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Sample records for darlingi root em

  1. Wing geometry of Anopheles darlingi Root (Diptera: Culicidae) in five major Brazilian ecoregions.

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    Motoki, Maysa Tiemi; Suesdek, Lincoln; Bergo, Eduardo Sterlino; Sallum, Maria Anice Mureb

    2012-08-01

    We undertook geometric morphometric analysis of wing venation to assess this character's ability to distinguish Anopheles darlingi Root populations and to test the hypothesis that populations from coastal areas of the Brazilian Atlantic Forest differ from those of the interior Atlantic Forest, Cerrado, and the regions South and North of the Amazon River. Results suggest that populations from the coastal and interior Atlantic Forest are more similar to each other than to any of the other regional populations. Notably, the Cerrado population was more similar to that from north of the Amazon River than to that collected of south of the River, thus showing no correlation with geographical distances. We hypothesize that environmental and ecological factors may affect wing evolution in An. darlingi. Although it is premature to associate environmental and ecological determinants with wing features and evolution of the species, investigations on this field are promising. Copyright © 2012 Elsevier B.V. All rights reserved.

  2. Brazilian Anopheles darlingi Root (Diptera: Culicidae) Clusters by Major Biogeographical Region.

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    Emerson, Kevin J; Conn, Jan E; Bergo, Eduardo S; Randel, Melissa A; Sallum, Maria Anice M

    2015-01-01

    The major drivers of the extensive biodiversity of the Neotropics are proposed to be geological and tectonic events together with Pliocene and Pleistocene environmental and climatic change. Geographical barriers represented by the rivers Amazonas/Solimões, the Andes and the coastal mountain ranges in eastern Brazil have been hypothesized to lead to diversification within the primary malaria vector, Anopheles (Nyssorhynchus) darlingi Root, which primarily inhabits rainforest. To test this biogeographical hypothesis, we analyzed 786 single nucleotide polymorphisms (SNPs) in 12 populations of An. darlingi from across the complex Brazilian landscape. Both model-based (STRUCTURE) and non-model-based (Principal Components and Discriminant Analysis) analysis of population structure detected three major genetic clusters that correspond with newly described Neotropical biogeographical regions: 1) Atlantic Forest province (= southeast population); 2) Parana Forest province (= West Atlantic forest population, with one Chacoan population - SP); and 3) Brazilian dominion population (= Amazonian population with one Chacoan population - TO). Significant levels of pairwise genetic divergences were found among the three clusters, allele sharing among clusters was negligible, and geographical distance did not contribute to differentiation. We infer that the Atlantic forest coastal mountain range limited dispersal between the Atlantic Forest province and the Parana Forest province populations, and that the large, diagonal open vegetation region of the Chacoan dominion dramatically reduced dispersal between the Parana and Brazilian dominion populations. We hypothesize that the three genetic clusters may represent three putative species.

  3. Heterochromatin variation in chromosomes of Anopheles (Nyssorhynchus darlingi Root and A.(N. nuneztovari Gabaldón (Diptera: Culicidae

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    Míriam Silva Rafael

    2000-03-01

    Full Text Available C-banding was used to study the variations in heterochromatic block markings in chromosomes of Anopheles darlingi and A. nuneztovari from Manaus, State of Amazonas, and Macapá, State of Amapá, Brazil. Both species had two differently shaped X chromosomes and a Y chromosome that was entirely heterochromatic. The X1 chromosome of A. darlingi had markings that extended 1/3 of the total length whereas in the X2 chromosome the markings were located around the centromeric region. The markings on autosomal chromosomes were concentrated in the centromeric region in both species, with a heterochromatic block in one arm of chromosome II of A. darlingi. A. nuneztovari had three heterochromatic blocks in chromosome X1 (longer and two blocks in X2 (shorter. X2X2 females were not detected in either species. The X1 and X2 chromosomes of males were found in A. darlingi, whereas in A. nuneztovari only the X1 chromosome was detected. Only intraspecific variation was found in heterochromatic block markings in the sex chromosomes and autosomes in the two populations of both species at each location.Pela técnica do bandamento C detectou-se variação de marcação dos blocos heterocromáticos dos cromossomos de A. darlingi e A. nuneztovari de Manaus, Amazonas, e de Macapá, Amapá, Brasil. Os cromossomos sexuais de ambas as espécies mostraram duas formas de cromossomos X e o Y foi totalmente heterocromático. No cromossomo X1 de A. darlingi a marcação atingiu 1/3 e no cromossomo X2 foi apenas na região centromérica. Nos autossomos de ambas as espécies as marcações foram constantes nas regiões centroméricas, e o cromossomo II de A. darlingi mostrou um bloco heterocromático em um dos braços. A. nuneztovari mostrou polimorfismo de tamanho para o cromossomo X, tendo o X maior (X1 três blocos e o menor (X2 dois blocos heterocromáticos. Fêmeas homozigotas (X2X2 não foram detectadas nas duas localidades. Em machos de A. darlingi foram encontrados os

  4. First Record of Anopheles darlingi Root (Diptera, Culicidae in the Volta Grande Environmental Reserve, Conceição das Alagoas Municipality, Minas Gerais, Brazil

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    Rafael Martins

    2015-04-01

    Resumo. Três espécimes de Anopheles darlingi Root foram encontrados na reserva ambiental Volta Grande, na área de influência da barragem do Rio Grande construída pela usina hidrelétrica de Volta Grande, em Conceição das Alagoas, Minas Gerais, Brasil. O mosquito An. darlingi é um dos principais vetores da malária humana no Brasil, devido à sua preferência alimentar por sangue humano, um fator que é acentuado pelo comportamento endofílico da espécie. O presente relato poderá ser útil à vigilância entomológica local para monitoramento dos impactos gerados pela formação do reservatório hidrelétrico, uma vez que a presença deste vetor neste tipo de ambiente indica um risco potencial de transmissão da malária.

  5. Ecologia de Anopheles (Nyssorhynchus darlingi Root em área de implantação de empreendimento hidrelétrico, na divisa dos Estados do Mato Grosso do Sul e São Paulo Ecology of Anopheles (Nyssorhynchus darlingi Root in the installation area of a hydroelectric scheme on the border between the States of Mato Grosso do Sul and São Paulo

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    Almério de Castro Gomes

    2010-06-01

    Full Text Available INTRODUÇÃO: Hidrelétricas alteram o fluxo das águas e provocam impactos sobre a composição de mosquitos, justificando-se essa pesquisa. O objetivo da pesquisa foi estudar anofelinos de área sob a influência de um novo lago e avaliar a vulnerabilidade relativa à malária. MÉTODOS: Foram feitas coletas de Anopheles nas margens da Represa Porto Primavera, durante as fases do alagamento até sua cota máxima. Utilizaram-se as técnicas: atrativa humana, de armadilha de Shannon e concha entomológica. Os indicadores Riqueza e Diversidade foram utilizados para medir o impacto. A análise das distribuições temporais foi realizada pelo teste Mann-Whitney, considerando localidade, cota e método de captura como variáveis independentes (α=0,05. RESULTADOS: A densidade de Anopheles darlingi oscilou entre as localidades A, B e C, sendo que os maiores picos foram para B e C. Com a estabilidade do lago, no último nível, evidenciou-se a tendência de redução da densidade de Anopheles darlingi. CONCLUSÕES: Sugere-se que o risco de autoctonia de malária nas proximidades do lago permanece inalterado, ficando o alerta para esporádicas infecções humanas.IINTRODUCTION: Hydroelectric schemes modify the water flow and cause an impact on mosquito composition, thus justifying investigations. The aim of this study was to study anophelines in the area under the influence of a new lake and to evaluate their relative vulnerability to malaria. METHODS: Anopheles specimens were collected from the edges of the Porto Primavera reservoir, during the phases of reservoir filling until its maximum level was reached. The techniques used were attraction to humans, Shannon traps and entomological scoops. The richness and diversity indexes were used to measure the impact. The temporal distribution analysis was performed using the Mann-Whitney test, taking the site, level and capture method as independent variables (α = 0.05. RESULTS: The density of Anopheles

  6. Morphometric comparisons of the scanning electron micrographs of the eggs of Anopheles (Nyssorhynchus) darlingi Root (Diptera: Culicidae).

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    Almeida, Fabio; Suesdek, Lincoln; Motoki, Maysa T; Bergo, Eduardo S; Sallum, Maria Anice M

    2014-11-01

    Anopheles darlingi Root is the principal vector of Plasmodium in Brazil, but its biological variability is not well known. Morphometric analyses of scanning electron microscopy images of the eggs of An. darlingi were conducted using individuals collected in nine states of Brazil (Acre, Amapá, Espírito Santo, Pará, Paraná, Rio de Janeiro, Rondônia, São Paulo, and Tocantins). Ten attributes of the eggs (seven continuous variables and three discrete variables) were respectively measured or counted and analyzed to determine if populations from different geographical regions or biomes could be distinguished. Univariate analysis showed that the eggs from Espírito Santo were the narrowest whereas representatives from Tocantins populations had the smallest floats. Results of multivariate analyses of continuous variables showed that the first principal component (PC1), mainly represented by all four float attributes, helped to differentiate populations. The second principal component (PC2) comprised roughly the length and width of the egg. PC1 of discrete variables corresponded to the number of ribs on the float whereas PC2 was approximately equivalent to the number of discs on the micropyle. Based on those variables (continuous and discrete separately), multivariate discriminant analysis indicated that eggs from individuals collected in Tocantins were distinct from the other populations. Among sampled localities, the one from the state of Tocantins was situated within the Cerrado biome whereas the locality from São Paulo state was at the border of Cerrado, within a transition zone of the Atlantic Forest biome. Generally, the climate in the Cerrado biome was more arid than in areas of the Amazon and Atlantic Forest biomes, and the temperature had the highest range. Coincidentally, based on morphometric data, cluster analysis distinguished the population from Cerrado, Tocantins from all other populations. Results of multiple regression analysis of the variables

  7. Microgeographic Genetic Variation of the Malaria Vector Anopheles darlingi Root (Diptera: Culicidae) from Córdoba and Antioquia, Colombia

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    Gutiérrez, Lina A.; Gómez, Giovan F.; González, John J.; Castro, Martha I.; Luckhart, Shirley; Conn, Jan E.; Correa, Margarita M.

    2010-01-01

    Anopheles darlingi is an important vector of Plasmodium spp. in several malaria-endemic regions of Colombia. This study was conducted to test genetic variation of An. darlingi at a microgeographic scale (approximately 100 km) from localities in Córdoba and Antioquia states, in western Colombia, to better understand the potential contribution of population genetics to local malaria control programs. Microsatellite loci: nuclear white and cytochrome oxidase subunit I (COI) gene sequences were analyzed. The northern white gene lineage was exclusively distributed in Córdoba and Antioquia and shared COI haplotypes were highly represented in mosquitoes from both states. COI analyses showed these An. darlingi are genetically closer to Central American populations than southern South American populations. Overall microsatellites and COI analysis showed low to moderate genetic differentiation among populations in northwestern Colombia. Given the existence of high gene flow between An. darlingi populations of Córdoba and Antioquia, integrated vector control strategies could be developed in this region of Colombia. PMID:20595475

  8. Population structure of the malaria vector <em>Anopheles darlingi> in a malaria-endemic region of Eastern Amazonian Brazil

    DEFF Research Database (Denmark)

    Conn, Jan E.; Vineis, Joseph H.; Bollback, Jonathan Paul

    2006-01-01

    of insecticides, but since the mid-1990s there has been a shift to patient treatment and focal insecticide fogging. Anopheles darlingi was believed to have been significantly reduced in a gold-mining community, Peixoto de Azevedo (in Mato Grosso State), in the early 1990s by insecticide use during a severe...... malaria epidemic. In contrast, although An. darlingi was eradicated from some districts of the city of Belem (the capital of Para State) in 1968 to reduce malaria, populations around the water protection area in the eastern district were treated only briefly. To investigate the population structure of An...

  9. [Susceptibility to insecticides of Anopheles darlingi Root 1840, in two locations of the departments of Santander and Caquetá, Colombia].

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    Santacoloma, Liliana; Tibaduiza, Tania; Gutiérrrez, Marcela; Brochero, Helena

    2012-03-01

    Physiological resistance to insecticides used in public health is the main factor to define strategies for malaria vector control. To determine the physiological status of insecticide susceptibility of natural populations of An. darlingi from two localities in the Santander and Caquetá departments. Wild adult Anopheles darlingi females were collected and bioassays using technical grade insecticides were performed following the methods recommended both by the World Health Organization (WHO, 1981) and the Centers for Diseases Control and Prevention (CDC, 1998). The natural populations of An. darlingi from the villages of Gerumano, Solano, Caqueta and Las Margaritas, San Vicente de Chucurí, Santander, showed susceptibility to the pyrethroids lambdacyhalothrin and deltamethrin, to the organochlorate DDT and to the organophosphate fenitrothion with 100% mortality rates in all of the CDC tests and between 95 and 100% in the tests performed following the WHO methods. For the carbamate propoxur the 88% mortality rate obtained in the village of Gerumano following the methods recommended by WHO coincides with the surveillance methods established for surveillance of this molecule. Chemical products whose active ingredients are the molecules tested are effective for control of An. darlingi in the study sites.

  10. Comportamiento estacional del Anopheles (nyssorhynchus darlingi root 1926 en localidades de Loreto y Madre de Dios, Perú 1999- 2000

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    Walter León C

    2003-03-01

    Full Text Available Objetivos: Determinar el comportamiento estacional del Anopheles darlingi en las localidades de Santa Clara (Loreto y Villa Luz (Madre de Dios. Materiales y métodos: En las localidades de Santa Clara y Villa Luz, entre agosto de 1999 y junio de 2000 se realizó mensualmente la inspección de criaderos, colecta de larvas de Anopheles darlingi por el método del cucharón y colecta de mosquitos adultos por el método cebo humano (intradomicilio y peri domicilió, trampa Shannon y refugio animal (extradomicilio. Se calcularon los indicadores: criadero positivo y densidad larvaria por cucharonada, índice de picadura hombre noche (IPHN, índice de picadura hombre hora (IPHH, índice esporozoítico y tasa de paridad. Resultados: El IPHN en ambas localidades se incrementó en la estación lluviosa con los valores más altos en mayo (Santa Clara y febrero (Villa Luz. En Santa Clara, el comportamiento de la picadura del Anopheles darlingi de agosto a diciembre de 1999, fue unimodal presentándose el pico de IPHH entre las 19.00 y 21.00 horas; sin embargo, de marzo a junio de 2000, el comportamiento fue bimodal con dos picos del IPHH: entre las 19.00 y 22.00 horas, y entre las 2.00 y 4.00 horas. En Villa Luz, el comportamiento de la picadura, de agosto a junio de 1999, se mantuvo unimodal, con el pico de IPHH entre las 21.00 y 24.00 horas. Las especies inmaduras de Anopheles darlingi representaron menos del 20% de las larvas encontradas en los criaderos permanentes. Conclusiones: El Anopheles darlingi presenta mayor densidad poblacional en meses de estación lluviosa, con comportamientos de picadura distintos según localidad y estación. Los criaderos evaluados no serían criaderos tan importantes de esta especie.

  11. Infection of Laboratory-Colonized Anopheles darlingi Mosquitoes by Plasmodium vivax

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    Moreno, Marta; Tong, Carlos; Guzmán, Mitchel; Chuquiyauri, Raul; Llanos-Cuentas, Alejandro; Rodriguez, Hugo; Gamboa, Dionicia; Meister, Stephan; Winzeler, Elizabeth A.; Maguina, Paula; Conn, Jan E.; Vinetz, Joseph M.

    2014-01-01

    Anopheles darlingi Root is the most important malaria vector in the Amazonia region of South America. However, continuous propagation of An. darlingi in the laboratory has been elusive, limiting entomological, genetic/genomic, and vector–pathogen interaction studies of this mosquito species. Here, we report the establishment of an An. darlingi colony derived from wild-caught mosquitoes obtained in the northeastern Peruvian Amazon region of Iquitos in the Loreto Department. We show that the numbers of eggs, larvae, pupae, and adults continue to rise at least to the F6 generation. Comparison of feeding Plasmodium vivax ex vivo of F4 and F5 to F1 generation mosquitoes showed the comparable presence of oocysts and sporozoites, with numbers that corresponded to blood-stage asexual parasitemia and gametocytemia, confirming P. vivax vectorial capacity in the colonized mosquitoes. These results provide new avenues for research on An. darlingi biology and study of An. darlingi–Plasmodium interactions. PMID:24534811

  12. Variabilidade genética em populações de Anopheles darlingi (Diptera: Culicidae e relação ao comportamento da atividade de picar, analisada por RAPD Genetic variability in populations of Anopheles darlingi (Diptera: Culicidae and relationship to biting activity behavior as analyzed by RAPD

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    Ana Paula Barbosa da Silva

    2010-09-01

    Full Text Available Duas populações naturais de Anopheles darlingi foram analisadas quanto aos padrões de variabilidade genética relacionados ao comportamento hematofágico, cujas coletas foram realizadas no intra, peri e extradomicíio, em dois municípios do Estado do Amazonas: Coari e Manaus. Os resultados evidenciaram amplo número de fragmentos polimórficos, bem como elevada variabilidade genética nessas populações. Na população de Coari, a porcentagem de locos polimórficos (P e heterozigosidade (He variou de 77,63% - 84,86% e 0,2851 0,3069, respectivamente, sendo a maior variabilidade genética detectada nas subpopulações do intradomicílio, e a menor nas do peridomicílio. A população de Manaus mostrou variabilidade genética similar a de Coari (P= 75% - 78,94% e He= 0,2732 0,2741, onde também foi detectada maior variabilidade genética no intradomicílio. Os dados de qui-quadrado (x²= 695,89; GL= 304; P Two natural populations of Anopheles darlingi, collected in the intra, peri and extra domicile of two townships in the State of Amazonas, Coari and Manaus, were assayed as to their hematophagic behaviour-related genetic variability patterns. Findings revealed a large number of polymorphic fragments as well as high genetic variability in these populations. Polymorphic loci rate (P and heterozygosity (He in the Coari population varied between 77.63% - 84.86% and 0.2851 0.3069, respectively, with the highest and lowest genetic variability being detected in the intra- and peri-domicile sub-populations, respectively. The Manaus population showed genetic variability and heterozygosity similar to those in Coari (P= 75% - 78.94% and He= 0.2732 0.2741, where higher genetic variability was detected in the intra-domicile as well. Chi-square data (x²= 695.89; GL= 304; P < 0.001 and F ST (F ST= 0.0775 ± 0.0072 were significant, indicating micro-geographic structuring resulting from some decreased gene flow. These findings point out the selective

  13. Anopheles darlingi and Anopheles marajoara (Diptera: Culicidae) susceptibility to pyrethroids in an endemic area of the Brazilian Amazon

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    Galardo, Allan Kardec Ribeiro; Póvoa, Marinete Marins; Sucupira, Izis Monica Carvalho; Galardo, Clícia Denis; Santos, Roseli La Corte dos

    2015-01-01

    Abstract: INTRODUCTION: This study aimed to evaluate the susceptibility of Anopheles darlingi Root (1926) and Anopheles marajoara Galvão & Damasceno (1942) to pyrethroids used by the National Malaria Control Program in Brazil. METHODS: Mosquitoes from Amapá, Brazilian Amazon, were assessed for resistance to cypermethrin, deltamethrin, and alpha-cypermethrin. Insecticide-impregnated bottles were used as suggested by the CDC/Atlanta. RESULTS: Diagnostic dose for Anopheles darlingi was 12.5µg...

  14. Effects of flooding of the River Paraná on the temporal activity of Anopheles (Nyssorhynchus darlingi Root (Diptera: Culicidae, at the border state of Mato Grosso do Sul and São Paulo, Brazil Efeitos do alagamento do Rio Paraná na atividade horária de Anopheles (Nyssorhynchus darlingi Root (Diptera: Culicidae, na divisa dos estados do Mato Grosso do Sul e São Paulo, Brasil

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    Almério de Castro Gomes

    2010-10-01

    primeira, executada durante 24 horas, forneceu o ritmo nictimeral e a segunda, com duração de 15 horas, para acompanhar os anofelinos durante as duas inundações. RESULTADOS: O padrão bimodal de Anopheles darlingi definido antes dessas inundações foi alterado ao longo das intervenções ambientais. O mesmo efeito repercutiu nas populações de An albitarsis s.l., An triannulatus e An galvaoi. A atividade pré-crepuscular foi a menos afetada pelas alterações ambientais. CONCLUSÕES: As barragens provocam mudanças no padrão de atividade horária dos anofelinos permitindo classificar a área em situação ecologicamente estável e instável. Diferenças observadas no comportamento dos anofelinos pelos métodos de captura mostram a influência da uni e múltipla atratividade dentro das populações estudadas.

  15. Anopheles darlingi and Anopheles marajoara (Diptera: Culicidae susceptibility to pyrethroids in an endemic area of the Brazilian Amazon

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    Allan Kardec Ribeiro Galardo

    2015-12-01

    Full Text Available Abstract: INTRODUCTION: This study aimed to evaluate the susceptibility of Anopheles darlingi Root (1926 and Anopheles marajoara Galvão & Damasceno (1942 to pyrethroids used by the National Malaria Control Program in Brazil. METHODS: Mosquitoes from Amapá, Brazilian Amazon, were assessed for resistance to cypermethrin, deltamethrin, and alpha-cypermethrin. Insecticide-impregnated bottles were used as suggested by the CDC/Atlanta. RESULTS: Diagnostic dose for Anopheles darlingi was 12.5µg/bottle during 30 min of exposure. Concentrations for Anopheles marajoara were 20µg/bottle of cypermethrin and deltamethrin and 12.5µg/bottle of alpha-cypermethrin. CONCLUSIONS : No resistance was recorded for Anopheles darlingi , but Anopheles marajoara requires attention.

  16. Anopheles darlingi and Anopheles marajoara (Diptera: Culicidae) susceptibility to pyrethroids in an endemic area of the Brazilian Amazon.

    Science.gov (United States)

    Galardo, Allan Kardec Ribeiro; Póvoa, Marinete Marins; Sucupira, Izis Monica Carvalho; Galardo, Clícia Denis; Santos, Roseli La Corte Dos

    2015-01-01

    This study aimed to evaluate the susceptibility of Anopheles darlingi Root (1926) and Anopheles marajoara Galvão & Damasceno (1942) to pyrethroids used by the National Malaria Control Program in Brazil. Mosquitoes from Amapá, Brazilian Amazon, were assessed for resistance to cypermethrin, deltamethrin, and alpha-cypermethrin. Insecticide-impregnated bottles were used as suggested by the CDC/Atlanta. Diagnostic dose for Anopheles darlingi was 12.5µg/bottle during 30 min of exposure. Concentrations for Anopheles marajoara were 20µg/bottle of cypermethrin and deltamethrin and 12.5µg/bottle of alpha-cypermethrin. No resistance was recorded for Anopheles darlingi , but Anopheles marajoara requires attention.

  17. Alkaloids Isolated from the Lateral Root of <em>Aconitum carmichaeliiem>

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    Qin-Mei Zhou

    2012-08-01

    Full Text Available Two new alkaloids, aconicarmine (1 and aconicaramide (5, were isolated from the EtOH extract of the lateral roots of <em>Aconitum carmichaeliiem>, together with five known compounds: fuziline (2, neoline (3, <em>N>-ethylhokbusine B (4, 5-hydroxymethylpyrrole-2-carbaldehyde (6, and oleracein E (7. Their structures were elucidated by physical and NMR analysis. Pyrrole alkaloids were isolated from <em>A. carmichaeliiem> for the first time. In the <em>in vitroem> assays, compounds 2 and 3 showed activity against pentobarbital sodium-induced cardiomyocytes damage by obviously recovering beating rhythm and increasing the cell viability, while compounds 5 and 7 showed moderate antibacterial activity.

  18. Infection of <em>Lotus japonicusem> Roots by<em> Mesorhizobium lotiem>

    DEFF Research Database (Denmark)

    Markmann, Katharina; Radutoiu, Elena Simona; Stougaard, Jens

    2012-01-01

    Like the two important crop legumes soybean and common bean, the model legume Lotus japonicus develops determinate root nodules. L. japonicus is normally infected through root hair infection threads in a process closely synchronised with the progressing primordial cell divisions and organ...

  19. Two New Aryltetralin Lignans from the Roots of <em>Dolomiaea soulieiem>

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    Sen Zhang

    2012-05-01

    Full Text Available Two new aryltetralin-type lignans, dolomiaeasin A (1 and dolomiaeasin B (2, were isolated from the roots of <em>Dolomiaea soulieiem>. Their structures were elucidated by means of various spectroscopic analyses. The cytotoxicities of 1 and 2 were tested by the MTT method, and both compounds showed no significant cytotoxic activities against the A549 and A2780 human cancer cell lines. This is the first time that aryltetralin-type lignans were isolated from the genus <em>Dolomiaea>.

  20. Multiple blood meals in Anopheles darlingi (Diptera: Culicidae).

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    de Oliveira, Caroline Dantas; Tadei, Wanderli Pedro; Abdalla, Fábio Camargo; Paolucci Pimenta, Paulo Filemon; Marinotti, Osvaldo

    2012-12-01

    Anopheles darlingi is an important vector of human malaria in the Amazon. Adult females of this mosquito species require a blood meal to develop eggs, preferring humans to other blood sources. Although gonotrophic concordance has been described as the norm for An. darlingi, here we report An. darlingi female mosquitoes taking two or more blood meals within their first gonotrophic cycle. Only half of field-captured adult females fed one blood meal developed follicles to Christophers' stage V. This outcome is dependent on larval nutrition, as 88% of laboratory-raised well-nourished females completed the first gonotrophic cycle with only one blood meal, while less nourished females needed additional blood meals. Half of the field-captured blood-seeking An. darlingi females had follicles in intermediate (IIIa and IIIb) and final (V) stages of the gonotrophic cycle, supporting the conclusion that An. darlingi blood feed more than once during a gonotrophic cycle. Additionally, we observed females attempting to blood feed a second time during the same day. Additional studies of An. darlingi biting behavior are necessary to accurately estimate Plasmodium sp. entomologic inoculation rates throughout the An. darlingi vast geographical distribution. © 2012 The Society for Vector Ecology.

  1. The Genome of Anopheles darlingi, the main neotropical malaria vector

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    Marinotti, Osvaldo; Cerqueira, Gustavo C.; de Almeida, Luiz Gonzaga Paula; Ferro, Maria Inês Tiraboschi; Loreto, Elgion Lucio da Silva; Zaha, Arnaldo; Teixeira, Santuza M. R.; Wespiser, Adam R.; Almeida e Silva, Alexandre; Schlindwein, Aline Daiane; Pacheco, Ana Carolina Landim; da Silva, Artur Luiz da Costa; Graveley, Brenton R.; Walenz, Brian P.; Lima, Bruna de Araujo; Ribeiro, Carlos Alexandre Gomes; Nunes-Silva, Carlos Gustavo; de Carvalho, Carlos Roberto; Soares, Célia Maria de Almeida; de Menezes, Claudia Beatriz Afonso; Matiolli, Cleverson; Caffrey, Daniel; Araújo, Demetrius Antonio M.; de Oliveira, Diana Magalhães; Golenbock, Douglas; Grisard, Edmundo Carlos; Fantinatti-Garboggini, Fabiana; de Carvalho, Fabíola Marques; Barcellos, Fernando Gomes; Prosdocimi, Francisco; May, Gemma; de Azevedo Junior, Gilson Martins; Guimarães, Giselle Moura; Goldman, Gustavo Henrique; Padilha, Itácio Q. M.; Batista, Jacqueline da Silva; Ferro, Jesus Aparecido; Ribeiro, José M. C.; Fietto, Juliana Lopes Rangel; Dabbas, Karina Maia; Cerdeira, Louise; Agnez-Lima, Lucymara Fassarella; Brocchi, Marcelo; de Carvalho, Marcos Oliveira; Teixeira, Marcus de Melo; Diniz Maia, Maria de Mascena; Goldman, Maria Helena S.; Cruz Schneider, Maria Paula; Felipe, Maria Sueli Soares; Hungria, Mariangela; Nicolás, Marisa Fabiana; Pereira, Maristela; Montes, Martín Alejandro; Cantão, Maurício E.; Vincentz, Michel; Rafael, Miriam Silva; Silverman, Neal; Stoco, Patrícia Hermes; Souza, Rangel Celso; Vicentini, Renato; Gazzinelli, Ricardo Tostes; Neves, Rogério de Oliveira; Silva, Rosane; Astolfi-Filho, Spartaco; Maciel, Talles Eduardo Ferreira; Ürményi, Turán P.; Tadei, Wanderli Pedro; Camargo, Erney Plessmann; de Vasconcelos, Ana Tereza Ribeiro

    2013-01-01

    Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vector–human and vector–parasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index.php/anopheles-darlingi. PMID:23761445

  2. Evaluation of Methods for Sampling the Malaria Vector Anopheles darlingi (Diptera, Culicidae) in Suriname and the Relation With Its Biting Behavior

    NARCIS (Netherlands)

    Hiwat-van Laar, H.; Rijk, de M.; Andriessen, R.; Koenraadt, C.J.M.; Takken, W.

    2011-01-01

    The effectiveness of CO2-baited and human-baited mosquito traps for the sampling of Anopheles darlingi Root was evaluated and compared with human landing collections in Suriname. Biting preferences of this mosquito on a human host were studied and related to trapping data. Traps used were the

  3. Anopheles darlingi polytene chromosomes: revised maps including newly described inversions and evidence for population structure in Manaus.

    Science.gov (United States)

    Cornel, Anthony J; Brisco, Katherine K; Tadei, Wanderli P; Secundino, Nágila Fc; Rafael, Miriam S; Galardo, Allan Kr; Medeiros, Jansen F; Pessoa, Felipe Ac; Ríos-Velásquez, Claudia M; Lee, Yoosook; Pimenta, Paulo Fp; Lanzaro, Gregory C

    2016-05-01

    Salivary gland polytene chromosomes of 4th instar Anopheles darlingi Root were examined from multiple locations in the Brazilian Amazon. Minor modifications were made to existing polytene photomaps. These included changes to the breakpoint positions of several previously described paracentric inversions and descriptions of four new paracentric inversions, two on the right arm of chromosome 3 and two on the left arm of chromosome 3 that were found in multiple locations. A total of 18 inversions on the X (n = 1) chromosome, chromosome 2 (n = 7) and 3 (n = 11) were scored for 83 individuals from Manaus, Macapá and Porto Velho municipalities. The frequency of 2Ra inversion karyotypes in Manaus shows significant deficiency of heterozygotes (p < 0.0009). No significant linkage disequilibrium was found between inversions on chromosome 2 and 3. We hypothesize that at least two sympatric subpopulations exist within the An. darlingi population at Manaus based on inversion frequencies.

  4. Besnoitia darlingi infection in a Virginia opossum (Didelphis virginiana).

    Science.gov (United States)

    Shaw, Shannon; Grasperge, Britton; Nevarez, Javier; Reed, Scott; Long, Lauren; Rademacher, Nathalie; Sánchez-Migallón Guzmán, David

    2009-03-01

    This is a case report of natural infection with Besnoitia darlingi in a Virginia opossum (Didelphis virginiana) in Louisiana. Clinical pathologic data included a severe nonregenerative anemia, inflammatory leukogram, increased hepatocellular leakage enzymes, renal azotemia, hyperkalemia, hypoglycemia, hypoalbuminemia, and proteinuria. Tissue cysts containing bradyzoites were found in the majority of organs, especially the skin, mucous membranes, kidneys, adrenals, lungs, and heart. Images of the bradyzoites obtained by transmission electron microscopy were consistent with the previously described ultrastructure of Besnoitia darlingi. This opossum also suffered from an open phalangeal fracture and concurrent gastrointestinal parasites. Histopathologic findings included a glomerulonephritis and hepatic necrosis.

  5. The karyotype and taxonomic status of Cryptomys hottentotus darlingi

    African Journals Online (AJOL)

    The karyotype and taxonomic status of. Cryptomys hottentotus darlingi (Rodentia: Bath yergid ae). G.H, Aguilar. Department of ZOOlogy, University of Cape Town, Ronde- ..... Classification of southern African mammals. Transvaal Mus. MOflogr. 5: 1-359. NEVO. E .. CAP ANNA. E .. CORTI. M .. JARVIS. LU.M. &. HICKMAN.

  6. Bobcats (Lynx rufus) are natural definitive host of Besnoitia darlingi.

    Science.gov (United States)

    Verma, Shiv K; Cerqueira-Cézar, Camila K; Murata, Fernando H A; Lovallo, Matthew J; Rosenthal, Benjamin M; Dubey, Jitender P

    2017-12-15

    Bovine besnoitiosis, caused by Besnoitia besnoiti, is an economically important disease of cattle in many countries but its transmission remains a mystery. Wild felids are suspected to be its definitive hosts. The domestic cat (Felis catus) is known experimental definitive host for Besnoitia species of rodents. Here, we report for Besnoitia darlingi the first identification of a natural definitive host, the bobcat (Lynx rufus). Oocysts resembling Toxoplasma gondii (unsporulated; 10.9±0.8×12.1±0.2μm; n=5) were detected microscopically in the feces of two of 25 free ranging wild bobcats from Mississippi, USA. After detailed investigation, we identified these oocysts as B. darlingi and not T. gondii. The IFN-γ gene knockout (KO) mice fed oocysts from bobcats died of acute besnoitiosis and tachyzoites were found in their tissues. Oocysts were also mildly pathogenic to outbred Swiss Webster mice (SW) (Mus musculus). The SW mice fed oocysts became ill but generally survived and developed characteristic thick-walled Besnoitia tissue cysts in their tongue and heart muscles and brains. Two laboratory-raised domestic cats (Felis catus) excreted B. darlingi oocysts after ingesting murine tissues infected with bobcat-derived oocysts. The parasite was successfully cultivated in African green monkey kidney fibroblast cells (CV-1 cell line) seeded with infected murine tissue homogenate. The multilocus PCR-DNA sequencing (18S rDNA, 28S rDNA, and ITS-1) from culture-derived tachyzoites confirmed the parasite as B. darlingi. Our results suggest that bobcats may be an important link in the sylvatic cycle of Besnoitia species and bioassay or molecular tests are needed to differentiate Toxoplasma gondii-like oocysts in feces of felids, both domestic and wild cats. Published by Elsevier B.V.

  7. [Historical review of the distribution of Anopheles (Nyssorhynchus) darlingi (Diptera: Culicidae) in the Peruvian Amazon].

    Science.gov (United States)

    Fernández, Roberto; Vera, Hubert; Calderón, Guillermo

    2014-04-01

    Anopheles (Nyssorhynchus) darlingi has been reported since 1931 in border areas of the department of Loreto, mainly along the borders with Brazil and Colombia. In 1994, during an outbreak of malaria, An. darlingi was found in neighboring towns to Iquitos. At present, its distribution has expanded considerably in Loreto. This paper reviews literature available for all possible information on the distribution of mosquitoes, particularly anopheline in the Amazon region of the country, with special emphasis on An darlingi. Entomological collections were also conducted in the departments of Madre de Dios and Ucayali in order to know and verify the distribution of An. darlingi. At present, the distribution of the species is confined to localities in southeastern Peru with Bolivia border towns, in a town near the Abujao River in the department of Ucayali, and widely in the northeastern region of the Amazon basin of Loreto in Peru.

  8. Anopheles darlingi (Diptera: Culicidae Rood 1926: Morphometric variations in wings and legs of populations from Colombia

    Directory of Open Access Journals (Sweden)

    Miguel Alfonso Pacheco

    2017-03-01

    Conclusions. We registered 11 new wing spot patterns in the costal vein and the dominance of the patterns I and VI for populations of An. darlingi from Colombia. We confirmed DSIII2/TaIII2 ratio as a robust diagnostic character for the taxonomy of this species. We found differences between the size and shape of the wings of An. darlingi populations in accordance to their geographical distribution, which constitute important bionomic aspects for this malaria vector.

  9. Systematics and Population Level Analysis of Anopheles darlingi

    Directory of Open Access Journals (Sweden)

    Conn JE

    1998-01-01

    Full Text Available A new phylogenetic analysis of the Nyssorhynchus subgenus (Danoff-Burg and Conn, unpub. data using six data sets {morphological (all life stages; scanning electron micrographs of eggs; nuclear ITS2 sequences; mitochondrial COII, ND2 and ND6 sequences} revealed different topologies when each data set was analyzed separately but no heterogeneity between the data sets using the arn test. Consequently, the most accurate estimate of the phylogeny was obtained when all the data were combined. This new phylogeny supports a monophyletic Nyssorhynchus subgenus but both previously recognized sections in the subgenus (Albimanus and Argyritarsis were demonstrated to be paraphyletic relative to each other and four of the seven clades included species previously placed in both sections. One of these clades includes both Anopheles darlingi and An. albimanus, suggesting that the ability to vector malaria effectively may have originated once in this subgenus. Both a conserved (315 bp and a variable (425 bp region of the mitochondrial COI gene from 15 populations of An. darlingi from Belize, Bolivia, Brazil, French Guiana, Peru and Venezuela were used to examine the evolutionary history of this species and to test several analytical assumptions. Results demonstrated (1 parsimony analysis is equally informative compared to distance analysis using NJ; (2 clades or clusters are more strongly supported when these two regions are combined compared to either region separately; (3 evidence (in the form of remnants of older haplotype lineages for two colonization events; and (4 significant genetic divergence within the population from Peixoto de Azevedo (State of Mato Grosso, Brazil. The oldest lineage includes populations from Peixoto, Boa Vista (State of Roraima and Dourado (State of São Paulo.

  10. Prevalence and tissue distribution of Besnoitia darlingi cysts in the Virginia opossum (Didelphis virginiana) in Michigan.

    Science.gov (United States)

    Elsheikha, Hany M; Mansfield, Linda S; Fitzgerald, Scott D; Saeed, Mahdi A

    2003-08-14

    Specimens of Virginia opossums (Didelphis virginiana) in Michigan were examined over 1 year to document the presence of Besnoitia darlingi cysts. Cyst morphology, prevalence, seasonal variation, and tissue sites of isolation were studied. Histology and ultrastructural features of the detected cysts and bradyzoites were consistent with B. darlingi. In the opossums, B. darlingi had intracellular tissue cysts. Tissue cysts had a mean diameter of 560 microm and were separated from the host tissue by a thick (5-20 microm) cyst wall. Overall prevalence of B. darlingi cysts in opossums was 10.9% (15/137). Variations in the prevalence were detected during spring (3/17; 17.6%), summer (10/34; 29.4%), and fall (2/60; 3.3%). No cysts were detected in the specimens examined during winter (0/26; 0%). Numerous B. darlingi cysts were detected in ears, conjunctiva, tongue, abdominal muscles, diaphragm, stomach, heart, liver, kidney, lung, and spleen. Cysts were detected mainly in adult female opossums that were debilitated. Ear was the most frequent organ from which the cysts were reported (10/15; 66.7%) when compared individually with other body tissues (P<0.05).

  11. Intra-population plasticity of Anopheles darlingi's (Diptera, Culicidae biting activity patterns in the state of Amapá, Brazil Plasticidade intrapopulacional nos padrões de atividade hematofágica de Anopheles darlingi (Diptera: Culicidae no Estado do Amapá, Brasil

    Directory of Open Access Journals (Sweden)

    Jaco Voorham

    2002-02-01

    Full Text Available OBJECTIVE: To assess the variation in Anopheles darlingi's biting activity compared to An. marajoara in the same locality and to biting activity data from other regions. METHODS: Using human bait, eight observations of the biting activity of An. darlingi and An. marajoara were carried out during 1999 and 2000 in the municipality of São Raimundo do Pirativa, state of Amapá, Brazil. Each observation consisted of three consecutive 13-hour collections, close to full moon. There were shifts of collectors in the observation points and nocturnal periods. RESULTS: An. darlingi revealed considerable plasticity of biting activity in contrast to An. marajoara, which showed well-defined crepuscular biting peaks. No significant correlation between density and biting activity was found, but a significant correlation existed between time and proportional crepuscular activity, indicating underlying ecological processes not yet understood. Two of the four available data sets having multiple observations at one locality showed considerable plasticity of this species' biting patterns as well. CONCLUSION: Intra-population variation of biting activity can be as significant as inter-population variation. Some implications in malaria vector control and specific studies are also discussed.OBJETIVO: Examinar a variação no ciclo de atividade hematofágica de Anopheles darlingi em uma localidade, em comparação com An. marajoara na mesma localidade e com dados de atividade hematofágica de outras regiões. MÉTODOS: Durante 1999 e 2000 foram feitas oito observações da atividade de picar de An. darlingi e An. marajoara, utilizando isca humana, na localidade de São Raimundo do Pirativa, Estado de Amapá, Brasil. Cada observação era composta de três coletas consecutivas de 13 horas, situadas ao redor da ocorrência de lua cheia. Os coletores foram trocados entre os pontos de observação e os períodos noturnos. RESULTADOS: An. darlingi mostrou consider

  12. Anatomia radicular de milho em solo compactado Anatomy of maize roots in compacted soil

    Directory of Open Access Journals (Sweden)

    Anderson Cristian Bergamin

    2010-03-01

    Full Text Available O objetivo deste trabalho foi avaliar a relação entre a estrutura anatômica das raízes de milho e os atributos físicos de solo submetido a diferentes graus de compactação. O estudo foi realizado em campo, no Município de Dourados, MS, em Latossolo Vermelho distroférrico, textura muito argilosa. O delineamento experimental foi o de blocos ao acaso, com cinco repetições. O solo utilizado apresentava histórico de oito anos com plantio direto. A compactação adicional do solo foi realizada por meio de tráfego com trator, com uma (PDc1, duas (PDc2, quatro (PDc4 e seis passadas (PDc6 sucessivas, sobre toda a área da parcela. Houve aumento na razão entre o córtex e o cilindro vascular da raiz com o aumento na compactação do solo. Essa razão correlacionou-se negativamente com a macroporosidade e positivamente com a densidade do solo e com a resistência do solo à penetração. O grau de compactação afeta a anatomia radicular do milho, e a resistência do solo à penetração é o indicador físico que melhor expressa esse efeito.The objective of this work was to evaluate the relationship between maize root anatomy and physical attributes of soil subjected to levels of compaction. The experiment was carried out in field conditions, in Dourados, Mato Grosso do Sul state, Brazil, in a clayed Latossolo Vermelho (Rhodic Acrustox. A randomized complete block design was used, with five replicates. The soil was cultivated for eight years under no-tillage. The additional compaction of the soil was done by tractor traffic in one (PDc1, two (PDc2, four (PDc4 and six successive passes (PDc6, in the entire area of the experimental plots. Ratio between the cortex and vascular cylinder of the root increased with soil compaction. This ratio was negatively correlated to macroporosity, and positively to soil density and soil penetration resistance. Soil compaction level affects the anatomy of maize roots, and its penetration resistance is the best

  13. GNBP domain of Anopheles darlingi: are polymorphic inversions and gene variation related to adaptive evolution?

    Science.gov (United States)

    Bridi, L C; Rafael, M S

    2016-02-01

    Anopheles darlingi is the main malaria vector in humans in South America. In the Amazon basin, it lives along the banks of rivers and lakes, which responds to the annual hydrological cycle (dry season and rainy season). In these breeding sites, the larvae of this mosquito feed on decomposing organic and microorganisms, which can be pathogenic and trigger the activation of innate immune system pathways, such as proteins Gram-negative binding protein (GNBP). Such environmental changes affect the occurrence of polymorphic inversions especially at the heterozygote frequency, which confer adaptative advantage compared to homozygous inversions. We mapped the GNBP probe to the An. darlingi 2Rd inversion by fluorescent in situ hybridization (FISH), which was a good indicator of the GNBP immune response related to the chromosomal polymorphic inversions and adaptative evolution. To better understand the evolutionary relations and time of divergence of the GNBP of An. darlingi, we compared it with nine other mosquito GNBPs. The results of the phylogenetic analysis of the GNBP sequence between the species of mosquitoes demonstrated three clades. Clade I and II included the GNBPB5 sequence, and clade III the sequence of GNBPB1. Most of these sequences of GNBP analyzed were homologous with that of subfamily B, including that of An. gambiae (87 %), therefore suggesting that GNBP of An. darling belongs to subfamily B. This work helps us understand the role of inversion polymorphism in evolution of An. darlingi.

  14. Chymotrypsin genes in the malaria mosquitoes Anopheles aquasalis and Anopheles darlingi.

    Science.gov (United States)

    de Almeida, R W; Tovar, F J; Ferreira, I I; Leoncini, O

    2003-03-01

    Four closely related chymotrypsin genes were identified in Anopheles aquasalis and Anopheles darlingi (Anachy1, Anachy2, Andchy1 and Andchy2). The deduced amino-acid sequences were compared to other chymotrypsin sequences. These sequences were used to infer phylogenetic relationships among the different species. Genomic cloning revealed that, in contrast to An. aquasalis and A. gambiae, the chymotrypsin genomic locus in An. darlingi had a short intergenic region that accompanied the inverted position of the genes, suggesting inversion mechanisms in this species related to transposable elements. Alignments of the sequences upstream of the transcription start sites of Anachy1, Anachy2, Andchy1 and Andchy2 revealed areas with high similarity containing palindromic sequences. Northern analysis from An. aquasalis indicated that the transcription of chy 1 and 2 are induced by blood feeding.

  15. LARVICIDAL ACTIVITY OF Bacillus sphaericus 2362 AGAINST Anopheles nuneztovari, Anopheles darlingi AND Anopheles braziliensis (DIPTERA, CULICIDAE

    Directory of Open Access Journals (Sweden)

    RODRIGUES Iléa Brandão

    1999-01-01

    Full Text Available In this present study, preliminary data was obtained regarding the mortality rate of the Amazonian anophelines, Anopheles nuneztovari, Anopheles darlingi and Anopheles braziliensis when subjected to treatment with Bacillus sphaericus strain 2362, the WHO standard strain. Initially, experiments were conducted to test the mortality rate of the three species of anopheline larvae. The third larval instar of An. nuneztovari and the second and third larval instars of An. darlingi proved to be the least susceptible. In other experiments, the same three mosquito species were tested with the standard strain 2362, An. nuneztovari was the least susceptible to this insect pathogen, while An. braziliensis was the most susceptible. This latter species showed a difference in the level of LC50 concentration, when compared to the former, of 2.4, 2.5 and 1.8 in readings taken 24, 48 and 72 hours after exposure to the bacillus.

  16. Dexamethasone treatment induces susceptibility of outbred Webster mice to experimental infection with Besnoitia darlingi isolated from opossums (Didelphis virginiana).

    Science.gov (United States)

    Elsheikha, Hany M; Rosenthal, Benjamin M; Mansfield, Linda S

    2005-04-01

    The Sarcocystidae comprise a diverse, monophyletic apicomplexan parasite family, most of whose members form intracellular cysts in their intermediate hosts. The extent of pathology associated with such cyst formation can range widely. We currently lack experimental animal models for many of these infections. Here we explored dexamethasone treatment as a means to render outbred mice susceptible to Besnoitia darlingi infection and demonstrated that this approach allows viable parasites to be subsequently isolated from these mice and maintained in tissue culture. Besnoitia bradyzoites recovered from crushed cysts derived from naturally infected opossums (Didelphis virginiana) replicated and reproduced the development of besnoitiosis in mice treated with dexamethasone (0.5 mg/ml drinking water) daily for 12 days post infection (DPI). Isolates recovered from the peritoneal exudates of these mice were viable and were maintained in long-term tissue cultures. In contrast, control mice given saline without dexamethasone and challenged with similar bradyzoites remained clinically normal for up to 70 DPI. An additional group of mice challenged with the same inoculum of bradyzoites and given dexamethasone at the same concentration and treated with sulfadiazine (1 mg/ml drinking water) daily for 12 DPI also remained normal for up to 70 DPI. Severe disease developed more rapidly in dexamethasone-treated mice inoculated with culture-derived B. darlingi tachyzoites than in those inoculated with cyst-derived bradyzoites. B. darlingi tachyzoite-infected, untreated control mice developed signs of illness at 18 DPI. In contrast, mice treated with sulfadiazine showed no clinical signs up to 50 DPI. Although dexamethasone treatment was required to establish B. darlingi infection in outbred mice inoculated with opossum-derived B. darlingi bradyzoites, no such treatment was required for mice inoculated with culture-derived B. darlingi tachyzoites. Finally, sulfadiazine was highly

  17. First detection of Leishmania major DNA in Sergentomyia (Spelaeomyia) darlingi from cutaneous leishmaniasis foci in Mali.

    Science.gov (United States)

    Berdjane-Brouk, Zohra; Koné, Abdoulaye K; Djimdé, Abdoulaye A; Charrel, Rémi N; Ravel, Christophe; Delaunay, Pascal; del Giudice, Pascal; Diarra, Adama Z; Doumbo, Siala; Goita, Siaka; Thera, Mahamadou A; Depaquit, Jérôme; Marty, Pierre; Doumbo, Ogobara K; Izri, Arezki

    2012-01-01

    Leishmania major complex is the main causative agent of zoonotic cutaneous leishmaniasis (ZCL) in the Old World. Phlebotomus papatasi and Phlebotomus duboscqi are recognized vectors of L. major complex in Northern and Southern Sahara, respectively. In Mali, ZCL due to L. major is an emerging public health problem, with several cases reported from different parts of the country. The main objective of the present study was to identify the vectors of Leishmania major in the Bandiagara area, in Mali. An entomological survey was carried out in the ZCL foci of Bandiagara area. Sandflies were collected using CDC miniature light traps and sticky papers. In the field, live female Phlebotomine sandflies were identified and examined for the presence of promastigotes. The remaining sandflies were identified morphologically and tested for Leishmania by PCR in the ITS2 gene. The source of blood meal of the engorged females was determined using the cyt-b sequence. Out of the 3,259 collected sandflies, 1,324 were identified morphologically, and consisted of 20 species, of which four belonged to the genus Phlebotomus and 16 to the genus Sergentomyia. Leishmania major DNA was detected by PCR in 7 of the 446 females (1.6%), specifically 2 out of 115 Phlebotomus duboscqi specimens, and 5 from 198 Sergentomyia darlingi specimens. Human DNA was detected in one blood-fed female S. darlingi positive for L. major DNA. Our data suggest the possible involvement of P. duboscqi and potentially S. darlingi in the transmission of ZCL in Mali.

  18. Organic fertilization alters the community composition of root associated fungi in <em>Pisum sativumem>

    DEFF Research Database (Denmark)

    Yu, L.; Nicolaisen, M.; Ravnskov, S.

    2013-01-01

    of the present study was to examine the response of communities of root associated fungi in Pisum sativum to Protamylasse, an organic fertilizer used in pea production. Plants were grown in pots with field soil amended with four different levels of Protamylasse. 454 pyrosequencing was employed to examine...

  19. A Study on the Bionomics of Anopheles darlingi Root (Diptera: Culicidae) in Belize, Central America

    Science.gov (United States)

    2004-03-01

    20: 98-271. Lourenco-de-Oliveira, R., Guimaraes, A. E., Arle, M., da Silva, T.F., Castro, M.G., Motta , M.A. and Deane, L.M. 1989. Anopheline...Med. 21: 559-566. Lourenco-de-Oliveira, R., Guimaraes, A. E., Arle, M., da Silva, T.F., Castro, M.G., Motta , M.A. and Deane, L.M. 1989...Plasmodium vivax sporozoite rates from Anopheles albimanus in southern Chiapas, Mexico. J. Parasitol. 80: 489-493. Ramsey, J.M., Bown, D.N., Aron

  20. First Detection of Leishmania major DNA in Sergentomyia (Spelaeomyia) darlingi from Cutaneous Leishmaniasis Foci in Mali

    Science.gov (United States)

    Berdjane-Brouk, Zohra; Koné, Abdoulaye K.; Djimdé, Abdoulaye A.; Charrel, Rémi N.; Ravel, Christophe; Delaunay, Pascal; del Giudice, Pascal; Diarra, Adama Z.; Doumbo, Siala; Goita, Siaka; Thera, Mahamadou A.; Depaquit, Jérôme; Marty, Pierre; Doumbo, Ogobara K.; Izri, Arezki

    2012-01-01

    Background Leishmania major complex is the main causative agent of zoonotic cutaneous leishmaniasis (ZCL) in the Old World. Phlebotomus papatasi and Phlebotomus duboscqi are recognized vectors of L. major complex in Northern and Southern Sahara, respectively. In Mali, ZCL due to L. major is an emerging public health problem, with several cases reported from different parts of the country. The main objective of the present study was to identify the vectors of Leishmania major in the Bandiagara area, in Mali. Methodology/Principal Findings An entomological survey was carried out in the ZCL foci of Bandiagara area. Sandflies were collected using CDC miniature light traps and sticky papers. In the field, live female Phlebotomine sandflies were identified and examined for the presence of promastigotes. The remaining sandflies were identified morphologically and tested for Leishmania by PCR in the ITS2 gene. The source of blood meal of the engorged females was determined using the cyt-b sequence. Out of the 3,259 collected sandflies, 1,324 were identified morphologically, and consisted of 20 species, of which four belonged to the genus Phlebotomus and 16 to the genus Sergentomyia. Leishmania major DNA was detected by PCR in 7 of the 446 females (1.6%), specifically 2 out of 115 Phlebotomus duboscqi specimens, and 5 from 198 Sergentomyia darlingi specimens. Human DNA was detected in one blood-fed female S. darlingi positive for L. major DNA. Conclusion Our data suggest the possible involvement of P. duboscqi and potentially S. darlingi in the transmission of ZCL in Mali. PMID:22276095

  1. First detection of Leishmania major DNA in Sergentomyia (Spelaeomyia darlingi from cutaneous leishmaniasis foci in Mali.

    Directory of Open Access Journals (Sweden)

    Zohra Berdjane-Brouk

    Full Text Available BACKGROUND: Leishmania major complex is the main causative agent of zoonotic cutaneous leishmaniasis (ZCL in the Old World. Phlebotomus papatasi and Phlebotomus duboscqi are recognized vectors of L. major complex in Northern and Southern Sahara, respectively. In Mali, ZCL due to L. major is an emerging public health problem, with several cases reported from different parts of the country. The main objective of the present study was to identify the vectors of Leishmania major in the Bandiagara area, in Mali. METHODOLOGY/PRINCIPAL FINDINGS: An entomological survey was carried out in the ZCL foci of Bandiagara area. Sandflies were collected using CDC miniature light traps and sticky papers. In the field, live female Phlebotomine sandflies were identified and examined for the presence of promastigotes. The remaining sandflies were identified morphologically and tested for Leishmania by PCR in the ITS2 gene. The source of blood meal of the engorged females was determined using the cyt-b sequence. Out of the 3,259 collected sandflies, 1,324 were identified morphologically, and consisted of 20 species, of which four belonged to the genus Phlebotomus and 16 to the genus Sergentomyia. Leishmania major DNA was detected by PCR in 7 of the 446 females (1.6%, specifically 2 out of 115 Phlebotomus duboscqi specimens, and 5 from 198 Sergentomyia darlingi specimens. Human DNA was detected in one blood-fed female S. darlingi positive for L. major DNA. CONCLUSION: Our data suggest the possible involvement of P. duboscqi and potentially S. darlingi in the transmission of ZCL in Mali.

  2. Population dynamics, structure and behavior of Anopheles darlingi in a rural settlement in the Amazon rainforest of Acre, Brazil.

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    Moutinho, Paulo Rufalco; Gil, Luis Herman Soares; Cruz, Rafael Bastos; Ribolla, Paulo Eduardo Martins

    2011-06-24

    Anopheles darlingi is the major vector of malaria in South America, and its behavior and distribution has epidemiological importance to biomedical research. In Brazil, An. darlingi is found in the northern area of the Amazon basin, where 99.5% of the disease is reported. The study area, known as Ramal do Granada, is a rural settlement inside the Amazon basin in the state of Acre. Population variations and density have been analysed by species behaviour, and molecular analysis has been measured by ND4 mitochondrial gene sequencing. The results show higher density in collections near a recent settlement, suggesting that a high level of colonization decreases the vector presence. The biting activity showed higher activity at twilight and major numbers of mosquitos in the remaining hours of the night in months of high density. From a sample of 110 individual mosquitoes, 18 different haplotypes were presented with a diversity index of 0.895, which is higher than that found in other Anopheles studies. An. darlingi depends on forested regions for their larval and adult survival. In months with higher population density, the presence of mosquitoes persisted in the second part of the night, increasing the vector capacity of the species. Despite the intra-population variation in the transition to rainy season, the seasonal distribution of haplotypes shows no change in the structure population of An. darlingi.

  3. Location of ribosomal genes in the chromosomes of Anopheles darlingi and Anopheles nuneztovari (Diptera, Culicidae) from the Brazilian Amazon.

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    Rafael, Míriam Silva; Tadei, Wanderli Pedro; Recco-Pimentel, Shirlei Maria

    2003-07-01

    Fluorescence in situ hybridization of Anopheles darlingi and A. nuneztovari demonstrated nucleolar organizer region activity at the end of the fourth larval instar, when the nucleolar organizer regions underwent gradual condensation. The heteromorphic sex chromosomes showed intraindividual size variation in the rDNA blocks located in the pericentromeric region and this coincided with the location of constitutive heterochromatin (C-banding).

  4. Comparison of experimental hut entrance and exit behavior between Anopheles darlingi from the Cayo District, Belize, and Zungarococha, Peru.

    Science.gov (United States)

    Sachs, Paige; Diaz Rodriguez, Gloria Alicia; Briceno, Ireneo; King, Russell; Achee, Nicole L; Grieco, John P

    2013-12-01

    Anopheles darlingi is a major vector for malaria in Central and South America. Behavioral, ecological, genetic, and morphologic variability has been observed across its wide distribution. Recent studies have documented that 2 distinct genotypes exist for An. darlingi: a northern lineage (Belize, Guatemala, Colombia, Venezuela, and Panama) and a southern lineage (Amazonia and southern Brazil). In order to determine if these genotypes exhibited different behavioral traits, entrance and exit movement patterns between 2 field populations of An. darlingi that represented each genotype were evaluated using experimental huts. The Belize population exhibited bimodal entrance, with peak entry occurring between 7:00-8:00 p.m. and 5:00-6:00 a.m. and peak exiting occurring between 7:00-8:00 p.m. The Peru population exhibited unimodal entrance, with peak entry occurring between 10:00-11:00 p.m. and peak exiting occurring between 11:00-12:00 a.m. with a secondary smaller peak at 2:30 a.m. Entrance and exit behavioral patterns were significantly different between the Belize and Peru populations of An. darlingi (log-rank [Mantel-Cox] P < 0.001). Information from the present study will be used in the future to determine if there is a correlation between genotype and host-seeking behavior and can be used in the present for regional vector risk assessment.

  5. Intensive trapping of blood-fed Anopheles darlingi in Amazonian Peru reveals unexpectedly high proportions of avian blood-meals.

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    Marta Moreno

    2017-02-01

    Full Text Available Anopheles darlingi, the main malaria vector in the Neotropics, has been considered to be highly anthropophilic. However, many behavioral aspects of this species remain unknown, such as the range of blood-meal sources. Barrier screens were used to collect resting Anopheles darlingi mosquitoes from 2013 to 2015 in three riverine localities (Lupuna, Cahuide and Santa Emilia in Amazonian Peru. Overall, the Human Blood Index (HBI ranged from 0.58-0.87, with no significant variation among years or sites. Blood-meal analysis revealed that humans are the most common blood source, followed by avian hosts (Galliformes-chickens and turkeys, and human/Galliforme mixed-meals. The Forage Ratio and Selection Index both show a strong preference for Galliformes over humans in blood-fed mosquitoes. Our data show that 30% of An. darlingi fed on more than one host, including combinations of dogs, pigs, goats and rats. There appears to be a pattern of host choice in An. darlingi, with varying proportions of mosquitoes feeding only on humans, only on Galliformes and some taking mixed-meals of blood (human plus Galliforme, which was detected in the three sites in different years, indicating that there could be a structure to these populations based on blood-feeding preferences. Mosquito age, estimated in two localities, Lupuna and Cahuide, ranged widely between sites and years. This variation may reflect the range of local environmental factors that influence longevity or possibly potential changes in the ability of the mosquito to transmit the parasite. Of 6,204 resting An. darlingi tested for Plasmodium infection, 0.42% were infected with P. vivax. This study provides evidence for the first time of the usefulness of barrier screens for the collection of blood-fed resting mosquitoes to calculate the Human Blood Index (HBI and other blood-meal sources in a neotropical malaria endemic setting.

  6. Sistema radicular de cultivares de feijão em resposta à calagem Root system of common bean cultivars as response of liming

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    Laerte Marques da Silva

    2004-07-01

    Full Text Available O crescimento radicular apresenta variabilidade genética, e é afetado pelo Al tóxico e baixos teores de Ca e Mg do solo, que podem comprometer o seu desenvolvimento. O objetivo deste trabalho foi avaliar o sistema radicular de cultivares de feijão em resposta à calagem. O experimento foi realizado em vasos de 13 dm³ de solo, em casa de vegetação, até 43 dias após a emergência das plantas. O delineamento experimental utilizado foi o inteiramente casualizado, em arranjo fatorial 4x4, composto por quatro doses de calcário (0, 1,59, 3,18 e 4,48 t ha-1 e quatro cultivares de feijão (Campeão 1, Carioca, FT-Bonito e Pérola, com quatro repetições. O sistema radicular da cultivar Pérola foi mais tolerante à baixa saturação por bases do que o das demais cultivares. O máximo crescimento radicular das cultivares Campeão 1, Carioca e Pérola ocorreu em saturação por bases ao redor de 43%. Em condições de elevada acidez, o diâmetro radicular das cultivares é reduzido e há um incremento em comprimento. A calagem promove aumento do sistema radicular e da parte aérea das cultivares.Root growth is genetically controlled and the variability among cultivars and soil factors, such as Al toxicity and low Ca and Mg contents, can damage it. The objective of this study was to evaluate root growth and shoot growth of bean cultivars in response to liming. The experiment was carried out in pots with 13 dm³ of soil, in greenhouse until 43 days after the emergence of the plants. The experimental design was a completely randomized block, in a factorial scheme 4x4, with four doses of line (0, 1.59, 3.18 and 4.48 t ha-1, four bean cultivars (Campeão 1, Carioca, FT-Bonito and Pérola, and four replications. Pérola cultivar presents a root system more tolerant to low base saturation in comparison to the others. The maximum root growth occurred in base saturation values around 43% for Campeão 1, Carioca and Pérola cultivars. Under high acidity

  7. Prevalence of antibodies to Trypanosoma cruzi, Toxoplasma gondii, Encephalitozonn cuniculi, Sarcocystis neurona, Besnoitia darlingi, and Neospora caninum in North American opossum, Didelphis virginiana, from Southern Louisian

    Science.gov (United States)

    We examined the prevalence of antibodies to zoonotic protozoan parasites (Trypanosoma cruzi, Toxoplasma gondii, and Encephalitozoon cuniculi) and protozoan’s of veterinary importance (Neospora caninum, Sarcocystis neurona and Besnoitia darlingi) in a population of North American opossums (Didelphis...

  8. Sealing ability of cements in root canals prepared for intraradicular posts Capacidade seladora de cimentos em canais radiculares preparados para pinos intra-radiculares

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    Dirce Haruko Nakamura

    2006-08-01

    Full Text Available This research evaluated the sealer ability of 2 temporary filling materials (white Cimpat and IRM and 1 restorative cement (glass ionomer, in canals prepared for root posts. Sixty human palatal roots of maxillary first molars were used. They were divided into 3 groups, according to the cements used: Group I (Cimpat, Group II (IRM and Group III (glass ionomer. The roots were rendered impermeable, filled with the respective cements and soon after immersed into 0.2% Rhodamine B dye and maintained for 72 hours in an oven for 37°C. Microleakage was measured with a light microscope, cutting the roots longitudinally in buccolingual direction. The results showed that Group I presented significantly more leakage than Groups II and III, which were not significantly different from each other.Esta pesquisa avaliou a capacidade seladora de dois cimentos temporários (Cimpat branco e IRM e um cimento restaurador em canais preparados para pinos intra-radiculares. Foram usadas 60 raízes palatinas de primeiros molares superiores humanos. Elas foram divididas em 3 grupos, conforme os cimentos usados: grupo I (Cimpat, grupo II (IRM e grupo III (ionômero de vidro. As raízes foram impermeabilizadas, preenchidas com os respectivos cimentos e em seguida imersas em tintura de Rhodamina B a 0,2% e mantidas por 72 horas em uma estufa a 37ºC. A micro infiltração foi mensurada com um microscópio de luz nas raízes cortadas longitudinalmente, na direção vestíbubo-lingual. Os resultados mostraram que o grupo I apresentou, de modo significante, mais infiltração do que os grupos II e III, enquanto que entre estes não houve diferença significante.

  9. Extração de ecdisterona em raízes de ginseng brasileiro Extraction of ecdysterone from roots of Brazilian ginseng

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    Rejane Flores

    2009-07-01

    Full Text Available Este estudo teve como objetivo otimizar a extração de ecdisterona em raízes de ginseng brasileiro. Primeiramente, para se avaliar a eficiência do solvente extrator, amostras de raízes dois acessos (BRA e JB-UFSM de P. glomerata foram extraídas em Soxhlet com metanol e clorofórmio, separadamente, durante 4 horas. No segundo ensaio, com o intuito de se escolher o método extrator, a extração foi conduzida em Soxhlet e em ultrassom, utilizando metanol como solvente. Em P. tuberosa, as amostras foram extraídas com metanol, e a extração foi conduzida em Soxhlet e em banho ultrasônico. O conteúdo de ecdisterona foi determinado em Cromatógrafo Líquido de Alta Eficiência (CLAE. Em ambas as espécies, um maior conteúdo de ecdisterona foi detectado nas amostras extraídas com metanol e em Soxhlet. A metodologia proposta mostrou-se eficaz para a quantificação da ecdisterona a partir das raízes de P. glomerata e P. tuberosa, podendo ser aplicada no controle de qualidade de drogas vegetais e/ou fitoterápicos.This study aimed at optimizing the extraction method from ecdysterone of Brazilian ginseng. Root samples of two accessions (BRA and JB-UFSM of P. glomerata were extracted in a Soxhlet with methanol or chloroform for 4h. In the second trial, the extration was conduced in a Soxhlet or ultrasonic using metanol as a solvent. In P. tuberosa, the roots samples were extracted with methanol in a Soxhlet or in ultrasonic. The ecdysterone content was determinated using high efficiency liquid chromatography methods. In both studied species, the highest ecdisterone content was detected from samples extracted in a Soxhlet and using methanol as a solvent. This extration method has been successfully applied for determination of ecdysterone content from roots of Brazilian ginseng, and could be useful for the quality control of drugs and pharmaceutical formulations.

  10. A histochemical study of root nodule development

    NARCIS (Netherlands)

    Wiel, van de C.

    1991-01-01

    In cooperation with soil bacteria of the genera <em>Rhizobium> , <em>Bradyrhizobium> or <em>Azorhizobium> , many members of the legume family are able to form specialized organs on their roots, called root nodules. The bacteria, wrapped up

  11. Intra-population plasticity of Anopheles darlingi's (Diptera, Culicidae biting activity patterns in the state of Amapá, Brazil

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    Voorham Jaco

    2002-01-01

    Full Text Available OBJECTIVE: To assess the variation in Anopheles darlingi's biting activity compared to An. marajoara in the same locality and to biting activity data from other regions. METHODS: Using human bait, eight observations of the biting activity of An. darlingi and An. marajoara were carried out during 1999 and 2000 in the municipality of São Raimundo do Pirativa, state of Amapá, Brazil. Each observation consisted of three consecutive 13-hour collections, close to full moon. There were shifts of collectors in the observation points and nocturnal periods. RESULTS: An. darlingi revealed considerable plasticity of biting activity in contrast to An. marajoara, which showed well-defined crepuscular biting peaks. No significant correlation between density and biting activity was found, but a significant correlation existed between time and proportional crepuscular activity, indicating underlying ecological processes not yet understood. Two of the four available data sets having multiple observations at one locality showed considerable plasticity of this species' biting patterns as well. CONCLUSION: Intra-population variation of biting activity can be as significant as inter-population variation. Some implications in malaria vector control and specific studies are also discussed.

  12. Intra-population plasticity of Anopheles darlingi's (Diptera, Culicidae) biting activity patterns in the state of Amapá, Brazil.

    Science.gov (United States)

    Voorham, Jaco

    2002-02-01

    To assess the variation in Anopheles darlingi's biting activity compared to An. marajoara in the same locality and to biting activity data from other regions. Using human bait, eight observations of the biting activity of An. darlingi and An. marajoara were carried out during 1999 and 2000 in the municipality of São Raimundo do Pirativa, state of Amapá, Brazil. Each observation consisted of three consecutive 13-hour collections, close to full moon. There were shifts of collectors in the observation points and nocturnal periods. An. darlingi revealed considerable plasticity of biting activity in contrast to An. marajoara, which showed well-defined crepuscular biting peaks. No significant correlation between density and biting activity was found, but a significant correlation existed between time and proportional crepuscular activity, indicating underlying ecological processes not yet understood. Two of the four available data sets having multiple observations at one locality showed considerable plasticity of this species' biting patterns as well. Intra-population variation of biting activity can be as significant as inter-population variation. Some implications in malaria vector control and specific studies are also discussed.

  13. Concurrent presence of Sarcocystis neurona sporocysts, Besnoitia darlingi tissue cysts, and Sarcocystis inghami sarcocysts in naturally infected opossums (Didelphis virginiana).

    Science.gov (United States)

    Elsheikha, H M; Fitzgerald, S D; Rosenthal, B M; Mansfield, L S

    2004-07-01

    Opossums (Didelphis virginiana) are exposed to a wide range of coccidia through feeding on a variety of foods, including, but not limited to, carrion, insects, and nestling birds. Abundant D. virginiana populations in urban and suburban areas can be important reservoirs of parasitic infection because of their profuse and prolonged excretion of the sporocysts of several species of Sarcocystis, their omnivorous diet, and their relatively long life span. This report describes 2 adult female opossums found to be simultaneously infected with the tissue cysts of Besnoitia darlingi, sarcocysts of Sarcocystis inghami, as well as with the intestinal sporocysts of S. neurona. Cysts typical of B. darlingi based on gross, histological, and ultrastructural characteristics were disseminated throughout the visceral organs, musculature, ears, and skin. The S. neurona and B. darlingi infections were confirmed by comparative sequence analysis of polymerase chain reaction-amplified diagnostic genetic loci. Sarcocysts of S. inghami are also described. Such examples of multiple parasitic infections show that concurrent infections occur naturally. The propensity for species to coexist should be considered in the differential diagnosis of tissue cyst-forming coccidian protozoa and may have important epidemiological and evolutionary implications.

  14. Enraizamento in vitro de mirtilo em condições fotoautotróficas In vitro rooting of blueberry under photoautotrophic conditions

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    Cláudia Roberta Damiani

    2009-07-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficiência do enraizamento fotoautotrófico de mirtilo (Vaccinium ashei Reade, cultivar 'Delite', comparando o efeito da luz natural (casa de vegetação e da luz artificial (sala de crescimento durante duas épocas do ano (verão e inverno, bem como o efeito de três concentrações de sacarose (0; 15 e 30g L-1 adicionadas ao meio de cultura e de diferentes tipos de vedação dos frascos de cultivo (alumínio, algodão e filme plástico. Após 60 dias de enraizamento in vitro em meio de cultura WPM, contendo 9µM L-1 de AIB, observou-se que o enraizamento em meio de cultura livre de sacarose promoveu o aumento do número de raízes e da porcentagem de enraizamento e, quando associado ao fechamento dos frascos com algodão, favoreceu, também, o incremento do comprimento das raízes e da massa fresca total. Quanto ao ambiente de cultivo e às épocas do ano, concluiu-se que o enraizamento pode ser realizado com sucesso de forma fotoautotrófica e, durante o verão, independentemente do ambiente de cultivo, verifica-se um aumento do comprimento de raízes, da porcentagem de enraizamento e da massa fresca total.The aim of this research was to assess the photoautotrofic rooting efficiency of blueberry (Vaccinium ashei Reade, cultivar 'Delite' by comparing the effect between natural light (greenhouse and artificial light (growth room in two seasons (summer and winter, as well as the effect of different three sucrose concentrations (0; 15 or 30g L-1 added to the culture medium and different types of flasks closure (aluminum, cotton and plastic film. After 60 days of in vitro rooting on WPM medium culture containing 9µM L-1 AIB, rooting in free-sucrose culture medium promoted an increased in the number of roots and rooting percentage; and, whether associated to cotton closed, there was an increase in length of roots and in the total fresh weight. Regarding environment growth and season, rooting could be

  15. Dynamical Mapping of Anopheles darlingi Densities in a Residual Malaria Transmission Area of French Guiana by Using Remote Sensing and Meteorological Data.

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    Antoine Adde

    Full Text Available Local variation in the density of Anopheles mosquitoes and the risk of exposure to bites are essential to explain the spatial and temporal heterogeneities in the transmission of malaria. Vector distribution is driven by environmental factors. Based on variables derived from satellite imagery and meteorological observations, this study aimed to dynamically model and map the densities of Anopheles darlingi in the municipality of Saint-Georges de l'Oyapock (French Guiana. Longitudinal sampling sessions of An. darlingi densities were conducted between September 2012 and October 2014. Landscape and meteorological data were collected and processed to extract a panel of variables that were potentially related to An. darlingi ecology. Based on these data, a robust methodology was formed to estimate a statistical predictive model of the spatial-temporal variations in the densities of An. darlingi in Saint-Georges de l'Oyapock. The final cross-validated model integrated two landscape variables-dense forest surface and built surface-together with four meteorological variables related to rainfall, evapotranspiration, and the minimal and maximal temperatures. Extrapolation of the model allowed the generation of predictive weekly maps of An. darlingi densities at a resolution of 10-m. Our results supported the use of satellite imagery and meteorological data to predict malaria vector densities. Such fine-scale modeling approach might be a useful tool for health authorities to plan control strategies and social communication in a cost-effective, targeted, and timely manner.

  16. Desempenho radicular de mudas de eucalipto produzidas em diferentes recipientes e substratos Root performace of eucalyptus stecklings, produced in different containers and substrates

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    Teresa A. S. de Freitas

    2005-12-01

    Full Text Available O trabalho teve como objetivo verificar o desempenho, em sacolas, de clones de eucalipto, produzidos em diferentes recipientes e substratos, com ênfase na persistência das deformações radiculares originadas no viveiro e na produção de raízes. As mudas foram produzidas em tubetes (50 cm³ e em blocos prensados (40 x 60 x 7 cm - 16.800 cm³, sendo utilizados como substratos: casca de arroz carbonizada com casca de eucalipto, bagaço de cana com torta de filtro; e turfa. As mudas, com 90 dias, foram transplantadas para sacos plásticos (20 L, com solo da área de plantio. O experimento foi conduzido em delineamento inteiramente casualizado, em esquema fatorial 2 x 7, constituído por dois clones (híbridos naturais de Eucalyptus grandis Hill ex Maiden e E. saligna Smith e sete tratamentos, com variação de recipientes e substrato, com quatro repetições, constituídas por quatro plantas. Dois meses após o transplantio, as plantas foram avaliadas quanto ao crescimento em diâmetro ao nível do solo, altura da parte aérea, número de raízes emitidas, comprimento, área superficial e deformação do sistema radicular. As plantas originadas de mudas produzidas em blocos prensados apresentaram melhor desempenho nas avaliações realizadas em relação às dos tubetes. As deformações radiculares causadas por recipientes de paredes rígidas tendem a persistir após a fase de viveiro.The objective of this work was to evaluate the performance after the planting of eucalyptus stecklings, produced in different containers and substrates, with emphasis in root production and deformations. The stecklings were produced in tubes (50 cm³ and in pressed blocks (40 x 60 x 7cm -16800 cm³, and used as substrates: carbonized rice husk and eucalyptus husk; sugarcane bagasse and sugarcane filter cake; and peat. At ninety days the stecklings were transplanted to plastic bags (20 L, with soil from the planting area. This experiment was set in a completely

  17. Enraizamento de manjericão em diferentes substratos e doses de cinzas Stem rooting of basil in different substrates and doses of ash

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    I.M. Silva

    2012-01-01

    Full Text Available O trabalho teve como objetivo avaliar a influência de doses de cinzas em diferentes substratos no enraizamento de estacas de manjericão (Ocimum basilicum. O experimento foi conduzido em área do Núcleo de Capacitação e Pesquisa da Universidade Federal Rural da Amazônia, em Belém. Foi utilizado o delineamento experimental inteiramente casualizado, em esquema fatorial 2 x 5, constituído pelas combinações entre os fatores substratos (casca de arroz carbonizada e casca de castanha triturada e acréscimo de cinzas (5 mL e 10 mL de cinza de casca de arroz, 5 mL e 10 mL de cinza de casca de castanha e sem cinza com 4 repetições. Cada unidade experimental foi constituída por 10 estacas, num total de 400 estacas. Foram avaliadas as características percentual de enraizamento de estacas, número e comprimento de raízes. Não houve interações significativas entre os fatores avaliados (substrato e cinzas. Entre as características avaliadas o substrato casca de arroz carbonizada apresentou diferenças apenas no comprimento da raiz e no número de raízes, enquanto as dosagens de cinzas apresentaram diferenças apenas no percentual de enraizamento. O trabalho permitiu concluir que as dosagens de cinzas apresentaram efeito positivo sobre o enraizamento das estacas de manjericão, assim como o substrato a base de casca de arroz carbonizada propiciou melhores condições para o desenvolvimento das raízes. Assim, a casca de arroz é uma alternativa totalmente viável, pois além de apresentar características apropriadas para o enraizamento também possui baixo custo de aquisição, estando ao alcance de muitos produtores.The study aimed to evaluate the influence of ash doses on different substrates on the stem rooting of basil (Ocimum basilicum. The experiment was conducted in the area of core training and research at the Universidade Federal Rural da Amazônia, in Belém, used a completely randomized design, factorial scheme 2 x 5 consisting

  18. Implications for changes in Anopheles darlingi biting behaviour in three communities in the peri-Iquitos region of Amazonian Peru.

    Science.gov (United States)

    Moreno, Marta; Saavedra, Marlon P; Bickersmith, Sara A; Lainhart, William; Tong, Carlos; Alava, Freddy; Vinetz, Joseph M; Conn, Jan E

    2015-07-30

    Malaria transmission in the peri-Iquitos region of Amazonian Peru has been designated as seasonal and hypo-endemic with recently described hyper-endemic hotspots. Despite relatively recent distribution of long-lasting insecticidal bed nets (LLINs), malaria in Amazonian Peru persists and increased substantially in 2014 compared to previous years. Anopheles darlingi, identified as the main malaria vector, is known for its variable behaviour depending on locality and environment. To evaluate vector biology metrics in relation to seasonality and malaria transmission, mosquito collections were carried out in three localities in the peri-Iquitos region, Loreto, Peru in 2011-2012. Human landing catch (HLC) collection method, Shannon (SHA) and CDC trap types were compared for effectiveness in a neotropical setting. Abundance, human biting rate and entomological inoculation rate (EIR) were measured to provide an updated view of transmission patterns post-LLIN distribution. HLC collected significantly more anopheline mosquitoes than SHA and CDC light traps. Anopheles darlingi was the most prevalent species in all three villages (84% overall). Biting patterns varied depending on trap type, season and village. EIR varied temporally (monthly) and spatially and the highest (2.52) occurred during the 2012 malaria outbreak in Cahuide. Unexpectedly there was a high infection rate (1.47 and 1.75) outside the normal malaria transmission season, coincident with a second local outbreak in Cahuide. The first identification of Anopheles dunhami and Anopheles oswaldoi C in Peru, using molecular markers, is also reported in this study. These data underscore the importance of HLC as the most meaningful collection method for measuring vector biology indices in this region. The highest monthly EIR provides additional evidence of seasonal transmission in riverine localities correlated with high river levels, and An. darlingi as the only contributor to transmission. The trend of an increase in

  19. Habitat suitability mapping of Anopheles darlingi in the surroundings of the Manso hydropower plant reservoir, Mato Grosso, Central Brazil

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    Miyazaki Rosina D

    2007-03-01

    Full Text Available Abstract Background Hydropower plants provide more than 78 % of Brazil's electricity generation, but the country's reservoirs are potential new habitats for main vectors of malaria. In a case study in the surroundings of the Manso hydropower plant in Mato Grosso state, Central Brazil, habitat suitability of Anopheles darlingi was studied. Habitat profile was characterized by collecting environmental data. Remote sensing and GIS techniques were applied to extract additional spatial layers of land use, distance maps, and relief characteristics for spatial model building. Results Logistic regression analysis and ROC curves indicate significant relationships between the environment and presence of An. darlingi. Probabilities of presence strongly vary as a function of land cover and distance from the lake shoreline. Vector presence was associated with spatial proximity to reservoir and semi-deciduous forests followed by Cerrado woodland. Vector absence was associated with open vegetation formations such as grasslands and agricultural areas. We suppose that non-significant differences of vector incidences between rainy and dry seasons are associated with the availability of anthropogenic breeding habitat of the reservoir throughout the year. Conclusion Satellite image classification and multitemporal shoreline simulations through DEM-based GIS-analyses consist in a valuable tool for spatial modeling of A. darlingi habitats in the studied hydropower reservoir area. Vector presence is significantly increased in forested areas near reservoirs in bays protected from wind and wave action. Construction of new reservoirs under the tropical, sub-humid climatic conditions should therefore be accompanied by entomologic studies to predict the risk of malaria epidemics.

  20. Population genetic structure of the major malaria vector Anopheles darlingi (Diptera: Culicidae from the Brazilian Amazon, using microsatellite markers

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    Vera Margarete Scarpassa

    2007-06-01

    Full Text Available The population genetic structure of Anopheles darlingi, the major human malaria vector in the Neotropics, was examined using seven microsatellite loci from nine localities in central and western Amazonian Brazil. High levels of genetic variability were detected (5-25 alleles per locus; H E = 0.519-0.949. There was deviation from Hardy-Weinberg Equilibrium for 59.79% of the tests due to heterozygote deficits, while the analysis of linkage disequilibrium was significant for only two of 189 (1.05% tests, most likely caused by null alleles. Genetic differentiation (F ST = 0.001-0.095; Nm = 4.7-363.8 indicates that gene flow is extensive among locations < 152 km apart (with two exceptions and reduced, but not absent, at a larger geographic scale. Genetic and geographic distances were significantly correlated (R² = 0.893, P < 0.0002, supporting the isolation by distance (IBD model. The overall estimate of Ne was 202.4 individuals under the linkage disequilibrium model, and 8 under the heterozygote excess model. Analysis of molecular variance showed that nearly all variation (~ 94% was within sample locations. The UPGMA phenogram clustered the samples geographically, with one branch including 5/6 of the state of Amazonas localities and the other branch the Acre, Rondônia, and remaining Amazonas localities. Taken together, these data suggest little genetic structure for An. darlingi from central and western Amazonian Brazil. These findings also imply that the IBD model explains nearly all of the differentiation detected. In practical terms, populations of An. darlingi at distances < 152 km should respond similarly to vector control measures, because of high gene flow.

  1. Dried chicory root modifies the activity and expression of porcine hepatic CYP3A but not 2C – Effect of <em>in vitroem> and<em> in vivoem> exposure

    DEFF Research Database (Denmark)

    Rasmussen, Martin Krøyer; Zamaratskaia, Galia; Andersen, Bente

    2012-01-01

    Hepatic cytochrome P450 expression and activity are dependent on many factors, including dietary ingredients. In the present study, we investigated the in vivo and in vitro effect of chicory root on hepatic CYP3A and 2C in male pigs. Chicory feeding increased the expression of CYP3A29 mRNA but no...

  2. Serological response of cats to experimental Besnoitia darlingi and Besnoitia neotomofelis infections and prevalence of antibodies to these parasites in cats from Virginia and Pennsylvania

    Science.gov (United States)

    Besnoitia darlingi and B. neotomofelis are tissue cyst-forming apicomplexan parasite that use domestic cats (Felis domesticus) as definitive hosts and opossums (Didelphis virginiana) and southern planes woodrats (Neotoma micropus) as intermediate hosts, respectively. Nothing is known about the preva...

  3. Copaifera multijuga ethanolic extracts, oil-resin, and its derivatives display larvicidal activity against Anopheles darlingi and Aedes aegypti (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    Frances Tatiane Tavares Trindade

    2013-06-01

    Full Text Available Copaifera spp. is a common tree species found in the tropical region of Latin America, popularly known as copaiba or pau-d'alho. Oil-resin from different Copaifera species and its components present several biological activities such as antimicrobial, anti-inflammatory, antioxidant and insecticidal, including larvicidal activity against mosquitoes. Thus, bark and leaf ethanolic extracts, oil-resin, essential oil and alepterolic acid from Copaifera multijuga Hayne, Fabaceae, were tested as larvicides against the main malaria vector in the north of Brazil, Anopheles darlingi and also Aedes aegypti, the dengue vector. A. darlingi larval mortality was significantly higher than A. aegypti for most tested compounds. Bark and leaf extracts resulted in lower Lethal Concentrations (LC50 values for A. darlingi, 3 and 13 ppm, respectively, while the essential oil provided the lowest LC50 value for A. aegypti, 18 ppm. Despite of that, the lowest LC values were from the alepterolic acid for both species, i.e. 0.9 and 0.7 ppm for A. darlingi and A. aegypti, respectively.

  4. Copaifera multijuga ethanolic extracts, oil-resin, and its derivatives display larvicidal activity against Anopheles darlingi and Aedes aegypti (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    Frances Tatiane Tavares Trindade

    2013-05-01

    Full Text Available Copaifera spp. is a common tree species found in the tropical region of Latin America, popularly known as copaiba or pau-d'alho. Oil-resin from different Copaifera species and its components present several biological activities such as antimicrobial, anti-inflammatory, antioxidant and insecticidal, including larvicidal activity against mosquitoes. Thus, bark and leaf ethanolic extracts, oil-resin, essential oil and alepterolic acid from Copaifera multijuga Hayne, Fabaceae, were tested as larvicides against the main malaria vector in the north of Brazil, Anopheles darlingi and also Aedes aegypti, the dengue vector. A. darlingi larval mortality was significantly higher than A. aegypti for most tested compounds. Bark and leaf extracts resulted in lower Lethal Concentrations (LC50 values for A. darlingi, 3 and 13 ppm, respectively, while the essential oil provided the lowest LC50 value for A. aegypti, 18 ppm. Despite of that, the lowest LC values were from the alepterolic acid for both species, i.e. 0.9 and 0.7 ppm for A. darlingi and A. aegypti, respectively.

  5. Collapse of Anopheles darlingi populations in Suriname after introduction of insecticide-treated nets (ITNs); malaria down to near elimination level

    NARCIS (Netherlands)

    Hiwat, H.; Mitro, S.; Samjhawan, A.; Sardjoe, P.; Soekhoe, T.; Takken, W.

    2012-01-01

    A longitudinal study of malaria vectors was carried out in three villages in Suriname between 2006 and 2010. During 13,392 man hours of collections, 3,180 mosquitoes were collected, of which 33.7% were anophelines. Of these, Anopheles darlingi accounted for 88.1%, and An. nuneztovari accounted for

  6. Succession of root-associated fungi in <em>Pisum sativum em>during a plant growth cycle as examined by 454 pyrosequencing

    DEFF Research Database (Denmark)

    Yu, L.; Nicolaisen, M.; Larsen, J.

    2012-01-01

    Purpose Roots are inhabited by a broad range of fungi, including pathogens and mycorrhizal fungi, with functional traits related to plant health and nutrition. Management of these fungi in agroecosystems requires profound knowledge about their ecology. The main objective of this study was to exam...

  7. Growth of bare root Pinus taeda, L. seedlings cultivated under five densities in nursery Crescimento de mudas em raiz nua de Pinus taeda, L. produzidas em cinco densidades no viveiro

    Directory of Open Access Journals (Sweden)

    José Geraldo de Araújo Carneiro

    2007-02-01

    Full Text Available Seedlings compete for nutrients, water and light. The available area for each seedling affects their behavior related to requirements for these resources. This experiment evaluated the influence of five plant densities on the growth of bare root Pinus taeda, L. seedlings in a nursery after outplanting. The analyzed characteristics were: height (H, root collar diameter (D, H/D ratio, and dry matter weight. Higher densities stimulated H growth and the lowest densities increased D average and dry matter weight and lowered the H/D ratio. Seedlings were distributed by H, D and H/D classes. Higher densities had a larger number of seedlings in larger H classes. Larger numbers of seedlings with larger D and lower H/D ratios were found in lower densities. Ten months after outplanting the seedlings grown in lower densities had higher survival percentages and growth. Some saplings of standardized heights were uprooted with the objective of studying their root systems. The lowest densities stimulated higher numbers of first and second order roots as well as fresh and dry matter weights of thin roots with mycorrhizae presence. In both parts of the experiment, the density of 278 seedlings m-2 yielded equivalent averages as compared to the lowest densities.Mudas competem por nutrientes, água e luz. A área de cada muda afeta seu comportamento na exigência destes recursos. Este experimento avaliou a influência de cinco densidades no crescimento de mudas em raiz nua de Pinus taeda, L. no viveiro e no campo. As características analisadas foram: altura da parte aérea (H, diâmetro de colo (D, relação H/D e pesos de matéria seca. Maiores densidades estimularam o crescimento em H, no viveiro. As mais baixas aumentaram as médias de D, pesos de matéria seca e diminuíram a relação H/D. Distribuíram-se as mudas em classes de H, D e relação H/D, para quantificar seu número, em cada classe. Maiores quantidades de mudas com mais elevados valores de D e com

  8. Flutuação populacional de curculionídeos-das-raízes em citros Population dynamics of root weevils in citrus

    Directory of Open Access Journals (Sweden)

    Jerson Vanderlei Carús Guedes

    2009-06-01

    Full Text Available O objetivo neste trabalho foi estudar a flutuação populacional de curculionídeos-das-raízes em citros. Foram feitas amostragens quinzenais das fases de larva, de pupa e adulta, em laranjeiras de quatro fazendas, em Itapetininga, São Paulo, de março de 2000 a fevereiro de 2002. A emergência dos adultos foi quantificada em gaiolas sob a copa das plantas e a população de adultos, na copa das plantas, com pano-de-batida; as larvas e pupas em trincheiras abertas no solo, sob a copa das plantas. Com base na flutuação populacional das fases, é possível afirmar que os insetos são univoltinos. A maioria dos adultos emergiu de outubro a abril, com picos no início do período. Foram encontradas as espécies Parapantomorus fluctuosus (Boheman, Naupactus cervinus Boheman e Naupactus versatilis Hustache, sendo as duas últimas as mais freqüentes, embora com picos em épocas diferentes. Ocorreram larvas praticamente o ano inteiro, com picos populacionais no período de junho a janeiro. A população de curculionídeos foi menor no segundo ano de estudo, provavelmente em conseqüência da estiagem. Dentre as fases estudadas, os adultos e as larvas, amostrados com pano-de-batida e em trincheiras no solo, podem servir para detecção, quantificação e previsão da ocorrência dos curculionídeos-das-raízes dos citros.The goal of this study was to evaluate the population dynamics of citrus root weevils. Samples of the larval, pupa and adult phases were taken every 15 days in four citrus farms in Itapetininga, State of São Paulo, Brazil, between March, 2000 and February, 2002. The emergence of adults was evaluated in cages under plant canopy and the adult population at plant canopy with a beating cloth; larvae and the pupae in ditches opened in the soil, under plant crowns. Based on the population dynamics, the insects are univoltine. Most adults emerged from October to April, with peaks at the beginning of the period. Species Parapantomorus

  9. Management of chili pepper root rot and wilt (caused by <em>Phytophthora nicotianaeem>) by grafting onto resistant rootstock

    OpenAIRE

    Mourad SAADOUN; Mohamed Bechir ALLAGUI

    2013-01-01

    Root rot and plant wilting caused by Phytophthora nicotianae is a severe disease of chili pepper (Capsicum annuum L.) in open fields and under greenhouse production in Tunisia. Chili pepper grafting for disease manage- ment is attracting increased interest in recent years. Using the tube grafting technique, different compatible scion/rootstock combinations were obtained with the wild-type pepper SCM334 and the local chili pepper cultivars ‘Beldi’ and ‘Baker’. SCM334 was resistant to P. nicoti...

  10. Desenvolvimento radicular do algodoeiro em resposta à localização do fertilizante Cotton root development as affected by fertilizer placement

    Directory of Open Access Journals (Sweden)

    Fábio Suano de Souza

    2007-04-01

    Full Text Available A utilização de adubos pode-se tornar prejudicial caso o fertilizante não seja localizado adequadamente. No presente trabalho foram estudados o crescimento radicular do algodoeiro (Gossypium hirsutum, o crescimento inicial e a nutrição da planta, considerando o local de aplicação do fertilizante. O estudo foi realizado em vasos com parede de vidro. O fertilizante foi colocado a 5,0 cm abaixo e 0,0, 2,5, 5,0 e 10,0 cm ao lado das sementes. O crescimento radicular foi avaliado a cada três dias e, aos 21 dias após a emergência, as plantas foram coletadas, sendo avaliada a produção de matéria seca e a absorção de macronutrientes. A aplicação de fertilizante proporcionou crescimento inicial mais vigoroso do sistema radicular mesmo em solo previamente corrigido e adubado, o que é importante no estabelecimento da cultura. Somente houve bom crescimento inicial do sistema radicular e da parte aérea do algodoeiro quando o fertilizante foi aplicado de 5,0 a 10,0 cm ao lado e 5,0 cm abaixo das sementes.Unless fertilizer is properly placed in the soil it can be harmful. This experiment was conducted to study cotton (Gossypium hirsutum root growth and initial plant development and nutrition as affected by fertilizer placement. Cotton plants were grown in pots with a glass wall. The fertilizer was applied 5.0 cm under the seed row and 0, 2.5, 5.0 and 10.0 cm beside the seed row. Root growth was evaluated every 3 days, and 21 days after emergence the plants were harvested. Dry matter production and macronutrient absorption were evaluated. Even in previously limed and fertilized soil, localized fertilizer application reinforced the initial growth of cotton roots, which is very important for a good crop establishment in the field. Normal root growth and adequate initial plant development was only observed when the fertilizer was placed 5.0 cm below and from 5.0 to 10.0 cm distance from the seed row.

  11. Sistema radicular dos citros em Neossolo Quartzarênico dos Tabuleiros Costeiros sob irrigação e sequeiro Citrus root system in Coastal Tablelands on Typic Quartzipsamment under irrigation and rainfed conditions

    Directory of Open Access Journals (Sweden)

    Laercio Duarte Souza

    2007-10-01

    Full Text Available O objetivo deste trabalho foi avaliar o sistema radicular da laranjeira 'Pêra' Citrus sinensis (L. Osb., enxertada em limoeiro 'Rugoso' Citrus jambhiri Lush., plantada no espaçamento 6x4 m, sob sistema irrigado com microaspersores e sob sequeiro. O solo da área experimental, localizada em Itapicuru, BA, é um Neossolo Quartzarênico, com horizonte subsuperficial coeso. O delineamento experimental foi o inteiramente casualizado, e foram avaliadas cinco plantas em cada sistema. Amostras de raiz foram coletadas a cada 0,5 m de distância do caule, em seis profundidades, em quatro locais, na linha de plantas e seis nas entrelinhas. A distribuição das raízes foi avaliada em três classes de diâmetro (1 mm. A comparação entre os locais, em relação à planta, nos sistemas irrigado e sequeiro, tanto na linha de plantas quanto na entrelinha, apresentou diferenças na distribuição de raízes apenas à profundidade de 0-0,20 m. As 36 combinações dos três diâmetros de raiz e das seis profundidades, entre os sistemas irrigado e sequeiro, mostraram diferenças na distribuição de raízes apenas no diâmetro 0,5 e The aim of this work was to evaluate the root system of 'Pera' orange tree Citrus sinensis (L. Osb. grafted on 'Rugoso' lemon tree Citrus jambhiri Lush., cultivated with spacing within and between rows of 4 and 6 m, respectively, under rainfed conditions and under irrigation. The experimental area was established in Itapicuru, BA, Brazil, in a Typic Quartzipsamment soil with a subsuperficial cohesive layer. The experimental was a completely randomized design with five plants evaluated at each system. Root samples were collected in six positions between rows and four positions within rows, spaced of 0.5 m from tree's stem. At each position, the roots were sampled in six depths. Root distribution was evaluated using three ranges of root diameters (1 mm. Comparing the positions from the plants, within and between rows in both systems

  12. Utilização de Gigaspora margarita em plantas micropropagadas de bananeira em diferentes estádios de enraizamento The use of arbuscular mycorrhizal fungi Gigaspora margarita in micropropagated bananas plants in differents stages of rooting

    Directory of Open Access Journals (Sweden)

    Gêlva Maria de Lima Lins

    2003-04-01

    Full Text Available Visando a avaliar o efeito de fungos micorrízicos arbusculares (FMA na diminuição do tempo de formação de mudas de bananeira micropropagadas, foi conduzido um experimento em estufa de aclimatação da Biofábrica CAMPO - CPA/Embrapa Mandioca e Fruticultura, Cruz das Almas, Bahia. Foram testadas plântulas em três diferentes estádios de desenvolvimento do sistema radicular, inoculadas ou não com o fungo Gigaspora margarita e cultivadas em dois diferentes substratos à base de turfa, vermiculita e esterco. A inoculação foi realizada no momento do transplantio para a estufa e as plantas, cultivadas por 55 dias, quando foram coletadas para obtenção dos dados de avaliação. O fungo Gigaspora margarita colonizou intensamente e mostrou- se benéfico para o desenvolvimento das mudas de bananeira, sendo seu efeito modulado pelo substrato de crescimento; o substrato turfa + vermiculita + 5% de esterco, quando associado à inoculação do FMA, promoveu a formação de mudas normais e sadias; o início da fase de aclimatação de mudas micropropagadas de bananeira pode ser antecipado pelo uso da inoculação com fungos micorrízicos arbusculares, em substrato adequado.With the aim of evaluate de use of arbuscular mycorrhizal fungi Gigaspora margarita to reduce the time of formation of micropropagated banana plants it was conducted an experiment under greenhouse conditions at the CAMPO-CPA (micropropagating plant/Embrapa-Brazilian Agricultural Research Corporation, in the city of Cruz das Almas, State of Bahia. Plants were tested at three different stages of formation: unrooted, half-rooted and completed rooted being cultivated on two substrates composed from mistures of turfgrass, vermiculite and manure. The plants were inoculated at the time of transplanting to the different substrates and were harvested after the acclimatization period of 55 days, for evaluation of its growth, mineral nutrition and mycorrhizal colonization. Gigaspora

  13. Root rot peas in the Netherlands : fungal pathogens, inoculum potential and soil receptivity

    NARCIS (Netherlands)

    Oyarzun, P.J.

    1994-01-01

    Fungi associated to pea <em>(Pisum sativumem> L.) <em>> root rot were studied. <em>Fusarium> and Oomycetes were most common. <em>Fusarium solaniem> f. sp. <em>pisi,> Fsp, was widely distributed and the most frequent

  14. Changes in anatomy and root cell ultrastructure of soybean genotypes under manganese stress Alterações anatômicas e ultraestruturais em genótipos de soja pela desordem nutricional em manganês

    Directory of Open Access Journals (Sweden)

    José Lavres Junior

    2009-04-01

    Full Text Available The deleterious effects of both Mn deficiency and excess on the development of plants have been evaluated with regard to aspects of shoot anatomy, ultrastructure and biochemistry, focusing mainly on the manifestation of visual symptoms. However, there is little information in the literature on changes in the root system in response to Mn supply. The objective of this study was to evaluate the effects of Mn doses (0.5, 2.0 and 200.0 μmol L-1 in a nutrient solution on the anatomy of leaves and roots of the Glycine max (L. cultivars Santa Rosa, IAC-15 and IAC-Foscarin 31. Visual deficiency symptoms were first observed in Santa Rosa and IAC-15, which were also the only cultivars where Mn-toxicity symptoms were observed. Only in IAC-15, a high Mn supply led to root diameter thickening, but without alteration in cells of the bark, epidermis, exodermis and endodermis. The degree of disorganization of the xylem vessels, in particular the metaxylem, differed in the cultivars. Quantity and shape of the palisade parenchyma cells were influenced by both Mn deficiency and toxicity. A reduction in the number of chloroplasts was observed in the three Mn-deficient genotypes. The anatomical alterations in IAC-15 due to nutritional stress were greater, as expressed in extensive root cell cytoplasm disorganization and increased vacuolation at high Mn doses. The degree of changes in the anatomical and ultrastructural organization of roots and leaves of the soybean genotypes studied differed, suggesting the existence of tolerance mechanisms to different intensities of Mn deficiency or excess.Os efeitos negativos provocados não apenas pela deficiência mas também pela toxidez de Mn no desenvolvimento das plantas têm sido avaliados considerando-se os aspectos anatômicos, de ultraestrutura e bioquímicos da parte aérea particularmente, onde os sintomas visuais são manifestados. Entretanto, há escassez na literatura de informações que abordem o sistema

  15. Root rots

    Science.gov (United States)

    Kathryn Robbins; Philip M. Wargo

    1989-01-01

    Root rots of central hardwoods are diseases caused by fungi that infect and decay woody roots and sometimes also invade the butt portion of the tree. By killing and decaying roots, root rotting fungi reduce growth, decrease tree vigor, and cause windthrow and death. The most common root diseases of central hardwoods are Armillaria root rot, lnonotus root rot, and...

  16. Population Structure of the Primary Malaria Vector in South America, Anopheles darlingi, Using Isozyme, Random Amplified Polymorphic DNA, Internal Transcribed Spacer 2, and Morphologic Markers

    Science.gov (United States)

    1999-01-01

    401 16 to Jan E. Conn for the ITS2 sequencing, by Consejo National de Investigaciones Cientificas y Tecnologicas (MPS-RPIV-130032-9), and the Pan... Investigaciones , Escuela de Malariologia y Saneamiento Ambiental, Maracay, Venezuela Abstract. A genetic and morphologic survey of Anopheles darlingi...0086. Yasmin Rubio-Palis, Division de Investigaciones , Escuela de Malariologia y Saneamiento Ambiental Dr. Arnold0 Ga- baldon, Apartado 2073

  17. Atividade do sistema antioxidante e desenvolvimento de aerênquima em raízes de milho 'Saracura' Antioxidant system activity and aerenchyma formation in 'Saracura' maize roots

    Directory of Open Access Journals (Sweden)

    Fabricio José Pereira

    2010-05-01

    Full Text Available Este trabalho teve como objetivo avaliar a influência de sucessivos ciclos de seleção do milho 'Saracura' na atividade das enzimas do sistema antioxidante, e a relação dessas enzimas com a capacidade dessa variedade em desenvolver aerênquima. Sementes de 18 ciclos de seleção intercalados do milho 'Saracura' e da cultivar BR 107, sensível à hipoxia, foram semeadas em vasos e em casa de vegetação. As plantas foram submetidas ao alagamento intermitente de dois em dois dias. As amostras de raízes foram coletadas após 60 dias e analisaram-se as atividades das enzimas peroxidase do guaiacol, peroxidase do ascorbato e catalase, além da capacidade das plantas de cada ciclo desenvolverem aerênquima. Ao longo dos ciclos, as plantas apresentaram modificações na atividade das enzimas, com aumento na de peroxidase do ascorbato e diminuição na de catalase e de peroxidase do guaiacol. Observou-se, ainda, maior capacidade de desenvolver aerênquima nos últimos ciclos de seleção. A redução na atividade das enzimas do sistema antioxidante parece estar relacionada a um desbalanço na decomposição de H2O2.This work aimed to assess the influence of successive selection cycles in 'Saracura' maize on the enzyme activity of the antioxidant system and the relationship of these enzymes with the aerenchyma development capacity of this variety. Seeds of 18 intercalated selection cycles of the 'Saracura' maize and of the cultivar BR 107, sensitive to hipoxia, were sown in pots in the greenhouse. Plants were submitted to intermittent soil flooding each two days. After 60 days, the roots were sampled and analysis were done for the guaiacol peroxidase, ascorbate peroxidase, and catalase activities and for the capacity of the plants of each cycle to develop aerenchyma. The plants showed modifications in enzyme activity along the cycles, increasing the ascorbate peroxidase activity and decreasing the catalase and guaiacol peroxidase ones. A greater

  18. Establishment of Besnoitia darlingi from opossums (Didelphis virginiana) in experimental intermediate and definitive hosts, propagation in cell culture, and description of ultrastructural and genetic characteristics.

    Science.gov (United States)

    Dubey, J P; Lindsay, D S; Rosenthal, B M; Sreekumar, C; Hill, D E; Shen, S K; Kwok, O C H; Rickard, L G; Black, S S; Rashmir-Raven, A

    2002-07-01

    Besnoitia darlingi from naturally infected opossums (Didelphis virginiana) from Mississippi, USA, was propagated experimentally in mice, cats, and cell culture and was characterised according to ultrastructural, genetic, and life-history characteristics. Cats fed tissue cysts from opossums shed oocysts with a prepatent period of nine or 11 days. Oocysts, bradyzoites, or tachyzoites were infective to outbred and interferon-gamma gene knockout mice. Tachyzoites were successfully cultivated and maintained in vitro in bovine monocytes and African green monkey cells and revived after an 18-month storage in liquid nitrogen. Schizonts were seen in the small intestinal lamina propria of cats fed experimentally-infected mouse tissues. These schizonts measured up to 45 x 25 microm and contained many merozoites. A few schizonts were present in mesenteric lymph nodes and livers of cats fed tissue cysts. Ultrastructurally, tachyzoites and bradyzoites of B. darlingi were similar to other species of Besnoitia. A close relationship to B. besnoiti and an even closer relationship to B. jellisoni was indicated for B. darlingi on the basis of the small subunit and ITS-1 portions of nuclear ribosomal DNA.

  19. Collapse of Anopheles darlingi populations in Suriname after introduction of insecticide-treated nets (ITNs); malaria down to near elimination level.

    Science.gov (United States)

    Hiwat, Hélène; Mitro, Sutrisno; Samjhawan, Ashok; Sardjoe, Prem; Soekhoe, Treyanti; Takken, Willem

    2012-04-01

    A longitudinal study of malaria vectors was carried out in three villages in Suriname between 2006 and 2010. During 13,392 man hours of collections, 3,180 mosquitoes were collected, of which 33.7% were anophelines. Of these, Anopheles darlingi accounted for 88.1%, and An. nuneztovari accounted for 11.1%. The highest mean An. darlingi human biting rate (HBR) observed per survey was 1.43 bites/man per hour outdoor and 1.09 bites/man per hour indoor; 2 An. darlingi of the 683 tested were infected with Plasmodium falciparum. The anopheline HBR decreased to zero after the onset of malaria intervention activities, including insecticide-treated net (ITN) distribution, in 2006. Malaria transmission decreased to pre-elimination levels. It is concluded that the combination of ITN and climatic events has led to the collapse of malaria vector populations in the study sites in the interior of the country. The results are discussed in relation to the stability of malaria transmission in areas with low-density human populations.

  20. Enraizamento in vitro de um porta-enxerto de macieira em diversos substratos In vitro rooting of an apple rootstock in several substrates

    Directory of Open Access Journals (Sweden)

    Moacir Pasqual

    2000-12-01

    Full Text Available O processo tradicional para produção de porta-enxertos de macieira é o de mergulhia, que apresenta baixa eficiência. As técnicas de cultura de tecidos têm sido uma alternativa viável, pois permitem aumentar o rendimento no processo de multiplicação, evitam disseminação de doenças e mantém as características da planta mãe. O presente trabalho teve como objetivo identificar um substituto do ágar no meio de cultivo para o enraizamento do porta-enxerto de macieira (Malus domestica Borkh. cv. M-7. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 5x4 com 4 repetições e 4 explantes por parcela. As concentrações dos sais do meio MS utilizadas foram 0%, 50%, 100%, 150% e 200% e os substratos foram ágar (3,0 e 6,0 g L-1, vermiculita e areia, em todas as combinações possíveis. Em todos os tratamentos o meio de cultura MS foi suplementado com 1,0 mg L-1 de IBA. As avaliações foram efetuadas 45 dias após a inoculação através dos seguintes parâmetros: altura de brotos, peso da matéria fresca e seca da parte aérea e do sistema radicular. Constatou-se que o melhor desenvolvimento da parte aérea e sistema radicular é obtido com o uso de ágar 3,0 e 6,0 g L-1, independentemente da concentração de sais. O uso de areia apresenta resultados similares ao ágar quando a concentração de sais é de 100% do meio MS.The traditional process for production of apple rootstocks presents low efficiency. The tissue culture techniques have been a viable alternative, because they allow to increase the multiplication process, they avoid dissemination of diseases and maintains the plant mother's characteristics. The objective of this work is identify a substitute for agar in the growth media for the rooting of apple (Malus domestica Borkh. rootstock, cultivar M-7. The experimental design was completely randomized, in a 5x4 factorial scheme, with 4 replications and 4 explants per plot. The salt concentrations of the

  1. The effect of flowering on adventitious root-formation

    NARCIS (Netherlands)

    Selim, H.H.A.

    1956-01-01

    The rooting of cuttings from <em>day-neutral tomatoem> was not influenced by flower development, nor by SD or LD treatments of them or of the mother plants. In cuttings of the SD plant <em>Perilla crispaem> flower initiation and development severely inhibited rooting. Leaves produced about 61 %

  2. Enraizamento de estacas de azaleia (Rhododendron simsii Planch. no outono em AIB e diferentes substratos Rooting of azalea cuttings (Rhododendron simsii Planch. in the fall of AIB and different substrates

    Directory of Open Access Journals (Sweden)

    Alessandro Borini Lone

    2010-08-01

    Full Text Available A azaleia é uma planta ornamental propagada comercialmente por meio de estacas, sendo difícil o seu o enraizamento em muitos casos, o que resulta em baixa produção de mudas. O objetivo do trabalho foi avaliar o uso do AIB e o efeito de diferentes substratos sobre o enraizamento de estacas herbáceas de azaleia no outono. O delineamento experimental foi o inteiramente casualizado, com cinco repetições, em um fatorial 2x3, sendo adotados os fatores: aplicação ou não de ácido indol butírico - AIB (0mg L-1 e 1.000mg L-1 e tipos de substratos (vermiculita - grânulos médios, casca de arroz carbonizada e fibra de coco. Após 76 dias, foram avaliadas as seguintes variáveis: retenção foliar (%, estacas enraizadas (%, sobrevivência das estacas (%, comprimento da maior raiz, número de folhas, massa de matéria fresca das raízes por estaca, pH e condutividade elétrica dos substratos. Os resultados mostraram não haver diferenças para o enraizamento pela aplicação ou não de AIB. As estacas enraizadas em casca de arroz carbonizada e vermiculita apresentaram número superior de folhas em relação às estacas do substrato fibra de coco. No entanto, a fibra de coco e a casca de arroz carbonizada proporcionaram um maior comprimento de raiz. Para massa de matéria fresca por estaca, a fibra de coco também apresentou o melhor resultado. Não houve contribuição do AIB no enraizamento de estacas de azaleia, e o substrato fibra de coco foi o que apresentou melhor desempenho para o enraizamento das estacas.Azalea is an ornamental plant commercially propagated by cuttings, and their rooting process in many cases, is difficult, which results in decreased production of seedlings. The objective of this study was to evaluate the use of IBA and the effect of different substrates on the rooting of cuttings of azalea in the fall. A randomized design with five replications in a factorial array was used as a statistical model, and it was adopted the

  3. Avaliação da porosidade e placa férrica de raízes de arroz cultivado em hipoxia Evaluation of porosity and iron plaque on rice roots grown under hypoxia

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    Marquel Jonas Holzschuh

    2010-10-01

    servir como estimativa da formação de aerênquima no arroz. O método de obtenção de raízes foi eficiente em promover a eliminação de O2 do saco de vinil para estudar a formação do aerênquima.The high oxygen diffusion in different materials makes the establishment and maintenance of oxygen-free environments difficult. The techniques used to obtain oxygen-free environments are little efficient and not representative of flooded soil conditions. The purpose of this study was to develop a method for obtaining roots in a hypoxic environment to evaluate iron plaque and aerenchyma formation in rice plants. A hypoxic condition similar to that of flooded soils was created in 50 L tanks, based on the oxygen diffusion capacity through vinyl plastic in contact with flooded soil. Each tank was filled with soil (Gley soil, five vinyl bags and then flooded. Rice plants of the genotypes IRGA 423 and IRGA 424 grown in the field were collected, the roots cut at the stem, washed and 1/3 of the leaves removed. Each bag was filled with 12 plants of each genotype and nutrient solution. After seven days, the new adventitious roots were used to determine the iron plaque and aerenchyma formation in the segments 0-2, 2-4 and 4-6 cm from the root tips. The roots were exposed the solution of a flooded soil for 4 hours. The iron plaque was determined in the root segments after iron extraction with HCl 0.5 mol L-1. Porosity was determined in vacuum cycles applied with a syringe. The weight difference before and after vacuum treatment and water entrance was assumed as estimate of the magnitude of the aerenchyma of the roots. The method was tested with the production of new adventitious roots in vinyl bags under aerated and hypoxic conditions. Root porosity was higher in the hypoxic than in the aerated environment. Porosity was higher near plant base and as porosity increased, iron increased near the root surface, indicating the iron plaque as a parameter to estimate aerenchyma formation in

  4. Radiographic prevalence of root canal ramifications in a sample of root canal treatments in a Brazilian Dental School Prevalência radiográfica de ramificações do canal radicular em uma amostra de tratamentos endodônticos em uma Faculdade de Odontologia Brasileira

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    Iadasa de Quadros

    2007-06-01

    Full Text Available The aim of this study was to radiographically investigate the presence of root canal ramifications found after endodontic treatment, and to determine any relationship between their presence and the type of the auxiliary chemical substance used. The study evaluated 1,470 endodontic treatments performed by final year undergraduate students at the Dental School of Piracicaba, State University of Campinas (UNICAMP, SP, Brazil, during the period from 1998 to 2000. The X-rays taken during treatment were evaluated in order to establish the presence of ramifications of the root canal system. The initial X-ray did not show the presence of any canal ramifications. After filling, X-rays showed only 3 ramification types: 3.06% of lateral canals, 2.99% of apical deltas, and 0.1% of interradicular canals. The maxillary premolars showed the highest number of lateral canals (n = 13, followed by mandibular premolars (n = 10 and maxillary incisors (n = 10. Apical deltas were mostly found in mandibular molars (n = 14, followed by maxillary incisors (n = 9. Only mandibular molars had interradicular canals. The detection of ramifications increased with the use of EDTA. However, no statistically significant relationship was found between the type of auxiliary chemical substance used and the number of root canal ramifications detected after root canal filling. It was concluded that the frequency of root canal ramifications found radiographically was low in treatments performed by undergraduate students.O objetivo deste estudo foi investigar radiograficamente a presença de ramificações do canal radicular encontrada depois do tratamento endodôntico, e determinar qualquer relação entre a presença destas e do tipo de substância química auxiliar usada. O estudo avaliou 1.470 tratamentos endodônticos executados pelos estudantes do último ano da Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (UNICAMP, SP, Brasil, no período de 1998 a

  5. Influência do ácido indolbutírico no enraizamento de alecrim-pimenta (Lippia sidoides em leito com umidade controlada Influence of indolebutyric acid on "alecrim-pimenta" (Lippia sidoides rooting bed under controlled humidity

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    L.S. Figueiredo

    2009-01-01

    Full Text Available A espécie Lippia sidoides (Verbenaceae é conhecida no Brasil como alecrim-pimenta, um arbusto típico da vegetação do nordeste brasileiro. Este trabalho foi realizado no Instituto de Ciências Agrárias da UFMG, em Montes Claros, Brasil, com a finalidade de estudar a propagação vegetativa do alecrim-pimenta em leito de enraizamento com umidade controlada. Estacas da planta foram submetidas às seguintes concentrações de ácido indolbutírico (IBA: 0, 250, 500, 750 e 1000 mg L-1 durante 24 horas. O experimento foi conduzido em delineamento inteiramente casualizado (DIC com cinco repetições, sendo dez estacas por repetição. Após 45 dias de implantação foram avaliados o número de raízes, o número de brotações, o comprimento da maior raiz, o índice de enraizamento e a porcentagem de enraizamento. Observou-se que as variáveis comprimento de raiz, número de raízes e índice de enraizamento apresentaram diferenças significativas entre as diversas concentrações de IBA. No entanto, a porcentagem de enraizamento não foi afetada significativamente, provavelmente pelo controle da umidade relativa e pela manutenção das folhas das estacas.The species Lippia sidoides Cham. (Verbenaceae is known in Brazil as "alecrim-pimenta", a typical shrub from the Northeastern Brazil vegetation. This work was carried out in the Agronomical Sciences Institute from Minas Gerais State University (UFMG, Montes Claros, Minas Gerais State, Brazil, in order to study the vegetative propagation of this species in rooting bed under controlled humidity. Herbaceous cuttings were treated with the following concentrations of indolebutyric acid (IBA: 0, 250, 500, 750, and 1000 mg L-1 for 24 hours. The experimental design was completely randomized (CRD with five replicates of 10 cuttings each. After 45 days, root and sprout numbers, the highest root length, and rooting index and percentage were evaluated. Root length and number and rooting index had

  6. Do ectomycorrhizal and arbuscular mycorrhizal temperate tree species systematically differ in root order based fine root morphology and biomass?

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    Petra eKubisch

    2015-02-01

    Full Text Available While most temperate broad-leaved tree species form ectomycorrhizal (EM symbioses, a few species have arbuscular mycorrhizas (AM. It is not known whether EM and AM tree species differ systematically with respect to fine root morphology, fine root system size and root functioning. In a species-rich temperate mixed forest, we studied the fine root morphology and biomass of three EM and three AM tree species from the genera Acer, Carpinus, Fagus, Fraxinus and Tilia searching for principal differences between EM and AM trees. We further assessed the evidence of convergence or divergence in root traits among the six co-occurring species. Eight fine root morphological and chemical traits were investigated in root segments of the first to fourth root order in three different soil depths and the relative importance of the factors root order, tree species and soil depth for root morphology was determined. Root order was more influential than tree species while soil depth had only a small effect on root morphology All six species showed similar decreases in specific root length and specific root area from the 1st to the 4th root order, while the species patterns differed considerably in root tissue density, root N concentration, and particularly with respect to root tip abundance. Most root morphological traits were not significantly different between EM and AM species (except for specific root area that was larger in AM species, indicating that mycorrhiza type is not a key factor influencing fine root morphology in these species. The order-based root analysis detected species differences more clearly than the simple analysis of bulked fine root mass. Despite convergence in important root traits among AM and EM species, even congeneric species may differ in certain fine root morphological traits. This suggests that, in general, species identity has a larger influence on fine root morphology than mycorrhiza type.

  7. Rooting induction of different Lavandula angustifolia accessions by auxin applicationIndução de enraizamento em diferentes acessos de Lavandula angustifolia por meio de aplicação de auxina

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    Claudine Maria de Bona

    2012-04-01

    Full Text Available Lavandula angustifolia Miller (lavender, considered a hard to root species, is comprised of many varieties and horticultural forms, and genotypes from different provenances may present variance in rooting capacity. The objective of this experiment was to test different concentrations of indol-3-butyric acid (IBA on rooting of eight (G1 to G8 L. angustifolia genotypes from different provenances. Cuttings were treated with different doses (0 to 2000 mg L-1 of IBA. Rooting percentage (RP, root number (RN, and length of the longest root (RL were evaluated. The different accessions presented very diverse response to IBA application. IBA did not exert influence on rooting of G1, G2, and G7; was benefic to G3 until 700 mg L-1; was benefic to G4 and G6; and was prejudicial to G5 and G8; the same IBA dose which promoted better rooting on G3 (700 mg L-1 was toxic to G5; 1500 mg L-1 of IBA promoted better rooting, greater root number and length on G6 but was completely and significantly detrimental to G8. G1 and G3 presented high rooting percentages, in spite of L. angustifolia being considered a hard to root species. G4, G5 and G6, however, presented very poor rooting, compared to the other genotypes. Necessity of auxin application and adequate dose may not be generalized to L. angustifolia different accessions. Its use should be incentivized when the genotype is recalcitrant to root and auxin concentration should be adjusted to it.A Lavandula angustifolia Miller (alfazema, considerada de difícil enraizamento, apresenta muitas variedades e formas horticulturais, e genótipos de diferentes procedências podem apresentar variação na sua capacidade de enraizamento. O objetivo deste experimento foi testar diferentes concentrações de ácido indol butírico (AIB no enraizamento de oito genótipos (G1 a G8 de L. angustifolia de diferentes procedências. Estacas foram tratadas com diferentes doses (0 a 2000 mg L-1 de AIB. Porcentagem de enraizamento (RN, n

  8. Larval control of Anopheles (Nyssorhinchus) darlingi using granular formulation of Bacillus sphaericus in abandoned gold-miners excavation pools in the Brazilian Amazon rainforest.

    Science.gov (United States)

    Galardo, Allan Kardec Ribeiro; Zimmerman, Robert; Galardo, Clícia Denis

    2013-01-01

    Use of a Bacillus sphaericus based mosquito larvicide was evaluated as an intervention for malaria vector control at a mining site in Amapá, Brazil. Impacts on larval and adult densities of the primary vector Anopheles darlingi were measured over the course of a 52 week study period. In Calçoene, State of Amapá, gold mining activity occurs in 19 mining sites in gold-miners of Lourenço. Large pools are formed in mining sites and naturally colonized by Anopheles darlingi. During one year, the impact of applications of VectoLex® CG to these larval sources was evaluated. Applications of 20kg/ha were made as needed, based on 10 immature (3rd, 4th instars and pupae) surveillance of health and established thresholds. One hundred percent initial control was observed 48h after each treatment. The pools received from 2-10 (5.3±1.6) treatments during the year. The average re-treatment interval in productive pools was 9.4±4.3 weeks. During weeks 3-52 of the study, mean density of late stage larvae was 78% and pupae were 93% lower in the treated pools than in untreated pools (p< 0.0001, n=51) while reduction of adult mosquitoes was 53% in comparison to the untreated area during the last five months of the study, which were the rainy season (p<0.001). VectoLex® CG reduced immature Anopheles darlingi infestation levels during the entire study period, and reduced adult mosquito populations during the rainy season.

  9. Larval control of Anopheles (Nyssorhinchus darlingi using granular formulation of Bacillus sphaericus in abandoned gold-miners excavation pools in the Brazilian Amazon Rainforest

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    Allan Kardec Ribeiro Galardo

    2013-04-01

    Full Text Available INTRODUCTION: Use of a Bacillus sphaericus based mosquito larvicide was evaluated as an intervention for malaria vector control at a mining site in Amapá, Brazil. Impacts on larval and adult densities of the primary vector Anopheles darlingi were measured over the course of a 52 week study period. METHODS: In Calçoene, State of Amapá, gold mining activity occurs in 19 mining sites in gold-miners of Lourenço. Large pools are formed in mining sites and naturally colonized by Anopheles darlingi. During one year, the impact of applications of VectoLex(r CG to these larval sources was evaluated. Applications of 20kg/ha were made as needed, based on 10 immature (3rd, 4th instars and pupae surveillance of health and established thresholds. RESULTS: One hundred percent initial control was observed 48h after each treatment. The pools received from 2-10 (5.3±1.6 treatments during the year. The average re-treatment interval in productive pools was 9.4±4.3 weeks. During weeks 3-52 of the study, mean density of late stage larvae was 78% and pupae were 93% lower in the treated pools than in untreated pools (p< 0.0001, n=51 while reduction of adult mosquitoes was 53% in comparison to the untreated area during the last five months of the study, which were the rainy season (p<0.001. CONCLUSIONS: VectoLex(r CG reduced immature Anopheles darlingi infestation levels during the entire study period, and reduced adult mosquito populations during the rainy season.

  10. Distribuição espacial do sistema radicular do cafeeiro fertirrigado por gotejamento em Campinas Root distribution of fertirrigated coffee trees in Campinas, State of São Paulo, Brazil

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    Carlos Vinícius Garcia Barreto

    2006-01-01

    Full Text Available Este estudo teve como objetivo avaliar o efeito da fertirrigação por gotejamento, utilizando-se emissores com diferentes espaçamentos (0,50 ou 0,80 m e profundidades de instalação (superficial, 0,10 e 0,20 m, na distribuição espacial do sistema radicular do cafeeiro. Observaram-se no cafeeiro irrigado e adubado de forma convencional diferentes condições de desenvolvimento radicular, variando conforme os tratamentos impostos. Para as plantas irrigadas por tubogotejadores com emissores espaçados a cada 0,50 m, a profundidade radicular efetiva foi menor (média de 0,63 m do que a observada para as plantas irrigadas por emissores posicionados a cada 0,80 m (média de 0,70 m. No manejo nutricional por fertirrigação observou-se menor desigualdade na profundidade radicular efetiva entre os tratamentos, bem como, em um aumento médio de 51,1% de densidade de raízes. Houve tendência de manutenção do volume radicular na região próxima aos emissores, enquanto nos pontos mais distantes do desenvolvimento do bulbo úmido, o crescimento radicular foi de 77%. A irrigação das plantas por tubogotejadores enterrados a 0,10 m de profundidade proporcionou maior desenvolvimento radicular em resposta à fertirrigação.This study aimed to evaluate the effect of the drip fertirrigation system, by using emitters with different arranging of spaces (0.50 or 0.80 m and depths of installation (superficial, 0.10 or 0.20 m on the ground, over the root spatial distribution of coffee tree. The coffee tree conventionally irrigated and fertilized presented different conditions of root development, varying according to the treatment imposed. For plants irrigated by emitters spaced every 0.50 m, the effective root depth was smaller (mean of 0.63 m than that observed for plants irrigated by emitters spaced every 0.80 m (mean of 0.70 m. The fertirrigation nutritional management allowed an uniform effective root depth among the treatments, as well as an increase

  11. Enraizamento de estacas de Chamaecyparis lawsoniana Parl. em cinco substratos com uso de ácido indolbutírico Rooting of Chamaecyparis lawsoniana Parl. cuttings with indolbutyric acid in five media

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    Elisabeth Regina Tempel Stumpf

    1999-06-01

    Full Text Available Com o objetivo de determinar o melhor substrato disponível na região para o enraizamento de estacas de Chamaecyparis lawsoniana Parl., utilizaram-se os substratos vermiculita fina, casca de arroz carbonizada, areia e as misturas casca de arroz carbonizada + vermiculita fina e areia + vermiculita fina (1:1 v/v. O experimento foi realizado em estufa do Departamento de Fitotecnia da Faculdade de Agronomia Eliseu Maciel/Universidade Federal de Pelotas, no período de maio a setembro de 1997, sob nebulização intermitente. Foram empregadas estacas apicais com 15cm de comprimento, submetidas ao tratamento com ácido indolbutírico (AIB na concentração de 10.000ppm. Foram efetuadas as seguintes avaliações: número de raízes por estaca, peso da matéria seca das raízes, comprimento da maior raiz por estaca e percentual de estacas enraizadas. Os resultados mostraram que dos substratos testados e com a concentração de AIB utilizada, a vermiculita fina apresentou os melhores resultados, com 99% das estacas enraizadas, maior número raízes por estaca (15, maior comprimento de raiz (12cm e maior peso da matéria seca das raízes (0,04g.To determine the best medium available in Pelotas, RS, for rooting of Chamaecyparis lawsoniana Parl. cuttings, the media fine vermiculite, carbonized rice hull, sand, carbonized rice hull + fine vermiculite and sand + fine vermiculite (1:1 v/v were used. This experiment was carried out in a glass greenhouse with intermitent mist at the Plant Science Department of Faculdade de Agronomia Eliseu Maciel/Universidade Federal de Pelotas, from May to September 1997. Apical cuttings 15cm long were treated with indolbutyric acid (IBA at 10.000ppm. The following evaluations were made: number of roots per cutting, weight of root dry matter, length of the longest root per cutting, and rooted cutting percentage. The results show that the vermiculite medium, with the IBA concentration utilized, is the most suitable for the

  12. Alterações anatômicas em folhas e raízes de Jatropha curcas L. cultivadas sob estresse salino Anatomical changes in the leaves and roots of Jatropha curcas L. cultivated under saline stress

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    Gemima Manço de Melo

    2011-09-01

    Full Text Available A espécie Jatropha curcas L. está entre as mais destacadas fontes de grãos oleaginosos, com baixa exigência hídrica e nutricional, sendo promissora para regiões áridas e semiáridas, em geral sujeitas à salinização do solo. Objetivou-se neste trabalho avaliar o efeito de diferentes concentrações salinas, sobre a anatomia de folhas e raízes de Jatropha curcas. Cinqüenta sementes foram semeadas em bandejas, e as plântulas transferidas para potes com capacidade para 5 litros, o ensaio foi conduzido em casa de vegetação. Após 21 dias da germinação, as plantas foram submetidas aos seguintes tratamentos salinos: 25; 50; 75; 100; 150 e 200 mM de NaCl, além do controle (0 mM de NaCl, em delineamento inteiramente casualizado, com cinco repetições por tratamento. Ao final do 32º dia de tratamento, a terceira folha e raízes, medindo aproximadamente 5 cm de comprimento, foram coletadas e fixadas em FAA 50. Seções transversais da porção mediana da nervura central da folha e da região mediana da raiz foram cortadas e coradas com safrablau. Foram observadas na folha redução no número de células do xilema e floema e alterações em sua distribuição, em função do aumento das concentrações de sal na solução. Nas raízes observou-se que, quanto mais alta a concentração salina, maior a lignificação das células xilemáticas e endodérmicas, e maior a redução no diâmetro e no número de elementos de vaso. Portanto, ocorrem alterações anatômicas em folhas e raízes das plantas quando cultivadas sob altas concentrações salinas.The species Jatropha curcas L. is among the most prominent sources of oilseeds, with low water requirement and nutrition, and promising to arid and semiarid regions, usually subject to soil salinization. The objective of this study was to evaluate the effect of different salt concentrations on the anatomy of leaves and roots of Jatropha curcas. Fifty seeds were sown in trays and the seedlings

  13. Do ectomycorrhizal and arbuscular mycorrhizal temperate tree species systematically differ in root order-related fine root morphology and biomass?

    OpenAIRE

    Kubisch, Petra; Hertel, Dietrich; Leuschner, Christoph

    2015-01-01

    While most temperate broad-leaved tree species form ectomycorrhizal (EM) symbioses, a few species have arbuscular mycorrhizas (AM). It is not known whether EM and AM tree species differ systematically with respect to fine root morphology, fine root system size and root functioning. In a species-rich temperate mixed forest, we studied the fine root morphology and biomass of three EM and three AM tree species from the genera Acer, Carpinus, Fagus, Fraxinus, and Tilia searching for principal dif...

  14. Distribuição radicular de cultivares de aspargo em áreas irrigadas de Petrolina - PE Root distribution of asparagus cultivars in irrigated areas of Petrolina, Brazil

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    Luís Henrique Bassoi

    2001-03-01

    Full Text Available A distribuição radicular de duas cultivares de aspargo (New Jersey 220 e UC 157 F1, irrigadas por aspersão convencional, foi avaliada durante o ano de 1997 em solos de textura arenosa, em plantio experimental e comercial, respectivamente, nos Projetos de Irrigação de Bebedouro e Senador Nilo Coelho, em Petrolina (PE. O objetivo foi obter informações do sistema radicular do aspargo, empregando-se os métodos do monolito e do perfil de solo auxiliado pela análise de imagens digitais, para o manejo de solo e água nesse cultivo. Na área experimental, a maior parte da matéria seca, área e comprimento de raízes no perfil de solo e densidade de comprimento radicular foram encontradas até a profundidade de 0,4 m nas duas cultivares, enquanto que na comercial a maior parte da área e comprimento de raízes no perfil do solo estendeu-se até a profundidade de 0,6 m (cv. New Jersey 220. Nesses dois plantios, as raízes das cultivares atingiram a profundidade de 1 m. Na área experimental, a massa seca, a área e o comprimento no perfil de solo, e a densidade de comprimento radicular nas cultivares concentraram-se até a distância de 0,6 m à linha de plantas. No intervalo de diâmetro (d de raízes 2In 1997 the root distribution of two asparagus cultivars (New Jersey 220 and UC 157 F1 irrigated by sprinkler was evaluated in coarse textured soils in experimental and commercial areas at Petrolina county, in the semi-arid region of northeastern Brazil, to obtain useful information for soil and water management. Both the monolith and the soil profile aided by digital image analysis methods were used to evaluate it. In the experimental area, a greater concentration of root dry weight, root area and length in the soil profile and root length density were found up to 04 m depth for both cultivars, while in commercial area the root area and length of cv. New Jersey 220 were concentrated until 0,6 m depth. Roots reached 1 m depth in both areas. In

  15. Nematóide de galha em rabanete: suscetibilidade de cultivares e patogenicidade Root-knot nematode: cultivars reaction and damage to radish

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    Carlos Eduardo Rossi

    2004-03-01

    Full Text Available Pesquisaram-se as reações de 11 cultivares de rabanete a Meloidogyne javanica e sua patogenicidade a uma dessas cultivares. No estudo de reações, os tratamentos/cultivares foram 'Comprido Branco', 'Crimson Gigante', 'Ponta Branca', 'Comprido Vermelho', 'Serrano', 'Gigante Wurzburgo', 'Saxa', 'Redondo Vermelho', 'Redondo Gigante', 'Cometo' e 'Akamaru Hatsuka'. Determinaram-se os índices de galhas (IG e de massas de ovos (IMO, o número de nematóides no sistema radicular (NSR e por grama de raiz (NGR e o fator de reprodução (FR 53 dias após a inoculação artificial com 2.000 ovos do parasito. Em outro experimento, avaliaram-se os efeitos de três densidades populacionais do nematóide [0, 500 (nível populacional baixo e 10.000 (nível populacional alto ovos/planta] sobre as massas frescas e secas de túberas e de parte aérea da cultivar 'Redondo Gigante', 39 dias após a inoculação. Os resultados mostraram que todas as cultivares permitiram a reprodução de M. javanica, sendo portanto consideradas suscetíveis. Valores de IG e IMO foram maiores ou iguais a 2,5 e os de FR, maiores do que 8,0 para todas as cultivares estudadas. O parasito causou diminuição significativa nas massas frescas e secas de túberas e de partes aéreas nos dois níveis populacionais estudados comparados com o controle não inoculado. As médias dos tratamentos contendo níveis populacionais baixo e alto do nematóide também diferiram estatisticamente entre si, comprovando-se, assim, a sua ação patogênica sobre a cultivar avaliada.Experiments were conducted under greenhouse conditions to determine the reaction of eleven radish (Raphanus sativus cultivars to Meloidogyne javanica and the pathogenicity of this nematode to a previously selected cultivar. The cultivars tested were 'Comprido Branco', 'Crimson Gigante', 'Ponta Branca', 'Comprido Vermelho', 'Serrano', 'Gigante Wurzburgo', 'Saxa', 'Redondo Vermelho', 'Redondo Gigante', 'Cometo' and 'Akamaru

  16. Evaluation of larvicidal activity of the methanolic extracts of Piper alatabaccum branches and P. tuberculatum leaves and compounds isolated against Anopheles darlingi

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    Frances T. T. Trindade

    2012-10-01

    Full Text Available Piper is a notable genus among Piperaceae due to their secondary metabolites such as lignans, amides, esters and long chain fatty acids used as anti-herbivore defenses with comparable effects of pyrethroids, that holds a promise in insect control, including malaria vectors such as Anopheles darlingi, the main vector in the North of Brazil. Methanolic extracts of Piper tuberculatum Jacq., Piperaceae, and P. alatabaccum Trel. & Yunck., Piperaceae, and some isolated compounds, i.e, 3,4,5-trimetoxy-dihydrocinamic acid, dihydropiplartine; piplartine, piplartine-dihydropiplartine and 5,5',7-trimetoxy-3',4'-metilenodioxiflavone were tested as larvicides against A. darlingi. The Lethal Concentrations (LC50 and LC90 of methanolic extracts were 194 and 333 ppm for P. tuberculatum and 235 and 401 ppm for P. alatabacum, respectively. Isolated compounds had lower LC values, e.g. the LC50 and LC90 of the piplartine-dihidropiplartine isolated from both plant species was 40 and 79 ppm, respectively.

  17. Prevalence of antibodies to Trypanosoma cruzi, Toxoplasma gondii, Encephalitozoon cuniculi, Sarcocystis neurona, Besnoitia darlingi, and Neospora caninum in North American opossums, Didelphis virginiana, from southern Louisiana.

    Science.gov (United States)

    Houk, Alice E; Goodwin, David G; Zajac, Anne M; Barr, Stephen C; Dubey, J P; Lindsay, David S

    2010-12-01

    We examined the prevalence of antibodies to zoonotic protozoan parasites ( Trypanosoma cruzi, Toxoplasma gondii, and Encephalitozoon cuniculi) and protozoans of veterinary importance ( Neospora caninum, Sarcocystis neurona, and Besnoitia darlingi) in a population of North American opossums ( Didelphis virginiana) from Louisiana. Samples from 30 opossums were collected as part of a survey for T. cruzi in Louisiana. Frozen sera from these 30 opossums were examined using an indirect immunofluorescent antibody test (IFAT) against in vitro-produced antigenic stages of these protozoans. Additionally, 24 of the 30 samples were examined using hemoculture, and all 30 were examined in the modified direct agglutination test (MAT) for antibodies to To. gondii. The prevalences of reactive IFAT samples were as follows: 60% for T. cruzi, 27% for To. gondii, 23% for E. cuniculi, 17% for S. neurona, 47% for B. darlingi, and 0% for N. caninum. Hemoculture revealed that 16 (67%) of 24 samples were positive for T. cruzi, compared to 18 of 30 (60%) by IFAT. The sensitivity and specificity for the IFAT compared to hemoculture was 100% for each. The modified direct agglutination test revealed that 9 (30%) of the 30 samples from opossums had antibodies to To. gondii , compared to 8 (27%) using the IFAT. The sensitivity and specificity of the IFAT compared to the MAT was 100% and 72%, respectively.

  18. Arbuscular mycorrhizal fungi and the occurrence of flavonoids in roots of passion fruit seedlings Fungos micorrízicos arbusculares e a ocorrência de flavonóides em raízes de mudas de maracujazeiro amarelo

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    Ana Cristina Fermino Soares

    2005-08-01

    Full Text Available Composition and the role of root flavonoids in the regulation of mycorrhizal symbiosis are still poorly understood. Several flavonoids stimulate spore germination, mycelia growth and root colonization by arbuscular mycorrhizal fungi (AMF, and both root colonization and flavonoid composition are affected by plant nutritional status. Effects of AMF on the occurrence and content of aromatic secondary metabolites in the roots of passion fruit seedlings grown under two levels of phosphorus (P fertilization (10 and 50 mg kg-1 of phosphorus was studied. Seedlings were inoculated with Glomus clarum and a population of native fungi from a passion fruit plantation. Methanolic extracts of passion fruit seedlings roots were analyzed by high performance liquid chromatography (HPLC. It was recorded the occurrence of several compounds, possibly flavonoids, with seven major peaks. The root contents of the compound with a retention time of 4.5 minutes, varied in response to the root colonization by different mycorrhizal fungi, and the contents of two compounds with retention times of 3.4 and 18.9 minutes varied due to the poor plant growth and nutritional status. Passion fruit seedlings have several aromatic compounds, and their contents were correlated with root colonization by different mycorrhizal fungi, the reduced seedling growth due to nutritional stress, and/or the plant defense responses to the fungi.Os flavonóides nas raízes e seu papel na regulação da simbiose com fungos micorrízicos não são bem conhecidos. Vários flavonóides estimulam a germinação de esporos, crescimento micelial e colonização micorrízica. Ambos, a colonização micorrízica e a composição de flavonóides nas raízes são afetados pelo estado nutricional da planta. Avaliou-se o efeito de fungos micorrízicos arbusculares sobre a ocorrência e concentração de substâncias, possivelmente metabólitos aromáticos secundários pertencentes à classe dos flavonóides, em

  19. Annual variations in the number of malaria cases related to two different patterns of Anopheles darlingi transmission potential in the Maroni area of French Guiana

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    Issaly Jean

    2010-03-01

    Full Text Available Abstract Background With an Annual Parasite Incidence (API of 132.1, in the high and moderate risks zones, the Maroni area of French Guiana has the second highest malaria incidence of South-America after Guyana (API = 183.54 and far above Brazil (API = 28.25. Malaria transmission is occurring despite strong medical assistance and active vector control, based on general WHO recommendations. This situation is generated by two main factors that are the social and cultural characteristics of this border area, where several ethnic groups are living, and the lack of understanding of transmission dynamics of the main mosquito vector, Anopheles darlingi. In this context, entomological data collected in two villages belonging to two different ethnic groups of the French border of the Maroni River, were retrospectively analysed to find out how the mosquito bionomics are related to the malaria transmission patterns. Methods Data were provided by human landing catches of mosquitoes carried out each month for two years in two villages belonging to two ethnic groups, the Amerindians Wayanas and the Aloukous of African origin. The mosquitoes were sorted by species, sex, date, hour and place of collection and processed for Plasmodium sp. parasite detection. The data were compiled to provide the following variables: human biting rates (HBR, parity rates (PR, numbers of infective bites (IB, entomological inoculation rates (EIR and numbers of infected mosquitoes surviving enough to transmit (IMT. Spatial and temporal differences of variables between locations and during the night were tested by the Kruskall-Wallis analysis of variance to find out significant variations. Results The populations of the main mosquito vector An. darlingi showed significant variations in the spatial and temporal HBR/person/night and HBR/person/hour, IB/person/month and IB/person/hour, and IMT/village/night and IMT/village/hour. In the village of Loca (Aloukous, the IMT peaked from June

  20. EMERGÊNCIA E CRESCIMENTO DE CAGAITA (Eugenia dysenterica DC. EM FUNÇÃO DO TIPO E DO VOLUME DE SUBSTRATOS EMERGENCE AND GROWTH OF CAGAITA (Eugenia dysenterica DC AS INFLUENCED BY TYPE AND VOLUME OF ROOTING MEDIA

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    Eli Regina Barboza de Souza

    2007-09-01

    of substract in tube-like plastic containers with 50 cm3, 120 cm3 and 228 cm3, and three types of rooting media such as soil + forest humus + vermiculite (1:1:2, the same with 1 gram of Yoorin (thermophosphate per liter, and industrial organic compound Plantmax. The experiment was arranged in randomized complete block design with eight replications and split plots with 16 containers each. The rooting medium soil + forest humus + vermiculite showed to be favorable for fresh and dry matter production of over ground parts and underground parts of the plants. A good response was also obtained with the fertilizer treatment. The best growth of plants was observed in the bigger containers. Nevertheless, 160 days after seeding the transplants had not reached the stem diameter sufficient for grafting.

    KEY-WORDS: Cerrado fruit tree; <em>Myrtaceae>; propagation.

  1. Implante de tubo valvulado bioprótese "stentless" em posição aórtica: estudo experimental em ovinos A juvenile sheep model for the stentless bioprostheses implanted as aortic root replacements

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    Paulo César SANTOS

    2002-03-01

    Full Text Available INTRODUÇÃO: O tratamento das lesões valvares representa um grande desafio dentro da cirurgia cardiovascular, pois, até os dias atuais, não temos um substituto ideal para as valvas do coração. As próteses atualmente disponíveis apresentam vantagens e desvantagens; as mecânicas exigem anticoagulação definitiva e as biológicas apresentam baixa durabilidade. Para se chegar a um substituto ideal, são necessárias várias fases, desde a escolha e o preparo do material até o implante em animal de grande porte. Esta última é muito importante, pois a escolha do animal deve ser criteriosa. Ovinos juvenís são animais dóceis de fácil manipulação e suas características anatômicas e biológicas se assemelham muito ao que ocorre com o ser humano e as próteses "stentless" são substitutos mais próximos dos homoenxertos. MATERIAL E MÉTODO: Foram operados 30 ovinos juvenís com idade variando de 4,4 a 6,3 meses, peso de 25 a 37 kg, sendo 28 machos. Todos os animais foram submetidos a circulação extracorpórea para implante de bioprótese "stentless" montada em tubo de aorta porcina, com reimplante de artérias coronárias. A operação foi realizada com hipotermia moderada e cardioplegia gelada. O período de seguimento foi de 30 dias e, ao final, realizado ecocardiograma. RESULTADOS: Houve 8 (26,6% óbitos no intra-operatório e 6 (27,7% óbitos no seguimento. O tempo médio de CEC foi de 98 min. Foi realizado ecocardiograma em 16 animais e em nenhum caso foram vistos sinais de endocardite ou outras alterações; a fração média de ejeção foi de 74%. CONCLUSÃO: Este modelo experimental utilizando ovinos para o estudo de biopróteses "stentless" em posição aórtica mostrou-se bem reprodutível e a performance do enxerto bastante factível de ser avaliada, pois ovinos são animais dóceis e de fácil manipulação no seguimento tardio.INTRODUCTION: Often, studies on aortic prosthetic valves analyze their performance not at the

  2. Incidência de injúrias mecânicas em raízes de mandioquinha-salsa na cadeia de pós-colheita Incidence of mechanical injury in arracacha roots at the postharvest handling system

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    Roberto Marques Souza

    2003-12-01

    Full Text Available O tamanho e a aparência das raízes de mandioquinha-salsa são os principais atributos de qualidade na comercialização, e os danos mecânicos diminuem sua durabilidade e valor como mercadoria. O objetivo deste trabalho foi caracterizar e avaliar a incidência de injúrias mecânicas em raízes de mandioquinha-salsa em cinco etapas do manuseio pós-colheita, dividido em colheita, beneficiamento (antes e após a lavação, atacado e varejo. A etapa da colheita foi avaliada em Castro(PR, o beneficiamento nas lavouras de Tapiraí(SP, atacado na CEAGESP(SP e o varejo em supermercados da grande São Paulo. Em cada etapa foram selecionadas ao acaso 110 raízes, em dez amostragens (repetições em locais e épocas diferentes, entre março/2000 e junho/2001. As injúrias mecânicas foram categorizadas em quatro tipos principais: abrasão, ruptura parcial, rachadura e quebra das raízes. Como as lesões superficiais causadas pela abrasão apresentaram grande variação na extensão dos danos, foi elaborada uma escala de cinco notas (1=sem lesão; 5=>40% superfície lesionada para facilitar a avaliação. As lesões superficiais causadas por abrasão foram o tipo de injúria mecânica com maior incidência em todas as etapas do manuseio, sendo 13,3% no produtor, 19,7% nas raízes sem lavar, 24,9% nas raízes lavadas, 47,2% no atacado, chegando a 78,9% no varejo. O atacado foi a etapa em que constatou-se a maior incidência de outros tipos de injúrias (ruptura, 7,6%; rachadura, 4,2%; quebra, 10,6%. O ponto crítico para a incidência de injúrias mecânicas ocorreu entre as etapas beneficiamento-atacadista, provavelmente devido à embalagem (caixa de madeira do tipo "K" e ao transporte inadequados.Size and appearance are the most important quality characteristics of arracacha roots. The aim of this experiment was to determine the main types and the incidence of mechanical injuries in arracacha roots and evaluate its incidence at the different stages of

  3. Evaluation of apical deviation in root canals instrumented with K3 and ProTaper systems Avaliação do desvio apical em canais instrumentados pelos sistemas K3 e ProTaper

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    Mariana Diniz Bisi dos Santos

    2006-12-01

    Full Text Available OBJECTIVES: this study evaluated the apical deviation of curved root canals instrumented with K3 and ProTaper systems. MATERIAL AND METHODS: twenty root canals of human maxillary and mandibular first molars were employed, which were divided into 2 groups: group A (10 teeth was instrumented with the K3 system, and group B (10 teeth with the ProTaper system. Evaluation of deviation was performed by double radiographic exposure. Radiographs were achieved before and after instrumentation, with 0.3-second, thus allowing superimposition of images. Three-dimensional computerized tomograph was performed in 3 specimens in each group, as an additional means to evaluate the apical deviation. RESULTS: were evaluated by the parametric test Student-Newman-Keuls at 5%, which did not reveal significance between groups concerning the apical deviation. The results of computerized tomograph images demonstrated that the larger deviation of the root canal occurred at the distolingual area for both systems. CONCLUSIONS: both techniques produced a mild apical deviation. Computerized microtomography was shown to be accurate for experimental endodontics studies.OBJETIVOS: avaliar o desvio apical de canais radiculares curvos instrumentados pelos sistemas K3 e ProTaper. MATERIAIS E MÉTODOS: foram utilizados 20 canais radiculares de primeiros molares superiores e inferiores humanos, os quais foram divididos em 2 grupos, o grupo A (10 dentes foi instrumentado pelo sistema K3 e o grupo B (10 dentes com o sistema ProTaper, a forma de avaliação do desvio foi a dupla exposição radiográfica. As tomadas radiográficas foram feitas antes e após a instrumentação, com um tempo de 0,3 segundos em cada exposição, ocorrendo assim, sobreposição das imagens. Em 3 espécimes, de cada grupo, foi realizada a tomografia computadorizada tridimensional, como forma adicional de avaliar o desvio apical . Os resultados obtidos foram avaliados pelo teste paramétrico Student

  4. Root fractures

    DEFF Research Database (Denmark)

    Andreasen, Jens Ove; Christensen, Søren Steno Ahrensburg; Tsilingaridis, Georgios

    2012-01-01

    The purpose of this study was to analyze tooth loss after root fractures and to assess the influence of the type of healing and the location of the root fracture. Furthermore, the actual cause of tooth loss was analyzed.......The purpose of this study was to analyze tooth loss after root fractures and to assess the influence of the type of healing and the location of the root fracture. Furthermore, the actual cause of tooth loss was analyzed....

  5. Sistema radicular e nutrição da soja em função da compactação do solo Soybean nutrition and root growth as affected by soil compaction

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    Ciro Antonio Rosolem

    1994-01-01

    Full Text Available A compactação do solo diminui o crescimento radicular, podendo afetar tanto o desenvolvimento quanto a produtividade da soja. No presente trabalho, estudaram-se os efeitos da compactação subsuperficial na morfologia radicular da soja (Glycine max L. Merrill, procurando relacioná-los ao crescimento e à nutrição da planta. O 'Primavera' foi cultivado até os 37 dias da emergência, em vasos onde a camada de 15-18,5 cm de profundidade foi campactada a 1,03, 1,25, 1,48 e 1,72 g/cm³, em um latossolo vermelho-escuro com 80% de areia e 16% de argila e cuja compactação em subsuperfície levou a um acúmulo de raízes na camada superficial do vaso, sem grandes conseqüências na nutrição da planta. Na densidade aparente de 1,72 g/cm3, as raízes não conseguiram penetrar, embora já houvesse alguma restrição ao crescimento na densidade de 1,25 g/cm³. Quando a camada compactada apresentava resistência à penetração de 0,69 MPa, houve uma redução de 50% no crescimento radicular da soja.Soil tillage can originate compacted layers in the soil subsurface. This compaction reduces root growth, plant development and eventually soybean yields. This study examined the effects of subsurface compaction on soybean root growth and morphology as related to plant growth and mineral nutrition. Soybean plants cv. Primavera were grown up to 37 days from seedling emergence in pots where a compacted layer was set at the 15.0 to 18.5 cm depth. This layer was compacted to bulk densities of 1.03, 1.25, 1.48 and 1.72 g/cm³, corresponding to cone penetrometer resistances of 0.05, 3.0, 7.5 and 2.0 MPa, respectively. The soil was a Dark Red Latosol (Haplortox, sandy loam, with 80% of sand and 16% of clay. Soil subsurface compaction led to a higher concentration of roots in the surface layer of the pots, without significant effects on plant canopy growth and nutrition. In the presence of a compacted layer the roots were shorter, but nutrient absorption/cm of

  6. Avaliação do enraizamento, desenvolvimento de raízes e parte aérea de porta-enxertos de videira em condições de campo Evaluation of rooting, development of roots and shoot biomass from rootstock of grapevine, in field conditions

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    Marco Antonio Tecchio

    2007-12-01

    Full Text Available Objetivou-se neste trabalho avaliar o enraizamento, a brotação e o desenvolvimento de raízes de diferentes porta-enxertos de videira em condições de campo. Estacas lenhosas dos porta-enxertos '420 A', 'Golia', '5C', '8B', 'RR101-14', 'SO4', '99R', 'Kober 5BB', 'IAC 766', 'IAC 572', 'IAC 571-6', 'Ripária do Traviú' e 'Rupestris du Lot' foram colocadas em canteiro de terra, sem tratamento prévio. O delineamento foi em blocos ao acaso, com cinco repetições e vinte estacas por parcela, com as estacas dispostas em espaçamento de 12 x 5cm. As estacas foram colocadas para enraizar no início de julho e removidas no final de setembro para as avaliações. A porcentagem de estacas enraizadas variou de 79% para 'Ripária do Traviú' a 99% para o 'RR101-14'. Quanto à brotação, o 'Ripária do Traviú' apresentou 47%, 'IAC 571-6', 'IAC 572', '420 A', 'Rupestris du Lot', 'Kober', 'IAC 766', '8B', '5C', apresentaram de 76 a 89% e 'Golia', 'SO4', '99R' e 'RR 101-14' mais de 90%. 'IAC 572' e 'IAC 571-6' apresentaram o menor número de raízes por estaca, no entanto, foram as que apresentaram raízes mais desenvolvidas, seguidas pelo '5C' e 'Rupestris du Lot'. 'Kober 5BB' e 'Ripária do Traviú' apresentaram as raízes menos desenvolvidas. As demais variedades apresentaram valores intermediários. Concluiu-se que, entre todos os porta-enxertos, o 'Ripária do Traviú' apresentou os menores índices de enraizamento e brotação das estacas, nas condições de campo.The goals of this investigation was to evaluate the rooting, budding and development of roots from different rootstock of grapevine, in field conditions. Ligneous cutting of rootstock '420 A', 'Golia', '5C', '8B', 'RR101-14', 'SO4', '99R', 'Kober 5BB', 'IAC 766', 'IAC 572', 'IAC 571-6', 'Ripária do Traviú' and 'Rupestris du Lot' were planted in soil, without previous preparation. The experimental design was done in randomized blocks, with five repetitions and twenty cutting per plot

  7. Atributos químicos e perfil de enraizamento de milho influenciados pela calagem em semeadura direta Chemical attributes and corn root system development as affected by liming in no-till

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    Gabriel Henrique Melo Nascimento Leite

    2006-08-01

    ,20-0,30 m. Mesmo sob condições químicas adversas, em todos os tratamentos, o milho apresentou crescimento de raízes até 0,45 m de profundidade. O tratamento 100I apresentou maior crescimento de raízes até 0,15-0,20 m de profundidade do que os tratamentos com doses menores de calcário em superfície (50S e 25S, onde as raízes se concentraram na camada até 0,075 m. A incorporação do calcário proporcionou maior uniformidade na neutralização da acidez do solo em profundidade, o que se refletiu em maior quantidade de raízes até 0,45 m.In Rio Grande do Sul state, the area cultivated with under no-till system now amounts to 60 % of the total rain-fed cultivated area. However, there are still doubts as to crop practices in this soil conservation system, such as liming and fertilization as well as regarding root growth and crop productivity. The objective of this study was to evaluate the effects of lime doses and application forms on corn root development. The experiment was carried out in 1999/2000, in the experimental area of the Soil Science Department of the Federal University of Santa Maria, state of Rio Grande do Sul, Brazil, in a random block design in split plots, with four replications. The soil under study was a typical Hapludalf with 2 % slope. Main treatments were: no lime (SC, incorporation of 100 % of the recommended dose (100I, and 25 % (25S, 50 % (50S and 100 % (100S of the recommended dose (6.8 t ha-1 distributed over the soil surface. Lime was incorporated with conventional tillage in February 1996. Using the "nail board" method, with dimensions of 0,20 x 0,30 m, 0,30 x 0,45 m and 0,45 x 0,70 m, monoliths were taken from the soil profile, parallel to the corn sowing furrow, at 20, 54 and 80 days after corn emergence (DAE. The monoliths were carefully washed and the roots counted to establish a corn rooting profile in each treatment. Increasing lime doses applied on the soil surface (25S, 50S and 100S increased the depth at which elevations in p

  8. Vida útil e metabolismo de carboidratos em raízes de mandioquinha-salsa sob refrigeração e filme de PVC Shelf life and carbohydrate metabolism of arracacha roots stored under refrigeration and PVC film

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    Rosilene Antonio Ribeiro

    2007-04-01

    Full Text Available O objetivo deste trabalho foi avaliar a influência da temperatura de armazenamento e do uso do filme de cloreto de polivinila (PVC sobre a perda de matéria fresca e água, incidência de danos causados por frio e metabolismo pós-colheita dos carboidratos, em raízes tuberosas de mandioquinha-salsa (Arracacia xanthorrhiza. O filme de PVC reduziu a perda de matéria fresca e manteve o teor de água das raízes, durante o armazenamento por 60 dias a 5 e 10ºC. Os danos causados por frio foram inibidos nas raízes embaladas em filme de PCV, em ambas as temperaturas de armazenamento. As baixas temperaturas induziram o acúmulo de açúcares solúveis e a degradação de amido e, para as raízes armazenadas sem PVC, o aumento do conteúdo dos açúcares solúveis foi transiente e a taxa de degradação de amido foi superior à das raízes armazenadas com PVC.The objective of this work was to evaluate the influence of the storage temperature and stretch polyvinylchloride (PVC film on the loss of fresh weight and water, on the development of chilling injury symptoms, and on the postharvest metabolism of carbohydrates, in arracacha tuber roots (Arracacia xanthorrhiza. The PVC film reduced the fresh weight loss and kept water content in the roots during 60-day storage period at 5 and 10ºC. PVC film in both storage temperatures inhibited the development of external and internal chilling injury symptoms. The low temperatures induced the increase of soluble sugar content and decrease of starch concentration, where the increase in soluble sugar was transient in roots stored without PVC film, and the rate of starch degradation was higher compared to the roots stored with PVC.

  9. Root Hairs

    Science.gov (United States)

    Grierson, Claire; Nielsen, Erik; Ketelaarc, Tijs; Schiefelbein, John

    2014-01-01

    Roots hairs are cylindrical extensions of root epidermal cells that are important for acquisition of nutrients, microbe interactions, and plant anchorage. The molecular mechanisms involved in the specification, differentiation, and physiology of root hairs in Arabidopsis are reviewed here. Root hair specification in Arabidopsis is determined by position-dependent signaling and molecular feedback loops causing differential accumulation of a WD-bHLH-Myb transcriptional complex. The initiation of root hairs is dependent on the RHD6 bHLH gene family and auxin to define the site of outgrowth. Root hair elongation relies on polarized cell expansion at the growing tip, which involves multiple integrated processes including cell secretion, endomembrane trafficking, cytoskeletal organization, and cell wall modifications. The study of root hair biology in Arabidopsis has provided a model cell type for insights into many aspects of plant development and cell biology. PMID:24982600

  10. Soil water extraction by roots and Kc for the coffee crop Extração radicular de água e Kc em cultura de café

    Directory of Open Access Journals (Sweden)

    Adriana L. da Silva

    2009-06-01

    Full Text Available Basic information for a rational soil-water management of the coffee crop is still insufficient, particularly under irrigated conditions. Of great importance for the estimation of water requirements of coffee crops are their root distribuition and evapotranspiration crop coefficients. This study compares soil water extraction by roots of coffee plants of the variety "Catuaí Vermelho" (IAC-44, grown in Piracicaba, SP, Brazil, 3 to 5 years old, with direct measurements of root dry matter, showing a good agreement between both approaches, and confirming that most of the root system is distributed in the top soil layer (0-0.3 m and that less than 10% of the root system reaches depths greater than 1.0 m. Calculated evapotranspiration crop coefficients are in agreement with those found in the literature, with an average of 1.1, independent of shoot dry matter, plant height and leaf area index.A informação básica para o manejo racional de água e solo na cultura de café ainda é insuficiente, sobretudo em condições de cultura irrigada. A distribuição radicular e os coeficientes de cultura para evapotranspiração são de grande importância para a estimativa da necessidade de água na cultura café. Este estudo compara a extração radicular de água de plantas de café, variedade "Catuaí Vermelho" (IAC-44, cultivado em Piracicaba, SP, Brasil, de 3 a 5 anos de idade, com medidas diretas de material seca de raiz, mostrando boa relação entre as metodologias e confirmando que a maioria do sistema radicular está distribuída na camada superficial de solo (0-0,3 m e que menos de 10% do sistema radicular atingem profundidades maiores que 1,0 m. Os coeficientes de cultura medidos estão de acordo com aqueles encontrados na literatura, com média de 1,1, independentemente da matéria seca, altura de planta e índice de área foliar.

  11. Qualidade de raízes de cenoura em sistemas consorciados com alface sob diferentes densidades populacionais Quality of carrot roots in intercropped systems with lettuce under different planting densities

    Directory of Open Access Journals (Sweden)

    Aurélio P. Barros Júnior

    2005-06-01

    Full Text Available O experimento foi realizado de junho a setembro de 2003, em campo da ESAM, com o objetivo de avaliar a qualidade de raízes de cenoura (Brasília em sistemas consorciados com alface (Tainá sob diferentes densidades populacionais. O delineamento experimental utilizado foi de blocos casualizados, em esquema fatorial 4 x 4, com três repetições. Os tratamentos foram resultantes da combinação de quatro populações de cenoura [(40%, 60%, 80% e 100% da população recomendada no cultivo solteiro (PRCS] com quatro populações de alface (40%, 60%, 80% e 100% da PRCS. As características avaliadas nas raízes da cenoura foram acidez total titulável (ATT, sólidos solúveis totais (SST, açúcares totais (ACT pH, relação sólidos solúveis totais e acidez total titulável, além da produtividade comercial da cenoura e da alface. Ocorreu interação significativa entre as densidades populacionais das culturas componentes na ATT das raízes da cenoura. Houve aumento no conteúdo de SST e no pH à medida que se aumentou a densidade populacional de cenoura. Houve também um aumento na produtividade comercial de raízes de cenoura e na produtividade da alface com o aumento de suas densidades populacionais, respectivamente. A variação na população de cenoura não afetou a produtividade da alface, mas a variação na população da alface afetou negativamente a produtividade comercial da cenoura. Entre as características avaliadas, as que se correlacionaram de maneira significativa com a produtividade comercial de cenoura, foram as concentrações de sólidos solúveis totais e açucares totais, evidenciando assim, que estes atributos podem ser considerados representativos da qualidade de raízes da cenoura.The experiment was carried out from June to September 2003, in Mossoró, Rio Grande do Norte State, Brazil, to evaluate the quality of carrot roots (cv. Brasília in intercropped systems with lettuce (cv. Tainá under different planting

  12. Technical innovation in Marx and Mill and the roots of contemporary ambientalist thought A inovação técnica em Mill e Marx e as raízes do pensamento preservacionista contemporâneo

    Directory of Open Access Journals (Sweden)

    Marcia Regina Gabardo da Camara

    1994-08-01

    Full Text Available This article discusses the views of Mill and Marx on technical innovation and ambiental preservations necessities. Although innovation brings wealth to some people and some nations; the heritage to the population is the depredation and degradation of earth -land and men. These are the roots of contemporary ambientalist thought. O artigo apresenta a visão dos economistas clássicos ingleses Mill e Marx acerca da inovação técnica e destaca que o progresso técnico não deve causar a destruição do meio ambiente em sentido amplo -terra e homem. O caráter inédito do artigo é destacar que embora estivessem em pólos opostos os autores concordaram que os frutos do progresso técnico não foram igualmente distribuídos e o patrim6nio ambiental na fase inicial da revolução industrial foi dilapidado, quando poderiam ter conduzido a um mundo melhor as populações miseráveis dos países desenvolvidos e em desenvolvimento. Os dois autores se constituem nas matrizes do pensamento preservacionista contemporâneo.

  13. In vitro evaluation of the effectiveness of the chemomechanical preparation against Enterococcus faecalis after single- or multiple-visit root canal treatment Avaliação in vitro da efetividade do preparo químico-mecânico frente ao Enterococcus faecalis após tratamento endodôntico realizado em uma ou em múltiplas sessões

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    Eduardo Diogo Gurgel-Filho

    2007-12-01

    Full Text Available The purpose was to assess the elimination of Enterococcus faecalis in vitro in human mandibular premolars after chemomechanical preparation with or without the use of a calcium hydroxide dressing. After 60 days of contamination with E. faecalis, the root canals were prepared using the Crown-Down technique combined with 2% chlorhexidine gel irrigation. Then, the specimens were divided into two experimental groups, treated in a single visit or in multiple visits, and two control groups. The multiple-visit group received a dressing with calcium hydroxide for 14 days (CalenTM and the single-visit group did not receive any medication. In the two control groups, the canals were filled with BHI after chemomechanical preparation with 2% chlorhexidine gel or distilled water. Microbial samples were taken from the root canals for colony forming unit count for each phase of the treatment using sterile paper points inside the root canal lumen. Data were ranked and analyzed by the Kruskal-Wallis statistical test. The residual microbial colonies were then assessed. The results showed that chemomechanical preparation using 2% chlorhexidine gel with no intra-canal dressing reduced by 100% the E. faecalis contamination of the root canal lumen. The calcium-hydroxide group that received the 14-day intra-canal dressing allowed a small number of bacteria to grow between visits, but without statistical differences between groups.Objetivou-se avaliar in vitro a eliminação do Enterococcus faecalis em pré-molares inferiores humanos após o preparo químico-mecânico seguido ou não de curativo de hidróxido de cálcio. Após 60 dias de contaminação com E. faecalis os canais radiculares foram preparados utilizando-se a técnica coroa-ápice associada à irrigação com clorexidina em gel a 2%. Posteriormente os espécimes foram divididos em dois grupos experimentais, tratados em uma ou duas sessões, e dois grupos controles. O grupo tratado em duas sessões recebeu

  14. Crescimento radicular e aéreo de cultivares de arroz de terras altas em função da calagem Root and shoot growth of upland rice cultivars as affected by lime application

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    Carlos Alexandre Costa Crusciol

    2012-01-01

    Full Text Available O presente trabalho teve como objetivo avaliar o crescimento radicular e da parte aérea, além da eficiência de absorção de nutrientes pelas cultivares de arroz Caiapó e Maravilha, em função da calagem. O experimento foi desenvolvido em casa de vegetação, sendo os tratamentos constituídos pelas cultivares Caiapó e Maravilha combinadas com os índices de saturação por bases (V% de 10, 40 e 70. O delineamento experimental utilizado foi inteiramente casualizado, com quatro repetições. Foram avaliados o número de perfilhos, comprimento radicular, matéria seca de raízes, folhas e colmos, o teor foliar e a eficiência de absorção dos macronutrientes. De forma geral, observou-se que a cultivar moderna superou a tradicional em matéria seca de raízes, comprimento radicular, número de colmos, matéria seca de colmos e da parte aérea, além de acumular mais nutrientes a cultivar tradicional, mesmo nas condições de maior acidez. No entanto, os teores de nutrientes foram maiores na cultivar Caiapó. A cultivar Maravilha possui maior crescimento radicular e da parte aérea, nos diversos níveis de acidez, enquanto a Caiapó tem maior eficiência de absorção dos nutrientes do solo. A V% de 40 é a mais efetiva para ambas as cultivares.This work aimed to evaluate root growth, shoot development and nutrient absorption efficiency by rice cultivars Caiapó and Maravilha as affected by lime application. The experiment was carried out in a greenhouse, with treatments set up by Caiapó and Maravilha cultivars in combination with the base saturation (V% of 10%, 40% and 70%. The experiment was in a randomized complete block design, with four replications. The number of tillers, root length, root dry matter, leaves and stems, leaf content and macronutrients absorption efficiency were evaluated. Comparing cultivars, Maravilha cultivar presented higher root dry weight, root length, number of stems, dry matter of stems and shoot than Caiap

  15. Distribuição do sistema radicular de árvores de acáia-negra oriundas de mudas produzidas em diferentes recipientes Influence of seedling production method on the root system distribution of black wattle trees

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    Aline Fagote Paulino

    2003-10-01

    Full Text Available O objetivo deste trabalho foi estudar a distribuição do sistema radicular de árvores de acácia-negra (Acacia mearnsii com 3 anos de idade, provenientes de mudas formadas em diferentes recipientes e instaladas em um Argissolo no Rio Grande do Sul. Os recipientes utilizados foram: laminado, tubete redondo e bandeja de isopor. As avaliações da distribuição das raízes foram feitas pelo método da parede do perfil até 1 m de profundidade, na linha e na entrelinha de plantio. Foram feitas, também, análises físicas do solo: densidade, porosidade total e distribuição de poros. O recipiente usado na formação da muda influenciou o crescimento do sistema radicular da planta no campo. O laminado de madeira apresentou-se superior ao tubete quanto ao comprimento de raízes nas linhas de plantio. As raízes cresceram melhor nas linhas de plantio, onde as condições de densidade e porosidade do solo se encontravam mais adequadas.The purpose of this study was to evaluate the root system distribution of three-year-old black wattle (Acacia mearnsii trees. The plants were originated from seedlings grown in different containers and planted in an Argisol in the state of Rio Grande do Sul, Brazil. The containers used for seedling production were: wood laminated pots, round plastic tubettes and styrofoam trays. The evaluations were carried out using the trench profile method up to 1 m depth on tree rows and inter-rows. The following soil analyses were also carried out: bulk density, total porosity and pore distribution. The type of container used influenced the development of the root system of trees on the field. Wood laminated pots were better than the round tubettes for root length in the tree rows. The roots developed better on the tree rows where soil density and porosity conditions were more adequate.

  16. Pré-enraizamento de mudas de mandioquinha-salsa em diferentes bandejas e substratos com fungos micorrízicos arbusculares Pre-rooting of rhizomes of peruvian carrot in different trays and substrates with arbuscular micorrhizal fungi

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    Carla Andréia da Cunha Martins

    2007-02-01

    Full Text Available Realizou-se este trabalho com o objetivo de estudar a colonização radicular por fungos micorrízicos arbusculares (FMAs no período de pré-enraizamento da mandioquinha-salsa (Arracacia xanthorrhiza Bancroft. Os tratamentos constaram de dois substratos e dois tamanhos de bandeja de isopor. Utilizou-se um substrato comum constituído de 30% de composto orgânico, 30% de solo argiloso e 30% de areia e um substrato comercial Plantmax® Hortaliças; os tamanhos de bandejas de isopor foram: 128 células/bandeja (38 cm³ por célula e 200 células/bandeja (18 cm³ por célula. Efetuou-se uma inoculação mista de FMAs com solo inóculo composto pelas espécies Gigaspora margarita e Glomus clarum. Houve em ambos os substratos restrita resposta à inoculação dos FMAs, pela baixa colonização radicular, variando de 0,63 a 2,14% no substrato comercial e 7,93 a 15,09% no substrato comum. O substrato comum não apresentou características físicas desejáveis (aeração e drenagem para um bom desenvolvimento das raízes de mandioquinha-salsa durante a fase de pré-enraizamento. O substrato comercial apresentou maiores médias para a variável área e comprimento radicular em todas as coletas. A área radicular variou de 21,50 cm² com 30 DAP a 68,22 cm² com 60 DAP, enquanto o comprimento radicular variou de 2,64 m com 30 DAP a 12,64 m com 60 DAP. A bandeja de 200 células (18 cm³ célula/bandeja não foi adequada para a produção de mudas de mandioquinha-salsa.The objective of the work was to study the root colonization by arbuscular micorrhizal fungi (AMF during the development of Peruvian carrot rhizomes (Arracacia xanthorrhiza Bancroft. The treatments consisted of two substrates and two polystyrene trays sizes. A common substrate constituted of 30% of organic compost, 30% of loamy soil and 30% of sand, and a commercial substrate "Plantmax® Hortaliças"; and polystyrene trays sizes of 128 cells/tray (38 cm³ per cell and 200 cells/tray (18 cm

  17. Population and molecular genetics of root-knot nematodes

    NARCIS (Netherlands)

    Dautova, M.

    2001-01-01

    This thesis describes studies of root-knot nematodes <em>Meloidogyne> spp. - an economically important pest in agriculture - using population and molecular genetics. Variability in virulence to <em>Mi> bearing tomato genotypes is shown for

  18. Root abundance of maize in conventionally-tilled and zero-tilled soils of Argentina Abundância de raízes de milho (Zea mays L. em solos de Argentina sob preparo convencional e plantio direto

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    Miguel Angel Taboada

    2008-04-01

    Full Text Available Maize root growth is negatively affected by compacted layers in the surface (e.g. agricultural traffic and subsoil layers (e.g. claypans. Both kinds of soil mechanical impedances often coexist in maize fields, but the combined effects on root growth have seldom been studied. Soil physical properties and maize root abundance were determined in three different soils of the Rolling Pampa of Argentina, in conventionally-tilled (CT and zero-tilled (ZT fields cultivated with maize. In the soil with a light Bt horizon (loamy Typic Argiudoll, Chivilcoy site, induced plough pans were detected in CT plots at a depth of 0-0.12 m through significant increases in bulk density (1.15 to 1.27 Mg m-3 and cone (tip angle of 60 º penetrometer resistance (7.18 to 9.37 MPa in summer from ZT to CT, respectively. This caused a reduction in maize root abundance of 40-80 % in CT compared to ZT plots below the induced pans. Two of the studied soils had hard-structured Bt horizons (clay pans, but in only one of them (silty clay loam Abruptic Argiudoll, Villa Lía site the expected penetrometer resistance increases (up to 9 MPa were observed with depth. In the other clay pan soil (silty clay loam Vertic Argiudoll, Pérez Millán site, penetrometer resistance did not increase with depth but reached 14.5 MPa at 0.075 and 0.2 m depth in CT and ZT plots, respectively. However, maize root abundance was stratified in the first 0.2 m at the Villa Lía and Pérez Millán sites. There, the hard Bt horizons did not represent an absolute but a relative mechanical impedance to maize roots, by the observed root clumping through desiccation cracks.O crescimento das raízes do milho é alterado negativamente quando o solo apresenta camadas compactadas superficiais (trânsito de maquinário ou subsuperficiais (camada argilosa. Os dois tipos de impedimentos mecânicos muitas vezes coexistem nos campos sob cultura de milho, porém seus efeitos combinados no crescimento das raízes do milho

  19. Comparative Vector Bionomics and Morphometrics of Two Genetically Distinct Field Populations of Anopheles darlingi Root from Belize, Central America and Zungarococha, Peru, South America

    Science.gov (United States)

    2014-01-31

    and malaria transmission in the Upper Orinoco River , Southern Venezuela. Memorias do Instituto Oswaldo Cruz 102:303-11 112. Maheu-Giroux M, Casapia M...transmission in remote areas of human occupation. Sites along the Mazan River , utilized by many laborers to fish, extract wood, and harvest palm leaves...include lagoons, lakes, swamps, and slow flowing rivers or streams (83; 171). Often larvae are associated with floating debris or detritus patches

  20. Root patterning

    NARCIS (Netherlands)

    Scheres, Ben; Laskowski, Marta

    2016-01-01

    The mechanisms that pattern lateral root primordial are essential for the elaboration of root system architecture, a trait of key importance for future crop breeding. But which are most important: periodic or local cues? In this issue of Journal of Experimental Botany (pages 1411-1420), Kircher

  1. Poda de raízes e adubação para crescimento do cafeeiro cultivado em colunas de solo Root pruning and fertilization for growth of coffee plants cultivated in soil columns

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    Víctor Hugo Alvarez V.

    2006-02-01

    Full Text Available Em casa de vegetação, foram estudados os efeitos de níveis de fertilidade, poda de raízes e adubação localizada sobre o crescimento da parte aérea e do sistema radicular de plantas de cafeeiro cultivar Catuaí Vermelho. Amostras subsuperficiais (30 a 70 cm de um LVA de Viçosa-MG foram acondicionadas em colunas de PVC compostas por três anéis de 15 cm de altura e 20 cm de diâmetro. Quatro tratamentos com três níveis de fertilidade [baixo (FB; médio (FM; alto (FA e médio sem corte de raízes, em apenas dois anéis (FM2], foram instalados antes do plantio, utilizando três níveis de calagem e três doses de esterco de galinha. As doses de P e K aplicadas no plantio foram inversamente proporcionais aos níveis de fertilidade propostos. Oito meses após o plantio, o anel inferior foi retirado, podando-se o sistema radicular. As amostras de solo do anel inferior foram substituídas por novas amostras nas quais foram aplicadas quatro adubações localizadas [baixa (AB; média (AM; alta (AA e média mais esterco de galinha (AM2]. Dezessete meses após o plantio, foram feitas avaliações da parte aérea e do sistema radicular. A altura das plantas e o número de ramos decresceram linearmente com o nível de fertilidade, em razão das menores doses de P e K adicionadas na adubação de plantio. Esta tendência também foi observada na produção de matéria seca da parte aérea; entretanto, o crescimento do sistema radicular não foi afetado. A poda de raízes não influiu no crescimento e na produção de matéria seca da parte aérea e das raízes, exceto quando se adicionou esterco de galinha na adubação localizada no nível de fertilidade média, promovendo, neste caso, efeito negativo. As adubações localizadas no nível de fertilidade baixo não afetaram o crescimento da parte aérea nem do sistema radicular; no nível médio, verificou-se efeito quadrático positivo e, no nível alto, observou-se aumento linear no crescimento

  2. Enraizamento de miniestacas de espinheira-santa (Maytenus ilicifolia Mart. ex Reissek em diferentes substratos Rooting of espinheira-santa (Maytenus ilicifolia Mart. ex Reissek minicuttings on different substrates

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    Daniela Macedo de Lima

    2009-04-01

    Full Text Available Pertencente à família Celastraceae, a espinheira-santa (Maytenus ilicifolia Mart. ex Reissek é uma espécie medicinal nativa da região sul do Brasil, utilizadas no tratamento de gastrite e úlceras gástricas. Estudos sobre a produção de mudas por meio de estaquia demonstraram que esta espécie é considerada de difícil enraizamento. Objetivou-se, no presente trabalho, avaliar os efeitos de diferentes substratos no enraizamento de miniestacas de espinheira-santa. Miniestacas apicais foram coletadas a partir das brotações das minicepas de 10 meses de idade, mantidas em casa-de-vegetação e confeccionadas com 3 a 4cm de comprimento, mantendo-se duas folhas com a superfície reduzida à metade. O plantio foi realizado em caixas contendo areia e tubetes contendo casca de arroz carbonizada, Plantmax HT® ou vermiculita de granulometria fina como substrato. As miniestacas foram mantidas em casa-de-vegetação com nebulização intermitente e 90 dias após o plantio, foram avaliadas as características: porcentagem de estacas enraizadas, número e comprimento médio de raízes, porcentagem de estacas com calos, sobrevivência e mortalidade. O delineamento experimental foi inteiramente casualizado, com 4 tratamentos e 4 repetições de 22 estacas por parcela. A análise estatística revelou que os substratos areia (92,04%, casca de arroz carbonizada (88,66% e Plantmax HT® (94,31% apresentaram índices de enraizamento significativamente superiores à vermiculita (78,41%, além de menores taxas de mortalidade, sendo que a propagação vegetativa de espinheira-santa por meio de miniestaquia é viável.Espinheira-santa (Maytenus ilicifolia Mart. ex Reissek, which belongs to the Celastraceae family, is a medicinal species native to the south region of Brazil, used in gastritis and gastric ulcer treatment. Studies about plant production by cutting have shown that this species is considered difficult to root. The purpose of this work was to evaluate

  3. Evolução da anatomia radicular do milho 'Saracura' em ciclos de seleção sucessivos Evolution of the root anatomy of 'Saracura' maize in successive selection cycles

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    Fabricio José Pereira

    2008-12-01

    Full Text Available Este trabalho teve como objetivo verificar as modificações nas características anatômicas radiculares relacionadas à hipoxia, durante 18 ciclos de seleção da variedade de milho (Zea mays BRS 4154 Saracura, adaptada a áreas sujeitas a alagamento. A variedade BR 107 e o híbrido simples BRS 1010, suscetíveis ao alagamento, foram utilizados como controle. As diferentes cultivares foram submetidas a alagamentos intermitentes, a cada dois dias, durante dois meses. As amostras radiculares foram preparadas e analisadas em microscopiaóptica. Em relação ao grupo controle e aos ciclos anteriores de seleção, a BRS 4154 teve aumento na formação de aerênquima, diminuição do córtex, diminuição do diâmetro dos vasos, diminuição da camada subepidérmica, aumento na espessura do floema e epiderme. Os sucessivos ciclos de seleção melhoraram as características do milho 'Saracura' e sua tolerância a ambientes alagados.This work aimed to verify the modifications in the root anatomical characteristics related to hypoxia, along 18 selection cycles of the maize (Zea mays cultivar BRS 4154 Saracura, adapted to areas subject to flooding. Cultivars not tolerant to fooding, BR 107 and the simple hybrid BRS 1010, were used as controls. The different cultivars were subjected to intermittent soil waterlogging every two days for two months. The root samples were prepared and analyzed through light microscopy. Compared to the controls group and to early selection cycles, BRS 4154 had increased development of aerenchyma, decreased cortex thickness, decreased metaxylem diameter, decreased subepidermal layer, and increased epidermis and phloem thickness. The successive selection cycles improved 'Saracura' maize characteristics and its tolerance to flooding environments.

  4. Densidade radicular de progênies de pupunheira em função de adubação NPK Root density of pejibaye progenies as a function of NPK fertilization

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    Marilene L. A. Bovi

    1999-11-01

    Full Text Available O uso eficiente de fertilizantes requer o conhecimento de seus efeitos tanto na biomassa aérea quanto na radicular, permitindo que se identifique uma adubação adequada do ponto de vista de uma melhor partição entre ambas. A massa de raízes secas por volume de solo vem sendo utilizada, principalmente em espécies perenes, como subsídio para estimativas de biomassa radicular. Com esse objetivo, foram estudados os efeitos da adubação NPK na densidade radicular de três progênies de pupunheira (Bactris gasipaes Kunth, cultivadas em um Aluvial álico (corrigido por meio de calagem em Ubatuba, SP (clima "Cfa", durante o ano de 1993. O delineamento foi um fatorial fracionado, composto por quatro doses crescentes de nitrogênio (0 a 400 kg/ha/ano de N, fósforo (0 a 200 kg/ha/ano de P2O5 e potássio (0 a 200 kg/ha/ano de K2O, em experimento integrado (progênies e adubação. As amostras foram coletadas por ocasião da primeira colheita de palmito (outubro de 1993, quando as plantas (cultivadas no espaçamento de 2 x 1 m tinham cerca de dois anos de campo. Utilizou-se o método do trado, coletando-se, por parcela experimental, duas amostras na linha e duas na entrelinha. Houve diferenças entre genótipos (GE, com as progênies 2 e 3 apresentando, em média, maior densidade radicular que a progênie 1 (5,15 e 6,20 contra 2,61 g/dm³, respectivamente. A interação GE x PO (posição da amostra foi significativa, sendo que para as progênies 1 e 2 a maior densidade radicular foi obtida na linha (3,30 e 6,60 g/dm³ na linha, contra 1,92 e 4,70 g/dm³ na entrelinha, respectivamente, enquanto para a 3 a ordem foi inversa (5,57 g/dm³ na linha e 6,84 g/dm³ na entrelinha. Doses crescentes de potássio (K apresentaram efeitos lineares positivos e significativos (R² =0,93 a 0,97 na densidade radicular das três progênies, com acréscimos entre dose mínima e máxima variando de 18 a 28%. Não houve efeito significativo isolado de P, enquanto doses

  5. Sazonalidade na produção de raízes e teor de β-ecdisona em acessos de fáfia Seasonal variation in root production and β-ecdisone content in pfaffia accessions

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    Cirino Corrêa Júnior

    2008-09-01

    Full Text Available As raízes de espécies de Pfaffia são utilizadas há muito na medicina popular no Brasil, especialmente como tônicas, afrodisíacas e antidiabéticas. Nas margens e ilhas do Rio Paraná, vegeta naturalmente a espécie Pfaffia glomerata (Spreng. Pedersen, que corre risco de extinção pela coleta intensiva de suas raízes. Para preservá-la, é preciso desenvolver sistemas que permitam seu uso sustentável. Nesse contexto, foi avaliada a sazonalidade da produção de biomassa e o teor de β-ecdisona em raízes de diferentes acessos de P. glomerata. A coleta dos acessos foi realizada ao longo dos rios Paraná (A1 e A3, Ivaí (A2 e Paranapanema (A4. Mudas produzidas a partir desses acessos foram plantadas experimentalmente em área de ocorrência natural da espécie. O delineamento utilizado foi blocos casualizados, com parcelas subdivididas (acessos nas parcelas; épocas de colheita nas subparcelas, quatro repetições e subparcelas com seis plantas na área útil. Foram realizadas quatro colheitas: oito, dez, doze e quatorze meses após o plantio. As maiores produtividades em massa seca (MS de raízes foram alcançadas na terceira colheita, com 38,41 g planta-1. Já o acesso A4 foi significativamente mais produtivo que os demais, tanto para massa fresca (94,67 g planta-1, quanto para MS de raízes (26,39 g planta-1. A relação MF/MS das raízes, com média de 26,9% (3,7:1, foi pouco influenciada pela origem dos acessos, embora tenha se alterado significativamente ao longo das colheitas (de 4,1:1, na primeira colheita, a 3,3:1, na terceira colheita, que correspondeu ao valor máximo. O teor de β-ecdisona foi estatisticamente similar nas três primeiras colheitas, variando de 0,26 a 0,38%, com redução drástica na última colheita (0,16%. Os acessos A2 e A3 foram aqueles com teor de β-ecdisona mais altos, respectivamente 0,36 e 0,30%. Considerando os resultados obtidos, sugere-se que a terceira colheita, que corresponde a doze meses após o

  6. Transporte de enxofre para as raízes de soja em três solos de Minas Gerais Sulphur transport toward soybean roots in three soils from Minas Gerais State, Brazil

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    Davi José Silva

    2002-08-01

    Full Text Available Com o objetivo de avaliar a contribuição do fluxo em massa e da difusão no transporte de S para a superfície das raízes de soja, conduziu-se um ensaio em casa de vegetação, com amostras de três solos dos municípios de Viçosa, Paracatu e Lassance. Essas amostras apresentavam, respectivamente, 5,0, 1,2 e 1,4 mg dm-3 de S disponível, obtidos pelo extrator Ca(H2PO42, 500 mg L-1 de P em HOAc 2 mol L-1. O experimento correspondeu a um fatorial 3 x 5, sendo três solos e cinco doses de S (0, 20, 40, 80 e 160 mg dm-3, dispostos em blocos casualizados, com quatro repetições. A umidade, controlada pelo uso de um tensiômetro por vaso, foi mantida próximo a -10 kPa durante todo o ensaio. O fluxo em massa foi o principal mecanismo de transporte de S para a superfície das raízes da soja. Quando a concentração de S na solução do solo foi alta, esse mecanismo supriu quantidades de S superiores às absorvidas pela planta. A contribuição da difusão para o suprimento de S ocorreu apenas em baixa concentração desse nutriente na solução do solo.A greenhouse experiment was carried out to evaluate the mass flow and diffusion contributions on sulphur transport toward soybean roots in soil surface samples (0-20 cm of three soils collected in Viçosa, Paracatu and Lassance, Minas Gerais State, Brazil. Originally, the soil samples presented 5.0, 1.2 and 1.4 mg dm-3 of available S, respectively, obtained by extraction with a Ca(H2PO42 solution containing 500 mg L-1 of P in HOAc 2 mol L-1 solution. A 3x5 factorial layout was used with three soils and five S rates (0, 20, 40, 80 and 160 mg dm-3, disposed in a randomized complete block design, with four replications. Tensiometers controlled the soil water potential in each pot and the potential was maintained around -10 kPa. The mass flow was the main mechanism of S transport to soybean roots. At high concentration in soil solution, the quantities supplied by mass flow were higher than those

  7. Lime and gypsum to improve root depth of orange crop in an Ultisol of the Coastal Tablelands Calcário e gesso no aprofundamento radicular da laranjeira em um Argissolo dos Tabuleiros Costeiros

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    Lafayette F. Sobral

    2009-12-01

    Full Text Available Coastal Tableland is a landscape unit in the North East of Brazil in which the main soils are Ultisols. In these soils, a compacted layer denominated "cohesive horizon" occurs and root growth is limited by it. An experiment with five treatments and six replications was set up in order to study how liming and gypsum could improve root depth of orange (Citrus sinensis L. Osbeck crop in an Ultisol in which a compacted layer was found at 0.3 m. Treatments were: A - No liming and no gypsum; B - Liming to achieve 60% base saturation; C - B + 1 t of gypsum ha-1 ; D - B + 2 t of gypsum ha-1 and E - B + 3 t of gypsum ha-1. Gypsum increased calcium and sulfate in the cohesive horizon. Surface application of lime and gypsum did not cause changes in soil density and total porosity in the cohesive horizon. An improvement of root length was observed at the cohesive horizon.Os tabuleiros costeiros são uma unidade de paisagem em que um dos principais solos são os Argissolos, nos quais, uma camada compactada, denominada "horizonte coeso" ocorre e o crescimento radicular é por ela limitado. Um experimento com cinco tratamentos e seis repetições foi implantado para se estudar os efeitos da calagem e do gesso no aprofundamento radicular da laranjeira (Citrus sinensis L. Osbeck em um Argissolo onde o horizonte coeso está a 0,3 m de profundidade. Os tratamentos foram: A - Sem gesso e sem calagem; B - Calagem para atingir 60% de saturação por bases; C - B + 1 t ha-1 de gesso ; D - B + 2 t ha-1 de gesso; e E - B + 3 t ha-1 de gesso. A calagem e o gesso aumentaram significativamente os teores de sulfato e de calcio no solo até a profundidade de 0,40 m. A aplicação a lanço de calcário e gesso não causaram modificações na densidade do solo e na porosidade total da camada compactada "horizonte coeso". Foi observado um aumento do comprimento das raízes da laranjeira na camada compactada.

  8. Root resorption

    DEFF Research Database (Denmark)

    Kjaer, Inger

    2014-01-01

    Introduction: This paper summarizes the different conditions, which have a well-known influence on the resorption of tooth roots, exemplified by trauma and orthodontic treatment. The concept of the paper is to summarize and explain symptoms and signs of importance for avoiding resorption during...... orthodontic treatment. The Hypothesis: The hypothesis in this paper is that three different tissue layers covering the root in the so-called periroot sheet can explain signs and symptoms of importance for avoiding root resorption during orthodontic treatment. These different tissue layers are; outermost......-an ectodermal tissue layer (Malassez′s epithelium), a middle layer-composed by the collagen-mesodermal tissue layer, and an innermost root-close innervation layer. Abnormalities in one of these tissue layers are thought to cause inflammatory processes in the periodontal membrane comparable to inflammatory...

  9. Licorice Root

    Science.gov (United States)

    ... T U V W X Y Z Licorice Root Share: On This Page Background How Much Do ... This fact sheet provides basic information about licorice root—common names, usefulness and safety, and resources for ...

  10. Root growth and lignification of glyphosate susceptible and resistant soybean at low temperaturesCrescimento e lignificação de raízes de soja convencional e resistente ao glifosato, em baixa temperatura

    Directory of Open Access Journals (Sweden)

    Patricia da Costa Zonetti

    2013-05-01

    Full Text Available Low temperature stress affects plant growth, including primary and secondary metabolism. Glyphosateresistant soybean contains a modified DNA, which encodes a different type of secondary metabolism enzyme related to lignin synthesis compared to conventional glyphosate-susceptible cultivars. Thus, this soybean cultivar might respond differently to low temperatures, compared to glyphosate-susceptible cultivars. This work aimed to investigate how decreasing temperatures influence the growth and lignin content of the glyphosate-resistant soybean compared to its susceptible parental cultivars. Three-day-old seedlings were cultivated in nutrient solution at 10, 15, 20, and 25°C (±2°C, using a 12-h photoperiod. After 96 h, taproot growth, fresh and dry biomass, and lignin levels were determined. The results indicate that lower temperatures decreased seedling and root growth in both types of cultivars; however, glyphosate-resistant soybean exhibited greater root length, biomass, and lignin content compared to the glyphosate-susceptible parental cultivar. O estresse causado pela baixa temperatura, dentre outras implicações, afeta o crescimento do vegetal assim como o seu metabolismo secundário. Pelo fato da soja RR apresentar variante enzimática de uma das principais vias do metabolismo secundário, ligada à síntese de lignina, pode apresentar comportamento diferenciado, sob baixa temperatura, se comparada com sua linhagem parental. O objetivo deste trabalho foi investigar possíveis diferenças no crescimento e nos conteúdos de lignina nas raízes de soja cultivar transgênica resistente ao glifosato e cultivar parental em resposta a redução de temperatura. Após três dias de germinação das sementes, as plântulas foram mantidas em solução nutritiva, a 10, 15, 20 e 25°C (±2°C, com fotoperíodo de 12 horas. Após 96 horas, foi avaliado o comprimento relativo da raiz primária, biomassa fresca e seca das raízes e os teores de lignina

  11. Composição de ácidos graxos em raízes de sorgo sob estresse de alumínio Fatty acid composition of sorghum roots under aluminum stress

    Directory of Open Access Journals (Sweden)

    Paulo Henrique Pereira Peixoto

    2009-01-01

    Full Text Available Este trabalho teve por objetivo determinar a influência do Al sobre a composição de ácidos graxos em raízes de duas cultivares de sorgo [Sorghum bicolor (L. Moench] com tolerância diferencial ao Al. Após exposição das plantas ao Al, raízes foram coletadas, os lipídios extraídos e determinada sua composição em ácidos graxos. O palmítico e, especialmente, o linoléico foram os principais ácidos graxos identificados nos extratos lipídicos de raízes das duas cultivares, independentemente da presença do Al. Nas plantas tratadas com Al, os teores dos ácidos esteárico e oléico aumentaram apenas na cultivar tolerante, mas os teores do ácido linolênico reduziram nas duas cultivares. Essas alterações na composição de ácidos graxos, o índice de ligações duplas para ácidos graxos contendo 18 carbonos (ILD C18 e as relações ácidos graxos saturados/ácidos graxos insaturados (AGI/AGS e ácido linoléico/ácido palmítico (18:2/16:0 sugerem que ocorre redução na fluidez dos lipídios da cultivar tolerante, porém aumento na sensível. A redução na fluidez dos lipídios na cultivar tolerante, comparativamente à da sensível sugere ter a cultivar tolerante maior capacidade de reter íons e metabólitos essenciais e, ou limitar a entrada do Al em suas raízes.The objective of this work was to determine Al effect on fatty acid composition of roots from two sorghum (Sorghum bicolor (L. Moench cultivars with differential tolerance to aluminum. After exposure to Al, roots were collected, lipids extracted and the fatty acid composition determined. Palmitic and, especially, linoleic acid were the major fatty acids identified in the root lipid extracts of both cultivars, regardless of the presence of aluminum. Stearic and oleic acids contents increase only in the tolerant cultivar, while linolenic acid decreased in both cultivars after Al treatment. These changes in fatty acid composition, as well as in the C18-fatty acid double

  12. Root nodule organogenesis : molecular characterization of the zonation central tissue

    NARCIS (Netherlands)

    Yang, W.C.

    1994-01-01

    Legume plants form root nodules by interacting with the soil bacterium, <em>Rhizobium.> In these nodules bacteria are able to convert atmospheric nitrogen into ammonia which is used by the host plants as nitrogen source. Therefore symbiotic nitrogen fixation in root nodules

  13. Distribuição do sistema radicular da bananeira 'Prata-Anã' em duas freqüências de fertirrigação com uréia Distribution of the 'Prata Anã' root system in two urea fertirrigation frequencies

    Directory of Open Access Journals (Sweden)

    Ana Lúcia Borges

    2008-03-01

    Full Text Available O conhecimento da distribuição das raízes da bananeira subsidia informações para orientar a melhor localização de água e fertilizantes, resultando em incremento da produtividade do pomar. Em Latossolo Amarelo distrófico argissólico, em sistema de fileiras duplas (4 x 2 x 2 m, avaliou-se a distribuição do sistema radicular da bananeira 'Prata-Anã', antes da colheita do 2º ciclo, em duas freqüências de fertirrigação com uréia (400 kg de N/ha/ano, a cada 3 dias (F1 e a cada 15 dias (F2. As raízes foram coletadas em diferentes profundidades e distâncias, utilizando tubo metálico, sendo separadas do solo e quantificadas pelo programa GS Root. A maior freqüência de aplicação de N e de água (3 dias favoreceu a densidade de raízes, em comparação com a menor freqüência (15 dias. A maior concentração de raízes ocorreu nas camadas superficiais, até 0,30 m, e entre a planta e o microaspersor. Predominaram raízes de diâmetro entre 0,2 e >1,5 mm, tanto nas camadas superficiais (0 a 0,20 m de profundidade quanto entre a planta e o microaspersor.The knowledge of the distribution of roots in bananas generates information that guide the location of water and fertilizers, resulting an increase in crop yield in banana plantations. The 'Prata Anã' banana root distribution system, in loam clay sandy Yellow Latosol, before harvest of the second cycle, was evaluated in two urea fertirrigation frequencies (400 kg of N/ha/year every 3 days (F1 and 15 days (F2. Root samples were collected in different distances and depths with a metallic tube, separated from the soil and quantified using the GSRoot software. Higher urea fertirrigation frequency (3 days favored root density, in relation to the 15 days frequency. The amount of roots in the superficial layers (up to 0.30 m was greater, decreasing with the depth, as well as for the plant in the direction of the micro sprinkler, and less in the opposite direction. Roots of diameter

  14. Peroxidase activity in roots of arracacha affected by pH and temperature = Atividade da peroxidase em raízes de batata-baroa afetada pelo pH e temperatura

    Directory of Open Access Journals (Sweden)

    Luciana Nunes Menolli

    2011-07-01

    Full Text Available In this paper, roots of arracacha (Arracacia xanthorrhyza Bancroft were stored at 5ºC to induce chilling injury symptoms and stress-related peroxidase activity. Later, peroxidase kinetic activity was determined in different pH and temperature conditions. For this, soluble crude extract was sequentially saturated with ammonium sulfate, obtaining a semi-purified enzyme solution used for the analysis. Activity of peroxidase induced by the chilling at 5oC was determined from pH 2.5 to 9.0 and at temperature ranging from 10 to80oC. The peroxidase had higher activity when the reaction occurred between pH 5.5 and 6.0 and at temperature of 30oC. Complete inactivation of the activity was observed in pH 2.5 after 60 minutes of pre-incubation or at 60oC for 10 minutes or alternatively at 70oCafter 5 minutes of pre-incubation. The enzyme is more susceptible to inactivation in acid than alkaline pHs or alternatively using heat treatment.Neste trabalho, raízes de batata-baroa (Arracacia xanthorrhiza Bancroft foram armazenadas a 5oC para induzir injúria por frio e expressar atividade da peroxidase de estresse. Posteriormente, a cinética de atividade foi determinada em diferentes condições depHs e temperatura. Para isto, extrato solúvel da raiz foi sequencialmente saturado com sulfato de amônio, obtendo-se uma preparação semi-purificada para a análise enzimática. Atividade peroxidativa induzida pela temperatura de armazenamento de 5oC foideterminada em pHs de 2,5 a 9,0 e a temperaturas de 10 a 80oC. A atividade da peroxidase foi maior quando a reação foi realizada nos pHs de 5,5 e 6,0 e temperatura de 30oC. A inativação completa da enzima ocorreu em pH de 2,5 após 60 min. de pré-incubação ou a60oC por 10 min., e alternativamente a 70oC após 5 min. de pré-incubação. A enzima foi mais susceptível à inativação em pH ácido do que alcalino, podendo também ser inativada pelo tratamento de calor.

  15. Effects of dorsal root ganglion destruction by adriamycin in patients with postherpetic neuralgia Efeitos da destruição da raiz dorsal ganglionar pela adriamicina em pacientes com neuralgia pós-herpética

    Directory of Open Access Journals (Sweden)

    He Chun-jing

    2012-06-01

    Full Text Available PURPOSE: To investigate the effects of dorsal root ganglion destruction in patients with postherpetic neuralgia (PHN. METHODS: Seventy-two patients with PHN selected were randomly divided into two groups (n=36. Group A was the control group (treated by injection and group B was the group of dorsal root ganglion destruction by adriamycin. Visual analog scale scores (VAS, SAS, SF-MPQ scores. Clinical effects and therapy safety were evaluated before therapy, one week, three and six months after therapy. Forty-four patients were available for intention-to-treat analysis. RESULTS: The average pain scores on the Likert scale were significantly reduced at each point in group B. Patients in group B reported clinical effectiveness at six months as excellent response, good response, improved but unsatisfactory or unchanged 16, 12 and 8.VAS scores at each time point after the operation were lower than that before operation and in group A, there was significant difference. Patients showed significant improvement in sleep scores in group B. There was significant difference at T2 in group A than T1. There was no significant difference in group A at T3, T4 after the operation than that before operation. Between group comparison: there was significant difference between group A and group B at each time point after the operation. CONCLUSIONS: Dorsal root ganglion destruction by adriamycin under guidance of C-arm perspective, the puncture operation was accurate without any adverse reaction or serious complications, which could effectively relieve pain of patients with postherpetic neuralgia, but the long-term effects needed further study.OBJETIVO: Investigar os efeitos da destruição da raiz dorsal ganglionar em pacientes com neuralgia pós-herpética. MÉTODOS: Setenta e dois pacientes selecionados com neuralgia pós-herpética foram randomicamente distribuídos em dois grupos (n=36. Grupo A foi o grupo controle (tratado por injeção e o grupo B foi o grupo

  16. Crescimento radicular de soja em razão da sucessão de cultivos e da compactação do solo Soybean root growth as affected by previous crop and soil compaction

    Directory of Open Access Journals (Sweden)

    Rosemeire Helena da Silva

    2002-06-01

    Full Text Available O objetivo do experimento foi avaliar o crescimento radicular e produção de matéria seca da parte aérea da soja (Glycine max (L. Merrill cultivada após diversas espécies vegetais, em solo com diferentes níveis de compactação. O trabalho foi realizado em vasos contendo amostras de um Latossolo Vermelho, textura franco arenosa, com camada de 3,5 cm (profundidade de 15 a 18,5 cm compactada até as densidades 1,12, 1,36 e 1,60 Mg m-3, onde cultivaram-se anteriormente aveia-preta, guandu, milheto, mucuna-preta, soja, sorgo granífero e tremoço-azul, e um tratamento sem planta (pousio. Essas espécies se desenvolveram por 37 a 39 dias, foram cortadas ao nível do solo, picadas em partes de aproximadamente 3 cm e deixadas sobre a superfície do vaso por 40 dias. Após esse período, cultivou-se a soja até 28 dias após a emergência, quando, então, as plantas foram colhidas. Foram avaliados produção de matéria seca da parte aérea e de raízes, e comprimento e diâmetro radicular da soja. O cultivo anterior com aveia-preta, guandu e milheto favoreceu o crescimento radicular da soja abaixo de camadas compactadas do solo. Independentemente do nível de compactação, o cultivo anterior com qualquer das espécies estudadas beneficiou a produção de matéria seca da parte aérea da soja.This study aimed at evaluating root growth and shoot dry matter production of soybean (Glycine max (L. Merrill cropped after different vegetal species, in a soil with different compaction levels. The experiment was conducted in pots containing a Dark-Red Latosol (Acrortox, loamy sand, and the pots had a layer 3.5 cm (15 to 18.5 cm thick and 15 cm deep compacted to 1.12, 1.36 and 1.60 Mg m-3. Before soybean, the pots were cropped with black oat, pigeon pea, pearl millet, black mucuna, soybean, grain sorghum and lupin, plus a treatment without plants. These species were grown for 37 to 39 days, when they were cut at soil level, prick in particles of

  17. Evaluation of apical microleakage of teeth sealed with four different root canal sealers Avaliação da microinfiltração apical em dentes obturados com quatro diferentes cimentos endodônticos

    Directory of Open Access Journals (Sweden)

    Fábio Dultra

    2006-10-01

    Full Text Available OBJECTIVES: to compare the apical sealing ability of four root canal sealers. MATERIAL AND METHODS: forty extracted human maxillary canines were instrumented 1 mm short of the anatomical apex and randomly assigned to four groups (n=10, according to the root canal sealer used for obturation: Endofill, AH Plus, EndoREZ and Epiphany. Root canals were obturated with gutta-percha points, except for the Epiphany group, in which resin points (Resilon were used. The teeth were immersed in India ink for seven days and clarified using methyl salicylate. The extent of apical dye penetration was measured with a measuroscope in all aspects of the canal. RESULTS: AH Plus (0.02 mm ± 0.07, Epiphany (0.00 mm ± 0.00 and EndoREZ (0.32 mm ± 0.62 did not differ statistically to each other (p>0.01. EndoFill presented the highest dye penetration mean (0.83 mm ± 0.73 and was statistically different from the other sealers (pOBJETIVOS: comparar a capacidade de selamento apical de quatro cimentos endodônticos. MÉTODOS: quarenta caninos superiores humanos extraídos foram instrumentados 1 mm aquém do ápice anatômico e distribuídos aleatoriamente em quatro grupos (n=10, de acordo com o cimento endodôntico utilizado para a obturação: Endofill, AH Plus, EndoREZ e Epiphany. Os canais radiculares foram obturados pela técnica da condensação lateral com os cimentos e com cones de guta-percha, exceto o grupo do Epiphany, no qual os cones de resina (Resilon foram utilizados. Os dentes foram imersos em nanquim por sete dias e submetidos ao processo de diafanização e, então, clarificados empregando-se o salicilato de metila. A extensão de penetração via apical do corante foi medida por meio de um microscópio de mensuração em todas as faces do terço apical. RESULTADOS: AH Plus (0,02 mm ± 0,07, Epiphany (0,00 mm ± 0,00 e EndoREZ (0,32 mm ± 0,62 não diferiram estatisticamente entre si (p>0,01. EndoFill apresentou a maior média de penetração do corante (0

  18. Root distribution of irrigated grapevine rootstocks in a coarse texture soil of the São Francisco Valley, Brazil Distribuição radicular de porta-enxertos de videiras irrigadas em um solo de textura arenosa do Vale do São Francisco

    Directory of Open Access Journals (Sweden)

    LUÍS HENRIQUE BASSOI

    2002-04-01

    Full Text Available An experiment was carried out to determine the root distribution of four grapevine rootstocks (Salt Creek, Dogridge, Courdec 1613, IAC 572 in a coarse texture soil of a commercial growing area in Petrolina County, São Francisco Valley, Brazil. Rootstocks were grafted to a seedless table grape cv. Festival, and irrigated by microsprinkler. Roots were quantified by the trench wall method aided by digital image analysis. Results indicated that roots reached 1 m depth, but few differences among rootstocks were found. All of them presented at least 90 % of the roots distributed until 0.6 m depth, with a greater root presence in the first 0.4 m. The upper 0.6 m can be taken into account as the effective rooting depth for soil and water management.Um experimento foi conduzido para se determinar a distribuição radicular de quatro porta-enxertos (Salt Creek, Dogridge, Courdec 1613, IAC 572 em um solo de textura arenosa, em um plantio comercial em Petrolina - PE, no Vale do São Francisco. Os porta-enxertos foram enxertados com a cv. de uva de mesa sem sementes Festival, e irrigados por microaspersão. As raízes foram quantificadas pelo método da parede da trincheira auxiliado pela análise de imagem digital. Os resultados indicaram que as raízes atingiram 1 m de profundidade, mas poucas diferenças entre os porta-enxertos foram observadas. Cerca de 90% do sistema radicular de todos os porta-enxertos estavam distribuídos até 0,6 m de profundidade, mas houve uma grande presença de raízes até 0,4 m. A camada superficial de solo de 0,6 m pode ser considerada como a profundidade efetiva do sistema radicular para fins de manejo de solo e água.

  19. Root (Botany)

    Science.gov (United States)

    Robert R. Ziemer

    1981-01-01

    Plant roots can contribute significantly to the stability of steep slopes. They can anchor through the soil mass into fractures in bedrock, can cross zones of weakness to more stable soil, and can provide interlocking long fibrous binders within a weak soil mass. In deep soil, anchoring to bedrock becomes negligible, and lateral reinforcement predominates

  20. Automated Root Tracking with "Root System Analyzer"

    Science.gov (United States)

    Schnepf, Andrea; Jin, Meina; Ockert, Charlotte; Bol, Roland; Leitner, Daniel

    2015-04-01

    Crucial factors for plant development are water and nutrient availability in soils. Thus, root architecture is a main aspect of plant productivity and needs to be accurately considered when describing root processes. Images of root architecture contain a huge amount of information, and image analysis helps to recover parameters describing certain root architectural and morphological traits. The majority of imaging systems for root systems are designed for two-dimensional images, such as RootReader2, GiA Roots, SmartRoot, EZ-Rhizo, and Growscreen, but most of them are semi-automated and involve mouse-clicks in each root by the user. "Root System Analyzer" is a new, fully automated approach for recovering root architectural parameters from two-dimensional images of root systems. Individual roots can still be corrected manually in a user interface if required. The algorithm starts with a sequence of segmented two-dimensional images showing the dynamic development of a root system. For each image, morphological operators are used for skeletonization. Based on this, a graph representation of the root system is created. A dynamic root architecture model helps to determine which edges of the graph belong to an individual root. The algorithm elongates each root at the root tip and simulates growth confined within the already existing graph representation. The increment of root elongation is calculated assuming constant growth. For each root, the algorithm finds all possible paths and elongates the root in the direction of the optimal path. In this way, each edge of the graph is assigned to one or more coherent roots. Image sequences of root systems are handled in such a way that the previous image is used as a starting point for the current image. The algorithm is implemented in a set of Matlab m-files. Output of Root System Analyzer is a data structure that includes for each root an identification number, the branching order, the time of emergence, the parent

  1. Gene-for-gene relationships between strawberry and the causal agent of red stele root rot, Phytophthora fragariae var. fragariae

    NARCIS (Netherlands)

    Weg, van de W.E.

    1997-01-01

    Red stele (red core) root rot is the major soil-borne disease of strawberries <em>(Fragaria> spp.) in many areas with cool, moist soil conditions. It is caused by the soil-borne fungus <em>Phytophthora fragariaeem> var. <em>fragariae.> Red stele

  2. Fungos micorrízicos arbusculares em bananeiras cultivadas no Vale do Submédio São Francisco Arbuscular mycorrhizal fungi associated with roots of banana cultivated in the Submédio São Francisco Valley

    Directory of Open Access Journals (Sweden)

    Adriana Mayumi Yano de Melo

    1997-12-01

    Full Text Available Na região do Vale do São Francisco, onde a exploração da agricultura irrigada é a principal responsável pela alta produção de frutas para exportação, a banana é uma das culturas que mais tem se expandido em área cultivada. Com o objetivo de registrar os fungos micorrízicos arbusculares associados a essa cultura, foram coletadas, sob a parte aérea da bananeira, amostras de solo da rizosfera, em quatro áreas com diferentes características de solo, sendo identificadas as espécies: Acaulospora longula Spain & Schenck, A. morrowiae Spain & Schenck, A. rehmii Sieverding & Toro, A. scrobiculata Trappe, A. spinosa Walker & Trappe, A. tuberculata Janos & Trappe, Entrophospora infrequens (Hall Ames & Schneider, Gigaspora ramisporophora Spain, Sieverding & Schenck, Glomus diaphanum Morton & Walker, G. etunicatum Becker & Gerd., G. intraradices Schenck & Smith, G. macrocarpum Tui. & Tui., G. mosseae (Nicol. & Gerd. Gerd. & Trappe, G. occultum Walker e Scutellospora sp. A percentagem de colonização das raízes de bananeiras nas diferentes áreas foi em média 55%.In the region of the São Francisco Valley, where irrigated agriculture is responsable for high production of fruits for exportation, banana is one of the most expanded crops in cultivated areas. In order to know the arbuscular mycorrhizal fungi associated with this crop, samples from the rhizosphere of banana plants were collected in four areas with different soil characteristics, being identified the species: Acaulospora longula Spain & Schenck, A. morrowiae Spain & Schenck, A. rehmii Sieverding & Toro, A. scrobiculata Trappe, A. spinosa Walker & Trappe, A. tuberculata Janos & Trappe, Entrophospora infrequens (Hall Ames & Schneider, Gigaspora ramisporophora Spain, Sieverding & Schenck, Glomus diaphanum Morton & Walker, G. etunicatum Becker & Gerd., G. intraradices Schenck & Smith, G. macrocarpum Tui. & Tui., G. mosseae (Nicol. & Gerd. Gerd. & Trappe, G. occultum Walker and

  3. Root disease management guidebook

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-31

    Forest tree root pathogens are widespread throughout all forested ecosystems in British Columbia. This guidebook provides a background to forest root disease management (including why, where, and how to manage root disease) and describes the necessary tools for managing root disease. It includes a review of the distribution of major root diseases in the province, host susceptibility and symptomology, and root disease and stand dynamics. The tools described include disease hazard and risk assessment, stratification surveys, and treatment methods. The major root diseases covered in the guide are Armillaria root disease, laminated root rot, Tomentosus root rot, blackstain root disease, and Annosus root disease.

  4. Distribuição das raízes dos citros em função da profundidade da cova de plantio em Latossolo Amarelo dos Tabuleiros Costeiros Citros root distribution in Coastal Tablelands Yellow Latosol in different planting hole depth

    Directory of Open Access Journals (Sweden)

    Laercio Duarte Souza

    2006-04-01

    Full Text Available A citricultura no Nordeste do Brasil está concentrada nos Estados da Bahia e Sergipe, com 106.385 ha de área plantada no agroecossistema dos Tabuleiros Costeiros, onde predominam Latossolos Amarelos, que apresentam horizontes coesos subsuperficiais que se tornam extremamente duros quando secos. Esses horizontes impedem o desenvolvimento das raízes ao longo do perfil, diminuindo o volume de solo explorado e a disponibilidade de água e nutrientes. Para romper a zona de ocorrência da coesão e aumentar o volume de solo ocupado pelas raízes, foram utilizados plantios com profundidades de cova de 0,40; 0,60; 0,80; 1,00 e 1,20 m, com laranjeira 'Valência' enxertada sobre limoeiro 'Volkameriano'. A distribuição das raízes, divididas em quatro diâmetros, apresentou diferenças entre tratamentos. Os melhores resultados ocorreram na linha de plantio para os tratamentos 0,80 m, 1,00 m e 1,20 m.Citros crop in Northeast Brazil is concentrated on states of Bahia and Sergipe, with 106,385 hectares, established in the Coastal Tablelands agricultural ecosystem, where Yellow Latosol prevail, with cohesive horizons that become hard when dry. This problem retains the roots development along the soil profile, decreasing the soil volume explored and consequently the availability of water and nutrients. To solve this problem, breaking the cohesive layer and increasing the volume of roots in the soil, orange tree 'Valência' grafted on lemon tree 'Volkameriano' was planted in several hole depths (0.40; 0.60; 0.80; 1.00 and 1.20 m. The distribution of roots, evaluated in four different diameters, showed differences among the treatments. The best depths were 0.80 m, 1.00 m and 1.20 m.

  5. Antibacterial effect of root canal preparation and calcium hydroxide paste (Calen intracanal dressing in primary teeth with apical periodontitis Efeito antibacteriano do preparo biomecânico e do curativo de demora com pasta à base de hidróxido de cálcio (Calen em dentes decíduos com lesão periapical

    Directory of Open Access Journals (Sweden)

    Gisele Faria

    2005-12-01

    Full Text Available The aim of this study was to evaluate the antibacterial action of root canal mechanical preparation using 2.5% sodium hypochlorite as the irrigating solution and a calcium hydroxide paste as the antibacterial intracanal dressing in human primary teeth root canals with pulp necrosis and apical periodontitis by means of microbial culture. A total of 26 root canals of human primary teeth with pulp necrosis and apical periodontitis were used. Samples were collected before, 72h after biomechanical treatment and 72h after removal of the intracanal dressing. Comparison by Wilcoxon test showed that root canal mechanical preparation effectively eliminated all microorganisms in 20% of the root canals, and the intracanal dressing in 62.5%; however, the cumulative action of biomechanical treatment and intracanal dressing eliminated the microorganisms of 70% of the root canals (pO objetivo do presente estudo foi avaliar, por meio de cultura bacteriológica, a ação antibacteriana do preparo biomecânico utilizando como solução irrigadora o hipoclorito de sódio a 2,5% e da pasta Calen utilizada como curativo de demora em canais radiculares de dentes decíduos de humanos com necrose pulpar e lesão periapical. Foram selecionados 26 dentes decíduos de humanos portadores de necrose pulpar e lesão periapical. As colheitas microbiológicas foram efetuadas antes e 72 horas após o preparo biomecânico e 72 horas após a remoção do curativo de demora. A comparação por meio do teste de Wilcoxon mostrou que o preparo biomecânico foi eficaz na eliminação dos microrganismos dos canais radiculares em 20% dos casos e o curativo de demora em 62,5%, enquanto que a ação cumulativa do preparo biomecânico e do curativo de demora eliminou os microrganismos em 70,0% dos casos (p<0.001. Pôde-se concluir que o preparo biomecânico, isoladamente, apresentou resultados microbiológicos inferiores àqueles obtidos quando o mesmo foi associado ao curativo de demora

  6. Prevalence of microorganisms in root canals of human deciduous teeth with necrotic pulp and chronic periapical lesions Prevalência de microrganismos em canais radiculares de dentes decíduos de humanos com necrose pulpar e lesão periapical crônica

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    Luciana Cunha Pazelli

    2003-12-01

    Full Text Available The objective of this study was to evaluate bacterial prevalence in 31 root canals of human deciduous teeth with necrotic pulp and periapical lesions using bacterial culture. After crown access, the material was collected using absorbent paper points for microbiological evaluation and determination of colony forming units (CFU. Anaerobic microorganisms were found in 96.7% of the samples, black-pigmented bacilli in 35.5%, aerobic microorganisms in 93.5%, streptococci in 96.7%, and S. mutans in 48.4%. We concluded that in human deciduous teeth root canals with necrotic pulp and periapical lesions the infection is polymicrobial, with a large number of microorganisms and a predominance of streptococci and anaerobic microorganisms.O objetivo deste estudo foi avaliar, por meio de cultura bacteriológica, a prevalência de microorganismos em 31 canais radiculares de dentes decíduos humanos com necrose pulpar e lesão periapical. O material, colhido dos canais radiculares após a realização da cirurgia de acesso, foi submetido ao processamento microbiológico para a determinação das unidades formadoras de colônia de microorganismos. Os resultados mostram que os microorganismos anaeróbios foram quantificados em 96,7% dos casos, os bacilos pigmentados de negro (BPB em 35,5%, os aeróbios em 93,5%, os estreptococos em 96,7% e os S. mutans em 48,4%. Assim, pôde-se concluir que a infecção em canais radiculares de dentes decíduos humanos portadores de necrose pulpar e lesão periapical é polimicrobiana, com grande quantidade de microorganismos e maior prevalência de estreptococos e microorganismos anaeróbios.

  7. Comparison of the cleaning efficacy of the FKG race system and hand instrument in molar root canal Comparação da eficácia de limpeza entre o sistema FKG race e instrumentos manuais em molares inferiores

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    Carla Renata Sipert

    2006-01-01

    Full Text Available INTRODUCTION AND OBJECTIVE: The aim of this study was to evaluate the ability of root canal cleaning of the RaCe rotary instrumentation system (FKG Dentaire - La-Cheaux-de Fonds - Switzerland, compared to manual filing with Stainless Steel K-files (Maillefer Instruments - Ballaigues - Switzerland. MATERIAL AND METHOD: Twenty extracted human teeth (maxillary molars were selected and their pulp tissue was removed after coronal access. The root canals were filled with a dye (India ink and allowed to dry for 48 hours, followed by establishment of the working length of the mesiobuccal root; then, half of the specimens were instrumented by the modified Oregon technique and the other half were instrumented by crown-down sequence of the RaCe system. After preparation, the teeth were longitudinally sectioned and evaluated according to the amount of remaining dye. RESULTS: Data obtained were registered as numerical scores, and the arithmetic means were compared between groups using the Mann Whitney test. Both techniques were unable to completely clean the interior of the root canals, with a better performance of the manual technique only at the middle third. CONCLUSION: It could be concluded that the RaCe system was able to provide satisfactory cleaning, similar to that obtained by the manual instrumentation technique.Objetivo deste trabalho foi avaliar a capacidade de limpeza de canais radiculares por meio do sistema de instrumentação RaCe (FKG Dentaire - La-Cheaux-de Fonds - Suíça em comparação à instrumentação manual por meio de limas tipo K-File (Maillefer Instruments - Ballaigues - Suíça. Foram selecionados 20 dentes humanos extraídos (molares superiores que tiveram seu conteúdo radicular removido após realização da abertura coronária. Os canais foram preenchidos com corante (tinta nanquim e após 48 horas para secagem, realizou-se a odontometria da raiz mésio-vestibular. Metade dos espécimes foi instrumentada pela técnica de

  8. Locally Finite Root Supersystems

    OpenAIRE

    Yousofzadeh, Malihe

    2013-01-01

    We introduce the notion of locally finite root supersystems as a generalization of both locally finite root systems and generalized root systems. We classify irreducible locally finite root supersystems.

  9. Crescimento radicular, extração de nutrientes e produção de grãos de genótipos de milho em diferentes quantidades de palha de aveia-preta em plantio direto Root growth, nutrient extraction and grain yield of corn genotypes under different amounts of black oat crop residues under no-tillage

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    João Carlos de Moraes Sá

    2010-08-01

    Full Text Available A manutenção da palha na superfície do solo atua principalmente na proteção contra o impacto das gotas de chuva, reduzindo a desagregação, o escorrimento superficial, o transporte de sedimentos e, consequentemente, a erosão. Essa proteção pode ter efeitos significativos nos atributos da planta de milho. O objetivo deste trabalho foi avaliar o efeito da quantidade de palha de aveia-preta em plantio direto no crescimento radicular, na extração de nutrientes e na produção de grãos de genótipos de milho. O experimento foi conduzido em um Latossolo Vermelho distrófico típico, em delineamento experimental de blocos ao acaso em parcelas subdivididas, com três repetições. A parcela principal foi representada pela quantidade de palha na superfície do solo (0, sem palha; 5,0; e 10,0 Mg ha-1, e a subparcela, constituída por 13 genótipos de milho. A quantidade de palha alterou significativamente o comprimento radicular e a altura de requeima. Todavia, a interação entre palha e genótipo alterou significativamente as variáveis estudadas. O sistema radicular das plantas de milho foi beneficiado por doses adicionais de palha tanto na camada superficial (0-20 cm como na subsuperficial (50-100 cm do solo. O aumento na quantidade de palha na superfície do solo resultou na maior extração total de N, P e K e não alterou a extração total dos elementos Ca, Mg e S pela planta de milho.Crop residues on the soil surface act mainly as protection against raindrop impact, reducing aggregate disruption, runoff, sediment transport and consequently, erosion. This protection can have significant effects on the corn plant characteristics. The objective of this study was to evaluate the effect of the amount of black oat residues on no-tillage root development, nutrient uptake and grain yield of corn genotypes. The experiment was conducted in an Oxisol, in a randomized block design in split plots with three replications. The main plot was

  10. Enraizamento de estacas de Psidium guajava L. 'Século XXI' tratadas com ácido indolbutírico veiculado em talco e álcool Cutting rooting of Psidium guajava L. 'Século XXI' guava treated with indolebutyric acid with talc and alcohol as a vehicle

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    Lilian Yukari Yamamoto

    2010-05-01

    Full Text Available Na produção comercial de Psidium guajava L. (goiabeira, a qualidade das mudas é de fundamental importância, a fim de garantir a homogeneidade, a rápida formação, a alta produtividade e o início precoce da produção, tornando-se imprescindível a utilização da propagação assexuada. O objetivo deste trabalho foi avaliar o enraizamento de estacas de Psidium guajava L. 'Século XXI' tratadas com diferentes concentrações de ácido indolbutírico (AIB veiculados em talco e álcool. Foram utilizadas estacas herbáceas de 10 - 12cm, submetidas a duas formas de aplicação (solução hidroalcóolica e talco e três concentrações (0; 1000 e 2000mg L-1 de AIB. As estacas foram enraizadas em caixas plásticas contendo casca de arroz carbonizada. O delineamento experimental foi o inteiramente casualizado, com cinco repetições, em um fatorial 2x3, sendo cinco repetições de 10 estacas. Após 95 dias, foram avaliadas as variáveis: sobrevivência das estacas (%; retenção foliar (%; estacas enraizadas (%; número de raízes por estaca; comprimento de raízes por estaca (cm e massa seca das raízes por estaca (g. Os melhores resultados para a porcentagem de estacas enraizadas (28,5%; número de raízes por estaca (12,10 e comprimento da raiz (6,79cm foram obtidos com a maior concentração de AIB. A partir dos resultados obtidos, conclui-se que a aplicação de 2000mg L-1 de AIB proporcionou as melhores características de enraizamento em estacas herbáceas da goiabeira 'Século XXI' e a sua veiculação em talco se mostrou mais eficiente que em álcool.In commercial production of Psidium guajava L. (guava seedlings quality is of paramount importance in order to ensure uniformity, rapid formation, high productivity and early production, making it essential to vegetative propagation. The aim of this research was to evaluate the cutting rooting of Psidium guajava L. 'Século XXI' treated with different concentrations of indolebutyric acid

  11. Enxertia de maracujazeiro-azedo em estacas herbáceas enraizadas de espécies de passifloras nativas Grafting of passion fruit on rooted-herbaceous cuttings of wild passiflora species

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    Renata da Costa Chaves

    2004-04-01

    Full Text Available As doenças provocadas por patógenos do solo em maracujazeiro constituem-se em um dos principais problemas para essa cultura no Brasil. Uma das alternativas de controle dessas doenças seria a utilização de porta-enxertos resistentes. Várias espécies de passifloras nativas vêm apresentando resistência a essas doenças, mas a utilização destas como porta-enxertos oriundos de sementes tem sido dificultada pelas diferenças de diâmetro entre o porta-enxerto e o enxerto da espécie comercial, o que não aconteceria caso fossem utilizadas as estacas herbáceas como porta-enxerto. No presente experimento, utilizaram-se estacas herbáceas retiradas da parte mediana de ramos de plantas de Passiflora setacea (acesso EC-PS 1, P. nitida (acesso EC-PN 1, P. caerulea (acesso EC-PC 1, P. actinia (acesso EC-PA 1 e de um híbrido F1 entre P. setacea x P. edulis f. flavicarpa comercial e tratadas com ácido naftaleno acético (ANA a 500 mg/L e mantidas em câmaras de nebulização. As enxertias do tipo "garfagem lateral no topo" foram efetuadas aos 40; 55 e 70 dias após a coleta e plantio das estacas, utilizando garfos de uma única planta de maracujazeiro-azedo. As avaliações foram efetuadas aos 145 e 150 dias após o plantio das estacas, determinando-se a porcentagem de pegamento da enxertia e de enxertos brotados e o comprimento do broto do enxerto. A produção de mudas por enxertia em estacas herbáceas enraizadas de Passiflora nitida e do híbrido F1 (P. setacea x P. edulis f. flavicarpa foi tecnicamente viável.The soil born diseases cause expressive losses in passion fruit crops in Brazil. The use of resistant rootstocks is an alternative to control these diseases. Several wild species of Passifloraceae have presented resistance to soil born pathogens, but their utilization as rootstock from seeds has been limited mainly by differences of thickness between the rootstock and the graft of the commercial cultivars. These limitations could be

  12. Enraizamento de estacas lenhosas de pessegueiro cv. Okinawa em diferentes diâmetros de ramos, substratos e recipientes Rooting of peach cv. Okinawa hardwood cuttings at different stem diameters, substrates, and pots

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    Mauro Brasil Dias Tofanelli

    2003-06-01

    Full Text Available O trabalho foi desenvolvido no Departamento de Produção Vegetal - Setor Horticultura da Faculdade de Ciências Agronômicas (FCA da Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP, Campus de Botucatu (SP com o objetivo de avaliar a influência de diferentes substratos e tipos de recipientes no potencial de enraizamento de estacas lenhosas de pessegueiro cultivar Okinawa com diferentes diâmetros. As estacas foram tratadas com 2,5g L-1 de ácido indol-butírico. O período de permanência das estacas na casa de vegetação foi de 50 dias. Os tratamentos consistiram de seis substratos: areia, casca de arroz carbonizada, vermiculita, areia + casca de arroz carbonizada, areia + vermiculita e casca de arroz carbonizada + vermiculita, com as misturas na proporção 1:1v/v, três tipos de recipientes: sacos plásticos, bandejas de poliestireno expandido e bandejas plásticas e dois grupos de estacas com diâmetros diferentes: 2 a 6mm e 6 a 10mm. O melhor resultado de enraizamento foi obtido em sacos plásticos com vermiculita independente do diâmetro das estacas.This work was carried out at the Department of Plant Production/Horticulture of the Faculdade de Ciências Agronômicas (FCA of the Universidade Estadual Paulista (UNESP located in Botucatu (SP, Brazil. The purpose of this study was to evaluate the influence of substrate and pot type on rooting of stem hardwood cuttings of peach cultivar Okinawa with different diameters. The cuttings were treated with 2.5g L-1 of IBA. The cuttings were maintained in greenhouse for 50 days. The treatments consisted of the combination of six substrate compositions: sand, carbonized rice husk, vermiculite, sand + carbonized rice husk, sand + vermiculite, and carbonized rice husk + vermiculite, mixture at proportion 1:1v/v and three types of pots: plastic bags, polystyrene trays, plastic trays and two groups of cutting diameter: 2 to 6mm and 6 to 10mm. The highest rooting frequency was obtained

  13. Emissão de folhas e início de acumulação de amido em raízes de uma variedade de mandioca em função da época de plantio Leaf emergence and beginning of starch accumulation in roots of a cassava variety as a function of planting date

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    Alfredo Schons

    2007-12-01

    Full Text Available O número de folhas acumulado (NF na haste principal está associado com o aparecimento de vários estádios de desenvolvimento da cultura da mandioca. O início de acumulação de amido (IAA nas raízes tuberosas da mandioca marca o início da translocação dos fotoassimilados para o principal órgão de reserva nessa espécie. O objetivo deste trabalho foi estimar o filocrono e identificar um indicador morfológico baseado no NF para o IAA em mandioca plantada em diferentes épocas. Um experimento a campo foi conduzido em Santa Maria, RS, Brasil, com quatro épocas de plantio (28/09; 11/10; 16/11 e 27/12/2005. A variedade de mandioca usada foi a "RS 13", plantada em baldes de 12 litros, enterrados no espaçamento com 2,0 x 0,8m. O delineamento experimental foi o inteiramente casualizado com 11 repetições. A soma térmica diária (STd, °C dia foi calculada a partir da emergência e acumulada por: STa = sigmaSTd. O IAA foi determinado nas plantas de cada balde quando uma raiz atingia um diâmetro de 1cm. O NF na data do IAA e o número final de folhas (NFF até o primeiro simpódio também foram determinados. O filocrono variou de 20,3 a 29,5°C dia folha-1 e seus valores foram maiores quanto mais tardio foi o plantio. O NFF variou entre as épocas de plantio, aumentando com o atraso do plantio. O NF no IAA foi similar nas quatro épocas de plantio e ocorreu quando, em média, o NF corresponde a 20,9 (±1,0 folhas.The accumulated number of leaves (NF on the main stem is related to the appearance of several developmental stages in cassava. The beginning of starch accumulation (IAA in the fibrous roots of cassava switches the source/sink ratio because of the translocation of photoassimilates to the major storage organ in this species. This study was aimed at estimating the phyllochron and identifing a morphological indicator based on NF for IAA in cassava grown in several planting dates. A field experiment was carried out in Santa Maria, RS

  14. Enraizamento de estacas do porta-enxerto de marmeleiro (Cydonia oblonga Mill. cv. EMC, em diferentes substratos, concentrações de ácido indolbutírico e enxertia de raiz Effect of substrate, indolebutyric acid and root grafting on the propagation of quince (Cydonia oblonga MILL. cultivar emc by cuttings

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    Clevison Luiz Giacobbo

    2007-02-01

    Full Text Available Com este trabalho, objetivou-se testar técnicas para o enraizamento de microestacas lenhosas de marmeleiro cv. EMC por meio de diferentes substratos, concentrações de ácido indolbutírico (AIB e a enxertia de raiz. Foram realizados dois experimentos; no experimento 1 testou-se concentrações de AIB (0, 1000, 2000 e 3000 mg.L-1 e no experimento 2 enxertia de raiz (com e sem enxertia de raiz. Para ambos os experimentos as estacas foram acondicionadas em três substratos (vermiculita, Plantmaxâ e vermiculita + Plantmaxâ, em proporção de volume de 1:1 e avaliado o número e comprimento de raízes e brotos por estacas, percentual de estacas enraizadas e, percentual de sobrevivência a campo. No experimento 1, verificou-se resposta linear positiva para número médio de raízes e negativo para número e comprimento de brotações e sobrevivência a campo, enquanto que nas variáveis comprimento médio de raízes e porcentagem de enraizamento, observou-se comportamento polinomial quadrático, com melhor resposta obtida com 1354 e 2119 mg.L-1 de AIB, respectivamente. No experimento 2, verificou-se que a utilização de vermiculita + Plantmaxâ apresentou maior número de raízes por estaca, quando utilizada enxertia de raiz, porém menor comprimento de brotos. A campo, a melhor resposta foi obtida nas plantas sem enxertia de raiz e oriundas do substrato vermiculita, 93,75%.The objective for this study was to evaluate the effects of different substrates, concentrations of indolebutyric acid (IBA, and root grafting on the rooting of quince (Cydonia oblonga Mill. cv. EMC hardwood cuttings. Two experiments were carried out. In the experiment 1, four concentrations of IBA (0; 1000; 2000 and 3000 mg.L-1 were tested. In the experiment 2, cuttings with or without root grafting were tested. In both experiments the cuttings were placed in one of the three substrates: vermiculite; Plantmax® ; or Plantmax® + vermiculite, 1:1 (v/v. The variables evaluated

  15. Efficacy of three conditions of radiographic interpretation for assessment root canal length Eficácia de três condições de interpretação radiográfica em odontometria

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    Mitsuru Ogata

    2005-03-01

    Full Text Available OBJECTIVE: To compare the efficacy of three conditions of image interpretation for radiographic root measurements and calculating the intra-observer reproducibility of the measurements. MATERIAL AND METHODS: Thirty intra-operative periapical radiographs of maxillary central and lateral incisors were measured, in mm, from the tip of the file to the radiographic apex, using a caliper. Three separate measurements were made of the 30 radiographs. The three measurements for each tooth were averaged and the mean used for further calculations. After a 12-day period, the measurements were repeated. The three experimental viewing conditions used: 1 standard viewbox without masking of background light around the radiograph and without magnification (Visual; 2 standard viewbox with use of a magnifying lens of 2.5x and with background light masked (Magnification; and 3 viewer device that restricts room lighting and enlarges the image by a magnifying lens of 1.75x (Viewer. The mean and standard deviation of the measurements were calculated and used for descriptive analysis. Two-way analysis of variance (ANOVA was used to evaluate intra-observer and inter-method agreement of the measurements. The measurement error was estimated by Dalhberg's formula. RESULTS: The ANOVA showed no significant differences between measurement sessions, viewing methods, or interaction between observation session and method (p>0.05. The intra-observer measurement error was 0.02 mm for Visual and the Magnification methods and 0.01 mm for the Viewer. CONCLUSION: There does not seem to be any advantage in using viewbox masking or magnification for measuring the distance between the end of the endodontic file and the root apex in maxillary incisors.OBJETIVO: Comparar a eficácia de três condições de interpretação radiográfica em odontometria e avaliar a concordância intra-observador. MATERIAL E MÉTODO: Trinta radiografias periapicais de incisivos central e lateral superiores

  16. Enraizamento de estacas herbáceas dos porta-enxertos de videira ‘Campinas’ (IAC 766 e ‘Jales’ (IAC 572 em diferentes substratos Herbaceous cutting rooting of ‘Campinas’ (IAC 766 and ‘Jales’ (IAC 572 vine roostocks on different growth medium

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    Sérgio Ruffo Roberto

    2004-10-01

    Full Text Available O presente trabalho teve por objetivo avaliar o enraizamento de estacas herbáceas de dois porta-enxertos de videiras (‘Campinas’ - IAC 766 e ‘Jales’ - IAC 572 em três tipos de substratos (casca de arroz carbonizada, vermiculita de grânulos finos e vermiculita de grânulos médios. Para tanto, estacas retiradas de plantas matrizes livres de vírus foram preparadas a partir das porções medianas de ramos tenros e verdes. O enraizamento foi realizado em caixas plásticas perfuradas contendo os substratos em câmara de nebulização. O delineamento experimental empregado foi o inteiramento casualizado com 6 tratamentos e 5 repetições, sendo cada parcela composta por 10 estacas. Após quatro semanas da estaquia, o efeito dos fatores (porta-enxertos e substratos foi avaliado, e se pode concluir que: a a porcentagem média de enraizamento dos porta-enxertos nos diferentes substratos é alta (90%; b a casca de arroz carbonizada propicia os melhores resultados de enraizamento para os porta-enxertos estudados; e c o ‘Campinas’ é superior em relação ao ‘Jales’ quanto ao número de raízes emitidas por estaca e à matéria do seu sistema radicular.This research was an attempt to evaluate the herbaceous cutting rooting of two vine rootstocks (‘Campinas’ - IAC 766 and ‘Jales’ - IAC 572 on three types of plant growth medium (hull rice coal, vermiculite - small granules and vermiculite - medium granules. Cuttings were taken from a virus-free stock plant, through the removal of green branches. Firstly, preparation of cuttings consisted on the elimination of the leaves of the basal portion, leaving one leaf in the superior part of each one. After that, the cuttings were placed in plastic boxes containing different types of growth medium on mist chamber. A randomized design was used as statistical model with 6 treatments and 5 replications. Each plot was composed of 10 cuttings. Once the proper rooting was observed, the effect of

  17. Distribuição de raízes de laranja "Pêra" sob sequeiro e irrigação por microaspersão em solo arenoso Root distribution of "Pêra" orange trees under non-irrigated and irrigated conditions in a sandy soil

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    Eugênio Ferreira Coelho

    2002-05-01

    Full Text Available O trabalho teve por objetivo avaliar a distribuição de raízes de laranja "Pêra" sob condições não-irrigadas e irrigadas por microaspersão em solo arenoso de tabuleiro costeiro. As raízes foram extraídas em trincheiras, a partir do tronco, nas direções longitudinal e ortogonal à fileira de plantas, pelo método do monolito. Uma vez separadas, foram digitalizadas com uso de computador e scanner, para obter, com o uso do software Rootedge, os comprimentos e diâmetros dos segmentos de raízes de todas as amostras, que foram mapeados nos perfis amostrados. O sistema radicular sob irrigação por microaspersão apresentou maior expansão, tanto em profundidade como em distância radial do tronco, do que o sistema radicular sob condições não-irrigadas. Houve maior porcentagem de raízes finas nos perfis de solo sob microaspersão, em relação à condição não-irrigada, indicando a possibilidade de maior atividade do sistema radicular nesse sistema de irrigação. As posições mais adequadas para instalação de sensores de água do solo para a cultura da laranja sob microaspersão estão entre 0 e 2,5 m de distância radial a partir do tronco, em profundidades entre 0 e 1,0 m.The objective of this work was to evaluate root distribution of orange cv. Pêra under non-irrigated and under microsprinkler irrigated conditions in a Coastal Plain Sandy Soil. Root samples were collected by monolith method from trenches, which were dug aside, the plant trunk at longitudinal and orthogonal directions to plant rows. After separation from soil, roots were scanned and digitized in a PC computer. Root length and diameter were determined using Rootedge software that allowed mapping root distribution on sampled soil profiles. Results showed that root system of microsprinkler irrigated trees occupied higher volume in the soil compared to the one under non-irrigated conditions. Higher amounts of fine roots were found in the profiles of microsprinkler

  18. Influência da posição da estaca no ramo sobre o enraizamento de Pfaffia glomerata (Spreng. Pedersen em dois substratos Shoot cutting position effect on rooting of Pfaffia glomerata (Spreng. Pedersen in two substrates

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    Fernando Teixeira Nicoloso

    1999-06-01

    Full Text Available A fáfia (Pfaffia glomerata (Spreng. Pedersen é uma espécie que vem sendo utilizada na medicina popular contra várias enfermidades, tais como inflamações e reumatismo, bem como para combater fadiga e estresse. Por isso, os recursos naturais dessa espécie têm sido consideravelmente explorados e informações sobre métodos para sua propagação são necessárias. O objetivo deste trabalho foi avaliar a viabilidade da propagação vegetativa via estaquia, a influência da posição da estaca no ramo e do substrato no processo de enraizamento de estacas de fáfia. Os tratamentos consistiram de uma combinação bifatorial (4 x 2, representados por quatro posições das estacas no ramo (basal, mediana-basal, mediana-apical e apical e dois substratos (areia média + casca de arroz carbonizada e solo + casca de arroz carbonizada, 1:1 v/v. As estacas, de 15 a 20cm de comprimento, foram obtidas a partir de cortes sucessivos da base do ramo para o ápice. O delineamento experimental foi inteiramente cazualisado com cinco repetições, sendo a parcela experimental constituída de 20 estacas. O experimento foi instalado em 17 de Julho de 1997 e teve duração de 90 dias, sob condição de temperatura ambiente. Os resultados indicaram que: a estaquia é um método adequado para a propagação da fáfia, estacas obtidas das porções medianas e basal dos ramos apresentam maior capacidade de enraizamento que as apicais e a combinação de solo + casca de arroz carbonizada é um substrato viável para a estaquia e para o posterior desenvolvimento das mudas a campo.Pfaffia glomerata (Spreng. Pedersen is a medicinal herb that has been used in the Brazilian folk medicine against several illnesses, such as inflammations and reumatism. It has also been playing the role of a ginseng-like tonic herb, helping to fight fatigue and combat stress. Therefore, wild resources have been exploited considerably and informations about suitable methods for its propagation

  19. Genome-Wide Selection for tropical maize root traits under conditions of nitrogen and phosphorus stress=Seleção Genômica Ampla para caracteres de raiz em milho tropical em condições de estresse de nitrogênio e fósforo

    Directory of Open Access Journals (Sweden)

    Marcos Deon Vilela de Resende

    2012-10-01

    Full Text Available The objective of this study was to verify the accuracy of the Genome-Wide Selection (GWS method in tropical maize breeding for root traits under conditions of nitrogen and phosphorus stress. Forty-one single-crosses were evaluated in two experiments. The first experiment considered low nitrogen availability, and the second experiment considered low phosphorus availability. A randomized block design with two replicates was used. The lateral and axial root lengths were measured using WinRhizo software. The analysis of deviance was calculated using the Restricted Maximum Likelihood/Best Linear Unbiased Prediction (REML/BLUP method. Eighty microsatellite markers were used to genotype the estimation population. The Random Regression method was used to analyze the GWS (RR-BLUP/GWS data. The gains per unit time of the GWS and the phenotypic selection method were compared, as the standard phenotypic selection methods were considered to be the Recurrent Selection. The GWS accuracy was higher than the phenotypic selection accuracy for all of the traits evaluated. Thus, the GWS method may significantly increase the genetic gains for root traits that are obtained in tropical maize breeding programs for nutritional stress conditions.O objetivo deste estudo foi verificar a acurácia do método da Seleção Genômica Ampla (GWS no melhoramento de milho tropical para caracteres de raiz em condições de estresse de nitrogênio e fósforo. Quarenta e um híbridos simples foram avaliados em dois experimentos. O primeiro experimento considerou baixa disponibilidade de nitrogênio e o segundo baixa disponibilidade de fósforo. Para isto, foi usado o delineamento de blocos casualizados com duas repetições. Os comprimentos de raiz lateral e axial foram medidos utilizando o software WinRhizo. As análises de deviance foram realizadas pelo método da Máxima Verossimilhança Restrita / Melhor Preditor Linear Não-viesado (REML/BLUP. Oitenta marcadores microssat

  20. Produtividade de raízes de mandioca consorciada com milho e caupi em sistema orgânico Yield of cassava roots intercropped with corn and cowpea in an organic system

    Directory of Open Access Journals (Sweden)

    Antonio Carlos Pries Devide

    2009-01-01

    Full Text Available Foram avaliados sistemas orgânicos de produção de mandioca "de mesa", em Seropédica (RJ. O experimento constou dos seguintes tratamentos: monocultivo de mandioca (cv. IAC 576-70 e consórcios com milho experimental (cv. Eldorado, caupi (cv. Mauá e milho+caupi. O manejo orgânico foi padronizado e toda a área experimental irrigada durante o período de permanência do milho no sistema. Do milho, foram colhidas espigas verdes (imaturas e a parte aérea acamada na superfície do solo. O caupi foi incluído como adubo verde e cortado na floração, sendo mantidos os resíduos na superfície do solo. Ambos os consortes ocuparam as entrelinhas da mandioca, de modo alternado, semeados após a primeira capina da cultura principal. A cultivar IAC 576-70 mostrou-se adaptada ao manejo orgânico, com produtividade de raízes de padrão comercial próxima a 31 Mg ha-1. Não houve diferenças significativas entre o monocultivo e os três tipos de consórcios testados. A inclusão do milho representou potencial de renda adicional ao produtor, colhendo-se, em média, 18.125 espigas ha-1, o que correspondeu a 5,1 Mg ha-1. Os resíduos provenientes da roçada do caupi proporcionaram um aporte de biomassa fresca de 12 Mg ha-1, com uma expressiva contribuição em nitrogênio (cerca de 44 kg de N ha-1. A fabácea (leguminosa cobriu por completo as entrelinhas da mandioca, demonstrando seu potencial de controle à erosão e a ervas espontâneas. O consórcio triplo mostrou-se vantajoso tendo em vista que a receita obtida com a venda do milho verde justificaria os custos da irrigação, além dos benefícios da inclusão do caupi e da não interferência dos consortes na produtividade da mandioca.Organic systems were evaluated for cassava root production directed to human comsumption in natura, at Seropédica-RJ. Treatments consisted of: cassava ('IAC 576-70' in single cropping and its intercropping with corn ('Eldorado', cowpea ('Mauá' or corn plus cowpea

  1. Trapping of root-knot nematodes by the adhesive hyphae-forming fungus Arthrobotrys oligospora

    NARCIS (Netherlands)

    Belder, den E.

    1994-01-01

    The present study addresses the ecology of a particular isolate of <em>Arthrobotrys oligosporaem> (CBS 289.82) in relation to its efficacy in controlling the root-knot nematode, <em>Meloidogyne hapla.em>

    This isolate was selected because it differs from

  2. Armillaria root rot of tea in Kenya : characterization of the pathogen and approaches to disease management

    NARCIS (Netherlands)

    Otieno, W.

    2002-01-01

    The rare occurrence of basidiomata and rhizomorphs constrains diagnosis of Armillaria root rot and identification of <em>Armillaria> species in Africa. This has had a negative impact on taxonomic research on the genus <em>Armillaria> in the continent, where the

  3. Genotoxicity of <em>Euphorbia hirtaem>: An <em>Allium cepaem> Assay

    Directory of Open Access Journals (Sweden)

    Kwan Yuet Ping

    2012-06-01

    Full Text Available The potential genotoxic effects of methanolic extracts of <em>Euphorbia hirta em>which is commonly used in traditional medicine to treat a variety of diseased conditions including asthma, coughs, diarrhea and dysentery was investigated using <em>Allium cepaem> assay. The extracts of 125, 250, 500 and 1,000 µg/mL were tested on root meristems of <em>A. cepaem>. Ethylmethanesulfonate was used as positive control and distilled water was used as negative control. The result showed that mitotic index decreased as the concentrations of <em>E. hirtaem> extract increased. A dose-dependent increase of chromosome aberrations was also observed. Abnormalities scored were stickiness, c-mitosis, bridges and vagrant chromosomes. Micronucleated cells were also observed at interphase. Result of this study confirmed that the methanol extracts of <em>E. hirta em>exerted significant genotoxic and mitodepressive effects at 1,000 µg/mL.

  4. Seedling root targets

    Science.gov (United States)

    Diane L. Haase

    2011-01-01

    Roots are critical to seedling performance after outplanting. Although root quality is not as quick and simple to measure as shoot quality, target root characteristics should be included in any seedling quality assessment program. This paper provides a brief review of root characteristics most commonly targeted for operational seedling production. These are: root mass...

  5. Prevalência de cárie radicular em adultos e idosos na região sudeste do Estado de São Paulo, Brasil Dental root surface caries prevalence among adults and senior citizens in southeast São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    Lilian Berta Rihs

    2005-02-01

    Full Text Available Observa-se uma ocorrência maior de cárie radicular nas últimas décadas, sendo este tipo de lesão mais comum em adultos e idosos. Em virtude disso, o objetivo deste estudo foi avaliar a prevalência de cáries radiculares em adultos e idosos da região Sudeste do Estado de São Paulo, Brasil. Exames epidemiológicos foram realizados seguindo recomendações da OMS, sendo examinadas 1.475 pessoas dentadas, dos grupos etários de 35 a 44 e de 65 a 74 anos. A prevalência de cáries radiculares foi de 15,6% e 31,8%, respectivamente, para 35 a 44 e 65 a 74 anos. A média de raízes cariadas e/ou restauradas foi de 0,36 para os adultos, maior entre os homens (p In recent decades there has been an increase in the occurrence of dental root surface caries, especially among adults and senior citizens. This study aimed to identify root surface caries prevalence in adults and seniors from southeast São Paulo State, Brazil. An epidemiological survey was carried out among 1,475 dentate adults, ages 35 to 44 and 65 to 74, respectively, according to WHO criteria. Root surface caries prevalence was 15.6% and 31.8%, for the 35-44 and 65-74-year groups, respectively. The average rate for decayed and/or filled roots was 0.36 for the middle-aged adult group and higher in males (p < 0.01, as compared to 0.82 for seniors, with no difference by gender. The average rate of decayed roots was 0.18 and 0.53 for middle-aged adults and seniors, respectively; in both groups the levels were higher among men (p < 0.01. Further studies on oral health, mainly on root surface conditions, are essential in both middle-aged adults and senior citizens.

  6. Densidade, porosidade e resistência à penetração em Latossolo cultivado sob diferentes sistemas de preparo do solo Soil bulk density, porosity and resistance to root penetration in an Oxisol managed by different soil tillage systems

    Directory of Open Access Journals (Sweden)

    Cássio Antonio Tormena

    2002-12-01

    Full Text Available Os sistemas de preparo e manejo do solo determinam as condições físicas para o crescimento das plantas e produtividade das culturas. O objetivo deste estudo é o de avaliar algumas propriedades físicas de um Latossolo Vermelho distrófico, cultivado com mandioca, após dois anos de implantação em diferentes sistemas de preparo do solo. Os tratamentos utilizados foram: Plantio Direto (mobilização do solo somente ao longo das linhas de plantio; Preparo Mínimo (escarificação a 0,30 m, seguido de gradagem niveladora e Preparo Convencional (aração, com arado de aiveca a uma profundidade de 0,25 m, seguido de gradagem niveladora. Avaliaram-se, nas camadas de 0-0,10 m e 0,10-0,20 m, as seguintes propriedades físicas do solo: densidade, volume de macroporos, microporos, a porosidade e a resistência do solo à penetração das raízes, a cada 0,05 m, até à profundidade de 0,40 m, em três diferentes épocas. Constataram-se maiores valores de densidade do solo e menores valores de macroporosidade na camada de 0-0,10 m no plantio direto e preparo mínimo do solo. Resultados similares foram obtidos na avaliação da resistência do solo à penetração, independente da época de avaliação e da umidade do solo. Os sistemas de preparo mínimo e preparo convencional do solo proporcionam condições físicas menos restritivas ao crescimento das plantas, quando comparado com o plantio direto. Avaliações de médio-longo prazo são necessárias face às mudanças dinâmicas na qualidade física do solo, impostas pelos sistemas de preparo e manejo do solo e da cultura.Soil tillage and management affect soil physical conditions for plant growth and crop productivity. The objective of this research was to evaluate some soil physical properties of an Typic Haplorthox located in the Northwest region of the State of Paraná, Brazil, after two years of different tillage systems, with same crop. The tillage systems used were: No-tillage (cultivated

  7. Reação a doenças e produtividade de um clone de maracujazeiro-azedo propagado por estaquia e enxertia em estacas herbáceas de Passiflora silvestre Reaction to diseases and yield of a passion fruit clone propagated by cuttings and grafting on rooted-herbaceous cuttings of wild Passiflora specie

    Directory of Open Access Journals (Sweden)

    Nilton Tadeu Vilela Junqueira

    2006-04-01

    Full Text Available As doenças causadas por patógenos do solo provocam perdas expressivas em maracujazeiros comerciais. Uma das alternativas de controle dessas doenças seria a utilização de porta-enxertos resistentes. Passiflora nitida é uma das espécies de Passifloraceae que apresentam resistência a essas doenças, mas suas sementes geram porta-enxertos com caules finos e, portanto, incompatíveis com o diâmetro dos garfos da espécie comercial. Este problema pode ser resolvido pela utilização da enxertia em estacas enraizadas ou pela enxertia hipocotiledonar, mas, no Brasil, dados sobre o desenvolvimento e produtividade, em campo, de maracujazeiros enxertados, ainda são escassos. Este trabalho teve como objetivo avaliar a reação a doenças, produtividade e características físicas de frutos de um clone de maracujazeiro-azedo (Passiflora edulis f. flavicarpa comercial propagado por estaquia, enxertia em estacas herbáceas enraizadas de P. nitida (acesso EC-PN 1 e por sementes. As plantas foram cultivadas em um Latossolo Vermelho-Amarelo, onde foram conduzidas em espaldeiras verticais com 1,90 metro em altura, com irrigação por gotejamento. As colheitas foram efetuadas semanalmente, durante 14 meses, e as avaliações das doenças foram efetuadas aos 17; 18 e 19 meses após o plantio. A produtividade das plantas propagadas por estaquia foi o dobro das enxertadas e das propagadas por sementes. Plantas enxertadas e propagadas por estaquia foram menos afetadas pelas doenças.Diseases induced by soil-borne pathogens cause expressive losses in passion fruit crops in Brazil. The use of resistant rootstocks could be an alternative to control these diseases. Passiflora nitida is one of the wild species of Passifloraceae, which have presented resistance to these diseases, but its utilization as seedling rootstock has been limited mainly due to differences of thickness between rootstock and the graft of the commercial cultivars. This problem can be solved by

  8. Enraizamento de estacas herbáceas de quatro clones de umezeiro (Prunus mume Sieb. et Zucc. durante o inverno ameno, em Jaboticabal-SP Rooting of herbaceous cuttings of four mume clones (Prunus mume Sieb. et Zucc. during soft winter, in Jaboticabal, São Paulo state, Brazil

    Directory of Open Access Journals (Sweden)

    Newton Alex Mayer

    2003-12-01

    Full Text Available Estudos realizados no Brasil com o umezeiro (Prunus mume Sieb. et Zucc. relatam promissoras perspectivas de utilização desta espécie como porta-enxerto para pessegueiro e nectarineira, em função de sua rusticidade, adaptação ao inverno brando, compatibilidade com Prunus persica, redução do vigor das plantas e melhoria da qualidade dos frutos. Entretanto, em função da propagação por sementes, tem sido observadas diferenças de vigor entre as plantas, resultando em pomares muito heterogêneos. Assim, o presente estudo teve por objetivo estudar o enraizamento de estacas herbáceas de quatro clones de umezeiro (Clones 02, 05, 10 e 15 durante o inverno ameno, em Jaboticabal-SP. O experimento foi conduzido entre os meses de junho e agosto, sendo avaliado aos 70 dias após a estaquia. Pelos resultados obtidos, foi possível concluir que é viável a propagação dos clones estudados por enraizamento de estacas herbáceas durante o inverno. Foram observadas diferenças entre os clones quanto à porcentagem de enraizamento, porcentagem de estacas com calo, número e comprimento das raízes. No conjunto das variáveis analisadas, os melhores resultados foram obtidos com os Clones 10 e 15.Studies developed in Brazil with the mume (Prunus mume Sieb. et Zucc. have shown promising perspectives of using this species as rootstock for peach and nectarine trees, in function of its rusticity, adaptation to soft winter, compatibility with Prunus persica, plant vigor reduction and improvement of the fruit quality. However, due to seed propagation, vigor differences have been observed among plants, resulting in very heterogeneous orchards. Thus, the present study had the objective of studying the rooting of herbaceous cuttings of four mume clones (Clones 02, 05, 10 and 15 in intermittent mist during the soft winter, in Jaboticabal, São Paulo State, Brazil. The experiment was carried out between June and August, being evaluated 70 days after the cutting

  9. Matéria seca e morfologia radicular de cultivares de arroz de terras altas em função da adubação com zinco Dry matter and root morphology of upland rice plants as a function of zinc fertilization

    Directory of Open Access Journals (Sweden)

    Munir Mauad

    2012-06-01

    Full Text Available O objetivo do trabalho foi avaliar a influência da adubação com zinco na matéria seca e morfologia do sistema radicular de cultivares de arroz de terras altas. O experimento foi conduzido em vasos contendo 8 dm³ de Latossolo Vermelho distroférrico, em casa de vegetação, até 64 dias após a emergência das plantas. O delineamento experimental utilizado foi o de blocos casualizados, disposto em arranjo fatorial 4 x 3, quatro níveis de adubação de zinco (0, 4, 8 e 16 mg dm-3 e três cultivares de arroz (‘Caiapó’, ‘Maravilha’ e ‘Primavera’, com quatro repetições. O diâmetro radicular aumentou com o aumento da adubação com Zn. Comprimento, superfície e volume radicular não foram influenciados pelas doses de Zinco. As cultivares do grupo tradicional ‘Caiapó’ e intermediário ‘Primavera’ apresentaram maior comprimento radicular do que a cultivar do grupo moderno ‘Maravilha’. Cultivares com maior comprimento radicular (‘Caiapó’ e ‘Primavera’ exibiram maiores teores de Zn na parte aérea.The study aimed was to evaluate the upland rice cultivars behavior submitted to different levels of zinc, aiming to identify development alterations of the root system and the shoot. The experiment was carried in 8 dm-3 pots in greenhouse, up 64 days after the emergency of the plants. The experiment was design 4 x 3 completely randomized, consisted by four zinc levels (0, 4, 8 and 16 mg dm-3 and three upland rice cultivars (‘Caiapó’, ‘Maravilha’ and ‘Primavera’ with four replications. The root diameter increased by the Zn high level fertilization. Length, surface and root volume did not influenced by the zinc levels. The cultivars of ‘Caiapó’ traditional group and ‘Primavera’ intermediate showed increased root lenght then the ‘Maravilha’ modern group. Cultivars with greater root lenght (‘Caiapó’e ‘Primavera’ demonstrated higher lever of Zn levels in the shoot.

  10. Red, redder, madder : analysis and isolation of anthraquinones from madder roots (Rubia tinctorum)

    NARCIS (Netherlands)

    Derksen, G.C.H.

    2001-01-01

    The roots of <em>Rubia tinctorumem> L. (madder) are the source of a natural dye. The dye components are anthraquinones with alizarin being the main dye component. Alizarin as such is present in madder root in only small quantities, most of the alizarin is present as its

  11. Is there an efficient trap or collection method for sampling Anopheles darlingi and other malaria vectors that can describe the essential parameters affecting transmission dynamics as effectively as human landing catches? - A Review

    Directory of Open Access Journals (Sweden)

    José Bento Pereira Lima

    2014-08-01

    Full Text Available Distribution, abundance, feeding behaviour, host preference, parity status and human-biting and infection rates are among the medical entomological parameters evaluated when determining the vector capacity of mosquito species. To evaluate these parameters, mosquitoes must be collected using an appropriate method. Malaria is primarily transmitted by anthropophilic and synanthropic anophelines. Thus, collection methods must result in the identification of the anthropophilic species and efficiently evaluate the parameters involved in malaria transmission dynamics. Consequently, human landing catches would be the most appropriate method if not for their inherent risk. The choice of alternative anopheline collection methods, such as traps, must consider their effectiveness in reproducing the efficiency of human attraction. Collection methods lure mosquitoes by using a mixture of olfactory, visual and thermal cues. Here, we reviewed, classified and compared the efficiency of anopheline collection methods, with an emphasis on Neotropical anthropophilic species, especially Anopheles darlingi, in distinct malaria epidemiological conditions in Brazil.

  12. Efeito cariostático de restaurações adesivas em superfícies radiculares: estudo in vitro Caries inhibition around adhesive restorations in roots: in vitro study

    Directory of Open Access Journals (Sweden)

    Anderson Takeo HARA

    2000-06-01

    Full Text Available Materiais restauradores que liberam íons flúor e/ou promovem adesão à estrutura dental têm sido relacionados com a inibição do desenvolvimento de lesões de cárie adjacentes às restaurações. A hipótese testada neste estudo foi a de que o uso de resina composta/sistema adesivo tem efeito cariostático semelhante a um material adesivo que libera íons flúor - cimento de ionômero de vidro - sobre a superfície radicular adjacente às restaurações. Foram utilizadas 20 raízes de terceiros molares humanos extraídos, embutidas em resina de poliestireno e planificadas. Cavidades padronizadas foram preparadas e restauradas aleatoriamente com (a Chelon-Fil (Espe ou (b Z100/SingleBond (3M. Valores iniciais (KHNi de microdureza superficial Knoop da dentina foram obtidos a 100, 200 e 300 mim da margem oclusal das restaurações. Uma área de 2,0 mm ao redor da restauração foi delimitada e submetida à indução de cárie artificial. Obtiveram-se, então, os valores finais (KHNf de microdureza, nas mesmas condições e localizações da leitura inicial. As diferenças entre KHNi e KHNf foram consideradas para a análise estatística. As medianas de KHNi - KHNf nas distâncias de 100, 200 e 300 mim foram para (a: -3,8; -0,3; -1,0; e para (b: 3,3; 2,5; 1,7. O teste de Kruskal-Wallis não evidenciou diferença significativa entre as distâncias dentro de cada grupo. Às distâncias de 200 e 300 mim, não houve diferença significativa entre os materiais avaliados. À distância de 100 mim, (a diferiu significativamente de (b (p Dental materials that release fluoride have been shown to be effective in caries inhibition around restorations. Adhesive materials would also be effective in caries inhibition by sealing and protecting cavity margins from acidic demineralization. This in vitro study tested the hypothesis that composite restorations with a dentin adhesive system have a caries preventive effect similar to that of an adhesive material

  13. computer-aided root aided root aided root aided root-locus

    African Journals Online (AJOL)

    User

    equation are the same as the poles of the close loop system. Ideally, a desired performance can be achieved a control system by adjusting the location of roots in the s-plane by varying one or mo system parameters. Root-locus Method is a line. 8023278605. AIDED ROOT. AIDED ROOT-LOCUS NUMERICAL TECHNIQUE.

  14. ROOT Reference Documentation

    CERN Document Server

    Fuakye, Eric Gyabeng

    2017-01-01

    A ROOT Reference Documentation has been implemented to generate all the lists of libraries needed for each ROOT class. Doxygen has no option to generate or add the lists of libraries for each ROOT class. Therefore shell scripting and a basic C++ program was employed to import the lists of libraries needed by each ROOT class.

  15. Enraizamento de estacas de pessegueiro em função do uso de ácido indolbutírico e fertilizante orgânico Rooting of peach cuttings in function of the use of indolbutyric acid and organic fertilizer

    Directory of Open Access Journals (Sweden)

    Ubirajara Ribeiro Mindêllo Neto

    2005-04-01

    Full Text Available Este trabalho teve como objetivo avaliar o efeito de concentrações de ácido indolbutírico e do fertilizante orgânico HFF turfa fértil® no enraizamento de estacas semilenhosas do pessegueiro 'Capdeboscq'. Estacas foram tratadas com AIB em concentrações de 500; 1.000; 2.000 e 4.000 mg.L-1 e água destilada como testemunha (0 mg.L-1. Posteriormente, as estacas foram acondicionadas em bandejas de poliestireno expandido com 72 células, contendo como substrato vermiculita, e colocadas em câmara de nebulização intermitente. Aos 30 dias após o início do experimento, foi colocado o fertilizante orgânico HFF turfa fértil® em concentrações de 0 mL.L-1, 3 mL.L-1 e 5 mL.L-1 nas células onde estavam contidas as estacas. Após 86 dias do início do experimento, foram avaliados a porcentagem de estacas enraizadas e o número médio de raízes por estaca. A aplicação de HFF turfa fértil® em associação ao ácido indolbutírico promoveu maior enraizamento em relação aos tratamentos sem o uso do fertilizante orgânico. A maior porcentagem de estacas de pessegueiro enraizadas foi obtida com a combinação de 2.000 mg.L-1 de AIB e 5 mL.L-1 de HFF turfa fértil®.This work aim to appraise the effect of indolbutyric acid (IBA and HFF turfa fértil® organic fertilizer concentrations on rooting of semi-hardwood cuttings of peach 'Capdebosq'. The cuttings were treated by IBA at 500; 1,000; 2,000 and 4,000 mg.L-1 and destilled water (0 mg.L-1 as witness. Later on the cuttings were planted in expanded polyestirene trays with 72 cells using vermiculite as substrate and placed inside intermitent mist chamber. At 30 days after the beginning of the experiment was places the HFF turfa fértil® organic fertilizer at concentrations of 0, 3 and 5 mL.L-1 on the tray cells. At 86 days were evaluated the rooted cuttings percentage and the average number of roots per cuttings. The use of HFF turfa fértil® in association with IAB promoted higher rooting

  16. Morphological change study on root surfaces treated with curettes, sonic instruments or Er:YAG laser; Estudo in vitro da alteracao morfologica em superficie radicular tratada com curetas, aparelho ultrasonico ou com laser de Er:YAG

    Energy Technology Data Exchange (ETDEWEB)

    Guimaraes Filho, Arlindo Lopes

    2004-07-01

    Periodontal disease is caused by dental plaque and dental calculus on roots surfaces, specially on cervical areas. As dental plaque is the main cause and dental calculus a secondary one, it is practically impossible to separate one factor to the other one. In order to get periodontal tissue health it is necessary to eliminate dental plaque and calculus from root surfaces. In this sense, Er:YAG laser comes in as an excellent way to control periodontal disease, not only, by removing calculus and dental plaque but also for its bacteria reduction. The aim of this study is to compare, by S.E.M., root surfaces changing when they are treated with curettes and ultrasonic scaling or Er:YAG laser irradiation with two different energy levels of 60 mJ/pulse and 100 mJ/pulse and repetition tax of 10 Hz (in the display). It is also objective of this study to check a possible thermic damage to pulp tissue when the roots surfaces are irradiated with Er:YAG laser. We used for this study, five human dental roots, each one of them were cut into four samples, giving us a total of twenty samples, which were divided in five groups of four samples each one. The control group, we did not indicated any kind of treatment. The first group, the roots samples were scaled and planned with Gracey curettes 5/6 and 7/8. The second group, the roots samples were treated with ultrasonic instruments. The third group was irradiated with Er:YAG laser using 60 mJ/pulse , 10 Hz and energy density of 4 J/cm{sup 2} (approximated). The fourth group was irradiated with Er:YAG laser using 100 mJ/pulse, 10 Hz and energy density of 7 J/cm{sup 2} (approximated). The results analysis showed that roots scaling either with Gracey curettes or with ultrasonic instruments created smear layer covering roots surfaces; roots surfaces irradiated with Er:YAG laser showed few roughness in the third group; roots surfaces irradiated with Er:YAG laser showed no smear layer and the Er:YAG laser irradiation did not bring any

  17. Chemical Composition, Antimicrobial and Antitumor Activities of the Essential Oils and Crude Extracts of<em> Euphorbiaem>> macrorrhizaem>

    Directory of Open Access Journals (Sweden)

    Haji Akber Aisa

    2012-05-01

    Full Text Available The present study aimed to examine the chemical composition and biological activity of essential oils extracted from <em>Euphorbia macrorrhizaem> collected from Northwest China. The major constituents of the essential oils of aerial parts and roots of <em>E. macrorrhizaem> are acorenone B (16.72% and 25.80%, (+-cycloisosativene (14.94% and 12.40%, 3a-hydroxy-5b-androstane (10.62% and 5.52%, copaene (7.37% and 6.29%, l-calamenene (4.13% and 4.65% and β-cedrene (8.40% and 7.98%, respectively. The minor components of them are thymene, γ-terpinene, thymecamphor, α-cedrene, zingiberene, trans-caryophyllene, β-chamigrene, curcumene, pentadecane, (−-α-muurolene, cuparene, γ-cadinene, (<em>Z>-3-heptadecene, 1,3,7,7-tetramethyl-2-oxabicyclo(4.4.0dec-5-en-4-one, hexahydrofarnesyl acetone, γ-elixene and palmitinic acid. The antimicrobial and antitumor activitiy of the <em>E. macrorrhizaem> essential oil against <em>Staphyloccocus aureusem>, <em>Escherichia coliem>, <em>Canidia Albicansem> and Caco-2 cells were evaluated. Among all the tested microorganisms and Caco-2 cells, the essential oils showed the strongest inhibitory effect on <em>Staphyloccocus aureus em>(MIC = 2.8 μg/mL and Caco-2 cell (IC50 = 11.86 μg/mL, whereas no effect on <em>Escherichia coliem> and <em>Candida em>albicans>. The data of this study suggested that the <em>E. macrorrhizaem> essential oils have great potential as a natural medicine for microbial infections and cancers.

  18. Morphometric and microscopic evaluation of the effect of gallium nitrate as a root canal dressing in rat teeth submitted to late replantation Avaliação morfométrica e microscópica do efeito do nitrato de gálio usado como curativo intracanal em dentes de ratos reimplantados tardiamente

    Directory of Open Access Journals (Sweden)

    Graziela Garrido Mori

    2006-12-01

    Full Text Available The purpose of this study was to test a gallium nitrate solution, a resorption inhibitor, employed as a root canal dressing in teeth submitted to late replantation. Thirty maxillary right central incisors of rats were avulsed and kept dry for thirty minutes. The teeth were instrumented and the root surfaces were treated with 1% hypochlorite solution followed by application of 2% sodium fluoride. Thereafter, the teeth were divided into two groups according to the root canal dressing: Group I, solution of gallium nitrate; and Group II, calcium hydroxide paste. The teeth were then replanted in their respective sockets. The animals were killed at 15, 30 and 60 days after replantation and the samples were processed for morphometric and microscopic analysis. The results demonstrated that the gallium nitrate solution and the calcium hydroxide paste limited the root resorption, yet they did not impair its occurrence. It may be concluded that gallium nitrate solution and calcium hydroxide paste demonstrated similar performance.Este trabalho tem como objetivo testar a solução de nitrato de gálio, um inibidor da reabsorção, como curativo intracanal para dentes reimplantados tardiamente. Trinta incisivos centrais superiores direitos de ratos foram avulsionados e mantidos a seco por trinta minutos. Todos os dentes tiveram os canais radiculares instrumentados e a superfície radicular tratada com hipoclorito de sódio a 1%, seguido de fluoreto de sódio a 2%. Após isso, os dentes foram divididos em dois grupos de acordo com a medicação intracanal: no grupo I, foi usada solução de nitrato de gálio e grupo II, pasta de hidróxido de cálcio. Todos os dentes foram, então, reimplantados em seus respectivos alvéolos. Passados 15, 30 e 60 dias do reimplante, os animais foram sacrificados e as peças obtidas, processadas em laboratório para análise microscópica e morfométrica. Os resultados mostraram que a solução de nitrato de gálio e a pasta de

  19. Qualidade de raízes de cenoura em sistemas consorciados com alface sob diferentes densidades populacionais Quality of carrot roots in intercropped systems with lettuce under different planting densities

    OpenAIRE

    Aurélio P. Barros Júnior; Francisco Bezerra Neto; Ebenézer de O. Silva; Maria Zuleide de Negreiros; Eliane Q. de Oliveira; Lindomar Maria da Silveira; Jailma S.S. de Lima; Karidja K.C. de Freitas

    2005-01-01

    O experimento foi realizado de junho a setembro de 2003, em campo da ESAM, com o objetivo de avaliar a qualidade de raízes de cenoura (Brasília) em sistemas consorciados com alface (Tainá) sob diferentes densidades populacionais. O delineamento experimental utilizado foi de blocos casualizados, em esquema fatorial 4 x 4, com três repetições. Os tratamentos foram resultantes da combinação de quatro populações de cenoura [(40%, 60%, 80% e 100% da população recomendada no cultivo solteiro (PRCS)...

  20. Acid phosphatase-1.1, a molecular marker tightly linked to root-knot nematode resistance in tomato

    NARCIS (Netherlands)

    Aarts, J.M.M.J.G.

    1993-01-01

    Root knot nematode resistance in tomato is a genetic trait which is determined by a single dominant gene ( <em>Mi> ) on chromosome 6 of tomato. Information about the mRNA or protein product is completely lacking, which precludes the cloning of <em>Mi> by

  1. Comparing root architectural models

    Science.gov (United States)

    Schnepf, Andrea; Javaux, Mathieu; Vanderborght, Jan

    2017-04-01

    Plant roots play an important role in several soil processes (Gregory 2006). Root architecture development determines the sites in soil where roots provide input of carbon and energy and take up water and solutes. However, root architecture is difficult to determine experimentally when grown in opaque soil. Thus, root architectural models have been widely used and been further developed into functional-structural models that are able to simulate the fate of water and solutes in the soil-root system (Dunbabin et al. 2013). Still, a systematic comparison of the different root architectural models is missing. In this work, we focus on discrete root architecture models where roots are described by connected line segments. These models differ (a) in their model concepts, such as the description of distance between branches based on a prescribed distance (inter-nodal distance) or based on a prescribed time interval. Furthermore, these models differ (b) in the implementation of the same concept, such as the time step size, the spatial discretization along the root axes or the way stochasticity of parameters such as root growth direction, growth rate, branch spacing, branching angles are treated. Based on the example of two such different root models, the root growth module of R-SWMS and RootBox, we show the impact of these differences on simulated root architecture and aggregated information computed from this detailed simulation results, taking into account the stochastic nature of those models. References Dunbabin, V.M., Postma, J.A., Schnepf, A., Pagès, L., Javaux, M., Wu, L., Leitner, D., Chen, Y.L., Rengel, Z., Diggle, A.J. Modelling root-soil interactions using three-dimensional models of root growth, architecture and function (2013) Plant and Soil, 372 (1-2), pp. 93 - 124. Gregory (2006) Roots, rhizosphere and soil: the route to a better understanding of soil science? European Journal of Soil Science 57: 2-12.

  2. Interaction between root-knot nematodes and Solanum spp. : variation in pathogenicity, cytology, proteins and DNA = [De interactie tussen wortelknobbelnematoden en Solanum spp. : variatie in ziekteverwekkend vermogen, cytologie, eiwitten en DNA

    NARCIS (Netherlands)

    Beek, van der J.G.

    1997-01-01


    This thesis describes genetic variation in the root-knot nematodes <em>Meloidogyne hapla, M. chitwoodiem> and <em>M. fallax,em> particularly with respect to their pathogenicity on <em>Solanum> spp. Significant differences in virulence and

  3. Estudo comparativo da reabsorção radicular apical em pacientes bruxônomos e pacientes sem sinais clínicos de desgaste dentário Comparative study of apical root resorption in patients whith bruxism and without clinical signs of dental wearing

    Directory of Open Access Journals (Sweden)

    Rebeca Odebrech

    2004-05-01

    Full Text Available A reabsorção radicular, assim como o bruxismo, são temas amplamente abordados na literatura, contudo, a associação dos mesmos ainda permanece pouco documentada. Desse modo, esta pesquisa objetiva comparar pacientes portadores de bruxismo e pacientes sem sinais clínicos de desgaste dentário em relação à quantidade de reabsorção radicular apical nos incisivos superiores. Para tanto foram avaliados clínica e radiograficamente 64 pacientes, perfazendo um total de 256 incisivos acompanhados. Os resultados demonstram que há uma correlação positiva entre bruxismo e reabsorção radicular.Root resorption and bruxism are largely study in literature. However their association still remains few documented. The aim of this study is compare the quantity of apical root resorption in upper permanent incisors in patients with bruxism and without clinical signs of dental wearing. 64 patients (256 incisors were evaluated clinical and radiografically. The results shows the correlation between bruxism and root resorption.

  4. Plant Functional Traits Associated with Mycorrhizal Root Foraging in Arbuscular Mycorrhizal and Ectomycorrhizal Trees

    Science.gov (United States)

    Eissenstat, D. M.; Chen, W.; Cheng, L.; Liu, B.; Koide, R. T.; Guo, D.

    2016-12-01

    Root foraging for nutrient "hot spots" is a key strategy by which some plants maximize nutrient gain from their carbon investment in root and mycorrhizal hyphae. Foraging strategies may depend on costs of root construction, with thick roots generally costing more per unit length than thin roots. Investment in mycorrhizal hyphae, which are considerably thinner than roots, may represent an alternative strategy for cost-effective nutrient foraging, especially for thick-root species. Type of mycorrhiza may matter, as ectomycorrhizal (EM) fungi are more associated with longer hyphae and ability to mineralize organic matter than arbuscular mycorrhizal (AM) fungi. Among AM trees in both subtropical forests in SE China and in temperate forests in central Pennsylvania, USA, we found that tree species with thin roots proliferated their roots in soil patches enriched with mineral nutrients to a greater extent than species with thick roots. In addition, thick-root species were consistently colonized more heavily with mycorrhizal fungi than thin root species, although nutrient addition tended to diminish colonization. In a common garden in central Pennsylvania of both AM and EM tree species, we found that nutrient patches enriched with organic materials resulted in greater root and mycorrhizal fungal proliferation compared to those enriched with inorganic nutrients and that thick-root species proliferated more with their mycorrhizal fungi whereas thin-root species proliferated more with their roots. We further examined with many more species, patterns of root and mycorrhizal fungal proliferation in organic-nutrient-enriched patches. Foraging precision, or the extent that roots or mycorrhizal hyphae grew in the enriched patch relative to the unenriched patch, was related to both root thickness and type of mycorrhiza. In both AM and EM trees, thick-root species were not selective foragers of either their roots or hyphae. In thin-root species, there was strong selectivity in

  5. Root planing with Er:YAG laser X Gracey curette, a study in vitro in scanning electron microscopic study; Avaliacao em microscopia eletronica de varredura da superficie radicular, raspada e alisada com Er: YAG laser

    Energy Technology Data Exchange (ETDEWEB)

    Mello, Fabiano Augusto Sfier de

    2000-07-01

    The Er:YAG laser has been studied as a periodontal therapies, used to remove subgingival calculus and its bactericidal effects. The proposal of this study is to evaluate the effectiveness of the Er:YAG laser in root planing in comparison to the traditional method, not surgical. Six recently extracted due to the disease periodontal, were cut longitudinally, tends like this two half of the root. These half were separate in four groups. The first group is the natural tooth (group controls negative), the second group (group controls) was accomplished to root planing with Gracey curette. And in the third to Er:YAG laser with a contact tip, using a 45 deg angle in relation to the root; in the fourth group was scraped and planned with Er:YAG laser and complemented root planing with Gracey curette. The used energy was of 100 mJ (out put) and the frequency of 10 Hz accomplished with irrigation. The obtained results were similar in the groups 2 and 3 in comparison to the amount of smear-layer. In group 4 however, better result was obtained, because the image (SEM) was much more regular and with less amount of smear-layer. The conclusion of the work is that with the association of the laser technique and Gracey curette the results are superior to the conventional treatment. (author)

  6. Atividade de enzimas fermentativas em raízes e nódulos de soja sob condições de hipóxia e pós-hipóxia Fermentative enzymes activity in soybean roots and nodules under hypoxia and post-hypoxia conditions

    Directory of Open Access Journals (Sweden)

    Junior Borella

    2013-06-01

    Full Text Available O objetivo deste trabalho foi avaliar a atividade de enzimas fermentativas em raízes e nódulos de soja (Glycine max sob condições de hipóxia e pós-hipóxia. Os genótipos Fundacep 53 RR e BRS Macota foram cultivados em vasos de 3L contendo vermiculita como substrato em associação com Bradyrhizobium elkanii em casa de vegetação. No estádio R2, o sistema radicular das plantas foi submetido à hipóxia pelo encaixe de um segundo vaso sem perfurações e inundado com solução nutritiva diluída a 1/3 da concentração original, por 8 dias. Posteriormente, foram retirados os vasos sem perfurações para avaliação da recuperação. O material vegetal foi coletado no 2º, 4º, 6º e 8º dias após a inundação ou recuperação, juntamente com os controles para a dosagem da atividade das enzimas álcool desidrogenase (ADH, piruvato descarboxilase (PDC e lactato desidrogenase (LDH em raízes e nódulos. Os dados foram submetidos à ANOVA e comparados pelo teste de Tukey a 5% de probabilidade. As atividades da ADH, PDC e LDH foram fortemente estimuladas com a inundação, especialmente em nódulos, e diminuíram com a recuperação, em ambos os genótipos. Fundacep 53 RR respondeu de forma mais efetiva aos efeitos dos tratamentos.This study aimed to evaluate the activity of fermentative enzymes in soybean (Glycine max roots and nodules under hypoxia and post-hypoxia conditions. The soybean genotypes Fundacep 53 RR and BRS Macota associated with Bradyrhizobium elkanii were grown in 3L pots containing vermiculite as substrate under greenhouse conditions. Root system of plants at the R2 stage was subjected to hypoxia by blocking drainage of the pot, by fitting a second one without holes and flooded with nutrient solution diluted one-third of the original. The flooding of the plants was carried out for eight days and after, the pot without drilling was removed for recovery assessment. Non-waterlogged plants were kept as control. Activities of

  7. Dentin penetrability evaluation of three different dyes in root-end cavities filled with mineral trioxide aggregate (MTA Avaliação da penetração dentinária de três diferentes corantes em retrocavidades obturadas com agregado trióxido mineral (MTA

    Directory of Open Access Journals (Sweden)

    Beatriz Farias Vogt

    2006-04-01

    Full Text Available The purpose of this study was to evaluate the penetration of three dyes in MTA root-end fillings. In 30 single-rooted teeth, cavities for retrofilling were prepared with an ultrasound appliance and filled with MTA. The specimens were randomly assigned to three groups (n = 10 and immersed in the following solutions: 2% methylene blue (MET, 50% silver nitrate (NIT and 0.2% rhodamine B (ROD. Two transversal slices (1 mm of the retrofilling region were obtained and evaluated using the Image Tool 3.0 software to obtain a quantitative evaluation (in mm² of the dye penetration around the retrofillings. Data were submitted to statistical analysis using Student’s t-test. The lowest degree of dye penetration was observed for the NIT group, in both slices (p A proposta deste trabalho foi avaliar a penetração de três corantes em retrocavidades obturadas com MTA. As retrocavidades foram confeccionadas com aparelho de ultra-som em 30 dentes unirradiculares e obturadas com MTA. Os espécimes foram divididos aleatoriamente em três grupos (n = 10 e imersos nas seguintes soluções corantes: azul de metileno a 2% (MET, nitrato de prata a 50% (NIT e rodamina B a 0,2% (ROD. Duas fatias transversais (1 mm da região retrobturada foram obtidas e avaliadas através do software Image Tool 3.0, objetivando quantificar a área (em mm² de penetração do corante ao redor das retrobturações. Os dados foram submetidos à análise estatística, utilizando o teste t de Student. A menor área de penetração foi observada no grupo NIT, nas duas fatias (p < 0.05. A penetração de corante foi significativamente maior no grupo ROD quando comparado ao grupo NIT, nas duas fatias (p < 0.05, e ao grupo MET, somente na fatia 1 (p < 0.05. Dentro das limitações desta pesquisa, concluiu-se que a escolha da solução corante pode influenciar a avaliação da penetração em estudos sobre retrobturações e que o grupo NIT teve a menor capacidade de penetração na dentina

  8. Relationships between root diameter, root length and root branching along lateral roots in adult, field-grown maize

    OpenAIRE

    Wu, Qian; Pag?s, Lo?c; Wu, Jie

    2016-01-01

    Background and Aims Root diameter, especially apical diameter, plays an important role in root development and function. The variation in diameter between roots, and along roots, affects root structure and thus the root system?s overall foraging performance. However, the effect of diameter variation on root elongation, branching and topological connections has not been examined systematically in a population of high-order roots, nor along the roots, especially for mature plants grown in the f...

  9. Tooth Eruption without Roots

    OpenAIRE

    Wang, X.-P.

    2013-01-01

    Root development and tooth eruption are very important topics in dentistry. However, they remain among the less-studied and -understood subjects. Root development accompanies rapid tooth eruption, but roots are required for the movement of teeth into the oral cavity. It has been shown that the dental follicle and bone remodeling are essential for tooth eruption. So far, only limited genes have been associated with root formation and tooth eruption. This may be due to the diffic...

  10. Root production method system

    Science.gov (United States)

    Wayne Lovelace

    2002-01-01

    The RPM system (Root Production Method) is a multistep production system of container tree production that places primary emphasis on the root system because the root system ultimately determines the tree's survival and performance in its outplanted environment. This particular container production system has been developed to facilitate volume production, in a...

  11. Armillaria root rot

    Science.gov (United States)

    First described on grapevines in California in the 1880s, Armillaria root rot occurs in all major grape-growing regions of the state. The causal fungus, Armillaria mellea, infects woody grapevine roots and the base of the trunk (the root collar), resulting in a slow decline and eventual death of the...

  12. Root canal irrigation

    NARCIS (Netherlands)

    van der Sluis, L.; Boutsioukis, C.; Jiang, L.M.; Macedo, R.; Verhaagen, B.; Versluis, M.; Chávez de Paz, L.E.; Sedgley, C.M.; Kishen, A.

    2015-01-01

    The aims of root canal irrigation are the chemical dissolution or disruption and the mechanical detachment of pulp tissue, dentin debris and smear layer (instrumentation products), microorganisms (planktonic or biofilm), and their products from the root canal wall, their removal out of the root

  13. The Root Canal Biofilm

    NARCIS (Netherlands)

    van der Sluis, L.W.M.; Boutsioukis, C.; Jiang, L.M.; Macedo, R.; Verhaagen, B.; Versluis, Michel; Chávez de Paz, E.; Sedgley, C.M.; Kishen, A.

    2015-01-01

    The aims of root canal irrigation are the chemical dissolution or disruption and the mechanical detachment of pulp tissue, dentin debris and smear layer (instrumentation products), microorganisms (planktonic or biofilm), and their products from the root canal wall, their removal out of the root

  14. Avaliação nutricional e desempenho da silagem de raiz de mandioca contendo ou não soja integral em dietas para suínos = Nutritional evaluation and performance of cassava root silage with or without whole soybean in swine diets

    Directory of Open Access Journals (Sweden)

    Marcos Augusto Alves da Silva

    2010-04-01

    Full Text Available Dois experimentos foram conduzidos para determinar o valor nutritivo e o desempenho de suínos nas fases de crescimento e período total, alimentados com dietas formuladas com silagem de raiz de mandioca contendo ou não soja integral. No primeiro, foram utilizados 15 suínos, distribuídos em delineamento inteiramente casualizado em parcelas (animais subdivididas no tempo (subparcelas, totalizando cinco tratamentos e seis repetições. Os alimentos avaliados foram silagem de raiz de mandioca (MA, silagem de raiz de mandioca com inoculante (MI, silagem de raiz de mandioca + soja integral (MS e silagem de raiz de mandioca + soja integral com inoculante (MSI. As silagens apresentaram bons valores nutritivos e o uso de inoculante não foi efetivo para melhorar a digestibilidade dos nutrientes. No segundo, foram utilizados 36 suínos mestiços, distribuídos em um delineamento inteiramente casualizado, com restrição na casualização para duas classes de peso inicial, com três tratamentos, seisrepetições e dois animais por unidade experimental. Os tratamentos consistiram em raçãotestemunha à base de milho e farelo de soja (RT e outras duas com substituição total do milho por MA e MS. Para fase de crescimento, a conversão alimentar melhorou com o uso das silagens.Conclui-se que as silagens de mandioca, contendo ou não soja integral, apresentam bons valores nutritivos e podem substituir totalmente o milho na ração de suínos nas fases de crescimento eperíodo total.Two experiments were carried out to determine the nutritional value and performance of growing and total-period swine fed cassava root silage with or without whole soybean. In the first group, 15 crossbred swine were used, in a completely randomized design with parcels (animals subdivided in time (subparcels with five treatmentsand six replications. The study evaluated cassava root silage (CA, cassava root silage with inoculant (CI, cassava root silage + whole soybean (CS and

  15. Extração e análise eletroforética em gel de poliacrilamida (SDS-PAGE de proteínas totais de folhas e raízes de Piper tuberculatum Extraction and polyacrylamide gels electrophoresis analysis (SDS-PAGE of total protein from leaves and roots of Piper tuberculatum

    Directory of Open Access Journals (Sweden)

    Redinaldo dos Santos Silva

    2009-01-01

    Full Text Available O Estado do Pará é o principal produtor brasileiro de pimenta-do-reino (Piper nigrum Link, entretanto a sua produção tem sido bastante afetada pela doença conhecida como fusariose. O Fusarium solani f. sp. piperis é o agente causador desta doença que afeta o sistema radicular da planta, causando o apodrecimento das raízes e a queda das folhas levando à morte da planta. Algumas piperáceas nativas da região amazônica, entre elas a espécie Piper tuberculatum Jacq., têm se mostrado resistentes à infecção pelo F. solani f. sp. piperis, e desta forma têm sido utilizadas em estudos de interação planta-patógeno. Neste trabalho foram avaliadas cinco condições de extração de proteínas com o objetivo de selecionar tampões adequados para a extração de proteínas totais de folhas e raízes de P. tuberculatum. Os tampões utilizados para a extração de proteínas de raízes e folhas foram: tampão salino, tampão sacarose, tampão glicerol, tampão uréia e tampão fosfato de sódio. As análises quantitativas mostraram que os tampões sacarose, glicerol e uréia foram mais eficientes na extração de proteínas de folhas e raízes. Análises de SDS-PAGE mostraram padrões diferenciados de bandas em extratos protéicos de folhas e raízes obtidos com os diferentes tampões. Os resultados obtidos neste trabalho contribuem para a identificação de tampões de extração adequados para a obtenção de amostras de proteínas totais em estudos de interação P. tuberculatum - F. solani f. sp. piperis.The Pará State is the principal Brazilian producer of black pepper (Piper nigrum Link, however, the spice’s production has been damaged by the Fusarium disease. The Fusarium solani f. sp. piperis, causal agent of this disease which attacks the plant root system promoting the root rot, leaves fall and plant death. Piperaceae plants endemic of Amazon region and resistant to F. solani f. sp. piperis infection, such as Piper tuberculatum

  16. Variabilidade espacial do sistema radicular do tomateiro e implicações no manejo da irrigação em cultivo sem solo com substratos Spatial variability of tomato rooting system and implications on irrigation management in soiless cropping with substrates

    Directory of Open Access Journals (Sweden)

    Waldir A. Marouelli

    2005-03-01

    Full Text Available Avaliou-se a distribuição de raízes do tomateiro e a variabilidade horizontal do potencial matricial (psim e da condutividade elétrica da solução em substratos, de fibra de coco verde, casca de arroz carbonizada, maravalha carbonizada e comercial, acondicionados em sacos de plástico do tipo bisnaga. Cada planta foi irrigada por um único gotejador, posicionado a 7 cm da mesma. Maior concentração de raízes e menores valores de psim, sob condições de déficit hídrico temporário, ocorreram junto às plantas, nos substratos de fibra de coco e casca de arroz, e junto aos gotejadores, nos de maravalha e comercial. A baixa concentração de raízes entre um gotejador e a planta subseqüente, irrigada por outro emissor, ocorreu, provavelmente, pela alta condutividade elétrica da solução do substrato nessa região (até 8,3 dS m-1. As correlações entre concentração de raízes versus psim e concentração de raízes versus condutividade foram significativas para todos os substratos. A avaliação da variabilidade do psim nos substratos, em plantas submetidas a déficit hídrico, permitiu avaliar qualitativamente a distribuição do sistema radicular do tomateiro, de forma mais prática que pelo método direto. Para fins de manejo da água, recomenda-se instalar os sensores de umidade entre a planta e seu respectivo gotejador.The distribution of tomato rooting system and horizontal variability of matric potential (psim and electrical conductivity of the solution were evaluated on green coconut fiber, carbonized rice husk, carbonized coarse sawdust and commercial substrates packed in plastic bags. Each plant was irrigated by a single dripper, set up 7 cm apart. Higher root concentrations and lower psim, under temporary water deficit conditions, occurred close to the plant for coconut fibers and rice husk, and close to the dripper for coarse sawdust and commercial substrates. The lower root concentration between a dripper and the

  17. Evaluation of Antioxidant Activities of Aqueous Extracts and Fractionation of Different Parts of <em>Elsholtzia em>ciliata>

    Directory of Open Access Journals (Sweden)

    Yuangang Zu

    2012-05-01

    Full Text Available The aim of this study was to investigate the antioxidant and free-radical scavenging activity of extract and fractions from various parts of <em>Elsholtzia ciliataem>. The inflorescences, leaves, stems and roots of <em>E. ciliataem> were extracted separately and two phenolic component enrichment methods: ethyl acetate-water liquid-liquid extraction and macroporous resin adsorption-desorption, were adopted in this study. The antioxidant activities of water extracts and fractions of <em>E. ciliataem> were examined using different assay model systems <em>in vitroem>. The fraction root E (purified by HPD300 macroporous resin exhibited the highest total phenolics content (497.2 ± 24.9 mg GAE/g, accompanied with the highest antioxidant activity against various antioxidant systems <em>in vitroem> compared to other fractions. On the basis of the results obtained, <em>E. ciliataem> extracts can be used potentially as a ready accessible and valuable bioactive source of natural antioxidants.

  18. Parâmetros cinéticos e morfológicos da absorção de nutrientes em cultivares de milho com variabilidade genética contrastante Nutrient uptake kinetics and morphological traits of roots of maize cultivars with contrasting genetic variability

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    Delson Horn

    2006-02-01

    Full Text Available Genótipos de milho com variabilidade genética contrastante apresentam potenciais produtivos diferentes. Isso pode ser causado, pelo menos em parte, por diferenças morfológicas no sistema radicular e nos parâmetros cinéticos de absorção de nutrientes. Este trabalho objetivou quantificar esses parâmetros em três cultivares de milho. Foram comparados um híbrido simples (HS, um híbrido duplo (HD e uma variedade de polinização aberta (VPA. Determinaram-se os parâmetros de absorção (influxo máximo, Imax, constante de Michalies-Menten, Km, e concentração na solução onde a absorção cessa, Cmin para N, P, K, Ca e Mg, além de atributos morfológicos radiculares, em experimentos efetuados em câmara de crescimento, com solução nutritiva. A morfologia das raízes variou pouco entre os genótipos, provavelmente por causa do cultivo das plantas em meio líquido. As diferenças entre genótipos quanto aos parâmetros cinéticos de absorção dependeram do nutriente. O Imax diferiu entre os cultivares para P; o Km, para N e P, e o Cmin, para N e K. A VPA, por apresentar maior variabilidade genética, deveria apresentar menores valores para Km e Cmin do que os híbridos. Contudo, isso só aconteceu para P em relação ao Km. O HS, por apresentar maior potencial produtivo, deveria expressar os maiores valores para Imax, mas isso não ocorreu com nenhum dos macronutrientes avaliados. Portanto, a absorção de nutrientes não parece ser um fator determinante nas diferenças de rendimento de grãos entre genótipos de milho com bases genéticas contrastantes.Maize genotypes with contrasting genetic variability have different yield potential. This distinct yield potential may be partially caused by differences in the root system morphology or in the nutrient uptake kinetics parameters. This study aimed to assess such morphological and physiological plant attributes of three maize genotypes with different genetic bases. A single-cross hybrid

  19. Enraizamento de estacas herbáceas do abacateiro ‘Fuerte’ com diferentes períodos de imersão em solução de ácido indolbutírico = Rooting of herbaceous cuttings of ‘Fuerte’ avocado in solution of indol-butiric acid with different times of immersion

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    Ubirajara Ribeiro Mindêllo Neto

    2007-07-01

    Full Text Available Este trabalho foi realizado na Embrapa, Canoinhas, Estado de Santa Catarina, com o objetivo de verificar o efeito de diferentes tempos de imersão em solução de ácido indolbutírico (AIB no enraizamento de estacas herbáceas do abacateiro ‘Fuerte’. As estacas foram coletadas em 25/5/04 de plantas com um ano de idade e preparadas com diâmetroentre 4 a 6 mm e 10 cm de comprimento, permanecendo no terço superior duas folhas cortadas ao meio. Os tratamentos foram os seguintes: 5 e 30 segundos; 5 minutos; 1, 4, 8 e 12 horas em solução de 1000 mg L-1 de AIB. O delineamento experimental foi inteiramente casualizado, com sete tratamentos, quatro repetições e doze estacas por parcela. Após aaplicação do AIB, as estacas foram colocadas em bandejas de poliestireno expandido com 72 células, contendo turfa como substrato e levadas para enraizar em casa-de-vegetação com nebulização intermitente. Após 185 dias, foi realizada a avaliação do experimento. Os períodos de imersão rápida, por 5 e 30 segundos na solução de 1000 mg L-1 de AIB apresentaram-se mais favoráveis ao enraizamento das estacas, sendo superiores estatisticamente aos demais tempos de imersão. Os tempos de imersão superiores a uma hora apresentaram efeito fitotóxico. Recomenda-se para a estaquia herbácea de plantas juvenis do abacateiro ‘Fuerte’ a imersão por 30 segundos em solução de 1000 mg L-1 de AIB.This study was carried out at Embrapa, Canoinhas, state of Santa Catarina, Brazil, aiming to verify the effect of different times of immersion in solution of indol-butiric acid (IBA in the rooting of herbaceous cuttings of ‘Fuerte’ avocado. The cuttings were collected on May 25th, 2004, from one-yearold plants and prepared with a 4-6mm diameter and 10cm long, with 2 remaining leaves cut in the middle, in the upper third part. The treatments were the following: 5 and 30seconds; 5 minutes; 1, 4, 8 and 12 hours in solution of 1000 mg L-1 of IBA. The

  20. Prevalência de cárie radicular e condição periodontal em uma população idosa institucionalizada de Piracicaba - SP Prevalence of root caries and periodontal conditions in an elderly institutionalized population from Piracicaba - SP

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    Marcelo de Castro MENEGHIM

    2002-03-01

    Full Text Available A evolução dos procedimentos preventivos e curativos em Odontologia, verificados nas duas últimas décadas, levou a uma maior longevidade dos elementos dentários, acarretando com isso, uma maior demanda por tratamento periodontal e, em especial, cárie radicular no paciente idoso. O presente estudo teve como objetivo verificar as condições periodontais e lesões radiculares, de uma amostra de população institucionalizada do município de Piracicaba - SP. Nesse estudo, foram examinados 151 idosos com idade entre 54 e 93 anos. Os sextantes foram examinados e classificados do ponto de vista de saúde periodontal, necessidade de tratamento periodontal, presença de retração gengival e lesões radiculares, utilizando-se como critérios os indicadores da OMS (1999. Comparando-se duas faixas etárias: de pacientes com idade entre 50 e 75 anos e pacientes com idade acima de 75 anos, constatou-se que há um aumento na prevalência de lesões de cárie e outras afecções radiculares, como erosão e abrasão, indicando aumento na susceptibilidade a tais lesões com o passar da idade.Preventive and invasive dental procedures have improved in the last two decades, leading to a greater longevity of teeth. As a result, teeth are more susceptible to periodontal diseases and, consequently, to root caries. The purpose of this study was to verify the prevalence of both periodontal diseases and root caries in an institutionalized population from Piracicaba - SP, Brazil. In this study, 151 subjects, aging 54 to 93 years, were examined. The sample was divided into two groups: a group of subjects aging 50 to 75 years and a group of subjects over 75 years old. The sextants were examined and classified as to periodontal health, periodontal treatment needs, prevalence of gingival recession and root lesions. In conclusion, a high prevalence of root caries was verified. That indicates an increase in the susceptibility to these lesions, which is associated with

  1. Estímulo no crescimento e na hidrólise de ATP em raízes de alface tratadas com humatos de vermicomposto: I - efeito da concentração Improving lettuce seedling root growth and ATP hydrolysis with humates from vermicompost: I - effect of vermicompost concentration

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    Maria Rita Cardoso Rodda

    2006-08-01

    Full Text Available O vermicomposto contém uma concentração elevada de substâncias húmicas e já é bem conhecido o efeito do seu uso sobre as propriedades do solo. No entanto, a ação direta das substâncias húmicas sobre o metabolismo das plantas é menos conhecida. O objetivo deste trabalho foi avaliar o uso de humatos extraídos de vermicomposto de esterco de curral com KOH 0,1 mol L-1 sobre o desenvolvimento e metabolismo de ATP em plântulas de alface. Após a germinação, plântulas de alface foram tratadas com os humatos em concentrações que variaram de 0 a 100 mg L-1 de C, durante quinze dias. Foram avaliados o crescimento da raiz e a atividade das bombas de H+ isoladas da fração microssomal do sistema radicular. Foi observado aumento na matéria fresca e seca do sistema radicular, bem como no número de sítios de mitose, raízes emergidas do eixo principal, na área e no comprimento radiculares, com o uso do humato na concentração de 25 mg L-1 de C. Também foi observado, nessa concentração, aumento significativo na hidrólise de ATP pelas bombas de H+, responsáveis pela geração de energia necessária à absorção de íons e pelo crescimento celular.Vermicomposts present a high content of humic substances and their effects on soil properties are well-documented. However, direct effects of humic substances on plant metabolism are less known. The aim of this work was to evaluate the use of humates isolated from cattle manure vermicompost with 0.1 mol L-1 KOH on root growth and ATP metabolism of lettuce seedlings. After germination, lettuce seedlings were treated with different humate concentrations (0 to 100 mg L-1 of C for 15 days. The root growth and the H+-ATPase activity of root cell microsomes isolated by differential centrifugation were evaluated. The humate treatment, at the concentration of 25 mg L-1 of C, increased fresh and dry root mass as well as root area and total length. Also there was a large increase in the number of

  2. Morphologic analysis, by means of scanning electron microscopy, of the effect of Er: YAG laser on root surfaces submitted to scaling and root planing Análise morfológica, através de microscopia eletrônica de varredura, da ação do laser de Er: YAG em superfícies radiculares submetidas à raspagem e aplainamento radicular

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    Letícia Helena Theodoro

    2002-12-01

    Full Text Available The purpose of this study was to morphologically evaluate, by means of scanning electron microscopy, the effects of Er:YAG laser on the treatment of root surfaces submitted to scaling and root planing with conventional periodontal instruments. Eighteen root surfaces (n = 18, which had been previously scaled and planed, were assigned to 3 groups (n = 6. The control Group (G1 received no further treatment; Group 2 (G2 was irradiated with Er:YAG laser (2.94 mum, with 47 mJ/10 Hz, in a focused mode with air/water spray during 15 s and with 0.57 J/cm² of fluency per pulse; Group 3 (G 3 was irradiated with Er:YAG laser (2.94 mum, with 83 mJ/10 Hz, in a focused mode with air/water spray during 15 s and with 1.03 J/cm² of fluency per pulse. We concluded that the parameters adopted for Group 3 removed the smear layer from the root surface, exposing the dentinal tubules. Although no fissures, cracks or carbonized areas were observed, an irregular surface was produced by Er:YAG laser irradiation. Thus, the biocompatibility of the irradiated root surface, within the periodontal healing process, must be assessed.O objetivo do presente estudo foi analisar morfologicamente, através de microscopia eletrônica de varredura, os efeitos do laser de Er:YAG no tratamento de superfícies radiculares submetidas à raspagem e aplainamento radicular com instrumentos manuais. Foram utilizados 18 espécimes (n = 18 de superfícies radiculares que após ser submetidos à raspagem e aplainamento radicular foram divididos em 3 grupos (n = 6. O grupo controle (G1 não sofreu nenhum tratamento; Grupo 2 (G2 foi irradiado com laser de Er:YAG (2,94 mim 47 mJ/10 Hz, focalizado,com refrigeração à água durante 15 s e fluência de pulso de 0,57 J/cm²; Grupo 3 (G3 foi irradiado com laser de Er:YAG (2,94 mim, 83 mJ/10 Hz, focalizado, com refrigeração à agua durante 15 s e fluência de pulso de 1,03 J/cm². Através da análise dos resultados podemos concluir que o laser de

  3. Identification and Determination of <em>Aconitum> Alkaloids in <em>Aconitum> Herbs and <em>Xiaohuoluo Pillem> Using UPLC-ESI-MS

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    Li Yang

    2012-08-01

    Full Text Available A rapid, specific, and sensitive ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS method to examine the chemical differences between <em>Aconitum> herbs and processed products has been developed and validated. Combined with chemometrics analysis of principal component analysis (PCA and orthogonal projection to latent structural discriminate analysis, diester-diterpenoid and monoester-type alkaloids, especially the five alkaloids which contributed to the chemical distinction between <em>Aconitum> herbs and processed products, namely mesaconitine (MA, aconitine (AC, hypaconitine (HA, benzoylmesaconitine (BMA, and benzoylhypaconitine (BHA, were picked out. Further, the five alkaloids and benzoylaconitine (BAC have been simultaneously determined in the <em>Xiaohuoluo pillem>. Chromatographic separations were achieved on a C18 column and peaks were detected by mass spectrometry in positive ion mode and selected ion recording (SIR mode. In quantitative analysis, the six alkaloids showed good regression, (<em>r> > 0.9984, within the test ranges. The lower limit quantifications (LLOQs for MA, AC, HA, BMA, BAC, and BHA were 1.41, 1.20, 1.92, 4.28, 1.99 and 2.02 ng·mL−1, respectively. Recoveries ranged from 99.7% to 101.7%. The validated method was applied successfully in the analysis of the six alkaloids from different samples, in which significant variations were revealed. Results indicated that the developed assay can be used as an appropriate quality control assay for <em>Xiaohuoluo pillem> and other herbal preparations containing <em>Aconitum> roots.

  4. Conversão in vitro de raízes e folhas de Miltonia flavescens Lindl. em protocormos e regeneração de plantas In vitro conversion of Miltonia flavescens Lindl. roots and leaf tip cells in protocorm like bodies and plant regeneration

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    Suzana Stefanello

    2009-02-01

    Full Text Available Objetivou-se, no presente trabalho, avaliar o efeito do 2,4-D e do BAP na formação de protocormos a partir de regiões meristemáticas da raiz e segmentos foliares de Miltonia flavescens Lindl., bem como testar o efeito de concentrações de sacarose e níveis de pH no crescimento in vitro dos protocormos. Ápices radiculares e segmentos de folhas de plantas germinadas in vitro foram inoculados em meio de cultura MS/2 suplementado com 2,4-D e BAP onde permaneceram por 210 dias no escuro, sendo transferidos para a luz e inoculados em meio de cultura isento de reguladores, avaliando-se a porcentagem de explantes com protocormos e o número médio de protocormos regenerados por explante. A associação de 3 mg L-1 de 2,4-D e 1 ou 3 mg L-1 de BAP proporcionou os melhores resultados quanto à formação de protocormos. Esses foram cultivados em meio de cultura MS/2 onde testou-se o efeito da sacarose (0, 15, 30, 45 e 60 g L-1 e do pH (5.0, 5.8 e 6.6 sobre o seu crescimento. As concentrações de 15 e 30 g L-1 de sacarose independentes do pH, mostraram-se mais eficientes para o cultivo in vitro da espécie proporcionando a formação de um maior número e comprimento de raízes, altura de parte aérea e peso da matéria fresca. O pH só apresentou-se significativo quando em combinação com 15 g L-1 de sacarose para as variáveis comprimento da maior raiz e peso da matéria fresca.The aim of the present work was to evaluate the effect of 2,4-D and BAP on the protocorm formation from meristematic areas of the root and leaf segments of Miltonia flavescens Lindl., as well as to test the effect of sucrose concentrations and pH levels on the in vitro growth of protocorms. Root apexes and leaf segments of in vitro-germinated plants were cultured on MS/2 culture medium supplemented with 2,4-D and BAP and kept in the dark for 210 days. Explants were then transferred to light and cultured on culture medium lacking growth regulators. Percentage of explants

  5. Atuação tuação das enzimas oxidativas no escurecimento causado pela injúria por frio em raízes de batata-baroa = Role of oxidative enzymes on the darkening induced by chilling of arracacha roots

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    Luciana Nunes Menolli

    2008-01-01

    Full Text Available Neste trabalho, as raízes de batata-baroa (Arracacia xanthorrhiza Bancroft foram armazenas em câmaras frias, à temperatura de 5 e 10ºC, por 28 dias. A cada sete dias, as raízes eram retiradas da condição de frio, e realizada análise visual dos sintomas de injúriapor frio. Em seguida, as raízes foram maceradas para determinação das atividades da peroxidase, polifenoloxidase e da concentração dos compostos fenólicos solúveis. As temperaturas de 5 e 10ºC estimularam o escurecimento externo e interno das raízes durante o armazenamento, com maior intensidade para a temperatura de 5oC. Em ambas astemperaturas, houve elevação da atividade da polifenoloxidase, peroxidase e da concentração de compostos fenólicos, a partir da exposição dos tecidos ao frio. A atividade da polifenoloxidase e a concentração de compostos fenólicos solúveis aumentaram após o 14ºdia de exposição às duas temperaturas. A atividade da peroxidase aumentou até o 7º dia de armazenamento em ambas as temperaturas, mantendo-se praticamente constante, após este período, a 5 e a 10ºC, a maior atividade ocorreu no 14º dia de armazenamento. Estesresultados indicam atuação inicial da peroxidase como uma resposta inicial ao estresse causado pela colheita e o frio e posterior participação de polifenoloxidase no escurecimento enzimático e acúmulo de compostos fenólicos nos tecidos.In this experiment roots of arracacha (Arracacia xanthorrhiza Bancroft were stored at temperature of 5 and 10oC for 28 days. At every seven days, the roots were removed from the cold storage and visually analyzed for the presence of chilling symptoms. Afterwards, the roots were grinded for polyphenoloxidase, peroxidase activities and soluble phenolic compounds. Both temperature of 5 and 10oC induced external and internal darkening of the roots, with higher intensity at 5oC. Activities of polyphenoloxidase, peroxidase and content of phenolic compounds were enhanced at cold

  6. Effect of lincomycin treatment of root surface in dental replantation: a study in rats = Efeito da lincomicina como tratamento da superfície radicular no reimplante de dentes: estudo em ratos

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    Silva, Daniela Nascimento

    2009-01-01

    Full Text Available Objetivo: Estudos sobre antibióticos usados topicamente na superfície radicular de dentes avulsionados buscam avaliar suas propriedades anti-reabsortivas que favoreçam o reparo do ligamento periodontal e o restabelecimento da articulação dentoalveolar. Neste mesmo propósito, esta pesquisa avaliou o efeito da lincomicina na superfície radicular, antes do reimplante. Metodologia: Dez ratos foram distribuídos em dois grupos de 05 animais (I – controle e II – experimental. O incisivo superior direito dos animais foi extraído e estocado em leite por 30 min. Em seguida, no grupo I, os dentes foram irrigados com soro fisiológico e reimplantados; no grupo II, os incisivos foram imersos em lincomicina por 5 min, antes do reimplante. Os animais foram eutanasiados no 21º dia pós-operatório e a hemimaxila direita submetida a cortes histológicos, corados em HE, para avaliação microscópica. Resultados: No grupo I, o ligamento periodontal apresentou-se disperso, com infiltrado linfoplasmocitário moderado a intenso e a articulação dentoalveolar não foi restabelecida; no grupo II, notou-se completo reparo do ligamento, poucas células inflamatórias e pequenas áreas de reabsorção inflamatória e anquilose. Conclusões: o uso tópico da lincomicina no tratamento da superfície radicular promoveu o reparo do ligamento periodontal, reduziu o processo inflamatório, mas ainda foram observadas áreas de reabsorção inflamatória e anquilose

  7. Low-intensity laser coupled with photosensitizer to reduce bacteria in root canals compared to chemical control; Laser em baixa intensidade associado a fotosensibilizador para reducao bacteriana intracanal comparado ao controle quimico

    Energy Technology Data Exchange (ETDEWEB)

    Garcez Segundo, Aguinaldo Silva

    2002-07-01

    The photodynamic therapy is a process in which a dye is associate with an appropriate wavelength of light and this dye goes to an excited state. The excited reacts with oxygen to form the highly reactive compound singlet oxygen, and this compound can kill bacteria and tumor cells. The purpose of this study was to evaluate the bactericidal reduction in root canal contaminated with E. Faecalis. Thirty teeth with their root canals prepared were contaminated with E. faecalis. The teeth have received the chemical substance sodium hypochlorite for 30 minutes; ten teeth have received the azulene dye paste for 5 minutes and have been irradiated with a diode laser, output power 10 mW and {lambda}= 685 nm for 3 minutes. Ten teeth have not received treatment (control group). The bacterial reduction was significantly higher for laser group when compared to chemical and control groups. These results indicate photodynamic therapy as an effective method to kill bacteria. (author)

  8. Efficacy of three conditions of radiographic interpretation for assessment root canal length Eficácia de três condições de interpretação radiográfica em odontometria

    OpenAIRE

    Mitsuru Ogata; Leonor de Castro Monteiro Loffredo; Milton Carlos Kuga; Gulnara Scaf

    2005-01-01

    OBJECTIVE: To compare the efficacy of three conditions of image interpretation for radiographic root measurements and calculating the intra-observer reproducibility of the measurements. MATERIAL AND METHODS: Thirty intra-operative periapical radiographs of maxillary central and lateral incisors were measured, in mm, from the tip of the file to the radiographic apex, using a caliper. Three separate measurements were made of the 30 radiographs. The three measurements for each tooth were average...

  9. Influência do número de nós em estacas semilenhosas de oliveira (Olea europaea L. no enraizamento sob câmara de nebulização Influence the number of nodes on semi-woody cuttings of olive tree (Olea europaea L. on the rooting in misty room

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    Adelson Francisco de Oliveira

    2003-04-01

    Full Text Available Com o objetivo de avaliar o enraizamento de estacas semilenhosas de oliveira (Olea europaea L. com diferentes números de nós, foi conduzido no Centro de Investigación y Formación Agraria - CIFA "Alameda del Obispo" de Córdoba - Espanha, experimento sob condições de câmara de nebulização intermitente. As estacas foram tratadas com ácido indolbutítico (AIB na concentração de 3.000 mg.L-1, antes da instalação do ensaio, submergindo durante cinco segundos as suas bases, aproximadamente 2,5 centímetros, em solução contendo o produto. Utilizaram-se estacas medianas, que foram coletadas de plantas em desenvolvimento vegetativo contínuo. O delineamento experimental utilizado foi o de blocos casualizados em fatorial 2 x 5, sendo respectivamente duas variedades, Picual e Arbequina, e cinco tamanhos de estacas: dois nós com duas folhas, três nós com duas folhas, três nós com quatro folhas, quatro nós com quatro folhas e cinco nós com quatro folhas. Utilizaram-se quatro repetições com parcelas experimentais constituídas por trinta estacas plantadas em substrato de perlita, em mesas suspensas, com aquecimento controlado. Avaliaram-se aos 75 dias porcentagem de estacas enraizadas, mortas e número e comprimento médio de raízes. Observou-se que, entre estacas de quatro nós e cinco nós, com quatro folhas, não houve diferença nos parâmetros considerados; estacas com três nós, com duas e quatro folhas também não diferenciaram. Estacas com dois nós e duas folhas, de ambas as variedades, apresentaram maior mortalidade.With the objective of assessing the rooting efficiency of semi-woody cuttings of olive tree (Olea europaea L., with different nodes, an experiment under intermittent misty room conditions and with the use of IBA at the concentration of 3000 mg L-1 was run at the Centro de Investigación y Formación Agraria 'Alameda del Obispo' in Cordoba, Spain. The cuttings were treated before the establishment of the trial

  10. Genetic association among root morphology, root quality and root yield in ashwagandha (Withania somnifera)

    OpenAIRE

    Kumar Ramesh R.; Reddy Anjaneya Prasanna L.; Subbaiah Chinna J.; Kumar Niranjana A.; Prasad Nagendra H.N.; Bhukya Balakishan

    2011-01-01

    Ashwagandha (Withania somnifera) is a dryland medicinal crop and roots are used as valuable drug in traditional systems of medicine. Morphological variants (morphotypes) and the parental populations were evaluated for root - morphometric, quality and yield traits to study genetic association among them. Root morphometric traits (root length, root diameter, number of secondary roots/ plant) and crude fiber content exhibited strong association among them and ...

  11. Delayed tooth replantation after root surface treatment with sodium hypochlorite and sodium fluoride: histomorphometric analysis in rats Reimplante dentário tardio após o tratamento da superfície radicular com hipoclorito de sódio e fluoreto de sódio: análise histomorfométrica em ratos

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    André Dotto Sottovia

    2006-04-01

    Full Text Available In cases of delayed tooth replantation, non-vital periodontal ligament remnants have been removed with sodium hypochlorite in an attempt to control root resorption. Nevertheless, reports of its irritating potential in contact with the alveolar connective tissue have been described. Therefore, this study evaluated the healing process on delayed replantation of rat teeth, after periodontal ligament removal by different treatment modalities. Twenty-four rats, assigned to 3 groups (n=8, had their upper right incisor extracted and left on the workbench for desiccation during 60 min. Afterwards, the teeth in group I were immersed in saline for 2 min. In group II, root surfaces were scrubbed with gauze soaked in saline for 2 min; and in group III, scrubbing was done with gauze soaked in 1% sodium hypochlorite solution. Thereafter, root surfaces were etched with 37% phosphoric acid and immersed in 2% acidulate-phosphate sodium fluoride solution, at pH 5.5. Root canals were filled with a calcium hydroxide-based paste and the teeth were replanted. The animals were sacrificed 60 days postoperatively and the pieces containing the replanted teeth were processed and paraffin- embedded. Semi-serial transversally sections were obtained from the middle third of the root and stained with hematoxylin and eosin for histomorphometric analysis. Data were analyzed statistically using Kruskal-Wallis and Dunn's tests. The results showed that root structure and cementum extension were more affected by resorption in group III (pEm reimplante dentário tardio, o ligamento periodontal desvitalizado tem sido removido empregando-se o hipoclorito de sódio, buscando o controle da reabsorção radicular. Relatos de efeito irritante no tecido conjuntivo alveolar após o seu uso, têm sido descritos. Isso justificou a realização deste trabalho buscando minimizar esse inconveniente. Para isso 24 ratos, divididos em 3 grupos de 8 animais, tiveram o incisivo superior direito extra

  12. Tooth eruption without roots.

    Science.gov (United States)

    Wang, X-P

    2013-03-01

    Root development and tooth eruption are very important topics in dentistry. However, they remain among the less-studied and -understood subjects. Root development accompanies rapid tooth eruption, but roots are required for the movement of teeth into the oral cavity. It has been shown that the dental follicle and bone remodeling are essential for tooth eruption. So far, only limited genes have been associated with root formation and tooth eruption. This may be due to the difficulties in studying late stages of tooth development and tooth movement and the lack of good model systems. Transgenic mice with eruption problems and short or no roots can be used as a powerful model for further deciphering of the cellular, molecular, and genetic mechanisms underlying root formation and tooth eruption. Better understanding of these processes can provide hints on delivering more efficient dental therapies in the future.

  13. Cotton root and shoot growth as affected by application of mepiquat chloride to cotton seeds=Crescimento radicular e da copa do algodoeiro em função da aplicação de cloreto de mepiquat às sementes

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    Ciro Antonio Rosolem

    2012-01-01

    Full Text Available The effects of mepiquat chloride (MC application to cotton seeds on the initial growth of the plant roots and shoots were studied in a greenhouse experiment. Glass-front containers with 27 L of soil were used in the experiment. Cotton seeds of the cultivar FM 993 were sprayed with MC at five different doses of active ingredient (a.i.: 0, 3, 6, 9 and 12 g kg-1 seed. The shoot and root dry matter, leaf area, shoot:root ratio, leaf area: root growth ratio, and shoot length were evaluated 21 days after sowing. The root growth was evaluated every three days up to 18 days. The application of MC to cotton seeds decreased the shoot length but had no significant effect on the dry matter production, root length, shoot:root ratio or leaf area: root length ratio. Thus, the application of MC to cotton seeds is not expected to have a deleterious effect on plant water acquisition.Estudou-se o efeito do tratamento de sementes de algodão com cloreto de mepiquat sobre o crescimento inicial de raízes e parte aérea. O experimento, realizado em casa de vegetação, utilizou vasos de PVC adaptados com uma parede frontal de vidro e os tratamentos foram constituídos por cinco doses do cloreto de mepiquat (CM do ingrediente ativo (i.a.: 0, 3, 6, 9 e 12 g kg-1 de sementes, pulverizado sobre as sementes, e a cultivar FM 993. Massa de matéria seca da parte aérea (folhas, pecíolos e haste, massa de matéria seca da raiz, área foliar, relação parte aérea:raiz, relação área foliar:crescimento radicular, o comprimento da parte aérea foram avaliados aos 21 dias após a semeadura. Crescimento radicular foi avaliado a cada três dias até os 18 dias. O CM aplicado às sementes do algodão promove redução da altura da planta e da área foliar, sem, contudo, afetar produção de massa de matéria seca da parte aérea e raiz, relação parte aérea:raiz, relação área foliar:crescimento radicular e comprimento total de raízes do algodoeiro. Assim, no presente

  14. Conjoined lumbosacral nerve roots

    Directory of Open Access Journals (Sweden)

    Atila Yılmaz

    2012-03-01

    Full Text Available Lumbosacral nerve root anomalies are a rare group ofcongenital anatomical anomalies. Various types of anomaliesof the lumbosacral nerve roots have been documentedin the available international literature. Ttheseanomalies may consist of a bifid, conjoined structure, ofa transverse course or of a characteristic anastomizedappearance. Firstly described as an incidental findingduring autopsies or surgical procedures performed forlumbar disk herniations and often asymptomatic, lumbosacralnerve root anomalies have been more frequentlydescribed in the last years due to the advances made inradiological diagnosis.

  15. RUNTIME DICTIONARIES FOR ROOT

    CERN Document Server

    Wind, David Kofoed

    2013-01-01

    ROOT is the LHC physicists' common tool for data analysis; almost all data is stored using ROOT's I/O system. This system benefits from a custom description of types (a so-called dictionary) that is optimised for the I/O. Until now, the dictionary cannot be provided at run-time; it needs to be prepared in a separate prerequisite step. This project will move the generation of the dictionary to run-time, making use of ROOT 6's new just-in-time compiler. It allows a more dynamic and natural access to ROOT's I/O features especially for user code.

  16. Fine root productivity and turnover of ectomycorrhizal and arbuscular mycorrhizaltree species in a temperate broad-leaved mixed forest

    Directory of Open Access Journals (Sweden)

    Petra Kubisch

    2016-08-01

    Full Text Available Advancing our understanding of tree fine root dynamics is of high importance for tree physiology and forest biogeochemistry. In temperate broad-leaved forests, ectomycorrhizal (EM and arbuscular mycorrhizal (AM tree species often are coexisting. It is not known whether EM and AM trees differ systematically in fine root dynamics and belowground resource foraging strategies. We measured fine root productivity (FRP and fine root turnover (and its inverse, root longevity of three EM and three AM broad-leaved tree species in a natural cool-temperate mixed forest using ingrowth cores and combined the productivity data with data on root biomass per root orders. FRP and root turnover were related to root morphological traits and aboveground productivity.FRP differed up to twofold among the six coexisting species with larger species differences in lower horizons than in the topsoil. Root turnover varied up to fivefold among the species with lowest values in Acer pseudoplatanus and highest in its congener A. platanoides. Variation in root turnover was larger within the two groups than between EM and AM species. We conclude that the main determinant of fine root productivity and turnover in this mixed forest is species identity, while the influence of mycorrhiza type seems to be less important.

  17. Morphometrical analysis of cleaning capacity using nickel-titanium rotary instrumentation associated with irrigating solutions in mesio-distal flattened root canals Análise morfológica da capacidade de limpeza promovida pela instrumentação rotatória, associada à soluções irrigantes, com limas de níquel-titânio em canais radiculares com achatamento mesio-distal

    Directory of Open Access Journals (Sweden)

    Melissa Andréia Marchesan

    2003-03-01

    Full Text Available Variations in the internal anatomy of root canals may interfere with the success of root canal because tissue remnants may remain in the isthmus, re-entrances and ramifications of flattened root canals making instrumentation more difficult. Successful root canal treatment depends fundamentally on shaping, cleaning, disinfection and obturation. This study verified the quality of cleaning of ProFile .04 rotary technique associated with different irrigating solutions: 0.5% sodium hypochlorite, calcium hydroxide + Tergentol and 2% chlorhexidine in canals with mesio-distal flattening. Twelve human mandibular central incisors were randomly divided into 3 groups and instrumented up to ProFile 0.4 #35 file after cervical preparation, and processed histologically. After optical microscopic evaluation, statistical analysis showed that the percentage of cleaning of the three irrigating solutions was statistically different. Two-by-two comparisons classified the irrigating solutions in increasing order of cleaning: calcium hydroxide + Tergentol As variações da anatomia interna de cada canal radicular podem interferir no sucesso da terapêutica endodôntica devido ao fato de que em canais radiculares achatados, pode persistir remanescentes teciduais em istmos, reentrâncias e ramificações dificultando a execução das técnicas de instrumentação. O sucesso do tratamento dos canais radiculares depende fundamentalmente do preparo da forma, limpeza, desinfecção e obturação. Poucos trabalhos avaliaram a limpeza dos canais radiculares após a instrumentação rotatória. O presente trabalho verificou a qualidade de limpeza dos canais radiculares, por meio da microscopia óptica, promovida pela técnica de instrumentação rotatória associada ao hipoclorito de sódio 0,5%, HCT20 e clorexidina, em canais achatados no sentido mésio-distal. Doze incisivos centrais inferiores humanos foram divididos aleatoriamente em três grupos para que fossem

  18. UTILIZAÇÃO DA MANDIOCA (Manihot utilissima, Pohl CRUA NUM ESTUDO COMPARATIVO DE TRÊS DIETAS PARA SUÍNOS EM CRESCIMENTO-ENGORDA USE OF RAW CASSAVA ROOTS (Manihot utilissima POHL IN A COMPARATIVE STUDY OF THREE SWINE GROWING AND FATTENING DIETS

    Directory of Open Access Journals (Sweden)

    Francisco Eduardo Galvão

    2007-09-01

    Full Text Available

    O presente experimento foi conduzido na Escola Média de Agricultura de Florestal, Minas Gerais, com o fim de verificar a viabilidade do uso da mandioca (Manihot utilissima Pohl “in natura” em dietas de suínos em crescimento e engorda. Foram usados três tratamentos constituídos de mistura comercial para crescimento mais mandioca "in natura" (A, mistura comercial para engorda (B, fubá de milho (C recebendo todos verde à vontade. Em cada tratamento foram usados 10 animais mestiços com 133, 5 dias de idade e peso médio inicial de aproximadamente 26,1Kg. O experimento teve duração de 210 dias e os ganhos diários em peso foram de 0,451; 0,452 e 0,178 Kg, para os tratamentos A, B e C respectivamente. As análises de variância revelaram, que para ganhos em peso e espessura de toucinho, não houve diferenças estatisticamente significativas entre leitegadas e os tratamentos A e B, havendo porém (p < 0,01 entre tratamento C contra A e B. O tratamento contendo mandioca "in natura” revelou ser o mais econômico.

    An experiment was carried out at the Escola Média de Agricultura de Florestal, Minas Gerais, in order to verify the viability of cassava roots use (Manihot utilissima, Pohl ";in natura"; in growing and fattening swine diets. Three treatments were used, containing commercial mixture for fatteningswine (B, ground corn (C and all receiving para grass (Brachiaria mutica Stapf ";ad libitum";. In each treatment were used ten crossbred animals of 133,5 days and gains were 0,451; 0,452 and 0,178 kg for treatments A, B and C, respectively. The analysis of

  19. Efeito de três fertilizantes acidificantes sobre a concentração de alumínio e de manganês em folhas e raízes de cafeeiros Effect of three acidifying fertilizers on the concentration of aluminum and manganese on coffee leaves and roots

    Directory of Open Access Journals (Sweden)

    Ferdinando Roberto Pupo de Moraes

    1979-01-01

    Full Text Available Foi determinada a concentração de alumínio e de manganês em folhas de cafeeiros cultivados em vasos com três solos diferentes e com aplicação de três fertilizantes nitrogenados acidificantes (nitrato de amônio, uréia e sulfato de amônio e um não acidificante (salitre-do-chile, além de dois tratamentos extras com corretivos de acidez e um com enxofre. Nas raízes estes elementos foram também determinados para os tratamentos que receberam uréia, uréia mais enxofre e o controle sem nitrogênio. Nos três solos determinou-se o efeito dos tratamentos sobre o pH e a concentração de A1(3+ e Mn3+. A análise foliar revelou diferenças significativas entre tratamentos na concentração de alumínio e manganês das folhas e em todos os três solos estudados. Alguns sintomas específicos observados nas folhas e raízes estiveram associados à presença de níveis elevados de manganês nessas partes vegetais.There were determined concentrations of aluminum and manganese in leaves of coffee trees cultivated in pots with 3 different soils fertilized with acidifying nitrogen fertilizers (ammonium nitrate, urea and ammonium sulphate and non acidifying nitrogen fertilizer (chilean nitrate and 3 other treatments (urea plus calcium carbonate, urea plus lime and urea plus sulfur. Aluminum and manganese were determined in coffee roots of pots fertilized with urea, urea plus sulfur and a control without nitrogen. The relation of pH and concentration of Al3+ and Mn2+ are discussed for the soils utilized. Leaves analysis showed significant differences in aluminum and manganese concentration in the leaves among treatments and among the 3 soils types. Symptoms correlated with high levels of manganese in the leaves and roots of the coffee trees were observed.

  20. Contribuição do porta-enxerto, no teor de cafeína em grãos de café Contribution of the root-stock on the caffeine content of the coffee grains

    Directory of Open Access Journals (Sweden)

    M. Melo

    1976-01-01

    Full Text Available A contribuição do porta-enxerto no teor de cafeína dos grãos de café foi investigada nos cultivares mundo novo e laurina de Coffea arabica e no robusta de C. canephora, os quais diferem marcadamente no teor desse alcalóide. Os resultados obtidos indicam que o porta-enxerto, independentemente de seu genótipo, não exerce efeito mensurável sobre o teor de cafeína nos grãos. Variações individuais foram verificadas no teor de cafeína no robusta, devido à sua variabilidade genética, indicando a possibilidade de seleção para essa característica dentro desse material. Do ponto de vista agrícola o robusta poderia ser usado como porta-enxerto para as seleções de C. arabica, sem qualquer efeito no produto final.The contribution of the root-stock on the caffeine content of the coffee grains was studied in the Mundo Novo and Laurina cultivais of Coffea arabica and in the Rubosta cultivar of C. canephora which markedly differ in the alkaloid content. The results indicate that the root-stock, independently of its genotype, does not influence the caffeine content of the grains. Individual variation in caffeine content was found in the Robusta due to its genetic variability, pointing out to the possibility of selection for this characteristics in this material. It was also concluded that Robusta cultivars can be used as root-stock for Arabica selections without affecting the final product.

  1. Crescimento radicular e produção de ácidos orgânicos em cultivares de soja com diferentes tolerâncias ao alumínio Root growth and production of organic acids by soybean cultivars with different tolerance to aluminum

    Directory of Open Access Journals (Sweden)

    Orival Gastão Menosso

    2001-11-01

    Full Text Available A elucidação dos mecanismos que definem o comportamento diferencial entre genótipos de soja quanto à toxidez de Al facilita a utilização da variabilidade genética existente ou produzida. O objetivo deste trabalho foi avaliar a influência do Al no crescimento radicular, na modificação do pH da solução e no conteúdo de ácidos orgânicos em extratos de raízes de cultivares de soja tolerantes ao Al, FT-1 e FT-6 (Veneza e sensíveis, IAC-13 e Paraná. As plantas cresceram por nove dias em solução contendo 50 mg L-1 de Ca e 0,0 e 0,2 mg L-1 de Al, com pH inicial de 4,76. Houve maior crescimento de raízes nas cultivares tolerantes e não houve alterações do pH da solução relacionadas à tolerância das cultivares ao alumínio. A presença do Al reduziu o conteúdo dos ácidos cítrico, lático, succínico, oxálico e málico, em ambos os grupos de cultivares avaliados, porém com maior redução no grupo das cultivares sensíveis. O ácido cítrico foi encontrado em maior quantidade nas cultivares tolerantes. A capacidade das cultivares de soja FT-1 e FT-6 (Veneza de alterar o conteúdo de ácidos orgânicos não-voláteis, principalmente o ácido cítrico, que pode complexar o Al, indica que este mecanismo pode ser muito importante para a tolerância a esse elemento.The understanding of the mechanisms for the differential performance of soybean genotypes in relation to Al toxicity is important to explore the existing or produced genetic variability. The objective of this study was to determine the Al influence on root growth, solution pH modifications and organic acid content in root extracts of Al tolerant, FT-1 and FT-6 (Veneza and sensitive, IAC-13 and Paraná, soybean cultivars. Plants were grown during nine days in a solution containing 50 mg L-1 of calcium and 0.0 and 0.2 mg L-1 of aluminum. The solution pH at the beginning was 4.76. Root growth in Al solutions of the tolerant soybean cultivars was higher than that of the

  2. Hydroxyl and calcium ions diffusion from endodontic materials through roots of primary teeth - in vitro study Avaliação in vitro da difusão de íons Ca+2 e OH- de materiais endodônticos em dentes decíduos

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    Ana Cristina Gerent Petry Nunes

    2005-06-01

    Full Text Available The purpose of this research was to evaluate the diffusion of calcium (Ca+2 and hydroxyl (OH- ions from materials with a calcium hydroxide base - Ca(OH2 through the intact roots of deciduous teeth. This diffusion of ions is important for periapical healing. Forty-six deciduous teeth were selected and instrumented to their working length with #40 files. The teeth were washed during cleaning and shaping with a 1% sodium hypochlorite (NaOCl solution. The canals were dried with paper points. The teeth were divided into 4 groups based on the sealer type, with 10 specimens in each group. A fifth group of 6 teeth without sealer constituted the control group. The materials used as sealers were: Ca(OH2 paste associated to propylene glycol (CaPE thickened at the proportion of 2:1 w/v; UFSC (Federal University of Santa Catarina paste - a mixture of 0.3g of zinc oxide with 0.3g of Ca(OH2 with 0.2ml of olive oil 1:1 w/w; Vitapex® and Sealapex®. The coronal access was sealed with a glass ionomer after the root had been filled with each sealer. A one-third apical surface and foramen was hard-pressed with Araldite®. The teeth were stored individually in flasks containing saline solution at 37ºC and 100% humidity. The OH- and Ca+2 ions diffusion levels were determined using a pH meter and an atomic absorption spectrometer. Data were collected at 48 hours and at intervals of 7, 30, 45 and 60 days. Statistical analysis was performed using ANOVA to compare groups. In the pH evaluation, the CaPE group presented the largest OH- ions diffusion, which peaked at sixty days (p=0.0309, when compared to the other groups (pO objetivo desta pesquisa foi avaliar a difusão de íons Ca+2 e OH- de materiais endodônticos a base hidróxido de cálcio - Ca(OH2, através da raiz intacta de dentes decíduos. 46 dentes decíduos foram selecionados e instrumentados em seu comprimento de trabalho até a lima # 40, e irrigados durante o preparo com solução de hipoclorito de s

  3. The roots of nodulins.

    NARCIS (Netherlands)

    Nap, J.P.H.; Bisseling, T.

    1990-01-01

    Nodulin gene expression is an integral and highly specific part of the formation of nitrogenfixing nodules on the roots of leguminous plants. Dependent on the time of expression during root nodule development, nodulin genes can be divided into early and late nodulin genes. A brief overview of the

  4. Irrational Square Roots

    Science.gov (United States)

    Misiurewicz, Michal

    2013-01-01

    If students are presented the standard proof of irrationality of [square root]2, can they generalize it to a proof of the irrationality of "[square root]p", "p" a prime if, instead of considering divisibility by "p", they cling to the notions of even and odd used in the standard proof?

  5. Variação de carboidratos e ácido cianídrico em raízes de mandioca, após a poda da parte aérea Carbohydrates and hydrocyanic acid variation in cassava roots which aereal portions were totally pruned

    Directory of Open Access Journals (Sweden)

    José Osmar Lorenzi

    1978-01-01

    Full Text Available São relatados resultados do estudo da variação de matéria seca, HCN, amido, carboidratos solúveis totais e açúcares redutores em raízes de mandioca (Manihot esculenta, Crantz nos dias que sucederam à poda ou remoção total da parte aérea. Na amostragem foi utilizado o método convencional: raízes tomadas ao acaso e representativas da população. Diferentes tipos de raízes (da base e meio da maniva e da base das hastes coletadas 17 dias após a poda apresentaram teores de carboidratos bem diferentes, seja pela mobi-lização mais acentuada de reservas, seja pela sua constituição originalmente diversa, o que sugere pesquisas mais detalhadas sobre o método de amostragem das raízes para análise de laboratório. Verificou-se que, num período de quatorze dias após a poda de plantas com treze meses de idade e em época quente e chuvosa, as transformações mais profundas ocorreram no teor de amido, que decresceu de 79,06% para 62,52%, e nos carboidratos solúveis totais e açúcares redutores, que tiveram um incremento na matéria seca das raízes, respectiva-mente de 7,65% e 1,55% para 17,56% e 5,03%. 0 teor de carboidratos totais foi pouco afetado pela poda, Indicando perda pequena destinada a nova brotação ou a outros processos fisiológicos. O teor de HCN decresceu de 67 para 35 ppm.Data are presented on the variation of the level of dry matter, cyanide, starch, total soluble carbohydrates, and reducing sugars content in roots of 13 months old cassava (Manihot esculenta, Crantz at successive stages after the total removal of the aereal portions. During the 14 days following pruning starch percentage decreased from 79.06% to 62.52%; total soluble carbohydrates and reducing sugars showed a variation from 7.65% and 1.55% up to 17.56% and 5.03% respectively. Total carbohydrate presented small losses by pruning probably due to the new sprouting process. Root cyanid content decreased from 67 to 35 ppm. Its has also been observed

  6. Lead phytotoxicity on germination and early growth of lettuce as a function of root anatomy and cell cycle = Fitotoxicidade do chumbo na germinação e crescimento inicial de alface em função da anatomia radicular e ciclo celular

    Directory of Open Access Journals (Sweden)

    Marcio Paulo Pereira

    2013-04-01

    Full Text Available This work aimed to evaluate the phytotoxicity of five lead concentrations on germination, root anatomy and cell cycle of lettuce. Lettuce cypselae were placed in Petri dishes covered with filter paper and 2.5 mL of solution containing 0.0, 0.5, 1.0, 2.0 and 5.0 mM of Pb(NO32. Dishes were maintained in growth chamber B.O.D. type at 25ºC and photoperiod of 12 h, the following variables were evaluated: germination percentage, early growth, root anatomy, mitotic index and the rate of chromosomal abnormalities on root apical meristem. The seed germination and seedlings early growth were bothreduced proportionally to the increase in lead concentrations. The root anatomy showed decreases in apoplastic barriers andmodifications on the xylem related to the increase of hydraulic conductivity in the presence of lead. There were reductions inthe mitotic index and na increase in chromosomal abnormalities under the different lead concentrations. Therefore, the lettuceroot system were more affected by lead concentrations and the phytotoxicity symptoms are related to the reductions on mitoticindex on root apical meristem and anatomical modifications related to higher lead translocation to plant shoots.Objetivou-se com esse trabalho avaliar a fitotoxicidade de cinco concentrações de chumbo na germinação,anatomia radicular, e divisão celular de alface. Cipselas de alface foram colocadas em placas de Petri com papel de filtro e2,5 mL de solução contendo 0,0; 0,5; 1,0; 2,0 e 5,0 mM de Pb(NO32. As placas permaneceram em câmara do tipo B.O.D. a25°C com fotoperíodo de 12 h, sendo avaliadas as seguintes variáveis: germinação, crescimento inicial, anatomia radicular,índice mitótico e frequência de anormalidades cromossômicas no meristema apical radicular. O delineamento estatístico foiinteiramente casualizado com cinco tratamentos e quatro repetições. Os dados foram submetidos à análise de variância eregressão. A germinação das sementes e o

  7. SURVEY, IDENTIFICATION AND EVALUATION OF INSECT INJURIES IN ORNAMENTAL TREES IN THE URBAN AREA OF GOIÂNIA, GOIÁS STATE, BRASIL LEVANTAMENTO, IDENTIFICAÇÃO E AVALIAÇÃO DOS DANOS DE INSETOS EM ÁRVORES ORNAMENTAIS NA ÁREA URBANA DE GOIÂNIA (GO

    Directory of Open Access Journals (Sweden)

    Antônio Henrique Garcia

    2007-09-01

    Full Text Available

    From 1800 ornamental trees sampled in Goiânia urban area, <em>Paquira> <em>aquatica> represented 62,7 %, <em>Cesalpinia> <em>sp.> 15,3 %, <em>Bauhinia variegataem> 10 %, <em>Sapthodea> <em>campanulata> 6 %, <em>Terminalia> <em>catappa> 3,2 %, <em>Acacia> <em>sp.> 1,2%, <em>Ponciana> <em>sp.> 1,0 %, and other species 1 %. The most frequent insect species observed in P. aquatica were <em>Psylla> <em>floccosa> ( 92,3%, <em>Steirastoma> <em>breve> (76,7%, <em>Palindia> <em>detracta> (63,4%, <em>Phelypera> <em>shuppeli> (43,4% and <em>Euchroma> <em>gigantea> (23,7%. The most severe injuries to plants are caused by <em>S. breveem>, which desiccates trunk and stems, and <em>E. giganteaem>. Its larvae destroy the whole root system and cause the fall of the tree. <em>Orthezia> <em>praelonga> was observed in 84,6 % of the sampled <em>B. variegataem>; in 30 trees 20% died after three years and 40% at the of the fourth year; from 50 seedlings 36% were desiccated after one year and 58% 36 months later. <em>O. praelongaem> was observed in 94,6% of <em>S. campanulataem>; in 32 trees 8% were desiccated in one year, 18% in the second and the remaining at the end of the third year. <em>Icerya purchasiem> was observed in 84,7% of <em>Cesalpinia spem>.; from 48 trees 5% were desiccated after six, 16 % two years later and 30% at the end of the fourth year; from 50 seedlings of this species, under intense attack, 78% were desiccated in 15 days. Quesadas gigas e <em>Dorisiana sp.em> were observed in <em>Acacia sp.em> In <em>Ponciana sp.em> it was observed the presence of some species of <em>Scolytidae>, <em>Platipodidae> and <em>Rhinotermitidae>, and no injuries were observed in <em>Terminalia> <em>catappa>.

    KEY-WORDS: <em

  8. Enraizamento de estacas lenhosas de pereira tratadas com AIB e mantidas em ambiente de estufa tipo B.O.D. e de telado Rooting of hardwood cuttings of pear tree with IBA under B.O.D. chamber and greenhouse environment

    Directory of Open Access Journals (Sweden)

    Wilson Barbosa

    2007-01-01

    Full Text Available Pesquisou-se o enraizamento da pereira em ambiente controlado de estufa tipo B.O.D. e em telado simples. Utilizou-se a cultivar híbrida 'Limeira', destinada exclusivamente para fins culinários e para porta-enxerto. Estacas lenhosas sem folhas, medindo 25 cm de comprimento, foram tratadas com ácido indolbutírico (AIB nas concentrações de 0; 2.000; 4.000 e 6.000 mg.L-1 por 10 segundos. Como substrato, utilizou-se da mistura de vermiculita e areia grossa (2:1 v/v, sendo a mesma umedecida com meio contendo solução salina MS e sacarose 1%. As estacas permaneceram por 42 dias dentro de estufas tipo B.O.D. (temperatura de 25ºC, umidade relativa do ar de 90% e fotoperíodo de 8 horas e de telado com irrigação por microaspersão, sem controle ambiental. Em ambiente controlado de estufa, as estacas não-tratadas com AIB iniciaram intensa brotação das gemas e formação de calo após sete dias do plantio. Já em ambiente de telado, essas estacas demoraram 21 dias para o início de brotação das gemas, mostrando menor desenvolvimento de calo. Nas estacas tratadas com AIB, os calos surgiram nas regiões dos cortes após o terceiro dia de incubação na estufa B.O.D. As raízes desenvolveram-se a partir dos tecidos da base e dos calos, tornando-se mais nítidas a partir de 14 e 28 dias, respectivamente, para os ambientes de estufa e de telado. Após 42 dias, o melhor percentual de enraizamento (83% foi verificado no tratamento com AIB a 2.000 mg.L-1, em ambiente de estufa B.O.D. O emprego dessa estufa, com temperatura, luz e umidade relativa controladas, mostrou-se viável em relação ao telado, no processo de enraizamento das estacas lenhosas da pereira 'Limeira', podendo favorecer o sistema de propagação vegetativa da pereira e encurtar o período da formação de mudas.The rooting of cuttings pear under controlled environment of chamber (B.O.D. type and greenhouse was searched. Hardwood cuttings of 'Limeira' pear, without leaves, measuring

  9. Isolation and characterization of altered root growth behavior and ...

    African Journals Online (AJOL)

    Ezedom Theresa

    2013-10-02

    Oct 2, 2013 ... (EMS), for their root growth behavior and salinity tolerance under hydroponic conditions. Six independent mutant lines .... Nagina 22 mutants were screened under hydroponic conditions and putative ..... Nacl resistant rice plant lines from another culture: Distribution pattern of K+/Na+ in callus and plant cells ...

  10. Isolation and characterization of altered root growth behavior and ...

    African Journals Online (AJOL)

    In this study, we have screened about 1500 mutants (M2 generation) generated by treating an upland drought tolerant genotype Nagina 22 with Ethyl Methane Sulfonate (EMS), for their root growth behavior and salinity tolerance under hydroponic conditions. Six independent mutant lines possessing significantly shorter ...

  11. Produtividade agrícola de variedades de cana-de-açúcar e incidência de broca-comum e cigarrinha-da-raiz em canavial colhido sem queima Agricultural productivity of sugarcane varieties and incidence of moth borer and root froghopper in green sugarcane

    Directory of Open Access Journals (Sweden)

    Zigomar Menezes de Souza

    2008-01-01

    Full Text Available Com o uso de variedades adaptadas e o manejo do solo pós-colheita da soqueira de cana-de-açúcar em sistema de colheita mecanizada, poderá ser afetada a incidência de pragas e a produtividade do canavial. Assim, desenvolveu-se o presente trabalho com o objetivo de se avaliar a influência da palhada de dezoito variedades de cana-de-açúcar submetidas a diferentes sistemas de manejo de colheita mecânica, sem queima, na produtividade agrícola e na incidência de broca-comum e cigarrinha-das-raízes na cultura de cana-de-açúcar. Os tratamentos foram constituídos pela presença da palha sem trituração/sem cultivo (PST/SC; palha sem trituração/com cultivo com escarificador (PST/CC; palha triturada/com cultivo com escarificador (PT/CC. O rendimento de colmos na cana-soca é favorecido pelo manejo, com cultivo, da palhada da planta crua, colhida mecanicamente, sem alteração na incidência de broca-comum e da cigarrinha das raízes. A variedade SP87-365 de cana-de-açúcar é opção viável e rentável, em qualquer sistema de manejo da cana crua, com adaptação à colheita mecânica e às condições edafoclimáticas da região de Ribeirão Preto, SP. A incidência de broca-comum e da cigarrinha das raízes pode ser bastante reduzida com a utilização das variedades SP88-817 e SP76-112 de cana-de-açúcar, em qualquer sistema de manejo da palhada da cana crua colhida mecanicamente e minimizada na colheita mecânica da cana crua, seguida de trituração da palhada e cultivo com escarificador na entrelinha.The use of adapted varieties of sugarcane and the soil management after its harvest in mechanized systems may affect the incidence of pests and the productivity of the sugarcane plantation. The objective of this work was to evaluate the influence of plant residues of 18 sugarcane varieties submitted to different harvest management systems on the agricultural productivity and incidence of moth borer and root froghopper. The treatments

  12. Genetic association among root morphology, root quality and root yield in ashwagandha (Withania somnifera

    Directory of Open Access Journals (Sweden)

    Kumar Ramesh R.

    2011-01-01

    Full Text Available Ashwagandha (Withania somnifera is a dryland medicinal crop and roots are used as valuable drug in traditional systems of medicine. Morphological variants (morphotypes and the parental populations were evaluated for root - morphometric, quality and yield traits to study genetic association among them. Root morphometric traits (root length, root diameter, number of secondary roots/ plant and crude fiber content exhibited strong association among them and showed significant positive genotypic correlation with yield. Starch-fiber ratio (SFR, determinant of brittle root texture showed strong negative association with root yield. The total alkaloid content had positive genotypic correlation with root yield. So genetic upgradation should aim at optimum balance between two divergent groups of traits i.e. root yield traits (root morphometric traits and crude fiber content and root textural quality traits (starch content and SFR to develop superior genotypes with better yield and quality.

  13. Root lattices and quasicrystals

    Science.gov (United States)

    Baake, M.; Joseph, D.; Kramer, P.; Schlottmann, M.

    1990-10-01

    It is shown that root lattices and their reciprocals might serve as the right pool for the construction of quasicrystalline structure models. All noncrystallographic symmetries observed so far are covered in minimal embedding with maximal symmetry.

  14. Roots and routes

    DEFF Research Database (Denmark)

    Christensen, Ann-Dorte; Jensen, Sune Qvotrup

    2011-01-01

    This article is about transnational migrants, how they construct belonging to ‘new’ places where they have arrived, and how the feelings of belonging to their places of origin change when they go back. The theoretical part of the article outlines the relationship between migration and belonging...... arguing that there is a dynamic interplay between roots and routes in people's lives. The empirical point of departure is narratives about roots and routes by ethnic minorities settled in Aalborg East, an underprivileged neighbourhood in northern Denmark. One of the main findings is a gap between....... A somewhat paradoxical finding is that it appears to be more difficult for transnational migrants to maintain their roots in the country of origin when they go back than it was to establish new roots in the host country...

  15. Root morphology and mycorrhizal symbioses together shape nutrient foraging strategies of temperate trees.

    Science.gov (United States)

    Chen, Weile; Koide, Roger T; Adams, Thomas S; DeForest, Jared L; Cheng, Lei; Eissenstat, David M

    2016-08-02

    Photosynthesis by leaves and acquisition of water and minerals by roots are required for plant growth, which is a key component of many ecosystem functions. Although the role of leaf functional traits in photosynthesis is generally well understood, the relationship of root functional traits to nutrient uptake is not. In particular, predictions of nutrient acquisition strategies from specific root traits are often vague. Roots of nearly all plants cooperate with mycorrhizal fungi in nutrient acquisition. Most tree species form symbioses with either arbuscular mycorrhizal (AM) or ectomycorrhizal (EM) fungi. Nutrients are distributed heterogeneously in the soil, and nutrient-rich "hotspots" can be a key source for plants. Thus, predicting the foraging strategies that enable mycorrhizal root systems to exploit these hotspots can be critical to the understanding of plant nutrition and ecosystem carbon and nutrient cycling. Here, we show that in 13 sympatric temperate tree species, when nutrient availability is patchy, thinner root species alter their foraging to exploit patches, whereas thicker root species do not. Moreover, there appear to be two distinct pathways by which thinner root tree species enhance foraging in nutrient-rich patches: AM trees produce more roots, whereas EM trees produce more mycorrhizal fungal hyphae. Our results indicate that strategies of nutrient foraging are complementary among tree species with contrasting mycorrhiza types and root morphologies, and that predictable relationships between below-ground traits and nutrient acquisition emerge only when both roots and mycorrhizal fungi are considered together.

  16. Root morphology and mycorrhizal symbioses together shape nutrient foraging strategies of temperate trees

    Science.gov (United States)

    Chen, Weile; Koide, Roger T.; Adams, Thomas S.; DeForest, Jared L.; Cheng, Lei; Eissenstat, David M.

    2016-01-01

    Photosynthesis by leaves and acquisition of water and minerals by roots are required for plant growth, which is a key component of many ecosystem functions. Although the role of leaf functional traits in photosynthesis is generally well understood, the relationship of root functional traits to nutrient uptake is not. In particular, predictions of nutrient acquisition strategies from specific root traits are often vague. Roots of nearly all plants cooperate with mycorrhizal fungi in nutrient acquisition. Most tree species form symbioses with either arbuscular mycorrhizal (AM) or ectomycorrhizal (EM) fungi. Nutrients are distributed heterogeneously in the soil, and nutrient-rich “hotspots” can be a key source for plants. Thus, predicting the foraging strategies that enable mycorrhizal root systems to exploit these hotspots can be critical to the understanding of plant nutrition and ecosystem carbon and nutrient cycling. Here, we show that in 13 sympatric temperate tree species, when nutrient availability is patchy, thinner root species alter their foraging to exploit patches, whereas thicker root species do not. Moreover, there appear to be two distinct pathways by which thinner root tree species enhance foraging in nutrient-rich patches: AM trees produce more roots, whereas EM trees produce more mycorrhizal fungal hyphae. Our results indicate that strategies of nutrient foraging are complementary among tree species with contrasting mycorrhiza types and root morphologies, and that predictable relationships between below-ground traits and nutrient acquisition emerge only when both roots and mycorrhizal fungi are considered together. PMID:27432986

  17. Assessment of the bacteria reduction in the infected root canal irradiated with diode laser; Avaliacao da reducao bacteriana em conduto radicular infectado e irradiado com laser de diodo. Estudo in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Radaelli, Claudia Amaral Rabello de Mello

    2002-07-01

    High success rates are achieved in conventional endodontic treatment of vital pulp teeth. However, in cases of non-vital pulp, a decrease in the rate of success occurs due to difficulties in achieving a complete disinfection of the root canals system. Some bacteria, such as Enterococcus faecalis, are frequently found in cases of endodontic treatment failure due to their high resistance to the conventional endodontic treatment. The aim of this study was to evaluate the efficiency of a high power diode laser irradiation in bacterial reduction of contaminated canals associated with dressing compose by calcium hydroxide paste propylene glycol and camphorated paramonochlorophenol. Eighty-two root canals were infected in vitro with Enterococcus faecalis in a concentration of 1x10{sup 8} CFU/ml. Specimens were high intensity irradiated with a diode laser model Opus 10, at a wavelength of 830 nm. Two different parameters were employed in continuous mode: 3 W and 2,5 W with a 360 {mu}m optical fiber at an angle of approximately 5 degrees respect to the dentine surface during 5 seconds, in 4 applications, with 20 seconds intervals among them. After these proceedings specimens were vortexed in peptone water and dilutions performed. Aliquots of the dilution were plated on m-Enterococcus agar, incubated, and the Colonies Forming Units (CFU) of ali groups was counted. The results showed a significant reduction of bacteria on ali groups after laser irradiation. A high reduction rate was achieved: 98.5% immediately after the laser irradiation; 48 hours after, the reduction was of 96,73% and, finally, a 100% reduction was achieved through the combination of laser irradiation and a long lasting dressing of calcium hydroxide paste, propylene glycol and camphorated paramonochlorophenol. High rates of bacteria reduction were achieved using the parameter of 3 W in continuous mode with the power of 2,9473 KW/cm{sup 2}. The temperature was monitored with a K-pipe thermocouple placed at

  18. Causas referidas para o desenvolvimento de úlceras em pés de pessoas com diabetes mellitus Causas referidas al desarrollo de úlceras en piés de personas con diabetes mellitus Root causes for the development of foot ulcers of people with diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Isabela dos Santos Martin

    2012-01-01

    Full Text Available OBJETIVO: Analisar as causas referidas na etiologia das úlceras em pés de pessoas com Diabetes mellitus (DM. MÉTODOS: Estudo seccional, quantitativo, realizado no Ambulatório de Diabetes de um Hospital Universitário em Ribeirão Preto - SP. Os dados foram coletados com instrumento estruturado e exame físico dos pés de amostra de 30 pacientes diabéticos. RESULTADOS: Amostra com idade média de 57,5 anos, predominância do sexo masculino e baixa escolaridade; 90% possuíam DM tipo 2, de longa duração e mal controlado; obesidade/sobrepeso em 77% e insensibilidade plantar em 93,3%. A região metatarsiana foi o local de úlcera referido com maior frequência, e a causa foi a calosidade. CONCLUSÃO: as causas referidas envolvidas na etiologia das úlceras correspondem, de forma direta ou indireta, a fatores extrínsecos que podem ser prevenidos com cuidados básicos e de baixo custo. A insensibilidade plantar, fator fundamental desencadeador das úlceras, no entanto não foi reconhecida pelas pessoas.OBJETIVO: Analizar las causas referidas en la etiología de las úlceras en piés de personas con Diabetes mellitus (DM. MÉTODOS: Estudio seccional, cuantitativo, realizado en el consultorio externo de Diabetes de un Hospital Universitario en Ribeirão Preto - SP. Los datos fueron recolectados con un instrumento estructurado y examen físico de los piés de una muestra de 30 pacientes diabéticos. RESULTADOS: muestra conformada por personas con una edad promedio de 57,5 años, predominio del sexo masculino y baja escolaridad;el 90% poseían DM tipo 2, de larga duración y mal controlado; obesidad/sobre peso en el 77% e insensibilidad plantar en el 93,3%. La región metatarsiana fue referida, con mayor frecuencia, como el lugar de la úlcera y la causa fue la callosidad. CONCLUSIÓN: Las causas que están involucradas en la etiología de las úlceras corresponden, de forma directa o indirecta, a factores extrínsecos que pueden ser prevenidos

  19. Cold storage of rooted and non-rooted carnation cuttings

    African Journals Online (AJOL)

    USER

    2010-06-14

    Jun 14, 2010 ... The specific objectives of this study were to evaluate the effect of storage duration on the survival rate of rooted cuttings and to determine the rooting and survival rates of non-rooted cuttings for two standard carnation cultivars (that is., Dianora and Vittorio). The survival rates of rooted cuttings showed ...

  20. Sesquiterpene lactones and inulin from chicory roots : extraction, identification, enzymatic release and sensory analysis

    NARCIS (Netherlands)

    Leclercq, E.

    1992-01-01

    Chicory ( <em>Cichorium intybusem> L.) is one of the many species of the family Compositae. Chicory has been cultivated for the production of leaves or chicons, which have been used as a vegetable since approximately 300 BC, and for its roots, which can be used as a

  1. Towards isolation of the tomato root-knot nematode resistance gene MI via positional cloning

    NARCIS (Netherlands)

    Daelen, van R.A.J.J.

    1995-01-01

    Root-knot nematodes of the genus <em>Meloidogyne areem> severe pathogens of plants and worldwide they cause damage to many economically important crops like potato, rice, cotton, and tomato. So the control of nematodes and the protection of plants against nematode damage are

  2. Diversity of an ectomycorrhizal fungal community studied by a root tip and total soil DNA approach

    NARCIS (Netherlands)

    Landeweert, R.; Leeflang, P.; Smit, E.; Kuyper, T.W.

    2005-01-01

    Molecular methods based on soil DNA extracts are increasingly being used to study the fungal diversity of ectomycorrhizal (EM) fungal communities in soil. Contrary to EM root tip identification, the use of molecular methods enables identification of extramatrical mycelia in soil. To compare fungal

  3. Root water uptake under non-uniform transient salinity and water stress

    NARCIS (Netherlands)

    Homaee, M.

    1999-01-01

    The study described in this thesis focuses on the quantitative understanding of water uptake by roots under separate and combined salinity and water stresses. The major difficulty in solving <em>Richards'> equation stems from the lack of a sink term function that adequately

  4. Relationships between root diameter, root length and root branching along lateral roots in adult, field-grown maize.

    Science.gov (United States)

    Wu, Qian; Pagès, Loïc; Wu, Jie

    2016-03-01

    Root diameter, especially apical diameter, plays an important role in root development and function. The variation in diameter between roots, and along roots, affects root structure and thus the root system's overall foraging performance. However, the effect of diameter variation on root elongation, branching and topological connections has not been examined systematically in a population of high-order roots, nor along the roots, especially for mature plants grown in the field. A method combining both excavation and analysis was applied to extract and quantify root architectural traits of adult, field-grown maize plants. The relationships between root diameter and other root architectural characteristics are analysed for two maize cultivars. The basal diameter of the lateral roots (orders 1-3) was highly variable. Basal diameter was partly determined by the diameter of the bearing segment. Basal diameter defined a potential root length, but the lengths of most roots fell far short of this. This was explained partly by differences in the pattern of diameter change along roots. Diameter tended to decrease along most roots, with the steepness of the gradient of decrease depending on basal diameter. The longest roots were those that maintained (or sometimes increased) their diameters during elongation. The branching density (cm(-1)) of laterals was also determined by the diameter of the bearing segment. However, the location of this bearing segment along the mother root was also involved - intermediate positions were associated with higher densities of laterals. The method used here allows us to obtain very detailed records of the geometry and topology of a complex root system. Basal diameter and the pattern of diameter change along a root were associated with its final length. These relationships are especially useful in simulations of root elongation and branching in source-sink models. © The Author 2016. Published by Oxford University Press on behalf of the Annals

  5. The "Green" Root Beer Laboratory

    Science.gov (United States)

    Clary, Renee; Wandersee, James

    2010-01-01

    No, your students will not be drinking green root beer for St. Patrick's Day--this "green" root beer laboratory promotes environmental awareness in the science classroom, and provides a venue for some very sound science content! While many science classrooms incorporate root beer-brewing activities, the root beer lab presented in this article has…

  6. Citotoxicity of food dyes sunset yellow (E-110, bordeaux red (E-123, and tatrazine yellow (E-102 on Allium cepa L. root meristematic cells Citotoxicidade dos corantes alimentares amarelo crepúsculo (E-110, vermelho bordeaux (E-123 e amarelo tartrazina (E-102 em células meristemáticas de raízes de Allium cepa L.

    Directory of Open Access Journals (Sweden)

    Keiva Maria Silva Gomes

    2013-03-01

    Full Text Available The objective of this study was to evaluate the cytotoxic effect of the food dyes sunset yellow, bordeaux red, and tartrazine yellow on the cellular cycle of Allium cepa L. Each dye was evaluated at the doses of 0.4 and 4.0 mL, at the exposure times of 24 and 48 hours in root tip cells of Allium cepa L. Slides were prepared and cells were analyzed during the whole cell cycle for cellular aberrations totaling 5,000 total cells for each dose evaluated. The mitotic index was calculated, and statistical analysis was performed using the Chi-squared test (p Este estudo teve por objetivo avaliar o efeito citotóxico dos corantes alimentares amarelo crepúsculo, vermelho bordeaux e amarelo tartrazina sobre o ciclo celular de Allium cepa L. Cada corante foi avaliado nas doses de 0,4 e 4,0 mL, nos tempos de exposição de 24 e 48 horas, em células de pontas de raízes de Allium cepa L. Prepararam-se lâminas e analisaram-se células, em todo o ciclo celular, e a presença de aberrações celulares, totalizando 5.000 células para cada dose avaliada. Foi calculado o índice mitótico e a análise estatística foi feita por meio do teste quiquadrado (p < 0,05. Os resultados mostraram que os três corantes, nas doses e tempos de exposição avaliados, foram citotóxicos às células do sistema-teste utilizado. Estudos adicionais de citotoxicidade devem ser conduzidos para se somar a estes resultados e, assim, avaliar, com propriedade, a ação destes três corantes em nível celular.

  7. Canine malignant peripheral nerve sheath tumor involving nerve roots of the third lumbar spinal cord segmentTumor maligno da bainha de nervo periférico envolvendo raízes nervosas do terceiro segmento medular lombar em um cão

    Directory of Open Access Journals (Sweden)

    Elisângela Olegário da Silva

    2012-12-01

    Full Text Available Malignant peripheral nerve sheath tumors (MPNST involving spinal nerve roots are uncommon in dogs. A nine-year old, intact, mixed-breed dog, demonstrated clinical signs of incoordination in the pelvic limbs and micturition for approximately one week. Clinical examination revealed proprioceptive deficits and bilateral patellar hyperreflexia. During exploratory celiotomy a mass was observed adhered to the lumbar vertebral segments. Medical therapy was initiated, but neurological signs were progressive, and the owner opted for euthanasia. Gross examination showed that the mass in the abdominal cavity was attached to the lumbar segments L3 and L4, causing bone lysis in L3, but showed no tumor invasion into the spinal canal. Microscopic features were characterized by prominent proliferation of ovoid and fusiform cells with poorly defined cytoplasm arranged in interlacing bundles and concentric whorls. The cells were embedded in a delicate to moderate collagenous stroma and moderate anisokariose and high mitotic activity were noted. The immunohistochemical assay showed positive staining for GFAP, S-100 protein and vimentin, and negative staining for factor VIII, ?-actin and citokeratine. The definitive diagnosis of malignant peripheral nerve sheath tumor was made on the basis of the histological and immunohistochemical findings. Tumores malignos da bainha de nervo periférico (TMBNP em raízes nervosas espinhais são incomuns em cães. Relata-se o caso de um cão, sem raça definida, nove anos de idade, não castrado, com histórico de incoordenação em membros pélvicos e retenção urinária há aproximadamente uma semana. Ao exame clínico constatou-se déficit proprioceptivo e hiperreflexia patelar bilaterais. Durante a celiotomia exploratória constatou-se uma massa intensamente vascularizada e aderida aos segmentos vertebrais lombares. Estabeleceu-se plano terapêutico e o animal foi tratado com fluidoterapia, anti-inflamatório e analg

  8. Herdabilidade e correlações genotípicas entre caracteres de folhagem e sistema radicular em famílias de cenoura, cultivar Brasília Heritability and genotypic correlation among leaf and root traits in carrot, cultivar Brasilia progenies

    Directory of Open Access Journals (Sweden)

    José Carlos da S Alves

    2006-09-01

    Full Text Available O presente trabalho foi implementado com o objetivo de estimar parâmetros genéticos para os seguintes caracteres de importância para o melhoramento genético da cenoura: número de folhas por planta (NFP, comprimento da maior folha na planta (CF, comprimento da raiz (CR, peso da raiz (PR, diâmetro da raiz (DR e diâmetro do xilema (DX. Este experimento foi conduzido em condições de campo durante o verão de 1999/2000, sendo utilizadas 69 famílias de meios-irmãos derivadas da cultivar Brasília. As progênies foram cultivadas em delineamento de blocos ao acaso com duas repetições e parcelas de 2 m², com quatro linhas de 20 plantas cada. Os valores observados para coeficiente de variação genética oscilaram de 4,8 a 10,9. Os valores calculados de herdabilidade no sentido amplo (h a² foram medianamente altos para todos os caracteres, com uma variação observada entre 29,9% (para DR e 77,6% (para CF. Foram também obtidas estimativas de valores de correlações fenotípicas, genotípicas e ambientais entre os caracteres avaliados, com destaque para a magnitude do valor da correlação genotípica entre PR e DR, de 0,85. Correlação genotípica negativa foi observada entre CR e DX indicando a possibilidade de desenvolver, a partir de populações derivadas de 'Brasília', novas cultivares com arquitetura de raiz mais adequada para a produção de mini-cenouras ("baby-carrots". Valores de correlações ambientais negativos e altos foram observados entre PR e DX e entre DR e DX. Este conjunto de informações representa uma importante base de dados genéticos com implicações diretas na otimização do processo de seleção dentro de populações de cenoura derivadas da cultivar Brasília.The present work was conducted aiming to estimate genetic parameters associated with traits of importance to carrot breeding, including: number of leaves per plant (NL, length of the longest leaf in the plant (LL, root length (RL, root weight (RW

  9. In vivo evaluation of the sealing ability of two endodontic sealers in root canals exposed to the oral environment for 45 and 90 days Avaliação, in vivo, da capacidade de selamento de dois cimentos endodônticos em canais radiculares expostos ao meio bucal por 45 e 90 dias

    Directory of Open Access Journals (Sweden)

    Patrícia Maria Poli Kopper

    2006-01-01

    Full Text Available This in vivo study evaluated the sealing ability of a resin-based sealer (AH Plus and a zinc oxide-eugenol sealer (Endofill in dogs' teeth, exposed to the oral environment for 45 and 90 days. Forty eight lower incisors from 8 dogs were endodonticaly treated. A stratified randomization determined the sealer use in each root canal. All canals were filled using the lateral condensation technique. The excess filling material at the cervical portion of the root canal was sectioned, leaving a 10-mm obturation length inside the canal. Teeth were provisionally sealed with glass ionomer cement for 24 h and the canals were exposed to the oral environment for either 45 or 90 days. Therefore, the experimental groups were as follows: A45- AH Plus for 45 days; A90- AH Plus for 90 days; E45- Endofill for 45 days; and E90- Endofill for 90 days (n=12. After the experimental period, the dogs were killed and the lower jaw was removed. The incisors were extracted and the roots were covered with two coats of nail varnish. The teeth were immersed in India ink for 96 h and submitted to diaphanization. Dye leakage (in mm was measured using stereomicroscopy (10x magnification. The results were statistically analyzed using two-way ANOVA and Tukey test for multiple comparisons (á = 0.05. Group E90 (2.03±0.94 showed significantly higher mean leakage value than all other groups (pEste estudo in vivo avaliou a capacidade de selamento de um cimento endodôntico resinoso (AH Plus e um a base de óxido de zinco eugenol (Endofill, em dentes de cães, expostos ao meio bucal, por 45 e 90 dias. Foi realizado o tratamento endodôntico de 48 incisivos inferiores de 8 cães. Uma randomização estratificada determinou o tipo de cimento a ser usado em cada canal que foram tratados pela técnica de condensação lateral. Após a obturação, o excesso de material obturador, na porção cervical do canal radicular foi seccionado, restando o comprimento de 10 mm no interior do canal

  10. "In vitro" evaluation of the apical sealing of root canals obturated with different techniques Avaliação "in vitro" do selamento apical dos canais radiculares em função de diferentes técnicas de obturação

    Directory of Open Access Journals (Sweden)

    Viviane Haiub Brosco

    2003-09-01

    Full Text Available The purpose of this study was to compare the apical sealing of root canals obturated with different techniques. One hundred-six human mandibular incisors were submitted to instrumentation by means of the step-back technique. After instrumentation, one hundred teeth received an impermeable coating on the external surfaces of the crown and root (except for the area nearby the apical foramen. Afterwards, they were divided in five groups containing twenty elements each, according to the obturation technique employed: 1. lateral condensation with Kerr file; 2. continuous wave of condensation technique with System B; 3. thermoplasticized injectable gutta-percha technique with the Ultrafil system; 4. mechanically thermoplasticized gutta-percha with the JS Quick-Fill system and 5. thermoplasticized gutta-percha associated to a master cone with the Microseal system. The six remaining teeth were employed as negative and positive controls. After obturation, the access cavities were sealed and the teeth were immersed in aqueous 2% methylene blue dye for 72 hours at 37ºC. After that, the teeth were longitudinally sectioned and the apical microleakage was evaluated in a stereomicroscope. The Microseal system presented the best apical sealing ability, followed by System B, JS Quick-Fill, Ultrafil and the lateral condensation technique. The statistical analysis of the results demonstrated that: 1. the Microseal system presented an apical sealing similar to System B and better than the other groups; 2. System B presented better apical sealing than the lateral condensation technique, being similar to the other groups; and 3. the lateral condensation, Ultrafil and JS Quick-Fill groups demonstrated similar sealing ability.Este estudo teve como objetivo avaliar o selamento apical de canais radiculares obturados por diferentes técnicas. Cento e seis incisivos inferiores humanos tiveram seus canais instrumentados através da técnica escalonada regressiva. Após a

  11. Potencial de rizobactérias na promoção de crescimento e controle da podridãoradicular em alface hidropônica Potential of rhizobacteria to promote root rot growth and control in hydroponically cultivated lettuce

    Directory of Open Access Journals (Sweden)

    Matheus Aparecido Pereira Cipriano

    2013-03-01

    Full Text Available Os objetivos do trabalho foram avaliar se isolados de rizobactérias do grupo fluorescente do gênero Pseudomonas: 1 produzem metabólitos envolvidos na promoção do crescimento (AIA e HCN; 2 têm potencial para controle biológico de Pythium aphanidermatum; 3 podem promover o crescimento de plantas de alface cultivadas em sistema hidropônico; 4 e verificar se há correspondência nas interações in vitro e in vivo desses microrganismos. Com esses objetivos, placas de Petri contendo meio de cultura ágar-água, com ou sem Pythium aphanidermatum, receberam sementes prégerminadas de alface tratadas com os isolados de rizobactérias. Os comprimentos do hipocótilo e da radícula foram medidos cinco ou sete dias após a incubação a 28 ºC. Dois experimentos foram conduzidos em casa de vegetação com plantas de alface hidropônica e 17 isolados bacterianos. As unidades experimentais receberam a suspensão dos isolados de Pseudomonas spp. e, uma semana depois, a suspensão de zoósporos de P. aphanidermatum. Avaliaram-se o escurecimento das raízes e a massa de matéria seca da parte aérea e das raízes das plantas. Os isolados produzem metabólitos que beneficiam o crescimento de alface mesmo na presença do patógeno. Os isolados LP15, LP25, LP28, LP44 e LP47 reduziram os danos causados por P. aphanidermatum nos experimentos realizados in vitro e in vivo. Este é o primeiro estudo que demonstrou que rizobactérias obtidas de solos brasileiros têm potencial para promover o controle biológico de P. aphanidermatum e o crescimento de plantas de alface em sistemas hidropônicos.The aims of this study were to evaluate whether rhizobacteria with hydroponic lettuce plants and 17 bacterial isolates. The experimental isolates of the fluorescent group of the genus Pseudomonas: 1 units received Pseudomonas spp. isolate suspension and, one week later, produce metabolites involved in growth promotion (AIA and HCN; P. aphanidermatum zoospore suspension

  12. Crescimento de raízes de biótipos de capim-arroz resistente e suscetível ao quinclorac em competição Root growth of Echinochloa biotypes resistant and susceptible to quinclorac under competition

    Directory of Open Access Journals (Sweden)

    L.O.B. Schuch

    2008-01-01

    Full Text Available Objetivou-se com este trabalho avaliar o potencial competitivo de biótipos de capim-arroz resistente e suscetível ao quinclorac no ambiente do solo, como forma de inferir qual deles possui maior potencial de extração de recursos. O experimento foi instalado em casa de vegetação, constando de diferentes populações de plantas dos biótipos de capim-arroz resistente (R e suscetível (S ao herbicida quinclorac, oriundos da região arrozeira de Itajaí/SC. Foi estabelecida uma planta do biótipo de capim-arroz considerado como tratamento, no centro da unidade experimental; na periferia, foram semeadas dez sementes do biótipo oposto ao do tratamento central, sendo posteriormente estabelecido o número de plantas de acordo com o tratamento (0, 1, 2, 3, 4 ou 5 plantas. O delineamento experimental foi o completamente casualizado, em esquema fatorial 2 x 6, com quatro repetições. Aos 40 DAE, foram avaliados o comprimento, volume, massa fresca, massa seca e conteúdo de água das raízes das plantas. Os dados obtidos foram submetidos à análise de variância e, em caso de significância, efetuou-se o teste de Duncan, para avaliar o efeito do aumento na população de plantas, e o teste da Diferença Mínima Significativa (DMS, para avaliar diferenças entre biótipo resistente e suscetível, além da matriz de correlação. A imposição de competição no ambiente das raízes pode afetar mais o biótipo resistente ao quinclorac que o suscetível, sendo esta característica essencial para a sobrevivência do biótipo, principalmente sob escassez do recurso.The objective of this work was to evaluate the competitive potential of Echinochloa plants resistant and susceptible to quinclorac in the soil, to evaluate whether there are potential differences in extracting nutrients from the soil. The trial was carried out under greenhouse conditions. The treatments consisted of amounts of Echinochloa plants resistant or susceptible to quinclorac. At the

  13. Towards Novel Techniques for Root Phenotyping Using GPR

    Science.gov (United States)

    Kobylinski, C.; Neely, H.; Everett, M. E.; Hays, D. B.; Lewis, K.

    2017-12-01

    The ability to phenotype roots in situ would provide information for carbon sequestration potential through increased root mass, possible water-seeking strategies by plants, and generate data for plant breeders. One technique for root phenotyping is to measure differences in soil moisture and use this data to infer root presence or absence. Current technologies for soil moisture detection include electromagnetic induction and neutron moisture meters; however, ground penetrating radar (GPR) has been suggested to monitor root phenotypes. The objective of this study is to use GPR as a novel technique for detecting roots and classifying root phenotypes based on the detection of differences in dielectric permittivity in response to changes in soil water content. The study will be conducted at two sites in Texas: Thrall, TX (Burleson clay) and Lubbock, TX (Olton clay loam). Three root types will be investigated: fibrous (grain sorghum), tap root (cowpea), and mixed (9-species). Data will be collected along a 10 m linear transect in each plot with a PulseEkko GPR bi-static unit operating at a radio frequency of 500 MHz. Additionally, an EM38-MK2 survey will be performed along each transect. Soil surface moisture readings will be collected with a ML3 ThetaProbe soil moisture sensor and a neutron moisture meter will be used to obtain soil moisture measurements down to 1.2 m. Measurements will be collected every two weeks throughout the growing season. Soil properties including particle size distribution, cation exchange capacity, and bulk density will also be measured. GPR's ability to distinguish root types across soils will be assessed.

  14. Seasonal changes in nitrogen availability, and root and microbial uptake of 15N13C9-phenylalanine and 15N-ammonium <em>in situem> at a temperate heath

    DEFF Research Database (Denmark)

    Andresen, Louise C.; Michelsen, Anders; Jonasson, Sven Evert

    2011-01-01

    In the plant biosynthesis of secondary compounds, phenylalanine is a precursor of condensed tannins. Tannins are deposited into the soil in plant root exudates and dead plant material and have been suggested to precipitate some soil nutrients and hence reduce nutrient availability for plants. Free...... amino acid,inorganic and microbial N concentration during the growing season was investigated in an ecosystem with a natural tannin chemosphere. The influence of tannins on the uptake of nitrogen in plants and microbes was followed by injecting tannic acid (TA), ammonium-15N and phenylalanine-15N/13C9...

  15. Metagenomics at Grass Roots

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 22; Issue 3. Metagenomics at Grass Roots. Sudeshna ... benefit human health, agriculture, and ecosystemfunctions. This article provides a brief history of technicaladvances in metagenomics, including DNA sequencing methods,and some case studies.

  16. (Lamiaceae) root extracts

    African Journals Online (AJOL)

    Purpose: To evaluate the larvicidal, nematicidal, antifeedant, and antifungal effects of 10 solvent extracts of Mentha spicata root. Methods: Ten solvent extracts were investigated for their total flavonoid and phenolic content and screened for larvicidal, nematicidal, antifeedant, and antifungal activities. The total phenolic ...

  17. Avaliação nutricional e desempenho da silagem de raiz de mandioca contendo ou não soja integral em dietas para suínos - doi: 10.4025/actascianimsci.v32i2.8055 Nutritional evaluation and performance of cassava root silage with or without whole soybean in swine diets - doi: 10.4025/actascianimsci.v32i2.8055

    Directory of Open Access Journals (Sweden)

    Paulo Levi Oliveira Carvalho

    2010-07-01

    Full Text Available Dois experimentos foram conduzidos para determinar o valor nutritivo e o desempenho de suínos nas fases de crescimento e período total, alimentados com dietas formuladas com silagem de raiz de mandioca contendo ou não soja integral. No primeiro, foram utilizados 15 suínos, distribuídos em delineamento inteiramente casualizado em parcelas (animais subdivididas no tempo (subparcelas, totalizando cinco tratamentos e seis repetições. Os alimentos avaliados foram silagem de raiz de mandioca (MA, silagem de raiz de mandioca com inoculante (MI, silagem de raiz de mandioca + soja integral (MS e silagem de raiz de mandioca + soja integral com inoculante (MSI. As silagens apresentaram bons valores nutritivos e o uso de inoculante não foi efetivo para melhorar a digestibilidade dos nutrientes. No segundo, foram utilizados 36 suínos mestiços, distribuídos em um delineamento inteiramente casualizado, com restrição na casualização para duas classes de peso inicial, com três tratamentos, seis repetições e dois animais por unidade experimental. Os tratamentos consistiram em ração-testemunha à base de milho e farelo de soja (RT e outras duas com substituição total do milho por MA e MS. Para fase de crescimento, a conversão alimentar melhorou com o uso das silagens. Conclui-se que as silagens de mandioca, contendo ou não soja integral, apresentam bons valores nutritivos e podem substituir totalmente o milho na ração de suínos nas fases de crescimento e período total. alimentos alternativos; amido; desempenho; digestibilidade; valores energéticosTwo experiments were carried out to determine the nutritional value and performance of growing and total-period swine fed cassava root silage with or without whole soybean. In the first group, 15 crossbred swine were used, in a completely randomized design with parcels (animals subdivided in time (subparcels with five treatments and six replications. The study evaluated cassava root silage (CA

  18. Produção de matéria seca, crescimento radicular e absorção de cálcio, fósforo e alumínio por coffea canephora e coffea arabica sob influência da atividade do alumínio em solução Dry matter production, root growth and calcium, phosphorus and aluminum absorption by coffea canephora and coffea arabica under influence of aluminum activity in solution

    Directory of Open Access Journals (Sweden)

    Edson Marcio Mattiello

    2008-02-01

    Full Text Available Este trabalho teve como objetivo avaliar a produção de matéria seca, o crescimento radicular e a absorção e distribuição do Ca, P e Al nas folhas, no caule e nas raízes de dois clones de café conilon (Coffea canephora (Mtl 25 e Mtl 27 e de uma variedade de café Catuaí Amarelo (Coffea arabica, cultivados em solução nutritiva com atividade crescente de Al3+. As plantas foram cultivadas em vasos com capacidade para 5 L, contendo solução nutritiva de Hoagland & Arnon, modificada. Após oito dias de adaptação, as plantas foram submetidas a concentrações de Al de 0, 500, 1.000 e 2.000 µmol L-1, que corresponderam a atividades de Al3+ em solução, estimadas pelo software GEOCHEM, de 20,68, 50,59, 132,9 e 330,4 µmol L-1, respectivamente. Foram determinados os teores de Ca, Al e P na planta. O sistema radicular foi separado, para determinação da área e do comprimento. A variedade Catuaí Amarelo (Coffea arabica apresentou-se menos sensível ao Al3+, quando comparada aos clones de conilon (Coffea canephora. O clone de conilon Mtl 25 foi menos sensível ao Al3+ em relação ao Mtl 27. O aumento da atividade de Al3+ promoveu redução nos teores de P e Ca nas folhas e raízes do cafeeiro, especialmente nos clones Mtl 25 e Mtl 27. O acúmulo de Al no sistema radicular e a restrição do transporte para a parte aérea são importantes fatores na tolerância de plantas ao Al3+.This study had the objective of evaluating the dry matter production, root growth, and the absorption and distribution of Ca, P and Al in the leaves, stem and roots of two Conilon (Coffea canephora coffee clones (Mtl 25 and Mtl 27 and the coffee variety Catuaí Amarelo (Coffea arabica grown in nutrient solution with increasing Al3+ activity. The plants were cultivated in 5 L pots, containing modified Hoagland & Arnold nutrient solution. After eight days of adaptation, the plants were subjected to Al concentrations of 0, 500, 1.000 and 2.000 mol L-1, which

  19. Introduction to the ROOT System

    CERN Multimedia

    CERN. Geneva

    2008-01-01

    Introduction to the ROOT data handling system. ROOT is used in some for or another by all LHC experiments and will be used by all for final data analysis. The introduction gives an overview of the system. Prerequisite knowledge: C++

  20. Variation in root wood anatomy

    NARCIS (Netherlands)

    Cutler, D.F.

    1976-01-01

    Variability in the anatomy of root wood of selected specimens particularly Fraxinus excelsior L. and Acer pseudoplatanus L. in the Kew reference microscope slide collection is discussed in relation to generalised statements in the literature on root wood anatomy.

  1. Análise em microscopia eletrônica de varredura de superfícies radiculares antes e após raspagem e condicionamento com ácido cítrico e EDTA: um estudo "in vitro" Scanning electron microscopy analysis on root surfaces before and after scalling and citric acid and EDTA conditioning: an "in vitro" study

    Directory of Open Access Journals (Sweden)

    Fabio Valverde Rodrigues Bastos Neto

    2003-03-01

    Full Text Available A superfície radicular mais benéfica para a regeneração do periodonto ainda não está estabelecida. Visando uma busca por esta superfície este trabalho utilizou 14 dentes de humanos recém extraídos, seccionados em corpos de prova e tratados com raspagem e condicionamentos com ácido cítrico e EDTA e analisados em microscopia eletrônica de varredura. As superfícies hígidas apresentavam grânulos regulares provenientes de fibras do ligamento periodontal rompidas na exodontia. As superfícies contaminadas apresentavam massas de cálculo aderidas e áreas de reabsorção cementária. Quando raspadas com curetas manuais as superfícies foram cobertas por "smear layer". O condicionamento com ácido cítrico foi eficiente na remoção total do "smear layer", assim como o condicionamento com EDTA gel, e o condicionamento com EDTA líquido não teve efetividade total na sua remoção. Tomando como padrão as superfícies hígidas, conclui-se que os tratamentos que mais se aproximam do ideal são a raspagem e condicionamento com ácido cítrico e EDTA gel sem diferenças entre si.The most benefic root surface for periodontal regeneration has not been known. Looking for this surface this study used 14 human teeth prepared as testing corpus and treated with scaling and conditioning with citric acid and EDTA, and analysed in scanning electron microscopy. The health teeth surfaces showed regular grains representing broken periodontal ligament fibers by exodontics. The contaminated surfaces showed calculus masses, very porous, and resorption areas. When scalled with hand curettes the surfaces was covered by smear layer. The citric acid conditioning was efficient on smear layer removal, as well as the EDTA gel, and the EDTA liquid solution was not efficient on smear removal.

  2. Diversity of an ectomycorrhizal fungal community studied by a root tip and total soil DNA approach.

    Science.gov (United States)

    Landeweert, Renske; Leeflang, Paula; Smit, Eric; Kuyper, Thom

    2005-01-01

    Molecular methods based on soil DNA extracts are increasingly being used to study the fungal diversity of ectomycorrhizal (EM) fungal communities in soil. Contrary to EM root tip identification, the use of molecular methods enables identification of extramatrical mycelia in soil. To compare fungal diversity as determined by root tip identification and mycelial identification, six soil samples were analysed. Root tips were extracted from the six samples and after amplification, the basidiomycete diversity on the root tips was analysed by denaturing gradient gel electrophoresis (DGGE). The soil from the six samples was sieved, total soil DNA was extracted and after amplification, the basidiomycete diversity in the soil fractions was analysed by DGGE. Fourteen different bands were excised from the DGGE gel and sequenced; fungal taxon names could be assigned to eight bands. Out of a total of 14 fungal taxa detected in soil, 11 fungal taxa were found on root tips, of which seven were EM fungal taxa. To examine whether the sieving treatment would affect EM species diversity, two different sieve mesh sizes were used and in addition, the organic soil fraction was analysed separately. DGGE analysis showed no differences in banding pattern for the different soil fractions. The organic fraction gave the highest DGGE band intensities. This work demonstrates that there is a high correspondence between basidiomycete diversity detected by molecular analysis of root tips and soil samples, irrespective of the soil fraction being analysed.

  3. Adição de ácido cítrico potencializa a ação de ácidos húmicos e altera o perfil protéico da membrana plasmática em raízes de milho Citric acid addition improve humic acids action and change proteins profile from plasma membrane of maize roots

    Directory of Open Access Journals (Sweden)

    Janaína Aparecida Hottz Rima

    2011-04-01

    Full Text Available A promoção do crescimento vegetal pelos ácidos húmicos tem sido atribuída a ações similares a hormônios, devido à promoção do desenvolvimento e proliferação das raízes, resultando numa absorção mais eficiente de água e nutrientes. O objetivo deste trabalho foi analisar as mudanças na arquitetura radicular em plântulas de milho e no perfil de proteínas da membrana plasmática (MP promovidas pelo tratamento com ácidos húmicos (AH isolados de vermicomposto (20mg C L-1. O efeito da adição de ácido cítrico (AC, importante ácido orgânico presente nos exudados radiculares, sobre a bioatividade destes AH também foi investigada. Foram analisados o comprimento da raiz principal, o número de sítios de mitose, o número e comprimento de raízes laterais e a área radicular total. Para a análise do perfil protéico, vesículas da MP de células de raízes foram obtidas por fracionamento celular e as proteínas analisadas por eletroforese uni (1D e bidimensional (2D. Observou-se que a adição de AC (0,005mM aos AH estimularam a promoção do crescimento das raízes laterais (126%, da área radicular (58% e do número de raízes laterais (55% em relação às plantas controle. A atividade da bomba de H+ da membrana plasmática, analisada como marcador bioquímico de indução do mecanismo do crescimento ácido, também foi significativamente estimulada (374% pela solução húmica suplementada com AC. O perfil protéico da MP revelou uma supressão da expressão das proteínas nesta membrana, induzida pelo tratamento com AH e, na presença de AC, esse efeito foi ainda mais evidente. Os resultados obtidos corroboram o mecanismo proposto para a bioatividade de AH no qual a ação de ácidos orgânicos exudados pelas plantas, tais como o AC, promove o rompimento da associação supramolecular dessas substâncias, tornando as moléculas bioativas presentes nos agregados húmicos mais acessíveis aos receptores celulares das ra

  4. Springback in root gravitropism

    Science.gov (United States)

    Leopold, A. C.; Wettlaufer, S. H.

    1989-01-01

    Conditions under which a gravistimulus of Merit corn roots (Zea mays L.) is withdrawn result in a subsequent loss of gravitropic curvature, an effect which we refer to as springback.' This loss of curvature begins within 1 to 10 minutes after removal of the gravistimulus. It occurs regardless of the presence or absence of the root cap. It is insensitive to inhibitors of auxin transport (2,3,5-triiodobenzoic acid, naphthylphthalamic [correction of naphthylphthalmaic] acid) or to added auxin (2,4-dichlorophenoxyacetic acid). Springback is prevented if a clinostat treatment is interjected to neutralize gravistimulation during germination, which suggests that the change in curvature is a response to a memory' effect carried over from a prior gravistimulation.

  5. Aquaporins and root water uptake

    Science.gov (United States)

    Water is one of the most critical resources limiting plant growth and crop productivity, and root water uptake is an important aspect of plant physiology governing plant water use and stress tolerance. Pathways of root water uptake are complex and are affected by root structure and physiological res...

  6. Analysis of the film thickness of a root canal sealer following three obturation techniques Análise da espessura da linha de cimento endodôntico em três técnicas de obturação

    Directory of Open Access Journals (Sweden)

    Gustavo André De Deus

    2003-06-01

    Full Text Available The aim of this study was to obtain a quantitative analysis of the film thickness of a root canal sealer formed after filling by three different techniques. Thirty human maxillary incisors were selected and access cavities were prepared using high-speed diamond stones and water spray. A size #15 K-Flexofile was introduced in the canal of each specimen until it was seen just at the apical foramen. The working length was determined to be 1 mm short of that position and the canals were prepared to an apical size of #45 K-Flexofile. Copious irrigation with 5.25% NaOCl (sodium hypochlorite was used during and after instrumentation. The samples were divided into three groups and obturated as follows: G1 - lateral condensation, G2 - lateral condensation with an accessory cone, and G3 - continuous wave of condensation. The samples were evaluated in the cervical, middle and apical thirds. The film thickness of the root canal sealer was measured through a microscopic evaluation. Statistical analysis was obtained using the Wilcox test. Statistical analysis showed significant differences between G3 and G1, G3 and G2 (p O objetivo deste estudo foi realizar uma análise quantitativa da espessura da linha de cimento endodôntico formada por três técnicas de obturação. Trinta incisivos centrais superiores humanos foram selecionados e acessados de modo convencional. Uma lima nº 15 K-Flexofile foi usada para a verificação da patência foraminal e para determinação do comprimento de trabalho, que foi estabelecido a 1 mm aquém do forame apical. Os canais foram preparados até a lima nº 45. Hipoclorito de sódio a 5,25% foi usado durante toda a instrumentação. Os dentes foram divididos em 3 grupos e obturados pelos seguintes critérios: G1 - condensação lateral; G2 - compressão hidraúlica e G3 - onda de condensação. Os dentes foram seccionados e analisados nos terços cervical, médio e apical. A espessura do filme de cimento foi determinada por

  7. Expressing Parallelism with ROOT

    Science.gov (United States)

    Piparo, D.; Tejedor, E.; Guiraud, E.; Ganis, G.; Mato, P.; Moneta, L.; Valls Pla, X.; Canal, P.

    2017-10-01

    The need for processing the ever-increasing amount of data generated by the LHC experiments in a more efficient way has motivated ROOT to further develop its support for parallelism. Such support is being tackled both for shared-memory and distributed-memory environments. The incarnations of the aforementioned parallelism are multi-threading, multi-processing and cluster-wide executions. In the area of multi-threading, we discuss the new implicit parallelism and related interfaces, as well as the new building blocks to safely operate with ROOT objects in a multi-threaded environment. Regarding multi-processing, we review the new MultiProc framework, comparing it with similar tools (e.g. multiprocessing module in Python). Finally, as an alternative to PROOF for cluster-wide executions, we introduce the efforts on integrating ROOT with state-of-the-art distributed data processing technologies like Spark, both in terms of programming model and runtime design (with EOS as one of the main components). For all the levels of parallelism, we discuss, based on real-life examples and measurements, how our proposals can increase the productivity of scientists.

  8. Expressing Parallelism with ROOT

    Energy Technology Data Exchange (ETDEWEB)

    Piparo, D. [CERN; Tejedor, E. [CERN; Guiraud, E. [CERN; Ganis, G. [CERN; Mato, P. [CERN; Moneta, L. [CERN; Valls Pla, X. [CERN; Canal, P. [Fermilab

    2017-11-22

    The need for processing the ever-increasing amount of data generated by the LHC experiments in a more efficient way has motivated ROOT to further develop its support for parallelism. Such support is being tackled both for shared-memory and distributed-memory environments. The incarnations of the aforementioned parallelism are multi-threading, multi-processing and cluster-wide executions. In the area of multi-threading, we discuss the new implicit parallelism and related interfaces, as well as the new building blocks to safely operate with ROOT objects in a multi-threaded environment. Regarding multi-processing, we review the new MultiProc framework, comparing it with similar tools (e.g. multiprocessing module in Python). Finally, as an alternative to PROOF for cluster-wide executions, we introduce the efforts on integrating ROOT with state-of-the-art distributed data processing technologies like Spark, both in terms of programming model and runtime design (with EOS as one of the main components). For all the levels of parallelism, we discuss, based on real-life examples and measurements, how our proposals can increase the productivity of scientists.

  9. Crescimento, morfologia radicular e liberação de compostos orgânicos por plântulas de soja em função da atividade de alumínio na solução do solo de campo natural Growth, root morphology and organic compounds released by soybean seedlings as a function of aluminum activity in a field soil solution

    Directory of Open Access Journals (Sweden)

    Antonio Nolla

    2007-02-01

    Full Text Available A toxicidade do alumínio em solos é considerada uma das maiores limitações para a produção das culturas. No entanto, algumas plantas são capazes de tolerar altas concentrações de alumínio por sua complexação com ácidos orgânicos exsudados pelas raízes. No intuito de estudar os efeitos concomitantes de faixas pH e de alumínio no desenvolvimento radicular de plântulas de soja, desenvolveu-se um trabalho na solução de um Argissolo Vermelho distrófico típico de campo natural. Cultivou-se, em câmara de crescimento, plântulas pré-germinadas de soja durante três dias dentro de tubos de ensaio aerados contendo 40ml da solução do solo (4,2µmol de Al L-1, submetidos a quatro concentrações de alumínio (0,0; 0,3; 0,6 e 1,2µmol L-1 e três níveis de pH (4,0; 5,0 e 6,0. O crescimento radicular e da parte aérea da soja foi menor em condições mais ácidas (pH 4,0. Em condições de acidez intermediária (pH H2O 5,0, o crescimento radicular foi menos afetado pelas espécies rizotóxicas de alumínio, mesmo quando submetidas a elevadas concentrações de alumínio na solução do solo.Aluminum toxicity in soils is considered one of the major limitations to crop production. However, some plants are able to tolerate high aluminum concentrations due to its complexation with root organic acids exudates. The simultaneous effect of pH ranges and aluminum concentration in soil solution on soybean seedlings root development was studied in a Rhodic Paleudult soil solution under natural grasses vegetation. Soybean seedlings were grown in growth chamber for three days in aerated test tubes containing 40ml soil solution (4.2µmol Al L-1, submmited to four aluminum concentrations (0.0, 0.3, 0.6 and 1.2mmol L-1 and three pH levels (4.0, 5.0 and 6.0. Root and aerial growth decreased under more acidic conditions (pH 4.0. Root growth was less affected by rhyzotoxic aluminum species at pH 5.0, even when submitted to high concentration of

  10. The PandaRoot framework for simulation, reconstruction and analysis

    International Nuclear Information System (INIS)

    Spataro, Stefano

    2011-01-01

    The PANDA experiment at the future facility FAIR will study anti-proton proton and anti-proton nucleus collisions in a beam momentum range from 2 GeV/c up to 15 GeV/c. The PandaRoot framework is part of the FairRoot project, a common software framework for the future FAIR experiments, and is currently used to simulate detector performances and to evaluate different detector concepts. It is based on the packages ROOT and Virtual MonteCarlo with Geant3 and Geant4. Different reconstruction algorithms for tracking and particle identification are under development and optimization, in order to achieve the performance requirements of the experiment. In the central tracker a first track fit is performed using a conformal map transformation based on a helix assumption, then the track is used as input for a Kalman Filter (package genfit), using GEANE as track follower. The track is then correlated to the pid detectors (e.g. Cerenkov detectors, EM Calorimeter or Muon Chambers) to evaluate a global particle identification probability, using a Bayesian approach or multivariate methods. Further implemented packages in PandaRoot are: the analysis tools framework Rho, the kinematic fitter package for vertex and mass constraint fits, and a fast simulation code based upon parametrized detector responses. PandaRoot was also tested on an Alien-based GRID infrastructure. The contribution will report about the status of PandaRoot and show some example results for analysis of physics benchmark channels.

  11. Isolamento e seleção de fungos causadores da podridão-branca da madeira em florestas de Eucalyptus spp. com potencial de degradação de cepas e raízes Isolation and screening of wood white rot fungi from Eucalyptus spp. forests with potential for use in degradation of stumps and roots

    Directory of Open Access Journals (Sweden)

    Sandra Kunieda de Alonso

    2007-02-01

    Full Text Available Este trabalho objetivou isolar fungos causadores da podridão-branca da madeira, a partir de basidiocarpos e de fragmentos de madeira de eucalipto coletados em várias regiões do país, bem como testar seu potencial de degradação de cepas e raízes mortas em plantios comerciais de eucalipto, após o corte raso. Para o isolamento dos fungos foi desenvolvido um meio de cultura de serragem de eucalipto-ágar. Dentre 292 isolados obtidos e submetidos ao teste de Bavendamm, 144 foram classificados como causadores de podridão-branca, capazes de produzir fenoloxidases. Dentre as nove relações C/N testadas, observou-se uma tendência de ocorrer maior degradação de cavacos naquelas iguais a 60 : 1, 200 : 1 e 300 : 1. Utilizando a relação C/N igual a 60 : 1, realizaram-se dois experimentos para avaliar a degradação de cavacos de Eucalyptus saligna por isolados fúngicos de podridão-branca. No primeiro experimento, avaliado aos 90 dias de incubação, foram selecionados sete isolados, que causaram perda de peso em cavacos superior ou igual à causada por Trametes versicolor, usado para comparação. No segundo experimento foram testados 46 isolados fúngicos. Dentre os mais eficientes estavam os sete isolados selecionados no primeiro teste, além de outros quatro isolados. Baseado na análise de DNA, seis isolados foram identificados, sendo três pertencentes à espécie Pycnoporus sanguineus, um ao gênero Peniophora sp., um ao gênero Pestalotiopsis sp. e um ao gênero Ganoderma sp.The aim of this work was to isolate native wood white-rot fungi from fungal fruit-bodies and eucalyptus wood fragments from different regions of Brazil and to test their potential for degrading dead stumps and roots in Eucalyptus plantings after harvest. Fungi isolates were obtained in a culture medium composed by Eucalyptus sawdust and agar. Among 292 isolates submitted to the Banvedamm test, 144 were classified as phenoloxidases producing isolates. Among nine C

  12. Root hairs increase root exudation and rhizosphere extension

    Science.gov (United States)

    Holz, Maire; Zarebandanadkouki, Mohsen; Kuzyakov, Yakov; Carmintati, Andrea

    2017-04-01

    Plant roots employ various mechanisms to increase their access to limited soil resources. An example of such strategies is the production of root hairs. Root hairs extend the root surface and therefore increase the access to nutrients. Additionally, carbon release from root hairs might facilitate nutrient uptake by spreading of carbon in the rhizosphere and enhancing microbial activity. The aim of this study was to test: i) how root hairs change the allocation of carbon in the soil-plant system; ii) whether root hairs exude carbon into the soil and iii) how differences in C release between plants with and without root hairs affect rhizosphere extension. We grew barley plants with and without root hairs (wild type: WT, bald root barley: brb) in rhizoboxes filled with a sandy soil. Root elongation was monitored over time. After 4 weeks of growth, plants were labelled with 14CO2. A filter paper was placed on the soil surface before labelling and was removed after 36 h. 14C imaging of the soil surface and of the filter paper was used to quantify the allocation of 14C into the roots and the exudation of 14C, respectively. Plants were sampled destructively one day after labeling to quantify 14C in the plant-soil system. 14CO2 release from soil over time (17 d) was quantified by trapping CO2 in NaOH with an additional subset of plants. WT and brb plants had a similar aboveground biomass and allocated similar amounts of 14C into shoots (170 KBq for WT; 152 KBq for brb) and roots one day after labelling. Biomass of root, rhizosphere soil as well as root elongation were lower for brb compared to the wild type. WT plants transported more C from the shoots to the roots (22.8% for WT; 13.8% for brb) and from the root into the rhizosphere (8.8% for WT 3.5% for brb). Yet lower amounts of 14CO2 were released from soil over time for WT. Radial and longitudinal rhizosphere extension was increased for WT compared to brb (4.7 vs. 2.6 mm; 5.6 vs. 3.1 cm). The total exudation which was

  13. Hydraulic conductivity of rice roots.

    Science.gov (United States)

    Miyamoto, N; Steudle, E; Hirasawa, T; Lafitte, R

    2001-09-01

    A pressure chamber and a root pressure probe technique have been used to measure hydraulic conductivities of rice roots (root Lp(r) per m(2) of root surface area). Young plants of two rice (Oryza sativa L.) varieties (an upland variety, cv. Azucena and a lowland variety, cv. IR64) were grown for 31-40 d in 12 h days with 500 micromol m(-2) s(-1) PAR and day/night temperatures of 27 degrees C and 22 degrees C. Root Lp(r) was measured under conditions of steady-state and transient water flow. Different growth conditions (hydroponic and aeroponic culture) did not cause visible differences in root anatomy in either variety. Values of root Lp(r) obtained from hydraulic (hydrostatic) and osmotic water flow were of the order of 10(-8) m s(-1) MPa(-1) and were similar when using the different techniques. In comparison with other herbaceous species, rice roots tended to have a higher hydraulic resistance of the roots per unit root surface area. The data suggest that the low overall hydraulic conductivity of rice roots is caused by the existence of apoplastic barriers in the outer root parts (exodermis and sclerenchymatous (fibre) tissue) and by a strongly developed endodermis rather than by the existence of aerenchyma. According to the composite transport model of the root, the ability to adapt to higher transpirational demands from the shoot should be limited for rice because there were minimal changes in root Lp(r) depending on whether hydrostatic or osmotic forces were acting. It is concluded that this may be one of the reasons why rice suffers from water shortage in the shoot even in flooded fields.

  14. [Effects of fertilization on fine root diameter, root length and specific root length in Larix kaempferi plantation].

    Science.gov (United States)

    Yu, Li-zhong; Ding, Guo-quan; Shi, Jian-wei; Yu, Shui-qiang; Zhu, Jiao-jun; Zhao, Lian-fu

    2007-05-01

    With 16 years old Larix kaempfersoil plantation in the mountainous area of eastern Liaoning Province as test object, this paper studied the effects of fertilization on the fine root diameter, root length, and specific root length (SRL) of the first to fifth order roots. The results showed that with ascending root orders, the mean fine root diameter and root length increased, while the SRL decreased significantly. Among the five order roots, the first order roots were the thinnest in diameter, the shortest in length, and the highest in SRL, but the fifth order roots were in reverse. The variance coefficients for the fine root diameter, root length, and SRL increased from the first to the fifth order roots. Except for the first order roots, soil depth had no significant influence on the fine root diameter, root length and SRL. Fertilization affected the fine root diameter, root length, and SRL of the first and the second order roots significantly, hut had little effects on other order roots. N fertilization decreased the mean diameter of the first and the second order roots significantly, and N or N + P fertilization decreased the mean length of the first order roots in surface soil (0-10 cm) significantly. The SRL of the first order roots in surface soil increased significantly under N fertilization.

  15. The Roots of Beowulf

    Science.gov (United States)

    Fischer, James R.

    2014-01-01

    The first Beowulf Linux commodity cluster was constructed at NASA's Goddard Space Flight Center in 1994 and its origins are a part of the folklore of high-end computing. In fact, the conditions within Goddard that brought the idea into being were shaped by rich historical roots, strategic pressures brought on by the ramp up of the Federal High-Performance Computing and Communications Program, growth of the open software movement, microprocessor performance trends, and the vision of key technologists. This multifaceted story is told here for the first time from the point of view of NASA project management.

  16. Matching roots to their environment.

    Science.gov (United States)

    White, Philip J; George, Timothy S; Gregory, Peter J; Bengough, A Glyn; Hallett, Paul D; McKenzie, Blair M

    2013-07-01

    Plants form the base of the terrestrial food chain and provide medicines, fuel, fibre and industrial materials to humans. Vascular land plants rely on their roots to acquire the water and mineral elements necessary for their survival in nature or their yield and nutritional quality in agriculture. Major biogeochemical fluxes of all elements occur through plant roots, and the roots of agricultural crops have a significant role to play in soil sustainability, carbon sequestration, reducing emissions of greenhouse gasses, and in preventing the eutrophication of water bodies associated with the application of mineral fertilizers. This article provides the context for a Special Issue of Annals of Botany on 'Matching Roots to Their Environment'. It first examines how land plants and their roots evolved, describes how the ecology of roots and their rhizospheres contributes to the acquisition of soil resources, and discusses the influence of plant roots on biogeochemical cycles. It then describes the role of roots in overcoming the constraints to crop production imposed by hostile or infertile soils, illustrates root phenotypes that improve the acquisition of mineral elements and water, and discusses high-throughput methods to screen for these traits in the laboratory, glasshouse and field. Finally, it considers whether knowledge of adaptations improving the acquisition of resources in natural environments can be used to develop root systems for sustainable agriculture in the future.

  17. RootJS : Node.js Bindings for ROOT 6

    OpenAIRE

    Beffart, Theo; Früh, Maximilian; Haas, Christoph; Rajgopal, Sachin; Schwabe, Jonas; Wolff, Christoph; Szuba, Marek

    2017-01-01

    We present rootJS, an interface making it possible to seamlessly integrate ROOT 6 into applications written for Node.js, the JavaScript runtime platform increasingly commonly used to create high-performance Web applications. ROOT features can be called both directly from Node.js code and by JIT-compiling C++ macros. All rootJS methods are invoked asynchronously and support callback functions, allowing non-blocking operation of Node.js applications using them. Last but not least, our bindings ...

  18. ROOT Tutorial for Summer Students

    CERN Multimedia

    CERN. Geneva; Piparo, Danilo

    2015-01-01

    ROOT is a "batteries-included" tool kit for data analysis, storage and visualization. It is widely used in High Energy Physics and other disciplines such as Biology, Finance and Astrophysics. This event is an introductory tutorial to ROOT and comprises a front lecture and hands on exercises. IMPORTANT NOTE: The tutorial is based on ROOT 6.04 and NOT on the ROOT5 series.  IMPORTANT NOTE: if you have ROOT 6.04 installed on your laptop, you will not need to install any virtual machine. The instructions showing how to install the virtual machine on which you can find ROOT 6.04 can be found under "Material" on this page.

  19. Removal of root filling materials.

    LENUS (Irish Health Repository)

    Duncan, H.F. Chong, B.S.

    2011-05-01

    Safe, successful and effective removal of root filling materials is an integral component of non-surgical root canal re-treatment. Access to the root canal system must be achieved in order to negotiate to the canal terminus so that deficiencies in the original treatment can be rectified. Since a range of materials have been advocated for filling root canals, different techniques are required for their removal. The management of commonly encountered root filling materials during non-surgical re-treatment, including the clinical procedures necessary for removal and the associated risks, are reviewed. As gutta-percha is the most widely used and accepted root filling material, there is a greater emphasis on its removal in this review.

  20. Comparing Leaf and Root Insertion

    Directory of Open Access Journals (Sweden)

    Jaco Geldenhuys

    2010-07-01

    Full Text Available We consider two ways of inserting a key into a binary search tree: leaf insertion which is the standard method, and root insertion which involves additional rotations. Although the respective cost of constructing leaf and root insertion binary search trees trees, in terms of comparisons, are the same in the average case, we show that in the worst case the construction of a root insertion binary search tree needs approximately 50% of the number of comparisons required by leaf insertion.

  1. Root communication among desert shrubs.

    OpenAIRE

    Mahall, B E; Callaway, R M

    1991-01-01

    Descriptive and experimental studies of desert shrub distributions have revealed important questions about the mechanisms by which plants interact. For example, do roots interact by mechanisms other than simple competition for limiting resources? We investigated this question using the desert shrubs Ambrosia dumosa and Larrea tridentata grown in chambers that allowed observation of roots during intraplant and intra- and interspecific interplant encounters. Two types of root "communication" we...

  2. Facilitative root interactions in intercrops

    DEFF Research Database (Denmark)

    Hauggaard-Nielsen, H.; Jensen, E.S.

    2005-01-01

    Facilitation takes place when plants ameliorate the environment of their neighbours, and increase their growth and survival. Facilitation occurs in natural ecosystems as well as in agroecosystems. We discuss examples of facilitative root interactions in intercropped agroecosystems; including...... of root architecture, exudation of growth stimulating substances, and biofumigation. Facilitative root interactions are most likely to be of importance in nutrient poor soils and in low-input agroecosystems due to critical interspecific competition for plant growth factors. However, studies from more...

  3. Proteomics of Maize Root Development.

    Science.gov (United States)

    Hochholdinger, Frank; Marcon, Caroline; Baldauf, Jutta A; Yu, Peng; Frey, Felix P

    2018-01-01

    Maize forms a complex root system with structurally and functionally diverse root types that are formed at different developmental stages to extract water and mineral nutrients from soil. In recent years proteomics has been intensively applied to identify proteins involved in shaping the three-dimensional architecture and regulating the function of the maize root system. With the help of developmental mutants, proteomic changes during the initiation and emergence of shoot-borne, lateral and seminal roots have been examined. Furthermore, root hairs were surveyed to understand the proteomic changes during the elongation of these single cell type structures. In addition, primary roots have been used to study developmental changes of the proteome but also to investigate the proteomes of distinct tissues such as the meristematic zone, the elongation zone as well as stele and cortex of the differentiation zone. Moreover, subcellular fractions of the primary root including cell walls, plasma membranes and secreted mucilage have been analyzed. Finally, the superior vigor of hybrid seedling roots compared to their parental inbred lines was studied on the proteome level. In summary, these studies provide novel insights into the complex proteomic interactions of the elaborate maize root system during development.

  4. Proteomics of Maize Root Development

    Directory of Open Access Journals (Sweden)

    Frank Hochholdinger

    2018-03-01

    Full Text Available Maize forms a complex root system with structurally and functionally diverse root types that are formed at different developmental stages to extract water and mineral nutrients from soil. In recent years proteomics has been intensively applied to identify proteins involved in shaping the three-dimensional architecture and regulating the function of the maize root system. With the help of developmental mutants, proteomic changes during the initiation and emergence of shoot-borne, lateral and seminal roots have been examined. Furthermore, root hairs were surveyed to understand the proteomic changes during the elongation of these single cell type structures. In addition, primary roots have been used to study developmental changes of the proteome but also to investigate the proteomes of distinct tissues such as the meristematic zone, the elongation zone as well as stele and cortex of the differentiation zone. Moreover, subcellular fractions of the primary root including cell walls, plasma membranes and secreted mucilage have been analyzed. Finally, the superior vigor of hybrid seedling roots compared to their parental inbred lines was studied on the proteome level. In summary, these studies provide novel insights into the complex proteomic interactions of the elaborate maize root system during development.

  5. Properties of Estimated Characteristic Roots

    DEFF Research Database (Denmark)

    Nielsen, Bent; Nielsen, Heino Bohn

    Estimated characteristic roots in stationary autoregressions are shown to give rather noisy information about their population equivalents. This is remarkable given the central role of the characteristic roots in the theory of autoregressive processes. In the asymptotic analysis the problems appear...... when multiple roots are present as this imply a non-differentiability so the d-method does not apply, convergence rates are slow, and the asymptotic distribution is non-normal. In finite samples this has a considerable influence on the finite sample distribution unless the roots are far apart...

  6. Produção e qualidade de sementes e raízes de cenoura cultivada em solo com adubação orgânica e mineral Production and quality of seeds and roots of carrot cultivated under organic and mineral fertilization

    Directory of Open Access Journals (Sweden)

    Riselane de Lucena Alcântara Bruno

    2007-06-01

    Full Text Available A ausência de sementes orgânicas no mercado e a escassez de informações sobre o assunto têm sido fortes empecilhos para a produção orgânica. Avaliou-se a eficiência da adubação verde (AV, do composto orgânico (CO e do biofertilizante (B na produção da cenoura e na qualidade das sementes, comparados à adubação convencional (AC-testemunha, nos tratamentos: 1 AC; 2 AV+B via solo; 3 AV+B via planta; 4 CO+B via solo; 5 CO+B via planta; 6 CO+AV+B via solo e 7 CO+AV+B via planta. Os maiores valores morfológicos (altura, comprimento e diâmetro, de germinação e de vigor foram proporcionados pelo tratamento 5, enquanto a produção e os sólidos solúveis totais alcançaram maiores gradientes nas plantas cultivadas com o tratamento 4, superando os dados do tratamento 1. O tratamento 3 proporcionou produção de raízes com menor número de galhas de nematóide. As cenouras procedentes dos tratamentos 2 e 6 foram classificadas em tamanho curto. A decomposição do AV, associado ao B via planta, pode ter proporcionado maior resistência das cenouras aos nematóides e melhor classificação comercial. Os tratamentos orgânicos proporcionaram boa qualidade de raízes, sementes e maior produção.The inexistence of organic seeds in the market and the absence of information constitute a difficulty for the organic production. The green fertilizer efficiency (GF was evaluated, of organic compound (OC, and biofertilizer (B in carrot production and seeds quality, compared to conventional fertilizer (CF-control, through the treatments: 1 CF; 2, GF+B via soil; 3 GF+B via plant; 4 OC+B via soil; 5 OC+B via plant; 6 OC+GF+B via soil and 7 OC+GF+B via plant. Higher morphological values (height, length and diameter, of germination and vigor were given by treatment 5, while production and total soluble solids reached higher gradients when growing plants with treatment 4, upper data of treatment 1. Treatment 3 resulted in root production with lower

  7. Root anatomy of nine orchidaceae species

    Directory of Open Access Journals (Sweden)

    Virginia del Carmem Oliveira

    1999-01-01

    Full Text Available Studies on the root anatomy of nine Orchidaceae species presented a multisseriated velamen, a parenchymatous cortex and a definided endodermis, in all the studied species (Catasetum fimbriatum Lindl., Dichaea bryophila Rchb. f., Encyclia calamara(Lindl. Pabst, Epidendrum campestre Lindl., Epidendrum secundum Jacq., Miltonia flavescens Lindl., Pleurothallis smithiana Lindl., Stanhopea lietzei (Regel Schltr. and Vanda tricolor Lindl. Structural characters, which could be considered root adaptations to an epiphytic habit, were also common for all species.As raízes possuem um velame multisseriado, um córtex parenquimático e uma endoderme bem definida, em todas as Orchidaceae estudadas (Catasetum fimbriatum Lindl., Dichaea bryophila Rchb. f., Encyclia calamara (Lindl.Pabst, Epidendrum campestre Lindl., Epidendrum secundum Jacq., Miltonia flavescens Lindl., Pleurothallis smithiana Lindl., Stanhopea lietzei (Regel Schltr.e Vanda tricolor Lindl.. Caracteres estruturais, que podem ser considerados adaptações ao hábito epífito, são comuns nas raízes estudadas.

  8. Sesquiterpene lactones and inulin from chicory roots : extraction, identification, enzymatic release and sensory analysis

    OpenAIRE

    Leclercq, E.

    1992-01-01

    Chicory ( <em>Cichorium intybusem> L.) is one of the many species of the family Compositae. Chicory has been cultivated for the production of leaves or chicons, which have been used as a vegetable since approximately 300 BC, and for its roots, which can be used as a coffee substitute after roasting.

    Chicory leaves are appreciated for their slightly bitter taste. Two bitter compounds were known at the start of this project: lactucin...

  9. Effects of different concentarions of auxins on rooting and root ...

    African Journals Online (AJOL)

    The effect of auxins and their different concentrations on rooting and root characters of air and ground layers of jojoba was assessed at Maxima Estate Private Limited Farm, Hyderabad, India in 1998. Auxins IBA, NAA and their mixture (IBA + NAA) at concentrations of 1000, 2000, 4000 and 6000 ppm with lanolin paste were ...

  10. Radiographing roots and shoots

    International Nuclear Information System (INIS)

    Shariffah Noor Khamseah Al Idid

    1985-01-01

    The effect of seed orientation on germination time and on shoot and root growth patterns is studied. Neutron radiography is used to observe the development of 4 types of plants, maize, greenpea, soya bean and padi. These plants were grown in varying orientations; sand sizes, sand thicknesses, and level of water content. Radiography of the seeds and plants were obtained for time exposure ranging from 3-12 hours and at reactor thermal power level, ranging from 500-750 kilowatts. Results obtained showed that seeds planted in varying orientations need different length of time for shoot emergence. Neutron radiography is now developed to other areas of non-industrial applications in Malaysia. (A.J.)

  11. Back to the roots!

    DEFF Research Database (Denmark)

    Woermann, Niklas

    2017-01-01

    This article argues that one can revive the critical edge that postmodernist theory has brought to marketing, thinking without subscribing to any particular school of (critical) theory by following the principle of methodological situationalism. The roots of postmodernist critique lie in careful...... empirical observation of how social reality is being constructed in local contexts. Because knowledge, subjects, power, and value are social accomplishments, they are neither fixed nor without alternative. Many key developments in marketing theory such as assemblage theory, practice and consumer tribes...... of social order into account, hence fail to provide sensible insight. I propose the principle of methodological situationalism as a litmus test to the analytical strength of a theory or piece of research. The principle states that theoretically adequate accounts of social phenomena must be grounded...

  12. Reference Gene Selection in the Desert Plant <em>Eremosparton songoricuem>m>

    Directory of Open Access Journals (Sweden)

    Dao-Yuan Zhang

    2012-06-01

    Full Text Available <em>Eremosparton songoricum em>(Litv. Vass. (<em>E. songoricumem> is a rare and extremely drought-tolerant desert plant that holds promise as a model organism for the identification of genes associated with water deficit stress. Here, we cloned and evaluated the expression of eight candidate reference genes using quantitative real-time reverse transcriptase polymerase chain reactions. The expression of these candidate reference genes was analyzed in a diverse set of 20 samples including various <em>E. songoricumem> plant tissues exposed to multiple environmental stresses. GeNorm analysis indicated that expression stability varied between the reference genes in the different experimental conditions, but the two most stable reference genes were sufficient for normalization in most conditions.<em> EsEFem> and <em>Esα-TUB> were sufficient for various stress conditions, <em>EsEF> and <em>EsACT> were suitable for samples of differing germination stages, and <em>EsGAPDH>and <em>Es>UBQ em>were most stable across multiple adult tissue samples. The <em>Es18Sem> gene was unsuitable as a reference gene in our analysis. In addition, the expression level of the drought-stress related transcription factor <em>EsDREB2em>> em>verified the utility of<em> E. songoricumem> reference genes and indicated that no single gene was adequate for normalization on its own. This is the first systematic report on the selection of reference genes in <em>E. songoricumem>, and these data will facilitate future work on gene expression in this species.

  13. Searching for Roots / Pierre Gervasoni

    Index Scriptorium Estoniae

    Gervasoni, Pierre

    1997-01-01

    Uuest heliplaadist "Searching for Roots. Eduard Tubin: Symphonie no 11; Arvo Pärt: Nekrolog-Symphonie no 1; Erkki-Sven Tüür: Searching for Roots - Insula deserta - Zeitraum; Orchestre philharmonique royal de Stockholm, Paavo Järvi (direction)" Virgin Classics 5 45212 2 (distribue par EMI)

  14. Cytokinin signaling during root development.

    Science.gov (United States)

    Bishopp, Anthony; Help, Hanna; Helariutta, Ykä

    2009-01-01

    The cytokinin class of phytohormones regulates division and differentiation of plant cells. They are perceived and signaled by a phosphorelay mechanism similar to those observed in prokaryotes. Research into the components of phosphorelay had previously been marred by genetic redundancy. However, recent studies have addressed this with the creation of high-order mutants. In addition, several new elements regulating cytokinin signaling have been identified. This has uncovered many roles in diverse developmental and physiological processes. In this review, we look at these processes specifically in the context of root development. We focus on the formation and maintenance of the root apical meristem, primary and secondary vascular development, lateral root emergence and development, and root nodulation. We believe that the root is an ideal organ with which to investigate cytokinin signaling in a wider context.

  15. Physical root-soil interactions.

    Science.gov (United States)

    Kolb, Evelyne; Legué, Valérie; Bogeat-Triboulot, Marie-Béatrice

    2017-11-16

    Plant root system development is highly modulated by the physical properties of the soil and especially by its mechanical resistance to penetration. The interplay between the mechanical stresses exerted by the soil and root growth is of particular interest for many communities, in agronomy and soil science as well as in biomechanics and plant morphogenesis. In contrast to aerial organs, roots apices must exert a growth pressure to penetrate strong soils and reorient their growth trajectory to cope with obstacles like stones or hardpans or to follow the tortuous paths of the soil porosity. In this review, we present the main macroscopic investigations of soil-root physical interactions in the field and combine them with simple mechanistic modeling derived from model experiments at the scale of the individual root apex.

  16. Roots of the Chromatic Polynomial

    DEFF Research Database (Denmark)

    Perrett, Thomas

    The chromatic polynomial of a graph G is a univariate polynomial whose evaluation at any positive integer q enumerates the proper q-colourings of G. It was introduced in connection with the famous four colour theorem but has recently found other applications in the field of statistical physics....... In this thesis we study the real roots of the chromatic polynomial, termed chromatic roots, and focus on how certain properties of a graph affect the location of its chromatic roots. Firstly, we investigate how the presence of a certain spanning tree in a graph affects its chromatic roots. In particular we prove...... of certain minor-closed families of graphs. Later, we study the Tutte polynomial of a graph, which contains the chromatic polynomial as a specialisation. We discuss a technique of Thomassen using which it is possible to deduce that the roots of the chromatic polynomial are dense in certain intervals. We...

  17. Descendant root volume varies as a function of root type: estimation of root biomass lost during uprooting in Pinus pinaster.

    Science.gov (United States)

    Danjon, Frédéric; Caplan, Joshua S; Fortin, Mathieu; Meredieu, Céline

    2013-01-01

    Root systems of woody plants generally display a strong relationship between the cross-sectional area or cross-sectional diameter (CSD) of a root and the dry weight of biomass (DWd) or root volume (Vd) that has grown (i.e., is descendent) from a point. Specification of this relationship allows one to quantify root architectural patterns and estimate the amount of material lost when root systems are extracted from the soil. However, specifications of this relationship generally do not account for the fact that root systems are comprised of multiple types of roots. We assessed whether the relationship between CSD and Vd varies as a function of root type. Additionally, we sought to identify a more accurate and time-efficient method for estimating missing root volume than is currently available. We used a database that described the 3D root architecture of Pinus pinaster root systems (5, 12, or 19 years) from a stand in southwest France. We determined the relationship between CSD and Vd for 10,000 root segments from intact root branches. Models were specified that did and did not account for root type. The relationships were then applied to the diameters of 11,000 broken root ends to estimate the volume of missing roots. CSD was nearly linearly related to the square root of Vd, but the slope of the curve varied greatly as a function of root type. Sinkers and deep roots tapered rapidly, as they were limited by available soil depth. Distal shallow roots tapered gradually, as they were less limited spatially. We estimated that younger trees lost an average of 17% of root volume when excavated, while older trees lost 4%. Missing volumes were smallest in the central parts of root systems and largest in distal shallow roots. The slopes of the curves for each root type are synthetic parameters that account for differentiation due to genetics, soil properties, or mechanical stimuli. Accounting for this differentiation is critical to estimating root loss accurately.

  18. RootJS: Node.js Bindings for ROOT 6

    Science.gov (United States)

    Beffart, Theo; Früh, Maximilian; Haas, Christoph; Rajgopal, Sachin; Schwabe, Jonas; Wolff, Christoph; Szuba, Marek

    2017-10-01

    We present rootJS, an interface making it possible to seamlessly integrate ROOT 6 into applications written for Node.js, the JavaScript runtime platform increasingly commonly used to create high-performance Web applications. ROOT features can be called both directly from Node.js code and by JIT-compiling C++ macros. All rootJS methods are invoked asynchronously and support callback functions, allowing non-blocking operation of Node.js applications using them. Last but not least, our bindings have been designed to platform-independent and should therefore work on all systems supporting both ROOT 6 and Node.js. Thanks to rootJS it is now possible to create ROOT-aware Web applications taking full advantage of the high performance and extensive capabilities of Node.js. Examples include platforms for the quality assurance of acquired, reconstructed or simulated data, book-keeping and e-log systems, and even Web browser-based data visualisation and analysis.

  19. Medico-legal aspects of vertical root fractures in root filled teeth

    DEFF Research Database (Denmark)

    Rosen, E; Tsesis, I; Tamse, A

    2012-01-01

    To analyse the medico-legal aspects of vertical root fracture (VRF) following root canal treatment (RCT).......To analyse the medico-legal aspects of vertical root fracture (VRF) following root canal treatment (RCT)....

  20. Root anatomical phenes predict root penetration ability and biomechanical properties in maize (Zea Mays)

    OpenAIRE

    Chimungu, Joseph G.; Loades, Kenneth W.; Lynch, Jonathan P.

    2015-01-01

    The ability of roots to penetrate hard soil is important for crop productivity but specific root phenes contributing to this ability are poorly understood. Root penetrability and biomechanical properties are likely to vary in the root system dependent on anatomical structure. No information is available to date on the influence of root anatomical phenes on root penetrability and biomechanics. Root penetration ability was evaluated using a wax layer system. Root tensile and bending strength we...

  1. Intervalo hídrico ótimo no perfil explorado pelas raízes de feijoeiro em um latossolo sob diferentes níveis de compactação Least limiting water range in an oxisol profile penetrated by common bean roots under different compaction levels

    Directory of Open Access Journals (Sweden)

    Douglas Rodrigo Kaiser

    2009-08-01

    Full Text Available O intervalo hídrico ótimo (IHO é integrador dos fatores de crescimento das plantas, e a densidade crítica obtida é um indicativo da qualidade estrutural do solo. O objetivo deste trabalho foi determinar o IHO em um Latossolo argiloso. Amostras de solo com estrutura preservada foram coletadas num experimento com três níveis de compactação: PD - plantio direto continuado por seis anos, PDc - plantio direto com compactação adicional e Esc - escarificação. Para a curva de resistência, coletaram-se 107 amostras na camada de 0 a 0,20 m em diferentes condições de umidade. Para a curva de retenção de água, coletaram-se amostras nas camadas de 0 a 0,05; 0,05 a 0,10; 0,10 a 0,15; 0,20 a 0,25; e 0,30 a 0,35 m. O modelo de resistência à penetração ajustado, com base na densidade e umidade, explicou 33 % da variação obtida na resistência do solo à penetração, sendo todos os parâmetros de ajuste significativos. A densidade crítica do IHO é dependente do valor de resistência à penetração considerado limitante, sendo de 1,36; 1,40; 1,45; e 1,49 Mg m-3 para valores de RP de 1,5; 2,0; 2,5; e 3,0 MPa, respectivamente. A aeração do solo passa a ser limitante com densidades acima de 1,32 Mg m-3. A compactação do solo reduziu o seu IHO na camada próxima a 0,10 m de profundidade. Quando se adotou a resistência crítica de 2 MPa, o IHO foi nulo nas camadas de 0,05 a 0,12 m no PD, de 0,05 a 0,17 m no Esc e de 0,03 a 0,22 m no PDc. Com a utilização de 3 MPa como resistência crítica, ocorreu ampliação, em que o IHO tem valor positivo, no perfil do solo; o IHO foi nulo apenas na camada de 0,05 a 0,15 m do PDc. As raízes do feijoeiro não cresceram na camada de solo onde o IHO foi nulo com resistência crítica de 3 MPa.The least limiting water range (LLWR integrates the plant growth factors and the critical bulk density is an indicator of the soil structural quality. The objective of this study was to determine the LLWR of a

  2. Root development during soil genesis: effects of root-root interactions, mycorrhizae, and substrate

    Science.gov (United States)

    Salinas, A.; Zaharescu, D. G.

    2015-12-01

    A major driver of soil formation is the colonization and transformation of rock by plants and associated microbiota. In turn, substrate chemical composition can also influence the capacity for plant colonization and development. In order to better define these relationships, a mesocosm study was set up to analyze the effect mycorrhizal fungi, plant density and rock have on root development, and to determine the effect of root morphology on weathering and soil formation. We hypothesized that plant-plant and plant-fungi interactions have a stronger influence on root architecture and rock weathering than the substrate composition alone. Buffalo grass (Bouteloua dactyloides) was grown in a controlled environment in columns filled with either granular granite, schist, rhyolite or basalt. Each substrate was given two different treatments, including grass-microbes and grass-microbes-mycorrhizae and incubated for 120, 240, and 480 days. Columns were then extracted and analyzed for root morphology, fine fraction, and pore water major element content. Preliminary results showed that plants produced more biomass in rhyolite, followed by schist, basalt, and granite, indicating that substrate composition is an important driver of root development. In support of our hypothesis, mycorrhizae was a strong driver of root development by stimulating length growth, biomass production, and branching. However, average root length and branching also appeared to decrease in response to high plant density, though this trend was only present among roots with mycorrhizal fungi. Interestingly, fine fraction production was negatively correlated with average root thickness and volume. There is also slight evidence indicating that fine fraction production is more related to substrate composition than root morphology, though this data needs to be further analyzed. Our hope is that the results of this study can one day be applied to agricultural research in order to promote the production of crops

  3. Non-canonical WOX11-mediated root branching contributes to plasticity in arabidopsis root system architecture

    NARCIS (Netherlands)

    Sheng, Lihong; Hu, Xiaomei; Du, Yujuan; Zhang, Guifang; Huang, Hai; Scheres, Ben; Xu, Lin

    2017-01-01

    Lateral roots (LRs), which originate from the growing root, and adventitious roots (ARs), which are formed from non-root organs, are the main contributors to the post-embryonic root system in Arabidopsis. However, our knowledge of how formation of the root system is altered in response to diverse

  4. Synthesis, Crystal Structure and Luminescent Property of Cd (II Complex with <em>N-Benzenesulphonyl-L>-leucine

    Directory of Open Access Journals (Sweden)

    Xishi Tai

    2012-09-01

    Full Text Available A new trinuclear Cd (II complex [Cd3(L6(2,2-bipyridine3] [L =<em> Nem>-phenylsulfonyl-L>-leucinato] has been synthesized and characterized by elemental analysis, IR and X-ray single crystal diffraction analysis. The results show that the complex belongs to the orthorhombic, space group<em> Pem>212121 with<em> aem> = 16.877(3 Å, <em>b> em>= 22.875(5 Å, <em>c em>= 29.495(6 Å, <em>α> em>= <emem>= <emem>= 90°, <em>V> em>= 11387(4 Å3, <em>Z> em>= 4, <em>Dc>= 1.416 μg·m−3, <emem>= 0.737 mm−1, <em>F> em>(000 = 4992, and final <em>R>1 = 0.0390, <em>ωR>2 = 0.0989. The complex comprises two seven-coordinated Cd (II atoms, with a N2O5 distorted pengonal bipyramidal coordination environment and a six-coordinated Cd (II atom, with a N2O4 distorted octahedral coordination environment. The molecules form one dimensional chain structure by the interaction of bridged carboxylato groups, hydrogen bonds and p-p interaction of 2,2-bipyridine. The luminescent properties of the Cd (II complex and <em>N-Benzenesulphonyl-L>-leucine in solid and in CH3OH solution also have been investigated.

  5. Hypocotyl adventitious root organogenesis differs from lateral root development.

    Science.gov (United States)

    Verstraeten, Inge; Schotte, Sébastien; Geelen, Danny

    2014-01-01

    Wound-induced adventitious root (AR) formation is a requirement for plant survival upon root damage inflicted by pathogen attack, but also during the regeneration of plant stem cuttings for clonal propagation of elite plant varieties. Yet, adventitious rooting also takes place without wounding. This happens for example in etiolated Arabidopsis thaliana hypocotyls, in which AR initiate upon de-etiolation or in tomato seedlings, in which AR initiate upon flooding or high water availability. In the hypocotyl AR originate from a cell layer reminiscent to the pericycle in the primary root (PR) and the initiated AR share histological and developmental characteristics with lateral roots (LRs). In contrast to the PR however, the hypocotyl is a determinate structure with an established final number of cells. This points to differences between the induction of hypocotyl AR and LR on the PR, as the latter grows indeterminately. The induction of AR on the hypocotyl takes place in environmental conditions that differ from those that control LR formation. Hence, AR formation depends on differentially regulated gene products. Similarly to AR induction in stem cuttings, the capacity to induce hypocotyl AR is genotype-dependent and the plant growth regulator auxin is a key regulator controlling the rooting response. The hormones cytokinins, ethylene, jasmonic acid, and strigolactones in general reduce the root-inducing capacity. The involvement of this many regulators indicates that a tight control and fine-tuning of the initiation and emergence of AR exists. Recently, several genetic factors, specific to hypocotyl adventitious rooting in A. thaliana, have been uncovered. These factors reveal a dedicated signaling network that drives AR formation in the Arabidopsis hypocotyl. Here we provide an overview of the environmental and genetic factors controlling hypocotyl-born AR and we summarize how AR formation and the regulating factors of this organogenesis are distinct from LR

  6. Hypocotyl adventitious root organogenesis differs from lateral root development

    Directory of Open Access Journals (Sweden)

    Inge eVerstraeten

    2014-09-01

    Full Text Available Wound-induced adventitious root (AR formation is a requirement for plant survival upon root damage inflicted by pathogen attack, but also during the regeneration of plant stem cuttings for clonal propagation of elite plant varieties. Yet, adventitious rooting also takes place without wounding. This happens for example in etiolated Arabidopsis thaliana hypocotyls, in which AR initiate upon de-etiolation or in tomato seedlings, in which AR initiate upon flooding or high water availability. In the hypocotyl AR originate from a cell layer reminiscent to the pericycle in the primary root (PR and the initiated AR share histological and developmental characteristics with lateral roots (LR. In contrast to the PR however, the hypocotyl is a determinate structure with an established final number of cells. This points to differences between the induction of hypocotyl AR and LR on the PR, as the latter grows indeterminately. The induction of AR on the hypocotyl takes place in environmental conditions that differ from those that control LR formation. Hence, AR formation depends on differentially regulated gene products. Similarly to AR induction in stem cuttings, the capacity to induce hypocotyl AR is genotype-dependent and the plant growth regulator auxin is a key regulator controlling the rooting response. The hormones cytokinins, ethylene, jasmonic acid and strigolactones in general reduce the root-inducing capacity. The involvement of this many regulators indicates that a tight control and fine-tuning of the initiation and emergence of AR exists. Recently, several genetic factors, specific to hypocotyl adventitious rooting in Arabidopsis thaliana, have been uncovered. These factors reveal a dedicated signaling network that drives AR formation in the Arabidopsis hypocotyl. Here we provide an overview of the environmental and genetic factors controlling hypocotyl-born AR and we summarize how AR formation and the regulating factors of this organogenesis are

  7. In vitro production of thiophenes using hairy root cultures of Tagetes ...

    African Journals Online (AJOL)

    Aghomotsegin

    2016-04-27

    Apr 27, 2016 ... Thiophene accumulation in relation to morphology in roots of. Tagetes patula effets of auxin and transformation by Agrobacterium. Planta 179: 43-50. Downum KR, Towers GHN (1983). Analysis of thiophenes in the. Tageteae (Asteraceae) by HPLC. J. Nat. Prod. 46: 98-103. Gareau BJ, Du Puis EM (2009).

  8. IAA transport in corn roots includes the root cap

    International Nuclear Information System (INIS)

    Hasenstein, K.H.

    1989-01-01

    In earlier reports we concluded that auxin is the growth regulator that controls gravicurvature in roots and that the redistribution of auxin occurs within the root cap. Since other reports did not detect auxin in the root cap, we attempted to confirm the IAA does move through the cap. Agar blocks containing 3 H-IAA were applied to the cut surface of 5 mm long apical segments of primary roots of corn (mo17xB73). After 30 to 120 min radioactivity (RA) of the cap and root tissue was determined. While segments suspended in water-saturated air accumulated very little RA in the cap, application of 0.5 μ1 of dist. water to the cap (=controls) increased RA of the cap dramatically. Application to the cap of 0.5 μ1 of sorbitol or the Ca 2+ chelator EGTA reduced cap RA to 46% and 70% respectively compared to water, without affecting uptake. Control root segments gravireacted faster than non-treated or osmoticum or EGTA treated segments. The data indicate that both the degree of hydration and calcium control the amount of auxin moving through the cap

  9. Root coverage with bridge flap

    Directory of Open Access Journals (Sweden)

    Pushpendra Kumar Verma

    2013-01-01

    Full Text Available Gingival recession in anterior teeth is a common concern due to esthetic reasons or root sensitivity. Gingival recession, especially in multiple anterior teeth, is of huge concern due to esthetic reasons. Various mucogingival surgeries are available for root coverage. This case report presents a new bridge flap technique, which allows the dentist not only to cover the previously denuded root surfaces but also to increase the zone of attached gingiva at a single step. In this case, a coronally advanced flap along with vestibular deepening technique was used as root coverage procedure for the treatment of multiple recession-type defect. Here, vestibular deepening technique is used to increase the width of the attached gingiva. The predictability of this procedure results in an esthetically healthy periodontium, along with gain in keratinized tissue and good patient′s acceptance.

  10. ISLSCP II Ecosystem Rooting Depths

    Data.gov (United States)

    National Aeronautics and Space Administration — The goal of this study was to predict the global distribution of plant rooting depths based on data about global aboveground vegetation structure and climate....

  11. ISLSCP II Ecosystem Rooting Depths

    Data.gov (United States)

    National Aeronautics and Space Administration — ABSTRACT: The goal of this study was to predict the global distribution of plant rooting depths based on data about global aboveground vegetation structure and...

  12. A Rooted Net of Life

    OpenAIRE

    Williams, David; Fournier, Gregory P.; Lapierre, Pascal; Swithers, Kristen S.; Green, Anna G.; Andam, Cheryl P.; Gogarten, J. Peter

    2011-01-01

    Abstract Phylogenetic reconstruction using DNA and protein sequences has allowed the reconstruction of evolutionary histories encompassing all life. We present and discuss a means to incorporate much of this rich narrative into a single model that acknowledges the discrete evolutionary units that constitute the organism. Briefly, this Rooted Net of Life genome phylogeny is constructed around an initial, well resolved and rooted tree scaffold inferred from a supermatrix of combined ribosomal g...

  13. Molecular Cloning and Functional Analysis of Three <em>FLOWERING LOCUS T (FTem> Homologous Genes from Chinese <em>Cymbidium>

    Directory of Open Access Journals (Sweden)

    Jaime A. Teixeira da Silva

    2012-09-01

    Full Text Available The <em>FLOWERING LOCUS Tem> (<em>FT> gene plays crucial roles in regulating the transition from the vegetative to reproductive phase. To understand the molecular mechanism of reproduction, three homologous <em>FT> genes were isolated and characterized from <em>Cymbidium sinenseem> “Qi Jian Bai Mo”, <em>Cymbidium goeringiiem> and <em>Cymbidium ensifoliumem> “Jin Si Ma Wei”. The three genes<em> em>contained 618-bp nucleotides with a 531-bp open reading frame (ORF of encoding 176 amino acids (AAs. Alignment of the AA sequences revealed that CsFT, CgFT and CeFT contain a conserved domain, which is characteristic of the<em> em>PEBP-RKIP superfamily, and which share high identity with FT of other plants in GenBank: 94% with OnFT<em> em>from <em>Oncidium em>Gower Ramsey, 79% with Hd3a from <em>Oryza sativaem>, and 74% with FT from <em>Arabidopsis thalianaem>. qRT-PCR analysis showed a diurnal expression pattern of <em>CsFT>, <em>CgFT> and <em>CeFT> following both long day (LD, 16-h light/8-h dark and short day (SD, 8-h light/16-h dark treatment. While the transcripts of both <em>CsFT em>and <em>CeFT em>under LD were significantly higher than under SD, those of <em>CgFT em>were> em>higher under SD. Ectopic expression of <em>CgFT> in transgenic <em>Arabidopsis> plants resulted in early flowering compared to wild-type plants and significant up-regulation of <em>APETALA1em> (<em>AP1em> expression. Our data indicates that CgFT is a putative phosphatidylethanolamine-binding protein gene in <em>Cymbidium> that may regulate the vegetative to reproductive transition in flowers, similar to its <em>Arabidopsis> ortholog.

  14. Avaliação de técnica cirúrgica parendodôntica: apicectomia em 90º, retrocavitação com ultra-som e retrobturação com MTA = Evaluation of endodontics surgical technique: apical resection with a 90 o angle, ultrasonic preparation of root-end cavities and trioxide aggregate (MTA) filling

    OpenAIRE

    Pozza, Daniel Humberto

    2005-01-01

    Técnicas consagradas como apicectomia por broca em 45 graus, retrocavidade com broca de aço esférica e retrobturação com amálgama tem sido gradativamente substituídas por novas seqüências de procedimentos que aumentam os benefícios cirúrgico, melhorando o prognóstico clínico. A metodologia utilizada neste artigo encontra-se ambasada em revisão bibliográfica e aplicação laboratorial. Isto possibilitou identificar inúmeras vantagens no uso da apicectomia em 90 graus, retrocavidade com ultra-som...

  15. Root Sparse Bayesian Learning for Off-Grid DOA Estimation

    Science.gov (United States)

    Dai, Jisheng; Bao, Xu; Xu, Weichao; Chang, Chunqi

    2017-01-01

    The performance of the existing sparse Bayesian learning (SBL) methods for off-gird DOA estimation is dependent on the trade off between the accuracy and the computational workload. To speed up the off-grid SBL method while remain a reasonable accuracy, this letter describes a computationally efficient root SBL method for off-grid DOA estimation, where a coarse refinable grid, whose sampled locations are viewed as the adjustable parameters, is adopted. We utilize an expectation-maximization (EM) algorithm to iteratively refine this coarse grid, and illustrate that each updated grid point can be simply achieved by the root of a certain polynomial. Simulation results demonstrate that the computational complexity is significantly reduced and the modeling error can be almost eliminated.

  16. <em>α>-Glucosidase Inhibitory Constituents from <em>Acanthopanax senticosusem> Harm Leaves

    Directory of Open Access Journals (Sweden)

    Hai-Xue Kuang

    2012-05-01

    Full Text Available A new triterpene glycoside, 3-<em>O-[(α>-L-rhamnopyranosyl(1→2]-[<em>β>-D-glucuronopyranosyl-6-<em>O>-methyl ester]-olean-12-ene-28-olic acid (1 and a new indole alkaloid, 5-methoxy-2-oxoindolin-3-acetic acid methyl ester (5 were isolated from the leaves of <em>Acanthopanax senticosusem> Harms along with six known compounds. The structures of the new compounds were determined by means of 2D-NMR experiments and chemical methods. All the isolated compounds were evaluated for their glycosidase inhibition activities and compound 6 showed significant <em>α>-glucosidase inhibition activity.

  17. Principles of crop modelling and simulation: III. modeling of root growth and other belowground processes, limitations of the models, and the future of modeling in agriculture Princípios de modelagem e simulação: III. modelagem do sistema radicular e outros processos subterrâneos, limitações dos modelos e o futuro da modelagem em agricultura

    Directory of Open Access Journals (Sweden)

    D. DOURADO-NETO

    1998-01-01

    Full Text Available The first models of temporal variation of root systems appeared over 20 years ago. The complex architectural geometry of root systems; the wide range in size and diameter and the rapid growth and decomposition of finest roots; the different physiological activity of roots of different ages; the complex microbial processes occurring at the root-soil interface; the symbiotic relationships in the rhizosphere; the variable soil environment (physical, chemical and biological in which roots develop are the challenges of quantifying the root growth. The models are not simple mechanisms to archive information in order to produce forecasts. Modeling represents a better way of synthesizing knowledge about different components of a system, summarizing data, and transferring research results to users.Os primeiros modelos que contemplaram a variação espacial e temporal de sistemas radiculares foram apresentados há mais de 20 anos. A complexa arquitetura dos sistemas radiculares; a ampla faixa de variação no tamanho e no diâmetro, bem como no rápido crescimento e decomposição de raízes finas; as diferentes atividades fisiológicas das raízes de diferentes idades; os complexos processos microbiológicos que ocorrem na interface raiz-solo; as relações simbióticas na rizosfera; as variações químicas, físicas e biológicas que ocorrem no solo (local onde as raízes se desenvolvem são os desafios da quantificação do crescimento radicular. Os modelos não são simples mecanismos para arquivar informações, produzindo estimativas. Os modelos representam o melhor mecanismo de sintetizar conhecimento sobre diferentes componentes do sistema, arquivando dados de forma sintética, e transferindo resultados de pesquisa para os usuários.

  18. Plant root-microbe communication in shaping root microbiomes.

    Science.gov (United States)

    Lareen, Andrew; Burton, Frances; Schäfer, Patrick

    2016-04-01

    A growing body of research is highlighting the impacts root-associated microbial communities can have on plant health and development. These impacts can include changes in yield quantity and quality, timing of key developmental stages and tolerance of biotic and abiotic stresses. With such a range of effects it is clear that understanding the factors that contribute to a plant-beneficial root microbiome may prove advantageous. Increasing demands for food by a growing human population increases the importance and urgency of understanding how microbiomes may be exploited to increase crop yields and reduce losses caused by disease. In addition, climate change effects may require novel approaches to overcoming abiotic stresses such as drought and salinity as well as new emerging diseases. This review discusses current knowledge on the formation and maintenance of root-associated microbial communities and plant-microbe interactions with a particular emphasis on the effect of microbe-microbe interactions on the shape of microbial communities at the root surface. Further, we discuss the potential for root microbiome modification to benefit agriculture and food production.

  19. A new method to optimize root order classification based on the diameter interval of fine root

    OpenAIRE

    Liu, Ying; Wang, Guoliang; Yu, Kunxia; Li, Peng; Xiao, Lie; Liu, Guobin

    2018-01-01

    Plant roots are a highly heterogeneous and hierarchical system. Although the root-order method is superior to the root diameter method for revealing differences in the morphology and physiology of fine roots, its complex partitioning limits its application. Whether root order can be determined by partitioning the main root based on its diameter remains uncertain. Four methods were employed for studying the morphological characteristics of seedling roots of two Pinus species in a natural and n...

  20. Root canal treatment of three-rooted mandibular second premolar using cone-beam computed tomography

    OpenAIRE

    Mohammad Ahmad Alenezi; Mohammad Abdullah Tarish; Deena J Alenezi

    2015-01-01

    A careful knowledge of root canals anatomy of different teeth is a corner stone for a successful outcome of root canal therapy. This reported case illustrates root canal therapy of a mandibular second premolar with three separated roots and root canals. An 18-year-old Saudi male presented for non-surgical endodontic treatment of mandibular right second premolar. Radiographic and clinical examinations revealed the presence of three roots and three root canals. The case was successfully managed...

  1. Tricolemoma em coelho Tricholemmoma in a rabbit

    Directory of Open Access Journals (Sweden)

    Krishna Duro de Oliveira

    1999-06-01

    Full Text Available Tricolemoma é um raro tumor benigno, composto por células epiteliais claras derivadas da bainha externa da raiz do folículo piloso. Esses tumores têm sido descritos no homem e raramente em cães. No presente relato, descreve-se a ocorrência de um tricolemoma, de 1cm de diâmetro em um coelho adulto, cujas características histológicas são muito semelhantes às descritas para esses tumores em cães.Tricholemmoma is a rare benign tumor composed of clear epithelial cells derived from the outer root sheath of the hair follicle. The tumor has been described in man and rarely in dogs. This report deals with the occurrence of a tricholemmoma , with a diemeter of 1cm, in an adult rabbit. The histologic features are similar to those described in such tumors in dogs.

  2. Descendant root volume varies as a function of root type: estimation of root biomass lost during uprooting in Pinus pinaster

    OpenAIRE

    Danjon, Frédéric; Caplan, Joshua S.; Fortin, Mathieu; Meredieu, Céline

    2013-01-01

    Root systems of woody plants generally display a strong relationship between the cross-sectional area or cross-sectional diameter (CSD) of a root and the dry weight of biomass (DWd) or root volume (Vd) that has grown (i.e., is descendent) from a point. Specification of this relationship allows one to quantify root architectural patterns and estimate the amount of material lost when root systems are extracted from the soil. However, specifications of this relationship generally do not account ...

  3. Avaliação de Trichoderma viride e formaldeído no controle da armilariose em plantios jovens de Pinus elliottii var. elliottii / Evaluation of Trichoderma viride and formaldehyde for control of Armillaria root rot in young plantings of Pinus elliottii var. elliottii

    Directory of Open Access Journals (Sweden)

    Celso Garcia Auer

    2012-08-01

    Full Text Available ResumoA armilariose é a principal doença em plantios comerciais de pínus na região Sul do Brasil. Uma das medidas de controle recomendadas é o uso de antagonistas. Desse modo, o objetivo desse trabalho foi avaliar o efeito de Trichoderma viride Pers. ex Gray sobre a incidência da doença. Um ensaio foi instalado em um plantio comercial de Pinus elliottii Engelm var. elliottii, localizado em Santa Maria do Oeste (PR. O inóculo do antagonista foi produzido em grãos de trigo esterilizados e colonizados por T. viride, em laboratório. Trinta dias antes da aplicação do antagonista, o solo foi desinfestado com 10 L de uma solução de formaldeído a 2%, por cova. Os grãos de trigo com T. viride foram aplicados na cova durante o plantio das mudas de pínus. O ensaio foi montado em parcelas com trezentas plantas com quatro tratamentos: formaldeído mais antagonista, somente formaldeído, somente antagonista e testemunha. A mortalidade de árvores foi quantificada durante 49 meses. A incidência foi menor na parcela onde se aplicou somente formaldeído (5,7 %, seguido pelos tratamentos somente antagonista (11,3 %, formaldeído mais antagonista (11,7 % e testemunha (12,3 %.

  4. Root anatomical phenes predict root penetration ability and biomechanical properties in maize (Zea Mays).

    Science.gov (United States)

    Chimungu, Joseph G; Loades, Kenneth W; Lynch, Jonathan P

    2015-06-01

    The ability of roots to penetrate hard soil is important for crop productivity but specific root phenes contributing to this ability are poorly understood. Root penetrability and biomechanical properties are likely to vary in the root system dependent on anatomical structure. No information is available to date on the influence of root anatomical phenes on root penetrability and biomechanics. Root penetration ability was evaluated using a wax layer system. Root tensile and bending strength were evaluated in plant roots grown in the greenhouse and in the field. Root anatomical phenes were found to be better predictors of root penetrability than root diameter per se and associated with smaller distal cortical region cell size. Smaller outer cortical region cells play an important role in stabilizing the root against ovalization and reducing the risk of local buckling and collapse during penetration, thereby increasing root penetration of hard layers. The use of stele diameter was found to be a better predictor of root tensile strength than root diameter. Cortical thickness, cortical cell count, cortical cell wall area and distal cortical cell size were stronger predictors of root bend strength than root diameter. Our results indicate that root anatomical phenes are important predictors for root penetrability of high-strength layers and root biomechanical properties. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  5. Selective Root Retreatment: A Novel Approach.

    Science.gov (United States)

    Nudera, William J

    2015-08-01

    Root canal retreatment is traditionally considered an "all or none" treatment approach. It is typically recommended that all restorative and obturation materials be removed from all roots regardless of the presence or absence of periapical pathosis. In contrast, surgical endodontics is not viewed as an "all or none" treatment approach. Traditionally, only the diseased root(s) is addressed via root-end resection and root-end filling. The use of cone-beam computed tomographic imaging allows for a more accurate evaluation of the periapical status of individual roots associated with multirooted teeth. This information has introduced a novel and conservative treatment alternative for previously endodontically treated teeth with multiple roots presenting with post-treatment disease. This new approach is termed selective root retreatment. Advanced imaging allows the clinician to make predictable treatment decisions with respect to the presence or absence of periapical pathosis of individual roots as opposed to making assumptions about the tooth as a whole. Selective root retreatment combines the approach of nonsurgical retreatment with the selectivity of surgical root resection. In this manner, retreatment could be limited to a single root or roots clearly showing periapical pathosis while leaving the root(s) with no visible or perceived pathosis untouched. Copyright © 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  6. Live cell imaging of Arabidopsis root hairs

    NARCIS (Netherlands)

    Ketelaar, T.

    2014-01-01

    Root hairs are tubular extensions from the root surface that expand by tip growth. This highly focused type of cell expansion, combined with position of root hairs on the surface of the root, makes them ideal cells for microscopic observation. This chapter describes the method that is routinely used

  7. MES buffer affects Arabidopsis root apex zonation and root growth by suppressing superoxide generation in root apex

    Directory of Open Access Journals (Sweden)

    Tomoko eKagenishi

    2016-02-01

    Full Text Available In plants, growth of roots and root hairs is regulated by the fine cellular control of pH and reactive oxygen species. MES, 2-(N-morpholinoethanesulfonic acid as one of the Good’s buffers has broadly been used for buffering medium, and it is thought to suit for plant growth with the concentration at 0.1% (w/v because the buffer capacity of MES ranging pH 5.5-7.0 (for Arabidopsis, pH 5.8. However, many reports have shown that, in nature, roots require different pH values on the surface of specific root apex zones, namely meristem, transition zone and elongation zone. Despite the fact that roots always grow on a media containing buffer molecule, little is known about impact of MES on root growth. Here, we have checked the effects of different concentrations of MES buffer using growing roots of Arabidopsis thaliana. Our results show that 1% of MES significantly inhibited root growth, the number of root hairs and length of meristem, whereas 0.1% promoted root growth and root apex area (region spanning from the root tip up to the transition zone. Furthermore, superoxide generation in root apex disappeared at 1% of MES. These results suggest that MES disturbs normal root morphogenesis by changing the reactive oxygen species (ROS homeostasis in root apex.

  8. MES Buffer Affects Arabidopsis Root Apex Zonation and Root Growth by Suppressing Superoxide Generation in Root Apex.

    Science.gov (United States)

    Kagenishi, Tomoko; Yokawa, Ken; Baluška, František

    2016-01-01

    In plants, growth of roots and root hairs is regulated by the fine cellular control of pH and reactive oxygen species (ROS). MES, 2-(N-morpholino)ethanesulfonic acid as one of the Good's buffers has broadly been used for buffering medium, and it is thought to suit for plant growth with the concentration at 0.1% (w/v) because the buffer capacity of MES ranging pH 5.5-7.0 (for Arabidopsis, pH 5.8). However, many reports have shown that, in nature, roots require different pH values on the surface of specific root apex zones, namely meristem, transition zone, and elongation zone. Despite the fact that roots always grow on a media containing buffer molecule, little is known about impact of MES on root growth. Here, we have checked the effects of different concentrations of MES buffer using growing roots of Arabidopsis thaliana. Our results show that 1% of MES significantly inhibited root growth, the number of root hairs and length of meristem, whereas 0.1% promoted root growth and root apex area (region spanning from the root tip up to the transition zone). Furthermore, superoxide generation in root apex disappeared at 1% of MES. These results suggest that MES disturbs normal root morphogenesis by changing the ROS homeostasis in root apex.

  9. Radiopacity of root filling materials

    International Nuclear Information System (INIS)

    Beyer-Olsen, E.M.

    1983-01-01

    A method for measuring the radiopacity of root filling materials is described. Direct measurements were made of the optic density values of the materials in comparison with a standard curve relating optic density to the thickness of an aluminium step wedge exposed simultaneously. By proper selection of film and conditions for exposure and development, it was possible to obtain a near-linear standard curve which added to the safety and reproducibility of the method. The technique of radiographic assessment was modified from clinical procedures in evaluating the obturation in radiographs, and it was aimed at detecting slits or voids between the dental wall and the filling material. This radiographic assessment of potensial leakage was compared with actual in vitro lekage of dye (basic fuchsin) into the roots of filled teeth. The result of the investigation show that root filling materials display a very wide range of radiopacity, from less than 3 mm to more than 12 mm of aluminium. It also seem that tooth roots that appear to be well obturated by radiographic evaluation, stand a good chance of beeing resistant to leakage in vitro, and that the type of filling material rather than its radiographic appearance, determines the susceptibility of the filled tooth to leakage in vitro. As an appendix the report contains a survey of radiopaque additives in root filling materials

  10. Imaging and modelling root water uptake

    Science.gov (United States)

    Zarebanadkouki, M.; Meunier, F.; Javaux, M.; Kaestner, A.; Carminati, A.

    2017-12-01

    Spatially resolved measurement and modelling of root water uptake is urgently needed to identify root traits that can improve capture of water from the soil. However, measuring water fluxes into roots of transpiring plants growing in soil remains challenging. Here, we describe an in-situ technique to measure local fluxes of water into roots. The technique consists of tracing the transport of deuterated water (D2O) in soil and roots using time series neutron radiography and tomography. A diffusion-convection model was used to model the transport of D2O in roots. The model includes root features such as the endodermis, xylem and the composite flow of water in the apoplastic and symplastic pathways. Diffusion permeability of root cells and of the endodermis were estimated by fitting the experiment during the night, when transpiration was negligible. The water fluxes at different position of the root system were obtained by fitting the experiments at daytime. The results showed that root water uptake was not uniform along root system and varied among different root types. The measured profiles of root water uptake into roots were used to estimate the radial and axial hydraulic of the roots. A three-dimensional model of root water uptake was used to fit the measured water fluxes by adjusting the root radial and axial hydraulic conductivities. We found that the estimated radial conductivities decreased with root age, while the axial conducances increased, and they are different among root types. The significance of this study is the development of a method to estimate 1) water uptake and 2) the radial and axial hydraulic conductivities of roots of transpiring plants growing in the soil.

  11. Variação da densidade anofélica com o uso de mosquiteiros impregnados com deltametrina em uma área endêmica de malária na Amazônia Brasileira Variation of anopheles density with deltamethrin-impregnated mosquito nets in an endemic malaria area of the Brazilian Amazon

    Directory of Open Access Journals (Sweden)

    João Barberino Santos

    1999-04-01

    Full Text Available Em 1992, foi realizado um ensaio de campo com mosquiteiros impregnados com deltametrina, em uma área endêmica de malária no Município de Costa Marques, Rondônia. No período de baixa transmissão, os mosquiteiros impregnados (MI diminuíram a densidade vetorial no intradomicílio, de modo semelhante, porém, aos mosquiteiros não impregnados (NI na maioria das avaliações-controle. Por outro lado, na época de alta transmissão, os MI provocaram diminuição significante da média horária de anofelinos capturados, ao passo que, nas casas com NI, verificou-se aumento do número de mosquitos capturados. No peridomicílio, em geral, não houve diferença do número de anofelinos capturados entre MI e NI. No grupo MI, a espécie mais capturada foi o An. darlingi (63,2%, mais freqüente no peridomicílio, seguida pelo An. deaneorum (35,3%, mais freqüente no intradomicílio. Os MI diminuíram a densidade vetorial no intradomicílio pelo efeito excito-repelente, sem diminuí-la, contudo, no peridomicílio.In 1992 a survey on the use of deltamethrin-impregnated mosquito nets was conducted in the municipality of Costa Marques, Rondonia. In the intradomicile, impregnated nets decreased the vector density at rates similar to those for non-impregnated nets during low-transmission periods; during high anopheline density periods, they led to a significant reduction in vector density, while in the non-impregnated net group there was an increase in the number of anophelines captured. There was no change in vector density in the peridomicile. In the impregnated net group, the most frequently captured species was Anopheles darlingi (63.2%, found mostly in the peridomicile, while Anopheles deaneorum (35.3% was most frequent indoors. The impregnated mosquito nets' excitatory-repellent effect decreased the intradomiciliary vector density but did not alter density in the peridomicile.

  12. ROOT VEGETABLES, BREEDING TRENDS, RESULTS

    Directory of Open Access Journals (Sweden)

    M. I. Fedorova

    2017-01-01

    Full Text Available The main advantage of root vegetables is their unique specificity and high economic importance. The benefits and medicinal properties of root vegetables being highly demanded by the market requirements to the commodity are highlighted in the article. The main directions of breeding program for root vegetable crops, including species of Apiaceae family with carrot, parsnips; Chenopodioideae family with red beet; Brassicaceae family with radish, Daikon, Raphanus sativus L. var. lobo Sazonova & Stank, turnip and rutabaga. Initial breeding accessions of carrot, red beet, radish, Daikon, Raphanus sativus L. var. lobo Sazonova & Stank, turnip and rutabaga have been selected out to be used for breeding program for heterosis. The mf and ms breeding lines were developed, and with the use of them the new gene pool was created. Variety supporting breeding program and methods were also proposed. 

  13. ROOT I/O improvements

    Energy Technology Data Exchange (ETDEWEB)

    Canal, Ph. [Fermilab

    2012-01-01

    In the past year, the development of ROOT I/O has focused on improving the existing code and increasing the collaboration with the experiments experts. Regular I/O workshops have been held to share and build upon the various experiences and points of view. The resulting improvements in ROOT I/O span many dimensions including reduction and more control over the memory usage, reduction in CPU usage as well as optimization of the file size and the hardware I/O utilization. Many of these enhancements came as a result of an increased collaboration with the experiments development teams and their direct contributions both in code and quarterly ROOT I/O workshops.

  14. Retention of Root Canal Posts

    DEFF Research Database (Denmark)

    Sahafi, A; Benetti, Ana Raquel; Flury, S

    2015-01-01

    The aim of this study was to investigate the effect of the cement film thickness of a zinc phosphate or a resin cement on retention of untreated and pretreated root canal posts. Prefabricated zirconia posts (CosmoPost: 1.4 mm) and two types of luting cements (a zinc phosphate cement [DeTrey Zinc......] and a self-etch adhesive resin cement [Panavia F2.0]) were used. After removal of the crowns of 360 extracted premolars, canines, or incisors, the root canals were prepared with a parallel-sided drill system to three different final diameters. Half the posts did not receive any pretreatment. The other half...... received tribochemical silicate coating according to the manufacturer's instructions. Posts were then luted in the prepared root canals (n=30 per group). Following water storage at 37°C for seven days, retention of the posts was determined by the pull-out method. Irrespective of the luting cement...

  15. Machine Learning Developments in ROOT

    Science.gov (United States)

    Bagoly, A.; Bevan, A.; Carnes, A.; Gleyzer, S. V.; Moneta, L.; Moudgil, A.; Pfreundschuh, S.; Stevenson, T.; Wunsch, S.; Zapata, O.

    2017-10-01

    ROOT is a software framework for large-scale data analysis that provides basic and advanced statistical methods used by high-energy physics experiments. It includes machine learning tools from the ROOT-integrated Toolkit for Multivariate Analysis (TMVA). We present several recent developments in TMVA, including a new modular design, new algorithms for pre-processing, cross-validation, hyperparameter-tuning, deep-learning and interfaces to other machine-learning software packages. TMVA is additionally integrated with Jupyter, making it accessible with a browser.

  16. Root justifications for ontology repair

    CSIR Research Space (South Africa)

    Moodley, K

    2011-08-01

    Full Text Available stream_source_info Moodley_2011.pdf.txt stream_content_type text/plain stream_size 32328 Content-Encoding ISO-8859-1 stream_name Moodley_2011.pdf.txt Content-Type text/plain; charset=ISO-8859-1 Root Justi cations... the ontology, based on the no- tion of root justi cations [8, 9]. In Section 5, we discuss the implementation of a Prot eg e3 plugin which demonstrates our approach to ontology repair. In this section we also discuss some experimental results comparing...

  17. Roots redefined: anatomical and genetic analysis of root development

    NARCIS (Netherlands)

    Scheres, B.J.G.; McKahnn, H.; Berg, C. van den

    1996-01-01

    The postembryonic development of plants is fueled by apical meristems, which are the local production sites of new cells that form a pattern of different cell types within an organ. The regularity of this pattern in the root yielded ideas on its formation from the meristem well before critical

  18. Nonsurgical management of horizontal root fracture associated external root resorption and internal root resorption

    Directory of Open Access Journals (Sweden)

    Shiraz Pasha

    2016-01-01

    Full Text Available Horizontal root fractures, which frequently affect the upper incisors, usually result from a frontal impact. As a result, combined injuries occur in dental tissues such as the pulp, dentin, cementum, periodontal ligament, and alveolar bone. Internal root canal inflammatory resorption involves a progressive loss of intraradicular dentin without adjunctive deposition of hard tissues adjacent to the resorptive sites. It is frequently associated with chronic pulpal inflammation, and bacteria might be identified from the granulation tissues when the lesion is progressive to the extent that it is identifiable with routine radiographs. With the advancement in technology, it is imperative to use modern diagnostic tools such as cone beam computed tomography and radiovisuography to diagnose and confirm the presence and extent of resorptions and fractures and their exact location. This case report presents a rare case having internal root resorption and horizontal root fracture with external inflammatory root resorption both which were treated successfully following guidelines by International Association of Dental Traumatology by nonsurgical treatment with 1 year follow-up.

  19. Neonatal Phosphate Nutrition Alters <em>in em>Vivo> and <em>in em>Vitro> Satellite Cell Activity in Pigs

    Directory of Open Access Journals (Sweden)

    Chad H. Stahl

    2012-05-01

    Full Text Available Satellite cell activity is necessary for postnatal skeletal muscle growth. Severe phosphate (PO4 deficiency can alter satellite cell activity, however the role of neonatal PO4 nutrition on satellite cell biology remains obscure. Twenty-one piglets (1 day of age, 1.8 ± 0.2 kg BW were pair-fed liquid diets that were either PO4 adequate (0.9% total P, supra-adequate (1.2% total P in PO4 requirement or deficient (0.7% total P in PO4 content for 12 days. Body weight was recorded daily and blood samples collected every 6 days. At day 12, pigs were orally dosed with BrdU and 12 h later, satellite cells were isolated. Satellite cells were also cultured <em>in vitroem> for 7 days to determine if PO4 nutrition alters their ability to proceed through their myogenic lineage. Dietary PO4 deficiency resulted in reduced (<em>P> < 0.05 sera PO4 and parathyroid hormone (PTH concentrations, while supra-adequate dietary PO4 improved (<em>P> < 0.05 feed conversion efficiency as compared to the PO4 adequate group. <em>In vivoem> satellite cell proliferation was reduced (<em>P> < 0.05 among the PO4 deficient pigs, and these cells had altered <em>in vitroem> expression of markers of myogenic progression. Further work to better understand early nutritional programming of satellite cells and the potential benefits of emphasizing early PO4 nutrition for future lean growth potential is warranted.

  20. Controlled short-circuiting MIG-MAG welding process and procedures applied to the root pass in pipeline construction; Processo de soldagem MIG/MAG em curto-circuito controlado e procedimentos aplicados ao passe de raiz na construcao de linhas dutoviarias

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Regis H.G. e; Gohr Junior, Raul; Weck, Leonardo W.A. [Santa Catarina Univ., Florianopolis, SC (Brazil). Dept. de Engenharia Mecanica. Lab. de Soldagem e Mecatronica (LABSOLDA)

    2005-07-01

    The work deals with the study and development of the Controlled Short-Circuiting MIG/MAG Welding Process (CCC) and procedures for the root pass on pipes, in pipelines construction. The developed process (CCC) consists in an semi-automatic slag free operation, yielding higher productivity than the Coated Electrode and TIG processes, with satisfactory properties on the root weld. The significant influence of the welding over the time schedule and construction cost makes the development of this technology attractive, in order to become available at low cost, enhancing the companies' competitiveness in the globalized oil sector. The developed system, a MIG/MAG variant, features the advantages of short-circuiting metal transfer and avoids its inconveniences (mainly with high CO{sub 2} content gases), enabling its use on pipes root welding. This is possible through current waveform control, providing process and weld pool stability. Procedures for the root pass were determined for each of the welding positions reached in thick walled pipes welding, with the CCC. Also, the low welder training time was notable. (author)

  1. Avaliação de técnica cirúrgica parendodôntica: apicectomia em 90º, retrocavitação com ultra-som e retrobturação com MTA = Evaluation of endodontics surgical technique: apical resection with a 90 o angle, ultrasonic preparation of root-end cavities and trioxide aggregate (MTA filling

    Directory of Open Access Journals (Sweden)

    Pozza, Daniel Humberto

    2005-01-01

    Full Text Available Técnicas consagradas como apicectomia por broca em 45 graus, retrocavidade com broca de aço esférica e retrobturação com amálgama tem sido gradativamente substituídas por novas seqüências de procedimentos que aumentam os benefícios cirúrgico, melhorando o prognóstico clínico. A metodologia utilizada neste artigo encontra-se ambasada em revisão bibliográfica e aplicação laboratorial. Isto possibilitou identificar inúmeras vantagens no uso da apicectomia em 90 graus, retrocavidade com ultra-som e retrobturação com Odontology Mineral Trioxide Aggregate (MTA, nas cirurgias

  2. Challenges to estimating whole forest root biomass with ground penetrating radar

    Science.gov (United States)

    Butnor, J. R.

    2016-12-01

    Over the past two decades, substantial technical advances have been made in detecting tree roots with ground penetrating radar (GPR). Under favorable soil dielectric conditions, root location, depth, diameter and mass estimates are possible in the field. With careful notation of survey lines, three dimensional reconstructions of root architecture may also be achieved. The technique has been very useful for quantifying lateral root biomass in silvicultural studies, but is not yet a standalone technique for estimating root biomass in forests. The purpose of this presentation is to highlight the limitations of GPR in the field to stimulate discussion on how to overcome these challenges. Under field conditions, surface-based antennas with frequencies of 400 to 1500 MHz cannot detect fine roots (detected. Lack of automation of data processing and interpretation steps currently makes data analysis arduous and in some cases subject to interpretation by an expert user. Forests have a high degree of heterogeneity in surface conditions (e.g., holes, soil moisture, stems, woody and herbaceous plants) that may prevent antennas from coupling with the surface to propagate EM waves and receive reflections. What is the potential for open source data analysis programs to be developed and shared? How will new digital, multi-frequency antennas improve resolution? Can air launched antennas be developed that have both the depth penetration and resolution to detect roots? Are purpose-designed bore hole antenna needed for imaging taproots?

  3. Root growth, secondary root formation and root gravitropism in carotenoid-deficient seedlings of Zea mays L

    Science.gov (United States)

    Ng, Y. K.; Moore, R.

    1985-01-01

    The effect of ABA on root growth, secondary-root formation and root gravitropism in seedlings of Zea mays was investigated by using Fluridone-treated seedlings and a viviparous mutant, both of which lack carotenoids and ABA. Primary roots of seedlings grown in the presence of Fluridone grew significantly slower than those of control (i.e. untreated) roots. Elongation of Fluridone-treated roots was inhibited significantly by the exogenous application of 1 mM ABA. Exogenous application of 1 micromole and 1 nmole ABA had either no effect or only a slight stimulatory effect on root elongation, depending on the method of application. The absence of ABA in Fluridone-treated plants was not an important factor in secondary-root formation in seedlings less than 9-10 d old. However, ABA may suppress secondary-root formation in older seedlings, since 11-d-old control seedlings had significantly fewer secondary roots than Fluridone-treated seedlings. Roots of Fluridone-treated and control seedlings were graviresponsive. Similar data were obtained for vp-9 mutants of Z. mays, which are phenotypically identical to Fluridone-treated seedlings. These results indicate that ABA is necessary for neither secondary-root formation nor for positive gravitropism by primary roots.

  4. MAIL1 is essential for development of the primary root but not of anchor roots

    OpenAIRE

    Ühlken, Christine; Hoth, Stefan; Weingartner, Magdalena

    2014-01-01

    MAIN-LIKE1 (MAIL1) is a ubiquitously expressed nuclear protein, which has a crucial function during root development. We have recently described loss of function mutants for MAIL1, in which the organization and function of the primary root meristem is lost soon after germination. Moreover cell differentiation is impaired resulting in primary root growth arrest soon after emergence. Here we show that mail1 mutants form several anchor roots from the hypocotyl to root junction. These anchor root...

  5. Study on Root Canal Preparation with Endo Motor Tecnika Vision and Sealing of Root Canal Filing

    OpenAIRE

    藤原, 治; 佐々木, 重夫; 天野, 義和; フジワラ, オサム; ササキ, シゲオ; アマノ, ヨシカズ; Osamu, FUJIWARA; Shigeo, SASAKI; Yoshikazu, AMANO

    2008-01-01

    Because root canal preparation in root canal treatment takes long time, various special contraangle handpiece are developed to promote efficiency of root canal preparation. Root canal are prepared with computerized micro motor handpiece. The purpose of this study is to examine the time of root canal preparation and the difference of sealing efficiency of root canal fillings between exclusive point and other point after preparation in the cases of files Endo and motor technika vision, and to f...

  6. Maximal abelian sets of roots

    CERN Document Server

    Lawther, R

    2018-01-01

    In this work the author lets \\Phi be an irreducible root system, with Coxeter group W. He considers subsets of \\Phi which are abelian, meaning that no two roots in the set have sum in \\Phi \\cup \\{ 0 \\}. He classifies all maximal abelian sets (i.e., abelian sets properly contained in no other) up to the action of W: for each W-orbit of maximal abelian sets we provide an explicit representative X, identify the (setwise) stabilizer W_X of X in W, and decompose X into W_X-orbits. Abelian sets of roots are closely related to abelian unipotent subgroups of simple algebraic groups, and thus to abelian p-subgroups of finite groups of Lie type over fields of characteristic p. Parts of the work presented here have been used to confirm the p-rank of E_8(p^n), and (somewhat unexpectedly) to obtain for the first time the 2-ranks of the Monster and Baby Monster sporadic groups, together with the double cover of the latter. Root systems of classical type are dealt with quickly here; the vast majority of the present work con...

  7. Maximal Abelian sets of roots

    CERN Document Server

    Lawther, R

    2018-01-01

    In this work the author lets \\Phi be an irreducible root system, with Coxeter group W. He considers subsets of \\Phi which are abelian, meaning that no two roots in the set have sum in \\Phi \\cup \\{ 0 \\}. He classifies all maximal abelian sets (i.e., abelian sets properly contained in no other) up to the action of W: for each W-orbit of maximal abelian sets we provide an explicit representative X, identify the (setwise) stabilizer W_X of X in W, and decompose X into W_X-orbits. Abelian sets of roots are closely related to abelian unipotent subgroups of simple algebraic groups, and thus to abelian p-subgroups of finite groups of Lie type over fields of characteristic p. Parts of the work presented here have been used to confirm the p-rank of E_8(p^n), and (somewhat unexpectedly) to obtain for the first time the 2-ranks of the Monster and Baby Monster sporadic groups, together with the double cover of the latter. Root systems of classical type are dealt with quickly here; the vast majority of the present work con...

  8. Disease notes - Bacterial root rot

    Science.gov (United States)

    Bacterial root rot initiated by lactic acid bacteria, particularly Leuconostoc, occurs every year in Idaho sugarbeet fields. Hot fall weather seems to make the problem worse. Although Leuconostoc initiates the rot, other bacteria and yeast frequently invade the tissue as well. The acetic acid bac...

  9. Root Asymptotics of Spectral Polynomials

    Czech Academy of Sciences Publication Activity Database

    Shapiro, B.; Tater, Miloš

    2007-01-01

    Roč. 47, č. 2 (2007), s. 33-36 ISSN 0355-2721 R&D Projects: GA MŠk LC06002 Institutional research plan: CEZ:AV0Z10480505 Keywords : asymptotic root -counting measure Subject RIV: BA - General Mathematics

  10. [Effects nutrients on the seedlings root hair development and root growth of Poncirus trifoliata under hydroponics condition].

    Science.gov (United States)

    Cao, Xiu; Xia, Ren-Xue; Zhang, De-Jian; Shu, Bo

    2013-06-01

    Ahydroponics experiment was conducted to study the effects of nutrients (N, P, K, Ca, Mg, Fe, and Mn) deficiency on the length of primary root, the number of lateral roots, and the root hair density, length, and diameter on the primary root and lateral roots of Poncirus trifoliata seedlings. Under the deficiency of each test nutrient, root hair could generate, but was mainly concentrated on the root base and fewer on the root tip. The root hair density on lateral roots was significantly larger than that on primary root, but the root hair length was in adverse. The deficiency of each test nutrient had greater effects on the growth and development of root hairs, with the root hair density on primary root varied from 55.0 to 174.3 mm(-2). As compared with the control, Ca deficiency induced the significant increase of root hair density and length on primary root, P deficiency promoted the root hair density and length on the base and middle part of primary root and on the lateral roots significantly, Fe deficiency increased the root hair density but decreased the root hair length on the tip of primary root significantly, K deficiency significantly decreased the root hair density, length, and diameter on primary root and lateral roots, whereas Mg deficiency increased the root hair length of primary root significantly. In all treatments of nutrient deficiency, the primary root had the similar growth rate, but, with the exceptions of N and Mg deficiency, the lateral roots exhibited shedding and regeneration.

  11. Constituents from <em>Vigna em>vexillata> and Their Anti-Inflammatory Activity

    Directory of Open Access Journals (Sweden)

    Guo-Feng Chen

    2012-08-01

    Full Text Available The seeds of <em>Vigna em>genus are important food resources and there have already been many reports regarding their bioactivities. In our preliminary bioassay, the chloroform layer of methanol extracts of<em> V. vexillata em>demonstrated significant anti-inflammatory bioactivity. Therefore, the present research is aimed to purify and identify the anti-inflammatory principles of <em>V. vexillataem>. One new sterol (1 and two new isoflavones (2,3 were reported from the natural sources for the first time and their chemical structures were determined by the spectroscopic and mass spectrometric analyses. In addition, 37 known compounds were identified by comparison of their physical and spectroscopic data with those reported in the literature. Among the isolates, daidzein (23, abscisic acid (25, and quercetin (40 displayed the most significant inhibition of superoxide anion generation and elastase release.

  12. Investigation of VEGGIE Root Mat

    Science.gov (United States)

    Subbiah, Arun M.

    2013-01-01

    VEGGIE is a plant growth facility that utilizes the phenomenon of capillary action as its primary watering system. A cloth made of Meta Aramid fiber, known as Nomex is used to wick water up from a reservoir to the bottom of the plants roots. This root mat system is intended to be low maintenance with no moving parts and requires minimal crew interface time. Unfortunately, the water wicking rates are inconsistent throughout the plant life cycle, thus causing plants to die. Over-wicking of water occurs toward the beginning of the cycle, while under-wicking occurs toward the middle. This inconsistency of wicking has become a major issue, drastically inhibiting plant growth. The primary objective is to determine the root cause of the inconsistent wicking through experimental testing. Suspect causes for the capillary water column to break include: a vacuum effect due to a negative pressure gradient in the water reservoir, contamination of material due to minerals in water and back wash from plant fertilizer, induced air bubbles while using syringe refill method, and material limitations of Nomex's ability to absorb and retain water. Experimental testing will be conducted to systematically determine the cause of under and over-wicking. Pressure gages will be used to determine pressure drop during the course of the plant life cycle and during the water refill process. A debubbler device will be connected to a root mat in order to equalize pressure inside the reservoir. Moisture and evaporation tests will simultaneously be implemented to observe moisture content and wicking rates over the course of a plant cycle. Water retention tests will be performed using strips of Nomex to determine materials wicking rates, porosity, and absorptivity. Through these experimental tests, we will have a better understanding of material properties of Nomex, as well as determine the root cause of water column breakage. With consistent test results, a forward plan can be achieved to resolve

  13. Characterizing pathways by which gravitropic effectors could move from the root cap to the root of primary roots of Zea mays

    Science.gov (United States)

    Moore, R.; McClelen, C. E.

    1989-01-01

    Plasmodesmata linking the root cap and root in primary roots Zea mays are restricted to approx. 400 protodermal cells bordering approx. 110000 microns2 of the calyptrogen of the root cap. This area is less than 10% of the cross-sectional area of the root-tip at the cap junction. Therefore, gravitropic effectors moving from the root cap to the root can move symplastically only through a relatively small area in the centre of the root. Decapped roots are non-responsive to gravity. However, decapped roots whose caps are replaced immediately after decapping are strongly graviresponsive. Thus, gravicurvature occurs only when the root cap contacts the root, and symplastic continuity between the cap and root is not required for gravicurvature. Completely removing mucilage from the root tip renders the root non-responsive to gravity. Taken together, these data suggest that gravitropic effectors move apoplastically through mucilage from the cap to the root.

  14. Organic acid effect on calcium uptake by the wheat roots

    Directory of Open Access Journals (Sweden)

    Fabrize Caroline Nunes

    2009-02-01

    Full Text Available This work evaluated the effect of the natural organic acids on the uptake of Ca by the wheat roots in a hydroponic solution. The following organic acids were evaluated: citric, oxalic, tartaric, malic, malonic, maleic, DL-malic, p-hydroxybenzoic, aconitic, and salicilic. The organic acids neither enhanced the root growth nor increased Ca uptake. The salicilic and malic acids were highly toxic and decreased the root growth. The citric, tartaric, maleic, aconitic, and salicilic decreased the Ca uptake by the roots due to their higher capacity to form the stable complexes with Ca in solution at pH 6.0. Decreasing the Ca valence from Ca++ to CaL+ or CaL2(0 through the organic ligand complexation reactions decreased the Ca uptake. The results suggested that the wheat roots do not absorb Ca-organic complexes.Ácidos orgânicos possuem grupos funcionais com cargas negativas que complexam íons metálicos em solução. Este trabalho avaliou o efeito de ácidos orgânicos naturais na absorção de Ca pelas raízes de trigo. Foram avaliados os seguintes ácidos orgânicos: cítrico, oxálico, tartarico, málico, malônico, maleico, DL-málico, p-hidroxibenzoico, aconítico e salicílico. Os ácidos orgânicos não estimularam o crescimento das raízes e não aumentaram a absorção de Ca. Os ácidos salicílico e maleico diminuíram drasticamente o crescimento radicular. Os ácidos cítrico, tartárico, maleico, aconítico e salicílico diminuíram a absorção de Ca pelas raízes devido à maior capacidade de formar complexos estáveis com Ca em solução no pH 6,0. A redução da valência de Ca++ para CaL+ e CaL2(0, através das reações de complexação, diminuiu a absorção de Ca pelas raízes. Os resultados sugerem que os complexos de Ca-orgânico não são absorvidos pelas raízes de trigo.

  15. Effect of Calcium and Potassium on Antioxidant System of <em>Vicia fabaem> L. Under Cadmium Stress

    Directory of Open Access Journals (Sweden)

    Hayssam M. Ali

    2012-05-01

    Full Text Available Cadmium (Cd in soil poses a major threat to plant growth and productivity. In the present experiment, we studied the effect of calcium (Ca2+ and/or potassium (K+ on the antioxidant system, accumulation of proline (Pro, malondialdehyde (MDA, and content of photosynthetic pigments, cadmium (Cd and nutrients, <em>i.e.>, Ca2+ and K+ in leaf of <em>Vicia faba em>L. (cv. TARA under Cd stress. Plants grown in the presence of Cd exhibited reduced growth traits [root length (RL plant−1, shoot length (SL plant−1, root fresh weight (RFW plant−1, shoot fresh weight (SFW plant−1, root dry weight (RDW plant−1 and shoot dry weight (SDW plant−1] and concentration of Ca2+, K+, Chlorophyll (Chl <em>a> and Chl <em>b em>content, except content of MDA, Cd and (Pro. The antioxidant enzymes [peroxidase (POD and superoxide dismutase (SOD] slightly increased as compared to control under Cd stress. However, a significant improvement was observed in all growth traits and content of Ca2+, K+, Chl <em>a>, Chl <em>b em>,Pro and activity of antioxidant enzymes catalase (CAT, POD and SOD in plants subjected to Ca2+ and/or K+. The maximum alleviating effect was recorded in the plants grown in medium containing Ca2+ and K+ together. This study indicates that the application of Ca2+ and/or K+ had a significant and synergistic effect on plant growth. Also, application of Ca2+ and/or K+ was highly effective against the toxicity of Cd by improving activity of antioxidant enzymes and solute that led to the enhanced plant growth of faba bean plants.

  16. Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH2 pastes Aspectos histomicrobiológicos do sistema de canais radiculares e das lesões periapicais em dentes de cães após instrumentação automatizada e medicação intracanal com pastas de Ca(OH2

    Directory of Open Access Journals (Sweden)

    Janir Alves Soares

    2006-10-01

    Full Text Available OBJECTIVE: The purpose of this study was to evaluate the distribution of microorganisms in the root canal system (RCS and periapical lesions of dogs' teeth after rotary instrumentation and placement of different calcium hydroxide [Ca(OH2]-based intracanal dressings. MATERIALS AND METHODS: Chronic periapical lesions were experimentally induced in 80 premolar roots of four dogs. Instrumentation was undertaken using the ProFile rotary system and irrigation with 5.25% sodium hypochlorite. The following Ca(OH2-based pastes were applied for 21 days: group 1 - Calen (n=18; group 2 - Calen+CPMC (n=20; group 3 - Ca(OH2 p.a. + anaesthetic solution (n=16 and group 4 - Ca(OH2 p.a.+ 2% chlorhexidine digluconate (n=18. Eight root canals without endodontic treatment constituted the control group. Histological sections were obtained and stained with Brown & Brenn staining technique to evaluate the presence of microorganisms in the main root canal, ramifications of the apical delta and secondary canals, apical cementoplasts, dentinal tubules, areas of cemental resorption and periapical lesions. The results were analyzed statistically by the Mann-Whitney U test (p0.05. The percentage of RCS sites containing microorganisms in groups 1, 2, 3, 4 and control were: 67.6%, 62.5%, 78.2%, 62.0% and 87.6%, respectively. CONCLUSION: In conclusion, the histomicrobiological analysis showed that the rotary instrumentation and the different calcium hydroxide pastes employed did not effectively eliminate the infection from the RCS and periapical lesions. However, several bacteria seen in the histological sections were probably dead or were inactivated by the biomechanical preparation and calcium hydroxide-based intracanal dressing.OBJETIVO: Neste estudo avaliou-se a distribuição dos microrganismos no sistema de canais radiculares (SCR e região periapical (RP de cães após instrumentação automatizada e utilização de pastas à base de hidróxido de cálcio CaO(H2

  17. MAIL1 is essential for development of the primary root but not of anchor roots.

    Science.gov (United States)

    Ühlken, Christine; Hoth, Stefan; Weingartner, Magdalena

    2014-01-01

    MAIN-LIKE1 (MAIL1) is a ubiquitously expressed nuclear protein, which has a crucial function during root development. We have recently described loss of function mutants for MAIL1, in which the organization and function of the primary root meristem is lost soon after germination. Moreover cell differentiation is impaired resulting in primary root growth arrest soon after emergence. Here we show that mail1 mutants form several anchor roots from the hypocotyl to root junction. These anchor roots show similar defects in the organization of the stem cell niche as the primary root. In contrast, differentiation processes are not impaired and thus anchor roots seem to be able to compensate for the loss of primary root function. Our data show that MAIL1 is essential for specification of cell fate in the primary root but not in anchor roots.

  18. Light and decapitation effects on in vitro rooting in maize root segments.

    Science.gov (United States)

    Golaz, F W; Pilet, P E

    1985-10-01

    The effects of white light and decapitation on the initiation and subsequent emergence and elongation of lateral roots of apical maize (Zea mays L. cv LG 11) root segments have been examined. The formation of lateral root primordium was inhibited by the white light. This inhibition did not depend upon the presence of the primary root tip. However, root decapitation induced a shift of the site of appearance of the most apical primordium towards the root apex, and a strong disturbance of the distribution pattern of primordium volumes along the root axis. White light had a significant effect neither on the distribution pattern of primordium volumes, nor on the period of primordium development (time interval required for the smallest detectable primordia to grow out as secondary roots). Thus, considering the rooting initiation and emergence, the light effect was restricted to the initiation phase only. Moreover, white light reduced lateral root elongation as well as primary root growth.

  19. Cultivated method of short root american ginseng

    International Nuclear Information System (INIS)

    Chen Guang; Yuan Yuchun; Jia Zhifa; Suo Binhua

    1998-01-01

    The distribution rate of 14 C assimilated material and root vitality of two years old American ginseng at green seed stage were measured. An exploratory research was made by cutting part of main root and spraying ABT on leaves of American ginseng. The results show that with cutting part of main root out before transplant and then sticking them in the seed bed, the plant develop and grow normally and the lateral and fibrous roots grow well. Spraying ABT on leaves of the plant at seed forming stage accelerate the transfer of assimilated material to the root and enhance the root vitality, especially the lateral root vitality. It is considered that cutting part of main root out is major method and spraying ABT on leaves is a supplementary measurement

  20. Iterative roots of homeomorphisms possessing periodic points

    Directory of Open Access Journals (Sweden)

    Paweł Solarz

    2007-06-01

    Full Text Available In this paper we give necessary and sufficient conditions for the existence of orientation-preserving iterative roots of a homeomorphism with a nonempty set of periodic points. We also give a construction method for these roots.

  1. Root morphology of Ni-treated plants

    International Nuclear Information System (INIS)

    Leskova, A.; Fargasova, A.; Giehl, R. F. H.; Wiren, N. von

    2015-01-01

    Plant roots are very important organs in terms of nutrient and water acquisition but they also serve as anchorages for the aboveground parts of the plants. The roots display extraordinary plasticity towards stress conditions as a result of integration of environmental cues into the developmental processes of the roots. Our aim was to investigate the root morphology of Arabidopsis thaliana plants exposed to a particular stress condition, excess Ni supply. We aimed to find out which cellular processes - cell division, elongation and differentiation are affected by Ni, thereby explaining the seen root phenotype. Our results reveal that a distinct sensitivity exists between roots of different order and interference with various cellular processes is responsible for the effects of Ni on roots. We also show that Ni-treated roots have several auxin-related phenotypes. (authors)

  2. The Physiology of Adventitious Roots1

    Science.gov (United States)

    Steffens, Bianka; Rasmussen, Amanda

    2016-01-01

    Adventitious roots are plant roots that form from any nonroot tissue and are produced both during normal development (crown roots on cereals and nodal roots on strawberry [Fragaria spp.]) and in response to stress conditions, such as flooding, nutrient deprivation, and wounding. They are important economically (for cuttings and food production), ecologically (environmental stress response), and for human existence (food production). To improve sustainable food production under environmentally extreme conditions, it is important to understand the adventitious root development of crops both in normal and stressed conditions. Therefore, understanding the regulation and physiology of adventitious root formation is critical for breeding programs. Recent work shows that different adventitious root types are regulated differently, and here, we propose clear definitions of these classes. We use three case studies to summarize the physiology of adventitious root development in response to flooding (case study 1), nutrient deficiency (case study 2), and wounding (case study 3). PMID:26697895

  3. Rhizosphere biophysics and root water uptake

    Science.gov (United States)

    Carminati, Andrea; Zarebanadkouki, Mohsen; Ahmed, Mutez A.; Passioura, John

    2016-04-01

    The flow of water into the roots and the (putative) presence of a large resistance at the root-soil interface have attracted the attention of plant and soil scientists for decades. Such resistance has been attributed to a partial contact between roots and soil, large gradients in soil matric potential around the roots, or accumulation of solutes at the root surface creating a negative osmotic potential. Our hypothesis is that roots are capable of altering the biophysical properties of the soil around the roots, the rhizosphere, facilitating root water uptake in dry soils. In particular, we expect that root hairs and mucilage optimally connect the roots to the soil maintaining the hydraulic continuity across the rhizosphere. Using a pressure chamber apparatus we measured the relation between transpiration rate and the water potential difference between soil and leaf xylem during drying cycles in barley mutants with and without root hairs. The samples were grown in well structured soils. At low soil moistures and high transpiration rates, large drops in water potential developed around the roots. These drops in water potential recovered very slowly, even after transpiration was severely decreased. The drops in water potential were much bigger in barley mutants without root hairs. These mutants failed to sustain high transpiration rates in dry conditions. To explain the nature of such drops in water potential across the rhizosphere we performed high resolution neutron tomography of the rhizosphere of the barleys with and without root hairs growing in the same soil described above. The tomograms suggested that the hydraulic contact between the soil structures was the highest resistance for the water flow in dry conditions. The tomograms also indicate that root hairs and mucilage improved the hydraulic contact between roots and soil structures. At high transpiration rates and low water contents, roots extracted water from the rhizosphere, while the bulk soil, due its

  4. Intersect a quartic to extract its roots

    OpenAIRE

    Raghavendra G. Kulkarni

    2017-01-01

    In this note we present a new method for determining the roots of a quartic polynomial, wherein the given quartic polynomial is intersected by a quadratic polynomial (which has two unknown coefficients) at its root point; so the root satisfies both the quartic and the quadratic equations. Elimination of the root term from the two equations leads to an expression in the two unknowns of quadratic polynomial. In addition, we introduce another expression in one unknown, which leads to determinati...

  5. Global Distribution of Root Nutrient Concentrations in Terrestrial Ecosystems

    Data.gov (United States)

    National Aeronautics and Space Administration — Nutrient measurements for fine roots were compiled from 56 published studies providing information on 372 different combinations of species, root diameter, rooting...

  6. Root proliferation in decaying roots and old root channels: A nutrient conservation mechanism in oligotrophic mangrove forests?

    Science.gov (United States)

    McKee, K.L.

    2001-01-01

    1. In oligotrophic habitats, proliferation of roots in nutrient-rich microsites may contribute to overall nutrient conservation by plants. Peat-based soils on mangrove islands in Belize are characterized by the presence of decaying roots and numerous old root channels (0.1-3.5 cm diameter) that become filled with living and highly branched roots of Rhizophora mangle and Avicennia germinans. The objectives of this study were to quantify the proliferation of roots in these microsites and to determine what causes this response. 2. Channels formed by the refractory remains of mangrove roots accounted for only 1-2% of total soil volume, but the proportion of roots found within channels varied from 9 to 24% of total live mass. Successive generations of roots growing inside increasingly smaller root channels were also found. 3. When artificial channels constructed of PVC pipe were buried in the peat for 2 years, those filled with nutrient-rich organic matter had six times more roots than empty or sand-filled channels, indicating a response to greater nutrient availability rather than to greater space or less impedance to root growth. 4. Root proliferation inside decaying roots may improve recovery of nutrients released from decomposing tissues before they can be leached or immobilized in this intertidal environment. Greatest root proliferation in channels occurred in interior forest zones characterized by greater soil waterlogging, which suggests that this may be a strategy for nutrient capture that minimizes oxygen losses from the whole root system. 5. Improved efficiency of nutrient acquisition at the individual plant level has implications for nutrient economy at the ecosystem level and may explain, in part, how mangroves persist and grow in nutrient-poor environments.

  7. Automatic schema evolution in Root

    International Nuclear Information System (INIS)

    Brun, R.; Rademakers, F.

    2001-01-01

    ROOT version 3 (spring 2001) supports automatic class schema evolution. In addition this version also produces files that are self-describing. This is achieved by storing in each file a record with the description of all the persistent classes in the file. Being self-describing guarantees that a file can always be read later, its structure browsed and objects inspected, also when the library with the compiled code of these classes is missing. The schema evolution mechanism supports the frequent case when multiple data sets generated with many different class versions must be analyzed in the same session. ROOT supports the automatic generation of C++ code describing the data objects in a file

  8. Root Cause Analysis: Methods and Mindsets.

    Science.gov (United States)

    Kluch, Jacob H.

    This instructional unit is intended for use in training operations personnel and others involved in scram analysis at nuclear power plants in the techniques of root cause analysis. Four lessons are included. The first lesson provides an overview of the goals and benefits of the root cause analysis method. Root cause analysis techniques are covered…

  9. A note on some iterative roots

    Directory of Open Access Journals (Sweden)

    Paweł Solarz

    2009-04-01

    Full Text Available In this paper some orientation-preserving iterative roots of an orientation-preserving homeomorphism $Fcolon S^1o S^1$ which possess periodic points of order $n$ are considered. Namely, iterative roots with periodic points of order $n$. All orders of such roots are determined and their general construction is given.

  10. Layers of root nouns in Germanic

    DEFF Research Database (Denmark)

    Hansen, Bjarne Simmelkjær Sandgaard

    2017-01-01

    The root-noun declension became productive in early Germanic, containing (I) inherited root nouns, (IIa) original substrate or loan words, and transitions from other declensions in (IIb) Proto-Germanic and (III) North Germanic. As ablaut was abolished, the inherited type would display ablaut grades...... that, in late Proto-Germanic, became predictable from the phonotactic structure of the root....

  11. Applications of root cause analysis

    International Nuclear Information System (INIS)

    Satterwhite, D.G.; Meale, B.M.; Krantz, E.A.

    1986-01-01

    The underlying causes for the failure of components, the root causes, can be obtained from operational data sources. This information is of value in focusing attention of the industry on the actual causes of component unavailability and, therefore, on the important contributors to plant risk. An application of this methodology to an actual plant system, and the results of this study, are presented in this paper

  12. Root finding with threshold circuits

    Czech Academy of Sciences Publication Activity Database

    Jeřábek, Emil

    2012-01-01

    Roč. 462, Nov 30 (2012), s. 59-69 ISSN 0304-3975 R&D Projects: GA AV ČR IAA100190902; GA MŠk(CZ) 1M0545 Institutional support: RVO:67985840 Keywords : root finding * threshold circuit * power series Subject RIV: BA - General Mathematics Impact factor: 0.489, year: 2012 http://www.sciencedirect.com/science/article/pii/S0304397512008006#

  13. Evaluation of bacterial leakage of four root- end filling materials: Gray Pro Root MTA, White Pro Root MTA, Root MTA and Portland Cement (type I

    Directory of Open Access Journals (Sweden)

    Zarabian M.

    2005-07-01

    Full Text Available Background and Aim: Today several materials have been used for root- end filling in endodontic surgery. Optimal properties of Pro Root MTA in in-vitro and in-vivo studies has been proven. On the other hand, based on some studies, Root MTA (Iranian Pro Root MTA and Portland cement are similar to Pro Root MTA in physical and biologic properties. The aim of this study was to evaluate bacterial leakage (amount and mean leakage time of four root- end filling materials. Materials and Methods: In this experimental in-vitro study, seventy six extracted single- rooted human teeth were randomly divided into six groups for root-end filling with gray Pro Root MTA, white Pro Root MTA, Root MTA (Iranian Pro Root MTA, Portland Cement (type I and positive and negative control groups. Root canals were instrumented using the step- back technique. Root- end filling materials were placed in 3mm ultra sonic retro preparations. Samples and microleakage model system were sterilized in autoclave. The apical 3-4 mm of the roots were immersed in phenol red with 3% lactose broth culture medium. The coronal access of each specimen was inoculated every 24h with a suspension of Streptococcus sanguis (ATCC 10556. Culture media were observed every 24h for colour change indicating bacterial contamination for 60 days. Statistical analysis was performed using log- rank test with P<0.05 as the limit of significance. Results: At the end of study 50%, 56.25%, 56.25% and 50% of specimens filled with Gray Pro Root MTA, White Pro Root MTA. Root MTA and Portland Cement (type I had evidence of leakage respectively. The mean leakage time was 37.19±6.29, 36.44±5.81, 37.69±5.97 and 34.81±6.67 days respectively. Statistical analysis of data showed no significant difference among the leakage (amount and mean leakage time of the four tested root- end filling materials (P=0.9958. Conclusion: Based on the results of this study, there were no significant differences in leakage among the four

  14. Induction of micronuclei in maize root-tip cells and a correlation with forward mutation at the yg2 locus

    International Nuclear Information System (INIS)

    Wagner, E.D.; Plewa, M.J.

    1985-01-01

    A standard protocol for a micronucleus assay in root tips of Zea mays was developed and calibrated using 137 Cs gamma radiation, ethylmethanesulfonate (EMS), and N-ethyl-N-nitrosourea (ENU). The root tips were sampled 1,3, and 5 days after the treatment period. Primary root tips were the most responsive with the highest frequencies of micronuclei. The frequencies then decreased to control values after 3 days. By treating the same kernels for this assay and the yellow-green-2 (yg2) forward mutation assay, it was possible to analyze and compare the induction kinetics of micronuclei formation in root-tip cells and forward mutation at the yg2 locus in leaf primordial cells. Forward mutation at the yg2 locus by gamma rays, EMS, or ENU exhibited one hit kinetics. The induction of micronuclei demonstrated nonlinear kinetics with a significant increase over controls above 200 rads gamma rays, 10 mM EMS, or 250 μM ENU. The data suggest that a higher relative proportion of yg2 mutant sectors induced by ENU may be due to chromosome aberrations than those induced by gamma radiation or EMS

  15. Printing Values In Interactive ROOT

    CERN Document Server

    Perovic, Boris

    2015-01-01

    This project report summarizes the work I have been performing during the past twelve weeks as a Summer Student intern working on ROOT project in the SFT group, PH department, under the supervision of Axel Naumann and Danilo Piparo. One of the widely requested features for ROOT was improved interactive shell experience as well as improved printing of object values. Solving this issue was the goal of this project. Primarily, we have enabled printing of the collections. Secondly, we have unified the printing interface, making it much more robust and extendible. Thirdly, we have implemented printing of nested collections in a flexible and user-friendly manner. Finally, we have added an interactive mode, allowing for paginated output. At the beginning of the report, ROOT is presented with examples of where it is used and how important it is. Then, the motivation behind the project is elaborated, by presenting the previous state of the software package and its potential for improvement. Further, the process in wh...

  16. Phene synergism between root hair length and basal root growth angle for phosphorus acquisition.

    Science.gov (United States)

    Miguel, Magalhaes Amade; Postma, Johannes Auke; Lynch, Jonathan Paul

    2015-04-01

    Shallow basal root growth angle (BRGA) increases phosphorus acquisition efficiency by enhancing topsoil foraging because in most soils, phosphorus is concentrated in the topsoil. Root hair length and density (RHL/D) increase phosphorus acquisition by expanding the soil volume subject to phosphorus depletion through diffusion. We hypothesized that shallow BRGA and large RHL/D are synergetic for phosphorus acquisition, meaning that their combined effect is greater than the sum of their individual effects. To evaluate this hypothesis, phosphorus acquisition in the field in Mozambique was compared among recombinant inbred lines of common bean (Phaseolus vulgaris) having four distinct root phenotypes: long root hairs and shallow basal roots, long root hairs and deep basal roots, short root hairs and shallow basal roots, and short root hairs and deep basal roots. The results revealed substantial synergism between BRGA and RHL/D. Compared with short-haired, deep-rooted phenotypes, long root hairs increased shoot biomass under phosphorus stress by 89%, while shallow roots increased shoot biomass by 58%. Genotypes with both long root hairs and shallow roots had 298% greater biomass accumulation than short-haired, deep-rooted phenotypes. Therefore, the utility of shallow basal roots and long root hairs for phosphorus acquisition in combination is twice as large as their additive effects. We conclude that the anatomical phene of long, dense root hairs and the architectural phene of shallower basal root growth are synergetic for phosphorus acquisition. Phene synergism may be common in plant biology and can have substantial importance for plant fitness, as shown here. © 2015 American Society of Plant Biologists. All Rights Reserved.

  17. Dermatoses em renais cronicos em terapia dialitica

    Directory of Open Access Journals (Sweden)

    Luis Alberto Batista Peres

    2014-03-01

    Full Text Available Objetivo: As desordens cutâneas e das mucosas são comuns em pacientes em hemodiálise a longo prazo. A diálise prolonga a expectativa de vida, dando tempo para a manifestação destas anormalidades. Os objetivos deste estudo foram avaliar a prevalência de problemas dermatológicos em pacientes com doença renal crônica (DRC em hemodiálise. Métodos: Cento e quarenta e cinco pacientes com doença renal crônica em hemodiálise foram estudados. Todos os pacientes foram completamente analisados para as alterações cutâneas, de cabelos, mucosas e unhas por um único examinador e foram coletados dados de exames laboratoriais. Os dados foram armazenados em um banco de dados do Microsolft Excel e analisados por estatística descritiva. As variáveis contínuas foram comparadas pelo teste t de Student e as variáveis categóricas utilizando o teste do qui-quadrado ou o teste Exato de Fischer, conforme adequado. Resultados: O estudo incluiu 145 pacientes, com idade média de 53,6 ± 14,7 anos, predominantemente do sexo masculino (64,1% e caucasianos (90,0%. O tempo médio de diálise foi de 43,3 ± 42,3 meses. As principais doenças subjacentes foram: hipertensão arterial em 33,8%, diabetes mellitus em 29,6% e glomerulonefrite crônica em 13,1%. As principais manifestações dermatológicas observadas foram: xerose em 109 (75,2%, equimose em 87 (60,0%, prurido em 78 (53,8% e lentigo em 33 (22,8% pacientes. Conclusão: O nosso estudo mostrou a presença de mais do que uma dermatose por paciente. As alterações cutâneas são frequentes em pacientes em diálise. Mais estudos são necessários para melhor caracterização e manejo destas dermatoses.

  18. Influence of substrate and mycorrhizal fungus on the root and shoot architecture of coffee-shading walnut (Cordia alliodora [Ruiz et Pav.] Oken

    Directory of Open Access Journals (Sweden)

    Rodriguez Pérez Loyla

    2012-04-01

    Full Text Available

    <em>Cordia alliodora em>(coffee-shading walnut is a species of economic importance to Colombia because of its multiple uses for farmers and for reforestation as a timber and industrial resources, mainly in the central coffee region of the country. The aim of this research was to study parameters of <em>C. alliodoraem> shoot and root system architecture over the first year of development. Plants were established in Pacho municipality (Cundinamarca at an 2,150 m a.s.l. A split plot design with a randomized complete block and three replicates was applied. Within the main plot, we evaluated the substrates (soil, husk and compost 2:1:1; soil and husk 3:2; soil and husk 3:1, and withing the subplot, the mycorrhizal fungus (without fungus, <em>Kuklospora colombianaem>, <em>Glomus manihotis em>and <em>Acaulospora> <em>lacunosa>. Substrate type affected <em>C. alliodora em>root and shoot architectural parameters. Plants transplanted into soil, husk and compost 2:1:1 had the best responses in architectural parameters: diameter of the base of the root (7.82 mm, number of secondary roots (48.4, root dry weight (5.38 g, number of leaves (47.33, dry weight of shoots (7.71 g, shoot length (72.64 g and leaf dry weight (6.28 g at 384 days after transplant. Since no fertilizer was applied to coffee-shading walnut plants, we conclude that the mycorrhizal fungi facilitated a better use and development of mineral elements present in the substrates.

  19. Depth and Diameter of the Parent Roots of Aspen Root Suckers

    Science.gov (United States)

    Robert E. Farmer

    1962-01-01

    Studies of the Populus tremuloides root system by Day (1944), Sandberg (1951) and Barnes (1959) have all shown lateral roots extending as much as 30 feet from tree base. These roots may branch extensively and sometimes exhibit an "undulating" growth habit. According to the above authors, suckers occur on the segments of these lateral roots...

  20. Root-zone temperature and water availability affect early root growth of planted longleaf pine

    Science.gov (United States)

    M.A. Sword

    1995-01-01

    Longleaf pine seedlings from three seed sources were exposed to three root-zone temperatures and three levels of water availability for 28 days. Root growth declined as temperature and water availability decreased. Root growth differed by seed source. Results suggest that subtle changes in the regeneration environment may influence early root growth of longleaf pine...

  1. OpenSimRoot: widening the scope and application of root architectural models.

    Science.gov (United States)

    Postma, Johannes A; Kuppe, Christian; Owen, Markus R; Mellor, Nathan; Griffiths, Marcus; Bennett, Malcolm J; Lynch, Jonathan P; Watt, Michelle

    2017-08-01

    OpenSimRoot is an open-source, functional-structural plant model and mathematical description of root growth and function. We describe OpenSimRoot and its functionality to broaden the benefits of root modeling to the plant science community. OpenSimRoot is an extended version of SimRoot, established to simulate root system architecture, nutrient acquisition and plant growth. OpenSimRoot has a plugin, modular infrastructure, coupling single plant and crop stands to soil nutrient and water transport models. It estimates the value of root traits for water and nutrient acquisition in environments and plant species. The flexible OpenSimRoot design allows upscaling from root anatomy to plant community to estimate the following: resource costs of developmental and anatomical traits; trait synergisms; and (interspecies) root competition. OpenSimRoot can model three-dimensional images from magnetic resonance imaging (MRI) and X-ray computed tomography (CT) of roots in soil. New modules include: soil water-dependent water uptake and xylem flow; tiller formation; evapotranspiration; simultaneous simulation of mobile solutes; mesh refinement; and root growth plasticity. OpenSimRoot integrates plant phenotypic data with environmental metadata to support experimental designs and to gain a mechanistic understanding at system scales. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

  2. Light as stress factor to plant roots - case of root halotropism.

    Science.gov (United States)

    Yokawa, Ken; Fasano, Rossella; Kagenishi, Tomoko; Baluška, František

    2014-01-01

    Despite growing underground, largely in darkness, roots emerge to be very sensitive to light. Recently, several important papers have been published which reveal that plant roots not only express all known light receptors but also that their growth, physiology and adaptive stress responses are light-sensitive. In Arabidopsis, illumination of roots speeds-up root growth via reactive oxygen species-mediated and F-actin dependent process. On the other hand, keeping Arabidopsis roots in darkness alters F-actin distribution, polar localization of PIN proteins as well as polar transport of auxin. Several signaling components activated by phytohormones are overlapping with light-related signaling cascade. We demonstrated that the sensitivity of roots to salinity is altered in the light-grown Arabidopsis roots. Particularly, light-exposed roots are less effective in their salt-avoidance behavior known as root halotropism. Here we discuss these new aspects of light-mediated root behavior from cellular, physiological and evolutionary perspectives.

  3. Avaliação in vivo da confiabilidade do localizador eletrônico bingo na determinação do comprimento de trabalho, em situações de polpa vital e necrosada = In vivo analysis of the performance of bingo apex locator to determine root length in vital and necrotic pulps

    Directory of Open Access Journals (Sweden)

    Beilke, Letícia Ponzi

    2005-01-01

    Full Text Available O objetivo deste trabalho foi avaliar in vivo, a confiabilidade do localizador eletrônico Bingo, na determinação do comprimento de trabalho, em situações de polpa vital e necrosada. Foram realizadas 56 aferições eletrônicas em pacientes entre 15 e 65 anos de idade, em tratamento na Disciplina de Endodontia da Universidade Luterana do Brasil (ULBRA – Canoas/RS. Dos dentes estudados, 25 possuíam canais com polpas vitais e 31, com polpa necrosada. Após isolamento absoluto e abertura coronária, estando a alça labial devidamente posicionada, introduziu-se, no interior do canal radicular, uma lima endodôntica de 25 ou 31 mm conectada a um dos pólos do aparelho, de diâmetro compatível com a anatomia do terço apical. Quando o visor do localizador eletrônico acusava estar a ponta do instrumento a 1mm da saída foraminal, os dentes foram radiografados. Com o auxílio de uma lupa, régua milimetrada e negatoscópio, foi mensurada, na radiografia, a distância entre a ponta do instrumento e o vértice radiográfico. Medidas compreendidas entre 0,5mm e 1,5mm foram consideradas confiáveis. Os resultados obtidos mostraram índice de confiabilidade de 92% para dentes com polpa viva e 83,87% para dentes com polpa mortificada. O localizador apical Bingo constitui-se em um excelente recurso auxiliar na odontometria, quando corretamente empregado

  4. Intersect a quartic to extract its roots

    Directory of Open Access Journals (Sweden)

    Raghavendra G. Kulkarni

    2017-12-01

    Full Text Available In this note we present a new method for determining the roots of a quartic polynomial, wherein the given quartic polynomial is intersected by a quadratic polynomial (which has two unknown coefficients at its root point; so the root satisfies both the quartic and the quadratic equations. Elimination of the root term from the two equations leads to an expression in the two unknowns of quadratic polynomial. In addition, we introduce another expression in one unknown, which leads to determination of the two unknowns and subsequently the roots of quartic polynomial.

  5. Asteroidal Quadruples in non Rooted Path Graphs

    Directory of Open Access Journals (Sweden)

    Gutierrez Marisa

    2015-11-01

    Full Text Available A directed path graph is the intersection graph of a family of directed subpaths of a directed tree. A rooted path graph is the intersection graph of a family of directed subpaths of a rooted tree. Rooted path graphs are directed path graphs. Several characterizations are known for directed path graphs: one by forbidden induced subgraphs and one by forbidden asteroids. It is an open problem to find such characterizations for rooted path graphs. For this purpose, we are studying in this paper directed path graphs that are non rooted path graphs. We prove that such graphs always contain an asteroidal quadruple.

  6. Medicolegal aspects of iatrogenic root perforations

    DEFF Research Database (Denmark)

    Tsesis, I; Rosen, E; Bjørndal, L

    2014-01-01

    AIM: To retrospectively analyze the medico-legal aspects of iatrogenic root perforations (IRP) that occurred during endodontic treatments. METHODOLOGY: A comprehensive search in a professional liability insurance database was conducted to retrospectively identify cases of IRP following root canal...... treatment (p root perforation is a complication of root canal treatment and may result in tooth extraction...... and in legal actions against the treating practitioner. Mandibular molars are more prone to medico-legal claims related to root perforations. The patient should be informed of the risks during RCT and should get information on alternative treatments and their risks and prognosis...

  7. Assessment of the wetting behavior of three different root canal sealers on root canal dentin

    OpenAIRE

    Muralidhar Tummala; Veeramachaneni Chandrasekhar; A Shashi Rashmi; M Kundabala; Vasudev Ballal

    2012-01-01

    Aim: The objective of the present study was to evaluate and compare the wetting behavior of three different root canal sealers on the root canal dentin surface treated with irrigants and their combination. Materials and Methods: Decoronation and apical third resections of 27 extracted single-rooted human mandibular premolars were done. The roots were then split longitudinally into two halves, and randomly assigned into three treatment groups (n=18). The root dentin surfaces in Group1, Gro...

  8. Etiology and epidemiology of Pythium root rot in hydroponic crops: current knowledge and perspectives

    Directory of Open Access Journals (Sweden)

    John Clifford Sutton

    2006-09-01

    knowledge and understanding of the etiology and epidemiology of root rot, and its effects on the physiology of the whole plant, are discussed in relation to new research directions and development of better practices to manage the disease in hydroponic crops. Focus is on methods and technologies for tracking Pythium and root rot, and on developing, integrating, and optimizing treatments to suppress the pathogen in the root zone and progress of root rot.A etiologia e a epidemiologia da podridão radicular causada por Pythium spp. em cultivo hidropônico são revisadas com ênfase em conhecimentos e conceitos considerados importantes para o manejo de doenças em estufas comerciais. A podridão radicular causada por Pythium continuamente ameaça a produtividade de diversas culturas em sistemas hidropônicos, incluindo pepino, tomate, pimentão, espinafre, alface, capuchinha, rúcula, rosa, e crisântemo. Os principais agentes causais incluem Pythium aphanidermatum, Pythium dissotocum, espécies de Pythium do grupo F e Pythium ultimum var. ultimum. São apresentadas e discutidas as principais fontes do inóculo primário de Pythium spp. em sistemas hidropônicos, os processos de infecção e colonização de raízes pelos patógenos, o desenvolvimento de sintomas, a produção de inóculo em raízes, e a dispersão do inóculo na solução nutritiva. Descobertas recentes da existência de um elicitor produzido especificamente por P. aphanidermatum que pode provocar a necrose (descoloração das raízes infectadas e a transição da infecção da fase biotrofica para necrotrofica são consideradas. Efeitos de fatores ligados ao hospedeiro (susceptibilidade à doença, estádio fenológico, exsudatos radiculares, compostos fenólicos, ao ambiente radicular (meio de enraizamento, concentrações de oxigênio dissolvido e compostos fenólicos na solução nutritiva, comunidades microbianas e temperatura e as interferências humanas (práticas de cultivo e medidas de controle sobre

  9. Lateral root organogenesis - from cell to organ.

    Science.gov (United States)

    Benková, Eva; Bielach, Agnieszka

    2010-12-01

    Unlike locomotive organisms capable of actively approaching essential resources, sessile plants must efficiently exploit their habitat for water and nutrients. This involves root-mediated underground interactions allowing plants to adapt to soils of diverse qualities. The root system of plants is a dynamic structure that modulates primary root growth and root branching by continuous integration of environmental inputs, such as nutrition availability, soil aeration, humidity, or salinity. Root branching is an extremely flexible means to rapidly adjust the overall surface of the root system and plants have evolved efficient control mechanisms, including, firstly initiation, when and where to start lateral root formation; secondly lateral root primordia organogenesis, during which the development of primordia can be arrested for a certain time; and thirdly lateral root emergence. Our review will focus on the most recent advances in understanding the molecular mechanisms involved in the regulation of lateral root initiation and organogenesis with the main focus on root system of the model plant Arabidopsis thaliana. Copyright © 2010 Elsevier Ltd. All rights reserved.

  10. Shaping 3D Root System Architecture.

    Science.gov (United States)

    Morris, Emily C; Griffiths, Marcus; Golebiowska, Agata; Mairhofer, Stefan; Burr-Hersey, Jasmine; Goh, Tatsuaki; von Wangenheim, Daniel; Atkinson, Brian; Sturrock, Craig J; Lynch, Jonathan P; Vissenberg, Kris; Ritz, Karl; Wells, Darren M; Mooney, Sacha J; Bennett, Malcolm J

    2017-09-11

    Plants are sessile organisms rooted in one place. The soil resources that plants require are often distributed in a highly heterogeneous pattern. To aid foraging, plants have evolved roots whose growth and development are highly responsive to soil signals. As a result, 3D root architecture is shaped by myriad environmental signals to ensure resource capture is optimised and unfavourable environments are avoided. The first signals sensed by newly germinating seeds - gravity and light - direct root growth into the soil to aid seedling establishment. Heterogeneous soil resources, such as water, nitrogen and phosphate, also act as signals that shape 3D root growth to optimise uptake. Root architecture is also modified through biotic interactions that include soil fungi and neighbouring plants. This developmental plasticity results in a 'custom-made' 3D root system that is best adapted to forage for resources in each soil environment that a plant colonises. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Optimal root arrangement of cereal crops

    Science.gov (United States)

    Jung, Yeonsu; Park, Keunhwan; Kim, Ho-Young

    2015-11-01

    The plant root absorbs water from the soil and supplies it to the rest part of the plant. It consists of a number of root fibers, through whose surfaces water uptake occurs. There is an intriguing observation that for most of cereal crops such as maize and wheat, the volume density of root in the soil declines exponentially as a function of depth. To understand this empirical finding, we construct a theoretical model of root water uptake, where mass transfer into root surface is modeled just as heat flux around a fin. Agreement between the theoretically predicted optimal root distribution in vertical direction and biological data supports the hypothesis that the plant root has evolved to achieve the optimal water uptake in competition with neighbors. This study has practical implication in the agricultural industry as well as optimal design of water transport networks in both micro- and macroscales. Department of Mechanical and Aerospace Engineering, Seoul National University, Seoul 08826, Korea.

  12. Surface Electrical Potentials of Root Cell Plasma Membranes: Implications for Ion Interactions, Rhizotoxicity, and Uptake

    Directory of Open Access Journals (Sweden)

    Yi-Min Wang

    2014-12-01

    Full Text Available Many crop plants are exposed to heavy metals and other metals that may intoxicate the crop plants themselves or consumers of the plants. The rhizotoxicity of heavy metals is influenced strongly by the root cell plasma membrane (PM surface’s electrical potential (ψ0. The usually negative ψ0 is created by negatively charged constituents of the PM. Cations in the rooting medium are attracted to the PM surface and anions are repelled. Addition of ameliorating cations (e.g., Ca2+ and Mg2+ to the rooting medium reduces the effectiveness of cationic toxicants (e.g., Cu2+ and Pb2+ and increases the effectiveness of anionic toxicants (e.g., SeO42− and H2AsO4−. Root growth responses to ions are better correlated with ion activities at PM surfaces ({IZ}0 than with activities in the bulk-phase medium ({IZ}b (IZ denotes an ion with charge Z. Therefore, electrostatic effects play a role in heavy metal toxicity that may exceed the role of site-specific competition between toxicants and ameliorants. Furthermore, ψ0 controls the transport of ions across the PM by influencing both {IZ}0 and the electrical potential difference across the PM from the outer surface to the inner surface (Em,surf. Em,surf is a component of the driving force for ion fluxes across the PM and controls ion-channel voltage gating. Incorporation of {IZ}0 and Em,surf into quantitative models for root metal toxicity and uptake improves risk assessments of toxic metals in the environment. These risk assessments will improve further with future research on the application of electrostatic theory to heavy metal phytotoxicity in natural soils and aquatic environments.

  13. Patterns of variability in the diameter of lateral roots in the banana root system.

    Science.gov (United States)

    Lecompte, François; Pagès, Loïc; Ozier-Lafontaine, Harry

    2005-09-01

    The relative importance of root system structure, plant carbon status and soil environment in the determination of lateral root diameter remains unclear, and was investigated in this study. Banana (Musa acuminata) plants were grown at various moderate levels of soil compaction in two distinct experiments, in a field experiment (FE) and in a glasshouse experiment (GE). Radiant flux density was 5 times lower in GE. The distribution of root diameter was measured for several root branching orders. Root diameters ranged between 0.09 and 0.52 mm for secondary roots and between 0.06 and 0.27 mm for tertiary roots. A relationship was found between the diameter of the parent bearing root and the median diameter of its laterals, which appears to be valid for a wide range of species. Mean lateral root diameter increased with distance to the base of the root and decreased with branching density [number of lateral roots per unit length of bearing root (cm(-1))]. Typical symptoms of low light availability were observed in GE. In this case, lateral root diameter variability was reduced. Although primary root growth was affected by soil compaction, no effects on lateral root diameter were observed.

  14. Effects of fine root length density and root biomass on soil preferential flow in forest ecosystems

    Directory of Open Access Journals (Sweden)

    Yinghu Zhang

    2015-04-01

    Full Text Available Aim of study: The study was conducted to characterize the impacts of plant roots systems (e.g., root length density and root biomass on soil preferential flow in forest ecosystems. Area of study: The study was carried out in Jiufeng National Forest Park, Beijing, China. Material and methods: The flow patterns were measured by field dye tracing experiments. Different species (Sophora japonica Linn,Platycladus orientalis Franco, Quercus dentata Thunbwere quantified in two replicates, and 12 soil depth were applied. Plant roots were sampled in the sieving methods. Root length density and root biomass were measured by WinRHIZO. Dye coverage was implied in the image analysis, and maximum depth of dye infiltration by direct measurement. Main results: Root length density and root biomass decreased with the increasing distance from soil surface, and root length density was 81.6% higher in preferential pathways than in soil matrix, and 66.7% for root biomass with respect to all experimental plots. Plant roots were densely distributed in the upper soil layers. Dye coverage was almost 100% in the upper 5-10 cm, but then decreased rapidly with soil depth. Root length density and root biomass were different from species: Platycladus orientalis Franco > Quercus dentata Thunb > Sophora japonica Linn. Research highlights: The results indicated that fine roots systems had strong effects on soil preferential flow, particularly root channels enhancing nutrition transport across soil profiles in forest dynamics.

  15. Rooting experiments with cuttings of Salix caprea

    Energy Technology Data Exchange (ETDEWEB)

    Ericsson, T.

    1981-01-01

    The ability of the following external factors on inducing root formation on leafless cuttings of Salix caprea L. was investigated: root promoting hormones, centrifugation, diffusate (plant sap), and planting substrates. The greatest number of rooted Salix caprea cuttings was obtained after treatment with 40 ppM IBA during 24 hours. However, the young root systems were very susceptile to fungal infections. Thus, the initial positive effect of the root promoting hormone was neutralized by the low survival among the test plants. Neither centrifugation nor diffusate treatment of the cuttings increased the rooting percentage further. Hormone treatment of young shoots or buds grown in water resulted in vigorous root formation on all stem sections placed below the water surface. Practically all vegetative buds on each sprout gave rise to new plants. Planting in coarse sand (3 mm) resulted in healthier plants and stronger root systems compared with cuttings rooted in peat-sand mixture (75/25 vol %), agar, and fine grained sand. Roots developed in coarse sand were spread along a longer stem section compared with roots formed in a compact substrat. Plants with a despersed root system tended to be less harmed by fungal infections than plants with all roots concentrated at the base. It is concluded that the main problem associated with vegetative propagation of Salix caprea, in the traditional manner, is the susceptibility of the hormontreated cuttings against fungal infections and not the root formations step in it self. Hormone treatment of young shoots or buds is proposed as one conceivable solution on this problem.

  16. Biophysical rhizosphere processes affecting root water uptake.

    Science.gov (United States)

    Carminati, A; Zarebanadkouki, M; Kroener, E; Ahmed, M A; Holz, M

    2016-06-26

    Recent advances in imaging techniques now make it possible to visualize the biogeochemical and physical environment around the roots, the rhizosphere. Detailed images of pore space geometry and water content dynamics around roots have demonstrated the heterogeneity of the rhizosphere compared with the soil far from the roots. These findings have inspired new models of root water uptake which aim to describe such small-scale heterogeneity. However, the question remains of how far these image-based findings have really advanced our understanding of how roots extract water from soils. The rhizosphere processes affecting root water uptake are reviewed. Special attention is dedicated to the role of mucilage exuded by roots. Mucilage increases the soil moisture at negative water potentials and it keeps the rhizosphere wet when plants take up water, possibly maintaining the hydraulic connection between roots and soil. However, mucilage becomes viscous and hydrophobic upon severe drying and it limits the water fluxes across the rhizosphere during the rewetting phase. The role of mucilage in maintaining the hydraulic contact between the root surface and the surrounding soil, thereby softening the drops in water potential around the roots in dry soils, remains to be demonstrated. Despite detailed images of water content, water fluxes and soil structure in the rhizosphere, a general understanding of how the rhizosphere affects root water uptake is still lacking. The missing elements of the puzzle are the gradient in water potential around roots. Measurements of the xylem water potential at varying soil water potentials and transpiration rates supported by numerical models of root water uptake would allow the estimation of the water potential across the rhizosphere. Such measurements are crucial to comprehend how water enters the roots. © The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions

  17. Comparative study of smear layer removal by different etching modalities and Er:YAG laser irradiation on the root surface: a scanning electron microscopy study; Estudo comparativo, atraves de microscopia eletronica de varredura, da acao de diferentes substancias quimicas e do laser de Er:YAG, na remocao de smear layer, em superficies radiculares submetidas a raspagem e aplainamento

    Energy Technology Data Exchange (ETDEWEB)

    Theodoro, Leticia Helena

    2001-07-01

    The aim of this study was to compare the effects of citric acid, EDTA, citric acid with tetracycline, and Er:YAG laser to smear layer removal on the root surface after scaling with manual instruments by SEM. Thirty specimens (n=30) of root surface before scaling were divided into 6 groups (n=5). The Control Group (G1) was not treated; Group 2 (G2) was conditioned with citric acid gel 24%, pH1, during 2 minutes; Group 3 (G3) was conditioned with EDTA gel 24%, pH 7, during 2 minutes; Group 4 (G4) was conditioned with citric acid and tetracycline gel 50%, pH1 during 2 minutes; Group 5 (G5) was irradiated with Er:YAG laser (2.94 {mu}m), 47 mJ/10 Hz, focused, under water spray during 15 seconds and fluence of 0.58 J/cm{sup 2}; Group 6 (G6) was irradiated with Er:YAG laser (2.94{mu}m), 83 mJ/10 Hz, focused, under water spray during 15 seconds and fluence of 1.03 J/cm{sup 2}. The micrographic were analyzed by scores and following the statistical analysis with Kruskal Wallis (p<0.05) H=20,31. The G1 was significantly different of all groups (28.0); the G2 (13.4), G3 (11.7), and G4 (13.6) showed no difference in relation to G5 (20.3) and G6 (6.0), but the G6 was significantly different from G5. From the results, it can be conclude that: 1) there was intensity smear layer after scaling and root planing; 2) all treatments were effective to smear layer remove with significantly difference to G2, G3, G4, G5 and G6; G2, G3 and G4 were not statistically different from G5 and G6; 3) G6 was more effective in the smear layer remotion in relation to G5 and both presented irregular root surface. (author)

  18. EM International. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    1993-07-01

    It is the intent of EM International to describe the Office of Environmental Restoration and Waste Management`s (EM`s) various roles and responsibilities within the international community. Cooperative agreements and programs, descriptions of projects and technologies, and synopses of visits to international sites are all highlighted in this semiannual journal. Focus on EM programs in this issue is on international collaboration in vitrification projects. Technology highlights covers: in situ sealing for contaminated sites; and remote sensors for toxic pollutants. Section on profiles of countries includes: Arctic contamination by the former Soviet Union, and EM activities with Germany--cooperative arrangements.

  19. relative performance of root and shoot development in enset and ...

    African Journals Online (AJOL)

    jen

    diameter at the base of the cord roots (AD) and root dry weight (RW). Cord root length was measured using the line intersect method. (Tennant, 1975), while the diameter of the cord roots was measured with a Vernier Caliper. The shoot-root ratio was calculated as the ratio of leaf, pseudostem and corm dry weight over root.

  20. Changes of Root Length and Root-to-Crown Ratio after Apical Surgery

    DEFF Research Database (Denmark)

    von Arx, Thomas; Jensen, Simon S; Bornstein, Michael M

    2015-01-01

    INTRODUCTION: Apical surgery is an important treatment option for teeth with post-treatment periodontitis. Although apical surgery involves root-end resection, no morphometric data are yet available about root-end resection and its impact on the root-to-crown ratio (RCR). The present study assessed...... the length of apicectomy and calculated the loss of root length and changes of RCR after apical surgery. METHODS: In a prospective clinical study, cone-beam computed tomography scans were taken preoperatively and postoperatively. From these images, the crown and root lengths of 61 roots (54 teeth in 47....... The following parameters were assessed for all treated teeth as well as for specific tooth groups: length of root-end resection and percentage change of root length, preoperative and postoperative RCRs, and percentage change of RCR after apical surgery. RESULTS: The mean length of root-end resection was 3...

  1. Assessment of the nonoperated root after apical surgery of the other root in mandibular molars

    DEFF Research Database (Denmark)

    Kraus, Riccardo D; von Arx, Thomas; Gfeller, David

    2015-01-01

    INTRODUCTION: If a surgical approach is chosen to treat a multirooted tooth affected by persistent periapical pathosis, usually only the affected roots are operated on. The present study assessed the periapical status of the nonoperated root 5 years after apical surgery of the other root...... and radiographs 5 years after surgery were examined. The following data were collected: tooth, operated root, type and quality of the coronal restoration, marginal bone level, length and homogeneity of the root canal filling, presence of a post/screw, periapical index (PAI) of each root, and radiographic healing...... of the operated root. The presence of apical pathosis of the nonoperated root was analyzed statistically in relation to the recorded variables. RESULTS: Thirty-seven patients fulfilled the inclusion criteria. Signs of periapical pathosis in the nonoperated root 5 years after surgery (PAI ≥ 3) could be observed...

  2. Inhibition of auxin movement from the shoot into the root inhibits lateral root development in Arabidopsis

    Science.gov (United States)

    Reed, R. C.; Brady, S. R.; Muday, G. K.

    1998-01-01

    In roots two distinct polar movements of auxin have been reported that may control different developmental and growth events. To test the hypothesis that auxin derived from the shoot and transported toward the root controls lateral root development, the two polarities of auxin transport were uncoupled in Arabidopsis. Local application of the auxin-transport inhibitor naphthylphthalamic acid (NPA) at the root-shoot junction decreased the number and density of lateral roots and reduced the free indoleacetic acid (IAA) levels in the root and [3H]IAA transport into the root. Application of NPA to the basal half of or at several positions along the root only reduced lateral root density in regions that were in contact with NPA or in regions apical to the site of application. Lateral root development was restored by application of IAA apical to NPA application. Lateral root development in Arabidopsis roots was also inhibited by excision of the shoot or dark growth and this inhibition was reversible by IAA. Together, these results are consistent with auxin transport from the shoot into the root controlling lateral root development.

  3. A new method to optimize root order classification based on the diameter interval of fine root.

    Science.gov (United States)

    Liu, Ying; Wang, Guoliang; Yu, Kunxia; Li, Peng; Xiao, Lie; Liu, Guobin

    2018-02-13

    Plant roots are a highly heterogeneous and hierarchical system. Although the root-order method is superior to the root diameter method for revealing differences in the morphology and physiology of fine roots, its complex partitioning limits its application. Whether root order can be determined by partitioning the main root based on its diameter remains uncertain. Four methods were employed for studying the morphological characteristics of seedling roots of two Pinus species in a natural and nitrogen-enriched environment. The intrinsic relationships among categories of roots by root order and diameter were systematically compared to explore the possibility of using the latter to describe root morphology. The normal transformation method proved superior to the other three in that the diameter intervals corresponded most closely (at least 68.3%) to the morphological characteristics. The applied methods clearly distinguished the results from the natural and nitrogen-rich environments. Considering both root diameter and order simplified the classification of fine roots, and improved the estimation of root lifespan and the data integrity of field collection, but failed to partition all roots into uniform diameter intervals.

  4. Enraizamento de estacas de Sebastiania schottiana Müll. Arg. Rooting of Sebastiania schottiana Müll. Arg. cuttings

    Directory of Open Access Journals (Sweden)

    Eduardo Garcia Frassetto

    2010-12-01

    Full Text Available O pouco domínio dos processos envolvidos no enraizamento de estacas de diversas espécies florestais tem sido um fator limitador para a produção de mudas via propagação vegetativa, impedindo possíveis ganhos econômicos e ecológicos nas atividades de reflorestamento e recuperação de áreas degradadas. O objetivo deste estudo foi verificar o potencial de enraizamento de estacas de Sebastiania schottiana, submetidas a diferentes doses e tempos de imersão em produto comercial Orgasol® FTS (composto de aminoácidos e micronutrientes. Estacas basais, medianas e apicais tiveram suas bases imersas em Orgasol® FTS nas doses de 0; 5; 10 e 15mL L-1, durante 1 minuto. O delineamento experimental foi realizado em blocos ao acaso, com três repetições de 20 estacas por parcela. Aos 75 dias, avaliou-se a porcentagem de estacas enraizadas, o comprimento e diâmetro da raiz mais longa. Em um segundo experimento, estacas basais e medianas foram tratadas em 15mL L-1 do produto, durante 0, 15, 30 e 45 minutos. O delineamento experimental foi realizado em blocos ao acaso, com três repetições e 20 estacas por parcela. Aos 150 dias, avaliou-se a porcentagem de estacas enraizadas, massa seca das raízes e o comprimento e diâmetro da raiz mais longa. No primeiro experimento, o aumento da dose do produto promoveu um maior potencial de enraizamento em todos os tipos de estacas, com maior porcentagem de estacas enraizadas no tratamento com 15mL L-1 do produto. No segundo experimento, os maiores potenciais de enraizamento foram observados em estacas basais e medianas tratadas com 15mL L-1 do produto, por 45 minutos. O uso do produto comercial Orgasol® FTS favoreceu a indução de raízes adventícias em estacas de Sebastiania schottiana.The aim of this study was to verify the rooting potential of Sebastiania schottiana cuttings treated with different doses and immersion times in Orgasol® FTS (composed with amino acids and micronutrients. In a first

  5. Mitochondrial cytochrome <em>B> sequence divergence among Spanish, Alpine and Abruzzo chamois (genus <em>Rupicapra>

    Directory of Open Access Journals (Sweden)

    Nadia Mucci

    1998-12-01

    Full Text Available Abstract We have studied genetic divergence and phylogenetic relationships of Alpine, Spanish and Abruzzo chamois (genus <em>Rupicapra> by sequencing a region of 330 nucleotides within the mitochondrial DNA cytochrome <em>b> gene (mtDNA cyt <em>b>. These sequences were aligned with additional homologous sequences of Caprinae: Japanese serow, Chinese goral, Canadian mountain goat, Mishmi takin, muskox, Sardinian mouflon and domestic goat. Results suggest that, using representatives of the Bovini as outgroups, the Caprinae constitute a monophyletic clade. However, inferred phylogenetic relationships among and within tribes of Caprinae were poorly defined and did not reflect current evolutionary and taxonomical views. In fact, the Asian Rupicaprini goral and serow constituted a strongly supported clade, which included the muskox, while the takin grouped with <em>Ovis>. Therefore, the monophyly of Ovibovini was not supported by cyt <em>b> sequences. Species of <em>Rupicapra> joined a strongly supported monophyletic clade, which was distantly related to the Asian rupicaprins and <em>Oreamnos>. Therefore, the monophyly of the Rupicaprini was not supported by these cyt <em>b> sequences. There were sister species relationships within <em>Rupicapra>, Spanish and Alpine chamois and the Abruzzo chamois (<em>Rupicapra pyrenaica ornataem> was strictly related to the Spanish chamois (<em>Rupicapra pyrenaica parvaem>, as previously suggested by allozyme data and biogeographic reconstructions.

  6. New Trifluoromethyl Triazolopyrimidines as Anti-<em>Plasmodium> <em>falciparum> Agents

    Directory of Open Access Journals (Sweden)

    Núbia Boechat

    2012-07-01

    Full Text Available According to the World Health Organization, half of the World’s population, approximately 3.3 billion people, is at risk for developing malaria. Nearly 700,000 deaths each year are associated with the disease. Control of the disease in humans still relies on chemotherapy. Drug resistance is a limiting factor, and the search for new drugs is important. We have designed and synthesized new 2-(trifluoromethyl[1,2,4]triazolo[1,5-<em>a>]pyrimidine derivatives based on bioisosteric replacement of functional groups on the anti-malarial compounds mefloquine and amodiaquine. This approach enabled us to investigate the impact of: (i ring bioisosteric replacement; (ii a CF3 group substituted at the 2-position of the [1,2,4]triazolo[1,5-<em>a>]pyrimidine scaffold and (iii a range of amines as substituents at the 7-position of the of heterocyclic ring; on <em>in vitroem> activity against <em>Plasmodium falciparumem>. According to docking simulations, the synthesized compounds are able to interact with <em>P. falciparumem> dihydroorotate dehydrogenase (<em>Pf>DHODH through strong hydrogen bonds. The presence of a trifluoromethyl group at the 2-position of the [1,2,4]triazolo[1,5-<em>a>]pyrimidine ring led to increased drug activity. Thirteen compounds were found to be active, with IC50 values ranging from 0.023 to 20 µM in the anti-HRP2 and hypoxanthine assays. The selectivity index (SI of the most active derivatives 5, 8, 11 and 16 was found to vary from 1,003 to 18,478.

  7. Roots at the percolation threshold.

    Science.gov (United States)

    Kroener, Eva; Ahmed, Mutez Ali; Carminati, Andrea

    2015-04-01

    The rhizosphere is the layer of soil around the roots where complex and dynamic interactions between plants and soil affect the capacity of plants to take up water. The physical properties of the rhizosphere are affected by mucilage, a gel exuded by roots. Mucilage can absorb large volumes of water, but it becomes hydrophobic after drying. We use a percolation model to describe the rewetting of dry rhizosphere. We find that at a critical mucilage concentration the rhizosphere becomes impermeable. The critical mucilage concentration depends on the radius of the soil particle size. Capillary rise experiments with neutron radiography prove that for concentrations below the critical mucilage concentration water could easily cross the rhizosphere, while above the critical concentration water could no longer percolate through it. Our studies, together with former observations of water dynamics in the rhizosphere, suggest that the rhizosphere is near the percolation threshold, where small variations in mucilage concentration sensitively alter the soil hydraulic conductivity. Is mucilage exudation a plant mechanism to efficiently control the rhizosphere conductivity and the access to water?

  8. A Rooted Net of Life

    Directory of Open Access Journals (Sweden)

    Green Anna G

    2011-09-01

    Full Text Available Abstract Phylogenetic reconstruction using DNA and protein sequences has allowed the reconstruction of evolutionary histories encompassing all life. We present and discuss a means to incorporate much of this rich narrative into a single model that acknowledges the discrete evolutionary units that constitute the organism. Briefly, this Rooted Net of Life genome phylogeny is constructed around an initial, well resolved and rooted tree scaffold inferred from a supermatrix of combined ribosomal genes. Extant sampled ribosomes form the leaves of the tree scaffold. These leaves, but not necessarily the deeper parts of the scaffold, can be considered to represent a genome or pan-genome, and to be associated with members of other gene families within that sequenced (pangenome. Unrooted phylogenies of gene families containing four or more members are reconstructed and superimposed over the scaffold. Initially, reticulations are formed where incongruities between topologies exist. Given sufficient evidence, edges may then be differentiated as those representing vertical lines of inheritance within lineages and those representing horizontal genetic transfers or endosymbioses between lineages. Reviewers W. Ford Doolittle, Eric Bapteste and Robert Beiko.

  9. Sintomatologia da murcha de Ceratocystis fimbriata em eucalipto Symptomatology of Ceratocystis wilt in eucalyptus

    Directory of Open Access Journals (Sweden)

    Francisco Alves Ferreira

    2006-04-01

    Full Text Available Descreveram-se o histórico da murcha de Ceratocystis fimbriata em eucalipto no Brasil e em outros países e a sintomatologia da doença, em plantações clonais com 4 meses a 5 anos de idade, em brotações de tocos, em estacas em enraizamento e em mudas clonais em viveiro, de quatro estados brasileiros. O patógeno evoluía-se da extremidade da raiz, atingindo o colo e tronco acima via parênquima medular, de onde, em diversas alturas, surgiam estrias escuras, que progrediam, via parênquima radial, matando uma porção de câmbio vascular, de floema e de feloderme. Dessa progressão sistêmica do patógeno, ascendente e radialmente, resultava uma lesão longitudinal externamente no tronco, contínua ou descontínua, marrom-avermelhada, coriácea, que passava a sulcada e, posteriormente, a cancro longitudinal, com seus calos longilíneos nas duas laterais. Por esse contexto sintomatológico, pode-se considerar essa enfermidade como um modelo de doença sistêmica em essência florestal, pelo menos na subárea da patologia florestal brasileira. Em brotações novas, em estacas em enraizamento e em mudas clonais as lesões eram longitudinais, contínuas ou descontínuas, negras a arroxeadas. A inativação de xilema em raízes, colo e em diferentes alturas do tronco, ou galho, dava-se pelo adensamento das estrias radiais escuras no lenho.The symptomatology of Ceratocystis fimbriata wilt in eucalyptus was described based on observations of 4-month to 5-year-old clonal plantations, in stump sprouts, rooting cuttings, and rooted seedlings in nurseries. The disease was characterized as a model of systemic disease in woody plants, which starts in roots and progresses upward to the collar and trunk through the medullar parenchyma, where dark stripes irradiate and lead a portion of the vascular cambium, phloem and phelloderm to death. As the pathogen spread upward and radially, a longitudinal reddish-brown, continuous or non-continuous corky lesion

  10. Tree-root control of shallow landslides

    Directory of Open Access Journals (Sweden)

    D. Cohen

    2017-08-01

    Full Text Available Tree roots have long been recognized to increase slope stability by reinforcing the strength of soils. Slope stability models usually include the effects of roots by adding an apparent cohesion to the soil to simulate root strength. No model includes the combined effects of root distribution heterogeneity, stress-strain behavior of root reinforcement, or root strength in compression. Recent field observations, however, indicate that shallow landslide triggering mechanisms are characterized by differential deformation that indicates localized activation of zones in tension, compression, and shear in the soil. Here we describe a new model for slope stability that specifically considers these effects. The model is a strain-step discrete element model that reproduces the self-organized redistribution of forces on a slope during rainfall-triggered shallow landslides. We use a conceptual sigmoidal-shaped hillslope with a clearing in its center to explore the effects of tree size, spacing, weak zones, maximum root-size diameter, and different root strength configurations. Simulation results indicate that tree roots can stabilize slopes that would otherwise fail without them and, in general, higher root density with higher root reinforcement results in a more stable slope. The variation in root stiffness with diameter can, in some cases, invert this relationship. Root tension provides more resistance to failure than root compression but roots with both tension and compression offer the best resistance to failure. Lateral (slope-parallel tension can be important in cases when the magnitude of this force is comparable to the slope-perpendicular tensile force. In this case, lateral forces can bring to failure tree-covered areas with high root reinforcement. Slope failure occurs when downslope soil compression reaches the soil maximum strength. When this occurs depends on the amount of root tension upslope in both the slope-perpendicular and slope

  11. Thermotropism by primary roots of maize

    Energy Technology Data Exchange (ETDEWEB)

    Fortin, M.-C.; Poff, K.L. (MSU-DOE Plant Research Laboratory, East Lansing, MI (USA))

    1990-05-01

    Sensing in the roots of higher plants has long been recognized to be restricted mainly to gravitropism and thigmotropism. However, root responses to temperature gradients have not been extensively studied. We have designed experiments under controlled conditions to test if and how root direction of maize can be altered by thermal gradients perpendicular to the gravity vector. Primary roots of maize grown on agar plates exhibit positive thermotropism (curvature toward the warmer temperature) when exposed to gradients of 0.5 to 4.2{degree}C cm{sup {minus}1}. The extent of thermotropism depends on the temperature gradient and the temperature at which the root is placed within the gradient. The curvature cannot be accounted for by differential growth as a direct effect of temperature on each side of the root.

  12. Tree-root control of shallow landslides

    Science.gov (United States)

    Cohen, Denis; Schwarz, Massimiliano

    2017-08-01

    Tree roots have long been recognized to increase slope stability by reinforcing the strength of soils. Slope stability models usually include the effects of roots by adding an apparent cohesion to the soil to simulate root strength. No model includes the combined effects of root distribution heterogeneity, stress-strain behavior of root reinforcement, or root strength in compression. Recent field observations, however, indicate that shallow landslide triggering mechanisms are characterized by differential deformation that indicates localized activation of zones in tension, compression, and shear in the soil. Here we describe a new model for slope stability that specifically considers these effects. The model is a strain-step discrete element model that reproduces the self-organized redistribution of forces on a slope during rainfall-triggered shallow landslides. We use a conceptual sigmoidal-shaped hillslope with a clearing in its center to explore the effects of tree size, spacing, weak zones, maximum root-size diameter, and different root strength configurations. Simulation results indicate that tree roots can stabilize slopes that would otherwise fail without them and, in general, higher root density with higher root reinforcement results in a more stable slope. The variation in root stiffness with diameter can, in some cases, invert this relationship. Root tension provides more resistance to failure than root compression but roots with both tension and compression offer the best resistance to failure. Lateral (slope-parallel) tension can be important in cases when the magnitude of this force is comparable to the slope-perpendicular tensile force. In this case, lateral forces can bring to failure tree-covered areas with high root reinforcement. Slope failure occurs when downslope soil compression reaches the soil maximum strength. When this occurs depends on the amount of root tension upslope in both the slope-perpendicular and slope-parallel directions. Roots

  13. Unit roots, nonlinearities and structural breaks

    DEFF Research Database (Denmark)

    Haldrup, Niels; Kruse, Robinson; Teräsvirta, Timo

    One of the most influential research fields in econometrics over the past decades concerns unit root testing in economic time series. In macro-economics much of the interest in the area originate from the fact that when unit roots are present, then shocks to the time series processes have...... a selective review of contributions to the field of unit root testing over the past three decades. We discuss the nature of stochastic and deterministic trend processes, including break processes, that are likely to affect unit root inference. A range of the most popular unit root tests are presented...... and their modifications to situations with breaks are discussed. We also review some results on unit root testing within the framework of non-linear processes....

  14. Systems approaches to study root architecture dynamics

    Directory of Open Access Journals (Sweden)

    Candela eCuesta

    2013-12-01

    Full Text Available The plant root system is essential for providing anchorage to the soil, supplying minerals and water, and synthesizing metabolites. It is a dynamic organ modulated by external cues such as environmental signals, water and nutrients availability, salinity and others. Lateral roots are initiated from the primary root post-embryonically, after which they progress through discrete developmental stages which can be independently controlled, providing a high level of plasticity during root system formation.Within this review, main contributions are presented, from the classical forward genetic screens to the more recent high-throughput approaches, combined with computer model predictions, dissecting how lateral roots and thereby root system architecture is established and developed.

  15. INDUSTRIAL ENGINEERING : ROOTING FOR ROOTS, HANKERING FOR HEROES

    Directory of Open Access Journals (Sweden)

    P.S. Kruger

    2012-01-01

    Full Text Available

    ENGLISH ABSTRACT: The “roots” of Industrial Engineering are certainly extensive, diverse and deep. Similarly, there are numerous historical “heroes” that made significant contributions to the development of the Industrial Engineering discipline. For the sake of argument, this article will assume that Industrial Engineering has at least two identifiable main roots, namely Determinism and Stochastism. The article attempts to trace the early history1 of the stochastic root which is very closely linked to the history of probability and statistics and hence games of chance, gambling and divinity. Therefore, the life and times, contributions and personalities of some of the heroes and villains, champions and sad cases of the stochastic world, will be briefly discussed in a somewhat light-hearted, but not necessarily flippant, manner.

    AFRIKAANSE OPSOMMING: Die “wortel en tak” van Bedryfsingenieurswese is sekerlik van groot omvang, van diverse aard en diep gesetel. Verskeie historiese “helde” het betekenisvolle bydraes gemaak tot die ontwikkeling van die Bedryfsingenieurswesevakgebied. Ter wille van betoogvoering sal in hierdie artikel aanvaar word dat Bedryfsingenieurswese uit minstens twee identifiseerbare sub-vakgebiede bestaan naamlik : Die Determinisme en die Stogasme. ’n Poging word aangewend om die vroeë geskiedenis van die stogasme na te speur wat op sy beurt aaneengesnoer is met die geskiedenis van die waarskynlikheidsleer en statistiek en dus toevalspelle, dobbelary en wiggelary. Die lewenswyse, tydsgewrig, bydraes en persoonlikheidseienskappe van ’n aantal helde en skurke, kampioene en prulle van die stogastiese wêreld word kortliks bespreek, op ’n ietwat lighartige maar nie noodwendig ligsinnige wyse.

  16. Shoot and root responses of Trifolium vesiculosum to boron fertilization in an acidic Brazilian soil

    Directory of Open Access Journals (Sweden)

    Nerilde Favaretto

    2007-07-01

    Full Text Available To analyze the influence of boron fertilization on shoot and root growth of Trifolium vesiculosum (arrowleaf clover, an acid soil profile (60 cm depth with 67% Al saturation was recreated in a column (three layers of 20 cm each. Lime and fertilizer (P and K were incorporated into the top 20 cm. The treatments consisted of six boron rates where boric acid was mixed throughout the profile. Addition of boron to soil with low pH and high Al increased the root and shoot growth, independent of the rate applied. Boron inhibited Al toxicity, but no effect was observed in the root length when Al was not present in the soil. It was also observed that there was more root growth below the plow layer (0-20 cm, suggesting better root distribution in the soil profile which could be important for the plant growth, especially under drought conditions.Estudos têm mostrado que o boro (B afeta o crescimento das raízes em solo ácido reduzindo a toxidez do alumínio (Al. Para analisar a influência do boro no crescimento da parte aérea e raízes do Trifolium Vesiculosum (trevo vesiculoso um perfil de solo ácido (60 cm de profundidade com 67% de saturação de Al foi recriado em uma coluna (três camadas com 20 cm cada. Calcário e adubos (P e K foram incorporados na camada de 0-20 cm. Os tratamentos consistiram de seis doses de boro sendo o ácido bórico incorporado em todo o perfil. A adubação com boro em solo com baixo pH e elevado Al aumentou o crescimento da parte aérea e raízes, no entanto, independente da dose aplicada. Boro pode inibir a toxidez de Al, porém não observou-se efeito no comprimento de raízes sem a presença de Al no solo. Observou-se também um grande aumento no crescimento de raízes abaixo da camada arável (0-20 cm, fornecendo uma melhor distribuição de raízes no perfil do solo, o que pode ser importante para o crescimento da planta especialmente em condição de seca.

  17. Maximum Bounded Rooted-Tree Packing Problem

    OpenAIRE

    Kerivin, Herve; Leblet, Jimmy; Simon, Gwendal; Zhou, Fen

    2011-01-01

    Given a graph and a root, the Maximum Bounded Rooted-Tree Packing (MBRTP) problem aims at finding K rooted-trees that span the largest subset of vertices, when each vertex has a limited outdegree. This problem is motivated by peer-to-peer streaming overlays in under-provisioned systems. We prove that the MBRTP problem is NP-complete. We present two polynomial-time algorithms that computes an optimal solution on complete graphs and trees respectively.

  18. Hydrologic regulation of plant rooting depth

    Science.gov (United States)

    Fan, Ying; Miguez-Macho, Gonzalo; Jobbágy, Esteban G.; Jackson, Robert B.; Otero-Casal, Carlos

    2017-10-01

    Plant rooting depth affects ecosystem resilience to environmental stress such as drought. Deep roots connect deep soil/groundwater to the atmosphere, thus influencing the hydrologic cycle and climate. Deep roots enhance bedrock weathering, thus regulating the long-term carbon cycle. However, we know little about how deep roots go and why. Here, we present a global synthesis of 2,200 root observations of >1,000 species along biotic (life form, genus) and abiotic (precipitation, soil, drainage) gradients. Results reveal strong sensitivities of rooting depth to local soil water profiles determined by precipitation infiltration depth from the top (reflecting climate and soil), and groundwater table depth from below (reflecting topography-driven land drainage). In well-drained uplands, rooting depth follows infiltration depth; in waterlogged lowlands, roots stay shallow, avoiding oxygen stress below the water table; in between, high productivity and drought can send roots many meters down to the groundwater capillary fringe. This framework explains the contrasting rooting depths observed under the same climate for the same species but at distinct topographic positions. We assess the global significance of these hydrologic mechanisms by estimating root water-uptake depths using an inverse model, based on observed productivity and atmosphere, at 30″ (˜1-km) global grids to capture the topography critical to soil hydrology. The resulting patterns of plant rooting depth bear a strong topographic and hydrologic signature at landscape to global scales. They underscore a fundamental plant-water feedback pathway that may be critical to understanding plant-mediated global change.

  19. Root phenology in a changing climate.

    Science.gov (United States)

    Radville, Laura; McCormack, M Luke; Post, Eric; Eissenstat, David M

    2016-06-01

    Plant phenology is one of the strongest indicators of ecological responses to climate change, and altered phenology can have pronounced effects on net primary production, species composition in local communities, greenhouse gas fluxes, and ecosystem processes. Although many studies have shown that aboveground plant phenology advances with warmer temperatures, demonstration of a comparable association for belowground phenology has been lacking because the factors that influence root phenology are poorly understood. Because roots can constitute a large fraction of plant biomass, and root phenology may not respond to warming in the same way as shoots, this represents an important knowledge gap in our understanding of how climate change will influence phenology and plant performance. We review studies of root phenology and provide suggestions to direct future research. Only 29% of examined studies approached root phenology quantitatively, strongly limiting interpretation of results across studies. Therefore, we suggest that researchers emphasize quantitative analyses in future phenological studies. We suggest that root initiation, peak growth, and root cessation may be under different controls. Root initiation and cessation may be more constrained by soil temperature and the timing of carbon availability, whereas the timing of peak root growth may represent trade-offs among competing plant sinks. Roots probably do not experience winter dormancy in the same way as shoots: 89% of the studies that examined winter phenology found evidence of growth during winter months. More research is needed to observe root phenology, and future studies should be careful to capture winter and early season phenology. This should be done quantitatively, with direct observations of root growth utilizing rhizotrons or minirhizotrons. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email

  20. Hydrologic regulation of plant rooting depth.

    Science.gov (United States)

    Fan, Ying; Miguez-Macho, Gonzalo; Jobbágy, Esteban G; Jackson, Robert B; Otero-Casal, Carlos

    2017-10-03

    Plant rooting depth affects ecosystem resilience to environmental stress such as drought. Deep roots connect deep soil/groundwater to the atmosphere, thus influencing the hydrologic cycle and climate. Deep roots enhance bedrock weathering, thus regulating the long-term carbon cycle. However, we know little about how deep roots go and why. Here, we present a global synthesis of 2,200 root observations of >1,000 species along biotic (life form, genus) and abiotic (precipitation, soil, drainage) gradients. Results reveal strong sensitivities of rooting depth to local soil water profiles determined by precipitation infiltration depth from the top (reflecting climate and soil), and groundwater table depth from below (reflecting topography-driven land drainage). In well-drained uplands, rooting depth follows infiltration depth; in waterlogged lowlands, roots stay shallow, avoiding oxygen stress below the water table; in between, high productivity and drought can send roots many meters down to the groundwater capillary fringe. This framework explains the contrasting rooting depths observed under the same climate for the same species but at distinct topographic positions. We assess the global significance of these hydrologic mechanisms by estimating root water-uptake depths using an inverse model, based on observed productivity and atmosphere, at 30″ (∼1-km) global grids to capture the topography critical to soil hydrology. The resulting patterns of plant rooting depth bear a strong topographic and hydrologic signature at landscape to global scales. They underscore a fundamental plant-water feedback pathway that may be critical to understanding plant-mediated global change.

  1. Quality of root canal fillings performed by undergraduate dental students on single-rooted teeth.

    Science.gov (United States)

    Lynch, C D; Burke, F M

    2006-05-01

    Root canal therapy is an accepted and successful form of tooth conservation. Educational guidelines require dental schools to ensure that their graduates are competent on graduation at performing root canal therapy. The aim of this investigation was to assess the technical quality of root canal fillings placed by undergraduate students in single-rooted teeth. A total of 100 radiographs of root canal fillings placed by undergraduate students in single-rooted teeth were examined under even illumination in a darkened room using x2 magnification. These were graded as 'adequate', where the root canal filling was within 2 mm of the radiographic apex, 'under-filled', where the root canal filling was >2 mm from the radiographic apex, and 'over-filled', where the root canal filling was extruded beyond the radiographic apex. The presence of voids, fractured instruments, and root perforations were also noted. All teeth were obturated with gutta-percha and sealer (Roth Cement), using a cold lateral condensation technique. Of 100 teeth, 10% (n = 10) had voids. Of the remainder, 70% (n = 63) were judged to be 'acceptable', 21% (n = 19) were 'under-filled', and 9% (n = 8) were 'over-filled'. There was no evidence of fractured instruments or root perforations in any root filling examined. The quality of root canal fillings placed in single-rooted teeth by undergraduate dental students at the University Dental School and Hospital, Cork was acceptable (63% of root fillings placed in single rooted teeth were graded as 'adequate'). The probable reasons for this are multi-factorial, but may be linked to the amount of pre-clinical and clinical teaching in endodontics at the University Dental School and Hospital, Cork. It should be remembered that factors other than radiographic quality/evidence must be considered when determining the outcome of root canal therapy.

  2. Abscisic Acid Regulates Auxin Homeostasis in Rice Root Tips to Promote Root Hair Elongation

    Directory of Open Access Journals (Sweden)

    Tao Wang

    2017-06-01

    Full Text Available Abscisic acid (ABA plays an essential role in root hair elongation in plants, but the regulatory mechanism remains to be elucidated. In this study, we found that exogenous ABA can promote rice root hair elongation. Transgenic rice overexpressing SAPK10 (Stress/ABA-activated protein kinase 10 had longer root hairs; rice plants overexpressing OsABIL2 (OsABI-Like 2 had attenuated ABA signaling and shorter root hairs, suggesting that the effect of ABA on root hair elongation depends on the conserved PYR/PP2C/SnRK2 ABA signaling module. Treatment of the DR5-GUS and OsPIN-GUS lines with ABA and an auxin efflux inhibitor showed that ABA-induced root hair elongation depends on polar auxin transport. To examine the transcriptional response to ABA, we divided rice root tips into three regions: short root hair, long root hair and root tip zones; and conducted RNA-seq analysis with or without ABA treatment. Examination of genes involved in auxin transport, biosynthesis and metabolism indicated that ABA promotes auxin biosynthesis and polar auxin transport in the root tip, which may lead to auxin accumulation in the long root hair zone. Our findings shed light on how ABA regulates root hair elongation through crosstalk with auxin biosynthesis and transport to orchestrate plant development.

  3. Measurements of water uptake of maize roots: the key function of lateral roots

    Science.gov (United States)

    Ahmed, M. A.; Zarebanadkouki, M.; Kroener, E.; Kaestner, A.; Carminati, A.

    2014-12-01

    Maize (Zea mays L.) is one of the most important crop worldwide. Despite its importance, there is limited information on the function of different root segments and root types of maize in extracting water from soils. Therefore, the aim of this study was to investigate locations of root water uptake in maize. We used neutron radiography to: 1) image the spatial distribution of maize roots in soil and 2) trace the transport of injected deuterated water (D2O) in soil and roots. Maizes were grown in aluminum containers (40×38×1 cm) filled with a sandy soil. When the plants were 16 days old, we injected D2O into selected soil regions containing primary, seminal and lateral roots. The experiments were performed during the day (transpiring plants) and night (not transpiring plants). The transport of D2O into roots was simulated using a new convection-diffusion numerical model of D2O transport into roots. By fitting the observed D2O transport we quantified the diffusional permeability and the water uptake of the different root segments. The maize root architecture consisted of a primary root, 4-5 seminal roots and many lateral roots connected to the primary and seminal roots. Laterals emerged from the proximal 15 cm of the primary and seminal roots. Water uptake occurred primarily in lateral roots. Lateral roots had the highest diffusional permeability (9.4×10-7), which was around six times higher that the diffusional permeability of the old seminal segments (1.4×10-7), and two times higher than the diffusional permeability of the young seminal segments (4.7×10-7). The radial flow of D2O into the lateral (6.7×10-5 ) was much higher than in the young seminal roots (1.1×10-12). The radial flow of D2O into the old seminal was negligible. We concluded that the function of the primary and seminal roots was to collect water from the lateral roots and transport it to the shoot. A maize root system with lateral roots branching from deep primary and seminal roots would be

  4. Study of root and leaf rachis of Spathelia excelsa: phytochemistry and activity against fungus Moniliophthora perniciosa associated with cupuassu (Theobroma grandiflorum); Estudo em raiz e raquis foliar de Spathelia excelsa: fitoquimica e atividade frente ao fungo Moniliophthora perniciosa associado ao cupuacuzeiro (Theobroma grandiflorum)

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Loretta Ennes de; Lima, Maria da Paz; Maximo, Ariane da Costa; Pereira, Elaine Cristina da Silva; Moreira, Wagner Alan dos Santos [Instituto Nacional de Pesquisas da Amazonia (INPA), Manaus, AM (Brazil). Coordenacao de Pesquisas em Tecnologia e Inovacao; Ferreira, Antonio Gilberto [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica; Veras, Solange de Mello [Universidade Federal do Amazonas (UFAM), Manaus, AM (Brazil). Fac. de Ciencias Agrarias; Souza, Maria Geralda de, E-mail: mdapaz@inpa.gov.br [Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), Manaus, AM (Brazil)

    2012-07-01

    The chemical composition of Spathelia excelsa (Krause) R. S. Cowan and Brizicky was investigated and the limonoids harrisonin (1) and deacetylspathelin (2), alkaloids folinin and casimiroin mixture (3a,b), plus a further casimiroin (3b) were identified in methanol extract from root. The CH{sub 2}Cl{sub 2} extract from the rachis yielded protolimonoid 3{beta}-angeloyl-21,24-epoxy-7{alpha},21{alpha},23{alpha},25-tetrahydroxy-4{alpha},4{beta},8{beta},10{beta}-tetramethyl -25-dimethyl-14,18-cyclo-5{alpha},13{alpha},14{alpha},17{alpha}-cholestane (4), and methanol extract, the limonoids limonin diosphenol (5) and perforatin (6), as well as the chromone biflorin (7). Harrisonin and biflorin were isolated for the first time in this genus. On the antifungal assay against witches' broom (Moniliophthora perniciosa) compound 3b was found to be active. (author)

  5. Designing new interfaces for ROOT data processing

    CERN Document Server

    Vuorinen, Kalle Elmer

    2016-01-01

    ROOT is a C++ framework for data analysis provided with a Python interface (PyRoot). ROOT is used in every Large Hadron Collider experiment. This project presents a way of reading ROOT TTree by using a new class called DataFrame, which allows the usage of cache and functional chains. Reading TTrees in Python has been quite slow compared to the C++ way of doing it and for this reason we also bring the possibility to read them with just-in-time (JIT) compiled C++ code, using another new Python class called TreeReader.

  6. Power Efficient Division and Square Root Unit

    DEFF Research Database (Denmark)

    Liu, Wei; Nannarelli, Alberto

    2012-01-01

    Although division and square root are not frequent operations, most processors implement them in hardware to not compromise the overall performance. Two classes of algorithms implement division or square root: digit-recurrence and multiplicative (e.g., Newton-Raphson) algorithms. Previous work...... shows that division and square root units based on the digit-recurrence algorithm offer the best tradeoff delay-area-power. Moreover, the two operations can be combined in a single unit. Here, we present a radix-16 combined division and square root unit obtained by overlapping two radix-4 stages...

  7. The significance of ecology in the development of Verticillium chlamydosporium as a biological control agent against root-knot nematodes (Meloidogyne spp.)

    OpenAIRE

    Leij, de, F.A.A.M.

    1992-01-01

    A thorough understanding of the interactions which occur between nematode parasites and nematode pests and the influence of biotic and abiotic factors on these interactions, is essential in the development of biological control agents for nematodes. The aim of this study was to develop a particular isolate of the nematophagous fungus <em>Verticillium chlamydosporiumem> as a biological control agent for root-knot nematodes. The work has gained insight into some of the ke...

  8. ROOT HYDRAULIC CONDUCTIVITY AND PHOTOSYNTHETIC CAPACITY OF EUCALYPT CLONAL CUTTINGS WITH ROOT MALFORMATION INDUCTIONS

    Directory of Open Access Journals (Sweden)

    Fábio Afonso Mazzei Moura de Assis Figueiredo

    2014-06-01

    Full Text Available http://dx.doi.org/10.5902/1980509814566The gain reduction of wood biomass in trees has been assigned to root deformations even in the nursery phase. The objective of this work was the evaluation of the root system hydraulic conductivity, gas exchanges and photochemical efficiency of eucalypt clonal cuttings with and without root deformation inductions. The treatments were: 1 operational cuttings without root malformation inductions (grown according to the used methodology of Fibria Cellulose S.A.; 2 root deformation inductions. These inductions did not promote decrease in the root volume. However, the deformations brought reduction of the root system hydraulic conductivity. Lower photosynthetic rates were also observed along the day in the cuttings in the root deformed cuttings. This decreasing rate is connected to stomatal and non stomatal factors.

  9. Root-growth-inhibiting sheet

    Science.gov (United States)

    Burton, Frederick G.; Cataldo, Dominic A.; Cline, John F.; Skiens, W. Eugene; Van Voris, Peter

    1993-01-01

    In accordance with this invention, a porous sheet material is provided at intervals with bodies of a polymer which contain a 2,6-dinitroaniline. The sheet material is made porous to permit free passage of water. It may be either a perforated sheet or a woven or non-woven textile material. A particularly desirable embodiment is a non-woven fabric of non-biodegradable material. This type of material is known as a "geotextile" and is used for weed control, prevention of erosion on slopes, and other landscaping purposes. In order to obtain a root repelling property, a dinitroaniline is blended with a polymer which is attached to the geotextile or other porous material.

  10. Piriformospora indica root colonization triggers local and systemic root responses and inhibits secondary colonization of distal roots.

    Directory of Open Access Journals (Sweden)

    Lorenzo Pedrotti

    Full Text Available Piriformosporaindica is a basidiomycete fungus colonizing roots of a wide range of higher plants, including crop plants and the model plant Arabidopsis thaliana. Previous studies have shown that P. indica improves growth, and enhances systemic pathogen resistance in leaves of host plants. To investigate systemic effects within the root system, we established a hydroponic split-root cultivation system for Arabidopsis. Using quantitative real-time PCR, we show that initial P. indica colonization triggers a local, transient response of several defense-related transcripts, of which some were also induced in shoots and in distal, non-colonized roots of the same plant. Systemic effects on distal roots included the inhibition of secondary P. indica colonization. Faster and stronger induction of defense-related transcripts during secondary inoculation revealed that a P. indica pretreatment triggers root-wide priming of defense responses, which could cause the observed reduction of secondary colonization levels. Secondary P. indica colonization also induced defense responses in distant, already colonized parts of the root. Endophytic fungi therefore trigger a spatially specific response in directly colonized and in systemic root tissues of host plants.

  11. Piriformospora indica root colonization triggers local and systemic root responses and inhibits secondary colonization of distal roots.

    Science.gov (United States)

    Pedrotti, Lorenzo; Mueller, Martin J; Waller, Frank

    2013-01-01

    Piriformosporaindica is a basidiomycete fungus colonizing roots of a wide range of higher plants, including crop plants and the model plant Arabidopsis thaliana. Previous studies have shown that P. indica improves growth, and enhances systemic pathogen resistance in leaves of host plants. To investigate systemic effects within the root system, we established a hydroponic split-root cultivation system for Arabidopsis. Using quantitative real-time PCR, we show that initial P. indica colonization triggers a local, transient response of several defense-related transcripts, of which some were also induced in shoots and in distal, non-colonized roots of the same plant. Systemic effects on distal roots included the inhibition of secondary P. indica colonization. Faster and stronger induction of defense-related transcripts during secondary inoculation revealed that a P. indica pretreatment triggers root-wide priming of defense responses, which could cause the observed reduction of secondary colonization levels. Secondary P. indica colonization also induced defense responses in distant, already colonized parts of the root. Endophytic fungi therefore trigger a spatially specific response in directly colonized and in systemic root tissues of host plants.

  12. Plant root research: the past, the present and the future

    OpenAIRE

    Lux, Alexander; Rost, Thomas L.

    2012-01-01

    This special issue is dedicated to root biologists past and present who have been exploring all aspects of root structure and function with an extensive publication record going over 100 years. The content of the Special Issue on Root Biology covers a wide scale of contributions, spanning interactions of roots with microorganisms in the rhizosphere, the anatomy of root cells and tissues, the subcellular components of root cells, and aspects of metal accumulation and stresses on root function ...

  13. Genetic ablation of root cap cells in Arabidopsis

    OpenAIRE

    Tsugeki, Ryuji; Fedoroff, Nina V.

    1999-01-01

    The root cap is increasingly appreciated as a complex and dynamic plant organ. Root caps sense and transmit environmental signals, synthesize and secrete small molecules and macromolecules, and in some species shed metabolically active cells. However, it is not known whether root caps are essential for normal shoot and root development. We report the identification of a root cap-specific promoter and describe its use to genetically ablate root caps by directing root cap-specific expression of...

  14. Large aortic root pseudoaneurysm occurring late after aortic root repair and valve replacement for endocarditis

    Directory of Open Access Journals (Sweden)

    Prashanth Panduranga

    2013-01-01

    Full Text Available A 68-year-old male presented with Group B Streptococcus aortic valve (AV endocarditis with aortic root abscess and refractory sepsis. An emergency cardiac surgery was performed with root abscess drainage, excision and debridement of necrotic tissue, reconstruction of annulus, and AV replacement. Fifteen months later he presented with a huge aortic root pseudoaneurysm (PA. This case illustrates late occurrence of aortic root PA following AV surgery for endocarditis.

  15. Fine root production at drained peatland sites

    Energy Technology Data Exchange (ETDEWEB)

    Finer, L. [Finnish Forest Research Inst. (Finland). Joensuu Research Station; Laine, J. [Helsinki Univ. (Finland). Dept. of Forest Ecology

    1996-12-31

    The preliminary results of the Finnish project `Carbon balance of peatlands and climate change` show that fine roots play an important role in carbon cycling on peat soils. After drainage the roots of mire species are gradually replaced by the roots of trees and other forest species. Pine fine root biomass reaches a maximum level by the time of crown closure, some 20 years after drainage on pine mire. The aim of this study is to compare the results of the sequential coring method and the ingrowth bag method used for estimating fine root production on three drained peatland sites of different fertility. The results are preliminary and continuation to the work done in the study Pine root production on drained peatlands, which is part of the Finnish project `Carbon cycling on peatlands and climate change`. In this study the fine root biomass was greater on the poor site than on the rich sites. Pine fine root production increased with the decrease in fertility. Root turnover and the production of field layer species were greater on the rich sites than on the poor site. The results suggested that the in growth bag method measured more root activity than the magnitude of production. More than two growing seasons would have been needed to balance the root dynamics in the in growth bags with the surrounding soil. That time would probably have been longer on the poor site than on the rich ones and longer for pine and field layer consisting of dwarf shrubs than for field layer consisting of sedge like species and birch. (11 refs.)

  16. Low Light Availability Alters Root Exudation and Reduces Putative Beneficial Microorganisms in Seagrass Roots

    Directory of Open Access Journals (Sweden)

    Belinda C. Martin

    2018-01-01

    Full Text Available Seagrass roots host a diverse microbiome that is critical for plant growth and health. Composition of microbial communities can be regulated in part by root exudates, but the specifics of these interactions in seagrass rhizospheres are still largely unknown. As light availability controls primary productivity, reduced light may impact root exudation and consequently the composition of the root microbiome. Hence, we analyzed the influence of light availability on root exudation and community structure of the root microbiome of three co-occurring seagrass species, Halophila ovalis, Halodule uninervis and Cymodocea serrulata. Plants were grown under four light treatments in mesocosms for 2 weeks; control (100% surface irradiance (SI, medium (40% SI, low (20% SI and fluctuating light (10 days 20% and 4 days 100%. 16S rDNA amplicon sequencing revealed that microbial diversity, composition and predicted function were strongly influenced by the presence of seagrass roots, such that root microbiomes were unique to each seagrass species. Reduced light availability altered seagrass root exudation, as characterized using fluorescence spectroscopy, and altered the composition of seagrass root microbiomes with a reduction in abundance of potentially beneficial microorganisms. Overall, this study highlights the potential for above-ground light reduction to invoke a cascade of changes from alterations in root exudation to a reduction in putative beneficial microorganisms and, ultimately, confirms the importance of the seagrass root environment – a critical, but often overlooked space.

  17. Effects of rooting media on root growth and morphology of Brassica ...

    African Journals Online (AJOL)

    Seedlings of the two B. rapa genotypes were supplied with nutrients on the nutrient conducting papers and in the soil-filled boxes. The papers and soil-filled boxes were fixed to flatbed scanners and two-dimensional images of roots were periodically taken and analysed. Root media effects on shoot and root biomass and on ...

  18. Root form and clinical radiographic estimation of the number of root ...

    African Journals Online (AJOL)

    The root form of 100 extracted maxillary premolars, the pre-operative radiographic estimation and clinical radiographic determination of the number of root canals in 340 maxillary premolars of Nigerian patients attending the dental hospital for endodontic treatment were studied. The maxillary second premolars had one root ...

  19. Kinetics of short-term root-carbon mineralization in roots of biofuel crops in soils

    Science.gov (United States)

    To better understand and document the rates of root decomposition in biofuel cropping systems, we compared the evolution of CO2 from roots incubated with samples of two Iowa Mollisols. Root samples were collected from experimental plots for four cropping systems: a multispecies reconstructed prairie...

  20. Non-canonical WOX11-mediated root branching contributes to plasticity in Arabidopsis root system architecture.

    Science.gov (United States)

    Sheng, Lihong; Hu, Xiaomei; Du, Yujuan; Zhang, Guifang; Huang, Hai; Scheres, Ben; Xu, Lin

    2017-09-01

    Lateral roots (LRs), which originate from the growing root, and adventitious roots (ARs), which are formed from non-root organs, are the main contributors to the post-embryonic root system in Arabidopsis However, our knowledge of how formation of the root system is altered in response to diverse inductive cues is limited. Here, we show that WOX11 contributes to root system plasticity. When seedlings are grown vertically on medium, WOX11 is not expressed in LR founder cells. During AR initiation, WOX11 is expressed in AR founder cells and activates LBD16 LBD16 also functions in LR formation and is activated in that context by ARF7 / 19 and not by WOX11 This indicates that divergent initial processes that lead to ARs and LRs may converge on a similar mechanism for primordium development. Furthermore, we demonstrated that when plants are grown in soil or upon wounding on medium, the primary root is able to produce both WOX11 -mediated and non- WOX11 -mediated roots. The discovery of WOX11 -mediated root-derived roots reveals a previously uncharacterized pathway that confers plasticity during the generation of root system architecture in response to different inductive cues. © 2017. Published by The Company of Biologists Ltd.

  1. Effect of Root Moisture Content and Diameter on Root Tensile Properties

    Science.gov (United States)

    Yang, Yuanjun; Chen, Lihua; Li, Ning; Zhang, Qiufen

    2016-01-01

    The stabilization of slopes by vegetation has been a topical issue for many years. Root mechanical characteristics significantly influence soil reinforcement; therefore it is necessary to research into the indicators of root tensile properties. In this study, we explored the influence of root moisture content on tensile resistance and strength with different root diameters and for different tree species. Betula platyphylla, Quercus mongolica, Pinus tabulaeformis, and Larix gmelinii, the most popular tree species used for slope stabilization in the rocky mountainous areas of northern China, were used in this study. A tensile test was conducted after root samples were grouped by diameter and moisture content. The results showedthat:1) root moisture content had a significant influence on tensile properties; 2) slightly loss of root moisture content could enhance tensile strength, but too much loss of water resulted in weaker capacity for root elongation, and consequently reduced tensile strength; 3) root diameter had a strong positive correlation with tensile resistance; and4) the roots of Betula platyphylla had the best tensile properties when both diameter and moisture content being controlled. These findings improve our understanding of root tensile properties with root size and moisture, and could be useful for slope stabilization using vegetation. PMID:27003872

  2. Root diversity in alpine plants: root length, tensile strength and plant age

    Science.gov (United States)

    Pohl, M.; Stroude, R.; Körner, C.; Buttler, A.; Rixen, C.

    2009-04-01

    A high diversity of plant species and functional groups is hypothesised to increase the diversity of root types and their subsequent effects for soil stability. However, even basic data on root characteristics of alpine plants are very scarce. Therefore, we determined important root characteristics of 13 plant species from different functional groups, i.e. grasses, herbs and shrubs. We excavated the whole root systems of 62 plants from a machine-graded ski slope at 2625 m a.s.l. and analysed the rooting depth, the horizontal root extension, root length and diameter. Single roots of plant species were tested for tensile strength. The age of herbs and shrubs was determined by growth-ring analysis. Root characteristics varied considerably between both plant species and functional groups. The rooting depth of different species ranged from 7.2 ± 0.97 cm to 20.5 ± 2.33 cm, but was significantly larger in the herb Geum reptans (70.8 ± 10.75 cm). The woody species Salix breviserrata reached the highest horizontal root extensions (96.8 ± 25.5 cm). Most plants had their longest roots in fine diameter classes (0.5

  3. Effect of Root Moisture Content and Diameter on Root Tensile Properties.

    Science.gov (United States)

    Yang, Yuanjun; Chen, Lihua; Li, Ning; Zhang, Qiufen

    2016-01-01

    The stabilization of slopes by vegetation has been a topical issue for many years. Root mechanical characteristics significantly influence soil reinforcement; therefore it is necessary to research into the indicators of root tensile properties. In this study, we explored the influence of root moisture content on tensile resistance and strength with different root diameters and for different tree species. Betula platyphylla, Quercus mongolica, Pinus tabulaeformis, and Larix gmelinii, the most popular tree species used for slope stabilization in the rocky mountainous areas of northern China, were used in this study. A tensile test was conducted after root samples were grouped by diameter and moisture content. The results showedthat:1) root moisture content had a significant influence on tensile properties; 2) slightly loss of root moisture content could enhance tensile strength, but too much loss of water resulted in weaker capacity for root elongation, and consequently reduced tensile strength; 3) root diameter had a strong positive correlation with tensile resistance; and4) the roots of Betula platyphylla had the best tensile properties when both diameter and moisture content being controlled. These findings improve our understanding of root tensile properties with root size and moisture, and could be useful for slope stabilization using vegetation.

  4. Air lateral root pruning affects longleaf pine seedling root system morphology

    Science.gov (United States)

    Shi-Jean Susana Sung; Dave Haywood

    2016-01-01

    Longleaf pine (Pinus palustris) seedlings were cultured with air lateral root pruning (side-vented containers, VT) or without (solid-walled containers, SW). Seedling root system morphology and growth were assessed before planting and 8 and 14 months after planting. Although VT seedlings had greater root collar diameter than the SW before planting,...

  5. X-ray computed tomography uncovers root-root interactions: quantifying spatial relationships between interacting root systems in three dimensions

    Directory of Open Access Journals (Sweden)

    Alexander Martin Paya

    2015-04-01

    Full Text Available Research in the field of plant biology has recently demonstrated that inter- and intra-specific interactions belowground can dramatically alter root growth. Our aim was to answer questions related to the effect of inter- vs. intra-specific interactions on the growth and utilization of undisturbed space by fine roots within three dimensions (3D using micro X-ray computed tomography. To achieve this, Populus tremuloides (quaking aspen and Picea mariana (black spruce seedlings were planted into containers as either solitary individuals, or inter-/intra-specific pairs, allowed to grow for two months, and 3D metrics developed in order to quantify their use of belowground space. In both aspen and spruce, inter-specific root interactions produced a shift in the vertical distribution of the root system volume, and deepened the average position of root tips when compared to intra-specifically growing seedlings. Inter-specific interactions also increased the minimum distance between root tips belonging to the same root system. There was no effect of belowground interactions on the radial distribution of roots, or the directionality of lateral root growth for either species. In conclusion, we found that significant differences were observed more often when comparing controls (solitary individuals and paired seedlings (inter- or intra-specific, than when comparing inter- and intra-specifically growing seedlings. This would indicate that competition between neighboring seedlings was more responsible for shifting fine root growth in both species than was neighbor identity. However, significant inter- vs. intra-specific differences were observed, which further emphasizes the importance of biological interactions in competition studies.

  6. DISTRIBUIÇÃO MENSAL E ATIVIDADE HORÁRIA DE Anopheles (DIPTERA: CULICIDAE EM UMA ÁREA RURAL DA AMAZÔNIA ORIENTAL.

    Directory of Open Access Journals (Sweden)

    Ricardo Marcelo dos Anjos Ferreira

    2013-12-01

    Full Text Available A investigação tem como objetivo caracterizar a distribuição mensal de espécies anofélicas e sua frequência horária na Comunidade São José do Mata Fome, área rural de Macapá-AP. As coletas foram realizadas entre fevereiro de 2008 a janeiro de 2009 com uso de duas armadilhas de Shannon, sendo a primeira instalada em ambiente de mata de galeria e a segunda no peridomicílio, nos horários de18:00h às 24:00h. Após a coleta, o material foi acondicionado em frascos plásticos e transportado até o laboratório de Arhropoda da Universidade Federal do Amapá e posteriormente submetido à identificação. Totalizaram 6435 exemplares registrados, sendo 4471 (69,48% noperidomicílio e 1964 (30,52% na mata. As espécies mais abundantes foram: An. braziliensis (35,68%, An. nuneztovari (22,89%, An. peryassui (13,63%, An. marajoara (12,84%, An. darlingi (7,74% e 7,24% outras espécies. Em relação à frequência horária, os anofelinos supracitados apresentaram variações, tanto no peridomicílio quanto na mata, em seuspicos de abundância. Os resultados obtidos contribuirão para o conhecimento da diversidade de Anopheles no Estado do Amapá, possibilitando o incremento de informações sobre a distribuição dessas espécies e sua capacidade vetorial com relação à transmissão de malária, visando com isso, eficácia nas medidas de controle. Palavras-chave: Abundância, Malária, Sazonalidade, Shannon. DOI: http://dx.doi.org/10.18561/2179-5746/biotaamazonia.v3n3p64-75

  7. Coupling root architecture and pore network modeling - an attempt towards better understanding root-soil interactions

    Science.gov (United States)

    Leitner, Daniel; Bodner, Gernot; Raoof, Amir

    2013-04-01

    Understanding root-soil interactions is of high importance for environmental and agricultural management. Root uptake is an essential component in water and solute transport modeling. The amount of groundwater recharge and solute leaching significantly depends on the demand based plant extraction via its root system. Plant uptake however not only responds to the potential demand, but in most situations is limited by supply form the soil. The ability of the plant to access water and solutes in the soil is governed mainly by root distribution. Particularly under conditions of heterogeneous distribution of water and solutes in the soil, it is essential to capture the interaction between soil and roots. Root architecture models allow studying plant uptake from soil by describing growth and branching of root axes in the soil. Currently root architecture models are able to respond dynamically to water and nutrient distribution in the soil by directed growth (tropism), modified branching and enhanced exudation. The porous soil medium as rooting environment in these models is generally described by classical macroscopic water retention and sorption models, average over the pore scale. In our opinion this simplified description of the root growth medium implies several shortcomings for better understanding root-soil interactions: (i) It is well known that roots grow preferentially in preexisting pores, particularly in more rigid/dry soil. Thus the pore network contributes to the architectural form of the root system; (ii) roots themselves can influence the pore network by creating preferential flow paths (biopores) which are an essential element of structural porosity with strong impact on transport processes; (iii) plant uptake depend on both the spatial location of water/solutes in the pore network as well as the spatial distribution of roots. We therefore consider that for advancing our understanding in root-soil interactions, we need not only to extend our root models

  8. Enraizamento de estacas caulinares e radiculares de Rubus fruticosus tratadas com AIB Rooting of stems and root cuttings of Rubus fruticosus using IBA

    Directory of Open Access Journals (Sweden)

    Maraísa Hellen Tadeu

    2012-12-01

    Full Text Available O objetivo deste trabalho foi avaliar o enraizamento de estacas caulinares e radiculares de Rubus fruticosus, tratadas com AIB. Estacas radiculares e caulinares dessa espécie foram coletadas em março, tendo posteriormente suas bases tratadas com diferentes concentrações de ácido indolbutírico (AIB, 1000, 2000, 3000 e 4000 mg L-1 por 10 segundos. O tratamento controle foi baseado somente no uso da água. As estacas caulinares foram enterradas a 2/3 de seu comprimento, em posição vertical, e as radiculares foram totalmente enterradas, na horizontal, utilizando-se a vermiculitaï como substrato, em telado com sombreamento de 50%. Após 60 dias, pôde-se concluir que as estacas caulinares apresentaram melhores resultados para formação das mudas, não se devendo tratá-las com AIB.The objective of the present work was to evaluate the rizogenic potential for stems and root cuttings of Rubus fruticosus treated with indolbutyric acid. Roots and stems cuttings of this specie were collected in March, and had their basal portions treated with different concentrations of indolbutyric acid (IBA (1000, 2000, 3000 and 4000 mg L-1 for 10 seconds. The control treatment used only water. The stem cuttings were planted with 2/3 of their length in the vertical position and the root cuttings were totally covered in the horizontal position, using vermiculite as substrate, in screenhouse with 50% of shade. After 60 days, the stem cuttings presented better results for seedling formation, without IBA.

  9. Root-flipped multiband refocusing pulses.

    Science.gov (United States)

    Sharma, Anuj; Lustig, Michael; Grissom, William A

    2016-01-01

    To design low peak power multiband refocusing radiofrequency pulses, with application to simultaneous multislice spin echo MRI. Multiband Shinnar-Le Roux β polynomials were designed using convex optimization. A Monte Carlo algorithm was used to determine patterns of β polynomial root flips that minimized the peak power of the resulting refocusing pulses. Phase-matched multiband excitation pulses were also designed to obtain linear-phase spin echoes. Simulations compared the performance of the root-flipped pulses with time-shifted and phase-optimized pulses. Phantom and in vivo experiments at 7T validated the function of the root-flipped pulses and compared them to time-shifted spin echo signal profiles. Averaged across number of slices, time-bandwidth product, and slice separation, the root-flipped pulses have 46% shorter durations than time-shifted pulses with the same peak radiofrequency amplitude. Unlike time-shifted and phase-optimized pulses, the root-flipped pulses' excitation errors do not increase with decreasing band separation. Experiments showed that the root-flipped pulses excited the desired slices at the target locations, and that for equivalent slice characteristics, the shorter root-flipped pulses allowed shorter echo times, resulting in higher signal than time-shifted pulses. The proposed root-flipped multiband radiofrequency pulse design method produces low peak power pulses for simultaneous multislice spin echo MRI. © 2015 Wiley Periodicals, Inc.

  10. Interactions of Root Disease and Bark Beetles

    Science.gov (United States)

    George T. Ferrell; J. Richard Parmeter Jr.

    1989-01-01

    Associations between root diseases and bark beetles (Scolytidae) constitute some of the most serious pest complexes affecting forests in North America and elsewhere. The interactive functioning of these pests derives from the following relationships: 1) root diseases predispose trees to bark beetle infestation by lowering resistance, and perhaps...

  11. Valve-sparing aortic root replacement†

    NARCIS (Netherlands)

    Koolbergen, David R.; Manshanden, Johan S. J.; Bouma, Berto J.; Blom, Nico A.; Mulder, Barbara J. M.; de Mol, Bas A. J. M.; Hazekamp, Mark G.

    2015-01-01

    To evaluate our results of valve-sparing aortic root replacement and associated (multiple) valve repair. From September 2003 to September 2013, 97 patients had valve-sparing aortic root replacement procedures. Patient records and preoperative, postoperative and recent echocardiograms were reviewed.

  12. Rapid phenotyping of alfalfa root system architecture

    Science.gov (United States)

    Root system architecture (RSA) influences the capacity of an alfalfa plant for symbiotic nitrogen fixation, nutrient uptake and water use efficiency, resistance to frost heaving, winterhardiness, and some pest and pathogen resistance. However, we currently lack a basic understanding of root system d...

  13. On the Denesting of Nested Square Roots

    Science.gov (United States)

    Gkioulekas, Eleftherios

    2017-01-01

    We present the basic theory of denesting nested square roots, from an elementary point of view, suitable for lower level coursework. Necessary and sufficient conditions are given for direct denesting, where the nested expression is rewritten as a sum of square roots of rational numbers, and for indirect denesting, where the nested expression is…

  14. Enhancing rooting consistency in Rosa damascena scions

    African Journals Online (AJOL)

    use

    2011-11-21

    Nov 21, 2011 ... significance. This experiment results were analyzed and illustrated in two ways; first, data were exposed to observation- the estimation of different IBA concentrations and mineral salt strengths on percentage of root formation and mean number of rooting either alone; and secondly, the effects of selected IBA ...

  15. Graphing Powers and Roots of Complex Numbers.

    Science.gov (United States)

    Embse, Charles Vonder

    1993-01-01

    Using De Moivre's theorem and a parametric graphing utility, examines powers and roots of complex numbers and allows students to establish connections between the visual and numerical representations of complex numbers. Provides a program to numerically verify the roots of complex numbers. (MDH)

  16. Root pattern: Shooting in the dark?

    NARCIS (Netherlands)

    Scheres, B.J.G.; Dolan, L.

    1998-01-01

    Root pattern formation takes place in the embryo and is propagated through subsequent growth and development of the seedling root meristem. Pattern is maintained by positional cues and in some cases by local cell interactions. Such interactions are involved in the balance between cell division and

  17. Roots, plant production and nutrient use efficiency

    NARCIS (Netherlands)

    Willigen, de P.; Noordwijk, van M.

    1987-01-01

    The role of roots in obtaining high crop production levels as well as a high nutrient use efficiency is discussed. Mathematical models of diffusion and massflow of solutes towards roots are developed for a constant daily uptake requirement. Analytical solutions are given for simple and more

  18. An intersection test for panel unit roots

    NARCIS (Netherlands)

    Hanck, C.

    2013-01-01

    This article proposes a new panel unit root test based on Simes' ( 1986) classical intersection test. The test is robust to general patterns of cross-sectional dependence and yet is straightforward to implement, only requiring p-values of time series unit root tests of the series in the panel, and

  19. Method for Constructing Standardized Simulated Root Canals.

    Science.gov (United States)

    Schulz-Bongert, Udo; Weine, Franklin S.

    1990-01-01

    The construction of visual and manipulative aids, clear resin blocks with root-canal-like spaces, for simulation of root canals is explained. Time, materials, and techniques are discussed. The method allows for comparison of canals, creation of any configuration of canals, and easy presentation during instruction. (MSE)

  20. On König's root finding algorithms

    DEFF Research Database (Denmark)

    Buff, Xavier; Henriksen, Christian

    2003-01-01

    In this paper, we first recall the definition of a family of root-finding algorithms known as König's algorithms. We establish some local and some global properties of those algorithms. We give a characterization of rational maps which arise as König's methods of polynomials with simple roots. We...

  1. 33 CFR 117.1095 - Root River.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Root River. 117.1095 Section 117.1095 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Wisconsin § 117.1095 Root River. (a) The draw of the Main Street...

  2. Forest root diseases across the United States

    Science.gov (United States)

    I. Blakey Lockman; Holly S. J. Kearns

    2016-01-01

    The increasing importance and impacts of root diseases on the forested ecosystems across the United States are documented in this report. Root diseases have long-term impacts on the ecosystems where they reside due to their persistence onsite. As a group of agents, they are a primary contributor to overall risk of growth loss and mortality of trees in the lower 48...

  3. Emergency Medical Service (EMS) Stations

    Data.gov (United States)

    Kansas Data Access and Support Center — EMS Locations in Kansas The EMS stations dataset consists of any location where emergency medical services (EMS) personnel are stationed or based out of, or where...

  4. Bilateral mandibular second premolars with three separate roots

    Directory of Open Access Journals (Sweden)

    Raed Hakam Mukhaimer

    2012-01-01

    Full Text Available The mandibular second premolar is usually described as a single-rooted tooth with a single root canal. However, three root canals may be found but the occurrence of three separate roots is extremely rare. This report describes the case of a patient with bilateral mandibular second premolar with three roots and three root canals. The pre-operative radiograph of the right premolar revealed the presence of three roots, while the radiograph of the left premolar looked unusual and showed two roots. The access cavity preparation was extended and three orifices leading to three roots were found. All root canals were cleaned, shaped, and obturated. The post-operative radiograph showed a satisfactory root canal filling. Clinicians should always consider the presence of anatomical variations in the teeth during endodontic treatments. Despite the low prevalence, variations may occur in the number of roots and root canals of mandibular second premolars, as demonstrated in this case report.

  5. GiA Roots: software for the high throughput analysis of plant root system architecture

    Science.gov (United States)

    2012-01-01

    Background Characterizing root system architecture (RSA) is essential to understanding the development and function of vascular plants. Identifying RSA-associated genes also represents an underexplored opportunity for crop improvement. Software tools are needed to accelerate the pace at which quantitative traits of RSA are estimated from images of root networks. Results We have developed GiA Roots (General Image Analysis of Roots), a semi-automated software tool designed specifically for the high-throughput analysis of root system images. GiA Roots includes user-assisted algorithms to distinguish root from background and a fully automated pipeline that extracts dozens of root system phenotypes. Quantitative information on each phenotype, along with intermediate steps for full reproducibility, is returned to the end-user for downstream analysis. GiA Roots has a GUI front end and a command-line interface for interweaving the software into large-scale workflows. GiA Roots can also be extended to estimate novel phenotypes specified by the end-user. Conclusions We demonstrate the use of GiA Roots on a set of 2393 images of rice roots representing 12 genotypes from the species Oryza sativa. We validate trait measurements against prior analyses of this image set that demonstrated that RSA traits are likely heritable and associated with genotypic differences. Moreover, we demonstrate that GiA Roots is extensible and an end-user can add functionality so that GiA Roots can estimate novel RSA traits. In summary, we show that the software can function as an efficient tool as part of a workflow to move from large numbers of root images to downstream analysis. PMID:22834569

  6. Effect of lead on root growth

    Science.gov (United States)

    Fahr, Mouna; Laplaze, Laurent; Bendaou, Najib; Hocher, Valerie; Mzibri, Mohamed El; Bogusz, Didier; Smouni, Abdelaziz

    2013-01-01

    Lead (Pb) is one of the most widespread heavy metal contaminant in soils. It is highly toxic to living organisms. Pb has no biological function but can cause morphological, physiological, and biochemical dysfunctions in plants. Plants have developed a wide range of tolerance mechanisms that are activated in response to Pb exposure. Pb affects plants primarily through their root systems. Plant roots rapidly respond either (i) by the synthesis and deposition of callose, creating a barrier that stops Pb entering (ii) through the uptake of large amounts of Pb and its sequestration in the vacuole accompanied by changes in root growth and b