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Sample records for cyclolepis waleckii rolik

  1. Genetic analysis of population differentiation and adaptation in Leuciscus waleckii.

    Science.gov (United States)

    Chang, Yumei; Tang, Ran; Sun, Xiaowen; Liang, Liqun; Chen, Jinping; Huang, Jinfeng; Dou, Xinjie; Tao, Ran

    2013-12-01

    Demographic events and natural selection both influence animal phenotypic and genetic variation; exploring the effects of demography and selection on population divergence is of great significance in evolutionary biology. To uncover the causes behind the patterns of genetic differentiation and adaptation among six populations of Leuciscus waleckii from Dali Basin (two populations, alkaline vs. freshwater) and Amur Basin (four populations, freshwater rivers vs. alkaline lake), a set of 21 unlinked polymorphic microsatellite markers and two mitochondrial DNA sequences (Cytb and D-loop) were applied to examine whether populations from different environments or habitats have distinct genetic differentiation and whether alkalinity is the major factor that caused population divergence. Bayesian analysis and principal component analysis as well as haplotype network analysis showed that these populations are primarily divided into two groups, which are congruent with geographic separation but not inconsistent with the habitat environment (alkalinity). Using three different approaches, outlier detection indicated that one locus, HLJYL017, may be under directional selection and involved in local adaptation processes. Overall, this study suggested that demographic events and selection of local environmental conditions including of alkalinity are jointly responsible for population divergence. These findings constitute an important step towards the understanding of the genetic basis of differentiation and adaptation, as well as towards the conservation of L. waleckii.

  2. Community Species Structure Characteristics of Four Types of Keteleeria cyclolepis Natural Forests in Fujian Province%福建江南油杉4种天然林群落物种结构特征

    Institute of Scientific and Technical Information of China (English)

    何国生

    2011-01-01

    Community species structure characteristics of four types of Keteleeria cyclolepis natural forests in Fu-jian Province, I. E. , K. Cyclolepis forest, K. Cyclolepis + Castanopsis eyrei forest, Keteleeria cyclolepis + Cyclobanop-sis gilva forest, and Keteleeria cyclolepis + Phyllostachys pubesces forest, were investigated and analyzed by means of typical square sampling method. The results showed that there were two sublayers in the arbor tree layer in all of the four types of Keteleeria cyclolepis natural forests, while K. Cyclolepis mainly occupied the upper part of the arbor tree layer, growing with 16 companion tree species. There were 10 tree species from the community building families of the broadleaved subtropical evergreen forests including Fagaceae, Lauraceae, Magnoliaceae and Theaceae among the companion tree species, accounting for 62. 5% of the total. There were 26 species in the shrub layer of different types of the natural forests, among which 9 shrub species were from the families of Lauraceae, Magnoliaceae and Theaceae, accounting for 34. 6% of the total. There were 7 species respectively in the herb layer and the inter-layer of the vegetation. There were not much differences in the species composition in the shrub and herb layers in different types of the natural forests, but the dominant species in the shrub and herb layers in different types of the natural forests were quite different. The dominant species in shrub layer and herb layer of Keteleeria cyclolepis forest were Itea chinensis and Woodwardia japonica respectively. Asidosasa notata and Rhododendron henryi were the co-dominant species in shrub layer, whereas Dicranopteris dichotoma was the dominant species in the herb layer of Keteleeria cyclolepis + Cyclobanopsis gilva forest type. Rhododendron ovatum and Eurya alata were the co-dominant species in the shrub layer of Keteleeria cyclolepis + Castanopsis eyrei forest and Keteleeria cyclolepis + Phyllostachys pubesces forest types

  3. Transcriptional Profiling Reveals Differential Gene Expression of Amur Ide (Leuciscus waleckii during Spawning Migration

    Directory of Open Access Journals (Sweden)

    Jun Cui

    2015-06-01

    Full Text Available Amur ide (Leuciscus waleckii, an important aquaculture species, inhabits neutral freshwater but can tolerate high salinity or alkalinity. As an extreme example, the population in Dali Nor lake inhabits alkalized soda water permanently, and migrates from alkaline water to neutral freshwater to spawn. In this study, we performed comparative transcriptome profiling study on the livers of Amur ide to interrogate the expression differences between the population that permanently inhabit freshwater in Ganggeng Nor lake (FW and the spawning population that recently migrated from alkaline water into freshwater (SM. A total of 637,234,880 reads were generated, resulting in 53,440 assembled contigs that were used as reference sequences. Comparisons of these transcriptome files revealed 444 unigenes with significant differential expression (p-value ≤ 0.01, fold-change ≥ 2, including 246 genes that were up-regulated in SM and 198 genes that were up-regulated in FW. The gene ontology (GO enrichment analysis and KEGG pathway analysis indicated that the mTOR signaling pathway, Janus kinase-signal transducer and activator of transcription (JAK-STAT signaling pathway, and oxidative phosphorylation were highly likely to affect physiological changes during spawning migration. Overall, this study demonstrates that transcriptome changes played a role in Amur ide spawning migration. These results provide a foundation for further analyses on the physiological and molecular mechanisms underlying Amur ide spawning migration.

  4. Transcriptional Profiling Reveals Differential Gene Expression of Amur Ide (Leuciscus waleckii) during Spawning Migration

    Science.gov (United States)

    Cui, Jun; Xu, Jian; Zhang, Songhao; Wang, Kai; Jiang, Yanliang; Mahboob, Shahid; Al-Ghanim, Khalid A.; Xu, Peng

    2015-01-01

    Amur ide (Leuciscus waleckii), an important aquaculture species, inhabits neutral freshwater but can tolerate high salinity or alkalinity. As an extreme example, the population in Dali Nor lake inhabits alkalized soda water permanently, and migrates from alkaline water to neutral freshwater to spawn. In this study, we performed comparative transcriptome profiling study on the livers of Amur ide to interrogate the expression differences between the population that permanently inhabit freshwater in Ganggeng Nor lake (FW) and the spawning population that recently migrated from alkaline water into freshwater (SM). A total of 637,234,880 reads were generated, resulting in 53,440 assembled contigs that were used as reference sequences. Comparisons of these transcriptome files revealed 444 unigenes with significant differential expression (p-value ≤ 0.01, fold-change ≥ 2), including 246 genes that were up-regulated in SM and 198 genes that were up-regulated in FW. The gene ontology (GO) enrichment analysis and KEGG pathway analysis indicated that the mTOR signaling pathway, Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway, and oxidative phosphorylation were highly likely to affect physiological changes during spawning migration. Overall, this study demonstrates that transcriptome changes played a role in Amur ide spawning migration. These results provide a foundation for further analyses on the physiological and molecular mechanisms underlying Amur ide spawning migration. PMID:26096003

  5. 东北雅罗鱼微卫星分子标记的筛选及特征分析%Isolation and Characterization of Microsatellite from Genome of Leuciscus waleckii Dybowski

    Institute of Scientific and Technical Information of China (English)

    刘金亮; 常玉梅; 徐丽华; 刘春雷; 梁利群; 韩国苍; 高玉奎; 柳玉海

    2011-01-01

    Magnetic beads enriched method was used to isolate microsatellite DNA from Leuciscus waleckii Dybowski genome. L. waleckii genomic DNA was extracted and then digested with restriction enzyme Sau 3A I. Targeted segments of 400 -900 base pairs were collected by centrifugation of sucrose density gradient and ligated to adaptors.Then the purified ligated DNA was hybridized with Biotin-labeled simple sequence repeats probes (CAG)8, (TGA)8,(AGAT)6 and (GATT)6. After capture of target fragments by magnetic beads and PCR amplification,the selected DNA s were cloned into the pMD18-T vector and transformed into competent Escherichia coli DH 5α,and finally a microsatellite library was obtained. One thousand, four hundred and sixteen positive colonies were obtain through twice screens by colony PCR. Sequencing of these positive colonies confirmed that 1 047 contained microsatellite loci( number of repeats ≥5). Among these sequences ,737 repeat motifs ( about 67.99% ) were perfect, 166 repeat motifs ( about 15.31% )were imperfect,and 181 repeat motifs ( about 16.70% )were compound. We design 105 pairs of primers from 160 microsatellite sequence and compose them. As a result,56 pairs were screened and used successfully to amplify special fragment, among which 23 pairs were polymorphism. Using 18 pairs of these microsatellite markers, we analyzed genetic structures of one L. waleckii population sampled from Dali Lake. The result showed that the alleles were ranged from 3 -11 ,while the polymorphic information content (PIC)were 0. 207 7 -0. 882 0. The statistic results indicated that 67% loci (12/18)were in high polymorphic level.%通过磁珠富集法筛选东北雅罗鱼的微卫星分子标记.采用限制性内切酶Sau 3AI对东北雅罗鱼基因组DNA进行酶切,选取400~900 bp的片段进行PCR全基因组扩增,并利用生物素标记的(CAG)8、(TGA)8、(AGAT)6、(GATT)6 4个探针进行微卫星片段的富集.将得到的片

  6. Dicty_cDB: Contig-U13097-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Value N ( BJ384847 ) Dictyostelium discoideum cDNA clone:ddc50n21, 3' ... 159 2e-35 1 ( AF287377 ) Barbus c...yclolepis strumicae isolate Bstrum1556G A... 44 0.95 1 ( AF287376 ) Barbus cyclol...epis strumicae isolate Bstrum1555G A... 44 0.95 1 ( AF287375 ) Barbus cyclolepis strumicae isolate Bcystru35

  7. Diversity of lampreys and fishes of the Upper Vistula River drainage, Poland: present state and future challenges

    Directory of Open Access Journals (Sweden)

    Michał Nowak

    2010-12-01

    Full Text Available There have been some 57 lamprey and fish species recorded in the Upper Vistula Riverdrainage (S-E Poland. Among these there are a number of species-complexes (Carassius auratus, Gobiogobio, Cobitis taenia with unresolved taxonomy. Identity of some others (Barbus waleckii, Romanogobioalbipinnatus, Cottus gobio is questionable and needs a review. Phylogenetic relationships of anotherones (Eudontomyzon mariae, Lampetra planeri, Lampetra fluviatilis is also under debate. Knowledgeabout the distribution of many species is very scarce and needs to be filled. In the current work webriefly summarise present state of the diversity and classification of lampreys and fishes of the UpperVistula drainage and point some urgent questions that have arose in recent years and are waiting fornew solutions.

  8. Comunidades Halófilas de la costa de la Bahía Blanca (Argentina: Caracterización, mapeo y cambios durante los últimos cincuenta años Halophytic communities of the coast of Bahía Blanca (Argentina: characterization, mapping and changes in the last fifty years

    Directory of Open Access Journals (Sweden)

    Ana Julia Nebbia

    Full Text Available Realizamos una clasificación y mapeo de las unidades de vegetación presentes en la franja costera de Bahía Blanca (Argentina, donde identificamos ocho tipos de ambientes naturales: cangrejales, charcos temporarios, zonas intermareales ocupadas por marismas de Sarcocornia perennis o espartillares de Spartina alterniflora , arbustales bajos de Allenrolfea patagonica , arbustales bajos de Atriplex undulata, arbustales altos de Cyclolepis genistoides y pastizales. Comparamos los valores obtenidos a partir de nuestros censos florísticos con datos provenientes de estudios fitosociológicos desarrollados en el área entre 1949 y 1950. Observamos que la composición y estructura de las comunidades se mantuvo relativamente constante, excepto en el caso del porcentaje de especies exóticas, donde se observa que los valores actuales son superiores en el caso de los tres ambientes de arbustal, lo que podría deberse a efectos de actividades humanas. Como conclusión se propone que, si bien este ambiente se encuentra en relativo buen estado de conservación, comparado con otros de la región, muestra signos de deterioro que justifican la adopción de medidas de conservación.We classified and mapped vegetation units along the coast of Bahía Blanca (Argentina, where we discriminated eight habitat types: intertidal flats without vegetation and with high density of crab burrows ( cangrejales , temporary ponds and puddles, intertidal steppes occupied by stands of Sarcocornia perennis or by Spartina alterniflora marshes, low Allenfolfea patagonica shrubs, low Atriplex undulata shrubs, high Cyclolepis genistoides shrubs and halophytic grasslands with Distichlis spicata . We compared our results with those from a phytosociological survey conducted in the area in 1949 and 1950. We observed a relative constancy in the composition and structure of plant communities, except for the percentage of exotic species being greater than historical ones for the three

  9. [Phylogenetic analysis of Altai osmans of the genus Oreoleuciscus (Pisces, Cyprinidae, Leuciscinae), based on the analysis of the cytochrome oxidase 1 gene (Co-1) sequence].

    Science.gov (United States)

    Batishcheva, N M; Kartavtsev, Iu F; Bogutskaia, N G

    2011-10-01

    Molecular genetic analysis of Altai osmans of the genus Oreoleuciscus from two different parts of the range was carried out. In this study, based on the mitochondrial Co-1 gene sequence, a total of 25 fish specimens belonging to four genera were examined: (1) O. humilis, 2 specimens; O. potanini, 13 specimens; (2) Pseudaspius leptocephalus, 1 specimen; (3) Tribolodon brandtii, T. hakonensis, and T. sachalinensis from the GenBank database, 8 speciens; and (4) Leuciscus waleckii, 1 specimen (used as an outgroup). The p-distances were very low both within and between the species: (1) 0.20 +/- 0.03%; (2) 0.40 +/- 0.12%; and (1-2) 0.80 +/- 0.04%. To visualize the relationships among all of the species examined, the neighbor joining (NJ), maximum parsimony (MP), Bayesian (BA), and maximum likelihood (ML) trees were constructed. The results obtained using these methods were very similar. It was demonstrated that species assignment of the individuals (barcoding) with the help Co-1 gene was effective, despite of very low divergence of the two osman taxa, which was comparable with typical intraspecific values in other animal groups. Taxonomic status of O. potanini and O. humilis requires further investigation with paying attention to low genetic distances between these species along with the lack of material from sympatric parts of the ranges.