a las diferentes temperaturas de intéres. Desafiando las ecuaciones frente a valores experimentales presentados en la literatura se encontró que los valores obtenidos con los dos modelos semiempíricos son bastante similares entre sí y que presentan algunas desviaciones notorias respecto a los valores experimentales reportados en la literatura, especialmente en la estimación de la solubilidad del IBP. Estos resultados demuestran la necesidad de mejorar las estrategias teóricas disponibles para la estimación de esta propiedad, y así mismo, demuestran la im-portancia de la determinación experimental de la solubilidad de los fármacos, en función de la temperatura, en to-das aquellas mezclas cosolventes que pueden ser útiles durante el proceso de diseño de formulaciones líquidas ho-mogéneas a nivel industrial.
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ANÁLISIS DE SENSIBILIDAD ESPACIAL DE UN MODELO HIDRODINAMICO DEL EMBALSE PUNCHINÁ (ANTIOQUIA ANÁLISE DE SENSIBILIDADE ESPACIAL DE UM MODELO HIDRODINÂMICO DO RESERVATÓRIO PUNCHINÁ (ANTIOQUIA SPATIAL SENSITIVITY ANALYSIS OF A HYDRODYNAMIC MODEL OF THE PUNCHINÁ RESERVOIR (ANTIOQUIA
Directory of Open Access Journals (Sweden)
Carlos Alejandro Escobar
2010-12-01
Full Text Available Se presenta en este artículo una metodología que permite cuantificar explícitamente el valor óptimo de los parámetros numéricos de un modelo hidrodinámico, tales como la resolución de la malla (en dirección horizontal y vertical y el intervalo temporal de cálculo. El procedimiento se basa en un análisis de beneficio-costo, donde se busca obtener un balance entre la calidad de la solución numérica y el costo computacional requerido para lograrla. El carácter espacial del análisis de sensibilidad propuesto permite determinar en cada una de las celdas de la malla de cálculo la alteración en la solución numérica debida a variaciones en los parámetros físicos o numéricos que constituyen el modelo. Lo anterior posibilitó identificar en el interior del embalse Punchiná dónde y en qué grado son relevantes la resolución de la malla, el intervalo de cálculo temporal, la rugosidad del lecho y el coeficiente de viscosidad de remolino.Apresenta-se neste artigo uma metodologia que permite quantificar explicitamente o valor ótimo dos parâmetros numéricos de um modelo hidrodinâmico, tais como a resolução da malha (em direção horizontal e vertical e o intervalo temporal de cálculo. O procedimento baseia-se em uma análise de benefício-custo, onde se procura obter um balanço entre a qualidade da solução numérica e o custo computacional requerido para consegui-la. O caráter espacial da análise de sensibilidade proposta permite determinar em cada uma das células da malha de cálculo a alteração na solução numérica devida a variações nos parâmetros físicos ou numéricos que constituem o modelo. O anterior possibilitou identificar no interior do reservatório Punchiná onde e em que grau são relevantes a resolução da malha, o intervalo de cálculo temporário, a rugosidade do leito e o coeficiente de viscosidade de redemoinho.This paper presents a methodology to assess explicitly the optimal value of the
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Structural Evolution of the R-T Phase Boundary in KNN-Based Ceramics
KAUST Repository
Lv, Xiang
2017-10-04
Although a rhombohedral-tetragonal (R-T) phase boundary is known to substantially enhance the piezoelectric properties of potassium-sodium niobate ceramics, the structural evolution of the R-T phase boundary itself is still unclear. In this work, the structural evolution of R-T phase boundary from -150 °C to 200 °C is investigated in (0.99-x)K0.5Na0.5Nb1-ySbyO3-0.01CaSnO3-xBi0.5K0.5HfO3 (where x=0~0.05 with y=0.035, and y=0~0.07 with x=0.03) ceramics. Through temperature-dependent powder X-ray diffraction (XRD) patterns and Raman spectra, the structural evolution was determined to be Rhombohedral (R, <-125 °C) → Rhombohedral+Orthorhombic (R+O, -125 °C to 0 °C) → Rhombohedral+Tetragonal (R+T, 0 °C to 150 °C) → dominating Tetragonal (T, 200 °C to Curie temperature (TC)) → Cubic (C, >TC). In addition, the enhanced electrical properties (e.g., a direct piezoelectric coefficient (d33) of ~450±5 pC/N, a conversion piezoelectric coefficient (d33*) of ~580±5 pm/V, an electromechanical coupling factor (kp) of ~0.50±0.02, and TC~250 °C), fatigue-free behavior, and good thermal stability were exhibited by the ceramics possessing the R-T phase boundary. This work improves understanding of the physical mechanism behind the R-T phase boundary in KNN-based ceramics and is an important step towards their adoption in practical applications. This article is protected by copyright. All rights reserved.
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Narr kui ajastu metafoor / Kärt Hellerma
Index Scriptorium Estoniae
Hellerma, Kärt, 1956-
2001-01-01
William Shakespeare'i "Kuningas Lear" (lav. Priit Pedajas) ja Pedro Calder̤n de la Barca "Elu on unenägu" (lav. Ingo Normet) Eesti Draamateatris. Ilmunud ka kogumikus : Hellerma, Kärt. Avanenud ruum. Tallinn : Eesti Keele Sihtasutus, 2006, lk. 213-216. Pealk. Narr kui meie aja kangelane
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Pärt, Arvo: De profundis / Barry Witherden
Index Scriptorium Estoniae
Witherden, Barry
1997-01-01
Uuest heliplaadist "Pärt, Arvo: De profundis. Missa Sillabica. Solfeggio. And one of the Pharisees. Cantate Domino. Summa. Seven Magnificat Antiphons. The Beautitudes. Magnificat. Christopher Bowers-Broadbent (org.), Daniel Kennedy (perc.). Theatre of Voices / Paul Hillier (bass)". Harmonia Mundi HMU40 7182; HMU90 7182 (76 minutes:DDD)
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Cell-type-specific responses of RT4 neural cell lines to dibutyryl-cAMP: branch determination versus maturation
International Nuclear Information System (INIS)
Droms, K.; Sueoka, N.
1987-01-01
This report describes the induction of cell-type-specific maturation, by dibutyryl-cAMP and testololactone, of neuronal and glial properties in a family of cell lines derived from a rat peripheral neurotumor, RT4. This maturation allows further understanding of the process of determination because of the close lineage relationship between the cell types of the RT4 family. The RT4 family is characterized by the spontaneous conversion of one of the cell types, RT4-AC (stem-cell type), to any of three derivative cell types, RT4-B, RT4-D, or RT4-E, with a frequency of about 10(-5). The RT4-AC cells express some properties characteristic of both neuronal and glial cells. Of these neural properties expressed by RT4-AC cells, only the neuronal properties are expressed by the RT4-B and RT4-E cells, and only the glial properties are expressed by the RT4-D cells. This in vitro cell-type conversion of RT4-AC to three derivative cell types is a branch point for the coordinate regulation of several properties and seems to resemble determination in vivo. In our standard culture conditions, several other neuronal and glial properties are not expressed by these cell types. However, addition of dibutyryl-cAMP induces expression of additional properties, in a cell-type-specific manner: formation of long cellular processes in the RT4-B8 and RT4-E5 cell lines and expression of high-affinity uptake of gamma-aminobutyric acid, by a glial-cell-specific mechanism, in the RT4-D6-2 cell line. These new properties are maximally expressed 2-3 days after addition of dibutyryl-cAMP
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Medicamentos que podem induzir prolongamento do intervalo QT utilizados por idosos em domicílio
Directory of Open Access Journals (Sweden)
Josiane Macêdo Martins
2015-10-01
Full Text Available O intervalo QT (iQT, parâmetro eletrocardiográfico, é um biomarcador não invasivo da repolarização ventricular. O aumento do iQT é uma alteração que pode ser de considerável importância clínica, pois predispõe a torsade de pointes e morte cardíaca súbita. O objetivo do presente trabalho é identificar os medicamentos utilizados em domicílio por idosos, que podem induzir o prolongamento do iQT. Trata-se de um estudo quantitativo descritivo exploratório e retrospectivo, realizado em um hospital público de ensino. Foram incluídos 190 idosos com informação sobre uso domiciliar de medicamentos registrada em prontuário. A mediana da idade foi de 69,5 anos, sendo 99 (52,1% mulheres. O número de medicamentos utilizados por paciente em domicílio apresentou mediana de 4,0. Foram identificados 159 fármacos, sendo que 23 (14,5% apresentavam capacidade de induzir prolongamento do iQT. Entre os idosos, 39 (20,5% usavam estes fármacos, sendo os mais prevalentes a amiodarona, amitriptilina, nortriptilina, citalopram e fluoxetina. A hipertensão arterial foi o fator de risco mais frequente dentre aqueles que predispõem a prolongamento do iQT. As utilizações de medicamentos que induzem prolongamento do iQT e a presença de fatores de risco predisponentes mostram que os idosos estão expostos ao risco de desenvolvimento de torsade de pointes. A identificação dos fármacos que induzem prolongamento do iQT, das interações medicamentosas e das condições clínicas que predispõem a esse prolongamento são importantes para garantia da segurança da farmacoterapia de idosos e para evitar eventos adversos graves.Palavras-chave: Prolongamento do intervalo QT. Medicamentos. Idosos. Torsades de pointes. ABSTRACT The QT interval (QTi, an electrocardiographic parameter, is a noninvasive biomarker of ventricular repolarization. Increased QTi is a change that may have clinical importance because predisposes to torsade de pointes and sudden
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Efecto de la frecuencia de corte en especies patrenses. (II) Variación del contenido en nitrógeno, fósforo, potasio y azufre de Lolium perenne "V. 807" y Lolium italicum “Tetrone”
OpenAIRE
García Criado, Balbino; Duque Macías, Francisco; García Ciudad, Antonia
2011-01-01
Se estudia el efecto conjunto de la época de recolección y frecuencia de corte sobre el contenido en nitrógeno, fósforo, potasio y azufre de Lolium perenne V. 807 y Lolium italicum Tetrone. Los cortes se realizan a intervalos de diez, quince, veinte y treinta días en el período de abril a noviembre a 5-10 cm. del suelo. Se considera también la evolución de los elementos con la madurez en cortes mensuales. Los contenidos medios de nitrógeno y fósforo en Lolium perenne y de nitrógeno y azufre e...
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RT-PCR-ELISA as a tool for diagnosis of low-pathogenicity avian influenza
DEFF Research Database (Denmark)
Dybkaer, Karen; Munch, Mette; Handberg, Kurt Jensen
2003-01-01
A one-tube reverse transcriptase/polymerase chain reaction coupled with an enzyme-linked immunosorbent assay (RT-PCR-ELISA) was developed for the rapid detection of avian influenza virus (AIV) in clinical specimens. A total of 419 swab pools were analyzed from chickens experimentally infected wit...... of the twenty-three VI-positive specimens were negative when tested by RT-PCR-ELISA. The diagnostic sensitivity and specificity of the RT-PCR-ELISA was 91% and 97%, respectively, using VI in SPF eggs as the gold reference standard....
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Seguimiento posquirúrgico de los pacientes con poliposis adenomatosa familiar: resultados en una población del sur de España Follow-up after surgical treatment of patients whit familial adenomatous polyposis: Results in Southern Spanish population
Directory of Open Access Journals (Sweden)
C. Cordero Fernández
2007-08-01
Full Text Available Objetivo: analizar la evolución de la mucosa rectal y del reservorio así como idoneidad de los intervalos de seguimiento y del tratamiento realizado para evitar la aparición del cáncer, en una serie de pacientes con poliposis adenomatosa familiar (PAF, intervenidos. Método: estudio prospectivo de 28 pacientes con PAF intervenidos mediante anastomosis íleo-rectal (20 pacientes y anastomosis íleo-anal con reservorio (8 pacientes. A todos se les había realizado un control endoscópico dos veces al año y análisis del número y características macroscópicas e histológicas de los pólipos antes y después de la cirugía así como del tratamiento realizado, de sus complicaciones y de la adecuación del intervalo de seguimiento. El seguimiento medio fue de 6,47 años (DE = 4,59; rango = 0,72-16,75 años. Resultados: ninguno de los 26 pacientes que cumplimentaron correctamente el protocolo de seguimiento desarrolló cáncer. Sólo dos pacientes lo desarrollaron al 1,75 y los 3 años, respectivamente del abandono del protocolo. Los pacientes que desarrollaron adenomas durante el seguimiento fueron tratados con éxito mediante polipectomía endoscópica, salvo en dos casos que se indicó cirugía. Conclusiones: en nuestra serie, el incumplimiento de las revisiones ha sido el factor que ha condicionado la aparición de cáncer.Objective: the study was to assess changes in the rectal mucosa and pouch in a series of patients with familial adenomatous polyposis (FAP who underwent either subtotal colectomy and ileorectal anastomosis (IRA or proctocolectomy and ileal pouch-anal anastomosis (IPAA, and to evaluate the suitability of the follow-up interval and postoperative treatment employed to prevent the development of cancer. Method: this study involved 28 patients with FAP who underwent IRA (n=20 or IPAA (n=8, and were followed endoscopically over a mean period of 7.47 years. The number and both macroscopic and histological features of polyps
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Development of single step RT-PCR for detection of Kyasanur forest disease virus from clinical samples
Directory of Open Access Journals (Sweden)
Gouri Chaubal
2018-02-01
Discussion and conclusion: The previously published sensitive real time RT-PCR assay requires higher cost in terms of reagents and machine setup and technical expertise has been the primary reason for development of this assay. A single step RT-PCR is relatively easy to perform and more cost effective than real time RT-PCR in smaller setups in the absence of Biosafety Level-3 facility. This study reports the development and optimization of single step RT-PCR assay which is more sensitive and less time-consuming than nested RT-PCR and cost effective for rapid diagnosis of KFD viral RNA.
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Pärt, Arvo: Litany. Psalom. Trisagion / Robert Cowan
Index Scriptorium Estoniae
Cowan, Robert
1996-01-01
Uuest heliplaadist "Pärt, Arvo: Litany. Psalom. Trisagion. Hilliard Ensemble, Estonian Philharmonic Chamber Choir, Tallinn Chamber Orchestra, Tõnu Kaljuste; Lithuanian Chamber Orchestra, Saulius Sondeckis" ECM New Series 449 810-4; 449 810-2 (42 minutes: DDD)
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Performance of nested RT-PCR on CSF for tuberculous meningitis diagnosis in HIV-infected patients.
Science.gov (United States)
Gualberto, F A S; Gonçalves, M G; Fukasawa, L O; Santos, A M Ramos Dos; Sacchi, C T; Harrison, L H; Boulware, D R; Vidal, J E
2017-10-01
Timely diagnosis of tuberculous meningitis (TBM) in patients with human immunodeficiency virus (HIV) infection remains a challenge. Despite the current scale-up of the Xpert® MTB/RIF assay, other molecular diagnostic tools are necessary, particularly in referral centres in low- and middle-income countries without Xpert testing. To determine the diagnostic performance of nested real-time polymerase chain reaction (nRT-PCR) in HIV-infected TBM patients categorised according to standardised clinical case definitions. Based on clinical, laboratory and imaging data, HIV-infected patients with suspected TBM were prospectively categorised as 'definite TBM', 'probable TBM', 'possible TBM' or 'not TBM'. We evaluated nRT-PCR sensitivity and specificity in diagnosing TBM among definite TBM cases, and among definite + probable TBM cases. Ninety-two participants were enrolled in the study. nRT-PCR sensitivity for definite TBM (n = 8) was 100% (95%CI 67-100) and 86% (95%CI 60-96) for both definite and probable TBM (n = 6). Assuming that 'not TBM' patients (n = 74) were true-negatives, nRT-PCR specificity was 100% (95%CI 95-100). The possible TBM group (n = 4) had no nRT-PCR positives. The nRT-PCR is a useful rule-in test for HIV-infected patients with TBM according to international consensus case definitions. As nRT-PCR cannot exclude TBM, studies comparing and combining nRT-PCR with other assays are necessary for a rule-out test.
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Efecto de la sigatoka negra (Mycosphaerella fijiensis sobre la fotosíntesis y transpiración foliar del banano (Musa sp. AAA, cv. Valery
Directory of Open Access Journals (Sweden)
Martín Hidalgo
2006-01-01
Full Text Available Con el propósito de relacionar el ataque de la Sigatoka negra (Mycosphaerella fijiensis con las tasas fotosintética y de transpiración foliares, se sembraron rebrotes del tercer ciclo de producción de una plantación del cv. Valery en potes plásticos de 4 litros. Las plantas se mantuvieron dentro de un invernadero a humedad relativa y temperatura del 90% y 27°C, respectivamente. Al emitir 6 hojas, las 4 más jóvenes fueron inoculadas con conidios de M. fijiensis a partir de cultivos monospóricos de aislamientos silvestres. Dos riegos diarios por aspersión mantuvieron una película de agua sobre las hojas. Se aplicó una suspensión de 150000 conidios ml-1 sobre el envés de las hojas utilizando un aerógrafo, en 2 fechas con un intervalo de 30 días entre ellas, para obtener diferentes grados de severidad y estadios de la enfermedad en hojas de diferentes edades. Tanto la determinación visual del porcentaje de severidad como la del estadio de desarrollo de la enfermedad, fueron llevadas a cabo en los 6,25 cm2 del área foliar cubierta por la cubeta del medidor infrarrojo de gases, utilizado para determinar las tasas fotosintéticas y transpiratorias foliares. La tasa fotosintética neta foliar (Fn; μmoles de CO2 reducidos por m2 s-1 decayó con el incremento del porcentaje de severidad (Fn=-0,1517 x+6,845; R2=0,72 y el estadio de la enfermedad (Fn=-1,62 x+8,36; R2=0,60. El impacto del patógeno sobre la tasa transpiratoria foliar (E; mmoles de agua m-2 s-1 fue relativamente menor, como lo mostró la regresión de E contra el porcentaje de severidad (E=-0,0122 x+2,429; R2=0,11
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SASqPCR: robust and rapid analysis of RT-qPCR data in SAS.
Directory of Open Access Journals (Sweden)
Daijun Ling
Full Text Available Reverse transcription quantitative real-time PCR (RT-qPCR is a key method for measurement of relative gene expression. Analysis of RT-qPCR data requires many iterative computations for data normalization and analytical optimization. Currently no computer program for RT-qPCR data analysis is suitable for analytical optimization and user-controllable customization based on data quality, experimental design as well as specific research aims. Here I introduce an all-in-one computer program, SASqPCR, for robust and rapid analysis of RT-qPCR data in SAS. This program has multiple macros for assessment of PCR efficiencies, validation of reference genes, optimization of data normalizers, normalization of confounding variations across samples, and statistical comparison of target gene expression in parallel samples. Users can simply change the macro variables to test various analytical strategies, optimize results and customize the analytical processes. In addition, it is highly automatic and functionally extendable. Thus users are the actual decision-makers controlling RT-qPCR data analyses. SASqPCR and its tutorial are freely available at http://code.google.com/p/sasqpcr/downloads/list.
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Karje kunstniku südamest / Kärt Hellerma
Index Scriptorium Estoniae
Hellerma, Kärt, 1956-
1997-01-01
Arvustus: Dinesen, Isak [Blixen, Karen]. Babette'i pidusöök / tlk. Piret Peiker. Tallinn : Varrak, 1996. Ilmunud ka kogumikus: Hellerma, Kärt. Kohanenud kirjandus : valik kirjanduskriitikat 1987-2006. Eesti Keele Sihtasutus : Tallinn, 2006. Lk. 167-169
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NASA/SPoRt: GOES-R Activities in Support of Product Development, Management, and Training
Science.gov (United States)
Fuell, Kevin; Jedlovec, Gary; Molthan, Andrew; Stano, Geoffrey
2012-01-01
SPoRT is using current capabilities of MODIS and VIIRS, combined with current GOES (i.e. Hybrid Imagery) to demonstrate mesoscale capabilities of future ABI instrument. SPoRT is transitioning RGBs from EUMETSAT standard "recipes" to demonstrate a method to more efficiently handle the increase channels/frequency of ABI. Challenges for RGB production exist. Internal vs. external production, Bit depth needed, Adding quantitative information, etc. SPoRT forming group to address these issues. SPoRT is leading efforts on the application of total lightning in operations and to educate users of this new capability. Training in many forms is used to support testbed activities and is a key part to the transition process.
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El espaciamiento de los nacimientos: una estrategia para conciliar trabajo y familia en España
Directory of Open Access Journals (Sweden)
Julia Cordero
2009-01-01
Full Text Available Los intervalos entre nacimientos apenas han sido considerados un fenómeno de interés en los países desarrollados, sino una mera consecuencia de las decisiones de los individuos sobre el tamaño total de la familia y la edad al tener el primer hijo. Sin embargo, es posible que las mujeres espacien los nacimientos de forma estratégica para hacer que el empleo y la maternidad sean más compatibles. El objetivo de este artículo es arrojar luz sobre el análisis de la influencia que el apego a trabajar fuera del hogar por parte de las mujeres y el hecho de recibir ayuda con las tareas domésticas pueden ejercer sobre la duración del intervalo entre el primer nacimiento y el segundo. Para ello, tomo la información de una muestra de 871 mujeres españolas con edades entre los 35 y los 49 años. Los datos han sido extraídos de la Encuesta de Fecundidad y Familia para España, llevada a cabo en 1995. Los resultados obtenidos a través de modelos de regresión lineal multivariada indican que un fuerte apego al trabajo fuera del hogar afecta negativamente sobre la duración del intervalo entre los dos primeros nacimientos, en especial entre mujeres con un nivel alto de educación. No obstante, el efecto positivo de recibir ayuda con las tareas domésticas puede contrarrestar la influencia de las características mencionadas.
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Quantitative RT-PCR for titration of replication-defective recombinant Semliki Forest virus.
Science.gov (United States)
Puglia, Ana L P; Rezende, Alexandre G; Jorge, Soraia A C; Wagner, Renaud; Pereira, Carlos A; Astray, Renato M
2013-11-01
Virus titration may constitute a drawback in the development and use of replication-defective viral vectors like Semliki Forest virus (SFV). The standardization and validation of a reverse transcription quantitative PCR (qRT-PCR) method for SFV titration is presented here. The qRT-PCR target is located within the nsp1 gene of the non-structural polyprotein SFV region (SFV RNA), which allows the strategy to be used for several different recombinant SFV constructs. Titer determinations were carried out by performing virus titration and infection assays with SFVs containing an RNA coding region for the rabies virus glycoprotein (RVGP) or green fluorescent protein (GFP). Results showed that the standardized qRT-PCR is applicable for different SFV constructs, and showed good reproducibility. To evaluate the correlation between the amount of functional SFV RNA in a virus lot and its infectivity in BHK-21 cell cultures, a temperature mediated titer decrease was performed and successfully quantitated by qRT-PCR. When used for cell infection at the same multiplicity of infection (MOI), the temperature treated SFV-RVGP samples induced the same levels of RVGP expression. Similarly, when different SFV-GFP lots with different virus titers, as accessed by qRT-PCR, were used for cell infection at the same MOI, the cultures showed comparable amounts of fluorescent cells. The data demonstrate a good correlation between the amount of virus used for infection, as measured by its SFV RNA, and the protein synthesis in the cells. In conclusion, the qRT-PCR method developed here is accurate and enables the titration of replication-defective SFV vectors, an essential aid for viral vector development as well as for establishment of production bioprocesses. Copyright © 2013 Elsevier B.V. All rights reserved.
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Study of rt-PA therapy for acute stroke in older patients
International Nuclear Information System (INIS)
Ono, Yasuhiro; Toyoshima, Atsuhiko; Toyota, Yasunori; Kuramoto, Satoshi; Katsumata, Atsushi; Kawauchi, Masamitsu; Matsumoto, Yuzo
2011-01-01
Intravenous alteplase (rt-PA) therapy has been widely approved for acute ischemic stroke. We examined symptomatic intracranial hemorrhage (SICH) and outcomes in older patients treated with rt-PA therapy. We divided 130 consecutive patients treated with rt-PA into two groups: 72 patients younger than 80 years (age <80 group) and 58 patients older than 80 years (age ≥80 group). On CT and MRI scans, SICH was observed in 5 patients (7.1%) in the age <80 group and 4 patients (6.9%) in the age ≥80 group. The SICH rate did not differ significantly between the two groups (odds ratio (OR) 1.21, 95% confidence interval (CI) 0.04-2.39, P=0.6174). The patients in the age ≥80 group had a significantly lower mRS 0-1 rate (8.8 vs. 30.0%; OR 7.20, 95% CI 6.05-8.34, P=0.0009) and significantly higher mRS 6 (mortality) rate (19.3 vs. 9.0%; OR 6.52, 95% CI 5.39-7.65, P=0.0015) compared with those in the age <80 group. These data suggest that the factor of age is not related to SICH but is related to patients' outcomes in rt-PA therapy. (author)
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GOKaRT: Graphical Online Search Tool for Maps
Directory of Open Access Journals (Sweden)
Mechthild Schüler
2008-09-01
Full Text Available The map department of the Staats- und Universitätsbibliothek Göttingen together with the Berlin State Library propose a project to develop a web-based graphic cataloguing and search system for maps, to be funded by the German Research Foundation. This tool shall be made available to all map holdings in archives, libraries, university departments and museums in Germany as a comfortable means for the administration of map holdings and as a search tool. Sheets belonging to map series as well as single maps (old and new will be registered cooperatively by the participants with simple tools. This cooperation in data maintenance will facilitate the work especially for understaffed map holdings. Depending on the type of map there are four different mechanisms for map reference. For map series electronic index sheets are used which will show information regarding the various issues of the map sheets. Due to the intuitive graphic search entry GOKaRT-users will easily find the required maps of a certain region available in a chosen holding. User administration modules ensure comfortable handling. GOKaRT is being developed on the basis of licence-free open source programmes. In case financing is provided by the German Research Foundation, GOKaRT can be used free of charge internationally. This would require a contract stipulating data exchange between the partners as well as permanent storage and usability of the data.
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Data acquisition and real-time bolometer tomography using LabVIEW RT
International Nuclear Information System (INIS)
Giannone, L.; Eich, T.; Fuchs, J.C.; Ravindran, M.; Ruan, Q.; Wenzel, L.; Cerna, M.; Concezzi, S.
2011-01-01
The currently available multi-core PCI Express systems running LabVIEW RT (real-time), equipped with FPGA cards for data acquisition and real-time parallel signal processing, greatly shorten the design and implementation cycles of large-scale, real-time data acquisition and control systems. This paper details a data acquisition and real-time tomography system using LabVIEW RT for the bolometer diagnostic on the ASDEX Upgrade tokamak (Max Planck Institute for Plasma Physics, Garching, Germany). The transformation matrix for tomography is pre-computed based on the geometry of distributed radiation sources and sensors. A parallelized iterative algorithm is adapted to solve a constrained linear system for the reconstruction of the radiated power density. Real-time bolometer tomography is performed with LabVIEW RT. Using multi-core machines to execute the parallelized algorithm, a cycle time well below 1 ms is reached.
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DaRT: A CALL System to Help Students Practice and Develop Reasoning Skills in Choosing English Articles.
Science.gov (United States)
Yoshii, Rika; Milne, Alastair
1998-01-01
Describes DaRT, a computer assisted language-learning system for helping English-as-a-Second-Language students master English articles. DaRT uses a diagrammatic reasoning tool to present communicative contexts for exercises in choosing appropriate articles. This paper describes the development of DaRT and DaRT's system components and concludes…
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Thermal regulation 2000: one year later; RT 2000: un an apres
Energy Technology Data Exchange (ETDEWEB)
NONE
2002-05-01
This meeting, organized by the research center of Gaz de France (Cegibat), aims at drawing up a progress status of the French thermal regulation (RT 2000), one year after its enforcement on June 1, 2000: status, experience feedback and recommendations; Gaz de France assistance and solutions; tools at the disposition of designers (labelling of equipments, simulation softwares..); practical examples of implementation of the RT 2000 in office buildings, residential buildings and sport complexes. (J.S.)
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Caracterización nutricional del cactus nopal (Opuntia ficus-indica bajo diferentes tratamientos de fertilización
Directory of Open Access Journals (Sweden)
Carlos Eduardo Rodríguez-M.
2011-02-01
Full Text Available Nuestro planeta se enfrenta actualmente a grandes cambios en su ambiente natural, no sólo como consecuencia de su evolución, sino también del acelerado proceso de degradación antrópica. Dentro de este contexto se origina la necesidad de buscar soluciones que mitiguen el bajo nivel de vida del campesino, dadas las precarias condiciones de muchos de los actuales ecosistemas, que no permiten implementar los modelos tradicionales de explotación desmesurada de los recursos. El cactus nopal (Opuntia ficus-indica ofrece ventajas en la producción animal, presentándose como una alternativa agroecológica, fácil de implementar en zonas semiáridas bajo condiciones adversas y con un mínimo de insumosagropecuarios. El objetivo de este estudio fue evaluar el desempeño nutricional del cactus nopal bajo cuatro tipos de fertilización, mediante el análisis proximal de alimentos, implementado en el laboratorio de nutrición animal de la Universidad Pedagógica y Tecnológica de Colombia, UPTC sede Tunja, en los especímenes de cactus nopal existentes en el jardín botánico de la misma. Se utilizaron cuatro tipos de tratamientos para la fertilización: 1.Químico, 2.Orgánico, 3. Químico + Orgánico y 4.Testigo, aplicados en intervalos de veinte (20 días, por tres veces, aplicando también agua en un riego semanal; tiempo al cabo del cual se realizó la valoraciónnutricional, en donde el Tratamiento 2 (Orgánico fue el de mejor desempeño, seguido por el Tratamiento 3, luego el tratamiento 1 y, por último, el tratamiento 4 (testigo.
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The effects of inspiratory muscle strengthening on MIP and quality of life of elderly nursing home patients. (Los efectos del beneficio de la fuerza del músculo inspiratorio en la PIM y la calidad de la vida de los pacientes mayores de la clínica de reposo.
Directory of Open Access Journals (Sweden)
Silvia Correa Bacelar
2008-01-01
Full Text Available AbstractThe objective of this study was to compare the effects of inspiratory muscle strengthening on maximal inspiratory pressure (MIP and on quality of life among elderly nursing home patients. The study population was divided in two groups: experimental group- EG (n=21, 76.48 2.12 years and control group- CG (n=13, 75.69 2.26 years. The World Health Organization Quality of Life Group (WHOQOL-100 questionnaire was used to evaluate the quality of life of the subjects. A Manovacuometer (analogical, with an operational interval of –150 to +150 cmH2O; Critical Med/USA-2002 was used to evaluate MIP. The study protocol consisted of a gradual increase in workload (50%-100%; the sessions lasted 20 minutes and were composed of 7 series of strengthening (2 minutes each with a 1-minute interval between the series, 3 times a week for 10 weeks. Variance analysis of multivariate repeated measurements demonstrated significant differences only for MIP between CG (32,69±17,03 cmH2O versus 23,08±10,71 cmH2O and EG (31,67±11,11 cmH2O versus 55,24±23,26 cmH2O, and the latter was higher than the former (p= 0,00000. Therefore, we concluded that inspiratory muscle strengthening led to an increase in MIP that did not improve quality of life. However, further studies should be done in this population to validate a quality of life questionnaire specific for the elderly.ResumenEl objetivo de este estudio era comparar los efectos del músculo inspiratorio que consolidaban en la presión inspiratoria máxima (PIM y en la calidad de la vida entre pacientes mayores de la clínica de reposo. Dividieron a la población del estudio en dos grupos: grupo experimental EG. (n=21,76.48 2.12 años y grupo de control CG (n=13, 75.69 2.26 años. La calidad de la Organización Mundial de la Salud del cuestionario del grupo de la vida(WHOQOL-100 fue utilizada para evaluar la calidad de la vida de los temas. Un Manovacuometer (analógico, con un intervalo operacional - de 150 a
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L. Harrison: Mass (to St. Anthony); A. Pärt: Berliner Messe / Robert Cowan
Index Scriptorium Estoniae
Cowan, Robert
1994-01-01
Uuest heliplaadist "L. Harrison: Mass (to St. Anthony); A. Pärt: Berliner Messe. Oregon Repertory Singers / Gilbert Seeley. Koch International Classics CD 37 177-2; Pärt - comparative version: Estonian Phil. Chbr. Ch., Tallinn CO / Kaljuste" (11/93)(ECM) 439 162-2
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Investigating the Decision-Making of Response to Intervention (RtI) Teams within the School Setting
Science.gov (United States)
Thur, Scott M.
2015-01-01
The purpose of this study was to measure decision-making influences within RtI teams. The study examined the factors that influence school personnel involved in three areas of RtI: determining which RtI measures and tools teams select and implement (i.e. Measures and Tools), evaluating the data-driven decisions that are made based on the…
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WE-H-207B-04: Strategies for Adaptive RT
Energy Technology Data Exchange (ETDEWEB)
Green, O. [Washington University School of Medicine (United States)
2016-06-15
In recent years, steady progress has been made towards the implementation of MRI in external beam radiation therapy for processes ranging from treatment simulation to in-room guidance. Novel procedures relying mostly on MR data are currently implemented in the clinic. This session will cover topics such as (a) commissioning and quality control of the MR in-room imagers and simulators specific to RT, (b) treatment planning requirements, constraints and challenges when dealing with various MR data, (c) quantification of organ motion with an emphasis on treatment delivery guidance, and (d) MR-driven strategies for adaptive RT workflows. The content of the session was chosen to address both educational and practical key aspects of MR guidance. Learning Objectives: Good understanding of MR testing recommended for in-room MR imaging as well as image data validation for RT chain (e.g. image transfer, filtering for consistency, spatial accuracy, manipulation for task specific); Familiarity with MR-based planning procedures: motivation, core workflow requirements, current status, challenges; Overview of the current methods for the quantification of organ motion; Discussion on approaches for adaptive treatment planning and delivery. T. Stanescu - License agreement with Modus Medical Devices to develop a phantom for the quantification of MR image system-related distortions.; T. Stanescu, N/A.
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WE-H-207B-02: MR-Driven RT Planning
Energy Technology Data Exchange (ETDEWEB)
Cao, M. [UCLA School of Medicine (United States)
2016-06-15
In recent years, steady progress has been made towards the implementation of MRI in external beam radiation therapy for processes ranging from treatment simulation to in-room guidance. Novel procedures relying mostly on MR data are currently implemented in the clinic. This session will cover topics such as (a) commissioning and quality control of the MR in-room imagers and simulators specific to RT, (b) treatment planning requirements, constraints and challenges when dealing with various MR data, (c) quantification of organ motion with an emphasis on treatment delivery guidance, and (d) MR-driven strategies for adaptive RT workflows. The content of the session was chosen to address both educational and practical key aspects of MR guidance. Learning Objectives: Good understanding of MR testing recommended for in-room MR imaging as well as image data validation for RT chain (e.g. image transfer, filtering for consistency, spatial accuracy, manipulation for task specific); Familiarity with MR-based planning procedures: motivation, core workflow requirements, current status, challenges; Overview of the current methods for the quantification of organ motion; Discussion on approaches for adaptive treatment planning and delivery. T. Stanescu - License agreement with Modus Medical Devices to develop a phantom for the quantification of MR image system-related distortions.; T. Stanescu, N/A.
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WE-H-207B-04: Strategies for Adaptive RT
International Nuclear Information System (INIS)
Green, O.
2016-01-01
In recent years, steady progress has been made towards the implementation of MRI in external beam radiation therapy for processes ranging from treatment simulation to in-room guidance. Novel procedures relying mostly on MR data are currently implemented in the clinic. This session will cover topics such as (a) commissioning and quality control of the MR in-room imagers and simulators specific to RT, (b) treatment planning requirements, constraints and challenges when dealing with various MR data, (c) quantification of organ motion with an emphasis on treatment delivery guidance, and (d) MR-driven strategies for adaptive RT workflows. The content of the session was chosen to address both educational and practical key aspects of MR guidance. Learning Objectives: Good understanding of MR testing recommended for in-room MR imaging as well as image data validation for RT chain (e.g. image transfer, filtering for consistency, spatial accuracy, manipulation for task specific); Familiarity with MR-based planning procedures: motivation, core workflow requirements, current status, challenges; Overview of the current methods for the quantification of organ motion; Discussion on approaches for adaptive treatment planning and delivery. T. Stanescu - License agreement with Modus Medical Devices to develop a phantom for the quantification of MR image system-related distortions.; T. Stanescu, N/A
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WE-H-207B-02: MR-Driven RT Planning
International Nuclear Information System (INIS)
Cao, M.
2016-01-01
In recent years, steady progress has been made towards the implementation of MRI in external beam radiation therapy for processes ranging from treatment simulation to in-room guidance. Novel procedures relying mostly on MR data are currently implemented in the clinic. This session will cover topics such as (a) commissioning and quality control of the MR in-room imagers and simulators specific to RT, (b) treatment planning requirements, constraints and challenges when dealing with various MR data, (c) quantification of organ motion with an emphasis on treatment delivery guidance, and (d) MR-driven strategies for adaptive RT workflows. The content of the session was chosen to address both educational and practical key aspects of MR guidance. Learning Objectives: Good understanding of MR testing recommended for in-room MR imaging as well as image data validation for RT chain (e.g. image transfer, filtering for consistency, spatial accuracy, manipulation for task specific); Familiarity with MR-based planning procedures: motivation, core workflow requirements, current status, challenges; Overview of the current methods for the quantification of organ motion; Discussion on approaches for adaptive treatment planning and delivery. T. Stanescu - License agreement with Modus Medical Devices to develop a phantom for the quantification of MR image system-related distortions.; T. Stanescu, N/A
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Simultaneous detection of enteropathogenic viruses in buffalos faeces using multiplex reverse transcription-polymerase chain reaction (mRT-PCR
Directory of Open Access Journals (Sweden)
U. Pagnini
2010-02-01
Full Text Available A multiplex reverse transcription- polymerase chain reaction (mRT-PCR assay that detects Bovine Viral Diarrhoea Virus, Bovine Coronavirus, and Group A Rotaviruses in infected cell-culture fluids and clinical faecal samples is described. One hundred twenty faecal samples from buffalo calves with acute gastroenteritis were tested. The mRT-PCR was validated against simplex RT-PCR with published primers for Pestivirus, Coronavirus and Rotavirus. The multiplex RT-PCR was equally sensitive and specific in detecting viral infections compared with simplex RT-PCR. The mRT-PCR readily identified viruses by discriminating the size of their amplified gene products. This mRT-PCR may be a sensitive and rapid assay for surveillance of buffalo enteric viruses in field specimens. This novel multiplex RT-PCR is an attractive technique for the rapid, specific, and cost-effective laboratory diagnosis of acute gastroenteritis.
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Polar S810 como recurso alternativo ao eletrocardiograma no teste de exercício de 4 segundos Polar S810 como recurso alternativo al electrocardiograma en la prueba de ejercicio de 4 segundos Polar S810 as an alternative resource to the use of the electrocardiogram in the 4-second exercise test
Directory of Open Access Journals (Sweden)
Alan Santos Pimentel
2010-05-01
Full Text Available FUNDAMENTO: O teste de exercício de 4 segundos (T4s avalia o tônus vagal cardíaco durante o transiente inicial da frequência cardíaca (FC, em exercício dinâmico súbito, por meio da identificação do índice vagal cardíaco (IVC obtido a partir do eletrocardiograma (ECG. OBJETIVO: Testar a utilização do monitor de frequência cardíaca (MFC Polar S810 como recurso alternativo ao ECG na aplicação do T4s. MÉTODOS: Neste trabalho, 49 indivíduos do sexo masculino (25 ± 20 anos, 176 ± 12 cm, 74 ± 6 kg realizaram o T4s. Os intervalos RR foram registrados simultaneamente por ECG e MFC. Calcularam-se média e desvio padrão do último intervalo RR do período pré-exercício ou o primeiro do período de exercício, aquele que for mais longo (RRB, do mais curto intervalo RR do período de exercício (RRC e do IVC obtidos por ECG e MFC. Utilizou-se o teste t de Student para amostras dependentes (p FUNDAMENTO: El test de ejercicio de 4 segundos (T4s evalúa el tono vagal cardíaco durante el transiente inicial de la frecuencia cardíaca (FC, en ejercicio dinámico súbito, por medio de la identificación del índice vagal cardíaco (IVC obtenido a partir del electrocardiograma (ECG. OBJETIVO: Probar la utilización del monitor de frecuencia cardiaca (MFC Polar S810 como recurso alternativo al ECG en la aplicación del T4s. MÉTODOS: En este trabajo, 49 individuos del sexo masculino (25 ± 20 años, 176 ±12 cm, 74 ± 6 kg llevaron el T4s. Los intervalos RR fueron registrados simultáneamente por ECG y MFC. Se calcularon el promedio y la desviación estándar del último intervalo RR del período pre ejercicio o el primer del período de ejercicio, aquel que fuera más largo (RRB, del más corto intervalo RR del período de ejercicio (RRC y del IVC obtenidos por ECG y MFC. Se utilizó la prueba t de Student para muestras dependientes (P BACKGROUND: The 4-second exercise test (T4s evaluates the cardiac vagal tone during the initial heart
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SU-F-J-219: Predicting Ventilation Change Due to Radiation Therapy: Dependency On Pre-RT Ventilation and Effort Correction
Energy Technology Data Exchange (ETDEWEB)
Patton, T; Du, K; Bayouth, J [University of Wisconsin, Madison, WI (United States); Christensen, G; Reinhardt, J [University of Iowa, Iowa City, IA (United States)
2016-06-15
Purpose: Ventilation change caused by radiation therapy (RT) can be predicted using four-dimensional computed tomography (4DCT) and image registration. This study tested the dependency of predicted post-RT ventilation on effort correction and pre-RT lung function. Methods: Pre-RT and 3 month post-RT 4DCT images were obtained for 13 patients. The 4DCT images were used to create ventilation maps using a deformable image registration based Jacobian expansion calculation. The post-RT ventilation maps were predicted in four different ways using the dose delivered, pre-RT ventilation, and effort correction. The pre-RT ventilation and effort correction were toggled to determine dependency. The four different predicted ventilation maps were compared to the post-RT ventilation map calculated from image registration to establish the best prediction method. Gamma pass rates were used to compare the different maps with the criteria of 2mm distance-to-agreement and 6% ventilation difference. Paired t-tests of gamma pass rates were used to determine significant differences between the maps. Additional gamma pass rates were calculated using only voxels receiving over 20 Gy. Results: The predicted post-RT ventilation maps were in agreement with the actual post-RT maps in the following percentage of voxels averaged over all subjects: 71% with pre-RT ventilation and effort correction, 69% with no pre-RT ventilation and effort correction, 60% with pre-RT ventilation and no effort correction, and 58% with no pre-RT ventilation and no effort correction. When analyzing only voxels receiving over 20 Gy, the gamma pass rates were respectively 74%, 69%, 65%, and 55%. The prediction including both pre- RT ventilation and effort correction was the only prediction with significant improvement over using no prediction (p<0.02). Conclusion: Post-RT ventilation is best predicted using both pre-RT ventilation and effort correction. This is the only prediction that provided a significant
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Kaks vaadet Ameerikast Jacques Derridale / Märt Väljataga
Index Scriptorium Estoniae
Väljataga, Märt, 1965-
1998-01-01
Kommentaariks Vikerkaares 1998, nr. 7-8 ilmunud artiklitele: Mark Lilla 'Jacques Derrida poliitika' ja Richard Rorty 'Ironistlikust teooriast privaatsete allusioonideni' (mõlemad tõlkinud Märt Väljataga)
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Is tumor necrosis factor - 376a promoter polymorphism associated with susceptibility to multiple sclerosis? ¿El polimorfismo-376A del promotor del gen del factor de necrosis tumoral se asocia con una mayor susceptibilidad a padecer esclerosis múltiple?
Directory of Open Access Journals (Sweden)
Marcelo A. Kauffman
2007-10-01
Full Text Available A single nucleotide polymorphism (SNP at position -376 of the tumor necrosis factor á gene (TNFA has been associated with susceptibility to multiple sclerosis (MS in Spain. However, no association was found in populations from the USA and The Netherlands. Here we investigate the association between the TNFA - 376A SNP and MS susceptibility in Argentinean patients with MS. The A/G genotype was found in 4.4% of patients (n=90 and in 4.8% of healthy individuals (n=84; p=0.92; odds ratio=0.93; confidence interval: 0.23- 3.84. Thus, no significant differences in genotype and allele frequencies were found between healthy individuals and patients with MS in Argentina.Un polimorfismo de nucleótido único (SNP, por sus iniciales en inglés en la posición -376 del gen codificante del factor de necrosis tumoral á (TNFA ha sido asociado en España con un mayor riesgo a padecer esclerosis múltiple (EM. Sin embargo, esta asociación no fue encontrada en estudios hechos en poblaciones provenientes de los EE.UU. y Holanda. Aquí investigamos la asociación entre el SNP TNFA -376A y el desarrollo de EM en una población de pacientes argentinos con EM. El genotipo A/G fue encontrado en 4.4% de los pacientes (n=90 y en 4.8% de los controles sanos (n=84; p=0.92; odds ratio=0.93; intervalo de confianza: 0.23-3.84. En consecuencia, no encontramos diferencias en las frecuencias alélicas y genotípicas entre los sujetos enfermos y los controles sanos en Argentina.
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Udtrækning af 10 mm ankerbolte
DEFF Research Database (Denmark)
Hansen, Lars Pilegaard
Denne rapport beskriver forsøg med udtrækning af 10 mm ankerbolte udført af Laboratoriet for Bærende Konstruktioner, Instituttet for Bygningsteknik, Aalborg Universitet. Forsøgene blev udført d. 19. og 21. juni 2001. Forsøgene blev udført dels på Hjørring Sygehus og dels på Laboratoriet.......Denne rapport beskriver forsøg med udtrækning af 10 mm ankerbolte udført af Laboratoriet for Bærende Konstruktioner, Instituttet for Bygningsteknik, Aalborg Universitet. Forsøgene blev udført d. 19. og 21. juni 2001. Forsøgene blev udført dels på Hjørring Sygehus og dels på Laboratoriet....
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Real-time RT-PCR, a necessary tool to support the diagnosis and surveillance of rotavirus in Mexico.
Science.gov (United States)
De La Cruz Hernández, Sergio Isaac; Anaya Molina, Yazmin; Gómez Santiago, Fabián; Terán Vega, Heidi Lizbeth; Monroy Leyva, Elda; Méndez Pérez, Héctor; García Lozano, Herlinda
2018-04-01
Rotavirus produces diarrhea in children under 5 years old. Most of those conventional methods such as polyacrylamide gel electrophoresis (PAGE) and reverse transcription-polymerase chain reaction (RT-PCR) have been used for rotavirus detection. However, these techniques need a multi-step process to get the results. In comparison with conventional methods, the real-time RT-PCR is a highly sensitive method, which allows getting the results in only one day. In this study a real-time RT-PCR assay was tested using a panel of 440 samples from patients with acute gastroenteritis, and characterized by PAGE and RT-PCR. The results show that the real-time RT-PCR detected rotavirus from 73% of rotavirus-negative samples analyzed by PAGE and RT-PCR; thus, the percentage of rotavirus-positive samples increased to 81%. The results indicate that this real-time RT-PCR should be part of a routine analysis, and as a support of the diagnosis of rotavirus in Mexico. Copyright © 2017 Elsevier Inc. All rights reserved.
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SPoRT's Participation in the GOES-R Proving Ground Activity
Science.gov (United States)
Jedlovec, Gary; Fuell, Kevin; Smith, Matthew; Stano, Geoffrey; Molthan, Andrew
2011-01-01
The next generation geostationary satellite, GOES-R, will carry two new instruments with unique atmospheric and surface observing capabilities, the Advanced Baseline Imager (ABI) and the Geostationary Lightning Mapper (GLM), to study short-term weather processes. The ABI will bring enhanced multispectral observing capabilities with frequent refresh rates for regional and full disk coverage to geostationary orbit to address many existing and new forecast challenges. The GLM will, for the first time, provide the continuous monitoring of total lightning flashes over a hemispherical region from space. NOAA established the GOES-R Proving Ground activity several years ago to demonstrate the new capabilities of these instruments and to prepare forecasters for their day one use. Proving Ground partners work closely with algorithm developers and the end user community to develop and transition proxy data sets representing GOES-R observing capabilities. This close collaboration helps to maximize refine algorithms leading to the delivery of a product that effectively address a forecast challenge. The NASA Short-term Prediction Research and Transition (SPoRT) program has been a participant in the NOAA GOES-R Proving Ground activity by developing and disseminating selected GOES-R proxy products to collaborating WFOs and National Centers. Established in 2002 to demonstrate the weather and forecasting application of real-time EOS measurements, the SPoRT program has grown to be an end-to-end research to operations activity focused on the use of advanced NASA modeling and data assimilation approaches, nowcasting techniques, and unique high-resolution multispectral data from EOS satellites to improve short-term weather forecasts on a regional and local scale. Participation in the Proving Ground activities extends SPoRT s activities and taps its experience and expertise in diagnostic weather analysis, short-term weather forecasting, and the transition of research and experimental
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Biología reproductiva del pargo Lutjanus inermis (Perciformes: Lutjanidae, en el Pacífico central mexicano
Directory of Open Access Journals (Sweden)
Gabriela Lucano-Ramírez
2012-03-01
Full Text Available Debido a los escasos antecedentes del efecto de las pesquerías y manejo del pargo Lutjanus inermis, es importante conocer la época reproductiva para el establecimiento de temporadas de veda, ya que la biomasa de los peces en fase de reproducción, es usada como punto de referencia para evaluar la condición del stock explotado y para establecer futuros niveles de captura. Por ello, el objetivo de este trabajo fue analizar algunos aspectos reproductivos como proporción sexual, época reproductiva, talla de madurez de L. inermis en las costas de Jalisco, México. Durante 1999-2008, se llevaron a cabo muestreos mensuales de los ejemplares capturados por la pesca artesanal en las bahías de Navidad, Tenacatita y Chamela (Jalisco, México. Se recolectaron 311 (53.1% hembras y 275 (46.9% machos y, la proporción de sexos (1:0.88 no presentó diferencia significativa. El intervalo de longitud total fue 14.4 a 37.0cm y el peso total de 48 a 575g. L. inermis presentó los máximos valores del IGS en dos periodos, febrero-abril y septiembre-noviembre, también en estos periodos se presentaron los mayores porcentajes de organismos en estadio de maduración. El ovario presentó el tipo de desarrollo asincrónico y se reconocieron siete fases del desarrollo de los ovocitos. En los testículos se observó el tipo de desarrollo lobular, se observaron espermatozoides en el centro de los cistos y en el conducto recolector. La talla de madurez (L50 fue de 23.9cm LT para hembras y 23.6cm LT para machos. Considerando el periodo de muestreo de este estudio, los antecedentes descritos podrían ser de utilidad en la formulación de planes o estrategias de manejo de esta especie.
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Infección nosocomial en la terapia intensiva neonatal. Factores de riesgo. Pinar del Río 2001-2003 Nosocomial infection in the neonatal intensive care unit. Risk factors, Pinar del Río
Directory of Open Access Journals (Sweden)
Ivón Aimé Sánchez Monterrey
2007-03-01
Full Text Available Con el objetivo de evaluar los factores de riesgo de las infecciones nosocomiales en la Unidad de Terapia Intensiva Neonatal del Hospital General Universitario "Abel Santamaría Cuadrado" de Pinar del Río, se realizó una investigación observacional, analítica, de tipo caso-control, para ello se estudiaron 170 niños ingresados en esta unidad en el período comprendido de abril de 2001 a diciembre de 2003. Se recogió en una planilla tipo y momento de adquisición de la infección, abordaje vascular, ventilación mecánica y gérmenes aislados, entre otros. Se elaboró una base de datos en Microsoft Excel-97, se aplicaron los Test de Chi cuadrado y test de Student, ambos con un intervalo de confianza de p With the purpose of evaluating risk factors of nosocomial infections in the Intensive Care Unit of the "Abel Santamaría Cuadrado" General Hospital in Pinar del Río, an observational, analytical and a case-control research was performed in 170 children admitted at this Unit from April 1st., 2001, to December 31st., 2003. The nosocomial infection and time of being infected were recorded in a form, as well as the vascular approach, artificial ventilation and isolated germs, among others. A data base using the Microsoft Excel 97 software was designed, and chi-square test was used, both of them with a confidence interval of p<0,05. It was observed that birth weight, sex, gestational age, stay at ICU, artificial ventilation and deep vascular approach were related significantly with the onset of the nosocomial infection versus a control group showing similar characteristics. E. Coli and negative coagulase were the most frequent isolated germs.
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La repolarización precoz en el electrocardiograma del futbolista: Prevalencia, características y evolución
Directory of Open Access Journals (Sweden)
Roberto M Peidro
2014-12-01
Full Text Available La repolarización precoz (RP en el electrocardiograma (ECG del deportista ha sido considerada un hallazgo benigno. Algunas publicaciones la han asociado a incremento de riesgo de muerte súbita. Los objetivos del presente trabajo fueron evaluar la prevalencia de RP inferolateral en una población de futbolistas entrenados, describir variables asociadas a esta patente e investigar la aparición de eventos en el seguimiento. Se analizaron ECG de futbolistas sin antecedentes personales y familiares de cardiopatía, con examen físico cardiovascular normal. Se consideró RP a la elevación del punto J ≥ 0.1 mV sobre la línea de base en, al menos, 2 derivaciones inferiores y/o precordiales laterales, asociada o no a deflexión positiva o muesca en la parte final del QRS. Se relacionaron con RP el voltaje de R en V5 o V6, el intervalo PR, la frecuencia cardíaca (FC y la edad. Fueron contactados a los 60±5 meses de la evaluación inicial con ECG. Estadística: chi cuadrado y test t para datos no apareados. Fueron evaluados 210 ECG de futbolistas hombres, de origen europeo-sudamericano, con entrenamiento de alta intensidad. Edad: 18 ±4.6 años. La RP se encontró en 86 ECG (40.9%, incluida el subtipo inferior en 17 (8.1%. Un ECG mostró RP en cara inferior en forma aislada. Los futbolistas con RP tuvieron mayor edad, menor FC, PR más prolongado y menor voltaje de R. Ninguno de los futbolistas presentó eventos cardiovasculares a los 5 años de la evaluación.
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Monitoreo de los niveles de ruido ambiental en el barrio el Poblado aplicando la técnica geoestadística del kriging poligonal
Directory of Open Access Journals (Sweden)
Sánchez A. Luís Hernán
2011-11-01
Full Text Available Este artículo contiene los resultados del monitoreo del ruido ambiental (RA sobre la franja horaria diurna (LEQD en los barrios El Poblado (14 muestras y Lalinde (2 muestras, del área urbana del municipio de Medellín. El muestreo fue realizado durante 7 días en el mes de julio de 2008 y la información fue evaluada en el Centro de Geoestadística de la Universidad Nacional de Colombia.
El análisis estructural de la variable ruido ambiental se basó en la regionalización de 417 datos tomados en el área urbana del municipio de Medellín, para el proyecto "Mapas acústicos y de concentración de monóxido de carbono de los municipios del área metropolitana del valle de Aburrá". La estructura variográfica fue acoplada a un modelo cúbico con rango de influencia de 780 metros y una desviación estándar real de 4.45 dB(A, la cual representa el 97% de la varianza teórica o estadística. Con base en estos resultados, se estimó para el barrio El Poblado utilizando la técnica del Kriging Poligonal, el nivel de RA entre 65 y 69 dB(A con un error relativo del 4%, que permite considerar geoestadísticamente este nivel de ruido en la categoría de probado. Por otro lado, el nivel de RA estimado para el barrio Lalinde podría variar en el intervalo 66 y 74 dB(A, con un error relativo del 38%, categorizado como un nivel de RA Posible; este último resultado indica que no es suficiente una muestra para categorizar el nivel acústico.
El nivel de RA máximo estimado permite considerar el barrio El Poblado en el estándar de RA (decreto 0627/2006 adecuado para un Sector C y apropiado para zonas con usos permitidos comerciales, como centros comerciales, almacenes, locales o instalaciones de tipo comercial, talleres de mecánica automotriz e industrial, centros deportivos y recreativos, gimnasios, restaurantes, bares, tabernas, discotecas, bingos, casinos.
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Prevalencia y factores de riesgo de las lesiones de la mucosa oral en la población urbana del Uruguay
Directory of Open Access Journals (Sweden)
Beatriz Casnati
Full Text Available Objetivo: El objetivo de este estudio fue determinar la prevalencia de las lesiones de la mucosa bucal e identificar los factores de riesgo en el desarrollo de las mismas en una muestra representativa de la población adulta urbana del Uruguay. Metodología: Se trata de un estudio transversal que registró 922 personas (537 F/385M, edades de 15-24, 35-44 y 65-74 años basado en un diseño muestral estratificado por conglomerado polietápico. A partir de los datos de la muestra se estimaron las prevalencias de cada entidad así como los intervalos de confianza al 95%. Resultados: La candidosis y las lesiones proliferativas se observaron en 26% y en 17% de las personas y se presentaron significativamente asociadas al género femenino. La prevalencia de la leucoplasia fue del 7% y en el análisis multivariado presentó una asociación significativa con el consumo de mate. Conclusiones: Las lesiones orales se presentan de manera prevalente en los adultos mayores en el Uruguay, lo que sugiere que se deberían implementar programas de prevención, diagnóstico y tratamiento adecuado de las mismas
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La actividad física, el entrenamiento continuo e intervalo: una solución para la salud
Directory of Open Access Journals (Sweden)
Ricardo Ortiz-Pulido
2017-01-01
Full Text Available El propósito de este documento fue reportar los beneficios de la actividad física, entrenamientointervalo y entrenamiento continuo moderado en adultos sedentarios y físicamente activos.La actividad física involucra cualquier movimiento corporal que produce un aumento en elgasto energético en el metabolismo, mientras que el entrenamiento intervalo y entrenamientocontinuo moderado puede ser utilizado para controlar el programa de cargas de entrenamiento(intensidad, volumen y pausa. Los beneficios que se han reportado cuando se realiza actividadfísica son: el incremento o mantenimiento de la condición física muscular, funciones cognitivas,cardiorespiratoria, equilibrio, peso corporal, control de la obesidad; todos ellos disminuyen losriesgos de enfermedades cardiovasculares, enfernedades crónicorrespiratorias, diabetes, presiónalterial alta, sindrome metabolico, cáncer de colon, depresión y todas las causas de mortalidad.En contraste, la falta de actividad fisica ha ha sido identificada como factor de riesgo y estáasociada a diversas enfemedades no transmisibles a nivel mundial. En este documento puntua-lizamos dos tipos de entrenamiento que han tenido aplicaciones para la salud en adultos. Estetrabajo podría ayudar a promover la salud calidad de vida de la población adulta y eliminar elsedentarismo mediante la prescripción de la actividad física para la salud.
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Un modelo de equilibrio general para la fijación de precios del gas natural en México
Directory of Open Access Journals (Sweden)
Dagobert L. Brito
2005-01-01
Full Text Available La Comisión Reguladora de Energía de México ha implantado una regla deen la ce ha cia atrás para unir el pre cio del gas na tu ral me xi ca no al pre cio delgas na tu ral de Te jas. Este en sa yo de mues tra que en una eco no mía abier ta,en la que los agen tes es co gen en tre el gas y otros com bus ti bles, y en la que lafun ción de den si dad que des cri be la dis tri bu ción de los agen tes a lo lar go delgasoducto puede tener intervalos vacíos y puntos de acumulación, la regla deen la ce ha cia atrás es óp ti ma en el sen ti do de Pa re to.
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Feasibility of small animal cranial irradiation with the microRT system
International Nuclear Information System (INIS)
Kiehl, Erich L.; Stojadinovic, Strahinja; Malinowski, Kathleen T.; Limbrick, David; Jost, Sarah C.; Garbow, Joel R.; Rubin, Joshua B.; Deasy, Joseph O.; Khullar, Divya; Izaguirre, Enrique W.; Parikh, Parag J.; Low, Daniel A.; Hope, Andrew J.
2008-01-01
Purpose: To develop and validate methods for small-animal CNS radiotherapy using the microRT system. Materials and Methods: A custom head immobilizer was designed and built to integrate with a pre-existing microRT animal couch. The Delrin couch-immobilizer assembly, compatible with multiple imaging modalities (CT, microCT, microMR, microPET, microSPECT, optical), was first imaged via CT in order to verify the safety and reproducibility of the immobilization method. Once verified, the subject animals were CT-scanned while positioned within the couch-immobilizer assembly for treatment planning purposes. The resultant images were then imported into CERR, an in-house-developed research treatment planning system, and registered to the microRTP treatment planning space using rigid registration. The targeted brain was then contoured and conformal radiotherapy plans were constructed for two separate studies: (1) a whole-brain irradiation comprised of two lateral beams at the 90 degree sign and 270 degree sign microRT treatment positions and (2) a hemispheric (left-brain) irradiation comprised of a single A-P vertex beam at the 0 degree sign microRT treatment position. During treatment, subject animals (n=48) were positioned to the CERR-generated treatment coordinates using the three-axis microRT motor positioning system and were irradiated using a clinical Ir-192 high-dose-rate remote after-loading system. The radiation treatment course consisted of 5 Gy fractions, 3 days per week. 90% of the subjects received a total dose of 30 Gy and 10% received a dose of 60 Gy. Results: Image analysis verified the safety and reproducibility of the immobilizer. CT scans generated from repeated reloading and repositioning of the same subject animal in the couch-immobilizer assembly were fused to a baseline CT. The resultant analysis revealed a 0.09 mm average, center-of-mass translocation and negligible volumetric error in the contoured, murine brain. The experimental use of the head
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Características de calidad y digestibilidad in vitro del almidón agrio de yuca (Manihot esculenta producido en Costa Rica
Directory of Open Access Journals (Sweden)
Ana Ruth Bonilla Leiva
2012-01-01
Full Text Available Se evaluaron las características físicas, químicas y microbiológicas del almidón agrio de yuca producido a partir de las variedades de yuca "Valencia" y "Brasileña". El almidón agrio se obtuvo luego de un proceso de fermentación natural por 30 días y una posterior deshidratación mediante dos métodos: exposición directa al sol y en un secador solar. El proceso de fermentación para los almidones de ambas variedades se caracterizó por un aumento de la acidez titulable. Las propiedades físicas y químicas del almidón agrio de las dos variedades secados bajo los dos métodos se compararon con el almidón nativo. Se obtuvo una capacidad de expansión mayor (p 0,05. Se observó una forma esférica y truncada, así como la presencia del hilum en los gránulos de todos los almidones estudiados, sin embargo, en los almidones agrios presentó una apariencia con perforaciones, hendiduras y fisuras. Con respecto al tamaño de los gránulos se obtuvo un intervalo de 12,8 - 14,0 μm para el almidón nativo, mientras que el almidón agrio presentó valores de 11,3 - 11,6 μm. Se midió la digestibilidad in vitro del producto horneado obtenido de la prueba de expansión del almidón agrio variedad „Valencia‟ y deshidratado en secador solar, por considerarse el más factible de procesar industrialmente. Se obtuvo 44 g/100 g de almidón lentamente digerible.
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Características de calidad y digestibilidad in vitro del almidón agrio de yuca (Manihot esculenta producido en Costa Rica
Directory of Open Access Journals (Sweden)
Pedro Vargas Aguilar
2012-06-01
Full Text Available Se evaluaron las características físicas, químicas y microbiológicas del almidón agrio de yuca producido a partir de las variedades de yuca ‘Valencia’ y ‘Brasileña’. El almidón agrio se obtuvo luego de un proceso de fermentación natural por 30 días y una posterior deshidratación mediante dos métodos: exposición directa al sol y en un secador solar. El proceso de fermentación para los almidones de ambas variedades se caracterizó por un aumento de la acidez titulable. Las propiedades físicas y química del almidón agrio de las dos variedades secados bajo los dos métodos se compararon con el almidón nativo. Se obtuvo una capacidad de expansión mayor (p 0,05. Se observó una forma esférica y truncada, así como la presencia del hilum en los gránulos de todos los almidones estudiados, sin embargo, en los almidones agrios presentó una apariencia con perforaciones, hendiduras y fisuras. Con respecto al tamaño de los gránulos se obtuvo un intervalo de 12,8 - 14,0 µm para el almidón nativo, mientras que el almidón agrio presentó valores de 11,3 - 11,6 µm. Se midió la digestibilidad in vitro del producto horneado obtenido de la prueba de expansión del almidón agrio variedad ‘Valencia’ y deshidratado en secador solar, por considerarse el más factible de procesar industrialmente. Se obtuvo 44 g/100 g de almidón lentamente digerible.
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Diagnóstico de virus respiratorios utilizando un sistema automatizado de PCR múltiples (FilmArray y su comparación con métodos convencionales
Directory of Open Access Journals (Sweden)
Débora N Marcone
2015-03-01
Full Text Available Las infecciones respiratorias agudas producen una importante morbimortalidad y comúnmente son causadas por virus. En Argentina, los programas de vigilancia epidemiológica se basan en la detección de antígenos virales por inmunofluorescencia (IF, aunque es bien conocido que los métodos moleculares son más sensibles. El panel respiratorio (PR FilmArray (PR-FilmArray es un equipo comercial automatizado de PCR múltiples que detecta 17 virus respiratorios y 3 bacterias, en un sistema cerrado que requiere 5 min de procesamiento y una 1 h de instrumentación. Se evaluó un total de 315 muestras respiratorias de niños menores de 6 años con infecciones respiratorias agudas por IF para 8 virus respiratorios y por RT-PCR para rinovirus. Posteriormente, estas muestras se estudiaron con el PR-FilmArray. La frecuencia de positividad al considerar los 9 virus estudiados por IF y RT-PCR fue del 75 %; por PR-FilmArray fue del 92 %. El porcentaje de acuerdo positivo entre ambas metodologías fue del 70,5 % y el de acuerdo negativo fue del 99,6 % (intervalo de confianza 95 %: 65,5-75,1 y 99,2-99,8, respectivamente. El PR-FilmArray permitió obtener un mayor diagnóstico positivo (97 % y detectó otros virus, como los coronavirus NL63, 229E, OC43 y HKU1 (10 % y los bocavirus (18 %. Además, permitió identificar coinfecciones múltiples (39 % con 2, 3, 4 y hasta 5 virus. Actualmente, la IF continúa siendo el método más utilizado en los países latinoamericanos para el diagnóstico de virus respiratorios por su bajo costo, por su capacidad para procesar un alto número de muestras simultáneamente y porque los resultados de los virus más frecuentes están disponibles en 5 h. Sin embargo, la futura incorporación de métodos moleculares aumentaría notablemente la capacidad diagnóstica.
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Association Between RT-Induced Changes in Lung Tissue Density and Global Lung Function
International Nuclear Information System (INIS)
Ma Jinli; Zhang Junan; Zhou Sumin; Hubbs, Jessica L.; Foltz, Rodney J.; Hollis, Donna R.; Light, Kim L.; Wong, Terence Z.; Kelsey, Christopher R.; Marks, Lawrence B.
2009-01-01
Purpose: To assess the association between radiotherapy (RT)-induced changes in computed tomography (CT)-defined lung tissue density and pulmonary function tests (PFTs). Methods and Materials: Patients undergoing incidental partial lung RT were prospectively assessed for global (PFTs) and regional (CT and single photon emission CT [SPECT]) lung function before and, serially, after RT. The percent reductions in the PFT and the average changes in lung density were compared (Pearson correlations) in the overall group and subgroups stratified according to various clinical factors. Comparisons were also made between the CT- and SPECT-based computations using the Mann-Whitney U test. Results: Between 1991 and 2004, 343 patients were enrolled in this study. Of these, 111 patients had a total of 203 concurrent post-RT evaluations of changes in lung density and PFTs available for the analyses, and 81 patients had a total of 141 concurrent post-RT SPECT images. The average increases in lung density were related to the percent reductions in the PFTs, albeit with modest correlation coefficients (range, 0.20-0.43). The analyses also indicated that the association between lung density and PFT changes is essentially equivalent to the corresponding association with SPECT-defined lung perfusion. Conclusion: We found a weak quantitative association between the degree of increase in lung density as defined by CT and the percent reduction in the PFTs.
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Modified Polyadenylation-Based RT-qPCR Increases Selectivity of Amplification of 3′-MicroRNA Isoforms
Directory of Open Access Journals (Sweden)
Charlotte Nejad
2018-01-01
Full Text Available MicroRNA (miRNA detection by reverse transcription (RT quantitative real-time PCR (RT-qPCR is the most popular method currently used to measure miRNA expression. Although the majority of miRNA families are constituted of several 3′-end length variants (“isomiRs”, little attention has been paid to their differential detection by RT-qPCR. However, recent evidence indicates that 3′-end miRNA isoforms can exhibit 3′-length specific regulatory functions, underlining the need to develop strategies to differentiate 3′-isomiRs by RT-qPCR approaches. We demonstrate here that polyadenylation-based RT-qPCR strategies targeted to 20–21 nt isoforms amplify entire miRNA families, but that primers targeted to >22 nt isoforms were specific to >21 nt isoforms. Based on this observation, we developed a simple method to increase selectivity of polyadenylation-based RT-qPCR assays toward shorter isoforms, and demonstrate its capacity to help distinguish short RNAs from longer ones, using synthetic RNAs and biological samples with altered isomiR stoichiometry. Our approach can be adapted to many polyadenylation-based RT-qPCR technologies already exiting, providing a convenient way to distinguish long and short 3′-isomiRs.
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SU-E-J-42: Customized Deformable Image Registration Using Open-Source Software SlicerRT
Energy Technology Data Exchange (ETDEWEB)
Gaitan, J Cifuentes; Chin, L; Pignol, J [Sunnybrook Health Sciences Centre, Toronto, Ontario (Canada); Kirby, N; Pouliot, J [UC San Francisco, San Francisco, CA (United States); Lasso, A; Pinter, C; Fichtinger, G [Queen' s University, Kingston, Ontario (Canada)
2014-06-01
Purpose: SlicerRT is a flexible platform that allows the user to incorporate the necessary images registration and processing tools to improve clinical workflow. This work validates the accuracy and the versatility of the deformable image registration algorithm of the free open-source software SlicerRT using a deformable physical pelvic phantom versus available commercial image fusion algorithms. Methods: Optical camera images of nonradiopaque markers implanted in an anatomical pelvic phantom were used to measure the ground-truth deformation and evaluate the theoretical deformations for several DIR algorithms. To perform the registration, full and empty bladder computed tomography (CT) images of the phantom were obtained and used as fixed and moving images, respectively. The DIR module, found in SlicerRT, used a B-spline deformable image registration with multiple optimization parameters that allowed customization of the registration including a regularization term that controlled the amount of local voxel displacement. The virtual deformation field at the center of the phantom was obtained and compared to the experimental ground-truth values. The parameters of SlicerRT were then varied to improve spatial accuracy. To quantify image similarity, the mean absolute difference (MAD) parameter using Hounsfield units was calculated. In addition, the Dice coefficient of the contoured rectum was evaluated to validate the strength of the algorithm to transfer anatomical contours. Results: Overall, SlicerRT achieved one of the lowest MAD values across the algorithm spectrum, but slightly smaller mean spatial errors in comparison to MIM software (MIM). On the other hand, SlicerRT created higher mean spatial errors than Velocity Medical Solutions (VEL), although obtaining an improvement on the DICE to 0.91. The large spatial errors were attributed to the poor contrast in the prostate bladder interface of the phantom. Conclusion: Based phantom validation, SlicerRT is capable of
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Influência do intervalo de tempo entre as sessões de alongamento no ganho de flexibilidade dos isquiotibiais Influence of the time interval between stretching sessions on increased hamstring flexibility
Directory of Open Access Journals (Sweden)
Zenewton André da Silva Gama
2009-04-01
Full Text Available CONTEXTO: A otimização do treino de flexibilidade está relacionada com o uso de parâmetros adequados de alongamento. Entretanto, o intervalo de tempo adequado entre as sessões de alongamento tem sido pouco investigado. OBJETIVO: Verificar se a variação no intervalo de tempo entre sessões de alongamento influencia no ganho de flexibilidade. MÉTODOS: 28 mulheres, com idade de 22,5 ± 1,8 anos, foram distribuídas aleatoriamente em três grupos. Aplicaram-se 10 sessões de alongamento nos isquiotibiais do membro direito. O grupo 0X (n = 8 foi o controle e não recebeu alongamento. O grupo 3X (n = 10 alongou três vezes por semana (intervalo de 48 horas e o grupo 5X (n = 10, cinco vezes (intervalo de 24 horas. Aplicaram-se 10 sessões de alongamento (sustentar-relaxar nos isquiotibiais direitos. As medidas foram tomadas por análise fotométrica no programa AutoCad® 2000. A análise estatística foi realizada com ANOVA e teste post hoc de Newman-Keuls adotando um p-valor referencial de 0,05. RESULTADOS: Após 10 sessões, identificou-se aumento da flexibilidade nos grupos experimentais, porém sem diferença entre estes. O grupo 3X aumentou significativamente a partir do 10º dia do programa (quinta sessão e o grupo 5X, a partir do terceiro (terceira sessão. CONCLUSÕES: O alongamento aumenta a flexibilidade dos isquiotibiais, independente do tempo entre as sessões (24 ou 48 horas; e a variável tempo não influencia o ganho de flexibilidade total. Porém, com cinco sessões semanais, ganha-se flexibilidade mais rapidamente. Isso sugere que o ganho de flexibilidade é sessão-dependente.CONTEXT: The optimization of flexibility training is related to the use of ideal stretching parameters. However, the time interval between sessions has been little investigated. OBJECTIVE: To verify if the variation in time interval between stretching sessions influences in flexibility gain. METHODOLOGY: Twenty-eight women, average age of 22.5 ± 1
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Table 1. Primer sequences used for real-time qRT-PCR analysis of ...
Indian Academy of Sciences (India)
User
TGTCCCAGTAAACCGCTC. GAATCCAGCACGATACCAGT. Figure 1. Expression analysis of candidate CsActin and CsFbox genes by qRT-PCR in response to 4°C treatment. The y-axis indicates Cq values, and error bars represent standard deviations of the mean values of four replicates. Rt, roots; St, stems; Le, leaves; Fl ...
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Angioplastia del tronco de la arteria coronaria izquierda no protegido en pacientes con alto riesgo quirúrgico
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Victor Mauro
2008-01-01
Full Text Available IntroducciónEl tratamiento de elección de la enfermedad del tronco de la coronaria izquierda (TCI es la cirugía de revascularización miocárdica (CRM. Un número creciente de pacientes presenta comorbilidades y/o inestabilidad clínica que condicionan un alto riesgo quirúrgico.ObjetivosEvaluar los resultados de la angioplastia (ATC del TCI no protegido en pacientes con alto riesgo para CRM (EUROSCORE = 6.Material y métodosDe 59 pacientes con ATC de TCI no protegido se excluyeron 8 con infarto agudo de miocardio (IAM en shock cardiogénico y 12 sin características de alto riesgo; de los restantes pacientes de alto riesgo fueron objeto de este estudio los 32 tratados con stents convencionales.Se comparó la mortalidad hospitalaria predicha por EUROSCORE logístico con la observada, así como la incidencia de complicaciones mayores y su evolución alejada.ResultadosLa mediana de edad fue de 76,5 años, el 41% tenía 80 años o más, el 22% eran mujeres, el 28% diabéticos, el 56% tenía disfunción ventricular moderada a grave, el 31% insuficiencia renal crónica, el 50% vasculopatía periférica, el 53% angina refractaria, el 22% IAM reciente, el 28% procedimientos de emergencia y la mediana de EUROSCORE fue de 10,5 puntos.El 41% de los pacientes presentaban compromiso del TCI distal. El éxito angiográfico fue del 94%. Se utilizaron inhibidores IIb/IIIa en el 47%, cutting balloon en el 28%, Rotablator® en el 3% y balón de contrapulsación en el 31%. En todos se implantó un stent y en el 50% se trataron otras obstrucciones.La mortalidad hospitalaria fue del 3,1% (intervalo de confianza del 95% 0,2%-14,5%, p = 0,003, en tanto que la predicha era del 23,8%. Ningún paciente presentó déficit neurológico, IAM transmural ni requirió diálisis. Un paciente debió ser sometido a CRM electiva por fracaso del procedimiento.La mediana de seguimiento fue de 15,5 meses, período en el que se registraron 6 muertes (2 cardiovasculares y 4
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Synthesiomyia nudiseta (Diptera: Muscidae) y su importancia en Entomología Forense e investigación aplicada
OpenAIRE
Ivorra Cazorla, Tania
2016-01-01
Synthesiomyia nudiseta (van der Wulp, 1883) es un múscido de origen tropical y subtropical, siendo la única especie conocida dentro del género Synthesiomyia Brauer & Bergenstamm, 1893. Se trata de una especie con gran importancia forense para la estimación del intervalo postmortem, en diferentes zonas del mundo (p. ej.: Costa Rica, Estados Unidos, India, Malasia, Tailandia). Su presencia en España y Europa es relativamente reciente y en los últimos años se ha citado con cierta frecuencia en a...
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25 kaadrit sekundis / Liis Auväärt
Index Scriptorium Estoniae
Auväärt, Liis
2009-01-01
Nukufilmis käib töö filmiga "Lisa Limone & Maroc Orange", režissöör Mait Laas, animaatorid Triin Sarapik-Kivi, Märt Kivi, operaator Ragnar Neljandi, nukumeistrid Heigo Eeriksoo, Taivo Müürsep, Ene Mellow ja Kreeta Käeri. Dekoratsioonimeistrid Mait Erik ja Roman Kuznetsov
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A novel and highly sensitive real-time nested RT-PCR assay in a single closed tube for detection of enterovirus.
Science.gov (United States)
Shen, Xin-Xin; Qiu, Fang-Zhou; Zhao, Huai-Long; Yang, Meng-Jie; Hong, Liu; Xu, Song-Tao; Zhou, Shuai-Feng; Li, Gui-Xia; Feng, Zhi-Shan; Ma, Xue-Jun
2018-03-01
The sensitivity of qRT-PCR assay is not adequate for the detection of the samples with lower viral load, particularly in the cerebrospinal fluid (CSF) of patients. Here, we present the development of a highly sensitive real-time nested RT-PCR (RTN RT-PCR) assay in a single closed tube for detection of human enterovirus (HEV). The clinical performance of both RTN RT-PCR and qRT-PCR was also tested and compared using 140 CSF and fecal specimens. The sensitivities of RTN RT-PCR assay for EV71, Coxsackievirus A (CVA)16, CVA6 and CVA10 achieved 10 -8 dilution with a corresponding Ct value of 38.20, 36.45, 36.75, and 36.45, respectively, which is equal to traditional two-step nested RT-PCR assay and approximately 2-10-fold lower than that of qRT-PCR assay. The specificity of RTN RT-PCR assay was extensively analyzed insilico and subsequently verified using the reference isolates and clinical samples. Sixteen qRT-PCR-negative samples were detected by RTN RT-PCR and a variety of enterovirus serotypes was identified by sequencing of inner PCR products. We conclude RTN RT-PCR is more sensitive than qRT-PCR for the detection of HEV in clinical samples. Copyright © 2017 Elsevier Inc. All rights reserved.
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Reproducción del Cucarachero Común (Troglodytes aëdon, Aves, Troglodytidae en el Valle del Cauca Reproducción del Cucarachero Común (Troglodytes aëdon, Aves, Troglodytidae en el Valle del Cauca
Directory of Open Access Journals (Sweden)
Alvarez López Humberto
1984-09-01
.8 fledgelings per brood, a 90.2% nesting success. Breeding activity was recorded in every month of the year except March. Peaks of breeding in april and november-december coincided with rainfall peaks.EI presente trabajo describe la reproducción de Troglodytes aëdon (Aves:Troglodytidae en el Valle del Cauca (3°32' 20" N y 76° 31' 57" W, 976 m, Colombia, con base en observaciones de 24 nidos y de 44 individuos (11 adultos, 33 juveniles anillados para su reconocimiento individual. Los machos mantuvieron territorios permanentes de propósito múltiple cuyo tamafio promedio fluctuó entre 0.12 y 0.28 Ha. EI vínculo sexual monogámico se conservó a 10 largo de varias nidadas sucesivas y todos los cambios en la composición de las parejas siguieron a la desaparición de uno de los miembros.T. aëdon anida generalmente en cavidades y la mayoría de los nidos del presente estudio fueron construidos en nidales artificiales. Macho y hembra participaron en el proceso de la construcción, siendo notablemente mayor elaporte de la hembra en la etapa final. La construcción del nido ocupó entre 8 y 37 días, Los huevos fueron puestos a intervalos de aproximadamente 24hr en las primeras horas de la manana. Sus dimensiones y peso, en promedio, fueron respectivamente 18.5 x 13.8 mm y 1.8 g. EI tamaño de la nidada fluctuó entre 2 y 4 huevos, con un promedio de 3.2. El máximo número de nidadas para una hembra fue de 7 (20 huevos en un período de 17 meses y con un intervalo mínimo de 48 días entre posturas. Unicamente las hembras incubaron y dedicaron a este proceso entre 65 y 88% de su tiempo diurno. El promedio de duración para el período de incubación fue de 15 días y concluyó con eclosiones tanto seriadas como simultáneas, Durante los primeros siete días la hembra dedico el 37% de su tiempo diurno a cubrir los polluelos.Ambos padres alimentaron a los polluelos, siendo significativamente mayor el aporte de la hernbra. El promedio horario de visitas fue de 2.8 por polluelo
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Estimación de la masa corporal en géneros del Orden Notoungulata
Directory of Open Access Journals (Sweden)
Elissamburu, A.
2012-06-01
ecuaciones predictivas para tal fin, respecto a cada taxón en particular, a cada familia y al Orden en general. Se incluyen 535 ejemplares fósiles correspondientes a 50 géneros del orden. La masa corporal de cada género se estima sobre la base de 24 ecuaciones alométricas construidas en mamíferos vivientes, basadas en 19 medidas del esqueleto apendicular y tres medidas dentarias tomadas con calibre digital. El intervalo de masa corporal dado por los valores estimados para el orden se encuentra entre 0,57 kg en Archaeophylus (Interatheriidae y 3797 Kg en Mixotoxodon (Toxodontidae, lo que refleja la gran diversidad de tamaños corporales que caracteriza al grupo. Los menores tamaños se dan en las familias Notostylopidae, Oldfieldthomasiidae, Archaeopithecidae, Interatheriidae, Archaeohyracidae y Hegetotheriidae; tamaños intermedios en los Notohippidae, Isotemnidae y Mesotheriidae y tamaños grandes en Homalodotheriidae. En los Leontiniidae y Toxodontidae los tamaños van de intermedios a grandes, siendo esta última la de mayor variación de masa y con los taxones de mayor tamaño. En comparación con los ungulados holárticos, los notoungulados presentan un intervalo más amplio y variable de masa corporal hacia las formas de menor tamaño y con mayor variedad funcional en las extremidades.
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PREVALENCIA DE LA DEFICIENCIA DE HIERRO EN DONANTES DE SANGRE. REVISIÓN BIBLIOGRÁFICA DEL PERÍODO 2001- 2011
Directory of Open Access Journals (Sweden)
Carmen Yulieth Mantilla-Gutiérrez
2012-01-01
Full Text Available Fundamentos: La donación de sangre se asocia con disminución de las reservas de hierro de los donantes, lo que puede afectar el desarrollo de sus funciones fisiológicas y al estado general de salud. Estudios previos reportan una gran divergencia en la prevalencia de deficiencia de hierro en dicha población (1% a 62%. El objetivo del trabajo es determinar la prevalencia de deficiencia de hierro en donantes de sangre a partir de una revisión sistemática de la literatura. Métodos: Búsqueda exhaustiva y reproducible de la literatura en 7 bases de datos con base en un protocolo de búsqueda de estudios en 4 idiomas y publicados entre el 2001 y el 2011 con criterios de inclusión, exclusión y extracción de información. El análisis se realizó con base en el cálculo de la prevalencia global y específica por sexo y número de donaciones, con sus respectivos intervalos de confianza, utilizando los programas Excel y Epidat 3.0. Resultados: Se obtuvo una población total de 16.979 donantes, 5.096 de repetición, con una proporción de hombres del 59%. Se halló una prevalencia de deficiencia de hierro del 13% (IC 95% 12,4-13,4, con una rango entre 1% y 62%, siendo estadísticamente significativa la mayor frecuencia en mujeres (19,56% IC 95%: 18,59 - 20,53 y donantes repetidores (20,36% IC 95%: 19,25 - 21,48. Conclusión: Se obtuvo una prevalencia de deficiencia de hierro en donantes de sangre por encima de grupos de mayor riesgo como los niños, siendo mayor en donantes del sexo femenino y de repetición.
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Intervalos de integración unificados para la caracterización estructural de petróleos, carbones o sus fracciones por rmn 1h y rmn 13c
OpenAIRE
Eliseo, Avella; Fierro, Ricardo
2010-01-01
Con base en la revisión de publicaciones, hechas entre 1972 y 2006, se evidenció que hay imprecisiones en los límites de los intervalos de integración que los autores asignan a las señales en resonancia magnética nuclear (RMN) para hacer la caracterización estructural de petróleos, carbones o sus fracciones derivadas, a partir de sus espectros de hidrógeno (RMN 1H) o de carbono (RMN 13C). En consecuencia, se determinaron límites unificados para la integración de los espectros RMN 1H y RMN 13C...
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CYCLIC VARIATIONS OF ORBITAL PERIOD AND LONG-TERM LUMINOSITY IN CLOSE BINARY RT ANDROMEDAE
International Nuclear Information System (INIS)
Manzoori, Davood
2009-01-01
Solutions of standard VR light curves for the eclipsing binary RT And were obtained using the PHOEBE program (ver. 0.3a). Absolute parameters of the stellar components were then determined, enabling them to be positioned on the mass-luminosity diagram. Times of minima data ( O - C curve ) were analyzed using the method of Kalimeris et al. A cyclic variation in the orbital period and brightness, with timescales of about 11.89 and 12.50 yr were found, respectively. This is associated with a magnetic activity cycle modulating the orbital period of RT And via the Applegate mechanism. To check the consistency of the Applegate model, we have estimated some related parameters of the RT And system. The calculated parameters were in accordance with those estimated by Applegate for other similar systems, except B, the subsurface magnetic field of which shows a rather high value for RT And.
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Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix
Directory of Open Access Journals (Sweden)
Vázquez-Corzo Sara
2003-01-01
Full Text Available OBJETIVO: Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. MATERIAL Y MÉTODOS: Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELISA de captura. Se estimaron razones de momios e intervalos de confianza de 95% RESULTADOS: Anticuerpos anti-E7 se asociaron a mujeres con lesiones NIC III, mientras que anticuerpos anti-E4 y anti-Ras fueron más frecuentes en lesiones NIC I-II. Al evaluar el perfil de anticuerpos que presentaron las mujeres, encontramos que a anticuerpos contra dos proteínas predicen la existencia de una lesión NIC I-II, y b la presencia de tres anticuerpos predicen una lesión NIC III. CONCLUSIONES: La detección de anticuerpos séricos contra E4, E7 y Ras en combinación con otras técnicas de diagnóstico, podrían ser de utilidad para detectar oportunamente a mujeres con lesiones tempranas asociadas al Virus del Papiloma Humano y en riesgo de desarrollar cáncer.
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Arvo Pärt tänavu Holland-Festivali keskmes
Index Scriptorium Estoniae
2005-01-01
Klassikalise muusika festivalil "Holland-Festival" 1.-26. juun. Amsterdamis on aukülaliseks Arvo Pärt, kelle muusikat esitatakse 7.-10. ja 18. juunil Amsterdami Uues kirikus (Nieuwe Kerk), Muziekgebouw aan het Ij-is ja Concertgebouw's
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Pärt, Arvo: "Beatus". Statuit ei Dominus / Patric Wiklacz
Index Scriptorium Estoniae
Wiklacz, Patric
1997-01-01
Uuest heliplaadist "Pärt, Arvo: "Beatus". Statuit ei Dominus. Missa syllabica. Beatus Petronius. 7 Magnificat-Antiphonen. De profundis. Memento. Cantate Domino. Solfeggio. Estonian Philharmonic Chamber Choir, T. Kaljuste". Virgin Classics 545 276-2 (CD:167F)
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Evaluación físico - químico del dulce de leche saborizado en barra a diferentes temperaturas de conservación Empresa de Productos Lácteos Bayamo
Directory of Open Access Journals (Sweden)
Adriana Lucia Rodríguez Rodríguez
2015-09-01
Full Text Available La investigación se desarrolló con el propósito de diagnosticar las causas de afectaciones microbiológicas en el dulce de leche saborizado en barra antes de los 7 días de garantía, conservado a temperatura ambiente en los puntos de venta, deteriorando los parámetros de calidad del producto, por lo que se procedió a la realización de un diagnóstico en estos puntos donde se comercializa el surtido, relacionando los factores temperatura – humedad, así como entrevistas realizadas a clientes y trabajadores que allí laboran. En la industria también se realizó un diagnóstico que incluyó desde el estudio minucioso y análisis físicos-químicos, organolépticos y microbiológicos de todas las materias primas y materiales que se utilizan en la elaboración del dulce de leche, hasta el control de calidad del producto terminado expuesto a diferentes valores de temperatura y humedad relativa, con observación diaria de la muestras en vida estate y análisis realizados en intervalos de 7 días, determinando que la exposición del producto a temperatura ambiente propicia el desarrollo microbiano y que el menor valor de temperatura estudiado 18 0C, garantiza una durabilidad en el dulce de leche saborizado en barra de 30 días.
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Intervalo hídrico ótimo em um Latossolo vermelho cultivado em sistema semeadura direta por 25 anos
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Fernando Rodrigues Moreira
2014-02-01
Full Text Available O Intervalo Hídrico Ótimo (IHO integra atributos físicos do solo relacionados ao crescimento das culturas e corresponde ao intervalo entre os limites superior e inferior do conteúdo de água no solo, no qual são mínimas as limitações para o crescimento radicular. Em áreas agrícolas, o manejo do solo pode alterar sua estrutura, de forma que, principalmente em razão da compactação, a densidade do solo (Ds poderá ficar fora desses limites em que as condições são ideais para o crescimento das plantas. O objetivo deste trabalho foi utilizar o IHO para avaliação da qualidade física do solo e identificação de áreas com restrição para crescimento de plantas, visando ao manejo localizado. O estudo foi realizado em um Latossolo Vermelho argiloso sob sistema semeadura direta (SSD com cultivos sucessivos de grãos por 25 anos em Campinas, SP. Foram coletadas amostras volumétricas de solo, nas camadas de 0,00-0,10, 0,10-0,20 e 0,20-0,30 m, destinadas à obtenção dos seguintes atributos: Ds, curva de resistência à penetração, curva de retenção de água e porosidade do solo para determinação do IHO e da densidade crítica do solo (Dsc. Dados de variabilidade e dependência espacial da Ds foram analisados por semivariogramas para elaboração de mapas desse atributo. O IHO diminuiu em profundidade e foi limitado na parte superior pela umidade na capacidade de campo e na parte inferior pela resistência à penetração, nas três profundidades avaliadas. A Dsc foi de 1,42 Mg m-3 para a camada de 0,00-0,10 m e de 1,39 e 1,41 Mg m-3, respectivamente, para as camadas de 0,10-0,20 e 0,20-0,30 m. Verificou-se que a Ds foi maior que a Dsc nas camadas de 0,00-0,10 e 0,10-0,20 m, em porções localizadas no terço inferior do terreno, indicando condição crítica para o crescimento das plantas. A utilização do IHO, associado a mapas de variabilidade espacial da Ds, para determinação de pontos em que ela é maior que a Dsc
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Pärt: Chamber and Orchestral Works / Robert Cowan
Index Scriptorium Estoniae
Cowan, Robert
1994-01-01
Uuest heliplaadist "Pärt: Chamber and Orchestral Works. Bournemouth Sinfonietta, Richard Studt. EMI Eminence CD CD-EMX 2221; Fratres; Cantus in memory of Benjamin Britten; Summa; Spiegel im Spiegel; Festina lente; Tabula Rasa; Fratres - selected comparison: Bachmann, Kibonoff (12/94)(CATA) 09026 61824-2. Kremer, Jarrett (ECM) 817 764-2
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Domesticación animal y primeras prácticas ganaderas en el noreste peninsular (5500-4500 cal BC). Integración de los análisis bioquímicos en arqueozoología
OpenAIRE
Navarrete Belda, Vanessa
2017-01-01
En el presente trabajo de tesis doctoral se aborda la problemática de la dinámica del proceso de domesticación animal y las prácticas ganaderas iniciales en el extremo más occidental del área mediterránea a lo largo del intervalo temporal que abarca entre el 5.500-4.700 cal ANE. El objetivo principal de la tesis se aborda a partir del estudio de una muestra significativa de yacimientos neolíticos situados en el noreste de la Península Ibérica. Se ha evaluado con esta tesis doctoral los pr...
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Impacto clínico y económico de las intervenciones farmacéuticas
Directory of Open Access Journals (Sweden)
Gonzalo Rodríguez Torné
2011-03-01
Full Text Available Se realizó un estudio retrospectivo durante el periodo comprendido entre noviembre del 2008 y abril del 2009 a partir del sistema de distribución de medicamentos en dosis unitarias, con el objetivo de documentar el valor de la intervención farmacéutica, tanto en datos descriptivos como en datos económicos y de eficiencia, mediante el empleo de un programa de equivalentes terapéuticos. Las intervenciones farmacéuticas se agruparon en dosis/intervalo, sustitución, vía de administración, interacciones, alergias y otros. En cuanto al impacto económico, los valores de precios se obtuvieron a partir de los precios de compra de los medicamentos y del catálogo de especialidades farmacéuticas. Se realizaron un total de 297 intervenciones repartidas en 50 dosis/intervalo, 174 sustituciones, 34 de vía, 25 de interacciones, 3 de alergia y 11 en el apartado de otros. El ahorro real calculado supuso 18 146,39 euros. El registro de las intervenciones se valora como beneficioso para la detección de problemas prioritarios y áreas de actuación, además de constituir una herramienta útil para poder demostrar el coste/efectividad de las acciones.
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Thoracic radiation therapy (RT) before autologous bone marrow transplantation (ABMT) in relapsed or refractory Hodgkin's disease (HD)
International Nuclear Information System (INIS)
Tsang, Richard W.; Gospodarowicz, Mary K.; Sutcliffe, Simon B.; Crump, Michael; Keating, Armand
1997-01-01
Purpose: To assess the relationship between peri-ABMT RT and treatment-related mortality in patients receiving high-dose chemotherapy (HDCT) and ABMT for recurrent/refractory HD. Materials and Methods: Between 12/1986 and 12/1992, 90 patients (pts) with HD were treated with HDCT (etoposide 60 mg/kg, melphalan 160 mg/m 2 ) +ABMT in Toronto. Fifty-nine of these had all or part of their treatment (RT and/or CT) at the PMH, and they form the basis of this report. HDCT + ABMT was performed for refractory (11 pts) or relapsed (48 pts) HD. The median age at ABMT was 29 (range 16-47), F:M ratio was 1:1.2. Salvage chemotherapy consisted of DHAP (36), mini-BEAM (7), both (8), other chemotherapy (5), and no chemotherapy (3). RT have been incorporated in the salvage treatment with the intent to achieve complete disease control of active disease at relapse or disease progression. RT was given pre-ABMT in 33 pts (3 mantle, 8 mantle with lung, 7 modified mantle excluding axillae, 4 involved field, 1 mantle with TBI, 1 TBI, and 9 non-thoracic), and post-ABMT in 4 pts (2 mantle, 2 modified mantle). Planned RT tumor dose was 35 Gy over 4 weeks (median 35 Gy, range 25-40 Gy), lower doses were used for lung RT (15-17.5 Gy). Treatment-related (TR) mortality was defined as any death occurring within 100 days of ABMT. Autopsies were performed for all patients with TR deaths. Results: We observed 36 deaths, and 10 of these were treatment-related (TR) deaths. With an average follow-up of 4.9 years (range 1.2-7.4 years), the actuarial overall survival was 52% ± 13% at 3 years, and 41% ± 14% at 5 years. Among the 24 patients who received thoracic RT before ABMT, there were 8 TR deaths, with 3 solely attributable to radiation pneumonitis. The remaining 5 TR deaths all had respiratory failure as a major medical problem with complicating sepsis and pancytopenia. The interval from RT to ABMT was shorter for 8 patients dying of TR death (mean 37 days; range 0-103 days), than for the 16 pts with
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Detection and Analysis of Circular RNAs by RT-PCR.
Science.gov (United States)
Panda, Amaresh C; Gorospe, Myriam
2018-03-20
Gene expression in eukaryotic cells is tightly regulated at the transcriptional and posttranscriptional levels. Posttranscriptional processes, including pre-mRNA splicing, mRNA export, mRNA turnover, and mRNA translation, are controlled by RNA-binding proteins (RBPs) and noncoding (nc)RNAs. The vast family of ncRNAs comprises diverse regulatory RNAs, such as microRNAs and long noncoding (lnc)RNAs, but also the poorly explored class of circular (circ)RNAs. Although first discovered more than three decades ago by electron microscopy, only the advent of high-throughput RNA-sequencing (RNA-seq) and the development of innovative bioinformatic pipelines have begun to allow the systematic identification of circRNAs (Szabo and Salzman, 2016; Panda et al ., 2017b; Panda et al ., 2017c). However, the validation of true circRNAs identified by RNA sequencing requires other molecular biology techniques including reverse transcription (RT) followed by conventional or quantitative (q) polymerase chain reaction (PCR), and Northern blot analysis (Jeck and Sharpless, 2014). RT-qPCR analysis of circular RNAs using divergent primers has been widely used for the detection, validation, and sometimes quantification of circRNAs (Abdelmohsen et al ., 2015 and 2017; Panda et al ., 2017b). As detailed here, divergent primers designed to span the circRNA backsplice junction sequence can specifically amplify the circRNAs and not the counterpart linear RNA. In sum, RT-PCR analysis using divergent primers allows direct detection and quantification of circRNAs.
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The value of research : telling the R&T story
Science.gov (United States)
2009-07-01
The Federal Highway Administration (FHWA) plays a leadership role in shaping and executing a National Research and Technology (R&T) program. The agency also acts as a convener; collaborations with State, industry, and academic partners provide the fo...
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Metodiskā darba organizēšana un izvērtēšana skolā.
OpenAIRE
Jakubovska, Solvita
2010-01-01
Maģistra darbā „Metodiskā darba organizēšana un izvērtēšana skolā” aplūkoti jautājumi par metodiskā darba organizēšanu valsts, pašvaldības un skolas līmenī, kā arī metodisko komisiju darbības izvērtēšanu, balstoties uz skolu darba kvalitātes izvērtēšanu akreditācijas procesā. Maģistra darba mērķis ir izpētīt skolas metodiskā darba organizēšanas un izvērtēšanas iespējas, veidojot priekšlikumus darba uzlabošanai. Pētījuma ietveros tika izstrādāti skolas metodisko komisiju darbības izvērtēša...
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Novel HBV recombinants between genotypes B and C in 3'-terminal reverse transcriptase (RT) sequences are associated with enhanced viral DNA load, higher RT point mutation rates and place of birth among Chinese patients.
Science.gov (United States)
Liu, Baoming; Yang, Jing-Xian; Yan, Ling; Zhuang, Hui; Li, Tong
2018-01-01
As one of the major global public health concerns, hepatitis B virus (HBV) can be divided into at least eight genotypes, which may be related to disease severity and treatment response. We previously demonstrated that genotypes B and C HBV, with distinct geographical distribution in China, had divergent genotype-dependent amino acid polymorphisms and variations in reverse transcriptase (RT) gene region, a target of antiviral therapy using nucleos(t)ide analogues. Recently recombination between HBV genotypes B and C was reported to occur in the RT region. However, their frequency and clinical significance is poorly understood. Here full-length HBV RT sequences from 201 Chinese chronic hepatitis B (CHB) patients were amplified and sequenced, among which 31.34% (63/201) were genotype B whereas 68.66% (138/201) genotype C. Although no intergenotypic recombination was detected among C-genotype HBV, 38.10% (24/63) of B-genotype HBV had recombination with genotype C in the 3'-terminal RT sequences. The patients with B/C intergenotypic recombinants had significantly (Pdistribution feature in China. Our findings provide novel insight into the virological, clinical and epidemiological features of new HBV B/C intergenotypic recombinants at the 3' end of RT sequences among Chinese CHB patients. The highly complex genetic background of the novel recombinant HBV carrying new mutations affecting RT protein may contribute to an enhanced heterogeneity in treatment response or prognosis among CHB patients. Published by Elsevier B.V.
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Endogenous reverse transcriptase (RT) activity and Chromatin remodeling in normal and transformed cells and early embryos
International Nuclear Information System (INIS)
Spadafora, C.; Sciamanna, I.; Misteli, T.
2009-01-01
Endogenous Reverse Transcriptase (RT) is an enzyme encoded by two classes of genomic retro-elements: retro-transposons and endogenous retroviruses. Basal levels of RT are expressed in all non pathological, differentiated tissues while high RT expression levels characterize tumorigenic cells, germ cells and embryonic tissues. Preliminary studies carried out in our laboratory have shown that RT inhibition using pharmacological inhibitors (nevirapine and efavirenz, two drugs currently used in AIDS therapy) drastically reduces cell proliferation, promotes differentiation of tumorigenic cells in vitro, induces a reprogrammed gene expression and antagonizes tumor progression in nude mice inoculated with tumorigenic human cell lines, including melanoma, prostate and colon carcinoma and microcitoma
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Detecting Newcastle disease virus in combination of RT-PCR with red blood cell absorption
Directory of Open Access Journals (Sweden)
Liu Chengqian
2011-05-01
Full Text Available Abstract Reverse transcription-polymerase chain reaction (RT-PCR has limited sensitivity when treating complicated samples, such as feces, waste-water in farms, and nucleic acids, protein rich tissue samples, all the factors may interfere with the sensitivity of PCR test or generate false results. In this study, we developed a sensitive RT-PCR, combination of red blood cell adsorption, for detecting Newcastle disease virus (NDV. One pair of primers which was highly homologous to three NDV pathotypes was designed according to the consensus nucleocapsid protein (NP gene sequence. To eliminate the interfere of microbes and toxic substances, we concentrated and purified NDV from varied samples utilizing the ability of NDV binding red blood cells (RBCs. The RT-PCR coupled with red blood cell adsorption was much more sensitive in comparison with regular RT-PCR. The approach could also be used to detect other viruses with the property of hemagglutination, such as influenza viruses.
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Real-Time RT-PCR for the Detection of Lyssavirus Species
Directory of Open Access Journals (Sweden)
A. Deubelbeiss
2014-01-01
Full Text Available The causative agents of rabies are single-stranded, negative-sense RNA viruses in the genus Lyssavirus of Rhabdoviridae, consisting of twelve classified and three as yet unclassified species including classical rabies virus (RABV. Highly neurotropic RABV causes rapidly progressive encephalomyelitis with nearly invariable fatal outcome. Rapid and reliable diagnosis of rabies is highly relevant for public and veterinary health. Due to growing variety of the genus Lyssavirus observed, the development of suitable molecular assays for diagnosis and differentiation is challenging. This work focused on the establishment of a suitable real-time RT-PCR technique for rabies diagnosis as a complement to fluorescent antibody test and rabies tissue culture infection test as gold standard for diagnosis and confirmation. The real-time RT-PCR was adapted with the goal to detect the whole spectrum of lyssavirus species, for nine of which synthesized DNA fragments were used. For the detection of species, seven probes were developed. Serial dilutions of the rabies virus strain CVS-11 showed a 100-fold higher sensitivity of real-time PCR compared to heminested RT-PCR. Using a panel of thirty-one lyssaviruses representing four species, the suitability of the protocol could be shown. Phylogenetic analysis of the sequences obtained by heminested PCR allowed correct classification of all viruses used.
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Performance Evaluation of Irbene RT-16 Radio Telescope Receiving System
Directory of Open Access Journals (Sweden)
Bleiders M.
2017-12-01
Full Text Available In the present paper, recent measurement results of refurbished Irbene RT-16 radio telescope receiving system performance are presented. The aim of the research is to evaluate characteristics of RT-16, which will allow carrying out necessary amplitude calibration in both single dish and VLBI observations, to improve the performance of existing system as well as to monitor, control and compare performance if possible changes in the receiving system will occur in future. The evaluated receiving system is 16 m Cassegrain antenna equipped with a cryogenic receiver with frequency range from 4.5 to 8.8 GHz, which is divided into four sub-bands. Multiple calibration sessions have been carried out by observing stable astronomical sources with known flux density by using in-house made total power registration backend. First, pointing offset calibration has been carried out and pointing model coefficients calculated and applied. Then, amplitude calibration, namely antenna sensitivity, calibration diode equivalent flux density and gain curve measurements have been carried out by observing calibration sources at different antenna elevations at each of the receiver sub-bands. Beam patterns have also been evaluated at different frequency bands. As a whole, acquired data will serve as a reference point for comparison in future performance evaluation of RT-16.
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Detection of avian metapneumovirus subtypes in turkeys using RT-PCR.
Science.gov (United States)
Ongor, H; Karahan, M; Kalin, R; Bulut, H; Cetinkaya, B
2010-03-20
This study investigated the prevalence of avian metapneumovirus (aMPV) and the detection of molecular subtypes of field strains of the virus using RT-PCR in clinically healthy turkeys and those showing signs of respiratory disease. In the RT-PCR examination of 624 tracheal tissue samples collected from a local turkey abattoir, 2.9 per cent (18/624) of samples tested positive. In the examination of tracheal swab samples collected from flocks with respiratory problems, 18 of 20 samples tested positive. When the results were assessed at flock level, aMPV infection was detected in only one of the 23 clinically healthy turkey flocks, whereas all four flocks with respiratory problems were infected. Molecular typing using primers specific to the attachment glycoprotein (G) gene showed that all 36 positive samples belonged to subtype B. Partial sequence analysis of DNA samples showed 95 per cent homology between the field types and the reference strain aMPV subtype B. Whereas clinically healthy turkeys had been vaccinated with a subtype A virus vaccine, the flocks with respiratory problems had been vaccinated with a subtype B virus vaccine. Despite four blind passages of RT-PCR-positive samples on Vero and chicken embryo fibroblast cells, no cytopathic effect was detected by microscopic examination.
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Responsividade do serviço de enfermagem na visão do cliente Responsividad del servicio de enfermería en la visión del paciente Nursing care responsiveness from the client's view
Directory of Open Access Journals (Sweden)
Ana Vanessa Deffaccio Rodrigues
2012-12-01
Full Text Available A expectativa criada pelo indivíduo na hospitalização e a percepção que ele tem do cuidado que recebe quando hospitalizado são aspectos que definem seu grau de satisfação. Assim, surgiu o conceito responsividade no campo da avaliação em saúde. O objetivo deste estudo é mensurar a responsividade do serviço de enfermagem de uma unidade médico-cirúrgica de um hospital universitário público, na visão do cliente, por meio de um instrumento que aborda duas categorias: Expectativas e Percepções. Trata-se de pesquisa quantitativa que utilizou entrevistas. Para análise dos dados realizou-se o cálculo da taxa de satisfação relativa e do intervalo, o que possibilitou a classificação do serviço de enfermagem. Correlacionam-se as características socioeconômicas com as experiências prévias de internação dos entrevistados. Os resultados demonstraram que o serviço de enfermagem está com uma representação Adequada. Alguns fatores foram considerados responsáveis pelo fenômeno da alta satisfação, como viés de gratidão e de aquiescência.La expectativa creada por el individuo hospitalizado y su percepción del cuidado que recibe mientras está hospitalizado son aspectos que definen su grado de satisfacción. De ese modo surgió el concepto de responsividad en el campo de evaluación en salud. Este estudio objetivó mensurar la responsividad del servicio de enfermería de una unidad médico-quirúrgica de hospital universitario público, en la visión del paciente, mediante un instrumento que aborda dos categorías: Expectativas y Percepciones. Investigación cuantitativa que utilizó entrevistas. Datos analizados por cálculo de tasa de satisfacción relativa e intervalo, lo cual posibilitó la clasificación del servicio de enfermería. Se correlacionan las características socioeconómicas con las experiencias previas de internación de los entrevistados. Los resultados demuestran que el servicio de enfermería se
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Detection of respiratory bacterial pathogens causing atypical pneumonia by multiplex Lightmix® RT-PCR.
Science.gov (United States)
Wagner, Karoline; Springer, Burkard; Imkamp, Frank; Opota, Onya; Greub, Gilbert; Keller, Peter M
2018-04-01
Pneumonia is a severe infectious disease. In addition to common viruses and bacterial pathogens (e.g. Streptococcus pneumoniae), fastidious respiratory pathogens like Chlamydia pneumoniae, Mycoplasma pneumoniae and Legionella spp. can cause severe atypical pneumonia. They do not respond to penicillin derivatives, which may cause failure of antibiotic empirical therapy. The same applies for infections with B. pertussis and B. parapertussis, the cause of pertussis disease, that may present atypically and need to be treated with macrolides. Moreover, these fastidious bacteria are difficult to identify by culture or serology, and therefore often remain undetected. Thus, rapid and accurate identification of bacterial pathogens causing atypical pneumonia is crucial. We performed a retrospective method evaluation study to evaluate the diagnostic performance of the new, commercially available Lightmix ® multiplex RT-PCR assay that detects these fastidious bacterial pathogens causing atypical pneumonia. In this retrospective study, 368 clinical respiratory specimens, obtained from patients suffering from atypical pneumonia that have been tested negative for the presence of common agents of pneumonia by culture and viral PCR, were investigated. These clinical specimens have been previously characterized by singleplex RT-PCR assays in our diagnostic laboratory and were used to evaluate the diagnostic performance of the respiratory multiplex Lightmix ® RT-PCR. The multiplex RT-PCR displayed a limit of detection between 5 and 10 DNA copies for different in-panel organisms and showed identical performance characteristics with respect to specificity and sensitivity as in-house singleplex RT-PCRs for pathogen detection. The Lightmix ® multiplex RT-PCR assay represents a low-cost, time-saving and accurate diagnostic tool with high throughput potential. The time-to-result using an automated DNA extraction device for respiratory specimens followed by multiplex RT-PCR detection was
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Curva de referência da área do septo interventricular fetal pelo método STIC: estudo preliminar Curva de referencia del área del septo interventricular fetal por el método STIC: estudio preliminar Reference curve of the fetal ventricular septum area by the STIC method: preliminary study
Directory of Open Access Journals (Sweden)
Liliam Cristine Rolo
2011-05-01
reprodutíveis.FUNDAMENTO: La detección precoz de alteraciones septales, tales como la hipertrofia septal comúnmente presente en fetos de madres diabéticas, contribuiría a la reducción de las altas tasas de mortalidad infantil. OBJETIVO: Determinar intervalos de referencia para el área del septo interventricular fetal por medio de la ultrasonografía tridimensional (US3D utilizando el método STIC (Spatio-Temporal Image Correlation. MÉTODOS: Se realizó un estudio de corte transversal con 69 gestantes normales entre la 18ª y 33ª semanas de gestación. Se utilizó como referencia el plano de cuatro cámaras con la ROI (Región de Interés posicionada a partir de los ventrículos, siendo el área del septo delimitada de modo manual. Para evaluar la correlación del área del septo interventricular con la edad gestacional (EG, se construyeron diagramas de dispersión y se calculó el coeficiente de correlación de Pearson (r, siendo el ajuste realizado por el coeficiente de determinación (R². Fueron calculadas medias, medianas, desviaciones-estándar (de, valores máximo y mínimo. Para el cálculo de la reproductibilidad intraobservador, se utilizó el coeficiente de correlación intraclase (CCI. Se obtuvo la medida del espesor del septo interventricular y la misma fue correlacionada con la EG y el área septal obtenida por el modo renderizado en 52 pacientes utilizando el CCI. RESULTADOS: El área del septo interventricular fue altamente correlacionada con la edad gestacional (r = 0,81, y la media aumentó de 0,47 cm² en la 18ª a 2,42 cm² en la 33ª semana de gestación. La reproductibilidad intraobservador fue excelente con CCI = 0,994. No se observó correlación significativa entre la medida del septo interventricular y la EG (R² = 0,200, así como no hubo correlación con el área del septo obtenida por el modo renderizado con CCI = 0,150. CONCLUSIÓN: Intervalos de referencia para el área del septo interventricular entre la 18ª y 33ª semanas de
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The cost-effectiveness of routine radiation therapy (RT) following conservative surgery (CS) for early-stage breast cancer
International Nuclear Information System (INIS)
Hayman, James A.; Hillner, Bruce E.; Harris, Jay R.; Weeks, Jane C.
1996-01-01
Purpose: The routine use of RT following CS has been shown to decrease the rate of LR by at least 75%, but its ultimate impact on survival remains less clear. Given concerns about its toxicity, inconvenience, and cost, the need for routine RT has been questioned. These concerns, however, need to be balanced against the improved quality of life and reduced salvage costs associated with routine RT. We performed an incremental cost-utility analysis to evaluate whether, even in the assumed absence of a survival advantage, the use of RT in this situation would be considered cost-effective. Materials and Methods: A Markov decision analytic model was developed to examine the incremental impact of the addition of RT to CS on the relevant outcomes and costs from a societal perspective. Using a cycle length of one year, the analysis was performed on a theoretical cohort of 60 year old patients over a 10 year time horizon. To account for quality of life benefits, outcomes were measured in Quality Adjusted Life Years (QALYs). The LR, distant recurrence (DR), and overall survival rates used in the model were drawn principally from NSABP B-06. It was assumed for the baseline analysis that the addition of RT reduces the rate of LR by 75%, but has no impact on overall survival. Salvage for LR following CS and RT consisted of mastectomy and reconstructive surgery (MRS), while, following CS alone, it was assumed that equal numbers of patients would be salvaged by CS and RT and by MRS. The rate of DR was assumed to be constant with and without RT. The utilities of 97 patients with early-stage breast cancer treated with CS and RT were used to adjust the relevant local outcomes for quality of life, while expert judgement was used to estimate the utilities for the DR states. Baseline costs were determined using Medicare charges, ratios of costs to charges and RVUs, all at the Medical College of Virginia. When uncertainties existed, the model was biased against RT. Both benefits and
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A one-step multiplex RT-PCR assay for simultaneous detection of four viruses that infect peach.
Science.gov (United States)
Yu, Y; Zhao, Z; Jiang, D; Wu, Z; Li, S
2013-10-01
A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed to enable the simultaneous detection and differentiation of four viruses that infect peach, namely Apple chlorotic leaf spot virus (ACLSV), Cherry green ring mottle virus (CGRMV), Prunus necrotic ringspot virus (PNRSV) and Apricot pseudo-chlorotic leaf spot virus (APCLSV). In this study, four pairs of primers, one specific for each virus, were designed; the corresponding PCR products were 632, 439, 346 and 282 bp in length for ACLSV, CGRMV, PNRSV and APCLSV, respectively, and the fragments could be distinguished clearly by agarose gel electrophoresis. The sensitivity and specificity of the method were tested using individual RT-PCR and enzyme-linked immunosorbent assay (ELISA), and the identity of the RT-PCR amplification products was also confirmed by DNA sequencing. The results of RT-PCR and ELISA, along with batch detection using samples collected from peach orchards, revealed that this rapid and simple technique is an effective way to identify the four viruses simultaneously. The mRT-PCR assay described in this study was developed for the simultaneous detection of four peach viruses from infected peach samples is reliable and sensitive. In contrast to conventional uniplex RT-PCR, mRT-PCR is more efficient, reducing costs, time and handling when testing large numbers of samples. This rapid and simple method is useful for large-scale surveys of viruses that infect peach. © 2013 The Society for Applied Microbiology.
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Evaluación de anemia ferropénica en la primera infancia del Municipio de Sonsón-Antioquia, 2007
Directory of Open Access Journals (Sweden)
Tulia Isabel Castillo Salgado
2014-12-01
Full Text Available Objetivo: Determinar la prevalencia de anemia por deficiencia de hierro en niños de la primera infancia del municipio de Sonsón – Antioquia, en el año 2007. Metodología: Se realizó un estudio descriptivo transversal en 391 niños de 0 a 84 meses de edad del Municipio de Sonsón, Antioquia. Se estudiaron los parámetros de hemoglobina y ferritina sérica utilizando puntos de corte de la Organización Mundial de la Salud (OMS. Se calcularon las medidas de frecuencia y de resumen y los intervalos de confianza para proporciones y las pruebas de estadística paramétrica y no paramétrica. Resultados: La prevalencia de anemia ferropénica fue de 4,3% y la media de ferritina sérica y de hemoglobina para la población del estudio fue de 29,9 ng/mL y 12,6 g/dL respectivamente. Adicionalmente se halló significancia estadística de la prevalencia de anemia ferropénica por grupo de edad (p<0,0001; pero no se encontró significancia estadística de la prevalencia de anemia con el sexo (p= 1,0000. Conclusión: La prevalencia de anemia ferropénica en los niños de la primera infancia del Municipio de Sonsón fue baja de acuerdo con los parámetros de hemoglobina y ferritina sérica establecidos por la OMS, hay variación de la anemia ferropénica por grupo de edad pero no por sexo.
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Peces del Noroeste del Ecuador
OpenAIRE
Barriga, Ramiro
1994-01-01
La ictiofauna del occidente del Ecuador es poco conocida. Los peces del noroccidente son diferentes a los del suroccidente del Ecuador. 34 familias y 82 especies fueron colectadas que equivale al 11 % de las especies de peces continentales registradas en el Ecuador. Icteogeográficamente se sabe que la costa ecuatoriana posee dos provincias: la del Pacifico Norte y la del Guayas, se determinó que el límite de las dos provincias es el río Santiago ya que las especies del mencionado río so...
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Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay.
Science.gov (United States)
Lyu, Xiaodong; Wang, Xianwei; Zhang, Lina; Chen, Zhenzhu; Zhao, Yu; Hu, Jieying; Fan, Ruihua; Song, Yongping
2017-07-25
Fusion genes generated from chromosomal translocation play an important role in hematological malignancies. Detection of fusion genes currently employ use of either conventional RT-PCR methods or fluorescent in situ hybridization (FISH), where both methods involve tedious methodologies and require prior characterization of chromosomal translocation events as determined by cytogenetic analysis. In this study, we describe a real-time quantitative reverse transcription PCR (qRT-PCR)-based multi-fusion gene screening method with the capacity to detect 22 fusion genes commonly found in leukemia. This method does not require pre-characterization of gene translocation events, thereby facilitating immediate diagnosis and therapeutic management. We performed fluorescent qRT-PCR (F-qRT-PCR) using a commercially-available multi-fusion gene detection kit on a patient cohort of 345 individuals comprising 108 cases diagnosed with acute myeloid leukemia (AML) for initial evaluation; remaining patients within the cohort were assayed for confirmatory diagnosis. Results obtained by F-qRT-PCR were compared alongside patient analysis by cytogenetic characterization. Gene translocations detected by F-qRT-PCR in AML cases were diagnosed in 69.4% of the patient cohort, which was comparatively similar to 68.5% as diagnosed by cytogenetic analysis, thereby demonstrating 99.1% concordance. Overall gene fusion was detected in 53.7% of the overall patient population by F-qRT-PCR, 52.9% by cytogenetic prediction in leukemia, and 9.1% in non-leukemia patients by both methods. The overall concordance rate was calculated to be 99.0%. Fusion genes were detected by F-qRT-PCR in 97.3% of patients with CML, followed by 69.4% with AML, 33.3% with acute lymphoblastic leukemia (ALL), 9.1% with myelodysplastic syndromes (MDS), and 0% with chronic lymphocytic leukemia (CLL). We describe the use of a F-qRT-PCR-based multi-fusion gene screening method as an efficient one-step diagnostic procedure as an
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Variación regional del crecimiento y estado nutricional: Jujuy
Directory of Open Access Journals (Sweden)
Bejarano, Ignacio
2005-01-01
Full Text Available La descripción y el análisis del crecimiento humano han ocupado gran parte de las investigaciones de antropobiólogos, médicos y biólogos humanos, entre otros. En Argentina si bien se dispone de información de prevalencias de malnutrición, existe dificultad en la comparación debido a que en la mayor parte de los casos hay disparidad en las técnicas y/o metodologías empleadas. El objetivo del presente trabajo es analizar el estado nutricional de la población de Jujuy a fin de disponer de elementos diagnósticos que permitan su contrastación con otras provincias argentinas. Peso corporal (kg. y talla (cm. fueron medidos en una muestra de 27848 niños y adolescentes (13550 varones y 14298 mujeres durante 2003. Con los datos obtenidos se analizaron los indicadores Peso/Edad (P/E, Peso/Talla (P/T, Talla/Edad (T/E e Indice de Masa Corporal (IMC. La prevalencia de cada indicador se calculó siguiendo los puntos de corte recomendados por la OMS: para bajo P/E, T/E y P/T se utilizó el percentilo 5, para sobrepeso (S el intervalo percentilar 85-95 (>P85 IMC P95. Los resultados obtenidos indicaron las siguientes prevalencias totales: Desnutrición: 26,1%, Bajo P/E: 15,6%, Baja T/E: 23,4% Bajo P/T: 7,0%, S: 8,5% y O: 4,3%. Los resultados obtenidos indican que, de acuerdo a la OMS, la población evaluada pertenece a un grupo de prevalencia mediana tanto para Bajo P/E como para Baja T/E, siendo moderados los porcentajes de sobrepeso y obesidad.
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Correlación y concordancia entre el peso fetal estimado por ecografía y el peso real obtenido por báscula de los recién nacidos a término en el Hospital Vicente Corral Moscoso durante el 2012
OpenAIRE
Becerra Pino, Iván Oliver
2013-01-01
Antecedentes: El ultrasonido prenatal permite el cálculo directo del tamaño del fetal y la predicción de su peso con un intervalo de confianza del 95%. Es importante conocer con anticipación el peso fetal, porque permite incrementar las probabilidades de sobrevivir que tiene un RN.- Objetivos: determinar la correlación y la concordancia entre el peso fetal estimado por ecografía y el peso real obtenido al nacer.- Materiales y métodos: Se realizó un diseño descriptivo observacional y trans...
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NASA SPoRT Modeling and Data Assimilation Research and Transition Activities Using WRF, LIS and GSI
Science.gov (United States)
Case, Jonathan L.; Blankenship, Clay B.; Zavodsky, Bradley T.; Srikishen, Jayanthi; Berndt, Emily B.
2014-01-01
weather research and forecasting ===== The NASA Short-term Prediction Research and Transition (SPoRT) program has numerous modeling and data assimilation (DA) activities in which the WRF model is a key component. SPoRT generates realtime, research satellite products from the MODIS and VIIRS instruments, making the data available to NOAA/NWS partners running the WRF/EMS, including: (1) 2-km northwestern-hemispheric SST composite, (2) daily, MODIS green vegetation fraction (GVF) over CONUS, and (3) NASA Land Information System (LIS) runs of the Noah LSM over the southeastern CONUS. Each of these datasets have been utilized by specific SPoRT partners in local EMS model runs, with select offices evaluating the impacts using a set of automated scripts developed by SPoRT that manage data acquisition and run the NCAR Model Evaluation Tools verification package. SPoRT is engaged in DA research with the Gridpoint Statistical Interpolation (GSI) and Ensemble Kalman Filter in LIS for soil moisture DA. Ongoing DA projects using GSI include comparing the impacts of assimilating Atmospheric Infrared Sounder (AIRS) radiances versus retrieved profiles, and an analysis of extra-tropical cyclones with intense non-convective winds. As part of its Early Adopter activities for the NASA Soil Moisture Active Passive (SMAP) mission, SPoRT is conducting bias correction and soil moisture DA within LIS to improve simulations using the NASA Unified-WRF (NU-WRF) for both the European Space Agency's Soil Moisture Ocean Salinity and upcoming SMAP mission data. SPoRT has also incorporated real-time global GVF data into LIS and WRF from the VIIRS product being developed by NOAA/NESDIS. This poster will highlight the research and transition activities SPoRT conducts using WRF, NU-WRF, EMS, LIS, and GSI.
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Kuu plaat : Märt Sults "Kõigest koolipapa...". Plaadid kauplusest Lasering
Index Scriptorium Estoniae
2005-01-01
Heliplaatidest : Märt Sults "Kõigest koolipapa...", Madonna "Confessions on a Dance Floor", Tõnis Mägi "Vestlus Hermanniga", Rammstein "Rosenrot", a-ha "Analogue", Darkness "One Way Ticket to Hell...And Back"
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Estimación de pequeñas perturbaciones en satélites geocéntricos como un problema inverso
Science.gov (United States)
Zadunaisky, P. E.
El movimiento geocéntrico de un satélite artificial es simulado automáticamente por un sistema de ecuaciones diferenciales de segundo orden que incluyen dos funciones perturbadoras. La primera representa el segundo término del potencial gravitatorio de la Tierra y la segunda corresponde al frenado atmosférico. Asumiendo como conocidas, por hipótesis o por mediciones, la posición y velocidad del satélite en instantes sucesivos se estiman las perturbaciones a intervalos sucesivos por un método determinístico. Para ilustrar el método y comprobar la precisión de nuestros resultados hemos simulado dos ejemplos incluyendo valores conocidos de las perturbaciones que luego comparamos con los resultados de nuestro método. El método puede aplicarse por ejemplo para el intervalo de una revolución satelital lo cual permitiría el diseño inmediato de una maniobra correctiva de la órbita satelital.
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Torm Balti merel / Märt Treier ; interv. Inge Pitsner
Index Scriptorium Estoniae
Treier, Märt, 1975-
2004-01-01
TV3 näitab saksa poliitilist põnevusfilmi "Balti torm" ("Baltic Storm"), mis kujutab parvlaev "Estonia" hukku : stsenarist, produtsent Jutta Rabe : režissöör Reuben Leder. Filmi eel on teles Märt Treieri saade "Tormist sündinud tormid"
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A dose-volume comparison of prostate cancer (PC) radiotherapy (RT) techniques for penile-structures (PNS) - a neglected critical organ in PC RT
International Nuclear Information System (INIS)
Dabrowski, Jolanta; Myrianthopoulos, Leon; Nguyen, Ai; Chen, George; Vijayakumar, Srinivasan
1996-01-01
Purpose/Objective: Three-dimensional conformal RT(3DCRT) is revolutionizing the use of RT in PC. Rectum and bladder, and in some studies femoral heads are included as critical structures (CS) in comparing rival plans in 3DCRT. Although RT-induced impotence is a major complication of conventional RT, with 30-50% incidence, to date no study has included PNS as a CS. This study is an attempt to remedy this deficiency in the 3DCRT planning in PC. Materials and Methods: After immobilization with Aquaplast, computed-tomography (CT) scans were obtained in supine treatment position from top of lumbar-3 vertebra to lesser-trochanter of the femora with 5-8mm slice-thicknesses; IV contrast was used in all patients. Prostate, seminal vesicles (GTV), and CSs were outlined, including PNS. Corpora cavernosa and bulbous spongiosum together were identified as PNS. Appropriate margins for CTV and PTV were used; total margin to the block from GTV was 2cm. Tumor-minimum doses were prescribed to the 100% isodose line. Dose-volume histograms (DVHs) were obtained to compare three different techniques: 1. Conventional 4 field box technique (4FC) with equal weighting; 2. Six field (2 laterals and 4 obliques [45 degrees from midline] (6FO), with 50% dose delivery from the laterals; and 3. Four equally weighted, non-axial fields [2 laterals and 2 inferior anterior obliques at 45 degree couch and gantry rotations] (4FN). Results: A total of 12 patients are included in the study. The mean and range of percentage volume of PNS receiving more than 30, 60, and 90% of the prescribed dose are shown in the table below: Box plots, such as the example shown above, were used to compare techniques overall. The 6-field coplanar technique treated the least PNS volume beyond a given dose, followed by 4FC and 4FN techniques. The order of least to maximum percent of PNS treated in most individual patients also followed the same trend. In the majority, 6FO and 4FN delivered relatively comparable doses to
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Helical Tomotherapy-Based STAT RT: Dosimetric Evaluation for Clinical Implementation of a Rapid Radiation Palliation Program
International Nuclear Information System (INIS)
McIntosh, Alyson; Dunlap, Neal; Sheng, Ke; Geezey, Constance; Turner, Benton; Blackhall, Leslie; Weiss, Geoffrey; Lappinen, Eric; Larner, James M.; Read, Paul W.
2010-01-01
Helical tomotherapy-based STAT radiation therapy (RT) uses an efficient software algorithm for rapid intensity-modulated treatment planning, enabling conformal radiation treatment plans to be generated on megavoltage computed tomography (MVCT) scans for CT simulation, treatment planning, and treatment delivery in one session. We compared helical tomotherapy-based STAT RT dosimetry with standard linac-based 3D conformal plans and standard helical tomotherapy-based intensity-modulated radiation therapy (IMRT) dosimetry for palliative treatments of whole brain, a central obstructive lung mass, multilevel spine disease, and a hip metastasis. Specifically, we compared the conformality, homogeneity, and dose with regional organs at risk (OARs) for each plan as an initial step in the clinical implementation of a STAT RT rapid radiation palliation program. Hypothetical planning target volumes (PTVs) were contoured on an anthropomorphic phantom in the lung, spine, brain, and hip. Treatment plans were created using three planning techniques: 3D conformal on Pinnacle 3 , helical tomotherapy, and helical tomotherapy-based STAT RT. Plan homogeneity, conformality, and dose to OARs were analyzed and compared. STAT RT and tomotherapy improved conformality indices for spine and lung plans (CI spine = 1.21, 1.17; CI lung = 1.20, 1.07, respectively) in comparison with standard palliative anteroposterior/posteroanterior (AP/PA) treatment plans (CI spine = 7.01, CI lung = 7.30), with better sparing of heart, esophagus, and spinal cord. For palliative whole-brain radiotherapy, STAT RT and tomotherapy reduced maximum and mean doses to the orbits and lens (maximum/mean lens dose: STAT RT = 2.94/2.65 Gy, tomotherapy = 3.13/2.80 Gy, Lateral opposed fields = 7.02/3.65 Gy), with an increased dose to the scalp (mean scalp dose: STAT RT = 16.19 Gy, tomotherapy = 15.61 Gy, lateral opposed fields = 14.01 Gy). For bony metastatic hip lesions, conformality with both tomotherapy techniques (CI = 1
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School Psychologists' Willingness to Implement RtI: The Role of Philosophical and Practical Readiness
Science.gov (United States)
Fan, Chung-Hau; Denner, Peter R.; Bocanegra, Joel O.; Ding, Yi
2016-01-01
After the change in IDEIA, different models of response to intervention (RtI) have been practiced widely in American school systems. School psychologists are in an important position to facilitate RtI practice and provide professional development in order to help their school systems successfully undergo this transformation. However, there is a…
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Pärt, Arvo: "Summa". Collage sur BACH. Fratres / Patric Wiklacz
Index Scriptorium Estoniae
Wiklacz, Patric
1997-01-01
Uuest heliplaadist "Pärt, Arvo: "Summa". Collage sur BACH. Fratres. Cantus in Memoriam Benjamin Britten. Summa. Festina Lente. Tabula rasa. Tapiola Sinfonietta / Jean-Jacques Kantorow". BIS-CD-834. 62:30 DDD
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Las rachas secas en el sector central de la cuenca del Duero
Directory of Open Access Journals (Sweden)
Miguel Ángel Luengo Ugidos
2002-01-01
Full Text Available El objetivo de este trabajo es la caracterización climática del sector más árido de la cuenca del Duero, prestando una especial atención a los intervalos sin precipitación (rachas secas, y la estimación de la probabilidad y frecuencia de estos períodos mediante técnicas estadísticas de aplicación sencilla. La precipitación media es inferior a los 400 mm y el número anual de días secos es elevado. Considerando día seco aquél con una precipitación ≤ 01 mm, la media es de 291, y la longitud media de las rachas secas 96 días. Elevando el umbral a 10 mm, el número de días secos al año asciende a 356 y la longitud media de las rachas a 401 días. Estos datos, junto al análisis de la duración, intensidad y probabilidad de las rachas secas máximas anuales, confirman que la zona estudiada está más próxima a la realidad de los observatorios mediterráneos que a la de los atlánticos, a pesar de su ubicación en la mitad septentrional de la Península Ibérica. Finalmente, tanto las cadenas de Markov de primer orden, como la distribución de frecuencias de Gumbel, se han mostrado como técnicas adecuadas para la estimación de la probabilidad y frecuencia de las rachas secas, respectivamente
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El clima de la vertiente del Pacífico de los Andes Centrales y sus implicaciones geomorfológicas El clima de la vertiente del Pacífico de los Andes Centrales y sus implicaciones geomorfológicas
Directory of Open Access Journals (Sweden)
Jose Úbeda Palenque
2008-12-01
ífico de los Andes Centrales, entre 15°S y 19°S, debido a que los procesos mediante los cuales se desarrolla el modelado del relieve o morfogénesis están controlados por las variables termo-pluviométricas y la fito-estabilización. Además, la geodinámica interna de la región se encuentra igualmente relacionada con las condiciones climáticas, que determinan la cuantía del aporte de sedimentos a la fosa oceánica (Kulm et al. 1977, afectando a la tasa de erosión por subducción en el plano de contacto entre las placas y a la composición de los magazas que se forman bajo el erógeno andino. En esta ponencia se analizan los efectos del clima en la geodinámica externa, en un marco teórico en el que se considera que un sistema morfoclimático es un conjunto integrado por los agentes y procesos morfogenéticos que realizan el modelado del relieve en un territorio, en función de sus características bioclimáticas, y un dominio o piso morfoclimático es el ámbito espacial en el que se desarrolla un determinado sistema morfoclimático.En primer lugar se realiza una presentación del clima en los Andes Centrales, los factores que controlan su configuración media y sus principales características, presentando diagramas elaborados a partir de datos termo-pluviométricos recogidos en una serie de observatorios regularmente distribuidos por la región. En segundo lugar, se diferencias dos dominios climáticos, uno en el altiplano, relacionado con las precipitaciones de componente amazónica, y otro en la rampa que desciende hacia el océano y en la costa del Pacífico, asociado a la situación anticiclónica permanente al O de la cordillera. En tercer lugar, se propone una compartimentación de la fachada occidental de la cordillera en intervalos altitudinales o pisos bioclimáticos, para cuya definición se han empleado como bioindicadores la ausencia de vegetación (por causas térmicas o hídricas o su presencia con un cambio altitudinal de facies (supraforestal
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Combinación de Valores de Longitud del Día (LOD) según ventanas de frecuencia
Science.gov (United States)
Fernández, L. I.; Arias, E. F.; Gambis, D.
El concepto de solución combinada se sustenta en el hecho de que las diferentes series temporales de datos derivadas a partir de distintas técnicas de la Geodesia Espacial son muy disimiles entre si. Las principales diferencias, fácilmente detectables, entre las distintas series son: diferente intervalo de muestreo, extensión temporal y calidad. Los datos cubren un período reciente de 27 meses (julio 96-oct. 98). Se utilizaron estimaciones de la longitud del día (LOD) originadas en 10 centros operativos del IERS (International Earth Rotation Service) a partir de las técnicas GPS (Global Positioning System) y SLR (Satellite Laser Ranging). La serie temporal combinada así obtenida se comparó con la solución EOP (Parámetros de la Orientación Terrestre) combinada multi-técnica derivada por el IERS (C04). El comportamiento del ruido en LOD para todas las técnicas mostró ser dependiente de la frecuencia (Vondrak, 1998). Por esto, las series dato se dividieron en ventanas de frecuencia, luego de haberles removido bies y tendencias. Luego, se asignaron diferentes factores de peso a cada ventana discriminando por técnicas. Finalmente estas soluciones parcialmente combinadas se mezclaron para obtener la solución combinada final. Sabemos que la mejor solución combinada tendrá una precisión menor que la precisión de las series temporales de datos que la originaron. Aun así, la importancia de una serie combinada confiable de EOP, esto es, de una precisión aceptable y libre de sistematismos evidentes, radica en la necesidad de una base de datos EOP de referencia para el estudio de fenómenos geofísicos que motivan variaciones en la rotación terrestre.
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REEMPLAZO DEL CUARZO POR CENIZA DE TAMO DE ARROZ EN LA MANUFACTURA DE GRES PORCELÁNICO
Directory of Open Access Journals (Sweden)
Jerrison Delgado Trujillo
Full Text Available En este documento se presentan los resultados de una investigación basada en el uso de un material alternativo como lo es la ceniza de tamo de arroz (CTA, en sustitución del material de relleno (cuarzo utilizado para la manufactura de gres porcelánico. Para ello, fue preparada una mezcla estándar (RS_0 (15 % cuarzo, 45 % feldespato, 25 % arcilla caolinítica y 15 % caolín, y dos mezclas más donde la CTA sustituyó al cuarzo en porcentajes del 25 % y 50 % (RS_25 y RS_50, respectivamente. Especímenes de las pastas obtenidas fueron moldeados, secados, y cocidos. A partir de los valores de absorción de agua y resistencia a la flexión, los especímenes cocidos de las mezclas estándar (RS_0 y aquellas con adición de CTA RS_25 y RS_50, fueron clasificados como baldosas de gres porcelánico prensadas en seco pertenecientes al grupo BIa, conforme a la normativa ISO 13006 (resistencia a flexión > 35 MPa y absorción de agua ≤ 0,5 %; sin embargo, en aquellos especímenes cocidos RS_50 el intervalo óptimo de cocción más estrecho y el fenómeno de hinchamiento deben ser tenidos en cuenta a la hora de establecer la temperatura óptima de cocción; lo que indica que debe tenerse un mayor control del proceso de cocción de estas mezclas. Los resultados permitieron concluir que la CTA bajo las condiciones de obtención, sí reemplaza parcialmente el material de relleno utilizado en la elaboración de gres porcelánico.
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Propuesta de un plan de desarrollo integral del guanábano (Annona muricata L. en el estado de Veracruz México
Directory of Open Access Journals (Sweden)
Librado Vidal Hernández
2014-01-01
Full Text Available El estado de Veracruz tiene una superficie de 71' 227 km². Cuenta con una zona potencialmente apta para el cultivo del guanábano de 18' 440 ha, (0.21%, una zona medianamente propicia de 3' 645 324 ha (51.30% y una zona no apropiada de 3' 458 862 ha, (48.44%. Existen 20 municipios productores de guanábano en el estado de Veracruz. Actualmente la demanda por este producto ha permitido su incremento en superficie estimándose en 800 ha, en estos últimos años. Con un rendimiento aproximado de 5.0 ton/ha, por debajo de la media nacional que es de 6.5 ton/ha, esto refleja la poco tecnología empleada en el manejo del cultivo. Lamentablemente el desarrollo de este frutal en Veracruz se ha realizado de una manera desordenada. Todo ello, sin ninguna planeación y sin un estudio sobre un ordenamiento agroecológico a fin de detectar áreas potencialmente aptas para este cultivo. A pesar de toda esta complejidad se ha llegado a considerar como un frutal digno de atención por las posibilidades agroindustriales que representa. En general son tres los principales puntos prioritarios a tomar en cuenta para esta estrategia de desarrollo: Primero las características genéticas del material de propagación. Segundo las condiciones de sanidad de las plantas, principal factor que podría ser limitativo para el desarrollo del guanábano. Tercero la tecnología de producción. Existe desconocimiento en la lámina e intervalo riego, época; dosis y fuente de fertilización; época y tipo de poda. Existe una gran fortaleza en su aprovechamiento integral de este frutal: comercial, industrial, medicinal, farmacéutico, fitotóxico, alimenticio, entre otras propiedades.
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DINÁMICA SEROLÓGICA DEL VIRUS DE BRONQUITIS INFECCIOSA EN UNA GRANJA DE POLLO DE ENGORDE DEL DEPARTAMENTO DE CUNDINAMARCA
Directory of Open Access Journals (Sweden)
DC Álvarez
2009-01-01
Full Text Available El virus de bronquitis infecciosa (IBV causa una enfermedad altamente contagiosa, distribuida mundialmente, que conlleva graves pérdidas económicas. En algunas oportunidades se asocia con otras entidades como los virus de las enfermedades de Gumboro y de Newcastle, Mycoplasma gallisepticum y Escherichia coli. La alta variabilidad genética del virus ha generado una gran cantidad de cepas virales con diferentes cuadros clínicos. El objetivo del trabajo fue evaluar la dinámica de anticuerpos del IBV en aves vacunadas y no vacunadas contra IBV, alojadas en una explotación de pollo de engorde donde se detectó el agente por RT-PCR, en Fusagasugá, Colombia, y aves vacunadas en semiaislamiento en Bogotá. Para esto se organizaron 3 grupos de aves (Ross 308 de 1 día de edad (44 aves/grupo, las cuales fueron vacunadas con un virus vivo atenuado, cepa Massachusetts H120, y se evaluó la respuesta inmune a través de la prueba de Elisa. Desde el primer día hasta el día 24 de edad se observó una disminución progresiva de los títulos de anticuerpos en los tres grupos, aunque en las aves vacunadas y no vacunadas mantenidas en granja se observaron niveles de anticuerpos superiores al grupo en condiciones de semiaislamiento. A partir del día 28 en las aves alojadas en campo se incrementaron levemente los títulos hasta final de ciclo. El leve aumento en el nivel de anticuerpos puede ser consecuencia de exposición al virus vacunal que generó reversión de patogenicidad, persistencia viral o una exposición tardía al virus de campo.
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Uss uuristab inimese südame kallal / Kärt Hellerma
Index Scriptorium Estoniae
Hellerma, Kärt, 1956-
2003-01-01
Arvustus: [Õnnepalu, Tõnu] Tode, Emil. Raadio. Tallinn : Eesti Keele Sihtasutus, 2002. Ilmunud ka kogumikus: Hellerma, Kärt. Kohanenud kirjandus : valik kirjanduskriitikat 1987-2006. Eesti Keele Sihtasutus : Tallinn, 2006. Lk. 47-53
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Intervalos de observações com diferentes escalas de tempo no comportamento ingestivo de vacas leiteiras confinadas Intervals between observations at different time scales in the feeding behavior of dairy confined cows
Directory of Open Access Journals (Sweden)
Fabrício Bacelar Lima Mendes
2011-09-01
Full Text Available Objetivou-se analisar as diferentes escalas de tempo para determinar qual o intervalo mais adequado no estudo do comportamento ingestivo de vacas leiteiras confinadas. O experimento foi conduzido na fazenda Água Azul, Município de Macarani-Bahia, no período de outubro a novembro de 2005. Foram utilizadas 12 vacas mestiças Holandês, apresentando, em média, 478,5 15,89kg de peso corporal. Os tratamentos do presente estudo foram os intervalos (cinco, 10; 15; 20 e 30 minutos, observados durante o comportamento ingestivo. A observação do comportamento ocorreu no penúltimo dia de cada período de 12 dias durante 24 horas. Procedeu-se a análise de variância e a aplicação do teste de Dunnett, adotando-se 0,05 como nível crítico de probabilidade. Não houve efeito significativo das escalas de registro do tempo de alimentação, ruminação, ócio e para as eficiências de alimentação e ruminação. Para as variáveis dos números de períodos de alimentação, ruminação e ócio e os tempos por período de alimentação, ruminação e ócio, as escalas de 10, 15, 20 e 30 minutos foram diferentes quando comparadas com o intervalo de observação de cinco minutos. As coletas totais dos tempos de alimentação ruminação e ócio, e as eficiências de alimentação e ruminação nos diferentes nutrientes podem ser realizadas com intervalo de observação de até 30 minutos. Para a discretização das séries temporais do comportamento ingestivo recomenda-se a escala de cinco minutos entre as observações.This study aimed to analyze different time scales to determine the most appropriate interval in the study of ingestive behavior of confined dairy cows. The experiment was conducted at the farm Blue Water, city of Macarani-Bahia, in the period from October to November 2005. 12 crossbred Holstein cows were used, with an average body weight of 478.5 15.89kg. The treatments of this study were the intervals (five; 10; 15; 20 and 30
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OR.NET RT: how service-oriented medical device architecture meets real-time communication.
Science.gov (United States)
Pfeiffer, Jonas H; Kasparick, Martin; Strathen, Benjamin; Dietz, Christian; Dingler, Max E; Lueth, Tim C; Timmermann, Dirk; Radermacher, Klaus; Golatowski, Frank
2018-02-23
Today's landscape of medical devices is dominated by stand-alone systems and proprietary interfaces lacking cross-vendor interoperability. This complicates or even impedes the innovation of novel, intelligent assistance systems relying on the collaboration of medical devices. Emerging approaches use the service-oriented architecture (SOA) paradigm based on Internet protocol (IP) to enable communication between medical devices. While this works well for scenarios with no or only soft timing constraints, the underlying best-effort communication scheme is insufficient for time critical data. Real-time (RT) networks are able to reliably guarantee fixed latency boundaries, for example, by using time division multiple access (TDMA) communication patterns. However, deterministic RT networks come with their own limitations such as tedious, inflexible configuration and a more restricted bandwidth allocation. In this contribution we overcome the drawbacks of both approaches by describing and implementing mechanisms that allow the two networks to interact. We introduce the first implementation of a medical device network that offers hard RT guarantees for control and sensor data and integrates into SOA networks. Based on two application examples we show how the flexibility of SOA networks and the reliability of RT networks can be combined to achieve an open network infrastructure for medical devices in the operating room (OR).
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TMPA Products 3B42RT & 3B42V6: Evaluation and Application in Qinghai-Tibet Plateau
Science.gov (United States)
Hao, Z.; Sun, L.; Wang, J.
2012-04-01
Hydrological researchers in Qinghai-Tibet Plateau tend to be haunted by deficiency of station gauged precipitation data for the sparse and uneven distribution of local meteorological stations. Fortunately, alternative data can be obtained from TRMM (Tropic Rainfall Measurement Mission) satellite. Preliminary evaluation and necessary correction of TRMM satellite rainfall products is required for the sake of reliability and suitability considering that TRMM precipitation is unconventional and natural condition in Qinghai-Tibet Plateau is unusually complicated. 3B42RT and 3B42V6 products from TRMM Multisatellite Precipitation Analysis(TMPA) are evaluated in northeast Qinghai-Tibet Plateau with 50 stations quality-controlled gauged daily precipitation as the benchmark precipitation set. It is found that the RT data overestimates the actual precipitation greatly while V6 only overestimates it slightly. RT data shows different seasonal and inter-annual accuracies. Summer and autumn see better accuracies than winter and spring and wet years see higher accuracies than dry years. Latitude is believed to be an important factor that influences the accuracy of satellite precipitation. Both RT and V6 can reflect the general pattern of the spatial distribution of precipitation even though RT overestimates the quantity greatly. A new parameter, accumulated precipitation weight point (APWP), was introduced to describe the temporal-spatial pattern evolution of precipitation. The APWP of both RT and V6 were moving from south to north in the past decade, but they are all in the west of station gauged precipitation APWP(s).V6 APWP track fit gauged precipitation perfectly while RT APWP track has over-exaggerated legs, indicating that spatial distribution of RT precipitation experienced unreasonable sharp changes. A practical and operational procedure to correct satellite precipitation data is developed. For RT, there are two steps. Step 1, the downscaling, original daily precipitation
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Intervals between observations for ingestive behavior evaluation of young bulls in feedlot/ Intervalo de tempo entre observações para avaliação do comportamento ingestivo de tourinhos em confinamento
Directory of Open Access Journals (Sweden)
Willian Gonçalves do Nascimento
2008-08-01
Full Text Available The objective of this experiment was to study the intervals of time for observing the ingestive behavior of 36 young crossbred bulls in feedlot. The animals were housed in individual stalls, in a completely randomized design, with three treatments (sorghum silage with 1.0 % concentrate, sugar cane with 1.0 % concentrate and sugar cane with 1.2 % concentrate. Five different intervals between observations were studied. The evaluated parameters were: feeding, rumination and idle, time and frequency, dry matter and neutral detergent fiber intake, total chewing time, feeding and rumination efficiency of dry matter and neutral detergent fiber, as well as, the percentage of the time in that the animals were laying during rumination or idle. The intervals of time did not differ (P > 0.05 for analyzed variable of the time and efficiency. The percentage of time in that the animals were laid while ruminating, was 91 %. The frequencies of feeding, rumination and idle, differed (P Objetivou-se com o experimento estudar intervalos de tempo para avaliação do comportamento ingestivo de tourinhos confinados. Foram utilizados 36 animais, alojados em baias individuais, em delineamento inteiramente casualizado, com três tratamentos (silagem de sorgo com 1,0 % de concentrado, cana-deaçúcar com 1,0 % de concentrado e com 1,2 % de concentrado. Foram comparadas cinco escalas de intervalos de observações. Avaliou-se o tempo e freqüência de alimentação, ruminação e ócio, sendo que destes dois últimos itens calculou-se a percentagem do tempo que os animais permaneciam deitados, ingestão de matéria seca e de fibra em detergente neutro, tempo de mastigação total e eficiência de alimentação e de ruminação da matéria seca e da fibra em detergente neutro. Não se observou diferença significativa (p > 0,05 entre as escalas de tempo analisadas para as variáveis relacionadas a tempo e eficiência. A percentagem de tempo que os animais permaneceram
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Unequal distribution of RT-PCR artifacts along the E1-E2 region of Hepatitis C virus.
Science.gov (United States)
Domingo-Calap, Pilar; Sentandreu, Vicente; Bracho, Maria Alma; González-Candelas, Fernando; Moya, Andrés; Sanjuán, Rafael
2009-10-01
Although viral variability studies have focused traditionally on consensus sequences, the relevance of molecular clone sequences for studying viral evolution at the intra-host level is being increasingly recognized. However, for this approach to be reliable, RT-PCR artifacts do not have to contribute excessively to the observed variability. Molecular clone sequences were obtained from an in vitro transcript to estimate the maximum error rate associated to RT-PCR for the Hepatitis C virus (HCV) E1-E2 region. On average, the frequency of RT-PCR errors was one order of magnitude lower than the level of intra-host genetic variability observed in samples from an HCV outbreak. However, RT-PCR errors were not distributed evenly along the E1-E2 region and were concentrated heavily in the hypervariable region 2 (HVR 2). Although it is concluded that RT-PCR molecular clone sequences are reliable, these results warn against extrapolation of RT-PCR error rates to different genome regions. The data suggest that the RNA sequence context or secondary structure can determine the fidelity of in vitro transcription or reverse transcription. Potentially, these factors might also modify the fidelity of the viral polymerase.
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The Reaumuria trigyna transcription factor RtWRKY1 confers tolerance to salt stress in transgenic Arabidopsis.
Science.gov (United States)
Du, Chao; Zhao, Pingping; Zhang, Huirong; Li, Ningning; Zheng, Linlin; Wang, Yingchun
2017-08-01
Reaumuria trigyna (R. trigyna) is an endangered small shrub endemic to the Eastern Alxa-Western Ordos area in Inner Mongolia, China. Based on R. trigyna transcriptome data, the Group I WRKY transcription factor gene RtWRKY1 was cloned from R. trigyna. The full-length RtWRKY1 gene was 2100bp, including a 1261-bp open reading frame (ORF) encoding 573 amino acids. RtWRKY1 was mainly expressed in the stem and was induced by salt, cold stress, and ABA treatment. Overexpression of RtWRKY1 in Arabidopsis significantly enhanced the chlorophyll content, root length, and fresh weight of the transgenic lines under salt stress. RtWRKY1 transgenic Arabidopsis exhibited higher proline content, GSH-PX, POD, SOD, and CAT activities, and lower MDA content, Na + content, and Na + /K + ratio than wild-type Arabidopsis under salt stress conditions. Salt stress affected the expression of ion transport, proline biosynthesis, and antioxidant related genes, including AtAPX1, AtCAT1, AtSOD1, AtP5CS1, AtP5CS2, AtPRODH1, AtPRODH2, and AtSOS1 in transgenic lines. RtWRKY1 confers tolerance to salt stress in transgenic Arabidopsis by regulating plant growth, osmotic balance, Na + /K + homeostasis, and the antioxidant system. Copyright © 2017 Elsevier GmbH. All rights reserved.
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Development of duplex RT-PCR-ELISA for the simultaneous detection of hepatitis A virus and hepatitis E virus.
Science.gov (United States)
Tahk, Hongmin; Lee, Min Hwa; Lee, Kang Bum; Cheon, Doo-Sung; Choi, Changsun
2011-07-01
This study aimed to develop a specific and sensitive duplex reverse transcription polymerase chain reaction enzyme-linked immunosorbent assay (duplex RT-PCR-ELISA) for hepatitis A virus (HAV) and hepatitis E virus (HEV). Duplex RT-PCR-ELISA could detect and differentiate HAV and HEV with specific probes. When ELISA technique was used to detect probe-bound RT-PCR products, duplex RT-PCR-ELISA could detect as little as 0.1 ng/μL HAV and HEV from clinical samples. Human norovirus, enterovirus, poliovirus, murine norovirus and feline calicivirus were used for the specificity test; all were negative. Therefore duplex RT-PCR-ELISA can be used for the simultaneous detection of HAV and HEV in contaminated fecal samples. Copyright © 2011 Elsevier B.V. All rights reserved.
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Comportamiento del asma bronquial en la edad pediátrica
Directory of Open Access Journals (Sweden)
Lilian Jiménez Fontao
2001-02-01
Full Text Available Se realizó un estudio observacional, analítico, con un modelo de diseño del tipo casos y controles en la población de niños que acuden al Círculo Infantil "Sueños del Ché", con el fin de determinar la posible influencia de algunos factores de riesgo para padecer de asma bronquial. La investigación se realizó durante el curso escolar 1996-1997. El material de estudio quedó conformado por 166 niños, 21 de los cuales eran asmáticos (GE, el resto (145 eran los supuestamente sanos y conformaron el grupo control (GC. Como instrumento epidemiológico se utilizó el riesgo relativo (RR y su intervalo de confianza al 95 %, así como la prueba de Chi cuadrado. El sexo masculino predominó entre los enfermos y las manifestaciones alérgicas fueron más frecuentes entre los niños enfermos, así como en aquellos cuyos familiares era fumadores o que padecían de asma bronquial. El ketotifeno consiguió el 50 % de mejoría entre los enfermos, y la presencia de vectores y/o animales en el hogar no resultó un factor importante a considerarAn observational analytic case-control study was conducted in the population of children attending the "Sueños del Ché" Day Care Center in order to determine the possible influence of some risk factors on bronchial asthma. The investigation was made during the academic course 1996-1997. 166 children, 21 of whom were asthmatics (SG, and other 145 apparently sound (CG took part in the study. The relative risk and its confidence interval of 95 %, as well as the Chi square test were used as an epidemiological tool. Males prevailed among the sick and the allergic manifestations were more common in ill children and in those whose relatives were smokers or suffered from bronchial asthma. An improvement of 50 % was attained with the admnistration of ketotifen. The presence of vectors and/or animals at home was not an important factor to be considered
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Biomarker discovery for colon cancer using a 761 gene RT-PCR assay
Directory of Open Access Journals (Sweden)
Hackett James R
2007-08-01
Full Text Available Abstract Background Reverse transcription PCR (RT-PCR is widely recognized to be the gold standard method for quantifying gene expression. Studies using RT-PCR technology as a discovery tool have historically been limited to relatively small gene sets compared to other gene expression platforms such as microarrays. We have recently shown that TaqMan® RT-PCR can be scaled up to profile expression for 192 genes in fixed paraffin-embedded (FPE clinical study tumor specimens. This technology has also been used to develop and commercialize a widely used clinical test for breast cancer prognosis and prediction, the Onco typeDX™ assay. A similar need exists in colon cancer for a test that provides information on the likelihood of disease recurrence in colon cancer (prognosis and the likelihood of tumor response to standard chemotherapy regimens (prediction. We have now scaled our RT-PCR assay to efficiently screen 761 biomarkers across hundreds of patient samples and applied this process to biomarker discovery in colon cancer. This screening strategy remains attractive due to the inherent advantages of maintaining platform consistency from discovery through clinical application. Results RNA was extracted from formalin fixed paraffin embedded (FPE tissue, as old as 28 years, from 354 patients enrolled in NSABP C-01 and C-02 colon cancer studies. Multiplexed reverse transcription reactions were performed using a gene specific primer pool containing 761 unique primers. PCR was performed as independent TaqMan® reactions for each candidate gene. Hierarchal clustering demonstrates that genes expected to co-express form obvious, distinct and in certain cases very tightly correlated clusters, validating the reliability of this technical approach to biomarker discovery. Conclusion We have developed a high throughput, quantitatively precise multi-analyte gene expression platform for biomarker discovery that approaches low density DNA arrays in numbers of
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Obtención del coeficiente de carbono orgánico para diesel a través de isotermas de adsorción
Directory of Open Access Journals (Sweden)
SANTIAGO ALONSO CARDONA GALLO
2007-01-01
Full Text Available En este trabajo se presentan los valores experimentales del coeficiente de sorción carbono orgánico, Koc, para diesel combustible de producción mexicana el cual es una mezcla de compuestos y cuyo valor no se reporta en la literatura especializada, solo se dan a conocer datos para compuestos individuales. El valor de Koc es constante para un mismo compuesto en un rango amplio de sedimentos y suelos. El coeficiente de distribución, Kd de la mayoría de los compuestos orgánicos se incrementa con la fracción de carbono orgánico, foc, implicando que otra opción para estimar Kd es a través del coeficiente de sorción Koc y foc mediante la relación Kd=foc Koc. Los valores de Kd se determinaron por medio de isotermas adsorción, para lo cual se probaron cinco tipos de suelos diferentes, cinco concentraciones de diesel en reactores intermitentes y se modelo el proceso con la ecuación de Freundlich considerando n=1 (concentraciones bajas para los ajustes lineales. Se obtuvieron valores de Koc en un rango de 44.82 a 55.62 mL/g. Se realizó el análisis de varianza y se aplicó la prueba de intervalos múltiples de Duncan para determinar la diferencia entre las medias de los Koc obtenidos. Gráficamente se presentó la influencia de la foc en la adsorción del diesel
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Detección y cuantificación del Potato mop-top virus (PMTV) en Colombia mediante qRT-PCR
OpenAIRE
Nevar García Bastidas; Pablo Gutiérrez Sánchez; Mauricio Marín Montoya
2013-01-01
El Potato mop-top virus (PMTV) es uno de los virus re-emergentes en cultivos de papa en Colombia. Es transmitido por Spongospora subterranea, el agente causal de la sarna polvosa. La detección del PMTV presenta dificultades debido a su distribución irregular en las plantas, bajo título y movimiento sistémico como ARN desnudo. Con el fin de ampliar el rango de herramientas disponibles para detectar el PMTV en los programas de certificación de tubérculo-semilla, en este estudio se evaluó la pru...
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Pierādījumu vērtēšana kriminālprocesā
OpenAIRE
Griķe, Madara
2011-01-01
Pierādījumu vērtēšana ir viena no būtiskākajām valsts atzīto un aizsargāto cilvēka pamattiesību garantijām kriminālprocesā, tā nosaka tiesas nolēmuma tiesiskumu un pamatotību. Attiecībā uz pierādījumu vērtēšanu likumā nav konkrēta panta, ir reglamentēti pierādīšanas teorijas centrālie jēdzieni, principi un prasības, pēc kurām jāatbilst pierādījumiem un pēc kurām tie jāvērtē. Autore darbā pievēršas sastopamām problēmām praksē, kas saistītas ar pierādījumu vērtēšanu. Autore aktualizē jautājumu...
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Arvo Pärt ajas ja ajatuses / Igor Garšnek
Index Scriptorium Estoniae
Garšnek, Igor, 1958-
2010-01-01
Mõtteid Arvo Pärdi muusikast ajendatuna ETVs 2. septembril kavas olnud saadetest: "Arvo Pärt Istanbulis " ja "Arvo Pärdi autorikontsert Istanbulis", mis kajastasid 7. juunil Hagia Irene kirikus esiettekandel tulnud helilooja uusimat teost "Aadama itk"
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Factores de riesgo para la enteropatía parasitaria en niños del municipio Las Tunas
Directory of Open Access Journals (Sweden)
Arley Fajardo Ochoa
2015-11-01
Full Text Available Se realizó un estudio analítico de tipo caso control, con el objetivo de determinar los factores de riesgo para la enteropatía parasitaria, como principal causa del síndrome de malabsorción intestinal en niños del municipio de Las Tunas durante el año 2011. El mismo fue realizado en el Hospital Pediátrico Provincial “Mártires de Las Tunas”, con una muestra de 87 pacientes que formaron parte del grupo de estudio, cuyos datos se recolectaron de la consulta médica de gastroenterología en el policlínico de consulta externa de dicha unidad asistencial y 174 en el grupo control, tomados de pacientes que acudieron a la consulta de urología, paralela en tiempo y contigua a la de gastroenterología. A cada grupo se le realizó un interrogatorio y examen físico exhaustivo, que permitió recoger todos los posibles factores de riesgo existentes. Para la determinación de los factores de riesgo se utilizaron los paquetes de programas estadísticos Epinfo versión 6, en el que se realizaron análisis univariados y se evaluó el ODDS RATIO, intervalo de confianza y probabilidad. El procesamiento de los datos se realizó con un 95% de confiabilidad. Los factores de riesgo asociados a la enteropatía parasitaria fueron: pertenecer al sexo masculino, los hábitos higiénicos inadecuados, estado nutricional inadecuado, la ingestión de agua sin hervir y la evacuación de excretas no sanitarias. Se recomienda realizar estudios analíticos para determinar la asociación de otros factores de riesgo, con vistas a establecer programas de intervención en estos pacientes.
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Propidium monoazide reverse transcription PCR and RT-qPCR for detecting infectious enterovirus and norovirus
Science.gov (United States)
Presently there is no established cell line or small animal model that allows for the detection of infectious human norovirus. Current methods based on RT-PCR and RT-qPCR detect both infectious and non-infectious virus and thus the conclusions that may be drawn regarding the publ...
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Investigations for a multi-marker RT-PCR to improve sensitivity of disseminated tumor cell detection.
NARCIS (Netherlands)
Vlems, F.A.; Diepstra, J.H.S.; Cornelissen, I.M.; Ligtenberg, M.J.L.; Wobbes, Th.; Punt, C.J.A.; Krieken, J.H.J.M. van; Ruers, T.J.M.; Muijen, G.N.P. van
2003-01-01
BACKGROUND: In order to develop a multi-marker RT-PCR, which as such may be more sensitive than a single marker assay for the detection of disseminated tumor cells, we evaluated six RT-PCR markers: cytokeratin 20 (CK20), carcinoembryonic antigen (CEA), guanylyl cyclase C (GCC), epidermal growth
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Investigations for a multi-marker RT-PCR to improve sensitivity of disseminated tumor cell detection
NARCIS (Netherlands)
Vlems, F. A.; Diepstra, J. H. S.; Cornelissen, I. M. H. A.; Ligtenberg, M. J. L.; Wobbes, Th; Punt, C. J. A.; van Krieken, J. H. J. M.; Ruers, T. J. M.; van Muijen, G. N. P.
2003-01-01
In order to develop a multi-marker RT-PCR, which as such may be more sensitive than a single marker assay for the detection of disseminated tumor cells, we evaluated six RT-PCR markers: cytokeratin 20 (CK20), carcinoembryonic antigen (CEA), guanylyl cyclase C (GCC), epidermal growth factor receptor
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Comportamiento mecánico del polimetilmetacrilato sometido a envejecimiento artificial mediante cámara xenotest
Directory of Open Access Journals (Sweden)
Colom, X.
2001-04-01
Full Text Available In this investigation, the mechanical behavior of poly(methylmethacrylate due to aging in Xenon test chamber, using a xenon-arch lamp as a light radiation to simulate natural sun light has been studied. This work studies the mechanical properties (elongation at break. Young's modulus, tensile strength and toughness of PMMA samples exposed to different aging conditions (until 1560 h xenotest that correspond to 750 days exposure at natural light. The evolution of different mechanical properties characterizes the PMMA's process of degradation.
En el presente trabajo de investigación se ha estudiado el comportamiento mecánico del polimetilmetacrilato sometido a envejecimiento en cámara ''xenotest". El Xenotest es un aparato formado por una lámpara de arco de xenón que irradia en el intervalo 700-300 nm, simulando, adecuadamente, las condiciones de exposición a la luz solar. El estudio se centra en la caracterización de las propiedades mecánicas (módulo de Young, resistencia máxima a la tracción, deformación en el punto de rotura y tenacidad de las muestras de PMMA sometidas a las condiciones de envejecimiento durante períodos de tiempo variables (hasta 1.560 h xenotest que corresponden a una iluminación solar a la intemperie de 750 días. La evolución de las distintas propiedades mecánicas caracteriza el proceso de envejecimiento/degradación del PMMA.
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Free cancer cell detection in peritoneal cavity in gastric cancer patients by RT-PCR for CEA
Energy Technology Data Exchange (ETDEWEB)
Lee, Jong Inn; Moon, Nan Mo; Paik, Nam Sun; Choi, Dong Wook; Bang, Ho Yun; Hong, Seok Il [Korea Cancer Center Hospital, Seoul (Korea, Republic of)
1997-12-01
Authors applied RT-PCR assay to detecting CEA expressing free cancer cells in peritoneal cavity of 114 gastric cancer patients to find an indication for prophylactic treatment to prevent peritoneal recurrence. Sixty-three of 114 cases were positive for RT-PCR, of which 16 cases were positive for cytologic examination and 47 cases were negative. Forty-nine of 51 cases who were negative for RT-PCR were negative for cytologic examination. Positivity for RT-PCR according to the depth of invasion were as follows : two (28.6 %) of seven cases whose cancer invaded mucosal or submucosal layer were positive. Ten (45.5 %) of 22 cases whose cancer invaded muscular or subserosal layer were positive. Forty-one (57.7 %) of 71 serosa involved cases were positive. Eleven (78.6 %) of cases who had grossly perioneal seedings were positive (p=0.026). However, all of 7 EGC cases, 19 of 22 cases whose cancer invaded to muscle layer or to subserosa were negative for cytologic examination, and eight of 13 cases who had had peritoneal seedings were positive. Positivity for RT-PCR according to cell differentiation were as follows: forty-two (61.8 %) of 68 cases who cancer were poorly differentiated type were positive. (p=0.163) Serum level of CEA of RT-PCR positive group and that of negative group were not statistically different. It was revealed that RT-PCR was more sensitive than cytologic examination in detecting free tumor cells, especially in pm, ss and serosa positive cancers, so if further study with more cases and longer follow-up is performed, its role as prognostic factor and an indication of prophylactic therapy will be clarified. (author). 22 refs., 5 tabs.
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Free cancer cell detection in peritoneal cavity in gastric cancer patients by RT-PCR for CEA
International Nuclear Information System (INIS)
Lee, Jong Inn; Moon, Nan Mo; Paik, Nam Sun; Choi, Dong Wook; Bang, Ho Yun; Hong, Seok Il
1997-12-01
Authors applied RT-PCR assay to detecting CEA expressing free cancer cells in peritoneal cavity of 114 gastric cancer patients to find an indication for prophylactic treatment to prevent peritoneal recurrence. Sixty-three of 114 cases were positive for RT-PCR, of which 16 cases were positive for cytologic examination and 47 cases were negative. Forty-nine of 51 cases who were negative for RT-PCR were negative for cytologic examination. Positivity for RT-PCR according to the depth of invasion were as follows : two (28.6 %) of seven cases whose cancer invaded mucosal or submucosal layer were positive. Ten (45.5 %) of 22 cases whose cancer invaded muscular or subserosal layer were positive. Forty-one (57.7 %) of 71 serosa involved cases were positive. Eleven (78.6 %) of cases who had grossly perioneal seedings were positive (p=0.026). However, all of 7 EGC cases, 19 of 22 cases whose cancer invaded to muscle layer or to subserosa were negative for cytologic examination, and eight of 13 cases who had had peritoneal seedings were positive. Positivity for RT-PCR according to cell differentiation were as follows: forty-two (61.8 %) of 68 cases who cancer were poorly differentiated type were positive. (p=0.163) Serum level of CEA of RT-PCR positive group and that of negative group were not statistically different. It was revealed that RT-PCR was more sensitive than cytologic examination in detecting free tumor cells, especially in pm, ss and serosa positive cancers, so if further study with more cases and longer follow-up is performed, its role as prognostic factor and an indication of prophylactic therapy will be clarified. (author). 22 refs., 5 tabs
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Efeito agudo da intensidade do exercício de força na modulação autonômica cardíaca pós-exercício Efecto agudo de intensidad del ejercicio de fuerza en la modulación autónoma del corazón post-ejercicio Acute effect of resistance exercise intensity in cardiac autonomic modulation after exercise
Directory of Open Access Journals (Sweden)
Aluísio Henrique Rodrigues de Andrade Lima
2011-06-01
Full Text Available FUNDAMENTO: O balanço simpatovagal cardíaco se altera após o exercício de força. Contudo, o impacto das características do treinamento de força nessa resposta ainda não está claro. OBJETIVO: Analisar o efeito agudo da intensidade do exercício de força para tronco e membros superiores na modulação autonômica cardíaca pós-exercício. MÉTODOS: Quinze homens jovens realizaram três sessões experimentais em ordem aleatória: controle (C, exercício de força com 50% de 1-RM (E50% e exercício de força com 70% de 1-RM (E70%. As sessões incluíram 05 exercícios para tronco e membro superior, realizados em três séries de 12, 9 e 6 repetições, respectivamente. Antes e aos 20 e 50 minutos após as intervenções, a frequência cardíaca foi medida para posterior análise espectral da sua variabilidade. RESULTADOS: Em comparação aos valores anteriores à intervenção, o intervalo RR e a banda de alta frequência (AF aumentaram (maiores alterações: + 112 ± 83 ms; +10 ± 11 un, respectivamente, p 0,05. Em comparação aos valores pré-exercício, o intervalo RR e a banda AF diminuíram (maiores alterações: -69 ± 105 ms; -13 ± 14 un, respectivamente, p FUNDAMENTO: El balance simpatovogal cardíaco se altera después del ejercicio de fuerza. Sin embargo, el impacto de las características del entrenamiento de fuerza en esta respuesta no está claro. OBJETIVO: Analizar el efecto agudo de intensidad del ejercicio de fuerza para el tronco y miembros en la modulación autonómica del corazón post-ejercicio. MÉTODOS: Quince hombres jóvenes realizaron tres sesiones experimentales en orden aleatorio: control (C, ejercicio de fuerza con el 50% de 1-RM (E50% y ejercicio de fuerza con el 70% de 1-RM (E70%. Las sesiones incluyeron 05 ejercicios para tronco y miembro superior, realizados en tres series de 12, 9 y 6 repeticiones, respectivamente. Antes y a los 20 y 50 minutos después de las intervenciones, la frecuencia cardiaca se
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SPoRT Participation in the GOES-R and JPSS Proving Grounds
Science.gov (United States)
Jedlovec, Gary; Fuell, Kevin; Smith, Matthew
2013-01-01
For the last several years, the NASA Short-term Prediction Research and Transition (SPoRT) project at has been working with the various algorithm working groups and science teams to demonstrate the utility of future operational sensors for GOES-R and the suite of instruments for the JPSS observing platforms. For GOES-R, imagery and products have been developed from polar-orbiting sensors such as MODIS and geostationary observations from SEVIRI, simulated imagery, enhanced products derived from existing GOES satellites, and data from ground-based observing systems to generate pseudo or proxy products for the ABI and GLM instruments. The suite of products include GOES-POES basic and RGB hybrid imagery, total lightning flash products, quantitative precipitation estimates, and convective initiation products. SPoRT is using imagery and products from VIIRS, CrIS, ATMS, and OMPS to show the utility of data and products from their operational counterparts on JPSS. The products include VIIRS imagery in swath form, the GOES-POES hybrid, a suite of RGB products including the air mass RGB using water vapor and ozone channels from CrIS, and several DNB products. Over a dozen SPoRT collaborative WFOs and several National Centers are involved in an intensive evaluation of the operational utility of these products.
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A MIV-150/zinc acetate gel inhibits SHIV-RT infection in macaque vaginal explants.
Science.gov (United States)
Barnable, Patrick; Calenda, Giulia; Ouattara, Louise; Gettie, Agegnehu; Blanchard, James; Jean-Pierre, Ninochka; Kizima, Larisa; Rodríguez, Aixa; Abraham, Ciby; Menon, Radhika; Seidor, Samantha; Cooney, Michael L; Roberts, Kevin D; Sperling, Rhoda; Piatak, Michael; Lifson, Jeffrey D; Fernandez-Romero, Jose A; Zydowsky, Thomas M; Robbiani, Melissa; Teleshova, Natalia
2014-01-01
To extend our observations that single or repeated application of a gel containing the NNRTI MIV-150 (M) and zinc acetate dihydrate (ZA) in carrageenan (CG) (MZC) inhibits vaginal transmission of simian/human immunodeficiency virus (SHIV)-RT in macaques, we evaluated safety and anti-SHIV-RT activity of MZC and related gel formulations ex vivo in macaque mucosal explants. In addition, safety was further evaluated in human ectocervical explants. The gels did not induce mucosal toxicity. A single ex vivo exposure to diluted MZC (1∶30, 1∶100) and MC (1∶30, the only dilution tested), but not to ZC gel, up to 4 days prior to viral challenge, significantly inhibited SHIV-RT infection in macaque vaginal mucosa. MZC's activity was not affected by seminal plasma. The antiviral activity of unformulated MIV-150 was not enhanced in the presence of ZA, suggesting that the antiviral activity of MZC was mediated predominantly by MIV-150. In vivo administration of MZC and CG significantly inhibited ex vivo SHIV-RT infection (51-62% inhibition relative to baselines) of vaginal (but not cervical) mucosa collected 24 h post last gel exposure, indicating barrier effect of CG. Although the inhibitory effect of MZC (65-74%) did not significantly differ from CG (32-45%), it was within the range of protection (∼75%) against vaginal SHIV-RT challenge 24 h after gel dosing. Overall, the data suggest that evaluation of candidate microbicides in macaque explants can inform macaque efficacy and clinical studies design. The data support advancing MZC gel for clinical evaluation.
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A MIV-150/zinc acetate gel inhibits SHIV-RT infection in macaque vaginal explants.
Directory of Open Access Journals (Sweden)
Patrick Barnable
Full Text Available To extend our observations that single or repeated application of a gel containing the NNRTI MIV-150 (M and zinc acetate dihydrate (ZA in carrageenan (CG (MZC inhibits vaginal transmission of simian/human immunodeficiency virus (SHIV-RT in macaques, we evaluated safety and anti-SHIV-RT activity of MZC and related gel formulations ex vivo in macaque mucosal explants. In addition, safety was further evaluated in human ectocervical explants. The gels did not induce mucosal toxicity. A single ex vivo exposure to diluted MZC (1∶30, 1∶100 and MC (1∶30, the only dilution tested, but not to ZC gel, up to 4 days prior to viral challenge, significantly inhibited SHIV-RT infection in macaque vaginal mucosa. MZC's activity was not affected by seminal plasma. The antiviral activity of unformulated MIV-150 was not enhanced in the presence of ZA, suggesting that the antiviral activity of MZC was mediated predominantly by MIV-150. In vivo administration of MZC and CG significantly inhibited ex vivo SHIV-RT infection (51-62% inhibition relative to baselines of vaginal (but not cervical mucosa collected 24 h post last gel exposure, indicating barrier effect of CG. Although the inhibitory effect of MZC (65-74% did not significantly differ from CG (32-45%, it was within the range of protection (∼75% against vaginal SHIV-RT challenge 24 h after gel dosing. Overall, the data suggest that evaluation of candidate microbicides in macaque explants can inform macaque efficacy and clinical studies design. The data support advancing MZC gel for clinical evaluation.
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Dose preparatória de atracúrio: aferição da fadiga no músculo orbicular do olho e condições de intubação orotraqueal Dosis preparatoria de atracurio: comparación de la fatiga en el músculo orbicular del ojo y condiciones de intubación orotraqueal Priming dose of atracurium: measuring orbicularis oculi muscle fade and tracheal intubation conditions
Directory of Open Access Journals (Sweden)
Giovani de Figueiredo Locks
2003-12-01
clinicamente aceitáveis (p > 0,05. CONCLUSÕES: A dose preparatória de atracúrio (0,02 mg.kg-1 não causa fadiga no músculo orbicular do olho e não há diferença entre aguardar 5 ou 7 minutos como intervalo entre as doses do bloqueador neuromuscular.JUSTIFICATIVA Y OBJETIVOS: El método de la dosis preparatoria (priming principle consiste en la inyección de una pequeña dosis del bloqueador neuromuscular minutos antes de la administración de lo que queda de la dosis preconizada para intubación traqueal para encortar el inicio de acción de los bloqueadores neuromusculares (BNM adespolarizantes. Existe, sin embargo, posibilidad de fatiga muscular y broncoaspiración. De especial interés para las maniobras de intubación orotraqueal y protección de las vías aéreas, son los músculos de la laringe. Como su monitorización directa impone dificultades técnicas, se demostró que el músculo orbicular del ojo presenta correlación con los músculos laríngeos cuanto a la sensibilidad a los BNM. El objetivo de esta pesquisa es evaluar si hay fatiga del músculo orbicular ocular después del uso de la dosis preparatoria de atracúrio y comparar las condiciones clínicas de intubación traqueal después de dos intervalos entre las dosis. MÉTODO: Fueron seleccionados 35 pacientes adultos, estado físico ASA I o II, sin factores de riesgo para broncoaspiración, sometidos a cirugía electiva. Fue inducida anestesia general con alfentanil y propofol y el paciente fue ventilado manualmente bajo máscara. En seguida, fueron posicionados los electrodos para estimular el ramo temporal del nervio facial y el transductor de aceleración en el músculo orbicular del ojo. Se administró dosis preparatoria de atracúrio (0,02 mg.kg-1 y la relación T4/T1 fue evaluada a cada minuto durante 5 minutos en 20 casos (G1 y 7 minutos en 13 casos (G2. Después de este intervalo, se administró la dosis complementar de atracúrio (0,5 mg.kg-1 y se procedió a la intubación orotraqueal despu
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Prevalence and correlates of preterm labor among young parturient women attending public hospitals in Brazil Prevalencia y factores correlativos del parto prematuro en las jóvenes parturientas que acuden a los hospitales públicos del Brasil
Directory of Open Access Journals (Sweden)
Angélica E. Miranda
2012-11-01
Full Text Available OBJECTIVE: To determine the prevalence of and risk factors for preterm labor (OBJETIVO: Determinar la prevalencia y los factores de riesgo de parto prematuro (menos de 37 semanas de gestación en las jóvenes embarazadas del Brasil. MÉTODOS: Se llevó a cabo un estudio transversal a escala nacional en jóvenes parturientas de 15 a 24 años de edad que acudieron a las maternidades públicas brasileñas en el 2009. Los entrevistadores administraron cuestionarios al ingresar la parturienta en la maternidad (o poco después de parto, si las mujeres participantes tenían demasiado dolor como para responder durante el trabajo del parto. Las participantes respondieron a un cuestionario que recopilaba datos demográficos, conductuales y clínicos. RESULTADOS: Se incluyeron en el estudio un total de 2 071 (86,3% parturientas de las 2 400 invitadas a participar. La media de edad fue de 20,2 años (desviación estándar [SD]: 2,7 y el promedio de años de enseñanza escolar fue de 8 (SD: 2,4. Se produjo un parto prematuro en 450 mujeres (21,7%. La proporción más alta de parto prematuro (36,1% se observó en la región del Norte y la proporción más baja (6,9% en la región del Sur. En el modelo final de regresión logística con variables múltiples, el vivir en la región del Norte (razón de posibilidades [OR]: 1,43; intervalo de confianza [IC] de 95%: 1,28-1,59; el tener ingresos mensuales inferiores a dos veces el salario mínimo brasileño (OR: 1,27, IC de 95%: 1,01-1,60; el haber sido víctima de violencia doméstica (OR: 2,27, IC de 95%: 1,23-4,18; y el haber recibido una atención prenatal inadecuada (OR: 3,17, IC de 95%: 2,54-3,97 seguían siendo factores correlativos significativos de parto prematuro en esta población. CONCLUSIONES: Los resultados muestran una alta prevalencia del parto prematuro en las mujeres jóvenes del Brasil. El parto prematuro presenta una etiología multifactorial. Se deben destinar más recursos de salud p
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Validación del Código Rojo: una propuesta para el tratamiento de la hemorragia obstétrica
Directory of Open Access Journals (Sweden)
Gladis Adriana Vélez Álvarez
2013-10-01
Full Text Available OBJETIVO: Evaluar el efecto del entrenamiento en una guía de manejo, con base en escenarios de simulación, sobre la prevención y el tratamiento de la hemorragia obstétrica. MÉTODOS: Se realizó un estudio cuasi experimental de medición antes y después. La intervención consistió en dos jornadas -con diferencia de seis meses- de capacitación en escenarios de simulación en el reconocimiento y el tratamiento de la hemorragia obstétrica y la entrega de recordatorios con la estrategia. Se evaluó el efecto sobre el manejo de las pacientes mediante la revisión de las historias clínicas 24 meses antes y 18 meses después de la intervención. RESULTADOS: En los 445 miembros del personal de salud capacitados, la mediana de la calificación pasó de 55% de respuestas correctas -antes de la capacitación- a 85% después (P < 0,001. Se presentaron 124 (2,1% casos de hemorragia antes y 86 (1,8% después de la intervención, con una disminución de la incidencia de 16,5% (riesgo relativo [RR] 0,84; intervalo de confianza de 95% [IC95%]: 0,6-1,1. El empleo de la oxitocina aumentó de 58,3% a 74,1% (RR: 1,3; IC95%: 1,0-1,6 y la vigilancia activa del puerperio inmediato pasó de 5% a 36,5% (RR: 7,4; IC95%: 3,0-18,1. CONCLUSIONES: La intervención realizada incrementó el uso de algunas prácticas beneficiosas para la prevención y detección temprana de la hemorragia, y obtuvo una mejoría no estadísticamente significativa en la incidencia de hemorragia. Es necesario implementar entrenamientos de simulación de una manera periódica, acompañados de otras estrategias ajustadas a la realidad local y a las circunstancias del grupo.
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Preoperative radiotherapy (RT) for prevention of heterotopic ossification (HO) after total hip replacement
International Nuclear Information System (INIS)
Heyd, R.; Schopohl, B.; Boettcher, H.D.; Kirchner, J.
1997-01-01
Preliminary results of a prospective study which investigates the efficacy of preoperative radiotherapy (RT) for prevention of heterotopic ossification (HO) after total hip arthroplasty are summarized. A total number of 20 hip joints (18 patients) were irradiated with a single dose of 6.0 Gy Brooker grade II). The functional outcome quantified with the Harris score was improved by an average of 37.9 points. The authors conclude that preoperative RT is an effective alternative for postoperative irradiation. (orig.) [de
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Pärt, Arvo: De profundis. Missa Sillabica / Patric Wiklacz
Index Scriptorium Estoniae
Wiklacz, Patric
1997-01-01
Uuest heliplaadist "Pärt, Arvo: De profundis. Missa Sillabica. Solfeggio. And one of the Pharisees. Cantate Domino. Summa. 7 Antiennes du Magnificat. The Beautitudes. Magnificat. Christopher Bowers-Broadbent (org.), Daniel Kennedy (perc.). Theatre of Voices / Paul Hillier (bass)" Harmonia Mundi HMU907 182 F (CD:165F). 1996. TT:1h 16'06"
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A realidade do uso da monitorização neurofisiológica intraoperatória entre os cirurgiões de coluna brasileiros La realidad del uso del monitoreo neurofisiológico intraoperatorio entre los cirujanos de columna brasileños The reality of using intraoperative neurophysiologic monitoring among Brazilian spine surgeons
Directory of Open Access Journals (Sweden)
Martins Back Netto
2012-12-01
Full Text Available OBJETIVO: Avaliar o uso da monitorização neurofisiológica intraoperatória (MNIO por cirurgiões de coluna brasileiros. MÉTODO: A coleta de dados foi realizada através de um questionário aplicado em 307 cirurgiões de coluna brasileiros, durante o 11º Congresso de Cirurgia Espinhal e XIII Congresso da Sociedade Brasileira de Coluna. RESULTADOS: Dos cirurgiões entrevistados, 42% são neurocirurgiões e 58% ortopedistas. A maioria (72,3% relatou que já fez uso do MNIO, entretanto apenas 29,6% utilizam este procedimento rotineiramente. Destes 39% são ortopedistas. Entre os neurocirurgiões, a maior parte (84% relatou não utilizar MNIO como rotina. Nos casos de deformidade, 85,7% dos profissionais disseram usar rotineiramente a MNIO. Do total, 68,1% responderam que não tinham fácil acesso à MNIO, sendo que 10% deles atuam na região Centro-oeste do país e 11%, na região Nordeste. Dos que relataram facilidade de acesso ao procedimento, 77% atuam na região Sudeste. A média de idade dos participantes foi 41,9 anos, com mediana de 39,0, desvio padrão de 11,3 e intervalo de confiança de 1,3. Para o tempo de formação médica, a média foi 17,8 anos, com mediana de 14,0 e intervalo de confiança 1,2. Em relação ao tempo de prática em cirurgia de coluna encontrou-se que 56,3% têm até 10 anos de prática. CONCLUSÃO: A maioria dos cirurgiões de coluna já fez uso da MNIO, contudo poucos utilizam-na como rotina. A região Sudeste é onde se encontra maior facilidade de acesso à MNIO, ao contrário das regiões Centro-oeste e Nordeste.OBJETIVO: Evaluar el uso del monitoreo neurofisiológico intraoperatorio (MNIO por cirujanos de columna brasileños. MÉTODO: La recolección de datos fue realizada a través de un cuestionario aplicado en 307 cirujanos de columna brasileños, durante el 11º Congresso de Cirurgia Espinhal y el XIII Congresso da Sociedade Brasileira de Coluna. RESULTADOS: De los cirujanos entrevistados, el 42 % son
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EL ESTÍMULO DEL MACHO CABRÍO INCREMENTA LA FUNCIÓN REPRODUCTIVA DE LAS CABRAS CRIOLLAS DEL SEMIDESIERTO MEXICANO INDEPENDIENTEMENTE DEL RÉGIMEN DE FOTOPERIODO
Directory of Open Access Journals (Sweden)
Romana Melba Rincón-Delgado
2011-01-01
Full Text Available Se evaluó la influencia de un macho cabrío sexualmente activo sobre el inicio de la actividad reproductiva (actividad hipotalámica y ovárica y la sucesión de los ciclos estrales en cabras criollas expuestas a fotoperiodo artificiales alternados (decreciente/creciente en un rango de 13.4 a 10.6 horas de luz por día hasta completar 6 ciclos fotoperiódicos de 90 d por cada ciclo: fotoperiodo creciente (n=3; fotoperiodo decreciente (n=3. Las cabras criollas procedentes del semidesierto zacatecano (n=30 fueron asignadas de manera aleatoria a dos grupos: 1 cabras expuestas a un macho cabrío sexualmente activo (n=15; 2 cabras no expuestas al macho cabrío (ausencia de macho cabrío; n=15. Dentro de cada grupo experimental existían cabras ovariectomizadas (OVX, n=5, cabras ovariectomizadas e implantadas con estradiol (OVX + E2, n=5, y cabras ovariointactas (testigo, n=5. Se recolectaron muestras de suero sanguíneo de las cabras OVX y OVX + E2, cada cuatro semanas, durante 6 h en intervalos de 15 min (es decir, 24 muestras/día, para determinar la frecuencia (FREQ, la amplitud (AMP y la concentración (CONC de la hormona luteinizante (HL. En las cabras testigo (no tratadas, se obtuvieron 2 muestras de sangre a la semana para cuantificar los niveles de progesterona en suero sanguíneo mediante un radioinmunoanálisis (RIA. Las cabras con implantes de E2 (cabras OVX + E2 mostraron un incremento en la frecuencia de los pulsos de la hormona luteinizante en comparación con las cabras OVX sin implante de E2 (2.0±0.5 vs.0.7 ± 0.1 HL pulsos/6 h. La presencia de un macho sexualmente activo aumenta la frecuencia, la amplitud y la concentración de la hormona luteinizante en cabras OVX en comparación con cabras no expuestas a machos (Frecuencia: 3.2 ± 0.4 vs. 0.7 ± 0.1 pulsos/6 h; Amplitud: 1.6 ± 0.1 vs. 0.8 ± 0.3 ng·mL-1; Concentración 5.3 ± 0.6 vs. 2.0 ± 0.9 ng·mL-1 (P0.05. En conclusión, la presencia de un macho cabrío sexualmente activo
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Rooma põleb, kirjanik mängib viiulit / Kärt Hellerma
Index Scriptorium Estoniae
Hellerma, Kärt, 1956-
1996-01-01
Arvustus: Orwell, George. Valaskala kõhus. Tln.: Vagabund, 1995. Ilmunud ka kogumikus: Hellerma, Kärt. Kohanenud kirjandus : valik kirjanduskriitikat 1987-2006. Eesti Keele Sihtasutus : Tallinn, 2006. Lk. 173-175
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The effect of reverse triiodothyronine (rT3) treatment on the plasma levels of iodothyronines in growing Japanese quails
International Nuclear Information System (INIS)
Niezgoda, J.; Wronska-Fortuna, D.; Sechman, A.; Bobek, S.; Sowa, J.
1994-01-01
It has been established that 3.3',5-triiodothyronine (T 3 ) acts hipermetabolically, whereas 3'3.5'-triiodothyronine (reverse T 3 , rT 3 ) acts hypometabolically. The normal ratio of T 3 to rT 3 in domestic flows fluctuates around 10. Since the excess of T 3 may promote catabolic processes and lower weight gain, the aim of this experiment was to find the optimal ratio of T 3 :rT 3 in blood plasma which corresponds with the greatest weight gain in Japanese quail. Reverse T 3 was given in drinking water (1 μg rT 3 /ml) for 3, 10, 20 or 30 days. It has been found that T 3 /rT 3 ratio of about 5, after 20 days of rT 3 administration, stimulated the weight gain of Japanese quail of both sexes from 7 to 44% with more pronounced effect in female birds. (author). 26 refs, 4 figs, 1 tab
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Cardiomiopatía inducida por estrés (Takotsubo en una paciente con anorexia nerviosa
Directory of Open Access Journals (Sweden)
Sabrina Vadalá
2014-06-01
Full Text Available Presentamos el caso de una mujer con diagnóstico de anorexia nerviosa que desarrolló cardiomiopatía de takotsubo precipitada por estrés emocional y alteraciones del medio interno. Evolucionó favorablemente con manejo conservador. Los casos de cardiomiopatía inducida por estrés, descriptos en pacientes con trastornos de la conducta alimentaria, suelen alcanzar mayor gravedad y se asocian con la prolongación del intervalo QT por desequilibrios electrolíticos, arritmias ventriculares e hipoglucemia. Se realiza una revisión del compromiso cardiovascular en pacientes con anorexia nerviosa.
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Factores que influyen en la emesis postaturdimiento en bovinos
OpenAIRE
Francisco Gerardo Ríos Rincón; Alfredo Estrada Angulo; Jorge Hernández Bautista; Cristina Pérez Linares; Jesús José Portillo Loera; Juan Carlos Robles Estrada
2012-01-01
Para determinar el efecto del tiempo de espera pre-sacrificio, la efectividad del aturdimiento, el tipo de categoría comercial y la consistencia del contenido ruminal en la emesis durante el intervalo entre aturdimiento y desangrado en ganado bovino, se seleccionaron 9,446 canales en cuatro plantas procesadoras (P1, P2, P3, P4). El tiempo de espera pre-sacrificio se clasificó en: 1) menor a 3 h (T1); 2) de 3 a 12 h (T2); 3) mayor a 12 h (T3). El aturdimiento se registró como efectivo y no efe...
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Factores que influyen en la emesis post-aturdimiento en bovinos
OpenAIRE
Ríos Rincón, Francisco Gerardo; Estrada Angulo, Alfredo; Hernández Bautista, Jorge; Pérez Linares, Cristina; Portillo Loera, Jesús José; Robles Estrada, Juan Carlos
2012-01-01
Para determinar el efecto del tiempo de espera pre-sacrificio, la efectividad del aturdimiento, el tipo de categoría comercial y la consistencia del contenido ruminal en la emesis durante el intervalo entre aturdimiento y desangrado en ganado bovino, se seleccionaron 9,446 canales en cuatro plantas procesadoras (P1, P2, P3, P4). El tiempo de espera pre-sacrificio se clasificó en: 1) menor a 3 h (T1); 2) de 3 a 12 h (T2); 3) mayor a 12 h (T3). El aturdimiento se registró como efectivo y no efe...
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Factores que influyen en la emesis postaturdimiento en bovinos
Directory of Open Access Journals (Sweden)
Francisco Gerardo Ríos Rincón
2012-01-01
Full Text Available Para determinar el efecto del tiempo de espera pre-sacrificio, la efectividad del aturdimiento, el tipo de categoría comercial y la consistencia del contenido ruminal en la emesis durante el intervalo entre aturdimiento y desangrado en ganado bovino, se seleccionaron 9,446 canales en cuatro plantas procesadoras (P1, P2, P3, P4. El tiempo de espera pre-sacrificio se clasificó en: 1 menor a 3 h (T1; 2 de 3 a 12 h (T2; 3 mayor a 12 h (T3. El aturdimiento se registró como efectivo y no efectivo. La consistencia del contenido ruminal, se clasificó en: acuosa, semi-acuosa y espesa. En P1, se registró el número de cabezas y pulmones contaminados por emesis, y el análisis se realizó mediante X2. Cuando la espera pre-sacrificio fue mayor a 10 h y el aturdimiento no fue efectivo, el riesgo fue 1.73 veces mayor para observar contaminación de la canal. Al aumentar el tiempo de espera pre-sacrificio se incrementó (P<0.05 la emesis, cuando el contenido ruminal era acuoso (62.9 vs 12.5 y 24.6 %. La emesis fue similar (P=0.10; 10.07 % entre machos y hembras. El tiempo de espera pre-sacrificio y la categoría comercial del ganado no influyeron en la emesis post-aturdimiento. Se concluye que el efecto combinado de mayor tiempo de espera pre-sacrificio, aturdimiento no efectivo y consistencia acuosa del contenido ruminal, favorecen la emesis en bovinos durante el intervalo del aturdimiento al desangrado.
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DINÃMICA DE LA RESPUESTA HUMORAL ESPECÃFICA Y FRECUENCIA DE VARIANTES DEL VIRUS DE PRRS EN TRES GRANJAS PORCINAS CON DIFERENTES ESTRATEGIAS DE CONTROL, EN YUCATÃN, MÉXICO
Directory of Open Access Journals (Sweden)
Pilar Gómez-Ruiz
2014-04-01
Full Text Available El objetivo de este estudio fue describir la dinámica de la respuesta humoral especÃfica y la frecuencia de variantes del virus del PRRS en tres granjas con diferentes estrategias de control. Treinta cerdos en cada granja fueron estudiados. Los animales se muestrearon y los sueros se procesaron mediante ELISA y RT-PCR in tiempo real usando tecnologÃa Sybr Green para detectar las variantes del virus del PRRS. La media de los valores de S/P, RT-PCR y temperatura de desnaturalización se compararon a los 21, 49, 77,105 y 160 dÃas de edad. Los cerdos de las granja A fueron positivos a la prueba de ELISA en todas las edades; en la granja B los cerdos fueron positivos a los 77, 105 y 160 dÃas de edad, y los cerdos de la granja C fueron positivos a los 105 y 160 dÃas de edad. Las temperaturas de desnaturalización, mostraron cuatro diferentes tiempos de desnaturalización en cada granja, correspondiendo a cuatro tipos de virus: virus vacunal, inóculo y dos tipos silvestres de virus. En conclusión, las diferentes estrategias utilizadas en las granjas indujeron una respuesta inmune en la mayorÃa de los animales. En la granja A se obtuvieron las medias más altas de S/P. Las estrategias de control no evitaron la presencia de variantes del virus del PRRS por lo que pudieran representar un riesgo para la población animal.
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Estructura espacial de las órbitas caóticas en un modelo autoconsistente de galaxia elíptica
Science.gov (United States)
Muzzio, J. C.
Hemos logrado construir modelos autoconsistentes de sistemas estelares utilizando una aproximación cuadrupolar para el potencial. Esto nos permite determinar órbitas y exponentes de Lyapunov de objetos que tienen posiciones y velocidades equivalentes a las que se obtienen de la funcón de distribución del sistema. La distribución espacial de las órbitas caóticas exhibe considerable estructura y, lo que es más importante aún, los valores de los exponentes de Lyapunov calculados sobre intervalos finitos de tiempo, muestran una fuerte correlación con el comportamiento de la órbita en esos mismos intervalos, por lo que permiten reconocer distintos subsistemas con diferentes distribuciones espaciales.
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L’uso della vegetazione per il controllo del rumore - The use of vegetation for noise control
Directory of Open Access Journals (Sweden)
Jian Kang
2015-09-01
Full Text Available Questo articolo riassume agli aspetti acustici di base della vegetazione nella sua applicazione in contesti urbani. È stato riscontrato che i massimi coefficienti di assorbimento acustico e di scattering sono rispettivamente, 0.49 a 5 kHz e 0,43 a 2.5 kHz. Per gli alberi, i risultati mostrano che tempi di riverberazione proporzionali alle dimensioni della chioma. Il massimo tempo di riverbero RT20 misurato è stato di 0,28 s a 4 kHz. Anche tetti/superfici verdi posizionati ai lati della strada sono risultati essere efficaci per l'attenuazione del suono. Simulazioni al computer con l’utilizzo di questi sistemi, per ridurre il rumore del traffico stradale, in piazze e cortili, sono presentate. -------- This paper reviews the basic acoustic characteristics of vegetation relating to its application in urban context. It was found that the maximum acoustic absorption and scattering coefficients of aboveground vegetation studied here was 0.49 at 5000 Hz and 0.43 at 2500 Hz, respectively. For trees, the results show that the reverberation time is proportional to the tree crown size, which is the most important factor. The maximum reverberation time RT20 measured was 0.28 s at 4000 Hz. Low profile green roof systems on road sides are shown to be effective in reducing sound. Computer simulations for reducing traffic noise in streets, squares and courtyards, have also been carried out.
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Factores contribuyentes al desarrollo de infecciones adquiridas en la comunidad en diabéticos tipo 2 admitidos en salas de medicina del Hospital Nacional Cayetano Heredia
Directory of Open Access Journals (Sweden)
Tapia-Zegarra Gino Guillermo
2001-01-01
Full Text Available El propósito del estudio es identificar factores contribuyentes al desarrollo de infecciones adquiridas en la comunidad en diabéticos tipo 2 hospitalizados. De tal forma, se siguió el diseño de un estudio de casos y controles no apareado. Consecuentemente, se eligieron en forma aleatoria 105 diabéticos con infección adquirida en la comunidad y 105 diabéticos con patología no infecciosa, obtenidos de los Servicios de Medicina de un hospital universitario, entre 1991 y 1998. Mediante el análisis bivariado y multivariado, se obtuvo la estimación ajustada del valor de odds ratio. Los resultados permitieron, según análisis estratificado por edad y sexo, obtener a neuropatía autonómica como factor contribuyente de infecciones urinarias (OR = 4,07. En el modelo multivariado se aisló a vasculopatía periférica (R²: 0,24 como contribuyente de infección de piel y partes blandas (OR = 6,79. Los intervalos de confianza fueron significativos y de 95% de confiabilidad. En conclusión, vasculopatía periférica y vejiga neurogénica contribuyen al desarrollo de infecciones adquiridas en la comunidad en diabéticos tipo 2 hospitalizados.
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Intelligent software systems and SMiRT: Potentials, actual results, expectations, trends
International Nuclear Information System (INIS)
Jovanovic, A.
1993-01-01
The paper gives a survey of recent development trends in the area of knowledge-based systems, hypermedia, neural networks and other similar technologies which are on the baseline of modern 'intelligent' software systems, applied in the areas relevant for SMiRT: power plant operation, design and analysis of structural components, materials, and many others. The paper highlights the historical background of these trends, as well as the methodologies and technologies which made such a development possible ('enabling methodologies/technologies'). Examples from several, SMiRT characteristic application areas are mentioned, in order to give an illustration what the deployment of an intelligent software system can mean in practice. Finally, a summary of these result is made and future perspectives indicated. (author)
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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
Science.gov (United States)
EPA Method 1615 measures enteroviruses and noroviruses present in environmental and drinking waters. The viral ribonucleic acid (RNA) from water sample concentrates is extracted and tested for enterovirus and norovirus RNA using reverse transcription-quantitative PCR (RT-qPCR). V...
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DETECTION OF CLASSICAL SWINE FEVER VIRUS BY RT-PCR IN WEST BENGAL, INDIA
Directory of Open Access Journals (Sweden)
Sumit Chowdhury
2016-12-01
Full Text Available Classical swine fever is a deadly disease of swine, caused by a RNA virus. The present study has identified presence of the classical swine fever virus (CSFV in pigs of West Bengal by one step reverse transcriptase PCR (RT-PCR performed using 5’ NTR specific primers. Internal organs from clinically affected pigs were examined from three districts of West Bengal. RT-PCT has identified presence of CSFV in all the tissues examined confirming presence of CSFV in different parts of the state.
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The role of pleural fluid MAGE RT-nested PCR in the diagnosis of malignant pleural effusion.
Science.gov (United States)
Jeon, Eun Ju; Park, Hye Kyeong; Jeon, Kyeongman; Koh, Won-Jung; Suh, Gee Young; Chung, Man Pyo; Kim, Hojoong; Kwon, O Jung; Ki, Chang-Seok; Kim, Jong-Won; Shim, Young Mog; Um, Sang-Won
2012-11-01
Melanoma antigen (MAGE) genes are expressed in tumor cells, the testis and the placenta. The purpose of this prospective study was to investigate the sensitivity, specificity, and accuracy of the carcinoembryonic antigen (CEA), MAGE reverse transcriptase-nested polymerase chain reaction (RT-nested PCR), and cytology of pleural fluid in the diagnosis of malignant pleural effusion. Patients in whom unilateral pleural effusion was identified on chest radiography from January to December 2009 were included in the study. MAGE genes were analyzed by RT-nested PCR using MAGE A1-6 common primers. Of 81 enrolled patients, 46 were diagnosed as malignant pleural effusion, and 24 were diagnosed as benign pleural effusion. The diagnoses of 11 patients were not confirmed in this study. The diagnostic sensitivity, specificity, and accuracy of MAGE RT-nested PCR were 61.4%, 95.7%, and 73.1%, respectively. The diagnostic sensitivities of cytology and CEA (>5 ng/mL) were 61.4% and 75.0%, respectively. Among 17 patients with negative cytology who had malignant pleural effusion, 12 and 10 patients were positive for CEA (>5.0 ng/mL) and MAGE RT-nested PCR, respectively. However, of five patients with malignant pleural effusion that was not recognized by cytology and CEA, MAGE RT-nested PCR correctly predicted a malignant etiology in only one additional patient (20%). MAGE RT-nested PCR seems to add little on the combination of conventional methods in the diagnosis of malignant effusion. © 2012 Tianjin Lung Cancer Institute and Wiley Publishing Asia Pty. Ltd.
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Pärt: Fratres in sechs Versionen. Festina lente. Summa / C. S.
Index Scriptorium Estoniae
C. S.
1997-01-01
Uuest heliplaadist "Pärt: Fratres in sechs Versionen. Festina lente. Summa. Cantus in memoriam Benjamin Britten. Orchester der Ungarischen Staatsoper, Tamas Benedek". Naxos CD 8 553 750 (WD:79'06") DDD
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The development and application of the two real-time RT-PCR assays to detect the pathogen of HFMD.
Directory of Open Access Journals (Sweden)
Aili Cui
Full Text Available Large-scale Hand, Foot, and Mouth Disease (HFMD outbreaks have frequently occurred in China since 2008, affecting more than one million children and causing several hundred children deaths every year. The pathogens of HFMD are mainly human enteroviruses (HEVs. Among them, human enterovirus 71 (HEV71 and coxsackievirus A16 (CVA16 are the most common pathogens of HFMD. However, other HEVs could also cause HFMD. To rapidly detect HEV71 and CVA16, and ensure detection of all HEVs causing HFMD, two real-time hybridization probe-based RT-PCR assays were developed in this study. One is a multiplex real-time RT-PCR assay, which was developed to detect and differentiate HEV71 specifically from CVA16 directly from clinical specimens within 1-2 h, and the other is a broad-spectrum real-time RT-PCR assay, which targeted almost all HEVs. The experiments confirmed that the two assays have high sensitivity and specificity, and the sensitivity was up to 0.1 TCID50/ml for detection of HEVs, HEV71, and CVA16, respectively. A total of 213 clinical specimens were simultaneously detected by three kinds of assays, including the two real-time RT-PCR assays, direct conventional RT-PCR assay, and virus isolation assay on human rhabdomyosarcoma cells (RD cells. The total positive rate of both HEV71 and CVA16 was 69.48% with real-time RT-PCR assay, 47.42% with RT-PCR assay, and 34.58% with virus isolation assay. One HFMD clinical specimen was positive for HEV, but negative for HEV71 or CVA16, which was identified as Echovirus 11 (Echo11 by virus isolation, RT-PCR, and sequencing for the VP1 gene. The two real-time RT-PCR assays had been applied in 31 provincial HFMD labs to detect the pathogens of HFMD, which has contributed to the rapid identification of the pathogens in the early stages of HFMD outbreaks, and helped to clarify the etiologic agents of HFMD in China.
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Evaluación del desarrollo en vivero de Peltogyne purpurea Pittier (Nazareno
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Ramón Antonio Mosquera Mena
2015-12-01
Full Text Available La presente investigación se realizó teniendo en cuenta la importancia ecológica de la especie Peltogyne purpurea Pittier, ayudando a conservar la estructura del bosque natural, su importancia económica como especie de gran valor comercial, su situación de especie en vía de extinción y la posibilidad de regeneración por medio de semilla en vivero, con el objetivo de evaluar el comportamiento agronómico en vivero bajo cuatro sustratos diferentes en la finca Tulenapa ubicada en el municipio de Carepa (Antioquia, Colombia, la cual cuenta con las condiciones meteorológicas y medioambientales adecuadas para la adaptabilidad de la especie; se hace un análisis del comportamiento en vivero de esta plántula, evaluando la altura, el diámetro, el número de hojas y la supervivencia a los 90 días, utilizando cuatro sustratos bien definidos y de fácil consecución en la zona: gallinaza, codornaza, tierra con materia orgánica y un sustrato testigo compuesto por cascarilla de arroz y arena. El diseño estadístico empleado fue bloques compuestos al azar, se realizaron seis mediciones de las variables en estudio con intervalos de 15 días, a los resultados se les aplicó un análisis de varianza ANOVA y prueba P para análisis de medias. Se encontraron diferencias significativas para las variables diámetro y sobrevivencia. Las demás variables no presentan diferencias significativas. Al encontrar diferencia significativa en el tratamiento tierra para la sobrevivencia, se recomienda hacer la multiplicación de la especie en vivero utilizando el sustrato más parecido a su entorno natural, lo que facilitará dicho proceso a productores interesados.
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Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR.
Science.gov (United States)
Kuamsab, Napaporn; Putaporntip, Chaturong; Pattanawong, Urassaya; Jongwutiwes, Somchai
2012-06-10
Gametocyte carriage is essential for malaria transmission and endemicity of disease; thereby it is a target for malaria control strategies. Malaria-infected individuals may harbour gametocytes below the microscopic detection threshold that can be detected by reverse transcription polymerase chain reaction (RT-PCR) targeting gametocyte-specific mRNA. To date, RT-PCR has mainly been applied to the diagnosis of Plasmodium falciparum gametocytes but very limited for that of Plasmodium vivax. A multiplex-nested RT-PCR targeting Pfs25 and Pvs25 mRNA specific to mature gametocytes of P. falciparum and P. vivax, respectively, was developed. The assay was evaluated using blood samples collected in rainy and dry seasons from febrile patients,in a malaria-endemic area in Thailand. Malaria diagnosis was performed by Giemsa-stained blood smears and 18S rRNA PCR. The multiplex-nested RT-PCR detected Pfs25 mRNA in 75 of 86 (87.2%) P. falciparum-infected individuals and Pvs25 mRNA in 82 of 90 (91.1%) P. vivax malaria patients diagnosed by 18S rRNA PCR. Gametocytes were detected in 38 (eight P. falciparum and 30 P. vivax) of 157 microscopy positive samples, implying that a large number of patients harbour sub-microscopic gametocytaemia. No seasonal differences in gametocyte carriage were observed for both malaria species diagnosed by multiplex-nested RT-PCR. With single-nested RT-PCR targeting Pfs25 or Pvs25 mRNA as standard, the multiplex-nested RT-PCR offered sensitivities of 97.4% and 98.9% and specificities of 100% and 98.8% for diagnosing mature gametocytes of P. falciparum and P. vivax, respectively. The minimum detection limit of the multiplex-nested PCR was 10 copies of templates. The multiplex-nested RT-PCR developed herein is useful for simultaneous assessment of both P. falciparum and P. vivax gametocyte carriage that is prevalent and generally sympatric in several malaria-endemic areas outside Africa.
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Selection of suitable reference genes for RT-qPCR analyses in cyanobacteria.
Directory of Open Access Journals (Sweden)
Filipe Pinto
Full Text Available Cyanobacteria are a group of photosynthetic prokaryotes that have a diverse morphology, minimal nutritional requirements and metabolic plasticity that has made them attractive organisms to use in biotechnological applications. The use of these organisms as cell factories requires the knowledge of their physiology and metabolism at a systems level. For the quantification of gene transcripts real-time quantitative polymerase chain reaction (RT-qPCR is the standard technique. However, to obtain reliable RT-qPCR results the use and validation of reference genes is mandatory. Towards this goal we have selected and analyzed twelve candidate reference genes from three morphologically distinct cyanobacteria grown under routinely used laboratory conditions. The six genes exhibiting less variation in each organism were evaluated in terms of their expression stability using geNorm, NormFinder and BestKeeper. In addition, the minimum number of reference genes required for normalization was determined. Based on the three algorithms, we provide a list of genes for cyanobacterial RT-qPCR data normalization. To our knowledge, this is the first work on the validation of reference genes for cyanobacteria constituting a valuable starting point for future works.
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Fiabilidad del test 6 minutos caminando en personas con secuelas de poliomielitis paralítica mediante test-retest de 12 semanas
Directory of Open Access Journals (Sweden)
Francisco Javier Domínguez-Muñoz
2013-01-01
Full Text Available El análisis de la fiabilidad del test de 6 minutos ca- minando en una población de personas con secuelas de poliomielitis paralítica mediante test-retest de 12 semanas no ha sido estudiado. Participaron personas con secuelas de poliomielitis paralítica (n = 18; 48,72 ± 7,69 años; 65,8 ± 11,6 kg. Se les realizó un test-retest de 12 semanas de la prueba de 6 minutos caminando que consistía en que los sujetos anduvieran la mayor distan- cia, sin llegar a la carrera, en un periodo de 6 minutos. La fiabilidad relativa de la prueba fue excelente (CCI = 0,99. En lo que se refiere a la fiabilidad absoluta se obtuvo un error estándar de medida (SEM del 1,7% y un mínimo cambio real (SRD de 4,7%. La fiabilidad del test de 6 minutos caminando usando el método Bland- Altman mostró que el error sistemático (diferencia de medias entre el test-retest fue 2,72 (bias. En conclu- sión, los resultados obtenidos en el test de 6 minutos ca- minando han sido muy fiables y afirmamos que la prue- ba de 6 minutos caminando podrá ser utilizada como prueba de evaluación en una población con secuelas de poliomielitis paralítica, con un intervalo de 12 semanas entre las dos mediciones, para comprobar los cambios que se han producido tras la aplicación de un programa de actividad física.
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Use of alternative alkali chlorides in RT and PCR of polynucleotides containing G quadruplex structures.
Science.gov (United States)
Ramos-Alemán, Fabiola; González-Jasso, Eva; Pless, Reynaldo C
2018-02-15
Several alkali chlorides were compared for their use in reverse transcription (RT) and PCR of different types of nucleic acid templates. On a test region of biological DNA incapable of forming G quadruplex (G4) structures, Taq DNA polymerase showed similar PCR performance with 50 mM KCl, CsCl, LiCl, and NaCl. In contrast, on a synthetic model polydeoxyribonucleotide prone to G4 formation, good PCR amplification was obtained with 50 mM CsCl, but little or none with LiCl or KCl. Similarly, in RT of a G4-prone model polyribonucleotide, MMLV reverse transcriptase produced a good yield with 50 mM CsCl, mediocre yields with LiCl or without added alkali chloride, and a poor yield with 50 mM KCl. The full RT-PCR assay starting from the G4-prone polyribonucleotide, showed good results with CsCl in both stages, poor results with LiCl, and no product formation with KCl. The model polynucleotides showed fast G quadruplex formation under PCR or RT conditions with 50 mM KCl, but not with CsCl or LiCl. The results argue for the use of CsCl instead of KCl for RT and PCR of G4-prone sequences. No advantage was observed when using the 7-deaza type nucleotide analog c 7 dGTP in PCR amplification of the G4-prone polydeoxyribonucleotide. Copyright © 2017 Elsevier Inc. All rights reserved.
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Molecular analysis of dolphin morbillivirus: A new sensitive detection method based on nested RT-PCR.
Science.gov (United States)
Centelleghe, Cinzia; Beffagna, Giorgia; Zanetti, Rossella; Zappulli, Valentina; Di Guardo, Giovanni; Mazzariol, Sandro
2016-09-01
Cetacean Morbillivirus (CeMV) has been identified as the most pathogenic virus for cetaceans. Over the past three decades, this RNA virus has caused several outbreaks of lethal disease in odontocetes and mysticetes worldwide. Isolation and identification of CeMV RNA is very challenging in whales because of the poor preservation status frequently shown by tissues from stranded animals. Nested reverse transcription polymerase chain reaction (nested RT-PCR) is used instead of conventional RT-PCR when it is necessary to increase the sensitivity and the specificity of the reaction. This study describes a new nested RT-PCR technique useful to amplify small amounts of the cDNA copy of Cetacean morbillivirus (CeMV) when it is present in scant quantity in whales' biological specimens. This technique was used to analyze different tissues (lung, brain, spleen and other lymphoid tissues) from one under human care seal and seven cetaceans stranded along the Italian coastline between October 2011 and September 2015. A well-characterized, 200 base pair (bp) fragment of the dolphin Morbillivirus (DMV) haemagglutinin (H) gene, obtained by nested RT-PCR, was sequenced and used to confirm DMV positivity in all the eight marine mammals under study. In conclusion, this nested RT-PCR protocol can represent a sensitive detection method to identify CeMV-positive, poorly preserved tissue samples. Furthermore, this is also a rather inexpensive molecular technique, relatively easy to apply. Copyright © 2016 Elsevier B.V. All rights reserved.
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Identification of genes for normalization of real-time RT-PCR data in breast carcinomas
DEFF Research Database (Denmark)
Lyng, Maria B; Laenkholm, Anne-Vibeke; Pallisgaard, Niels
2008-01-01
BACKGROUND: Quantitative real-time RT-PCR (RT-qPCR) has become a valuable molecular technique in basic and translational biomedical research, and is emerging as an equally valuable clinical tool. Correlation of inter-sample values requires data normalization, which can be accomplished by various...... means, the most common of which is normalization to internal, stably expressed, reference genes. Recently, such traditionally utilized reference genes as GAPDH and B2M have been found to be regulated in various circumstances in different tissues, emphasizing the need to identify genes independent...... of factors influencing the tissue, and that are stably expressed within the experimental milieu. In this study, we identified genes for normalization of RT-qPCR data for invasive breast cancer (IBC), with special emphasis on estrogen receptor positive (ER+) IBC, but also examined their applicability to ER...
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Enseñanza aprendizaje del diseño arquitectónico y la educación para el desarrollo sostenible en universidades de Huancayo
Directory of Open Access Journals (Sweden)
Adolfo Concha Flores
2015-06-01
Full Text Available El objetivo fue describir las variables, enseñanza aprendizaje del diseño arquitectónico y la Educación para el Desarrollo Sostenible en las universidades de Huancayo, luego establecer sus correlaciones. Este trabajo de investigación fue de nivel descriptivo – correlacional, diseño no experimental, transversal. El instrumento de investigación fue la ficha de análisis de contenido, que para su validez se aplicó el Índice Kappa de Fleiss, que determinó un valor para ambas variables de 0,93 (acuerdo casi perfecto. La prueba de confiabilidad utilizó el Coeficiente Alfa de Cronbach cuya medida para ambas variables fue de 0,81 (Muy alto. La muestra utilizada fue no probabilístico, a juicio del investigador. Entre los resultados se pueden mencionar: la variable Enseñanza aprendizaje del diseño arquitectónico y la variable Fundamentación epistemológica de la EDS tienen puntuaciones de distribución de las medianas, con valores de Me=0,155 para la UNCP, Me=0,14 para la UPLA y Me=0,1375 para la UC, describiendo que existe acuerdos bajos entre estas. También se concretizaron un intervalo de desviación estándar con valores de 0,27 a -0,035 que determinaron una alta separación y la tendencia a bajos acuerdos entre las variables. Entre las conclusiones tenemos que existen acuerdos bajos entre las variables y de conformidad al coeficiente de correlación, la enseñanza de la arquitectura mantiene correlaciones mínimas, que permitió mostrar que se encuentra al margen del valor y la significancia que posee la Educación para el Desarrollo Sostenible.
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Comportamiento metabolico en el periparto de vacas Hartón del Valle, bajo condiciones de trópico bajo Metabolic behaviour in the peripartum period of dairy cows Hartón del Valle creole breed, under tropical conditions
Directory of Open Access Journals (Sweden)
Erika Andrea Hernández
2011-01-01
Full Text Available El periodo de periparto o periodo de transición presenta variaciones fisiológicas significativas que inciden en posteriores sucesos productivos (pico de lactancia, reactivación ovárica). El ganado Hartón posee una excelente eficiencia reproductiva (estrecho intervalo entre partos), factor que podría originarse en procesos metabólicos relacionados con un mejor ajuste homeostático. El principal objetivo del presente trabajo fue evaluar la homeostasis en una raza bovina criolla durante el periodo postparto. Para el estudio se emplearon 10 vacas multíparas, muestreadas antes del parto (días 30 y 15 preparto) y diariamente en los tres primeros días del parto; posteriormente a partir del día 5, con intervalos de cinco días y hasta el día 60 postparto. En total para cada animal se analizaron 14 muestras, cada una de ellas correspondió a un periodo analítico. Mediante venipunción coccígea en tubos al vacío con y sin anticoagulante se colectó la muestra de sangre completa. Por centrifugación se obtuvo plasma y suero, los cuales fueron almacenados a -20°C, hasta su análisis. Las determinaciones de hormonas se realizaron mediante radioinmunoanálisis (RIA) de fase sólida. A través de pruebas enzimáticas colorimétricas en equipos automatizados, se determinaron los valores de los metabolitos para cada uno de los periodos definidos. Los valores medios fueron: BHB (β-Hidroxibutirato) 0.39 mmol/L, NEFA (ácidos Grasos No Esterificados) 0.76 mmol/L, triglicéridos 0.42 mmol/L, colesterol 2.43 mmol/L, insulina 4.77 mµl/ml, triyodotironina (T3) 2.69 nmol/L, tetrayodotironina (T4) 57.37 nmol/L, progesterona 5.54 nmol/L, cortisol 32.42 nmol/L. Los valores obtenidos para los diferentes metabolitos se encuentran dentro de los rangos reportados por la literatura para bovinos en condiciones tropicales.The peripartum period or transition period, presents significant physiological changes that affect production in subsequent production events
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Simulating Rayleigh-Taylor (RT) instability using PPM hydrodynamics @scale on Roadrunner (u)
Energy Technology Data Exchange (ETDEWEB)
Woodward, Paul R [Los Alamos National Laboratory; Dimonte, Guy [Los Alamos National Laboratory; Rockefeller, Gabriel M [Los Alamos National Laboratory; Fryer, Christopher L [Los Alamos National Laboratory; Dimonte, Guy [Los Alamos National Laboratory; Dai, W [Los Alamos National Laboratory; Kares, R. J. [Los Alamos National Laboratory
2011-01-05
The effect of initial conditions on the self-similar growth of the RT instability is investigated using a hydrodynamics code based on the piecewise-parabolic-method (PPM). The PPM code was converted to the hybrid architecture of Roadrunner in order to perform the simulations at extremely high speed and spatial resolution. This paper describes the code conversion to the Cell processor, the scaling studies to 12 CU's on Roadrunner and results on the dependence of the RT growth rate on initial conditions. The relevance of the Roadrunner implementation of this PPM code to other existing and anticipated computer architectures is also discussed.
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Clinical utility of RT-PCR in assessing HER 2 gene expression versus traditional IHC and FISH in breast cancer patients.
Science.gov (United States)
Suryavanshi, Moushumi; Mehta, Anurag; Jaipuria, Jiten; Kumar, Dushyant; Vishwakarma, Gayatri; Panigrahi, Manoj Kumar; Verma, Haristuti; Saifi, Mumtaz; Sharma, Sanjeev; Tandon, Simran; Doval, D C; Das, Bhudev C
2018-02-09
IHC and FISH are used for categorizing HER 2 status in breast cancer at the protein and DNA level, respectively. HER 2 expression at the RNA level is quantitative, cheaper, easier to standardize and free from interobserver variation. 115 consecutive patients were tested by IHC, FISH and RT-PCR (test cohort). Assuming FISH result to be the response variable, ROC curves for RT-PCR ratio were analyzed to label HER 2 negative, equivocal and positive cases as RT-PCR score 1, 2 and 3, respectively. Inter-relationships between RT-PCR, IHC and FISH were defined. 'Clinical benefit' of a test was defined as proportion of patients labeled unequivocally as HER 2 positive or negative. Population for 1 year was simulated constraint to previous reports of HER 2 positivity and IHC category distribution by a meta-analysis of previous studies that evaluated concordance between IHC and FISH to determine HER 2 status (simulation cohort). Four diagnostic pathways in the simulation cohort were defined-(1) initial IHC, followed by FISH (conventional pathway); (2) initial RT-PCR, followed by FISH; (3) initial IHC, followed by RT-PCR and then by FISH; (4) initial RT-PCR, followed by IHC and then by FISH. The clinical benefit of IHC and RT-PCR in the four pathways was analyzed and sensitivity analysis for incremental cost-effectiveness ratio and cost-benefit comapring RT-PCR against IHC, both as first-line tests and among those with IHC score 2 as a reflex second-line test was performed by the Monte Carlo technique. 115 patients comprised the study population. While none with IHC score of 0 or 1 was FISH positive for HER 2, all cases with IHC score of 3 were FISH positive. 43 cases were assigned IHC score of 2. Thus, 72 patients benefited from the initial IHC testing [clinical benefit 62.6%], with the overall concordance between IHC and FISH being 100% for those with IHC score of 0, 1 and 3 (conclusive IHC categories). For RT-PCR with 100% concordance, 15.7% (115-97 = 18) patients
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E-government strategy of Surabaya city government through e-rt / rw to improve the quality of public service
Science.gov (United States)
Oktariyanda, T. A.; Rahaju, T.
2018-01-01
The use of information technology by Surabaya City Government has generated various innovations of public services such as “e-Sapa Warga”, e-Commerce, “e-RT/RW”, e-Budgeting, e-Project, e-Procurement, “e-Pendidikan”. Among the many innovations, e-RT/RW (Rukun Tetangga / Rukun Warga) is an innovation that is quite prominent because there are still many areas that have not implemented. This research can be classified as literature review and critical research. The aims of this research are to describe and analyze e-government strategy in Surabaya City through e-RT / RW to improve public service quality. The result of this research is e-government strategy in Surabaya City through e-RT / RW is already relevant and in accordance with Goldsmith’s theory of e-government. The positive impact of the implementation of e-RT / RW is the increasing quality of public services to bring benefits to all citizens Surabaya City.
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Prevalencia de consumo riesgoso y dañino de alcohol en derechohabientes del Instituto Mexicano del Seguro Social The prevalence of hazardous and harmful use of alcohol among the insured population of Instituto Mexicano del Seguro Social
Directory of Open Access Journals (Sweden)
Juana Inés de la Cruz Morales-García
2002-04-01
Full Text Available Objetivo. Medir la prevalencia de consumo riesgoso y dañino de alcohol en la población amparada por el Instituto Mexicano del Seguro Social (IMSS. Material y métodos. En un estudio transversal se entrevistaron 45 117 derechohabientes del IMSS, en las 36 delegaciones del país. Se aplicó un cuestionario estructurado y autoaplicable a cada sujeto usando el instrumento de tamizaje AUDIT (Alcohol Use Disorder Identification Test, el cual consta de 10 preguntas. Las tres primeras se relacionan con la cantidad y frecuencia del consumo de alcohol, las tres siguientes evalúan la dependencia y las cuatro últimas se refieren a problemas causados por el alcohol. Cada pregunta consta de tres a cinco opciones, a las que se les asignan valores progresivos de cero a cuatro. Se estimó la prevalencia de consumo, con intervalos de confianza al 95% (IC 95%. Resultados. La prevalencia de consumo problema de alcohol (riesgoso y dañino fue de 12.8% (IC 95% 12.5-13.2. El consumo fue mayor en hombres (22.2%; IC 95% 21.7-22.8 que en mujeres (3.4%; IC 95% 3.1-3.6. En los hombres se observó un efecto de edad, mientras que en las mujeres el consumo por edad fue más homogéneo. En ambos grupos el consumo fue más importante en la edad productiva. Conclusiones. La prevalencia de consumo de alcohol que constituye un problema para la salud, es elevada en la población derechohabiente del IMSS. Es necesario desarrollar políticas y programas de salud específicos, para disminuir este grave problema.Objective. To estimate the prevalence of hazardous and harmful alcohol consumption among the insured population of Instituto Mexicano del Seguro Social (Mexican Social Security Institute, IMSS. Material and Methods. A cross-sectional survey was conducted in 45,117 insured subjects from Mexico's 36 political districts; study subjects were interviewed using a structured and self-applied questionnaire on alcohol consumption, using the ten-item screening instrument AUDIT
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Challenges in Transitioning Research Data to Operations: The SPoRT Paradigm
Science.gov (United States)
Jedloved, Gary J.; Smith, Matt; McGrath, Kevin
2010-01-01
Established in 2002 to demonstrate the weather and forecasting application of real-time EOS measurements, the NASA Short-term Prediction Research and Transition (SPoRT) program has grown to be an end-to-end research to operations activity focused on the use of advanced NASA modeling and data assimilation approaches, nowcasting techniques, and unique high-resolution multispectral data from EOS satellites to improve short-term weather forecasts on a regional and local scale. With the ever-broadening application of real-time high resolution satellite data from current EOS and planned NPP, JPSS, and GOES-R sensors to weather forecast problems, significant challenges arise in the acquisition, delivery, and integration of the new capabilities into the decision making process of the operational weather community. For polar orbiting sensors such as MODIS, AIRS, VIIRS, and CRiS, the use of direct broadcast ground stations is key to the real-time delivery of the data and derived products in a timely fashion. With the ABI on the geostationary GOES-R satellite, the data volume will likely increase by a factor of 5- 10 from current data streams. However, the high data volume and limited bandwidth of end user facilities presents a formidable obstacle to timely access to the data. This challenge can be addressed through the use of subsetting techniques, innovative web services, and the judicious selection of data formats. Many of these approaches have been implemented by SPoRT for the delivery of real-time products to NWS forecast offices and other weather entities. Once available in decision support systems like AWIPS II, these new data and products must be integrated into existing and new displays that allow for the integration of the data with existing operational products in these systems. SPoRT is leading the way in demonstrating this enhanced capability. This paper will highlight the ways SPoRT is overcoming many of the challenges presented by the enormous data volumes of
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Enhancing the sensitivity of Dengue virus serotype detection by RT-PCR among infected children in India.
Science.gov (United States)
Ahamed, Syed Fazil; Vivek, Rosario; Kotabagi, Shalini; Nayak, Kaustuv; Chandele, Anmol; Kaja, Murali-Krishna; Shet, Anita
2017-06-01
Dengue surveillance relies on reverse transcription-polymerase chain reaction (RT-PCR), for confirmation of dengue virus (DENV) serotypes. We compared efficacies of published and modified primer sets targeting envelope (Env) and capsid-premembrane (C-prM) genes for detection of circulating DENV serotypes in southern India. Acute samples from children with clinically-diagnosed dengue were used for RT-PCR testing. All samples were also subjected to dengue serology (NS1 antigen and anti-dengue-IgM/IgG rapid immunochromatographic assay). Nested RT-PCR was performed on viral RNA using three methods targeting 654bp C-prM, 511bp C-prM and 641bp Env regions, respectively. RT-PCR-positive samples were validated by population sequencing. Among 171 children with suspected dengue, 121 were dengue serology-positive and 50 were dengue serology-negative. Among 121 serology-positives, RT-PCR detected 91 (75.2%) by CprM654, 72 (59.5%) by CprM511, and 74 (61.1%) by Env641. Among 50 serology-negatives, 10 (20.0%) were detected by CprM654, 12 (24.0%) by CprM511, and 11 (22.0%) by Env641. Overall detection rate using three methods sequentially was 82.6% (100/121) among serology-positive and 40.0% (20/50) among serology-negative samples; 6.6% (8/120) had co-infection with multiple DENV serotypes. We conclude that detection of acute dengue was enhanced by a modified RT-PCR method targeting the 654bp C-prM region, and further improved by using all three methods sequentially. Copyright © 2017 Elsevier B.V. All rights reserved.
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Detection of canine distemper virus nucleoprotein gene by RT-PCR in urine of dogs with distemper clinical signs
International Nuclear Information System (INIS)
Gebara, C.M.S.; Wosiachi, S.R.; Negrao, F.J.; Oliveira, D.B. de; Beloni, S.N.E.; Alfieri, A.A.; Alfieri, A.F.
2004-01-01
The urine of 87 dogs with clinical signs suggestive of canine distemper was analyzed by RT-PCR for detection of canine distemper virus (CDV) nucleoprotein gene. The samples were allotted to the following groups: group A- with 41 dogs with systemic symptoms, group B- with 37 dogs with neurological signs, and group C- with 9 dogs with simultaneous systemic and neurological clinical signs. Group D (control) included 20 assymptomatic dogs. A c2 was used to test RT-PCR results according to clinical form and hematological characteristics. The RT-PCR was positive for CDV in 47% (41/87) of the urine samples from dogs with clinical signs. All samples from assymptomatic dogs were RT-PCR negatives. Positive samples were found in all groups of dogs with distemper symptoms according to the following propositions: 51.2% (21/41), 29% (11/37) and 100% (9/9) for groups A, B and C, respectively. In all clinical forms (groups A, B and C) leucocytosis was the most frequent observed hematological alteration. No relationship between RT-PCR results and hematological changes was observed. The results showed that independently of the clinical stage of the illness the RT-PCR based on urine sample can be applied for ante mortem diagnosis of CDV
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Evaluación de la gestión de los casos de incapacidad temporal por contingencia común de más de 15 días en Cataluña Evaluation of the management of nonwork-related sick leave lasting more than 15 days in Catalonia (Spain
Directory of Open Access Journals (Sweden)
Fernando G. Benavides
2010-06-01
Full Text Available Objetivos: Comparar la duración de los casos de incapacidad temporal por contingencia común gestionados por las mutuas con los gestionados por el Instituto Nacional de la Seguridad Social (INSS. Métodos: Cohorte retrospectiva de 289.686 casos de incapacidad de más de 15 días de duración iniciados en 2005 tras su certificación por un médico de primaria en Cataluña (156.676 gestionados por el INSS, registrados en el Institut Català d'Avaluacions Mèdiques, y seguidos hasta su alta. La duración mediana y los percentiles fueron calculados con el estimador de Wang-Chang, que tiene en cuenta los episodios repetidos (25% aproximadamente, y se compararon mediante un modelo de regresión log-logístico con fragilidad gamma compartida, lo que permite estimar la razón de tiempo (RT y su intervalo de confianza del 95% (IC95%. Resultados: La duración mediana fue de 43 días para los casos del INSS y de 39 para los de mutuas. Esta diferencia fue estadísticamente significativa para los hombres del régimen general (RT=0,87; IC95%: 0,85-0,88 y del régimen de autónomos (RT=0,78; IC95%: 0,75-0,80, y para las mujeres del régimen general (RT=0,85; IC95%: 0,84-0,87 y de autónomos (RT=0,84; IC95%: 0,81-0,88; diferencias que se mantienen en general después de ajustar por la edad y la región sanitaria. Conclusiones: Los resultados confirman una finalización de los episodios más rápida para los casos de más de 15 días de duración gestionados por una mutua que para los gestionados por el INSS, tanto aquellos del régimen general como de autónomos. Es necesario investigar las causas de estas diferencias.Objectives: To compare the length of nonwork-related sick leave among cases managed by an insurance company versus those managed by the National Institute of Social Security (NISS. Methods: We performed a retrospective cohort study of 289,686 cases of sick leave lasting for more than 15 days that began in 2005 after certification by a primary
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Efeitos de esmolol, lidocaína e fentanil nos intervalos dispersão da onda P, QT, QTc e respostas hemodinâmicas à intubação endotraqueal durante indução com propofol: um estudo comparativo
Directory of Open Access Journals (Sweden)
Volkan Hancı
2013-06-01
Full Text Available JUSTIFICATIVA E OBJETIVOS: Investigar o efeito de esmolol, lidocaína e fentanil na dispersão da onda P (DP, durações dos intervalos QT e QT corrigido (QTc e as respostas hemodinâmicas à intubação endotraqueal durante a indução com propofol. MÉTODOS: Foram incluídos 80 pacientes adultos, estado físico ASA I ou II, idade entre 18 e 60 anos, neste estudo prospectivo, randômico e duplo-cego. Todos os pacientes foram submetidos a exame eletrocardiográfico (ECG antes da indução da anestesia. Os pacientes foram randomicamente alocados em quatro grupos iguais. O grupo controle (Grupo C recebeu 5 mL de solução salina; o grupo esmolol (Grupo E recebeu 0,5 mg.kg-1 de esmolol; o grupo fentanil (Grupo F recebeu 2 µg.kg-1 de fentanil e o grupo lidocaína (Grupo L recebeu 1,5 mg.kg-1 de lidocaína antes da indução anestésica. A anestesia foi induzida com propofol. ECG foi feito em todos os pacientes durante o primeiro e o terceiro minutos de indução, 3 minutos após a administração de relaxante muscular e 5 e 10 minutos após intubação. A DP e intervalos QT foram medidos em todos os ECGs. Os intervalos QTc foram determinados com o uso da fórmula de Bazett. Frequência cardíaca (FC e pressão arterial média (PAM foram registradas antes e depois da indução anestésica, imediatamente após a intubação e em 1, 3, 5, 7 e 10 minutos após a intubação. RESULTADOS: Após a intubação, a FC aumentou significativamente nos Grupos C, L e F em comparação com o grupo controle. Porém, não houve diferença significativa nos valores da FC após a intubação entre os grupos E e controle. Nos Grupos C e L, a PAM aumentou significativamente após a intubação em comparação com o grupo controle. No entanto, nos Grupos L, F e E não houve diferença significativa entre os valores da PAM após a intubação em comparação com o grupo controle. A DP foi significativamente mais longa no Grupo C após a intubação em comparação com o
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From my Home: Dvarionas: Elegie; Pärt: Fratres / Patric Wiklacz
Index Scriptorium Estoniae
Wiklacz, Patric
1997-01-01
Uuest heliplaadist "From my Home: Dvarionas: Elegie; Pärt: Fratres; Barkauskas: Partita; Vasks: Musica Dolorosa; Pelecis: Concerto for Violin, Piano and String Orchestra, "Neverthless"; Plakidis: Two Grasshopper Dances; Tüür: Conversio. Deutsche Kammerphilharmonie, Gidon Kremer (violon), Vadim Sacharov (piano)" Teldec 0630-14654-2 (79 minutes:DDD)
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Rendimiento y calidad de forraje del pasto ovillo (Dactylis glomerata L. al variar la frecuencia e intensidad de pastoreo
Directory of Open Access Journals (Sweden)
Jorge Armando Villareal González
2014-01-01
Full Text Available El objetivo fue determinar el rendimiento y calidad del pasto ovillo ( Dactylis glomerata L., al variar la frecuencia e intensidad de pastoreo. Se evaluaron tres frecuencias (2, 3 y 4 semanas en primavera y verano y 4, 5 y 6 semanas durante otoño y dos intensidades de pastoreo (severa: 3 a 5 cm y ligera: 6 a 8 cm de altura de forr aje residual. Se evaluó el rendimiento de forraje, tasa de acumulación neta de forraje (TANF, composición morfoló gica y calidad del forraje. El mayor rendimiento acumulado y TANF se presentaron con pastoreo severo cada 4 y 6 sem anas y en ambas intensidades cada 3 y 5 semanas. El mayor rendimiento estacional (7,844; 7,699; 7114 kg MS ha -1 se presentó en verano en las mismas frecuencias e intensidades de pastoreo, respectivamente ( P <0.05. Durante primavera no se observaron dife rencias en rendimiento y TANF entre frecuencias de pastoreo. En otoño la frecuencia de 5 semanas superó a la de 4 ( P <0.05, pero no diferente a la de 6 semanas. En todas las estaciones del año la intensidad de pastoreo severo superó a la ligera, siendo diferentes en primavera y verano ( P <0.05. En general, el contenido de proteína total y digestibilidad in vitro de la materia seca tendieron a disminuir conforme aumentó el intervalo de pastoreo, siendo en promedio de 20 y 65 %, respectivamente. Se concluye que para obtener el mayor re ndimiento y calidad de forraje, los pastoreos deben realizarse cada 4 semanas durante primavera-verano y cada 5 semanas en otoño, a una intensidad severa.
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La escoba de bruja del cacao [Crinipellis perniciosa (Stahel Singer] en la región del Piedemonte Llanero de Colombia: la dinámica de la producción de escobas y el periodo de latencia
Directory of Open Access Journals (Sweden)
Tovar Germán
1991-06-01
Full Text Available El objetivo de esta investigación fue estudiar la dinámica de la formación de escobas en el tiempo, el periodo verde y el periodo seco de la escoba y Ia capacidad de regeneración de las mismas después de su remoción. EI estudio se realizo durante varios años en tres plantaciones hibridas de cacao de 5, 7 Y 12 años. Las tasas de infección variaron de un año a otro y estuvieron relacionadas con la cantidad de inóculo inicial (Xo. La proporción de enfermedad de un año a otro o incremento anual del numero de escobas fue relativamente constante con un intervalo de variación entre 1: 1,5, Y 1: 2,5. La mayor parte de las escobas tuvieron un periodo verde entre 4 y 6 semanas. El periodo seco o de inducción de la fructificación del hongo fue variable y su duración depende de la época del año en que ocurre la infección, con un mínima de 14 semanas y un máximo de 54 semanas. EI ciclo de la enfermedad es predominantemente anual a monocíclico dentro de un año y poliético a través de varios años, lo cual determina la periocidad anual de la pacta fitosanitaria de las escobas para el control de Ia enfermedad. La brotación enferma después de la remoción de las escobas fue de 6,3% y su desarrollo fue escaso (3 em, lo cual sugiere que la poda fitosanitaria de las escobas es una medida eficaz desde el punto de vista de reducción del inóculo (xo, estrategia de control especialmente relevante.
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Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
Directory of Open Access Journals (Sweden)
Bruynseels Peggy
2009-07-01
Full Text Available Abstract We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample.
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Experimentation of RT-LOTOS for a critical function of a power plant instrumentation and control system; Experimentation de RT-LOTOS pour une fonction critique d`un systeme de controle-commande en centrale
Energy Technology Data Exchange (ETDEWEB)
Andriantsiiferana, L.; Courtiat, J.P.; De Oliveira, R.C.
1996-01-01
This document is the final report of the contract between LAAS-CNRS and the EdF Direction of Researches and Studies about the experimentation of RT-LOTOS formal description technique on a critical function of a nuclear power plant instrumentation and control system. This report comprises 4 parts: part 1 describes the RT-LOTOS (Real Time-Language of Temporal Ordering Specifications) tutorial and its RTL tool environment, the different RT-LOTOS operators and semantics, and the RTL tool main functionalities; part 2 deals with the methodology and the global architecture for the specification and validation of the CCT (Central Calculator for Treatment); part 3 gives more details about the different processes identified in the specification architecture and presents the main results obtained for the validation of the nominal functioning; part 4 describes some exceptional situations and shows how some failures and their associated recovery mechanisms can be modelled. (J.S.). 6 refs., 53 figs., 5 tabs., 2 appends.
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Request for regular monitoring of the symbiotic variable RT Cru
Science.gov (United States)
Waagen, Elizabeth O.
2014-08-01
Dr. Margarita Karovska (Harvard-Smithsonian Center for Astrophysics) and colleagues have requested AAVSO observer assistance in their campaign on the symbiotic variable RT Cru (member of a new class of hard X-ray emitting symbiotic binaries). Weekly or more frequent monitoring (B, V, and visual) beginning now is requested in support of upcoming Chandra observations still to be scheduled. "We plan Chandra observations of RT Cru in the near future that will help us understand the characteristics of the accretion onto the white dwarf in this sub-class of symbiotics. This is an important step for determining the precursor conditions for formation of a fraction of asymmetric Planetary Nebulae, and the potential of symbiotic systems as progenitors of at least a fraction of Type Ia supernovae." Finder charts with sequence may be created using the AAVSO Variable Star Plotter (http://www.aavso.org/vsp). Observations should be submitted to the AAVSO International Database. See full Alert Notice for more details and observations.
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Comportamiento clínico del síndrome dolor disfunción del aparato temporomandibular en una consulta de urgencias estomatológicas Clinical behavior of the dysfunction pain temporomandibular joint syndrome assessed in a Stomatology emergence consultation
Directory of Open Access Journals (Sweden)
Yudit Algozaín Acosta
2009-06-01
Full Text Available Introducción: el síndrome dolor disfunción del aparato temporomandibular (SDDAT comprende el conjunto de signos y síntomas como resultado de las alteraciones cuantitativas y cualitativas de la función de los componentes del aparato masticatorio. Se presenta con frecuencia y resulta molesto para el paciente, por lo que se realiza este estudio, con el objetivo de caracterizarlo clínicamente. Métodos: se realizó un estudio prospectivo, descriptivo, de corte transversal, con los pacientes que acudieron al Servicio de Urgencias de la Clínica Estomatológica de Artemisa, en el período comprendido entre julio de 2007 hasta marzo de 2008 con el diagnóstico de SDDAT. Resultados: del total de pacientes atendidos solo el 1,1 % presentó un diagnóstico de SDDAT. El intervalo de edad de mayor frecuencia fue el de 22 a 59 años, el dolor a la masticación y el ruido articular fueron el síntoma y el signo predominante, respectivamente. Se identificaron como factores de riesgo principales el estrés y el bruxismo. Más del 67 % de los pacientes eran del sexo femenino, en las cuales apareció la mayor recurrencia del síndrome. Conclusiones: la población del municipio de Artemisa presentó una baja incidencia del SDDAT en la consulta de urgencias estomatológicas, encontrándose una asociación estadísticamente significativa entre el sexo femenino y la aparición de este síndrome, donde el estrés desempeña un papel importante.Introduction: Dysfunction pain temporomandibular joint syndrome (DPTJS includes signs and symptoms as a result of quantitative and qualitative alterations of the masticatory tract component function. Is frequently present and annoying for patient, thus we made this study to clinically characterize it. Methods: We made a cross-sectional, descriptive and prospective study of patients seen in Emergence Service of Stomatology Clinic in Artemisa municipality from July, 2007 and March, 2008 diagnosing DPTJS. Results: From the
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Efeitos do intervalo de recuperação entre as séries sobre a pressão arterial após exercícios resistidos Efectos del intervalo de recuperación entre las series sobre la presión arterial luego de ejercicios de resistencia Effects of rest interval between exercise sets on blood pressure after resistance exercises
Directory of Open Access Journals (Sweden)
João Veloso
2010-04-01
Full Text Available FUNDAMENTO: Apesar de os estudos demonstrarem a ocorrência da hipotensão pós-exercício (HPE em exercícios resistidos, ainda não há consenso sobre um protocolo ideal. OBJETIVO: Avaliar os efeitos de diferentes intervalos de recuperação (IR entre séries de exercício resistido (ER na pressão arterial (PA pós-exercício. MÉTODOS: Dezesseis homens jovens sedentários e não hipertensos realizaram, de forma contrabalanceada três protocolos de ER com 1 (P1, 2 (P2 e 3 (P3 minutos de IR entre as séries, e um protocolo controle (CON. Os protocolos de ER consistiram em três séries de oito repetições em seis exercícios. As cargas utilizadas na 1ª, 2ª e 3ª série dos exercícios foram respectivamente 80, 70 e 60% de 1 repetição máxima (1RM. As medidas foram realizadas em repouso (REP, 15 (T15, 30 (T30, 45 (T45, 60 (T60, 75 (T75 e 90 (T90 minutos após a sessão. Foi realizada uma análise de variância fatorial (ANOVA com post hoc LSD. RESULTADOS: Não foi encontrada alteração significativa na PA sistólica após os protocolos. Foi encontrado um aumento significativo da PA diastólica, após o CON, nos momentos T45 e T90. Ocorreu redução significativa na PA diastólica após P1 e P3, com duração de 30 e 15 minutos respectivamente. Não foram encontradas diferenças significativas nas respostas da PA sistólica e diastólica entre os protocolos com diferentes IR. CONCLUSÃO: Parece que o IR não influencia a redução da PA sistólica após uma sessão de ER. No entanto, foi encontrada uma queda na PA diastólica (P1 e P3 com duração de até 30 minutos.FUNDAMENTO: A pesar de que los estudios demuestran la aparición de hipotensión post-ejercicio (HPE en Ejercicios de Resistencia, aún no existe consenso sobre un protocolo ideal. OBJETIVO: Evaluar los efectos de diferentes intervalos de recuperación (IR entre series de ejercicios de resistencia (ER en la presión arterial (PA post-ejercicio. MÉTODOS: Dieciséis hombres j
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Modeling variably saturated multispecies reactive groundwater solute transport with MODFLOW-UZF and RT3D
Science.gov (United States)
Bailey, Ryan T.; Morway, Eric D.; Niswonger, Richard G.; Gates, Timothy K.
2013-01-01
A numerical model was developed that is capable of simulating multispecies reactive solute transport in variably saturated porous media. This model consists of a modified version of the reactive transport model RT3D (Reactive Transport in 3 Dimensions) that is linked to the Unsaturated-Zone Flow (UZF1) package and MODFLOW. Referred to as UZF-RT3D, the model is tested against published analytical benchmarks as well as other published contaminant transport models, including HYDRUS-1D, VS2DT, and SUTRA, and the coupled flow and transport modeling system of CATHY and TRAN3D. Comparisons in one-dimensional, two-dimensional, and three-dimensional variably saturated systems are explored. While several test cases are included to verify the correct implementation of variably saturated transport in UZF-RT3D, other cases are included to demonstrate the usefulness of the code in terms of model run-time and handling the reaction kinetics of multiple interacting species in variably saturated subsurface systems. As UZF1 relies on a kinematic-wave approximation for unsaturated flow that neglects the diffusive terms in Richards equation, UZF-RT3D can be used for large-scale aquifer systems for which the UZF1 formulation is reasonable, that is, capillary-pressure gradients can be neglected and soil parameters can be treated as homogeneous. Decreased model run-time and the ability to include site-specific chemical species and chemical reactions make UZF-RT3D an attractive model for efficient simulation of multispecies reactive transport in variably saturated large-scale subsurface systems.
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Pärt: kultuurilis-diplomaatiline missioon Vatikanis täidetud / Valner Valme
Index Scriptorium Estoniae
Valme, Valner, 1970-
2011-01-01
23. augustil Katariina kirikus toimunud uue singli "Vater Unser" esitlusest. Selle pala kinkis A. Pärt paavstile 4. juulil Vatikanis. Esitlusel viibis ka president Toomas Hendrik Ilves. Pärdi päevade raames toimuvatest üritustest
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La divergencia genética entre poblaciones del Área Andina Centro Meridional evaluada mediante rasgos no métricos del cráneo
Directory of Open Access Journals (Sweden)
Cocilovo, José Alberto
2009-01-01
Full Text Available Durante más de 10.000 años el Area Andina Centro Meridional proporcionó un escenario ideal para el desarrollo de distintas poblaciones y entidades culturales, interactuando a través de una amplia red de intercambio y distribución de productos. A pesar de este nivel de interacción, la información métrica disponible (Bolivia, Norte de Chile y Noroeste Argentino, reveló un fuerte proceso de divergencia genética (FST = 0.195 entre subregiones (Varela et al., 2008. Esta evidencia es contrastada en el presente trabajo a partir del análisis de una muestra integrada por 1416 individuos de ambos sexos, cubriendo un intervalo de 4.500 años. Se emplearon 12 atributos (rasgos no métricos del cráneo registrados como presencia-ausencia. Las diferencias entre subáreas fueron evaluadas mediante la estadística MMDS y D2 de Mahalanobis calculada a partir de componentes principales. Ambas matrices de distancias fenotípicas presentaron una alta correlación, destacando una significativa diferenciación a nivel regional. La mayor distancia se registra entre el Noroeste Argentino y el Norte de Chile, ocupando Bolivia una posición equidistante entre ambas regiones. Dentro de cada región las muestras están más relacionadas entre si ((Cochabamba, (Puna, Quebrada, Valliserrana y Pampa Grande, (Arica, Pisagua, Norte Semiárido. Hay mayor vinculación entre Cochabamba y el Noroeste Argentino y mayor divergencia entre los grupos de Chile. Se confi rma un modelo de poblamiento a partir de la subdivisión de una población ancestral en dos ramas que ocuparon: una el Norte de Chile y otra el Noroeste Argentino. En cada una de ellas el proceso dispersivo originó varias líneas que se diferenciaron gradualmente hacia el sur, durante la exploración de nuevos ambientes cuya conquista y colonización garantizó la subsistencia de la población.
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La superaci??n del modelo del ??ngel del hogar:
OpenAIRE
Hurtado Mu??oz, M??nica
2012-01-01
La Tesis Doctoral titulada "La superaci??n del modelo del "??ngel del hogar". Recuperaci??n de la escritora Leonor Canalejas y Fustegueras (1869-1945)" aborda, en primer lugar, un an??lisis sociol??gico de las circunstancias que rodearon a la mujer espa??ola en el cambio de siglo en el ??mbito social y educativo, en un intento de esclarecer las caracter??sticas propias del contexto en el que la mujer accede a la intelectualidad en general y a la literatura en particular. Esta parte concluye q...
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iSmaRT: a toolkit for a comprehensive analysis of small RNA-Seq data.
Science.gov (United States)
Panero, Riccardo; Rinaldi, Antonio; Memoli, Domenico; Nassa, Giovanni; Ravo, Maria; Rizzo, Francesca; Tarallo, Roberta; Milanesi, Luciano; Weisz, Alessandro; Giurato, Giorgio
2017-03-15
The interest in investigating the biological roles of small non-coding RNAs (sncRNAs) is increasing, due to the pleiotropic effects of these molecules exert in many biological contexts. While several methods and tools are available to study microRNAs (miRNAs), only few focus on novel classes of sncRNAs, in particular PIWI-interacting RNAs (piRNAs). To overcome these limitations, we implemented iSmaRT ( i ntegrative Sm all R NA T ool-kit), an automated pipeline to analyze smallRNA-Seq data. iSmaRT is a collection of bioinformatics tools and own algorithms, interconnected through a Graphical User Interface (GUI). In addition to performing comprehensive analyses on miRNAs, it implements specific computational modules to analyze piRNAs, predicting novel ones and identifying their RNA targets. A smallRNA-Seq dataset generated from brain samples of Huntington's Disease patients was used here to illustrate iSmaRT performances, demonstrating how the pipeline can provide, in a rapid and user friendly way, a comprehensive analysis of different classes of sncRNAs. iSmaRT is freely available on the web at ftp://labmedmolge-1.unisa.it (User: iSmart - Password: password). aweisz@unisa.it or ggiurato@unisa.it. Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com
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Pärt, Arvo: De profundis. Missa Sillabica. Solfeggio / Marc Rochester
Index Scriptorium Estoniae
Rochester, Marc
1997-01-01
Uuest heliplaadist "Pärt, Arvo: De profundis. Missa Sillabica. Solfeggio. And one of the Pharisees. Cantate Domino. Summa. Seven Magnificat Antiphons. The Beautitudes. Magnificat. Christopher Bowers-Broadbent (org.), Daniel Kennedy (perc.). Theatre of Voices / Paul Hillier (bass). Harmonia Mundi HMU40 7182; HMU90 7182 (76 minutes:DDD)"
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Analysis of gene expression in small numbers of purified hemopoietic progenitor cells by RT-PCR.
Science.gov (United States)
Ziegler, B L; Lamping, C P; Thoma, S J; Fliedner, T M
1995-05-01
Primitive hemopoietic stem cells represent the most probable targets for genetic alterations due to exposure to ionizing irradiation or chemical carcinogens. We have applied a two-step protocol for the purification of CD34+HLA-DR-/low hemopoietic progenitor cells from cord blood (CB). CD34+ cells were isolated by monoclonal antibody (mAb) against CD34 (My10) and immunomagnetic beads. Beads were cleaved off the CD34+ cells by enzymatic treatment with chymopapain. Due to chymopapain-resistance of epitopes recognized by the used mAbs purity control of CD34+ cells and separation into CD34+HLA-DR-/low and CD34+HLA-DR+ subsets could be performed by using flow cytometry. Two miniaturized procedures were applied to isolate poly(A)+ mRNA for the reverse transcription polymerase chain reaction (RT-PCR) from small numbers of CD34+HLA-DR-/low cells. In five experiments, the mean purity of immunomagnetically isolated CD34+ cells was 93.8% +/- 3.9. Flow cytometry sorting of CD34+ cells resulted in pure CD34+HLA-DR-/low populations (purity > 98.8%; range 98.8% to 99.9%; viability > 96%) with an average yield of 2600 +/- 800 cells/5 x 10(7) low density CB cells. By RT-PCR using both poly(A)+ mRNA isolation procedures, sequences corresponding to CD34 and beta 2-microglobulin were amplified from as few as 20 cells. Furthermore, a sequence-independent RT-PCR (SIP-RT-PCR) was applied to amplify the cDNA derived from five erythroblasts isolated from a burst-forming unit-erythroid (BFU-E). Upon hybridization, full-length c-fos message was detected in the SIP-RT-PCR amplified material. Our data demonstrate that gene expression can be detected at the transcriptional level in small numbers of hemopoietic progenitor cells. In addition, the SIP-RT-PCR may allow the amplification of unique mRNA species when subtractive hybridization procedures are performed. The presented data should be useful to analyze gene expression in rare subsets of radiation-exposed immature hemopoietic stem
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Applications of LANCE Data at SPoRT
Science.gov (United States)
Molthan, Andrew
2014-01-01
Short term Prediction Research and Transition (SPoRT) Center: Mission: Apply NASA and NOAA measurement systems and unique Earth science research to improve the accuracy of short term weather prediction at the regional/local scale. Goals: Evaluate and assess the utility of NASA and NOAA Earth science data and products and unique research capabilities to address operational weather forecast problems; Provide an environment which enables the development and testing of new capabilities to improve short term weather forecasts on a regional scale; Help ensure successful transition of new capabilities to operational weather entities for the benefit of society
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SMAP Data Assimilation at NASA SPoRT
Science.gov (United States)
Blankenship, Clay B.; Case, Jonathan L.; Zavodsky, Bradley T.
2016-01-01
The NASA Short-Term Prediction Research and Transition (SPoRT) Center maintains a near-real- time run of the Noah Land Surface Model within the Land Information System (LIS) at 3-km resolution. Soil moisture products from this model are used by several NOAA/National Weather Service Weather Forecast Offices for flood and drought situational awareness. We have implemented assimilation of soil moisture retrievals from the Soil Moisture Ocean Salinity (SMOS) and Soil Moisture Active/ Passive (SMAP) satellites, and are now evaluating the SMAP assimilation. The SMAP-enhanced LIS product is planned for public release by October 2016.
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Cognition and Quality of Life After Chemotherapy Plus Radiotherapy (RT) vs. RT for Pure and Mixed Anaplastic Oligodendrogliomas: Radiation Therapy Oncology Group Trial 9402
International Nuclear Information System (INIS)
Wang Meihua; Cairncross, Gregory; Shaw, Edward
2010-01-01
Purpose: Radiation Therapy Oncology Group 9402 compared procarbazine, lomustine, and vincristine (PCV) chemotherapy plus radiation therapy (PCV + RT) vs. RT alone for anaplastic oligodendroglioma. Here we report longitudinal changes in cognition and quality of life, effects of patient factors and treatments on cognition, quality of life and survival, and prognostic implications of cognition and quality of life. Methods and Materials: Cognition was assessed by Mini Mental Status Examination (MMSE) and quality of life by Brain-Quality of Life (B-QOL). Scores were analyzed for survivors and within 5 years of death. Shared parameter models evaluated MMSE/B-QOL with survival. Results: For survivors, MMSE and B-QOL scores were similar longitudinally and between treatments. For those who died, MMSE scores remained stable initially, whereas B-QOL slowly declined; both declined rapidly in the last year of life and similarly between arms. In the aggregate, scores decreased over time (p = 0.0413 for MMSE; p = 0.0016 for B-QOL) and were superior with age <50 years (p < 0.001 for MMSE; p = 0.0554 for B-QOL) and Karnofsky Performance Score (KPS) 80-100 (p < 0.001). Younger age and higher KPS were associated with longer survival. After adjusting for patient factors and drop-out, survival was longer after PCV + RT (HR = 0.66, 95% CI = 0.49-0.9, p = 0.0084; HR = 0.74, 95% CI = 0.54-1.01, p = 0.0592) in models with MMSE and B-QOL. In addition, there were no differences in MMSE and B-QOL scores between arms (p = 0.4752 and p = 0.2767, respectively); higher scores predicted longer survival. Conclusion: MMSE and B-QOL scores held steady in the upper range in both arms for survivors. Younger, fitter patients had better MMSE and B-QOL and longer survival.
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Superior sulcus non-small cell lung carcinoma: A comparison of IMRT and 3D-RT dosimetry.
Science.gov (United States)
Truntzer, Pierre; Antoni, Delphine; Santelmo, Nicola; Schumacher, Catherine; Falcoz, Pierre-Emmanuel; Quoix, Elisabeth; Massard, Gilbert; Noël, Georges
2016-01-01
A dosimetric study comparing intensity modulated radiotherapy (IMRT) by TomoTherapy to conformational 3D radiotherapy (3D-RT) in patients with superior sulcus non-small cell lung cancer (NSCLC). IMRT became the main technique in modern radiotherapy. However it was not currently used for lung cancers. Because of the need to increase the dose to control lung cancers but because of the critical organs surrounding the tumors, the gains obtainable with IMRT is not still demonstrated. A dosimetric comparison of the planned target and organs at risk parameters between IMRT and 3D-RT in eight patients who received preoperative or curative intent irradiation. In the patients who received at least 66 Gy, the mean V95% was significantly better with IMRT than 3D-RT (p = 0.043). IMRT delivered a lower D2% compared to 3D-RT (p = 0.043). The IH was significantly better with IMRT (p = 0.043). The lung V 5 Gy and V 13 Gy were significantly higher in IMRT than 3D-RT (p = 0.043), while the maximal dose (D max) to the spinal cord was significantly lower in IMRT (p = 0.043). The brachial plexus D max was significantly lower in IMRT than 3D-RT (p = 0.048). For patients treated with 46 Gy, no significant differences were found. Our study showed that IMRT is relevant for SS-NSCLC. In patients treated with a curative dose, it led to a reduction of the exposure of critical organs, allowing a better dose distribution in the tumor. For the patients treated with a preoperative schedule, our results provide a basis for future controlled trials to improve the histological complete response by increasing the radiation dose.
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Elaboração e validação de intervalos de referência longitudinais de peso fetal com uma amostra da população brasileira Elaboration and validation of longitudinal reference intervals of fetal weight with a sample of the Brazilian population
Directory of Open Access Journals (Sweden)
Érica Luciana de Paula Furlan
2012-10-01
Full Text Available OBJETIVOS: Elaborar modelos de predição de peso fetal e de percentis longitudinais de peso fetal estimado (PFE com uma amostra da população brasileira. MÉTODOS: Estudo observacional prospectivo. Dois grupos de gestantes foram recrutados: Grupo EPF (estimativa de peso fetal: pacientes para elaboração (EPF-El e validação (EPF-Val de um modelo de predição de peso fetal; Grupo IRL (intervalos de referência longitudinais: gestantes para elaboração (IRL-El e validação (IRL-Val de intervalos de referência longitudinais de PFE. Regressão polinomial foi utilizada com os dados do subgrupo EPF-El para gerar o modelo de predição de peso fetal. O desempenho deste modelo foi comparado com os de outros disponíveis na literatura. Modelos lineares mistos foram usados para elaboração de intervalos longitudinais de PFE com os dados do subgrupo IRL-El. Os dados do subgrupo IRL-Val foram usados para validação destes intervalos. RESULTADOS: Quatrocentos e cinqüenta e oito pacientes compuseram o Grupo EPF (EPF-El: 367; EPF-Val: 91 e 315 o Grupo IRL (IRL-El: 265; IRL-Val: 50. A fórmula para cálculo do PFE foi: PFE=-8,277+2,146xDBPxCAxCF-2,449xCFxDBP². Os desempenhos de outras fórmulas para estimativa de peso fetal em nossa amostra foram significativamente piores do que os do modelo gerado neste estudo. Equações para predição de percentis condicionais de PFE foram derivadas das avaliações longitudinais do subgrupo IRL-El e validadas com os dados do subgrupo IRL-Val. CONCLUSÕES: descrevemos um método para adaptação de intervalos de referência longitudinais de PFE, sendo este obtido por meio de fórmulas geradas em uma amostra da população brasileira.PURPOSES: To elaborate models for the estimation of fetal weight and longitudinal reference intervals of estimated fetal weight (EFW using a sample of the Brazilian population. METHODS: Prospective observational study. Two groups of patients were evaluated: Group EFW (estimation of
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Det retlige sprog som virkelighedsskabende - graviditetsvært kontra surrogatmoder
DEFF Research Database (Denmark)
Pedersen, Frank Høgholm
2018-01-01
I dag er det undtagelsen, at kvinden, der bærer graviditeten og føder barnet i et surrogati-arrangement, er genetisk relateret til barnet. I artiklen foreslås som en supplerende betegnelse til surrogatmoder derfor betegnelsen: graviditetsvært. Valget af en retlig betegnelse til beskrivelse af fak...
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How feasible is remote 3D dosimetry for MR guided Radiation Therapy (MRgRT)?
International Nuclear Information System (INIS)
Mein, S; Miles, D; Juang, T; Fenoli, J; Oldham, M; Rankine, L; Cai, B; Curcuru, A; Mutic, S; Li, H; Adamovics, J
2017-01-01
To develop and apply a remote dosimetry protocol with PRESAGE® radiochromic plastic and optical-CT readout in the validation of MRI guided radiation therapy (MRgRT) treatments (MRIdian® by ViewRay®). Through multi-institutional collaboration we performed PRESAGE® dosimetry studies in 4ml cuvettes to investigate dose-response linearity, MR-compatibility, and energy-independence. An open calibration field and symmetrical 3-field plans were delivered to 10cm diameter PRESAGE® to examine percent depth dose and response uniformity under a magnetic field. Evidence of non-linear dose response led to a large volume PRESAGE® study where small corrections were developed for temporally- and spatially-dependent behaviors observed between irradiation and delayed readout. TG-119 plans were created in the MRIdian® TPS and then delivered to 14.5cm 2kg PRESAGE® dosimeters. Through the domestic investigation of an off-site MRgRT system, a refined 3D remote dosimetry protocol is presented capable of validation of advanced MRgRT radiation treatments. (paper)
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How feasible is remote 3D dosimetry for MR guided Radiation Therapy (MRgRT)?
Science.gov (United States)
Mein, S.; Rankine, L.; Miles, D.; Juang, T.; Cai, B.; Curcuru, A.; Mutic, S.; Fenoli, J.; Adamovics, J.; Li, H.; Oldham, M.
2017-05-01
To develop and apply a remote dosimetry protocol with PRESAGE® radiochromic plastic and optical-CT readout in the validation of MRI guided radiation therapy (MRgRT) treatments (MRIdian® by ViewRay®). Through multi-institutional collaboration we performed PRESAGE® dosimetry studies in 4ml cuvettes to investigate dose-response linearity, MR-compatibility, and energy-independence. An open calibration field and symmetrical 3-field plans were delivered to 10cm diameter PRESAGE® to examine percent depth dose and response uniformity under a magnetic field. Evidence of non-linear dose response led to a large volume PRESAGE® study where small corrections were developed for temporally- and spatially-dependent behaviors observed between irradiation and delayed readout. TG-119 plans were created in the MRIdian® TPS and then delivered to 14.5cm 2kg PRESAGE® dosimeters. Through the domestic investigation of an off-site MRgRT system, a refined 3D remote dosimetry protocol is presented capable of validation of advanced MRgRT radiation treatments.
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Real time RT-PCR assay for detection of different serotypes of FMDV in Egypt
Directory of Open Access Journals (Sweden)
Laila El-Shehawy
Full Text Available Aim: The present study indicated that rRT-PCR could be provided for the detection of FMDV in infected, contact and carrier cattle and also provide a rapid sensitive tool aiming to aid in rapid disease detection and control. Foot and Mouth disease virus serotypes O and A still existing in Egypt. In January 2012, sever outbreaks struck the animal population in most Egyptian 1 governorates. The causative virus was identified as FMDV SAT2. Material and Methods: Five samples of tongue epithelium (ET and five oesophageal-pharyngeal (OP fluid samples were collected from FMD suspected cattle in infected farm at El-Fayoum and 20 OP samples from in-contact cattle at the same farm in addition to 30 OP samples from apparently healthy cattle at three different localities in El-Fayoum governorate (12 from Fayoum; 9 from Sinoras and 9 from Edsa in order to detect carrier cattle. All of these samples were collected during November and December 2011 and January 2012. Results: All the ET and OP samples were inoculated on BHK cell culture and baby mice. The obtained results were identified using complement fixation test in addition to real-time reverse transcriptase polymerase chain reaction (rRT-PCR. In the infected farm at El-Fayoum FMDV type SAT2 was detected in cattle which are considered as the first introduction of this type while FMDV type O and SAT2 were detected in the in-contact cattle in the same farm. The sensitivity of rRT-PCR was cleared in the in-contact cattle as 13 out of 20 OP samples were positive to FMDV by rRT-PCR while 11 out of 20 OP samples were positive to FMDV by CFT. The OP samples collected from apparently healthy cattle from Fayoum, Sinoras and Edsa localities in Fayoum governorate demonstrate the circulation of the FMDV type A, O and the recent SAT2 in carrier cattle which threaten cattle population in Fayoum governorate. Also the sensitivity of real time RT-PCR over the CFT in detection of FMDV carrier cattle was clearly noticed in
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Selection of reference genes for quantitative real time RT-PCR during dimorphism in the zygomycete Mucor circinelloides.
Science.gov (United States)
Valle-Maldonado, Marco I; Jácome-Galarza, Irvin E; Gutiérrez-Corona, Félix; Ramírez-Díaz, Martha I; Campos-García, Jesús; Meza-Carmen, Víctor
2015-03-01
Mucor circinelloides is a dimorphic fungal model for studying several biological processes including cell differentiation (yeast-mold transitions) as well as biodiesel and carotene production. The recent release of the first draft sequence of the M. circinelloides genome, combined with the availability of analytical methods to determine patterns of gene expression, such as quantitative Reverse transcription-Polymerase chain reaction (qRT-PCR), and the development of molecular genetic tools for the manipulation of the fungus, may help identify M. circinelloides gene products and analyze their relevance in different biological processes. However, no information is available on M. circinelloides genes of stable expression that could serve as internal references in qRT-PCR analyses. One approach to solve this problem consists in the use of housekeeping genes as internal references. However, validation of the usability of these reference genes is a fundamental step prior to initiating qRT-PCR assays. This work evaluates expression of several constitutive genes by qRT-PCR throughout the morphological differentiation stages of M. circinelloides; our results indicate that tfc-1 and ef-1 are the most stable genes for qRT-PCR assays during differentiation studies and they are proposed as reference genes to carry out gene expression studies in this fungus.
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Incremental clinical value of a dedicated RT planning FDG PET-CT over staging PET-CT in non-small cell lung cancer
International Nuclear Information System (INIS)
Lin, P.; Som, S.; Vinod, S.; Lin, M.; Shon, I. H.
2009-01-01
Full text:Objectives: To evaluate whether FDG-PET performed for radiotherapy planning can detect disease progression, compared with staging PET. Methods: Thirteen patients underwent a planning PET-CT for curative RT ( R T-PET ) within eight weeks of a staging PET-CT for newly diagnosed NSCLC between 10/2007 and 1/2009. All studies were acquired on a Philips GXL PET-CT using the same protocols, except RT-PET is acquired on a RT flat bed. The images were interpreted by consensus readings of two physicians: location/number, visual grading (0-4:3> liver, 4>brain), max transverse diameter ( M ax D ) (tumour margin is delineated by a SUV threshold of 2.5) and max SUV of each lesion. Progressive disease (PD) is defined as >10% increase in max D. Results: RT-PET detected PD (primary or nodal) or new metastases in 8 pts (61%) (mean interval:30.2±14 days, range:7-54 days). For primary tumour, RT-PET detected PD in 5 pts (range: 12-32% increase in max D and 12-39% increase in SUV) and RT-CT detected PD in 3 pts (11-21% increase in max D, paired t test: p = 0.19). Stage-PET detected 28 mediastinal/hilar nodal sites. RT-PET detected PD in 11 of these lesions in 4 pts (31%) and CT detected similar progression in 8 lesions in 2 pts. RT-PET detected 10 new lesions in 3 pts (23%) resulting in upstaging to N3 in 2 pts (supraclavicular and hilar nodes) and M1 in 1 pt (bone). Conclusion: A dedicated RT PET-CT has the potential to detect disease progression and impact on RT planning in a large number of patients.
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Ecografía doppler ocular y retinopatía diabética. Correlación entre el índice de resistencia y severidad de la retinopatía
OpenAIRE
Montero Jaime, Manuel
2013-01-01
La diabetes mellitus (DM) es una patología que ha adquirido el rango de “pandemia” del siglo XXI .Actualmente unos 366 millones de personas sufren esta enfermedad a nivel mundial. Según datos de la encuesta de Salud de Cataluña (ESCA 2002) la prevalencia aproximada de la DM tipo 2 (DM2) en la población entre 30 – 69 años es del 6,1 % y en el intervalo poblacional entre 30 – 89 años puede llegar a superar el 10%. En el momento del diagnóstico de la DM tipo 2 el 5% de los pacientes presen...
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Comportamiento dinámico de asteroides en la resonancia 2:1
Science.gov (United States)
López García, F.; Brunini, A.
El propósito de este trabajo es mostrar los resultados obtenidos del estudio del movimiento de asteroides cercanos a la conmensurabilidad 2:1 con el movimiento medio de Júpiter, analizando el comportamiento de órbitas de asteroides reales y ficticios. Se analizan los tiempos de Lyapunov obtenidos a partir de la integración de las ecuaciones del movimiento. Se han estudiado la órbitas utilizando el modelo de cinco cuerpos y se han considerado los casos planar y espacial. Las simulaciones numéricas se realizaron utilizando integradores simpléticos y el intervalo de tiempo de integración fue de T = 107 años.
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Detection of canine distemper virus nucleocapsid protein gene in canine peripheral blood mononuclear cells by RT-PCR.
Science.gov (United States)
Shin, Y; Mori, T; Okita, M; Gemma, T; Kai, C; Mikami, T
1995-06-01
For a rapid diagnosis of canine distemper virus (CDV) infection, the reverse transcription-PCR (RT-PCR) was carried out to detect CDV nucleoprotein (NP) gene from canine peripheral blood mononuclear cells (PBMCs). Two sets of primers were targeted to two regions of NP gene of CDV Onderstepoort strain. The NP gene fragments were well amplified by the RT-PCR in each of the RNA extracts from Vero cells infected with 6 laboratory strains of CDV including Onderstepoort strain, and from PBMCs of a dog experimentally infected with CDV. The amplified NP gene was detected in 17 of 32 samples from dogs which were clinically suspected for CDV infection at veterinary hospitals. No RT-PCR product was found in 52 samples from healthy dogs including 40 specific pathogen free beagles vaccinated with an attenuated live virus-vaccine for CDV and 12 stray dogs. The RT-PCR provides a fast, sensitive, and supplementary method for the diagnosis of CDV infection in dogs.
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Evaluación de la respuesta a la tuberculina en estudiantes del área de la salud
Directory of Open Access Journals (Sweden)
María Patricia Arbeláez M.
2000-10-01
Full Text Available Se realizó un estudio transversal con el fin de evaluar la respuesta a la tuberculina en estudiantes del área de la salud (medicina, odontología, enfermería y bacteriología en comparación con estudiantes de otras áreas de la Universidad de Antioquia (Medellín, Colombia en tres niveles, (inicial, intermedio y final del programa académico. La muestra comprendió 490 estudiantes, 273 del área de la salud y 217 de las otras áreas; la selección se hizo de manera aleatoria con base en los listados del Departamento de Admisiones y Registros de la Universidad para el segundo semestre de 1998. Se determinó la presencia de cicatriz de vacunación BCG y factores de riesgo de tuberculosis. La tuberculina se evaluó 72 horas después de la aplicación intradérmica de 2 UT de PPD, RT-23. La respuesta a la tuberculina no mostró diferencias por nivel de estudios ni nivel socioeconómico; solo la presencia de cicatriz BCG estuvo significativamente asociada (P=0,007. Estos resultados indican que los estudiantes del área de la salud tienen un contacto reducido con pacientes con tuberculosis o sus muestras durante su formación, lo cual no descarta la tuberculosis como riesgo profesional para el personal de salud.
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Avaliação dos intervalos e números de observações no comportamento ingestivo de novilhas leiteiras confinadas Evaluation of the intervals and numbers of observations on the ingestive behavior of dairy heifers confined
Directory of Open Access Journals (Sweden)
Lucas Nascimento de Oliveira
2011-06-01
Full Text Available Objetivou-se verificar o efeito dos intervalos e números de observações no comportamento ingestivo de novilhas leiteiras confinadas. Foram utilizadas 20 novilhas mestiças Holandês x Zebu, com peso corporal médio inicial de 165,5kg e idade média de 13 meses. As novilhas foram distribuídas em quatro grupos, compostos por níveis de inclusão de farelo de cacau com base na matéria seca das dietas (0; 7; 14 e 21%. Os tratamentos do presente estudo foram os intervalos (cinco; 10; 15; 20 e 30 minutos, números de repetições (quatro, três, duas e uma, e turnos (quatro, três, dois e um de observação durante o comportamento ingestivo. A avaliação ocorreu no fim de cada período de 21 dias de coleta de dados, durante 24 horas, sendo as atividades registradas em cinco escalas diferentes: cinco; 10; 15; 20 e 30 minutos de intervalo. Os aspectos do comportamento ingestivo foram observados em quatro, três, duas e uma repetições e turnos. Não houve efeito significativo das escalas de registro sobre o tempo de alimentação, ruminação e ócio, nem sobre as eficiências de alimentação e ruminação. Para as variáveis relacionadas com a atividade de ruminação, não houve diferença entre os diferentes números de observações, nem para os diferentes turnos de observação. A determinação dos tempos totais das atividades de alimentação, ruminação e ócio, e as eficiências de alimentação e ruminação dos diferentes nutrientes podem ser realizadas com intervalo de observação de até 30 minutos.The objective of this study was to assess the effect of the intervals and numbers of observations on the ingestive behavior of confined dairy heifers. We used 20 heifers Holstein x Zebu, with initial body weight of 165.5kg and mean age of 13 months. The heifers were divided into four feeding groups, composed of inclusions of cocoa meal based diets (0; 7; 14 and 21%. The treatments were the intervals (five; 10; 15; 20 and 30 minutes
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Validación del método analítico para el control de la calidad y estudio de estabilidad de ketotifeno colirio 0,025 %
Directory of Open Access Journals (Sweden)
Yenilen Troche Concepción
Full Text Available El colirio de ketotifeno se indica para aliviar los signos y síntomas de las conjuntivitis alérgicas, por ser este un potente antihistamínico H1 que muestra cierta capacidad para inhibir la liberación de histamina y otros mediadores en mastocitos. En este trabajo se desarrolló y validó un método analítico por cromatografía líquida de alta resolución, para el control de la calidad y los estudios de estabilidad del ketotifeno colirio 0,025 %. El método se basó en la separación del principio activo a través una columna cromatográfica Lichrosorb RP-18 (5 µm (250 x 4 mm, con detección ultravioleta a 296 nm, para lo cual se empleó una fase móvil compuesta por una mezcla desgasificada de metanol:buffer fosfato (75:25; pH 8,5 y se le añadió 1 mL de isopropanol por cada 1 000 mL de la mezcla anterior, con una velocidad de flujo de 1,2 mL/min. El método analítico resultó lineal, preciso, específico y exacto en el intervalo de concentraciones estudiadas.
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Using the SPoRT POES/GOES Hybrid Product in OCONUS Forecasting
Science.gov (United States)
Smith, Matt; Fuell, Kevin; Nelson, Jim
2014-01-01
The SPoRT (Short-term Prediction and Research Transition) Program at the NASA/Marshall Space Flight Center has been providing unique NASA and NOAA data and techniques to partner Weather Forecast Offices (WFOs) for ten years. Data are provided in the Decision Support System used by WFO forecasters: AWIPS. For the last couple of years, SPoRT has been producing the POES/GOES Hybrid. This suite of products combines the strength ofl5- minute animations of GOES imagery - providing temporal continuity, with the higher resolution, relatively random availability, of polar orbiting (POES) imagery data. The product was first introduced with only MODIS data from NASA's Terra and Aqua satellites, but recently the VIIRS instrument onboard the Suomi-NPP satellite was added, providing better high-resolution coverage. These products represent SPoRT's efforts to prepare for higher resolution, higher frequency GOES-R imagery - as well as helping to move VIIRS (JPSS) data into the mainstream of weather forecasting. SPoRT generates 5 products for this dataset: Visible, Longwave Infrared (11 micrometers), Shortwave IR (3.7 micrometers), Water Vapor (6.7 micrometers), and Fog (Difference of 11 micrometer and 3.7 micrometer channels). The Water Vapor hybrid product has a Red-Blue-Green image from MODIS inlaid, since it provides even more qualitative information than water vapor alone. Animated examples of the products will be shown in this presentation. While the resolution at nadir of GOES imagery is nominally Han (4km for IR channels), the inlaid polar orbiter imagery has a resolution of 250m (lkm for IR channels). This has tremendous application in the continental US. However, in high latitudes, since the usefulness of GOES degrades poleward rapidly, the contrast of GOES and POES data is stark. The consistent temporal nature of GOES, even though at a reduced resolution at high latitudes, provides basic situational awareness, but the introduction of polar data is very helpful in seeing
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Transuranics and fission products release from PWR fuels in severe accident conditions. Lessons learnt from VERCORS RT3 and RT4 tests
International Nuclear Information System (INIS)
Pontillon, Y.; Ducros, G.; Van Winckel, S.; Christiansen, B.; Kissane, M.P.; Dubourg, R.; Dutheillet, Y.; Andreo, F.
2006-01-01
), chemical analysis of the filter deposits and other surface deposits downstream were performed in some specific cases, in order to extend the result to non gamma-active isotopes and confirm the gamma-spectroscopy results; this, in turn, will help to close the mass balance of the fission products and transuranic elements. In the present case, VERCORS RT3 and RT4 samples were sent to ITU in order to be analysed by Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) after dissolution. The present communication gives a general overview of the VERCORS program and presents in more detail the main findings regarding RT3 and RT4 with, on the one hand, the general FP behaviour including the comparison between gamma-spectrometry and ICP-MS results and, on the other hand, the significant release - up to ∼10 % of the initial inventory - of uranium in oxidizing conditions. (author)
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Changes in Plasma Prolactin and Growth Hormone Level and Visual Problem after radiation Therapy(RT) of Pituitary Adenoma
International Nuclear Information System (INIS)
Yoon, Sei Chul; Kwon, Hyung Chul; Oh, Yoon Kyeong; Bahk, Yong Whee; Son, Ho Young; Kang, Joon Ki; Song, Jin Un
1985-01-01
Twenty-four cases of pituitary adenoma, 13 males and 11 females with the age ranging from 11 to 65 years, received radiation therapy(RT) on the pituitary area with 6MV linear accelerator during past 25 months at the Division of Radiation Therapy, Kangnam St. Mary Hospital, Catholic Medical College. Of 24 case of RT, 20 were postoperative and 4 primary. To evaluate the effect of RT, we analyzed the alteration of the endocrinological tests, neurologic abnormalities, major clinical symptoms, endocrinological changes and improvement in visual problems after RT. The results were as follows ; 1. Major clinical symptoms were headache, visual defects, diabetes insipidus, hypogonadisms and general weakness in decreasing order of frequency. 2. All but the one with Nelson syndrome showed abnormal neuroradiologic changes in the sella turcica with an invasive tumor mass around supra and para-sellar area. 3. Endocrinological classifications of the patient were 11 prolactinoma, 4 growth hormonesecreting tumors, 3 ACTH-secreting tumors consisting of one Cushing disease and two Nelson syndrome, and 6 nonfunctioning tumors. 4. Eleven of 14 patients, visual problems were improved after treatment but remaining 3 were unchanged. 5. Seven of 11 prolactinomas returned to normal hormonal level after postoperative and primary RT and 3 patients are being treated with bromocriptine (BMCP) but on lost case. 6. Two of 4 growth hormone-secreting tumor returned to normal level after RT but the remaining 2 are being treated with BMCP, as well
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Historia del Pádel = History of Padel
Directory of Open Access Journals (Sweden)
Bernardino Javier Sánchez-Alcaraz Martínez
2013-10-01
Full Text Available El presente trabajo resume la historia del deporte del pádel. Existen dos versiones sobre el origen del pádel. La primera versión relaciona los antecedentes del pádel con los de otros deportes de raqueta como el tenis o el bádminton, siendo su origen común el jeu de la paume francés. Posteriormente, surgen variantes en Estados Unidos como el Paddle Tennis y el Platform Tennis, siendo versiones reducidas del tenis, pero con elementos muy comunes al pádel actual. La segunda versión, aprobada por la Federación Internacional de Pádel, afirma que este deporte nació en México, en 1969, a través del empresario Enrique Corcuera.--------------------------------------------------------------------This research summarizes the history of paddle sport. There are two versions about the origin of paddle. The first version relates the history of paddle with other racket sports like tennis or badminton, and its common origin of the Jeu de Paume French. Later, in in the United States, appear two sports called Paddle Tennis and Platform Tennis, which are reduced versions of tennis sport, but these have lot of common characteristics with paddle. The second version, approved by the International Padel Federation, said the sport was born in Mexico in 1969, through the businessman Enrique Corcuera.
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A simple RT-PCR-based strategy for screening connexin identity
Directory of Open Access Journals (Sweden)
M. Urban
1999-08-01
Full Text Available Vertebrate gap junctions are aggregates of transmembrane channels which are composed of connexin (Cx proteins encoded by at least fourteen distinct genes in mammals. Since the same Cx type can be expressed in different tissues and more than one Cx type can be expressed by the same cell, the thorough identification of which connexin is in which cell type and how connexin expression changes after experimental manipulation has become quite laborious. Here we describe an efficient, rapid and simple method by which connexin type(s can be identified in mammalian tissue and cultured cells using endonuclease cleavage of RT-PCR products generated from "multi primers" (sense primer, degenerate oligonucleotide corresponding to a region of the first extracellular domain; antisense primer, degenerate oligonucleotide complementary to the second extracellular domain that amplify the cytoplasmic loop regions of all known connexins except Cx36. In addition, we provide sequence information on RT-PCR primers used in our laboratory to screen individual connexins and predictions of extension of the "multi primer" method to several human connexins.
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Desempeño de la prueba de inmunofluorescencia directa en el diagnóstico del virus Influenza A(H1N1 Direct immunofluorescence assay performance in diagnosis of the Influenza A(H1N1 virus
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Luis Pianciola
2010-06-01
Full Text Available El 25 de abril de 2009, a menos de un mes de la detección en México del primer humano con virus Influenza A(H1N1, la enfermedad ya se había propagado a más de 40 países superando los 10 000 casos notificados. Dada su naturaleza impredecible, este tipo de virus requiere métodos diagnósticos apropiados, confiables y seguros, pero que también estén al alcance de los laboratorios clínicos. Mediante el estudio de 291 muestras de pacientes con sospecha de infección por virus Influenza A(H1N1 en Neuquén, Argentina, el presente trabajo compara los dos métodos de diagnóstico utilizados simultáneamente: la prueba de inmunofluorescencia directa (DFA y la de reacción en cadena de la polimerasa en tiempo real (RT-PCR. La DFA dio una sensibilidad de 44,4%, especificidad de 99,6%, valor predictivo positivo de 95,2% y valor predictivo negativo de 90,7%. Los resultados positivos de la metodología pueden considerarse verdaderos positivos. Un resultado negativo no excluye la presencia del virus y la muestra debe examinarse mediante RT-PCR. Del total de 291 muestras, 45 resultaron positivas por RT-PCR y 21 por DFA.By 25 April 2009, less than one month after the first human with Influenza A(H1N1 virus was detected in Mexico, the disease had already spread to more than 40 countries, with over 10 000 cases reported. Due to its unpredictability, this type of virus requires appropriate, reliable, and safe diagnostic methods that are also accessible to clinical laboratories. Through the analysis of 291 samples taken from patients with suspected Influenza A(H1N1 virus infection in Neuquén, Argentina, this study compares the two diagnostic methods used simultaneously: direct immunofluorescence assay (DFA and real-time polymerase chain reaction (RT-PCR. DFA had a sensitivity of 44.4%, a specificity of 99.6%, a positive predictive value of 95.2%, and a negative predictive value of 90.7%. Positive results obtained with this method can be considered true
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Factores predictores del inicio y consolidación del consumo de tabaco en adolescentes Analysis of factors related to smoking initiation and continued smoking in young adolescents
Directory of Open Access Journals (Sweden)
Araceli Caballero-Hidalgo
2005-12-01
Full Text Available Objetivo: Analizar los factores que determinan la adquisición y posterior consolidación del consumo de tabaco en los jóvenes adolescentes. Material y método: Estudio longitudinal de 3 años de duración (2000-2002 sobre jóvenes estudiantes de enseñanza obligatoria, con una edad al inicio del estudio de 13-14 años. Los datos fueron recogidos mediante encuesta individual, cuyo ámbito de estudio era la isla de Gran Canaria, con una muestra final de 745 individuos. Se estimaron modelos de elección binaria condicional de efectos fijos para datos longitudinales, para determinar la probabilidad de probar por primera vez el tabaco («modelo de inicio», así como sobre la probabilidad de ser fumador ocasional o habitual («modelo de experimentación». Resultados: En el último año, el 57% de los adolescentes encuestados ha tenido contacto con el tabaco alguna vez, un 25% más que en el primer año, algunos de ellos (el 9% de forma diaria. En el «modelo de inicio» los determinantes del consumo de tabaco son el interés por el colegio (odds ratio [OR] = 0,27; intervalo de confianza [IC] del 95%, 0,08-0,87, y OR = 0,14; IC del 95%, 0,03-0,58 para los que manifestaron bastante y mucho interés por el colegio, respectivamente, el consumo de tabaco por parte del mejor amigo (OR = 7,44; IC del 95%, 2,59-21,4, el consumo de alcohol (OR = 11,82; IC del 95%, 4,96-28,2, y OR = 15,42; IC del 95%, 4,68-50,7 para los que beben alcohol de manera ocasional o frecuente, respectivamente, y el dinero disponible (euros semanales (OR = 1,13; IC del 95%, 1,07-1,19. Para el «modelo de experimentación», el consumo de tabaco por parte del mejor amigo (OR = 7,01; IC del 95%, 2,96-16,5, el consumo de alcohol (OR = 5,71; IC del 95%, 1,98-16,4, y OR = 5,22; IC del 95%, 1,56-17,5 para los que beben alcohol de manera ocasional o frecuente, respectivamente y los años que lleva como fumador (OR = 1,44; IC del 95%, 1,11-1,86. Conclusiones: El estudio destaca
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CRISPR-RT: A web service for designing CRISPR-C2c2 crRNA with improved target specificity
OpenAIRE
Zhu, Houxiang; Richmond, Emily; Liang, Chun
2017-01-01
CRISPR-Cas systems have been successfully applied in genome editing. Recently, the CRISPR-C2c2 system has been reported as a tool for RNA editing. Here we describe CRISPR-RT (CRISPR RNA-Targeting), the first web service to help biologists design the crRNA with improved target specificity for the CRISPR-C2c2 system. CRISPR-RT allows users to set up a wide range of parameters, making it highly flexible for current and future research in CRISPR-based RNA editing. CRISPR-RT covers major model org...
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Análisis comparado del impacto de las políticas impositivas vía precio en el consumo de tabaco
Directory of Open Access Journals (Sweden)
Pinilla J.
2002-01-01
Full Text Available En este trabajo se analiza cómo el aumento de los impuestos afecta a la demanda de productos derivados del tabaco, en especial a la demanda de cigarrillos. El análisis comparado de los estudios revisados demuestra que las subidas en los impuestos sobre el tabaco se traducen en un aumento de los precios de estos productos. La elasticidad precio de la demanda de cigarrillos en países de ingreso medio y bajo resulta el doble que la de los países de ingresos altos, alrededor de -0,4. Además, y como señal de la naturaleza adictiva de este tipo de consumo, esta demanda se presenta más elástica en el largo que en el corto plazo. El efecto de una subida en los impuestos del tabaco es mayor en los jóvenes, más sensibles a los precios que los fumadores adultos. La evidencia empírica para el caso español sitúa la elasticidad precio de la demanda de cigarrillos a corto plazo en un intervalo que oscila entre -0,5 y -0,3, similar a los encontrados en la bibliografía internacional. Estos datos no ofrecen perspectivas optimistas sobre la potencialidad de las medidas fiscales como herramienta de control del tabaquismo, más allá de sus efectos recaudatorios y compensadores de externalidades. Además, si se consideran las posibilidades de sustituciones entre marcas y el margen estratégico de la industria para compensar los efectos de los impuestos, reducir las demandas y alentar el contrabando, el panorama se presenta todavía más pesimista.
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Un modelo de simulación estocástica para la estimación de escaños
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FÉLIX APARICIO
1987-01-01
Full Text Available El objeto del artículo es desarrollar un modelo teórico que permita realizar estimaciones de los escaños obtenidos por los distintos partidos y agrupaciones políticos en los procesos electorales. Estas estimaciones se basan en los resultados de las encuestas preelectorales. La principal ventaja del modelo es que se basa en una simulación estocástica y permite, por tanto, todo tipo de cálculos probabilísticas tales como intervalos de confianza, probabilidades condicionadas, etc.
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Productive fluid intervals in wells H-35 and H-39, Los Humeros geothermal field, Puebla (Mexico); Intervalos productores de fluidos en los pozos H-35 y H-39 del campo geotermico Los Humeros, Puebla, Mexico
Energy Technology Data Exchange (ETDEWEB)
Medina Martinez, Moises [Comision Federal de Electridad, Perote, Veracruz (Mexico)
2000-12-01
This paper presents the results of log interpretations using the percentage method to define the productive fluid intervals in wells H-35 and H-39. These were identified with pressure, temperature and flow rate (PTS) electronic logs, used for the first time in this field. The test were carried out with the well integrated to the electrical generation units. Well H-35 fed steam to Units 1, 4, 5 and 7 through a plate with a restriction orifice diameter of 50.8 mm (2 inches), a well head pressure of 45 bars and a mass flow rate of 40 t/h. Well H-39 fed steam to Unit 3 through plate with a restriction orifice diameter of 50.8 mm (2 inches), a well head pressure of 27 bars and a mass flow rate of 30 t/h. [Spanish] Se presenta la intervencion realizada por el metodo de porcentajes para definir los intervalos productores de fluidos en los pozos H-35 y H-39. El estudio se hizo a partir de datos obtenidos con una sonda electronica de presion, temperatura y velocidad de flujo (PTS), utilizada por primera vez en este campo. En ambos casos se realizaron las pruebas con el pozo integrado a las unidades de generacion electrica, el primero suministra vapor para las Unidades 1, 4, 5 y 7 a traves de una placa de orificio de restriccion de 50.8 mm (2 pulgadas) de diametro, con presion de cabezal de 45 bar manometricos y un gasto de mezcla de 40 t/h. El segundo alimenta a la Unidad 3, fluye por orificio de 50.8 mm (2 pulgadas) de diametro y produce 30 t/h de mezcla con una presion en la cabeza de 27 bar.
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Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
Directory of Open Access Journals (Sweden)
Isabel G. Fernández de Mera
2017-07-01
Full Text Available Crimean-Congo hemorrhagic fever (CCHF is an emerging tick-borne zoonotic disease caused by the CCHF virus (CCHFV. In this study, an experimental approach combining RT-PCR and proteomics was used for the identification and characterization of CCHFV in 106 ticks from 7 species that were collected from small ruminants in Greece. The methodological approach included an initial screening for CCHFV by RT-PCR followed by proteomics analysis of positive and control negative tick samples. This novel approach allowed the identification of CCHFV-positive ticks and provided additional information to corroborate the RT-PCR findings using a different approach. Two ticks, Dermacentor marginatus and Haemaphysalis parva collected from a goat and a sheep, respectively were positive for CCHFV. The sequences for CCHFV RNA segments S and L were characterized by RT-PCR and proteomics analysis of tick samples, respectively. These results showed the possibility of combining analyses at the RNA and protein levels using RT-PCR and proteomics for the characterization of CCHFV in ticks. The results supported that the CCHFV identified in ticks are genetic variants of the AP92 strain. Although the AP92-like strains probably do not represent a high risk of CCHF to the population, the circulation of genetically diverse CCHFV strains could potentially result in the appearance of novel viral genotypes with increased pathogenicity and fitness.
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Evaluating the Impacts of NASA/SPoRT Daily Greenness Vegetation Fraction on Land Surface Model and Numerical Weather Forecasts
Science.gov (United States)
Bell, Jordan R.; Case, Jonathan L.; Molthan, Andrew L.
2011-01-01
The NASA Short-term Prediction Research and Transition (SPoRT) Center develops new products and techniques that can be used in operational meteorology. The majority of these products are derived from NASA polar-orbiting satellite imagery from the Earth Observing System (EOS) platforms. One such product is a Greenness Vegetation Fraction (GVF) dataset, which is produced from Moderate Resolution Imaging Spectroradiometer (MODIS) data aboard the NASA EOS Aqua and Terra satellites. NASA SPoRT began generating daily real-time GVF composites at 1-km resolution over the Continental United States (CONUS) on 1 June 2010. The purpose of this study is to compare the National Centers for Environmental Prediction (NCEP) climatology GVF product (currently used in operational weather models) to the SPoRT-MODIS GVF during June to October 2010. The NASA Land Information System (LIS) was employed to study the impacts of the new SPoRT-MODIS GVF dataset on land surface models apart from a full numerical weather prediction (NWP) model. For the 2010 warm season, the SPoRT GVF in the western portion of the CONUS was generally higher than the NCEP climatology. The eastern CONUS GVF had variations both above and below the climatology during the period of study. These variations in GVF led to direct impacts on the rates of heating and evaporation from the land surface. The second phase of the project is to examine the impacts of the SPoRT GVF dataset on NWP using the Weather Research and Forecasting (WRF) model. Two separate WRF model simulations were made for individual severe weather case days using the NCEP GVF (control) and SPoRT GVF (experimental), with all other model parameters remaining the same. Based on the sensitivity results in these case studies, regions with higher GVF in the SPoRT model runs had higher evapotranspiration and lower direct surface heating, which typically resulted in lower (higher) predicted 2-m temperatures (2-m dewpoint temperatures). The opposite was true
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The SPoRT concept of bracing for idiopathic scoliosis.
Science.gov (United States)
Zaina, Fabio; Fusco, Claudia; Atanasio, Salvatore; Negrini, Stefano
2011-01-01
The SPoRT (acronym: Symmetrical, Patient-oriented, Rigid, Three-dimensional, active) concept of bracing is a new way to build braces based on our 20 years of experience and the biomechanical principles of scoliosis correction, inclusive of the Sibilla and Sforzesco braces. The concept always requires a custom brace, which is made according to the patient's individual requirements. New technologies such as CAD-CAM can be applied, and often for better results, without the customary use of prebuilt forms whose measurements are stored in databases. Once the initial draft brace is completed, a final test must be made on the patient to modify and adapt it, depending on his or her real interaction between the body and the brace. The results that are today available on the SPoRT concept relate to the Sforzesco brace and are necessarily short-term, because the first treated patients are now reaching the fourth-year follow-up examination and haven't yet completed their treatments. On the basis of the initial evaluations, we can state that the Sforzesco brace is more effective than the Lyon brace after 6 months of treatment and that the Sforzesco brace is equally effective as the Risser Plast brace.
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ANÁLISIS DEL RENDIMIENTO FÍSICO DE LOS ÁRBITROS DE FÚTBOL DURANTE PARTIDOS DE COMPETICIÓN OFICIAL
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J. Mallo
2010-09-01
Full Text Available RESUMEN El objetivo de este trabajo fue examinar la evolución de diversos parámetros cinemáticos y fisiológicos durante partidos de competición oficial en árbitros de fútbol. El análisis de la participación en el juego se realizó con un sistema fotogramétrico bidimensional durante 12 partidos del Campeonato del Mundo sub-17 del año 2003. La frecuencia cardiaca se registró con pulsómetros Polar S610 en intervalos de 5 s. Se pudo apreciar un descenso del rendimiento físico en distintos momentos del encuentro: (i tras los 5 min más intensos del partido en los siguientes 5 min disminuyó (p<0.05 la cantidad de ejercicio de alta intensidad realizado en relación a la media del partido; (ii en los primeros 15 min del segundo tiempo la distancia recorrida (p<0.05, el ejercicio de alta intensidad (p<0.01 y la FCmed (p<0.001 fueron inferiores a los valores de la fase inicial del partido; y (iii durante el segundo período se produjo una disminución global de la intensidad del esfuerzo. Los resultados de este estudio sugieren que se deben prescribir programas de entrenamiento específicos para los árbitros para prevenir, o cuanto menos retardar, esta pérdida de rendimiento físico en distintos momentos de un partido. PALABRAS CLAVE: análisis biomecánico 2D; distancia recorrida; ejercicio alta intensidad; frecuencia cardiaca. ABSTRACT The aim of this study was to examine the procedural knowledge level in young volleyball players and to establish the relationship between procedural knowledge, experiencie and performance, being the performance the championship positions obtained at the end of the competition. Participants in the study were 110 male young players of the regional teams
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Märt Sultsi kavandatud kasiinosaar vajab 300 miljonit krooni laenu / Kertu Kalmus
Index Scriptorium Estoniae
Kalmus, Kertu
2008-01-01
Tallinna linnavolikogu liige Märt Sults palub linnavalitsused toetada ja aktsepteerida SA Kasiinosaar laenu taotlemist Saksamaalt 300 mln. krooni ulatuses. Tema ideekavandi järgi kerkiksid Tallinnas Paljassaare lahte tehissaared: lastesaar, kultuurisaar ja kasiinosaar
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Searching for the best real-time RT-PCRs to detect Zika virus infections: the importance of comparing several protocols
Directory of Open Access Journals (Sweden)
F.M. de Moraes
2018-05-01
Full Text Available Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zika diagnosis is best achieved by real-time RT-PCR. However, some real-time RT-PCR show better performance than others. To reach the best possible Zika diagnosis, the analytic sensitivity of some probe-based real-time RT-PCR amplifying Zika virus RNA was evaluated in spiked and clinical samples. We evaluated primers and probes to detect Zika virus, which had been published before, and tested sensitivity using serum spiked and patient samples by real-time RT-PCR. When tested against spiked samples, the previously described primers showed different sensitivity, with very similar results when samples from patients (serum and urine were analyzed. Real-time RT-PCR designed to amplify Zika virus NS1 showed the best analytical sensitivity for all samples.
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Epidemiology of Human Parechovirus Type1 in Clinical Samples from Children with Gastroenteritis Using RT-PCR
Directory of Open Access Journals (Sweden)
Dabirmanesh B
2011-03-01
Full Text Available Background and Objectives: Human parechovirus type-1 (HPeV-1 is a genus of picornaviridea with a single stranded positive sense RNA genome. In general it seems to be responsible for more gastrointestinal and respiratory syndromes and less responsible for central nervous system (CNS symptoms. Since there is no accurate information about diagnosis and epidemiology of HPeV-1 in Iran and it is very important to distinguish between viral and bacterial diarrhea to decrease the unnecessary use of antibiotics, this study aimed at rapid detection and epidemiology of HPeV-1 in stool samples from children with gastroenteritis using specific RT-PCR. Methods: Viral RNA was isolated from 472 stool samples from children (under 4 years old with diarrhea; CDNA was prepared and amplified using specific primers from 5′untranslated region (5′ UTR of HPeV-1 genome by nested RT-PCR. Amplified DNA product was electrophoresed on 1% agarose gel and a single band of 265 bp was obtained. Data were analyzed by SPSS software. We also performed a comparison between the cell culture (Vero and RT-PCR method for HPeV1 detection.Results: Out of 472 samples examined during two years, 112 samples were HpeV-1 positive (23.7%. The results showed that the prevalence of this virus was in children under one year (6-12 months old with diarrhea (p=0.036 in spring and autumn (p<0.001. Boys had more positive cases than the girls (p<0.001. Out of 20 samples which were found positive by HPeV1 RT-PCR only three of them showed CPE on Vero Cells after a week.Conclusion: The results revealed that RT-PCR is a more practical and sensitive technique for HPeV-1 detection directly from clinical samples, which is valuable for epidemiology. Also, the rapid detection of HPeV1 by RT-PCR can decrease both the unnecessary use of antibiotics and the costs in clinical practice
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Potencial del Agua del suelo
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Bustamante Heliodoro
1986-12-01
Full Text Available La energía potencial del agua presenta diferencias de un punto del suelo a otro; esas diferencias son las que originan el movimiento del agua de acuerdo a la tendencia universal de la materia en el sentido de moverse de donde la energía potencial es mayor a donde dicha energía es menor. En el suelo el agua en consecuencia se mueve hacia donde su energía decrece hasta lograr su estado de equilibrio. Se desprende entonces que la cantidad de energía potencial absoluta contenida en el agua, no es importante por sí misma, sino por su relación con la energía en diferentes lugares dentro del suelo. El concepto Potencial de agua del suelo es un criterio para esta energía.
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Dynamo transformation of the collisional R-T in a weakly ionized ...
Indian Academy of Sciences (India)
where the interstellar neutrals undergo charge exchange collisions with ions in the solar wind [1]. ... regime of magnetically confined torroidal plasma. The author refutes their ... rate ´7К-М µ for the usual collisional R-T is given as. 7К-М g v Дp.
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Evaluación del ruido en la unidad de cuidados intensivos neonatal
Directory of Open Access Journals (Sweden)
Alessandra Teixeira
2011-12-01
Full Text Available Introducción: Con los avances en la tecnología y la investigación llevada a cabo en neonatología, ya es posible ver más allá de lo que podamos para salvaguardar las vidas de recién nacidos (RN, proporcionando una calidad de vida. Para esto necesitamos tener una visión amplia que se ve más allá del individuo, el medio ambiente y el equipo que le rodea. Humanización debe impregnar todas las acciones tomadas en la Unidad de Cuidados Intensivos Neonatales (UCIN. Objetivo: En este contexto, el ruido ambiental emerge como un objeto de estudio de esta investigación tiene como objetivo identificar los niveles de ruido a que están expuestos los recién nacidos ingresados en una unidad específica de cuidados intensivos neonatales y discutirlos a la luz de las normas vigentes país. Materiales y Métodos: Teniendo en cuenta el tema de la parte delantera de esta investigación considera que es importante conocer la legislación que aborda este tema, específicamente el procedimiento estándar de Higiene Profesional Técnica (NHO 01 y la norma 15 (NR15. De acuerdo con la Norma 15 (NR 15 promulgado por el Ministerio de la Ordenanza N º 3214/1978 del Trabajo, actualizado por el Decreto 203/2011, el ruido que se clasifica como el impacto del ruido o el ruido continuo también llamado intermitente, que se caracteriza los picos de energía acústica de menos de 1 (un segundo a intervalos superiores a 1 (un segundo. Resultados: Comenzamos la presentación de los resultados que describen el campo de estudio, los resultados de la investigación discutidos a la luz de las normas vigentes en el país. Discusión y Conclusiones: La institución por área de estudio es un centro de referencia para cuidados intensivos neonatales de la micro-región de Barbacena recibe pacientes de varios lugares. De acuerdo con la Resolución del Directorio N º 7 (RDC 07 se clasifica como un nivel de unidad de cuidadosintensivos II(10. (Rev Cuid 2011; 2
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A high-throughput method to detect RNA profiling by integration of RT-MLPA with next generation sequencing technology.
Science.gov (United States)
Wang, Jing; Yang, Xue; Chen, Haofeng; Wang, Xuewei; Wang, Xiangyu; Fang, Yi; Jia, Zhenyu; Gao, Jidong
2017-07-11
RNA in formalin-fixed and paraffin-embedded (FFPE) tissues provides large amount of information indicating disease stages, histological tumor types and grades, as well as clinical outcomes. However, Detection of RNA expression levels in formalin-fixed and paraffin-embedded samples is extremely difficult due to poor RNA quality. Here we developed a high-throughput method, Reverse Transcription-Multiple Ligation-dependent Probe Sequencing (RT-MLPSeq), to determine expression levels of multiple transcripts in FFPE samples. By combining Reverse Transcription-Multiple Ligation-dependent Amplification method and next generation sequencing technology, RT-MLPSeq overcomes the limit of probe length in multiplex ligation-dependent probe amplification assay and thus could detect expression levels of transcripts without quantitative limitations. We proved that different RT-MLPSeq probes targeting on the same transcripts have highly consistent results and the starting RNA/cDNA input could be as little as 1 ng. RT-MLPSeq also presented consistent relative RNA levels of selected 13 genes with reverse transcription quantitative PCR. Finally, we demonstrated the application of the new RT-MLPSeq method by measuring the mRNA expression levels of 21 genes which can be used for accurate calculation of the breast cancer recurrence score - an index that has been widely used for managing breast cancer patients.
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Quantitative real-time RT-PCR and chromogenic in situ hybridization: precise methods to detect HER-2 status in breast carcinoma
International Nuclear Information System (INIS)
Rosa, Fabíola E; Silveira, Sara M; Silveira, Cássia GT; Bérgamo, Nádia A; Neto, Francisco A Moraes; Domingues, Maria AC; Soares, Fernando A; Caldeira, José RF; Rogatto, Silvia R
2009-01-01
HER-2 gene testing has become an integral part of breast cancer patient diagnosis. The most commonly used assay in the clinical setting for evaluating HER-2 status is immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). These procedures permit correlation between HER-2 expression and morphological features. However, FISH signals are labile and fade over time, making post-revision of the tumor difficult. CISH (chromogenic in situ hybridization) is an alternative procedure, with certain advantages, although still limited as a diagnostic tool in breast carcinomas. To elucidate the molecular profile of HER-2 status, mRNA and protein expression in 75 invasive breast carcinomas were analyzed by real time quantitative RT-PCR (qRT-PCR) and IHC, respectively. Amplifications were evaluated in 43 of these cases by CISH and in 11 by FISH. The concordance rate between IHC and qRT-PCR results was 78.9%, and 94.6% for qRT-PCR and CISH. Intratumoral heterogeneity of HER-2 status was identified in three cases by CISH. The results of the three procedures were compared and showed a concordance rate of 83.8%; higher discordances were observed in 0 or 1+ immunostaining cases, which showed high-level amplification (15.4%) and HER-2 transcript overexpression (20%). Moreover, 2+ immunostaining cases presented nonamplified status (50%) by CISH and HER-2 downexpression (38.5%) by qRT-PCR. In general, concordance occurred between qRT-PCR and CISH results. A high concordance was observed between CISH/qRT-PCR and FISH. Comparisons with clinicopathological data revealed a significant association between HER-2 downexpression and the involvement of less than four lymph nodes (P = 0.0350). Based on these findings, qRT-PCR was more precise and reproducible than IHC. Furthermore, CISH was revealed as an alternative and useful procedure for investigating amplifications involving the HER-2 gene
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Efecto del silicio y plaguicidas en la fertilidad del suelo y rendimiento del arroz
OpenAIRE
Furcal-Beriguete, Parménides; Herrera-Barrantes, Alejandra
2013-01-01
Efecto del silicio y plaguicidas en la fertilidad del suelo y rendimiento del arroz. El objetivo de este trabajo fue evaluar el efecto del silicio en la fertilidad del suelo, la incidencia de enfermedades y plagas insectiles, el rendimiento y la calidad de granos del cultivo de arroz. El estudio se realizó en La Vega, Florencia, San Carlos, Costa Rica entre mayo y octubre en los años 2010 y 2011, en el mismo lote con la variedad CR 4477. Se establecieron cinco tratamientos: silicio al suelo, ...
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Evaluación de la de vigilancia epidemiológia de la brucelosis en la Comunidad Autónoma del Pais Vasco
Directory of Open Access Journals (Sweden)
Coll Jordá D.
1997-01-01
Full Text Available FUNDAMENTO: Se evalúa el Sistema de Vigilancia Epidemiologíca de las Enfermedades de Declaración Obligatoria, por medio de la brucelosis, que es la zoonosis de mayor incidencia en todo el Estado, y en la Comunidad Autónoma del País Vasco es de notificación individualizada. MÉTODOS: Se analizó la información del Sistema y sus usos y utilidades. Se realizó una búsqueda activa de casos en el Sistema de Información Microbiológico (SIM, en los Hospitales de titularidad pública, y en el Registro de Enfermedades Profesionales. Se compararon los datos de la notificación y la búsqueda. Y por el método de captura recaptuara se compararon los casos de SVE-EDO y hospitales. RESULTADOS: Se detectan riesgos y brotes. La notificación no se realiza bajo sospecha. El aumento de casos después de la búsqueda activa fue de un 40%, 24 frente a 40. La Sensibilidad se estimó en un 60%, el valor predictivo positivo no se pudo calcular. No existe definición de caso. La sensibilidad conjunta, estimada por el método de captura y recaptura, de SVE-EDO y la información hospitalaria es del 84% (Intervalo Confianza 95% 70-100. El número estimado de casos por este método fue de 38+8. DISCUSIÓN: Hay una subdeclaración y como consecuencia una baja sensibilidad. Es necesaria una definición de caso. Notificar después de la confirmación retrasa el tiempo de actuación. El número de casos encontrados en la búsqueda activa es compatible con el calculado por el método de captura-recaptura con solo dos fuentes.
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Variação da evapotranspiração de referência calculada em diferentes intervalos de tempo Variation of the reference evapotranspiration calculated for different times interval
Directory of Open Access Journals (Sweden)
Álvaro J. Back
2007-04-01
Full Text Available Valores de evapotranspiração de referência foram calculados em intervalos de um, cinco, dez, quinze dias e mensal. Foram utilizados dados meteorológicos da Estação Meteorológica de Urussanga - SC (latitude 28°31'S, longitude 49°19'W referentes ao período de 1981 a 2004. A evapotranspiração de referência foi calculada pelo método de Penman-Monteith com base nas médias das variáveis meteorológicas dos diferentes períodos estudados. Observou-se que não houve diferenças significativas nas médias de evapotranspiração calculadas nos diversos intervalos de tempo. No entanto, houve diferenças significativas na dispersão dos valores em torno da média. Para valores diários, foi obtido coeficiente de variação (C.V. entre 30 e 40%, e para valores mensais, variou entre 6% e 12%. Observou-se, também, que os dados de evapotranspiração se ajustaram à distribuição Beta, e assim foram estimados, para cada mês, os valores de evapotranspiração de referência com probabilidades de 5; 10; 25; 50; 75; 90 e 95%.Reference evapotranspiration values were calculated for time interval of one, five, ten, fifteen days and monthly. Meteorological series data from 1981 to 2004 from Urussanga Weather Station (latitude 28°31'S, longitude 49°19'W were used to calculate reference evapotranspiration with Penman-Monteith method with mean of meteorological variables for every time intervals. No difference was observed in average reference evapotranspiration computed in all time steps. However, some difference in variations of data around the average was observed. For daily value, the variation coefficient varies from 30 to 40% and for monthly values de coefficient variation varied from 6 to 12%. It was observed that the reference evapotranspirations adjusted very well to Beta distribution probability, and the reference evapotranspiration with probability of 5; 10; 25; 50; 75; 90 e 95% was estimated.
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Factores de riesgo que influyen en el retardo del brote de la dentición temporal.: Policlínico "Turcios Lima", 2000-2003 Risk factors influencing the primary dentition eruption retardation. "Turcios Lima" polyclinics, 2000-2003
Directory of Open Access Journals (Sweden)
Zoila Rosa Podadera Valdés
2004-04-01
Full Text Available Se realizó un estudio analítico de casos y controles en el área de salud del Policlínico "Turcios Lima" en el período comprendido entre julio de 2000 y julio de 2003, con el objetivo de identificar los factores de riesgo que influyen en el retardo del brote de la dentición temporal. El universo estuvo constituido por 1454 niños, de los cuales se obtuvo una muestra aleatoria de 93 niños. El análisis estadístico se realizó mediante porcentajes, la prueba Chi cuadrado y el cálculo de los odds ratio con sus intervalos de confianza. Se observó que el estado nutricional anormal al inicio del embarazo, las enfermedades asociadas con este, la inadecuada ganancia de peso de la madre y el desarrollo físico del niño, constituyeron los factores que más influyeron en la problemática estudiada. Se concluye que existe una significativa influencia del estado nutricional de la madre y del niño en el retardo del brote de su dentición temporal.An analytical case-control study was carried out in "Turcios Lima" polyclinics from July, 2000 to July, 2003, to identify the risk factors influencing primary dentition eruption retardation. The universe was formed by 1 454 children from whom a random sample of 93 children was taken. The statistical analysis was based on Chi-square test and calculation of percentages and odds ratios with confidence intervals. It was observed that poor nutritional status at the beginning of pregnancy, pregnancy-related diseases, inadequate weight gain by the mother and the physical development of the infant were the most influential factors. It was concluded that there is significant influence of the nutritional status of the child and the mother on primary dentition eruption retardation.
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Elu on väärt riskideski / Juta Üts
Index Scriptorium Estoniae
Üts, Juta, 1934-
2004-01-01
Tallinna Noorte Naiste ja Noorte Meeste Kristlik Ühing aitab läbi projektide "Terve Eesti laps" ja "Elu on väärt ka riskides" õpetada astma- ja suhkruhaigetele lastele toimetulekut oma haigusega. Projekte tutvustavad Tallinna NNKÜ ja NMKÜ peasekretär Katrin Ohno, Tallinna Kesklinna Lastepolikliiniku päevastatsionaari töötaja Viive Sirge ning endokrinoloog dr. Riina Shor
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Assessment of brain reference genes for RT-qPCR studies in neurodegenerative diseases.
Science.gov (United States)
Rydbirk, Rasmus; Folke, Jonas; Winge, Kristian; Aznar, Susana; Pakkenberg, Bente; Brudek, Tomasz
2016-11-17
Evaluation of gene expression levels by reverse transcription quantitative real-time PCR (RT-qPCR) has for many years been the favourite approach for discovering disease-associated alterations. Normalization of results to stably expressed reference genes (RGs) is pivotal to obtain reliable results. This is especially important in relation to neurodegenerative diseases where disease-related structural changes may affect the most commonly used RGs. We analysed 15 candidate RGs in 98 brain samples from two brain regions from Alzheimer's disease (AD), Parkinson's disease (PD), Multiple System Atrophy, and Progressive Supranuclear Palsy patients. Using RefFinder, a web-based tool for evaluating RG stability, we identified the most stable RGs to be UBE2D2, CYC1, and RPL13 which we recommend for future RT-qPCR studies on human brain tissue from these patients. None of the investigated genes were affected by experimental variables such as RIN, PMI, or age. Findings were further validated by expression analyses of a target gene GSK3B, known to be affected by AD and PD. We obtained high variations in GSK3B levels when contrasting the results using different sets of common RG underlining the importance of a priori validation of RGs for RT-qPCR studies.
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Voldemar Panso preemia said kaksikvennad Priit ja Märt Pius / Tiiu Laks
Index Scriptorium Estoniae
Laks, Tiiu, 1984-
2010-01-01
Lavakunstikooli 25. lennu üliõpilased Priit ja Märt Pius pälvisid Voldemar Panso nimelise preemia. Terve kursuse esimene bakalaureuselavastus "Jumala Narride Vennaskond" esietendub 4. detsembril Tallinna Linnateatri Põrgulaval. Lavastas kursuse juhendaja Elmo Nüganen
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Las abejas (Hymenoptera: Apoidea del Parque Nacional Natural Gorgona, Pacífico colombiano
Directory of Open Access Journals (Sweden)
Marcela González-Córdoba
2014-02-01
Full Text Available En este estudio se presenta la diversidad y abundancia de las abejas del PNN Gorgona. En tres visitas en intervalos de tres meses, se muestrearon seis zonas de la isla con cubierta vegetal variable. Los muestreos fueron indirectos usando (i trampas McPhail (cebadas con cineol, silicato de metilo y extracto clavos; (ii trampas Van Someren Rydon (con cebo de pescado o pollo o fruta podridos, (iii trampas Malaise y (iv trampas de luz y directos a lo largo de transectos de longitud indefinida, por períodos de una hora en cada área y se repitieron cuatro veces. Un total de 585 especímenes fueron capturados, de ellos, 443 pertenecen a Apidae (14 géneros, 141 a Halictidae (seis géneros y uno a Megachilidae. Con este informe, el inventario local de Abejas se eleva a 43 especies y 28 géneros. Trigona fulviventris guianae Cockerell, 1910 fue la especie más abundante, Euglossa ignita Smith, 1874 la más atraída por los cebos aromáticos y Megalopta genalis Smith, 1853 dominó durante la noche. Apis mellifera Linnaeus, 1758 no se encontró en los muestreos. La importancia de la Isla Gorgona, como sitio de la diversidad para las especies de abejas es discutida.
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A methodology for improving the SIS-RT in analyzing the traceability of the documents written in Korean language
International Nuclear Information System (INIS)
Yoo, Yeong Jae; Kim, Man Cheol; Seong, Poong Hyun
2002-01-01
Inspection is widely believed to be an effective software verification and validation (V and V) method. However, software inspection is labor-intensive. This labor-intensive nature is compounded by a view that since software inspection uses little technology, they do not fit in well with a more technology-oriented development environment. Nevertheless, software inspection is gaining in popularity. The researchers of KAIST I and C laboratory developed the software tool managing and supporting inspection tasks, named SIS-RT. SIS-RT is designed to partially automate the software inspection processes. SIS-RT supports the analyses of traceability between the spec documents. To make SIS-RT prepared for the spec document written in Korean language, certain techniques in natural language processing have been reviewed. Among those, the case grammar is most suitable for the analyses of Korean language. In this paper, the methodology for analyzing the traceability between spec documents written in Korean language will be proposed based on the case grammar
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Evaluation of NASA SPoRT's Pseudo-Geostationary Lightning Mapper Products in the 2011 Spring Program
Science.gov (United States)
Stano, Geoffrey T.; Carcione, Brian; Siewert, Christopher; Kuhlman, Kristin M.
2012-01-01
NASA's Short-term Prediction Research and Transition (SPoRT) program is a contributing partner with the GOES-R Proving Ground (PG) preparing forecasters to understand and utilize the unique products that will be available in the GOES-R era. This presentation emphasizes SPoRT s actions to prepare the end user community for the Geostationary Lightning Mapper (GLM). This preparation is a collaborative effort with SPoRT's National Weather Service partners, the National Severe Storms Laboratory (NSSL), and the Hazardous Weather Testbed s Spring Program. SPoRT continues to use its effective paradigm of matching capabilities to forecast problems through collaborations with our end users and working with the developers at NSSL to create effective evaluations and visualizations. Furthermore, SPoRT continues to develop software plug-ins so that these products will be available to forecasters in their own decision support system, AWIPS and eventually AWIPS II. In 2009, the SPoRT program developed the original pseudo geostationary lightning mapper (PGLM) flash extent product to demonstrate what forecasters may see with GLM. The PGLM replaced the previous GLM product and serves as a stepping-stone until the AWG s official GLM proxy is ready. The PGLM algorithm is simple and can be applied to any ground-based total lightning network. For 2011, the PGLM used observations from four ground-based networks (North Alabama, Kennedy Space Center, Oklahoma, and Washington D.C.). While the PGLM is not a true proxy product, it is intended as a tool to train forecasters about total lightning as well as foster discussions on product visualizations and incorporating GLM-resolution data into forecast operations. The PGLM has been used in 2010 and 2011 and is likely to remain the primary lightning training tool for the GOES-R program for the near future. This presentation will emphasize the feedback received during the 2011 Spring Program. This will discuss several topics. Based on feedback
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Evaluating the Impacts of NASA/SPoRT Daily Greenness Vegetation Fraction on Land Surface Model and Numerical Weather Forecasts
Science.gov (United States)
Bell, Jordan R.; Case, Jonathan L.; LaFontaine, Frank J.; Kumar, Sujay V.
2012-01-01
The NASA Short-term Prediction Research and Transition (SPoRT) Center has developed a Greenness Vegetation Fraction (GVF) dataset, which is updated daily using swaths of Normalized Difference Vegetation Index data from the Moderate Resolution Imaging Spectroradiometer (MODIS) data aboard the NASA EOS Aqua and Terra satellites. NASA SPoRT began generating daily real-time GVF composites at 1-km resolution over the Continental United States (CONUS) on 1 June 2010. The purpose of this study is to compare the National Centers for Environmental Prediction (NCEP) climatology GVF product (currently used in operational weather models) to the SPoRT-MODIS GVF during June to October 2010. The NASA Land Information System (LIS) was employed to study the impacts of the SPoRT-MODIS GVF dataset on a land surface model (LSM) apart from a full numerical weather prediction (NWP) model. For the 2010 warm season, the SPoRT GVF in the western portion of the CONUS was generally higher than the NCEP climatology. The eastern CONUS GVF had variations both above and below the climatology during the period of study. These variations in GVF led to direct impacts on the rates of heating and evaporation from the land surface. In the West, higher latent heat fluxes prevailed, which enhanced the rates of evapotranspiration and soil moisture depletion in the LSM. By late Summer and Autumn, both the average sensible and latent heat fluxes increased in the West as a result of the more rapid soil drying and higher coverage of GVF. The impacts of the SPoRT GVF dataset on NWP was also examined for a single severe weather case study using the Weather Research and Forecasting (WRF) model. Two separate coupled LIS/WRF model simulations were made for the 17 July 2010 severe weather event in the Upper Midwest using the NCEP and SPoRT GVFs, with all other model parameters remaining the same. Based on the sensitivity results, regions with higher GVF in the SPoRT model runs had higher evapotranspiration and
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Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus.
Science.gov (United States)
Santiago, Gilberto A; Vergne, Edgardo; Quiles, Yashira; Cosme, Joan; Vazquez, Jesus; Medina, Juan F; Medina, Freddy; Colón, Candimar; Margolis, Harold; Muñoz-Jordán, Jorge L
2013-01-01
Dengue is an acute illness caused by the positive-strand RNA dengue virus (DENV). There are four genetically distinct DENVs (DENV-1-4) that cause disease in tropical and subtropical countries. Most patients are viremic when they present with symptoms; therefore, RT-PCR has been increasingly used in dengue diagnosis. The CDC DENV-1-4 RT-PCR Assay has been developed as an in-vitro diagnostic platform and was recently approved by the US Food and Drug Administration (FDA) for detection of dengue in patients with signs or symptoms of mild or severe dengue. The primers and probes of this test have been designed to detect currently circulating strains of DENV-1-4 from around the world at comparable sensitivity. In a retrospective study with 102 dengue cases confirmed by IgM anti-DENV seroconversion in the convalescent sample, the RT-PCR Assay detected DENV RNA in 98.04% of the paired acute samples. Using sequencing as a positive indicator, the RT-PCR Assay had a 97.92% positive agreement in 86 suspected dengue patients with a single acute serum sample. After extensive validations, the RT-PCR Assay performance was highly reproducible when evaluated across three independent testing sites, did not produce false positive results for etiologic agents of other febrile illnesses, and was not affected by pathological levels of potentially interfering biomolecules. These results indicate that the CDC DENV-1-4 RT-PCR Assay provides a reliable diagnostic platform capable for confirming dengue in suspected cases.
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Avaliação da idade materna, paterna, ordem de paridade e intervalo interpartal para fissura lábio-palatina Maternal and paternal age, birth order and interpregnancy interval evaluation for cleft lip-palate
Directory of Open Access Journals (Sweden)
Daniella Reis Barbosa Martelli
2010-02-01
Full Text Available Fissuras do lábio e/ou palato representam as anomalias congênitas craniofaciais mais comuns. OBJETIVO: Avaliar fatores de risco ambientais em pacientes com fissuras lábio-palatinas não-sindrômicas, em um Serviço de Minas Gerais. CASUÍSTICA E MÉTODO: Realizou-se estudo caso-controle, avaliando 100 crianças com fissuras e 100 crianças sem alterações clínicas. As dimensões de análise (idade, cor de pele, sexo, classificação das fissuras, idade materna e paterna, ordem de paridade e intervalo interpartal foram obtidas a partir de um questionário, sendo posteriormente construído banco de dados e as análises realizadas pelo programa SPSS 17.0. Os resultados foram analisados com risco relativo para cada variável, para estimar odds ratios com intervalo de confiança de 95% seguido de análise bivariada e multivariada. RESULTADOS: Entre as 200 crianças, 54% foram do sexo masculino e 46% do feminino. Com relação à cor da pele, houve predomínio de parda, branca e preta, respectivamente. Entre os tipos de fissuras, as mais comuns foram as fissuras lábio-palatinas (54%, seguidas pela fissura labial (30% e fissura palatina (16%. CONCLUSÃO: Embora com uma população limitada, verificou-se associação entre idade materna e risco aumentado para fissuras lábio-palatinas, porém idade paterna, ordem de paridade e intervalo interpartal não foram significantes.Cleft lip and palate (CL/P are the most common congenital craniofacial anomalies. AIM: To evaluate environmental risk factors for non-syndromic CL/P in a reference care center in Minas Gerais. MATERIALS AND METHODS: we carried out a case-controlled study, assessing 100 children with clefts and 100 children without clinical alterations. The analysis dimensions (age, skin color, gender, fissure classification, maternal and paternal age, birth order and interpregnancy interval, obtained from a questionnaire; and later we build a data base and the analyses were carried out by the
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Läheneb Vikerkaare 75. aastapäev / Märt Väljataga
Index Scriptorium Estoniae
Väljataga, Märt, 1965-
1997-01-01
Vastukaja : Haug, Toomas. Kultuurilooline õppetund peatoimetaja Väljatagale. // Eesti Ekspress (1997) 18. apr., lk. B6. (Poleemika); Väljataga, Märt. Vikerkaart nähti juba Sumeris!. // Eesti Ekspress (1997) 25. apr., lk. B7; Haug, Toomas. Ei soovi vasikaga võidu joosta. // Eesti Ekspress (1997) 2. mai, lk. B7
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Mesing Kirke:Opmålinger og undersøgelser af Mesing Kirke udført 1978
OpenAIRE
Bock, Lars Nicolai; Longmose Jakobsen, Bendt; Nielsen, Ole
1989-01-01
Detaljerede opmålinger og bygningsarkæologiske undersøgelser udført i Mesing Kirke i efteråret 1978. Arbejdet er udført i samarbejde med Bendt Longmose Jakobsen og Ole Nielsen. Det samlede arbejde indgår i Nationalmuseets værk Danmarks Kirker, XVI, bind 6 (1988-89) Side: 3123 - 3147. Afsnittet om Mesing Kirke er skrevet og redigeret af dr. arkitekt maa Kjeld de Fine Licht
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Simultaneous VENTANA IHC and RT-PCR testing of ALK status in Chinese non-small cell lung cancer patients and response to crizotinib.
Science.gov (United States)
Xu, Chun-Wei; Wang, Wen-Xian; Chen, Yan-Ping; Chen, Yu; Liu, Wei; Zhong, Li-Hua; Chen, Fang-Fang; Zhuang, Wu; Song, Zheng-Bo; Chen, Xiao-Hui; Huang, Yun-Jian; Guan, Yan-Fang; Yi, Xin; Lv, Tang-Feng; Zhu, Wei-Feng; Lu, Jian-Ping; Wang, Xiao-Jiang; Shi, Yi; Lin, Xian-Dong; Chen, Gang; Song, Yong
2018-04-11
ALK rearrangement-advanced NSCLC patients respond to crizotinib. ALK rearrangement is currently determined with RT-PCR. VENTANA IHC is a standard method to identify ALK protein overexpression in NSCLC; however, VENTANA IHC has rarely been used to determine the response to crizotinib in Chinese patients with NSCLC and ALK overexpression. To better clarify the clinical implication of VENTANA IHC to detect ALK rearrangements, we conducted this study to analyze VENTANA IHC and RT-PCR in a large cohort of Chinese patients with NSCLC undergoing screening for ALK rearrangements. A total of 1720 patients with NSCLC who had ALK rearrangements detected by VENTANA IHC and/or RT-PCR were included in this analysis. We compared the efficacy and survival of ALK-positive patients detected by VENTANA IHC and RT-PCR. We used NGS to identify patients in whom the two methods were inconsistent. Among 1720 patients, 187 (10.87%) were shown to be ALK-positive by VENTANA IHC and/or RT-PCR, and 66 received crizotinib treatment. We identified 10.27% (172/1674) of patients as ALK-positive by the VENTANA IHC method, and 12.73% (41/322) of patients had ALK rearrangements by the RT-PCR method. Twenty-nine of 276 (10.51%) ALK-positive patients were simultaneously analyzed using VENTANA IHC and RT-PCR. The overall response rates were 65.90% (29/44) by VENTANA IHC and 55.88% (19/34) by RT-PCR. The disease control rates were 86.36% (38/44) by VENTANA IHC and 76.47% (26/34) by RT-PCR. In contrast, the median progression-free survival for VENTANA IHC and RT-PCR was 8.5 and 9.2 months, respectively. The VENTANA IHC and RT-PCR results obtained for 6 of 17 ALK-positive patients were inconsistent based on NGS; specifically, 4 patients had EML4-ALK fusions, 2 patients had non EML4-ALK fusions, 1 patient had a KCL1-ALK fusion, and one patient had a FBXO36-ALK fusion. VENTANA IHC is a reliable and rapid screening tool used in routine pathologic laboratories for the identification of suitable candidates for
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An Evidence-Based Approach to Detection by DASI-ELISA and RT-PCR in Dormant Period
Directory of Open Access Journals (Sweden)
Antonio Olmos
2008-01-01
Full Text Available An evidence-based approach, such as those developed in clinical and veterinary medicine, was applied to the detection of Plum pox virus (PPV during the dormant period. A standardized methodology was used for the calculation of parameters of the operational capacity of DASI-ELISA and RT-PCR in wintertime. These methods are routinely handled to test the sanitary status of plants in national or international trading and in those cases concerning export-import of plant materials. Diagnosis often has to be performed during the dormant period, when plant material is commercialized. Some guidelines to interpret diagnostic results of wintertime are provided in an attempt to minimize risks associated with the methods and over-reliance on the binary outcome of a single assay. In order to evaluate if a complementary test increased the confidence of PPV diagnosis when discordant results between DASI-ELISA and RT-PCR are obtained, NASBA-FH also was included. Likelihood ratios of each method were estimated based on the sensitivity and specificity obtained in wintertime. Subsequently, a Bayesian approach was performed to calculate post-test probability of PPV infection in spring. Results of evidence-based approach show that different PPV prevalences require different screening tests. Thus, at very low PPV prevalence levels DASI-ELISA should be used as the election method, whilst at the highest PPV prevalence levels RT-PCR should be performed. NASBA-FH could be used at medium prevalences to clarify discordances between DASI-ELISA and RT-PCR.
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Diagnostic evaluation of a multiplexed RT-PCR microsphere array assay for the detection of foot-and-mouth disease virus and look-alike disease viruses
Energy Technology Data Exchange (ETDEWEB)
Hindson, B J; Reid, S M; Baker, B R; Ebert, K; Ferris, N P; Bentley Tammero, L F; Lenhoff, R J; Naraghi-Arani, P; Vitalis, E A; Slezak, T R; Hullinger, P J; King, D P
2007-07-26
A high-throughput multiplexed assay was developed for the differential laboratory diagnosis of foot-and-mouth disease virus (FMDV) from viruses which cause clinically similar diseases of livestock. This assay simultaneously screens for five RNA and two DNA viruses using multiplexed reverse transcription PCR (mRT-PCR) amplification coupled with a microsphere hybridization array and flow-cytometric detection. Two of the seventeen primer-probe sets included in this multiplex assay were adopted from previously characterized real-time RT-PCR (rRT-PCR) assays for FMDV. The diagnostic accuracy of the mRT-PCR was evaluated using 287 field samples, including 248 (true positive n= 213, true negative n=34) from suspect cases of foot-and-mouth disease collected from 65 countries between 1965 and 2006 and 39 true negative samples collected from healthy animals. The mRT-PCR assay results were compared with two singleplex rRT-PCR assays, using virus isolation with antigen-ELISA as the reference method. The diagnostic sensitivity of the mRT-PCR assay for FMDV was 93.9% [95% C.I. 89.8-96.4%], compared to 98.1% [95% C.I. 95.3-99.3%] for the two singleplex rRT-PCR assays used in combination. In addition, the assay could reliably differentiate between FMDV and other vesicular viruses such as swine vesicular disease virus and vesicular exanthema of swine virus. Interestingly, the mRT-PCR detected parapoxvirus (n=2) and bovine viral diarrhea virus (n=2) in clinical samples, demonstrating the screening potential of this mRT-PCR assay to identify viruses in FMDV-negative material not previously recognized using focused single-target rRT-PCR assays.
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Determinantes del trabajo infantil y la escolaridad: el caso del Valle del Cauca en Colombia
Directory of Open Access Journals (Sweden)
Sebastián Urueña Abadía
2009-10-01
Full Text Available Colombia, al igual que la mayoría de países latinoamericanos, consciente de las repercusiones sociales y económicas negativas del trabajo infantil, se ha comprometido en la lucha por la erradicación de este fenómeno. Prueba de ello es la ratificación por parte del gobierno colombiano en el año 2007, del Convenio 182 de la OIT sobre la Prohibición de las Peores Formas de Trabajo Infantil y la Acción Inmediata para su Eliminación. En el plano regional, como parte del Plan de Desarrollo del Departamento del Valle del Cauca 2008-2011, se está implementando actualmente una estrategia territorial de erradicación del trabajo infantil en sus 42 municipios. Considerando este panorama, este trabajo indaga cuáles son los determinantes del trabajo infantil y la escolaridad en el departamento del Valle a partir de los datos de la Encuesta Nacional de Calidad de Vida del año 2003. Para el análisis se emplea un modelo econométrico Probit Bivariado, que permite el estudio conjunto e interrelacionado de decisiones diferentes, en este caso la asistencia escolar y el trabajo.
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Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis.
Science.gov (United States)
Nguyen, Duc Quan; Eamens, Andrew L; Grof, Christopher P L
2018-01-01
Quantitative real-time polymerase chain reaction (RT-qPCR) is the key platform for the quantitative analysis of gene expression in a wide range of experimental systems and conditions. However, the accuracy and reproducibility of gene expression quantification via RT-qPCR is entirely dependent on the identification of reliable reference genes for data normalisation. Green foxtail ( Setaria viridis ) has recently been proposed as a potential experimental model for the study of C 4 photosynthesis and is closely related to many economically important crop species of the Panicoideae subfamily of grasses, including Zea mays (maize), Sorghum bicolor (sorghum) and Sacchurum officinarum (sugarcane). Setaria viridis (Accession 10) possesses a number of key traits as an experimental model, namely; (i) a small sized, sequenced and well annotated genome; (ii) short stature and generation time; (iii) prolific seed production, and; (iv) is amendable to Agrobacterium tumefaciens -mediated transformation. There is currently however, a lack of reference gene expression information for Setaria viridis ( S. viridis ). We therefore aimed to identify a cohort of suitable S. viridis reference genes for accurate and reliable normalisation of S. viridis RT-qPCR expression data. Eleven putative candidate reference genes were identified and examined across thirteen different S. viridis tissues. Of these, the geNorm and NormFinder analysis software identified SERINE / THERONINE - PROTEIN PHOSPHATASE 2A ( PP2A ), 5 '- ADENYLYLSULFATE REDUCTASE 6 ( ASPR6 ) and DUAL SPECIFICITY PHOSPHATASE ( DUSP ) as the most suitable combination of reference genes for the accurate and reliable normalisation of S. viridis RT-qPCR expression data. To demonstrate the suitability of the three selected reference genes, PP2A , ASPR6 and DUSP , were used to normalise the expression of CINNAMYL ALCOHOL DEHYDROGENASE ( CAD ) genes across the same tissues. This approach readily demonstrated the suitably of the three
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Used mixed oxide fuel reprocessing at RT-1 plant
Energy Technology Data Exchange (ETDEWEB)
Kolupaev, D.; Logunov, M.; Mashkin, A.; Bugrov, K.; Korchenkin, K. [FSUE PA ' Mayak' , 30, Lenins str, Ozersk, 460065 (Russian Federation); Shadrin, A.; Dvoeglazov, K. [ITCP ' PRORYV' , 2/8 Malaya Krasmoselskay str, Moscow, 107140 (Russian Federation)
2016-07-01
Reprocessing of the mixed uranium-plutonium spent nuclear fuel of the BN-600 reactor was performed at the RT-1 plant twice, in 2012 and 2014. In total, 8 fuel assemblies with a burn-up from 73 to 89 GW day/t and the cooling time from 17 to 21 years were reprocessed. The reprocessing included the stages of dissolution, clarification, extraction separation of U and Pu with purification from the fission products, refining of uranium and plutonium at the relevant refining cycles. Dissolution of the fuel composition of MOX used nuclear fuel (UNF) in nitric acid solutions in the presence of fluoride ion has occurred with the full transfer of actinides into solution. Due to the high content of Pu extraction separation of U and Pu was carried out on a nuclear-safe equipment designed for the reprocessing of highly enriched U spent nuclear fuel and Pu refining. Technological processes of extraction, separation and refining of actinides proceeded without deviations from the normal mode. The output flow of the extraction outlets in their compositions corresponded to the regulatory norms and remained at the level of the compositions of the streams resulting from the reprocessing of fuel types typical for the RT-1 plant. No increased losses of Pu into waste have been registered during the reprocessing of BN-600 MOX UNF an compare with VVER-440 uranium UNF reprocessing. (authors)
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NASA SPoRT Initialization Datasets for Local Model Runs in the Environmental Modeling System
Science.gov (United States)
Case, Jonathan L.; LaFontaine, Frank J.; Molthan, Andrew L.; Carcione, Brian; Wood, Lance; Maloney, Joseph; Estupinan, Jeral; Medlin, Jeffrey M.; Blottman, Peter; Rozumalski, Robert A.
2011-01-01
The NASA Short-term Prediction Research and Transition (SPoRT) Center has developed several products for its National Weather Service (NWS) partners that can be used to initialize local model runs within the Weather Research and Forecasting (WRF) Environmental Modeling System (EMS). These real-time datasets consist of surface-based information updated at least once per day, and produced in a composite or gridded product that is easily incorporated into the WRF EMS. The primary goal for making these NASA datasets available to the WRF EMS community is to provide timely and high-quality information at a spatial resolution comparable to that used in the local model configurations (i.e., convection-allowing scales). The current suite of SPoRT products supported in the WRF EMS include a Sea Surface Temperature (SST) composite, a Great Lakes sea-ice extent, a Greenness Vegetation Fraction (GVF) composite, and Land Information System (LIS) gridded output. The SPoRT SST composite is a blend of primarily the Moderate Resolution Imaging Spectroradiometer (MODIS) infrared and Advanced Microwave Scanning Radiometer for Earth Observing System data for non-precipitation coverage over the oceans at 2-km resolution. The composite includes a special lake surface temperature analysis over the Great Lakes using contributions from the Remote Sensing Systems temperature data. The Great Lakes Environmental Research Laboratory Ice Percentage product is used to create a sea-ice mask in the SPoRT SST composite. The sea-ice mask is produced daily (in-season) at 1.8-km resolution and identifies ice percentage from 0 100% in 10% increments, with values above 90% flagged as ice.
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Future of the reprocessing business at the RT-1 plant
International Nuclear Information System (INIS)
Bukharin, O.
1995-01-01
Economic viability of reprocessing operations at the RT-1 plant is provided by the contracts with nuclear utilities from Finland and Hungary. Finland will stop sending fuel to Mayak for reprocessing after 1996. Hungary will be capable to resolve the problem of spent fuel domestically some time in the future. This increases vulnerability of the reprocessing business at Mayak to future political uncertainties. (author)
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RT-qPCR work-flow for single-cell data analysis
Czech Academy of Sciences Publication Activity Database
Stahlberg, A.; Rusňáková, Vendula; Forootan, A.; Anděrová, Miroslava; Kubista, Mikael
2013-01-01
Roč. 59, č. 1 (2013), s. 80-88 ISSN 1046-2023 R&D Projects: GA MŠk(CZ) ME10052; GA ČR GAP303/10/1338 Institutional research plan: CEZ:AV0Z50520701 Institutional support: RVO:68378041 Keywords : RT-qPCR * Single-cell biology * Single-cell data analysis Subject RIV: EB - Genetics ; Molecular Biology; FH - Neurology (UEM-P) Impact factor: 3.221, year: 2013
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Publicación y factores asociados en docentes universitarios de investigación científica de escuelas de medicina del Perú
Directory of Open Access Journals (Sweden)
Reneé Pereyra-Elías
Full Text Available Objetivos. Evaluar la frecuencia de publicación y sus factores asociados en docentes universitarios de investigación científica de escuelas de Medicina del Perú. Materiales y métodos. Estudio de corte transversal analítico. Se incluyó a todos los docentes universitarios de los cursos de investigación de las 32 escuelas de medicina del Perú en el año 2011. La búsqueda de publicaciones se realizó mediante Google Scholar, SCOPUS y Medline. Se calculó las razones de prevalencias crudas y ajustadas (RPa con intervalos de confianza al 95% mediante regresión de Poisson simple y múltiple con varianza robusta. Resultados. De los 201 docentes universitarios, 43,8% nunca ha publicado un artículo en una revista, 26,9% publicó un artículo original en una revista indizada en Medline y 16,4% lo hizo en los últimos dos años. Solo 3,0% han sido autores corresponsales en alguna revista indizada no peruana. Los factores asociados con haber publicado un artículo original en Medline durante los dos últimos años es ser menor de 40 años de edad (RPa: 2,97; IC 95%:1,21-7,32, ser profesor en una universidad donde se requiere tesis obligatoria para graduarse (RPa: 8,84; IC 95%: 2,60-30,12 y trabajar para una universidad altamente productiva (RPa: 3,24; IC 95%: 1,03-10,20. Conclusiones. La frecuencia de publicación de los docentes en investigación de las escuelas de medicina del Perú es baja. Los docentes universitarios jóvenes y los que trabajan para universidades científicamente productivas presentaron más probabilidades de publicar en una revista indizada en Medline
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La superación del modelo del ángel del hogar:
OpenAIRE
Hurtado Muñoz, Mónica
2013-01-01
La Tesis Doctoral titulada "La superación del modelo del "ángel del hogar". Recuperación de la escritora Leonor Canalejas y Fustegueras (1869-1945)" aborda, en primer lugar, un análisis sociológico de las circunstancias que rodearon a la mujer española en el cambio de siglo en el ámbito social y educativo, en un intento de esclarecer las características propias del contexto en el que la mujer accede a la intelectualidad en general y a la literatura en particular. Esta parte concluye que la cr...
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