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Sample records for cyprinids actinopterygii cyprinidae

  1. Phylogenetic relationships of the algae scraping cyprinid genus Capoeta (Teleostei: Cyprinidae)

    Czech Academy of Sciences Publication Activity Database

    Levin, B. A.; Freyhof, J.; Lajbner, Zdeněk; Perea, S.; Abdoli, A.; Gaffaroglu, M.; Özulog, M.; Rubeyan, H.R.; Salnikov, V.B.; Doadrio, I.

    2012-01-01

    Roč. 62, č. 1 (2012), s. 542-549 ISSN 1055-7903 R&D Projects: GA MŠk LC06073 Institutional research plan: CEZ:AV0Z50450515 Keywords : Cyprinidae * Phylogeny * Polyploid barbini Subject RIV: EH - Ecology, Behaviour Impact factor: 4.066, year: 2012 http://www.sciencedirect.com/science/article/pii/S1055790311003940

  2. REARING OF PELED (COREGONUS PELED Gmelin IN POLYCULTURE WITH CYPRINIDS (CYPRINIDAE AND STURGEONS (ACIPENSERIDAE

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    G. Kurinenko

    2016-03-01

    Full Text Available Purpose. To analyze the results of rearing and provide aquaculture-biological characteristic of peled reared in polyculture with sturgeons and cyprinids based on pond technology. Methodology. The material for the studies were fry, young-of-the-year, yearlings and age-1+ peled produced from eggs exported in March 2009 from Russian Federation. Rearing of peled was carried out based on the technology developed by the All-Union Scientific and Research Institute of Pond Fish Culture for coregonids with the use of methodical recommendations on the biotechnology of industrial rearing of seed coregonids. Studies were carried out at the pond fish farm “Korop” of Lviv region. Water supply of rearing ponds was done by self-flow. The investigation of fish diet and hydrobiological studies were carried out using conventional methods. Findings. We performed a study of fish egg incubation and produced larvae with their further rearing in floating cages to the fingerling stage. Rearing of peled in polyculture allows increasing the fish productivity parameters at the first year of rearing by 1.3%, at the second year by 0.9%. Average weights of age-1 and age-1+ peled were 185.3 g and 450 g, respectively. In these rearing conditions, daily growth of the young-of-the-year was within 0.1-1.5 g, age-1+ – 1.1-3.3 g. As a positive result of rearing, we should note high weight gain during winter period that was more than 50%. We also investigated qualitative and quantitative composition of zooplankton and peled juvenile diet. Originality. The works of peled rearing based on pond technology in polyculture with sturgeons and cyprinids were carried out in the conditions of Ukraine for the first time. Practical value. The results of the performed works along with similar previous works on peled rearing in ponds will be used for the creation of methodical recommendations on rearing of peled seeds, which will be used by Ukrainian fish farms in future.

  3. A new species of Algansea (Actinopterygii: Cyprinidae from the Ameca River basin, in Central Mexico Una especie nueva de Algansea (Actinopterygii: Cyprinidae en la cuenca del río Ameca en el centro de México

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    Rodolfo Pérez-Rodríguez

    2009-08-01

    Full Text Available A morphological comparative analysis was performed among different populations of the cyprinid Algansea tincella Valenciennes, 1844 from the Lerma-Chapala and Ameca River basins in central Mexico. A new species, Algansea amecae n. sp. is described from individuals collected from small tributary in the headwaters of the Ameca basin. The new species differs from Lerma-Chapala populations of A. tincella by having a lower number of transversal scales, a lower number of infraorbital pores, a prominent dark lateral stripe along the body, a black caudal spot extending onto the medial caudal inter-radial membranes, and a pigmented ("dotted" lateral line. This new species increases the high level of endemism in the freshwater ichthyofauna of the Ameca basin. It appears to be most closely related to populations in the Lerma-Chapala-Santiago system, as is the case for several other species in the Ameca basin. This pattern of relationship provides evidence for a historical connection between the 2 basins, and implies that a vicariance event led to the isolation of populations and a subsequent speciation event. Due to the limited distributional range of Algansea amecae n. sp., and the environmental deterioration of the Ameca River, we propose that this new species should be designated as a protected species under Mexican law.Se realizó un análisis morfológico comparando diferentes poblaciones del ciprínido Algansea tincella Valenciennes, 1844 correspondientes a los sistemas hidrológicos Lerma-Chapala y cuenca del río Ameca. Con base en este análisis se describe una nueva especie, Algansea amecae n. sp. a partir de los individuos recolectados en un pequeño afluente del alto Ameca, en el centro de México. La nueva especie difiere de las poblaciones de A. tincella del sistema Lerma-Chapala-Santiago por presentar un menor número de escamas transversales, un menor número de poros infraorbitales, una franja obscura lateral muy marcada a lo largo del

  4. First records of scale deformities in seven freshwater fish species (Actinopterygii: Percidae and Cyprinidae) collected from three ponds in the Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Rutkayová, J.; Jawad, L.; Nebesářová, Jana; Beneš, K.; Petrášková, E.; Näslund, J.

    2016-01-01

    Roč. 46, č. 3 (2016), s. 225-238 ISSN 0137-1592 Institutional support: RVO:60077344 Keywords : fish * scale abnormality * adverse environment * Cyprinidae * Percidae Subject RIV: EG - Zoology Impact factor: 0.670, year: 2016

  5. Histological-morphometric structure of the liver of some cyprinids (Cyprinidae Rafinesque, 1810 from the Zaporizhzhia reservoir

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    T. Sharamok

    2017-12-01

    Full Text Available Purpose. To investigate the state of the liver of wild carp (Cyprinus carpio Linnaeus, 1758 and bream (Abramis brama Linnaeus, 1758 from sites of the Zaporizhzhia reservoir with different human pressures based on histological and cytometric parameters Methodology.The study was carried out in two zones of the Zaporizhzhia reservoir with different degrees and types of human impact - the lower part, located in the agrarian zone (conditionally "environmentally clean" site, and in the Samara Bay with high content of heavy metals in water. The objects of the study were age-4 bream and wild carp. The fish livers for histological studies were obtained from freshly caught fish (in the spring-summer period by anatomical dissection. Fragments of organs of 0.3 – 0.5 cm were taken for preservation. For the preparation of histological preparations, the liver tissue was stained with hematoxylin and eosin. The pictures of histological preparations were made with the aid of a digital camera connected to the microscope. Histologic sections were investigated with 40X magnification using a microscope with the Sciencelab T500 5.17 M digital camera. Findings. Human impact on the hydroelectric system causes signs of hepatocyte hypertrophy and a number of histopathologies in cyprinids in the Samara Bay. The cytometric analysis of the liver showed that the area of hepatocytes in the bay was larger in comparison with the the individuals of similar age from the lower part of the reservoir by 20 and 10% for the wild carp and bream, respectively. In this case, a 14% increase in the small diameter of cells was observed the in hepatocytes of carp, and a 10% increase in large diameter and a 19% increase in nucleus area was observed in bream. The results of the histological invesatigation of the morphological structure of the liver of cyprinids inhabiting the Samara Bay have revealed a number of pathological changes. Fatty liver dystrophy was observed in 37% of bream and 12

  6. Molecular phylogenetics of the family Cyprinidae (Actinopterygii: Cypriniformes) as evidenced by sequence variation in the first intron of S7 ribosomal protein-coding gene: further evidence from a nuclear gene of the systematic chaos in the family.

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    He, Shunping; Mayden, Richard L; Wang, Xuzheng; Wang, Wei; Tang, Kevin L; Chen, Wei-Jen; Chen, Yiyu

    2008-03-01

    The family Cyprinidae is the largest freshwater fish group in the world, including over 200 genera and 2100 species. The phylogenetic relationships of major clades within this family are simply poorly understood, largely because of the overwhelming diversity of the group; however, several investigators have advanced different hypotheses of relationships that pre- and post-date the use of shared-derived characters as advocated through phylogenetic systematics. As expected, most previous investigations used morphological characters. Recently, mitochondrial DNA (mtDNA) sequences and combined morphological and mtDNA investigations have been used to explore and advance our understanding of species relationships and test monophyletic groupings. Limitations of these studies include limited taxon sampling and a strict reliance upon maternally inherited mtDNA variation. The present study is the first endeavor to recover the phylogenetic relationships of the 12 previously recognized monophyletic subfamilies within the Cyprinidae using newly sequenced nuclear DNA (nDNA) for over 50 species representing members of the different previously hypothesized subfamily and family groupings within the Cyprinidae and from other cypriniform families as outgroup taxa. Hypothesized phylogenetic relationships are constructed using maximum parsimony and Basyesian analyses of 1042 sites, of which 971 sites were variable and 790 were phylogenetically informative. Using other appropriate cypriniform taxa of the families Catostomidae (Myxocyprinus asiaticus), Gyrinocheilidae (Gyrinocheilus aymonieri), and Balitoridae (Nemacheilus sp. and Beaufortia kweichowensis) as outgroups, the Cyprinidae is resolved as a monophyletic group. Within the family the genera Raiamas, Barilius, Danio, and Rasbora, representing many of the tropical cyprinids, represent basal members of the family. All other species can be classified into variably supported and resolved monophyletic lineages, depending upon analysis

  7. Cenozoic tectonic and climatic events in southern Iberian Peninsula: Implications for the evolutionary history of freshwater fish of the genus Squalius (Actinopterygii, Cyprinidae).

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    Perea, Silvia; Cobo-Simon, Marta; Doadrio, Ignacio

    2016-04-01

    Southern Iberian freshwater ecosystems located at the border between the European and African plates represent a tectonically complex region spanning several geological ages, from the uplifting of the Betic Mountains in the Serravalian-Tortonian periods to the present. This area has also been subjected to the influence of changing climate conditions since the Middle-Upper Pliocene when seasonal weather patterns were established. Consequently, the ichthyofauna of southern Iberia is an interesting model system for analyzing the influence of Cenozoic tectonic and climatic events on its evolutionary history. The cyprinids Squalius malacitanus and Squalius pyrenaicus are allopatrically distributed in southern Iberia and their evolutionary history may have been defined by Cenozoic tectonic and climatic events. We analyzed MT-CYB (510 specimens) and RAG1 (140 specimens) genes of both species to reconstruct phylogenetic relationships and to estimate divergence times and ancestral distribution ranges of the species and their populations. We also assessed their levels of genetic structure and diversity as well as the amount of gene flow between populations. To investigate recent paleogeographical and climatic factors in southern Iberia, we modeled changes-through-time in sea level from the LGM to the present. Phylogenetic, geographic and population structure analyses revealed two well-supported species (S. malacitanus and S. pyrenaicus) in southern Iberia and two subclades (Atlantic and Mediterranean) within S. malacitanus. The origin of S. malacitanus and the separation of its Atlantic and Mediterranean populations occurred during the Serravalian-Tortonian and Miocene-Pliocene periods, respectively. These divergence events occurred in the Middle Pliocene and Pleistocene in S. pyrenaicus. In both species, Atlantic basins possessed populations with higher genetic diversity than Mediterranean, which may be explained by the Janda Lagoon. The isolation of S. malacitanus was

  8. Molecular evidence on the evolutionary and biogeographical patterns of European cyprinids.

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    Zardoya, R; Doadrio, I

    1999-08-01

    The phylogenetic relationships of 106 European cyprinid taxa were determined based on the complete nucleotide sequence (1140 bp) of the mitochondrial cytochrome b gene. The molecular phylogeny was used (1) to revise the current systematics of European cyprinids, (2) to establish the phylogenetic utility of traditional morphological characters that are widely used in Cyprinidae systematics, and (3) to discuss alternative hypotheses on the biogeography of the family in Europe. The age of the major lineages within European cyprinids was tentatively estimated with a molecular clock and showed full agreement with the fossil record of the group. Moreover, the results provided unambiguous evidence for a close phylogenetic affinity of some Caucasian and Greek endemic cyprinid taxa (e.g., B. capito and B. brachycephalus and Leuciscus keadicus, Barbus graecus, and B. albanicus, respectively) to Iberian and North African, but not Central European, cyprinids. The existence of such unexpected phylogenetic relationships refutes the classical hypothesis on the biogeography of European cyprinids, which assumes a dispersal of the cyprinid fauna from central Europe to southern Europe and northern Africa during the Miocene (and, hence, predicts a close phylogenetic relationship of all Caucasian, Greek, Iberian, and North African cyprinids to central European taxa). Instead, the existence of a Mediterranean realm independent of the central European route seems plausible based on the molecular evidence. It is likely that the new biogeographical scenario proposed here might apply to other primary freshwater European animals with low dispersal abilities, including fish, amphibians, and invertebrates.

  9. Genomic and transcriptomic approaches to study immunology in cyprinids: What is next?

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    Petit, Jules; David, Lior; Dirks, Ron; Wiegertjes, Geert F

    2017-10-01

    Accelerated by the introduction of Next-Generation Sequencing (NGS), a number of genomes of cyprinid fish species have been drafted, leading to a highly valuable collective resource of comparative genome information on cyprinids (Cyprinidae). In addition, NGS-based transcriptome analyses of different developmental stages, organs, or cell types, increasingly contribute to the understanding of complex physiological processes, including immune responses. Cyprinids are a highly interesting family because they comprise one of the most-diversified families of teleosts and because of their variation in ploidy level, with diploid, triploid, tetraploid, hexaploid and sometimes even octoploid species. The wealth of data obtained from NGS technologies provides both challenges and opportunities for immunological research, which will be discussed here. Correct interpretation of ploidy effects on immune responses requires knowledge of the degree of functional divergence between duplicated genes, which can differ even between closely-related cyprinid fish species. We summarize NGS-based progress in analysing immune responses and discuss the importance of respecting the presence of (multiple) duplicated gene sequences when performing transcriptome analyses for detailed understanding of complex physiological processes. Progressively, advances in NGS technology are providing workable methods to further elucidate the implications of gene duplication events and functional divergence of duplicates genes and proteins involved in immune responses in cyprinids. We conclude with discussing how future applications of NGS technologies and analysis methods could enhance immunological research and understanding. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  10. Methyltestosterone-induced changes in electro-olfactogram responses and courtship behaviors of cyprinids.

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    Belanger, Rachelle M; Pachkowski, Melanie D; Stacey, Norm E

    2010-01-01

    In the tinfoil barb (Barbonymus schwanenfeldii; family Cyprinidae), we previously found that increased olfactory sensitivity to a female prostaglandin pheromone could induce sexual behavior display in juvenile fish treated with androgens. Here, we determined if this phenomenon is widespread among cyprinid fishes by adding 17alpha-methyltestosterone (MT) to aquaria containing juveniles of 4 cyprinid species (tinfoil barbs; redtail sharkminnows, Epalzeorhynchos bicolor; goldfish, Carassius auratus; zebrafish, Danio rerio) and then using electro-olfactogram (EOG) recordings and behavioral assays to determine if androgen treatment enhances pheromone detection and male sex behaviors. In all 4 cyprinids, MT treatment increased the magnitudes and sensitivities of EOG response to prostaglandins and, consistent with our initial study on tinfoil barbs, did not affect EOG responses to the free and conjugated steroid to which each species is most sensitive. In zebrafish, EOG responses to prostaglandins were similar in MT-treated juveniles and adult males, whereas responses of control (ethanol exposed) fish were similar to those of adult females. Finally, as previously observed in tinfoil barbs, MT treatment of juvenile redtail sharkminnows increased courtship behaviors (nuzzling and quivering) with a stimulus fish. We conclude that androgen-induced increase in olfactory responsiveness to pheromonal prostaglandins is common among the family Cyprinidae. This phenomenon will help us unravel the development of sexually dimorphic olfactory-mediated behavior.

  11. Multiple source genes of HAmo SINE actively expanded and ongoing retroposition in cyprinid genomes relying on its partner LINE

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    Gan Xiaoni

    2010-04-01

    Full Text Available Abstract Background We recently characterized HAmo SINE and its partner LINE in silver carp and bighead carp based on hybridization capture of repetitive elements from digested genomic DNA in solution using a bead-probe 1. To reveal the distribution and evolutionary history of SINEs and LINEs in cyprinid genomes, we performed a multi-species search for HAmo SINE and its partner LINE using the bead-probe capture and internal-primer-SINE polymerase chain reaction (PCR techniques. Results Sixty-seven full-size and 125 internal-SINE sequences (as well as 34 full-size and 9 internal sequences previously reported in bighead carp and silver carp from 17 species of the family Cyprinidae were aligned as well as 14 new isolated HAmoL2 sequences. Four subfamilies (type I, II, III and IV, which were divided based on diagnostic nucleotides in the tRNA-unrelated region, expanded preferentially within a certain lineage or within the whole family of Cyprinidae as multiple active source genes. The copy numbers of HAmo SINEs were estimated to vary from 104 to 106 in cyprinid genomes by quantitative RT-PCR. Over one hundred type IV members were identified and characterized in the primitive cyprinid Danio rerio genome but only tens of sequences were found to be similar with type I, II and III since the type IV was the oldest subfamily and its members dispersed in almost all investigated cyprinid fishes. For determining the taxonomic distribution of HAmo SINE, inter-primer SINE PCR was conducted in other non-cyprinid fishes, the results shows that HAmo SINE- related sequences may disperse in other families of order Cypriniforms but absent in other orders of bony fishes: Siluriformes, Polypteriformes, Lepidosteiformes, Acipenseriformes and Osteoglossiforms. Conclusions Depending on HAmo LINE2, multiple source genes (subfamilies of HAmo SINE actively expanded and underwent retroposition in a certain lineage or within the whole family of Cyprinidae. From this

  12. Cyprinid fishes: systematics, biology, and exploitation

    National Research Council Canada - National Science Library

    Winfield, Ian J; Nelson, Joseph S

    1991-01-01

    The Cyprinidae is one of the two largest families of vertebrates in the world. This book draws together the experience of more than 30 international experts to provide the first comprehensive account of the biology of this family...

  13. Phylogenomic analysis resolves the formerly intractable adaptive diversification of the endemic clade of east Asian Cyprinidae (Cypriniformes.

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    Wenjing Tao

    Full Text Available Despite their great diversity and biological importance, evolutionary relationships among the endemic clade of East Asian Cyprinidae remain ambiguous. Understanding the phylogenetic history of this group involves many challenges. For instance, ecomorphological convergence may confound morphology-based phylogenetic inferences, and previous molecular phylogenetic studies based on single genes have often yielded contradictory and poorly supported trees. We assembled a comprehensive data matrix of 100 nuclear gene segments (∼ 71132 base pairs for representative species of the endemic East Asian cyprinid fauna and recovered a robust phylogeny from this genome-wide signal supported by multiple analytical methods, including maximum parsimony, maximum likelihood and Bayesian inference. Relaxed molecular clock analyses indicated species radiations of this clade concentrated at approximately 1.9-7.6 MYA. We provide evidence that the bursts of diversification in this fauna are directly linked to major paleoenvironmental events associated with monsoon evolution occurring from late Miocene to Pliocene. Ancestral state reconstruction reveals convergent morphological characters are hypothesized to be independent products of similar selective pressures in ecosystems. Our study is the first comprehensive phylogenetic study of the enigmatic East-Asian cyprinids. The explicit molecular phylogeny provides a valuable framework for future research in genome evolution, adaptation and speciation of cyprinids.

  14. Parasites as biological tags of divergence in Central European gudgeon populations (Actinopterygii: Cyprinidae: Gobioninae)

    Czech Academy of Sciences Publication Activity Database

    Kvach, Yuriy; Ondračková, Markéta; Bryjová, Anna; Jurajda, Pavel

    2017-01-01

    Roč. 72, č. 6 (2017), s. 671-679 ISSN 0006-3088 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:68081766 Keywords : Gobio * Romanogobio * sympatric species * parasite community * biological tags Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 0.759, year: 2016

  15. The food of roach, Rutilus rutilus (Actinopterygii: Cypriniformes: Cyprinidae), in a biomanipulated water supply reservoir

    Czech Academy of Sciences Publication Activity Database

    Zapletal, T.; Mareš, J.; Jurajda, Pavel; Všetičková, Lucie

    2014-01-01

    Roč. 44, č. 1 (2014), s. 15-22 ISSN 0137-1592 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:68081766 Keywords : diet analysis * gut * biomanipulation * age groups Subject RIV: EG - Zoology Impact factor: 0.577, year: 2014 http://www.aiep.pl/volumes/2010/5_1/pdf/03_1421_F1.pdf

  16. From Late Miocene to Holocene: processes of differentiation within the Telestes genus (Actinopterygii: Cyprinidae.

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    Vincent Dubut

    Full Text Available Investigating processes and timing of differentiation of organisms is critical in the understanding of the evolutionary mechanisms involved in microevolution, speciation, and macroevolution that generated the extant biodiversity. From this perspective, the Telestes genus is of special interest: the Telestes species have a wide distribution range across Europe (from the Danubian district to Mediterranean districts and have not been prone to translocation. Molecular data (mtDNA: 1,232 bp including the entire Cyt b gene; nuclear genome: 11 microsatellites were gathered from 34 populations of the Telestes genus, almost encompassing the entire geographic range. Using several phylogenetic and molecular dating methods interpreted in conjunction with paleoclimatic and geomorphologic evidence, we investigated the processes and timing of differentiation of the Telestes lineages. The observed genetic structure and diversity were largely congruent between mtDNA and microsatellites. The Messinian Salinity Crisis (Late Miocene seems to have played a major role in the speciation processes of the genus. Focusing on T. souffia, a species occurring in the Danube and Rhone drainages, we were able to point out several specific events from the Pleistocene to the Holocene that have likely driven the differentiation and the historical demography of this taxon. This study provides support for an evolutionary history of dispersal and vicariance with unprecedented resolution for any freshwater fish in this region.

  17. Early ontogeny of Labeo capensis (pisces: Cyprinidae)

    African Journals Online (AJOL)

    the early development of Indian Labeo species (e.g. Khan. 1925; Ahmad 1944 ...... ber, size or intensity, was helpful in grouping North American cyprinid larvae. .... impregnated eggs of common freshwater fishes of Bengal. Curro Sci. 26: 125 ...

  18. Devario in Bangladesh: Species diversity, sibling species, and introgression within danionin cyprinids (Teleostei: Cyprinidae: Danioninae)

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    Rahman, Md. Mizanur; Norén, Michael; Mollah, Abdur Rob

    2017-01-01

    Four species of Devario are recorded from Bangladesh: D. aequipinnatus, D. anomalus, D. coxi, new species, and D. devario. Devario aequipinnatus has a wide distribution in northern India and Bangladesh. Devario coxi, from southeastern Bangladesh near Cox’s Bazar, differs from D. aequipinnatus in mtDNA (COI, p-distance 1.8%), colouration, proportional measurements, and meristics. The minor morphological differences and low frequency of overlapping meristics suggest relatively recent separation of D. coxi from other D. aequipinnatus. Devario anomalus occurs only in southeastern Bangladesh and is here reported from localities in addition to the type locality. It differs from the similar D. xyrops in adjacent Myanmar by slender body shape and by 2.3% p-distance in the COI gene. Specimens of D. anomalus from the Sangu River were found to have the mitochondrial genome of D. aequipinnatus from Bangladesh, but agree with other D. anomalus in the nuclear RAG1 gene. Devario devario has a wide distribution on the Indian Peninsula and border regions; in Bangladesh it is restricted in distribution to the Ganga, Brahmaputra, and Meghna drainages. Reports of D. assamensis and D. malabaricus from Bangladesh are misidentifications. Perilampus ostreographus M’Clelland, 1839, is tentatively synonymized with D. aequipinnatus. Phylogenetic analysis of 14 species of striped devarios based on the COI gene results in a polytomy with four unresolved clades. Devario deruptotalea from the Chindwin basin is the sister group of D. aequipinnatus+D. coxi. Devario devario is the sistergroup of D. xyrops+D. anomalus. PMID:29166410

  19. Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers.

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    Tsigenopoulos, C S; Ráb, P; Naran, D; Berrebi, P

    2002-06-01

    The cyprinid genus Barbus, with more than 800 nominal species, is an apparently polyphyletic assemblage to which a number of unrelated species, groups and/or assemblages have been assigned. It includes species that exhibit three different ploidy levels: diploid, tetraploid and hexaploid. Several lineages of the family Cyprinidae constitute a major component of the African freshwater ichthyofauna, having about 500 species, and fishes assigned to the genus 'Barbus' have the most species on the continent. We used complete sequences of the mitochondrial cytochrome b gene in order to infer phylogenetic relationships between diploid, tetraploid and hexaploid species of 'Barbus' occurring in southern Africa, the only region where representatives of all of the three ploidy levels occur. The results indicate that most of the lineages are incorrectly classified in the genus 'Barbus'. The southern African tetraploids probably originated from southern African diploids. They constitute a monophyletic group distinct from tetraploids occurring in the Euro-Mediterranean region (Barbus sensu stricto). The 'small' African diploid species seem to be paraphyletic, while the 'large' African hexaploid barbs species are of a single, recent origin and form a monophyletic group. The evidence of multiple, independent origins of polyploidy occurring in the African cyprinine cyprinids thus provides a significant contribution to the knowledge on the systematic diversity of these fishes, and warrants a thorough taxonomic reorganization of the genus.

  20. Analysis of mitochondrial DNA: taxonomic and phylogenetic relationships in two fish taxa (Pisces: Mugilidae and Cyprinidae).

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    Semina, A V; Polyakova, N E; Brykov, Vl A

    2007-12-01

    To solve some systematic questions as well as to study genetic variability and evolutionary relationships in two groups of fish belonging to the Mugilid (Mugilidae) and Cyprinid (Cyprinidae) families, we have used restriction fragment length polymorphism analysis of mitochondrial DNA (mtDNA) fragments amplified in polymerase chain reaction. The analysis of three mtDNA fragments of 7220 bp total length of six Mugilid species has shown that Mediterranean Liza aurata, L. ramada, L. saliens, and Chelon labrosus form a common cluster, L. aurata and C. labrosus being the closest relatives, whereas L. haematocheilus (syn. C. haematocheilus) of the Sea of Japan forms a sister group to the Mediterranean cluster. It was found that Chelon and Liza genera are paraphyletic, and therefore their division into two genera is unnatural and they should be synonymized. According to priority, Liza species should be ascribed to Chelon genus. Mugil cephalus is the most distant compared to the rest of the species studied. The level of genetic divergence between allopatric samples of M. cephalus from the Sea of Japan and the Mediterranean Sea has proved to be very high--4.5% of nucleotide substitutions. The analysis of four mtDNA fragments of 9340 bp total length of six Cyprinid species has shown that L. waleckii is the most genetically distant. Pseudaspius leptocephalus is a sister group to Tribolodon species. All Tribolodon species form a common cluster with T. sachalinensis as a root. The remaining species form two branches, one of which includes T. nakamurai and T. brandtii, another one combines T. hakonensis and a new form of Tribolodon revealed that is close to T. hakonensis by its mtDNA (2.4% of nucleotide substitutions). This new form might be an independent species.

  1. CEPF Western Ghats Special Series: Checklist of the fishes of the Achankovil forests, Kerala, India with notes on the range extension of an endemic cyprinid Puntius chalakkudiensis

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    F. Baby

    2011-07-01

    Full Text Available We report the results of an ichthyofaunal inventory carried out in the Achankovil Reserve Forest in the southern Western Ghats as part of a Critical Ecosystem Partnership Fund Project on lesser known freshwater fishes of Kerala . Forty-six species of freshwater fish, belonging to 17 families and 31 genera were collected from 11 sites inside the Achankovil Reserve Forest. Family Cyprinidae dominated with 21 species, followed by Bagridae, Balitoridae and Channidae (three species each. Out of the 46 species, 14 were endemic to the Western Ghats, three were endemic to Kerala region and one was exotic to the country. In this paper, we also report the range extension of an endemic cyprinid, Puntius chalakkudiensis to the Achankovil River and the Achankovil Reserve Forest. The fish diversity of this region is higher than many protected areas within southern Western Ghats, and stresses the need for immediate protection and monitoring programs.

  2. [Research Advances in Cyprinid Herpesvirus 3].

    Science.gov (United States)

    Zheng, Shucheng; Wang, Qing; Li, Yingying; Zeng, Weiwei; Wang, Yingying; Liu, Chun; Liang, Hongru; Shi, Cunbin

    2016-01-01

    Cyprinid herpesvirus 3 (CyHV-3) is the causative agent of an extremely contagious and aggressive disease afflicting common corp Cyprinus carpio L. termed koi herpesvirus disease (KHVD). Since it was first reported in 1997, the virus has spread worldwide rapidly, leading to enormous financial losses in industries based on common carp and koi carp. This review summarizes recent advances in CyHV-3 research on the etiology, epidemiology, pathogenesis, diagnosis, prevention, and control of KHVD.

  3. Remarkable phylogenetic resolution of the most complex clade of Cyprinidae (Teleostei: Cypriniformes): a proof of concept of homology assessment and partitioning sequence data integrated with mixed model Bayesian analyses.

    Science.gov (United States)

    Tao, Wenjing; Mayden, Richard L; He, Shunping

    2013-03-01

    Despite many efforts to resolve evolutionary relationships among major clades of Cyprinidae, some nodes have been especially problematic and remain unresolved. In this study, we employ four nuclear gene fragments (3.3kb) to infer interrelationships of the Cyprinidae. A reconstruction of the phylogenetic relationships within the family using maximum parsimony, maximum likelihood, and Bayesian analyses is presented. Among the taxa within the monophyletic Cyprinidae, Rasborinae is the basal-most lineage; Cyprinine is sister to Leuciscine. The monophyly for the subfamilies Gobioninae, Leuciscinae and Acheilognathinae were resolved with high nodal support. Although our results do not completely resolve relationships within Cyprinidae, this study presents novel and significant findings having major implications for a highly diverse and enigmatic clade of East-Asian cyprinids. Within this monophyletic group five closely-related subgroups are identified. Tinca tinca, one of the most phylogenetically enigmatic genera in the family, is strongly supported as having evolutionary affinities with this East-Asian clade; an established yet remarkable association because of the natural variation in phenotypes and generalized ecological niches occupied by these taxa. Our results clearly argue that the choice of partitioning strategies has significant impacts on the phylogenetic reconstructions, especially when multiple genes are being considered. The most highly partitioned model (partitioned by codon positions within genes) extracts the strongest phylogenetic signals and performs better than any other partitioning schemes supported by the strongest 2Δln Bayes factor. Future studies should include higher levels of taxon sampling and partitioned, model-based analyses. Copyright © 2012 Elsevier Inc. All rights reserved.

  4. Taxonomic review of the genus Luciobarbus Heckel, 1843 (Actinopterygii, Cyprinidae from northwestern Morocco with the description of three new species

    Directory of Open Access Journals (Sweden)

    Casal-Lopez, Miriam

    2015-12-01

    Full Text Available The genus Luciobarbus in Morocco presents high diversification, in contrast to the generally impoverished freshwater fish fauna from North Africa. Within Morocco the northern area is one of the least studied territories, due to both its historical background and the limited accessibility of many regions. Previous phylogenetic studies identified Luciobarbus populations that are morphologically and genetically differentiated, to the same extent as others already recognized as separate species. The aim of this work is to describe these populations as distinct species, based on morphological, meristic, and genetic traits. urn:lsid:zoobank.org:pub:8FC4B423-104C-4097-A468-ED3D2664A15AEn Marruecos el género Luciobarbus está altamente diversificado, en comparación con la empobrecida fauna de peces de agua dulce del norte de África. Dentro de Marruecos la región norte es una de las áreas menos estudiadas, por motivos históricos y también por la poca accesibilidad en algunas de sus regiones. Los estudios filogenéticos previos para esta área han señalado la existencia de poblaciones pertenecientes al género Luciobarbus tan diferenciadas morfológica y genéticamente como otras que ya habían sido reconocidas como diferentes especies. En este trabajo describimos estas poblaciones como diferentes taxa, en base a caracteres morfológicos, merísticos y genéticos.

  5. Taxonomy of rheophilic Luciobarbus Heckel, 1842 (Actinopterygii, Cyprinidae from Morocco with the description of two new species

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    Doadrio, I.

    2016-06-01

    Full Text Available The genus Luciobarbus Heckel, 1843 is characterized by medium to large fish species occurring in large rivers and downstream zones with slow-moving waters. Remarkably also rheophilic Luciobarbus occur in Morocco, which are of small size and exhibit distinct morphological traits as well as different habitat requirements. These rheophilic species have traditionally been assigned to Luciobarbus nasus (Günther, 1874 and L. magniatlantis (Pellegrin, 1919, although some authors consider L. magniatlant as a junior synonym of L. nasus. This lack of consensus on the taxonomy of rheophilic barbs is constrained by limited population studies that do not encompass their entire distribution range. Using molecular, morphometric, and osteological data we studied populations of rheophilic barbs of three drainage basins in which they are currently present: Tensift, Moulouya and Oum er Rbia. Our results clearly identified the rheophilic barbs of each basin as different species. The species Luciobarbus magniatlantis is a well-recognized species endemic to Tensift Basin. In contrast, the investigated populations from the Moulouya and Oum er Rbia basins could not be assigned to any previously described species. Consequently, we describe two new Luciobarbus species in the Moulouya and Oum er Rbia basins.El género Luciobarbus Heckel, 1843 se caracteriza por presentar especies de medio y gran tamaño que viven en las zonas bajas de grandes ríos con aguas lentas. Singularmente, en Marruecos también existen especies reófilas del género Luciobarbus de pequeño tamaño y que presentan diferentes caracteres morfológicos y distintos requerimientos de hábitat. Estas especies reófilas han sido tradicionalmente asignadas a Luciobarbus nasus (Günther, 1874 y L. magniatlantis (Pellegrin, 1919 aunque algunos autores consideran a L. magniatlantis como un sinónimo de L. nasus. Esta falta de consenso en la taxonomía de los barbos reófilos está limitada por los escasos estudios poblacionales realizados que no abarcan la totalidad del área de distribución de estos barbos. Nosotros, usando datos moleculares, morfométricos y osteológicos, estudiamos las poblaciones de los barbos reófilos de tres cuencas hidrográficas, en las cuales están actualmente presentes: Tensift, Moulouya y Oum er Rbia. Nuestros resultados claramente identifican a los barbos reófilos de cada una de estas cuencas como diferentes especies. La especie L. magniatlantis es una especie bien definida y endémica de la cuenca del Tensift. En contraste, las poblaciones analizadas de las cuencas del Moulouya y Oum er Rbia no pudieron ser asignadas a ninguna especie previamente descrita. Consecuentemente, nosotros describimos dos nuevas especies de Luciobarbus en las cuencas del Moulouya y del Oum er Rbia.

  6. Testing freshwater Lago Mare dispersal theory on the phylogeny relationships of iberian cyprinid genera Chondrostoma and Squalius (Cypriniformes, Cyprinidae

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    Carmona, José Ambrosio

    2003-12-01

    Full Text Available A phylogeny of the species in the genera Chondrostoma and Squalius was constructed based on the complete mitochondrial cytochrome b gene (1140pb. The molecular phylogeny was used to test the effect of the Mediterranean Lago Mare dispersal theory on the processes of divergence and speciation of European freshwater fishes. Phylogenetic relationships among Squalius samples and the molecular clock revealed that the ancestor of the current Iberian Squalius species inhabited a wide geographic area in the central and southwestern part of the former Iberian Peninsula during the Miocene before the Lago Mare phase. Similarly, the four main Iberian lineages of the genus Chondrostoma originated in the Middle-Upper Miocene. Hence, the Lago Mare phase of the Mediterranean Sea seems to have been a too recent paleogeographic event to have had any major impact on the dispersion of Squalius and Chondrostoma species. However, the reduction of the water-bodies during the Tortonian and Messinian may have intensified the isolation of populations. The Operational Biogeographic Units recovered from the Squalius and Chondrostoma phylogenies also reject the Lago Mare dispersal theory and support the idea that the differentiation processes were due to both the formation of the current hydrographical basin during the Plio-Pleistocene as well as to an earlier endorrheism event that occurred prior to hydrographical configuration.Realizamos una filogenia de los géneros Chondrostoma y Squalius mediante el estudio de la secuencia completa del gen mitocondrial citocromo b (1140pb. La filogenia molecular fue usada para comprobar el efecto que la teoría de dispersión del Mediterráneo Lago Mare ha tenido sobre los procesos de divergencia y especiación en los peces de agua dulce europeos. Las relaciones filogenéticas entre las muestras de Squalius y la aplicación del reloj molecular pusieron de manifiesto que el ancestro de las actuales especies ibéricas de Squalius habitaba una amplia área del centro y suroeste de la Península Ibérica durante el Mioceno antes de la fase Lago Mare. Del mismo modo, los cuatro grandes linajes ibéricos del género Chondrostoma tuvieron su origen en el Mioceno Medio-Superior. La fase Lago Mare del Mediterráneo parece un hecho demasiado reciente como para haber tenido un impacto importante en la dispersión de las especies de los géneros Squalius y Chondrostoma. No obstante, la reducción de los cuerpos de agua durante el Tortoniense y Mesiniense pudo haber intensificado el aislamiento de las poblaciones. Las Unidades Biogeográficas Operativas obtenidas de las filogenias de los géneros Squalius y Chondrostoma también rechazan la teoría de la dispersión en la fase Lago Mare y sugieren que los procesos de diferenciación de la fauna de peces de agua dulce se debieron al aislamiento de las cuencas hidrográficas, y a fenómenos antiguos de endorreísmo que se produjeron antes de la configuración de las cuencas hidrográficas.

  7. Lake Tana's piscivorous Barbus (Cyprinidae, Ethiopia) ecology - evolution - exploitation

    NARCIS (Netherlands)

    Graaf, de M.

    2003-01-01

    The 15 Barbus species of Lake Tana, a large shallow lake located at an altitude of 1830 m in the north-western highlands of Ethiopia, form the only remaining intact species flock of large (max. 100cm) cyprinid fishes. Lake Tana is the source of the Blue Nile and high waterfalls (40 m) at

  8. Population dynamics and reproductive biology of Barilius bendelisis (Cyprinidae: Cypriniformes) from river Gaula of Central Indian Himalaya.

    Science.gov (United States)

    Saxena, Neha; Patiyal, Rabindar Singh; Dube, Kiran; Tiwari, Virendra K

    2016-09-01

    The Indian hill trout cyprinid, Barilius bendelisis is a member of family Cyprinidae that dwells in shallow, cold, and clear water. In this study, growth parameters and reproductive biology of Indian hill trout, Barilius bendelisis from river Gaula, Central Himalaya region, India, were studied. The length-frequency data were grouped sex wise and were analyzed to determine the growth and mortality parameters using the computer software programme, FAO-ICLARM Stock Assessment Tool (FISAT II). Altogether, 501 individuals were collected from river Gaula (November 2013-October 2014) and were preserved in formalin for further analysis. The results showed that the female outnumbered the male population. The minimum GSI of females was observed in the month of October (4.93 ± 0.26) and for males in the month of June and July (0.093 ± 0.12), whereas, the maximum value was in the month of April for both females (13.47 ± 0.52) and males (1.21 ± 0.12). Fluctuation in GSI values had a bimodal pattern showing two peaks during March-May and August-September in both the sexes, indicating the common spawning period of fish. The slope of regression showed the negative allometric growth for both males and females (b= 2.65 for male and b= 2.5 for female). A significant relationship between length and weight was observed in the present study (p < 0.05). The ELEFAN-I estimated L∞ and K of the von Bertalanffy growth factor for males (17.33 cm and 0.310 per year), females (17.33 cm and 0.3 per year) and pooled sexes (17.33 cm and 0.240 per year). The results indicated that Barilius bendelisis is a small sized fish having negative allometric growth that spawns twice a year. Thus, the present study on biological traits represents the baseline information for effective production, conservation and restoration planning.

  9. The immune system of cyprinid fish = Immunologie van de karper

    NARCIS (Netherlands)

    Rijkers, G.T.

    1980-01-01

    This study deals with several aspects of the immune system of cyprinid fish.

    Some observations on the development of cellular and humoral responsiveness in rosy barb (Barbus conchonius) are described in appendix I. A humoral anti-sheep red blood cell (SRBC) response was demonstrated in

  10. Detection of novel strains of cyprinid herpesvirus closely related to koi herpesvirus

    NARCIS (Netherlands)

    Engelsma, M.Y.; Way, K.; Dodge, M.J.; Voorbergen-Laarman, H.A.; Panzarin, V.M.; Abbadi, M.; El-Matbouli, M.; Skall, H.F.; Kahns, S.; Stone, D.M.

    2013-01-01

    Cyprinid herpesvirus 3 (CyHV-3) or koi herpesvirus (KHV) is a devastating virus of carp. Using generic primers for the DNA polymerase and the major capsid protein genes of cyprinid herpesviruses, nucleotide sequences divergent from previously described CyHV-3 were obtained. At least 3 novel groups

  11. Detection of novel strains of cyprinid herpesvirus closely related to koi herpesvirus

    DEFF Research Database (Denmark)

    Engelsma, Marc Y.; Way, Keith; Dodge, Melanie J.

    2013-01-01

    Cyprinid herpesvirus 3 (CyHV-3) or koi herpesvirus (KHV) is a devastating virus of carp. Using generic primers for the DNA polymerase and the major capsid protein genes of cyprinid herpesviruses, nucleotide sequences divergent from previously described CyHV-3 were obtained. At least 3 novel groups...

  12. Normal axial skeleton structure in common roach Rutilus rutilus (Actinopterygii: Cyprinidae) and malformations due to radiation contamination in the area of the Mayak (Chelyabinsk Province, Russia) nuclear plant.

    Science.gov (United States)

    Bogutskaya, N G; Zuykov, M A; Naseka, A M; Anderson, E B

    2011-10-01

    This study was designed to describe normal axial skeletal structure in common roach Rutilus rutilus from putative unaffected environmental conditions, and the occurrence of skeletal malformations in the fish from an area under radiation contamination. Specimens were collected from water bodies of the Techa Cascade Reservoirs located near the Mayak atomic industry plant in the River Ob' drainage, Chelyabinsk Province, Russia. One sample was collected from Lake Irtyash, a reservoir of drinkable water, supplying the town of Ozersk, and the other one from a technical reservoir which is a storage of liquid radioactive waste from Mayak and characterized by high radioactive contamination (mostly (90)Sr and (137)Cs). A comparison was made with historical material collected from the River Ob' before the middle of the 20th century, i.e. before the environment became affected by radioactive contamination. A high number of abnormalities of the axial skeleton were detected in both Mayak samples, in 94 and 97% of examined specimens, in contrast to about 20% in the historical specimens. The abnormalities were in both the unpaired fins and the vertebral column, including the caudal complex and included supernumerary elements, fusions, deformities and displacement of the elements. Most axial skeleton abnormalities, however, were minor, such as splitting, shortening or deformation of spines. Severe defects, such as extensive scolioses, lordoses and kyphoses, were not found. The causes of the abnormalities were not identified in this study, but the high incidence of malformations may be attributed to genetically determined imbalance during development. The almost equal distribution of abnormalities among the fish from non-contaminated and radioactive contaminated reservoirs may be explained by either recent gene flow within the population of R. rutilus in the River Techa system or the effect of unknown unfavourable environmental factors such as chemical pollution. © 2011 The Authors. Journal of Fish Biology © 2011 The Fisheries Society of the British Isles.

  13. Ecological traits of Squalius lucumonis (Actinopterygii, Cyprinidae and main differences with those of Squalius squalus in the Tiber River Basin (Italy

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    Giannetto D.

    2013-06-01

    Full Text Available Squalius lucumonis (Bianco, 1983 is an endemic species restricted to three river basins in central Italy and listed as endangered according to IUCN Red List. The aim of this research was to increase the information on ecological preferences of this species and to focus on its differences with S. squalus (Bonaparte, 1837. Data collected in 86 different watercourses throughout Tiber River basin were analysed in the research. For each of the 368 river sectors examined, the main environmental parameters and the fish community were considered. The information were analysed by means of the Canonical Correspondence Analysis (CCA while the differences in ecological traits between S. lucumonis and S. squalus were compared by ANOVA. The results of the study showed significant differences in the ecological preferences of the two species: the S. lucumonis showed predilection for smaller watercourses characterised by a lower number of species and a higher degree of integrity of fish community than S. squalus This information allowed to increase the basic knowledge on population biology and ecology of S. lucumonis that could be very useful for the management and conservation of this Italian endemic species.

  14. Comparative Ultrastructure of Langerhans-Like Cells in Spleens of Ray-Finned Fishes (Actinopterygii)

    Czech Academy of Sciences Publication Activity Database

    Lovy, J.; Wright, G. M.; Speare, D. J.; Tyml, Tomáš; Dyková, Iva

    2010-01-01

    Roč. 271, č. 10 (2010), s. 1229-1239 ISSN 0362-2525 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : fish * cyprinidae * halibut * dendritic cells * Langerhans cell * Birbeck granules * ultrastructure Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.773, year: 2010

  15. 'Fish' (Actinopterygii and Elasmobranchii) diversification patterns through deep time.

    Science.gov (United States)

    Guinot, Guillaume; Cavin, Lionel

    2016-11-01

    Actinopterygii (ray-finned fishes) and Elasmobranchii (sharks, skates and rays) represent more than half of today's vertebrate taxic diversity (approximately 33000 species) and form the largest component of vertebrate diversity in extant aquatic ecosystems. Yet, patterns of 'fish' evolutionary history remain insufficiently understood and previous studies generally treated each group independently mainly because of their contrasting fossil record composition and corresponding sampling strategies. Because direct reading of palaeodiversity curves is affected by several biases affecting the fossil record, analytical approaches are needed to correct for these biases. In this review, we propose a comprehensive analysis based on comparison of large data sets related to competing phylogenies (including all Recent and fossil taxa) and the fossil record for both groups during the Mesozoic-Cainozoic interval. This approach provides information on the 'fish' fossil record quality and on the corrected 'fish' deep-time phylogenetic palaeodiversity signals, with special emphasis on diversification events. Because taxonomic information is preserved after analytical treatment, identified palaeodiversity events are considered both quantitatively and qualitatively and put within corresponding palaeoenvironmental and biological settings. Results indicate a better fossil record quality for elasmobranchs due to their microfossil-like fossil distribution and their very low diversity in freshwater systems, whereas freshwater actinopterygians are diverse in this realm with lower preservation potential. Several important diversification events are identified at familial and generic levels for elasmobranchs, and marine and freshwater actinopterygians, namely in the Early-Middle Jurassic (elasmobranchs), Late Jurassic (actinopterygians), Early Cretaceous (elasmobranchs, freshwater actinopterygians), Cenomanian (all groups) and the Paleocene-Eocene interval (all groups), the latter two

  16. Habitat use by 0+ cyprinid fish in the River Great Ouse, East Anglia

    OpenAIRE

    Garner, Paul

    1997-01-01

    This study was designed to examine the habitat use of several species of 0+ cyprinid in the regulated River Great Ouse and to determine the reasons for specific habitat use. In general, all fish species were found associated with the marginal zone, with little diel variation. Use of shallow habitats in the presence of macrophytes correlated well with the distribution of zooplankton in the river channel, the preferred food source of 0+ cyprinids. During the early to late larval phase, all spec...

  17. CyHV-3: the third cyprinid herpesvirus.

    Science.gov (United States)

    Gotesman, Michael; Kattlun, Julia; Bergmann, Sven M; El-Matbouli, Mansour

    2013-07-22

    Common carp (including ornamental koi carp) Cyprinus carpio L. are ecologically and economically important freshwater fish in Europe and Asia. C. carpio have recently been endangered by a third cyprinid herpesvirus, known as cyprinid herpesvirus-3 (CyHV-3), the etiological agent of koi herpesvirus disease (KHVD), which causes significant morbidity and mortality in koi and common carp. Clinical and pathological signs include epidermal abrasions, excess mucus production, necrosis of gill and internal organs, and lethargy. KHVD has decimated major carp populations in Israel, Indonesia, Taiwan, Japan, Germany, Canada, and the USA, and has been listed as a notifiable disease in Germany since 2005, and by the World Organisation for Animal Health since 2007. KHVD is exacerbated in aquaculture because of the relatively high host stocking density, and CyHV-3 may be concentrated by filter-feeding aquatic organisms. CyHV-3 is taxonomically grouped within the family Alloherpesviridae, can be propagated in a number of cell lines, and is active at a temperature range of 15 to 28°C. Three isolates originating from Japan (KHV-J), USA (KHV-U), and Israel (KHV-I) have been sequenced. CyHV-3 has a 295 kb genome with 156 unique open reading frames and replicates in the cell nucleus, and mature viral particles are 170 to 200 nm in diameter. CyHV-3 can be detected by multiple PCR-based methods and by enzyme-linked immunosorbent assay. Several modes of immunization have been developed for KHVD; however, fish immunized with either vaccine or wild-type virus may become carriers for CyHV-3. There is no current treatment for KHVD.

  18. Phylogeny and biogeographic history of the cyprinid fish genus Carassius (Teleostei: Cyprinidae) with focus on natural and anthropogenic arrivals in Europe

    Czech Academy of Sciences Publication Activity Database

    Rylková, K.; Kalous, L.; Bohlen, Jörg; Lamatsch, D.K.; Petrtýl, M.

    2013-01-01

    Roč. 380, č. 383 (2013), s. 13-20 ISSN 0044-8486 R&D Projects: GA ČR GA206/09/1154 Institutional research plan: CEZ:AV0Z5045916 Institutional support: RVO:67985904 Keywords : Carassius auratus complex * Cytochrome b * Distribution Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.828, year: 2013

  19. Are the Immunocompetence and the Presence of Metazoan Parasites in Cyprinid Fish Affected by Reproductive Efforts of Cyprinid Fish?

    Directory of Open Access Journals (Sweden)

    Karolína Rohlenová

    2010-01-01

    Full Text Available Each organism has the limited resources of energy that is distributed among important life traits. A trade-off between immune response and other physiological demands of organism especially costly reproduction is expected. Leuciscus cephalus, the cyprinid fish, was investigated during three periods varying in reproductive investment, that is, before-breeding, breeding, and after-breeding periods. We tested whether a potentially limited investment in immunity during the breeding is associated with higher susceptibility to the metazoan parasites. Following the immunocompetence handicap and sperm protection hypotheses, males expressing more elaborated sexual ornamentation should produce better quality sperm and be more parasitized. We found that reproductive investments in fish play an important role for energy allocation into somatic condition, immunity, and reproduction. The immune parameters including respiratory burst and leukocyte count were higher in breeding; however, parasite species richness and abundance appeared low. Males investing more in spawning tubercles reached high spermatocrite and were more parasitized by digeneans.

  20. Finding of triploid Carassius Gibelio (Bloch, 1780)(Cypriniformes, Cyprinidae), in Turkey

    Czech Academy of Sciences Publication Activity Database

    Kalous, Lukáš; Memis, D.; Bohlen, Jörg

    2004-01-01

    Roč. 28, č. 1 (2004), s. 77-79 ISSN 0399-0974 R&D Projects: GA ČR GA523/02/0460 Institutional research plan: CEZ:AV0Z5045916 Keywords : carassius gibelio * cyprinidae * Turkey Subject RIV: ED - Physiology Impact factor: 0.312, year: 2004

  1. Sahyadria, a new genus of barbs (Teleostei: Cyprinidae from Western Ghats of India

    Directory of Open Access Journals (Sweden)

    R. Raghavan

    2013-11-01

    Full Text Available Redline Torpedo Barbs (Teleostei: Cyprinidae, comprising of two species, Puntius denisonii and P. chalakkudiensis, and six evolutionarily distinct lineages are endemic to the streams of the Western Ghats freshwater ecoregion in peninsular India. Based on molecular and osteological evidence, we demonstrate that these barbs comprise a distinct genus, for which we propose the name Sahyadria.

  2. Phylogeography of Schizopygopsis stoliczkai (Cyprinidae) in Northwest Tibetan Plateau area.

    Science.gov (United States)

    Wanghe, Kunyuan; Tang, Yongtao; Tian, Fei; Feng, Chenguang; Zhang, Renyi; Li, Guogang; Liu, Sijia; Zhao, Kai

    2017-11-01

    Schizopygopsis stoliczkai (Cyprinidae, subfamily Schizothoracinae) is one of the major freshwater fishes endemic to the northwestern margin of the Tibetan Plateau. In the current study, we used mitochondrial DNA markers cytochrome b (Cyt b ) and 16S rRNA (16S), as well as the nuclear marker, the second intron of the nuclear beta-actin gene (Act2), to uncover the phylogeography of S. stoliczkai . In total, we obtained 74 haplotypes from 403 mitochondrial concatenated sequences. The mtDNA markers depict the phylogenetic structures of S. stoliczkai , which consist of clade North and clade South. The split time of the two clades is dated back to 4.27 Mya (95% HPD = 1.96-8.20 Mya). The estimated split time is earlier than the beginning of the ice age of Pleistocene (2.60 Mya), suggesting that the northwestern area of the Tibetan Plateau probably contain at least two glacial refugia for S. stoliczkai . SAMOVA supports the formation of four groups: (i) the Karakash River group; (ii) The Lake Pangong group; (iii) the Shiquan River group; (iv) the Southern Basin group. Clade North included Karakash River, Lake Pangong, and Shiquan River groups, while seven populations of clade South share the haplotypes. Genetic diversity, star-like network, BSP analysis, as well as negative neutrality tests indicate recent expansions events of S. stoliczkai . Conclusively, our results illustrate the phylogeography of S. stoliczkai , implying the Shiquan River is presumably the main refuge for S. stoliczkai .

  3. The lateral line receptor array of cyprinids from different habitats.

    Science.gov (United States)

    Schmitz, Anke; Bleckmann, Horst; Mogdans, Joachim

    2014-04-01

    The lateral line system of teleost fishes consists of an array of superficial and canal neuromasts (CN). Number and distribution of neuromasts and the morphology of the lateral line canals vary across species. We investigated the morphology of the lateral line system in four diurnal European cyprinids, the limnophilic bitterling (Rhodeus sericeus), the indifferent gudgeon (Gobio gobio), and ide (Leuciscus idus), and the rheophilic minnow (Phoxinus phoxinus). All fish had lateral line canals on head and trunk. The total number of both, CN and superficial neuromasts (SN), was comparable in minnow and ide but was greater than in gudgeon and bitterling. The ratio of SNs to CNs for the head was comparable in minnow and bitterling but was greater in gudgeon and ide. The SN-to-CN ratio for the trunk was greatest in bitterling. Polarization of hair cells in CNs was in the direction of the canal. Polarization of hair cells in SNs depended on body area. In cephalic SNs, hair cell polarization was dorso-ventral or rostro-caudal. In trunk SNs, it was rostro-caudal on lateral line scales and dorso-ventral on other trunk scales. On the caudal fin, hair cell polarization was rostro-caudal. The data show that, in the four species studied here, number, distribution, and orientation of CNs and SNs cannot be unequivocally related to habitat. Copyright © 2013 Wiley Periodicals, Inc.

  4. Cyprinid Herpesvirus 3: An Archetype of Fish Alloherpesviruses.

    Science.gov (United States)

    Boutier, Maxime; Ronsmans, Maygane; Rakus, Krzysztof; Jazowiecka-Rakus, Joanna; Vancsok, Catherine; Morvan, Léa; Peñaranda, Ma Michelle D; Stone, David M; Way, Keith; van Beurden, Steven J; Davison, Andrew J; Vanderplasschen, Alain

    2015-01-01

    The order Herpesvirales encompasses viruses that share structural, genetic, and biological properties. However, members of this order infect hosts ranging from molluscs to humans. It is currently divided into three phylogenetically related families. The Alloherpesviridae family contains viruses infecting fish and amphibians. There are 12 alloherpesviruses described to date, 10 of which infect fish. Over the last decade, cyprinid herpesvirus 3 (CyHV-3) infecting common and koi carp has emerged as the archetype of fish alloherpesviruses. Since its first description in the late 1990s, this virus has induced important economic losses in common and koi carp worldwide. It has also had negative environmental implications by affecting wild carp populations. These negative impacts and the importance of the host species have stimulated studies aimed at developing diagnostic and prophylactic tools. Unexpectedly, the data generated by these applied studies have stimulated interest in CyHV-3 as a model for fundamental research. This review intends to provide a complete overview of the knowledge currently available on CyHV-3. © 2015 Elsevier Inc. All rights reserved.

  5. Nationwide Cyprinid herpesvirus 3 contamination in natural rivers of Japan.

    Science.gov (United States)

    Minamoto, Toshifumi; Honjo, Mie N; Yamanaka, Hiroki; Uchii, Kimiko; Kawabata, Zen'ichiro

    2012-08-01

    Cyprinid herpesvirus 3 (CyHV-3) disease is a significant threat for common and koi carp cultivators and for freshwater ecosystems. To determine the prevalence of CyHV-3 in Japanese rivers, a nationwide survey of all national class-A rivers was undertaken in the Summer of 2008. The virus was concentrated from river water samples using the cation-coated filter method. CyHV-3 DNA was detected in 90 rivers, representing 90% of 103 successfully analysed rivers. More than 100,000 copies of CyHV-3 DNA per litre of sample were detected in four rivers, higher than that reported during the Yura River outbreak in 2007. For CyHV-3-positive rivers, the log CyHV-3 density was negatively correlated with the water temperature on the sampling date and positively correlated with the suspended solids and dissolved oxygen, which are annually averaged for each river. Our results demonstrate that virus detection using molecular biology techniques is a powerful tool for monitoring the presence of CyHV-3 in natural environments. Copyright © 2011 Elsevier Ltd. All rights reserved.

  6. Cyprinid fishes of the genus Neolissochilus in Peninsular Malaysia.

    Science.gov (United States)

    Khaironizam, M Z; Akaria-Ismail, M; Armbruster, Jonathan W

    2015-05-22

    Meristic, morphometric and distributional patterns of cyprinid fishes of the genus Neolissochilus found in Peninsular Malaysia are presented. Based on the current concept of Neolissochilus, only two species are present: N. soroides and N. hendersoni. Neolissochilus hendersoni differs from N. soroides by having lower scale and gill raker counts. Neolissochilus soroides has three mouth types (normal with a rounded snout, snout with a truncate edge, and lobe with a comparatively thick lower lip). A PCA of log-transformed measurements did not reveal significant differences between N. hendersoni and N. soroides, or between any of the morphotypes of N. soroides; however, a CVA of log-transformed measurements successfully classified 87.1% of all specimens. Removing body size by running a CVA on all of the principal components except PC1 (which was correlated with length) only slightly decreased the successful classification rate to 86.1%. Differences in morphometrics were as great between the three morphotypes of N. soroides as between any of the morphotypes and N. hendersoni suggesting that the morphotypes should be examined in greater detail with genetic tools. The PCA of morphometrics revealed separate clouds for N. hendersoni and N. soroides, but no differences between the N. soroides morphotypes. This study revealed that N. hendersoni is recorded for the first time in the mainland area of Peninsular Malaysia. Other nominal species of Neolissochilus reported to occur in the river systems of Peninsular Malaysia are discussed. Lissochilus tweediei Herre in Herre & Myers 1937 and Tor soro Bishop 1973 are synonyms of Neolissochilus soroides.

  7. Identification of an essential virulence gene of cyprinid herpesvirus 3.

    Science.gov (United States)

    Boutier, Maxime; Gao, Yuan; Vancsok, Catherine; Suárez, Nicolás M; Davison, Andrew J; Vanderplasschen, Alain

    2017-09-01

    The genus Cyprinivirus consists of a growing list of phylogenetically related viruses, some of which cause severe economic losses to the aquaculture industry. The archetypal member, cyprinid herpesvirus 3 (CyHV-3) causes mass mortalities worldwide in koi and common carp. A CyHV-3 mutant was described previously that is attenuated in vivo by a deletion affecting two genes (ORF56 and ORF57). The relative contributions of ORF56 and ORF57 to the safety and efficacy profile of this vaccine candidate have now been assessed by analysing viruses individually deleted for ORF56 or ORF57. Inoculation of these viruses into carp demonstrated that the absence of ORF56 did not affect virulence, whereas the absence of ORF57 led to an attenuation comparable to, though slightly less than, that of the doubly deleted virus. To demonstrate further the role of ORF57 as a key virulence factor, a mutant retaining the ORF57 region but unable to express the ORF57 protein was produced by inserting multiple in-frame stop codons into the coding region. Analysis of this virus in vivo revealed a safety and efficacy profile comparable to that of the doubly deleted virus. These findings show that ORF57 encodes an essential CyHV-3 virulence factor. They also indicate that ORF57 orthologues in other cypriniviruses may offer promising targets for the rational design of attenuated recombinant vaccines. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  8. First collection of rudd, Scardinius erythrophthalmus (Cyprinidae), in the New River, West Virginia

    Science.gov (United States)

    Easton, R.S.; Orth, D.J.; Burkhead, N.M.

    1993-01-01

    We collected the first rudd, Scardinius erythrophthalmus (Cyprinidae), from the New (Kanawha) River drainage, West Virginia. The rudd has now been reported from 12 states (Arkansas, Kansas, Illinois, Massachusetts, Maine, Missouri, New York, Oklahoma, Virginia, Wisconsin, and West Virginia) and several major river systems. The rapid spread of the rudd has apparently been facilitated by bait dealers. Despite its widespread distribution, there have been no investigations of potential interactions with native aquatic fauna.

  9. The host preference and impact of Argulus japonicus ectoparasite on cyprinids in Central Java, Indonesia

    Science.gov (United States)

    Kismiyati; Wulansari, P. D.; Dewi, N. N.

    2018-04-01

    The most widely cultivated freshwater fish are from Familia Cyprinidae, among others goldfish (Carassius auratus), koi (Cyprinus carpio) and comet goldfish (Carassius auratus auratus). One of the constraints of freshwater fish cultivation is ectoparasite infestation Argulus japonicus. Financial losses have been experienced by some farmers, caused by these ectoparasitic infestaions. This study was aimed to determine the impact of ectoparasite Argulus japonicus infestation on host (freshwater ornamental fish from Familia Cyprinidae), in order to find a preventive solution to treatment on the host. The results showed that prevalence of infested fish by Argulus japonicus were 57 % goldfish, 31 % comet fish and 65 % koi. Changes of histopathology on host were congestion, baoning degeneration, epithelium erosion and inflammatory cell infiltration. The image of infected leukocytes infested by Argulus japonicus were 8.5 % of lymphocytes, 4.7 % neurophils, 3.9 % monocytes, 1.45 % eosinophils and 0,17% basophils.

  10. Spawning migrations of the endemic Labeobarbus (Cyprinidae, Teleostei) species of Lake Tana, Ethiopia, status and threats

    NARCIS (Netherlands)

    Anteneh, W.; Getahun, A.; Dejen, E.; Sibbing, F.A.; Nagelkerke, L.A.J.; de Graaf, M.; Wudneh, T.; Vijverberg, J.; Palstra, A.P.

    2012-01-01

    The reproductive biology of the only known intact species flock of large cyprinids, the 16 Labeobarbus species of Lake Tana (Ethiopia), has been extensively studied for the past two decades. Seven species of Labeobarbus are known to migrate >50 km upstream into tributary rivers for spawning during

  11. Spawning migrations of the endemic Labeobarbus (Cyprinidae, Teleostei) species of Lake Tana, Ethiopia: status and threats

    NARCIS (Netherlands)

    Anteneh, W.; Getahun, A.; Dejen, E.; Sibbing, F.A.; Nagelkerke, L.A.J.; Graaf, de M.; Wudneh, T.; Vijverberg, J.; Palstra, A.P.

    2012-01-01

    The reproductive biology of the only known intact species flock of large cyprinids, the 16 Labeobarbus species of Lake Tana (Ethiopia), has been extensively studied for the past two decades. Seven species of Labeobarbus are known to migrate >50 km upstream into tributary rivers for spawning

  12. A synoptic review of Caryophyllaeus Gmelin, 1790 (Cestoda: Caryophyllidea), parasites of cyprinid fishes

    Czech Academy of Sciences Publication Activity Database

    Barčák, D.; Oros, M.; Hanzelová, V.; Scholz, Tomáš

    2017-01-01

    Roč. 64, AUG 16 (2017), č. článku 027. ISSN 1803-6465 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:60077344 Keywords : tapeworms * freshwater fish * Cyprinidae * systematics * identification key * phylogenetic relationships * Palaearctic Region Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine OBOR OECD: Veterinary science Impact factor: 1.082, year: 2016

  13. Does interspecies hybridization affect the host specificity of parasites in cyprinid fish?

    Czech Academy of Sciences Publication Activity Database

    Šimková, A.; Dávidová, M.; Papoušek, Ivo; Vetešník, Lukáš

    2013-01-01

    Roč. 6, č. 95 (2013), s. 95 ISSN 1756-3305 R&D Projects: GA ČR GAP505/12/0375 Institutional support: RVO:68081766 Keywords : Cyprinid fish * Interspecies hybridization * Metazoan parasites * Monogenea * Host specificity Subject RIV: EG - Zoology Impact factor: 3.251, year: 2013

  14. Dynamics of metal uptake and depuration in a parasitized cyprinid fish (Rastrineobola argentea)

    NARCIS (Netherlands)

    Oyoo-Okoth, E.; Admiraal, W.; Osano, O.; Kraak, M.H.S.; Were-Kogogo, P.J.A.; Gichuki, J.; Ngure, V.; Makwali, J.; Ogwai, C.

    2012-01-01

    Infestation of fish by endoparasites may potentially influence metal uptake and elimination by the host. We quantified the metal uptake rate constant (k(u)) and efflux rate constants (k(e)) of radiolabeled Cd and Co in the cyprinid fish Rastrineobola argentea experimentally infected with the

  15. Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family Herpesviridae.

    Science.gov (United States)

    Waltzek, Thomas B; Kelley, Garry O; Stone, David M; Way, Keith; Hanson, Larry; Fukuda, Hideo; Hirono, Ikuo; Aoki, Takashi; Davison, Andrew J; Hedrick, Ronald P

    2005-06-01

    The sequences of four complete genes were analysed in order to determine the relatedness of koi herpesvirus (KHV) to three fish viruses in the family Herpesviridae: carp pox herpesvirus (Cyprinid herpesvirus 1, CyHV-1), haematopoietic necrosis herpesvirus of goldfish (Cyprinid herpesvirus 2, CyHV-2) and channel catfish virus (Ictalurid herpesvirus 1, IcHV-1). The genes were predicted to encode a helicase, an intercapsomeric triplex protein, the DNA polymerase and the major capsid protein. The results showed that KHV is related closely to CyHV-1 and CyHV-2, and that the three cyprinid viruses are related, albeit more distantly, to IcHV-1. Twelve KHV isolates from four diverse geographical areas yielded identical sequences for a region of the DNA polymerase gene. These findings, with previously published morphological and biological data, indicate that KHV should join the group of related lower-vertebrate viruses in the family Herpesviridae under the formal designation Cyprinid herpesvirus 3 (CyHV-3).

  16. Chromosomes of European cyprinid fishes: comparative cytogenetics and chromosomal characteristics of ribosomal DNAs in nine Iberian chondrostomine species (Leuciscinae)

    Czech Academy of Sciences Publication Activity Database

    Pereira, C. S.; Ráb, Petr; Collares-Pereira, M. J.

    2012-01-01

    Roč. 140, č. 10 (2012), s. 485-495 ISSN 0016-6707 Institutional support: RVO:67985904 Keywords : fish cytotaxonomy * endangered cyprinids * rDNA Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.681, year: 2012

  17. Cross-species amplification of 41 microsatellites in European cyprinids: A tool for evolutionary, population genetics and hybridization studies

    Directory of Open Access Journals (Sweden)

    Gilles André

    2010-05-01

    Full Text Available Abstract Background Cyprinids display the most abundant and widespread species among the European freshwater Teleostei and are known to hybridize quite commonly. Nevertheless, a limited number of markers for conducting comparative differentiation, evolutionary and hybridization dynamics studies are available to date. Findings Five multiplex PCR sets were optimized in order to assay 41 cyprinid-specific polymorphic microsatellite loci (including 10 novel loci isolated from Chondrostoma nasus nasus, Chondrostoma toxostoma toxostoma and Leuciscus leuciscus for 503 individuals (440 purebred specimens and 63 hybrids from 15 European cyprinid species. The level of genetic diversity was assessed in Alburnus alburnus, Alburnoides bipunctatus, C. genei, C. n. nasus, C. soetta, C. t. toxostoma, L. idus, L. leuciscus, Pachychilon pictum, Rutilus rutilus, Squalius cephalus and Telestes souffia. The applicability of the markers was also tested on Abramis brama, Blicca bjoerkna and Scardinius erythrophtalmus specimens. Overall, between 24 and 37 of these markers revealed polymorphic for the investigated species and 23 markers amplified for all the 15 European cyprinid species. Conclusions The developed set of markers demonstrated its performance in discriminating European cyprinid species. Furthermore, it allowed detecting and characterizing hybrid individuals. These microsatellites will therefore be useful to perform comparative evolutionary and population genetics studies dealing with European cyprinids, what is of particular interest in conservation issues and constitutes a tool of choice to conduct hybridization studies.

  18. Selective Herbivory by an Invasive Cyprinid, the Rudd Scardinius erythrophthalmus

    Energy Technology Data Exchange (ETDEWEB)

    Kapuscinski, Kevin L [SUNY-ESF, SUNY College of Environmental Science and Forestry; John, Farrell M [SUNY-ESF, SUNY College of Environmental Science and Forestry; Stehman, Stephen V [SUNY-ESF, SUNY College of Environmental Science and Forestry; Boyer, Gregory L [SUNY-ESF, SUNY College of Environmental Science and Forestry; Fernando, Danilo D [SUNY-ESF, SUNY College of Environmental Science and Forestry; Teece, Mark A [SUNY-ESF, SUNY College of Environmental Science and Forestry; Tschaplinski, Timothy J [ORNL

    2014-01-01

    1. Herbivory by non-native animals is a problem of growing concern globally, especially for ecosystems where significant native herbivores did not previously exist or have been replaced by non-natives. The rudd (Scardinius erythrophthalmus) is an omnivorous cyprinid that has a nearly global longitudinal distribution due to human translocations, yet it is unknown whether the rudd feeds selectively among aquatic macrophyte species common to North American waters. 2. We tested a null hypothesis of non-selective feeding by rudds using five species of aquatic macrophytes: Ceratophyllum demersum, Elodea canadensis, Najas flexilis, Stuckenia pectinata, and Vallisneria americana. Four rudds were placed in 15 different 890-L tanks and presented with known quantities of each macrophyte species (each tank serving as a block in a randomized complete block design). Each macrophyte bundle was weighed on six dates during a 13 d experiment. Differences in mean percent weight remaining among macrophyte species were tested using repeated measures analysis of variance. We also quantified differences among chemical attributes of the five macrophyte species and qualitatively determined if selective feeding by rudds was related to dry matter content (DMC), percent C by dry weight (%C), percent N by dry weight (%N), and the concentrations of total soluble proteins, two organic acids (aconitic and oxalic acid), total soluble phenolic compounds (<1,000 Da), nine soluble phenolic metabolites, and total phenolic compounds. 3. Selective feeding by rudds was evident, with the order of macrophyte removal (from highest to lowest) being: N. flexilis > E. canadensis > S. pectinata > V. americana > C. demersum. Selection was positively related to %C and atomic C:N, but not DMC, %N, or concentration of total soluble proteins, contrary to the expectation that rudds would select the most nutritious plants available. The concentration of aconitic acid was greatest in N. flexilis, a preferred macrophyte

  19. Introgressive hybridization in Iberian cyprinid fishes:a cytogenomic approach to homoploid Leuciscinae

    OpenAIRE

    Pereira, Carla Sofia Alves, 1983-

    2013-01-01

    Tese de doutoramento, Biologia (Biologia Evolutiva), Universidade de Lisboa, Faculdade de Ciências, 2013 Hybridization is currently a well-recognized process amongst animals responsible for biodiversity, evolution and speciation processes while defying most species concepts. Hybridization is prevalent among fishes, particularly cyprinids, which therefore constitute good models of study (1) to access general patterns of genomic variation, (2) to identify the genetic basis and the evolutiona...

  20. Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers

    Czech Academy of Sciences Publication Activity Database

    Tsigenopoulos, C. S.; Ráb, Petr; Naran, D.; Berrebi, P.

    2002-01-01

    Roč. 88, - (2002), s. 466-473 ISSN 0018-067X R&D Projects: GA AV ČR IBS5045011; GA AV ČR KSK6005114 Institutional research plan: CEZ:AV0Z5045916 Keywords : barbus * Cyprinidae * mitochondrial DNA Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.687, year: 2002

  1. Patterns of cyprinid migration through a fishway in relation to light, water temperature and fish circling behaviour

    Czech Academy of Sciences Publication Activity Database

    Prchalová, Marie; Slavík, O.; Bartoš, L.

    2006-01-01

    Roč. 4, č. 3 (2006), s. 213-218 ISSN 1571-5124. [International symposium on Ecohydraulics /5./. Madrid, 12.09.2004-17.09.2004] Institutional research plan: CEZ:AV0Z60170517 Keywords : migration * Cyprinidae * fishway * fish counter * water temperature * weather Subject RIV: EH - Ecology, Behaviour

  2. A comparative biochemical profile of some cyprinids fish in Dukan Lake, Kurdistan-Iraq

    Science.gov (United States)

    Azeez, Darya Mohammed; Mohammed, Sarbaz Ibrahim

    2017-09-01

    The present study was carried out to demonstrate the baseline values for some serum biochemical parameters for 64 adult freshwater fish including seven species belong to family Cyprinidae, have been collected in Dukan Lake, Kurdistan region-Iraq. Fishes were weighed, measured, and collect blood for blood chemistry. Serum biochemical analyses were determined using (Cobas C 311) full automatic chemical analyzer. The result of comparative study of serum biochemical parameters of all Cyprinidae species showed that serum glucose was (459.10±106.99 mg/dl) and direct bilirubin was (0.056±0.021mg/dl) in Barbus grypus, serum total protein (3.511± 0.0484gm/dl) and HDL (133.11±0.4231mg/dl) in Cyprinus carpio, serum cholesterol (338.33±43.923 mg/dl) and LDL (86.11±11.871mg/dl) in Carassius carassius, serum triglyceride (420.0±28.8mg/dl) and ALK (113.93±20.65U/L) in Chondrostoma regium, serum AST and serum ALT in Capoeta trutta, were significantly higher when compared to other species. In a conclusion there is variation in biochemical values among species of same family.

  3. THE EFFECT OF CUTANEOUS SECRETIONS OF CYPRINIDAE FISH ON PATHOGENIC BACTERIA ERYSIPELOTHRIX RHUSIOPATHIAE POPULATIONS

    Directory of Open Access Journals (Sweden)

    O. Gulay

    2014-09-01

    Full Text Available Purpose. To investigate the effect of cutaneous secretions of Cyprinidae fish on the populations of pathogenic bacteria Erysipelothrix rhusiopathiae. Methodology. Pieces of filter paper were placed on the skin of live fish. After a 1 min. exposure, they were removed and placed in glass tubes for the extraction of water-soluble components. Tap water was used as a solvent (previously settled for 48 hours; 0,1 cm3 of water were needed for 1 cm2 area of the filter paper. After extraction, the aqueous solution of fish cutaneous secretions was sterilized by filtering it through filters with pore diameter <0,2 µm. The test was carried out with cultures of E. rhusiopathiae bacteria, which were incubated on heart-brain broth at a temperature of +36,7 ± 0,3 °С for 48-hours. After adding the sterilized tap water and cultures of E. rhusiopathiae bacteria, test samples contained fish cutaneous secretions at following ratios: 1:10, 1:100, 1:1000, 1:10000. As a control, sterilized tap water and E. rhusiopathiae bacteria at ratios similar to test samples were used. In 48 hours, samples were taken from the specimens cultured at a temperature of +18...+20 °С for determination of cell density in E. rhusiopathiae populations. Findings. Aquatic environment, which contains the secretions of skin glands of certain Cyprinidae species, creates favorable conditions for the reproduction and increase in the density of pathogenic E. rhusiopathiae populations. In the conditions of freshwater ecosystems, direct topical biocenotical and trophic relations may be created between pathogenic E. rhusiopathiae bacteria and the studied fish species (рrussian carp Carassius auratus gibelio and wild carp Cyprinus. Originality. For the first time we obtained the quantitative data that demonstrate a stimulating effect of cutaneous secretions of certain fish species on pathogenic E. rhusiopathiae populations. Practical value. The stimulating effect of cutaneous secretions of some

  4. The Significance of Protein Components in Heterogeneous Eggs for Embrionic Development and Larvas Persistence of Cyprinidae

    Directory of Open Access Journals (Sweden)

    Zalepukhin Valeriy Vladimirovich

    2014-12-01

    Full Text Available Endogenous heterogeneity of females and their eggs is one of the most important factors of annual generation’s formation in nature-spawn and incubative centers. Its assessment is significant for comprehending the notion of biochemical changes in proximate prespawn period. Between the shares of egg’s biochemical composition of Cyprinidae species only protein and b-lipoproteids levels are incovering constant and authentic correlations with embrionic development and larval survival in the conditions of artificial reproduction. The promotion of protein’s level to 20 – 24 % (in wet weigth in percolating eggs positively affects the fertilization and larvae vitality. This trend is fair for the domesticated and nature-spawn fish. The same significance is important for the b-lipoproteids level in ovular eggs of grass carp (Ctenopharyngodon idella Val.. The optimal concentration is 100-200 mg % in wet weight.

  5. Description of Rhabdochona (Globochona) rasborae sp. n. (Nematoda: Rhabdochonidae) from the freshwater cyprinid fish Rasbora paviana Tirant in southern Thailand

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Kamchoo, K.

    2012-01-01

    Roč. 59, č. 3 (2012), s. 209-215 ISSN 0015-5683 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : parasitic nematode * Rhabdochona * Globochona * freshwater fish * sidestripe rasbora * Cyprinidae * Thailand Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.515, year: 2012 http://folia.paru.cas.cz/detail.php?id=22068

  6. Coordinated and sequential transcription of the cyprinid herpesvirus-3 annotated genes.

    Science.gov (United States)

    Ilouze, Maya; Dishon, Arnon; Kotler, Moshe

    2012-10-01

    Cyprinid herpesvirus-3 (CyHV-3) is the cause of a fatal disease in carp and koi fish. The disease is seasonal and appears when water temperatures range from 18 to 28°C. CyHV-3 is a member of the Alloherpesviridae, a family in the Herpesvirales order that encompasses mammalian, avian and reptilian viruses. CyHV-3 is a large double-stranded DNA (dsDNA) herpesvirus with a genome of approximately 295kbp, divergent from other mammalian, avian and reptilian herpesviruses, but bearing several genes similar to cyprinid herpesvirus-1 (CyHV-1), CyHV-2, anguillid herpesvirus-1 (AngHV-1), ictalurid herpesvirus-1 (IcHV-1) and ranid herpes virus-1 (RaHV-1). Here we show that viral DNA synthesis commences 4-8h post-infection (p.i.), and is completely inhibited by pre-treatment with cytosine β-d-arabinofuranoside (Ara-C). Transcription of CyHV-3 genes initiates after infection as early as 1-2h p.i., and precedes viral DNA synthesis. All 156 annotated open reading frames (ORFs) of the CyHV-3 genome are transcribed into RNAs, most of which can be classified into immediate early (IE or α), early (E or β) and late (L or γ) classes, similar to all other herpesviruses. Several ORFs belonging to these groups are clustered along the viral genome. Copyright © 2012 Elsevier B.V. All rights reserved.

  7. Development of a PCR assay to detect cyprinid herpesvirus 1 in koi and common carp.

    Science.gov (United States)

    Viadanna, Pedro H O; Miller-Morgan, Tim; Peterson, Trace; Way, Keith; Stone, David M; Marty, Gary D; Pilarski, Fabiana; Hedrick, Ronald P; Waltzek, Thomas B

    2017-02-08

    Cyprinid herpesvirus 1 (CyHV1) infects all scaled and color varieties of common carp Cyprinus carpio, including koi. While it is most often associated with unsightly growths known as 'carp pox,' the underlying lesion (epidermal hyperplasia) can arise from a variety of disease processes. CyHV1-induced epidermal hyperplasia may occur transiently in response to water temperature, and thus histopathology cannot be used in isolation to assess CyHV1 infection status. To address this problem, here we describe a PCR assay targeted to the putative thymidine kinase gene of CyHV1. The PCR assay generates a 141 bp amplicon and reliably detects down to 10 copies of control plasmid DNA sequence (analytic sensitivity). The PCR does not cross-detect genomic DNA from cyprinid herpesvirus 2 and 3 (analytic specificity). The CyHV1 PCR effectively detected viral DNA in koi and common carp sampled from various locations in the UK, USA, Brazil, and Japan. Viral DNA was detected in both normal appearing and grossly affected epidermal tissues from koi experiencing natural epizootics. The new CyHV1 PCR provides an additional approach to histopathology for the rapid detection of CyHV1. Analysis of the thymidine kinase gene sequences determined for 7 PCR-positive carp originating from disparate geographical regions identified 3 sequence types, with 1 type occurring in both koi and common carp.

  8. Molecular characterisation and prevalence of a new genotype of Cyprinid herpesvirus 2 in mainland China.

    Science.gov (United States)

    Li, Lijuan; Luo, Yangzhi; Gao, Zexia; Huang, Jian; Zheng, Xianghai; Nie, Huihui; Zhang, Junmei; Lin, Li; Yuan, Junfa

    2015-06-01

    Cyprinid herpesvirus 2 (CyHV-2, species Cyprinid herpesvirus 2) has been confirmed as a causative agent of the acute haematopoietic necrosis disease outbreak in farmed goldfish (Carassius auratus L.) and gibel carp (Carassius auratus gibelio Bloch). In this study, we present the genomic characteristics of a variant CyHV-2 strain (SY-C1) isolated from farmed gibel carp in mainland China and its comparative genomics analysis with the CyHV-2 reference strain ST-J1. Overall, the full-length genome of SY-C1 shares 98.8% homology with that of ST-J1. Sequence comparisons between SY-C1 and ST-J1 indicate that the variations include single-nucleotide mutations, insertions, deletions, and rearrangements, which suggested that SY-C1 is different from ST-J1 and represents a new genotype. Therefore, we propose that the identified CyHV-2 can be divided into 2 different genotypes and be named China genotype (C genotype) and Japan genotype (J genotype) according to their isolation loci. Furthermore, epidemiological surveys indicate that the dominant genotype of CyHV-2 circulating in mainland China is closer to the China genotype than the Japan genotype.

  9. Rational development of an attenuated recombinant cyprinid herpesvirus 3 vaccine using prokaryotic mutagenesis and in vivo bioluminescent imaging

    Science.gov (United States)

    Cyprinid herpesvirus 3 (CyHV-3) is causing severe economic losses worldwide in the carp industry, and a safe and efficacious attenuated vaccine compatible with mass vaccination is needed. We produced single deleted recombinants using prokaryotic mutagenesis. When producing a recombinant lacking open...

  10. Detection of Cyprinid herpesvirus 2 in association with an Aeromonas sobria infection of Carassius carassius (L.), in Italy

    DEFF Research Database (Denmark)

    Fichi, G.; Cardeti, G.; Cocumelli, C.

    2013-01-01

    herpesvirus‐like particles, subsequently identified as Cyprinid herpesvirus‐2 (CyHV‐2) with a nested PCR protocol. Although it was not possible to attribute a pathogenic role to CyHV‐2 in this mortality event, the identification of this herpesvirus in crucian carp increases the concern about its potential...

  11. A molecular approach to the genus Alburnoides using COI sequences data set and the description of a new species, A. damghani, from the Damghan River system (the Dasht-e Kavir Basin, Iran) (Actinopterygii, Cyprinidae).

    Science.gov (United States)

    Roudbar, Arash Jouladeh; Eagderi, Soheil; Esmaeili, Hamid Reza; Coad, Brian W; Bogutskaya, Nina

    2016-01-01

    The molecular status of nine species of the genus Alburnoides from different river drainages in Iran and additionally by seven species from Europe was assessed. mtDNA COI gene sequences from freshly collected specimens and available NCBI data revealed four major phylogenetic lineages. Based on the results, a distinct taxon from the Cheshmeh Ali (Ali Spring), a Damghan River tributary in the endorheic Dasht-e Kavir basin, northern Iran, which is the closest sister to Alburnoides namaki (Namak Lake basin) + Alburnoides coadi (Nam River in the endorheic Dasht-e Kavir basin) is considered as a new species, Alburnoides damghani sp. n. It is distinguished from other Alburnoides species in Iran by a combination of character states including: a weakly-developed, variably-scaled, ventral keel from completely scaleless to completely scaled, a short snout with the tip of the mouth cleft on a level with the lower margin of the pupil or slightly lower, a small eye (eye horizontal diameter slightly to markedly less than interorbital width), commonly 8½ branched dorsal-fin rays, commonly 11-12½ branched anal-fin rays, 40-46(47) total lateral-line scales, 2.5-4.2 or 2.5-4.1 pharyngeal teeth, gill rakers short and widely spaced, 6-8 in total, 39-41 (commonly 40), total vertebrae, (19)20(21) abdominal vertebrae, 19-21 (most commonly 20) caudal vertebrae, abdominal vertebral region most commonly equal to or longer than caudal region, and most common vertebral formulae 20+20 and 21+19.

  12. A molecular approach to the genus Alburnoides using COI sequences data set and the description of a new species, A. damghani, from the Damghan River system (the Dasht-e Kavir Basin, Iran) (Actinopterygii, Cyprinidae)

    OpenAIRE

    Jouladeh Roudbar,Arash; Eagderi,Soheil; Esmaeili,Hamid Reza; Coad,Brian; Bogutskaya,Nina

    2016-01-01

    Abstract The molecular status of nine species of the genus Alburnoides from different river drainages in Iran and additionally by seven species from Europe was assessed. mtDNA COI gene sequences from freshly collected specimens and available NCBI data revealed four major phylogenetic lineages. Based on the results, a distinct taxon from the Cheshmeh Ali (Ali Spring), a Damghan River tributary in the endorheic Dasht-e Kavir basin, northern Iran, which is the closest sister to Alburnoides namak...

  13. Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates

    Directory of Open Access Journals (Sweden)

    Se Chang Park

    2013-01-01

    Full Text Available Cyprinid herpes virus 3 (CyHV-3 diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio. Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116 of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116. In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA, Israel, Japan isolates. These findings may be suitable for many applications including isolates differentiation and phylogeny studies.

  14. Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates

    Science.gov (United States)

    Han, Jee Eun; Kim, Ji Hyung; Renault, Tristan; Choresca, Casiano; Shin, Sang Phil; Jun, Jin Woo; Park, Se Chang

    2013-01-01

    Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA, Israel, Japan isolates. These findings may be suitable for many applications including isolates differentiation and phylogeny studies. PMID:23435236

  15. Investigation of latent infections caused by cyprinid herpesvirus 3 in koi ( Cyprinus carpio) in southern China.

    Science.gov (United States)

    Zheng, Shucheng; Wang, Qing; Bergmann, Sven M; Li, Yingying; Zeng, Weiwei; Wang, Yingying; Liu, Chun; Shi, Cunbin

    2017-05-01

    Although herpesviruses such as cyprinid herpesvirus 3 (CyHV-3) can establish lifelong latent infections, little is known about latency conditions in farmed koi populations in China. We used nested polymerase chain reaction targeting the TK gene and an indirect antibody ELISA to screen asymptomatic fish obtained from southern China for evidence of CyHV-3 infection. CyHV-3 DNA could be detected either in peripheral blood leukocytes or from gills of asymptomatic koi. Most koi sera did not contain anti-CyHV-3 antibodies; however, 5 samples were ELISA positive, providing evidence of prior CyHV-3 infections. These findings suggest that koi may survive CyHV-3 infections and become virus carriers.

  16. Cyprinid herpesvirus 3: an interesting virus for applied and fundamental research

    Science.gov (United States)

    2013-01-01

    Cyprinid herpesvirus 3 (CyHV-3), a member of the family Alloherpesviridae is the causative agent of a lethal, highly contagious and notifiable disease in common and koi carp. The economic importance of common and koi carp industries together with the rapid spread of CyHV-3 worldwide, explain why this virus became soon after its isolation in the 1990s a subject of applied research. In addition to its economic importance, an increasing number of fundamental studies demonstrated that CyHV-3 is an original and interesting subject for fundamental research. In this review, we summarized recent advances in CyHV-3 research with a special interest for studies related to host-virus interactions. PMID:24073814

  17. Barcoding and border biosecurity: identifying cyprinid fishes in the aquarium trade.

    Directory of Open Access Journals (Sweden)

    Rupert A Collins

    Full Text Available Poorly regulated international trade in ornamental fishes poses risks to both biodiversity and economic activity via invasive alien species and exotic pathogens. Border security officials need robust tools to confirm identifications, often requiring hard-to-obtain taxonomic literature and expertise. DNA barcoding offers a potentially attractive tool for quarantine inspection, but has yet to be scrutinised for aquarium fishes. Here, we present a barcoding approach for ornamental cyprinid fishes by: (1 expanding current barcode reference libraries; (2 assessing barcode congruence with morphological identifications under numerous scenarios (e.g. inclusion of GenBank data, presence of singleton species, choice of analytical method; and (3 providing supplementary information to identify difficult species.We sampled 172 ornamental cyprinid fish species from the international trade, and provide data for 91 species currently unrepresented in reference libraries (GenBank/Bold. DNA barcodes were found to be highly congruent with our morphological assignments, achieving success rates of 90-99%, depending on the method used (neighbour-joining monophyly, bootstrap, nearest neighbour, GMYC, percent threshold. Inclusion of data from GenBank (additional 157 spp. resulted in a more comprehensive library, but at a cost to success rate due to the increased number of singleton species. In addition to DNA barcodes, our study also provides supporting data in the form of specimen images, morphological characters, taxonomic bibliography, preserved vouchers, and nuclear rhodopsin sequences. Using this nuclear rhodopsin data we also uncovered evidence of interspecific hybridisation, and highlighted unrecognised diversity within popular aquarium species, including the endangered Indian barb Puntius denisonii.We demonstrate that DNA barcoding provides a highly effective biosecurity tool for rapidly identifying ornamental fishes. In cases where DNA barcodes are unable to

  18. TELEOSTEI; CYPRINIDAE

    African Journals Online (AJOL)

    these fISh (Love 1970), but the effects of maintaining fISh in laboratory aquaria on their haematology per se have not yet been investigated and laboratory data are usually thought to apply to wild fish as well. Newly caught, exhausted fish cannot be used for the determination of certain physiological parameters (Love 1970) ...

  19. Phenotypic differentiation of Barilius bendelisis (Cypriniformes: Cyprinidae) in four rivers from Central Indian Himalaya.

    Science.gov (United States)

    Mir, Javaid Iqbal; Saxena, Neha; Patiyal, Rabindar Singh; Sahoo, Prabhati Kumari

    2015-03-01

    Barilius bendelisis, commonly known as Indian Hill Trout is an upland water fish of South East Asia. It belongs to the family Cyprinidae and dwells in shallow, clear and cold water. In this study, the intraspecific variation of Barilius bendelisis, on the basis of morphometric characters, was investigated. Altogether, 402 specimens were collected from four rivers in the Central Indian Himalaya. A truss network was constructed by interconnecting 12 landmarks to yield 30 distance variables that were extracted from digital images of specimens using tpsDig2 and PAST software. Allometric transformed truss measurements were subjected to univariate analysis of variance, factor analysis and discriminant analysis. All variables exhibited significant differences between the populations. Altogether 88% of the specimens were classified into their original populations (81.98% under a 'leave-one-out' procedure). With factor analysis measurements of the head region, the middle portion and the caudal region had high loadings on the first and second axis. The results indicated that B. bendelisis has significant phenotypic heterogeneity between the geographically isolated regions of Central Indian Himalaya. We hypothesize that the marked interspecific variation in B. bendelisis is the result of local ecological conditions.

  20. Biological studies of Cyprinid Fish, Barbus luteus (Heckel) fromAl-Hammar Marsh in Southern Iraq

    International Nuclear Information System (INIS)

    Nasir, N.A.

    2006-01-01

    This study was carried out over a period of one year to investigate someaspects of the biology of Barbus luteus (Pisces: Cyprinidae) in the Al-Hammarmarsh area of Iraq. The fish are most abundant between February and June,followed by a steady decrease towards the end of the year. A mean monthlymortality rate of 35% per month was recorded. Total length and weight of 1002fish collected ranged between 120-320mm (mean: 174mm) and 21-275 gm (Mean:79gm) respectively. Numerically, the samples were dominated by females. Theyaccounted for about 52.7% of the total catch. The population of B. luteus wasdominated by 2+ year-olds for both sexes and they matured at this age. Themathematical relationship between the weight and length of the fish wasdetermined. The values of the exponent (b) showed that females are generallyheavier at a given length than males. The food taken by the fish was studiedand it was found that fish is principally a surface phytoplankton feeder.(author)

  1. Biogeography and pattern of gene flow among Barbus species (Teleostei:Cyprinidae) inhabiting the Italian Peninsula and neighbouring Adriatic drainages as revealed by allozyme and mitochondrial sequence data

    Czech Academy of Sciences Publication Activity Database

    Tsigenopoulos, C. S.; Kotlík, Petr; Berrebi, P.

    2002-01-01

    Roč. 75, - (2002), s. 83-99 ISSN 0024-4066 R&D Projects: GA AV ČR IBS5045111; GA AV ČR KSK6005114 Keywords : allozymes * Barbus * Cyprinidae Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.705, year: 2002

  2. A new species of parasitic copepod, Sarcotretes umitakae sp. n. (Siphonostomatoida, Pennellidae, on the rattail (Actinopterygii, Macrouridae from the East China Sea, Japan

    Directory of Open Access Journals (Sweden)

    Daisuke Uyeno

    2012-11-01

    Full Text Available A new species of copepod, Sarcotretes umitakae sp. n., of the siphonostomatoid family Pennellidae is described based on female specimens from the rattail Coelorinchus jordani Smith and Pope (Actinopterygii: Gadiformes: Macrouridae caught in the East China Sea. This species is characterized by exhibiting the following characters: the large proboscis projects strongly; the head bears paired lateral processes which are bulbous and taper into a slender horn; the twisting neck is significantly longer than the trunk; and the trunk bears an anterior constriction with a reduced abdomen.

  3. Two species of Rhabdochona Railliet, 1916 (Nematoda: Rhabdochonidae) parasitising cyprinid fishes in Iraq, with a redescription of R. tigridis Rahemo, 1978 (emend.)

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Saraiva, A.; Abdullah, S. M. A.; Bilal, S. J.; Rahemo, Z. I. F.

    2009-01-01

    Roč. 74, č. 2 (2009), s. 125-135 ISSN 0165-5752 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Rhabdochona * Cyprinidae * Iraq Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.911, year: 2009

  4. Does hardness of food affect the development of pharyngeal teeth of the black carp, Mylopharyngodon piceus (Pisces: Cyprinidae)?

    DEFF Research Database (Denmark)

    Hung, Nguyen Manh; Ryan, Timothy M.; Stauffer, Jay R.

    2015-01-01

    We investigated whether food type influences development of the pharyngeal crushing apparatus of black carp (Mylopharyngodon piceus, Cyprinidae). Fish fed a hard diet had average standard lengths and weights larger than those fed a soft diet; these observations in part could be related to differe......We investigated whether food type influences development of the pharyngeal crushing apparatus of black carp (Mylopharyngodon piceus, Cyprinidae). Fish fed a hard diet had average standard lengths and weights larger than those fed a soft diet; these observations in part could be related...... to differences in nutritional value of the two food types. The lower pharyngeal bones, which bear molariform teeth were examined using three dimensional computed tomography (CT) scans. After adjusting for differences in the standard length of the fish, the total volume and exterior surface areas...... of the pharyngeal teeth were greater in fish fed hard diets than in those fed soft diets. Total weights of the pharyngeal arches were less in the fish fed a soft diet than in those fed a hard diet. These results indicated that food type affects development of the pharyngeal crushing mill of black carp and therefore...

  5. Boom and bust: ancient and recent diversification in bichirs (Polypteridae: Actinopterygii), a relictual lineage of ray-finned fishes.

    Science.gov (United States)

    Near, Thomas J; Dornburg, Alex; Tokita, Masayoshi; Suzuki, Dai; Brandley, Matthew C; Friedman, Matt

    2014-04-01

    Understanding the history that underlies patterns of species richness across the Tree of Life requires an investigation of the mechanisms that not only generate young species-rich clades, but also those that maintain species-poor lineages over long stretches of evolutionary time. However, diversification dynamics that underlie ancient species-poor lineages are often hidden due to a lack of fossil evidence. Using information from the fossil record and time calibrated molecular phylogenies, we investigate the history of lineage diversification in Polypteridae, which is the sister lineage of all other ray-finned fishes (Actinopterygii). Despite originating at least 390 million years (Myr) ago, molecular timetrees support a Neogene origin for the living polypterid species. Our analyses demonstrate polypterids are exceptionally species depauperate with a stem lineage duration that exceeds 380 million years (Ma) and is significantly longer than the stem lineage durations observed in other ray-finned fish lineages. Analyses of the fossil record show an early Late Cretaceous (100.5-83.6 Ma) peak in polypterid genus richness, followed by 60 Ma of low richness. The Neogene species radiation and evidence for high-diversity intervals in the geological past suggest a "boom and bust" pattern of diversification that contrasts with common perceptions of relative evolutionary stasis in so-called "living fossils." © 2013 The Author(s). Evolution © 2013 The Society for the Study of Evolution.

  6. Identification of B cells as a major site for cyprinid herpesvirus 3 latency.

    Science.gov (United States)

    Reed, Aimee N; Izume, Satoko; Dolan, Brian P; LaPatra, Scott; Kent, Michael; Dong, Jing; Jin, Ling

    2014-08-01

    Cyprinid herpesvirus 3 (CyHV-3), commonly known as koi herpesvirus (KHV), is a member of the Alloherpesviridae, and is a recently discovered emerging herpesvirus that is highly pathogenic for koi and common carp. Our previous study demonstrated that CyHV-3 becomes latent in peripheral white blood cells (WBC). In this study, CyHV-3 latency was further investigated in IgM(+) WBC. The presence of the CyHV-3 genome in IgM(+) WBC was about 20-fold greater than in IgM(-) WBC. To determine whether CyHV-3 expressed genes during latency, transcription from all eight open reading frames (ORFs) in the terminal repeat was investigated in IgM(+) WBC from koi with latent CyHV-3 infection. Only a spliced ORF6 transcript was found to be abundantly expressed in IgM(+) WBC from CyHV-3 latently infected koi. The spliced ORF6 transcript was also detected in vitro during productive infection as early as 1 day postinfection. The ORF6 transcript from in vitro infection begins at -127 bp upstream of the ATG codon and ends +188 bp downstream of the stop codon, +20 bp downstream of the polyadenylation signal. The hypothetical protein of ORF6 contains a consensus sequence with homology to a conserved domain of EBNA-3B and ICP4 from Epstein-Barr virus and herpes simplex virus 1, respectively, both members of the Herpesviridae. This is the first report of latent CyHV-3 in B cells and identification of gene transcription during latency for a member of the Alloherpesviridae. This is the first demonstration that a member of the Alloherpesviridae, cyprinid herpesvirus 3 (CyHV-3), establishes a latent infection in the B cells of its host, Cyprinus carpio. In addition, this is the first report of identification of gene transcription during latency for a member of Herpesvirales outside Herpesviridae. This is also the first report that the hypothetical protein of latent transcript of CyHV-3 contains a consensus sequence with homology to a conserved domain of EBNA-3B from Epstein-Barr virus and ICP4

  7. A RAD-based linkage map and comparative genomics in the gudgeons (genus Gnathopogon, Cyprinidae

    Directory of Open Access Journals (Sweden)

    Kakioka Ryo

    2013-01-01

    Full Text Available Abstract Background The construction of linkage maps is a first step in exploring the genetic basis for adaptive phenotypic divergence in closely related species by quantitative trait locus (QTL analysis. Linkage maps are also useful for comparative genomics in non-model organisms. Advances in genomics technologies make it more feasible than ever to study the genetics of adaptation in natural populations. Restriction-site associated DNA (RAD sequencing in next-generation sequencers facilitates the development of many genetic markers and genotyping. We aimed to construct a linkage map of the gudgeons of the genus Gnathopogon (Cyprinidae for comparative genomics with the zebrafish Danio rerio (a member of the same family as gudgeons and for the future QTL analysis of the genetic architecture underlying adaptive phenotypic evolution of Gnathopogon. Results We constructed the first genetic linkage map of Gnathopogon using a 198 F2 interspecific cross between two closely related species in Japan: river-dwelling Gnathopogon elongatus and lake-dwelling Gnathopogon caerulescens. Based on 1,622 RAD-tag markers, a linkage map spanning 1,390.9 cM with 25 linkage groups and an average marker interval of 0.87 cM was constructed. We also identified a region involving female-specific transmission ratio distortion (TRD. Synteny and collinearity were extensively conserved between Gnathopogon and zebrafish. Conclusions The dense SNP-based linkage map presented here provides a basis for future QTL analysis. It will also be useful for transferring genomic information from a “traditional” model fish species, zebrafish, to screen candidate genes underlying ecologically important traits of the gudgeons.

  8. Effects of Malathion Acute Toxicity on Behavioral and Haematological Parameters in Capoeta damascina (Cypriniformes: Cyprinidae

    Directory of Open Access Journals (Sweden)

    Saeed Shahbazi

    2015-07-01

    Full Text Available   The present study investigated the effects of acute toxicity of malathion on behavioral and haematological parameters in the cyprinid Capoeta damascina. The specimens were collected from the Kordan River, Karaj, Iran in August, 2014 and were exposed to different concentrations of malathion at the laboratory (24, 48, 72 mg L‑1 based on 96h-LC (10; 30; 50; 90 which was 6.08 (5.22-7.18 mg L-1. The animals were then exposed to 0.76, 1.00 and 1.52 mg L-1 of malathion for 10 days. Blood samples were collected in days 1, 5 and 10. Red blood cell (RBC, white blood cell (WBC, hemoglobin (Hb, hematocrit (PVC, mean corpuscular volume (MCV, mean corpuscular hemoglobin (MCH, and mean corpuscular hemoglobin concentration (MCHC were measured. Behavioral abnormalities were observed in fishes exposed to high levels of malathion. The specimens exposed to malathion had significantly lower RBC, WBC, Hb, PVC but higher MCV and MCH than those of the control group. No significant difference was detected in MCHC the exposed and control specimens. Hematological parameters (except MCHC were significantly correlated with exposure time. In conclusion, malathion showed extensive haematological effects on C. damascina that might be used as bioindicator of this pesticide in flowing waters.  

  9. Combined effects of hydrologic alteration and cyprinid fish in mediating biogeochemical processes in a Mediterranean stream.

    Science.gov (United States)

    Rubio-Gracia, Francesc; Almeida, David; Bonet, Berta; Casals, Frederic; Espinosa, Carmen; Flecker, Alexander S; García-Berthou, Emili; Martí, Eugènia; Tuulaikhuu, Baigal-Amar; Vila-Gispert, Anna; Zamora, Lluis; Guasch, Helena

    2017-12-01

    Flow regimes are important drivers of both stream community and biogeochemical processes. However, the interplay between community and biogeochemical responses under different flow regimes in streams is less understood. In this study, we investigated the structural and functional responses of periphyton and macroinvertebrates to different densities of the Mediterranean barbel (Barbus meridionalis, Cyprinidae) in two stream reaches differing in flow regime. The study was conducted in Llémena Stream, a small calcareous Mediterranean stream with high nutrient levels. We selected a reach with permanent flow (permanent reach) and another subjected to flow regulation (regulated reach) with periods of flow intermittency. At each reach, we used in situ cages to generate 3 levels of fish density. Cages with 10 barbels were used to simulate high fish density (>7indm -2 ); cages with open sides were used as controls (i.e. exposed to actual fish densities of each stream reach) thus having low fish density; and those with no fish were used to simulate the disappearance of fish that occurs with stream drying. Differences in fish density did not cause significant changes in periphyton biomass and macroinvertebrate density. However, phosphate uptake by periphyton was enhanced in treatments lacking fish in the regulated reach with intermittent flow but not in the permanent reach, suggesting that hydrologic alteration hampers the ability of biotic communities to compensate for the absence of fish. This study indicates that fish density can mediate the effects of anthropogenic alterations such as flow intermittence derived from hydrologic regulation on stream benthic communities and associated biogeochemical processes, at least in eutrophic streams. Copyright © 2017. Published by Elsevier B.V.

  10. Characteristics of cyprinid herpesvirus 3 in different phases of infection: implications for disease transmission and control.

    Science.gov (United States)

    Sunarto, Agus; McColl, Kenneth A; Crane, Mark St J; Schat, Karel A; Slobedman, Barry; Barnes, Andrew C; Walker, Peter J

    2014-08-08

    Koi herpesvirus disease (KHVD) is an emerging and highly contagious viral disease of koi and common carp (Cyprinus carpio), causing mass mortalities and huge economic losses to the carp aquaculture industry. The disease has spread rapidly to 28 countries worldwide. However, mechanisms of koi herpesvirus (species Cyprinid herpesvirus 3; CyHV-3) transmission remain unclear. A potential experimental model of CyHV-3 infection in carp was used to characterise CyHV-3 in different phases of infection and to demonstrate that CyHV-3 persists in survivor fish and has the capacity to reactivate and transmit the disease to healthy fish. During acute infection, which occurred when fish were maintained at 22°C, viral genes were abundantly expressed and infectious virus was produced in association with tissue damage, clinical disease and mortality. In fish maintained at a lower temperature (11°C), viral DNA was present but viral gene expression was absent or greatly restricted, infectious virus was not recovered and there was no evidence of disease. Productive replication was re-initiated following an increase in water temperature to 22°C, resulting in 45% mortality. Shedding of reactivated virus killed 75% of cohabitating naïve fish, suggesting a potential risk for disease transmission. Crown Copyright © 2014. Published by Elsevier B.V. All rights reserved.

  11. Cyprinid herpesvirus 2 infection emerged in cultured gibel carp, Carassius auratus gibelio in China.

    Science.gov (United States)

    Xu, Jin; Zeng, Lingbing; Zhang, Hui; Zhou, Yong; Ma, Jie; Fan, Yuding

    2013-09-27

    An epizootic with severe mortality has emerged in cultured gibel carp, Carassius auratus gibelio, in China since 2009, and caused huge economic loss. The signs and epidemiology background of the disease were investigated. Parasite examination, bacteria and virus isolation were carried out for pathogen isolation. The causative pathogen was obtained and identified as Cyprinid herpesvirus 2 (CyHV-2) by experimental infection, electron microscopy, cell culture, PCR assay and sequence alignment, designated as CyHV-2-JSSY. Experimental infection proved the high virulence of CyHV-2-JSSY to healthy gibel carp. Electron microscopy revealed that the viral nucleocapsid was hexagonal in shape measuring 110-120 nm in diameter with a 170-200 nm envelope. The virus caused significant CPE in Koi-Fin cells at the early passages, but not beyond the fifth passages. Sequence alignment of the partial viral helicase gene (JX566884) showed that it shared 99-100% identity to the published sequences of other CyHV-2 isolates. This study represented the first isolation and identification of CyHV-2 in cultured gibel carp in China and laid a foundation for the further studies of the disease. Copyright © 2013 Elsevier B.V. All rights reserved.

  12. Interdemic variation in the African cyprinid Barbus neumayeri: correlations among hypoxia, morphology, and feeding performance

    International Nuclear Information System (INIS)

    Schaack, S.; Chapman, L.J.

    2003-01-01

    Adaptive change in one character may affect associated, but functionally unrelated, characters in adaptive or nonadaptive ways. For example, adaptive change in gill size (i.e., larger gills in hypoxic habitats) may alter adjacent feeding structures and therefore have indirect effects on feeding behavior. This study examines whether differences in total gill filament length among populations of the African cyprinid Barbus neumayeri correlate with variation in trophic structures and feeding performance. Morphological measurements revealed larger total gill filament length in populations from low-oxygen habitats. Differences in external and internal morphology of the trophic apparatus correlated with those found in gills and suggest a reduction in food capture and processing ability in fish with larger respiratory apparatus. In paired feeding trials on a novel prey type, large-gilled fish spent more time feeding than small-gilled fish of the same body size without ingesting more food. This suggests less efficient food uptake in large-gilled fish from hypoxic habitats. We suggest that variation in gill size facilitates the broad habitat distribution of B. neumayeri but may limit the success of phenotypes crossing physicochemical gradients. (author)

  13. Molecular phylogeny of the tribe Torini Karaman, 1971 (Actinopterygii: Cypriniformes) from the Middle East and North Africa.

    Science.gov (United States)

    Borkenhagen, Kai

    2017-02-22

    Freshwater fishes of the cyprinid tribe Torini are widespread in Africa the Middle East and Indomalaya. The relationships of Middle-Eastern Torini are analysed based on mitochondrial markers (Cyt b, ND4) of the majority of relevant species. I present a larely well resolved phylogeny, which confirms the validity of the morphologically defined genera Arabibarbus, Carasobarbus, Mesopotamichthys and Pterocapoeta. The Torini originated in Indomalaya and colonised Africa via the Middle East. Morocco was colonised two times independently, first from sub-Saharan Africa and secondly along the southern margin of the Mediterranean Sea. The Tigris-Euphrates system is an important crossroad for the colonisation of the Jordan River, the Orontes River and the watercourses of the Arabian Peninsula by freshwater fishes. The Jordan lost its connection to the Euphrates earlier than the Orontes. The Arabian Peninsula was colonised from the Tigris-Euphrates system in at least two independent events.

  14. Efeito da densidade de estocagem do quinguio, Carassius auratus L., 1758 (Osteichthyes, Cyprinidae, em suas fases iniciais de desenvolvimento Stocking density effect on goldfish, Carassius auratus L., 1758 (Osteichthyes, Cyprinidae fry, during its initial development phases

    Directory of Open Access Journals (Sweden)

    Christiano Rodrigues Schamber

    2002-05-01

    Full Text Available Objetivou-se avaliar a influência da densidade de estocagem sobre o desenvolvimento do quinguio, Carassius auratus (Osteichthyes, Cyprinidae em suas fases iniciais. Foram utilizados 300 larvas (Lt = 7,00 mm, distribuídas em 20 aquários (12-L. O delineamento experimental utilizado foi o inteiramente casualizado com quatro tratamentos (0,50; 1,00; 1,50 e 2,00 larvas/L e cinco repetições. A alimentação utilizada foi à base de náuplios de Artemia sp. (fornecida de forma proporcional a densidade e ração com 40% de proteína bruta (fornecida à vontade. Observou-se aumento linear (p0,001 pelas diferentes densidades de estocagem utilizadas. Os valores dos parâmetros físico-químicos permaneceram nos níveis adequados para o cultivo de peixes, embora os valores de pH tenham apresentado redução linear (pThis experiment aimed to evaluate the influence of stocking density on the goldfish, Carassius auratus (Osteichthyes, Cyprinidae development, during its initial phases. 300 fry (Lt.: 7.00 mm were distributed in 20 12-liter aquariums. The experimental design was entirely randomized, with four treatments (0.50; 1.00; 1.50 and 2.00 fry/liter and five replications. Food consisted of Artemia sp nauplii (supplied proportionally to density and rations with 40% raw protein, given ad libitum. While the biomass per aquarium linear increase (p0.001. On the other hand, quadract effect (p 0.001 by the different stocking densities. The physical-chemical parameters rates remained at adequate levels for fish culture, although pH values suffered linear decrease (pCarassius auratus fry culture can use such density during its initial development phases.

  15. Proteomic and Functional Analyses of the Virion Transmembrane Proteome of Cyprinid Herpesvirus 3.

    Science.gov (United States)

    Vancsok, Catherine; Peñaranda, M Michelle D; Raj, V Stalin; Leroy, Baptiste; Jazowiecka-Rakus, Joanna; Boutier, Maxime; Gao, Yuan; Wilkie, Gavin S; Suárez, Nicolás M; Wattiez, Ruddy; Gillet, Laurent; Davison, Andrew J; Vanderplasschen, Alain F C

    2017-11-01

    Virion transmembrane proteins (VTPs) mediate key functions in the herpesvirus infectious cycle. Cyprinid herpesvirus 3 (CyHV-3) is the archetype of fish alloherpesviruses. The present study was devoted to CyHV-3 VTPs. Using mass spectrometry approaches, we identified 16 VTPs of the CyHV-3 FL strain. Mutagenesis experiments demonstrated that eight of these proteins are essential for viral growth in vitro (open reading frame 32 [ORF32], ORF59, ORF81, ORF83, ORF99, ORF106, ORF115, and ORF131), and eight are nonessential (ORF25, ORF64, ORF65, ORF108, ORF132, ORF136, ORF148, and ORF149). Among the nonessential proteins, deletion of ORF25, ORF132, ORF136, ORF148, or ORF149 affects viral replication in vitro , and deletion of ORF25, ORF64, ORF108, ORF132, or ORF149 impacts plaque size. Lack of ORF148 or ORF25 causes attenuation in vivo to a minor or major extent, respectively. The safety and efficacy of a virus lacking ORF25 were compared to those of a previously described vaccine candidate deleted for ORF56 and ORF57 (Δ56-57). Using quantitative PCR, we demonstrated that the ORF25 deleted virus infects fish through skin infection and then spreads to internal organs as reported previously for the wild-type parental virus and the Δ56-57 virus. However, compared to the parental wild-type virus, the replication of the ORF25-deleted virus was reduced in intensity and duration to levels similar to those observed for the Δ56-57 virus. Vaccination of fish with a virus lacking ORF25 was safe but had low efficacy at the doses tested. This characterization of the virion transmembrane proteome of CyHV-3 provides a firm basis for further research on alloherpesvirus VTPs. IMPORTANCE Virion transmembrane proteins play key roles in the biology of herpesviruses. Cyprinid herpesvirus 3 (CyHV-3) is the archetype of fish alloherpesviruses and the causative agent of major economic losses in common and koi carp worldwide. In this study of the virion transmembrane proteome of CyHV-3, the

  16. Phylogenetic relationships of hexaploid large-sized barbs (genus Labeobarbus, Cyprinidae) based on mtDNA data.

    Science.gov (United States)

    Tsigenopoulos, Costas S; Kasapidis, Panagiotis; Berrebi, Patrick

    2010-08-01

    The phylogenetic relationships among species of the Labeobarbus genus (Teleostei, Cyprinidae) which comprises large body-sized hexaploid taxa were inferred using complete cytochrome b mitochondrial gene sequences. Molecular data suggest two main evolutionary groups which roughly correspond to a Northern (Middle East and Northwest Africa) and a sub-Saharan lineage. The splitting of the African hexaploids from their Asian ancestors and their subsequent diversification on the African continent occurred in the Late Miocene, a period in which other cyprinins also invaded Africa and radiated in the Mediterranean region. Finally, systematic implications of these results to the taxonomic validity of genera or subgenera such as Varicorhinus, Kosswigobarbus, Carasobarbus and Capoeta are further discussed. Copyright 2010 Elsevier Inc. All rights reserved.

  17. Skin mucus of Cyprinus carpio inhibits cyprinid herpesvirus 3 binding to epidermal cells

    Directory of Open Access Journals (Sweden)

    Raj Victor

    2011-08-01

    Full Text Available Abstract Cyprinid herpesvirus 3 (CyHV-3 is the aetiological agent of a mortal and highly contagious disease in common and koi carp. The skin is the major portal of entry of CyHV-3 in carp after immersion in water containing the virus. In the present study, we used in vivo bioluminescence imaging to investigate the effect of skin mucus removal and skin epidermis lesion on CyHV-3 entry. Physical treatments inducing removal of the mucus up to complete erosion of the epidermis were applied on a defined area of carp skin just before inoculation by immersion in infectious water. CyHV-3 entry in carp was drastically enhanced on the area of the skin where the mucus was removed with or without associated epidermal lesion. To investigate whether skin mucus inhibits CyHV-3 binding to epidermal cells, tail fins with an intact mucus layer or without mucus were inoculated ex vivo. While electron microscopy examination revealed numerous viral particles bound on the fins inoculated after mucus removal, no particle could be detected after infection of mucus-covered fins. Finally, anti-CyHV-3 neutralising activity of mucus extract was tested in vitro. Incubation of CyHV-3 with mucus extract reduced its infectivity in a dose dependent manner. The present study demonstrates that skin mucus removal and epidermal lesions enhance CyHV-3 entry in carp. It highlights the role of fish skin mucus as an innate immune protection against viral epidermal entry.

  18. Skin mucus of Cyprinus carpio inhibits cyprinid herpesvirus 3 binding to epidermal cells

    Science.gov (United States)

    2011-01-01

    Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of a mortal and highly contagious disease in common and koi carp. The skin is the major portal of entry of CyHV-3 in carp after immersion in water containing the virus. In the present study, we used in vivo bioluminescence imaging to investigate the effect of skin mucus removal and skin epidermis lesion on CyHV-3 entry. Physical treatments inducing removal of the mucus up to complete erosion of the epidermis were applied on a defined area of carp skin just before inoculation by immersion in infectious water. CyHV-3 entry in carp was drastically enhanced on the area of the skin where the mucus was removed with or without associated epidermal lesion. To investigate whether skin mucus inhibits CyHV-3 binding to epidermal cells, tail fins with an intact mucus layer or without mucus were inoculated ex vivo. While electron microscopy examination revealed numerous viral particles bound on the fins inoculated after mucus removal, no particle could be detected after infection of mucus-covered fins. Finally, anti-CyHV-3 neutralising activity of mucus extract was tested in vitro. Incubation of CyHV-3 with mucus extract reduced its infectivity in a dose dependent manner. The present study demonstrates that skin mucus removal and epidermal lesions enhance CyHV-3 entry in carp. It highlights the role of fish skin mucus as an innate immune protection against viral epidermal entry. PMID:21816061

  19. VARIOUS FACTORS AFFECTING DRESSING PERCENTAGE OF COMMERCIALLY CULTURED CYPRINID FISH IN CARP FISH PONDS IN SERBIA

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    Todor Marković

    2012-12-01

    Full Text Available The aim of this study was to determine the yield carcass of all categories of cyprinid fish reared in ponds in Serbia. Samples of two and three-year old carp, two-year old silver carp and grass carp were taken in the winter from a pond where the production is organized in a semi­intensive system. The three­year old carp was sampled from two ponds. In one case, it fed on barley, maize and wheat in the following proportions 40:30:30, while in the second case it fed on complete diet mixtures. Also, the samples of two­year old carp were taken from ponds where they fed on complete feed mixture. Dressing percentage was the most favourable in common carp (67%, followed by silver carp (62%, and it was the least in grass carp (60% (p<0.01. The best yield (66% was obtained in two-year old carp, followed by one-year old carp (64%, and the worst yield was determined in three-year old carp (58% (p<0.01. Carcasses yield was better in two-year old carp fed on pelleted feed (68% than in carp of the same age fed on grains (66% (p<0.01. The values of dressing percentage measured in three-year old carp reared in the semi-intensive system was 56%, and 59% in three-year old carp fed on pelleted complete feed mixture. Fish species, age, system of husbandry and diet showed a significant effect on carcasses yield. The highest dressing percentage and weight of fillets was noted in two-year old carp fed on complete feed, and it was a result of lower weight of internal organs and associated fat. The obtained results may be helpful in creating the best strategy for the selection of raw fish for fish manufacturing.

  20. Diagnostic validation of three test methods for detection of cyprinid herpesvirus 3 (CyHV-3).

    Science.gov (United States)

    Clouthier, Sharon C; McClure, Carol; Schroeder, Tamara; Desai, Megan; Hawley, Laura; Khatkar, Sunita; Lindsay, Melissa; Lowe, Geoff; Richard, Jon; Anderson, Eric D

    2017-03-06

    Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of koi herpesvirus disease in koi and common carp. The disease is notifiable to the World Organisation for Animal Health. Three tests-quantitative polymerase chain reaction (qPCR), conventional PCR (cPCR) and virus isolation by cell culture (VI)-were validated to assess their fitness as diagnostic tools for detection of CyHV-3. Test performance metrics of diagnostic accuracy were sensitivity (DSe) and specificity (DSp). Repeatability and reproducibility were measured to assess diagnostic precision. Estimates of test accuracy, in the absence of a gold standard reference test, were generated using latent class models. Test samples originated from wild common carp naturally exposed to CyHV-3 or domesticated koi either virus free or experimentally infected with the virus. Three laboratories in Canada participated in the precision study. Moderate to high repeatability (81 to 99%) and reproducibility (72 to 97%) were observed for the qPCR and cPCR tests. The lack of agreement observed between some of the PCR test pair results was attributed to cross-contamination of samples with CyHV-3 nucleic acid. Accuracy estimates for the PCR tests were 99% for DSe and 93% for DSp. Poor precision was observed for the VI test (4 to 95%). Accuracy estimates for VI/qPCR were 90% for DSe and 88% for DSp. Collectively, the results show that the CyHV-3 qPCR test is a suitable tool for surveillance, presumptive diagnosis and certification of individuals or populations as CyHV-3 free.

  1. Susceptibility of Japanese Cyprininae fish species to cyprinid herpesvirus 2 (CyHV-2).

    Science.gov (United States)

    Ito, Takafumi; Maeno, Yukio

    2014-03-14

    Cyprinid herpesvirus 2 (CyHV-2) is known as the causative agent of herpesviral haematopoietic necrosis (HVHN) of goldfish (Carassius auratus). Recently, the virus has also been detected from Prussian carp (C. gibelio) and crucian carp (C. carassius) from European and Asian countries. To analyze the risk of spreading to new host species, the susceptibility of other fish species to the virus is essential. In this study experimental infections of indigenous Cyprininae species in Japan were performed by immersion in and intraperitoneal injection of a CyHV-2 isolate. Although Edonishiki, a variety of goldfish, immersed with the virus showed a cumulative mortality of 90%, no mortality was observed in ginbuna C. auratus langsdorfii, nagabuna C. auratus buergeri, nigorobuna C. auratus grandoculis and common carp Cyprinus carpio. Cumulative mortality was 100, 20 and 10% in intraperitoneally injected Edonishiki, ginbuna and nagabuna, respectively. Furthermore all Edonishiki immersed with the virus died. However, even after stimuli of sudden temperature changes, the immersed ginbuna and nagabuna did not die. Moreover no mortality was observed in co-reared Ranchu, another variety of goldfish, with immersed ginbuna and nagabuna although all three Ranchu co-reared with immersed Edonishiki died. CyHV-2 DNA was detected and the virus was re-isolated from all dead fish. Moreover CyHV-2 DNA was detected from some of the surviving Carassius spp. These results revealed that susceptibility of Japanese indigenous Cyprininae fish species to CyHV-2 is much lower than for goldfish. In addition, ability of replication of CyHV-2 might be different among Carassius fish species. Copyright © 2014 Elsevier B.V. All rights reserved.

  2. Khawia abbottinae sp n. (Cestoda: Caryophyllidea) from the Chinese false gudgeon Abbottina rivularis (Cyprinidae: Gobioninae) in China: morphological and molecular data

    Czech Academy of Sciences Publication Activity Database

    Xi, B. W.; Oros, M.; Wang, G. T.; Scholz, Tomáš; Xie, J.

    2013-01-01

    Roč. 60, č. 2 (2013), s. 141-148 ISSN 0015-5683 R&D Projects: GA ČR GA524/08/0885; GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Cestoda * Khawia * Abbottina * Cyprinidae * Yangtze River * 18S rDNA * ITS1 Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.211, year: 2013

  3. The effect of hybridization on fish physiology, immunity and blood biochemistry: A case study in hybridizing Cyprinus carpio and Carassius gibelio (Cyprinidae)

    Czech Academy of Sciences Publication Activity Database

    Šimková, A.; Vojtek, L.; Halačka, Karel; Hyršl, P.; Vetešník, Lukáš

    2015-01-01

    Roč. 435, č. 1 (2015), s. 381-389 ISSN 0044-8486 R&D Projects: GA ČR GAP505/12/0375 Institutional support: RVO:68081766 Keywords : Hybridization * Cyprinids * Blood biochemistry * Hematology * Immunity Subject RIV: EG - Zoology Impact factor: 1.893, year: 2015

  4. Rapid detection of Cyprinid herpesvirus-3 (CyHV-3) using a gold nanoparticle-based hybridization assay.

    Science.gov (United States)

    Saleh, Mona; El-Matbouli, Mansour

    2015-06-01

    Cyprinid herpesvirus-3 (CyHV-3) is a highly infectious pathogen that causes fatal disease in common and koi carp Cyprinus carpio L. CyHV-3 detection is usually based on virus propagation or amplification of the viral DNA using the PCR or LAMP techniques. However, due to the limited susceptibility of cells used for propagation, it is not always possible to successfully isolate CyHV-3 even from tissue samples that have high virus titres. All previously described detection methods including PCR-based assays are time consuming, laborious and require specialized equipment. To overcome these limitations, gold nanoparticles (AuNPs) have been explored for direct and sensitive detection of DNA. In this study, a label-free colorimetric nanodiagnostic method for direct detection of unamplified CyHV-3 DNA using gold nanoparticles is introduced. Under appropriate conditions, DNA probes hybridize with their complementary target sequences in the sample DNA, which results in aggregation of the gold nanoparticles and a concomitant colour change from red to blue, whereas test samples with non complementary DNA sequences remain red. In this study, gold nanoparticles were used to develop and evaluate a specific and sensitive hybridization assay for direct and rapid detection of the highly infectious pathogen termed Cyprinid herpesvirus-3. Copyright © 2015 Elsevier B.V. All rights reserved.

  5. Multi-Omics Analysis Reveals a Correlation between the Host Phylogeny, Gut Microbiota and Metabolite Profiles in Cyprinid Fishes

    Science.gov (United States)

    Li, Tongtong; Long, Meng; Li, Huan; Gatesoupe, François-Joël; Zhang, Xujie; Zhang, Qianqian; Feng, Dongyue; Li, Aihua

    2017-01-01

    Gut microbiota play key roles in host nutrition and metabolism. However, little is known about the relationship between host genetics, gut microbiota and metabolic profiles. Here, we used high-throughput sequencing and gas chromatography/mass spectrometry approaches to characterize the microbiota composition and the metabolite profiles in the gut of five cyprinid fish species with three different feeding habits raised under identical husbandry conditions. Our results showed that host species and feeding habits significantly affect not only gut microbiota composition but also metabolite profiles (ANOSIM, p ≤ 0.05). Mantel test demonstrated that host phylogeny, gut microbiota, and metabolite profiles were significantly related to each other (p ≤ 0.05). Additionally, the carps with the same feeding habits had more similarity in gut microbiota composition and metabolite profiles. Various metabolites were correlated positively with bacterial taxa involved in food degradation. Our results shed new light on the microbiome and metabolite profiles in the gut content of cyprinid fishes, and highlighted the correlations between host genotype, fish gut microbiome and putative functions, and gut metabolite profiles. PMID:28367147

  6. Patterns of parasite distribution in the hybrids of non-congeneric cyprinid fish species: is asymmetry in parasite infection the result of limited coadaptation?

    Czech Academy of Sciences Publication Activity Database

    Krasnovyd, V.; Vetešník, Lukáš; Gettová, L.; Civáňová, K.; Šimková, A.

    2017-01-01

    Roč. 47, č. 8 (2017), s. 471-483 ISSN 0020-7519 R&D Projects: GA ČR GAP505/12/0375 Institutional support: RVO:68081766 Keywords : Interspecific hybrids * Cyprinid fish * Parasite communities * Host specificity * Maternal ancestry Subject RIV: EG - Zoology OBOR OECD: Parasitology Impact factor: 3.730, year: 2016

  7. Identification and Characterization of Cyprinid Herpesvirus-3 (CyHV-3 Encoded MicroRNAs.

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    Owen H Donohoe

    Full Text Available MicroRNAs (miRNAs are a class of small non-coding RNAs involved in post-transcriptional gene regulation. Some viruses encode their own miRNAs and these are increasingly being recognized as important modulators of viral and host gene expression. Cyprinid herpesvirus 3 (CyHV-3 is a highly pathogenic agent that causes acute mass mortalities in carp (Cyprinus carpio carpio and koi (Cyprinus carpio koi worldwide. Here, bioinformatic analyses of the CyHV-3 genome suggested the presence of non-conserved precursor miRNA (pre-miRNA genes. Deep sequencing of small RNA fractions prepared from in vitro CyHV-3 infections led to the identification of potential miRNAs and miRNA-offset RNAs (moRNAs derived from some bioinformatically predicted pre-miRNAs. DNA microarray hybridization analysis, Northern blotting and stem-loop RT-qPCR were then used to definitively confirm that CyHV-3 expresses two pre-miRNAs during infection in vitro. The evidence also suggested the presence of an additional four high-probability and two putative viral pre-miRNAs. MiRNAs from the two confirmed pre-miRNAs were also detected in gill tissue from CyHV-3-infected carp. We also present evidence that one confirmed miRNA can regulate the expression of a putative CyHV-3-encoded dUTPase. Candidate homologues of some CyHV-3 pre-miRNAs were identified in CyHV-1 and CyHV-2. This is the first report of miRNA and moRNA genes encoded by members of the Alloherpesviridae family, a group distantly related to the Herpesviridae family. The discovery of these novel CyHV-3 genes may help further our understanding of the biology of this economically important virus and their encoded miRNAs may have potential as biomarkers for the diagnosis of latent CyHV-3.

  8. Identification and Characterization of Cyprinid Herpesvirus-3 (CyHV-3) Encoded MicroRNAs

    Science.gov (United States)

    Donohoe, Owen H.; Henshilwood, Kathy; Way, Keith; Hakimjavadi, Roya; Stone, David M.; Walls, Dermot

    2015-01-01

    MicroRNAs (miRNAs) are a class of small non-coding RNAs involved in post-transcriptional gene regulation. Some viruses encode their own miRNAs and these are increasingly being recognized as important modulators of viral and host gene expression. Cyprinid herpesvirus 3 (CyHV-3) is a highly pathogenic agent that causes acute mass mortalities in carp (Cyprinus carpio carpio) and koi (Cyprinus carpio koi) worldwide. Here, bioinformatic analyses of the CyHV-3 genome suggested the presence of non-conserved precursor miRNA (pre-miRNA) genes. Deep sequencing of small RNA fractions prepared from in vitro CyHV-3 infections led to the identification of potential miRNAs and miRNA–offset RNAs (moRNAs) derived from some bioinformatically predicted pre-miRNAs. DNA microarray hybridization analysis, Northern blotting and stem-loop RT-qPCR were then used to definitively confirm that CyHV-3 expresses two pre-miRNAs during infection in vitro. The evidence also suggested the presence of an additional four high-probability and two putative viral pre-miRNAs. MiRNAs from the two confirmed pre-miRNAs were also detected in gill tissue from CyHV-3-infected carp. We also present evidence that one confirmed miRNA can regulate the expression of a putative CyHV-3-encoded dUTPase. Candidate homologues of some CyHV-3 pre-miRNAs were identified in CyHV-1 and CyHV-2. This is the first report of miRNA and moRNA genes encoded by members of the Alloherpesviridae family, a group distantly related to the Herpesviridae family. The discovery of these novel CyHV-3 genes may help further our understanding of the biology of this economically important virus and their encoded miRNAs may have potential as biomarkers for the diagnosis of latent CyHV-3. PMID:25928140

  9. [Ontogenetic Mechanisms of Explosive Morphological Divergence in the Lake Tana (Ethiopia) Species Flock of Large African Barbs (Labeobarbus; Cyprinidae; Teleostei)].

    Science.gov (United States)

    Shkila, F N; Lazebny, O E; Kapitanova, D V; Abdissa, Belay; Borisov, V B; Smirnov, S V

    2015-01-01

    Species flock of Lake Tana (Ethiopia) large African barbs (Labeobarbus; Cyprinidae; Teleostei) was studied as a model system for investigating ontogenetic mechanisms of the explosive morphological divergence often accompanying sympatric speciation in bony fishes. Comparative morphological analysis carried out with the use ofgeometric morphometric techniques revealed quantitative differences in the head shapes of species under study. Comparative analysis of skull development revealed significant interspecies differences in the temporal characteristics of craniogenesis in these species. These two lines of evidence suggest that heterochronies in craniogenesis underlie divergence in the head shapes of adult Tana barbs. This prediction was verified via experimental changes of temporal characteristics of craniogenesis in L. intermedius, a putative ancestor for the Lake Tana species flock. For this aim, timing and rate of skull development were changed by artificial manipulation of thyroid hormone levels. In sum, it was shown that it is heterochronies that underlie an explosive morphological divergence of the Lake Tana barbs species flock. Our findings together with those reported in the literature suggest variability in the activity of the hypothalamic-pituitary-thyroid axis to contribute to these heterochronies.

  10. Biologie de la reproduction du Cyprinidae, Barbus callensis dans le lac de barrage Hamiz (Algérie

    Directory of Open Access Journals (Sweden)

    Ould Rouis, S.

    2012-01-01

    Full Text Available Reproductive Biology of the Cyprinidae, Barbus callensis in an Algerian Dam. The freshwater fish, Barbus callensis (Valenciennes, 1842 was studied from 2005 to 2007 for the first time in Hamiz dam. This species endemic of North African continent is widely spread in the rivers of Algeria. This reservoir is situated in Boumerdes 35 km southeast of Algiers. During these three years, the monthly sampling was carried out by trammel nets, fishing near the dike during one night. At the same time, the fry was captured with a transparent plastic bottle baited with breadcrumbs put down near the lakeshore. The monthly follow-up of the gonadosomatic ratio (RGS reveals that the spawning period occurred between April and May. The evolution of the condition factor (K shows low seasonal variations. However, a lower value appears in spring when the temperature increases and the breeding begins. The sex ratio is higher for the adult females (1:2.8, more than 26 cm. The first sexual maturity size (L50 is lower for males (19.6 cm than for females (27.7 cm.

  11. Mitochondrial phylogeny of Chinese barred species of the cyprinid genus Acrossocheilus Oshima, 1919 (Teleostei: Cypriniformes) and its taxonomic implications.

    Science.gov (United States)

    Yuan, Le-Yang; Liu, Xiao-Xiang; Zhang, E

    2015-12-21

    Sequences from the mitochondrial control region of 14 putative species of Acrossocheilus (Cyprinidae) were examined to elucidate phylogenetic relationships within species of the barred group in that genus. Phylogenetic reconstructions were generated using three tree-building methods: maximum parsimony, maximum likelihood, and Bayesian inference. The resultant phylogenies were consistent with monophyly of the majority of the morphologically recognized species. However, mitochondrial DNA sequence evidence is incongruent with monophyly of A. fasciatus, as currently conceived. This species occurs only in the upper Qiantang-Jiang basin in Zhejiang and Anhui provinces, and coastal rivers in the Zhejiang Province. The species formerly recognized as A. paradoxus from Zhejiang Province is A. fasciatus. The specimens previously reported as A. fasciatus from river basins in Fujian Province are misidentified A. wuyiensis. The barred group of Acrossocheilus is shown to be polyphyletic. Acrossocheilus is restricted to the barred species here placed in "Clade II," containing A. paradoxus and relatives. Separate generic status is recommended for A. monticola and for A. longipinnis and their closest relatives, although more information on phylogenetic relationships based on multiple genes is required to develop robust phylogenetic hypotheses and diagnoses. Masticbarbus Tang, 1942 is available for A. longipinnis and three allied species (A. iridescens, A. microstomus and A. lamus).

  12. Cyprininae phylogeny revealed independent origins of the Tibetan Plateau endemic polyploid cyprinids and their diversifications related to the Neogene uplift of the plateau.

    Science.gov (United States)

    Wang, Xuzhen; Gan, Xiaoni; Li, Junbing; Chen, Yiyu; He, Shunping

    2016-11-01

    Origin and diversification of the Tibetan polyploid cyprinids (schizothoracins) may help us to explore relationships between diversification of the cyprinids and the Tibetan Plateau uplift. Cyprininae phylogeny was analyzed using mitochondrial and nuclear DNA sequences to trace origins of polyploidy and diversifications of schizothoracins. Ancestral states reconstruction for ploidy levels indicated that the Cyprininae was diploid origin and the schizothoracin clades tetraploid origins. There were two diversification rate shifts along with diversification of the cyprinine fishes in response to the Tibetan uplift. The unusual diversification shifts were located to branches subtending the clades of Tibetan polyploid cyprinids. Our analyses suggested that (i) phylogeny of Cyprininae recovered two independent origins of the Tibetan polyploidy schizothoracins; (ii) diversifications of the schizothoracins were closely related to the Neogene uplift of the Tibetan plateau in the following ways: the relatively ancient Late Oligocene-Middle Miocene adaptive radiation may be associated with the uplift of the southern Tibet and Himalaya; the Middle Miocene-Early Pleistocene lineage-specific diversification broadly coincident with major phase of the Neogene Tibetan uplift; and the most recent Pleistocene diversification shift in Schizothorax closely coincident with the successive Kunlun-Huanghe and Gonghe movements of the Tibetan uplift and the glaciation-induced climate oscillations on the plateau.

  13. Phylogeny and biogeography of highly diverged freshwater fish species (Leuciscinae, Cyprinidae, Teleostei) inferred from mitochondrial genome analysis.

    Science.gov (United States)

    Imoto, Junichi M; Saitoh, Kenji; Sasaki, Takeshi; Yonezawa, Takahiro; Adachi, Jun; Kartavtsev, Yuri P; Miya, Masaki; Nishida, Mutsumi; Hanzawa, Naoto

    2013-02-10

    The distribution of freshwater taxa is a good biogeographic model to study pattern and process of vicariance and dispersal. The subfamily Leuciscinae (Cyprinidae, Teleostei) consists of many species distributed widely in Eurasia and North America. Leuciscinae have been divided into two phyletic groups, leuciscin and phoxinin. The phylogenetic relationships between major clades within the subfamily are poorly understood, largely because of the overwhelming diversity of the group. The origin of the Far Eastern phoxinin is an interesting question regarding the evolutionary history of Leuciscinae. Here we present phylogenetic analysis of 31 species of Leuciscinae and outgroups based on complete mitochondrial genome sequences to clarify the phylogenetic relationships and to infer the evolutionary history of the subfamily. Phylogenetic analysis suggests that the Far Eastern phoxinin species comprised the monophyletic clades Tribolodon, Pseudaspius, Oreoleuciscus and Far Eastern Phoxinus. The Far Eastern phoxinin clade was independent of other Leuciscinae lineages and was closer to North American phoxinins than European leuciscins. All of our analysis also suggested that leuciscins and phoxinins each constituted monophyletic groups. Divergence time estimation suggested that Leuciscinae species diverged from outgroups such as Tincinae to be 83.3 million years ago (Mya) in the Late Cretaceous and leuciscin and phoxinin shared a common ancestor 70.7 Mya. Radiation of Leuciscinae lineages occurred during the Late Cretaceous to Paleocene. This period also witnessed the radiation of tetrapods. Reconstruction of ancestral areas indicates Leuciscinae species originated within Europe. Leuciscin species evolved in Europe and the ancestor of phoxinin was distributed in North America. The Far Eastern phoxinins would have dispersed from North America to Far East across the Beringia land bridge. The present study suggests important roles for the continental rearrangements during the

  14. DNA barcoding and evaluation of genetic diversity in Cyprinidae fish in the midstream of the Yangtze River.

    Science.gov (United States)

    Shen, Yanjun; Guan, Lihong; Wang, Dengqiang; Gan, Xiaoni

    2016-05-01

    The Yangtze River is the longest river in China and is divided into upstream and mid-downstream regions by the Three Gorges (the natural barriers of the Yangtze River), resulting in a complex distribution of fish. Dramatic changes to habitat environments may ultimately threaten fish survival; thus, it is necessary to evaluate the genetic diversity and propose protective measures. Species identification is the most significant task in many fields of biological research and in conservation efforts. DNA barcoding, which constitutes the analysis of a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence, has been widely used for species identification. In this study, we collected 561 COI barcode sequences from 35 fish from the midstream of the Yangtze River. The intraspecific distances of all species were below 2% (with the exception of Acheilognathus macropterus and Hemibarbus maculatus). Nevertheless, all species could be unambiguously identified from the trees, barcoding gaps and taxonomic resolution ratio values. Furthermore, the COI barcode diversity was found to be low (≤0.5%), with the exception of H. maculatus (0.87%), A. macropterus (2.02%) and Saurogobio dabryi (0.82%). No or few shared haplotypes were detected between the upstream and downstream populations for ten species with overall nucleotide diversities greater than 0.00%, which indicated the likelihood of significant population genetic structuring. Our analyses indicated that DNA barcoding is an effective tool for the identification of cyprinidae fish in the midstream of the Yangtze River. It is vital that some protective measures be taken immediately because of the low COI barcode diversity.

  15. Dynamics of Rex3 in the genomes of endangered Iberian Leuciscinae (Teleostei, Cyprinidae and their natural hybrids

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    Carla Sofia A. Pereira

    2015-10-01

    Full Text Available Iberian Leuciscinae are greatly diverse comprising taxa of hybrid origin. With highly conservative karyotypes, Iberian Chondrostoma s.l. have recently demonstrated sub-chromosomal differentiation and rapid genome restructuring in natural hybrids, which was confirmed by ribosomal DNA (rDNA transposition and/or multiplication. To understand the role of repetitive DNAs in the differentiation of their genomes, a genetic and molecular cytogenetic survey was conducted in Achondrostoma oligolepis, Anaecypris hispanica, Iberochondrostoma lemmingii, I. lusitanicum, Pseudochondrostoma duriense, P. polylepis, Squalius pyrenaicus and hybrids between A. oligolepis x (P. duriense/P. polylepis, which represent 'alburnine', chondrostomine and Squalius lineages. The chromosomal distribution of Rex3 retroelement was found highly compartmentalized at centromeres and moderately at telomeres, co-localizing with 5S rDNA loci, and grossly correlating with heterochromatin and blocks of C0t-1 DNA. This accumulation was evident in at least 10 chromosome pairs, a pattern that seemed to be shared among the different species, likely predating their divergence. Nevertheless, species-specific clusters were detected in I. lusitanicum, P. duriense, P. polylepis and S. pyrenaicus demonstrating rapid and independent differentiation. Natural hybrids followed the same accumulation pattern and association with repetitive sequences but with increased number of Rex3 clusters and correlating with translocated 45S rDNA clusters. Rex3 sequence phylogeny didn't agree with its hosts' phylogeny but the observed distribution pattern is congruent with an evolutionary tendency to protect its activity, a robust regulatory system and/or events of horizontal transfer. This is the first report of retroelement physical mapping in Cyprinidae. It helped outlining conceivable ancestral homologies and recognizing retrotransposon activation in hybrids, being possibly associated with genome

  16. Geometric and morphometric analysis of fish scales to identity genera, species and populations case study: the Cyprinid family

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    Seyedeh Narjes Tabatabei

    2014-01-01

    Full Text Available Using fish scale to identity species and population is a rapid, safe and low cost method. Hence, this study was carried out to investigate the possibility of using geometric and morphometric methods in fish scales for rapid identification of species and populations and compare the efficiency of applying few and/or high number of landmark points. For this purpose, scales of one population of Luciobarbus capito, four populations of Alburnoides eichwaldii and two populations of Rutilus frisii kutum, all belonging to cyprinid family, were examined. On two-dimensional images of the scales 7 and 23 landmark points were digitized in two separate times using TpsDig2, respectively. Landmark data after generalized procrustes analysis were analyzed using Principal Component Analysis (PCA, Canonical Variate Analysis (CVA and Cluster Analysis. The results of both methods (using 7 and 23 landmark points showed significant differences of the shape of scales among the three species studied (P0.05. The results also showed that few number of landmarks could display the differences between scale shapes. According to the results of this study, it could be stated that the scale of each species had unique shape patterns which could be utilized as a species identification key.

  17. Histological damage and inflammatory response elicited by Monobothrium wageneri (Cestoda in the intestine of Tinca tinca (Cyprinidae

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    Sayyaf Dezfuli Bahram

    2011-12-01

    Full Text Available Abstract Background Among the European cyprinids, tench, Tinca tinca (L., and the pathological effects their cestodes may effect, have received very little or no attention. Most literature relating to Monobothrium wageneri Nybelin, 1922, a common intestinal cestode of tench, for example, has focused on aspects of its morphology rather than on aspects of the host-parasite interaction. Results Immunopathological and ultrastructural studies were conducted on the intestines of 28 tench, collected from Lake Piediluco, of which 16 specimens harboured tight clusters of numerous M. wageneri attached to the intestinal wall. The infection was associated with the degeneration of the mucosal layer and the formation of raised inflammatory swelling surrounding the worms. At the site of infection, the number of granulocytes in the intestine of T. tinca was significantly higher than the number determined 1 cm away from the site of infection or the number found in uninfected fish. Using transmission electron microscopy, mast cells and neutrophils were frequently observed in close proximity to, and inside, the intestinal capillaries; often these cells were in contact with the cestode tegument. At the host-parasite interface, no secretion from the parasite's tegument was observed. Intense degranulation of the mast cells was seen within the submucosa and lamina muscularis, most noticeably at sites close to the tegument of the scolex. In some instances, rodlet cells were encountered in the submucosa. In histological sections, hyperplasia of the mucous cells, notably those giving an alcian blue positive reaction, were evident in the intestinal tissues close to the swelling surrounding the worms. Enhanced mucus secretion was recorded in the intestines of infected tench. Conclusions The pathological changes and the inflammatory cellular response induced by the caryophyllidean monozoic tapeworm M. wageneri within the intestinal tract of an Italian population of wild

  18. Down-regulation of the cyprinid herpesvirus-3 annotated genes in cultured cells maintained at restrictive high temperature.

    Science.gov (United States)

    Ilouze, Maya; Dishon, Arnon; Kotler, Moshe

    2012-10-01

    Cyprinid herpesvirus-3 (CyHV-3) is a member of the Alloherpesviridae, in the order Herpesvirales. It causes a fatal disease in carp and koi fish. The disease is seasonal and is active when water temperatures ranges from 18 to 28 °C. Little is known about how and where the virus is preserved between the permissive seasons. The hallmark of the herpesviruses is their ability to become latent, persisting in the host in an apparently inactive state for varying periods of time. Hence, it could be expected that CyHV-3 enter a latent period. CyHV-3 has so far been shown to persist in fish maintained under restrictive temperatures, while shifting the fish to permissive conditions reactivates the virus. Previously, we demonstrated that cultured cells infected with CyHV-3 at 22 °C and subsequently transferred to a restrictive temperature of 30 °C preserve the virus for 30 days. The present report shows that cultured carp cells maintained and exposed to CyHV-3 at 30 °C are abortively infected; that is, autonomous viral DNA synthesis is hampered and the viral genome is not multiplied. Under these conditions, 91 of the 156 viral annotated ORFs were initially transcribed. These transcripts were down-regulated and gradually shut off over 18 days post-infection, while two viral transcripts encoded by ORFs 114 and 115 were preserved in the infected cells for 18 days p.i. These experiments, carried out in cultured cells, suggest that fish could be infected at a high non-permissive temperature and harbor the viral genome without producing viral particles. Copyright © 2012 Elsevier B.V. All rights reserved.

  19. Rational development of an attenuated recombinant cyprinid herpesvirus 3 vaccine using prokaryotic mutagenesis and in vivo bioluminescent imaging.

    Science.gov (United States)

    Boutier, Maxime; Ronsmans, Maygane; Ouyang, Ping; Fournier, Guillaume; Reschner, Anca; Rakus, Krzysztof; Wilkie, Gavin S; Farnir, Frédéric; Bayrou, Calixte; Lieffrig, François; Li, Hong; Desmecht, Daniel; Davison, Andrew J; Vanderplasschen, Alain

    2015-02-01

    Cyprinid herpesvirus 3 (CyHV 3) is causing severe economic losses worldwide in common and koi carp industries, and a safe and efficacious attenuated vaccine compatible with mass vaccination is needed. We produced single deleted recombinants using prokaryotic mutagenesis. When producing a recombinant lacking open reading frame 134 (ORF134), we unexpectedly obtained a clone with additional deletion of ORF56 and ORF57. This triple deleted recombinant replicated efficiently in vitro and expressed an in vivo safety/efficacy profile compatible with use as an attenuated vaccine. To determine the role of the double ORF56-57 deletion in the phenotype and to improve further the quality of the vaccine candidate, a series of deleted recombinants was produced and tested in vivo. These experiments led to the selection of a double deleted recombinant lacking ORF56 and ORF57 as a vaccine candidate. The safety and efficacy of this strain were studied using an in vivo bioluminescent imaging system (IVIS), qPCR, and histopathological examination, which demonstrated that it enters fish via skin infection similar to the wild type strain. However, compared to the parental wild type strain, the vaccine candidate replicated at lower levels and spread less efficiently to secondary sites of infection. Transmission experiments allowing water contamination with or without additional physical contact between fish demonstrated that the vaccine candidate has a reduced ability to spread from vaccinated fish to naïve sentinel cohabitants. Finally, IVIS analyses demonstrated that the vaccine candidate induces a protective mucosal immune response at the portal of entry. Thus, the present study is the first to report the rational development of a recombinant attenuated vaccine against CyHV 3 for mass vaccination of carp. We also demonstrated the relevance of the CyHV 3 carp model for studying alloherpesvirus transmission and mucosal immunity in teleost skin.

  20. Functional characterization of viral tumor necrosis factor receptors encoded by cyprinid herpesvirus 3 (CyHV3) genome.

    Science.gov (United States)

    Yi, Yang; Qi, Hemei; Yuan, Jimin; Wang, Rui; Weng, Shaoping; He, Jianguo; Dong, Chuanfu

    2015-08-01

    Cyprinid herpesvirus 3 (CyHV3) is a large double-stranded DNA virus of Alloherpesviridae family in the order Herpesvirales. It causes significant morbidity and mortality in common carp and its ornamental koi variety, and threatens the aquaculture industries worldwide. Mimicry of cytokines and cytokine receptors is a particular strategy for large DNA viruses in modulating the host immune response. Here, we report the identification and characterization of two novel viral homologues of tumor necrosis factor receptor (TNFR) encoded by CyHV3-ORF4 and -ORF12, respectively. CyHV3-ORF4 was identified as a homologue of HVEM and CyHV3-ORF12 as a homologue of TNFRSF1. Overexpression of ORF4 and ORF12 in zebrafish embryos results in embryonic lethality, morphological defects and increased apoptosis. Although we failed to identify any interaction between the two vTNFRs and their potential ligands in zebrafish TNF superfamily by yeast two-hybrid system, the expression of some genes in TNF superfamily or TNFR superfamily were mis-regulated in ORF4 or ORF12-overexpressing embryos, especially the death receptor zHDR and its cognate ligand DL1b. Further studies showed that the apoptosis induced by the both CyHV3 vTNFRs is mainly activated through the intrinsic apoptotic pathway and requires the crosstalk between the intrinsic and extrinsic apoptotic pathway. Additionally, using RT-qPCR and Western blot assays, the expression patterns of the both vTNFRs were also analyzed during CyHV3 productive infection. Collectively, this is the first functional study of two unique vTNFRs encoded by a herpesvirus infecting non-mammalian vertebrates, which may provide novel insights into viral immune regulation mechanism and the pathogenesis of CyHV3 infection. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. Early Jurassic diversification of pycnodontiform fishes (Actinopterygii, Neopterygii) after the end-Triassic extinction event: evidence from a new genus and species, Grimmenodon aureum.

    Science.gov (United States)

    Stumpf, Sebastian; Ansorge, Jörg; Pfaff, Cathrin; Kriwet, Jürgen

    2017-07-04

    A new genus and species of pycnodontiform fishes, Grimmenodon aureum , from marginal marine, marine-brackish lower Toarcian ( Harpoceras exaratum ammonite subzone) clay deposits of Grimmen in northeastern Germany is described. The single specimen represents a diagnostic left prearticular dentition characterized by unique tooth arrangement and ornamentation patterns. Grimmenodon aureum , gen. et sp. nov., is the second unambiguously identified pycnodontiform species from the Early Jurassic, in addition to Eomesodon liassicus from the early Lower Jurassic of western Europe. We also report an indeterminate pycnodontiform tooth crown from the upper Pliensbachian ( Pleuroceras apyrenum ammonite subzone) of the same site. The material expands the Early Jurassic range of pycnodontiforms significantly northwards and confirms their presence before and immediately following the onset of the Toarcian Oceanic Anoxic Event (T-OAE) in the marginal marine ecosystems south of the Fennoscandian Shield. Moreover, the new records indicate that the Early Jurassic diversity of pycnodontiform fishes was greater than previously assumed and probably equaled that of the Late Triassic. Therefore, it is hypothesized that the Triassic-Jurassic mass extinction event did not affect pycnodontiform fishes significantly. Micro-computed tomography was used to study the internal anatomy of the prearticular of Grimmenodon aureum , gen. et sp. nov. Our results show that no replacement teeth were formed within the tooth-bearing bone but rather were added posteriorly to functional teeth. http://zoobank.org/urn:lsid:zoobank.org:pub:A56BDE9C-40C4-4CFA-9C2E-F5FA35A66F2 Citation for this article: Stumpf, S., J. Ansorge, C. Pfaff, and J. Kriwet. 2017. Early Jurassic diversification of pycnodontiform fishes (Actinopterygii, Neopterygii) after the end-Triassic extinction event: Evidence from a new genus and species, Grimmenodon aureum . Journal of Vertebrate Paleontology. DOI: 10.1080/02724634.2017.1344679.

  2. Detection of cyprinid herpesvirus 2 in peripheral blood cells of silver crucian carp, Carassius auratus gibelio (Bloch), suggests its potential in viral diagnosis.

    Science.gov (United States)

    Wang, H; Xu, Lj; Lu, Lq

    2016-02-01

    Epidemics caused by cyprinid herpesvirus 2 (CyHV-2) in domestic cyprinid species have been reported in both European and Asian countries. Although the mechanisms remain unknown, acute CyHV-2 infections generally result in high mortality, and the surviving carps become chronic carriers displaying no external clinical signs. In this study, in situ hybridization analysis showed that CyHV-2 tended to infect peripheral blood cells during either acute or chronic infections in silver crucian carp, Carassius auratus gibelio (Bloch). Laboratory challenge experiments coupled with real-time PCR quantification assays further indicated that steady-state levels of the viral genomic copy number in fish serum exhibited a typical 'one-step' growth curve post-viral challenge. Transcriptional expression of open reading frames (ORF) 121, which was selected due to its highest transcriptional levels in almost all tested tissues, was monitored to represent the replication kinetics of CyHV-2 in peripheral blood cells. Similar kinetic curve of active viral gene transcription in blood cells was obtained as that of serum viral load, indicating that CyHV-2 replicated in peripheral blood cells as well as in other well-characterized tissues. This study should pave the way for designing non-invasive and cost-effective serum diagnostic methods for quick detection of CyHV-2 infection. © 2015 John Wiley & Sons Ltd.

  3. HEMATOLOGICAL BLOOD PARAMETERS OF YOUNG-OF-THE-YEAR CARPS (CYPRINIDAE REARED USING FISH RANCHING TECHNOLOGY IN THE SOUTHERN UKRAINE

    Directory of Open Access Journals (Sweden)

    Yu. Volichenko

    2015-12-01

    the indication of the quality and general state of fish seeds. The changes in certain parameters in young-of-the-year cyprinids observed in the process of their growing suggest certain changes in their physiological conditions and may be considered as a specific signal for developing appropriate preventive actions and optimizing fish rearing process.

  4. Dieta de la carpa Notropis moralesi (Pisces: Cyprinidae en el río Amacuzac, Morelos, México

    Directory of Open Access Journals (Sweden)

    Patricia Trujillo-Jiménez

    2009-06-01

    Full Text Available Notropis moralesi, conocido comúnmente como "carpa tepelneme", es un pez endémico del río Papaloapan. Se analizó su dieta y hábitos alimentarios. Se tomaron muestras mensuales en el río Amacuzac, Morelos, México. La identificación del contenido estomacal (294 ejemplares se llevó hasta el taxon más específico posible. Para la cuantificación se utilizaron los métodos numérico (No y frecuencia de aparición (F.O.. Se utilizó el índice de amplitud de nicho trófico de Levin y el índice de traslape de MacArthur y Levin. N. moralesi presentó la ingestión de once componentes alimenticios, de los cuales diez son de origen animal y uno vegetal. Los quironómidos y efemerópteros fueron los más consumidos independientemente del sexo, talla y época del año. El análisis por épocas del año, reportó diez componentes en el estiaje y ocho en las lluvias. La dieta de los inmaduros estuvo constituida por diez componentes, la de las hembras por ocho y el de los machos por cinco. La prueba de Ji-cuadrada reveló que no existen diferencias significativas entre las dietas por estadio de desarrollo, sexos y épocas del año. El traslape de los nichos tróficos, reveló valores superiores a 0.60 en todas las combinaciones por sexos y épocas del año. N. moralesi es una especie carnívora con tendencias insectívoras y presenta hábitos alimentarios de tipo bentófago.Diet of the chub, Notropis moralesi (Pisces: Cyprinidae in the Amacuzac River, Morelos, Mexico. Notropis moralesi, locally known as "carpa tepelneme", is a small endemic fish of the Papaloapan River. We analyzed its diet and feeding habits. Specimens were captured monthly from Amacuzac, River, Morelos, Mexico. The gut content of 294 specimens was identified to the most specific taxonomic category possible. We quantified gut content by the numerical and frequency of occurrence methods and used the Levin’s amplitude of trophic niche indices and MacArthur and Levin`s indices for

  5. A new species of the genus Achondrostoma Robalo, Almada, Levy & Doadrio, 2007 (Actynopterigii, Cyprinidae from western Spain

    Directory of Open Access Journals (Sweden)

    Doadrio, I.

    2007-12-01

    Full Text Available A new freshwater fish species, Achondrostoma salmantinum, formerly considered as one population of Iberochondrostoma lemmingii, is described on the basis of genetic and morphological characters. The new species inhabits the rivers Huebra, Águeda and Uces and tributaries in the Duero River basin in Salamanca province, western Spain. The species is distinguished from Iberochondrostoma lemmingii and from other species of the genus Achondrostoma according to morphometric, meristic and genetic characters such as a narrow caudal peduncle, long postorbital and head lengths, small number of scales (9-10/44-53/4-6, small number of gill rakers (15-22, usually 5-5 pharyngeal teeth, four diagnostic isozyme loci (IDHP-2*, IDHP-3*, MDH-B* and PEP* and one unique allele (MDH-A*95. The distribution of I. lemmingii is currently known to be restricted to the Tajo, Guadiana, Odiel and Guadalquivir basins. This new cyprinid is considered Endangered (EN according to IUCN Red List Categories.

    Se describe una nueva especie de pez de agua dulce, Achondrostoma salmantinum, anteriormente considerada como una población de Iberochondrostoma lemmingii, a partir de caracteres genéticos y morfológicos. La nueva especie vive en la cuenca del río Duero en los ríos Uces, Huebra y Águeda y sus afluentes en la provincia de Salamanca en el oeste de España. De acuerdo con los caracteres morfométricos y genéticos la especie se distingue de Iberochondrostoma lemmingii y de otras especies del género Achondrostoma por un pedúnculo caudal estrecho, larga longitud de la cabeza y de la distancia postorbital, pequeño número de escamas (9/10/44-53/4-6, pequeño número de branquispinas (15-22, frecuentemente 5-5 dientes faríngeos, cuatro loci isozimáticos diagnósticos (IDHP-2*, IDHP-3*, MDH-B* y PEP* y un único alelo (MDH-A*95. La distribución de I. lemmingii se

  6. A new monozoic tapeworm, Lobulovarium longiovatum n. g., n. sp. (Cestoda: Caryophyllidea), from barbs Puntius spp. (Teleostei: Cyprinidae) in the Indomalayan region.

    Science.gov (United States)

    Oros, Mikuláš; Ash, Anirban; Brabec, Jan; Kar, Pradip Kumar; Scholz, Tomáš

    2012-09-01

    A new caryophyllidean cestode is described from barbs Puntius spp. (Cypriniformes: Cyprinidae), with P. sophore (Hamilton) as its type-host, in the Ganges and Brahmaputra river basins in India and Bangladesh, and a new genus, Lobulovarium n. g., is proposed to accommodate it. The genus belongs to the Lytocestidae because its vitelline follicles are situated in the cortex. It is typified by: (i) a peculiar ovary, which is roughly H-shaped, but with asymmetrical, irregular lobes on its ventral and dorsal sides; (ii) an extensive vitellarium formed by numerous vitelline follicles scattered throughout the cortex; (iii) a long, conical postovarian part of the body with numerous vitelline follicles; (iv) a broadly digitate scolex with a slightly protrusible central cone; (v) a single gonopore (male and female genital ducts open via a single pore and a common genital atrium is absent); and (vi) a small number of testes (90 μm in L. longiovatum), which are spherical (length/width ratio 1:1 versus 2.5-3:1 in the new species), and the presence of vitelline follicles alongside the ovarian lobes (almost completely absent in L. longiovatum).

  7. Effects of carbon nanomaterials fullerene C60 and fullerol C60(OH)18–22 on gills of fish Cyprinus carpio (Cyprinidae) exposed to ultraviolet radiation

    International Nuclear Information System (INIS)

    Socoowski Britto, Roberta; Longaray Garcia, Márcia; Martins da Rocha, Alessandra; Artigas Flores, Juliana; Pinheiro, Maurício V. Brant; Monserrat, José María; Ribas Ferreira, Josencler L.

    2012-01-01

    In consequence of their growing use and demand, the inevitable environmental presence of nanomaterials (NMs) has raised concerns about their potential deleterious effects to aquatic environments. The carbon NM fullerene (C 60 ), which forms colloidal aggregates in water, and its water-soluble derivative fullerol (C 60 (OH) 18–22 ), which possesses antioxidant properties, are known to be photo-excited by ultraviolet (UV) or visible light. To investigate their potential hazards to aquatic organisms upon exposure to UV sunlight, this study analyzed (a) the in vitro behavior of fullerene and fullerol against peroxyl radicals (ROO·) under UV-A radiation and (b) the effects of these photo-excited NMs on oxidative stress parameters in functional gills extracted from the fish Cyprinus carpio (Cyprinidae). The variables measured were the total antioxidant capacity, lipid peroxidation (TBARS), the activities of the antioxidant enzymes glutathione reductase (GR) and glutamate cysteine ligase (GCL), and the levels of the non-enzymatic antioxidant glutathione (GSH). The obtained results revealed the following: (1) both NMs behaved in vitro as antioxidants against ROO· in the dark and as pro-oxidants in presence of UV-A, the latter effect being reversed by the addition of sodium azide, which is a singlet oxygen ( 1 O 2 ) quencher; (2) fullerene induced toxicity with or without UV-A incidence, with a significant (p 1 O 2 generation; and (3) fullerol also decreased GCL activity and GSH formation (p 1 O 2 formation.

  8. Effects of different inorganic arsenic species in Cyprinus carpio (Cyprinidae) tissues after short-time exposure: Bioaccumulation, biotransformation and biological responses

    International Nuclear Information System (INIS)

    Ventura-Lima, Juliane; Fattorini, Daniele; Regoli, Francesco; Monserrat, Jose M.

    2009-01-01

    Differences in the toxicological and metabolic pathway of inorganic arsenic compounds are largely unknown for aquatic species. In the present study the effects of short-time and acute exposure to As III and As V were investigated in gills and liver of the common carp, Cyprinus carpio (Cyprinidae), measuring accumulation and chemical speciation of arsenic, and the activity of glutathione-S-transferase omega (GST Ω), the rate limiting enzyme in biotransformation of inorganic arsenic. Oxidative biomarkers included antioxidant defenses (total glutathione-S-transferases, glutathione reductase, glutathione, and glucose-6-phosphate dehydrogenase), total scavenging capacity toward peroxyl radicals, reactive oxygen species (ROS) measurement and lipid peroxidation products. A marked accumulation of arsenic was observed only in gills of carps exposed to 1000 ppb As V . Also in gills, antioxidant responses were mostly modulated through a significant induction of glucose-6-phosphate dehydrogenase activity which probably contributed to reduce ROS formation; however this increase was not sufficient to prevent lipid peroxidation. No changes in metal content were measured in liver of exposed carps, characterized by lower activity of GST Ω compared to gills. On the other hand, glutathione metabolism was more sensitive in liver tissue, where a significant inhibition of glutathione reductase was concomitant with increased levels of glutathione and higher total antioxidant capacity toward peroxyl radicals, thus preventing lipid peroxidation and ROS production. The overall results of this study indicated that exposure of C. carpio to As III and As V can induce different responses in gills and liver of this aquatic organism. - Common carp (Cyprinus carpio) presented marked differences between gills and liver after arsenic exposure in terms of antioxidant responses and also in biotransformation.

  9. Effects of different inorganic arsenic species in Cyprinus carpio (Cyprinidae) tissues after short-time exposure: Bioaccumulation, biotransformation and biological responses

    Energy Technology Data Exchange (ETDEWEB)

    Ventura-Lima, Juliane [Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS (Brazil); Programa de Pos-Graduacao em Ciencias Fisiologicas - Fisiologia Animal Comparada (FURG), Rio Grande, RS (Brazil); Fattorini, Daniele; Regoli, Francesco [Istituto di Biologia e Genetica, Universita Politecnica delle Marche, 60100, Ancona (Italy); Monserrat, Jose M., E-mail: josemmonserrat@pesquisador.cnpq.b [Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS (Brazil); Programa de Pos-Graduacao em Ciencias Fisiologicas - Fisiologia Animal Comparada (FURG), Rio Grande, RS (Brazil)

    2009-12-15

    Differences in the toxicological and metabolic pathway of inorganic arsenic compounds are largely unknown for aquatic species. In the present study the effects of short-time and acute exposure to As{sup III} and As{sup V} were investigated in gills and liver of the common carp, Cyprinus carpio (Cyprinidae), measuring accumulation and chemical speciation of arsenic, and the activity of glutathione-S-transferase omega (GST OMEGA), the rate limiting enzyme in biotransformation of inorganic arsenic. Oxidative biomarkers included antioxidant defenses (total glutathione-S-transferases, glutathione reductase, glutathione, and glucose-6-phosphate dehydrogenase), total scavenging capacity toward peroxyl radicals, reactive oxygen species (ROS) measurement and lipid peroxidation products. A marked accumulation of arsenic was observed only in gills of carps exposed to 1000 ppb As{sup V}. Also in gills, antioxidant responses were mostly modulated through a significant induction of glucose-6-phosphate dehydrogenase activity which probably contributed to reduce ROS formation; however this increase was not sufficient to prevent lipid peroxidation. No changes in metal content were measured in liver of exposed carps, characterized by lower activity of GST OMEGA compared to gills. On the other hand, glutathione metabolism was more sensitive in liver tissue, where a significant inhibition of glutathione reductase was concomitant with increased levels of glutathione and higher total antioxidant capacity toward peroxyl radicals, thus preventing lipid peroxidation and ROS production. The overall results of this study indicated that exposure of C. carpio to As{sup III} and As{sup V} can induce different responses in gills and liver of this aquatic organism. - Common carp (Cyprinus carpio) presented marked differences between gills and liver after arsenic exposure in terms of antioxidant responses and also in biotransformation.

  10. Hepatocyte nuclear structure and subcellular distribution of copper in zebrafish Brachydanio rerio and roach Rutilus rutilus (Teleostei, Cyprinidae) exposed to copper sulphate

    Energy Technology Data Exchange (ETDEWEB)

    Paris-Palacios, Severine [Universite de Reims Champagne-Ardenne (URCA), UFR Sciences Exactes et Naturelles, Laboratoire d' Eco-Toxicologie, Institut International de Recherche sur les Ions Metalliques, B.P. 1039-51687 Reims cedex 2 (France)]. E-mail: severine.paris@univ-reims.fr; Biagianti-Risbourg, Sylvie [Universite de Reims Champagne-Ardenne (URCA), UFR Sciences Exactes et Naturelles, Laboratoire d' Eco-Toxicologie, Institut International de Recherche sur les Ions Metalliques, B.P. 1039-51687 Reims cedex 2 (France)]. E-mail: sylvie.biagianti@univ-reims.fr

    2006-05-10

    Copper is a trace element essential to life, but also a heavy metal with toxic effect clearly demonstrated. Cu induced perturbations in fish liver are well documented but the variability of the reported results is large. In this study two cyprinids, zebrafish and roach, were exposed to copper. Reported histocytological changes are either adaptative or degenerative depending on fish species, concentration of metal, and duration of exposure. Hepatic subcellular distribution of copper was determined by X-ray microanalysis in control and Cu-exposed roach and zebrafish. Sublethal copper sulphate contamination induced the development of a particular nucleolar alteration forming a network or honeycomb like structure in liver. This perturbation is observable in almost all the hepatocytes of zebrafish and roach exposed to copper for a minimum of 4 days of exposure. It seemed to concern more precisely the pars fibrosa. X-ray microanalysis showed that the appearance of network nucleolus was in relation to a Cu accumulation. Cu deposit was well located in the network as pars granulosa and nucloplasm showed very lower metal concentrations. The origin and consequence of network structure in nucleolus was discussed.

  11. Hepatocyte nuclear structure and subcellular distribution of copper in zebrafish Brachydanio rerio and roach Rutilus rutilus (Teleostei, Cyprinidae) exposed to copper sulphate

    International Nuclear Information System (INIS)

    Paris-Palacios, Severine; Biagianti-Risbourg, Sylvie

    2006-01-01

    Copper is a trace element essential to life, but also a heavy metal with toxic effect clearly demonstrated. Cu induced perturbations in fish liver are well documented but the variability of the reported results is large. In this study two cyprinids, zebrafish and roach, were exposed to copper. Reported histocytological changes are either adaptative or degenerative depending on fish species, concentration of metal, and duration of exposure. Hepatic subcellular distribution of copper was determined by X-ray microanalysis in control and Cu-exposed roach and zebrafish. Sublethal copper sulphate contamination induced the development of a particular nucleolar alteration forming a network or honeycomb like structure in liver. This perturbation is observable in almost all the hepatocytes of zebrafish and roach exposed to copper for a minimum of 4 days of exposure. It seemed to concern more precisely the pars fibrosa. X-ray microanalysis showed that the appearance of network nucleolus was in relation to a Cu accumulation. Cu deposit was well located in the network as pars granulosa and nucloplasm showed very lower metal concentrations. The origin and consequence of network structure in nucleolus was discussed

  12. Effects of water quality and trophic status on helminth infections in the cyprinid fish, Schizothorax niger Heckel, 1838 from three lakes in the Kashmir Himalayas.

    Science.gov (United States)

    Zargar, U R; Yousuf, A R; Chishti, M Z; Ahmed, F; Bashir, H; Ahmed, F

    2012-03-01

    Water quality greatly influences the population density of aquatic biota, including parasites. In order to evaluate the relationship between fish parasites and water quality in Kashmir Himalayas, we assessed helminth parasite densities in Schizothorax niger Heckel, 1838 (an endemic cyprinid fish of Kashmir) from three lakes, namely Anchar, Manasbal and Dal, which reflected the varied stages of eutrophication. The overall prevalence of helminth infections was higher in the hypertrophic Anchar Lake (prevalence = 18.6%) compared to Manasbal Lake, which was the least eutrophied (prevalence = 6.4%). Furthermore, mean prevalence of monoxenous and heteroxenous parasites was higher in lakes containing higher levels of water degradation (Anchar and Dal). The mean number of helminth species per fish host was the highest in the hypertrophic lake (1.3 ± 0.3) in comparison to the least eutrophic lake (0.2 ± 1.5). Variability of calculated infection indices (prevalence, mean intensity and mean abundance) revealed that helminth parasite composition in the fish was affected by the lakes' environmental stress (degraded water quality). Therefore, data on the density of helminth parasites in fish can provide supplementary information on the pollution status of a water body.

  13. Socializing makes thick-skinned individuals: on the density of epidermal alarm substance cells in cyprinid fish, the crucian carp (Carassius carassius).

    Science.gov (United States)

    Stabell, Ole B; Vegusdal, Anne

    2010-09-01

    In cyprinid fish, density of epidermal club cells (i.e. alarm substance cells) has been found to vary between lakes with different predator fauna. Because predators can be labelled with chemical cues from prey, we questioned if club cell density could be controlled indirectly by predators releasing prey cues. In particular, we suspected a possible feedback mechanism between chemical alarm signals and their cellular source. We raised crucian carp singly and in groups of four. For both rearing types, fish were exposed to skin extracts of either conspecifics or brown trout (without club cells), and provided either low or high food rations. Independent of rearing type, condition factor and club cell density increased with food ration size, but no change was found in club cell density following exposure to conspecific alarm signals. However, the density of club cells was found significantly higher for fish raised in groups than for fish raised alone. We conclude that an increased condition factor results in more club cells, but crucian carp may also possess an awareness of conspecific presence, given by higher club cell densities when raised in groups. This increase in club cell density may be induced by unknown chemical factors released by conspecifics.

  14. Invasive cyprinid fish in Europe originate from the single introduction of an admixed source population followed by a complex pattern of spread.

    Directory of Open Access Journals (Sweden)

    Andrea Simon

    Full Text Available The Asian cyprinid fish, the topmouth gudgeon (Pseudorasbora parva, was introduced into Europe in the 1960s. A highly invasive freshwater fish, it is currently found in at least 32 countries outside its native range. Here we analyse a 700 base pair fragment of the mitochondrial cytochrome b gene to examine different models of colonisation and spread within the invasive range, and to investigate the factors that may have contributed to their invasion success. Haplotype and nucleotide diversity of the introduced populations from continental Europe was higher than that of the native populations, although two recently introduced populations from the British Isles showed low levels of variability. Based on coalescent theory, all introduced and some native populations showed a relative excess of nucleotide diversity compared to haplotype diversity. This suggests that these populations are not in mutation-drift equilibrium, but rather that the relative inflated level of nucleotide diversity is consistent with recent admixture. This study elucidates the colonisation patterns of P. parva in Europe and provides an evolutionary framework of their invasion. It supports the hypothesis that their European colonisation was initiated by their introduction to a single location or small geographic area with subsequent complex pattern of spread including both long distance and stepping-stone dispersal. Furthermore, it was preceded by, or associated with, the admixture of genetically diverse source populations that may have augmented its invasive-potential.

  15. Comparative analysis of differential gene expression in kidney tissues of moribund and surviving crucian carp (Carassius auratus gibelio) in response to cyprinid herpesvirus 2 infection.

    Science.gov (United States)

    Xu, Lijuan; Podok, Patarida; Xie, Jun; Lu, Liqun

    2014-08-01

    Cyprinid herpesvirus 2 (CyHV-2) has recently been associated with high mortality of cultured crucian carp (Carassius auratus gibelio) in eastern China. In this study, we established a real-time PCR method to confirm viral infection of crucian carp and to quantify CyHV-2 particles obtained by sucrose gradient centrifugation from diseased fish. Virus-free crucian carp were artificially infected with CyHV-2 using an injection method, which resulted in a dose-dependent death rate. In situ hybridization analysis indicated that there was extensive viral replication and lysis in the kidneys of moribund fish, in contrast to very limited replication in surviving fish. To probe the host immune response to viral infection at the level of gene expression, we identified virus-responsive genes using suppression subtractive hybridization (SSH) in head kidney tissues, the principal immune organ of fish, from moribund and surviving crucian carps after viral challenge. From the moribund SSH library, 363 expressed sequence tags (ESTs) were clustered to 234 unigenes (including 15 singletons and 45 contigs). From the survivor SSH library, 599 ESTs was clustered to 549 unigenes (including 107 singletons and 105 contigs). We further analyzed the transcriptional levels of all immune-related genes by quantitative real-time RT-PCR, which confirmed the upregulation of 90.48 % of these genes. The significantly upregulated immune-related genes identified in this study can serve as candidate marker genes for acute CyHV-2 infection.

  16. Validation of a serum neutralization test for detection of antibodies specific to cyprinid herpesvirus 3 in infected common and koi carp (Cyprinus carpio).

    Science.gov (United States)

    Cabon, J; Louboutin, L; Castric, J; Bergmann, S; Bovo, G; Matras, M; Haenen, O; Olesen, N J; Morin, T

    2017-05-01

    Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of a serious infective, notifiable disease affecting common carp and varieties. In survivors, infection is generally characterized by a subclinical latency phase with restricted viral replication. The CyHV-3 genome is difficult to detect in such carrier fish that represent a potential source of dissemination if viral reactivation occurs. In this study, the analytical and diagnostic performance of an alternative serum neutralization (SN) method based on the detection of CyHV-3-specific antibodies was assessed using 151 serum or plasma samples from healthy and naturally or experimentally CyHV-3-infected carp. French CyHV-3 isolate 07/108b was neutralized efficiently by sera from carp infected with European, American and Taiwanese CyHV-3 isolates, but no neutralization was observed using sera specific to other aquatic herpesviruses. Diagnostic sensitivity, diagnostic specificity and repeatability of 95.9%, 99.0% and 99.3%, respectively, were obtained, as well as a compliance rate of 89.9% in reproducibility testing. Neutralizing antibodies were steadily detected in infected carp subjected to restrictive or permissive temperature variations over more than 25 months post-infection. The results suggest that this non-lethal diagnostic test could be used in the future to improve the epidemiological surveillance and control of CyHV-3 disease. © 2016 John Wiley & Sons Ltd.

  17. Antibody screening identifies 78 putative host proteins involved in Cyprinid herpesvirus 3 infection or propagation in common carp, Cyprinus carpio L.

    Science.gov (United States)

    Gotesman, M; Soliman, H; El-Matbouli, M

    2014-01-01

    Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of a serious and notifiable disease afflicting common and koi carp, Cyprinus carpio L., termed koi herpesvirus disease (KHVD). Significant progress has been achieved in the last 15 years, since the initial reports surfaced from Germany, USA and Israel of the CyHV-3 virus, in terms of pathology and detection. However, relatively few studies have been carried out in understanding viral replication and propagation. Antibody-based affinity has been used for detection of CyHV-3 in enzyme-linked immunosorbent assay and PCR-based techniques, and immunohistological assays have been used to describe a CyHV-3 membrane protein, termed ORF81. In this study, monoclonal antibodies linked to N-hydroxysuccinimide (NHS)-activated spin columns were used to purify CyHV-3 and host proteins from tissue samples originating in either CyHV-3 symptomatic or asymptomatic fish. The samples were next analysed either by polyacrylamide gel electrophoresis (PAGE) and subsequently by electrospray ionization coupled to mass spectrometry (ESI-MS) or by ESI-MS analysis directly after purification. A total of 78 host proteins and five CyHV-3 proteins were identified in the two analyses. These data can be used to develop novel control methods for CyHV-3, based on pathways or proteins identified in this study. PMID:23347276

  18. Ascent ability of brown trout, Salmo trutta, and two Iberian cyprinids − Iberian barbel, Luciobarbus bocagei, and northern straight-mouth nase, Pseudochondrostoma duriense − in a vertical slot fishway

    Science.gov (United States)

    Sanz-Ronda, Fco. Javier; Bravo-Cordoba, F.J.; Fuentes-Perez, J.F.; Castro-Santos, Theodore R.

    2016-01-01

    Passage performance of brown trout (Salmo trutta), Iberian barbel (Luciobarbus bocagei), and northern straight-mouth nase (Pseudochondrostoma duriense) was investigated in a vertical slot fishway in the Porma River (Duero River basin, Spain) using PIT telemetry. We analysed the effects of different fishway discharges on motivation and passage success. Both cyprinid species ascended the fishway easily, performing better than the trout despite their theoretically weaker swimming performance. Fishway discharge affected fish motivation although it did not clearly influence passage success. Observed results can guide design and operation criteria of vertical slot fishways for native Iberian fish.

  19. Morphological and genetic evolution in eastern populations of the Macrhybopsis aestivalis complex (Cypriniformes: Cyprinidae), with the descriptions of four new species.

    Science.gov (United States)

    Gilbert, Carter R; Mayden, Richard L; Powers, Steven L

    2017-03-30

    For many years the North American cyprinid fish Macrhybopsis aestivalis (common name: Speckled Chub) was regarded as a single widespread and morphologically variable species, occurring in rivers throughout much of the Mississippi Valley and geographically adjacent eastern Gulf slope drainages, west to the Rio Grande basin in Texas, New Mexico, and Mexico. Eisenhour (1997) completed a morphological study of western populations of the Speckled Chub, the results of which appeared thereafter in published form (Eisenhour 1999, 2004). He demonstrated the existence of five valid species west of the Mississippi River (aestivalis, marconis, australis, tetranema, hyostoma), of which the name aestivalis was shown to be restricted to the population occurring in the Rio Grande and the geographically adjacent Rio San Fernando system, in northeastern Mexico. Eisenhour (2004) considered populations throughout the middle Mississippi Valley and its major tributaries to be a single morphologically variable species (hyostoma), and he also indicated that populations of Macrhybopsis from eastern Gulf slope drainages may represent a complex of species. Genetic confirmation of Eisenhour's conclusions regarding western species appeared in the publication by Underwood et al. (2003), who also showed that western populations of M. hyostoma, as presently recognized, are genetically much more complex than previously considered.     Meanwhile, the present authors were involved in a companion study of eastern populations of Macrhybopsis, for which a genetic summary of the eastern Gulf coast species was published by Mayden & Powers (2004). Based on their findings, four species were recognized from southeastern drainages (identified as species A-D), although no formal taxonomic descriptions were included. Their genetic data, in combination with meristic, morphometric and other morphological data presented herein, form the basis for a revised classification of eastern Macrhybopsis populations

  20. Isolation and characterisation of mRNA encoding the salmon- and chicken-II type gonadotrophin-releasing hormones in the teleost fish Rutilus rutilus (Cyprinidae).

    Science.gov (United States)

    Penlington, M C; Williams, M A; Sumpter, J P; Rand-Weaver, M; Hoole, D; Arme, C

    1997-12-01

    The complementary DNAs (cDNA) encoding the [Trp7,Leu8]-gonadotrophin-releasing hormone (salmon-type GnRH; sGnRH:GeneBank accession no. u60667) and the [His5,Trp7,Tyr8]-GnRH (chicken-II-type GnRH; cGnRH-II: GeneBank accession no. u60668) precursor in the roach (Rutilus rutilus) were isolated and sequenced following reverse transcription and rapid amplification of cDNA ends (RACE). The sGnRH and cGnRH-II precursor cDNAs consisted of 439 and 628 bp, and included open reading frames of 282 and 255 bp respectively. The structures of the encoded peptides were the same as GnRHs previously identified in other vertebrates. The sGnRH and cGnRH-II precursor cDNAs, including the non-coding regions, had 88.6 and 79.9% identity respectively, to those identified in goldfish (Carassius auratus). However, significant similarity was not observed between the non-coding regions of the GnRH cDNAs of Cyprinidae and other fish. The presumed third exon, encoding partial sGnRH associated peptide (GAP) of roach, demonstrated significant nucleotide and amino acid similarity with the appropriate regions in the goldfish, but not with other species, and this may indicate functional differences of GAP between different families of fish. cGnRH-II precursor cDNAs from roach had relatively high nucleotide similarity across this GnRH variant. Cladistic analysis classified the sGnRH and cGnRH-II precursor cDNAs into three and two groups respectively. However, the divergence between nucleotide sequences within the sGnRH variant was greater than those encoding the cGnRH-II precursors. Consistent with the consensus developed from previous studies, Northern blot analysis demonstrated that expression of sGnRH and cGnRH-II was restricted to the olfactory bulbs and midbrain of roach respectively. This work forms the basis for further study on the mechanisms by which the tapeworm, Ligula intestinalis, interacts with the pituitary-gonadal axis of its fish host.

  1. When Anthropogenic River Disturbance Decreases Hybridisation between Non-Native and Endemic Cyprinids and Drives an Ecomorphological Displacement towards Juvenile State in Both Species.

    Directory of Open Access Journals (Sweden)

    Emmanuel Corse

    Full Text Available Understanding the impact of non-native species on native species is a major challenge in molecular ecology, particularly for genetically compatible fish species. Invasions are generally difficult to study because their effects may be confused with those of environmental or human disturbances. Colonized ecosystems are differently impacted by human activities, resulting in diverse responses and interactions between native and non-native species. We studied the dynamics between two Cyprinids species (invasive Chondrostoma nasus and endemic Parachondrostoma toxostoma and their hybrids in 16 populations (from allopatric to sympatric situations and from little to highly fragmented areas corresponding to 2,256 specimens. Each specimen was assigned to a particular species or to a hybrid pool using molecular identification (cytochrome b and 41 microsatellites. We carried out an ecomorphological analysis based on size, age, body shape, and diet (gut vacuity and molecular fecal contents. Our results contradicted our initial assumptions on the pattern of invasion and the rate of introgression. There was no sign of underperformance for the endemic species in areas where hybridisation occurred. In the unfragmented zone, the introduced species was found mostly downstream, with body shapes similar to those in allopatric populations while both species were found to be more insectivorous than the reference populations. However, high level of hybridisation was detected, suggesting interactions between the two species during spawning and/or the existence of hybrid swarm. In the disturbed zone, introgression was less frequent and slender body shape was associated with diatomivorous behaviour, smaller size (juvenile characteristics and greater gut vacuity. Results suggested that habitat degradation induced similar ecomorphological trait changes in the two species and their hybrids (i.e. a transition towards a pedomorphic state where the invasive species is more

  2. Whole-genome sequence of a novel Chinese cyprinid herpesvirus 3 isolate reveals the existence of a distinct European genotype in East Asia.

    Science.gov (United States)

    Li, Wei; Lee, Xuezhu; Weng, Shaoping; He, Jianguo; Dong, Chuanfu

    2015-02-25

    Cyprinid herpesvirus 3 (CyHV3), also known as koi herpesvirus (KHV), can be subdivided primarily into European and Asian genotypes, which are represented by CyHV3-U or CyHV3-I and CyHV3-J, respectively. In this study, the whole genome sequence of a novel Chinese CyHV3 isolate (GZ11) was determined and annotated. CyHV3-GZ11 genome was found to contain 295,119 nucleotides with 52.9% G/C content, which is highly similar to those of published CyHV3-U, CyHV3-I, and CyHV3-J strains. With reference to CyHV3-U, CyHV3-I, and CyHV3-J, CyHV3-GZ11 was also classified into 164 open reading frames (ORF), which include eight repeated ORFs. On the basis of the 12 alloherpeviruses core genes, results from phylogenetic analysis showed that CyHV3-GZ11 had closer evolutionary relationships with CyHV3-U and CyHV3-I than with CyHV3/KHV-J, which were also supported by genome wide-based single nucleotide substitution analysis and the use of a series of developed molecular markers. This study was the first to reveal the presence of a distinct European CyHV3 genotype in East and Southeast Asia at a whole genome level, which will evoke new insights on exploring the origin, evolution, and epidemiology of the virus. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Establishment of a novel and highly permissive cell line for the efficient replication of cyprinid herpesvirus 2 (CyHV-2).

    Science.gov (United States)

    Ma, Jie; Jiang, Nan; LaPatra, Scott E; Jin, Ling; Xu, Jin; Fan, Yuding; Zhou, Yong; Zeng, Lingbing

    2015-06-12

    Haematopoietic necrosis of gibel carp (Carassius auratus gibelio) is caused by cyprinid herpesvirus 2 (CyHV-2) and has caused huge economic losses in aquaculture worldwide. Currently the isolation and propagation of CyHV-2 in vitro is very difficult due to the lack of permissive cell lines. Studies on the pathogenesis of CyHV-2 have been hampered because the virus has not been extensively characterized. In this study, a novel cell line from the brain of gibel carp, denoted GiCB, has been established and characterized. Sustainable propagation of CyHV-2 in the GiCB cell line has been confirmed by virus infection and titration, PCR, transmission electron microscopy, immunofluorescence assay and fluorescence in situ hybridization. The GiCB cells showed typical cytopathic effect by day 6 post-infection with CyHV-2 including cell shrinkage, rounding, and cell fusion with cytoplasmic vacuolization. The virus titer reached 10(7.5 ± 0.37)TCID₅₀/ml and has been successfully passaged over 50 times in the GiCB cell line. Electron microscopy analysis revealed the complete replication of CyHV-2 in GiCB cells. CyHV-2-infected GiCB cells reacted strongly with polyclonal antibodies against CyHV-2 and CyHV-2 RNA in cells hybridized specifically with the virus RNA probes. Additionally, an experimental infection demonstrated that CyHV-2 produced in GiCB cells caused 100% mortality in gibel carp. All the results provide solid evidence that the GiCB cell line is highly permissive for the isolation and propagation of CyHV-2. This is a significant advancement that will promote additional research on CyHV-2 infection in fish in the future. Copyright © 2015 Elsevier B.V. All rights reserved.

  4. Cyprinid herpesvirus-3 (CyHV-3) disturbs osmotic balance in carp (Cyprinus carpio L.)--A potential cause of mortality.

    Science.gov (United States)

    Negenborn, J; van der Marel, M C; Ganter, M; Steinhagen, D

    2015-06-12

    Cyprinid herpesvirus-3 (CyHV-3) causes a fatal disease in carp (Cyprinus carpio) and its ornamental koi varieties which seriously affects production and trade of this fish species globally. Up to now, the pathophysiology of this disease remains unclear. Affected individuals develop most prominent lesions in gills, skin and kidney, in tissues which are involved in the osmotic regulation of freshwater teleosts. Therefore, here serum and urine electrolyte levels were examined during the course of an experimental infection of carp with CyHV-3. In infected carp an interstitial nephritis with a progressive deterioration of nephric tubules developed, which was paralleled by elevated electrolyte losses, mainly Na(+) in the urine. The urine/plasma ratio for Na(+) increased from 0.03 in uninfected carp to 0.43-0.83 in carp under CyHV-3 infection, while concentration of divalent ions were not significantly changed. These electrolyte losses could not be compensated since plasma osmolality and Na(+) concentration dropped significantly in CyHV-3 infected carp. This was most probably caused by the progressive deterioration of the branchial epithelium, which in teleosts plays a prominent role in osmoregulation, and which was seen concomitantly with decreasing electrolyte levels in the serum of carp under CyHV-3 infection. Immediately after infection with CyHV-3, by day 2 post exposure, affected carp showed severe anaemia and prominent leucocytosis indicating the development of an acute inflammation, which could intensify the observed hydro-mineral imbalances. The data presented here show that an infection with CyHV-3 induces an acute inflammation and a severe dysfunction of osmoregulation in affected carp or koi, which may lead to death in particular in the case of acute disease progression. Copyright © 2015 Elsevier B.V. All rights reserved.

  5. Phylogeography, historical demography and distribution modelling of freshwater fishes inhabiting seasonally fluctuating Mediterranean river systems: a case study using the Iberian cyprinid Squalius valentinus

    Directory of Open Access Journals (Sweden)

    Silvia Perea

    2015-12-01

    Full Text Available The Mediterranean freshwater fish fauna has evolved under constraints imposed by the seasonal weather/hydrological patterns that define the Mediterranean climate. These conditions have influenced the genetic and demographic structure of aquatic communities since their origins in the Mid-Pliocene. Freshwater species in Mediterranean-type climates will likely constitute genetically well-differentiated populations as a consequence of fragmentation resulting from drought/flood cycles, to varying extents depending on basin size. We developed an integrative framework to study spatial patterns in genetic diversity, demographic trends, distribution modelling, and landscape genetics to evaluate the evolutionary response of Mediterranean-type freshwater fish to seasonal fluctuations in weather. To test this evolutionary response, the model species used was Squalius valentinus, an endemic cyprinid of the Spanish Levantine area, where seasonal weather fluctuations are extreme, although our findings may be extrapolated to other Mediterranean-type species. Our results underscore the significant role of the Mediterranean climate, along with Pleistocene glaciations, in diversification of S. valentinus. We found higher nuclear diversity in larger drainage basins, but higher mitochondrial diversity correlated to habitat suitability rather than basin size. We also found strong correlation between genetic structure and climatic factors associated with Mediterranean seasonality. Demographic and migration analyses suggested population expansion during glacial periods that also contributed to the current genetic structure of S. valentinus populations. The inferred species distribution models support the significant contribution of precipitation and isothermality for S. valentinus habitat suitability. We highlight the importance of stable habitat conditions, fostered by typical karstic springs found on the Mediterranean littoral coasts, for the preservation of

  6. The diet of otters ( Lutra lutra L.) in Danish freshwater habitats : comparisons of prey fish populations

    DEFF Research Database (Denmark)

    Taastrom, H.M.; Jacobsen, Lene

    1999-01-01

    Otter spraints from five Danish freshwater localities were analysed. In all localities fish was the main prey (76-99% of estimated bulk), especially in winter. Depending on locality, the prey fish mainly consisted of cyprinids (Cyprinidae), percids (Percidae) or salmonids (Salmonidae). Seasonal v...

  7. The barbs of Lake Tana, Ethiopia : morphological diversity and its implications for taxonomy, trophic resource partitioning, and fisheries

    NARCIS (Netherlands)

    Nagelkerke, L.

    1997-01-01

    The rediscovery of a unique species flock of cyprinid fish, its taxonomy and its feeding-biology are described. Fourteen species of barbs (Barbus spp, Cyprinidae, Teleostei) were found in highland (1800 m) Lake Tana, in northwestern Ethiopia. Lake Tana is

  8. Role of socio-cultural and economic factors in cyprinid fish distribution networks and consumption in Lawa Lake region, Northeast Thailand: Novel perspectives on Opisthorchis viverrini transmission dynamics.

    Science.gov (United States)

    Kim, Christina Sunyoung; Smith, John F; Suwannatrai, Apiporn; Echaubard, Pierre; Wilcox, Bruce; Kaewkes, Sasithorn; Sithithaworn, Paiboon; Sripa, Banchob

    2017-06-01

    Opisthorchis viverrini (Ov) is a fish-borne parasite endemic in parts of Lao PDR, Cambodia, southern Vietnam and Northeast Thailand (Isaan) where an estimated 10 million people are infected. Human Ov infection, associated with hepatobiliary complications, including cholangiocarcinoma (CCA), occurs when infected fish are consumed raw or undercooked, a longstanding cultural tradition in the region. This mixed- methods descriptive study was carried out in Isaan villages around Lawa Lake, Khon Kaen Province, known for their Ov endemicity. Focus group discussions (FGDs) and in depth interviews (IDIs) were used to explore socio-cultural determinants underlying raw fish consumption practices, and global positioning system (GPS) devices to map local fish distribution networks. Qualitative data affirmed major socio-cultural and dietary lifestyle transitions occurring consequent on recent decades of modernization policies and practices, but also the persistence of Isaan traditional raw-fish eating practices and incorrect beliefs about infection risk avoidance. Fish traders/middlemen purchase most of the catch at the lakeshore and play the dominant role in district market fish distribution networks, at least for the larger and less likely infected, fish species. The lower economic value of the small potentially-infected cyprinid fish means local fishermen typically distribute them free, or sell cheaply, to family and friends, effectively concentrating infection risk in already highly Ov infected villages. Our study confirmed the persistence of traditional Isaan raw-fish meal practices, despite major ongoing socio-cultural lifestyle transitions and decades of Ov infection health education programs. We contend that diffuse socio-cultural drivers underpin this practice, including its role as a valued cultural identity marker. A "fish economics" factor was also evident in the concentration of more likely infected fish back into local villages due to their low economic value at

  9. Effects of effects of suspended sediment on early-life stage survival of Yaqui chub, an endangered USA–Mexico borderlands cyprinid

    Science.gov (United States)

    Barkalow, Stephani L. Clark; Bonar, Scott A.

    2015-01-01

    High levels of total suspended sediment (TSS) can have negative consequences on fishes, such as altering food supply, lowering food acquisition, clogging gills, and disrupting reproduction. While effects of TSS on salmonids and estuarine fish are well studied, less is known about possible negative impacts of suspended sediment on desert fishes. Several imperiled desert fishes inhabit streams and springs near the U.S.–Mexico border and are potentially threatened by increased sediment loads from borderlands activity such as livestock grazing, road building, illegal traffic, and law enforcement patrols. One such species is the Yaqui Chub Gila purpurea, a federally listed endangered cyprinid. We exposed Yaqui Chub embryos and fry (mean TL = 12.6 mm; SE = 0.42) to a range of TSS levels commonly found in one of the only streams they inhabit, Black Draw, which crosses the Arizona–Mexico border. We tested effects of 0; 300; 500; 1,000; 5,000; and 10,000 mg/L TSS loads on fry and embryos over a 5-d period in three replicate containers for each treatment. Fifty percent hatch rate (i.e., median lethal concentration, LC50) was 3,977 mg/L for embryos. The LC50 for fry (concentration at which half died) was 8,372 mg/L after 12 h of exposure; however, after 5-d exposure, LC50 leveled at 1,197 mg/L. The TL of fry did not change significantly in any treatment over the 5-d period. Suspended sediment in Black Draw reached concentrations lethal to Yaqui Chub embryo and fry during four floods in 2012. Although some desert fishes have evolved in rivers and streams subject to elevated TSS and are tolerant to high TSS concentrations, other fish species are less tolerant and may be impacted by land practices which increase erosion into stream systems. Management of critically endangered desert fishes should include considerations of the effects of increased suspended sediment.

  10. Ancient and modern genome shuffling: Reticulate mito-nuclear phylogeny of four related allopatric species of Gyrodactylus von Nordmann, 1832 (Monogenea: Gyrodactylidae), ectoparasites on the Eurasian minnow Phoxinus phoxinus (L.) (Cyprinidae).

    Science.gov (United States)

    Lumme, Jaakko; Ziętara, Marek S; Lebedeva, Dar'ya

    2017-02-01

    Phylogenetic analyses including four allopatric species of Gyrodactylus von Nordmann, 1832 on the Eurasian minnow Phoxinus phoxinus (L.) (Cyprinidae) revealed incongruence between the nuclear ITS1-5.8S-ITS2 and mitochondrial cox1 phylogenies due to ancient hybridisation. Gyrodactylus pannonicus Molnár, 1968 was sampled close to its type-locality, the upper reaches of River Tisza, tributary of Danube in the Black Sea Basin. Faunistic search detected three new related species with maximum composite likelihood distances in cox1 between 16.8-23.2% (tentatively 1.3 to 1.8 My of divergence). Gyrodactylus albolacustris n. sp. recorded in the White Sea Basin, eastern Baltic Basin and Mongolia was close to G. pannonicus in the nuclear ITS (divergence of 0.9%), but diverged in cox1 by 19.8%. The Mongolian isolate of G. albolacustris n. sp. diverged from the European isolates in cox1 by 8.9%, suggesting 0.7 My of isolation. The two other new species differed from G. pannonicus by >4% in ITS and some large indels in ITS1, and by >20% in cox1. Gyrodactylus danastriae n. sp. was found in River Strwiąż, a tributary of the River Dniester (Black Sea Basin) and was characterised by smaller size of anchors and by 29-41 bp dimorphic insertion in ITS1. Gyrodactylus botnicus n. sp. is considered endemic in the Baltic Basin, but was also found in the White Sea Basin as a postglacial immigrant, where it had hybridised with G. albolacustris n. sp. in spite of the high divergence in ITS (3.9%) and cox1 (22%). The discordant nuclear and mitochondrial phylogenies revealed an ancient mitochondrial introgression: G. albolacustris n. sp. was derived from a hybridisation combining proto-pannonicus ITS with proto-danastriae mitochondria, perhaps 1.3 My ago. The postglacial hybridisation of G. albolacustris n. sp. (as the donor of mtDNA alb and ITS alb ) and G. botnicus n. sp. (donor of the ITS bot ) offered a model of shuffling of the genomic components: the process of the homogenisation

  11. Effects of carbon nanomaterials fullerene C{sub 60} and fullerol C{sub 60}(OH){sub 18-22} on gills of fish Cyprinus carpio (Cyprinidae) exposed to ultraviolet radiation

    Energy Technology Data Exchange (ETDEWEB)

    Socoowski Britto, Roberta; Longaray Garcia, Marcia; Martins da Rocha, Alessandra [Universidade Federal do Rio Grande - FURG, Instituto de Ciencias Biologicas, Campus Carreiros, Av. Italia km 8 s/n, Rio Grande, RS (Brazil); Programa de Pos Graduacao em Fisiologia Animal Comparada - Fisiologia Animal Comparada, FURG (Brazil); Artigas Flores, Juliana [Universidade Federal do Rio Grande - FURG, Instituto de Ciencias Biologicas, Campus Carreiros, Av. Italia km 8 s/n, Rio Grande, RS (Brazil); Pinheiro, Mauricio V. Brant [Departamento de Fisica, ICEx, Universidade Federal de Minas Gerais, MG (Brazil); Monserrat, Jose Maria [Universidade Federal do Rio Grande - FURG, Instituto de Ciencias Biologicas, Campus Carreiros, Av. Italia km 8 s/n, Rio Grande, RS (Brazil); Programa de Pos Graduacao em Fisiologia Animal Comparada - Fisiologia Animal Comparada, FURG (Brazil); Ribas Ferreira, Josencler L., E-mail: josenclerf@gmail.com [Universidade Federal do Rio Grande - FURG, Instituto de Ciencias Biologicas, Campus Carreiros, Av. Italia km 8 s/n, Rio Grande, RS (Brazil); Programa de Pos Graduacao em Fisiologia Animal Comparada - Fisiologia Animal Comparada, FURG (Brazil)

    2012-06-15

    In consequence of their growing use and demand, the inevitable environmental presence of nanomaterials (NMs) has raised concerns about their potential deleterious effects to aquatic environments. The carbon NM fullerene (C{sub 60}), which forms colloidal aggregates in water, and its water-soluble derivative fullerol (C{sub 60}(OH){sub 18-22}), which possesses antioxidant properties, are known to be photo-excited by ultraviolet (UV) or visible light. To investigate their potential hazards to aquatic organisms upon exposure to UV sunlight, this study analyzed (a) the in vitro behavior of fullerene and fullerol against peroxyl radicals (ROO{center_dot}) under UV-A radiation and (b) the effects of these photo-excited NMs on oxidative stress parameters in functional gills extracted from the fish Cyprinus carpio (Cyprinidae). The variables measured were the total antioxidant capacity, lipid peroxidation (TBARS), the activities of the antioxidant enzymes glutathione reductase (GR) and glutamate cysteine ligase (GCL), and the levels of the non-enzymatic antioxidant glutathione (GSH). The obtained results revealed the following: (1) both NMs behaved in vitro as antioxidants against ROO{center_dot} in the dark and as pro-oxidants in presence of UV-A, the latter effect being reversed by the addition of sodium azide, which is a singlet oxygen ({sup 1}O{sub 2}) quencher; (2) fullerene induced toxicity with or without UV-A incidence, with a significant (p < 0.05) increase in lipid peroxidation (with greater damage under illumination), a decrease in GCL activity, and the depletion of GSH stocks (under illumination), all of which were attributed to {sup 1}O{sub 2} generation; and (3) fullerol also decreased GCL activity and GSH formation (p < 0.05) but without lipid damage. The overall results show that fullerene can be toxic with or without light incidence, whereas UV radiation seems to play a key role in the environmental toxicity of carbon NMs through {sup 1}O{sub 2} formation.

  12. Establishment of a new cell line susceptible to Cyprinid herpesvirus 3 (CyHV-3) and possible latency of CyHV-3 by temperature shift in the cells.

    Science.gov (United States)

    Imajoh, M; Fujioka, H; Furusawa, K; Tamura, K; Yamasaki, K; Kurihara, S; Yamane, J; Kawai, K; Oshima, S

    2015-06-01

    A new cell line named CCF-K104 predominantly consisting of fibroblastic cells showed optimal growth at temperatures from 25 °C to 30 °C. Serial morphological changes in the cells induced by Cyprinid herpesvirus 3 (CyHV-3) included cytoplasmic vacuolar formation, cell rounding and detachment. Mature virions were purified from CyHV-3-infected CCF-K104 cells by sucrose gradient ultracentrifugation and had a typical herpesvirus structure on electron microscopy. Infectious CyHV-3 was produced stably in CCF-K104 cells over 30 viral passages. Our findings showed that CCF-K104 is a useful cell line for isolation and productive replication of CyHV-3. A temperature shift from 25 °C to 15 °C or 35 °C did not allow serial morphological changes as observed at 25 °C for 14 days. Under the same conditions, real-time PCR showed that CyHV-3 was present with low viral DNA loads, suggesting that CyHV-3 may establish latent infection in CCF-K104 cells. Amplification of the left and right terminal repeat sequences of the CyHV-3 genome arranged in a head-to-tail manner was detected by nested PCR following an upshift in temperature from 25 °C to 35 °C. The PCR results suggested that the circular genome may represent a latent form of CyHV-3. © 2014 John Wiley & Sons Ltd.

  13. Evaluation of PIT-tagging in cyprinids

    DEFF Research Database (Denmark)

    Skov, Christian; Brodersen, J.; Brönmark, C.

    2005-01-01

    Laboratory and field experiments were used to investigate how different marking procedures, with 23 mm PIT (passive integrated transponders) - tags. affected mortality, body condition and tag expulsion in small roach Rutilus rutilus and rudd Scardinus erythrophthalmus (117 to 163 mm total length...

  14. Cyprinid fishes: systematics, biology, and exploitation

    National Research Council Canada - National Science Library

    Winfield, Ian J; Nelson, Joseph S

    1991-01-01

    .... As this family is extremely important as forage fish for other animals, as food fish for humans and are commonly used as models in experimental work, the book will be of great interest to all those with an interest in freshwater fish, fish ecology, fisheries biology and aquaculture systems.

  15. Catchment-scale determinants of nonindigenous minnow richness in the eastern United States

    Science.gov (United States)

    Peoples, Brandon K.; Midway, Stephen R.; DeWeber, Jefferson T.; Wagner, Tyler

    2018-01-01

    Understanding the drivers of biological invasions is critical for preserving aquatic biodiversity. Stream fishes make excellent model taxa for examining mechanisms driving species introduction success because their distributions are naturally limited by catchment boundaries. In this study, we compared the relative importance of catchment-scale abiotic and biotic predictors of native and nonindigenous minnow (Cyprinidae) richness in 170 catchments throughout the eastern United States. We compared historic and contemporary cyprinid distributional data to determine catchment-wise native/nonindigenous status for 152 species. Catchment-scale model predictor variables described natural (elevation, precipitation, flow accumulation) and anthropogenic (developed land cover, number of dams) abiotic features, as well as native congener richness. Native congener richness may represent either biotic resistance via interspecific competition, or trait preadaptation according to Darwin's naturalisation hypothesis. We used generalised linear mixed models to examine evidence supporting the relative roles of abiotic and biotic predictors of cyprinid introduction success. Native congener richness was positively correlated with nonindigenous cyprinid richness and was the most important variable predicting nonindigenous cyprinid richness. Mean elevation had a weak positive effect, and effects of other abiotic factors were insignificant and less important. Our results suggest that at this spatial scale, trait preadaptation may be more important than intrageneric competition for determining richness of nonindigenous fishes.

  16. Evolutionary Trends of the Pharyngeal Dentition in Cypriniformes (Actinopterygii: Ostariophysi)

    OpenAIRE

    Pasco-Viel, Emmanuel; Charles, Cyril; Chevret, Pascale; Semon, Marie; Tafforeau, Paul; Viriot, Laurent; Laudet, Vincent

    2010-01-01

    Background : The fish order Cypriniformes is one of the most diverse ray-finned fish groups in the world with more than 3000 recognized species. Cypriniformes are characterized by a striking distribution of their dentition: namely the absence of oral teeth and presence of pharyngeal teeth on the last gill arch (fifth ceratobranchial). Despite this limited localisation, the diversity of tooth patterns in Cypriniformes is astonishing. Here we provide a further description of this diversity usin...

  17. Evolutionary trends of the pharyngeal dentition in Cypriniformes (Actinopterygii: Ostariophysi.

    Directory of Open Access Journals (Sweden)

    Emmanuel Pasco-Viel

    Full Text Available BACKGROUND: The fish order Cypriniformes is one of the most diverse ray-finned fish groups in the world with more than 3000 recognized species. Cypriniformes are characterized by a striking distribution of their dentition: namely the absence of oral teeth and presence of pharyngeal teeth on the last gill arch (fifth ceratobranchial. Despite this limited localisation, the diversity of tooth patterns in Cypriniformes is astonishing. Here we provide a further description of this diversity using X-ray microtomography and we map the resulting dental characters on a phylogenetic tree to explore evolutionary trends. RESULTS: We performed a pilot survey of dental formulae and individual tooth shapes in 34 adult species of Cypriniformes by X-ray microtomography (using either conventional X-ray machine, or synchrotron microtomography when necessary or by dissecting. By mapping morphological results in a phylogenetic tree, it emerges that the two super-families Cobitoidea and Cyprinoidea have followed two distinct evolutionary pathways. Furthermore, our analysis supports the hypothesis of a three-row dentition as ancestral for Cyprinoidea and a general trend in tooth row reduction in most derived lineages. Yet, this general scheme must be considered with caution as several events of tooth row gain and loss have occurred during evolutionary history of Cyprinoidea. SIGNIFICANCE: Dentition diversity in Cypriniformes constitutes an excellent model to study the evolution of complex morphological structures. This morphological survey clearly advocates for extending the use of X-ray microtomography to study tooth morphology in Cypriniformes. Yet, our survey also underlines that improved knowledge of Cypriniformes life traits, such as feeding habits, is required as current knowledge is not sufficient to conclude on the link between diet and dental morphology.

  18. Polypteridae (Actinopterygii: Cladistia) and DANA-SINEs insertions.

    Science.gov (United States)

    Morescalchi, Maria Alessandra; Barucca, Marco; Stingo, Vincenzo; Capriglione, Teresa

    2010-06-01

    SINE sequences are interspersed throughout virtually all eukaryotic genomes and greatly outnumber the other repetitive elements. These sequences are of increasing interest for phylogenetic studies because of their diagnostic power for establishing common ancestry among taxa, once properly characterized. We identified and characterized a peculiar family of composite tRNA-derived short interspersed SINEs, DANA-SINEs, associated with mutational activities in Danio rerio, in a group of species belonging to one of the most basal bony fish families, the Polypteridae, in order to investigate their own inner specific phylogenetic relationships. DANA sequences were identified, sequenced and then localized, by means of fluorescent in situ hybridization (FISH), in six Polypteridae species (Polypterus delhezi, P. ornatipinnis, P. palmas, P. buettikoferi P. senegalus and Erpetoichthys calabaricus) After cloning, the sequences obtained were aligned for phylogenetic analysis, comparing them with three Dipnoan lungfish species (Protopterus annectens, P. aethiopicus, Lepidosiren paradoxa), and Lethenteron reissneri (Petromyzontidae)was used as outgroup. The obtained overlapping MP, ML and NJ tree clustered together the species belonging to the two taxonomically different Osteichthyans groups: the Polypteridae, by one side, and the Protopteridae by the other, with the monotypic genus Erpetoichthys more distantly related to the Polypterus genus comprising three distinct groups: P. palmas and P. buettikoferi, P. delhezi and P. ornatipinnis and P. senegalus. In situ hybridization with DANA probes marked along the whole chromosome arms in the metaphases of all the Polypteridae species examined. Copyright © 2010 Elsevier B.V. All rights reserved.

  19. Morphological recognition of artificial F1 hybrids between three common European cyprinid species:Rutilus rutilus,Blicca bjoerkna and Abramis brama%三种普通欧洲鲤科鱼类(拟鲤、粗鳞鳊和欧鳊)人工杂交F1代形态学观察

    Institute of Scientific and Technical Information of China (English)

    B. NZAU MATONDO; M. OVIDIO; P. PONCIN; P. VANDEWALLE; J.C. PHILIPPART

    2008-01-01

    black line. In all interspecific crosses of these three cyprinid species fish, the reciprocal hybrids were generally indistinguishable.

  20. New circumscription of freshwater fish parasites Monobothrium diesing, 1863 and Promonobothrium mackiewicz, 1968 (Cestoda: Caryophyllidea) using morphological and molecular evidence.

    Science.gov (United States)

    Scholz, Tomáš; Oros, Mikuláš; Choudhury, Anindo; Brabec, Jan; Waeschenbach, Andrea

    2015-02-01

    Two genera of caryophyllidean cestodes, Monobothrium Diesing, 1863 and Promonobothrium Mackiewicz, 1968 , from cypriniform fishes (cyprinids and suckers) in the Holarctic Region, are revised using morphological and molecular evidence. Monobothrium, which includes morphologically distinct species that occur in European cyprinids (Cyprinidae) and North American suckers (Catostomidae), is separated into 3 genera. Monobothrium becomes monotypic and is represented by its type species, Monobothrium wageneri Nybelin, 1922 , which occurs in European tench, Tinca tinca (Cyprinidae). Monobothrium auriculatum Kulakovskaya, 1961 from Leuciscus danilevskii (Cyprinidae) in the Ukraine, is tentatively transferred to Caryophyllaeus Gmelin, 1790 as Caryophyllaeus auriculatus ( Kulakovskaya, 1961 ) n. comb. because it possesses morphological characteristics considered to be typical of Caryophyllaeus but which are absent in Monobothrium (shape of the scolex, presence of a seminal receptacle, short neck, and absence of a large, muscular papilla surrounding the large gonopores). The 5 remaining Monobothrium species from suckers in North America are transferred to Promonobothrium based on shared morphological features and molecular data. Species of Promonobothrium differ from M. wageneri by having an external seminal vesicle (absent in M. wageneri), the absence of postovarian vitelline follicles in North American species (present in M. wageneri), and a scolex that is digitiform papillate, loculopapillate, or loculotruncate, i.e., equipped with weak loculi and a terminal introvert (vs. claviform, bluntly ended, with 6 weak, shallow, longitudinal grooves in M. wageneri). Phylogenetic analysis of nuclear ribosomal RNA genes ssr- and lsrDNA placed Promonobothrium minytremi within a clade of 3 of the 5 North American Monobothrium species. The position of M. wageneri, although distinct from the remaining Monobothrium species, is unresolved within the Caryophyllidea. This study further

  1. THREATENED FISHES OF THE WORLD: Chondrostoma kinzelbachi KRUPP, 1985 (CYPRINIDAE

    Directory of Open Access Journals (Sweden)

    Gulnaz Özcan

    2013-01-01

    Full Text Available Orontes nase, Chondrostoma kinzelbachi is an endangered freshwater fish species in Turkey and Syria. Populations are declining due to dam construction and water pollution. Orontes nase populations should be completely protected.

  2. Mitochondrial diversity and phylogeography of Acrossocheilus paradoxus (Teleostei: Cyprinidae).

    Science.gov (United States)

    Ju, Yu-Min; Hsu, Kui-Ching; Yang, Jin-Quan; Wu, Jui-Hsien; Li, Shan; Wang, Wei-Kuang; Ding, Fang; Li, Jun; Lin, Hung-Du

    2018-01-31

    Mitochondrial DNA cytochrome b sequences (1141 bp) in 229 specimens of Acrossocheilus paradoxus from 26 populations were identified as four lineages. The pairwise genetic distances among these four lineages ranged from 1.57 to 2.37% (mean= 2.00%). Statistical dispersal-vicariance analysis suggests that the ancestral populations were distributed over mainland China and Northern and Western Taiwan. Approximate Bayesian computation approaches show that the three lineages in Taiwan originated from the lineage in mainland China through three colonization routes during two glaciations. The results indicated that during the glaciation and inter-glacial periods, the Taiwan Strait was exposed and sank, which contributed to the dispersion and differentiation of populations. Furthermore, the populations of A. paradoxus colonized Taiwan through a land bridge to the north of the Formosa Bank, and the Miaoli Plateau in Taiwan was an important barrier that limited gene exchange between populations on both the sides.

  3. Mahseers genera Tor and Neolissochilus (Teleostei: Cyprinidae) from southern Vietnam.

    Science.gov (United States)

    Hoàng, Huy Đức; Phạm, Hùng Mạnh; Durand, Jean-Dominique; Trần, Ngân Trọng; Phan, Phúc Đình

    2015-08-25

    Two new species and two new basin records of mahseers in the genera Tor and Neolissochilus are described from the upper Krong No and middle Đồng Nai drainages of the Langbiang Plateau in southern Vietnam. These new species and new records are known from streams and rivers in montane mixed pine and evergreen forests between 140 and 1112 m. Their populations are isolated in the Sre Pok River of the Mekong basin, the middle of the Đồng Nai basin, and the An Lão River. Both new species are differentiated from their congeners by a combination of the following characters: 23-24 lateral scales, 9-10 predorsal scales, 2/7 or 1/8 pelvic-fin rays, mouth position, median lobe of lower lip, rostral hood, colour in life and by divergent mitochondrial DNA. Tor mekongensis sp. nov. is differentiated from Tor dongnaiensis sp. nov. by the number of transverse scale rows (3/1/2 vs. 4/1/2), number of pelvic-fin rays (2/7 vs. 1/8), a blunt rostral hood vs. pointed, caudal-fin lobes that are equal vs. unequal, and by mitochondrial DNA (0.7% sequence divergence). Molecular evidence identifies both species as members of the genus Tor and distinct from all congeners sampled (uncorrected sequence divergences >1.9% for all Tor species for which homologous COI sequences are available). Tor sinensis is recorded in the Krong No and the Sre Pok rivers, further south of its known distribution. Polymorphism is described in Neolissochilus stracheyi with a Tor-like morph and a Neolissochilus-like morph.

  4. (Pisces, Cyprinidae) in Wuras Dam, a shallow, turbid impoundment

    African Journals Online (AJOL)

    tooname in liggaamsmassa. Suksesvolle voortplanting is afhanklik van geskikte vloedtoostande gedurende die brooiseisoen wat vanaf November tot Maart of April strek. Die vis het blykbaar in. Wurasdam gebrooi. AI die wyfies het nie gelyktydig kuitgeskiet nie en daar is aanduidings dat individue meer as een maal per sei-.

  5. (Pisces, Cyprinidae) from the eastern Cape Province, South Africa

    African Journals Online (AJOL)

    1990-03-05

    Mar 5, 1990 ... small, aligned in regular rows, indistinct on nape and embedded between pectoral fins and antero-ventral region. .... major row teeth of Barbus omfltolicus sp.nov. B - Posterior view of the right pharyngeal bone and teeth of B. onwtoiicus sp.nov. Scale bar is 1 mm. 191. Figure 4 The nuptial tubercles on the ...

  6. Occurrence of monogeneans on some cyprinid fishes from Murat ...

    African Journals Online (AJOL)

    use

    2011-12-12

    Dec 12, 2011 ... Syrian. One of them is D. alatus. Another study is about. D. microcirrus recorded in C. trutta in the north of Iraq. (Abdullah, 2009). G. rufa is a rarely studied fish in. Euphrates system and Persian Gulf Basin. Only Gussev et al. (1993) studied this fish and recorded D. rectotrabus and D. acinacus from River Dez ...

  7. Cadmium as toxicant in Freshwater Cyprinid, Labeo rohita

    African Journals Online (AJOL)

    Sajo

    2012-04-24

    Apr 24, 2012 ... urbanization, expansion of industrial activity, industrial wasteful effluents .... blood capillaries, and sinusoids were randomly distri- buted. ..... cholinesterase activity of freshwater fish, Oreochromis mossambicus. Peters. Asia.

  8. Seasonal and daily protandry in a cyprinid fish

    Czech Academy of Sciences Publication Activity Database

    Šmejkal, Marek; Ricard, Daniel; Vejřík, Lukáš; Mrkvička, Tomáš; Vebrová, Lucie; Baran, Roman; Blabolil, Petr; Sajdlová, Zuzana; Vejříková, Ivana; Prchalová, Marie; Kubečka, Jan

    2017-01-01

    Roč. 7, JUL (2017), č. článku 4737. ISSN 2045-2322 R&D Projects: GA MŠk(CZ) 7F14316; GA ČR GPP505/12/P647; GA MŠk(CZ) EE2.3.30.0032 Grant - others:AV ČR(CZ) StrategieAV21/9 Program:StrategieAV Institutional support: RVO:60077344 Keywords : sex ual selection * asp. * reproductive success * breeding areas * life-history Subject RIV: EH - Ecology, Behaviour OBOR OECD: Ecology Impact factor: 4.259, year: 2016

  9. Five newly recorded Cyprinid fish (Teleostei: Cypriniformes) in Myanmar

    Science.gov (United States)

    Qin, Tao; Chen, Zhi-Ying; Xu, Lu-Lu; Zaw, Paing; Kyaw, Yunn Mi Mi; Maung, Kyaw Win; Chen, Xiao-Yong

    2017-01-01

    Freshwater fish from the Putao and Myitkyina areas were collected in three ichthyofaunal surveys of the Mali Hka River and tributaries in and around Khakaborazi National Park and Hponkanrazi Wildlife Sanctuary, Kachin State, from 2014-2016. Tor yingjiangensis Chen et Yang 2004, Tor qiaojiensis Wu et al. 1977, Garra qiaojiensis Wu et al. 1977, Garra bispinosa Zhang 2005, and Schizothorax oligolepis Huang 1985, originally described from the upper Irrawaddy (Ayeyarwaddy) River in China, are first reported herein as new records to Myanmar. Counts, measurements, descriptions, photographs, and distributions of the specimens of the five newly recorded species are provided. PMID:29181904

  10. Sensory and chemical characteristics of sausages produced of cyprinid meat

    OpenAIRE

    Okanović, Đorđe G.; Ćirković, Miroslav A.; Novakov, Nikolina J.; Ljubojević, Dragana B.; Karan, Dragica D.; Matekalo-Sverak, Vesna F.; Mašić, Zoran S.

    2013-01-01

    Fish meat and fish products are valuable source of nutrients of great importance for diverse and healthy nutrition. The optimal ratio of protein, fat, carbohydrates, minerals and vitamins contribute to high nutritive value offish meat. Recommendations that fish should be regularly used in the diet are based on the fact that fish meat is the most important nutritional source of n-3 highly unsaturated fatty acids (n-3 HUFA). The aim of this study was to investigate sensory and chemical properti...

  11. [Species composition and geographical distribution of threatened fishes in Yunnan Province of Southwest China].

    Science.gov (United States)

    Zhang, Qian; Zhong, Jin-Xin

    2013-05-01

    Based on the related published papers, and by using Geographic Information System (ArcGIS 9.3), this paper analyzed the species composition and geographical distribution of threatened fishes in Yunnan Province of Southwest China. There were 83 threatened species living in the Province, belonging to 5 orders, 13 families, and 47 genera. Cypriniformes was absolutely dominant, with 64 species, followed by Siluriformes, with 16 species. Cyprinidae fishes had 51 species, accounting for 79.7% of Cypriniformes. The most species of Cyprinid fishes were of Barbinae (14 species), Cyprininae (10 species), and Cultrinae (10 species). The threatened fishes could be divided into two zoogeographical regions, i. e., Tibetan Plateau region and Oriental region, and their species composition and geographical distribution were resulted from the historical evolution adapted to the related environments. Whatever in rivers and in lakes, the Cyprinid fishes were both absolutely dominant, occupying 36.1% and 31.3% of the total, respectively. The Cyprinid fishes in rivers were mostly of endangered species, while those in lakes were mostly of vulnerable species. The factors affecting the threatened fishes in the Province were discussed from the two aspects of geodynamic evolution and present situation.

  12. Correlates of elemental-isotopic composition of stream fishes: the importance of land-use, species identity and body size.

    Science.gov (United States)

    Montaña, C G; Schalk, C M

    2018-04-01

    The isotopic (δ 13 C and δ 15 N) and stoichiometric (C:N:P) compositions of four fish species (Family Centrarchidae: Lepomis auritus, Lepomis cyanellus; Family Cyprinidae: Nocomis leptocephalus, Semotilus atromaculatus) were examined across four North Carolina Piedmont streams arrayed along an urbanization gradient. Both isotopic and stoichiometric composition of fishes appeared to track changes occurring in basal resource availability. Values of δ 13 C of basal resources and consumers were more enriched at the most urbanized streams. Similarly, basal resources and consumers were δ 15 N-enriched at more urbanized streams. Basal resource stoichiometry varied across streams, with periphyton being the most variable. Primary consumers stoichiometry also differed across streams. Intraspecific variation in fish stoichiometry correlated with the degree of urbanization, as the two cyprinids had higher N content and L. cyanellus had higher P content in more urbanized streams, probably due to enrichment of basal resources. Intrinsic factors, specifically species identity and body size also affected stoichiometric variation. Phosphorus (P) content increased significantly with body size in centrarchids, but not in cyprinids. These results suggest that although species identity and body size are important predictors of elemental stoichiometry, the complex nature of altered urban streams may yield imbalances in the elemental composition of consumers via their food resources. © 2018 The Fisheries Society of the British Isles.

  13. An Update on the Invasion of Weakfish Cynoscion regalis (Bloch & Schneider, 1801 (Actinopterygii: Sciaenidae into Europe

    Directory of Open Access Journals (Sweden)

    Pedro Morais

    2017-10-01

    Full Text Available New information on weakfish introduction vectors, its invasive status, distribution, and use as a fishing resource arose after the publication of “The transatlantic introduction of weakfish Cynoscion regalis (Bloch & Schneider, 1801 (Sciaenidae, Pisces into Europe” by Morais and Teodósio (2016. Currently, the first known report of weakfish in Europe dates back to September 2009, with a specimen captured in the Schelde estuary (Belgium/The Netherlands. This fact suggests that weakfish could have been introduced into Europe via multiple and independent ballast water introduction events, and not through a point-source introduction event with subsequent dispersion as previously hypothesized. It is also unlikely that Schelde weakfish migrated southwards to colonize Iberian aquatic ecosystems. Weakfish have established a population in the Gulf of Cádiz region and have already reached an invasive status in the Sado estuary (Portugal. Weakfish were also captured in several other locations along the Portuguese coast, including the Tagus and Mira estuaries at least since 2013 or 2014, and the Ria Formosa lagoon in 2017. Tagus anglers caught weakfish specimens of ~1 kg and ~40 cm in November 2016, which corresponds to fish of 3+ years of age in the native range. The presence of weakfish in the Tagus estuary is still fairly unknown to local anglers. Sado weakfish has already been sold in local fish markets in southern Portugal for 3 to 10 € kg−1. However, we consider that the weakfish sale price is underrated in comparison with other wild species (e.g., meagre, seabass, gilthead seabream. Increasing sale price will convince fishers to use weakfish as a new fishing resource; however, it is necessary to promote the species among consumers and evaluate consumers’ preference in respect to other species. A putative biological threat might turn into a new valuable fishing resource by implementing adequate management solutions.

  14. Distribution and size of the mojarra Diapterus rhombeus (Cuvier (Actinopterygii, Gerreidae in a Southeastern Brazilian bay

    Directory of Open Access Journals (Sweden)

    Marcus Rodrigues da Costa

    2012-06-01

    Full Text Available Diapterus rhombeus is one of the most abundant fish species in the Sepetiba bay, which is a 520 km² semi-closed coastal area in Southeastern Brazil. The size and distribution of this species have been described in order to assess the use of the area as a rearing ground in both spatial and temporal (seasonal dimensions. A fish sampling program was conducted monthly by using an otter trawl between October 1998 and September 1999, in three bay zones (outer, middle and inner, defined according to depth and salinity gradient. Highly significant differences were found for CPUEs (number and biomass among seasons and zones. Size ranged from 50 to 230 mm TL, and three size-groups were defined according to a maturation scale (n = 1435: 1 - immature ( 100 mm TL. Immature and L50 individuals were more abundant (number and biomass during Autumn in the inner zone, while adults (L100 predominated during Summer in the outer zone. Evidences of movements of young-of-the-year (50-90 mm TL individuals from the inner to the outer zone were detected as they reach larger sizes (180 mm TL in the second year of life. Condition (k was higher in larger sized individuals in the outer zone during Spring, when they are apt to start the reproductive process. Two cohorts were detected according to modal progression: the first (smaller size showing faster growth than the second, evidencing a slower growth rate as they reach larger size. Distinct size classes occupying different bay zones suggest that the age-groups optimize their coexistence by partitioning the available resources, avoiding intra-specific competition.Diapterus rhombeus é uma das espécies de peixes mais abundantes na Baia de Sepetiba, ambiente costeiro de 520 km² de área semifechada no sudoeste do Brasil. O tamanho e distribuição dos indivíduos desta espécie foram descritos com o objetivo de determinar o uso das áreas de criação numa dimensão espacial e temporal (sazonal. Um programa de amostragem de arrasto de fundo foi conduzido mensalmente entre outubro 1998 a setembro 1999, em três zonas da baía (externa, central, interna, definidas através de gradientes de profundidade e salinidade. Diferenças altamente significativas foram encontradas para CPUEs (número e biomassa entre estações do ano e zonas. O comprimento variou de 50 a 230 mm CT. Três grupos de tamanhos foram definidos de acordo com uma escala de maturação gonadal (n = 1435: 1 - imaturo ( 100 mm CT. Imaturos e indivíduos L50 foram abundantes (número e biomassa durante o outono na zona interna, enquanto adultos (L100 predominaram durante o verão na zona externa. Evidências de movimento de indivíduos jovens (50-90 mm CT da zona interna para zona externa foram detectadas conforme os animais foram atingindo maiores tamanhos (180 mm CT, no segundo ano de vida. A condição (k aumentou em indivíduos maiores na zona externa durante a primavera, quando estes se tornam aptos a iniciar o processo reprodutivo. Duas coortes foram detectadas de acordo com a progressão modal: a primeira (menores tamanhos mostrando crescimento mais rápido do que a segunda, evidenciando uma taxa menor de crescimento a medida que atingem maiores tamanhos. Diferentes classes de comprimento ocupando diferentes zonas da baía sugerem que grupos de idades otimizam sua coexistência compartilhando os recursos disponíveis e evitando competição intra-especifica.

  15. Isopods (Isopoda: Aegidae, Cymothoidae, Gnathiidae associated with Venezuelan marine fishes (Elasmobranchii, Actinopterygii

    Directory of Open Access Journals (Sweden)

    Lucy Bunkley-Williams

    2006-12-01

    Full Text Available The parasitic isopod fauna of fishes in the southern Caribbean is poorly known. In examinations of 12 639 specimens of 187 species of Venezuelan fishes, the authors found 10 species in three families of isopods (Gnathiids, Gnathia spp. from Diplectrum radiale *, Heteropriacanthus cruentatus *, Orthopristis ruber * and Trachinotus carolinus *; two aegids, Rocinela signata from Dasyatis guttata *, H. cruentatus *, Haemulon aurolineatum *, H. steindachneri * and O. ruber ; and Rocinela sp. from Epinephelus flavolimbatus *; five cymothoids: Anilocra haemuli from Haemulon boschmae *, H. flavolineatum * and H. steindachneri *; Anilocra cf haemuli from Heteropriacanthus cruentatus *; Haemulon bonariense*, O. ruber*, Cymothoa excisa in H. cruentatus *; Cymothoa oestrum in Chloroscombrus chrysurus, H. cruentatus* and Priacanthus arenatus ; Cymothoa sp. in O. ruber; Livoneca sp. from H. cruentatus *; and Nerocila fluviatilis from H. cruentatus * and P. arenatus *. The Rocinela sp. and A. cf haemuli in the southern Caribbean could represent new species. The abundance of A. cf haemuli appears to have drastically reduced from 1994 to 1999 in the Gulf of Cariaco. The Cymothoa sp. represents an undescribed species that is apparently host specific to O. ruber . It does not occur in the Gulf of Cariaco, but is relatively abundant on the Caribbean coast of Sucre State, Venezuela. The Livoneca sp. is an undescribed species host specific to Diapterus rhombeus, Cymothoa excisa and C. oestrum were thought to have distinct host preferences, but both infected the Heteropriacanthus cruentatus in the present study.Gnathia spp. are reported from Venezuelan waters for the first time. Twenty new host records* are noted. The fish-associated isopod fauna is much more extensive and important than has previously been suspected. Rev. Biol. Trop. 54 (Suppl. 3: 175-188. Epub 2007 Jan. 15.Se conoce muy poco acerca de la fauna de isópodos parásitos de peces en el Caribe Sur. Tras examinar 12 639 especímenes de 187 especies de peces de Venezuela, los autores encontraron 10 especies de isópodos distribuidos en tres familias. Los asteriscos indican nuevos registros (Gnátidos, Gnathia spp. en Diplectrum radiale*, Heteropriacanthus cruentatus*; Orthopristis ruber* y Trachinotus carolinus*; dos aégidos, Rocinela signata en Dasyatis guttata*, H. cruentatus*, Haemulon aurolineatum*, H. steindachneri * y O. ruber ; y Rocinela sp. en Epinephelus flavolimbatus*; cinco cimotoidos: Anilocra haemuli en Haemulon boschmae*, H. flavolineatum* y H.steindachneri*; Anilocra cf haemuli en Heteropriacanthus cruentatus*; Haemulon bonariense*, O. ruber*; Cymothoa excise in H. cruentatus*; Cymothoa oestrum en Chloroscombrus chrysurus, H. cruentatus* y Priacanthus arenatus; Cymothoa sp. en O. ruber; Livoneca sp. en H. cruentatus*; y Nerocila fluviatilis en H. cruentatus* y P. arenatus*. Las especies Rocinela sp.y A.cf haemuli en el Caribe sur podrían representar especies distintas. La abundancia de A. cf haemuli en el Golfo de Coriaco parece haberse reducido drásticamente entre 1994 a 1999. Cymothoa sp.es una especie no descrita que aparentemente parasita específicamente a O. ruber. No se le encuentra en el Golfo de Cariaco, pero es relativamente abundante en la costa Caribe del Estado de Sucre, Venezuela. Livoneca sp. (especie sin describir parasita específicamente a Diapterus rhombeus. Anteriormente se pensaba que Cymothoa excisa y C. oestrum tenían distintas preferencias de hospedero, pero ambas infectan a Heteropriacanthus cruentatus. Gnathia spp. Son además primer registro en aguas venezolanas. Se informan en total 20 nuevos registros. La fauna de isópodos asociados a peces es mucho más extensa e importante de lo que se sospechaba.

  16. Distribution and size of the mojarra Diapterus rhombeus (Cuvier) (Actinopterygii, Gerreidae) in a Southeastern Brazilian bay

    OpenAIRE

    Costa,Marcus Rodrigues da; Albieri,Rafael Jardim; Neves,Leonardo Mitrano; Santos,Alex Braz Lacone; Araujo,Francisco Gerson

    2012-01-01

    Diapterus rhombeus is one of the most abundant fish species in the Sepetiba bay, which is a 520 km² semi-closed coastal area in Southeastern Brazil. The size and distribution of this species have been described in order to assess the use of the area as a rearing ground in both spatial and temporal (seasonal) dimensions. A fish sampling program was conducted monthly by using an otter trawl between October 1998 and September 1999, in three bay zones (outer, middle and inner), defined according ...

  17. Astyanax intermedius Eigenmann, 1908 (Actinopterygii: Characiformes: Characidae): Distribution extension in eastern Brazil

    OpenAIRE

    Lezama, Antônio; Triques, Mauro; Queiroz, Federico

    2011-01-01

    Astyanax intermedius is known to occur at Paraíba do Sul River basin and coastal rivers of Rio de Janeiro state. A detailed study is presented in order to clearly assign the species to the Doce River basin, in Minas Gerais state.

  18. A new species of dwarf sea bass, genus Serranus (Serranidae: Actinopterygii, from the southwestern Atlantic Ocean

    Directory of Open Access Journals (Sweden)

    Alfredo Carvalho Filho

    Full Text Available Serranus aliceae n. sp. is described from Rio de Janeiro and Espírito Santo States, southeastern Brazilian coast. The species is readily distinguished from its congeners by the following combination of characters: scales around the caudal peduncle 20 or 21; total gill rakers on first branchial arch 23-29; cheek-scales rows 5 or 6; and by the coloration of live specimens, which are reddish with a broad, conspicuous, white stripe from post-orbital region to the caudal-fin base and a white abdomen. The new species apparently inhabits the deeper (30 or more meters portions of reef environments along the subtropical Brazilian coast, and is possibly associated with upwelling cold-water masses. The species was observed hovering above rocky bottoms, feeding on micro-crustaceans, squid larvae, and zooplankton. This new species increases to eight the number of valid Serranus species recorded in the southwestern Atlantic.

  19. The parasite community of gobiid fishes (Actinopterygii: Gobiidae) from the Lower Volga River region

    Czech Academy of Sciences Publication Activity Database

    Kvach, Yuriy; Boldyrev, V.; Lohner, R.; Stepien, C. A.

    2015-01-01

    Roč. 70, č. 7 (2015), s. 948-957 ISSN 0006-3088 Institutional support: RVO:68081766 Keywords : Gobiidae * parasites * Volga River * Caspian Sea * introduced species Subject RIV: EG - Zoology Impact factor: 0.719, year: 2015

  20. Intraspecific ecomorphological variations in Poecilia reticulata (Actinopterygii, Cyprinodontiformes: comparing populations of distinct environments

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    Fábio T. Mise

    2015-06-01

    Full Text Available ABSTRACT Morphological variations, according to the principles of ecomorphology, can be related to different aspects of the organism way of life, such as occupation of habitats and feeding behavior. The present study sought to examine the intraspecific variation in two populations of Poecilia reticulata Peters, 1859, that occur in two types of environments, a lotic (Maringá Stream and a lentic (Jaboti Lake. Due to a marked sexual dimorphism, males and females were analyzed separately. Thus, the proposed hypotheses were that the populations that occur in distinct environments present morphological differences. The morphological variables were obtained using morphometric measurements and the ecomorphological indexes. The data were summarized in a Principal Component Analysis (PCA. A Multivariate Analysis of Variance (Manova was made to verify significant differences in morphology between the populations. Males and females showed similar ecomorphological patterns according to the environment they occur. In general the population from Maringá Stream had fins with major areas, and the Jaboti Lake population eyes located more dorsally. Additionally, others morphological differences such as wider mouth of the males from Maringá Stream, wider heads on Jaboti Lake females and more protractible mouths on males from Jaboti Lake suggest a set of environmental variables that can possibly influence the ecomorphological patterns of the populations, as the water current, availability of food resources and predation. In summary, the initial hypotheses could be confirmed, evidencing the occurrence of distinct ecomorphotypes in the same species according to the environment type.

  1. Population ecology and habitat preferences of juvenile flounder Platichthys flesus (Actinopterygii: Pleuronectidae) in a temperate estuary

    Science.gov (United States)

    Souza, Allan T.; Dias, Ester; Nogueira, Ana; Campos, Joana; Marques, João C.; Martins, Irene

    2013-05-01

    The European flounder Platichthys flesus is a widely distributed epibenthic species and an important component of demersal fish assemblages in the European Atlantic coastal waters. In Portuguese estuaries, this species reaches high densities, especially in Minho estuary (NW Iberian Peninsula, Europe), potentially playing an important role in the system's ecology. In this context, the population structure, production and the habitat use of juvenile P. flesus were investigated. Sampling took place monthly, from February 2009 until July 2010 along the entire estuarine gradient (5 sampling stations distributed in the first 29 km from the river mouth, with S1 located near the river mouth, S2 inside a salt marsh, S3 in a salinity transition zone, while S4 and S5 were located in the upper estuary). Flounder's density varied significantly among sampling stations and seasons (two-way PERMANOVA: p PERMANOVA: p < 0.001), with larger fishes being found in S1 during the autumn (168.50 ± 59.50 mm) and the smallest in S4 during the spring (33.80 ± 3.12 mm). Size classes associated differently with environmental variables, with larger juveniles being more abundant in the downstream areas of the estuary, whereas smaller juveniles were related to higher water temperatures, suggesting a habitat segregation of P. flesus of different sizes. The fish condition of P. flesus in Minho estuary was higher than in other systems, probably due to the dominance of juveniles on the population. Also, the densities found in this estuary were up to 32 times higher than in other locations, suggesting that Minho estuary is an important nursery area for the species. The estimated secondary production of P. flesus was lower than previous studies acknowledged in the system (0.037 g.WWm- 2.year- 1), indicating that the production estimates of this species in estuaries can vary considerably depending on of several factors such as the sampling year and strategy, population and fish size.

  2. West Pike Story: a dated phylogeography of Esox spp. (Actinopterygii, Esocidae

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    Gael P. J. Denys

    2015-11-01

    The results distinguish the 3 pike species as well as the 3 lineages of E. lucius from Skog et al. (2014. The presence of individuals from the South-European and circumpolar lineages might result from stocking operations. Finally, molecular dating with reanalysis old fossil calibrations places the European pikes diversification during the late Miocene (E. aquitanicus vs. the others, the late Pliocene (E. cisalpinus vs. E. lucius, and the Pleistocene ice ages (E. lucius lineages. Pike restocking should take into account the genetics of the fish in order to preserve these species with high economical and patrimonial value.

  3. A new saurichthyiform (Actinopterygii with a crushing feeding mechanism from the Middle Triassic of Guizhou (China.

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    Feixiang Wu

    Full Text Available Equipped with an effective predatory feeding mechanism enhanced by large and sharp teeth, pointed snout and elongate body, saurichthyiform fishes are considered common fish-eaters in the early Mesozoic aquatic ecosystems. Additionally, because of the similar body plan across species, saurichthyiforms are also regarded evolutionally conservative, with few morphological and ecological changes during their long history. However, their phylogenetic affinity remains unclear as to whether they are chondrostean, neopterygian or stem-actinopteran, and likewise the intrarelationships of the group have rarely been explored.Here we report a new saurichthyiform from the Middle Triassic of Guizhou, China, based on the well-preserved specimens including a 3-D braincase. The new taxon, Yelangichthys macrocephalus gen. et sp. nov., is unique among saurichthyiforms in having a peculiar neurocranium with a broad orbital tectum, paired posterior myodomes, a deep, transverse fossa in the posterodorsal part of the orbit, and a feeding mechanism structured for durophagy. Phylogenetic analysis places Yelangichthys gen. nov. at the most basal position in the Saurichthyiformes as the sister to Saurichthyidae, and a new family Yelangichthyidae is erected to include only Y. macrocephalus gen. et sp. nov. The monophyly of the Chondrostei comprising [Saurichthyiformes + Acipenseriformes] Birgeriiformes is supported, but not the monophyly of Saurichthys, the type genus of Saurichthyidae. With its outstanding osteological details, Yelangichthys gen. nov. greatly increases the neurocranial variations in saurichthyiforms, and its novel feeding structure suggests the consumption of hard-preys instead of fishes.Our findings highlight the detailed osteology of a saurichthyiform braincase and its feeding design. We suggest that saurichthyiforms are closely allied to the Acipenseriformes. Saurichthyiforms were very diverse in the cranial osteology and they might have undergone a rapid evolutionary radiation via, for the new material here, transforming the feeding mechanism and thus exploiting the food resources unsuitable for other saurichthyiforms.

  4. A New Saurichthyiform (Actinopterygii) with a Crushing Feeding Mechanism from the Middle Triassic of Guizhou (China)

    Science.gov (United States)

    Wu, Feixiang; Chang, Mee-mann; Sun, Yuanlin; Xu, Guanghui

    2013-01-01

    Background Equipped with an effective predatory feeding mechanism enhanced by large and sharp teeth, pointed snout and elongate body, saurichthyiform fishes are considered common fish-eaters in the early Mesozoic aquatic ecosystems. Additionally, because of the similar body plan across species, saurichthyiforms are also regarded evolutionally conservative, with few morphological and ecological changes during their long history. However, their phylogenetic affinity remains unclear as to whether they are chondrostean, neopterygian or stem-actinopteran, and likewise the intrarelationships of the group have rarely been explored. Methodology/Principal Findings Here we report a new saurichthyiform from the Middle Triassic of Guizhou, China, based on the well-preserved specimens including a 3-D braincase. The new taxon, Yelangichthys macrocephalus gen. et sp. nov., is unique among saurichthyiforms in having a peculiar neurocranium with a broad orbital tectum, paired posterior myodomes, a deep, transverse fossa in the posterodorsal part of the orbit, and a feeding mechanism structured for durophagy. Phylogenetic analysis places Yelangichthys gen. nov. at the most basal position in the Saurichthyiformes as the sister to Saurichthyidae, and a new family Yelangichthyidae is erected to include only Y. macrocephalus gen. et sp. nov. The monophyly of the Chondrostei comprising [Saurichthyiformes + Acipenseriformes] Birgeriiformes is supported, but not the monophyly of Saurichthys, the type genus of Saurichthyidae. With its outstanding osteological details, Yelangichthys gen. nov. greatly increases the neurocranial variations in saurichthyiforms, and its novel feeding structure suggests the consumption of hard-preys instead of fishes. Conclusions/Significance Our findings highlight the detailed osteology of a saurichthyiform braincase and its feeding design. We suggest that saurichthyiforms are closely allied to the Acipenseriformes. Saurichthyiforms were very diverse in the cranial osteology and they might have undergone a rapid evolutionary radiation via, for the new material here, transforming the feeding mechanism and thus exploiting the food resources unsuitable for other saurichthyiforms. PMID:24324657

  5. A new pachyrhizodontid fish (Actinopterygii, Teleostei from the Muhi Quarry (Albian-Cenomanian, Hidalgo, Mexico

    Directory of Open Access Journals (Sweden)

    G. Arratia

    2018-03-01

    Full Text Available A new genus and species – Motlayoichthys sergioi (ZooBank registration: urn:lsid:zoobank.org:pub:2C503741-2362-4234-8CE0-BB7D8BE5A236, urn:lsid:zoobank.org:act:EF5040FD-F306-4C0F-B9DA-2CC696CA349D – from the Cretaceous (Albian-Cenomanian of the Muhi Quarry, Hidalgo, central Mexico is assigned to the family Pachyrhizodontidae based on the presence of the inner premaxillary tooth and a few other cranial characters. Its unique suite of characters, that includes several autapomorphies supports its assignment as a new genus and species, such as the presence of a triangular head with three sides of similar lengths and with its deepest part at the level of the supraoccipital region; the lower jaw projecting anterior to the premaxilla; the ornamentation of cranial bones – especially those of the cheek region – consisting of fine longitudinal crests densely covering the bony surfaces; one or a few deep grooves on the mandibular teeth, which in addition bear acrodin tips ornamented with fine parallel striae; and the position of the dorsal fin, near the posterior margin of the cranium. Motlayoichthys n. gen., together with Michin, Pachyrhizodus, Rhacolepis, and Notelops, is part of the early radiation of pachyrhizodontoids during Albian times.

  6. Using mariculture as a breeding site: reproduction of Hypleurochilus fissicornis (Actinopterygii: Blenniidae

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    Bianca Possamai

    2015-09-01

    Full Text Available Mariculture in estuaries provides substrate for colonization by fouling organisms, thus attracting small cryptic fish species hitherto unknown in this environment. The blenny Hypleurochilus fissicornis is one of the species that is associated with this new system and is found in high abundance in bivalve farming. To understand the reproductive strategy that this species uses in this new environment, we collected specimens monthly in a mariculture on the southern coast of Paraná State (Brazil. After obtaining morphometric data, we removed gonads to determine sex and maturity stage. Gonads were weighed and analysed histologically. Oogenesis showed the same pattern as in other teleosts, but spermatogenesis showed a very complex dynamics. The spawning is multiple and synchronous between sexes, lasting eight months (May to December and peaking in winter. Hypleurochilus fissicornis was reproductively successful using the mariculture as a breeding site. The species has a variety of tactics to protect its offspring (e.g. batch spawning, long reproductive period, reduced L50, parental care and a reproductive peak in winter.

  7. A NEW SAURICHTHYS (ACTINOPTERYGII FROM THE SPATHIAN (EARLY TRIASSIC OF CHAOHU (ANHUI PROVINCE, CHINA

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    ANDREA TINTORI

    2014-07-01

    Full Text Available A new species of Saurichthys, S. majiashanensis , is described. It comes from a series of marine vertebrate-bearing beds of the upper member of the Nanlinghu Formation outcropping in the large quarry at the top of Majiashan (Majia Hill near Chaohu (Hefei City, Anhui Province, China. Its age is Middle Spathian (Olenekian, Early Triassic. This new species deeply differs from the several pre-Spathian species of Saurichthys mainly for having only two longitudinal scale rows together with a reduced grid-like structure for the neural elements in the vertebral column. Further derived characters are in the endoskeleton of the dorsal and anal fin, where radials articulate only to anterior lepidotrichia, the posterior ones being supported by the first scale from the caudal pedicle mid-dorsal and mid-ventral rows, deeply imbedded in the body. In addition, the haemal spines of the caudal region are much enlarged and reversed, with their distal parts pointing forwards. Though the skull is lacking, postcranial characters are enough to justify the erection of a new species. This new Saurichthys species, together with other few actinopterygians, can be considered as the oldest assemblage of the Triassic Middle Fish Fauna, which bloomed probably in the early Anisian and widespread especially all over the Tethys for the Middle Triassic and at least the Carnian in the Late Triassic. This new fish assemblage, together with some of the oldest marine reptiles, is considered as the beginning of the actual Triassic recovery among marine vertebrates.

  8. A new species of the genus Salaria Forsskål, 1775 (Actinopterygii, Blennidae in Morocco

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    Yahyaoui, A.

    2011-12-01

    Full Text Available The North African freshwater fish fauna is scarce compared to other regions of the world, probably due to historical and climatic factors. The western part of North Africa is more diverse than central or eastern areas. Populations of freshwater blennids that have been traditionally ascribed to the species Salaria fluviatilis nowadays inhabit the Sebou basin in Morocco. In this study we demonstrated morphologically and molecularly that these Moroccan populations constitute a new species, for which we provide a formal description. The following diagnostic characters distinguish the newly described species from other freshwater blennids: head without diagonal rows of dark dots or blotches on cheeks; supra-ocular tentacle thick and simple (not branched; 13-15 teeth on the upper jaw and 14-16 on the lower jaw; 16-17 soft anal fin rays; short pre-orbital distance (cephalic index: 2.9-3.8; height of the anterior (spiny part of the dorsal fin short (dorsal fin index: 0.8-1.3 and 34 vertebrae. Mitochondrial and nuclear genetic distances between the new Salaria species and either S. economidisi or S. fluviatilis are high, especially in the mitochondrial control region (18%, while these genetic distances range from 2 to 3% when the nuclear S7 marker is compared. These morphological and molecular characters differentiate the new species from the remaining freshwater species of the genus (Salaria economidisi and S. fluviatilis. The new species is confined to a small restricted area of the Sebou basin in Morocco and should be considered endangered (EN according to the IUCN Red List.La fauna de peces de agua dulce del norte de África es escasa comparada con otras regiones del mundo debido fundamentalmente a factores históricos y climáticos. El oeste del norte de África es más diverso que las regiones del centro y del este. En la cuenca del río Sebou habitan actualmente poblaciones de blenios de agua dulce que han sido tradicionalmente adscritas a la especie Salaria fluviatilis. En este estudio demostramos morfológica y molecularmente que estas poblaciones marroquíes constituyen una nueva especie, para la cual proveemos una descripción formal. Los siguientes caracteres diagnósticos distinguen a la nueva especie descrita del resto de especies de blenios de agua dulce: cabeza sin una fila diagonal de puntos oscuros o manchas en las mejillas; tentáculo supraocular grueso y simple (no ramificado; 13-15 dientes en la mandíbula superior y 14-16 en la inferior; 16-17 radios blandos en la aleta anal; distancia preorbital corta (índice cefálico: 2.9-3.8; altura de la region anterior de la aleta dorsal corta (índice dorsal: 0.8-1.3 y 34 vértebras. Las distancias genéticas mitocondriales y nuclear entre la nueva especie de Salaria y las otras dos especies son altas, especialmente en la región control (18%, mientras que estas distancias genéticas variaron entre 2-3% para el gen nuclear S7. Estos caracteres morfológicos y moleculares diferencian a la nueva especie del resto de blenios de agua dulce del género (Salaria fluviatilis y S. economidisi. La nueva especie está confinada a la cuenca del río Sebou en Marruecos y debería ser considerada como En Peligro (EN de acuerdo a las categorías de la lista roja.

  9. Fish, Actinopterygii, Perciformes, Gobiidae, Amblyeleotris rubrimarginata Mohlmann and Randall, 2002: range gap filled in northern Borneo

    OpenAIRE

    Townsend, Ditch

    2010-01-01

    The present work reports the shrimp goby Amblyeleotris rubrimarginata Mohlmann and Randall, 2002 in coral sand habitats off three different islands within the Tunku Abdul Rahman marine Park (TARP) off western Sabah in northern Borneo.

  10. Preliminary list of endangered fish species (Pisces, Elasmobranchii , Actinopterygii) in Brazil

    OpenAIRE

    Rosa, Ricardo S; Menezes, Naércio A

    1996-01-01

    The Brazilian fish fauna is still poorly known with respect to its diversity and conservation status, particularly of freshwater species. Human activities and population growth are rapidly impacting this fauna to an unchecked extent, so that many fish species are presently threatened. Also, some areas have been so critically altered that their fish fauna can no longer be properly inventoried. There is an urgent need to evaluate the conservation status of Brazilian fish species, since none are...

  11. Testicular maturation of Oligosarcus hepsetus (Cuvier (Actinopterygii, Characidae in a brazilian tropical reservoir

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    R. N. Santos

    Full Text Available Six reproductive classes of male Oligosarcus hepsetus (Cuvier, 1829, a medium-sized carnivorous Characiform species, are described based on macroscopic and histological techniques. A total of 175 individuals were caught monthly between April 2001 and June 2002 in the Lajes Reservoir, Brazil, one of the largest impoundment areas in the state of Rio de Janeiro. The reproductive classes were based upon changes in the testicular morphology and stages of germinative cells, i.e., resting, early maturing, late maturing, mature, partially spent and totally spent. Fish in the resting class showed testes with spermatogonia and spermatocytes along the wall of seminal lobules, while spermatids were present in the lumina of the lobules. During early maturing, active spermatogenesis occurs throughout the testis; in the late maturing and mature classes, the lobules are swollen with sperm that are typical of fish in breeding condition. Spent testes presented seminal lobules with residual spermatozoa, coinciding with decreasing GSI and greatly reduced sperm production. Overall, the testicular morphology and class of maturity development of O. hepsetus in the Lajes reservoir did not differ significantly from those of closely related species in other lentic environments. Lower GSI values in the oligotrophic Lajes reservoir than in other eutrophic natural lakes suggest that this species may be modifying this aspect of its reproductive strategy in response to the artificial environment.

  12. The South American freshwater fish Prochilodus lineatus (Actinopterygii: Characiformes: Prochilodontidae): new species in Vietnamese aquaculture

    Czech Academy of Sciences Publication Activity Database

    Kalous, L.; Bui, A.T.; Petrtýl, M.; Bohlen, Jörg; Chaloupková, P.

    2012-01-01

    Roč. 43, č. 6 (2012), 955-958 ISSN 1355-557X Grant - others:MZe(CZ) 29/MZe/B/08-10 Institutional research plan: CEZ:AV0Z50450515 Keywords : exotic species * aquaculture * Prochilodus lineatus Subject RIV: GL - Fishing Impact factor: 1.422, year: 2012

  13. Mitochondrial DNA reveals unexpected diversity of chubs (genus Squalius; Cypriniformes, Actinopterygii in the Adriatic basin

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    Ivana Buj

    2015-12-01

    Full Text Available The genus Squalius comprises more than 40 species inhabiting various freshwater habitats. They are distributed in Europe and Asia, with particularly high diversity recorded in the Mediterranean area. The taxonomic status of many populations is still matter of debate. With this investigation we aimed to help in resolving taxonomic uncertainties of the chubs distributed in the Adriatic basin in Croatia and Bosnia and Herzegovina. Phylogenetic reconstruction based on mitochondrial gene for cytochrome b revealed high diversity of chubs in the investigated area. Two evolutionary independent lineages are revealed: the first one comprising species Sq. svallize, Sq. tenellus, Sq. illyricus and Sq. zrmanjae; whereas the second lineage corresponds with Sq. squalus. High intraspecific structuring of Sq. squalus was detected, implying necessity of taxonomic revision of that species. Based on the obtained results, most important aspects of the evolutionary history of the genus Squalius in the Adriatic basin will be discussed and evolutionary significant units identified.

  14. Morphological development and allometric growth patterns of Acipenser persicus Borodin, 1897 (Actinopterygii, Acipenseridae during early development

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    Soheil Eagderi

    2017-06-01

    Full Text Available Morphological development and allometric growth patterns of reared Persian sturgeon, Acipenser persicus, were studied from hatching to 50 days post-hatching (dph. The larvae were sampled, their left sides photographed and seven morphometric characters, including total length, head length, tail length, trunk length, snout length, caudal peduncle and predorsal length were measured. Allometric growth patterns were calculated as a power function of total length and described using the growth coefficient to find important steps in early life history. The total length of the newly hatched larvae and fry were 10.59±0.8 and 38.8±2.9 mm at 1 and 50 dph, respectively. Morphogenesis and differentiation were the highest rates during the first 11 days of early development, i.e. endogenous feeding period. There were higher growth rate of head, snout and tail regions compared with those of other organs from the hatch up to yolk sac absorption, followed by positive or almost isometric patterns, after the begin of exogenous feeding, showing priority to enhance the feeding and swimming capabilities. This study confirmed that most of morphological changes of this species are occurred from hatching until the onset of exogenous feeding i.e. during the lecithotrophic phase.

  15. Fine structure and development of the collar enamel in gars, Lepisosteus oculatus, Actinopterygii

    Science.gov (United States)

    Sasagawa, Ichiro; Ishiyama, Mikio; Yokosuka, Hiroyuki; Mikami, Masato

    2008-06-01

    The fine structure of collar enamel and the cells constituting the enamel organ during amelogenesis in Lepisosteus oculatus was observed by light, scanning electron and transmission electron microscopy. In the enamel, slender crystals were arranged perpendicular to the surface and the stripes that were parallel to the surface were observed, suggesting that the enamel in Lepisosteus shares common morphological features with that in sarcopterygian fish and amphibians. Ameloblasts containing developed Golgi apparatus, rough endoplasmic reticulum (rER) and secretory granules were found in the secretory stage. In the maturation stage, a ruffled border was not seen at the distal end of the ameloblasts, while many mitochondria and lysosome-like granules were obvious in the distal cytoplasm. The enamel organ consisted of the outer dental epithelial cells, stratum reticulum cells and ameloblasts, but there was no stratum intermedium. It is likely that the ameloblasts have less absorptive function in comparison with the inner dental epithelial cells facing cap enameloid.

  16. Reproductive strategies of two sympatric "small barbs" (Barbus humilis and B. tanapelagius, Cyprinidae) in Lake Tana

    NARCIS (Netherlands)

    Dejen, E.; Sibbing, F.A.; Vijverberg, J.

    2003-01-01

    The reproductive strategies of two species of 'small barbs' (12,000 fish). Both species have a long breeding period (from March to September), and the distinct bimodal size-frequency distributions of eggs suggest multiple spawning for both species. Absolute fecundity increased exponentially with

  17. Complete mitogenomes of two Puntius in Taiwan: P. semifasciolatus and P. snyderi (Cypriniformes: Cyprinidae).

    Science.gov (United States)

    Jang-Liaw, Nian-Hong; Chang, Chia-Hao; Tsai, Chi-Li

    2013-06-01

    We sequenced the complete mitochondrial genome of two spotted barbs native to Taiwan: Puntius semifasciolatus and Puntius snyderi. The complete mitochondrial genomes are 16,594 and 16,578 bp in size, respectively. Both of them contain 37 genes coding for 13 proteins, 2 rRNAs, 22 tRNAs, and 1 control region. They share the same gene arrangement pattern that was identical with most vertebrates. Nucleotide sequence divergence (K2P distance) between the two whole mitochondrial genomes was 7.63%. These two spotted barbs show very close relationship based on the comparison of the characters of their mitochondrial genomes.

  18. KHAWIA SAUROGOBII N. SP (CESTODA: CARYOPHYLLIDEA) FROM FRESHWATER FISH SAUROGOBIO SPP. (CYPRINIDAE) IN CHINA

    Czech Academy of Sciences Publication Activity Database

    Xi, B. W.; Oros, Mikuláš; Wang, G. T.; Wu, S. G.; Gao, D.; Nie, P.

    2009-01-01

    Roč. 95, č. 6 (2009), s. 1516-1519 ISSN 0022-3395 R&D Projects: GA ČR GA524/08/0885; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : CESTOIDEA Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.195, year: 2009

  19. FIRST RECORD OF Alburnus atropatenae (BERG, 1925 (CYPRINIDAE IN NAMAK BASIN, CENTRAL IRAN

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    Somaye Khataminejad

    2013-01-01

    Full Text Available Alburnus atropatenae Berg, 1925 is reported for the first time from the River Ghareh-Chay in the Namak basin, central Iran. Previously, this species has only been reported from the Lake Orumiyeh basin, in northwest Iran. In this report twenty-nine specimens were caught on 8 August 2012. Morphometric and meristic characteristics of the specimens were similar to those reported elsewhere for the species.

  20. Squalius namak, a new chub from Lake Namak basin in Iran (Teleostei: Cyprinidae).

    Science.gov (United States)

    Khaefi, Roozbehan; Esmaeili, Hamid Reza; Sayyadzadeh, Golnaz; Geiger, Matthias F; Freyhof, Jörg

    2016-09-19

    Squalius namak, new species, from the endorheic Lake Namak and Kavir basins in Iran, is distinguished from the species of the genus Squalius in the Persian Gulf and the southern Caspian Sea basins by having a wide and thick symphysial knob on the lower jaw, a convex posterior anal-fin margin, a bold, dark-grey or brown, roundish or crescent-shaped blotch at the posterior tip of each flank scale and orange caudal-, anal- and pelvic-fin rays in life. Squalius namak is also characterized by four fixed, diagnostic nucleotide substitutions in the mtDNA COI barcode region.

  1. FIRST RECORD OF Alburnus atropatenae (BERG, 1925) (CYPRINIDAE) IN NAMAK BASIN, CENTRAL IRAN

    OpenAIRE

    Somaye Khataminejad; Hamed Mousavi-Sabet; Masud Sattari; Saber Vatandoust

    2013-01-01

    Alburnus atropatenae Berg, 1925 is reported for the first time from the River Ghareh-Chay in the Namak basin, central Iran. Previously, this species has only been reported from the Lake Orumiyeh basin, in northwest Iran. In this report twenty-nine specimens were caught on 8 August 2012. Morphometric and meristic characteristics of the specimens were similar to those reported elsewhere for the species.

  2. FIRST RECORD OF Alburnusatropatenae (BERG,1925) (CYPRINIDAE) IN NAMAK BASIN, CENTRAL IRAN

    OpenAIRE

    Khataminejad, Somaye; Mousavi-Sabet, Hamed; Sattari, Masud; Vatandoust, Saber

    2013-01-01

    AlburnusatropatenaeBerg, 1925 is reported for the first time from the River Ghareh-Chay in the Namak basin, central Iran. Previously, this species has onlybeen reported from the LakeOrumiyeh basin, in northwest Iran. In this report twenty-nine specimens were caught on 8 August 2012. Morphometric and meristic characteristics of the specimens were similar to those reported elsewhere for the species.

  3. Artificial reproduction of wild and cultured barbel (Barbus barbus, Cyprinidae) under controlled conditions.

    Science.gov (United States)

    Targońska, Katarzyna; Kucharczyk, Dariusz; Zarski, Daniel; Cejko, Beata Irena; Krejszeff, Sławomir; Kupren, Krzysztof; Król, Radosław; Dryl, Katarzyna; Kowalski, Radosław Kajetan; Glogowski, Jan

    2011-09-01

    The aim of this work was to compare the effects of controlled reproduction of cultured and wild common barbel, Barbus barbus (L.). Preparations containing different GnRH analogues and dopamine receptor antagonists (Ovopel, Ovaprim) as well as human chorionic gonadotropin (hCG) (in the case of cultured fish) were applied and their influence on ovulation, spermiation and quality of gametes obtained was determined. No differences in the qualitative or quantitative parameters of semen were found between fish stimulated with different hormonal preparations and those not receiving hormonal stimulation. The high suitability of Ovaprim for ovulation induction in (cultured and wild) barbel was confirmed. The highest synchronisation of ovulation was obtained after the application of Ovopel (18 ± 3 h), but the best results of controlled reproduction (expressed as the percentage of ovulations and survival of embryos) were obtained by applying Ovaprim (83.2 ± 4.1). A significantly higher percentage of ovulation was obtained in cultured fish (80-90%) than in wild fish (< 25%).

  4. Population consequences of behaviour in the European bitterling (Rhodeus sericeus Cyprinidae)

    Czech Academy of Sciences Publication Activity Database

    Smith, C.; Reichard, Martin; Douglas, A.; Jurajda, Pavel

    2006-01-01

    Roč. 15, č. 2 (2006), s. 139-145 ISSN 0906-6691 Institutional research plan: CEZ:AV0Z60930519 Keywords : male-male competition * oviposition choice * population dynamics Subject RIV: EG - Zoology Impact factor: 1.479, year: 2006

  5. A contribution to the data on tench (Tinca tinca L., Cyprinidae, pisces distribution in Serbia

    Directory of Open Access Journals (Sweden)

    Marković Goran S.

    2010-01-01

    Full Text Available The studies on the ichthyofauna of Serbia that have been conducted so far indicate the sporadic tench (Tinca tinca L. presence in typical habitats. The hydroenvironmental conditions necessary for the survival of this fish species are highly variable and, generally, are more favourable in the northern part of the country (the Vojvodina Province. Although the tench presence has been registered at over 30 sites, there is a tendency of a decrease in population abundance. This is due to the reduction of the surface area of the macrophyte-covered flood zones induced by the damming and regulation of rivers, high water level fluctuations, and water amount reduction in the majority of watercourses, a massive increase in the number of allochthonous phytofagous species, reservoir sediment deposition, water quality deterioration and other factors. The tench conservation status in Serbia is officially designated as Low Risk - Least Concern. It may be transferred to a higher category if the tendency towards deterioration of survival conditions for the species should continue.

  6. Sequencing, description and phylogenetic analysis of the mitochondrial genome of Sarcocheilichthys sinensis sinensis (Cypriniformes: Cyprinidae).

    Science.gov (United States)

    Li, Chen; He, Liping; Chen, Chong; Cai, Lingchao; Chen, Pingping; Yang, Shoubao

    2016-01-01

    Sarcocheilichthys sinensis sinensis (Bleeker, 1871), is a small benthopelagic freshwater species with high nutritional and ornamental value. In this study, the complete mitochondrial genome of S. sinensis sinensis was determined; the phylogenetic analysis with another individual and closely related species of Sarcocheilichthys fishes was carried out. The complete mitogenome of S. sinensis sinensis was 16683 bp in length, consist of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 2 non-coding regions: (D-loop and OL). It indicated that D-loop, ND2, and CytB may be appropriate molecular markers for studying population genetics and conservation biology of Sarcocheilichthys fishes.

  7. Tempo and mode of recurrent polyploidization in the Carassius auratus species complex (Cypriniformes, Cyprinidae).

    Science.gov (United States)

    Luo, J; Gao, Y; Ma, W; Bi, X-y; Wang, S-y; Wang, J; Wang, Y-q; Chai, J; Du, R; Wu, S-f; Meyer, A; Zan, R-g; Xiao, H; Murphy, R W; Zhang, Y-p

    2014-04-01

    Polyploidization is an evolutionarily rare but important mechanism in both plants and animals because it increases genetic diversity. Goldfish of the Carassius auratus species complex can be tetraploids, hexaploids and octaploids. Polyploidization events have occurred repeatedly in goldfish, yet the extent of this phenomenon and its phyletic history are poorly understood. We explore the origin, tempo and frequency of polyploidization in Chinese and Japanese goldfish using both mitochondrial (mtDNA) and nuclear DNA sequences from up to 1202 individuals including the outgroup taxon, Cyprinus carpio. Analyses of de novo nuclear gene data resolve two clusters of alleles and the pattern supports the prior hypothesis of an ancient allotetraploidization for Carassius. Alleles shared by tetraploid and hexaploid individuals indicate recent autoploidizations within the C. auratus complex. Sympatric tetraploids and hexaploids share mtDNA haplotypes and these frequently occur independently within six well-supported lineages and sublineages on a small spatial scale. Gene flow estimates (Fst values) indicate that hexaploids differ only slightly from sympatric tetraploids, if at all. In contrast, allopatric populations of tetraploids and hexaploids differ from one another to a far greater extent. Gene flow between sampled localities appears to be limited. Coalescence-based time estimations for hexaploids reveal that the oldest lineage within any sampled locality is around one million years old, which is very young. Sympatric, recurrent autoploidization occurs in all sampled populations of the C. auratus complex. Goldfish experience polyploidization events more frequently than any other vertebrate.

  8. Spawning seasons of Rasbora tawarensis (Pisces: Cyprinidae in Lake Laut Tawar, Aceh Province, Indonesia

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    Musman Musri

    2010-05-01

    Full Text Available Abstract Background Rasbora tawarensis is an endemic freshwater fish in Lake Laut Tawar, Aceh Province, Indonesia. Unfortunately, its status is regarded as critical endangered with populations decreasing in recent years. To date no information on the spawning activities of the fish are available. Therefore, this study provides a contribution to the knowledge on reproductive biology of R. tawarensis especially on spawning seasons as well as basic information for conservation of the species. Methods Monthly sampling was conducted from April 2008 to March 2009 by using selective gillnets. The gonadosomatic index, size composition and sex ratio were assessed. The gonadal development was evaluated based on macroscopic and microscopic examinations of the gonads. Results The gonadosomatic index (GSI varied between 6.65 to 18.16 in female and 4.94 to 8.56 for male. GSI of the female R. tawarensis was higher in March, September and December indicating the onset of reproductive seasons, the GSI and oocyte size being directly correlated with gonadal development stages. Although, a greater proportion of mature male than female was detected during the study, the sex ratio showed that the overall number of female was higher than male. The ovaries had multiple oocyte size classes at every stage of gonadal development, thus R. tawarensis can be classified as a group synchronous spawner or a fractional multiple spawner. Conclusion The spawning seasons of R. tawarensis were three times a year and September being the peak of the reproductive season and the female was the predominant sex. This species is classified as a group synchronous spawner.

  9. Molecular phylogeography and evolutionary history of Poropuntius huangchuchieni (Cyprinidae in Southwest China.

    Directory of Open Access Journals (Sweden)

    Xiaoyun Wu

    Full Text Available The evolution of the Yunnan Plateau's drainages network during the Pleistocene was dominated by the intense uplifts of the Qinghai-Tibetan Plateau. In the present study, we investigated the association between the evolutionary histories of three main drainage systems and the geographic patterns of genetic differentiation of Poropuntius huangchuchieni.We sequenced the complete sequences of mitochondrial control region for 304 specimens and the sequences of Cytochrome b gene for 15 specimens of the species P. huangchuchieni and 5 specimens of Poropuntius opisthoptera. Phylogenetic analysis identified five major lineages, of which lineages MK-A and MK-B constrained to the Mekong River System, lineages RL and LX to the Red River System, and lineage SW to the Salween River System. The genetic distance and network analysis detected significant divergences among these lineages. Mismatch distribution analysis implied that the population of P. huangchuchieni underwent demographic stability and the lineage MK-B, sublineages MK-A1 and LX-1 underwent a recent population expansion. The divergence of the 5 major lineages was dated back to 0.73-1.57 MYA.Our results suggest that P. opisthoptera was a paraphyletic group of P. huangchuchieni. The phylogenetic pattern of P. huangchuchieni was mostly associated with the drainage's structures and the geomorphological history of the Southwest Yunnan Plateau. Also the differentiation of the major lineages among the three drainages systems coincides with the Kunlun-Yellow River Movement (1.10-0.60 MYA. The genetic differentiation within river basins and recent demographical expansions that occurred in some lineages and sublineages are consistent with the palaeoclimatic oscillations during the Pleistocene. Additionally, our results also suggest that the populations of P. huangchuchieni had keep long term large effective population sizes and demographic stability in the recent evolutionary history, which may be responsible for the high genetic diversity and incomplete lineages sorting of Poropuntius huangchuchieni.

  10. Two new species of shovel-jaw carp Onychostoma (Teleostei: Cyprinidae) from southern Vietnam.

    Science.gov (United States)

    Hoang, Huy Duc; Pham, Hung Manh; Tran, Ngan Trong

    2015-05-22

    Two new species of large shovel-jaw carps in the genus Onychostoma are described from the upper Krong No and middle Dong Nai drainages of the Langbiang Plateau in southern Vietnam. These new species are known from streams in montane mixed pine and evergreen forests between 140 and 1112 m. Their populations are isolated in the headwaters of the upper Sre Pok River of the Mekong basin and in the middle of the Dong Nai basin. Both species are differentiated from their congeners by a combination of the following characters: transverse mouth opening width greater than head width, 14-17 predorsal scales, caudal-peduncle length 3.9-4.2 times in SL, no barbels in adults and juveniles, a strong serrated last simple ray of the dorsal fin, and small eye diameter (20.3-21.5% HL). Onychostoma krongnoensis sp. nov. is differentiated from Onychostoma dongnaiensis sp. nov. by body depth (4.0 vs. 3.2 times in SL), predorsal scale number (14-17 vs. 14-15), dorsal-fin length (4.5 vs. 4.2 times in SL), caudal-peduncle length (3.9 vs. 4.2 times in SL), colour in life (dark vs. bright), and by mitochondrial DNA (0.2% sequence divergence). Molecular evidence indicates that both species are members of Onychostoma and are distinct from all congeners sampled (uncorrected sequence divergences at the 16S rRNA gene of >2.0% for all Onychostoma for which homologous 16S rRNA sequences are available).

  11. Microsatellite Development for an Endangered Bream Megalobrama pellegrini (Teleostei, Cyprinidae Using 454 Sequencing

    Directory of Open Access Journals (Sweden)

    Zuogang Peng

    2012-03-01

    Full Text Available Megalobrama pellegrini is an endemic fish species found in the upper Yangtze River basin in China. This species has become endangered due to the construction of the Three Gorges Dam and overfishing. However, the available genetic data for this species is limited. Here, we developed 26 polymorphic microsatellite markers from the M. pellegrini genome using next-generation sequencing techniques. A total of 257,497 raw reads were obtained from a quarter-plate run on 454 GS-FLX titanium platforms and 49,811 unique sequences were generated with an average length of 404 bp; 24,522 (49.2% sequences contained microsatellite repeats. Of the 53 loci screened, 33 were amplified successfully and 26 were polymorphic. The genetic diversity in M. pellegrini was moderate, with an average of 3.08 alleles per locus, and the mean observed and expected heterozygosity were 0.47 and 0.51, respectively. In addition, we tested cross-species amplification for all 33 loci in four additional breams: M. amblycephala, M. skolkovii, M. terminalis, and Sinibrama wui. The cross-species amplification showed a significant high level of transferability (79%–97%, which might be due to their dramatically close genetic relationships. The polymorphic microsatellites developed in the current study will not only contribute to further conservation genetic studies and parentage analyses of this endangered species, but also facilitate future work on the other closely related species.

  12. Age and growth of Labeo gonius (Cyprinidae) in Kali river, Uttar Pradesh, India

    Digital Repository Service at National Institute of Oceanography (India)

    Chatterji, A.

    stream_size 4 stream_content_type text/plain stream_name Asian_Fish_Sci_5_257.pdf.txt stream_source_info Asian_Fish_Sci_5_257.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 ...

  13. The influence of low-level radiation on nucleolus activity of cyprinidae embryos

    International Nuclear Information System (INIS)

    Arkhipchuk, V.V.

    1990-01-01

    The number and sizes of nucleoli in embryos of Cyprinus carpio and Carassius auratus gibelio exposed to low-level radiation at a dose-rate of 10 μ Gy/h and down were larger than those on non-irradiated controls

  14. Threatened Fishes of the World: Telestes Croaticus (Steindachner, 1866 (Cypriniformes: Cyprinidae

    Directory of Open Access Journals (Sweden)

    Jelić Dušan

    2017-03-01

    Full Text Available Telestes croaticus is a freshwater fish endemic in Croatia. It is a stygophile species, living above ground but occasionally retreating into subterranean waters. This species is found in the southwest region of Croatia, in the Lika-Jadova and Ričica drainages, in cold and clear waters in lowland habitats with little current, and in springs and associated wetlands. It is protected under Croatian law and was listed by the IUCN Red List as Endangered (EN. It has an extremely limited distribution which makes it vulnerable to different kind of traits such as habitat destruction, water extraction, dam construction, agricultural water pollution and especially the introduction of non-indigenous fish species. Suggested conservation actions for this species are: bans on watercourse regulation, reduction of pollution and eradication of alien fish species. Due to its fast decline in the last 10 years, it is our proposition that T. croaticus should be upgraded to a Critically Endangered species under criterion A2ace A4ace.

  15. Karyotype and chromosome banding of endangered crucian carp, carassius carassius (Linnaeus, 1758) (Teleostei, Cyprinidae)

    Czech Academy of Sciences Publication Activity Database

    Knytl, M.; Kalous, L.; Ráb, Petr

    2013-01-01

    Roč. 7, č. 3 (2013), s. 205-215 ISSN 1993-0771 R&D Projects: GA ČR(CZ) GPP506/11/P596 Institutional support: RVO:67985904 Keywords : fish cytogenetics * paleotetraploid * heterochromatin * metaphase chromosomes Subject RIV: EG - Zoology Impact factor: 1.211, year: 2013

  16. BIOCHEMICAL AND HISTOLOGICAL EFFECTS OF DELTAMETHRIN EXPOSURE ON THE GILLS OF CARASSIUS AURATUS GIBELIO (Pisces Cyprinidae

    Directory of Open Access Journals (Sweden)

    DIANA COSTIN

    2007-05-01

    Full Text Available This study investigated the alterations in the activities of several antioxidant enzymes inthe gills of the freshwater fish Carassius auratus gibelio exposed to deltamethrin. To getthis goal, groups of 10 individuals were exposed for one, two, three, seven and fourteendays to sublethal concentration of deltamethrin (2 µg/L. Another group was used ascontrol. The activities of catalase, gluthatione peroxidase and gluthatione reductasewere significantly decreased, while the glutathione-S-transferase was up-regulated. Allfish, exposed to 2 µg/L deltamethrin revealed gills morphological alterations after 48h ofexposure which were accentuated after 14 days. In the gills hyperemia, fusion ofsecondary lamellae, epithelial layer rupture and chloride cells proliferation wereobserved. These results suggest that an immediate adaptive response to the oxidativestress appeared, demonstrating alterations in the antoxidant defense mechanism in thegills of deltamethrin intoxicated fish.

  17. Reproductive biology of Garra regressus and Garra tana (Cypriniformes: Cyprinidae from Lake Tana, Ethiopia

    Directory of Open Access Journals (Sweden)

    Akewake Geremew

    2015-05-01

    Full Text Available The reproductive biology of Garra regressus and Garra tana was investigated by collecting monthly samples (January to December 2006 from the southern Gulf of Lake Tana, where these species are endemic.  Garra regressus has an extended breeding time from April to October while G. tana breeds throughout the year with a peak from March to July.  The mean size at maturity in both the species was not significantly different between the sexes, but G. tana had a significantly lower mean size at maturity than G. regressus in female specimens.  Absolute fecundity estimates for G. regressus ranged from 580.8–1800, while those for G. tana ranged from 538.9–2968.  Egg size frequency distribution revealed that G. regressus is a multiple spawner, while G. tana is a single spawner.  The sex ratio in the total catch of G. regressus was found to be skewed in favour of females (Chi-square, P< 0.05, while those for G. tana was not significantly different from 1:1. The mean size at maturity was not significantly different between the sexes for G. tana. 

  18. Comparative cytogenetics and chromosomal characteristics of ribosomal DNA in the fish genus Vimba (Cyprinidae)

    Czech Academy of Sciences Publication Activity Database

    Rábová, Marie; Ráb, Petr; Ozouf-Costaz, C.; Ene, C.; Wanzebock, J.

    2003-01-01

    Roč. 118, č. 1 (2003), s. 83-91 ISSN 0016-6707 R&D Projects: GA AV ČR IAA6045704 Institutional research plan: CEZ:AV0Z5045916 Keywords : chromosome banding * cytotaxonomy of Vimba * FISH Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.057, year: 2003

  19. Ecology and potential for fishery of the small barbs (Cyprinidae, Teleostei) of Lake Tana, Ethiopia

    OpenAIRE

    Dejen, E.

    2003-01-01

    Lake Tana is by far the largest lake of Ethiopia and source of the Blue Nile. By feeding on zooplankton, small barbs (< 10 cm) occupy a central position in Lake Tana's ecosystem. Catching them could release pressure on the overexploited, unique species flock of large barbs (up to 100 cm). Aiming at small barbs, we need first to assess its possible impact on the food web and productivity. To advise on sustainable management, the biology and ecology of the small Barbus species in Lake Tana w...

  20. Ecology and potential for fishery of the small barbs (Cyprinidae, Teleostei) of Lake Tana, Ethiopia

    NARCIS (Netherlands)

    Dejen, E.

    2003-01-01

    Lake Tana is by far the largest lake of Ethiopia and source of the Blue Nile. By feeding on zooplankton, small barbs (< 10 cm) occupy a central position in Lake Tana's ecosystem. Catching them could release pressure on the overexploited, unique species flock of large barbs (up to 100 cm).

  1. Molecular Phylogeny and Zoogeography of the Capoeta damascina Species Complex (Pisces: Teleostei: Cyprinidae.

    Directory of Open Access Journals (Sweden)

    Nisreen Alwan

    Full Text Available Capoeta damascina was earlier considered by many authors as one of the most common freshwater fish species found throughout the Levant, Mesopotamia, Turkey, and Iran. However, owing to a high variation in morphological characters among and within its various populations, 17 nominal species were described, several of which were regarded as valid by subsequent revising authors. Capoeta damascina proved to be a complex of closely related species, which had been poorly studied. The current study aims at defining C. damascina and the C. damascina species complex. It investigates phylogenetic relationships among the various members of the C. damascina complex, based on mitochondrial and nuclear DNA sequences. Phylogenetic relationships were projected against paleogeographical events to interpret the geographic distribution of the taxa under consideration in relation to the area's geological history. Samples were obtained from throughout the geographic range and were subjected to genetic analyses, using two molecular markers targeting the mitochondrial cytochrome oxidase I (n = 103 and the two adjacent divergence regions (D1-D2 of the nuclear 28S rRNA genes (n = 65. Six closely related species were recognized within the C. damascina complex, constituting two main lineages: A western lineage represented by C. caelestis, C. damascina, and C. umbla and an eastern lineage represented by C. buhsei, C. coadi, and C. saadii. The results indicate that speciation of these taxa is rather a recent event. Dispersal occurred during the Pleistocene, resulting in present-day distribution patterns. A coherent picture of the phylogenetic relationships and evolutionary history of the C. damascina species complex is drawn, explaining the current patterns of distribution as a result of paleogeographic events and ecological adaptations.

  2. Molecular Phylogeny and Zoogeography of the Capoeta damascina Species Complex (Pisces: Teleostei: Cyprinidae).

    Science.gov (United States)

    Alwan, Nisreen; Esmaeili, Hamid-Reza; Krupp, Friedhelm

    2016-01-01

    Capoeta damascina was earlier considered by many authors as one of the most common freshwater fish species found throughout the Levant, Mesopotamia, Turkey, and Iran. However, owing to a high variation in morphological characters among and within its various populations, 17 nominal species were described, several of which were regarded as valid by subsequent revising authors. Capoeta damascina proved to be a complex of closely related species, which had been poorly studied. The current study aims at defining C. damascina and the C. damascina species complex. It investigates phylogenetic relationships among the various members of the C. damascina complex, based on mitochondrial and nuclear DNA sequences. Phylogenetic relationships were projected against paleogeographical events to interpret the geographic distribution of the taxa under consideration in relation to the area's geological history. Samples were obtained from throughout the geographic range and were subjected to genetic analyses, using two molecular markers targeting the mitochondrial cytochrome oxidase I (n = 103) and the two adjacent divergence regions (D1-D2) of the nuclear 28S rRNA genes (n = 65). Six closely related species were recognized within the C. damascina complex, constituting two main lineages: A western lineage represented by C. caelestis, C. damascina, and C. umbla and an eastern lineage represented by C. buhsei, C. coadi, and C. saadii. The results indicate that speciation of these taxa is rather a recent event. Dispersal occurred during the Pleistocene, resulting in present-day distribution patterns. A coherent picture of the phylogenetic relationships and evolutionary history of the C. damascina species complex is drawn, explaining the current patterns of distribution as a result of paleogeographic events and ecological adaptations.

  3. Long-term persistence, density dependence and effects of climate change on rosyside dace (Cyprinidae)

    Science.gov (United States)

    Gary D. Grossman; Gary Sundin; Robert E. Ratajczak

    2016-01-01

    SummaryWe used long-term population data for rosyside dace (Clinostomus funduloides), a numerically dominant member of a stochastically organised fish assemblage, to evaluate the relative importance of density-dependent and density-independent processes to population...

  4. Insights on the Reproduction and Embryonic Development of Garra rufa (Cyprinidae

    Directory of Open Access Journals (Sweden)

    Mónica R S Gomes

    2015-11-01

    In a total of 28 postures, only 17 resulted in newly born fish. This was mostly due to fungal development around the eggs that caused the embryos to degenerate. There was a hatching success of 60%. The eggs showed no adhesive properties, being deposited on the bottom. The most prominent structures of the embryos were noticeable after: 3h - tail bud; 6h - optic primordium; 10h - heart beating; 15h - pectoral fins buds. They hatched between 24 to 48h and the larvae consumed the yolk sac in 48h.

  5. Estimation of the ideal dosage and feeding frequency for Garra rufa (Cyprinidae

    Directory of Open Access Journals (Sweden)

    Mafalda M. R. S. Catarino

    2015-11-01

    Full Text Available Garra rufa are widely used in skin treatments at beauty and wellness institutes. This freshwater species is native from Eurasia and has a large distribution area, including river basins in Turkey and Iran. It has a high commercial value and arouse interest among fish farmers. Even so, G. rufa methods of production are still unknown. In fact, aspects that sustain a rapid and healthy growth of these fish need to be investigated, like an adequate daily dose and feeding frequency. This work aimed to establish: 1 the optimal feed dose per day, correspondent to the amount necessary for obtaining satiation, with a commercial granulated food; and 2 the optimal frequency of feeding for intensive production of G. rufa in Aquaculture. A total of 60 fishes underwent a 3 weeks acclimation period in laboratory, distributed by 6 aquaria, containing 10 fishes with similar body weight and length. The animals were initially fed with a dose correspondent to 10% of their body weight. This dose was gradually increased until complete satiation of the fishes. An optimal dose of 0.04 g fish-1 day-1 was found. Afterwards, the same fishes went through a 1 month experimental trial to evaluate the ideal feeding frequency. Fish growth was compared between feeding 0.2 g twice per day and 0.1333 g three times per day. Variations in size (total and fork length, plus body weight were evaluated at the end of the trial. One-Way ANOVA procedures revealed that the variations in weight and in fork length were similar between treatments, but the variation in total length was significantly higher with 3 daily feeding moments (p<0.05. In conclusion, the present results showed that the optimal dose that ensures satiation for G. rufa is 0.04 g fish-1 day-1 and that 3 daily feeding moments promotes higher growth rates.

  6. The reserpine effects on the gonadotrophic cells of the male common carp Cyprinus carpio (Osteichtyes: Cyprinidae

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    Lilian González-Segura

    2004-03-01

    Full Text Available The secretion of gonadotropins (GtH in goldfish and carp, is stimulated by GtH-releasing hormone (GnRH and is inhibited by dopamine. Studies with antidopaminergics have demonstrated to be effective in order to stimulate the spermiation and the ovulation in different species of teleosts. The reserpine, a drug that deplets the dopamine, has shown to stimulate the spermiation in the common carp. We report here, the effects of reserpine on the number and volume of gonadotrophic cells of the common carp. Eight injections of reserpine alone, at doses of 0.5, 1.0 or 1.5 mg/ml/kg of body weight and at intervals of 48 hours, caused an increase in the number and volume of gonadotrophic cells. The dose 0.5 mg/ml/kg, presented an increase in the number and volume of gonadotrophic cells of 382% and 123%, respectively, above the control group. The dose 1.0 mg/ml/kg, showed an enhanced number and volume of gonadotrophic cells of 704% and 152%, respectively. With the dose 1.5 mg/ml/kg increase in number (171% and volume (106% of gonadotrophic cells was lower. The gonads of the experimental groups had an abundance of advanced states of spermatogenesis. Our results show that eight intraperitoneal injections of reserpine were responsible for an increase in gonadodrophic cell, number and volumeLa secreción de gonadotropinas (GtHs en pez dorado y carpas, es estimulada por la hormona liberadora de gonadotropinas (GnRH e inhibida por la dopamina. Trabajos previos con antidopaminérgicos demostraron ser efectivos para estimular la espermiación y ovulación, en varias especies de teleósteos. La reserpina, una sustancia que desaparece catecolaminas, se ha probado con éxito para estimular la espermiación en la carpa común. En este trabajo, reportamos los efectos de la reserpina en el número y volúmen de células gonadotropas en la carpa común. Se aplicaron ocho inyecciones de reserpina a dosis de 0.5, 1.0, y 1.5 mg/ml/kg de peso corporal, a intervalos de 48 horas entre cada inyección. Con la dosis de 0.5 mg/ml/kg, se encontró un aumento en el número de gonadotrofas de 382% y de 123% en el volumen, en comparación con los controles. La dosis de 1.0 mg/ml/kg presentó un aumento de 704% en el número de células y de 152% en el volumen celular. Con 1.5 mg/ml/kg se causó que las gonadotrofas aumentarán 171% en número y 106% en volumen. En general, las gónadas de los grupos experimentales presentaron estadios más avanzados de espermatogénesis. Nuestros resultados demuestran que ocho aplicaciones de reserpina provocan el incremento, tanto del número como del volumen, de células gonadotropas

  7. Introgressive hybridization as a promoter of genome reshuffling in natural homoploid fish hybrids (Cyprinidae, Leuciscinae)

    Czech Academy of Sciences Publication Activity Database

    Pereira, C. S.; Aboim, M. A.; Ráb, Petr; Collares-Pereira, M. J.

    2014-01-01

    Roč. 112, č. 3 (2014), s. 343-350 ISSN 0018-067X Institutional support: RVO:67985904 Keywords : comparative genome hybridization * hybrid zones * introgression Subject RIV: EG - Zoology Impact factor: 3.805, year: 2014

  8. Excess post-hypoxic oxygen consumption is independent from lactate accumulation in two cyprinid fishes

    DEFF Research Database (Denmark)

    Genz, J.; Jyde, M.B.; Svendsen, Jon Christian

    2013-01-01

    the increase in oxygen consumption in fish required following strenuous exercise or low environmental oxygen availability has been frequently considered, the primary contributing mechanism remains unknown. This study utilized the close relationship but strongly divergent physiology between C. carpio and C...

  9. Early life-history predator-prey reversal in two cyprinid fishes

    Czech Academy of Sciences Publication Activity Database

    Šmejkal, Marek; Baran, Roman; Blabolil, Petr; Vejřík, Lukáš; Prchalová, Marie; Bartoň, Daniel; Mrkvička, Tomáš; Kubečka, Jan

    2017-01-01

    Roč. 7, JUL (2017), č. článku 6924. ISSN 2045-2322 R&D Projects: GA MŠk(CZ) 7F14316; GA ČR GPP505/12/P647 Grant - others:AV ČR(CZ) StrategieAV21/9 Program:StrategieAV Institutional support: RVO:60077344 Keywords : predator-prey relationship * fish spawning * bleak * asp Subject RIV: EH - Ecology, Behaviour OBOR OECD: Ecology Impact factor: 4.259, year: 2016

  10. Condition-dependent individual decision-making determines cyprinid partial migration

    DEFF Research Database (Denmark)

    Brodersen, J.; Nilsson, P.A.; Hansson, L.A.

    2008-01-01

    with decreased access to food. Furthermore, fewer unfed migrants returned to the lake, indicating higher overwinter mortality. Our results suggest that individual fish trade off safety from predation and access to food differently depending on their body condition, which results in a condition-dependent partial...... migration. Hence, our main conclusion is that individual decision-making is based on assessment of own condition which offers a mechanistic explanation to partial migration. Moreover, this may be of high importance for understanding population responses to environmental variation as well as ecosystem...

  11. Seasonal dynamics and age structure of drifting cyprinid fishes: an interspecific comparison

    Czech Academy of Sciences Publication Activity Database

    Reichard, Martin; Jurajda, Pavel

    2007-01-01

    Roč. 16, č. 4 (2007), s. 482-492 ISSN 0906-6691 R&D Projects: GA MŠk LC06073 Institutional research plan: CEZ:AV0Z60930519 Keywords : dispersal * downstream drift * early life history * migration * young-of-the-year-fish Subject RIV: EH - Ecology, Behaviour Impact factor: 1.206, year: 2007

  12. Who is who: An anomalous predator-prey role exchange between cyprinids and perch.

    Czech Academy of Sciences Publication Activity Database

    Vejřík, Lukáš; Matějíčková, Ivana; Seďa, Jaromír; Blabolil, Petr; Jůza, Tomáš; Vašek, Mojmír; Ricard, Daniel; Matěna, Josef; Frouzová, Jaroslava; Kubečka, Jan; Říha, Milan; Čech, Martin

    2016-01-01

    Roč. 11, č. 6 (2016), č. článku e0156430. E-ISSN 1932-6203 R&D Projects: GA MŠk(CZ) EE2.3.20.0204; GA MŠk(CZ) 7F14316; GA ČR GP206/09/P266; GA ČR GA15-24309S; GA MŠk(CZ) EE2.3.30.0032 Institutional support: RVO:60077344 Keywords : Abramis brama * Cyprinus carpio * piscivory * YOY perch * zooplankton Subject RIV: DA - Hydrology ; Limnology Impact factor: 2.806, year: 2016

  13. A foraging cost of migration for a partially migratory cyprinid fish

    DEFF Research Database (Denmark)

    Chapman, Ben B; Eriksen, Anders; Baktoft, Henrik

    2013-01-01

    Rutilus rutilus, which migrates from shallow lakes to streams during winter. By sampling fish from stream and lake habitats in the autumn and spring and measuring their stomach fullness and diet composition, we tested if migrating roach pay a cost of reduced foraging when migrating. Resident fish had...... fuller stomachs containing more high quality prey items than migrant fish. Hence, we document a feeding cost to migration in roach, which adds additional support for the validity of the p/g model of migration in freshwater systems....

  14. Pool-Type Fishways: Two Different Morpho-Ecological Cyprinid Species Facing Plunging and Streaming Flows

    Science.gov (United States)

    Branco, Paulo; Santos, José M.; Katopodis, Christos; Pinheiro, António; Ferreira, Maria T.

    2013-01-01

    Fish are particularly sensitive to connectivity loss as their ability to reach spawning grounds is seriously affected. The most common way to circumvent a barrier to longitudinal connectivity, and to mitigate its impacts, is to implement a fish passage device. However, these structures are often non-effective for species with different morphological and ecological characteristics so there is a need to determine optimum dimensioning values and hydraulic parameters. The aim of this work is to study the behaviour and performance of two species with different ecological characteristics (Iberian barbel Luciobarbus bocagei–bottom oriented, and Iberian chub Squalius pyrenaicus–water column) in a full-scale experimental pool-type fishway that offers two different flow regimes–plunging and streaming. Results showed that both species passed through the surface notch more readily during streaming flow than during plunging flow. The surface oriented species used the surface notch more readily in streaming flow, and both species were more successful in moving upstream in streaming flow than in plunging flow. Streaming flow enhances upstream movement of both species, and seems the most suitable for fishways in river systems where a wide range of fish morpho-ecological traits are found. PMID:23741465

  15. Several Growth Characteristics of an Invasive Cyprinid Fish (Carassius gibelio Bloch, 1782

    Directory of Open Access Journals (Sweden)

    Sait BULUT

    2013-05-01

    Full Text Available Age composition, length-weight relationships, growth, and condition factors of the gibel carp (Carassius gibelio Bloch, 1782 were determined using specimens collected from Seyitler Reservoir between July 2005 to June 2006. A total of 149 gibel carp were observed and examined. The age composition of the samples ranged between I and VII years of age. It has been determined than 82.55% of the obtained samples are comprised of females, 16.11% is comprised of males and 1.34% is comprised of immature. The population is dominated by females able to reproduce gynogenetically. The mean fork lengths and mean weights of the population were 14.8-32.5 cm and 43.1-807.3 g respectively. The length-weight relation were calculated as W = 0.0696 L2.132, r=0.838 for females, for males W = 0.2942 L2.6417 r=0.784 and W = 0.0274 L2.9382, r=0.813 for all samples. The mean Fulton Condition Factor was calculated as 2.342 for females, 2.064 for males and 2.276 for all samples. Age-length and age-weight relations were determined according to von Bertalanffy growth equation formula. Growth parameters of the population were Lt = 48.09 [1-e-0.093(t+0.29], and Wt=2323.62 [1-e-0.093(t+0.29]2.9382. The growth performance index value (Ø´ was computed as 5.37 for all specimens.

  16. Diet of the cyprinid fish Barbus aeneus (Burchell) in the P .K. Ie Roux ...

    African Journals Online (AJOL)

    structure of the ecosystem, and fail to take into account ... by restricting photosynthesis but also, by restricting solar ... radiation and evaporation, and contributed to the generally .... Autumn (March - May) - negative heat balance, stratifIca-.

  17. Relação preliminar das espécies de peixes (Pisces, Elasmobranchii, Actinopterygii ameaçadas no Brasil Preliminary list of endangered fish species (Pisces, Elasmobranchii , Actinopterygii in Brazil

    Directory of Open Access Journals (Sweden)

    Ricardo S Rosa

    1996-01-01

    Full Text Available The Brazilian fish fauna is still poorly known with respect to its diversity and conservation status, particularly of freshwater species. Human activities and population growth are rapidly impacting this fauna to an unchecked extent, so that many fish species are presently threatened. Also, some areas have been so critically altered that their fish fauna can no longer be properly inventoried. There is an urgent need to evaluate the conservation status of Brazilian fish species, since none are presently listed as endangered by federal legislation. Based on a literature search and a survey directed to the Neotropical ichthyologists, we present an unofficial list including a total of 78 threatened fish species (12 elasmobranchs and 66 actinopterygians. Of these, sixteen actinopterygian species are listed as endangered, three elasmobranchs and nine actinopterygians as vulnerable, and seven actinopterygians as rare; 40 species either have indeterminate status or are data deficient to yield an adequate assessment, and three species are listed as extinct or possibly extinct.

  18. Aphanius arakensis, a new species of tooth-carp (Actinopterygii, Cyprinodontidae from the endorheic Namak Lake basin in Iran

    Directory of Open Access Journals (Sweden)

    Azad Teimori

    2012-08-01

    Full Text Available A new species of tooth-carp, Aphanius arakensis sp. n., is described from the Namak Lake basin in Iran. The new species is distinguished by the congeners distributed in Iran by the following combination of characters: 10–12 anal fin rays, 28–32 lateral line scales, 10–13 caudal peduncle scales, 8–10 gill rakers, 12–19, commonly 15–16, clearly defined flank bars in males, a more prominent pigmentation along the flank added by relatively big blotches in the middle and posterior flank segments in females, a short but high antirostrum of the otolith that has a wide excisura, and a ventral rim with some small, drop-like processes, and 19 molecular apomorphies (17 transitions, two transversions in the cytochrome b gene. It was suggested based on the phylogenetic analysis that the new species is sister to A. sophiae from the Kor River and that A. farsicus from the Maharlu Lake basin is sister to A. arakensis plus A. sophiae. A noticeable feature of the Aphanius diversity in Iran is the conservatism of the external morphology as well as morphometric and meristic characters, while distinctive differences are present in genetic characters, otolith morphology, and male color pattern. Transformation of the latter was probably driven by sexual selection.

  19. Aphanius arakensis, a new species of tooth-carp (Actinopterygii, Cyprinodontidae) from the endorheic Namak Lake basin in Iran.

    Science.gov (United States)

    Teimori, Azad; Esmaeili, Hamid Reza; Gholami, Zeinab; Zarei, Neda; Reichenbacher, Bettina

    2012-01-01

    A new species of tooth-carp, Aphanius arakensissp. n., is described from the Namak Lake basin in Iran. The new species is distinguished by the congeners distributed in Iran by the following combination of characters: 10-12 anal fin rays, 28-32 lateral line scales, 10-13 caudal peduncle scales, 8-10 gill rakers, 12-19, commonly 15-16, clearly defined flank bars in males, a more prominent pigmentation along the flank added by relatively big blotches in the middle and posterior flank segments in females, a short but high antirostrum of the otolith that has a wide excisura, and a ventral rim with some small, drop-like processes, and 19 molecular apomorphies (17 transitions, two transversions) in the cytochrome b gene. It was suggested based on the phylogenetic analysis that the new species is sister to Aphanius sophiae from the Kor River and that Aphanius farsicus from the Maharlu Lake basin is sister to Aphanius arakensis plus Aphanius sophiae. A noticeable feature of the Aphanius diversity in Iran is the conservatism of the external morphology as well as morphometric and meristic characters, while distinctive differences are present in genetic characters, otolith morphology, and male color pattern. Transformation of the latter was probably driven by sexual selection.

  20. Aphanius arakensis, a new species of tooth-carp (Actinopterygii, Cyprinodontidae) from the endorheic Namak Lake basin in Iran

    OpenAIRE

    Teimori,Azad; Esmaeili,Hamid; Gholami,Zeinab; Zarei,Neda; Reichenbacher,Bettina

    2012-01-01

    A new species of tooth-carp, Aphanius arakensis sp. n., is described from the Namak Lake basin in Iran. The new species is distinguished by the congeners distributed in Iran by the following combination of characters: 10–12 anal fin rays, 28–32 lateral line scales, 10–13 caudal peduncle scales, 8–10 gill rakers, 12–19, commonly 15–16, clearly defined flank bars in males, a more prominent pigmentation along the flank added by relatively big blotches in the m...

  1. Activity, habitat use, feeding behavior, and diet of four sympatric species of Serranidae (Actinopterygii: Perciformes in southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Fernando Zaniolo Gibran

    Full Text Available Diplectrum formosum (the sand perch, Diplectrum radiale (the aquavina, Epinephelus marginatus (the dusky grouper and Mycteroperca acutirostris (the comb grouper are four sympatric serranid of remarkable ecological and commercial importance. This study investigated the feeding of these four species in the São Sebastião Channel, São Paulo State (southeastern Brazil, comparing their diet, habitat utilization and morphological features related to foraging. These four serranids are opportunistic visual predators that use a total of nine different tactics to capture their preys, feed mostly on crustaceans during the day and twilight, and keep stationary and quiescent at night. The ecomorphological study was conducted to test its predictive value to infer lifestyles. It was verified that such analysis should be used carefully and must be complemented by field observations. Although morphologically and behaviorally very similar, D. formosum and D. radiale showed different preferences to the substratum type used by large individuals, which are exclusively sit-and-wait predators. On the other hand, E. marginatus displayed more sedentary habits than M. acutirostris, the most versatile predator from the studied species. The studied species of Diplectrum and E. marginatus can be regarded as benthic serranids, while M. acutirostris could be viewed as a nektobenthic species.

  2. Spatial structure of helminth communities in the golden grey mullet, Liza aurata (Actinopterygii: Mugilidae), from the Western Mediterranean.

    Science.gov (United States)

    Míguez-Lozano, Raúl; Pardo-Carranza, Trinidad V; Blasco-Costa, Isabel; Balbuena, Juan Antonio

    2012-10-01

    Ecological investigations regarding the parasite fauna of grey mullets are scarce. The present study provides a detailed description of the helminth communities of Liza aurata in the Spanish western Mediterranean and analyzes the role of spatial, temporal, and host variables in shaping the infracommunities. In total, 204 fish were collected in 2 localities, situated ca. 290 km apart, in spring and fall of 2004 and 2005. A non-metric multidimensional scaling (NMDS) was used to visualize an ordination of the infracommunities according to their relative similarities in parasite abundances. The relationship between infracommunity composition and explanatory variables (host size, locality, year, and season of harvest) was examined by permutational analysis of variance (PERMANOVA) applied to species abundances. Permutational tests for homogeneity of multivariate dispersion were used to test the null hypothesis of no differences in dispersion among groups formed by the factors whose effects were significant in PERMANOVA. A total of 33,241 helminth parasites, belonging to 18 species, was collected, i.e., 12 species of adult digeneans (23% of the parasite specimens), 3 digeneans as metacercariae (68%), 1 acanthocephalan (2.1%), and 2 monogeneans (6.5%). An important part of this helminth fauna is specialized to grey mullets, with a sizable portion of the component community restricted to the Mediterranean and northeast Atlantic. The NMDS ordination indicated high heterogeneity among infrapopulations. However, most differences at both the component and infracommunity level were related to geographic locality. In fact, the PERMANOVA showed that, among the explanatory variables considered, sampling locality accounted for the largest share of variation. The geographical differences observed may be related to local environmental characteristics or to the limited spatial dispersal of the species forming the component community. The latter was supported by the significant portion of variation explained by a 3-way interaction term. Thus, the spatial structure of our helminth infracommunities seems to be determined by a combination of differences in local environmental conditions and the transmission ability of each species at small local and time scales.

  3. First record of the Alboran dragonet, Protogrammus alboranensis (Actinopterygii: Callionymiformes: Callionymidae), from the Balearic Islands (western Mediterranean)

    OpenAIRE

    Fricke, R. (Ronald); Ordines, F. (Francesc)

    2017-01-01

    The Alboran dragonet, Protogrammus alboranensis Fricke, Ordines, Farias et García-Ruiz, 2016, was originally described based on four specimens from Alboran Island, Spain, south-western Mediterranean, collected in 2014 and 2015. This species was previously considered to be endemic to Alboran Island. A surprising new record of this rare species from the Balearic Islands is reported here Versión del editor

  4. A NEW EARLY SEMIONOTIDAE (SEMIONOTIFORMES, ACTINOPTERYGII FROM THE UPPER LADINIAN OF MONTE SAN GIORGIO AREA SOUTHERN SWITZERLAND AND NORTHERN ITALY

    Directory of Open Access Journals (Sweden)

    ANDREA TINTORI

    2007-11-01

    Full Text Available The early history of the Semionotiformes is still scarcely known as, apart from the Permian Acentrophorus, most of the Early and Middle Triassic taxa traditionally ascribed to this order are often poorly known and/or they actually belong to other groups. Sangiorgioichthys aldae gen. n. sp. n. is described from the Upper Ladinian Kalkschieferzone (Upper Meride Limestone of the Monte San Giorgio area on specimens coming from both Italy and Switzerland. The new genus is ascribed to the family Semionotidae as it shows several of the characters usually considered as diagnostic of the family itself, even if no real synapomorphies have been so far proposed for it. As a Semionotidae, Sangiorgioichthys gen. n. has an almost complete circumorbital series, large ascending process of the premaxilla, rostral and nasals almost tube-like, short maxilla, several suborbital bones posterior and ventral to the infraorbitals, long antero-dorsal process of the suboperculum, sensory canal system very rich in pores, mid dorsal ridge scales with an incipient spine. Peculiar characters of the new genus are dermal skull bones completely covered by ganoine, a very large ventral infraorbital almost attaining the anterior edge of the preoperculum, two ‘ventral’ suborbitals, very elongate anterior supraorbital bone,  dentition made of a number of tiny pencil-like teeth, anterior and posterior lateral line scales differentiated. 

  5. Reproduction of Baltic cod, Gadus morhua (Actinopterygii: Gadiformes: Gadidae), in the Gotland Basin: Causes of annual variability

    DEFF Research Database (Denmark)

    Plikshs, Maris; Hinrichsen, Hans-Harald; Elferts, D.

    2015-01-01

    observations during 1969–1995 on four stations in each of two transects. The oceanographic monitoring and demersal trawl research survey data from 1974–2012 have been used in analyses. Results. The sufficient reproduction conditions in the southern Gotland Basin persisted only until 1981. In later decades...... reproduction volume concept can be used as an ecological indicator for egg survival probability in the Gotland Basin. However, it is too premature to re-define the concept of the suitable reproduction volume because it is applicable only to the Gotland spawning ground...

  6. Elemental detection in muscle of flatfish Achirus Iineatus and Trinectes paulistanus (Actinopterygii, Pleuronectiformes) from Santos Bay, Southeastern Brazilian coast

    International Nuclear Information System (INIS)

    Rocha, Maria Luiza Chiste Flaquer da; Dias, June Ferraz; Dias, Johnny Ferraz

    2013-01-01

    Full text: Studies on the presence of elements, including metals, in organisms can prevent both degradation of aquatic systems and the bioaccumulation of elements especially those that offer risk to human health. The aim of this study was to investigate the presence elements in the muscle of two species of flatfish, Achirus Iineatus and Trinectes paulistanus (Achiridae, Pleuronectiformes), using Particle Induced X-Ray Emission (PIXE). This technique is considered one of the most sensitive for detecting trace metals in biomaterials. These flatfish specimens are considered residents in Santos Bay, central coast of Sao Paulo state, and they were caught monthly throughout the year 2005 and sorted by size and maturity stage as juveniles and adults. The two species have no spatial overlap and this allows a mapping of the Santos Bay for the presence of bioavailable metals and other elements. Sampling was done in six distinct points in the Bay, under different antropic influences: two along the beaches, one in the vicinity of the out fall diffuser and three along the entrance of the bay. Results from 54 samples of muscle allowed detect the following elements: aluminium (AI), arsenic (As), lead (Pb), copper (Cu), chromium (Cr), iron (Fe), strontium (Sr), manganese (Mn), mercury (Hg), nickel (Ni), selenium (Se) and zinc (Zn). There were no correlation with the levels of sediment contamination for most metals analyzed in muscles, with the exception of copper and also no clear pattern of differential level of metals between young and adult specimens of A. Iineatus and T. paulistanus. According to the maximum tolerance values of the Brazilian National Health Surveillance Agency (Agencia Nacional de Vigilancia Sanitaria -ANVISA), fish muscle presented levels of contamination above the permitted for human consumption, mainly for some elements: mercury, in samples from the internal area; arsenic, in samples from west side and selenium, in samples from east side of the bay. (author)

  7. Moderate pathogenic effect of Ligophorus uruguayense (Monogenoidea, Ancyrocephalidae in juvenile mullet Mugil liza (Actinopterygii, Mugilidae from Brazil

    Directory of Open Access Journals (Sweden)

    EDUARDO PAHOR-FILHO

    2017-10-01

    Full Text Available ABSTRACT Monogenoidea pathogenic activity can elicit various histological responses in fish. Species of Ligophorus are specific parasites of mullets, and its relationship with host fish may result in a moderate pathogenic action. In order to ascertain this relationship, estuarine mullets (Mugil liza were collected in an estuary, reared in laboratory, for three weeks, and forwarded for histological and parasitological analyses. Ligophorus uruguayense (Monogenoidea infestation in the gills of the mullets was identified. The severe infestation by only one species of Monogenoidea may result from the specificity of these parasites to mullets. Mullets submitted to histological analysis exhibited respiratory epithelium detachment; mild, moderate and severe hyperplasia of the respiratory epithelium; atrophy; and telangiectasia of the gills. This is the first study reporting that mullets highly infested by Monogenoidea can show mild (100% to severe (20% gill changes with a distinct frequency of occurrence. Because of the high prevalence of mild alterations observed, it is possible to accept that L. uruguayense is moderately pathogenic to M. liza, even during high prevalence and intensity of infestation, as a result of its specificity.

  8. Four new species of Ligophorus (Monogenea: Dactylogyridae) parasitic on Mugil liza (Actinopterygii: Mugilidae) from Guandu River, southeastern Brazil.

    Science.gov (United States)

    Abdallah, Vanessa D; de Azevedo, Rodney K; Luque, José L

    2009-08-01

    Four species of Ligophorus (Monogenea: Dactylogyridae), i.e., L. tainhae n. sp., L. brasiliensis n. sp., L. guanduensis n. sp., and L. lizae n. sp., are described. The specimens were collected from the gills of Mugil liza (Mugilidae) from the Guandu River (22 degrees 48'32"S, 43 degrees 37'35"W), State of Rio de Janeiro, Brazil, between January 2008 and March 2008. The male copulatory organ of L. tainhae n. sp. differs from the all known species of this genus in having the largest accessory piece, the length of accessory piece exceeding the length of the copulatory organ tube, and the distal tip of the lower lobe crossing the upper lobe. Ligophorus brasiliensis n. sp. and L. guanduensis n. sp. have a similar shape of the accessory piece, but in L. guanduensis n. sp. the lower lobe is larger than the upper lobe (as opposed to L. brasiliensis n. sp.), the ratio between length of upper lobe and the length of the proximal part of the accessory piece before the bifurcation is shorter and the distal tip of the lower lobe extends to the level of the upper lobe (in L. brasiliensis n. sp. the distal tip of lower lobe crossing the upper lobe). In L. lizae n. sp., the terminal bifurcations of the accessory piece are equal in length and unequal in the other 3 new species. Species of Ligophorus are recorded for the first time from Brazil.

  9. Moderate pathogenic effect of Ligophorus uruguayense (Monogenoidea, Ancyrocephalidae) in juvenile mullet Mugil liza (Actinopterygii, Mugilidae) from Brazil.

    Science.gov (United States)

    Pahor-Filho, Eduardo; Klosterhoff, Marta C; Marchiori, Natalia C; Pereira, Joaber

    2017-01-01

    Monogenoidea pathogenic activity can elicit various histological responses in fish. Species of Ligophorus are specific parasites of mullets, and its relationship with host fish may result in a moderate pathogenic action. In order to ascertain this relationship, estuarine mullets (Mugil liza) were collected in an estuary, reared in laboratory, for three weeks, and forwarded for histological and parasitological analyses. Ligophorus uruguayense (Monogenoidea) infestation in the gills of the mullets was identified. The severe infestation by only one species of Monogenoidea may result from the specificity of these parasites to mullets. Mullets submitted to histological analysis exhibited respiratory epithelium detachment; mild, moderate and severe hyperplasia of the respiratory epithelium; atrophy; and telangiectasia of the gills. This is the first study reporting that mullets highly infested by Monogenoidea can show mild (100%) to severe (20%) gill changes with a distinct frequency of occurrence. Because of the high prevalence of mild alterations observed, it is possible to accept that L. uruguayense is moderately pathogenic to M. liza, even during high prevalence and intensity of infestation, as a result of its specificity.

  10. The parasite community of round goby Neogobius melanostomus (Pallas, 1814) (Actinopterygii: Gobiidae) newly introduced into the upper Elbe

    Czech Academy of Sciences Publication Activity Database

    Kvach, Yuriy; Ondračková, Markéta; Janáč, Michal; Jurajda, Pavel

    2017-01-01

    Roč. 418, April (2017), č. článku 19. ISSN 1961-9502 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:68081766 Keywords : enemy release hypothesis * Neogobius melanostomus * North Sea basin * parasite spillback * Pomphorhynchus tereticollis Subject RIV: EG - Zoology OBOR OECD: Marine biology, freshwater biology, limnology Impact factor: 1.217, year: 2016

  11. Ancient connections among the European rivers and watersheds revealed from the evolutionary history of the genus Telestes (Actinopterygii; Cypriniformes.

    Directory of Open Access Journals (Sweden)

    Ivana Buj

    Full Text Available In order to better understand the complex geologic history of the Mediterranean area, we have analysed evolutionary history, phylogeographic structure and molecular diversity of freshwater fishes belonging to the genus Telestes. As primary freshwater fishes distributed largely in the Mediterranean basin, this genus represents a suitable model system for investigating the historical biogeography of freshwater drainage systems in southern Europe. In this investigation we have included samples representing all Telestes species and based our analyses on one mitochondrial and one nuclear gene. We have investigated phylogenetic structure inside the genus Telestes, estimated divergence times, reconstructed ancestral distribution ranges and described intraspecific molecular diversity. Diversification of Telestes started in the Early Miocene, when the ancestors of T. souffia, lineage comprising T. croaticus and T. fontinalis, and the one comprising T. pleurobipunctatus and T. beoticus got isolated. The remaining species are genetically more closely related and form a common cluster in the recovered phylogenetic trees. Complex geological history of southern Europe, including formation of continental bridges, fragmentation of landmass, closing of the sea corridor, local tectonic activities, led to complicated biogeographical pattern of this genus, caused by multiple colonization events and passovers between ancient rivers and water basins. Especially pronounced diversity of Telestes found in the Adriatic watershed in Croatia and Bosnia and Herzegovina is a consequence of a triple colonization of this area by different lineages, which led to an existence of genetically distinct species in neighboring areas. Significant intraspecific structuring is present in T. souffia, T. muticellus, T. croaticus and T. pleurobipunctatus. Besides in well-structured species, elevated levels of genetic polymorphism were found inside T. turskyi and T. ukliva, as a consequence of their old origin and unconstrained evolutionary history.

  12. Ancient connections among the European rivers and watersheds revealed from the evolutionary history of the genus Telestes (Actinopterygii; Cypriniformes)

    Science.gov (United States)

    Buj, Ivana; Ćaleta, Marko; Šanda, Radek; Geiger, Matthias F.; Freyhof, Jörg; Machordom, Annie; Vukić, Jasna

    2017-01-01

    In order to better understand the complex geologic history of the Mediterranean area, we have analysed evolutionary history, phylogeographic structure and molecular diversity of freshwater fishes belonging to the genus Telestes. As primary freshwater fishes distributed largely in the Mediterranean basin, this genus represents a suitable model system for investigating the historical biogeography of freshwater drainage systems in southern Europe. In this investigation we have included samples representing all Telestes species and based our analyses on one mitochondrial and one nuclear gene. We have investigated phylogenetic structure inside the genus Telestes, estimated divergence times, reconstructed ancestral distribution ranges and described intraspecific molecular diversity. Diversification of Telestes started in the Early Miocene, when the ancestors of T. souffia, lineage comprising T. croaticus and T. fontinalis, and the one comprising T. pleurobipunctatus and T. beoticus got isolated. The remaining species are genetically more closely related and form a common cluster in the recovered phylogenetic trees. Complex geological history of southern Europe, including formation of continental bridges, fragmentation of landmass, closing of the sea corridor, local tectonic activities, led to complicated biogeographical pattern of this genus, caused by multiple colonization events and passovers between ancient rivers and water basins. Especially pronounced diversity of Telestes found in the Adriatic watershed in Croatia and Bosnia and Herzegovina is a consequence of a triple colonization of this area by different lineages, which led to an existence of genetically distinct species in neighboring areas. Significant intraspecific structuring is present in T. souffia, T. muticellus, T. croaticus and T. pleurobipunctatus. Besides in well-structured species, elevated levels of genetic polymorphism were found inside T. turskyi and T. ukliva, as a consequence of their old origin and unconstrained evolutionary history. PMID:29227999

  13. Pelagic occurrence and diet of invasive round goby Neogobius melanostomus (Actinopterygii, Gobiidae) juveniles in deep well-mixed European reservoirs.

    Czech Academy of Sciences Publication Activity Database

    Jůza, Tomáš; Zemanová, Jana; Tušer, Michal; Sajdlová, Zuzana; Baran, Roman; Vašek, Mojmír; Ricard, Daniel; Blabolil, Petr; Wagenvoort, A. J.; Ketelaars, H. A. M.; Kubečka, Jan

    2016-01-01

    Roč. 768, č. 1 (2016), s. 197-209 ISSN 0018-8158 R&D Projects: GA MŠk(CZ) EE2.3.20.0204; GA MŠk(CZ) EE2.3.30.0032 Institutional support: RVO:60077344 Keywords : Biesbosch reservoirs * fish community composition changes * invasive species * vertical distribution * length distribution * fry trawling Subject RIV: EH - Ecology, Behaviour Impact factor: 2.056, year: 2016

  14. Elemental detection in muscle of flatfish Achirus Iineatus and Trinectes paulistanus (Actinopterygii, Pleuronectiformes) from Santos Bay, Southeastern Brazilian coast

    Energy Technology Data Exchange (ETDEWEB)

    Rocha, Maria Luiza Chiste Flaquer da; Dias, June Ferraz [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Instituto Oceanografico. Laboratorio de Ecologia Reprodutiva e do Recrutamento de Organismos Marinhos; Dias, Johnny Ferraz [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, ES (Brazil). Instituto de Fisica. Laboratorio de Implantacao Ionica

    2013-07-01

    Full text: Studies on the presence of elements, including metals, in organisms can prevent both degradation of aquatic systems and the bioaccumulation of elements especially those that offer risk to human health. The aim of this study was to investigate the presence elements in the muscle of two species of flatfish, Achirus Iineatus and Trinectes paulistanus (Achiridae, Pleuronectiformes), using Particle Induced X-Ray Emission (PIXE). This technique is considered one of the most sensitive for detecting trace metals in biomaterials. These flatfish specimens are considered residents in Santos Bay, central coast of Sao Paulo state, and they were caught monthly throughout the year 2005 and sorted by size and maturity stage as juveniles and adults. The two species have no spatial overlap and this allows a mapping of the Santos Bay for the presence of bioavailable metals and other elements. Sampling was done in six distinct points in the Bay, under different antropic influences: two along the beaches, one in the vicinity of the out fall diffuser and three along the entrance of the bay. Results from 54 samples of muscle allowed detect the following elements: aluminium (AI), arsenic (As), lead (Pb), copper (Cu), chromium (Cr), iron (Fe), strontium (Sr), manganese (Mn), mercury (Hg), nickel (Ni), selenium (Se) and zinc (Zn). There were no correlation with the levels of sediment contamination for most metals analyzed in muscles, with the exception of copper and also no clear pattern of differential level of metals between young and adult specimens of A. Iineatus and T. paulistanus. According to the maximum tolerance values of the Brazilian National Health Surveillance Agency (Agencia Nacional de Vigilancia Sanitaria -ANVISA), fish muscle presented levels of contamination above the permitted for human consumption, mainly for some elements: mercury, in samples from the internal area; arsenic, in samples from west side and selenium, in samples from east side of the bay. (author)

  15. A practical approach to phylogenomics: the phylogeny of ray-finned fish (Actinopterygii as a case study

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    Zhang Gong

    2007-03-01

    Full Text Available Abstract Background Molecular systematics occupies one of the central stages in biology in the genomic era, ushered in by unprecedented progress in DNA technology. The inference of organismal phylogeny is now based on many independent genetic loci, a widely accepted approach to assemble the tree of life. Surprisingly, this approach is hindered by lack of appropriate nuclear gene markers for many taxonomic groups especially at high taxonomic level, partially due to the lack of tools for efficiently developing new phylogenetic makers. We report here a genome-comparison strategy to identifying nuclear gene markers for phylogenetic inference and apply it to the ray-finned fishes – the largest vertebrate clade in need of phylogenetic resolution. Results A total of 154 candidate molecular markers – relatively well conserved, putatively single-copy gene fragments with long, uninterrupted exons – were obtained by comparing whole genome sequences of two model organisms, Danio rerio and Takifugu rubripes. Experimental tests of 15 of these (randomly picked markers on 36 taxa (representing two-thirds of the ray-finned fish orders demonstrate the feasibility of amplifying by PCR and directly sequencing most of these candidates from whole genomic DNA in a vast diversity of fish species. Preliminary phylogenetic analyses of sequence data obtained for 14 taxa and 10 markers (total of 7,872 bp for each species are encouraging, suggesting that the markers obtained will make significant contributions to future fish phylogenetic studies. Conclusion We present a practical approach that systematically compares whole genome sequences to identify single-copy nuclear gene markers for inferring phylogeny. Our method is an improvement over traditional approaches (e.g., manually picking genes for testing because it uses genomic information and automates the process to identify large numbers of candidate makers. This approach is shown here to be successful for fishes, but also could be applied to other groups of organisms for which two or more complete genome sequences exist, which has important implications for assembling the tree of life.

  16. Parasite communities and infection levels of the invasive Chinese sleeper Perccottus glenii (Actinopterygii: Odontobutidae) from the Naab river basin, Germany

    Czech Academy of Sciences Publication Activity Database

    Kvach, Yuriy; Janáč, Michal; Nehring, S.; Ondračková, Markéta; Jurajda, Pavel

    2017-01-01

    Roč. 91, č. 6 (2017), s. 703-710 ISSN 0022-149X R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:68081766 Keywords : Amur sleeper * Baltic Sea Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 1.420, year: 2016

  17. Fish, Actinopterygii, Perciformes, Gobiidae, Amblyeleotris rubrimarginata Mohlmann and Randall, 2002: Range gap filled in northern Borneo

    OpenAIRE

    Townsend, D.

    2010-01-01

    The present work reports the shrimp goby Amblyeleotris rubrimarginata Mohlmann and Randall, 2002 in coralsand habitats off three different islands within the Tunku Abdul Rahman marine Park (TARP) off western Sabah in northernBorneo.

  18. Population structure, condition, and reproduction characteristics of native monkey goby, Neogobius fluviatilis (Actinopterygii: Perciformes Gobiidae), in the Bulgarian Danube

    Czech Academy of Sciences Publication Activity Database

    Konečná, Markéta; Jurajda, Pavel

    2012-01-01

    Roč. 42, č. 4 (2012), s. 321-327 ISSN 0137-1592 R&D Projects: GA ČR(CZ) GAP505/11/1768 Institutional support: RVO:68081766 Keywords : monkey goby * invasion * Bulgaria * Danube, condition * GSI * ovary structure Subject RIV: EH - Ecology, Behaviour Impact factor: 0.606, year: 2012

  19. SPATIAL STRUCTURE OF HELMINTH COMMUNITIES IN THE GOLDEN GREY MULLET, LIZA AURATA (ACTINOPTERYGII: MUGILIDAE), FROM THE WESTERN MEDITERRANEAN

    Czech Academy of Sciences Publication Activity Database

    Míguez-Lozano, R.; Pardo-Carranza, T.V.; Blasco-Costa, Maria Isabel; Balbuena, J. A.

    2012-01-01

    Roč. 98, č. 5 (2012), s. 904-912 ISSN 0022-3395 Institutional support: RVO:60077344 Keywords : 1914 DIGENEA HAPLOPORIDAE * PARASITE SPECIES RICHNESS * N. SP MONOGENEA * MARINE FISHES * ENVIRONMENTAL COMPONENTS * LIGOPHORUS MONOGENEA * SATURNIUS MANTER * BETA DIVERSITY * DISTANCE DECAY * BLACK-SEA Subject RIV: EH - Ecology, Behaviour Impact factor: 1.321, year: 2012 http://www.bioone.org/doi/abs/10.1645/GE-2897.1#fn2

  20. Evolutionary history of the fish genus Astyanax Baird & Girard (1854 (Actinopterygii, Characidae in Mesoamerica reveals multiple morphological homoplasies

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    Doadrio Ignacio

    2008-12-01

    Full Text Available Abstract Background Mesoamerica is one of the world's most complex biogeographical regions, mostly due to its complex geological history. This complexity has led to interesting biogeographical processes that have resulted in the current diversity and distribution of fauna in the region. The fish genus Astyanax represents a useful model to assess biogeographical hypotheses due to it being one of the most diverse and widely distributed freshwater fish species in the New World. We used mitochondrial and nuclear DNA to evaluate phylogenetic relationships within the genus in Mesoamerica, and to develop historical biogeographical hypotheses to explain its current distribution. Results Analysis of the entire mitochondrial cytochrome b (Cytb gene in 208 individuals from 147 localities and of a subset of individuals for three mitochondrial genes (Cytb, 16 S, and COI and a single nuclear gene (RAG1 yielded similar topologies, recovering six major groups with significant phylogeographic structure. Populations from North America and Upper Central America formed a monophyletic group, while Middle Central America showed evidence of rapid radiation with incompletely resolved relationships. Lower Central America lineages showed a fragmented structure, with geographically restricted taxa showing high levels of molecular divergence. All Bramocharax samples grouped with their sympatric Astyanax lineages (in some cases even with allopatric Astyanax populations, with less than 1% divergence between them. These results suggest a homoplasic nature to the trophic specializations associated with Bramocharax ecomorphs, which seem to have arisen independently in different Astyanax lineages. We observed higher taxonomic diversity compared to previous phylogenetic studies of the Astyanax genus. Colonization of Mesoamerica by Astyanax before the final closure of the Isthmus of Panama (3.3 Mya explains the deep level of divergence detected in Lower Central America. The colonization of Upper Mesoamerica apparently occurred by two independent routes, with lineage turnover over a large part of the region. Conclusion Our results support multiple, independent origins of morphological traits in Astyanax, whereby the morphotype associated with Bramocharax represents a recurrent trophic adaptation. Molecular clock estimates indicate that Astyanax was present in Mesoamerica during the Miocene (~8 Mya, which implies the existence of an incipient land-bridge connecting South America and Central America before the final closure of the Isthmus of Panama (~3.3 Mya.

  1. Morphometric variation between two morphotypes within the Astyanax Baird and Girard, 1854 (Actinopterygii: Characidae) genus, from a Mexican tropical lake.

    Science.gov (United States)

    Ornelas-García, Claudia P; Bastir, Markus; Doadrio, Ignacio

    2014-07-01

    Phenotypic variation is important for evolutionary processes because it can allow local adaptation, promote genetic segregation, and ultimately give rise to speciation. Lacustrine systems provide a unique opportunity to study the mechanisms by which sister species can co-occur by means of ecological segregation. The fish genus Astyanax is characterized by high levels of phenotypic variability, providing an excellent model for the study of local specialization. Here, we analyze the morphological specializations through geometric morphometrics of two sympatric species described as different genera: Bramocharax caballeroi endemic to Lake Catemaco, and the widely distributed Astyanax aeneus. Additionally, we assess the correlation between phenotypic and genetic structure, and the phylogenetic signal of morphological variation. We examined body size and shape variation in 196 individuals and analyzed mitochondrial cytochrome b sequences in 298 individuals. Our results confirm the striking morphological divergence among the sympatric characids. Differences between them were mainly found in the body depth and profile and orientation of the head, where B. caballeroi in contrast with the A. aeneus, presented a fusiform body and an upward mouth. Moreover, different growth trajectories were observed among morphotypes, suggesting that a heterochronic process could be involved in the diversification of our study system. Morphological differences did not correspond with the molecular differentiation, suggesting high levels of homoplasy among the lineages of B. caballeroi morphs. © 2014 Wiley Periodicals, Inc.

  2. A new grenadier of the genus Hymenocephalus from Tosa Bay, southern Japan (Actinopterygii: Gadiformes: Macrouridae)

    DEFF Research Database (Denmark)

    Nakayama, Naohide; Endo, Hiromitsu; Schwarzhans, Werner

    2015-01-01

    Hymenocephalus yamasakiorum, a new species of grenadier fishes, is described from a single specimen collected from Tosa Bay off Aki, Kochi, Japan, at a depth of 320–400 m. The species is distinguished from its 26 known congeners in having the following combination of features: pelvic-fin rays nine......; pectoral-fin rays i16–i17; first dorsal-fin rays II, 10; chin barbel 55 % of head length (HL), its tip extending beyond the hind margin of orbit when laid back; snout moderately high, not depressed, length 28 % HL; orbit diameter 33 % HL; interorbital width 24 % HL; gill rakers on first arch (outer...

  3. Estimation of grey seal (Halichoerus grypus diet composition in the Baltic Sea

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    Karl Lundström

    2007-01-01

    Full Text Available We examined the digestive tract contents from 145 grey seals (Halichoerus grypus collected between 2001 and 2004 in the Baltic Sea. We compensated for biases introduced by erosion of otoliths, both by using additional hard-part structures other than otoliths, and species-specific size and numerical correction factors. In the absence of numerical correction factors based on feeding experiments for some species, we used correction factors based on a relationship between otolith recoveryrate and otolith width. A total of 24 prey taxa were identified but only a few species contributed substantially to the diet. The estimated diet composition was, independently of the prey number estimation method and diet composition estimation model used, dominated by herring (Clupea harengus, both by numbers and biomass. In addition to herring, common whitefish (Coregonus lavaretus and sprat (Sprattus sprattus were important prey, but cyprinids (Cyprinidae, eelpout (Zoarces viviparus, flounder (Platichtys flesus and salmon (Salmo salar also contributed significantly. Our results indicated dietary differences between grey seals of different age as well as between seals from the northern (Gulf of Bothnia and the southern (Baltic Proper Baltic Sea.

  4. SCARDINIUS GENUS IN MOLECULAR STUDIES – A REVIEW

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    Ovidiu Popescul

    2015-12-01

    Full Text Available Scardinius is a genus of ray-finned fish in the Cyprinidae family commonly called rudds. The common rudd(Scardinius erithrophthalmus is a bentho-pelagic freshwater fish that occurs mainly in nutrient-rich, well vegetatedlowland rivers, backwaters, oxbows, ponds and lakes and it is widespread in Europe and middle Asia. It has a mediumlength of 20-30 cm, but it can reach 50 cm. The classification of cyprinids has always been controversial the morphologicaltraits have an unclear homology this led to the idea that the recognized monophyletic groups are surely misinterpreted. Thispaper aims to assess the current level of molecular data regarding Scardinius genera. Some of the molecular data obtainedfor Scardinius genus is from DNA barcoding studies on fresh water fishes, but studies regarding this genus and Cyprinidaefamily used mitochondrial genes like cytochrome b (cyt b and cytochrome oxidase (CO, but nuclear genes or nuclearmicrosatellites were also used. We found that molecular data exists for both nuclear and mitochondrial genes, but this genuswasn’t studied separately and as many of the researchers suggest more taxonomic studies are required in order to solve theuncertainties within it.

  5. The post-larval and juvenile fish assemblage in the Sukhothai floodplain, Thailand

    Science.gov (United States)

    Siriwan, Suksri; Boonsatien, Boonsoong

    2017-06-01

    This study investigated abundance, species composition and spatial and temporal distributions of fish larvae and their relationship with some environmental variables in the Sukhothai floodplain in northern Thailand. Fish larvae were collected from 33 sampling stations on 8 occasions between August 2010 and October 2013. The study collected and identified 149 296 individuals, representing 32 families and 165 taxa. The species composition of larval fish was dominated by the Cyprinidae (47.27%), Cobitidae (7.88%), Siluridae (6.67%), Bagridae (6.06%) and Mastacembelidae (3.33%) families. The most-abundant larval species were the Striped flying barb Esomus metallicus (16.90%), the Siamese mud carp Henicorhynchus siamensis (8.48%) and the Sumatran river sprat Clupeichthys goniognathus (8.31%). The greatest abundance and species diversity of larvae were found when the river flow runs onto the floodplain. PCA and nMDS analysis revealed that the samples plot is associated with temporal distribution among years. The discharge was a major factor determining fish larvae assemblage and environmental variables in the Sukhothai floodplain. Four fish larval species were positively correlated with the samples for 2013. The result of the CCA ordination plot showed that only the discharge variable was strongly correlated with fish larvae abundance, especially two cyprinid Rasbora species.

  6. Variation in male reproductive traits among three bitterling fishes (Acheilognathinae: Cyprinidae) in relation to the mating system

    Czech Academy of Sciences Publication Activity Database

    Pateman-Jones, C.; Rasotto, M. B.; Reichard, Martin; Liao, C.; Liu, H.; Zieba, G.; Smith, C.

    2011-01-01

    Roč. 103, č. 3 (2011), s. 622-632 ISSN 0024-4066 Institutional research plan: CEZ:AV0Z60930519 Keywords : reproduction * sperm competition * sperm depletion * sperm economy Subject RIV: EG - Zoology Impact factor: 2.193, year: 2011

  7. Reproductive strategies of two sympatric 'small barbs'(Barbus humilis and B. tanapelagius, Cyprinidae)in Lake Tana, Ethiopia

    NARCIS (Netherlands)

    Dejen, E.; Sibbing, F.A.; Vijverberg, J.

    2003-01-01

    The reproductive strategies of two species of 'small barbs' (12,000 fish). Both species have a long breeding period (from March to September), and the distinct bimodal size-frequency distributions of eggs suggest multiple spawning for both species. Absolute fecundity increased exponentially with

  8. Molecular phylogeny of north mediterranean freshwater barbs (genus Barbus: cyprinidae) inferred from cytochrome b sequences: biogeographic and systematic implications.

    Science.gov (United States)

    Tsigenopoulos, C S; Berrebi, P

    2000-02-01

    We investigated phylogenetic relationships among north Mediterranean species of the genus Barbus using sequences of the cytochrome b gene. Our results indicate that the species belong to two major clades that are consistent with those previously defined from morphological features. The first clade includes species ranging from France to the Black Sea. In this clade, there is a well-supported monophyletic group of large-sized fluvio-lacustrine barbs; however, the monophyly of the small-sized rheophilic species is not clear. The second clade comprises species found in Spain, Greece, and Asia Minor and probably represents the oldest group present in the north Mediterranean rivers. In general, there is good concordance between geography and phylogenetic relationships. These results are compared to those from previous morphological- and allozyme-based studies and demonstrate widespread discordance and polyphyly in the traditional taxonomy of the genus Barbus. This study is one of the first reporting the phylogenetic and biogeographic relationships of a genus that is widely distributed in European rivers and contains species that are a major component of the European ichthyofauna. Copyright 2000 Academic Press.

  9. Effect of temperature on the effectiveness of artificial reproduction of dace [Cyprinidae (Leuciscus leuciscus (L.))] under laboratory and field conditions.

    Science.gov (United States)

    Nowosad, Joanna; Targońska, Katarzyna; Chwaluczyk, Rafał; Kaszubowski, Rafał; Kucharczyk, Dariusz

    2014-10-01

    This study sought to determine the effect of water temperature on the effectiveness of artificial reproduction of dace brooders under laboratory and field conditions. Three temperatures were tested in the laboratory: 9.5, 12 and 14.5 °C (± 0.1 °C). The water temperature under field conditions was 11.0 ± 0.3 °C (Czarci Jar Fish Farm) and 13.2 ± 1.4 °C (Janowo Fish Farm). The study showed that artificial reproduction of dace is possible in all the temperature ranges under study and an embryo survival rate of over 87% can be achieved. Dace has also been found to be very sensitive to rapid temperature changes, even within the temperature ranges optimal for the species. Such changes have an adverse effect on the outcome of the reproduction process, such as a decrease in the percentage of reproducing females, a decrease in the pseudo-gonado-somatic index (PGSI) and a decrease in the embryo survival rate. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Assessment of heavy metal load in chub liver (Cyprinidae – Leuciscus cephalus from the Nišava River (Serbia

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    Jovanović, B.

    2011-09-01

    Full Text Available The Nišava River plays an important role as the source for both drinking water and agricultural irrigation due to its hydrological and geomorphological characteristics as the largest river in the region of southeast Serbia. In this study we used the liver of the chub (Leiciscus cephalus as a tool for biomonitoring heavy metal accumulation along the river. Chub specimens were sampled from two localities (one at the border with Bulgaria and a second in the city of Niš. Concentrations were estimated for six heavy metals (iron, cadmium, copper, zinc, lead and manganese in chub liver. Low bioconcentration level was observed for most of the metals and the concentrations corresponded to the nominal concentration in livers of fish inhabiting metal unpolluted streams and rivers. However, cadmium concentration in the chub liver exceeded 0.5 mg kg-1, a several hundred folds increase from nominal concentration indicating a potential toxic exposure of the fish and of the stream ecosystem to this heavy metal. Hepatosomatic indices were calculated and tested for the impact of metal concentrations on liver size. A decrease of the hepatic index was observed in fish with higher cadmium concentration, suggesting a possible impact on the health of the chub population in the Nišava River.

  11. Population genetic characterization and family reconstruction in brood bank collections of the Indian major carp Labeo rohita (Cyprinidae:Cypriniformes).

    Science.gov (United States)

    Ullah, Ashraf; Basak, Abhisak; Islam, Md Nazrul; Alam, Md Samsul

    2015-01-01

    The founder stock of a captive breeding program is prone to changes in genetic structure due to inbreeding and genetic drift. Genetic characterization of the founder population using suitable molecular markers may help monitor periodic changes in the genetic structure in future. To develop benchmark information about the genetic structure we analyzed six microsatellite loci in the Brodbank collections of rohu (Labeo rohita) originated from three major rivers-the Jamuna, the Padma and the Halda. A total of 28 alleles were detected in 90 individuals with an average of 4.6 alleles per locus. The average observed heterozygosity ranged from 0.655 to 0.705 and the expected heterozygosity ranged from 0.702 to 0.725. The mean F IS values were 0.103, 0.106 and 0.018 for the Jamuna, Padma and Halda fishes respectively. The population pair-wise F ST values ranged from 0.0057 to 0.0278. Structure analysis grouped the fishes of the three rivers into two clusters. The numbers of half-sib families were 5, 5 and 4 and the numbers of full-sib families were 12, 10 and 18 for the Halda, Jamuna and the Padma samples respectively. Bottleneck was detected in all the river samples. We recommend to collect more fish from different locations of the major rivers to broaden the genetic variability of the founder stocks of the Brood bank.

  12. Detection of morphometric differentiation in Sattar snowtrout, Schizothorax curvifrons (Cypriniformes: Cyprinidae) from Kashmir Himalaya using a truss network system.

    Science.gov (United States)

    Mir, Farooq Ahmad; Mir, Javaid Iqbal; Chandra, Suresh

    2014-03-01

    Schizothorax curvifrons is a morphometrically and meristically most variable and economically valuable promising fish food from Kashmir Valley. Since there is a lack of information on stock structure of wild populations on this species, this study was aimed to investigate the intraspecific variation of this important snowtrout. For this, two rivers and one lake in Kashmir Himalaya were sampled from January 2011 to October 2012. Fish body measurements were taken and morphometric characters using the truss network system was constructed. Altogether, 506 fish specimens were collected. Data were subjected to principal component analysis, discriminant function analysis and univariate analysis of variance. The first principal component explained 63.44% of total variation, while second and third components explained 8.34% and 5.31%, respectively. The step-wise discriminant function analysis retained two variables that significantly discriminated the populations. Using these variables 83.4% of the original specimens were classified into their correct groups and 81.1% of the cross-validated (leave one out procedure) specimens were classified into their correct groups. All of the total 31 transformed truss measurements exhibited highly significant (pHimalaya area.

  13. Setting Conservation Priorities in a Widespread Species: Phylogeographic and Physiological Variation in the Lake Chub, Couesius plumbeus (Pisces: Cyprinidae

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    Patricia M. Schulte

    2013-04-01

    Full Text Available Defining units of conservation below the species level is a widely accepted conservation priority, but is especially challenging for widespread taxa that have experienced diverse geographic histories and exist across heterogenous environments. The lake chub (Pisces: Couesius plumbeus is a widespread freshwater fish in North America and occurs from the southcentral USA to northwestern Alaska and Canada. We used mtDNA sequence analysis to test for divergent lineages predicted to occur as a result of survival of lake chub in distinct glacial refugia. Lake chub consisted of two major mtDNA lineages separated by 3.8% sequence divergence which are probably late to pre-Pleistocene in origin. We combined these data with those consistent with thermal adaptation in fish living in thermal springs versus those living in a lake with wide seasonal temperature variation, and with data on distribution of lake chub in major watershed units. We assessed these data against objective criteria developed to identify conservation units under Canadian endangered species legislation. Our analysis identified twelve major units of conservation within C. plumbeus that could be assessed under Canada’s Species-at-Risk Act. Our study illustrates how different character traits manifested at very different spatial scales can be used to define conservation units within widely-distributed taxa.

  14. Mapping the potential distribution of the invasive Red Shiner, Cyprinella lutrensis (Teleostei: Cyprinidae) across waterways of the conterminous United States

    Science.gov (United States)

    Poulos, Helen M.; Chernoff, Barry; Fuller, Pam L.; Butman, David

    2012-01-01

    Predicting the future spread of non-native aquatic species continues to be a high priority for natural resource managers striving to maintain biodiversity and ecosystem function. Modeling the potential distributions of alien aquatic species through spatially explicit mapping is an increasingly important tool for risk assessment and prediction. Habitat modeling also facilitates the identification of key environmental variables influencing species distributions. We modeled the potential distribution of an aggressive invasive minnow, the red shiner (Cyprinella lutrensis), in waterways of the conterminous United States using maximum entropy (Maxent). We used inventory records from the USGS Nonindigenous Aquatic Species Database, native records for C. lutrensis from museum collections, and a geographic information system of 20 raster climatic and environmental variables to produce a map of potential red shiner habitat. Summer climatic variables were the most important environmental predictors of C. lutrensis distribution, which was consistent with the high temperature tolerance of this species. Results from this study provide insights into the locations and environmental conditions in the US that are susceptible to red shiner invasion.

  15. Variability and condition of common minnow (Phoxinus phoxinus, Cyprinidae, Cypriniformes) on the Semipalatinsk range and out of its influence

    International Nuclear Information System (INIS)

    Mitrofanov, I.V.; Matmuratov, S.A.

    2003-01-01

    Populations of common minnow (Phoxinus phoxinus) from five rivers of Irtysh and Balkhash basins were investigated. Population from the Shagan river situated inside area of Semipalatinsk Range influence. This population has normal sexual structure and high growth rate. Condition factor of all fishes is high. Population from Semipalatinsk Range has increased variability of characteristics due to increasing of stochastic part of general variability. Abnormalities of external fish morphology are not observed in this area. Asymmetry of bilateral characteristics is low. (author)

  16. Distribution of Phoxinus eos, Phoxinus neogaeus, and their asexually-reproducing hybrids (Pisces: Cyprinidae in Algonquin Provincial Park, Ontario.

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    Jonathan A Mee

    Full Text Available Hybrid Phoxinus are one of the few asexually reproducing vertebrates species. The distribution of hybrid Phoxinus among lakes in Algonquin Park, Ontario, was evaluated relative to the distribution of parental species and relative to physiochemical lake characteristics. No association between the distribution of hybrids and the distribution of parental species was found, suggesting that the hybrids can successfully coexist with either parental species. In addition, we found no association between hybrid distribution and the physiochemical characteristics of lakes, suggesting that the hybrids are generalists with respect to the ecological niches available in Algonquin Park. There was a difference between the physiochemical characteristics of lakes with and without the parental species P. neogaeus. The lakes containing P. neogaeus were lower elevation than the lakes containing the other parental species, P. eos. The difference in distribution between the parental species may therefore be due to different dispersal abilities, to later arrival following post-glacial dispersal, or to differences in ecology. These results suggest that asexual reproduction is a successful strategy for hybrid Phoxinus in Algonquin Park because these sperm-dependent asexual hybrids are able to survive and persist regardless of which parental species is present, and regardless of the physiochemical characteristics of their habitat.

  17. Chromosomes of Iberian Leuciscinae (Cyprinidae) Revisited: Evidence of Genome Restructuring in Homoploid Hybrids Using Dual-Color FISH and CGH

    Czech Academy of Sciences Publication Activity Database

    Pereira, C. S.; Ráb, Petr; Collares-Pereira, M. J.

    2013-01-01

    Roč. 141, 2/3 (2013), s. 143-152 ISSN 1424-8581 R&D Projects: GA ČR GA13-37277S Institutional support: RVO:67985904 Keywords : CGH/GISH * Chondrostoma s.I. * genome reshuffling hybridization Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.905, year: 2013

  18. Postglacial recolonization of eastern Blacknose Dace,Rhinichthys atratulus(Teleostei: Cyprinidae), through the gateway of New England.

    Science.gov (United States)

    Tipton, Michelle L; Gignoux-Wolfsohn, Sarah; Stonebraker, Phoebe; Chernoff, Barry

    2011-11-01

    During the last ice age, much of North America far south as 40°N was covered by glaciers (Hewitt 2000). About 20,000 years ago, as the glaciers retreated, the hydrologic landscape changed dramatically creating waterways for fish dispersal. The number of populations responsible for recolonization and the regions from which they recolonized are unknown for many freshwater fishes living in New England and southeastern Canada. The Blacknose Dace,Rhinichthys atratulus, is one of the freshwater fish species that recolonized this region. We hypothesize that the earliest deglaciated region, modern-day Connecticut, was recolonized byR. atratulusvia a single founding event by a single population. In this paper, we test this hypothesis phylogenetically with regard to the major drainage basins within Connecticut. The mitochondrial DNA exhibits low nucleotide diversity, high haplotype diversity, and a dominant haplotype found across the state. A small percentage of individuals in the Housatonic drainage basin, however, share a haplotype with populations in New York drainage basins, a haplotype not found elsewhere in Connecticut's drainage basins. We calculated a range for the rate of divergence for NADH dehydrogenase subunit 2 (nd2) and control region (ctr) of 4.43-6.76% and 3.84-8.48% per million years (my), respectively. While this range is higher than the commonly accepted rate of 2% for mitochondrial DNA, these results join a growing list of publications finding high rates of divergence for various taxa (Peterson and Masel 2009). The data support the conclusion that Connecticut as a whole was recolonized initially by a single founding event that came from a single refugium. Subsequently, the Housatonic basin alone experienced a secondary recolonization event.

  19. Histological and allometric growth analysis of eye in Caspian kutum, Rutilus kutum Kamensky, 1901 (Teleostei: Cyprinidae during early developmental stages

    Directory of Open Access Journals (Sweden)

    Shaghayegh Hasanpour

    2016-10-01

    Full Text Available Fish larvae have several sensory systems that are functional at or soon after hatching and then are developed further during larval and juvenile stages. This study was conducted to investigate development of the eye in Rutilus kutum, based on histological and allometric growth analysis during early developmental stages up to 35 day post hatching with emphasis on retinal morphology. For this purpose, the histological sections were prepared and allometric growth pattern of the eye was calculated. The results showed that the most eye’s structures along with the retina of the newly hatched larvae, as the inner sensory (photosensitive tissue were completely differentiated. Allometric growth pattern of the eye diameter up to the inflexion point (7 dph was somewhat positive and then it became negative. The results revealed that the Caspian kutum is dependence on visual capability as visual feeder during their larval period which itself explains completion of eye structures and the high growth rate of eye before 3 dph i.e. beginning of mixed feeding.

  20. The analysis of the pharyngeal-sieve mechanism and the efficiency of food intake in the bream (Abramis brama, Cyprinidae)

    NARCIS (Netherlands)

    Hoogenboezem, W.

    1991-01-01

    This thesis describes the mechanism and the selectivity of food intake in bream ( Abramis brama ). It is a compilation of six articles which have been published (or will soon be published) in international journals.

    In the first chapter, diets and feeding modes in

  1. The role of the European bitterling (Rhodeus amarus, Cyprinidae) in parasite accumulation and transmission in riverine ecosystems

    Czech Academy of Sciences Publication Activity Database

    Dávidová, M.; Blažek, Radim; Trichkova, T.; Koutrakis, E.; Gaygusuz, Ö.; Ercan, E.; Ondračková, Markéta

    2011-01-01

    Roč. 45, č. 3 (2011), s. 377-387 ISSN 1386-2588 R&D Projects: GA AV ČR KJB600930802; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60930519 Keywords : European bitterling * Freshwater habitat * Metazoan parasites * Parasite transmission * Intermediate host Subject RIV: EH - Ecology, Behaviour Impact factor: 1.365, year: 2011

  2. Comparative cytogenetics of two endangered leuciscine fish, Squalius aradensis and S. torgalensis (Teleostei, Cyprinidae, from the Iberian Peninsula

    Directory of Open Access Journals (Sweden)

    Catarina Nabais

    2013-03-01

    Full Text Available In this study, the description of the karyotypes of the endangered chubs Squalius aradensis (Coelho, Bogutskaya, Rodrigues and Collares-Pereira, 1998 and Squalius torgalensis (Coelho, Bogutskaya, Rodrigues and Collares-Pereira, 1998 is presented by means of conventional (Giemsa-staining, Chromomycin A3 (CMA3-fluorescence, Silver-impregnation (Ag-NORs and molecular (fluorescence in situ hybridization (FISH with 18S rDNA probe protocols. These endemic sister-species have an allopatric but adjacent distribution in the most southwestern part of the Iberian Peninsula. Diploid chromosome number was invariably 2n = 50 and karyotypes of both species were grossly similar, composed of metacentric and submetacentric elements with a reduced number of acrocentric pairs. Sequential staining using FISH with an 18S rDNA probe, CMA3 and Ag-NORs treatments revealed consistent positive signals located at the end of the short arms of a submetacentric chromosome pair, likely homologous in both species. While providing useful cytogenetic comparative data against other members of the genus Squalius Bonaparte, 1837, the work aimed to draw attention towards the conservation of two narrow-range and highly confined fish species.

  3. Occurrence of the Kessler’s gudgeon Romanogobio kesslerii (Dybowski, 1862) (Cyprinidae) in the Upper Vistula River (Poland)

    Czech Academy of Sciences Publication Activity Database

    Nowak, M.; Klaczak, A.; Szczerbik, P.; Mendel, Jan; Popek, W.

    2014-01-01

    Roč. 30, č. 5 (2014), s. 1062-1064 ISSN 0175-8659 R&D Projects: GA ČR GP206/09/P608 Institutional support: RVO:68081766 Keywords : Kessler's gudgeon * Upper Vistula River Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.867, year: 2014

  4. Complete mitochondrial genome of threatened mahseer Tor tor (Hamilton 1822) and its phylogenetic relationship within Cyprinidae family.

    Science.gov (United States)

    Pavan-Kumar, A; Raman, Sudhanshu; Koringa, Prakash G; Patel, Namrata; Shah, Tejas; Singh, Rajeev K; Krishna, Gopal; Joshi, C G; Gireesh-Babu, P; Chaudhari, Aparna

    2016-12-01

    The mahseers (Tor, Neolissochilus and Naziritor) are an important group of fishes endemic to Asia with the conservation status of most species evaluated as threatened. Conservation plans to revive these declining wild populations are hindered by unstable taxonomy. Molecular phylogeny studies with mitochondrial genome have been successfully used to reconstruct the phylogenetic tree and to resolve taxonomic ambiguity. In the present study, complete mitochondrial genome of Tor tor has been sequenced using ion torrent next-generation sequencing platform with coverage of more than 1000 x. Comparative mitogenome analysis shows higher divergence value at ND1 gene than COI gene. Further, occurrence of a distinct genetic lineage of T. tor is revealed. The phylogenetic relationship among mahseer group has been defined as Neolissochilus hexagonolepis ((T. sinensis (T. putitora, T. tor), (T. khudree, T. tambroides)).

  5. A multilocus assessment of nuclear and mitochondrial sequence data elucidates phylogenetic relationships among European spirlins (Alburnoides, Cyprinidae)

    Czech Academy of Sciences Publication Activity Database

    Stierandová, Soňa; Vukic, J.; Vasil'eva, E. D.; Zogaris, S.; Shumka, S.; Halačka, Karel; Vetešník, Lukáš; Švátora, M.; Nowak, M.; Stefanov, T.; Koščo, J.; Mendel, Jan

    2016-01-01

    Roč. 94, January (2016), s. 479-491 ISSN 1055-7903 Grant - others:GA AV ČR(CZ) M200930901 Institutional support: RVO:68081766 Keywords : Alburnoides * Taxonomy * Phylogeography * Mitochondrial and nuclear markers Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 4.419, year: 2016

  6. Dynamics of Rex3 in the genomes of endangered Iberian Leuciscinae (Teleostei, Cyprinidae) and their natural hybrids

    Czech Academy of Sciences Publication Activity Database

    Pereira, C. S.; Pazian, M. F.; Ráb, Petr; Collares-Pereira, M. J.

    2015-01-01

    Roč. 8, č. 81 (2015) ISSN 1755-8166 Institutional support: RVO:67985904 Keywords : Anaecypris hispanica * Chondrostoma s.l. sp * Karyotype differentiation Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.506, year: 2015

  7. A new species of Rhabdochona Railliet, 1916 (Nematoda: Rhabdochonidae) from cyprinid fishes in the Western Ghats Region, India

    Czech Academy of Sciences Publication Activity Database

    González-Solís, David; Chavan, S. P.; Kannewad, P.; Gyananath, G.

    2014-01-01

    Roč. 87, č. 3 (2014), s. 273-281 ISSN 0165-5752 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : fresh water fishes * morphology * Thailand Subject RIV: EA - Cell Biology Impact factor: 1.336, year: 2014

  8. Rhabdochona (Rhabdochona) hypsibarbi n. sp. (Nematoda: Rhabdochonidae) from the Freshwater Cyprinid Fish Hypsibarbus wetmorei (Smith) in Northeast Thailand

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Pachanawan, A.; Kamchoo, K.

    2013-01-01

    Roč. 99, č. 2 (2013), s. 297-302 ISSN 0022-3395 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Parasitic nematode * Rhabdochona * Thailand Subject RIV: EA - Cell Biology Impact factor: 1.258, year: 2013

  9. Divergence with gene flow between Ponto-Caspian refugia in an anadromous cyprinid Rutilus frisii revealed by multiple gene phylogeography

    Czech Academy of Sciences Publication Activity Database

    Kotlík, Petr; Marková, Silvia; Choleva, Lukáš; Bogutskaya, N. G.; Ekmekci, F. G.; Ivanova, P.

    2008-01-01

    Roč. 17, - (2008), s. 1076-1088 ISSN 0962-1083 R&D Projects: GA AV ČR KJB600450507 Institutional research plan: CEZ:AV0Z50450515 Keywords : Black Sea * climate change * inland waters Subject RIV: EG - Zoology Impact factor: 5.325, year: 2008

  10. The effects of elevated river discharge on the downstream drift of young-of-the-year cyprinid fishes

    Czech Academy of Sciences Publication Activity Database

    Reichard, Martin; Jurajda, Pavel

    2004-01-01

    Roč. 19, č. 3 (2004), s. 465-471 ISSN 0270-5060 R&D Projects: GA AV ČR IAB6093106 Institutional research plan: CEZ:AV0Z6093917 Keywords : larvae * juvenile fish Subject RIV: EG - Zoology Impact factor: 0.374, year: 2004

  11. Revision of Khawia spp. (Cestoda: Caryophyllidea), parasites of cyprinid fish, including a key to their identification and molecular phylogeny

    Czech Academy of Sciences Publication Activity Database

    Scholz, Tomáš; Brabec, Jan; Kraľová-Hromadová, I.; Oros, M.; Bazsalovicsová, E.; Ermolenko, A. S.; Hanzelová, V.

    2011-01-01

    Roč. 58, č. 3 (2011), 197–223 ISSN 0015-5683 R&D Projects: GA ČR GA524/08/0885; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : tapeworms * freshwater fish * comparative morphology * taxonomy * phylogenetic relationships * dentification * DNA sequences * Holarctic Region Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.812, year: 2011 http://www.paru.cas.cz/folia/pdfs/showpdf.php?pdf=21988

  12. Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary.

    Science.gov (United States)

    Doszpoly, Andor; Papp, Melitta; Deákné, Petra P; Glávits, Róbert; Ursu, Krisztina; Dán, Ádám

    2015-05-01

    In the early summer of 2014, mass mortality of sichel (Pelecus cultratus) was observed in Lake Balaton, Hungary. Histological examination revealed degenerative changes within the tubular epithelium, mainly in the distal tubules and collecting ducts in the kidneys and multifocal vacuolisation in the brain stem and cerebellum. Routine molecular investigations showed the presence of the DNA of an unknown alloherpesvirus in some specimens. Subsequently, three genes of the putative herpesviral genome (DNA polymerase, terminase, and helicase) were amplified and partially sequenced. A phylogenetic tree reconstruction based on the concatenated sequence of these three conserved genes implied that the virus belongs to the genus Cyprinivirus within the family Alloherpesviridae. The sequences of the sichel herpesvirus differ markedly from those of the cypriniviruses CyHV-1, CyHV-2 and CyHV-3, putatively representing a fifth species in the genus.

  13. Conflict between morphology and molecular data: a case of the genus Caryophyllaeus (Cestoda: Caryophyllidea), monozoic tapeworms of cyprinid fishes

    Czech Academy of Sciences Publication Activity Database

    Bazsalovicsová, E.; Kráľová-Hromadová, I.; Brabec, Jan; Hanzelová, V.; Oros, M.; Scholz, Tomáš

    2014-01-01

    Roč. 61, č. 4 (2014), s. 347-354 ISSN 0015-5683 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : scolex morphology * morphological plasticity * Caryophyllaeus laticeps * Caryophyllaeus brachycollis * mitochondrial haplotypes * cox1 * lsrDNA * morphotypes Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.147, year: 2014

  14. Association between IL-10a SNPs and resistance to cyprinid herpesvirus-3 infection in common carp (Cyprinus carpio)

    Science.gov (United States)

    Analysis of gene polymorphisms and disease association is essential for assessing putative candidate genes affecting susceptibility or resistance to disease. In this paper, we report the results of an association analysis between SNPs in common carp innate immune response genes and resistance to Cy...

  15. Do laboratory species protect endangered species? Interspecies variation in responses to 17β-estradiol, a model endocrine active compound

    Science.gov (United States)

    Jorgenson, Zachary G.; Buhl, Kevin J.; Bartell, Stephen E.; Schoenfuss, Heiko L.

    2015-01-01

    Although the effects of estrogens on model laboratory species are well documented, their utility as surrogates for other species, including those listed as endangered, are less clear. Traditionally, conservation policies are evaluated based on model organism responses but are intended to protect all species in an environment. We tested the hypothesis that the endangered Rio Grande silvery minnow (Hybognathus amarus) is more vulnerable to endocrine disruption—as assessed through its larval predator-escape performance, survival, juvenile sex ratios, and whole-body vitellogenin concentration—than the commonly used toxicological model species fathead minnow (Pimephales promelas) and the bluegill sunfish (Lepomis macrochirus). Fish were exposed concurrently for 21 days to the model endocrine active compound (EAC) 17ß-estradiol (E2) at 10 ng E2/L and 30 ng E2/L in a flow-through system using reconstituted water that simulated the physicochemical conditions of the Middle Rio Grande in New Mexico, USA. No significant differences were observed between the fathead and silvery minnow in larval predator-escape response or juvenile sex ratio. Rio Grande silvery minnow survival decreased significantly at day 14 compared with the other two species; by day 21, both cyprinid species (silvery minnow and fathead minnow) exhibited a significant decrease in survival compared with bluegill sunfish, a member of the family Centrarchidae. Male Rio Grande silvery minnow showed a significant increase in whole-body vitellogenin concentration in the 10 ng/L treatment, whereas fathead minnow and bluegill sunfish showed no significant increases in vitellogenin concentrations across treatments. Our study showed response differences to estrogen exposures between the two cyprinid species and further divergence in responses between the families Cyprinidae and Centrarchidae. These results suggest that commonly used laboratory model organisms may be less sensitive to EACs than the endangered

  16. Do laboratory species protect endangered species? Interspecies variation in responses to 17β-estradiol, a model endocrine active compound.

    Science.gov (United States)

    Jorgenson, Z G; Buhl, K; Bartell, S E; Schoenfuss, H L

    2015-01-01

    Although the effects of estrogens on model laboratory species are well documented, their utility as surrogates for other species, including those listed as endangered, are less clear. Traditionally, conservation policies are evaluated based on model organism responses but are intended to protect all species in an environment. We tested the hypothesis that the endangered Rio Grande silvery minnow (Hybognathus amarus) is more vulnerable to endocrine disruption-as assessed through its larval predator-escape performance, survival, juvenile sex ratios, and whole-body vitellogenin concentration-than the commonly used toxicological model species fathead minnow (Pimephales promelas) and the bluegill sunfish (Lepomis macrochirus). Fish were exposed concurrently for 21 days to the model endocrine active compound (EAC) 17ß-estradiol (E2) at 10 ng E2/L and 30 ng E2/L in a flow-through system using reconstituted water that simulated the physicochemical conditions of the Middle Rio Grande in New Mexico, USA. No significant differences were observed between the fathead and silvery minnow in larval predator-escape response or juvenile sex ratio. Rio Grande silvery minnow survival decreased significantly at day 14 compared with the other two species; by day 21, both cyprinid species (silvery minnow and fathead minnow) exhibited a significant decrease in survival compared with bluegill sunfish, a member of the family Centrarchidae. Male Rio Grande silvery minnow showed a significant increase in whole-body vitellogenin concentration in the 10 ng/L treatment, whereas fathead minnow and bluegill sunfish showed no significant increases in vitellogenin concentrations across treatments. Our study showed response differences to estrogen exposures between the two cyprinid species and further divergence in responses between the families Cyprinidae and Centrarchidae. These results suggest that commonly used laboratory model organisms may be less sensitive to EACs than the endangered Rio

  17. Variation in life-history traits between a newly established and long-established population of non-native pumpkinseed, Lepomis gibbosus (Actinopterygii: Perciformes: Centrarchidae)

    Czech Academy of Sciences Publication Activity Database

    Konečná, Markéta; Janáč, Michal; Roche, Kevin Francis; Jurajda, Pavel

    2015-01-01

    Roč. 45, č. 4 (2015), s. 385-392 ISSN 0137-1592 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:68081766 Keywords : freshwater fish * alien species * introduced species * date of introduction * size-at-maturity * fecundity Subject RIV: EH - Ecology, Behaviour Impact factor: 0.622, year: 2015

  18. Different Histories, Different Destinies‒Impact of Evolutionary History and Population Genetic Structure on Extinction Risk of the Adriatic Spined Loaches (Genus Cobitis; Cypriniformes, Actinopterygii.

    Directory of Open Access Journals (Sweden)

    Ivana Buj

    Full Text Available The region of Balkans is often considered as an ichthyologic "hot spot", with a great number of species and high portion of endemics living in fresh waters in a relatively small area. The Adriatic watershed in Croatia and Herzegovina is inhabited by six spined loach species (genus Cobitis whose extinction risk estimations were based solely on their extent of occurrence (and/or area of occupancy and its fragmentation, and conservation proposals do not consider diversity below species level. In this investigation we employed molecular genetic methods to describe present genetic structure of the Adriatic spined loaches and reveal their demographic history. The divergence of the Adriatic lineages inside the genus Cobitis started in Miocene and lasted until Pleistocene epoch. Geological events responsible for shaping recent diversity of spined loaches in the Adriatic basin are: the Dinarid Mountains upwelling, the evolution of Dinaric Lake system, local tectonic activity, river connections during glaciations and differences in sea level. Even though all the investigated species inhabit karstic rivers located in the same geographic area and that were subject of similar geological events, the results obtained reveal great differences in their genetic diversity and structure and point out the necessity of different conservation measures to ensure their future viability. High level of genetic polymorphism is characteristic for species located more to the south. Two species comprised of more than one population have completely different intraspecific structure; populations of C. illyrica are genetically distinct and represent separate evolutionary significant units, whereas intraspecific structure of C. narentana corresponds to metapopulational pattern. Without population genetic data, evolutionary significant units could be easily misidentified. Furthermore, the obtained results affirm that population genetic measurements are able to detect differences among closely located and related species and estimate extinction risk even more accurately than currently applied IUCN criteria.

  19. Comparison of the bioaccumulation from seawater and depuration of heavy metals and radionuclides in the spotted dogfish Scyliorhinus canicula (Chondrichthys) and the turbot Psetta maxima (Actinopterygii: Teleostei)

    International Nuclear Information System (INIS)

    Jeffree, Ross A.; Warnau, Michel; Teyssie, Jean-Louis; Markich, Scott J.

    2006-01-01

    The bioaccumulation of selected heavy metals and radionuclides ( 241 Am, 109 Cd, 57 Co, 51 Cr, 134 Cs, 54 Mn and 65 Zn) from seawater was experimentally compared in the Chondrichthyan Scyliorhinus canicula (spotted dogfish) and the Actinopterygian Teleost Psetta maxima (turbot), of comparable size, age and benthic feeding habits. The speciation of these elements in seawater (salinity 38 per mille , pH 8.1, temperature 16.5 deg. C) was also calculated to determine their potential bioavailability. The uptake rates, measured over 14 days, varied greatly among isotopes and between species. Concentration factors (CFs) in P. maxima varied 5-fold between ca. 0.2 for 51 Cr and 2.5 for 65 Zn and 134 Cs, whereas in S. canicula they varied by a much greater factor of 350, with CFs for 51 Cr and 241 Am ranging from ca. 0.4 to 140, respectively. With the exception of 134 Cs, all radiotracers were accumulated at a faster rate in S. canicula than in P. maxima, particularly for 241 Am and 65 Zn where the CFs attained during the uptake phase were, two and one order of magnitude greater in S. canicula, respectively. In contrast, 134 Cs reached a CF of about 2.5 in P. maxima, which was 5-fold greater than in S. canicula. Patterns of loss from the experimental depuration phase over 29 days showed greater similarities between species, compared to the uptake phase that highlighted the greater differences between elements. The distributions of these seven radioisotopes among six body components indicated that between the two species the skin of the dogfish displayed a greater bioaccumulation potential, particularly for 241 Am, 57 Co and 65 Zn. However 65 Zn was also distinctive from 241 Am and 57 Co in its pattern of bioaccumulation in dogfish, with its other body components attaining concentrations of 65 Zn that were comparable to the levels found in its skin. The heightened uptake of 134 Cs in turbot was characterised by a more even percentage distribution among its tissues compared to 241 Am and 57 Co, but every tissue of turbot had a higher concentration of 134 Cs, compared to dogfish, particularly the muscle and liver. The elevated uptake rates and higher CFs for most radioisotopes indicate that S. canicula is more susceptible than P. maxima to exposure and contamination by these metals and radionuclides in seawater. These experimentally-determined differences between dogfish and turbot in their bioaccumulation characteristics were assessed against a set of criteria erected to evaluate the working hypothesis that they were taxonomically based. The outcomes of this initial assessment were supportive of this hypothesis that warrants further investigation

  20. First record of the silver-cheeked toad fish Lagocephalus scleratus (Gmelin, 1789 (Actinopterygii: Tetraodontidae from Chennai coastal waters, Southeast India

    Directory of Open Access Journals (Sweden)

    Krishnan Silambarasan

    2017-04-01

    Full Text Available The silver-cheeked toad fish, Lagocephalus scleratus, was recorded for the first time on 25 September 2014. Two specimens of this fish species were collected from the by-catch landed by a commercial deep-sea trawler at Kasimedu Fishing Harbour, Chennai coast, Southeast India. The morphometric and meristic characters of the recorded specimens are described and discussed. The specimen was compared with earlier reports.

  1. Composição em tamanho dos peixes (Actinopterygii, Teleostei de ribeirões da bacia do rio Tibagi, Paraná, Brasil

    Directory of Open Access Journals (Sweden)

    Shibatta Oscar A.

    2003-01-01

    Full Text Available The standard length of 7610 specimens of 43 species from eight streams of rio Tibagi basin was obtained in order to analyze the means of fish sizes. The higher mean size was observed in Gymnotus carapo Linnaeus, 1758 (165,5 mm and the smaller, in Phalloceros caudimaculatus (Hensel, 1868 (16,25 mm. Some fish like Acestrorhynchus lacustris (Reinhardt, 1874, Leporinus obtusidens (Valenciennes, 1836, Gymnotus carapo Linneus, 1758 and Synbranchus marmoratus Bloch, 1795 can reach big or medium size when adults but were collected only young, evidencing they use some streams in beginning of life. The mean size of total species was 49,81mm, what shows the small size composition of fishes in the streams. The Kolmogov-Smirnov test comparing the mean size of all streams showed significant similar size both in upstream and downstream regions, independent to the different environmental conditions.

  2. Age, growth, and reproductive aspects of the dusky grouper Mycteroperca marginata (Actinopterygii: Epinephelidae in a man-made rocky habitat in southern Brazil

    Directory of Open Access Journals (Sweden)

    Elisa Seyboth

    2011-01-01

    Full Text Available The dusky grouper, Mycteroperca marginata, is an important commercial marine fish that is currently considered an endangered species worldwide and listed as overexploited in Brazil. Although its reproductive biology has been studied elsewhere, no information is available for populations in its southernmost distribution limit on the Brazilian coast. The present work investigates age structure, growth and reproduction of individuals inhabiting a pair of rocky jetties, about 4.5 km long each, located in the mouth of Patos Lagoon in the municipality of Rio Grande, in the southwestern Atlantic Ocean. A total of 130 individuals obtained between February 2007 and May 2009 were analyzed, with total length ranging from 260 to 800 mm. The growth coefficient found in the present study (K: 0.069 was lower than values reported for dusky grouper populations from the Mediterranean Sea (0.087 and southeast Africa (0.09. This difference could be related to higher average water temperatures in these regions compared to the current study site or due to poorer habitat quality in terms of the shelters available for dusky grouper individuals to establish their territories. Microscopic ovarian analyses of individuals during three years of sampling revealed only the occurrence of immature and resting females. The absence of ripe or post-spawning individuals, associated with low GSI values (A garoupa-verdadeira, Mycteroperca marginata, é uma espécie de importância comercial que atualmente é considerada como ameaçada mundialmente e sobre-explotada no Brasil. Embora sua biologia reprodutiva tenha sido estudada em outras regiões, nenhuma informação está disponível sobre populações que habitam o extremo sul da sua distribuição na costa brasileira. O presente trabalho investiga a estrutura de idade, crescimento e reprodução de indivíduos que habitam dois molhes de pedra, com cerca de 4,5 km de extensão cada, localizados na desembocadura da laguna dos Patos no município de Rio Grande, no Atlântico Sudoeste. Foram analisados 130 indivíduos obtidos entre fevereiro de 2007 e maio de 2009, com comprimento total entre 260 e 800 mm. O coeficiente de crescimento no presente estudo (K: 0,069 foi inferior aos valores reportados para oMediterrâneo (0,087 e sudeste da África (0,09. Essa diferença pode estar relacionada a temperaturas médias maiores nessas regiões comparadas ao presente local de estudo ou devido a menor qualidade de habitat em termo de abrigos para os indivíduos de garoupa-verdadeira estabelecerem seus territórios. Análises microscópicas dos ovários dos indivíduos durante esses três anos de amostragens revelaram somente a ocorrência de indivíduos imaturos e fêmeas em repouso. A ausência de indivíduos ovados ou em pós-desova, associada com baixos valores do IGS (<0,2, ao longo de todos os meses de coleta, indica que a garoupa-verdadeira não utiliza esse substrato rochoso construído pelo homem como um local de desova. A estimativa de um L50 de 451,3 mm indica que a maioria dos indivíduos capturados nessa região é imatura. Pressão de pesca sobre indivíduos imaturos, associada com a sua lenta taxa de crescimento e complexo modo reprodutivo, ameaça a sustentabilidade da atual pesca da garoupa-verdadeira na área de estudo.

  3. Role of the invasive Chinese sleeper Perccottus glenii (Actinopterygii: Odontobutidae) in the distribution of fish parasites in Europe: New data and a review

    Czech Academy of Sciences Publication Activity Database

    Kvach, Yuriy; Kutsokon, Y.; Stepien, C. A.; Markovych, M.

    2016-01-01

    Roč. 71, č. 8 (2016), s. 941-951 ISSN 0006-3088 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:68081766 Keywords : Chinese sleeper * enemy release hypothesis * invasive species * parasites * Perccottus glenii Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.759, year: 2016

  4. Bioacumulation of trace elements in hepatic and renal tissues of the white mullet Mugil curema Valenciennes, 1836 (Actinopterygii, Mugilidae) in two coastal systems in southeastern Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez, W.S., E-mail: fernandez@usp.br [Graduate Program in Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanográfico 191, Cidade Universitária, CEP 05508-120 São Paulo, SP (Brazil); Physics Institute, Federal University of Rio Grande do Sul, CP 15051, CEP 91501-970 Porto Alegre, RS (Brazil); Dias, J.F. [Oceanographic Institute, University of São Paulo, Praça do Oceanográfico 191, Cidade Universitária, CEP 05508-120 São Paulo, SP (Brazil); Boufleur, L.A.; Amaral, L.; Yoneama, M.L.; Dias, J.F. [Physics Institute, Federal University of Rio Grande do Sul, CP 15051, CEP 91501-970 Porto Alegre, RS (Brazil)

    2014-01-01

    The aim of this study is to investigate the presence and the concentration of trace elements in hepatic and renal tissues of white mullet (Mugil curema) by Particle-induced X-ray emission (PIXE). Fish specimens were collected in two coastal areas of São Paulo state-Brazil: the Santos estuary (from March 2009 to February 2010) and the Cananéia-Iguape coastal estuarine system (from May 2008 to April 2009). For the elemental analysis, n = 470 sample tissues (liver and kidney) were pooled according to location and type of organ. Trace elements such as Fe, Cu, Zn and Br were observed in both tissues of M. curema with concentrations ranging from 800 μg g{sup −1} for Fe to 7 μg g{sup −1} for Cu. The concentrations of Cu and Zn showed statistical significant differences among the tissues of M. curema (p < 0.05). Relatively higher concentrations of Cu and Zn were observed in the liver tissue. There was no significantly difference in the elemental concentrations between the two studied areas. The Cu levels in liver tissues of M. curema were found to be above the maximum limits for consumption, according to the United States Environmental Protection Agency (EPA) and Brazilian National Health Surveillance Agency (ANVISA)

  5. [THE MICROSPORIDIUM GLUGEA GASTEROSTEI VORONIN 1974 (MICROSPORIDIA: MARINOSPORIDIA) FROM THE THREE-SPINED STICKLEBACK GASTEROSTEUS ACULEATUS (ACTINOPTERYGII: GASTEROSTEIFORMES) AS AN INDEPENDENT SPECIES].

    Science.gov (United States)

    Tokarev, Y S; Voronin, V N; Senderskiy, I V; Issi, I V

    2015-01-01

    The microsporidium Glugea gasterostei from the three-spined stickleback Gasterosteus aculeatus was described as an independent species basing upon morphological and ecological traits of the parasite (Voronin, 1974), further supported by ultrastructural characters of its spores (Voronin, 1983). During the revision of microsporidia of the genus Glugea (Canning, Lom, 1986; Lom, 2002), the validity of this species was doubted and it was synonymized with G. anomala. Nevertheless, the molecular phylogenetic analysis performed in the present study showed the unique molecular haplotype of small subunit rRNA gene of G. gasterostei (Genbank accession number KM977990) and its close relatedness to G. anomala, G. atherinae and G. hertwigi (sequence similarity of 99.7 %). One of typical characters of G. gasterostei, as opposed to G. anomala, is the formation of xenomas on inner tissues and not on the surface of infected fishes. This feature is retained even after the infection of different host species. Taken together, these data confirm the validity of G. gasterostei as a separate species among closely related taxa that had diverged comparatively recently.

  6. First record of the Chinese sleeper, Perccottus glenii Dybowski, 1877 (Actinopterygii: Odontobutidae) in the Dnieper Estuary, southern Ukraine (Black Sea drainage)

    Czech Academy of Sciences Publication Activity Database

    Kvach, Yuriy; Dykyy, I.; Janko, Karel

    2016-01-01

    Roč. 5, č. 4 (2016), s. 285-290 E-ISSN 2242-1300 R&D Projects: GA ČR(CZ) GBP505/12/G112; GA ČR GA13-12580S Institutional support: RVO:68081766 ; RVO:67985904 Keywords : invasive species * new finding * Dnieper Estuary * Ukraine Subject RIV: EH - Ecology, Behaviour Impact factor: 0.835, year: 2016 http://www.reabic.net/journals/bir/2016/4/BIR_2016_Kvach_etal.pdf

  7. Coeficientes de digestibilidad aparente de harina de pescado peruana y maíz amarillo duro para Colossoma macropomum (Actinopterygii, Characidae

    Directory of Open Access Journals (Sweden)

    Felix Walter Gutierrez

    2008-12-01

    Full Text Available Los Coeficientes de Digestibilidad Aparente (CDA de la materia seca (MS, proteína cruda (PC, lípido crudo(LC y energía bruta (EB de los ingredientes alimenticios harina de pescado peruana (HPP y de maíz amarillo duro (MAD fueron determinados en juveniles de Colossoma macropomum (150,0 ± 25,5 g. En el experimento la dieta de referencia se mezcló con cada uno de los ingredientes prueba en una relación de 70:30. El óxido crómico se usó como indicador inerte. La dieta de referencia y las dietas prueba fueron suministradas a C. macropomum, criada a 27 ºC, 7 mg/L de oxígeno disuelto y pH entre 7,5. Las muestras fecales fueron colectadas por sifoneo. Los CDA para MS, PC, LC y EB de la HPP fueron de 88,06 ± 0,83%, 87,08 ± 1,34%, 85,87 ± 2,69 y 87,29 ± 1,57% respectivamente. Igualmente los CDA para MS, PC, LC y EB del MAD fueron de 82,38 ± 1,02%, 75,46 ± 1,53%, 76,17 ± 2,43% y 75,04 ± 1,80% respectivamente. La energía digestible aparente calculada fue de 3950 Kcal/kg para la HPP y 2830 kcal/kg para el MAD. Se concluye que C. macropomum digiere mejor las fracciones proteicas y energéticas de la HPP. Al mismo tiempo muestra que digiere también la fracción energética del MAD, proveniente de los carbohidratos solubles.

  8. Bioacumulation of trace elements in hepatic and renal tissues of the white mullet Mugil curema Valenciennes, 1836 (Actinopterygii, Mugilidae) in two coastal systems in southeastern Brazil

    Science.gov (United States)

    Fernandez, W. S.; Dias, J. F.; Boufleur, L. A.; Amaral, L.; Yoneama, M. L.; Dias, J. F.

    2014-01-01

    The aim of this study is to investigate the presence and the concentration of trace elements in hepatic and renal tissues of white mullet (Mugil curema) by Particle-induced X-ray emission (PIXE). Fish specimens were collected in two coastal areas of São Paulo state-Brazil: the Santos estuary (from March 2009 to February 2010) and the Cananéia-Iguape coastal estuarine system (from May 2008 to April 2009). For the elemental analysis, n = 470 sample tissues (liver and kidney) were pooled according to location and type of organ. Trace elements such as Fe, Cu, Zn and Br were observed in both tissues of M. curema with concentrations ranging from 800 μg g-1 for Fe to 7 μg g-1 for Cu. The concentrations of Cu and Zn showed statistical significant differences among the tissues of M. curema (p < 0.05). Relatively higher concentrations of Cu and Zn were observed in the liver tissue. There was no significantly difference in the elemental concentrations between the two studied areas. The Cu levels in liver tissues of M. curema were found to be above the maximum limits for consumption, according to the United States Environmental Protection Agency (EPA) and Brazilian National Health Surveillance Agency (ANVISA).

  9. Bioacumulation of trace elements in hepatic and renal tissues of the white mullet Mugil curema Valenciennes, 1836 (Actinopterygii, Mugilidae) in two coastal systems in southeastern Brazil

    International Nuclear Information System (INIS)

    Fernandez, W.S.; Dias, J.F.; Boufleur, L.A.; Amaral, L.; Yoneama, M.L.; Dias, J.F.

    2014-01-01

    The aim of this study is to investigate the presence and the concentration of trace elements in hepatic and renal tissues of white mullet (Mugil curema) by Particle-induced X-ray emission (PIXE). Fish specimens were collected in two coastal areas of São Paulo state-Brazil: the Santos estuary (from March 2009 to February 2010) and the Cananéia-Iguape coastal estuarine system (from May 2008 to April 2009). For the elemental analysis, n = 470 sample tissues (liver and kidney) were pooled according to location and type of organ. Trace elements such as Fe, Cu, Zn and Br were observed in both tissues of M. curema with concentrations ranging from 800 μg g −1 for Fe to 7 μg g −1 for Cu. The concentrations of Cu and Zn showed statistical significant differences among the tissues of M. curema (p < 0.05). Relatively higher concentrations of Cu and Zn were observed in the liver tissue. There was no significantly difference in the elemental concentrations between the two studied areas. The Cu levels in liver tissues of M. curema were found to be above the maximum limits for consumption, according to the United States Environmental Protection Agency (EPA) and Brazilian National Health Surveillance Agency (ANVISA)

  10. The complete mitochondrial genome of the Chinese hook snout carp Opsariichthys bidens (Actinopterygii: Cypriniformes) and an alternative pattern of mitogenomic evolution in vertebrate

    DEFF Research Database (Denmark)

    Wang, Xuzhen; Wang, Jun; He, Shunping

    2007-01-01

    The complete mitochondrial genome sequence of the Chinese hook snout carp, Opsariichthys bidens, was newly determined using the long and accurate polymerase chain reaction method. The 16,611-nucleotide mitogenome contains 13 protein-coding genes, two rRNA genes (12S, 16S), 22 tRNA genes, and a no......The complete mitochondrial genome sequence of the Chinese hook snout carp, Opsariichthys bidens, was newly determined using the long and accurate polymerase chain reaction method. The 16,611-nucleotide mitogenome contains 13 protein-coding genes, two rRNA genes (12S, 16S), 22 tRNA genes...

  11. Combining morphology and genetics in resolving taxonomy--a systematic revision of spined loaches (Genus Cobitis; Cypriniformes, Actinopterygii in the Adriatic watershed.

    Directory of Open Access Journals (Sweden)

    Ivana Buj

    Full Text Available Taxonomic investigation of spined loaches from Dalmatia and Herzegovina was conducted on specimens from 14 localities. The results of the detailed morphological investigations were combined with genetic data (based on one mitochondrial and two nuclear genes in order to resolve the taxonomic status of each Cobitis population. Among the investigated features of external morphology, the appearance of spots on the caudal fin base turned out to have the greatest diagnostic value. Furthermore, the number of branched fin rays enabled the discrimination of several species. No morphometric character alone could ensure determination of any Cobitis species. Nevertheless, groups of populations that are more similar in their body shapes correspond to mitochondrial phylogenetic lineages. Based on molecular genetic markers, Dalmatian and Herzegovinian spined loaches form independent lineages inside the Adriatic phylogenetic group. Mitochondrial DNA phylogenetic reconstruction revealed six monophyletic lineages, corresponding to six species distributed in the investigated area. The population distributed in Mostarsko blato karstic field in Bosnia and Herzegovina is described as a new species based on a unique combination of morphological characters: a single triangular Canestrini scale; usually 51/2 branched anal fin rays, 61/2 branched dorsal fin rays, 14 branched caudal fin rays; no spots in the surface pigmentation layer on the caudal fin base; scales on the body very small.

  12. A revision of the Australian species of Trimma (Actinopterygii, Gobiidae), with descriptions of six new species and redescriptions of twenty-three valid species.

    Science.gov (United States)

    Winterbottom, Richard; Hoese, Douglass F

    2015-03-17

    The gobiid genus Trimma currently contains 75 valid species, with another 20-30 known but undescribed species. There are 29 species in Australian waters (six undescribed). This paper describes the six new species, and provides redescriptions of most of the 23 previously described species known from the region, as well as a key for all the species. The six new species are: T. insularum (endemic to Cocos (Keeling) Islands), T. kitrinum (Fiji to Great Barrier Reef), T. meristum (Cape York to the Bismark Archipelago and Fiji), T. pentherum (Great Barrier Reef to Fiji and the South-West Islands of Palau), T. readerae (Australia to Japan), and T. xanthum (Palau to Fiji, Great Barrier Reef to Christmas Island). The following 23 species have been recorded from Australian waters, and most are redescribed here: T. anaima (Comores to Fiji), T. annosum (Maldives to the Phoenix Islands, Taiwan to the southern Great Barrier Reef), T. benjamini (southern Vietnam to the Marshall Islands, Samoa and southern Barrier Reef), T. caesiura (Ryukyus through the Marshall Islands to Samoa and Elizabeth Reef on the Lord Howe Rise), T. capostriatum (New Caledonia to eastern Australia and Papua New Guinea), T. maiandros (Java to the Ryukyus, Marshalls to Great Barrier Reef), T. emeryi (Comores to Ryukyus and Samoa), T. fangi (western South China Sea through to the Solomons), T. flavatrum (Ryukyu Islands to Western Australia and Samoa), T. hoesei (Chagos Archipelago, central Indian Ocean to Palau and Solomons), T. lantana (Australia, Solomons, northern New Guinea, South-West Islands of Palau), T. macrophthalmus (Ryukyu Islands to Cocos (Keeling) Islands and Samoa), T. milta (Taiwan to Western Australia, Society Islands and Hawaii), T. nasa (Sumbawa, Indonesia to Fiji), T. necopinum (northern tip of Cape York to Sydney), T. nomurai (Japan to northern Australia and New Caledonia), T. okinawae (western Thailand to Japan and the Phoenix Islands, north-west Australia to the Great Barrier Reef), T. preclarum (Palau to Fiji, Great Barrier Reef), T. stobbsi (Maldives to New Caledonia), T. striatum (Maldives to Palau, to northern Australia), T. taylori (Red Sea to Hawaii and Society Islands), T. tevegae (Red Sea to Ryukyu Islands, Marshall Islands to Samoa), and T. unisquame (Comores to Hawaii and Easter Island).

  13. Topological congruence between phylogenies of Anacanthorus spp. (Monogenea: Dactylogyridae) and their Characiformes (Actinopterygii) hosts: A case of host-parasite cospeciation.

    Science.gov (United States)

    da Graça, Rodrigo J; Fabrin, Thomaz M C; Gasques, Luciano S; Prioli, Sônia M A P; Balbuena, Juan A; Prioli, Alberto J; Takemoto, Ricardo M

    2018-01-01

    Cophylogenetic studies aim at testing specific hypotheses to understand the nature of coevolving associations between sets of organisms, such as host and parasites. Monogeneans and their hosts provide and interesting platform for these studies due to their high host specificity. In this context, the objective of the present study was to establish whether the relationship between Anacanthorus spp. with their hosts from the upper Paraná River and its tributaries can be explained by means of cospeciation processes. Nine fish species and 14 monogenean species, most of them host specific, were studied. Partial DNA sequences of the genes RAG1, 16S and COI of the fish hosts and of the genes ITS2, COI and 5.8S of the parasite species were used for phylogenetic reconstruction. Maximum likelihood phylogenetic trees of the host and parasite species were built and used for analyses of topological congruence with PACo and ParaFit. The program Jane was used to estimate the nature of cospeciation events. The comparison of the two phylogenies revealed high topological congruence between them. Both PACo and ParaFit supported the hypothesis of global cospeciation. Results from Jane pointed to duplications as the most frequent coevolutionary event, followed by cospeciation, whereas duplications followed by host-switching were the least common event in Anacanthorus spp. studied. Host-sharing (spreading) was also identified but only between congeneric host species.

  14. Growth differences in different biotypes of the hybrid complex of Cobitis elongatoides x Cobitis tanaitica (Actinopterygii: Cypriniformes: Cobitidae) in the Okna River (Danube River basin), Slovakia

    Czech Academy of Sciences Publication Activity Database

    Fedorčák, J.; Koščo, J.; Halačka, Karel; Manko, P.

    2017-01-01

    Roč. 47, č. 2 (2017), s. 125-132 ISSN 0137-1592 R&D Projects: GA ČR GA13-12580S Institutional support: RVO:68081766 Keywords : annulus * back-calculated length * loach * clone * polyploidy Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 0.670, year: 2016

  15. Phylogenetic analysis of molecular and morphological data highlights uncertainty in the relationships of fossil and living species of Elopomorpha (Actinopterygii: Teleostei).

    Science.gov (United States)

    Dornburg, Alex; Friedman, Matt; Near, Thomas J

    2015-08-01

    Elopomorpha is one of the three main clades of living teleost fishes and includes a range of disparate lineages including eels, tarpons, bonefishes, and halosaurs. Elopomorphs were among the first groups of fishes investigated using Hennigian phylogenetic methods and continue to be the object of intense phylogenetic scrutiny due to their economic significance, diversity, and crucial evolutionary status as the sister group of all other teleosts. While portions of the phylogenetic backbone for Elopomorpha are consistent between studies, the relationships among Albula, Pterothrissus, Notacanthiformes, and Anguilliformes remain contentious and difficult to evaluate. This lack of phylogenetic resolution is problematic as fossil lineages are often described and placed taxonomically based on an assumed sister group relationship between Albula and Pterothrissus. In addition, phylogenetic studies using morphological data that sample elopomorph fossil lineages often do not include notacanthiform or anguilliform lineages, potentially introducing a bias toward interpreting fossils as members of the common stem of Pterothrissus and Albula. Here we provide a phylogenetic analysis of DNA sequences sampled from multiple nuclear genes that include representative taxa from Albula, Pterothrissus, Notacanthiformes and Anguilliformes. We integrate our molecular dataset with a morphological character matrix that spans both living and fossil elopomorph lineages. Our results reveal substantial uncertainty in the placement of Pterothrissus as well as all sampled fossil lineages, questioning the stability of the taxonomy of fossil Elopomorpha. However, despite topological uncertainty, our integration of fossil lineages into a Bayesian time calibrated framework provides divergence time estimates for the clade that are consistent with previously published age estimates based on the elopomorph fossil record and molecular estimates resulting from traditional node-dating methods. Copyright © 2015 Elsevier Inc. All rights reserved.

  16. The phylogenetic intrarelationships of spiny-rayed fishes (Acanthomorpha, Teleostei, Actinopterygii: fossil taxa increase the congruence of morphology with molecular data

    Directory of Open Access Journals (Sweden)

    Donald Davesne

    2016-11-01

    Full Text Available Acanthomorpha (spiny-rayed fishes is a clade of teleosts that includes more than 15 000 extant species. Their deep phylogenetic intrarelationships, first reconstructed using morphological characters, have been extensively revised with molecular data. Moreover, the deep branches of the acanthomorph tree are still largely unresolved, with strong disagreement between studies. Here, we review the historical propositions for acanthomorph deep intrarelationships and attempt to resolve their earliest branching patterns using a new morphological data matrix compiling and revising characters from previous studies. The taxon sampling we use constitutes a first attempt to test all previous hypotheses (molecular and morphological alike with morphological data only. Our sampling also includes Late Cretaceous fossil taxa, which yield new character state combinations that are absent in extant taxa. Analysis of the complete morphological data matrix yields a new topology that shows remarkable congruence with the well-supported molecular results. Lampridiformes (oarfishes and allies are the sister to all other acanthomorphs. Gadiformes (cods and allies and Zeiformes (dories form a clade with Percopsiformes (trout-perches and the enigmatic Polymixia (beardfish and Stylephorus (tube-eye. Ophidiiformes (cusk-eels and allies and Batrachoidiformes (toadfishes are nested within Percomorpha, the clade that includes most of modern acanthomorph diversity. These results provide morphological synapomorphies and independent corroboration of clades previously only recovered from molecular data, thereby suggesting the emergence of a congruent picture of acanthomorph deep intrarelationships. Fossil taxa play a critical role in achieving this congruence, since a very different topology is found when they are excluded from the analysis.

  17. Finding of Pseudobacciger harengulae (Digenea, Faustulidae) in the Mediterranean Horse Mackerel, Trachurus mediterraneus (Actinopterygii, Carangidae), from the Gulf of Odessa, Black Sea, Ukraine

    Czech Academy of Sciences Publication Activity Database

    Hulak, B.; Kvach, Yuriy

    2018-01-01

    Roč. 52, č. 1 (2018), s. 47-52 ISSN 0084-5604 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:68081766 Keywords : Black Sea * Digenea * Pseudobacciger * Trachurus * new records * new host Subject RIV: EG - Zoology OBOR OECD: Zoology

  18. The impact of Manjil and Tarik dams (Sefidroud River, southern Caspian Sea basin on morphological traits of Siah Mahi Capoeta gracilis (Pisces: Cyprinidae

    Directory of Open Access Journals (Sweden)

    Adeleh Heidari

    2013-08-01

    Full Text Available It has been postulated that the building of the Manjil and Tarik dams on Sefidroud River has led to the body shape variation of Capoeta gracilis in up- and downstream populations due to the isolation. In this study, Geometric morphometric approach was used to explore body shape variations of Capoeta gracilis populations in up- and downstream Manjil and Tarik dams in Sefidroud River from south of the Caspian Sea basin. The shape of 90 individuals from three sampling sites was extracted by recording the 2-D coordinates of 13 landmark points. PCA, CVA, DFA and CA analysis were used to examine shape differences among the populations. The significant differences were found among the shape of the populations and these differences were observed in the snout, the caudal peduncle and head. The present study indicated the body shape differences in the populations of Capoeta gracilis in the Sefidroud River across the Manjil and Tarik dams, probably due to the dam construction showing anthropogenic transformation of rivers influences body shape in an aquatic organism.

  19. Age and growth of European barbel Barbus barbus (Cyprinidae in the small, mesotrophic River Lee and relative to other populations in England

    Directory of Open Access Journals (Sweden)

    Vilizzi L.

    2013-08-01

    Full Text Available Suspected of being in decline, the European barbel Barbus barbus population of the River Lee, a heavily-modified river in South East England, has been the subject of investigations to identify factors associated with perceived population decreases. Population surveys between 1995 and 1999 captured a total of 912 individuals, and standard length (SL frequency analyses between years suggested that the population decline was not related to juvenile recruitment but rather to a recruitment bottleneck in fish 300–340 mm SL. This bottleneck probably results from insufficient available habitat suitable to this size class. Of the sampled fish, scales were removed from 764 and were used in a scale ageing exercise among three researchers. Analyses of their independent age estimates revealed variable interpretations, which arose from uncertainties relating to the difficulty of analysing scale patterns from relatively large, slow-growing fish. Nevertheless, error was within published acceptable margins, and age estimates revealed B. barbus in the river to age 10 years, lower than in many UK rivers. The SL-at-age growth curve was characterised by very fast growth in the initial years of life. Thus, the causal factors in the decline of this B. barbus population appear to have been in the adult life-stage habitat and were likely related to the loss of longitudinal connectivity, mainly due to the presence of water retention structures. River and aquatic ecosystem remediation strategies should therefore focus on enhancing longitudinal connectivity in conjunction with the ongoing improvement of water quality and ecosystem integrity.

  20. Rapid range expansion of the "whitefin" gudgeon Romanogobio cf. belingi (Teleostei: Cyprinidae) in a lowland tributary of the Vistula River (Southeastern Poland)

    Czech Academy of Sciences Publication Activity Database

    Nowak, M.; Klaczak, A.; Szczerbik, P.; Mendel, Jan; Popek, W.

    2013-01-01

    Roč. 49, č. 4 (2013), s. 319-326 ISSN 0003-4088 R&D Projects: GA ČR GP206/09/P608 Institutional support: RVO:68081766 Keywords : faunistic * Gobioninae * ichthyofauna monitoring * population dynamics * rare species Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.036, year: 2013

  1. A new monozoic tapeworm, Lobulovarium longiovatum n. g., n. sp. (Cestoda: Caryophyllidea), from barbs Puntius spp. (Teleostei: Cyprinidae) in the Indomalayan region

    Czech Academy of Sciences Publication Activity Database

    Oros, Mikuláš; Ash, Anirban; Brabec, Jan; Kar, P. K.; Scholz, Tomáš

    2012-01-01

    Roč. 83, č. 1 (2012), s. 1-13 ISSN 0165-5752 R&D Projects: GA ČR GD206/09/H026; GA ČR GA524/08/0885; GA ČR GBP505/12/G112 Institutional research plan: CEZ:AV0Z60220518 Keywords : MIXED MODELS * SILURIFORMES * PARASITES * IDENTIFICATION * CATOSTOMIDAE * MORPHOLOGY * CESTOIDEA * REVISION * SOPHORE * PISCES Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.260, year: 2012 http://link.springer.com/article/10.1007%2Fs11230-012-9367-6

  2. Growth, biomass, and production of two small barbs (Barbus humilis and B. tanapelagius, Cyprinidae) and their role in the food web of Lake Tana (Ethiopia)

    NARCIS (Netherlands)

    Dejen, E.; Vijverberg, J.; Nagelkerke, L.A.J.; Sibbing, F.A.

    2009-01-01

    Growth, biomass and production of two small barbs (Barbus humilis and Barbus tanapelagius) and their role in the food web of Lake Tana were investigated. From length–frequency distribution of trawl monitoring surveys growth coefficient, F' values were estimated at 3.71–4.17 for B. humilis and

  3. Growth, biomass, and production of two small barbs (Barbus humilis and B. tanapelagius, Cyprinidae) and their role in the food web of Lake Tana (Ethiopia)

    NARCIS (Netherlands)

    Dejen, E.; Vijverberg, J.; Nagelkerke, L.A.J.; Sibbing, F.A.

    2009-01-01

    Growth, biomass and production of two small barbs (Barbus humilis and Barbus tanapelagius) and their role in the food web of Lake Tana were investigated. From length–frequency distribution of trawl monitoring surveys growth coefficient, Φ′ values were estimated at 3.71–4.17 for B. humilis and

  4. Growth performance and feed utilization of keureling fish Tor tambra (Cyprinidae fed formulated diet supplemented with enhanced probiotic. [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Zainal A. Muchlisin

    2017-02-01

    Full Text Available Background The objective of the present study was to determine the optimum dosage of probiotic in the diet of keureling fish (Tor tambra fry. Methods Lactobacillus casei from Yakult® was used as a starter, and enhanced with Curcuma xanthorrhiza, Kaempferia galanga and molasses. The mixture was fermented for 7 days prior to use as probiotic in a formulated diet containing 30% crude protein. Four levels of probiotic dosage; 0 ml kg-1 (control, 5 ml kg-1, 10 ml kg-1 and 15 ml kg-1 were tested in this study. The fish was fed twice a day at 08.00 AM and 06.00 PM at the ration of 5% body weight for 80 days. Results The results showed that growth performance and feed efficiency increased with increasing probiotic dosage in the diet from control (no probiotic to 10 ml kg-1 of probiotic dosage and then decreased when the dosage was increased up to 15 ml kg-1. Conclusions The best values for all measured parameters were recorded at the dosage of 10 ml kg-1. Therefore, it was concluded that the optimum dosage of enhanced probiotic for T. tambra fry was 10 ml kg-1 of feed.

  5. Oligomeric protein complexes of apolipoproteins stabilize the internal fluid environment of organism in redfins of the Tribolodon genus [Pisces; Cypriniformes, Cyprinidae].

    Science.gov (United States)

    Andreeva, Alla M; Serebryakova, Marina V; Lamash, Nina E

    2017-06-01

    One of the most important functions of plasma proteins in vertebrates is their participation in osmotic homeostasis in the organism. Modern concepts about plasma proteins and their capillary filtration are based on a model of large monomeric proteins that are able to penetrate the interstitial space. At the same time, it was revealed that a considerable amount of oligomeric complexes are present in the low-molecular-weight (LM) protein fraction in the extracellular fluids of fishes. The functions of these complexes are unknown. In the present study, we investigated the LM-fraction proteins in the plasma and interstitial fluid (IF) of redfins of the genus Tribolodon. This fish alternatively spends parts of its life cycle in saline and fresh waters. We identified the protein Wap65, serpins and apolipoproteins in this fraction. By combining the methods of 2D-E under native and denaturing conditions with MALDI, we demonstrated that only apolipoproteins formed complexes. We showed that serum apolipoproteins (АроА-I, Аро-14) were present in the form of homooligomeric complexes that were dissociated with the release of monomeric forms of proteins in the course of capillary filtration to IF. Dissociation of homooligomers is not directly correlated with the change in salinity but is correlated with seasonal dynamics. We found that there was a significant decrease in the total protein concentration in IF relative to plasma. Therefore, we suggested that dissociation of homooligomeric complexes from various apolipoproteins supports the isoosmoticity of extracellular fluids relative to capillary wall stabilization through a fluid medium in fish. Copyright © 2017 Elsevier Inc. All rights reserved.

  6. Gene mapping of 28S and 5S rDNA sites in chromosomes of two Barbus species and their F1 hybrids (Teleostei, Cyprinidae

    Directory of Open Access Journals (Sweden)

    Aneta Spoz

    2015-11-01

    Chromosomal location of ribosomal DNA sequences is useful for comparative cytogenetic fish studies due to their relatively fast rate of evolution. The results of species from the family Barbinae comparatively presented here for the first time, and they may support further taxonomical studies of the Barbus species.

  7. Molecular evolution of myoglobin in the Tibetan Plateau endemic schizothoracine fish (Cyprinidae, Teleostei) and tissue-specific expression changes under hypoxia.

    Science.gov (United States)

    Qi, Delin; Chao, Yan; Zhao, Yongli; Xia, Mingzhe; Wu, Rongrong

    2018-04-01

    Myoglobin (Mb) is an oxygen-binding hemoprotein that was once thought to be exclusively expressed in oxidative myocytes of skeletal and cardiac muscle where it serves in oxygen storage and facilitates intracellular oxygen diffusion. In this study, we cloned the coding sequence of the Mb gene from four species, representing three groups, of the schizothoracine fish endemic to the Qinghai-Tibetan Plateau (QTP), then conducted molecular evolution analyses. We also investigated tissue expression patterns of Mb and the expression response to moderate and severe hypoxia at the mRNA and protein levels in a representative of the highly specialized schizothoracine fish species, Schizopygopsis pylzovi. Molecular evolution analyses showed that Mb from the highly specialized schizothoracine fish have undergone positive selection and one positively selected residue (81L) was identified, which is located in the F helix, close to or in contact with the heme. We present tentative evidence that the Mb duplication event occurred in the ancestor of the schizothoracine and Cyprininae fish (common carp and goldfish), and that the Mb2 paralog was subsequently lost in the schizothoracine fish. In S. pylzovi, Mb mRNA is expressed in various tissues with the exception of the intestine and gill, but all such tissues, including the liver, muscle, kidney, brain, eye, and skin, expressed very low levels of Mb mRNA (Tibetan Plateau fish.

  8. Genetic variation of wild and hatchery populations of the catla Indian major carp (Catla catla Hamilton 1822: Cypriniformes, Cyprinidae revealed by RAPD markers

    Directory of Open Access Journals (Sweden)

    S.M. Zakiur Rahman

    2009-01-01

    Full Text Available Genetic variation is a key component for improving a stock through selective breeding programs. Randomly amplified polymorphic DNA (RAPD markers were used to assess genetic variation in three wild population of the catla carp (Catla catla Hamilton 1822 in the Halda, Jamuna and Padma rivers and one hatchery population in Bangladesh. Five decamer random primers were used to amplify RAPD markers from 30 fish from each population. Thirty of the 55 scorable bands were polymorphic, indicating some degree of genetic variation in all the populations. The proportion of polymorphic loci and gene diversity values reflected a relatively higher level of genetic variation in the Halda population. Sixteen of the 30 polymorphic loci showed a significant (p < 0.05, p < 0.01, p < 0.001 departure from homogeneity and the F ST values in the different populations indicated some degree of genetic differentiation in the population pairs. Estimated genetic distances between populations were directly correlated with geographical distances. The unweighted pair group method with averages (UPGMA dendrogram showed two clusters, the Halda population forming one cluster and the other populations the second cluster. Genetic variation of C. catla is a useful trait for developing a good management strategy for maintaining genetic quality of the species.

  9. Genotoxic effects and gene expression in Danio rerio (Hamilton 1822) (Cypriniformes: Cyprinidae) exposed to mining-impacted tributaries in Manizales, Colombia.

    Science.gov (United States)

    Ossa-López, Paula A; Castaño-Villa, Gabriel J; Rivera-Páez, Fredy A

    2017-09-25

    The zebrafish (Danio rerio) is one of the most studied aquatic organisms for water biomonitoring, due to its sensitivity to environmental degradation and resistance to toxic substances. This study determined the presence of micronuclei and nuclear abnormalities in peripheral blood erythrocytes, and assessed the gene expression of caspase-3 (CASP-3) and metallothionein 1 (MT-1) in the gills and liver of D. rerio. The study fish (n = 45) were exposed to water collected from two stations with mining impact (E2 and E3) and a reference station without evident mining contamination (E1), all located in La Elvira stream (Manizales-Colombia). In addition, a positive control (PC) with HgCl 2 (50 μg/L) and negative control (NC) with tap water were included. The fish from the PC and E2 and E3 treatments displayed genotoxic effects and changes in gene expression, with significant differences in micronuclei formation and the presence of blebbed nuclei. The cytochrome oxidase subunit I (COI) gene was used as reference and proved to be stable compared to the β-actin and 28S ribosomal RNA (28S) genes. In gills, CASP-3 expression was higher in the PC, and MT-1 expression was higher in the PC and E3 treatment. In liver, CASP-3 was expressed in the E2 treatment, and MT-1 expression was low. These results show that the genotoxic effects and differential gene expression observed in fish exposed to water from La Elvira stream could also be affecting the organisms present in this habitat.

  10. [Effect of food availability in early ontogenesis on the rate of growth and numbers of bream Abramis brama L. (Cypriniformes, Cyprinidae) in Kursh Bay of the Baltic Sea].

    Science.gov (United States)

    Naumenko, E N

    2011-01-01

    On the basis of studies of zooplankton and ichthyoplankton in the coastal zone of Kursh Bay of the Baltic Sea, zooplankton production accessible for juveniles of bream Abramis brama L. at early stages of ontogenesis, degree of removal of food resources, and food availability of juveniles of the bream that was reflected in the rate of growth were calculated. It is concluded that the removal of more than 60% of production of food zooplankton by bream juveniles leads to an increase of their mortality at early stages and a decrease in the rate of increase in the body weight.

  11. Contributions to the morphological variation of the common gudgeon, Gobio gobio complex (Teleostei: Cyprinidae), in the upper Vistula drainage (southeast Poland)

    Czech Academy of Sciences Publication Activity Database

    Nowak, M.; Mendel, Jan; Szczerbik, P.; Klaczak, A.; Mikolajczyk, T.; Ozga, K.; Falowska, B.; Popek, W.

    2011-01-01

    Roč. 19, č. 1 (2011), s. 37-49 ISSN 1230-6428 R&D Projects: GA ČR GP206/09/P608 Institutional research plan: CEZ:AV0Z60930519 Keywords : Gobioninae * traditional morphometrics * Vistula Subject RIV: EG - Zoology

  12. Effects of Temperature on Auditory Sensitivity in Eurythermal Fishes: Common Carp Cyprinus carpio (Family Cyprinidae) versus Wels Catfish Silurus glanis (Family Siluridae)

    Science.gov (United States)

    Maiditsch, Isabelle Pia; Ladich, Friedrich

    2014-01-01

    Background In ectothermal animals such as fish, -temperature affects physiological and metabolic processes. This includes sensory organs such as the auditory system. The reported effects of temperature on hearing in eurythermal otophysines are contradictory. We therefore investigated the effect on the auditory system in species representing two different orders. Methodology/Principal Findings Hearing sensitivity was determined using the auditory evoked potentials (AEP) recording technique. Auditory sensitivity and latency in response to clicks were measured in the common carp Cyprinus carpio (order Cypriniformes) and the Wels catfish Silurus glanis (order Siluriformes) after acclimating fish for at least three weeks to two different water temperatures (15°C, 25°C and again 15°C). Hearing sensitivity increased with temperature in both species. Best hearing was detected between 0.3 and 1 kHz at both temperatures. The maximum increase occurred at 0.8 kHz (7.8 dB) in C. carpio and at 0.5 kHz (10.3 dB) in S. glanis. The improvement differed between species and was in particular more pronounced in the catfish at 4 kHz. The latency in response to single clicks was measured from the onset of the sound stimulus to the most constant positive peak of the AEP. The latency decreased at the higher temperature in both species by 0.37 ms on average. Conclusions/Significance The current study shows that higher temperature improves hearing (lower thresholds, shorter latencies) in eurythermal species from different orders of otophysines. Differences in threshold shifts between eurythermal species seem to reflect differences in absolute sensitivity at higher frequencies and they furthermore indicate differences to stenothermal (tropical) species. PMID:25255456

  13. Feeding behavior of black carp Mylopharyngodon piceus (Pisces: Cyprinidae) on fry of other fish species and trematode transmitting snail species

    DEFF Research Database (Denmark)

    Hung, Nguyen Manh; The, Dang Tat; Stauffer, Jay R.

    2014-01-01

    Fish raised in aquaculture ponds may get infected with fishborne zoonotic trematodes (FZT) during the nursing stage. Freshwater snails serve as intermediate hosts for FZT and we wanted to explore the possibility of controlling snails by stocking nursery ponds with a few juvenile specimens...

  14. Geological events and Pliocene climate fluctuations explain the phylogeographical pattern of the cold water fish Rhynchocypris oxycephalus (Cypriniformes: Cyprinidae) in China.

    Science.gov (United States)

    Yu, Dan; Chen, Ming; Tang, Qiongying; Li, Xiaojuan; Liu, Huanzhang

    2014-10-25

    Rhynchocypris oxycephalus is a cold water fish with a wide geographic distribution including the relatively warm temperate regions of southern China. It also occurs in second- and third-step geomorphic areas in China. Previous studies have postulated that high-altitude populations of R. oxycephalus in southern China are Quaternary glacial relics. In this study, we used the mitochondrial gene Cytb and the nuclear gene RAG2 to investigate the species phylogeographical patterns and to test two biogeographic hypotheses: (1) that divergence between lineages supports the three-step model and (2) climatic fluctuations during the Quaternary resulted in the present distribution in southern China. Phylogenetic analysis detected three major matrilines (A, B, and C); with matrilines B and C being further subdivided into two submatrilines. Based on genetic distances and morphological differences, matriline A potentially represents a cryptic subspecies. The geographic division between matrilines B and C coincided with the division of the second and third geomorphic steps in China, suggesting a historical vicariance event. Pliocene climatic fluctuations might have facilitated the southwards dispersal of R. oxycephalus in matriline C, with the subsequent warming resulting in its split into submatrilines C1 and C2, leaving submatriline C2 as a relic in southern China. Our study demonstrates that geological events (three steps orogenesis) and climate fluctuations during the Pliocene were important factors in shaping phylogeographical patterns in R. oxycephalus. Notably, no genetic diversity was detected in several populations, all of which possessed unique genotypes. This indicates the uniqueness of local populations and calls for a special conservation plan for the whole species at the population level.

  15. A review of the Pseudobarbus afer (Peters, 1864 species complex (Teleostei, Cyprinidae in the eastern Cape Fold Ecoregion of South Africa

    Directory of Open Access Journals (Sweden)

    Albert Chakona

    2017-02-01

    Full Text Available The Eastern Cape redfin, Pseudobarbus afer, has long been considered to be a single widespread and variable species occurring in multiple isolated river systems in the Cape Fold Ecoregion (CFE at the southern tip of Africa. Mitochondrial cytochrome b and control region sequence data of individuals from populations currently assigned to P. afer across the species’ distribution range revealed existence of four deeply divergent taxonomic units: (i the Mandela lineage confined to the Sundays, Swartkops and Baakens river systems, (ii the Krom lineage endemic to the Krom River system, (iii the St Francis lineage occurring in the Gamtoos and adjacent river systems, and (iv the Forest lineage occurring in several coastal river systems from the Tsitsikamma to the Klein Brak River system. The Forest lineage is closely related to P. phlegethon from the Olifants River system on the west coast of South Africa, suggesting that it does not belong to P. afer s.l. Herein we focus on the three lineages within the P. afer s.l. complex and provide new diagnosis for P. afer s.s (Mandela lineage, revalidate P. senticeps (Krom lineage as a distinct species, and describe a new species P. swartzi (St Francis lineage. The three species exhibit subtle differences, which explains why they were previously considered to represent a single variable and widespread species. Pseudobarbus senticeps differs from both P. afer and P. swartzi by having fewer (i.e. larger scales (25–33, mode 29 lateral line scale series; 10–12, mode 11 circumpeduncular scales and presence of a lateral stripe which terminates in a conspicuous triangular blotch at the base of the caudal fin. Long barbels which reach or surpass the vertical through the posterior edge of the eye further separate P. senticeps from P. afer s.s. which possesses simple short barbels which do not reach the vertical through the posterior margin of the eye. Pseudobarbus afer s.s differs from P. swartzi sp. n. by possession of fewer scale rows along the lateral line (29–35, mode 32 vs 34–37, mode 36 in P. swartzi, fewer scales around the caudal peduncle (12–16, mode 12 vs 13–17, mode 16 in P. swartzi and a distinct mesh or net-like pigmentation pattern on latero-ventral scales.

  16. Distribuição espacial e temporal de Cetengraulis edentulus (Cuvier (Actinopterygii, Engraulidae na Baía de Sepetiba, Rio de Janeiro, Brasil Spatial and temporal distribution of Cetengraulis edentulus (Cuvier (Actinopterygii-Engraulidae in the Sepetiba Bay, Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Márcio de Araújo Silva

    2003-12-01

    Full Text Available Anchovies are members of the Engraulidae family, characterized to present coastal pelagic habits, concentrating in large schoolings distributed among the continental shelf and semi-closed environment, like bays, where they are target of heavy fisheries. The present study aims to describe spatial and temporal distribution of Cetengraulis edentulous (Cuvier, 1828 in the Sepetiba bay (22º54'-23º04'S, 43º34'-44º10'W and to assess influences of environmental variables on fish occurrence. A monthly sampling programme was carried out between October 1998 and September 1999, to take both, fish and environmental information on temperature, salinity and depth. Three bay zones were established based on spatial gradient of salinity and depth. Adults C. edentulus were more abundant in the inner bay zone; seasonally, larger size groups (total length > 16 cm occurred in spring/summer. Significant negative correlations were found between fish abundance and salinity and depth. The close relationship of this species to innermost areas in bays, and the absence of juveniles in sandy beaches, suggest that young-of-the-year use mangrove areas as recruitment sites.

  17. História natural dos amborés e peixes-macaco (Actinopterygii, Blennioidei, Gobioidei do Parque Nacional Marinho do Arquipélago de Fernando de Noronha, sob um enfoque comportamental Natural history focus blennies and gobis behaviour (Actinopterygii, Blennioidei, Gobioidei from Fernando de Noronha Archipelago

    Directory of Open Access Journals (Sweden)

    Liana de F. Mendes

    2006-09-01

    Full Text Available Os amborês e peixes-macaco, como são popularmente conhecidos gobiídeos e bleniídeos, são elementos dominantes da fauna de pequenos peixes bentônicos e litorâneos que habitam recifes tropicais, compondo grande parte da alta diversidade das espécies de pequeno porte encontrada no Atlântico ocidental. O estudo da distribuição e história natural dos gobióides e blenióides de Fernando de Noronha foi desenvolvido através de observações de campo. Além da descrição dos hábitats preferenciais foram abordados aspectos sociais tais como, habitat preferencial, interações intra- e interespecíficas, comportamento domiciliar e territorial, e comportamento reprodutivo. A maioria das espécies em foco apresenta distribuição principalmente litorânea, são gregárias, com áreas domiciliares relativamente pequenas em torno de 2 x 2 m², pouco agressivas, tendo sido ocasionalmente registradas curtas perseguições intra- e interespecíficas. A baixa agressividade registrada, em comparação com outros peixes é provavelmente associada à adaptação à ocupação de pequenas áreas, como é o caso das poças de marés, pois um grande número de interações agressivas representaria um gasto de energia desnecessário. No geral, as espécies possuem coloração críptica, associada à evitação de predadores. Apenas nestas épocas de reprodução foi observada a intensificação da agressividade, com comportamento territorial - defesa de território incluindo mordidas e perseguições.The gobis and blenis (Gobiidae and Bleniidae are the most important group of small benthic littoral fishes in tropical reefs, representing most of the high number of small fish species found in Western Atlantic. The natural history of gobiid and bleniid fish from Fernando de Noronha was assessed by means observation sessions using both snorkelling and scuba diving. Most of these observations were aimed at social behaviour, such as intra and interespecific interactions, territorial and reproductive behaviour. In general, these fish from Fernando de Noronha occurs in intertidal zone, are gregarious, with small home ranges of near 2 x 2 m², and show low agressivity towards other fish. Occasionally, some chasing was recorded between individuals of the same or distinct species. Low agressivity is probably associated to the occupation of small areas (e.g. tide pools, as a high degree of agressivity may represent an unnecessary [excessive] waste of energy. Most of the studied species presents cryptic coloration, associated to the avoidance of predators, but some males showed conspicuous coloration and intense aggressive behaviour, mainly during the reproductive season. It was only during this occasion that territorial behaviour, chasing and bites were recorded.

  18. Territorial hypothesis predicts the trade-off between reproductive opportunities and parental care in three species of damselfishes (Pomacentridae: Actinopterygii La hipótesis territorial predice la compensación entre las oportunidades reproductivas y el cuidado parental en tres especies de peces damisela (Pomacentridae: Actinopterygii

    Directory of Open Access Journals (Sweden)

    Eduardo Bessa

    2012-03-01

    Full Text Available Paternal care is rare in most animals, but common in fishes, including Pomacentridae. One way of explaining it is through the territorial hypothesis, which predicts that paternal care may evolve when caring for a territory with eggs will not cost more than territories without eggs. Here we describe the reproductive behavior of three pomacentrids from São Paulo, Brazil, and evaluate if the territorial hypothesis explains their behavioral variation through five predictions. We checked if females have multiple spawns (prediction 1, if they forage more (prediction 2 and if territory defence demands more when eggs are present (prediction 3. We also described how visible eggs are (prediction 4 and how available nesting sites are (prediction 5. Chromis multilineata differed from Abudefduf saxatilis and Stegastes fuscus by not guarding the eggs, spending less time with territoriality and fanning eggs; and more time feeding and foraging. Females of the three species are iteroparous. Females' A. saxatilis and S. fuscus foraged more than males, which spent the same amount of time in territorial defence before and after eggs were delivered. These two species also have very conspicuous egg clutches on clean rock surfaces defended by them. On the other hand, males and females of C. multilineata spent the same time foraging, while territoriality took more effort after eggs were present and it spawns cryptic eggs amid Sargassum, a common and disperse micro-environment in São Paulo rocky shores. Territorial hypothesis explains why paternal care by A. saxatilis and S. fuscus lasts longer than by C. multilineata.El cuidado paterno es raro en la mayoría de los animales, pero común en los peces, incluyendo los Pomacentridae. Una forma de explicar este fenómeno es la hipótesis territorial, que predice que el cuidado paterno puede evolucionar cuando la protección de un territorio con huevos no cuesta más que la de territorios sin huevos. Aquí se describe el comportamiento reproductivo de tres pomacentrídios de São Paulo, Brasil, y se evalúa si la hipótesis territorial explica la variación de su comportamiento a través de cinco predicciones. Se analizó si las hembras tienen múltiples desoves (predicción 1, si forrajean más (predicción 2 y si la defensa del territorio es mayor cuando los huevos están presentes (predicción 3. También se evaluó si los huevos eran visibles (predicción 4 y cuán disponibles eran los sitios de anidación (predicción 5. Chromis multilineata difería de Abudefduf saxatilis y Stegastes fuscus por no proteger a sus huevos, dedicar menos tiempo a la territorialidad y ventilación de sus huevos, y más tiempo para la alimentación y forrajeo. Las hembras de las tres especies son iteróparas. Las hembras de A. saxatilis y S. fuscus forrajean más que los machos, que gastan la misma cantidad de tiempo en la defensa territorial antes y después de liberar los huevos. Estas dos especies también defienden a desoves muy conspicuos adheridos en superficies de roca limpia. Por otra parte, las hembras y machos de C. multilineata pasaron el mismo tiempo forrajeando, mientras la territorialidad tomó más esfuerzo después, con los huevos presentes, y los desoves estaban escondidos en medio de Sargassum, un ambiente común y disperso en micro-ambientes de las costas rocosas de São Paulo. La hipótesis territorial explica el motivo por el cual el cuidado paterno de A. saxatilis y S. fuscus es más prolongado que el de C. multilineata.

  19. Environmental influences on distribution of four Sciaenidae species (Actinopterygii, Perciformes in a tropical bay at Southeastern Brazil Influências ambientais na distribuição de quatro espécies de Sciaenidae (Actinopterygii, Perciformes numa baía tropical do sudeste do Brasil

    Directory of Open Access Journals (Sweden)

    Francisco G. Araújo

    2006-06-01

    Full Text Available We sampled fish and measured environmental variables in the Sepetiba Bay, a tropical embayment at Southeastern Brazil. The aim was to assess environmental influences on distribution of four abundant species of Sciaenidae Micropogonias furnieri (Desmarest, 1823, Ctenosciaena gracilicirrhus (Metzelaar, 1919, Cynoscion leiarchus (Cuvier, 1830 and Menticirrhus americanus (Linnaeus, 1758 to test the habitat partition hypothesis. Samples were taken between June 1993 and July 1996, at three bay zones (outer, central and inner. Depth was the most important environmental variable to influence fish distribution, followed by transparency. M. furnieri showed significant negative correlation to depth, transparency and salinity, while C. gracilicirrhus showed the opposite situation. The highest densities of C. leiarchus occurred in the lowest temperature, while M. americanus did not showed preferences for any of the examined environmental variables. Spatial separation, mainly between M. furnieri and C. gracilicirrhus, seems to be the strategy developed to coexist in the Sepetiba Bay, with M. furnieri being more adapted to copy with harsh environmental conditions of the inner bay. The other three species, which use mostly the outer bay zone, seem to be restricted to more stable conditions, where influences from oceanic waters are more intense.Foram coletados peixes e medidas as variáveis ambientais na Baía de Sepetiba, uma baía tropical do Sudeste do Brasil. O objetivo foi determinar influências ambientais na distribuição de quatro espécies abundantes de Sciaenidae Micropogonias furnieri (Desmarest, 1823, Ctenosciaena gracilicirrhus (Metzelaar,1919, Cynoscion leiarchus (Cuvier,1830 and Menticirrhus americanus (Linnaeus, 1758 para testar a hipótese de partição do hábitat. Amostragens foram feitas entre Junho de 1993 e Julho de 1996 em três zonas da baía (externa, central e interna. A profundidade foi a mais importante variável ambiental que influenciou a distribuição dos peixes, seguida pela transparência. M. furnieri mostrou uma correlação negativa significante em relação à profundidade, transparência e salinidade, enquanto C. gracilicirrhus evidenciou uma situação oposta. As maiores densidades de C. leiarchus ocorreram nas menores temperaturas, enquanto M. americanus não parece ter preferências por nenhuma das variáveis ambientais examinadas. A separação espacial, principalmente entre M. furnieri e C. gracilicirrhus, parece ser uma estratégia desenvolvida para coexistirem na Baía de Sepetiba, com M. furnieri sendo mais adaptada para suportar as condições ambientais mais difíceis da zona interna. As outras três espécies, que utilizam mais a zona externa, parecem ser restritas às mais estáveis condições, onde influências das águas oceânicas são mais intensas.

  20. Nuevo registro de Pterygoplichthys disjunctivus (Actinopterygii: Loricariidae) en la desembocadura del río Coahuayana, Coahuayana, Michoacán, México New record of Pterygoplichthys disjunctivus (Actinopterygii: Loricariidae) in the mouth of Coahuayana River, Coahuayana, Michoacán, Mexico

    OpenAIRE

    Edgar Raúl Sandoval-Huerta; Xavier Madrigal-Guridi; Adrián García-Meraz; Néstor Irepani Dimas-Mora; Omar Domínguez-Domínguez

    2012-01-01

    En la desembocadura del río Coahuayana, al noroeste del estado de Michoacán fue registrada la presencia del plecostoma rayado, Pterygoplichthys disjunctivus (Loricariidae). Se describe e ilustra un ejemplar adulto de sexo indeterminado, con longitud total de 386 mm, recolectado el 4 de julio de 2010.Vermiculated Salifin Catfish Pterygoplichthys disjunctivus (Loricariidae), is reported at the mouth of the river Coahuayana, northwest of the state of Michoacán, Mexico. On July 4, 2010, an adult ...

  1. Nuevo registro de Pterygoplichthys disjunctivus (Actinopterygii: Loricariidae en la desembocadura del río Coahuayana, Coahuayana, Michoacán, México New record of Pterygoplichthys disjunctivus (Actinopterygii: Loricariidae in the mouth of Coahuayana River, Coahuayana, Michoacán, Mexico

    Directory of Open Access Journals (Sweden)

    Edgar Raúl Sandoval-Huerta

    2012-03-01

    Full Text Available En la desembocadura del río Coahuayana, al noroeste del estado de Michoacán fue registrada la presencia del plecostoma rayado, Pterygoplichthys disjunctivus (Loricariidae. Se describe e ilustra un ejemplar adulto de sexo indeterminado, con longitud total de 386 mm, recolectado el 4 de julio de 2010.Vermiculated Salifin Catfish Pterygoplichthys disjunctivus (Loricariidae, is reported at the mouth of the river Coahuayana, northwest of the state of Michoacán, Mexico. On July 4, 2010, an adult specimen of this species was collected. The specimen measured 386 mm and sex was not determined. The specimen is described and illustrated.

  2. The effect of ploidy and temporal changes in the biochemical profile of gibel carp (Carassius gibelio): a cyprinid fish species with dual reproductive strategies

    Czech Academy of Sciences Publication Activity Database

    Vetešník, Lukáš; Halačka, Karel; Šimková, A.

    2013-01-01

    Roč. 39, č. 2 (2013), s. 171-180 ISSN 0920-1742 R&D Projects: GA ČR GP524/09/P620; GA ČR GBP505/12/G112 Institutional support: RVO:68081766 Keywords : Biochemical profile of blood * Gibel carp * Ploidy * Temporal variability Subject RIV: EG - Zoology Impact factor: 1.676, year: 2013

  3. Two species of Rhabdochona (Nematoda: Rhabdochonidae) from the cyprinid fish Luciobarbus kersin (Heckel) in northern Iraq, including R. (Globochona) kurdistanensis sp. n

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Bilal, S. J.; Abdullah, S. M. A.

    2012-01-01

    Roč. 59, č. 2 (2012), s. 139-147 ISSN 0015-5683 R&D Projects: GA ČR GBP505/12/G112 Institutional research plan: CEZ:AV0Z60220518 Keywords : Parasitic nematode * Rhabdochona * freshwater fish * Luciobarbus * Iraq Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.515, year: 2012 http://folia.paru.cas.cz/pdfs/showpdf.php?pdf=22051

  4. Sequence structure and intragenomic variability of ribosomal ITS2 in monozoic tapeworms of the genus Khawia (Cestoda: Caryophyllidea), parasites of cyprinid fish

    Czech Academy of Sciences Publication Activity Database

    Kraľová-Hromadová, I.; Bazsalovicsová, E.; Oros, M.; Scholz, Tomáš

    2012-01-01

    Roč. 111, č. 4 (2012), s. 1621-1627 ISSN 0932-0113 R&D Projects: GA ČR GA524/08/0885; GA ČR GD206/09/H026; GA ČR GBP505/12/G112 Institutional research plan: CEZ:AV0Z60220518 Keywords : INTERNAL TRANSCRIBED SPACER-1 * ATRACTOLYTOCESTUS-HURONENSIS * SYMPATRIC SPECIATION * SINENSIS HSU * RDNA * DNA * PLATYHELMINTHES * PARALOGUES * EVOLUTION * carp Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.852, year: 2012 http://link.springer.com/article/10.1007%2Fs00436-012-3001-z

  5. Validation of a serum neutralization test for detection of antibodies specific to cyprinid herpesvirus 3 in infected common and koi carp (Cyprinus carpio)

    DEFF Research Database (Denmark)

    Cabon, J.; Louboutin, L.; Castric, J.

    2017-01-01

    healthy and naturally or experimentally CyHV-3-infected carp. French CyHV-3 isolate 07/108b was neutralized efficiently by sera from carp infected with European, American and Taiwanese CyHV-3 isolates, but no neutralization was observed using sera specific to other aquatic herpesviruses. Diagnostic...

  6. Chromosome studies of european cyprinid fishes: Cross-species painting reveals natural allotetraploid origin of a carassius female with 206 chromosomes

    Czech Academy of Sciences Publication Activity Database

    Knytl, M.; Kalous, L.; Symonová, Radka; Rylková, K.; Ráb, Petr

    2013-01-01

    Roč. 139, č. 4 (2013), s. 276-283 ISSN 1424-8581 R&D Projects: GA ČR(CZ) GPP506/11/P596 Institutional support: RVO:67985904 Keywords : fish cytogenetics * genome addition * GISH Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.905, year: 2013

  7. Bead-probe complex capture a couple of SINE and LINE family from genomes of two closely related species of East Asian cyprinid directly using magnetic separation

    Science.gov (United States)

    Tong, Chaobo; Guo, Baocheng; He, Shunping

    2009-01-01

    Background Short and long interspersed elements (SINEs and LINEs, respectively), two types of retroposons, are active in shaping the architecture of genomes and powerful tools for studies of phylogeny and population biology. Here we developed special protocol to apply biotin-streptavidin bead system into isolation of interspersed repeated sequences rapidly and efficiently, in which SINEs and LINEs were captured directly from digested genomic DNA by hybridization to bead-probe complex in solution instead of traditional strategy including genomic library construction and screening. Results A new couple of SINEs and LINEs that shared an almost identical 3'tail was isolated and characterized in silver carp and bighead carp of two closely related species. These SINEs (34 members), designated HAmo SINE family, were little divergent in sequence and flanked by obvious TSD indicated that HAmo SINE was very young family. The copy numbers of this family was estimated to 2 × 105 and 1.7 × 105 per haploid genome by Real-Time qPCR, respectively. The LINEs, identified as the homologs of LINE2 in other fishes, had a conserved primary sequence and secondary structures of the 3'tail region that was almost identical to that of HAmo SINE. These evidences suggest that HAmo SINEs are active and amplified recently utilizing the enzymatic machinery for retroposition of HAmoL2 through the recognition of higher-order structures of the conserved 42-tail region. We analyzed the possible structures of HAmo SINE that lead to successful amplification in genome and then deduced that HAmo SINE, SmaI SINE and FokI SINE that were similar in sequence each other, were probably generated independently and created by LINE family within the same lineage of a LINE phylogeny in the genomes of different hosts. Conclusion The presented results show the advantage of the novel method for retroposons isolation and a pair of young SINE family and its partner LINE family in two carp fishes, which strengthened the hypotheses containing the slippage model for initiation of reverse transcription, retropositional parasitism of SINEs on LINEs, the formation of the stem loop structure in 3'tail region of some SINEs and LINEs and the mechanism of template switching in generating new SINE family. PMID:19224649

  8. Bead-probe complex capture a couple of SINE and LINE family from genomes of two closely related species of East Asian cyprinid directly using magnetic separation

    Directory of Open Access Journals (Sweden)

    Guo Baocheng

    2009-02-01

    Full Text Available Abstract Background Short and long interspersed elements (SINEs and LINEs, respectively, two types of retroposons, are active in shaping the architecture of genomes and powerful tools for studies of phylogeny and population biology. Here we developed special protocol to apply biotin-streptavidin bead system into isolation of interspersed repeated sequences rapidly and efficiently, in which SINEs and LINEs were captured directly from digested genomic DNA by hybridization to bead-probe complex in solution instead of traditional strategy including genomic library construction and screening. Results A new couple of SINEs and LINEs that shared an almost identical 3'tail was isolated and characterized in silver carp and bighead carp of two closely related species. These SINEs (34 members, designated HAmo SINE family, were little divergent in sequence and flanked by obvious TSD indicated that HAmo SINE was very young family. The copy numbers of this family was estimated to 2 × 105 and 1.7 × 105 per haploid genome by Real-Time qPCR, respectively. The LINEs, identified as the homologs of LINE2 in other fishes, had a conserved primary sequence and secondary structures of the 3'tail region that was almost identical to that of HAmo SINE. These evidences suggest that HAmo SINEs are active and amplified recently utilizing the enzymatic machinery for retroposition of HAmoL2 through the recognition of higher-order structures of the conserved 42-tail region. We analyzed the possible structures of HAmo SINE that lead to successful amplification in genome and then deduced that HAmo SINE, SmaI SINE and FokI SINE that were similar in sequence each other, were probably generated independently and created by LINE family within the same lineage of a LINE phylogeny in the genomes of different hosts. Conclusion The presented results show the advantage of the novel method for retroposons isolation and a pair of young SINE family and its partner LINE family in two carp fishes, which strengthened the hypotheses containing the slippage model for initiation of reverse transcription, retropositional parasitism of SINEs on LINEs, the formation of the stem loop structure in 3'tail region of some SINEs and LINEs and the mechanism of template switching in generating new SINE family.

  9. Coexistence of fish species in a large lowland river: food niche partitioning between small-sized percids, cyprinids and sticklebacks in submersed macrophytes.

    Science.gov (United States)

    Dukowska, Małgorzata; Grzybkowska, Maria

    2014-01-01

    In the spring and summer of each year, large patches of submersed aquatic macrophytes overgrow the bottom of the alluvial Warta River downstream of a large dam reservoir owing to water management practices. Environmental variables, macroinvertebrates (zoobenthos and epiphytic fauna, zooplankton) and fish abundance and biomass were assessed at this biologically productive habitat to learn intraseasonal dynamics of food types, and their occurrence in the gut contents of small-sized roach, dace, perch, ruffe and three-spined stickleback. Gut fullness coefficient, niche breadth and niche overlap indicated how the fishes coexist in the macrophytes. Chironomidae dominated in the diet of the percids. However, ruffe consumed mostly benthic chironomids, while perch epiphytic chironomids and zooplankton. The diet of dace resembled that in fast flowing water although this rheophilic species occurred at unusual density there. The generalist roach displayed the lowest gut fullness coefficient values and widest niche breadth; consequently, intraspecific rather than interspecific competition decided the fate of roach. Three-spined stickleback differed from the other fishes by consuming epiphytic simuliids and fish eggs. The diet overlap between fishes reaching higher gut fullness coefficient values was rather low when the food associated with the submersed aquatic macrophytes was most abundant; this is congruent with the niche overlap hypothesis that maximal tolerable niche overlap can be higher in less intensely competitive conditions.

  10. Summer co-existence of small-sized cyprinid and percid individuals in natural and impounded stretches of a lowland river: food niche partitioning among fishes.

    Science.gov (United States)

    Lik, J; Dukowska, M; Grzybkowska, M; Leszczyńska, J

    2017-04-01

    Due to changes of discharge regime downstream of a dam reservoir, an alluvial natural stretch of the Warta River changed to a macrophyte-dominated ecosystem. Large patches of submersed, aquatic macrophytes appeared in summer and their effect is analysed in this study. These patches contained enriched macroinvertebrate assemblages (epiphyton and benthos) and they were refuge for both zooplankton and young fishes released from the reservoir. Despite these altered conditions in this stretch, roach Rutilus rutilus, perch Perca fluviatilis and ruffe Gymnocephalus cernua dominated, as they did in the natural backwater. Fishes were sampled every 2 weeks from June to August, together with their food resources to assess the partitioning of the diet among small individuals of the three species in both stretches (the natural and affected ones). The aim of the analysis was to answer how animal food associated with water plants was partitioned between the species. In both stretches, G. cernua were primarily benthivorous, but epiphytic fauna, zooplankton and large-sized benthic chironomid larvae replaced lack of many large, benthic insects in the tailwater. Levins' food breath index decreased from 0·36 in the backwater to 0·29 in the tailwater. An opposite trend was observed for P. fluviatilis occurring among macrophytes. Perca fluviatilis were competitors of R. rutilus and took food not only in or on the river bed, but also in the water column. They ate zooplankton and epiphytic fauna and Levins' index increased from 0·32 to 0·44 in the tailwater. Rutilus rutilus fed on adult insects, algae and plant fragments in the natural stretch. In the tailwater, these food types were chiefly complemented by zooplankton. Despite this, the niche breadth of R. rutilus was similar at the two sites. Abundance of food associated with the macrophytes appeared to facilitate cohabitation in the abundant fish populations, but P. fluviatilis appeared to benefit the most in the altered river stretch. © 2017 The Fisheries Society of the British Isles.

  11. Virulence of a chimeric recombinant infectious haematopoietic necrosis virus expressing the spring viraemia of carp virus glycoprotein in salmonid and cyprinid fish

    Science.gov (United States)

    Emmenegger, Eveline; Biacchesi, Stéphane; Mérour, Emilie; Glenn, Jolene. A; Palmer, Alexander D.; Brémont, Michel; Kurath, Gael

    2018-01-01

    Infectious haematopoietic necrosis virus (IHNV) and spring viraemia of carp virus (SVCV) are both rhabdoviruses of fish, listed as notifiable disease agents by the World Organization for Animal Health. Recombinant rhabdoviruses with heterologous gene substitutions have been engineered to study genetic determinants and assess the potential of these recombinant viruses for vaccine development. A recombinant IHNV (rIHNV), containing the full-length genome of a European IHNV strain, was modified by deleting the glycoprotein (G) gene and replacing it with a European SVCV G-gene to make the rIHNV-Gsvcv. The chimeric rIHNV-Gsvcv level of virulence in rainbow trout, common carp and koi was assessed, and its ability to induce a protective immune response in surviving koi against wild-type SVCV infection was tested. The rIHNV-Gsvcv infection of trout led to high mortality, ranging from 78% to 92.5%, after immersion. In contrast, no deaths occurred in juvenile common carp after infection with rIHNV-Gsvcv by either immersion or intraperitoneal (IP) injection. Similarly, koi infected with rIHNV-Gsvcv via IP injection had little to no mortality (≤9%). Koi that survived initial infection with a high dose of recombinant virus rIHNV-Gsvcv were protected against a virulent SVCV challenge resulting in a high relative per cent survival of 82.5%.

  12. Effects of metal ions on cyprinid fish immune response: In vitro effects of Zn2+ and Mn2+ on the mitogenic response of carp pronephros lymphocytes

    International Nuclear Information System (INIS)

    Ghanmi, Z.; Rouabhia, M.; Othmane, O.; Deschaux, P.A.

    1989-01-01

    Lymphocytes from the pronephros of carp (Cyprinus carpio L) have been subjected to transformation by mitogens, concanavalin A (Con A), phytohemagglutinin (PHA), and lipopolysaccharides (LPS), with Zn or Mn at varying concentrations. Addition of Zn 2+ (10(-7) to 10(-3) M) to mitogen-stimulated T and B cells enhanced [ 3 H]thymidine incorporation. Addition of 10(-5) M Zn 2+ inhibited the response to Con A, PHA, and LPS. At this concentration, Zn was toxic. Addition of Mn2+ (10(-7) to 10(-3) M) to mitogen-stimulated lymphocytes enhanced [ 3 H]thymidine incorporation. This effect was observed with Con A- and PHA-stimulated lymphocytes, but not with LPS-stimulated lymphocytes. In contrast, addition of 10(-1) M Mn 2+ to lymphocyte cultures exerted an inhibitor on the response to Con A or to PHA, while the response to LPS was unaffected. Addition of 10(-1) M Mn 2+ to Con A- or PHA-stimulated cultures at different times after initiation of stimulation indicated that Mn 2+ was inhibitory only when it was added before the first 16 hr of cultures. The inhibition induced by 10(-1) M Mn2+ could be reversed by adding 2 mM CaCl 2 to the culture

  13. a survey of the artisanal fisheries of kontagora reservoir, niger state

    African Journals Online (AJOL)

    DR. AMINU

    , Nigeria. Fish families /Species. English. Local Name. Cichlidae. Tilapia melanopleura. Tilapia. Gargaza. Hemichromis fasciatus. Tiliapia. Kulkula. Cyprinidae. Barilius niloticus. Barb. Dankursa. Bagridae. Auchenoglanis occidentalis. Cat fish.

  14. Morphological and quantitative analysis of myenteric plexus neurons of intestinal bulb of Cyprinus carpio (Linnaeus, 1758 (Osteichthyes, Cyprinidae Análise morfológica e quantitativa dos neurônios do plexo mientérico do bulbo intestinal de Cyprinus Carpio. (Linnaeus, 1758 (Osteichthyes, Cyprinidae

    Directory of Open Access Journals (Sweden)

    Patrícia Marega

    2000-05-01

    Full Text Available The myenteric plexus shows morphologic and quantitative variability in the alimentary canals of different species of animals. We have quantified and analyzed the morphology of the myenteric plexus of ten adult Cyprinus carpio intestinal bulbs, by means of histological cross sections stained with HE and Van Gieson methods, as well as Giemsa-stained whole mount preparations. The myenteric plexus, located between the longitudinal and circular layers of the muscular tunic, is formed by isolated neurons and ganglia. Percentages of small, medium and large neurons were 21%, 63.4% and 15.6%, respectively, with a prevalence of intermediary cytoplasmic basophily and peripheral nuclei. Neuronal density in 6.92mm2 of intestinal bulb was of 2,040 neuronsO plexo mientérico apresenta variabilidade morfológica e quantitativa ao longo do tubo digestório das diferentes espécies animais. No presente trabalho quantificamos e analisamos a morfologia dos neurônios do plexo mientérico de dez bulbos intestinais de Cyprinus carpio, por meio de cortes histológicos corados com HE e Van Gieson e de preparados de membrana corados pelo método de Giemsa. O plexo mientérico foi localizado entre os estratos longitudinal e circular da túnica muscular, sendo constituído por neurônios isolados e por gânglios. As porcentagens de neurônios pequenos, médios e grandes foram 21%, 63,4% e 15,6%, respectivamente, predominando neurônios com basofilia citoplasmática intermediária e núcleo em posição periférica. A análise quantitativa revelou a presença de 2.040 neurônios/6,92mm2 de bulbo intestinal

  15. Aspects of the feeding and population structure of Leporinus piau Fowler, 1941 (Actinopterygii, Characiformes, Anostomidae of Taperoá II Dam, semi arid region of Paraíba, Brazil

    Directory of Open Access Journals (Sweden)

    Ana Karla Araujo Montenegro

    2010-06-01

    Full Text Available The aim of this research was to determine the population structure, weight-length relationship and sexual ratio of Leporinus piau. It also attempted to evaluate the relationship between the alimentary activity and the reproductive period, and to identify the diet and alimentary regimen. Six collections were made in the months of October and December of 2005 and March, June, August and October of 2006, using casting, seining and gill nets. Some specimens were sent to the UFPB Ichthyological Collection. The 89 collected individuals presented lengths varying from 6.2cm to 22cm. The growth of the species was of the negative allometric type. There was a significant predominance of males. The alimentary diet of L. piau was apparently variable, suggesting omnivorousness.

  16. Population and feeding structure of Steindachnerina notonota Miranda-Ribeiro, 1937 (Actinopterygii, Characiformes, Curimatidae in Taperoá II dam, semi-arid region of Paraíba, Brazil Estrutura populacional e alimentar de Steindachnerina notonota Miranda Ribeiro, 1937 (Actinopterygii, Characiformes, Curimatidae no Açude Taperoá II, na região do semi-árido da Paraíba, Brasil

    Directory of Open Access Journals (Sweden)

    Ana Karla Araujo Montenegro

    2011-09-01

    Full Text Available AIM: The present study investigated population structure, weight-length relationships, sex ratio and associations between feeding activity and reproductive period, diet and alimentary regime of Steindachnerina notonota at Taperoá II dam, semi-arid region of Paraíba State. METHODS: The region has the lowest pluviometric rates of Brazil. Fishes were collected during six sampling periods (October and December/2005, and March, June, August and October of 2006 encompassing two dry periods, and one rainy period. Cast, seine and gill nets were used to collect the individuals. RESULTS: Throughout the study period 120 individuals were collected, with total length ranging between 2.2 and 10.7 cm and weight between 0.31 and 43.1 g. A recruitment period, identified due to a higher abundance of small individuals in the population, was observed in the beginning of the rainy period of 2006, lasting until the beginning of the dry season. S. notonota showed a negative allometric growth type, i.e., individuals 'elongate' during their development. A significantly higher proportion of females were observed throughout the study period. The relationship between feeding and reproductive activities were not significant, given that individuals showed high feeding rates regardless of the intensity of their reproductive activities. Although the diet of S. notonota comprised several items such as plant debris, eggs, organic matter, phytoplankton, zooplankton, Nematoda, Ostracada, Conchostraca, among others, the most important feeding item was detritus, suggesting a predominant detritivirous feeding habit. CONCLUSIONS: Biological aspects of S. notonota observed in the present study suggest that this species has potential to attain great population sizes, as consequence of both diet and feeding dynamics, as well as its reproductive characteristics and growth type.OBJETIVO: O presente estudo investigou a estrutura da população, a relação peso-comprimento, a razão sexual, as associações entre atividade alimentar e período de reprodução, dieta e regime alimentar de Steindachnerina notonota, no açude Taperoá II, semi-árido paraibano. MÉTODOS: A região tem os menores índices pluviométricos do Brasil. Os peixes foram coletados em seis amostragens (Outubro e Dezembro de 2005, e Março, Junho, Agosto e Outubro de 2006, compreendendo 2 períodos de estiagem e 1 chuvoso. Para a captura dos exemplares, utilizou-se tarrafa, redes de espera e de arrasto. RESULTADOS: Ao longo do estudo capturou-se um total de 120 indivíduos, que mediram entre 2,2 e 10,7 cm de comprimento total e pesaram de 0,31 a 43,1 g. Evidenciou‑se intenso recrutamento durante todo o período estudado, demonstrado pela maior abundância de indivíduos em estádios gonadais “em maturação” (2 e “maduros (3”. S. notonota apresentou um crescimento do tipo alométrico negativo (quando os indivíduos se tornam mais “alongados” durante o seu desenvolvimento, com uma proporção sexual significativamente maior de fêmeas ao longo do período estudado. Observou-se que a relação entre a atividade alimentar e a atividade reprodutiva não foi significativa, tendo em vista que os indivíduos apresentaram alta atividade alimentar independente da intensidade da atividade reprodutiva. Embora a dieta alimentar de S. notonota tenha sido composta por diversos itens, a exemplo de restos vegetais, ovos, matéria orgânica, fitoplâncton, zooplâncton, Nematoda, Ostracoda, Conchostraca, dentre outros, o item de maior importância alimentar foi detritos, indicando um regime alimentar predominantemente detritívoro. CONCLUSÕES: Os aspectos da biologia de S. notonota observados no presente estudo, remetem a uma espécie com potencial para atingir grandes tamanhos populacionais, tanto pela sua dieta e dinâmica alimentar, quanto pelas suas características reprodutivas, e tipo de crescimento.

  17. Effects of parental number and duration of the breeding period on the effective population size and genetic diversity of a captive population of the endangered Tokyo bitterling Tanakia tanago (Teleostei: Cyprinidae).

    Science.gov (United States)

    Kubota, Hitoshi; Watanabe, Katsutoshi

    2012-01-01

    The maintenance of genetic diversity is one of the chief concerns in the captive breeding of endangered species. Using microsatellite and mtDNA markers, we examined the effects of two key variables (parental number and duration of breeding period) on effective population size (N(e) ) and genetic diversity of offspring in an experimental breeding program for the endangered Tokyo bitterling, Tanakia tanago. Average heterozygosity and number of alleles of offspring estimated from microsatellite data increased with parental number in a breeding aquarium, and exhibited higher values for a long breeding period treatment (9 weeks) compared with a short breeding period (3 weeks). Haplotype diversity in mtDNA of offspring decreased with the reduction in parental number, and this tendency was greater for the short breeding period treatment. Genetic estimates of N(e) obtained with two single-sample estimation methods were consistently higher for the long breeding period treatment with the same number of parental fish. Average N(e) /N ratios were ranged from 0.5 to 1.4, and were high especially in the long breeding period with small and medium parental number treatments. Our results suggest that the spawning intervals of females and alternative mating behaviors of males influence the effective size and genetic diversity of offspring in bitterling. To maintain the genetic diversity of captive T. tanago, we recommend that captive breeding programs should be conducted for a sufficiently long period with an optimal level of parental density, as well as using an adequate number of parents. © 2011 Wiley Periodicals, Inc.

  18. Molecular systematics and DNA barcoding of Altai osmans, oreoleuciscus (pisces, cyprinidae, and leuciscinae), and their nearest relatives, inferred from sequences of cytochrome b (Cyt-b), cytochrome oxidase c (Co-1), and complete mitochondrial genome.

    Science.gov (United States)

    Kartavtsev, Yuri Phedorovich; Batischeva, Natalia M; Bogutskaya, Nina G; Katugina, Anna O; Hanzawa, Naoto

    2017-07-01

    Mitochondrial DNA (mtDNA) at the protein-coding Cyt-b gene along with data retrieved from GenBank for Co-1 gene fragments and complete mitochondrial genome (mitogenome) of Altai osmans and the nearest relatives of Leuciscinae fish species were compared for the estimation of variability and phylogenetic tree building. Phylogenetic trees were built by four techniques: Bayesian (BA), maximum likelihood (ML), maximum parsimony (MP), and neighbor-joining (NJ). Resolution of Cyt-b trees for species of two genera (Oreoleuciscus and Phoxinus) was quite distinct at all the approaches. For Tribolodon, the single gene trees were not well resolved; however, the mitogenome tree was resolved. Species identification on per individual basis (DNA barcoding) was high for both Cyt-b and Co-1 genes. The trees built using the data for 13 protein mitochondrial genes revealed a complicated phylogenetic pattern within the subfamily Leuciscinae. Scores of the average p-distances at three taxonomic levels were considerably different: (1) 1.16 ± 0.96, (2) 8.21 ± 1.01, and (3) 16.41 ± 0.85 for Cyt-b and (1) 1.04 ± 0.78, (2) 8.30 ± 0.92, and (3) 10.74 ± 0.79 for 13 protein genes of mitogenome, where (1) is intraspecies, (2) is intragenus, and (3) is intrasubfamily levels. Data on mitogenome distances were summarized for the taxonomic hierarchy for the first time. A concordant increase in distance score with growth of the rank of taxa (having the minimum score at the intraspecies level), both for a single gene and the whole mitogenome, substantiates the concept that speciation in the subfamily Leuciscinae in most cases follows the geographic mode. The distinct clustering of Altai osmans, Oreoleuciscus potanini and O. humilis, in the Cyt-b and Co-1 gene trees with small overall genetic distances, obtained for both genes, allows us to consider these taxa as separate but genetically sister species.

  19. Introduction to Some Species of Argulus (Crustacea: Branchiura ...

    African Journals Online (AJOL)

    PROF HORSFALL

    2017-12-12

    Dec 12, 2017 ... has been found in Asia, Europe, Africa, Australia and. North, Central, and South .... of Culture Cyprinidae in Pen Culture of Anzali. Lagoon. .... PHM; Wendelaar Bonga, SE (1999). Modulation ... auratus auratus) in India. Biolife.

  20. New evidence of homoplasy within the African genus Varicorhinus ...

    African Journals Online (AJOL)

    New evidence of homoplasy within the African genus Varicorhinus (Cyprinidae): an independent origin of specialized scraping forms in the adjacent drainage systems of Ethiopia inferred from mtDNA analysis.

  1. An emergent multiple predator effect may enhance biotic resistance in a stream fish assemblage

    Science.gov (United States)

    Bret C. Harvey; Jason L. White; Rodney J. Nakamoto

    2004-01-01

    While two cyprinid fishes introduced from nearby drainages have become widespread and abundant in the Eel River of northwestern California, a third nonindigenous cyprinid has remained largely confined to <25 km of one major tributary (the Van Duzen River) for at least 15 years. The downstream limit of this species, speckled dace, does not appear to correspond...

  2. A digest of bony fish tapeworms

    Czech Academy of Sciences Publication Activity Database

    Scholz, Tomáš; Kuchta, Roman

    2017-01-01

    Roč. 67, č. 2 (2017), s. 43-58 ISSN 0240-8759 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Cestoda * review * diversity * host associations * distributions * phylogeny * Actinopterygii * biogeography Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 0.343, year: 2016

  3. Evaluating stocking of YOY pike Esox lucius as a tool in the restoration of shallow lakes

    DEFF Research Database (Denmark)

    Skov, Christian; Nilsson, P.A.

    2007-01-01

    from field surveys in eight study lakes stocked with YOY pike as well as from the literature. 3. Our model showed that all parameters studied were important for predicting the effects of pike stocking on cyprinids. In particular, body size at stocking, cyprinid production and pike survival were good...... as an appropriate and reliable tool for restoration programmes in shallow lakes, and indicate that when used efforts should be made to optimize the timing of stocking in relation to YOY cyprinid production and to increase the stocking body size of the pike.......1. Stocking of piscivores in shallow, eutrophicated lakes to reduce cyprinid densities is a common approach in lake restorations. Young-of-the-year (YOY) pike Esox lucius are frequently used to reduce cyprinid densities, but their effectiveness is equivocal. This study uses a simple model to assess...

  4. Age at maturity of Mediterranean marine fishes

    Directory of Open Access Journals (Sweden)

    A. C. TSIKLIRAS

    2014-07-01

    Full Text Available In this review we collected data on the age at maturity (tm and maximum reported age (tmax for 235 stocks of Mediterranean marine fishes, belonging to 82 species, 37 families, 12 orders and 2 classes (Actinopterygii and Elasmobranchii. Among Actinopterygii (mean tm ± SD = 2.20 ± 1.43 y, n = 215, tm ranged from 0.3 y, for the common goby Pomatoschistus microps, to 12 y, for dusky grouper Epinephelus marginatus, while among Elasmobranchii (mean tm ± SD = 5.94 ± 2.47 y, n = 20, tm ranged between 2.7 y, for brown ray Raja miraletus, and 12 y for picked dogfish Squalus acanthias. Overall, the tmax ranged between 1 y, for transparent goby Aphia minuta, and 70 y, for wreckfish Polyprion americanus. The mean tmax of Actinopterygii (tmax ± SD = 10.14 ± 9.42 y was lower than that of Elasmobranchii (tmax ± SD = 14.05 ± 8.47 y. The tm exhibited a strong positive linear relation with tmax for both Actinopterygii (logtm = 0.58 ´ logtmax – 0.25, r2 = 0.51, P < 0.001 and Elasmobranchii (logtm = 0.67 ´ logtmax – 0.006, r2 = 0.51, P = 0.007. The mean tm/tmax did not differ significantly with sex within Actinopterygii (ANOVA: F = 0.27, P = 0.60, n = 90; females: mean ± SD = 0.276 ± 0.143; males: mean ± SD = 0.265 ± 0.138 and Elasmobranchii (ANOVA: F = 1.44, P = 0.25, n = 10; females: mean ± SD = 0.499 ± 0.166; males: mean ± SD = 0.418 ± 0.133. Finally, the dimensionless ratio tm/tmax was significantly lower (ANOVA: F = 31.04, P < 0.001 for Actinopterygii (mean ± SD = 0.270 ± 0.135, n = 180 than for Elasmobranchii, (mean ± SD = 0.458 ± 0.152, n = 20, when stocks with combined sexes were excluded from the analysis.

  5. Células sangüíneas, eletrólitos séricos, relação hepato e esplenossomática de carpa-comum, Cyprinus carpio (Cyprinidae na primeira maturação gonadal - DOI: 10.4025/actascibiolsci.v26i1.1661 Blood cells, seric electrolyte and Cyprinus carpio (Cyprinidae common carp hepatosomatic and splenosomatic relation in the first gonadal maturation - DOI: 10.4025/actascibiolsci.v26i1.1661

    Directory of Open Access Journals (Sweden)

    Eugênio de Campos Filho

    2004-04-01

    Full Text Available Foram estudados em ambos os sexos de Cyprinus carpio o fator relativo de condição (Kn, a relação hepato (RHS e esplenossomática (RES, o eritrograma, trombograma, leucograma, a localização de glicogênio pelo método de PAS em trombócitos e leucócitos e os níveis séricos de proteína total e de eletrólitos. O Kn nas fêmeas foi maior que nos machos. Os valores médios da RES nas fêmeas foram inferiores aos dos machos, enquanto que o número de leucócitos, linfócitos e neutrófilos foram superiores; porém os níveis séricos de magnésio e de cloreto nos machos foram maiores quando comparados aos das fêmeas. Os valores médios da RHS, hematócrito, concentração de hemoglobina, volume corpuscular médio (VCM, concentração da hemoglobina corpuscular média (CHCM, número de eritrócitos, trombócitos, monócitos, eosinófilos e leucócitos granular PAS-positivos (LG-PAS, níveis séricos de proteína total, sódio, potássio e cálcio não evidenciaram diferença estatisticamente significativa (P>0,05, entre machos e fêmeas. O método de PAS mostra grânulos de glicogênio no citoplasma de trombócitos, eosinófilos e neutrófilos. Entretanto, no citoplasma dos LG-PAS foram detectados grânulos de glicoproteínas neutras. Monócitos, linfócitos foram PAS negativos. Nos teleósteos o amadurecimento das gônadas apresenta forte relação com o fator de condição. Na fase de reprodução nem sempre é possível detectar os efeitos do sexo isoladamente no hemograma, pois muitas vezes esse pode não ser o único fator ocasionando dimorfismoThis research studied the condition (Kn relative factor, the hepatosomatic (HSR, the splenosomatic (SSR relation, the erythrocytes, thrombocytes and leukocytes number, the glicogen locality by PAS method in thrombocytes and leucocytes and the total protein and electrolytes serices levels in both Cyprinus carpio L. sex. The female Kn showed higher value than the males. The female HSR average values were lower than the ones observed in males, while the leukocytes, lymphocytes and neutrophils percentage number were the highest. However, the males magnesium and choride serices levels were higher than the females. The HRS average values, hematocrit, haemoglobin concentration, average corspucular volume (ACV, average corpuscular haemoglobin concentration (ACHC, erythocytes number, trombocytes, monocytes, oesinophils, positive PAS leukocyte granular (PAS-LG, and total protein, sodium, potassium and calcium serices levels did not show significant statistics difference (p> 0.05 between males and females. The PAS method showed glicogen granules into the thronbocytes, eosinophils and neutrophils cytoplasm. Nevertheless, in the PAS-LG cytoplasm, neutral glicoprotein granules were also observed. Monocytes and lynfocytes showed negative reaction to PAS. In the teleosts, the gonodas matureness presents higher relation with the factor of condition. During the reprodution period it is unlikely to find the sexual effects isolated in the hemogram, because most of the time that is not the only factor to cause dimorphism

  6. Inter- and size-specific patterns of fish seasonal migration between a shallow lake and its streams

    DEFF Research Database (Denmark)

    Skov, Christian; Brodersen, J.; Nilsson, P.A.

    2008-01-01

    This study used passive telemetry (passive integrated transponders) to evaluate winter migration in three species of cyprinids (roach (Rutilus rutilus (L.)), white bream (Blicca bjoerkna (L.)) and rudd (Scardinius erythrophthalmus (L.))) and their potential predators (pike (Esox lucius (L.)) and ...

  7. Browse Title Index

    African Journals Online (AJOL)

    Items 301 - 350 of 708 ... Vol 30, No 1 (2005), Histological studies to evaluate gonad development in Barbus neefi (Cyprinidae), the sidespot barb, from South Africa, Abstract. W Vlok. Vol 41, No 2 (2016), Histopathological biomarkers and genotoxicity in gill and liver tissues of Nile tilapia Oreochromis niloticus from a polluted ...

  8. Contrasting phylogeography of two Western Palaearctic fish parasites despite similar life cycles

    Czech Academy of Sciences Publication Activity Database

    Perrot-Minnot, M. J.; Špakulová, M.; Wattier, R.; Kotlík, Petr; Düsen, S.; Aydoğdu, A.; Tougard, C.

    2018-01-01

    Roč. 45, č. 1 (2018), s. 101-115 ISSN 0305-0270 R&D Projects: GA MŠk EF15_003/0000460 Institutional support: RVO:67985904 Keywords : amphipod * British Islands * comparative phylogeography * Cyprinidae * Danube Subject RIV: EG - Zoology Impact factor: 4.248, year: 2016

  9. Fish fauna of the Camp dels Ninots locality (Pliocene; Caldes de Malavella, province of Girona, Spain) - first results with notes on palaeoecology and taphonomy

    Czech Academy of Sciences Publication Activity Database

    Přikryl, Tomáš; Gómez de Soler, B.; Oms, O.; Roubach, S.; Blain, H-A.; Agustí, J.

    2016-01-01

    Roč. 28, č. 3 (2016), s. 347-357 ISSN 0891-2963 Institutional support: RVO:67985831 Keywords : Camp dels Ninots * Cyprinidae * maar lake * NE Spain * Pliocene * Teleostei Subject RIV: DB - Geology ; Mineralogy Impact factor: 1.556, year: 2016

  10. Phylogenetic relationships of Acheilognathidae (Cypriniformes: Cyprinoidea) as revealed from evidence of both nuclear and mitochondrial gene sequence variation: Evidence for necessary taxonomic revision in the family and the identification of cryptic species

    Czech Academy of Sciences Publication Activity Database

    Chang, H.-C.; Li, F.; Shao, K.-T.; Lin, Y.-S.; Morosawa, T.; Kim, S.; Koo, H.; Kim, W.; Lee, J.-S.; He, S.; Smith, Carl; Reichard, Martin; Miya, M.; Sado, T.; Uehara, K.; Lavoué, S.; Chen, W.-J.; Mayden, R. L.

    2014-01-01

    Roč. 81, December (2014), s. 182-194 ISSN 1055-7903 Institutional support: RVO:68081766 Keywords : Acheilognathinae * Cyprinidae * Cryptic species * Nuclear loci * Cytochrome b * European bitterling Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.916, year: 2014

  11. African Journal of Aquatic Science - Vol 30, No 1 (2005)

    African Journals Online (AJOL)

    Growth, reproduction and population structure of Diplotaxodon limnothrissa in the southeast arm of Lake Malawi · EMAIL FULL TEXT EMAIL FULL TEXT ... Histological studies to evaluate gonad development in Barbus neefi (Cyprinidae), the sidespot barb, from South Africa · EMAIL FULL TEXT EMAIL FULL TEXT

  12. Karyoevolutionary and karyosystematic considerations on ...

    African Journals Online (AJOL)

    Cytogenetic studies have helped in clarifying the problem of disagreement amongst taxonomists on the identity of a given species. Cytogenetic studies were performed on two fishes of the genus Schizothorax viz. Schizothorax curvifrons Heckel and Schizothorax niger Heckel (Cyprinidae: Schizothoracinae) obtained from ...

  13. Fulltext PDF

    Indian Academy of Sciences (India)

    HONG GAO LIU

    Saurogobio dabryi Bleeker, 1871 (Chinese lizard gudgeon) is a diploid fish (2n = 50), belongs to the family Cyprinidae, subfamily Gobioninae (Tang et al. 2011) and distributed in the east Asia (Dai and Yang 2002). As an indispensable component of the food web, it is essential for the stabil- ity and integrity of the Yangtze ...

  14. Identification And Study Of Fish Species In Karkheh River (Iran

    Directory of Open Access Journals (Sweden)

    Khoshnood Zahra

    2014-10-01

    Full Text Available For the investigation of fish from Karkheh River, sampling was performed in a six month period from August 2014 to January 2015. All sampled fish were measured for biometrical values (length and weight. General results of the sampling and identification of the fish showed the presence of 14 species from four fish families of Cyprinidae, Mugilidae, Siluridae and Macrostomidae, out of which the Cyprinidae family were the most frequent of the sampled fish. The most significant abundance belongs to Cyprinus carpio. The fish sampled in the present study were: Liza abu, Ctenopharyngodon idella, Barbel sp., Cyprinion macrostomum, Barbus sharpeyi, Hypophthalmichthys molitrix, Barbus esocinus, Barbus barbulus, Barbus luteus, Barbus grypus, Cyprinus carpio, Silurus triostegus, Mastacembelus circumcinctus and Capoeta trutta. Shannon Index results showed that the fish biodiversity in the studyed area followed a uniform path and additionally that the considered area at the studied period has good fish biodiversity.

  15. in ovarian fluid

    African Journals Online (AJOL)

    Jane

    2011-09-28

    Sep 28, 2011 ... salmon and trout oocytes can be successfully stored for more times, even for 2 days in Caspian brown trout. (Niksirat et al., 2007a) and for 9 days at 2 to 3°C in rainbow trout (Niksirat et al., 2007b). Kutum (Rutilus frisii kutum) is a cyprinidae endemic to the Caspian Sea. It is a semi-migratory anadromous fish.

  16. Résultats ichthyologiques des voyages scientifiques de Monsieur le Professeur Dr. A. W. Nieuwenhuis dans le centre de Bornéo (1898 et 1900)

    NARCIS (Netherlands)

    Popta, C.M.L.

    1905-01-01

    Dans quelques genres de Cyprinidae, c.-à-d. le Dangila, l’ Osteochilus et le Crossochilus famille des Cyprininae, qui appartiennent à la sousle nombre des écailles de la ligne latérale est plus petit dans les espèces spéciales que dans les espèces anciennes. Le Tylognathus a augmenté ce nombre de 2

  17. The effect of Ligula intestinalis on blood sex steroid hormones, gonadal tissue and some other biological parameters changes of Chalcalburnus mossulensis in Vahdat dam of Kordestan-Iran

    OpenAIRE

    Khanghah, Ali Parsa

    2010-01-01

    Chalcalburnus mossulensis from the cyprinidae family is one of the indigenous fish in Gheshlag Lake of Kordestan-Iran. Ligula intestinalis is one of the infective parasites of this fish. In this study, the effect of this parasite on some biological aspects of this fish like weight, length, PI, CF, GSR, blood sex steroid hormones and gonadal tissue, was investigated. During one year, by seasonal sampling, 144 fish sample from mentioned species were collected using trap net. By considering the ...

  18. The effect of depth, distance from dam and habitat on spatial distribution of fish in an artificial reservoir

    Czech Academy of Sciences Publication Activity Database

    Prchalová, Marie; Kubečka, Jan; Čech, Martin; Frouzová, Jaroslava; Draštík, Vladislav; Hohausová, Eva; Jůza, Tomáš; Kratochvíl, Michal; Matěna, Josef; Peterka, Jiří; Říha, Milan; Tušer, Michal; Vašek, Mojmír

    2009-01-01

    Roč. 18, č. 2 (2009), s. 247-260 ISSN 0906-6691 R&D Projects: GA AV ČR(CZ) 1QS600170504; GA ČR(CZ) GA206/07/1392; GA MZe(CZ) QH81046 Institutional research plan: CEZ:AV0Z60170517 Keywords : horizontal distribution * vertical distribution * gillnet * reservoir * Cyprinidae * Percidae * redundancy analysis Subject RIV: EH - Ecology, Behaviour Impact factor: 1.512, year: 2009

  19. Expression of Major histocompatibility complex genes in carp (Cyprinus carpio L.)

    NARCIS (Netherlands)

    Rodrigues, P.N.S.

    1996-01-01


    The common carp ( Cyprinus carpio L.) has been the experimental animal of choice because many features of the immune system of this Cyprinid fish have been well characterized. The immune system consists of an integrated set of organs containing

  20. Low genetic differentiation among morphologically distinct ...

    African Journals Online (AJOL)

    Labeobarbus altianalis and L. bynni bynni are hexaploid cyprinid fishes in the genus Labeobarbus. In the Great Lakes region of Africa, these two large-bodied barbs exhibit considerable morphological variations. Their intraspecific classification, currently based on geographical distribution and morphological variation, is of ...

  1. Seasonal migration determined by a trade-off between predator avoidance and growth

    DEFF Research Database (Denmark)

    Brönmark, C.; Skov, Christian; Brodersen, J.

    2008-01-01

    on commercially important fish species, such as salmon and trout. However, seasonal mass-migrations may occur also among other freshwater fish, e.g. in cyprinids that leave lakes and migrate into streams and wetlands in the fall and return back to the lake in spring. In a conceptual model, we hypothesized...

  2. Preliminary examination of hatching season and growth of ...

    African Journals Online (AJOL)

    The hatching period and the growth of Malawian cyprinid Engraulicypris sardella larvae and juveniles collected from two sites along the southwestern shoreline of Lake Malawi were investigated using otolith increment information. The lapillus was used to determine the age in days following previous studies. The hatching ...

  3. Winter activity of common bream (Abramis brama L.) in a European reservoir

    Czech Academy of Sciences Publication Activity Database

    Jurajda, Pavel; Roche, Kevin Francis; Halačka, Karel; Mrkvová, Markéta; Zukal, Jan

    (2018) ISSN 0969-997X R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:68081766 Keywords : cyprinid behaviour * fish metabolism * predation risk * reservoir fisheries * telemetry * thermocline Impact factor: 1.327, year: 2016

  4. Trypanoplasma borreli cysteine proteinase activities support a conservation of function with respect to digestion of host proteins in common carp

    NARCIS (Netherlands)

    Ruszczyk, Aleksandra; Forlenza, Maria; Joerink, Maaike; Ribeiro, Carla M. S.; Jurecka, Patrycja; Wiegertjes, Geert F.

    2008-01-01

    Trypanoplasma borreli is an extracellular parasite that is transmitted by a leech vector and is naturally found in the blood of cyprinid fish. High parasitemia and associated severe anemia together with splenomegaly are typical of infection of common carp, Cyprinus carpio L. Papain-like cysteine

  5. Distribution, size and feeding habits of Nanaethiops unitaeniatus ...

    African Journals Online (AJOL)

    Aspects of the ecology of Nanaethiops unitaeniatus were studied in a rainforest wetland ecosystem (Mfangmfang pond) in Uyo, Nigeria. This fish occurred through out the year and was commonly found in association with the cyprinid, Barbus callipterus. They were often found in shoals of 20 or more fishes. They remained in ...

  6. Validation of the periodicity of growth increment deposition in ...

    African Journals Online (AJOL)

    Validation of the periodicity of growth increment deposition in otoliths from the larval and early juvenile stages of two cyprinids from the Orange–Vaal river ... Linear regression models were fitted to the known age post-fertilisation and the age estimated using increment counts to test the correspondence between the two for ...

  7. Suppression of invasive topmouth gudgeon Pseudorasbora parva by native pike Esox lucius in ponds

    NARCIS (Netherlands)

    Lemmens, P.; Mergeay, J.; Vanhove, T.; De Meester, L.; Declerck, S.A.J.

    2015-01-01

    1. Asian topmouth gudgeon Pseudorasbora parva has been recognized as a highly invasive cyprinid fish species in Europe that can present risk to native fish communities. 2. The present study aimed to investigate whether a native piscivorous fish, pike Esox lucius, is able to reduce the establishment

  8. Heavy metals in two host-parasite systems: tapeworm vs. fish

    Czech Academy of Sciences Publication Activity Database

    Baruš, Vlastimil; Šimková, A.; Prokeš, Miroslav; Peňáz, Milan; Vetešník, Lukáš

    2012-01-01

    Roč. 81, č. 3 (2012), s. 313-317 ISSN 0001-7213 Institutional research plan: CEZ:AV0Z60930519 Institutional support: RVO:68081766 Keywords : Ligula * Bathybothrium * cyprinid fishes Subject RIV: EH - Ecology, Behaviour Impact factor: 0.393, year: 2012

  9. Koi herpesvirus disease (khvd) surveillance and diagnosis

    DEFF Research Database (Denmark)

    Way, K.; Bergmann, S. M.; Engelsma, Marc

    together skills and experience on this fish disease from different parts of Europe. In the report of the meeting sent to the commission important issues concerning serology and cyprinid herpesvirus variants were raised. We hope that our recommendations to resolve these issues will be considered...

  10. Rapid detection and identification of viral and bacterial fish pathogens using a DNA array‐based multiplex assay

    DEFF Research Database (Denmark)

    Lievens, B.; Frans, I.; Heusdens, C.

    2011-01-01

    for the simultaneous detection and identification of all cyprinid herpesviruses (CyHV‐1, CyHV‐2 and CyHV‐3) and some of the most important fish pathogenic Flavobacterium species, including F. branchiophilum, F. columnare and F. psychrophilum. For virus identification, the DNA polymerase and helicase genes were...

  11. Aquatic Habitat Studies on the Lower Mississippi River, River Mile 480 to 530. Report 6. Larval Fish Studies-Pilot Report.

    Science.gov (United States)

    1981-04-01

    larval fish were collected: unidentified clupeids, unidentified cyprinids, Carpiodes spp., Menidia audens , Lepomis spp., unidentified centrarchids, and...bars, was rare in both abandoned channels and oxbow lakes. 69. Menidia audens and Morone spp. were common in the abandoned channel habitat and rare in

  12. Molecular detection of northern leatherside chub (Lepidomeda copei) DNA in environmental samples

    Science.gov (United States)

    Joseph C. Dysthe; Kellie J. Carim; Thomas W. Franklin; Dave Kikkert; Michael K. Young; Kevin S. McKelvey; Michael K. Schwartz

    2018-01-01

    The northern leatherside chub (Lepidomeda copei) is a cyprinid fish native to the Snake River, Green River, and Bonneville basins of the western United States. Population declines prompted the development of a multistate conservation agreement and strategy, which emphasized the need to reliably delineate its current distribution and monitor its status. To facilitate...

  13. Trypanoplasma borreli cystein proteinase activities support a conservation of function with respect to digestion of host proteins in common carp

    NARCIS (Netherlands)

    Ruszczyk, A.; Forlenza, M.; Joerink, M.; Ribeiro, C.M.S.; Jurecka, P.M.; Wiegertjes, G.F.

    2008-01-01

    Trypanoplasma borreli is an extracellular parasite that is transmitted by a leech vector and is naturally found in the blood of cyprinid fish. High parasitemia and associated severe anemia together with splenomegaly are typical of infection of common carp, Cyprinus carpio L. Papain-like cysteine

  14. Isolation of innate immune response genes, expression analysis, polymorphism identification and development of genetic marker for linkage analysis in common carp (Cyprinus carpio)

    Science.gov (United States)

    Common carp are economically important foodfish worldwide. Over the past few years, carp aquaculture has suffered from enormous losses to a disease caused by cyprinid herpesvirus 3 (CyHV-3). A recent study reported that common carp strains/crossbreds have differential resistance to CyHV-3, suggest...

  15. SNP discovery and development of genetic markers for mapping immune response genes in common carp (Cyprinus carpio)

    Science.gov (United States)

    Single nucleotide polymorphisms (SNPs) in immune response genes have been reported as markers for susceptibility to infectious diseases in human and livestock. A disease caused by cyprinid herpesvirus 3 (CyHV-3) is highly contagious and virulent in common carp (Cyprinus carpio). With the aim to de...

  16. SNP discovery and marker development for disease resistance candidate genes in common carp (Cyprinus carpio)

    Science.gov (United States)

    Single nucleotide polymorphisms (SNPs) in immune response genes have been reported as markers of susceptibility to infectious diseases in human and livestock. A disease caused by cyprinid herpes virus 3 (CyHV-3) is highly contagious and virulent in common carp. With the aim to investigate the gene...

  17. Littoral age 0+ fish distribution in relation to multi-scale spatial heterogeneity of a deep-valley reservoir

    Czech Academy of Sciences Publication Activity Database

    Kratochvíl, Michal; Mrkvička, Tomáš; Vašek, Mojmír; Peterka, Jiří; Čech, Martin; Draštík, Vladislav; Jůza, Tomáš; Matěna, Josef; Muška, Milan; Seďa, Jaromír; Znachor, Petr; Kubečka, Jan

    2012-01-01

    Roč. 696, č. 1 (2012), s. 185-198 ISSN 0018-8158 R&D Projects: GA MZe(CZ) QH81046 Institutional research plan: CEZ:AV0Z60170517 Keywords : habitat preference * electrofishing * growing season * slope steepness * percids * cyprinids Subject RIV: EH - Ecology, Behaviour Impact factor: 1.985, year: 2012

  18. Importation of CyHV-2-infected goldfish into the Netherlands

    NARCIS (Netherlands)

    Ito, Takafumi; Kurita, Jun; Haenen, Olga L.M.

    2017-01-01

    Cyprinid herpesvirus 2 (CyHV-2) is known as the causative agent of herpesviral haematopoietic necrosis in goldfish Carassius auratus auratus. However, the virus has also been detected in Prussian carp C. gibelio and crucian carp C. carassius from European and Asian countries. To prevent spread of

  19. Species integrity enhanced by a predation cost to hybrids in the wild

    DEFF Research Database (Denmark)

    Nilsson, P. A.; Hulthén, Kaj; Chapman, Ben B.

    2017-01-01

    Species integrity can be challenged, and even eroded, if closely related species can hybridize and produce fertile offspring of comparable fitness to that of parental species. The maintenance of newly diverged or closely related species therefore hinges on the establishment and effectiveness of pre...... barrier to gene flow in the wild. Cyprinid fishes commonly produce fertile, viable hybrid offspring and therefore make excellent study organisms to investigate ecological costs to hybrids. We electronically tagged two freshwater cyprinid fish species (roach Rutilus rutilus and bream Abramis brama...... to directly test for a predation cost to hybrids in the wild. Hybrid individuals were found significantly more susceptible to cormorant predation than individuals from either parental species. Such ecological selection against hybrids contributes to species integrity, and can enhance species diversification....

  20. Fish-T1K (Transcriptomes of 1,000 Fishes) Project: large-scale transcriptome data for fish evolution studies.

    Science.gov (United States)

    Sun, Ying; Huang, Yu; Li, Xiaofeng; Baldwin, Carole C; Zhou, Zhuocheng; Yan, Zhixiang; Crandall, Keith A; Zhang, Yong; Zhao, Xiaomeng; Wang, Min; Wong, Alex; Fang, Chao; Zhang, Xinhui; Huang, Hai; Lopez, Jose V; Kilfoyle, Kirk; Zhang, Yong; Ortí, Guillermo; Venkatesh, Byrappa; Shi, Qiong

    2016-01-01

    Ray-finned fishes (Actinopterygii) represent more than 50 % of extant vertebrates and are of great evolutionary, ecologic and economic significance, but they are relatively underrepresented in 'omics studies. Increased availability of transcriptome data for these species will allow researchers to better understand changes in gene expression, and to carry out functional analyses. An international project known as the "Transcriptomes of 1,000 Fishes" (Fish-T1K) project has been established to generate RNA-seq transcriptome sequences for 1,000 diverse species of ray-finned fishes. The first phase of this project has produced transcriptomes from more than 180 ray-finned fishes, representing 142 species and covering 51 orders and 109 families. Here we provide an overview of the goals of this project and the work done so far.

  1. Long-term development of fish populations in the Římov Reservoir

    Czech Academy of Sciences Publication Activity Database

    Říha, Milan; Kubečka, Jan; Vašek, Mojmír; Seďa, Jaromír; Mrkvička, Tomáš; Prchalová, Marie; Matěna, Josef; Hladík, M.; Čech, Martin; Draštík, Vladislav; Frouzová, Jaroslava; Hohausová, E.; Jarolím, Oldřich; Jůza, Tomáš; Kratochvíl, Michal; Peterka, Jiří; Tušer, Michal

    2009-01-01

    Roč. 16, č. 2 (2009), s. 121-129 ISSN 0969-997X R&D Projects: GA AV ČR(CZ) 1QS600170504 Grant - others:EU(XE) GOCE-CT-2003-505298 Institutional research plan: CEZ:AV0Z60170517 Keywords : succession * reservoir * biodiversity * cyprinids * perch * stability Subject RIV: EH - Ecology, Behaviour Impact factor: 1.264, year: 2009

  2. Explosive diversification following a benthic to pelagic shift in freshwater fishes

    OpenAIRE

    Hollingsworth, Phillip R; Simons, Andrew M; Fordyce, James A; Hulsey, C Darrin

    2013-01-01

    Background Interspecific divergence along a benthic to pelagic habitat axis is ubiquitous in freshwater fishes inhabiting lentic environments. In this study, we examined the influence of this habitat axis on the macroevolution of a diverse, lotic radiation using mtDNA and nDNA phylogenies for eastern North America’s most species-rich freshwater fish clade, the open posterior myodome (OPM) cyprinids. We used ancestral state reconstruction to identify the earliest benthic to pelagic transition ...

  3. Suppression of invasive topmouth gudgeon Pseudorasbora parva by native pike Esox lucius in ponds

    OpenAIRE

    Lemmens, Pieter; Mergeay, Joachim; Vanhove, Tom; De Meester, Luc; Declerck, Steven A. J

    2015-01-01

    1. Asian topmouth gudgeon Pseudorasbora parva has been recognized as a highly invasive cyprinid fish species in Europe that can present risk to native fish communities. 2. The present study aimed to investigate whether a native piscivorous fish, pike Esox lucius, is able to reduce the establishment success and invasiveness of topmouth gudgeon Pseudorasbora parva in shallow ponds. We performed a large scale, replicated whole-pond experiment in which ponds were spontaneously colonized by to...

  4. Importance of small fishes and invasive crayfish in otter Lutra lutra diet in an English chalk stream

    OpenAIRE

    Britton, J.R.; Berry, M.; Sewell, S.; Lees, C.; Reading, P.

    2017-01-01

    The diet composition of the European otter Lutra lutra was assessed using spraint analysis in the Hampshire Avon, a lowland chalk stream in Southern England, over an 18-month period. Small cyprinid fishes were the main prey item taken in all seasons, with bullhead Cottus gobio and stone loach Barbatula barbatula also important; there were relatively few larger fishes of interest to fisheries found. There were significant seasonal differences in diet composition by season, with signal crayfish...

  5. Impact of flood on distribution of bathypelagic perch fry layer along the longitudinal profile of large canyon-shaped reservoir

    Czech Academy of Sciences Publication Activity Database

    Čech, Martin; Kubečka, Jan; Frouzová, Jaroslava; Draštík, Vladislav; Kratochvíl, Michal; Jarošík, Jiří

    2007-01-01

    Roč. 70, č. 4 (2007), s. 1109-1119 ISSN 0022-1112. [Annual Meeting of the American Fisheries Society /136/. Lake Placid, 10.09.2006-14.09.2006] R&D Projects: GA ČR(CZ) GA206/06/1371 Institutional research plan: CEZ:AV0Z60170517 Keywords : Orlík Reservoir * shoaling * echosounder * cyprinids * Perca fluviatilis * Sander lucioperca Subject RIV: EG - Zoology Impact factor: 1.404, year: 2007

  6. [A catalog of fish specimens preserved within Kunming Institute of Zoology, Chinese Academy of Sciences].

    Science.gov (United States)

    Du, Li-Na; Chen, Xiao-Yong; Yang, Jun-Xing

    2013-08-01

    As of 2013, some 178 fish type species and 2131 type specimens belonging to 4 orders and 11 families were currently being preserved at the Kunming Natural History Museum of Zoology, located as art of the Kunming Institute of Zoology, Chinese Academy of Sciences. These specimens were collected from across western China, includingYunnan, Sicuan, Guizhou, Guangxi, Hunan, Chongqi, Gansu and Xinjiang. In general, most species are Cyprinidae (71 species and 1103 specimens), followed by Nemacheilidae (52 species and 556 specimens). For the convenience of research and communication, the present paper presents a detailed list of fish type species preserved in the Kunming Natural History Museum of Zoology.

  7. A PRELIMINARY HISTOLOGICAL STUDY ON OVARIUM DEVELOPMENT IN MIRROR CARP AND SCALED CARP (Cyprinus carpio L., 1758) INTRODUCED INTO GELINGÜLLÜ RESERVOIR, TURKEY

    OpenAIRE

    Erkmen, Belda; Kırankaya, Şerife Gülsün

    2016-01-01

    Carp(Cyprinus carpio) is the most common species of the Cyprinidae familyand is among the important species commercially caught in the GelingüllüReservoir, Yozgat, Turkey. In this study, the reproductive biology of mirrorcarp and scaled carp in Gelingüllü Reservoir was studied in terms of thegonadosomatic index (GSI), fecundity, and ovarian histology. Ovaries wereclassified into 5 maturity stages based on histological data, as follows: 1.Chromatin-nucleolus stage (oocyte diameter is 20-60 µm)...

  8. In vivo and in vitro activities of the seed extract of Piper guineense Schum. and Thonn. against skin and gill monogenean parasites of gold fish (Carassius auratus auratus).

    Science.gov (United States)

    Ekanem, A P; Wang, M; Simon, J E; Obiekezie, A I; Morah, F

    2004-10-01

    Methanol extracts of the seeds of Piper guineense (Piperaceae) were active against gold fish (Carassius auratus auratus L. Pisces Cyprinidae) monogenean parasites. The seed extract of P. guineense was administered at different concentrations (0.5-2.0 mg/L) under in vivo and in vitro conditions. There was a higher efficacy of the effects of the extracts against fish parasites under in vitro situations than under in vivo. Three major compounds (piperanine, N-isobutyl (E,E)-2,4 decadienamide and Deltaalpha,beta-dihydrowasanine) were identified from the seed extract of Piper guineense by LC-MS analysis. Copyright 2004 John Wiley & Sons, Ltd.

  9. Trophic interaction between topmouth gudgeon (Pseudorasbora parva and the co-occurring species during summer in the Dniprodzerzhynsk reservoir

    Directory of Open Access Journals (Sweden)

    Didenko A.V.

    2015-01-01

    Full Text Available Topmouth gudgeon, Pseudorasbora parva is the most common invasive fish in Europe, which can compete with the juveniles of valuable commercial fishes. The goal of this work is to study the diet of topmouth gudgeon and trophic relationships with some native fishes inhabiting the littoral zone of the Dniprodzerzhynsk reservoir. The obtained relatively low values of diet overlaps between topmouth gudgeon and other co-occurring cyprinids such as juvenile roach, Rutilus rutilus; silver bream, Blicca bjoerkna; rudd, Scardinius erythrophthalmus; Prussian carp, Carassius gibelio and bleak, Abramis brama, indicate that it is not a serious food competitor for them, especially commercially valuable ones. On the contrary, high diet overlaps were observed among some native cyprinids such as juvenile roach, silver bream, rudd, and bleak. The major prey items of topmouth gudgeon were Chydorus sphaericus and chironomids, whereas other cyprinids selected Bosmina spp. This peculiarity may be due to different vertical distributions of these fish in the littoral zone of the reservoir, where topmouth gudgeon inhabit near-bottom water layers, where they preyed on near-bottom and benthic zooplankters and chironomids, while juvenile roach, silver bream, rudd, and juvenile and adult bleak live in higher water layers, where they preyed on pelagic zooplankton.

  10. Land, lake, and fish: Investigation of fish remains from Gesher Benot Ya'aqov (paleo-Lake Hula).

    Science.gov (United States)

    Zohar, Irit; Biton, Rebecca

    2011-04-01

    The question of whether or not pre-modern hominins were responsible for the accumulation of fish remains is discussed through analyses of remains recovered from two lacustrine facies (I-4 and I-5) from Area A of the Acheulian site of Gesher Benot Ya'aqov (GBY) in the Jordan Rift Valley, Israel. The fish remains provide the first glimpse into the naturally accumulated fish assemblage from the fluctuating shores of a lake that had been continually exploited by early hominins some 780,000 years ago. Preliminary analysis of the remains show that thirteen of the seventeen species native to Lake Hula were identified at GBY. These represent three of the five freshwater fish families native to the lake: Cyprinidae (carps), Cichlidae (tilapini, St. Peter's fish), and Clariidae (catfish). From a taphonomical perspective, a significant difference is found between the two lithofacies (Layers I-4 and I-5) in terms of species composition, richness, diversity, and skeleton completeness. It appears that the fish remains recovered from Layer I-4 (clay) are better preserved than those from Layer I-5 (coquina). In both lithofacies, Cyprinidae are highly abundant while Cichlidae and Clariidae are rare and under-represented, especially when compared to the Lake Hula fishery report from the 1950s. All of these identified species may have contributed significantly to the diet of GBY hominins. Copyright © 2010 Elsevier Ltd. All rights reserved.

  11. The Pet Trade as a Source of Invasive Fish in Taiwan

    Directory of Open Access Journals (Sweden)

    Shih-Hisung Liang

    2006-06-01

    Full Text Available In this study, ornamental fish available for purchase at pet stores in Taiwan were compared to those documented as having invaded the island’s ecosystems. In total, 293 pet fish species belonging to 48 families were recorded. The top five families that included the most pet fish species were the Cichilidae (79 species, Callichthyidae (38, Characidae (37, Cyprinidae (20, and Loricariidae (16. Among the 48 families, five families, documented as having invasion records, are the Cichilidae, Cyprinidae, Loricariidae, Belontiidae, and Poeciliidae. Except for the Cichilidae, the proportion of invasive species to total species of other four families were at least equal to or greater than 10%, with the Belontiidae displaying the highest proportion at 66.6%. The Belontiidae also demonstrated a significantly higher rate of documented invasive species than did the other families. Families with a greater number of recorded pet fish species also presented a significant positive correlation with their documented invasive species if the 20 families with only a single surveyed species were excluded. Forcing retailers to foster home program, urging aquariums to display how to eliminate fish escaping from captivity, and instituting a higher custom duty or higher purchasing tax were proposed to eliminate future introductions of pet fish species.

  12. Emergence of the Zoonotic Biliary Trematode Pseudamphistomum truncatum in Grey Seals (Halichoerus grypus) in the Baltic Sea

    Science.gov (United States)

    Bergman, Anders; Bignert, Anders; Höglund, Johan; Lundström, Karl; Strömberg, Annika; Bäcklin, Britt-Marie

    2016-01-01

    The biliary trematode Pseudamphistomum truncatum parasitizes a wide range of fish-eating mammals, including humans. Here we report the emergence of this parasite in grey seals (Halichoerus grypus) in the Baltic Sea. One hundred eighty-three of 1 554 grey seals (11.9%) examined from 2002–2013 had detectable hepatobiliary trematode infection. Parasite identification was confirmed as P. truncatum by sequencing the ITS2 region of a pool of five to 10 trematodes from each of ten seals collected off the coast of seven different Swedish counties. The proportion of seals parasitized by P. truncatum increased significantly over time and with increasing age of seals. Males were 3.1 times more likely to be parasitized than females and animals killed in fishery interactions were less likely to be parasitized than animals found dead or hunted. There was no significant difference in parasitism of seals examined from the Gulf of Bothnia versus those examined from the Baltic Proper. Although the majority of infections were mild, P. truncatum can cause severe hepatobiliary disease and resulted in liver failure in at least one seal. Because cyprinid fish are the second intermediate host for opisthorchiid trematodes, diets of grey seals from the Baltic Sea were analysed regarding presence of cyprinids. The proportion of gastrointestinal tracts containing cyprinid remains was ten times higher in seals examined from 2008 to 2013 (12.2%) than those examined from 2002 to 2007 (1.2%) and coincided with a general increase of trematode parasitism in the host population. The emergence and relatively common occurrence of P. truncatum in grey seals signals the presence of this parasite in the Baltic Sea ecosystem and demonstrates how aquatic mammals can serve as excellent sentinels of marine ecosystem change. Investigation of drivers behind P. truncatum emergence and infection risk for other mammals, including humans, is highly warranted. PMID:27755567

  13. Emergence of the Zoonotic Biliary Trematode Pseudamphistomum truncatum in Grey Seals (Halichoerus grypus in the Baltic Sea.

    Directory of Open Access Journals (Sweden)

    Aleksija S Neimanis

    Full Text Available The biliary trematode Pseudamphistomum truncatum parasitizes a wide range of fish-eating mammals, including humans. Here we report the emergence of this parasite in grey seals (Halichoerus grypus in the Baltic Sea. One hundred eighty-three of 1 554 grey seals (11.9% examined from 2002-2013 had detectable hepatobiliary trematode infection. Parasite identification was confirmed as P. truncatum by sequencing the ITS2 region of a pool of five to 10 trematodes from each of ten seals collected off the coast of seven different Swedish counties. The proportion of seals parasitized by P. truncatum increased significantly over time and with increasing age of seals. Males were 3.1 times more likely to be parasitized than females and animals killed in fishery interactions were less likely to be parasitized than animals found dead or hunted. There was no significant difference in parasitism of seals examined from the Gulf of Bothnia versus those examined from the Baltic Proper. Although the majority of infections were mild, P. truncatum can cause severe hepatobiliary disease and resulted in liver failure in at least one seal. Because cyprinid fish are the second intermediate host for opisthorchiid trematodes, diets of grey seals from the Baltic Sea were analysed regarding presence of cyprinids. The proportion of gastrointestinal tracts containing cyprinid remains was ten times higher in seals examined from 2008 to 2013 (12.2% than those examined from 2002 to 2007 (1.2% and coincided with a general increase of trematode parasitism in the host population. The emergence and relatively common occurrence of P. truncatum in grey seals signals the presence of this parasite in the Baltic Sea ecosystem and demonstrates how aquatic mammals can serve as excellent sentinels of marine ecosystem change. Investigation of drivers behind P. truncatum emergence and infection risk for other mammals, including humans, is highly warranted.

  14. Comparative Genomics of Carp Herpesviruses

    Science.gov (United States)

    Kurobe, Tomofumi; Gatherer, Derek; Cunningham, Charles; Korf, Ian; Fukuda, Hideo; Hedrick, Ronald P.; Waltzek, Thomas B.

    2013-01-01

    Three alloherpesviruses are known to cause disease in cyprinid fish: cyprinid herpesviruses 1 and 3 (CyHV1 and CyHV3) in common carp and koi and cyprinid herpesvirus 2 (CyHV2) in goldfish. We have determined the genome sequences of CyHV1 and CyHV2 and compared them with the published CyHV3 sequence. The CyHV1 and CyHV2 genomes are 291,144 and 290,304 bp, respectively, in size, and thus the CyHV3 genome, at 295,146 bp, remains the largest recorded among the herpesviruses. Each of the three genomes consists of a unique region flanked at each terminus by a sizeable direct repeat. The CyHV1, CyHV2, and CyHV3 genomes are predicted to contain 137, 150, and 155 unique, functional protein-coding genes, respectively, of which six, four, and eight, respectively, are duplicated in the terminal repeat. The three viruses share 120 orthologous genes in a largely colinear arrangement, of which up to 55 are also conserved in the other member of the genus Cyprinivirus, anguillid herpesvirus 1. Twelve genes are conserved convincingly in all sequenced alloherpesviruses, and two others are conserved marginally. The reference CyHV3 strain has been reported to contain five fragmented genes that are presumably nonfunctional. The CyHV2 strain has two fragmented genes, and the CyHV1 strain has none. CyHV1, CyHV2, and CyHV3 have five, six, and five families of paralogous genes, respectively. One family unique to CyHV1 is related to cellular JUNB, which encodes a transcription factor involved in oncogenesis. To our knowledge, this is the first time that JUNB-related sequences have been reported in a herpesvirus. PMID:23269803

  15. Quantitative PCR Assays for Detecting Loach Minnow (Rhinichthys cobitis and Spikedace (Meda fulgida in the Southwestern United States.

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    Joseph C Dysthe

    Full Text Available Loach minnow (Rhinichthys cobitis and spikedace (Meda fulgida are legally protected with the status of Endangered under the U.S. Endangered Species Act and are endemic to the Gila River basin of Arizona and New Mexico. Efficient and sensitive methods for monitoring these species' distributions are critical for prioritizing conservation efforts. We developed quantitative PCR assays for detecting loach minnow and spikedace DNA in environmental samples. Each assay reliably detected low concentrations of target DNA without detection of non-target species, including other cyprinid fishes with which they co-occur.

  16. To migrate, or not to migrate: partial diel horizontal migration of fish in a temperate freshwater reservoir

    Czech Academy of Sciences Publication Activity Database

    Muška, Milan; Tušer, Michal; Frouzová, Jaroslava; Draštík, Vladislav; Čech, Martin; Jůza, Tomáš; Kratochvíl, Michal; Mrkvička, T.; Peterka, Jiří; Prchalová, Marie; Říha, Milan; Vašek, Mojmír; Kubečka, Jan

    2013-01-01

    Roč. 707, č. 1 (2013), s. 17-28 ISSN 0018-8158. [Česká ichtyologická konference /13./. Červená nad Vltavou, 24.10.2012-26.10.2012] R&D Projects: GA MZe(CZ) QH81046; GA MŠk(CZ) EE2.3.20.0204 Institutional support: RVO:60077344 Keywords : diel activity * diurnal migration * individual decision making * horizontal hydroacoustics * purse seining * cyprinids Subject RIV: EH - Ecology, Behaviour Impact factor: 2.212, year: 2013

  17. Molecular characterization of Sphaerospora molnari (Myxozoa), the agent of gill sphaerosporosis in common carp (Cyprinus carpio carpio)

    Czech Academy of Sciences Publication Activity Database

    Eszterbauer, E.; Sipos, D.; Forró, B.; Bartošová, Pavla; Holzer, Astrid S.

    2013-01-01

    Roč. 104, č. 1 (2013), s. 59-67 ISSN 0177-5103 R&D Projects: GA ČR GBP505/12/G112; GA ČR(CZ) GPP506/11/P724 Grant - others:GA AV ČR(CZ) M200961205 Institutional support: RVO:60077344 Keywords : Sphaerosporosis * Histozoic myxozoans * Blood stages * Cyprinids * 18S rDNA * Phylogeny * In situ hybridization Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.586, year: 2013

  18. 'Who's who' in renal sphaerosporids (Bivalvulida: Myxozoa) from common carp, Prussian carp and goldfish - molecular identification of cryptic species, blood stages and new members of Sphaerospora sensu stricto

    Czech Academy of Sciences Publication Activity Database

    Holzer, Astrid S.; Bartošová, Pavla; Pecková, Hana; Tyml, Tomáš; Atkinson, S.; Bartholomew, J.; Sipos, D.; Eszterbauer, E.; Dyková, Iva

    2013-01-01

    Roč. 140, JAN 2013 (2013), s. 46-60 ISSN 0031-1820 R&D Projects: GA ČR(CZ) GPP506/11/P724; GA ČR GBP505/12/G112 Grant - others:Hungarian Scientifc Research Fund(HU) OTKA K75873 Institutional research plan: CEZ:AV0Z60220518 Institutional support: RVO:60077344 Keywords : Sphaerospora * Myxozoa * cyprinid * morphometry * cryptic speciation * ribosomal DNA * molecular identification * blood stages * multi-species infection Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.350, year: 2013

  19. SOME COMPARATIVE BIOMETRIC ASPECTS IN ARISTICHTHYS NOBILIS AND HYPOPHTHALMICHTHYS MOLITRIX

    Directory of Open Access Journals (Sweden)

    GABRIELA VASILE

    2008-10-01

    Full Text Available The paper performs a comparative analysis of some biometric (total length, standard length, head length and caudal footstalks length in two cultured cyprinids species grown in a controlled system, namely: Aristichthys nobilis (bighead carp and Hypophthalmichthys molitrix (silver carp, of various ages (starting with the first up to the fourth growth summer. The results obtained evidenced that, along the four growing stages, the representatives of Hypophthalmichthys molitrix show slightly higher values than those of Aristichthys genus for the total and standard bodily length, while for the head length the situation is reversed.

  20. The cranial cartilages of teleosts and their classification.

    OpenAIRE

    Benjamin, M

    1990-01-01

    The structure and distribution of cartilages has been studied in 45 species from 24 families. The resulting data have been used as a basis for establishing a new classification. A cartilage is regarded as 'cell-rich' if its cells or their lacunae occupy more than half of the tissue volume. Five classes of cell-rich cartilage are recognised (a) hyaline-cell cartilage (common in the lips of bottom-dwelling cyprinids) and its subtypes fibro/hyaline-cell cartilage, elastic/hyaline-cell cartilage ...

  1. Fish genomes provide novel insights into the evolution of vertebrate secretin receptors and their ligand.

    Science.gov (United States)

    Cardoso, João C R; Félix, Rute C; Trindade, Marlene; Power, Deborah M

    2014-12-01

    The secretin receptor (SCTR) is a member of Class 2 subfamily B1 GPCRs and part of the PAC1/VPAC receptor subfamily. This receptor has long been known in mammals but has only recently been identified in other vertebrates including teleosts, from which it was previously considered to be absent. The ligand for SCTR in mammals is secretin (SCT), an important gastrointestinal peptide, which in teleosts has not yet been isolated, or the gene identified. This study revises the evolutionary model previously proposed for the secretin-GPCRs in metazoan by analysing in detail the fishes, the most successful of the extant vertebrates. All the Actinopterygii genomes analysed and the Chondrichthyes and Sarcopterygii fish possess a SCTR gene that shares conserved sequence, structure and synteny with the tetrapod homologue. Phylogenetic clustering and gene environment comparisons revealed that fish and tetrapod SCTR shared a common origin and diverged early from the PAC1/VPAC subfamily group. In teleosts SCTR duplicated as a result of the fish specific whole genome duplication but in all the teleost genomes analysed, with the exception of tilapia (Oreochromis niloticus), one of the duplicates was lost. The function of SCTR in teleosts is unknown but quantitative PCR revealed that in both sea bass (Dicentrarchus labrax) and tilapia (Oreochromis mossambicus) transcript abundance is high in the gastrointestinal tract suggesting it may intervene in similar processes to those in mammals. In contrast, no gene encoding the ligand SCT was identified in the ray-finned fishes (Actinopterygii) although it was present in the coelacanth (lobe finned fish, Sarcopterygii) and in the elephant shark (holocephalian). The genes in linkage with SCT in tetrapods and coelacanth were also identified in ray-finned fishes supporting the idea that it was lost from their genome. At present SCTR remains an orphan receptor in ray-finned fishes and it will be of interest in the future to establish why SCT was

  2. Posthodiplostomatosis in a fishpond in Serbia

    Directory of Open Access Journals (Sweden)

    Marković Maja

    2012-01-01

    Full Text Available Posthodiplostomatosis (black spot disease is a disease of young freshwater fish species of families Cyprinidae and Cobitidae, resulting from infection with metacercaria, which is the penultimate developmental stage of Posthodiplostomum cuticola. Metacercaria give rise to black cysts located primarily in the skin, muscles and on the fins. The disease caused by P. cuticola in certain fish species is first detected in a fishpond in Serbia, as presented in the current work, whilst in natural waters it has been existing for many years. Diagnosis of the infection in the grass carp (Ctenopharyngodon idella and the bighead carp (Aristichthys nobilis was based on the occurrence of dark discolorations and cysts of the parasite on the fins. Cysts measured approximately 1 mm in diameter, were confirmed by histopathological means. Posthodiplostomatosis occurred in fishponds in which preventive measures were not implemented, i.e. where the nurseries were not dried up and mechanically cleaned.

  3. Keragaman ikan di Danau Cala, Kabupaten Musi Banyuasin Sumatera Selatan

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    Jifi Abu Ammar

    2014-12-01

    Full Text Available The aim of his studi was to evaluate the fish fauna in Danau Cala in dry season, transition season, and the tide season. The survey was conducted 3 times on July 2013, October 2013 and January 2014 as representative of dry, transition and tide seasons. The fish sampling was conducted by gillnets and fish traps. A total of 35 fish species were recorded during the study; 23 species in dry season, it was dominated by tembakang fish (Helostoma temminckii from family Helosmatidae, Sepat siam fish(Trichogaster pectoralis from Belontiidae family, and snakehead fish (Channa striata of Channidae family. A total of 16 species were recorded on transitional seasons where baung fish (Hemibagrus nemurus of the family Bagridae, and seluang fish (Rasbora sp of the family Cyprinidae were predominant. In addition, 15 fish species were recorded on tide season which was dominated by baung fish (Hemibagrus nemurus and tendon fish (Mystus sabanus of family Bagridae.

  4. The Key Role of Epigenetics in the Persistence of Asexual Lineages

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    Emilie Castonguay

    2012-01-01

    Full Text Available Asexual organisms, often perceived as evolutionary dead ends, can be long-lived and geographically widespread. We propose that epigenetic mechanisms could play a crucial role in the evolutionary persistence of these lineages. Genetically identical organisms could rely on phenotypic plasticity to face environmental variation. Epigenetic modifications could be the molecular mechanism enabling such phenotypic plasticity; they can be influenced by the environment and act at shorter timescales than mutation. Recent work on the asexual vertebrate Chrosomus eos-neogaeus (Pisces: Cyprinidae provides broad insights into the contribution of epigenetics in genetically identical individuals. We discuss the extension of these results to other asexual organisms, in particular those resulting from interspecific hybridizations. We finally develop on the evolutionary relevance of epigenetic variation in the context of heritability.

  5. Barcoding of fresh water fishes from Pakistan.

    Science.gov (United States)

    Karim, Asma; Iqbal, Asad; Akhtar, Rehan; Rizwan, Muhammad; Amar, Ali; Qamar, Usman; Jahan, Shah

    2016-07-01

    DNA bar-coding is a taxonomic method that uses small genetic markers in organisms' mitochondrial DNA (mt DNA) for identification of particular species. It uses sequence diversity in a 658-base pair fragment near the 5' end of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene as a tool for species identification. DNA barcoding is more accurate and reliable method as compared with the morphological identification. It is equally useful in juveniles as well as adult stages of fishes. The present study was conducted to identify three farm fish species of Pakistan (Cyprinus carpio, Cirrhinus mrigala, and Ctenopharyngodon idella) genetically. All of them belonged to family cyprinidae. CO1 gene was amplified. PCR products were sequenced and analyzed by bioinformatic software. Conspecific, congenric, and confamilial k2P nucleotide divergence was estimated. From these findings, it was concluded that the gene sequence, CO1, may serve as milestone for the identification of related species at molecular level.

  6. Polymorphic microsatellite markers for the endangered fish, the slender shiner Pseudopungtungia tenuicorpa and cross-species amplification across five related species.

    Science.gov (United States)

    Kim, K S; Moon, S J; Han, S H; Kim, K Y; Bang, I C

    2016-09-02

    The slender shiner Pseudopungtungia tenuicorpa (Cypriniformes; Cyprinidae; Gobioninae) is an endangered freshwater fish species endemic to Korea. The current strategies for its conservation involve the study of population genetic characters and identification of management units. These strategies require suitable molecular markers to study genetic diversity and genetic structure. Here, we developed nine polymorphic microsatellite markers for P. tenuicorpa for the first time by applying an enrichment method from a size-selected genomic library. The developed microsatellite markers produced a total of 101 alleles (average 11.2). The observed and expected heterozygosities averaged 0.805 and 0.835, respectively. Among the nine identified markers, five markers showed successful amplification across five related Korean Gobioninae species. Thus, the microsatellite markers developed in this study will be useful to establish conservation strategies for both P. tenuicorpa and other related species.

  7. Sperm motility in fishes. (II) Effects of ions and osmolality: a review.

    Science.gov (United States)

    Alavi, Sayyed Mohammad Hadi; Cosson, Jacky

    2006-01-01

    The spermatozoa of most fish species are immotile in the testis and seminal plasma. Therefore, motility is induced after the spermatozoa are released into the aqueous environment during natural reproduction or into the diluent during artificial reproduction. There are clear relationships between seminal plasma composition and osmolality and the duration of fish sperm motility. Various parameters such as ion concentrations (K+, Na+, and Ca2+), osmotic pressure, pH, temperature and dilution rate affect motility. In the present paper, we review the roles of these ions on sperm motility in Salmonidae, Cyprinidae, Acipenseridae and marine fishes, and their relationship with seminal plasma composition. Results in the literature show that: 1. K+ is a key ion controlling sperm motility in Salmonidae and Acipenseridae in combination with osmotic pressure; this control is more simple in other fish species: sperm motility is prevented when the osmotic pressure is high (Cyprinidae) or low (marine fishes) compared to that of the seminal fluid. 2. Cations (mostly divalent, such as Ca2+) are antagonistic with the inhibitory effect of K+ on sperm motility. 3. In many species, Ca2+ influx and K+ or Na+ efflux through specific ionic channels change the membrane potential and eventually lead to an increase in cAMP concentration in the cell, which constitutes the initiation signal for sperm motility in Salmonidae. 4. Media that are hyper- and hypo-osmotic relative to seminal fluid trigger sperm motility in marine and freshwater fishes, respectively. 5. The motility of fish spermatozoa is controlled through their sensitivity to osmolality and ion concentrations. This phenomenon is related to ionic channel activities in the membrane and governs the motility mechanisms of axonemes.

  8. A consensus linkage map of the grass carp (Ctenopharyngodon idella based on microsatellites and SNPs

    Directory of Open Access Journals (Sweden)

    Li Jiale

    2010-02-01

    Full Text Available Abstract Background Grass carp (Ctenopharyngodon idella belongs to the family Cyprinidae which includes more than 2000 fish species. It is one of the most important freshwater food fish species in world aquaculture. A linkage map is an essential framework for mapping traits of interest and is often the first step towards understanding genome evolution. The aim of this study is to construct a first generation genetic map of grass carp using microsatellites and SNPs to generate a new resource for mapping QTL for economically important traits and to conduct a comparative mapping analysis to shed new insights into the evolution of fish genomes. Results We constructed a first generation linkage map of grass carp with a mapping panel containing two F1 families including 192 progenies. Sixteen SNPs in genes and 263 microsatellite markers were mapped to twenty-four linkage groups (LGs. The number of LGs was corresponding to the haploid chromosome number of grass carp. The sex-specific map was 1149.4 and 888.8 cM long in females and males respectively whereas the sex-averaged map spanned 1176.1 cM. The average resolution of the map was 4.2 cM/locus. BLAST searches of sequences of mapped markers of grass carp against the whole genome sequence of zebrafish revealed substantial macrosynteny relationship and extensive colinearity of markers between grass carp and zebrafish. Conclusions The linkage map of grass carp presented here is the first linkage map of a food fish species based on co-dominant markers in the family Cyprinidae. This map provides a valuable resource for mapping phenotypic variations and serves as a reference to approach comparative genomics and understand the evolution of fish genomes and could be complementary to grass carp genome sequencing project.

  9. Functional feeding traits as predictors of invasive success of alien freshwater fish species using a food-fish model.

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    Leopold A J Nagelkerke

    Full Text Available Invasions of Ponto-Caspian fish species into north-western European river basins accelerated since the opening of the Rhine-Main-Danube Canal in 1992. Since 2002, at least five Ponto-Caspian alien fish species have arrived in The Netherlands. Four species belong to the Gobiidae family (Neogobius fluviatilis, Neogobius melanostomus, Ponticola kessleri, and Proterorhinus semilunaris and one to the Cyprinidae family (Romanogobio belingi. These species are expected to be potentially deleterious for the populations of four native benthic fish species: Gobio gobio (Cyprinidae, Barbatula barbatula (Nemacheilidae, Cottus perifretum, and C. rhenanus (Cottidae. Invasion success may be dependent on competitive trophic interactions with native species, which are enabled and/or constrained by feeding-related morphological traits. Twenty-two functional feeding traits were measured in nine species (in total 90 specimens. These traits were quantitatively linked to the mechanical, chemical and behavioral properties of a range of aquatic resource categories, using a previously developed food-fish model (FFM. The FFM was used to predict the trophic profile (TP of each fish: the combined capacities to feed on each of the resource types. The most extreme TPs belonged to three alien species, indicating that they were most specialized among the studied species. Of these three, only P. kessleri overlapped with the two native Cottus species, indicating potential trophic competition. N. fluviatilis and R. belingi did not show any overlap, indicating that there is low trophic competition. The two remaining alien goby species (N. melanostomus and P. semilunaris had average TPs and could be considered generalist feeders. They overlapped with each other and with G. gobio and B. barbatula, indicating potential trophic competition. This study suggests that both generalist and specialist species can be successful invaders. Since the FFM predicts potential interactions between

  10. Iktiofauna Sungai Sangkir Kabupaten Rokan Hulu Provinsi Riau (Ichthyofauna of Sangkir River, Rokan Hulu District Riau Province

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    Nunuk Dian Pranata

    2016-12-01

    Full Text Available This study was conducted in Sangkir river branch of Rokan Kiri river Sangkir village, Rokan Hulu district, Riau Province from May of July 2016. The aim of this study was to inventory the fish species in Sangkir river branch of Rokan Kiri River with direct observation (survey. Five sampling locations were determined purposely based on the environmental condition that could be represented by the river condition. A total of 288 individuals of fish belonging to 3 orders, 7 families, 13 genera and 16 species were recorded in this study, namely Barbodes balleroides, Barbodes gonionotus, Barbichthys leavis, Channa striata, Cyclocheilichthys apogon, Labiobarbus fasciatus, Mystus nigriceps, Ompok eugeneiatus, Osteochilus hasseltii, Osteochilus mycrocephalus, Pangio semicincta, Pristolepis grooti, Thynnichthys polylepis, Trichogaster leerii, Trichogaster trichopterus and Trichopsis vittata. Cyprinidae was the most abundance fish in this study. Penelitian ini telah dilaksanakan di sungai Sangkir, anak sungai Rokan Kiri, desa Sangkir, Kabupaten Rokan Hulu, Provinsi Riau pada bulan Mei sampai Juli 2016. Penelitian ini bertujuan untuk mengetahui jenis-jenis ikan di sungai Sangkir anak Sungai Rokan Kiri dengan metode pengamatan langsung (survei dengan penetapan stasiun pengambilan sampel secara purposive sampling. Penetapan stasiun berdasarkan kondisi lingkungan yang terdiri dari 5 stasiun penelitian. Hasil penelitian  didapatkan sebanyak 288 individu ikan yang terdiri dari 3 ordo, 7 famili 13 genus dan 16 spesies. Spesies yang didapatkan yaitu Barbodes balleroides, Barbodes gonionotus, Barbichthys leavis, Channa striata, Cyclocheilichthys apogon, Labiobarbus fasciatus, Mystus nigriceps, Ompok eugeneiatus, Osteochilus hasseltii, Osteochilus mycrocephalus, Pangio semicincta, Pristolepis grooti, Thynnichthys polylepis Trichogaster leerii, Trichogaster trichopterus dan Trichopsis vittata. Cyprinidae merupakan kelompok ikan yang paling banyak dalam penelitian ini.

  11. Functional feeding traits as predictors of invasive success of alien freshwater fish species using a food-fish model.

    Science.gov (United States)

    Nagelkerke, Leopold A J; van Onselen, Eline; van Kessel, Nils; Leuven, Rob S E W

    2018-01-01

    Invasions of Ponto-Caspian fish species into north-western European river basins accelerated since the opening of the Rhine-Main-Danube Canal in 1992. Since 2002, at least five Ponto-Caspian alien fish species have arrived in The Netherlands. Four species belong to the Gobiidae family (Neogobius fluviatilis, Neogobius melanostomus, Ponticola kessleri, and Proterorhinus semilunaris) and one to the Cyprinidae family (Romanogobio belingi). These species are expected to be potentially deleterious for the populations of four native benthic fish species: Gobio gobio (Cyprinidae), Barbatula barbatula (Nemacheilidae), Cottus perifretum, and C. rhenanus (Cottidae). Invasion success may be dependent on competitive trophic interactions with native species, which are enabled and/or constrained by feeding-related morphological traits. Twenty-two functional feeding traits were measured in nine species (in total 90 specimens). These traits were quantitatively linked to the mechanical, chemical and behavioral properties of a range of aquatic resource categories, using a previously developed food-fish model (FFM). The FFM was used to predict the trophic profile (TP) of each fish: the combined capacities to feed on each of the resource types. The most extreme TPs belonged to three alien species, indicating that they were most specialized among the studied species. Of these three, only P. kessleri overlapped with the two native Cottus species, indicating potential trophic competition. N. fluviatilis and R. belingi did not show any overlap, indicating that there is low trophic competition. The two remaining alien goby species (N. melanostomus and P. semilunaris) had average TPs and could be considered generalist feeders. They overlapped with each other and with G. gobio and B. barbatula, indicating potential trophic competition. This study suggests that both generalist and specialist species can be successful invaders. Since the FFM predicts potential interactions between species, it

  12. Explosive diversification following a benthic to pelagic shift in freshwater fishes.

    Science.gov (United States)

    Hollingsworth, Phillip R; Simons, Andrew M; Fordyce, James A; Hulsey, C Darrin

    2013-12-17

    Interspecific divergence along a benthic to pelagic habitat axis is ubiquitous in freshwater fishes inhabiting lentic environments. In this study, we examined the influence of this habitat axis on the macroevolution of a diverse, lotic radiation using mtDNA and nDNA phylogenies for eastern North America's most species-rich freshwater fish clade, the open posterior myodome (OPM) cyprinids. We used ancestral state reconstruction to identify the earliest benthic to pelagic transition in this group and generated fossil-calibrated estimates of when this shift occurred. This transition could have represented evolution into a novel adaptive zone, and therefore, we tested for a period of accelerated lineage accumulation after this historical habitat shift. Ancestral state reconstructions inferred a similar and concordant region of our mtDNA and nDNA based gene trees as representing the shift from benthic to pelagic habitats in the OPM clade. Two independent tests conducted on each gene tree suggested an increased diversification rate after this inferred habitat transition. Furthermore, lineage through time analyses indicated rapid early cladogenesis in the clade arising after the benthic to pelagic shift. A burst of diversification followed the earliest benthic to pelagic transition during the radiation of OPM cyprinids in eastern North America. As such, the benthic/pelagic habitat axis has likely influenced the generation of biodiversity across disparate freshwater ecosystems.

  13. Probiotics in carp fish nutrition

    Directory of Open Access Journals (Sweden)

    Ljubojević Dragana

    2013-01-01

    Full Text Available Uncontrolled use of antibiotics in aquaculture caused the appearance and spreading of pathogens resistance to antibiotics, what consequently drove up to the necesity of finding new technologies for protecting aquatic organisms from pathogens. Probiotics are aimed to reduce antibiotics application, and furthermore they have an important role not only in disease prevention but also in efficiency of food utilization and improvement of production parameters. Carp fish species are economically most significant in Republic of Serbia, therefore this work summarizes the results of previous studies of antibiotics application in this kind in particular. There is also pointed out to numerous harmful side effects of antibiotics use in aquaculture, and described the previous results of investigations on mechanism of probiotics effects and specificity of their use in this field as well. Beside this, there are summarized the results that show positive influence of probiotics in cyprinides nutrition on production performance, haematological parameters, course of experimental infection, activity of digestive enzymes. Special attention is paid to criteria for proper selection of probiotics in cyprinides production. [Projekat Ministarstva nauke Republike Srbije, br. TP 31011: Uticaj kvaliteta komponenata u ishrani ciprinida na kvalitet mesa, gubitke i ekonomičnost proizvodnje

  14. The draft genome of blunt snout bream (Megalobrama amblycephala) reveals the development of intermuscular bone and adaptation to herbivorous diet

    Science.gov (United States)

    Liu, Han; Chen, Chunhai; Gao, Zexia; Min, Jiumeng; Gu, Yongming; Jian, Jianbo; Jiang, Xiewu; Cai, Huimin; Ebersberger, Ingo; Xu, Meng; Zhang, Xinhui; Chen, Jianwei; Luo, Wei; Chen, Boxiang; Chen, Junhui; Liu, Hong; Li, Jiang; Lai, Ruifang; Bai, Mingzhou; Wei, Jin; Yi, Shaokui; Wang, Huanling; Cao, Xiaojuan; Zhou, Xiaoyun; Zhao, Yuhua; Wei, Kaijian; Yang, Ruibin; Liu, Bingnan; Zhao, Shancen; Fang, Xiaodong

    2017-01-01

    Abstract The blunt snout bream Megalobrama amblycephala is the economically most important cyprinid fish species. As an herbivore, it can be grown by eco-friendly and resource-conserving aquaculture. However, the large number of intermuscular bones in the trunk musculature is adverse to fish meat processing and consumption. As a first towards optimizing this aquatic livestock, we present a 1.116-Gb draft genome of M. amblycephala, with 779.54 Mb anchored on 24 linkage groups. Integrating spatiotemporal transcriptome analyses, we show that intermuscular bone is formed in the more basal teleosts by intramembranous ossification and may be involved in muscle contractibility and coordinating cellular events. Comparative analysis revealed that olfactory receptor genes, especially of the beta type, underwent an extensive expansion in herbivorous cyprinids, whereas the gene for the umami receptor T1R1 was specifically lost in M. amblycephala. The composition of gut microflora, which contributes to the herbivorous adaptation of M. amblycephala, was found to be similar to that of other herbivores. As a valuable resource for the improvement of M. amblycephala livestock, the draft genome sequence offers new insights into the development of intermuscular bone and herbivorous adaptation. PMID:28535200

  15. A comparison of complete mitochondrial genomes of silver carp hypophthalmichthys molitrix and bighead carp hypophthalmichthys nobilis: Implications for their taxonomic relationship and phylogeny

    Science.gov (United States)

    Li, S.-F.; Xu, J.-W.; Yang, Q.-L.; Wang, C.H.; Chen, Q.; Chapman, D.C.; Lu, G.

    2009-01-01

    Based upon morphological characters, Silver carp Hypophthalmichthys molitrix and bighead carp Hypophthalmichthys nobilis (or Aristichthys nobilis) have been classified into either the same genus or two distinct genera. Consequently, the taxonomic relationship of the two species at the generic level remains equivocal. This issue is addressed by sequencing complete mitochondrial genomes of H. molitrix and H. nobilis, comparing their mitogenome organization, structure and sequence similarity, and conducting a comprehensive phylogenetic analysis of cyprinid species. As with other cyprinid fishes, the mitogenomes of the two species were structurally conserved, containing 37 genes including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA (tRNAs) genes and a putative control region (D-loop). Sequence similarity between the two mitogenomes varied in different genes or regions, being highest in the tRNA genes (98??8%), lowest in the control region (89??4%) and intermediate in the protein-coding genes (94??2%). Analyses of the sequence comparison and phylogeny using concatenated protein sequences support the view that the two species belong to the genus Hypophthalmichthys. Further studies using nuclear markers and involving more closely related species, and the systematic combination of traditional biology and molecular biology are needed in order to confirm this conclusion. ?? 2009 The Fisheries Society of the British Isles.

  16. Threatened species richness along a Himalayan elevational gradient: quantifying the influences of human population density, range size, and geometric constraints.

    Science.gov (United States)

    Paudel, Prakash Kumar; Sipos, Jan; Brodie, Jedediah F

    2018-02-07

    A crucial step in conserving biodiversity is to identify the distributions of threatened species and the factors associated with species threat status. In the biodiversity hotspot of the Himalaya, very little is known about which locations harbour the highest diversity of threatened species and whether diversity of such species is related to area, mid-domain effects (MDE), range size, or human density. In this study, we assessed the drivers of variation in richness of threatened birds, mammals, reptiles, actinopterygii, and amphibians along an elevational gradient in Nepal Himalaya. Although geometric constraints (MDE), species range size, and human population density were significantly related to threatened species richness, the interaction between range size and human population density was of greater importance. Threatened species richness was positively associated with human population density and negatively associated with range size. In areas with high richness of threatened species, species ranges tend to be small. The preponderance of species at risk of extinction at low elevations in the subtropical biodiversity hotspot could be due to the double impact of smaller range sizes and higher human density.

  17. DNA barcoding in Mexico: an introduction.

    Science.gov (United States)

    Elías-Gutiérrez, M; León-Regagnon, V

    2013-11-01

    DNA barcoding has become an important current scientific trend to the understanding of the world biodiversity. In the case of mega-diverse hot spots like Mexico, this technique represents an important tool for taxonomists, allowing them to concentrate in highlighted species by the barcodes instead of analyzing entire sets of specimens. This tendency resulted in the creation of a national network named Mexican Barcode of Life (MEXBOL) which main goals are to train students, and to promote the interaction and collective work among researchers interested in this topic. As a result, the number of records in the Barcode of Life Database (BOLD) for some groups, such as the Mammalia, Actinopterygii, Polychaeta, Branchiopoda, Ostracoda, Maxillopoda, Nematoda, Pinophyta, Ascomycota and Basidiomycota place Mexico among the top ten countries in the generation of these data. This special number presents only few of the many interesting findings in this region of the world, after the use of this technique and its integration with other methodologies. © 2013 John Wiley & Sons Ltd.

  18. Feeding dynamics, consumption rates and daily ration of wahoo Acanthocybium solandri in Indo-Pacific waters.

    Science.gov (United States)

    Perelman, J N; Schmidt, K N; Haro, I; Tibbetts, I R; Zischke, M T

    2017-05-01

    This study reports the diet composition of 363 wahoo Acanthocybium solandri captured from the Indo-Pacific. The study also provides the first estimates of consumption and daily ration for the species worldwide, which are important parameters for ecosystem models and may improve ecosystem-based fisheries management. Thirty-four prey taxa were identified from A. solandri stomachs with Scombridae having the highest relative importance. Actinopterygii comprised 96% of the total prey wet mass, of which 29% were epipelagic fishes, with 22% alone from Scombridae. There was no significant relationship between fish size and the size of prey items consumed. Feeding intensity, as measured by stomach fullness, did not significantly differ either among seasons or reproductive activity. The mean daily consumption rate was estimated as 344 g day -1 , which corresponded to a mean daily ration of 2·44% body mass day -1 . The results from this study suggest A. solandri is an opportunistic predator similar to other pelagic piscivores, worldwide. © 2017 The Fisheries Society of the British Isles.

  19. Immunocytochemical identification of adenohypophyseal cells in the pirarucu (Arapaima gigas), an Amazonian basal teleost.

    Science.gov (United States)

    Borella, M I; Venturieri, R; Mancera, J M

    2009-03-01

    The adenohypophysis (AH) of juvenile pirarucu (Arapaima gigas), a representative species of the Osteoglossomorpha (bonytongue fishes, one of the oldest living groups of the teleosts), was studied using histochemical and immunocytochemical methods. The AH is comprised of the pars distalis (PD), without a clear distinction between rostral pars distalis (RPD) and proximal pars distalis (PPD), and the pars intermedia (PI). The neurohypophysis (NH) is positioned on top of the PD and penetrates and branches into the PI. In the most rostral dorsal portion of the PD, adrenocorticotropic cells and fusiform gonadotropic cells were found. In the central PD, scarce prolactin-producing cells and growth-hormone-producing cells were located mainly in the dorsal part, whereas round gonadotropic cells were abundant in the ventral portion of this region. Human thyrotropin immunoreactive cells were not found in the entire AH. In the PI, melanotropic, some adrenocorticotropic, and somatolactin-producing cells were located intermingled surrounding the neurohypophyseal branches. Our results showed that the A. gigas pituitary has some basal characteristics between the ancient Actinopterygii and the more derived teleosts.

  20. Comparative phylogenetic analysis of male alternative reproductive tactics in ray-finned fishes.

    Science.gov (United States)

    Mank, Judith E; Avise, John C

    2006-06-01

    Using comparative phylogenetic analysis, we analyzed the evolution of male alternative reproductive tactics (MARTs) in ray-finned fishes (Actinopterygii). Numerous independent origins for each type of MART (involving sneaker males, female mimics, pirates, and satellite males) indicate that these behaviors have been highly labile across actinopterygiian evolution, consistent with a previous notion that convergent selection in fishes can readily mold the underlying suites of reproductive hormones into similar behaviors. The evolutionary appearance of MARTs was significantly correlated with the presence of sexually selected traits in bourgeois males (P = 0.001) but not with the presence of male parental care. This suggests that MARTs often arise from selection on some males to circumvent bourgeois male investment in mate monopolization, rather than to avoid male brood care per se. We found parsimony evidence for an evolutionary progression of MARTs wherein sneaking is usually the evolutionary precursor to the presumably more complex MARTs of female mimicry and cooperative satellite behavior. Nest piracy appears not to be part of this evolutionary progression, possibly because its late onset in the life cycle of most ray-finned fishes reduces the effects of selection on this reproductive tactic.

  1. The extraordinary biology and development of marsupial frogs (Hemiphractidae) in comparison with fish, mammals, birds, amphibians and other animals.

    Science.gov (United States)

    Del Pino, Eugenia M

    2018-01-03

    The study of oogenesis and early development of frogs belonging to the family Hemiphractidae provide important comparison to the aquatic development of other frogs, such as Xenopus laevis, because reproduction on land characterizes the Hemiphractidae. In this review, the multinucleated oogenesis of the marsupial frog Flectonotus pygmaeus (Hemiphractidae) is analyzed and interpreted. In addition, the adaptations associated with the incubation of embryos in the pouch of the female marsupial frog Gastrotheca riobambae (Hemiphractidae) and the embryonic development of this frog are summarized. Moreover, G. riobambae gastrulation is compared with the gastrulation modes of Engystomops randi and Engystomops coloradorum (Leptodactylidae); Ceratophrys stolzmanni (Ceratophryidae); Hyalinobatrachium fleischmanni and Espadarana callistomma (Centrolenidae); Ameerega bilinguis, Dendrobates auratus, Epipedobates anthonyi, Epipedobates machalilla, Epipedobates tricolor, and Hyloxalus vertebralis (Dendrobatidae); Eleutherodactylus coqui (Terrarana: Eleutherodactylidae), and X. laevis (Pipidae). The comparison indicated two modes of frog gastrulation. In X. laevis and in frogs with aquatic reproduction, convergent extension begins during gastrulation. In contrast, convergent extension occurs in the post-gastrula of frogs with terrestrial reproduction. These two modes of gastrulation resemble the transitions toward meroblastic cleavage found in ray-finned fishes (Actinopterygii). In spite of this difference, the genes that guide early development seem to be highly conserved in frogs. I conclude that the shift of convergent extension to the post-gastrula accompanied the diversification of frog egg size and terrestrial reproductive modes. Copyright © 2018 Elsevier B.V. All rights reserved.

  2. Seasonal and Spatial Environmental Influence on Opisthorchis viverrini Intermediate Hosts, Abundance, and Distribution: Insights on Transmission Dynamics and Sustainable Control.

    Directory of Open Access Journals (Sweden)

    Christina Sunyoung Kim

    2016-11-01

    Full Text Available Opisthorchis viverrini (Ov is a complex-life-cycle trematode affecting 10 million people in SEA (Southeast Asia. Human infection occurs when infected cyprinid fish are consumed raw or undercooked. Ov requires three hosts and presents two free-living parasitic stages. As a consequence Ov transmission and infection in intermediate and human hosts are strongly mediated by environmental factors and understanding how environmental variability influences intermediate host abundance is critical. The objectives of this study were 1 to document water parameters, intermediate hosts abundance and infection spatio-temporal variation, 2 to assess their causal relationships and identify windows of transmission risk.Fish and snails were collected monthly for one year at 12 sites in Lawa Lake, an Ov-endemic region of Khon Kaen Province in Northeast Thailand. Physicochemical water parameters [pH, temperature (Tp, dissolved oxygen (DO, Salinity, electrical conductivity (EC, total dissolved solid (TDS, nitrite nitrogen (NO2-N, lead (Pb, total coliform bacteria (TCB and fecal coliform bacteria (FCB] were measured. Multivariate analyses, linear models and kriging were used to characterize water parameter variation and its influence on host abundance and infection prevalence. We found that sampling sites could be grouped in three clusters and discriminated along a nitrogen-salinity gradient where higher levels in the lake's southern region predicted higher Bithynia relative abundance (P<0.05 and lower snail and fish species diversity (P<0.05. Highest Bithynia abundance occurred during rainy season (P<0.001, independently of site influence. Cyprinids were the most abundant fish family and higher cyprinid relative abundance was found in areas with higher Bithynia relative abundance (P<0.05. Ov infection in snails was anecdotal while Ov infection in fish was higher in the southern region (P<0.001 at sites showing high FCB.Our results indicate that water contamination

  3. Broad-scale sampling of primary freshwater fish populations reveals the role of intrinsic traits, inter-basin connectivity, drainage area and latitude on shaping contemporary patterns of genetic diversity

    Directory of Open Access Journals (Sweden)

    Carla Sousa-Santos

    2016-02-01

    Full Text Available Background. Worldwide predictions suggest that up to 75% of the freshwater fish species occurring in rivers with reduced discharge could be extinct by 2070 due to the combined effect of climate change and water abstraction. The Mediterranean region is considered to be a hotspot of freshwater fish diversity but also one of the regions where the effects of climate change will be more severe. Iberian cyprinids are currently highly endangered, with over 68% of the species raising some level of conservation concern. Methods. During the FISHATLAS project, the Portuguese hydrographical network was extensively covered (all the 34 river basins and 47 sub-basins in order to contribute with valuable data on the genetic diversity distribution patterns of native cyprinid species. A total of 188 populations belonging to 16 cyprinid species of Squalius, Luciobarbus, Achondrostoma, Iberochondrostoma, Anaecypris and Pseudochondrostoma were characterized, for a total of 3,678 cytochrome b gene sequences. Results. When the genetic diversity of these populations was mapped, it highlighted differences among populations from the same species and between species with identical distribution areas. Factors shaping the contemporary patterns of genetic diversity were explored and the results revealed the role of latitude, inter-basin connectivity, migratory behaviour, species maximum size, species range and other species intrinsic traits in determining the genetic diversity of sampled populations. Contrastingly, drainage area and hydrological regime (permanent vs. temporary seem to have no significant effect on genetic diversity. Species intrinsic traits, maximum size attained, inter-basin connectivity and latitude explained over 30% of the haplotype diversity variance and, generally, the levels of diversity were significantly higher for smaller sized species, from connected and southerly river basins. Discussion. Targeting multiple co-distributed species of primary

  4. Spring viraemia of carp (SVC) in the UK: the road to freedom.

    Science.gov (United States)

    Taylor, N G H; Peeler, E J; Denham, K L; Crane, C N; Thrush, M A; Dixon, P F; Stone, D M; Way, K; Oidtmann, B C

    2013-08-01

    Spring viraemia of carp (SVC) is a disease of international importance that predominantly affects cyprinid fish and can cause significant mortality. In the United Kingdom (UK), SVC was first detected in 1977 with further cases occurring in fisheries, farms, wholesale and retail establishments throughout England and Wales (but not Scotland, where few cyprinid populations exist, nor Northern Ireland where SVC has never been detected) over the subsequent 30 years. Following a control and eradication programme for the disease initiated in 2005, the UK was recognised free of the disease in 2010. This study compiles historic records of SVC cases in England and Wales with a view to understanding its routes of introduction and spread, and assessing the effectiveness of the control and eradication programme in order to improve contingency plans to prevent and control future disease incursions in the cyprinid fish sectors. Between 1977 and 2010 the presence of SVC was confirmed on 108 occasions, with 65 of the cases occurring in sport fisheries and the majority of the remainder occurring in the ornamental fish sector. The study found that throughout the history of SVC in the UK, though cases were widely distributed, their occurrence was sporadic and the virus did not become endemic. All evidence indicates that SVC was not able to persist under UK environmental conditions, suggesting that the majority of cases were a result of new introductions to the UK as opposed to within-country spread. The control and eradication programme adopted in 2005 was highly effective and two years after its implementation cases of SVC ceased. Given the non-persistent nature of the pathogen the most important aspect of the control programme focused on preventing re-introduction of the virus to the UK. Despite the effectiveness of these controls against SVC, this approach is likely to be less effective against more persistent pathogens such as koi herpesvirus, which are likely to require more

  5. The first record of the invasive Asian fish tapeworm (Schyzocotyle acheilognathi from an endemic cichlid fish in Madagascar

    Directory of Open Access Journals (Sweden)

    Scholz T.

    2018-03-01

    Full Text Available The Asian fish tapeworm, Schyzocotyle acheilognathi (Yamaguti, 1934 (Cestoda: Bothriocephalidea, is an invasive parasite of freshwater fishes that have been reported from more than 200 freshwater fish worldwide. It was originally described from a small cyprinid, Acheilognathus rombeus, in Japan but then has spread, usually with carp, minnows or guppies, to all continents including isolated islands such as Hawaii, Puerto Rico, Cuba or Sri Lanka. In the present account, we report the first case of the infection of a native cichlid fish, Ptychochromis cf. inornatus (Perciformes: Cichlidae, endemic to Madagascar, with S. acheilognathi. The way of introduction of this parasite to the island, which is one of the world’s biodiversity hotspots, is briefly discussed.

  6. First report of the intracellular fish parasite Sphaerothecum destruens associated with the invasive topmouth gudgeon (Pseudorasbora parva in France

    Directory of Open Access Journals (Sweden)

    Charrier Amélie

    2016-01-01

    Full Text Available Sphaerothecum destruens has emerged as a serious parasite of fish. Its life cycle, as well as its association with Asian cyprinids, allows it to infect a wide range of hosts. The topmouth gudgeon (Pseudorasbora parva, an invasive species that has rapidly colonized Europe, has been shown to be a healthy carrier of the parasite. However, in France, the presence of S. destruens and its possible association with P. parva have not yet been demonstrated. Here, we screened topmouth gudgeon DNA for S. destruens using PCR amplification of an 18S rRNA gene fragment of the parasite. Sequencing and phylogenetic analysis confirmed the presence of S. destruens in the invasive fish species. Our results suggest that P. parva can be a potent vector of the parasite, and has the potential to become a major ecological and economic threat to the French fish population.

  7. Influence of light intensity and spectral composition of artificial light at night on melatonin rhythm and mRNA expression of gonadotropins in roach Rutilus rutilus.

    Science.gov (United States)

    Brüning, Anika; Hölker, Franz; Franke, Steffen; Kleiner, Wibke; Kloas, Werner

    2018-02-01

    In this study we investigated the influence of artificial light at night (ALAN) of different intensities (0, 1, 10, 100 lx) and different colours (blue, green, red) on the daily melatonin rhythm and mRNA expression of gonadotropins in roach Rutilus rutilus, a ubiquitous cyprinid, which occur in standing and moderately flowing freshwater habitats of central Europe. Melatonin concentrations were significantly lowered under nocturnal white light already at 1 lx. Low intensity blue, green and red ALAN lowered the melatonin levels significantly in comparison to a dark control. We conclude that ALAN can disturb melatonin rhythms in roach at very low intensities and at different wavelengths and thus light pollution in urban waters has the potential to impact biological rhythms in fish. However, mRNA expression of gonadotropins was not affected by ALAN during the period of the experiments. Thus, suspected implications of ALAN on reproduction of roach could not be substantiated.

  8. Otter ( Lutra lutra ) predation on stocked brown trout ( Salmo trutta ) in two Danish lowland rivers

    DEFF Research Database (Denmark)

    Jacobsen, Lene

    2005-01-01

    This study aimed to evaluate otter predation on stocked trout. Large hatchery-reared trout (16-30 cm) were stocked into two Danish rivers with different fish populations. Otter diet before and after trout stocking was determined by analysing 685 spraints, collected regularly during the 35-day study...... period. Fish composition in the rivers before stocking was assessed by electrofishing. In River Trend, a typical trout river, the proportion of trout in the otter diet increased from 8% before stocking to 33% a few days after stocking. Moreover, trout lengths in the diet changed significantly towards...... the lengths of stocked trout, indicating that newly stocked trout were preferred to wild trout. In River Skals, dominated by cyprinids, there was no change in otter diet after stocking of hatchery trout, i.e., these were ignored by otter. Otter predation should be taken into account together with fish...

  9. The freshwater grass shrimp Palaemonetes antennarius in the diet of fish in Lake Bracciano (Central Italy

    Directory of Open Access Journals (Sweden)

    Traversetti L.

    2016-01-01

    Full Text Available We evaluated the importance of the freshwater grass shrimp Palaemonetes antennarius as trophic source for the lacustrine fish of the Lake Bracciano (Central Italy analyzing 6120 stomach contents of 10 species. Shrimp was recorded for all size classes of the investigated species with a variation in frequency and abundance depending on seasons. P. antennarius was occasionally preyed by cyprinids, and systematically by small individuals of carnivorous fish. Our findings acquire more importance if we take the shrimps ecological niche into account, as it is potentially acting as detritivorous/generalist and predator of benthic invertebrates as well. This feeding behavior makes P. antennarius an important network ring, being a taxon that ought to receive the same attention recommended for other freshwater decapods as proposed for Austropotamobius pallipes and Potamon fluviatile.

  10. Preliminary aspects concerning phytoplankton structure in the Balta Mare – Carja 1 fish farm

    Directory of Open Access Journals (Sweden)

    Maria FETECAU

    2010-08-01

    Full Text Available The present paper presents information on the structure and the dynamics of the water ecosystem’s phytoplankton of the Carja 1 fish farm - Vaslui County, carps and Asian cyprinids are grown ascommon fish. To establish the structure and the dynamics of the phytoplankton, two samples from 6 stations were taken in the spring and in the autumn, using of a Garmin GPS 7- type navigation system.When analysing the number of individuals and the algae species present in the phytoplankton’s structure, one can notice the low development level of the vegetable plankton. From the quantity point of view, one can notice the numerical abundance of the clorophyceae in all the analysed samples. The dominant species were: Scenedesmus acuminatus, Scenedesmus quadricauda, Tetrastrum staurogenieforme. The small number of species and algae specimens determined in the phytoplankton’s structure emphasizes the reduced level of trophicity and biodiversity of the analysed ecosystem.

  11. Socio-economic drivers of specialist anglers targeting the non-native European catfish (Silurus glanis in the UK.

    Directory of Open Access Journals (Sweden)

    E M Ann Rees

    Full Text Available Information about the socioeconomic drivers of Silurus glanis anglers in the UK were collected using questionnaires from a cross section of mixed cyprinid fisheries to elucidate human dimensions in angling and non-native fisheries management. Respondents were predominantly male (95%, 30-40 years of age with £500 per annum. The proportion of time spent angling for S. glanis was significantly related to angler motivations; fish size, challenge in catch, tranquil natural surroundings, escape from daily stress and to be alone were considered important drivers of increased time spent angling. Overall, poor awareness of: the risks and adverse ecological impacts associated with introduced S. glanis, non-native fisheries legislation, problems in use of unlimited ground bait and high fish stocking rates in angling lakes were evident, possibly related to inadequate training and information provided by angling organisations to anglers, as many stated that they were insufficiently informed.

  12. Importance of small fishes and invasive crayfish in otter Lutra lutra diet in an English chalk stream

    Directory of Open Access Journals (Sweden)

    Britton J. Robert

    2017-01-01

    Full Text Available The diet composition of the European otter Lutra lutra was assessed using spraint analysis in the Hampshire Avon, a lowland chalk stream in Southern England, over an 18-month period. Small cyprinid fishes were the main prey item taken in all seasons, with bullhead Cottus gobio and stone loach Barbatula barbatula also important; there were relatively few larger fishes of interest to fisheries found. There were significant seasonal differences in diet composition by season, with signal crayfish Pacifastacus leniusculus only being prominent prey items in warmer months and amphibians in winter, revealing that non-fish resources were seasonally important dietary components. Reconstructed body lengths of prey revealed the only species present in diet >350 mm was pike Esox lucius. These dietary data thus provide important information for informing conservation conflicts between otters and fishery interests.

  13. Occurrence of Deoxynivalenol and Zearalenone in Commercial Fish Feed: An Initial Study

    Directory of Open Access Journals (Sweden)

    Sven Dänicke

    2013-01-01

    Full Text Available The control of mycotoxins is a global challenge not only in human consumption but also in nutrition of farm animals including aquatic species. Fusarium toxins, such as deoxynivalenol (DON and zearalenone (ZEN, are common contaminants of animal feed but no study reported the occurrence of both mycotoxins in fish feed so far. Here, we report for the first time the occurrence of DON and ZEN in samples of commercial fish feed designed for nutrition of cyprinids collected from central Europe. A maximal DON concentration of 825 μg kg−1 feed was found in one feed whereas average values of 289 μg kg−1 feed were noted. ZEN was the more prevalent mycotoxin but the concentrations were lower showing an average level of 67.9 μg kg−1 feed.

  14. Herpesviruses that Infect Fish

    Directory of Open Access Journals (Sweden)

    Moshe Kotler

    2011-11-01

    Full Text Available Herpesviruses are host specific pathogens that are widespread among vertebrates. Genome sequence data demonstrate that most herpesviruses of fish and amphibians are grouped together (family Alloherpesviridae and are distantly related to herpesviruses of reptiles, birds and mammals (family Herpesviridae. Yet, many of the biological processes of members of the order Herpesvirales are similar. Among the conserved characteristics are the virion structure, replication process, the ability to establish long term latency and the manipulation of the host immune response. Many of the similar processes may be due to convergent evolution. This overview of identified herpesviruses of fish discusses the diseases that alloherpesviruses cause, the biology of these viruses and the host-pathogen interactions. Much of our knowledge on the biology of Alloherpesvirdae is derived from research with two species: Ictalurid herpesvirus 1 (channel catfish virus and Cyprinid herpesvirus 3 (koi herpesvirus.

  15. Herpesviruses that infect fish.

    Science.gov (United States)

    Hanson, Larry; Dishon, Arnon; Kotler, Moshe

    2011-11-01

    Herpesviruses are host specific pathogens that are widespread among vertebrates. Genome sequence data demonstrate that most herpesviruses of fish and amphibians are grouped together (family Alloherpesviridae) and are distantly related to herpesviruses of reptiles, birds and mammals (family Herpesviridae). Yet, many of the biological processes of members of the order Herpesvirales are similar. Among the conserved characteristics are the virion structure, replication process, the ability to establish long term latency and the manipulation of the host immune response. Many of the similar processes may be due to convergent evolution. This overview of identified herpesviruses of fish discusses the diseases that alloherpesviruses cause, the biology of these viruses and the host-pathogen interactions. Much of our knowledge on the biology of Alloherpesvirdae is derived from research with two species: Ictalurid herpesvirus 1 (channel catfish virus) and Cyprinid herpesvirus 3 (koi herpesvirus).

  16. Complete genome sequence and architecture of crucian carp Carassius auratus herpesvirus (CaHV).

    Science.gov (United States)

    Zeng, Xiao-Tao; Chen, Zhong-Yuan; Deng, Yuan-Sheng; Gui, Jian-Fang; Zhang, Qi-Ya

    2016-12-01

    Crucian carp Carassius auratus herpesvirus (CaHV) was isolated from diseased crucian carp with acute gill hemorrhages and high mortality. The CaHV genome was sequenced and analyzed. The data showed that it consists of 275,348 bp and contains 150 predicted ORFs. The architecture of the CaHV genome differs from those of four cyprinid herpesviruses (CyHV1, CyHV2, SY-C1, CyHV3), with insertions, deletions and the absence of a terminal direct repeat. Phylogenetic analysis of the DNA polymerase sequences of 17 strains of Herpesvirales members, and the concatenated 12 core ORFs from 10 strains of alloherpesviruses showed that CaHV clustered together with members of the genus Cyprinivirus, family Alloherpesviridae.

  17. Herpesviruses that Infect Fish

    Science.gov (United States)

    Hanson, Larry; Dishon, Arnon; Kotler, Moshe

    2011-01-01

    Herpesviruses are host specific pathogens that are widespread among vertebrates. Genome sequence data demonstrate that most herpesviruses of fish and amphibians are grouped together (family Alloherpesviridae) and are distantly related to herpesviruses of reptiles, birds and mammals (family Herpesviridae). Yet, many of the biological processes of members of the order Herpesvirales are similar. Among the conserved characteristics are the virion structure, replication process, the ability to establish long term latency and the manipulation of the host immune response. Many of the similar processes may be due to convergent evolution. This overview of identified herpesviruses of fish discusses the diseases that alloherpesviruses cause, the biology of these viruses and the host-pathogen interactions. Much of our knowledge on the biology of Alloherpesvirdae is derived from research with two species: Ictalurid herpesvirus 1 (channel catfish virus) and Cyprinid herpesvirus 3 (koi herpesvirus). PMID:22163339

  18. Immunological relatedness of gonadotrophins of various fishes as shown by radioimmunoassays

    International Nuclear Information System (INIS)

    Tan, E.S.P.; Dodd, J.M.

    1978-01-01

    Pituitary extracts and plasmas of 35 species of fish were tested in two radioimmunoassay (RIA) systems, a salmon-salmon homologous RIA and a salmon-carp heterologous RIA, in which the same antiserum, raised against salmon gonadotrophin, SG-G100, was employed. In the homologous RIA, most salmonid species tested, except for the powan and ayu, cross-reacted in a manner identical with that of the standard, SG-DEAE-3. Nonparallelism of inhibition curves were found in 13 non- salmonid species while 3 others showed non cross-reaction. In the heterologous RIA, all cyprinids, except the rudd, and all salmonids, except the ayu, as well as 9 other species, gave inhibition curves parallel to that of the standard purified carp gonadotrophin. These results may indicate that immunological properties of fish gonadotrophins do not correspond to known phylogenetic relationships of fishes

  19. Fish diversity in adjacent ambient, thermal, and post-thermal freshwater streams

    International Nuclear Information System (INIS)

    McFarlane, R.W.

    1976-01-01

    The Savannah River Plant area is drained by five streams of various sizes and thermal histories. One has never been thermally stressed, two presently receive thermal effluent, and two formerly received thermal effluent from nuclear production reactors. Sixty-four species of fishes are known to inhabit these streams; 55 species is the highest number obtained from any one stream. Thermal effluent in small streams excludes fish during periods of high temperatures, but the streams are rapidly reinvaded when temperatures subside below lethal limits. Some cyprinids become extinct in nonthermal tributaries upstream from the thermal effluents after extended periods of thermal stress. This extinction is similar to that which follows stream impoundment. Post-thermal streams rapidly recover their fish diversity and abundance. The alteration of the streambed and removal of overhead canopy may change the stream characteristics and modify the post-thermal fish fauna

  20. Genome sequence and genetic diversity of the common carp, Cyprinus carpio.

    Science.gov (United States)

    Xu, Peng; Zhang, Xiaofeng; Wang, Xumin; Li, Jiongtang; Liu, Guiming; Kuang, Youyi; Xu, Jian; Zheng, Xianhu; Ren, Lufeng; Wang, Guoliang; Zhang, Yan; Huo, Linhe; Zhao, Zixia; Cao, Dingchen; Lu, Cuiyun; Li, Chao; Zhou, Yi; Liu, Zhanjiang; Fan, Zhonghua; Shan, Guangle; Li, Xingang; Wu, Shuangxiu; Song, Lipu; Hou, Guangyuan; Jiang, Yanliang; Jeney, Zsigmond; Yu, Dan; Wang, Li; Shao, Changjun; Song, Lai; Sun, Jing; Ji, Peifeng; Wang, Jian; Li, Qiang; Xu, Liming; Sun, Fanyue; Feng, Jianxin; Wang, Chenghui; Wang, Shaolin; Wang, Baosen; Li, Yan; Zhu, Yaping; Xue, Wei; Zhao, Lan; Wang, Jintu; Gu, Ying; Lv, Weihua; Wu, Kejing; Xiao, Jingfa; Wu, Jiayan; Zhang, Zhang; Yu, Jun; Sun, Xiaowen

    2014-11-01

    The common carp, Cyprinus carpio, is one of the most important cyprinid species and globally accounts for 10% of freshwater aquaculture production. Here we present a draft genome of domesticated C. carpio (strain Songpu), whose current assembly contains 52,610 protein-coding genes and approximately 92.3% coverage of its paleotetraploidized genome (2n = 100). The latest round of whole-genome duplication has been estimated to have occurred approximately 8.2 million years ago. Genome resequencing of 33 representative individuals from worldwide populations demonstrates a single origin for C. carpio in 2 subspecies (C. carpio Haematopterus and C. carpio carpio). Integrative genomic and transcriptomic analyses were used to identify loci potentially associated with traits including scaling patterns and skin color. In combination with the high-resolution genetic map, the draft genome paves the way for better molecular studies and improved genome-assisted breeding of C. carpio and other closely related species.

  1. Evidence for fish dispersal from spatial analysis of stream network topology

    Science.gov (United States)

    Hitt, N.P.; Angermeier, P.L.

    2008-01-01

    Developing spatially explicit conservation strategies for stream fishes requires an understanding of the spatial structure of dispersal within stream networks. We explored spatial patterns of stream fish dispersal by evaluating how the size and proximity of connected streams (i.e., stream network topology) explained variation in fish assemblage structure and how this relationship varied with local stream size. We used data from the US Environmental Protection Agency's Environmental Monitoring and Assessment Program in wadeable streams of the Mid-Atlantic Highlands region (n = 308 sites). We quantified stream network topology with a continuous analysis based on the rate of downstream flow accumulation from sites and with a discrete analysis based on the presence of mainstem river confluences (i.e., basin area >250 km2) within 20 fluvial km (fkm) from sites. Continuous variation in stream network topology was related to local species richness within a distance of ???10 fkm, suggesting an influence of fish dispersal within this spatial grain. This effect was explained largely by catostomid species, cyprinid species, and riverine species, but was not explained by zoogeographic regions, ecoregions, sampling period, or spatial autocorrelation. Sites near mainstem river confluences supported greater species richness and abundance of catostomid, cyprinid, and ictalurid fishes than did sites >20 fkm from such confluences. Assemblages at sites on the smallest streams were not related to stream network topology, consistent with the hypothesis that local stream size regulates the influence of regional dispersal. These results demonstrate that the size and proximity of connected streams influence the spatial distribution of fish and suggest that these influences can be incorporated into the designs of stream bioassessments and reserves to enhance management efficacy. ?? 2008 by The North American Benthological Society.

  2. THE RESEARCH IN FISH GENETICS IN CROATIA AND FORMER YUGOSLAVIA

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    Tomislav Treer

    1994-03-01

    Full Text Available This is a review on fish genetics research in Croatia and former Yugoslavia, based on the analyses of all the articles published in four main journals (Ribarstvo Jugoslavije, Morsko ribarstvo, Ichthyologia and Acta Adriatica since 1945 till disintegration of Yugoslavia in 1991. Most of the papers cover the fields on cytogenetics and hybridization (24 and 13 respectively. Eight papers were on fish selection and five on population genetics. Apart from those, five papers were written by foreign authors. Two groups of researchers from the University of Sarajevo were specially active. One of them lead by B e r b e r o v i ć and S o f r a d ž i j a did extensive work in cytogenetics, analyzing the karyotypes of many fish species, some of them endemic. Another one lead by V u k o v i ć , investigated some natural hybrids and created many of them artificially, particulary among cyprinids. These results are presented in a special table. Contrary to the mountainous Bosnia where this type of research was of systematic and ecologic importance, in Croatia whwrw aquaculture was highly developed, the approach was quite different. The scientists from the University of Zagreb, H a b e k o v i ć and T u r k , studied the hybridization and selection of important cultured cyprinids. Apart from these scientific groups, many papers were published by A l - S a b t i , who later became world famous in fish cytogenetics. The works of many other authors who contributed with papers in different fields of fish genetics are also described.

  3. New host record and molecular characterization of Dicauda atherinoidi Hoffman & Walker (Bivalvulida: Myxobolidae): a parasite of the emerald shiner Notropis atherinoides Rafinesque, 1818 and mimic shiner Notropis vollucellus Cope, 1865.

    Science.gov (United States)

    Loch, T P; Rosser, T G; Baumgartner, W A; Boontai, T; Faisal, M; Griffin, M J

    2017-10-01

    Updated morphological and histopathological descriptions for Dicauda atherinoidi (Bivalvulida:Myxobolidae) and an expanded host range are supplemented with the first molecular data and phylogenetic analyses of the genus. Plasmodia were located on the head, ventrum/body and fins of infected emerald shiner Notropis atherinoides Rafinesque, 1818 and mimic shiner Notropis vollucellus Cope, 1865, a new host species. Myxospores were spherical, ranging 9.3-11.4 μm (10.5 ± 0.4) in length, 9.0-11.0 μm (9.7 ± 0.4) in width and 6.6-7.0 μm (6.8 ± 0.2) thick in sutural view, and possessed 2-3 caudal processes (5.3-68.3 μm, 31.1 ± 13.6) connected to the spore body at the sutural groove, all of which are consistent with the genus Dicauda. In the absence of available Dicauda sequence data, the 18S rDNA sequences from Michigan isolates were most similar to Myxobolus spp. Phylogenetic analyses clustered these isolates with myxobolid species from cyprinid fish, suggesting these parasites may represent an underpopulated group of cyprinid-infecting myxozoans. Histopathology revealed thin-walled plasmodial pseudocysts in the dermis and associated connective tissue, where granulomatous inflammation and focal scale atrophy were also present. Further sampling/sequencing of myxobolids from Notropis spp. should expand these underrepresented myxozoans and offer further insight into Myxobolidae host family tropisms. © 2017 John Wiley & Sons Ltd.

  4. An electron microscopic investigation into the possible source of new muscle fibres in teleost fish.

    Science.gov (United States)

    Stoiber, W; Sänger, A M

    1996-12-01

    This study is based on transmission electron microscopic (TEM) investigations of deep (fast, 'white') teleost fish muscle proliferation in early developmental stages of three European cyprinid species and the rainbow trout. Our fine structural findings provide evidence that early myotomal growth in these animals may utilize different mechanisms that are activated in close succession during early life history. First, initial enlargement of the deep muscle bulk in the embryo seems to be due to hypertrophy of the somite-cell derived stock of muscle fibres. Second, we suggest that deep muscle growth becomes additionally powered by attachment of presumptive myogenic cells that originate from and proliferate within the adjacent mesenchymal tissue lining. Third, mesenchyme-derived muscle cell precursors are thought to enter the myotomes via the myosepta. After migration between the pre-established muscle fibres these cells may function as myosatellite cells, thus at least partly providing the stem cell population for subsequent rapid hyperplastic growth. Finally, there is evidence that presumptive deep muscle satellite cells also proliferate by mitotic division in situ. A similar process of myogenic cell migration and proliferation may foster intermediate fibre differentiation. The model of myogenic cell migration is discussed in view of in vitro and in vivo data on satellite cell migratory power and with respect to temperature-induced and species dependent differences. As for the latter, our results indicate that patterns of muscle differentiation may diverge between a fast growing salmonid species and a moderately growing cyprinid species of similar final size. The model is compatible with the well-established idea that teleost muscle growth may rely on different subclasses of myosatellite cells.

  5. Fauna parasitária de peixes oriundos de “pesque-pague” do município de Guariba, São Paulo, Brasil - DOI: 10.4025/actascibiolsci.v28i3.253 Parasitic fauna of cultivated fishes in fee fishing farm of Guariba, São Paulo State, Brazil - DOI: 10.4025/actascibiolsci.v28i3.253

    Directory of Open Access Journals (Sweden)

    Julieta Rodini Engracia de Moraes

    2007-11-01

    Full Text Available Este trabalho descreve a ocorrência e a sazonalidade de parasitos em peixes de “pesque-pague” do município de Guariba, Estado de São Paulo, Brasil (21º15`22``S, 48º18`58``W e 595 m de altitude, durante o período de agosto de 2001 a julho de 2002. A presença de parasitos foi pesquisada em pacu Piaractus mesopotamicus (Characidae, carpa comum Cyprinus carpio (Cyprinidae, tilápia-do-Nilo Oreochromis niloticus (Cichlidae, híbrido tambacu (macho de P. mesopotamicus x fêmea de tambaqui-Colossoma macropomum e piraputanga Brycon hillari (Characidae. Os resultados demonstram que dos 100 peixes examinados, 15% estavam parasitados por pelo menos um dos seguintes parasitos: Trichodina sp.; helmintos monogenóides; copepoditos de Lernaea cyprinacea; L. cyprinacea adulta ou Dolops carvalhoi. Por ordem decrescente, o grau de suscetibilidade dos hospedeiros foi C. carpio, P. mesopotamicus, B. hillari, híbrido tambacu e O. niloticus. Por ordem decrescente, os parasitos encontrados foram helmintos monogenóides, Dolops carvalhoi, Trichodina sp., Lernaea cyprinacea adultas e suas formas jovens.This study describes the occurrence and the seasonality of parasites of cultivated fish from a fee fishing farm located in Guariba, São Paulo State, Brazil (21º15`22`` S, 48º18`58`` W and 595 m of altitude, from August, 2001 to July, 2002. The presence of parasites was researched in pacu Piaractus mesopotamicus (Characidae, common carp Cyprinus carpio (Cyprinidae, nile-tilapia Oreochromis niloticus (Cichlidae, tambacu hybrid (male of P. mesopotamicus x female of tambaqui-Colossoma macropomum and piraputanga Brycon hillari (Characidae. Results demonstrate that out of 100 fish examined, 15% were sponged for at least one of the following parasites: Trichodina sp.; monogenean helminths; copepodits of Lernaea cyprinacea; adults of L. cyprinacea; or Dolops carvalhoi. In decreasing order, the susceptibility degree of the hosts was C. carpio, P. mesopotamicus, B

  6. DNA barcoding commercially important fish species of Turkey.

    Science.gov (United States)

    Keskın, Emre; Atar, Hasan H

    2013-09-01

    DNA barcoding was used in the identification of 89 commercially important freshwater and marine fish species found in Turkish ichthyofauna. A total of 1765 DNA barcodes using a 654-bp-long fragment of the mitochondrial cytochrome c oxidase subunit I gene were generated for 89 commercially important freshwater and marine fish species found in Turkish ichthyofauna. These species belong to 70 genera, 40 families and 19 orders from class Actinopterygii, and all were associated with a distinct DNA barcode. Nine and 12 of the COI barcode clusters represent the first species records submitted to the BOLD and GenBank databases, respectively. All COI barcodes (except sequences of first species records) were matched with reference sequences of expected species, according to morphological identification. Average nucleotide frequencies of the data set were calculated as T = 29.7%, C = 28.2%, A = 23.6% and G = 18.6%. Average pairwise genetic distance among individuals were estimated as 0.32%, 9.62%, 17,90% and 22.40% for conspecific, congeneric, confamilial and within order, respectively. Kimura 2-parameter genetic distance values were found to increase with taxonomic level. For most of the species analysed in our data set, there is a barcoding gap, and an overlap in the barcoding gap exists for only two genera. Neighbour-joining trees were drawn based on DNA barcodes and all the specimens clustered in agreement with their taxonomic classification at species level. Results of this study supported DNA barcoding as an efficient molecular tool for a better monitoring, conservation and management of fisheries. © 2013 John Wiley & Sons Ltd.

  7. The GC-heterogeneity of teleost fishes

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    Gautier Christian

    2008-12-01

    Full Text Available Abstract Background One of the most striking features of mammalian and birds chromosomes is the variation in the guanine-cytosine (GC content that occurs over scales of hundreds of kilobases to megabases; this is known as the "isochore" structure. Among other vertebrates the presence of isochores depends upon the taxon; isochore are clearly present in Crocodiles and turtles but fish genome seems very homogeneous on GC content. This has suggested a unique isochore origin after the divergence between Sarcopterygii and Actinopterygii, but before that between Sauropsida and mammals. However during more than 30 years of analysis, isochore characteristics have been studied and many important biological properties have been associated with the isochore structure of human genomes. For instance, the genes are more compact and their density is highest in GC rich isochores. Results This paper shows in teleost fish genomes the existence of "GC segmentation" sharing some of the characteristics of isochores although teleost fish genomes presenting a particular homogeneity in CG content. The entire genomes of T nigroviridis and D rerio are now available, and this has made it possible to check whether a mosaic structure associated with isochore properties can be found in these fishes. In this study, hidden Markov models were trained on fish genes (T nigroviridis and D rerio which were classified by using the isochore class of their human orthologous. A clear segmentation of these genomes was detected. Conclusion The GC content is an excellent indicator of isochores in heterogeneous genomes as mammals. The segmentation we obtained were well correlated with GC content and other properties associated to GC content such as gene density, the number of exons per gene and the length of introns. Therefore, the GC content is the main property that allows the detection of isochore but more biological properties have to be taken into account. This method allows detecting

  8. Updated review of marine alien species and other ‘newcomers’ recorded from the Maltese Islands (Central Mediterranean

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    J. EVANS

    2015-03-01

    Full Text Available An updated review of marine alien species and other ‘newcomers’ recorded from the Maltese Islands is presented on account of new records and amendments to a previous review in 2007. Species were classified according to their establishment status (‘Questionable’, ‘Casual’, ‘Established’, ‘Invasive’ and origin (‘Alien’, ‘Range expansion’, ‘Cryptogenic’. A total of 31 species were added to the inventory, while 6 species have been removed, bringing the total number of species to 73. Of these, 66 are considered to be aliens (or putative aliens but with uncertain origin with the remaining 7 resulting from range expansion. Six records are considered to be questionable and hence unverified. For verified records, the dominant taxonomic groups are Mollusca (represented by 21 species and Actinopterygii (15 species, followed by Crustacea (8 species and Rhodophyta (7 species. Eight of these species (aliens: Caulerpa cylindracea, Lophocladia lallemandi, Womersleyella setacea, Brachidontes pharaonis, Percnon gibbesi, Fistularia commersonii, Siganus luridus; range extender: Sphoeroides pachygaster are considered to be invasive. The introduction pathway for 30 species is unknown. Amongst the alien species, ‘Shipping’ is the most common introduction pathway, followed by ‘Secondary dispersal’ from elsewhere in the Mediterranean Sea. An increasing trend in the number of alien marine species reported from the Maltese Islands is evident, with a peak of 22 species recorded during the last decade (2001–2010. A discussion on the rationale for including range-expanding species in national inventories of recent arrivals, and in the analysis of trends in records from the Maltese Islands, is included. In particular, the general warming trend of Mediterranean surface waters appears to be facilitating the westward spread of thermophilic alien species from the Eastern to the Central Mediterranean, and the eastward range expansion of

  9. An update on MyoD evolution in teleosts and a proposed consensus nomenclature to accommodate the tetraploidization of different vertebrate genomes.

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    Daniel J Macqueen

    Full Text Available BACKGROUND: MyoD is a muscle specific transcription factor that is essential for vertebrate myogenesis. In several teleost species, including representatives of the Salmonidae and Acanthopterygii, but not zebrafish, two or more MyoD paralogues are conserved that are thought to have arisen from distinct, possibly lineage-specific duplication events. Additionally, two MyoD paralogues have been characterised in the allotetraploid frog, Xenopus laevis. This has lead to a confusing nomenclature since MyoD paralogues have been named outside of an appropriate phylogenetic framework. METHODS AND PRINCIPAL FINDINGS: Here we initially show that directly depicting the evolutionary relationships of teleost MyoD orthologues and paralogues is hindered by the asymmetric evolutionary rate of Acanthopterygian MyoD2 relative to other MyoD proteins. Thus our aim was to confidently position the event from which teleost paralogues arose in different lineages by a comparative investigation of genes neighbouring myod across the vertebrates. To this end, we show that genes on the single myod-containing chromosome of mammals and birds are retained in both zebrafish and Acanthopterygian teleosts in a striking pattern of double conserved synteny. Further, phylogenetic reconstruction of these neighbouring genes using Bayesian and maximum likelihood methods supported a common origin for teleost paralogues following the split of the Actinopterygii and Sarcopterygii. CONCLUSION: Our results strongly suggest that myod was duplicated during the basal teleost whole genome duplication event, but was subsequently lost in the Ostariophysi (zebrafish and Protacanthopterygii lineages. We propose a sensible consensus nomenclature for vertebrate myod genes that accommodates polyploidization events in teleost and tetrapod lineages and is justified from a phylogenetic perspective.

  10. Hox gene clusters in the Indonesian coelacanth, Latimeria menadoensis

    Science.gov (United States)

    Koh, Esther G. L.; Lam, Kevin; Christoffels, Alan; Erdmann, Mark V.; Brenner, Sydney; Venkatesh, Byrappa

    2003-01-01

    The Hox genes encode transcription factors that play a key role in specifying body plans of metazoans. They are organized into clusters that contain up to 13 paralogue group members. The complex morphology of vertebrates has been attributed to the duplication of Hox clusters during vertebrate evolution. In contrast to the single Hox cluster in the amphioxus (Branchiostoma floridae), an invertebrate-chordate, mammals have four clusters containing 39 Hox genes. Ray-finned fishes (Actinopterygii) such as zebrafish and fugu possess more than four Hox clusters. The coelacanth occupies a basal phylogenetic position among lobe-finned fishes (Sarcopterygii), which gave rise to the tetrapod lineage. The lobe fins of sarcopterygians are considered to be the evolutionary precursors of tetrapod limbs. Thus, the characterization of Hox genes in the coelacanth should provide insights into the origin of tetrapod limbs. We have cloned the complete second exon of 33 Hox genes from the Indonesian coelacanth, Latimeria menadoensis, by extensive PCR survey and genome walking. Phylogenetic analysis shows that 32 of these genes have orthologs in the four mammalian HOX clusters, including three genes (HoxA6, D1, and D8) that are absent in ray-finned fishes. The remaining coelacanth gene is an ortholog of hoxc1 found in zebrafish but absent in mammals. Our results suggest that coelacanths have four Hox clusters bearing a gene complement more similar to mammals than to ray-finned fishes, but with an additional gene, HoxC1, which has been lost during the evolution of mammals from lobe-finned fishes. PMID:12547909

  11. Evolution of the branchiostegal membrane and restricted gill openings in Actinopterygian fishes.

    Science.gov (United States)

    Farina, Stacy C; Near, Thomas J; Bemis, William E

    2015-06-01

    A phylogenetic survey is a powerful approach for investigating the evolutionary history of a morphological characteristic that has evolved numerous times without obvious functional implications. Restricted gill openings, an extreme modification of the branchiostegal membrane, are an example of such a characteristic. We examine the evolution of branchiostegal membrane morphology and highlight convergent evolution of restricted gill openings. We surveyed specimens from 433 families of actinopterygians for branchiostegal membrane morphology and measured head and body dimensions. We inferred a relaxed molecular clock phylogeny with branch length estimates based on nine nuclear genes sampled from 285 species that include all major lineages of Actinopterygii. We calculated marginal state reconstructions of four branchiostegal membrane conditions and found that restricted gill openings have evolved independently in at least 11 major actinopterygian clades, and the total number of independent origins of the trait is likely much higher. A principal component analysis revealed that fishes with restricted gill openings occupy a larger morphospace, as defined by our linear measurements, than do fishes with nonrestricted openings. We used a decision tree analysis of ecological data to determine if restricted gill openings are linked to certain environments. We found that fishes with restricted gill openings repeatedly occur under a variety of ecological conditions, although they are rare in open-ocean pelagic environments. We also tested seven ratios for their utility in distinguishing between fishes with and without restricted gill openings, and we propose a simple metric for quantifying restricted gill openings (RGO), defined as a ratio of the distance from the ventral midline to the gill opening relative to half the circumference of the head. Functional explanations for this specialized morphology likely differ within each clade, but its repeated evolution indicates a need

  12. An in vivo analysis of Cr6+ induced biochemical, genotoxicological and transcriptional profiling of genes related to oxidative stress, DNA damage and apoptosis in liver of fish, Channa punctatus (Bloch, 1793).

    Science.gov (United States)

    Awasthi, Yashika; Ratn, Arun; Prasad, Rajesh; Kumar, Manoj; Trivedi, Sunil P

    2018-07-01

    Present study was designed to assess the hexavalent chromium (Cr 6+ ) mediated oxidative stress that induces DNA damage and apoptosis in adult fish, Channa punctatus (35 ± 3.0 g; 14.5 ± 1.0 cm; Actinopterygii). Fishes were maintained in three groups for 15, 30 and 45 d of exposure periods. They were treated with 5% (Group T1) and 10% (Group T2) of 96 h-LC 50 of chromium trioxide (Cr 6+ ). Controls were run for the similar duration. A significant (p < 0.05) increment in the activities of antioxidant enzymes, SOD and CAT in liver tissues of the exposed fish evinces the persistence of oxidative stress. A significant (p < 0.05) increase in induction of micronuclei (MN) coupled with transcriptional responses of target genes related to antioxidant enzymes, DNA damage and apoptosis (sod, cat, gsr, nox-1, p53, bax, bcl-2, apaf-1 and casp3a) establishes the impact of oxidative stress due to in vivo, Cr 6+ accumulation in liver as compared to control (0 mg/L), in a dose and exposure-dependent manner. Initially, the increased level of reactive oxygen species (ROS) in liver coincided with that of enhanced mRNA expression of antioxidant enzymes, sod, cat, gsr and nox-1 but, later, the overproduction of ROS, after 45 d of exposure of Cr 6+ , resulted in a significant (p < 0.05) up-regulation of p53. Our findings also unveil that the up-regulation of bax, apaf-1 and casp3a and down-regulation of bcl-2 are associated with Cr 6+ -induced oxidative stress mediated-apoptosis in liver of test fish. Aforesaid molecular markers can, thus, be efficiently utilized for bio-monitoring of aquatic regimes and conservation of fish biodiversity. Copyright © 2018 Elsevier B.V. All rights reserved.

  13. Determining the diet of larvae of western rock lobster (Panulirus cygnus using high-throughput DNA sequencing techniques.

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    Richard O'Rorke

    Full Text Available The Western Australian rock lobster fishery has been both a highly productive and sustainable fishery. However, a recent dramatic and unexplained decline in post-larval recruitment threatens this sustainability. Our lack of knowledge of key processes in lobster larval ecology, such as their position in the food web, limits our ability to determine what underpins this decline. The present study uses a high-throughput amplicon sequencing approach on DNA obtained from the hepatopancreas of larvae to discover significant prey items. Two short regions of the 18S rRNA gene were amplified under the presence of lobster specific PNA to prevent lobster amplification and to improve prey amplification. In the resulting sequences either little prey was recovered, indicating that the larval gut was empty, or there was a high number of reads originating from multiple zooplankton taxa. The most abundant reads included colonial Radiolaria, Thaliacea, Actinopterygii, Hydrozoa and Sagittoidea, which supports the hypothesis that the larvae feed on multiple groups of mostly transparent gelatinous zooplankton. This hypothesis has prevailed as it has been tentatively inferred from the physiology of larvae, captive feeding trials and co-occurrence in situ. However, these prey have not been observed in the larval gut as traditional microscopic techniques cannot discern between transparent and gelatinous prey items in the gut. High-throughput amplicon sequencing of gut DNA has enabled us to classify these otherwise undetectable prey. The dominance of the colonial radiolarians among the gut contents is intriguing in that this group has been historically difficult to quantify in the water column, which may explain why they have not been connected to larval diet previously. Our results indicate that a PCR based technique is a very successful approach to identify the most abundant taxa in the natural diet of lobster larvae.

  14. Feed intake and brain neuropeptide Y (NPY) and cholecystokinin (CCK) gene expression in juvenile cobia fed plant-based protein diets with different lysine to arginine ratios.

    Science.gov (United States)

    Nguyen, Minh Van; Jordal, Ann-Elise Olderbakk; Espe, Marit; Buttle, Louise; Lai, Hung Van; Rønnestad, Ivar

    2013-07-01

    Cobia (Rachycentron canadum, Actinopterygii, Perciformes;10.5±0.1g) were fed to satiation with three plant-based protein test diets with different lysine (L) to arginine (A) ratios (LL/A, 0.8; BL/A, 1.1; and HL/A, 1.8), using a commercial diet as control for six weeks. The test diets contained 730 g kg(-1) plant ingredients with 505-529 g protein, 90.2-93.9 g lipid kg(-1) dry matter; control diet contained 550 g protein and 95 g lipid kg(-1) dry matter. Periprandial expression of brain NPY and CCK (npy and cck) was measured twice (weeks 1 and 6). At week one, npy levels were higher in pre-feeding than postfeeding cobia for all diets, except LL/A. At week six, npy levels in pre-feeding were higher than in postfeeding cobia for all diets. cck in pre-feeding cobia did not differ from that in postfeeding for all diets, at either time point. Cobia fed LL/A had lower feed intake (FI) than cobia fed BL/A and control diet, but no clear correlations between dietary L/A ratio and FI, growth and expression of npy and cck were detected. The data suggest that NPY serves as an orexigenic factor, but further studies are necessary to describe links between dietary L/A and regulation of appetite and FI in cobia. Copyright © 2013 Elsevier Inc. All rights reserved.

  15. A Bayesian framework to estimate diversification rates and their variation through time and space

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    Silvestro Daniele

    2011-10-01

    Full Text Available Abstract Background Patterns of species diversity are the result of speciation and extinction processes, and molecular phylogenetic data can provide valuable information to derive their variability through time and across clades. Bayesian Markov chain Monte Carlo methods offer a promising framework to incorporate phylogenetic uncertainty when estimating rates of diversification. Results We introduce a new approach to estimate diversification rates in a Bayesian framework over a distribution of trees under various constant and variable rate birth-death and pure-birth models, and test it on simulated phylogenies. Furthermore, speciation and extinction rates and their posterior credibility intervals can be estimated while accounting for non-random taxon sampling. The framework is particularly suitable for hypothesis testing using Bayes factors, as we demonstrate analyzing dated phylogenies of Chondrostoma (Cyprinidae and Lupinus (Fabaceae. In addition, we develop a model that extends the rate estimation to a meta-analysis framework in which different data sets are combined in a single analysis to detect general temporal and spatial trends in diversification. Conclusions Our approach provides a flexible framework for the estimation of diversification parameters and hypothesis testing while simultaneously accounting for uncertainties in the divergence times and incomplete taxon sampling.

  16. On the possibility of using biological toxicity tests to monitor the work of wastewater treatment plants

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    Zorić Jelena

    2008-01-01

    Full Text Available The aim of this study was to ascertain the possibility of using biological toxicity tests to monitor influent and effluent wastewaters of wastewater treatment plants. The information obtained through these tests is used to prevent toxic pollutants from entering wastewater treatment plants and discharge of toxic pollutants into the recipient. Samples of wastewaters from the wastewater treatment plants of Kragujevac and Gornji Milanovac, as well as from the Lepenica and Despotovica Rivers immediately before and after the influx of wastewaters from the plants, were collected between October 2004 and June 2005. Used as the test organism in these tests was the zebrafish Brachydanio rerio Hamilton - Buchanon (Cyprinidae. The acute toxicity test of 96/h duration showed that the tested samples had a slight acutely toxic effect on B. rerio, except for the sample of influent wastewater into the Cvetojevac wastewater treatment plant, which had moderately acute toxicity, indicating that such water should be prevented from entering the system in order to eliminate its detrimental effect on the purification process.

  17. Comparative analysis reveals that polyploidy does not decelerate diversification in fish.

    Science.gov (United States)

    Zhan, S H; Glick, L; Tsigenopoulos, C S; Otto, S P; Mayrose, I

    2014-02-01

    While the proliferation of the species-rich teleost fish has been ascribed to an ancient genome duplication event at the base of this group, the broader impact of polyploidy on fish evolution and diversification remains poorly understood. Here, we investigate the association between polyploidy and diversification in several fish lineages: the sturgeons (Acipenseridae: Acipenseriformes), the botiid loaches (Botiidae: Cypriniformes), Cyprininae fishes (Cyprinidae: Cypriniformes) and the salmonids (Salmonidae: Salmoniformes). Using likelihood-based evolutionary methodologies, we co-estimate speciation and extinction rates associated with polyploid vs. diploid fish lineages. Family-level analysis of Acipenseridae and Botiidae revealed no significant difference in diversification rates between polyploid and diploid relatives, while analysis of the subfamily Cyprininae revealed higher polyploid diversification. Additionally, order-level analysis of the polyploid Salmoniformes and its diploid sister clade, the Esociformes, did not support a significantly different net diversification rate between the two groups. Taken together, our results suggest that polyploidy is generally not associated with decreased diversification in fish - a pattern that stands in contrast to that previously observed in plants. While there are notable differences in the time frame examined in the two studies, our results suggest that polyploidy is associated with different diversification patterns in these two major branches of the eukaryote tree of life. © 2014 The Authors. Journal of Evolutionary Biology © 2014 European Society For Evolutionary Biology.

  18. Morphological and genetic evidence for multiple evolutionary distinct lineages in the endangered and commercially exploited red lined torpedo barbs endemic to the Western Ghats of India.

    Science.gov (United States)

    John, Lijo; Philip, Siby; Dahanukar, Neelesh; Anvar Ali, Palakkaparambil Hamsa; Tharian, Josin; Raghavan, Rajeev; Antunes, Agostinho

    2013-01-01

    Red lined torpedo barbs (RLTBS) (Cyprinidae: Puntius) endemic to the Western Ghats Hotspot of India, are popular and highly priced freshwater aquarium fishes. Two decades of indiscriminate exploitation for the pet trade, restricted range, fragmented populations and continuing decline in quality of habitats has resulted in their 'Endangered' listing. Here, we tested whether the isolated RLTB populations demonstrated considerable variation qualifying to be considered as distinct conservation targets. Multivariate morphometric analysis using 24 size-adjusted characters delineated all allopatric populations. Similarly, the species-tree highlighted a phylogeny with 12 distinct RLTB lineages corresponding to each of the different riverine populations. However, coalescence-based methods using mitochondrial DNA markers identified only eight evolutionarily distinct lineages. Divergence time analysis points to recent separation of the populations, owing to the geographical isolation, more than 5 million years ago, after the lineages were split into two ancestral stocks in the Paleocene, on north and south of a major geographical gap in the Western Ghats. Our results revealing the existence of eight evolutionarily distinct RLTB lineages calls for the re-determination of conservation targets for these cryptic and endangered taxa.

  19. Potential of zebrafish as a model for exploring the role of the amygdala in emotional memory and motivational behavior.

    Science.gov (United States)

    Perathoner, Simon; Cordero-Maldonado, Maria Lorena; Crawford, Alexander D

    2016-06-01

    Emotion is a key aspect of behavior, enabling humans and animals to assign either positive or negative values to sensory inputs and thereby to make appropriate decisions. Classical experiments in mammalian models, mainly in primates and rodents, have shown that the amygdala is essential for appetitive and aversive associative processing and that dysfunction of this brain region leads to various psychiatric conditions, including depression, generalized anxiety disorder, panic disorder, phobias, autism, and posttraumatic stress disorder. In the past 2 decades, the zebrafish (Danio rerio; Cyprinidae) has emerged as a versatile, reliable vertebrate model organism for the in vivo study of development, gene function, and numerous aspects of human pathologies. Small size, high fecundity, rapid external development, transparency, genetic tractability, and high genetic and physiologic homology with humans are among the factors that have contributed to the success with this small fish in different biomedical research areas. Recent findings indicate that, despite the anatomical differences in the brain structure of teleosts and tetrapods, fish possess a structure homologous to the mammalian amygdala, a hypothesis that is supported by the expression of molecular markers, analyses of neuronal projections in different brain areas, and behavioral studies. This Review summarizes this evidence and highlights a number of relevant bioassays in zebrafish to study emotional memory and motivational behavior. © 2016 Wiley Periodicals, Inc.

  20. Indigenous fish species in the modern ichthyofauna of the Balkhash basin

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    Nadir Shamilevich Mamilov

    2015-11-01

    Full Text Available Indigenous fish fauna of the Balkhash basin was mostly formed in the postglacial period and consists of 10 species from Cyprinidae family, 5 from Balitoridae, and 1 from Percidae. More than 20 alien fish species were introduced here during XXth century that led to eradication of indigenous fishes from the Balkhash Lake and the Ili River. Our investigations of the fish fauna during last 25 years revealed permanent shortage of living area of indigenous fishes. Nowadays fish communities from only indigenous fish species exist in some remote and isolated water bodies. Areas of all indigenous fish species are become disconnected. Reduction of habitats goes relatively slow for naked osman Gymnodiptychus dybowskii (Kessler, 1874, spotted thicklip loach Triplophysa strauchii (Kessler, 1874, and gray loach Triplophysa dorsalis (Kessler, 1872. Drastic reductions of areas were revealed for Ili marinka Schizothorax pseudoaksaiensis Herzenstein 1889, Balkhash marinka Schizothorax argentatus Kessler 1874, Severtsov’s loach Triplophysa sewerzowii (G.Nikolskii, 1938, Seven River’s minnow Phoxinus brachyurus Berg 1912, Balkhsh minnow Rhynchocypris poljakowii Kessler 1879, and Balkhash perch Perca schrenkii Kessler 1874. Marinkas, osmans and perch often become victims of overfishing and poaching of local people. In that region water resources usually are used by wasteful way and loaded with pollutants. Many indigenous fish species are able to bear relatively high level of environment pollution. Hence, the main threats for indigenous fishes are introductions of trout and sander, habitats lose and unstable hydrological regimen.

  1. Population Aspects of Fishes in Geba and Sor Rivers, White Nile System in Ethiopia, East Africa

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    Simagegnew Melaku

    2017-01-01

    Full Text Available This study was carried out to assess the diversity, condition factor, length-weight relationship, and sex ratio of fishes in Geba and Sor Rivers located in Baro-Akobo Basin, White Nile system within Ethiopia. Fish samples were collected in one wet and one dry season. The length-weight relationships were fitted using power equation for the most abundant species. A total of 348 fish specimens were collected using gillnets and hooks. These were identified into eight species and one Garra sp. representing seven genera and four families. Family Cyprinidae was the most dominant with six species (66.7%. Labeobarbus intermedius, Labeobarbus nedgia, and Labeo cylindricus were the most abundant fish species, respectively, with 60.72%, 16.83%, and 14.66% index of relative importance (IRI. The diversity index was higher for Geba River (H′ = 1.50 than for Sor River (H′ = 1.10. All the three most abundant species had negative allometric growth. Seasonal variations in the mean Fulton condition factor (FCF were statistically significant for L. cylindricus (p<0.05. There was variation in the sex ratio with the females dominating in all the three most abundant species. Further investigation into the fish diversity, food, feeding, and reproductive behaviors of fish species especially in the tributaries of these rivers and their socioeconomic aspects is recommended.

  2. RESEARCH ON ARGES RIVER FISH FAUNA IN BUDEASA-GOLESTI AREA

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    Alina-Mihaela Truţă

    2015-12-01

    Full Text Available Arges River was subject to periodic ichthyologic, hydrobiological and hydrological research. By its content and approach the present paper shows a series of research on fish fauna in Budeasa-Golesti area of Arges River, Pitesti. By research presented in the study we sought to evaluate the state and evolution of fish fauna in the city reservoirs, Pitesti area, over the last 30 years, trying to highlight the causes that led to the current situation and to propose measures for the conservation of natural fish fauna in the future. Fish fauna in Pitesti area currently consists of 14 species belonging to four families: Cyprinidae (9 species, Cobitidae (1 species, Esocidae (1 species and Percidae (3 species. Most species live naturally in lakes studied except for one species Pseudorasbora parva which was introduced accidentally. The research undertaken to reflect changes in the fish fauna in the last 30 years, indicates an increase in the number of species, either through deliberate stocking for sport fishing purposes or due to changes in biotope favouring the development of certain species which were accidental in the past.

  3. The influence of magnetic field on the spatial orientation in zebrafish Danio rerio (Hamilton and roach Rutilus rutilus (L.

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    Anastasia A. Batrakova

    2015-11-01

    Full Text Available Known, that some teleostei can perceive the geomagnetic field (GMF. However, the information about magnetosensitivity in Cyprinidae fish from artificial and natural habitats is obscure. We have registered preferred directions in Danio rerio (Hamilton from aquaria-cultivated line exposed to the natural GMF, 180 degrees reversal of horizontal GMF component, 180 degrees reversal of vertical GMF component, 180 degrees reversal of both vertical and horizontal GMF components and 90 degrees clockwise turn of horizontal GMF component. We also registered the preferred directions in Rutilus rutilus (L. from Rybinsk reservoir exposed to the natural GMF and 90 degrees clockwise turn of horizontal GMF component. It was found that zebrafish prefer two opposite directions towards east and west in the natural GMF. When the horizontal component of GMF was turned 90 degrees clockwise D. rerio prefer two opposite directions towards north and south. The possible reason of bimodality in zebrafish’s preferred directions distributions is discussed. The only direction towards east-north-east observed in roach under the natural GMF. This direction coincided with the way from the place of capture to the streamflow part of Rybinsk reservoir. And it was changed by south-south-east direction when turned the horizontal component of GMF 90 degrees clockwise. The possible reason of the choosing directions by fish with GMF is discussed.

  4. Significant genetic differentiation between native and introduced silver carp (Hypophthalmichthys molitrix) inferred from mtDNA analysis

    Science.gov (United States)

    Li, S.-F.; Xu, J.-W.; Yang, Q.-L.; Wang, C.H.; Chapman, D.C.; Lu, G.

    2011-01-01

    Silver carp Hypophthalmichthys molitrix (Cyprinidae) is native to China and has been introduced to over 80 countries. The extent of genetic diversity in introduced silver carp and the genetic divergence between introduced and native populations remain largely unknown. In this study, 241 silver carp sampled from three major native rivers and two non-native rivers (Mississippi River and Danube River) were analyzed using nucleotide sequences of mitochondrial COI gene and D-loop region. A total of 73 haplotypes were observed, with no haplotype found common to all the five populations and eight haplotypes shared by two to four populations. As compared with introduced populations, all native populations possess both higher haplotype diversity and higher nucleotide diversity, presumably a result of the founder effect. Significant genetic differentiation was revealed between native and introduced populations as well as among five sampled populations, suggesting strong selection pressures might have occurred in introduced populations. Collectively, this study not only provides baseline information for sustainable use of silver carp in their native country (i.e., China), but also offers first-hand genetic data for the control of silver carp in countries (e.g., the United States) where they are considered invasive.

  5. Miliç Irmağı (Terme, Samsun Balık Faunası.

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    Selma Uğurlu

    2015-12-01

    Full Text Available Miliç Irmağı’nda yaşayan balık türlerini ortaya çıkarmak amacıyla yapılan bu araştırma, Nisan 2004–Temmuz 2005 tarihleri arasında gerçekleştirilmiştir. Balık örneklerini toplamak amacıyla ırmak boyunca, ırmağın ekolojik karakterlerini temsil eden 7 istasyon belirlenmiştir. Çalışma süresince toplam 286 balık örneği; elektro şok aleti, balık kepçeleri, balık ağları, serpme ve oltalar aracılığıyla yakalanmıştır. Bu çalışmada 5 familyaya ait (Cyprinidae, Mugilidae, Syngnathidae, Blenniidae, Gobiidae 16 tür teşhis edilmiştir

  6. Fish composition and assemblage in the anthropogenic-modified tidally-restricted Doñana (Spain) marshlands

    Science.gov (United States)

    Moreno-Valcárcel, Raquel; Oliva-Paterna, Francisco J.; Arribas, Carmen; Fernández-Delgado, Carlos

    2013-03-01

    The Guadalquivir estuary is the largest estuarine area on the southern Atlantic coast of Europe; its anthropogenic tidally-restricted marshes are partly within the boundary of the Doñana National Park, southern Spain. Our two-year study describes the spatial and temporal patterns of the fish assemblages in the Doñana marshlands in terms of species richness, abundance and biomass. The main families were Mugilidae and Cyprinidae, which accounted for 40.9% of the total species richness. Unlike the fish assemblages found in other European estuaries, Doñana was dominated in both biomass and abundance by freshwater species, mainly invasive exotic species. The spatial analysis of the assemblage showed four significant fish groups corresponding to different habitats established a priori and related to the salinity gradient. Assemblages did not show a seasonal pattern and the temporal fish groups observed were mainly related to the hydrological cycle and the extreme drought that occurred during the study period.

  7. Freshwater fish Fauna and Restock Fish Activities of Reservoir in the Dardanelles (Canakkale-Turkey

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    Hüseyin SASI

    2012-06-01

    Full Text Available Turkey has, with geographic location including Istanbul and Çanakkale straits the system, 178,000 km in length streams, 906,000 ha of natural lakes, and 411,800 ha of dam lakes, and 28,000 ha of ponds due to richness inland waters which include freshwater fish. The fingerling fish (fry were restocked approximately 250,000,000 in natural lakes, dam lakes and ponds for fisheries between years of 1979 and 2005. Canakkale has rich freshwater potential with 7 major rivers (Büyükdere, Karamenderes stream, Kavak brook, Kocacay stream, Sarıcay stream, Tuzla brook, Umurbey brook, 7 Dam Lakes (Atikhisar, Zeytinlikoy, Bayramic, Bakacak, Tayfur, Umurbey and Yenice-Gönen Dam lakes. In the studies, it has been determined that 15 fish species belonging to 6 families (Anguillidae, Atherinidae, Salmonidae, Cobitidae, Cyprinidae and Poecilidae can be found in reservoirs. Fish restocking of the activities of the reservoir until today approximately 1,120,000 (Cyprinus carpio L., 1758 is introduced. In this study, the activity of Canakkale province in the fish restocking and reservoir exploiting possibilities were discussed in view of reservoir fisheries potential which is used insufficiently today.

  8. Seasonal variation and community structure of fishes in the Mahananda River with special reference to conservation issues

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    Shams Muhammad Galib

    2016-04-01

    Full Text Available This study was carried out in the Mahananda River from January to December 2013 with a view to determining the seasonal variation and community structure of fishes along with some conservation issues. Monthly sampling was carried out using traditional fishing gears and fishes were identified based on morphometric and meristic characters. A total of 4082 individuals of native fish species were captured, analyzed and classified into 62 species belonging to 46 genera, 25 families and 9 orders. Cypriniformes and Siluriformes were the dominant fish orders represented by 19 species each and the most abundant family was Cyprinidae (14 species. In addition to indigenous individuals, 9 individuals of 2 exotic fish species (Hypophthalmichthys molitrix and Pangasius hypophthalmus were also recorded. Among three sampling sites, S-1 was the most diversified in terms of not only the number of individual fish but also the number of species present represented by mean (±SE individuals of 151.50±25.22 and species of 25.58±3.91. Three distinct fish groups of fish families were revealed from the cluster analysis of similarity. To improve the situation, control of illegal fishing gears, establishment of sanctuaries and legal protection for threatened species are recommended.

  9. Fish assemblage structure and relations with environmental conditions in a Rocky Mountain watershed

    Science.gov (United States)

    Quist, M.C.; Hubert, W.A.; Isaak, D.J.

    2004-01-01

    Fish and habitat were sampled from 110 reaches in the Salt River basin (Idaho and Wyoming) during 1996 and 1997 to assess patterns in fish assemblage structure across a Rocky Mountain watershed. We identified four distinct fish assemblages using cluster analysis: (1) allopatric cutthroat trout (Oncorhynchus clarki (Richardson, 1836)); (2) cutthroat trout - brook trout (Salvelinus fontinalis (Mitchell, 1814)) - Paiute sculpin (Cottus beldingi Eigenmann and Eigenmann, 1891); (3) cutthroat trout - brown trout (Salmo trutta L., 1758) - mottled sculpin (Cottus bairdi Girard, 1850); and (4) Cyprinidae-Catostomidae. The distribution of fish assemblages was explained by thermal characteristics, stream geomorphology, and local habitat features. Reaches with allopatric cutthroat trout and the cutthroat trout - brook trout - Paiute sculpin assemblage were located in high-elevation, high-gradient streams. The other two fish assemblages were generally located in low-elevation streams. Associations between habitat gradients, locations of reaches in the watershed, and occurrence of species were further examined using canonical correspondence analysis. The results suggest that stream geomorphology, thermal conditions, and local habitat characteristics influence fish assemblage structure across a Rocky Mountain watershed, and they provide information on the ecology of individual species that can guide conservation activities. ?? 2004 NRC Canada.

  10. Feeding habits of an endemic fish, Oxygymnocypris stewartii, in the Yarlung Zangbo River in Tibet, China

    Science.gov (United States)

    Huo, Bin; Xie, Cong Xin; Madenjian, Charles P.; Ma, Bao Shan; Yang, Xue Feng; Huang, Hai Ping

    2014-01-01

    Feeding habits of Oxygymnocypris stewartii were investigated based on monthly sampling in the Yarlung Zangbo River from August 2008 to August 2009. The gut contents of 194 individuals were analysed and quantified with numerical and gravimetric methods. This species can be considered a generalized and opportunistic predator feeding both on teleosts and aquatic insects. A multivariate analysis revealed noticeable variation in O. stewartii diet composition with fish size and season. The smaller specimens fed primarily on Cobitidae and Hydropsychidae. As they grew, Cyprinidae and Chironomidae larvae became important prey. The preferred food items were teleosts in summer and autumn. For winter and spring, the predominant prey switched to chironomidae larvae. Diet composition did not significantly vary between the sexes. Finally, a significant and positive correlation between predator and prey length was found. These findings provide the fundamental information better understanding the role of this important endemic species in the Yarlung Zangbo River food web.

  11. Notes on the occurrence of Trypanosoma sp. (Kinetoplastida: Trypanosomatidae in freshwater fishes from South Africa

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    Maryke L. Ferreira

    2013-03-01

    Full Text Available A total of 257 fishes from four families, Clariidae, Cichlidae, Cyprinidae and Schilbeidae were collected from three localities: the Sand River Dam, Swaziland; the Nylsvlei Nature Reserve, South Africa and the Vaal Dam and Vaal River Barrage, South Africa. Only fishes (n= 154 from Clariidae and Cichlidae were found to be infected with trypanosomes. A total of 221 Clarias gariepinus (Burchell 1822 were collected from the Vaal Dam and Vaal Barrage area, South Africa. Of these, 74%(89/121 were infected with trypanosomes from the Vaal Dam and 63%(63/100 from the Vaal River Barrage, with no seasonal infection pattern. A prevalence of 25%(1/4 was found in C. gariepinus from the Sand River Dam, Swaziland, and a 50% (1/2 prevalence was found in Tilapia sparrmanii from the Nylsvlei Nature Reserve, South Africa. Standard measurements conformed closely to the morphometric and morphological descriptions of Trypanosoma mukasai. This article provides new locality records for T. mukasai from the Vaal Dam, Vaal River Barrage and Nylsvlei Nature Reserve (South Africa and the Sand River Dam (Swaziland. Tilapia sparrmanii collected in the Sand River Dam in Swaziland is also noted as a new host record.

  12. KEANEKARAGAMAN JENIS IKAN DI SUNGAI BATANG GADIS MANDAILING NATAL SUMATERA UTARA

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    Yusni Atifah

    2017-12-01

    Full Text Available Batang Gadis River, located in Mandailing Natal, faces a severe threat by the increase of human activities along the river. The fish community, as well as other biotas, is exposed to reducing environmental condition. Therefore, the study of fish community in this river is essential to get the most recent condition as a baseline data. The purpose of this research was to determine the species diversity of fish in Batang Gadis River of Mandailing Natal. This research used a descriptive exploratory method by surveying with purposive sampling method to collect samples at four specified stations. This study used a fishing net with a zig-zag pattern of sampling covered the river bank for about 200–300m at each observation station. The collected fish samples were identified at the Biology Laboratory of the University of Muhammadiyah Tapanuli Selatan. This study found ten species of fish from five families. The family with the most significant members was Cyprinidae (6 species, and the other families, Bagridae, Clariidae, Nemachelidae, and Loricariidae, were found with only one species. The species diversity comprised of Rasbora lateristriata, Nemacheilus fasciatus, Mystacoleucus marginatus, Tor tambra, Cyprinus carpio, Clarias bathracus, Rasbora argyrotaenia , Barbonymus gonionotus, Puntius binotatus, Bagrus nemurus, and Pterygoplichthys pardalis. The fish population of Batang Gadis River has diversity index (H’ of 1.77 and similarity index (E of 0.77.

  13. The influence of environmental abiotic factors on the qualitative and quantitative structure of ichthyofauna from predeltaic Danube area

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    Petronela Georgiana Calin Sandu

    2013-05-01

    Full Text Available The aim of the paper  is to analyse the influence of environmental factors on the capture and fish communities structure from Danube, between Siret River and Prut River mouth. Fish were collected from April to December 2012 in four fishing areas. During the year, 4910 kg, respectively 7121 fish of 31 species were collected. Cyprinidae, the dominant family, 54.84% in term of number of species, was represented by 17 species (Cyprinus carpio, Carassius gibelio, Barbus barbus, Abramis brama, Abramis sapa, Blicca bjoerkna, Leuciscus idus, Vimba vimba, Aspius aspius, Pelecus cultratus, Chondrostoma nasus, Ctenopharingodon idella, Hypophthalmichthys molitrix, Hypophthalmichthys nobilis, Scardinius erythrophthalmus, Rutilus rutilus, Alburnus alburnus. Other families had the following structure: Percidae (16.13% with 5 species (Sander lucioperca, Zingel zingel, Zingel streber, Perca fluviatilis, Gymnocephalus schraetzer, Acipenseridae (12.90% with 4 species (Huso huso, Acipenser stellatus, Acipenser ruthenus, Acipenser gueldenstaedtii, Clupeidae (6.45% with two species (Alosa immaculata, Alosa tanaica, Siluridae (3.23% with one species (Silurus glanis, Esocidae (3.23% with one species (Esox lucius  and Salmonidae (3.23% with one species also (Salmo labrax. The highest capture was 2977.93 kg (60.65%, during the spring season (April-May, followed by autumn season (September–November, with 992.39 kg (20.21%. The water level and water flow showed high correlation with both number and total catch, respectively.

  14. THE QUALITY OF WATER AND ICHTHIOFAUNA OF THE SOUTHERN LAKE IN OSIJEK – THE CONDITIONS FOR BIOMANIPULATION

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    Melita Mihaljević

    1996-09-01

    Full Text Available The Southern lake is shallow (deep up to 4 meters artificial lake with the ackerage of 6. 25. It is situated within the city of Osijek. Its reconstruction began in 1995, after it has been polluted for many years. The results of, hydrobiological examination show the basic ecological characteristics of the Southern lake: high transparency, great quantity of nutrients and highly developed macrophit vegetation. After the conducted analysis of the qualitative composition of the ichthiofauna, it has been found that the lake has 13 species of fish form six families, with the greatest number of them coming from Cyprinidae family. idae family. The fish species that outnumbers all other in the quantitative composition of the ichtiofauna is P. parua, but also well represented are L. gibosus, C. carrasius gibelio, Scardinius erythrophthalmus and Rutilus rutilus. In the trophic structure, the ratio of the predatory species is disadvantageous in relation to the unpredatory species. The application of the biomanipulation method has been suggested for the further reconstruction of the Southern lake: the quantity of the macrophit vegetation should be reduced by the way of its direct removal and the input of the herbivore fish species. The disadvantageous trophic ratio should be changed by overfishing of predatory species within the ichthiofauna and the input of unpredatory fish species.

  15. Phylogenetic Relationships of Pseudorasbora, Pseudopungtungia, and Pungtungia (Teleostei; Cypriniformes; Gobioninae Inferred from Multiple Nuclear Gene Sequences

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    Keun-Yong Kim

    2013-01-01

    Full Text Available Gobionine species belonging to the genera Pseudorasbora, Pseudopungtungia, and Pungtungia (Teleostei; Cypriniformes; Cyprinidae have been heavily studied because of problems on taxonomy, threats of extinction, invasion, and human health. Nucleotide sequences of three nuclear genes, that is, recombination activating protein gene 1 (rag1, recombination activating gene 2 (rag2, and early growth response 1 gene (egr1, from Pseudorasbora, Pseudopungtungia, and Pungtungia species residing in China, Japan, and Korea, were analyzed to elucidate their intergeneric and interspecific phylogenetic relationships. In the phylogenetic tree inferred from their multiple gene sequences, Pseudorasbora, Pseudopungtungia and Pungtungia species ramified into three phylogenetically distinct clades; the “tenuicorpa” clade composed of Pseudopungtungia tenuicorpa, the “parva” clade composed of all Pseudorasbora species/subspecies, and the “herzi” clade composed of Pseudopungtungia nigra, and Pungtungia herzi. The genus Pseudorasbora was recovered as monophyletic, while the genus Pseudopungtungia was recovered as polyphyletic. Our phylogenetic result implies the unstable taxonomic status of the genus Pseudopungtungia.

  16. Morphological and genetic evidence for multiple evolutionary distinct lineages in the endangered and commercially exploited red lined torpedo barbs endemic to the Western Ghats of India.

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    Lijo John

    Full Text Available Red lined torpedo barbs (RLTBS (Cyprinidae: Puntius endemic to the Western Ghats Hotspot of India, are popular and highly priced freshwater aquarium fishes. Two decades of indiscriminate exploitation for the pet trade, restricted range, fragmented populations and continuing decline in quality of habitats has resulted in their 'Endangered' listing. Here, we tested whether the isolated RLTB populations demonstrated considerable variation qualifying to be considered as distinct conservation targets. Multivariate morphometric analysis using 24 size-adjusted characters delineated all allopatric populations. Similarly, the species-tree highlighted a phylogeny with 12 distinct RLTB lineages corresponding to each of the different riverine populations. However, coalescence-based methods using mitochondrial DNA markers identified only eight evolutionarily distinct lineages. Divergence time analysis points to recent separation of the populations, owing to the geographical isolation, more than 5 million years ago, after the lineages were split into two ancestral stocks in the Paleocene, on north and south of a major geographical gap in the Western Ghats. Our results revealing the existence of eight evolutionarily distinct RLTB lineages calls for the re-determination of conservation targets for these cryptic and endangered taxa.

  17. Fish fauna of the Brahmaputra River, Bangladesh: richness, threats and conservation needs

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    Shams Muhammad Galib

    2015-12-01

    Full Text Available The Brahmaputra River is one of the largest rivers in the world as well as in Bangladesh. The present study was carried out for a period of one year from January to December 2013 with a view to assessing the availability of fishes in the river with species emphasis on species richness, existing threats and conservation issues. Daytime and night sampling were carried out in three sites located along the upstream to downstream course of the river on a monthly basis. Three fishing gears including cast net, seine net and drag net and one fishing trap were employed to collect fishes. A total of 67 finfish species including 63 indigenous and 4 exotic/alien species have been recorded belonging to 46 genera, 24 families and 8 orders. Cypriniformes and Cyprinidae were the most dominating order (21 species family (15 species of native fishes. A small portion (2% of native fishes was globally threatened. Over one third of total species (38% were considered threatened to extinct species in Bangladesh. Population trend of over two third of total fish species was Declining in the river. Major threats were alien/invasive species, banned fishing gears and loss of habitats.

  18. Duplication of the dystroglycan gene in most branches of teleost fish

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    Giardina Bruno

    2007-05-01

    Full Text Available Abstract Background The dystroglycan (DG complex is a major non-integrin cell adhesion system whose multiple biological roles involve, among others, skeletal muscle stability, embryonic development and synapse maturation. DG is composed of two subunits: α-DG, extracellular and highly glycosylated, and the transmembrane β-DG, linking the cytoskeleton to the surrounding basement membrane in a wide variety of tissues. A single copy of the DG gene (DAG1 has been identified so far in humans and other mammals, encoding for a precursor protein which is post-translationally cleaved to liberate the two DG subunits. Similarly, D. rerio (zebrafish seems to have a single copy of DAG1, whose removal was shown to cause a severe dystrophic phenotype in adult animals, although it is known that during evolution, due to a whole genome duplication (WGD event, many teleost fish acquired multiple copies of several genes (paralogues. Results Data mining of pufferfish (T. nigroviridis and T. rubripes and other teleost fish (O. latipes and G. aculeatus available nucleotide sequences revealed the presence of two functional paralogous DG sequences. RT-PCR analysis proved that both the DG sequences are transcribed in T. nigroviridis. One of the two DG sequences harbours an additional mini-intronic sequence, 137 bp long, interrupting the uncomplicated exon-intron-exon pattern displayed by DAG1 in mammals and D. rerio. A similar scenario emerged also in D. labrax (sea bass, from whose genome we have cloned and sequenced a new DG sequence that also harbours a shorter additional intronic sequence of 116 bp. Western blot analysis confirmed the presence of DG protein products in all the species analysed including two teleost Antarctic species (T. bernacchii and C. hamatus. Conclusion Our evolutionary analysis has shown that the whole-genome duplication event in the Class Actinopterygii (ray-finned fish involved also DAG1. We unravelled new important molecular genetic details

  19. Brain structure evolution in a basal vertebrate clade: evidence from phylogenetic comparative analysis of cichlid fishes

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    Kolm Niclas

    2009-09-01

    Full Text Available Abstract Background The vertebrate brain is composed of several interconnected, functionally distinct structures and much debate has surrounded the basic question of how these structures evolve. On the one hand, according to the 'mosaic evolution hypothesis', because of the elevated metabolic cost of brain tissue, selection is expected to target specific structures mediating the cognitive abilities which are being favored. On the other hand, the 'concerted evolution hypothesis' argues that developmental constraints limit such mosaic evolution and instead the size of the entire brain varies in response to selection on any of its constituent parts. To date, analyses of these hypotheses of brain evolution have been limited to mammals and birds; excluding Actinopterygii, the basal and most diverse class of vertebrates. Using a combination of recently developed phylogenetic multivariate allometry analyses and comparative methods that can identify distinct rates of evolution, even in highly correlated traits, we studied brain structure evolution in a highly variable clade of ray-finned fishes; the Tanganyikan cichlids. Results Total brain size explained 86% of the variance in brain structure volume in cichlids, a lower proportion than what has previously been reported for mammals. Brain structures showed variation in pair-wise allometry suggesting some degree of independence in evolutionary changes in size. This result is supported by variation among structures on the strength of their loadings on the principal size axis of the allometric analysis. The rate of evolution analyses generally supported the results of the multivariate allometry analyses, showing variation among several structures in their evolutionary patterns. The olfactory bulbs and hypothalamus were found to evolve faster than other structures while the dorsal medulla presented the slowest evolutionary rate. Conclusion Our results favor a mosaic model of brain evolution, as certain

  20. Novel SINE families from salmons validate Parahucho (Salmonidae) as a distinct genus and give evidence that SINEs can incorporate LINE-related 3'-tails of other SINEs.

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    Matveev, Vitaliy; Nishihara, Hidenori; Okada, Norihiro

    2007-08-01

    Short interspersed elements (SINEs) constitute a group of retroposons propagating in the genome via a mechanism of reverse transcription, in which they depend on the enzymatic machinery of long retroposons (LINEs). Over 70 SINE families have been described to date from the genomes of various eukaryotes. Here, we characterize two novel SINEs from salmons (Actinopterygii: Salmonoidei). The first family, termed SlmI, was shown to be widespread among all genera of the suborder. These SINEs have a tRNA(Leu)-related promoter region at their 5'-end, a unique central conserved domain with a subfamily-specific region, and an end with RSg-1-LINE-derived 3'-terminus preceding the A/T-rich tail. The same LINE-related segment is also shared by two other salmonid SINEs: HpaI and OS-SINE1. The structural peculiarities and overall sequence identity of the SlmI 3'-terminus suggest that it has been acquired from HpaI SINEs but not directly from the partner LINE. This region plays a crucial role in the process of retrotransposition of short interspersed elements, and the case of its SINE-to-SINE transmission is the first recorded to date. Possible scenarios and potential evolutionary implications of the observed interaction between short retroposons are discussed. Apart from the above, we found a copy of the SlmI SINE in the GenBank entry for the blood fluke, Schistosoma japonicum (Trematoda: Strigeiformes) -- a trematode causing one of the most important human helminth infections, with its genome known to host other groups of salmonoid retroposons. In the present article, we suggest our views with regard to possible ways in which such an intensive horizontal transfer of salmonoid retroposons to the schistosomal genome occurs. The second novel SINE family, termed SlmII, originates from one of the SlmI subfamilies, with which it shares the same tRNA-related region, central domain, and a part of RSg-1-derived segment, but has a different 3'-tail of unidentified origin. Its distribution